LOCUS       DAOMZY010000001        35495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_891_length_35495_cov_6.241648, whole genome shotgun sequence.
ACCESSION   DAOMZY010000001 DAOMZY010000000
VERSION     DAOMZY010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 35495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35495
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_891_length_35495_cov_6.241648"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="PLK64_00005"
     CDS             complement(<1..1147)
                     /locus_tag="PLK64_00005"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HPV78150.1"
                     /translation="MTKEQQRAELHRTIWRIANDLRGSVDGWDFKSYVLGLLFYRFIS
                     EHLTAYINGLERAASVGDFDYADFSDEQAEFGREVTVEEKGYYILPSELFANVRARAP
                     HDPNLNETLERVFANIEGSAIGTDSEGDLKGLFDDLDVNSAKLGPTVARRNEKLVKLL
                     NAIGDLALGTFADNTIDAFGDAYEYLMTMYAGSAGKSGGEFFTPQEVSELLARITVVG
                     KTSVNKVYDPACGSGSLLLKFAKVLGKENVRQGFFGQEINLTTYNLARINMFLHDVNF
                     EQFDIAHGDTLIDPAHWDDEPFEAIVSNPPYSTRWAGADDQLLINDPRFAPAGVLAPK
                     SKADLAFTMHMLHWLAVNGTAAIVEFPGVLYRGGAEQKIRKYLIDNNY"
     gene            complement(1444..1517)
                     /locus_tag="PLK64_00010"
     tRNA            complement(1444..1517)
                     /locus_tag="PLK64_00010"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1481..1483),aa:Pro,seq:cgg)
     gene            complement(1779..2714)
                     /locus_tag="PLK64_00015"
     CDS             complement(1779..2714)
                     /locus_tag="PLK64_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPV78151.1"
                     /translation="MHPLARAAVGATAVGVLGVGYASLVERNLFVLRRHTLPVLPPGS
                     ASLRVLHLTDLHLMPAQARKIEWVRALAGLEPDLVVNTGDNIANPGSIEPLLRAFEPL
                     LGVPGVFVLGSNDYFAPRMKNPALYLTGNHRRGLGEASRLPTERLVAGLAAGGWDDLT
                     NRRTRLAVSGVPLEFVGVDDPHLDYDRYDVVAGAAADDVALTVGVTHAPYQRVLDAMT
                     ADGAGLVIAGHTHGGQLCLPGWGALVTNCDLDTRRAKGVSRWWPGAGGAPSAQAPQGA
                     AYLEVSAGLGTSPMAPVRFACRPEASLLTLVPRDS"
     gene            2794..3252
                     /locus_tag="PLK64_00020"
     CDS             2794..3252
                     /locus_tag="PLK64_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HPV78152.1"
                     /translation="MSTLKETLHADLTEAIRSRNAVVAATLRMALTAISTAETAGTTH
                     RELSDDEVLKVLVKEGKKRREASAAYTGAGRPELAAREDAEFAVLEGYLPTQLTDEEI
                     AALVAQAVAQTGATGMPQLGMVMKAVQPLVAGRADGGRVAAAVRVALTAG"
     gene            complement(3249..5642)
                     /locus_tag="PLK64_00025"
     CDS             complement(3249..5642)
                     /locus_tag="PLK64_00025"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPV78153.1"
                     /translation="MSGRTTNVGGVVSLLGAFLAAAVITGFLSAGLFLPAVGAIGGSV
                     RTVVDLFDSLPDNFTTSPLAEQSVILAADGTKIATPYDENRVIVPLNEIASVMRTAQV
                     AIEDHRFYEHGGADLQGILRAFISNQATGELTGGGSTLTQQYVKITLQENALRAGDKA
                     GAAAATARNYGRKIQELKYAIQLEKTLTKDQILEGYLNLVYYGDRAYGVEAASQHYFG
                     HGAKTLNLQEAALLAGIVQNPGTSDPVNFPEKSLARRNVVLDRMKDLGLIKDAEWKAA
                     RAVPLAAMLKVTPAKNSCQSSDYPYFCDYVLAWLKQDPSLDAVLGRGEAQRVQAIYRG
                     GLTIQTTLDPAMARVANEEITRKVPIGNDEEIGSAAAILDTKTGAVKAIGQNTTYSVK
                     PANRGETAVNWSVDSRYGGSIGFSFGSTAKIFTLVTALEAGWPTTSSVFAKAASPSKG
                     ADYTSKEIHGACGLGGGGWNVRNDINFPEGDISLAEATAKSINTAFVGMAIQLGGDPC
                     KIRDTGWRMGLHQGNGKEIPPYPSAIILGTWSASPMTMASTYQTIANEGVYCPPIPVM
                     SIVKDGKALPLPALGSACEKRVDPEVARGVTSLLEGVLRNGGTADNAGLVGGRPAAGK
                     TGTTDNHNETWFVGYTPQLTTAVWVGTPDDKNNARRVTDLCMRQMTETKGCSAGRYAA
                     LFGMTIAAPVWKSIMDRALEGQPKVPFISPGTQIVNGEKVDIPDIARRSLDDAKALLL
                     QSGFVPSVVKVYSNYRVGTVVGTTPKNQATRGSVVKILVSQGPAPAPTAPPEPAPPG"
     gene            5925..6233
                     /locus_tag="PLK64_00030"
     CDS             5925..6233
                     /locus_tag="PLK64_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HPV78154.1"
                     /translation="MSTPILRGALRQDWAGLGACVSGDPDDLFVKGAAQQQAKEVCQR
                     CPVIAECLAEALDNRTEFGVWGGMTERERRALLRRNPTVTSWWAMFAHAGAGRADVAS
                     "
     gene            complement(6286..7437)
                     /locus_tag="PLK64_00035"
     CDS             complement(6286..7437)
                     /locus_tag="PLK64_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HPV78155.1"
                     /translation="MARTPVLDIDAMLADRHTRIVVCCGSGGVGKTTTAAALALRAAE
                     AGREVVVLTIDPARRLAQSLGLTELDNVPRPVSGLADDAVPGGRLDAMMLDMKRTFDE
                     VVLAHSTREAAEQILANPFYQAVSSSFAGTQEYMAMEKLGQLRARADREGTWDLIVVD
                     TPPSRSALDFLDAPKRLGSFLDGRFIRLIAAPAKMGGRAGLRLVNVGIGLVSKTLTTV
                     LGGEMLRDVQTFVNALDTMFGGFRERADLTYALLKEGGTEFIVVAAPERDALREASYF
                     VARLEQEGMPLAGVVVNRMQPSAAPGLTAARARAAAEQLAEADRPAAAAALSLHAEVS
                     ELAARHRNLVRRFTAGHPSIPVVEVASLAQDVHDADGLREVGRRLAGLP"
     gene            complement(7437..8489)
                     /locus_tag="PLK64_00040"
     CDS             complement(7437..8489)
                     /locus_tag="PLK64_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HPV78156.1"
                     /translation="MPPAPDPLRADGFAGVRLHVVTGKGGTGKTTVAAALAVALANEG
                     LRVLLAEVEGRQGISQTFDVPPLPTTEVRVAGGAQGGEVVGLAVDAKTALLEYLQLFY
                     KLGRAGGVLERFGAIDFATTIAPGVRDLLLIGRVYEASRRRHDAKHHRGSPPEELAYD
                     AIVLDAPPTGRIPRFLSVGAEVAGLARMGPIHSQAGSITELLHSDATRVHVVTLLEEM
                     PVQETVDAVAELRELGMPVGAVVINQAQAREDLDALGAFGGLDAPLNSDPTAAAKLSA
                     DLEAVGLDLPRATVEGLLDEVHRHTERVALEVEQLTVLEQLGQPLVTVPFLIDGIDLD
                     AVTEIAALLAEQGMVP"
     gene            8580..8747
                     /locus_tag="PLK64_00045"
     CDS             8580..8747
                     /locus_tag="PLK64_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HPV78157.1"
                     /translation="MTSWEYATVPLIVHATKAILDNWGRDGWELVQVVPGPDGNGLVA
                     YLKRPLAGGQA"
     gene            8744..9217
                     /locus_tag="PLK64_00050"
     CDS             8744..9217
                     /locus_tag="PLK64_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPV78158.1"
                     /translation="MSAVEERLAAMGYSVPDVVPPVAAYVPALRQGDWVFVSGQLPMR
                     DGAMPATGKVGDGPGQVSPEAAKQLAAFCALNGIAAVKSVIGDLDKVVRIVKVVGFVA
                     ADPSFTGVPGVVNGASELLGAVFGDAGVHARSAVGVSVLPLDAPVEVELIVAVRD"
     gene            9232..10110
                     /locus_tag="PLK64_00055"
     CDS             9232..10110
                     /locus_tag="PLK64_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPV78159.1"
                     /translation="MIRDFSTSNAPALHATALAWLSGEHGEPAPLRPAATVMIVRDAS
                     DDESGNSGGSPVEVFLLRRVASMAFAPSTIVFPGGGVDPRDSAELPWAGPGVLEWSVR
                     LGADEEATRKLVVAAVREVFEECGVLFASADEDGPLVEVSSPQWQQVRAGLVARELSL
                     SDVLREHGLCLRTDLLRAHAHWVTPVCEPRRFDTRFFVALMPAGRAIDGATSEADHAG
                     WFDPASVLADYAAGSAVVLPPTLVCLEDLAASPSAAEFFAQEPVIRRIMPELVVNDAG
                     EAVMRTDFAGTKAPRG"
     gene            10200..10937
                     /locus_tag="PLK64_00060"
     CDS             10200..10937
                     /locus_tag="PLK64_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPV78160.1"
                     /translation="MTLDGTNTWVLREPGAQECIVVDPGPLDEAHLTRVLDVVRRDGG
                     RVGLTLLTHDHSDHADAAERFAHLSGAPVRAIRAIGTGHDDLADGDSVSVGGLDLVVV
                     TTPGHTSDSVSFLLPAECAVLTGDTILGRGTTVVAWPDGELAAYLASLERLEALCGSG
                     AVARILPGHGPHLPDAGAVLRYYLEHRAQRLEQVRGAVAAGARTPWEVVETVYADVPR
                     AVWGAAEWSVRAQLAYLAERGEEGQLG"
     gene            complement(10934..11812)
                     /locus_tag="PLK64_00065"
     CDS             complement(10934..11812)
                     /locus_tag="PLK64_00065"
                     /inference="COORDINATES: protein motif:HMM:NF025446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4261 domain-containing protein"
                     /protein_id="HPV78161.1"
                     /translation="MGLIDKIRSSLPIPGRSFRHPEPAAQLLAFVLLERTIGVDEIVA
                     LVATVPGASATPMAHSPSSAEATDPTETSGPVEITLGGTTGFVLEISAPVPDGEAEAS
                     AANNAFWPDAAAAVARHRAHLIVSVSDQRADGVGDADGADPSERHTQRYAAFAHLVAA
                     LLGHEATVGVYLGDQGVVYEPAFYIDAVAGAGDGLPWEVAWFTWAAWESEGVSCGSTR
                     GLRVFGHEELEVRGSTAAPSAVMDLLANIASYVVESGAVLMPGETLGYTDDQKLTITA
                     VEASFVEGHTLAIGLP"
     gene            complement(11867..12544)
                     /locus_tag="PLK64_00070"
     CDS             complement(11867..12544)
                     /locus_tag="PLK64_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPV78162.1"
                     /translation="MDHDVVRRAPLFAALDDEAGQALLAQMNSSRLERGDILFREGDP
                     GDTLYVIGEGKVKLGRTSADGRENLIAILGPGEMFGELSLFDPGPRTMTATAVAETQL
                     MGLGNDSLTELLTGRPEVAKALMAALAARLRRTNEHLADLVFTDVPGRVAKALLDLAN
                     RFGRPVEDGIMVAHDLTQEELAQLVGASRETVNKALADFATRGWLKLEARAVLLLDIE
                     RLKRRAR"
     gene            12674..13402
                     /gene="nth"
                     /locus_tag="PLK64_00075"
     CDS             12674..13402
                     /gene="nth"
                     /locus_tag="PLK64_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPV78163.1"
                     /translation="MPADRAAPASETPVALTRRARRIYRALLERYPYAHCELDFTTPL
                     ELLVATILSAQTTDVRVNQVTPILFARYRTAAEYAAADRADLEALLGPLGFFRAKTES
                     LLKLGAALVERFDGEVPGRLDDLVTLPGVGRKTANVVLGNAFGVPGITVDTHFGRLVR
                     RFGWTTEEDPVKVEHAVGALFPRKDWTMLSHVVIFHGRRTCHARKPACGACPVAQWCP
                     AYGTGETDPEKAARLLTYELAPTS"
     gene            13399..14121
                     /locus_tag="PLK64_00080"
     CDS             13399..14121
                     /locus_tag="PLK64_00080"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HPV78164.1"
                     /translation="MSSDVSRVAHGTPLVPLVPLGAGMPRPRPAWVDGILEGARAEPE
                     WFTRYLPPDGDQRQSAVLILFGPGPQGEDSLLFIERAHTLRSHAAQISFPGGGVDPED
                     DNVVAAALRESFEEVGLDPTGVDIVGTLPELFLPVSAYTVHPVLAWWKDPSPVYVGHP
                     DEVAQVLSVPVSYLADPANRHTVSHPSGYRGPAWDLGDDLLLWGFTGGIVNKILELAG
                     FAREWDSDHLVPLPERFMRRSS"
     gene            14118..15326
                     /locus_tag="PLK64_00085"
     CDS             14118..15326
                     /locus_tag="PLK64_00085"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HPV78165.1"
                     /translation="MTGGTLLDIVLIVLLGSYAVSGFGQGLVVSVLSLVGFLGGGALG
                     MAVLPGWFDGWAWAGEHPFESRALLIGGVFFLAALGQGIAVHVARRMRRHLHAGPIVA
                     ADAVLGGIASLIAVAVLVWFVAAGLRGSTASIVARTIGESRVLAVVDALVPPQTGALF
                     AGFRQVLDRGGFPRVFEGLTTEPIIAVDPPSSAVLGGAGVVAAQASLVKVTGVAAACR
                     RGQEGSGWVLAPGRVVTNAHVVAGIEDVTVRVRGTGPALHGSVVVFDPQRDLAVISVP
                     GLNAPALPLGADLARGAEAVVAGFPLDGPLRLDAARVRQVINATGQDIYGQPGVTREI
                     YSLLTRVEPGNSGGPLLSSAGQVVGVVFAKSLDDDQTGYALTLAEAAPVLTAAAGAQG
                     RVDTGACSLG"
     assembly_gap    15339..15438
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15506..16417)
                     /locus_tag="PLK64_00090"
     CDS             complement(15506..16417)
                     /locus_tag="PLK64_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPV78166.1"
                     /translation="MSDAASVLLDGPWEHEYVAANGARFHVALAGDGPLVLLLHDFPQ
                     FWWAWRHQLVALADAGYRAAAVDLRGFGASDKPPRGYDTYTATADAAAVIRSLGEERA
                     VVVGAGLGGWTAWAMPNLQPAFTRGIGAVSMAHPAIMQRAARRDRAQRSALSFVGHLQ
                     WPFRPERSLIRDDTLVRRYLTSWAAPGGTFPSEEEISRYAAALALPFVAHSAAEYYRW
                     FGRNQVRRDGPLFQRRVGSPIQVPVLQVQGSADGCVLADATGGSQRYVSGPYTYQAIE
                     GAGHFLPEEAPTQVSRALIDWLATVPA"
     gene            complement(16459..16869)
                     /locus_tag="PLK64_00095"
     CDS             complement(16459..16869)
                     /locus_tag="PLK64_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HPV78167.1"
                     /translation="MATDAPPLISDAEPTLGQLVASASRDVSTILRSEIALAKTEITG
                     AAKFAGKGAGMFAGAAFFGLFGLGFLLTALAWGLVALGLATWLAFLIVAVLLFIGAAV
                     LALVGKKALAHADPTPKRAISEAQQTIATIKGQS"
     gene            complement(16939..18198)
                     /gene="nhaA"
                     /locus_tag="PLK64_00100"
     CDS             complement(16939..18198)
                     /gene="nhaA"
                     /locus_tag="PLK64_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HPV78168.1"
                     /translation="MTHPSFDVARRLLRRPSERESRHLGDILRTETVGGMLLLGATVV
                     ALAWANSPWHTAYTGLRDTRVGPSAPLHLDLTLGQWAADGLLALFFLVAGIELKREFV
                     SGDLADRTKAIVPVAAALAGVALPAILYAVTVLALGGDSTAMRGWAIPTATDIAFALA
                     VLAVVGTHLPPALRAFLLTLAVVDDLVAITIIALFFSSDVQPLMLAGALIPLAVWGWL
                     LRRGWTYPLLLAVPAVATWALVHEAGVHATVAGIVLGLTVPVARSNDADDSTEGHSLS
                     EQLEHGMRPYSAGLAVPLFAFFAAGVKITSDGLATAFRDPVTIGIIIGLVAGKSLGVF
                     SGTWLVARFTRAQLDEDLAWADIAGLSLLAGLGFTVSLLIGELAFGSGSARDDHVRVA
                     VLLASLVAAVLASIVLRRRDAHYRQLQ"
     gene            18367..19080
                     /locus_tag="PLK64_00105"
     CDS             18367..19080
                     /locus_tag="PLK64_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPV78169.1"
                     /translation="MTSSADPAALPQCAVAVDLVVLTVREGQLCVLTVRRGIPPFRGR
                     FALPGGFVLPDEGLEAAAYRELAEEAGLARGAVTLEQLRTFGELQRDPRGRVVSVAYL
                     ALGAGLPDPLGGSDASSAQFEPVSGYLAHPAGPTALAFDHPMILAAGVERARAKLEYT
                     GYGVAFCPPEFTVGELRGVYESVWDLAIDPANFHRKVTNAAGFLVPTGERTSRGGGRP
                     AALYRADPAAVLHPPILRG"
     gene            complement(19082..19660)
                     /locus_tag="PLK64_00110"
     CDS             complement(19082..19660)
                     /locus_tag="PLK64_00110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPV78170.1"
                     /translation="MPTPEAPRTGLIELRIVVASTRPRRLGPVIADWVRNQAPLDVFA
                     VEVIDLAELNLPLLDEVEMPSTGIYHHEHTLRWKGLVDAADAIVLVTPEYNAGYPAAL
                     KNAIDYLYAEWAGKPIALFAYGHSGGASASAQLSVVLSRVKATLVDGVGLRYRDHLDG
                     TVGPEATVRADAELLGATRRMYAALAAAARPN"
     gene            complement(19853..21823)
                     /gene="acs"
                     /locus_tag="PLK64_00115"
     CDS             complement(19853..21823)
                     /gene="acs"
                     /locus_tag="PLK64_00115"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HPV78171.1"
                     /translation="MSNETLANLLHEDRSFPPDPAFAAQANGTAELYAAADADYEGFW
                     GDQARRYVTWSKPFTEVLDWSNAPFAKWFADGELNAAYNCVDRHVEEGRGDKVALHWV
                     GEPGDTRDVTYADLHREVQQAANALIDLGLGKGDTVAIYLPMIPEAVVAMLACARIGV
                     PHSVVFGGFSAEALNTRIADAQAKVVITSDGGYRRGTPSALKPAVDAALDHADTSVQK
                     VLVVRRTGQDIAWNDGRDLWWHEAMAAANPVHVAQGMEAEHPLFILYTSGTTGKPKGI
                     FHTTGGYLTQAAYTNAVVHDVHPESDVYWCTADIGWVTGHSYIVYGPLALGATQVLYE
                     GTPDTPHQGRFWEIVQQYKVSILYTAPTAIRTFMKWGSDIPAKFDLSSIRMLGSVGEP
                     INPEAWMWYRNVIGGGHAPIVDTWWQTETGGIMISPLPGVTNLKPGSAQRPIPGIGAE
                     VVDDMGEPVPDGHGGYLVLTKPWPAMLRGLWGDPARYVETYWSRWPQYYFAGDGAKKD
                     EEGNIWLLGRVDDVMNVSGHRLSTTEIESALVSHPKVAEAAVVGANDEMTGQAVVAFV
                     ILRAGSSHADDDNTGIVEELRNHVAKEIGPIAKPKRILIVPELPKTRSGKIMRRLLRD
                     VAEGRQVGDATTLADTSVMDLIKTGLSSGSDG"
     gene            complement(21950..22252)
                     /locus_tag="PLK64_00120"
     CDS             complement(21950..22252)
                     /locus_tag="PLK64_00120"
                     /inference="COORDINATES: protein motif:HMM:NF023257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3349 domain-containing protein"
                     /protein_id="HPV78172.1"
                     /translation="MGGLKMQSLRGWLVEGRPRGVARADLLAPALLRCELTEAETSAV
                     VGALRVRAGHSVADPVPDDDIRRVVQALVYEGADEAEVYRVREAMRRAGDRVTSHT"
     gene            complement(22573..23604)
                     /gene="trpS"
                     /locus_tag="PLK64_00125"
     CDS             complement(22573..23604)
                     /gene="trpS"
                     /locus_tag="PLK64_00125"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPV78173.1"
                     /translation="MTTQPSGIPPRSRPRSLSGMQPTSDSLHIGNYVGALVNWVELQE
                     TYDAFYFVADLHALTVPTGPAVLRRRTQVTAAQFIAAGVDPERSAVFCQSHLPQHAEL
                     GWVMSCLTAMGEASRMTQFKDKTAKGQQGNVGLFTYPMLMAADILIYDADKVPVGEDQ
                     RQHLEITRDLALRFNQRYGQTLVVPEPHILKQTAKIMELTDPTSKMSGTNSPDAGVVL
                     LSDDPKRITKKIKSAVTDAEMEIRYDPEHKPGVSNLLVIQSALTGVPIPALEAGYAGQ
                     GYGALKTQVAEVVVAAAEPFQRRMTELLDDPAELDRVLARGAERAGAVSAATLARVKD
                     RMGLLPAAH"
     gene            complement(23862..24203)
                     /locus_tag="PLK64_00130"
     CDS             complement(23862..24203)
                     /locus_tag="PLK64_00130"
                     /inference="COORDINATES: protein motif:HMM:NF023257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3349 domain-containing protein"
                     /protein_id="HPV78174.1"
                     /translation="MTSSPSIAARILDWLRGGYPEGVPSGDYIALLGILHRRLTTAEV
                     QALAGDLAAEAADSPPLTAEDVRALIEDRIHEDADDESVARVSARLAAGGWPLAPLRD
                     GDSLPPAPRQP"
     gene            complement(24212..24508)
                     /locus_tag="PLK64_00135"
     CDS             complement(24212..24508)
                     /locus_tag="PLK64_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78175.1"
                     /translation="MNNIGLAFDGAWKVLVAGVLLGAGLPAIFALGIRALAYGAGGDA
                     EVDHEAGHPIGKVLAILCFALVVIGIALGITYIVATGFGKTLSFQNFYPTLVSK"
     gene            complement(24521..25747)
                     /locus_tag="PLK64_00140"
     CDS             complement(24521..25747)
                     /locus_tag="PLK64_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPV78176.1"
                     /translation="MTTATVILGLVVATALAFDFTNGFHDTGNAMATSIATGALRPKA
                     AVLLSAVLNLVGAFLSVQVALTVTNAVVKIQDKSGAPSAALLADGGYALLLIVLAGLC
                     GGIVWNLLTWLLGLPSSSSHALFGGLVGSAIAGLGLSGVNWVGDGSKLDGVVGKVIMP
                     ALLSPVIAVVVSTIGTWLIYKITAGVAQRFQEAGFRWGQIGSASLVSLAHGTNDAQKT
                     MGVITLALIGAGQWSDLTAVPLWVKVTCAVAIALGTYLGGWRIIRTLGKGLVEISSPQ
                     GMAAESSSAAVILASSQMGFALSTTHVATGSILGTGLGRRAEVRWGVAARMLVAWTIT
                     LPAAALVGAAMWFIGHLLGGLLGAVVVFALLVGLAAYMWVRSRRAPVDHTNVNDAWDT
                     DASKLSETLATSPARI"
     gene            complement(25998..27578)
                     /locus_tag="PLK64_00145"
     CDS             complement(25998..27578)
                     /locus_tag="PLK64_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HPV78177.1"
                     /translation="MPAATQVGNTTLNIAIFGLFVLITLGIVIRVSKQNTTAADYYAG
                     GRAFSGRQNGIAITGDYLSAASFLGIAGAIAINGYDGFLYSIGFLVAWLVALLLVAEL
                     LRNTGRFTMADVLSFRLRQRPVRTAAAVNTLAVSAFYLLAQMAGAGGLVALLLGITDR
                     TGQSIVIAIVGALMIVYVLIGGMKGTTWVQIIKAALLILGAGIMTIWVGVKYGFNISA
                     LFDDAIKQAGNAAILNPGLQYKNKIDFISLSIALVLGTAGLPHVLMRFYTVPTSKEAR
                     RSVVWAIWLIGLFYLFTLVLGYGAQALVGSAAIKAAPGGVNSAAPLLAYHLGGELLLG
                     VISAVAFATILAVVAGLTITASASFAHDIYAQVIKRGTVSPDDEVKVARRTAVVIGAL
                     AIVGGIFANGQNIAFLVALAFAVAASANLPTILYSLFWKNFTTRGALMSMYGGLASSL
                     LLIIFSPVVSGSATSMFPTASFAWFPLNNPGIISVPLSFFLGWLGSVTEKEHNAAKYA
                     EMEVRSLTGAGAEKAVSH"
     gene            complement(27590..27934)
                     /locus_tag="PLK64_00150"
     CDS             complement(27590..27934)
                     /locus_tag="PLK64_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HPV78178.1"
                     /translation="MSSHNDISEERAARYIAVQNSQEFAELRKTFRKFVFPMTGLFLA
                     WYFLYVILATYASDFMATKLFGNITVGLVLGLAQFATTFAITMIYARWADRVLDPAAA
                     KLADHMERGNLQ"
     gene            complement(28089..29645)
                     /locus_tag="PLK64_00155"
     CDS             complement(28089..29645)
                     /locus_tag="PLK64_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HPV78179.1"
                     /translation="MNTLASGWGLLAIILVCVATVAVGALGLRMSRTTSDFYVAGRVV
                     TAGWNASAIGGEYLSAASFLGVAGIVYSLGTHMLWLPVGYTLGYLILLVLVAAPLRRS
                     GAYTIPDFAEARLSSRRVRRIASVLVVGIGWLYLLPQFQGAGLALQTVTGASPAVGSL
                     IVAVVVLVSVAAGGMRSITLVQAMQYWLKLAAISIPCFVLAWLWFRGGQPLPSLTDPG
                     GPFAGALPAYPMREHPLYATYSTLLALCFGTMGLPHVLVRFYTNPDGRAARRTTVIVI
                     ALLGAFYLFPPVLGMLGRVYLPSLPAGTRADSVVLLLPSAVTPGTLGDLLSAILAGGA
                     FAAFLSTASGLTVSVAGVIDQDLLSGAMNRRLGGDLPRTHSFRLASLVAILIPYAASW
                     LAPGATLADTVGMAFAIAASTLGPLLILGVWWRRLSVTGAAAGLIVGGTLAMGATVAT
                     LFGVGRGTGGWLPVLLAQPAAWSMPIAFTTAVLVSLATPRSVPVNAARAMVRLHAPEE
                     VTARVRPPAG"
     gene            complement(29656..30084)
                     /locus_tag="PLK64_00160"
     CDS             complement(29656..30084)
                     /locus_tag="PLK64_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78180.1"
                     /translation="MTPTAGPPDPRGDRSLPEVGPAPKRVRVVSPRRDARRRKEKRPD
                     AVEFTEQTGLGEIYLTALLRAQLRLSLAILVAVGGILVGLPALFLLVPGAAAATLGPV
                     PVIWLVAGVAVYPIVLLAARLHVRMAERIERDFTDLMAGR"
     gene            complement(30089..30853)
                     /locus_tag="PLK64_00165"
     CDS             complement(30089..30853)
                     /locus_tag="PLK64_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPV78181.1"
                     /translation="MTTTTSSPPLRALLVDDEAPTLAELAWLLGQDERIGEIYTAGSG
                     TEALKALDDHPVDVIFTDISMPGLDGLALAKVLARFADRPQVVFVTAYDEHAVTAFEL
                     EVTDYVMKPVRADRLGEAVRRVVAQRAEKRVTVVPVPDAPADETIPVELGGVTTFVQR
                     SQILYAQAQGDYARLHTDRGSHLVRISLNALEERWGDHGFVRIHRSTLVSTAHITAVT
                     VQSGRCKVSLGDDIALEVSRRHTRALRDRLIRSAAR"
     gene            30947..31537
                     /locus_tag="PLK64_00170"
     CDS             30947..31537
                     /locus_tag="PLK64_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV78182.1"
                     /translation="MKRSYRQSERARQAEETGVRILQETRRLFEAQGYAGLTLASVAE
                     AAGVTTQTVIRRFGDKEGLTAAAGQRVAQEIYAQRDEAPAGDLAAIVRNLVEHYETRG
                     PVSLRLLADEHLSEAVASFTRAGRDFHHGWCERVFAPWLADLDGSTSRRRVAQLVAVC
                     DVRTWELLRHREGLTPAQTRTALLELLRPLTEGARQ"
     gene            31534..32778
                     /locus_tag="PLK64_00175"
     CDS             31534..32778
                     /locus_tag="PLK64_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPV78183.1"
                     /translation="MTAVLAYTSPAMGHLYPMMPLLLELRDRGLDVHVRTLDTLVDQA
                     RAAGLSAERIDARLRPIADKHPDWAATNAKAALERSATMFAERAVLDGPDFRRAIEEV
                     DPDLLIVDTNAWGALVVAEAQRRPWLHFSPYLVPLRSTGTPPFGPGLPRWDNPVGRVR
                     DAVVGRLVFGAVERSIIPRINALRTPAGLAPVRSFDDLVLAAPLVAICSATPFDYPHP
                     DWPSHVISIGAMTWEPPTAPPAWLADLPDPLTLVTTSSEYRADEALARVAVAALASEP
                     GSLVVTMPAGVPDDLRDLPPNVRVEQFVPHAPLVARAAVAVTHGGMGATQKALAGGIP
                     VCVVPYGRDQLETAARVVHADAGTQLAPKKLTPAALRAAVRAAAGKRAGAAACADGFR
                     AAGGARAAADALLAQLASPSRR"
     gene            complement(32911..34110)
                     /locus_tag="PLK64_00180"
     CDS             complement(32911..34110)
                     /locus_tag="PLK64_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPV78184.1"
                     /translation="MPELPTPGEVTLVTVGLLAALLLGAVVMRLVGRRRFVDPVDRAT
                     YETLHTASLATRSLRDGLTPEGATRATRHLRSMLGSTAVAIVDTATTLAWDGAGSHHR
                     GDVRAHAEPVLSSGRTLVLTSDQITCSDPDCSIRSGIIAPIVADDRVVAALAAYGRTT
                     STGLVRATEELAGWISTQIELAELGRQRTRVMEAELRALRAQISPHFIYNCLGAIASF
                     VRTDPDRARELLLEFADFTRYALRRGGAYTTLRDELQNIERYLALENARFGDRLVVSL
                     TIAPEVLAVSVPFLAIQPLVENAVRHGIEHKAGIGHVTITASDVGANAEITIEDDGAG
                     SDPDTIRAVLAGESSRDSVGLGNVDARLRQAFGDEYGLVVETAPGAGTKVTFRVPKFA
                     PGVHSGS"
     gene            34154..34996
                     /locus_tag="PLK64_00185"
     CDS             34154..34996
                     /locus_tag="PLK64_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPV78185.1"
                     /translation="MSDARAAVLTLLDLEGVREAVDAARDACTQLRWHEALRRRIPEA
                     SAESRVRGSRASAFLDGAELPVDLVRDLVRGARPWPEHLDPVEEVLRGAIQATAETEH
                     IRGLLVRSPSQALARLHVAAASGLLPVEQVGRPRLGEETSREFVDLGMPPAPAELPAR
                     LAGVAELIGLAGEVPVVLAAALVHAELAVVRPFVRGNGLVARAAERALVAAAGLDPTG
                     VAVPEAGWLAGGGTAYLGALTAYASGTPQGVALWLRQAAQAMTQAAAEGARVADAVRV
                     GRLT"
BASE COUNT         5247 a        11724 c        13037 g         5387 t
ORIGIN      
        1 cgtagttgtt gtcgatgagg tacttgcgga tcttctgctc cgcgccccct cgatagagca
       61 ccccgggaaa ctcgacgata gccgcggtgc cgttgaccgc tagccagtgc agcatgtgca
      121 tcgtgaaagc gaggtcggct ttgctcttgg gtgcgaggac gccggcgggg gcgaaccggg
      181 ggtcgttgat gagcagctgg tcgtccgcgc ctgcccacct cgtcgaatag ggcgggttgg
      241 acacgatcgc ctcgaacggc tcgtcgtccc agtgggctgg atcgatgagg gtgtcgccgt
      301 gggcgatatc gaactgctcg aagttgacgt cgtgcaggaa catgttgatg cgggcaaggt
      361 tgtaggtcgt caggttgatc tcctgaccga agaacccctg ccgcacgttc tccttgccga
      421 gcaccttggc gaacttcagc aggagacttc ccgagccaca cgccgggtcg tagaccttgt
      481 tgaccgacgt cttgccgacg accgtgatcc gggccagcag ctcgctgacc tcctgcgggg
      541 tgaagaactc cccgcccgac ttgccggcgc taccggcata catggtcatg aggtactcgt
      601 aggcgtcgcc gaaggcgtcg atggtgttgt cggcgaaggt gcccagggcg aggtcgccga
      661 tggcgttgag gagcttgacg agcttctcgt tgcggcgggc cacggtgggg ccgagcttgg
      721 cgctgttgac gtcgaggtcg tcgaagagcc ccttgaggtc gccctcgctg tcggtaccga
      781 tggccgagcc ctcgatgttg gcgaagaccc gctcgagcgt ctcgttgaga ttcgggtcgt
      841 gcggcgcgcg cgcccgcacg ttcgcgaaga gctcgctggg caggatgtag taacccttct
      901 cttcgaccgt gacctcacgc ccgaactcgg cctgctcgtc ggagaagtcg gcgtagtcga
      961 agtcgccgac gctggcagcg cgctcgaggc cattgatgta tgccgtgagg tgctcggaga
     1021 tgaacctgta gaagagcaag ccgaggacgt agctcttgaa gtcccagccg tcgacactcc
     1081 cgcgaaggtc gttggcgatc cgccagatcg tgcggtgcaa ctcggcgcgc tgctgctcct
     1141 tggtcatggc gcgatcacat gggtaggtcg tggagggcat ggcatgggct gatcctgcct
     1201 catcgggcgg gctccgttcg tgcggcctcg ccgtgtgcac cgacgccgtg aagttcgtgc
     1261 cgaatcctcg cttcggaccc cgatggggtt acttgcacac ggccaccgag cccggcggcg
     1321 accgccgacg ctcccggccg cgacgtgccc gggatggctt actatgcccc agtgatgccc
     1381 gggagccagg caagcaaaag gcccctgact gcgtttccgc aggtcagggg cccattctcg
     1441 ctgtcggggt gacaggattt gaacctgcgg cctcttcgtc ccgaacgaag cgcgctacca
     1501 agctgcgcca caccccgtgg cccgcgcgac cctggtgggg gtgttacggc acaaggacag
     1561 aggatagccg acccgtgcgc ctggcaccga atcgggtcgg tgggcgggcg gatcgcagac
     1621 cggtgggcgg gcggcatacg ggatgacggg ccaaccacgc gctccctcgc gccgaccacc
     1681 ggtcgcccgc cgagaggtgg gaatcagccg gtatgccgtc ccgcgaggcg gcagaatgcc
     1741 acccttcgca gggtgggcac cgccacaggc caaaacccct aggagtcgcg ggggaccagg
     1801 gtgagcaggc ttgcttcggg gcggcaggcg aagcgcacgg gcgccatcgg cgaggtcccg
     1861 aggccggccg acacctcgag gtatgccgcc ccctgcggcg cctgcgcgga cggcgcccca
     1921 cctgcgccgg gccaccaccg cgaaacgccc ttggcgcgcc gcgtgtccag gtcgcagttg
     1981 gtcacgaggg cgccccagcc gggcaggcag agctgcccgc cgtgggtgtg gccggcgatg
     2041 accaacccgg ccccgtcagc agtcatcgca tcgagcaccc gctgataggg cgcatgggtc
     2101 acgcccacgg tcagcgcgac atcgtccgcc gcggcccccg caacaacgtc ataccggtcg
     2161 tagtccaggt gcgggtcgtc gacccccacg aactccaggg gcaccccaga caccgccagg
     2221 cgcgtccgcc ggttggtcag gtcgtcccac ccacccgcag ccaggcctgc caccagacgc
     2281 tccgtcggca ggcgcgacgc ctctcccagc ccccgccggt ggttgcccgt gagatacagc
     2341 gccgggttct tcatccgtgg cgcgaagtag tcgttggacc ccaggacgaa caccccaggc
     2401 accccgagca gcggctcgaa cgcccgcagc aacggctcga tcgaccccgg gttggcgatg
     2461 ttgtcgcccg tgttgacgac aaggtccggc tcgagccccg ccagcgctcg gacccactcg
     2521 atcttgcgcg cctgggcggg catgaggtgc aagtcggtca ggtgcagcac ccgcagactc
     2581 gcgctcccgg gcggcaacac cggcagggta tgccgccgca agacgaagag gttgcgctcc
     2641 accaggctcg cgtagcccac tcccaggacg cccacggcgg tggctcccac agcggcacga
     2701 gcaagcggat gcatccgacc agtctcccac ggcagcaccc accgggccga aacggaagcg
     2761 acggggcggc atacaggctg ggagactgga cccatgagca ccctcaagga gaccctgcac
     2821 gccgacctca ccgaggcgat ccgcagccgc aacgcggtcg tcgccgcgac cctgcggatg
     2881 gcgttgacgg cgatctccac cgcggagacg gccggcacga cccaccgcga gctcagcgac
     2941 gacgaggtcc tcaaggtcct ggtcaaggag ggcaagaaac ggcgcgaggc gtccgcggca
     3001 tacaccggcg cggggcgacc cgagttggcg gcgcgcgagg acgcggaatt cgcggtgctt
     3061 gagggctacc tgccgacgca actcaccgac gaggagatcg cggcgctcgt cgcccaagcg
     3121 gtggctcaga ccggagcgac gggtatgccg caactcggca tggtcatgaa ggctgtccag
     3181 ccgctcgtgg ccggacgcgc ggacggtggt cgggtcgctg ctgcggtccg ggtggccctg
     3241 acggcgggct aacctggcgg cgctggctcc ggcggtgcgg tcggcgccgg cgccgggccc
     3301 tgggagacga ggatcttgac gaccgatccg cgcgtggcct ggttcttggg agtagttccg
     3361 acgacggtgc cgactcggta gttcgagtag accttgacga ccgacggcac gaaaccggac
     3421 tgcaggagca gcgccttggc gtcgtcgagg gagcgacgcg cgatgtcggg gatgtcgact
     3481 ttctcgccgt tgacgatctg ggtgcccggg ctgatgaacg gcaccttggg ctggccttcg
     3541 agggcccggt ccatgatcga cttccagacc ggggcggcga ttgtcatacc gaacagggcg
     3601 gcataccgac cggccgagca gcccttcgtc tcggtcatct ggcgcatgca caggtcggtg
     3661 acgcgccggg cgttgttctt gtcgtccggc gtccccaccc acacggccgt cgtcagctgc
     3721 ggggtgtaac cgacgaacca tgtctcgttg tggttgtccg tcgttccggt cttgccggcc
     3781 gccggccgcc cgcccaccag ccccgcgttg tcggccgtgc cgccgttgcg caacacgcct
     3841 tccaagaggc tggtgacgcc gcgcgcgacc tcgggatcga cgcgcttttc gcacgcgctc
     3901 cccaaagcgg gcagcggcaa cgccttgccg tccttgacga tcgacatcac cgggatggga
     3961 gggcagtaga caccctcgtt ggcgatggtc tgataggtgc tggccatcgt catgggggac
     4021 gccgaccagg tgccgaggat gatcgccgac gggtagggag ggatctcctt gccgttgccc
     4081 tggtgcagcc ccatccgcca tccggtgtcg cggatcttgc acgggtcgcc accgagttgg
     4141 atcgccatcc cgacgaacgc ggtgttgatc gacttcgcgg tggcctcggc caggctgatg
     4201 tccccttcgg ggaagttgat gtcgttacgg acgttccacc cgccgccgcc gaggccgcac
     4261 gctccgtgga tctccttgct ggtgtagtcg gctcccttgc tcgggctggc cgccttggca
     4321 aacacgctgc tggtcgtggg ccagccggct tcaagggccg tcacgagcgt gaagatcttg
     4381 gctgtcgacc cgaaggagaa gccgatgctg ccgccgtagc gggagtcgac cgaccagttg
     4441 accgcggtct cgccccggtt ggcgggtttg acgctgtagg tcgtgttttg tccgatggct
     4501 ttcacggcgc cggtcttggt gtcgagaatg gcagcggcag acccgatctc ctcgtcgttg
     4561 ccgatgggca ccttgcgagt gatttcctcg ttggctacgc gggccatcgc cggatcgagg
     4621 gtggtctgga tggtcagacc gccccggtag atcgcctgga cgcgctgggc ctcaccccgc
     4681 cccaacaccg cgtcgaggga ggggtcttgt ttgagccagg ccaggacgta gtcacagaag
     4741 taggggtagt cggaggactg gcaggagttc ttggccgggg tgactttgag catggccgcc
     4801 aggggcacgg cgcgcgcggc cttccactcg gcgtccttga tgagcccgag atccttcatc
     4861 cggtccagga cgacgttgcg ccgagccagg gacttctcgg ggaagttgac cgggtcgctc
     4921 gtgccggggt tctggacgat cccggcgagc aatgccgctt cctgcaggtt gagggtcttg
     4981 gcgccgtgcc cgaagtagtg ctgggaggcc gcctcgacgc cgtaggcccg gtcaccgtag
     5041 tagacgaggt tgaggtagcc ctcgaggatc tggtccttgg tgagggtctt ctccaactgg
     5101 atggcgtact tgagttcctg gatcttgcgg ccgtagttcc gcgcggtggc ggccgccgcc
     5161 ccagccttgt cgcccgctcg cagggcgttc tcctgcagcg tgatcttgac gtactgctgg
     5221 gtgagggtcg acccaccgcc ggtcaactca ccggtggcct ggttggagat gaaggcgcgc
     5281 aggatgcctt gcaggtcggc gccgccgtgc tcgtagaagc ggtggtcctc gatggcgacc
     5341 tgggccgtcc gcatgaccga cgcgatctca ttcagcggca cgatgacgcg gttctcgtcg
     5401 tacggagtcg cgatcttcgt gccgtcggcg gcgaggatga ccgactgttc agccagcggc
     5461 gaggttgtga agttgtccgg gagagagtcg aagaggtcaa cgacggtgcg gaccgacccc
     5521 ccgatcgcac cgaccgcggg caagaacagc cccgccgaca gaaagccggt gatgactgcg
     5581 gccgcgagga acgccccgag gaggctgacc acgccgccca cgttcgtcgt tcgtccagac
     5641 atgtccgcaa gggtaacgcc ccagcctgta tcaggggtcc gcgagcgctc tccttcaggt
     5701 atgccgcgcg cccgacgacg agcgaatgtc gccgggcagc gcggcccacg acccttcggc
     5761 gacgagccgt tggtccggtc ctcgctccag cggaggacta gtcattcttc gaagtcaacc
     5821 atcccaacgg gtcatggtgg ccgagttgga gcctccccta acgtgccaac gagggacgaa
     5881 tcggggcggt ttgtcctcaa cgggctgcgg gaaaggtagg aggaatgtcc actcccatcc
     5941 ttcgtggcgc tttgcgccag gactgggcgg gcctcggggc gtgcgtgtca ggggacccgg
     6001 acgatctttt cgtcaaaggt gcagcgcaac agcaggccaa agaggtctgc cagcgctgcc
     6061 ccgtcatcgc cgaatgcctc gctgaggcac tcgacaaccg caccgagttc ggtgtgtggg
     6121 gtgggatgac cgagcgagag cgacgcgcgc tgctgcgacg caaccccacg gtgacctctt
     6181 ggtgggcgat gttcgcccac gccggtgccg gccgcgccga cgtcgccagc tgacggccag
     6241 gttcgccgtc agggtgccgt tcgcgcactc acggcagtcg agcactcacg gtaggccggc
     6301 caatcggcgg ccgacctcgc gtagcccgtc ggcatcgtgg acgtcttgcg ccaacgatgc
     6361 cacttcgacc accgggatcg acgggtggcc ggcggtgaac cggcgcacca ggttgcgatg
     6421 ccgcgccgcc aactccgaga cctcggcatg caggctcaag gcagccgctg cggccggccg
     6481 gtcagcctcg gccaactgct cggcagcggc ccgagcccgg gccgcggtga gccccggtgc
     6541 ggcagagggc tgcatccggt tgacgacgac tccggcgagc ggcataccct cctgctccag
     6601 ccgggccacg aagtaactgg cctcgcgcag cgcgtcccgc tccggggcgg cgacgacgat
     6661 gaactcggtc ccgccctcct tgagcaacgc gtaggtgagg tcggcgcgct cgcggaaccc
     6721 gccgaacatc gtgtcgagcg cgttgacgaa ggtctgcacg tcgcgcagca tctccccgcc
     6781 gagcaccgtc gtcagcgtct tggacaccag cccgatgccg acgttgacca ggcgcaaccc
     6841 ggcccgcccg cccatcttcg cgggcgcggc gatgagccgg atgaagcgcc cgtcgaggaa
     6901 cgatccgagc cgcttggggg cgtcgaggaa gtccagcgcc gaccgggacg ggggagtgtc
     6961 gacgacgatg aggtcccacg tgccctcacg gtcggcgcgg gcccgcagct gcccgagctt
     7021 ctccatcgcc atgtattcct gggtccccgc gaacgacgac gacaccgcct gatagaaggg
     7081 gttggcgagg atctgctccg cggcctcgcg ggtcgaatgg gcgagcacga cctcgtcgaa
     7141 ggtccgcttc atgtcgagca tcatcgcgtc gaggcgccca cccggcaccg catcgtcggc
     7201 cagcccgctg accggcctcg gcacgttgtc gagctcggtg agcccgagcg actgggccag
     7261 cctccgcgcc gggtcgatgg tgagcacgac gacctcgcgg cccgcctcgg cggcgcggag
     7321 ggcgagcgct gccgcggtcg tcgtcttgcc gacaccgccc gagccacaac acacgacgat
     7381 ccgggtatgc cggtcggcca gcatcgcgtc gatgtccagg acgggggtgc gggccatcag
     7441 ggcaccatcc cttgctcggc gagcagggcg gcgatctcgg tcaccgcgtc gaggtcgatc
     7501 ccgtcgatga ggaacggcac ggtgaccagc ggttggccga gttgttcgag caccgtgagc
     7561 tgctcgacct cgagggcgac ccgctcggta tgccggtgaa cctcgtcgag cagtccctcg
     7621 acggtggctc tgggcaggtc cagcccgacc gcctcgaggt cggcgctcaa cttggctgcc
     7681 gccgtcgggt cggagttcag cggcgcgtcg agcccgccga aagcgccaag cgcgtcgagg
     7741 tcctcgcgcg cctgggcttg gttgatgacg accgcgccga ccggcatacc gagctcgcgc
     7801 aactcggcca cggcgtcgac ggtctcttgc acgggcatct cctccaggag ggtgaccacg
     7861 tgcacgcggg tcgcgtcgga gtgcagcaac tcggtgatcg agccggcctg cgagtggatc
     7921 gggcccatcc gcgccaaccc ggcgacctcg gccccgacgg agaggaaccg ggggatccgc
     7981 ccggtcgggg gcgcgtccag gacgatggcg tcgtaggcga gctcctcggg cgggctgccg
     8041 cggtggtgct tggcgtcgtg acggcggcgg ctggcttcgt agacccggcc gatgagcaac
     8101 aggtcgcgca ccccgggggc gatcgtcgtc gcgaagtcga tggccccgaa acgctccagc
     8161 accccaccgg cgcgaccgag tttgtagaac aactgcaggt actccagcag cgccgtcttg
     8221 gcgtcgacag ccagcccgac gacctcgccg ccctgcgcgc cgcccgcgac gcggacctcg
     8281 gtcgtcggca acggcgggac gtcgaaggtc tggctgatgc cttggcggcc ctcgacctcg
     8341 gcgagcagga cccgcaggcc ctcgttggcc agcgcgacgg ccagggccgc ggcgaccgtc
     8401 gtcttgccgg tgccgccctt gccggtgacc acgtgcagcc gcacaccggc aaaaccgtcg
     8461 gcgcgcaacg ggtcgggggc gggcggcata cggccaaatg tatgccgtcg cgccgctgcc
     8521 cggctgccgt cctgcccgca ggccgcctgt gggcggtgcg cgccgttaga gtgaccggca
     8581 tgacttcgtg ggagtacgca acggtgccgc tcatcgtcca cgcgacgaag gcgatcctgg
     8641 acaactgggg ccgcgacggc tgggagctcg tccaggtggt gcccgggccg gacggcaatg
     8701 gcctggtggc ctacctcaag cggccgctgg ctggtggcca ggcatgagcg ccgtcgagga
     8761 gcgccttgcg gcgatgggct attcggtccc tgacgtcgtg ccgccggtcg cggcctacgt
     8821 ccccgcgctg cgccaaggcg actgggtgtt cgtgtcgggg caactgccga tgcgggacgg
     8881 cgcgatgcct gccaccggga aggtcggtga cggtcccgga caggtgagcc cggaagcggc
     8941 caagcagctc gcggcgttct gcgcgctcaa cggcatcgcc gcggtgaagt cggtcatcgg
     9001 tgacctcgac aaggtcgtgc ggatcgtcaa ggtcgtcggc ttcgtcgccg ccgacccgtc
     9061 gttcaccgga gtgcctggag tcgtcaacgg ggcgagcgag ttgctcggcg ctgtcttcgg
     9121 cgacgccggc gtccatgccc gatcggcggt gggtgtgagc gtgctgccgc tcgatgcccc
     9181 cgtcgaggtc gagctcatcg tcgccgtccg cgactgacgc gggcgccggg catgatccgc
     9241 gacttctcca ccagcaacgc tcccgcgttg catgcgacag ccttggcgtg gctgtccggt
     9301 gaacacggcg agcctgcgcc gctgcgcccg gccgccaccg tgatgatcgt ccgcgacgcc
     9361 tcggatgacg agtctggcaa ctctggcggc agccccgtcg aggtgttcct gctgcgccgg
     9421 gtcgcgtcga tggccttcgc cccgagcacg atcgtcttcc ccggcggggg agtcgacccc
     9481 cgtgacagcg cggagttgcc gtgggccggc cccggcgtcc tggaatggtc ggtccgcctc
     9541 ggcgccgatg aggaggccac gcgcaaactc gtggttgccg cggtgcggga agtcttcgag
     9601 gagtgcgggg tgctgttcgc cagcgccgac gaggacgggc ctctggtcga ggtgtccagc
     9661 ccgcagtggc agcaggtccg cgccggcctg gtcgcccgcg agttgtcgtt gagtgacgtg
     9721 ctgcgtgagc acgggttgtg tctgcgcacc gatctcctgc gcgcccacgc ccactgggtg
     9781 accccagtct gcgagccgcg acgattcgac acgcggttct tcgtggcgct catgccggcg
     9841 ggccgggcca tcgacggtgc gacgagcgag gccgaccacg ccggctggtt cgacccggca
     9901 tctgtcctgg ccgactatgc cgccggttcc gcggtggtgc tgccgccgac tctggtctgc
     9961 ctggaggacc tcgcagcctc cccgtcggct gccgagttct tcgcgcagga gccggtgatc
    10021 cgccggatca tgcctgagtt ggtcgtcaac gacgccggcg aggccgtgat gcgcaccgat
    10081 ttcgcgggca ccaaggctcc gcgcgggtga ctctcacgct cccgccagtg ccacccacct
    10141 gggcatccgg gcaggtgacg gagcgggcct actgcgtctt agctcccaac cccggggtga
    10201 tgaccctcga cggcaccaac acatgggtgc tgcgcgagcc gggagcgcaa gagtgcatcg
    10261 tcgtcgaccc gggcccgctg gacgaggccc atctgacgcg ggttctcgac gtcgtccggc
    10321 gcgacggggg ccgagtcggg ctgaccctgc tcacccacga ccattccgac cacgccgatg
    10381 ccgccgagcg cttcgcccat ctgtccggag cgccggtgcg agccatccgg gccatcggca
    10441 ccggccacga cgatctggcc gacggcgact cggtgagcgt cggcgggctg gacctggtgg
    10501 tcgtcaccac gccggggcat acgagtgatt cggtgagctt cctcttgccg gccgagtgcg
    10561 ccgtcctcac gggcgacacg atcctcgggc gcggcacgac cgtcgtggcc tggcccgatg
    10621 gtgagctcgc ggcatacctc gcctcgctgg agcggctgga agccctctgt gggtccggcg
    10681 cggtggcgcg gatcctgccc ggccacggcc cgcacctgcc cgatgcggga gcggtgctgc
    10741 gctactacct cgagcaccgc gcgcagcgcc tggagcaggt gcgtggggcg gtcgccgccg
    10801 gtgcgcgcac tccgtgggag gtcgtcgaga cggtgtatgc cgatgtgccc cgggccgtct
    10861 ggggcgctgc ggaatggagc gtgcgggctc aactggccta tctcgccgag cgcggcgagg
    10921 aaggccagct gggctagggc aggccgatcg ccagggtgtg gccctcgacg aacgaggcct
    10981 cgaccgcggt gatcgtcagc ttctggtcgt cggtgtaacc caaggtctcg ccgggcatga
    11041 gcaccgcacc cgattcgacg acgtagctgg cgatgttggc cagcaggtcc atgacggcgc
    11101 tcggcgccgc ggtggaccca cggacctcga gctcctcgtg cccgaacact cgcagccccc
    11161 gcgtggagcc gcaggacacg ccctcgctct cccaggcggc ccaggtgaac caggccacct
    11221 cccagggcag gccgtccccg gcgcccgcga ccgcgtcgat gtagaaggcc ggctcgtaga
    11281 ccacgccctg gtcacccagg tagaccccga cggtggcctc gtgcccgagc agcgcggcga
    11341 cgaggtgcgc gaaggcggca taccgctggg tatgccgttc gctcgggtcc gcgccgtctg
    11401 cgtccccgac gccgtcggct cgctgatcag acacgctgac gatgaggtgt gcccggtggc
    11461 gggccactgc agccgcggca tcgggccaga aggcgttgtt ggcggcactg gcctcggcct
    11521 cgccgtcggg gacgggagcg ctgatctcca ggacgaagcc ggtcgtgccg cccagggtga
    11581 tctcgaccgg cccggaagtc tcggtggggt cggtggcctc ggcgctggac ggcgagtgcg
    11641 ccatcggcgt ggcggatgca cccgggacgg tggcgacgag cgccacgatc tcatcgacgc
    11701 caatcgtccg ctccagaagc acaaacgcga gcaactgagc cgcgggctcg gggtggcgga
    11761 aactgcgccc aggaatcggc aacgaagaac ggatcttgtc gatcaagccc atgacaggcc
    11821 ccctccgcgt cgggtggccg ggcagctgcc cgcccggccg ttcgggtcag cgcgcgcgtc
    11881 gcttgagtcg ctcgatatcc agcagcagca ccgcgcgggc ttccaacttg agccaaccac
    11941 gcgtggcgaa gtcggccagc gccttgttga cggtctcgcg ggatgccccg acgagctggg
    12001 ccaactcctc ttgggtgagg tcgtgggcga ccatgatgcc gtcttcgacc gggcgaccga
    12061 accggttggc caggtcaagc agggccttgg cgacccggcc gggcacgtcg gtgaagacga
    12121 ggtccgcgag gtgctcgttg gtgcgacgca accgcgcggc gagtgcggcc atgagggctt
    12181 tggcgacttc gggacggccg gtgagcagct ccgtcagtga gtcgttgccg agccccatga
    12241 gctgggtctc ggcgacggcg gtggccgtca tcgtccgggg cccggggtcg aacagggaca
    12301 gctcgccgaa catctcgccc ggcccgagga tcgcaatgag gttctcgcgg ccgtcggcgc
    12361 tggtgcggcc gagtttgacc ttgccctcgc cgatgacata gagggtgtcg cctgggtcgc
    12421 cttcccgaaa gaggatgtcg cctcgttcga gccgcgaaga gttcatctgc gcgagcagcg
    12481 cttgaccggc ctcgtcgtcg agggccgcga acaacggcgc ccggcgcacc acatcgtgat
    12541 ccacgtcgca cagtgtgcca tgccgggtcg tcaactgtcc gggaggttgg tcccgtatgc
    12601 cgccctccgg actctgctgt tcagcgcgcc gggatgatcg ctctgcccag gcaggcggca
    12661 taccctgtcc ctcgtgcccg cagaccgtgc tgcccccgct tccgagacgc ccgtggcact
    12721 gacccgacgg gctcggcgga tctatcgcgc gctgctggag cggtatccct acgcccactg
    12781 cgagctcgac ttcacgacgc cgctggagtt gctcgtcgcg acgatcctct cggcgcagac
    12841 gaccgacgtg cgggtcaacc aggtgacccc gatcctgttc gcgcgctacc ggacggccgc
    12901 cgagtatgcc gccgccgacc gggccgacct cgaagccctc ctcgggccgc tgggcttctt
    12961 tcgggccaag accgagtcgc tgctcaagct gggtgcggcg ctcgtcgaac gcttcgacgg
    13021 ggaggtgccc ggccgtctcg atgacctggt gaccttgccg ggcgtggggc ggaagaccgc
    13081 aaacgtggtg ctaggcaacg cttttggcgt ccccgggatc accgtcgata ctcactttgg
    13141 gcggctcgtg cgccgcttcg ggtggacgac ggaggaggac ccggtcaagg tcgagcacgc
    13201 cgtcggggcg ctgttccccc gcaaggactg gacgatgctc agccacgtcg tcatcttcca
    13261 tggccggcgc acctgccatg ctcgcaagcc cgcgtgcggg gcctgcccgg tcgcgcagtg
    13321 gtgtcctgcc tacggcaccg gcgagaccga ccccgagaag gccgcccggc tgctcaccta
    13381 cgagttggcg ccgacatcgt gagttccgac gtctcgcggg tcgcccacgg cacccccctc
    13441 gtcccgctgg ttcccctggg cgccggtatg ccgcgtccgc ggcccgcgtg ggtcgacggg
    13501 atcctcgagg gggcccgcgc cgagcccgag tggttcaccc ggtatctgcc accggacggt
    13561 gaccagcgtc agtcggccgt cctcatcctc ttcgggcctg gcccccaggg cgaggactcg
    13621 ctgctgttca tcgagcgggc ccacaccttg cgatcccacg ccgcgcagat ctccttcccc
    13681 ggcggcggag tggaccccga ggacgacaac gtggtggctg ctgccctgcg ggagtcgttc
    13741 gaggaggtcg ggctggaccc gacgggtgtg gacatcgttg gcacgttgcc cgagttgttc
    13801 ctaccggtct ccgcctacac tgtgcacccg gtgctggcct ggtggaaaga cccgtcgccc
    13861 gtttacgtcg gacaccccga tgaggtggct caggtgctca gcgtcccggt gagttacctt
    13921 gcggacccgg ccaaccggca tacagtctcg cacccctcgg gctatcgcgg accggcgtgg
    13981 gacctcggtg acgacctgct gctgtggggg ttcaccgggg gcatcgtcaa caagattctc
    14041 gagttggcgg gcttcgcccg cgagtgggac agcgatcacc tggtgccgtt gccggagcgc
    14101 ttcatgcgga ggtcctcgtg accggcggga cgctcctcga catcgtgctg atcgtcctgc
    14161 tcgggtcgta tgccgtgtcc ggcttcggtc agggactcgt cgtgtcggtg ttgtcgctcg
    14221 tggggttcct cgggggcggg gcgctgggca tggcggtgtt gcccgggtgg ttcgacgggt
    14281 gggcctgggc cggtgaacat cccttcgaat cgcgggccct gctcatcggc ggggtcttct
    14341 tcctcgcggc gctgggtcag ggcatcgccg ttcacgtcgc gcggcggatg cgccgacatc
    14401 tgcatgccgg accgatcgtc gcggccgacg cggtgctggg cggcatcgcc agcctgatcg
    14461 ccgtcgctgt cctcgtgtgg ttcgtcgcgg cgggcctgcg cggaagcacg gcgtcgatcg
    14521 tcgctcggac catcggcgag tcccgggtgc tcgcggtggt cgatgcgctc gtcccgcccc
    14581 agacgggtgc gctcttcgcg gggttccgcc aagtgctcga ccgaggcggc ttcccacggg
    14641 tcttcgaagg tctgacgacc gagccgatca tcgccgtcga ccccccgtca tcggccgtcc
    14701 tcggcggggc gggagtcgtg gcggcccagg cctcgctggt caaggtgacc ggagtcgccg
    14761 cggcctgtcg gcgcggccag gagggcagcg gctgggtcct tgccccgggt cgggtcgtca
    14821 ccaacgcgca tgtcgtggcg ggcatcgagg acgtcaccgt ccgggtcagg ggaaccgggc
    14881 cggcgttgca tggctcggtc gtcgtcttcg acccccagcg cgatctggcc gtcatctcgg
    14941 tgcccggcct gaacgccccg gcgctgccgc tcggcgccga tctggcgcgt ggggccgaag
    15001 cggtcgtcgc cggtttcccg ctcgacgggc cgttgcgact ggacgccgct cgggtccggc
    15061 aggtcatcaa tgcgaccggt caggacatct acggacagcc gggggtcacc cgcgagatct
    15121 actcgcttct gactcgggtc gagccgggca acagcggcgg cccactgctg tccagcgccg
    15181 gccaggtcgt cggcgtcgtc ttcgccaagt cgctcgatga cgaccagacc gggtatgcgc
    15241 tcaccctcgc ggaagccgcg ccggtgttga ccgcggccgc gggggcgcag ggccgggtcg
    15301 acacgggagc ctgctccctg gggtgaccag tttcggccnn nnnnnnnnnn nnnnnnnnnn
    15361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15421 nnnnnnnnnn nnnnnnnngg cgcagggccg ggtcgacacg ggagcctgct ccctggggtg
    15481 accagtttcg gccgggcggg ccagatcagg ccgggacggt ggcgagccag tcgatgaggg
    15541 cccggctgac ctgcgtcggg gcttcctcgg ggaggaagtg gccggcgccc tcgatggctt
    15601 gataggtgta ggggccgctg acatagcgct gcgagccacc cgtggcgtcg gccaggacac
    15661 agccatcggc cgagccctgc acctgcagca ccggcacctg gatggggcta ccgacccggc
    15721 gttggaacag cgggccgtcg cgccgcacct ggttgcgtcc gaaccagcgg tagtactcgg
    15781 cggcggagtg ggcgacgaac gggagcgcca gcgccgcggc atacctgctg atctcctcct
    15841 ccgacgggaa ggtgccgccc ggggcggccc aagaggtgag gtagcggcgg accagggtgt
    15901 cgtcgcggat cagggaacgc tcggggcgga agggccactg caggtgaccg acgaagctca
    15961 gggccgagcg ttgggcgcgg tcgcggcggg ccgcccgttg catgatcgcc ggatgagcca
    16021 tcgacaccgc gccgatgccg cgggtgaacg cgggttgcag gttgggcatg gcccaggcgg
    16081 tccagccgcc gagcccggcg ccgacgacga cggcgcgttc ttcgccgagg ctgcggatca
    16141 ccgcagcggc atcggcggtc gccgtgtagg tgtcgtagcc tcgcggcggc ttgtctgagg
    16201 cgccgaaacc gcgcaggtcg acggctgctg ctcgatatcc cgcgtcggct agggcgacga
    16261 gttggtggcg ccaggcccac cagaactgcg gaaagtcgtg caggagcagc acgagcggac
    16321 cgtcgccagc gagggccacg tggaaacggg caccgttggc agcgacgtac tcgtgctccc
    16381 acgggccgtc caggaggacc gaggcggcat cggacatgtc attccttgag cggtgctgca
    16441 ggggcaccgc gggagaggtc aggactggcc cttgatcgtg gcgatcgtct gctgggcttc
    16501 ggagatggct cgcttggggg tcgggtcggc gtgggccagg gccttcttac ccacgagggc
    16561 caggaccgcc gcaccgatga acagcaggac cgcgacgatg aggaaggcca accacgtggc
    16621 cagcccgagc gcgaccagac cccacgcgag cgccgtgagc aggaagccca ggccgaagag
    16681 gccgaagaac gccgcccccg cgaacatccc ggcgcccttg cccgcaaact tggcggcgcc
    16741 ggtgatctcg gtcttggcga gcgcgatctc ggagcgcagg atcgtcgaga cgtcccggga
    16801 ggcggacgcg acgagctggc cgagcgtggg ctcggcatcg ctgatgagcg gtggcgcgtc
    16861 agtagccatg ggtgtcctcc tggggtggtg ctcctggagg cgagcctatc gccgcatgtc
    16921 ccactgcggt atgtcgcgct actgcagctg ccggtagtgg gcgtcgcgcc gacgcagcac
    16981 gatcgaggcc agcacggcgg cgacgagtga ggccaggagc acagcgacgc gcacgtggtc
    17041 gtctcgggcg ctgcccgagc caaacgccaa ctctccgatg agcagcgaca cggtgaaccc
    17101 gagtccggcc agcagcgaca ggccagcgat gtcggcccag gcgaggtcct cgtcgagttg
    17161 ggcgcgcgtg aaccgggcca ccaaccaggt gccgctgaag acgcccagcg acttgcccgc
    17221 gaccaggccg atgatgatgc cgatggtgac tgggtcgcgg aaggcggtgg cgagcccgtc
    17281 ggacgtgatc ttcacccccg ccgcgaagaa cgcgaacaac ggcacggcga ggccggcgga
    17341 gtagggccgc atcccgtgct cgagttgctc ggagagcgag tggccctccg tcgagtcgtc
    17401 ggcgtcgttc gaacgggcga cggggacggt cagaccgagc acgatgccgg ccaccgtggc
    17461 gtggacgccg gcctcgtgga ccagggccca cgtggcgacc gccggaacag ccagcagcag
    17521 cggatacgtc cacccacgcc gaagcagcca cccccagacc gcgaggggga tcagggcacc
    17581 cgcgagcatc aatggctgca cgtcggacga gaagaacagc gcgatgatgg tgatcgcgac
    17641 gaggtcgtcg acgacggcca gggtgagcag gaacgccctc agggctggcg gcagatgggt
    17701 cccgacgacg gccagcactg cgagggcgaa ggcgatgtcg gtcgcggtcg ggatcgccca
    17761 gccgcgcatc gcggtgctgt cgccgccgag cgcgaggacg gtgacggcat acaggatcgc
    17821 ggggagggcc acccctgcga gggcggcggc gacggggacg atcgccttgg tgcggtcggc
    17881 aaggtcaccg ctgacgaact cccgtttgag ctcgattccc gcgacgagga agaagagggc
    17941 gagcaggccg tcggccgccc actgcccgag ggtgagatcc aggtgcaggg gggcggaggg
    18001 accgacgcgg gtgtcccgca ggccggtgta tgccgtgtgc cacggcgagt tcgcccaggc
    18061 cagggccacg acggtggcac cgagcaggag catgccgccg acggtctcag tgcgcaggat
    18121 gtcgccgagg tgtcgggatt cccgttcgct gggccggcgt aggaggcggc gggcgacgtc
    18181 gaaggaagga tgtgtcatgg cgcctttcgg gtggtgcggg acccgatgag cggcgtcgaa
    18241 gcgacgcgcg gtgcagccga aggcgtgcga cacccgggtc gccgaccaga cttcccggca
    18301 cgccagagcg accagtctat caggagggtg tcgagattta gtcatcacga cgattaggat
    18361 caggtcatga cgagctcggc ggatccggcg gccttgccgc agtgcgcagt tgcagtggac
    18421 ctggtcgtcc tcaccgtgcg cgaaggccag ttgtgcgtgc tgacggtgcg acgcggcata
    18481 cctccgtttc gggggaggtt cgcgctgccg ggtggtttcg tcctgcccga cgaagggttg
    18541 gaggcggcgg cctaccggga gttggcggag gaggctggct tggcgcgggg ggcggtcacc
    18601 ttggagcagc tgcggacgtt cggggagctc cagcgtgacc cgcgaggacg ggtcgtgagc
    18661 gtcgcctatc tcgcactcgg cgcggggctg cccgacccgc tgggcggctc cgacgcatca
    18721 tccgctcagt tcgagccggt gagtgggtat ctcgcgcacc ccgcggggcc gacggcgctg
    18781 gcgttcgacc acccgatgat cctcgcggcc ggcgtcgaac gggcccgggc caagctcgaa
    18841 tacaccggtt atggggtggc gttctgtccg ccggagttca ccgtcggcga gttgcgcggg
    18901 gtgtacgagt cggtgtggga cctggcgatc gacccggcga acttccaccg gaaggtgacg
    18961 aacgcggccg gctttctcgt gccgaccggg gagcggacca gcaggggagg cggccgaccg
    19021 gcagcgctct atcgagccga cccggcagcg gtgctgcacc caccgatcct gcggggatag
    19081 gtcagttggg gcgagcggcg gcggccaggg cggcatacat gcggcgggtg gccccgagca
    19141 gctccgcgtc ggcgcgcacc gttgcctccg ggcctactgt gccgtcgagg tggtcgcggt
    19201 aacgcagccc gaccccgtcg acgagcgtgg ccttcaccct gctgagcacg acgctcagct
    19261 gcgcgctggc cgaggcgccg ccgctatggc cgtaggcgaa gagcgctatc ggcttgcccg
    19321 cccattcggc gtagaggtag tcgatggcgt tcttcagggc ggccggatag cccgcgttgt
    19381 actccggggt gaccaggacg atggcgtcgg ccgcgtcgac gaggcccttc cagcgcaggg
    19441 tgtgctcgtg gtggtagatg cccgtcgagg gcatctcgac ctcgtcgagc aggggcaggt
    19501 tgagctcggc caggtcgatg acctcgacgg cgaagacgtc gagcggggcc tggttgcgca
    19561 cccagtcggc gatgaccggg ccgaggcggc ggggccgggt gctcgcgacg acgatccgca
    19621 gctcgatgag tccggtgcgg ggcgcttcag gggtgggcat gaccgcatcc aagcagaacc
    19681 ccggcgagtg tgagggtctg gggtcgtttg gggggtcggg agccggaatt caccggcgag
    19741 tgtgaggatt tgggggaaca gcggggtgtg cggctgggcg atggggacac gccagagggg
    19801 gtatgccgtg ggcgcggcat accccctccg ggcggggcag gtggtgcggg ggtcagccgt
    19861 ccgaaccgga ggacagaccg gtcttgatga ggtccatcac cgacgtgtcg gccagggtcg
    19921 tggcatcgcc gacctgacga ccctcggcca cgtcgcgcag caggcggcgc atgatcttgc
    19981 cggagcgggt cttgggcaac tcgggaacga tgaggatgcg cttgggcttg gcgatcgggc
    20041 cgatctcctt ggccacgtgg ttgcgcaact cctcgacgat gccggtgttg tcgtcgtcgg
    20101 cgtggctgga gccggcgcgc aggatgacga aggcgacgac ggcctgaccg gtcatttcgt
    20161 cgttggcgcc gacgacggcg gcctcggcga ccttcgggtg gctgaccagg gccgactcga
    20221 tctcggtcgt cgacaggcgg tggcccgaga cgttcatgac gtcgtcgacc cggccgagga
    20281 gccagatgtt gccctcctcg tccttcttgg ccccgtcgcc ggcgaagtag tactgcggcc
    20341 agcgcgacca gtaggtctcg acgtagcggg ccgggtcacc ccagaggccg cgcagcatgg
    20401 caggccaggg cttggtgagc acgaggtaac cgccgtgccc gtccggcacc ggctcgccca
    20461 tgtcgtcgac gacctcggcg ccgatgccgg ggatgggccg ctgcgccgac ccgggcttga
    20521 ggttggtcac gcccggcagc gggctgatca tgatgccgcc ggtctcggtc tgccaccagg
    20581 tgtcgacgat cggggcgtga ccgccaccga tgacgttgcg gtaccacatc cacgcctcgg
    20641 ggttgatcgg ctcaccgacc gagccgagca tccggatcga ggacaggtcg aacttcgcgg
    20701 ggatgtccga gccccacttc atgaaggtcc ggatggccgt cggggcggtg tagaggatgc
    20761 tcaccttgta ctgctggacg atctcccaga accgaccctg gtgcggggtg tccggcgtgc
    20821 cttcgtagag cacctgggtg gccccgagcg ccagcggccc atagacgatg taggagtgac
    20881 cggtcaccca gccgatgtcg gccgtgcacc agtagacgtc gctctccggg tgcacgtcgt
    20941 gcacgacggc gttggtgtat gccgcctggg tgaggtagcc gcccgtggtg tggaagatgc
    21001 ccttcggctt ccccgtggtg cccgaggtgt agaggatgaa cagcgggtgc tcggcctcca
    21061 tgccctgcgc gacgtggacc gggttggccg cggccatcgc ctcgtgccac cacaggtcgc
    21121 ggccgtcgtt ccaggcaatg tcctggccgg tgcggcggac gacgaggacc ttctgcacgg
    21181 aggtgtcggc gtggtccagc gcggcgtcga cggccggctt gagtgcgctg ggggtcccgc
    21241 gacggtagcc gccgtcggag gtgatgacga ccttggcctg cgcgtcggcg atgcgggtgt
    21301 tgagcgcctc ggcggagaac ccaccgaaga cgaccgagtg cgggacaccg atccgggcgc
    21361 aggcgagcat cgcgacgacg gcctcgggga tcatcggcag gtagatggcg accgtgtcgc
    21421 ccttgcccag gccgaggtcg atcagcgcgt tggcggcctg ctgcacctca cggtgcaggt
    21481 cggcatacgt gacgtcgcgg gtgtccccgg gctcgccgac ccagtgcagc gcgaccttgt
    21541 ccccgcgccc ctcctcgacg tggcggtcca cgcagttgta tgccgcgttg agctcaccat
    21601 cggcgaacca cttcgcgaac ggcgcgttcg accagtcgag cacctcggtg aacggcttgc
    21661 tccacgtgac gtaacggcgg gcctgatcgc cccagaaccc ctcgtagtcg gcgtcagcgg
    21721 cggcatacag ctccgcggtg ccgttcgcct gagcggcgaa ggcggggtct ggcgggaagc
    21781 tgcggtcctc gtggagcagg tttgcgaggg tctcgttcga cacggaattt cctcctgcgg
    21841 cagggcgacg tgtgacctat gcaactgcca atgccgggtg gaggcaagtg aactgcacgc
    21901 gatgggggtc aggcgggcag tttgcgcggc gagcggttgc cgcggtttgt caggtgtgtg
    21961 acgtcacccg gtcgccggcg cgacgcatcg cctcgcgaac ccggtagacc tcggcctcgt
    22021 cggccccttc gtacaccagg gcctggacga ctcgacggat gtcgtcgtcg ggcaccgggt
    22081 cggcgacgga gtggcccgct cgcacccgca aggcacccac cacggcgctc gtctcggcct
    22141 cggtgagctc gcagcgcagc agcgccgggg cgaggaggtc cgctcgagcg actccgcgcg
    22201 ggcgcccttc aacgagccac ccacgtaacg actgcatctt caacccgccc acggtgccat
    22261 catccgccgg tcggcgaagg gccggggccc tggcggtcga acttctcccg acgacaacgc
    22321 gaacactcgg ggttgctcgc cctgggggcg actgcgcgcc cgcagttctg gccgtcgacg
    22381 ctgcccgtgc acgctgccca tcggcgctgt ccgtcggcgc tgtccgtcag agagatatgg
    22441 cggcccggca tccgtcgcgg aggtggattg ccgggtcgtc ctcgggatcc cgctcccgac
    22501 cccgcacgcg gcatactgcg tgggcttggt atgccgcgtg cgcaggtgag cgctccctgc
    22561 cggccccccc ggtcagtgcg ctgcgggcag cagacccatc cggtccttga cccgcgcgag
    22621 ggtggccgcc gagaccgcgc cagcccgttc ggccccgcgg gcaaggaccc ggtcgagctc
    22681 ggctgggtcg tcgagcaact ccgtcatccg gcgctggaac ggctctgcgg cggccacgac
    22741 cacctcggcg acctgggtct tgagcgcgcc atagccctgc ccggcatacc ccgcctcgag
    22801 ggccgggatc ggcaccccgg tgagggccga ctggatgacg agcaggttgg agaccccggg
    22861 cttgtgctcg gggtcgtagc ggatctccat ctcggcatcg gtgacggccg acttgatctt
    22921 cttcgtgatc cgtttggggt catccgacag aagcaccacg ccggcatcgg gagagttggt
    22981 gcccgacatc ttgctcgtcg ggtcggtcaa ctccatgatc ttggcggtct gcttgaggat
    23041 gtgcggctcg ggcacgacga gggtctggcc atagcgctgg ttgaagcgca gcgccaggtc
    23101 acgggtgatc tccaggtgtt ggcgctggtc ctccccgacg ggcaccttgt cggcgtcgta
    23161 gatgaggatg tcggcagcca tgagcatggg gtaggtgaac aggccgacgt tgccctgctg
    23221 ccccttggcc gtcttgtcct tgaactgcgt catccggctg gcctcaccca tcgcggtgag
    23281 acagctcatc acccacccga gctcggcgtg ctgcggcagg tggctctggc agaacactgc
    23341 tgagcgctcg gggtcgacgc cggcggcgat gaactgcgcg gccgtcacct gggtgcgccg
    23401 gcgcagcacg gccgggccgg tcggcacggt gagggcatgc aggtcggcga cgaagtagaa
    23461 cgcgtcgtag gtctcctgca gctcgaccca gttgaccagc gcgccgacgt agttgccgat
    23521 gtgcaggctg tcgctcgtcg gttgcatccc cgagagggat cgcggccggg accgcggggg
    23581 tatgccgctt ggctgggtgg tcatcgtctc tccttggtgc ggtccgtccc cggcacgaca
    23641 aacgccgccc caggggcggc gtttgggtgg ggttgcttcg tcgggcgccg ccgctatgcg
    23701 gtgcgccacc agaccttgag ggtcaacgaa gtcacaaggg aaggatagcg cccacctcgc
    23761 gcaggcggca taccggcggg gtcagcgcgc gaaggaggtt ggacgcggca tacctgaatg
    23821 cgggtgtgac caccgcaacg gcaggtatgc cgcgtccggt cttagggctg ccgcggagcc
    23881 ggtggcagcg agtcgccgtc gcgcaggggt gccagcggcc atccgccggc ggcgagccgg
    23941 gccgagacgc gggcgacgga ctcgtcgtcg gcgtcctcgt ggatccggtc ctcgatgagg
    24001 gctcggacgt cctcggcagt gaggggtggg ctgtctgccg cctcggcggc aagatcgccc
    24061 gcaagtgcct ggacctcggc agtggtgagc cgccggtgca ggatgcccag cagggcgatg
    24121 tagtccccgg acggcactcc ctccggataa cccccgcgaa gccagtcgag gatgcgggcc
    24181 gcgatcgagg gcgaacttgt catgccatca cctacttcga gacgagcgtg gggtagaagt
    24241 tctggaacga cagcgtcttg ccgaagccgg tggcgacgat gtaggtgatg cccagcgcga
    24301 tcccgatgac gacgagggcg aagcagagga tcgccaggac cttgccgatg gggtggccgg
    24361 cctcgtggtc gacctcggcg tcgcctccgg cgccgtaggc gagtgcgcga atgccgaggg
    24421 cgaagatcgc cggtaggccg gcgccgagga ggaccccggc gacgaggacc ttccacgcgc
    24481 cgtcgaaggc caatccgatg ttgttcatgg gtcggtcctt tcagatccgg gccggggagg
    24541 tggccaaggt ctcggagagt ttgctcgcgt cggtgtccca cgcgtcgttg acgttggtgt
    24601 ggtcgaccgg ggcgcgccgt gatcggaccc acatgtaggc ggcgagtccg acgaggaggg
    24661 cgaagaccac gaccgctccg aggagccctc cgaggaggtg gccgatgaac cacatggccg
    24721 cgccgacgag ggcggcagcc gggagggtga tggtccaggc gacgagcatc cgcgcggcaa
    24781 cgccccagcg gacctcggcg cggcgaccga ggccggtgcc gaggatggaa ccggtggcga
    24841 cgtgggtcgt cgagagggcg aagcccatct ggctcgaggc cagaatgacc gctgccgaag
    24901 aactctcggc ggccatgccc tgcggggagg agatctcgac caggcccttg ccgagcgtcc
    24961 ggatgatccg ccacccgccg aggtaggtcc ccagggcgat ggcgacggcg cacgtgacct
    25021 tgacccacaa cggcacggcg gtgaggtcgc tccactggcc ggcgccgatg agggcgaggg
    25081 tgatgacgcc catcgtcttc tgcgcgtcgt tcgtgccgtg ggcgagcgag acgagggagg
    25141 cggagccgat ctggccccag cggaacccgg cttcctggaa gcgttgggcg acgcctgcgg
    25201 tgatcttgta gatgagccac gtgccgatgg tcgacaccac caccgcgatg acgggggaga
    25261 ggagcgcggg catgatcacc ttgcccacga ccccgtcgag tttgctgccg tcgccgaccc
    25321 agttgacacc ggagagtccc agcccggcga tcgccgagcc gacgagcccg ccgaagaggg
    25381 cgtgggagga cgacgaaggc aggccgagca gccaggtcag gaggttccag acgatgccgc
    25441 cgcagaggcc ggccaagacg atgagcagca gcgcatagcc gccatcggcg agcagggccg
    25501 cgctcggcgc cccgctcttg tcctggatct tgacgactgc gttggtcacg gtgagcgcga
    25561 cctggacgga gaggaaagcg ccgacgaggt tgagcacggc gctgaggagg acggcggctt
    25621 tcgggcggag ggcgcccgtg gcgatcgagg tcgccatggc gtttccggtg tcgtggaaac
    25681 cgttggtgaa gtcgaaggcc agggctgtcg cgacgacgag gccgaggatg acggttgcgg
    25741 tggtcacgct tacatagtgc ggatgccttt gcccgaattg tcgggattgg gctctccgat
    25801 gtccaagtca tcggccactg ttcatgcccc gtatgcgggg cctttcagtc aacctcgttc
    25861 gaacaacaca gcgccccgaa ccttgtcggc ccggggcgct gcggctggtg agagaggtat
    25921 gccgcgcggc tcacgacagt gggagccgca cggcatacct cgtgctgtgg tgcggtggtg
    25981 ctgggtgaag ccggtcgtca gtggctgacg gccttctccg cgccggcgcc ggtgagggag
    26041 cggacctcca tctcggcgta cttcgcggcg ttgtgctcct tctcggtgac cgaacccagc
    26101 cagccgagga agaaggacag ggggaccgag atgatgcccg ggttgttgag cgggaaccac
    26161 gcgaagctcg ccgtggggaa catcgatgtc gccgaacccg acacgaccgg gctgaagatg
    26221 atgagcaaca gcgagctggc caggccgccg tacatcgaca tgagggcgcc gcgggtcgtg
    26281 aagttcttcc agaacagcga gtagaggatc gtcggcaggt tggcgctcgc ggcaaccgcg
    26341 aaggccaggg cgacgaggaa cgcgatgttc tggccgttgg cgaagatgcc accgacgatg
    26401 gcgagggcgc cgatgacgac cgccgtgcgg cgggccacct tgacctcgtc gtccgggctc
    26461 acggtgccac gcttgatgac ctgggcgtag atgtcgtggg cgaaggacgc cgatgccgtg
    26521 atggtgagtc cggccacgac cgcgaggatc gtcgcgaagg cgaccgcgga gatgacgccg
    26581 aggagcaact caccgccgag gtggtaggcc agcagcggag cggccgagtt gacgccaccg
    26641 ggcgcggcct tgatggcggc cgagccgacg agcgcctgcg cgccgtaacc gaggacgagg
    26701 gtgaacaggt agaacaggcc gatgagccag atggcccaga cgaccgaacg acgagcttcc
    26761 ttcgaggtcg gcacggtgta gaagcgcatg agcacgtgcg gcagaccggc ggtgccgagg
    26821 accagggcga tcgacagcga gatgaagtcg atcttgttct tgtactgcag gccggggttg
    26881 aggatggccg cgttgccggc ctgcttgatg gcgtcgtcga agagcgccga gatgttgaag
    26941 ccgtacttca cgccgaccca gatcgtcatg atgccggcgc cgaggatgag cagcgcggcc
    27001 ttgatgatct ggacccaggt ggtgcccttc atcccgccga tgaggacgta gacgatcatc
    27061 agggcgccga cgatggcgat gacgatgctc tgacccgtgc ggtcggtgat gccgagcagc
    27121 agggcgacga ggccaccggc gccggccatc tgggccagca ggtagaaggc cgacaccgcg
    27181 agggtgttga ccgcggcggc ggtgcggacc gggcgctgac gcaaccggaa cgagagcacg
    27241 tcggccatgg tgaaacggcc ggtgttacgc agcaactcgg caacgagcag gagcgcgacg
    27301 agccaggcga cgaggaaccc gatcgagtag aggaacccgt cgtagccgtt gatggcgatg
    27361 gcgccggcga tgccgaggaa ggacgctgcc gacaggtagt cgccggtgat ggcgatgccg
    27421 ttctggcggc ccgagaaggc gcggccaccg gcgtagtagt ccgcggcggt cgtgttctgc
    27481 ttggacaccc ggatgacgat gcccagggtg atgaggacga acaggccgaa gatggcgatg
    27541 ttcagggtcg tgttgccgac ctgggtcgcg gcgggcataa ccagggcgtt cactggaggt
    27601 tgcccctttc catgtggtcg gcgagcttgg cggcggcggg gtcaaggacg cggtcggccc
    27661 agcgggcgta gatcatcgtg atcgcgaacg tcgtcgcgaa ctgcgccagg ccgaggacca
    27721 ggccgacggt gatgttgccg aagagcttcg ttgccatgaa gtcgctggcg taggtcgcga
    27781 ggatgacgta gaggaagtac cacgcgagga agagaccggt catggggaac acgaacttgc
    27841 ggaaggtctt ccgcagctcg gcgaactcct gcgagttctg aacggcgatg tacctcgccg
    27901 cgcgttcctc gctgatgtcg ttatggctgc tcaaggtgta aacctccggt gggcaggcct
    27961 gcgaaagggc tccgcagtgt cgtgggtcac acaatgaggg cgagtcacga cggctgacta
    28021 gacgtcacgc gcccggtggg gtccgcgctc gcccagcggt cgggggcacc cgcccagcgg
    28081 ttggctactc agcccgccgg aggccgcacc cgggcggtca cttcttcggg ggcgtgcagg
    28141 cggaccatcg cgcgagcggc gttgacgggc accgaacgag gggtcgccag ggacacaagg
    28201 accgccgtgg tgaaggcgat cggcatcgac caggcggccg gttgggcgag cagcaccggc
    28261 agccacccac ccgttccacg gccgacgccg aagagggtcg ccacggtggc gcccatcgcc
    28321 agggttccgc cgacgatcag ccctgccgcc gcgccggtga ccgacaaccg gcgccaccac
    28381 acgccgagga tgagcagggg gccgagggtg gaggccgcga tcgcgaaggc catgcccacg
    28441 gtgtcagcga gggtcgcccc gggagcgagc cacgaggcgg catacgggat gaggatggcg
    28501 accagcgacg cgagccggaa gctgtgcgtg cgcgggaggt ccccgccgag gcgacggttc
    28561 atcgcgccgg agagcaggtc ctggtcgatg acccccgcaa ccgacacggt cagaccgctc
    28621 gcggtcgaca ggaacgccgc gaacgcgccc ccggcgagga tggcgctgag caggtcgccg
    28681 agggttccgg gggtgacggc ggagggcagc agcagcacga ccgagtcggc gcgggtgccg
    28741 gcgggcaggc tcggcaggta gacgcggccg agcatgccga gcaccggggg aaagaggtag
    28801 aacgcgccga gcagcgcgat gacgatgacc gtcgtgcgcc gcgcggcccg cccgtcgggg
    28861 ttggtgtaga agcgcaccag cacgtgtggc aaccccatgg tcccgaagca caaggcgagg
    28921 agcgtcgagt aggtcgcgta gagggggtgt tcccgcatgg ggtatgccgg tagcgcgccg
    28981 gcgaaggggc cgcccgggtc ggtgagcgag ggcagcggct gtccgccccg gaaccacaac
    29041 caggccagga cgaagcacgg gatggagatg gcggcgagct tgagccagta ctgcatcgcc
    29101 tggacgaggg tgatcgagcg catcccgccg gccgcgacgc tgacgagcac gacgacggcg
    29161 acgatgaggc tgccgacggc gggtgaggcg cccgtgacgg tctgcaacgc caggccggcc
    29221 ccctggaact gggggagcag atagagccag ccgatgccga cgacgaggac gctcgcgatc
    29281 cggcggacgc ggcgcgagga caaccgcgcc tcggcgaagt cggggatcgt gtaggcgccc
    29341 gatcgccgca acggcgccgc gacgaggacg aggaggatga ggtagcccaa cgtgtatccg
    29401 accggcagcc agagcatgtg ggtgccgagc gagtagacga tgccggccac cccgaggaag
    29461 cttgcggcag aaaggtattc gccgccgatg gccgaggcgt tccacccggc ggtgacgacc
    29521 cgtccggcga cgtagaagtc gctcgtcgtc cgggacatcc gtagcccgag cgcaccgacg
    29581 gcgacggtcg ccacgcacac gaggatgatc gcgaggagcc cccagccgga ggcgagcgtg
    29641 ttcatggccg ggtcgtcacc ggcccgccat cagatcggtg aagtcgcgct cgatccgttc
    29701 ggccatccgc acgtgcaggc gagcggcgag caggacgatc ggatagacgg ccacgccggc
    29761 gaccagccag atgacaggga ctggaccgag cgtggctgcc gcggcgccgg ggacgaggag
    29821 gaacagcgcg ggcagcccga cgaggatgcc gccgacggcg acgaggatcg ccagtgagag
    29881 gcgcagttgg gcccgcagca gggcagtcag gtagatctcg cccaagccgg tctgctcggt
    29941 gaactcgacc gcgtcggggc gcttctcctt gcgccgtcgg gcgtcccgac ggggtgagac
    30001 gacgcgcacc cgcttgggcg ccggccccac ctcggggagg ctccggtccc cacgtgggtc
    30061 aggcggccca gccgtggggg tcatggggtc accgtgcggc cgagcggatg agccggtcgc
    30121 gcaaggctcg ggtatgccgc ctgctcacct cgagcgcgat gtcgtccccg aggctcacct
    30181 tgcaccgccc actttgcacg gtgacggcgg tgatgtgggc tgtcgagacg agggtcgagc
    30241 ggtggatgcg cacgaacccg tgatcccccc agcgctcctc cagcgcgttg agcgagatcc
    30301 ggacgaggtg ggagccccgg tcggtgtgca gccgcgcgta gtcgccctgc gcctgggcgt
    30361 agaggatctg gctgcgctgg acgaacgtcg tcaccccgcc gagctcgacg gggatggttt
    30421 cgtcggccgg tgcgtcaggg accgggacga cggtgacccg tttctcggcc cgttgcgcga
    30481 cgacgcgccg cactgcctcg ccgaggcgat cggcgcgcac tggcttcatg acgtagtcgg
    30541 tcacctcgag ctcgaacgcc gtcaccgcat gctcgtcgta tgccgtgacg aagacgacct
    30601 ggggccggtc ggcgaagcgg gcgagcacct tcgccagcgc gagtccgtcg aggccgggca
    30661 tggagatgtc ggtgaagatg acgtcgaccg ggtggtcgtc cagggccttg agggcctcgg
    30721 tgcctgaccc ggcggtgtag atctcgccga tccgttcgtc ctgcccgagg agccaggcga
    30781 gctcggcgag ggtgggcgct tcgtcgtcga cgaggagcgc ccgcagcggc ggagacgacg
    30841 tcgtcgtggt catggagcca caggataggc cgctgagccg actgacgccg cagaacttct
    30901 gcttggggga tggcgcgagt ctccttttcg tgtcactatc agtaacatga aaaggagcta
    30961 ccgccagagc gagcgggccc ggcaggcgga ggagaccggg gtgcggatcc tgcaggagac
    31021 gcgccgcctc ttcgaagcgc aggggtatgc cggtctcacc ctggcgtcgg tggcggaggc
    31081 cgcgggggtc accacccaga cggtgatccg gcggttcggt gacaaggaag ggctcacggc
    31141 cgctgccggt cagcgggtgg cgcaggagat ctacgcccag cgtgacgaag caccggcggg
    31201 tgacctggcc gcgatcgtgc gcaacctcgt cgagcactac gaaacccgcg ggcccgtctc
    31261 gttgcggctg ctggccgacg agcacctcag cgaggcggtg gcctccttca cccgggccgg
    31321 gcgcgacttc caccacgggt ggtgcgagcg ggtcttcgcg ccgtggctgg ccgacctaga
    31381 cggctcgacg agccggcggc gggtcgcgca actcgtcgcc gtctgcgacg ttcgcacctg
    31441 ggagttgctc cggcaccgcg aagggctcac ccctgcccag acccgcacgg ctctgctcga
    31501 acttctccgc cccctcaccg aaggagcacg ccaatgacgg ccgtcctggc ctatacctcg
    31561 cctgcgatgg gccacctcta cccgatgatg ccgctgctgc ttgaacttcg ggatcgcggc
    31621 ctcgacgtcc atgtccgcac gctcgacacc ctcgtcgacc aggcccgagc cgccgggctg
    31681 agcgcagagc ggatcgacgc gcggctgcgt ccaatcgccg acaagcaccc cgattgggcg
    31741 gccaccaatg ccaaggcggc cctggagcgc agcgccacga tgttcgccga gcgggcggtt
    31801 ctcgacgggc cggacttccg ccgggccatc gaggaggtgg accccgatct gctcatcgtc
    31861 gacacgaacg cctggggcgc cctcgtcgtg gcagaggccc aacgccgtcc gtggctgcac
    31921 ttcagcccct atctcgtgcc cctgcgctcg acagggacgc ccccgttcgg ccccgggctg
    31981 ccacggtggg acaaccccgt gggccgggtg cgggacgcgg tcgtcgggcg cctggtcttc
    32041 ggggccgtcg agcgctcgat catcccgcgg atcaacgcgt tgcgaacccc tgccggcctg
    32101 gcccccgtcc gctccttcga cgacctcgtg ctggctgccc cactcgtagc gatctgcagc
    32161 gccacgcctt tcgactaccc acaccccgac tggccctcgc acgtcatcag catcggcgcg
    32221 atgacctggg agccgccgac cgccccgccg gcgtggctcg ccgaccttcc cgatccgctc
    32281 acccttgtga cgacctcctc ggaatatcgg gccgacgaag cgctcgcccg ggtcgccgtc
    32341 gccgccctgg cctccgagcc gggttcgctc gtcgtcacca tgcccgccgg tgtccctgac
    32401 gacctgcgcg acctgccccc gaatgtccgg gtcgagcagt tcgtgccgca tgcccccctg
    32461 gtggcccggg ccgcggtcgc ggtgacccac ggcgggatgg gcgcgaccca gaaggcgctg
    32521 gcgggcggca taccggtgtg cgttgtcccc tatggccgcg accaactgga gacggcagcg
    32581 cgggtcgtcc acgccgatgc tggcacccag ctggcgccga agaagctcac gccggcggcc
    32641 ctacgtgcgg ccgtgcgcgc tgccgccggc aagcgcgcgg gagcggctgc gtgcgcggac
    32701 ggtttccgag cggccggcgg ggcacgcgct gccgctgacg cccttctggc gcagttggcg
    32761 agcccctccc gccgctgact gctgcggccg ggggccgtcc gcgagtcgcc gcacgcttgt
    32821 atgaggctcg tgacgcttgt atgccgttac accctcatac aagtgccacc accctcatac
    32881 aagcggcgcg tcttccggcg cccgctcacc tcaggagccg gagtggactc caggcgcgaa
    32941 cttgggaacg cggaaggtga ccttggtgcc ggcgccgggg gcggtctcga cgaccaggcc
    33001 gtattcatcc ccgaaggcct gccgcagccg ggcgtcgacg ttgcccaggc cgacgctgtc
    33061 ccgcgagctc tcgccggcca gcacagcccg gatcgtgtcg gggtcgctgc cggcgccgtc
    33121 gtcctcgatc gtgatctcgg cgttcgcgcc gacgtcggac gcggtgatgg tgacgtggcc
    33181 gatcccggcc ttgtgctcga tgccgtgccg cacggcgttc tcgacgagcg gctggatcgc
    33241 caggaacggc accgacaccg cgagcacctc gggcgcgatg gtgagcgaca cgacgaggcg
    33301 gtcaccgaac cgggcgttct ccagcgcgag gtagcgctcg atgttctgca actcatcgcg
    33361 cagggtcgtg taggcaccgc cgcggcgcaa cgcgtagcgg gtgaagtcgg cgaactccag
    33421 caacagctcg cgggcccgat cagggtcggt ccggacgaag gacgcgatcg cgcccaggca
    33481 gttgtagatg aagtgcggcg aaatctgcgc gcgcaaggcc cgcagctcgg cctccatcac
    33541 ccgggtgcgt tggcgaccca gctcggcgag ctcgatctgg gtggagatcc acccagcgag
    33601 ctcctcggtg gcccggacca gcccggtcga ggtggttcgt ccgtatgccg cgagcgccgc
    33661 caccacgcgg tcgtcggcga cgatcggcgc gatgatcccc gagcggatcg agcagtcggg
    33721 gtccgagcag gtgatctggt cggaggtgag cacgagggtg cgccccgagc tgagcaccgg
    33781 ctcggcgtgg gcgcggacat cgccgcggtg gtgggacccg gcgccgtccc aggcgagggt
    33841 ggtggccgtg tcgacgatcg ccacggcggt ggagccgagc atcgagcgca ggtgccgggt
    33901 ggctcgggtg gctccctcgg gggtgagtcc gtcgcgaagt gatctcgtgg ccagcgacgc
    33961 cgtgtggagg gtttcgtaag tcgcccggtc gacagggtcg acgaaccggc gcctcccgac
    34021 cagccgcatg acgacggcgc cgagcaacag ggcagcgagc agcccgacgg tgacgagggt
    34081 gacctccccg ggagtgggca gctcgggcat ggcctcactc taggaggcgc gcggacggca
    34141 tacgctgcgg ttcgtgtccg atgctcgcgc tgctgtcctg accctgctgg atcttgaggg
    34201 ggtccgcgag gcggtcgacg ccgcgcgcga cgcctgcacc caactgcgct ggcacgaagc
    34261 gctgcgccga cggatccccg aagcgtcggc cgagtcgcgg gttcgtggct cccgggcgag
    34321 cgcgttcctc gacggggccg agctgcccgt cgacctggtc cgtgacctgg tgcggggcgc
    34381 gcgtccgtgg cccgagcacc tcgacccggt cgaggaggtg ctgcgcgggg cgatccaggc
    34441 gaccgccgag accgagcaca tcaggggtct gctcgtgcgc tcaccatcgc aggcgctggc
    34501 tcgactgcat gtcgccgcgg cctcggggct gctgccggtc gagcaggtgg ggcgcccgcg
    34561 cctgggcgag gagacctccc gcgagttcgt cgacctcggt atgccgcccg cccccgccga
    34621 actcccggct cgcctggccg gggtggccga gttgatcggg ctggccggcg aggtccccgt
    34681 cgtcctggcg gcagcgctcg tgcacgccga gttggccgtg gtgcgaccgt tcgtccgggg
    34741 caacggtctg gtggcgcggg ctgccgaacg ggccctcgtg gcggccgccg ggctcgaccc
    34801 caccggagtg gccgtgcctg aggcggggtg gctcgcgggt ggtgggacgg cctacctcgg
    34861 ggcgctcacg gcatacgcct ctgggactcc gcaaggcgtg gccctgtggt tgcgccaggc
    34921 cgcgcaggcg atgacccaag cggcggccga gggcgcgcgg gtcgccgacg cggttcgggt
    34981 cggtcggctg acctgagcgt gcggggccgc ttgaggactg tgtcgaggcc gacacgccaa
    35041 gcgtgtgttg cgaatggggt accgtttgcc tttcgggctc ggaagccgga gaatagtcat
    35101 gctccccctc ggggttgagc aggtgcgcac tggcccctcc cccccggtgc cagcgcgcca
    35161 gacggccccc gttgtccccc cagcgggggc cgtcgcacgt cccccttccg gccccggcca
    35221 gtggtgtggt gccctctttc tgccgtcagt gggctgaggt ggagtccgcg gcgtcgtgct
    35281 gtcggtgggc caaggcgtgg gcgcggcggt gggtgcagga tcaccatcgg cgggtcgcgc
    35341 gcttgctgag ttgtccacat ccccttggtc atccgtccca ccatccacag ggccgccggt
    35401 ggtgggtgcg ggccgggacg ggcgcagacg aggctggcag gcatggacgg atctgctggc
    35461 aggagcgcga ggggtgtcgt cctcgcggtc gtggg
//
LOCUS       DAOMZY010000002        34016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_955_length_34016_cov_5.891110, whole genome shotgun sequence.
ACCESSION   DAOMZY010000002 DAOMZY010000000
VERSION     DAOMZY010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 34016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34016
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_955_length_34016_cov_5.891110"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      87..230
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            317..1120
                     /locus_tag="PLK64_00190"
     CDS             317..1120
                     /locus_tag="PLK64_00190"
                     /inference="COORDINATES: protein motif:HMM:NF040672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO2322 family protein"
                     /protein_id="HPV78186.1"
                     /translation="MLSVTPSIAARRSASRRAFAVLAAVMVAFATLVSTAPQASAANY
                     LYWSYWQQTAGKWAFSSVGADKATPSDGAVEGWRWAIDDGSGSRPPRVLPTFEQLCAS
                     TPAEAGKKRVGLVVDFGRDVDGDGKVTPPSLVATCALVPTAATGADLLAKAGGVRTDK
                     GLICAIGGYPATGCAAQLETLTEAQKAPDTALALPTAAPTTAPTATTPVATTTTAATP
                     PATATAAVVATSTTAAAGGGTSPLLWVLLFVVAAGIAYAVARRRRPSGV"
     gene            1136..2269
                     /locus_tag="PLK64_00195"
     CDS             1136..2269
                     /locus_tag="PLK64_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HPV78187.1"
                     /translation="MARPTPAATPRMLHPVAWWVWGVGLATAAAHTTNPWLLLLVIAV
                     AGWVVLERREVGALDSFRPFLIIGLFAIGLRVALTILLGNGVPGQVVLVELPQVRLPD
                     WFAGLRLGGPVTLESVVAAGIDGLRLATTLACLGAANALASPRRLLRYAPATLYDIGT
                     AIVVALTFAPALVDLAGRVRSARRLRGHSGTGVRELARLGVPVLEGALDRSLDLAASM
                     ESRGYGRTAVRGRRERIVGSALALSGACGSVAGLFGVMSASSGGWFGLPMLVSGLALL
                     ATSLVLGSRRDARTAYRRDPWRAPEWATCALGLLPAVLLVHAEADAWPGITISQVPLD
                     WPPLPALPTLAILAAVLVAWVTPVPPRLARARATTLPARLETA"
     gene            2266..3888
                     /locus_tag="PLK64_00200"
     CDS             2266..3888
                     /locus_tag="PLK64_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPV78188.1"
                     /translation="MIELRGVGVTYAGASRPSLRDVDLRIEEGELVLIVGPTGSGKST
                     LLRTLNGLVPHFSGGTLTGSVVVGGRDTRAHRPRDLADLVGFVVQDPTSAFVTDTVED
                     EIAYGMETLGVEPTAMRRRLEETLDIMGLAELRDRSLRELSGGQQQRVSIAAVLAAGP
                     RVLVLDEPTSALDPVAAEEVLSALHRLVHDLGYTVVLAEHRLERVVQHADRIVLVRDG
                     VVSAPLDPAEAMALSPVHPPIVELGRRMGWSPLPLTIRDARRRAGSLRARLAALDGTP
                     AASAPVKDAASRSLVSVNRLVVRRGTVEALSKVTLGVSAGSVTAVMGRNGAGKSTLLA
                     AMAGQLAATSGEVSVAGSSPRRLAPHDRVRVVGLVPQDSNLLLYADSVAAECRAADRD
                     FAAADGTTRALLARIAGEVDGDQHPRDLSEGQRLALALAIILAGRPQVVLLDEPTRGL
                     DYPAKRRLVGILRDLAAEGHAVIVATHDVELVAEVAQRVIVLAEGEVVVDGPIHEVLT
                     GSPAFAPQVAKILHPLPLLTVDDVSAALVPAS"
     gene            3885..4664
                     /locus_tag="PLK64_00205"
     CDS             3885..4664
                     /locus_tag="PLK64_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPV78189.1"
                     /translation="MRFRPAAAAALALTTVVGLAAYLWPFFAAPGFSTSYGQDAPWLF
                     AALLGLMAFVVLAEVTANGLDAKTVAVLGVLAAAGGALRVLSAGTAGLEPIFVIFVLG
                     GRVLGRSMGFLMGSLAMLAGAFLTAGVGPWLPFQMLGAGWVALGAAMLPRATGRVELA
                     MLAGYGAFAGLAYGVLLNLWFWPFLAPASAPTGAGFVPGDSAAANFAHYAVFYVATSL
                     GWDVPRAVLTAVLILVAGRSILATLRRAVRRAAFGEVVRFD"
     gene            complement(4661..5761)
                     /gene="gcvT"
                     /locus_tag="PLK64_00210"
     CDS             complement(4661..5761)
                     /gene="gcvT"
                     /locus_tag="PLK64_00210"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HPV78190.1"
                     /translation="MADLAHSPLHDRHVALGAKLADFGGWEMPIEYPGGGVVAEHTAV
                     RERVGVFDVSHLGKATVSGPGAADFVNACFTNDLRRVGPGQAQYTLCCDATGGVVDDL
                     IQYLRADDDVFLIPNAANTAEVCRRLQAAAPEGIEVVDRHRDYAVIAVQGPRSDEVLD
                     ALGLPTGHDYMQFREADWQGRPVIVCRTGYTGERGYELVPRWDDAADLWDAIIEAMVP
                     WGGMPCGLGARDTLRTEMGYPLHGQDLSLDITPVMARSGWAVGWAKPAFWGREALLAE
                     KAAPTSRISRGLLVTGRGIPRAHCPVTLDGEVIGEVTSGTFSPTRKQGIALALLDRSV
                     PLDAEVLIDVRGRGVEATVMNPPFVQVQTRED"
     gene            complement(5834..6034)
                     /locus_tag="PLK64_00215"
     CDS             complement(5834..6034)
                     /locus_tag="PLK64_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003406063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5302 domain-containing protein"
                     /protein_id="HPV78191.1"
                     /translation="MSKNAKSGKAPKAAGPSDEMKAKFREALERKQAHAGRESAGEGE
                     GKVHDAHGPAAAQRMFRRKSGG"
     gene            complement(6052..6429)
                     /locus_tag="PLK64_00220"
     CDS             complement(6052..6429)
                     /locus_tag="PLK64_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78192.1"
                     /translation="MGLFDRWRRSSLPDLGRSDGPAMRVDLATVRGHFAQFVQTRRGV
                     EAFLEPATNVSTQSVVLVATDGEWTRRAVGSRAAAYEMAAALGIPIYDVLLTGYPSRM
                     REWSSRQRRDKRGDTRPGSRSDD"
     gene            6453..7586
                     /locus_tag="PLK64_00225"
     CDS             6453..7586
                     /locus_tag="PLK64_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78193.1"
                     /translation="MSTRGTSTGRWAGVGVAGVIILSLASIGAHALLIPGFIQAADAS
                     SRSTGALSTPTTVPSGQGGTGSLSSTSSPAATDLAPTAVPAMPAYDRACLRRDNPATG
                     AKIPANDPFGDDIADLGDKAELQRLYAVQSGALVPLDGLGAPRPCDEQLWSLVKATAP
                     TLLGHIDELLVFDADPDPATGEFIIEGESAPKETAPDTFDDDHWRVSFAPNGLDRGEL
                     AWLVAHELAHVASLNKDQMLSGVGADVCATWYTGTGCLLADSFLRRYLSNTWDDALWD
                     DWDKADGKSTEKARRAAYQDFYDNHSDSFITEYAAWHPLEDFAESFAMWCTYDEDEPD
                     RANLPTAERTDSGNKVAWFDAARRDLLPAFGPGCTMLRQFAVS"
     gene            7680..9059
                     /gene="lpdA"
                     /locus_tag="PLK64_00230"
     CDS             7680..9059
                     /gene="lpdA"
                     /locus_tag="PLK64_00230"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPV78194.1"
                     /translation="MTDEAPDIYDVLVLGGGSGGYACALRSAQLGLRVALIERDKLGG
                     TCLHRGCIPTKALLHAAEVADTAREGARFGVNSSVESIDMSAVLRYQQGVVSRLHKGL
                     QGLISAATIDYVDGFGEVTSPTTVRVGDRTLTGRRLVLATGSRARSLPGLEIGGRVLT
                     SDQALGLTQVPTSVIVLGGGVIGVEFASVFRSFGASVTIIEALPRLVPAEEESISKAL
                     ERAFRKRGIVSRTNARFASVAQDDASVTVSLEDGSTLSADLLLVAVGREPVSDGMGFQ
                     EAGVHTDRGFVPTDERLRTNIPGVYAVGDLVPGLQLAHRGFAHGVFVAEDIASLNPAP
                     VLDRNIPKVTYCDPEIASVGLTEAAARAEHGEVRAYEYNLGGNGKSQILGTSGFVSVV
                     RVVDGPVVGIHMIGARMGEQIGEAQLVVGWDAMPEDVAPLVHAHPTQNEALGEAHLAL
                     AGKPLHAHA"
     gene            9129..10949
                     /locus_tag="PLK64_00235"
     CDS             9129..10949
                     /locus_tag="PLK64_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HPV78195.1"
                     /translation="MSNRVTMPALGESVTEGTVTRWLKKVGDPIAIDEPLVEVSTDKV
                     DTEIPSPFAGIVEAILVAEDEVAAVGADLAVIGDGSGAAAAPAAPAVEAAPAPAPAVA
                     PAPAPEPAAVVVAEPAPAPAPAAAEASGDGHEVTMPALGESVTEGTVTRWLKKEGDTV
                     AVDEPLLEVSTDKVDTEIPSPFAGVLSRILVGEDEVAAVGAPLAIIGGSASAPAPAAA
                     PAAAPAPVAAPAPVAAPAPQTVAPAAPAPAAAPAPAASGDEGDSDGEGASYVTPLVRK
                     LAALHKVDLASLVGTGVGGRIRKQDVLDAAEAARNAPAAPAPVPAPAAPAPAAAAPAP
                     APAAPAPVAAAPAAAAPAASAAPASARPSLQPSSKRGTTEKMSRLRKVIASRMVESLQ
                     VSAQLTTVVEVDLTRVARLRARSKAAFQAREGTSLSYLPFLALAAVEALKVHPMVNAS
                     INGDEVIYHGQEHLSIAVDTDRGLIVPVVKNAGDLNIAGLARGIADVANRTRTNKITP
                     DDLTGGTFTITNTGSRGALFDTPIINQPQVAILGTGALVKRPVVVSDAEGGETIAVRS
                     MMYLALSYDHRIVDGADAARFLETMKHRLEEGNFEPDLGL"
     gene            10960..11865
                     /locus_tag="PLK64_00240"
     CDS             10960..11865
                     /locus_tag="PLK64_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HPV78196.1"
                     /translation="MSGRRVAVTGASGFIGGALTAYLAGRGDEVVRLVRHQPTQPSER
                     RWDPVRGGLDPRAIDDVDAIVHLAAAGVGDHRWTPAFKELVLRSRVDGTAAVATAIAQ
                     TGRPIRFVCGSAIGYYGDRRDEILTELSGPGTGFLAEVARAWEGSAEPAIAAGAPVAF
                     ARHGLVMGPGGGAFARLLRLARWGLFGPLGSGRQYWSWITLADSVRALSHLIDRPDLT
                     GPVNIVGPDPRPQRVVASAIGHALHRPAMLPAPSVALRVALGEFADDILGSQRVMPFA
                     LTGSGFTFEHSMLRPAAAWLVRESS"
     gene            complement(11911..13446)
                     /locus_tag="PLK64_00245"
     CDS             complement(11911..13446)
                     /locus_tag="PLK64_00245"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPV78197.1"
                     /translation="MSAAKTSPPSVPGLVLGQVIGRGATSTVWSAVRVDDGRAVAVKI
                     TSPDRLHVGQLMELAARETAILATVEHDHLVRLHEAHALPDGSVAVVLDLAPGGSLAD
                     LVAARGRLDGGEVATICTPLAGALAALHAAGVVHGDLSPGNVLFTADGKPLLSDFGAA
                     RLVGEGHPPLVAGTSGFVAPEVVAGDVPGEAADVFGLGALAWFALTGRSWSVRSWATP
                     LGGADTSVPGPVTVDEAVAVLGAGFGPVVAAMLSQDPSGRPEAQEAAVLAYQAASPVA
                     VRLVGSAAGADPDRVLTQRLRGAAGRVTPGVGAGPGRSQAVRRGQAARRGRAGGRVPG
                     PRVGRKARLGLGAALAIVLAAVVVSVFLRLSPAAAAEGSAGEVTRVLGSAAAPAGEAR
                     AVLATLVGRRAQALTARDLRALAAADGQASTLLAADEAIVAELVQAEQRYVGLTFTVT
                     VAEWVSVDAGSARIRAVVERSGYRVQGPGDREQSIGADSGRSLLYSLVRVGEEWRIST
                     VTG"
     gene            complement(13557..14594)
                     /locus_tag="PLK64_00250"
     CDS             complement(13557..14594)
                     /locus_tag="PLK64_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPV78198.1"
                     /translation="MTRVGLVGYGSSGAGFHAPLLREAGLDLVAVVTGNPERADQARA
                     ENPGVVVVPDLAALVAVPDLDVVVVASPSGRHVEHAEALLEAGIAVVVDKPIATDAHQ
                     AQGLVDLAAGRGVPLTVFQNRRYDPEFATIRDLLHRGVLGELRRTEMRWDRWRPVPKD
                     RWRERATAAEGGGLLLDLHTHLLDQVVLMHGPVATVYAELAAWTVTAEDDTFVALRHT
                     DGYSTHLSASSVAGAPGPRARITGSAGTFLLGSAGGEPTAFHDADGGPGQHGWLVRGD
                     EREAVLARSSDPADFYREVAAALASPDPQESMPVDPRDAVHVLAVIDAARISAADGRV
                     VDVITPGEDPH"
     gene            14666..15364
                     /gene="lipB"
                     /locus_tag="PLK64_00255"
     CDS             14666..15364
                     /gene="lipB"
                     /locus_tag="PLK64_00255"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HPV78199.1"
                     /translation="MPSRRVKVGLMRVVHLGFDPDFVDYEWAWAHQREVHAKVVSGEE
                     PDTVLLLEHQPVYTAGKRTEAHERPNDGTPVVDVDRGGKITWHGPGQVTGYPIVRLPA
                     PIDVVAYVRLLEEMMIRVCADFGVSAGRVDGQSGTWILADPADPRRRPNRKIGAIGIR
                     VSRQVAMHGFAFNANPSLAWADVIIPCGIPDAGVTSLSAEVGRDVPVREVLPHIEKHL
                     ADVLGTLAPNRRAI"
     gene            15440..16477
                     /gene="lipA"
                     /locus_tag="PLK64_00260"
     CDS             15440..16477
                     /gene="lipA"
                     /locus_tag="PLK64_00260"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HPV78200.1"
                     /translation="MTIAPEGRRMLRVEARNAQTPIERKPEWIRTTAKMGPAFTELHS
                     MVKGEGLHTVCQEAACPNIFECWEDREATFLIGGDTCTRRCDFCDIATGRPQPLDPDE
                     PRRVAESVRRMGLRYSTVTGVARDDQPDGAAGLYAATIRAIHELNPNTGVEILPPDFG
                     AKPELVGQVFDARPEVFAHNLETVPRIFKRIRPAFTYDTSLRVLSMAREAGLVTKSNL
                     ILGMGEEAQEIEAAIVDLHDAGCDILTITQYLRPSAKHHPIDRWVKPEEFLHWSSVAT
                     EHGFKGVMAGPLVRSSYRAGRLWAQAMARWGRPVPEHLAHLAEAAGAPARQEAVAVVA
                     AFAERSGAPVS"
     gene            16483..17211
                     /locus_tag="PLK64_00265"
     CDS             16483..17211
                     /locus_tag="PLK64_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4191 domain-containing protein"
                     /protein_id="HPV78201.1"
                     /translation="MAKDPSSTAAPKKPGRIAQLRTVLQQSKALDPKIVWWMAGAFVL
                     TLAVIVGIGWLVNWPVYAAILGLPLAALAATIVMSRRAEGAAYAKLEGQPGAAGAALT
                     SLRGGWFTSSEPVAVEGARSGSMADAALVYRAVGRPGVVLVAEGPEARAQRLLLAEKK
                     RTERVAPGVPVTVYRVGSGKEEGVVEIRKLTSKVQRMRATLSKDDVLKVNQRLRALGG
                     VKLPVPQGIDPARARPDRKGMRGR"
     gene            complement(17490..18185)
                     /locus_tag="PLK64_00270"
     CDS             complement(17490..18185)
                     /locus_tag="PLK64_00270"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPV78202.1"
                     /translation="MSGSPADLADVLRSAAKGEFPAADGGWERASLWLRGVEAVVAMT
                     GHAYLAVGQDLADVELDAYGVDGYGGAHHPRVAELIRGSGWADALDVLLVRPPDAPRP
                     TATGPDLVSRDDLAGHPRVAVAQHVRSGIRVLGMPSRRVRSVVTVASGLGGLPEIGLQ
                     TEGPTSGRELLDAALRWVDDELGHSDPLVAAVAPGNARSLRLFLHVGFVPVGSVQLFR
                     PERHPWQDDTAAE"
     gene            complement(18182..18646)
                     /locus_tag="PLK64_00275"
     CDS             complement(18182..18646)
                     /locus_tag="PLK64_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015936803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPV78203.1"
                     /translation="MVDRRDVASWLEGPRARTQAPGEYPGQDLGMPPSGPGSIARFGR
                     RLVAICIDWALCQLIAVFVFRAELGQGGPGSFAPLAVFAVENLLLLPTLGATVGQRLL
                     GIVIMTVDRRRISVVQATLRTLLLCLAVPALIWDRDSRGLHDKAAATVLVRR"
     gene            18832..20256
                     /gene="glnA"
                     /locus_tag="PLK64_00280"
     CDS             18832..20256
                     /gene="glnA"
                     /locus_tag="PLK64_00280"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HPV78204.1"
                     /translation="MFTSADEVLAYIKAENVKFVDIRFCDLPGVMQHFNVPAHTLDED
                     FFTVGQAFDGSSIRGFQAIHESDMKLLPDVATAYLDPFRVEKTLVINFSIVDPFTDEP
                     YSRDPRNIAAKAEAYLKSTGIADTAFFGAEAEFFVFDDVRFKTSANESYYYVDSKAAV
                     WNTGKVEEGGNLGYKPRMKGGYFPVSPVDHFADLRDEMCLRLAEVGMEVERSHHEVAS
                     GGQQEINYRFNTLLHSGDDMMKFKYVIKNTAWANGKTVTFMPKPIFGDNGSGMHTHQS
                     LWKDGKPLFYDESGYGGLSDLARWYIGGLLKHAPALLAFTNPTVNSYHRLVPGYEAPV
                     NLVYSARNRSACIRIPIAGASPKAKRIEFRIPDPSCNPYLAFAAQLMAGLDGIKNRIE
                     PPEPVDKDLYELPPEEHAEIKQVPGSLPEVLSALEVDHDFLLEGGVFTKDLIETWIEW
                     KRKNEVDPIRFRPHPHEFEMYFDI"
     gene            complement(20596..20883)
                     /locus_tag="PLK64_00285"
     CDS             complement(20596..20883)
                     /locus_tag="PLK64_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78205.1"
                     /translation="MDGSVVRHAPGCALDPVNWFRHAIAVAARLALQDVDRAQHDQVS
                     AAALLDTLGLIAELPEWGEVTLREVARQATIMTDDVFVGMCTCGAARDAGL"
     gene            21007..22557
                     /locus_tag="PLK64_00290"
     CDS             21007..22557
                     /locus_tag="PLK64_00290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPV78206.1"
                     /translation="MSPTPSGLFHDVPIEDLFFSTTDAKGVIDEANEVFARNSRYERS
                     ELLGAPHNIIRHPDMPGAVFKATWDLLGAGHPVCAYVKNLARDGSTYWAFATIVPIAG
                     RFLSVRATPCDRTARDLFESLYATVREAEIAARDAGASAREAADVGSEVLGKALAENG
                     FGSYTELQLDLVPVEVTAREQVCPPLPQLPDGSKTARKILAQVTAARSSLGAFAADIA
                     DSLTLTDTLSRDLRRLGAALAGLDRAVASAAQTLTAATPAMATGSTMVTYASDPVADS
                     ADMTVGAVGAVGTVEADVAALVPIIQSRLGSLRDDVERAGDELASVVRARKHLALGSA
                     IARMQAEAIGRYVVAVEAGAEDPRVSERALSSLSTALLAILDADLLTDREATTAYVDR
                     VARLATEVAGARETTVAWRSLLESTMPRAATNDGSVTDDGSVTDDGSEAGNGSVTGDR
                     PEDGSDDHSDAAHESRLAFDVALSAANDQMGRVADAVGAVAATNAALDRDELAAVLGK
                     IVELAAKI"
     gene            complement(22631..23527)
                     /locus_tag="PLK64_00295"
     CDS             complement(22631..23527)
                     /locus_tag="PLK64_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPV78207.1"
                     /translation="MSGDRRVHLDRLAVSLVIGCTVVWGLGQVASKVALDEVPPLTQA
                     GLRSIGAALLVLAWAKLRGIPLWERDRTLGPGLVAGALFAAEFAAVYSALQFTTAGRM
                     TVFLYTAPFVVAIGMPFIARAERLTGLALAGLIAAFAGVGFAFADSFTSAGAGSQQWL
                     GDLLAGLAALGWGATTLVIRATALAPIHPAKTLLYQLGVSGVGLTAIGWFVGERAQWP
                     LSMTVAAALVFQVVVVGSTSFVVWFWLIRTYSATKLSAFTLLTPVVALVGGAVLLREP
                     VSPRIVVALVGVCLGLVAVNRR"
     gene            complement(23615..24001)
                     /gene="trxA"
                     /locus_tag="PLK64_00300"
     CDS             complement(23615..24001)
                     /gene="trxA"
                     /locus_tag="PLK64_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPV78208.1"
                     /translation="MSTIDLTAATFEQTVTENDLVLVDFWAAWCGPCRMFAPVFQKAS
                     DENPDVVFAKVDTEAEQALAAMAGITSIPTLMAFREGVLVFAQPGALPAPALAQVIEG
                     VRGLDMAEVHGQVAAQRAQSGAVADA"
     gene            complement(24039..24491)
                     /locus_tag="PLK64_00305"
     CDS             complement(24039..24491)
                     /locus_tag="PLK64_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPV78209.1"
                     /translation="MTFRRLAVIVATVAVAAITVGACSGSAGSGSAPLASAAAATAAP
                     ANGAEVDAAGFAAALKRPGTVVLDVRTPAEFAAGHLAGAVNVDVNAADFRTIVGGLAK
                     DVPYAVYCRSGNRSATALAIMKGLGFTNTFHLGGGIQAWTAAGGAVTQ"
     gene            complement(24668..27709)
                     /locus_tag="PLK64_00310"
     CDS             complement(24668..27709)
                     /locus_tag="PLK64_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPV78210.1"
                     /translation="MNRTSVGALVAIPILVGATLMGSGSAGATPLSAGAAPNAAAIGA
                     SAPAASGAAYSDGTYIVQMRDLPVASYDGSLAGYTATKAKAGKKFDSTTAAATKYASY
                     LTGSHDSALNKVGGAAKLYDYLFSFNGFAAKLTGAQAGALAKAPGVVAVTPQELRKGD
                     TTSTPSFLGLNDAATGGLWTPAGGNLKGEGVIIGIIDSGIWPESLSFTDRVDANGNPA
                     AQGFLAYQQIPGWHGKCTPGEGFNASMCNQKLIGAQYFNAGWGGNGGVAEEEFASPRD
                     FGGHGTHTASTAGGNAKVPTTQDAARIGAVSGLAPRARIAAYKALWETPSGGSGYNSD
                     LVAAIDRAVADGVDVINYSVSGSQTNFADPVEIAFLYAADAGIFVAASAGNSGPEEST
                     VAHPAPWTTTVAAATHNRNGVGSVTLGNGATYEGASFATPVGPAPLIDASAAGLTGAD
                     PENLAWCVGATDGEVTLDPAKVAGKIVVCRRGETARANKSLAVKEAGGVGMILFNSPD
                     NSLNADFHFVPSVHVNQAAGEAINAYAATAGATATINQSTVIYDAPAPFTAVFSSRGP
                     LIAAAGDLLKPDISAPGQDVLAAVAPPGNAGRDFDLYSGTSMSAPHMAGLAALLKQAK
                     PSWSPMAIKSAFMTTAGDVLDGPNTSATVIFNQGAGHVTPNKATDPGLVFDSGWNQWL
                     AFLCGSTSAVGPSTCAKLAAGGFLTDPSDFNSASIAIGSLAGTQTVSRTVTNVGTERA
                     TYSLTTSGLAGITVTADPQTFTVAPGRSQTVKLTFMRSTAELNTYAGGQLTWSDGTHN
                     VRIPAVIRPVALAAPTQVNGSGAAITYQVGFGYTGPFTATPRGLIPAVKTPGHVLDDP
                     NDSFSPTGLGVTAHEVVIPAGTTYARFSLFDDFVDGAVDDLDLYVYLGSKRVGGSGSG
                     TSAEEVSLVNPAPGTYTVYVHGWGTDGPDANYTLFQWLLGSTDAGNMTVTAPATATIG
                     QTGQIVLSFNGLLAATKYLGSVAYTGEASATNPTIVRVDMP"
     gene            complement(27901..29598)
                     /locus_tag="PLK64_00315"
     CDS             complement(27901..29598)
                     /locus_tag="PLK64_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPV78211.1"
                     /translation="MQLNLTGLSMPRSSHAVTTLLNAVLAVGSGLDLSEVLTRIVRSA
                     CDLVGARYGALGVLRPDGEGLMEFVTSGLTPEEAGQVASQPFGHGVLGTLIRDPRPLR
                     LSAVGEHPDAVGFPPGHPVMTTFLGAPIHVRGEVFGNIYLADKVGGLEFTGEDEELIV
                     ALAAAAGMAIDNARMFHMARSGRQWSDALREMTQALLYGRDQRVALSMLVQRAQLAAE
                     AELAAVALAGEDGQLIIEASTGTDPRSRLIVGTPLAEPHWVTILDSGEPLLLLSREGE
                     AVADSLAAQLREASGLPPHGQTAIVPMSVGTGSLGLLVVAWGEGESHTAYDAAEPLKQ
                     YADLAAVTLTAASAQRDRARMVVLEDRERIARDMHDVVIQRLFATGLGLQSASRVAAH
                     PYVQKRLSEAVDELDLAIKDIRRMIFALHQPESQPLSEELHDLVVSFAEPLGFTPRLT
                     VVGPLDDPETVDEWLRLDLLAVIREALSNVARHAQAGEVDVAVVVQADLVQVTVEDDG
                     VGMIETGRRSGLANLSERAAGRRGSLVVLSPMAVLGGTRVIWEVPASVGAGPTGDPL"
     gene            complement(29622..30047)
                     /locus_tag="PLK64_00320"
     CDS             complement(29622..30047)
                     /locus_tag="PLK64_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPV78212.1"
                     /translation="MSTTRAQQGVPYTPGRSGDVVAGLLNDGRAESVAKAAVTEAVRR
                     GARLRFVQIVEEGLSPDERAEADQATFRAAVHALRGLRGIPCRFEVVTGDPTPTLLEL
                     TRGAAALIVGTDEGTARTIARRCVAHAECPVVTVPISAA"
     gene            complement(30165..30815)
                     /locus_tag="PLK64_00325"
     CDS             complement(30165..30815)
                     /locus_tag="PLK64_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPV78213.1"
                     /translation="MTITVFLLDDHEIVRRGLRELLELSGDIEVVGESASALEAERRI
                     PALRPKVCVLDARLPDGSGIDVCRAVRAVDPTLRALILTSYDDDQALKAAVLAGASGY
                     VLKDIKGNDLVDAIRRVAAGEHLIRPEVMARVKSSLGESSVDPRLRSLTPQERRILEH
                     IAEGLTNRQIAEDMFLAEKTVKNYVTSILDKLGMDSRTQAAVYAATHPTRSRPRRP"
     gene            complement(30855..33914)
                     /locus_tag="PLK64_00330"
     CDS             complement(30855..33914)
                     /locus_tag="PLK64_00330"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HPV78214.1"
                     /translation="MVDRSSSAVALMARLGFAEPERSAGLLRDAVLLRLFAPGGDPGA
                     GATLDTDALIESTRAVRTSVAEGLASAADPDLAALGLVRLVEAVLGMPPAHHSDPRAL
                     AEVLSTDSVRRRRLLLTLGGSRALVDHLAAHPEHWVAATDATPPTGPGLRTALTSAVT
                     PQARGAATAYDALRVAYRRELVGIAALDLSDPDPVSHLPAAAEALAQLAEAALEAALD
                     IAREEFGPGHEDCRLAIIGMGKTGGGELNYVSDVDVIFVVEPADGVDEERAIKVGTAL
                     ATATMKACAAQTGEGTLWPVDAALRPEGKAGPLVRTVASHKAYYERWAKTWEFQALLK
                     AWVSAGDREVGIAYKDALRSMIWGAAGREHFVEDVQAMRRRVEQHVPAAEAARQLKLG
                     PGGLRDVEFSVQLLQLVHGRHDESLRSATTLQALHALASGGYVGREDAATLDAAYRLL
                     RTLEHRIQLYRLRRTHLMPTAPDELRVLGRALGLTSKPAEAVVAMWQSQAREVRRIHE
                     RLFYRPLLAAAARLSSDDTSLSPQQARERLAALGFRDPAGAMRHLETLTAGVSRRAAI
                     QRTLLPVMLSWFADEADPDAGLLAFRRVSDDLGSIHWYLKMLRDEGSAAERLAHVLGR
                     SRYAADLLIRAPESVSILGDPDGLRPRSQESLAITLRSAAARKDNPDKAMAAVGALRR
                     HELLRIVFGDLTGQFAADQVGAALADLAGATVEAALEVATRVVGGGEDGLLVDVLVVG
                     MGSLGGRELGYASDADVMVIHQPRPGVSDEAAHRQGLAIVQELVRLVAATGSDALVLD
                     TDLRPEGKNGPLTRSLASYAVYYDRWALTWEFQALLRARPIAGDRGLWEAFAALIDPL
                     RYPAAGLDATQVREIRTMKARVESERLPRGADPRTHLKLGRGGLTDVEWTVQLLQLRH
                     AHEVPSLQTPGTMAGLRAATAAGLVGEEDSATLAAAYENASRLRNANLLWRGRPGDAV
                     PTDIRDADGVGRIVGRDPGTGAGLMEDYLRASRRARAVTESLFYESQ"
BASE COUNT         5170 a        12142 c        11631 g         5073 t
ORIGIN      
        1 taacaacgac acgccggtgt caattgcgca aacgtgtcgc acggcatacc gacagccctc
       61 cccctggctt tgacagcgcg cccctgcccg tgggaacgtc acgtcaccga gacccgtcca
      121 cgggggaagc cggtgcaaaa ccggcgctgt cccgcaaccg tgacctctcg cccgacttcg
      181 gccggtgcgc aggggaagtc ggaacgcctg tggcggtgtc ggctccaaca catcgtccgt
      241 cgtggaaagc ggaccggagc gaagcgtcgg gcgacaagcc cggggcctcc caccggcccc
      301 gaggaggtcc ccacccatgc tgtctgtcac cccatccatc gctgctcgtc gctccgcgtc
      361 gcggcgcgca ttcgccgtcc tggccgccgt catggtcgcc ttcgccaccc tggtcagcac
      421 cgccccccag gcctcggccg ccaactacct ctactggagc tactggcagc agaccgccgg
      481 gaagtgggcc ttcagctcag ttggtgccga caaggccact ccgagcgacg gtgccgtcga
      541 gggctggcgt tgggcgatcg acgacggcag tggcagccgc cccccgcgcg tgctgccgac
      601 cttcgagcag ctttgcgcca gcaccccggc cgaggcgggc aagaagcggg tcggcctcgt
      661 cgtcgacttc ggccgcgacg tcgacggtga cggcaaggtc actcccccgt cgttggtcgc
      721 gacctgcgcc ctggttccca cggcagccac cggcgccgac ctcctggcca aggccggcgg
      781 cgtccgcacc gacaagggct tgatttgcgc gatcggcggc taccccgcca ccggctgcgc
      841 cgcccagttg gagacgctca ccgaggccca gaaggcaccc gacacggccc tggcccttcc
      901 gacggccgcg ccgacgacgg caccgacggc caccaccccg gtggcgacga cgaccaccgc
      961 cgccacgccc ccggcgaccg ccaccgcagc agtcgtcgcg acgagcacca cggccgctgc
     1021 tggcggtggg acctcgccgc tgctgtgggt cctgttgttc gtggtcgccg ccggcatcgc
     1081 gtatgccgtc gcccgccggc gtcgccccag cggagtctga ccggagcgac accacatggc
     1141 ccgccccaca cccgccgcca ccccccggat gctgcatccg gtggcgtggt gggtgtgggg
     1201 cgtcggcctg gcgaccgcag ccgcccacac gaccaacccc tggctgctcc tgctcgtcat
     1261 cgcggtcgcc ggttgggtcg tcctggaacg ccgtgaagtc ggcgcgctgg actcgttccg
     1321 gcccttcctc atcatcggcc tgtttgccat cgggctgcgg gtcgccctga cgatcctgct
     1381 gggcaacggc gtccccggcc aggtcgtcct tgtcgaactc ccccaggtcc ggctcccgga
     1441 ctggttcgcc gggctgcgcc tcggcggccc cgtgaccctc gagtccgttg tcgcggcagg
     1501 gatcgacggg cttcggctgg ccacgacgct ggcctgcctg ggggcggcca atgcgctggc
     1561 cagcccacga cggttgctgc gctacgcacc cgccaccctc tatgacatcg gcaccgccat
     1621 cgtcgttgcg ctcaccttcg cgcccgcgct cgtcgacctg gccggacggg tgcgttcggc
     1681 ccgacgcctt cggggccaca gtggaaccgg tgtccgagag ttggcccggc tgggcgtccc
     1741 cgtcctcgaa ggggctctgg accggtccct ggacctcgca gcatcgatgg agtcacgcgg
     1801 ctacggccgc accgccgtgc gtggtcgacg cgaacggatc gttggcagcg cactcgccct
     1861 cagtggcgca tgcggttcgg tcgccgggct cttcggcgtg atgtccgcgt cgtccggggg
     1921 atggtttggc ctgccgatgc tcgtcagcgg gctcgccctg ctggccacct cgctcgtcct
     1981 gggctcccgc agagacgcgc ggacggcata ccgacgcgac ccatggcgcg cgcccgaatg
     2041 ggcgacctgc gcgctcggtc tgcttccggc cgtgctcctc gtgcacgccg aggccgacgc
     2101 ctggccgggg atcaccatct cccaggtccc cctcgactgg ccgcccctgc ccgcgctccc
     2161 gaccctcgcg atcctcgcgg cggtgctggt ggcgtgggtg actcctgttc ctccccggct
     2221 ggctcgagcg cgagccacca cccttcccgc ccggctggag accgcatgat cgagcttcgc
     2281 ggcgtcggcg tcacgtatgc cggtgcgtcc cgtcccagcc tgcgtgacgt ggatctgcgc
     2341 atcgaggagg gcgaactcgt cctcatcgtc gggccgaccg gctcgggcaa gtcgaccctg
     2401 ttgcggaccc tcaacgggct ggtcccccac ttctccggcg gaaccctcac cggatcggtc
     2461 gtcgtcggcg gacgtgacac ccgggcgcac cgaccgcgag atctggccga cctggtcggc
     2521 ttcgtcgtgc aggacccgac ctccgccttc gtcaccgaca cggtcgagga cgagatcgcc
     2581 tacggaatgg agacgctcgg cgtcgagccg accgcgatgc gccggcggct cgaggagacc
     2641 ctcgacatca tgggcctggc cgagttgcgc gatcgcagcc tgcgggagct gtccggaggg
     2701 cagcagcaac gcgtctccat cgccgccgtg ctggccgccg gcccacgggt gctggtgctc
     2761 gacgagccga cctccgctct ggaccccgtg gctgccgaag aggtcctgtc ggcgctgcac
     2821 cggctcgtcc acgacctggg ttacaccgtc gtcctcgccg agcaccggct ggaacgggtc
     2881 gtgcagcacg ccgaccggat cgtcctggtc cgcgacggcg tcgtctccgc gccactcgac
     2941 ccggccgagg cgatggctct ctcccccgtg catccgccga tcgtcgagtt gggtcgacgg
     3001 atgggatggt caccgctgcc gctgacgatc cgcgacgccc ggcgtcgggc cggctcgctg
     3061 cgcgcccggc tggcggcttt ggacggcacg cctgcggcct ccgcacccgt caaggacgcc
     3121 gcctcgcgat cgctggtgtc ggtcaaccgg ctcgtcgtgc gccgcggaac cgtcgaggcc
     3181 ctctccaagg tcaccctcgg cgtgagcgcc gggtcggtca cggccgttat gggccgcaac
     3241 ggtgccggca agtcgaccct cctggccgcg atggccggtc agctcgccgc gacctcgggc
     3301 gaggtctccg tggccggctc gtcaccccgg cgcctcgccc cgcacgatcg ggtccgcgtc
     3361 gtcggtctgg tcccccagga ctccaacctg ctcttgtatg ccgattccgt ggccgccgag
     3421 tgccgggccg cagaccggga cttcgccgcg gcggacggga cgacgcgcgc gttgctcgcg
     3481 cggatcgcgg gcgaggtcga cggtgatcag cacccccggg acctgtccga aggccagcga
     3541 cttgctctgg cgctcgccat catcctcgcc ggacgccccc aggtcgtcct gctcgacgaa
     3601 ccgacgcggg gtcttgacta cccggccaaa cgccggctcg tcggcatcct gcgcgacctc
     3661 gccgccgagg gccatgccgt catcgtggca acccacgatg tcgagttggt cgccgaggtc
     3721 gcccagcggg tcatcgtcct ggctgagggt gaggttgtcg tcgacgggcc gatccacgag
     3781 gtgctcaccg gctcccccgc gttcgctccc caggtcgcca agatcctgca cccgctgccg
     3841 ctgctgaccg tcgacgacgt cagtgccgcc ctggtccccg cgtcgtgagg tttcgccccg
     3901 ccgctgccgc agcgctggcc ctcaccaccg tcgtcgggct cgcggcatac ctctggccgt
     3961 tcttcgccgc cccgggtttc tcgacgagct acggccagga cgcgccatgg ctgttcgcgg
     4021 ccctgctggg gttgatggcc ttcgtcgtcc tcgccgaggt caccgcgaac ggcctggacg
     4081 ccaagaccgt cgcggtgctc ggggtgcttg ccgccgcggg aggagccctg cgtgtcctgt
     4141 cggccggcac cgccgggctg gagccgatct tcgtcatctt cgtcctcggc ggccgggtcc
     4201 tcggtcgctc gatgggtttc ctcatggggt cgctggcgat gctcgccggg gccttcctca
     4261 ccgccggggt cggcccgtgg ctgccgttcc agatgctcgg cgccggatgg gtcgcgctcg
     4321 gtgcggcgat gctcccgcgg gccacggggc gagtggagct cgcgatgctc gccgggtacg
     4381 gcgctttcgc cgggctcgcc tacggcgtgc tgctcaacct gtggttctgg ccgttcctgg
     4441 ctcccgcatc cgcgcccacc ggtgccggtt tcgtgcccgg cgactcggcg gcggcgaact
     4501 tcgcccacta cgccgtcttc tacgtcgcca cctcgctcgg ctgggacgtc cctcgggcgg
     4561 tgctcaccgc cgtgttgatc ctcgttgccg gacgctcgat cctcgcgacc ctgcgccgag
     4621 cggtccgacg ggccgccttc ggcgaggtcg tccgcttcga ctagtcctcg cgagtctgca
     4681 cctggacgaa cggcgggttc atcaccgtgg cctccacccc gcgaccgcgc acgtcgatga
     4741 ggacctcggc gtccaacggc accgaacgat ccagcaacgc gagggcgatc ccctgcttgc
     4801 gggtcggcga gaacgtcccc gacgtcacct ccccgatcac ctcaccatcg agcgtgaccg
     4861 ggcagtgcgc ccgaggtatg ccgcgtccgg tcaccaggag cccgcgcgag atccgcgacg
     4921 tgggcgcggc cttctcggcc agcagcgcct cccgacccca gaaggccggc ttggcccagc
     4981 ccaccgccca gcccgaccgg gccatgacag gggtgatatc caacgacagg tcctgaccgt
     5041 gaagcggata acccatctcg gtccgcaggg tgtcgcgtgc gccgaggcca cacggcatac
     5101 ctccccaggg gaccatcgcc tcgatgatcg cgtcccacag gtcggctgcg tcgtcccacc
     5161 gcgggaccaa ctcgtagccg cgttcgccgg tgtagccggt gcgacagacg atcacgggac
     5221 gaccctgcca gtcggcctca cggaactgca tgtagtcgtg tccggtcggc aagccgagcg
     5281 catcgagcac ctcgtccgat cggggcccct gaacggcgat cacggcatag tcccggtgcc
     5341 tgtcgaccac ctcgatgccc tcgggagccg cggcctgcag ccgacggcat acctctgcgg
     5401 tgttcgcggc gttggggatg aggaagacgt cgtcgtcggc ccgcaggtat tggatgaggt
     5461 cgtcgacgac cccgcccgtg gcgtcgcagc acagcgtgta ctgcgcctga cccggaccga
     5521 cccggcgcag gtcgttggtg aagcacgcgt tgacgaagtc ggccgcaccc ggcccggaca
     5581 ccgtggcttt gcccaggtgg ctcacgtcga agaccccgac ccgctcgcga accgcggtgt
     5641 gttcggcgac cactccgccg ccggggtatt cgatgggcat ctcccaccca ccgaagtcgg
     5701 ccagtttggc ccccagagca acgtgacggt cgtgcagcgg tgagtgcgcg agatcggcca
     5761 tggccaacga gcctaacggg ccggacccgg ggccgtcatg gccctggccg ctcgcgtgca
     5821 tcaagaggtt ggcctagccg ccagacttgc gccggaacat gcgctgcgcc gcggccgggc
     5881 cgtgagcgtc gtggaccttg ccttcgccct cgcccgcact ctcgcggccg gcatgggcct
     5941 gcttgcgctc gagggcctcg cggaacttgg ccttcatctc gtccgagggg ccggcggcct
     6001 tgggggcctt accggacttg gcgttcttgc tcatgctcgt tcgtccttcc gtcagtcgtc
     6061 cgaccgggaa cccggtcggg tgtcgccgcg cttgtctcgg cgctggcgtg aactccattc
     6121 gcgcatccgg ctgggatagc cggtgaggag gacgtcgtaa atcgggatcc ccagagcggc
     6181 cgccatctca taggccgccg cacgcgaccc gacggcccgc cgagtccact ccccgtcggt
     6241 cgcaacgagc accacgcttt gggtgctgac gttggtggcc ggctcgagaa atgcctcgac
     6301 cccgcgtcgg gtttggacga attgggcgaa gtgcccgcgc acggtcgcca gatcgacgcg
     6361 catcgccggc ccgtcggatc gacccaggtc aggcagcgac gaccgacgcc atcggtcgaa
     6421 gagtcccata gtccgaacct tatgctgtcc gcgtgagcac tcggggcacc tccacagggc
     6481 gatgggcggg tgtcggcgtc gctggagtca tcatcctcag cctggccagc atcggggcgc
     6541 atgcccttct cattcctggc ttcatccagg cagcagacgc gtcctcacgc tcgaccggcg
     6601 cactgtccac cccgacgact gtcccgtccg ggcagggcgg cacggggtcc ctcagctcga
     6661 ccagttcgcc cgccgcgacc gatctggcgc cgacggcggt gccggccatg ccggcatacg
     6721 accgggcctg cctgcgccgc gacaaccccg ccacgggagc gaagatcccc gcgaacgatc
     6781 cgttcgggga cgacatcgcc gacctgggcg acaaggccga gttgcagcgc ctgtatgccg
     6841 tgcagagcgg agcgctcgtc ccgctcgacg gcctcggggc gccacgaccg tgcgacgagc
     6901 agttgtggtc gctcgtcaag gccaccgccc ccaccctgct cggccacatc gacgagttgc
     6961 tcgtcttcga tgccgacccc gatccggcga cgggcgagtt catcatcgag ggcgagtcgg
     7021 cccccaagga gacggcgccc gacacgttcg acgacgacca ctggcgcgtg tcgttcgcac
     7081 ccaacgggtt ggaccgcggc gagctcgcct ggctggttgc gcacgagctc gcccatgtcg
     7141 ccagcctcaa caaggaccag atgctcagcg gcgtcggtgc cgacgtctgc gccacctggt
     7201 acaccggcac cggctgcctg ctggcggact cgttcctgcg gcgttacctc agcaacacat
     7261 gggacgacgc cctctgggat gactgggata aggccgacgg caagtccacc gaaaaggcgc
     7321 ggcgcgcggc ataccaggac ttctatgaca accacagcga ttccttcatc accgagtacg
     7381 ccgcctggca ccccctggag gacttcgcgg agtccttcgc catgtggtgc acctacgacg
     7441 aggacgagcc ggaccgggct aacctgccga ccgcagaacg gaccgacagc ggaaacaagg
     7501 ttgcctggtt cgatgccgca cggcgcgatc tcctgcccgc cttcgggccc ggctgcacga
     7561 tgctgcgaca gttcgccgtc agctgatcgc cacggcgcgt ccacccgaca cagttgtgac
     7621 acggcatcgc gcgaagtgac aagatgcggg tggaccttcg cacacggaca ggagcccaga
     7681 tgacggacga ggcgcccgat atctacgacg tcctcgtcct cggcggcggt agcggcggat
     7741 atgcctgtgc gttgcgctcc gcccaactgg gattgcgggt tgcccttatc gagcgcgaca
     7801 agctcggcgg cacctgcctg caccgcgggt gcatccccac caaagccctg ctgcacgccg
     7861 ctgaagtggc cgacacggcc cgcgaaggcg cccggttcgg ggtcaactcc agcgttgagt
     7921 cgatcgacat gtcggccgtc ctgcgctatc agcagggagt cgtgtcccgc ctgcacaagg
     7981 gtctgcaggg gctgatctca gcggcgacca tcgactacgt cgacgggttc ggcgaggtca
     8041 cctcgcccac gacggtccgt gtcggcgacc gcaccctcac gggccggcgc ctcgtcctgg
     8101 ccacggggtc gcgagcccgc tcgttgcctg ggctggagat cggcggccgg gtgctcacca
     8161 gcgaccaggc cctcggcctc acgcaggtgc cgacctcggt cattgtcctc gggggcggcg
     8221 tgatcggagt cgagttcgcc tccgttttcc gctccttcgg cgcgagcgtg acgatcatcg
     8281 aggccctgcc gcggctggtg ccggctgagg aggaaagcat ctccaaggcc ctcgaacggg
     8341 ccttccgcaa gcgcggcatc gtcagccgca cgaatgcacg attcgcctcc gtggcccagg
     8401 acgacgccag cgtcacggtc agcctcgagg acggctccac cctgtcggca gacctgctgc
     8461 tcgtcgccgt gggccgcgaa ccggtctccg acggcatggg tttccaggag gcgggagtcc
     8521 acaccgaccg gggtttcgtt ccgaccgacg agcgcctgcg caccaatatc cccggggtgt
     8581 atgccgtggg cgacctcgtc cccggtctgc agttggcgca tcgcggcttc gcccacggag
     8641 tgttcgtcgc cgaggacatt gccagcttga accccgcccc cgtgctggac cgcaacatcc
     8701 ccaaggtcac ctactgcgac cccgagatcg ccagcgtcgg cctcacagaa gcagccgccc
     8761 gcgcagagca cggcgaggtc cgcgcttacg agtacaacct cggcggcaac ggcaagtccc
     8821 agatcctcgg gaccagcgga ttcgtgtccg tggtgagagt ggtcgacggg ccggtggtcg
     8881 gtatccatat gatcggggct cggatgggtg agcaaatcgg cgaggcacaa ctcgtcgtcg
     8941 gatgggacgc catgcctgaa gacgtggccc cactcgtgca cgcccacccg acccagaacg
     9001 aagctctcgg cgaggcccat ctggcgctgg cgggtaaacc cttgcacgcc cacgcctgac
     9061 cgcaccgaga accttttgca gcgcagacag attgacgacc gagaaccatc gacgaggaga
     9121 tgacgtagat gtccaatcgc gtgaccatgc cggccctggg tgaatccgtc accgagggga
     9181 cggtcacccg ttggctgaag aaggtcggtg acccgatcgc catcgacgag ccgctcgtcg
     9241 aagtgtcgac cgacaaggtc gacaccgaga tcccgtcccc cttcgcgggc atcgtcgagg
     9301 cgatcctcgt cgccgaggac gaggtcgccg ccgtcggagc cgacctcgca gtcatcggcg
     9361 acggttctgg ggccgcggct gcgccggcgg cgcctgccgt cgaagctgct cccgctcctg
     9421 ctccggctgt cgctcccgct cctgccccgg agccggccgc ggtggtcgtc gctgagcctg
     9481 cgcccgcgcc tgcccccgct gcggccgagg cgtccggcga tggccacgag gtcaccatgc
     9541 ccgcgctcgg cgaatccgtg accgaaggca ccgtgactcg ctggctgaag aaggaaggcg
     9601 acacggtcgc cgtcgacgag ccgctcttgg aggtgtcgac cgacaaggtc gacaccgaga
     9661 tccccagccc gttcgccgga gtcctgagcc gcatcctcgt cggtgaggac gaggtggccg
     9721 ccgtcggcgc tccgttggcg atcatcggcg gctcggcctc cgcgcccgct ccggccgctg
     9781 cgcctgctgc cgccccggct cccgtcgctg ccccggcacc tgtcgctgcc ccggctccgc
     9841 agacagtcgc gccggctgcc cccgcacccg ccgctgcccc cgcaccggct gcttccggcg
     9901 acgagggtga ctccgacggc gaaggcgcca gctacgtcac cccgctggtc cgcaagctcg
     9961 ctgcgctgca caaggtcgac ctggcttccc tcgtcggcac cggcgtcggt gggcggatcc
    10021 gcaagcagga tgtgctcgac gccgccgagg ccgctcgcaa cgctccggcc gcacccgccc
    10081 ccgttccggc tccggctgcc ccggctcctg ctgccgctgc ccccgcccca gctcccgctg
    10141 ccccagctcc tgttgccgca gcgcccgctg ccgcggcccc tgctgcttcg gctgcgccgg
    10201 catcggcacg cccgtcgctg cagccgtcct cgaagcgcgg caccaccgag aagatgtcgc
    10261 ggttgcgcaa ggtcatcgcc tcgcgcatgg tcgagtcgct gcaggtctcg gcacagctca
    10321 cgaccgtcgt tgaggtcgac ctcacccgcg tcgcccggct ccgggcacgt tcgaaggccg
    10381 ccttccaggc ccgcgagggc accagcctgt cctacctgcc cttcctcgcc ctcgccgcgg
    10441 tggaggccct caaggtccac ccgatggtca acgcgagcat caacggtgac gaggtcattt
    10501 accacggtca ggagcacctg tcgatcgccg tcgacaccga ccgtggcctc atcgtccccg
    10561 tcgtcaagaa cgccggtgac ctcaacatcg ccggtctcgc ccgggggatc gccgacgtgg
    10621 cgaatcggac ccgcaccaac aagatcacgc ccgatgacct caccggtggc accttcacga
    10681 tcaccaacac cgggagccgt ggggccctgt tcgacacccc gatcatcaac cagccgcagg
    10741 tggccatcct cggcaccggg gccttggtga agcgtccggt tgtggtgagc gatgcagagg
    10801 gcggcgagac gattgccgtc cgctcgatga tgtatctcgc cttgtcctac gaccaccgga
    10861 tcgtcgatgg cgccgacgcg gcccgcttcc tcgagacgat gaagcaccgg ttggaagagg
    10921 gcaacttcga gcccgacctg ggcctgtaag gaacgtgtgg tgagcggtcg gcgggttgcc
    10981 gtcaccggcg cttccggttt catcggcggc gcgttgacgg catacctcgc cggtcgtggc
    11041 gacgaggtcg tgcggctggt ccggcatcag ccgacgcagc cgtcggagcg ccggtgggac
    11101 ccggtgcgcg gaggcctcga cccgcgggcg atcgacgacg tcgacgcgat cgtccatctc
    11161 gccgcggcgg gtgtcggtga tcaccgctgg acccccgcct tcaaggaact cgtgctccgc
    11221 tcgcgcgtcg acggaaccgc cgcggtcgct acggcgatcg cccagacggg tcggccgatc
    11281 cggttcgtgt gcggatcggc gatcggctac tacggcgatc gccgcgatga gatcctcact
    11341 gagctcagcg gcccggggac cgggttcctc gccgaggtcg cccgggcctg ggagggctcc
    11401 gccgaaccag caatcgctgc cggcgcaccc gtcgctttcg cccgccacgg gctggtcatg
    11461 ggtccagggg gtggcgcgtt cgcccgcctc ttgcgcctgg ctcgctgggg gctcttcggc
    11521 ccgctggggt cgggacgtca gtactggtca tggatcaccc tggctgacag cgtccgggcg
    11581 ctcagtcatc tcatcgaccg acccgacctc accggcccgg tcaacatcgt tggccccgac
    11641 cctcgccccc aacgggttgt ggcgagcgcg atcggtcacg ccctgcatcg gccggcgatg
    11701 ctcccggccc cgtcggtggc cctgcgggtg gcgctcggcg agttcgcgga cgacatcctg
    11761 ggctcgcagc gggtgatgcc gttcgcgctc accggaagcg gattcacctt cgaacactcg
    11821 atgttgcgcc cggccgccgc atggctggtc cgcgaatcct cgtgactcgc tgacctgcca
    11881 ccctctttga tctgccggcc actcccacgg tcatcccgtg acagttgaga tccgccattc
    11941 ctcaccaacc cgcaccagcg agtagagcag cgatcggcca ctgtcggccc cgatggattg
    12001 ttcgcgatca cccggaccct gcacgcgata cccgctgcgt tcgacgacgg cccggatgcg
    12061 cgctgacccg gcatccacgc tgacccattc ggcgacggtg accgtgaagg tcagcccgac
    12121 atagcgctgc tcggcctgaa ccaactcggc gacgatggcc tcatcggcgg ccagcaacgt
    12181 ggaagcctgc ccgtcagcgg ccgcgagcgc ccgcaggtct cgggcggtca gcgcctgggc
    12241 ccggcggccg accagggtgg ccagcaccgc cctggcctca cccgctggtg ccgccgcgga
    12301 gcccagcacc cgagtgacct ctcctgccga accctccgcc gcggcggccg gactcaagcg
    12361 gaggaacacg ctgacgacga ctgcggccag cacgatggcc agggctgctc cgagacccag
    12421 ccgggccttg cgccccaccc tcggacccgg caccctgccg ccggcccggc ccctgcgcgc
    12481 tgcctggccc cggcgcactg cctggctcct gcccggacct gccccgacgc ccggtgtcac
    12541 ccgacccgcc gcgcctcgca ggcgttgggt gaggacccgg tcaggatcgg cacccgccgc
    12601 cgaacccacc aggcgaacgg ccaccgggct ggcggcttga taggccagca cggcggcctc
    12661 ctgggcctcc gggcgaccgg acggatcttg cgagagcatc gccgccacca ccggcccgaa
    12721 cccggcaccc agcactgcca cagcttcatc caccgtcacc gggcccggca ccgacgtgtc
    12781 cgcgccaccc aacggcgttg cccaggaccg gaccgaccag gatcggccgg tcaacgcaaa
    12841 ccacgccagc gcccccaggc cgaagacgtc cgcggcctca ccgggcacat ctcccgcgac
    12901 cacctccgga gcgacgaaac ccgaggtccc agccacgagc ggcgggtgac cctcccccac
    12961 tagccgggct gccccaaagt cgctcagcag cggtttcccg tcagccgtga acaggacgtt
    13021 ccccggcgag agatcgccgt gaacgacgcc ggctgcgtgc agggcggcca gggcgccggc
    13081 gagcggtgtg cagatcgtcg ccacctcacc gccatccagg cgcccacggg ccgccaccag
    13141 atcggcaagg ctgccccccg gtgccagatc aaggacgacc gcgaccgacc cgtccgggag
    13201 ggcatgggcc tcgtgcaacc gcacgaggtg atcgtgctcg acggtggcca ggatggcggt
    13261 ctcccgggcc gccaactcca tgagttggcc gacgtgcaac cgatcgggcg acgtgatctt
    13321 gacggccacg gcccgcccgt catcgacccg gacggccgac cacacggtgc tcgtggcccc
    13381 gcgcccgatg acctggccga ggaccagccc gggaaccgag ggtggggacg tcttggcagc
    13441 gctcacccac tcatggtgcc gctgacgagc gatcctccgc agaagttatc cacagccctg
    13501 ctggcacgct tggacccagg tgacaccccg gcctcgccca tccatctggc gtgcgatcag
    13561 tgcgggtctt cgccgggggt gatgacgtcg acgacccggc cgtcggcggc gctgatccgg
    13621 gccgcgtcga tgacggccag cacgtggacc gcgtcgcggg ggtcgaccgg catactctcc
    13681 tgcgggtccg gcgacgcgag ggctgctgcc acctcgcggt agaagtcggc cgggtcgctc
    13741 gagcgggcga ggaccgcctc ccgctcgtcg ccacgcacga gccagccgtg ttggcccggg
    13801 ccgccgtcgg cgtcgtggaa cgcggtgggc tcgccgccgg ccgagccgag caggaacgtc
    13861 ccggcgctcc ccgtgatccg agcccgcggg cccggggctc ccgccacact gctggcgctc
    13921 aggtgtgtgg agtagccgtc ggtatgccga agcgcgacga aggtgtcgtc ttccgcggtc
    13981 accgtccagg ctgcgagctc ggcatatacc gtcgccaccg gcccgtgcat gaggacaacc
    14041 tggtcgagca ggtgcgtgtg cagatcgagc agtaagccgc cgccctcggc cgccgtggcg
    14101 cgctcgcgcc agcggtcctt ggggactggc cgccagcgat cccagcgcat ctcggtgcga
    14161 cgcagttcgc cgagaacgcc gcgatgcagc aggtcgcgga tcgtcgcgaa ctccgggtcg
    14221 tagcgacggt tctggaacac cgtcagaggc acgccgcgac ccgcagccag gtcgaccaaa
    14281 ccctgggctt ggtgggcgtc ggtggcaatc ggcttgtcga cgacgacagc gatgccggct
    14341 tcgaggagcg cctcggcatg ctcgacgtgg cgaccgctcg gggacgccac gacgacgaca
    14401 tcgaggtcgg gcacggccac cagcgcggcc aggtcaggca cgacgacgac gccggggttc
    14461 tcggcccgcg cctggtctgc gcgctccggg ttgcccgtga cgacggccac aaggtccagc
    14521 cccgcctccc gcagcagcgg cgcatggaag cccgcacccg acgagccgta tcccaccagt
    14581 ccaacgcgag tcatcacgtg cctcccttcc cgccgcggcg gcgaggtgcc caccctaccg
    14641 aggcgggcgg catacaccct cgggtatgcc gtcgcgccgg gttaaggtgg gcctcatgcg
    14701 cgtcgtccac ctcggcttcg atcccgactt cgtggactac gaatgggcgt gggcgcatca
    14761 gcgcgaggtg cacgcgaagg tcgtgtccgg ggaggaaccc gacacggtgc tgctcctcga
    14821 acaccaaccc gtctacacgg cagggaagcg caccgaggcg cacgagcgac cgaacgacgg
    14881 cacaccggtc gtcgatgtcg accgaggcgg caagatcacc tggcacgggc cgggtcaggt
    14941 caccggatac ccgatcgtcc gcctgccggc cccaatcgat gtcgtcgcct atgtgcgact
    15001 ccttgaggag atgatgatcc gggtctgcgc cgacttcggg gtgtcggctg ggcgcgtgga
    15061 cgggcagtcg gggacgtgga tcctcgccga ccccgcagat ccccggcgtc ggccgaaccg
    15121 caagatcggc gccatcggca tccgggtcag tcgtcaggtc gcgatgcatg ggttcgcgtt
    15181 caacgccaac ccgtcgctgg cgtgggccga cgtcatcatc ccctgcggca tccccgacgc
    15241 cggggtcacg tcgctgtcag ccgaagtggg ccgtgacgtg ccggtccgcg aggtcctccc
    15301 ccacatcgag aagcaccttg ccgacgtgct gggcacgttg gcgccaaacc gtcgggcgat
    15361 ctgagctcac gccggacgta aactgggaga cccgcggacg gcatactcct gacccgttgc
    15421 ggcaggaagg acggacccgg tgacgatcgc acccgagggg cggcggatgt tgcgcgtcga
    15481 ggcgcgcaac gcgcagaccc cgatcgaacg caagcccgaa tggatccgca cgacggccaa
    15541 gatgggcccg gccttcaccg aactgcattc gatggtcaag ggcgagggct tgcacaccgt
    15601 gtgccaggag gccgcctgcc ccaacatctt cgagtgttgg gaagaccgtg aggcgacctt
    15661 cctcatcggc ggcgacacct gcacgcggcg atgcgacttc tgtgacatcg cgaccggtcg
    15721 cccgcagcca ctcgaccccg acgagccgcg ccgggtggcc gagtcggtgc gccggatggg
    15781 gctgcgctac agcacggtga ccggtgtcgc gcgcgacgac cagccggatg gggcggccgg
    15841 cctgtatgcc gcgacgatcc gcgcgatcca cgaactcaac ccgaacaccg gtgtggagat
    15901 cctgcccccg gacttcggcg ccaagccgga gttggtcggc caggtcttcg acgcccggcc
    15961 ggaggtgttc gcgcacaatc tggagacggt gccgcggatc ttcaagcgga tccgtccggc
    16021 gttcacctac gacacgtcgc tgcgggtgtt gtcgatggcc cgcgaggctg gtctggtcac
    16081 caagagcaac ctcatcctcg ggatgggcga ggaggcgcag gagatcgagg cggccatcgt
    16141 cgacctgcat gacgccgggt gcgacatcct cacgatcacg cagtatctgc gtccgtcggc
    16201 caagcatcac cccatcgacc ggtgggtcaa gcccgaagag ttcctgcatt ggtcgtcggt
    16261 cgccaccgag cacggcttca agggcgtcat ggcggggccg ttggtgcgct cgtcgtatcg
    16321 cgcggggcgg ctgtgggcgc aggcgatggc gcggtggggt cggccggttc ccgagcattt
    16381 ggcgcacctg gcggaggctg ccggagcccc ggcgcgtcag gaggccgtag ccgtggtcgc
    16441 ggcattcgca gagcgctccg gcgcccccgt atcctgactc ccatggccaa ggacccctcc
    16501 agcaccgccg ccccgaagaa gcccgggcgg atcgctcaac tgcgcaccgt cctgcagcag
    16561 tccaaggcgc tcgatccgaa gatcgtctgg tggatggcgg gggcgttcgt cctcacgctc
    16621 gcggtcattg tcggcatcgg gtggctggtc aactggccgg tgtatgccgc aatcctcggc
    16681 ctcccgttgg ctgccctggc cgccaccatc gtcatgagcc gccgagccga gggggcggca
    16741 tacgcgaagc tcgaggggca gcctggagcc gcgggtgcgg cgctgacgtc gctgcgtggc
    16801 gggtggttca cctcgtccga gccggtggcc gtcgagggtg cccggtcagg gagcatggcc
    16861 gacgccgccc tcgtctatcg cgcggtcggg cgcccgggtg tcgtgctggt ggccgagggg
    16921 cctgaggcgc gggcgcagcg gctgctgctg gctgagaaga agcgcaccga gcgggtggcg
    16981 cctggggttc cggtgacggt ctatcgggtc ggttcgggta aggaggaggg ggtcgtcgag
    17041 atccgcaagc tcacctcgaa ggtgcagcgg atgcgcgcga cgctctcgaa ggacgacgtc
    17101 ctcaaggtca accagcggct gcgggctctg ggcggcgtga agctgccggt gccgcagggc
    17161 atcgacccgg cgcgggcacg cccggaccgc aagggtatgc gcggccggtg aggatctagc
    17221 gagcgaggaa cgagcgggcg ctgacgagcc ggcccgtagt ggcggccggt gacgaggtga
    17281 gcgcagcgaa gcaagcggcg aggaaccggc ctatgtgacg gccggtgagg atctagcgag
    17341 tcggccgagc gaggatctag cgagcgagga acgagcgagc gctgacgagc cggcaatgtc
    17401 accgagggcg aggaaccggc ctatgtgaca gccggtgagg atctagcgag tcggccggtg
    17461 aggatctagc ggcccgctga ggacgccgct tactcggcgg cggtgtcgtc ctgccagggg
    17521 tggcgctcgg gtcggaagag ttggaccgat cccacgggga cgaagccgac gtgcaggaag
    17581 agccgcaggg accgggcatt gccgggggcg acggcggcga cgagcgggtc agagtgcccg
    17641 agttcgtcat cgacccaccg cagcgcggcg tcgagcaact cgcgccccga tgtcggccct
    17701 tcggtctgca gcccgatctc gggtagtcca cccagccccg acgcgacggt gacgaccgag
    17761 cggacccggc gggacggcat accgaggact ctgatgccgc tgcggacgtg ctgggcgacg
    17821 gcgacccgcg ggtgccctgc caggtcgtcc ctggacacga ggtccgggcc ggtcgcggtg
    17881 gggcgggggg cgtccggagg gcggacgagc aggacgtcga gggcgtccgc ccaacccgat
    17941 ccgcggatga gctcggcgac ccgcgggtgg tgtgcgccgc cgtagccgtc gacgccgtag
    18001 gcgtccaact cgacgtcggc aaggtcctgg cccaccgcga ggtaggcgtg cccagtcatg
    18061 gcaacgaccg cctcgactcc gcgcaaccag agcgaggctc gttcccagcc tccgtcggcc
    18121 gccgggaact ctcccttggc cgccgaccgc aggacgtctg cgaggtcggc cggcgagccg
    18181 ctcaccggcg cacgagcacg gtcgccgccg ccttgtcgtg cagaccgcga ctatcgcggt
    18241 cccagatgag ggcgggcacg gcaaggcaca gcagcagcgt ccgcagggtc gcttggacga
    18301 ccgagatccg gcgtcggtcg acggtcatga tgacgatccc gagcagccgc tgaccgacgg
    18361 tggcgccgag ggtgggcagc agcaagagat tctcgacggc gaagacggcc agcggagcga
    18421 aactgccagg gccgccctgc ccgagctcgg cgcggaacac gaagacggcg atgagttggc
    18481 acaacgccca gtcgatgcag attgcgacga gtcggcgccc gaaccgggcg atcgatccgg
    18541 ggccggatgg cggcataccc aggtcttgtc cggggtactc gcctggagcc tgagtgcggg
    18601 cccggggccc ttccaaccag gaggcgacgt cacgacgatc gaccaccccg atagcctagt
    18661 ccgcagcaca gcgtcgtgcg tgcacgcacc cgggctccag ttaacagggg cgaaacatca
    18721 gcaacacgac cgggaaatcc gtccgcccta gcgtcggggc gaagcaccac accgggcgtc
    18781 gcattgcgtt gtgtcctgcg gcgatccggg tccactctgg aggaggttgg tttgttcact
    18841 tcagccgacg aggttctggc ctatatcaag gccgagaacg tcaagttcgt cgacatccgg
    18901 ttctgtgacc tgcccggggt catgcagcac ttcaacgttc cggcgcacac gctggacgag
    18961 gacttcttca ccgtcggcca ggctttcgac ggcagctcga tccgcgggtt ccaagcgatc
    19021 cacgagtcgg acatgaagct cctgcccgac gtggcgacgg catacctcga cccgttccgc
    19081 gtcgagaaga ccctggtcat caacttctcg atcgtcgacc cgttcaccga cgagccctac
    19141 agccgtgacc cgcgcaacat cgcggcgaag gcggaggcct acctcaagtc aacggggatc
    19201 gccgacacgg ccttcttcgg cgccgaggcg gagttcttcg tgttcgacga cgtgcggttc
    19261 aagacgtcgg ccaacgagag ctactactac gtcgactcca aggccgccgt gtggaacacc
    19321 ggcaaggtcg aggagggtgg caacctgggc tacaagccgc gcatgaaggg gggctacttc
    19381 ccggtgtcgc cggtcgacca cttcgccgac ctgcgtgacg agatgtgcct gcggctggcc
    19441 gaggtcggca tggaggtcga gcgttcgcac cacgaggtcg cctcgggtgg gcagcaggag
    19501 atcaactacc gcttcaacac gctgctgcac tccggcgacg acatgatgaa gttcaagtac
    19561 gtcatcaaga acaccgcctg ggccaacggc aagaccgtga ccttcatgcc gaagccgatc
    19621 ttcggcgaca acggctccgg gatgcacacg caccagtcgt tgtggaagga cggcaagccg
    19681 ctgttctacg acgagagcgg ctacggcggc ctctcggacc tggcccgctg gtacatcggt
    19741 ggtctgctca agcacgctcc ggcgctgctg gccttcacca acccgacggt caactcctac
    19801 caccgcctgg tgccgggcta tgaggctccg gtcaacctgg tctactcggc ccgtaaccgt
    19861 tcggcctgca tccgcatccc gatcgccggg gcgtcgccga aggccaagcg catcgagttc
    19921 cgcatcccgg acccctcgtg caacccgtac ctcgccttcg cggctcagct gatggcgggc
    19981 ctggacggca tcaagaaccg catcgagccc ccggagccgg tcgacaagga cctctacgag
    20041 ctgcccccgg aggagcacgc cgagatcaag caggtccccg gctcgctgcc cgaggtgctc
    20101 agcgccctgg aggtcgacca cgacttcctg cttgagggtg gcgtgttcac caaggacctc
    20161 atcgagacgt ggatcgagtg gaagcgcaag aacgaggtcg acccgatccg cttccgcccg
    20221 cacccccacg agttcgagat gtacttcgat atctgatagt cgctctgaac tgcggaatcg
    20281 tgctgtagcc ccgcagtgag aagtctggat caggatcacc aggtatgccg cgcccctcac
    20341 cgggagcgcg gcatacctgc gtttgcgggg tatgccgtgc acggccgccc gcgtgcgcca
    20401 cggagtgcgt ctggtggggt gatcaccccc tcgatcgcac tccgtcacac cctcgggagc
    20461 ctcagccccc acaaatgacc gcgcgagcgc tatctccggc ccccactgcg ccccgcgccc
    20521 ccacaaatga ccgtgcgagc gctatctccg ggtcaggagc gcgatgcggg tgccgcggcg
    20581 accgacgtac cgaccctaga ggccggcgtc gcgggctgcg ccgcaggtgc acatcccgac
    20641 gaacacgtcg tcggtcatga tcgtcgcctg acgagcgacc tctcgcagcg tcacctcgcc
    20701 ccactcgggc aattcggcaa tcagcccgag ggtgtccaac agcgccgcgg ccgacacctg
    20761 gtcgtgctga gcccggtcga cgtcctgcag ggcgagtcgg gcagcaacgg cgatggcgtg
    20821 tcggaaccag ttcaccgggt cgagcgcaca ccctggcgca tgccgcacca ctgatccgtc
    20881 catgggccca ttgtgccgat tcacccggtt tctctcgacg tgcgccgacg cgatggctag
    20941 gctcgccccg tctcccccgg cgcgcttcgg cccgccatcc gtttcgcacg ccgaggtctc
    21001 tcccccatgt caccgacgcc atccggcctg ttccacgatg tcccgattga ggacctgttc
    21061 ttctcgacga ctgacgccaa aggcgtcatc gacgaggcca acgaagtctt cgcccgcaac
    21121 tcccgctatg agcgaagtga gctcctcgga gcgccccaca acatcatccg ccaccccgac
    21181 atgcctggtg ccgtcttcaa ggccacctgg gatctgctcg gcgcgggcca tccagtgtgc
    21241 gcgtacgtca agaacctcgc gcgcgacggc agcacctatt gggcgttcgc gaccatcgtt
    21301 cccatcgcag ggaggttcct ctcggtgcga gcaacgccgt gcgaccggac cgcaagggac
    21361 ctgttcgagt cgctctatgc cacggtgcgc gaagccgaga tcgcagcccg ggacgccggt
    21421 gccagcgccc gcgaagcggc cgacgtgggc agtgaggtgc tcgggaaagc gctcgcggag
    21481 aacggtttcg gctcctacac cgaactccaa ctggatctcg tcccggtcga ggtgacggcc
    21541 cgcgaacagg tctgtccccc gttgccgcag ttgcctgacg ggtccaagac ggcacgcaag
    21601 atcctcgccc aggtcaccgc ggcacgcagt tcactcgggg ccttcgccgc cgatatcgcc
    21661 gattcgctca cgctgaccga caccctgtct cgcgacctgc gccgcttggg ggccgccctc
    21721 gccgggctgg atcgcgcggt ggccagtgca gcccagactc tgaccgcagc cacgcctgcc
    21781 atggcgaccg ggtccacgat ggtgacctac gcgtccgacc cggtggccga ttcggcagac
    21841 atgaccgttg gggccgtggg ggcggttggg accgttgagg ccgacgtggc cgcactggtg
    21901 cccatcatcc agagccgact cggctcgttg cgcgacgacg tcgagcgcgc aggcgacgaa
    21961 ctggcttccg tggtccgggc ccgcaagcac cttgcgctcg gcagcgcgat cgctcgcatg
    22021 caggcggagg cgatcgggcg atacgtggtc gctgtcgagg ccggcgccga ggacccgcgc
    22081 gtgtccgagc gcgcgctgtc gagtctgagc acggcactgc tggccatcct tgatgccgac
    22141 ctgctgaccg atcgagaggc gaccactgcc tatgtcgatc gggtggctcg gctggcgacc
    22201 gaggtggccg gggcgcgcga gacgaccgtc gcgtggcgct ccctcctcga gagcaccatg
    22261 ccgagggctg ccaccaacga cgggtcggtt accgacgacg ggtcggttac cgacgacggg
    22321 tcggaggctg gcaacgggtc ggtcaccggc gaccgccccg aagacggctc cgacgaccac
    22381 tccgacgccg cccacgagtc tcgactggcc ttcgacgtcg ccctaagtgc agcaaatgat
    22441 cagatgggcc gagtcgccga cgccgtcggt gccgtggctg cgacgaacgc cgcgctggac
    22501 cgtgacgagt tggctgccgt gctgggcaag atcgtcgagt tggccgccaa gatctagctc
    22561 ggtcgaggta ttccgccatc agcaggtcat ggcggaatac ctcgacccgg caaagggcgg
    22621 cgaagaggga tcagcggcgg ttgacggcga cgagcccgag gcagacgccg acgagcgcaa
    22681 ccacgatgcg gggactgacc ggctcgcgca gcagcacggc gccaccgacg agcgcgacga
    22741 ccggggtcaa cagcgtgaag gccgacagtt tcgtcgcgga gtaggtccgg atgagccaaa
    22801 accacacgac gaagctcgtg gagccgacga cgacgacctg gaacaccagg gccgcggcga
    22861 ccgtcatgga cagcggccac tgcgctcgtt ccccgacgaa ccagccgatg gcggtcaggc
    22921 cgaccccaga aacgccaagc tgatagagca gggtcttggc cgggtggatg ggcgcgagag
    22981 ccgtcgcacg gatcaccagc gtcgtcgctc cccacccgag tgcggcgagc ccggctagca
    23041 ggtcaccgag ccactgctgt gatccggcgc cggccgaggt gaagctatcg gcgaacgcga
    23101 agcccacacc ggcgaaggcc gcgatcaggc cggccagggc cagcccagtg agccgctcgg
    23161 cgcgcgcgat gaagggcatg ccgatggcga cgacgaacgg cgccgtgtag agaaacaccg
    23221 tcatccgccc cgcggtcgtg aattgcaggg ccgaatacac ggcggcgaac tcggccgcaa
    23281 acagggctcc ggccacgagc cccggcccga gcgtgcggtc ccgttcccac aggggtatgc
    23341 cgcgcagctt cgcccaggcg agcaccaaca gggccgcgcc gatcgagcgg agccccgctt
    23401 gggtcagcgg tggcacctcg tccagggcca ccttggaagc cacctggccc aggccccaga
    23461 cgaccgtgca cccgatgacg agcgacaccg ccagccgatc gaggtgcacc ctgcggtcgc
    23521 cgctcaccga ggcaccctat ccggccgcga cgtgggtatg ccgccctgcc gtcccgcggc
    23581 actcacagcc cgagccgcac cagccaagcg tgggtcaggc gtcggcgacg gccccggact
    23641 gcgcccgctg ggcggccacc tgcccgtgaa cctcggccat gtccagaccc ctgacgccct
    23701 cgatgacctg cgcgagggct ggcgcgggca gcgccccagg ctgagcgaac acaaggacgc
    23761 cttcgcggaa ggccatcaag gtggggatcg acgtgatgcc ggccatggcg gcgagggcct
    23821 gctcggcctc ggtgtcgacc ttggcgaaca cgacgtcggg gttctcgtcc gaggccttct
    23881 ggaacaccgg cgcgaacatc cggcagggcc cgcaccaggc ggcccagaag tcgaccagga
    23941 cgaggtcgtt ctcggtcacg gtctgctcaa acgtggcagc cgtgaggtcg atggtgctca
    24001 tgaggctcct tgtgcgaatc ggtggaacgt cagccggatc actgggtgac ggctcctccg
    24061 gccgcggtcc acgcttggat gccgccgccg aggtggaagg tgttggtgaa gcccaggccc
    24121 ttcatgatgg ccaacgcggt tgcggaccga ttcccgctgc ggcagtagac ggcatacggg
    24181 acgtccttgg ccaggccacc cacgatggtg cggaagtccg cggcgttgac atcgacgttg
    24241 acggcaccgg cgaggtgtcc ggcggcgaac tcggccgggg tacggacgtc caggacgacc
    24301 gtaccggggc gtttgagagc ggcagcgaag ccggcagcat ccacctcggc cccgttggcg
    24361 ggcgccgcag ttgccgccgc ggcggatgcc aggggggccg agcccgagcc tgcggagccg
    24421 gaacacgcac cgacggtgat ggccgcgacg gcgacggtgg cgacgatgac ggccaacctg
    24481 cggaaggtca taacaagggg ctcctgggac tcgcgcgaca cggctcgctg gggaagaaca
    24541 accaacctga ctagtatagc gcataccccc aggggtatat cccagagggt aggtgacagg
    24601 ccccaaacat cggccagggt ggcccggcga gcgccggaac caccctggcc caaggagagc
    24661 tctgcggtta cggcatgtcc acccgaacga tggtggggtt ggttgccgac gcctcgcccg
    24721 tataggcgac cgaaccgagg tacttcgtgg ccgctagtag cccgttgaag ctcagcacaa
    24781 tctgtccggt ctggccgatg gtcgccgttg ccggcgcggt gaccgtcatg ttcccggcgt
    24841 cggtcgaccc gagcaaccac tggaacagcg tgtagttcgc atccggaccg tcggtgcccc
    24901 atccatggac gtagaccgtg taggtgcccg gcgcggggtt gacgaggctg acctcctcgg
    24961 ctgaggtgcc acttccgctg cctccgactc gcttactacc cagatagaca tagagatcca
    25021 ggtcgtcgac ggcaccgtcg acaaagtcgt cgaacagcga gaaccgggcg taggtcgtgc
    25081 ctgccgggat gaccacctcg tgtgccgtga cgcccagtcc ggtcggcgag aacgagtcgt
    25141 tcgggtcgtc gagcacgtga cctggggtct tcacggccgg gatgaggccg cgcggggtgg
    25201 ccgtgaacgg accggtgtag ccgaagccca cctgataggt gatcgctgcc ccggagccgt
    25261 tgacctgcgt cggcgcagcc agggccaccg ggcggatgac cgcgggaatg cggacgttgt
    25321 gagtcccgtc cgaccacgtg agctgcccac cggcatacgt attgagttca gccgtggagc
    25381 gcatgaaggt gagcttcacc gtctgcgacc ggcccggagc aaccgtgaag gtctgcgggt
    25441 cagcagtcac cgtgatgccc gcgagaccgg acgtcgtcag cgaataggtc gcccgctctg
    25501 tgccgacgtt ggtcaccgtc cgcgaaaccg tctgcgtgcc ggccaacgac ccgatggcga
    25561 tcgaggccga gttgaagtca ctcgggtcgg tcaggaatcc gcccgcggcc agcttcgcgc
    25621 aggtgctggg cccgactgcc gaggtgctgc cgcagaggaa ggcgagccac tggttccagc
    25681 cgctgtcgaa gaccaagccc gggtcggtcg ccttattggg cgtgacatgg ccggcgccct
    25741 ggttgaagat gaccgtggcg ctcgtgttcg gtccgtcaag aacgtcaccc gcggtcgtca
    25801 tgaacgccga cttgatcgcc atgggcgacc acgagggctt ggcctgcttg agcaacgccg
    25861 ccaggccggc catgtgcggg gccgacatgg aggtgccgct gtagaggtca aagtcccgtc
    25921 cggcgttccc cggcggggcg acagcagcga ggacgtcctg ccccggagca ctgatgtccg
    25981 gcttgagcag gtcaccggca gcggcgatga gcgggccacg ggaggagaac accgcggtga
    26041 acggggcagg cgcgtcatag atgacggtcg actggttgat cgttgcggtc gctccggcgg
    26101 tcgcggcata cgcgttgatc gcctcgcccg ccgcctggtt gacgtgcacc gacggcacga
    26161 agtggaagtc agcgttgagc gagttgtcgg ggctgttgaa gaggatcatc cccaccccgc
    26221 ctgcctcctt cactgcgagg gacttgttcg ccctcgccgt ctcgccacgt cggcagacga
    26281 cgatcttgcc ggccaccttg gccggatcga gggtgacctc gccgtcggta gccccgacac
    26341 accaagccag gttctccggg tcggcgccgg tgagccctgc ggcgctcgcg tcgatgagcg
    26401 gcgctggtcc gaccggagtc gcgaacgagg caccttcgta agtggccccg ttgcccaggg
    26461 tcaccgaacc cacgccgttg cgattgtgcg tggcagcagc aaccgtcgtc gtccacggcg
    26521 ccgggtgcgc aacggtggac tcctcgggac cgctgttgcc cgccgaggct gcgacgaaga
    26581 tgcccgcgtc tgcggcgtag aggaaggcga tctccaccgg gtcggcgaag ttcgtctgcg
    26641 agccgctcac cgagtagttg atgacgtcga cgccgtcggc gacggctcgg tcgatcgcgg
    26701 ccacgaggtc cgagttgtag ccggaaccgc cactgggggt ctcccagagc gccttgtatg
    26761 ccgcaatccg cgcccgtggc gcgaggccgc tcacagcgcc gatgcgcgcc gcgtcctggg
    26821 tggtcggcac cttcgcgttg ccacccgcgg tgctggcggt gtgggtgccg tgcccgccga
    26881 agtcgcgcgg tgaggcgaat tcctcctctg caactccgcc gttcccgccc caaccggcgt
    26941 tgaagtactg ggcaccgatg agcttctggt tgcacatcga cgcgttgaaa ccctcgccag
    27001 gggtgcactt gccgtgccac ccggggatct gctggtaggc caggaaaccc tgcgctgccg
    27061 ggttgccatt ggcatcaacg cggtcggtga agctcagcga ctcgggccag atgccggagt
    27121 cgatgatgcc gatgatgacg ccctcgccct tgaggttgcc gccggccggc gtccagagcc
    27181 cgccggtggc tgcgtcgttc aggccgagga aagacggcgt cgaggtcgtg tcacccttac
    27241 gcagttcctg gggcgtgacg gccacgacgc cgggagcctt ggccaaggcg cccgcctgag
    27301 ccccagtgag cttggcggcg aagccgttga acgagaacag gtagtcatag agctttgccg
    27361 ccccacccac cttgttcaaa gcgctgtcgt gggacccggt gaggtaggac gcgtacttcg
    27421 tcgcggccgc tgtcgtggag tcgaacttct tgcccgcctt ggccttcgtc gccgtgtatc
    27481 cggcgaggct cccgtcatag gaggcaaccg gaaggtcgcg catctggacg atgtaggtcc
    27541 catcggagta tgccgccccc gacgccgcag gagccgaggc cccgatagcg gcagcattcg
    27601 gggcagcccc tgcactgagc ggggtagcac cggccgaccc gcttcccatc agcgtcgctc
    27661 cgaccagaat gggaatggcg accagcgcgc cgaccgacgt gcgattcatc agtgctcctg
    27721 acgtgtgaga gacccgcagg gccatggcag gaccgacccc cgagacgggg accttgatgg
    27781 cgaggctaac ggccgtcggc ccatggggcg gggtcaatca ggtaaagaaa tcttcagcac
    27841 gacgacgggc acctcagcgc aggagcatca gcgcgtgcgc ggaggcgtct cggcgggccc
    27901 ttagagcggg tcccctgtcg ggcccgcgcc gacgctcgcc gggacttccc agatcaccct
    27961 ggtcccaccc aggaccgcca tcggcgacag caccacgagc gacccgcggc gcccggcggc
    28021 gcgctcggag aggttggcga ggccgctgcg ccggcccgtc tcgatcatcc cgacgccatc
    28081 gtcctcgacc gtgacctgca cgaggtcggc ctgcacgacg acagcgacgt cgacctcgcc
    28141 ggcctgggcg tgccgagcca cgttcgacag ggcctcacgg atcacggcga gcaggtccag
    28201 gcggagccac tcgtcgaccg tctcggggtc gtccaacgga ccgacgacgg tgaggcgggg
    28261 ggtgaagccc aacggttcgg cgaagctcac gaccaggtcg tgcagttctt cggacaacgg
    28321 ctgggactcg ggctggtgca gggcgaagat catccgtcgg atgtctttga tcgccaggtc
    28381 gagttcgtcc accgcctcgc tcagacgctt ttggacatac gggtgggcag ccacccgcga
    28441 cgccgactgc aggcccagac ctgtggcgaa caaccgctgg atgaccacgt cgtgcatgtc
    28501 ccgcgcgatg cgttcgcgat cctccaggac caccatgcgg gcccggtccc gctgggcgct
    28561 ggccgcggtc agcgtgaccg ccgcgaggtc ggcatactgc ttcaacggct cggccgcgtc
    28621 gtatgccgtg tgcgactccc cttcgcccca cgcgacgacg agcagaccca ggctgcccgt
    28681 gcccacgctc atcgggacga tggccgtctg gccgtgcggg ggcagcccgc tggcttcgcg
    28741 caactgggcg gccagcgagt cggcgaccgc ctcaccttcg cgcgacaaca gcaacagcgg
    28801 ctcgccagag tcgaggatcg tcacccagtg cggttcggcg agaggggtgc cgacgatcaa
    28861 gcgcgaccgg gggtcggtgc cggtcgacgc ctcaatgatc aactggccgt cctcccccgc
    28921 gagcgcgacc gccgccaact cggcttcggc ggccaattgg gcacgctgga cgagcatcga
    28981 gagggccacg cgttggtcgc ggccgtaaag cagggcctgg gtcatctccc gcagcgcgtc
    29041 cgaccactgt cgccccgacc gcgccatgtg gaacatccgg gcgttatcga tcgccatccc
    29101 ggctgcagca gccagcgcga cgatgagttc ctcgtcctcg ccggtgaact ccagaccacc
    29161 caccttgtct gcgaggtaga tattgccgaa cacctcgccg cgcacatgga tgggggcccc
    29221 gaggaaggtc gtcatcaccg ggtgtcccgg cgggaagccg acagcgtcgg ggtgttcgcc
    29281 gaccgcggac agccgcaacg gtcgggggtc gcggatgagc gtgcccagga caccgtgtcc
    29341 gaacggctgc gaagcgacct gaccggcctc ctcaggcgtc aaccccgacg tgacaaactc
    29401 catgagccct tcaccgtcgg ggcgcagcac acccagggcg ccgtaccggg cccccacgag
    29461 gtcgcaagcg ctacgcacga tccgagtgag cacctctgag agatccagac cgcttccgac
    29521 cgccagcacg gcgttgagca acgtcgtgac ggcatgtgag gagcgcggca tgctcaagcc
    29581 ggtgaggttc aactgcacgt aacgatgcta ggccagtgcg ctcaggcggc gctgatgggc
    29641 accgtgacga cggggcactc ggcgtgcgcc acgcaccgtc gggcaatggt ccgcgccgtg
    29701 ccctcgtcgg tgccgacgat cagggcggcc gcgccacggg tgagttcgag gagggtcggc
    29761 gtcgggtcgc cggtgacgac ctcgaagcga cacggtatgc cgcgcagccc ccgcagcgcg
    29821 tggacggcgg ctcggaaggt ggcctggtct gcctcggccc gctcgtccgg gctcaggccc
    29881 tcctcgacga tctggacgaa ccgcaaccgc gcaccccgcc gaactgcctc ggtgacggcg
    29941 gccttggcca ccgattctgc ccgcccgtcg ttgagcaggc cggcaacgac gtctcccgat
    30001 cgccccggcg tatacgggac gccctgctgc gccctcgttg tgctcacggg gccatcgtcc
    30061 gacgtggctc accgtcgaat cagggcccaa agtccctcag cccgtgccgg tcccggcgtg
    30121 tcggccggga gtgccccaca tgccccttgg gagaacgctt ccagtcaggg ccgacgcggc
    30181 cgactcctcg tcggatgggt cgccgcgtag acggccgcct gggtgcggct gtccatcccg
    30241 agcttgtcca ggatcgaggt gacgtagttc ttcacggtct tctcggcgag gaacatgtcc
    30301 tcggcgatct gccggttggt gaggccttcg gcgatgtgct cgaggatccg tcgctcctga
    30361 ggtgtgagcg aacgcaaccg agggtccacc gaggactccc ccaggctcga cttcacccga
    30421 gccatgacct cggggcggat gaggtgctct ccggcggcca cccgacggat agcgtcaacg
    30481 aggtcattgc ccttgatgtc cttgaggacg tatcccgagg cgccggcgag cacggcggcc
    30541 ttgagggcct ggtcgtcgtc gtacgacgtg agaatgagcg cccgaagcgt cgggtcgacg
    30601 gcccggaccg cgcggcatac gtcgatgccg gagccgtccg gcaggcgggc gtccaggacg
    30661 cagaccttcg gccgcaaggc gggaatgcgt cgttcggcct ccaacgccga cgcggactcg
    30721 cccacgacct cgatgtcccc tgagagttcg agcagttcac gcaggccgcg ccggacgatc
    30781 tcgtgatcgt cgagcaggaa aacagtgatc gtcacggggc ctagggaatc acacttcccg
    30841 ggtggtctgg ggggtcactg cgattcgtaa aaaagcgact cggtcacggc acgcgcacgg
    30901 cgggacgcac gaaggtagtc ctccatcagc ccggcgcccg tccctgggtc gcggccaacg
    30961 atccgcccca ccccatcggc gtcgcggatg tcggtgggaa cggcatcccc ggggcgcccg
    31021 cgccacaaca ggtttgcgtt gcgcaaccgg ctcgcgttct cgtatgccgc cgccaacgtc
    31081 gcgctgtctt cctccccgac cagcccggct gcggtagcgg ctcgcaggcc cgccatcgtc
    31141 ccgggggttt gcaggctggg gacctcatgg gcatggcgca gttggaggag ttggacggtc
    31201 cattcgacgt cggtgaggcc gccccggccg agcttgagat gggtgcgtgg gtcggcgccg
    31261 cgcggcagcc gttcggactc cacccgtgcc ttcatcgtgc ggatctcgcg cacctgggtc
    31321 gcgtccagcc cggccgcggg atacctcagg gggtcgatca gcgcggcgaa ggcctcccac
    31381 aggccccgat ccccggcgat cggccgggcc cgcagcaacg cctggaactc ccaggtgagg
    31441 gcccaccggt cgtagtagac ggcatacgag gcgaggctgc gggtcagggg cccgttcttg
    31501 ccctccggac gcagatcggt gtcgaggacg agcgcatccg acccggtcgc cgccacgagc
    31561 cgcacgagtt cttggacgat ggccaaaccc tgccgatggg cggcctcatc ggacaccccg
    31621 ggccggggct ggtggatgac catgacatcg gcgtcggacg cgtagcccaa ctcgcgcccg
    31681 ccgaggcttc ccatgccgac gacgagcacg tcgacgagca acccgtcctc gcccccaccg
    31741 acgacccggg tcgcgacctc cagggcggcc tcgacggtcg ctccggcaag gtcggccagg
    31801 gctgccccga cctggtccgc ggcgaattga ccggtgagat cgccgaagac gatccgcagc
    31861 agttcgtgcc gccgcaatgc cccgaccgcc gccatcgcct tgtcggggtt gtctttacgg
    31921 gcagccgcgc tgcgcaacgt gatcgccagg gactcctgac tgcgtggccg cagcccgtcg
    31981 gggtcgccga ggatcgacac cgactcggga gcgcggatga ggaggtcggc ggcatacctg
    32041 ctgcgtccga ggacgtgcgc gagccgctcg gcggcactgc cttcgtcgcg gagcatcttg
    32101 aggtaccagt ggattgagcc gaggtcgtcg ctcacccggc ggaaggcgag caacccggcg
    32161 tcggggtcgg cctcgtcggc gaaccacgag agcatcaccg gcaggagggt gcgctggatc
    32221 gccgcacgcc gcgagacccc cgcggtcaag gtctccaggt ggcgcatggc gccggcgggg
    32281 tcgcggaagc cgagggcggc gaggcgctcg cgagcctgct ggggcgacag gctcgtgtcg
    32341 tcgctgctca accgcgcggc ggcggccagg agcggccggt agaagagccg ttcgtggatg
    32401 cggcgcacct cgcgtgcctg cgactgccac atcgcgacga cggcctcggc tggcttgctg
    32461 gtcagaccca acgcgcggcc gaggacgcgc aactcgtcgg gagcggtcgg catgaggtgg
    32521 gtccggcgca gccggtagag ctggatccgg tgttccaagg tccgcagcag ccggtatgcc
    32581 gcgtcgaggg tcgccgcgtc ttcgcgcccg acgtagcccc cagaggccag ggcgtgcagg
    32641 gcctgcaaag tcgtcgccga acgcaggctc tcgtcgtgtc gaccgtggac gagttggagc
    32701 agttgcaccg agaactcgac gtcgcgcagc ccgcccgggc cgagcttgag ttggcgggcg
    32761 gcctcggcgg ccggcacgtg ttgctcgacc cgacggcgca tcgcctggac gtcctcgacg
    32821 aagtgctcgc gccccgcggc gccccagatc atcgatcgca gggcgtcctt gtaggcgatg
    32881 ccgacctcac ggtcaccggc cgagacccag gccttgagca atgcctggaa ctcccaggtc
    32941 ttggcccagc gctcgtagta ggccttgtgg ctggcgacgg tccgcacgag cgggccggcc
    33001 ttcccctccg ggcgcagggc ggcatcgacc ggccacagcg tgccctcgcc ggtctgggcc
    33061 gcacaggcct tcatcgtcgc cgtcgcgagc gccgtcccga ccttgatcgc gcgttcctca
    33121 tcgaccccgt cggccggctc gacgacgaag atgacgtcaa cgtccgagac gtagttgagc
    33181 tccccgccgc cggtcttgcc catcccgatg atggccagcc ggcagtcctc gtgaccgggc
    33241 ccgaactcct cgcgggcgat gtcgagggcg gcctccagcg cggcctcggc cagttgggcg
    33301 agcgcctcgg cggccgctgg caggtgcgaa accgggtcgg gatcggacag atccaaggcc
    33361 gcgatcccca cgagttcacg gcgataggcc acccgcaacg cgtcgtatgc cgttgcggca
    33421 cctcgcgcct gcggcgtcac ggccgacgtc agcgcggtcc gcaggcccgg tccggtcggg
    33481 ggggtcgcgt cggtcgcagc gacccaatgc tccgggtggg cagcgaggtg atccaccagt
    33541 gcccgcgacc caccgagggt cagcagcagc cgtcggcgcc ggacggagtc ggtgctgaga
    33601 acctcagcga gagcgcgtgg gtcgctgtgg tgcgcgggcg gcataccgag gacggcctcg
    33661 accagccgca ccagcccgag cgcggcgagg tcggggtcgg ctgctgaggc cagcccttca
    33721 gcgacggagg tgcggacagc gcgggtgctc tcgatgagcg cgtcggtgtc gagcgtcgcg
    33781 ccggcgcccg ggtcaccccc gggagcgaac aggcgcaaca ggacggcgtc acgcagcagc
    33841 cccgccgacc gctcgggctc ggcgaacccg agccgagcca tcagggcgac agcgctggag
    33901 gaccgatcga ccacgacgcg ctccccttct tgacggtcct agagccgcgt gaggtagcgg
    33961 tccaactcga actgggtgat ctgctgacgg tactcccgcc attcggcccg cttgtt
//
LOCUS       DAOMZY010000003        33964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_958_length_33964_cov_5.987290, whole genome shotgun sequence.
ACCESSION   DAOMZY010000003 DAOMZY010000000
VERSION     DAOMZY010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 33964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33964
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_958_length_33964_cov_5.987290"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PLK64_00335"
     CDS             <1..530
                     /locus_tag="PLK64_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HPV78215.1"
                     /translation="GWFERDYVDYGFDFGTPGSRLDALARDLPVLKDRWTRLNPLPTR
                     AIPVLVGGGGERKTLRITAEHADIWHGFGDLATIRHKHLVLDEWCDRVGRDPLAIERS
                     AGVAYTPARLPEDATLDFAGQAQALFEVGTRLFTISLTTPPYDLGPIREMLAWRDAVN
                     ASRAADQGSTTSLPV"
     gene            532..1104
                     /locus_tag="PLK64_00340"
     CDS             532..1104
                     /locus_tag="PLK64_00340"
                     /inference="COORDINATES: protein motif:HMM:NF022167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HPV78216.1"
                     /translation="MAVSDIEAVEILDLLETVGAQPWVAGGWGVDALMERQTRMHRDL
                     DVMVLAKRLDDALAALRANGFQVTVDWLPVRVELDDGLRVVDVHPVHPDGSGGWWQAA
                     PDDGRYLYPAGSIVGGLIGGHTVRCASPALQRQVHTGYPLRLVDEHDIALLDEMLADA
                     DADADDLEAAGNAAQGPGPWAAGRGIPETL"
     gene            1115..2353
                     /gene="serB"
                     /locus_tag="PLK64_00345"
     CDS             1115..2353
                     /gene="serB"
                     /locus_tag="PLK64_00345"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HPV78217.1"
                     /translation="MTGASPHTLRVTLSGDDRPGVTFALLAAVAEAQAEVLDLEQMVV
                     RDHLTLSVLLGGADTDRLTSLAHSVGHRLGMRTRTRVGQGDSNTRRDGRAHVVALGQP
                     LTAGGLAAVTARIAAHGANIDRIRRLSRYPVTTIELDVSGADVPRLRVELATVAAAER
                     IDIAVAPAGLARIGSRLVVMDVDSTFIQQEVIELIAAHAGCEEQVAAVTERAMRGELD
                     FAESLRARVALLAGLPATVLDEVRDEIVLTPGARTLVRTLKRLGDTVALVSGGFLEIV
                     GPVAADLGIDHVLANRLEVADGRLTGRVDGEIVDRPGKAAALRRFAAEAGLPLSRTVA
                     VGDGANDLDMLAAAGLGIAFNAKPLVREQADASVNVPYLDAVLFLLGITREDVEDADA
                     DAGTPTPAPPVISPGNPTRV"
     gene            2356..3300
                     /locus_tag="PLK64_00350"
     CDS             2356..3300
                     /locus_tag="PLK64_00350"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HPV78218.1"
                     /translation="MVSNAGPADEAGMPPTSPPVRRTLLIVGGAEDKLGRSVVLRRFV
                     RLAGGRASRIVVIPTASSFSTEASALYRSVFARLKAGQDFEIVHPPTRAAASSAELVA
                     RLDAATGIFMTGGSQLKLSQNIVGTPVGDAIHRAYQRGAVIAGTSAGASIMSQFMISL
                     GDEGVTPRQRSSQLSAGLGLVTGVVVDQHFDQRGRYGRLLSIVANSPNLIGIGIDENT
                     AAEIRDERLMSVVGAGAVFVLDARHAITDAPEARRGAPLLVSGAVVHTLPAGATFDLE
                     SVGLVRFVERHPDIEVAVVAAKDRSPMVPAHAAAPQPR"
     gene            3344..6211
                     /gene="cphA"
                     /locus_tag="PLK64_00355"
     CDS             3344..6211
                     /gene="cphA"
                     /locus_tag="PLK64_00355"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HPV78219.1"
                     /translation="MAKDHPTPTGPIAPDLRILETRVYRGPNVWSYAPAIHLVVDLGV
                     LEGYPSDSIDGFTDALVALLPGLQRHTCSVGHAGGFIERLEDGTWLGHVAEHVALELQ
                     STAGHDLRRGKTRMVKGRPGVYNVIFGYVDETVGIAAGELAVRLINHLVQPTDGFDFT
                     EQLDAFLRRADRVAFGPSTAAIIEEAVSRDIPWLRLNSASLVQLGQGVHAQRIRATMT
                     SRTGALAVDIASDKDLTGKLLSAAGLPTPKQQAVRTRRGVLDAARQIGFPVVVKPLDG
                     NHGRGVCLDLQTLDEVDKAYDIAQEQSRSGVLIVESFVTGRDYRCLIVGGRMQAIAER
                     VPAHVVGDGEHTVKELVDITNSDPRRGVGHEKVLTRIKVDAAAIEVLEGQGYLLTDVP
                     DAGVMVKLALTGNMSTGGISVDRTFDAHPDNVEIAEEAARMVGLDVAGIDFICPDIAA
                     PVRETGGAIVEVNAAPGFRMHTHPTVGVPQFIAKPVVDLLFPPGAPSRVPIVAVTGTN
                     GKTTTSRMIAHIFKGLGRKVGMTSTDGVVIDERLVIKADASGPKSARMVLMNPRVDFA
                     VMEVARGGILREGLGYDRNDVAVVTNVAPDHLGLRGIDTLAQLAAVKAVVVEAVPKNG
                     FAVLNADDPHVRSMRRKCSGEIVWFTLAAPGSDIREFVDAHCRRGGRALYLDPTDRGD
                     MIAIQHGRRAMQLAWTHLLPATFGGTARFNVANALAAAGAAFAAGAPLHDIRQGLRTF
                     TSNYYLSPGRLNLVEVNNVEVFVDYCHNAAGMRALGDFVDRYAATRAGRADLGRVSRI
                     GAIGAAGDRRDSDLRELGAVAAEHFDVLVIREDARLRGRRPGEAAQLIREGVKEAMQK
                     GVRCRQVDIVLDELQAVGDVMARANPGDLVVVCVDNSAEVFAALEDMTRQAQPGAHPG
                     GVSDPDFDPVVLTGAAAVSGAAAEGRALSTPAPETTGAPNA"
     assembly_gap    6276..6375
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6539..7981
                     /locus_tag="PLK64_00360"
     CDS             6539..7981
                     /locus_tag="PLK64_00360"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPV78220.1"
                     /translation="MIAGLLAALVAPLSLRGGPATSGDLDLAARVRAAVEDDRGYQAL
                     HVSEITQTSVRHTGVGSIDPGGAEALGAATPIELGSITKTFNGLLLADAITRGEVAVT
                     DPVSTYLPRLAGSPIGGVTLEELASHRGAVPPFPPPTMARGLWGLLTNTDQLSGDQLD
                     WVLDQAAAMPLFGGRGTVQYSNLGATLLGWALASAAEQPDWQTYVTTRLLTPLAMTHT
                     RFAATRDQIPPDAPRGRLVGGRQAVQGVSPVYQPAGSSTWTTPEDIARYAQAILRGTA
                     PGLPALDPRFAGEQAASPGDPRVGYHWFTLTVAGRTVQWHNGATAGYTSMLVIDRLAG
                     RAVFVVGNSTRPVDPVAIRLLTGVADGPGSTDVPMPVIALAATTVALILIAGAGFAAY
                     RARTRLQLLRATAGALLGLSLWRVAGPWDHAPGLLWILVAAATAATVMLGIARVRTRP
                     WHRPRLAALSWAGAVLALAAAGLVLSMGLH"
     gene            complement(8037..10244)
                     /locus_tag="PLK64_00365"
     CDS             complement(8037..10244)
                     /locus_tag="PLK64_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPV78221.1"
                     /translation="MEPVHADEVRILEGPNLYVTRPAVKVSLALPGVAQLDVHAALDL
                     ARRVGLRTARPGRPDTGQRQRFLMRLVEHLVRRVATESGARRLAVRTRAGRHTEDVVV
                     AVPWRHRGRAIALGECLAPLLSDLLNADADTEALIAAAGRAVAEAPDGEPAAVLTPTV
                     PVIAITGTNGKTTTTRLVAHMVMTAGLRTAWSSTDGVVVMGEMVQPGDYSGPGGAREV
                     LSTPGLQLGILETARGGMLLRGMGVNQTDVTVVTNVAADHLGLNGIDTLDQLAEVKAI
                     PTLIVKPDGWTVLNGDDPRVWAMRHRAKGKIWAFSLDPNAPALREAVAGGGRGITVLD
                     GEIVVLTPGRDPDRLVKVVDVPMTLAGLSSINIANALGGTAAGLGLGLTRAAVVEGLR
                     TFRPDPVLNSGRFNTYSVPLTGGGSATVVIDMAHNEAGLEALLEVCRGLTAPGGRVRL
                     GIGGTGDRLPEANAALGEIAGRGADHVVLAHKLKYLRGMSLEQMDSLLRAGLARSGMA
                     EVAGFLTEIEALTTLLAAAESGDVIGLMCHVQRAEVADLIAQRGGTVDDPRTIRSKVI
                     AARGMHEAEDDIAAIWALTDAGERVTAAGRLATAHPSDPRLAYEWACAIDATADRARA
                     VSLYHRALDNGLREPHRHRAQLQAAAAYRDLGHPEIALELLEAVAATHPGSSVAAALR
                     ALVLVDTGDAAEAVADLVDALMEHATDEDAAAYRAVLHHAAAQLRTDRPGVPD"
     gene            complement(10299..11054)
                     /gene="fabI"
                     /locus_tag="PLK64_00370"
     CDS             complement(10299..11054)
                     /gene="fabI"
                     /locus_tag="PLK64_00370"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HPV78222.1"
                     /translation="MFDGKTLLITGVLMDTSIAFHVAKLAQEQGASVILTSFGRTMKI
                     TQTIARRLPVTPPVVELDVSNPEHLDTLAERLREHTDRLDGVLHSIGFAPLGAFTFLD
                     ATWEDVGTAVHVSAYSLKALAVAALPLMEPGGSIVGLTFDARYAWPVYDWMGVAKAAF
                     ESTNRYCARDLGPKGIRCNLVAAGPIRTTAAKSIPGFEVFEEQWDTRAPLGWDVTDPL
                     PTAKACVALLSDWFPATTGEIVHVDGGVHAMGC"
     gene            complement(11147..11869)
                     /locus_tag="PLK64_00375"
     CDS             complement(11147..11869)
                     /locus_tag="PLK64_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HPV78223.1"
                     /translation="MTDAAPGRTVLVTGGNRGIGLAIARSFAEAGDRVVVTTRSGEAP
                     EGLDAVPCEVTDSASVDAAFAAAEGIFGSPVEVLVANAGVTRDQLLLRMSDEDFDSVI
                     DTNLAGTFRCARRASKGMIRLKRGRMIFISSVVGLYGSPGQTNYAASKAGLVGMARSI
                     SRELGGRNITANVVAPGFIDTDMTAALPDERRAAYLASIPAGRFAAPEEVAAVVRFLA
                     SPEAGYVTGAVIPVDGGLGMGH"
     gene            12073..12378
                     /locus_tag="PLK64_00380"
     CDS             12073..12378
                     /locus_tag="PLK64_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3099 domain-containing protein"
                     /protein_id="HPV78224.1"
                     /translation="MTSAPMSLAQDTDMRMRRYLITMAIRVLCFVLAVVFEGWLRWVF
                     VAGAAILPYIAVVLANAVGPRWGARISGIDKYEPTHPELSASTPTGEPVLGADPFRR"
     gene            complement(12395..13426)
                     /gene="moaA"
                     /locus_tag="PLK64_00385"
     CDS             complement(12395..13426)
                     /gene="moaA"
                     /locus_tag="PLK64_00385"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HPV78225.1"
                     /translation="MTRLPDPRTRPPEGSALIDTYGRIAQDLRVSLTDRCSFRCTYCM
                     PAEGLPWIPQPQMLSDDELMRLIGVFIGAGVTQVRLTGGEPLLRRSIVELVAGIAALR
                     PRPAIAMTTNGAALAAVAAPLAAAGLDRVNVSLDTIDASQFLALTRRDRLADVIAGLA
                     AAADAGLTPVKVNAVAMRGVNDAVVADLLAWCLARGYELRFIEQMPLDAQHGWDRSVM
                     VTAEEIKAGLEARFELTPVESTDRGSAPAARFLVDGGPATVGIIASVSEPFCSACDRV
                     RLTADGQVRNCLFSERETDLRGPLRDGASDAELLGLIRGEMWRKGPAHGIGRPDFHQP
                     DRPMSAIGG"
     gene            13459..14259
                     /locus_tag="PLK64_00390"
     CDS             13459..14259
                     /locus_tag="PLK64_00390"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HPV78226.1"
                     /translation="MWRAIFTRRILYAVAFALGFGTVCFLLGQWQLSRYEEKDARATA
                     VEQNYNRPAVPFAGVLPSVTTPLAESQVWTQVTLTGRYAASTHLIVRGRALNTAQGME
                     ILVPFQVDGGAVLLVDRGWVPNGDQAADLPTVSAPPAGTVTLTGWLKPGEEDRGKDLP
                     AGQLATINLAQAQEQVPGQLYAAYLVLGSESLGEGLPAPARPTPLEAPTPDRGPHFAY
                     ALQWWLTTLLGFGFVVYLYRHPPGGVPNATPTEAPEPKKVRIWDEEDG"
     gene            complement(14280..16172)
                     /locus_tag="PLK64_00395"
     CDS             complement(14280..16172)
                     /locus_tag="PLK64_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV78227.1"
                     /translation="MSFGQGGPMGMMRGMAKDQSVLRARLEQGTTRRVLGYARPFVRS
                     IVAFLTIVILDAGIVVAVPLLLRDLVDLGVIPKAPDVVVRLALLVAALAVADAILSIA
                     QRWFSSRIGEGLILRLRTEVFAHVLRQPIAFFTRAQTGSLVSRLNSDVIGAQQAFTSV
                     LSSVVSNAVSLVLIVAAMATLSWQLTLAALALVPFFLIPARFMGRRLSELTRTAMTAN
                     ADLGTRMTERFNVAGALLVKLFGTPEREEREYAVRAKLVRDTGVKIAMNRTVFMVAMS
                     LVAALATAMVYGVGGLLAVNGALTVGTLLALAALLARLYGPLTALSNVRVDVMTALVS
                     FDRVFEVLDLKPLVAESAHPVTLPAGPVAVEFDGVGFAYPSADEVSLRSLESTASGDR
                     AGGGPVLDEVSFTAYPGQLVALVGPSGGGKSTITALVARLYDPSHGTVRIGGVDLREA
                     AFGSLHETVGMVTQEAHLFHDTIRENLRYAAPDATEEQMRAALEAAQVLHLVDSLPSG
                     LDTVVGDRGHRLSGGEKQRLAIARLLLKAPHVVVLDEATAHLDSESEVAVQRALDVAL
                     AGRTALVIAHRLSTVRGADQILVVEDGRIVERGKHAELLGRGGLYADLYRTQYFEDST
                     TKAGPW"
     gene            complement(16315..17106)
                     /locus_tag="PLK64_00400"
     CDS             complement(16315..17106)
                     /locus_tag="PLK64_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPV78228.1"
                     /translation="MTSLPQPHPAIRVEQDGPVVTVTLCNPEHLNAQVPSMWLALADI
                     AHGLAGEVRVVVLAAEGRSFSAGLNRGMLRPGGMPGEPDLLAAAASSPEAMTELIAPF
                     QEGFAAWRRVAPVVIAAVQGHAIGAGFQLALAADLRVVTDDVSFAMREVSLGMIPDLA
                     GTRPLVEAVGYSRALELCATGRFVGAEEAVRMGLATLAVPPTELSATTSDLVQALLGT
                     PPAALWALKPLLNHALTATIDEQVVRERVAQGHLLHGMARAAGLS"
     gene            complement(17175..18371)
                     /locus_tag="PLK64_00405"
     CDS             complement(17175..18371)
                     /locus_tag="PLK64_00405"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPV78229.1"
                     /translation="MVTSLPRYAARSAARRTSGAHPRGPRARRALVTLTVLTVTAATG
                     CTAGQGDGWSALGPLSAQVGAVVTLTAVGDLLVAGRSTAAGEPAVEVLADGTWTSRPV
                     QAVTPYGRVAQWLSIAAGPDGSLVAVGGARGGAHANVRWTVWRGSVAAGLTEQEQTFS
                     TFGGWGAGDQVGPVLTAAGPLLVGSWEGAQAGLDLATWVPDGEAWVRQPSAGTALEST
                     PTQLMGARSVAAWGSGALITGSVVRLGDGAVRKVPAIWRSTTGNAGWTRVDLPDSGQA
                     GEAVSAACTEAECTVAGWVDGRLAVWRVGADGGARRVDGIPSVAVRDSDLIPAPVFVE
                     GRAVLLVTDHDSSRNATVVVEGRTGWSRGPGPWGTVQAFTAIGSTLYAVCQHGDTPLQ
                     VWAHRL"
     gene            18475..19857
                     /locus_tag="PLK64_00410"
     CDS             18475..19857
                     /locus_tag="PLK64_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HPV78230.1"
                     /translation="MSTVKRHKVILDEREMPTRWYNILHDLPTPPPPYLHPGTGQPVG
                     PEDLLALFPMDLIQQEMTTAQYVDIPPEVLDVYRLWRPSPLYRAHRLEAALGTPARIY
                     YKYEGVSPAGSHKPNTSVPQAYYNAKAGIKKLTTETGAGQWGTSLAFACAQFGLECEI
                     WQVRASFDSKPYRRLMMETFGGTVHPSPSELTAAGRAILAAHPDSPGSLGIAISEAVE
                     IAAQSESTNYALGSVLNHVLLHQTIIGEEALRQLAKVEETPDLIVGCTGGGSNFAGLS
                     FPFLREKLAGKMSPVIRAVEPASCPSLTQGTYAYDFGDTAGMTPLMKMHTLGHDFIPD
                     PIHAGGLRYHGMSPLISHIYESGLIEAVSKKQLECFAAGVLFARTEGIVPAPEPTHAL
                     AETVAEALRCKETGEEKVILTALCGHGHLDLPAYGAYLAGEMSDHSYEEAEIRDAVAE
                     ALTHLPTIPG"
     gene            19942..20370
                     /locus_tag="PLK64_00415"
     CDS             19942..20370
                     /locus_tag="PLK64_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPV78231.1"
                     /translation="MRGRTPGGPGEDRKTRRDWWGVVLDAPDAKALGQFYAAVFGWVL
                     NAEDPSFVTLDPGEGVGYLAVQTSQGYVPPTWPNEPGRQQMQLHLDIEVDNLDEAVGA
                     AVALGATQAAYQPQHDVRVLLDPAGHPFCLYCPGWPVPRA"
     gene            complement(20389..21099)
                     /locus_tag="PLK64_00420"
     CDS             complement(20389..21099)
                     /locus_tag="PLK64_00420"
                     /inference="COORDINATES: protein motif:HMM:NF014016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HPV78232.1"
                     /translation="MSEREPIILLAHGSPDPRHATGIAALATRVAAAGEAAVHVAYLD
                     HHGPTPADAAQAAGRGVVVPALLSLGYHARVDVPAAIEAMQAAAPGPFRAASGLGLGR
                     AVLDGVGELLVRAEVADVRDLHVVLALAGTSAPEAIEELSAALTVARAAVDGPTWAAL
                     ASVTEPTHLVGYAGPAADGVPTVVVPVALCEGVLRDRVITAALGLGLDTVAGVLADTD
                     ALARLVVARATQALLGTS"
     gene            complement(21096..22331)
                     /gene="cobA"
                     /locus_tag="PLK64_00425"
     CDS             complement(21096..22331)
                     /gene="cobA"
                     /locus_tag="PLK64_00425"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HPV78233.1"
                     /translation="MGYPLTLQLRDRRVLVVGGGRVAATRVCRLLADGAHVTVVAPEV
                     GEVVAALVAEGRVTWLARGYQSTDLMQPQRAWLVHTATGDHTVDSEVADDCEGLGIWC
                     VRADDGARSAAWSPAVANGSAGTPAEGLQIAVTGDRDPRRARAVRDAILAGLESGVLP
                     VRRVRRVPGTMKPGRVYLVGGGPGDPGLLTVRGRALLAAADVVVADRLGPTDVLADLD
                     PDVLVLDVGKRPGAHPVPQEEINALLVEHASAGRTVVRLKGGDPFVLGRGGEEALHCR
                     AHGIPVEVVPGVTSAVSVPAAAGIPVTHRGITTSFVVSSAHDGGAVAAQAPLDATLVL
                     LMGVSGIGETAARLMDAGRDPSTPVAFVERGWTPTQRTTVTTLEKAAWAVEHEQVKSP
                     AVVVVGDVVRLRETLGDLR"
     gene            complement(22333..23268)
                     /locus_tag="PLK64_00430"
     CDS             complement(22333..23268)
                     /locus_tag="PLK64_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV78234.1"
                     /translation="MPADLAVPAVAPAAAAAAETPAEPGTPVDETEALEAGLDVLDTP
                     SGPGRPRARALLGRVAPPLVAAVVLLLAWQVIVSSGLKPSYALPSPAAVWASLIEQLQ
                     SNAAQRAAFGSLRRAAVGFTISIILGTLLGIVLSRFRLIRRGIGPLVTGMQSLPSVAW
                     VPAAILWFGLTDAAVYAVVLLGSVPSIANGLLAGIDHAPPSYRRVARVLGAGTLAELR
                     WVTLPAALPAYLTGLRQGWAFAWRSLMAAELIAYSPQLGPGLGQLLDTGRELSDMPLV
                     LASILLILFVGIAVESLIFAPAERRLVRSRGLAGG"
     gene            complement(23258..24109)
                     /locus_tag="PLK64_00435"
     CDS             complement(23258..24109)
                     /locus_tag="PLK64_00435"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV78235.1"
                     /translation="MSLATTLSSAAASEPAEADATTVVGRVAGDTLEGTPAVTIEGVT
                     KTFDAATGPVIEDLSLQVAQGELVAVLGASGCGKSTLLGMVAGLDRPTRGRIVTPHGR
                     PALMFQDASLFPWLTARGNVELALRLRRGGARLNRHDRRAEAERWLDLVRLGRHADKR
                     PHEMSGGMRQRVSLARALAQGADVLLMDEPFAALDAITRDVLHEELGRIRAETGLTVL
                     FVTHNVREAVRLGDRVVLLASRPGRIVREWTVPIPHPRRIESPAVGALAVEITEQLRQ
                     EIRRHAR"
     gene            complement(24112..25182)
                     /locus_tag="PLK64_00440"
     CDS             complement(24112..25182)
                     /locus_tag="PLK64_00440"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPV78236.1"
                     /translation="MKLLALLGVVGVLAVSGCTATGSVMASGTGVGAAAGGSANTLRL
                     GYFANLTHATAVLGVKDGHFAAALGTTTLQTQVFNAGPAAVEALLAGAIDAAYLGPSP
                     AINAFSKSQGTAIRIVAGGTSGGASFVVRPGLTESTLADKVFASPQLGGTQDVALRSW
                     LSAHGYTTTVTGGGNVLVAPQSNAQTLSLFKAGAVDGAWLPEPWASRLRLEAGATTLV
                     DEATLWPQGRFVTTNLVVSTTYLQAHPEQVKALLQGAVAADAAIAADPEGSRDSVGSA
                     ITALTGAKLSTQVLHEAWSRLTITPDPIASSLQASATAAAAVGITKSPPDLSGIYDLT
                     LLNQVLTASGRPTVSAGGLGKE"
     gene            complement(25179..26465)
                     /locus_tag="PLK64_00445"
     CDS             complement(25179..26465)
                     /locus_tag="PLK64_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPV78237.1"
                     /translation="MTATIEAVPHELLRFATAGSVDDGKSTLVGRLLHDTKSVLADQL
                     AAVERVSRDRGLEATDLALLTDGLRAEREQGITIDVAYRYFSTATRSYILADTPGHVE
                     YTRNMVTGASTAELALILTDARHGILEQTRRHLAVTGLLGVRHVVLAVNKMDLVGWDP
                     SVYHRIADEFTTLAREFGISDVTAIPVSALLGDNVVDRSEHTPWYAGPTLLQHLEEVP
                     VGSDPSEQPFRLPVQLVIRPRTPAHPDYRGYAGRLTSGVVRVGDEVVIHPSGRRSRVV
                     GVDLPAVDGQVVSVAEAAAPRSVTLRLADDIDVARGDLIASPQDEPTVCRALSGPVAV
                     LSDRPLRVRDRVLARVGTRTVRGIVESIDDELDITTMTHLPTGDSLGLNAIGRVTVRL
                     AEPVALDDYRRLRHTGAFLLIDEVDGTTLAAGMAQE"
     gene            complement(26465..27439)
                     /gene="cysD"
                     /locus_tag="PLK64_00450"
     CDS             complement(26465..27439)
                     /gene="cysD"
                     /locus_tag="PLK64_00450"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HPV78238.1"
                     /translation="MTAVVGTPPAVETPAEATARPSAGRAAYRLDHLDHLEAEAIHVV
                     REVAGQFERPVILFSGGKDSVVMLHLAVKAFAPSPLPFALLHVDTGHNFPELLDYRDA
                     TVARLGVRLVVASVQDYIDDGRLRERPDGTRNPLQTVPLLDAISGNRFDAVLGGGRRD
                     EEKARAKERVFSIRDAFGAWDPRRQRPELWDLYNGRHAPGEHVRVFPLSNWTEVDVWR
                     YVERERIELPDLYYAHEREVFLRDGMWLTAGEWGGPRAGETPEVRTVRYRTVGDMSCT
                     GAVDSDALTVADVVLEVAGSRLTERGATRADDRLSEAAMEDRKKEGYF"
     gene            complement(27436..28170)
                     /locus_tag="PLK64_00455"
     CDS             complement(27436..28170)
                     /locus_tag="PLK64_00455"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HPV78239.1"
                     /translation="MSAATRSVTDLRELAHEGAALVGDGDGPAYEDRVEAARVALRWA
                     RLIFGDRLSVASSMGDEVLVHLVGQTVPGTDVFFIDTGYHFAETLGTRDAYAATGEVR
                     LRTVLPLLTIAEQAERHGDRLFERDPDACCAIRKVEPLERALGGLQAWVTGMRREDAP
                     TRTDIALVGWDARRDLVKLNPLAAWTAADVDRYTREESVMENPLRLDGYASIGCEPCT
                     RPVAEGEDPRAGRWSGRAKTECGLHQ"
     gene            complement(28167..28301)
                     /locus_tag="PLK64_00460"
     CDS             complement(28167..28301)
                     /locus_tag="PLK64_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78240.1"
                     /translation="MSGTPYFCPVCGEQDLRPDETDEQSWVCRACASVFSVTIRRSGR
                     "
     gene            complement(28298..29962)
                     /locus_tag="PLK64_00465"
     CDS             complement(28298..29962)
                     /locus_tag="PLK64_00465"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HPV78241.1"
                     /translation="MSTPLTTAARATGAWADGDRTPLNDNERFKLEDDGLNVRQRIET
                     IFSREGFGSIPVDDLRGRMRWWGLYTQRRPGIDGGQTAKLSADELDDSYFMLRIRSDG
                     GALSEVQLRTIAGISTEFARDTADISDRQNIQLHWIRVEDVPEIWRRIEAVGLNSAQA
                     CGDVPRVILGSPVAGIDVHEIIDGTPAIHEILDRYLLSPEFSNLPRKFKTAVSGSPSL
                     DVAHEANDVSFVGVVHPEHGPGFDVWVGGGLSTNPIFAQRLGAWVPIEDVPAVWAGVV
                     GIFRDYGYRRLRNRARLKFLVSDWGAERFREVLETEYLGRRLLDGPAPVEDPERRRDH
                     LGVHPQVDGRFWVGVAPIGGRVSGTTLTAVADLAARHGSGRVRLTAHQKLLVLDVPEA
                     EVATLVVELDALGLPANASEWRRGVLACTGIEFCKLALVETKARARSAVEELERRIPD
                     LDAPVTIHVNGCPNSCARFQVADIGLKGLVMTGASGETVEGFQVHLGGALGGNARLAR
                     KTRALRVEADDLVDYVEHVVTRFRTDQAPGESFAEWAHRADEEALR"
     gene            complement(30406..31668)
                     /locus_tag="PLK64_00470"
     CDS             complement(30406..31668)
                     /locus_tag="PLK64_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HPV78242.1"
                     /translation="MSAVEVWVSIEGVDVRAGTLYPHRRRGGATESASFTYVDEYIAN
                     PRAYSLEPGLPLKAGAHQSTLGHPMFGAFTDCAPDRWGRTLVKRCEAAQARAAGREPR
                     SLGEVDYLLGVRDDLRQGALRFRRGDGPFEAVGDRGVPALTDLPELLTLAARAEADSA
                     DLPDLRRLIRVGSSLGGARPKAHVRGADGTLAIAKFPSAAHDTWNVMAWEKVALELAA
                     RAGITVPASTLLNLADRSVLIVDRFDRTRDADGGATGRVGYVSAMSMLRSTDGEQGSY
                     LEMAEAIEMRSSKATTDLHQLWRRIVFSILISNTDDHLRNHGFLHAGGDAWRLSPAFD
                     VNPNPEPGPKHLSTAIDLADDTASISLALSVADYFRLGAEAANDVVVHVQAVVSQWAT
                     VARQYGLSGQEVSKMSWAFEAHSARPHP"
     gene            complement(31665..31988)
                     /locus_tag="PLK64_00475"
     CDS             complement(31665..31988)
                     /locus_tag="PLK64_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPV78243.1"
                     /translation="MTAATTGRTPVAVARALDGLGRDLTTWRKLRHLTAEQVADRAGV
                     SRSTVLRLEGGQGASVENLLRVARALGVLELLTKALDPYATDVGRLRAEEKLPTRVRA
                     RGVSR"
     gene            complement(32110..33708)
                     /locus_tag="PLK64_00480"
     CDS             complement(32110..33708)
                     /locus_tag="PLK64_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPV78244.1"
                     /translation="MIVATAVQLRVGARLLLGETSLRISEGDRIGLVGRNGAGKTTLT
                     KVLAGEALATSGSVTRTGAVGYLSQDPRTGDLEVLARDRILAARGLDVTLRELRAAET
                     AMASSDGAVQERAMRKYTRLDAEFNAQGGYAAESEAASIASSLGLPDRVLGQPLRTLS
                     GGQRRRVELSRILFSGAQTLLLDEPTNHLDADSIGWLRDYLKSYKGGLVIISHDVAML
                     DTVVNRVFHLDANRSEIDIYNVGWKPYLLQRETDERRRRRERLNAEKKASALLAQADK
                     MRAKATKATAAQNMARRAERLLAGIEGERAHDRVAKLRFPSPAPCGRTPLTAEGLSRS
                     YGSLEVFTDVDLAIDRGSRVVVLGLNGAGKTTLLRILAGVDAPDTGEVLPGHGLKLGY
                     YAQEHENLDVERTVLENMKTAAPDLGETEVRKVLGSFLFTGDDVDKPAKVLSGGEKTR
                     LSLAMLVVSAANVLLLDEPTNNLDPASREEILGALRTYEGAVVLVTHDEGAVDALEPE
                     RVLLLPDGVEDLWGADYKDLVSLA"
BASE COUNT         5108 a        11956 c        11867 g         4933 t
ORIGIN      
        1 ccggctggtt cgagcgggac tacgtggatt acggcttcga cttcgggacg cccgggtcgc
       61 ggctggatgc cctggctcgc gatctgccgg tgctcaagga tcgttggacc cggctcaatc
      121 cccttcccac ccgggcgatt ccggtgttgg tgggtggcgg cggcgaacgg aagaccctgc
      181 ggatcacggc cgagcacgcc gacatctggc acggtttcgg agacctggcg acgatccggc
      241 acaagcacct ggtgctcgac gaatggtgcg accgggtggg acgggacccg ctggccatcg
      301 aacgctccgc gggggtcgcc tacacacccg cgcggctgcc cgaggacgcg acgctcgact
      361 tcgctggcca ggcgcaggcg ctcttcgagg tcgggacccg gttgttcacc atcagcctca
      421 ctactccccc gtatgacctc ggtccgatcc gcgagatgtt ggcctggcgg gatgcggtca
      481 acgcgtcacg ggccgccgat caggggtcga cgacttcctt gccggtctga catggccgtc
      541 tctgacatcg aggccgtcga gatcctcgac ctgcttgaga ccgtcggggc gcagccctgg
      601 gtggcgggcg ggtggggcgt cgacgccctg atggagcggc agacccggat gcatcgcgac
      661 ctcgacgtca tggtgctggc caagcgactg gacgatgcgc tggctgcgtt gcgcgccaac
      721 ggtttccagg tgaccgtgga ctggttgccg gtgcgcgtcg agctcgacga cggactgcgg
      781 gtcgttgatg tgcaccccgt ccaccccgac gggtcgggcg gctggtggca ggccgcaccc
      841 gacgatgggc gctacctcta tccggccggg tcgatcgtcg gtggcctcat cggcggacac
      901 accgtgcgat gcgcgtcccc cgcgctgcag cgccaggtcc acaccggcta tccgttgcgg
      961 ctggtcgacg agcacgacat cgccctcctc gacgagatgc tggccgacgc cgacgccgac
     1021 gccgacgacc tcgaggccgc ggggaacgct gcgcaggggc cgggcccgtg ggcggccgga
     1081 cgcggcatac ccgagacgct ctagcctggg atgcgtgact ggcgcatccc cccacaccct
     1141 gcgagtgacc ctctcgggcg acgaccgacc gggggtgacc ttcgcgctgc tggcggccgt
     1201 ggcggaggcc caggccgagg tcctcgacct ggagcagatg gtcgtgcgtg accacctcac
     1261 cctctcggtg ctcctcggcg gcgccgacac cgaccgcctc accagcctgg cccactctgt
     1321 cgggcaccgg ctcgggatgc gcacccgcac ccgggtggga cagggcgact ccaacacgcg
     1381 ccgcgacggg cgcgcccacg tcgtggctct cgggcagccc ctgaccgccg gcggcctggc
     1441 agccgtcact gcgcgaatcg ccgcgcacgg ggccaatatt gaccgaatcc ggcgcttgtc
     1501 gcgctatccc gtgacgacca tcgagctcga cgtgtctggc gccgacgtcc cccgcctgcg
     1561 ggtcgagttg gccacggtcg ccgctgccga gcgcatcgat atcgccgtcg cgcccgccgg
     1621 gctggcgcgg atcggctccc gcctggttgt catggatgtc gactcgacct tcatccagca
     1681 ggaggtcatc gagctcatcg cagcccacgc tgggtgtgag gagcaggtcg ctgccgtcac
     1741 cgagcgcgcc atgcgggggg aactcgactt cgccgaatcg ctgcgggccc gggtcgcgct
     1801 cctcgccggg ctgccggcga cggtcctcga cgaggtccgg gacgagatcg tcctcacccc
     1861 cggagcgcgg accttggtgc gcaccctcaa acggctcggc gacaccgtcg ccctggtgtc
     1921 cggtggcttc ctcgagatcg tcgggccggt cgctgcagac ctaggcatcg accacgtcct
     1981 ggccaaccgg ctcgaggttg ccgacgggcg cctgaccggg cgcgtcgacg gcgagatcgt
     2041 cgaccgaccg ggcaaggcgg cggcgctgcg gcggttcgcg gccgaggccg ggctgccctt
     2101 gtcgcggacg gtggccgtcg gtgacggcgc caacgacctc gacatgctcg ctgccgccgg
     2161 cttgggaatc gccttcaacg ccaagccgct ggtccgtgag caggccgacg cgagcgtcaa
     2221 cgtcccctat ctcgacgccg tgctcttcct gctcggcatc acccgcgagg acgtcgaaga
     2281 cgccgacgcc gacgcaggca cgccgacacc cgcgcctccc gtcatctccc ctggcaaccc
     2341 cactagggtg tgacggtggt ttccaacgcg ggtcctgccg acgaggcggg tatgccgccc
     2401 acctcaccgc cggtgcgccg caccctgctc atcgtcggag gcgccgagga caagctcggg
     2461 cgctcggtgg tgttgcgccg gttcgtccga ctggcaggcg ggcgcgcgtc gcggatcgtc
     2521 gtcatcccga ccgcatcgtc cttctcgacc gaggcgtcgg cgctctatcg gagcgtcttt
     2581 gccaggctga aggctggcca ggacttcgag atcgtccacc cgccgacccg ggctgccgcc
     2641 tcgtccgcgg agttggtggc ccgactggac gcggcgaccg ggatcttcat gacgggcggg
     2701 agccaactca agctcagcca gaacatcgtc ggcacgcccg tcggcgatgc gatccaccgg
     2761 gcctaccagc ggggggcggt catcgccgga acctctgcgg gagcctcgat catgagccag
     2821 ttcatgatct ccctcggcga cgagggcgtg acgccgcgcc aacggtcgag tcaactctcg
     2881 gccgggttgg gcctggtcac cggtgtcgtg gtcgaccagc atttcgatca acgcggccgc
     2941 tacgggcggc tgctgtcgat cgtcgcgaac tcccccaatc tgatcgggat cggcatcgac
     3001 gagaacaccg ccgccgagat ccgtgacgaa cgcctgatgt cggtcgtcgg tgctggagcg
     3061 gtcttcgtcc tcgacgcgcg gcatgccatc accgacgccc ctgaggcccg acgtggcgct
     3121 cccctactgg tgtcgggggc cgtcgttcac accctgccgg ccggcgccac cttcgatctg
     3181 gagtcggtgg gactggttcg cttcgtcgag cgacaccccg acatcgaggt cgcagttgtc
     3241 gccgcgaagg accgctcgcc catggtgccc gcgcacgctg ctgcgccaca accacgctga
     3301 ccactagcgt gtgatcctcc ccactccgcg gcaaggagct cgcatggcga aggaccaccc
     3361 caccccgacc ggcccgatcg cgccggatct gcgcatcctc gagacgcgcg tctatcgggg
     3421 tcccaatgtc tggtcctatg cgccagccat tcatctcgtc gtcgatctcg gtgtcctcga
     3481 gggctatccg agcgactcga tcgacgggtt caccgacgcg ctcgtggccc tgctgcctgg
     3541 actgcaacgg catacctgct cggtgggtca tgcgggcggc ttcattgagc ggctcgagga
     3601 cgggacctgg ctcggccacg tcgccgagca cgtcgccctg gaattgcagt ccaccgccgg
     3661 ccacgacctg cgtcgtggca agacgcggat ggtcaagggc cggccggggg tctacaacgt
     3721 catcttcggc tacgtcgacg agacggtcgg catcgccgcg ggtgagttgg cggtgcggct
     3781 gatcaaccac ctcgtgcagc ccacggacgg cttcgacttc accgagcagt tggacgcgtt
     3841 cttgcgtcgg gccgaccggg tcgccttcgg cccgtcgacc gccgcgatca tcgaggaggc
     3901 cgtcagccgc gacattccgt ggctgcgcct caactcggcg tcgctcgtcc agcttggtca
     3961 aggggtgcac gcgcagcgca tccgcgcgac gatgacctcg cgcaccggcg cgctggccgt
     4021 ggacatcgcc agcgacaaag acctcaccgg caagttgttg tcggccgccg gtctgccgac
     4081 tcccaagcag caagccgtgc gcacgcggcg cggcgtcctt gacgccgccc gtcagatcgg
     4141 cttccccgtc gtggtcaagc cgctggacgg caaccacggc cgcggcgtct gtctggacct
     4201 gcagaccctc gacgaggtcg acaaggccta cgacattgcc caggagcaat cccgcagcgg
     4261 cgtcctcatc gtcgagtcgt tcgtcaccgg tcgcgactat cgctgcctca tcgtcggcgg
     4321 ccggatgcag gccatcgctg agcgcgtccc cgcccacgtg gtcggcgacg gcgagcacac
     4381 cgtcaaggaa ctcgtcgaca tcaccaactc cgacccgcgc cgtggtgtcg gccacgagaa
     4441 ggtcctcacc cggatcaagg tggacgccgc cgccatcgag gtcctcgaag gccagggcta
     4501 cctcctcacc gacgtcccgg atgccggtgt catggtcaaa ctcgccctca ccggcaacat
     4561 gtccaccggt ggcatctcgg ttgaccgcac cttcgacgcc cacccggaca acgtcgagat
     4621 cgccgaagag gctgcccgaa tggttggcct cgacgttgcc ggcatcgact tcatctgccc
     4681 tgacatcgcg gctcctgtcc gcgagaccgg cggggccatc gtcgaggtca acgccgcgcc
     4741 cggcttccgg atgcacacgc acccgacggt cggcgtgccg cagttcatcg ccaagccggt
     4801 cgtcgacctg ctcttcccgc cgggagcccc ctcccgcgtg ccgatcgtcg ccgtcactgg
     4861 caccaacggc aagacgacca cctcgcggat gatcgcccac atcttcaagg gcttgggtcg
     4921 caaggtcggc atgacctcca ccgacggcgt cgtcatcgac gagcggctgg tcatcaaggc
     4981 ggacgcatcc ggcccgaagt cggcccggat ggtgctcatg aacccccgcg tcgacttcgc
     5041 cgtcatggag gtggcgcgcg gcggcatact ccgtgagggt ctggggtacg accgcaacga
     5101 cgtcgccgtc gtcaccaacg tggcccccga ccacctgggt ctgcgcggga tcgacactct
     5161 cgcccaactg gccgccgtca aggctgtcgt cgtcgaggca gtccccaaga acggcttcgc
     5221 cgtcctcaac gccgacgacc cccacgtgcg gtcgatgcgc cgcaagtgct ccggcgagat
     5281 cgtctggttc acgctggccg cccccggctc ggacatccgc gagttcgtcg acgcccactg
     5341 tcggcgcggg ggtcgggccc tctatctcga cccgaccgat cgcggcgaca tgatcgccat
     5401 ccaacacggg cggcgcgcta tgcaactcgc ctggacccac ctgttgccgg cgaccttcgg
     5461 cggcaccgcc cgcttcaacg tcgccaacgc cctcgcggcg gccggtgcgg cgttcgcggc
     5521 gggcgcgccg ctgcacgaca tccggcaggg ccttcggacg ttcaccagca actactacct
     5581 ctcccccgga cgcctcaacc tcgtcgaggt caacaacgtc gaagtcttcg tcgactactg
     5641 ccacaacgct gctgggatgc gggcgctcgg tgacttcgtc gacaggtatg ccgccactcg
     5701 cgccggtcgc gccgacctcg gtcgggtttc gcgcatcggc gcgatcggtg ccgccggtga
     5761 ccggcgcgac tcggacctgc gcgagttggg tgccgtcgcg gcggagcact tcgacgtgct
     5821 ggtcatccgt gaggacgcgc gactgcgtgg gcgccgtcct ggcgaggcgg cccagttgat
     5881 ccgcgaaggg gtcaaggaag cgatgcagaa gggcgtccgc tgccggcagg tcgacatcgt
     5941 tctggacgag ttgcaggcgg tcggcgatgt catggctcgg gccaaccctg gcgacctcgt
     6001 cgttgtctgc gtcgacaact cggccgaggt gttcgcggcc ctcgaggaca tgacccgcca
     6061 ggcccagccg ggggctcacc ccggcggcgt ctcggacccc gacttcgacc cggtggtcct
     6121 caccggtgcc gccgccgtgt ctggggcagc tgccgaaggc cgggccctgt ccacgccggc
     6181 tcccgaaaca acaggcgcac ccaacgccta gccgggaggg catcaaccaa agttgacgcc
     6241 atcatgccca tcaacgtaga ttgatggaca tgatgnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnncatca accaaagttg acgccatcat gcccatcaac gtagattgat
     6421 ggacatgatg caaccgcggc cacaggccgg gccccaggcc tctgacgtag caccggcggc
     6481 cggggtcgca gggcgtcgcg cccgtcgacc ccgtgccgcg gcccttgtct cggtcctcgt
     6541 gatcgctggc ctgctggctg ccttggtcgc ccccctctcg ctccgcggag gtccggccac
     6601 cagcggcgac ctcgacctcg ctgccagggt gcgtgccgcg gtggaggacg accgcggcta
     6661 ccaggccctg cacgtcagcg agatcacgca aacctcggtg cggcataccg gggtgggctc
     6721 gatcgacccg gggggcgcgg aggcactggg cgctgccacg cccatcgagc tcgggtccat
     6781 caccaagacc ttcaacggac tgttgttggc cgatgccatc acccgaggtg aagtcgcggt
     6841 aaccgacccg gtctcgacct acctgcctcg cctggctggc agtccgatcg gaggggtcac
     6901 cctggaggaa ctggccagcc accgcggcgc cgttccgccc tttccaccgc cgaccatggc
     6961 tcgtgggctt tgggggttgc tcaccaacac cgaccaactg tccggcgacc aactcgactg
     7021 ggtgctcgac caggcggccg cgatgccgct cttcggcggg cgcggcacgg tccagtactc
     7081 caatctcggc gcgaccctgc tcgggtgggc cctggcatca gccgccgagc agccggactg
     7141 gcagacctat gtcacgaccc ggctgctgac gccgctggcc atgacccaca cccgcttcgc
     7201 ggcgacccgt gaccagatcc cgcccgacgc accgcggggt cggttggtcg gtggccgcca
     7261 ggccgtccag ggagtcagtc ccgtctacca gcccgccggg tccagcacgt ggaccacgcc
     7321 cgaagacatc gcccgctacg cccaagccat cctgcggggg accgcgcctg gcctgcccgc
     7381 cctcgatcct cggttcgccg gcgagcaggc cgctagcccc ggcgaccccc gggtcggcta
     7441 ccactggttc acgctgacgg tggccggacg caccgtccag tggcacaacg gcgccacggc
     7501 tggctatacc tccatgctcg tcatcgaccg cttggcgggt cgcgcagtct tcgttgttgg
     7561 caacagcacg cggcccgtcg acccggtggc catccgactg ctcaccggcg tcgccgacgg
     7621 acccggttcg actgacgtcc caatgcccgt gatcgctttg gccgccacca cggtggcgct
     7681 gatcctgatc gcgggagccg gcttcgcggc ataccgggca cggacccgcc tgcaactgct
     7741 gcgagccacc gcgggcgcgc tcctcgggct ctccctctgg cgggtcgccg ggccatggga
     7801 ccacgctccg gggctgctgt ggatcctcgt cgcggccgcc accgcagcga ccgtgatgct
     7861 cgggatcgcg cgggtccgca cccggccctg gcatcgtccc cggctcgcag cgctcagctg
     7921 ggcgggagca gtgctcgccc tcgccgcagc gggcctggtc ctcagcatgg gacttcactg
     7981 agcgcagcgc ggcttagcag ccgctcaggg ccacccgagt tgccccgtcg agttcctcag
     8041 tccgggacac cgggtcggtc ggtgcgcaac tgggcggcgg cgtggtgcaa gaccgcccgg
     8101 tatgccgcgg cgtcctcatc cgtggcgtgc tccatgaggg cgtccaccag gtcagcaacc
     8161 gcctccgccg cgtccccggt gtccacgagc accagcgcgc gcagcgccgc ggcgacgctg
     8221 ctgcccgggt gagtcgctgc gaccgcttcg agcaactcca gggcaatctc cggatgcccg
     8281 agatcgcggt atgccgcggc cgcctgcaac tgggcgcgat ggcggtgtgg ctcgcgcagg
     8341 ccgttgtcca gggcgcggtg atagagcgag accgcccggg cccggtctgc ggtggcatcg
     8401 atcgcacagg cccactcgta ggccagccgt gggtccgacg ggtgggctgt cgccagccga
     8461 ccggcagccg tgacccgctc gccggcatcg gtgagggccc agatcgcggc gatgtcgtcc
     8521 tcggcctcgt gcatgccgcg cgctgcgatg accttgctgc ggatcgtccg cgggtcgtcc
     8581 acggtgccac cccgctgcgc aatgaggtcg gcgacctcgg cccgctggac gtgacacatc
     8641 agcccgatga cgtcaccgct ctcggccgcg gccaacaggg tggtcaaggc ctcgatctcg
     8701 gtgaggaagc ccgcgacctc cgccatgcct gatcgggcca gcccggcgcg cagcaacgag
     8761 tccatctgct ccaggctcat cccccgcagg tatttcagct tgtgcgccag gacgacgtgg
     8821 tcggctccgc ggccggcgat ctcgccgagg gccgcgttgg cctcgggcag ccgatccccc
     8881 gtgccaccga tccccaaccg cacccgccca cccggggcgg tgagcccgcg gcatacctcc
     8941 aggagggctt ccagccccgc ctcgttgtgg gccatgtcga tgaccaccgt cgcggaccca
     9001 ccgccggtga ggggaacgga gtaggtgttg aaccggccgg agttgaggac ggggtcgggg
     9061 cggaaggtcc gcaggccctc gacgaccgcg gcccgggtca ggcccaggcc caacccggcg
     9121 gcggtcccac cgagcgcgtt ggcgatgttg atgctggaca ggcccgcgag ggtcatgggg
     9181 acgtcgacga ccttgacgag acgatcgggg tcacgcccag gggtcaggac gacgatctcg
     9241 ccatccagga cggtgatccc gcggccgccg cccgccaccg cctcccgcag cgccggggca
     9301 ttgggatcga gcgagaaggc ccagatcttg cccttcgccc ggtggcgcat ggcccacacc
     9361 cgcgggtcat ccccgttgag caccgtccag ccgtcgggtt tgacgatgag ggtcgggatg
     9421 gccttgacct cagcgagctg gtcgagggtg tcgatcccgt tgagccccaa gtgatctgcc
     9481 gcgacgttcg tgacaacggt gacgtcggtc tggttgacac ccatcccgcg caggagcatc
     9541 ccgccgcgag ccgtctcaag gatgcccaac tgcaaccccg gcgtgctgag gacctcgcgc
     9601 gccccacccg gcccggagta gtcccccggc tgcaccatct cgcccatgac gacgacgcca
     9661 tcggtcgagc tccacgcggt gcgcagaccg gccgtcatga ccatgtgcgc gacgagccgt
     9721 gtcgttgtcg tcttgccgtt cgtgccggtg atcgcgatga ccgggaccgt cggcgtgagc
     9781 accgcagcgg gctctccgtc aggggcctcg gcgacggcgc gaccggcggc agcgatgagc
     9841 gcctcggtat cggcgtctgc attgagcagg tcgctcagca gcggggcgag gcactccccc
     9901 aacgcgatcg ctcggccacg gtgcctccac gggacggcga ccacgacgtc ttcggtatgc
     9961 cgtccggccc gggtccgcac ggccagtcgt cgcgcgcccg actcggtcgc cacgcggcgc
    10021 acgaggtgct cgacgagccg catgaggaaa cgctggcgct gcccggtgtc gggacgcccg
    10081 gggcgggccg tgcgcagtcc gacgcgacgc gccagatcca gggccgcgtg gacgtcgagt
    10141 tgagcgacgc ccggcagcgc gaggctgacc ttgacggccg gccgggtgac ataaaggttc
    10201 gggccttcga ggatccgaac ttcatcggcg tgcactggct ccatgtcgcg cgagcctagt
    10261 ggtgcaacgg agttcccccg accgatcgac gggaacgctc agcagcccat ggcgtggacg
    10321 ccgccgtcga cgtggacgat ttcgccggtc gtcgccggga accagtccga cagcagcgcc
    10381 acgcaggcct tagcggtggg cagcgggtcg gtgacgtccc agccgagggg cgcgcgggtg
    10441 tcccactgct cctcgaagac ctcgaagccc gggatggact tggcggccgt ggtgcggatc
    10501 gggcccgctg cgacgaggtt gcagcggatg cccttgggcc cgaggtcgcg ggcgcagtag
    10561 cggttggtcg actcgaaggc cgccttggcg acgcccatcc agtcgtagac cggccaggcg
    10621 tagcgcgcgt cgaaggtcag cccgacgatc gagccgcccg gctccatgag cggcagcgcc
    10681 gccacggcca gcgccttgag gctgtaggcc gacacgtgga ccgccgtgcc gacgtcctcc
    10741 cacgtcgcgt cgaggaacgt gaacgcgccc agcggcgcga agccgatcga gtgcagcacg
    10801 ccgtccaggc ggtcggtgtg ttcgcgcagc cgctcggcga gggtgtcgag gtgctccggg
    10861 ttgctgacgt cgagctcgac gaccggcggc gtcaccggga gccggcgcgc gatggtctgg
    10921 gtgatcttca tcgtccgccc gaaggaggtg aggatgaccg aggccccctg ctcctgggcc
    10981 aacttggcga cgtggaaggc gattgaggtg tccatgagga caccggtgat gagcagggtc
    11041 ttaccgtcga acatgcccac gggctggctc gctttcaggt cgggtcacgt caattcagat
    11101 cgggtcaagt caggttcaga tcagggtcaa gtcaggtggc aggtggtcag tggcccatgc
    11161 cgaggccgcc gtcgacgggg atgaccgccc ccgtgacgta gcccgcctcg gggctggcca
    11221 ggaagcgcac cacggcggcg acttcctcgg gcgcggcgaa gcgtccggcc gggatgctcg
    11281 ccaggtatgc cgcgcgccgc tcgtcgggca gcgccgccgt catgtcggtg tcgatgaatc
    11341 cgggtgcgac gacgttggcg gtgatgttgc gcccgccgag ctctcggctg atcgaacggg
    11401 ccatgccgac gaggccggcc ttgctggccg cgtagttggt ctgccctggg gagccgtaga
    11461 ggccgacgac gctggagatg aagatcatcc ggccacgctt gagccggatc atccccttac
    11521 ttgccctgcg ggcacagcgg aaggtacccg ccaggttggt gtcgatgacc gagtcgaaat
    11581 cctcgtcgga catccgcagc agcagttggt cacgcgtcac tccggcgttg gcgaccagca
    11641 cttcaacggg gctgccgaag atgccttcgg cagcggcgaa ggctgcgtcg accgacgcgg
    11701 agtcggtcac ctcgcagggc accgcgtcca gtccttcggg ggcctccccc gacctcgtgg
    11761 tgacgacgac ccgatctccg gcctcggcga atgagcgggc aatggccagc ccgatgccgc
    11821 ggttaccgcc tgtcaccaga acggtgcgcc ccggcgctgc gtcggtcatc gtgaggctcc
    11881 tcgggtgttg gtcgacgtgt gcgaggaacg gtagtcggtt acccctgggt agacccgcac
    11941 ggacgtgccg tatgacgcgt ggctcgcgcc cggtgcgggg ccgaacggaa ggcgacgaca
    12001 cggcataccg agtcgtatgg ttgagaggtg aggctcggac gtaagccgca gacgcccacg
    12061 gtggtccaca cggtgacttc cgcgccgatg tcgcttgcgc aagacactga catgcggatg
    12121 cgccgctacc tcatcacgat ggcgatccgg gtcctgtgct tcgtcctggc cgtcgtcttc
    12181 gaggggtggc tgcggtgggt attcgtcgcg ggcgccgcga tcctgcccta catcgccgtc
    12241 gtcctggcca atgcggtcgg gcccaggtgg ggcgcccgga tctctggcat cgacaagtac
    12301 gagcccaccc acccggagtt gtcggcgtcg acgccgaccg gcgagccggt gttgggcgcc
    12361 gatccgtttc gtcggtgacg ccgaggggga cgactcaccc accgatggct gacatcggcc
    12421 gatccggctg gtggaagtcg ggcctgccga tcccatgggc cgggcccttg cgccacatct
    12481 cgcccctgat cagcccgagc aactccgcgt cactcgcccc gtcgcgcaac ggcccacgca
    12541 ggtcggtctc gcgctcggag aacaggcagt tgcggacctg accgtcggcc gtgagccgca
    12601 cccggtcaca cgcggaacag aagggctcgg agacgctggc gatgatccct accgtcgcgg
    12661 gcccgccgtc aacgaggaac cgcgccgcgg gagcactccc ccgatcggtc gattcgaccg
    12721 gggtgagctc gaaacgcgcc tccaggccgg ccttgatctc ctcggccgtc accatgaccg
    12781 agcgatccca gccgtgttgg gcgtccagag gcatctgctc gatgaagcgc agctcatacc
    12841 cccgggccag gcaccaggcc aacagatcgg cgacgacggc gtcattgacg ccacgcatcg
    12901 cgacggcatt gaccttgacc ggggtcagcc cggcgtcggc ggccgcggcc aggccggcga
    12961 tgacgtcggc caaccggtcg cgacgggtca aggcgaggaa ctgactcgcg tcgatggtgt
    13021 cgagggacac gttgacccgg tcgagccccg cggcggccag cggcgcggcg acggcggcca
    13081 gggcggcccc gttggtcgtc atcgcgatcg cgggtctcgg ccgcagggcg gcgatccccg
    13141 cgaccaactc gacgatgctg cgacgcagca atggttcgcc gccggtgagg cggacctggg
    13201 tgacgcccgc cccgatgaag accccgatga gccgcatcaa ctcgtcgtcg ctgagcatct
    13261 gtggctgagg gatccagggc agcccctcgg ccggcatgca ataggtgcac cggaaggaac
    13321 accggtcggt gagcgagacc cgcaggtcct gcgcgatccg cccgtaggtg tcgatcagcg
    13381 ccgacccctc cgggggtcgg gtgcgggggt cgggcagccg cgtcacaggc gtcaccttac
    13441 gtcggttacg gtgaacgggt gtggcgtgcg atcttcaccc ggcggattct gtatgccgtc
    13501 gcgttcgccc tcggcttcgg caccgtgtgc ttcctgctgg gccagtggca gttgtcccgc
    13561 tacgaagaga aggacgcccg cgccacggcg gtcgagcaga actacaaccg gcctgcagtc
    13621 cccttcgcag gcgtgctccc gtcggtcacg accccgctcg ccgagagcca ggtgtggacg
    13681 caggtgacgc tcaccggccg gtatgccgcg agcacccacc tcatcgtccg cggacgggcg
    13741 ctcaacaccg cccaaggcat ggagatcctc gtccctttcc aggtcgacgg cggcgcggtc
    13801 ctgctggtcg atcgtgggtg ggtgcccaac ggtgaccagg cagcggacct cccgacggtg
    13861 tcggcacctc cggccggaac agtgacgctc accggctggc tcaagcccgg cgaggaggac
    13921 cgcggcaagg acctgcccgc cggtcagctg gcgacgatca acctcgccca ggcccaggag
    13981 caggtgcctg gtcagctcta cgcggcatac ctcgtgctgg ggagcgagtc gctgggtgag
    14041 gggctcccgg cccccgcccg ccccacgccg ttggaggcgc cgacccccga ccgcgggccg
    14101 cacttcgcct acgccctgca gtggtggctg acgacgctgc tggggttcgg cttcgtggtc
    14161 tacctctatc ggcatccacc cggcggcgtc ccgaacgcca cccccaccga ggcgcccgag
    14221 cccaagaagg tgcgcatctg ggacgaagag gatggctgac ggctgacgtc gccgtcaggt
    14281 caccagggac cggccttggt cgtgctgtcc tcgaaatact gcgtgcggta gaggtcggca
    14341 tacaggccgc cgcgaccgag caactccgcg tgcttgcccc gttcgacgat ccgtccgtcc
    14401 tcgacgacga ggatctgatc ggcgccccgc accgtcgaca gtcggtgggc gatcaccagt
    14461 gccgtgcgac cggccaacgc cacatccaga gcgcgttgga ccgccacctc cgactcgctg
    14521 tccaggtgcg cggtcgcctc gtcgaggacg accacgtggg gcgccttgag cagcagacga
    14581 gcgatggcca ggcgctgctt ctcgccgccg gagagccggt ggccacgatc gccgaccacg
    14641 gtgtccaggc cggacggcag cgaatcgacc aggtgcagca cctgggcggc ctccagcgcc
    14701 gcccgcatct gctcctcggt ggcgtcgggc gcggcatacc ggaggttctc gcggatcgtg
    14761 tcgtggaaga ggtgcgcttc ctgggtgacc atccccacgg tctcgtgcag cgatccgaac
    14821 gcggcctctc gcaggtccac cccgccgatc cggacggtgc cgtgcgacgg gtcgtagagg
    14881 cgcgcgacca gtgcggtgat cgtcgacttc ccgccgccgc tcggcccgac aagcgcgacc
    14941 aactgccctg ggtatgccgt gaagctcacc tcgtcgagca ccggcccgcc tccggcgcgg
    15001 tcgccgctgg cggtggattc gagggaacgg agggacactt cgtcggctga ggggtaggcg
    15061 aacccgaccc cgtcgaactc gacggccacg ggcccggccg gcagcgtgac cgggtgggcc
    15121 gactcggcca caagcggctt gaggtcgagc acctcgaaga cccggtcgaa ggacacaagg
    15181 gccgtcatca cgtcgacccg cacgttggac agcgccgtca gcgggccgta gagccgggcc
    15241 aggagggcag cgagggcgag cagggtcccg acggtcaggg cgccgttgac cgccaacagt
    15301 ccgccgaccc cgtaaaccat cgcggtggcg agggcggcga cgagggacat ggccaccatg
    15361 aagacggtgc ggttcatggc gatcttgacg ccggtgtcgc gcacgagctt ggcgcgcacg
    15421 gcatactcgc gctcctcccg ctcgggcgtc ccgaagagtt tgacgaggag cgcgccggcc
    15481 acgttgaagc gttcggtcat ccgggtgccg aggtcggcgt tggcggtcat cgccgtgcgg
    15541 gtgagctcgg acaggcgccg acccatgaac cgagccggga tgaggaagaa gggcaccagg
    15601 gcgagcgccg cgagcgtgag ttgccaggac agggtggcca tggccgcgac gatgagcacg
    15661 agcgacaccg cgttcgacac gaccgaggac agcaccgagg tgaaggcctg ttgagcgccg
    15721 atgacgtcgg agttgagacg gctgaccagc gacccagtct gagcacgggt gaagaaggcg
    15781 atcggctggc gcagcacgtg ggcgaacacc tcggtgcgca gccgcaggat gaggccctca
    15841 ccgatccgcg aggagaacca tcgctgggcg atgctgagga tcgcgtcggc cacggccagg
    15901 gctgcgacca gcagagcaag tcgcacgacg acatccgggg ccttcgggat gacgcccagg
    15961 tcgaccaggt cgcgcagcag cagcggcaca gcgacgacga tgccggcgtc gaggatgacg
    16021 atggtgagga aggcgacgat cgaccgcacg aacggccgcg catagcccag cacccgtcgc
    16081 gtggttcctt gctccaaccg ggcgcgaagg accgattggt ccttcgccat cccgcgcatc
    16141 attcccatgg ggccgccctg accgaagctc atcggcccct ccctccgcgc ctcacgtgcg
    16201 ctcctcgcgg gcgcgtgcgg cgcttctgtg tcgtcgtcac cggcgcaaca ccactggtga
    16261 ctgggtcatt ccgagggtat gccgtcccgc cgcgccgttt cggcgcgaca ggggtcagga
    16321 caggccggca gctcgggcca tgccgtgcag gaggtgaccc tgggctaccc gctcgcggac
    16381 gacctgctcg tcgatcgttg ccgtgagggc gtggttgagc agcggtttga gggcccagag
    16441 cgcggccggc ggggtgccca gcaaggcctg gacgagatcg gaggtcgtcg cggagagctc
    16501 ggtcggcggc acggcgagcg tcgccagccc catccgcact gcttcctcag cgccgacgaa
    16561 ccgcccggtc gcgcacagct ccaaggcccg ggagtagccg accgcctcga cgagcggccg
    16621 cgtccccgcc aggtccggga tcatgcccag gctcacctcg cgcatcgcga acgacacgtc
    16681 gtcggtgaca acccgcaggt cggcggccag cgccagttgg aagccggcgc cgatcgcgtg
    16741 cccctggacg gctgcgatga cgaccggggc aacccggcgc caggccgcga atccctcttg
    16801 gaacggcgcg atgagctcgg tcatcgcctc ggggctgctc gctgcagcgg cgagcaggtc
    16861 gggctcaccc ggcatacctc ccgggcggag catcccgcgg ttgaggccgg ccgagaagct
    16921 gcgtccttcg gcggccagga cgacgacccg cacctcgccc gcgaggccgt gggcgatgtc
    16981 ggccagggcc agccacatgg acggcacctg ggcgttcagg tgctcagggt tgcacagggt
    17041 cacggtcacg accggcccat cctgctcgac gcggatggcg gggtgcggct gcggcaacga
    17101 ggtcacccca ggaggctatg ccctgccctg cagacgtcgc cccgcgggtc agagccgcgg
    17161 gtcgaagccg cgggtcagag cctgtgggcc cagacctgca ggggcgtgtc cccgtgctgg
    17221 cacacggcat acagggtcga tccgatcgcg gtgaacgcct ggaccgtacc ccacgggccg
    17281 ggcccgcggg accatccggt gcgcccttcg acgacgactg tcgcgttgcg cgatgagtcg
    17341 tggtcggtga cgagcaggac ggcgcgtccc tcgacgaaga ccggtgccgg gatgaggtcg
    17401 ctgtcgcgga cagcgacgga gggtatgccg tccacccgcc gagcgccccc atcggcaccc
    17461 acccgccaga cggccagccg gccgtccacc caacctgcga cggtgcactc cgcctcggtg
    17521 caggctgcgc tgacggcctc ccctgcctgc cccgagtcgg gcaggtcgac gcgggtccag
    17581 ccggcgttgc cggtggtgga gcgccagatc gcagggacct tgcgaaccgc cccgtcgccg
    17641 agccgcacga ccgagccggt gatcagggcg ccggatcccc acgcggcgac ggagcgcgcc
    17701 cccattagct gagttggggt gctctccaaa gccgttcccg ccgatggctg tcgcacccag
    17761 gcctcgccgt cgggcaccca agtggcgagg tccaacccgg cctgggcgcc ctcccacgat
    17821 ccgacgagca gcggtccggc cgcagtgagg accgggccga cctgatcgcc cgcgccccaa
    17881 ccaccgaagg tgctgaaggt ctgctcctgc tcggtcaggc cggcggcgac gctgccccgc
    17941 cagaccgtcc agcggacgtt tgcgtgggcc ccgccccgag ccccgccgac cgcaacgagg
    18001 ctgccatcag gtccagcggc gatcgaaagc cattgagcga cgcggccgta gggggtgacg
    18061 gcctggaccg gccgactggt ccaggtgcca tcggccagga cctccacggc gggctcaccc
    18121 gccgcggtcg acctgcccgc cacgagcagg tcaccgaccg cagtcagggt caccactgcc
    18181 ccgacctggg ccgacagtgg cccgagcgcg ctccatccgt cgccctgccc ggctgtgcac
    18241 ccagtcgcgg cagtcaccgt gagcaccgtg agggtcacca gggccctgcg cgccctgggc
    18301 ccccgagggt gtgcgcccga ggtgcggcgc gcggcagacc tcgcggcata cctcgggagg
    18361 gaggtgacca tccccgaagt atgccgccgt gcccatcacg cgaccccggc ttggagtgcg
    18421 cgcggcggac gcgcaaggat gtgccccgag cgccccgacg cgaaggagag tcccgtgagc
    18481 actgtcaagc ggcacaaggt catcctcgac gagcgggaga tgccgacccg ctggtacaac
    18541 atccttcacg atctccccac accgccgccg ccctacctgc accccgggac gggacaaccg
    18601 gtcgggccgg aggacctgct cgcgctcttc cccatggacc tcatccagca ggagatgacg
    18661 acggctcagt acgtcgacat cccgccggag gtcctcgacg tctaccgact gtggcggccg
    18721 tcgccgctct atcgggcgca ccgcctggaa gcggccctgg gcacccccgc gcggatctac
    18781 tacaagtacg aaggcgtctc gcccgccggg tcgcacaagc cgaacacctc tgtgccgcag
    18841 gcgtattaca acgccaaggc cggcatcaag aagctgacga ccgagaccgg cgcgggccag
    18901 tggggcacgt cgctggcctt cgcgtgtgcg cagttcgggc tggagtgcga gatctggcag
    18961 gtccgggcca gctttgactc caagccctat cgccggctca tgatggagac gttcggcggc
    19021 acggtccacc cgtcaccgtc ggagttgacc gcagccgggc gggccatcct cgccgcgcac
    19081 cccgactcgc cgggatcgct gggcatcgcg atctcggagg ccgtcgagat cgctgcccag
    19141 tccgagtcga ccaactacgc actcggatcg gtgctcaacc acgtgctgtt gcaccagacg
    19201 atcatcggcg aggaggcctt gcgacagctg gccaaggtcg aggagacgcc cgacctcatc
    19261 gtcggctgca ccggcggcgg gtccaacttc gccgggctgt ccttcccgtt cctgcgcgag
    19321 aagctcgccg gcaagatgtc gccggtcatc cgagcggtgg agccggcgtc ctgtccgtcg
    19381 ctcacccagg gcacctatgc ctacgacttc ggcgacaccg cggggatgac cccgctgatg
    19441 aagatgcaca cgctgggcca cgacttcatc cccgacccga tccacgccgg tggcctgcgc
    19501 taccacggga tgagcccctt gatctcgcac atctacgagt cggggctcat cgaggcggtg
    19561 tccaagaagc agcttgagtg cttcgcggcc ggtgtgctct tcgctcgcac cgagggcatc
    19621 gtcccggcac ctgagcccac ccacgcgctg gccgagacgg tcgcagaggc gttgcggtgc
    19681 aaggagaccg gcgaggagaa ggtcatcctc acggcgctct gcggtcatgg tcacctcgac
    19741 ctgccggcct acggtgccta cctcgcgggc gagatgagcg accactccta cgaggaggcc
    19801 gagatccgtg acgcggtcgc cgaggcgctg acccacctgc ccaccatccc cggctgaccg
    19861 gcccgactca ttccgcggag ccggcccagc tgaccgggct ggctcattcc gcgcggacgc
    19921 ccgcgggtgc cacgatgagg catgcgtgga cgaactccgg gcggccccgg tgaggaccgc
    19981 aagacccggc gagactggtg gggcgtggtc cttgacgccc cggacgcgaa ggctctcggc
    20041 cagttttatg cagctgtctt cggatgggtg ctcaatgccg aggatcccag tttcgtcacc
    20101 ctcgaccccg gcgagggggt gggctacctc gcggtccaga cctcgcaggg ttacgtgccg
    20161 ccgacctggc ccaatgagcc tggtcgccag cagatgcaac ttcacctcga tatcgaggta
    20221 gacaacctcg atgaggcggt cggcgcagcc gtcgcgctcg gggccaccca ggcggcatac
    20281 cagccgcagc atgacgtacg ggtgttgctg gaccccgcgg gccatccgtt ctgtctctac
    20341 tgcccaggct ggccggttcc gagggcctga cccacccccg gcgcatcgtc agctcgtgcc
    20401 cagcagcgcc tgcgtggcac gcgcgacaac gagtcgcgcc agggcgtcgg tgtcggcgag
    20461 cacccctgcc accgtgtcga ggcccaggcc gagggccgcc gtgatgactc ggtcccgcag
    20521 caccccttcg cagagagcaa ccggcacgac gaccgttggg acgccgtccg ccgcgggacc
    20581 ggcatacccc acgaggtggg tcggctcggt gaccgacgcg agggcagccc acgtcgggcc
    20641 gtccaccgcc gccctcgcga ccgtcagggc ggcgctgagc tcctctatcg cttcgggcgc
    20701 gctagtcccg gcgagggcga ggacgacgtg caggtcccgg acgtcggcga cctccgctcg
    20761 gaccagcaac tcccccacgc cgtcgagcac cgcgcggccg agacccagcc cgctggccgc
    20821 ccgaaaggga cccggcgcag ccgcctgcat cgcctcgatt gccgcaggca cgtccacccg
    20881 cgcgtggtag cccaaggaga gcagcgcagg gacgaccacc cctctcccgg cagcctgggc
    20941 ggcgtcagcg ggggtcggtc cgtggtggtc gaggtaggcg acgtggacgg ccgcctcccc
    21001 cgcagccgcc acccgtgtcg ccagggcggc gatccccgtg gcatgccgcg ggtcgggcga
    21061 gccgtgcgcg agcagaatga tcggctcgcg ctcgctcatc gaaggtcgcc gagcgtctcg
    21121 cgcaaccgca cgacgtcgcc cacgacgacc acggccggag acttcacctg ctcgtgctcg
    21181 accgcccagg cggccttctc cagggtcgtc accgtagtgc gttgggtcgg tgtccaccca
    21241 cgctcgacga acgccacggg tgtcgaggga tcgcgtccgg cgtccatgag ccgagccgcc
    21301 gtctccccga tcccggacac acccatcagc aggacgaggg tggcgtctag gggcgcctgc
    21361 gcggcgaccg caccgccgtc gtgtgccgac gagacgacga acgacgtcgt gatcccccga
    21421 tgcgtcacgg ggatgcccgc ggctgcgggg acggacacgg ccgaggtcac accggggaca
    21481 acctcgacgg gtatgccgtg tgcccggcag tgcagcgcct cctcacctcc gcgtccgagg
    21541 acgaacgggt caccaccctt gagtcgcacg acagtccgac cggcggaggc gtgctcgacg
    21601 aggagggcgt tgatctcctc ctggggcacc ggatgtgcgc cggggcgctt gccgacatcc
    21661 agcacgagga cgtcagggtc gaggtcggcg aggacgtccg tgggaccgag gcgatcggcg
    21721 acaacgacat ccgcagcagc gagcagggcc cgcccgcgga cggtcaacag ccctgggtcg
    21781 ccgggtccgc cacccacgag gtagacccgc cccggcttca tcgtcccggg tacgcgccgc
    21841 acccgacgca ccggaaggac accagactcg aggccggcga ggatcgcgtc gcgcaccgct
    21901 cgggctcgtc gggggtcccg gtcgccggtg acggcgatct gcagaccttc agcaggcgtc
    21961 ccggccgacc cgttcgctac cgccgggctc caggccgccg aacgggcgcc gtcgtccgcc
    22021 cgcacgcacc agatcccaag gccttcgcag tcgtccgcga cctccgagtc gaccgtgtgg
    22081 tccccggtgg cggtgtgcac caaccaggcg cgctgcggct gcatcaaatc ggtcgactga
    22141 tagccccgag cgagccaggt cacccggccc tcggcgacca gtgcggccac gacctcaccc
    22201 acttctggtg ccaccacggt gacgtgggcg ccgtcggcga gcagacggca tacccgggtg
    22261 gccgcaaccc ggccaccacc gacgacgagg acgcggcggt cgcgtaactg cagggtgagg
    22321 gggtagccca tctcagcccc ccgccagacc gcgggaccgg accaagcgtc gttcggccgg
    22381 agcgaagatc agcgactcga ccgcgatgcc gacgaagagg atcagcagga tcgacgccag
    22441 gacgagcggc atgtcggaca gttcccgacc cgtgtcgaga agctgaccca gcccaggccc
    22501 gagttgcggg gagtaggcga tgagctctgc ggccatgagg gagcgccacg cgaaggccca
    22561 gccctgccgc agccccgtga ggtatgccgg tagggccgct gggagggtca cccaccgaag
    22621 ttcggcgagc gttcctgccc ccagcacccg ggcgacccga cggtagctcg gcggagcgtg
    22681 gtcgatgccc gccaacagac cgttggcgat ggagggcacc gaaccgagga ggaccacggc
    22741 gtagacggcg gcgtcggtga gcccgaacca gaggatcgcc gcggggaccc aggcgacgct
    22801 cggcagcgac tgcatcccgg tgacgagcgg gccgatcccc cgtcggatga ggcggaacct
    22861 cgagagcacg atccccagca gcgtgcccag gatgatcgag atcgtgaagc ccaccgcggc
    22921 gcggcgcagg gatccgaacg cggcgcgctg ggcggcattg gactgcagtt gctcgatgag
    22981 ggaggcccac accgcggcgg ggcttggcag ggcgtaggag ggcttgagcc cggacgagac
    23041 gatcacctgc caggccagga gcagcaccac cgcagcgacg agcggcggag ccactcgccc
    23101 caacagggct cgagcgcgcg ggcgaccggg ccctgacggc gtgtcgagga catcgagccc
    23161 ggcttccagg gcctcggtct cgtcgaccgg ggtcccgggt tctgcgggtg tctcggcggc
    23221 cgcggcggcc gcgggtgcga cggcggggac ggcgaggtca gcgggcatgg cgtcggatct
    23281 cctgtcgcaa ttgctcggtg atctccacgg ccaacgcccc gaccgcaggc gactcgatcc
    23341 ggcgggggtg cggaatgggc acggtccact cacggacgat tcgcccagga cggctggcta
    23401 gcagcacgac gcggtcaccc agccggacgg cctcacggac gttatgggtg acgaaaagaa
    23461 ccgtcaggcc ggtctcggcg cggatccgac ccaactcctc gtggagaacg tcccgggtga
    23521 tcgcgtcgag ggcggcgaag ggctcgtcca tgagaagcac atcggcgccc tgggccaagg
    23581 cccgggccag cgagacccgc tggcgcatcc caccggacat ctcgtgggga cgcttgtcgg
    23641 cgtgccgccc cagccgcacc aggtcgagcc accgctcggc ctccgcccgg cggtcgtggc
    23701 ggttgagccg ggcaccgccg cggcgcaggc gcagcgcgag ctcgacgttg ccccgggcgg
    23761 tgagccacgg gaagagcgag gcgtcctgga acatcaacgc gggccgcccg tgcggtgtga
    23821 cgatgcgacc gcgggtcggc cggtccagcc cggcgaccat cccgaggagc gtcgacttgc
    23881 cgcacccgga ggcgccgagg accgcgacga gttcgccctg ggccacctgg agggacaggt
    23941 cctcgatgac cgggcccgtc gctgcgtcga aggtcttggt cacgccctcg atcgtcaccg
    24001 cgggggtgcc ttcgagagtg tcgccggcaa cccggccgac gactgtcgtg gcatcggcct
    24061 ctgccggctc ggaggccgcc gcggaggaca gggtggtggc gaggctcatc gtcactcctt
    24121 ccccagcccg ccagcactca ccgtcgggcg acccgaggcg gtgaggacct ggttgagcag
    24181 ggtgaggtcg tagatcccgg acaaatcagg gggggacttg gtgatcccga cagccgcggc
    24241 cgctgtggcg ctggcctgca gcgacgacgc gatggggtcg ggggtgatcg tcaggcgtga
    24301 ccaggcctcg tggaggacct gggtcgacag tttggcgccg gtgagcgccg tgatcgcgga
    24361 accaacgctg tcccgggatc cctcgggatc ggccgcaatc gccgcgtcgg cggcgaccgc
    24421 accctgcagc agtgccttga cctgctcggg gtgcgcctgc agataggtgg tggagacgac
    24481 caggttggtg gtgacgaacc tgccctgcgg ccagagcgtc gcctcgtcga cgagcgtcgt
    24541 cgccccggcc tccaaccgca gccgggaggc ccacggctcg ggcagccagg cgccgtcgac
    24601 ggccccggcc ttgaagaggc tgagcgtctg ggcgttgctc tgcggtgcga ccaggacgtt
    24661 gccgcctccg gtcaccgtcg tcgtgtagcc gtgggccgac agccacgacc gcagcgcgac
    24721 gtcctgggtg ccccccagtt gcggcgaggc gaagaccttg tcggcgagag tcgactcggt
    24781 gagccccggc cgcacgacga acgacgcgcc gccgctcgtc ccgcctgcga cgatccggat
    24841 cgcagtcccc tgcgacttgg agaacgcgtt gatcgccgga ctcgggccga ggtatgccgc
    24901 gtcgatggcc cccgcgagca aggcctcgac cgccgcgggt ccggcgttga agacctgcgt
    24961 ctgcagtgtg gtcgtgccga gcgctgccgc gaagtggccg tccttcactc caaggactgc
    25021 ggtcgcatgg gtgaggttgg cgaagtagcc caaccgaagg gtgttggcgg aaccgcccgc
    25081 ggcggcgccc actcccgtcc cactggccat gacggagccg gtcgcggtgc agccgctgac
    25141 ggctagcacc ccgaccaccc cgagcagcgc gagcaacttc attcctgggc catcccggcg
    25201 gccagcgtcg tgccgtcgac ctcgtcgatg agcaggaacg ccccggtgtg ccgcagtcgc
    25261 cggtagtcgt ccagcgccac gggctcggcc agtcgcaccg tcacccggcc gatggcattg
    25321 agccccaacg aatcacccgt ggggaggtgg gtcatcgtcg tgatgtccaa ctcgtcgtcg
    25381 atcgactcga cgatgccgcg gacggtccgg gtgccgaccc tggcgagaac ccggtcacgc
    25441 acccgcagcg gccggtcgga gaggaccgcg acgggcccgg agagggcgcg gcataccgtg
    25501 ggctcatcct gcggcgaagc gatgaggtcc ccgcgggcaa cgtcgatgtc gtcggccaag
    25561 cgaagggtca ccgagcgggg cgcggccgcc tcggccacgg acacgacctg accgtcaacg
    25621 gcaggcaggt cgacgccgac cacccgcgag cgacgcccgg aggggtggat gacgacctcg
    25681 tcgcccacgc ggacgacccc tgaagtgagc cgaccggcat acccccggta gtcgggatgc
    25741 gccggggtcc gcggccggat caccaactgg accggcaggc ggaagggctg ctcgctcggg
    25801 tctgaaccga ccgggacctc ttcgaggtgc tgcaggaggg tcggaccggc ataccagggc
    25861 gtgtgctccg agcggtcgac cacgttgtcg cccagcagcg ccgagaccgg gatggcggtg
    25921 acgtccgaga tgccgaactc ccttgccaag gtggtgaatt cgtcggcaat ccggtggtag
    25981 accgacgggt cccaaccgac gaggtccatc ttgttgacgg ccagcacgac gtgccgcaca
    26041 ccgagcaacc ccgtgaccgc gaggtgccgc cgggtctgtt cgaggatgcc gtgccgagca
    26101 tcggtgagga tgagcgcaag ctctgcggtc gacgccccgg tgaccatgtt ccgggtgtac
    26161 tccacgtgcc caggagtgtc ggcgaggatg taggagcggg tggcggtcga gaagtagcgg
    26221 taggccacgt cgatggtgat gccttgctcc cgctcggcgc gcaggccgtc ggtgagcaga
    26281 gcgaggtcgg tggcctccag gccccggtcg cggctcaccc gctcgacggc ggcgagttgg
    26341 tcggcgagga ccgacttggt gtcgtgcagg aggcgaccga caagggtgga cttgccgtcg
    26401 tcgacgctcc ccgccgtggc gaagcgcagc aactcgtgcg gcacggcctc gatggtggca
    26461 gtcatcagaa gtacccctcc ttcttgcggt cctccatggc ggcctctgac aaccggtcgt
    26521 cggcccgggt cgcaccgcgc tcggtgagcc gtgagccggc cacctcaagg acaacgtcgg
    26581 ccacggtgag cgcgtcggaa tcgacggctc cggtgcaact catgtccccg accgtccggt
    26641 aacgcacggt gcgcacctcg ggcgtttcgc cagcccgcgg gccaccccac tccccggcgg
    26701 tcagccacat cccgtcgcgg aggaagacct cgcgctcgtg ggcgtagtag aggtcgggca
    26761 gctcgatgcg ctcgcgctcg acataccgcc acacgtccac ctcggtccag ttcgacagcg
    26821 ggaacacgcg cacgtgctcc cccggcgcgt ggcgaccgtt gtagaggtcc cacaactccg
    26881 gccgctgccg acgcgggtcc cacgcaccga aggcgtcgcg gatcgagaag acccgctcct
    26941 tggcgcgcgc cttctcctcg tcgcgtcgcc cgccgccgag cacggcgtcg aagcggttcc
    27001 cggagatggc gtccagcagc ggcacggtct gcagggggtt gcgcgtcccg tcggggcgct
    27061 cgcgcagtcg tccgtcgtcg atgtagtcct gcacggacgc cacgaccagg cgcacgccca
    27121 gccgcgcgac ggtcgcgtcg cgatagtcga ggagctcagg gaagttgtgc cccgtgtcga
    27181 cgtgcagcag cgcgaacggc agcggcgacg gcgcgaaggc cttcacggcg aggtgcagca
    27241 tgacgaccga gtccttgccg ccggagaaga ggatcaccgg ccgctcgaac tgcccggcca
    27301 cctcccgcac gacgtggatc gcctcggcct cgaggtggtc cagatggtcc agccggtatg
    27361 ccgcgcgccc ggcactcggg cgcgccgtcg cctcggcggg tgtctcaact gcagggggcg
    27421 tccccaccac ggcggtcact ggtgcaaccc gcattcggtc ttggcgcgcc cggaccagcg
    27481 accggctcga gggtcttccc cctcggccac cggccgggtg cacggctcgc acccgatgga
    27541 ggcatagcca tccaggcgca aggggttctc catgacggat tcctctcggg tgtaacggtc
    27601 gacgtcggca gccgtccagg cggccagcgg gttgagcttc accaggtcgc gccgggcatc
    27661 ccagccgacg agggcgatgt cggtgcgggt cggggcgtcc tcgcggcgca tccccgtgac
    27721 ccaggcctgt agccctccga gtgcccgttc cagcggctca accttgcgga tggcgcagca
    27781 cgcgtcgggg tcgcgctcga agagccggtc gccatgtcgc tcggcctgct cggcaatggt
    27841 gagcagcggc agcacggtgc gcagccgcac ctcgccggtc gcggcatacg cgtcgcgcgt
    27901 gccgagcgtc tccgcgaagt ggtagccggt gtcgatgaag aacacatcgg tcccggggac
    27961 ggtctgcccg acgaggtgca ccagcacttc gtcgcccatc gacgacgcga cgctcaggcg
    28021 gtcgccaaag atcaggcggg cccagcgcag ggccacacga gcggcctcga cgcgatcctc
    28081 gtatgccggt ccgtcgccgt ccccgaccag cgcagcaccc tcgtgggcga gttcgcgcag
    28141 gtcggtcacc gaccgggtcg cggcgctcat cgcccgctcc tgcgaatggt gacgctgaag
    28201 accgaggcgc aggcacggca tacccacgac tgctcgtcgg tctcgtcggg gcgcaggtcc
    28261 tgctctccgc acacggggca gaagtagggg gtgccgctca tcggagggcc tcctcgtcgg
    28321 cgcggtgggc ccattcggcg aaggactcgc cgggggcctg gtcggtgcgg aagcgggtga
    28381 cgacgtgctc gacgtaatcg acgaggtcgt ccgcctccac ccgcaaggcg cgggtcttgc
    28441 gagccagccg ggcgttgccg ccgagggcgc ccccgaggtg gacctggaag ccctccacgg
    28501 tctcccccga ggcccccgtc atgaccaggc ccttgaggcc gatgtcggcg acctggaaac
    28561 gggcacacga gttggggcag ccgttgacgt gaatggtcac gggggcgtcg aggtcgggga
    28621 tccgacgttc gagctcctcg acggccgatc gggcgcgggc cttggtctcg accagcgcaa
    28681 gtttgcagaa ctcgatcccg gtgcaggcca ggactccgcg gcgccactcg ctggcgttgg
    28741 cggggaggcc aagggcgtcg agttcgacga cgagcgtcgc cacctcggct tcggggacgt
    28801 cgaggacgag gagcttctgg tgggccgtca ggcggacacg gcccgagccg tgcctggcgg
    28861 ccaggtcggc gaccgcagtg agggtcgtgc cggagacgcg cccgccgatc ggagccacgc
    28921 cgacccagaa gcggccatcc acctgggggt ggacgccgag gtggtcacga cggcgttcgg
    28981 ggtcctccac gggagccggt ccgtcgagga gtcgccgtcc gaggtattcg gtctcgagga
    29041 cctcacggaa gcgttcggct ccccagtcgg agacgaggaa cttcaaccgg gcccggttgc
    29101 gcaggcgccg atagccgtag tcacggaaga tgcccacgac ccccgcccag accgctggca
    29161 cgtcctcgat cggcacccac gcgcccaggc gctgggcgaa gatcgggttg gtcgacaggc
    29221 cgccgccgac ccagacgtcg aagccggggc cgtgctcggg gtggaccacg ccgacgaacg
    29281 acacgtcgtt ggcctcgtgg gcgacgtcga ggctcggcga gccggacacc gcggtcttga
    29341 acttgcgcgg caggttggag aactccggcg aaagcaggta gcggtcgagg atctcgtgga
    29401 tggcgggagt gccgtcgatg atctcgtgga cgtcgatgcc cgccaccggg gagccgagga
    29461 tcacccgcgg aacgtcgccg cacgcctgag cactgttgag ccccacggcc tcgatccgcc
    29521 gccagatctc cgggacgtcc tcgacgcgga tccagtgcag ttggatgttc tgtcggtcgc
    29581 tgatgtcggc ggtgtcgcgc gcgaactcgg tggagatgcc ggcgatggtg cgcagttgga
    29641 cctcagagag ggcgccaccg tcagatcgga tccgcagcat gaagtaggag tcgtccaact
    29701 cgtcggcgct gagcttggcc gtctgcccgc cgtcgatgcc gggacggcgc tgcgtgtaga
    29761 gcccccacca acgcatccgg ccgcgcaggt cgtccacggg gatcgacccg aagccctcgc
    29821 gggagaagat cgtctcgatc cgctgccgga cgttgaggcc gtcgtcctcg agtttgaagc
    29881 gctcgttgtc gttgagcggc gtccggtcgc cgtcggccca ggccccagtg gcgcgggcgg
    29941 cggtcgtgag gggtgtggac atgcgtcagt gatcccgtcg ggtgggagga gaagaggttc
    30001 aggcacgcct gcgtgccagc gccggtggcc atgacggctc ggcaggtggg ccaggtgggc
    30061 gtcggccggc aaggggtatg ccgcggcggc gcgtctggcg gatcagtccg gtatgcaggc
    30121 cctacctggc agacagcgac tcggagagtc agccgcgccg gagggccaag ggcatgtcgg
    30181 aggcgagccg gcaccgtcga catccggcgc tggcgacccg aagaaggtcg acatggcgtc
    30241 gggagacgag gcgtgcaccg ctcatgactc catgatgggg cactcacccc tcatttgcat
    30301 aatggtccat tatgtgaact caagcgtttg gttattcaca tgggacagct tcgaacggcc
    30361 gctagcgcag gctaggcgaa ggctcgtgaa ggtcacccct tgccatcacg ggtgggggcg
    30421 cgcggagtgc gcctcgaatg cccacgacat cttcgagact tcctgcccgg aaaggccata
    30481 ctggcgggcg accgtggccc actgcgagac gaccgcctga acatgtacga cgacgtcgtt
    30541 ggccgcctca gcaccgaggc ggaagtagtc cgcgacagag agcgcaagcg agatgctcgc
    30601 cgtgtcatcg gcgaggtcga tggccgtgct cagatgtttg ggtcccggtt cggggttcgg
    30661 gttgacgtcg aacgctggag acaaccgcca ggcgtcgccg cccgcgtgga ggaacccgtg
    30721 gttgcgcagg tggtcgtcgg tgttggagat gaggatggag aagacgatgc ggcgccagag
    30781 ctggtgcagg tccgtcgtag ccttcgacga gcgcatctcg atggcttcgg ccatctccag
    30841 gtagctgccc tgctctccat cggtggaccg cagcatcgac atcgcgctca cgtagccgac
    30901 ccggccggtg gctccgccgt cggcatcgcg ggtccggtcg aaccggtcca cgatgagaac
    30961 gctgcggtcc gcgaggttca gcagagtact ggccggaacg gtgatcccgg cccgggcggc
    31021 aagttccagc gcgaccttct cccacgccat gacgttccac gtgtcgtgcg ccgcgctggg
    31081 gaacttggcg atggcgaggg tgccgtcggc gccgcgaacg tgcgctttgg gccgcgcccc
    31141 gcccagagag ctgccgacgc ggatgaggcg ccgaaggtct ggcaggtcgg ctgagtccgc
    31201 ctcggctcgt gcggcgaggg tgagcagctc agggaggtcg gtcagtgcag gcacgccacg
    31261 gtcccccacc gcctcgaacg gtccgtcacc acggcggaag cgcagggcgc cctgccgcag
    31321 gtcgtcgcgg accccgagga ggtagtcgac ctcaccgagg ctgcgcggtt cgcggcctgc
    31381 ggctcgagcc tgggcggcct cacaccgttt gaccagagtc cggccccatc ggtccggggc
    31441 gcagtccgtg aacgcgccga acatcggatg ccccagggtg ctctggtgag ccccggcctt
    31501 gagtggcagt cccggctcca gggagtaagc gcgcgggttc gcgatgtact cgtccacgta
    31561 ggtgaagctg gcactctcgg tggcgccacc gcggcggcgg tgcgggtaca gcgtgcccgc
    31621 gcgcacgtcg acgccctcga tggacaccca gacctcgaca gcgctcacct gcttactccg
    31681 cgcgcccgca ccctggtggg gagcttctct tcggcacgga ggcgacccac gtcggtggcg
    31741 tacgggtcga gggccttggt gagcagttcc agcacaccca gcgcgcgagc cacccgcagc
    31801 aggttctcca cggaagctcc ctgtccgcct tccaggcgca ggacggtgct ccggctgacg
    31861 ccggcgcggt ccgcgacctg ctcggccgtc aggtggcgca acttgcgcca tgtggtcagg
    31921 tccctgccca gcccgtccag cgcgcgcgct accgccacag gggtgcgccc agtcgtcgcg
    31981 gcagtcatat gtgcccttaa gtttcagatc tgttgcttta acacgcttag cgtagcaaat
    32041 ctgagctccg aaagacgccg ccctgcgcaa ggcaccacgc tggcggggtt gcccggataa
    32101 ggagacccgc tacgcgagcg agacgaggtc cttgtagtcg gcgccccaca ggtcttcgac
    32161 tccgtcgggc aacagcagta cccgctcggg ttcgagcgcg tcgaccgcac cctcgtcgtg
    32221 ggtgacgagg acgaccgcgc cttcgtaggt ccgcagtgcg ccgaggatct cctcgcgcga
    32281 ggccgggtcg aggttgttgg tcggctcgtc gagcagcagg acgttggccg ccgacacgac
    32341 gagcatcgcc agcgacaacc gcgtcttctc tccgccggag agcaccttgg ccggcttgtc
    32401 gacgtcgtcg ccggtgaaga ggaacgagcc gaggaccttg cgcacctcgg tctcgccgag
    32461 atccggtgcg gcggtcttca tgttctccag cacggtccgc tcgacgtcga ggttctcgtg
    32521 ttcctgcgcg taatagccga gtttgaggcc gtgccccgga agcacctcgc ccgtgtcggg
    32581 agcgtcgacc ccggcgagga tgcgcagcag cgtcgtcttc ccggcaccgt tgagcccgag
    32641 cacgaccacc cgcgagccgc gatcgatggc caggtcaacg tcggtgaaga cctccaagga
    32701 cccgtaggag cgcgagagcc cctctgcagt gaggggagtc cgaccgcacg gtgcggggct
    32761 cgggaagcgc agtttggcca cccggtcgtg ggcgcgctcc ccctcgatgc cggccaacag
    32821 tcgttcggcc cgacgcgcca tgttctgggc agccgtggcc ttcgtggcct tggcccgcat
    32881 cttgtcggcc tgggccagca gggctgacgc cttcttctcc gcgttgagcc gttcgcggcg
    32941 ccgccgacgt tcgtccgtct cgcgctgaag gaggtagggc ttccaaccga cgttgtagat
    33001 gtcgatctcg gaccggttgg catccaggtg gaagacccgg ttgacgaccg tgtcgagcat
    33061 cgccacatcg tgggagatga tgaccagccc gcccttgtag gacttgaggt agtcgcgcaa
    33121 ccagccgatc gagtcggcat cgaggtggtt ggtgggttcg tcgagcagca aggtctgggc
    33181 gccggagaag aggatccgcg acaactcgac ccgtcggcgc tggccaccgg acaacgtccg
    33241 taggggctgg cccaacaccc ggtccggcag ccccagcgac gaagcgatcg aggcggcctc
    33301 cgactcggcg gcataccctc cttgggcgtt gaactccgcg tccagccgcg tgtacttgcg
    33361 catggcgcgt tcctgcaccg cgccgtcgct cgatgccatc gccgtctcgg ctgcccgcaa
    33421 ctcgcgcagc gtgacgtcca ggccacgggc ggcgaggatg cgatctcggg cgagtacctc
    33481 aaggtcaccg gtccgggggt cctgcgagag gtagccgacg gcgccggtgc gggtcacgct
    33541 gccggaggtc gcgagcgcct caccggcgag gaccttggtc agcgtggtct tgccggcgcc
    33601 gttgcgtccg accaggccga tccggtcgcc ctcggagatc cgcagcgacg tctccccgag
    33661 caacaagcgg gcgcccacgc gcaactgcac ggcggtggcg acgatcacgg catacctctg
    33721 ggtcgggggc gccgggaacg gcgacgacaa gctggacctc cctattctgc caggcgcggg
    33781 caccgggacc gacccgcaac cgctcttggg gctcgccgga cgtgcccgag tcgtgaccct
    33841 ccgcgggccc gtcacgggaa ggcagcgcac gctctggccg ttagggtgtg tgggtgacgc
    33901 ggcggtccat ccgcccacat gactggaggg gtgcatgtcc ttcaacgaga acgtccaact
    33961 cgac
//
LOCUS       DAOMZY010000004        33083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_1005_length_33083_cov_6.416465, whole genome shotgun sequence.
ACCESSION   DAOMZY010000004 DAOMZY010000000
VERSION     DAOMZY010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 33083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33083
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1005_length_33083_cov_6.416465"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PLK64_00485"
     CDS             complement(<1..478)
                     /locus_tag="PLK64_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78245.1"
                     /translation="MADKKELTADQQAELDEIFARARAALAIIETYDQARVDRLCQAV
                     AWAVSNKVTFTRLVAEGIEESGLGDPVSRMGKRMKIRGVLRDALRQKSVGIIEEIPEK
                     GLVKYGKPAGIIGCIVPTTNPDLTPAGNAIYAIKARDVVIFSPHPRSKKTTFETVRL"
     gene            693..1331
                     /locus_tag="PLK64_00490"
     CDS             693..1331
                     /locus_tag="PLK64_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HPV78246.1"
                     /translation="MSHQSSAALLALHAVRVTGMASTERAAARYGLPQAEAHELLEDD
                     RARGYVRRVEFVDLAGWTLTESGRTEDERRLAAELALTGARPVVQAGADDFVGLNARL
                     LTAITDWQLRPQPWDRLAANDHTDHRWDDRVLRELTRIGELLAPVCDPLAAALSRFEG
                     YPQRYAAAMARVADGERSWVDRVGPDSAHGVWLELHEDLRATLGAGPCVTPS"
     gene            complement(1318..1758)
                     /gene="aroQ"
                     /locus_tag="PLK64_00495"
     CDS             complement(1318..1758)
                     /gene="aroQ"
                     /locus_tag="PLK64_00495"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011693217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HPV78247.1"
                     /translation="MSVLLINGPNLNMLGTRQPEIYGSTTLADVEAAVRAQGEAAGVS
                     VECFQSNYEGAIVERLHAARTDGTAFIIINPGAFTHTSVAIRDAFAAAEVPFAEVHIS
                     NVHKREPFRHHSYLSDIAVGVMAGFGTLGYNLAMTFALQELKTA"
     gene            complement(1822..2904)
                     /gene="aroB"
                     /locus_tag="PLK64_00500"
     CDS             complement(1822..2904)
                     /gene="aroB"
                     /locus_tag="PLK64_00500"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HPV78248.1"
                     /translation="MADLVRVGVGDDYDVLVGRGIATFAVDLLADWVAKVLVVHAPTG
                     RDLAALLAARIEAGGRTAYLHEVPDAEAAKTPIVAAQAWGVLGQAGFTRSDAVIGVGG
                     GAVTDVAGFIAATWLRGVAVVQVPTSLVGMVDAAVGGKTGINTAEGKNLVGSFHPPAG
                     VVCDIATLNTLPRAELVAGMGEVVKHGFIADPRTLELIEADPAAALDPAGDVLAELVE
                     RSIRVKAAVVAADLRESSLREILNYGHTFAHAVEQVEGYSWRHGNAVSVGLVYAAALG
                     RLAGRTPADVAARHTEILTSLGLPVTYPSAGGQAWPRLLTAMRRDKKSRGALLRFVVL
                     DDLATPGRLEGPDEALLEAAFAELAS"
     gene            complement(2897..4495)
                     /locus_tag="PLK64_00505"
     CDS             complement(2897..4495)
                     /locus_tag="PLK64_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional shikimate kinase/3-dehydroquinate
                     synthase"
                     /protein_id="HPV78249.1"
                     /translation="MIVVVGFMGAGKTTVGRLIAERLGLPFVDSDLVIEHQQRRSIKD
                     IFAAEGESGFRDVEAATIAELLDGPACVLSLGGGACGRAETRERLRGHTVIYLHVELD
                     EALARVGRDEYRPLLHDAGLPALYAARLDIYAATATITSRTTGRRVEDIGLEIIDAIT
                     GTAALDGTAGVLVAPGGGSYRVHVGRGIRSQIAALLPPMPDAEQIVILATADEGEAAA
                     EIVAQLRTLAIPVRRVTLPSGSTAKTLDAVGLAASALAELSVHRGDLLVGVGGEATCD
                     VTGFLAATYNRGMAHALVPTTLEAQADSAIGGKCSIDLPHGANLLGTIHQPVLVVCDI
                     ELAVLSATYGDADYRAGLAEIVKHALLEDPALLDTLRERRDAIVARDGQTLVEIVRRS
                     VEIKAAVVTADEREQGGRVHLNYGHTFSRAFARVPDPQVVDGALALGLMAAAHLSHRL
                     GRLDADGVAAHRDTLAALGLPTSARISLDDLVDALARDKKYRKGWRFVLLDALGRAQA
                     GVSPDVEQLAGALDDLAVGASEHG"
     gene            complement(4509..5732)
                     /gene="aroC"
                     /locus_tag="PLK64_00510"
     CDS             complement(4509..5732)
                     /gene="aroC"
                     /locus_tag="PLK64_00510"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPV78250.1"
                     /translation="MLRWLTAGESHGPALIAILEGLPAGVSVSRADLGRALARRRLGF
                     GRGARMAFEQDDVEFLGGVRHGFTLGSPIAVRVGNSEWPKWQTVMSPEPVDADAYAAA
                     NDVNAEKEIARNRPLTRPRPGHADLVGMQKYGFDDARPILERASARETAARVALGEVA
                     ARFLEQTVGIRLVSHTVAIGTAAVADTAALPHPDDVDRLDADPVRTLDAEGSAAMVAE
                     VEAARRDGDTLGGVVEVLAYGVPPGLGSHVHWDRRLDARLAGALMGIQAIKGVEVGDG
                     FETAKRRGSAAHDEMARRDDGVIERRTGRAGGTEGGMSTGQILRVRAAMKPISTVPRS
                     LDTIDVASDEPAKAIHQRSDVCAVPAAGVVAEAMVALVLAEAVVEKFGGDSVAETARN
                     HAAYLAAVPERMRTW"
     gene            complement(5790..6464)
                     /locus_tag="PLK64_00515"
     CDS             complement(5790..6464)
                     /locus_tag="PLK64_00515"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HPV78251.1"
                     /translation="MTVFTQTGPWWLVLAAVVVGSALGGWLRRELVTGGYRLDDEWDR
                     PVLRRPWLLVALVPLVWGLLAWRVGGPGTLTLLPALLLVGFAGVALSWIDIDVHRLPH
                     GLTYPLGAGVLGIVLVAGAVSGSWTLPVRALVSGVAAYLVLLVLALISRGQFGLGDVT
                     VGGILGIALGSVDGRLPWWGLMLAFVISGLVSVVGLATRRLTMRTDLAFGPYLLVGAL
                     VAVLLG"
     gene            complement(6490..7311)
                     /locus_tag="PLK64_00520"
     CDS             complement(6490..7311)
                     /locus_tag="PLK64_00520"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPV78252.1"
                     /translation="MRSVVHNRCGVRGSPIEHSLSPVLHRAAYRALGLGDWSYDRIRV
                     EADELATHVAALDETWRGLSLTMPLKEAAFDVVHTASDLAVQVGAINTLVRDEAGWSG
                     HNTDVYGIVEALREAGVGHVRSAVLVGAGATARSALAALADLGAAQVSFMVRDAVRPG
                     TLAFARDLGLDAVTVPMGHWPYGTDVIVSTIPATAYAGLLGALPEAHPGSAVLDCVYG
                     DGPSPLLGVAQEIGYAVVGGTDMLLHQAREQVRLMTSRDAPTEAMRAALLAAADV"
     gene            complement(7308..8708)
                     /gene="mltG"
                     /locus_tag="PLK64_00525"
     CDS             complement(7308..8708)
                     /gene="mltG"
                     /locus_tag="PLK64_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPV78253.1"
                     /translation="MTERPLRDPRGDDVWGQAAGEVAGEHEVHAGHEGYDAHEVDYSG
                     AGGYAHEGPDVHEGEYAHAGNDAHEGEYAYEHEYEHDGVEEGHGGYHDPDAAAFGFAE
                     DSRRARRERRNTGRGVRRFLILFVALGLIAGAGFLGFTALRPLFDRSADASDFAGPGG
                     AAARFTVNDGDTGRAIGSNLQTQGIVLTAKAFVDACSADSRCAGIQPGDYALKKEMKA
                     ADALAILVDPKNRQNPKVTVREGLWASEIYALLGKATKHSVADYEAAAKSPDVLALLP
                     PSAGGNVEGYLFPATYEFSDKDSAVTQLTAMVGKTVKVLTDLQIQPDQAERIMIIASI
                     VEAEAKRSEDRAKVARVVLNRLAKPMRLQMDSTVSYGVKKRAITTTDEERASVNGWNT
                     YTKDGLPDSPIANPGLASIQAAVAPAEGPWLFFVAVNPETGETKFAATQAEHDTYVAQ
                     FQAWCNAAENQGKCSR"
     gene            complement(8705..9205)
                     /gene="ruvX"
                     /locus_tag="PLK64_00530"
     CDS             complement(8705..9205)
                     /gene="ruvX"
                     /locus_tag="PLK64_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPV78254.1"
                     /translation="MPSQGRRVGVDVGSVRVGVAESDPDGILATPVTTLARDESPEHA
                     DLAALAALVRDRDATCVYVGLPLQLSGVSGESARQARAYAAELAALVAPVRVRLVDER
                     LTSVASHRLLHDSGRAERTHRAVVDQVAAVLILKSALEAERATGSQPGELVSTGGRKP
                     RTKGRA"
     gene            complement(9212..11914)
                     /gene="alaS"
                     /locus_tag="PLK64_00535"
     CDS             complement(9212..11914)
                     /gene="alaS"
                     /locus_tag="PLK64_00535"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPV78255.1"
                     /translation="METAEIRRRFLAFFEGRGHTVVPSAPLLYDDPNLLFVNAGMVPF
                     KPYFLGQETPPFDRATSVQKCVRTGDIEEVGKTSRHGTFFQMNGNFSFGDYFKKGAIK
                     AAWELLTTPQQQGGYGLDGDRLWATVYLDDDEAADLWVKHTGIPRERIVKRGMADNFW
                     SMGVPGPCGPCSEIYYDRGPDYGREGGPAVDEDRYLEVWNLVFMQNIRGTGGAKEGYE
                     ILGDLPAKNVDTGMGLERMAAILQGVDNLYEIDEVYPVLDKAAELTGKIYGANSGRDA
                     GHSHPDDVRLRVVADHVRSSLMLIGDGVTPGNEGRGYVLRRMLRRAVRSMRLLGYDDP
                     ALPHLLPVSMERMSKSYPELRTDFGRISTIAYAEEEAFRRTLASGTTLLDGAVARLKG
                     TGGEVLTLPGDEAFALHDTYGFPIDLTLEMAAEQGVQVDEAGFRRLMKEQRDRAKADA
                     KAKKSAHGDTGVYREVADRLGRGVEFTGYDEVVSEARVAGIVRDGRSVGQAIAGEDIE
                     LVLDRTPFYAEGGGQLADGGRIELANGAIVQVRDVQSPITGLSVHRATIIAGEVTVGE
                     QAQAEVDVERRRSISRAHTATHMVHKAIREALGDTATQAGSENGPGRFRFDFNAQHAI
                     PLGQLQEIEARVNAVLLDDLPVSAEVMTQDQAREIGAMALFGEKYGNAVRVVSVGDWA
                     RELCGGTHTPRTSQLGVVKLLGEASIGSGVRRVEALVGADAYSFLAREHAIVGQLTEA
                     LKARPEELPERISGILAKLRDAEREIAAARQQQALAVAPGLVSSAREIGSVRLVTHDA
                     GEGIAPDDLRSLVLDVRSRLGDAHATVVAMTGSMGGKPVVIVATNAPARQGGVKAGAL
                     VRLAAQVLGGGGGGKDDLAQGGGVDAASMGASLGAVEDSLRAQG"
     gene            complement(12026..12325)
                     /locus_tag="PLK64_00540"
     CDS             complement(12026..12325)
                     /locus_tag="PLK64_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78256.1"
                     /translation="MSRKLLVLATGALVGVGAVYAYRLLADRYSTEGGPPDVLAAALA
                     EAGAVVADIRAGMTEREEQLRLALGLDSPPSGTRLDPQATRDLLDDPAGWRARQG"
     gene            complement(12322..12720)
                     /locus_tag="PLK64_00545"
     CDS             complement(12322..12720)
                     /locus_tag="PLK64_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012245384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HPV78257.1"
                     /translation="MTIGDIAGVIAALAFAYLVWRLGSVVGKAGEILDETRIGVKGLA
                     DGSVPLLGEVTTTVSTTNEQLARIDVVTTNIASMSSNVNALTSLFAATLGSPLVKVAA
                     FSYGVRSAVGQRRATTAASGGSTRKGRHEQ"
     gene            complement(12799..13863)
                     /locus_tag="PLK64_00550"
     CDS             complement(12799..13863)
                     /locus_tag="PLK64_00550"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HPV78258.1"
                     /translation="MTANPGSASAPLLEVEHLVKYFAVREVQGLTMVRNTVKAVDDVT
                     FTLAQGETLGLVGESGCGKSTVSRCLTRLETPTSGAIRYKGRDIATLPEKQLRPLRRE
                     IQMIFQDPYSSLNPRQTVGAILSTPLRVHGLAKGKEKARSQELLELVGLNPEHINRYP
                     SEFSGGQRQRIGIARALAVEPKLIIADEPVSALDVSVQAQVMNLLSRLRSELGLSFIF
                     IAHDLGVIRHFCDRVAVMYLGKIVELGNKQHIYGTPQHPYTQALLSAAPDINVVRGIP
                     PKERIRLVGDPPSPISPPSGCRFRTRCWKAQEICATQEPPLEDKPGGGDDDVPGTGDE
                     HVIACHFPEIKSTLVAEVTR"
     gene            complement(13860..14885)
                     /locus_tag="PLK64_00555"
     CDS             complement(13860..14885)
                     /locus_tag="PLK64_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV78259.1"
                     /translation="MTEAPAGEVVLKITDLCVRFPSDEGPVNAVQDLTYEARLGRTLA
                     IVGESGSGKSVSSMAVLGLHNPKRSTITGSILLDGNEIVGADESLLRRLRSNTAAMIF
                     QDPLSALHPFYKVGSQIAEAYLAHNSVSKDIAAKRAVEMLELVGIPNPARRAKQYPHE
                     FSGGMRQRAMIALGLVNNPKLLIADEPTTALDVTVQAQILDLVKDLQREFGSAVILIT
                     HDLGVVAEVSDDILVMYAGRAVERGLAKEVLARPQHPYTHALLQSLPSASEDSDQLQT
                     IKGSPPSLVHLPSGCSFHPRCMVKDRVPGDPCATLLPELRVSSPGRQIRCHLTNPESV
                     LDQEVVS"
     gene            complement(14960..15946)
                     /locus_tag="PLK64_00560"
     CDS             complement(14960..15946)
                     /locus_tag="PLK64_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV78260.1"
                     /translation="MLIYIARRALMAVSVLLVVLVATFLLFFAGPSDPAQAMCGELRC
                     TPQRLEDIRRSLGTDRPVAEQFTEYFKGLAVGREIYVSGAPRTCSAPCLGFSFRTGIE
                     VKEALATRFPNTAMLALMTMAIFLTVGISTGVLAARRRGTALDRGVVGFSQVFGSIPS
                     YILGLLFALYTTVLYPILPRYANRSDGLGPWFVGLIAPAIILGLIYATGYVRYARASM
                     VDTLNQDYVRTARSKGISEQKVVYRHALRAALSPVVTILGLDMAGLLSGTLVTEHIFG
                     VDGIGKFAIESLRNDDLPIIMGTVLIGCVLVVVMNLIVDIAYSFLDPRVRLS"
     gene            complement(16047..17822)
                     /locus_tag="PLK64_00565"
     CDS             complement(16047..17822)
                     /locus_tag="PLK64_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPV78261.1"
                     /translation="MRWTHVLAVGAVASLGLTAACSAPASTPGATTGATASQTADSKA
                     AAAIDPTAKGPAPEVAGAKKGGTLTVSYSTAPADMDPSAQFYQDSGAIMTRLTQRSLT
                     SFVNRNGKQVLVPDLATDLGQVSADGLTWTFTLKDGIKYSDGSAVVADDISYAVFCFI
                     ENTDTGPTYQVDFLKDGAKYEGPWDNPETENPFGDTFAGVEAQGNKVIFHLEKRWETL
                     PYFATFTQVSPIPKAKETKNRDYGNTALSTGPYMIKSFTQGTELTLVRNPNWDAKTDP
                     ARTAYVDSYAFKFGQDSIKVQQGILASNGPDATTLNWDGIDASLADQVTGAKSSQFIN
                     GQSSCVVAVNMDSRKIPLEVRKAIAVAYPFDDINKAAGATPLSQTPASTLIPPQIPGW
                     LDYKLPGLTGTGNGDPVKAKEMLKAAGKEGFELVYYYTNDDPSNVAQQVNQVRKTKLQ
                     EAGFTVKDLGVPNKERRTLISKLDGPHNMLQSPGGWCFDWPSADSIFPPMVSSTQIKG
                     GGTNWGNLSDAKIDAEMERILKLSIADQGPEWGKFDKWLMETYLPAIPYYYDRSNLLF
                     GTKVKNVINDPNHGMPVLDAIWVEQ"
     gene            complement(17892..18893)
                     /locus_tag="PLK64_00570"
     CDS             complement(17892..18893)
                     /locus_tag="PLK64_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV78262.1"
                     /translation="MGSTTEVADDSAARATAAAATDRRSGRSPSQIAWDRLKRDKVAI
                     TSLVIVIFFILVAIFAPLLVALEGEELNKYHPELISALNTYPIIGANPQHWFGVEPRT
                     GRDLFAVWAFGARPSLIIGFVAATFSTILGIIMGLAAGYLGGKVDKVITWIIDLLLSL
                     PFLLMVLALVPIIQKRFAAPGEILTAEETSSIRFVVLIGILVFFGWIGLARLIRGEVL
                     SLREREFVQAAKVIGVPTRAILFREILPNLVGPIIVSASIAVPAYITAEATLSYLGAG
                     LVEPTPSWGRTVSNAQNAYARYPLFLWPPVLSIALLVLALSLLGEAIRDAFDPNTRR"
     gene            complement(19171..20574)
                     /locus_tag="PLK64_00575"
     CDS             complement(19171..20574)
                     /locus_tag="PLK64_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPV78263.1"
                     /translation="MFGAGAGTGADAAYPALDLPLAVRMRPRSLAEVRGQEAVLRVGS
                     PLRRLVEGSSGPAGPLSAILWGPPGTGKTTLAHLVAGASGRRFVELSAVTAGVKDVRE
                     VMAAATRERDLYGRHTVLFLDEIHRFTKAQQDALLPGVENRTVILVAATTENPSFAVV
                     APLLSRSVIVALVPLSDDDVAAVVGAALDDARGLDGAFTLDPDATAHLVRMAGGDARR
                     SLTALEAAAGVAGDQVPLGAAEPTRDTPTAITLAHVERAMAQAAVRYDRAGDQHYDVA
                     SALIKSMRGSDVDAALHYLARMLEAGEDPRFVARRIVIAASEDVGMADPTALQTAVAA
                     MHAVAQIGMPEARIVLAQAVVHVTLAPKSNAAYLGIDAAIADVRAGRGGPVPLHLRGS
                     GYAGSARVAASAAAAGAGPADAVYRYAHDAPEGVATQQYLPDDLSGRVDYYRPTGRGF
                     EERLATRWTWLKGLLRG"
     gene            20785..22911
                     /locus_tag="PLK64_00580"
     CDS             20785..22911
                     /locus_tag="PLK64_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPV78264.1"
                     /translation="MVLTWAVLAVAAFLVAAAGVGGPSLFSKLSTGEPTAPGEATTGR
                     DLLAAAEPDRPAILLTVDGVDLVSPAVATAAAAATADIAAIDGVADVASPFEFPAGTA
                     DPRAAAFVTAGSTPPKAFLMVTRLEAGLEGAAQDRAEVAVRAAYTRMGGTLPGASIAI
                     GSGAALGDEIVGQMEADLRLGEGIALPLSFALMVVVFGGFVAAGMPIIGAVVSIGGAL
                     AALYGFSTVMDLDAATVNVVTLLGLALSIDYGLLFVSRAREELRALAEGRPAADLTHE
                     QIRLAMARTTATAGRTVLFSGVTVAISLAGLMVFRSPTIRAIGAAGMSVVLVAMLVGV
                     SMMPALCTLGARRLLARGTETAPDEGVFSRLAAAVHRRPWVVMIATSALLLAMAVPAL
                     SMRLTSSGVELVPVGSPQRDFYERLDRDFPALRAPTATVVVNGSLQQAQDWAQGTVAR
                     LPGVTQAQARDLGDGYAAVAVHTGAAPLDEGSRALVETLRANRPAVPIWVTGQAAALL
                     DLTGSIAAGAPYAGLLVVFATFVLLFLMTGSVVIPVKALALNVVSLGASLGALTWIFQ
                     DGHLASALGFTSVSAIESIIPPLVIAFGFGLSMDYELFLISRIAELHQAGVENNRAVE
                     LGLQRSGRIVTSAAALVVIVFAGFIAGKMLIIKEMGVALTVAVLLDATLVRMLLVPAT
                     MELLGERNWWAPAPLARWHARHGIQE"
     gene            complement(22915..24696)
                     /gene="aspS"
                     /locus_tag="PLK64_00585"
     CDS             complement(22915..24696)
                     /gene="aspS"
                     /locus_tag="PLK64_00585"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPV78265.1"
                     /translation="MLRTHEAGTLRADHSGQTVTLAGWVAKRRDHGGVAFLDLRDASG
                     VVQVVARDEVLDDGGGHDLRNEYCVQITGEVRLRPEGNANADLPTGAVEVVASTLEVL
                     NAAAPLPFQIDERVTVGEEARLKYRYLDLRRPGPGAAIRLRSKVNAAAREVLAGHDFV
                     EIETPTMTRSTPEGARDFIVPARLAPGSWYALPQSPQLFKQLLMVAGMERYYQIARCY
                     RDEDFRADRQPEFTQLDIEMSFVEQDDILALGEEIAVALWKLIGVDIPTPLPRLTYAE
                     AMARFGTDKPDTRFGQELVDCTAYFADTAFRVFQADYVGAVVMPGGASQPRKTLDAWQ
                     EFAKQRGARGLAYVLVQEDGELGGPVAKNLSDAERAGLAAHVGAAPGDCVFFAAGVTK
                     ASRALLGAVRLEIAKRGGLIAEGSWSHLWVLDAPLFEPAGDAVAAGDVAVGSGAWTAV
                     HHAFTSPKREFIDTFDSDPGSALAYAYDMVCNGNEIGGGSIRIHRRDIQERVFAVMGL
                     SEEEAREKFHFLLDAFAFGAPPHGGIAFGWDRICALLSGAESIRDVIAFPKSGGGYDP
                     LTGAPAPITPQQRKEAGVDSRPVPPTA"
     assembly_gap    24991..25090
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25091..26553)
                     /locus_tag="PLK64_00590"
     CDS             complement(<25091..26553)
                     /locus_tag="PLK64_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78266.1"
                     /translation="MSGEWQVTHLLNECRSFLRTLGHVAIALVWPPSLIADIERFRRT
                     ISNPPPGDPATIEGMATSLDTAGRAVDEVRADLDRRRVGLPAIVVGVAAGTATQAAAA
                     LSSGLSHEVTGLALSARALRRYAASLQDAITEHGRVDDHFDDAVTHLRRCADLTVEVH
                     GVDTHIPDPVAVTREVSRALADAREGVRTAMRAYEQAETALQALQREGSDGRGWAVLA
                     SAPRVPGFTVLDVAVMVDQWRTSDPDNRLLNPDQWQRVGAMLAALTPAQRAALDELLA
                     AAGSSTQRAVLLSTLAAGADMEQLRFMADAVRSMSDEDIARMMSLNTDNLTSGATYGL
                     DRVIQDPTTGNTCASTSLLALLARSDPMLALWLTSGQRPPGWDPAYLQHLTEAQWNGA
                     TFLERFIAAQHSVLTTTDSDLGTWPEALGTPPWGAAALATLGSPTAYESVLYDDQDPA
                     LVRKLMSRAAEAANNGTPVPFYVGDDYTPRHVMLITG"
     gene            complement(26550..26867)
                     /locus_tag="PLK64_00595"
     CDS             complement(26550..26867)
                     /locus_tag="PLK64_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78267.1"
                     /translation="MTTSTDEALHLARVGDALVDAQVDVTALRQIAWAAELAGLRAVL
                     ALGPGLLTDPRAGAVLVDALQPLAVDLADGQPQSPDLTHRVRRFFADLVMVVGALTHE
                     DPA"
     gene            complement(26864..27133)
                     /locus_tag="PLK64_00600"
     CDS             complement(26864..27133)
                     /locus_tag="PLK64_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78268.1"
                     /translation="MTGSMTWDEGGAGEASGQVASMADAVQAQSRRLAGITVNQGDAT
                     GMIRELLHTWATELDLRGEALDVWATAVRAQTETVARTDHRMRLA"
     gene            complement(27304..27993)
                     /locus_tag="PLK64_00605"
     CDS             complement(27304..27993)
                     /locus_tag="PLK64_00605"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPV78269.1"
                     /translation="MAFDAPIGTAALLRDEIAPKPVVASDVVWQGLVFDVRRDRVDLG
                     AEVVTRDYADHPGAVLILALREIDGVDHIVMIRQYRHACGSYLWELPAGLLDIPGEPP
                     RDAAERELREEVDLVAGRWDLLIDAYASAGVFPEAYRVFLARDLAPAPGEPHVRTGEE
                     ADLTVRWVSLDDAYAAALAGQLHNPGALLGILAAWGARAQGWSTLRPDDAPWPEHPAY
                     RDGHRTGPQAG"
     gene            complement(27995..29695)
                     /locus_tag="PLK64_00610"
     CDS             complement(27995..29695)
                     /locus_tag="PLK64_00610"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPV78270.1"
                     /translation="MAEQTKHIFVTGGVASSLGKGLTASSLGHLLRSRGLRVTMQKLD
                     PYLNVDPGTMNPFQHGEVFVTDDGAETDLDIGHYERFLDVNLEGTANVTTGQIYSTVI
                     AKERRGEYLGDTVQVIPHITNEIKARMRALATGDKDAPDLIITEIGGTVGDIESLPFL
                     EAARQVRQEIGRDNCFFLHVSLVPYLAPSGELKTKPTQHSVAALRQVGIQPDGLVLRA
                     DREIPEPIKRKISLMCDVDQDAVAAAVDAPSIYDIPKVLHGEGLDAYVIRRLGLGFRD
                     VNWTSWNEMLKRVHDPEHQIEIALVGKYIDLPDAYLSVTEALRAGGFHHDAKVVIRWV
                     ASDECQTEAGAQRALGGVDAILVPGGFGVRGIEGKLGALRWARERQVPTLGICLGLQC
                     MVIEYARTVLGIADASSSEFDPQTTHPVVATMAEQLAFVEGAGDLGGTMRLGRYAARL
                     TPGSVVATAYGTTEVSERHRHRYEVNNSYREQLEGAGLVFSGLSPDGSLVEFVELPAD
                     VHPYFVATQAHPEFLSRPNRAHPLFAGLVGAALDRQRAQRLVEVERTTRPESQTAAVG
                     "
     gene            complement(29707..31539)
                     /gene="recN"
                     /locus_tag="PLK64_00615"
     CDS             complement(29707..31539)
                     /gene="recN"
                     /locus_tag="PLK64_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPV78271.1"
                     /translation="MLLEIRIAGLGVIAEATLRLHSGLTVLTGETGAGKTMVVQGLGL
                     LLGSRADPALVRSGRPSLSVEGIVRLPTGHPARERAQDAGGELDEDELVLARTVTAEG
                     RSRAHIGGRSAPVGVLGEIGEHLVAVHGQADQWRLRRPDEHRDVLDGYGGEAVAAALA
                     AYREGYAALTATVTERDTLRGLDRDRTREVDSLTAALEHVARVDPQPGEDEALRAEDT
                     RLAHAEGLRDAAGAAHGLLLGDDGYAADAGEQGAATDLLARARAQLGPVARHDPRLSA
                     LDERLAEAGYLVADIGADLAAYLADLDADPARLAWVQQRRADLAGLTRRYGESIDDVL
                     AWSGEAASRLALLQSADERLAALDARVAALTAEVTAAGERLSAQRRAAADRFANAVGD
                     ELAHLAMGSARIQVAVTPRPLGPHGADDVEIQLTTNSGAPARSVGRAASGGELSRLML
                     AIEVVSVSGSTPAALVPTFVPTFVFDEVDAGVGGKAGLDVGARLAALATHAQVVVVTH
                     LAQVAAFADRHLVVHKSDDGSVTASAVREVEGDERLRELARMMGGGTSEAGLEHARVL
                     LAEATTRRRERHTAATEVAPARRAGAAKTRRGARSPSAVAPDIT"
     gene            complement(31566..32486)
                     /locus_tag="PLK64_00620"
     CDS             complement(31566..32486)
                     /locus_tag="PLK64_00620"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HPV78272.1"
                     /translation="MTRRVLLVPHPRRPDIGDIARDVATRLGAQGVATTMIATDAAAL
                     HATDIGPTTVVPDAGAANNCELVCVLGGDGTILRGAELSRGTDTPLLGINLGKVGFLA
                     EVEMADLATTVDRIVARDYTVEDRMTLDVTVSHGAGVDSRTWALNEVSIEKAARERML
                     EVTVEIDGRPLSTWGCDGVIIATPTGSTAYAFSAGGPVVWPDVEAILLVPNSAHALFA
                     RPVVVGPRSRLAVEVIRDAEGQGVLWCDGRRHVDLAPGARIDVCRSAVPMRLARLSHA
                     PFTDRLVEKFDLSVAGWRGNGAGRPQPPTD"
     gene            complement(32483..>33083)
                     /locus_tag="PLK64_00625"
     CDS             complement(32483..>33083)
                     /locus_tag="PLK64_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HPV78273.1"
                     /translation="GALETFGPQGLTVAGRRALDVGASTGGFTQVLLSQGAAQVTALD
                     VGHGQLVEQVAGDPRVIERSGTNIRHCTAEDLGGPFDLVVADLSFISLRLVLPVLATL
                     IEPSGDLVLLIKPQFEVGRERLGKGGVVRVDADRLAALRGVLDAATDVGLGVRGLVAS
                     PIRGTTGNREYLGWFTSRAHDRAEAADLERLVAAMESPS"
BASE COUNT         4920 a        11304 c        11738 g         5021 t
ORIGIN      
        1 tgagccggac cgtctcgaag gtggtcttct tcgaccgcgg gtgcggcgag aagatgacga
       61 cgtcgcgcgc cttgatggca tagatcgcgt tgcccgccgg ggtgaggtcg gggttggtcg
      121 tcgggacgat gcagccgatg atgccggccg gcttgccgta cttcaccagg cccttctcgg
      181 ggatctcctc gatgatgccg acgctcttct ggcgcagggc atcacgcagc acgccccgga
      241 tcttcatccg cttgcccatc cggctcaccg ggtcgccgag gccggactcc tcgatgcctt
      301 cggccaccaa ccgggtgaag gtcaccttgt tggacacggc ccaggcgacc gcctggcaga
      361 gccggtcgac gcgcgcctgg tcgtaggtct cgatgatggc gagcgcggcg cgggcgcggg
      421 cgaagatctc gtccagttcg gcctgctgat cggcggtgag ttccttcttg tcagccatgg
      481 tgatgcctcc ggcgcggggt gttcggggaa acggacggct acgggatgtc ccggacgagc
      541 tatggggtat gccgtccggc tgggtccctg ggcgtccctg cgttggagcg gctccccttc
      601 agtgtggagg acccaggaga tttatgcact atctagttag ttaccaataa tgtgatctgt
      661 gtcacatgcc tcgtccggtt agggtcgagc ccatgtcgca ccagtcgtcc gctgccctgc
      721 tggcgctgca tgccgtccga gtgaccggca tggcatccac cgagcgggcc gccgcgcgtt
      781 acggactccc ccaggccgag gcgcacgagt tgctcgagga cgaccgggcc cgcggttatg
      841 tccgccgggt cgagttcgtg gatctggcgg gatggaccct caccgagtcc gggcgcaccg
      901 aggacgagcg ccgcctggcc gccgagctcg cgctgactgg ggcccgcccg gtggtgcagg
      961 ccggcgccga cgacttcgtc ggtctcaacg cgcgactgct cacggcgatc accgactggc
     1021 agttgcggcc ccaaccgtgg gaccggctgg ccgcgaatga ccacaccgac catcgctggg
     1081 atgaccgggt gctgcgtgag ctcacccgga tcggcgagct cctcgccccg gtctgcgacc
     1141 cgctggcagc ggccctgagc cgattcgagg gttacccaca gcggtatgcc gccgcgatgg
     1201 cccgagtggc cgacggcgag cgctcctggg tggatcgggt cggaccggac tcggcccatg
     1261 gggtctggct ggaactgcac gaggacctgc gtgccaccct cggggccggg ccgtgcgtca
     1321 cgccgtcttg agctcctgca gggcgaaggt catcgccagg ttgtaaccga gcgtgccgaa
     1381 gccggccatc accccgacgg cgatgtccga gaggtaggag tggtggcgga acggctcccg
     1441 cttgtggacg ttcgagatgt gcacctcggc gaacggcacc tcggcggcgg cgaacgcgtc
     1501 gcggatcgcc acgctcgtgt gggtgaaagc gcccgggttg atgatgatga acgccgtccc
     1561 gtcggtgcgg gcggcgtgca gccgctcgac gatggcgccc tcgtagttgc tctggaagca
     1621 ctcgacgctc accccggcgg cctcaccttg agcccgcacc gcggcctcga cgtcggccag
     1681 cgtcgtgctg ccgtagatct cgggctggcg agtcccgagc atgttgaggt tcgggccgtt
     1741 gatgagcagg accgacatgg cgttctcctc ggtgtcgtgc gttggcgggg tctacagatc
     1801 gggtcgggtg cggggccgcc ttcaggaggc gagctcggcg aaggcggctt ccagcagtgc
     1861 ttcgtcgggc ccttcgaggc gcccaggcgt ggccaggtca tcgagcacga cgaaccgcag
     1921 cagcgctccc cggctcttct tatcgcggcg catcgccgtg agcaaccgcg gccaggcctg
     1981 cccgcctgcg ctcgggtagg tcaccggcag cccgagcgag gtgaggatct cggtatgccg
     2041 tgccgccacg tcagcgggcg tccgcccggc caggcgaccc agcgccgcgg catacaccag
     2101 cccgaccgac accgcgttgc cgtgccgcca cgagtagccc tcgacctgct cgaccgcgtg
     2161 cgcgaaggtg tgcccgtagt tgaggatctc gcgcagcgac gactcgcgca ggtcggcggc
     2221 gacgacggcg gccttgaccc ggatcgagcg ctccaccaac tcggccagga cgtcgccggc
     2281 gggatccagc gcggcagcgg ggtccgcctc gatgagctcg agcgtgcggg ggtcagcgat
     2341 gaaaccgtgc ttgacgacct cgcccattcc cgcgacgagt tcggcgcgcg gcagggtgtt
     2401 caaggtggcg atgtcgcaga ccaccccggc cggggggtgg aaggagccga cgaggttctt
     2461 gccctcggcg gtgttgatgc cggtcttgcc gcccaccgcg gcatcgacca tcccgacgag
     2521 cgaggtcggc acctggacga cagcgacccc ccgcagccag gtggccgcga taaagcccgc
     2581 cacgtcggtc acagctcccc cgccgacccc gatgaccgca tcggagcggg tgaacccggc
     2641 ctgaccgagc accccccagg cctgcgcggc gacgatgggc gtcttggccg cctccgcgtc
     2701 ggggacctcg tgcaggtatg ccgtccgccc accggcctcg atccgcgcgg cgagcagagc
     2761 ggccaggtcg cgcccagtcg gggcgtggac gacaagcacc ttcgcgaccc agtcggccag
     2821 caggtcgacg gcgaaggtgg cgatcccccg gccgacgaga acgtcatagt cgtcgccgac
     2881 accgacgcgg acgagatcag ccatgctcgg acgccccgac ggccagatcg tccagcgcgc
     2941 cggcgagttg ctcgacgtcc ggtgacaccc cggcttgggc ccggccaagg gcgtccagca
     3001 ggacgaaccg ccaacccttg cggtatttct tgtcgcgcgc gagcgcatcg acgaggtcgt
     3061 ccaaggagat ccgggcgctc gtgggcagac ccagggccgc caacgtgtcg cggtgggcgg
     3121 caacgccgtc ggcgtccagg cggcccaagc ggtgggacag gtgagcggcc gccatcaacc
     3181 ccagggccag ggcgccgtcg acaacctgcg ggtcaggcac ccgcgcgaac gcccgtgaga
     3241 aggtgtgtcc gtagttgagg tggactcgcc caccctgctc acgttcgtcg gccgtgacca
     3301 cggccgcctt gatctcgaca ctgcgccgca cgatctcgac gagggtctga ccgtcgcgag
     3361 cgacgatcgc gtcccggcgc tcgcggagcg tgtcgagcag ggcgggatct tccaggaggg
     3421 cgtgcttgac gatctcggcc agcccggcgc gatagtcggc atcgccgtag gtcgccgaca
     3481 ggaccgccaa ctcgatgtcg cacacgacga ggacgggctg gtggatcgtg cccaggaggt
     3541 tggcgccgtg gggcaggtcg atcgagcact tgccgccgat cgccgagtcg gcctgtgcct
     3601 caagggtggt cggaaccagg gcgtgtgcca tcccccggtt gtaggtcgcc gcgaggaagc
     3661 cggtgacgtc gcaggtcgcc tccccgccga cgccgacgag caggtctccc cggtgcaccg
     3721 acaactccgc gagcgcggag gcggcgaggc cgaccgcatc gagggtcttg gcagtgctcc
     3781 ccgacgggag ggtcacccga cgcacgggga tcgccagcgt gcgcagttgc gcgacgatct
     3841 cggcagccgc ctcaccctcg tcggccgttg ccaggatgac gatctgctcg gcatcgggca
     3901 tcggcggcag cagcgcggcg atctgcgaac ggatgccgcg accgacgtgg acccgatagg
     3961 acccgccacc gggggccacg aggacccctg ccgtcccgtc gagggcagcc gtgccggtga
     4021 tcgcgtcgat gatctcgagc ccgatgtcct cgacccgccg gccggtcgtg cgcgaggtga
     4081 tggtggccgt cgccgcgtag atgtccaggc gggcggcata cagggcgggc aggccggcgt
     4141 cgtgcaggag cgggcggtat tcgtcccgcc cgacccgagc cagggcctcg tccaactcga
     4201 cgtgcagata gatgaccgtg tgaccacgca gccgctcccg agtctcggct cgcccacagg
     4261 cgccgccgcc gagggacaac acgcaggcag gtccgtcgag caactcggcg atggtggccg
     4321 cttcgacgtc gcggaagccc gactccccct ccgcggcgaa gatgtccttg atcgagcgcc
     4381 gctgctggtg ctcgatgacc aggtcactgt cgacgaacgg cagcccgagg cgttcagcga
     4441 tgaggcggcc gaccgtggtc ttgcccgctc ccatgaatcc gacgacgacg atcaccgcac
     4501 gagcctcctc accaggtccg catccgctcg gggacggccg cgaggtatgc cgcgtggttg
     4561 cgtgccgtct cggcgacaga gtcaccgccg aacttctcca cgaccgcttc ggcgagcacg
     4621 agggcgacca tcgcctcggc gacgactccc gccgctggga ccgcgcagac atcggatcgt
     4681 tggtggatgg ccttggccgg ctcgtcggag gccacgtcga tggtgtcaag cgagcgcggg
     4741 acagtcgaga tcggcttcat cgcggcgcgg acccgcagga tctggccggt cgacatgccg
     4801 ccctcggtgc cccctgcccg accggtgcgc cgctcgatga caccgtcgtc gcggcgggcc
     4861 atctcgtcgt gggcggccga gccgcgtcgt ttggccgtct cgaagccgtc tccgacctcg
     4921 acacccttga tcgcctggat gcccatgagg gcccccgcca gccgcgcatc gaggcgccgg
     4981 tcccagtgga cgtgggagcc caggcccggc ggcacgccgt aggcgagtac ctcgacgacc
     5041 ccaccgaggg tgtcaccgtc gcgccgggcc gcctcgacct cggcgaccat ggccgccgaa
     5101 ccttcggcgt cgagggtgcg gaccgggtcg gcgtcgaggc ggtcgacgtc gtcgggatgg
     5161 ggcaacgcgg ccgtgtctgc gaccgcggcc gtgccgatgg cgacggtgtg cgacaccaac
     5221 cggatcccga ccgtctgctc aaggaacctc gcggcgacct cgcccaacgc gacccgtgcc
     5281 gcggtctccc tggccgaggc gcgttcgagg atcggacggg catcgtcgaa gccgtacttc
     5341 tgcatgccga cgagatcggc atggccgggg cgcggccgag tgagcggccg attgcgagcg
     5401 atctccttct cggcgttgac gtcgttcgcg gcggcatacg cgtcggcatc gaccggctcg
     5461 gggctcatca ccgtctgcca tttgggccac tcggagttgc cgacccggac ggcgatcggc
     5521 gagccgaggg tgaacccgtg ccggacgccg ccgagaaact ccacgtcgtc ctgctcgaag
     5581 gccatccggg cccctcgccc gaaccccaga cggcgccggg ccagagcccg gcccaggtcg
     5641 gcacgggaca ccgacacccc cgcgggcagg ccttcgagga tggcgatgag ggcgggcccg
     5701 tgggactccc cggcggtgag ccaacgcagc atgctggcat cctcccacgc agcctgggcc
     5761 accggcccga cgacccggat ggcaagacgt cacccgagca ggacagccac cagcgccccg
     5821 acgagcagat acggcccgaa ggccaggtcc gtgcgcatgg tgagccgccg ggtcgccaac
     5881 cccaccaccg agaccaggcc gctgatgacg aacgcgagca tcaaccccca ccacggcagg
     5941 cgtccgtcga cgctgcccag ggctatcccg aggatgcccc cgaccgtcac gtcaccgagg
     6001 ccgaactggc cccgcgagat gagggcgagg acgagcagga cgaggtatgc cgccacgccg
     6061 ctgacgagcg cgcggacggg gagcgtccaa ctcccggaca cggcgccggc gaccaggacg
     6121 atccccagca cgcccgcacc cagcggatag gtcagcccgt gcgggagccg gtggacgtcg
     6181 atatcgatcc acgacaacgc caccccggcg aacccgacaa gcaacaaggc cggcagcaag
     6241 gtcagggtgc ctggccctcc gacccgccac gcgagcaggc cccagacgag gggcacgagg
     6301 gcgacgagca gccacggtcg gcgcagcacc ggccggtccc attcgtcgtc caggcgatag
     6361 ccgccggtaa ccaactcccg acgcagccag ccgccgaggg cagacccgac caccacagcc
     6421 gcgaggacga gccaccaggg gccggtctgg gtgaagacgg tcattggagg tcccctggct
     6481 gcggtgccat cacacgtcgg cggcggccag cagggccgcc cgcatcgcct cggtcggagc
     6541 atcgcgggag gtcatcagtc gcacctgctc tcgcgcctgg tgcagcaaca tgtcggtccc
     6601 gccgacgacg gcatacccga tctcctgggc gaccccgagc agcgggctcg ggccgtcgcc
     6661 gtagacgcag tccaggaccg ccgacccggg gtgggcctcc ggcagggcac cgagcagacc
     6721 ggcatacgcc gtcgcgggga tggtgctgac gatgacgtcc gtgccgtagg gccaatgccc
     6781 catcggcacg gtcacggcat cgagacccag atcgcgagcg aacgcgaggg tgccgggacg
     6841 cacggcgtcc cggaccatga agctcacctg cgccgcaccg aggtcggcca gcgcagccaa
     6901 ggccgagcga gcggtggcgc ccgctccgac gaggacggcc gaacggacgt gccccacgcc
     6961 ggcttcccgc agcgcctcga cgatgccgta gacgtcggtg ttgtgccccg accagcccgc
     7021 ctcgtcacgc accaacgtgt tgatggcacc cacctggacg gccaggtccg acgccgtatg
     7081 cacgacgtcg aaggcggcct ccttgagcgg catggtcagc gacagcccgc gccaggtctc
     7141 gtcgagggcg gccacgtggg tcgccaactc gtcggcctcg acccggatcc ggtcgtagct
     7201 ccaatcgccg agccccaacg ctcggtatgc cgcgcggtgc agcaccggtg acagggagtg
     7261 ttcgatgggc gaaccccgaa ccccgcatcg gttgtgcacc acagacctca tcgagagcat
     7321 ttcccctggt tctccgccgc gttgcaccag gcttggaact gcgcaacgta ggtgtcatgc
     7381 tcggcctggg tggcggcgaa cttggtctcg cccgtctcgg ggttgaccgc gacgaagaag
     7441 agccagggtc cctcggccgg cgccactgca gcctggatcg aggcgagccc ggggttggcg
     7501 atcgggctgt ccggcaggcc gtccttggtg taggtgttcc acccgttgac gctggcgcgt
     7561 tcttcgtcgg tggtcgtgat ggcccgcttc ttgacgccgt agctcaccgt cgagtccatc
     7621 tgcaggcgca tcggcttggc cagccggttg agcacgaccc gggcgacctt ggcccggtcc
     7681 tcggaccgct tggcctcagc ctcgacgatg ctggcgatga tcatgatccg ctcggcctga
     7741 tcgggttgga tctgcaggtc ggtaaggacc ttgacggtct tgccgaccat ggcggtgagt
     7801 tgggtgacgg cggagtcctt gtcggagaac tcgtaggtcg cggggaacag gtagccctcg
     7861 acgtttccgc cggccgacgg gggcagcagc gccaacacgt cggggctctt ggccgcagcc
     7921 tcgtagtcgg ccaccgaatg cttcgtggcc ttgcccaaca aggcatagat ctcgctcgcc
     7981 cagagcccct cacgcacggt gaccttgggg ttctgtcggt tcttggggtc gacgaggatc
     8041 gccaacgcgt cggcggcctt catctccttc ttcagggcgt agtcgcccgg ttggatcccg
     8101 gcgcaccgcg agtcggcgct gcacgcgtcg acgaacgcct tggcggtaag gacgatgccc
     8161 tgggtctgca ggttggatcc gatcgcgcgg cccgtatcgc cgtcgttcac cgtaaaccgg
     8221 gccgccgcac cccccggacc ggcaaagtct gacgcgtccg cggagcggtc gaacaacggg
     8281 cgcagcgcgg tgaaaccgag gaatccggca ccggcgatga gccccagcgc cacgaagagg
     8341 atgaggaacc ggcgcacccc acgaccggtg ttgcgccgct cccgcctggc ccggcgtgag
     8401 tcttcggcga acccgaaggc cgctgcatcg ggatcgtggt agccgccgtg cccctcctcc
     8461 acaccgtcgt gctcgtactc gtgctcgtac gcgtactccc cctcgtgcgc gtcgttcccc
     8521 gcgtgggcgt actcgccttc gtggacatcg ggcccttcgt gggcgtaccc accggcgccg
     8581 gaatagtcga cctcgtgagc gtcatagccc tcatgtccgg cgtgcacctc gtgctcgccg
     8641 gcgacctccc ccgcggcctg gccccagacg tcgtcgccgc ggggatcccg cagcgggcgc
     8701 tcggtcatgc tcgtcccttc gtccggggct tacgcccgcc tgtgctgacc agctcccccg
     8761 gttgagaccc ggtagcccgc tctgcctcga gtgctgactt gaggatgagc actgcggcca
     8821 cctggtcgac gacggctcgg tgcgtgcgtt ccgcgcggcc actgtcgtgc aacagccggt
     8881 gggacgcgac gctggtgagt cgctcgtcga ccaaccgtac gcggacaggg gccacgagcg
     8941 cagcaagctc cgcggcatac gccctggctt gtcgcgccga ttccccggaa accccggaga
     9001 gttgcagcgg caaaccgacg tagacgcacg tcgcgtcgcg gtcgcggacc aacgccgcaa
     9061 gcgcggccag atcggcgtgt tcggggctct cgtcgcgggc cagcgtcgtc accggagtgg
     9121 cgagtatgcc gtcggggtcg ctctcggcta cccccacccg gacggacccg acgtcgacgc
     9181 cgacccgtcg gccctgcgac ggcatacccc gttaaccctg ggcccgaagc gagtcctcga
     9241 cggcgccgag cgaggcaccc atggaggcgg cgtcgacgcc gccgccctgg gcgaggtcgt
     9301 ccttgccgcc accgccaccg ccgagcacct gggcggccag gcggaccagc gccccggcct
     9361 tgactccgcc ctggcgagcc ggtgcgttgg tcgcgacgat gacgacgggc ttgccgccca
     9421 tggacccggt catggccacg acggtcgcgt gggcatcgcc gagccggctg cgcacgtcga
     9481 gcaccagcga gcgcagatcg tcgggagcga tgccttcacc cgcgtcatgg gtcaccagtc
     9541 ggaccgaccc gatctcccgt gccgacgaga cgagccccgg agcgaccgcg agagcctgct
     9601 gctgacgcgc ggcggcgatc tcgcgttcgg cgtcgcgcag cttggcaagg atgccgctga
     9661 tccgctcggg caactcctcc ggccgggcct tgagcgcctc ggtgagttgg ccgacgatgg
     9721 cgtgctcacg ggcgaggaag gagtaggcgt cggcgccgac gagcgcctcg actcgccgca
     9781 cccctgagcc gatcgacgcc tcgccgagca gcttgacgac gcccaactgg ctggttcgag
     9841 gggtgtgcgt gccaccgcac agctcgcggg cccagtcgcc gaccgacacg acgcggacgg
     9901 cgttgccgta cttctcgccg aacagggcca tcgcgccgat ctcgcgcgcc tgatcctggg
     9961 tcatcacctc ggccgagacc gggaggtcat cgagcaggac ggcgttgacc cgggcctcga
    10021 tctcctgcaa ctgccccaag gggatggcgt gttgagcatt gaagtcgaag cggaaccgcc
    10081 cgggtccgtt ctccgaccca gcctgcgtgg cggtgtcgcc cagggcctca cggatcgctt
    10141 tgtgaaccat gtgggtcgcc gtgtgggcac gcgagatcga gcggcgccgc tcgacgtcga
    10201 cctccgcctg cgcctgttca ccgacggtga cctcgccggc gatgatcgtc gcgcggtgga
    10261 ccgacagccc ggtgatcggg ctctgcacgt cgcggacctg cacgatcgcg ccgttggcca
    10321 actcgatccg gccaccgtcg gccaactgcc cgccaccctc ggcatagaac ggggtgcggt
    10381 ccaggaccag ctcgatgtcc tccccggcga tggcctggcc gacgctgcgc ccgtcgcgga
    10441 cgatcccggc gacgcgggcc tccgacacga cctcgtcgta gccggtgaac tccacacccc
    10501 gcccgagccg gtcggcgacc tcgcggtaga ccccggtgtc gccgtgggcg ctcttcttgg
    10561 ccttggcgtc ggccttggcc cggtcgcgct gctccttcat cagccggcgg aagccggcct
    10621 cgtcgacctg cacgccttgc tcggccgcca tctccagggt gaggtcgatg gggaagccat
    10681 aggtgtcgtg cagcgcgaag gcctcgtcgc ccggcagggt caggacctcg cccccggtgc
    10741 ccttgagccg ggcgaccgcg ccgtcgagca gcgtggtgcc cgaggcgagg gtgcgacgga
    10801 aggcttcttc ctcggcatac gcgatcgtgc tgatccgacc gaagtcggtg cgtagctcgg
    10861 ggtaggactt gctcatccgc tccatgctca ccggaagcag gtgcggcagc gccgggtcgt
    10921 cgtagccaag cagccgcatc gagcggacgg ctcggcgcag catccggcgc aggacgtagc
    10981 cgcggccctc gttgccgggg gtgacgccgt caccgatgag catgaggctg gagcggacgt
    11041 ggtcggcgac gacccgcaac cgcacgtcgt cggggtggct gtgcccggcg tcgcggccag
    11101 agttggcgcc gtagatcttg ccggtgagct cggccgcctt gtcgaggacc gggtagacct
    11161 cgtcgatctc gtagaggttg tccacgccct gcaggatcgc ggccatccgc tccaggccca
    11221 tgccggtgtc gacgttcttg gccggcaggt caccgaggat ctcgtaaccc tccttggccc
    11281 cgccggtgcc ccggatgttc tgcatgaaca cgaggttcca gacctcgagg tagcggtcct
    11341 cgtcgacggc cgggccgccc tcgcggccat agtcgggccc gcggtcgtag tagatctcac
    11401 tgcaggggcc acacggcccg ggaacaccca tggaccagaa gttgtcggcc atgccgcgct
    11461 tgacgatccg ctcgcgcggg atccccgtgt gcttgaccca caggtcggcg gcctcgtcgt
    11521 cgtccaggta gaccgtcgcc cagagccggt cgccgtcgag gccgtagccc ccctgctgct
    11581 gcggggtggt gagcaactcc caggcggcct tgatcgcgcc cttcttgaag tagtcgccga
    11641 aggagaagtt gccgttcatc tggaagaacg tgccgtgccg agaggtcttg ccgacctcct
    11701 cgatgtcacc ggtgcgcaca cacttctgga cgctcgtggc gcggtcgaac ggcggggtct
    11761 cctggcccag gaagtagggc ttgaacggca ccatcccggc gttgacgaac agcaagttcg
    11821 ggtcgtcgta gagcaacggg gcggacggga cgaccgtgtg cccccgccct tcgaagaagg
    11881 cgaggaatcg gcgcctgatc tcggcggtct ccatggtggg tccttgcgtt cgggtcaccg
    11941 tatgccgggt gctcaccggc gcggtgaagg tgtggttcgg gggcgggcag cgtcggggtg
    12001 gccacgcggt caggctgggg gcgggctacc cctgacgagc tcgccagccg gccgggtcgt
    12061 cgagcaggtc gcgggtggcc tgcgggtcca ggcgggtccc cgacggcggg gaatccagcc
    12121 ccagggccag gcgcagttgt tcttcacgct cggtcatgcc ggcccggatg tcagccacca
    12181 cggctccggc ctcggcgagg gccgcagcga ggacgtccgg tggcccgccc tccgtggaat
    12241 accgatcggc caggagccga taggcgtaga cggcaccgac cccgacaagg gcgccggtgg
    12301 ccaggaccag cagtttgcgg ctcattgctc gtgcctgcct ttgcgggtcg agccgccgga
    12361 ggctgcggtg gtcgcgcggc gttggccgac ggccgagcgg acgccgtagc tgaaggcagc
    12421 aaccttcacc agcgggctcc cgagcgtggc ggcgaacagc gaggtgaggg cgttgacgtt
    12481 gctggacatg ctcgcgatgt tggtggtcac gacgtcgatc cgggccaact gttcgttggt
    12541 ggtcgacacg gtcgtcgtca cctcgccgag cagcggaaca ctgccgtcgg ccaacccttt
    12601 gaccccgatg cgcgtctcgt cgaggatctc gcccgccttg ccgacgaccg atcccaaccg
    12661 ccacacgagg taggcgaagg cgagcgcggc aatgacaccg gcgatgtcgc cgatggtcac
    12721 gggcgcgata ctcaccggtt caccctagcc gaacggcata ccgggctgcg cgcggttgac
    12781 gcgcggccgg cgggcggctc agcgcgtgac ctcggcgacc agggtgctct tgatctccgg
    12841 gaagtgacag gcgatgacgt gctcgtcgcc tgtgccgggc acgtcgtcgt cgcccccgcc
    12901 gggcttgtcc tccaacggcg gctcctgggt ggcacagatc tcctgcgcct tccagcagcg
    12961 cgtgcggaac cggcaaccac tgggcggact gatcgggctc ggcgggtcac cgaccagccg
    13021 gatccgctcc ttggggggta tgccgcgcac gacgttgatg tcgggggcgg cagagagcag
    13081 cgcctgcgtg taggggtgct gcggggtgcc gtagatgtgt tgcttgttgc ccaactcgac
    13141 gatcttgccg aggtacatca ccgcgacccg gtcacagaag tgccggatga ccccgaggtc
    13201 gtgggcgatg aagatgaacg acaacccgag ctcggagcgc agccgggaca acaggttcat
    13261 cacctgggcc tgcaccgaca cgtccagagc cgagacgggc tcgtcggcga tgatgagctt
    13321 gggctcgacc gccagcgcgc gggcgatgcc aatccgctgg cgctggccac cggagaactc
    13381 cgacgggtag cggttgatgt gctcggggtt gaggccgacc aactcgagga gttcctgcga
    13441 gcgagccttc tccttcccct tggccaggcc gtggacccgc agcggcgtgc tgaggatcgc
    13501 gccgaccgtc tggcgggggt tgagcgagct gtaggggtcc tggaagatca tctggatctc
    13561 gcggcgaagc ggacgcagct gcttctcggg gagcgtggcg atgtctcggc ccttgtaacg
    13621 gatcgcgccg gaggtcggcg tctccagccg ggtgaggcag cgggagacgg tggacttccc
    13681 gcagcccgac tccccgacca gcccgagagt ctcgccctga gcgagggtga aggtgacgtc
    13741 gtcgacggcc ttgacggtgt tacggaccat cgtcaggcct tgcacctcac ggactgcgaa
    13801 gtacttgacg aggtgttcga cctcgaggag cggcgccgac gcggagcccg ggttggcggt
    13861 catgacacca cttcctggtc gagcacggac tccggattgg tcaggtggca gcggatctgg
    13921 cgacccgggc tgctgacccg cagctccggc aggagcgtcg cgcaggggtc gccgggcact
    13981 cggtctttca ccatgcaccg cgggtggaac gaacacccgg acggaaggtg caccaggctg
    14041 ggcgggctgc ccttgatggt ctgcagttgg tcgctgtcct cggaggccga cggcaggctc
    14101 tgcaggagcg cgtgggtgta ggggtgctgg gggcgggcca gcacctcctt ggccaggcca
    14161 cgctcgacgg cgcgaccggc atacatgacg aggatgtcat cggacacctc ggccacgacg
    14221 ccgaggtcgt gggtgatgag gatgaccgcc gacccgaact cgcgctggag gtccttgacc
    14281 aggtcgagga tctgggcctg aacggtgacg tcgagggcag tcgtgggctc gtcagcgatg
    14341 agcaacttgg ggttgttgac caacccgagg gcgatcatcg cccgctggcg catcccgccg
    14401 gagaactcgt gcggatactg cttggcccgc cgggccgggt tggggatgcc caccaactcg
    14461 agcatctcga cggcgcgctt ggcggcgatg tccttggaga cgctgttgtg cgcgaggtag
    14521 gcctcggcga tctggctgcc gaccttgtag aacgggtgca gagccgagag cgggtcctgg
    14581 aagatcatgg ccgcggtgtt gctgcgcagc cgccgcagca acgactcgtc ggcaccgacg
    14641 atctcgttgc cgtcgagcag gatgctgccg gtgatcgtgc tgcgcttggg gttgtgcagg
    14701 ccaaggaccg ccatcgacga gaccgacttg ccggagccgg actcaccgac gattgccagg
    14761 gtgcgcccca gccgagcctc gtaggtgagg tcctggacgg cgttgaccgg gccttcgtcg
    14821 gaggggaagc gcacgcagag gtcggtgatc ttcaggacca cctcaccggc gggtgcctcg
    14881 gtcacgacgt tgcgctcggg ggcggacgcg gcggcgacgt cgtgtgccgc tgaatcctgc
    14941 gggggcacgg gctgagcgct cacgaaagcc tcacacgcgg gtcgaggaag gagtaggcga
    15001 tgtcgacgat gaggttcatg acgacgacga gcacgcagcc gatgaggacg gtgcccatga
    15061 tgatgggcag gtcgtcattg cgcagcgact cgatagcgaa cttgccgatg ccatccacgc
    15121 cgaagatgtg ctcggtgacg agcgtcccgg acagcaggcc ggccatgtcc aggccgagga
    15181 tcgtcacgac ggggctgagc gcagcacgca aggcgtgtcg atagacgacc ttctgctcgc
    15241 tgatgccctt cgaccgggcc gtgcggacgt agtcctggtt gagggtgtcc accatcgagg
    15301 ccctggcgta gcggacgtag ccggtggcat agatgaggcc gaggatgatc gccggggcga
    15361 tcaggccgac gaaccacggg cccaggccgt cggaccggtt ggcgtagcgc ggcaggatcg
    15421 ggtagagcac ggtggtgtag agggcgaaca acagaccgag gatgtaggaa gggatcgagc
    15481 cgaaaacctg gctgaacccg accaccccgc ggtccaacgc ggtgcctcgt cgtcgagcgg
    15541 cgagaacgcc agtgctgatc cccacggtga ggaagatggc catcgtcatc aacgcgagca
    15601 ttgccgtgtt ggggaacctg gtggcgaggg cctctttgac ttcgataccg gtgcggaacg
    15661 agaagcccaa gcacggcgcc gaacacgtcc tcggtgcccc ggacacatag atctcgcggc
    15721 cgacagccag gcccttgaag tactcggtga actgctcggc gaccgggcgg tcggttccga
    15781 ggcttcgtcg gatgtcctcc agtcgctgcg gggtgcaacg caactcgcca cacatcgcct
    15841 gcgccgggtc cgagggaccg gcgaagaaca gcaggaacgt ggcgacgagt acgacgagga
    15901 ggacgctcac ggccatcagg gcacgccgag cgatgtagat cagcacgtgg acctcgcacg
    15961 agtggatggg cggtgtgcgg ggcgtggggt ggtccccacg ccccgcacac acgggttgct
    16021 atcagtcact gagcattgcg ctcagctcac tgctccaccc agatggcgtc caggaccggc
    16081 atgccgtggt tggggtcgtt gatgacgttc ttgaccttgg tgccgaacag gaggttcgag
    16141 cggtcgtagt agtacgggat cgccggcagg taggtctcca tgagccactt gtcgaacttg
    16201 ccccactccg ggccctggtc ggcgatcgac agcttgagaa tgcgctccat ctcggcgtcg
    16261 atcttcgcgt cggacaggtt gccccagtta gtgccgccac ccttgatctg cgtggaggac
    16321 accatgggcg ggaagatcga gtcggccgac ggccagtcga agcaccatcc accaggggac
    16381 tggagcatgt tgtgcgggcc atcgagcttg gagatgaggg tacgacgctc cttgttcgga
    16441 acaccgaggt ccttcaccgt gaagccggct tcctgcagct tggtcttgcg gacctggttg
    16501 acctgctggg caacgttgct cgggtcgtcg ttggtgtagt agtacaccag ctcgaagccc
    16561 tccttgccgg cagccttgag catctccttg gccttcaccg ggtcaccatt gcccgtgccg
    16621 gtcagaccgg ggagcttgta gtccaaccag cccgggatct gcggcgggat gagcgtgctg
    16681 gcgggcgtct gcgacagcgg tgtggcgccg gcagccttgt tgatgtcgtc gaaggggtag
    16741 gccacagcga tggccttgcg aacctcgagc gggatcttgc ggctgtccat gttgaccgcg
    16801 acgacacacg aggactggcc gttgatgaac tggctggact tggcgccggt gacctggtcg
    16861 gccagcgacg cgtcgatgcc gtcccagttg agggtcgtcg cgtccgggcc attgctggcg
    16921 aggatgccct gctgcacctt gatggagtcc tggccgaact tgaacgcgta gctgtcgacg
    16981 taggccgtac gcgccgggtc ggtcttcgcg tcccagttgg ggttgcgcac gagggtgagc
    17041 tcggtgccct gggtgaacga cttgatcatg tagggaccgg tcgacagggc ggtgttgccg
    17101 tagtcgcggt tcttggtctc cttggccttg gggatcggcg agacctgggt gaaggtggcg
    17161 aagtagggca gggtctccca gcgcttctcg aggtggaaga tgaccttgtt gccctgggcc
    17221 tcgacgccgg cgaacgtgtc accgaagggg ttctcagtct ccgggttgtc ccagggacct
    17281 tcgtacttgg caccgtcctt gaggaagtcg acctggtagg tcgggcccgt gtcggtgttc
    17341 tctatgaagc agaacacggc atacgagatg tcgtcggcaa cgaccgcaga gccgtcggag
    17401 tacttgatgc cgtccttgag cgtgaaggtc caggtgaggc cgtcagcgga cacctggccg
    17461 aggtcggtgg cgaggtccgg aacgaggacc tgcttgccat tgcggttgac gaaggacgtc
    17521 agcgagcgct gggtcagccg ggtcatgatc gccccggagt cctggtagaa ctgggcgctc
    17581 gggtccatgt ccgccggagc ggtggagtac gaaacggtga gcgtgccacc cttcttggct
    17641 ccggcaacct ccggggcggg ccccttggcc gtcgggtcga tcgcggcggc agccttggaa
    17701 tcggccgtct gcgacgcggt cgcacccgtc gtcgccccag gggtcgacgc gggggcggag
    17761 caggccgccg tcaggcccaa ggacgcgacc gcccccacgg ccaggacgtg agtccagcgc
    17821 atgtgttttc tcctttgatg ggatgcctcg agaggttcct caaggctggt tcgacagcag
    17881 cgtggttgac gctaccgacg ggtgttgggg tcgaacgcat cgcggatcgc ttcgccgagc
    17941 agggacaggg cgaggacgag cagggcgatg gacaggaccg gtggccacag gaacaagggg
    18001 tagcgcgcat aggcgttctg cgcgttggag accgtgcggc cccacgacgg tgtcggctcg
    18061 acgagcccgg caccgagata ggacaaggtg gcctcggccg tgatgtaggc cggcacggcg
    18121 atggacgccg aaacgatgat cgggcccacc aggttgggca ggatctcgcg gaagaggatc
    18181 gcgcgagtag gcaccccgat gaccttggcg gcctggacga actcgcgttc gcgcagcgac
    18241 aggacttcac cgcggatgag gcgggccagg ccgatccagc cgaagaacac gaggatgccg
    18301 atgagcacga cgaaacggat gctcgaggtc tcctcggcgg tgaggatctc accgggggca
    18361 gcgaaccgct tctggatgat cgggaccagt gccagcacca tgagcaggaa cggcaggctg
    18421 agcagcaggt cgatgatcca cgtgatcacc ttgtcgacct tgccgccgag gtagccggcg
    18481 gccagaccca tgatgatgcc gagaatggtc gagaaggtcg cggcgacgaa cccgatgatc
    18541 aacgaggggc gagccccgaa ggcccacacc gcgaacaggt cacgcccagt gcgcggctcg
    18601 acgccgaacc agtgctgcgg gttggcgccg atgatgggat aggtgttgag ggccgagatg
    18661 agctccgggt ggtacttgtt gagctcttcg ccctccaacg ccaccagcag gggcgcgaag
    18721 atcgcgacca ggatgaagaa gatgacgatc accaacgagg tgatggcgac cttgtcccgc
    18781 ttgagccgat cccaggcgat ctgcgacggc gaccggcctg agcgcctgtc ggtcgctgcc
    18841 gcggcggtcg cccgagcggc cgaatcgtcc gctacttcgg tcgtcgaacc catcgtcaag
    18901 gcgagcgctc gcttccttcg gtcgtcgatc gtgcgggttg gccaacggtc cctggcgcgc
    18961 accccccaca ggtcgctgcc agtcgccacc gttgtggcta ctaccgcgac accatgtcac
    19021 acaaaaccac cacggggcac gacgctgcgc gtcagcgata tcaaatcgtt acccaccagt
    19081 tctccccgac cgaagacggt tgtccacgca cgacacgacc gaagatctca ccagttggac
    19141 agcgaaacca cccctcggga tggcagccgg tcatccccgc agcaggccct tgagccaggt
    19201 ccatcgagtg gccagtcgct cctcgaatcc gcggccggtc gggcggtagt aatcgacccg
    19261 tccggacagg tcgtccggga ggtactgctg ggtcgccacc ccctccggag cgtcgtgcgc
    19321 ataccggtag acggcgtccg cggggccggc cccggcggcc gcggccgacg ccgcgacccg
    19381 ggcggaaccg gcatacccgc tcccccgcag gtgcaacggc acgggaccgc cgcgcccggc
    19441 ccgcacgtcg gcgatggccg cgtcgatgcc gaggtatgcc gcgttcgact tcggggccaa
    19501 ggtgacgtgc acaacggcct gggccaggac gatccgcgcc tccggcatcc cgatctgcgc
    19561 cacggcatgc atcgccgcga ccgcagtctg cagggccgtc ggatcggcca tccccacgtc
    19621 ttcgctcgca gcgataacga tccgtcgtgc gacgaaccgc gggtcctccc ccgcctccag
    19681 catccgggcc aggtagtgca gggctgcgtc gacgtccgac ccccgcatcg acttgatgag
    19741 cgccgacgcg acgtcatagt gctgatcgcc tgcgcggtcg tagcgcaccg ccgcctgggc
    19801 catggcccgc tcgacgtggg cgagggtgat ggccgtgggc gtatcgcgcg tcggctcggc
    19861 cgcaccgagc ggcacctggt ctccggccac gccggccgcg gcctccagcg cggtcagcga
    19921 acgccgcgcg tcgccgccgg ccatccggac gaggtgtgcg gtggcgtcgg gatcgagggt
    19981 gaaggccccg tccagccctc tggcgtcgtc gagcgccgcg ccgacgacgg ccgccacatc
    20041 gtcgtcggac agcgggacaa gcgcgacgat gaccgagcgc gacagcagcg gcgcgacgac
    20101 tgcgaagctc gggttctcgg tcgtcgcggc gacgaggatg accgtgcggt tctcgacgcc
    20161 aggcagcagg gcgtcctgct gggccttggt gaagcggtgg atctcgtcga ggaacagcac
    20221 ggtatgccgt ccgtagaggt cgcgctctcg ggtggccgcg gccatcacct cacgcacgtc
    20281 cttcaccccg gcagtgaccg cggacaactc aacgaaccgc cgccccgacg cccccgcgac
    20341 caggtgggcc agggtcgtct tgccggtgcc cggcggcccc cagagaatcg ccgagagcgg
    20401 gccggccggc ccgctcgacc cctccacgag ccgccgtagg gggctgccca cccgcagcac
    20461 cgcctcctgg ccgcgcacct ccgcgagact gcggggtcgc atccggaccg cgaggggaag
    20521 gtccagcgcc gggtatgccg cgtccgcccc ggtcccggca ccggccccga acaggtcacc
    20581 tgacgtcaca gaattccctt caggtccgct ctcgtccgat gagaggctat cgctgtgacc
    20641 gaccggcgtt cgtctactcg gaggggacgg cacaccgcgc aggccccggg gtccgccccg
    20701 ccgcgccccg ccggtagtca tcgcacccag cggcaaccgg ggctgtcgcg ctggggagcc
    20761 tcgaccgctg accatccgtg gcgcgtggtg ctcacctggg cggtcctggc tgtcgccgcc
    20821 ttcctcgtgg cagccgccgg cgtgggtggt ccgtccctgt tcagcaagct gtccaccggg
    20881 gagcccacgg cgcccggtga agcgacgacc ggccgtgacc tgctggccgc cgccgagccc
    20941 gaccgacccg cgatcctgct caccgttgac ggcgtcgacc tggtcagccc ggccgttgcg
    21001 actgccgctg cggccgccac ggccgacatc gcggccatcg acggagtcgc cgacgtcgcc
    21061 agcccgttcg agttccccgc cgggaccgcc gacccgcgcg ccgcggcttt tgtcacagcc
    21121 gggtcgaccc cgcccaaggc cttcctcatg gtcacgcgcc tggaggccgg cctggagggc
    21181 gcggcgcagg atcgtgccga ggtcgcggtg cgggcggcat acacccggat ggggggcacg
    21241 ctcccagggg cgagcatcgc gatcggaagc ggggcggccc tcggcgacga gatcgtcggc
    21301 cagatggagg ccgatctgcg cctcggtgag ggaatcgctc tgcccctgtc cttcgcgctc
    21361 atggtcgtgg tcttcggcgg gttcgtcgcc gccgggatgc cgatcatcgg tgcggtcgtc
    21421 tccatcggcg gcgccctggc cgcgctgtac ggcttcagca ctgtcatgga cctggacgcc
    21481 gcgaccgtca acgtcgtcac cctgctcggc ctggccctgt cgatcgacta cgggctgctg
    21541 ttcgtctcgc gagcccgcga agagttgcgc gccctggccg aggggcggcc agccgcagac
    21601 ctcacccacg agcagatccg gctggcgatg gccagaacta cggcgaccgc agggcggacc
    21661 gtgctcttct ccggcgtcac cgtggcgatc tccctggccg gtctcatggt ttttcggtcg
    21721 ccgacgatcc gggccatcgg cgctgcgggg atgtcggtcg tcctcgtcgc gatgctggtc
    21781 ggggtctcga tgatgccggc cttgtgcacc ctcggagcgc ggcgcctgct ggcgcgcggg
    21841 accgagaccg cgccggacga aggggtcttc tcccgcctgg ccgcggccgt tcatcgccgc
    21901 ccctgggtgg tcatgatcgc cacgagcgcg ctcctgctgg cgatggccgt gccggccctg
    21961 tcgatgcgac tcacctcgtc gggcgtcgag ctcgtcccgg tcggctcgcc gcagcgggac
    22021 ttctacgagc ggctcgaccg cgacttccct gcgctgcggg caccgacggc gaccgtcgtc
    22081 gtcaacggca gcctccagca agcgcaggac tgggcccagg gcaccgttgc ccgactcccc
    22141 ggcgtgaccc aggcgcaggc gcgtgacctg ggcgacgggt atgccgcggt cgccgtccac
    22201 actggcgctg cgcccctcga cgaaggctca cgagccctcg tcgagacgct gcgggcgaac
    22261 cgaccggccg tccccatctg ggtgaccggt caagccgcgg ccctgctgga cctcaccgga
    22321 tcgatcgccg cgggcgcgcc gtatgccggc ctgctcgtgg tgtttgcgac cttcgtgttg
    22381 ctgttcctca tgaccgggtc ggtcgtcatc ccggtcaagg cgctggccct caacgtcgtg
    22441 tccctcggtg cctcgctggg ggcgctcacc tggatcttcc aggacgggca cctggcgtcg
    22501 gctctgggct tcacctccgt gagcgccatc gagtcgatca tcccgccgct ggtcatcgcg
    22561 ttcggcttcg ggctctcgat ggactacgag ctgttcctca tctcgcggat cgccgagctg
    22621 caccaggcgg gcgtcgagaa caaccgtgcg gtcgagctcg gcttgcagcg ctccggccgg
    22681 atcgtcacct cggcggcggc cctggtcgtc atcgtgttcg ctggcttcat cgccggcaag
    22741 atgctcatca tcaaggagat gggcgtcgcc ctcactgtcg ccgttctgct cgacgccacc
    22801 ctggtgcgca tgctgctggt cccggccacg atggagttgc tcggcgagcg gaactggtgg
    22861 gctccggcac cgttggcgcg ctggcacgcg cgacacggca tacaggaatg accgtcaggc
    22921 ggtgggcggc accggcctgg agtcgacgcc tgcctccttg cgctgctgcg gcgtgatcgg
    22981 cgccggagcg ccggtcagcg ggtcgtaacc gccaccggat ttcgggaagg cgatgacgtc
    23041 gcggatcgac tcggcgcccg agagcagcgc gcagatgcgg tcccagccga aggcgatgcc
    23101 gccgtgcggc ggggcaccga aggcgaaggc gtcgagcagg aagtggaact tctcgcgcgc
    23161 ttcctcttcc gacaggccca tgacggcgaa aacccgctcc tggatgtcgc gccggtggat
    23221 acggatcgag ccgccgccga tctcgttgcc gttgcacacc atgtcgtagg catacgcgag
    23281 cgccgagccg gggtcggagt cgaaggtgtc gatgaactcc cgcttgggcg aggtgaaggc
    23341 gtggtggacc gcagtccagg cgcccgaccc gacggcgaca tcaccggcgg cgaccgcgtc
    23401 accggccggc tcgaacaacg gcgcgtccag gacccacagg tggctccacg aaccctcggc
    23461 gatgagcccg ccgcgcttgg cgatctcgag ccggaccgcg ccgagcaggg cccgggacgc
    23521 cttggtgacg ccggccgcga agaacacgca gtcgccgggg gccgcgccga cgtgggcagc
    23581 cagaccggcc cgctcggcgt cggagaggtt cttggccacc gggccgccca gctcgccgtc
    23641 ctcctggacc aggacgtagg ccaggccgcg agcgccgcgc tgcttggcga actcctgcca
    23701 ggcgtcgagg gtcttacggg gctggctcgc gccgccgggc atgacgacag ccccgacgta
    23761 gtcggcctgg aagacgcgga acgcggtgtc ggcgaagtat gccgtgcagt cgacgagttc
    23821 ctgcccgaag cgggtgtcgg gcttgtcggt cccgaaccgg gccatcgcct cggcgtaggt
    23881 gagccgcggc agcggggttg ggatgtccac accgatgagc ttccacagcg cgacggcgat
    23941 ctcctcgcca agcgccagga tgtcgtcctg ctcgacgaag ctcatctcga tgtcgagttg
    24001 ggtgaactcc ggctgccggt cggcgcggaa gtcctcgtcc cggtagcaac gggcgatctg
    24061 gtagtagcgc tccataccgg cgaccatgag caactgcttg aacagctgcg ggctctgcgg
    24121 gagggcatac caggacccag gggccagccg ggcggggacg atgaagtcgc gcgcgccctc
    24181 gggggtcgag cgggtcatcg tcggggtctc gatctcgacg aagtcgtggc cggcgagcac
    24241 ctcgcgcgcg gcagcgttga ccttggaccg caggcggatc gccgcgccgg gcccagggcg
    24301 acgcaggtcg aggtagcggt acttcagccg ggcctcctcc cccaccgtga cgcgctcgtc
    24361 gatctggaac ggcagcggcg cggccgcgtt gagcacctcg agggtggacg cgacgacctc
    24421 gactgccccc gtgggcaggt cggcgttggc gttgccctcg gggcgcagcc gcacctcacc
    24481 ggtgatctgc acgcagtact cgttgcgcag gtcgtgtccg ccaccgtcgt cgaggacctc
    24541 gtcacgggcg accacttgga cgacgccgga cgcgtcccgc aggtcgagga acgccacgcc
    24601 gccgtgatcg cgccgcttgg ccacccagcc cgcgagggtg acggtctggc cggagtggtc
    24661 ggcacgcagg gtgccggcct catgggtgcg aagcacggta ggtgtccttc tcgggttgtt
    24721 cggatggtcg cccaggtatg ccgcccgctc gccgttgcgg ccagccgacg gaccgcgccg
    24781 gggtcggcgc ggcggtgacc ggtcaatcct aggcgggccc ggcatacccg ctcgaccgag
    24841 tttgttcgcc gagtagtcga ccgagtagtt cgcccgagta ggttcgccgg tcgggcctgg
    24901 acctgagtgt ggtttgctgt gggtggccgc agttcggtgt caccaggaga gccacatgcg
    24961 tacaggggtg cagggcttgc ggggctggcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25081 nnnnnnnnnn tagcccgtga tgagcatgac gtgtcgaggg gtgtagtcgt caccgacgta
    25141 gaacggcacc ggggtgccgt tgttcgccgc ctccgctgcg cggctcatga gcttgcggac
    25201 cagcgccggg tcctggtcgt cgtacaacac cgattcgtag gccgtgggcg agcccagggt
    25261 ggccagtgcg gctgcgcccc acggcggcgt gccgagtgct tcgggccacg tgcccagatc
    25321 gctgtcggtg gtcgtcaaca ccgaatgctg ggcggcaatg aatcgttcga ggaacgtcgc
    25381 accgttccac tgcgcctcgg tgaggtgctg gaggtaggcc gggtcccacc ctgggggccg
    25441 ttggccggac gtcagccaca gggccagcat cggatcggac cgggccagca gagcgagcag
    25501 gctcgtcgac gcacacgtgt tgccggtcgt ggggtcctgg atgacgcgat cgaggccgta
    25561 ggtcgcgcca ctggtcaggt tgtcggtgtt gagcgacatc attcgggcga tgtcctcgtc
    25621 gctcatggaa cggaccgcgt cagccatgaa gcgcagttgc tccatatcgg ctccggcggc
    25681 cagggtcgac agcagcacgg cgcgttgcgt cgatgacccg gccgcggcca acaactcgtc
    25741 gagggcggcc cgttgggccg gtgtcagggc ggccagcatg gcgcccacac gctgccactg
    25801 gtcagggttg agcaggcgat tgtcggggtc tgaggtacgc cattggtcga ccatgaccgc
    25861 gacgtccagg acggtgaagc ccggcacgcg aggggcactg gccagcaccg cccagccacg
    25921 cccgtcggag ccctcgcgct ggagggcctg caaagcggtc tccgcctgct cataggcccg
    25981 catcgcagtc cgcacacctt ctcgagcgtc ggccagggcg cggctcacct cccgggtcac
    26041 cgctaccggg tccgggatgt gcgtgtcgac accgtgcacc tcgacggtga ggtcggcaca
    26101 gcgacgaagg tgggtcacgg cgtcgtcgaa gtggtcatcg acgcggccgt gctccgtgat
    26161 cgcgtcctgc agactcgcgg cataccgtcg cagggcgcgc gccgagagag ccagcccagt
    26221 cacctcgtga ctcaggcccg aggacaacgc ggcggcagcc tgggtagcgg tcccggccgc
    26281 tactcccacg acgatcgccg gcagcccgac gcggcgccgg tccaggtcgg ctcggacctc
    26341 gtccaccgct cgcccagccg tgtccagcga cgtcgccatg ccctcgatgg tggcggggtc
    26401 gcctgggggc gggttcgaga tcgtgcgccg gaaacgctcg atgtcggcaa tgagcgacgg
    26461 tggccacacg agggcgattg ccacgtgacc cagggtccgc aggaaactcc gacactcatt
    26521 gagcaggtgg gtgacctgcc actccccgct cacgccgggt cctcgtgcgt gagggccccc
    26581 accaccatga cgaggtcggc gaagaaccgc cgaacccgat gggtgagatc cggcgactgc
    26641 ggttgtccgt cggccaaatc gaccgcgagc ggttggaggg cgtccacgag gaccgcgccg
    26701 gcccgcgggt cggtcaacag ccccggacct agtgcgagga cggcccgtag cccggccaac
    26761 tcagcagccc aggcgatctg gcgaagagcc gtcacgtcga cctgggcatc gaccaacgca
    26821 tcgcctactc gggccaggtg cagggcctca tcggtcgagg tcgtcacgcc aaccgcattc
    26881 tgtggtcggt ccgagccacc gtctcggtct gcgccctgac ggctgtggcc cagacgtcca
    26941 gggcctcacc ccgcaggtcc agttccgtgg cccaggtgtg caggagttcg cggatcatcc
    27001 ccgtcgcgtc gccttggttc acggtgatcc ccgcgagacg cctcgattgg gcctgcacgg
    27061 catcggccat ggacgccacc tggccgctcg cctcgccggc accgccctcg tcccacgtca
    27121 tcgagcccgt catcggctcc ccctgtcaca acctgttcgc gcgatcggtc tcgcacgcgt
    27181 cagccatgac cgtaaagcgt ccgcgcctcg ggcgcctgag tacgtgctac ttagactccg
    27241 ggcgactcag atcagacgcg ggctactcag actctgagct actcagactc cccgagccgc
    27301 tgctcaaccc gcctgcgggc cggtgcggtg accatcccgg tatgccgggt gctccggcca
    27361 cggggcatcg tccggtcgca gggtgctcca gccctgggcc cgggctcccc aggcggcgag
    27421 gatgcccagc agcgcgccgg ggttgtgcag ctgcccggcc agcgccgcgg catacgcatc
    27481 gtcgaggctg acccagcgga cggtgaggtc ggcctcctcg ccggtgcgca cgtgcggttc
    27541 gcccggagcg ggagcgaggt cacgggcgag gaacactcgg taggcctcgg ggaagactcc
    27601 cgccgaggcg taggcgtcga tgagcaggtc ccaccggccc gcgaccaggt cgacctcctc
    27661 gcgcagctcg cgttcggcgg cgtcgcgcgg cggctccccg gggatgtcga gcaggccagc
    27721 cggcagctcc cagaggtagg aaccgcaggc gtggcggtac tgccgaatca tgacgatgtg
    27781 gtcgaccccg tcgatctcgc gcagcgcgag gatgagcacg gcgccggggt ggtcggcgta
    27841 gtcgcgggtg acaacctcgg cgccaaggtc gacccgatcc cggcggacgt cgaagaccag
    27901 cccctgccag acgacgtcgc tcgccacgac gggcttcggc gcgatctcgt cccgcaacag
    27961 cgcggcagtg ccgatcggcg cgtcgaaggc cacctcagcc gaccgcggcg gtctgtgact
    28021 cgggtcgggt ggtgcgctcg acctcgacga ggcgttgcgc gcgctggcga tccagggccg
    28081 caccgaccaa cccggcgaag agcgggtggg cgcggttggg gcgcgagagg aactccgggt
    28141 gcgcctgggt ggcgacgaag taggggtgca cgtcggccgg caactcgacg aactcaacca
    28201 gcgacccgtc cggggacagc ccggagaaca ccaggccggc gccttcgagt tgctcccgat
    28261 aggagttgtt gacctcgtag cggtgccggt ggcgttcgct cacctcggtg gtgccgtatg
    28321 ccgtggcgac gacactcccc ggggtgaggc gggcggcata ccggcccagc ctcatcgtgc
    28381 cgccgaggtc tccggcgcct tcgacgaagg ccagttgctc agccatcgtc gcaacgacgg
    28441 ggtgggtcgt ctgcgggtcg aactcgctgg aggaggcgtc ggcgatgccc aacacggtgc
    28501 gggcgtattc gatgaccatg cactgcagcc ccaagcagat gccgagggtg gggacctgac
    28561 gctcccgtgc ccagcgcagg gcgccgagct tcccctcgat gccgcgcacg ccgaacccac
    28621 cgggcacgag gatcgcgtcg acgccgccca gggcccgctg agcgccggcc tcggtctggc
    28681 actcgtcgct ggccacccag cggatgacga ccttggcgtc gtggtggaaa ccacccgcac
    28741 gcaacgcctc ggtcaccgac aggtaggcgt cggggaggtc gatgtacttg ccgacgagcg
    28801 cgatctcgat ctggtgctcg gggtcgtgga cgcgcttgag catctcgttc cacgaagtcc
    28861 agttgacgtc gcggaagccc aggcccaggc ggcggatgac gtaggcgtcc aggccctcac
    28921 cgtgcaggac cttggggatg tcgtagatcg acggtgcatc gacggccgcg gcgaccgcgt
    28981 cctggtcgac gtcacacatg agcgagatct tgcgcttgat cggctcgggg atctcccggt
    29041 cggcccgcag gacgagcccg tcgggctgga tgccgacctg gcgcagggcc gccacggagt
    29101 gctgcgtcgg cttggtcttc agctcgccac tcggggcgag gtagggcacc agcgagacgt
    29161 gcaggaagaa gcagttgtcc cggccgatct cctggcgcac ctgacgcgcg gcctcaagga
    29221 acggcaggct ctcgatgtca ccgacggtgc cgccgatctc ggtgatgatg aggtcgggcg
    29281 cgtccttgtc gccggtcgcc aacgcccgca tccgggcctt gatctcgttg gtgatgtgcg
    29341 ggatgacctg gaccgtgtcg ccgaggtatt ccccgcgccg ctccttggcg atgacggtgc
    29401 tgtagatctg gcccgtcgtc acgttggcgg tgccctcgag gttgacgtcg aggaagcgtt
    29461 cgtagtgccc gatgtccagg tcggtctcgg ccccgtcgtc ggtgacgaag acctccccgt
    29521 gctggaacgg gttcatcgtc ccgggatcca cgttgaggta ggggtcgagt ttctgcatcg
    29581 tcacccgcag cccccggctc ctgagcagat gcccaaggct tgaggctgtc agccccttac
    29641 cgagcgagga ggctacgccc ccggtcacaa agatgtgttt cgtctgctcc gccacgggag
    29701 gccagtctat gtgatgtctg gggcgacggc ggacggcgag cgtgcgcctc ggcgcgtctt
    29761 tgctgcgccg gcccggcgag cgggcgcgac ctccgttgca gcagtatgcc gttcgcgacg
    29821 acgcgtcgtc gcctcggcca gcaggactcg ggcatgttcg agcccggcct ccgacgtgcc
    29881 gccgcccatc atgcgggcaa gctcgcgcag ccgctcgtcg ccctcgacct cgcggacagc
    29941 actggcggtc accgagccgt cgtcggactt gtggaccacc aggtgtcgat cagcgaaggc
    30001 ggcgacctgg gccaggtggg tgacgacgac gacctgggcg tgggtggcca gtgcggccag
    30061 gcgtgcgccg acatcgaggc cggccttgcc gccgacgccc gcatcgacct cgtcgaacac
    30121 gaaggtaggc acgaaggtgg gcaccagcgc cgcgggcgtg ctccccgaga cactgaccac
    30181 ctcgatggcc agcatgagcc gagacaactc gccgccgctg gctgcacggc cgaccgatcg
    30241 ggccggtgcc ccggagttgg tcgtcaactg gatctcgacg tcatcggcac cgtgcggccc
    30301 gagcggtcgc ggcgtcacgg ccacctggat gcgggccgac cccatcgcca agtgcgccaa
    30361 ctcgtcgccg acggcgttgg cgaaccgatc ggcggcggcg cgacgctgcg cgctgagccg
    30421 ctcgccggcg gccgtcacct cggcggtcaa cgccgccacc cgcgcatcga gggcggcgag
    30481 gcgttcgtcg gcagactgca gcagggccag gcgcgacgcc gcctcgccgg accaggccag
    30541 cacgtcgtcg atgctttcgc cgtagcggcg ggtgaggccg gctagatcgg ctcgccgctg
    30601 ctgcacccac gccaaccggg ccgggtcggc gtcgaggtcg gcgaggtatg ccgcgaggtc
    30661 ggccccgatg tcggccacga ggtagccggc ttcggcgagg cgctcatcga gagcggacag
    30721 tcgagggtcg tgcctggcga ccggtccgag ctgcgctcgg gccctggcga gcaggtccgt
    30781 tgcggcgcct tgctcccccg cgtcggcggc atacccgtcg tcgcccagca ggagcccgtg
    30841 ggcagcgcct gcggcatcgc gcaggccctc ggcgtgggcc agtcgggtgt cctcggctcg
    30901 cagcgcctcg tcttcccccg gttgagggtc gacacgggcg acgtgttcga gagcggcggt
    30961 gagggagtcg acctcccggg tgcggtcgcg gtctaggcca cgcagggtgt cgcgctcggt
    31021 cacggtcgcg gtgagggcgg catacccctc tcggtatgcc gcgagcgcgg cggcgaccgc
    31081 ttcgccgccg taaccgtcga ggacgtcccg gtgctcgtcg ggtctgcgca gtcgccactg
    31141 atcggcctgg ccgtggacgg cgacgagatg ctcgccgatc tcgccgagca ccccgacggg
    31201 cgccgaccgg ccgccgatgt gcgcccgcga tcggccctcg gcagtgacgg tgcgggccag
    31261 caccaactcg tcttcgtcga gctcaccgcc ggcgtcctgc gcccgctccc gggccgggtg
    31321 tccggtgggc agtcgcacga tgccctcgac cgacaggctc ggccgaccgg agcgcacgag
    31381 ggcaggatcg gcgcgggagc ccagcagaag cccgagcccc tggacgacca tcgtcttgcc
    31441 ggcgcccgtc tctccggtga ggaccgtcag gccggagtgc agcctgaggg tggcctcggc
    31501 gatgacgcca agcccggcga tgcggatctc caggagcacg ggcccaagac taagggcccg
    31561 cgccgtcagt cggtgggtgg ctgtggacgg cccgcgccgt tcccgcgcca gccagccacc
    31621 gacaggtcga acttctcgac gagccggtcg gtgaacggcg cgtgcgacag ccgggccaga
    31681 cgcatcggca cggccgagcg gcagacgtcg atccgcgcgc ccggtgcgag gtccacgtgg
    31741 cgtcgcccgt cgcaccagag gacaccctgg ccctcggcgt cgcggatgac ctcgacggcc
    31801 agccgggatc gaggcccgac gacgaccggt cgagcgaaca gcgcgtgggc cgagttgggc
    31861 acgagcagga tcgcttcgac gtccggccag acgaccggtc ccccggccga gaaggcgtat
    31921 gccgtcgagc ccgtcggcgt cgcgatgatc accccgtcgc atccccaggt ggacaacggt
    31981 cgaccgtcga tctcgacggt gacctcgagc atgcgctccc gggcggcctt ctcgatgctc
    32041 acctcgttga gcgcccaggt gcgggagtcg acgcctgcgc catggctcac ggtgacatcg
    32101 agggtcatcc ggtcttccac ggtgtagtcg cgagccacga tccgatcgac cgtcgtcgcc
    32161 aggtcggcca tctcgacctc ggcaaggaaa cccaccttgc ccaggttgat ccccagcaga
    32221 ggcgtgtcgg tgccccggga gagttcggcg ccccgaagga tcgtcccgtc accgcccagg
    32281 acgcacacga gctcacagtt gttggcggcc ccggcgtcgg gcaccaccgt ggtcggtccg
    32341 atgtccgtgg cgtgcagcgc tgccgcatcg gtggcgatca tcgtcgtggc caccccttgg
    32401 gcgcccagtc gggtcgcgac gtcacgggcg atgtcgccga tgtcggggcg tcggggatga
    32461 gggacgagca gcacgcgtcg ggtcacgagg gtgactccat ggcggcgacc agcctttcca
    32521 ggtccgcggc ctcggcgcgg tcgtgcgcgc gagatgtgaa ccagcctaga tactcccggt
    32581 tgcccgtggt gccccggatc gggctcgcga ccagcccacg cacaccgagc cccacgtctg
    32641 tggcggcgtc gagcactccg cgaagggcgg ccagccggtc agcgtcgacg cgaaccacgc
    32701 cacccttgcc gagccgctct cgaccgactt cgaactgcgg cttgatcagc aacaccagat
    32761 cgccgctcgg ctcgatgagt gtggccagga ctggcaggac gaggcgcaag gagatgaacg
    32821 agaggtcggc gacgacgagg tcgaaggggc cacccaagtc ctcggcggtg cagtgccgga
    32881 tgttggtgcc ggagcgctcg atgacgcgcg ggtcgccggc cacctgttcg actagttgcc
    32941 cgtgaccgac gtccagggcg gtcacctggg ccgcgccctg actgagcagg acctgggtga
    33001 agccccccgt gctggcgccg acgtccaagg cgcgccgacc cgccaccgtg aggccctgcg
    33061 gcccgaaggt ctccagggcg ccg
//
LOCUS       DAOMZY010000005        32257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_1050_length_32257_cov_5.719614, whole genome shotgun sequence.
ACCESSION   DAOMZY010000005 DAOMZY010000000
VERSION     DAOMZY010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 32257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32257
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1050_length_32257_cov_5.719614"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            291..1265
                     /locus_tag="PLK64_00630"
     CDS             291..1265
                     /locus_tag="PLK64_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconolaconase"
                     /protein_id="HPV78274.1"
                     /translation="MPRHTVYARTLLTAALCASLGAALTAAPAHAEGRPTAYQLSGDA
                     ASPVGSKFEGIGADERRGLFYVSEVTGGEIHRGSALTADTQEWLAGDGTDGRFTARGI
                     TVDDAGRVYIAGGPNGIGTGRPDLWVYAPDGTLLAALRAPGAGVFLNDVAIGPDGAAY
                     FTNSNDAQVFRVANDGAGWQSTLWADATDVITRETGFNLGGIVLTADRSAFVVAQGNV
                     GKLWRFDLASSEATLVATGDANLINADGLVREGNTLTVVRNFSRMIATLQLAADGTGA
                     RLVDQQATASDRVLTTAKTLHGRILYVDSKFDEAIASGPYEIITDPTR"
     gene            1262..2521
                     /locus_tag="PLK64_00635"
     CDS             1262..2521
                     /locus_tag="PLK64_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPV78275.1"
                     /translation="MSHSPHAVLATLLVALALTACGGGATATVPVQPPTSLAQTPTTP
                     VPTPTTRSAELTTLNEGLRAAAWANDVTEARRLITLGADVNAKDETQQSAYLIATSEG
                     YLDLLRLTLAHGAQVDDKDSWNGTGLIRAAERGHALVVGELLRAGINRDHINRIGYQA
                     IHEAVWLGDDTPEYATTVRVLAAGGVELDRRSPSAELTPLEMARQIGHTGAAGILETI
                     TSAARPADPDAALLQAAEVGDANAVAIALRAGANIEARDAHDRTALLLAATYDQIPVA
                     NVLVAMGASPNALDDRHDTPWLVTGVTGSVAMLEALLPANPDLKIRNRFGGLSPIPAS
                     ERGHIDYVRRVVLTGVDLNHVNDLGWTALLEAVILGDGGPNHQEVVQILLAAGADRDI
                     ADRAGVSALQHAEQRGQNEVARLLRQR"
     gene            complement(2614..2913)
                     /locus_tag="PLK64_00640"
     CDS             complement(2614..2913)
                     /locus_tag="PLK64_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011775781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV78276.1"
                     /translation="MSSLAAALEVVGARWALLIVDRLLDGPQRYGDLQRDLGVPTNIL
                     ATRLRELEAAGVLSRLPLRHNTRAYALTDRGLALREAIVALGNWGAEDPTGGSAE"
     gene            complement(2910..3314)
                     /locus_tag="PLK64_00645"
     CDS             complement(2910..3314)
                     /locus_tag="PLK64_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015623914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPV78277.1"
                     /translation="MSLFITCPVESVERATAFYTALGWTLNAEMSDHNVSCFAIAPEQ
                     YVMLGSREMYASVGGVEELIGGPDTPSKVTVSFDLASREAVDEIVDRAAAAGGRVGDT
                     DDYPFMYQRQFDDPDGYHYSPFWMKPDADPTA"
     gene            complement(3401..4372)
                     /locus_tag="PLK64_00650"
     CDS             complement(3401..4372)
                     /locus_tag="PLK64_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPV78278.1"
                     /translation="MLAVYAASQDRANPLSGLVVGDRPAPEARPGWSVVTLRTAGLNH
                     HDVWSLMGVGLSADKLPMILGCDGAGVDESGREVIVHSVVASDGWSGDETLDPGRTLL
                     SELHPGTLAEQVAVPTRNLVAKPEGMPWETAACLSTAWLTAYRMLFVNGGLTPGATVL
                     VQGAGGGVATALIQLGSAAGLRMWVTSRDEAKGARALEIGADRAFGSGERLPDRVDAV
                     METVGAATWSHSVNSLKPGGAIVISGATSGDAPAKAELTKIFFKQLRVIGSTMGTKAE
                     LERLAAFVQGKGITPVIDSVRPLAEAREGFEHMANGDVFGKVIFTVS"
     gene            complement(4434..6074)
                     /locus_tag="PLK64_00655"
     CDS             complement(4434..6074)
                     /locus_tag="PLK64_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HPV78279.1"
                     /translation="MFEGLPWEVGQDQLEVLAGRSLRDVSPLLAADALLGVDPLALLD
                     LDQSVAEEVVAQLQVALNQVTALQAIVIETVARRVEHDVELAKDGDRLAGHFPAGWGT
                     GDDYTPSVLAPLLHLPPRTMAGRLGEARVLVNELPRTLRAALDGELEPWRVGAVVAGS
                     FGVASERMPDFEVALFGEGGLGDATAGVLRKRAERVAAKVDAGVLEQRVAEGKRSRCV
                     RVAPGVLPGTTEWTAIAAADRSQLAWSAVCDLADEYLRGKPGLTPGQARADAFLDLLL
                     RNVDVQTIVTIGVTDATIVALLEANEQVQGSGQRGSGQQGSGQQGQAFRQPQFVGVGA
                     ASIPSPALPYLPPDVHPPFTRTALTTRLFSEPADQAAGTRVDDSLAGSAQAGLAAPAT
                     VVSGGAQPAAGSGVVDAGACGWLLGDQVAAILRHPDTLVRLAKLDPRTGATAYLSSES
                     YRPGAALARLVRERDGTCRFPGCRVAAARCDLDHAVPFPQGPTDARNLHALCRRHHGF
                     KHHAGWSVDLAWDGTLTWTSPTGRQYVTLPHDRRESAA"
     gene            6271..7983
                     /locus_tag="PLK64_00660"
     CDS             6271..7983
                     /locus_tag="PLK64_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HPV78280.1"
                     /translation="MTSPSSATTPAPTRTPTAVDAIAENYLARYAALSPIEATYLGLP
                     GHDEDLDDLSPAGYAAMSALRRETLASLADAVAVDDIDLVTIASMRDRLGVAEEKYAA
                     GMDEMSLNVIASPLQNVRDAFDLMPQDTDEHWRTVAIRMAKVPTAMAQYAESLTLARS
                     RGDVAPRRQVDACIVQARDLTADDGYFVTLVAEAKAGDTALEPEVASDLAAAAEAARA
                     AYVQIADFLETELAPVAPAADACGRERYALHSRDFVGAVVDLEETYRWGQEELARITA
                     QMQEVAEQIKPGATIEEAIEVLSADPAYLLEGTDALRTWMQGKADEVITNLADVHFDI
                     PGPVRTIECRIAPTQTGGIYYTGPSDDFTRPGRMWWSVPKGETTFYTWSELSTVYHEG
                     VPGHHLQVAQTVFRRELLNTWRRLASWTSGHGEGWALYAERLMSELGYLDDPGNRMGW
                     LDAQSLRAARVVIDIGVHCGFEAPAEVGGGEWTYDKAWAFLTAHAHESEGKLRFELDR
                     YLGWPGQAPSYKIGERLWLSLREQVRERDGADFDVKAFHRKALDLGGVSLDTLRDAVL
                     GNLG"
     gene            8034..9932
                     /locus_tag="PLK64_00665"
     CDS             8034..9932
                     /locus_tag="PLK64_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015938473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HPV78281.1"
                     /translation="MTLAIAVVALVATVLTVTELARRLDVSAPLLLTIVGVGASFLPF
                     VPPLVVSPELVLVGLLPPLLYAASVKTSLVDLGANRRNIGMLSVGLVAFTAIGVGLAL
                     WWLFDVPLPLGIAVGGVVAPPDAVAATAVGRRIGLPRRIVTVLEGESLFNDATALVLV
                     RLGILALGSALSVGAVVGQVAWTSIGGVGIGLVVAQVLTWVRRRVTDPISGTAISFLA
                     PWLAYLPAEELHASGVLAVVVAGVLLGHRAPVIQSATSRVADRINWATAQFILENGVF
                     LLIGLQAKGIIERITATPAGVSRALFMSVVVLAAVVVLRPLWVIPLRFLMGDPQDRRD
                     QLRSSVIVSWAGMRGVVTLAAAFTLPVGVPDRDAFVLAALVVVAGTLLVQGSSLPWVA
                     RRLDVHGPDPREDVLQTATVMRSAVTAGLRELDRLAAPTEPPLPDGLVDQLRTMAERR
                     VHAVWEQLGAPSGTERPDGSGGAMVETPSESYRRARTAMLQAERTEVLRLRDLGTIDH
                     EVLAAVLASLDVEESVVTSVSERQGALRERLVLTPESSKGDCLHLRDAPVGIEPVTPD
                     GCADCLRDGTTWVHLRLCLTCGNVGCCDSSTGRHATAHHRQTAHPVMRSIEPGEGWRW
                     CYVDELLG"
     gene            10089..10745
                     /locus_tag="PLK64_00670"
     CDS             10089..10745
                     /locus_tag="PLK64_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HPV78282.1"
                     /translation="MTTARFRLVLASASPARLNTLQHAGVEPTVVVSDVDEDAAVAEA
                     LERYGPLEPAQVALVLARAKAEAVAFGERVGDLVLGCDSVLEFDGQMHGKPADADEAR
                     ARWRRMRGRHGVLHTGHWLVDQRPVERGGSQATLGATASTKVYFAKLTDEEIDAYVAT
                     GEPLRVAGAFTIDGLGGPFVTGLEGDHHNVVGISLPLLRELLIDIGVNWFDVVTVHQR
                     "
     gene            complement(10779..11303)
                     /locus_tag="PLK64_00675"
     CDS             complement(10779..11303)
                     /locus_tag="PLK64_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78283.1"
                     /translation="MVDTSLAHSADGILRSIEFAAEPVRVNRAGKPRPPRAVIWALSL
                     IGAAAVAGASATIYWSGAGLPIANASLSMVVSVADERAGGVVQKAAFVAAAVGVASYL
                     LVILGLSRRSTWARRVATLFAVIGGLGVFTVSYAAASALGLVGVALLWLPASSAWFRA
                     QPIPVVPAPQDQAP"
     gene            complement(11728..13014)
                     /locus_tag="PLK64_00680"
     CDS             complement(11728..13014)
                     /locus_tag="PLK64_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78284.1"
                     /translation="MAGAFTAPIPPEEAVPRTADAPGTPWWRRFPLWFPLAVYAVTRL
                     IDAVYFAIMQRSQIPMVPGNGAARVIFPTPAAPGYWPLMANWDGQWYHEIAELGYPDP
                     IPTDPVTGKVVQNPWAFYPMFPMLSRLVMRATGANFYVAGALLSTVVGAIAVVLMFRL
                     VDRAAGRWSAIVATVATSTWVAAPVLQTDYSEACALLVIVLLLTALRGRRYWWVLVLL
                     VALALTRNLVLISAPPLIAHTAVRWQRRDADPFPVRQRVGLVVLTLLSGALTFLWPTI
                     VRLVTGDKDGYAKTMGAWGVDAFEIKLHTWVDYIYYDYGYLGWAACLAGIGWFAWKML
                     SVDTARWGPELWGWAGAYPAYMVLVTGIGPSRIRYALLAWPVFLLLAWILNRPRLRPR
                     RAWWLTAYALALVALQGWWVWNYWVILGLYPDVQFP"
     gene            complement(13094..14695)
                     /locus_tag="PLK64_00685"
     CDS             complement(13094..14695)
                     /locus_tag="PLK64_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPV78285.1"
                     /translation="MPPSTDPTEAPFAGPSASEGGDPRVDSVRERFAAAYLATSAPLT
                     ESLGAKLVQQNKLYVRDRIALLFDDGSFVEDGRYANALAGDLPADGVVTGRGTVDGRP
                     AIVIANDPTVKAGSWGARTVEKIVRATEMAMREELPVFWFVDSAGARITDQVEMFPGR
                     RGAGRIFHNQVALSGKVPQICCLFGPSAAGGAYIPSFCDLIIMVEGNASMYLGSPRMA
                     EMVVGEKVTLEEMGGARMHCTVSGVGDLLAHDDQEAIELAKLYFSYVPGCWRERPPTY
                     TPEEPSEPLTRHTVPERESQPFDIHTVIDGLVDADSFYPIKPLFAAELVVGFGRMNGE
                     TVGIVANNSAVKGGVLFTDSADKATRFIWLCDAFNVPLLYLADVPGFMIGSEVERGGI
                     IRHGAKMVSAVSEATVPQFCIVVRKAYGAGLYAMGGPGFAPDATLALPTAKIAVMGPE
                     AAVNAVYANKIASIADPDERAAYIAERRAEYEEDVDLERLAADLVVDGVIEADALREE
                     LLHRLRYASRRDRHFSERRRSVPPV"
     gene            complement(14748..15953)
                     /locus_tag="PLK64_00690"
     CDS             complement(14748..15953)
                     /locus_tag="PLK64_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPV78286.1"
                     /translation="MFELSADHEEFRALVREFAEAEIEPRVAQWDKDSHFPAELIPKL
                     GELGLFGLVVPEEFGGTRRPEGAGPEVSGGDFTMLCVAIEELGRVDQAIGITLEAGVG
                     LGINPILNYGTPEQKAHWLPDLAAGRALAGFGLTEPDAGSDAGGTKTKATLADGIWTV
                     NGAKSFITNSGSPITSVVSVTARTGTRNDGTPEISAIMIPSGTKGFDIAPPYHKLGWH
                     ISDTHGLSFQDVQVPEANLLGQRGKGFAQFLATLDDGRIAIAALATGCTRRMLEEATA
                     YSKTRLAFGKPIGTYQGVAFQVSDLAVLAETSRLLTYKAAWLKDEMDAGRRTMQEVKQ
                     AASIAKLYTSEAAVTATRIATQVFGGNGFMEEYPVARFYRDAKILEIGEGTSEVQRML
                     IARGLGLPA"
     gene            16073..16468
                     /locus_tag="PLK64_00695"
     CDS             16073..16468
                     /locus_tag="PLK64_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPV78287.1"
                     /translation="MASGRDGERTWSIAQVAAEFGVTHRTVRHYEDIGLIAPERHGTR
                     RVFRRRDRTRLALILRGKRLGFPLEEIRTIIDLYDAPRGKTSQLEYAITQARKRRVDL
                     EERRRDIDESLAELGAFEASCQADLDRLS"
     gene            16592..17068
                     /locus_tag="PLK64_00700"
     CDS             16592..17068
                     /locus_tag="PLK64_00700"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HPV78288.1"
                     /translation="MGFFNNAKLEPTPELAPLTKERIKATLEASSWSYHVDSDGDIGS
                     GWDHGVIWFFTYGNQAEILQVRGIWDGKLSPDDLSQALDVVNEWNCDRLFPKVYARIN
                     DEGQLRVISEHTLDYEHGLTDEQLNLHVNTGIATSCQLWERLNEAFPEAAAAAAAE"
     gene            17112..18932
                     /locus_tag="PLK64_00705"
     CDS             17112..18932
                     /locus_tag="PLK64_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HSP90 family protein"
                     /protein_id="HPV78289.1"
                     /translation="MSQRFRVDLGGMVDLLSHHLYSGPHVYLRELLQNAVDAVTARLA
                     EDPAAPATIRLTSDTEADGATVLVVTDSGVGLTSDEATELLATIGRSSKRDPHGLGVA
                     HYLGQFGIGMLTAFMVADRIEVTSRSARPGTVPTVWVGRSDGTFEVSDLPPGPSADAT
                     AVGTTVRLWARRGSEHWLETSTVVALATDYGSLLPFDVAVRVPVEGSDPTWRRITDPV
                     LPWQARHPSAEARSRALTAYCERVLGFTPLGHIDLSVPLAGVSGVAFILPQAVTPGSG
                     RHRVYSKRMLVGARVDGVLPDWAFFTRAVLDSDALSPTASREQIHDDEILLATRDALG
                     AQLKEWATATLTTPSTLSRRFVETHHLALRSLALTDDEMLDLAARVLPFETTDGARTL
                     RDVAEDGEIVFTSTTEAFRRVAAVARAQGLVVVNAGYVYDSDLLTRLGRRPGWAVREL
                     SSEDVTQVLTPVATDRAGQLAQALSMAEKVLEDEDCHVLVRTFDPDSLPAVLLRDPDG
                     EHRRALAREREATPGLWGGLLDSFETDTSTRTRSLVLNDSAPVVRQLLAAPETIVFAA
                     GLRSLYLSAVMLAGEGLRSSEVAGLHDALSVLLARSLDHD"
     gene            19006..20970
                     /locus_tag="PLK64_00710"
     CDS             19006..20970
                     /locus_tag="PLK64_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78290.1"
                     /translation="MAPSDAEVRRELREIRAMPYGSARTAAAEVIVRRIDAEGPQERL
                     AEALLDLVEAYSFTGQGHRSFVAFARLLRLWDSQPEVFDESDEHNLFWEFKWVAGDLP
                     DFPEITREQADAFLADMRRRFDLAGKGSGSVLGSRFRWAWHSGDPSMEAARLAWLALP
                     KDDMDSCAACRIGHQVDYLTETGRFEEAIELGLTQHSSCNLEPTRTLHALALAHLNVG
                     ADGPALAAYRRALATLNTSDSDYAPARGQGFEMVARGGHVERALRFLREDHPELLEGA
                     STPLFRLRFLLGVLAGLSANLDHSDLPTGLRSPDAPTLGQLHAWVHEQTAELAGRFDA
                     RGGSDYYARLLDRSLAATRTPVPLDFDISAMASLGPQPGAGDPTHQPDADPAPAALGA
                     DGVAAALTIAERHARARSYPEAAAAYLRAAEVATAAGLLEHAGMAWAESAQCAAQVGD
                     EVTAHARYAAALPLLRAADGDPGAMAQILAAWAPVAAGLGDTDALMTHLDTLLSSDGS
                     HEALLDSDLEERQRRQSENLQASLLETWARVVASRSPGVDGQIDGQVAGQSARDAADA
                     ATRAGELFAQVGRIGSAAQAFWLAGQILREDGDLDGAVLALESAYEGLTIARDAANRA
                     RAASDLIDVLRATGQHDRADQVIATLTSGS"
     gene            21091..22884
                     /locus_tag="PLK64_00715"
     CDS             21091..22884
                     /locus_tag="PLK64_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HPV78291.1"
                     /translation="MAKISKVLIANRGEIAVRIARACKDAGIGSVAVYADPDRDALHV
                     KVADEAYALNGSTPGDSYLVQEKLLAIAKEAGADAVHPGYGFLAENAGFAQAVIDAGL
                     IWIGPGPAAIDSLGDKVKARHIALKANAPLVPGTSDPVTGPDEVVEFAKAHGLPVAIK
                     AAYGGGGRGLKVARELEEIPHLYESAVREAVTAFGRGECFVERFLDHPRHVETQCLAD
                     QHGNVVVVSTRDCSLQRRNQKLVEEAPAPFLTEAQHDELVRASKAILTEAGYVGAGTC
                     EFLVGPAGDISFLEVNTRLQVEHPVTEEVTGIDLVREQFRIAEGQSLDYPDPVPHAHS
                     IEFRINGEDAGRGFLPAPGTVTVYKTPSGPGVRVDSGLVEGDTIGGAFDSMLAKLIVT
                     GRDRQQALERSRRALAEFEVEGMPTVIPFHRAVVADPAFAPADPDEAFSIHTRWIETE
                     FNNTIPAYAGGPASLPEAEERQKLVVEVGGKRIEVSLPGGLSLGGGTGGSGSGATRKA
                     APKRSGAAKSGAAASGDSVTAPMQGTIVKVAVEEGQTVESGAIVVVLEAMKMEQPITA
                     HKAGVITSLKATVGETVASGTVLAEIKDAEG"
     gene            22884..23078
                     /locus_tag="PLK64_00720"
     CDS             22884..23078
                     /locus_tag="PLK64_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78292.1"
                     /translation="MPTEGFSFVNLAILAVVALIVVPGYRHLRGTVSARRRERWAQEE
                     ADYRAAVENEDPGQDHPAGA"
     gene            complement(23094..25088)
                     /locus_tag="PLK64_00725"
     CDS             complement(23094..25088)
                     /locus_tag="PLK64_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78293.1"
                     /translation="MSWPQLLPAVLVAVAVFFVPGAVLLRSVGIGWLTAVLAAPAVSV
                     AVIIAAGLGVRAVGVGWSAVTGLIAVVLVVALGLGATRAIRSRWPAARASAAEAAPLR
                     AVLAVGAVSLAAAYLVLAGGAPSPMAIPQMPDVIFHLGAVEWMVRHGSASALDTYQYA
                     QLDSPLTYPGAFHAVTAATVAWTGLPVTATWHALLIVVVGLVWPFGVMLLARVVLGRG
                     TGILVAAGLLTLVFTSFPTRFLAWGPLWSNLLANALLPAVLAGALCAVAPSAMLAERA
                     FASGRVRAVGYAALGLLVLLGTQPNAVASGVVIGLTVVAAALPQWRHALGGRWGARFV
                     RLPWFLLMVAAAVMASTRFIPTKMYVINSHVRATWALAGSQAATLFDGSWLESGSVVV
                     LVLVGAVWLARDRNRRWLVVSLALFIAIFLLLYAVNGPVVRQLTWLWWNDHYRVRAAL
                     VLPAVLCATAGAVVIGGWVARRRPDPPRVRQGLIALAAVAVVAALGCGIPVHREVVEA
                     TYGEDSADYSWVLPREYAALSQLAAFVPSGSVVAANPYRGGMFFYVAGGTEVLYPTEN
                     SLQLPDRRLLGTSLQAVTSMPDVCAASRRQRVDYVLTGGDMHIWGVLDHTPEYSGVDS
                     AAYDPDFKVVAEAGPYALRRVPDCSTAAPGSGFIGPVTIP"
     gene            complement(25106..26503)
                     /locus_tag="PLK64_00730"
     CDS             complement(25106..26503)
                     /locus_tag="PLK64_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone dehydrogenase"
                     /protein_id="HPV78294.1"
                     /translation="MKSRTWSVVIVGGGPGGYEAALVAARLGASVTVVDRDGVGGSCV
                     LTDCVPSKSLISTAHVLRRMGAAREHGIDLPQRPRATDLRPVNERILDLAAAQSADIH
                     ARLEADGVRVCRGTGRLLAADRVLVSLVDGGTEELVADVVLIATGATPRVMPTALPDG
                     DRILTWAQIWGLTEIPERLIVVGSGVTGAELAQAYLGLGSAVTLVSSRHQVLPGEDSD
                     AAAVIEEVFRREGMEVLGRSRMSAVERTADGVIVRLEDGRTVEGSHALLAVGSVPQTA
                     GLGLEDVGVEVGPGGHVLVDGVSRTSVRGVYAAGDCTGVLPLASVAAMQGRIAMWHAL
                     GDAVAPLRLDVVSANVFTDPEIATVGVSQQDVDAGTVDAIVITLPLTTNARAKMDGFH
                     DGFVKIFARSGHGTVLGGVVVAPRASELIYPLTIAVENGLTADQLSNAFTVYPSMTGS
                     IGEAARRLHVAARMS"
     gene            26694..27833
                     /locus_tag="PLK64_00735"
     CDS             26694..27833
                     /locus_tag="PLK64_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPV78295.1"
                     /translation="MRSTSIRYAALSVAALAAVGIAACSPPPSSTSTSTTAAGGGVNA
                     AKATSLADFGGMDGLVAAAKKEGALNVIALPPDWANYGEAIKGFTAKYGIPITSDQPD
                     GSSADEISTAQRLKGQSKAPDVFDLGSAVAMANTGMFAPYQVATWKDIPDNAKDPKGV
                     WVYDYAGYMSVGYDSAKVPAPTALADLLKAEYKGKVALNGDPTKAGAAFAGVQMVSIG
                     QGGTAADIAKGVDFFKSLKDAGNFLPLDPNEASVLSGQTPVVIDWAYNNAPLTKKLPT
                     WKTWIPDNAVLAGYYIQAINADAPHPAAARLWQEYLYSDEGQNIWLKGAAFPVRYDAM
                     KAAGKIDAAAQAALPAVNGKVVIPTTADNDAAKAYLAANWTKAVG"
     gene            27889..28716
                     /locus_tag="PLK64_00740"
     CDS             27889..28716
                     /locus_tag="PLK64_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPV78296.1"
                     /translation="MAPFFVYTTVFLLIPTIVVVVGAFADGSGRPTLDNVAALGGANS
                     SFLPALQHSVVLSLVTALAGAVIGAVLAYAVSTARPDGLLKRFITSLCGVLAQFGGVA
                     LAFAFMATFSIQGFLTVWLNSLGLDTGSSIWLYQWDKGLMLVYLYFQIPLMLIVFLPA
                     VEGIKPQWREATETLGGTTWTYWTKVAGPILLPSFIGATLLLFTNAFSAYATAAALIS
                     QASPIVPLQIAGTFTSEVILGQENVGKAMALAMVVVVALVMWVYAALDKRASRWLVR"
     gene            28730..29533
                     /locus_tag="PLK64_00745"
     CDS             28730..29533
                     /locus_tag="PLK64_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPV78297.1"
                     /translation="MSTRHTERRKRAALRWTVIGLTLVFFLIPLYGMLEFTTRDLING
                     GRTGKFWTTLVDVPQVTERYPALAEGFASSMALALITVVVMLLLLVPTMTWVRLRLPG
                     LTRTVEFICLLPLTVPAIVLVVGLTPVYAWVTYLLGEGTVWLGLAYVILVLPYAYRSL
                     DAGLRAIDVKTLSEAARSLGASWLRVMWQIVLPNLRTAVLSASFLSVALVLGEFTIAN
                     LFSRKNLQVAMFELGKSDGQLSVAVGLASLVFAFVVLFAMSFVGRKEKS"
     gene            29530..30636
                     /locus_tag="PLK64_00750"
     CDS             29530..30636
                     /locus_tag="PLK64_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV78298.1"
                     /translation="MTATPSPAASRGTEVRLTDLTRVYPGNVRALDGFTLHIAPGEFV
                     ALLGPSGCGKTTALRLLAGLEDADGGTIEVGGRDVTNVPTSKRDMGMVFQAYSLFPHM
                     TARQNVEFGLQLRKQDKATRHGRAADMLDLVGLSKQADRYAHQMSGGQQQRVALARAL
                     AIEPQVLLLDEPLSALDAKVRSQLRDEIRRIQLEVGTTTLFVTHDQEEALAVADRVGV
                     MNAGRLEQLASPAQLYKNPATPFVADFVGLTNRLHCRVAGGVAQVLGGAVPLLAGSAG
                     DGLAIVLVRPEGVTIAPVGSGGSGGSGGAAATVVSASFLGASGKIVARLESSEGVGDL
                     VSVQVDNTDVTAYAPGQRVLLAPRGDAALAVSAT"
     gene            30713..31555
                     /locus_tag="PLK64_00755"
     CDS             30713..31555
                     /locus_tag="PLK64_00755"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPV78299.1"
                     /translation="MTASLDLADPNTDPFEVAAAAAAVIAERTGAERHDVALVLGSGW
                     GRTGDLIGQTLVTIDNADVPGFRKAAVEGHSGSMRSVAIGDTGKRALVFGTRTHFYEG
                     HGVRAVVHAVRTARAAGCTSIVLTNGCGGLNPEWGPGTPVLIRDHLNLTAASPIEGAN
                     FVDLTDLYSERLRELAKRVDPGLDEGVYVQFRGPHYETPAEVQMARILGGDLVGMSTT
                     LEAIAARQAGLEVLGISLVTNLAAGISESPLSHEEVLEAGRAAASRCGALLAKVVTKI
                     AQGS"
     gene            31561..>32257
                     /locus_tag="PLK64_00760"
     CDS             31561..>32257
                     /locus_tag="PLK64_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HPV78300.1"
                     /translation="MTADSTVPEADPTLFAAAEAWLADDPDASTRAELAAVLEAARSG
                     DGGAGDEAAAAAVADLADRFAGMLQFGTAGLRGRLGAGPNRMNRAVVIRAAAGLTAYL
                     KHDADHPQTEPFVVVGYDARHKSDEFARDTAAVVTAAGGRAVVLPHVLPTPVLAFAIR
                     YLGADAGVMVTASHNPREDNGYKVYLGDGSQIVPPADALIAARIAAVGSVADVPLATD
                     GWETLGPDVLDAYL"
BASE COUNT         5051 a        11507 c        10990 g         4709 t
ORIGIN      
        1 cgtcgaagcc gtgccggtcg acgtaccgca tcgccgacgt tgccagcagg accacgagga
       61 ccgcgagcag ccaccggtca agccgcgctg ccggggcgag tatgccgtgc acgccagcag
      121 cgggtgctgg ctgcggcggt gtcgtgctca tggttgaccc actctaggga gcacggagga
      181 gcccgacatc aaccaactgg ctatcgaacg gtagccagct ccccgacgtc aggcggggcg
      241 cccagcccgc acagtggtgc catcacccgg atccacaccc aggagagctc atgcctcgcc
      301 acaccgtcta cgcccggacc ctcctcaccg ccgccttgtg tgccagcctc ggtgccgcgc
      361 tcacggcggc tccggcgcac gccgaggggc ggcccacggc ataccaactg agcggggacg
      421 cggccagccc cgtcgggtcg aagttcgagg gcatcggcgc cgacgagcgc cgcggcctct
      481 tctatgtgag cgaggtgacc ggcggcgaga tccaccgcgg atcggcgctc accgcggaca
      541 cgcaggagtg gctggccggc gacggcaccg acggtcgctt caccgcgcgc ggcatcaccg
      601 tcgacgacgc cggccgggtc tacatcgccg gcggcccgaa cggcatcggc accggtcgcc
      661 ccgatctgtg ggtctacgcg cctgacggca cgctgctggc cgcccttcgt gcgcccggtg
      721 ccggggtttt cctcaacgac gtcgcgatcg gcccggacgg cgcggcctac ttcaccaact
      781 ccaacgacgc ccaggtcttc cgggtcgcga acgacggtgc cggctggcag agcaccctgt
      841 gggccgacgc gaccgacgtc atcacccgtg agaccgggtt caacctcggc gggatcgtgc
      901 tgaccgccga ccgcagcgcg ttcgtcgtcg cccagggcaa cgtcgggaaa ctgtggcgct
      961 tcgacctcgc ctccagcgag gccaccctgg tcgccaccgg cgatgccaac ctgatcaacg
     1021 ccgacggttt ggtccgtgag ggcaacacac tgaccgtcgt gcgcaacttc agccggatga
     1081 tcgcgaccct ccagttggca gccgacggca cgggagcccg acttgtggac cagcaggcca
     1141 ccgcctccga ccgggtcctc accactgcga agaccctcca cgggcggatc ctctacgtcg
     1201 acagcaagtt cgacgaggcg atcgccagcg gcccctacga gatcatcacc gacccgacgc
     1261 gatgagccac tctccgcacg ccgtcctggc gaccctgctc gtcgccctcg ccctgacggc
     1321 gtgcgggggt ggggcgaccg ccactgttcc cgtgcagccc cccacgagcc tcgcgcaaac
     1381 ccccacgact cccgtgccaa cccccacgac ccgttccgcc gagttgacca ccttgaacga
     1441 gggcctgcgg gcggccgcct gggctaacga cgtgacggag gctcggcggc tcatcacgct
     1501 gggcgccgac gtcaacgcca aggacgagac ccaacagtcg gcctacctca tcgccaccag
     1561 cgagggctat ctcgacctgc tcaggctcac gctggcccac ggggctcagg tcgacgacaa
     1621 ggacagctgg aacggcaccg gcctgatccg ggcggccgag cgcggccacg ccctcgtcgt
     1681 cggcgagctg ttgcgcgccg gcatcaaccg cgaccacatc aaccggatcg gctatcaagc
     1741 gatccacgag gcggtctggc tgggcgacga cacaccggaa tacgccacga ccgtgcgggt
     1801 tctggccgcc ggtggggtcg agctcgatcg gcgctcacct tcggcggagc tgaccccgct
     1861 ggagatggcc cgccagatcg ggcacaccgg tgcggcagga atcctcgaga ccatcacctc
     1921 cgcggcgcga ccggccgatc ccgacgcggc actcctgcag gccgcggagg tcggtgacgc
     1981 caacgccgtc gccatcgcct tgcgcgctgg cgcgaacatc gaggctcggg acgcgcacga
     2041 ccgcaccgcg ctcctcctgg ccgccaccta cgaccagatc cccgtcgcga acgtcctggt
     2101 cgcgatgggc gccagcccca atgcgctcga cgaccgccac gacaccccct ggctggtcac
     2161 cggcgtgacc ggaagcgtcg caatgctcga agctctgctg ccagccaacc ccgacctgaa
     2221 gatccgcaac cgcttcggcg gcctgtcacc gatccccgcc agcgagcgag gccacatcga
     2281 ctacgtgcgg cgggtggtgc tgaccggcgt ggacctcaac catgtcaacg acctcggctg
     2341 gaccgcgctg ctcgaagcag tcatcctcgg cgacggcggc ccgaaccacc aggaagtcgt
     2401 ccagatcctc ctggccgcgg gcgccgaccg ggacatcgcc gatcgcgccg gcgtgtcggc
     2461 gctgcagcac gcggagcaac gcggccagaa cgaggttgcg cgcctcctcc gccaacgctg
     2521 acctgacacc ccgcgcgggt atgccgtcgg gtccggtcgc gcgggccgag taggccgcct
     2581 ttgaggagcg ttaccgacac tcccgtgtgg gtatcactcc gccgagccac ccgtggggtc
     2641 ctcggcgccc caattcccca gagcgacgat cgcctcgcgc agggcaagcc cgcgatcggt
     2701 caacgcgtag gcccgggtgt tgtgccgcag tggcaaccgg gacagcacac cggccgcttc
     2761 gagttcgcgc agccgggtcg cgaggatgtt cgtcggcacg ccgaggtcgc gctgcagatc
     2821 gccatagcgc tgcggcccgt cgagcaggcg atccacgatc agcagagccc accgtgcccc
     2881 gacgacctcg agagcggcgg cgaggctgct cacgccgtcg gatcagcgtc cggcttcatc
     2941 cagaacggcg agtagtgata gccgtcgggg tcatcgaact ggcgctgata catgaagggg
     3001 tagtcgtcgg tgtcaccgac gcgcccacca gcggcagcgg cccggtcgac aatctcgtcg
     3061 accgcctcgc ggctggccag gtcgaaggag accgtgacct tcgagggcgt gtccggccca
     3121 ccgatcaact cctcgacccc gccgacgctc gcatacatct cgcggctgcc gagcatgacg
     3181 tactgctcgg gcgcgatcgc gaagcacgac acgttgtgat cggacatctc ggcgttgagg
     3241 gtccacccga gggcggtata gaaggcagtc gcgcgctcga cgctctcgac cgggcaggtg
     3301 atgaagaggc tcatgtgggc gatacttgca aattacaagt gcgatcgtca accggcagac
     3361 gggagccgtg aggggccgac tcccatccgc tcgctggctc ttaggagacc gtgaagatga
     3421 ccttgccaaa gacgtcaccg ttggccatgt gctcgaagcc ctcccgagcc tccgcgagcg
     3481 gccgcaccga gtcgatgacc ggcgtgatgc ccttgccctg aacgaacgcc gccagccgct
     3541 ccaactcggc cttggtcccc atcgtcgagc cgatgacccg caactgcttg aagaagatct
     3601 tcgtcaactc cgccttggcc ggcgcgtcac ccgaggtcgc ccccgagatg acgatcgccc
     3661 cacccggctt gagcgagttg accgagtgcg accaggtcgc cgcccccacc gtttccatca
     3721 ccgcatcgac ccggtccggc agccgctcgc ccgacccgaa cgcccgatcc gcaccgatct
     3781 caagcgcccg cgcacccttg gcctcgtccc gcgacgtcac ccacatccgc aagccggccg
     3841 cggaccccag ttggatcagc gccgtcgcca cgccaccgcc cgcgccctgc acgagaaccg
     3901 ttgccccagg cgtcaacccg ccgttgacga acaacatccg gtatgccgtc aaccacgccg
     3961 tcgacaggca cgcggcggtc tcccacggca taccctccgg cttggcgacc aggttgcggg
     4021 tggggacggc gacctgctcg gcgagcgtcc ctgggtgcaa ctcagacaac agcgtccgcc
     4081 ccgggtcgag ggtctcgtcg ccggaccagc cgtcgctcgc gacgaccgag tggacgatga
     4141 cctcacgacc cgactcgtcg actcccgctc cgtcgcagcc gaggatcatc ggcagtttgt
     4201 cggccgacag ccccacgccc ataagcgacc acacgtcgtg gtggttgagg ccggcggtgc
     4261 gcaaggtgac caccgaccaa cccggtcgcg cctcgggggc gggtcggtca ccgacaacga
     4321 ggccggagag ggggttggca cggtcctggg aggcggcata cacagcgagc atggccccca
     4381 gcctaggagg cgagatgccg ctgcggcagg gcgagggagg cgtgaggtga ggctcaggct
     4441 gcggactcgc ggcggtcatg cggcagcgtg acgtactgcc gcccggtcgg cgaggtccag
     4501 gtcagcgtgc cgtcccaggc gagatcgacc gaccacccgg cgtgatgttt gaagccgtga
     4561 tgtcgccggc acagcgcgtg caggttgcgg gcatcggtcg gcccctgcgg gaacggcacc
     4621 gcgtggtcga gatcgcagcg cgcggcagcc acccggcacc ccgggaaccg gcaggtgccg
     4681 tcccgctcgc ggaccaggcg ggcgagcgcg gctcccggtc gataactctc cgaggagagg
     4741 tatgccgtgg cgccggtccg cgggtcgagc ttggccaggc gaaccagcgt gtcggggtgg
     4801 cgcaggatcg ccgcgacctg atcgcccagg agccacccac atgcccccgc gtcgacgaca
     4861 cccgacccgg cggcaggctg ggcaccgccg gaaacgacgg tggccggggc ggcgaggccg
     4921 gcttgcgcgg agccggccag cgagtcgtca actcgggtgc cagccgcctg atcggcaggc
     4981 tcgctgaaca ggcgagtggt gagtgcggtg cgagtgaagg gcgggtgcac gtcgggcggc
     5041 aggtagggca gcgcgggcga cgggatcgac gccgcaccaa ccccgacgaa ctgaggctgc
     5101 ctgaacgcct gcccctgctg ccccgacccc tgctgcccgg acccccgctg cccagaccct
     5161 tgaacctgct cattcgcttc cagcaaggcc acgatcgtgg catcggtgac gccgatcgtg
     5221 acgatggtct ggacgtcgac gttgcgcaag agcaggtcga ggaacgcatc ggcgcgggcc
     5281 tggccggggg tgagaccggg cttgccacgc aggtattcat cggccaggtc gcacaccgca
     5341 ctccacgcca gttgggaccg gtcggcggcc gcgatcgcgg tccactccgt ggtccccggc
     5401 aggacccctg gcgcgacccg cacgcaccgc gagcgcttgc cctcggcgac ccgctgctcc
     5461 aacaccccgg cgtcgacctt ggcggcaacc cgctcggccc gcttgcgcag gacgccggcg
     5521 gtcgcgtcgc ccaagccacc ctcgccgaac agcgccacct cgaagtcggg catccgctcc
     5581 gaggccaccc cgaacgagcc cgcgaccacg gcccccaccc gccacggctc caactcaccg
     5641 tcgagagccg cccgcagggt gcgcggcaac tcgttgacca ggacgcgcgc ctcgcccaac
     5701 cgcccggcca tcgtccgcgg cggcagatgc agcaacggag ccagcaccga tggcgtgtag
     5761 tcatcgccgg tgccccaccc cgccgggaag tgcccggcca gccggtcgcc gtccttggca
     5821 agctcgacgt catgctcgac ccggcgggcc accgtctcga tgacgatcgc ctgcaaggcg
     5881 gtcacctggt tgagcgccac ctgcaactgc gcgacgacct cttccgcgac gctctggtcg
     5941 aggtcgagca gggccagcgg gtcgacgcca aggagcgcat cggcggccag caacgggctg
     6001 acatcgcgca gggatcggcc ggccaggacc tccaactggt cctggccgac ctcccacggc
     6061 agcccttcga acatgccccc attctatcga acacccgttc gtacgtcaac catcattcgc
     6121 gccaattaga ttgtccgcca acatctttcg tcacatgcgc cccaccttcc tcaccgctcc
     6181 cccaccctcg tcaaaaggcc cccagcgccc caaacacaac ggcgcgcaat tgccgccacg
     6241 cggcataccg gagcaggtag gttcgagccc atgacgtctc cttccagcgc caccaccccg
     6301 gcgcccaccc gcacccccac cgccgtcgac gccatcgccg agaactacct cgcccggtat
     6361 gccgcgttga gccccatcga ggccacctac ctcgggctgc ccggccacga cgaagacctg
     6421 gacgacctct cccccgcggg ctatgcggcg atgtccgcgc tgcgccgcga gaccctcgcg
     6481 agcctcgccg atgcggtggc cgtcgacgac atcgatctgg tcaccatcgc ctcgatgcgc
     6541 gaccggctcg gggtggccga ggagaagtac gccgccggca tggacgagat gagcctcaac
     6601 gtcatcgcct cgccgctgca gaacgtccgc gacgccttcg acctcatgcc gcaggacacc
     6661 gacgagcact ggcgcacggt cgcgatcagg atggcgaagg tccccacagc gatggcgcag
     6721 tatgccgagt cgctcacgtt ggctcgcagc cgcggcgatg tcgcgccgcg ccgccaagtc
     6781 gatgcctgca tcgtccaggc ccgcgacctc accgcggacg acggctactt cgtcaccctg
     6841 gtcgccgagg ccaaggcggg agacacggcc ctcgagccgg aggtcgccag cgacctggcc
     6901 gcggcggcgg aggccgcgcg ggcggcatac gtgcagatcg ccgacttcct cgagaccgaa
     6961 ctggctccgg tcgccccggc agccgatgcc tgtgggcgcg agcgctatgc gctgcactcg
     7021 cgcgactttg tcggggcggt cgtcgacctc gaggagacct accggtgggg ccaggaggag
     7081 ttggcccgga tcacggccca gatgcaggag gttgccgagc agatcaagcc cggcgccacc
     7141 atcgaggagg ccatcgaggt cctcagcgcc gaccccgcct acctcctcga aggcaccgac
     7201 gcgctgcgca cctggatgca ggggaaggcc gacgaggtca tcaccaacct cgcggacgtc
     7261 cacttcgaca tccccggccc ggtgcgcacg atcgagtgcc gcatcgcgcc gacccagacc
     7321 ggcggcatct actacacggg gccctcggac gacttcaccc ggccgggccg gatgtggtgg
     7381 tcggtgccca agggcgagac gacgttctac acgtggagcg agttgtcgac cgtctatcac
     7441 gagggcgtcc ccgggcatca cctccaggtc gcccagaccg tcttccgccg cgagttgctc
     7501 aacacctggc ggcgcctggc ctcctggacc tccgggcacg gcgaaggttg ggcactctat
     7561 gccgagcggc tcatgtccga gctcggctac ctcgacgacc ccggcaaccg gatggggtgg
     7621 ctggacgcgc agtcgctgcg tgcggcgcgg gtcgtcatcg acatcggcgt gcactgcggc
     7681 ttcgaggcac cggcggaggt cggcggcggc gaatggacct atgacaaggc ctgggccttc
     7741 ctcaccgccc acgcccacga gtccgagggc aaactgcgtt tcgagttgga ccgttacctc
     7801 ggctggccgg ggcaggcgcc gtcctacaag atcggtgagc gactgtggct gtcgctgcgc
     7861 gagcaggtgc gcgagcgcga cggtgcggac ttcgacgtca aggccttcca ccgcaaagcc
     7921 ctcgacctgg gcggcgtcag cctcgacacc ttgcgtgacg cggtcctggg caacctaggc
     7981 tgacccccgt cgccggccgg gcggcatacc cgagcgtgtg aaggattcgc cccgtgaccc
     8041 ttgccatcgc tgtggtcgcg ttggtcgcca ccgtgctcac ggtgacggag ctcgcgcggc
     8101 gcctggacgt gtcagcaccc ctgctgctga cgattgtcgg cgtcggcgcg agcttccttc
     8161 cgtttgtccc tccgctcgtc gtgtcgccgg agctggtcct cgtggggttg ctgcccccgc
     8221 tgttgtatgc cgcgtccgtc aagacctcac tggttgacct cggagcgaac aggcgcaaca
     8281 tcgggatgct ctcggttggc ctcgtggcct tcaccgccat cggcgtgggg ctggccctct
     8341 ggtggctctt cgatgtgccg ctgccgctcg gcattgccgt cggcggggtc gtcgcgccgc
     8401 cggacgcggt ggcggcaacg gctgtgggcc gccggatcgg gctgccgcgg cggatcgtca
     8461 ccgtccttga gggcgagtcg ctcttcaacg acgcgacggc gctggtcctc gtgcgactcg
     8521 ggatcctggc actcggcagc gcgttgagcg tcggtgcggt ggtcgggcaa gtcgcctgga
     8581 ccagcatcgg tggcgtgggg atcgggctcg tcgtcgcgca ggtgctcacc tgggtgcgcc
     8641 gccgggtcac tgacccgatc agtggaacgg cgatctcctt cctcgcaccg tggctggcct
     8701 atctccccgc cgaggagttg cacgcgtccg gcgtgctggc cgtggtcgtc gccggtgtgc
     8761 tcctcggcca ccgggccccc gtcatccagt cggccacctc gcgggtggcc gaccggatca
     8821 attgggcgac agcccagttc atcctggaga acggcgtctt cctgctcatc gggctgcagg
     8881 ccaaggggat catcgaacgg atcacggcga cacctgctgg ggtcagccgt gccctgttca
     8941 tgtccgttgt cgtcctggct gcggtggtgg tgctgcgacc gctctgggtg atcccgctgc
     9001 gctttctgat gggtgatccg caggaccgcc gcgatcagct ccgctcatcg gtgatcgtgt
     9061 cgtgggcggg catgcgcggc gtcgtgaccc tcgcggccgc cttcaccctg cccgtggggg
     9121 tgcccgaccg ggacgccttc gtgctggccg ccctcgtcgt ggtcgcaggt accctcctcg
     9181 tccaagggtc gtcgttgccc tgggtggctc gtcgcctcga cgtccacggc ccggaccccc
     9241 gtgaggacgt cctgcagacc gccacggtga tgcgcagcgc agtcactgct gggctgcgcg
     9301 agttggaccg gctggccgcc ccgaccgagc cgccgcttcc cgacgggttg gtcgaccagt
     9361 tacgcacgat ggccgagcgc cgggtgcatg ccgtctggga gcagttgggt gcgccgtccg
     9421 ggacagaacg tcccgacggc tcgggtggag ccatggtcga gacccccagc gagagttacc
     9481 ggcgggcgcg gacagccatg ttgcaggctg agcgcaccga ggtcctccgc ctgcgcgacc
     9541 tcggcaccat cgaccatgag gtactcgcag cggtgctggc cagcctcgac gtcgaggagt
     9601 cggtcgtcac gtccgtctcc gagcgacagg gcgccctgcg ggagcggctc gtgctcactc
     9661 ccgagtcgag caagggcgac tgcctgcacc tgcgggatgc ccccgtcggc atcgagccgg
     9721 tcacgccgga cggctgcgct gactgcctgc gcgacggcac cacctgggtc cacctgcggc
     9781 tgtgcctgac ctgcggcaac gtcggctgct gcgactcctc gacgggtcgg cacgccactg
     9841 cccaccatcg tcagacggct catccggtga tgcgcagcat cgagcccggc gagggctggc
     9901 gctggtgcta cgtcgacgag ttgctcggct gagacgtcgt gtggctgagc tgaggcgagc
     9961 cgcaagggac gggagcactc cggatcgttc gaacgccccc agcgtccgcg ccccccgctt
    10021 tgccccacac cggcattccg cctgcccgcc cctcccacgg gcttgacggc gcggcataca
    10081 ttgacgtggt gaccacagcc cgctttcgac tcgtgctcgc gtccgcctcc cccgcccggt
    10141 tgaacaccct gcaacatgcg ggtgtggagc cgaccgtcgt cgtgagtgac gtcgacgagg
    10201 acgccgctgt ggccgaggcg ctcgagcgct acggcccctt ggagcccgcg caggtcgcgc
    10261 tcgtccttgc ccgcgccaag gccgaggcgg tcgccttcgg tgagcgcgtg ggtgacctcg
    10321 tgctgggatg cgactcggtc ctcgaattcg acggacaaat gcacggcaaa cccgccgatg
    10381 ccgacgaggc gagggccagg tggcgccgga tgcgtgggcg gcatggtgtg ctgcacaccg
    10441 gtcattggct cgttgaccag cgtcccgtcg agcggggcgg cagccaagcc accctggggg
    10501 cgaccgcatc gaccaaggtc tacttcgcga aactcaccga cgaggagatc gacgcctacg
    10561 tcgccacggg cgagccgctg cgggtggccg gcgctttcac catcgacggg ctcggcggcc
    10621 cgttcgtcac cgggctggag ggtgaccacc acaacgtcgt cgggatctcg ctgccgctgc
    10681 tgcgcgagtt gctcatcgac atcggggtca actggttcga cgtcgtcacc gtccaccagc
    10741 gctgacgctc gacgcgcgca ggaagacttc agcagtccct aaggggcctg gtcctgtgga
    10801 gctggcacaa ccgggatggg ctgggcccgg aaccacgctg atgatgctgg cagccacagg
    10861 agtgcgactc cgaccaggcc cagagctgag gcggccgcgt agctcaccgt gaagacaccc
    10921 agtcccccaa tcaccgcgaa caacgtagcc actcttctgg cccatgtgga tcgacggctg
    10981 aggcccagga tgaccaagag gtagctcgcg acgccaaccg cagcggccac aaacgcggct
    11041 ttctggacga cccctccggc cctctcgtcg gcgacggaga ccaccatgct cagcgaggcg
    11101 tttgcaatcg gcagcccggc cccagaccag tagatcgtcg ctgacgcacc ggcgactgcg
    11161 gccgcgccga tgagactgag cgcccagatg actgcccgcg gaggtcgagg cttgcccgcc
    11221 cggttaactc gaacgggttc ggccgcgaat tcgatgctgc gcaggatccc gtcggcactg
    11281 tgagcgaggg aggtgtcgac cactcctttg accgcatcct tgccacgggc gagcgctggg
    11341 ccggccgact tcgctttggc ctgagccgat gacaatcctt gagccgccac aggcgtcgca
    11401 gctcgaaccc ctgccgcccc gagctctcgg cccttgacgg cagcgcctcg gagcccccgc
    11461 agcccccgtg ccagatgggg cgcggcttcc gacactgcgc cccggccgag tccgacgcca
    11521 cgcccgacga ggctttgagt gaccggagcc gggtgagggg gcggcggcgg aggcgccggc
    11581 gggtgggccc ccgtccctgc gtccgcatcc tgcggcgcat gcccgccagg acttctctca
    11641 tctggcaggt tcggtccgtc ggtgacgggc atagttggcc tccctggctc ggtctcgcca
    11701 gtccctcctg acggacagac gggacgctca ggggaactgc acgtccgggt agaggccgag
    11761 gatcacccag tagttccaca cccaccaacc ctgcagggcc acgagcgcga gcgcgtatgc
    11821 cgtgagccac cacgcccgcc gtggccgcag ccgcggacgg ttgaggatcc aggcaagcag
    11881 gaggaacacc ggccaggcca acagcgcgta gcggatccgg gatggcccga tgccggtgac
    11941 caacaccatg tatgccgggt aggcccccgc ccagccccac agctcggggc cccaccgcgc
    12001 agtgtcgacc gagagcatct tccacgcgaa ccacccgatg cccgcaaggc acgcggccca
    12061 cccgaggtag ccgtagtcgt agtagatgta gtcgacccag gtgtgcagct tgatctcgaa
    12121 cgcgtcgacc ccccaggcgc ccatcgtctt ggcgtagccg tccttgtcac cggtgacgag
    12181 gcggacgatc gtgggccaga ggaaggtcag ggcacccgag agcagggtga gcacaacaag
    12241 cccgacgcgt tgccgcaccg ggaacgggtc ggcgtcgcgc cgctgccaac gcaccgccgt
    12301 atgagcgatg agcggaggcg ccgagatgag gacgaggttg cgggtgagcg ccagcgccac
    12361 gaggaggacg agcacccacc agtagcgccg accgcgcaag gccgtgagca gcaggacgat
    12421 gactagcaga gcgcaggcct cggagtagtc ggtctgcagg accggtgccg cgacccacgt
    12481 cgacgtcgcg acggtcgcga cgatcgccga ccaacggccc gcggcacggt cgacgagacg
    12541 gaacatcagc acgacggcga tcgcgccgac gaccgtcgac aacagggcgc cagcgacgta
    12601 gaagttcgcc ccggtcgccc gcatgacgag ccgggagagc atggggaaca tcgggtagaa
    12661 ggcccacggg ttctgcacga ccttgccggt gaccggatcg gtggggatcg ggtcgggata
    12721 ccccaactcg gcgatctcgt ggtaccactg cccgtcccag ttggccatga gcggccagta
    12781 ccccggcgcg gcaggcgtgg ggaagatgac ccgcgcggcg ccgtttcccg gcaccatcgg
    12841 gatctgcgag cgctgcatga tcgcgaagta gacggcgtcg atgagccggg tcacggcata
    12901 caccgcgagt gggaaccaca ggggaaagcg gcgccaccac ggcgtcccgg gagcgtccgc
    12961 ggtgcgcggc acggcctcct caggcgggat cggtgccgtg aaggcgccag ccactcgcgc
    13021 gccccaccct ggtcccatgg tgggcaatgg tagaggggta tgccgccgcc ggtcaccgcc
    13081 cgggcgaccg acctcagaca ggcgggacgg aacggcggcg ttcgctgaag tgccgatccc
    13141 gtcgggaggc gtagcgcagc cggtggagca actcctcgcg cagggcatcg gcctcgatga
    13201 ccccgtcgac gacgaggtcg gcggccaggc gttcgaggtc gacgtcctcc tcgtattcgg
    13261 cccgtcgctc cgcgatgtag gcggcccgct cgtcggggtc ggcgatgctg gcgatcttgt
    13321 tggcgtagac ggcgttgacg gccgcctccg gccccatgac ggcgatcttg gctgtgggca
    13381 atgcaagggt ggcatcgggg gcgaaaccag gcccgcccat ggcgtaaagc cctgcgccat
    13441 aagcctttcg gacgacgatg cagaactgcg gcactgtcgc ctcggagacg gccgacacca
    13501 tcttggcgcc atgacggatg atcccgccgc gctcgacctc ggagccgatc atgaagcctg
    13561 ggacgtcggc gagatagagg agcggcacgt tgaaggcgtc gcacaaccag atgaaccggg
    13621 tggccttgtc agcgctgtcg gtgaacagca cgccgccctt gacggcggag ttgttggcca
    13681 cgatgccgac ggtctcgccg ttcatccgac cgaagccgac caccagctcg gcagcgaaga
    13741 gcggcttgat cgggtagaac gagtcggcgt cgacgagccc gtcgatgacc gtgtggatgt
    13801 cgaacggctg gctctcccgc tcaggcacgg tatgccgcgt gagcggctcc gagggctcct
    13861 ccggggtgta ggtgggcggg cgttcgcgcc aacagccggg gacgtaggag aagtagagct
    13921 tggccagctc gatggcctcc tggtcgtcgt gtgccagcag atcgcccacg cccgagacgg
    13981 tgcagtgcat ccgcgcgccg cccatctcct cgagggtgac cttctcaccg acgaccatct
    14041 cggccatccg agggctgccg agatacatcg aggcgttgcc ctcgaccatg atgatgaggt
    14101 cgcagaagct cgggatgtag gccccacccg cggctgaggg gccgaacagg cagcagatct
    14161 ggggcacctt gccggacagc gccacctggt tgtggaagat ccggccggcg ccgcgccgcc
    14221 cggggaacat ctcgacctgg tcggtgatcc gggcaccggc cgagtcgacg aaccagaaga
    14281 ccggcaactc ctcgcgcatc gccatctcgg tggcgcggac gatcttctcg acggtccggg
    14341 ctccccacga gccggccttg acggtcgggt cgttggcgat gacgatcgcg gggcgcccgt
    14401 cgaccgtccc ccgaccagtg acgacgccgt cggcaggtag gtcgccggcg agggcgttgg
    14461 catagcgccc gtcctcgacg aaggacccgt catcgaacag cagcgcgatc cggtcgcgga
    14521 cgtagagctt gttctgttgt acgagcttgg ccccgaggct ctcggtgagg ggggcggagg
    14581 tcgccaggta tgccgccgcg aaccgctccc ggacgctgtc gacccgaggg tcgccaccct
    14641 cggacgcgga cggaccggcg aagggggctt ctgtcgggtc ggtgctcggc ggcatacctc
    14701 atcctctccc agggggaacg cgtcggtgac gtgacgtgcg ctgatcctca ggcgggcaga
    14761 ccgaggcctc gggcgatgag catccgctgg acctccgagg tgccctcgcc gatctcaagg
    14821 atcttggcgt cgcgatagaa ccgcgccacc gggtattcct ccatgaagcc gttgccgccg
    14881 aacacctgtg tggcgatccg agtcgcggtg accgccgcct cgctcgtgta gagcttcgcg
    14941 atggacgcgg cctgcttgac ctcttgcatg gtgcgtcgcc cggcgtccat ctcgtctttg
    15001 agccaggccg ccttgtaggt cagcaggcgg ctcgtctcgg ccaggaccgc gaggtcggag
    15061 acctggaagg ccacgccctg gtaggtcccg atcggcttgc cgaaggccaa ccgcgtcttg
    15121 ctgtatgccg tggcctcctc aagcatccgc cgcgtgcaac ccgtcgcgag ggcggcgatg
    15181 gcaatccgtc cgtcatcgag ggtggcgagg aactgcgcga agcccttgcc ccgctgaccg
    15241 agcaggttgg cctcgggcac ctgcacgtcc tggaaggaca ggccgtgggt gtcggagatg
    15301 tgccacccaa gcttgtggta gggcggagcg atatcgaatc cctttgtgcc ggaagggatc
    15361 atgatcgcgc tgatctcggg cgtcccgtcg ttgcgggtgc cggtgcgggc cgtgacgctg
    15421 acgaccgaag tgatcggcga gccggagttg gtgatgaacg acttggcccc gttaaccgtc
    15481 cagatgccgt cggcgagggt cgccttggtc ttcgtgccgc ccgcgtccga gccggcgtcc
    15541 ggctcggtga ggccgaaccc ggcgagggcc cgtccggcgg caaggtcggg caaccagtgg
    15601 gccttctgct caggggtgcc gtagttgagg atcgggttga tgccgagccc gaccccggct
    15661 tcgagggtga tgccgatggc ttgatcgacc cggccgagtt cctcgatcgc gacgcacagc
    15721 atggtgaagt cgccgccgct gacctcgggg ccggcgccct cggggcggcg ggtgccgccg
    15781 aactcctccg gcaccaccag gccgaacagg ccgagctcac cgagtttggg gatgagctca
    15841 gcggggaagt gcgagtcctt gtcccactgg gcgacccggg gctcgatctc ggcctcggcg
    15901 aactcccgca ctagagcccg gaactcctcg tggtcggcgc tcagctcgaa catggctgcc
    15961 tcacttccgt gccgcaaccc gcagcgtcgg tgacgggcgt ggcgttggcg gggacgcggc
    16021 ataccgccat ccctagttga cgttaacgtc aacgtaaagc atgctgaccc ccatggcaag
    16081 cggacgggac ggcgagcgga cgtggtctat cgcacaggtg gcggccgagt tcggggtcac
    16141 ccatcggacg gtgcggcact acgaggacat cggcctcatc gcccccgagc gacatggcac
    16201 ccggcgggtc ttccgccgcc gcgaccggac ccggctggcg ctcatcctgc gcggcaagcg
    16261 gctgggcttc ccgctcgagg agattcgcac gatcatcgac ctctacgatg cgccgcgcgg
    16321 caagaccagc cagttggaat acgccatcac ccaggcgcgc aaacggcggg tcgaccttga
    16381 ggaacgccgt cgggacatcg acgaatcgct agccgaattg ggcgcgttcg aggcgtcgtg
    16441 ccaggccgat ctcgaccggc tcagctgaag gccagcgagg gcggcgccgc ggctgccgca
    16501 cccggttgac aaccggaagg agcgcgcgga agggtggtct ctccggcggg gcatccgcgc
    16561 ggcacactcg ggggcaggaa agggcacatc gatgggcttc ttcaacaacg ccaaacttga
    16621 accgacacca gaactggcgc cattgaccaa ggagcgcatc aaagcaaccc tcgaagccag
    16681 cagttggtcg tatcacgtcg actccgacgg tgacatcggc agcggctggg atcacggggt
    16741 catttggttc ttcacctacg gcaaccaggc cgagatcctg caggttcgag ggatctggga
    16801 cggcaagctc agcccggacg acctgtcgca ggccctcgac gtcgtcaacg aatggaactg
    16861 cgaccggctg ttccccaagg tctatgcccg gatcaacgac gagggccagc tccgggtgat
    16921 ctctgagcac accttggact acgagcacgg gctgaccgac gagcagctca acctgcacgt
    16981 caacacgggg atcgccacga gctgtcagct ctgggaacgg ctcaacgagg ccttccccga
    17041 ggcggctgcg gctgccgccg cggaatgacg ccgacggccg cccgctccgg cgctgcggcg
    17101 cacggagcct gatgtcgcag cgcttccggg tcgacctggg tggcatggtc gacctgctgt
    17161 cccaccacct ctactcgggt ccgcatgtct acctgaggga gttgctgcag aacgccgtcg
    17221 acgccgtgac cgcgaggttg gccgaggatc ccgcagcccc ggcaacgatc cggctcacct
    17281 cggacaccga ggccgacgga gcgacggttc tcgtggtcac cgacagcggg gtcggactca
    17341 cctccgacga agccaccgag ttgctcgcga cgatcggccg ctcctcgaaa cgggatccgc
    17401 acggcctggg ggtggcgcac tacctcggcc agttcgggat cggcatgctc acggcgttca
    17461 tggtcgccga ccgcatcgag gtcacgtccc ggtcggcccg acccggcacc gtccccaccg
    17521 tctgggtggg gcgcagcgac ggcaccttcg aggtgtcgga cctgccgccg gggccgtccg
    17581 cggacgcgac ggcagtgggc accaccgtcc ggctgtgggc tcgacgcggc agcgagcatt
    17641 ggctcgaaac ctccaccgtc gtcgctctgg ccacggacta cggctcgctg ctgcccttcg
    17701 atgtcgccgt ccgcgtgccg gtggagggct cggatcccac gtggcggcgg atcaccgacc
    17761 cggtcctccc gtggcaggcc cgccacccgt cggcggaggc ccgctcgcgg gcgctgacgg
    17821 catactgcga acgcgtcctg ggcttcaccc cgctgggaca catcgacctg agcgtccccc
    17881 tcgccggcgt gtccggggtg gcgttcatcc tcccccaagc cgtgacgccg ggctcgggac
    17941 ggcaccgcgt ctacagcaag cggatgctcg tcggggcgcg cgtcgacggc gtcctgcccg
    18001 actgggcctt cttcacccgg gcggtcctcg actcggacgc gctctccccc accgcgtccc
    18061 gcgaacagat ccacgacgac gagatcctcc tggcgacccg tgacgccttg ggggcgcaac
    18121 tcaaggagtg ggccaccgcc actctgacga cgccctcgac cctgtcacgg cggttcgtcg
    18181 agacccacca tctggccctg cgctcgctcg ccctgaccga cgacgagatg ctcgacctcg
    18241 ccgcccgggt cctgccgttc gagaccaccg atggcgcacg aaccttgcgc gacgtcgcgg
    18301 aggacggcga gatcgtgttc acgtcaacga ccgaggcgtt ccgacgggtc gctgcggtcg
    18361 cccgcgccca gggcctggtc gtcgtcaacg cgggctacgt ctacgacagc gacctgctga
    18421 cccgactcgg gcggcgccca ggctgggcgg tccgcgagtt gtcgagcgag gacgtcaccc
    18481 aggtcctgac accggtggca accgaccgcg ccgggcagct cgcccaggcc ctgtcgatgg
    18541 ctgaaaaggt cctcgaggac gaggactgtc acgtcctcgt gcgcacgttc gaccccgatt
    18601 cgctgcccgc cgtgctgctg cgcgaccctg acggcgagca tcgacgggcc ctggcccgtg
    18661 agcgggaggc cacgccgggc ctctggggcg ggctcctcga ctccttcgag accgacacct
    18721 cgacgcggac gcgcagcctc gtgctcaacg actcggcccc cgtcgtgcgg caactgcttg
    18781 cggcaccaga gaccatcgtg ttcgccgcgg gactgcgctc gctctatctc tcggccgtca
    18841 tgctcgccgg cgaaggtctt cgatcgagcg aggtggccgg cctgcacgac gcgctgagcg
    18901 tgctgctggc ccgatccctc gaccacgact gacctacacc ccgccgcact gaccacgtct
    18961 gccctgacca cgtctgcact gaccacgccc gagaggacac ctcagatggc accaagcgac
    19021 gccgaggtcc ggcgcgaact ccgcgagatc cgggccatgc cctacggcag cgcccgcacc
    19081 gccgccgcgg aggtcatcgt ccggcgcatc gatgccgaag gccctcagga gcgtctggcc
    19141 gaggcgttgc tggatctcgt cgaggcctac agcttcaccg gtcaggggca ccgatcgttc
    19201 gtcgccttcg cccggttgtt gcggctctgg gacagccaac ccgaagtgtt cgacgagtcc
    19261 gacgagcaca acctgttctg ggagttcaaa tgggtcgccg gcgatctgcc cgacttcccc
    19321 gagatcaccc gcgagcaggc cgacgccttc ctcgccgaca tgagacgccg cttcgacctc
    19381 gccggcaagg gcagcgggtc ggtgctgggg tcgcggttcc gctgggcctg gcacagtggc
    19441 gacccgagca tggaggcggc gcggctggcg tggctggcgc tgcccaagga cgacatggac
    19501 agttgcgccg cgtgccggat cgggcaccag gtcgactacc tgaccgagac cgggcgcttc
    19561 gaggaggcga tcgagctggg tctcacccag cactcgagct gcaacctgga gcccacccga
    19621 accctgcacg ccctggccct ggcccacctc aacgtcggcg cggacgggcc ggcgctcgcg
    19681 gcataccgta gggccctggc cacgctgaac acctccgaca gcgactacgc gccggcccgc
    19741 ggacagggct tcgagatggt ggcgcggggc ggtcacgtcg agcgggccct gcgcttcctg
    19801 cgtgaggacc accccgagtt gctcgaaggc gcatcgacac ccttgttccg gttgcggttc
    19861 ctgttgggcg tgctcgccgg gctctcggcc aacctcgacc acagcgacct gccgaccggg
    19921 ctgcgctccc ctgacgcccc gaccctcggg cagttgcacg cttgggttca cgagcagacc
    19981 gctgagttgg ccggtcgctt cgacgctcgc ggcgggagcg actactacgc ccggctgctg
    20041 gaccgttcgt tggccgccac ccgcacgccg gtgcccttgg acttcgacat ctcggccatg
    20101 gcgtccctcg gcccgcagcc gggggccggc gacccgacgc accagcccga cgccgacccc
    20161 gccccggcag ccctcggggc agacggcgtg gccgccgcgc tgacgatcgc cgagcgccac
    20221 gcacgggcac gctcctaccc cgaggcggcc gcggcatacc tgcgggccgc cgaggtggcc
    20281 actgcggcgg ggctgctcga acacgcgggg atggcgtggg cggagtctgc gcagtgcgcc
    20341 gcccaggtgg gtgacgaggt gaccgcccat gccaggtatg ccgccgcact ccccctgctg
    20401 cgcgcggccg acggcgaccc cggcgcgatg gcgcagatcc tcgctgcgtg ggcgcccgtg
    20461 gccgccgggc tcggcgacac cgacgccctc atgacccacc tcgacaccct cctgtcgtcc
    20521 gacggttccc acgaagccct cctcgacagc gacctggagg agcgtcagcg ccgtcagtcc
    20581 gagaacctcc aggcctcgct gctggagacg tgggcgcgcg ttgtcgcgtc gcgcagtccg
    20641 ggggtcgacg ggcagatcga cgggcaggtc gcggggcagt cggcccgcga tgccgccgac
    20701 gcggccaccc gcgccggcga gttgttcgcc caggtcggcc ggatcgggtc ggcagcacag
    20761 gccttctggc tcgccggcca gatcctgcgt gaggacggcg acctcgacgg ggcggtcctt
    20821 gcgctcgagt cggcctacga ggggctcacg atcgcccgcg acgccgccaa ccgggcccgg
    20881 gcggccagcg acctcatcga cgtgttgcgg gccactggac agcacgatcg ggccgatcag
    20941 gtgatcgcca cgctgacgag cggctcgtga cccggcccgc tgtcaggcca atcgccggct
    21001 gtagggccaa cggacacccg gcgcaacgtg agccatccct ccactgccgc tccggtggtg
    21061 cggcacgatc cgagcgctag gctccccccc atggcgaaga tctcaaaggt gctgatcgca
    21121 aaccgcggcg aaatcgccgt gcgcatcgcc cgggcatgca aggacgctgg catcggatcc
    21181 gtggcggttt acgccgatcc cgatcgcgac gccctgcacg tcaaggtggc cgacgaggcg
    21241 tatgcgctca acggctccac acccggtgac agttacctcg tgcaggagaa gttgctggcc
    21301 atcgccaagg aggccggggc cgacgcggtc caccccggct acggcttcct cgccgagaac
    21361 gccgggttcg cccaggcggt catcgacgcc ggcctgatct ggatcggccc cggcccggcg
    21421 gccatcgaca gcctcggcga caaggtcaag gcccggcaca tcgccctcaa ggccaacgcc
    21481 cccctcgtgc ccggcacgtc cgacccggtg accgggcccg acgaggtcgt ggagttcgcc
    21541 aaggcgcacg ggctgcccgt ggcgatcaag gcggcctacg gcggcggcgg gcgcggcctc
    21601 aaggtggccc gcgagcttga ggagatcccc cacctctacg aatccgcggt tcgcgaggcg
    21661 gtcaccgcgt tcggacgtgg cgagtgcttc gtcgagcgct tcctcgacca cccgcgtcac
    21721 gtcgaaaccc agtgtctggc agaccagcac ggcaacgtgg tcgtcgtgtc gacccgtgac
    21781 tgctcgctgc agcggcgcaa ccagaagctc gtcgaagagg cgcccgcgcc gttcctcacc
    21841 gaggcccagc acgacgagct cgtccgagcc tccaaggcga tcctcaccga ggccggctac
    21901 gtcggcgcag gcacgtgcga gttcctcgtc gggcccgccg gtgacatctc cttcctcgaa
    21961 gtcaacaccc gcctgcaggt cgagcacccg gtcaccgaag aggtgaccgg catcgacctg
    22021 gtgcgtgagc agttccgcat cgccgagggc cagtcgctgg actaccccga cccggtgccg
    22081 cacgcccact cgatcgagtt ccgaatcaac ggcgaggacg ccggtcgcgg cttcctgccg
    22141 gcacccggca ccgtcacggt ctacaagacc ccgtccggcc cgggcgtccg cgtcgactcc
    22201 gggctcgtcg aaggcgacac catcggtggg gccttcgact cgatgcttgc caagctcatc
    22261 gtcaccggcc gcgaccgcca gcaggccctg gagcggtccc gtcgcgccct ggccgagttc
    22321 gaggtcgagg ggatgcccac ggtcatcccg ttccaccgtg cggtcgtcgc cgaccctgcc
    22381 ttcgccccgg ccgaccctga cgaggcgttc tccatccaca cccgctggat cgagaccgag
    22441 ttcaacaaca ccatccctgc gtatgccggt gggccggcgt cgctgcccga ggccgaggag
    22501 cgccagaagc tcgtcgtcga ggtcggcggc aagcggatcg aagtcagcct tccgggaggc
    22561 ctgtcgctcg gcggtggaac cggcggcagt ggcagcggag cgacgaggaa ggcggcgcct
    22621 aagcgcagcg gcgcagccaa gtccggcgcc gcagcatccg gtgactcggt gaccgccccc
    22681 atgcagggca ccatcgtcaa ggtcgcggtc gaagaaggtc agacggtcga gtcgggcgcg
    22741 atcgtcgtcg tcctcgaggc gatgaagatg gagcagccga tcaccgcaca caaggccggg
    22801 gtcatcacct cgctcaaggc caccgtcggc gagaccgtcg ccagcggcac ggtgctcgct
    22861 gagatcaagg acgccgaagg ctgatgccca cggagggctt ctccttcgtc aacctggcca
    22921 tcctcgcggt ggtcgcgctg atcgttgtgc ccggctatcg gcacctgcgc ggcaccgtgt
    22981 ctgcacgtcg tcgcgagcgc tgggcgcagg aggaggctga ctaccgcgca gcggtcgaga
    23041 atgaggaccc cggccaggac caccctgcgg gtgcctgatt caccgccgaa gcctcagggg
    23101 atggtcacag ggccgatgaa accgctgccc ggggcggctg tcgaacaatc gggcacccgg
    23161 cgcaatgcgt atggaccggc ctcagcaacg actttgaagt ctgggtcgta cgccgccgag
    23221 tcgaccccgg agtactccgg ggtgtggtcc agcacccccc agatgtgcat gtctccaccc
    23281 gtcagcacgt agtcgaccct ttgacgacgt gatgcggcac agacgtcggg catgcttgtg
    23341 actgcttgga gcgacgttcc aagcagtcgc cgatcgggca gttggaggga gttctcggtc
    23401 ggatagagca cctcggtgcc ccccgcgacg tagaagaaca tcccgccgcg ataggggttg
    23461 gcggcgacca ccgaaccgct cgggacgaag gccgccagct gggacagggc ggcatactcc
    23521 cgcgggagca cccacgaata gtccgcggag tcctccccgt aggtggcctc cacgacctca
    23581 cgatgcaccg gtatgccgca acccaatgcg gcgacgactg cgacggcggc gagggcgatc
    23641 agcccctgac gcacgcgggg cggatctggt cgtcgtcggg ccacccatcc gccgatcacc
    23701 accgcaccgg ccgtcgcgca gaggactgcg ggcagcacca gggcggcccg gacccggtag
    23761 tggtcgttcc accagagcca ggtgagctgt cggaccaccg ggccgttgac ggcgtacagc
    23821 aacaggaaga tggcgatgaa aagggcgagc gagacgacca gccagcggcg gttccggtct
    23881 cgggcaagcc agaccgcgcc gacgaggacc agcacgacca cggagccgga ctcgagccag
    23941 gagccatcga agagggtcgc ggcctggctg cccgccaacg cccacgtggc acgcacatga
    24001 gagttgatga catacatctt cgtcgggatg aaccgggttg aggccatgac cgctgcggca
    24061 accatgagga ggaaccacgg cagtcgcacg aatcgggccc cccaacggcc gcccaacgca
    24121 tggcgccact gcggaagcgc tgccgcgacg acggtgaggc cgatgacgac gcccgaggcg
    24181 acggcgttgg gctgggtccc cagcagcacg agaaggccaa gggcggcata cccgacggca
    24241 cggacccgac ccgacgcgaa ggcgcgctcg gcgagcatcg cgctcggggc caccgcacac
    24301 aaagcgccgg cgaggacggc gggcaggagc gcgttggcga gcaggttcga ccacaacggc
    24361 ccccaggcga ggaagcgtgt cgggaacgac gtgaagacca gcgtgagcag cccagccgcg
    24421 acgaggatgc cagtgccgcg gccgagcacg actcgggcca ggagcatgac gccgaacggc
    24481 cagacgaggc cgacgaccac gatgagcagc gcgtgccagg tcgccgtgac gggcaacccg
    24541 gtccacgcga ccgtcgcggc ggtgaccgcg tggaaggccc ctgggtaggt cagcgggctg
    24601 tcgagttggg cgtactggta ggtgtcgagg gccgaggcag acccgtgccg caccatccat
    24661 tcgaccgcgc cgaggtggaa gatgacgtcc ggcatctgcg ggatcgccat cggcgagggt
    24721 gcgccgcctg ccaggacgag gtatgccgcc gcgagactca ccgcgcccac cgccaggacc
    24781 gcgcgcaggg gcgccgcctc cgctgcgctg gctcgggcgg cgggccaccg agatcggatc
    24841 gcccgggtcg cgcccaggcc cagagcgacg acgagcacga ccgcaatcaa gcccgtgacg
    24901 gccgaccagc cgacgccgac ggcacgcacc ccgagcccgg cagcgatgat cacggcaacg
    24961 ctgacggccg gagcagcgag caccgcggtg agccaaccga tgccgaccga acgcagcaga
    25021 acggctccgg gtacgaagaa caccgcgaca gccaccagga ccgccggcag caattgcggc
    25081 caggacatcg tcacgagttc tgaactcagc tcatccgggc cgcgacatgc agccgccgcg
    25141 cggcctcgcc gatcgagccg gtcatcgagg gatagacggt gaaggcgttg gacagttggt
    25201 cggcggtcag gccattctcg acggcgatcg tcagcgggta gatcaactcc gacgctcgcg
    25261 gcgcgacgac caccccgccg agcaccgtgc cgtgtccaga ccgggcgaag atcttgacga
    25321 atccgtcgtg gaagccgtcc atcttggccc gagcgttggt cgtcaacggc agtgtgatga
    25381 cgatcgcgtc gactgtcccc gcgtcgacgt cctgctggga caccccgacc gtcgcgatct
    25441 cggggtcggt gaagacgttg gccgacacga cgtccagccg cagcggggcg acggcgtcgc
    25501 cgagcgcgtg ccacatggcg atccggcctt gcatcgcggc cacggacgcg aggggcagca
    25561 ccccggtgca gtcgcccgcg gcatacaccc cgcgaactga cgtgcgcgag accccgtcga
    25621 cgaggacgtg cccgcccgga ccgacctcca caccgacgtc ctcgaggccg agccccgcgg
    25681 tctgcgggac ggacccgacg gccagcaggg cgtggctgcc ttcgacggtg cgaccgtctt
    25741 cgaggcgcac gatgacaccg tcggccgtgc gctcgacggc gctcatccgg gatcggccga
    25801 ggacctccat gccctcgcgg cggaagacct cctcgatgac ggcggcggcg tcgctgtcct
    25861 cacccggcag gacctggtgg cgggaggaca ccagcgtcac ggcgctgccg agacccaggt
    25921 aggcctgcgc gagctcggct ccggtgacac ccgacccgac gacgatgagc cgttccggga
    25981 tctcggtgag gccccagatc tgggcccacg tgaggatgcg atcgccgtcc ggcagcgccg
    26041 tcggcatgac ccgcggggtc gctccggtgg cgatgaggac aacatcggcg accaactctt
    26101 cggtgccgcc gtcgaccaac gagaccagca cccgatcggc cgcgaggagc cgaccggtgc
    26161 cacggcacac ccgcacgccg tcggcttcca accgcgcgtg gatgtcggcg ctctgagcgg
    26221 ccgccaggtc gaggatgcgc tcgttgaccg ggcgcaggtc ggtggcccga ggccgctggg
    26281 gcaggtcgat cccgtgctcg cgcgcggcac ccatccggcg caggacgtgc gctgtcgaga
    26341 tgaggctctt gctcgggacg cagtcggtga ggacgcagga cccgccgaca ccgtcgcgat
    26401 cgacgaccgt cacggaggcc cccaaccgtg cggcgacgag agctgcctcg tagccgccgg
    26461 gaccaccgcc gacgatgacg accgaccacg tgcgcgattt cacccgccca tcaaaccacc
    26521 tgctcacgcg ccctcgtgca cacccgccgt accccccagg tgagccgacc cgttcaggca
    26581 cccgtgaccg aatcgaaatc cgccggggcc tcggcggtca tgccgccggg caaggatggg
    26641 agggcagttc gccatggagt ttcggctccc ctcacggttg gaaggttccc cgagtgagaa
    26701 gcacctcgat caggtatgcc gcgttgtccg ttgccgctct cgctgccgtc ggcatcgccg
    26761 cctgctcccc cccgccgtcc tcgacgtcga cgagcaccac cgccgcgggc ggtggcgtga
    26821 acgccgccaa ggccacctcg ctcgccgact tcggcgggat ggacgggctg gtcgcggccg
    26881 ccaagaagga aggcgcgctc aacgtcatcg cgttgccgcc ggactgggcc aactacggcg
    26941 aggccatcaa gggcttcacc gcgaagtacg gcatccccat cacgtccgac cagccggatg
    27001 gctcgagcgc ggacgagatc tccacggcgc aacggctcaa gggccagagc aaggccccgg
    27061 acgtcttcga cctcggctcg gccgtcgcca tggccaacac ggggatgttc gcgccttacc
    27121 aggtggccac ctggaaggac atcccggaca acgccaagga ccccaagggc gtctgggtct
    27181 acgactacgc cggctacatg tcggtcgggt atgactcggc aaaggtgccg gcgccgaccg
    27241 cgctggccga cctcctcaag gccgaataca agggcaaggt cgcgctcaac ggtgacccga
    27301 ccaaggccgg cgcggctttc gcaggtgtcc aaatggtctc catcggccaa ggcggcacgg
    27361 cggccgacat cgccaagggt gttgacttct tcaagtcgct caaggacgcc ggcaacttcc
    27421 tgcccctgga tcccaacgag gccagcgtcc tgtccggcca gacaccagtc gtcatcgact
    27481 gggcctacaa caacgcgccg ctgaccaaga agctgccgac ctggaagacg tggatcccgg
    27541 acaacgcggt cctcgccggc tactacatcc aggccatcaa cgccgacgcc ccgcacccgg
    27601 ctgctgcccg gttgtggcag gagtacctct actccgacga gggccagaac atctggctca
    27661 agggagccgc cttcccggtg cgctatgacg ccatgaaggc cgcgggcaag atcgacgccg
    27721 ctgctcaggc tgccctgccc gccgtcaacg gcaaggtcgt cattccgacg acggccgaca
    27781 acgacgcagc caaggcctat ctggccgcca actggaccaa ggctgttggc tgacccacga
    27841 cgcctgaggg cgcctggcgg cttgcggccg tcgggcgccc tcgtgggcgt ggccccgttc
    27901 ttcgtctaca cgacggtctt cctgctcatc ccgacgatcg tcgtcgtcgt gggcgcgttc
    27961 gccgacgggt cgggtcgacc gacgctggac aacgttgctg cgctgggcgg ggcgaactcc
    28021 agcttcctgc ctgccctgca gcactccgtc gtcctgtccc tcgtcaccgc gctcgctggg
    28081 gcggtgatcg gggccgtcct tgcgtatgcc gtgtccacgg cgcgccccga cggcctgctc
    28141 aagcggttca tcacctcgtt gtgtggtgtg ctcgcccagt tcggcggcgt ggcgctcgcc
    28201 ttcgccttca tggcgacctt ctcgatccag ggcttcctca ccgtgtggct caacagcctc
    28261 ggcctggaca ccgggtcgag catctggctg tatcaatggg acaagggcct catgctcgtc
    28321 tacctctact tccagatccc tctgatgctc atcgtcttcc tccccgccgt cgaggggatc
    28381 aaaccccagt ggcgagaggc gaccgagact ctcggcggca ccacctggac ctactggacc
    28441 aaggtggcgg ggccgatcct gctgccgtcc ttcatcggcg cgaccctgtt gctcttcacc
    28501 aacgccttca gcgcctatgc caccgcagcg gccctcatca gccaggccag cccgatcgtg
    28561 cctcttcaga tcgccggcac attcacctcc gaggtcatcc tcggccagga gaacgtcggc
    28621 aaggcgatgg cactggccat ggttgtcgtc gtcgccctcg tcatgtgggt ctacgccgcc
    28681 ctggacaagc gcgccagccg gtggctggtc cgatgaggag gcacccgagg tgagcacacg
    28741 gcataccgaa cggcgcaagc gcgccgccct gcggtggacc gtcatcgggc tcaccctggt
    28801 gttcttcctc atccccctct acgggatgct cgagttcacg acccgcgacc tcatcaacgg
    28861 cgggcggacc ggcaagttct ggacgactct cgtcgatgtc ccccaggtga ccgagcgcta
    28921 cccggcgttg gctgagggct tcgcgtcgtc gatggcgctg gcgctcatca ccgtcgtcgt
    28981 catgctcctg ctgctcgtgc cgacgatgac ctgggtgcgg ctgcggctgc cggggctgac
    29041 gaggaccgtc gagttcatct gtctgctccc cctgaccgtg ccggccatcg tcctggtcgt
    29101 ggggctgacc ccggtctatg cctgggtgac ctacctgctc ggcgaaggga ccgtctggct
    29161 ggggctggcc tacgtcatcc tcgtgctccc ctatgcctac cggtcgctgg acgccgggct
    29221 gcgcgcgatt gacgtcaaga ccctgtcgga ggcggcccgg tcgctgggcg cgtcctggct
    29281 gcgggtgatg tggcagatcg tgctgcccaa cctgcgcact gccgtgctgt cggcgtcctt
    29341 cctctcggtc gcgctggtgc tcggcgagtt caccatcgcc aacctcttca gccgcaagaa
    29401 cctgcaggtc gcgatgtttg agctgggcaa gtccgacggc cagttgtcgg tggctgtcgg
    29461 gctcgcgtcg ctcgtgttcg cgttcgtcgt cctgttcgct atgtcgttcg tcggtcgcaa
    29521 ggagaagtca tgacagccac gccctccccc gccgcctcgc gcggcaccga ggtgcggctc
    29581 acggacctca cccgggtgta tcccggcaac gtccgcgcgc tggacgggtt caccttgcac
    29641 atcgcccccg gcgagttcgt cgcattgctc gggccatcgg ggtgtggcaa gacgactgcc
    29701 ctgcggttgt tggccgggct cgaggacgcc gacggaggca ccattgaggt cggcggccgc
    29761 gacgtcacca acgtgccgac cagcaagcgt gacatgggga tggtcttcca ggcctacagc
    29821 ctgttcccgc acatgaccgc gcgacagaac gtcgagttcg gtctgcaact gcgcaagcag
    29881 gacaaggcca cccggcatgg gcgggcggcc gacatgctcg acctcgtcgg gctctccaag
    29941 caggccgatc ggtatgccca tcagatgtcc ggcgggcagc aacagcgagt ggcgctggcc
    30001 cgcgcgttgg cgatcgagcc ccaggtgctg ctgctcgacg agcccctctc ggctctggac
    30061 gccaaggtcc gctcgcagct tcgagacgag atccgccgca tccagcttga ggtcggcacg
    30121 acgacgttgt tcgtcaccca cgaccaggag gaggctctcg ccgtcgccga ccgggtcggg
    30181 gtcatgaatg ctgggcgcct ggagcagttg gccagcccgg cgcagctcta caagaacccc
    30241 gcgacgccgt tcgtcgcgga cttcgtcggg ctgaccaacc gactgcactg ccgcgtcgcg
    30301 ggcggggtcg cccaggtcct cggcggagcc gtgcccctgc tggccggctc agcgggtgac
    30361 ggtttggcca tcgtgctggt ccggcccgag ggcgtgacca ttgcgccggt cggatcgggt
    30421 ggctcggggg gctcgggggg cgctgcggca accgtggtga gtgcgagctt cctgggtgct
    30481 tcgggcaaga tcgtcgcccg cttggagtcg tccgagggcg tcggcgacct ggtgtcggtg
    30541 caggtcgaca acaccgacgt cacggcatac gcccctggtc agcgggtgtt gctcgctcct
    30601 cgtggcgacg cggcgctggc cgtgtcggca acctgacggg ctcggcgcga ggcaccggct
    30661 ggcgcgcgcg gcatacctgt tgcgggagtt gggccctcgc tagagtgccg ggatgaccgc
    30721 gtccctggac ctcgccgacc cgaacaccga tcccttcgag gtggccgccg ccgcagccgc
    30781 ggtgatcgcc gagcggaccg gcgccgagcg gcacgacgtg gcgctcgtcc tcggatcggg
    30841 ctgggggcgc accggtgacc tcatcggtca gaccctggtg accatcgaca acgccgacgt
    30901 gccggggttc cgaaaggccg ctgtcgaggg gcactccggg tcgatgcgct cggtcgccat
    30961 cggcgacacc gggaagcggg ccctggtctt tggcacgcgg acgcacttct acgagggcca
    31021 cggggtgcgg gcggtcgtcc acgccgtgcg cacggcgcgc gccgcggggt gcacgtcgat
    31081 cgtgctgacc aacggctgcg gcgggctcaa ccccgaatgg gggcctggca caccggtgct
    31141 catccgcgac cacctcaacc tcaccgctgc ctcccccatc gagggcgcga acttcgtcga
    31201 cctcaccgac ctgtattccg agcggctgcg cgagttggcc aagcgggtcg accctgggct
    31261 cgatgagggc gtctacgtgc agttccgagg gccccactac gagacgccgg ccgaggtgca
    31321 gatggctcgg atcctcggcg gcgatctggt cggtatgtcg accacgttgg aagccattgc
    31381 cgcccggcag gcggggttgg aggttctcgg gatctcgctg gtcaccaatc tggctgctgg
    31441 gatttccgag tcgccgctgt cccacgagga ggtccttgag gcggggcgcg cggcggcgtc
    31501 gcgatgcggc gcgctgctgg ccaaggtggt caccaagatc gctcaaggga gctgagtccc
    31561 gtgaccgccg attcgactgt gcccgaagcc gacccgacgt tgttcgcggc ggcggaggcc
    31621 tggttggccg atgacccgga cgcctcgacc cgcgcggagc ttgcagcggt gttggaagcg
    31681 gcgcggtcag gggatggtgg tgctggcgat gaggcggccg cggctgcggt cgctgatctg
    31741 gccgaccggt ttgcggggat gttgcagttc ggcacggccg gcctgcgtgg acgcctcggt
    31801 gccggtccga accggatgaa ccgcgcggtg gtcattcggg cggcagccgg gctcacggca
    31861 tacctgaagc acgatgccga ccacccgcag acggaaccct tcgtcgtggt gggctacgac
    31921 gcgcggcaca agtccgacga gttcgcgcgg gacacggccg cggtggtgac cgctgcgggt
    31981 gggcgagccg tcgtgctgcc gcacgtgctg ccaactccgg tgctggcgtt cgcgattcgc
    32041 tacctcggcg ccgacgcggg ggtcatggtg accgcgagtc acaacccgcg ggaggacaac
    32101 ggatacaagg tctatctcgg cgacggctcg cagatcgtgc cgcctgccga cgccctcatc
    32161 gcggcacgga tcgcggccgt agggtcggtg gcggatgtgc cgctcgcgac tgacgggtgg
    32221 gagacgttag ggccggacgt gctcgacgcg tatctcg
//
LOCUS       DAOMZY010000006        31037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_1130_length_31037_cov_6.944968, whole genome shotgun sequence.
ACCESSION   DAOMZY010000006 DAOMZY010000000
VERSION     DAOMZY010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 31037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31037
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1130_length_31037_cov_6.944968"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PLK64_00765"
     CDS             <1..502
                     /locus_tag="PLK64_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HPV78301.1"
                     /translation="AAVPGVQFGAERASGAMVEPGYPGADLDRGAEAVRVAPIQDRGG
                     EPVGKLLAVAPHWDEADFLDRVASVVGDRAHLAYSGAHGLAEITGPGVTKAAGLARWA
                     GDLGVGSTDVWAFGDMPNDLPMLRWAGRSYAVANAHPDVLAAASHRCPANSQDGVAWA
                     LESLVR"
     gene            671..2320
                     /locus_tag="PLK64_00770"
     CDS             671..2320
                     /locus_tag="PLK64_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPV78302.1"
                     /translation="MADLRLDDDERKLAELGYKQELSRSWSGFSNFAISFSIISILAG
                     CFTTFGQAWNNGGPVAISIGWPLISIFILVIGLCMSELVSAYPTSGGIYWWAAKLGGA
                     KAGYYTGWLNLIGLIAVDASVAYGCATFFDLTFSTFSESWAEGYSLARVFWMFLVILI
                     LVAVVNIFSGHLMAVMNNVSVWWHVFGALAVILILVFLPKSHMSVADVFTMRVNNSGG
                     LAGGSTDGLGYWFYVLPLGFLLTQYTITGFDASAHLSEETQGASHGAARGIWQSIFYS
                     AIGGWILLLAFLFAVQDAEEVTAGGGGVAVIFNQALTSGWAGIVLLIASIGQFFCTTA
                     CMTSTSRMLFAFSRDGAVPGASLWAKLNSSKVPANAVLASTVVAALITIPALKEVNIG
                     TEDAPIIVPTAFYAVVSVAVIGLYLAFLIPIYLRWRAGDKFKQGPWNLGTKWKWMAPI
                     ACLEIVVISVYFILPFTPAAWLGNEAFDWKFVNYAPILTGGTLILLWIGWHASAKKWF
                     TGPKTTIDLPAGVSSAEEIAMEHHGKSAHPAQDADGKRPSS"
     gene            2386..3249
                     /locus_tag="PLK64_00775"
     CDS             2386..3249
                     /locus_tag="PLK64_00775"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HPV78303.1"
                     /translation="MTELPGGLTNHNYRVRTATADVVVRVSPPGTDLLAVDREVEHHN
                     SAAAAAAGVGAPVIDYLPGRGVLVVGFLTARTYSEDDVAANLPRIAAALRRLHAGPPF
                     VGRLDMFALQRRYLALAHERGMCMPSGYDSLAPVVARAEAALAAYPEPLVPCHNDLLA
                     ANFLDDGQDVRIIDYEYSGLNEPAFELGNIAQESGLSDAALVGLVEAYYPHADARLLA
                     RARTWRAISGYGWTLWGVIQHHVSGIDADFWGWATHKYDTAATLLRDSAFVDTLSVAA
                     AAPAPTPRAPA"
     gene            3246..5756
                     /locus_tag="PLK64_00780"
     CDS             3246..5756
                     /locus_tag="PLK64_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPV78304.1"
                     /translation="MSPTDTVPSRARVVIIGGGVIGASVAYHLTALGWTDVVLLEQGT
                     LSCGTTWHAAGLVGQLRASEAGTRLVQYSCELYDRIEAETGLSAGYRRCGGLTVARTP
                     ERMVTLRRTAASAAAYGLDCDLLTPDQAGERYPLIRTDDLVGAIWLPADGRANPTDLT
                     QALAKGAKQRGATIFERTRVLDIHTGPATRGVRVTGVSTDRGDIEAEIVVNCAGQWAK
                     AVGELVGATVPLHSAEHFYVVTEQIEGVHRDLPILRDPDGYTYVKEEVGGLLVGGFEP
                     VAKPWVAPDQIPYPFEFQLLDEDWEHFEILMSSAVHRLPVLAETGVRKFYNGPESFTP
                     DNQFLLGEVPGVAGFFVGAGFNSVGIASAGGAGRALAEWIVEGEPTSDLTAVDIRRFA
                     PFNANNDWLRDRVGEVLGLHYSIPWPNRELETARPFRRSPVHDRLVGAGAVLGSKMGW
                     ERANVFAPRGVEPVLDYSWEAPTWLPWSRAEQAATRHTVSIFDQTSFAKYLVTGPDSA
                     ATLQWLCTADVDVPIGRAVYTGMLNSAGGYEADVTVTRLAPTQFLVVSGAASAVRDVD
                     WIRRKAPDGHRTEVVDVTAMYAVFGVMGPASRELLTRLSGADLTDAAFPFATSRELRL
                     GYATARATRITYVGELGWELYVPVEFAAGVYDELFNAGHLLGAAPAGYYTIDAMRLEK
                     GYRAFGRELVPDATPIEAGLSFTCKLASGIDFLGRSAVERVKTAGPSRRIVSIVVGDP
                     TAEPLAGDPAAYLWGGELLLRDGEPVGQVTSAGWGHALGAPVGLAWAGSRQTGPVTPE
                     WLRTGGYEVDVAGRRVPVTVSLRPPFDPDGVRLGRGPA"
     gene            5753..6490
                     /locus_tag="PLK64_00785"
     CDS             5753..6490
                     /locus_tag="PLK64_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HPV78305.1"
                     /translation="MIIPLHNDLSEHVLRPSSGNAFELTVEQLATAIRLGAFRDGDLL
                     PPERELAERLAVSRSTLREAIAALRDSGLVTTRRGRGGGTMVTYAGEAPGSVPLPQPQ
                     RTATAIADVLDFRRVVEPGAAALAATRTLAADQRAWLLACAAQVREAPDSATHRLNDS
                     RLHLALATLSSSPMLVEAVTRAQAALHELLRGIPVISRNIAHSHDQHDAIVAAVLAGD
                     PETARAVMEEHCDATSLLLRALLRPRP"
     gene            6519..7883
                     /locus_tag="PLK64_00790"
     CDS             6519..7883
                     /locus_tag="PLK64_00790"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009379784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HPV78306.1"
                     /translation="MTRPELTLDQLRAEIADGTIDTVVVAFTDMQGRLQGKRIHGQWF
                     VDTVLGHGTEGCNYLLAVDVEMNTVDGYAISSWETGYGDMMFDLDLTTLRRTPGQNYS
                     ATVQCDLAWLDGRGLVRQSPRSVLRAQVDAAAALGFTALAGTELEFIVFEQSYAAAWD
                     ARYVGLTGVNRYNVDYSIQGGTVVEPLLREIRNEMYAAGLKVESAKGECNLGQHEIAF
                     RYEDVLRTCDNHVIYKNAAKEIAAKHGQSLTFMAKYDEREGNSCHIHLSLRGTDGSTV
                     FADDGREGGHSDVFDHFLAGVQATLRELTYFYAPNINSYKRFQPGSFAPTAIAWGRDN
                     RTCALRVVGHGSGLRVENRVPGGDVNPYLAVAAMLAGGLHGIREGLALEPRFDGNAYA
                     SDKARVPTTLVEARELLLGSAVARAAFGDEVVEHYAHAAQVEVDAFNRAVTDWELIRG
                     FERL"
     gene            7915..9291
                     /locus_tag="PLK64_00795"
     CDS             7915..9291
                     /locus_tag="PLK64_00795"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPV78307.1"
                     /translation="MSITHEVINPSTEQVVATVPSLSVEETDAAIARAEAAFPAWRAV
                     SPADRGRLLRRFSEVVDAHIDELAQLEVTGSGHTIGNARWEAGNVRDVLAYYAAAPER
                     LFGKQIPVAGGLDVTFKEPFGVVSVIVPWNFPMPIAGWGFAPALAAGNVVVLKPAELT
                     PLTAIRLGELALEAGLPEGVFTVLPGKGSIVGERFVTHPAVRKVCFTGSTEVGQRIMR
                     GAAEQIKRVTLELGGKSANIVFADSDLEKAAASAPMSVFDNSGQDCCARSRILVQASV
                     YDRFMELFETAVKGVVVTDPADEAAQMGPLISADHRAHVRGFVEGSADSVDVAFRGTA
                     PTGAGWWYPPTVVIPRSTQDTVWQEEIFGPVVAVLPFDDEADAIAKANDTAYGLSGSI
                     WTRDVGRAIRVARGVEAGNLSVNSHSSVRYATPFGGFKQSGLGRELGPDALDAFTEVK
                     NVFFATDD"
     gene            9344..10111
                     /locus_tag="PLK64_00800"
     CDS             9344..10111
                     /locus_tag="PLK64_00800"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HPV78308.1"
                     /translation="MSGRLDGKVAVITGGCSGIGLATVRRFVAEGAKVVVGDLNDEAG
                     AALADELGILYVRCDVTSKEAVDAMFAAAKEHFGSVDIAFNNAGISPPDDDSILDTDL
                     DAWRRVQEVNLTSVYLCCKAALPYMIEQGKGSIINTASFVAVMGAATSQISYSASKGG
                     VLTMTRELGVQFARQGIRVNALCPGPVNTPLLQELFAKDAERAARRLVHIPMGRFGEP
                     EEMASAVLFLASDDSSFMTASTFLVDGGISAAYVTPL"
     gene            10136..10912
                     /locus_tag="PLK64_00805"
     CDS             10136..10912
                     /locus_tag="PLK64_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HPV78309.1"
                     /translation="MGQPRCPVIGITCYVESIDRPPWVSQPSAVLPRMYLEHVARAGG
                     IPVILPPGDEHLTDAEADAQAREVLDRVDGLIIAGGADVQASRYAALPHPTSQAPRPD
                     RDAWELALARVSAETGIPILGVCRGMQIMAVANGGTLDQHLPDTVGTDVHLQTAGVFT
                     RHTVRVADGSHLAGVLGAETVEVPTYHHQGVATHPGYAATAWTADGVVEAFEDPQARF
                     RLAVQWHPEAAEDSSLFRSLVAAATTWSASRDTLSGSGPE"
     gene            11103..12692
                     /gene="cimA"
                     /locus_tag="PLK64_00810"
     CDS             11103..12692
                     /gene="cimA"
                     /locus_tag="PLK64_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HPV78310.1"
                     /translation="MSTASPELHVYDTTLRDGAQQEGLNLSVVDKLAIARQLDELGVG
                     YIEGGWPGANPKDTEFFARAQQELDLRNATLAAFGATRRAGSTAAADPLVRALLDSGA
                     SVVTLVAKSHARHVELALRTTLAENLEMIRDTVTFLRGEGRRVFLDAEHFFDGYRLDR
                     AYALECVRTAAEAGAEVAALCDTNGGMLPAQIADVVRDVLDSTGARVGIHCHNDTGCA
                     VANSMAAVAAGATHVQGTVNGYGERTGNANILTLVSNLQIKQGMPLLEPQRLREATRI
                     SHAISEVTNVPPYSRQPYVGSSAFAHKAGLHASALKVDPDLYQHTDPALVGNAMRTLV
                     SDMAGRASIELKAKEMGVDLTDQSELVAAVLAEVKELELRGWTFDAADASFELLLRKH
                     LGQAVTYFDVESWRVITDAREAGEAVSEATVKLTAGGSRQVATGEGNGPVNALDHALR
                     VALMGVYPELARLELVDFRVRILDASHGTDAITRVLIETSDGTTTWQTVGVAPNIVEA
                     SWRALVDGITYGLSRQGVSAR"
     gene            12689..13264
                     /locus_tag="PLK64_00815"
     CDS             12689..13264
                     /locus_tag="PLK64_00815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV78311.1"
                     /translation="MSIDHAAPPQTAHATRHTAPAAGVGLVLRPARADEAPLLSELAL
                     RSKGWWGHSASFLRACEAELTLSPEQAAKAVLVEVDGVVAGFHLLAPSSVGSDVGELD
                     MLFVDPPFIGSGIGRILFEDARRSAAGRGWTRVQIEADPQARPFYERLGAVQVGERLS
                     RSVPGRSLPLFELTLRGGGSSRPIGRATYWR"
     gene            complement(13341..14261)
                     /locus_tag="PLK64_00820"
     CDS             complement(13341..14261)
                     /locus_tag="PLK64_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPV78312.1"
                     /translation="MLMGIDIGGTKTAVVALGPDGSVVAERRAPSGHGPDGVVAVALA
                     VAHECADAVGGWTQIASVGACMPGLVDPRSGLVTHALNLGVDALPLGPALSDALSRPV
                     VIDNDVKAAGWGAFQLHGGDPDTAFGYLNIGTGLAAALVINGQIVRGPGGIAGEIGHL
                     PLAAPVPCRCGQTGCLETSASGSGLTRMWPPARHLADPFAAAAAGDAQAAEAVRHLCG
                     GIAMAIQALVLVGGVEHIVIGGGLTGLGEPLSLGIAEVLAQRAAQAPLLDELTQPGRF
                     SMLSSDVPIAALGAARLPSAAAQLDLQVAP"
     gene            complement(14255..15724)
                     /locus_tag="PLK64_00825"
     CDS             complement(14255..15724)
                     /locus_tag="PLK64_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPV78313.1"
                     /translation="MAPTQLRALAASTLMPGFVGTTVPAWIDEAVDGGLRTVCLYGSN
                     ITDRAQTHRLCRSLRGISTSLLIAVDEEGGDVTRLHFPSGSPEPGNAVLGRLDDVAST
                     RRSAARIGADLAAYGIGLDLAPVVDVNSADDNPVIGTRSFGADPEVVARHTVAYVEGL
                     ATQSVAACAKHFPGHGDTHTDSHLDQPVVLADLDTLHRRELVPFRAAIEAGVATVMTS
                     HILVPALDPSAPATFSEPILQGVLREELGFTGVVVTDALDMAGASATQGIPTAAVRAL
                     AAGADLLCLGSDTTPEGYAAVLDAIVAAVDAGVLDPRRLKDAAVRVAALADVHSLAVG
                     DSQPAAGALPSPTARADVASATIAGTFSVSPSLAAWRAGTGPVAIVQVDTRANLAVGP
                     TNWGPAALGATIDPGQVPTDARVAVVGRGIGVDHPARAAAAAYGASGHPVVLVECGWP
                     FGDVDVDVDVATYSGSLSVAAALVELLTTSPREALVQPC"
     gene            complement(15726..16565)
                     /locus_tag="PLK64_00830"
     CDS             complement(15726..16565)
                     /locus_tag="PLK64_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPV78314.1"
                     /translation="MTLRRIGRALLTVLTLLLALVWAFPVYWMVNSSMLSNVTLQSTT
                     PTFLPFGGSFDNFAAVFSDPGFLQALGVSLAVTLGTVAASLFIAFLGALAISRFRFRG
                     RKSFILALLLIQMLPAEGLFIAQYKMMSGIGMLNSVLGLTILYTAAVVPFTVWMLRGF
                     VAGVPVDLEEAGMVDGLTRTQAFLRITFPLLAPGLVASGVFAFLQAWNEFTIALVIMT
                     DEQSRTLPLWLRGFVQASASRETDWGQVMAASTVVAVPVIIFFLLVQSRMSSGLVGGA
                     VKG"
     gene            complement(16565..17455)
                     /locus_tag="PLK64_00835"
     CDS             complement(16565..17455)
                     /locus_tag="PLK64_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPV78315.1"
                     /translation="MPVRRRSRLPHILITPTIIVLIAALGYPVAWQIITSFKEFGLRQ
                     QFGVPADWVGLANYVEIVSDPALWAIVLRSLVFCLVVSFATVLIGGALAGLMNSVHKA
                     ARLTLQVALLLAWAMPAVASMTVWIWLVDWRRGLLNWALVRLGVDAQGHNWLADPTSF
                     LIIAAIIVTWMSVPFVTLSIYAGLTQVSPEVLEAAKIDGASGIQRLRHIIVPLIAPVL
                     SIVLLLQFIWNLRVFTQVKILQDAGGVANETHLLGTYIYQLGTGKGDFGSASAVSILM
                     LLLTIAISWRYVRNLMKEES"
     gene            complement(17584..18942)
                     /locus_tag="PLK64_00840"
     CDS             complement(17584..18942)
                     /locus_tag="PLK64_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPV78316.1"
                     /translation="MKKNLQAALVVGTAATLLLTACSGGSTGSAPTATNTAANASKSI
                     TLWLAGGDTPAELRTYLADTFKAKTGATLKIQEQSWGDLTTKLTTALPDANNTPDVVE
                     LGNTQSPTFTNVGAFLDISDMYNELGGAKLLPSFVEAGSVDGKQFALPYYFGSRYLFQ
                     RKDVWTQAGVATPKTLDEFNTAVKTITTANPRKIANFSGFFIGGQDWRNGISWVFANG
                     GDLAKKESGQWVSTLSDPNTIKGLTQLQDIYRNASKAPADAKDASPWLYINDTDQILD
                     DTKAVTGTTSLAAATIMAPGWAHWSIGDLATKEGKPTRQWNDATFGVFVLPGNDGKPA
                     PVFAGGSNIAISNTSKNKDLAKELMRIIFSADFQKKLGGAGLGPANADYVSSLGTDAF
                     ATATIQSASNSKLTPAAPGWAAVESAKVMEEFFGKIRDAADLTALAKEYDAKITPMLN
                     LK"
     gene            complement(19072..20286)
                     /locus_tag="PLK64_00845"
     CDS             complement(19072..20286)
                     /locus_tag="PLK64_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HPV78317.1"
                     /translation="MSSVRPQRVRPSAPVQPSHARRQNLSLALQALYSSGPMSRADLA
                     RSLGLTKVTVSELVTALLDRGHAIEVGRSTPGRMGKPSILVDVNRTGLQMIGLDLSDH
                     RALHGAVLDLDGTVVARAQRPVLADDSVIGDGSTAGPDRPAGPLGGERAVDLVLELVA
                     ECLALATAPILGIGIGTPGIVGSDGVIKTAPNLDWRDVPLRDIVASATNLPVSLCNDA
                     DAAAHADHTLGDGGDDLVLVKIGRGVGCGLIVGGRRVRGAHSAAGEIGHVVVGTDGGR
                     RCRCGKVGCLETWVSAPRLEQAIAASTPDPADAAPHADDVPDAVTAAGERLAIALAPV
                     VAALDLADVVLCGPAHVIVPALVESVERTLRARLFTVENATLRVRVVEDPNDIVVRGA
                     AALVLLDQLGVS"
     gene            complement(20361..21245)
                     /locus_tag="PLK64_00850"
     CDS             complement(20361..21245)
                     /locus_tag="PLK64_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyladenine DNA glycosylase"
                     /protein_id="HPV78318.1"
                     /translation="MTQVLAEQEWRAAAAAHADRVDALTAGHRERRARGIPHPVEDFV
                     FTYYPFTPSAIRRWHPGLGVRLAGAAADPRAQWRHYRIVDDGVELDVAAYLAARGDTL
                     TFVRDLLTRTADRAPQLGCFGLHEWAMVYRQRPDEVRHSRWPLRLGRAGTDAVVEANP
                     LRCSHFDAFRFFTEPARPLNLLTPTRASQADLDQPGCLHATMDLYKWAMKLAPAIPSE
                     LVADTLELALDLRVLDMRASPYDLRDLGIEPIAVETPEGKATYAGEQRRYAARGQALR
                     MRLLSACASALAERASID"
     gene            21299..22078
                     /locus_tag="PLK64_00855"
     CDS             21299..22078
                     /locus_tag="PLK64_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPV78319.1"
                     /translation="MRIARYTTGEDPAYGIVDGERGAEVIHPLKGDPLYVGIVPAGLE
                     PIALEEVRLLAPVIPRSKVIGIGRNYADHAKEFGNEVPAEPMMFLVPNTAVIGPGDPV
                     VIPRGSATVDYEGELALVIGKICKDVRPEQAMECVWGYTCANDVSERTWQRGDGQWSR
                     AKGFDTSKPLGPWIVTDLDPSALRLVTRRDGEVVQDGTTADLVHDVATLVSYASTYFT
                     LLPGDVILTGTPAGVGPVQAGQRVSVEIEGIGTLDNPFVRR"
     gene            complement(22110..23060)
                     /locus_tag="PLK64_00860"
     CDS             complement(22110..23060)
                     /locus_tag="PLK64_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HPV78320.1"
                     /translation="MSGGLAALLDDIAAIAKLAAASIDDVGAAAGRASVKAAGVVVDD
                     TAVTPRYVQGFTPDRELPIIKRITIGSIRNKLVFILPAIMLLSQFLPWALTPLLMVGG
                     TYLCFEGAEKIWEKVSGHDAGEEAPAIVQGEDHEKTMVAGAIRTDFILSAEIMVIALN
                     EVATEPFLSRLIILIVVALGITVGVYGFVALIVKMDDIGLHLSERQSGAARAIGRGLV
                     AAMPVLLRWLAIIGTVAMLWVGGHILLVGADTLGWHGPYALVHHAEEAVAAAVSVGSG
                     ALAWLTNTLGSAIAGLIVGAVVVLIMHLIPRKKDAANAAH"
     gene            23154..24677
                     /gene="gltX"
                     /locus_tag="PLK64_00865"
     CDS             23154..24677
                     /gene="gltX"
                     /locus_tag="PLK64_00865"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPV78321.1"
                     /translation="MSEKQLPGAVRLRVAPSPTGDPHVGTAYMSMFDLAFARQQDGRF
                     LLRIEDTDRARLQEDSERQIYDTLHWLGLTWDEGPDIGGPCAPYRQSERLETYRPFVE
                     RLLEAGHAYLCWCSAERLTAMREDQQRRKVPTGYDRLCLGKTRKERAALPGFSETPVV
                     RMQIPDDVQTSFDDLIAGRIDAPVPDDQVILKADGFPTYHLAVVVDDHLMGITHVVRG
                     QEWISSTPKHLLLYRWLGIDPPAFAHMPLLRDERKAKISKRKSPWARLTWFREQGYLP
                     EALVNFLALQGYPPIVEADGTEREVFTFDEFAARFEWSKVNPAGSIFNLDKLDWLNGV
                     WIRELPVGEFASRLLPFLEADGILSDNPSLGELARLRSIAALIQTRMVKLTEASALVA
                     PFFMGDDVIAVADDARAQLDAQSPAVLDAAMHALGDIDDHQRGVLGSESDWSAARIEA
                     ALRSAIVEGLGVKPKVAFGPLRTAVSGQRISPPLFESMEILGKTSTLARLQALRDTV"
     gene            24769..24840
                     /locus_tag="PLK64_00870"
     tRNA            24769..24840
                     /locus_tag="PLK64_00870"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24802..24804,aa:Gln,seq:ctg)
     gene            24942..25014
                     /locus_tag="PLK64_00875"
     tRNA            24942..25014
                     /locus_tag="PLK64_00875"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24976..24978,aa:Glu,seq:ctc)
     gene            complement(25105..26106)
                     /gene="fgd"
                     /locus_tag="PLK64_00880"
     CDS             complement(25105..26106)
                     /gene="fgd"
                     /locus_tag="PLK64_00880"
                     /EC_number="1.1.98.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase
                     (coenzyme-F420)"
                     /protein_id="HPV78322.1"
                     /translation="MRIGYKASAEQFGPRELVELAVLAEQVGLDSVWVSDHVQPWRHV
                     GGHAPFSLAWLAAAGERTSRVQLGTSVLTPTFRYNPAVLAQAFATLGVLNPGRIALGV
                     GTGEALNEFATGAVAEWPEFKERFARLREAIALIRTLWTEDRVTFEGDYYRTTDATIY
                     DRPEVPVPVYIAAGGPLVARYAGRAGDGFICTSGKGEELYRDQLLPAVDEGLTKAGRD
                     GVGYDRMIEVKLSWDPDRAAAVANCRFWAPLSLTAEQKHGTTDPIEMERLADELSDEQ
                     VARRWIISNDPDEVVEAVRPYVGWGFDHLVFHAPGHDQHRFLTTFAESVLPRLRELG"
     gene            complement(26200..27297)
                     /locus_tag="PLK64_00885"
     CDS             complement(26200..27297)
                     /locus_tag="PLK64_00885"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HPV78323.1"
                     /translation="MTRRIGRAARVGIISLAGVLVASACTAAPTPAAAPATAVVTPVA
                     AQTSVTPTPSATPTATATPTAVPPLLAEGADGDQVRELQARLKQLKNFTSTVDGVYDA
                     ETTGAVQAFQTALKLPASGSVDETTWSTLLATTKAPSAEDLAGHLIAGPAILASGSSG
                     AKVRELQARLKQLGRWTGDVTDSYGTKTVSAVKGYQTKRGLPVTGEVDQRTMDRIWGQ
                     TRKPTSNELNNVKPAPGAGLTTAGLDPRCLVGRVICASKSTRKVVWVVDGVPVMQMDA
                     RFGRAELPTNDGAFHIYWKSIDHYSKKYDAPMPYALFFNGGEAVHYSPEFASIGYSGV
                     GSHGCVNTRDKAKMAWLFNEARVGDLVVVYR"
     gene            complement(27427..28158)
                     /locus_tag="PLK64_00890"
     CDS             complement(27427..28158)
                     /locus_tag="PLK64_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPV78324.1"
                     /translation="MDNSSGVGVLDKAATVLSALEAGPATLAQLVGATGLARPTAHRL
                     AVALEHHRLVTRDMQGRFVLGPRLAELASAAGEDRLLAAAGPVLGALRDHTNESAQLF
                     RRQGDLRICVAAAERPMGLRDTIPVGASLSMLAGSGAQILLAWEEPDRLHRGLQGARF
                     NATALSAVRRRGWAQSVGEREPGVASVSAPVRGPSGRVVAAVSISGPIERLSRQPGRL
                     HAATVVAGANKLTEVLRRAHQSTPS"
     gene            28265..29665
                     /gene="leuC"
                     /locus_tag="PLK64_00895"
     CDS             28265..29665
                     /gene="leuC"
                     /locus_tag="PLK64_00895"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HPV78325.1"
                     /translation="MAGTLAEKVWEAHVVRRAEGEPDLLYIDLHLLHEVTSPQAFDGL
                     RLEGRTVRRTDLTLATEDHNVPTTPGPITDLISLTQVETLRRNCVEFGVPLYPMGDVE
                     QGIVHVVGPQLGLTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLPLKP
                     FRTMAVNVRGALPDGVTAKDVVLAVIAKIGTGGGQGYVLEYRGDAIESLSMEARMTVC
                     NMSIEAGARAGMIAPDDTTFDYLKGRDRAPVGADWDAAVEAWRQLRSDDDAVFDAEVD
                     LDATTLSPFVTWGTNPGQGLPLSASVPDPDRIVDETERADTERALAYMGLEPGTPLRE
                     IPVDTVFVGSCTNGRIEDLRAAADVLRGRRVAEGVRMLVVPGSARVRVQAEAEGLHSV
                     FEAAGAEWRLPGCSMCLAMNPDKLAAGERSASTSNRNFEGRQGPGGRTHLVSPLVAAA
                     TAVRGTLSSPADLDKD"
     gene            29670..30257
                     /gene="leuD"
                     /locus_tag="PLK64_00900"
     CDS             29670..30257
                     /gene="leuD"
                     /locus_tag="PLK64_00900"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HPV78326.1"
                     /translation="MDAFTTHTGIGVPLRRSNVDTDQIIPAVYLKRVTRDGFEDGLFA
                     AWRTDESFVLNNPRYAAGSVLVAGPDFGTGSSREHAVWALMNYGFRAVLSSRFADIFR
                     GNSGKQGLLTAQVAQDDIELIWKYLEQEPGAQVTVDLAARTVTAGDIVAPFSVDDYTR
                     WRLMEGLDDIGLTLRHDDLIAQFEADRPAYLPVTS"
     gene            30663..>31037
                     /locus_tag="PLK64_00905"
     CDS             30663..>31037
                     /locus_tag="PLK64_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPV78327.1"
                     /translation="MNKAELVVALEDKLGGKKAASDALDAVLDAIIREVAKGGKVAIT
                     GFGTFQKVARAARTGRNPRTGDAVRIKKTTVPRFRPGTSFKGYVADPRSLPKAAPATG
                     RAPAGSVAKAAPAAAPVKKAAPA"
BASE COUNT         4827 a        10563 c        10770 g         4877 t
ORIGIN      
        1 cgcggccgtt cccggggtgc agttcggggc cgagcgggcc agcggggcga tggtcgagcc
       61 gggatatccg ggagccgacc tcgaccgtgg ggcggaagcg gtccgggtcg ccccgattca
      121 ggatcgcggc ggcgagccgg tgggcaaact gcttgcggtg gcgccgcact gggacgaagc
      181 cgacttcctc gaccgggtcg cgtcggtcgt gggcgatcgg gcgcacctcg cctactcggg
      241 cgcccacggg ttggccgaaa tcaccggccc cggtgtgacc aaggcggccg ggctggcccg
      301 ctgggcgggc gacctcggcg tcgggtcgac ggacgtctgg gccttcggcg acatgcccaa
      361 cgacctgccg atgctgcggt gggccggtcg ctcgtatgcc gtggccaacg ctcatccgga
      421 cgtgctcgct gcggcgagcc accggtgccc ggcgaactcg caggacgggg tcgcctgggc
      481 gctggaatcg ctcgtcaggt gacgtccagc tgaacagtgg gctgacaatg gtcgctgacc
      541 atgcctttta ggtccgttct gcgcagatct tgagagcgct ttccctcgca ctcgtgccgc
      601 taaaggtctg gacgcacacc ttcttcgcta ctagcatcgc ctcacttcga ccgtgtgagg
      661 agcgtcacat atggctgacc ttcgattgga cgacgacgag cgcaagctcg ccgagctggg
      721 ctacaagcag gagttgtccc gatcctggtc gggcttctcg aacttcgcga tctctttctc
      781 gatcatctcg atcctcgccg ggtgcttcac gaccttcggc caggcctgga acaacggcgg
      841 ccccgtcgcg atctcgatcg gctggccgct catctcgatc ttcatcctcg tcatcggctt
      901 gtgcatgagc gagttggtgt cggcctatcc cacctccggg ggcatctact ggtgggcggc
      961 caagctcggc ggggccaagg cgggttacta caccggctgg ctcaacctca tcggcctcat
     1021 cgcggtcgat gcctcggtcg cctacgggtg tgcgaccttc ttcgacctca ccttcagcac
     1081 cttcagtgag tcctgggccg agggctactc gctggcccgg gtgttctgga tgttcctcgt
     1141 catcctcatc ctcgtggccg tggtcaacat cttcagcggc cacctcatgg cggtgatgaa
     1201 caacgtctcg gtctggtggc acgtcttcgg ggccctggcc gtcatcctca tcctcgtctt
     1261 cctgcccaag tcccacatga gcgttgccga cgtgttcacg atgcgggtga acaacagcgg
     1321 tggtctggcc ggcggttcga ccgacgggtt gggttactgg ttctacgtgc tgccgctggg
     1381 cttcctgctc acgcagtaca cgatcaccgg cttcgacgcc tcggcgcacc tgtccgagga
     1441 gacccagggg gcctcgcacg gtgcggcacg cggcatctgg cagtcgatct tctactcggc
     1501 gatcggaggc tggatcctcc tgctcgcctt cctcttcgcc gtccaggacg ccgaggaggt
     1561 gaccgccgga ggtggcggcg tggccgtcat cttcaaccag gccctcacct cgggatgggc
     1621 cggcatcgtc ctgctcatcg ccagcatcgg gcagttcttc tgcacgaccg cctgcatgac
     1681 ctccaccagt cggatgctct tcgcgttcag tcgcgacggc gcggttccgg gagcgtcgct
     1741 ctgggccaaa ctcaactcga gcaaggtccc ggccaacgcc gttctcgcct ccaccgtggt
     1801 cgccgcgctc atcacgatcc cggcgctcaa ggaggtcaac atcgggaccg aggacgctcc
     1861 gatcatcgtt ccgaccgcgt tctacgccgt cgtgtcggtc gcggtgatcg gcctctacct
     1921 cgcgttcctc atcccgatct acctgcggtg gcgcgccgga gacaagttca agcagggccc
     1981 gtggaacctc gggacgaagt ggaagtggat ggccccgatc gcctgcctgg agatcgtcgt
     2041 catctcggtc tacttcatcc tccccttcac cccggccgcc tggctgggca acgaggcctt
     2101 cgactggaag ttcgtcaact acgcgccgat cctcaccggc gggacgctca tcctgctctg
     2161 gatcgggtgg cacgcgtcgg cgaagaagtg gttcaccggt cccaagacga cgatcgacct
     2221 gcccgcggga gtctcctcag ccgaggagat cgccatggag catcacggca agtcggccca
     2281 cccggcgcag gacgccgacg gcaagaggcc gtcgtcctga tcgtcgactc gactctcgcg
     2341 cggctgctcg acgagatccc ctgtctctca gggcagccgc gcgaggtgac cgaactcccg
     2401 ggtggtctca ccaaccacaa ctaccgggtt cgcacggcca ccgcggacgt cgtcgtccgg
     2461 gtgtccccac ccggcacgga cctgctcgcc gtggaccgcg aggtggaaca ccacaactcg
     2521 gcggccgccg ccgcggcggg ggtcggtgcc cccgtcatcg actacctgcc aggccgcggc
     2581 gtgctcgtcg tggggttcct gaccgctcgc acctactcgg aggacgatgt ggctgccaac
     2641 ctgccgcgca tcgccgcggc gctgcgccgg ttgcacgccg gcccgccgtt cgtcggtcgg
     2701 ctggacatgt tcgcgctgca gcggcgttac ctcgcgctcg ctcacgaacg agggatgtgt
     2761 atgccgtccg gctacgactc gctcgcgccg gtggtcgcgc gtgcggaagc cgcgctcgcg
     2821 gcatacccgg agccgctcgt cccgtgccac aacgatctgc tggccgcgaa cttcctcgat
     2881 gacggccagg acgtgcggat catcgattac gaatacagcg ggctcaatga gccggccttc
     2941 gagttgggca acatcgctca ggagtcgggt ctgagcgacg ccgccctcgt cggcctcgtc
     3001 gaggcgtact acccgcacgc cgacgcgagg ctgctcgcgc gggcgcgcac ctggcgggcg
     3061 atctcgggtt acggctggac cctctggggg gtcatccagc accacgtcag tgggatcgat
     3121 gccgacttct ggggctgggc cacgcacaag tacgacacgg cggcgacgct gctgcgggac
     3181 tcggccttcg tcgacaccct ctcggtcgcc gcagccgcac cggccccgac acctcgggca
     3241 ccggcatgag ccccaccgac accgtgccca gccgcgcccg cgtcgtcatc atcggaggcg
     3301 gcgtcatcgg agcgagcgtc gcctaccacc tcaccgccct gggctggacc gacgtggtgc
     3361 tcctggagca gggcaccctc tcgtgcggca ccacctggca cgcggccggg cttgtcggtc
     3421 aactgcgcgc ctcggaggca ggcacccgcc tggtgcagta ctcctgcgag ctctacgacc
     3481 ggatcgaggc cgagaccggg ctgtcggcgg gctaccgtcg ctgcgggggc ctcaccgtgg
     3541 cgcgcacccc cgagcggatg gtcaccctgc gccggacggc ggccagcgca gcggcatacg
     3601 ggctggactg cgacttgctc accccggatc aggcggggga gcgctacccg ctgatccgca
     3661 ccgacgacct cgtcggagcg atctggctgc cggccgacgg gcgagccaac ccgaccgacc
     3721 tcacccaggc cctcgccaag ggcgccaaac agcgtggcgc gacgatcttc gagcgcaccc
     3781 gggtcctgga catccacacc ggtcccgcca cccgcggggt gcgggtcacc ggggtgagca
     3841 ccgaccgcgg cgacatcgag gcggagattg tcgtcaactg cgccggccag tgggccaagg
     3901 ccgtcggcga actcgttggt gccaccgtgc ccctgcactc ggccgagcac ttctacgtcg
     3961 tcaccgagca gatcgaggga gtccaccgcg acctgccgat cctgcgcgac cccgacggct
     4021 atacctacgt caaggaagag gtcggcgggc tgctcgtcgg cgggttcgaa ccggtcgcca
     4081 agccgtgggt cgcgcccgac cagatcccct acccgtttga gttccaactg ctcgacgagg
     4141 actgggaaca cttcgagatc ctcatgagca gcgcggtcca ccggctcccg gtgctggccg
     4201 agaccggggt gcgcaagttc tacaacgggc ccgagagctt cacgccggac aaccagttcc
     4261 tcctcggcga ggtgcctggg gtcgccgggt tcttcgtcgg cgccggtttc aactcggtcg
     4321 gcatcgcctc ggccggggga gcggggcgag cgctggccga gtggatcgtc gagggcgagc
     4381 cgacgagcga cctcactgcc gtcgacatcc gccggttcgc gcccttcaac gccaacaacg
     4441 actggctgcg cgaccgcgtc ggcgaggtcc tcgggctgca ctactcgatc ccgtggccca
     4501 accgtgagtt ggagaccgcg cggccgttcc ggcgctcacc ggtccacgac cggctcgtag
     4561 gcgcgggtgc cgtgctcggt agcaagatgg gctgggaacg ggccaacgtc ttcgcccctc
     4621 ggggggtcga gccggtcctg gactactcct gggaagcccc cacgtggctg ccctggtcgc
     4681 gggccgagca ggcggcgacg cggcataccg tcagcatctt cgaccagacc tcgttcgcga
     4741 agtaccttgt caccggcccc gactcggcgg cgacgttgca gtggctgtgc accgcggacg
     4801 tcgacgtgcc gatcggtcgc gcggtctaca cggggatgct caactccgcc ggcggctacg
     4861 aagccgacgt cacggtcacc cgactcgccc cgacgcagtt cctcgtcgtc agcggcgccg
     4921 cctcggccgt gcgcgacgtc gactggatcc gccgcaaggc gcccgacggc caccgcaccg
     4981 aggtcgtcga cgtcaccgcg atgtatgccg tgttcggcgt gatggggccc gcctcacggg
     5041 agttgctcac gcggctctcc ggcgccgacc tcaccgatgc ggccttcccg ttcgccacca
     5101 gccgcgaact gcgacttggc tatgcgaccg cccgcgctac ccggatcacc tatgtcggcg
     5161 agttggggtg ggagctctat gtccccgtcg agttcgctgc cggcgtctac gacgaattgt
     5221 tcaacgcggg ccatttactc ggtgccgctc ccgcgggcta ctacacgatt gacgcaatgc
     5281 gcctggagaa gggctaccgc gcgttcggcc gcgagctcgt gccggatgcc accccgatcg
     5341 aggctgggct gagcttcacc tgcaagctcg cctccgggat cgacttcctc ggtcgatcgg
     5401 cggtcgagcg ggtcaagacc gccgggccgt cgcgtcggat cgtctcgatc gtcgttggtg
     5461 accccactgc cgaacccctg gcaggcgacc ccgcggcata cctctggggt ggggagttgc
     5521 tcctgcgcga cggcgagccg gtcggccagg tgaccagcgc cggatggggg catgccctgg
     5581 gagcccccgt ggggctggcc tgggcgggct cgcggcagac tgggccggtg actcccgaat
     5641 ggttgcgcac cggaggatac gaggtggacg tcgccggtcg acgcgtgccg gtgaccgtct
     5701 cgctgcgtcc ccccttcgac cccgacgggg tccggctggg cagggggccg gcgtgatcat
     5761 ccccctgcac aacgacctgt ccgagcatgt cctgcggccc agttccggca acgccttcga
     5821 gctcaccgtc gagcagttgg cgaccgcgat ccgcctcggc gcgttccgcg acggcgacct
     5881 gctcccaccc gaacgcgagc tcgccgaacg ccttgctgtg tcgcgcagca ccctgcgcga
     5941 ggcgatcgcc gccctgcgcg actcgggctt ggtgaccacg cgccgcggcc gcggtggcgg
     6001 caccatggtg acgtatgccg gtgaggcgcc ggggtcggtc cccttgcccc agccgcagcg
     6061 caccgcaacc gcgatcgccg acgtcttgga cttccgccgg gtcgtcgagc cgggggcggc
     6121 cgcgctggcc gcgacgcgaa ccctcgccgc cgatcagcgg gcctggctgc tggcctgcgc
     6181 cgcccaggtg cgcgaggccc ccgattcagc cactcaccga ctcaacgact cccggctgca
     6241 cctggccctg gccaccctct cgagttcgcc gatgctcgtc gaggccgtga cccgcgccca
     6301 ggccgccttg catgagttgc tgcgcggcat acccgtcatc tcgcgcaaca tcgcccactc
     6361 ccacgaccag cacgacgcca tcgtcgcggc tgtgctcgct ggcgaccccg agaccgcccg
     6421 cgcggtcatg gaggaacact gcgacgccac gtcgctgctg ctccgtgccc tgttgcgtcc
     6481 ccgcccatga cccgtccccc ctcaccgaag gatcgacgat gacccgaccc gagctcaccc
     6541 tcgaccaatt gcgtgccgag atcgccgacg ggaccatcga caccgtcgtg gtcgccttca
     6601 ccgacatgca aggccgattg cagggcaagc ggatccacgg tcagtggttc gtcgacaccg
     6661 tcctgggcca cggcaccgag ggctgcaact acctgctggc cgtcgacgtc gagatgaaca
     6721 ccgttgatgg ctatgccatc agctcgtggg agaccggcta cggcgacatg atgttcgacc
     6781 tcgacctcac gacgttgcga cggaccccgg ggcagaacta ttcggcgacg gtccagtgcg
     6841 acctcgcctg gctcgacggc agggggctgg tgcgccagtc gccgcgctcg gtgctgcgcg
     6901 cgcaggtgga tgctgccgcg gcgctgggtt tcaccgccct cgccggcacc gagttggagt
     6961 tcatcgtctt cgagcagtcg tatgccgccg cgtgggacgc gcgctacgtc ggcctcacgg
     7021 gggtcaaccg ctataacgtc gactactcca tccagggcgg caccgtcgtc gaaccgctgc
     7081 tgcgcgagat ccgcaacgag atgtatgccg cgggcctcaa ggtcgagtcg gccaaggggg
     7141 agtgcaacct cggccagcat gagatcgcct tccgttacga ggatgtcctg cgcacctgcg
     7201 acaaccacgt catctacaag aacgccgcca aggagatcgc cgcgaagcac gggcaatcgc
     7261 tgaccttcat ggcgaaatac gacgaacgcg agggcaactc ctgccacatc cacctgtcgc
     7321 tgcgcggcac cgacggcagc accgtcttcg ccgacgatgg gcgcgagggt gggcacagcg
     7381 acgtcttcga ccacttcctc gccggtgtcc aggccaccct gcgtgagctg acctacttct
     7441 acgcaccaaa catcaactcc tacaagcgat tccaacccgg cagcttcgcc ccgaccgcga
     7501 tcgcctgggg ccgggacaac cggacctgtg cgctgcgcgt cgtcggtcac ggctctggcc
     7561 tgcgggtgga gaaccgggtg cccggcggcg atgtcaatcc ctacctcgcg gtggcggcga
     7621 tgctcgcggg cgggttgcac ggcatacgcg aggggttggc gttggagccg cggttcgacg
     7681 gcaacgccta cgcctccgac aaggcccgcg tgccgaccac gttggtggag gcccgcgagt
     7741 tgctcctagg ctcggccgtg gcgcgggcgg cgttcgggga cgaggttgtc gagcactacg
     7801 cccacgcggc ccaggtggag gtcgacgcgt tcaaccgggc cgtcaccgac tgggaactca
     7861 tccgcggatt cgaaaggctg tgagcaccac gtgaacacca gactgagcac gacggtgtcg
     7921 atcacgcacg aggtcatcaa cccgtcgacc gaacaagtcg tcgcgaccgt cccgtcgctg
     7981 tcggtcgagg agaccgatgc cgcgatcgcg cgggccgagg ccgccttccc ggcgtggcgg
     8041 gccgtcagcc cggccgatcg tggccgactg ctgcggcggt tctccgaggt ggtcgacgcc
     8101 cacatcgacg aactcgccca actcgaggtc accggctctg ggcacaccat cggcaacgcc
     8161 cgctgggagg ccggcaacgt ccgcgacgtg ctcgcctact acgcggccgc acccgagcgg
     8221 ttgttcggca agcagatccc cgtcgccggc ggtctggacg tcaccttcaa ggagcccttc
     8281 ggcgtcgtct cggtgatcgt cccctggaac ttccccatgc cgatcgccgg ctggggcttc
     8341 gctcctgcgc tcgccgccgg caacgtcgtc gtcctcaagc ccgccgagct caccccgctc
     8401 acggccatcc gcctggggga gttggccctg gaggcggggc tgcccgaagg ggtgttcacc
     8461 gtcctgcccg gcaagggatc gattgtcggc gaacggttcg tcacccaccc ggccgtgcgc
     8521 aaggtctgtt tcaccggctc gaccgaggtc ggccagcgga tcatgcgtgg cgcggccgag
     8581 cagatcaagc gggtcaccct tgagttgggt ggcaagagcg ccaacatcgt cttcgccgac
     8641 tccgacctgg agaaggcagc ggccagcgca ccgatgtcgg tcttcgacaa ctccgggcag
     8701 gactgttgcg cgcgcagccg gatcctcgtg caggccagcg tctatgaccg gttcatggag
     8761 ttgttcgaga ccgcagtcaa gggggtcgtc gtcaccgacc cggccgacga agccgcccag
     8821 atgggtccgc tgatcagcgc cgaccaccgc gcgcacgtcc gcggcttcgt cgagggcagc
     8881 gccgactcgg tcgatgtcgc cttccgcggc accgccccga cgggggccgg ctggtggtac
     8941 ccgccgaccg tcgtcattcc ccgctcgacg caggacacgg tctggcaaga ggagatcttc
     9001 ggcccggtgg ttgccgtgtt gccgttcgac gacgaagccg acgcgattgc caaagcaaac
     9061 gacacggcat acgggctgtc ggggtcgatc tggacccgcg acgtggggcg cgcgatccgc
     9121 gtcgcccgtg gggtcgaggc cggcaacctg tcggtcaaca gccacagcag cgtccgctat
     9181 gccaccccgt tcggcgggtt caagcagtcc gggctcgggc gcgagctcgg cccggacgcc
     9241 ctcgacgcat tcaccgaggt gaagaacgtc ttcttcgcca ccgacgactg accggatcga
     9301 gcgctcgcgc cagtcggttc acgaccccga aggaaggaca cacatgtctg gcaggttgga
     9361 cggcaaggtc gccgtcatca ccggaggctg ctcgggcatc ggcctggcga ccgtgcggcg
     9421 attcgtcgcc gagggtgcca aggtcgtggt cggcgacctc aacgacgagg ccggtgcagc
     9481 gttggccgac gagctcggca tcctctatgt ccgctgcgac gtgacgagca aagaggccgt
     9541 cgacgcgatg ttcgcggctg ccaaggagca cttcggcagc gtcgacatcg ccttcaacaa
     9601 cgccggcatc tcgccgccgg acgacgactc gatcctcgac accgatctcg acgcgtggcg
     9661 ccgggtgcag gaggtcaacc tcacctccgt ctacctgtgc tgcaaggcgg cgctgcccta
     9721 catgatcgag cagggcaagg gctcgatcat caacacggcg tccttcgtcg cggtcatggg
     9781 ggcggccacc tcgcagatct cctactccgc ctccaagggc ggtgtgctga cgatgacccg
     9841 tgagttgggc gtgcagttcg cccggcaggg catccgggtc aacgcgctgt gcccgggccc
     9901 ggtcaacacc ccgttgctgc aggagttgtt cgccaaggac gccgagcgcg ctgcccggcg
     9961 cttggtgcac atccccatgg gccggttcgg tgagcccgag gagatggcca gcgcagtgct
    10021 gttcctcgcc tccgacgact ccagcttcat gaccgcctcg acgttcttgg tcgacggtgg
    10081 catctcggcc gcttacgtca cgcccctctg aagcgactga gtaggcgatc gaaccatggg
    10141 tcagccgcgc tgcccggtca ttgggatcac ctgttacgtc gagtcgatcg accgaccgcc
    10201 gtgggtgtcc caacccagtg ccgtgctgcc ccgcatgtat ctcgagcacg tcgcccgggc
    10261 cggggggatc cccgtgatcc tcccgcccgg cgacgagcac ctcaccgacg ccgaggccga
    10321 cgcgcaggcc cgcgaggtgc ttgaccgggt cgacgggctg atcatcgccg ggggagcgga
    10381 cgttcaggcc agcaggtatg ccgccctccc gcaccccacg tcgcaggcgc ctcgcccgga
    10441 ccgcgacgcg tgggagttgg cgttggcccg ggtctcggcg gagaccggca taccgatcct
    10501 gggggtctgc cgggggatgc agatcatggc ggtggccaac gggggcaccc tcgaccaaca
    10561 cctgcctgac acggtgggga ccgacgtgca tctgcagacg gcgggcgtct tcacgcggca
    10621 taccgtgcgg gtggcggatg gctcgcacct ggccggcgtg ctgggagcgg agacggtgga
    10681 ggtgcccacc taccaccacc agggggtggc gacccatccc ggctacgccg ctacggcgtg
    10741 gaccgccgat ggcgtcgtgg aggcgttcga ggacccgcag gcccgcttcc ggctggccgt
    10801 ccagtggcac cccgaggcgg cagaggactc ctcgctgttc cgaagtctgg tcgctgcggc
    10861 gacgacgtgg tccgcatcgc gagacacctt gtccggaagc ggtccggagt gacaccctgt
    10921 acaacgtgac ccacaccacc ctcgccgctg cgcgcaacat gccagctgcc ctcgttatta
    10981 cctgagcgcg acgagaacac tcgtcgcgca gacctcccgc acccgggggg tctttttgtt
    11041 ggtccaggca ccacccccgc cggccagcag cgacgagaac cgaccgatac gaaagtgatt
    11101 ccatgtccac ggccagtccc gagttgcacg tctacgacac gaccttgcgc gatggcgccc
    11161 agcaggaggg tctgaacctt tcggtcgtag acaagctcgc cattgctcgt cagcttgacg
    11221 agctcggggt cggttacatc gaaggcggct ggccgggcgc caaccccaag gacacggagt
    11281 tcttcgcgcg ggcgcagcag gagttggatc tgcgcaacgc cactcttgcg gcgttcggcg
    11341 ctacccgccg agcggggtcg acggctgccg ccgacccgct cgtccgggcc ctgcttgact
    11401 cgggtgcctc ggtcgtcacc ctggtcgcca agtcgcatgc ccgccacgtc gagctggcgt
    11461 tgcggaccac tctcgccgag aacctcgaga tgatccgcga cacggtgacc ttcctgcgcg
    11521 gtgaaggccg ccgggtgttc cttgacgctg agcacttctt cgacggttac cgactcgacc
    11581 gggcctacgc cctggagtgc gtccggaccg cggccgaggc cggcgccgag gtcgctgcgc
    11641 tctgcgacac caacggtggg atgctgcccg cacagatcgc cgatgtcgtc cgcgacgtcc
    11701 tcgactccac gggtgcgcgc gtgggcatcc actgccacaa cgacaccgga tgcgccgtcg
    11761 cgaactcgat ggctgcggtg gctgccgggg cgacccacgt ccaggggacg gtcaacggct
    11821 acggcgagcg cactggcaac gccaacatcc tcaccctcgt ctccaacctg cagatcaagc
    11881 agggtatgcc gttgctcgag ccgcagcggc tgcgtgaggc cacccggatc tcgcacgcca
    11941 tcagcgaggt caccaacgtc ccgccctaca gccgccagcc gtacgtcggc tcgagcgctt
    12001 tcgctcacaa ggccggcctg cacgccagcg ctctcaaggt cgaccccgac ctctaccagc
    12061 acaccgaccc cgcgctcgtc ggcaacgcga tgcgaacgct cgtgtcggat atggccggcc
    12121 gcgcgagcat cgagctcaag gccaaggaga tgggcgtcga cctcaccgat cagtccgagc
    12181 tcgtcgccgc ggttctcgcc gaggtcaagg agctcgagct gcgtggttgg accttcgacg
    12241 cggccgacgc gtcgttcgag ttgttgctgc gcaagcacct tggccaggcg gtgacctact
    12301 tcgacgtcga gtcatggcgg gtcatcaccg acgcccggga agccggcgag gccgtctctg
    12361 aggcgaccgt caagctcacc gcgggcggat cgcgccaggt ggccaccggc gagggcaacg
    12421 gccccgtcaa cgccctggac cacgcgttgc gggtggcgct catgggtgtc taccccgagc
    12481 tggcccggct cgagctcgtc gacttccggg tgcgcatcct cgacgcctcc cacggcaccg
    12541 acgcgatcac ccgggtgctc atcgagacgt ccgacgggac gacgacctgg cagaccgtag
    12601 gggtggcccc caatatcgtc gaggcctcct ggcgagccct cgtcgacggc atcacctacg
    12661 gcctgagccg ccagggggtg tcggcgcgat gagcatcgac cacgcggccc cgccgcagac
    12721 ggcgcacgcc acgcggcata ccgcgcctgc ggccggagtg gggctggtcc tgcggcccgc
    12781 ccgagccgac gaagcgccgt tgctctccga gctcgcgttg cgcagcaagg gctggtgggg
    12841 tcacagcgcg tccttcctgc gggcctgtga ggccgagttg accctctcgc ccgagcaggc
    12901 ggccaaggcg gtcctcgttg aggtcgacgg ggtcgtcgct ggcttccacc tgctggcccc
    12961 gtcgtcggtc ggcagtgatg tcggtgagct ggacatgctc ttcgtcgacc ccccgttcat
    13021 cgggtcgggg atcgggcgga tcctcttcga ggacgctcgg cgctcggctg cgggtcgcgg
    13081 gtggacccgg gtgcagatcg aggccgaccc tcaggcgcgc ccgttctacg agcggctcgg
    13141 cgccgtgcag gtcggcgagc ggctgagccg ttcggtgccc ggccgcagct tgccgttgtt
    13201 cgagttgacc ctgcgaggcg gcggcagctc gcgccccatc ggtcgcgcga cctactggcg
    13261 ctgactctcg gcgcgaccgc ggtgggtttg cctgggccga tccctgggta gtgcccaggg
    13321 atcggggtgt ctttaggctg ctacggggct acctgtaggt cgagttgggc tgcagcagaa
    13381 ggcaatcggg cggccccgag ggccgcgatc ggcacgtctg acgacagcat cgagaagcga
    13441 ccgggctgcg tcaactcgtc cagcagcggg gcctgcgcgg cccgctgggc caagacctcg
    13501 gcgatcccaa gagacagcgg ttcgcccaag ccggtgaggc cgccgccgat gacgatgtgc
    13561 tcgaccccgc cgacgaggac gagggcttgg atggccatgg cgatgccacc gcagaggtgg
    13621 cgcaccgcct ctgcggcctg ggcgtcgccg gctgcggcgg cggcgaacgg gtcggcaagg
    13681 tgccgcgcgg ggggccacat ccgggtgagc cccgagccgg atgcgctggt ctccagacac
    13741 ccggtctggc cgcagcggca gggcactggc gcggccaaag gcaggtgacc gatctccccg
    13801 gcgatccctc ccggcccgcg gacgatctgg ccgttgatca ccaaggcggc ggccagcccg
    13861 gtaccgatgt tgaggtagcc gaaggccgtg tccgggtccc cgccatgcaa ctggaaggca
    13921 ccccagccgg cggccttcac gtcgttgtcg atgacgacag gccggctcaa ggcgtcgctc
    13981 aacgctgggc cgagcggcag agcgtcgacg ccgaggttga gcgcgtgcgt gaccaggccg
    14041 gagcgcggat cgaccagacc cggcatacaa gcaccgaccg aggcgatctg ggtccagccg
    14101 cccaccgcgt cggcgcactc gtgggccacg gccaacgcca cggcaacgac cccgtcgggg
    14161 ccgtgcccag agggcgctcg acgctcggca acgacgctgc cgtcggggcc taacgccacg
    14221 acggcagtct tggtcccacc gatatcgatg cccatcaaca tggctgcacc agtgcctccc
    14281 gtggcgaagt ggtgagaagt tcgaccaacg ccgccgcgac ggacaatgac ccggagtagg
    14341 ttgccacatc gacgtcgacg tcgacgtcgc cgaacggcca cccgcactct acgagaacga
    14401 cggggtgacc ggaggcaccg tatgccgcgg cagcggctcg ggcgggatgg tcgacgccga
    14461 tcccgcggcc gacgaccgcc acgcgagcgt ctgtaggaac ctgccctggg tcaatggtcg
    14521 cgccgagtgc cgccggaccc cagttggtcg ggccgacagc cagattggcg cgggtgtcaa
    14581 cctggacgat cgccactgga ccggtgccgg cccgccacgc agcgaggctg gggctgacgg
    14641 agaacgttcc ggcgatcgtg gcgcttgcca catccgctcg ggcggtgggg gagggcagtg
    14701 ctccggccgc gggctggctg tcgccgaccg cgagtgagtg gacgtcggcc aacgcggcca
    14761 cccggaccgc tgcatccttt agccgtcgag ggtccaagac gcccgcgtcg acggcggcca
    14821 cgatggcgtc cagcactgcg gcatacccct cgggggtggt gtccgacccg aggcagagca
    14881 gatcggctcc ggcggccagt gcgcgcaccg cagcggtcgg gatgccttgc gtcgcactgg
    14941 cgcccgccat gtcgagggca tcggtgacga ccacgccggt gaatccgagt tcctcgcgca
    15001 ggaccccctg caggatgggc tcggagaacg tcgccggggc gctggggtcg agtgcgggga
    15061 cgaggatgtg cgaggtcatc acggttgcca cgcccgcctc gatcgccgcc cggaacggca
    15121 ccagctcgcg ccgatgcagg gtgtcgaggt cggcgaggac gaccggctga tcgaggtgcg
    15181 agtcggtgtg agtgtcgccg tgaccgggga agtgcttggc gcaggcggcc acggactggg
    15241 tcgccaggcc ctcgacatag gccacggtgt ggcgagcgac gacctcgggg tcggcgccga
    15301 agctgcgcgt cccgatgacg gggttgtcgt ccgccgagtt gacgtcaacc acaggcgcaa
    15361 gatccagacc gatcccgtat gccgcgaggt ctgcaccgat gcgggccgcg ctgcgccgcg
    15421 tggacgcgac gtcgtccagc cgtccgagga cggcgttgcc cggctcgggg gagccgctgg
    15481 ggaagtgcag gcgggtgacg tcgccgccct cctcgtccac ggcgatgagc agggacgtcg
    15541 agatgcctcg caggcttcga cacaggcgat gggtctgcgc tcggtcggtg atgttgctcc
    15601 cgtagaggca gacggtgcgc aacccaccgt caacggcttc gtcgatccat gcaggcaccg
    15661 tcgtcccgac aaagccgggc atgagggtgc tcgcagcgag ggcgcgtaac tgggtaggtg
    15721 ccacgtcagc ccttgacggc tccgccgacc aggccactgc tcattcggga ctggaccagc
    15781 aggaagaaga tgatcaccgg cacggcgaca acggtcgacg ccgccatcac ctgaccccag
    15841 tcggtctccc ggctcgcgct ggcctggacg aagccgcgca gccaaagcgg cagggtgcgg
    15901 gattgctcgt cggtcatgat gaccagcgcg atggtgaact cgttccacgc ctggaggaag
    15961 gcgaacacgc ccgacgccac gaggccagga gccaacaggg ggaaggtgat ccggaggaag
    16021 gcttgggtgc gggtcaggcc gtcgaccatg cccgcctcct caaggtcgac cggcacgccg
    16081 gcgacgaatc cgcgcagcat ccacaccgtg aacggcacga cggcagcggt gtagaggatc
    16141 gtcagaccga ggacgctgtt gagcattccg atccccgaca tcatcttgta ctgcgcgatg
    16201 aacagcccct cggccggcag catctggatg agcagcagag ccaagatgaa cgacttgcgg
    16261 ccccggaatc ggaaccgact gatcgcgagt gcgccgagga aggcgatgaa cagcgaggcg
    16321 gcaacggtgc ccaaggtgac cgcgagcgac acgccgaggg cctgcaggaa cccggggtcg
    16381 ctgaacacgg cggcgaagtt gtcgaaggac ccgccgaacg gcaggaacgt cggggtcgtc
    16441 gactggaggg tgacgttgga cagcatcgag gagttgacca tccagtacac cgggaacgcc
    16501 cagaccaggg ccaggagcag ggtgaggacc gtcaacaggg cgcggccgat tctcctgagg
    16561 gtcatcagga ttcctccttc atcaggttgc gcacgtagcg ccagctgatg gcgatggtca
    16621 gtaacaacat gaggatgctg acggcgctgg cggagccgaa atcgcccttg cccgtgccga
    16681 gttggtagat gtaggtgccc agcagatggg tctcgttcgc cacgccgccg gcgtcctgca
    16741 agatcttgac ctgggtgaaa acgcgcaggt tccagatgaa ctgcagcagc aggacgatgg
    16801 acaacaccgg agcgatgagg ggcacgatga tgtgccgaag gcgctgtatg ccgctcgcgc
    16861 cgtcgatctt ggcggcctcc aacacctcgg gggagacctg ggtgagtccg gcgtagatgc
    16921 tcaaggtgac gaaggggacg ctcatccagg tgacgatgat ggcagcgatg atgaggaacg
    16981 aggtcgggtc ggcgagccag ttgtgtccct gagcgtccac gccgaggcga acgagggccc
    17041 agttgagcag gccgcggcgc cagtcgacca accagatcca gacggtcatg gaggcgacgg
    17101 caggcatggc ccaggcgagc agaagggcga cctggagggt gagccgggcg gccttgtgaa
    17161 ccgagttcat caacccggcg agcgcccctc cgatgagcac ggtggcgaac gagacgacga
    17221 ggcagaagac cagggaacga aggacgatgg cccacagcgc ggggtcgctg acgatctcta
    17281 cgtagttggc cagaccgacc cagtcggccg gaacgccgaa ctgctggcgc aggccgaatt
    17341 ccttgaagct ggtgatgatc tgccacgcga ccgggtagcc gagcgcggcg atcaacacga
    17401 tgatcgtcgg agtgatgaga atgtgaggga gccgagatcg gcgccgcacg ggcatgttcc
    17461 ggccttccgc gcaggagcgt gagggtgtga gggtggtccg ggcgggtgtg ggggtctgcc
    17521 ccgcccggac caccgttcgc tcgatctcag tgacaatcga gcggtctgtg gatcaggcga
    17581 tgctcacttg aggttgagca tcggcgtgat cttggcgtcg tactccttcg ccagcgcggt
    17641 gaggtccgcc gcgtcgcgga tcttgccgaa gaactcctcc atgaccttgg ccgactcaac
    17701 ggcggcccag cccggcgcgg ccggggtcag cttggagttg ctggccgact ggatcgtcgc
    17761 tgtggcgaag gcgtcggtgc cgagcgagga gacgtagtcg gcgttggccg ggcccaggcc
    17821 cgccccaccg agcttcttct ggaagtcagc gctgaagatg atgcgcatga gttccttggc
    17881 caggtccttg ttcttgctgg tgttggagat ggcgatgttt gaaccaccgg cgaagaccgg
    17941 ggccggcttg ccgtcgttgc ccggcaggac gaagacgccg aaggtggcgt cattccactg
    18001 acgagttggt ttgccttcct tggttgccag gtcaccgatc gaccagtgcg cccaacccgg
    18061 agccatgatc gtggccgcgg caagcgacgt cgtgccggtg acggccttgg tgtcgtcgag
    18121 gatctggtca gtgtcgttga tgtagagcca cggcgaggca tccttggcgt cagccggcgc
    18181 cttggaggcg ttgcggtaga tgtcttgcaa ctgggtcagg cccttgatgg tgttggggtc
    18241 cgacagggtc gacacccact ggccactttc cttcttggcc aggtcgccgc cgttggcgaa
    18301 gacccacgag atgccgttgc gccagtcctg gccaccgatg aagaagccgg agaagttggc
    18361 gatcttgcgg gggttggcgg tggtgatggt cttcaccgcg gtgttgaact cgtccagggt
    18421 cttgggcgtc gcgacgcccg cctgggtcca gacgtccttg cgctggaaca ggtaccggga
    18481 accgaagtag tacggcaggg cgaactgctt gccgtccacg cttccggcct cgacgaacga
    18541 ggggagcagc ttggcgccgc cgagttcgtt gtacatgtcg ctgatgtcca ggaaggcgcc
    18601 gacgttggtg aatgtcgggg actgagtgtt gcccagctcg acgacgtcag gggtgttgtt
    18661 ggcgtccggg agggcggtgg tcaacttggt ggtgaggtcg ccccaggact gctcctggat
    18721 cttcagcgtg gcaccggtct tggccttgaa cgtgtcggcc aggtaggtgc gcaattcggc
    18781 gggggtgtcg ccgccggcca gccacagggt gatcgacttg ctggcgttgg cggccgtgtt
    18841 ggtcgcggtc ggggccgacc ccgtgctgcc gccactacag gccgtgagca gcaacgtcgc
    18901 tgccgtcccg accacgaggg cggcctggag gttcttcttc atgggtttcc tttcccggtg
    18961 ggtgggcccc tgtgggggcc ggttccctgg ttcggtccta ctgttctttc ctcggatgaa
    19021 tcgccgacat cggtgagggg tacgacgtca ggcacgtgcc gatagggctg gctagctcac
    19081 ccccagttgg tccagcagga ccagggcggc agcaccccgc acgacgatgt cgttggggtc
    19141 ctcgaccacg cgaacccgaa gggtcgcgtt ctcgacggtg aagaggcggg cgcgcagcgt
    19201 gcgctcgacc gactcgacca aggccggcac gatgacgtgg gcgggaccac acagcaccac
    19261 gtcggccagg tcgagggctg cgacgaccgg tgccagggcg atcgccagac gctcgcccgc
    19321 cgcggtcacc gcatcaggga cgtcatcggc gtggggagcg gcgtcagcag ggtcgggtgt
    19381 cgacgcggcg atggcctgct cgaggcgggg tgcggacacc cacgtctcca agcaaccgac
    19441 cttgccgcac cggcaccggc gtccaccgtc ggtcccgacg acgacgtgcc cgatctcacc
    19501 ggccgccgag tgcgcgccgc ggacgcggcg gccgccgacg atgagcccgc agcccacacc
    19561 gcgaccgatc ttgacgagca cgaggtcgtc accgccgtcg ccgagggtgt ggtcggcatg
    19621 ggccgccgcg tcggcgtcgt tgcacaggga gacgggcagg ttggtggctg acgcgacgat
    19681 gtcgcgcagg ggaacgtcgc gccagtcgag gttgggggcg gtcttgatca cgccgtcgct
    19741 gcccacaatg ccgggggtgc cgatgccgat ccccaggatc ggcgcggtcg ccagcgccag
    19801 acactcggcg acgagttcca gcacgaggtc gactgcgcgc tctccgccga gcgggcccgc
    19861 gggacgatcc ggcccagccg tgctgccgtc cccgatgacg gagtcgtcgg ccaggacggg
    19921 ccgttgggca cgagcgacga cggtgccgtc gagatccagg acggcgccgt gcagcgcgcg
    19981 gtggtcggag aggtcgaggc cgatcatctg caggccggtg cggttgacgt cgaccaggat
    20041 cgagggcttg cccatccgtc cgggggtgct ccgcccgacc tcgatggcgt ggccgcggtc
    20101 gaggagggcg gtgaccaact cggagacggt caccttggtc aagcccaatg agcgagcgag
    20161 gtcggcgcgg ctcatcgggc cgctggagta gagggcctgc agggcaagcg agaggttctg
    20221 gcggcgcgcg tgactgggct gcacgggcgc tgacggccgg actcgctgtg gcctcactga
    20281 actcatgtta gttagtaaac actactaacg aagtaagaac aagtcccagg ggtgagaggt
    20341 gggtctcgta gccctggcgg ttagtcgatg gaggcgcgct cggccagggc gctcgcgcag
    20401 gccgacagca ggcgcatccg cagcgcttgg ccgcgcgcgg cataccggcg ttgttcgccg
    20461 gcgtaggtgg ccttgccctc gggagtctcg acggcgatcg gctcgatgcc caggtcgcgc
    20521 aggtcgtagg gggaggcgcg catgtccagg acgcgcaggt ccagcgcgag ctccaaggtg
    20581 tcggcgacca gctcgctggg gatggcgggc gcgagtttca tcgcccactt gtagaggtcc
    20641 atcgttgcgt gcaggcagcc gggctggtcg agatcggctt ggctcgcccg ggtcggagtg
    20701 agcaggttga gcggccgagc cggttcggtg aagaagcgga aggcgtcgaa atgactgcac
    20761 cgcaacgggt tcgcttcgac aacggcgtcc gttcctgccc tcccgagacg caacggccac
    20821 cgggagtggc gaacctcgtc ggggcgctgg cggtagacca tcgcccactc gtgcagaccg
    20881 aaacacccga gttggggagc gcggtcggcg gtgcgggtga gcaggtcgcg tacgaaggtc
    20941 agcgtgtcgc cgcgggccgc gaggtatgcc gcgacatcga gttccacgcc gtcatcgacg
    21001 atccggtagt gccgccactg ggcacgcggg tcagcggccg cgccagcgag ccgcacaccc
    21061 agcccggggt gccaccgccg gatcgccgac ggcgtgaacg ggtagtaggt gaagacgaag
    21121 tcctccaccg gatgaggtat gccgcgtgct cgcctttcgc gatgaccggc agtgagcgcg
    21181 tcgacccgat cagcatgagc cgccgcggcc gcccgccact cctgctctgc cagcacctgc
    21241 gtcacggcac ggcagcctag tcgccgtgag cggacggcag tcgttctaga gtggcccggt
    21301 gcggatcgcg agatacacca caggtgagga cccggcatac gggatcgtcg acggcgagcg
    21361 cggcgcggag gtgatccacc ccctcaaagg cgaccccctc tatgtcggga tcgtgcccgc
    21421 tgggctcgag ccgatcgcgt tggaggaggt gcgcctgctc gctccggtca tcccgcgcag
    21481 caaggtcatc ggcatcgggc gcaactacgc cgaccacgcc aaggagttcg gcaacgaggt
    21541 gcctgccgag ccgatgatgt tcctcgtccc caacacggcc gtcatcggcc cgggcgaccc
    21601 ggtcgtcatc ccgcggggga gtgcgaccgt cgactacgag ggcgagttgg ccctggtcat
    21661 cggcaagatc tgcaaggacg tgcggcccga gcaagcgatg gagtgcgtct ggggctacac
    21721 ctgcgccaac gacgtgtcgg agcggacctg gcagcgtggc gacgggcaat ggtctcgcgc
    21781 caagggcttc gacacctcaa agccgttggg gccgtggatc gtcaccgacc tggacccgtc
    21841 ggcactgcgg ctcgtcaccc gtcgcgacgg agaggttgtc caggacggga cgaccgcaga
    21901 cctcgtccat gacgtcgcga cgctggtgtc ttacgcgtcg acttacttca ccttgctgcc
    21961 cggcgatgtc atcctcacgg gcacgcctgc gggtgtcggg cccgtgcaag cggggcagcg
    22021 ggtctcagtc gagatcgagg ggatcgggac gctcgacaac cccttcgtac gtcgctgacc
    22081 gtcgccaccc cagccggcgg ccgagcacgt cagtgggccg cgttggccgc atccttcttg
    22141 cgggggatca ggtgcatgat cagcacgacg acggcgccga cgatgagccc ggcgatggcc
    22201 gagcccagcg tgttggtgag ccaggcaagg gcgccgcttc cgaccgacac ggcagctgcc
    22261 acagcctcct ccgcgtgatg gaccagggcg taggggccgt gccagccgag ggtgtcggct
    22321 ccgacgagca ggatgtggcc accgacccac aacatggcga cggtcccgat gatggccagc
    22381 cagcgcagta ggacgggcat ggcggcaacg aggccacggc cgatcgctcg cgctgcgccg
    22441 gactgccgct cgctcaggtg cagaccgatg tcgtccatct tgacgatgag ggcgacgaag
    22501 ccgtagaccc cgaccgtgat gccgagggcg acgacgatga ggatgatcag tcgcgagagg
    22561 aagggctccg tggcgacctc gttgagggcg atgaccatga tctcggcgga gaggatgaag
    22621 tcggtgcgga tcgctccggc gaccatcgtc ttctcgtggt cctcgccctg cacgatggcg
    22681 ggggcttctt ccccggcgtc gtggccgctg accttctccc agatcttctc ggcgccctcg
    22741 aagcacagat aggtgccgcc gaccatgagg aggggggtga gcgcccacgg caggaactgc
    22801 gagagcagca tgatcgccgg aaggatgaac accagtttgt tgcggatcga cccgatggtg
    22861 atccgcttga tgatcggtaa ctcgcggtca ggagtgaatc cctggacata gcgaggggtg
    22921 acggccgtgt catcgacgac gacgcccgca gccttgacgc tggcccgccc cgcggccgct
    22981 ccgacgtcgt cgatcgaggc tgccgccagt ttggcgatgg ccgcgatgtc gtcgagaagc
    23041 gcggccagcc caccgctcat cggtgtgccc cgcggccgtg ctgcgctggc gttccgtgct
    23101 gattcacacg gacagactag ttggctcgcc gcgcggctag gctgacgtcc accatgagtg
    23161 agaagcagct accgggcgcg gttcgtctgc gcgtcgcccc gtcgcccacc ggcgatcccc
    23221 acgtcggcac ggcatacatg tcgatgttcg atctcgcgtt cgcgcgccaa caggacgggc
    23281 gtttcctcct gcggatcgaa gacaccgacc gtgcgcggtt gcaggaggac agcgagcggc
    23341 agatctacga caccctgcac tggctggggc tgacgtggga cgaggggccc gacattggcg
    23401 gcccgtgcgc gccctaccga cagtccgagc ggttggagac ctaccggccg ttcgtggagc
    23461 gactgctcga ggcgggccac gcctatctat gttggtgctc cgccgagcgg ctcacggcaa
    23521 tgcgtgagga ccaacaacga cgcaaggtgc cgaccggcta cgaccgactg tgcctgggca
    23581 agacccgcaa ggaacgcgcc gccctgcccg gcttctccga gacgccggtc gtgcggatgc
    23641 agatccctga cgacgtccag acctccttcg acgacctcat tgccgggcgg atcgacgcgc
    23701 cggtccctga cgaccaagtg atcctcaagg ccgacggctt cccgacctac cacctggccg
    23761 tcgtcgtcga cgatcacctc atggggatca cccacgtcgt gcgcggccag gagtggatct
    23821 cctcgacgcc caagcacctg ttgctctatc ggtggctggg catcgatccg ccggctttcg
    23881 ctcacatgcc gttgctgcgc gacgagcgca aggcgaagat ctccaagcgc aagagcccct
    23941 gggcccggct gacgtggttc cgggagcagg gctacctccc cgaggccctt gtcaacttcc
    24001 ttgccctgca gggctacccg ccgatcgtcg aggcggatgg gaccgagcgc gaggtcttca
    24061 ccttcgacga gttcgcggcg cgcttcgagt ggtcgaaggt caaccctgcg gggtcgatct
    24121 tcaacctcga caaactcgac tggctcaacg gcgtgtggat ccgcgagttg ccggtggggg
    24181 agttcgcctc ccggttgctg ccgttcctcg aggccgacgg gatcttgtcg gacaacccgt
    24241 cgctgggcga gttggctcgg ttgcggtcca tcgcggcgct catccagacc cggatggtca
    24301 agttgactga ggccagcgcc ctcgtggcgc ccttcttcat gggggatgac gtcatcgccg
    24361 tcgccgacga cgcgcgcgcc caactcgatg cccagtcccc ggcggtcctg gacgctgcga
    24421 tgcatgccct cggcgacatc gacgaccatc agcggggcgt gctcgggtcg gagtccgatt
    24481 ggagcgccgc tcggatcgag gctgccctgc gttcggcgat cgtcgaaggt ctcggggtca
    24541 agcccaaggt ggccttcggg ccgctgcgca ccgcggtttc gggtcagcgc atctcgccgc
    24601 ccctgttcga gtcgatggag atcctcggga agacctccac cctggcccgg ttgcaggcgt
    24661 tgcgagacac cgtctgaccg gggatttggg cgaggctctg ctgggccggt agtatcgctc
    24721 ttcggttcac tttccgatcg cttccgggcg aacgggaggt ataccccatg gggtatggtg
    24781 taattggcaa cacgtcggat tctggttccg caattctagg ttcgagtcct ggtaccccag
    24841 cttcggccgc ccagtgggcg gccgattcgg tggaagcccc cggtcaccgg taaggtttcc
    24901 acctgtgtcc gacgaggaga cccctcggac gacatcgcta gggccccgtt gtgtagcggc
    24961 ctagcacgcc gccctctcaa ggcggtagcg cgggttcgaa tcccgtcggg gctacgcgcg
    25021 agaacggccc ctgaccgaca catcggtcag gggccgttct gcatgtccag gcaggtgggt
    25081 caggccgccc agtgggtatg ccggtcagcc cagttcgcgc agccgcggca ggaccgactc
    25141 cgcaaaggtc gtcaggaacc ggtgctggtc gtgtccggga gcgtggaaga ccaggtgatc
    25201 aaagccccag ccgacgtagg ggcggaccgc ctccacgacc tcgtcggggt cgttgctgat
    25261 gatccagcgc cgagccacct gctcgtcgct gagctcgtcg gccagccgct ccatctcgat
    25321 cgggtcggtc gtgccgtgct tctgctcggc ggtcagcgac agcggcgccc agaaccggca
    25381 gttggccacc gcggccgcgc gatcggggtc ccaggagagc ttgacctcaa tcatccggtc
    25441 gtagccgacc ccgtcgcgac cggccttcgt caggccctcg tcgacagccg gcagtagttg
    25501 gtcgcggtag agctcctcgc ccttcccgga ggtgcagatg aacccgtcac ccgcgcgacc
    25561 ggcatatcgg gcgaccagtg gcccgcccgc ggcgatgtag acggggacgg ggacctcggg
    25621 ccggtcgtag atcgtcgcgt cggtggtgcg gtagtagtca ccttcgaagg tcacccggtc
    25681 ctcggtccag agggtgcgga tgagcgcgat cgcctcgcgc agccgggcga agcgctcctt
    25741 gaactccggc cactcggcaa ccgcgcccgt cgcgaactcg ttgagcgcct cgccggtgcc
    25801 gacgccgagg gcgatccggc cggggttgag gacaccaagg gtggcgaacg cctgagccag
    25861 cacagcgggg ttgtagcgga aggtcggggt gagcaccgag gtccccaact ggactcggga
    25921 ggtgcgctcg cccgccgcgg ctagccaggc cagcgagaag ggtgcgtgcc cgcccacgtg
    25981 acgccagggc tgaacgtggt cggagaccca gaccgagtcc agcccgacct gctcggccag
    26041 cacggcgagc tcgacgagct cacgcggacc gaactgctct gccgacgcct tgtagccgat
    26101 ccgcatgcca actcccgggg tgacgtggcg gtggaggccg ccgcacggca tacccctgcg
    26161 gtatgccgtg agcgcacctt accggcggcc gcgccggtgt cagcggtaga cgacgacgag
    26221 atccccgacg cgcgcttcgt tgaacagcca ggccatcttc gccttgtcgc gggtgttgac
    26281 gcacccgtgc gagcccacgc cggagtagcc gatggaggcg aactcggggg agtagtggac
    26341 ggcctcgcca ccgttgaaga agagcgcgta gggcatgggc gcgtcgtact tcttggagta
    26401 gtggtcgatc gacttccagt agatgtggaa ggcgccgtca ttcgtcggca actccgcccg
    26461 cccgaaccgc gcgtccatct gcatgacggg aacgccatcg acgacccaga cgaccttgcg
    26521 cgtcgacttg gacgcgcaga tcactcggcc gaccaggcag cgcgggtcga gcccggcggt
    26581 ggtcagcccg gcacccggcg cgggcttgac gttgttgagc tcgttgctcg tgggcttgcg
    26641 ggtctgcccc cagatgcggt ccatcgtgcg ttggtcaacc tcgcccgtca ccggaaggcc
    26701 gcgcttggtc tggtagccct tgaccgcgct aaccgtcttg gtgccgtagg agtcggtgac
    26761 atcgccggtc caccggccga gctgcttgag ccgggcctgc aactcccgga ccttggctcc
    26821 ggaggacccg gacgcgagga tggccggccc ggcgatgaga tgcccggcca ggtcctcggc
    26881 cgaaggagcc ttggtcgtcg ctaggagagt cgaccaggtg gtctcatcga ccgatccgga
    26941 cgccgggagt ttgagggccg tctggaaagc ctgcaccgcg cccgtggtct cggcgtcata
    27001 gacaccgtcg acggtggacg tgaagttctt gagctgcttg agccgggcct ggagctcgcg
    27061 cacctggtcg ccgtcggcac cttcggccag cagcggaggg acggcggtcg gcgtggcggt
    27121 ggctgtcggg gtcgccgaag gggtcggcgt gacggacgtc tgcgccgcca cgggggtgac
    27181 gacggcggtc gccggtgctg cggcgggcgt cggggctgcg gtgcaggcgg aggcgacgag
    27241 cacgcccgcc agcgagatga tccccactcg cgcggctctg ccgatacgtc gtgtcactct
    27301 tcgtctcctg ccaagcgtgc cgaactgctg ctctcatgac gccccaggtc tccgacaggt
    27361 tgatccggtc agggtgaaca accggagcga cgagcggtct gccagggcgg catacctcaa
    27421 ctcagactag ctcggtgtgc tctggtgcgc ccggcgcaac acttcggtga gtttgttcgc
    27481 gccggcgacc acggtcgccg cgtgcaagcg ccccggctgc cgggagagcc gctcgatcgg
    27541 ccccgagatc gacaccgcgg ccaccacccg accggacgga ccgcggaccg gcgccgagac
    27601 cgatgccacc ccaggctcgc gctcaccgac gctctgcgcc cagccgcgac ggcggacggc
    27661 cgacagtgcg gtggcgttga aacgcgcacc ctgcagcccg cgatgcaagc ggtcgggctc
    27721 ctcccacgcg aggaggatct gggcgccgga gccggcgagc atggacaggc tcgccccgac
    27781 cgggatggtg tcgcgcagcc ccatcggacg ctcggcggcc gcgacgcaga tgcgcaggtc
    27841 gccctgacgg cggaagagtt gggcactctc gttggtgtgg tcgcgcaagg cacccagcac
    27901 cgggcccgca gcggcgagca ggcggtcctc gccggcggcc gacgccagct cggccagcct
    27961 cgggccgagg acgaaacgcc cctgcatgtc gcgcgtcacg aggcggtggt gctcgagcgc
    28021 caccgcgagc cggtgggcgg tcggtcgagc cagcccagtc gccccgacga gttgggcgag
    28081 agtggccgga cccgcttcca acgccgacaa gacagtggca gccttgtcca gaacgccgac
    28141 cccgcttgag ttgtccatgc actgatattg ccgtctcagg gcgtgagacg caaatccacc
    28201 gtgcggaacg gtgtcggatg ctggcatcga caggcgtatg aagatggctt gggaaggtgg
    28261 cgggatggcc gggacgctgg ctgagaaggt ctgggaggcg catgttgtgc gccgcgctga
    28321 aggcgagccg gacctgctct acatcgacct gcacctcctg cacgaggtga ccagcccgca
    28381 ggcgttcgac ggccttcggc ttgagggccg caccgtgcgc cggaccgacc tcaccctggc
    28441 gaccgaggac cacaacgtcc caacgacgcc gggcccgatc accgatctca tcagcctcac
    28501 ccaggtcgag accctgcggc gcaactgtgt cgagttcggg gtgccgctct atccgatggg
    28561 cgacgtcgag cagggcatcg tccacgtcgt cggcccgcag ttggggctta cccagccggg
    28621 gatgaccatc gtctgcggtg acagccacac ctcgacccat ggtgccttcg gcgcccttgc
    28681 cttcgggatc ggcacctcgg aggttgagca tgtgctcgcc actcagaccc tgccgctcaa
    28741 gccgttccgc acgatggcag tcaatgtccg cggcgccctc cccgacggag tgaccgcgaa
    28801 ggacgtcgtc ctggcggtca tcgccaagat cggcaccggc ggtgggcaag gatacgtcct
    28861 ggaataccgc ggcgacgcca tcgagtcgtt gtcgatggaa gcccggatga cggtctgcaa
    28921 catgtcgatc gaggctgggg ctcgcgccgg gatgatcgcg cccgatgaca ccaccttcga
    28981 ctacctcaag ggccgcgacc gcgcgccggt gggtgccgat tgggacgccg ccgtcgaggc
    29041 gtggcgccag ttgcgcagcg acgacgacgc cgtgttcgac gccgaggtcg acctggacgc
    29101 caccaccctg agcccgttcg tcacctgggg caccaacccc gggcagggcc tgcccctgtc
    29161 ggcgagcgtt cccgacccgg accggatcgt cgatgaaacc gagcgcgccg acaccgagcg
    29221 cgccctggcc tacatgggcc tcgagccggg gacgccgctg cgcgagatcc ccgtcgacac
    29281 cgtcttcgtc gggtcgtgca ccaacggccg gatcgaggac ctgcgggcgg cagccgacgt
    29341 gcttcgcggc cgccgggtcg ccgagggggt ccgaatgctt gtcgtcccgg gatcggcgcg
    29401 ggtgcgggtg caggccgagg ccgagggtct gcactcggtc ttcgaggccg ctggcgccga
    29461 gtggcgtctg cccggctgct cgatgtgttt ggccatgaac cccgacaaac tcgccgccgg
    29521 cgagcgcagc gcgtcgacct cgaaccgcaa cttcgaaggt cgccagggcc ccggtggtcg
    29581 cacccacctg gtgagccccc tcgtggccgc agccaccgcc gttcgcggca ccctctcctc
    29641 gccggccgac ctggacaagg actgagcgca tggacgcctt cacgacgcac accggcatcg
    29701 gcgtcccgct gcggcgcagc aacgtcgaca ccgaccagat catccctgcg gtctacctca
    29761 agcgagtgac gcgcgacggg ttcgaggacg gcctcttcgc cgcctggcgc accgacgagt
    29821 cgttcgtcct caacaacccg cggtatgccg cggggtcggt cctcgtcgcg ggtcccgact
    29881 tcggcaccgg gtcctcccgc gaacacgccg tgtgggccct gatgaactac ggcttccgag
    29941 ccgtgctgtc ctcgcggttc gccgacatct tccgcggcaa ctcgggcaag cagggactgc
    30001 tcacggctca ggtggcccag gacgacatcg agctcatctg gaagtacctc gagcaggagc
    30061 cgggtgccca ggtgaccgtc gacctcgcgg cccggacggt gacggccggt gacatcgtcg
    30121 cgccattcag cgtcgatgac tacacccgct ggcggctcat ggaggggctg gacgacatcg
    30181 gcctcaccct gcgccacgac gacctcatcg cgcagttcga agccgaccga ccggcatacc
    30241 tccccgtcac gagctgaggc gctgcggtct ctcggacgaa ccgggcgaag cgtgcgacac
    30301 gccgagtcgg cgtgtcgcac tgggagatac gccacacgcc tgcgtggtcc acgcaatggc
    30361 cccgtgcagg gtggcgacgc cttcgcttcc gctgagatct gctgatcggg tgcccaaaag
    30421 cgttgctgga caacctcttt gggggaagcg cggcgtcgtg gggtggccaa agcgagttca
    30481 caacttgtga gcacgcgtcg aaaactccgc ttcgtgagag caaatacctt gcgcggcaac
    30541 gggaatcgca acccgagatg gtggactcca ggcctggacg ggaataccgt cgtccaaggc
    30601 ggacgacccg tccgcgatat cgacacccgg ctggatccgg ggcgttcatc tggaggggaa
    30661 gcgtgaacaa ggcagagctc gtcgtagcac tcgaagacaa gctcggcggc aagaaggccg
    30721 ccagcgatgc actcgacgcg gtgctggacg caatcattcg tgaggtcgcc aagggcggca
    30781 aggttgccat caccgggttc ggtacgttcc agaaggtggc gcgggccgct cgcactgggc
    30841 gcaacccccg cacgggtgac gccgtccgga tcaagaagac gaccgtcccg cggttccgcc
    30901 cgggcacctc gttcaagggc tacgtcgccg acccgcgctc gctgcccaag gccgctccgg
    30961 ccaccggccg ggccccggcc ggctcggttg ccaaggctgc cccggccgct gcgccggtga
    31021 agaaggccgc accggcc
//
LOCUS       DAOMZY010000007        30703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_1154_length_30703_cov_6.243605, whole genome shotgun sequence.
ACCESSION   DAOMZY010000007 DAOMZY010000000
VERSION     DAOMZY010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 30703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30703
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1154_length_30703_cov_6.243605"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3244
                     /locus_tag="PLK64_00910"
     CDS             <1..3244
                     /locus_tag="PLK64_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78328.1"
                     /translation="TDRVTVSVLDVISGSGYLMIHGNGVAIPGLPAVSSGLGIATGFH
                     ISAVLMGSKAIGLYLEVAAGFDAIVGFDPFFIAGKIYARGELRLFIISIGASAELTVM
                     VGKRVVGGVVEDQPYVHGKVCGSIDLFFFEIKGCVSLTIGSEPDDDPAARPLVAGVSL
                     VSRSPALVEGSGTGRAIDGKLGDALDTTAPAAPLITVPLDAFPVITFDAAPTVGPNAV
                     MGANALGQTGAGANPWTRIGERWWRYEVLDVQLTGALIPATGGKTPSSWWTGRPPGQP
                     VPTTALALLNWLPTPFSRAIPYGEELIRSVRDRWGTVCHPAAPSAPLLWTFADSPWGP
                     NPPGWLLDGIAWPDPPETVRSAPAQGRLHVTEPWRIPERPDLDVLQGTEPARVIGDHV
                     PCTKRGGDTPTRAAMTRMTTSLARDLGTGPSAGSSAFGEAAYGQAMEALATGTSLGDL
                     AAHHATTAWAPETTGAQCRGAILRSPVDDVPEPAPHGSPLDRDIVKRGWADSGFKPDD
                     LRDAVRLTPSEPALELSALLLVPTRALEGGIILRVEDPAGNEILQRPLTGADVVGTGN
                     PLPPALTDPNGPWFDPTLRAARIGSRILADDKSYVAVWVSLRKLDAEIGSVVIGWREG
                     AFGGKEERPFYVVAVTAVLGSEAWRESWDTTTITEDRDALQSSLTQDPDDHALLSPGT
                     TYTVAVTWRAASVKQDSQPTATTTPDWQANETQSYRFATEPASASPTDLGPWVLASAP
                     GMGDVGVFCTEPVRIALAHQQVADLFGAYGKELRVVVRSASGKHPAPPDGGPPGAAWT
                     LPLGPSEFLAPALALKIATPWSEAVATMLQTSDLPCIDISGTKDHTVTITLPYTFEPL
                     TDYLIDVHAVPVGAPASATGLVHRIPFTTSRFESLEHFVSYIAPASVRARLVPSPAAL
                     AALPGQPTGDQVDAAYQGAGLGVPTTPSFPAVEVLWSGDPVPQPVAIIVESSEPLWRS
                     RVMPQQVTGPVDAADPLHHWWAGVTGDWLSLAPSTAPVAAGDLPRAGITRIVACPGRT
                     RAVVLLGAGARGAEARLDLVLADDDLAGLPERRSTVVRVSLQRAPWEVED"
     gene            3244..>3937
                     /locus_tag="PLK64_00915"
     CDS             3244..>3937
                     /locus_tag="PLK64_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78329.1"
                     /translation="MTRVSAFSLFRAEATGLPWAQIGVRALPVGQHAPDGWLDELKKR
                     TAERNNDVHVWWACSPTLGVPRGPFTVWVGSPSLKQVKDVDFTSSQRGGGLLLRWGGD
                     EAAFIVVDFDPINPNAAVGLFAHRTAAAMADVVAVSTAPAPGPVHVQLVIKCSGATSA
                     LLVNGMNAKVRIRTVEDVINDDTWKPIELVGLPCEQPWGATAYDSSDQGLVAAPVPPF
                     EAAVQRLKRGMPP"
     assembly_gap    3938..4037
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4038..7052
                     /locus_tag="PLK64_00920"
     CDS             <4038..7052
                     /locus_tag="PLK64_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78330.1"
                     /translation="PVPPFEAAVQRLKRGMPPLGWPPMTQTGRLAPDWVDPDPELVVK
                     EVVQSLLPEIAELYDPGVPERLQFTLSSDRAVPGPTQDGRTSALDTSATTQPLAMLAL
                     PAQTDPVLNLATGFGTTYTREGRQDGAVVGHQDFLVTADYERTPSPWPDKVTFAAYAP
                     QTGPHEIVPPPTALTAERNGLVPPNPADTPWRESIRVSWNRLPTTAAMGRVGAGTLAR
                     YDAGAAQATSLAPKRDADGIRPLTLSPDGAPGTPGSGRVAFVDGAADIPILSGGRQVG
                     YAVSLVDVHGVWSAWRDIAYTGNEPGPLPPRLIALDLASTYAGSPACPATLTLETALD
                     WTERRPTSLQIVALFFPMASASTPPPAGLDPGLPTPAGCFRRDLGLTFAGDVPTGVGA
                     TVTSLGPDGANPMAPGPGQGDGGRRYRLTSGVPTLDFTSTQRWGVQVWLRSTLAVGAS
                     PTAYTPDAAHPAVTTAASPVPIVPLPPPAPPGVPMGSTPDAQGSSHVRVQWSLPAGGT
                     GVRSCIVWECSETALRQRAGLPPRAPETDSPGVRLAALWTAYDALTPTQQRAAFRRLL
                     TLPGGARDADVTLPKGSTDIHLFTVTTQSETGIESPWPGPAPTGHIHLQAATAPRLRR
                     PTPPLTRVSVAPDGTVTVRLFSASRVPVARFLVYATRSEAAARDHESMGPPVAVVPVV
                     LPPGGPDPVTGAPVYEATWTGTLPPAWDNWLVRAVAKPVDTVPVQAVRGQPSAASEVV
                     SILVPPTGPPDLDPLVASRWGADHRGVVVTSGTSGVARVTSAGVFRLGGVAEAMPVAM
                     TDLPLLPETPGTSAPAGATSSPVLERGPRTAGRLPLRLWIRTPDATAPATVSMRLLDP
                     MGRSVERTVTVPGFVPTPAYTLTIVRTSTVASGVVLAVGTDAPATVSAGISLSVRGVS
                     TDLTPLPLPFPRPRPGLLRVSGTFLLSGVRTGRITFPADGQIHVIRPQRTLGWPREAR
                     YAVFVPLVSPVRITLMLLGPAGEQLVTVTGGA"
     gene            7122..7988
                     /locus_tag="PLK64_00925"
     CDS             7122..7988
                     /locus_tag="PLK64_00925"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HPV78331.1"
                     /translation="MTTPTKAPSIVLSPRLGGGRTLESVARRCLEHTDANEPLLDAHG
                     DHEALHQFRVGYRRLRSLFSLAREVSRADPEARRLKAQLRELTVAFGPARDLDVFLEG
                     HPDLSPTNAARLEDARVTAYSEALEFLHSPEWAQTRVDLDEWLRNGSYRDLLGTGTWS
                     GRAWTAGALERRRKRIRTMSRRLADLSDHDRHQLRIEAKKVRYGCQFFGTMWPRTASD
                     ALVMEKTLGALQDRLGALNDLATWSRLVDLTGVTDLLPPEVDVEAELEAAQELADRIA
                     QLPGFWEATLQK"
     gene            8325..9206
                     /locus_tag="PLK64_00930"
     CDS             8325..9206
                     /locus_tag="PLK64_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78332.1"
                     /translation="MIDTGSNRFSGELRVALHASGLTLDAVQRRLAERGFAIGRSTLS
                     YWQNGHRLPTQPTSLLAVTALEEILKVPIGSFTEALRHPNSTPVSPELDMAAAAARIG
                     VLMEGVGCPDGMATLEPLSAIQVAEFGPTGCLRSVRAVETYRALVDTDRFPILHGGEP
                     GGRPELIRHEAVSGGRIGRVRRDAEANVVVSELVFDRFVRRGEHHFLHYLTFDDNEQR
                     SLLLYRLVVTSRSMMVLDLGFHPDCLPVRLEEFERATDNGPDLFVRQRTLSPDRRVTL
                     VRERARRGIVGLRWEYA"
     gene            complement(9254..9781)
                     /locus_tag="PLK64_00935"
     CDS             complement(9254..9781)
                     /locus_tag="PLK64_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78333.1"
                     /translation="MSGSRAQLLRYAATWVLVVSVTAGLSWGAISRAGEAATLLGAPV
                     PLGSAAAPTTAGLTPSATQSVPPPPPVTSAPTSSGQSSTSPSSPSTPKPEPTESPRPV
                     VRSVSTAAGQVWAYCVGDTAYRRSVVATDGWSFEFSREDNGRFEVKFQLAGAEEEEVE
                     VKVRCEAGLPVFTTD"
     gene            9817..10521
                     /locus_tag="PLK64_00940"
     CDS             9817..10521
                     /locus_tag="PLK64_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPV78334.1"
                     /translation="MARVLVVEDDPAIRGALLRSLAGAGHAVTSQPTGMAGLTEAVDQ
                     PPDVVVLDLGLPDVDGLTVLSMLRAVSSVPVVVATARDDEKEIVRALDLGADDYLVKP
                     FGGDQLEARIRAVLRRAGHDAPPTPIVVGGLVITSASREVTVDGIPVELSRKEFDLLH
                     LLAQRVGEVVTKREILTEVWRQSYGGGDRTVDVHLSWLRRKLGETAADPVYLHSVRGV
                     GVRLVDPRGAHPGSAD"
     gene            10521..11939
                     /locus_tag="PLK64_00945"
     CDS             10521..11939
                     /locus_tag="PLK64_00945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPV78335.1"
                     /translation="MLRSRITWLAAALVAGVVISFVIPLCLLVRTMAEERAVSATNSD
                     AQSVAMIVAGVDDPASLPAVLAPTLGARTATTTVALPSGQLIGGPTTTGPVTTGQPSS
                     GPAQFPEDARLLAAVRADNRSMATTVDDRFHVLIPVGVADGVAIVHSVTDLGRVRRGV
                     YGSWAILVAVGLLLVVVSVLVARRQSARISTPVVAMAGVAHRLRSGDFEARAAATGPV
                     EVVELGHALNQLADRIEDLLRIEREGAADLSHRLRTPVTALRLDTDQVEDPTVRARLR
                     SHVEDLHRAIDSVVADARRPSRDAMGGWCDARVVLADRAHHWGPLAEDQGRMTTVIGG
                     SANGSASGAAGESVTGEHPGGEHAAYRPALVGLAARDLADLLDNLVDNAFAHTPEETP
                     IRLELHVVDGLVVLRVEDGGDGSPDTVAFARGESGAGSTGLGLDIVRRLAADAGGSVG
                     FERAGLGGLAVIVVLPLIERAD"
     gene            complement(11983..12402)
                     /locus_tag="PLK64_00950"
     CDS             complement(11983..12402)
                     /locus_tag="PLK64_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78336.1"
                     /translation="MWIGWKAISLGVVSGGAIAVALYSSLAAGSAAPSASIPISQTVP
                     TAVYADCVEPAVLEGDECVTHVSVTRTVAAPAPAAVPVPAVAASSATTSATRYTPAPT
                     PEAAPAAATVDDHDDEYENERDGDDEPDDDERDGDDD"
     gene            12697..13086
                     /locus_tag="PLK64_00955"
     CDS             12697..13086
                     /locus_tag="PLK64_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78337.1"
                     /translation="MTAMHALTGVVAAATVASTGAITGLAAADTAQQQALKAQAKAAA
                     AQQTVAEVVDRTYRTVVSESVTTAAATGGSLSPSPKPKTSASRTTTVAKPVAAPKPVA
                     APKPVVAPKPAAPKPAPKPAPVVSSGS"
     gene            13083..14108
                     /locus_tag="PLK64_00960"
     CDS             13083..14108
                     /locus_tag="PLK64_00960"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPV78338.1"
                     /translation="MTLALRAATPQPPAESRVRRGAGGTAYDVPTDPGSRSWRALGTY
                     VHLGVADATVADEAAALAAAVLSEVDVACSRFRQDSDLVRANQAGGHWVSVSPILIGA
                     VRVALRAAHSTDGLVDPTLGELMVAAGYDRTFSQLPSTVEAHALPVPVAGAWSDVRID
                     GDRVRVPHGVALDLGATGKAYAADLVALTIVERLGVAVVVSVGGDVRAHAPAGAAVTW
                     PVDLAPDLRAWSDNDPEVCRVSLTAGGLATSSVTARRWVRAGSAWHHVLDPRTGRPAQ
                     TPWLSVTAYGDCAADANTATTAALVLGADAPEWLARHGVAARLLAADGSVRRTPTWPT
                     DLEESRT"
     gene            14105..14671
                     /locus_tag="PLK64_00965"
     CDS             14105..14671
                     /locus_tag="PLK64_00965"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HPV78339.1"
                     /translation="MSLPSIDLSGPLLWFANRGTGIVLLVLYTAVVLLGILSATGRGP
                     GRFVPRFVTQGLHRNLALLATVLLAAHVTTAVVDTYVDIRWFDAFLPVGGLYRPLYLG
                     MGALSLDLLLAIGVSSLLRSRISERVWRRIHLLAYVSWGVAVIHTLGIGTDAGSPWGR
                     WTVLSCVAAVVVAVAGRAVARRVQVVRS"
     gene            14668..16026
                     /locus_tag="PLK64_00970"
     CDS             14668..16026
                     /locus_tag="PLK64_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPV78340.1"
                     /translation="MSGAVLASVTSFPGAGAGAPVAPSAPAATVRRSLSARSVPRTVR
                     TDPPAQVVATPGPVLLSGVLDGPSLEAHRDRFCQLPQLDLDTVLATVGAGAVVGRGGA
                     GFPFARKLAAAAAGRRRAVVVINLAEGEPASAKDTALALVAPHQILDGAEVVARALRT
                     TEVHIVTPLERPAVGDAIAAALSERAAQPGRRDRSQPRWRMRAAEPGFVSGQARAVLE
                     LLAGRPGLPVTAWKPEAMSGLDGRPTLLSNAETWAQVAAVLRMGALGYAAHGTDTEPG
                     TTLLTLPHRRDADTAVVLEAAYGTPWVDVLGPDVEQPVLLGGFHGTWADAGVLAEATV
                     SREELRARGLGLGAGVVLPMRADQCPVAVTSEIVDYLAKASARRCGPCLNGLPALAEE
                     MRLVALGETSASWTRIGELVGLVDKRGACAHPDGTARLVRSLLTTFDDEVALHLRGGC
                     SR"
     gene            complement(16310..17524)
                     /gene="rocD"
                     /locus_tag="PLK64_00975"
     CDS             complement(16310..17524)
                     /gene="rocD"
                     /locus_tag="PLK64_00975"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HPV78341.1"
                     /translation="MTNAPSSSADFIELEHRFAAHNYSPLDVVIARGEGAWVEDVEGR
                     RYFDALAGYSALNFGHSNPRLIEVAERQLRTLTLTSRAFHSDQLGPFARDLCALTGKD
                     LILPMNSGAEAVETAIKVTRRWGKRTKGLPEDWGTIIVMGGNFHGRTTTIVSFSTDKV
                     AREDYGPYTPGFLVVPYGDIDALRAAMTDETIGVLLEPIQGEGGVVIPPEGFLREVRA
                     LCTERNVLMVADEIQSGLARTGRTFACDHEDVVPDIYILGKALGGGIVPLSAIAANRD
                     VLSVITPGSHGSTFGGNPFAAAIGREVIAMLQTGEFQERARLLGDLLAGELAALNHPG
                     IVEVRLRGLWAGIDVDPALMSGKELCHRMADRGVLAKDTHGSTIRLAPPLVATEADIR
                     MLVSVIADALNA"
     gene            complement(17604..18488)
                     /locus_tag="PLK64_00980"
     CDS             complement(17604..18488)
                     /locus_tag="PLK64_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78342.1"
                     /translation="MGTPRAVLVRRTTELDELVAHHGTRGQAEFFLRTREQSIEPLVQ
                     RHQGTQAARQTVLAAVPADWRRAEVERAELARFVFEPDDVVIVVGQDGLVANVAKYLG
                     AQPVIGIDPLPGANPGVLVPHPAAATGDLVRDVVAGRAAYLERTMVRAVADDGQELTA
                     LNELYLGQVGHQSARYLLGWRGTAERQSSSGVIVGTGTGATGWCASIQRASAPGLALP
                     RPNDPSLVWFVREAWPSTVTGTTLAAGPLDSDELTLRVESESLVTFGDGIEADRLVLG
                     WGQVVRVGKAPRVLRTVA"
     gene            complement(18492..19505)
                     /locus_tag="PLK64_00985"
     CDS             complement(18492..19505)
                     /locus_tag="PLK64_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPV78343.1"
                     /translation="MATISTYPFVTHLRSTATMHVLHRSRGRLRHTGPGAAFWFRPMS
                     AAISEVPVDDREQPVLITVRTADLQQVSAPGTVTYRFVDPALAATHVDFSVQLKTGAW
                     AETPLETVGSAIHGATAAAVTQALVGSDLRTALTTDPAWLAATVRAALAADERLTSTG
                     LAVVGVRFAVLRAEPDVERALQTPAREAIQQEADKATFERRAVAVEREAAIGQNELAN
                     QIDLAARAEQLIAQKGTNARREAQEKAAAGAIATAAEAARTTQLAQAQAEADQVVGAA
                     AAETERAKLAAYTGMPRDLLLALAAREAAASLPQIDQLVITPDLLSSLLGRLAGGTTA
                     QEG"
     gene            complement(19575..19772)
                     /locus_tag="PLK64_00990"
     CDS             complement(19575..19772)
                     /locus_tag="PLK64_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78344.1"
                     /translation="MSRAIVAAAVLAAVAWSFWGWSWQAVVLGAYLPTLLLGLGIEYL
                     VARRRRFGNDTASPGPSKKSD"
     gene            complement(19782..20357)
                     /locus_tag="PLK64_00995"
     CDS             complement(19782..20357)
                     /locus_tag="PLK64_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPV78345.1"
                     /translation="MPYPDIAFCRLCGSPSDRRVPPGEDRERSVCRACGYVDYVNPTN
                     VVGTVPTWGPDGDHVLLCLRAIEPRKGFWTLPAGFLEYGETLADGAARETREEAGALI
                     ELDGLFTVLDVVQAGQVHVFYRARMLRPDLDPGPETTEARLVPLDEIPWDDLAFETVR
                     WTLERYVEDRSAGNFGIHTASIGRRPRESRS"
     gene            20466..21410
                     /locus_tag="PLK64_01000"
     CDS             20466..21410
                     /locus_tag="PLK64_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HPV78346.1"
                     /translation="MTTDEHTTASFEGRPLPRPDEAPFDQGLAFDLTTLLNRRSALTL
                     FGLGAAGTALAACSSGTSTGSTTSAAATTSAKSSSSSSSSSSSSSNGTSALTEIPDET
                     AGPYPGDGSNGPDVLAQSGVVRKDITASFGTSTTKAEGVPMTLELTILDIAGGGGAMK
                     GAAVYAWHCDRAGQYSMYSSGITNENYLRGVQITDANGVVSFTSIFPGCYSGRWPHIH
                     FEVYPDQASISDSTKAIATSQVALPKTACDAVYATTGYESSVGNLAKLSLTSDNVFGN
                     DGGVHQLGASTGDAASGYAVRLTVPVDTRTAPTGGSRP"
     gene            complement(21522..22490)
                     /locus_tag="PLK64_01005"
     CDS             complement(21522..22490)
                     /locus_tag="PLK64_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HPV78347.1"
                     /translation="MATAQQVCRVAYLNLRDAQRVMSLEDEQALRREAMAWLTVRTND
                     GRDAISREDLTQFRFRGERLTLIDQGRGIRKPAACRAALSIMTVHTPPGRERPYNDMR
                     GPDGLPRYKMRADSRSGADNRAVLQALELHLPVIWLVGVAPGWFQVVFPVFVIGAEPE
                     LDQFVLAYDAAQSLPLLGSPMEEVLRHYVTRETRQRVHQPVFRSMVMLAYQTQCAVCS
                     LRHSQLLDAAHIIPDSDEGGIAAVRNGLALCKIHHAAYDAGILGISPDYHVAIRDDVL
                     AEVDGPLFEHGIKGLHGQPLMVTPRRRADRPDPELLQQRYADFLSA"
     gene            complement(22577..23524)
                     /locus_tag="PLK64_01010"
     CDS             complement(22577..23524)
                     /locus_tag="PLK64_01010"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPV78348.1"
                     /translation="MHTLLPQLRPRLLRAWRAPMVVLALLFGALAMPSAAASPASAAP
                     TFGSMDAAGAVIAPLAYGGVVPATITFDGSASYCVEFCTLTSYEWDFGDGGTGTGAIA
                     SHTYTTSGYFTVTLTVRSNNGLSATASTIANVFQYTIGKFTQSTSVGLMPLSVAFDAS
                     GSLSNWDRPIVTYAWNLGDGTTGSGRTITHVFTTPGVRQVTLTVTDRLGGYQIVGGYV
                     FVQDPLLASTNLTATSPSKGVTSLTWTNRTVMISTLQIERCTGSRCTNFVPVSSVPGT
                     FTSFSESGLRSSTVFRYRIRATDYLGNTAVSSIVSVKTR"
     gene            complement(23646..24587)
                     /locus_tag="PLK64_01015"
     CDS             complement(23646..24587)
                     /locus_tag="PLK64_01015"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPV78349.1"
                     /translation="MRSLLPALRPRPLRARRVPMLLVALLFGALVMPSAAASPVPAAG
                     TSLDTVKAIIAPGTYGGQIPATLTFDGSASYCVETCSIASLSWNFGDGTTGTGTVVSH
                     TYTTSGIFTVTLTAVSNHGVTDTVTTSVMTVQQTIASFSMTPTAGVLPVTASFDASSS
                     SSNWDRPIVSYAWDFGDGTTGTGRLTTHTYTTPGVRYITLTVTDSMGTIKSTSGAITV
                     QDPLLAPNNLRATSPAKGITSLTWTNRTVMVTNLTVERCTGSTCTNFVAVGSVSGAYT
                     SFGESGLRSGTTVRYRVRATSYLGTSVVSSSVSVKVR"
     gene            complement(24811..25176)
                     /locus_tag="PLK64_01020"
     CDS             complement(24811..25176)
                     /locus_tag="PLK64_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HPV78350.1"
                     /translation="MGDEQAAYLLEDLQRECRDFRDERDWAQFHDPKSLVLALVGEVG
                     ELAELFQWVPSQEAAVRFGEGRPKERAGEEMSDIFIYLLSLAEVLDIDLLSAARAKLA
                     HARLRFPAAEVVSVAPEKA"
     gene            25464..27185
                     /locus_tag="PLK64_01025"
     CDS             25464..27185
                     /locus_tag="PLK64_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HPV78351.1"
                     /translation="MADLIDADRGGVELLVEYQLPLTSKRADVVLAGVGATTGAAAYL
                     VVELKQWTEAARFEDNDVLVSVPGYGRPVLHPLDQVRGYCEYLQDYNAALADAPDSLA
                     GIAYLHNATDLGVTDLLVRRRPGVRLFTGQRRSEFRSFLRSYFAPASGAAAADTLLAS
                     RVAPSKQLMALAAHEVQRREMFVLLDEQREAFECVRHAVGSARRANHKTAVVVSGGPG
                     SGKSVIALSLMGELAREGRTVVHATGSQSFTQTLRKVAAKGSPRVRKAFQYFNSFMES
                     EPNDLDVLILDEAHRIRETSANRYTRAAQRTGRAQVDELLSAARTPVFLLDQNQVVRP
                     GEMGTVADITAAAAARDIDVIHIQLDDQFRCGGSAVYVQWVERLLGLVPGGTLPWVGD
                     DGFRVRVMESPSEIDAWLQARAAEGYGARMTAGYCWPWSDPNRDGTLVPDVTIGDWSR
                     PWNLKGDRAVGGAPPSPLWATDPAGMGQVGCVYTAQGFEYDFAGVLLGPDLVWRDGDW
                     VAVRSGNKDPALRSRTAVSDDHFDALVRNIYKVLLTRGMQGVGIHSVDPETNELLASL
                     VRQSIKG"
     gene            complement(27175..29898)
                     /locus_tag="PLK64_01030"
     CDS             complement(27175..29898)
                     /locus_tag="PLK64_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HPV78352.1"
                     /translation="MKRRATWGWRDYPSLFWLVAAGVVALIHWMVPHAGWLMAHLVLL
                     GALTHAAMVWSTHFAQALLKTRPGLDERRTQSQRLALLFVGVSLVLVGVAAEIWWSAL
                     AGAVLVSVAVIWHGVMLWRRLRASIAPRFRISVYYYLAAAAWVPVGATLGVLLARGPE
                     DEWHGRLLVAHTMVMSLGWIGLTVTGTLVTLWPTMLRARMDPRAERFAQQALPGFAVA
                     LIVILVGATAGLRVVTVVGLVGYLLAGAWWGRALIAPLRARPPGELAPLSVAAALTWA
                     VVAIGWVAGTVALRADWTEIGDTYGVPTAVLASAFAPQLLVGAMSYLVPAVLGGGPAA
                     VRAASAWVDKWALVRLVVINGGVIVSLLPVPSIVRVLTTALVLTAYGMFVPLLGLAIR
                     ASIRARKAAESRGRDVPVPRTDLKRRSVWSSGQFVGGASALAFVVCLGVLADPAAAGL
                     TVAAGEFGAGASAGAAPVAATGHTTTVRVTAMDMRFDPPAVTVPAGDRLVIELINADP
                     TTSHDLVLANGLGTPRLRPGASTTLDVGVVGGSMDGWCSVVGHRQMGMRFAVTVVGVA
                     TPAASAAATTGAASGHGQSPGQAPRQSPGQSPGQSPGSGVDLHAPFPADFVAVPPLAP
                     APAASTAASTTHRITLTISEIDLQVAPGVWQKRWTFNGVVPGPTLRGKVGDTFEVTLV
                     NDGTMGHSIDFHAGALAPDKPMRTIPTGASLVYRFTATRSGIWMYHCSTMPMSAHIAA
                     GLHGAVIIDPPGLAPVDREYVLVQSEVYLGPNHAQGSASEVEMTRIDAETPDAVVFNG
                     VANQYDARPLTARVGERVRIWVLDAGPNRPTSFHIVGGQFDTVYAEGAWLLRAAPGGP
                     DAPGGSQIIDLAPGQGGFVELTFPEAGHYPVVSHVMVDAERGAHGVIEVSP"
     gene            complement(29895..>30703)
                     /locus_tag="PLK64_01035"
     CDS             complement(29895..>30703)
                     /locus_tag="PLK64_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78353.1"
                     /translation="LLLIVGCGLAMAVLVHLWRRQEDDPTATQILGAAMALGAAILWT
                     RLDVVDLLPWLVGYIALTIAAERVELARLTMPPHAGRLLLAIGAAVLMTAALTLLWPS
                     VGTRAFGLLTLGLVAWLARSDVARRTIRSTGLPRFSAAALLCGYVWLAVAAVVWVVGG
                     MPRALAAYDTVVHATFLGFAMSMVMAHAPVIFPSVLRRPLPYRAAMWNPLVLLQIGLI
                     VRIGPGDGLGLEGPWQIGAVLNVVALLAFAGTAVWSARSGQRARSAGGRS"
BASE COUNT         4604 a        10925 c        10530 g         4544 t
ORIGIN      
        1 caccgaccgg gtcaccgtct cggtgctcga tgtcatcagc ggcagcggct acctcatgat
       61 ccacggcaat ggggtcgcca tcccggggct gccggccgtc tccagcgggc tgggcatcgc
      121 caccggcttc cacatctcgg ccgtcctcat gggcagcaag gcgatcgggc tctatctcga
      181 ggtcgccgcg gggttcgacg cgatcgtcgg tttcgacccg ttcttcatcg ccggcaagat
      241 ctatgcccgc ggcgagctgc gcctgttcat catctcgatc ggcgcgtcgg ccgagctcac
      301 cgtcatggtg ggcaagcggg tcgtgggtgg tgtcgtggag gaccagccct acgtccacgg
      361 caaggtttgc ggctcgatcg acctgttctt cttcgagatc aagggctgtg tctcactcac
      421 catcgggtct gagcccgacg acgacccggc cgcccggccg ttggtggccg gggtcagcct
      481 cgtctcgcgc agccccgccc tcgtcgaggg gtccggcacc ggtcgggcca tcgacggcaa
      541 actgggcgac gccctcgaca cgaccgcccc ggccgcgcca ctgatcaccg tgccgctgga
      601 cgccttcccg gtcatcacct tcgacgctgc acccaccgtg ggcccgaacg ccgtcatggg
      661 ggcaaacgcg ttggggcaga ccggcgccgg ggccaacccg tggacccgca tcggcgagcg
      721 ctggtggcgt tatgaggtgc tcgacgtcca gctcaccggc gcgctcatcc ccgccaccgg
      781 gggcaagacc ccgagctcct ggtggaccgg acgccctccg gggcagcccg tcccgacgac
      841 cgcgctagcc ctgctcaact ggctgccaac ccccttctcc cgcgccatcc cctacggcga
      901 agaactcatc cgctcggtcc gggaccgctg gggcaccgtc tgccacccgg ccgcaccgtc
      961 ggcccccctg ctgtggacct tcgcggactc gccctgggga cccaatcccc ccgggtggct
     1021 cctggacggc atcgcttggc ccgacccccc cgagacggtg cgatcggcac cggcccaagg
     1081 ccggctccac gtcaccgaac cctggcggat tcctgagcgc cccgacctcg acgtgctcca
     1141 gggcaccgag cccgcgcggg tcatcggcga ccacgtgccc tgcacgaagc gcggcggcga
     1201 cacaccgacc cgcgcggcca tgacccggat gaccacctcc ctggccaggg atctcggcac
     1261 cggcccgagc gcggggtcct cggcgttcgg cgaggcggca tacgggcagg ccatggaggc
     1321 gttggccacc ggaacgtccc tcggagacct ggccgcccac cacgccacga cggcatgggc
     1381 ccccgagacc accggcgcgc agtgtcgcgg tgcgatcctg cgctcgcccg tcgacgacgt
     1441 ccccgagccg gccccccacg ggtcgccgct ggaccgcgac atcgtcaaga gaggttgggc
     1501 cgacagcgga ttcaaacccg atgacctgcg cgacgccgtc cgcctcaccc cctccgagcc
     1561 agccctcgag ttgtcggcgc ttctgctcgt tcccacccga gccctcgaag gcggcatcat
     1621 cctgcgcgtc gaggacccgg cgggcaacga gattctccag cgccccctca ccggcgccga
     1681 tgtcgtcggc accggcaacc ctctgccccc cgcgctcacc gaccccaacg gtccctggtt
     1741 cgacccgacc ctgcgagcgg cccggatcgg ctcgcgcatc ctcgccgacg acaagtccta
     1801 tgtcgccgtg tgggtgtcgc tgcgcaaact cgacgccgag atcggctcgg tcgtcatcgg
     1861 ctggcgtgag ggtgcattcg gcggcaagga ggaacggccg ttctacgtcg tcgcggtcac
     1921 cgcagtcctc ggcagcgagg cctggcggga gtcgtgggac acgacgacca tcaccgagga
     1981 ccgcgacgcc ctgcagagct ccctcaccca ggaccccgac gaccacgccc tgctgagccc
     2041 cggaacgacc tacacggtcg ccgtgacctg gcgggccgcc tcggtcaaac aggactccca
     2101 gccgacagca accaccacgc cggactggca ggccaacgag acccagtcct accggttcgc
     2161 caccgagcca gccagcgcgt cgccgaccga cctcgggccg tgggtgctcg ccagcgcgcc
     2221 tgggatgggc gacgtcggag tgttctgcac tgaaccggtg cggatcgccc tggcccacca
     2281 gcaggtcgcc gacctgttcg gggcctacgg caaggagttg cgtgtcgtcg tgcgctcggc
     2341 ctcgggcaaa cacccggcgc cacccgacgg cgggccgccc ggcgccgcgt ggaccctgcc
     2401 tctcgggcct agcgagttcc tcgctccggc cttggctctc aagatcgcga ccccgtggag
     2461 cgaggccgtg gcgacgatgc tccagacgtc cgacctcccg tgcatcgaca tctcggggac
     2521 caaggaccac acggtgacga tcaccttgcc ctacaccttc gagccgctca ccgactacct
     2581 catcgacgtc catgccgtgc cggtcggcgc cccagcgtcg gccaccggcc tggtccaccg
     2641 catcccgttc acgacctcac ggttcgagtc gctggagcac ttcgtcagct acatcgcacc
     2701 ggcctcggtg cgagcccggt tggtgcccag cccggcggcc ctcgccgccc tgcccggaca
     2761 gccgaccggc gatcaggtcg acgcggcata ccaaggtgct gggcttggtg tcccgacgac
     2821 gccgtcgttc cccgcggtcg aggtgttgtg gagcggcgac cccgtcccgc agcccgtggc
     2881 gatcatcgtg gagagcagcg aaccactctg gcgctcgcgg gtcatgcccc agcaggtcac
     2941 cggtccggtc gacgccgccg acccactgca ccactggtgg gccggggtca cgggcgactg
     3001 gctgtcgctg gctcccagca ccgcgccggt cgccgcaggt gacctgccac gagccgggat
     3061 cacccggatc gtcgcctgcc ccggacgcac ccgagccgtc gtcctgctcg gcgccggcgc
     3121 gcgaggcgcc gaggcccgac tggacctcgt gctcgccgac gacgacctcg cgggccttcc
     3181 cgaacgacgg agcaccgtcg tccgggtctc actgcagcga gccccctggg aggtggagga
     3241 ctgatgacca gggtgagcgc cttctcgctg ttccgcgcgg aagcaactgg gttgccgtgg
     3301 gcccagatcg gagtgcgcgc tctgccggtc gggcagcacg cgcccgacgg ctggttagac
     3361 gaactcaaga agcgcaccgc cgaacgcaac aacgacgtcc acgtgtggtg ggcgtgctcg
     3421 cccaccctcg gggtcccgcg cgggccgttc accgtctggg tcggtagccc ctcgctcaag
     3481 caggtgaagg atgtcgactt cacctcgtcc caacgcggcg gcgggctgct gctgcgctgg
     3541 ggaggggacg aggcggcctt catcgtcgtc gacttcgacc cgatcaaccc caacgccgcc
     3601 gtcggtctct tcgcccaccg caccgctgcg gcgatggccg acgtcgtcgc ggtgagcaca
     3661 gcgcccgcgc ctggacccgt gcacgtccaa ctcgtcatca agtgctccgg ggccacttcg
     3721 gcgctgctcg tcaacggcat gaacgccaag gtccggatcc gcaccgtcga ggacgtcatc
     3781 aacgacgaca cctggaagcc gatcgagctc gtcggcctgc cgtgcgagca accctggggc
     3841 gcaacggcat acgacagctc ggatcagggg ctcgtcgccg cccccgtgcc tcccttcgag
     3901 gcggcggtcc agcggctcaa gcggggtatg ccgccccnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnccc gtgcctccct tcgaggcggc ggtccagcgg ctcaagcggg
     4081 gtatgccgcc cctcggctgg ccaccgatga cccagaccgg ccgcctcgcc ccggactggg
     4141 tcgaccccga ccccgagctc gtcgtcaagg aggttgtcca gtcgctgctg ccggagatcg
     4201 ccgagctcta tgacccgggc gtgccagagc gcctgcagtt cacgctctcg agcgaccgag
     4261 cggtcccggg gccgacgcag gacgggcgca cgtctgccct cgacacctcg gcgacgaccc
     4321 agccgttggc aatgctcgcc ctgcctgccc agaccgaccc ggtgctcaac ttggcgaccg
     4381 gtttcggcac gacctacacc cgagagggta ggcaggacgg ggccgtcgtc ggccatcagg
     4441 acttcctcgt caccgccgac tacgaacgca ccccgagccc gtggcccgac aaggtgacgt
     4501 tcgcggcata cgccccgcag accgggccgc acgagatcgt gccgcccccg acggcgctca
     4561 ccgcagagcg caacggcctc gtgccgccca accccgcgga caccccatgg cgcgagagca
     4621 tccgggtgtc gtggaaccgc cttccgacga ccgccgcgat ggggcgggtc ggcgccggaa
     4681 cgctggctcg ctacgacgcc ggggccgccc aggccacctc cctcgccccc aagcgcgacg
     4741 ccgacggcat ccgcccgctg accctcagcc ccgatggcgc gccggggact cccggctccg
     4801 ggcgggtcgc cttcgtcgac ggcgccgcgg acatcccgat cctcagcggc gggcgccagg
     4861 tggggtatgc cgtgtcgctc gtcgacgtcc acggcgtctg gtcggcgtgg cgagacatcg
     4921 cctacaccgg caacgagccg ggaccgttgc cccctcggct catcgccctc gacctggcct
     4981 ccacgtatgc cggtagcccc gcttgcccgg cgaccctcac cctggagacg gcactcgact
     5041 ggaccgaacg ccgacccacg agcctgcaga tcgtcgccct gttcttcccc atggcctcgg
     5101 cgagcacccc gccaccggcg gggctcgacc ccggcctgcc gaccccggcc gggtgcttcc
     5161 ggcgcgatct cggcctcacc ttcgcgggcg acgtgccgac gggggtcggt gccaccgtca
     5221 cctcgctcgg cccggacgga gcaaacccca tggcgcccgg ccccggccag ggcgacggcg
     5281 ggcggcgcta ccgactgacc agcggcgtgc cgacccttga cttcaccagc acccagcggt
     5341 ggggggtcca ggtctggctg cgcagcacgt tggccgtggg agccagcccc acggcataca
     5401 cccctgacgc ggcgcacccc gcagtgacga cggccgcgag ccccgttccg atcgtgccgc
     5461 tcccgccgcc ggctccgccg ggcgtcccga tgggcagcac ccctgacgca caagggagtt
     5521 cgcatgtgcg cgtgcagtgg tcactgcccg ccggcggcac cggcgtgcgc agctgcatcg
     5581 tctgggagtg ctccgagacg gcgctgcgcc agcgcgccgg acttccgccg cgagcgcccg
     5641 agaccgactc gccgggcgtg cgcctagcgg cgttgtggac tgcctacgac gcgctgaccc
     5701 cgacccagca acgggctgcg ttccgccggt tgctcacgct gccgggcggc gcacgcgatg
     5761 ccgatgtcac cctgcccaag gggtcgaccg acatccacct cttcaccgtc accacccagt
     5821 ccgagaccgg catcgagtca ccctggcccg ggccggcgcc caccggccac atccacctgc
     5881 aggcggccac ggcccctcgg ttgcgtcggc cgacgccgcc gctgacccgg gtgagcgtgg
     5941 cgcccgacgg caccgtcacc gtgcggctct tctcggccag ccgggtgccg gtcgcccgct
     6001 tcctcgtcta tgccacacgc tccgaggccg ccgcccgcga ccacgaaagc atggggccac
     6061 cggtcgccgt cgtgcccgtc gtgctcccgc ctggcgggcc ggacccggtg accggggcac
     6121 cggtctacga agcgacctgg acggggacgc tgccgcccgc gtgggacaac tggctcgtgc
     6181 gcgccgtcgc caaaccggtc gacaccgtgc cggtccaggc ggtccgcggc cagccgtcgg
     6241 cggcctccga ggtcgtctcg atcctcgtcc cgccgaccgg gcctcccgac ctcgaccccc
     6301 tcgtcgcctc tcgctggggg gccgatcacc gtggagtcgt cgtcacgtcc ggcacctcgg
     6361 gagtggcccg ggtgaccagc gcaggcgtct tccgcctcgg cggggtcgcc gaggcgatgc
     6421 cggtggcaat gaccgacctg ccgctcctgc cggagactcc cggcaccagc gcacctgccg
     6481 gtgcgacgag ttcccctgtc ctggagcgcg gtccacgcac agcgggtcgg ctgccactgc
     6541 ggctgtggat tcgcaccccc gacgccaccg ccccggcgac ggtctcgatg cggctgctcg
     6601 acccgatggg tcgctcggtc gagcggacgg tgacggtgcc cggattcgtg ccgacaccgg
     6661 catacacgct gacgatcgtg cgcacctcga cggtggcgtc cggcgtcgtc ctcgccgtcg
     6721 gcaccgacgc gccggcaacc gtgagcgcgg ggatcagcct gtccgtccgc ggggtgtcga
     6781 cggacctcac ccccctcccg ctgccgttcc ccagaccgag gccaggactc ctgcgagtgt
     6841 cggggacctt cctgctctcg ggggtgcgca cggggcggat caccttccct gcggacggcc
     6901 agatccatgt catccgtccg cagcggaccc tggggtggcc gcgggaggcg aggtatgccg
     6961 tgttcgtccc gctcgtcagt cccgtccgga tcaccctgat gctgctgggc ccggcgggcg
     7021 agcagttggt gaccgtcacg ggcggtgcct gagcgggcgg caccgaccgt cgtcgcggcc
     7081 cagacacgtc ggtcaggagg cgaactgaca ggatgtcggg catgacgacc ccgacgaagg
     7141 cgccttcgat cgtgctttcg ccacggctcg ggggcggtcg caccctcgaa tcagtggcga
     7201 ggcgctgcct tgagcacacc gacgccaacg aaccactcct ggacgcccat ggcgatcacg
     7261 aagcgctaca ccagttccgg gttggctatc ggcggctgcg gtcgctgttc tccctcgcgc
     7321 gcgaggtcag ccgggccgat cccgaagcac ggcggctcaa ggcgcagctg cgtgagctca
     7381 ccgtcgcgtt cggcccggct cgcgacctcg acgtcttcct cgaagggcac ccggacctca
     7441 gccccaccaa cgcagcgcgc ctggaagacg caagggttac ggcatacagc gaggcactgg
     7501 agttcctgca ctcgcccgag tgggcgcaga cccgagtcga cctcgacgag tggctgcgga
     7561 acgggtccta ccgcgacctg ctcggcactg gcacgtggag cggccgagcg tggacggccg
     7621 gcgccctcga acgacgccgc aagcggatcc gcacgatgag caggcgactg gccgacctct
     7681 ccgaccacga tcgccaccag ctgcggatcg aggcgaagaa ggttcgctac ggctgccagt
     7741 tcttcgggac gatgtggccg cggactgcct ccgatgccct cgtcatggag aagaccctcg
     7801 gtgccctgca ggatcgtctc ggcgcgctca acgatctcgc gacgtggagc cgactggtcg
     7861 acctcacggg ggtgactgat ctgctgccgc ccgaggtgga cgtcgaggcc gagttggagg
     7921 ccgcgcagga gttggcggat cggatcgcgc aactgcccgg cttctgggag gccacgcttc
     7981 agaagtgagg ggcgccagac gtgaagggcg ttgcagagta tggcgagtga tgggccgatg
     8041 gccggacccc tgacaccagg aggggtccgg ccatcgttcg cgccgcggtc ccgcatcagc
     8101 cgacagcgcg gtggatgtct ttcgggggtc cgatttgggc cggtcccgat caacgtcact
     8161 gcttcccaac agtgcttgaa ttctcaccgg cccggacacg tcggacaagg ctggttcgat
     8221 atggattgtc gggtatgtga acgcgcccgc ttgtgaacgc ccgctccccg tgccacgatc
     8281 gccctgggtg tgaacgttca cggaaggact gtgaatctct ggtggtgatc gacaccggat
     8341 cgaaccgctt ctcgggggag ttgcgggtgg cgctccacgc gagcggcctc accttggatg
     8401 ccgtccaacg tcgtttggcc gagcgcggtt tcgcgatcgg tcgcagcacc ctgagctact
     8461 ggcagaacgg tcaccgcctg cccacccagc cgacctcgct cctcgccgtg accgccctcg
     8521 aggaaatcct caaggtgcca atcggcagtt tcaccgaggc cttgcgccac ccgaactcga
     8581 ccccggtcag cccagagctc gacatggccg ccgcggccgc ccgaatcggc gtcctcatgg
     8641 agggcgtcgg ctgccccgac ggcatggcca ccctcgaacc gttgtccgcc atccaggtcg
     8701 ccgagttcgg acccacgggg tgcctgcggt cggtgcgcgc ggtcgagacc tatcgcgccc
     8761 tggtcgacac cgatcggttc ccgatcctgc acggcgggga accgggcggg cgacctgagc
     8821 tcatccgcca cgaggcggtc agcggcggac gcatcgggcg ggtccgccgg gatgcggagg
     8881 ccaacgtcgt cgtcagcgaa ctcgtcttcg atcgcttcgt ccgccgcggt gagcaccatt
     8941 tcctgcacta cctcacgttc gatgacaacg agcagcgcag cctcctgctc tatcggctcg
     9001 tcgtcacctc gcgctcgatg atggtcctcg acctgggctt ccaccccgac tgtctgccgg
     9061 tgcgcctcga ggagttcgag cgggccaccg acaacggccc cgacctgttc gtccgccaac
     9121 ggacgctctc accggaccgt cgagtcaccc tggtgcgcga gcgcgcgcgc cgcgggatcg
     9181 tcggcctccg ctgggaatac gcctagcccc cacggggtat gccgcccgct cacctcaagc
     9241 ggccgcggct cgctcaatcg gtggtgaaaa ccgggagacc cgcctcacag cgaaccttca
     9301 cctcgacctc ttcctcctcc gctcccgcga gttggaactt cacctcgaag cgcccgttgt
     9361 cttcgcgcga gaactcgaag ctccagccgt cggtcgccac gacgctgcgg cggtacgcgg
     9421 tgtcgccgac gcagtaggcc cagacctgac cggccgccgt gctgactgac cggaccaccg
     9481 gacggggcga ctcggtcggc tcgggcttcg gggtgcttgg ggagctcggg gaggtcgacg
     9541 actgcccgct ggaggtcggt gccgacgtca ccggcggcgg gggcgggacc gactgggtcg
     9601 cgctgggcgt caagccggcg gtcgtcgggg ccgccgccga gccgagtggg accggcgcgc
     9661 cgaggagtgt ggccgcctct ccggcccgag agatcgcgcc ccacgacagc ccagccgtca
     9721 cgctgaccac gagcacccag gttgcggcat accgcagcag ttgtgcccgc gatccgctca
     9781 tcggttcctc cccatgctgc gcgtagcgta acccccatgg ctcgcgtgct cgtcgtcgaa
     9841 gatgatcccg cgatccgtgg cgcgttgttg cgcagtctgg ccggcgcggg ccacgcggtg
     9901 accagccagc cgaccggcat ggccgggctg accgaggccg tcgaccagcc tccggatgtc
     9961 gtcgtgctcg acctcgggtt gccggacgtc gacggcctga ccgtgctgtc gatgttgcgc
    10021 gccgtgagct cggtgcccgt ggtcgtcgca acggctcgcg acgacgagaa ggagatcgtt
    10081 cgagccctcg acctcggcgc cgacgactac ctcgtcaagc cgtttggcgg tgaccaactc
    10141 gaggcacgca tccgtgcggt cctgcgccgg gcggggcacg acgcgccgcc gacacccatc
    10201 gtcgtcggcg gtctggtcat cacctcggcc agtcgcgagg tgacggtgga cgggataccc
    10261 gtggagttgt cccgcaagga gttcgacctg ctgcacctgc tggcccagcg ggtcggcgag
    10321 gtcgtcacca agcgcgagat cctcaccgag gtgtggcggc agagttatgg cggcggcgac
    10381 cggacggtcg atgtccacct gtcctggctg cggcgcaagc tcggcgagac cgccgccgat
    10441 ccggtctatc tccatagcgt tcggggggtc ggtgtgaggt tggttgaccc gcgcggggcc
    10501 caccccgggt cggctgactg atgctgaggt ctcggatcac gtggctggcc gcagcgttgg
    10561 tcgccggcgt cgtgatctcg ttcgtcatcc ccctgtgcct gctcgtgcgg accatggccg
    10621 aggagcgagc ggtctccgcg accaacagtg acgcccagag cgtggcgatg atcgtcgccg
    10681 gggtcgacga cccggcgtcg ctcccggcag tcctggcccc caccctcggt gcccgcacgg
    10741 cgacgacgac cgtcgctctc ccctccggtc aacttattgg aggcccgaca accaccggcc
    10801 ctgtcaccac cggtcagccc tcgtccggcc cggcgcagtt cccggaggat gcccgccttc
    10861 tcgccgcggt ccgggccgac aaccggtcga tggcgacgac cgtcgatgac cgcttccacg
    10921 tgctgatccc cgtcggtgtc gccgacgggg tcgcgatcgt gcactcggtc accgacctcg
    10981 gacgggtgcg gcgcggggtt tacggctcgt gggcgatcct cgtcgcggtc ggcctgttgc
    11041 tcgtcgtggt gagcgtgctc gtcgcccggc gtcagagcgc ccggatcagc acaccggtcg
    11101 tcgcgatggc tggtgtcgcc caccggctgc gatccggcga cttcgaagcc cgagcagcgg
    11161 ccacggggcc ggtcgaggtc gtcgagcttg gacatgccct caatcaactc gcggaccgga
    11221 tcgaggacct gctgcggatc gagcgcgagg gcgccgccga cctgtcccac cggctgcgca
    11281 ctccggtcac ggcactgcgg ctggacaccg atcaggtgga ggacccgacg gttcgggccc
    11341 ggttgcgcag ccatgtcgag gacctgcacc gcgccatcga ctcggtggtc gccgacgccc
    11401 ggcgcccgag ccgcgacgca atgggcggat ggtgcgacgc ccgagtcgtc ctcgccgacc
    11461 gggcccatca ctggggtccg ctggccgagg accaggggcg catgaccacc gtgatcggcg
    11521 gctcggcgaa cggttcggcc agcggggcgg cgggagagag cgtcacgggt gagcacccgg
    11581 gaggtgagca cgcggcatac cgaccggcgt tggtcgggct ggcggctcgg gacctggccg
    11641 acctgctgga caacctcgtc gacaacgcgt tcgcgcacac gcctgaggag acgccaatcc
    11701 ggttggagtt gcacgtcgtc gatgggctgg tggtcctgcg ggtcgaggac ggcggggacg
    11761 gcagccccga cacggttgcg ttcgcgcgcg gtgagagtgg cgccgggagc accgggttgg
    11821 ggctggacat cgtccgccgc ctggcggctg acgcgggagg gtcggtcggt ttcgaacggg
    11881 ccgggcttgg cggcctcgcc gtgatcgtcg tcctgcccct catcgagcgc gccgactgaa
    11941 tcccactgcg tcgcaagctt gccccggccc ggtgcgggtt ggtcagtcgt cgtcaccgtc
    12001 gcgctcgtcg tcgtcaggct cgtcgtcacc gtcgcgctcg ttctcatact cgtcgtcgtg
    12061 gtcgtcgacc gtcgcggccg ccggcgctgc ctccggggtc ggggccggcg tgtaccgggt
    12121 cgcggacgtg gtcgctgatg acgcagccac cgccgggacg ggaacggccg ccggggcagg
    12181 cgcggccacc gtgcgggtga cgctcacgtg ggtgacgcac tcatcgccct cgagaaccgc
    12241 cggctccacg cagtcggcat agaccgcggt cgggacggtc tgcgagatcg ggatcgacgc
    12301 cgatggggcg gccgaacccg cggccagcga gctatagagc gcgacggcga tcgccccccc
    12361 actgacgact ccgaggctga tggccttcca tccgatccac atgaggttgc cttccgattc
    12421 gatgtgcgag gtgcagggac cgcgggcgcc atcgacgtcg gcgtgggaac gcggtgcacc
    12481 ttcacggtga ctcaccttgg gtcaaccgca ccctcgggca gcatcaaggg agtgctaaga
    12541 attcggccgc gcatccgccg tccaggcctc caggcgctac cgctgaccgg acttcttagc
    12601 gttgtcttga ggtgcgcctg acgcgccctt gccgcagccg cgtcgacgat gaaccacatg
    12661 aatcgaactt ggttgcccgc ctctcgcgac cggtccatga ccgccatgca cgccctcacc
    12721 ggcgtggtcg ctgccgccac cgttgcctcg accggcgcca tcaccggtct ggcggcggcg
    12781 gacaccgcgc agcagcaggc gctcaaggcc caggcaaagg cggcagccgc acagcagacg
    12841 gtggcggaag tcgtggaccg cacctaccgc accgtggtca gcgaatcggt gacgacagca
    12901 gccgccacag gcggctcgct cagcccttcg ccgaaaccga agacgagcgc gagcaggacg
    12961 accacggtgg ccaaaccggt cgcagccccg aaaccggtcg cagccccgaa gccggtcgtg
    13021 gctccgaagc cggccgcgcc caagccggcg ccgaaacccg cccccgtcgt ctcatcaggg
    13081 tcgtgaccct cgccctgcgc gccgcaactc cccagccccc cgccgaatcc cgcgtaaggc
    13141 ggggggctgg ggggacggca tacgacgtcc cgaccgaccc gggctcgcgc tcgtggcgag
    13201 cactgggaac ctacgtccac cttggcgtgg ccgacgcgac cgtcgccgac gaggctgcgg
    13261 cgcttgccgc cgcagtgctg tccgaggtcg acgtggcctg cagtcgattc cgccaggaca
    13321 gtgacctggt ccgggccaat caggctggcg gccactgggt gtcggtcagc ccgatcctca
    13381 tcggcgcggt ccgggtggcg cttcgagccg cccactcgac cgacgggctg gtcgatccca
    13441 ccctcggaga gctcatggtc gccgcgggct atgaccgcac cttcagccaa ctccccagca
    13501 ccgtcgaggc ccacgccctg cccgtgccgg tcgccggcgc ctggtcggac gtccgcatcg
    13561 acggtgaccg ggtgcgcgtc ccgcacggcg tcgccctcga cctcggcgcc accggcaagg
    13621 cgtatgccgc cgacctcgtc gccctcacca tcgtcgaacg gctcggcgtg gctgtcgtcg
    13681 tcagcgtcgg cggagacgtc cgcgcgcacg ccccggccgg cgccgcagtc acctggccgg
    13741 tcgacctcgc gcccgacctg cgcgcgtggt cggacaacga ccccgaggta tgccgcgtgt
    13801 ccctgaccgc gggcgggctg gccacctcat cagtcaccgc ccgacggtgg gttcgggccg
    13861 gatcggcctg gcaccacgtc ctggacccgc gcaccggtcg accggcgcag accccctggc
    13921 tgtcagtcac ggcatacggg gactgcgcgg ccgatgcgaa caccgcgacg accgccgcgc
    13981 tcgtcctggg ggccgacgcc cccgaatggt tggcccgaca cggcgtcgcc gcgcgcttgc
    14041 tcgcggccga cgggtcggtc cgccgcaccc ctacctggcc cacagacctt gaggagtccc
    14101 gcacgtgagc cttccgtcca tcgacctgtc cggtccgctc ctgtggttcg ccaacagggg
    14161 caccgggatc gtcctgctcg ttctctatac ggcggtcgtg ctgctcggca tcctgtcggc
    14221 aaccggtcgc gggcccggtc ggttcgtccc gaggttcgtc acccaggggc tgcaccgcaa
    14281 cctcgcgctg ttggccaccg tcctgctcgc cgcccacgtg acgacggccg tcgttgacac
    14341 ctacgtcgac atccggtggt tcgacgcatt cctgcccgta ggggggctgt atcgcccgct
    14401 ctacctcggg atgggggcgc tgagcctgga cctgctgctg gccatcggcg tgagcagcct
    14461 gctgcgctcg cggatctccg agcgggtgtg gcggcgcatc cacctgttgg cttacgtgag
    14521 ttggggtgtc gcggtgatcc acaccctcgg gatcggcacc gacgccgggt cgccgtgggg
    14581 acgctggacc gtgctgagct gtgtggcagc cgtcgtcgtt gccgtcgccg gtcgggccgt
    14641 ggcccgccgc gtccaggtgg ttcggtcatg agcggggccg tcctggcctc ggtcacctcc
    14701 ttccctggcg cgggcgccgg agcccccgtt gcaccctcgg cacctgcggc gaccgtgcgc
    14761 cggtcgctca gcgctcggtc ggtgccacgc acggtccgca cggatccgcc tgctcaggtc
    14821 gtcgccaccc cagggccggt gctcctgtcc ggcgtgctgg acggaccgtc gctggaggca
    14881 caccgcgacc ggttttgcca gctccctcag ctcgacctcg acacggtctt ggccacggtc
    14941 ggcgccggcg ccgtcgtcgg ccggggcggc gccggtttcc ccttcgcccg taagttggcc
    15001 gccgcggcgg ccgggcggcg ccgagcggtc gtggtcatca acctcgccga aggcgagccg
    15061 gcctccgcga aggacacggc gctcgccctc gtggcgcctc accagatcct cgacggggcc
    15121 gaggtggttg cgcgcgcact gcgcaccacc gaggttcaca tcgtcacgcc gctggagcga
    15181 cccgcggtcg gcgacgccat cgctgccgcc ctttccgagc gcgcggccca gcccggccgc
    15241 cgcgaccgga gccagccgcg ctggcggatg cgggccgccg aaccgggctt cgtgtctggc
    15301 caggcgcggg ccgtcttgga actcctcgcc ggtcggcccg gcctgccggt gacggcatgg
    15361 aagcccgagg ccatgtcggg tctcgacggg cgccccaccc tgctgtccaa cgccgagacc
    15421 tgggcgcagg tcgccgccgt cctgcggatg ggagccctgg ggtatgccgc ccacggcacc
    15481 gatacagagc ccggcacgac tctcctcacc ctcccgcatc ggcgcgacgc cgacaccgcg
    15541 gtggtgctgg aggcggcata cgggacgccg tgggtggacg tgctgggacc ggacgtcgag
    15601 caacccgtcc tgctgggcgg tttccacggc acgtgggccg acgcgggtgt cctcgccgag
    15661 gcgacggtca gccgcgagga actccgcgcc cgtggactcg ggttgggcgc aggggtggtc
    15721 ctgcccatgc gggcggatca gtgcccggtg gcggtcacca gtgaaatcgt cgactacctc
    15781 gccaaagcct ccgctcgacg gtgcgggccc tgcctcaacg gcctgcctgc ccttgccgag
    15841 gagatgcggc tcgtggccct gggcgaaacc agcgcttcgt ggacccggat cggagagttg
    15901 gtgggcctgg tcgacaaacg cggcgcgtgc gcccatccgg acgggacagc ccggttggtg
    15961 cgatcactgc tgaccacgtt tgatgacgag gtcgccctgc acctgcgtgg ggggtgctcg
    16021 cgatgacgtg gacgatgcgt gtcgattggc cgtcatgcgc cgcccggggc ttgtgcgccg
    16081 agctgttgcc tgagcgaatc cacttggacg agtggggttt tcccgtcgtg gaagggcccg
    16141 tgacccccga cctgctgggc ttggcgaagg ccgctgccac tgcgtgcccg catcgagccc
    16201 tgcgactggt gtcagccggg taagcaggcc tggccctccg cgcaaggtgg caaacggtgg
    16261 cctcggcgga cggcataccc actgaatccc acctttcggc gaggtccggt caggcgttga
    16321 gggcgtcggc gatcaccgag acgagcatgc gaatgtcggc ctcggtggcg accaatggcg
    16381 gcgccagacg gatcgtcgag ccgtgcgtgt ccttggcgag gacgccccgg tcagccatcc
    16441 ggtggcacaa ctccttgccg ctcatcaggg ccggatcgac gtcgatgccc gcccacagac
    16501 cgcgcaggcg aacctcgacg atgcccgggt ggttcaaggc ggcgagctcg ccggccagga
    16561 ggtcacccag gaggcgagcg cgctcctgga actcgccggt ctgcagcatc gcgatgacct
    16621 cgcggccgat cgccgcggcg aaggggttgc cgccgaaagt cgagccgtgc gagcccgggg
    16681 tgatgacgct gagcacatcc ctgttggcgg cgatggccga cagcgggacg atgccgccac
    16741 cgagcgcctt gcccaagatg tagatgtcgg gaacgacgtc ctcgtgatcg caggcaaagg
    16801 tgcgaccggt gcgcgcgaga cccgactgga tctcgtcggc gaccatgagg acgttgcgct
    16861 cggtgcacag cgcgcgcacc tcacgcagga aaccctccgg cgggatcacc acgccgccct
    16921 cgccctggat cggctcgagg aggacaccga tggtctcgtc ggtcatggcg gcgcgcagcg
    16981 cgtcgatgtc gccatagggg acgacgagga agcccggggt ataggggccg tagtcctcgc
    17041 gggcgacctt gtcggtcgag aacgagacga tcgtcgtcgt gcggccatgg aagttgccgc
    17101 ccatgacgat gatcgtgccc cagtcctccg gcaggccctt ggtccgcttg ccccaccgcc
    17161 gggtcacctt gatcgcggtc tcgacggcct cggcgccgga gttcatcggg aggatgaggt
    17221 ccttgccggt cagggcgcac aggtcgcggg cgaacggccc gagctggtcg gagtggaagg
    17281 cccggctcgt cagcgtcagg gtgcgtagtt gccgctcggc gacctcgatg agccgcgggt
    17341 tggagtgccc gaagttgagc gccgagtaac ccgccaacgc gtcgaagtag cggcgccctt
    17401 cgacgtcctc cacccaggcc ccttcgccac gggcgatgac gacgtccagc ggagagtagt
    17461 tgtgggccgc aaagcggtgc tccagctcga tgaagtcggc gctggacgag ggggcgttgg
    17521 tcatggatgg ctttctctcg tggaggacag ttccccagta tgccgcgtgc tccccgctgc
    17581 gggcgatgct ctaggccgca ggctcaggcc acggtccgca gaacccgggg ggcctttccg
    17641 acgcgcacga cctgccccca cccgaggacg agccggtccg cctcgatccc gtcaccgaag
    17701 gtgacgagcg actcggactc gacccgcagg gtgagctcgt cgctgtccag cgggcccgcc
    17761 gccagcgtcg tgcccgtcac cgttgagggc cacgcctccc ggacgaacca caccaggctc
    17821 gggtcgttgg gccgcggcag tgcgagacca ggcgccgacg cccgctggat cgaggcgcac
    17881 catccggtcg ccccagtccc cgtcccgaca atcacccccg aggacgactg gcgctccgcc
    17941 gtgccgcgcc acccgagcag gtagcgcgct gactggtgtc ccacctgccc gaggtagagc
    18001 tcgttgaggg ccgtcaactc ctgcccgtca tcggccaccg cgcgcaccat ggtccgctcg
    18061 aggtatgccg cccgccccgc gacgacgtcg cgcaccaggt cgcctgtggc cgccgcgggg
    18121 tgggggacga gcactccggg gttggcgccg gggagggggt cgatgccgat caccggctgc
    18181 gcgccaaggt atttcgccac gttggcgacc aggccgtcct gcccgacgac gatgacgacg
    18241 tcgtcgggct cgaagacgaa cctcgccaac tcggctcgct cgacctcggc gcggcgccag
    18301 tccgcgggca ccgcagcgag gaccgtctgg cgggcggcct gggtcccttg atgccgttgg
    18361 acgagcggct cgatcgactg ctcacgggtg cgcaggaaga actcggcctg gcctcgcgtc
    18421 ccgtggtggg ccaccaactc gtccaactcg gtggtccgcc ggacgaggac ggctcggggc
    18481 gtccccatgg gtcagccctc ctgggcggtg gtcccgccgg ccagccgacc cagcagcgag
    18541 gacagcaggt cgggggtgat gacgagttgg tcgatctgag gaagcgacgc ggccgcttcg
    18601 cgcgcggcga gcgcgagcag gaggtcgcgc ggcataccgg tgtatgccgc caacttcgcc
    18661 cgctcggtct ccgccgccgc tgctccgacc acctggtcgg cctcggcctg ggcctgggcg
    18721 agttgggtcg tgcgagcggc ctcggcagcc gtggcgatgg cgccggccgc cgccttctcc
    18781 tgggcttcgc gccgggcgtt ggtgcccttc tgcgcgatga gttgttcggc gcgagcggcc
    18841 aggtcgatct ggttggccaa ctcgttctga ccgatagccg cttcgcgctc gacggcgacg
    18901 gcccggcgct cgaaggtggc cttgtcggcc tcctgctgga tcgcctcgcg ggcgggggtc
    18961 tgcagggcgc gctcgacgtc gggctcggcg cgcagcacgg cgaaacggac cccgacgacc
    19021 gccagaccgg tggaggtcag ccgctcgtcg gctgcgaggg cggcccggac ggtggcggcc
    19081 aaccaggccg ggtcggtggt caaggcggtc cgcaggtcgc tacccaccag ggcctgggtg
    19141 accgctgctg cagtggcgcc gtggattgcg gatccgactg tctcgagcgg ggtttcggcc
    19201 cacgcgccgg tcttgagctg gaccgagaag tcgacgtggg tcgccgcgag ggcggggtcg
    19261 acgaagcggt aggtgaccgt gccgggggcg ctgacctgct gcaggtcggc ggtgcgcaca
    19321 gtgatgagca ccggctgctc gcggtcgtcg acggggactt cgctgatcgc cgccgacatc
    19381 ggccggaacc agaacgcggc gccggggccg gtgtgccgga gccggccgcg cgagcggtgc
    19441 aggacgtgca tggtcgccgt gctgcgcagg tgggtgacga acgggtaggt gctgatggtg
    19501 gccatgaggc tctccttgga tcagggttga cggcttttcg tcactctgac tctataggcc
    19561 ttctggcgtt attgtcaatc tgactttttt gacggtccgg gggacgcggt gtcgttgccg
    19621 aagcggcgcc gacgcgcaac gaggtactcg atccccagac caagcaggag agttggaagg
    19681 tacgcaccca ggaccaccgc ttgccatgac cagccccaga acgaccacgc cactgcggcc
    19741 agcaccgcgg ccgccactat cgcccggctc atgcgcatga cctagctcct tgactctctc
    19801 ggccgtcgtc cgatggatgc ggtgtggatg ccgaagttcc cggcggatcg gtcctcgaca
    19861 tagcgctcga gggtccagcg cactgtctcg aaggccaggt cgtcccaggg gatctcgtcg
    19921 agaggcacaa gccgcgcctc ggtcgtctcg gggcctgggt cgaggtcggg gcgcaacatt
    19981 cgagcccggt agaacacgtg gacctgacca gcctggacga catcgagcac ggtgaagagc
    20041 ccatcgagct cgatcaaggc gcccgcctcc tcgcgggttt cgcgggccgc accgtccgcg
    20101 agcgtctcgc cgtattcgag gaacccagcc ggcagggtcc agaagccctt ccgcggctcg
    20161 atggcccgca gacacaacag cacgtgatca ccatcggggc cccaggtggg cacggtgccg
    20221 accacgttcg tggggttcac gtagtcgacg tagccgcagg cacggcatac cgaccgctcc
    20281 cggtcctccc ccggcggcac gcgacggtcc gacggggagc cgcagaggcg gcagaacgcg
    20341 atgtccgggt agggcacgag cctcaatcta cgcggggcag gtgacgcgcc agggttcact
    20401 ggtgcccaca aagcctcaca ggaaactcac tgcaagcgct tagattgatc ccctagtctc
    20461 ccgccatgac gaccgacgaa cacacgaccg catccttcga aggccggccg ctgccccggc
    20521 ccgacgaggc tcccttcgac caggggttgg ccttcgacct caccacattg ctcaaccgcc
    20581 gcagcgccct gacgctcttc gggctcggcg cggccgggac cgcgttggcg gcctgcagct
    20641 cgggcacgtc caccggctcc acgacatccg ccgcggcgac cacgagcgcg aagtccagct
    20701 ccagttcgag ctcaagctcg agttccagct ccaacgggac gagtgcgctg accgagatcc
    20761 ccgacgagac ggccggtccc tacccgggcg acggctccaa cgggccggac gtgctggccc
    20821 agagcggcgt cgtccgcaag gacatcaccg cgagcttcgg cacgtcgacg acgaaggccg
    20881 agggcgtccc gatgaccctc gagctcacga tcctggacat cgccggcggc ggtggcgcga
    20941 tgaagggcgc cgccgtctat gcgtggcact gcgaccgggc cgggcagtac tcgatgtact
    21001 cctccggcat caccaacgag aactacctgc gtggcgtgca gatcaccgac gccaacggcg
    21061 tcgtctcgtt cacaagcatc ttccccggct gctactccgg gcgctggccg cacatccact
    21121 tcgaggtcta ccccgaccag gcgagcatca gcgactcgac caaggcgatc gccacctcgc
    21181 aggtggccct gcccaagacg gcgtgcgacg cggtctacgc gacgacgggc tacgagtcct
    21241 cggtcggcaa ccttgccaag ctctcgctga ccagcgacaa cgtcttcggc aacgacggcg
    21301 gcgtgcatca gctcggcgcc tcgactgggg acgccgccag tgggtatgcc gtgcgcctca
    21361 ccgttcccgt cgacacccgg accgcgccca ccggcggatc ccgcccctga gcccagccgc
    21421 gcggcatacc ctatgccgtg tttgacacct cccaacgctc cccaacgctg ggtcgaggta
    21481 atcagccatc ccccgatgat ggcgacatac ctcgacccgg cttaggcgct gaggaagtcg
    21541 gcgtagcgct gctggagcaa ctccgggtcc ggccgatcgg cccgccgtct cggtgtgacc
    21601 atcagcggtt ggccatgcag gcccttgatg ccgtgctcaa acaggggtcc gtcgacttcg
    21661 gcaagcacgt cgtcacggat cgccacgtgg tagtcggggg agatccccag gatgcctgcg
    21721 tcgtatgccg cgtggtgaat cttgcacagc gccaatccgt ttcgcacggc ggcgatcccg
    21781 ccctcgtcgg agtcggggat gatgtgcgcg gcgtcgagga gttggctgtg ccggagggag
    21841 cagacagcgc actgcgtttg gtaggccagc atgaccatcg aacggaacac cggctggtgg
    21901 acgcgctgac gggtctcgcg agtgacgtag tggcgaagca cttcctccat gggcgatccg
    21961 agcagcggca acgactgggc cgcgtcatag gccagaacga actgatccag ctccggctcg
    22021 gcgccgatga cgaagaccgg aaacacgacc tggaaccatc ccggcgccac tccaaccagc
    22081 caaatcacgg gcaggtgcag ctccaacgcc tgcagcaccg ctcggttgtc cgcgccgctg
    22141 cgcgagtcgg cccgcatctt gtaccgaggt aggccgtccg gaccacgcat gtcgttgtag
    22201 gggcgctcgc ggcccggggg cgtgtgcacc gtcatgatcg agagggccgc gcggcaggcc
    22261 gccggcttgc gtatgccgcg tccctgatcg atgagggtga gtctctcgcc gcggaacctg
    22321 aactgggtga ggtcctcccg actgatcgcg tcccgaccgt cgttcgtccg taccgtgagc
    22381 cacgccatgg cctcacgccg tagcgcctgc tcgtcctcca gcgacatcac tcgctgagca
    22441 tctcgcagat tcaggtatgc cacgcggcat acctgctgcg ctgtcgccac ctgccgggtc
    22501 ctaccagttg ccgggtcgag gtattcggcc atctgcagac gacggcggaa tacctcgacc
    22561 cggcattagc gtggtgtcag cgggtcttga ccgagacgat cgaggagacg gctgtgttgc
    22621 cgaggtagtc ggtggcccgg atccggtagc ggaagaccgt cgaggacctc agacccgact
    22681 cggagaagct cgtgaaggtc cccgggactg acgagaccgg gacgaagttc gtgcacctgg
    22741 agccggtgca tcgctcgatc tgcaacgtgc tgatcatgac cgtccggttg gtccaggtga
    22801 gcgacgtgac ccccttcgac ggggacgtgg ccgtgaggtt ggtcgacgcg agcagcgggt
    22861 cctggacaaa gacatagccg ccgacgatct gatacccgcc gaggcggtcg gtcacggtga
    22921 gggtgacctg acggacgccc ggcgttgtga agacgtgggt gatcgtgcgc cctgagccgg
    22981 tcgtcccgtc cccgagattc caggcgtagg tgacgatggg gcggtcccag ttggacagcg
    23041 acccgctggc gtcaaatgcg acgctgaggg gcatcagtcc aacgctggtc gactgggtga
    23101 acttgccgat cgtgtactgg aacacgttgg cgatcgtgct cgccgtggcc gacaacccgt
    23161 tgttggagcg cacggtcagc gtcacggtga aatagccact tgtcgtgtag gtgtggctcg
    23221 cgatcgcgcc ggttccggtc cccccatcgc cgaagtccca ctcgtaggac gtgagggtgc
    23281 agaactccac gcagtaggag gccgaaccgt cgaaggtgat ggtcgccggg acaacgccgc
    23341 catacgccag cggcgcaatc accgcgcctg cggcgtccat cgacccgaag gtcggcgccg
    23401 cggaggcggg gctggccgcc gcgctgggca tcgccaacgc gccgaagagg agggccagga
    23461 caaccatggg cgcgcgccaa gcacgcaaaa gtcggggccg cagttgggga aggagtgtgt
    23521 gcatgagcgg gagttcctct ctaaagggac gacaccaccg gctgaaccac cgccgggtcg
    23581 aggtattcgg ctatcaacag acgacggcgg aatatctcga cccggcaagc ggggagcggg
    23641 aggggtcagc gaaccttgac cgacacgctt gaggagacca ccgaggtgcc caggtagctg
    23701 gtcgcccgga cccggtaacg gaccgtcgtc ccagaccgca ggcccgactc gccgaagctc
    23761 gtgtacgccc ccgacaccga cccgactgcg acgaagttcg tgcacgtcga gccggtgcag
    23821 cgctcgaccg tcaggttggt caccatgacc gtgcggttgg tccaggtcag cgaggtgatc
    23881 cccttggccg gggacgtggc ccggaggttg ttcggcgcca gcagcgggtc ctggacggtg
    23941 atcgcgccgg acgtggactt gatggtgccc atgctgtcgg tcaccgtgag ggtgatgtag
    24001 cgcaccccgg gagtcgtgta ggtgtgagtg gtgaggcggc cggtcccggt cgtgccgtca
    24061 ccgaagtccc acgcgtagct gacgatcggg cggtcccagt tggagctgga ggagctggcg
    24121 tcgaagctcg cagtcaccgg caacaccccc gccgtcgggg tcatcgagaa gcttgcgatc
    24181 gtctgctgga cggtcatgac tgatgtcgtc acagtgtcgg tcaccccgtg gttcgacacc
    24241 gccgtcaaag tcacggtgaa gatgccgctc gtcgtgtagg tgtggctcac cacggtgccg
    24301 gtcccagtcg tgccgtcacc gaagttccac gacagcgacg caatcgagca cgtttccacg
    24361 cagtaggagg cggacccgtc gaaggtcagg gtcgccggaa tttgtccacc gtacgttccc
    24421 ggcgcgatga tcgccttcac ggtgtccagc gatgtcccag ccgcggggac cgggctggcg
    24481 gcagcgctgg gcatgactag cgcgccgaag aggagggcga caaggagcat gggcacgcgg
    24541 cgggcgcgta gtggtcgggg acgcagggcg ggaagcagtg agcgcatgag cgggaaatcc
    24601 tctcgaacag gtcgtcgccg agcgcaagag aatgccccga ccatgccgac gctagggaca
    24661 acggggttcg cgcgctattg gaccttgggg cagttgcctg tgtcctaaaa gtccggtatg
    24721 ccgccctgcc ggactggccg atagggcgcc gggacccctg gaagaaccgc gtacctcagg
    24781 cacgcactgc gtgttcgttt ggtcacaccg ctaagccttc tcgggggcaa cgctgacgac
    24841 ctcggcggcc gggaaacgca ggcgcgcatg cgccaacttg gcgcgtgcgg cagacagcag
    24901 atcgatgtcg agcacctcgg ccaacgacag caggtagatg aagatgtcgc tcatctcctc
    24961 acccgcgcgc tccttgggcc tgccttcccc gaaccgcaca gcggcctctt gcgatggcac
    25021 ccactggaac aactcagcga gctcgcccac ctcgccaacg agggcaagga cgagcgactt
    25081 cgggtcatgg aactgcgccc agtctcgttc gtcgcggaag tcacggcact cgcgctggag
    25141 atcctccaag aggtatgccg cctgctcgtc acccatggca ccgatcgtag gcgcgctcat
    25201 cgcgcgacca cgctcctgca tgactagggt gaggcagtca gagcggatcg acgagcttcc
    25261 tcatccggac atgctccccc gcacgccatg tcgtgctgtc agggagcaac cccgtgtcgt
    25321 cgctgcttcg ccattccgcg gcggggtttt cagtgctcga gtcgcacctg gcggatcgtc
    25381 tcagcgagca actcgtgata gccgccggcc atcgaccctc gccggctgaa cgacggtcct
    25441 gggagcgaag tatcccggcg ttggtggcag acctgatcga tgctgaccgg ggcggtgtcg
    25501 aactcctcgt cgaataccaa ctgcccctca ccagcaagcg cgccgacgtc gtgctcgctg
    25561 gtgtcggagc gacgaccggg gctgcggcat acctggtcgt ggaactgaag cagtggaccg
    25621 aagccgcgcg tttcgaggac aacgacgtct tggtcagcgt tcccggttac gggcgcccgg
    25681 tcctccaccc gctcgaccag gtgcgcggct actgcgagta cctccaggac tacaacgccg
    25741 cacttgccga tgctccggat tcgttggcgg gaatcgctta cttacacaat gcgaccgatc
    25801 tcggtgtcac ggacctgttg gttcgtcgcc gcccgggggt acggctcttc acaggacagc
    25861 gtcgcagcga gtttcgttcc ttcctccggt cctacttcgc ccccgcaagt ggggctgccg
    25921 ccgctgacac gctcctcgcg agcagagtgg ctccctcgaa acaactcatg gcccttgccg
    25981 cccacgaggt ccagcgacgc gagatgttcg tgctgctcga cgagcagcgg gaggccttcg
    26041 agtgcgtgcg acacgccgtc ggtagtgcgc gacgggccaa ccacaagacg gccgtggtgg
    26101 tttcgggagg cccagggtcg gggaaaagcg tcattgcgct ctccctcatg ggcgaactgg
    26161 cccgcgaggg tcgcacggtg gtgcacgcga ccggatcgca gtcgttcacg cagacgttgc
    26221 gcaaggtggc ggccaagggg tcgccccggg tgcgtaaggc gttccagtac ttcaactcct
    26281 tcatggagtc agaacccaac gacctcgacg tgctgatcct ggatgaggct caccggatcc
    26341 gagagacctc ggcgaaccga tatacccgtg ccgcgcaacg gacgggtcga gcccaggtcg
    26401 acgagttgct ctctgccgca cggactcccg tgttcctcct cgatcagaac caggtcgtcc
    26461 gaccgggcga gatggggacc gttgccgata tcaccgctgc ggcagccgcc agggacattg
    26521 acgtcatcca cattcaactc gacgaccagt tccgatgcgg agggtcggcc gtctacgtgc
    26581 agtgggtcga acgactcctc ggcctcgtgc ccggcggcac tctgccatgg gtcggagatg
    26641 acggcttccg ggtgcgcgtg atggagtcac caagcgagat cgacgcgtgg ctgcaggcga
    26701 gggctgccga gggttacggg gcaaggatga ccgccggcta ctgctggccg tggagcgacc
    26761 cgaaccgcga cggcaccttg gtacccgatg tcacgatcgg cgactggtcg cgcccatgga
    26821 atctcaaggg ggaccgcgcg gtcggtggcg cgccaccgtc acctctgtgg gcgacggacc
    26881 ccgcggggat ggggcaggtc gggtgcgtct acaccgccca agggttcgag tacgacttcg
    26941 caggcgtgtt gctgggcccg gatctcgtgt ggcgcgacgg cgactgggta gcggtgcgat
    27001 cgggtaacaa ggaccccgcg ctgcgcagcc gaacggccgt gtcggatgat cacttcgatg
    27061 ccctggtccg caacatctac aaggtcctgc tcactcgcgg catgcagggc gtgggcatcc
    27121 actcggtcga cccggagacc aacgaactcc ttgccagcct ggtgcggcag tcgatcaagg
    27181 gctgacttcg atcacaccgt gggcgccgcg ctcggcatca accatcacgt ggctgaccac
    27241 cgggtagtgc ccggcctccg ggaaggtgag ttcgacgaac ccgccctgcc ccggagcaag
    27301 gtcgatgatc tggctgccgc ccggggcgtc cggcccccca ggtgcggcgc gcagcagcca
    27361 ggcgccctcg gcatacaccg tgtcgaactg gccacccacg atgtggaacg acgtgggtct
    27421 gtttggcccc gcgtcgagga cccagatgcg gacgcgctcg ccgacccgcg cggtgagggg
    27481 gcgagcgtcg tactggttgg cgacgccgtt gaagaccacc gcgtcgggtg tctcggcgtc
    27541 gattctggtc atctcgacct cggacgccga gccctgcgcg tggttgggcc cgaggtaaac
    27601 ctcggactgc acgaggacgt actcccggtc gaccggcgcc agcccaggtg ggtcgatgat
    27661 gactgcgcca tgcaggccgg ccgcgatgtg cgccgacatg ggcatcgtcg agcagtggta
    27721 catccagatc ccagagcggg tggcggtgaa gcgatagacg agcgatgcgc ccgtcgggat
    27781 ggtgcgcatc ggcttgtcgg gagcgagggc gccggcgtgg aagtcgatcg aatgccccat
    27841 cgtgccgtcg ttgacgaggg tcacctcgaa ggtgtcgccg accttgccgc gcagggtcgg
    27901 gcccggcacg accccgttga aggtccagcg cttctgccac accccggggg cgacctgcag
    27961 gtcaatctcg ctgatggtca aggtgattcg gtgcgtcgtc gaggccgcgg tcgaggcagc
    28021 gggcgcaggc gccaggggtg gaacggcgac aaagtcggca gggaagggcg cgtgcagatc
    28081 gacgccgctg ccgggcgact gcccgggtga ctgcccgggc gactgtcggg gtgcctgccc
    28141 aggcgactgc ccgtgccccg acgcggcccc cgtggtcgcc gccgcggacg cagccggggt
    28201 ggcgacgccg acgacggtca cagcgaagcg catccccatc tgccggtgcc cgaccaccga
    28261 gcaccagccg tccatcgacc cgccgaccac cccgacgtca agcgtggtgg aggcccccgg
    28321 gcgcagcctc ggcgtgccga gcccgttggc cagcacgagg tcgtggctcg tcgtcgggtc
    28381 ggcgttgatg agctcgatga ccagccgatc gcccgccggc acggtcacgg caggggggtc
    28441 gaaacgcatg tccatcgcag tgactcgcac cgtggtcgtg tgaccggtcg ccgcaaccgg
    28501 cgctgccccc gccgaagccc cggccccgaa ctccccggcc gcgactgtca gcccagcggc
    28561 cgccgggtcg gcgaggacgc ctaggcagac cacgaaggcg agcgccgagg ccccaccgac
    28621 gaactgccct gacgaccaca ccgaccgacg cttgaggtcg gtgcgcggaa cgggcacgtc
    28681 gcgcccacga ctctcggccg ccttccgcgc ccggatcgat gcgcgaatcg ccagccccag
    28741 caacggcaca aacatcccgt atgccgtgag caccagtgcc gtggtcagca ctcggacaat
    28801 gctcggcacc ggcaacaggc tcacgatgac accaccgttg atgacgacga gccgcacgag
    28861 ggcccacttg tccacccagg ccgaggcggc gcggaccgcc gctggaccgc ccccgaggac
    28921 ggcgggcacg aggtaggaca tcgctccgac cagtagttgt ggtgcgaacg cgctggccaa
    28981 cacggccgtc gggacgccgt aggtgtcgcc gatctcagtc caatcggcgc gcagcgcaac
    29041 ggtcccggcg acccatccga tggcgacgac agcccacgtc agggctgccg cgaccgagag
    29101 gggggcgagt tcgcccggcg ggcgagcccg cagtggggcg atgagcgccc ggccccacca
    29161 ggccccggcc agcaggtagc cgaccagccc gacgaccgtg accacccgca gacccgcggt
    29221 ggcgccgaca aggatgacga tgagagcaac ggcgaaaccc gggagcgcct gctgcgcgaa
    29281 gcgttcggct cgagggtcca tgcgggcgcg aagcatcgtc ggccacaggg tgacaagcgt
    29341 gccggtgacc gtgaggccga tccagccgag tgacatgacc atcgtgtggg cgacgagcaa
    29401 gcgtccgtgc cattcgtcct cgggcccgcg agccagcagg acgcccagcg tggcgccgac
    29461 cggaacccag gcggccgcag cgaggtagta gtagacgctg atccggaatc ggggcgcgat
    29521 cgacgcgcgc agcctgcgcc agagcatgac gccgtgccag atgaccgcga cgctcaccag
    29581 gaccgccccg gccagggccg accaccagat ctcggcggcg actccgacga ggaccaggct
    29641 caccccgacg aacagcagcg cgagccgttg gctctgggtc cgccgttcgt ccagacccgg
    29701 ccgggtcttg agcagcgcct gcgcgaagtg ggtgctccac accatggcgg catgggtgag
    29761 agcgccgagc aggacgaggt gggccatcaa ccaaccggcg tgaggaacca tccagtggat
    29821 gagcgcgacg acacccgcgg cgaccagcca gaacaacgac ggatagtccc gccaccccca
    29881 cgtcgcacgc cgcttcatga gcgacccccg gcactccgtg ctcgttgtcc cgatcgcgcg
    29941 ctccacacag cggtcccggc gaaggccagc agggcgacga cgttgagcac ggcaccgatc
    30001 tgccacgggc cctccagccc gagtccgtct cccgggccga tccgcacgat gaggccaatc
    30061 tgcaagagca ccaagggatt ccacattgcc gcgcgatagg gcagcggtcg cctcagcacc
    30121 gacgggaaga tcaccggagc gtgtgccatc accatcgaca tcgcgaagcc caggaaggtg
    30181 gcatggacga ccgtgtcgta tgccgcgagc gcgcgcggca tacctccgac gacccagacc
    30241 accgccgcaa cggcgagcca cacgtagccg cacagcagtg ccgccgcgga gaagcgcggc
    30301 aaccccgtcg agcggatcgt gcgccgggcg acgtcggagc gcgccagcca ggccaccaga
    30361 ccgagggtga ggaggccgaa ggcgcgggtg ccgacgctcg gccagaggag cgtcagggcg
    30421 gcggtcatca gcacggctgc accgatggcg aggaggaggc gccctgcgtg tggcggcatg
    30481 gtgagccggg ccaactcgac ccgctcggcg gcgatcgtga gcgcgatgta gccgacgagc
    30541 cacggcagta ggtcgacgac gtcaagccgg gtccacagga tcgcggcgcc caaagccatt
    30601 gcggcgccga ggatttgggt ggctgttgga tcgtcttcct ggcgccgcca tagatggacg
    30661 aggaccgcca ttgccaggcc acaaccgacg atcagcagaa ggc
//
LOCUS       DAOMZY010000008        30001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_1199_length_30001_cov_6.257898, whole genome shotgun sequence.
ACCESSION   DAOMZY010000008 DAOMZY010000000
VERSION     DAOMZY010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 30001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30001
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1199_length_30001_cov_6.257898"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="PLK64_01040"
     CDS             <1..597
                     /locus_tag="PLK64_01040"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPV78354.1"
                     /translation="WLDLAYQLTGIGLALMPVALCLYLLARVEPPEPGTAAYRVLGLD
                     LRRPRLDLGGGVVLAAAIGIPGLGLYLVATKLGFNTQVAPANLAGAWWTVPVLVLSAL
                     QNAVLEEVIVVGYLLTRLEQMGVGARWAVGISAVVRGSYHLYQGFGGFVGNIVMGVVF
                     ALVWRRWRRVGPLVVAHALIDIVAFVGYALLKDHLPWG"
     gene            complement(620..1549)
                     /locus_tag="PLK64_01045"
     CDS             complement(620..1549)
                     /locus_tag="PLK64_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPV78355.1"
                     /translation="MPPAPARENLTRFAWLSIAAAVATIALKFGAYLLTGSVGLLSDA
                     AESVVNLVAAFVALIALHVAAQPADVDHQFGHSKAEYFSAAVEGVMIFVAAIFILWSS
                     VQRFFAPEPLENVGMGLGISVAASALNGVVAFVLMRAGRKHNSITLTADGKHLLTDVW
                     TSVGVVVGVLLVVLTGWLRLDPIIAFLVGCNIIWTGWHLVRESVDGLMDHAPSPECQA
                     DIQAILDEFAHREGSPVLFHGVRARVAGHVQHVAFHVLVPGTWTVTQGHDLLEEIEGR
                     FHEKFGHIVVDTHLEPIEDPRSYDAPQWESASP"
     gene            1595..3166
                     /locus_tag="PLK64_01050"
     CDS             1595..3166
                     /locus_tag="PLK64_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HPV78356.1"
                     /translation="MTSEEQLRRRLLGDPPPRDLLGWLGPLVAMILGGIIRFWDLARP
                     HQLVFDETYYVKQAWSMLEWGVEMRNSSAFGDKPDEAFTHGTPNVFNSTDGDLVVHGP
                     VGKWVIASGEAMFGGDSSFGWRFAVCVLGTISIFMLGRIARRLLGSSVLGTIAAVLLA
                     FEGHHFVHSRTGLLDLILMFFALGAFGALLLDRDRSRAVLARRMAAASPDRLRDHRWL
                     GPSMGWRPWRWVAGLSLGLAAGTKWSGLYFLVVFGLMTVFWDLGARRAVGVRAYGLAT
                     VVRDGIPAFFTVVPTVAVTYLVSWWGWFVSDHGYNKAWAATHPASSTWAWVPGDLRSW
                     WNYQAQILEFHRGLSSPHSYQSSPWSWLVMGRPTSMFAEYPKLGEDGCTVDMCAKVIT
                     PIGTPTIWWLGVAAVVILVFQWALRRDWRAGAILAGYAGGYLPWFLVGDRTIFQFYAV
                     AFVPYVVLAVTYVLGMIAGRRDAPWRRRQIGAGVVGGFLVLTVLLFAWFWPLYTAQVI
                     PYSQWWLHMWFPSWV"
     gene            3260..>4150
                     /locus_tag="PLK64_01055"
     CDS             3260..>4150
                     /locus_tag="PLK64_01055"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HPV78357.1"
                     /translation="MTGTALRGVWVAIIAVLALAGGCGLDGPATVGPSTSAGVGPASA
                     PTTASALTTPGENLVPAGHGLALSILPVGSSEQAITVDGRIRMYRAYRPARLARTAPL
                     VVMFHGGLGSARQAESAYGWDTLADREGFVVLYPDGIGAAWNVGGGCCGLAPAQGVND
                     VAFVRAAIDNLRTRVSLDPGRTYAAGMSAGGMMAFRMACDTTLFAAIGPVAATQLGDC
                     PNPAPTSVMQVHGTADPTVPFEGSGTSASGSGSAPGSGLEPGLEPEPGIGPGIAQIVG
                     PAIPTLHATWRGIGRCGGDVI"
     assembly_gap    4151..4250
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4251..4966)
                     /locus_tag="PLK64_01060"
     CDS             complement(<4251..4966)
                     /locus_tag="PLK64_01060"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HPV78358.1"
                     /translation="MSQPATVVVQAADVEDVHARTAAVCARLARAHAELVEITREVLE
                     AELWAEGGIRSPQHWLIVRAGLSPSRAREIALLAARSDELPETLSAMSDGRLGIDQAA
                     VVARYVPAAYSAQASELAELATVTQLRRSLARYAFADEAGDSPDQASTEDTKSTEEAP
                     HSGPETERPRLSMSTVDGRFELSYSAPASVGALVETAIREARDSLFHAGLVDVSLADG
                     LAQVANRSLSAVEGTSRSAK"
     gene            complement(5125..5197)
                     /locus_tag="PLK64_01065"
     tRNA            complement(5125..5197)
                     /locus_tag="PLK64_01065"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5162..5164),aa:Ala,seq:cgc)
     gene            5305..6423
                     /locus_tag="PLK64_01070"
     CDS             5305..6423
                     /locus_tag="PLK64_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPV78359.1"
                     /translation="MSSTPSPIPAPVRAASEWAWRLLAIAGAVVAFAYLLGFVSEAVI
                     PVIVAVLLAAMLNPVNQWLRARLGRPAPAAALTVLATILAIGALLFLVGSQLTGGFPQ
                     MAAQVGTGIETIKTWLQTSFKISDTQFTQYLDDAKNALQTSEGLRSGLTKAGLGASHV
                     VAGLFISLFALFFFLFEGARIWGWVVRLFPRTARVRVAGAGEVAWTQLTAFTRATILV
                     ALVDAVGITVVALLLRVPFAPAIGAIVFLGAFIPIVGALASGMVAVLLALVAHGPIAA
                     LLMLAGVLAVQQIESHVLQPILLGRAVSVHPLAVILAITAGVVVAGVVGALVAVPLAA
                     VVNAVVRQLTGSGAAPDEGAGSASGGGESDEDPALSPS"
     gene            complement(6442..7515)
                     /locus_tag="PLK64_01075"
     CDS             complement(6442..7515)
                     /locus_tag="PLK64_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78360.1"
                     /translation="MQPSSLIFVAIIAIWAAFLVQHWVRRREALSTARSVDRFSEAIR
                     LLDRTPARVSAEPRPAPQVYAALASPLVARSGGGSGAVVAAGSGAAAGVGSGSGAAAA
                     EVLAGPAPAGPRPTATAPNRRGPRLALWGRRVRGLSFLGLVALVPVTVVLSALSVLRW
                     TSVLVAVLSLVLGLVMLRYAAVHERTLAKVGRSMAASELSGQRAAAATGSAARPAPRR
                     RSAAPARRVVATPGGAAPAVAPAASAADAPAQETSESATATADQTPAAQAPAALTPAV
                     QVPAAAAVTIEPAGERASRRAAYAQYSADGTWEPVAVPPPTYTLKARAAHPQPEPAEV
                     TATPEPMVAESAADDTHVRAVGD"
     gene            complement(7607..8197)
                     /locus_tag="PLK64_01080"
     CDS             complement(7607..8197)
                     /locus_tag="PLK64_01080"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPV78361.1"
                     /translation="MSLRVADGLTPSLVLRPLHRRDQSAWEALRRDNTAYLSRWEPTP
                     PDGMIVRTGFGGYVRSLNRDARAGNIAPFAVEVDGELAGQVHLFGVMRGSLLSGAAGY
                     WLGERFAGQGVATRALAALIDYALGPFGLHRVEVNIRPENVASLAVVAHLKFREEGVR
                     ERYLHIEGDWRDHRTFALTVDDLGGLPTIERWHLPG"
     gene            complement(8215..8715)
                     /locus_tag="PLK64_01085"
     CDS             complement(8215..8715)
                     /locus_tag="PLK64_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HPV78362.1"
                     /translation="MSSAPPRRAVVIVASTRAADGTYEDRTGPTLVDALTTWGYAATG
                     PIVVSDGDPVGLALATALRDSPEVVLTTGGTGVSPSDRTPEQTRPFLDRELPGIPELI
                     RARGFAGGQPLAAISRGLAGVSGRTLVVNLPGSRGGVRDAIDVLGPILDHVVEQLAGG
                     DHEAVG"
     gene            complement(8712..9212)
                     /gene="moaC"
                     /locus_tag="PLK64_01090"
     CDS             complement(8712..9212)
                     /gene="moaC"
                     /locus_tag="PLK64_01090"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HPV78363.1"
                     /translation="MTDPVSEAAPGLTHVRADGSAHMVDVSAKPITAREASAAGRVEL
                     SPAAVAALRGGTVPKGDALAVARIAGLQAVKRTPDLIPLAHPVAVHAASVDLSVDDAG
                     VEIRATVRTADRTGIEMEALTAVAVAALALVDMVKAVDRHARITEIRVTAKSGGRSGD
                     WREPGS"
     gene            complement(9209..10429)
                     /locus_tag="PLK64_01095"
     CDS             complement(9209..10429)
                     /locus_tag="PLK64_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HPV78364.1"
                     /translation="MADRTVEQHLAAVLAVARPLPPAALPLGAAVGLVAAANVVCLVD
                     LPGFDNSAMDGYAVRAADLLGATPNTPVRLPVVADLAAGDAPARPVGPGEAARIMTGA
                     MVPDGADTIVKVEDTDAGMPVVAVFVETPLGSSIRRRGEDLPVGGPVLASGTVLTARH
                     VGLLAASGHATVTVHPRPRVAVVSTGAELIAPGKPLQPGQIHDSNSYLLAALVDAAGG
                     HTAYRGSIGDHPDEVRALLDRLATEVDVIVTTGGVSMGVHDVVKAVLRERGEVDFVTV
                     AMQPGKPQGFGVLGERRVPFFGLPGNPVSAFVSFEAFVRPLLRRLLGLEPALAPTTRV
                     RLTAALRSPAGRMQFARATVSRDPHDGRWLATPVAGQGSHFVADLAAADALLLVPAEV
                     THLQVGDEVESILL"
     gene            complement(10422..11825)
                     /locus_tag="PLK64_01100"
     CDS             complement(10422..11825)
                     /locus_tag="PLK64_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HPV78365.1"
                     /translation="MSAAGLAEAARRMHERGLDERAIAVFSDYYHQLELGAQGTIPED
                     SIEPLVDLTRLDGVGATEAERAQALGQVVVIKLNGGLGTSMGLAGAKSALSVRDGLTF
                     LDVIARQVLALRAQYAAPLPVIFMNSFRTHEETEAILAAYPDLPVRGLPLGFLQSAEP
                     KLRVDDLSPVDWPANPELEWCPPGHGDVYLSLARTGLLDALRGMGIRYAFLSNADNLG
                     ATCDPDIAAWLLREQVPYLAEVCTRTVNDRKGGHLARRRSDGRIVLRDNAMVVPGEEP
                     FFADETRHSTFHANNLWVDLDVVARMLDERAGVLGLPIIVNRKTVDPTDKSSTKVIQI
                     ECAMGTAIEAIDGSRAMHVPRSRFRPVKTTNELTLVRSDLFRFEPDWRVAATTERPDP
                     LIDLSSEFAFVDDLERLFPAGVPSLRECDSLRVRGDVTFGAGVVCRGDVLVQGPVAVP
                     DAAVLTAESAGASGSGG"
     gene            11901..12461
                     /locus_tag="PLK64_01105"
     CDS             11901..12461
                     /locus_tag="PLK64_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HPV78366.1"
                     /translation="MSLPEVRAAKTALRRAIRTGRRAQAAAGGREAAGEALADGALAL
                     AEDVRSRTGRVCRVAAYVALPTEPPTMAMLERLRAAGHEVLLPVLLADKSLEWDLDRT
                     WQGPEALASCDLVLTPGLAVDRSGRRLGQGGGSYDRALVHVRPDVDIVTLLWDDELLD
                     TDVPAEPHDRRVTAVLTPGRGLIRLG"
     gene            complement(12445..15087)
                     /locus_tag="PLK64_01110"
     CDS             complement(12445..15087)
                     /locus_tag="PLK64_01110"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HPV78367.1"
                     /translation="MTRAKVVRRTLLWVGALVVVALVAAGGFGASAVRRAWPQTSGTI
                     ALEGLGGTVSVARDERGIPTITADNATDLFRAQGFVAASDRFFEMDLRRHIGSGRLAE
                     LVGSTGLAADQAVRTMGWRKVAEAELPTLAPNTRLYLGAYADGVNDYLTKAGEPANLA
                     VEYPLLATQLPEYRVEPWSAVDSLVWLKAMAYDIKGNFADELTRGRLTGTLGAGLVGS
                     LYPAYPTTEHPPILSSDDWSPTTSSLQTILPSALTLPAAQPATKPVTPAALAPELAAA
                     LTDPIAADAMAQASALLAPLVPLLGRGDGVGSNSWVVGPKLTKSGKPLLANDPHLGVS
                     IPSIWSQVGLRCRTVSTDCPFNVSGFALAGVPGVIIGHNGSIAWGFTNLGPDVTDFFL
                     ERVTDTAYQLDGRQVALQVTTETIKVRGGADVPLTVRATGHGPIVSDVMPGVAEVGKS
                     PLPGATSALETYAVSLAWTGLVPSTSADAIFALDAAGDFEEFRAAAKLFAVPSQNLVY
                     ADTSGNIGYQAPGLIPLRRSATAGLPAGFLPSPGWVSQGDWRGWVPFEDLPWALNPAE
                     GFIVAANQQVTAATSPFLTTEWDAGWRSQRIRERILAAGAGTLTAADLASIQGDSTDG
                     FAEKLVPALLAVDLADDPFTSSAQDLLRSWDLTTPADGSPSSAAAAYFYAVWTKLLQA
                     AFDDQLPVDLRADGGSRWRLVISTLLADPTNPWWDDKRTPSIVESRDEILRQSLVQAR
                     LALTKSLGSDVQGWSWGRVHTLTLRHRVLGADTVAAPIRALVNRGPFALPGGSAVVNA
                     TGWNASVGFAVNWAPSMRMVVDLGDLDASTWVNQSGQSGHPASPHYADQLGAWADGTT
                     FPWPFSDTALSTARREELLLTPAG"
     gene            15125..16729
                     /locus_tag="PLK64_01115"
     CDS             15125..16729
                     /locus_tag="PLK64_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HPV78368.1"
                     /translation="MSPRLASIAGIDVPGLAAAYGLADLTRSLLVGAAVLLLALVAVR
                     VSARSGLPTMLLYLAIGFALGEDGLGLAFDDARLTQVLGYSALVFILAEGGLTTSWPG
                     IRKSVAPAAMLSTVGVLVSVGAVAAAGHLLLDLSWPVALLVAAILSSTDAAAVFSVLR
                     SVPLPRRLAGLLEAESGFNDAPVVILVTALSLRLTDADQLPWWALLLTAVLELAAGSA
                     IGLAIGWLGGRVVRRLAGGASGLFAIGVLTVVVLAYAAAASVHASGFIAAYLAALVLG
                     NMGLPHRPAVHGFAEALGTLAQIGLFVLLGLLASPSRLPAQIVPAVVIGLVLLVFARP
                     LSVFVSLTPFRIGWRDQVFLSWAGLRGAVPVVLATVPLTVGAMGTQWIFDLVVVLVVV
                     YTLVQAPTLAWVARRLGVVESVSQTSIEVETTPLEELNADLMSVSIGPESRLHGVEIF
                     ELRLPPGAAVTLVVRGSETLVPIPSTRMRRGDQMLIVTTADVRRQVEKRLIMVSRHGR
                     LAGWVSDSTGPTGPGHGNSGTRGTVP"
     gene            16772..17200
                     /locus_tag="PLK64_01120"
     CDS             16772..17200
                     /locus_tag="PLK64_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017823870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPV78369.1"
                     /translation="MPTYAYACTACDHAFDAVQSFSDDPLSVCPECDGRLRKVYGSIG
                     VTFKGSGFYRTDSRAASSKSSTVPASSGGSGSGSSGSGSSGSGNGSSSSGSGSGSSGS
                     SSGSGSSGSSPGSGSPGSSSSGSGSSGSPSGSSSSSSASS"
     gene            17294..18124
                     /locus_tag="PLK64_01125"
     CDS             17294..18124
                     /locus_tag="PLK64_01125"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HPV78370.1"
                     /translation="MTSVPQAQIGVIGGSGFYSFLTGATRVTVATPFGEPSDDLVVGE
                     LHGRSVAFLARHGHDHRFPPHRVNYRANLWALRSLGVRQVIAPCAVGGLRPQLGPGTV
                     VVPDQLVDRTWGRAQTIYDDVGAVVHVSFADPYCPAGRAAVAAALADTDIPHQLGGTL
                     VVVNGPRFSSRAESLWHQSQGWDLVGMTTMPEAAIARELAMCFTTVAMVTDLDAGVEH
                     GDGVTHDEVLAAFAENMPRFKALVGDVVGRLPEPTPDETATCACRRSLDGLTLPFALP
                     "
     gene            18132..18830
                     /locus_tag="PLK64_01130"
     CDS             18132..18830
                     /locus_tag="PLK64_01130"
                     /inference="COORDINATES: protein motif:HMM:NF020250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HPV78371.1"
                     /translation="MSPVLSPAQGGALRPARRSRRQRFLLTRARRALTALLVGVAGWV
                     AVSAARPAPVEGGIPVLVAVRDLAAGARVGSGDLRAVRVPPDAVPARAILDASAAAGA
                     LLAGPLSEGEVLTATRLQGPGLLAGASSDTVAVAVPLADPSILAALRPGDRVRVLAVG
                     TGAVVGDATVLVATAPADEVSGLVATGSGPGSLIAALDRQAASALAAAQGPSGVSGGF
                     VIALLNSDTIRAPM"
     gene            19012..19377
                     /gene="mscL"
                     /locus_tag="PLK64_01135"
     CDS             19012..19377
                     /gene="mscL"
                     /locus_tag="PLK64_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HPV78372.1"
                     /translation="MLKGFKDFLMRGNVVDLAVAVIIAGAFGKVIEAFVTIVMDIIGK
                     LGGQPDFSAWAPGGVHLGSFITVTISFLIIAAAVYAIVVVPLNKLAERRKAGEVVEDE
                     APSAEVALLTEIRDALRTR"
     gene            19587..20309
                     /locus_tag="PLK64_01140"
     CDS             19587..20309
                     /locus_tag="PLK64_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPV78373.1"
                     /translation="MTWLVLGIALIAAFSAFTQIITGFGFALVGLPLLALVTDPVTAV
                     VSMTVLSTALTLGASIQQRRDIEWRPAGSVTATALLGMPVGLLALRLVDARWLSVLIG
                     IVLLAFTFALVRGIRLQGRAVTPTAGFVSGALLMSTGMNGPPLVVAFQNKEMSPPQFR
                     ATLQAVLTIQGFFGLVGFAAVGLFSPPVWSVVAYGAPAMLVGWWVGHLVSRRMDAALF
                     RRLVLVTLAASAATSIATGLLG"
     gene            complement(20337..24353)
                     /locus_tag="PLK64_01145"
     CDS             complement(20337..24353)
                     /locus_tag="PLK64_01145"
                     /inference="COORDINATES: protein motif:HMM:NF037405.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HPV78374.1"
                     /translation="MSVAQTIASWARELSEVGGRNTLLWAPEGPDRDGYLDLTTAHPG
                     GVSMLLAGRPTSLSDLVREAAAFEEARDTVRAIHAKASELLNERGLVVGFVAIGVATW
                     DPPRARAIVEAPVLLRTCTLRPTSPAQHDFDLDLGPTVEVNPVLVNYLRSVAGIAVDP
                     TALAALTTLKGSAGGFDPYPVYAALGRLCADLPGFAVTPRLVLGTYPYGKPDMVADVS
                     GQAQWLAQVDLVAALAGDEPAITRLRQALPEPAETPDPELELRVLDLDATQESVIEAV
                     RAGRQLVLTAPTGTGATQTVAALVAALAHDGKQVLYVTSQRRSLRELQDRLASVGLDD
                     LVLDLTGAGEDRRAVTSELGQALARLATLDDRDLADAADPRGRLDRAATVGQSEQTLA
                     DHVAAIHEVRRPWGVTAYEIQEAMGALAARHPAPGSRVRIGGAALEELSRARVTELAE
                     HLQAAAAAGAWSAEGGDPWYGATIRSEAEVERARDIVTRLTGGDLAATTADLDSILVE
                     SSLRPARSAADWRHALVTMRGVHETLEVFRPEIFDVPLDEHVVATGSRAYRAESAVEL
                     GGWSRSRVRRQARRMLRPGRPPADLHAELVRARNQRIAWHDLVGAGGRPEISPRLEEG
                     EAAYQGLVADLTWLDERLRPGPDDGPMLTTSFPLLRARLTRLAERLDRLDILPQVTAV
                     VDELRAARMGEVMDDFAARGVAAEHVPAEVEHVWWASLGQEIRARDPRYANHDGGALR
                     LAADQLAGLDEVGRTSDAGRVRAVVDRRARQRAREHPRQTDLLHAQAGLGHGHLPFAA
                     LHREADRVIGALRPCLAVSPYAVAQVLAPGTAVDVVVLDDATTVSVAEAVSALSRGRQ
                     TLVIGRPDGAVPSAFRASAGTRPVTDPSAAPPSLYAAATAVLPERRLAWWHTDLDARL
                     ALLPPEGITHGAPSPLRVPALRLDHVEGTAAVLPGTDSAIEWTAAEVARVVELVLEHA
                     RHRRDQTLAVLTLTADMAHLVRDGVRDGIGRLDGDVAAGEFLAGVGVEPFVVAQVEEA
                     DGLLRDVVLLAVGYGRTPHGRVLHRFPALNLPGGEAALIAATARARQELVVVSTLRSG
                     DLDAGRLRATPAGRLVELLAHAEAGGIHEGESTTAQGGDPLMSYLASRLRQEGLTVQA
                     QMGVGPHRVELAVGHPSVADRWLVAVESDGPGYAALPGVRARDVLRPRQLRRLGWEPI
                     RVWSTDLYRDPAPEVVRVVAAVTATLRARNAQVAAAEALEPKAPEPAAEPEPSVAEPN
                     SAQETEAAAESKPTEAAKASEVGEVGEAAKPGKRKKLKRSSAERSADDTDTGWGERPR
                     SGDEHDRWLEEQRPPHWE"
     gene            24593..24665
                     /locus_tag="PLK64_01150"
     tRNA            24593..24665
                     /locus_tag="PLK64_01150"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24626..24628,aa:Arg,seq:cct)
     gene            complement(24946..25392)
                     /locus_tag="PLK64_01155"
     CDS             complement(24946..25392)
                     /locus_tag="PLK64_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78375.1"
                     /translation="MSTLVAGGELERVTPDAALAARLLADGTRHIESARSAERLSDLT
                     GAYQLAYDALRKAAAALLAVQGLRATSRGGHIAIQDAVQAQFGGPGSPFRSFGRIRRN
                     RNAYEYPDSDEAGPDAGDLEDALRVAEAALMAAHALLGSGQLGVWQ"
     gene            complement(25416..26012)
                     /locus_tag="PLK64_01160"
     CDS             complement(25416..26012)
                     /locus_tag="PLK64_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78376.1"
                     /translation="MQSPAPQLLPLLRSALQGEVLALILLNPEQEWSLTELANRVGAS
                     VSTAQREVSRAEDAGVVVSRRVGNTRLVRALPSPITQPLTELLLRSFGPPQVIAEEFA
                     SVGGIEGLYLYGSWAARYAGVPGRAPADLDVLVIGRPDRDELDDAAQRAYRRLGREVN
                     VTIRSLAWWRNRTDGFHAEVTSRPLVPIRSVPETGDAS"
     gene            complement(26198..28708)
                     /locus_tag="PLK64_01165"
     CDS             complement(26198..28708)
                     /locus_tag="PLK64_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HPV78377.1"
                     /translation="MTSQDEMVGATHAALIAVLTLEEKVRLLTGRDTWSTWPIERIGL
                     RSMVFSDGPSGVRGPLWDERSPSLNLPSATALASSWDPEIARRYGNVSAQEARRKGVD
                     VVLGPTINLHRSPLGGRHFEAFSEDPWLTAELAAAYVMGVQENGVGATVKHYVANEYE
                     TERFTASSEVDERTLREVYLRAFEKAVVEARVWVVMSSYNSINGVTASENDLLETPLN
                     SEWGFDGVVVSDWTAVRSLAAASASQDLAMPGPEGAWGAALVEAVRDGRVSEAAIDRK
                     VARLLTLAQRVGALESYAGGPADVGGLLRVEDGVAFAREAAAEGTVLLANDGVLPLDV
                     ARLQRVAVIGQNAVAARTQGGGSATVIPERVVSPWEGIVAALPDAEVTYRIGAVAAMG
                     ITALPLEEMTNPATGGPGVLVRFLDDAGVELMREDRFATSLVFFAGNAPTLTAAVLEL
                     SCRWTPTENGDVTVGFASIGRGQISVDGALVLDDVAVGDSTDVGANILDPPKAVAPVA
                     VSVTAGRPVHVVLELDLAIMQETTDGAFGITLGVAPSDTDPEARIADAVAVAAASDVA
                     IVVVGTNAQVESEGFDRSDLRLPGRQDDLVAAVAATGTPTVVVVNSGSPVELPWRDDV
                     SAIVLPYFGVQEMGNALADVLLGRVEPGGRLPTTWAASMADVPVLDVTPVDGKLEYAE
                     GIHLGYRAWLRAGLGDGLGDEPREVPRAAYPFGFGLGYTTWELRDLVVEEVAASGGDV
                     RVGVTVTNTGARRGKQVVQVYLARPESSVDRPVQWLAGHAVVRADAGESVAVEISVPA
                     RAFAHWQNGVGWAHEPGTFNVMVGTSVDDIPLSSTVTL"
     gene            complement(28735..29409)
                     /locus_tag="PLK64_01170"
     CDS             complement(28735..29409)
                     /locus_tag="PLK64_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV78378.1"
                     /translation="MPTDVMRAARPLVATGVVYELSDGRPVLDDVTFALAPGTMTALT
                     GPSGAGKSTLLWILAGALAPTSGTVMYAAAPVGDRVAAARAGIVLVPQGVALVTALTA
                     RENLVVPLLMHGVGAGEAHARADAALASVLLAESGNHLVEELSGGEQQRVGVARALAL
                     RHSVLLADEPTSELDGATRETIVALLRAEADAGAVVLLATHDPWCADQADAEYHLDEG
                     SLRRVR"
     gene            29498..>30001
                     /locus_tag="PLK64_01175"
     CDS             29498..>30001
                     /locus_tag="PLK64_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78379.1"
                     /translation="MLGALRYRRAQAVVVVVLSALVTACLVLAPLYTRALEQSTVTTL
                     LRAATPTESGIRLASFSSAEPGIALDPDDLAELIPSSIRARYGTAIAATSVGVRRMPL
                     GPEPGGVLLARDNACAHVTFVEGQCPSAAGEIAVSADQAKVYEHAIGTAVFVGEFDGE
                     HGCPDGVF"
BASE COUNT         4214 a        10605 c        10659 g         4423 t
ORIGIN      
        1 tggctggacc tggcctacca actgaccggc atcggcctgg ccctcatgcc ggtcgcgttg
       61 tgtctctatc tgctggcgcg agtcgagccg cccgagccgg ggacggcggc ataccgggtg
      121 ctcggtctgg acctgcgccg accgcgcctg gacctgggcg gcggggtggt gctggctgcc
      181 gcgatcggca tccccgggct ggggctctat ctggtggcga ccaagttggg gttcaacacc
      241 caggtcgccc ccgccaacct cgccggggcc tggtggaccg tgccggtgct cgtgctgtcg
      301 gccctgcaga acgccgtcct cgaggaggtc attgtcgtcg gctacctgct cacccggctc
      361 gagcagatgg gcgtgggcgc acgctgggcg gtcgggatct ccgctgtggt gcgcgggtcc
      421 taccacctct accagggctt cggcgggttc gtcggcaaca ttgtcatggg ggtcgttttc
      481 gccctggtgt ggagacgctg gcggcgtgtg gggccactgg tcgtcgcgca tgcgctcatc
      541 gacatcgtcg ccttcgtcgg ctatgccctg ctcaaggacc acctgccctg ggggtgagcc
      601 tgcgtcggcg aaggcttggt cagggagagg cggactccca ctgcggtgcg tcgtaggagc
      661 gggggtcctc gatcggctcc aaatgggtgt cgacgacgat gtggccgaac ttctcgtgga
      721 accggccctc gatctcttcc aggaggtcgt ggccctgggt gaccgtccag gtgcccggga
      781 ccaggacgtg gaaggcgacg tgctggacgt gcccggcgac gcgggcccgg accccgtgga
      841 agaggaccgg ggacccttcg cggtgagcga actcgtcgag gatcgcctgg atgtcggcct
      901 ggcactccgg cgagggggcg tggtccatga gcccgtcgac ggactcgcgc acgaggtgcc
      961 agccggtcca gatgatgttg cagccgacga ggaaggcgat gatcgggtcg aggcgcagcc
     1021 agccggtcag cacgacgagc aagacgccga cgacgacccc gaccgaggtc cagacgtcgg
     1081 tgagcaggtg tttgccgtcg gcggtgaggg tgatcgagtt gtgcttgcga ccggcccgca
     1141 tgaggacgaa cgccaccacg ccgttgagcg ccgacgccgc caccgagatc cccagaccca
     1201 tcccgacgtt ctccagcggc tcgggggcga agaaccgctg caccgacgac cagaggatga
     1261 agatggcggc gacgaagatc atcacgccct cgacggcagc cgagaagtac tcggccttgg
     1321 agtgaccaaa ctggtggtcc acgtcggcgg gttgggcggc gacgtgcaag gcgatgaggg
     1381 cgacgaaggc cgccacgaga ttgaccacgg attcggccgc gtcggacagc aatccgaccg
     1441 atccggtcag caggtaggcc ccgaacttca gcgcaatcgt cgctacggcg gcggcgatcg
     1501 acagccaagc gaagcgggtg aggttctcac gggcaggcgc gggcggcata ccggacagtc
     1561 tggcactgcc gccggagtgc ctacgatgtc gctcatgacg agcgaggagc agctgcgccg
     1621 gcggctgctg ggggacccac ccccacggga cctgctcggc tggctcggcc cgctcgtcgc
     1681 catgatcctc ggcgggatca tccggttctg ggatctcgct cggccccacc agctggtctt
     1741 cgacgagacg tactacgtca agcaggcctg gtcgatgctc gagtggggcg tcgagatgcg
     1801 caacagctcg gccttcggcg acaagcccga cgaagccttc acccacggca cgcccaacgt
     1861 cttcaactcg accgacggcg atctggtcgt ccacggccca gtcggcaagt gggtcatcgc
     1921 ctcgggcgag gcgatgttcg gcggcgacag ttcgttcggg tggcggttcg cggtctgtgt
     1981 gctcgggacc atctcgatct tcatgctcgg ccggatcgcc agacgcctgc tcggttcgtc
     2041 ggtcctcggc accatcgcgg cggtgctgct cgccttcgag ggccaccatt tcgtccactc
     2101 ccgcaccggg ctgctcgacc tcatcctcat gttcttcgcg ctcggggcct tcggggcgct
     2161 gttgctcgac cgcgaccgat cgcgggcggt cctggcgcgc cggatggccg ccgcgtcgcc
     2221 cgaccggctg cgcgaccacc ggtggctggg cccctcgatg ggctggcgtc cgtggcgctg
     2281 ggtggccggc ctgtccctcg gcctggcagc ggggacgaag tggtcggggc tctacttcct
     2341 cgtcgtcttc gggctgatga cggtcttctg ggacctcggc gcccggcgtg ccgtgggggt
     2401 ccgggcctac ggcctggcga cggtggtgcg tgacggcata ccggcgttct tcacggtcgt
     2461 gccgacggtc gcggtgacct acctggtgtc gtggtggggg tggttcgtca gcgaccacgg
     2521 ctacaacaag gcgtgggcgg ccactcaccc ggcctcgtcg acctgggcgt gggtgcctgg
     2581 cgatctgcgc tcgtggtgga actaccaggc ccagatcctc gagttccacc gcggcctgtc
     2641 ctcgccgcac agctatcagt ccagcccctg gtcgtggctc gtcatgggcc gaccgacgtc
     2701 gatgttcgcc gaatacccca agctcggcga agacggctgc accgtcgaca tgtgcgccaa
     2761 ggtcatcacc ccgatcggca cgccgacgat ctggtggctc ggggtggccg cggtggtcat
     2821 cctggtgttc cagtgggcgc tgcgtcgcga ctggcgggcg ggggccatcc tcgcggggta
     2881 tgccggtggc tacctgccgt ggttcctcgt cggcgaccgg accatcttcc agttctatgc
     2941 cgtcgcgttc gtcccctacg tcgtgctggc cgtgacctac gtgctcggga tgatcgccgg
     3001 acgccgcgac gccccgtggc ggcggcggca gatcggcgcc ggagtggtcg gcggattcct
     3061 cgtgctcacc gtgctgctct tcgcctggtt ctggcccctc tacaccgcac aggtcatccc
     3121 gtattcacag tggtggctgc acatgtggtt cccctcctgg gtgtgaacgc gcgccatggg
     3181 tgagcgaaac cggcataccg ggacgtatgc cgcgccacgc gccgcagccg tcacgcgatc
     3241 tcgctgacat gatgagtcca tgaccggcac cgcgctcagg ggggtgtggg tggcgatcat
     3301 cgccgtcctc gcactcgcag gcgggtgtgg cctcgacggc cccgcgacgg tcggcccgag
     3361 cacgtcggcc ggcgtcggcc cggcgtcggc accgaccacc gcgtccgcac tgaccacgcc
     3421 gggggagaat ctcgtgcccg ccggtcacgg gttggcgctg tcgatcctgc cggtcgggtc
     3481 cagcgaacag gcgatcacgg tcgacggccg gatccggatg tatcgcgcct atcgaccggc
     3541 ccggttggcc cggaccgcgc cgctcgtcgt gatgttccac ggcgggctcg ggagcgctcg
     3601 gcaggcggag agcgcctacg ggtgggacac gctggccgac cgtgagggct ttgtcgttct
     3661 ctaccccgac ggcatcggcg ccgcgtggaa cgtcggcggt ggctgctgcg ggctcgcacc
     3721 agcgcagggg gtcaacgacg tcgccttcgt cagggccgcg atcgacaacc tccgcactcg
     3781 cgtctcgctc gaccccggcc gcacgtatgc cgcggggatg tcggcggggg gcatgatggc
     3841 ttttcggatg gcttgcgaca caacgctgtt cgcggcgatc gggccggtgg ccgccacaca
     3901 gctcggcgac tgccccaatc cggcgcccac gtcggtgatg caggtccacg ggaccgccga
     3961 cccaacggtg ccgttcgagg gctcggggac gtcggcgtcg gggtcggggt cggctccggg
     4021 gtcagggttg gagcccgggc tggagccgga gccagggatc gggccgggca tcgcccagat
     4081 cgtcgggccg gcgatcccca ccctgcatgc gacctggcgc ggcatcggtc gatgcggtgg
     4141 cgatgtcatc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tacttcgccg
     4261 accgcgacgt cccctccacc gccgacaacg accggttggc aacctgggcc agcccatcgg
     4321 ccagcgagac gtcgacgagg ccggcgtgga agagcgagtc gcgcgcctcg cggatcgccg
     4381 tctcgacgag ggcgccgacg gacgcgggcg ccgagtagga cagctcgaat cgtccgtcca
     4441 ccgtcgacat cgacagccgg gggcgctcgg tctccggtcc tgaatgcggg gcctcttcgg
     4501 tgctcttggt gtcctccgtc gacgcctggt cgggcgagtc acccgcctca tcggcgaacg
     4561 catagcgcgc cagtgaccgg cgcaactgcg tcaccgtcgc caactccgcc aactcactcg
     4621 cctgcgcgga gtatgccgcg gggacatacc gcgcgaccac ggccgcttga tcgatgccca
     4681 gccggccgtc ggacatggcc gagagcgtct cgggcagctc gtcgctgcgt gcggcgagca
     4741 gggcgatctc gcgggctcga ctcggtgaca gcccggcccg cacgatgagc cagtgctgcg
     4801 gcgagcggat gccgccctcg gcccacagct cggcctccaa cacctcgcgg gtgatctcga
     4861 cgagctcggc gtgggccctg gccaaccggg cgcacacggc agcggtgcga gcgtggacgt
     4921 cctcgacatc cgccgcttgc accactactg tcgccggttg actcatacct caatcataca
     4981 agtgttcgag tccttcggca aggtgtccgg ccatcaattc acttcgggca caagggtttc
     5041 ggacgagact gcctgcccgg cgcaaacgac gaaaccccgc ccgaccacag gcgttcctgc
     5101 tggtcgaacg gggtttccat cgagtggagc tgaggggatt cgaacccctg accttctcat
     5161 tgcgaacgag acgcgctacc aactgcgcca cagccccaga tgcgtcggac actctaacat
     5221 caggcgccgc agtggcacca agccagcacc cccacagccc gggtatgccg cgagcgaacg
     5281 aggcgcacgg cataccctgt ggccatgtcg agcaccccct caccgatccc cgccccggtc
     5341 cgcgccgcga gcgagtgggc ctggcgcctg ctggcgatcg cgggcgcggt cgtcgcgttc
     5401 gcctacctgc tgggcttcgt cagcgaggcc gtgatcccgg tcatcgtcgc ggtgctgctc
     5461 gccgcgatgc tgaacccggt caaccagtgg ctgcgggccc gactcgggcg gcccgccccg
     5521 gccgccgcgc tcacggtcct ggcgacgatc ctcgcgatcg gggcactgct gttcctcgtg
     5581 ggcagccagt tgaccggcgg cttcccgcag atggccgcgc aggtcggcac cgggatcgag
     5641 acgatcaaga cctggctgca gacaagcttc aagatcagcg acacgcagtt cacccagtat
     5701 ctcgacgacg ccaagaacgc gttgcagaca agtgaggggc tgcgctcggg gctcaccaag
     5761 gccggactcg gggcctcgca cgtcgttgcc gggctgttca tcagcctgtt cgcgctcttc
     5821 ttcttcctct ttgagggtgc ccggatctgg gggtgggtcg tccggttgtt cccgcggacg
     5881 gcccgggtgc gggtcgccgg cgcaggagag gtcgcctgga ctcagctcac cgccttcacg
     5941 cgcgcgacca tcctcgtggc cctcgtcgac gctgttggca tcaccgtggt cgctctgctc
     6001 ctgcgggtgc cgttcgcccc ggcgatcggt gcgatcgtct tcctcggcgc gttcatccct
     6061 atcgtgggcg cgctcgcgtc cggcatggtg gccgtgctgc tcgcgctcgt cgcgcacggt
     6121 ccgatcgccg ctttgctcat gcttgccggg gttctcgcgg tccagcagat cgagtcgcac
     6181 gtgctccagc cgatcctgct cggccgagcg gtgagcgtgc acccccttgc ggtgatcctc
     6241 gcgatcaccg ccggcgtcgt cgttgcaggg gtcgtcggcg cgctcgtcgc agtacccctg
     6301 gccgctgtgg tcaacgcggt cgtcagacaa ctcaccgggt ccggcgccgc cccagacgaa
     6361 ggcgcgggca gcgcatcagg tggcggggag tccgacgagg accccgcgct gtcgccgtcc
     6421 tgatcggcgc actcggccgg gtcagtcgcc gacggctcgc acgtgggtgt cgtctgcggc
     6481 cgactcggca accatcggct cgggggtcgc ggtcacctcg gcaggctcgg gctgcgggtg
     6541 ggcagcgcgt gccttgagcg tgtaggtagg cggcgggaca gccaccggct cccaggtccc
     6601 gtcggcggaa tactgggcgt atgccgcgcg ccgcgaggcc cgctctccgg cgggttcgat
     6661 cgtgaccgcg gccgcagcgg gcacctgaac tgcgggggtc agcgcggcag gggcctgagc
     6721 ggcaggcgtc tgatcagccg tcgccgtcgc gctctcggag gtttcctggg cgggagcgtc
     6781 agcagccgag gccgcggggg caacggcggg tgcggcaccg cccggcgtcg cgacaacgcg
     6841 gcgagccgga gcggccgacc gtcgccgggg cgccggccga gcagccgacc cggtcgccgc
     6901 ggcggcacgc tgccccgaga gttcggaggc ggccatcgac cgcccgacct tggccagggt
     6961 gcgctcgtgg acggcggcat accggagcat gaccagaccg aggaccagcg agaggaccgc
     7021 gacgaggacc gaggtccagc gcaacaccga cagcgccgac agcacgacgg tgaccggaac
     7081 gagcgccacc agcccgagga aggacagccc gcggacgcgg cggccccaca gcgcaagtcg
     7141 ggggcctcgg cggttcgggg ccgtcgccgt ggggcgagga ccagcgggcg cgggcccggc
     7201 gagcacctcg gctgccgcag caccagaccc agacccaaca ccagccgcag caccagaccc
     7261 agcagcaaca acagcaccag aaccgccacc actccgggcc acgagcggcg aggcgagggc
     7321 ggcatacacc tggggggctg ggcggggctc ggcggacacc cgcgcggggg tgcggtcgag
     7381 caggcggatg gcctcgctga accggtcgac cgaccgcgcg gtcgagaggg cctcgcggcg
     7441 gcgcacccag tgctggacaa ggaaggcagc ccagatcgca atgatggcga cgaagatcag
     7501 ggacgagggc tgcacgcgac taccgtatgg acccctgacg gcaccccggc ggacgtggcc
     7561 cggtgtgtcg cggatgaggc gcgtgtgact gctgtgaaaa cgccgatcag cccggtaaat
     7621 gccaccgttc gatcgtcggg aggccaccga ggtcatcgac cgtcagcgcg aaggtccggt
     7681 gatcgcgcca gtccccctcg atgtgcaagt aacgctctcg caccccctcc tcgcggaact
     7741 tcaggtgcgc gacgacggcc aacgacgcga cgttctcggg ccggatgttg acctcgaccc
     7801 gatgcagccc gaacggacca agggcatagt cgatcagcgc cgcgagcgcc cgggtggcca
     7861 caccttgccc agcgaaccgc tcccccagcc aatagccggc cgccccggag agcagcgacc
     7921 cgcgcatcac cccgaagagg tggacctgcc cggccaactc cccgtcgacc tccacggcga
     7981 acggcgcgat gtttccggcc cgagcgtccc ggttgaggga gcggacatac ccaccgaagc
     8041 cggttcgcac gatcatcccg tcagggggtg tgggctccca gcgggagagg tatgccgtgt
     8101 tgtcccggcg cagggcctcc cacgccgact ggtcgcggcg gtgcaacggc cgcaggacca
     8161 acgacggggt cagcccgtcg gcgacccgga ggctcacggg ccatcgcccg gggctcatcc
     8221 gacagcctcg tggtcgccgc cggcgagttg ctcgacaacg tgatccagga tcggcccgag
     8281 gacgtcgatc gcatcgcgca ccccgccgcg cgagccgggc aggttgacca cgagggtccg
     8341 gccgctcacg cccgcaagcc cccgcgagat tgcggccagc ggctgtccgc cggcgaaccc
     8401 ccgggcccgg atcagctccg gtatgccggg cagctcgcga tcgaggaacg gtcgggtctg
     8461 ctcgggggtc cggtcgctcg gggacacccc ggtgccgccg gtcgtgagga cgacctcagg
     8521 tgagtcgcgt agggcggtcg ccaaggccaa gccgaccggg tcaccgtcgg agacgacgat
     8581 cgggccggtg gcggcatacc cccaggtggt gagggcgtcg acgagggtgg ggccggtgcg
     8641 gtcctcgtag gtgccgtcgg cggcccgggt ggaggcgacg atgaccaccg ctcggcgcgg
     8701 gggcgctgag ctcacgagcc cggctcccgc cagtcaccgg agcgcccacc agacttggcg
     8761 gtgacccgga tctcggtgat tcgcgcgtgc cggtcgacgg ccttgaccat gtcgacgagg
     8821 gccagggcag cgaccgcaac agccgtgagg gcctccatct cgatcccggt ccggtcggcc
     8881 gtgcggaccg tggcccggat ctccacgccc gcatcgtcga ccgacagatc gacggaggcg
     8941 gcgtgaaccg cgacgggatg cgcgagcggg atgaggtcgg gggtgcgttt cacggcctgc
     9001 agaccggcga tccgcgcgac ggcgagggca tcgcccttcg gcacggtgcc cccgcgcagg
     9061 gccgccaccg ctgcggggga caactccacc cgtccggcgg ccgaggcctc gcgggccgtg
     9121 atcggcttcg cgctgacgtc gaccatgtgc gcgctgccat cggcgcggac atgggtcagc
     9181 cccggcgccg cctcgctcac cgggtcggtc ataggaggat gctctcgacc tcgtcgccca
     9241 cctgcaagtg ggtgacctcg gcgggcacga gcagcagcgc gtcggcggcg gccaggtccg
     9301 cgacgaagtg cgagccctgc ccggccacgg gggtcgccag ccaccgcccg tcgtgcgggt
     9361 cgcgggacac ggtggcgcgg gcgaactgca tccggcccgc gggcgagcgc agcgccgcgg
     9421 tgaggcgcac ccgggtggtc ggcgcgagcg ccggctcgag cccgagcagc cggcgcagca
     9481 gcggccgaac gaacgcctcg aacgacacga acgccgacac cggattgccc ggcagcccga
     9541 agaacggcac ccgccgctcg cccagcaccc cgaacccctg cggcttgccc ggttgcatgg
     9601 ccacggtgac gaagtcgacc tcgccgcgct cgcgcaacac tgccttgacg acgtcgtgga
     9661 cccccatgct gaccccgccg gtggtgacga tgacgtcgac ctcggtggcc agccggtcca
     9721 gcagcgcgcg cacctcgtcg gggtggtcgc cgatgctccc ccggtatgcc gtgtgcccac
     9781 ctgccgcgtc gacgagggca gcgagcaggt agctgttgga atcgtggatc tgcccaggct
     9841 gcaacggttt tcccggggca atgagctcgg caccggtcga gacgacggcc accctggggc
     9901 gggggtggac cgtgacggtg gcgtgcccag aggccgcgag cagaccgaca tggcgggccg
     9961 tgagaacggt cccggacgcc agcaccgggc cgccgacggg gaggtcctca ccgcgccgac
    10021 ggatcgacga gccgagcggg gtctcgacga aaacggcgac gaccggcata ccggcgtcgg
    10081 tgtcctccac cttgacgatg gtgtcggcgc catcggggac catggctccg gtcatgatcc
    10141 gggccgcctc gccggggccg accgggcgtg cgggggcgtc gccggcggcc agatcggcca
    10201 cgacgggcaa ccgcacgggg gtgtttgggg tcgctccgag caggtccgcc gcgcgcacgg
    10261 catacccatc catcgcggag ttgtcgaagc cgggaaggtc gacgaggcag acgacattgg
    10321 cagcagccac gagaccgacc gccgcaccga gcgggagggc ggcgggcggc agcgggcgag
    10381 cgaccgcgag gaccgctgcc aggtgttgct cgaccgtgcg gtcagccacc ggacccgctc
    10441 gcgccggccg actcagccgt cagcacggcg gcgtcgggca cggcgacggg gccctgcacg
    10501 agcacgtcac cccggcagac caccccggcc ccgaaggtga cgtcgccacg cacccgcaag
    10561 gagtcgcact cacgcaggga gggcaccccg gccgggaaga gccgctcaag gtcgtcgacg
    10621 aacgcgaact cgctcgacag atcgatgagc ggatcgggac gttcggtcgt cgccgccacc
    10681 cgccagtcgg gctcgaaccg gaacaggtct gagcgcacga gggtgagctc gttggtcgtc
    10741 ttcaccggcc ggaaccggct gcgcggcaca tgcatcgccc gcgaaccgtc gattgcctcg
    10801 atggccgtcc ccatggcgca ctcgatctgg atgaccttcg tcgaggactt gtcggtcggg
    10861 tcgacggtct tgcggttgac gatgatcggc agtcccagca ccccggcgcg ctcgtcaagc
    10921 atcctggcga cgacgtcgag gtcgacccac aggttgttag cgtggaaggt gctgtgtcgg
    10981 gtctcgtcgg cgaagaaggg ctcttcgcca gggaccacca tcgcgttgtc ccgcaacaca
    11041 attcgtccgt cgctccggcg gcgggcgagg tgcccaccct tgcggtcgtt gaccgtgcgg
    11101 gtacacacct cggcgaggta gggcacctgt tcgcgcaaga gccacgccgc aatgtcgggg
    11161 tcgcaggtgg ccccgaggtt gtcggcgttg gagaggaacg cgtagcggat ccccatgccc
    11221 cgcaacgcat ccagcaagcc ggtgcgggcc agcgacagat agacatcgcc atggccgggc
    11281 gggcaccact ccaactcggg gttggccggc cagtcgaccg ggctgaggtc atcgacccgc
    11341 agcttcggct cggcgctttg caggaatccc agcggcagcc cccgcaccgg caggtcgggg
    11401 taggccgcga ggatcgcttc cgtctcctcg tgcgtgcgga aagagttcat gaagatcacc
    11461 gggagggggg cggcatactg cgcgcgcaga gcgagcacct gccgggcgat gacgtcgagg
    11521 aaggtcagcc cgtcacgcac ggacagcgcc gacttcgcac ccgccaggcc catgctcgtc
    11581 ccgagcccgc cgttgagctt gatgacgacg acctgaccga gcgcctgtgc ccgctcggcc
    11641 tcggtcgccc cgacaccatc gaggcgggtc aggtcgacca gcggctcgat cgagtcttcg
    11701 ggaatagtgc cttgggcgcc caactcgagt tggtggtagt agtcgctgaa gaccgcaatg
    11761 gcccgctcgt ccagaccccg ctcatgcatc cgtcgagcgg cttcggccag accggcggcg
    11821 ctcatttgat cctccagggg acggtgggca gagtcaccgc gccagcctaa cggcccgccc
    11881 cgcgggccta cgatccgatc atgagcctgc ccgaggtccg cgccgcgaag accgcactcc
    11941 ggcgcgcgat ccgcaccggc cgccgagccc aggccgccgc cgggggccgc gaagccgcgg
    12001 gtgaggcgct cgccgacggg gccctcgccc tcgccgagga cgtgcgcagt cgcaccggga
    12061 gggtatgccg cgtggcggca tacgtcgcgc tcccgaccga gccacccacg atggcgatgc
    12121 tggagcgcct gcgcgctgcc ggtcacgagg tcttactgcc ggtgctgctc gccgacaaga
    12181 gcctggagtg ggacctggac cgaacctggc agggaccgga ggccctggcc tcctgcgacc
    12241 tcgtgctcac cccggggctg gccgtcgacc ggtcgggtcg ccggttgggg cagggcggcg
    12301 gcagctacga ccgggccctc gtgcacgtgc gccccgatgt cgacatcgtc accctgctgt
    12361 gggacgacga actcctcgac acagacgtcc ccgccgagcc acacgaccgg cgggtcactg
    12421 ccgtgctcac gccgggccgc ggactcatcc ggctggggtg agaaggagtt cctcgcggcg
    12481 ggcggtgctc agtgccgtgt cgctgaacgg ccaggggaag gtcgtcccgt cggcccaggc
    12541 accgagttgg tccgcgtagt gggggctcgc gggatgcccg ctctggccgg actggttgac
    12601 ccaggtcgac gcgtcgaggt cgcccagatc gacgaccatc cgcatcgacg gggcccagtt
    12661 gaccgcgaag ccgacggagg cgttccaccc ggtggcgttg acgaccgccg agccaccggg
    12721 cagcgcgaag gggccgcggt tgaccagggc ccggatcggc gcggcgacgg tgtcggcgcc
    12781 gagaacccgg tggcgcaggg tcagcgtgtg gacccgcccc caggaccagc cttggacgtc
    12841 cgagccgagc gacttggtga gggcgaggcg ggcctggacg agcgactggc gcaggatctc
    12901 gtcgcgactc tcgacgatcg acggggtgcg cttgtcgtcc caccacgggt tggtcgggtc
    12961 ggccaggagg gtcgagatga ccagccgcca gcgcgagccg ccatcggcgc gcaggtcgac
    13021 gggcagttgg tcgtcgaagg cggcctgcag cagtttggtc cacaccgcat agaagtaggc
    13081 cgcggctgcg gacgacggtg atccgtcggc aggggtggtg aggtcccagg agcgcaacag
    13141 gtcttgcgcg ctgctggtga agggatcgtc ggccaggtcg acagcgagta gtgcggggac
    13201 gagtttctcg gcgaacccgt cggtgctgtc accctggatc gaggcgaggt ctgcggcggt
    13261 gagggtgccc gcgccggcgg cgaggatccg ctcacggatg cgctgactgc gccagccggc
    13321 atcccactcg gtggtgagga acgggctcgt cgccgcggtg acctgctggt tggccgcgac
    13381 gatgaacccc tcggcggggt tgagcgccca cggcaggtcc tcgaacggca cccagccgcg
    13441 ccaatctcct tgcgacaccc acccggggct cgggaggaat cccgcaggca gtcccgccgt
    13501 cgccgagcga cgcagcggga tgagccccgg cgcctggtag ccgatgttgc ccgacgtgtc
    13561 ggcatagacg aggttctgcg acggcaccgc gaagagcttg gccgctgccc ggaactcctc
    13621 gaagtcaccg gcggcgtcga gcgcaaagat cgcatcggcg ctcgttgagg gcaccagccc
    13681 ggtccaggcg agggacacgg catacgtctc cagcgcggag gtggccccgg gcagcggtga
    13741 cttgccgacc tcggcgaccc caggcatgac atcgctgacg atcggtccgt gtccggtggc
    13801 gcggaccgtg agggggacgt ccgccccgcc gcggactttg atcgtctcgg tcgtcacctg
    13861 gagcgcgacc tggcgtccgt cgagttggta tgccgtgtcc gtcacccgct cgaggaagaa
    13921 atcggtgacg tcgggcccga ggttggtgaa gccccaggcg atcgagccgt tgtgcccgat
    13981 gatcacgccg gggacgccgg cgagggcgaa gccgctgacg ttgaacgggc agtcggtgct
    14041 cacggtgcgg cagcgcagcc cgacctggct ccagatgctg gggatcgaga cgccgaggtg
    14101 cgggtcgttg gccagcagcg gcttgccgga cttggtgagc ttgggcccga cgacccagga
    14161 gttggagccg acgccgtcac cccggccgag gagcgggacg aggggcgcca gcagggcgga
    14221 ggcctgcgcc atcgcgtcgg cggcgatggg gtcggtcagc gcggcggcca gctcgggggc
    14281 gagggcggca ggcgtcacgg gcttggttgc gggctgggcg gccgggaggg tgagggcgct
    14341 gggcaggatc gtctgcaggc tcgaggtcgt cggcgaccag tcgtcggacg acaggatcgg
    14401 tgggtgctca gtcgtcggat aggccgggta gagcgacccg acgaggcccg cccccaatgt
    14461 gccggtcagg cggccccgcg tgagctcgtc ggcgaagttg cccttgatgt cgtaggccat
    14521 cgccttgagc cagacgaggg agtcgacggc cgaccacggc tcgacgcgat actcgggcag
    14581 ttgagttgcc aacagtggat attcgacggc gaggttggcc ggttcgccgg ccttggtgag
    14641 gtagtcgttg accccgtcgg cataggcgcc gagatagagc cgggtgttcg gtgccagcgt
    14701 gggaagctcc gcctcggcga ccttgcgcca ccccatcgtc cggaccgcct ggtcggccgc
    14761 gaggcccgtc gaccccacga gctcggccag acggccggag ccgatgtgcc gtcgcaggtc
    14821 catctcgaag aaccggtccg acgccgccac gaacccctgc gcgcggaaga gatcggtcgc
    14881 gttgtcggcc gtgatcgtcg gtatgccgcg ctcgtcccgg gcgaccgaca ccgtcccgcc
    14941 cagcccttcg agggcgatcg tcccgctggt ctgcggccag gcacgccgca cggccgatgc
    15001 accgaagccg cccgcggcca ccaacgccac gaccacaaga gcaccgaccc acaacagggt
    15061 ccggcggacc accttggcac gcgtcaccct gcgagactaa gccacaccaa cggaaggacc
    15121 acggatgtcg ccccgcctcg cgtcgatcgc gggcatcgac gtgcctgggt tggccgcggc
    15181 atacgggctg gcggacctga cccggagcct cctggtcggg gcggcggtcc tgctgctggc
    15241 cttggtcgcg gtgcgcgtgt cggcgcgatc gggcctgccg acgatgctgc tctacctcgc
    15301 catcgggttc gcgctcgggg aggacgggct ggggctggcc ttcgacgatg cgcgcctcac
    15361 tcaggtcctg ggctattcgg cgctcgtctt catcctcgcc gaaggtggcc tgacgacctc
    15421 gtggccgggg atccgcaagt cggtcgcccc agccgccatg ctctcgacag tcggcgtgct
    15481 cgtgtcggtc ggggcggtcg cggcggcggg gcacctcctg ctcgacctca gttggcccgt
    15541 ggccctgctt gtggccgcga tcctgtcgtc gaccgacgcc gccgcggtgt tctccgtgtt
    15601 gcgtagcgtg cccctccccc ggcggttggc cgggctgctc gaagccgagt cgggcttcaa
    15661 cgacgcgccc gtcgtcatcc tcgtcacggc gttgtcgctg cgccttaccg acgccgacca
    15721 gttgccgtgg tgggcgctgc tgctcaccgc cgtgctcgag ttggccgccg gatcagccat
    15781 tgggctggcc atcgggtggc tcggtggccg ggtcgtgcgc cgcctcgcgg gaggcgcgtc
    15841 cggattgttc gccatcgggg ttctcaccgt cgtcgtcctc gcgtatgccg ccgcggcgag
    15901 cgtgcacgcc tccggcttca tcgcggcata cctcgcggcc ctcgtcctcg ggaacatggg
    15961 gttgccgcac cggcccgcgg tgcacggctt cgccgaggcg ttggggacgc tcgcccagat
    16021 cggtctcttc gtgctgctcg ggctgctggc cagccccagc cggctgccgg cccagatcgt
    16081 gccggccgtt gtcatcggtc tcgtgctgct cgtctttgcg cggccgctgt cggtgttcgt
    16141 ctcgctcacg ccgttccgga tcgggtggcg cgatcaagtg ttcctgtcgt gggcggggct
    16201 gcgcggtgcc gtgcccgtcg tcctggcaac cgtcccgctg acggtcggcg cgatggggac
    16261 ccagtggatc ttcgacctgg tcgtcgtcct cgtcgtcgtc tataccctcg tccaggcgcc
    16321 gacgctggcg tgggtcgcgc gccgactggg cgtcgtcgag tcagtgagcc agacgtcgat
    16381 cgaggtcgag acgactccgt tggaggagct caacgccgac ctcatgagcg tctccatcgg
    16441 gccggagtcg cggctgcacg gggtcgagat cttcgagtta cggctgcccc caggggctgc
    16501 agtgacgctc gtcgtgcgcg gctcggagac gctcgtgccg atcccgagca cccggatgcg
    16561 acgcggcgac cagatgctca tcgtcaccac cgcggacgtg cgtcggcagg tcgagaagcg
    16621 tctgatcatg gtcagccgcc acgggcgact ggccggctgg gtgtccgact ccactggccc
    16681 gactggaccg ggtcacggga attcgggcac ccggggcacc gttccgtaga ctggcccacc
    16741 gccacatccc gaagatcgac cgaggaccac cgtgcccacg tacgcctacg cctgcaccgc
    16801 ctgtgaccat gcgttcgacg ctgtccagtc cttcagcgac gacccgttgt ccgtgtgccc
    16861 cgagtgcgac ggtcggctgc gcaaggttta cggctcgatc ggcgtgacct tcaaggggtc
    16921 cggcttctac cgcaccgact cccgcgccgc ctcgtcgaag tcgtcgacgg ttcccgcgtc
    16981 ctccgggggg tcgggctctg gctcgtcggg ttctggctcg tcgggttctg ggaacggttc
    17041 gtcgtcgtcc gggtctgggt ctgggtcctc aggctcatcg tctggctcgg ggtcgtccgg
    17101 ctcgtcgccc gggtctggtt ccccgggctc gtcgtcgtcc ggctcagggt cctcaggctc
    17161 gccgtctggg tcgtcgagtt cgtccagcgc ctcgtcctga ggtcgtccac acccccctgg
    17221 ccggtcgtcc caccgtccac aggcacccga ggcgaggggg cggcggcata ccgcagcctc
    17281 taaccttcgg gacatgacga gcgttccgca ggcgcagatc ggggtcatcg gcggctccgg
    17341 gttctacagc ttcctcaccg gcgcgacccg ggtgacggtg gcgactcctt tcggggagcc
    17401 gagcgacgac ctggtcgtcg gtgagttgca tggccgcagc gtggcgttcc tcgcccgaca
    17461 cgggcatgac catcgcttcc cgccgcaccg ggtcaactac cgggccaacc tgtgggccct
    17521 gcgctccctc ggcgtccggc aggtcatcgc gccctgtgcg gtcggcgggc tgcggccgca
    17581 gctcgggccg ggcaccgtcg tggtccccga tcagctcgtc gaccggacgt gggggcgcgc
    17641 ccagacgatc tacgacgacg tgggcgccgt cgtgcatgtg agcttcgccg acccttactg
    17701 cccggccggg cgagctgcgg tcgcagcggc tctcgccgac actgacatcc cccatcagct
    17761 cggcggcaca ctcgtcgtcg tcaacgggcc acggttctcc tcacgcgccg agtcgctgtg
    17821 gcaccagtcc caagggtggg atctcgtcgg catgacgacg atgccagagg ccgcgatcgc
    17881 ccgtgagctg gcgatgtgtt tcacgacggt cgccatggtc accgacctcg atgcgggggt
    17941 cgagcacggc gatggagtga cccatgacga ggtcctcgct gccttcgccg agaacatgcc
    18001 acggttcaag gcgttggtcg gagacgtcgt cggtcggctg cccgaaccga cgcccgacga
    18061 gacggcgacc tgcgcctgtc ggcgctccct cgacggcctc accctgccgt tcgcgttgcc
    18121 atgagcccgc catgagccct gtcttgagcc ctgcccaggg cggtgcgcta cgtccggccc
    18181 gccgatcccg tcggcagcgg ttcctgctga ctcgggccag gcgggcactg acggcactcc
    18241 tcgtgggggt cgccgggtgg gtggccgtgt cggcggcccg ccccgctccc gtcgaaggcg
    18301 gcataccggt gctcgtggcg gtccgagacc tggcagcggg ggcgcgggtc ggatcagggg
    18361 acctccgcgc ggtgcgggtg ccgccggacg cggtcccggc gcgggcgatc ctcgacgcga
    18421 gcgccgcggc gggcgccctc ctcgccggcc cgctgagcga gggcgaagtg ctcactgcga
    18481 cccgcctgca gggtccaggc ctgctcgccg gggcgtcgtc cgacacggtc gcagtggccg
    18541 tcccgttggc cgatccatcg atcctcgcgg ccctgcgacc cggagatcgc gtccgggtgc
    18601 tggccgtggg cactggcgcc gtcgtcggcg atgcgaccgt cctcgtcgcg acggcgccgg
    18661 ccgacgaggt cagcgggctg gtcgcgacgg gttccggccc cggcagtctc atcgcggccc
    18721 tggaccggca ggcggcctcg gctctggcgg ctgcccaagg cccgtcgggg gtgtctgggg
    18781 ggttcgtcat cgcgttgttg aactccgaca cgatacgcgc gccgatgtga cctaattccc
    18841 attttgtgca cggacggagt agcctgaacg tcgtgctcgg ggaagctctc ctctggcatg
    18901 ccaagcgaaa cacttcatgc tcccctgggc gcacctgccg cccacgccac cctcaactga
    18961 ggctggatct cccagggtcg ggagcccacc ccacaaaagg atcacgcacc catgctcaag
    19021 ggattcaaag acttcctcat gcgcggcaac gtcgtcgacc tcgcggtcgc cgtcatcatc
    19081 gccggcgcgt tcggcaaggt catcgaggcc ttcgtcacga tcgtcatgga catcatcggc
    19141 aagctcggcg gtcagccgga cttctccgca tgggccccgg gcggcgtgca cctcggcagc
    19201 ttcatcaccg tgacgatctc gttcctcatc atcgcggccg ccgtctacgc catcgtcgtc
    19261 gtcccgctca acaagctcgc cgagcgccgc aaggccggcg aggtcgtcga ggacgaggcc
    19321 ccctcggccg aggttgccct cctcaccgag atccgcgacg ccctgcgcac tcgctgattg
    19381 caccacagca ccactggcac gacctgaacc acgggcacga cctgaaccac acgcaccacc
    19441 tgttcgacca cgaaacgccc cggcggggcg ggagccattg gcacccgccc cgccgtcggc
    19501 tttcacgggc gggtaggcct ctgggaccac gtgcccacat cgtccgaccg ggtgccaacc
    19561 tcacgctggg aaggcacact tgatgggtga cttggcttgt cctcggcatc gctctgatcg
    19621 ccgcgttcag cgcgttcacc cagatcatca ccggattcgg cttcgccctc gtcggccttc
    19681 cgctcctcgc cctcgtgacc gaccccgtga cggcagtggt ctcgatgact gtcctgagca
    19741 ctgcgctcac cctcggcgcc tcgatccagc agcgccgcga catcgaatgg cgcccggccg
    19801 gaagcgtcac ggccaccgcc ctgctgggta tgccggtcgg gctgcttgcc ctgcgcctcg
    19861 tcgacgcgcg ctggctcagt gtcctcatcg gcatcgtgct gctggcgttc accttcgcgc
    19921 tcgttcgcgg gatccgattg cagggtcgcg ccgtgacccc gaccgcgggc ttcgtcagtg
    19981 gcgcgctgct catgtcgacg ggcatgaacg gcccgcccct ggtcgttgcg ttccagaaca
    20041 aggagatgag cccgccgcag ttccgcgcca ccctgcaggc ggtcctcacg atccaaggct
    20101 tcttcggcct cgtaggcttc gccgccgtgg ggttgttcag cccgcccgtc tggtcggtcg
    20161 tggcctacgg agcgccggca atgctcgtcg gctggtgggt cgggcatctc gtctcccgac
    20221 ggatggatgc cgcgctgttc cgacggctcg tcctggtcac gttggccgcc agcgcagcca
    20281 cctcgatcgc caccggcctg ctcggctgag caggcccgag cggcatacct gccgggtcac
    20341 tcccagtgcg gagggcgctg ttcctcgagc caacggtcgt gctcgtcgcc gctgcggggc
    20401 cgctcgcccc acccggtgtc ggtgtcgtcg gcgctgcgct cggcagaact gcgcttgagc
    20461 ttcttgcgct tgcccggctt ggcagcctcg ccgacttccc cgacctcgct ggccttggcg
    20521 gcctcggtgg gtttgctctc cgcagccgcc tcggtctcct gcgccgaatt gggctccgcc
    20581 actgacggct caggctcagc ggccggctcg ggtgccttcg gctcaagcgc ctctgcggcc
    20641 gcgacttggg cgttccgcgc ccgcagcgtg gccgtcacgg cggcgacaac gcgcaccact
    20701 tcgggcgccg ggtcgcgata gagatcggtg ctccagaccc gaatcggctc ccagccgagt
    20761 cggcgaagtt gccgcgggcg caggacgtcg cgggcgcgga cgcccgggag ggcggcatac
    20821 ccgggtccgt cgctctccac cgcgaccagc cagcggtcgg cgacggaggg gtggccgacg
    20881 gcgagttcga cgcggtgggg gccgacaccc atctgggcct ggacggtgag gccctcctgg
    20941 cgcagccgcg aggcgaggta ggacatgagc gggtcgccac cttgggcggt ggtcgactcc
    21001 ccctcgtgta tgccgcccgc ctcggcgtgc gcgagcaact cgaccaggcg gccggccggg
    21061 gtcgcccgca ggcggccggc gtcgaggtcg ccggagcgga gcgtcgacac gaccacgagt
    21121 tcctgccggg cccgcgccgt ggcggcgatg agggcagctt cgccaccggg aaggttgagc
    21181 gccgggaagc ggtgcagcac ccggccgtgc ggcgtgcggc cgtagccgac ggcgagcagc
    21241 acgacgtcgc gcagcaggcc gtcggcctcc tcgacttgag ccacgacgaa cggctcgacc
    21301 ccgacgccag cgaggaattc gcccgcggcg acgtcgccgt cgagccggcc gatgccgtcg
    21361 cgaacgccgt cgcgcaccag gtgggccatg tcggcggtga gggtgaggac ggccagggtc
    21421 tggtcgcggc ggtgccgggc gtgttccagg acgagctcga cgacccgcgc gacctcggca
    21481 gcggtccact cgatggcgct gtcggtgccg ggcagcacgg ccgcggtgcc ctcgacatgg
    21541 tcgaggcgca gcgcggggac tcgcagcggc gacggggcac cgtgggtgat cccctccgga
    21601 ggcaacaacg cgagtcgggc gtcgaggtcg gtgtgccacc aggcgaggcg acgctcgggc
    21661 aggacggcgg tggcggcggc atacagggag ggcggggcgg ccgacgggtc ggtgacggga
    21721 cgggttccgg cgctggcgcg gaaggcgctc gggacggctc cgtcgggacg accgatgacg
    21781 agcgtctggc ggcctcggga cagcgccgac acggcttcgg cgacgctgac cgtggtggcg
    21841 tcgtcgagga cgaccacgtc gacggcggtg ccgggcgcga ggacctgcgc gacggcatac
    21901 gggctgacgg ccaggcacgg acgcaaggcg ccgatgaccc ggtcggcctc gcggtgcagg
    21961 gccgcaaacg gcaggtgccc atggccgagc cccgcctgcg cgtggaggag gtcggtctgg
    22021 cgggggtgct cgcgagcgcg ctggcgggcg cgacggtcga cgaccgcgcg gacccggccc
    22081 gcgtcgctcg tgcgtccgac ctcgtcgagc ccggcgagtt ggtcggcggc caggcgcagg
    22141 gcgccgccgt cgtggttggc gtagcgcggg tcgcgggcgc ggatctcctg gcccagcgac
    22201 gcccaccaga cgtgctcgac ctcggcgggc acgtgctcgg cggcgacccc gcgcgcggcg
    22261 aagtcatcca tcacctcgcc catccgcgcg gcccgcaact cgtcgacgac cgcagtgacc
    22321 tggggcagga tgtcgagccg gtcgagccgc tcggccagcc gggtcaggcg ggcgcgcagc
    22381 agcgggaacg aggtcgtgag catcggcccg tcgtcgggtc cggggcgcag ccgctcgtcg
    22441 agccaggtga ggtcggcaac gagcccttgg tatgccgcct cgccctcctc caaccgcggg
    22501 gagatctcgg gccggccgcc ggcgccgacg aggtcgtgcc aggcgatccg ttggttgcgg
    22561 gcgcggacca actcggcgtg caggtcggcc ggggggcgac cgggacggag cattcggcgg
    22621 gcctggcggc gcacgcgcga gcgcgaccag ccgccgagct cgacggccga ctcggcgcga
    22681 taggcgcggc tgccggtggc gacgacgtgc tcatcgagcg ggacgtcgaa gatctcgggc
    22741 cggaagacct cgagggtctc gtggacgccg cgcatggtga cgagggcgtg gcgccagtcg
    22801 gcggcggagc gggccgggcg caggctggac tcgacgagga tcgagtcgag atcggcggtc
    22861 gtcgcggcca gatcgccgcc ggtgagccgc gtgacgatgt cgcgggcccg ctcgacctcg
    22921 gcctcggagc ggatggtggc gccgtaccag gggtcgccgc cctcggccga ccaggctccc
    22981 gcggcggctg cggcctgcag gtgctcggcc aactcggtga cccgtgcgcg ggagagctct
    23041 tcgagggcgg cgccgccgat ccgcacccgg gagcccggcg cggggtgacg cgcggcgagg
    23101 gcgcccatcg cctcctggat ttcgtatgcc gtgactcccc acgggcggcg cacctcgtgg
    23161 atcgcggcga catggtcggc gagggtctgc tcggactgcc cgacggtggc cgcccggtcg
    23221 aggcgcccgc gagggtcggc ggcgtcggcg aggtcgcggt catcgagggt cgcgaggcgg
    23281 gcgagtgctt ggcctagctc gctcgtgacg gcgcggcgat cctcaccggc gccggtgagg
    23341 tccaggacga ggtcgtcgag gccgacggag gcgagccggt cctgcagttc gcgcaacgac
    23401 cgacgctggg aggtgacgta gaggacctgc ttgccgtcgt gggcgagggc ggcgacgagg
    23461 gcggcgaccg tttgggtggc accggtgccg gtcggggcgg tgaggacgag ttggcggccg
    23521 gcgcggaccg cctcgatgac cgactcctgg gtggcgtcga ggtcgaggac ccgcagctcc
    23581 agctcggggt cgggggtctc ggcgggctca gggagggcct gccgcagccg ggtgatggcc
    23641 ggctcgtccc cggcgagggc ggcgacgagg tcgacctggg cgagccattg ggcctgaccg
    23701 ctgacgtcgg cgaccatgtc gggcttgccg taggggtagg tcccgagaac caacctcggg
    23761 gtgacggcga agccggggag gtcggcgcag aggcgaccga gggcggcata cacggggtag
    23821 gggtcgaagc cacccgcgga gcccttgagc gtcgtgaggg cggcgagggc ggtggggtcg
    23881 acggcgatgc ccgcgaccga gcgcaggtag ttgacgagca cggggttaac ctcgacggtc
    23941 ggcccaaggt cgaggtcgaa gtcgtgctgg gccgggctgg tggggcgcag ggtgcaggtg
    24001 cgcagcagga ccggggcctc gacgatcgcg cgggcgcgcg gcgggtccca ggtggcgacg
    24061 ccgatggcga cgaacccgac gaccaggccg cgctcgttga ggagctcgga ggccttggcg
    24121 tggatcgccc ggacggtgtc gcgggcctcc tcgaaggcgg cggcctcgcg gactaggtcg
    24181 gacaggctgg tgggacggcc ggcgagcagc atcgagacgc cgccggggtg ggcggtggtg
    24241 agatcgaggt agccgtcgcg gtcaggcccc tcgggcgccc agagcagggt gttgcggccg
    24301 ccgacctcgg acagctcccg ggcccagctg gctatcgtct gcgcaacact caccgggtca
    24361 ggctaaccgg ggcagcgcgg ttggctcggg tgcccgcgcc gagggagcgc cgtttggtgt
    24421 ggttcggggc tgcctgggta tgccgcgtgc cccggttccg tgcgggtccg gcttccgcgg
    24481 gtccggcttc cgcatgcccg gcggctccgc ttggggattg gctcggggct ggggccgcct
    24541 gtcacaatgg cggtcgcggc tgtgcccgtc ccgggacggc tggccgtgcc ccgccctcgt
    24601 agctcagggg atagagcacc gctctcctaa agcgggtgtc ggcagttcga atctgcccgg
    24661 gggcacccaa ccgttcccca ggtctacggc cattttcggc ctcctctcgc tactccgctt
    24721 cgaggcatgc tcccccgctg gggccccatg ggcttcccga gacatcgcac ttcacgatcg
    24781 cccctcgagt cgggcaaccc ggcgagacaa tccggcaatg gcctcgttcg ccaccgccag
    24841 cgacgactca agggatgccg ctcgatcccc gaccgccagc tcgttggcgc ttttcatctt
    24901 gcgctcgatg ccgccacgca ggggtccctg cgggtttggg ccgaactact gccaaacgcc
    24961 gagttggccg gagccgagga gggcatgagc cgccatcagg gcagcctccg ccactctcag
    25021 tgcgtcctcc aggtctcccg cgtcggggcc ggcttcgtcg gagtcggggt attcgtatgc
    25081 gttgcgatta cggcgaattc gaccgaagga gcggaagggc gagccgggcc caccaaactg
    25141 cgcctggacg gcgtcttgga tagcgatgtg accgccgcgg ctggtggcgc ggagcccttg
    25201 gacggcgagc agcgcggcgg cagccttacg cagtgcgtcg tatgccagtt ggtaggcgcc
    25261 ggtcaggtcg ctcagtcgtt cagcagatcg ggctgactcg atgtggcgtg tgccgtccgc
    25321 gaggagtcgc gccgcgaggg ccgcgtccgg agtgacccgt tcaagctctc cgccggccac
    25381 gagggtggac acctcggcac ggcctggccc ccaggtcagg acgcgtcacc tgtctcaggc
    25441 acggagcgga tggggacaag cgggcggctg gttacttcgg cgtggaagcc gtctgtgcgg
    25501 ttgcgccacc acgccagcga ccggatcgtc acattcacct cgcgccccag ccgacggtag
    25561 gcacgctggg cagcatcgtc gagctcgtca cgatcgggac gaccgatcac gagaacatcg
    25621 aggtccgcag gtgcccggcc gggcacaccg gcataccgcg cggcccagga cccgtagaga
    25681 tacagacctt cgatgccccc aacggaagcg aactcctcgg cgatcacctg gggcgggccg
    25741 aacgaccgga gcagcaactc ggtcagcggt tgggtgatcg gcgagggcag tgcgcggacc
    25801 agccgagtgt tcccgacccg ccgtgagaca acaacgccgg catcctcggc ccgggagacc
    25861 tctcgttggg ctgtcgaaac cgaggcgcca acgcggttgg cgagctccgt gagcgaccat
    25921 tcttgttccg ggttcagcag gatgagggcc aggacctccc cttgcagggc agaccgcagc
    25981 aacggcaaga gctgcggagc tggagattgc ataacctgga agatactttg cacgttacgg
    26041 taagtcaacg taggcagcgt gctcaaggcg cgctgggcgg catacctctg cggggaggtg
    26101 gcagcacggc gtgaagtacc gggtcggctt cgctcccgag gccgccgagc aactggtcgc
    26161 gttgcagcgc tacctcaccc cgatcgccac ggcggcttca gagcgtcacg gttgaactca
    26221 acgggatgtc gtcgacgctg gtgccgacca tgacgttgaa ggtccccggc tcgtgagccc
    26281 agccaactcc gttctgccag tgagcaaacg cccgtgccgg cacgctgatc tcgaccgcca
    26341 cggactcgcc cgcgtcagcg cgcaccaccg cgtgacccgc cagccactgc acgggccggt
    26401 caacgctgct ttcgggacgc gccagataga cctggaccac ctgcttgccc cggcgcgcgc
    26461 ccgtgttggt caccgtcacc cccacgcgca cgtcgccgcc gctcgccgca acctcctcca
    26521 ccaccaggtc gcgcaactcc cacgtcgtgt agccgagacc aaaaccaaag gggtatgccg
    26581 cccgcggcac ctctcgcggc tcatccccca gcccatcccc cagccccgcc cgcagccagg
    26641 cgcggtagcc gaggtggatc ccctcggcat actccagctt tccgtccacg ggagtgacat
    26701 cgaggaccgg cacatcggcc atgctcgctg cccaagtcgt cggcaaccgg ccacccggct
    26761 caacccgacc gagcagcacg tcggccagcg cattccccat ctcctgcaca ccgaagtagg
    26821 gcagcacgat cgccgaaacg tcgtcacgcc agggcaattc gaccggtgac ccggagttga
    26881 cgaccaccac ggtgggggtg cccgtcgccg ccaccgccgc gaccaggtcg tcctgtcgac
    26941 cgggcagccg caggtcgctg cggtcgaagc cttcagactc gacctgcgcg ttggtcccga
    27001 cgacgacgat ggccacgtcc gaggcggctg cgacagccac cgcgtccgcg atccgagcct
    27061 ccgggtcggt gtcggacggc gccacgccca gggtgatccc gaacgcgcca tcggtcgtct
    27121 cctgcatgat cgccaggtcg agctcaagga ccacatggac gggccgccca gccgtgacgg
    27181 acacagcaac cggagccacc gccttcggcg ggtcaaggat gttggctccc acgtcggtgc
    27241 tgtcgccgac cgccacatcg tccagcacca gcgccccgtc cacgctgatc tgcccgcggc
    27301 cgatcgacgc gaatcccact gtcacatcac cgttttcggt cggcgtccac cggcacgaca
    27361 actccagcac agccgccgtc aaggtcggtg cgttgcctgc gaagaacacc aacgaggtcg
    27421 cgaaccggtc ctctcgcatc aactcgacac cagcatcgtc gaggaagcgc accaacacgc
    27481 caggcccacc ggtcgccggg ttggtcatct cctccaacgg cagagccgtg atccccatcg
    27541 cggcaaccgc gccgatccga taggtcacct cggcgtccgg cagcgccgcc acgatcccct
    27601 cccacgggct gacgacgcgc tccgggatga ccgtcgccga cccgccgccc tgggtccgcg
    27661 cggccaccgc gttctgaccg atcaccgcga cccgctgcag ccgcgccaca tcgagcggga
    27721 gcaccccatc gttggccaac aacaccgtgc cctcggccgc cgcctccctg gcgaatgcga
    27781 ccccgtcctc gacccgcagc aagcctccaa catcagccgg cccaccggca tacgactcaa
    27841 gcgccccgac ccgctgcgcg agcgtcaaca gccgcgcgac cttccggtcg atcgccgcct
    27901 ccgacacccg cccgtcccgg accgcctcga ccaacgcggc accccacgcc ccctccggcc
    27961 ccggcatcgc caggtcctgc gacgccgacg cggccgcaag gctgcggacc gcggtccagt
    28021 cgctgaccac caccccgtcg aagccccact cggagttcag cggcgtctcc aacaggtcgt
    28081 tctccgacgc cgtcaccccg ttgatcgagt tgtaggacga catcaccacc cacacccgcg
    28141 cctcgacgac cgccttctcg aacgcccgca gatagacctc gcgcaacgtc cgctcgtcca
    28201 cctcgctgct cgcggtgaac ctctccgtct catactcgtt ggccacgtag tgcttcaccg
    28261 tggccccaac gccgttctcc tgcaccccca tcacgtatgc cgcggccaac tccgcggtca
    28321 gccacgggtc ctcgctgaac gcctcgaaat gccggccacc cagaggcgaa cggtgcaggt
    28381 tgatcgtcgg gccgagcacg acatcgacgc ccttgcgccg ggcctcctgc gccgagacgt
    28441 tgccataacg tcgggcgatc tccgggtccc acgacgacgc caacgcagtg gccgacggca
    28501 ggttcaacga cggcgagcgc tcatcccaca gcggtccgcg cactcccgac ggcccatccg
    28561 aaaagaccat ggagcgcaaa ccgattcgct cgatcggcca cgtcgaccag gtgtcccggc
    28621 cggtgagcag ccggaccttc tcctccaggg tcagcaccgc gatgagcgcg gcgtgagtcg
    28681 ccccaaccat ctcgtcctgc gatgtcatcc ccgcgcctct ccctgcctga ctggtcatcg
    28741 aacccgccgc aacgatccct cgtcgaggtg atactccgcg tccgcctgat cagcgcacca
    28801 cgggtcatgc gtcgccagca acacgaccgc ccccgcgtca gcctcggcgc gcaacaacgc
    28861 cacgatcgtc tcgcgggtcg ccccgtccaa ctcgctcgtc ggctcgtccg cgagcagcac
    28921 gctgtgccgc aacgccaagg cccgcgcgac cccgacccgc tgttgctcgc cgccggacaa
    28981 ctcctcgacc aggtggttcc ccgactcggc cagcagcacc gacgcgagcg ccgcgtccgc
    29041 ccgcgcgtgc gcctcccccg cgccgacgcc gtgcatcagc agcggcacga cgaggttctc
    29101 ccgcgccgtc agggccgtca cgagggcgac cccctgcggc acgaggacga tccccgcacg
    29161 cgcggcagca acccgatcgc ctactggcgc agcggcatac atcaccgtcc ctgacgtcgg
    29221 agcgagcgcg ccggcgagga tccacagcaa ggtcgacttg cctgcccccg acggtccggt
    29281 gagcgcggtc atcgtccccg gcgcgagcgc gaacgtgacg tcgtccagca ccggccgccc
    29341 gtcactcaac tcgtagacga cgcccgtcgc caccagcggc cgggcggccc gcatcacgtc
    29401 ggtcggcacg gctcccgctc ctcctgcctg ccagtgggta tgccgtgtac ggtacaggcg
    29461 cgggcgccgc accgaccgtg ccgcacgggg agggcagatg ttgggagcgt tgcgctaccg
    29521 acgagcacag gctgtcgtcg tcgtcgtgct ctcggccctc gtcacggcgt gcctcgtgct
    29581 cgctcccctc tacacccggg cacttgagca gtcgacggtc accaccctcc tgcgcgccgc
    29641 cactccgaca gagtccggca tccgcttggc ctcgttcagt tcggccgagc ccggcatcgc
    29701 gctcgacccc gacgacctgg ccgagctcat cccgagcagc atccgcgccc gctacgggac
    29761 ggcgatcgca gcgacgtcgg tgggcgtccg gcggatgccg ctggggccag agcccggcgg
    29821 tgtgctcctc gcccgggaca acgcctgcgc tcacgtcacc ttcgtcgagg gtcagtgccc
    29881 gagcgccgcc ggcgagatcg ccgtgtccgc cgaccaggcc aaggtctatg aacacgccat
    29941 cgggacagcc gtgttcgtcg gcgaattcga cggcgaacac ggctgtcccg atggcgtgtt
    30001 c
//
LOCUS       DAOMZY010000009        28887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_1287_length_28887_cov_6.240739, whole genome shotgun sequence.
ACCESSION   DAOMZY010000009 DAOMZY010000000
VERSION     DAOMZY010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 28887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28887
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1287_length_28887_cov_6.240739"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="PLK64_01180"
     CDS             complement(<1..1173)
                     /locus_tag="PLK64_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPV78380.1"
                     /translation="MTASPAQSVAAPTTQPGGIPQSYAVPAPLVAALTRRLITGGRPE
                     LRTVVTPMTGQALYELPCSSRADVEVAIDHARGAQRAWSRLPIEHRARIIMRVHDLVL
                     DRQVQLLDLMQLESGKTRLQAFEEIADVALVARHYARAGGSDLASKRAWGAYPVLTRA
                     ETIRQPYGVVGIVSPWNYPFTLPIGDSLPALLAGNAVVLRPDPQTTLTALFGAQMLAD
                     AGLPDGVLQVVSGEGPTVGQAVVDLADYVCYTGSTEVGRAVAQTAARRLVPSSLELGG
                     KNTLYVRSDADLRRAVPGAVRASFGSAGQLCVHAERVVLHESIADAFLADFVPAVAAM
                     RLGPALEYGVDMGTLVSQRQLDRVQAHVADAVAQGAQVLVAGHARPVVSECGGHTSN"
     gene            complement(1176..2297)
                     /locus_tag="PLK64_01185"
     CDS             complement(1176..2297)
                     /locus_tag="PLK64_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HPV78381.1"
                     /translation="MTEIEIGRGKRGRRAYSFDEIAVVPSRRTRDPEDVSVAWQIDAY
                     HFDIPVMGAPMDSVMSPSTAIALGRLGGLPVLDLEGLWTRYDDPEPLLDEIAQLDAAH
                     ATSRMQQIYAEPIRPELITERLRELRDAGVTVAGALSPKHTQAHWRTVLDAGVDLFVI
                     RGTTVSAEHVGTRAEPLNLKRFIYELDVPVIVGGASTYTAALHLMRTGAAGVLVGFGG
                     GAAHTTRRTLGIHAPMASAIADVAAARRDYLDESGGRYVHVIADGGVGTSGDIVRAIA
                     CGSDAVMLGAALARAAEAPGTGFHWGPEAHHQSLPRGERVQVGVRASLAEILFGPGRS
                     ADGTTNIIGALRRAMATTGYSDVKEFQRVEVVVAPHSHA"
     gene            complement(2427..2627)
                     /locus_tag="PLK64_01190"
     CDS             complement(2427..2627)
                     /locus_tag="PLK64_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78382.1"
                     /translation="MVSVAGNVSVPVTLTVVTEFAGLKPRLREEAAPTVLKGLLDMGI
                     ADGPKGVRVRPASPVGGARCGW"
     gene            complement(2741..4261)
                     /gene="guaB"
                     /locus_tag="PLK64_01195"
     CDS             complement(2741..4261)
                     /gene="guaB"
                     /locus_tag="PLK64_01195"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPV78383.1"
                     /translation="MIDSSGSDSAPFAPLGLTYDDVLLLPGETDVVPSEVDTTARLTR
                     EISIRLPLLSSAMDTVTESRMAIAMARQGGLGILHRNLSIVDQAYQVDVVKRTQTGMI
                     TNPITIGQDATLEDLDEICGQYRVSGLPVVDATNHLLGIITNRDLRFTPVAEWGTTLV
                     RDVMTPMPLITAPEGISRDAATLLLRQHKRERLPLIDDEGRLAGLITVKDFVKSEQFP
                     HASKDAQGRLMVGAAIGYFGEAWERAGTLVEAGVDVLVVDTAHGHARLLLEMVRRLKK
                     DPATAHVQVIGGNIATRAGAQALVDAGADAVKVGVGPGSICTTRIVAGVGVPQVTAVH
                     EAAKAARPAGIPVIADGGLQYSGDIAKALVAGADTVMVGSLLAGCEESPGELIFVNGK
                     QFKSYRGMGSLAAMSSRGKKSYSKDRYFQADVTSDDKIIPEGIEGQVLYRGPVGSVVH
                     QLVGGLHQSMFYVGAQTIPQLQERGRFVRITPAGLKESHPHDVQMTVEAPNYTSRS"
     gene            complement(4302..5114)
                     /locus_tag="PLK64_01200"
     CDS             complement(4302..5114)
                     /locus_tag="PLK64_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78384.1"
                     /translation="MTEPLRIDELGADDRLLDALAARTYDGADPIGGLLLSFARACDK
                     PSPTALTRKRRSGRRIVVSTFATAVFLASGASVAAAVSGYEPDRDDAWASSIEQWWST
                     LPGLSGGQGEVVAVPARATSASSSTSTTSLWAHDAPTRVVPPTPTPAVIVVPAETSAD
                     ASASSTESSSSAAPTGEVETPTPPTTPTAPTEPTPTPTPSTPSSASPGSTDGSSHPAG
                     PPAGKSGSTPGQNANPGGPAMAPEPQPGGRPTGGHNVPAAMLEALDLAPAAS"
     gene            complement(5111..5698)
                     /gene="shbA"
                     /locus_tag="PLK64_01205"
     CDS             complement(5111..5698)
                     /gene="shbA"
                     /locus_tag="PLK64_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor ShbA"
                     /protein_id="HPV78385.1"
                     /translation="MEEPGAPLSLRELAIVARSTPGSATEALLGQVRTMALRYARARL
                     GRYGVEDAAQDVAQEVCVAVLTALPTFDDRGLPFEAFVYSIAARKVADAQRRVMRSAA
                     PMANVPEHADEQASPEDLALLRDDAGQVIALLASLPPAQREIIILRVAMGMSTEETAA
                     ALGMTAGAVRVAQHRALTKLRSHYAGIDDGDGERS"
     gene            6010..6300
                     /locus_tag="PLK64_01210"
     CDS             6010..6300
                     /locus_tag="PLK64_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HPV78386.1"
                     /translation="MTEISRLPGPLEHAWTWQQTGSCRDLGPDAFFHPEGERGPARRK
                     RASAAKSVCGSCPVLRQCREHALTVREPYGVWGGLTEEERLAVYAGVLPIPA"
     gene            complement(6424..8043)
                     /gene="groL"
                     /locus_tag="PLK64_01215"
     CDS             complement(6424..8043)
                     /gene="groL"
                     /locus_tag="PLK64_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144865.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPV78387.1"
                     /translation="MAKTLEFDDAARKSLERGVDALANAVKVTLGPKGRNVVIDKKWG
                     APTITNDGVTIAREIELEDPYENLGAQLAKEVATKTNDIAGDGTTTATVLAQAMVKEG
                     LRNVAAGAAPSALKRGMDKAVEALNDRLLANARPVDGTEEIGQVAALSAQDEAIGATI
                     AEAFDKVGKDGVISVEESSSTATELEFTEGMQFDKGYISAYFVTDAERMEAVLEDAYI
                     LINQGKISSIQDLLPVLEKVVQTGKPLVVVAEDVDGEALSTLVVNKIRGTFTVVAVKA
                     PGFGDRRKAMLQDLAILTGGQVISEEVGLKLDQAGLDVLGQARRVVVTKDTTTVIDGQ
                     GGKDDVDGRVAQIKKEIERTDSDWDREKLQERLAKLAGGVCVIKVGAHTEVELKEKKH
                     RIEDAISATRAAIEEGIVAGGGTALIHAATAIDELTLTGDEATGAAIVRKAASEPLRW
                     IAENAGLQGYVVTTKVAELPIGQGLNAATGDYEDLVKAGVIDPVKVTRSALRNAASIA
                     SMILTTDTLVVEKKEEEPAPAAGGHGHGHGH"
     gene            complement(8056..8349)
                     /gene="groES"
                     /locus_tag="PLK64_01220"
     CDS             complement(8056..8349)
                     /gene="groES"
                     /locus_tag="PLK64_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPV78388.1"
                     /translation="MSVSIQPLEDRILVKSLEAETTTASGLVIPDTAKEKPQEGEVLA
                     VGPGRMTDKGVRVAMDIKVGDRVIYSKYGGTEIKHGGQEFLLLSARDVLAIVG"
     gene            complement(8484..9686)
                     /locus_tag="PLK64_01225"
     CDS             complement(8484..9686)
                     /locus_tag="PLK64_01225"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HPV78389.1"
                     /translation="MTAPLEGLLVADFSRVLAGPFATMYLADLGATVVKVERPGSGDD
                     TRAWGPPWTERTSSYFESVNRSKLGLTLDLADPADQAMACELARRADILVENFRPGTL
                     DRMGLGYEQVRDGDPATGRPGNRGIVYASITGYGSGAGASIPGYDFVVQAVGGLMSIT
                     GEPDAPAQKVGVALVDVLTGKDAVIGILAGIAARARSGEGVRVEVDLLSSLLGALVNQ
                     ASGYLATGTPPQRMGNRHPSIAPYETLACADGQLAVACGNDGQFRRLAAAVGLAHLAD
                     DAAYATNSARVAHRDALAIALEGRLRAQPVAHWDGVLRGVGVPAGPVGDIGSAFALAR
                     DLGLEPTYAAHPGHVAQVRNPVRLSGHDPGPGMPPPRLGEHDAVVRGWLSGGDPSLAR
                     LALDLTEC"
     gene            9744..10976
                     /locus_tag="PLK64_01230"
     CDS             9744..10976
                     /locus_tag="PLK64_01230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPV78390.1"
                     /translation="MDPALLARLASGPGRALLDSLEGYAADSALALSTRLRAAGHEPE
                     LVTAVLAQARLRGLAVEKFGAAAGGMLFTADGLEQATRPELASRHASRFVAAGVDHVV
                     DLGCGIGSDARAFAASGLSVEAVEVDPVTAAVARANLAEWPNAHVSEGSAETVDLAKL
                     RGAREAGGRHTGVWVDPGRRVSGVRDSAGRARRVFSLEAISPPWPQVLAWAAQVPATG
                     AKLSPAFPHAATPPGTEAQWTSWRGEVLECAIWWGPLAQVPGRTAAVCRPGASPTIVT
                     EADVRDAPVIGRLDEVGAWFYEADRAVVRAGLSGALPGRELAAGVGYATGSEPIDLPW
                     ARQYAVVEAMPLRVKAVRAWLRARGIGRVTVKKRGASVDPESLRRELTTRAEGHATLV
                     VTSVAGHPVAIVVDPATR"
     gene            complement(10973..11791)
                     /locus_tag="PLK64_01235"
     CDS             complement(10973..11791)
                     /locus_tag="PLK64_01235"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HPV78391.1"
                     /translation="MGRVLVLGSLNVDVVARVDRLPSPGETVSAVAPIQRLAGGKGGN
                     QAVAAAAAGARVVMVGAVGDDEPGRAYTVRLAGRGIFPRLAIAFGVPTGTAYVWVDED
                     GENSIVVAPGANALARAEVADLTPEDVLLCQLEVPVDVVADAVRTAARLGARVVLNAA
                     PYAALPDDVLAVADPLVVNEHEAGLLADSGAAVGSLLVTFGAAGAQWDGERVDGIPVP
                     ADEVLDSTGAGDAFCGALAARLAEGDDRRSALAWAVRAGADAVRREGAQSDPEF"
     gene            complement(11843..12805)
                     /locus_tag="PLK64_01240"
     CDS             complement(11843..12805)
                     /locus_tag="PLK64_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HPV78392.1"
                     /translation="MSRHVVVGAGGIGRATSLALAEAGHEVVLASKSGVGRELPGVKR
                     VAVDATDRRALTALAEGAASLVNAVNPPTYTHWDRDWPPMAAAFLTAAQASGAGLVTI
                     GNLYGYGPVDGPIHPGLPLAATGVKSRVRAQMWADALAAHQAGRVKATEVRASDYFGP
                     DIQPQASVLQRFVFGPAAAGKAVRPFDGSPDAPHSWSYVPDIGAVAAALATSDLGWGR
                     AWHVPSGMPRSLAQAAADAAAFAGHPAPSIRPVAPWVRGLLGTFVPVIRELDETAYQR
                     ERAFVIEAAETESTFGVTATPWQDALAATLTAMGVVPAVASSRV"
     gene            12963..13715
                     /locus_tag="PLK64_01245"
     CDS             12963..13715
                     /locus_tag="PLK64_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPV78393.1"
                     /translation="MTEPASPRPTPGRADGIRARNRAGIEAELRAAARRQLAEVGPAA
                     LSLRAVARDMGMVPSALFRYVSGRDELLTLLIVEAYTSLADAVDEAHDAVDPADLHGR
                     WAALATSMRAWALANPHEWALLFGSPVPTYQAPSEQTTDPGTRVSRLLLAIGRDAALR
                     GVAGSAPFGPATRAADLAAAATANLISEPDVTSSGMSAVTLANGLATWAMLVGAVSSE
                     VFEQFGPQTIANHEAFFTYQVAIGESLLFGRG"
     assembly_gap    13840..13939
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14116..15129
                     /locus_tag="PLK64_01250"
     CDS             14116..15129
                     /locus_tag="PLK64_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPV78394.1"
                     /translation="MTPAQRWGQLIMVGIQPAQAPGGIDATIRASHVGSVIYLGGWSG
                     AGTVRATSDHLQQVAAGPADLFIAADQEGGAVQQLKGKGFTVLPAALDQGKLPPSELT
                     ALGTTLAAELRSAGVNVNLAPVADTVPPSMVQTNAPIGKYRRELGNDPAVVAAAVPVL
                     VTAVQAGQVVATIKHFPGLGRIIGNTDVTAVGITDSQATVDDPYLEPFRAGIAAGTGM
                     VMVSSARYPKLDNANPAMFSRAIVTDLLRTRLGFDGVIVTDDVGAAKAVAAIPVPDRA
                     VRFLAAGGDLVLTASAAQVPSMIAAIKARAASDPAFAAEIEQSVRRVLALKARFGLLR
                     CGG"
     gene            complement(15154..16200)
                     /gene="tsaD"
                     /locus_tag="PLK64_01255"
     CDS             complement(15154..16200)
                     /gene="tsaD"
                     /locus_tag="PLK64_01255"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPV78395.1"
                     /translation="MNDEPLVLGIETSCDETGIGIVRGSTLLVDAVASSVDEHARFGG
                     VVPEVASRAHLEAMVPTLERACRDAGVALRDIDAVAVTAGPGLAGALLVGVAAAKALA
                     VGLGKPIYGVNHLAAHVAVDVLEHGPLPEPTMALLVSGGHSSLLLVPDVTGDVRPLGA
                     TIDDAAGEAFDKVARVLGLPFPGGPHIDRVAREGDRVAIDFPRGLTTGRDLDRYRFDF
                     SFSGLKTAVSRWVQVRQAAGESVPVADVAASFQEAVVDVLTRKAVAACRDQGVDDLLI
                     GGGVAANSRLRALAQERCDRAGIRLRVPRPGLCTDNGAMVAALGAQFVARGRTPSLLD
                     LPADSSMPVTSVQA"
     gene            complement(16197..16658)
                     /gene="rimI"
                     /locus_tag="PLK64_01260"
     CDS             complement(16197..16658)
                     /gene="rimI"
                     /locus_tag="PLK64_01260"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HPV78396.1"
                     /translation="MSAREVTMRELRWSDIPALAQAERELFADDAWTEPTWWAELAGR
                     PRRDYVVAAASDGTIAGYAGVDCAGDTADVMTIAVDTAYRGTGLGDRLLGDLLGRASA
                     AGAQSVLLEVRADNAPALGLYGRHGFEQVSVRRRYYQPGDVDALVLRRRLP"
     gene            complement(16655..17311)
                     /gene="tsaB"
                     /locus_tag="PLK64_01265"
     CDS             complement(16655..17311)
                     /gene="tsaB"
                     /locus_tag="PLK64_01265"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPV78397.1"
                     /translation="MLLLALDTSTSAITVALHDGDRVLAQATTLDARAHGERLAPGIQ
                     QVLAQAGATPTELTHVVCGLGPGPFTGLRVGIVTARVLAFTTGAALHGMCSLDALAAD
                     GPTGEILVATDARRKEVYWARYAVSPGSVRALTPAAVDRPADLPEQVRALPTLGRGPL
                     LYPELFPAPVGILDVSAAALAGLAVRRLAAGAPLSDHEPLYLRRPDAAPHIVTKSALG
                     "
     gene            complement(17321..17839)
                     /gene="tsaE"
                     /locus_tag="PLK64_01270"
     CDS             complement(17321..17839)
                     /gene="tsaE"
                     /locus_tag="PLK64_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPV78398.1"
                     /translation="MSPSSGPLTIRLPDADAARAFGLALATVLRAGDVLVLTGDLGAG
                     KTTLTQGLGEGLGVRGPITSPTFVIARVHPSLVGGPALVHVDAYRLGGAVELDDLDLD
                     ADLDASVVVVEWGHGVAEQLSADRLEVVLQVVGPGETRQATVWATGERWADPTARAAL
                     AALASGAPLADD"
     gene            complement(17829..19025)
                     /locus_tag="PLK64_01275"
     CDS             complement(17829..19025)
                     /locus_tag="PLK64_01275"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPV78399.1"
                     /translation="MARGRPNLVGLGVSLGAAGAAGALGAAGLIADRIRRREDAQLPE
                     YSSLIEAPSSELTVIADDGVTLYVAIDEATGPTKTAAGHPKPTVVLSHGYCLTSECWV
                     LQRRALRRAGYRVVTWDQRGHGRSGMGPKVGYHIDQLGRDLYAVLQAAAPTGDLALIG
                     HSMGGMTIMALAESYPDVIRDRTIAFGAIATSPGSLPLANGGFVAATGKQVLERLGPS
                     VFTGLSKRPELLGTLLKANRDLEEFLVERYSFASPVPRSVVRLTARMLMGTDLAVMSD
                     FVPTFDAYNKIPALAHFAHIETLVFNGTQDVLTPPAHSELIVRGIPGSEHVLIRDAGH
                     VIMLEHPDLLNDQILALLDRAELGRAKHLDPAEKPHVTLHVTPVGKARRVRDGLRNSR
                     KREHVS"
     gene            complement(19025..20194)
                     /gene="alr"
                     /locus_tag="PLK64_01280"
     CDS             complement(19025..20194)
                     /gene="alr"
                     /locus_tag="PLK64_01280"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPV78400.1"
                     /translation="MTSPLPPARPGSQATAYVDLGAVEGNVRALRGHAGPAALMAVVK
                     ADAYGHGLVPVARAAIRGGATWLGTARLSEAITLRDNGIPGRLMAWQAVPGADFAACL
                     EREIDLGVSAPWALDEIVAAARHTGTEARVHLKVDTGLGRAGAYAADFTALLDAAVAA
                     AREGTVRVVGVWSHLACADEPGHPSIRAQQLVFEAAVAEAARAGCELEVRHLANSAAT
                     VTDPSLHYDLVRTGIAIYGLSPVPALGDSAAYGLTPAMTLVARLALVKDVPAGQGVSY
                     GHLYTTTEATRLGLVPLGYADGIPRNASNVGPLAVGGVRHHIAGRVCMDQVVVDLGAG
                     SNARAGDDVVIFGTGGAGEPTAQEWAVSTGTISYEIVTRLGPRVEREYVGVEEVG"
     gene            complement(20240..21706)
                     /locus_tag="PLK64_01285"
     CDS             complement(20240..21706)
                     /locus_tag="PLK64_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPV78401.1"
                     /translation="MIQAYAVADVRAVEAALMERLPEGELMARAANGLAEVVRARLAE
                     RGGHRVVALVGGGNNGGDALYAAALLAQAGFAVGAVCTAYGSVHQGGRDAALAAGVVV
                     TGGDGPEWEALLAEADIVVDGITGIGGRPGLGVEAQGWVGAIPETAYVVAVDLPSGLD
                     PAGVEGTAECVFADETVTFGVAKPCHLLPATEAAAGRLTVVDIGLDFAGCTPVVERLL
                     GADVAAAWPVPTAASDKYSRGVVGVVAGSAAYPGAGVLCVLGALGTGPGMVRYLGPTS
                     VAALVHGHAPEAVTATGRVQAWVLGSGVDPEDTDDEDQLRWIRQALDSDLPCVVDAGA
                     LALLGPRSAPTLITPHAGELARLLTRLAGAGEAEVTREDVLGRPIDHAQAAADALGAV
                     VLLKGATTLVVSPTAAGRAIRSQADAPPWLATAGAGDVLAGILGTLVAAGLDLVNAAS
                     VGAFVHGVAADRANPGGPVRALAVARKVPGVVAAVLTA"
     gene            complement(21703..22059)
                     /locus_tag="PLK64_01290"
     CDS             complement(21703..22059)
                     /locus_tag="PLK64_01290"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HPV78402.1"
                     /translation="MILGVGIDVVDIDRFGASLERTPALRQRLFTESERGLPLRSLAA
                     RFAAKEALAKALGSPVGLSWSDATVLRGEDGRPELDVRGSVLARTKELGIQTLHVSLS
                     HDAGIASAVVVAEGEP"
     gene            complement(22064..23923)
                     /gene="glmS"
                     /locus_tag="PLK64_01295"
     CDS             complement(22064..23923)
                     /gene="glmS"
                     /locus_tag="PLK64_01295"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPV78403.1"
                     /translation="MCGIVGYVGQASDGTALDVVMEGLARLEYRGYDSAGVAVIDGDH
                     VAFRKRSGKLVNLQDAIAAEPLPQTATAIGHTRWATHGGPTDDNAHPHLGGSHGRLAL
                     IHNGIIENFHALKTELLEQGVAFASETDTEVAAHLVSAAYDRTHDLTAAMLEAVARLD
                     GAFTLLAIHADQPGVVVGARRNSPLVVGLGTGANYLGSDVAAFIGHTREALELGQDQI
                     VTITPDSVTVLGFDGTPAEGRRYHVDWDAAAAEKGGYSSFMAKEIDEQPHAVADTLLG
                     RTKENGELVLDELRVSEDALRAVDKIVIVACGTAAYAGMVAKYAIEHWTRIPCEVELA
                     HEFRYRDPVVDGNTLVVSVSQSGETMDTLMAVKHARELGALTVSICNTHGSTIPRESD
                     AVIYTHAGPEIAVASTKAFLAQITACYLLGLYLAQLRGGAFADEARAVMRDLREMPDK
                     IQTVLDRLGRVREIARFMADTRSVLFLGRHVGFPVALEGALKLKEIAYIHAEGFAAGE
                     LKHGPIALIEPGQPVFVIVPGPDAPHDLHAKVVSNIQEIRARGARTLVVAQDGDEAVV
                     PFADEIIRVPATAPLLQPLLTVVPLQVFACELATAKGLDVDQPRNLAKSVTVE"
     gene            23980..24951
                     /gene="coaA"
                     /locus_tag="PLK64_01300"
     CDS             23980..24951
                     /gene="coaA"
                     /locus_tag="PLK64_01300"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015937796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HPV78404.1"
                     /translation="MWRVTQRRESNGSFPTPFVELDRSAWSRLAKNHPLEITADDLTR
                     LRGLDHPLNLGEVQEVYLPLSRLLTFYCEAVEQLHGVTSDFLMERRQRTPFVIGVAGS
                     VAVGKSTTSRILRELLSRWPSTPNVELVTTDGFLLPNAELRRRGLMHRKGFPESYDRR
                     ALLRFLAEVKGGRPEVSVPVYSHLSYDIVPGERIVVRQPDVLIVEGLNVLGAPPVRPM
                     GGTGMAVSDYFDFSVYVDARVSHIERWYVDRFLTLRATAFANPNAYFHRYAALSDEEA
                     VATALTIWETINGPNLVENILPTRQRATLVIVKGEDHSVQKVRLRKL"
     gene            complement(24990..25787)
                     /locus_tag="PLK64_01305"
     CDS             complement(24990..25787)
                     /locus_tag="PLK64_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78405.1"
                     /translation="MPRVERMIVAERYVGRHRRAELALAAATAGSAPKGLAFRPLLHA
                     GASVALATAAVGGYLTAGGAADTNGVTLADPAFASANVAASLSRTLADAPVALTAFDA
                     TAADAEIAQANRERVSRVVSVTTANEIAAQAAAAEAARLDVERQAAAERATRDAQRAS
                     ILANAQRDPKAVAAVLVADRGWSTAQFGCLVKLWTKESNWRWNADNPTSSAYGIPQSL
                     PGSKMASAGADWRTNPVTQIKWGLGYIASRYGSPCSAWSHSVNHNWY"
     gene            complement(25945..27270)
                     /locus_tag="PLK64_01310"
     CDS             complement(25945..27270)
                     /locus_tag="PLK64_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPV78406.1"
                     /translation="MAASRTSANGGAGRKTYVLDTSVLLSDPRAITRFAEHEVVLPVV
                     VVTELEAKRHHPELGYFARTALRMLDDLRVAHGRLDCALPIGDLGGTVRVELNHSNPD
                     VLPAGLRLGDNDTRILAVAKNLAADGNDVTVVSKDLPMRVKASAVGLDAQEYRAELAV
                     DSGWTGMADLDMTVEDMAELYEHGRVEHAAAAELPCHTGLVLHSPRGSGLGRVGADKQ
                     VRLVRGDRDAFGLHGRSAEQRIALDLLLDPDVGIISLGGRAGTGKSALALCAGLEAVL
                     ERRQHRKVVVFRPLYAVGGQELGYLPGTEAEKMGPWGQAVFDTLGALLSTEVVEEILD
                     RGMLEVLPLTHIRGRSLHDAFVIVDEAQSLERNVLLTVLSRVGQNSRVVLTHDVAQRD
                     NLRVGRHDGVAAVIETLKGHPLFAHVTLTRSERSPIAALVTDLLERLDL"
     gene            27542..>28887
                     /locus_tag="PLK64_01315"
     CDS             27542..>28887
                     /locus_tag="PLK64_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HPV78407.1"
                     /translation="MSKSGDGDNASRALPESPVEVDQEQRDQEAAKQAARHLKRLRKR
                     EMAAAALVQAGGDPDPEDFLARRPSIEYHTSTTGPSLMTKALLAGTPGLRTLQHYRRH
                     WLRSDIFAGVAVVAYMIPQCMAYSAIVGVPPEVGLSTALGALIVYAVMGQSRMLSVGP
                     ESTVALIAGVAIAPFANGDPVKATTLGAALSLIVAGWCFVARMLRLGIVAELLSQPLL
                     VGYLAGAAVLMVVGQLGKMTGTKVHGESIVEQVQSFLGVASGTHLFTLWVGIGTFVFL
                     MLIHWVRHRWPATLIAVAVATVVCTVFQLKNQGVAVLGTVPTGLPLPSIPAVSWSEIE
                     ALLAAGVGVAIVAYGDNTLVARGFPAPIDPDEDRAVNQLDPQQELIALGGCHVVVGLL
                     GGFPVSSSGSRTALAVASGARTQMYSVVAAFMLIIVLFVAGPLIANLPQAALAAVV"
BASE COUNT         4254 a        10108 c        10202 g         4223 t
ORIGIN      
        1 gttcgacgtg tggccaccgc attccgaaac aacgggtcgg gcgtggccgg cgacgagcac
       61 ctgggcaccc tgagcgaccg cgtcggcgac gtgggcctgg acccggtcga gttggcgttg
      121 cgacacgagg gtgcccatgt cgacgccgta ttccagcgcc gggcccagcc gcatcgcggc
      181 gaccgccggc acgaagtcgg cgaggaacgc gtcggcgatc gactcgtgca gcaccacgcg
      241 ctcggcgtgc acgcacaact gtccggccga gccgaagctg gcccggacgg cgccggggac
      301 cgcccggcgc aggtcggcat cagaccgcac gtagagggtg ttcttgccgc cgagttccag
      361 cgacgagggc accaaccgac gcgcagcggt ctgggcgacc gcgcggccga cctcggtcga
      421 gccggtgtag cagacatagt cggccaggtc gacgaccgct tgacccaccg tcggtccctc
      481 gcccgacacg acctgcagca caccgtcagg cagcccggcg tcggcgagca tctgggctcc
      541 gaacagggcc gtcaacgtcg tctgcgggtc gggccgaagc accacggcgt tgccggcgag
      601 caaggcgggc aggctgtcgc cgatcggcag cgtgaacggg tagttccacg ggctgacgat
      661 ccccacgacg ccgtagggct ggcggatcgt ttcagccctc gtgaggaccg gataggcccc
      721 ccacgctcgc ttgctggcca gatcgctgcc gccggcccgg gcgtagtgcc gcgccacgag
      781 ggcgacgtcg gcgatctcct cgaaggcctg cagccgagtc ttcccggact ccagttgcat
      841 gaggtcgagc aactgcacct gccgatccag cacgaggtcg tggacccgca tgatgatgcg
      901 agcgcggtgc tcgatcggca gtcgcgacca cgcccgctgc gcaccgcgcg catggtcgat
      961 ggccacctcg acgtcggccc gcgacgagca cggcagctca tagagagcct gcccggtcat
     1021 cggggtcacc acggtgcgca actcggggcg accgccggtg atcaaccgcc gagtgagcgc
     1081 ggccacgagc ggtgccggga cggcatacga ctggggtatg ccgcccggct gggtggtggg
     1141 ggccgcgacg gactgggccg gtgaggcggt catcgtcagg cgtgactgtg aggggcgacg
     1201 acgacctcga cgcgctggaa ctccttgacg tcggagtagc ccgtcgtggc catggcccga
     1261 cggagcgctc cgatgatgtt cgtcgtgccg tcggcgctgc gaccagggcc gaagaggatc
     1321 tcggcgaggc tggcgcggac cccgacttgc acccgctcac cgcgaggcag cgactggtgg
     1381 tgtgcctcgg ggccccagtg gaatccggtg cccggggctt cggcggccct ggccaacgcg
     1441 gcaccgagca tcaccgcgtc ggagccgcag gcgatcgccc ggacgatgtc gcccgacgtg
     1501 cccacgcctc cgtcggcgat gacgtggacg tagcggccgc cggactcgtc gagatagtcg
     1561 cgccgtgcgg cggcgacgtc ggcgatggcc gaggccatgg gagcgtggat gcccagggtg
     1621 cgacgggtgg tgtgcgcggc gcctcccccg aagccgacga gaacgccggc cgcgcccgtc
     1681 cgcatgaggt gcagggcggc ggtgtaggtg gacgcgccgc cgacgatgac ggggacgtcg
     1741 agctcgtaga tgaagcgctt gaggttgagg ggttcggccc gggtgccgac atgctcggcc
     1801 gagacggtcg tcccgcggat gacgaacagg tcgacaccgg cgtcgagcac ggtgcgccag
     1861 tgggcttggg tgtgcttcgg gctgagcgct ccggcgacgg tgacgcctgc gtcgcgcagt
     1921 tcccggagcc gctcggtgat gagctcaggg cgaatcggct cggcgtagat ctgctgcatc
     1981 cgggacgtgg cgtgggccgc gtcgagttgg gcgatctcgt ccaggagtgg ctcggggtcg
     2041 tcgtagcggg tccagagccc ctcgaggtcc aggaccggca gcccgccgag gcgaccgagc
     2101 gcgatggcgg tcgatggcga catcaccgag tccatcggcg cccccatgac ggggatgtcg
     2161 aagtggtaag cgtcgatctg ccacgcgacc gacacgtcct cggggtcgcg ggtgcgccgc
     2221 gagggcacca cggcaatctc gtcgaaggag taggcccggc gaccgcgttt gccacggccg
     2281 atctcgatct cggtcaccca cccaggctac cgggcgggag gtcgcctccc ctgcgcggcg
     2341 gcacacccag gggtatgccg tctcgctcgg ttgcgccgtg acaggtgatc tgtcgactgt
     2401 gtcgcgacag gaagtcccgt tggaactcac catccgcacc gtgcgccgcc aacagggctg
     2461 gccggacgga cccggacacc cttcggcccg tcagcgatgc ccatgtccaa caaacccttc
     2521 agcaccgtcg gggcggcttc ctcacgcaga cgcggcttca acccggcgaa ctcggtcacc
     2581 accgtcaggg tgaccgggac gctgacgttc ccggcgacag agaccaccga ccgacccccc
     2641 gccccaaacc ccctctccca caaccgaaca catcacggtc aggtagcgat ctcaggcggg
     2701 ttcgggaccg ggtggtgccc cgggagcaca ccctgacggg ctacgagcgc gaggtgtagt
     2761 tgggagcctc gacggtcatc tgcacgtcgt gcgggtggct ctccttgagc cctgccgggg
     2821 tgatccggac gaaccggccg cgctcctgca actgcgggat ggtctgggcg ccgacataga
     2881 acatcgactg gtgcaggcca ccgacgagtt ggtggacgac cgagccgacc gggccgcgat
     2941 agaggacctg cccctcgatg ccctcgggga tgatcttgtc gtcgctggtg acgtcggcct
     3001 ggaagtagcg gtccttggag taggacttct tgccgcggct ggacatcgcc gcgagcgacc
     3061 ccataccccg gtaggacttg aactgcttgc cgttgacgaa gatgagctca cccgggctct
     3121 cctcgcaccc agcgagcagc gaccccacca tgacggtgtc ggccccggcg acgagggcct
     3181 tggcgatgtc gccggaatac tgcaggccac cgtcggcgat gacggggatc cccgccgggc
     3241 gagccgcctt ggccgcctcg tggactgcag tcacctgggg gacgccgact ccggcgacga
     3301 tgcgggtcgt gcagatcgag cctggcccga cccccacctt gaccgcgtcg gcaccggcgt
     3361 caacgagggc ctgggcgccc gctcgggtag cgatgttgcc gccgatgacc tggacgtgcg
     3421 cggtcgccgg gtccttcttg agccgacgga ccatctccag caggagccgg gcgtgaccgt
     3481 gggccgtgtc gacgacgagc acgtcgacgc cggcctcgac gagcgtgcca gcgcgctccc
     3541 aggcctcgcc gaagtacccg atcgccgcgc cgaccatgag tcgaccctgg gcgtctttgg
     3601 aggcatgcgg gaactgctcg gacttgacga agtctttgac ggtgataagc ccggcaaggc
     3661 gcccctcgtc gtcgatcagc ggcagtcgct cgcgcttgtg ctggcgcagc aggagggtcg
     3721 ccgcgtcgcg ggagatgcct tcgggcgcgg tgatgagcgg catgggcgtc atgacgtcgc
     3781 ggaccagggt cgtgccccat tcggccaccg gagtgaagcg caggtcgcgg ttggtgatga
     3841 tgccgagcag gtggttggtg gcgtcgacga ccggcagccc cgacacgcgg tactgcccgc
     3901 agatctcgtc gaggtcttcg agcgtggcgt cctgcccgat ggtgatcggg ttggtgatca
     3961 tcccggtctg ggtgcgcttg acgacatcga cctggtaggc ctggtcaacg atcgagaggt
     4021 tgcggtgcag gatgccgagg ccgccctgac gagccatggc aatggccatc cgtgactcgg
     4081 tgacagtgtc catcgctgag gacaacagcg ggagccggat cgagatctcg cgggttagcc
     4141 gcgcggtggt gtcgacctcg ctgggcacga catcggtctc gcccggcaac agcagaacgt
     4201 cgtcataggt gagccccagg ggagcgaacg gcgcgctgtc agaaccggac gagtcgatca
     4261 tcgcccgagt ctaccggggc cgaggcgccc cgcccctcgg ctcaacttgc ggccggagcg
     4321 aggtccaagg cctcgagcat cgcggcaggg acattgtggc cgcccgtcgg tcggccaccg
     4381 ggttggggct ccggagccat ggccggaccg cccgggttgg cgttctgccc gggggtgctg
     4441 cccgacttac ccgcgggggg cccggcgggg tgcgacgaac cgtcggtgga gccggggctc
     4501 gccgacgagg gggtgctcgg cgttggcgtt ggcgtcggct cggttggggc cgttggggtc
     4561 gttgggggcg tcggtgtctc cacctcgccc gtcggggccg ccgacgacga ggactcggtg
     4621 gacgaggcgg aggcgtcggc ggatgtctca gcgggcacga cgatcacggc gggcgttggg
     4681 gtgggcggca cgacccgggt cggggcgtca tgagcccaca gagaggtggt cgacgttgag
     4741 gaacttgcgg aggtcgcccg agccggcacc gcgaccacct caccctggcc ccctgatagc
     4801 ccggggaggg tgctccacca ctgctcgatg ctggacgccc aggcgtcgtc gcgatcgggc
     4861 tcgtagccgc tgaccgcagc ggccacgctc gcacctgagg caaggaacac ggccgtggcg
     4921 aacgtgctga cgacgatccg tcggccggaa cgccgcttgc gcgtcagcgc cgtcggcgat
     4981 ggcttgtcgc aggcccgggc gaaggacagc aacagcccgc cgatcgggtc ggcgccgtca
     5041 tacgtgcgtg ccgccaaagc gtcaaggagc cggtcatcgg ccccgagctc gtcgatgcga
     5101 agtggttcgg tcatgaccgt tccccgtccc catcgtcgat accggcatag tgcgatctca
     5161 gcttggtgag agcccggtgc tgggcgactc ggaccgcacc ggccgtcatg cccagggcgg
     5221 ccgcggtctc ctcggtcgac atgcccatgg ccacgcggag gatgatgatc tcgcgctggg
     5281 caggagggag actggccagc agggctatga cctggccagc gtcgtcgcgc aacagggcca
     5341 ggtcctcggg actggcttgt tcgtcggcgt gctcgggcac gttcgccatc ggggccgccg
     5401 aacgcatgac ccggcgttgc gcgtcggcca ccttgcgagc ggcgatggag tagacaaacg
     5461 cctcgaaggg cagaccgcgg tcgtcaaagg tcggcagagc tgtgagaaca gccacgcaca
     5521 cctcctgggc gacatcctgc gcggcatcct cgacgccata ccgacccagc cgagccctgg
     5581 catagcgcag ggccatcgtc cgaacctgtc ccaggagggc ctcagtggcc gaaccgggcg
     5641 tcgagcgtgc cacgatggcg agttcgcgca gcgagagcgg cgcgcctggc tcttctatcg
     5701 cagaaagcgg ttgcggcgtt acggcgaccg gaacgtcgcg ccgacgtgca gcacccgtag
     5761 accacaccac tgtttccccg attgatcgac gtcaacggct caccgtcgat gagcgtacgc
     5821 gacaccaagg tcgcatgagt agtttacggc tgtacttctt tcgccgaacc cttacccatt
     5881 ctttaacaag ccttgactca caccgtgacc acctccggcg tgcttcgggt tagcgttccg
     5941 aacaccggac ggcatacggg cggagtcgtt cgggagtcgt cacgacgtcg cgcacagtgg
     6001 ggagttacgg tgacggagat ttcgcgattg ccagggccgc tggagcacgc ctggacctgg
     6061 cagcagaccg gctcctgccg tgacttgggc ccggacgcgt tcttccaccc agagggtgaa
     6121 cgaggtccgg cccgtcgcaa gcgtgcctca gcggccaagt cggtgtgcgg atcgtgcccg
     6181 gttcttcggc agtgccgcga gcacgccctc accgtgcgtg agccctacgg cgtgtggggc
     6241 ggcctgaccg aggaggaacg gctcgccgtc tacgcaggag tcctgccgat ccccgcttga
     6301 tgcgggacgt ccccatgtga ccactcccgg accgagtggt gaaacgccga acgggccgcg
     6361 ctccctcggg gggatcgcgg cccgttcggc gcggctcacg cgccagcagg tatgccgtgg
     6421 ggctcagtgc ccgtggccgt gcccgtgccc accagcggcg ggagcgggct cctcttcctt
     6481 cttctccacg accagggtgt cggtcgtgag gatcatcgag gcgatcgacg cggcgttgcg
     6541 cagcgcggac cgggtgacct tgaccgggtc gatgacgccg gccttgacca ggtcctcgta
     6601 gtcgccggtg gcggcgttga ggccctgccc aatcggcagc tcggccacct tggtcgtcac
     6661 gacgtagccc tggagcccgg cgttctcggc gatccaccgc agcggctcgg aggcggcctt
     6721 gcggacgatc gccgcacccg tcgcctcgtc accggtgagg gtcagctcgt cgatggccgt
     6781 cgcagcgtgg atgagagcgg tgccgcctcc ggcgacgatg ccctcctcga tcgccgcgcg
     6841 ggtcgccgag atggcgtcct cgatgcggtg cttcttttct ttgagctcaa cctcggtgtg
     6901 ggcaccgacc ttgatgacgc agaccccgcc ggccagcttg gccagacgct cctgcagctt
     6961 ctcgcggtcc cagtcggagt cggtgcgctc gatctccttc ttgatctgag cgacccggcc
     7021 gtcgacgtcg tccttgccac cctgaccgtc gatgacggtc gtggtgtcct tggtgacgac
     7081 gacccggcgg gcctgcccga gcacgtcgag accggcctgg tcgagcttga gcccgacctc
     7141 ctcggagatg acctgaccgc cagtgaggat ggccaggtcc tgcagcatcg ccttgcggcg
     7201 gtcgccgaac ccgggcgcct tcaccgcgac gaccgtgaag gtgccgcgga tcttgttgac
     7261 gacgagcgtg gagagagcct ccccatcgac gtcttcggcc acgacgacca acggcttgcc
     7321 ggtctggacg accttctcga gcaccgggag caggtcctgg atcgaggaga tcttgccctg
     7381 gttgatgagg atgtaggcgt cctcgaggac ggcctccatg cgctcggcgt cggtgacgaa
     7441 gtaggccgag atgtagccct tgtcgaactg catgccctcg gtgaactcca gctcggtcgc
     7501 cgtcgacgag gactcctcga ccgagatgac gccgtccttg ccgaccttgt cgaacgcttc
     7561 ggcgatggtg gcgccgatgg cctcgtcctg ggccgagaga gcagcgacct ggccgatctc
     7621 ctcggtgccg tcgaccgggc gggcgttggc cagcagacgg tcgttgagcg cctcgacggc
     7681 cttgtccatg ccgcgcttga gcgccgacgg ggcagccccg gcagcgacgt tgcgcagacc
     7741 ctccttgacc atcgcctggg cgaggaccgt cgcggtcgtc gtcccgtctc cggcgatgtc
     7801 gttggtcttg gtggcgacct ccttggccag ctgagcgccg aggttctcgt aggggtcctc
     7861 aagctcgatc tcgcgcgcga tggtcacccc gtcgttggtg atcgtggggg cgccccactt
     7921 cttgtcgatg acgacgttgc ggcccttcgg gccaagcgtc accttgacgg cgttggcgag
     7981 tgcgtcgacg ccccgttcaa gggacttgcg ggcggcgtcg tcgaactcca gcgtttttgc
     8041 cacgagctac ccgcctcagc cgacgatggc gaggacgtcg cgggccgaca gcaggaggaa
     8101 ctcctggccg ccgtgcttga tctcggtgcc gccgtacttg ctgtagatga cccggtcgcc
     8161 gaccttgatg tccatggcga cgcggacgcc cttgtcagtc atccgcccgg ggccgacggc
     8221 gagaacctcg ccctcctgcg gcttttcctt ggcggtgtcc gggatgacca gcccggatgc
     8281 ggtggtggtc tcggcctcga gggacttgac caggatgcgg tcctcgagcg gctggatgga
     8341 aaccgacacg tcggtgtcct ttcggaagtc gatggatgtc atgactgtgg tcgctcggcc
     8401 ggcctcgccg tcgcggggct cgacgctggc cgtgcgatca gcactctcca ggggagagtg
     8461 ctaacgcaaa tctaggtatc tcattagcac tcggtcaagt cgagtgccag ccgagccagg
     8521 ctgggatctc cgccggagag ccagccacgc accacggcgt cgtgctcacc gaggcgcgga
     8581 ggcggcatac ctgggcctgg gtcgtggccc gagaggcgga ccgggttgcg cacctgggcc
     8641 acgtgaccgg ggtgggcggc atacgtcggc tcgaggccga ggtcccgcgc gagggcgaag
     8701 gcggacccga tgtccccgac cggcccggcc ggcactccca ctccccgcag cacgccgtcc
     8761 cagtgggcga ccggctgcgc gcgcagccgc ccctccaggg cgatcgcgag cgcgtcccgg
     8821 tgggcgacgc gggcgctgtt cgtcgcgtat gccgcgtcgt cggcgaggtg cgcgagcccc
     8881 accgcggccg cgagccggcg gaactgcccg tcgttgccgc aggcgacggc gagctgccca
     8941 tcggcgcagg ccagcgtttc gtaaggggcg atggacgggt gtcggttgcc catccgttgc
     9001 ggcggcgtgc cggtggcgag atagccggag gcctggttga cgagcgcccc gagcaggctg
     9061 gagagcaggt cgacctcgac gcggacgccc tcgccggagc gcgcgcgggc ggcgatcccg
     9121 gcgaggatcc cgatgaccgc gtccttgccg gtgaggacgt ccacgagggc cacgccgacc
     9181 ttctgagccg gggcgtcggg ctcgccggtg atgctcatca gcccgccgac ggcctggacg
     9241 acgaagtcgt agcccgggat cgacgcccct gcccccgagc catagccggt gatcgaggcg
     9301 tagacgatgc cccggttgcc cggccgaccg gtggccgggt cgccgtcgcg gacctgctcg
     9361 taaccgagcc ccatccgatc gagggtgccg gggcggaagt tctcgacgag gatgtccgcg
     9421 cggcgggcga gctcgcacgc catcgcctgg tcagcgggat cggcgaggtc gagggtgagc
     9481 ccgagtttgg agcggttgac cgactcgaag taggaactcg tgcgctcggt ccacggcggc
     9541 ccccaggccc gcgtgtcgtc gccagacccg ggccgctcga ccttgacgac ggtcgcgccc
     9601 aggtcggcga ggtacatcgt cgcgaagggg ccggccagga cgcgactgaa gtcggcgacc
     9661 agcaagccct ccagaggggc cgtcatgacg tcagcctagg tccggcccgg tgtccgtccg
     9721 tcgtggtccg ggaagatggg gggatggacc ccgccctgct cgcccgcctc gcctccggcc
     9781 cgggtcgcgc gttgctcgac tcgctcgagg ggtatgccgc ggactcggcg ctcgcgctct
     9841 cgacccggct gcgggcggcc gggcacgagc cggagctggt gaccgcggtg ctggcccagg
     9901 cccggttgcg agggctggcc gtcgagaagt ttggagctgc tgcaggcggg atgctgttca
     9961 cggccgatgg gttggagcag gccacccggc cggagttggc gtcgcggcat gcgtcgaggt
    10021 tcgtcgcggc gggggtggat cacgtcgttg acctgggatg cgggatcggt tcggacgcaa
    10081 gggctttcgc ggcttcgggt ttgtcagtgg aggccgtgga ggtcgacccg gtgacggccg
    10141 ccgtggcccg cgcgaacctc gccgagtggc cgaacgccca cgtgtcggag gggtccgccg
    10201 agacggtcga cctcgcgaaa ctgaggggcg cgcgggaggc tggcggacgg cataccggcg
    10261 tctgggtcga cccggggcgg cgggtgagcg gcgtgcgcga ctcggcgggc cgggcccgac
    10321 gagtgttctc gctggaggcc atctcgccgc cgtggccgca ggtgctcgcc tgggccgcgc
    10381 aggtgcccgc gaccggggcg aagctgtcgc cggccttccc gcacgcggcg accccgcccg
    10441 gcaccgaggc gcagtggacg tcgtggcggg gcgaggtgtt ggagtgcgcg atctggtggg
    10501 ggccactcgc ccaggtgccc ggccggaccg ccgcggtatg ccgccccggc gcctccccca
    10561 cgatcgtcac cgaggcagac gtccgcgatg cgccggtcat cggcaggctc gacgaggtgg
    10621 gcgcctggtt ctacgaagcg gaccgggccg tcgtgcgcgc ggggctgtca ggggccctgc
    10681 ctgggcggga gctggctgcg ggcgtgggct atgcgacggg ttccgaaccg atcgacctgc
    10741 cgtgggcccg acagtatgcc gtcgtcgagg cgatgccgtt gcgggtcaag gcggttcggg
    10801 cctggctgcg ggcgcgaggg atcggtcggg tcaccgtgaa gaagcgtggg gcttcggttg
    10861 acccagagtc gctgcgccgc gagttgacga cccgcgccga aggccacgcg acgttggtcg
    10921 tcacctccgt tgccggccac ccagtagcga tcgtcgtcga ccccgccacc cgctagaact
    10981 ccggatccga ttgggcgcct tcgcgacgca ccgcatcggc cccggctcgc acggcccacg
    11041 cgagcgccga ccggcggtca tcgccctcgg ccaggcgcgc cgccaaagcc ccacagaacg
    11101 cgtcgccggc accggtcgag tcgagcacct catcggcagg cacgggtatg ccgtccaccc
    11161 gctccccgtc ccactgcgcc cctgccgccc cgaaggtcac cagcagcgat ccgacggccg
    11221 ctcccgagtc ggccagcagc ccggcctcgt gctcgttgac gaccaaagga tcggcaacgg
    11281 cgaggacatc gtccgggagg gcggcatacg gagcggcgtt gaggaccacg cgggcaccga
    11341 gccgggcggc cgtgcggacc gcatcggcga cgacgtcgac cggcacctcg agctggcaga
    11401 gcaggacgtc ctccggggtg aggtcggcga cctcggcccg cgcgagggcg ttggctccag
    11461 gggcgacgac gatggagttc tcgccgtcct cgtccaccca gacataggcg gttccggtcg
    11521 ggacaccgaa ggcgatcgcc aggcgcggga agatgccgcg cccggccagc cgcacggtgt
    11581 aggcccggcc cggctcgtcg tctccgaccg cgccgaccat gacgacgcgc gcgccggcag
    11641 cggctgcggc caccgcctgg ttgccgccct tgccgccggc caggcgctgg atcggcgcca
    11701 cagccgacac ggtctccccc gggctgggca accggtcgac cctcgccacc acatcgacgt
    11761 tgagcgaccc gagcacaagc acccggccca tcagcgacca cctccgacga tccgcttcac
    11821 gtccgcagcg tatgccgcac gatcacactc gcgacgacgc aactgccggg acgacgccca
    11881 tcgcggtgag cgtcgccgcc aaagcgtcct gccagggggt ggccgtcaca ccgaaggtcg
    11941 actccgtctc cgccgcctcg atgacgaacg cccgctcgcg ctggtatgcc gtctcgtcca
    12001 gttcccggat caccggcacg aacgtgccga gcagtccgcg aacccagggc gcaaccggtc
    12061 ggatcgaagg ggccgggtga ccggcgaagg ctgccgcgtc ggcggccgcc tgggcgaggc
    12121 tgcgcggcat acctgagggg acgtgccatg cgcggcccca accgaggtcg ctggtggcca
    12181 aggccgcagc caccgcgccg atgtcgggga cgtagctcca cgagtgcggc gcgtccggcg
    12241 atccgtcgaa gggccgcacg gcctttccgg cggcggcggg accgaagacg aaacgctgca
    12301 ggacgcttgc ctggggttgg atgtcggggc cgaagtagtc cgacgcccga acctcggtgg
    12361 ccttcacccg gccggcttgg tgcgcggcga gggcgtcagc ccacatctgg gcccgcaccc
    12421 ggctcttgac gccggtcgcg gcgagcggca gcccggggtg gatgggcccg tcgaccggac
    12481 cgtagccata gaggttgccg atggtgacca ggccagcacc ggacgcctgc gccgcggtga
    12541 ggaaggcggc cgccatcggg ggccagtccc ggtcccagtg ggtgtaggtg ggcgggttga
    12601 ccgcgttgac caggctggcc gcgccttcgg cgagagccgt gagggcccgg cgatcggtgg
    12661 cgtccacggc gacccgcttg accccgggca gttcccggcc aacgcccgac ttcgaggcga
    12721 gaaccacctc atgaccggcc tcggcgagtg ccagggacgt ggcccggccg atgcctccgg
    12781 ctccgacgac gacgtgacgg gacatgtgaa gctccttctg gttggggctt ccgacggcct
    12841 caagccgccg gtaagtcctg agttgcgatc aacgttctct gattggatca caagtcacta
    12901 ctcaatgtca agaacatcat tcactacacg gatcggcgct ctcgttggcc tacactcgga
    12961 ccgtgaccga acccgcgagc ccccgcccca cccccggtcg tgccgacggc atccgggccc
    13021 gcaaccgggc cggcatcgag gccgagctgc gagccgccgc ccgacgccaa ctggccgagg
    13081 tcggcccggc ggccctgtcc ttgcgcgcgg tcgcccgcga catgggcatg gtcccctcgg
    13141 cgttgttccg ttacgtctcg ggccgcgacg agttgctcac cttgctcatc gtcgaggcct
    13201 acacctccct cgctgacgcc gtcgacgagg cccatgacgc cgttgacccg gccgacctgc
    13261 acggccgatg ggccgcactg gccacgtcga tgcgggcctg ggcgctggcc aacccgcacg
    13321 agtgggccct cctgttcggc tcgccggtgc cgacctacca ggctccgtcg gagcagacca
    13381 ccgatccggg cacccgggtg tcgcggctcc tcctggcgat cggccgcgac gccgcgctgc
    13441 gcggtgtcgc cggttcagcc cccttcggcc ccgccacgcg agccgccgac ctcgccgccg
    13501 ccgcgaccgc gaacctcatc agcgagcccg acgtcacgtc ctccggcatg tccgccgtca
    13561 ccctggccaa cggtctggcc acctgggcga tgctcgtcgg cgccgtgagt tccgaggtct
    13621 tcgagcagtt cgggccgcag accatcgcca accacgaagc gttcttcacc taccaggtgg
    13681 cgatcgggga gtccctcctc ttcggtcgcg ggtagcctcg ccactcatgc ggtccctctc
    13741 caacaccagg tatgccgcgg gcctggctct cgcggccctg ctcgccgccg cctgcaccgc
    13801 aggttcgacg acaccgaccg gttcgaccgg ttcgaccggn nnnnnnnnnn nnnnnnnnnn
    13861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13921 nnnnnnnnnn nnnnnnnnnc gaccggttcg accggttcga ccggggcagc atcgacgggg
    13981 ggcagtgccg gtgggaccac cgcgagcggc gcgacgggca cccagcccgg cgggacggca
    14041 tacccgacga gcggtggcac cacgagcccc accgcaacgg ccggggcgtc ctgcgctgcc
    14101 gaggtcgcga gcggcatgac cccggcgcaa cgctgggggc agctgatcat ggtggggatc
    14161 caaccggcgc aggcccctgg cggcatcgac gcgacgatcc gcgcctcgca cgtggggtcg
    14221 gtgatctatc tcggtggctg gtccggggcc ggcacggtgc gggcgacctc cgatcacctg
    14281 cagcaggtgg cggccggccc tgccgacctg ttcatcgccg ccgaccagga gggcggagcg
    14341 gtccaacaac tcaagggcaa gggcttcacc gtcctgccgg cggcgcttga ccaaggcaaa
    14401 ctgccgccga gcgagttgac ggcgctcggc accaccctgg cggcggagtt gcgcagcgcg
    14461 ggcgtcaacg tcaacctcgc accggtcgcc gacaccgtgc cgccgtcgat ggtccagacc
    14521 aatgcgccga tcgggaaata ccgtcgtgag ttggggaacg acccggccgt cgtggccgcc
    14581 gccgtgccgg tgctggtcac cgcggtgcag gcgggccagg tcgtcgcgac gatcaagcac
    14641 ttccccggtc tgggccggat catcggcaac accgacgtca ccgccgtggg gatcaccgac
    14701 tcgcaggcga cggtggatga cccttacctg gagcctttcc gagccgggat cgctgccggc
    14761 accggcatgg tcatggtgtc ctcggctcgc tacccgaagc tcgacaacgc caacccggca
    14821 atgttctccc gggcgatcgt caccgacttg ctgcgcacga ggctgggctt cgacggcgtc
    14881 atcgtcaccg atgacgtcgg agccgccaag gccgtcgccg cgatcccggt gcccgatcgg
    14941 gcggtgcgat tcctcgctgc gggtggcgat ctcgtgctca ccgccagcgc cgcgcaagtg
    15001 ccgagcatga tcgctgccat caaggctcgg gccgccagcg accccgcgtt cgccgctgag
    15061 atcgagcagt cggtccgtcg agtcctggcc ctcaaggcca ggttcggcct attgcgctgc
    15121 ggcggttagc cacccgggcc gagcggcgcc ggctcaggcc tggaccgagg tcaccggcat
    15181 cgacgaatcg gccgggaggt cgagcagcga cggcgtccgg ccccgcgcga cgaactgcgc
    15241 gccgagcgct gccaccatgg cgccgttgtc ggtgcacagc cccggccgag gcacccgcag
    15301 ccggatcccg gcccggtcgc accgctcctg cgccagcgcc cgcagccgtg agttggccgc
    15361 caccccaccg ccgatgagta ggtcatcgac cccttggtcc cggcaggccg cgacggcctt
    15421 gcgggtgagc acgtcgacca cggcctcctg gaagctggcc gcgacgtcgg ccacgggcac
    15481 cgactccccc gccgcctgac gcacctgcac ccagcgtgag acggccgtct tgaggcccga
    15541 gaaggagaag tcgaagcgat agcggtcgag gtcgcgccct gtcgtcaacc cacgcgggaa
    15601 gtcgatcgcc acccggtcac cctcgcgggc gacccggtcg atgtgcgggc ctccggggaa
    15661 cggcagcccc aacacccgag ccaccttgtc gaaggcctcc ccggccgcgt catcgatggt
    15721 cgccccgagc ggccggacat cgccggtgac gtccggcacg agcagcagcg acgaatgccc
    15781 accggacacc agcagcgcca tcgtcggctc cggcaggggt ccatgctcaa ggacatccac
    15841 ggcgacgtgg gcggccaggt ggttgacccc atagatcggc ttgcccagcc cgacggccag
    15901 cgccttcgcc gcggcaacgc cgacgagcaa ggccccggcc aggcccgggc ctgcagtcac
    15961 agcgacagca tcgatgtcgc gcagggccac tcccgcatcg cggcaggcgc gctcgagcgt
    16021 gggaaccatg gcctcaaggt gagcccgcga ggcgacctcg gggacgaccc cgccaaagcg
    16081 ggcatgctcg tcgaccgaac tggccacggc gtcgaccagc agggtgctcc cccggacgat
    16141 cccgatcccg gtctcgtcgc aggacgtctc gatccccagg acgagcggct cgtcgttcac
    16201 ggcagcctcc gacgcaggac aagggcgtcg acatcgcccg gttggtagta gcgccgccgc
    16261 accgacacct gctcgaagcc gtgccgtcca taaagcccga gcgccggcgc gttgtcggca
    16321 cgcacctcca gcagcacgga ctgcgctcca gcggccgagg cgcgtcccaa caggtcaccc
    16381 aggagtcggt cccccagacc cgtcccccgg tatgccgtgt cgaccgcgat cgtcatgacg
    16441 tcggcggtgt ctccggcgca gtccaccccc gcgtagccgg cgatcgtgcc gtccgacgct
    16501 gccgcgacga cgtagtcgcg tcgaggtcgc cccgccaact cggcccacca cgtcggttcg
    16561 gtccaggcat cgtcggcgaa caactcgcgc tcagcctggg ccaacgcggg gatgtcgctc
    16621 cagcgcaact cgcgcatcgt cacttcacga gcactcatcc gagggccgac ttcgtgacga
    16681 tgtgcggggc tgcgtcgggg cggcgcagat agaggggctc gtgatcggac aggggcgcac
    16741 cggcggcgag ccggcgcacg gccaggccgg ccagcgccgc tgccgacacg tcgaggatcc
    16801 cgacgggagc ggggaacaac tcgggataga gcagcggccc tcggccgagc gtcggaagcg
    16861 cccgcacctg ctcaggcagg tctgcgggac ggtcgacagc cgcgggggtg agggcgcgca
    16921 cggaacccgg cgagacggca taccgcgccc agtagacctc tttgcgcctg gcgtcggtcg
    16981 cgacgaggat ctcgcctgtg ggtccgtcgg cggcgagcgc gtcgagcgag cacatgccgt
    17041 gcagtgccgc gcccgtggtg aaggcgagca cgcgggcggt gacgatgccg acccgcagcc
    17101 cggtgaacgg cccggggccg agcccgcaga ccacgtgggt cagttcagtc ggcgtggccc
    17161 cggcctgcgc gagcacctgc tgtatgccgg gtgcgagccg ttcgccgtgg gcgcgggcat
    17221 cgagggtggt cgcctgagcc agcactcggt cgccgtcgtg cagcgcgacg gtgatggcgc
    17281 tggtcgaggt gtcgagagcg agcaggagca ccggtcaagg ctagtcgtcg gcgagtgggg
    17341 cacctgacgc gagcgcggcg agggcggccc gagcggtcgg gtcggcccag cgctcaccgg
    17401 tcgcccacac ggttgcctgc ctcgtctcgc ccggcccgac gacctgcagg acgacctcga
    17461 gtcggtcggc gctcaactgc tcagccaccc cgtgacccca ttcgacgacg acaaccgagg
    17521 cgtcgagatc ggcgtcgaga tcgaggtcgt ccaactcgac ggcgccgccg agccgatagg
    17581 catcgacgtg gaccagcgcc gggcctccga ccagcgacgg gtgcacccgc gcgatgacga
    17641 aggtcggcga agtgatcggc cctcgcacgc ccagaccctc accgaggcct tgggtgagcg
    17701 tggtcttccc tgctccgagg tcgccggtga gcacgagcac gtcaccggct cgaaggacgg
    17761 tcgccagcgc cagcccaaac gctcgcgccg cgtcggcatc cgggagccgg atcgtgagcg
    17821 gtcccgagct aggagacatg ctctcgcttg cggctgttgc gcagcccgtc gcgcacccgg
    17881 cgcgccttgc cgaccggcgt gacgtggagc gtgacgtgcg gcttctcggc cgggtcgagg
    17941 tgcttggcgc ggcccaactc ggcccggtcc agcaacgcga ggatctgatc gttgagcagg
    18001 tcggggtgtt cgagcatgat gacgtgcccg gcgtcgcgga tgaggacgtg ttcggagccg
    18061 gggatacccc tgacgatcaa ctcgctgtgg gccggcgggg tgagcacgtc ctgggtgccg
    18121 ttgaagacga gggtctcgat gtgcgcgaag tgcgccaacg cggggatctt gttgtaggcg
    18181 tcgaaggtcg ggacgaagtc agacatgacg gcaaggtcgg tgcccatgag catccgcgcc
    18241 gtgagccgga cgacgctgcg cggcaccggc gaggcgaagg agtagcgctc gacgaggaac
    18301 tcctccaggt cgcggttggc cttcagcagg gtgccgagca actcggggcg cttggacagc
    18361 ccagtgaaga cgcttgggcc gagccgttcg agcacctgct tgccggtggc ggcgacaaag
    18421 ccgccgttgg ccaggggcag gctgccgggt gaggtggcga tggcaccgaa ggcgatggtg
    18481 cggtcgcgga tcacgtcggg gtagctctcg gccagcgcca tgatcgtcat gccgcccatc
    18541 gagtggccga tcagcgcgag gtcgccggtg ggagcggccg cctggagcac ggcatacagg
    18601 tccctgccga gttggtcgat gtggtagccg accttcgggc ccatgccgga gcggccgtgt
    18661 ccgcgctggt cccaggtgac cacgcgatag ccggcccggc gcagcgcgcg acgctgcagc
    18721 acccaacact cgctggtgag gcagtagcca tgcgagagaa cgaccgtcgg cttcgggtgc
    18781 ccggccgcgg tcttcgtcgg gccggtggcc tcgtcgatgg cgacgtagag ggtcaccccg
    18841 tcgtcggcga tcaccgtgag ttcgctggac ggtgcctcga tgaggctgga gtactccggc
    18901 agttgggcgt cctcgcgccg gcggatccgg tcggcgatga gcccggcagc acccagggca
    18961 ccagcagcac cggcggcgcc aaggctgaca cccaggccga cgaggttggg gcgacctctc
    19021 gccactagcc gacctcctcc acaccgacgt attcccgctc cacccgcggt cccaacctcg
    19081 tcacgatctc atatgagatc gtcccggtcg acaccgccca ctcctgcgcg gtgggctccc
    19141 ccgcaccccc cgtgccgaag atcaccacat catccccggc gcgggcgttc gacccggctc
    19201 cgaggtcgac gacgacctgg tccatgcaga cccgtccggc gatgtggtgg cgcactccgc
    19261 cgacggccag cgggccgaca ttgcttgcgt tgcgcgggat cccgtcggcg taacccagcg
    19321 ggacgagacc cagccgggtt gcctcggtcg tcgtgtagag gtggccgtag gagaccccct
    19381 gaccggcggg cacgtccttg acgagggcca gtcgcgctac gagggtcatc gccggcgtca
    19441 gcccgtatgc cgcggagtca ccgagcgcgg gcaccggcga caggccgtag atcgcaatcc
    19501 cggtgcgcac gaggtcatag tgcaagctcg gatcggtcac tgtcgcagcc gaattggcta
    19561 ggtgtcggac ctcgagctcg cagccggccc gcgctgcctc ggccaccgcg gcctcgaaca
    19621 ccagctgttg ggcccggatc gacgggtgcc caggctcgtc ggcgcaggcc aggtgcgacc
    19681 acaccccgac gacccgcacc gtcccctccc gggccgcggc cacggctgcg tcgagcaggg
    19741 cggtgaagtc cgcggcatac gccccggccc gtcccagccc cgtgtccacc ttgaggtgca
    19801 cgcgggcctc ggtcccggta tgccgcgccg ccgccacgat ctcgtccaac gcccacggcg
    19861 cggagacccc gaggtcgatc tcgcgctcca ggcaggccgc gaagtcggcc cccggaaccg
    19921 cctgccaggc catcaaccga cctgggatcc cgttgtcccg caacgtgatc gcctcggaca
    19981 accgggctgt ccccagccag gtggcaccgc cgcgaatggc ggcccgagcc accgggacga
    20041 gcccgtgacc ataggcgtcg gccttgacga ccgccatgag cgccgccggc cccgcatgcc
    20101 cgcgcagggc ccgcacgttg ccctcgacgg cacccaggtc gacgtaggcc gtcgcctggc
    20161 tcccggggcg ggctgggggc agtggggaag tcatcgcggg acagtgtgcc agggcacgcg
    20221 gggtcgtcgg tgcgaggagt taggcggtga gcacggcggc gacgacaccg ggaaccttgc
    20281 gagccaccgc gagcgcacgc accgggccgc ccgggttggc tcgatcggcg gccaccccat
    20341 ggacgaacgc ccccacggac gccgcattca cgagatcgag tccggcggcc accagggtgc
    20401 cgaggatgcc ggccaacacg tcgcccgcgc cggccgtggc cagccacggc ggagcgtcgg
    20461 cctggctgcg gatcgccctc ccggcggcgg tgggagacac gacgagggtt gtggcgccct
    20521 tgagcaggac gacggcgccc aaggcatcgg cagcggcctg agcgtggtcg atgggacggc
    20581 ccaagacgtc ctcgcgggtg acctcggcct cccccgcccc ggccagccgg gtgagcaggc
    20641 gcgccaactc gccggcgtga ggggtgatga gcgtgggcgc cgagcgcggg ccgagcaggg
    20701 cgagcgcccc ggcatcgacg acgcacggca ggtcgctgtc gagggcctgg cggatccacc
    20761 gcaactggtc ctcgtcgtcg gtgtcctcgg ggtcgacgcc cgagccgagc acccaggcct
    20821 ggacccgacc ggtggcggtc accgcctcgg gggcgtggcc atgcacgagg gcggcgaccg
    20881 acgtcggccc gaggtagcgc accatccccg gcccggtgcc gagcgcgccc aggacgcaga
    20941 ggacacccgc acccgggtag gccgcggaac cggcgacgac gccgaccaca ccccgcgagt
    21001 atttgtccga tgcggccgtc ggcaccggcc aggccgccgc cacgtcggca ccaagcaggc
    21061 gctcgacgac cggcgtgcaa ccggcgaagt ccagcccaat gtcgaccacg gtgagccggc
    21121 ccgcggcagc ctcggtcgcg ggcagcagat ggcagggttt ggcgaccccg aaggtcaccg
    21181 tctcgtcggc gaacacgcac tcggcggtgc cttccacgcc ggccgggtcg aggccgctgg
    21241 gcaggtcgac cgcaacgacg taggccgtct ccgggatcgc cccgacccag ccttgggcct
    21301 cgacgcccag gcccggccgc ccgccgatcc cggtgatccc gtcgacgacg atgtcggcct
    21361 cggccaacag ggcctcccac tcagggccgt ccccgccggt caccaccaca cccgccgcca
    21421 gcgccgcgtc gcgcccgccc tggtggacgc tcccgtatgc cgtgcacacg gcacccacgg
    21481 cgaaccccgc ctgggcaagg agcgccgcgg catacagggc atccccgccg ttgttgccgc
    21541 cacccacgag agcgacgacc cggtgtccac cacgttcggc gagccgggcc cggacgacct
    21601 cggcgagccc gttcgcggct cgggccatga gctcaccctc gggcaggcgc tccatcaggg
    21661 ccgcttcgac ggcgcgaacg tcggccacgg catacgcctg gatcacggct ctccttcggc
    21721 gacgacgacg gcggaggcga tccccgcgtc gtgggacagc gacacgtgca gcgtctggat
    21781 gcccaactcc ttggtccggg ccaggacgct cccgcgcaca tccaactccg ggcggccgtc
    21841 ctcaccccgc aacacggtgg cgtcggacca cgacaggccg accggcgagc cgagcgcctt
    21901 ggccaacgcc tccttggcgg cgaagcgggc ggccagcgat cgcaacggca acccccgctc
    21961 gctctcggtg aacagccgct ggcgcaaggc cggtgtgcgc tccagcgagg cgccgaaccg
    22021 gtcgatgtcg acgacgtcga tgcctacgcc gaggatcacc gaatcactcc accgtcacgg
    22081 acttggcgag gttacgcggc tggtcgacgt ccagcccctt ggccgtcgcc aactcgcagg
    22141 caaagacctg gaggggaacg acggtgagca acggctgcag cagcggcgcc gtggccggga
    22201 cccggatgat ctcgtcggcg aacggcacga ccgcctcgtc gccgtcctgg gcgacgacca
    22261 acgtgcgggc gccacgggcc cggatctcct ggatgttcga gacgaccttg gcatgcaaat
    22321 cgtgcggggc atccggtccg gggacgatga cgaataccgg ttggccaggc tcgatgaggg
    22381 cgatcggccc gtgcttgagc tcgcccgccg cgaaaccctc cgcgtggatg taggcgatct
    22441 ccttgagctt gagagcgcct tcgagggcca ctgggaagcc gacgtggcga ccgaggaaca
    22501 gcaccgatcg ggtgtcggcc atgaaccggg cgatctcgcg gacccgaccc agtcggtcga
    22561 gcacggtctg gatcttgtcg ggcatctcgc gcaggtcgcg catgacggca cgcgcctcgt
    22621 cggcgaacgc acctccgcgc agttgggcga ggtagaggcc cagcaggtag caggcggtga
    22681 tctgcgcgag gaaggccttg gtcgaggcga ccgcgatctc cggcccggcg tgggtgtaga
    22741 tcaccgcgtc ggactcacgc gggatggtcg agccgtgggt gttgcagatc gagacggtga
    22801 gggcccccag ctcgcgggcg tgtttgacgg ccatgagggt gtccatcgtc tcgccggact
    22861 ggctcaccga cacgacgagc gtgttgccgt ccacgaccgg gtcgcgatag cggaactcgt
    22921 gggccagctc gacctcgcac gggatccggg tccagtgctc gatggcatat ttcgcgacca
    22981 tcccggcata ggcggccgtg ccgcacgcaa cgatgacgat cttgtcgacg gcgcgcaggg
    23041 cgtcctcgga gacgcgcagt tcgtcgagga ccaactcgcc gttctccttg gtccggccca
    23101 acagggtgtc ggcgacggcg tgcggctgct cgtcgatctc cttggccatg aacgacgagt
    23161 agccgccctt ctcggctgcg gcggcgtccc agtcgacgtg atagcggcga ccctcggccg
    23221 gggttccgtc gaacccgagg accgtcaccg agtcgggggt gatcgtcacg atctggtcct
    23281 ggccgagctc aagcgcctca cgggtgtgcc cgatgaaggc cgcgacgtcg gagccgaggt
    23341 agttggctcc cgtcccgagg ccgaccacca gggggctgtt gcgccgggca cccaccacga
    23401 caccgggctg gtcggcgtga atcgccaaca gggtgaaggc gccgtccagg cgggcgaccg
    23461 cctcgagcat ggcggccgtg aggtcgtgcg tgcggtcgta ggccgccgag accagatggg
    23521 cagcaacctc agtgtcggtc tcggaggcga acgcaacgcc ctgctcgagc aactcggtct
    23581 tgagcgcgtg gaagttctcg atgatcccgt tgtggatgag ggccagccgg ccgtgggacc
    23641 cgccgaggtg ggggtgggcg ttgtcgtcgg tggggccacc gtgagtggcc caacgggtat
    23701 ggccgatggc cgtcgccgtc tgcggcaacg gctcggcggc gatggcgtcc tgaaggttga
    23761 cgagcttccc cgaacgcttg cggaaggcga cgtggtctcc gtcgatgacg gccacaccgg
    23821 cggagtcgta gccccggtac tccaaccggg ccaacccctc catgacgacg tccaaggccg
    23881 tcccgtccga cgcttgacca acgtaaccca cgattccaca catggccacc agcgtagttg
    23941 cccccacctg cggccctgtc ccgcccggca tacgcaagaa tgtggcgcgt gacccagcgc
    24001 cgtgagagca acgggagttt tcccacaccc ttcgtggaac tggaccggtc ggcgtggtct
    24061 cggctggcca agaaccaccc gctcgagatc acggcggacg acctcacccg gctgcgcggc
    24121 ctcgaccacc cgctgaacct gggcgaggtc caagaggtct acctgccgct ctcacggctc
    24181 ctgaccttct attgcgaggc cgtcgagcag ttgcacggcg tgaccagcga cttcctcatg
    24241 gagcgccgcc agcgcacgcc cttcgtcatc ggcgttgccg gatcggtggc cgtcgggaaa
    24301 tcaacgacct cgcgcatcct gcgggaactg ctgtcgcgct ggccgtccac ccccaacgtc
    24361 gagctcgtca cgaccgacgg tttcctcctg ccgaacgccg agcttcgtcg gcgaggactc
    24421 atgcaccgca agggttttcc cgagtcttac gaccgccgag cgttgttgcg gttcctcgca
    24481 gaggtcaagg gcggacggcc cgaggtgtcg gtgccggtct actcacacct gtcctacgac
    24541 atcgtcccgg gtgagcggat cgtcgtgcgt cagcccgacg tgctcatcgt cgaggggctc
    24601 aacgtcctcg gcgcgccgcc cgttcgcccg atgggcggca ccgggatggc agtgagcgac
    24661 tacttcgact tctcggtcta tgtcgacgct cgggtgtcac acattgaacg ctggtacgtc
    24721 gaccggttcc tcaccctgcg cgcgaccgcc ttcgccaacc ccaacgccta cttccaccgg
    24781 tatgccgcgt tgtccgatga ggaggccgtg gcgacggcgc tgacgatctg ggagacgatc
    24841 aacggcccga acctcgtcga gaacatcctc ccgacccgcc agcgggccac gctggtcatc
    24901 gtcaagggcg aggatcactc ggtgcagaag gtccgactgc gcaaactctg atcggtcagt
    24961 gcctgatctg gctgcgctcg acccggcggt cagtaccagt tgtggttgac cgagtggctc
    25021 cacgcgctgc acggcgagcc gtagcggctg gcgatgtaac ccagccccca cttgatctgg
    25081 gtgaccgggt tggtgcgcca gtcggcaccc gcggacgcca tcttcgaccc cggcagagac
    25141 tgcgggatgc cgtaggcgct cgacgtcggg ttgtcggcgt tccagcgcca gttgctctcc
    25201 ttggtccaca acttgaccaa gcagccgaac tgcgcggtgc tccagccacg gtcggccacc
    25261 agcacggcgg cgaccgcctt gggatcgcgc tgggcgttgg ccaggatgct ggcccgttgg
    25321 gcgtcacgtg tggcccgctc ggcggcggcc tgccgttcga cgtccagtcg ggcggcctca
    25381 gcggcggccg cttgcgcggc gatctcgttg gcggtcgtca cgctcacgac ccggctgacc
    25441 cgctcgcggt tggcctgggc gatctcggcg tcggcagcag tcgcgtcgaa cgcggtgagg
    25501 gcgacggggg catcggcgag ggtccggctc agggacgccg cgacattcgc tgatgcgaaa
    25561 gccgggtcgg cgagggtaac gccgttggtg tccgctgcgc caccggccgt gaggtaaccg
    25621 ccgacggcgg ccgtggccaa cgcgaccgag gccccggcgt gcagcagcgg ccggaacgcg
    25681 aggcccttgg gtgccgatcc cgcggtcgcc gcagcgagcg cgagttcggc tcggcggtgc
    25741 cggccgacat aacgctccgc cacgatcatc ctttcgacgc gcggcatcgt tggtcgatgt
    25801 cgcatctggc cgtgctctcc cacatctcta gacggaacgc acgacggatt ccgtcacggc
    25861 gttaccaagt cgagatcttc gtcatagagt gccagagatc agccgaacgg acaaatctcc
    25921 accccggcat cgagagcggc agggtcagag gtccaggcgt tcgagcaggt cggtcaccag
    25981 agcagcgatc gggctgcgct cgctgcgggt caacgtcaca tgggcgaaga gcgggtggcc
    26041 cttgagggtc tcgatgacgg ccgccacccc gtcatggcgc ccgacccgca ggttgtcgcg
    26101 ctgggcgacg tcgtgcgtga ggacgacccg ggagttctgc ccgacccggg agagcaccgt
    26161 cagtaggacg ttgcgctcca acgactgggc ctcgtcgacg atgacgaacg cgtcgtgcag
    26221 cgagcggccg cggatgtggg tgagcggcag gacttccagc atcccccggt cgaggatctc
    26281 ctccaccact tccgtggaga gcaacgcgcc gagggtgtcg aagacggcct gcccccacgg
    26341 acccatcttc tcggcctcgg tgcccggcag gtagcccagt tcctggccgc cgacggcata
    26401 caacggccgg aagacgacca ccttgcggtg ctggcgccgt tcgaggacgg cctcgagccc
    26461 cgcgcacagg gcgagtgcgg acttgccggt ccccgcccgg ccgccgagag agatgatccc
    26521 gacgtcgggg tcgagcaaga ggtccagggc aatgcgttgc tcggccgacc ggccgtgcag
    26581 cccgaaggcg tcgcggtccc cacggaccag gcgcacctgc ttgtcggcgc cgacccgacc
    26641 caggccactg ccgcgcggcg agtgcagcac cagcccggtg tggcacggca actccgcagc
    26701 cgccgcgtgc tcgactcgac cgtgctcgta gagctcagcc atgtcttcga ccgtcatgtc
    26761 gaggtcggcc atccccgtcc agccggagtc gacggcgagc tcagcccgat actcctgcgc
    26821 gtccagtccc accgccgagg ccttgacccg catcggcagg tccttgctga cgacggtgac
    26881 gtcgttgccg tcggcggcga ggttcttggc caccgcgagg atccgggtgt cgttgtcgcc
    26941 cagccgaagt cccgcgggca ggacgtcagg gttcgagtgg ttgagctcga cgcgcaccgt
    27001 gccgccgagg tcgccgatcg gcagggcgca atccaaccga ccgtgcgcca cccgcaagtc
    27061 atcgagcatc cgcagcgccg tccgggcgaa gtagcccaac tccgggtggt ggcgtttggc
    27121 ctccaactcg gtgacgacga cgaccgggag caccacctcg tgttcggcga acctcgttat
    27181 cgctcgcgga tcggacaaca ggaccgacgt gtccagaacg taggtctttc gacccgcacc
    27241 accgttcgcg gacgtccgag aagcagccac gcacccctcc ttggcgatct gcacgtgctc
    27301 cgacggtaag cgcccaccgc gccgccgcgc gggatccggc ccggcgcgtc gcgcacccct
    27361 gagcgaagcg agtcgcgggg tgcgccgttg cggcggcgac gcggcatacc cagccggtat
    27421 gccgtcgcgc tgggcaaggg ggtgcgcgcg gtcgttggtt cccctagatt gggcgcgact
    27481 cttccggccg gtcatcccat gttcaccagc cggccatctg acaacgtgtg ggggtgatcc
    27541 catgtccaaa tctggagatg gtgacaacgc gagcagagca ctgccggaat cccccgtcga
    27601 ggtggaccag gagcaacgcg atcaggaagc cgccaagcag gcagcgcgac acctcaagcg
    27661 actgcgcaag cgcgagatgg cggctgcggc cctggtgcag gcgggaggcg acccggatcc
    27721 cgaggacttc ctcgcccgcc gtccctccat cgagtaccac acgtcgacca cgggcccgag
    27781 cctgatgacc aaggccctgc tcgccgggac ccccggcctg cggaccctgc agcactatcg
    27841 ccggcactgg ttgcgctcgg acatcttcgc gggcgtggcc gtcgtcgcct acatgattcc
    27901 ccagtgcatg gcctactccg ccatcgtggg ggtgcccccg gaggtcggcc tctcgaccgc
    27961 gctcggcgcc ctcatcgtgt atgccgtgat gggccagtca cggatgcttt ccgtgggccc
    28021 ggagtcgacc gtcgccctca tcgccggcgt ggcgatcgcg ccgttcgcca acggtgaccc
    28081 ggtgaaggcc accaccttgg gagcggccct ctcgctcatc gtcgcgggct ggtgcttcgt
    28141 cgctcggatg ctgcgcctgg gcatcgtcgc cgagttgctc tcccagccgc tgctcgtggg
    28201 ctatctcgcg ggcgcggccg tcctcatggt cgtcggccaa ctcggcaaga tgaccggcac
    28261 gaaggtccac ggcgagagca tcgtcgagca ggtccaatcc ttcctcggag tggccagcgg
    28321 aacgcacctg ttcaccctct gggtcgggat cgggacgttc gtcttcctca tgctcatcca
    28381 ctgggtgagg catcgctggc cagccacgct catcgcggtg gctgtagcga ccgtcgtgtg
    28441 caccgtcttc caactcaaga accagggcgt cgcggtgctg ggcacggttc ccaccggtct
    28501 gcctctcccg tccatcccgg cggtgtcctg gtcagagatc gaggctctgt tggcagccgg
    28561 tgtcggagtg gcgatcgtcg cctacggcga caacaccctg gtggcccgcg gcttccccgc
    28621 ccccatcgac cccgacgagg accgcgccgt caaccaactc gatccccagc aagaactgat
    28681 cgcgctgggc ggctgccacg tcgttgtggg cctgctcggt ggtttccccg tctcgtcctc
    28741 gggctcccgc acagcgctcg cggtggcgtc cggcgcacgc acccagatgt attcggtggt
    28801 cgcggcgttc atgctgatca tcgtcctgtt cgtggccgga ccgttgatcg ccaaccttcc
    28861 acaggctgcc ctggccgccg tggtctt
//
LOCUS       DAOMZY010000010        24553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_1713_length_24553_cov_6.049882, whole genome shotgun sequence.
ACCESSION   DAOMZY010000010 DAOMZY010000000
VERSION     DAOMZY010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 24553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24553
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1713_length_24553_cov_6.049882"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="PLK64_01320"
     CDS             <1..753
                     /locus_tag="PLK64_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78408.1"
                     /translation="DNADNADDAADMALAPTIKRTLLSDSSQPIDVDDRPAQSPPGPA
                     ANQESPTVSGSSRKPDHRKRVAAVVIAGAAVLTAMGTLAATTRPVSAPGVVAAPTSTG
                     KGIDMVGQGPIERLIVKPTPESLHVSVELSRVLDANSELWVDIQFARTAGNCPDRIIV
                     FSPKQRQATLGTATCQGEVVTYSVATTLSSTLEPGSVNSAALSWAALDGPRGGILIAR
                     VRLVTEGQTVSWLPTNQSYATASTGGGVAIPK"
     gene            complement(846..2066)
                     /locus_tag="PLK64_01325"
     CDS             complement(846..2066)
                     /locus_tag="PLK64_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015938545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPV78409.1"
                     /translation="MTTDGDSGPTRTLLTGRLVTGGTTTHDGAVAIEDGTIAYAGPLA
                     ELPPGWSAVAPDPRVPTGATLVPGLVDSHLHGGAGGEFGVDAEAARTAVAEHRAHGST
                     TLIGSLVSAGASDLITATQTLSALVASGDLAGIHLEGPFIAAARKGAHDPDALRDGDP
                     GLVDALAEAAAAVGAPGAIRHMTFAPDRAGADLLPAALHRHGIRVNVGHTEADATLTF
                     DVLGRAAATAGAPSLVTHIFNAMPPIRGRSPGTAAGAIRAALRGDAVLEVVADGVHLS
                     PDTVRMLFEVVGPGSINLISDAMAATGLPDGNYTLGGLPVVVADGTARLTSSGAIAGS
                     VAHVADCLRWAVQTAGIDLADAVLAATGTPAKAFGLDVGHLVTGGVADVLVLDADLRP
                     ALVVAGGQASPARR"
     gene            complement(2063..2818)
                     /locus_tag="PLK64_01330"
     CDS             complement(2063..2818)
                     /locus_tag="PLK64_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPV78410.1"
                     /translation="MTAVTLPDQILEGPVPKHRQLRDVLARLAVPGEAIPSERDLTAT
                     YGVSRATVRKAIDTLVYEGLLQRTHGLGTFAIRPRLGTQLHLASFTQDMHRRGMVPST
                     RLVSIRREVPPDPIARALGLGPLDRAWSLRRLRLADGQAIALEDAWYPVSLLPGLDQH
                     DLESSLYALFQNQYGLTIDGAEQTLWGEAATGDVARLIACPEHTPLLVFRRVASAGDR
                     PVEYVLSWYRGDRYEVSMSLGSEAAASRAMVPS"
     gene            complement(2941..3426)
                     /locus_tag="PLK64_01335"
     CDS             complement(2941..3426)
                     /locus_tag="PLK64_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HPV78411.1"
                     /translation="MTAILSPCSGRVIALDEVPDPVFAGRIVGPGVAIDPDRSAATVV
                     APISGRLVKLHPHAFVIAGADVAVLVHLGIDTVQLGGAGFTLHATERTDIAAGDPIVT
                     WDPADIEAGGRSPVVPIILLDVPGETPVDAVCGPDAVIGEHLLTWPADSDPDGATQRR
                     E"
     gene            complement(3423..3695)
                     /locus_tag="PLK64_01340"
     CDS             complement(3423..3695)
                     /locus_tag="PLK64_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018770722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose/sucrose transporter subunit IIB"
                     /protein_id="HPV78412.1"
                     /translation="MHAEPHSDPTKGTQMSKAARILSGLGGADNIVEIEACITRLRTE
                     VEDGGLVDQAALKAAGAHGVIVTGNIVQVVVGPEADNLAEDIQDLM"
     gene            complement(3718..3921)
                     /locus_tag="PLK64_01345"
     CDS             complement(3718..3921)
                     /locus_tag="PLK64_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78413.1"
                     /translation="MTATAMAREIAEQPEAVRRTLAAHLSRTDEIAALAAGRRVLFVS
                     RPSRVAQGHPDRIRPLTDPIDPH"
     gene            4156..5397
                     /locus_tag="PLK64_01350"
     CDS             4156..5397
                     /locus_tag="PLK64_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010524051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HPV78414.1"
                     /translation="MNAQAAARQTSGKRFDLAPLQKFGRSLMLPIAALPAAALLLRIG
                     QPDLLGKDGLGWTQVAAVLGAAGDAIFANLPLLFAVGVAIGMAKKADGSTALAGVVGY
                     LVFKGVADAISPFVLGAAAAGEKQKLINYGVLGGIVMGITAAKLWERYHRISLPPYLA
                     FFGGRRFVPIVTALAAIILAVLLGVIYPAFNSGLTALGNWVVANTIIGGLVYGTLNRL
                     LIPLGLHHILNSVPWFVFGEYTAADGQVYHGDIARFLHGDPAAGPFMTGFFPIMMFAL
                     PAAAFAIWRAAKPSQRKAVGGIMLSGALTSFLTGVTEPLEFSFMFVAWPLYVLHSVLT
                     GTSLAVANALGIHDGFGFSAGLFDFALNWNIATKPALLIVLGLIYAVVYYLLFTWVIR
                     KWNLRTPGREDDDSTTADTSA"
     assembly_gap    5534..5633
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5634..8354
                     /locus_tag="PLK64_01355"
     CDS             <5634..8354
                     /locus_tag="PLK64_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPV78415.1"
                     /translation="DGTAVALDVTVFRPGGLGQHSEPWSAVLLAHGFGGSKADLVERG
                     RDLASRGYLVLGYSARGFGASGGRIHLNDPAYEIADARALVSLLAARSDVLLDAPGDP
                     HVGVVGASYGGALAIMLGATDQRVDSVVGVVAWNDLADAFFPQFAVVGPRSPLGPAQV
                     DRSPEPGPLKALWASRFFGSALASAGSPGAGVGAGAHPSSGGGAGAVCGRFDPTVCRL
                     FLEATVTGRPSPELLTVLRAHSPRPLLAGLRAPTMLVQGMADSLFGIEQAAATARAIA
                     VQVPRLAIRWIDGGHDGLSSTAAADEQALRDWLADTLRGTTLLAGAGQFSYAAPIPRR
                     RTVAPLFTTPDSPPTATWTAVPLAPLVGASGGGTSDPQRIVTPPGGQPASVTAIPSLG
                     GLGVGAAAYQLAALPGQSAAFDSPPFAQQTAIVGTPRLTLTVTSTGPETVLFLSLWQV
                     TAGQATLPRRLVAPVRVLTTPGQPTSVDVALAPATWTVEAGSSLRVLVTSTDSAYAAP
                     REARVDLVAVAGGELRLPHVDGDLLAAESDLDTESVGVATAIALLLAAFGVLAWRERR
                     QRRLLPDRADLADVPLAVEHLVKTYADGHRAVGDVSWRAERGQVVGLLGPNGAGKTTT
                     LRMAMGLITPDSGAVYLGGRAVRPGAPALRGVGALVEGPGFLPHLTGRANLHAYWAAT
                     GRPIEEARLDEALDVAALGGAVDRPVRSYSHGMRQRLGIAQAMLGLPDLLVLDEPTNG
                     LDPPQIAAMRPILQRYAAAGRTVVVSSHLLAEVEQTCSHVVVMHAGRVVTAGPVADLI
                     DSSDTTVVHLDPAATAETIAAVADRLLSVAGILEVEIVEDEGDSRLVVTAGMPRPDVV
                     RALTEVGADVVGLSSRKHLEEVFLRVIAAAQTAGEPTGSVTERLRQVRAR"
     gene            8351..9268
                     /locus_tag="PLK64_01360"
     CDS             8351..9268
                     /locus_tag="PLK64_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV78416.1"
                     /translation="MTTDPVAKQPTTTVPTATEPRATEPSATEPSGRHTRGALSFGAE
                     VRRQLGRRRTLWSFVIMLALPVILIGAFALGRTSGPPGGSRLSDLAQQGSANFAIFAL
                     AAASDFLLVVLAALFAGDAVPAEASWSTLRYLLIAPVPRARLLASKWAVAIVSLGVAI
                     VVYLGWGLLVGGLAYGWAPFTNPAGGVLGWGELLPRLAIATAYLFVTLLQVAGIAFVL
                     GVRTDAPLGAVGVAVLVAIVSSILGQIDSLGDLRNGLPLHYIRAWQDLLTPQVDWTAM
                     GHGVLWSVLYTLLTVGLAFAVFRRKDVLS"
     gene            9453..10430
                     /locus_tag="PLK64_01365"
     CDS             9453..10430
                     /locus_tag="PLK64_01365"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPV78417.1"
                     /translation="MLDPKAAGEIARAFGLGANARLEGPVAHGRKGEVWRLVTDEGRY
                     AVKALFGTVFRERAERDAAYQDVVRDTGVPMPAVLRTLSGNVLADVGGPPPNATVRVY
                     EWVDILEPRRDLDPREIGRLLARVHNVEAPAAGTVDPWYREPVGPQVWGELVEALRAE
                     DAPFATDLAALVPQLVALEQTFEVPADVRTCHCDLWADNVRATRDGGIVLLDWESAGP
                     ESPSHELGMVVFEYGLGDPARMLDLYAAYLIAGGPGRISRAGDLTMLGATSEHLAEEG
                     CRRWLSATDEGDRELAAAWVEWLLHEPITPATVADVLDAVAGLGGSRGA"
     gene            complement(10575..11828)
                     /locus_tag="PLK64_01370"
     CDS             complement(10575..11828)
                     /locus_tag="PLK64_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV78418.1"
                     /translation="MSVTPRAPRTPRTPPSAVPAVTSLALEQDVFERLRGVVPRVAAE
                     AVETIMREVPSYARPFSDAMGARIRTAVELALVNFLELARVGSGTDPAEPIAPSTAAA
                     YELGRGEARSGRSMDALQAAYRVGARVCWRGLSREAVATGVPAATLGAFAELVFAYID
                     ALSSASISGHADELATAGRARERYRDRLAALLVGGGEEADLVAAARRADWHPPRSLTA
                     VLLPEGQLRAVLGLLDARTLAMSTEPAGVDDEDAGVLLVPDATGRDRERLVRQLEGRG
                     AIIGPERPWLQVHRSFQRVLRAREFLPQPNSRGTLDTDDLLAELLLSADPEALADLRA
                     RALAPLAAVRPTVRLRLEETLRSWLLHHGRRELIAAHLFVHPQTVRYRMAQLRELFGD
                     RLDDPEEVLRLTMALALQPTRPPTP"
     gene            11924..13018
                     /locus_tag="PLK64_01375"
     CDS             11924..13018
                     /locus_tag="PLK64_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HPV78419.1"
                     /translation="MSATVTSPRGRSLRQRAWRAAEMLTGALMPADTLDVINPLRPGA
                     DPLAGRIERIHLETADAATVFIKPGADWSGHIPGQYLRFGIDIGGVRQWRTYSLTSRL
                     DRPDGLLTITTKAIPDGLVSNHVVRDLRPGTLVHIGQAEGDFVLPTARPDKVLFLTGG
                     SGITPVMGMLRNHIAELPDVVMIHSAPTARDVIFATELRELAATGLMRLVELHTDTDG
                     MLDLAVDLDRLVPDWREREVWACGPAGMLDACETHWSTAELRERLHVERFRTHLHAAG
                     EGGTVTFTKTGTIVEADGGRPILEVAEEAGMLMPHGCRMGICYQCVLPLRDGAVRDLR
                     TGEVTYAAPGDGVAIQTCVSAAAGACEIEH"
     gene            13030..14373
                     /locus_tag="PLK64_01380"
     CDS             13030..14373
                     /locus_tag="PLK64_01380"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HPV78420.1"
                     /translation="MTLVAEPHPVATDPTMPSERSGQRAGSAFAPPAPPVDARVNPHL
                     RLVDGKERVLHPKPSGRPDPTAHLSAADIEALGRELDAIRQTVMDSRGADDAAYIRKV
                     IAFNRYLELTSRAILLFSLFPPAWLVGTAGLSVAKIIDNMELGHNILHGQWDWMRDPK
                     IHSTTWEWDHVTPAAQWKESHNETHHTYTNVLGRDNDLGYGIMRVDADQPWHLANLGQ
                     PLYSFINALFFEWGIASYDLKLHDVITGKMPREESKPRVDAMLTKMRRQVLKDYLIHP
                     LLSGPSFFPTMGANFVATIIRNLWSNGVILCGHFPFGVDTFAYDSIEGETRGEWYVRQ
                     MLGSANISGGWLVHFMSGNLSHQIEHHLFPDLPSNRYAQVAPQVREICQRYGLHYVTG
                     SYPRQLASAWAKVFVYALPDKEPDRSRGQIVASAVKDAFVAGKAKRAAKRAAKLA"
     gene            14523..16655
                     /locus_tag="PLK64_01385"
     CDS             14523..16655
                     /locus_tag="PLK64_01385"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HPV78421.1"
                     /translation="MARIPDYLDFDLELTDAGEGGYTARVLASPKGEASADFRMPFLG
                     VDLENVILKLGRTRSGVRALGSPQHDLARTFGGQLYDTVFSGEVGTCFRRSLDEADEQ
                     GKGLRIKLRLTDVPSLADVPWEYLYAAGLGRFLVLSESTPIIRYLDLPREVLPLTVTP
                     PLRILLAVCSPTNLTQLDTQAEVARVAESLRDLVERGLVIVDPLPNATLTALRQALRA
                     TDYHVLHFIGHGGFDPVAGDGVLAFEDADHLGHLVSGQDLGTLLHDMRTLRLAVLNAC
                     EGARQSPTDPFSGVAQCLVRQGLPAVVAMQFEITDTAATSLSSEFYAALADGLPIDAA
                     LAHARMSVFAADNDVEWGTPVLYLRARDGRIFDVATQGAAPPTPTPTPVATPAPTPAP
                     APPSPIPIPYPISTTPPTPAPMPVPTSATPPPMPVPTPPTPQTGAGGIPADAPGAPKQ
                     RHPAVAPAVVAAVIVTLGLVAAFLLRPDGGAATNGPGPAPKEFNGFVAMRGTPVIDGQ
                     ANEWAGAVPYVSDVLVAGKATKIKATWSLMWDQQAFYVVADVADPNLMPADQTAPDQQ
                     WRGDSVSFELGADPRGLAVEAGLRATDAHYIVGLTPTTPARTKIVINRANVTKNTIDA
                     GRFHAESDAVVALIPGGYRVEARLPWAVTGVAPLGVQTGQVFGINLNASDAGSSPKEF
                     AAMYSSNRGRTSANQVRPAAWQVVQLQG"
     gene            16775..16993
                     /locus_tag="PLK64_01390"
     CDS             16775..16993
                     /locus_tag="PLK64_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78422.1"
                     /translation="MARYDITTATLGTLLADPDVVAIMEKHAPGITTNPMIGMAAGMP
                     AAQAVAMAGGMIGGDAVSAITDEVTSLN"
     gene            17241..18221
                     /gene="ligD"
                     /locus_tag="PLK64_01395"
     CDS             17241..18221
                     /gene="ligD"
                     /locus_tag="PLK64_01395"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HPV78423.1"
                     /translation="MAAKATTPAIELAVGERTIRLSNPDRVYFPEHGWTKLDLANYYL
                     AVGDGIVRALRERPCMLHRFPEGLAGEKVHQKRIPHGAPPWLQTVRLHFPRWNRTADE
                     LCVTELGSVIWAVQMSTVEFHPWNVRRADVDRPDEWRIDLDPMPEAGFDRVRVVAGVV
                     REVLDELGIVGFPKTSGGNGLHVYVRIVPEWEFGDVRRAALAFAREVERRLPGDATTT
                     WWRKDRDPRHVFIDYNQNARDHTIAAAYSVRGVPDGTVSTPIRWDEIADVEPRSLTLA
                     TVPARYAALGDLHAGIDDTAYRLDALLEWAERDERAGADTPPEPDAGPDT"
     assembly_gap    18529..18628
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18633..19391
                     /locus_tag="PLK64_01400"
     CDS             18633..19391
                     /locus_tag="PLK64_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPV78424.1"
                     /translation="MLTVVLVALAGLSGTLLVRDLRAPAPLTQAEIDKAAAKVVDKAL
                     AEAARAPSAASTAYAQVAASLVIIETVGGGGDPAAQGLGTGVIVSQEGAILTANHVVS
                     GGGAITVTFADGTRAPARVQSAQPENDIAVLVADQLPQVVVPAVLGGGVRVGDPVYAV
                     GHPLGLTASLSSGVVSATGRTIEAEGLKLADLIQFDAAVNPGNSGGPLLNSAGQVVGI
                     VTALANPSKQPFFVGIGFAVPIATAGGAAGGPSI"
     gene            19400..20482
                     /locus_tag="PLK64_01405"
     CDS             19400..20482
                     /locus_tag="PLK64_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPV78425.1"
                     /translation="MSTPFAAGTSAAAPSPGPGAHPLEQMLYQVKRTIVGQDQLLERM
                     LVALLARGHLLVEGVPGLAKTMAIKTLAEAIGVDFKRIQFTPDLVPADLIGTRIYNQQ
                     TGDFQVSLGPVFANLVLADEINRAPAKVQSALLEAMQERQVTIGRETFSAPDPFLVMA
                     TQNPIESEGTYPLPEAQVDRFMLKVLVGYPSATEEYVVVERMIRAAERIDAVLDADGL
                     RGLQAAVDTVYVDPGVIQYAVALAGATRDPGVVGLPELARYLSFGASPRASINIVLAA
                     RAWAYLRGRDYVIPMDARELAPDVLRHRMVLSYEALADDVSADDLLGPILAAVPLPET
                     AGGRHTSGVAEPIPANVDPAWYRRPE"
     gene            20461..21357
                     /locus_tag="PLK64_01410"
     CDS             20461..21357
                     /locus_tag="PLK64_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPV78426.1"
                     /translation="MVPTTGVAAPERLLRRLEWTVVRRLDGRLQGDYRTIFRGTGVDF
                     RDLREYEPGDDLRHIDWNVTARTDVPFVREYTEDRELTAWLLLDRSASMAFGPTDRPK
                     HVVLLELATTLALVLARGGNRVGAVLYGDGVDRTVPPRQGRDQVLRIARELLADAPTA
                     GHPTDLGGLLRAALGMARQRSLIVIVSDFVTTPGWERPLGLLARRHDVVAIQLVDPRE
                     HDLPEAGLIALTDPETGEHVLVDTGDVEFRSRLWELAQARQRQLEAVAAHAGIALHPV
                     STDEDLVGALLRMARLRSKWRR"
     gene            21363..22349
                     /locus_tag="PLK64_01415"
     CDS             21363..22349
                     /locus_tag="PLK64_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPV78427.1"
                     /translation="MTFISPWALLGLLLVPGLAAAYRIVRARRAARRAELAALGFAPV
                     LAGGRRRRGWVVPTALGLTLGLLVLAAARPQATVPDLRREGTVILAIDASTSMTATDV
                     APTRMAAAKAAAKAFIARQPSTIRIGVVSFGDSAVVLQAPTLVQAEVVAAIDRLTPQG
                     GTSLGQGIFASLKAIAGGKLAITEEQLAGDIESLDIGYFGSSAIVLLSDGEDTSRLDP
                     LALAELAGVAGVKINTVGLGDPAGTTVTVNGFTAATQLDEATLTAIADATGGRYRAAP
                     DEASLADVYGSIDLAMTSTPVHIEISALFAAAALLVLVAAASTSILRTGRVI"
     gene            22350..23501
                     /locus_tag="PLK64_01420"
     CDS             22350..23501
                     /locus_tag="PLK64_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPV78428.1"
                     /translation="MSFAWPYALLAVLLAPALLAAYLWSLRRRRRYAVRHPDLALVRA
                     ALPARSRWRPHLPVALLVLGVGLLGLAVARPQASVEVPIQRSTIILALDVSRSMCATD
                     VAPNRLSAARTAVQEFVETQPRDTRFGLVAFSGSAALVVPPTTDSAAVLTSLDGLSTG
                     RGTAIGSAILTSVDAIAAINSQVQPIGEVPLGPDTMQQPGVPAPVPSSIDPPPGATGG
                     FVSDIVVLLTDGANTRGVLPVAAAKLAAERRVRVYPIGFGTTNPTQMVCTAEQLGGDA
                     FGEFGPRSGGQVPGGQAPGGQAPGGGFGGGRNFLVVDEPTLKAVAATTGGAYHQASDA
                     AQLGEVLRGIPREVVLQREDLEVTAYLAILAALVVAGALVLSVRWNPLA"
     gene            23809..24444
                     /locus_tag="PLK64_01425"
     CDS             23809..24444
                     /locus_tag="PLK64_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009357634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HPV78429.1"
                     /translation="MDRFELKKDYPSLYAPRSRTWEVLDVPPMLAIAVDGAGDPNMSP
                     DYVEAVSALFSVAYTIKFAAKAQGREMVVGPLEGLWWAEDPRSFIERDKGAWSWRMLI
                     VQPPWVDEVTLEAAVAKVAAKQAVKGAGLGSTALSKLTLVTLNGGRCAQVLHIGSYDD
                     EGPTLADLHDRFMPEHGLAFNGPHHEIYVGDPRRTAPEKLKTVLRQPVRPA"
BASE COUNT         3497 a         8636 c         8378 g         3842 t
ORIGIN      
        1 gacaatgcag acaatgcaga cgacgctgcc gacatggccc tggcgccgac gatcaagcgg
       61 acgctgctca gcgattcgag ccaaccgatc gacgtcgacg accggcccgc acaatctcca
      121 cccgggccgg cggccaatca agaatccccg accgtatctg ggtcctcccg caagccagac
      181 cacaggaagc gggttgcggc ggtcgtgatc gccggcgcgg ctgtcttgac cgcgatgggt
      241 accttagcag cgacgaccag gccggtctct gctccaggtg tcgtggcggc gccgacttct
      301 acgggcaagg gcatcgacat ggtcggccaa ggcccaatag agcgattgat cgtcaaaccc
      361 acccccgaat ctctgcacgt gtcagtggaa ctctcgcgcg tgctcgacgc gaatagtgag
      421 ctctgggtgg atattcagtt cgcccgaacg gctggcaact gtcccgaccg gattattgtc
      481 ttttccccca agcagcggca ggcgactctc ggaacggcga cgtgtcaggg tgaggtggtg
      541 acctacagtg tcgcgacaac tctgtcgtca acgcttgagc cggggtcggt caactcagcg
      601 gccttgtcgt gggcggcgct cgacggtcca agagggggaa tcctgattgc tagggtgcgt
      661 ctggtgaccg aaggccagac agtctcgtgg ctgccgacca atcagtcgta tgcgaccgcg
      721 agtacgggcg gaggcgtcgc tattccgaag tgagtcagcc cgtgccgatg actttgctgg
      781 gaagtgtgac ctcgccaccg cgtgttcgcg aggcacggat cgtttgctcg cgctcagcgc
      841 agcattcacc ttcgtgcggg actcgcctga ccgccggcaa ccacgagagc cgggcgcagg
      901 tcggcgtcga gcacgagcac gtcggcgacc ccaccggtga ccaggtgtcc gacgtccaga
      961 ccgaaggcct tggcaggcgt gccggtggcg gccaggacgg cgtcggccag gtcgatcccg
     1021 gcggtctgca cggcccagcg caggcaatcg gcgacgtgcg cgacgctgcc ggcgatggct
     1081 ccgctgctcg tgagccgggc ggtgccgtcc gccacgacga ccggcaggcc gccgagcgtg
     1141 tagttgccgt ccggcagccc ggtggccgcc atcgcgtcgc tgatgaggtt gatcgaccca
     1201 gggccgacca cctcgaagag catccgcacc gtgtcggggg acaggtggac gccgtcggcg
     1261 accacctcaa gaacggcgtc accccgcagg gccgcgcgga tcgccccggc agccgtgccg
     1321 ggcgagcggc cacggatcgg cggcatcgcg ttgaagatgt gggtgacgag ggacggggct
     1381 cccgcggtgg ctgcggcccg acccaggacg tcgaaggtca gcgtcgcgtc cgcctcggtg
     1441 tgccccacat tgacccgtat gccgtgtcgg tgcagcgccg cgggcagcag gtcggcgccg
     1501 gctcggtccg gcgcgaaggt catgtggcgg atcgccccag gagcgccgac cgctgcggcg
     1561 gcctcggcca gggcgtcgac cagcccggga tcgccgtccc gcagggcatc cggatcgtga
     1621 gcccccttgc gggctgcggc gatgaaggga ccttcgaggt ggatgccggc taggtcgccg
     1681 gaggcgacga gagcgctgag ggtctgtgtg gcagtgatca ggtcggaggc cccggccgag
     1741 accaggctcc cgatgagcgt ggtggacccg tgcgcccggt gttcggcgac ggccgtgcgg
     1801 gcagcctcgg cgtcgacgcc gaactccccg ccagcgccgc cgtgcaggtg cgagtcgacg
     1861 agcccgggga cgagcgtggc gccggtcggc accctcgggt cgggcgcgac tgcggaccag
     1921 cccggtggca gctcggccag cgggccggca tacgcgatgg tcccgtcctc gatcgcgacg
     1981 gcgccgtcgt gcgtggttgt cccgccggtg accagccgac cggtgaggag ggtgcgcgtc
     2041 gggccggagt cgccgtcggt cgtcatgacg ggaccatcgc ccggctggcc gccgcctcgc
     2101 tgccgaggga catgctgacc tcgtaacgat cgccgcgata ccaggacagg acgtattcga
     2161 ccgggcggtc accggcggag gcgacccggc ggaagacgag cagcggagtg tgctccgggc
     2221 aggcgatcag gcgggcgaca tcgccggttg cggcctcgcc ccagagggtc tgctcggccc
     2281 cgtcgatggt cagcccgtac tggttctgga acagcgcgta gagcgagctc tcgaggtcgt
     2341 gctggtccag gcccggcagg aggctgacgg gataccaggc gtcctcaagg gcgatggcct
     2401 gtccgtcggc gagccgcaac cggcgcagtg accaggcccg gtcgaggggt ccgagcccca
     2461 gggcgcgggc gattgggtcc gggggcacct cacgacggat cgacaccaac cgggtcgagg
     2521 gcaccatgcc gcggcgatgc atgtcctgag tgaaagacgc caggtgcagt tgggtgccga
     2581 ggcgcggccg gatggcgaag gttcccaacc cgtgggtgcg ttggagcaac ccctcataga
     2641 caagggtgtc gatcgccttg cggaccgtgg ctcgcgagac gccgtaggtg gcggtcaggt
     2701 cgcgctccga agggatggcc tcgccgggga cggcgaggcg ggccaggacg tcgcgaagct
     2761 gccgatgctt tggcaccggt ccctccaaga tctgatccgg cagcgtgacg gctgtcatgg
     2821 ttaggtccct ccccacgtgg acaaggtggt cttcattcgc agcccgggcg gcaccgcccg
     2881 cccgaacgcg agagtcgaat cgagagtcga atcgagagtc gagacgacgt ggatacggcc
     2941 tcattctcta cgctgcgtgg ccccgtccgg gtcgctgtcc gcgggccacg tgagcaagtg
     3001 ttcgccgatg acggcgtcgg gtccgcacac ggcgtcgacc ggggtctcac cggggacgtc
     3061 gagcaggatg atcggcacga ccgggctgcg tccgccggcc tcgatatccg ccggatccca
     3121 cgtgacgatc gggtcgccgg ctgcgatgtc ggtgcgctcg gtggcgtgca gggtgaaccc
     3181 cgcgccaccg agttggacgg tgtcgatgcc caggtggacc aggacggcga catcggctcc
     3241 ggcgatgacg aacgcgtgcg ggtgcagctt gaccaggcgc ccggagatcg gggccacgac
     3301 cgtggccgcg ctgcggtccg ggtcgatggc cacgcccggc ccgacgatcc ggccggcgaa
     3361 gacggggtcg gggacctcgt cgagggcgat gacccgtccg ctgcaggggc tgaggatcgc
     3421 ggtcacatga ggtcctggat gtcctcggcg aggttgtccg cctcggggcc gacgacgacc
     3481 tggacgatgt tgccggtgac gatgacaccg tgggcgccgg ccgccttgag ggccgcctgg
     3541 tcgacgagcc cgccgtcttc gacctcggtc cgaagccgcg tgatgcaggc ctcgatctcg
     3601 acgatgttgt cggcgccacc gaggccggag aggatgcgtg ctgccttgct catttgagtt
     3661 cctttcgtgg gatcactgtg cggttctgcg tggatcactg tgcggttcta cgggatatca
     3721 gtgcggatct atcgggtcgg tcagtggacg aattcggtct gggtgacctt gcgcaacccg
     3781 cgaggggcgg gagacgaaca ggacccggcg gccggcggcg agggccgcga tctcgtcggt
     3841 gcgcgacagg tgcgcggcca gggtccggcg aaccgcctcg ggctgctcgg cgatctctcg
     3901 ggccatcgcg gtggctgtca ttcggagcgt tctcctcggg gcttgacaaa cggtgggagc
     3961 ctgggtcatg cttactggta cgtaccagag cgtatcagta cggaccacga acagcaagcc
     4021 ccgcagaagc cccgcagaag ccccgcaagt gcactgaaga accaccgaag tagcaccgcc
     4081 gtagttcctc aggagcacgg cgagaagccc ccggctccac cctcccgatc gctcgtccgt
     4141 cccgaaagga ctttgatgaa cgcccaagcc gccgcccgcc agacgagtgg gaaacggttc
     4201 gatcttgcgc cgctgcagaa gttcggtcgc agcctcatgc tccccatcgc cgccctcccc
     4261 gcggccgccc tcctcctgcg catcggccag cccgacctgc tcggcaagga cggcctcggt
     4321 tggacccagg tggccgccgt cctcggcgca gccggtgacg cgatcttcgc caacctgccg
     4381 ctgctgttcg ccgtcggtgt cgccatcggc atggccaaga aggccgacgg ctcgaccgcc
     4441 ctcgccggtg tcgtgggtta cctcgtcttc aagggggtgg ccgacgcgat ctcaccgttc
     4501 gtcctcggag ctgccgccgc cggcgagaag cagaaactca tcaactatgg cgtcctcggc
     4561 ggcattgtca tgggcatcac cgccgccaaa ctctgggagc gctaccaccg gatcagcctc
     4621 ccgccctacc tcgccttctt cggcgggcgg cgcttcgtcc cgatcgtcac ggccctggcc
     4681 gcgatcatcc tggccgtcct gctcggcgtg atctacccgg ccttcaacag cggtctgacc
     4741 gcgctgggca actgggtcgt ggccaacacc atcatcggtg gtctcgtcta cggcacgctc
     4801 aaccgcctgc tcatcccgct cggcctgcac cacatcctca actcggtgcc gtggttcgtc
     4861 ttcggtgagt acaccgccgc cgatggccag gtttaccacg gtgacatcgc ccgcttcctc
     4921 cacggcgacc cggctgcagg ccccttcatg accggcttct tccccatcat gatgttcgcc
     4981 ctcccggccg cggccttcgc gatctggcgc gcggccaagc cctcccagcg caaggccgtc
     5041 ggcggcatca tgctctcggg cgccctgacg tcgttcctca ccggggtgac cgagccgctg
     5101 gagttctcgt tcatgttcgt cgcctggccg ctctacgtcc tgcactcggt cctcaccggc
     5161 acgtcgctgg cagtcgccaa cgccttgggc atccacgacg gattcggctt ctccgcaggc
     5221 ctgttcgact tcgcgctcaa ctggaatatc gccaccaaac cggccctgct catcgtgctc
     5281 ggtctgatct acgcggtggt ctactacctc ctcttcacct gggtcatccg caagtggaac
     5341 ctgcggaccc ctggccgcga ggacgacgac tcgacgacgg ccgacaccag cgcctgatcg
     5401 tcacagacac ccgcttgcgg cgggtcacgc agcagccggt ccgagagccc gctcggaccg
     5461 gctgtttcgc gttgacctca ccttccggcg gggccgggtt acgctccggc tcatgtatgc
     5521 cgtccgccgc accnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngacggga
     5641 cggcggtggc gctggacgtg accgtcttcc gtccaggcgg gctcggccag cacagcgaac
     5701 cctggtcggc ggtcctgctg gcccacggct tcggcggcag caaggccgac ctggtcgagc
     5761 gaggccgtga cctcgcctcc cggggctatc tcgtgctcgg ctactccgcc aggggtttcg
     5821 gcgcgtcggg aggccggatc cacctcaacg acccggcata cgagatcgcc gatgcccgcg
     5881 ccctcgtgag tctgctcgcc gcccgcagtg acgtgctcct ggatgcgccc ggcgacccgc
     5941 acgtcggcgt ggtgggggcg tcctacggtg gggcgctcgc gatcatgctc ggcgcgaccg
     6001 accagcgcgt ggacagcgtc gtcggagtcg ttgcgtggaa cgacctggcc gacgccttct
     6061 tcccgcagtt cgcagtcgtg gggccgcgca gtccgttggg gccggctcag gtggatcggt
     6121 ctcccgagcc gggaccgctc aaggcgctct gggcgtcccg attcttcggc tcggccctcg
     6181 ccagtgcggg ttcgccgggt gctggggttg gtgcgggtgc tcacccgagt tcgggtggcg
     6241 gcgcgggcgc cgtgtgtggg cgattcgacc ccaccgtgtg tcggctgttc ctggaggcga
     6301 cggtgaccgg gcgaccgagc cccgagttgt tgacggtgtt gcgcgcccac agccctcgtc
     6361 cgctgcttgc cggcctgcgc gcgccgacca tgctcgttca ggggatggcc gactcgctgt
     6421 tcggcatcga gcaggccgcc gcgactgcgc gagcgatcgc ggtccaggtg ccgcggctgg
     6481 cgatccggtg gatcgacggt ggtcacgacg ggctgtcgag caccgccgca gccgacgagc
     6541 aggcgttgcg cgattggctg gccgacaccc tccgtggcac cacgcttctg gccggcgcgg
     6601 gccagttcag ctacgccgca ccgattccgc ggcgacggac ggtggctccg ctcttcacca
     6661 cgcccgactc tccgccgaca gcgacctgga ccgccgtgcc ccttgcgccc cttgtcggcg
     6721 cctcgggggg cgggacgtcc gacccgcagc ggatcgtcac gccaccgggt ggtcagccgg
     6781 cgtcggtgac cgcgattccg tcgctcgggg ggctgggcgt gggcgcggcg gcataccaac
     6841 tggcagctct cccagggcag tccgccgcct tcgacagtcc tcccttcgcg cagcagaccg
     6901 ctatcgtcgg tacgccgcga ctaaccctca ccgtgacctc gaccgggccc gagaccgtgc
     6961 tcttcctgtc gctgtggcaa gtgacggccg gccaggccac cctgccgcgg cggctggtcg
     7021 cgcccgtgcg ggtgctcacc acgccgggac agccgacctc ggtcgacgtc gcgctcgctc
     7081 cggccacctg gacggtcgag gcgggtagct cgcttcgggt gttggtcacc tcaaccgact
     7141 cggcgtatgc cgccccccgc gaggctcgcg tcgacctggt ggcggtcgcc ggcggggagc
     7201 ttcgactgcc gcatgtcgac ggcgacctcc ttgctgcgga gagcgacctg gacaccgagt
     7261 cggtcggggt cgcgacggca atcgcgctgc tgctggccgc tttcggggtg ttggcctggc
     7321 gcgaacggcg ccaacgacgg ttgctgcccg accgcgcgga cctcgccgac gtgccgctgg
     7381 ccgtcgagca cctcgtgaag acctacgccg acggtcaccg cgccgtcggc gatgtgtcgt
     7441 ggcgggccga acgcggccag gtcgtcgggc tgctgggacc caacggggcg gggaagacga
     7501 cgacgctgcg catggcgatg gggctcatca cccccgattc tggtgcggtc tatctcggcg
     7561 ggcgggcagt gcgtccaggg gcaccggcgc tgcgcggcgt cggggcgctg gtcgaagggc
     7621 cgggcttcct gccgcacctc accggccggg ccaacctgca cgcgtattgg gcggccaccg
     7681 gtcgcccgat cgaggaggcc cggctcgacg aggccctcga cgtggcggcc ctcggcggcg
     7741 cggtcgaccg gccggtgcgc tcctacagcc acggcatgcg gcagcggctc ggcatcgccc
     7801 aggccatgct cgggctgccc gacctgctcg tcctcgacga gcccaccaac gggctcgacc
     7861 ccccgcagat cgccgcgatg cgcccgatcc tgcagcggta tgccgccgcc ggccgcaccg
     7921 tcgtcgtctc cagccacctg ctggccgagg tggagcagac ctgctcgcat gtcgtcgtca
     7981 tgcacgccgg gcgggtggtc accgctgggc cggtcgccga tctcatcgac tcctccgaca
     8041 cgaccgtcgt ccacctcgac cccgccgcga cggcggaaac cattgcggct gttgctgatc
     8101 ggctcctgtc ggtcgccggg atccttgagg tcgagatcgt cgaggacgag ggtgactcgc
     8161 ggctggtcgt caccgcgggt atgccgcgcc ccgacgtggt ccgggcgctc accgaggtcg
     8221 gtgccgacgt cgtcgggctg agcagccgca aacatctcga ggaggtcttc ttgcgggtca
     8281 tcgccgccgc ccagaccgca ggagagccga ccggatcggt caccgaacgg ctgcgacagg
     8341 tgcgggcccg atgaccacgg acccggtcgc caagcagccc accaccacgg tgccgaccgc
     8401 cacggagccg agagcgacgg agccgagcgc gacggagccg agcgggcggc atacccgagg
     8461 ggcgttgtcc ttcggcgcgg aggtgcgccg acaactgggc cgccgtcgca cgctgtggag
     8521 tttcgtcatc atgcttgcgc tgccggtgat cctcattggc gcgttcgcgc tcggccgcac
     8581 gagtggcccc ccgggcggct cgcggctgtc ggacctcgcc cagcagggca gtgccaactt
     8641 cgcgatcttc gcgctggcgg cagcctccga cttcctcctc gtcgtcctcg cggccctctt
     8701 cgcgggcgac gcggtgccgg ctgaggcgtc ctggtcaacc ctgcgctacc tcctcatcgc
     8761 gcccgtcccg cgggctcggc tgctggcgag caaatgggcc gtcgcgatcg tcagcctcgg
     8821 ggtcgccatc gtcgtctatc tcggttgggg gctgcttgtt ggcggtcttg cctatgggtg
     8881 ggctcctttc accaatccgg ctgggggagt gttgggttgg ggcgagttgt tgccccggct
     8941 ggcgattgcg acggcctatc tcttcgtgac cctgctgcag gtggcgggga tcgcgttcgt
     9001 gctcggtgtg cggaccgacg cgccgctggg tgccgtcggg gtggccgtgc tggtcgcgat
     9061 cgtctcgtcg atcctcggcc agatcgacag cctgggcgac ctgcgcaacg gcctcccgct
     9121 gcactacatc cgcgcatggc aggacctgtt gacgccccag gtggactgga ccgcgatggg
     9181 gcacggggtg ctctggtcgg tgctctacac gcttctcacc gtcgggttgg ccttcgccgt
     9241 gttccgccgc aaggacgtcc tcagctgagg cgaccttctc gaccgggctc gcggcggcgg
     9301 cccgccgagc cccagcgagc cctgaccacg attgtcagcg tcgtgacgga cgtctcgtgg
     9361 aaggcggatc acacatcgag cgttccctgg gggtgacctg tcacgctgtt actgacgggt
     9421 aacactgaaa tcaacgaccc tggggggcga cgatgctgga tccgaaggcg gcgggggaga
     9481 tcgcccgcgc attcgggctg ggcgcgaacg cccgcctcga aggcccagtt gcccatggcc
     9541 gcaagggcga agtctggcgc ttggtcaccg atgagggtcg gtatgccgtg aaggcccttt
     9601 tcggcacggt gttccgcgag cgggcggagc gggacgcggc ataccaggac gtcgttcgcg
     9661 atacgggcgt accgatgccc gcggtcctgc gcaccctctc gggcaacgtc ttggccgacg
     9721 tcggtgggcc gccccccaac gcgaccgtcc gggtctacga atgggtcgac atcctcgagc
     9781 cgcgccgtga ccttgatccg cgcgagatcg ggcggctgct cgcccgcgtg cacaacgtcg
     9841 aggcgcccgc tgccggaact gtggatccct ggtaccgcga gccggtcggc cctcaggtct
     9901 ggggcgagct cgtcgaggcc ttgcgtgcgg aggacgcgcc cttcgccacc gacctcgccg
     9961 cgctggtgcc ccaacttgtc gctcttgagc agactttcga ggtgcccgcg gacgtccgga
    10021 cctgccactg cgacctctgg gccgacaacg tccgcgcgac gcgtgacggc gggatcgtcc
    10081 tgctcgactg ggagagtgcc ggccccgaga gcccgtcgca cgagttgggg atggtcgtgt
    10141 tcgaatacgg ccttggcgac ccggcccgga tgctcgacct gtatgccgcc tacctcatcg
    10201 ccggagggcc cggccggatc tcgcgcgccg gtgacctgac catgttgggg gcgacgagcg
    10261 agcacctggc cgaggaaggc tgccgccgct ggctctcggc gaccgatgag ggcgaccgcg
    10321 agctcgccgc cgcgtgggtc gaatggctgc tgcacgagcc gatcacgccg gccaccgtgg
    10381 ccgacgtcct cgacgctgtg gccggcctcg gtgggagccg cggggcgtag cctcgactgg
    10441 ccgcgccgca cggcatacct gatcgcggcg ctgggccggg gtcggggccg cgcatgaagc
    10501 cttctgcggg ggtgtgatta ccccaccggg ggctgcttca tcggctgcct cacccccggt
    10561 ctcacgccgc gcgctcaggg cgtcggtggc cgcgtgggtt gcagcgccag cgccatcgtc
    10621 agccgcagga cctcctcggg gtcatcaagc cggtccccga acaactcgcg cagctgggcc
    10681 atgcggtagc gcaccgtctg tgggtggacg aacaggtggg cggcgatgag ctcgcgacgg
    10741 ccgtggtgca gcagccacga ccgcagcgtc tcctccagcc gcagccgcac ggtcgggcgc
    10801 accgcggcga gcggcgccaa ggcccgagcc cgcaagtcgg ccagcgcctc agggtcggcg
    10861 ctcagcagca actcggccag gaggtcgtcg gtgtcgaggg ttccgcgcga gttgggctgg
    10921 gggaggaact cgcgagcgcg caacacccgc tggaacgacc gatgtacctg cagccacggt
    10981 cgctcgggcc cgatgatcgc cccgcgccct tccaactggc gcaccagccg ctcccgatcc
    11041 cgcccggtgg cgtcgggcac gagcaggacg ccggcgtcct cgtcgtcgac gccggccggc
    11101 tcagtcgaca tcgccagggt cctggcgtcg aggagcccga gcaccgcgcg cagttgcccc
    11161 tcgggcagga gcaccgcggt gagcgaccgt ggcgggtgcc agtcggcccg ccgggcggcc
    11221 gcgacgaggt cggcctcctc gccgccaccg acgagcagcg cggccaatcg gtcgcgatag
    11281 cgctcgcgcg cgcgcccggc agtggccagt tcgtcggcat gacccgagat gctcgcggac
    11341 gacagggcgt cgatgtaggc gaacaccaac tcggcaaacg cccccagcgt ggccgccggc
    11401 accccggtcg ccactgcctc ccgcgacaga ccgcgccagc agactcgcgc cccgacccgg
    11461 tatgccgcct gcagcgcgtc catggaccgg ccactgcggg cctcgccgcg gcccaactca
    11521 taggctgcag ccgtgcttgg ggcgatgggt tcggcagggt cggtgccgct gccgacccgg
    11581 gccagctcga ggaaattcac cagggccagc tcgacggcgg tacggatgcg cgcacccatg
    11641 gcgtccgaaa acggccgggc atagctgggc acttcgcgca tgatggtctc gacggcctcg
    11701 gcagccaccc gcgggacgac gcctcggagt ctctcgaaga cgtcctgttc gagggccaag
    11761 gacgtgaccg ccggcaccgc gctggggggc gttcggggag tgcgaggggc ccgtggggtg
    11821 acggacatgg cttcaccgcc ttgtctctcg cgaacaaaag ttcagctccg cttcacgtcg
    11881 agccatcaga aatctagcgc agaaacccgg aacaatgggt tctatgtccg ccacggtcac
    11941 ctcgccgcga ggccgcagtc ttcgacaacg cgcctggcgc gccgccgaga tgctcactgg
    12001 agccctcatg ccggccgaca ctctcgatgt catcaacccc ttgcgccccg gcgccgaccc
    12061 gctcgcgggc cggattgagc ggattcatct cgaaaccgcg gacgctgcaa ccgttttcat
    12121 caaacctgga gccgactggt cggggcacat ccccgggcag tacctccgct tcggcatcga
    12181 catcggtggt gtcaggcagt ggcgcaccta ctcgctgacc agccggctcg accgtcccga
    12241 cggcctgctg acgatcacga ccaaggccat ccccgacggc ttggtgagca accacgtcgt
    12301 ccgtgacctg cgcccaggca cgctggtcca catcggccag gccgaaggcg acttcgtcct
    12361 cccgacggcc cgcccggaca aggtcttgtt cctcaccggc ggcagcggca tcacgcccgt
    12421 catggggatg ctgcgcaacc acatcgccga gttgcccgac gtggtgatga tccactccgc
    12481 gccaaccgcc cgcgacgtca tcttcgcgac cgagctgcgc gagctggccg ccaccggcct
    12541 gatgcgcctg gtcgagttgc acaccgacac cgacggcatg ctcgacctcg cggtcgacct
    12601 cgaccgcctc gtccccgact ggcgtgagcg tgaggtctgg gcgtgtggcc cggccggaat
    12661 gctcgacgcg tgcgagaccc actggtcgac cgccgagctg cgcgagcgcc tgcacgtcga
    12721 gcgcttccgc acccacctgc acgccgctgg cgagggtggc accgtcacct tcaccaagac
    12781 cggaacgatc gtcgaggccg acggcggccg cccgatcctt gaggtcgccg aagaggcggg
    12841 catgctcatg ccgcatggtt gccgcatggg catctgctac caatgcgtct tgccgctgcg
    12901 cgacggcgcg gtccgcgacc tgcgcaccgg cgaggtcacg tatgccgccc ccggcgacgg
    12961 tgtcgccatc caaacctgcg tgtcggctgc cgccggcgcg tgcgagatcg agcactgagg
    13021 agccactgaa tgaccctcgt cgccgagccg caccccgttg cgaccgaccc cacgatgccg
    13081 agcgagcggt ccggccagcg ggccgggtct gccttcgcgc cccccgcccc gcctgtcgac
    13141 gcccgcgtca acccacacct gcgtctggtc gacggcaagg agcgggtcct gcaccccaag
    13201 ccgtccggtc gtcccgaccc gaccgcgcac ctgtcggccg ccgacatcga ggccctcggt
    13261 cgtgagctcg acgccatccg ccagaccgtc atggattcgc gcggagccga cgacgcggca
    13321 tacatccgca aggtcattgc gttcaaccgc tacctcgagt tgaccagccg cgcgatcctg
    13381 ctgttctcgc tcttcccacc ggcctggttg gtcggcaccg cgggcctgtc ggtcgccaag
    13441 atcatcgaca acatggagct gggccacaac atcctccacg gccagtggga ctggatgcgc
    13501 gaccccaaga tccactcgac gacctgggaa tgggaccatg tcacgccggc ggcccagtgg
    13561 aaggagtcgc acaacgagac tcaccacacc tacaccaacg tcctcggccg cgacaacgac
    13621 ctcggctacg gcatcatgcg cgtcgacgcc gaccagccct ggcacctggc caacctcggt
    13681 cagccgctct acagcttcat caacgccctg ttcttcgagt ggggcatcgc cagctacgac
    13741 ctgaagctgc atgacgtcat caccggcaag atgccccgcg aggagtccaa gcctcgggtc
    13801 gacgccatgc tgaccaagat gcgtcggcag gtcctcaagg actacctcat ccacccgctg
    13861 ctgtctgggc cgtcgttctt cccgacgatg ggcgccaact tcgtcgcgac gatcatccgc
    13921 aacctgtggt ccaacggggt catcctctgt gggcacttcc cgttcggcgt cgacaccttc
    13981 gcctacgact cgatcgaggg cgagacccgc ggcgagtggt acgtccgcca gatgctcggt
    14041 tcggccaaca tctccggtgg ctggctcgtc cacttcatgt cgggcaacct cagccaccag
    14101 atcgagcacc acctgttccc cgacctgccg agcaaccgct acgcccaggt cgccccgcag
    14161 gtgcgtgaga tctgccagcg ctacggcctg cactacgtca cgggttccta cccgcgtcag
    14221 ctcgcctcgg cctgggccaa ggtcttcgtc tacgccctgc ccgacaagga gcctgaccgc
    14281 tcccgtgggc agatcgtcgc ctcggccgtc aaggacgcct tcgtggcggg gaaggccaag
    14341 cgcgcggcca agcgcgccgc caaactggct taaccccttg cgggcaggga tagcctcaca
    14401 gacgtatctc tcgatagtcc ggacacctct ttctggtgtt acgtcgtgag gagcgtctct
    14461 cccatgctca agccaagtgc ccatccgcac ccgatgatca ggttgtccga gcgaggcctc
    14521 tgatggcccg catccccgac tacctcgact tcgacctcga actcaccgac gccggtgaag
    14581 gcggctacac cgcccgggtc ctcgcctccc ccaaggggga ggccagtgcc gatttccgta
    14641 tgccgttcct cggcgtcgat ctcgagaacg tcatcctcaa gctcggccgc acccgttcgg
    14701 gcgtccgcgc gctgggctcc ccgcagcatg acctcgcgcg caccttcggc ggtcagctct
    14761 acgacacggt gttctccgga gaggtcggca cctgcttccg gcgcagcctc gatgaggcgg
    14821 acgagcaggg caagggcctg cgcatcaaac tgcggctcac cgacgtcccc tcgctcgccg
    14881 acgtcccctg ggaatacctg tatgccgcgg ggctgggtcg cttcctcgtt ctttccgagt
    14941 ccacgccgat catccgttac ctcgacctgc cgcgtgaggt tctgccgctc accgtcacgc
    15001 ctccactgcg gattctcttg gccgtgtgca gcccaaccaa cctcacccaa ctcgacaccc
    15061 aggcggaagt cgcgcgggtc gccgaatcgc tccgcgacct cgtcgagcgt gggctggtca
    15121 tcgtggaccc gctgccgaac gcgacgctca ctgcgctgcg tcaggcgctg cgggcgaccg
    15181 actatcacgt cctgcacttc atcggtcatg gcggcttcga cccggtcgcc ggcgacggcg
    15241 tgctcgcctt cgaggacgcc gaccatctgg gccacctcgt gtccgggcag gacctgggca
    15301 cgctgctgca cgatatgcgc accctgcgct tggccgtcct caacgcctgc gaaggcgctc
    15361 ggcagtcgcc caccgacccc ttctcgggtg tcgcgcagtg tctggtccgt cagggcttgc
    15421 cggcggtcgt cgctatgcag ttcgagatca ccgataccgc agccaccagc ctgtcgtcgg
    15481 agttctatgc cgctctggcc gacgggctgc ccatcgacgc tgctcttgca catgcccgga
    15541 tgagcgtgtt cgctgcggac aacgacgtcg agtggggcac gccggtcctc tatctgcgcg
    15601 cccgggacgg gcgaatcttc gacgtggcaa ctcaaggtgc ggccccgccg acacctacgc
    15661 cgacaccagt ggcgacaccc gcgccgacac ccgcgcccgc tccaccatca cccatcccga
    15721 tcccttaccc gatctcgacc actccaccga ctccggcgcc gatgcccgta ccgacatccg
    15781 caacaccccc gccgatgccc gtgccgacgc cacctacccc gcaaacgggg gcgggcggca
    15841 tacctgctga cgcgccggga gcgccgaagc agcgccatcc cgcggtggca cccgctgtcg
    15901 tcgcagccgt catcgtcacc ttgggcctgg tcgcggcgtt cttgctgcgg cccgatggcg
    15961 gcgcggcgac gaatggaccg gggccggcgc ccaaggagtt caacgggttc gtcgcgatgc
    16021 gcggaacgcc ggtcatcgac ggccaggcca acgaatgggc cggagccgtg ccctacgtct
    16081 cggacgtcct cgtcgcaggc aaggcgacga agatcaaggc gacgtggagc ctgatgtggg
    16141 accagcaggc cttctatgtg gtcgccgatg tcgccgaccc caacctcatg ccagccgacc
    16201 agacggctcc cgaccagcag tggcgcggag attcggtgag cttcgagctc ggggctgatc
    16261 cgcggggctt ggccgtcgag gctgggctgc gggccacgga cgcgcactac atcgtcggcc
    16321 tgaccccgac gaccccggcg cggaccaaga tcgtcatcaa ccgtgccaac gtcaccaaga
    16381 acaccatcga cgccggccga ttccacgccg agagcgacgc cgtcgttgcg ctcattcccg
    16441 gcggttaccg ggtcgaggcg cgcctgccct gggccgtgac gggtgtggcg ccccttgggg
    16501 tgcagacagg tcaggtgttc gggatcaacc tcaacgccag cgacgccggg tcctcaccca
    16561 aggagttcgc cgcgatgtac tccagcaacc ggggccgcac cagtgccaac caggtccgac
    16621 ccgctgcctg gcaggtcgtg caactgcagg gctgacctgg ggaggccccg aaccccgctg
    16681 gagctaggcg tccactgttt gcgcgcggcg gctcggtgag ggaagatcta cccacgggta
    16741 accccacccg gcgacccgac tcgggaggac gacgatggcc aggtacgaca tcacgaccgc
    16801 aaccttgggc accctgctgg ccgaccccga cgtggtggcc atcatggaga agcacgcccc
    16861 cggcatcacg accaacccca tgatcggcat ggcggccggc atgcccgctg cccaggccgt
    16921 cgccatggcc ggcgggatga tcggcggaga cgccgtgtcg gcaatcaccg acgaggtcac
    16981 ttctcttaac tgaggtgcat gtagtacagt cttcacagac accaacacag acccacgagc
    17041 ggctgccgga ccccctgctg gcggccgctc gtgtttgtgt ttccggggac cattcgccca
    17101 catcggcaga gttcggtcgc ccgctcccgc gctaggcacc cccgtttggg cccgttcccc
    17161 gttgtgacgc cacccgccgc ggtaggccgc cgccacgcca cggtatgccg cgggcgcggc
    17221 ataccggata gcgtgcggtg atggccgcga aggcaacgac ccccgcgatc gagctcgctg
    17281 tcggtgagcg gacgatccgg ctcagcaacc ccgaccgggt gtatttcccg gagcacgggt
    17341 ggaccaaact cgacctggcg aactactacc tcgcggtcgg cgacggcatc gtccgggcgc
    17401 tgcgggagcg gccgtgcatg ctgcaccggt tccccgaggg gctggccggc gagaaggtcc
    17461 accagaagcg gatcccgcac ggggccccgc cgtggttgca gaccgtgcgg ctgcacttcc
    17521 cccgctggaa ccgcaccgcc gacgaactgt gcgtcaccga gctcgggtcg gtcatctggg
    17581 cggtgcagat gtcgaccgtg gagttccatc cgtggaacgt ccggcgcgcc gatgtcgatc
    17641 gccccgacga gtggcggatc gacctcgacc ccatgcccga ggccgggttc gaccgggtgc
    17701 gcgtcgtcgc cggggtcgtc cgcgaggtgc ttgacgagct gggcatcgtc ggtttcccga
    17761 agacttcggg gggcaacggg ctgcacgtct acgtccggat cgtgcccgag tgggagttcg
    17821 gcgacgtccg ccgggcggcc ctcgccttcg cccgcgaggt ggagcgacgg ctgcctggag
    17881 acgcgacgac gacctggtgg cgcaaggacc gcgacccccg gcatgtcttc atcgactaca
    17941 accagaacgc gcgcgaccac accatcgcgg cggcctactc ggtccgcggc gtccccgacg
    18001 gcaccgtttc gacgccgatc cggtgggacg agatcgccga cgtggaaccc cgatccctca
    18061 cgctcgcaac ggttcccgcg aggtatgccg ccctcggtga ccttcacgcg gggatcgacg
    18121 acactgcata ccggctggat gccttgttgg aatgggccga gcgggacgag cgtgcgggag
    18181 ccgacacgcc acccgagccg gacgccggac ccgacacctg atcgccgacg cctggacgcc
    18241 gacgcctgga cgccgacgcc ggaccctgcg gcccgcccga cgccggaacg cgccaacctg
    18301 tgaaatttcg atgaatccgc aaccaccggc gctcaggagg taaggggcgg cttaccctca
    18361 gtcatggccc cgttgccacc tcggccggca cccccggctc cggctcccga aacgccgcaa
    18421 cccgcccgcg cgcgggtcat cgcccggcgt gccctggcct ggggccgccg ctggtggatc
    18481 ctcacggtgg tcctcgtggc actcgccggc ctctcgggca ccctgctcnn nnnnnnnnnn
    18541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18601 nnnnnnnnnn nnnnnnnnnn nnnnnnnngt ggatcctcac ggtggtcctc gtggcactcg
    18661 ccggcctctc gggcaccctg ctcgtgcggg acctacgcgc ccccgcgccg ctcacccagg
    18721 ccgagatcga caaggccgcc gcgaaagtcg tcgacaaggc gctggccgaa gccgcgcggg
    18781 ccccgtcagc cgccagcacg gcatacgccc aggtcgcggc gagcctcgtc atcatcgaaa
    18841 ccgttggcgg gggaggcgat ccggctgcgc agggcctggg gacgggcgtc atcgtcagcc
    18901 aggagggcgc gatcctcacc gccaaccacg tcgtcagcgg cggtggcgcg atcacggtga
    18961 ccttcgccga cggcactcgt gcacccgctc gggtccagag cgcgcagccg gagaacgaca
    19021 tcgcggtgct cgtggccgac caactcccgc aggtcgtcgt gccagcggtc ctcggtgggg
    19081 gcgtgcgcgt cggcgacccg gtgtatgccg tcggccatcc cctcggtctc accgcgtcgc
    19141 tgtcgtccgg tgtcgtgtca gcgaccgggc gaacgatcga ggcggaaggg ctcaagctcg
    19201 ccgacctcat ccagttcgac gcggccgtca acccgggcaa ttccggtggg ccactgctca
    19261 attcggctgg tcaggtcgtc ggcatcgtca ctgcgctcgc caacccgtcc aagcaaccct
    19321 tcttcgtcgg catcggtttc gccgtgccga tcgcgacagc cggcggcgcc gccggcggac
    19381 cctcgatcta aggagccaga tgtcgactcc ctttgccgcc ggcacctctg ccgccgcccc
    19441 ctcgccgggg ccaggcgccc acccgctcga gcagatgctc taccaggtga agcggaccat
    19501 cgtcggccag gaccaactcc tcgagcggat gctcgtcgcg ctcctggccc gcgggcacct
    19561 gcttgtggag ggcgtacccg gcctggccaa gacgatggcg atcaagacgc tcgccgaggc
    19621 gatcggggtg gacttcaagc gcatccagtt cacgcccgac ctggtccccg ccgacctcat
    19681 cggcacccgg atctacaacc agcagactgg cgacttccag gtgtcgctcg gccctgtctt
    19741 cgccaacctc gtcctcgccg acgagatcaa ccgggcgccg gccaaggtgc agagcgccct
    19801 gctggaggcc atgcaggaac gccaggtgac gatcggccgc gagaccttct ccgcgccgga
    19861 cccgttcctc gtcatggcca cccagaaccc catcgagtcc gagggaacct atccgctccc
    19921 tgaggcgcag gtcgaccggt tcatgctcaa ggtgctcgtc ggatatccct ctgccacaga
    19981 ggaatacgtc gtcgtcgagc ggatgatccg cgcggccgag cggatcgacg cggtcctcga
    20041 cgcggacgga cttcgggggc tgcaggccgc cgtcgacacg gtctacgtcg accccggagt
    20101 gatccagtat gccgtcgcgc ttgcgggtgc gacccgtgac ccaggcgtcg tggggcttcc
    20161 cgaactggcc cgctatctgt ccttcggtgc cagccctcgc gcgtcgatca acatcgtgct
    20221 ggcggcccgg gcgtgggcct atctgcgtgg ccgtgattac gtcatcccga tggatgcccg
    20281 ggagcttgcg cccgatgtcc tgcgtcatcg gatggtgctc agctacgagg ccctggccga
    20341 tgacgtcagc gccgacgacc tgctcggccc gatcctcgcg gcggtgccgc tgcccgagac
    20401 tgcgggaggg cggcatacct caggggtggc cgagccgatc cccgccaacg tggaccccgc
    20461 atggtaccga cgaccggagt agctgccccc gagcggctgc tgcgccggct ggagtggacg
    20521 gtggtgcggc ggctcgacgg gcggttgcag ggcgactacc ggaccatctt ccgtggcacc
    20581 ggggtcgact tccgcgatct gcgcgaatac gagccgggcg acgacctgcg ccacatcgac
    20641 tggaacgtca ccgcgcgcac cgacgtgccg ttcgtccgcg aatacaccga ggatcgtgag
    20701 ctgaccgcct ggctgttgct cgaccggtcg gcgtccatgg ccttcgggcc gaccgatcgg
    20761 cccaagcatg tggtgctgct tgagctggcg accactctcg cactggttct cgcgcgcgga
    20821 ggcaaccggg tgggtgccgt cctctatggc gacggggtcg atcgcacggt gccgccgcgg
    20881 caggggcggg atcaggtgct gcggatcgcc cgcgagttgc tcgccgacgc cccgactgcg
    20941 gggcacccga ccgacctggg cgggttgctg cgggcggcgt tgggcatggc ccggcagcgc
    21001 tccctgatcg tcatcgtgtc ggacttcgtg acgacgccgg ggtgggagcg gccgttggga
    21061 ctgctcgccc gtcggcatga cgtcgtggcc atccagttgg tcgacccgcg tgagcatgac
    21121 ctgcccgagg ccgggctcat cgcgctcacc gaccccgaga ccggggagca tgtcctcgtc
    21181 gacaccggtg acgttgagtt ccgttctcgg ctatgggagt tggcgcaggc gcggcagcgc
    21241 caactggagg cggtcgccgc tcatgccggg atcgcgctgc accccgtgtc gaccgacgag
    21301 gatctcgtcg gggcgttgct gcggatggcc cgtctgcgca gcaagtggcg gcgctgatgc
    21361 cagtgacgtt catttccccc tgggcgctgc tcgggctcct tctcgtgccc ggtctcgccg
    21421 cggcataccg aatcgtgcga gcccgccgag cggcccggcg agccgagctc gcagcgctcg
    21481 ggttcgctcc ggtgttggcc gggggccgtc gtcgacgagg ttgggtcgtc ccgaccgcgc
    21541 tgggtctgac gctggggctg ttggtgctcg ccgccgcgcg tccgcaggcg accgtgcccg
    21601 acctgcgccg cgaggggacg gtgatcctcg cgatcgacgc ctcgacgagc atgacggcca
    21661 ccgacgtggc gccgacccgg atggcagcgg ccaaggcggc ggcgaaggcc ttcattgcgc
    21721 ggcagccgag cacgatccgg atcggggtcg tgtccttcgg ggacagcgcg gtggtcctgc
    21781 aggctccgac gctcgtgcag gccgaggtcg tggcggccat cgatcggctg acaccccaag
    21841 ggggcacgtc gttggggcag ggaatcttcg cgtcgctcaa ggcaattgct ggagggaagc
    21901 tggcgatcac cgaagagcag ttggcggggg acatcgagag tctcgacatc gggtacttcg
    21961 ggtcatcggc catcgtcctg ctgtccgacg gtgaggacac ctcgcgcctg gaccccctgg
    22021 cgttggccga gctcgccggt gtcgcgggtg tcaagatcaa caccgtcggg ctcggcgacc
    22081 cggccgggac gacggtgacc gtcaacggct tcacggcggc gacgcagttg gacgaggcga
    22141 cgctgacggc gatcgccgac gcgacgggtg ggcgctatcg ggcggcaccc gacgaggcgt
    22201 ccctcgccga tgtctacggc tcgatcgacc tcgcgatgac cagcaccccc gtgcacatcg
    22261 agatctcggc gctgttcgcg gcggccgccc tgctcgtcct ggtcgctgcc gccagcacgt
    22321 cgatcctgcg gaccgggcgg gtgatctgag tgtcgttcgc gtggccctac gccctgctcg
    22381 cggtgctgct cgctccggcg ctgctcgcgg catacctctg gtcgttgcga cgccggcgca
    22441 ggtatgccgt ccggcatccg gatctcgcgc tcgtgcgtgc cgcgctgccc gcgcgttccc
    22501 gctggcgtcc gcatctgccg gttgcgctgc tcgtgctggg cgtcgggctg ttggggctgg
    22561 cagtcgcccg gccgcaggcc tcggtcgagg tcccgatcca gcgctcgacg atcatcctcg
    22621 cgctggacgt gtcgcgctcg atgtgtgcca ccgacgtcgc gcccaaccgg ctttcggccg
    22681 cgcggacggc cgtccaggag ttcgtcgaga cccagccgag ggacactcgg ttcgggctgg
    22741 tcgcgttctc gggatcggct gcactcgtcg tgccgccgac gaccgactcg gcggcggtgc
    22801 tgacgtcgct cgacgggctg agcaccgggc gcgggacggc gatcgggtcg gccatcctca
    22861 cgtcggtcga tgcgatcgcc gcgatcaact cccaggtgca accgatcggc gaggtgccgc
    22921 tcgggcccga caccatgcag cagccgggcg tcccggcgcc ggtgccgagt tcgatcgacc
    22981 cgccgcctgg agcgactggc ggcttcgtgt cggacatcgt cgtgctgctg accgacggcg
    23041 cgaacacgcg tggggtgctg ccggtggcgg cggccaaact cgctgccgag cgacgcgtcc
    23101 gggtctatcc catcggtttc ggcacgacga atccgaccca gatggtgtgc accgccgagc
    23161 agttgggcgg ggacgctttc ggtgagttcg ggccacgatc tggcggacag gtcccgggtg
    23221 gacaggcccc gggtggacag gccccgggcg gcgggttcgg cggagggcgc aacttcctgg
    23281 tcgtcgatga gcccaccctg aaggccgtcg cggccaccac cggcggggcc taccaccagg
    23341 cgagcgatgc cgctcagctc ggcgaggtcc tgcgagggat cccgcgcgag gtcgtgctgc
    23401 agcgtgagga cctggaggtc acggcatact tggcgatcct ggcagcgctc gtcgtggcgg
    23461 gcgcgctcgt gctgtcggtt cggtggaacc cgctcgcgta ggttcctggc ctcggccggg
    23521 gttgaggtct gcctggcccc gcctgtggtt gaggtgttga cccgctcgcg tggctgcctg
    23581 gccccggctg ggtttaggtg tggcggaacg ggggccaggg accgcgttcc accacaccta
    23641 acgggaggtg tggtggtgtc gccatcgagc gtgaaggtgc ctgcccagtc aagtacgacc
    23701 ggtcaacaaa ctctgacacg gtgtcacgct ctacccttcc gctagcgacc gcgggaaccg
    23761 accggggtgc gcctgcgagc accgacgcgg gatcgaggga cgggggccat ggacaggttc
    23821 gagctcaaga aggactatcc gtcgctctat gcccccagat cgcgcacctg ggaagtgctc
    23881 gatgttcccc ccatgctggc gatcgcggtg gacggagccg gtgacccgaa catgtcaccc
    23941 gactatgtcg aagcggtcag tgccctcttc tcggtcgcct acacgatcaa gttcgcggcc
    24001 aaggcacagg gacgcgagat ggtcgtcggg ccactcgagg gtctgtggtg ggcagaggat
    24061 ccccgcagtt tcatcgaacg cgacaagggc gcctggagtt ggcggatgct catcgtccag
    24121 ccgccgtggg tcgacgaggt gaccctggag gcagcggtgg ccaaggttgc cgccaagcag
    24181 gccgtcaaag gggccggctt gggcagcaca gcgctgtcga agctgacgct ggtcaccctc
    24241 aacgggggcc gctgcgctca ggtgttgcac atcggttcct atgacgacga gggccccacg
    24301 ctggccgatc tgcacgaccg cttcatgcct gaacacgggc tggcgttcaa cgggccacac
    24361 cacgagatct acgtcggtga cccgcgtcgt acggcaccgg agaagctcaa gaccgtcctg
    24421 cgccaacccg tccgcccggc ctgacgcacg ccggtccccg agatcatcgg cgggtgtggg
    24481 gaattcgggg tcgctggagc cggggtgggc cgaacttgcc ggcgagtgtg tggaatttgg
    24541 ggagcgggga ggg
//
LOCUS       DAOMZY010000011        24373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_1739_length_24373_cov_5.667900, whole genome shotgun sequence.
ACCESSION   DAOMZY010000011 DAOMZY010000000
VERSION     DAOMZY010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 24373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24373
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1739_length_24373_cov_5.667900"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..886
                     /gene="metF"
                     /locus_tag="PLK64_01430"
     CDS             5..886
                     /gene="metF"
                     /locus_tag="PLK64_01430"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HPV78430.1"
                     /translation="MRSIPDALAQGRPSISFEFFPPKDDAGEAALWEAIRDLEKTRPT
                     FVSVTYGAGGSGRDRTIRMTDRIAQETTLTPMAHLTCVGHSVAELRSIIGHYVNVGVR
                     NVLALRGDPQGGLDQTWERHPDGLDHADELVSLLRTLGPFTVGVAAFPDRHPESPSLD
                     HDADVLVRKADAGADFAITQFFFTREAYVDLRDRVVARGRDLPIIPGLMPVTNVRQLA
                     RMTQLSGQPVPREVTERLERVADDPVAVRAVGVEIATELAQGLLDEGAPGIHFITMNR
                     STATLQVWGNLGLEAAQ"
     gene            complement(916..1596)
                     /locus_tag="PLK64_01435"
     CDS             complement(916..1596)
                     /locus_tag="PLK64_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HPV78431.1"
                     /translation="MGAEILPWVFTSGWASGINAYAVVLIMGLAEKFWQVQGIPDALG
                     RTDVLIGAGVLFVIEMVADKIPYLDSTWDSIHTVVRPAVGATLGYLLGNESADLNAAF
                     TAATGGILALLSHLVKAGTRAAVNTSPEPASNVVLSTAEDVTVAAVVTTAFANPWLAA
                     SIAGLLLVVGMVTVLFLLKRIRRFRARYNAWGDRLGSSEPKADGSLRDAPARRGDTAR
                     LPQGPGAD"
     gene            2180..2644
                     /locus_tag="PLK64_01440"
     CDS             2180..2644
                     /locus_tag="PLK64_01440"
                     /inference="COORDINATES: protein motif:HMM:NF039800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HPV78432.1"
                     /translation="MVRRYAEPVRVGTASRLTPDSLADLEQVPPGPGGYAASSPTWFE
                     WAGRRYHVRAILAHWRERRAWWREVLDADPTLEATSSGPASNTASTPASNTSSDTDCR
                     SAIAVAARERLVWRVEAGPGWLTRTGVFDLACDETSPGDPDPDGWLLLRVSD"
     gene            2712..3209
                     /locus_tag="PLK64_01445"
     CDS             2712..3209
                     /locus_tag="PLK64_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV_6107 family HEPN domain-containing protein"
                     /protein_id="HPV78433.1"
                     /translation="MSAAPSHPYPATPSPPLRPRRHVPPSATVMELLDRAQASLLYAS
                     QADQVGERYVLAHLAGLRAAAAVLAARTTPARSSRPRSVWEVLPTLAPELTEWAVFFA
                     ASARQRAAVERGDRVLPAREADDLVRQAEAFIEVVQDALGVPRTFVRPDYLAPVTPLA
                     RHAGR"
     assembly_gap    3410..3509
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3510..7648
                     /locus_tag="PLK64_01450"
     CDS             <3510..7648
                     /locus_tag="PLK64_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78434.1"
                     /translation="SASTSSTAPPVWARPSAPVRTPVRGGAIVDPRLPRVTVLARGLA
                     GGVAPGLGWARLCRLVTRTHLSGERGNPLTTPELLAELAAPVGDDPAALIVLLGPDSD
                     VGRAVLAKRRAAAYQALERWRAVLPAGALALETVCHGGPEGTPASLGHAARMLALGRD
                     LGVPVILSAQVRHADPAEAATVDVLDAARRLVALDVRHLDRVTTAGHLAPTPAMYAVA
                     CDIVQASGPGLAGGTSTVRDGARDLLAATVALASECVLDPAIDLGIGGVHLPEQSTLG
                     LGLHDDPQHHLVQRCRGAVGARYPGATDGEVARISARLDEELEVIAALGYPTYFLTVA
                     TVTDLIRDMGVRVAARGSGAGSLVNYLLGISGVDPIRYDLLMERFCSPLRAQLPDIDI
                     DVESARRTEIYEKILARFGGDRVTCVSMMDSYRVRHAIRDVGAALGLPPGEVDAMAKA
                     FPHISARHARSAIADLPELRVSGLGAARYDLMWDLVERLDGLPRHIALHPCGVILSNA
                     ALLDRTPVEASWIGFPMSQFDKDDVEVMGLLKLDVLGIRMQSAMALAVDEVARVDGIP
                     VDLDDRAQVPLDDEATFAMIRTTRTLGCFQIESPGQRELIGKFGPERFEDLVIDISLF
                     RPGPVKSDMIVPFLKARHGWAEPEYLHPTLIPALAETAGVVVFHEQVLRIVAETTGVS
                     LAQADEVRRALGSPQGQLDVEAWWRPAALARGYTVADADRIWEVLRAFASFGFCKAHA
                     AAFALPTYQSAWLKTHHPAAFLAGVLTHDPGMYPKRLILDDARSLGIEILGLDVNASK
                     SWYAVERLPGSAPGSALGRGISPSSPDGGIPRPGTDPDLPDGRGYGIRVSLTDVKGIT
                     EAEVTRIVAGQPFTDLADLAHRAQVSRPIMERLVLAGGLDSVYGISTATGAGADRSFG
                     AGGLGVRERVTRRDLLLHVAELDRWVRSGGSGQGKALRGGRSRFTRRPATMSATGPGG
                     EAAGAPVTDTRALAAAHPRREVAVLVQQPGVPAGPGTIRAFDVAALAAAQSQAAAPVH
                     VSDRHTQMVLPLGDTPTLTRGSGLPELSGPERVRAELEILGLDASAHVVAHYEPMLEA
                     LGVTRSRDLLSARNRSEVWVAGVKVATQTPPIRSGRRVVFLTLDDGTGPSDSTFFEDV
                     QGPYAATVFHSWLLLVRGVVRRTGARGISLRATGAWELSGLWEAFATGGRPRLLDALA
                     AADAAAHDRAAAAAAAYALPYAVPRGTGWAEPAADAAAAAADGSPVGDRDDTAYADRE
                     RAARLDHAASLVGGRPASRAGGMGGGSPTSPVGAPGAPRQVLVHASGFKQSPYADTRP
                     PGEDVKSARHLAGAVDHAGLTDQVTPDAFDALDAAGQGSGAGRPPRKLWHSSPGSSGH
                     "
     gene            7658..8443
                     /locus_tag="PLK64_01455"
     CDS             7658..8443
                     /locus_tag="PLK64_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPV78435.1"
                     /translation="MSEEQQRRARRAATGVRTAALWAAVKDLVTERAAALGRPLRVLD
                     LGGGTGELAVPLAELGPDLVADVTVVDPSPDALAALGRRAAESGVQARVHGSQGDADT
                     LADLLPERAFDLVCCHGVLEVVDDPTATLRVLADALADDGHLSLLVAGRLATVWARAL
                     AGEFGQAHTALTSPDGRWGAHDPLPRRFDLAQLRALVESAGLQVEHWHGVRLLADLVP
                     STSIDTDAERAALVALEEALAGHPAYPFLGELGAALHLLARRG"
     gene            8465..9724
                     /gene="dinB"
                     /locus_tag="PLK64_01460"
     CDS             8465..9724
                     /gene="dinB"
                     /locus_tag="PLK64_01460"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPV78436.1"
                     /translation="MSRRQFRPPPRTGDGPPDDTGCTVLHVDMDAFYASASLIDRPEL
                     RGTPVIIGGGGRSVVLSATYEARAFGVTSAMPMTRARRLCPQATVIAPNHDRYAAISD
                     AVMATFASVTDRYEPLSLDEAFLDIAGAVRRLGPPTSIAQWLRDTIADEQSITCSVGV
                     ASTKFVAKLASGLAKPDGLLVVPQDEVLAFLHQLPVGALWGVGDRTEEALQRLGLHTV
                     ADLAHTPVDTLRRALGDATGTHLHALAWGRDERRVLPQQREKSVGSDETFAQDIDDPV
                     LIHRELLRLADRTAARVRSAGLMGRTVTLKVRFADFTTITRSRTLRDPTDVSREIYAA
                     ATALFDALGLQRARIRLVGVRMEGLVESERAAVQPRLDDPDFGWRDADRAVDRASARF
                     GAGAVRPAALVRGESVAHPGSMPRGGE"
     gene            9894..10286
                     /locus_tag="PLK64_01465"
     CDS             9894..10286
                     /locus_tag="PLK64_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HPV78437.1"
                     /translation="MALSEREQKLLEQMEQALYAEDPRFATRIRSHGDRGTKRRMLVG
                     ILAVLAGLGLVFLGVLGQAIWLGAVGFAVMVAGGVWAFAPARKTGPVGLVQGDGSTAA
                     RTAKARKSGQGFMNMLEERWERRDRDQW"
     gene            complement(10338..10841)
                     /locus_tag="PLK64_01470"
     CDS             complement(10338..10841)
                     /locus_tag="PLK64_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HPV78438.1"
                     /translation="MSFADPVSLTIVTGLLMVVGLVGIVVPVVPGLLLTVAATLLWAY
                     AHPAPGAWVVFWVAVVLYAAGVVTQYLLPGRKMRREGVGTSTMLLAVLLGIVGFFVIP
                     VVGALVGFVLGIFLVELTRRQDRSAAWTSTKAALVAVLHSMGIELLAGLAILLTWIIG
                     LIVLGPG"
     gene            complement(10838..11059)
                     /locus_tag="PLK64_01475"
     CDS             complement(10838..11059)
                     /locus_tag="PLK64_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78439.1"
                     /translation="MNATLVHRLSAADRCDRCGARAYIRARLHSGGELYFCAHHGRQH
                     LPQLADQAVEIHDETELLTETADESTGLR"
     gene            11323..13488
                     /locus_tag="PLK64_01480"
     CDS             11323..13488
                     /locus_tag="PLK64_01480"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HPV78440.1"
                     /translation="MATRSTTGRTAASGSGAGGGDYTARHLQVLEGLEAVRKRPGMYI
                     GSTDQRGLMHCLWEIIDNAVDEALAGVGDRIEIILGGDGSIEVRDNGRGIPVDTEPRS
                     GLSGVELVFTKLHAGGKFGGGSYAASGGLHGVGASVVNALSSRLDVEVDRGGKTYAMS
                     FRRGEPGVFADPPGPHSPEAPFTAYETGSELRVVGKAPRGATGSRIRFWPDLQIFLKE
                     ARLDDDLLAARARQTSFLVPGLALVVRDERGAPDTPGAQGTHEQVFQHLGGISEFAEF
                     LAPDPPLTAVWRLQGTGNFTETVPVLDERGHMSPTEVERECSVDIAMRWGTGYDSRVS
                     SFVNIIATPKGGTHVAGFEQSLLKVFRRQLELNARRLKVGTDKVEKDDILAGLTAVVT
                     VRLAEPQFEGQTKEVLGTAAVRAVVAKVLEQELNARLTSTKRDDKAHAALLLEKVVSE
                     MKARISARQHKETQRRKTALESSTLPAKLADCRTHDVLRSELFIVEGDSALGTAKLAR
                     SSDYQALLPIRGKILNVQKASVADVLKNTECAAIIQVIGAGSGRSFDLEAARYGKVII
                     MSDADVDGAHIRTLLLTLFFKYMRPLVAAGRVYAAMPPLHRLEVIGGPGRKNEYVYTY
                     NEAQMRSTRAALERKGRKVKEPPQRYKGLGEMDADQLADTTMDPRFRTLRRVTLGEAE
                     EAARIAEGVFDLLMGNDVAPRKDFIVESAKTLDRERIDA"
     gene            13622..14689
                     /locus_tag="PLK64_01485"
     CDS             13622..14689
                     /locus_tag="PLK64_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018761549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPV78441.1"
                     /translation="MSTTIDPGPPPGAQAAYPTSASTTPRPGVTAIPPEDLAWARSTV
                     ATISRSFQERVVGQVRLHETLLLGLLTGGHILLESVPGLAKTTAAATLARTVGGSFKR
                     IQCTPDLLPSDIVGTQVYDVRTTQFETRLGPVHANLVLLDEINRSSAKTQSAMLEAMQ
                     ERQTSIGGQVYPLPEPFLVLATQNPIEQEGTYVLPEAQLDRFMLKDVLDYPTLDEEAE
                     VVRRIDAGIYLAEQTPAVSLADVRRLQALTRAVYLDDTVIRYAVGLAYVTRHAERYLD
                     PALARYVEFGASPRASIAFAQAARARALLAGRDHAVPDDVQALAHRVLRHRIILGFEA
                     VADDVPVERVIDAIITAVRTP"
     gene            14742..15626
                     /locus_tag="PLK64_01490"
     CDS             14742..15626
                     /locus_tag="PLK64_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018769444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPV78442.1"
                     /translation="MAALLTRVKSKLFIVAHRRTWGLLDGEYASVFRGRSLDYDDLRE
                     YIPGDEIRDIDWKATARHGAPLVKRYVATRKQTVLLLVDTGRSMAAMAPSGEPKKDIA
                     IQIAGMLGYLAVRHGDLVGLISGDGESTRMLEPAAGEANLERLLRVIDRASTLEAPPS
                     RFSAQLHYLAEHVRRRMLIVAVTDDGGLGSDDDPLLRRLHAQHEVLWVVVADADPTRF
                     DPRADASEVTDGSALPGAVRGGERLQAAYQQALAGRREATDQRLRRLGIARARVGGTS
                     EVVGSMFALLERQRRRAR"
     gene            15616..16098
                     /locus_tag="PLK64_01495"
     CDS             15616..16098
                     /locus_tag="PLK64_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78443.1"
                     /translation="MHVDDPFYPPMTYHWVWTLLAWVAILAVLAWAGFIWWRTRRVRP
                     TTPAGPVPIHPGWRLARAKHDTMTRIDQIVWECEQGRVDARTSHRELSAAVRAFIDDV
                     SGATTSRMTLSDLGSRGPGLAPVTHVVLQLYPGEFGPDPARPIRPAAEAAKAVVAQWH
                     "
     gene            16089..17060
                     /locus_tag="PLK64_01500"
     CDS             16089..17060
                     /locus_tag="PLK64_01500"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPV78444.1"
                     /translation="MALKQPWILPWAALGALAIAALVWFALRRRRPPAALPLANTDLL
                     TSLPEYVHAIWRHRLMTVLYAAAAGFVAVLAIIGAARPVATTVINPEKANRDIVLCLD
                     VSGSMTTADAEVAETFRSLAKEFKGERLSLVIFNSSAVPVFPLTDDYDFVEEQLSDAQ
                     RVFEGSFDHEFYAGTLNGQGSSLIGDGLATCVRSFDHGNLTRARSIILATDNLAAGRS
                     IFSLAQGGTMAKAAGIQIYGLNPAHRAGSSTATEMRSVAEGSGGLYYPLNSVDAVSRI
                     VSAVSAREASRIPGAPITLVHDQPASWVGWTWLGVLGLLGALRRWRR"
     gene            17057..18121
                     /locus_tag="PLK64_01505"
     CDS             17057..18121
                     /locus_tag="PLK64_01505"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPV78445.1"
                     /translation="MTLLPVMSVGVLVLAVVVALALVWWPQPGPQPSLRTRVRRTLLV
                     AAVLVASLRPGLPGAEVRASASELNVFFVVDTTTSSMAEDFGTDPRMSGMRADITAIS
                     GRMAGARFALITFDQAAVLRMPLTSDGAAVAAAAETMLPETSAWSRGSSVTAAGPLLQ
                     QTLARVAAAHPERARAVFYLGDGEQTSGSPPAALGVDQSLVNGGAVLGYGTTGGGQMH
                     ETGVPGSRADAGWLLDPSTGKPAVSVIDEGQLGSIAQQLGVPYLHRSPGESVDAVLGL
                     VDLTGLDRVTVAQDGPLSAGREEFYWIALLAVAGLVAWEVGLALARLWGLRRPRRARG
                     IPEDGGIPADRDGHEEKVRS"
     gene            18118..18894
                     /locus_tag="PLK64_01510"
     CDS             18118..18894
                     /locus_tag="PLK64_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78446.1"
                     /translation="MTLAPHPAPARPAALDPDEPAAARHARLAMRRRLISWSIGPAFV
                     AILVAVKLLTMSAAATQAVDGFFAAAAHTVFEAADRMSFLNVVERHKAPFARGDAFVL
                     AGDLDSARAQFEQALELAPPDSIDSCQIRVNLALALEKLGDAAKAAGDEVAAKDYWTR
                     TKAVADAAPPGCFPPAADGAGQKAEAAGDRAEQKLNPQQSSGVDQSSDAQQQAEQQRK
                     ADELDQQTHENQQDRQQNGPDGQGNPAGGGGSANPAAKPW"
     gene            complement(18950..19972)
                     /locus_tag="PLK64_01515"
     CDS             complement(18950..19972)
                     /locus_tag="PLK64_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPV78447.1"
                     /translation="MQQPTIADVARAAGVSVATVSRALRGLDKVHPETRERVVQAAAQ
                     LDYIASPTASGLASGRSRLIGIITPFMARWFFTGIMSAIEKTLREHQHHILLMDLERS
                     ETSMSRLSLTQGMLFKRVDGLIVINVELQDAERDLVRRLGLPVVAVGSPFEDSPCVGI
                     DDIGSAALAAEHLLALGHRKIAYVGRDYPEAAPRKTPIDRLEGFHQAMSRAGINTPPT
                     WVLDSDWTAGDSRASAMALLSGPDRPTAVLAASDEMALGVWGAARQLGLEVPRDLSIV
                     GIDDHELAPVFGLTTVRQDVAAQGVAAASTLLALLGLYDPSAIPDQTFPVELVIRGST
                     TAPAKP"
     gene            complement(20189..22384)
                     /locus_tag="PLK64_01520"
     CDS             complement(20189..22384)
                     /locus_tag="PLK64_01520"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HPV78448.1"
                     /translation="MTTAPSVPTMLHLRLGGTSVVARLDDAALPCVLHWGPDLGDVST
                     QALDELQIALTPPYVDSPISSQAAVALLPQHSSGWLGRPGLLGSRAGGRSWSVSFDRV
                     EHRVETAGSADWADGAAGAVRLRSVGLDRPSALEVTTEIEVHPSGLVRVRAQVRNLGA
                     DDYEVAHLLPALPVPAEAGELLDMSGRHTHERTPQRRPFDPGAWVREAWGGRPGHDSA
                     TVMCDGEPGFGFRSGRVWGVHVAHSGNQVVLAEHSTAGWRLLRGGEVLLPGEGVLHPQ
                     ESYSSPWLVASWGEGLDAFSQRVHRFLRARPHHPRGPRPVLLNTWEAVYFDHDLGKLI
                     TLADRAAELGIERFVLDDGWFRHRRSDQAGLGDWFVDEGVWPDGLHPLVDHVHGLGMD
                     FGLWFEPEMINLDSDLAREHPDWVFQTDHGPGLPSRYQHVLDLGHPAAYAFVLERMST
                     LVGEYRIAFIKWDHNRPLVDAGHSPDGSPGVHIHTEAVYRLMAELKARHPGLEIESCC
                     GGGGRVDLGIIEHTDRVWVSDCIDAHERQRLVRWTGLLLPPELLGTHVGSGADHSTHR
                     LLHLGFRAGTAMWGHLGVEWDLTTASSADLDTLAAWIAFHKEVRELLHTGDVVHADLP
                     NPALLLEGVVATDRSDALYRLAALDLTVTWPAGRVRLPGLDPARDYHVSAQAPGDAAA
                     ATFPPGWGPSGVTLPGRVLREVGIQAPLLNPDQLVLLRARALPTAAVPV"
     gene            complement(22494..23447)
                     /locus_tag="PLK64_01525"
     CDS             complement(22494..23447)
                     /locus_tag="PLK64_01525"
                     /inference="COORDINATES: protein motif:HMM:NF018677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HPV78449.1"
                     /translation="MTIVDPACSRVWDTGALPAWGTAARADFVVALEQNGPWGRVAAT
                     QSHLDPALGAALDAHVSGLGGRLLLIRRPGRHAEQAPQTDAEPEARGAGAGAGADGAR
                     PQQSTRRTYIARTGPGGWLVRADLTDPAMLLGLGADELASAGATADRLGGALDTVPLL
                     LVCTNGRRDACCAQRGRVVTSAFVSGGFATAAPDQVWETSHTGGHRFAPTAVLLPWGR
                     VLARLDLELAAGALDAAASGRLAPALLGRVHDRGPMHLPPAEQAADAWLRRQTDRRDL
                     DDPMDDLREQVDVVARQGPMLPESCGKAAVPTIVYAPSWRA"
     gene            complement(23491..>24373)
                     /locus_tag="PLK64_01530"
     CDS             complement(23491..>24373)
                     /locus_tag="PLK64_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 66 protein"
                     /protein_id="HPV78450.1"
                     /translation="VALRTDGTRVDLTESFVHLIESVRERVPGARLVFNNVNDFPTWA
                     SAQAPQDAVYIEVWEPHSTHAALAQVIGRARSVAGGRPVVIAAYQQVYAVAPREEADR
                     ATALTMATVFSHGATHLLAGEADRILVDPYYVRNHVAEPGTVDLLARYYDFLTEHHDL
                     LIPSGIVEVTGSYAASYNDDLDVSYAAATVSGTARPGVVWRRITAVGDALVVHLINLS
                     GATDCRWDAPQGPPGTPGAGTLRMRRVGPDVPAVYAADPDASGHLEPLVVAVDGTHAT
                     VHLPPLATWQMIVVRSR"
BASE COUNT         3548 a         8656 c         8497 g         3572 t
ORIGIN      
        1 cctcgtgcgc tcgatcccgg acgcgctcgc gcaaggtcgg ccctcgatca gcttcgagtt
       61 cttcccgccg aaggacgacg cgggggaggc cgccctctgg gaggcgatcc gcgacctgga
      121 gaagacccgc ccgacattcg tctcggtgac ctatggggcc gggggatcgg ggcgtgatcg
      181 gaccatccgg atgaccgacc ggatcgccca ggagacgacg ctgacgccga tggcgcacct
      241 cacgtgtgtc gggcactcgg tggcggagtt gcgctcgatc atcgggcact acgtcaacgt
      301 cggcgtgcgc aatgtgctgg cgctgcgcgg cgacccgcag ggcgggttgg accagacctg
      361 ggagcggcac ccggacggac tcgatcacgc tgatgagttg gtgtcgctgc tgcgcaccct
      421 cggcccgttc accgtgggag tggcagcatt ccccgatcgg caccccgaga gcccgagcct
      481 ggaccacgac gccgacgtgc tcgtgcgcaa ggccgacgcg ggcgccgact tcgccatcac
      541 ccagttcttc ttcacacgcg aggcgtatgt cgacctgcgt gaccgggtgg tggcgcgtgg
      601 ccgtgacctg ccgatcatcc cggggcttat gccggtgacc aacgtccgcc aactggcccg
      661 gatgacgcag ttgtccggtc agccggtgcc gcgtgaggtg accgagcgtc tcgagcgggt
      721 ggccgacgat ccggtcgcgg tgcgggccgt cggagtggag atcgccaccg agttggccca
      781 agggctgctc gacgagggcg cgccgggcat ccacttcatc acgatgaacc gctcgacggc
      841 gaccctgcag gtctggggga acctcggcct ggaggccgcg cagtagtcgg tcagtcggtt
      901 tagccggcca gtcggtcagt cggctccggg gccttggggg agacgggcgg tatcgccgcg
      961 tcgggccggt gcgtctcgca gcgaaccgtc ggccttgggc tcgctcgagc ccaggcggtc
     1021 gccccaggcg ttgtagcggg cccggaaccg ccggatccgt ttgagcagga acaacaccgt
     1081 caccatgccg accacgagca gcaggccggc gatcgaggcc gccagccacg ggttggcgaa
     1141 agccgtggtc acgacggcgg ccacggtgac gtcttcggcg gtcgagagga cgacgttgct
     1201 ggccggctcg ggggaggtgt tgacggcggc gcgggtcccg gccttcacca ggtgggacag
     1261 cagggccagg atgccaccgg tcgccgcagt gaaagcggcg ttgaggtcgg cgctctcgtt
     1321 gcccagcagg tagcccaggg tcgcgccgac ggcggggcgc acgacggtgt ggatcgagtc
     1381 ccacgttgag tcgaggtagg ggatcttgtc tgccaccatc tcgatgacga acaacactcc
     1441 ggcaccgatg aggacgtcgg tgcgcccgag agcgtccggg atcccttgga cctgccagaa
     1501 cttctcggcc aaccccatga tgagcacgac ggcatacgcg ttgatgccgc tggcccagcc
     1561 cgacgtgaag acccagggca gaatctctgc acccatggtg gtgtgtgtcc ctcccactga
     1621 tgacggagtc aactgctccg tccgagggtt caacgtcgtg cagcggccgg ggtttcccga
     1681 acccgggcaa cggactgata acggtccgga ggcacataac gacccggccg cacttcaggt
     1741 caccccctgc agacctgatg cgcggccgga cgtcggtgcc cttcgttgca cgctcactat
     1801 cgcagaccga ctcaagtgtc ggcatcagta cggatactta tttgcgccgt caggcgacgg
     1861 tggccgcgcg cccagcgctg atccggatca attcggcgaa cgtcgtcgga aacacggtat
     1921 ggggtgtccg gctgccgccc agacgacgtc gtgggccgcc agcgccgagt cgacgagcgt
     1981 ggtcaggggg cggctcgtgg cggccttgac aggctgtcgg tgggggaggt tagagtgttt
     2041 ctcgaacacc tgttcggaag tggcgttggg gtcgagggcc cgcttccgac ctgacagccg
     2101 tggatgggtc gagtccgcag tgtttcgctc gcacattctc acccgcacgt ttctcacctg
     2161 ccgaagaagg ggtcttgtcg tggtgcgccg atatgccgag ccggtccgtg tcgggaccgc
     2221 gtcgcggctg acccctgact cgctcgccga cctcgagcag gtgcctcccg gcccgggagg
     2281 gtatgccgcg tcgtcgccga cgtggttcga gtgggcgggc cgtcgctatc acgtccgcgc
     2341 gatcctggcc cactggcgcg agcgtcgggc ctggtggcgg gaggtgctcg acgccgaccc
     2401 cacgctcgag gccacttcgt cgggcccggc ctcaaacacg gcatcgaccc ctgcctcaaa
     2461 cactagctcg gacaccgact gccggtcggc catcgccgtc gctgctcgcg agcgtctcgt
     2521 ctggcgggtc gaggccggcc cggggtggct cacccgcacc ggcgtcttcg acctggcctg
     2581 tgacgagacc agcccgggcg accccgaccc agatggctgg ctgctgctgc gggtgagcga
     2641 ctagtgcgcg cggcataccg aggaatcgcg gcataccgac cgatgacgag ccgaccgaag
     2701 gagaaccgga catgagcgca gcaccgtcgc atccctaccc ggccacgccg agcccaccgt
     2761 tgcgacccag acggcacgtc ccgccgtcgg cgaccgtcat ggagttgctc gaccgggccc
     2821 aggcgagcct gctgtatgcg agccaggccg accaggtggg ggagcgctat gtcctggctc
     2881 acctggccgg gttgcgggcg gccgctgcgg tgctggccgc ccggaccacc ccggcgcgct
     2941 cctcccggcc acgcagcgtg tgggaggtgc tgccgacgct ggcccccgag ctgaccgagt
     3001 gggcggtctt cttcgccgcc tcggcccggc agcgggcggc cgtcgagcgc ggcgaccggg
     3061 tcctgccggc tcgggaggcc gacgacctgg tgcgccaggc cgaggccttc atcgaggtcg
     3121 tccaggacgc cctgggcgtc ccgaggacct tcgtgcgccc ggactacctc gcccccgtga
     3181 cgccgctcgc gcggcatgcc ggccgttgac ctagcccctg ctccttcgtc tgccacccgc
     3241 gtcctgccat gtcgaccttc gtccatctcc acgtcgcctc gggctattcc atgcagtacg
     3301 gcgcctcgac ccccgcagcc ctcgtcgccc gggcggcggc cttcgggcaa ccggcgctcg
     3361 ccctcaccga ccgcgacggg ctctatggcg cggtgcggtt cgtccaggcn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ctccgcgtcc acgtcgtcaa ccgcgccacc
     3541 ggtctgggcc cgcccgtcgg cgccggtccg gacccccgtg cgcggcggcg cgatcgtcga
     3601 cccccggctg ccccgggtga ccgtgctggc ccgcggcctg gccgggggag tcgccccggg
     3661 gctgggttgg gcccggctct gtcgcctggt cacccgcacc cacttgtccg gtgaacgggg
     3721 caacccgctg acgaccccgg agttgctcgc cgagttggcg gccccggtcg gcgacgaccc
     3781 ggcggccctg atcgtcctgc tcggacccga ctccgacgtc ggtcgcgcgg tcctggccaa
     3841 gcggcgcgcg gcggcatacc aggcgctgga gcgatggcgc gcggtcctgc cggccggcgc
     3901 cctggccctg gagaccgtgt gccacggtgg tccggagggg accccggcga gcctcgggca
     3961 tgccgcccgg atgcttgccc tcggccgcga cctcggcgtc cccgtcatcc tctcggcgca
     4021 ggtccgccat gccgacccgg ccgaggcggc gaccgtcgat gtcctcgatg ccgcccgccg
     4081 cctggtcgcc ctcgatgtgc gacacctcga ccgggtcacc accgcgggcc acctggcccc
     4141 cacccccgcg atgtatgccg tcgcctgcga catcgtccag gcctccggac cggggctggc
     4201 cgggggcacg agcacggtcc gcgacggcgc ccgcgacctg ctcgccgcca ccgtcgccct
     4261 ggccagcgag tgcgtcctgg acccggcgat cgacctgggg atcggcgggg tgcacctgcc
     4321 cgagcagagc accctgggtc tgggcctgca cgacgacccg cagcaccacc tcgtgcagcg
     4381 ctgccgaggg gccgtcggcg ctcgctatcc cggggccacc gacggcgagg tggctcggat
     4441 ctccgcccgg ctcgacgagg agctggaggt catcgcggcg ttgggttacc cgacctactt
     4501 ccttaccgtc gccaccgtta ccgacctcat ccgcgacatg ggggtgcggg tcgcggcccg
     4561 cggctcgggg gccggcagcc tggtcaacta cctgctgggc atctccgggg tcgacccgat
     4621 ccgctacgac ctgctcatgg agcggttctg ctcgccgttg cgagcccaac tgcccgacat
     4681 cgacatcgac gtcgagtcgg cccggcgcac cgagatctac gaaaagatcc tcgcccggtt
     4741 cggcggcgac cgggtgacct gtgtgtcgat gatggacagc tatcgcgtgc gccacgcgat
     4801 ccgcgacgtc ggggcggcgc tgggcctgcc gcccggcgag gtcgacgcga tggccaaggc
     4861 gttcccgcac atctcggccc ggcacgcgcg gtcggcgatc gccgacctgc ccgagttgcg
     4921 ggtctccggg ctcggggcgg cccgctacga cctcatgtgg gacctcgtcg agcgcctcga
     4981 cgggctgccc cggcacatcg ccctgcaccc gtgcggggtg atcctgtcca acgccgccct
     5041 gctggaccgg accccggtcg aggccagctg gatcggcttc ccgatgagtc agttcgacaa
     5101 ggacgacgtc gaggtcatgg ggctgctcaa gctcgacgtc ctcgggatcc ggatgcagtc
     5161 ggcgatggcc ctagccgtcg acgaggtggc gcgggtggac ggcataccgg tggatctgga
     5221 cgaccgtgcc caggtgcccc tggacgacga ggcgaccttc gcgatgatcc gcaccacgcg
     5281 caccctcggg tgtttccaga tcgagagccc ggggcagcgc gagctcatcg gtaagttcgg
     5341 gccggagcgg ttcgaggacc tcgtcatcga catctcgctc ttccgtcctg ggccggtgaa
     5401 gtccgacatg atcgtgccgt tcctcaaggc ccggcatggg tgggccgagc cggaatacct
     5461 gcaccccacg ctcatcccgg cgctggccga gactgccggc gtcgtcgtct tccacgagca
     5521 ggtgctgcgg atcgtcgccg agacgacggg agtgagcctg gcccaggccg acgaggtccg
     5581 ccgtgcgctc ggctcacctc aaggtcagct cgacgtcgag gcctggtggc ggccggcggc
     5641 gctcgctcgg ggttatacgg tggccgacgc cgaccggatc tgggaggtgc tgcgcgcctt
     5701 cgcgtcgttt ggcttctgca aggcgcatgc ggcggcgttc gcgctgccga cctatcaatc
     5761 ggcttggctc aagacccacc acccggcggc cttcctcgcc ggggtgctca cccacgaccc
     5821 cgggatgtat cccaagcggc tcatcctcga cgacgcccgc tccctgggta tcgagatcct
     5881 cggcctggac gtcaacgcca gcaagagttg gtatgccgtg gagcgcctgc cgggttctgc
     5941 gccgggttct gcgctgggtc gaggtatttc gccatcgtct cctgacggcg gaatacctcg
     6001 acccggcacg gacccggatc tgcctgatgg gcgggggtac ggcatccggg tgtccctcac
     6061 cgacgtcaaa ggcatcaccg aggccgaggt gacccggatc gtcgccggcc agccgttcac
     6121 cgacctggcc gacctcgccc accgggccca ggtcagccgc cccatcatgg agcggctggt
     6181 gcttgccggt ggtctggact cggtctacgg catctcgacc gccacgggcg ccggggccga
     6241 tcgatctttc ggcgcgggcg ggctcggcgt ccgagagcgg gtgacccgtc gcgacctgct
     6301 cctgcacgtc gccgagctcg accggtgggt gcggtcgggc ggcagcggtc agggcaaggc
     6361 gctccggggt ggccgctccc ggttcacccg tcgcccggcg acgatgtcgg cgaccggccc
     6421 gggcggcgaa gcggccggtg ctccggtgac cgacacccga gccctggccg ccgcccaccc
     6481 ccggcgcgag gtagccgtcc tcgtccagca gcccggggtg cctgccggcc ccggcaccat
     6541 ccgggccttc gacgtcgccg cgcttgcggc tgcccagtcc caggcggcgg cacccgtgca
     6601 cgtgagcgac cggcataccc agatggtcct gcccctcggc gacacgccca ccctcacccg
     6661 cggcagcggt ctgcccgagc tctccggccc cgagcgggtg cgcgccgagt tggagatcct
     6721 cgggctggac gccagcgccc acgtcgtcgc ccactacgag ccgatgctcg aggcactcgg
     6781 ggtgacccgc tcccgcgacc tgctgagcgc ccgcaaccgc agcgaggtgt gggtcgccgg
     6841 ggtcaaggtc gccacccaga ccccgccgat ccgctccggt cgccgggtcg tcttcctcac
     6901 cctcgacgac ggcaccggcc cgtccgactc gacgttcttc gaggacgtcc agggcccgta
     6961 tgccgccacg gtgttccact cgtggctgct cctcgtgcgc ggagtcgtcc gacgcaccgg
     7021 cgcccgaggc atctcgctgc gcgccaccgg agcctgggaa ctgtccgggc tctgggaggc
     7081 gttcgccacc ggtgggcgcc cacggctgct cgacgcactc gccgctgccg acgccgccgc
     7141 ccatgaccgc gcggctgccg ctgcggccgc ctacgccctc ccgtatgccg tcccccgcgg
     7201 caccgggtgg gccgagccgg ccgccgatgc tgccgcagcc gctgcggacg gcagcccggt
     7261 cggggatcgg gacgacacgg catacgccga tcgggagcgg gctgcacgcc tggaccacgc
     7321 cgcgtccctt gtcggcggtc gaccggccag ccgggccggt ggcatgggcg gggggtcacc
     7381 cacgagcccg gtcggggcgc ctggggcacc gcgccaggtc ctcgtgcacg cctcgggctt
     7441 caaacaatcg ccgtatgccg acacccgacc accgggtgag gacgtcaagt ccgcccgaca
     7501 cctggctggc gctgtcgacc atgccggcct cactgaccag gtcacccccg acgccttcga
     7561 cgccctcgac gcggcgggtc aggggagcgg ggccgggcga ccgccgcgca aactctggca
     7621 ctccagccct ggcagcagtg ggcactaggg tggatgcgtg tccgaggagc agcagcgtcg
     7681 agcccgccgc gccgccaccg gcgtgcgcac cgcggctctg tgggccgccg tcaaagacct
     7741 cgtcaccgag cgcgcggccg ccctcggccg tccgttgcgg gtgctcgacc tcggcggcgg
     7801 caccggcgaa ttggccgtcc cgcttgccga gttgggcccc gacctggtcg ccgacgtcac
     7861 cgtcgtcgac cccagccccg acgcgctcgc ggccctgggc cggcgggccg ccgagtccgg
     7921 cgtccaggcc cgggtccacg gcagccaggg agacgccgac accctcgccg acctgctgcc
     7981 cgagcgggcc ttcgacttgg tctgctgcca tggcgttctt gaggtcgtcg atgaccccac
     8041 cgccaccttg cgagtccttg ccgacgccct cgccgacgac ggccacctct cgttgctcgt
     8101 cgccggccgg ctggccaccg tctgggcccg ggccctggcc ggggagttcg gccaggccca
     8161 caccgctctc accagccccg acggccgctg gggtgctcac gacccgttgc cgcgccgctt
     8221 cgacctggcc cagctgcgcg ccctcgtcga atcagccggg ctgcaggtcg agcactggca
     8281 cggcgtgcgc ctgctcgccg acctggtgcc gtcgacatcc atcgacaccg acgccgagcg
     8341 ggccgccctc gtcgccctcg aggaggcgct cgccgggcac ccggcatacc ccttcctcgg
     8401 tgagctcggt gcggccctgc acctgctcgc ccgccggggc tgatcccggc ctgggagccg
     8461 gccgatgagc cgccgccagt tccgcccgcc gccgcgcacc ggcgacggcc ctcccgacga
     8521 caccgggtgc actgtcctgc acgtcgacat ggacgcgttc tatgcctccg cgtcgctcat
     8581 cgaccgaccc gagttgcgcg gcaccccggt catcatcggc ggcggcggcc gctcggtcgt
     8641 cctctcagcc acctacgaag cgcgcgcctt cggggtcacc tccgcgatgc cgatgacccg
     8701 cgcgcggcgc ttgtgccccc aggccaccgt catcgccccc aaccatgacc ggtatgccgc
     8761 gatctccgac gccgtcatgg ccaccttcgc gtcggtgacc gaccgctacg agccgctctc
     8821 gttggacgag gcgttcctcg acatcgctgg tgcggtccgg cgccttggcc cgccgacgag
     8881 catcgcccag tggctgcgcg acaccattgc cgacgaacag agcatcacct gctcggtcgg
     8941 cgtggcctcg acgaagttcg tcgccaagct cgcctccggc ctggccaaac ccgacgggct
     9001 gctcgttgtc ccgcaggacg aggtgctggc cttcctgcac cagttgccgg tcggtgcgct
     9061 ctggggggtg ggggaccgca ccgaggaagc cctgcaacgg ctggggctgc acaccgtcgc
     9121 cgacctcgcc cacacgccgg tggacaccct gcgccgcgcc ctgggtgatg ccaccggcac
     9181 ccatctgcac gcgctggcct ggggccgcga cgagcgccgg gtcctccctc agcagcggga
     9241 gaagagcgtc ggctccgacg agaccttcgc ccaggacatc gacgatccgg tgctcatcca
     9301 ccgcgagttg ctgcgcttgg ccgatcggac cgcagcccgg gtgcgttcag ccggcctgat
     9361 gggacgcacc gtcaccctca aggtgcggtt cgccgacttc acgacgatca cccgcagccg
     9421 caccctgcgc gaccccaccg acgtgtcccg agagatctat gccgccgcga cggcgctgtt
     9481 cgacgccctc ggcctgcagc gcgcccggat ccgcctggtc ggcgtgcgaa tggaggggtt
     9541 ggtcgagagc gagcgggccg ccgtccaacc ccggctggac gacccggact tcggctggcg
     9601 cgacgccgac cgcgccgtcg accgggccag tgcccggttc ggggcaggcg cggtgcggcc
     9661 cgcggcgctc gtccgggggg agtcggtcgc ccacccgggg agtatgccgc gagggggcga
     9721 atagcgctcg aagcgtcggc ccgggccaac cggcaggtgc tccggctacc acaaccccgg
     9781 atccggacct atcctgatgt cctgacccgg aaccacgggt cggatggcat cgttgtccgg
     9841 atgatggccg ggtttggcct cggaaacgct ggaagccctg ggaggggtgg gtcatggcac
     9901 tgtcggagcg ggagcagaag cttctcgaac agatggagca ggcgctctac gccgaggacc
     9961 ctcgctttgc cacgcgcatt cgcagccatg gcgaccgggg caccaagcga cgcatgctcg
    10021 tcggcatcct cgccgtcctc gccggcctcg ggctggtgtt cctcggcgtc ctgggtcagg
    10081 cgatctggct cggtgcggtc ggtttcgcgg tcatggtcgc cggtggcgtc tgggcgttcg
    10141 cgccagcccg caagaccggc ccggtcggtc tcgtccaggg cgacggctcc acggctgctc
    10201 ggaccgccaa ggcccgcaag tcggggcagg gcttcatgaa catgctcgaa gagcgctggg
    10261 agcgtcgcga ccgcgaccag tggtgaacgc cagctccgag cagcggcccg acaggtgaac
    10321 gctggctgcg cgatcgctca gccggggccg aggacgatga gcccgatgat ccacgtcagc
    10381 aggatggcca ggccggccag caactcgatg cccatcgagt gcaacacggc gaccagcgcc
    10441 gccttggtcg acgtccacgc cgcagagcga tcctgacgac gggtcaactc aacgaggaag
    10501 atccccagga cgaacccgac gagggcgccg acgacgggga tgacgaagaa gccgacgatc
    10561 cccaggagca ccgcgagcag catcgtggag gtgcccacgc cctcacggcg catcttgcgc
    10621 ccaggcagga ggtattgcgt gacgacgccc gcggcataca ggacgactgc gacccagaag
    10681 accacccagg cgccgggcgc ggggtgggca tacgcccaca acaaggtcgc cgcgacggta
    10741 agcagcaacc ccgggacgac cgggacgaca atgccgacca acccgacaac catcagcagc
    10801 cccgtgacga tcgtgaggct gaccgggtcg gcgaagctca ccgcaggccg gtgctctcgt
    10861 ccgcggtctc ggtcaacagc tcagtctcgt cgtggatctc aacggcctga tcggccaact
    10921 ggggaaggtg ctggcgtccg tggtgggcgc agaaatacag ctccccgccg gaatgtaggc
    10981 gggcgcgaat gtaggcccgt gcgccgcagc ggtcgcagcg atctgcggcg ctcaagcggt
    11041 ggaccaaggt ggcgttcacg tctgccttct catctctctc gtgcgggccg acaggtgccg
    11101 acccggtgtt gctgattcga cgtgcaggga cagcaacgct tgctggttgc tgaatccccc
    11161 cacgtcagtc ccaacagtca agcacgccgg ttcgttcccg cgcggtattg atacccatct
    11221 gagacacgcc cgcgcgcccc ggacccggct gtcggacgcc tcgactaccc tgcgtgtggc
    11281 acgccgtcgc cgaacatcaa cgcaccgcag agaggacatc cagtggccac ccgcagcacg
    11341 accggccgca ccgccgcgag tggctccggc gcgggcggag gggactacac cgcccgccac
    11401 cttcaggtgc tcgaaggcct ggaagcggtc cgcaaacgcc cagggatgta tatcggttcc
    11461 accgaccagc gcgggttgat gcactgcctc tgggagatca tcgacaacgc agtcgacgaa
    11521 gcgctggccg gcgtcggcga ccgtatcgag atcatcctcg gtggcgacgg gtcgatcgag
    11581 gtccgcgaca acggccgcgg cattcccgtg gacaccgagc cccggtccgg gctctcgggt
    11641 gtcgagttgg tcttcaccaa gctgcacgcg gggggcaagt tcggcggcgg ttcgtatgcc
    11701 gcctccggtg gcctgcacgg cgtgggcgca tccgtcgtca acgccctgtc ctcgcggctg
    11761 gacgtcgagg tcgaccgggg tggcaagacc tatgcgatga gcttccgccg cggtgagccc
    11821 ggcgtattcg ccgacccgcc gggtccgcac agcccggagg cgccgttcac ggcatacgag
    11881 acggggtcgg agctgcgcgt cgtcgggaag gcgccccggg gcgccaccgg cagccggatc
    11941 cgcttctggc cggacctgca gatcttcctc aaggaggccc gcctcgacga cgacctgttg
    12001 gccgctcgcg cccgacagac ctcgttcctc gtgcccgggc tggccttggt cgtgcgcgac
    12061 gagcggggag caccggacac tcctggcgcc cagggcaccc acgagcaggt cttccaacac
    12121 ctcggcggca tctcggagtt cgccgagttc ctcgctcccg acccgccgct cactgcggtc
    12181 tggcgcctcc agggcaccgg gaacttcacc gaaaccgtgc cggtgctcga cgagcgcggc
    12241 catatgagcc cgaccgaggt cgagcgggag tgctccgtcg acatcgcgat gcggtggggc
    12301 accggctacg actcgcgggt cagctccttc gtcaacatca tcgccacgcc caagggcggc
    12361 acccatgtgg cgggtttcga gcagtccctg ctcaaggtgt tccgccgtca actggagctc
    12421 aacgcccgcc ggctcaaggt cggcaccgac aaggtcgaga aggacgacat cctcgccggg
    12481 ttgaccgcag tcgtcaccgt ccggctggcc gagccgcagt tcgagggtca gaccaaggag
    12541 gtcctcggca ccgcggcggt ccgagccgtg gtcgccaagg tgcttgagca ggagctcaac
    12601 gcccgcctca cctcgaccaa gcgcgacgac aaggctcatg cggcgctgtt gttggagaag
    12661 gtcgtctccg agatgaaggc gcggatcagt gcgcgccagc acaaggagac ccagcgtcgc
    12721 aagaccgcct tggagtcttc caccctgccg gccaaactcg ctgactgccg cacccacgac
    12781 gtgctgcggt ccgagttgtt catcgtcgag ggcgacagcg ccctcggcac cgccaagttg
    12841 gcgcgttcct cggattacca agcgctgctg ccgatccgag gcaagatcct caacgtccag
    12901 aaggcctcgg tcgccgacgt cctgaagaac accgagtgcg cggcgatcat ccaggtcatc
    12961 ggggccgggt cggggcggtc cttcgatctc gaggcggccc gctacggcaa ggtcatcatc
    13021 atgagcgacg ccgatgtcga cggtgctcac atccgcaccc tgctgctcac cttgttcttc
    13081 aaatacatgc gcccgctcgt ggccgccgga cgggtgtatg ccgcgatgcc accgctgcat
    13141 cgcctcgaag tcatcggcgg cccagggcgc aagaacgaat acgtctacac ctacaacgag
    13201 gcgcagatgc gctcgacgcg ggcggcgctg gaacgcaagg ggcgcaaggt caaggagccg
    13261 ccccagcgct acaaggggct cggtgagatg gacgccgacc agttggccga cacaaccatg
    13321 gacccgcgct tccggacgct gcgtcgggtc accctcggcg aggccgagga ggccgcccgg
    13381 atcgccgaag gtgtcttcga cctgctcatg ggcaacgacg tggcgccgcg caaggacttc
    13441 atcgtcgagt cggccaagac cctcgaccgc gaacgcatcg atgcctgacc tacctgggat
    13501 cacgcggcca cgcaccgacc ggcgagcgcc gggacggaac gtcggcgccg catcagacgt
    13561 ctaccatggg ccggacccga cctgagtcgg cgggtgagca gcatcgacag ggggagtgag
    13621 catgagcacg acgatcgacc cgggaccacc gccgggagcg caggcggcat accccaccag
    13681 tgcgtcgacc accccgcgac cgggcgtcac ggcgatcccg ccggaggatc tcgcctgggc
    13741 gcgctcgacc gtcgccacca tctcacgctc gttccaggag cgcgtcgtcg gccaggtgcg
    13801 actgcacgag accctgctgc tcgggctgct caccggcggc cacatcctgc tcgaatcggt
    13861 gcccggcctg gccaagacga ccgcggcagc caccctcgcg cgcaccgtcg gcggctcgtt
    13921 caaacgaatc cagtgcactc ccgacctgct ccccagcgac atcgtgggca cccaggtcta
    13981 cgacgtgcgc accacccagt tcgagacccg gctgggtccg gtccacgcca acctcgtcct
    14041 gcttgacgag atcaaccgca gctccgccaa gacccagtcg gcgatgctcg aagccatgca
    14101 ggagcggcag acctcgatcg ggggtcaggt ctacccgctg cctgagccgt tcctcgtcct
    14161 ggccacccag aaccccatcg agcaggaagg cacctacgtc ctgcccgagg cgcaactgga
    14221 ccgcttcatg ctcaaggacg tcctggatta cccgaccctc gacgaagagg ccgaggtggt
    14281 ccggcggatc gacgccggca tctatctcgc cgagcagacc cccgcggtca gcctcgccga
    14341 cgtgcgccga ctgcaggcgt tgacccgtgc ggtctacctc gatgacaccg tcatccggta
    14401 tgccgtgggg ctggcttacg tcacccggca cgccgagcgg tacctcgacc cggcgctggc
    14461 ccggtacgtc gagttcggtg cgagcccccg ggcgtcgatc gccttcgccc aggccgcccg
    14521 tgcgcgggcc ctgttggccg gccgcgacca tgccgtgccc gacgacgtcc aggccctggc
    14581 ccatcgagtg ctccgccacc ggatcatcct tggcttcgag gccgtcgccg acgatgtccc
    14641 ggtcgagcgg gtcatcgacg ccatcatcac cgcggtccgc accccctgac cgggccgacc
    14701 ggcatacctg cgctagccgc cccgaccaga cgagagcacc cgtggccgcc ctgctcaccc
    14761 gagtgaagag caaactcttc atagtcgcgc accggcgcac gtggggcttg ctcgacgggg
    14821 agtatgcctc ggtcttccga gggcgcagcc tcgactacga cgacctgcgc gaatacatcc
    14881 ccggcgacga gatccgcgac atcgactgga aggcgaccgc tcggcacggg gccccgctgg
    14941 tcaaacgcta cgtcgccacc cgtaagcaga ccgtcctgct gctggtcgac accggtcgct
    15001 cgatggcggc catggcgccg tcgggcgagc cgaagaagga catcgcgatc cagatcgccg
    15061 gcatgctcgg ctatctcgcc gtccgccacg gcgacctggt cggcctcatc agcggcgacg
    15121 gtgagagcac caggatgctc gagccggctg cgggggaggc caacctcgag cgtctgctgc
    15181 gggtcatcga tcgcgcgagc acgcttgagg ctccgcccag ccggttctct gcgcaactgc
    15241 actacctcgc cgagcacgtc cggcgacgga tgctcatcgt ggcagtgacc gacgatggcg
    15301 gcctgggctc tgacgacgac ccgttgttgc ggcgactgca cgctcaacac gaggtgctct
    15361 gggtggttgt ggccgacgcc gacccgacgc gtttcgaccc gcgcgccgat gcctccgagg
    15421 tcaccgacgg cagcgctctc ccaggggcgg tgcggggcgg cgaacgtctc caggcggcat
    15481 accagcaggc tctcgcgggc cgacgcgagg cgaccgatca gcggctgcgg cggctcggga
    15541 tcgcgcgcgc tcgcgtgggt ggcacgtcgg aggtcgtggg cagcatgttc gcgctcctgg
    15601 agaggcagcg ccgccgtgca cgttgacgac ccgttctacc cgccgatgac ctaccactgg
    15661 gtctggacgc tgctcgcctg ggtggcgatc ttggccgtgc ttgcctgggc cgggttcatc
    15721 tggtggcgca cccgtcgcgt ccggcccaca accccggccg ggcccgttcc catccacccg
    15781 gggtggcggc ttgcccgcgc gaagcacgac acgatgaccc ggatcgacca gatcgtctgg
    15841 gaatgcgagc agggcagggt cgacgcccgg acgtcccacc gtgagctcag tgccgcggtg
    15901 cgggccttca tcgatgacgt gtcgggggcg accacgtcgc ggatgaccct gtctgacctc
    15961 ggctcccgcg gcccggggct ggctccggtg acacacgtcg tcctgcagct ctaccccggc
    16021 gagttcgggc ctgacccggc gcgacccatc cggcctgccg ccgaagcggc caaggcggtg
    16081 gtggcccaat ggcactgaaa caaccgtgga tcctgccgtg ggcggccctg ggggctctcg
    16141 cgattgccgc gctggtgtgg ttcgcgctgc gccgtcgcag gccgccggcg gcgctgccgt
    16201 tggccaacac cgatctgctc accagccttc cggagtatgt ccacgcgatc tggcgacacc
    16261 gactgatgac ggtcctgtat gccgccgcgg cgggtttcgt cgccgtgctc gcgatcatcg
    16321 gcgcggcccg gcccgtggcc acgaccgtca tcaaccctga gaaggccaac cgcgacatcg
    16381 tcctctgtct ggacgtctcg ggctcgatga ctactgccga cgccgaggtg gcggagacgt
    16441 tccgatcgct tgccaaggag ttcaaggggg agcgtctctc gctggtcatc ttcaactcct
    16501 cggccgtgcc ggtgtttccc ctgaccgacg actacgactt cgtcgaggag cagttgtccg
    16561 acgcccagcg ggtcttcgag gggagcttcg accacgagtt ctacgccggg acactcaacg
    16621 ggcaggggag ttcgctcatc ggtgacggct tggccacctg tgtgcgcagc ttcgaccacg
    16681 gcaacctgac ccgggcccgg tcgatcatcc tcgcgaccga caatctcgcc gcggggaggt
    16741 cgatcttcag cctggcgcaa ggcggaacga tggccaaagc tgccggcatc cagatctacg
    16801 gcctcaaccc ggctcatcgc gcggggagtt cgacggcgac cgagatgcgc tcggtcgccg
    16861 agggcagcgg tgggctctac taccccttga acagcgtcga cgcggtgagc cggatcgtgt
    16921 ccgccgtctc tgctcgggag gcctcccgga tccccggagc accgatcacg ctcgtccacg
    16981 accaaccggc gtcgtgggtc gggtggacct ggctcggggt gcttggcctg ctcggggccc
    17041 tgcgacggtg gcgccgatga ctcttcttcc ggtcatgtct gtcggcgtcc tggtgctggc
    17101 ggtggtggtg gcgttggcgc tggtgtggtg gccgcagccc ggcccccagc cgagtctgcg
    17161 aacgcgagtg cgccgcaccc tgctcgtggc cgcggtgctc gtggcctcgc tgcgcccggg
    17221 cctgcccggt gccgaggtgc gggccagcgc gtccgagctc aacgtgttct tcgtcgtcga
    17281 cacgaccacg tcgtcgatgg ccgaggactt cggcaccgac ccgcggatgt cggggatgcg
    17341 cgcggacatc acggccatct cagggcgaat ggcgggggcg cggttcgcgc tcatcacctt
    17401 cgaccaggcc gcagtccttc gtatgccgct cacctccgac ggcgcggccg tcgccgccgc
    17461 ggctgagacg atgctgccgg aaacgtccgc ctggtcacgg gggagcagcg tgaccgcggc
    17521 cggaccgttg ctgcagcaga ccctggcccg ggtcgccgcc gcgcatccgg agcgggcgcg
    17581 ggcggtcttc tacctcggcg acggtgaaca gacgtccggc agcccaccgg cagcgctggg
    17641 agtggaccag agcctggtca acggcggtgc ggtcctgggt tatggcacga ccggcggagg
    17701 ccagatgcac gagaccggag tgcccgggtc gcgtgcggat gccggctggt tgctcgaccc
    17761 gagcaccggc aagccggcgg tctcggtcat cgacgaaggc caattgggct cgatcgccca
    17821 acaattgggg gtcccctacc tgcatcgctc gccgggtgag agtgtcgacg ccgtcctggg
    17881 gctggtggat ctcaccggcc tcgatcgggt gaccgtggca caggacgggc cgctgtcggc
    17941 gggccgtgag gagttctact ggatcgccct gctggcggtt gccgggcttg tggcctggga
    18001 ggtcgggctg gctctggccc ggttgtgggg gctgcgccgc ccgaggcgag cacgcggcat
    18061 accggaggat ggcggcatac cggcagatcg ggacggccac gaggagaagg tgcggtcatg
    18121 acgctcgctc cccacccggc gccggcgcgc cccgcagccc tcgatccgga cgaaccggcg
    18181 gccgcccgac acgcgcggct cgccatgcgg cggcgcctga tcagttggtc gatcgggccc
    18241 gcgttcgtgg ccatccttgt ggccgtcaaa ctgctgacga tgagcgccgc cgccacacag
    18301 gcagttgacg gcttcttcgc cgcggcagcg cacacggtct tcgaggcggc tgatcggatg
    18361 tcgttcctca acgttgtcga gcggcacaag gctcccttcg cgcgcggcga cgccttcgtg
    18421 ctggccggcg atctggacag cgcgcgggcg cagttcgagc aggcgttgga gttggcgccg
    18481 cccgactcga tcgactcgtg tcagatccgg gtcaatctgg cgctggcgct ggagaaactg
    18541 ggcgatgccg ccaaggcggc cggcgacgag gtcgcggcga aggactactg gacccgcacc
    18601 aaggcggtcg ccgatgcggc gcctccgggg tgtttcccgc cagcggcgga cggggccggg
    18661 cagaaggccg aagcggccgg tgaccgggcc gaacagaagc tcaaccccca gcagtcctcc
    18721 ggcgttgacc agtcgagcga tgcgcagcag caggccgagc agcagcgcaa ggcggatgaa
    18781 ctcgaccagc agacccatga gaaccagcag gaccgccagc agaacgggcc ggacgggcaa
    18841 ggaaacccgg cgggtggggg cggttcggct aacccggcgg ccaaaccctg gtgagtatgc
    18901 cgctcgcgcg gctccgggcg ggtgtccccc cagcggtggc taggttgagc tagggcttcg
    18961 caggggctgt ggtcgacccg cggatgacga gttcgaccgg gaaggtctgg tcagggatcg
    19021 ctgaggggtc atagagcccg agaagggcga gcaaggtgct ggccgctgcg actccctggg
    19081 cggcgacatc ctgacgcacg gtggtgaggc cgaatacggg ggccaactcg tggtcgtcga
    19141 tcccgacgat ggacaggtcc ctcggcacct ccagaccgag ttggcgggct gcgccccaca
    19201 caccgagggc catctcgtcg gaggcggcga ggaccgcggt cggccggtcg ggaccagaca
    19261 gcaaggccat ggccgaggcg cgcgagtctc cagcggtcca gtcggagtcg agcacccaag
    19321 tcggcggggt gttgatgccg gctcgggaca tcgcctggtg gaaaccctcc agccggtcga
    19381 tcggtgtctt tcgcggtgcc gcctcggggt agtccctgcc gacataggcg atcttgcgat
    19441 gacccagcgc caacaggtgt tcggcggcca gggcggccga gccgatgtcg tcgatgccca
    19501 cgcacgggga gtcctcgaac gggctgccga cggcgaccac aggcagcccg aggcgacgga
    19561 cgaggtcacg ctcggcgtcc tgcagctcga cgttgatgac gatgaggccg tcgacccgct
    19621 tgaagagcat cccctgggtg agggagaggc gggacatgga ggtctcgctg cgctccaggt
    19681 ccatgagcaa gatgtggtgc tggtgctccc gaagggtctt ctcgatggca ctcatgatgc
    19741 cggtgaagaa ccaccgtgcc atgaagggcg tgatgatccc gatcaggcgg ctgcgtcctg
    19801 aggccagacc ggaggccgtg gggctggcga tgtagtcgag ctgagcagcg gcttggacga
    19861 cgcgttcgcg ggtctctggg tgcaccttgt ccagtccgcg tagggcgcgc gacacggtcg
    19921 caacggacac gccagccgcg cgagcaacat cagcgattgt cggttgttgc acgccgacct
    19981 ccgtctgatg acgcccgcag acgggcagaa tggttgactc ccctgcaaca acccaccgca
    20041 acaggctgcc cggatttgac acaaagcgcg cgagtgcgcg ctctactcgt caatctactc
    20101 tcaacccaca ggttgcaaca aagccggacc ttcgagcgcg ttggcctgac ccggatggca
    20161 ggtgaccagc gatccggtgg ggagctcatc agaccggcac agcggcggta ggcagcgcac
    20221 gtgcacgcag cagcaccagt tggtcgggat tgagcagggg cgcctggata cccacctcac
    20281 gcaggacccg cccgggcagg gtgacgcccg agggacccca cccgggtggg aaagtggcgg
    20341 ctgccgcatc accgggtgcc tgggcactga cgtggtagtc ccgcgccggg tcgagcccag
    20401 gcagacggac ccggccggcc ggccaggtga cggtgaggtc gagggcggcc aggcggtaga
    20461 gagcgtcgga ccggtccgtg gcgacgactc cttcgagcag tagcgccggg ttgggcaggt
    20521 cggcgtgcac gacatcgccg gtgtgcagca actcacggac ctccttgtgg aaggcgatcc
    20581 aggcagccag ggtgtcgagg tcggctgacg aggccgtggt gaggtcccac tcgaccccga
    20641 ggtgccccca catcgcggtg cccgctcgga agcccaggtg cagcagccgg tgcgtcgagt
    20701 ggtcggctcc cgacccgacg tgtgtgccga gaagttccgg tggcagcagc agcccggtcc
    20761 agcgcaccag acgttgacgc tcgtgggcgt cgatacagtc cgacacccag acccggtcgg
    20821 tgtgttcgat gatgcccagg tccacccgac caccgccgcc gcagcacgac tcgatctcca
    20881 gccccgggtg gcgagccttg agctcggcca tgaggcgata gaccgcctcg gtgtggatgt
    20941 ggacgcccgg gctgccgtca ggggagtgcc cggcgtcgac gagcggccga ttgtggtccc
    21001 acttgatgaa ggcgatccgg tattcgccga ctagcgtgct catccgctcg aggacgaagg
    21061 cgtatgccgc cgggtgaccc aggtcgagca cgtgctggta gcgggacggc aggcccggcc
    21121 cgtgatcggt ctggaagacc cagtcgggat gctcgcgggc gagatcggag tcgaggttga
    21181 tcatctcggg ctcgaaccac agcccgaagt ccatgccgag cccgtgcaca tggtcgacca
    21241 acgggtgcag cccatcgggc cagactccct cgtcgacgaa ccagtcaccc aagccggcct
    21301 gatcggagcg ccgatgccgg aaccacccgt cgtccaggac gaagcgctcg atgccgagct
    21361 cggcggcccg gtcggccagg gtgatgagct tgcccaggtc gtggtcgaag tagacggcct
    21421 cccacgtgtt gagcaggacg gggcgcgggc cacgcgggtg gtggggccgg gcccgcagga
    21481 agcgatgaac gcgctgggag aaggcgtcca acccctcgcc ccaggaggcc accagccagg
    21541 gggaggagta gctctcctgc gggtgtagga cgccctcgcc cggaaggagg acctccccgc
    21601 cccgcagcag ccgccagccc gccgtcgagt gctcggccag gacgacctgg ttaccgctgt
    21661 gcgccacatg gacgccccag acccgtccgg accggaagcc gaagccgggc tcaccgtcac
    21721 acatgaccgt cgccgagtcg tggcccggcc gtccgcccca cgcctcccgc acccaggccc
    21781 cggggtcgaa gggccgccgt tgcggggtcc gctcatgggt atgccgtccg gacatgtcga
    21841 gcagctcacc cgcctccgcc ggcaccggca aagcgggcaa caggtgggcg acctcgtagt
    21901 cgtcggcgcc gaggttgcgc acctgggcac ggacccggac caatccgctg gggtgcacct
    21961 caatctcggt cgtgacttcg agggcgctgg gccggtccag gccgacgctg cgcaaccgca
    22021 ccgccccggc ggcgccgtcc gcccagtccg ccgacccggc ggtctcgacg cggtgttcga
    22081 cccgatcgaa ggacaccgac cacgagcgtc cgccggctcg gctgccgagc agcccaggtc
    22141 ggccgagcca ccccgacgag tgttgcggca ggagcgcgac cgctgcttgc gagctgatcg
    22201 gcgagtcgac gtagggaggt gtcaacgcga tctgcagttc atccagcgcc tgggtcgaca
    22261 cgtcgccgag gtcgggtccc cagtgcagca cgcacggcag ggcggcgtcg tccagacgcg
    22321 cgaccacgct cgtcccaccg aggcgcaagt ggagcatggt cggcacggac ggggcggtag
    22381 tcatggcgtg cctccggtga gatgttcccg ctcgatatcg gcgcggggtt gacggcgctg
    22441 gactgggcac ccggctgccc ctcttctgcg ggctggtcgt ttgcggacgg gtctcatgct
    22501 cgccagctcg gcgcgtagac gatcgtcggg acggcggcct taccgcatga ctcgggcagc
    22561 atgggtccct gccgggccac gacgtccacc tgctcgcgca gatcatccat cggatcgtcg
    22621 aggtcgcggc gatcggtctg ccggcgcagc caggcatcgg cggcctgctc cgcagggggg
    22681 aggtgcatcg gccctcgatc gtgcacccgc ccgagcagcg ccggtgccag cctgccggaa
    22741 gcggcggcgt cgagggcacc ggctgccaac tcgaggtcga gtcgggccag cacgcgaccc
    22801 cacggcagca gcactgcggt cggtgcgaag cggtgccctc cggtatggct cgtctcccag
    22861 acctggtccg gggcggccgt ggcgaacccg ccggagacga acgcgctggt gacgacccgg
    22921 ccccgctgag cgcagcacgc gtcgcgtcgc ccgttggtgc agacgagcag gagtggcacg
    22981 gtgtcgaggg ctccacccag gcggtctgcc gtcgccccag cgctggccag ttcgtcggcg
    23041 ccgaggccca gcagcatcgc cggatcggtc aggtcggctc gcacgagcca tccgcccgga
    23101 cccgtgcgcg cgatgtacgt gcgccgggtc gactgctgcg gtctggcccc atcggcaccc
    23161 gcgcccgcac cggcgcccct ggcctccggt tcggcgtcgg tctgaggggc ctgttcggca
    23221 tggcggccgg gccgacggat gagcagcaga cgtccgccca acccggacac gtgggcatcc
    23281 agggccgccc ccaaggccgg gtccaggtgc gattgggtgg ctgcgacgcg gccccacgga
    23341 ccgttctgct ccagagccac gacgaagtct gctcgcgcag ccgtgcccca cgcggggagc
    23401 gcaccggtat cccacacccg cgagcacgca gggtcgacga tggtcactcc ggcatccttg
    23461 cgtaggtgga cgatgcgagt cgcattgctc tcacctggac ctgacgacaa tcatctgcca
    23521 ggtcgccagt ggcggcaggt gcacggtggc gtgggtgccg tccaccgcga ccaccagcgg
    23581 ctccaggtga ccactcgcgt ccgggtcggc ggcatacacc gccggaacgt cgggcccgac
    23641 gcgccgcatc cgcagagtgc ccgccccggg agttcccggc ggtccctgcg gtgcgtccca
    23701 gcggcagtcg gtggccccag agaggttgat gaggtgcacc accagggcat caccgaccgc
    23761 ggtgatccgg cgccacacga ccccaggcct ggccgtaccg ctcactgtcg cggcggcata
    23821 cgagacgtcg aggtcatcgt tgtaggaggc cgcgtaggag ccggtgacct cgacgatccc
    23881 cgatgggatg agcaggtcgt ggtgctcggt gaggaagtcg tagtagcgcg ccaacaggtc
    23941 gacggtcccc ggctccgcca cgtggttgcg gacgtagtag gggtccacga ggatccggtc
    24001 ggcctccccg gccagcaggt gggtcgcgcc gtgggagaac accgtggcca tcgtcagggc
    24061 ggtcgcgcgg tcggcttcct cgcggggggc gacggcatac acctgctggt atgccgcgat
    24121 gacgaccggc cgcccgccgg ccacggaccg cgcgcggccg atcacctgcg caagggctgc
    24181 gtgggtggag tggggttccc acacctcgat gtagacggcg tcctgcggtg cctgagcact
    24241 ggcccaggtg gggaagtcgt tgacgttgtt gaagaccagc cgggcgccag gcacgcgctc
    24301 gcggaccgat tcgatgaggt gcacgaagct ctcggtgagg tcgacccggg tgccgtccgt
    24361 gcgcagtgcc acc
//
LOCUS       DAOMZY010000012        23168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_1884_length_23168_cov_6.262104, whole genome shotgun sequence.
ACCESSION   DAOMZY010000012 DAOMZY010000000
VERSION     DAOMZY010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 23168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23168
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1884_length_23168_cov_6.262104"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..1551)
                     /gene="glmM"
                     /locus_tag="PLK64_01535"
     CDS             complement(196..1551)
                     /gene="glmM"
                     /locus_tag="PLK64_01535"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPV78451.1"
                     /translation="MGRLFGTDGVRGLANQDITAELALDLAVAAAHVLGDAGEFAGHR
                     PFAVVGTDGRASGEFLSAAVMAGLASAGVDVMDAGLLPTPAIAFLTADLGADLGVMLS
                     ASHNPMPDNGIKFFARGGQKLDDVVEDAIEARMQEPWTRPTGAAVGRIRRLEDGATRY
                     VRHLLTTLPHRLDGLHVVIDAAHGAASMVSPWAFREAGAFVTVIGAEPNGENINAGYG
                     STHLARLQRAVIENGAQLGIAHDGDADRCLAVDARGEVVDGDQIMCILAMAMRERGTL
                     AHDTLVATVMSNLGMLQAMTREGITVRQTGVGDRYVLEDMKAGGYSLGGEQSGHVIFL
                     DHGTTGDGVLTGLMLASRVAQTGHPLAELAAVMTRLPQVLVNVKNVDKDAVDGHPTVQ
                     SAVAEAQRELDGSGRVLLRKSGTEPLVRVMVEAADHHQAQAIADRLADVVKSQLSLSD
                     A"
     gene            complement(1714..2217)
                     /gene="rpsI"
                     /locus_tag="PLK64_01540"
     CDS             complement(1714..2217)
                     /gene="rpsI"
                     /locus_tag="PLK64_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPV78452.1"
                     /translation="MADNTTDVALDVDEPELSEYTTETQNAPVGAAARKPSASAPGAA
                     TGRRKQAIARVRIVPGTGEWTINGRTLDSYFPNKVHQQIVNEPLKVLELDGAYDVLVR
                     VHGGGPSGQAGAVRLGVARSLNGVDEELNRPTLKKAGFLTRDARVPERKKAGLKKARK
                     APQYSKR"
     gene            complement(2217..2660)
                     /gene="rplM"
                     /locus_tag="PLK64_01545"
     CDS             complement(2217..2660)
                     /gene="rplM"
                     /locus_tag="PLK64_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPV78453.1"
                     /translation="MRTYTPKPGEVTRAWLVIDATDVVLGRLASQTAVLLRGKHKPTF
                     APHVDTGDFVIIINAEKVALTGNKAQLKRAYRHSGFPGGLSSMSYAELLDKDPRRAIE
                     KAVRGMLPKNSLARQQLTKLKVYTGDVHPHAAQKPVPFEITQVAQ"
     gene            2920..3549
                     /locus_tag="PLK64_01550"
     CDS             2920..3549
                     /locus_tag="PLK64_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV78454.1"
                     /translation="MLGFAPLTPDQIRVLAHPLRSRLVDRLRRRGPATATTLAKELDT
                     NSGATSYHLRRLAAYGLVQPAGGGRGRERRWEASARRHTLPGGADAGVGGSGGGASGP
                     IASTRGADPDTESSLDWLDRDYVRHFAEKAELWLDAAPEWPAGWTDPCGLRDGMVLVT
                     APQLADLRAELDGVLARYRRVGQGNPKAKRVAVYTSCQPLDLDSPPLTP"
     gene            complement(3605..4207)
                     /locus_tag="PLK64_01555"
     CDS             complement(3605..4207)
                     /locus_tag="PLK64_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HPV78455.1"
                     /translation="MNIEILLFDGFDEFDAMAPWEVFAGLAQVTDRVRASFVTLDGAG
                     PVRGDHGAVVMAHGALSERPDLLWVPGGGWLDRSPTGAWGLVQAGEVPRAIAAAHAAG
                     TVVASVCTGALILAAAGVLAGHRATTNPHALDDLRAFAGVTVLEARVVDDGDVVTGGA
                     PACALDVALHLVGRFVGPDIAAAAARELQYVVPAVDAFVG"
     gene            4280..5233
                     /locus_tag="PLK64_01560"
     CDS             4280..5233
                     /locus_tag="PLK64_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004565683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV78456.1"
                     /translation="MADTAVQTARHRVVGLLVGEVVMFDLAIADQVFGRTDGYTWSVC
                     GVRRGWLPTTTGLAVRVDHGLAALDEADTIVVPGIGDEAWPVPAAALRRLRAAHARGA
                     RVISVCTGAFVLAAAGLLDGRRATTHWRYAGLLASSFPAVEVDPAVLYVDHGTIATSA
                     GLAAGIDLCLHLVARDLGADRAAAVARNMVVPPHRRGGQAQFVEALSATHEGTGLAEV
                     LDFMTVRCAEPLTLADLARRAAMSPRTFTRHFRAQTGVSPKQWLLARRLEAARRLLES
                     SDLSVAEIAVRTGFGTPLALRQQFTRVLRTTPTAYRRTFAG"
     gene            complement(5301..6983)
                     /locus_tag="PLK64_01565"
     CDS             complement(5301..6983)
                     /locus_tag="PLK64_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPV78457.1"
                     /translation="MGHVDVNQVSFDLPDGRPLLREVSLRVGEGARIALVGPNGSGKT
                     TLSRIISGDLAAHSGAVTRSGGLGVMRQFIGSVRDESTVRDLLLSVASDKVRAAAAEI
                     DRTELAVMEHDTERDQLAYAQALVDWGDAGGYEAETLWDTCTVAALGVPYERAQWRPV
                     TTLSGGEQKRVVLEALLRGPEEVLLLDEPDNYLDVPGKRWLEDALIASPKTVLFISHD
                     RELLARVATRVATLEPGAAGSTLWVHPGPFGTYAAAREDRNSRLEELRRRWDEEHIKL
                     KALVVMYRQKAAYNSDMAPKLQAAETRLRRFLEAGPPEAVPVTQRLRMRLTGGRTAKR
                     AIVCERLELTGLMQPFDSEIWFGERLAVLGSNGSGKSHFLRLLACGGSDPDVEHQPVG
                     ETRPEPVRHTGSARLGARVRPGWFAQTHHQPALLGRTLAEILHRGDEHRDGRGREEAA
                     KVLDRYGLAKAAEQTFESLSGGQQARFQILLLELSGATMLLLDEPTDNLDLDSAEALE
                     AALDAFEGTVVAVTHDRWFARGFDRFLVFRADGRVIESVDPVWDEGRVDRAR"
     gene            complement(7053..8420)
                     /locus_tag="PLK64_01570"
     CDS             complement(7053..8420)
                     /locus_tag="PLK64_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV78458.1"
                     /translation="MTSEVLLPPGVERGPATVRAFRFVLLNTLIANVTTSYLWFALTF
                     WAYLETRSVLATSIIGGSFMLLIAVFGTVFGSVVDRHVKKNVMIGSNLVTLVTFVAAG
                     GLFLAFPTERLIDWNGPVFWVFVLVILVGGVVESMRNIALSTTVTLLIPADDRDKANG
                     LVGTVQGLAFMVTSVFSGLSIGLLGMGWTLVIAIGLTALSLGHLLTIPIPEVPPTRHE
                     SDSAGHFDVRSALEAIRAVPGLMALVLFATFNNLVGGVFMALMDPYGLTLFSVESWGI
                     VLGVTSIGFIIGGAWVAKFGLGATPLRSLMLANVVAAVLGMAFTIREVWWLYAGGILL
                     YMCVIPIAEAAEQTILQRVVPYRTQGRVFGFAQSVEAAASPVSAFLIGPIAQFWLIPY
                     MASDAGKERFGWLLGAGEARGIALVFLLAGAIMLVTVLLALASRPYRQLSRAYASAPP
                     SEASA"
     gene            complement(8452..9291)
                     /locus_tag="PLK64_01575"
     CDS             complement(8452..9291)
                     /locus_tag="PLK64_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HPV78459.1"
                     /translation="MSVRVRLDLGYDGTDFAGWAAQPGQRTVEGELSRALTTLLRAPE
                     PVRLTVAGRTDAGVHARGQVVHADVDEAAYLAVVGRSRRSPEEAAVVRLRGLLGSDIL
                     VHRVCRAPAGFDARFAAVWRRYSYRIADSLSTADPVRRRDTVWLPDPVDVDVMNQASA
                     GLLGLKDFAAFCKRRAGATTVRTLLDFSWARGADGVVEARVVADAFCHSMVRSLVGSV
                     VPVGLGREDAAWPGQVQAAAARNGRVKVMPAHGLCLEEVGYPADAALADRARESRARR
                     DGA"
     gene            9386..12106
                     /locus_tag="PLK64_01580"
     CDS             9386..12106
                     /locus_tag="PLK64_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPV78460.1"
                     /translation="MPTWTMAFGDPGLDLPTGGGKAVNLGRMTRSGFPVPDGFVVTTT
                     AYRHAVAATAGLADLIDAALAEGDPTGPATAESASRAIRAAFRAAPVPAEVAEAVRAA
                     YLALDEGPVAVRSSATAEDLPDLSFAGQQDTYLGIVGEAAVLDAIRDCWSSLWTPRAI
                     VYRRRNLVPDAAAALAVVIQRLVPADVSGVLFTANPLTGHRGQMVLDATFGLGEALVS
                     GQVEPDHVVADAASGRVLSRTVGAKAVATVVSPTGGVTTETRQASGESAVTDSQVAAL
                     VALGRRVQAEYAAPQDIEWAIHDGTIHLLQTRAITSLYPVPDGAPPDSVWLSFGAVQG
                     MLAPMTPLGRDAIRCILSGGASVFGHTVDERTNPYLGVAGERLWIRLDRLLRNPLGRR
                     GAPEFLRFVDPSAAAIADTLLGEHWLRPLPRATSRRVAAQTARDLARFGRVAVPRIAR
                     ALARPLPARDRFDDDIDDLIATAREREADAAAQAEPVARLAARARALRADLRGAFPTL
                     LPDAAPLVAPAVVMLRVLSELTDATTDGHGVSPLAMEVTRAIPRNPTTEMDLTLWAVA
                     EAIRADPDATFAFQALTPTELAARYAARRLPDGAQAALDAFLTAYGMRGVGEIDLGRP
                     RWREDPTDLLQTLTVYLDLPEDAAPPAVFRRGEQAAAVAVERLAAVASGMPRGHLRAR
                     TVRWLASRIRALAGGREQPKFAVVQVMGVIREGLLASGADLVALGVLDRPDDVFFLHL
                     SELTGLTQASLGGLRPAVEARRAAWSTEIRRARVPRVLVGDGRAFHEGVARTADLADL
                     PPGTLVGSPVSPGVVEGLVRVVHDPAPSGLQPGEILVCLGTDPAWTPLFLTAGGLITE
                     VGGMMTHGSVVAREYGIPAVVGVDAATTRLVTGQRIRVNGSVGTIELLED"
     gene            complement(12225..12989)
                     /gene="rplQ"
                     /locus_tag="PLK64_01585"
     CDS             complement(12225..12989)
                     /gene="rplQ"
                     /locus_tag="PLK64_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPV78461.1"
                     /translation="MPTPSKGPRLGGGPAHERLLLAGLATALFEHDRITTTEAKAKRL
                     RPLAERMVTFAKRGDLHARRRVLTVVRDKGVVHRLFTEVAPDMAQREGGYTRIIKIGP
                     RKGDNAPMAVIELVREPLSAKKATVKEAEAATKRAAADKKKAAAATEAAVEAPKDLEI
                     DDAVEAAPATEVAGLVDAPEAAATEAAELPAGAAAPLADGSAPEGYTVKGNADSGLYH
                     EPDGRWFDATVAEFWFKTAADAEAAGFKKAGSKSED"
     gene            complement(13067..14098)
                     /locus_tag="PLK64_01590"
     CDS             complement(13067..14098)
                     /locus_tag="PLK64_01590"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPV78462.1"
                     /translation="MLIAQRPVLSEETVADNRSRFVIEPLEPGFGYTLGNSMRRTLLS
                     SIPGAAVTSIRVDGVLHEFQTIPGCKEDVTEIILNIKSLVVSSEHDEPVVMYLRKQGP
                     GAVTAADIAPPAGVEVHNPDLHLATLNAKGRIEMELTVERGRGYVSAQQNKSGDQEIG
                     RIPVDSIYSPVLAVTYKVEATRVEQRTDFDRLIVDVETKSSMTPRDAMASAGKTLVEL
                     FGLARELNVEAEGIDMGPSPTDAALAADLALPIEDLDLTVRSYNCLKREGIHTVGELV
                     GRSEADLLDIRNFGAKSIDEVKAKLAGMGLTLKDSPPGFDPASIADTYADDYDDLDEG
                     RSLAEDEQF"
     gene            complement(14302..14910)
                     /gene="rpsD"
                     /locus_tag="PLK64_01595"
     CDS             complement(14302..14910)
                     /gene="rpsD"
                     /locus_tag="PLK64_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPV78463.1"
                     /translation="MARYTGPITKKSRRLKVDLVGGDKNFEMRPFPPGQHGRRRITEK
                     EYLTQLQEKQKARFTYGVMEKQFRRYYQEAATRPGKTGDNLLQILESRLDNVIYRAGL
                     ARTRRQSRQLVSHGHFEVNGHRVDVPSYRVEQFDIITVRKQSVETFPFELARQTFGDR
                     TPPAWMQVVDGSLTVLIHQLPVREQIDTILTEQLIVELYSKN"
     gene            complement(14943..15353)
                     /gene="rpsK"
                     /locus_tag="PLK64_01600"
     CDS             complement(14943..15353)
                     /gene="rpsK"
                     /locus_tag="PLK64_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008747488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPV78464.1"
                     /translation="MPPKSRTAQGAAKKVRRKEKKNVAHGHAHIKSTFNNTIISITDP
                     TGAVISWASAGQVGFKGSRKSTPFAAQMAAEAAARRAMEHGMRKVDVFVKGPGSGRET
                     AIRSLTATGLEVGAISDVTPSPHNGVRPPKRRRV"
     gene            complement(15399..15776)
                     /gene="rpsM"
                     /locus_tag="PLK64_01605"
     CDS             complement(15399..15776)
                     /gene="rpsM"
                     /locus_tag="PLK64_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPV78465.1"
                     /translation="MARLIGVDLPRDKRVEIGLTYIFGVGRTTASKALVATGISPDTR
                     VKNLTDADLVSLRDWLETNVKMEGDLRREVAADIRRKVEIGSYEGIRHRRGLPVRGQR
                     TKTNARTRKGPKRTVAGKKKAGK"
     gene            complement(16042..16155)
                     /gene="rpmJ"
                     /locus_tag="PLK64_01610"
     CDS             complement(16042..16155)
                     /gene="rpmJ"
                     /locus_tag="PLK64_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006042225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPV78466.1"
                     /translation="MKVQPSVKKICDKCKVIRRNGRVMVICENLRHKQRQG"
     gene            complement(16274..16495)
                     /gene="infA"
                     /locus_tag="PLK64_01615"
     CDS             complement(16274..16495)
                     /gene="infA"
                     /locus_tag="PLK64_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPV78467.1"
                     /translation="MAKKDGVIEIEGTIVEALPNATFRVELSNGHKVLAHISGKMRQH
                     YIRILPEDRVVVELSPYDLSRGRIVFRYR"
     gene            complement(16664..17050)
                     /locus_tag="PLK64_01620"
     CDS             complement(16664..17050)
                     /locus_tag="PLK64_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HPV78468.1"
                     /translation="MGMVTDYLATVDAARRPALERVVARAHDLVPGLVEGTAYGMPVL
                     LHRGKPLLSAVEAKAHLALYPHSGVIVDLVAADLDGFSLSKGTIRFQTDQPIPDAVLD
                     RVITLRRQHIDEALDTPRPKRARGNA"
     gene            complement(17063..17908)
                     /gene="map"
                     /locus_tag="PLK64_01625"
     CDS             complement(17063..17908)
                     /gene="map"
                     /locus_tag="PLK64_01625"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPV78469.1"
                     /translation="MSARRKTPGRTDDQLLLMRAAGLLVGQTLEVVAAAVADGVTTLE
                     LDRLAEAHIRDHGGRPSFPEVPGYRHTLCTSVNEQIVHGIPGERALRAGDLVSIDCGA
                     IVAGWHGDAAVTVIVGGVEAARPEDLALSRATEDSLYAGIAALRVGERLYAVGEAVEA
                     CIEAAAAANGQTYGIVEDYVGHTIGTEMHQDPQIFNYAVRTRGPMVRSGYTGAIEPMI
                     TLGSAESDVLADGWTVVTSDGSRAAHWEHTVAVRDEGLWVLTAIDGGAARLAALGAAF
                     APLSD"
     gene            complement(17913..18485)
                     /locus_tag="PLK64_01630"
     CDS             complement(17913..18485)
                     /locus_tag="PLK64_01630"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPV78470.1"
                     /translation="MRLVIMGPPGAGKGTQAERIAEQFGIPAISTGAIFRANVKGQTE
                     LGKQVEAIMATGGYVPDELTNALVRDRLAEADCAPGFLLDGYPRTVGQLHTLDAHLEE
                     TGRQIDAALELVVDEDAMVARLLARAADQGRADDTEEVIRERMSIYRAATAPLTAIYG
                     DQGKLRQVDGMGDIDEITARIVAALADLQG"
     gene            complement(18482..19798)
                     /gene="secY"
                     /locus_tag="PLK64_01635"
     CDS             complement(18482..19798)
                     /gene="secY"
                     /locus_tag="PLK64_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPV78471.1"
                     /translation="MLTAVSRAFRTPDLRKKLLFTLLIMAIFRLGSAIPTPGVSYTKV
                     AQCLAGADPAQGFIGLVNLFSGGALLQLSIFALGIMPYITATIIVQLLTVVIPRFEAL
                     KKEGQAGQTKMTQYSRYLTIGLAILQSATLITFARNPSQIFGPNCTEILTDTNVSTIL
                     MMVLTLTAGTGLIMWLGELITDRGIGNGMSLLIFTSIAAGFPSSLWAIQQQEGRWDVF
                     IGVILVGFFVMALVIFVEQSQRRIPVQYAKRQVGRRMYGGSSTYIPLKVNMAGVIPVI
                     FASSLLALPALVAQFATPQDGSLPPDWVIFVQSNLVQGDSALYMAVYVALILFFTFFY
                     VSITFNPVEVADNMKRYGGFIPGIRAGRPTAEYLDYVLTRITVPGAIYLAAISLIPLI
                     ALKLFNANQNFPFGGTSILIIVGVGLETVKQIQAQLQQRNYEGFLR"
     gene            complement(19969..20451)
                     /gene="rplO"
                     /locus_tag="PLK64_01640"
     CDS             complement(19969..20451)
                     /gene="rplO"
                     /locus_tag="PLK64_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPV78472.1"
                     /translation="MAKSTVSTSDTASGTTLKVHHLRPAPGAKTPKTRVGRGEGSKGK
                     TAGRGTKGTKARYQVSEAFEGGQMPMHMRMPKLRGFKNPFRTVYQVVNLDRIGALYPE
                     GGEITVADLVAKGAVRDGQLVKVLGTGEISVKVNITADAFSTSAKDKIEAAGGSVSQA
                     "
     gene            complement(20454..20636)
                     /gene="rpmD"
                     /locus_tag="PLK64_01645"
     CDS             complement(20454..20636)
                     /gene="rpmD"
                     /locus_tag="PLK64_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HPV78473.1"
                     /translation="MARLKVTQTRGHVGQKRFARETLRSLGLKRIGDTVVKEDRPEIR
                     GMVRAVTHLVAVEEVD"
     gene            complement(20636..21316)
                     /gene="rpsE"
                     /locus_tag="PLK64_01650"
     CDS             complement(20636..21316)
                     /gene="rpsE"
                     /locus_tag="PLK64_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPV78474.1"
                     /translation="MPGPQRRGTGAGAGTAAGGESGERRGRGGRDGRDGRRDSREQEK
                     SQYLERVVTINRVAKVVKGGRRFSFTALVVVGDGDGTVGVGYGKAKEVPAAIAKGVEE
                     AKKAFFKVPRNQGTIPHPVQGEAAAGVVLLRPAAPGTGVIAGGPVRAVLECAGIHDVL
                     SKSLGSSNAINIVHATVAALRQLERPESVAARRGKAVEDVAPAAMLRAQAAAAAKSAA
                     AKADKVGA"
     gene            complement(21360..21734)
                     /gene="rplR"
                     /locus_tag="PLK64_01655"
     CDS             complement(21360..21734)
                     /gene="rplR"
                     /locus_tag="PLK64_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPV78475.1"
                     /translation="MAMIIKRGKSKAAARIRRQVRGRKKISGSPERPRLVVSRSTRHL
                     FAQIVDDTAGKTLACASTMESDLRAFDGDKTAKAKKVGELIAERAKAAGVEAVVFDRG
                     GNKYHGRIAAIADGAREGGLSL"
     gene            complement(21738..22280)
                     /gene="rplF"
                     /locus_tag="PLK64_01660"
     CDS             complement(21738..22280)
                     /gene="rplF"
                     /locus_tag="PLK64_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPV78476.1"
                     /translation="MSRIGRLPVVVPAGVDIAIDGQAVTVKGPKGQLALVVAEPIAVA
                     KGESGAVEVTRPDDERLSRSLHGLTRTLIANMVQGVTAGYEKKMEIVGTGYRVQAKGA
                     NLEFALGYSHSITVEPPAGISFVVEAPTRFSVQGIDKQQVGEVAANIRKLRKPDPYKA
                     KGIRYAGEQIRRKVGKAGKK"
     gene            complement(22293..22691)
                     /gene="rpsH"
                     /locus_tag="PLK64_01665"
     CDS             complement(22293..22691)
                     /gene="rpsH"
                     /locus_tag="PLK64_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPV78477.1"
                     /translation="MTMTDPIADMLTRVRNANSAHHDSVAMPYSKLKANIAEILKREG
                     YIGSWAVSDAEVGKTLTITLKYGPNRERSIAGVRRVSKPGLRVYAKSTNLPRVLGGLG
                     VAIISTSTGLLTDKQAHEKGVGGEVLAYVW"
     gene            complement(22840..23025)
                     /locus_tag="PLK64_01670"
     CDS             complement(22840..23025)
                     /locus_tag="PLK64_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HPV78478.1"
                     /translation="MAKTALVNKANATPKFKVRAYTRCQKCGRPHSVYRKFGLCRICL
                     REMAHRGELPGVTKSSW"
     gene            complement(23027..>23168)
                     /locus_tag="PLK64_01675"
     CDS             complement(23027..>23168)
                     /locus_tag="PLK64_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPV78479.1"
                     /translation="QSMFHEIDQDRIDRVRGMDITVVTTATTDDEGRALLKALGFPFK
                     EN"
BASE COUNT         3485 a         7973 c         8040 g         3670 t
ORIGIN      
        1 cgcagccctg gtcgcgcaac gctcccacct catcgccgac cacatcgccg ccgggtcgct
       61 cggcatcatc ggcgcgacct acaacctgcg cgacggccaa gcaggtgtgg tcaccgcgat
      121 cgggatcgac gtcacctgag ccgcacggca taccgcaggg gtatgccgcc tcgccccctc
      181 agcgctgcag ccgcctcagg cgtcgctcaa cgacaactgc gacttgacca cgtcggccag
      241 gcggtcggcg atcgcctggg cctgatggtg atcggcagcc tcgaccatga cccgcacgag
      301 cggctcggtg cccgacttgc gcagcaacac ccggccggac ccgtcgagct ctcgctgcgc
      361 ctcggccacc gccgactgca ccgtggggtg accgtcgacc gcgtccttgt cgacgttctt
      421 gacgttgacg agcacttggg gcaggcgggt catcacggcc gccaactcag ccagcgggtg
      481 cccggtctgg gcgacccgcg aggccagcat cagcccggtg aggacgccgt ccccggtcgt
      541 cccgtggtcg aggaagatga cgtggccgga ctgctcaccc ccgagggagt agccgccggc
      601 cttcatgtcc tccaggacgt aacggtcgcc gactccggtc tgtcgcaccg tgattccctc
      661 acgggtcatg gcctgcaaca tgccaaggtt gctcatcacg gtggccacca gggtgtcgtg
      721 ggccagggtg ccgcgctcgc gcatggccat cgcgaggatg cacatgatct ggtcgccgtc
      781 gacgacctcg ccgcgcgcgt ccactgccag gcatcggtcg gcgtcgccgt cgtgagcgat
      841 cccgagctgg gcgccgttct cgatgacggc gcgctgcaac cgggccagat gggtggaccc
      901 atagcccgcg ttgatgttct cgccgttggg ctccgcgccg atgaccgtga cgaacgcccc
      961 ggcctcacgg aaggcccagg gcgacaccat ggaggcggcg ccatgggcgg cgtcgatgac
     1021 gacgtgcagt ccgtcgaggc gatgcgggag cgtcgtcagc aggtggcgga catacctcgt
     1081 ggcgccgtcc tcgagccgac ggatccggcc cacggcagca ccggtcgggc gcgtccaagg
     1141 ctcctgcatc cgcgcctcga tggcgtcctc gacgacgtcg tcgagcttct gtccgccgcg
     1201 cgcgaagaac ttgatcccgt tgtccggcat ggggttgtgc gaggccgaga gcatgacccc
     1261 caggtcagcg cccaggtcgg ccgtgaggaa ggcgatcgcc ggggtgggca gcagccctgc
     1321 gtccatgacg tcgacgccgg cagacgccag accggccatc accgcggcgg agaggaactc
     1381 acccgacgcg cgcccatccg tcccgacgac cgcgaacgga cggtgccccg cgaactcgcc
     1441 ggcatcaccg agcacgtgcg cagcagccac cgccaggtcc agggccaact cggccgtgat
     1501 gtcctggttg gccaggccgc gcacaccatc ggtcccgaat agacgtccca cgagtgttcc
     1561 tttcaccagc aatcccgtat gccgcgtatg ccgcgtcgcg aggtgcgcgg gcggcatacc
     1621 ccgaagcaac ctactcctgc cccacgtgag caagacgcac gacgggcggc ccgggttgcc
     1681 ccgagccgcc cgtcggcggt cacagtcgtg acatcaacgc ttgctgtact gcggtgcctt
     1741 gcgggccttc ttgagaccgg ccttcttgcg ctcggggacg cgggcgtccc gggtgaggaa
     1801 gccggccttc ttcagcgtcg ggcggttgag ctcctcatcg accccgttga gggagcgcgc
     1861 gacaccgagg cggaccgcgc cggcctggcc ggaggggcca ccgccgtgaa cccgcacgag
     1921 gacgtcgtag gcgccgtcga gctcaaggac cttgagcggc tcgttgacga tctgctggtg
     1981 gaccttgttg gggaagtagg agtccagcgt gcggccgttg atggtccact cgccggtgcc
     2041 gggaacgatc cggacccggg cgatggcctg cttgcgacgg ccggtggcag caccgggagc
     2101 cgaggcggac ggcttgcggg cagccgcgcc gaccggggcg ttctgggtct cggtcgtgta
     2161 ttccgagagc tcgggctcgt cgacgtcgag cgcgacgtcg gtcgtgttgt cagccattac
     2221 tgggccacct gggtgatctc gaagggcacg ggcttctgtg ccgcgtgggg gtggacgtcg
     2281 ccggtgtaga ccttgagctt ggtcagctgc tggcgagcga gggagttctt ggggagcatg
     2341 ccgcgcacgg ccttctcgat ggcccggcgg gggtccttgt cgagcagttc ggcgtaggac
     2401 atcgaggaca gaccacccgg gaagcccgag tgccggtagg cccgcttgag ctgggccttg
     2461 ttgccggtga gggcaacctt ctcggcgttg atgatgatga cgaagtcgcc ggtgtcgacg
     2521 tgcggcgcga aggtcggctt gtgcttgccg cgcagcagca cggcggtctg gctggccaga
     2581 cgaccgagca cgacgtccgt cgcgtcgatg acgagccagg cgcgggtgac ctcgccgggc
     2641 ttgggggtgt aggtgcgcat gtgcgtcact ttccgtttcg ggttcacagc ttcggggtat
     2701 gccgtgtgcc tggcaccgcg gccggctccc cctcgcgggt ggtccgggtc ggtgtggcac
     2761 ccacccggca taccgccgcg cgtggcacaa cagtcctcaa gtctaccggg cggacgggcc
     2821 aggccctatt cggcgtggat gggccgctga cctgcgacga tgccgccctg actggcgtgg
     2881 cgtcagagtg tgcaaagaca gtgtgcaaaa cttgtgtgca tgctcggttt cgccccattg
     2941 acccccgacc agatccgggt cctggcccat cccctgcgct cccgcttggt cgaccggctg
     3001 cgtcggcgcg ggcccgcgac cgccaccacc ctagccaagg agctcgacac caactcggga
     3061 gcgacgagtt atcacctacg ccggctcgcg gcatacgggc tggtccaacc tgcgggaggc
     3121 gggcgcggac gggagcggcg ctgggaggcg agcgcgcggc ggcataccct gccgggcggc
     3181 gccgacgcag gcgttggtgg gtcgggcggg ggtgccagtg gaccgatcgc gtcgacccgc
     3241 ggcgccgacc ctgacaccga gtcgagcctg gactggctcg accgagacta cgtgcggcac
     3301 ttcgccgaga aggccgagct ctggctcgac gccgccccgg agtggccggc gggctggacc
     3361 gacccctgcg gcctgcgcga cgggatggta ctggtcaccg caccacaact ggccgatctg
     3421 cgagccgagc tcgacggggt gctggcgcgc taccgccggg tcgggcaggg caaccccaag
     3481 gcgaagcggg ttgccgttta cacctcctgc cagcccctgg atctggactc cccgcccctg
     3541 accccctagg ggaacgacgc tgtccgaggg ggagggtcag gaggcggccc cgtcgtggct
     3601 cggatcagcc gacgaaggcg tcgacggccg ggacgacgta ctgcagttcc cgggcagcgg
     3661 cggccgcgat gtccgggccg acgaatcgac cgaccaggtg gagggcgacg tccagggcac
     3721 acgcgggggc cccgccggtg acgacgtctc catcatcgac gacgcgggcc tcgagaaccg
     3781 tcacccccgc gaacgcccgc aagtcatcga gcgcgtgggg gttggtcgtc gcccgatgcc
     3841 cggcgagcac acccgcagcg gccaggatca gcgctcccgt gcagaccgag gcgacaacgg
     3901 tccccgcggc gtgggccgcg gcgatcgctc ggggcacctc gccagcctgc acgagccccc
     3961 acgccccggt cggcgagcga tcaagccagc cgccaccggg aacccacagc agatccgggc
     4021 gctcagacag cgcgccatgg gccatgacca cggctccgtg atcgccgcgc accgggcctg
     4081 cgccgtcgag cgtgacgaac gaggcacgga cccgatcggt gacctgggcc aggccggcaa
     4141 acacctccca gggcgccatt gcatcaaact cgtcgaagcc gtcaaagagc aggatctcga
     4201 tgttcatgcc ccccatcctc ggcccggtcg atgctctccc gggagtggct ggaaagacat
     4261 tctgcgatag ttttgagcca tggctgacac tgcagtccag acagcgcggc accgcgtcgt
     4321 cggcctgctt gtcggtgagg tcgtcatgtt tgacctggcc atcgccgacc aggtgttcgg
     4381 gcgcaccgac gggtacacct ggagtgtttg cggagtgcgt cgtgggtggc tgcccaccac
     4441 caccgggctg gccgtgcgcg tcgaccacgg gctggcggcg ctcgacgagg ccgacacgat
     4501 cgtcgtcccg ggaatcggcg acgaggcctg gccggtcccc gctgcggcgc tgcgcaggct
     4561 gcgcgccgcg cacgcccggg gggcccgagt gatctccgtc tgcaccggtg ccttcgtgct
     4621 cgcggctgcc gggcttctgg atggtcgacg cgcaacgacg cactggcgct atgcggggct
     4681 gctggcgagc tctttccctg cggtcgaggt ggacccggcg gtgctctatg tcgatcacgg
     4741 gaccatcgcc acgtcagcgg gtctggcggc ggggatcgac ctgtgcctgc acctggtcgc
     4801 ccgggatctg ggggccgatc gcgcggcggc cgtggccagg aacatggtcg ttccgccgca
     4861 ccggcgagga ggtcaggcgc agttcgtcga ggcactgagc gcgacgcacg aaggcaccgg
     4921 actcgccgag gtgttggact ttatgaccgt ccggtgcgcc gagccgctca ctctggccga
     4981 cctggctcgc cgggccgcga tgagcccgcg gacgtttacc cggcacttcc gagcgcagac
     5041 cggagtctca cccaagcagt ggctgctggc gcgcagactg gaggcggctc gtcgcctgct
     5101 ggagtcgagc gacctgtcgg tcgccgagat cgccgtccgc acgggcttcg ggacaccgct
     5161 cgccctacgg cagcagttca cccgggtgct gcggacgacg ccaacggcct accgccgcac
     5221 gttcgccggc taaaccgcgc cggctgaacc gctccgatcg atcacgctgt cggccgatcg
     5281 tgctgtcggt cgtttgtggt tcagcgggcc cggtcgacgc ggccttcgtc ccagaccggg
     5341 tcgaccgact cgatgacccg gccgtcggcg cggaacacga ggaaccggtc gaagccgcga
     5401 gcgaaccacc ggtcgtgggt cacggccacg acggtccctt cgaaggcgtc cagggcggcc
     5461 tcaagcgcct cggccgagtc caggtcgagg ttgtcggtcg gctcgtcgag cagcagcatg
     5521 gtggcgccgg acaactcgag cagcaggatc tggaaccgcg cctgctgccc acccgagagt
     5581 gactcgaagg tctgctccgc cgccttggcc aggccgtaac ggtcaagcac cttggccgcc
     5641 tcctcgcggc cgcgcccgtc gcggtgttcg tcgccgcgat gcaggatctc ggcgagggtc
     5701 cgcccgagca gcgcgggttg gtggtgggtc tgggcgaacc agcccggccg cacccgggcg
     5761 cccaaccggg ccgagccggt atgccgcacc ggctccggtc gtgtctcccc cacgggctgg
     5821 tgctccacgt cggggtcaga cccaccgcag gcgagcaacc gcaggaagtg cgacttgcca
     5881 gagccgttgg agccgagcac ggccaaccgc tcgccgaacc agatctcgga gtcaaacggc
     5941 tgcatcagcc cggtgagctc aagccgctcg cagacgatcg cccgcttggc ggtccggccc
     6001 cccgtcagcc gcatccgcag gcgctgggtg accggcaccg cctcgggcgg ccctgcctcg
     6061 aggaaccgac gcagccgggt ctcggcggcc tgcaacttgg gcgccatgtc ggagttgtag
     6121 gccgccttct ggcgatacat gacgacgagc gccttgagct tgatgtgctc ctcgtcccac
     6181 cgccgtcgca gctcttccaa gcgcgagttg cgatcctcgc gagcagcggc ataggtgccg
     6241 aaggggccag gatgcaccca caacgtcgag cctgcggcac ccggctccag ggtggcgacc
     6301 ctcgtcgcga cccgggcgag caactcgcgg tcgtgtgaga tgaacagcac cgtcttgggt
     6361 gaggcgatga gcgcgtcctc gagccaccgt ttgccgggca cgtcgaggta gttgtccggc
     6421 tcgtcgagca gcagcacctc ctcagggccg cgcaggagcg cttcgagcac cactcgcttc
     6481 tgctcgccgc cggacagtgt tgtaacaggc cgccattggg cccgctcgta gggcaccccg
     6541 agggccgcca ccgtgcaggt gtcccacagc gtctcggcct cgtaaccccc tgcgtcaccc
     6601 cagtcgacaa gggcctgggc gtaggcgagt tggtcccgct cggtgtcgtg ctccatgacc
     6661 gccaactcgg tgcggtcgat ctcggcggcc gcggcgcgta ccttgtcgga agcgaccgag
     6721 aggagcaggt cgcgcaccgt cgactcgtcc cgcaccgagc cgatgaactg ccgcatcacc
     6781 cccagcccac ccgagcgggt cacggccccg gaatgcgccg ccaggtcgcc gctgatgatc
     6841 cgggacaggg tggtcttccc ggagccgttc ggaccgacca gcgcgatccg ggcgccttcg
     6901 ccgacgcgca ggctcacttc ccgcagcagc ggccttccgt ccggaaggtc gaagctcacc
     6961 tggttgacgt cgacgtgtcc cacaacgcgg cagtctcccg tatgccgccc gcccccgtca
     7021 tctcggtttc gtcagcgttg gggggccgac cctcaggctg acgcctcact cggcggcgcc
     7081 gacgcgtagg cccgagagag ttgccggtag ggccgggacg ccagggcaag cagcacggtg
     7141 accagcatga tcgccccggc cagcaggaac accagggcga tgccgcgggc ctcgcccgcg
     7201 ccgagcagcc agccgaatcg ttccttcccg gcatcggagg ccatgtaggg aatgagccag
     7261 aactgggcga tcggcccgat gaggaaggcc gagacggggc tggccgcagc ctcgacactc
     7321 tgtgcgaagc cgaacacccg cccctgggtc cgatagggca cgactcgctg caggatcgtc
     7381 tgctcagccg cttcggcgat cgggatgacg cacatgtaga gcaggatccc gccggcatag
     7441 agccaccaga cctcgcggat ggtgaacgcc atcccgagga ctgcggccac gacgttggcc
     7501 agcatcagcg agcgcagcgg tgtcgcgccc agtccgaact tggcgaccca cgccccgccg
     7561 atgatgaaac cgatgctcgt gacccccagc acgatgcccc aactctcgac ggagaacagg
     7621 gtcagcccgt aagggtccat cagcgccatg aacacgccgc cgacgaggtt gttgaacgtc
     7681 gcgaacagca cgagggccat caagccgggg acggcgcgga tcgcctcgag ggcgctgcgg
     7741 acgtcgaagt gccccgctga gtcactctcg tgccgggtcg gcggaacctc agggataggg
     7801 attgtcagga ggtgcccgag ggacagggcg gtgagcccga tcgcgatgac caacgtccac
     7861 cccatgccca gcaggccgat cgacaagcca ctgaacacgc tggtcaccat gaacgccagc
     7921 ccttggaccg tgcccacgag accgttggcc ttgtcgcggt cgtcggccgg gatgagcagg
     7981 gtgaccgtcg tcgacagcgc aatgttgcgc atgctctcga ccaccccgcc gacgaggatg
     8041 acgaggacga agacccagaa gaccgggccg ttccagtcga tgagtcgctc ggtggggaag
     8101 gcgaggaaca acccgcccgc cgccacgaag gtcacgagcg tcacgaggtt ggagccgatc
     8161 atgacgttct tcttgacgtg ccggtcgacc acgctcccga acaccgtccc gaacacggcg
     8221 atgagcagca tgaacgaccc gccgatgatc gacgtcgcga ggaccgacct ggtctccaga
     8281 taagcccaga acgtcagggc gaaccacagg taggacgtgg tcacgttggc gatcagggtg
     8341 ttgagcagga cgaaccggaa cgcccgcacc gtggccgggc cgcgctccac accgggcggt
     8401 aacaacactt ccgaagtcat cgggacaccc taacgacagc cgctgacacc cctaggcgcc
     8461 gtcgcgtcgg gcccgggact cgcgagcgcg gtcggccagg gccgcatcgg cgggatagcc
     8521 gacttcctcc aggcagaggc cgtgcgccgg catgaccttg acgcggccgt tgcgggccgc
     8581 cgccgcctgc acctgccccg gccaagccgc gtcctcccga ccgagcccca ccggcacgac
     8641 cgaccccacc agcgagcgca ccatggagtg gcagaacgcg tccgccacga cccgggcctc
     8701 gacgacaccg tccgctcccc gagcccagga gaagtccagc aaggtccgca ccgtggtggc
     8761 cccggcccgg cgtttgcaga aggccgcaaa gtccttgagc cccagcagcc cagcactcgc
     8821 ctggttcatc acgtcgacgt cgaccggatc gggcagccac accgtgtcac gccgccggac
     8881 cgggtcggcc gtcgagagcg agtcggcgat ccgatacgag tagcgccgcc acaccgccgc
     8941 gaagcgagcg tcgaaaccgg cgggtgcgcg gcatacccga tgaacgagga tgtcggagcc
     9001 gagcagcccg cgcagccgca ccaccgccgc ctcctccggc gaccggcgtg agcgcccgac
     9061 gacggcgagg tatgccgcct cgtccacgtc ggcgtgcacc acctgaccac gggcgtgaac
     9121 ccccgcgtcg gtgcgaccgg caacggtgag ccgaaccggc tcgggtgcgc gcagcagggt
     9181 ggtcagggcc ctggacaact caccctcgac ggtccgctgc ccaggctggg cggcccaccc
     9241 cgcgaagtcg gtcccgtcat agcccaggtc gaggcgcact cgcacgctca tcggtcacca
     9301 cgtcccgagg ccggccgccg caggcagatc aacgcacaca cgcggaccat catcgcgtat
     9361 gccgtgcgcg gactagcgtg gacctatgcc cacctggacc atggcattcg gcgatccggg
     9421 gctggatctg cccactggcg gcggcaaggc cgtcaacctc gggcggatga cccggtcagg
     9481 gttcccggtg cccgacggat tcgtcgtcac caccaccgcc tatcgccacg cggtggccgc
     9541 gaccgccgga ctggccgacc tcatcgacgc tgccctcgcc gagggcgacc ccacaggccc
     9601 ggcgacggcc gagtcggcct cccgcgcgat ccgggccgcg ttccgagccg cgcctgtccc
     9661 ggcggaggta gccgaggcgg tgcgcgcggc atacctcgcc ctcgatgagg ggccggtggc
     9721 ggtgcggtcc tccgcgacgg ccgaggatct gccggacctg tccttcgccg gtcagcagga
     9781 cacctacctc gggatcgtcg gcgaggccgc ggtcctcgac gccattcgag actgctggtc
     9841 cagcctgtgg accccgcgag ccatcgtcta tcgacgccgc aacctggtcc ccgatgccgc
     9901 cgccgccctg gcggtcgtca tccagcgact cgtccccgcc gacgtctccg gcgtcctgtt
     9961 caccgccaac ccgctcaccg ggcaccgcgg ccagatggtt ctcgacgcga ccttcggcct
    10021 cggcgaggcg ctcgtgtccg gccaggtcga gcccgaccac gtggtcgccg atgccgccag
    10081 cgggcgagtg ctcagccgca ccgtcggcgc caaggcggtc gccaccgtcg tctcgcccac
    10141 cggtggtgtc accaccgaga cccgccaggc ctccggagaa tccgccgtca ccgacagcca
    10201 ggtcgccgcc ctcgtcgcgc tcgggcgccg ggtgcaggcc gagtatgccg ctccccaaga
    10261 catcgagtgg gccatccacg acgggacgat ccatctccta cagacccggg cgatcacctc
    10321 gctctacccc gtcccggacg gggcgccacc ggactcggtg tggctgtcct tcggtgccgt
    10381 ccaaggcatg ctcgccccga tgactcccct cggtcgcgac gccatccggt gcatcctctc
    10441 cggaggtgcg agcgtcttcg ggcacaccgt cgacgagcgg accaacccct atctcggggt
    10501 ggcgggcgag cggttgtgga ttcggctgga tcggctgctg cgcaaccccc tcggccgacg
    10561 cggcgccccg gagttcctgc ggttcgtcga tcccagcgcc gcagcaatcg ccgacacgtt
    10621 gcttggcgag cactggctgc gccccctgcc ccgagcgacc tcccggcgcg tcgccgcgca
    10681 gaccgcgcga gacctggccc ggttcggtcg agtggcagtg ccgcggatcg cgcgagccct
    10741 cgctcgaccg ctcccggcgc gcgaccgctt cgacgatgac attgacgacc tgatcgccac
    10801 cgctcgcgaa cgcgaagccg atgcggcggc gcaggccgag ccggtggccc ggctggcagc
    10861 ccgcgcccgg gccctgcgag ccgacctgcg gggtgcgttc ccgaccctgt tgcccgacgc
    10921 ggcgccgctc gtggctccgg ccgtcgtcat gctgcgggtg ctctccgaac tcaccgatgc
    10981 cacgaccgac gggcacgggg tgtcgcccct agccatggaa gtcacccggg ccatcccccg
    11041 caaccccacg accgagatgg acctcacgct gtgggcggtg gccgaggcga tccgcgccga
    11101 ccccgacgcg accttcgcct tccaagcgct cactccgacc gaactcgccg ctcggtatgc
    11161 cgcccgccgc ctccccgacg gcgcccaggc cgccctcgat gccttcctca cggcatacgg
    11221 gatgcgcggg gtgggcgaga tcgacctcgg gcgaccgcgc tggcgtgagg acccgaccga
    11281 cctcctgcag acgctgacgg tctacctcga cctccccgag gacgccgctc cgcccgcggt
    11341 cttccggcgg ggcgagcagg cggcggcggt cgccgtcgag cgcctggccg ccgtggcatc
    11401 cggtatgccg cgtggtcacc tccgcgcgcg caccgtgcgg tggctcgcct cgcggatccg
    11461 agccctggcc ggcggccgcg agcagccgaa gttcgccgtc gtgcaggtca tgggcgtcat
    11521 ccgtgagggg ctgctggcca gcggtgccga cctcgtcgcc ctcggggtcc tcgaccggcc
    11581 cgacgatgtc ttcttcctgc acctgagcga gttgaccggt ctcacccagg cctcgcttgg
    11641 tggcctccgc cccgcggtcg aggcccgtcg ggccgcgtgg agcaccgaga tccgacgggc
    11701 ccgggtgccg cgggtgctcg tcggtgacgg acgggcgttc cacgagggcg tcgctcggac
    11761 agccgacctc gccgatctgc ctcccggcac cctcgttggc agtccggtct cccccggcgt
    11821 cgtcgagggg ctggtccggg tggtgcacga tccggcgccg tcgggcctgc agcccggcga
    11881 gatcctcgtc tgcctaggga ccgacccggc gtggaccccc ctgttcctca cggccggcgg
    11941 gctgatcacg gaggtgggcg gcatgatgac gcatgggtcg gtcgtcgccc gcgaatacgg
    12001 catacccgct gtcgttggag tcgacgcggc cacgactcgc ctagtgaccg ggcagcggat
    12061 ccgggtcaac ggatcggtcg gaaccatcga gctgctcgag gactgaccct cccccacccg
    12121 cacccaaaca cccgcaccgc aacacgccgc cgctacatgg caaggccccc gtccgagtgg
    12181 acgggggcct tgccgttgct ggagccggtc acccggcgac cagatcagtc ctcgctcttg
    12241 gacccggcct tcttgaagcc ggcagcctca gcgtcggcgg cggtcttgaa ccagaactcc
    12301 gcgacggttg cgtcaaacca acggccatcc ggctcgtggt agagacccga gtcggcgttg
    12361 cccttgaccg tgtagccctc gggagccgag ccgtccgcca gcggcgccgc agcaccggcc
    12421 ggcaactcgg cagcctccgt ggccgcagcc tcgggggcgt cgaccagacc ggcaacctcg
    12481 gtcgccggcg cggcctcgac cgcgtcgtcg atctcgaggt ccttcggagc ttcgacggca
    12541 gcctcggtcg cggcagcggc cttcttcttg tcggcagcgg cacgcttggt cgcggcctcg
    12601 gcctccttga cggtggcctt cttcgccgac agcggctcgc gaaccaactc gatcacggcc
    12661 atgggggcgt tgtcgccctt gcgcggcccg atcttgatga tccgggtgta accgccctcg
    12721 cgctgggcca tgtcgggcgc gacctcggtg aagaggcggt gcacgacgcc cttgtcacgg
    12781 acgacggtga ggacccgacg acgcgcgtgc aggtcgccac gcttggcgaa ggtgaccatg
    12841 cgctcggcca ggggacgcag ccgcttggcc ttggcctcgg tcgtggtgat ccggtcgtgc
    12901 tcgaacagtg cggtggccag acccgccagc agcagccgct cgtgtgccgg cccgccaccg
    12961 aggcggggac ccttggaagg ggtaggcatt gtttctcctt ggttgtgcgt ccggtatgcc
    13021 gtgtcgcgcg gtccgcgctc ccggcacccg gtctgcggct agggggtcag aactgctcgt
    13081 cctcggccag cgagcgacct tcgtcgaggt cgtcgtagtc gtcggcgtag gtgtcggcga
    13141 tcgaggccgg gtcgaatccg ggcgggctgt ccttgagggt cagacccata ccggcgagct
    13201 tggccttgac ctcgtcgatc gacttggcac cgaagttgcg gatgtccagc aggtcggcct
    13261 cgctgcgccc aacgagctca cccacggtgt ggatgccctc gcgcttgagg cagttgtagg
    13321 accggaccgt gaggtcgagg tcctcgatcg gcagggccag gtcggcagcc agcgcggcgt
    13381 cggtgggcga cgggcccatg tcgatgccct cggcctcgac gttgagctcg cgtgccagac
    13441 cgaacagctc gacgagcgtc ttgccggccg aggccatggc gtcgcgcggg gtcatcgagg
    13501 acttggtctc gacgtcgacg atgagccggt cgaagtcggt gcgctgctca acacgggtcg
    13561 cctcgacctt gtaggtcacg gcgagcaccg gcgagtagat cgagtcgacc gggatgcggc
    13621 cgatctcctg gtcaccggac ttgttttgct gcgccgagac gtagccacgg ccacgctcga
    13681 ccgtcagctc catctcgatg cggcccttgg cgttgagcgt ggccaggtgc agatcggggt
    13741 tgtggacctc gacgccggcc ggcggcgcga tgtcggcagc cgtgaccgcg cccgggccct
    13801 gcttgcgcag gtacatgacg acgggctcgt cgtgctccga ggaaacgacg aggctcttga
    13861 tgttgaggat gatctcggtg acgtcttcct tgcagcccgg aatcgtctgg aactcgtgca
    13921 ggacaccgtc gacgcggatc gaggtgaccg ccgcgccggg gatgctcgac aggagcgtgc
    13981 ggcgcatgga gttgccgagg gtgtacccga aacccggctc gagcggctcg atgacgaagc
    14041 gcgaccggtt gtcggccacc gtctcttcgc tgagcacagg acgctgtgcg atgagcatgt
    14101 tctttccttc ccgcgagcga ccgctatttg acgctcacgt atgcgcacgg catacctctg
    14161 tctgtatgcc gtgaggcccc tggccggtat gccgcgggcg agcccgtgag gggcgcccgt
    14221 ggtgggctcg gagcgagtcc gagccacccg gcataccgtc gccagggaac cacgaggggc
    14281 cgaaacccct cgtggcgggg gtcagttctt ggagtagagc tcgacgatca gctgctcggt
    14341 gaggatcgtg tcgatctgct cacggacggg caactggtgg atgagcacgg tcagcgagcc
    14401 gtcgacgacc tgcatccacg ccgggggcgt gcggtcaccg aaggtctggc gagccagctc
    14461 gaacgggaac gtctcgaccg actgcttgcg caccgtgatg atgtcgaact gctcgacccg
    14521 gtaggacggg acgtcgacgc ggtggccgtt gacctcgaag tggccgtgcg acaccaactg
    14581 gcgcgactgg cgacgcgtgc gcgccaagcc ggcccggtag atgacgttgt ccaggcggga
    14641 ttcgaggatc tgcagcaggt tgtcaccggt cttgccgggg cgggtggccg cctcctggta
    14701 gtaacggcgg aactgcttct ccatgacgcc gtaggtgaag cgggccttct gcttctcctg
    14761 cagctgagtg aggtactcct tctccgtgat ccggcgacgg ccgtgctggc cgggcgggaa
    14821 gggacgcatc tcgaagttct tgtcgccgcc gacgaggtcg accttgaggc gacgcgactt
    14881 cttggtgatg gggccggtat agcgagccat gatctatctg tctcctggtc ctgatccggc
    14941 gctcagacgc gacgacgctt gggcgggcgg acgccgttgt gcggcgaggg cgtgacgtcg
    15001 ctgatcgcac cgacctccag gccggtggcg gtcagggaac ggatggcggt ctcgcgaccg
    15061 gatccggggc ccttgacgaa gacgtcgacc ttgcgcatgc cgtgctccat ggcacgacgg
    15121 gcagcggcct cggccgccat ctgcgccgcg aacggggtgg acttacgcga gcccttgaag
    15181 ccgacctggc cggcagaggc ccatgagatc actgcgcccg tggggtcagt gatcgagatg
    15241 atcgtgttgt tgaacgtgct cttgatgtga gcatgcccgt gggcaacgtt cttcttctcc
    15301 ttgcgccgaa ccttcttggc ggcgccctga gcagtcctgc tcttgggagg cattgttctc
    15361 ctacgtcctt aggtatgggg gctggcaacc cgtcggggtt acttgccggc cttcttcttg
    15421 ccggcgacgg tgcgcttggg acccttacgg gtacgcgcgt tggtcttggt gcgctgcccg
    15481 cgaacaggca ggccacgacg gtgccgaatt ccctcgtagg agccaatctc gactttgcga
    15541 cggatatccg cggcgacctc acggcggagg tcaccttcca tcttgacgtt ggtctcgagc
    15601 cagtcacgca acgagacgag gtctgcgtcg gtgaggttct tgacccgggt gtccgggctg
    15661 atcccggtcg ccacgagggc cttgctcgcg gtagtgcgac ccactccgaa gatgtaggtc
    15721 aggccgatct ccacgcgctt gtcacgcggg agatccacgc cgatgagacg tgccatctgg
    15781 tgctgtgctc ctgatcggtt gcggaggtct ggtgcagcac cgatccggta tgccgtgcgc
    15841 gtcgccctcg cgggtctgcg ctggtccggt ccccggcctc cgtgccgggg gtgacgtcct
    15901 gtcggggtcc tggccctggc cacccgcgag ggtgctgagg tcaggcttcc ggcaggggcc
    15961 gggtgctgcg tgcgtgttca gttgtgctac ttgctgcgtg ctgtggaagc ccgcgagtcg
    16021 tgcttggccg gtcggccgcg atcagccctg gcgctgcttg tggcgcaggt tctcgcagat
    16081 gaccatgacc cgaccgttac ggcggatcac cttgcacttg tcgcagatct tcttgacgct
    16141 cggctggacc ttcattgcct cgtgtctttc agatcgggtg caacgggtcg gccaggcaca
    16201 ggcctgccca tgatccgttg caggggatcg ggagtgccat cggcggacgc cgagggcggg
    16261 tggtgcgaaa cagtcagcgg tagcggaaga cgatgcgccc gcgggacagg tcatacggcg
    16321 agagttccac cacgacccgg tcctcgggga ggatccggat gtagtgctgg cgcatcttgc
    16381 ccgagatgtg agcgagcact ttgtgaccgt tagagagctc caccctgaac gttgcgttcg
    16441 gtagtgcctc gacgatcgtg ccttcgatct cgatgacccc gtctttcttg gccatgtcct
    16501 ccgtcgttcc gttgtccgtg tgcccgggtc gaaggtgggc acgccacaat gacgcgttcc
    16561 acccgggagt cgcgagcaca cgacacccaa cggacgatcc tacggcaagc gcgcggcata
    16621 ccagaaatcg cgggtgcgtc cggcgccggg tgggccgagc cggtcaggcg ttcccgcggg
    16681 ctcgcttggg gcgcggggtg tccagcgcct cgtcgatgtg ctggcgccgc agcgtgatca
    16741 cccggtccag gacggcgtcg gggatcggct ggtcggtctg gaaccggatc gtgcccttgg
    16801 agaggctgaa cccgtcgagg tcggccgcca cgaggtcgac gatgacgccg ctgtgggggt
    16861 agagcgcgag gtgcgccttg gcctccaccg cagacagcag cggcttcccg cggtgcagca
    16921 ggacgggcat cccgtatgcc gtgccctcga ccagcccggg cacgaggtcg tgcgcgcggg
    16981 cgacgacgcg ctccagggcc gggcgtcgag cagcgtcgac ggtggccagg tagtcggtga
    17041 ccatgcccat atcgacgctc tcctagtcgc tcagcggggc gaacgcagca cccagggccg
    17101 ccaatcgggc cgcgccaccg tcaatcgcgg tgaggaccca caggccctcg tcacggacag
    17161 cgaccgtgtg ctcccagtgg gccgcccgcg agccgtcgga cgtgacgacg gtccagccgt
    17221 cggccagcac gtcgctctcg gcggaaccca gggtgatcat cggctcgatg gccccggtgt
    17281 agcccgagcg caccatgggg ccgcgggtcc gcacggcgta gttgaagatc tgcgggtcct
    17341 ggtgcatctc ggtcccgatg gtgtggccga cgtagtcctc gacgatgccg tacgtctgcc
    17401 cgttggccgc cgcggcggcc tcgatgcagg cctcgacggc ctcgccgacg gcatacagac
    17461 gctccccgac ccgcagcgcg gcgatgccgg catacagcga gtcctcggtc gcgcgggaga
    17521 gcgcgaggtc ctcggggcgg gccgcttcga cgccgccgac gatgacggtg acggccgcat
    17581 cgccgtgcca gccggcgacg atcgccccgc agtcgatgga cacgaggtca ccggcgcgca
    17641 gggcacgctc ccccgggatg ccgtggacga tctgctcgtt gacagacgtg cacagggtat
    17701 gccggtagcc cggcacctcg gggaaggacg gtcgcccacc gtggtcacgg atgtgggctt
    17761 cggcgagacg gtcgagttcg agggtcgtca cgccgtcagc gacagcagcc gcgaccacct
    17821 caagggtctg tccgaccaag aggccggcgg cccgcatgag cagcaactgg tcgtcggtgc
    17881 gcccgggcgt cttgcgacga gcagacaagc gatcagccct gcaggtcggc cagcgcggcg
    17941 acgatgcgcg cggtgatctc gtcgatgtcg cccatcccgt cgacctggcg caacttgccc
    18001 tggtcgccgt agatggcggt cagcggcgcg gtggccgccc ggtaaatgct catccgctcg
    18061 cggatgacct cttcggtgtc gtcggcccgg ccctggtcgg ccgcccgagc gagcaggcga
    18121 gccaccatgg cgtcctcatc gacgaccagc tcgagagctg cgtcgatctg gcggccggtc
    18181 tcttcgaggt gcgcgtcgag agtgtgcagc tgacccaccg tgcgggggta gccgtccagc
    18241 aggaagccgg gagcgcagtc ggcctcggcc aaccgatcgc ggaccagcgc gttggtcagc
    18301 tcgtcaggga cgtagccgcc ggtcgccatg atcgcctcga cctgcttgcc cagctcggtt
    18361 tggcccttga cgttggcgcg gaagatcgcg cccgtcgaga tggccgggat gccgaactgc
    18421 tcggcgatgc gctcggcctg agtgcccttg cctgccccgg gggggcccat gatgaccaac
    18481 ctcatcggag gaacccttcg tagttgcgct gctgcagctg agcctggatc tgcttcaccg
    18541 tctcgaggcc gacgcccaca atgatgagga tcgacgtgcc gccgaacggg aagttctggt
    18601 tcgcattgaa caacttcagt gcgatgagcg ggatcaggga gatcgcggcc aggtagatcg
    18661 ccccagggac cgtgatgcgc gtcaagacgt agtcgaggta ctccgcggtg ggccgaccgg
    18721 cccggatgcc ggggatgaag ccgccgtagc gcttcatgtt gtcggcaacc tcgaccgggt
    18781 tgaaggtgat cgacacgtag aagaacgtga agaagaggat caaggcgaca tagaccgcca
    18841 tgtagagggc gctgtccccc tggaccaggt tgctctggac gaagatcacc cagtcgggag
    18901 gtagcgagcc gtcctggggc gtcgcgaact gggccaccaa ggccggcagg gcaagcagcg
    18961 acgaggcgaa gatgaccggg atgacgccgg ccatgttgac cttgagcggg atgtaggtcg
    19021 agctgccgcc atacatccgg cggccgacct ggcgcttggc gtactgcacg gggatccggc
    19081 gttgggactg ctcgacgaag atgacgagcg ccatgacgaa gaagccgacg aggatgacgc
    19141 cgatgaagac gtcccagcgg ccttcctgct gctggatggc ccagagcgag gacgggaagc
    19201 ccgcagcgat ggacgtgaag atgagcagcg acatgccgtt gccgatgccg cggtcggtga
    19261 tcaactcacc cagccacatg atcaggccgg ttcctgcggt cagcgtgagg accatcatga
    19321 ggatggtcga gacgttggtg tcggtgagga tctcggtgca gttggggccg aagatctgcg
    19381 acgggttgcg cgcgaaggtg atgagggtgg ccgactgcag gatcgccaga ccgatggtca
    19441 ggtagcggct gtactgcgtc atcttggtct gccccgcctg gccctccttc ttcaacgcct
    19501 cgaagcgagg gatgacgacg gtgaggagct ggacgatgat cgtcgcggtg atgtagggca
    19561 tgatgcccag cgcgaagatc gacaactgca gcaacgcgcc accgctgaag aggttgacca
    19621 gaccgatgaa gccctgtgcc gggtcggccc cggccagaca ctgcgccacc ttggtgtagc
    19681 tcacgcccgg ggtcgggatc gccgacccca gccggaagat cgccatgatg agcagcgtga
    19741 acagcagctt cttgcgcagg tcgggcgtgc ggaacgcacg cgagactgct gtgagcacgg
    19801 ggtcctcctc ggtgcctcgc gcgggagcgc gataatcgtc ggtgcgttcg cttggcaaga
    19861 taacaccccg ggcgcggttt gccctggccc ggggtgtcac ctcacgtcgt ttgcgcgccg
    19921 tgggtgcgcg gtcggcatac ccaggtggta tgccgtgcgc ggccgcggtc aggcttgcga
    19981 aacactcccg ccggcggctt cgatcttgtc cttggcagac gtggagaacg cgtcggcggt
    20041 gatgttgacc ttgacggaaa tctcgccggt tccgaggacc ttgaccaact ggccgtcacg
    20101 gacggcaccc ttggcgacca ggtcggcaac cgtgatctcc ccgccttcgg ggtagagcgc
    20161 accgatccgg tcgaggttga cgacctggta gacggtgcgg aacgggttct tgaagccacg
    20221 cagcttgggc atgcgcatgt gcatgggcat ctgcccaccc tcgaaggcct cggacacctg
    20281 gtagcgggcc ttggtgccct tggtgccacg accggccgtc ttacccttgg agccctcgcc
    20341 acgacccacg cgggtcttcg gcgtcttggc acccggggcc gggcgcaggt ggtgaacctt
    20401 gagcgtcgtg cccgacgccg tgtcggacgt gctcacagtg ctcttggcca tggtcagtca
    20461 acctcctcga cggccacgag gtgcgtgacc gcgcgaacca tgccccgaat ctcgggacgg
    20521 tcctccttga cgacggtgtc gccgatgcgc ttgaggccca gcgagcgcag ggtctcgcgg
    20581 gcgaaccgct tctgtccgac gtgaccacga gtctgggtca ccttgagacg agccatcatg
    20641 caccgacctt gtctgccttg gcagcggccg acttggcggc ggcagcggcc tgggcacgca
    20701 gcatcgcggc gggggcgacg tcctcgacgg ccttgccacg gcgcgcggcg accgactcag
    20761 ggcgctcgag ctgacgcagc gcggccaccg tcgcgtggac gatgttgatc gcgttggacg
    20821 acccgaggct cttggagagc acgtcgtgga tgccggcgca ctcaagaacg gcgcgcaccg
    20881 gaccaccggc gataacacca gtaccgggag cagccggacg cagcaggacg acgcctgcag
    20941 cggcctcacc ctgcaccggg tgcgggatcg tgccctggtt gcggggaacc ttgaagaacg
    21001 ccttcttggc ctcctcgaca cccttcgcga tcgcggcggg cacctccttg gccttgccgt
    21061 agcccacgcc cacggtgccg tcaccgtcac cgacgacgac cagagccgtg aagctgaacc
    21121 ggcggccacc cttgacgacc ttggccaccc ggttgatcgt cacgacgcgc tcgagatact
    21181 ggctcttctc ctgctcgcgc gagtcacgac gaccatcacg gccgtcccga ccaccgcgac
    21241 cgcggcgctc gccgctctcg ccgccggcag cggtacccgc accggctccg gtgcctcgac
    21301 gctggggtcc gggcatcagt ggttcctcat ctcagtcagt ccgttcgggg tcacgatgct
    21361 cacagcgaga gccctccctc gcgcgcaccg tcggcgatcg ccgcgatccg gccgtggtac
    21421 ttgttgccac cacggtcgaa gacgacggcc tcgacaccgg ctgccttggc ccgctcggcg
    21481 atcagctcgc cgaccttctt ggccttggcg gtcttgtcgc cgtcgaaggc acgcaggtcg
    21541 ctctccatcg tcgaggcgca cgcgagggtc ttgcccgcgg tgtcgtcgac gatctgggcg
    21601 aacaggtgcc gggtcgagcg agacacgacc aggcgaggac gctcggggct tcccgagatc
    21661 ttcttgcggc cgcgcacctg gcggcggatc cgtgcggcgg ccttgctctt gccgcgcttg
    21721 atgatcattg ccatggctta cttcttcccg gcctttccga ccttgcgacg gatctgctca
    21781 cccgcgtatc gaatgccctt ggccttgtac gggtcgggct tgcgcagctt gcggatgttg
    21841 gcggcgacct cgccgacctg ctgcttgtcg atgccctgga ccgagaaacg ggtgggggcc
    21901 tcgacgacga agctgatgcc ggccggcggc tcgaccgtga tcgagtggct gtagccgagg
    21961 gcgaactcga ggttggcacc cttggcctgc acccggtaac cggtgccgac gatttccatc
    22021 ttcttctcgt aacccgcggt gacgccctgg accatgttgg cgatcagggt gcgggtgagc
    22081 ccgtgcaacg agcgcgacaa gcgctcgtcg tcgggacggg tgacctcgac agcaccgctc
    22141 tcgcccttgg cgacagcgat gggctcggca acaacgaggg ccaactgccc cttggggccc
    22201 ttgaccgtga cggcctggcc gtcaatggcg atgtcaaccc cggcaggaac gacgacaggg
    22261 aggcgtccaa ttcgcgacat gagtagctcc ccttaccaga cgtaggcgag gacttccccg
    22321 ccgacgccct tctcgtgagc ctgcttgtca gtcaacaagc cggtcgaggt ggaaatgatc
    22381 gccacgccga gtccgccgag cacgcgcggc aggttggtcg acttggcgta gacccgcaga
    22441 ccgggcttgc tcacccggcg gacgccggcg atagagcgct cgcggttggg gccgtacttg
    22501 agcgtgatgg tcagcgtctt gccaacctcg gcgtcgctca cggcccagct accgatgtaa
    22561 ccctcacgct tgaggatctc ggcaatgttg gccttgagct tggaatacgg catcgcgacg
    22621 ctgtcgtggt gtgccgagtt ggcattgcgc acacgcgtga gcatgtccgc aatcgggtcg
    22681 gtcatcgtca tcgggcttcg tgcccttcct caccgcggtt tcgtcccggc cggcatacag
    22741 ccggggcgac cttcggtgtc gagaatcggt ggaacttggg gtcttgctag ggggtggaac
    22801 agggaggtgc tccccggggc gcgaagcccc ggcgacgtcc taccaggagc tctttgtcac
    22861 gcccgggagc tcaccgcggt gtgccatctc gcgaaggcag atacggcaga ggccgaactt
    22921 gcggtagacc gagtgcggac gaccgcactt ctggcaccgg gtgtaggcac ggaccttgaa
    22981 cttcggggtc gcgttggctt tgttgaccag ggcggtcttg gccatgtcag ttctccttga
    23041 acgggaagcc gagggccttg agcaacgcgc gcccctcgtc gtcggtggtc gccgtggtga
    23101 cgacggtgat gtccatcccg cggacccggt cgatgcggtc ctggtcgatc tcgtggaaca
    23161 tcgactgc
//
LOCUS       DAOMZY010000013        23134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_1893_length_23134_cov_5.980025, whole genome shotgun sequence.
ACCESSION   DAOMZY010000013 DAOMZY010000000
VERSION     DAOMZY010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 23134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23134
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1893_length_23134_cov_5.980025"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..605
                     /locus_tag="PLK64_01680"
     CDS             93..605
                     /locus_tag="PLK64_01680"
                     /inference="COORDINATES: protein motif:HMM:NF022161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HPV78480.1"
                     /translation="MQITETLVYPAAIEVVFDMMTDEAFHSRVCEATHAMSYSATVSR
                     QSDGATVITHREMPTNSFPDFARSMVGQSIDIVETIVYGPPGPHGTRIGEMSISMGSA
                     PIHLKGDIRIIPADDGTEVIIEGNLKASIPFVGGKVESAAAPSVISGIHKEYGVGLAW
                     LAERGHTDQD"
     gene            complement(708..2336)
                     /gene="pruA"
                     /locus_tag="PLK64_01685"
     CDS             complement(708..2336)
                     /gene="pruA"
                     /locus_tag="PLK64_01685"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HPV78481.1"
                     /translation="MDALFRVPAPANEPVLGYAPGSSERATLEGELARIGGEQIDLPS
                     TIGGRKVMGGGRTGKVVQPHAHRKVLGTMKLVTPTEAQSAIDAAKAAAPMWRDMPFAD
                     RAAILLKAAELLSGPWRATLNAATMLGQSKTAPQAEIDSACELIDFWRINVHFAQQIL
                     AEQPPLNSKGVWNRSDYRSLEGFVYAITPFNFTAIGGNLPTAPALMGNTVVWKPARTQ
                     QLAAHYTMLILEAAGLPPGVINLVTSDGATLSNAVLADRDFAGIHFTGSTGVFHSMWE
                     QVGANIGRYKTYPRLVGETGGKDFVVAHPSADIDVLRTALVRGAFEFQGQKCSAASRA
                     YVPASIWKRLSKDLADEVNSLTQGDVTDFGNFMGAVIDGGAFADHKAAIDRAHATPGV
                     SVVAGGTYDDSEGFFVRPTVLKCDDPTDESLRTEYFGPILSVHVYPDRSYDRMLDQME
                     SFAPYALTGSIIAQDRAAIVAAAHKLRYAAGNFYINDKPTGAVVGQQPFGGGRASGTN
                     DKAGAAQNLMRWTSVRSIKETFVPPTDYRYPSMG"
     gene            2508..3524
                     /locus_tag="PLK64_01690"
     CDS             2508..3524
                     /locus_tag="PLK64_01690"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HPV78482.1"
                     /translation="MSAATTTSERVAVFGTGSWGTAFASVLAEAGCEVRMWGKFEDEV
                     ADIQRHHASVRYLPDLRLPDSLTATTDPAEALAGAGIGVLAVPAQTLRANLAEWGPLL
                     PPGIVLVSLMKGLEKGTSKRMTEVVGECLDLAPEQIVAVSGPNIAREIAAREPAATTV
                     ASASLATAHRVADACATPAFRPYVHDDVVGAELGGSVKNVIAVASGMTEGLGLGHSVT
                     AAVITRGLTEMRLLGEAMGAHPATFAGLAGVGDLVVTCLSPHSRNSTFGRNLGTGMTV
                     AEVVAVTKQTAEGVSSCGAILALARSHGVDMPIVANVTAVVQEGRTGPEFAQVLMDDM
                     FGGR"
     gene            3550..4338
                     /locus_tag="PLK64_01695"
     CDS             3550..4338
                     /locus_tag="PLK64_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HPV78483.1"
                     /translation="MSAADYPYFAPPPFGLAHRGGASHPANVGIENTLAAFATAVDLG
                     YRFLETDVHATADGRLVAFHDEHLDRVTDRAGAIAELPWSEVAAARIAGSQPIPSLDD
                     LLDAFPEARINIDIKAPGAVVPLWETICRHAAYDRVCVGSFGERRLHAFRRLAGARVA
                     TAAGQLGTAALRYAPGRLSTLAHSPGQVLQIPTTHEVRGRTFPLVTPGLIAAAHRFGL
                     QVHVWTIDDPDEMVRLFDLGVDAIVSDRIDVLRDVLAARGAWPT"
     gene            4335..5936
                     /locus_tag="PLK64_01700"
     CDS             4335..5936
                     /locus_tag="PLK64_01700"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV78484.1"
                     /translation="MNRAARTGTAVLPRTDRAITAALLLLTTMIAFENMAVATAMPVV
                     AAELGALGSYGLAFSSMMTAMLLGIVLAGPWTDRAGPLPPVFVGQALFAIGAVLCGAA
                     PSFGILLLGRVITGLGAGLVVVVEFVVIARSFAPEQRPRVFSWLSAAWILPSLIGGPI
                     AGWLATEFNWRWVFWVIVLPALVAGGLLLQRRETLRSPGSYSAAQPTASASASASASD
                     ARRLADAAVAMGHVGALDDGALDATADAPVADPAVREAADPADDPADERRQHRRTARL
                     GAVVAGAAGVVQLAIHERPPAVSFLTAAAVLALIAVAIAAPKLLPPGTFRSARGLPSV
                     VLTRALLNGSFLGAFAYLPLMLTQEKGLDATSAGLVLAAGSLGWSAGSWAQGRSRRSG
                     IDERTGLVVLGASLLAVGWAAFVGLTLGHAPVWTFAAAVAVGGLGMGLGSTTLSGLVL
                     ELSPDHEHGRASAALQLADVLGTVVGIAAATAAFGIWHTTGAHRGFVIVWIGLAALGA
                     LGIVSAARCASETSRSGDDVPHRSL"
     gene            complement(5898..6635)
                     /locus_tag="PLK64_01705"
     CDS             complement(5898..6635)
                     /locus_tag="PLK64_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78485.1"
                     /translation="MTDLVFPDQQGYADLATLVRRARQADPDAAVRLQVTGAVLRVWV
                     GVLPGTGLLADGAAVGVRGIELANEDAGGSASEAGALDLVCASAALSDRFAYDGVGAS
                     RLSVPPASTYAAWAGALPAVAGWSSIGAISAQALIDAARAGIAEIAEGVDARQTGAAA
                     GSRAVQELRRRVWARAVPIPAGVDDGPVNGADDQRPTGFEGVPAGAAFAAYVLGFLPE
                     PSATVRVWRSGRWVRLQTAVGHVVARP"
     gene            complement(6643..7182)
                     /locus_tag="PLK64_01710"
     CDS             complement(6643..7182)
                     /locus_tag="PLK64_01710"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HPV78486.1"
                     /translation="MPDPILASTLASTLASTLASAPAATPAAATVAPDPTEVLVPLRW
                     SDMDAYGHVNNTQFLRLLEDARVAALPRWFPEGLALLERGILVVRHEIEYRAQLDYRP
                     EPVAIDLWVTRIGGAGYDLAYVVRDPDGVGSGTVYAVAETSLALFDFAANTPRRATAV
                     ERECLTAHLGAPAPLRKRR"
     gene            7223..8173
                     /locus_tag="PLK64_01715"
     CDS             7223..8173
                     /locus_tag="PLK64_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HPV78487.1"
                     /translation="MRLPDSAADSVADLLDVLDLTCLGEARIVVEGAPGDEETDLGES
                     TPIVFVGRSQKQAHGRVFGGQVLAQSLVAAARTVEPVDGVARPIHSLHAYFVRGGDDN
                     HPIRFVVERVRDGASFSTRRVQAVQYGQTILSMGCSFQEPADGMDHQDPMPPAPDPDD
                     IPTLAEALGEHLDPSAAQFFAARRPIELRHVEGNIYVKPGRQRAARQSVWLKARAELP
                     DDPVIHAAVLAYASDYPMLEPVLRRHGVAWQDPRLRPASLDHAMWFHRAPKADDWVLY
                     TMSSPSASGGRGLGVGRMFSADGALMASVAQEGMLRLKEG"
     gene            8236..9258
                     /locus_tag="PLK64_01720"
     CDS             8236..9258
                     /locus_tag="PLK64_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPV78488.1"
                     /translation="MTDTPAAYAVSDLLDILDLREVGTVHTGWAVASPSESPTAAVTA
                     DIATATATATAAVADLAVEVGAGSRVARVFAGPSQKQRYQRTFGGQLLAQAIIAAGRT
                     VAADPAGAGRPLHSTHTTFLVSGADADEVHYVVEPLSDGRSFSTRRVSALQHGTLLAI
                     VTASFQEASPGLDHAEPMPAAPSAESLPNVAEALAGVPEPYAVVCILDGPIELRHVEG
                     HLYAGARPDHVARQGVWLRSRRPMPDDPIIHAAFLAYASDYSILESVLRRHGTAWSDP
                     RLRQASLDHAMWFHRPGRADEWVLHAGHSPSASGGRGLGLGRMYAADGTLLASIAQEG
                     MLRLKG"
     gene            9374..10132
                     /gene="fabG"
                     /locus_tag="PLK64_01725"
     CDS             9374..10132
                     /gene="fabG"
                     /locus_tag="PLK64_01725"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HPV78489.1"
                     /translation="MSTTPRTAIVTGAARGIGAATAKRLAADGHAVAVLDLDEAACAA
                     TVSEIVAAGGRALAVGVNVADSASVAAGVERVAAELGGPTILVNNAGITRDNLLFKMT
                     EEDWDSVLSVHLRGAFLMSKACQKYMTEATFGRIVNLSSTSAQGNRGQANYAAAKAGM
                     QGFTKTLAIELGKFGVTCNAIAPGFIQTEMTKATADRLGVSFEDFIAFSAKEIPVARV
                     GQPEDIAATVSFLTSDEAGFVNGQVIYVAGGPKD"
     gene            complement(10228..11559)
                     /locus_tag="PLK64_01730"
     CDS             complement(10228..11559)
                     /locus_tag="PLK64_01730"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HPV78490.1"
                     /translation="MSSTSATVSGGASVADRRAAFVAARSALDGLTGLLCQVAGAELG
                     LLVGELDALASRAAGAVVAVVAEADSRGEISASQAGTSTQWVARHAPSLAAEGAGQVA
                     RLVQECRLPELATVRAAVEGGTVSVGVGLGVVSEFQALRPMLRNEARPTVLDGLVQMG
                     AEQGRRGVRRLRPALLAKFGDPGVFQEVQDAAGRLVALDRAVGDVLGAFTYRLVVDAQ
                     GKAALEAAIGPLSAPVPGPDGARDPRPPERRRGEALIEVCRRATAAGGIPPSGVKSTL
                     MVTMPFADLSNRLGAGEVLGSSEAGELIAPETVRKLACDAGVIPVVLGGEGEVLDLGR
                     ATRLFTAGQTAALWLRDRVCSFPECSIPAHWCDAHHLIHWLDGGVSQLSNAALLCGRH
                     HTVVHRDRLFGQVTSDGVDWDRRLGSYDRALAAPGHGGKAGEAAGPDPPAA"
     gene            11749..12318
                     /locus_tag="PLK64_01735"
     CDS             11749..12318
                     /locus_tag="PLK64_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78491.1"
                     /translation="MILPKVRDPRLVTIRRGGLLTDPDHHLLALWAAACAEHVLDLFE
                     AECPSDLRPRHAIAGARAWAAGELAMMQSRAAGGHAMGAARPLSGAARFAAYAAGQAA
                     CIPHVPEHDLGAAAYAIKAAQAAAAAGDDGEAAARRECQWQLDQLPDPIRALVLDDQA
                     RRNPICWSVFTEPGPLAAGPTHPSGGLQR"
     gene            complement(12632..13441)
                     /gene="ppk2"
                     /locus_tag="PLK64_01740"
                     /pseudo
     CDS             complement(12632..13441)
                     /gene="ppk2"
                     /locus_tag="PLK64_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006213342.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
     gene            13632..13889
                     /locus_tag="PLK64_01745"
     CDS             13632..13889
                     /locus_tag="PLK64_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097573 - glutathione oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycoredoxin"
                     /protein_id="HPV78492.1"
                     /translation="MSEFTPAPGTVTMFTTTWCGYCRRLKSQMEREGIGFTEVDIEVR
                     PEAADVVMSVNGGNQTVPTLIFPDGSAATNPSIGQVKARLA"
     gene            complement(13886..14905)
                     /gene="nudC"
                     /locus_tag="PLK64_01750"
     CDS             complement(13886..14905)
                     /gene="nudC"
                     /locus_tag="PLK64_01750"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HPV78493.1"
                     /translation="MEQTPGLRGLLLSHGGPDRAAHLREDADLLDRLLADAGTRVVAV
                     RGDRVLVAPVPAHSMAPTADPSPAPADGVAGALGLVLRSPSAADGGLLAVFLGLHPGE
                     DGERTAYLGVVEAPTDGDGDAGWQTLRAAGALLPDLDASLAATLLALANWHRSHDRCS
                     RCGAPSEPASAGWVRRCTRDGSQHFPRTDPSVIMSVIDDDGRLLLGRGATWPANQFSV
                     LAGFVEPGESLEAAVAREVLEESGVVVTEVSYLGSQPWPFPSSLMLGFTARAVTTALT
                     HDPTEMAEVRWVTRAEYAALLRSGAIRVPGSISIARRIIERWLGADLEVAAGHAVIEG
                     WRPTT"
     gene            14969..>16262
                     /locus_tag="PLK64_01755"
     CDS             14969..>16262
                     /locus_tag="PLK64_01755"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPV78494.1"
                     /translation="MSPESRSPAFLAALASAAVPGLDPVSARAVAPGPAGEFDAAIVI
                     DEQDRPWLVRCPRTPAAGARLESAAALAALLARRLPFQVPVPKGFAALPDGRAGVYPV
                     LPGRPLAFDALPAGPGLAADLGRAIAAIHNVDRGVFDEAGMPSYDADSYRTRHLADLD
                     RAAATGHVPTALLSRWEGALDDVTLWRFAPAPVHGGLTGSQVLAGFDSDEDAATGRVR
                     AVVGWEDAKVADPADDFAAIVTLAGPAAVESVLEAYANSRIERPDPHLRRRARLAAEF
                     AQLTNLLTAVTLGDPDRVRAMARRLVELERRVTDADLKAPEPVSVVPREVIVPKTVAA
                     ASPAAANGSGSGEDTVAVDPDTLFDDTAPLHGTAAHDDSVTHDDSVTLDGTVTLDDAA
                     ALDDTVAMDPRTLFDATEEIPTAHGPASPSRPDAPATPD"
     assembly_gap    16263..16362
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16387..19884)
                     /locus_tag="PLK64_01760"
     CDS             complement(16387..19884)
                     /locus_tag="PLK64_01760"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HPV78495.1"
                     /translation="MASVRHSAAEVARAAGLAHAPTPEQTAVIEAPLRPMLVVAGAGS
                     GKTETMAARVVWLVANGLVAPDQVLGLTFTRKAAAELAERIDQRLRRLQAAGLWTPAP
                     DEEGAEVLGGTPTVATYHSYAGRLVREHALRLGYEPDSRLLSEAAAWQYAAEVVSRYD
                     GDMSEMGKAESTAIGAVVDLAGELAEHLVDTGTLREVLDRIVRAIDATPLAGTAKTLP
                     ADLATLRGTLRARRALLPVVDAFGSLKRSRDAVDFADQVALAARLAVGFPDIGAGERA
                     RFRVVLLDEFQDTSEAQLVLLRSLFAVGDSGVPVTAVGDPNQSIYGWRGASATTLTRF
                     PAEFTDGSPTPVRQLATTWRNDASILKVANHLAAPLRASARVDVAPLAVRPDAGVGDV
                     VVARVGTHVEEAAYLADWLAGATSRAGTTAAVLCRKRSQFGPVMEALDAAGVPYEVVG
                     LGGLLLTPEILDLVSALRAIADPSRGDALMRLLTGPMCRLGAADLDGLHEWARFRQKV
                     VKAESQGRLAIDLDPGHRDEDDGVEGPADALVDLAPEVLDEPSIVEALDDLPPEVWRG
                     TEGQYVGAGALVRLKGLSTALRRLRRLGALPLPDLVGEAERALGLDVEVLSRPGYTPD
                     TSRAHLDAFADVAADFASSADRPTLIGFLAWLDAAVAQERGLERATLEPSAEAVQILT
                     IHAAKGLEWDVVAVPGLVEGTFPAREGSPTPKHNGARWVVPDPTPGGWLVGLAGVPFA
                     LRGDRDGLPTLQVEGASDSKALSERVAAFRVEEGLRDLDEERRLAYVAVTRARRRLLL
                     TASVWTNTQRHKVTSRFLREVVRARDELGVRVAHWDAMPDPAAVGTNPVADETVRVLW
                     PTPPDQSRLDDAQRAAAVVAMARRERAAAGAAHAGTSDVGTIGGGSIGAGAIGAGALG
                     AGAIGAATGEGVAAYPRAALLAPRGGGADDPYAADVDLLLAERALARETGPQVVEVPR
                     HLSASDIVRLAEDPERFAGERRRPMPAEPALAARRGTAFHAWVEQHYARATLLDSAEL
                     PGAADDDPGSDAELPQLKEAFLASEWAWRTPIDVEVAIETVIDGIAVRGRIDAVFGRA
                     DGGVTVVDWKTGPQPSGADAAHRALQVGAYALAYARLRGLLPDQVDAAFYYARTGTTV
                     RPTLPDEAELIRLLGSIAD"
     gene            complement(19884..23033)
                     /locus_tag="PLK64_01765"
     CDS             complement(19884..23033)
                     /locus_tag="PLK64_01765"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HPV78496.1"
                     /translation="MLTLRRAPALVVEAPTLDAQQQAALALDAPVARVLGGPGTGTST
                     VAVEVVLDRVDRLGLTPDQCLVLAPTRLAAAALRDRLTARLARTSTEPLARTHQAFGF
                     AVLRREAALRGAPASRLLSGPEQDVVLRDLLAGHVASGGGPQWPERVAAALGTRGFRA
                     ELRDLLMRAVEHGVDAANLAELGRQHDRPEWVSAAAVLDEYDEVTALSQPGAYDPAWI
                     LTAAADLLEDDPEALERVREQIRLIVVDDAQELTWAAARLLRTIAHPGMQIILIGDPD
                     SAVQTFRGADPRLLWWDRWPALRDAPTVVLRTAYRTPTRLHDAASEVVRRIGATGGAD
                     RRGSQPVRDGGEVQVALLRSVAQEAGFIASTLRRAHLQEGMPWRDMAVLVRGRGRAGT
                     LRRVLSAAGVPLAPPDGEVPVRDEVAVRPLLALLDLCLRLARDPDAMIAPTEAVDLCL
                     SVLGGGDAVSLRRLRRALRRIELDGGGRRSSDELLAAALGDPVLSAQAGVDGEPLRRL
                     TRAILAGVGAARRTDDAAGWAPEVSPESVLWAIWSALGVAASWRRAALDGGSRGGRAD
                     RDLDAVVGLFDAAARYADRLPGSGPDAFLDHVLGEEVPGDTLALRAPAVDTVAVLTPA
                     AAAGREWRLVCVSGVQEGVWPDLRLRGSLLGSEHLVDVVTGRGGSVRAAQAAVRYDET
                     RLFHVAVTRASERLIVSAVRNDDEQPSVYLDLLDPLPADADGALGRPFTPFGRALTTA
                     GVVGALRQVAASVTDEVAAARAGRQLARLADDGVTGADPGQWWSLVPAADDRPRRGPD
                     QAVRVSPSKVEQFAKCELAWLLRASGGDGVKSASASIGTLVHDVVAELGDVDAPTLQA
                     EIERRWAQLGLTPGWVQERQRAEAHAMAGRAAAYFVSPAAAPWERVGVEVDALVEVGR
                     AVLKGRVDRLERHTDGSLRVIDYKTGSSKPTAAELARHPQLGVYQAAVEHGAFGDLGT
                     RSAGAALLQLGKASNKSVTLQGQPALADDDDPGWASRLVEETAAGMGGGQFAATPGEH
                     CRMCPVVAACPARPEGQSI"
BASE COUNT         3400 a         8402 c         7919 g         3313 t
ORIGIN      
        1 gctcggggcc gggcccaact cgcccgccac ctggcccagg ccagccagca ggtggcgcgc
       61 gcatccgcgg cggctcgcta aggtcgggaa ccatgcagat caccgagacc ctcgtctacc
      121 ccgccgccat cgaggtcgtc ttcgacatga tgaccgacga ggcgttccac tcccgggtgt
      181 gcgaggcgac ccacgccatg agctactccg cgacggtgag ccgccaaagt gatggggcga
      241 cggtcatcac ccaccgtgag atgccgacga acagcttccc cgacttcgct cgctcgatgg
      301 tcgggcagag catcgacatc gtcgagacga tcgtctacgg cccgccggga ccacacggga
      361 cgcgcatcgg cgagatgtcg atctcgatgg gctcagcccc gatccatctc aagggtgaca
      421 tccgaatcat ccccgccgat gacggcaccg aagtgatcat cgagggcaac ctcaaggcga
      481 gcatcccgtt cgtcggcggc aaggtcgagt ccgccgccgc gccgtcggtg atctccggga
      541 tccataagga gtacggcgtc ggcctggcct ggctcgccga acgcggtcac accgaccagg
      601 actgactccc ccccgcgcgc aggaccctcc aggtatgccg cgcgcctagg tgagcgggcg
      661 gcatacctga tggtggtggt cagcccacgg cggggaagcc gcgtcgatca acccatcgag
      721 gggtagcggt agtccgtcgg cgggacgaac gtctccttga tcgaacggac cgaggtccag
      781 cgcatgaggt tctgtgcggc accggccttg tcgttggtgc cggacgcacg gccgccgccg
      841 aagggctgct ggccgacgac cgctccggtg ggcttgtcgt tgatgtagaa gttgcctgcg
      901 gcataccgga gcttgtgggc ggctgcgacg atcgctgcgc ggtcctgggc gatgatcgac
      961 ccggtgaggg cgtagggcgc gaaggactcc atctggtcga gcatcctgtc gtaggaccga
     1021 tcggggtaga cgtgcaccga gaggatcggg ccgaagtact ccgtgcgcag ggactcgtcg
     1081 gtcgggtcgt cacacttgag gaccgtgggc cgcacgaaga agccctcgga gtcgtcatag
     1141 gtgccaccgg cgacgactga cacgccggga gtcgcgtgag cgcggtcgat ggcggccttg
     1201 tggtcggcga acgcgccgcc gtcgatgacc gcccccatga agttgccgaa gtcggtgacg
     1261 tcgccctggg tgagcgagtt gacctcgtcg gccaggtcct tgctcaggcg cttccaaatg
     1321 gacgccggga cataggcgcg cgaggcggcc gaacacttct ggccctggaa ctcgaaggct
     1381 ccacggacga gcgcggtgcg cagcacgtcg atgtccgccg acgggtgcgc gacgacgaag
     1441 tccttaccgc cggtctcacc gaccaggcgc gggtaggtct tgtagcggcc gatattggcc
     1501 ccgacctgct cccacatcga gtggaagacg ccggtcgacc cggtgaagtg gatgccggcg
     1561 aagtcgcggt cggcgaggac cgcgttggac agggtcgcgc catcggaggt gaccaggttg
     1621 atgacgccgg gaggcagccc cgcggcttcg aggatgagca tcgtgtagtg ggcggccagt
     1681 tgctgcgtgc gggccggctt ccagacgacg gtgtttccca tgagtgcggg cgcggtcggc
     1741 aggttgccgc cgatcgccgt gaagttgaac ggcgtgatcg cgtagacgaa gccttcgagc
     1801 gatcggtagt cgctgcggtt ccacacaccc ttggagttga gcggcggctg ctcggcaagg
     1861 atctgctggg cgaagtggac gttgatccgc cagaagtcga tgagctcgca ggccgagtcg
     1921 atctcagcct gcggggcggt cttgctctgc ccgagcatcg tcgccgcatt gagcgttgcc
     1981 cgccacggcc cggagagcag ctcggcagcc ttgagcagga tggcggcccg gtcggcgaac
     2041 ggcatatcgc gccacatcgg ggcggcggcc ttggcggcgt cgatcgcgct ctgggcctcc
     2101 gtcggggtga cgagcttcat cgtcccgaga accttgcggt gggcgtgcgg ctgaacgacc
     2161 ttgccggtcc ggccgccccc catgaccttg cggccaccga tcgtgctcgg caggtcgatc
     2221 tgctcgccgc cgatccgggc gagttcgcct tcgagcgtcg cgcgctcgga gctacctggc
     2281 gcatatccca ggaccggctc gttggcgggg gccggcacac ggaacagggc atccatcgga
     2341 gggttcctcc tcgtcgaggg tcacgtcatc ggccgagcgg gtggcgtcgg cctcgtccgc
     2401 tcatggtgtc acgttcccgc gggtgcctgt cagcagtcgg tcgttcgtgc gccttccgat
     2461 tcacgtccct ctcgcggtcg gcgcggtcgc catgccacgc ttgcgccgtg agtgcggcga
     2521 cgacgacgtc agagcgagta gcggtcttcg ggaccggtag ttgggggacg gcgttcgcgt
     2581 ccgtgctggc tgaggccggg tgcgaggtgc ggatgtgggg gaagttcgag gacgaggtcg
     2641 cggacatcca gcgccatcac gcgagcgtgc gctacctgcc cgacctgcgc ctgcccgaca
     2701 gcctgacggc cacgaccgac cccgccgaag ctctggctgg cgccggcatc ggcgtgctgg
     2761 ccgtgccggc ccagaccttg cgggccaacc tcgccgagtg ggggccgttg ctgccgccgg
     2821 ggatcgtcct ggtgtcgctg atgaaaggcc tggagaaggg cacgtccaag cggatgaccg
     2881 aggtcgtcgg cgagtgcctc gacctcgcgc ccgagcagat cgttgccgtg agcggcccca
     2941 acatcgccag ggagatcgcc gcacgcgagc cggccgcgac gacggtcgcg agcgcctcgc
     3001 tcgccaccgc ccaccgggtc gccgacgcct gcgccacccc ggcgttccgg ccgtacgtcc
     3061 atgacgacgt cgtcggggcc gagctcggcg gaagcgtcaa gaacgtcatc gcggtggcct
     3121 cggggatgac ggaaggcctg ggcctcggtc actccgtcac cgctgccgtc atcacgcgcg
     3181 ggctgaccga gatgcgattg ctcggcgaag ccatgggagc gcacccggcg accttcgccg
     3241 gtttggccgg ggtcggagac ctggtcgtca cctgtttgtc gccgcattcg cgcaactcga
     3301 cgttcggccg gaacctcggc accggcatga cggtggcgga ggtcgtcgcc gtcacgaagc
     3361 agaccgcgga aggggtctct tcctgcgggg cgatcctcgc actcgcgcgg tcacacgggg
     3421 tcgacatgcc gatcgtcgcc aacgtcaccg ccgtcgtcca ggaaggccgc accgggcccg
     3481 agttcgccca ggtgctcatg gacgacatgt tcggcggccg gtagccccgc ccgtggcagg
     3541 gtgttcctcg tgagcgcggc cgactacccc tacttcgcgc ctccgccgtt cgggctggcc
     3601 caccgaggcg gggcgagtca ccctgccaac gtcggaatcg agaacaccct ggccgcgttc
     3661 gccacggcag tcgacctggg ctatcgcttc ctcgagaccg acgtccacgc caccgccgac
     3721 gggcggttgg tggccttcca tgacgagcat ctcgaccgcg tgaccgaccg ggcgggcgcc
     3781 atcgccgagt tgccgtggag cgaggtcgcg gccgcccgga tcgctggcag ccaaccgatc
     3841 cccagcctcg acgacctgct cgacgccttc cccgaggcgc ggatcaacat cgacatcaag
     3901 gcccccggcg ccgtggtgcc gttgtgggag acgatctgca ggcacgcggc atacgaccgg
     3961 gtgtgtgtgg ggtcgttcgg ggaacgtcgc ctgcacgcgt tccgacgcct ggccggagcc
     4021 cgcgtggcca ccgctgccgg ccaactcggc acggctgccc tgagatatgc gcccggccgg
     4081 ctgtccacgc tggcgcactc gcccggacag gtcctgcaga tccccacgac gcacgaggtg
     4141 cgcgggcgga cgttcccgtt ggtgacgcca gggcttattg cggcggcgca ccggtttggg
     4201 ctgcaggtgc atgtctggac catcgacgac cccgacgaaa tggtccggtt gttcgacctg
     4261 ggtgtcgacg ccatcgtcag tgaccggatc gacgtgctgc gggacgtgct cgccgcccga
     4321 ggggcgtggc cgacgtgaac cgcgccgccc gcaccggcac tgccgtcctc ccccgcacgg
     4381 atcgggccat cacggctgcc ttgctgctgc tgacgacgat gatcgccttc gagaacatgg
     4441 ccgtcgccac ggccatgccg gtcgtggccg ccgagttggg agcgctcggg tcctacggcc
     4501 tggccttctc gtcgatgatg accgcgatgc tcttgggcat cgtcctggct ggcccgtgga
     4561 ccgaccgcgc cggcccgctg ccgccggtgt tcgtcggcca ggcgctgttc gccatcggag
     4621 ccgttctctg tggcgctgcc ccctcgttcg gcatcctgct gctcgggcgg gtcatcacgg
     4681 ggctcggtgc cgggttggtc gtcgtcgtgg agtttgtcgt catcgcacgg tcgttcgcgc
     4741 ccgagcagcg acctcgggtg ttctcctggc tgtccgcggc gtggatcctt ccgtcgctca
     4801 tcggtggacc gatcgccggc tggttggcga cggagttcaa ctggcgctgg gtcttctggg
     4861 tgatcgtcct tcccgcgctg gtggcagggg ggctgttgct ccaacgtcgc gagacgttgc
     4921 gctcccccgg cagttactcg gccgcccagc cgaccgcgag cgcgagcgcg agcgcgagcg
     4981 cgagcgatgc tcgacgactg gcggatgcag cggtcgcgat gggccacgtc ggcgcactcg
     5041 acgacggcgc attggacgcc acggccgacg ccccagttgc cgacccagcc gtccgcgaag
     5101 ccgccgaccc tgccgatgac cctgccgacg agcgacgcca acatcgacgc acggcccgtc
     5161 tgggcgccgt cgtggccggc gcggccggcg tggtgcagtt ggcgatccac gaacggcccc
     5221 ctgccgtgtc gttcctgact gcggccgccg tcctagctct gatcgccgtc gccatcgctg
     5281 cgcccaagtt gctgcctccc gggacgttcc gcagcgcccg cggcctgccc tcggtggtcc
     5341 tcaccagggc attgctcaac gggtcgttcc tcggcgcctt cgcctacctg ccgctcatgc
     5401 tcacccagga gaaggggctt gacgccacct cggccgggct cgttcttgcg gccggctcgc
     5461 tcggctggag tgccgggtcc tgggcgcagg gtcgctcacg acggtccggc atcgacgagc
     5521 ggaccggctt ggtcgtcttg ggcgcatccc tgcttgcggt cgggtgggcc gcgttcgtcg
     5581 gcctgaccct gggacacgca ccggtgtgga cttttgcggc ggccgtcgct gtcggcgggt
     5641 tgggcatggg cctggggtca acgacgctgt cggggctggt ccttgagctg agccccgacc
     5701 acgaacacgg gcgggcgtcg gccgcgttgc aactagccga cgtactcggg accgtagtcg
     5761 gcattgctgc cgcgacggcg gcgttcggga tctggcacac gacaggtgcc caccgcggct
     5821 tcgtcatcgt gtggattggg ctggctgcct tgggcgcgct ggggatcgtc tcggccgccc
     5881 gctgcgcatc ggagacctca cggtcgggcg acgacgtgcc ccaccgcagt ctgtagccga
     5941 acccagcgcc cgcttcgcca tacccgcacc gtcgcgctcg gctccggaag gaacccgagc
     6001 acgtatgccg cgaatgccgc ccctgcgggg acgccctcaa acccggttgg ccgctggtca
     6061 tcggcaccgt tgacgggccc gtcgtcgacc cctgcgggga tcgggacggc ccgcgcccag
     6121 acccggcggc gcaactcctg gaccgcccga gacccggcgg cggcacccgt ctgtcgcgca
     6181 tcgacgcctt cggcgatctc ggcgatgccg gcccgggccg cgtcgatgag tgcctgcgcg
     6241 gagatggctc caatcgacga ccaccccgcg accgccggga gggcgccggc ccacgcggca
     6301 tacgtgctgg ccggagggac cgacagcctg ctcgcgccga caccgtcgta ggcgaaccgg
     6361 tcgctcagcg cggccgaggc gcagacgagg tcgagcgcgc cggcctctga cgcggaaccg
     6421 cccgcatcct cattcgccaa ctcgatgccc cggacgccga cagcggctcc gtcggcgagc
     6481 agtcccgtgc ccggaaggac ccccacccac accctgagga ccgcaccggt gacctgcagg
     6541 cggacggccg cgtcgggatc ggcctggcgg gcccgacgca cgagagtggc cagatcggca
     6601 tacccctgct gatcggggaa gacgagatcg gtcatcgcgt gctcatctgc gcttacgcag
     6661 cggcgcgggg gcaccgaggt gggcggtgag gcactcgcgc tccacggcag tcgcccggcg
     6721 cggtgtgttg gcggcgaagt cgaacaaggc aagggacgtc tcggctacgg cgtagaccgt
     6781 gccggagccc accccgtcgg ggtcacggac gacgtaggcc aggtcgtagc ctgctccccc
     6841 gatgcgggtc acccagaggt cgatggcgac cggctccggg cggtagtcca gttgcgcgcg
     6901 gtactcgatc tcgtgacgaa cgacgagaat gccccgttcg agcagcgcca ggccctccgg
     6961 gaaccatcgg ggcagggctg ccacgcgggc gtcttcgagc agccggagga actgcgtgtt
     7021 gttgacatgg ccgtaggcgt ccatgtccga ccaccgcaac gggacgagca cctcggtcgg
     7081 atcgggtgcc acagtggcgg cggccggtgt ggcggccggc gcgctggcca acgtgctggc
     7141 caacgtgctg gccaacgtgc tggccaagat ggggtcgggc atgggcgcca tcctgtcagt
     7201 ccccagggat agcctcacgc tcatgagact gcccgactcg gccgccgact ctgttgctga
     7261 cctgctcgac gttcttgacc tcacctgctt gggcgaggca cggatcgtcg tcgagggcgc
     7321 gcccggtgac gaggagaccg atctgggcga gtcgaccccg atcgtcttcg tcgggcgcag
     7381 ccagaaacag gcgcatgggc gggtgttcgg aggccaggtg ctggctcagt ccctcgtcgc
     7441 ggcggcccgg acggttgagc cggtcgacgg cgtagcccgc ccgatccact cgctgcacgc
     7501 ctatttcgtg cgcggcggcg acgacaacca tccgatccgg ttcgtcgtcg aacgagtgcg
     7561 ggacggggcg tcgttctcga cgcggcgggt ccaagcggtg cagtacggcc agaccatcct
     7621 gtcgatgggg tgctcgttcc aagagccggc ggacggcatg gaccatcagg atccgatgcc
     7681 gcccgcgcct gaccctgacg acatccccac gctggccgag gccctgggtg agcacctcga
     7741 cccgtcggcc gcgcagttct tcgccgctcg ccgacccatc gagttgcggc acgtcgaggg
     7801 caacatctat gtcaagcccg ggcggcagcg agcggcgcgc caaagcgtgt ggctcaaggc
     7861 ccgcgccgaa ctgcccgacg acccggtgat ccacgcagcg gtgctcgcct acgcctccga
     7921 ctaccccatg ctcgaaccag tgctgcgccg ccatggggtc gcctggcagg acccacgcct
     7981 gcgcccggcc agcctcgatc acgcgatgtg gttccaccgc gcgcccaagg ccgatgactg
     8041 ggtgctctac acgatgtcca gcccatcggc ctctggtggt cggggtttgg gagtcgggcg
     8101 gatgttcagc gccgacgggg cactcatggc atcggtggcc caggaaggca tgctccgtct
     8161 caaggagggg tgagcagccc cgtgcgtagt ggtgacccgt gcgcagtggt gagcgggcgg
     8221 cataccctcg gggtcgtgac tgacaccccg gctgcgtatg ccgtcagcga cctcctcgac
     8281 atcctcgacc ttcgcgaggt gggcaccgtg cacacggggt gggcggtcgc ctccccgtcc
     8341 gagagtccca cggcagccgt tacagccgac atcgccacgg ccacggccac ggcgaccgcc
     8401 gctgtcgctg acctcgccgt cgaggtgggc gccgggtcgc gggtggcgcg ggtgttcgcg
     8461 ggaccgagcc agaagcagcg ctaccagcgg acgttcggcg ggcagttgct cgcccaggcg
     8521 atcatcgccg ccgggcgcac ggtcgccgcc gacccggccg gtgccgggcg acctctgcac
     8581 tcgacccaca cgaccttcct ggtctcgggg gccgacgccg acgaggtcca ctacgttgtc
     8641 gagccgctct cggacggccg gtccttctcg acccggcggg tgtcggcgct acagcacggc
     8701 acgctgttgg cgatcgtgac cgcatcgttc caggaggcgt cgccggggtt ggaccacgcc
     8761 gagccgatgc ccgcggcgcc gagcgccgag agcctgccca acgttgccga ggctctggcc
     8821 ggtgtccccg agccgtatgc cgtggtctgc atcctcgacg gccccatcga gctgcggcac
     8881 gtcgaagggc acctttacgc gggggcgcgg ccggaccacg tcgcgcgaca gggcgtctgg
     8941 ctgcgttcgc ggcgcccgat gcccgacgat ccgatcatcc acgcggcgtt cctcgcctac
     9001 gcctcggact attcgatcct cgaatcggtg ctgcgccgcc atgggacggc gtggtccgat
     9061 ccgcgactgc gccaagcgag cctcgaccac gccatgtggt tccaccggcc gggccgcgcg
     9121 gacgaatggg ttctacatgc gggccactct ccgtcggcct ccggcgggcg cgggctcggc
     9181 ctgggccgga tgtatgccgc ggacggcacc ttgctcgcct cgatcgccca ggaagggatg
     9241 ctgcggctca aggggtgatg caccccaccc tcagcgaggc agagacggcc tgcccgcggc
     9301 ataccgtgcc gtcgtaggct cgaagcgaca cgcgccggcc gtgcgcgcga cccgtccact
     9361 cgaggagtct cccgtgagca ccaccccccg caccgccatc gtcaccggag cagcccgcgg
     9421 aatcggcgcg gccaccgcca agcggctcgc cgccgacgga cacgcggttg cagtgcttga
     9481 ccttgacgaa gctgcttgcg ctgcaacggt ttccgagatc gtcgccgcag gcggtcgcgc
     9541 tctggcagtg ggcgtcaacg tcgccgacag cgcgtcggtc gctgctggtg tcgagcgggt
     9601 tgccgccgag ctcggaggcc cgacgatcct cgtgaacaac gcggggatca cccgcgacaa
     9661 cctgctgttc aagatgaccg aggaagactg ggacagcgtg ctgtcagtgc acctgcgcgg
     9721 cgcgttcctc atgtccaagg cctgccagaa gtacatgacc gaggcgacgt tcgggcgcat
     9781 cgtcaacttg tcgtccacgt cggcgcaggg caaccggggg caggccaact acgccgcagc
     9841 caaggccggc atgcagggct tcaccaagac gctggcgatc gagttgggca agttcggcgt
     9901 gacgtgcaac gcgatcgccc cggggttcat ccagaccgag atgaccaagg cgacggctga
     9961 ccgcctcggg gtgtcgttcg aggacttcat cgcgttctcg gccaaggaga tcccggtcgc
    10021 ccgcgtcggt cagcccgagg acatcgccgc caccgtctcg ttcctcacca gcgacgaggc
    10081 cggtttcgtc aacggccagg tcatctacgt cgcaggcggc ccgaaggact gaccggcctt
    10141 gacacccgac tcggcagccg actgaccggc gccgacacag agccaggggc ggggcggcat
    10201 accaccgggt gtgccgcccc gctcccgtca cgcagcggga ggatcgggcc cggcggcctc
    10261 gcccgccttg cccccgtgac ccggcgccgc gagcgcgcga tcgtaggaac ccaggcgtcg
    10321 atcccagtcg actccatcgg acgtcacctg cccgaacagt cggtcgcggt gcaccaccgt
    10381 gtgatgtcgc ccgcacagca gggccgcgtt ggacaactgc gatacgccgc cgtcgagcca
    10441 gtggatcagg tggtgggcat cgcaccaatg cgcggggatc gaacattccg ggaacgaaca
    10501 cactcgatcg cgcagccaca acgccgccgt ctggccagca gtgaagagcc tggtcgcccg
    10561 cccgaggtca agcacttcgc cctcgccgcc gagcaccaca gggatcaccc cggcatcgca
    10621 ggccagcttc cgcaccgtct cgggcgcaat cagctctccg gcctcgctcg accccagaac
    10681 ctcccccgcc cccagtcggt tgctcaggtc ggcgaagggc atcgtcacca tcagcgtcga
    10741 cttgaccccg ctaggaggta tgccgcccgc agccgtcgcg cggcggcata cctcgatgag
    10801 ggcctcgccg cgacggcgct cgggtggacg cggatcgcgg gctccgtctg gcccaggcac
    10861 gggagccgag agcgggccga tcgcggcctc caacgccgcc ttgccctggg cgtcgacgac
    10921 cagccgatag gtgaacgcgc cgagcacgtc gccgaccgcg cggtccagtg cgaccagacg
    10981 cccggcggca tcctgcactt cctggaacac tcctgggtcg ccgaacttcg ccaacagcgc
    11041 cggccgcagc cgacggacgc cccgacgccc ttgctcagca cccatctgca cgagcccgtc
    11101 gagcacggtg ggtcgcgcct cgttgcgcag catgggtcgc aatgcctgga actcggacac
    11161 cacgccgagc cccacaccca ccgaaaccgt gccgccctcg accgccgcgc gcacagtcgc
    11221 cagctccgga agacgacact cctgcaccaa ccgggccact tgacccgccc cttcggcggc
    11281 cagtgaggga gcgtgtcgag ccacccactg agtgctcgtc cccgcctggc tggccgaaat
    11341 ctcacctctt gagtcagcct ccgcgaccac ggcgaccacc gcccccgcag cccgggacgc
    11401 cagtgcatcg agctcaccga ccaggaggcc caactcggca ccggcgacct ggcacagcag
    11461 cccggtcaac ccgtcgaggg ccgaccgcgc agccacgaag gcggcccgtc ggtcggcgac
    11521 cgacgcaccg cccgacacgg tggccgacgt gctgctcatg acctcaaact agccaggggg
    11581 cctgaccctc ccccgccgct cagcccgctt atccacaagc gtcgaggtca caaacaggtc
    11641 acagttctcc tagggtttgc accacgccgc tcttccgagc ggtgtcagcg cgccgccgac
    11701 cctgacgtcg acctgacacc cgccacccgt ccgcaaggga ccagacccgt gatcctcccg
    11761 aaggtgcgag accctcgcct cgtgacgatc cgtcgtggcg ggctcctgac cgacccggat
    11821 catcacctcc tggcgttgtg ggcagccgcc tgtgccgagc acgtccttga cctgttcgag
    11881 gccgaatgtc ctagtgatct ccggccccgg cacgcgatcg ccggcgctcg ggcctgggct
    11941 gcgggcgagc tcgccatgat gcagtccagg gcagccggtg ggcacgcgat gggggccgcc
    12001 agaccgttga gcggggcagc ccgtttcgcg gcgtatgccg cgggccaggc agcctgcatc
    12061 ccccatgtcc ccgaacacga cctcggcgca gccgcctacg cgatcaaggc agcccaggcc
    12121 gccgccgcgg ctggtgacga cggcgaggcc gccgcccgtc gcgagtgcca gtggcagctc
    12181 gaccaactgc ctgacccgat ccgggcgctc gtcctcgacg accaggcccg acgcaacccc
    12241 atctgctggt cggtgttcac ggagcccggc ccgctggctg ctggccccac ccacccctcg
    12301 ggcgggctac aacggtgagc gggcggcaaa ccccaagggt atgccgcccg ctccagtccg
    12361 tgcgtgcccg cggtcagttc tcggtcgact ccacggcagc ctccgccacc ggggcctcga
    12421 tgacctcggg cgccgccgac tcgacagccg ccgactcgac aaccggagcc tcgacgaccg
    12481 ctgccgtcac ctcgaccgga ccggttgtgg gcgcggaggc ctcagcagcg gccttcttgc
    12541 ctgccgactt cttgtctgcg cccttggcgt cgccggcctt cttgcccttg gccttggccg
    12601 cgcccttctt gggctcgacg acgaggatcg gcagcgtgtg cgcgtggtcc gggacgtagc
    12661 ggttggactc gcgcggcggc cggtgatagc cgacggcaac cggacggccc gggatctcga
    12721 ccgccttggg ctgctggtcc tcgtaaggaa tcgtcgagag caggtgctgg atgacgttga
    12781 tccgcgaggt cttcttgtcg tcggactcga cggtgtacca cggcgagtcg gacaggtcgg
    12841 tgaactgcat catcgtgtcc ttggcccggc tgtagtcctc ccaccgggtg atcgactgca
    12901 tgtccatcgg cgagagcttc caccggcgca tcgggtcgtt ggctcgcgaa cggaaccgag
    12961 cctcctgctc gacgtcgctc accgagaacc agtacttgcg cagcatgacg ccgtcgtcaa
    13021 tgagcatccg ctcgaacgac ggggcctggc gcaggaaccg ggcatattcg acgtcggagc
    13081 agaagcccat gacccgctcg acgccggccc ggttgtacca ggagcggtca aagagcacga
    13141 tctcgccggc ggccggaagg tgctcgacgt accgctggaa gtaccactgg gtcttctgcc
    13201 gctcggtcgg cgccggcaga gccgcaacgc gcgcctgccg agggttgagg tactccgtaa
    13261 tgcgcttgat cgacgagccc ttgcctgccg cgtcgcgccc ctcaaaaagg acgacaagac
    13321 ggctgccagt gccgatgacc cattgctgca tcgtcaccaa ctcggcttgg agtcggaaca
    13381 actccttctc ataggtcgcg gtgtccagct tgagggtccc gtttgcggtt cgcttcgcca
    13441 tggcgcgcat tgttccactc ccggtggtcc cctgtcgcgg ggcgtctcgt atgccgttca
    13501 tctcgcgttc acccgtgcct caccccttgg cgacgcgcgg gggtagcgct tacactccgg
    13561 ctcgatccgg cccggaacac gggcgcgctc gccgtggttg tcgtgtcaac cacattccga
    13621 cgaggagcct catgtccgag ttcacgcccg cgcctggcac agtgacgatg ttcacgacaa
    13681 cgtggtgtgg ctactgccgt cgcctcaaga gtcagatgga acgcgagggc atcggtttca
    13741 ccgaggtcga catcgaggtc cggcccgagg ccgctgacgt cgtcatgtcc gtcaacggtg
    13801 gcaaccagac cgtgccgacc ttgatctttc cggacggcag cgccgccacc aacccgtcga
    13861 ttgggcaggt caaggctcgg ctcgcctagg tcgtcgggcg ccagccctcg atgaccgcgt
    13921 gccccgccgc cacctcaaga tcggcaccga gccaccgttc gatgattcgc cgggcgatcg
    13981 agatgctgcc cggcacccgg atcgcccccg accgcagcag cgcggcatac tccgcccgcg
    14041 tcacccagcg cacctcggcc atctcggtcg ggtcgtgggt gagtgcagtg gtcaccgcgc
    14101 gggccgtgaa gccgagcatc agtgaggacg ggaacggcca cggctggctg ccaaggtagc
    14161 tcacctcggt caccacgacg ccggactcct ccaggacctc ccgcgccacg gccgcctcca
    14221 gcgactctcc cggctcgacg aacccggcga gcacggagaa ctggttcgcc ggccacgtcg
    14281 cgccgcgccc caacagcaat cgcccgtcat catcgatgac cgacatgatc accgagggat
    14341 cggtgcgcgg gaagtgctgg ctgccgtcgc gcgtgcaccg ccgcacccag ccggcggagg
    14401 ctggctcgct cggcgcccca cagcgcgagc agcggtcgtg actgcgatgc cagttcgcca
    14461 gcgcgagcag ggtcgcggcc aggctcgcgt ccagatcggg cagcaacgcc ccggccgctc
    14521 gcagggtctg ccagcccgcg tcgccgtcgc cgtccgtggg tgcctccacg actcccaggt
    14581 atgccgtccg ctcaccgtcc tcgcccgggt gcagaccgag gaagaccgcg agcaacccgc
    14641 catcggctgc agagggtgag cgaagcacca agccgagcgc tccggccacg ccatcggccg
    14701 gcgccggcga ggggtcagcc gttggggcca tcgagtgcgc cggcactggt gcaacgagca
    14761 cgcgatcacc acggaccgcg accacccggg tccccgcgtc cgccaggagt cggtcgagca
    14821 ggtccgcgtc ctcgcgcaga tgagcggccc gatccggtcc gccatgcgag agcagcaggc
    14881 cccgcaggcc cggcgtctgt tccatccgag cagtcaagca cgggcgaggc gtctcgcacc
    14941 ggcgacattc acggcatacc gtgtgagcgt gagtcccgag agccgaagcc cggcgttcct
    15001 cgccgccctc gccagcgcgg ccgtgccggg gctcgatccc gtctcggcgc gcgcggtggc
    15061 gcccggcccg gccggtgagt tcgacgcagc gatcgtcatc gatgagcagg accggccgtg
    15121 gcttgttcgc tgcccgcgga cgcctgcggc cggagcgcgg ctggagtcgg ccgcggcgct
    15181 ggctgccctg ctggcccggc ggttgccgtt ccaggtgccg gtgcccaagg gtttcgccgc
    15241 gctccccgac ggacgggccg gcgtctatcc cgtcctgccg ggtcgaccgt tggcgttcga
    15301 cgcgctcccg gccggccctg ggcttgccgc tgacctcggg cgggccatcg cggcgatcca
    15361 caacgtcgat cggggcgtct tcgacgaggc tggtatgccg tcctacgacg ccgactcgta
    15421 tcgcaccagg cacctggccg accttgatcg agccgccgcc acggggcatg tccccacggc
    15481 cttgctgtcc cgctgggagg gggcccttga cgacgtcacc ttgtggcgct tcgctccggc
    15541 accggtccac ggtgggttga ctgggtcgca ggtgctggcg gggttcgact ccgatgaaga
    15601 cgcagccacg gggcgggttc gggccgtcgt cggttgggag gacgccaagg tggccgaccc
    15661 tgccgacgac ttcgccgcca tcgtcactct ggctgggccg gctgccgtcg agtcggtgtt
    15721 ggaggcctat gccaactcac ggatcgagcg ccccgacccc catctgcgcc gacgggcgcg
    15781 gctggccgct gagttcgcgc agctcaccaa cctgctcaca gcagtgaccc tcggcgatcc
    15841 cgaccgagtg cgggcgatgg cccgacgcct cgtcgagttg gagcggcggg tcaccgacgc
    15901 cgacttgaag gcgccggagc cggtctccgt ggtcccccgg gaggtcatcg ttccgaagac
    15961 ggtggcagcg gcaagcccgg ccgccgcgaa cgggtccggc tcgggtgaag acaccgtggc
    16021 cgtcgacccg gacactctct tcgatgacac ggcgcccctt catggcactg cggctcacga
    16081 tgactcggtg actcacgatg actcggtcac tctcgatggc acagtgactc tcgatgacgc
    16141 tgcggctctc gatgacaccg tggcgatgga cccgcggacc ctcttcgatg ccaccgagga
    16201 gatccctacc gcccacggcc ctgcttcgcc gtcaaggcct gacgcgccgg caacgccaga
    16261 cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngccggcaa cgccagacga
    16381 gccggcctag tcggcgatcg accccaacaa gcggatgagc tcggcctcgt ccggcaacgt
    16441 cggccgaacc gtcgtccccg tccgcgcgta gtagaacgcc gcatccacct gatcgggcaa
    16501 caatccacga agccgcgcat aggccagcgc gtacgcaccc acctgcaacg cccgatgcgc
    16561 ggcgtccgcc cccgacggtt gtggcccggt cttccagtcg acgaccgtga ccccgccgtc
    16621 cgctcgcccg aacaccgcgt cgatgcgccc gcgcacagcg atgccgtcga tcaccgtctc
    16681 gatggccact tcgacatcta tcggcgtgcg ccacgcccac tcgctggcca ggaaggcctc
    16741 cttgagctgc ggcaactcgg cgtcggagcc cggatcgtcg tcggcggcac cggggagctc
    16801 ggcggaatcg agcaaggtcg cccgcgcgta gtgctgctcc acccacgcgt ggaaggccgt
    16861 cccacgccgg gccgcaagcg cgggctcggc aggcatcggt cggcggcgct cgccagcaaa
    16921 tcgctcagga tcctcggcca gccgcacgat gtccgacgct gacaggtgcc gtggcacctc
    16981 gacgacctgg gggcccgtct cgcgggccaa cgcccgctcg gcgagcagca ggtcgacatc
    17041 cgcggcatac ggatcgtccg cgccgccacc tcggggggcg agcagggccg cgcgcgggta
    17101 tgccgccacc ccctccccag tcgccgcacc gatcgctccc gcaccgagcg ctcccgcacc
    17161 gatcgctccc gcaccgatgg atcccccacc gatcgtcccc acgtctgaag ttcccgcgtg
    17221 agccgccccg gcagccgctc gttcacgacg ggccatcgcg acgaccgccg cggcccgctg
    17281 ggcgtcgtcg aggcgactct gatcgggcgg agtcggccac aacacgcgca ccgtctcgtc
    17341 agcgaccggg ttggtgccga ccgcagccgg gtcgggcatc gcatcccagt gcgcgacccg
    17401 gacaccgagc tcgtcacgag cccgaacgac ctcacggagg aacctggagg tgaccttgtg
    17461 ccgctgggtg ttggtccaca cgctcgcggt gagcagcagt cgcctccggg ctcgagtgac
    17521 cgcgacgtag gccagacggc gctcctcatc gagatcgcgc agcccctcct cgacccggaa
    17581 ggccgcgacc cgttcgctga gagccttact gtcggaagcg ccctccacct gcagcgtcgg
    17641 caacccgtcc cggtcgccgc gcaaagcgaa cggaaccccc gcgagcccga cgagccagcc
    17701 tcccggggtc gggtcgggaa ccacccagcg agcaccgttg tgcttcggcg tgggcgagcc
    17761 ttcgcgggca gggaaggtgc cctccacgag gcccggcact gccacgacgt cccattccaa
    17821 gcccttggca gcgtgaatgg tgaggatctg cacggcctcc gcggacggct cgagggtggc
    17881 acgttccagg ccgcgctcct gtgcgacggc agcatcgagc caggcgagga agccgatgag
    17941 ggtggggcgg tcggcagacg aggcgaagtc ggcagcgacg tcggcaaagg catccaggtg
    18001 ggcccgcgag gtgtcgggcg tgtaacccgg gcgcgagagc acctcgacgt ccagcccgag
    18061 cgctcgttcg gcctcaccga caaggtccgg tagcggcagc gcaccgagcc gtcgtagccg
    18121 ccgcaaggcg gtcgacagcc ccttgagccg aaccagcgcg cccgcaccga cgtactgacc
    18181 ctcggtgcca cgccacacct cgggcggcag atcgtcgagc gcctcgacga tgctcggctc
    18241 gtccaacacc tcgggcgcga ggtccacgag cgcatcggcg ggaccttcga cgccgtcgtc
    18301 ctcgtcacga tgtcccgggt cgaggtcgat cgccagccgc ccctgcgact cggccttgac
    18361 gaccttctgg cggaacctcg cccactcgtg cagcccgtcg aggtcggcgg cccccaaccg
    18421 gcacatcggg ccggtgagca gacgcatgag cgcatcaccg cggctcgggt cagcgatcgc
    18481 gcgcaacgcg gagaccaggt cgaggatctc gggggtcagc agcaacccgc cgagaccgac
    18541 gacctcgtag ggaacgccgg cggcgtcgag cgcctccatc accggaccga actgggatcg
    18601 cttgcggcac aacactgctg ccgtcgtacc cgcgcggctc gtcgcgccgg ccagccagtc
    18661 ggcgaggtat gccgcctcct cgacgtgagt gccgacgcgg gcgacgacga cgtccccgac
    18721 gcccgcgtcg ggacggaccg cgaggggtgc cacatcgacc cgcgcggacg cccgaagggg
    18781 tgccgcgagg tggttggcca ccttgaggat ggatgcgtcg ttgcgccagg tcgtcgccaa
    18841 ttgccgcaca ggagtgggcg acccatcggt gaactcggcc gggaaacggg tgagggtcgt
    18901 ggcgctcgct ccgcgccagc cgtagatcga ctggttgggg tcgccgacgg ctgtcaccgg
    18961 gaccccgctg tccccgaccg cgaagagcga ccgcagcagg acgagttggg cctcggaggt
    19021 gtcctggaac tcatcgagca gcacgacccg gaatcgggcc cgctcgccgg ccccgatgtc
    19081 ggggaacccc accgccaacc gggctgccag cgccacctgg tcggcaaagt cgacggcatc
    19141 acgtgagcgt ttcagggagc cgaaggcgtc gacgaccggc aacagggccc ggcgggcccg
    19201 cagcgtgccc cgcagcgtcg ccaggtcagc gggcagcgtc ttcgccgtgc cggccagcgg
    19261 ggtcgcgtcg atcgcgcgaa cgatccggtc gaggacctca cgcagcgtgc cggtgtcgac
    19321 gagatgctct gccagctcac ccgcgaggtc gacgaccgcc ccgatggcgg tcgattccgc
    19381 cttgcccatc tcggacatgt ccccgtcgta gcgggagacc acctcggcgg catactgcca
    19441 ggcggcggct tcggacagca gtcgcgagtc gggctcgtag cccagccgca gggcgtgctc
    19501 gcggaccagc cgaccggcgt acgagtggta ggtcgcgacg gtcggcgtcc caccgaggac
    19561 ctcggcaccc tcctcgtcgg gcgcgggcgt ccagagcccc gcagcctgca ggcggcgcag
    19621 ccgttggtcg atccgctcgg cgagctcggc ggccgccttg cgggtgaacg tgagcccgag
    19681 cacctgatcg ggagcaacga gcccgttggc cacgagccac acgacacggg ctgccatcgt
    19741 ctcggtcttc ccgctaccgg ccccggcgac cacgagcatc ggccgcaacg gcgcctcgat
    19801 gaccgcggtc tgctccggtg tcggggcgtg ggccaacccc gccgcgcgag cgacctccgc
    19861 tgcactgtgc cgaaccgatg ccatcagatg ctctgccctt cgggccgggc cgggcaggcc
    19921 gcgacgaccg gacacatccg gcaatgctcg cccggcgtcg cggcgaactg ccccccgccc
    19981 atgcccgctg cagtctcctc gacgaggcga ctggcccagc cggggtcatc gtcgtcagcg
    20041 agcgcgggct gcccctgcag ggtgaccgat ttgttcgacg ccttgcccaa ctgcagcaac
    20101 gcggccccgg ccgatcgggt ccccaggtcg ccgaaggcgc cgtgctcgac cgctgcctga
    20161 tagaccccga gttgggggtg gcgagccaac tcggccgcgg tcggcttgct cgacccggtc
    20221 ttgtaatcaa tcacccgcaa cgacccgtcg gtatgccgtt cgagccggtc cacgcgcccc
    20281 ttgagcaccg cacgccccac ctcgaccagc gcgtcgacct cgaccccgac ccgctcccag
    20341 ggggcggctg ccggcgagac gaagtaggcc gcggcgcgcc cggccatggc atgagcctcg
    20401 gcgcgctggc gctcctgcac ccacccggga gtgaggccga gttgtgccca gcggcgctcg
    20461 atctcggcct gcagggtcgg ggcatcaacg tcgccgagct cggcgacgac gtcgtgcacg
    20521 agggtgccga tcgatgccga ggcactcttg acgccgtcgc cgccactggc ccgcaacagc
    20581 caagccaact cgcacttggc gaactgctcg accttggacg gagagacccg cacggcctgg
    20641 tcagggccac gccggggacg atcgtcggcg gcggggacga gggaccacca ctgccccggg
    20701 tcggctccgg tcacgccatc gtcagcgagc cgcgccaact gccgcccggc ccgggcggcc
    20761 gctacctcgt cggtgaccga cgccgccacc tgacgcaggg caccgacgac tccggcggtg
    20821 gtcagcgcgc gaccgaacgg tgtgaagggt cgccctaggg cgccgtctgc gtcggccggc
    20881 aacgggtcga gcaggtcgag atagaccgac ggctgttcgt cgtcgttgcg cacggcgctg
    20941 acgatgagcc gctcgctcgc ccgcgtcacg gcgacgtgga acagccgggt ctcgtcgtat
    21001 cgcaccgcgg cctgcgccgc acgcaccgag ccacctcgac cagtgacgac gtcgacgagg
    21061 tgctctgagc cgagcagcga cccccgcaac cgcaggtctg gccagacgcc ctcttggact
    21121 ccagacacac acaccaatcg ccactccctc ccggcagcag cagcgggggt gagcaccgca
    21181 accgtgtcga ccgcaggagc gcgcagcgcg agggtgtcgc caggcacctc ttcgccgagc
    21241 acatggtcaa ggaaggcatc cggcccggag cccggcagcc ggtcggcata ccgggcagcc
    21301 gcgtcgaaca gaccaaccac cgcgtcgagg tcgcggtcgg cccggcctcc gcggctcccg
    21361 ccgtcgagcg ccgcgcgccg ccaactcgcc gcgacgccaa gggccgacca gatcgcccac
    21421 aacacggatt cggggctgac ctcgggggcc cagccagccg cgtcatcggt ccgccgggca
    21481 gccccgacac cggcgaggat cgcccgggtc aaccgccgca ggggctcccc gtccacaccg
    21541 gcctgggcac tgagcaccgg gtcgccaagg gctgccgcca gcaactcgtc gctcgaccgt
    21601 cgtcccccgc cgtcgagctc gatccggcgc agcgcccggc gcaaccggcg caacgacacc
    21661 gcgtcgcccc cacccaacac cgacaagcag aggtcgacgg cctccgtggg cgcgatcatc
    21721 gcgtccgggt cgcgggccag gcgcagacac aggtcgagca gcgcgagcag cggccggacg
    21781 gcgacctcgt cgcgcaccgg gacctcacca tcgggcgggg ccagaggcac cccggcggcc
    21841 gaaagcaccc ggcgcagagt ccccgcgcga ccacggcctc ggacgaggac ggccatgtcg
    21901 cgccacggca taccctcctg caggtgcgcg cgacgcagcg tcgaggcgat gaaccccgcc
    21961 tcctgggcga ccgagcgaag cagcgccacc tgcacctcac cgccgtcgcg aacgggctgg
    22021 gacccgcgcc ggtccgcgcc gccggtcgcc ccgatccggc gcacaacctc gctcgccgcg
    22081 tcgtggagcc tggtcggtgt ccggtatgcc gtgcgcagca ccaccgtggg cgcatcgcgc
    22141 agagcgggcc accggtccca ccacaacagc cgtgggtcgg ccccgcggaa ggtctggacc
    22201 gcgctgtcgg ggtcgccgat gaggatgatc tgcattccgg gatgggcgat ggttcgaagg
    22261 agtcgagctg cggcccaagt gagctcttgc gcgtcgtcga cgacgatcag ccggatctgc
    22321 tcgcgcaccc gctcgagcgc ctcggggtcg tcctcgagca ggtcagcagc ggcggtgagg
    22381 atccaggcag ggtcgtaggc acccggctgc gacagcgccg tcacctcgtc gtattcgtcg
    22441 aggaccgctg ctgccgacac ccactcaggg cggtcgtgct ggcgtccgag ctcggcgagg
    22501 ttggccgcgt cgactccgtg ctcgacggcc cgcatgagta gatcacgcaa ctcggctcgg
    22561 aagccccgcg tgcccaaggc agcggccacc cgctccggcc attgcggccc gccgccgctc
    22621 gccacgtgac ccgcgaggag atcgcgcagg acgacgtcct gctccgggcc gctgagcagc
    22681 cgcgaagccg gcgcaccgcg aagggctgcc tcgcggcgca gcaccgcgaa cccgaacgcc
    22741 tgatgcgtgc gcgcgagcgg ctcggtcgac gtgcgcgcca gcctcgcggt cagccggtcg
    22801 cgcagagcag cggcggcgag ccgggtcgga gcgaggacca ggcactggtc gggggtgagc
    22861 cccagccggt cgacccgatc gaggaccacc tcgacggcca cggtcgaggt gcccgtgcca
    22921 ggcccgccga gcacccgtgc gaccggggcg tccagcgcga gggcagcctg ctgctgcgcg
    22981 tcgagggtgg gcgcctcgac gacgagcgct ggtgcacgcc gcagggtgag catgcactca
    23041 tcccatcacg ggcgaccgac acccctccgc tgcctccccc ggagatagcg ctcgcacggt
    23101 catctttgcg ggccccagcc ccagaccacg acgg
//
LOCUS       DAOMZY010000014        19715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_2431_length_19715_cov_5.548118, whole genome shotgun sequence.
ACCESSION   DAOMZY010000014 DAOMZY010000000
VERSION     DAOMZY010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 19715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19715
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2431_length_19715_cov_5.548118"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="PLK64_01770"
     CDS             <1..914
                     /locus_tag="PLK64_01770"
                     /inference="COORDINATES: protein motif:HMM:NF018657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HPV78497.1"
                     /translation="CPASPIGSPKPPNARALRCLELQAVTAAPAAVLKATRDTANRLA
                     DGLERFVEGAESVEAAPAWARVGSPPATPFNAAITAERAWAWLPMPVAASSALRRATG
                     STLNDVLMAATAGALRDWLAENDALTTDPLIAMVPMSIRGQGHSVGSNQIMLTLCPLP
                     THLDTPRERLAFAHSAMDHAKATPTYPSTLMSDLAAVAGPTVTQLLTQAAAMVRLADR
                     VQLPFNLMVSSVPAPAREFIVGRGARVIGSFPFPPLSDGMGLSVCAQGYPGALDIGIG
                     SCSDLLPDPWRVRDLLEQAHDDLIASA"
     gene            complement(1044..1631)
                     /locus_tag="PLK64_01775"
     CDS             complement(1044..1631)
                     /locus_tag="PLK64_01775"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV78498.1"
                     /translation="MSAPQAPQALVRRVDERSVDVVCDLWAQQREERAPTELGLRRVT
                     ADVVRAALLRPETVAFAAYLNGAAVGYVVLSDCSLNPFSDAACVSVDQIYVAKDARKK
                     GVARALMTAVATFADRHGADQIACSVPSAVRDSNRFFARLGFTPHTIRRVTLTAALQR
                     RLVGDTAESRFALDQVLARRRVARIRAAQGRFAAH"
     gene            1833..2690
                     /locus_tag="PLK64_01780"
     CDS             1833..2690
                     /locus_tag="PLK64_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 family protein"
                     /protein_id="HPV78499.1"
                     /translation="MHVAFPGLGTVINIVAVVAGALLGMWVGHRIPERTRSVVTDCLG
                     LTTLLVAGLSTVSVTDAALRDAVGPGVPVLVVLGSLLIGSITGSTLRIEERLAGLAGI
                     LHERTARRRGGPATKADTGTSAAAAGDQTGTTDAVGDPETHADADAARERFIEGWLSA
                     SLLFCVGPLAILGSLSDGLGRGIDQLTLKSVLDGFAALAFASSFGVGVLLSAVSVGVV
                     QGLWTVLGVVLGSLLPDAHIAALTATGGLLLVGIGLRLLRIRDVPVGDMLPALLFAPL
                     LTQAIIALR"
     gene            complement(2743..3372)
                     /locus_tag="PLK64_01785"
     CDS             complement(2743..3372)
                     /locus_tag="PLK64_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPV78500.1"
                     /translation="MTIPTPAPPESPSPDRSGALRIVVAEDEALIRMDLVEMLAEAGY
                     EVVGEAGDGEAAVELVREARPDLVLMDVKMPVLDGISAAAIIGKESLAPVVMLTAFSQ
                     KELVERASEAGVMAYVVKPFTSADLTPAIEVAASRWAQLRTLESEIADLADRLETRKL
                     VDRAKGLLMTSLKVSEPDAFRWIQKTAMDRRLSMKEVATLVVEGLPSKG"
     gene            complement(3571..3653)
                     /locus_tag="PLK64_01790"
     tRNA            complement(3571..3653)
                     /locus_tag="PLK64_01790"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3618..3620),aa:Leu,seq:caa)
     gene            complement(3753..5228)
                     /gene="pyk"
                     /locus_tag="PLK64_01795"
     CDS             complement(3753..5228)
                     /gene="pyk"
                     /locus_tag="PLK64_01795"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPV78501.1"
                     /translation="MRRAKIVCTLGPATSSPARIRELVLAGMDIARLNLSHGEYSAHE
                     KVYRAVRQASDETGRAVGILVDLQGPKIRTGRFADGPVILKVGERFTITTLDVPGDQS
                     RVSTTYKGMPGDVHPGDLLLIDDGKVTLRAVEVTDTDIHTEVVEGGAVSNNKGINLPG
                     VAVSVPALSDKDKADLRWALSLRADMIALSFVRSAADIEDVHAIMDEVGVRLPVIAKI
                     EKPQAVENLPEILEVFDGLMVARGDLGVELPLEQVPLVQKRAVELARNRAKPVIVATQ
                     VLESMIENSRPTRAEASDAANAILDGADAVMLSGETSVGAWPIDAVRTMARIIENTEE
                     HGLERLAALHGQPRTIGGAVTLAAFEVGELINAKYLVTFTSTGDSAKRLARFRSKIPM
                     LAFTSSPATRSQLALVWGIETYLGPSVEHTDQMAEQVEQALLAEGRCVEGDLIVMVAG
                     SPPGIPGSTNMMRVLRVAGDTGHGNPFFHSHPGAEPVRSVQ"
     gene            complement(5380..6840)
                     /locus_tag="PLK64_01800"
     CDS             complement(5380..6840)
                     /locus_tag="PLK64_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HPV78502.1"
                     /translation="MADPQGFLNTRERELPTRRPVPIRLQDWREVYEDQDIHQLQRQA
                     GRCMDCGIPFCHNGCPLGNLIPEWNDLAWKGRWRDAIERLHATNNFPEFTGRLCPAPC
                     ETACVLGINQPAVTIKQVEVTVVEQAFAWGGVLPQAPERLSGKTVAVVGSGPAGLAAA
                     QQLTRAGHTVAVFERADKAGGLLRYGIPEFKMEKSIVERRIAQMEAEGTRFRTGVAVG
                     EDLTGEQLKARYDAVVLAIGATVPRDLSVPGRELSGIHQAMEYLPQGNRAALGQTVAG
                     QILATGKDVVVIGGGDTGADCIGTALRQGARSVTSLEILPQPPEERPAGQPWPTYPMI
                     FRVASAHEEGGERVYAVSTVEFVGEADGSVKALRVTDVALSNGRFEPVPGTERELPAQ
                     LVLLAMGFLGPQGEGLLEQLGVDLDERSNVVRNGSYMSSVPGVFVAGDAGRGQSLIVW
                     AIAEGRSAAHGVDAWLTGNPSQLPRPINPTDRQLLA"
     gene            complement(6833..11386)
                     /gene="gltB"
                     /locus_tag="PLK64_01805"
     CDS             complement(6833..11386)
                     /gene="gltB"
                     /locus_tag="PLK64_01805"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HPV78503.1"
                     /translation="MSHPRMPFSAQPDDVGLYRRDFEHDACGVAMVATLRGTAGHDIV
                     ELALTALRNLDHRGATGSDPLVGDGAGILTQVPDRFLREVTGLPLPPVGGYAVGTAFL
                     PTNGAERADAIERIENIATQEDLRVIGWRDLPVTPGLVGDAARVVMPFFAQVFVESTQ
                     GRTGLELDRAAFCLRKRAEREVGVYFPSLSARTLVYKGMLTTGQLEPFFPDLSDPRFE
                     TELALVHSRFSTNTFPSWPLAHPYRLIAHNGEINTVKGNRNWMRARESMLATDLIPGD
                     LQRLFPICTPGASDSASFDEVLELLHLSGRSLPHAVLMMIPEAWESHASMDPARRAFY
                     AYHANLMEPWDGPACVTFTDGTLIGAVLDRNGLRPGRYSVTDDGLVVLASETGVLDLD
                     PAKVVRKGRLQPGRMFLVDTEHGRLVSDDEVKAELAAAQPYQEWLHAGNIELDDLPER
                     EHVIHTAKSVARRQQTFGYTQEELRIVLAPMARTGGEALGSMGTDSPIAVLSDRPRIL
                     FDYFTQLFAQVTNPPLDAIREELVTSLGQVIGPESNILADTPAHARKVIVKFPVIDND
                     ELARIVRINHDGDLPGYATHVVKGLYPVSGGAAAMTARIEQIFAEVSAAISRGVRFVV
                     LSDRDSNADLAPIPSLLLTSAVHHHLLREKTRTQVGLLIEAGDVREVHHVALLIGFGA
                     AAVNPYLAMESVEDMVRSGALTGITPEKAVANLIKALGKGVLKVMSKMGISTVASYCG
                     AQVFEAIGLSQELVDRYFTGTVSQLGGVGIDVLAAESAARHSVAYPAEGIHQAHRTLW
                     VGGEYQWRREGEPHLFDPETVFRLQHSTRQRRYDIFKQYTSRVNDHSERLMTLRGLFD
                     FADPSVTGRAPIDISEVEPVSEIVKRFQTGAMSYGSISKEAHETLAIAMNRLGGKSNT
                     GEGGEDEERLLDPTRRSSIKQVASGRFGVTSLYLSESDDIQIKMAQGAKPGEGGQLPG
                     NKVYPWVAKTRHSTPGVGLISPPPHHDIYSIEDLAQLIHDLKNANPAARIHVKLVSEV
                     GVGTVAAGVSKAHADVVLISGHDGGTGASPLTSLKHAGAPWELGLAETQQTLRLNGLR
                     DRIVVQTDGQLKTGRDVVIAALLGAEEFGFATAPLVVSGCVMMRVCHLDTCPVGVATQ
                     NPELRARFSGKPEFIETFFEYIAEEVREYLAQLGYPTLQEAIGQVRSLVTDRAVSHWK
                     ASGMDLRPILAEVVDPSGGTAYQSVSQDHGLQKALDNQLVAMARPAIDNGEQVRGSIA
                     VRNVNRTVGTMLGHAVTSAHPHGLPDGTIELTLTGSGGQSFGAFLPAGVTLKLVGDTN
                     DYLGKGLSGGRLVVRPDRRAPLKAEQNVIAGNVIGYGATAGEIYLRGRAGERFCVRNS
                     GATAVVEGVGDHGCEYMTGGRVVVLGTTGRNFAAGMSGGLAYVLDLRRELVNAELVDL
                     APLRPGDVDAVREILEAHVQWTESAVATRLLANWEQEQSRFTVVVPRDYQRVLDVRAG
                     AQAEGLDPDGTEVWNRIMEASRG"
     gene            complement(11502..12473)
                     /gene="lgt"
                     /locus_tag="PLK64_01810"
     CDS             complement(11502..12473)
                     /gene="lgt"
                     /locus_tag="PLK64_01810"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPV78504.1"
                     /translation="MSAGALATLVTEIPSPSTGVWWLGPVPIRGYALSILLGIVVAVW
                     VAQRRIVSRGGTAGQVLDISAWAVPFGILGGRIYHLITSPQPYFGVGGNPWAAIRIWE
                     GGLGIWGAVALGALGAWIGCRRHGVAFSDFADAVAPGYLVAQALGRFGNWFNNEIYGP
                     PTELPWGLRIYEWDQQAGHAVIDSAGNPMVKGVFHPTFLYEALWCLALAAFILWFERH
                     RRLRRGQAFALVVIGYPLGRFFIELMRTDDANRVFGQRVNVWVSLLVIALGVVLYRRT
                     GRAVDPVEAREPVEPVEPGEPVLAGEAGEAQVSGPDAQNSPASRHTE"
     gene            complement(12470..13378)
                     /locus_tag="PLK64_01815"
     CDS             complement(12470..13378)
                     /locus_tag="PLK64_01815"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPV78505.1"
                     /translation="MSLQDPVGQPPEFPADPFAGAANVAGAAPVATPPPASSRRGVMG
                     VLVAAVAVLAIVVAVLLGQSMYRGGGTTASGAAPAANPAAPAATATAPAPAPAAKAPA
                     TATSKPAATGPVPTGAVGFGGPLVLNPSAPTGVPILDLYEDPQCPICQQFEAIFGTAI
                     AEVAKNNEAKVVVHTMTFLDRNLRNDSSVRAANAAFCAADQGKFREYMSATFAAQPAQ
                     EGVGWTEAQLATIAQSVSITGSALDTWKTCQQGLTYAAHIAALEINSERSGVNGTPTA
                     LLNGKKMNLSGLDAAGFRAAVKAGTA"
     gene            complement(13447..14172)
                     /locus_tag="PLK64_01820"
     CDS             complement(13447..14172)
                     /locus_tag="PLK64_01820"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPV78506.1"
                     /translation="MPRPDASAKLAATAPRRSRSGLAIIIAAVVFVAVVVAVVIGATT
                     SSKSSGGGGTAFPKGAASLGAPMVVNPEAPASVPVLDVFEDPQCPACATMDKVYGAAI
                     AELAKSNQAKVVIHTMTFLDTNLKNDASVRAAMGAYCAADQGKFLDYMSAVYVGQPQQ
                     EGVGWTAPQLEAFAMSARVGNLTTWQQCVKDKTYQAHTAAAAEAALKEGVNSTPTLRL
                     NGNKLTLTGNVNELAEAVKAATK"
     gene            complement(14218..14676)
                     /locus_tag="PLK64_01825"
     CDS             complement(14218..14676)
                     /locus_tag="PLK64_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HPV78507.1"
                     /translation="MTRERGLDLLGTLLRLVLGGVILVAGALKVTNLGQSALAVRAYQ
                     LLPYDLAGYVGYALPIIEIVIGLLLVLGLFTRISALLGALLMLAFVIGIASAWARGLS
                     IDCGCFGGGGTIGAEETAYPLELSRDVALLLAGAWLVRRPHTAYALDDRI"
     gene            complement(14673..>15403)
                     /gene="trpA"
                     /locus_tag="PLK64_01830"
     CDS             complement(14673..>15403)
                     /gene="trpA"
                     /locus_tag="PLK64_01830"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HPV78508.1"
                     /translation="GYLPVGFPSVEVSIEAMVAMVRAGADIIEVGIPYSDPLMDGPLI
                     QRATEAALEGGSRVDDVFTAVRAIADAGAAAYVMTYWNLVLKRGVARFAADLAAAGGS
                     GLITPDLIPDEAGEWLTASDALGLDRVFLVAPSSTLERLTVATGACRGFVYAASTMGV
                     TGTRETVGSAAQDVVRRTREVTDVPVCVGLGVSTRAQAADVAGFADGVIVGSALVRAL
                     GEGGIPAMEALTRELAAGVREGRP"
     assembly_gap    15404..15503
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15535..16737)
                     /gene="trpB"
                     /locus_tag="PLK64_01835"
     CDS             complement(15535..16737)
                     /gene="trpB"
                     /locus_tag="PLK64_01835"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HPV78509.1"
                     /translation="MTTPHGRFGDFGGRYVPEALIPALEQLDEVRRKAAVDPEFIGEL
                     DRLHRTYTGRPSIITEVPRFAEHCGGARVILKREDLNHTGSHKINNVLGQALLAKRMG
                     KSRVIAETGAGQHGVATATAAALLGLDCVVYMGAVDTKRQALNVARMRLLGAEVVAVT
                     NGSQTLKDAVNEALRDWVANVDSTHYVLGTVTGPHPFPEMVRDFHRIIGVEARAQVLD
                     LVGRLPDAVLACVGGGSNAMGIFHRFLFDDSVRLIGFEAGGDGIESGRHAARFSGGTR
                     GVLHGAATYVLQDDDGQTIESHSVSAGLDYPSVGPEHAHLFETGRAEYRPITDDEAME
                     AFRLLCRTEGIIPAIESSHALAGAMKVGRELGPDALLLVSLSGRGDKDVHTAARWFGL
                     LDDAEGMP"
     gene            complement(16734..17594)
                     /gene="trpC"
                     /locus_tag="PLK64_01840"
     CDS             complement(16734..17594)
                     /gene="trpC"
                     /locus_tag="PLK64_01840"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012245727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HPV78510.1"
                     /translation="MPATPTVLDEIIAGVRLDLADRVARTSLADLEARIATMPPALDA
                     EAALRAAPGIAVIAEVKRASPSKGALADIPDPAALATAYAAGGATAISVLTEERRFSG
                     SLADLDAVRATVRIPVLRKDFMVEPYQLLEARAHGADLILLIVAALSDIQLIAMREQA
                     QRLGMTALVEVHDAEETRRAVATGARVIGVNARNLKTLEVDPSAFARLRPLIPDEAVA
                     VAESGIRGVADVKGYAAEGARAVLVGEALVIGGDPAGAVASFAGVPVGGIPGQPQAST
                     TQASTTEGPR"
     gene            complement(17618..18049)
                     /locus_tag="PLK64_01845"
     CDS             complement(17618..18049)
                     /locus_tag="PLK64_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HPV78511.1"
                     /translation="MTIAEAASPRVGMGVRGLVQPALIAVGAAAGAAYLYAVDPNAPG
                     HYPPCPFLAVTGLWCPGCGSLRALHALLHLDVGTALARNPFAVLASIYIAWTFYRWAY
                     RRVTGTPLRLAPGWVITVVWVSIVAFWILRNVPGMTWLSPL"
     gene            complement(18061..18324)
                     /locus_tag="PLK64_01850"
     CDS             complement(18061..18324)
                     /locus_tag="PLK64_01850"
                     /inference="COORDINATES: protein motif:HMM:NF016394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD225/dispanin family protein"
                     /protein_id="HPV78512.1"
                     /translation="MSPPPQNNLVLAILATVLCCLPLGIVAILKANKVNTLWAQGDQA
                     GAHAAAAEAKKWSIISAILGIVVGIIYFVFVFLAAANGSVTVR"
     gene            complement(18576..18815)
                     /locus_tag="PLK64_01855"
     CDS             complement(18576..18815)
                     /locus_tag="PLK64_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HGxxPAAW family protein"
                     /protein_id="HPV78513.1"
                     /translation="MAGNDHHDNHGQSVAAWTGVAILTVAAAIMCWAVVVASTGLFIA
                     GAVLAVIGVVTGKVLAMAGFGVQKPGRNQVDSGVS"
     gene            complement(18885..19469)
                     /locus_tag="PLK64_01860"
     CDS             complement(18885..19469)
                     /locus_tag="PLK64_01860"
                     /inference="COORDINATES: protein motif:HMM:NF021079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trp biosynthesis-associated membrane protein"
                     /protein_id="HPV78514.1"
                     /translation="MTKRLVVLAVGGPLAVLLAALGQVWASGSSQDPVLGRAALSITG
                     TQMVPAALGLALVVGAALVAMLTGGPRIRTAAGVLMALAALAVVGLIGASLGSAGAAL
                     GRRAAELAGRTGSPVPVTSALGPWAWIAATGAGLTAVASIVATFSARRWAGLSARFDR
                     GGVDGATDRGARRSDWDDLTEGHDPTRADSGQGT"
     gene            complement(19466..>19715)
                     /locus_tag="PLK64_01865"
     CDS             complement(19466..>19715)
                     /locus_tag="PLK64_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HPV78515.1"
                     /translation="GLKRGVYGGVVGYLDFAGDLDMAIAIRTAVIRNGVAHVQAGAGI
                     VADSVPQLEHEECQHKAAAVLRAVQAAAGMQAAVAVSP"
BASE COUNT         3068 a         6765 c         6829 g         2953 t
ORIGIN      
        1 gttgtcccgc atcacccatc gggtcaccga agccaccgaa cgcacgggcg ctgcgctgtc
       61 tggagttgca ggccgtgacg gctgctccgg ccgccgtgct caaggcgacg cgcgacacgg
      121 ccaaccgact ggccgacggg ctcgagcgct tcgtcgaggg tgccgagagc gtcgaggccg
      181 cgcccgcctg ggcccgggtc ggctcgccgc ccgcgacccc gttcaacgcg gcgatcaccg
      241 ccgaacgcgc gtgggcctgg ctgccgatgc cggtggccgc gagttccgcg ctgcgacgcg
      301 cgacgggcag caccctcaac gacgtcctca tggcggcgac ggccggggcg ctgcgcgact
      361 ggctggccga gaacgacgcc ttgacgacgg accctctgat cgccatggtc cccatgtcga
      421 tcaggggcca gggccacagc gtcggcagca accagatcat gctcacgttg tgtcccctgc
      481 cgacccacct ggacaccccc cgcgaacggc tggccttcgc gcactcggcc atggaccacg
      541 ccaaggcgac gccgacctac ccctcgacgt tgatgtccga cctggccgcc gtggccggcc
      601 cgacggtgac ccaactgctc acccaggccg ccgccatggt gcggctcgcc gaccgtgtcc
      661 agttgccgtt caacctcatg gtctcgagcg tgccagcgcc ggcccgtgag ttcatcgtcg
      721 gtcgcggcgc ccgggtgatc ggctcgttcc cgttccctcc gttgtccgac ggcatgggcc
      781 tgtcggtgtg cgcccagggc tatccggggg ctttggacat cggcatcggg tcgtgctcgg
      841 acctgctgcc cgacccatgg cgggttcgcg acctgctgga gcaggcccac gacgacctca
      901 tcgcgtcggc ctagcgtcgg tcccccgtgc tcttcgggcc ggtgcgcgcg ccaaagatcg
      961 acgatctttc agttggtcga tgacatctcg tcgacctatc ggaaggtcga cgatgtttcg
     1021 ggtcggtggc ggtcagtgcg cggtcagtgc gcggcaaacc gaccctgagc ggcccggatc
     1081 cgggcgaccc ggcgacgggc cagcacctgg tcgagggcga agcgagactc ggcggtgtcg
     1141 ccgaccaggc gccgttgcag agcggccgtg agcgtgaccc gacggatggt gtgcggagtg
     1201 aagccgagcc gggcaaagaa ccggttcgag tcgcgcaccg ccgacggcac actgcaggcg
     1261 atctggtcgg cgccgtgccg atcggcgaag gttgccacgg ccgtcatcag cgcccgcgcg
     1321 acacccttct tgcgggcatc cttggcgaca tagatctgat cgaccgacac acacgccgca
     1381 tcgctgaacg ggttgagcga gcagtcgcta agcacgacgt aaccgacggc cgcaccgttg
     1441 aggtatgccg cgaacgcgac ggtctcaggc cgcagcaggg cggcccggac cacgtcagcc
     1501 gtcacccggc gaagccccaa ctcggtcggg gcccgctctt cgcgctgttg cgcccaaagg
     1561 tcgcagacga catccaccga gcgttcgtcg acccgacgaa ccagggcttg gggggcctgg
     1621 ggagctgaca ccgatgtcct tccacgaacg cgcgaccggt gtctggcccc tgaccggtga
     1681 gagcctctcg gccctgttga ctcactcttg ttcccgctga tgacgcactt tgttcccgct
     1741 gacaaccgac tctaatactc ctcggcaccc accggtaccc ccgggttggg cgctgcgaca
     1801 gccgctctca cgcgacccat agggtgtggg ccatgcatgt cgccttcccg ggcctgggca
     1861 cggtcatcaa catcgtcgcg gtcgtggccg gtgcgctgtt gggcatgtgg gtcgggcacc
     1921 ggatccccga gcgcacccga tcggtcgtca ccgactgcct tggcctgacg acgctgctcg
     1981 tcgccgggct gtcaacggtc tcggtcaccg atgccgcgtt gcgcgacgct gtcggcccgg
     2041 gagtgcccgt gctcgtcgtc ctcggctcgt tgctcatcgg gtcgatcacc gggtccacgt
     2101 tgcgcatcga ggaacggctg gccgggttgg ccggcatcct gcacgaacga accgcccgtc
     2161 gtcgcggcgg cccggcgacc aaggccgaca ccggcaccag cgcagccgcc gccggcgatc
     2221 agaccggcac caccgacgcg gtcggggacc ccgaaacgca cgccgatgcc gatgccgccc
     2281 gggagcggtt catcgagggg tggctcagcg cgtcattgtt gttctgtgtc ggcccgttgg
     2341 ccatcctggg cagcctgtcc gacggcctgg gccggggcat cgaccaactc accctcaagt
     2401 cggtgctcga cgggttcgcc gccctggcct tcgcgtcgtc gttcggcgtg ggcgtactgc
     2461 tctcggccgt ctcggtgggg gtcgtccaag gcttgtggac ggtgctcggc gtggtcctcg
     2521 gctccttgct ccccgacgcc cacatcgccg cgctgaccgc gaccggcggg ttgttgctcg
     2581 tcggaatcgg actgcggctg ttgcggatcc gcgacgtgcc ggtcggcgac atgctgccgg
     2641 ccctgctttt tgcacccctg ctgacgcaag cgatcatcgc gctgcgctag ccgcgctcgc
     2701 tgcgttcgcc gcgcgagcgg cacaccgcgt gggctgggtt gctcagccct tgctcggcag
     2761 gccttcgacg acgagggtgg ctacttcctt catcgacagg cgtcggtcca tcgccgtctt
     2821 ctggatccac cggaacgcgt ccggctcact caccttgagt gaggtcatga gcagcccctt
     2881 ggcccggtcg accagtttgc gggtctcgag ccggtcggcc aggtcggcga tctcggactc
     2941 gagggtgcgc agttgggccc agcggctggc ggcgacctcg atggccggcg tgaggtcggc
     3001 cgacgtgaac ggcttgacga cgtaggccat gactccggcc tcgctggctc gctcgacgag
     3061 ctccttctgc gagaaggcgg tcagcatgac gaccggcgcg agtgactcct tgccgatgat
     3121 cgccgcggcg ctgatcccgt cgagcaccgg catcttcacg tccatgagca ccagatccgg
     3181 acgcgcctcg cggaccaact cgacggctgc ctcgccatcg ccggcctcac cgacgacctc
     3241 gtagcccgcc tcggcgagca tctcgaccag gtccatccgg atgagggctt cgtcctcggc
     3301 cacgacgatc cgcagtgcgc cggatcggtc cggcgacggg gattccggtg gggcgggcgt
     3361 cgggatcgtc atggccagat cctaccgagc ctgcgcaacg ccgattgacc cagcccagga
     3421 cccctcggat gacttggcgg cctcgtcctc gcgggtgtgc gcaggcaacc ccggtccgga
     3481 cgagtgggcg ggcagccggg cctcacggcc gccgcgtcca ccacgatcgc aactacccaa
     3541 gctcaacgac acatctcaag aacacaacgg tgccgggagg gggattcgaa cccccacgcc
     3601 cttgcgagca gagcattttg agtgccccgt gtctgccatt ccaccacccc ggctgggtgc
     3661 ccgagaagca tacccacagc ctcccccaca ccaacaaacc gctcaggtat gccgcgtggc
     3721 cgggccgcgc ggcatacctg agtcgtcgtg cgctactgga ccgaacgaac cggctcggcg
     3781 ccggggtgcg agtggaagaa ggggttgccg tggccggtgt cgccggcgac ccgcagcacg
     3841 cgcatcatgt tcgtcgagcc ggggattccg ggcggggaac cggccaccat gacgatgagg
     3901 tcaccctcga cacagcggcc ttcggcgagc agggcctgct cgacctgctc ggccatctgg
     3961 tcggtgtgct cgacactcgg gccgaggtag gtctcgatgc cccagaccag tgccaactgg
     4021 ctgcgcgtcg cggggctgga ggtgaaggcc agcatgggga tcttggagcg gaaccgcgcc
     4081 aaccgcttgg ccgagtcgcc ggttgaggtg aacgtcacga ggtacttcgc gttgatcaac
     4141 tcaccgacct cgaaggcggc cagcgtgacc gcccccccga tggtccgcgg ctggccgtgc
     4201 agcgccgcaa gacgctccag gccgtgctcc tcggtgttct cgatgatccg ggccatggtg
     4261 cgcactgcgt cgatgggcca cgcgcccacg ctcgtctcgc ccgagagcat caccgcatcg
     4321 gccccgtcga ggatcgcgtt ggccgcgtcg ctggcctcgg cccgggtcgg tcgcgagttc
     4381 tcgatcatcg actcgagcac ctgggtggcg acgatgacgg gcttggcccg gttgcgcgcc
     4441 aactcgacgg cacgcttctg cacgagcggc acctgctcca gcgggagttc gacgcccagg
     4501 tcgccacggg ccaccatgag cccgtcgaag acctcaagga tctccgggag gttctcgacg
     4561 gcctgcggct tctcgatctt ggcgatgacc ggcagccgga ccccgacctc gtccatgatc
     4621 gcgtggacgt cctcgatgtc ggcggccgag cggacgaacg acagcgcgat catgtcggcc
     4681 cgcagggaca gtgcccagcg caggtcggcc ttgtccttat ccgacagtgc ggggacgctc
     4741 acggcgacgc cgggcaggtt gatgcccttg ttgttcgaca ccgcaccgcc ctcgacgacc
     4801 tcggtgtgga tgtcggtgtc ggtcacctcc acggcgcgca gggtgacctt gccgtcgtcg
     4861 atgagcaaca ggtcaccggg gtgaacgtcg ccaggcatgc ccttgtaggt cgtgctcacc
     4921 cgcgactgat cgccgggcac gtcgagggtc gtgatcgtga aacgctcgcc caccttgagg
     4981 atcactgggc cgtcggcgaa ccgaccggtg cggatcttcg gaccctgcag gtcgacaagg
     5041 atcccgacgg cccgcccggt ctcgtcggag gcctggcgta ccgcccgata gaccttctcg
     5101 tgcgcggagt actccccgtg ggagaggttg agccgggcta tgtccattcc cgcgaggaca
     5161 agttcccgga tccgggctgg gctggaggtg gcagggccaa gagtgcaaac gattttagcg
     5221 cgacgcatgt cgagcaacct taggggttag tgaagcggag cggggccagg tatgccggtc
     5281 ccggcaggtc tgatccggat cagacccctg ttctgatccg gatcagactc gacttgtgcg
     5341 agatatgcga gatatgtgga gagttggtgg tcgaccgggt caggcgagca actgtcggtc
     5401 ggtcgggttg atcggtcgag gcaactggct ggggttcccc gtcagccagg cgtcgacccc
     5461 gtgcgctgcc gagcggccct cggcaatggc ccagacgatg agggactgac ctcgaccggc
     5521 gtcaccggcg acgaagacgc ccggcaccga gctcatgtag ctgccgttgc gcacgacgtt
     5581 cgagcgctcg tccaggtcga cgcccaactg ctcgagcagc ccctcgccct gtgggccgag
     5641 gaagcccatg gccagcagga ccaactgcgc cggcaactcc cgctcggtgc ccggcaccgg
     5701 ctcgaaccgg ccgttggaca acgcgacgtc ggtgacccgc agcgccttga ccgacccgtc
     5761 cgcctcgccg acgaactcga cggtcgacac ggcatacacc cgctcgccgc cctcctcgtg
     5821 cgcggaggcg acgcggaaga tcatcgggta ggtgggccac ggctgccccg ccgggcgctc
     5881 ctccggcggc tgcggcagga tctccagcga cgtcaccgac cgcgcgccct ggcgcagtgc
     5941 ggtgccgatg cagtcggcgc cggtgtcgcc gccgccgatg acgacgacgt ccttgccggt
     6001 cgcgaggatc tgacccgcga cggtctgccc cagcgcggca cggttgcctt gcgggagata
     6061 ctccatggcc tggtgtatgc cgctcagctc gcgcccgggg acgctcaagt cacgcgggac
     6121 ggtcgccccg atggccagca ccacggcgtc gtagcgggcc ttcaactgct caccggtgag
     6181 gtcctcgccg acagcaacac cggtgcggaa ccgagtgccc tcggcctcca tctgggcgat
     6241 ccgacgctcg acgatggact tctccatctt gaactcgggg atgccatagc gcagcagccc
     6301 gccagccttg tcggcccgct cgaaaacggc gaccgtgtga ccggcccggg tgagctgctg
     6361 ggccgctgcc aggcccgctg gacccgagcc gacgacggca acggtcttgc cggacaaccg
     6421 ctcgggtgcc tgcggcaaca ccccgcccca ggcgaaggcc tgctcgacga cggtgacctc
     6481 gacctgcttg atcgtcacgg cgggctggtt gatccccagg acgcaggcgg tctcgcacgg
     6541 tgccgggcac aaccgcccgg tgaactcggg gaagttgtta gtcgcgtgca gccgctcgat
     6601 cgcgtcgcgc caccgcccct tccacgccag gtcgttccac tcggggatga ggttcccgag
     6661 cgggcatccg ttgtgacaga acggaatccc acagtccatg cagcgaccgg cctggcgctg
     6721 caactggtgg atgtcctggt cctcgtagac ctcacgccag tcctgcaacc ggatcggcac
     6781 ggggcgacgg gtgggcaact cgcgttcgcg agtgttgaga aagccttggg ggtcagccac
     6841 gggacgcctc catgattcgg ttccacacct cggtgccgtc cgggtccagg ccctcggcct
     6901 gggcaccggc gcgcacgtcg aggacccgct ggtagtcgcg ggggacgacg acggtgaaac
     6961 gggactgttc ctgctcccag ttggccagga gccgggtcgc caccgccgat tcggtccact
     7021 gcacgtgcgc ctcgaggatc tcccgaaccg cgtcgacatc gccggggcgc agcggggcca
     7081 ggtcgacgag ctccgcgttg accagctcgc ggcgcaggtc cagcacgtag gccaacccgc
     7141 ccgacatgcc cgcggcgaag ttgcgcccgg tggtgccgag cacgacgacc cgaccaccgg
     7201 tcatgtattc gcatccatgg tcaccgacgc cttcgacgac cgccgtcgcg ccagagttgc
     7261 ggacacagaa gcgctcgccg gctcgaccac gcaggtagat ctcgccagcc gtcgcgccgt
     7321 aaccgatgac gttgccggca atgacgttct gctcggcctt gagcggcgcc cgacggtccg
     7381 gacgcacgac gagccgccca cccgagaggc ccttgccgag gtagtcgttg gtgtccccca
     7441 ccagcttgag agtgaccccc gcgggcaaga acgcaccgaa ggactggccg ccggacccgg
     7501 tgagcgtgag ctcgatggtg ccgtcgggca ggccgtgcgg gtgtgccgag gtaaccgcgt
     7561 ggccgagcat cgtcccgacg gtgcggttga cgttgcggac cgcgatcgac ccgcgcacct
     7621 gctcgccgtt gtcgatggcc ggtcgggcca tcgccacaag ctggttgtcc agcgccttct
     7681 gcagtccgtg gtcctgcgac acgctctggt atgccgtgcc gccactgggg tcgacgacct
     7741 cagcgaggat gggacgcagg tccatcccac tggccttcca gtgcgagacc gcccggtcgg
     7801 tgaccaggga acgaacctga ccgatcgcct cctgcagggt cgggtaaccc aactgcgcga
     7861 ggtattcgcg gacctcctcg gcgatgtact cgaagaaggt ctcgatgaac tccggcttgc
     7921 cggagaagcg ggcccgcagc tccgggttct gagtcgccac gccgaccggg caggtgtcca
     7981 ggtggcagac gcgcatcatc acgcagccgc tgaccaccag gggtgcagtg gcgaagccga
     8041 actcctcggc gcccagcagc gccgcgatga cgacatcccg accggtcttg agctggccgt
     8101 cggtctgcac gacgatccgg tcgcgcagtc cgttgagccg cagggtctgc tgggtctcgg
     8161 ccaggccgag ctcccacggt gcgcccgcgt gcttgagcga ggtcaacggc gaggctcccg
     8221 tgccgccgtc gtggcccgag atgagcacga catcggcgtg ggccttggag acacctgccg
     8281 cgaccgttcc gacacccacc tcggacacga gcttgacgtg gatccgagcc gccgggttgg
     8341 cgttcttgag gtcgtggatg agctgggcca ggtcctcgat cgaatagatg tcgtggtgcg
     8401 ggggcgggct gatcagcccg acacccggcg tcgagtgccg agtcttggcc acccaggggt
     8461 agaccttgtt gccgggcagc tggccgccct cccccggctt ggcgccctgg gccatcttga
     8521 tctggatgtc gtcggattcg gacaggtaga ggctggtcac gccgaagcgt ccggaggcca
     8581 cctgcttgat cgacgagcgc cgagtcgggt cgagcagccg ctcctcgtcc tcgccgccct
     8641 cgcccgtgtt ggacttgccg cccaagcggt tcattgcgat ggcgagggtc tcgtgtgctt
     8701 ccttgctgat cgacccatac gacatcgccc cggtctggaa gcgcttgacg atctcgctga
     8761 ccggctccac ctcgctgatg tcgatgggcg cccggcccgt caccgacggg tcggcgaagt
     8821 cgaacagacc gcgcagcgtc atgagccgct cggagtggtc gttgacccgc gaggtgtact
     8881 gcttgaagat gtcgtagcgc cgctgccgcg tggagtgctg cagccggaag accgtctcgg
     8941 ggtcgaacag gtgcggttcg ccctctcgac gccactggta ttcgccgccg acccacaggg
     9001 tccggtgggc ctggtggatg ccttccgccg gataggccac cgagtgccgg gctgccgact
     9061 cggccgcgag gacgtcgatg ccgacgccac cgagttgcga caccgtgccg gtgaagtagc
     9121 ggtcgaccaa ctcctgggac agcccgatgg cctcaaagac ctgtgccccg cagtaggacg
     9181 caaccgtcga gatccccatc ttgctcatca ccttgagcac acccttgccc agggccttga
     9241 tgaggttggc gaccgccttc tcgggcgtga tcccggtcag tgcgccactg cgaaccatgt
     9301 cctcgacgga ttccatcgcg aggtacgggt tgacggcggc agcgccgaaa ccgatgagca
     9361 gagcgacgtg gtggacttca cggacgtcgc cggcctcgat gagcagaccc acctgggtgc
     9421 gggtcttctc ccgcagcaag tggtggtgga ccgcggaggt gagcaacagc gacgggatcg
     9481 gcgcgaggtc agcgttggag tcgcggtcgg agagcacgac gaagcgcacc ccgcgcgaga
     9541 tcgcggccga gacctcggcg aagatctgct cgatccgcgc cgtcatggcg gccgctcccc
     9601 cgctgacggg gtagaggccc ttgacgacgt gggtggcgta acccggcagg tcgccgtcgt
     9661 ggttgatccg cacgatccgg gccaactcgt cgttgtcgat gaccgggaac ttcacgatga
     9721 ccttgcgcgc gtgcgccggg gtgtcggcca ggatgttgct ctccgggccg atgacctggc
     9781 cgagcgaggt gacgagttcc tcgcgaatgg cgtccagcgg cgggttcgtc acctgtgcga
     9841 agagctgggt gaagtagtcg aagaggatgc gcggccggtc ggacagcacc gcgatcggtg
     9901 agtcggtgcc catcgacccg agggcttcgc cgccggtccg cgccatcggg gcgaggacga
     9961 tccgcagctc ctcctgggtg tagccgaagg tctgctgacg gcgagccacc gacttggcgg
    10021 tgtggatgac gtgttcgcgc tcaggcaggt cgtccagctc gatgtttccg gcgtgcaacc
    10081 actcttggta gggctgtgct gccgcgagct cggccttgac ctcgtcgtcg ctgaccaggc
    10141 ggccgtgctc ggtgtcgacg aggaacatcc gacccggctg caaacgtccc ttgcgcacga
    10201 ccttggccgg gtcgagatcc aggacaccgg tctccgacgc aagcacgacg agcccgtcgt
    10261 cggtcaccga atagcgccca gggcgcagcc cgttgcggtc aaggacggca ccgatgagcg
    10321 tgccgtcggt gaaggtcacg caggccggcc cgtcccacgg ctccatgagg ttcgcgtggt
    10381 aggcgtagaa ggcccgtcgg gcggggtcca tcgacgcgtg gctctcccag gcctcgggga
    10441 tcatcatgag gacggcgtgc ggcagcgatc ggccactgag gtgcagcagc tcgagcacct
    10501 cgtcgaagga cgccgagtca gatgcacccg gggtgcagat cgggaagagc cgctgcaagt
    10561 cgccggggat gaggtcggtg gcgagcatcg actcgcgggc ccgcatccag ttgcggttgc
    10621 ccttgaccgt gttgatctcg ccgttgtggg cgatcagccg gtaagggtgg gccaacggcc
    10681 aggaggggaa ggtgttggtg gagaaccgcg agtgcaccag ggcgagctcg gtctcgaacc
    10741 gagggtcgga caggtcgggg aagaacggct ccaactgccc ggtcgtcagc atgcccttgt
    10801 agaccagggt gcgcgcggac agcgacggga agtagacccc gacctcgcgc tcggcccgct
    10861 tgcgcaggca gaaggcggcc cggtccaact ccagcccggt gcggccttgg gtcgactcga
    10921 cgaagacctg ggcgaagaac ggcatgacga ctcgggcggc gtcgccgacc aggccggggg
    10981 tgacgggcag gtcacgccag ccgatgaccc tcaggtcctc ctgcgtggcg atgttctcga
    11041 tgcgctcgat cgcgtcggca cgctcggccc cgttggtggg caggaaggcg gtgcccacgg
    11101 catacccacc gacaggcggc aacggcaggc cggtgacctc gcgcaggaag cggtcgggca
    11161 cctgggtgag gatcccggca ccgtcgccga cgagtgggtc cgagccagtg gcgccgcggt
    11221 ggtcgagatt gcgcagcgca gtcagggcca actcgacgat gtcgtggcct gcggttcccc
    11281 gcaacgtcgc caccatggcg acgccgcaag cgtcgtgctc gaagtcacgc cggtagagcc
    11341 cgacatcgtc gggttgcgcc gagaagggca tacgcggatg cgacatagtg cctcaccgtc
    11401 ctggataggc gcccacaagg gcgcggatga cgcggctgga cggcgatggc cagcgaagga
    11461 aggatagcga acccgaaaac cgtcctacag tttgatacga ctcattccgt atgccgagac
    11521 gcggggctgt tttgggcgtc tggaccgctc acttgagcct caccagcctc accagccaga
    11581 actggctcac ctggctcaac cggctccact ggttcacggg cctcgaccgg atcgacggct
    11641 cgcccggtgc gccggtagag gacgacgccc agagcgatga cgagcagcga cacccagacg
    11701 ttgacccgct gtccgaagac gcggttggca tcgtcagtgc gcatcaactc gatgaagaag
    11761 cggccgagcg gatagccgat gaccaccagg gcgaaggcct gcccccggcg caaccggcgg
    11821 tgccgctcga accacaggat gaacgcagcc agcgcgaggc accacagcgc ctcatagagg
    11881 aaggtcgggt ggaagacccc tttgaccatc gggttgccgg ccgagtcgat cacggcgtgc
    11941 ccggcctgct ggtcccattc gtagatccgc agtccccagg gcagttcggt cggcgggccg
    12001 tagatctcgt tgttgaacca gttgccgaag cgacccagcg cctgcgcgac gaggtaaccg
    12061 ggtgccacgg catcggcgaa gtcactgaag gcgactccgt gccgccgaca gccgatccag
    12121 gcgcccaggg ccccgagggc gacggcaccc cagatgccca ggccgccctc ccagatgcga
    12181 atggcggccc acgggtttcc gccgacaccg aagtagggct gtgggctcgt gatgaggtgg
    12241 tagatgcgcc cgccgaggat gccgaacggc acggcccacg ccgagatgtc gagcacctga
    12301 ccggccgttc ctccgcgact gacgatccgt cgctgggcga cccacacggc gacgacgatc
    12361 cccaggagga tcgagagcgc atagccccga atggggacag gcccgagcca ccacacgccg
    12421 gttgacgggc tggggatctc ggtgacaagc gtcgccaacg cccccgcact cacgccgttc
    12481 ctgccttcac ggcagcccgg aacccggccg cgtcgagccc cgacaggttc atcttcttgc
    12541 cgttgagcaa cgccgtgggc gtgccgttga cccctgaccg ctcggagttg atctccaggg
    12601 cggcgatgtg agccgcgtag gtgagcccct gctggcaggt cttccaggtg tccagcgccg
    12661 acccggtgat gctcaccgac tgcgcgatgg tcgcgagttg ggcctcggtc cagccgacgc
    12721 cttcttgagc cggttgggcg gcgaaggtgg cactcatgta ctcgcggaac ttgccctgat
    12781 cggccgcgca gaaggcggca ttggcggccc ggaccgacga gtcgttgcgc aggttgcggt
    12841 cgaggaaggt catcgtgtgg acgacgacct tggcttcgtt gttcttggcc acctcggcga
    12901 tcgcggtgcc gaagatggcc tcaaactgct ggcagatcgg gcactgaggg tcttcgtaca
    12961 ggtcgaggat gggaacgcca gtcggagccg acgggttgag cacgagcggg ccgccgaagc
    13021 cgacggcacc agtcggaacc ggaccggttg cggcgggctt tgacgtggcc gtcgcgggcg
    13081 ccttcgccgc gggcgcgggc gcgggcgccg tcgctgtggc cgcgggagcg gccgggttcg
    13141 cagcgggtgc tgcgccagag gcggtggtgc cgccaccacg gtacatcgac tgaccaagca
    13201 ggaccgccac gacgatcgcc agcacggcga cggccgcgac gagcacgccc atcacgccgc
    13261 gacgagagct cgcgggtgga ggtgtcgcta cgggcgcagc gccggccacg ttggctgcac
    13321 cggcgaacgg atccgcgggg aactcgggtg gttgcccgac agggtcctgt aacgacatgc
    13381 cgcccattct ccctcatccg ggagggcgcc ccggcccagg cccgggcggg gctggtgcgg
    13441 cgcagactac ttcgtcgcgg ccttgacggc ttccgcaagc tcgttgacgt tgccggtgag
    13501 cgtcagtttg ttgccgttga ggcgcagcgt gggggtgctg ttcacgccct ccttgagggc
    13561 ggcctcggcg gctgctgcgg tgtgtgcctg gtaggtcttg tccttgacgc actgctgcca
    13621 ggtggtcagg ttgcccaccc gggccgacat ggcgaacgcc tccaactgcg gggcggtcca
    13681 gcccacccct tcctgctggg gctgaccaac gtagacagcc gacatgtagt ccaggaactt
    13741 gccctggtca gccgcgcagt aggcgcccat cgcggcccgc accgacgcat cgttcttgag
    13801 gttggtgtcc aggaacgtca tcgtgtggat gacgaccttg gcctggttgg acttggccag
    13861 ctcagcgatc gccgcgccgt agaccttgtc catcgtcgcg cacgcggggc actgagggtc
    13921 ctcgaagacg tccagcaccg gcaccgaagc gggagcctcg gggttgacga ccatgggcgc
    13981 acccagcgac gcggcgccct tggggaaggc agtcccgcct ccgccgctgg acttggacga
    14041 ggtcgtggca ccgatgacga cggcgacgac cacggccacg aagacgacgg cggcaatgat
    14101 gatcgccagg ccactacggg agcgacgggg agcggtggcg gcgagcttgg cagaggcgtc
    14161 tgggcgcggc atacctccca ttgtctctca tgtcaggcgc gcgctcgccg ggtgcggtca
    14221 gatccggtcg tcgagggcgt atgccgtgtg cggacgccgc accagccacg cccccgccag
    14281 cagcagggcg acgtcgcgac tcaactcgag cggataggcg gtctcttccg cgccgatggt
    14341 gccgccgccg ccgaagcagc cgcagtcgat cgacaggcct cgcgcccagg ccgacgcgat
    14401 cccgatgacg aacgccagca tgagcaaggc gcccaaaagg gcgcttatcc gggtaaacag
    14461 cccgaggacg agcaggagcc cgatgacgat ctcgatgatc ggcagcgcat agccgacata
    14521 acccgcgagg tcgtagggca gcagctggta ggcccgcacg gcgagcgcgg actgcccgag
    14581 gttggtcacc ttgagcgccc cggcgacgag gatcacgccg ccgaggacga gccgcaggag
    14641 ggttcccagc aggtccagcc cgcgctcgcg agtcacggcc ggccctcgcg gaccccggcc
    14701 gccaactcgc gggtgagggc ttccatcgcc ggtatgccgc cctcccccaa ggcgcgcacg
    14761 agcgccgagc cgacgatgac cccgtcggcg aaccccgcga cgtccgcggc ctgggcccgg
    14821 gtcgacacgc cgagcccgac acacacgggg acgtcggtca cctcgcgggt gcgccgcacg
    14881 acgtcctgcg cggccgagcc gaccgtctcg cgggtgccgg tgacacccat cgtcgacgcg
    14941 gcgtagacga agccccgaca agcccccgtg gcgaccgtca gtcgctcaag ggtggacgac
    15001 ggggcgacga ggaagacccg gtccaggccg agggcgtccg aggcggtgag ccactcgccg
    15061 gcctcatcgg ggatgaggtc aggggtgatg aggcccgagc cgccggccgc ggcgaggtcg
    15121 gcggcgaacc gcgcgacccc tcgcttgagc acgaggttcc agtaggtcat cacgtatgcc
    15181 gccgcgccgg cgtccgcgat cgcccggacc gcagtgaaca cgtcatcgac ccgcgagccg
    15241 ccctcgagcg ccgcttccgt agcccgttgg atcagcggac cgtccatgag cgggtcggag
    15301 tagggaatgc cgacctcgat gatgtcggcg ccggctcgaa ccatcgccac catcgcctcg
    15361 atcgagacct ccaccgacgg gaagccgacc ggaaggtagc cgannnnnnn nnnnnnnnnn
    15421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15481 nnnnnnnnnn nnnnnnnnnn nnnttcgcgg cgagagggct cgcaggggtg aggttcacgg
    15541 cataccctcc gcgtcgtcga gcagcccgaa ccagcgagcc gcggtgtgca cgtccttgtc
    15601 gccgcgcccg gagagcgaga cgagcagcag cgcgtctggc cccaactcac gcccgacctt
    15661 catcgcgccc gcgagggcgt gcgacgactc gatcgccggg atgatcccct cggtgcgaca
    15721 cagcagccgg aacgcctcca ttgcctcgtc atcggtgatc ggccgatatt cggcgcgtcc
    15781 cgtctcgaag aggtgggcat gctcagggcc gacagagggg tagtccaatc cggctgacac
    15841 cgaatgggat tcgatggtct gcccgtcgtc gtcctgcagc acgtaggtgg ccgcaccatg
    15901 caggacccct cgggtgccgc cggagaagcg cgctgcgtga cgaccgctct cgatgccgtc
    15961 gccgccggcc tcgaagccga tcagccgcac cgagtcgtcg aagaggaacc ggtggaagat
    16021 gcccatggcg ttggagccac cgccgacgca cgcgaggacg gcgtcgggca gtcgaccgac
    16081 gaggtcgagc acctgggccc gggcctcgac cccgatgatc cggtggaagt cacgcaccat
    16141 ctcggggaac gggtgcggcc cagtcaccgt gcccagcacg tagtgggtcg agtcgacgtt
    16201 ggccacccag tcgcgcaggg cctcgttgac ggcgtccttg agggtctggc tgccgttggt
    16261 caccgcgacg acctcggcgc cgagcaagcg catccgggcg acgttgagcg cctggcgctt
    16321 cgtgtcgacc gcacccatat agacgacgca gtccagcccg agcagcgccg cggcagtcgc
    16381 tgtggccacc ccgtgctgtc ccgcgccggt ctcagcgatg acccgggact tgcccatccg
    16441 cttggccagc aacgcctggc cgaggacgtt gttgatcttg tgcgagccgg tgtggttgag
    16501 gtcctcgcgc ttgaggatga cccgcgctcc cccgcagtgc tccgcgaacc gcggcacctc
    16561 ggtgatgatc gacgggcgcc cggtgtaggt gcggtgcagc cggtcgagtt caccgatgaa
    16621 ctcggggtcg acggccgcct tgcggcgcac ctcgtcgagt tgttccaggg cggggatgag
    16681 cgcctcgggg acataccgtc ccccgaagtc gccgaatctc ccgtgcggtg tggtcatctc
    16741 ggtccctccg tcgtgctcgc ctgcgtcgtg ctcgcctgcg gctgtcccgg tatgccgccc
    16801 accggcaccc ccgcgaaact cgccaccgcg ccggccgggt cgccgccgat caccagtgcc
    16861 tcaccgacga gcaccgctcg ggcgccctcg gcggcatacc ccttcacgtc ggcgacgccg
    16921 cggatgccgg actcggcgac cgcaaccgcc tcgtcgggga tgagcgggcg cagccgggcg
    16981 aaggcggacg ggtcgacctc gagggtcttg aggttgcgcg cgttgacccc gatgacgcga
    17041 gccccggtcg cgacagcccg gcgggtctcc tcggcgtcgt gcacctcgac cagcgccgtc
    17101 atccccagcc gctgcgcctg ctcgcgcata gcgatcagtt gaatgtcaga gagcgcggcg
    17161 acgatgagca ggatgaggtc ggcgccgtgg gcccgcgcct cgaggagttg atacggctcc
    17221 accatgaagt ccttgcgcag cacggggatc cgcacggtgg cgcgcaccgc gtcaaggtcg
    17281 gccagcgacc cggagaagcg tcgctcctca gtgaggacgg agatcgcggt ggctccgcca
    17341 gcggcatacg cagtcgccaa ggccgccggg tcggggatgt ccgcgagggc gcccttgctg
    17401 gggctggccc gcttgacctc agcgatcacg gcgatgccgg gagcggcccg cagggcggcc
    17461 tctgcgtcga gggcgggagg catcgtcgcg atccgggcct cgaggtcagc caggctcgtg
    17521 cgggcgacgc ggtcagcgag atcgaggcgg acgccagcaa tgatctcgtc gagaacggtg
    17581 ggtgtggcag gcaccgaccc aggctacgtg gtcaggctca gagcggggac aaccatgtca
    17641 tccctgggac gttgcgcagg atccagaacg cgacgatcga cacccacacg acggtgatga
    17701 cccacccggg cgcgagtcgc agcggggtgc cggtgacccg gcggtaggcc cagcggtaga
    17761 acgtccaagc gatgtagatc gaggcgagca ccgcgaaggg gttgcgggcc agagcggtgc
    17821 cgacgtcgag gtgcagcagg gcgtgcagtg cccgtaagga gccacaaccg gggcaccaga
    17881 ggccggtgac ggccaggaac gggcacggcg gatagtggcc gggggcgttg gggtccacgg
    17941 catacaggta tgccgccccc gccgccgcgc ctacggcgat cagggcaggt tgcacaaggc
    18001 cgcgcacccc catgccaaca cggggggacg cggcctcggc gatcgtcaca aggacggttg
    18061 tcagcggacc gtcacactgc cgtttgcggc cgcgaggaag acgaacacga agtagatgat
    18121 gccgacaacg atgccgagga tggccgagat gatcgaccac ttcttcgcct ctgctgcagc
    18181 ggcgtgggcg ccggcctggt cgccctgggc ccacagggtg ttgaccttgt tggccttgag
    18241 gatcgccacg atgcccagag gcaggcagca caggacggtc gcgaggatgg ccaggacgag
    18301 gttgttctgc gggggcgggc tcatcggagc gcccccatag ggcatccctc cggcaggacc
    18361 catgggcggc tgagcgccgt agccaccaga acccggcggc gggggcggcg gagtggaacc
    18421 gtaggacatg acactgcctc tttctcgatc acgacaacaa cgcgcccccc ggacgaggcc
    18481 gcgacaaaga ccctagcgtt caccgcgcac ggtggccaac gaacccgccg aatcgagatg
    18541 ccccgcggac cggcgcatcg agggacggcc ctgggtcagg aaacgccgct gtccacctgg
    18601 ttgcggcccg gcttctggac gccgaaaccg gccatggcca acacctttcc ggtgacgacg
    18661 ccgatgacgg caagcacggc accggcgatg aacaacccgg tcgaggcaac gacgacggcc
    18721 caacacatga tggctgcggc caccgtgagg atggccactc cggtccacgc ggcaacgctc
    18781 tgaccgtggt tgtcgtggtg gtcgtttccg gccatcagtg tcactccttc tacggccgcc
    18841 ggggtcggcg ggcacccgtc acggatttcc tgtgcgccat tgtgtcaggt cccctggcca
    18901 ctgtccgcgc gggtggggtc gtgtccttcg gtgaggtcgt cccagtcgct gcgccgagcc
    18961 ccacgatcgg tcgcgccgtc gacgcctccg cgatcgaacc gtgcggacag gccggcccat
    19021 cgacgcgccg agaaggtcgc cacgatcgag gccacggcgg tgagtccggc gcccgtggcg
    19081 gcgatccacg cccacggccc gagcgcgctc gtcaccggga ccggggaacc cgtgcggccg
    19141 gccaactccg cggcccgccg acccagggcg gccccagccg agccgagcga ggccccgatg
    19201 agccccacga cagcgagcgc agccagcgcc atgaggaccc cggccgcggt ccggatccgc
    19261 ggacctccgg tgagcatcgc gaccagcgct gcaccgacca cgagggccag acccagcgcc
    19321 gcggggacca tctgcgtgcc cgtaatgctc agggccgccc ggcccagcac cgggtcctgg
    19381 ctggacccac tggcccacac ctggcccagc gccgccagca gcaccgccaa cgggccgccg
    19441 acggcgagga cgaccaggcg tttggtcacg gggagaccgc caccgctgcc tgcatccctg
    19501 cggcggcctg gacggcccgc agcacggcgg cagccttgtg ctggcactcc tcgtgctcca
    19561 gttggggcac ggagtcggcg acgatcccgg cacccgcctg gacgtgcgcg acgccgttgc
    19621 ggatgacggc ggtgcggatc gcgatcgcca tgtcgaggtc accggcgaag tcgagatagc
    19681 ccacgactcc cccgtagacc ccccgcttga gccct
//
LOCUS       DAOMZY010000015        19431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_2480_length_19431_cov_6.522967, whole genome shotgun sequence.
ACCESSION   DAOMZY010000015 DAOMZY010000000
VERSION     DAOMZY010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 19431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19431
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2480_length_19431_cov_6.522967"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PLK64_01870"
     CDS             <1..522
                     /locus_tag="PLK64_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV78516.1"
                     /translation="GPRTAPWLIAATLTVYGIGLGLASAQLTGTVLADVPPEQSGAAS
                     ATQSTVRQVGSALGTAVAGTALAAGLGAALPTALATVRGLPADVAAQLITTTTVSAGG
                     VIEATRAHGAHGPLGALGPDVVNALASGFADATRISILAAAVFLAAGLLGAWRVAVVA
                     RDPARSKQVLEPT"
     gene            638..2857
                     /locus_tag="PLK64_01875"
     CDS             638..2857
                     /locus_tag="PLK64_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPV78517.1"
                     /translation="MNLSTRLSSRVGAWLVLVVGLLLAVGILGTLRGSTPPAANRAAP
                     STSESARVDTQLAALPGHDVQNVLLVATRTDGQALSTADLGELGRLSATTTPISGHPV
                     TEPVPSSDKRAAILQVPVRVSADNTENATTITAIRDGIRDTLGPGRGALQVQVTGGPA
                     FGADVAKAFDGANITLLLTTVGIVALLLLLTYRSPILWLVPLAVVGLADQLAAAVTGA
                     VGHAVDLSFDTGIISVLVFGAGTNYALLLISRYREELGRRPDHRDALAHAWRATMPAI
                     VASNLTVVLALGSLVLASIPGTRGLGVAAAIGLLLALATVLTLLPTALAVCGRRLFWP
                     FIPRPGSTSTRRPSIWGVTAAVVDRRPVPVIAATLALLGVLATGLVGTSVGLTQTDRF
                     RSATESAAGLETLAAHFPAGLAQPLLVTTSTAHIPAVAAAIRTVPGATLAPARGTTRP
                     APPEQTEPAQPTGATDLNPDTTQTAGSDSGTAVLPVILEADPGSAEARELVEQVRAAV
                     HAVPGADALVGGAQATDLDARTGQARDLRLVVPLVLAVSLVVLLVLLRSLVAPVLLLS
                     VNALSAVATIGAGSWLGRHLFGWPALDLQVPLLAFLFLVALGIDYTIFLVHRARSEAA
                     TTGTRTGMVTAVTQTGAVITSAGIVLAAVFAALGVLPLVTLGQLGLIVGLGVLLDTFV
                     VRTLLVPAVFAALGERIWWPRHPGAGGRAAAARPVTPPAHVAAAGHRPEQLGSAGHH"
     gene            2939..4144
                     /locus_tag="PLK64_01880"
     CDS             2939..4144
                     /locus_tag="PLK64_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPV78518.1"
                     /translation="MVLTHGEVAADDAPAASPVLRTLVLFEHALFLLLVSVGFLRALL
                     TGATPWPVVAGTFLLIAWYAVGARRLIRPPLLWLTGLTLLWAALALTSPEFTWVGFSL
                     WLLAGHFLPWRWAVAYTLTVLAGVVLLPVRSTGEPNAAAVLGPAIGAAFALAISLAQH
                     QLFREDRERRALLASLLQANAETAALQDQLAAVQHQSGVLAERTRISRDIHDTIAQSF
                     SSILLLARGGRPDPGPALYQIEGAAAAGLAQARHVVSALAPPELDSGSLTSAVGRLLD
                     ELATQTDLQTELRTESDLPELTPATQIALLRTVQGALANVRQHSDAHHVVVTLDRSED
                     TIRLDVVDDGVGFDAADWAGTTPDSYAHGGYGLRSTRARLRELGGGLDIESRPGEGTA
                     LSAHVPSGH"
     gene            4146..4769
                     /locus_tag="PLK64_01885"
     CDS             4146..4769
                     /locus_tag="PLK64_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPV78519.1"
                     /translation="MTTRVLIIDDHPVVRAGIRTLLQQADGVRVVGQAGSGEEALADL
                     ARTRPDVVLCDLRLGAGLDGVATTKAISRTAARPAVLILTTYDNDADIVRAVEAGAVG
                     YLLKDANPTDIIHAIHQAARGETVLSDGMTERLITTMRRQRISLSNREAEVLRLVADG
                     MSNRDIARHLFLSEATVKTHLNHIFSKLGVENRTSAIATARAAGLID"
     gene            complement(5471..8311)
                     /locus_tag="PLK64_01890"
     CDS             complement(5471..8311)
                     /locus_tag="PLK64_01890"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPV78520.1"
                     /translation="MGDESMRAISLRDKGGHFYRADFQVHTPRDTQWDGARPPLAGRE
                     QWAAVFVAAARERGLDAVAISDHHDFTYFPYIRKAAAVEVREDGTPVADAERLVVFPA
                     LELTLSVPCQAIMILDADFPEGRLNDVLKALHFDPVDPSLDALPQTTVLQDSGDINDL
                     HAKLDKHDWLCGRYILLPNVTPSGHKTLLRTSFQAKYRDMTPTGGYLDGSITELDKQK
                     NAGEKRILEGGDAHWGSKRLALFQTSDARKADFSTLGKHSTWVKWSSPTAEALRQACL
                     AQESRLCQVEPALPNVWISRVIVSQSKFLGRVDVALNPQYTALIGGRGTGKSTILDYL
                     RWALCDQPASSTADDEVADPRVRQRRLIEVTLKPLKAHVEVHCVINGITHVVRRYSED
                     GGVQLKVGDAAFEKVREGAIQSLLPIQAYSQKQLSSVAIRIDELLRFVTSPIQRDLEE
                     IERKQHEVAGRLRENYGSLQRHRDLSNEIERSELRVRSLAEQAQALRDGLSGISEADR
                     LVLDSKAAHDRARTAHSAWAQSLGSVHSDLSELVSHLDTSLGDLTKPEGVPADISSEL
                     DASFDASAAALSTLKDKLSALADEFATLLNDGGAVAVPGAALAAKLDEYERTYAAVKA
                     RSSAHEAKLGELATLEQQQKTASELAQRQRKERDTLGKPDEKHRVLRKELTAAWKERS
                     DALAAQCDGLSASSDGMIRATLSVSRGFDAALAKFKALVTGSNVRTNKIEGFFEELAK
                     ESNPAATWELALEELESLMLLEPDADVKSEQTPTLSRLGFAVADQKKIIPKLTPDGWL
                     DLSLTELADFPEFEYRAKEDEYIPFDSASAGQQASALLATLLSQGGTPLIIDQPEDDL
                     DSDTVQEIVAKIWDSKSGRQLIFASHNANLVVNGDADLVLVCAYVNTGDQSAGHIKEQ
                     GAIDVAAIRNQITAVMEGGEKAFRLRKEKYGF"
     gene            complement(8311..11340)
                     /locus_tag="PLK64_01895"
     CDS             complement(8311..11340)
                     /locus_tag="PLK64_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012804179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPV78521.1"
                     /translation="MQEQGRGALDCLMEFKFDARQQYQLDAIGSLVQLFHGQPKDAEV
                     VLTTLRGGGQLNGFEDQTSLDLDLSQEVGAVGNNLVLDEATILQNLQAVQDKNGLQVL
                     GALAGDALDFDIEMETGTGKTYVYLRTVFELAKAYNFTKFVILVPSVAIKEGVVTSLK
                     LMHAHFRELYPGQPFDWTVYDGGSPESVQPFATSTSVQIMVMTIDSIRTKNRGESRLN
                     IHKSRDKLNGLEPIDYLRATRPIVIMDEPQNMESSPSLESIAEMDPTAYLRFSATHRK
                     QRNVVYRLDPVDAHDHGLVKQIVVAEVAQQGSDATPYIKVVEVKRDPWEAKLQLAVRG
                     VDGSLTRRVVKAKQDQELSNLTNNPAYEGWWITELSIEPQFVELNKHGMLQQGEEIGG
                     NSDSIYREMIRETIKEHFRKQTILASKGIKVLSLFFVDKVENFLGDGFNNIDANGQFV
                     QWFDELFLEERAKSPKWQELFPHDPADLRRGYFSVLKAKRGGTDTFQDTSGSTKADDD
                     AYDLIMRDKARLLDQAEPVRFIFSHSALREGWDNPNVFQICTLREMGAEVERRQTIGR
                     GLRLPVDQTGERVADRSIAQLTVIANESYVAFAKSLQTEYEKSGIEIGRVRLQEFSKI
                     ALYEEHIEPNGPEYFGYERSKVLYEALESSGMINDGKVTAAYQPTQLGFDLKQHIPDF
                     FWPYEDIIIEYIDRSSIERYVKQQSKRKARRLNKELFANPEFEEFWRAISARTTYRVS
                     VERDRLIEATVKAIKGAPAIQPLRIQVTRAGVKILRGGTKATETGQRSASLKGAYDLP
                     DIITELQEATSLTRQTIIDILLASGRLPEFIGNPNDFIQMVKGRLQAVLAEIVTEGIQ
                     YEKIGGYIYELRELQKDGQEEKDRFLDQMYKVTKPEDTITKKPRTDFDYVVYESDPER
                     QFAELLDGRDDIKFFMKLPGRFKIDTPVGPYNPDWAIIKHEDGADRIYMIRETKSTED
                     EVKRRPTENAKIKAATKHFAAIGVGDYAVSTPANWRV"
     gene            complement(11304..13262)
                     /locus_tag="PLK64_01900"
     CDS             complement(11304..13262)
                     /locus_tag="PLK64_01900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012804178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPV78522.1"
                     /translation="MTPDDINEVPSTTPDLQTELARQLQELVPEAISDGKVDVAKLTE
                     LLAADVADSSERFGLFWPGKKRALRAAQEPTTATLRPVKDQSKDWDTTKNVFIEGDNL
                     EVLKILQKHYHGKIKMIYIDPPYNTGKDFVYPDNYKEGLETYLEWTKQVNEEGKKVST
                     NSESEGRYHSNWLNMMYPRLKLARNLLTPDGVIVIHIDEHEHANLEKLLNEVFGEGCN
                     LGSIVWDKRNPKGDSTGVAQQHETVMVFCRNREAFTQVADFSRPKPNAQAMLQAAAQA
                     IAANGGVNDLARQAYRDWIKQQPSLTGGEAAYNQIDADGHIYRPVSMAWPNKKKAPDD
                     YFLPLVHPVTGKPCPVPERGWRNPPATMMALLEAGQILFGKDETTQPTRKYLLSDNMY
                     ENVASVLYYAGSDDALLTEMGIPFDTPKPVEVAQRLIQSISRGTDIVLDFFAGSGTAA
                     HAVMALNARDGGSRPWIAVQLPEPTPADSDARAKGFETVAAIALARICAAGDKVRGDL
                     AGQSVDTGFRKYALIDTNFAKWHVASDVDATALEQHLLTLRGSADDNASTEALLSELL
                     LKQGYSLTEHAVDDEIAGLRLQSVGSGLLLAYLDEHTKPSLDQLRAVVDVEPEQLVIL
                     EDAFHGDDELKTNLAQLCKSKGVELWTA"
     gene            complement(13259..16162)
                     /locus_tag="PLK64_01905"
     CDS             complement(13259..16162)
                     /locus_tag="PLK64_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017488020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HPV78523.1"
                     /translation="MTDGQHARVGVGFRPIDGFTDYQAKYYAHELQRSYASDHVGKLA
                     GLLFDAQVEPKPHQIDAALFALQTPFLKGVILADEVGLGKTIEAGIVISQYWAERKRS
                     TLIIAPSSLRQQWQQELYEKFLIPSSLLDAKTKDRLLGVGRSPEVLICSYEFAQRHEL
                     SLLRAWDVVVADEAHRLRNFWNGKAKVANAVAHIVDGAHKTVLLTATPLQNKLEELYG
                     LVSVFDPNYFYSLDAFRERYIKNRESAAFDDLGDRVAQVSKRTLRRDADKYIRFTERL
                     PLTVGFEPSPDEQRLYELVNDYLQREEVYAFSKSTRHLSALIIRKRLGSSTYAIASTL
                     ERVAGRLEREVEEGRRWTNTGSFIVDADLTDEEEEELDEEPKAGFTAIDPRDIEPMKD
                     EVRELREYAALARSITVNEKAVQLSHALDLGFGRLRELGAPQKAIIFTDSTVTQDYIA
                     RSLTEAGRGEGLLLFNGQNDSPQATAIYQKWLERNKGSDVITGVLAADRRKALVDYFR
                     EEGTIMIATEAASEGINLQFCSMVVNYDLPWNPQRVEQRIGRAHRFGQQHNVVVVNFS
                     NKGNLAEQRILELLEDKFHLFKGVFGASDEVLGSIEDGLDFEKTISDILNRCKTAGDI
                     EVAFDQLEKQFEGEISKEMASAKAKVFDNLDPNVQDRLKSYDAQSGEVLNRFEQLLLA
                     VTKYQLDSVATFEGDGRQFELHTAPVVDAKIGRYFFKSQPQEHAHQYRFASPLAEYVV
                     ASSKDADTPPRELTFSLGKSQRASTAIKALHGKSGQLVSKVVTFRMKAGNEDISESYA
                     LAGLLTDDGEWLDHEYVADLMDLACTEVSDPIDMIDESAFEKHLDAQREAFEKEVQAR
                     NARYYDQQEELLYRNTLDRKAESEGLIRDYRQKEKESRKAARQTDDPMEQLRHKKEAR
                     RWAQKAEDEDDRARGDRIKLREEIDKYLDLIEQSLKGKRETEDLFSIRWKVIA"
     gene            complement(16155..16361)
                     /locus_tag="PLK64_01910"
     CDS             complement(16155..16361)
                     /locus_tag="PLK64_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019157620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV78524.1"
                     /translation="MSEPWLSAEEIAVHLGVTKDTVYAWIAHRGMPAHKVGRLWKFQS
                     EEVDAWVRNRGGSDSSDRTGDGRD"
     gene            16703..16933
                     /locus_tag="PLK64_01915"
     CDS             16703..16933
                     /locus_tag="PLK64_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HPV78525.1"
                     /translation="MAERESRIELPPARTGRPAARPRRYAPDELVRFDARIPARLAKQ
                     LYDVALTDGRSVTAVHADLLAAALECCGAAMD"
     gene            17066..18040
                     /locus_tag="PLK64_01920"
     CDS             17066..18040
                     /locus_tag="PLK64_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPV78526.1"
                     /translation="MSSTRPECSSGTENVNRHAEGEDPRAAVFNYVPYAIPDDEWAPV
                     ADFTRESVLLVHPRSERQAIEAMRTVSQFVVWAHREGYPLDRDRMFIPDAVERYIAVA
                     ATHLAVSSRATRRSDLRRFSRQITTKAPWAPDPPQLRQRRTLVPFTPDEIARLWEIAN
                     NQSTKIRRRRFTGYLALGLGAGLHPHEMWKANVGDLETVHDFLCVRVIESRHPRVVPI
                     TAPNDEVLTGFLTEPADAPILAYRPRKWDRSPFHEMLRKADVPPGCPTITPARLRATW
                     MVAHLSAGIAMGGLMQIAGFTSWRPYQPLQQFVAQPDEPTLFAQAVRR"
     gene            18037..>19431
                     /locus_tag="PLK64_01925"
     CDS             18037..>19431
                     /locus_tag="PLK64_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78527.1"
                     /translation="MSRRRRLPIAAAPRPSSDATQANPMTDEQRIARSVAVATSDIAK
                     KICAAAERRTGRPRLIPLSGVLAAYIFHATGEPHTMTTSGVCRSLKKLKPKQRSALGI
                     PKHMQVRYKRMHSGWRAIKRVVEHGVLVEHDHELDVDPDTGEVLDCPQDCPFEEVGLD
                     ELTTLLIQASLPPEFERPPAVAIDGTDIESHTRPHKKRIDTEGRAFWSADPDARWGHR
                     TATDRRPTEHFLGYEAHLATYAPRVGSEPIPQVAAGLAIRPGIRDRARACLNIVDALP
                     RFEDILLDRGYTTAKGSQLAGPLRDREITVTMDLHKTQRVVRPGPEVGTLWVDGSLYS
                     SALPETHRTLEAPTAGMSAAEKSRRRETFDAREPYRFVPHAKRDTTRGTQRFRGPAVD
                     GHPFKVRCPNTPASMRMPHTLPTTTCVPGNPCGCGRTVTVHDSELERDRQHHPWQSTR
                     WALSFNRRTLVEGLI"
BASE COUNT         3124 a         6778 c         5992 g         3537 t
ORIGIN      
        1 ggcccgcgca cggcgccgtg gctcatcgcc gccaccttga cggtgtacgg gatcgggctg
       61 ggcctggcct cggcgcagct gaccggcacc gtcctggccg atgtgccccc agagcagtcc
      121 ggcgccgcgt ccgccacaca aagcacggtg cgccaggtcg gctcggccct gggcaccgcc
      181 gtcgcaggca ccgccctcgc cgctggcctc ggcgccgccc ttccgacggc cttggccacg
      241 gtccgaggac tacccgcgga cgtcgccgcg cagctcatca ccacgacgac ggtcagcgcc
      301 ggcggcgtca tcgaggccac ccgcgcccac ggcgcgcacg gcccgctcgg ggccctgggg
      361 ccggatgtcg tcaacgccct ggcctcggga ttcgccgacg ccacccgcat ctcgattctc
      421 gcggctgcgg tgttcctggc ggccgggttg ctcggagcgt ggcgggtcgc cgtcgtggcc
      481 cgagacccgg cacggagcaa gcaggtactc gagcccacct gaccggcacc gccgaccccg
      541 accgctcgcc agacccgcga cgcggtcccg gtcgacccac ccccacatcc ccccttgccg
      601 ctgcccgacg cggagaccct gacgaaggac gaccaccgtg aacctgtcca cccgcctcag
      661 cagccgcgtc ggcgcctggc tcgtcctggt tgtcgggctg ctcctggccg tcgggatcct
      721 cggcaccctg cgcggctcga cacccccggc cgcgaaccga gccgctccca gcacgtcgga
      781 gtcggcacgc gtcgacacac agctggcggc cctgccgggc cacgacgtgc aaaacgtcct
      841 gctggtcgcc acccgaaccg acggtcaggc cttatccacg gccgacctcg gcgagctcgg
      901 gcgcctgagt gccaccacca cgcccatcag cggccacccg gtcaccgaac ctgtcccctc
      961 cagtgacaag cgggccgcga tcctgcaggt cccggtccgg gtctcggccg acaacaccga
     1021 gaacgccacc accatcaccg cgatccgcga cggcatccgc gacaccttgg gcccagggcg
     1081 gggagccctg caggtccagg tcaccggcgg gccggcgttc ggggccgacg tcgccaaggc
     1141 cttcgacggc gccaacatca ccctgctgct gaccacggtc ggcatcgtcg cgctgctgct
     1201 gctgctgacc taccgctcgc cgatcctgtg gctggtcccg ctggctgtcg tcgggcttgc
     1261 cgaccagctc gcagccgctg tgaccggggc ggtcggccac gccgtcgacc tgtccttcga
     1321 caccggcatc atcagtgtcc tggtgttcgg ggccggcacc aactacgccc tgctgctgat
     1381 ctcccgctac cgcgaggagc tgggccgccg ccccgaccac cgggacgcgc tggctcacgc
     1441 ctggcgggcc accatgccag ccatcgtggc cagcaacctg accgtggtcc tcgccctcgg
     1501 ctcgctcgtg ctggccagca tcccgggcac ccgcggcctg ggggtcgccg cggcgatcgg
     1561 gctgctgctc gccctggcca ccgtcctgac gctgctaccg accgccctgg cggtctgtgg
     1621 gcggcgcctg ttctggccgt tcatcccccg ccccggcagc accagcactc ggcgacccag
     1681 catctggggt gtcaccgctg cggtcgtgga ccgccgaccc gtcccggtca tcgccgccac
     1741 gctcgccctg ctcggcgtgc tcgccaccgg cctcgtcggc acctccgtcg gcctgaccca
     1801 gaccgacagg ttccgcagcg ccaccgagtc cgccgccggg ctggagaccc tggccgcaca
     1861 cttcccggcc ggcctcgccc agcccctgct ggtcaccacc tcaaccgccc acatccccgc
     1921 ggtcgccgca gccatccgga ccgttcccgg cgctaccctc gcacccgcca gaggtaccac
     1981 ccgacccgcg ccaccagagc agaccgagcc tgcgcaaccc accggagcca cagacctcaa
     2041 cccggacacc acccagacgg ccggctctga ctccgggact gccgtcctcc ccgtgatcct
     2101 ggaggccgac cccgggtcgg cagaagccag ggagctcgtc gagcaggtcc gggccgcggt
     2161 tcacgccgtc cccggggccg acgccctcgt cggcggagcc caggcgacgg acctggacgc
     2221 ccgcaccggc caggcccgcg acctgcgcct cgtcgtgcct ctcgtcctcg cggtcagcct
     2281 cgtcgtgctg ctcgtgctcc tgcgctccct cgtcgccccg gtgctgctgc tgagcgtcaa
     2341 cgccctgtcc gccgtcgcca ccatcggtgc tggctcgtgg ttgggccgac acctgttcgg
     2401 ctggccggcg ctcgacctgc aggtcccgct cttggccttc ttgttcctcg tcgccctcgg
     2461 catcgactac accatcttcc tggtccaccg ggcccgctcc gaggcggcca cgaccggcac
     2521 ccgaaccggg atggtcaccg ctgtcaccca gaccggcgcg gtcatcacca gtgccggcat
     2581 cgtcctcgcc gccgtcttcg ccgccctcgg cgtgctgcct ttggtcaccc tcggccagct
     2641 cgggctgatc gtcgggctcg gggtcctgct cgacaccttc gtggtccgca ccctgctcgt
     2701 gccagccgtc ttcgccgcac tcggcgaacg gatctggtgg ccccggcacc ccggcgccgg
     2761 cggtcgagca gcggccgcgc gcccggtaac gccccccgcc cacgtcgccg ctgccggcca
     2821 ccggcccgag cagctcgggt cagccggaca ccactaacga cgacgtcctt cgacccaccc
     2881 gcggaccacc actgggatga tgtagacgcc caccagcaac ccccggaagg aggcacacat
     2941 ggtgctcacg cacggcgagg ttgctgccga cgacgcgcca gccgcgagcc cggtcctgcg
     3001 cacgctcgtg ctgttcgaac acgccttgtt cctcctgctg gtcagcgtcg ggttcctccg
     3061 cgcgctgctc accggcgcca ccccctggcc ggtcgtcgcc ggcaccttcc tgctcatcgc
     3121 ctggtacgcc gtcggcgctc gccgcctgat acgccccccg ctgctctggc tcaccggctt
     3181 gaccctgctg tgggctgccc tggcactgac ctcaccggag ttcacctggg tcggtttcag
     3241 cctctggttg cttgctggcc acttccttcc ctggcggtgg gcggtggcgt acaccctgac
     3301 cgtgctcgcc ggggtcgtcc tcctccccgt ccgcagcacc ggagagccga acgctgcggc
     3361 cgttctcgga cccgccatcg gcgccgcctt cgcgctggcc atctccttgg cccagcatca
     3421 gctgttccga gaggaccgcg aacggcgcgc cctgctggcc tcactcctgc aggccaacgc
     3481 cgagactgcc gccctgcagg accagctcgc cgccgtgcaa caccagtccg gggtcctcgc
     3541 cgaacggacc cgcatctccc gcgacatcca cgacaccatc gcgcagagct tctcctccat
     3601 cctcctcctc gcgcgaggtg gacgccccga ccccggccca gccctgtacc agatcgaagg
     3661 cgccgccgcc gccggactgg ctcaagcccg gcacgtcgtc agcgccctgg ccccaccgga
     3721 gctggactcc ggcagcctca cctcagcagt ggggcggctc ctcgacgagc tcgccaccca
     3781 gaccgacctg cagaccgagc tgcgcaccga aagcgacctg cccgaactca ctcccgccac
     3841 ccagatcgca ctgctgcgca ccgtccaagg cgccctggcc aacgtccggc aacactccga
     3901 cgcccaccac gtcgtggtca ccctcgatcg cagcgaggac accatccgac tcgacgtcgt
     3961 cgacgacggg gtcgggttcg acgccgccga ctgggctggc acgacacccg actcctacgc
     4021 ccacggcggc tacggtctac gctccacccg cgcccgactc cgcgagctcg gcggaggatt
     4081 ggacatcgag tcacgccccg gcgagggcac cgccctgtcc gctcacgtgc cctcaggcca
     4141 ctgacatgac aacccgcgtc ctgatcatcg acgaccaccc cgtcgtacgc gccggcatcc
     4201 ggacgctgct ccagcaagcc gatggagtcc gcgtggtcgg acaagccgga tccggcgagg
     4261 aagccctcgc cgacctcgcc cgcacccgcc cggacgtggt cttgtgcgac ctacgcctcg
     4321 gggccgggct cgacggagtc gccaccacca aagccatctc gcggaccgcg gcacgaccgg
     4381 ccgtactcat cctcaccacc tacgacaacg acgccgacat cgtccgggcc gtcgaagccg
     4441 gcgcagtcgg ctatctcctg aaagacgcca acccgaccga catcatccac gccatccacc
     4501 aagcggcccg aggcgagacc gtcctgtccg acggcatgac cgaacggctc atcaccacca
     4561 tgcggcgtca acgcatctcg ctcagcaacc gcgaagcaga ggtcctcagg ctcgtcgccg
     4621 acggcatgtc caaccgcgac atcgcccgcc acctcttcct cagcgaggca accgtcaaaa
     4681 cccacctgaa ccacatcttc agcaagctcg gcgtcgagaa ccgcacctcg gccatagcca
     4741 cggcccgagc cgccggcctc atcgactgat tgcggccctc cgccctgctt ggccaaccat
     4801 cccggggtgt ccgccgcccc gtcctcgccg tcgtgcttcc acgccttgtt cacccgcacg
     4861 gtgccgcggc gcagatcgac gtccccgacc catagggccc ccggccggct gaagagcagc
     4921 gagccgcgga cccgtgtccg ggcgagcacc ccgatctgac cgagatagcg cttccgttgc
     4981 ccaggagaga ggccacgatc tgccgcatga acccctcgcc cctcacccag ccgtccggcc
     5041 agtgctggcc cgccgcgtcg accatcttct ggaacccgtc cgccctcgcc aggttttgcg
     5101 catcactgcc cgcgctgaac gtctccgccc ggtaggcccc ggcccgggct cccccgagac
     5161 gccacaccac gcccgcagac accccgccga cggccccggg tccgcttgtc gagccacgcc
     5221 atcacaccgc cccttccaag tgcgctggtg ctaccaccgt gctacctcag aaggccagag
     5281 tgcgcccgac aacgacgaaa ccccaggtca gacctggggt tcatcagtgg gtagttcccg
     5341 aaagtcgctg acctgctgcg acggttaggg aatcccgcgt gtaggcacaa gtagctgagc
     5401 tcaccaggca cagccaagcc gatgtacttc tcagcctgcc ctgttcgaag agactgcgtc
     5461 gcgccccggg tcagaaaccg tacttctcct tgcggagacg aaacgccttt tccccgcctt
     5521 ccatcacagc ggtgatctgg ttccgaattg ccgccacgtc gatagcaccc tgctctttga
     5581 tgtggcctgc ggactgatca ccagtgttga catacgcgca gaccagcacg aggtctgcgt
     5641 cgccgttgac gaccaagttg gcgttgtgac ttgcaaagat gagctgtcga ccgctcttcg
     5701 agtcccagat cttggccacg atctcttgaa cggtgtcact gtcaaggtcg tcctccggct
     5761 ggtcgatgat cagcggggta cctccctgcg acaacagcgt ggcgagaagg gctgaggcct
     5821 gctggccggc ggatgccgaa tcgaacggga tgtactcgtc ctccttagcc cggtactcaa
     5881 actccgggaa gtcagcgagt tcggtgagtg acaggtcaag ccagccgtcg ggggtgagtt
     5941 tgggaatgat cttcttctga tccgccacgg cgaacccgag ccgcgacagc gtaggagtct
     6001 gctcggactt cacgtcggcg tccggctcca ggagcattag cgattcgagc tcctcaagag
     6061 ccagctccca agtcgcagcc gggttcgact ccttcgccag ttcctcgaag aatccctcga
     6121 tcttgttggt gcgtacgttc gatccagtaa cgagagcctt gaacttggcg agcgctgcgt
     6181 cgaagccgcg gctcacggaa agggtcgcgc ggatcatgcc gtccgacgag gccgaaagcc
     6241 cgtcgcactg tgcagccagc gcgtcggagc gttccttcca agccgccgtc aactccttcc
     6301 gcagcactcg gtgcttctca tccggcttgc cgagcgtgtc acgttccttg cgttgccgct
     6361 gtgccagctc agacgcggtc ttctgctgct gctccagggt cgccagttca cccagcttcg
     6421 cctcgtgcgc ggatgatcgc gccttcaccg cagcgtaggt cctctcgtac tcgtccagct
     6481 tggccgcaag cgccgccccg gggaccgcta ccgcgcctcc gtcgttcagg agggtcgcaa
     6541 actcatcagc aagggccgag agcttgtcct tgagggtcga caacgccgcc gccgaggcat
     6601 caaacgaagc atcaagttcg ctcgagatat cggcgggaac gccctccggc ttcgtcaagt
     6661 cgccgagcga ggtatcgagg tgggagacca attcggagag gtccgagtgc acgctgccca
     6721 ggctctgggc ccaagccgag tgagccgtgc gcgcgcgatc gtgcgcagcc ttactgtcca
     6781 gcacaaggcg atcggcttcg gagatgccgg acagtccgtc gcgtagcgct tgagcctgct
     6841 cggcgagcga tcggactcgc agttccgacc gctcgatctc attcgacagg tcgcggtgcc
     6901 tctgcagcga gccgtagttc tctcgcaggc gcccagcgac ctcatgctgc tttcgctcga
     6961 tctcctcgag atcgcgctga atcggtgacg tcacgaaccg cagcagttca tcgatccgga
     7021 tcgccacgct gctgagctgc ttctggctgt acgcctggat cggcaacagg ctctgaatcg
     7081 ccccctcccg gaccttctcg aaggcggcgt cgccaacttt gagttgaact ccaccgtctt
     7141 ccgaatatcg acgaacgacg tgagtgatcc cattgatgac gcaatgaacc tcgacatgag
     7201 ccttgagtgg cttgagcgtc acctcgatca agcggcgctg ccggactcgg ggatctgcga
     7261 cctcgtcgtc cgccgtcgag cttgccggct ggtcacacag ggcccatcga aggtagtcca
     7321 agatggtcga cttgccggta ccacgcccac cgatcagtgc ggtgtactgc ggattcagag
     7381 cgacatcgac ccggccaagg aacttgctct gggagactat gacgcgcgaa atccaaacat
     7441 tcgggagagc tggttcgacc tggcaaagcc gagactcttg cgcgagacat gcctgtcgga
     7501 gggcttccgc cgtcggtgac gaccacttca cccacgtcga gtgtttccca agcgtcgaaa
     7561 agtcagcctt ccgggcatca gacgtctgga agagcgcgag ccgcttcgaa ccccaatgcg
     7621 catcgccgcc ttcgaggatg cgcttctccc ctgcgttctt ctgcttgtcg agctcggtga
     7681 ttgacccgtc gaggtagccg ccagtcgggg tcatgtctcg gtacttcgcc tggaacgagg
     7741 tgcgcagcaa cgtcttgtgg ccggatggcg tgacgttagg cagaaggatg taccgtccac
     7801 acaaccagtc gtgcttgtcg agtttggcat gtagatcgtt gatgtccccc gaatcctgaa
     7861 gaacggtagt ttgcgggagg gcatcaagcg atgggtcaac cgggtcgaag tgaagcgcct
     7921 tgagaacgtc gttgaggcgg ccctccggga agtccgcatc gaggatcatg atcgcctgac
     7981 atggaacact aagcgtgagt tcaagcgccg ggaagacgac caggcgttcc gcgtcagcaa
     8041 ctggcgtgcc gtcttcgcgc acctccaccg cagccgcctt gcggatgtag gggaagtacg
     8101 tgaagtcgtg gtgatccgag atcgccacgg cgtcgaggcc acgctccctc gccgcggcca
     8161 cgaagaccgc ggcccactgc tcgcgcccag ccaagggcgg ccgcgcgcca tcccactgcg
     8221 tatcccgcgg cgtgtggacc tgaaagtccg cgcggtagaa gtgaccgccc ttgtccctaa
     8281 gcgagatcgc ccgcatggac tcgtcgccca tcacaccctc cagttggcag gcgtggagac
     8341 ggcgtagtcg ccgaccccga tcgcggcgaa gtgcttggtc gcggccttga tcttggcgtt
     8401 ctcggtcggg cggcgcttga cctcgtcttc agtgctcttc gtctcgcgga tcatgtagat
     8461 ccggtcggcg ccatcctcgt gcttgatgat cgcccagtcc gggttgtacg ggccgacggg
     8521 agtgtcgatc ttgaacctcc ccggcagttt catgaagaac ttgatgtcat cacgcccgtc
     8581 gagaagctcg gcgaactggc gctcgggatc agactcgtaa accacgtaat caaagtcagt
     8641 ccgaggcttc ttggtgatcg tgtcctcagg cttcgtgacc ttgtacatct ggtcgaggaa
     8701 gcggtccttc tcctcctggc cgtccttctg cagctcgcgc agctcgtaga tatagccgcc
     8761 gatcttttcg tactggatgc cctcggtgac gatctcggcg agcactgcct gcaggcgacc
     8821 cttgaccatc tggatgaagt cattggggtt cccgatgaac tcgggcaggc gcccgctggc
     8881 caggaggatg tcgatgatcg tctgtcgcgt tagcgacgtg gcttcctgca gttcggtgat
     8941 gatgtcgggc aggtcgtagg cgcccttgag ggaagcggac cgttggccgg tctcggtcgc
     9001 cttggtgccg ccacgaagaa tcttgactcc ggcccgggtt acttggatgc ggaggggctg
     9061 gatggccggc gctcccttga tcgccttcac agtcgcctca atgagccggt cacgctcgac
     9121 cgaaacccgg tacgtcgtgc gagccgagat ggcgcgccag aactcctcga actccgggtt
     9181 cgcgaagagc tccttgttga gcctgcgtgc cttccgcttg gactgctgct tcacgtagcg
     9241 ctcgatgctg gagcggtcta tgtactcgat gattatgtct tcgtacggcc agaagaagtc
     9301 cgggatgtgc tgtttgaggt cgaaccccag ctgggtcggc tggtaggccg cggtgacctt
     9361 gccgtcattg atcatccccg aggactccag cgcctcgtag aggactttgg aacgctcgta
     9421 gccgaagtac tccgggccgt tgggctcgat gtgctcttcg tagagcgcga tcttggagaa
     9481 ctcctgcagc cgaacacggc cgatttcgat gccggacttt tcgtattcgg tctggagcga
     9541 cttcgcgaac gcgacgtagg actcgtttgc gatcacggtg agctgagcga tgcttctgtc
     9601 cgcgacgcgc tctccggttt ggtcgaccgg gagacggagg cctcgaccga tggtctggcg
     9661 gcgctcgacc tcggcaccca tttcgcgcag ggtgcaaatc tggaagacgt tcgggttgtc
     9721 ccacccctct cgcagtgcgg agtgactgaa gatgaaacgg acgggctctg cctggtcgag
     9781 cagccgagcc ttatcgcgca tgatcaggtc ataggcatcg tcgtcggcct tggtgctgcc
     9841 ggaggtgtcc tggaaggtgt cggtcccacc gcgcttggcc ttgagcaccg agaagtagcc
     9901 ccgtcggagg tccgcgggat cgtgcgggaa gagctcctgc cacttcggtg acttggcgcg
     9961 ctcctcgagg aacagctcat cgaaccactg cacgaactgg ccgttggcgt cgatgttgtt
    10021 gaagccgtcg ccgaggaagt tctcgacctt gtcgacgaag aacaggctca gcaccttgat
    10081 gcctttcgac gccagaatcg tctgtttgcg gaagtgctcc ttgatggtct cgcggatcat
    10141 ctcgcgatag atcgagtcgg agttgccgcc gatctcctcg ccctgctgga gcatgccgtg
    10201 cttgttgagc tctacgaact gcggctcaat cgacagttcg gtgatccacc aaccctcgta
    10261 ggcggggttg ttggtcaggt tggacagctc ctggtcttgc ttggccttca cgacccggcg
    10321 agttagggag ccgtcgacgc cgcggaccgc cagttggagt ttggcctccc aagggtcgcg
    10381 cttcacctcc acgaccttga tgtacggcgt ggcatccgag ccttgctggg ccacctctgc
    10441 gacgacgatc tgcttgacga gcccgtgatc gtgcgcgtcg accgggtcaa gacggtagac
    10501 gacgttgcgc tgcttgcggt gagtcgcgga gaacctcaga tatgccgtcg ggtccatctc
    10561 cgcgattgac tccagcgacg gactggattc catgttctgc ggctcgtcca tgatgacgat
    10621 agggcgtgtc gctcgcaggt aatcgatggg ctccaagccg ttgagcttgt ctcggctctt
    10681 gtgaatgttc agacgggact caccgcgatt cttcgtacga attgagtcga tggtcatcac
    10741 catgatctga acgctcgtgg acgtggcgaa cggctgcacg gactcagggg aaccgccgtc
    10801 gtagacggtc caatcgaagg gctgacccgg gtacaactcc cgaaagtgtg cgtgcatgag
    10861 cttgaggctc gttacaacgc cttccttgat cgccacactc ggcacaagga tgacgaactt
    10921 agtgaagttg taggctttgg ctagctcaaa gaccgtgcgc agatagacgt acgtcttacc
    10981 cgtgccggtc tccatctcaa tgtcgaagtc gagagcgtcc ccagcgaggg cgccgagaac
    11041 ctgcagaccg ttcttgtctt ggaccgcctg caggttctgc aggatggtcg cctcgtcgag
    11101 gacgaggttg ttgccgaccg ctccgacctc ctgcgaaagg tcgagatcca ggctggtctg
    11161 gtcctcgaat ccgttcagct ggccgccgcc gcgcagcgtc gtcagcacca cttccgcgtc
    11221 cttgggctga ccgtggaaga gctgaaccaa ggatccgatg gcgtcgagct gatactgctg
    11281 tctggcgtcg aatttgaatt ccatcaggca gtccagagct ccacgccctt gctcttgcaa
    11341 agctgggcga ggttggtctt cagttcgtcg tcaccgtgga aggcgtcctc aaggatcaca
    11401 agctgctcgg gctccacatc tacaacagcg cgaagttgat cgagcgaagg cttcgtgtgc
    11461 tcgtcgaggt acgcgagcag cagaccgctc ccaacggatt gcaacctaag tccggcaatt
    11521 tcgtcatcga cggcgtgctc cgtgagtgag tagccctgct taaggagtag ttcactcagc
    11581 aaagcctcgg ttgaagcgtt gtcgtcggcg ctaccccgca acgtaagaag gtgctgttct
    11641 agagccgtgg catctacatc actggccaca tgccactttg cgaagttggt gtcaatcagg
    11701 gcgtacttcc gaaacccggt gtcgaccgac tgcccggcga ggtcgccccg gaccttatcg
    11761 cctgctgcgc agatcctcgc cagagcgatg gcggccacgg tctcgaaacc ctttgcacga
    11821 gcatcggaat cagccggtgt aggttccgga agctgaactg caatccacgg gcgacttccg
    11881 ccgtcacgtg cgttcagtgc catcacggcg tgcgccgcgg ttcctgaccc agcaaagaag
    11941 tcgaggacga tgtcagttcc ccgcgaaatt gactgaatca gtcgctgggc aacctcgacc
    12001 ggcttgggcg tgtcgaatgg gattcccatc tcagtcagca gcgcgtcgtc gctaccggcg
    12061 tagtacagga cagatgccac attctcgtac atgttgtctg acaggagata cttcctggtc
    12121 ggctgcgttg tctcgtcctt tccgaagagg atctgacctg cctccaggag cgccatcatc
    12181 gtggcaggcg gatttcgcca accgcgctcc ggcaccgggc agggcttccc tgtgaccggg
    12241 tgaacgagtg gcaggaaata gtcgtctggg gccttcttct tgttaggcca agccatggag
    12301 acggggcggt agatgtgtcc gtccgcgtcg atctgattgt aggcggcctc accgcccgta
    12361 agactgggct gctgcttgat ccaatcgcgg tatgcctggc gagcgaggtc attaactcca
    12421 ccattcgctg caattgcttg agcagcagct tgaagcatcg cctgcgcgtt cggctttgga
    12481 cgcgagaagt ccgcgacctg tgtaaacgct tctcggttcc gacagaacac catcaccgtc
    12541 tcgtgctgct gggctacgcc agtcgagtcg cccttggggt tgcgcttgtc ccagacaatc
    12601 gacccgaggt tgcatccctc cccgaacacc tcgttcagca gcttctcgag attcgcatgc
    12661 tcgtgttcgt caatatggat gacgatcaca ccgtctggcg taagcaggtt ccgcgcgagc
    12721 ttcagccgcg ggtacatcat gttgagccag ttggagtggt accggccttc ggactcactg
    12781 ttggttgaga ccttcttgcc ctcttcgttg acttgcttgg tccactcgag gtacgtctcc
    12841 aatccctcct tgtagttgtc ggggtaaacg aagtccttgc ccgtgttgta cggcggatcg
    12901 atgtaaatca tcttgatctt gccgtggtaa tgcttctgaa gaatcttgag cacttcgagg
    12961 ttgtcgccct cgatgaacac gttcttcgtc gtgtcccagt ccttgctctg atccttgacc
    13021 gggcgcagag tcgcagtggt tggctcctgg gcggcgcgga gtgcgcgctt cttacccggc
    13081 cagaacagtc cgaagcgctc gctgctgtcg gcgacgtccg ccgcgaggag ctcggtcagc
    13141 ttggcgacgt cgaccttgcc gtccgagatc gcctcgggca ccaactcctg cagctggcgc
    13201 gccagctcgg tctgcaggtc aggggtggtg ctcgggactt cgttgatgtc gtcgggggtc
    13261 atgcgatcac tttccatcgg atgctgaaca ggtcttcggt ctcgcgcttg cccttgagcg
    13321 actgctcgat caggtcgaga tatttgtcga tctcttcccg gagcttgatc cggtcgccgc
    13381 gtgcgcggtc gtcctcgtct tcggccttct gcgcccagcg cctggcttcc ttcttgtgtc
    13441 gcagctgctc catcgggtcg tcggtctgtc gcgccgcctt ccggctctcc ttctccttct
    13501 ggcggtagtc gcggatcagc ccttcggact cggccttgcg gtcgagcgtg ttccggtaga
    13561 gcagctcctc ctgctggtcg tagtagcgag cgttgcgtgc ctgcacttcc ttctcgaacg
    13621 cctctcgctg ggcgtccaga tgcttctcga aggccgactc gtcgatcatg tcaatcgggt
    13681 ccgacacttc tgtacaggcg aggtccatca agtcagcgac gtactcgtgg tcgagccact
    13741 caccgtcgtc cgtcaacagg cccgcgagcg cgtacgactc ggagatgtcc tcgttgccgg
    13801 ccttcatacg gaacgtgaca accttgctca cgagctgccc gctcttgcca tgcagcgcct
    13861 taatcgccgt gctggcccgc tgggacttgc cgaggctgaa cgtcagctca cggggaggcg
    13921 tgtccgcgtc cttcgagctg gcgacgacgt actcggctag cgggctggcg aagcggtact
    13981 ggtgggcatg ttcctgcggc tgcgacttaa agaagtagcg tccgatcttg gcatcgacga
    14041 ccggcgcagt gtggagctcg aactggcggc cgtctccctc gaaggtggcg acgctgtcga
    14101 gttggtactt ggtgaccgcc agcaggagct gctcgaaacg gttgagcacc tcgccagact
    14161 gggcgtcgta cgacttgagg cggtcctgga cgttggggtc gaggttgtcg aagaccttgg
    14221 ccttggcgct ggccatctcc ttggagatct cgccctcgaa ctgcttttcc agttgatcga
    14281 acgcgacctc gatgtcgccc gcggtcttgc agcggttgag gatgtcgctg atggtcttct
    14341 cgaagtcgag gccgtcctcg atggagccga gcacctcgtc gctggcaccg aagacgccct
    14401 tgaagaggtg gaacttgtct tccagcagct cgaggatgcg ctgctcggcg aggttgccct
    14461 tgttggagaa gttcacgacg acgacgttgt gctgctggcc gaagcggtgg gcgcgtccga
    14521 tgcgctgctc aacgcgctgc ggattccagg gcaggtcgta gttcacgacc atggagcaga
    14581 actgcaggtt gatgccctcc gaggctgcct cggtggcgat catgatcgtg ccctcttcgc
    14641 ggaagtagtc cacgagagcc ttgcggcggt cggctgccag gacgcccgtg atgacgtcgc
    14701 ttcccttgtt gcgttcgagc cacttctgat aaatcgccgt ggcctgtgga gagtcgttct
    14761 ggccgttgaa caggagcagt ccctcgcccc gtccggcctc ggtgagcgag cgtgcgatgt
    14821 agtcctgggt caccgtggag tcggtgaaga tgatggcctt ctgaggagct cccagctcgc
    14881 gcaagcgccc gaagccgagg tcaagggcgt gactgagctg gactgccttt tcgttgactg
    14941 tgatcgaccg ggccagcgcg gcgtactcac gcagctcacg gacctcgtcc ttcatgggct
    15001 caatgtcgcg cgggtcgatg gccgtgaacc cggccttcgg ctcctcgtcg agctcctctt
    15061 cttcctcgtc ggtgaggtcg gcgtcaacga tgaagctgcc ggtgttcgtc cagcgcctgc
    15121 cctcctcgac ttctcgctcc agtcgacccg cgacgcgctc aagcgtgctg gcgatggcgt
    15181 aggtcgagga gccgagacgc ttgcggatga tcagggcgct gaggtggcgg gtggacttcg
    15241 agaaggcgta gacctcctcg cgctggaggt agtcgttgac cagctcgtag aggcgctgct
    15301 cgtcggggga cggctcgaat cccacagtga gtgggagacg ctcggtgaac cggatgtact
    15361 tgtcggcgtc gcggcgcagc gttcgcttgc tgacctgcgc gacgcggtcg ccgaggtcgt
    15421 cgaaggctgc cgactcccgg ttcttgatgt accgctcgcg gaaggcgtcc agcgagtaga
    15481 agtagttggg gtcgaagacc gagaccaggc cgtaaagctc ctcgagtttg ttctgcagcg
    15541 gcgtggcggt gagcaggacg gtcttgtgcg cgccatcgac gatgtgggcg accgcgttgg
    15601 cgaccttggc cttgccgttc cagaagttgc gcaaacggtg agcctcgtcg gcgacgacga
    15661 cgtcccacgc gcgtaggagc gacagctcgt ggcgctgggc gaactcgtac gagcagatca
    15721 ggacctcggg cgatcggccc acgccgagca ggcggtcctt ggtcttggcg tcgaggagcg
    15781 agctcgggat caggaacttc tcgtagagtt cctgctgcca ctgctggcga aggctggacg
    15841 gcgcgatgat cagggtgctg cgctttcgct cagcccagta ctggctgatg acgatgcccg
    15901 cctcgatcgt ctttccgagg ccgacctcgt cagcgaggat cacgcccttg aggaacgggg
    15961 tctggagcgc gaagagcgcc gcgtcaatct ggtgcggctt gggctcgacc tgagcgtcga
    16021 acaggaggcc agcgagcttg ccgacgtgat cgctggcgta gctgcgctgg agttcgtgag
    16081 cgtagtactt cgcctgatag tcggtgaagc cgtcgatcgg tcgaaaaccc acacctacgc
    16141 gagcgtgttg cccgtcagtc acgcccgtca cccgttcggt ccgaactgtc gctaccgccg
    16201 cgattgcgta cccacgcgtc cacttcctcg ctctggaact tccacaggcg acccaccttg
    16261 tgcgcgggca tgccgcgatg ggcgatccac gcgtacaccg tgtccttggt gactccaagg
    16321 tggaccgcga tctcttcggc gctcagccat ggctccgaca cgaagctcct cactccggtt
    16381 aacaccgacc ctaaccgggt gtgaccgggt ttgcgcggcg atggccgaaa cctacgggtc
    16441 atgcgcagct cgtggcccga atgtgcagca agttgctgca cattcggcag caggttgctg
    16501 ccggactgcg gttgggcatc cgcgggcgcg tcgtcgtagc gaaacagcag cgcggatccc
    16561 tactctggac ctagccacgc gctcaggcgc gggggtgagc cactcgtccg ggcgactgct
    16621 cggtggcgcc agagaactga cccgcctctg ttgttgacgg ccaccaaccg tctggcgaca
    16681 gagcgacggg ttggagaggc gagtggcaga gcgagaaagc cggatcgaac ttccgcctgc
    16741 gcgcaccggc cgaccagccg cgcgtcctcg ccgctacgcc cctgatgagc tcgtgcgctt
    16801 cgacgcccgg atccctgccc ggctcgcgaa gcagctctac gacgtcgccc tgaccgacgg
    16861 ccggtccgtg acggccgtcc atgccgacct cctggccgcc gctctggagt gttgcggagc
    16921 cgcgatggac tgagcctctc ggcgcaggtc gccgacatgc gaaaggtcca gagcgccaac
    16981 tccggacctc gtcgcaaatc gcttcagcga gaagtgagct cgctgccttc atccacctgc
    17041 gcaagcaccc tgagggggta cttctatgtc ttcaacgcgt ccagagtgct cttctggaac
    17101 tgagaacgtc aaccgccacg ccgaaggcga ggacccacgc gccgcggtct tcaactacgt
    17161 gccgtacgcc atccccgacg acgagtgggc gcccgtcgcc gacttcaccc gtgagtccgt
    17221 gctcctcgtc cacccccgga gcgaacgaca ggccatcgag gcgatgcgca cggtctcgca
    17281 gttcgtggtc tgggcacacc gcgagggcta cccgctggat cgggaccgga tgttcatccc
    17341 cgacgccgtc gagcgctaca tagcggtcgc cgccacccac ctcgccgtgt cttcgcgcgc
    17401 gacccgccgc tcggacctgc ggcggttctc ccgacagatc acgacgaagg caccgtgggc
    17461 acccgacccg ccccagctga ggcagcgcag gacgctcgtg ccgttcactc cggacgagat
    17521 cgcgcgcctg tgggagatcg ccaacaacca aagcacgaag atccgccgtc gccgcttcac
    17581 cggctacctc gcgctcggtc tcggcgctgg actgcacccg cacgagatgt ggaaggccaa
    17641 cgtcggcgac ctcgagaccg tccacgactt cctctgcgtg cgcgtcatcg agagccgaca
    17701 tccccgagtc gtgccgatca ccgcacccaa cgacgaggtc ctcaccggct tcctgaccga
    17761 gccggcagac gcgccgatcc tggcgtaccg ccccaggaag tgggaccgct caccgttcca
    17821 cgagatgttg cgcaaggctg acgtcccgcc cggctgcccg acgatcaccc cggcccgtct
    17881 gcgggccacg tggatggtcg cgcacctgtc ggctggcatc gcgatgggtg ggctgatgca
    17941 gatcgccgga ttcacctcgt ggcgtcccta ccagccactc caacagttcg tggcgcagcc
    18001 tgacgagccc accctgttcg cccaggcggt ccgccggtga gccgacgtcg gcggctgccg
    18061 attgcggctg ctccgcggcc gagttcggac gccacgcagg ccaacccgat gacggacgag
    18121 cagcgcatcg cgcgctcggt tgccgtcgcc accagcgaca tcgccaagaa gatctgcgcg
    18181 gctgcggaac gccggaccgg acggccacgg ctcattcctt tgtcgggggt ccttgcggcg
    18241 tacatcttcc acgccaccgg ggagccgcac acgatgacca cgtccggcgt gtgccgatcg
    18301 ttgaagaagc tcaagccgaa gcaacgctcg gcgctcggga tcccgaagca catgcaggtc
    18361 aggtacaagc ggatgcactc cggctggcgg gccatcaaac gcgtcgtcga gcacggtgtc
    18421 ctggtcgagc acgaccatga gctcgacgtt gacccggaca cgggcgaggt tctggactgc
    18481 ccgcaagatt gccccttcga agaagtcggc ctggacgagc tcacgacgct gctcatccag
    18541 gccagcctcc cgccggagtt cgagcgaccg ccggcggtgg ccatcgacgg caccgacatc
    18601 gagagccata cccgaccgca caagaagcgc attgataccg agggccgcgc cttctggtca
    18661 gccgaccccg acgcacgctg gggccaccgc acggccaccg accgccgccc taccgaacac
    18721 ttcctcggat acgaagcaca cctcgccacg tacgcgccgc gcgtcggctc cgaacccatc
    18781 ccgcaagtcg ccgccggcct cgccatccga ccggggatcc gcgaccgcgc ccgagcctgc
    18841 ctgaacatcg tcgacgccct cccgcgattc gaggacatcc tgctcgaccg cggctacacc
    18901 acggccaagg gcagccagct cgccggcccc cttcgggacc gcgagatcac cgtgaccatg
    18961 gacctgcaca agacccagcg ggtggtccgc cccggccccg aggttgggac gctctgggtc
    19021 gacggcagcc tctactccag tgccctgccc gagacccacc gcacgctcga ggcacccacc
    19081 gctggaatga gcgccgccga gaagtcgcga cgccgtgaaa ccttcgacgc gcgcgagccc
    19141 taccggttcg tgccgcatgc caaacgcgac acgacccgcg gtacgcagcg attccgcggc
    19201 cccgcggtcg acgggcaccc cttcaaggtc cgttgcccca acacacccgc gtcgatgcgg
    19261 atgccgcaca ccctcccgac cacgacctgc gtaccgggca atccctgcgg atgcggcagg
    19321 accgtcaccg tccacgacag cgagctcgag cgagaccggc agcaccaccc ctggcagtcc
    19381 acccgctggg cgctctcctt caaccggcgc accctcgtcg agggtcttat c
//
LOCUS       DAOMZY010000016        18584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_2627_length_18584_cov_6.307356, whole genome shotgun sequence.
ACCESSION   DAOMZY010000016 DAOMZY010000000
VERSION     DAOMZY010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 18584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18584
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2627_length_18584_cov_6.307356"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..776)
                     /gene="purE"
                     /locus_tag="PLK64_01930"
     CDS             complement(222..776)
                     /gene="purE"
                     /locus_tag="PLK64_01930"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018774688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPV78528.1"
                     /translation="MSERRSAATSSGDSLGDDPVVGIVMGSDSDWPVMALAAEALDEF
                     GVAYEVDVVSAHRMPEEMIAYGKGAAERGLRVIIAGAGGAAHLPGMLASVTPLPVIGV
                     PVPLAYLDGMDSLLSIVQMPAGVPVATVSVGGARNAGLLAARILGAGEGAEAARMRAE
                     MVRFQGRLRDQAHAKGAALRASRA"
     gene            complement(769..2010)
                     /locus_tag="PLK64_01935"
     CDS             complement(769..2010)
                     /locus_tag="PLK64_01935"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HPV78529.1"
                     /translation="MTSPRRAPGGFPVVGVIGGGQLARMLQAPAVALGVQLSVLAEAV
                     DASAALVIPSAPVGDHADLDAVCRFAASCDVVTFDHEHVPADVLAALEADGVPLHPSP
                     AALRFAQDKLAMRHRLTEIGVACPRWAQARTRADIETFAAVVGWPIVAKTPRGGYDGK
                     GVRVLRGPEDLDVLDAPGEWLEQVGAPGALADGILVEELVPFERELAVLVARSPSGQA
                     SAWPVVQTVQTDGICTEVLAPAPDLDPATAAEATHAALRIAGALGVTGVLAVEMFQVA
                     RAAPGHGDGPAVGERPAYLVNELAMRPHNSGHWSIDGAVTSQFEQHLRAVLDLPLGDP
                     RPVASWTVMANVLGGDYAEIYPAYRHVMARDPGAKVHMYGKGVRPGRKIGHVNVSGPD
                     LADARARAQHAADYLQGVIDE"
     gene            complement(2007..2615)
                     /locus_tag="PLK64_01940"
     CDS             complement(2007..2615)
                     /locus_tag="PLK64_01940"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HPV78530.1"
                     /translation="MSVATGLQRHRRSLRQFVKFGIVGGSGVLVNMLVTIVMHKLHGG
                     TANGRDPLFAIPGTPYFARYLHLVWIGAFLVANVWNFQLNRGWTFRASGHSGWWRQFW
                     PFLAVGSAAAVVGLFVITALTHPQSPIYLPDPYFSESAGLRSRFYWAQLIVIVVTMPI
                     NFVANKLWTFRAVRHHPPGTSAPTVGSSHVADGAPRARQGER"
     gene            complement(2689..3741)
                     /locus_tag="PLK64_01945"
     CDS             complement(2689..3741)
                     /locus_tag="PLK64_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPV78531.1"
                     /translation="MQRIMARAARSALLMGLACAGLPFFGALLWLSTRPDLIDEWLAQ
                     PLGIRALHLAGLLLAMMLIGGTVATVVAGRATESIQAPIDELSRRAEQVGGGETQVPF
                     TPTGVAEIDLVSDALSRSAQRLSRALATERSFASDASHQLRTPLTALMMRIEEIAATD
                     DLAVAHEEAAVAIAQVDRMSRVVDDLLQRRRSDPEGQLPEVSLDTVIATLQREWQPAF
                     ETARRSVRVHGERGLVVRATPIALSQVLSTLLENSLVHGRGTVDVHARRSGPSAVVEV
                     RDEGDGIPAALAPHIFERSVSSGAGSSTGLGLALARDLAESCGGRLDLVARTPATFAL
                     FLSEARDGYQPSASGR"
     gene            complement(3748..4440)
                     /locus_tag="PLK64_01950"
     CDS             complement(3748..4440)
                     /locus_tag="PLK64_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPV78532.1"
                     /translation="MTRVLLVEDDPGISEPLARALRREGYDVDVREDGRAALEGARER
                     PDLVVLDLGLPHLDGLEVCRRMRADGRTMPVLILTARADEVDTVVGLDAGADDYVTKP
                     FRLAELLARVRALLRRTPDGNPVSDTEDLRIDPEGRRAWFQGEELQLTAKEFDLLRVL
                     LREQGRVVTREQLMKEIWDTTWYGSTKTLDMHISVLRRKLGDDVAQPRHISTVRGLGF
                     RFERPESASEPS"
     gene            complement(4519..5301)
                     /locus_tag="PLK64_01955"
     CDS             complement(4519..5301)
                     /locus_tag="PLK64_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPV78533.1"
                     /translation="MLVRIERHGDGGHVAELVLDRPEAMNAVSTAMAEAIAAATSALA
                     ADPSVRCVVLTSSHPKAFCVGADLKERNTMTDADLMAQRPLARAAYGGVLALPMPAVA
                     AVDGFAFGGGLELALSCDLLVAGDGAIVGLPEVSVGVIPGGGGTQLLARRIGWSRAAR
                     LIFTAVKLPAAEAATFGVIDELVPAGTARERALAIATQIAANSPVGLRSAKRAMRLGL
                     DVDLASGLEIEDACWRATAFSGDRAEGVAAFAEKRAPRWPGR"
     gene            complement(5364..6458)
                     /locus_tag="PLK64_01960"
     CDS             complement(5364..6458)
                     /locus_tag="PLK64_01960"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPV78534.1"
                     /translation="MTVGPAAHNEGMLPSTAAAARTADSLNSDDVASLLVGTSREFSR
                     SDVSRLAGVSVVGARKFWHALGFASTTSSDRMFTEADLRALRRMNMLVRSSVIDEATA
                     LALTRAFARNTDRLAAWQVQLISEAMTASSGVADVDGPPNEAIAQRTARLIVDLADEL
                     EPMLVYAWRRHLADAVSRMLADVGSGSESDAPGQLRCVGFADLVSFTTLVRQLSERDL
                     ARTVQRFEALATDVVTAHGGRVVKTVGDEVLFSTRTPAPGAAIALDLVDALAEDEVLP
                     DLRVGVSFGTVVSRLGDVFGTTVNRAARLTALAHPNAVLVDGPLATALTSQSGFRLTA
                     MRRRTLRGVGSVTPHVLARTSTPTRRGRYE"
     gene            complement(6466..7299)
                     /locus_tag="PLK64_01965"
     CDS             complement(6466..7299)
                     /locus_tag="PLK64_01965"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPV78535.1"
                     /translation="MAHPMGNRSDLDVARLTSRLCRPPRTWASVDVFDELMSTNALAM
                     VRGQVWAVVVAEHQFSGRGRLDRQWVAPTGTSLTFSATVPAPTEAPGWVPLVAGLAVA
                     EAVVAVTGLPAVLKWPNDVLLPDDGHRKVCGVLCEYRPPADGADALVVVGIGLNVSQT
                     RDQLPVDTGTSLTLAGAEPVDREDLLVTVLDRIAVRYAALQAGGAAADGVRAAYRAAC
                     STLGTEVRLERPGAGTSTAHALDIDADGALVVDEGSGPTRYAAGDVTHVRPASGGGGL
                     A"
     gene            7323..8942
                     /locus_tag="PLK64_01970"
     CDS             7323..8942
                     /locus_tag="PLK64_01970"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012802303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPV78536.1"
                     /translation="MSNAGVPDAAAPTLDLSTTAGKLAELRRRVAEVANAGSTTAVEK
                     QHAKGKKTARERIAMLLDEGTFIEMDKYARHRSNQFGQEKHRPYGDGVVTGYGQVDGR
                     QIAVFAQDFTVFGGSLGEVFGEKIVKVMDWAAKIGVPVVGLNDSGGARIQEGVVSLGL
                     YGEIFFRNVRSSGVVPQISVIMGPCAGGAVYSPAITDFTVMVDKTSHMFITGPDVIKT
                     VTGEEVEFEDLGGARAHNTKSGVAHYMGADEDDAIDYVKALLSYLPSNNMELPPAFGD
                     GESDLAPTDDDLWLDTCIPDSPNQPYDMHEVIRHVVDEGEFLEVHALFAPNIIVGFAR
                     VDGMSIGIVANQPMQFAGCLDIEASEKAARFVRTCDAFNVPVVTFVDVPGFLPGTDQE
                     WNGIIRRGAKLIYAYAEATVPKITVITRKAYGGAYDVMGSKHLGADINLAWPTAQIAV
                     MGAQGAVNILYRKELAKAREEGRDVEQARADFIKLYEQTLANPYIAAERGYIDTVISP
                     SNTRMNVTRALRALRTKRESLPPKKHGNIPL"
     gene            8939..9184
                     /locus_tag="PLK64_01975"
     CDS             8939..9184
                     /locus_tag="PLK64_01975"
                     /inference="COORDINATES: protein motif:HMM:NF025192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit epsilon"
                     /protein_id="HPV78537.1"
                     /translation="MTVRTTMTSDAATDPTPPVVRVLHGDPTPEELGIVVALLSAMGG
                     DDAAESPRSESRWSGPAARLGSAGPGRDSWRFSGMPR"
     gene            complement(9288..>9560)
                     /locus_tag="PLK64_01980"
     CDS             complement(9288..>9560)
                     /locus_tag="PLK64_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ligand-binding protein SH3"
                     /protein_id="HPV78538.1"
                     /translation="STFGITNIGGYRAGDPRDHGTGHAVDVMITNRATGDAVAAYVIA
                     HASEFHVKYIIWRQRIWFPGGSWKAMADRGGVTANHYDHVHISVTS"
     assembly_gap    9561..9660
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9661..9992)
                     /locus_tag="PLK64_01985"
     CDS             complement(<9661..9992)
                     /locus_tag="PLK64_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78539.1"
                     /translation="MSGHERYNGRHRAPGRHRAPRAAVRVPHRAGAAAIGVGLIASAV
                     TVPAVGMANAFGADSAEAEAVAFAQLQLISASPTSVTRADSGVVVSRSRSRESIEDID
                     EPEAAVVAP"
     gene            complement(10068..10922)
                     /gene="rfbD"
                     /locus_tag="PLK64_01990"
     CDS             complement(10068..10922)
                     /gene="rfbD"
                     /locus_tag="PLK64_01990"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HPV78540.1"
                     /translation="MKILITGANGMLGTDLTRVLREAGHEVTPTDLPEVDITDPASCA
                     AAVAGHDVVVNTAAYTAVDRAESDEATAFRINAVGPATLARAVHAAGARMVHISTDYV
                     FAGDGSTPYAEDAPIAPKSAYGRTKAAGEWAVRAECPQSYIVRTAWLYGEHGPNFVKT
                     MARLAGERDTVSVVSDQIGQPTWTVDLSRYIAEVLGAEVPFGTYHGTSEGETTWFGFA
                     QAVFEELGLAPDRVRPISTDEFPQPAPRPAYSVLGHNRTDAVGVARLPHWRVRLAETV
                     ADVVRDTR"
     gene            10972..11847
                     /gene="rfbA"
                     /locus_tag="PLK64_01995"
     CDS             10972..11847
                     /gene="rfbA"
                     /locus_tag="PLK64_01995"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HPV78541.1"
                     /translation="MKGIILAGGSGTRLHPITKGISKQLMPVYDKPMIYYPLSTLMLA
                     GIREVLVITTPEDSTQFQRLLGDGREWGIELTYAVQPSPDGLAQAFIIGADFIGTDSV
                     ALVLGDNIFYGASLEEQLRGGADITGGHVFAYHVANPQDYGVVEFDAQGHVLSIEEKP
                     ARPKSSYAVPGVYFYDNSVVGVARELQPSERGELEITGVNDVYLKRGNLTVTVLPRGT
                     AWFDTGTFQGLLDASQFVHVVEARQGHKIGCVEEIAWRNGWIDDAQLTVLADRLAKSG
                     YGVYLKGLLVEKGDS"
     gene            11847..12443
                     /gene="rfbC"
                     /locus_tag="PLK64_02000"
     CDS             11847..12443
                     /gene="rfbC"
                     /locus_tag="PLK64_02000"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HPV78542.1"
                     /translation="MQFRPLRVEGAIEVTPRQFPDSRGMFAEGFRADKLAEHIGFELA
                     VRQTNISVSSAGAVRGIHYASLPPSQAKYVTALSGVFLDFIVDIRVGSPTFGQWDVVR
                     LDTVDRRAVYLSEGLGHVLACLEDGTAMYLCSEVYNPAGEKGVTPLDPTINLGLPADF
                     APIVSEKDAAAPTLDEALAAGLLPDYAECLAFRQSLHA"
     gene            12449..12970
                     /locus_tag="PLK64_02005"
     CDS             12449..12970
                     /locus_tag="PLK64_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HPV78543.1"
                     /translation="MPPASSAPTPPPEPTPAHHRITVQATRLARTTATTVASHARQVP
                     ARVQRLRTTTVPRLGLATVHGRSMEPTLHEGDRLLVLWGGPIVPGRMSLVRLPDSPGG
                     PRPLSVKRVSGPEADQPGRWWIERDNPREGVDSWQVGGIPSEDVVALVVTRLPAFPAL
                     RGSLRRLTRGRLG"
     gene            13023..13424
                     /gene="sodN"
                     /locus_tag="PLK64_02010"
     CDS             13023..13424
                     /gene="sodN"
                     /locus_tag="PLK64_02010"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HPV78544.1"
                     /translation="MRLRFFATAVEVSAHCDLPCGVYDPAQARIEAQSVKATIEKALA
                     SDDPDFKVRAMIIKEQRSHLVKEHLWVLWTDYFKAPHFEKYPQLHTLINDATKLAGAG
                     GTKGSWDLAKADELLAKIDEIAEIFWETKKA"
     gene            complement(13484..14824)
                     /locus_tag="PLK64_02015"
     CDS             complement(13484..14824)
                     /locus_tag="PLK64_02015"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPV78545.1"
                     /translation="MNAPDIALGVVRGPAHGVRRRLTARWVVHVAVAVAVIGTINTIR
                     NPGPFLSAWLVVSSFAVLIGVGEYFRFPVEDGRQLAPMTLATGLAFALTSVSWQVDLT
                     HLAPGPIVMVSAIAMLCGALPHAARGRSLRVEEMAARFLAIFVTALLFRWAPLWSGRT
                     LLEVDATWVGSRWPVAVLLAVIAAIGLFTFLGLSALVMASRSAGSLRQAFLDEVRSGA
                     GLGLALSATGALIALAERPLGVLALPLFLVPLVLTQFALRQYAAIRATYAQTVRVLSR
                     LTEMGGFTRAGHPDRVAELCVAVGRDLGLSGRDIRNLENAALLHDIGQVALRAPIPAG
                     ATLMAAPADQRRIASDSADIVRKTGLPPEVAIAVEHQATPYRVVREQRQQVPMLSRIV
                     KVANAYDDLVGGSIALRRREAAVERIHLGLGYEYDPRVVDSLVRVLARRHTKGP"
     gene            complement(14821..16221)
                     /locus_tag="PLK64_02020"
     CDS             complement(14821..16221)
                     /locus_tag="PLK64_02020"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPV78546.1"
                     /translation="MSPQAAADPGPRARRVNAVILATLVVAVLITTLAWALDGVPHDY
                     VSLAVLTVLTLGTRVVRVRVVERVAVAASGIVSLASCVLVGPVGSALMMATTILLERG
                     AAGWRARLFNASMGAILGSVGGLTYLLAGGPADLTTISGPGELMVRVGGPFLVASLVF
                     SLVNFASIAMIVRVDSGESFQSMFLAMLTTSGLAQMGYGVIGLLFVILWVPAQVGPFS
                     AVLILLPLFVAQWAFVQYAEEERAHERTLAALVAAGETRDPYAVGHSERVARLSVLLG
                     EGLGLGPAQTEALQYAAILHDIGWLGAQAWAGSDTKAPDKSLSELIARHPAVGVALLS
                     DIAFLQDSLDSIKHHHERWDGRGYPDQLVGTDIPLASRIIAVADSFDSLTRGRPGRQA
                     LPSNRALQVVESRAGTHLDPTVVAVLARVLERHTWAVAEPPPEGAGAPLWDHDDPVMS
                     DMLAGIDRPATAGQGS"
     gene            complement(16316..16492)
                     /locus_tag="PLK64_02025"
     CDS             complement(16316..16492)
                     /locus_tag="PLK64_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78547.1"
                     /translation="MKTVSKLFEAMLTKETTPVWGGRAATRVDGPTGMFTWEPKGKID
                     GPLAMFTWEPPKRG"
     gene            complement(16562..16738)
                     /locus_tag="PLK64_02030"
     CDS             complement(16562..16738)
                     /locus_tag="PLK64_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78548.1"
                     /translation="MKTVSKLFEAMLTKETTPVWGGRAATRVDGPTGMFTWEPKGKID
                     GPLAMFTWEPPKRG"
     gene            complement(17229..17471)
                     /gene="rsrA"
                     /locus_tag="PLK64_02035"
     CDS             complement(17229..17471)
                     /gene="rsrA"
                     /locus_tag="PLK64_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HPV78549.1"
                     /translation="MDCSEALLRAFEFLDGEMEANDFAKVRAHLDACAECLRQYDLDH
                     MVKLMIKRSCAPEPAPTALRATIVSRLTVIRIETTN"
     gene            complement(17630..18349)
                     /locus_tag="PLK64_02040"
     CDS             complement(17630..18349)
                     /locus_tag="PLK64_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPV78550.1"
                     /translation="MTTDPASPTPSDATGSLAASSTEVLPETDLPVVADAAPALDLAT
                     ETTEERRARFEREAMPLLDQLYSAALRTARNAADAEDLVQETYAKAWAAFHQYQPGTN
                     LKAWMYRILTNTYINSYRKKQREPLQSDAAEIEDYQLHRADSHSSAGGRSAEDVALDH
                     LPDSDVKRALLAIPEEFRLAVYFADVEGYAYKEIAEIMQTPIGTVMSRLHRGRRQLRD
                     LLTEYAAERGYVRTDVQTEGR"
BASE COUNT         3076 a         6457 c         6294 g         2657 t
ORIGIN      
        1 gcccccccgc gccccacgac tcccacgacc cccgccacgc gccccacgtg ccccacgtgc
       61 cccccgcgcc ccacgcgccc cacgccaccc accccggaga tagcgctcgc acggtcatct
      121 ttccgtcaac tcaggcccga aatgacccca cagatgaccg tgcgagcgct atctccgggt
      181 caggatgggg gtgggagacg cgcgaataca gatgtatgcc gtcaggctcg ggaggctcgt
      241 agcgcggcgc ccttggcgtg ggcctggtcg cgcaaccgcc cctggaagcg caccatctcg
      301 gcgcgcatcc gagccgcctc ggcaccctca ccggcgccga ggatccgcgc cgccaacagg
      361 ccggcgttgc gtgcgccgcc caccgacacg gtcgcgaccg gcaccccggc aggcatctgg
      421 acgatcgaca acagggagtc catcccgtcg agataggcca acggcaccgg cacgccgatg
      481 acgggcagcg gcgtgaccga cgcgagcatc cccggcaggt gggccgctcc cccggccccg
      541 gcgatgatga cccgcaggcc ccgctcggcc gcccccttgc cgtaggcgat catctcctcg
      601 ggcatccggt gggcagacac gacatcgacc tcataggcga ccccgaactc gtcgagagcc
      661 tcggccgcca aggccatcac cggccagtcg ctgtcgctgc ccatgacgat gccaacgacc
      721 gggtcgtccc cgagcgagtc accggaagac gttgcagcag aacgacgttc actcatcgat
      781 gactccttgc agatagtcgg cggcatgctg agcacgagcg cgggcatcgg ccaggtcggg
      841 cccactgacg ttgacatggc caatcttgcg accgggccgg acccccttgc catacatgtg
      901 caccttcgcc cccgggtcac gcgccatgac gtgccggtaa gcggggtaga tctcggcata
      961 gtcgccgccg aggacgttcg ccatgaccgt ccacgaggcc accggccgcg ggtcaccgag
     1021 cggcagatcg agcacggccc gcaggtgttg ctcgaactgg ctggtcacgg cgccgtcgat
     1081 gctccaatgc ccactgttgt gggggcgcat cgcgagctcg ttgacgaggt atgccggtcg
     1141 ctcgccgacc gcggggccgt ccccgtggcc gggcgcggcg cgggcgacct ggaacatctc
     1201 gacggcgagc acccccgtca cgccgagtgc gccggcgatg cgcagcgcgg cgtgggtggc
     1261 ctcggcggca gtcgccggat ccagatccgg cgcgggagcg aggacctcgg tgcagatgcc
     1321 gtcggtctgg acggtctgca cgaccggcca ggcggatgcc tgcccactgg ggctgcgggc
     1381 aacgagcacc gccagctcgc gttcgaacgg cacgagctct tccaccagga tcccgtcggc
     1441 gagcgcccct ggcgcaccga cctgctccaa ccactcgcct ggcgcatcga ggacgtcgag
     1501 gtcctccggg ccgcgcagca cccggacgcc cttgccgtcg tatccacccc ggggggtctt
     1561 ggcgacgatc ggccagccga caaccgcagc gaacgtctcg atgtccgccc gggtgcgggc
     1621 ttgcgcccag cgcgggcagg ccacgccaat ctccgtcagc cgatgacgca tggccaactt
     1681 gtcctgagcg aagcgcagcg ctgccgggct gggatgcaac gggaccccgt cggcttccag
     1741 agcggccagc acgtcggccg ggacgtgctc gtggtcgaag gtgacgacgt cacaggaggc
     1801 ggcgaaccgg catacggcat cgaggtcggc gtggtcaccc actggagccg acgggatgac
     1861 cagggccgca gacgcgtcga ccgcttcggc gagaacgctc agctgcacgc cgagcgcgac
     1921 cgccggggcc tggagcatcc gcgctagttg cccaccaccg atgaccccca cgacggggaa
     1981 gccgccggga gcgcgccgcg gcgacgtcac cgctccccct gccgggcgcg aggtgcgccg
     2041 tcggcgacgt ggctcgaacc gaccgtggga gccgaagtac ccggcgggtg gtgccggacg
     2101 gcacggaagg tccacaactt gttggcgacg aagttgatgg gcatggtcac gacgatgaca
     2161 atcaactggg cccagtaaaa ccgcgagcgc agaccggccg actcgctgaa gtacgggtcg
     2221 ggcaggtaga tcggtgactg cgggtgggtg agcgccgtga tgacaaacag gccgacaacc
     2281 gcggcggcgc tgcctacggc gaggaacggc cagaactgac gccaccagcc agagtgcccg
     2341 gaggcccgga acgtccagcc acggttgagc tggaagttcc agacgttggc gacgaggaag
     2401 gccccgatcc acaccagatg caggtagcgg gcgaagtagg gcgtcccagg gatagcgaac
     2461 aacggatccc gcccgttggc cgtgcccccg tggagtttgt gcatgacgat ggtcaccaac
     2521 atgttgacca ggacgcccga gccgccgacg atgccgaact tgacgaactg gcgaaggctg
     2581 cgccggtgtc gctgcagtcc cgtcgctacg ctcacggtct cggagcctac cctccggccc
     2641 ggtgaccgcc tcccgagcga ccgctccctg ggcggcccac caactccgtc accgaccgct
     2701 cgccgacggt tgatacccgt cgcgggcctc ggagaggaac aacgcgaagg tggcgggcgt
     2761 ccgggcgacg aggtctagcc gcccgccaca cgactcggcc aggtcgcgcg ccaacgccaa
     2821 cccgagcccg gtgcttgatc cagctccgct gctcaccgac cgctcgaaga tgtgcggcgc
     2881 aagagcagcg ggtatgccgt ctccctcgtc gcgcacctcg accaccgcgg acggaccgct
     2941 gcgtcgagca tgcacgtcga cggtgccccg tccgtggacc aaggagttct ccagcagcgt
     3001 cgagaggact tggctcaagg cgatcggggt cgctcgcacg accaggccgc gctcgccgtg
     3061 gacgcggaca ctgcgtcgag cggtctcgaa cgcgggctgc cactcgcgtt gcagggtcgc
     3121 gatgacggtg tcgagcgaca cctccggcaa ctgcccttcg gggtcgctgc gccgccgctg
     3181 caacaggtcg tcgacgaccc ggctcatccg gtcgacctgg gcgatggcga cggcggcctc
     3241 ctcgtgggcc accgcgaggt cgtcggtcgc ggcgatctcc tcgatccgca tcatcagtgc
     3301 ggtgagcggg gtgcgcagct ggtgcgaggc gtcggaggcg aagctgcgct cggtggccaa
     3361 cgcccgagag agccgctgcg ctgatcggga cagggcatcg gagacgaggt caatctcggc
     3421 gacccccgtc ggcgtgaacg gcacctgggt ctccccgccg ccgacctgct cggccctgcg
     3481 gctcagctcg tcgatcgggg cctggatcga ctcggtcgcg cggccggcga ccaccgtcgc
     3541 gaccgtccct ccgatgagca tcatcgcgag caggagcccg gctaggtgca gggcgcggat
     3601 gccgagcggt tgggccaacc actcgtcaat caggtcgggg cgggttgaca gccacagcag
     3661 ggctccgaaa aacggcaggc ctgcgcaggc caaacccatg agcagggcgg atcgggccgc
     3721 gcgcgccatg atccgctgca tgacaggtca gctcggctcg ctggcgctct cgggacgttc
     3781 gaagcggaac ccgagtccgc gcaccgtcga gatgtgccga ggttgggcca cgtcgtcgcc
     3841 gagtttccgt cgcaatactg agatgtgcat gtcgagggtc ttggtcgacc cgtaccacgt
     3901 ggtgtcccag atctccttca tgagctgttc gcgggtgacc acccggccct gctcacgaag
     3961 cagcacccgc aacaggtcga attcctttgc ggtcagctga agttcctcac cctggaacca
     4021 cgcgcgccga ccctcggggt cgatacgaag gtcctccgtg tcggagacgg ggttgccgtc
     4081 aggggtccgc cgcagcaggg cgcggacgcg agcgagcaac tcggccaggc ggaacggctt
     4141 ggtgacgtag tcgtcggcac cggcatcgag cccgacgacg gtgtcgacct catcggcccg
     4201 cgccgtgagg ataagcaccg gcatggtgcg gccgtcagcc cgcatgcggc ggcatacctc
     4261 caggccgtcc aggtgcggga ggccgaggtc caggacaacg agatcgggtc gttcccgcgc
     4321 gccttcgagg gcagcccggc cgtcttcacg cacatcgacg tcgtacccct cacgtcgcag
     4381 cgcgcgcgcc aggggctcag agatgcctgg gtcgtcctcg acgagcagca cccgggtcat
     4441 gggccagatg gtatgccttc ggacctcacg ccgcatgtac ggccggtgca gcccgacctc
     4501 ctatgagcgc aattcgggtc agcgaccggg ccaccgggga gcccgcttct cggcgaaggc
     4561 ggcaacccct tcggcacggt caccgctgaa ggccgtagcc cgccagcagg cgtcctcgat
     4621 ctccaggccg gaagccaggt cgacatcaag gcccaggcgc atggctcgct tggccgagcg
     4681 caaccccacc ggtgagttgg ccgcgatctg ggtggcgatt gccagggccc gctcccgagc
     4741 cgtcccggcc ggcacgagct cgtcgatgac cccgaacgtc gcggcctcgg cagccgggag
     4801 tttgaccgcg gtgaagatca accgagccgc tcgggaccag ccgatccgac gcgcaagcag
     4861 ttgggtgccg ccgcctccgg ggatcactcc gacagacacc tcgggcaggc cgacgatcgc
     4921 gccgtcgccg gcgacgagga ggtcgcagga gagggcgagc tccagcccgc cgccgaacgc
     4981 gaacccgtcg acggcagcca ccgcaggcat cggcagggca agaaccccac cgtatgccgc
     5041 ccgcgccagg ggacgctggg ccatgaggtc ggcgtcggtc atcgtgttgc gttccttgag
     5101 gtcggccccc acgcagaacg ccttcgggtg cgaggaggtc aggacgacgc agcgcacgct
     5161 cgggtcggcc gcgagggcac tcgtcgctgc ggcgatcgcc tcggccatgg ccgtcgagac
     5221 cgcgttcatc gcttcaggcc gatccaggac gagctcggcg acgtggccgc cgtcgccgtg
     5281 ccgttcgatg cgcaccaaca ggcgcggttc ttgcggacag acggtcgtgc cgatgggctg
     5341 tgggtccggg ccggcgctgc tggtcactcg tatctccctc gtcgggtcgg ggtgctcgtc
     5401 cgagcgagca cgtggggggt cacggagccc acgccgcgca gcgtgcgacg ccgcatcgcg
     5461 gtgagccgga agccggactg actggtgaga gccgtcgcca gcggtccgtc gacgaggacg
     5521 gcgttgggat gggcaagggc cgtcagccgg gcggcccggt tgacggtcgt cccgaagacg
     5581 tcgcccaacc gggacacgac agtgccgaac gagaccccga cccgcaggtc cggcagcacc
     5641 tcatcctcgg cgagcgcgtc gaccaggtcg agggcgatgg ccgccccggg cgcaggggtg
     5701 cgcgtcgaga agagcacctc gtcgccgacc gtcttgacca cccgtccgcc gtgcgcggtg
     5761 accacgtccg tggccagggc ctcgaaccgc tggacggtcc gagcgaggtc gcgttcggac
     5821 agttggcgca ccagcgtcgt gaacgacacg agatcggcga aacccacaca tctcagttgg
     5881 cctggggcgt cgctctcgga gcccgagccc acgtcggcga gcatgcgcga cacagcatcg
     5941 gcgaggtgcc gacgccaggc atagacgagc atcggctcga gttcgtcggc caggtcgacg
     6001 atcagtcggg cggtgcgctg cgcaatcgcc tcgttgggcg gcccgtccac gtcggctacc
     6061 cccgaggacg ccgtcatcgc ctcggagatg agttgcacct gccaggccgc cagccgatcg
     6121 gtgttccggg cgaaggctcg ggtgagcgcg agcgcggtcg cctcgtcgat gacgctgctg
     6181 cgcaccagca tgttcatccg gcgcagggcc cgcaggtcgg cctcggtgaa catccgatcg
     6241 ctggaggtcg tcgaggcgaa gccgagggcg tgccagaact tccgcgcgcc gacgaccgac
     6301 acccccgcca gtcggctgac atcgctgcgg gaaaactcgc ggctcgtgcc caccagcaac
     6361 gacgccacgt cgtcgctgtt caaggagtcg gcggtccggg cggctgcggc cgtcgacggc
     6421 agcatgccct cgttgtgcgc cgccggaccg accgtcatag aagcatcatg ccaacccgcc
     6481 gcccccggaa gcgggccgca cgtgcgtcac atcgcccgcg gcatacctcg tcggtccgga
     6541 accctcgtcg acgacgagcg cgccgtcggc atcgatgtcc agcgcgtgcg ccgtcgaggt
     6601 gcccgccccg gggcgctcca accgcacctc ggtgcccagg gtcgaacagg ccgcgcggta
     6661 tgccgcccgc accccgtccg ccgccgcccc acccgcctgc agcgcggcat accggacggc
     6721 gatccggtcc agaacggtga cgagcaggtc ctcgcggtcg accggctcgg cgcccgcgag
     6781 ggtgagcgac gttcccgtgt cgacggggag ttggtcccgg gtctggctga cgttgagtcc
     6841 gatgcccacg acgacgagtg cgtcggcacc gtcggccggg gggcgatatt cgcacaagac
     6901 gccgcagacc ttgcggtgcc cgtcatcggg cagcagcacg tcgttcggcc acttgagcac
     6961 ggcgggaagg ccggtcaccg ccacgacggc ctcggcgacc gccaggcccg cgacgagcgg
     7021 cacccacccg ggcgcttccg tcggtgccgg caccgtcgcc gagaaggtga gcgaggtgcc
     7081 cgtcggcgcc acccactggc ggtccagccg cccccgaccg gagaactgat gctcggcgac
     7141 gacgaccgcc cagacctgcc cgcggaccat cgcgagcgcg ttggtcgaca tcaactcgtc
     7201 gaagacgtcg accgacgccc aggttcgcgg gggtcgacac agacgggagg tgagacgagc
     7261 gacatcgagg tcactccggt tccccatggg atgcgccatg agtcaaggct attgttcgga
     7321 tcatgtcgaa cgctggtgtt cctgacgcgg ccgctcccac cctcgacctg tcgaccaccg
     7381 ccggcaagct tgccgagctg cggcgccggg tcgccgaagt cgccaatgcc ggctcgacga
     7441 ctgcggtgga gaagcagcac gccaagggca agaagaccgc ccgcgagcgg atcgcgatgc
     7501 tcctggacga gggcacgttc attgagatgg acaagtacgc ccgccaccgg agcaaccagt
     7561 tcgggcagga gaagcaccgg ccctatgggg atggcgtcgt caccggctac ggccaggtcg
     7621 acggtcgcca gatcgcggtg ttcgcccagg acttcaccgt tttcggcggc tcgctcggcg
     7681 aagtcttcgg cgagaagatc gtcaaggtga tggactgggc cgccaagatc ggcgtccccg
     7741 tcgtcggcct caacgactcc ggcggcgccc gcatccagga gggtgtcgtc tcgctcgggc
     7801 tctacggcga gatcttcttc cgcaacgtac gctcctcggg agtcgtcccg cagatctcgg
     7861 tcatcatggg cccgtgtgcg ggcggggccg tctactcccc cgcgatcacc gacttcacgg
     7921 tcatggtcga caagacctca cacatgttca tcaccggccc cgacgtcatc aagaccgtca
     7981 ccggtgagga ggtcgagttc gaggacctcg gcggcgcgcg cgcccacaac accaagtccg
     8041 gtgtcgccca ctacatgggt gccgacgagg acgacgccat cgactacgtc aaggcgctgc
     8101 tgtcctacct gccgagcaac aacatggagt tgcccccggc cttcggcgac ggcgagtcgg
     8161 acctcgctcc gaccgacgac gacctgtggc tggacacctg catccccgac tcgcccaacc
     8221 agccttacga catgcacgag gtcatccggc acgtcgtgga cgagggcgag ttccttgagg
     8281 tccacgcgtt gttcgccccg aacatcatcg tcgggttcgc ccgagtcgac gggatgagca
     8341 tcgggatcgt cgccaaccag cccatgcagt tcgccggatg cctggacatt gaggcctccg
     8401 agaaggccgc ccggttcgtg cgcacgtgcg acgccttcaa cgtccccgtg gtcaccttcg
     8461 tcgatgtgcc gggcttcctg cccggcaccg accaggagtg gaacggcatc atccgtcgcg
     8521 gtgccaagct catctatgcc tacgccgagg cgaccgttcc caagatcacc gtcatcacgc
     8581 gcaaggccta tggcggcgcc tacgacgtca tggggtccaa gcacctgggc gccgacatca
     8641 acctcgcctg gccgaccgct cagatcgccg tcatgggtgc gcagggcgca gtcaacatcc
     8701 tctaccgcaa ggagttggcg aaggctcgcg aggaaggccg cgacgtcgag caggcccggg
     8761 ccgacttcat caagctgtat gagcagacgc tcgccaaccc ctacatcgcg gccgagcggg
     8821 gttacatcga cacggtcatc agcccgtcca acacccggat gaacgtcacc cgggcgctgc
     8881 gggcgctgcg caccaagcgc gagagcctgc ccccgaagaa gcacggcaac atccccctgt
     8941 gaccgtgagg accacgatga ccagcgacgc cgccaccgac ccgacaccgc ccgttgttcg
     9001 cgtgctgcac ggtgacccca cgccggagga gctcggcatc gtcgtcgcgc tgctgtcggc
     9061 gatgggtggc gacgacgcgg ccgagtcccc gcgtagcgag tcacggtggt cgggtccggc
     9121 cgctcgactc ggcagcgccg gtcccggtcg cgacagttgg cgattctccg gtatgccgcg
     9181 ctgaccccct cgtgagctgc ctgtggttgg gcgagccgtt gctgcacccg gaaccgcgtg
     9241 gatgagtcag ctcgcggaag cgtcagccaa ctcgggggaa gatccggtca gctcgtgacc
     9301 gagatgtgca cgtggtcgta gtggttcgcc gtgaccccgc cgcggtcggc catggccttc
     9361 cacgagccgc ccgggaacca gatccgctgg cgccagatga tgtacttcac gtggaattcg
     9421 ctcgcgtgcg caatgacgta ggccgcgacg gcgtcgcccg tcgcacggtt ggtgatcatc
     9481 acgtcgaccg catgaccagt gccgtggtcg cgcgggtcgc ctgcgcggta cccgccgatg
     9541 ttggtgatcc cgaacgtgga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 ggggccacga ccgccgcctc cggctcatcg atgtcctcga tcgactcgcg cgatctggac
     9721 cgggacacga ccacaccgga gtccgcccgg gtgacggagg tcggcgaggc cgagatcaat
     9781 tgcaactgcg cgaaagccac tgcttcggcc tcggcggagt cagctccgaa agcgttggcc
     9841 atgcccaccg cgggcacggt cacagcgctg gcgatcaggc cgacaccgat cgcggcggcc
     9901 ccggcgcggt gtggcaccct gaccgcggcg cgcggggcac ggtgtcgccc gggagctcgg
     9961 tggcgcccgt tgtaacgctc gtgtccggac atggtgtcct cgggtggaag tcgggtgatg
    10021 ttcgtccaga ctttactgca caaagccggg gaggacacgc tcccccacta acgggtgtcc
    10081 ctcacgacgt ccgccacagt ctcggccagc cggactcgcc aatggggcag gcgcgcgaca
    10141 cccacggcat cggttcggtt gtgcccaagc acgctgtagg ccgggcgagg cgccggctgc
    10201 ggaaactcgt ctgtcgagat tggccgcacc cggtcgggtg ctagcccgag ctcctcgaag
    10261 actgcttggg caaacccgaa ccacgtcgtc tcaccctcag acgtcccgtg gtatgtgccg
    10321 aaagggacct ctgcgccaag aacctccgcg atgtaccgcg acagatcgac cgtccaggtg
    10381 ggctgaccga tctgatcgga aacaaccgag acggtgtcgc gctcgccggc caatcgcgcc
    10441 atcgtcttga cgaagttcgg accgtgctcg ccgtagagcc aagcggttcg cacgatgtag
    10501 gactgcgggc actcggcccg cacggcccac tccccggcgg ctttggtgcg tccataggcg
    10561 gacttcggcg cgatcggtgc gtcctcggca tacggggtgc tcccgtcgcc ggcgaagacg
    10621 tagtcggtgg agatgtgcac catccgcgcg ccggcggcgt gcaccgcgcg ggcgagggtg
    10681 gccgggccga ccgcgttgat ccggaaggcg gtcgcctcgt cgctctcagc ccggtcgacc
    10741 gcggtgtatg ccgcggtgtt gacgacgacg tcgtggcccg ccactgcggc cgcgcacgag
    10801 gcggggtcgg tgatgtccac ctcggggagg tcggtcgggg tcacctcatg accagcctcg
    10861 cgcagcaccc gagtgaggtc tgtgccgagc atgccgttgg ctccggtgat gaggatcttc
    10921 acatcgtgga gtctagtgag agcctcctcg cgcgcccgct agcctggagc gatgaagggc
    10981 atcatcctgg ccggcgggtc gggcacccga ctacacccga tcaccaaggg catcagcaag
    11041 caactcatgc cggtctacga caagccgatg atctactacc cgctgtcgac gctcatgctt
    11101 gcgggtatcc gcgaagtcct cgtcatcacg accccggagg actcgacgca gttccagcgt
    11161 ctgctcggcg acgggcgcga atggggcatc gagctcacgt atgccgtgca gcccagcccc
    11221 gacggcctgg cccaggcgtt catcatcggc gccgatttca tcggcaccga ctcggtcgcg
    11281 ctggtccttg gcgacaacat cttttatggc gcctcacttg aggaacagtt gcgtgggggt
    11341 gccgacatca ccggcgggca cgtgttcgcc taccatgtgg ccaaccccca ggactatggc
    11401 gtcgtcgagt tcgacgcgca ggggcatgtc ctgtcgatcg aggagaagcc agctcggccc
    11461 aagtccagtt acgccgtgcc gggggtctac ttctacgaca actcggttgt cggagtcgcc
    11521 cgggagctcc agcccagtga gcgcggcgag ttggagatca ccggcgtcaa cgacgtctac
    11581 ctcaagcgtg gcaacctcac ggtcaccgtg ctcccccggg gcaccgcgtg gttcgacacg
    11641 gggaccttcc aggggttgct cgacgcgagc cagttcgtcc acgtcgtcga ggcccgccag
    11701 ggtcacaaga tcggctgcgt cgaggagatc gcgtggcgca acggctggat cgacgacgcc
    11761 caactcacgg tcctcgccga ccggctcgcc aagagcggct acggcgtcta tctcaagggt
    11821 ttgctcgtcg agaagggtga ctcgtaatgc agttccgccc gttgcgcgtc gaaggcgcca
    11881 tcgaagtgac cccacgccaa ttccctgaca gcagagggat gttcgccgag ggtttccggg
    11941 ccgacaagct tgccgagcac atcggcttcg agttggccgt gcgccagacc aacatcagcg
    12001 tgtccagcgc cggcgccgtc cgaggcatcc actacgcctc gttgccgccg tcgcaggcga
    12061 agtacgtcac cgcgctctcg ggcgtcttcc tcgacttcat cgtcgacatc cgggtcggct
    12121 cgccgacctt cggccaatgg gacgtcgtgc ggctcgacac tgtcgaccgc cgcgcggtct
    12181 atctctccga ggggctcggc cacgtgttgg cctgcctcga ggacggcacc gcgatgtacc
    12241 tctgcagcga ggtctacaac cccgccggcg agaagggcgt cacccccctc gacccaacga
    12301 tcaacctcgg cctgcccgcc gacttcgccc cgatcgtgtc cgagaaggac gccgccgcac
    12361 cgaccctcga cgaggcgctg gcggcgggcc tgctccccga ctacgccgaa tgcctcgcct
    12421 tccggcaatc cctccacgcg tgagcggtat gccgccggcc tcctccgcgc cgactccgcc
    12481 gcccgagccg accccggcgc accatcgcat caccgtccag gccacccggt tggctcggac
    12541 gaccgcgacc accgtcgcgt cccacgcccg gcaggtgccc gctcgcgtcc aacggctgcg
    12601 cacgaccacg gtgccccgcc tgggcctagc caccgttcac ggccgctcga tggagccgac
    12661 cctgcacgag ggcgaccgac tcctcgtcct ctggggtggg ccgatcgtgc cgggacggat
    12721 gtccctcgtg cgactgccgg acagcccagg cggcccccga cccctgtcgg tcaagcgggt
    12781 ctccgggccc gaggccgacc agccgggtcg ctggtggatc gagcgggata acccccgcga
    12841 gggcgtcgat tcctggcagg tgggcggcat accgagtgag gatgtcgtcg cgctggttgt
    12901 gacgcgccta cccgcattcc ctgccctacg aggttcgctg cggcggctca cgcggggtag
    12961 gttggggtaa tccggcgagc cgcccgaggc acccccggat cgacgacgaa gggacaccca
    13021 ccatgcgttt gcgcttcttc gcgacggccg tcgaggtcag cgcccactgc gacctgccgt
    13081 gcggcgtcta cgaccccgct caggcccgca tcgaggccca gtcggtcaag gccaccatcg
    13141 agaaggccct ggccagcgac gaccccgact tcaaggtccg cgccatgatc atcaaggagc
    13201 agcgctcgca cctggtcaag gagcacctgt gggtgctgtg gaccgactac ttcaaggccc
    13261 cgcacttcga gaagtacccc cagttgcaca ccctcatcaa cgacgccacg aagctggccg
    13321 gcgcgggtgg cacgaagggg tcgtgggacc tggccaaggc cgacgagttg ctggccaaga
    13381 tcgacgagat cgccgagatc ttctgggaga ccaagaaggc ctgaccgggc gcgccgcgag
    13441 ggcggcatac ccgatcgcga gggggcccga cggcctggtg ccgtcagggc cctttcgtat
    13501 gccgtctggc cagcacccgc acgagggagt cgacgacgcg ggggtcgtac tcatacccca
    13561 ggccgaggtg gatccgttcg acggcggcct cgcgccggcg caacgcgatc gagccaccca
    13621 cgagatcgtc gtaggcgttg gcgaccttca cgatgcggct gagcatcggc acctgctggc
    13681 gctgctcgcg cacgacccgg tagggcgtcg cctggtgctc gacggcgatg gccacctcgg
    13741 ggggcagacc ggtcttgcgc acgatgtcgg cgctgtcaga agcgatccgg cgttggtcgg
    13801 caggggccgc catgagggtg gcgccggcgg ggatgggggc gcgcagtgcc acctggccga
    13861 tgtcgtgcag caacgcggcg ttctcgaggt tgcggatatc gcgaccgctc aggccgagat
    13921 cgcggccgac ggccacgcac agttcggcca cccggtcggg gtgcccggct cgggtgaagc
    13981 cgcccatctc ggtgagccgc gagagcactc ggacggtctg ggcgtaggtc gcgcggatcg
    14041 cggcatactg ccgcagcgcg aactgagtga ggaccaacgg gacgaggaag agcggaaggg
    14101 ccaggacccc gagcgggcgt tcggccagcg cgatcagtgc ccccgtggcg ctcagggcca
    14161 gacccagccc agcgcccgag cggacctcat cgaggaaggc ctggcggagg ctgccagccg
    14221 aacgtgaagc catgacgagc gccgacagcc caaggaaggt gaacaggccg atcgccgcga
    14281 tgacggccag cagcaccgcg accggccagc ggcttcccac ccacgtggcg tcgacctcaa
    14341 gcagggtccg gcccgaccac aggggcgccc accggaacaa caatgcggtg acgaagatcg
    14401 cgaggaaccg cgccgccatc tcctccaccc gcaaggaccg accccgcgcg gcgtgcggca
    14461 gcgccccgca gagcatggcg atggcactga ccatgacgat cggcccaggg gcgaggtgcg
    14521 tgagatcgac ctgccaggac accgaggtga gggcgaacgc cagccccgtc gccagcgtca
    14581 tcggagccag ttgccgaccg tcctcgaccg ggaaccggaa gtactcgcca accccgatga
    14641 ggacggcgaa ggacgacacg accaaccacg cgctgaggaa cggcccgggg ttgcggatcg
    14701 tgttgatcgt cccgatcacc gcgaccgcca cggcgacatg caccacccat cgagcagtga
    14761 gccgacgccg aacgccgtgg gccggcccgc gaacgacccc gagggcgatg tcgggcgcgt
    14821 tcacgagccc tggcctgcgg tcgcggggcg gtcgatcccg gccaacatgt cggacatcac
    14881 gggatcgtcg tggtcccaca acggcgcgcc cgcgccttcg ggcggtggct cggcgacggc
    14941 ccaggtatgc cgctcaagga ctcgggcgag gacggccacc accgtcgggt ccaggtgggt
    15001 tccggcgcgt gactcgacga cctgaagggc acggttcgac ggcagtgcct ggcgcccggg
    15061 acgcccacgc gtgagcgagt cgaaactgtc ggccaccgca atgatccgcg aggccagtgg
    15121 gatgtccgtt ccaaccaact gatcggggta accacggccg tcccagcgct cgtggtgatg
    15181 cttgatgctg tcgagcgaat cctgcaggaa ggcgatgtcg ctcagcaggg ctacgccgac
    15241 ggcagggtgt cgggcgatga gttcgctcag ggatttgtcg ggtgccttgg tatccgagcc
    15301 ggcccaggcc tgagctccga gccacccgat gtcgtgcagg atcgcggcat actgcagtgc
    15361 ctctgtttgt gccgggccga ggccaagccc ctctcccaac agcacggata ggcgagcgac
    15421 ccgctcgctg tgcccgacgg catacgggtc ccgggtctcg ccggccgcga cgagggcggc
    15481 cagcgtgcgt tcgtgggcgc gctcctcctc ggcatattgg acgaaggccc actgggcgac
    15541 gaacaacggc agcaggatga ggaccgcgct gaacggtccg acctgcgcgg ggacccagag
    15601 gatgacgaag agcagaccga tgacgccgta gcccatctgg gccaggcccg aggtcgtcaa
    15661 catcgccagg aacatcgact ggaaggactc gccgctgtcg acccgaacga tcatcgcgat
    15721 gctggcgaag ttgaccaggc tgaagaccag gctcgccacg agaaacggac cccccacccg
    15781 gaccatcaac tcccccggac cggagattgt cgtgaggtcg gcggggccac cggcgagcag
    15841 ataggtgagc ccaccaaccg aaccgaggat ggcacccatc gaggcgttga acagacgagc
    15901 ccgccagccc gcagccccgc gttcgagcag gatcgtcgtg gccatcatca gggcgctgcc
    15961 caccgggcct acgaggacgc aggaggcgag agagacgatc ccactggctg ccaccgcgac
    16021 ccgctcgacc acccgcaccc gcacgacgcg agtgcctagg gtcagaacgg tgaggacggc
    16081 caacgaaaca tagtcatggg gaacgccgtc gagagcccag gccagtgtcg tgatgagaac
    16141 cgcgacgaca agcgtggcca ggatcacggc gttgacgcgc cgggcccgtg gacctgggtc
    16201 ggccgcggcc tgcggactca tggccccacg ctactgggaa gagtgtccca gagcccggcg
    16261 cggcccacga gcgtgctcac cgcgccggcc ggcggtgaca gacggggcga agcggtcagc
    16321 cgcgcttggg cggttcccag gtgaacatcg ccagcgggcc gtcgatcttg cccttgggct
    16381 cccacgtgaa catcccggtc ggaccatcga cacgcgtcgc cgcgcggcca ccccacaccg
    16441 gggtcgtctc cttggtcagc atggcttcga acaacttaga cacggtcttc atgaggtact
    16501 ccttgagatc tgggggaagc aggaactgga agagaggggg gtgcgcagga tcgccggcgg
    16561 gtcagccgcg cttgggcggt tcccaggtga acatcgcgag cgggccgtcg atcttgccct
    16621 tgggctccca cgtgaacatc ccggtcggac catcgacacg cgtcgccgcg cggccacccc
    16681 acaccggggt cgtctccttg gtcagcatgg cttcgaacaa cttggagaca gtcttcatgt
    16741 cagttccttt gactttcggt ggtattgaaa aggggtatcg ctggtcggca tccgcatcgc
    16801 caggtgacgg caggcgtggt gactgcgcca tgggtgagca cggcgccgcg tcacgggagt
    16861 tgggtggacg caggtcccca caaccgagtg acgtcctggg tcaacatcgc cacaaacagg
    16921 cgttggatgg ccttcatacg atcctcgaag tccgggagag tcggctactg aagccgtaca
    16981 cgagcctgag gtgtactcgt ctgcccctta gtgtgcgcca cggaactcgg acattccagt
    17041 cgaaaccgcg gacgcagcct cggccggaac cgctatcatt cgcgggctac accaaatacc
    17101 gaagaccgca tcgcaaacac cctggagtca ccatgagcaa gcgttcccgc aagcggcgtt
    17161 cccgcaagag ccacgccgcc aaccacgggc gcaagcccaa cgcctgagcg gccgcacccg
    17221 cgcgcgacct agttggtcgt ctcgatccga atgacggtca ggcggctgac gatggtcgcg
    17281 cgcagcgccg tgggggcggg ctccggcgca cacgacctct tgatcatcaa cttgaccatg
    17341 tggtcgaggt cgtactgacg taggcactcg gcacacgcgt ccagatgtgc ccgcaccttg
    17401 gcgaagtcat tcgcctccat ctcgccgtcg aggaactcga aggctcgcag caacgcctct
    17461 gaacagtcca aagccgtggg accgccatgc ggatggtgat gtccagggtc gacggacccc
    17521 gaggcggact ccgagacgaa cgccccagcg ggcgtggccg cagggcccgc ggatcgcgga
    17581 cccaccgaac tggagccgag cggaccgggc tcacggtgct cgctggtcat catcgcccct
    17641 cggtctggac gtcggtacgg acgtagccgc gctcggcggc atactcggtc agcagatcgc
    17701 gcaattgccg gcgcccgcgg tgtagccgtg acatcacggt cccgatgggg gtctgcatga
    17761 tctcggcgat ctccttgtag gcatacccct caacgtcggc gaaatagacc gccaaccgga
    17821 actcctcggg gatcgccagc agggcgcgct tgacgtcgct gtcgggcaga tgatccaacg
    17881 ccacgtcttc agccgaccga ccgcccgccg aggagtgcga gtcggcccga tgcagctggt
    17941 agtcctcgat ctcggcagcg tccgactgca acggctcgcg ctgcttcttg cggtaagagt
    18001 tgatgtaggt gttggtgagg atgcggtaca tccacgcctt gaggttggtc cccggctggt
    18061 actggtggaa cgccgcccag gccttggcgt aggtctcctg cacgaggtcc tcggcgtcag
    18121 cggcattgcg cgcggtgcgc agcgcggccg agtagagctg gtcaagcagc ggcatggctt
    18181 cgcgctcgaa gcgcgcgcgc cgctcctcgg tggtctcagt ggccaggtcg agggcaggcg
    18241 cagcgtcagc gacgacggga aggtcggtct cgggcagcac ctcggtggac gaggcggcga
    18301 gcgagccggt ggcgtcggaa ggggtcgggg acgcgggatc ggtggtcatc gcgccccaca
    18361 gtaacggtcc cgtgtgactg ccggcggcaa ccgaacctct ggtggcgacc ggcatgacga
    18421 acacgacgat gctcctagct tcccgggggt ggttgcggat cgtgacgtgc aacgcccccg
    18481 acggcatcgt tcattcccag ttcactcccc cagcacctca gccgggtatg ccgcgcagca
    18541 cctcggtgat cgccgcaccg accgctgagg ccgagccccg cggg
//
LOCUS       DAOMZY010000017        18417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_2663_length_18417_cov_6.045638, whole genome shotgun sequence.
ACCESSION   DAOMZY010000017 DAOMZY010000000
VERSION     DAOMZY010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 18417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18417
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2663_length_18417_cov_6.045638"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..1028
                     /locus_tag="PLK64_02045"
     CDS             57..1028
                     /locus_tag="PLK64_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV78551.1"
                     /translation="MSRTYAVVSAVRVFERPHVVSSPEASAIGDLRHVDEGESTHLWG
                     IWEGDALVGVITAWLPLSDNTDTVWMELDVHPGHRRRGHGTRAIETVVGFARANDRTR
                     IVTEAHYPVDRADDHPYRLFAEHNGFRLGNTEMIRRLTLPVDPHLLARLGDGARAAYQ
                     GRYRVEAFTEVPRALWPSLCECMNRVGTDAPTGEIDWEPETLTPERYGGYMELDRATG
                     RVRLTAVAIDEASGEVVAFSELALPPTITRAQQWGTLVRADHRGHRLGMAVKVANLVA
                     LQELYPERVDVTTGNANDNEWMVSINEQLGFRPLELCPAFYRTLDPV"
     gene            1082..1384
                     /locus_tag="PLK64_02050"
     CDS             1082..1384
                     /locus_tag="PLK64_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HPV78552.1"
                     /translation="MLVAFSVAPSAPDHDGSYSRAIADVVRLVRASGLPQRTDSMFTT
                     IEGEWDECMAVVKGAVDLLLEHSPRVSLVLKADIRPGHTGELDGKVNRIEAVLSEG"
     gene            1497..>3142
                     /locus_tag="PLK64_02055"
     CDS             1497..>3142
                     /locus_tag="PLK64_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78553.1"
                     /translation="MTWAALRYRPAQALLLAVLTALVVGCAAFAPLYERALAQSLLRD
                     GLSRMPPAATAIELTTSREQGARVRAERVRDIFPTSLAGVYGPGVERWSGRVFVTGSQ
                     GVSTVTMWGSPTPCDGLVLDAGRCPQAPFEVLASTAEARLQGWAPGAVISAVEARGIA
                     SGPAFASPVTVVGTYTQEELAAGPWAGLRLGGRAGTMSAGLAAAPLMDALVTSDGTFA
                     PDEQASSFVPGAPGWFQVAVSVTYPLNRDAISLESLDGIPGALSALGDEAARELPRIA
                     VSSAVGDLADELAEGRRQVLVIVPLLMAQLALLGVVVLGLVAESAVEQRRPEAALSRL
                     RGGGASGARRLLVAELGAAATVGAPLGLLGALGVTTLVRASWLAPGVPAELPWPTFAA
                     AGLGLILAWAAIIIAVGRVVREPVADLLRRVPSRQRGRRMGLVDAGVVGVSVAGVVAL
                     ASGNLAGPLALATPALVALAVGIVLAHLLTPAATLIGRRSLDRGRLTATLTAVQIARR
                     PAVRRIVTIITVAAALATFAVNALVVGQHNRELRARVETGA"
     assembly_gap    3143..3242
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3243..4379
                     /locus_tag="PLK64_02060"
     CDS             <3243..4379
                     /locus_tag="PLK64_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78554.1"
                     /translation="VRLGKPGGAEQTLSLDPIPLTSSVPVVFTAEVDCPQGCSLLAIG
                     VDRVGLDYRVLRGRFTITELRADAGPPAAIGRPDTWIGSGDKNADVLAPYAVPGDLGS
                     PTAVALDLLSARTGVRVTAKGAVGNLPAFVVGSLPPGTTSGGTFQGAGLDGIAVQFQP
                     VGTLPYTPGGPSNVAIVSLRALLDRASTLAPVVGRLQVYLADSSFEAPVRDALGAQGI
                     SVTTTVRESDRQALYDGSASAWGLRLAVVVGLLAIVMAALVLVLVVTTAWRPRSRDYA
                     ALRMSGVPLAVLRRASALEQGTVVVVAGVAGILCGLLASRLALPMVPLFTRPSATFLA
                     DLRPAWSAVGGAAALVLGLLGIVAIVVGTRLVSRAIPARLRERL"
     gene            4376..5236
                     /locus_tag="PLK64_02065"
     CDS             4376..5236
                     /locus_tag="PLK64_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPV78555.1"
                     /translation="MSAPTRSGLAITTHGLVHIYHADGHDLAALSGVDLRVASGEIVG
                     LLGPSGAGKSTLLTLCGGLLRPSAGTIRIGPNDLAQMDESALDRFRATEVGIVLQGAD
                     RNVLPYVTARRNVDFAQRGARQHGREVPDASEVLDLVGILPLANAPLATLTPGQLQLA
                     ALAVGIAAYPGVLLADEPTSQLDHAARDTVLAAIREVNAQVGTTVLIVTHDPQVAAAL
                     PRTVTIRDGRVGGEGRSGQEYAVVSADGSLPLPPDALDRLPPGTLVRVHPESDGRWII
                     IPEEDGHGPA"
     gene            5223..5939
                     /locus_tag="PLK64_02070"
     CDS             5223..5939
                     /locus_tag="PLK64_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPV78556.1"
                     /translation="MGRHELTGAERGLSSASRVERGPAQSVECRDVTVRFEPVTALAG
                     VSLRVQPGEFVAVTGPSGAGKSTLLWTLAGAVRPSEGQVTVGSTAIVSRDQAARLGVA
                     VVPQGNGLAAVLTALENVTLPLLAAGHGSAAARAAAADALAEVGLAESVGHLVEELSG
                     GQQQRVAVARGLALAGGVLLADEPTSELDHGNREIVLTLLRAYAAAGNVVVMATHDPE
                     AAAVADRVVRLDEGLLSVER"
     gene            complement(5949..6944)
                     /locus_tag="PLK64_02075"
     CDS             complement(5949..6944)
                     /locus_tag="PLK64_02075"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPV78557.1"
                     /translation="MEQRRWERLGRDLGVVGLGCWQLGAGWGAVSEDDAMATLHAAAD
                     AGVTCFDTADVYGDGRSERLVGRMRAERIADEEEPVFIATKMGRRANPHVPEAFTLEA
                     FRAWVDRSRANLDMDTLDLVQLHCPPPSVLDSDQIFDALDELVQEGRIAAYGVSVETC
                     AEALAAISRPGVVSVQIILNAFRPKPLEEVLPAAAAAGVAIIARVPLASGLLTGRYDE
                     STTFPAEDHRAFNRHGDAFDVGETFSGVPYEVGVQAAREIAALTPPGWSTAQLALRWV
                     LDQPGVSVVIPGARNPAQALDNVVAAHLDPLPQATLTAMAEIYDHHIREHIHARW"
     gene            7025..7558
                     /locus_tag="PLK64_02080"
     CDS             7025..7558
                     /locus_tag="PLK64_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPV78558.1"
                     /translation="MPLAFDPIRRAASLWRARWGPDAQVSAMAAATSIMRVHQALLAE
                     FDGIVGRHGLTFARYEALVLLAFTRSGELPMSKVGERLMVHPTSATNIVQRLEAVGLV
                     ERRPNPSDGRGTLAAITEPGRAVMEAATTDLVEAGFGLSMLDDADRDQLFGVLRRVRL
                     AKGDFLDGETEARSAES"
     gene            7635..9377
                     /locus_tag="PLK64_02085"
     CDS             7635..9377
                     /locus_tag="PLK64_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPV78559.1"
                     /translation="MEQTSGADRWQARYDTATARGQVRDADFTTLSGMPVEPVYGPGL
                     HGIPEPQTEAERERMERIGWPGEFPFTRGLYPTGYRGRTWTIRQFAGFGNAVQTNERY
                     KMILERGGGGLSVAFDMPTLMGRDSDDPHSLGEVGHCGVAIDSAADMEVLFGGIPLGD
                     VTTSMTISGPAVPVFCMYLVAAERQGVDIGVLNGTLQTDIFKEYIAQKEWLFEPGPHL
                     RLIGDLMEFCTERIPDYKPLSVSGYHIREAGSTAAQELAFTLADGFGYVELGLSRGLD
                     VETFAPGLSFFFDAHVDFFEEIAKFRAARRIWARWMRDVYGAKTDKAQWLRFHTQTAG
                     VSLTAQQPYNNVVRTAVEALAAVLGGTNSLHTNALDETLALPTEQAAEIALRTQQVIM
                     HETGVTNVADPLGGSWYVEALTDRMEAEAEAIFTKIKDLGVLGRRPGPSGQIEHEIGP
                     ITSGLLRGIEEGWFMSEIADAAFQYQISLEKGDKKVVGVNLLTESVSHDLEILRVSHQ
                     VEVDQVADLTARRSRRDEAAVQAGLAAMLAAARGGVNMIPAMLDAVRAEATLGEICDV
                     LRDEWGIYREPAQF"
     gene            9465..10532
                     /locus_tag="PLK64_02090"
     CDS             9465..10532
                     /locus_tag="PLK64_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78560.1"
                     /translation="MTLHHRSRAANLAALAVTGIALSVAVSACSSSTPSTASATSKAT
                     ARATATAKPSESTKPASGAVGAETPEQANAEVAKAITAQIAAAAVPGTKGKALREAAY
                     VDQALAAATAQGKLVSTLTAVQKADLALSPTDPVVLAVSRSAAYPRVILAKATFAGSG
                     SPALVTLVTPGADTAYRIASIARMLPSATVGKFDAVTAGSAPIGDASGLAVAPDALLA
                     AYSAALAYPAPAAAEGPFTEDAFFAAVRKGAADQAASLGSAVTLKQTHAPAGVVAALR
                     AATGQGAYVVAVIERKDSLTEKTANALTPSKAFTILSGKSVINKSAVLSTYEFVVFHI
                     PASGKATVVAAAEQLYAASGT"
     gene            10554..11597
                     /locus_tag="PLK64_02095"
     CDS             10554..11597
                     /locus_tag="PLK64_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPV78561.1"
                     /translation="MSSSPFTAAALRGAVDLSAFKRPANGPGARPGGAAGAGSAGSAG
                     SAGAGGAAGGSGAPGASASSGGDLVVSVNDATFQDIATTSLRVPIVVVLWSARLAQTA
                     SFVGVMADVARDNGGRFQVASVDVDTSPGLLRAFQIQSVPATIGLVQGQPVPLFVGAL
                     PPSEIQPWIDELLKLAVQMGVTGRVSVGDDAPAAAEGADGVDSADAAEDELPPLIAAA
                     YDAIERGDLDAAIAAYEEALTQNPADTEAALGLGQVRLLKRTEGVDAVAARAAAAADP
                     LDVAAQTVVADLDVLGGHVPDAFARLVDTVRATAGDDRNAARQHLLGLFEVVGASDER
                     VVKARRALMSALF"
     gene            complement(11677..14166)
                     /locus_tag="PLK64_02100"
     CDS             complement(11677..14166)
                     /locus_tag="PLK64_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPV78562.1"
                     /translation="MDQVTTHVGHPGLTSAEVADRVRDGRTNDMPARSGRSTGEIVRA
                     NVLTRINAILGILLVIVLTTGKLINAAFGLLIIANSAIGIIQELRAKRTLDNLAVIGE
                     ARPTVRRDGVSRPVTRTDIVADDVIELGPGDQLIVDGDVLEAASLEVDESLLTGEADP
                     VVKGPGDQVLSGSFVVAGSGAYRATKVGREAYAARIVEEAAKFTLVGSELRDGINRIL
                     LVVSWLLVPVGLLTIWTQLFGLGQPWREGLTGMVAALVPMVPEGLVLMTSIAFAVGVI
                     RLGQRQCLVQELPAIEGLARVDVVCADKTGTLTESGMRLSEVRGFDGSGMPSGPAPAE
                     VTEILAQLTATEPNPNASLAAIAEAITAPSVIWTETHHAPFTSARKWSGSTFDGQGTW
                     VLGAPDVLAPEGSAAREAAEEIGASGLRVLLLAEGSLPVTDPAAPGTVTPAALVILEQ
                     RVRPDARSTLEFFAEQHVQVKVISGDNARSVGAVTTLLGVVGGSDPIDARTLSQVDPD
                     DADTQAGQPPALGFVGGHHDAPTTHLSEAFVDAVARHTVFGRVTPDQKRAMVLALKSR
                     GHTVAMTGDGVNDVLALKKADIGVAMGAGSPATRAVAQIVLLDNKFATLPYVVAEGRR
                     VIGNIERVANLFLTKTVYSVLIALLVGIWQLPFPLQPIHVTITGWFTIGVPAFLLSLA
                     PNHERARPGFARRVLQLALPSGVVVAICTFTTYLLCRPPVGSGEALRTQASTASLACL
                     IIVATWVLSVVARPYEWWKVALLVLSVAAYVAIFLVPFTQSLFLLDAGNAAMMRTGLT
                     VGLAGAATIEAIWWIQGRLRGEPRRWWVADD"
     gene            14237..14797
                     /locus_tag="PLK64_02105"
     CDS             14237..14797
                     /locus_tag="PLK64_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HPV78563.1"
                     /translation="MDVGARAQARRREGAGAVDLEGFVNAHYAQLLRRAYLLTGSRAD
                     AEDLVQEALARTWEAARGRAVESPGAYVHRTMVNLYVSRWRRRRLRREDPVAEIPEPV
                     AGGARHTADAVGEVDERDRLWRVLGSLPAKQRAVLVLRYYEDLSEGAAADLLGVTVGT
                     VRSQTWKGLRRLEAALGSRVEGDGRG"
     gene            14790..15587
                     /locus_tag="PLK64_02110"
     CDS             14790..15587
                     /locus_tag="PLK64_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78564.1"
                     /translation="MADLEAQVRATLADRAGRLEGPLPYAAPPALVDAVATRAAQLHR
                     RRTLVRVGGAAAAVAVIGLAAATLVRGPSVAGPLGPAGSVTATATATRTVDSATATAT
                     ATATRTVDSGTSGDTSAQAAAEDRIIRAALAADPAFPAGWDPQSQAVFGCGFYRVGAS
                     ADLTYRYVWANCSMFQGDTVVSGMSVPLWLHVRGSGESLEVLGHEIPGDGTAYTADVR
                     RLFPADLVDTFLGAPTTEIVRVAGALALRDAREQRASAAGTTPTAAP"
     gene            complement(15695..16672)
                     /locus_tag="PLK64_02115"
     CDS             complement(15695..16672)
                     /locus_tag="PLK64_02115"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HPV78565.1"
                     /translation="MREPQAAGSAALRDRRPARYAALDETVLRMLAAGDGVVSYHALR
                     GLGYSLNDVSSLVRQGLLVRLWHGRYGDGATSKTLDAGPRHLLTARAVLAGMPRHALS
                     HQTALLAWSLPVLRRDLDIVHVSGIDFDRSRRATGLWVHPAIDSAATTKRSGLHVASP
                     SVAVAQTAALVGPRAGLMAADAGLRSRLFTRDALAAAVASVPRPRQAARIVDLASAAS
                     ESAGESWCRLVFADLGLAQPRQQVEIFDEEGRFVARVDFLFEQARVVVEFDGAVKYSG
                     PEGREHLIAEKRREDALRRLGYRVVRLLWSDLSNPERVARLLSPLRATA"
     gene            complement(16952..17419)
                     /locus_tag="PLK64_02120"
     CDS             complement(16952..17419)
                     /locus_tag="PLK64_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HPV78566.1"
                     /translation="MTTAFSTRPGWHDWGVGIAYAVAQRADCTRRRVGAVVFDEANRI
                     LATGYNGAPAGRVGCLEGGCPRGSLGHDELPPDAPYEDPSSPGFCISIHAESNALLFA
                     DPVRRRGGWIAITHPPCPGCQKLLANSGLTTAIWRTDAGALVEWPIGTPMPPR"
     gene            complement(17416..>18417)
                     /locus_tag="PLK64_02125"
     CDS             complement(17416..>18417)
                     /locus_tag="PLK64_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase"
                     /protein_id="HPV78567.1"
                     /translation="LPNVVDIDAIKAAGIRIGADPLGGASVEFWGAMAERLGIDLTVI
                     NPLVDPTWRFMTLDWDGKIRMDCSSPYAMASLIANKDAFAISTGNDADSDRHGIVTPD
                     GGLMNPNHYLAVAIQYLYGGGRPGWRPDGFIGKTLVSSSMIDRVASDLGRRLVEVPVG
                     FKWFVPGLLDGSGPFGGEESAGASFARFDGSVWTTDKDGILLALLASEILARTGSSPS
                     EHYRELTAKHGAPAYARIDAPANREQKAKLGALSPADVAADSLAGEPITAKLTEAPGN
                     GAKIGGLKVVTESAWFAARPSGTEDVYKIYAESFRGAEHLAQVQAEARDVVSAALGG"
BASE COUNT         2623 a         6359 c         6572 g         2763 t
ORIGIN      
        1 gcacctacgg tgggcgggtg gccatcaccc ccatcgacct cgccgatgac gcgctcatgt
       61 cccgcacgta tgccgtggtc tcggcagtgc gcgtcttcga gcgaccccac gtcgtgagtt
      121 cacccgaagc gtccgcgatc ggcgatctgc ggcacgtcga cgagggggag agcacccacc
      181 tgtgggggat ctgggagggc gatgcgctcg tcggtgtcat cacggcgtgg ctgcccttga
      241 gcgacaacac cgacaccgtc tggatggagc tcgacgtgca tccgggccac cgccggcggg
      301 gccacggcac ccgggcgatc gagacggtag tcggcttcgc ccgagccaac gaccgcaccc
      361 gaatcgtcac cgaggcccac taccccgtcg accgtgccga cgaccacccg tatcgactct
      421 tcgccgagca caacggattc cggctcggca acaccgagat gatccgacgg ctcaccctgc
      481 cggtcgaccc gcacctgctg gcccggctcg gcgacggtgc gcgggcggca taccaggggc
      541 gttatcgggt ggaggcgttc acggaggtgc cgcgcgcgct gtggccctcg ctgtgcgagt
      601 gcatgaaccg ggtggggacc gatgccccga ccggcgagat cgactgggag cccgagacgc
      661 tgaccccgga gcgctacggg ggatacatgg aactggaccg cgcgacgggg cgggtgcggt
      721 tgaccgcagt cgccatcgac gaggcaagtg gcgaggtcgt ggccttcagc gagttggctc
      781 tcccgcccac gatcactcgg gcccaacagt ggggcacgct cgtgcgggcc gaccatcgcg
      841 gtcatcgtct gggcatggcc gtcaaggttg ccaatctcgt tgcactgcag gagctttacc
      901 ccgagcgtgt cgatgtcacg accggcaacg ccaacgacaa cgagtggatg gtgtcgatca
      961 acgaacagct ggggttccgg ccgctggagc tgtgcccggc gttctatcgg acgctcgacc
     1021 ccgtctgatc cggcccgctc caccagcccc acgggcgcgc gatgcggcat acggtggggg
     1081 catgctcgtc gccttctccg tcgccccgtc cgctcccgac cacgacggct cctacagccg
     1141 cgcgatcgcc gacgtcgtcc gactcgtgcg agcctccggg ctcccccaac gcaccgactc
     1201 gatgttcacc acgatcgagg gcgagtggga cgagtgcatg gccgtggtca agggagccgt
     1261 cgacctgctg ctggagcact caccgcgggt gagccttgtg ctcaaggcgg atatccgccc
     1321 cggtcacacc ggcgaactgg acggcaaggt caaccgcatc gaggccgtgc tctccgaagg
     1381 ctgacctgcc cctcagtgtc gcgccccacc ccggcccgcc gctgcgagcc cagaccggac
     1441 ggcataccgt ggtgggggag ccgtcaccag gcgaacgaga ggtcaggggg tgcgggatga
     1501 cgtgggctgc gttgcgctac cgtcccgcgc aagcccttct cctcgccgtg ctcaccgcgc
     1561 tggtggtggg ctgcgcggcg ttcgcgccgc tttacgaacg ggcgctcgct cagtcgctgc
     1621 tgcgcgacgg cctgtcccgt atgccgcccg cggcgaccgc gatcgagctc accacctcgc
     1681 gggagcaggg ggctcgggtg cgggccgaac gggtgcgcga catcttcccg acatcgctgg
     1741 ccggggtcta cggcccgggg gtcgagcgat ggtccggtcg ggtcttcgtc accggctcgc
     1801 agggcgtgtc gaccgtgacg atgtggggaa gtccgacgcc gtgcgacggg ctggtgctcg
     1861 acgctgggcg ttgcccgcag gcgccgttcg aggtgctggc ctcgaccgcc gaggcacgcc
     1921 tccaggggtg ggcccccggc gcggtgatct ctgccgtcga agcccgcggg attgccagcg
     1981 gccccgcctt cgccagcccg gtgacggtgg tcggcaccta tacgcaggag gaactggccg
     2041 caggcccctg ggcggggctt cggctcggtg ggcgggctgg gaccatgtcg gcgggtctgg
     2101 ctgcggcccc gctcatggat gcactcgtca ccagcgacgg caccttcgca ccggacgagc
     2161 aggcctcgtc gttcgtcccg ggcgcaccgg gatggttcca ggtcgccgtc agcgtgacct
     2221 acccgttgaa ccgtgacgcg atcagcctcg agagcctgga cggcatcccg ggcgcgctga
     2281 gcgctctggg ggatgaggca gcgcgagagc tacctcgaat cgccgtctcg agtgccgttg
     2341 gcgatctggc ggacgagttg gctgaaggtc gccgccaggt gctcgtcatc gtcccgttgc
     2401 tcatggccca acttgccctg ctcggtgtcg tcgtgctggg actggtggcg gagtctgcgg
     2461 tggagcagcg ccgtcccgag gcagcgttga gccgattgcg cggaggtggg gcttcgggag
     2521 cgcggcggtt gctcgtcgcc gaactcggtg ccgcggccac cgtcggtgcg cccctcggat
     2581 tgctcggcgc tctcggagtc accaccctcg ttcgagccag ctggctggcc cccggagtcc
     2641 cggccgaact gccctggccg acgttcgcgg ccgcgggttt gggcctgatc ctggcctggg
     2701 cggcgatcat catcgccgtc ggaagggtgg tccgcgagcc cgtggcggac ttgttgcgcc
     2761 gggtgcccag tcgccagcga ggccggcgga tgggtcttgt cgatgccggc gttgtcgggg
     2821 tgtcggtcgc cggggtggtg gcgctggcca gcggcaacct cgccggaccg ctcgccctcg
     2881 cgacccctgc cctggtcgcg ctggccgtgg ggatcgtgct ggcccacctg ctcaccccgg
     2941 ccgccaccct catcgggcga cgatcgctgg accgaggtcg gctcacggcg acgcttaccg
     3001 cggtccagat cgctcgacgt ccggccgtcc ggcggatcgt cacgatcatc accgtggcgg
     3061 cggcgctcgc gacctttgcg gtcaacgcac tcgtcgtggg gcagcacaac cgggaactgc
     3121 gcgcgcgggt ggagaccggg gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nngtccgact gggcaaaccc ggcggagccg agcagacgtt gagcctcgac ccgatcccct
     3301 tgacgagcag cgtgccggtc gtgttcacgg ccgaggtcga ctgtccccag gggtgctcgc
     3361 tgctggccat cggggtcgac cgggtggggt tggactatcg ggtcctgcgg ggccgattca
     3421 ccatcaccga actgcgcgcc gacgcgggtc cgccggcagc tatcggccgc cccgacacgt
     3481 ggatcggctc gggcgacaag aacgccgacg tgctcgctcc ttacgcggtc ccaggcgatc
     3541 tcggcagccc caccgcagtg gccctcgacc tgctcagcgc ccgcacgggc gtccgggtca
     3601 ccgccaaggg cgccgtcgga aaccttccgg cgttcgtcgt cggctcgttg cctccgggca
     3661 ccacctctgg agggacgttc cagggggctg gcctggacgg gatcgccgtg cagttccagc
     3721 cggtgggcac gctgccctac accccgggcg gcccctccaa cgtcgcgatc gtcagcttgc
     3781 gcgcgctgct cgaccgggcc agcacccttg cgcccgtcgt cggtcggctg caggtctatc
     3841 tcgccgactc cagctttgag gcgccggtgc gcgatgccct tggcgcccaa gggatctcgg
     3901 tcaccaccac ggtgcgagag agtgaccggc aggcgctcta cgacgggtcg gcgtcggcgt
     3961 ggggcctgcg tctggccgtc gtcgtcggac tgcttgccat cgtcatggcc gccctcgtcc
     4021 tcgtcctggt cgtgaccacg gcctggcgcc cgcgctcgcg ggactacgcc gccctgcgaa
     4081 tgtccggcgt gccgcttgcc gtgctccgtc gtgcaagcgc cctcgaacag gggacggtcg
     4141 tcgtggtcgc cggggtggcc ggcatactct gcggactcct ggcgtcgcgc ctggccctgc
     4201 cgatggtgcc gttgttcacc cggccttcgg cgaccttcct ggccgacctg cgcccggcgt
     4261 ggtccgccgt cggcggggcc gcggcgctcg tcctcgggct gctggggatc gtcgccatcg
     4321 tcgtgggcac tcgcttggtt tctcgagcca tcccagcccg attgagggaa cgcctgtgag
     4381 cgccccgacc cgctccggcc tggccatcac gacccacggc ctcgttcaca tctaccacgc
     4441 ggacgggcac gacctcgctg ccctctccgg cgtcgacctg cgggtcgcgt caggagagat
     4501 cgtcgggttg ctcggtccgt cgggcgccgg gaagtcgacc ctgctcaccc tgtgcggcgg
     4561 gctgctgcgc cccagcgccg ggaccatccg gatcggcccg aacgacctgg cccagatgga
     4621 tgaatccgcg ctggaccgct tccgggccac tgaggtcggg atcgtcttgc agggggccga
     4681 ccgcaacgtg ctgccctatg tcacggcgcg tcggaacgtc gacttcgctc agcgcggagc
     4741 gcgacagcat ggccgcgagg tgcccgatgc cagcgaggtc ctcgacttgg tcggcatcct
     4801 cccgctggcc aatgccccgc tcgccacgct cactcccggg cagttgcagc tcgcggcgct
     4861 cgcggtgggg atcgcggcat acccgggggt gctccttgcc gacgagccga ccagccaact
     4921 ggaccacgcc gcccgcgaca ccgtgctggc cgcgatccgc gaggtgaacg cccaggtagg
     4981 gaccaccgtc ctcatcgtca cccatgaccc gcaggtggcg gccgcgctgc cgcgcacggt
     5041 gacgatccgc gacggtcggg tcggcgggga gggacgcagc ggccaggagt atgccgtggt
     5101 gtcggcggac gggtcgttgc cgctgccgcc cgacgccctc gacagactgc cgccgggcac
     5161 ccttgtgcgc gtccatccgg agtctgacgg ccgctggatc atcatcccgg aggaggacgg
     5221 ccatgggccg gcatgagctg acgggcgccg aacgcgggct ttcgtcggcc agtcgagtgg
     5281 aacgcgggcc ggcgcagtcg gtggagtgcc gagacgtcac ggtccgcttc gagccggtca
     5341 ccgcgttggc gggagtgtcg ctgcgggtgc agccggggga gttcgttgcc gtcaccggtc
     5401 cgtcgggagc cgggaaatcg accctgctct ggaccctggc gggtgccgtg cgaccgagcg
     5461 agggccaggt gacagtgggg tcgacggcca tcgtgagccg cgaccaggct gcgcgtctcg
     5521 gagtggccgt cgtgccgcaa ggtaacggcc tggccgcggt gctcaccgct cttgagaatg
     5581 tcactctgcc gctgctcgcg gcgggccacg gatccgccgc ggcccgtgcc gccgctgctg
     5641 acgcactggc ggaggtcggt ttggccgagt cggtggggca cctcgtcgag gagttgtcag
     5701 gcggtcagca acagcgggtc gccgtggccc gcggactggc cctcgccgga ggagtgttgc
     5761 tcgccgatga gccgacgtcc gagctcgacc acggcaaccg cgagatcgtc ctgaccttgc
     5821 tgcgggcgta tgcggctgcc ggaaacgttg tcgtcatggc gacccacgac ccggaggcag
     5881 cggccgtcgc cgaccgggtc gtccgactcg acgaaggcct gctcagcgtc gaacggtgat
     5941 gcgtgtgctc accaacgggc gtggatgtgt tcgcggatgt ggtggtcgta gatctcggcc
     6001 atggccgtca gggtggcctg cggcaacggg tcgaggtggg cggcgacgac gttgtccaga
     6061 gcttgcgccg ggttgcgggc gcccgggatg accacggaca ctcccggctg atcgagcacc
     6121 cagcgcagcg cgagttgcgc cgtcgaccag ccgggtgggg tgagcgccgc gatctcgcga
     6181 gccgcctgga ccccgacctc gtagggcacg cctgagaagg tctcgccgac atcgaacgcg
     6241 tccccgtgtc ggttgaacgc gcggtggtcc tccgcgggga acgtggtcga ctcgtcgtaa
     6301 cgcccggtca gcagcccgct ggccagcggc acccgcgcga tgatcgcgac cccagcagcg
     6361 gccgccgccg gcaggacctc ctccagcggc ttgggccgga aggcgttgag gatgatttgc
     6421 acactcacca cgccgggtcg cgagatcgcg gcgagggctt ccgcgcaggt ctcgaccgac
     6481 actccgtatg ccgcgatccg cccctcctgc acgagctcgt cgagagcgtc gaaaatctgg
     6541 tcgctgtcca ggacgctcgg cggggggcag tgcaactgga cgaggtcgag ggtgtccatg
     6601 tcgaggttgg cccgactacg gtcgacccat gcgcggaaag cctccagcgt gaacgcttcc
     6661 ggcacatggg gattggcacg tcgacccatc ttggtcgcga tgaagaccgg ctcctcctcg
     6721 tcggcgatcc gctcggcgcg catccggccg accaatcgct ccgagcgccc gtcgccatag
     6781 acatcggcgg tgtcgaagca ggtcactccc gcatcggctg ccgcgtgcag agtcgccatg
     6841 gcgtcgtcct cgctgaccgc gccccagccc gcgcccaact gccagcagcc caacccgaca
     6901 acgccgaggt cgcgtcctag ccgttcccac cgtcgctgtt ccatgggcgc caacggtatc
     6961 cccgcggcat accaggtggg gggcggcata cctgccgggt ggcggagcgc gctagcctgc
     7021 ggccgtgccc ttggccttcg atcccatccg ccgcgccgcg tcgttgtggc gcgctcgatg
     7081 ggggccggac gcccaggtga gcgcgatggc ggcggcgacc tcgatcatgc gggtgcacca
     7141 ggcgctgctc gccgagttcg acgggatcgt cgggcggcac gggctgacct tcgcgcgcta
     7201 cgaggcgttg gtgctgctgg ccttcacgcg ttctggtgaa ctgccgatga gcaaggtcgg
     7261 tgagcgcctc atggtccacc cgacctcggc gaccaacatc gtccaacggc tggaggcagt
     7321 cgggctggtc gagcggcgcc ccaaccccag cgacgggcga ggcaccctgg cggcgatcac
     7381 cgagccgggc cgcgccgtca tggaggctgc gaccacagac ctcgtcgagg ccggcttcgg
     7441 gctgagcatg ctcgacgacg ccgatcgtga ccagctcttc ggggtgctgc gtcgggtccg
     7501 gctcgccaag ggcgacttcc tcgacggcga gaccgaggcc agatccgctg agagctgagt
     7561 gcggtgccgt gacggacgtc gcaaagtagt aggacgtcct agtaaatctg ccatttcccg
     7621 gttaccctgt ccgcatggag cagacctcag gcgcggaccg ctggcaagcc cgctacgaca
     7681 cggcaacggc gcgcggtcag gtgcgcgacg cggacttcac caccttgtcc ggtatgccgg
     7741 tcgagccggt ctacgggccg gggctgcacg gcatacccga gccgcagacc gaggctgagc
     7801 gggagcggat ggagcggatc gggtggccgg gggagttccc cttcacccgc ggtctctacc
     7861 ccacgggcta tcgcgggcgg acgtggacga tccggcagtt cgccgggttc gggaacgcgg
     7921 tccagaccaa cgagcgctac aagatgatcc ttgagcgtgg cggcggcggg ctctcggtcg
     7981 ccttcgacat gccgaccctc atgggccgcg actccgacga cccgcacagc ctcggtgagg
     8041 tcgggcactg cggtgtcgcc atcgactcgg cggccgacat ggaggtgttg ttcggcggca
     8101 tacccctggg tgacgtgacc acctcgatga cgatcagcgg gcccgcggtg ccggtcttct
     8161 gcatgtacct cgtcgcggcc gagcggcagg gcgtggacat cggcgtcctc aatgggaccc
     8221 tgcagaccga catcttcaag gaatacatcg cccagaagga atggctcttc gagcccggcc
     8281 cgcacctgcg gctcatcggc gatctcatgg agttctgcac cgagcggatc ccggactaca
     8341 agccgctgtc ggtgtcgggc tatcacattc gcgaggccgg ctcgacggcc gcgcaggagt
     8401 tggcgttcac cctcgccgat ggtttcggtt acgtcgagtt ggggctctcg cgcgggctgg
     8461 acgtcgagac cttcgcgccg gggctgtcgt tcttcttcga cgcccatgtc gacttcttcg
     8521 aggagatcgc gaaattccgt gcggcacgcc ggatctgggc ccgctggatg cgcgacgtct
     8581 acggcgccaa gaccgacaag gcgcagtggc tgcggttcca cacccagacc gcgggagtct
     8641 cgctcaccgc gcagcagccc tacaacaacg tcgtccgcac tgcggtcgag gcgctcgccg
     8701 cagtgctcgg cggcaccaac tccctgcaca ccaatgccct cgacgagacc ctcgccctgc
     8761 cgaccgagca ggccgccgag atcgcgttgc gcacccagca ggtgatcatg cacgagaccg
     8821 gtgtgaccaa cgttgccgac ccgctcggtg gctcctggta tgtcgaggcc ctcaccgacc
     8881 ggatggaggc cgaggccgag gcgatcttca cgaagatcaa ggacctcggc gtgctgggcc
     8941 gccggcccgg accgtccggc cagatcgagc acgagatcgg cccgatcacc tccggcctgc
     9001 tgcgtggcat cgaggagggc tggttcatgt cggagatcgc cgacgcggcc ttccaatacc
     9061 aaatctcgct cgagaagggc gacaagaagg tcgtcggcgt caacctcctg accgagtcgg
     9121 tgagccacga cctggagatc ctgcgggtca gccaccaggt cgaggtggac caggttgccg
     9181 acctgaccgc tcgccgctca cgccgcgacg aggccgcggt ccaggccggg ctggcggcga
     9241 tgctcgcggc ggctcgcggc ggcgtcaaca tgattcccgc gatgctcgac gcggtccgcg
     9301 ccgaggccac gctcggcgag atctgcgacg tgctgcgcga cgagtggggc atctatcggg
     9361 agcccgcaca gttctgagcg ggggtgcgcg cggggaccga tttggtccct cacgggttgc
     9421 tgcgcctacc ctggacgaac cccaccggga ggatcacacc ccacatgacg ctccaccatc
     9481 gctctcgtgc cgccaacctg gccgcgctcg ccgtgaccgg gatcgctctg tccgtagctg
     9541 tctccgcgtg ctcgtcatcg accccgtcga cggcgtcggc gaccagcaag gcgactgcgc
     9601 gcgcgacggc gaccgcgaag ccgagcgagt cgacgaagcc tgccagcggt gcggtcggcg
     9661 ccgagacgcc ggagcaggcc aacgcggagg tcgccaaggc aatcaccgct cagatcgccg
     9721 cggctgccgt gccggggacc aaggggaagg ccctgcgcga agccgcctat gtcgaccagg
     9781 ccctggcggc ggccaccgca cagggcaagc tcgtgagcac tctgacggct gtccagaagg
     9841 ccgatctcgc gctgagcccc acggacccgg tcgtcctggc ggtgtcgcgc agcgcggcat
     9901 accccagggt gatcctcgcc aaggcgacct tcgccgggag cgggtcaccc gccctcgtga
     9961 cgctcgtgac gcccggcgcc gacaccgcct atcggatcgc ctcgatcgcg cggatgctgc
    10021 cgtcggcaac ggtcggcaag ttcgatgcgg tgaccgccgg ctcggccccg atcggggacg
    10081 cttcgggtct ggccgtcgcc ccggatgccc tgctcgcggc atactcggcg gccctcgcct
    10141 atcccgcgcc tgccgcagcc gaggggccgt tcacggagga cgccttcttc gcggcggtcc
    10201 gcaagggggc cgctgaccag gccgcctcgc tgggctcggc cgtcaccctc aagcagaccc
    10261 atgcccccgc gggggtcgtc gctgcgctgc gcgcggccac gggccagggc gcctacgtcg
    10321 tcgccgtgat cgaacgcaag gactcactga ccgagaagac cgccaacgcg ctgacgccgt
    10381 cgaaggcctt cacgatcctg tccggcaagt cggtcatcaa caagagcgcc gtgctgagca
    10441 cctacgagtt cgtggtcttc cacatccccg ccagcggcaa ggccactgtc gtcgccgcgg
    10501 cggagcaact gtatgccgcg tccggcacct gagcgatcgg cgagaatgac ctcatgagct
    10561 cgtctccctt cactgccgct gccctgcgcg gggccgtcga cctgtccgcc ttcaaacgcc
    10621 cggccaacgg cccgggagcc cgcccgggtg gggcagccgg agcaggttcg gccggctcgg
    10681 ccggctcggc cggggctggt ggcgctgccg ggggctctgg cgcgcctggc gcgtcggcca
    10741 gctcgggcgg cgacctggtc gtctcggtca acgatgcgac cttccaggac atcgcgacga
    10801 ccagccttcg ggtgccgatc gtcgtcgtgc tctggtctgc gcggctggct cagacggcct
    10861 ccttcgtcgg cgtcatggcc gacgtcgccc gcgacaacgg gggccgattc caggtggcgt
    10921 cggtcgacgt ggacaccagc cccgggctgc tgcgggcctt ccagatccag tcggtgccgg
    10981 ccaccatcgg cctcgtccaa gggcagccgg tgccgctgtt cgtcggagcg ctaccgccgt
    11041 cggagatcca gccgtggatc gacgagctgc tcaagttggc cgtgcagatg ggcgtcacgg
    11101 gtcgggtctc ggtcggggac gacgcgccgg cagccgccga gggcgccgac ggcgtcgaca
    11161 gtgctgatgc tgccgaggac gagctgccgc ccctgatcgc agcggcctac gacgcgatcg
    11221 agcgtggcga cctggacgcg gcgatcgcgg catacgagga agcgctcacc cagaaccccg
    11281 ccgacacgga ggccgcgctg ggcctcggcc aggtccggct gctcaagcgc accgagggcg
    11341 tcgacgcggt tgcggcccgg gcggcggccg cggccgaccc gctcgacgtg gcggcgcaga
    11401 cggtcgtcgc cgacctcgat gtcctcgggg ggcacgtgcc tgacgcgttc gcccggttgg
    11461 ttgacacggt tcgggcgacg gcgggcgacg atcgcaacgc ggcgcgccag cacctgctcg
    11521 ggctcttcga ggtggtcggg gcgagtgacg agcgggtcgt caaggcccga cgcgcgttga
    11581 tgagcgcgct gttctgacgc ttggcgtgcg agtcggctga cggcgcggct ggcgggcagg
    11641 cgggttgacg ggttgaccgc cggtggtgcg agtcggtcag tcgtcggcga cccaccagcg
    11701 gcgcggttcg ccgcgcaacc ggccctggat ccaccagatc gcttcgatgg tggccgctcc
    11761 tgccagcccg actgtcagcc cggtgcgcat catcgcggcg ttcccggcgt cgagcaggaa
    11821 cagtgactgc gtgaacggca cgaggaagat cgcgacgtag gccgccaccg acagcaccag
    11881 cagggccacc ttccaccact cgtagggccg ggcgacgacc gacagcaccc aggtcgccac
    11941 gatgatgagg caggccaagg acgccgtcga ggcctgggtg cgaagagcct cgccggaccc
    12001 gaccggcggt cggcacagca ggtaggtcgt gaaggtgcag atggccacga cgacgccgga
    12061 gggaagggcc agttgcagga cccgtcgggc gaaccccggc cgggcccgct cgtggttggg
    12121 cgccagcgac agcaggaacg ccggcactcc gatggtgaac cacccggtga tcgtcacgtg
    12181 gatgggctgc agggggaacg gcagttgcca gatcccgacg agcagcgcga tcagcactga
    12241 gtagacggtc ttggtgagga acaggttggc gacccgttcg atgttgccga tgacccggcg
    12301 cccctcggcg acgacgtagg ggagggtcgc aaacttgttg tccagcaaca cgatctgcgc
    12361 gacggcccga gtcgcaggcg atcccgcgcc catcgcgacc ccgatgtcgg ccttcttcag
    12421 ggcgaggacg tcgttgaccc cgtcaccggt catcgcgacc gtgtgtcctc ggctcttgag
    12481 cgccaagacc atcgcgcgtt tctgatcggg ggtgacccgt ccgaacacgg tatgccgtgc
    12541 cacggcgtcg acgaaggcct ccgacaggtg cgtggtcggg gcgtcatggt ggccgccgac
    12601 gaacccgagc gccggcggct ggcccgcctg ggtgtcggcg tcgtcggggt cgacctgcga
    12661 gagggtccgg gcgtcgatcg ggtcgctgcc tccgactact ccgagcaggg tcgtcaccgc
    12721 gccgacggat cgagcgttgt cgcccgagat caccttgacc tgcacgtgct gctcggcgaa
    12781 gaactccagg gtgcttcggg cgtcgggccg gacccgctgc tcgaggatga cgagcgccgc
    12841 aggggtcacg gtgccgggtg ccgcggggtc ggtgacgggc agcgacccct cggccagcag
    12901 cagcacccgc aacccgctcg cgccgatctc ctcggcggcc tcgcgagccg ccgacccttc
    12961 cggcgccagg acgtcagggg caccgagcac ccaggtgccc tgcccgtcga acgtcgaccc
    13021 cgaccatttg cgggcggagg tgaagggggc atggtgggtc tcggtccaga tgaccgacgg
    13081 cgcggtgatg gcctcggcga tggcggccag gctcgcgttc gggttgggct ccgtggctgt
    13141 gagctgggcg aggatctcgg tgacttcggc tggggccggg ccggacggca taccggagcc
    13201 gtcgaaaccg cgcacctcgg acaggcgcat ccccgactcg gtgagggtcc cggtcttgtc
    13261 ggcgcagacg acgtcgacgc gagccaggcc ctcgatggcc ggcaactctt ggacgaggca
    13321 ctggcgctgc ccgagccgga tgaccccgac cgcgaaggca atcgacgtca tgagcacgag
    13381 gccttcgggg accatcggga ccagcgcggc gaccatgccc gtcaggccct cgcgccacgg
    13441 ttgcccgagg ccgaacaact gggtccagat cgtcagcagg ccgaccggga cgagcaacca
    13501 cgagacgacg aggaggatcc ggttgatccc gtcgcgcaac tcggacccga cgagggtgaa
    13561 cttggcggcc tcctcgacga tccgcgcggc atacgcctcg cggccgacct tcgtcgcgcg
    13621 gtaggcgccg gaaccggcga cgacgaaact gcccgagagc acctgatcgc cgggcccctt
    13681 gacgaccggg tcggcctcgc cagtgagcag cgactcgtcg acctccaggc tggccgcttc
    13741 gagcacgtcg ccatcgacga tgagttggtc gccggggccg agctcgatga cgtcgtcggc
    13801 gacgatgtcg gtgcgagtga ccgggcggct caccccgtcg cgccggacgg tcgggcgggc
    13861 ctcgccgatg accgcgaggt tgtccagggt gcgcttggcg cgcaactcct ggatgatccc
    13921 gatcgcgctg ttggcgatga tgagcagccc gaaggcggcg ttgatgagtt tgccggtcgt
    13981 gaggacgatg accagcagga tgccgaggat ggcgttgatc cgggtgagga cgttggcccg
    14041 gacgatctcg ccagtcgagc gtccgctgcg ggcgggcatg tcattcgtgc gcccgtcgcg
    14101 cacccgatcc gccacttctg cgctggtcag gccgggatga ccaacgtgcg tcgtgacctg
    14161 gtccatggtt gggatcctag gtgagtgggg cggcggcata gttctgcgac cctggtgcgt
    14221 cgaacctgat gtgacgatgg atgtgggtgc gcgggcgcag gctcgtcggc gagagggggc
    14281 gggggcggtg gacctcgaag gtttcgtcaa cgcccactac gcccagttgc tgcggcgggc
    14341 ctatctgctg accggatcac gcgcggacgc cgaggacctg gtgcaggagg cgttggcccg
    14401 gacctgggag gccgcgcggg gacgggcggt cgagtcgccg ggggcctacg tccaccggac
    14461 gatggtcaac ctctatgtca gtcgctggcg ccggcgccgg ttgcgccgcg aggatccggt
    14521 ggccgagatc cccgagccgg tggctggcgg cgcgcggcat accgcggacg cggtgggcga
    14581 ggtcgacgag cgggatcggc tgtggcgggt gttggggtcg ctgccggcca agcaacgtgc
    14641 ggtgctcgtg ctgcgctatt acgaggacct cagcgagggc gcggccgccg acctgctcgg
    14701 ggtcaccgtc ggaaccgttc gcagtcagac ctggaagggc ctgcgccggc tggaggccgc
    14761 gctcggctct cgcgtggaag gagacggccg tggctgacct cgaggcgcag gtgcgggcga
    14821 ccctcgccga ccgagcgggt cggctggagg gaccgctccc gtatgccgct ccgccggctc
    14881 tcgtcgacgc cgtggcgacc cgggctgcgc agttgcaccg gcggcgcacc cttgtgcggg
    14941 tgggcggtgc ggctgctgcg gtggccgtca tcgggctcgc ggcggcgacc ctggtccgtg
    15001 gcccgtcagt ggctgggccg cttggtccgg ccggctcggt cacggccacg gccacggcca
    15061 ctcgcacggt cgactcggcc acggccacgg ccacggccac ggccactcgc acggtcgact
    15121 cgggcacgtc gggagacacg agcgctcaag ccgctgctga ggaccgaatc atccgggcgg
    15181 cgctggcggc ggatccggcg ttcccggcgg gctgggaccc ccagtcccag gcggtgttcg
    15241 gctgcggctt ctatcgcgtg ggcgcgtccg ccgacctcac ctatcgctat gtctgggcga
    15301 actgttcgat gttccagggc gacacggtcg tctcagggat gtcggtgcca ctctggctgc
    15361 acgtccgagg cagtggggag tcgctcgagg tcctgggcca cgagatcccg ggtgacggca
    15421 cggcatacac ggcggatgtc cgtcggctct tcccagccga tctggtcgac acgttcctcg
    15481 gcgccccgac gaccgagatt gtgagggtgg ctggcgcgct ggcgctgcgc gacgcccgcg
    15541 agcagcgcgc cagcgccgcc ggaaccaccc cgacagccgc cccgtagcgc gaaagtcgtc
    15601 gacccctctg gcgggctgcc tgaaggtcgt cgatgtttgg cgaggtccac gatatttcgt
    15661 cgaccttgcg aaaggtcgtc gaccattcgg tgcttcaggc ggtcgcgcgg agcggactga
    15721 gcaagcgggc gacgcgctca gggttgctca gatcgctcca cagcagtcgg accactcgat
    15781 atcccaaccg gcggagggcg tcttcgcggc gcttctcggc gatgaggtgc tcgcgaccct
    15841 cgggtcctga atacttcacc gccccgtcga actcgacgac gactcgcgcc tgctcaaaga
    15901 ggaagtcgac gcgggcgacg aacctgccct cctcgtcgaa gatctcgacc tgctggcggg
    15961 gttgagccag gccaaggtca gcgaacacca accgacacca ggactccccg gcggactcgc
    16021 tcgctgcact cgccaagtcg acgatgcgcg ccgcttgtcg tggacgcgga acactcgcga
    16081 cggccgccgc cagggcgtcc cgagtgaaga gccgcgatcg aagcccggcg tctgcagcca
    16141 tcaggcccgc tcggggaccc accagcgcgg ccgtttgcgc gactgcaacc gagggggatg
    16201 cgacgtgcag gcccgatcgc ttggtcgttg ccgccgagtc gatcgcgggg tggacccaca
    16261 agcccgtcgc ccgacgcgac cggtcgaagt cgatgccgct gacatgcacg atgtcgaggt
    16321 ctcggcgcaa caccggaagc gaccacgcga gcaacgccgt ctggtgactg agcgcgtgtc
    16381 gtggcatccc ggcgagcacg gcgcgggcgg tcagcagatg ccgtggcccg gcatccaggg
    16441 tcttggacgt ggccccgtcg ccgtagcgcc cgtgccacaa ccggaccagc aggccctggc
    16501 gcaccagcga gctcacgtcg ttgagcgaat accccagtcc gcgcagcgcg tggtaactga
    16561 cgacgccatc cccggccgcc aacatgcgca gcacggtctc gtcgagggcg gcatacctcg
    16621 ccggtcgtcg gtctcgaagg gcggcgctcc ccgccgcttg cggctctcgc atagcggcat
    16681 accttgccgc cttcgggggg ctcgcgggca ggggtatgcc gcgcctgtgg acagcgggac
    16741 agccgaccac gggggtgtgg acaaagcacc gcgctggcac gcccagcgag cccaggggac
    16801 gcggcagccc ccgaaaggtc gtcgaccttc gcattggtcg acgaaagatc gtcgagcaag
    16861 cgaaaggtcg acgatgtctt ggcgggcggg ggagcgtggc gggcggtgag cgaggtgggg
    16921 agcgaggccg gacggtccgc ggagggtcag gtcagcgtgg gggcatcgga gtgccgatgg
    16981 gccactcgac gagggcaccg gcatccgtcc gccagatcgc ggtcgtcaac cccgagttgg
    17041 ccaacaactt ctgacagccc gggcacggcg gatgggtgat cgcgatccag ccgccacgtc
    17101 ggcgcacggg gtcggcgaag agcaacgcat tggactcggc atggatcgag atgcagaacc
    17161 ccggcgacga cggatcctca taaggcgcat ccggcggcaa ctcgtcgtgg ccgagcgacc
    17221 cacgcgggca gccgccctcc aggcaaccga cccgcccggc cggcgccccg ttgtaacccg
    17281 tcgcgaggat ccgattggcc tcgtcgaaga ccacggctcc cacccgccgc cgggtgcagt
    17341 cggcccgctg ggcgacggca tacgcgatgc cgactcccca gtcgtgccac ccggggcggg
    17401 tggagaaggc tgtggtcacc cgccgagggc tgcgctgacg acgtcgcgtg cctcagcctg
    17461 cacctgtgcg aggtgctcgg cgccgcggaa ggactcggcg tagatcttgt agacgtcctc
    17521 ggtgcccgac ggccgggccg cgaaccacgc cgactcggtg acgaccttga gcccgccgat
    17581 cttggcgccg ttgcccggag cctcggtgag cttggccgtg atcggctctc ccgcaaggga
    17641 atccgccgcc acatcggcag gagacaaggc tcccagcttc gccttctgct cgcggttggc
    17701 cggggcgtcg atccgcgcgt atgccggtgc gccatgtttc gcggtcagct cgcggtagtg
    17761 ctcgctcggg ctggacccgg tgcgggcgag gatctccgag gcgagcaggg ccagcaggat
    17821 cccgtccttg tcggtcgtcc acaccgagcc gtcgaagcgg gcgaacgacg cccccgccga
    17881 ctcctcgcct ccgaacggcc ccgacccgtc gagcaacccg ggaacgaacc acttgaaacc
    17941 caccggcacc tcgaccagcc gccgcccgag gtcggaggcc acccggtcaa tcatcgacga
    18001 ggacaccagc gtcttgccga tgaacccgtc cggccgccaa cccggccgcc ctccgccata
    18061 caggtattgg atcgccacgg ccagatagtg gttggggttc atcagcccac catcaggcgt
    18121 gacgatgccg tgccggtcgg agtcggcgtc gttgccggtc gagatcgcga aggcgtcctt
    18181 gttggcgatg agggaagcca tcgcataggg cgaggagcag tccatccgga tcttgccgtc
    18241 ccagtcgagc gtcatgaacc gccaagtcgg gtcgacgagc gggttgatga ccgtgaggtc
    18301 gatccccaaa cgctcggcca tcgcgcccca gaactccacc gacgcaccgc cgagcgggtc
    18361 ggcaccgatc cggatccctg cggccttgat cgcgtcgatg tccacgacgt tcggcag
//
LOCUS       DAOMZY010000018        18372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_2675_length_18372_cov_6.293880, whole genome shotgun sequence.
ACCESSION   DAOMZY010000018 DAOMZY010000000
VERSION     DAOMZY010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 18372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18372
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2675_length_18372_cov_6.293880"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PLK64_02130"
     CDS             <1..261
                     /locus_tag="PLK64_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HPV78568.1"
                     /translation="SAMVEMTLPPTSPYAGARVGDIPWPEDTVLVGIIREGRPIAPSR
                     DDALEPQDELLFIAARTMEDNLENLLSPGHLPRGKDEDDDDE"
     gene            complement(307..1137)
                     /locus_tag="PLK64_02135"
     CDS             complement(307..1137)
                     /locus_tag="PLK64_02135"
                     /inference="COORDINATES: protein motif:HMM:NF022805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3159 domain-containing protein"
                     /protein_id="HPV78569.1"
                     /translation="MADQTDAGRPAAQESFVTVEEVLRARLGTVFGGWRGAVESAVPT
                     VLFVVVWNVTSDIPLAALSALAAAVVIAGIRLAQGQTLRYVGWAVIAVVISGVVATRT
                     GRAQDAFLPGMLQTGFSLLVFTISNLVRWPVIGFLIAAGDPELMQTSARVRFAGSREG
                     RAAMARMADEQRAAAEQAAEADAQAMSHAFTSWRRHDGIVKVASRLGWVLVGLAVLRL
                     SIQIPLYLQGAVEALGVAKLVLGWPAYLLAVGAGALLLLRGHTPLDAPSSAAADATDD
                     "
     gene            complement(1130..1474)
                     /locus_tag="PLK64_02140"
     CDS             complement(1130..1474)
                     /locus_tag="PLK64_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HPV78570.1"
                     /translation="MGRLTKSSAELQADELHDASLRLGATPISALVPRQRVTVSGEVR
                     SVALRPQVQVPALVAEVFDGTGTLILVWLGRRAVAGIVPGVMLRIHGRVTDLRAGPTI
                     YNPAYEILPQHG"
     gene            complement(1531..2307)
                     /locus_tag="PLK64_02145"
     CDS             complement(1531..2307)
                     /locus_tag="PLK64_02145"
                     /inference="COORDINATES: protein motif:HMM:NF023917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3710 domain-containing protein"
                     /protein_id="HPV78571.1"
                     /translation="MGLFGRKRAKDETERPVAETGADGIPASLPSDAEATAATVAESD
                     DWEPETPFSRLRGPFDRAEVTRDDTRVELGSLWVAITDGVQVALQVDESTQTPVGIQL
                     SLGTSGAYLQAYAAPRTEGIWPEIRTEVAQSVVAAGGRAELVSGPLGKELRLHQRGGV
                     PIRLLGVDGPRWFLRALVTGPAAVDEQAGDALIEVIRKTVVDRGTQAMAPREQLPLRL
                     PQADGPEAARPDASPGGEPTPAGFDDLQPFERGPEITEVH"
     gene            complement(2361..2810)
                     /gene="dut"
                     /locus_tag="PLK64_02150"
     CDS             complement(2361..2810)
                     /gene="dut"
                     /locus_tag="PLK64_02150"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPV78572.1"
                     /translation="MPGVPVQVHRLDPDLPLPAYAHPGDAGIDLFARVDVLLAPGQRA
                     LVPTGIAIALPEGYAAFGHPRSGLAIRHGITILNAPGTIDSGYRGEIFVNLVNLDASE
                     SFQVSRGDRIAQLVVQRVEAVQWVEVDSLPDSPRGDTGHGASGGFGA"
     gene            complement(2810..3109)
                     /locus_tag="PLK64_02155"
     CDS             complement(2810..3109)
                     /locus_tag="PLK64_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 domain-containing protein"
                     /protein_id="HPV78573.1"
                     /translation="MATDYDAPRKTDDDLSEDSLEELKARRVDKASSTVDVDETEQAE
                     GFELPGADLSGEELSYRVIPRQADEFTCSRCFLVHHRSQLAKNVGGQMVCRECVA"
     gene            complement(3349..4248)
                     /locus_tag="PLK64_02160"
     CDS             complement(3349..4248)
                     /locus_tag="PLK64_02160"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPV78574.1"
                     /translation="MTPIPDTTGHLPVPSIDDLSVLEVLALTLAHETGQLVHEGRPAD
                     LANTTGTKTSDTDPVTVMDTRAEEHLRRRLRAARPDDGLQGEEGSSIPSRSGLTWVVD
                     PIDGTTNYLYDLPLYAVSVAVVVGDPTTPGAWRPVAGAVRAPALDITWSARAGGGAWR
                     RGPARPWDVAGPGGEPPAVPARVGRQSHLGPALVGTGFGYQPERRAQQAEVLTRVLPA
                     VRDLRRMGSAAIDLCLVGDGRLDGFFEVGLNPWDLAAGWLVVTEAGGSVSGVGGGAPG
                     VDLVVAANPALHPRLLALLESPA"
     gene            4313..5506
                     /gene="sepH"
                     /locus_tag="PLK64_02165"
     CDS             4313..5506
                     /gene="sepH"
                     /locus_tag="PLK64_02165"
                     /inference="COORDINATES: protein motif:HMM:NF040712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SepH"
                     /protein_id="HPV78575.1"
                     /translation="MTTRPQPTHGVSMQDLRLIGVDDDGGHLLLASVDGARFRVPLDE
                     SLRAAARRDRPRLGQLQIEIEGGMRPRDVQAMVRAGMSADEVAERSGWTVEKVHKYEG
                     PILAEREYVAGLGRAVRLRQRGGSGPSTTLGQRVADRLTARGVEADSIEWDAWRREGP
                     EWTVAVNFSAGGRVRQGTWAFDVASRSLHAKDDEARWLSEDEPAATPVPREAPVYDVE
                     ADGGLDAAGRRSRADDPVDLVTAMRERSAARSRRGSRRRGLEPVAALPLGDAVQSPPD
                     DHVDTVETIPSDVAAAEERRPVAVTAAVTREAEPELAIAPGPDSITESSTAASTDSNT
                     EPDSEPKHEPAFEPRQDPKPEVEVEEVSPEPAVARHTPRRSGRPSVPSWDDIVFGTKP
                     KDPNA"
     gene            complement(5529..6191)
                     /locus_tag="PLK64_02170"
     CDS             complement(5529..6191)
                     /locus_tag="PLK64_02170"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HPV78576.1"
                     /translation="MAELVFFSGTMDSGKSTLALQMEHNHRARGKGGLIFTKHDRAGE
                     AVVSSRLGLSRRAIEVTDTLDFWDTVVGFVTEGHRVDFVVCDEAQFYTPRQIEQLARI
                     VDEMGVDVFAFGIMADFRTELFPGSRRLVELADRVQVLQVEALCWCGVRATHNARVID
                     GEMVIEGDQVVVGDTAKGSGVVEYEVLCRRHYMRRMTSHAARAAALSPDVLPFDVARQ
                     DG"
     gene            complement(6203..7366)
                     /locus_tag="PLK64_02175"
     CDS             complement(6203..7366)
                     /locus_tag="PLK64_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPV78577.1"
                     /translation="MGSAPPALPVGAGTLSAVLPSVLASLGSPRYAARPLIPLTPARR
                     AVVVLVDGLGDTLVRRRAGHAPFLRSGLAAAYRLTCGFPSTTATSMGSFGTGLPPGAH
                     GLVGYEVLVPGATPETDRILNELSWDDGPDPFTWQPEPTVFEAAEAEGVAVTRVGPGF
                     FDGSGLTNAALRGGRFRAAYQLATRVDEALAAVRATPRALVYLYWGDVDKLGHVHGPS
                     SPEWGAEVERVDAELRRLVASVPSDTAVYVTADHGMVEVPFADRIDLAVEPRLLDGVR
                     HVGGEPRSVQLYCAPGAAQDVSETWRERLGENATVISRSEAIEAGLFGPAGSVADRVL
                     PRIGDVLVNCLGSMAVVDSVRMRPELIALLGLHGSVSEDEIAIPLFQWPARTA"
     gene            complement(7368..7958)
                     /locus_tag="PLK64_02180"
     CDS             complement(7368..7958)
                     /locus_tag="PLK64_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5998 family protein"
                     /protein_id="HPV78578.1"
                     /translation="MRKVPREVPGFSLPVELRSDIQHAGYYPALVADVVGATLAGDVV
                     QAHLVHQETTFDQDSVRRHITVLVLTATKLVVVHADDHDDHHERAVATATSESIPLAA
                     VRGVMLAHVVANPDAYVPGSLGREITLTLGWGAVSRLDVMPASCGDPQCEADHGFEGT
                     LAADDISLRISADADGDAALARALHFASALSATIGR"
     gene            complement(7989..10718)
                     /locus_tag="PLK64_02185"
     CDS             complement(7989..10718)
                     /locus_tag="PLK64_02185"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV78579.1"
                     /translation="MSSKHGQHRHPLPDEPTSPEPVGGGDEHPPGYPFEWEADVVLSD
                     GSVAHVRPIRPSDDERIRVFHAGQSEESIYLRFFAPLRELSDRDVQRFTVVDYDERVA
                     LVVMHRDLIIGIGRYDKIDDTRAEVAFNVSDAFQGKGIGSILLEHLALVGLDGGVTRF
                     VAEVLPQNRRMINVFKEAGYNVKHRLEDGVISLSFDISPTATSTAVRYAREHRSEAIS
                     VGGILTPRSVAVVGASRREFSIGHTFLKNLVEGGFTGQVYAVNPNAKRVMGLKSYRTL
                     ADVPGLVDLVVVAVAAPDVINLVDECAAKGARAIVVPSAHFAEDGEQGWKLQTKLRKK
                     ARRAGLRVIGPNSFGIINNHPDVRLNASLAPQLPPRGSFGLFAQSGALGIAVLASAAR
                     RGLGVSVFASAGNRVDLSGNDLMQYWIEDASTDAVGLYVESIGNPRKFVRIARSLAAA
                     KPVIVVKSAGSRLGSAPGHLTRKMSVGTEAFDALLRQSGVIRTENIHQLFDVAQLVVH
                     QELPAGDRVAVVTNSDALGSLTTQAAQSWDLKVTHGPVSVAPEAGAEEFALALRAAFD
                     DPGVDSVIASFIPPIYTDDELVAAAVRDEAGRSTKTCTATFLGMRGVTEQLTLNTPGE
                     RPRVVPAYPMPEDAVRAVAAATRYAQWRARDKGTLVAPSGIEKGVVEELIDNVLTDSP
                     DGRALTEDECIQVLAAYGIGLWPADLVHNADEAVEAAERLGYPVILKVIAPALRHQVG
                     VTGVRPDLSSPEAVRDAHASLTNRLGAFVADRFAVQRMSPPGVSCVVGSTEDPLFGPV
                     VSFSIAGPPTDLLGDIGYRIPPLTNVDVDDLIFSVKAAPLLTGHGGKEPIYLDDLRDL
                     IARVSTLADNHSTLASVSLQPVNCWGAGVDVLGAEIFVAPAMTRKDASRRAMT"
     gene            10780..11397
                     /locus_tag="PLK64_02190"
     CDS             10780..11397
                     /locus_tag="PLK64_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPV78580.1"
                     /translation="MELSRAIRGRRMIRRYDPDRPVPDEAVLACLGAAIRAPSAGFSQ
                     GWDFLVLRSPEERATFWSATTGDQPPDTWLAGMATAPALILCLSDPERYLERYAQPDK
                     GWTDRDPARWPVPYWDVDTGMAAMLALLTAVDAGLGACFFGVPPTRHTEVRSALGIPD
                     DRRFVGVISLGYAVPHPASPSLARGRRGLDEVAHWGRFGVHDPLP"
     gene            11644..14124
                     /locus_tag="PLK64_02195"
     CDS             11644..14124
                     /locus_tag="PLK64_02195"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016664672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 4 subunit A"
                     /protein_id="HPV78581.1"
                     /translation="MARRTTTQPPDGEDVVERIVDIDVAEEMKAAFLEYSYSVIYARA
                     LPDARDGLKPVQRRILFGMSELGLRPERPHVKSSRIVGEVMGKYHPHGDSAIYDTLVR
                     LAQPFSMRLPLVDGHGNFGSLDDGPAASRYTEARMAPGAMLMVAALDEDTVDFVPNYD
                     DTQTQPEVLPAAFPNLLVNGASGIAVGMATSMPPHNLGEVIGAARHLIEHPDATLDDL
                     MRFVPGPDLPGGGRIVGLDGIREAYAGGRGTFRTRATARIENITPRRRGIVVTELPYL
                     VGPEKVIEKIRDQVQSKRLLGISDVKDLTDRSNGLQLVIEVKSGFVPEAVLEELYRLT
                     PMEDSFGINNVALVEGQPRTLGLVELLSVFVDFRIGVVRRRTAHRLAKREERLHLVDG
                     LVVAILNIDEVIQLIRAADDTATARARLMGVFDLSEAQATYILELQLRRLTKFSRLEL
                     EKEQEQLRSEIEALRAVLSDERLLRRTVSDELAEVAQAHATPRRTVLLESAGAVAGVT
                     AAAPARGQSAVLEIADDPCWVLLSSTGLLARTTSADDLPTGGGRARHDVIIGAVRATA
                     RGEVGLVTSLGRMLRLSVLELPTLPPTADSPHLSGGAPLAAYLELGRDEQPVTLASLA
                     PDSPVLALGTASGVVKRVVTDHPARPSWELIALRDGDQVVGAAPTSSDEIDLVFLTSD
                     AQLLRFSAGSVRPQGRAAAGMAGIRLDPAARALFFGVVDPTRDGLVVTASGTSGALPG
                     TQAGAIKVTPYAEYPAKGRGTGGVRAHRFLKGEDTLVLAWAGSVPARAAAANGGPVDL
                     PAATGRRDGSGATWSHVVAAIAGPASGA"
     gene            14485..15813
                     /locus_tag="PLK64_02200"
     CDS             14485..15813
                     /locus_tag="PLK64_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPV78582.1"
                     /translation="MRRSTFAKAATGAAAIALLTSGCLGSSGTTTTAATGTAAGAGKA
                     TVEVMYGFGNEQETAFQKDLKAFADKSGFTIKFTKAGSWDTEIKTRVAGGNPPDVGLF
                     PQPGVMCDLAKQGKVLAYDDATVTSAKATLVNGFVGAGTCTDGKVYGLPSAVSVKSLL
                     WYDAPVFAAAGYKAPTTLAELDALTEKIKADGKTPWCIAAESGGATGWPITDWIEDLV
                     LRFAGPENYDKWVAGTLKFNSPEIKPAWDYFQKIAFTEGNVRGGTKAIAATNFQTGGN
                     VLFDTPPACHLFKQATFIAGKGGFPDTVLAKADTEIGVVAFPAKEAGNNPVLTGGDLA
                     TAFNNDANTLKLRNFIASKDNGVEVGKAGYFSPHKSFDVTLYPSKMLQKIASDVLYKS
                     SAARFDGSDLMPAKVGAGTFWTEPIKWISGTQDLDVTLNNIDASWPKAAS"
     gene            15910..16893
                     /locus_tag="PLK64_02205"
     CDS             15910..16893
                     /locus_tag="PLK64_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPV78583.1"
                     /translation="MTIKILNALLAIIGGIGGAMILFWLLNKLAESLKGRWEDRVKPW
                     VFAGPAILAIGVYLIYPAIVTLQYSFANEDATKYVGFNNYKDLLTDATFLSVLFNNLL
                     WIIIVPALTVALGLGVAVLADRLRPKGEKLSKTVIFLPMAISMVGAATIWRSIYEYQP
                     KGSPQTGLLNAIMGVFGKDPVYWYQIETWHLNSLLLMVILIWTQVGYSMVLLSSAIKG
                     VPEDTIEAGRIDGAGERRIFFSIVVPQIWPTVVTVFITVLIGVMKVFDVVYVTTNGAF
                     NTDVVGRRFFYEMFTNQNSGYAAAIVVLLMIAITPVLVYQVRQFRAEEANR"
     gene            16890..17807
                     /locus_tag="PLK64_02210"
     CDS             16890..17807
                     /locus_tag="PLK64_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPV78584.1"
                     /translation="MTTAVPPSTDAQVIRQSKAGSGPGSATWPVRIFVTFLCLIWLIP
                     VLGLLISSFRTRDAQFNSGWWTAIANPLEFTQWTVNNYAQVLTTNDSGINMGQAFINS
                     FIVTVPATVIPILIAAFAAYAFTFMEWKGRDLVFVIIVGLLVVPNQVALVPLLKLYSS
                     LGLNGTFLAVWLAHIGFGMPLAVYILRNYMSSLPKAVIESAKIDGASHFTTFWRLIIP
                     MSTPALASFAIFQFLWVWNDLLIALLFLGRGENTIATVALQGLQGQTGQGRELIPSAG
                     FITIIVPLMVFLSLQRYFIRGMTSGAVKG"
     gene            17914..>18372
                     /locus_tag="PLK64_02215"
     CDS             17914..>18372
                     /locus_tag="PLK64_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 66 protein"
                     /protein_id="HPV78585.1"
                     /translation="MLPTRSLFRATDRVAVEIRDLRQSAELTVHRDGAVLSSHPVSSA
                     GVIPLGELEPGSYGISLRAGDQQIARSAVQIAGSGGDRGILRYAFVADYRPDRSVADL
                     AAVADNLRRLHITGVQFYDWAYRHADLLGGGERYADALGSPVDLGTVRALI"
BASE COUNT         2979 a         6422 c         6164 g         2807 t
ORIGIN      
        1 tcggccatgg tcgagatgac cttgccgccg accagcccct acgccggcgc gcgggtgggc
       61 gacatcccct ggcccgagga caccgttctc gtgggcatca tccgggaggg tcggcccatc
      121 gcgcccagcc gcgacgatgc gctcgagccg caagatgaac tgctgttcat cgcggcgcgc
      181 accatggaag acaacctcga gaacctgctc tcccccggcc acctgccgcg cggcaaggat
      241 gaggacgacg acgacgagtg acgacacccg ccgcacggtc gagggaagga cctcgccgca
      301 cgcggctcag tcgtcggtgg cgtccgccgc tgcgctgctg ggggcgtcca gcggagtgtg
      361 tccacgcagc agcaggagcg ctcccgcccc taccgccagc aggtatgccg gccagccgag
      421 cacgagtttg gccaccccga gcgcttccac cgcaccctgc agatagagcg ggatctggat
      481 cgagaggcgc agcacggcca gccccacgag cacccagccg agccggctgg ccaccttgac
      541 gatcccgtcg tgacggcgcc aggacgtgaa cgcgtggctc atggcctgcg cgtcggcctc
      601 ggcggcctgc tccgccgcgg cccgctgctc atcggccatc cgggccatcg cggcccgtcc
      661 ctcgcgggaa ccggcgaacc ggacccgggc actggtctgc atgagctccg ggtcgcccgc
      721 cgcgatgagg aagccgatga ccggccagcg cacgaggttg gagatggtga agaccagcag
      781 ggagaacccg gtctggagca tccccggcaa gaaggcatcc tgggcccgcc cggtgcgcgt
      841 ggccaccact cccgaaatga cgacagcgat caccgcccac ccgacatagc gcaacgtctg
      901 accctgggcc aggcggatcc cggcgatgac gacggccgct gcgagggccg acagggcagc
      961 gagggggatg tcgctggtga cgttccatac gacgacgaag aggacggtcg gcacggccga
     1021 ctcgacggcg ccgcgccagc ctccgaacac ggtcccgagg cgggcccgca acacctcttc
     1081 gacggtgacg aacgactcct gggcggccgg acgacccgca tcggtctgat cagccatgct
     1141 gcggcaggat ctcgtaggcg gggttgtaga tcgtcggccc ggcgcgcagg tcagtgaccc
     1201 ttccgtggat ccgcagcatg accccgggga cgatccccgc cactgcccga cgaccgagcc
     1261 agacaaggat gagcgtgccc gttccgtcga agacctctgc gaccagggcc gggacctgca
     1321 cctgaggccg cagcgccact gagcgaacct cgccgctcac ggtcacccgc tgccgaggga
     1381 ccaacgctga gatgggggtc gctcccaggc gaaggctggc gtcgtggagc tcgtcagcct
     1441 gcaactcggc cgacgacttg gtcagccgac ccatgagccg gctccatgtg ctcgactcgg
     1501 aacgacccgg gctgccctcg tcagacatgg ctagtggacc tcggtgatct cagggccccg
     1561 ttcgaagggc tgcaggtcat cgaaccccgc cggggtcggc tctcccccag gcgatgcgtc
     1621 gggacgcgcg gcttcggggc cgtcggcctg gggcagccga aggggcagtt gctcgcgcgg
     1681 cgccatcgcc tgggtgcccc ggtcgaccac cgtcttgcgg atgacctcga tgagcgcgtc
     1741 gccagcctgc tcgtcgacgg cggcggggcc ggtgacgagc gcccgaagga accaacgggg
     1801 gccgtccacc ccgagcagcc ggatcgggac gccgccgcgc tggtgcagcc gcaactcctt
     1861 gccgagcggg ccgctcacga gctccgcgcg accgcctgcc gcgacaaccg attgagccac
     1921 ctcggtgcgg atctcaggcc agatgccctc ggtgcggggc gcggcatacg cctgcaggta
     1981 ggcgcctgac gtgcccaagg agagttggat gccgaccggg gtctgcgtcg actcgtccac
     2041 ctgcaacgcg acctggactc cgtccgtgat cgccacccac aggctgccca actcgacccg
     2101 tgtgtcgtcg cgggtcacct cggcacgatc gaaggggccg cgcagccggc tgaacggcgt
     2161 ctccggctcc cagtcatcgg actcggcgac cgtcgcggca gtcgcctcag cgtcactcgg
     2221 cagggacgcc ggtatgccgt ccgctccggt ctcggccacc ggccgttccg tctcgtcctt
     2281 ggcgcgcttg cgcccgaaaa gtcccacgtt gcgtcatcca gtcctgttgg gcagaggtcg
     2341 ttcggcctgc ggcggcgggg tcaggccccg aagccgccac tggctccgtg tccagtgtcc
     2401 ccccgggggc tgtccgggag cgaatcgacc tccacccact gcaccgcttc gacgcgttgg
     2461 acgaccaact gggcgattcg atcgccgcgg ctgacctgaa agctctcgga ggcatcaagg
     2521 ttgaccaggt tgacgaagat ctcaccgcga tagcccgagt cgatggttcc gggcgcgttg
     2581 aggatggtga tgccgtgacg gatcgccagc cccgagcggg ggtggccgaa cgcggcatac
     2641 ccctcgggga gggcgatcgc gatgccggtg ggcacgagcg ctcgctgccc gggggcaagg
     2701 agcacgtcga cacgcgcgaa caggtcgatg ccagcgtcgc ccggatgggc gtaggccggg
     2761 aggggcaggt ccgggtcgag tcggtgcacc tggaccggca cgccgggcat caggcgacgc
     2821 actcccggca gaccatctgc ccaccgacgt tcttggccaa ctgggaccgg tggtgcacga
     2881 ggaagcagcg ggaacacgtg aattcgtcgg cctggcgcgg aatcacccgg taggacaact
     2941 cctcaccgga caggtcggcg ccgggaagtt cgaagccttc ggcctgctcg gtctcgtcaa
     3001 cgtcaacggt cgaggacgcc ttgtccacac gtcgggcttt gagctcctca agggagtctt
     3061 ctgacagatc gtcgtcggtc ttgcgtggtg cgtcgtagtc ggtcgccatg cccgttcggt
     3121 ctcctccatc actgtcggcc tgcggtggct cgtcgcggct aacgctcaga tcgtcgaatt
     3181 tgtgcccggc tgcaccgggg ccaacgacac gtcactgcct gcggggcgtg attgtgccct
     3241 attcagaggc cgagcgcaca tgcgtttcgc atatcgattg ggtcacacag gacgggtcgc
     3301 ccctgcaagc ggtcttacct ccgatcgctc cgagggtcga cctgcgccct aagccggtga
     3361 ctccagcagc gccagcagcc gagggtgcag ggccgggttg gccgcgacga ccaggtccac
     3421 accaggcgcg ccaccgccga ccccggagac agatcctccg gcctcggtga cgaccaacca
     3481 cccggctgcg aggtcccacg ggttgagacc gacctcgaag aagccgtcca gtcggccatc
     3541 gccgacgagg cacaggtcaa tggcggcact gcccatccgg cgcaggtcgc gcacggccgg
     3601 caggacgcgg gtgagcacct cggcctgttg ggctcggcgt tcgggctggt agccgaagcc
     3661 ggtgccgacg agcgcggggc cgaggtggct ctggcggccc acccgcgccg ggaccgccgg
     3721 cggctcgccc ccgggcccgg cgacatccca cggccgcgca ggtccgcgcc gccacgctcc
     3781 cccgccggcc cgggccgacc aggtgatgtc cagggcaggg gctcggactg ctccagcgac
     3841 cggacgccag gcgcccggcg tggtggggtc gcccacgacg acggcgacgg acacggcata
     3901 caggggaagg tcgtagaggt agttggtggt gccgtcgatg ggatcgacca cccaggtgag
     3961 cccgctgcgg gacgggatcg agctgccctc ctccccctgc agaccgtcgt cgggacgtgc
     4021 cgccctcaac cggcgccgga ggtgctcctc ggcacgggtg tccatgacgg tcaccggatc
     4081 ggtgtccgac gtcttggtcc ctgtcgtgtt ggccaggtcg gccggtcgcc cctcgtggac
     4141 cagttgaccg gtttcgtgag ccagggtgag cgccaatacc tccaaaacgg acaggtcgtc
     4201 aatggatggg acgggcaggt gcccggtcgt gtctgggatc ggcgtcatgg tgaggtccac
     4261 ggtaacggtc cggcgtggcc gcttcgcaga cctcggttcg aatgagagag tcgtgacgac
     4321 acgtccccag ccgacccatg gagtgagcat gcaggacctg cggttgatcg gcgttgacga
     4381 cgacggcggg cacctcttgc tcgccagcgt cgacggcgcc cgattccggg tcccgctcga
     4441 cgagtcgttg cgggctgcgg cccgccgtga ccgtcctcgg ctgggacaac tgcagatcga
     4501 gatcgagggc ggcatgcgac cacgcgacgt gcaggcgatg gtccgggcgg ggatgtccgc
     4561 cgatgaggtc gccgagcgct ccgggtggac ggtcgagaag gtccacaagt acgaaggccc
     4621 gatcctcgcc gagcgggagt atgtcgccgg cctcggccgg gcggtgcggt tgcggcaacg
     4681 cggcggatcc ggcccctcga ccacgctggg ccaacgcgtc gccgatcgac tcaccgcccg
     4741 cggcgtcgag gccgactcga tcgagtggga cgcctggcgc cgcgaaggac ccgagtggac
     4801 ggtcgcggtc aacttcagcg ccggtgggcg ggtgcgccag ggcacctggg ccttcgatgt
     4861 cgccagccgt tcgctgcatg ccaaggacga tgaggcccgc tggctcagcg aggacgaacc
     4921 tgcggcaacc ccggtcccgc gtgaagcacc ggtctacgac gtcgaagccg acggcggact
     4981 ggatgcggcc ggacgccgct ctcgggccga cgacccggtc gacctcgtca cggcgatgcg
     5041 tgagcgttcg gcggcccggt ctcgccgcgg gtcccgccgt cgcgggttgg agccggtggc
     5101 tgccctgccc ttgggcgatg ccgtgcagtc gccccccgac gaccacgtcg acaccgttga
     5161 aaccatcccg agcgacgtcg ccgccgccga agagcgccgg cccgtcgcgg tgaccgccgc
     5221 cgtcactcgc gaggccgagc cggagcttgc catcgcgccc ggtcccgact caattaccga
     5281 atccagcacc gccgccagta ccgactccaa caccgagccg gactccgagc ccaagcacga
     5341 accggccttc gaacctcgcc aggaccccaa gcctgaggta gaggtcgagg aagtttcccc
     5401 ggaaccggcg gtggcacggc atacccctcg gcgctccggt cgaccctcgg tgccgagttg
     5461 ggacgacatc gtcttcggga ccaagccgaa ggaccccaac gcgtaacgcc gtcggcgcag
     5521 agcgcgggtc agccgtcctg gcgcgcgacg tcgaagggca gaacgtccgg tgagagcgcg
     5581 gcggcgcggg ccgcgtgcga ggtcatccga cgcatgtagt ggcgccggca gagcacttcg
     5641 tactccacga ccccactgcc cttggccgtg tcgccgacca ccacctggtc accttcgatg
     5701 accatctccc cgtcgatgac gcgggcattg tgggtcgcgc ggacgccgca ccagcacaac
     5761 gcctcgacct gcagcacctg gactcggtcg gcaagctcaa ccaagcggcg cgacccgggg
     5821 aagagctccg tccggaagtc cgccatgatg ccgaaggcga agacgtccac gcccatctcg
     5881 tcgacgatcc tcgccagttg ctcgatctgc cggggcgtgt agaactgcgc ctcgtcgcac
     5941 acgacgaagt ccacccggtg accctcggtc acgaagccga cgacggtgtc ccagaagtcc
     6001 agggtgtcgg tgacctcgat ggcccgccgg gacaacccca ggcgcgagga cacgaccgcc
     6061 tcacccgcac ggtcgtgctt ggtgaagatc aacccgccct taccccgcgc ccggtggttg
     6121 tgctccatct gcagggcgag ggtcgacttg ccggaatcca tcgtcccgga aaagaacacc
     6181 aactcggcca cgacgcccga ccctatgccg tgcgggcggg ccactggaac agcgggatcg
     6241 cgatttcgtc ctcgctgacc gagccgtgca gccccaacag ggcgatgagc tccggtcgca
     6301 tccgaaccga atcgacgacg gccatgctgc ccaggcagtt gaccaagaca tcgccgatgc
     6361 gtggcaacac ccgatcggcc acggaccccg ctgggccgaa gagcccagcc tcgatggcct
     6421 cggaccggct gatcaccgtg gcgttctccc cgaggcgctc ccgccatgtc tcagacacgt
     6481 cctgggcggc gcctggcgcg caatagagct gcaccgaacg gggttcgccc ccgacgtgcc
     6541 ggaccccgtc aagcagccgc ggctcgaccg cgagatcgat ccggtccgcg aacggaacct
     6601 cgaccatccc gtggtcggcg gtgacataga cggccgtatc ggaggggacg gaggccacca
     6661 gccggcgcaa ctcggcgtca acccgctcga cctccgcgcc ccactccggc gagctcgggc
     6721 catggacgtg gccgagcttg tcgacgtcgc cccagtagag gtaaaccaaa gcgcgcgggg
     6781 tcgctcggac cgcggcgagg gcttcgtcga cgcgggtcgc gagttggtat gccgcgcgga
     6841 accggccccc gcgtagcgcc gcgttggtca gccccgaacc gtcgaagaag ccgggaccga
     6901 cccgcgtgac ggccactcct tcggcctcag cggcctcgaa gaccgtcggc tcgggctgcc
     6961 aggtgaacgg gtcgggcccg tcgtcccaac tgagctcgtt gaggatgcgg tcggtctcgg
     7021 gcgtcgcgcc cgggacgagc acttcgtaac cgaccaggcc gtgcgccccc ggaggcagcc
     7081 cggtcccgaa actgcccatc gacgtcgccg tcgtcgatgg gaacccacac gtcaatcggt
     7141 atgccgcggc gagcccggac cgcaggaacg gcgcgtgacc ggcccggcgg cggacgagcg
     7201 tgtcacccaa gccgtcgacc aggacgacga cagcgcgacg ggcgggggtc agcgggatca
     7261 gggggcgggc ggcataccgg ggcgacccga gggaggccaa gaccgacgga aggaccgccg
     7321 acagggtccc ggccccgacc gggagggccg gcggcgcaga acccacctca gcggccgatc
     7381 gtcgccgaca gcgccgaggc gaagtggagc gcgcgggcca gggccgcgtc gccatcggcg
     7441 tcggcgctga tgcgcagcga gatgtcgtcg gccgcgagtg tcccctcgaa gccatggtcg
     7501 gcctcacact gcgggtcacc gcacgaagca ggcatgacgt ccaggcggct cacggccccc
     7561 cagcccagtg tcagggtgat ctcccgaccg agcgaacccg gcacgtaggc atcgggattg
     7621 gccacaacgt gggcgagcat gaccccgcgc accgcagcca acgggatgct ctcactcgtc
     7681 gccgtcgcga cggcgcgctc gtggtggtcg tcgtgatcgt cggcatggac gacgacgagt
     7741 ttggtcgccg tgaggacgag aacggtgatg tgccggcgga cggagtcctg gtcgaaggtc
     7801 gtctcctggt ggaccaagtg ggcctggacg acatcaccgg caagggtggc gccgaccacg
     7861 tcggccacga gggccgggta gtaaccggcg tgctggatgt ccgacctcag ctcgacgggc
     7921 aggctgaaac cgggcacctc acggggaacc ttacgcatga tggccattct ctcccatggc
     7981 cgcctcggtc aggtcatcgc gcggcgactg gcgtccttgc gggtcatggc cggggccacg
     8041 aagatctcgg ccccgaggac gtcgacccct gcgccccaac agttcaccgg ttgcaacgac
     8101 accgacgcca gcgtcgagtg gttatcggcg agcgtcgaca ctcgggcgat gagatcacgc
     8161 aggtcatcga ggtagatcgg ttccttgccg ccatggccag tgagcagcgg ggcagccttg
     8221 accgaaaaga tcaggtcgtc gacatcgacg ttggtcaacg gcgggatgcg gtagccgatg
     8281 tcgccgagca gatcagtggg cgggccggcg atcgagaacg acacgacggg gccgaagagc
     8341 gggtcctcgg tgctgccgac gacgcaggac actcccggag gtgacatccg ctggacggcg
     8401 aaccggtcgg ccacgaaggc gcccagccgg ttggtcagcg acgcatgggc atcgcgcact
     8461 gcctcgggtg aggagaggtc gggacggact ccggtcacgc ccacctggtg gcgcagcgcc
     8521 ggcgcaatca ccttgaggat gacgggatat ccgagccgct cggccgcctc gaccgcctcg
     8581 tcggcgttgt gaaccaagtc ggcgggccac aagccgatgc cgtatgccgc gagcacctgg
     8641 atgcactcat cctcggtcag tgccctcccg tcggggctgt cggtgaggac attgtcgatg
     8701 agctcctcga cgacaccctt ctcgatcccc gagggggcca cgagcgtgcc cttgtcgcga
     8761 gcccgccact gcgcgtagcg agttgccgcc gccaccgctc gcacggcgtc ttcaggcatg
     8821 gggtatgccg ggaccacccg agggcgctcg ccgggggtgt tcaaggtcag ttgctctgtg
     8881 acgccgcgca tgccgaggaa cgtggccgtg caggtcttgg tgctgcgtcc ggcctcatcc
     8941 cggaccgccg cggcgaccaa ctcgtcgtcg gtgtagatgg gggggatgaa cgaggcgatc
     9001 accgagtcga ccccggggtc atcgaaggcc gcccgcaacg cgagcgcgaa ctcctcggcg
     9061 cccgcctcgg gggcaacgga gaccggcccg tgggtgacct tgaggtccca actctgggca
     9121 gcctgcgtcg tcagcgaacc cagtgcgtcg gaattggtga ccacagcgac gcgatcaccg
     9181 gccggaagct cctggtgaac gacgagttgg gcgacgtcga acagttgatg gatgttctcg
     9241 gtgcggatga cgcccgactg gcgcaacagc gcgtcgaacg cctccgtgcc gacactcatc
     9301 ttgcgggtga ggtgacccgg tgccgacccg agtcgactgc ctgcggactt gacgacgatg
     9361 accggttttg cggccgcgag cgagcgggcg atgcggacga atttgcgcgg attgccgatg
     9421 gattcgacgt agagacccac agcatcggtg gacgcgtcct cgatccagta ctgcataagg
     9481 tcgttgcccg aaaggtccac ccggttgcct gcagaggcga acaccgagac gcccaacccc
     9541 cgacgggcgg ctgaggcgag aaccgcgatg ccgagggcac cgctctgggc gaagagcccg
     9601 aaggagccgc gcggcggtag ttgcggcgcg agcgacgcgt tgagccgcac gtcggggtgg
     9661 ttgttgatga tgccgaagga gttgggcccg atgacccgca gcccggcccg acgggccttc
     9721 ttgcgcaact tggtctggag tttccacccc tgttcgccgt cctcagcgaa gtgagccgac
     9781 ggcacgacga tcgctcgggc gcccttcgcc gcgcactcgt cgacgaggtt gatgacgtcg
     9841 ggcgcggcca cagcgacgac gacgaggtcg accaggccgg ggacgtcggc cagcgtgcgg
     9901 taggacttga gccccatcac gcgcttggcg ttggggttga cggcatacac ctgcccagtg
     9961 aaacccccct cgacgaggtt cttgaggaag gtgtgtccga tggagaactc gcgcctgctc
    10021 gcaccgacga cggcgaccga acgcggcgtg aggatgcccc cgacgctgat cgcctccgaa
    10081 cggtgctcgc gggcataacg cacggccgtc gacgtggccg tcgggctgat gtcgaacgac
    10141 agcgagatga ccccgtcctc gagccggtgc ttgacgttgt agccggcctc cttgaagacg
    10201 ttgatcatcc gacggttctg cgggagcact tcggcgacga agcgggtgac cccgccgtcg
    10261 aggccgacca gcgccaggtg ttccaatagg atcgagccaa ttcccttgcc ctggaaggca
    10321 tccgacacgt tgaaggcgac ctcggccctg gtgtcgtcga tcttgtcgta acgcccgatc
    10381 ccgatgatca ggtcccgatg catcacgacg agggcgaccc gttcgtcgta gtcgacgacg
    10441 gtgaaccgtt ggacgtcacg gtcggacaac tcgcgcaacg gtgcgaagaa gcgcaggtaa
    10501 atcgactcct cgctctgccc ggcatgaaag acccggatgc gctcgtcgtc ggacggcctg
    10561 atcggccgca catgcgccac cgagccgtca ctgaggacga catccgcctc ccactcgaac
    10621 ggatagccgg gtgggtgttc gtcgccgcca ccgacgggtt cgggcgaggt cggctcgtcc
    10681 ggcaacgggt gccggtgttg gccgtgcttg ctggacacgc ccacatgttc ccacgccaag
    10741 gggtcacggg ttccctgcgg ggtggcagca tgcgaggtca tggagctttc tcgcgcgatc
    10801 cgtggccgcc ggatgatccg tcgctacgac cctgaccggc ccgtgccgga cgaggccgtg
    10861 ctcgcctgcc tgggggccgc catccgagct cccagtgccg gtttcagcca gggctgggat
    10921 ttcctcgtgc tgcgctcccc cgaggaacgg gcgaccttct ggtcggcgac gacgggcgat
    10981 caaccgccgg acacatggct ggccgggatg gcgaccgcgc ccgccctcat cctgtgtctg
    11041 tccgaccccg agcgatacct cgagcgttac gcgcagcccg acaagggctg gaccgaccgc
    11101 gacccggcgc gctggccggt cccctattgg gacgtcgaca ccggcatggc ggcgatgctc
    11161 gccctgctca ccgcggtcga tgcagggctg ggagcctgct tcttcggggt gccgccgacg
    11221 cggcataccg aggtccgttc tgccctgggg atccccgatg accgccgctt cgtcggggtc
    11281 atcagcctcg ggtatgccgt gccccacccg gcgagcccca gcctggcccg cgggcgccgc
    11341 ggcctggacg aggtcgcgca ctggggccgg ttcggggtcc acgacccact cccctgactc
    11401 cggcgcgcct gaccccctcc cgccctgcac ggagatagcg ctcgcatggc cacttttccg
    11461 tccttccgcg gcctcaaacc ccccacacat gaccgtgcga gcgctatctc cgtgtcaggg
    11521 ggcgcgtggg ggccgtgaga ggaccgggcg cgggaaccct cgtgtcgcag gtatgccgcg
    11581 tgggcgtcgt gggagaggat ggggcgttga tccgccgacc cactcgcagg agcccagccc
    11641 ggcatggccc gtcgcaccac cacccagccc cccgatggcg aggacgtcgt cgagcggatc
    11701 gtcgacatcg acgtcgccga ggagatgaag gccgccttcc tcgagtactc ctacagcgtc
    11761 atctatgccc gagccctgcc cgatgcccgc gacggcctca agccggtgca acgacggatc
    11821 ctcttcggga tgagcgagtt ggggctgcgg cccgagcgcc cgcacgtgaa gtccagccgg
    11881 atcgtcggcg aggtgatggg taagtaccac ccgcacggtg acagcgcgat ctatgacacc
    11941 ctcgtgcggc tggcccagcc gttctcgatg cgcctgcccc tggtcgacgg gcacggcaac
    12001 ttcggctcgc tcgatgacgg cccggccgcc tcgcgataca ccgaggcccg gatggcgccc
    12061 ggggcgatgc tcatggtggc ggctctcgat gaggacaccg tcgacttcgt ccccaactac
    12121 gacgacaccc agacccagcc cgaagtgttg cccgcggcgt tccccaacct gctcgtcaac
    12181 ggcgccagcg ggatcgccgt cggcatggct acgagcatgc cgccgcacaa cctcggcgag
    12241 gtcatcggcg ccgcgcgcca cctcatcgag caccccgacg cgaccctcga tgacctcatg
    12301 cgtttcgtcc ccggccccga tctgcccggc ggcggacgga tcgtcgggtt ggacggcata
    12361 cgggaggcgt atgccggtgg gcgcgggacc ttccgcacgc gcgccaccgc gcggatcgag
    12421 aacatcaccc cccgccgccg cgggatcgtc gtcaccgaac tgccctatct cgtcgggccc
    12481 gagaaggtca tcgagaagat ccgcgaccag gtccagagca agcggctgct ggggatcagc
    12541 gacgtcaagg acctcaccga ccgcagcaac gggctgcagt tggtcatcga ggtgaagagc
    12601 ggcttcgtac ccgaggcggt gcttgaggag ctctatcgac tgacaccgat ggaggactca
    12661 ttcgggatca acaacgttgc gctcgtcgag ggccagccgc gcacgctcgg gctcgtcgag
    12721 ttgctgtcgg tcttcgtcga cttccgaatc ggcgtcgtcc ggcgacgcac cgcccaccga
    12781 ctggccaagc gcgaggagcg tctgcacctg gtcgatgggc tcgtcgtcgc catcctcaac
    12841 atcgacgagg tcatccagct catccgggcg gccgatgaca cggcgacggc acgcgcccgc
    12901 ctcatgggtg tattcgacct gtcggaggcg caagcgacct acatccttga gctccaactg
    12961 cgccggttga cgaagttctc ccggctggag ttggagaagg agcaggagca gttgcgctcg
    13021 gagatcgagg ccctgcgggc ggtcctctcc gatgagcgac tgctgcggcg cacggtctcc
    13081 gacgagctgg ccgaggtcgc ccaggcgcac gccaccccgc ggcgcacggt gctgctggag
    13141 tcggctggcg ccgtcgccgg ggtcaccgcc gcggcccctg cccggggcca gagcgcggtc
    13201 ctcgagatcg ccgacgaccc gtgttgggtg ctgctctcct caaccggcct gttggcccgc
    13261 acgacatcag ccgacgatct cccgaccgga ggggggcggg cccggcacga tgtcatcatc
    13321 ggcgcggtgc gggccaccgc ccgaggcgag gtcggtctcg tcacgagcct gggccggatg
    13381 ctgcgcctct cggttcttga gttgccgacg ctccccccga ccgccgactc cccccatctg
    13441 tccggcggtg ctccgctggc ggcatacctc gagctgggtc gggacgagca gccggtgacc
    13501 ctcgcatccc ttgcgccgga ctcccccgtg ctggccctcg ggacggcctc gggcgtcgtc
    13561 aagcgcgtgg tcaccgacca ccccgcccga ccgtcgtggg agctcatcgc cctgcgcgac
    13621 ggcgaccagg tggtgggtgc cgccccgacc tccagcgacg agatcgacct ggtgttcctc
    13681 accagtgacg cgcaactgct gcggttctcc gccggaagcg tgcggccgca aggccgagcc
    13741 gccgcgggca tggccggaat ccggctggat cccgcggcca gggcgctgtt cttcggagtg
    13801 gtcgacccca ctcgggacgg gttggtcgtc accgcgagcg gcacctcggg agccctcccc
    13861 ggcacccagg cgggggccat caaggtgact ccgtatgccg agtacccggc caaggggcgc
    13921 ggcacgggag gggtgcgagc gcaccgattc ctcaagggcg aggacacctt ggtgctggct
    13981 tgggccggct cggtgccggc gcgggcggcc gcagccaacg gagggccggt cgacctgccg
    14041 gcagcgacgg gtcggcgcga cgggtccggc gccacctgga gccatgtggt ggccgctatc
    14101 gccggcccgg ctagcggggc ctagtatctc ggatcgacct gagtctcggc gctcgacgca
    14161 cctctgcgat gacgtcggtg atgacgtcgc tgacggcgtt gctcggcccg tgcgaaggcg
    14221 cgggctcgac gcataacaag gcgataaaca ccggccatga ctcttgacgg gaaagcgttt
    14281 acacggcaga gtcggccatg ccctcggaca cgtggggccg accggtcggg agaccgtccg
    14341 ggcgtatcag tcggttcatc tatggggtgc tcttcggaac cctccgtagc ctccacagac
    14401 gcgcgggagc aagccaccag cagcggctac gggtagccga agctgtgtca ctccatcccg
    14461 taagtagaca agggagtcaa acgtatgcgc aggagtacct tcgccaaggc agccacagga
    14521 gcagccgcga tcgcgctgct cacctcgggc tgtttgggct cgagtggcac gacgacgact
    14581 gctgccacgg gcacggccgc gggagccggc aaggccaccg tcgaggtgat gtacggcttc
    14641 ggcaacgagc aggagaccgc cttccagaag gacctcaagg ccttcgccga caagagcggc
    14701 ttcacgatca agttcaccaa ggctggatcg tgggacaccg agatcaagac ccgcgtcgcg
    14761 ggtgggaacc cgccggacgt gggcctcttc ccgcagcccg gcgtgatgtg cgacctggcc
    14821 aagcagggca aggtcctcgc ctatgacgac gcaaccgtca cctccgccaa ggccaccctg
    14881 gtcaacggct tcgtcggcgc cggcacctgc accgacggca aggtctacgg cctgccctcc
    14941 gcggtcagcg tcaagtcgct gctgtggtac gacgcgccag tcttcgccgc cgccggctac
    15001 aaggccccga cgacccttgc cgagctcgac gccctgaccg agaagatcaa ggccgacggc
    15061 aagaccccgt ggtgcatcgc cgctgagtcc ggtggcgcca ccggttggcc gatcaccgac
    15121 tggatcgagg acctcgtcct gcgcttcgcc ggcccggaga actacgacaa gtgggtcgct
    15181 ggcacgctga agttcaacag ccccgagatc aagccggcct gggactactt ccagaagatc
    15241 gccttcaccg agggcaacgt ccgcggcggc accaaggcca tcgcggcgac gaacttccag
    15301 accggtggca acgtcctgtt cgacaccccc ccggcgtgcc acctgttcaa gcaggccacg
    15361 ttcatcgcgg gcaagggtgg cttccctgac accgtcctgg ccaaggctga caccgagatc
    15421 ggcgtcgtcg ccttcccggc caaggaggct ggcaacaacc cggtcctgac cggtggcgac
    15481 ctggcgaccg cgttcaacaa cgacgccaac acgctcaagc tgcgcaactt catcgccagc
    15541 aaggacaacg gcgtcgaggt cggcaaggcc ggctacttct cgccgcacaa gtccttcgac
    15601 gtcacgctct acccgagcaa gatgctgcag aagatcgcca gcgacgttct ctacaagtcg
    15661 agcgccgctc gtttcgacgg gtcggacctc atgccggcca aggtcggcgc agggaccttc
    15721 tggaccgagc ccatcaagtg gatctccggt actcaggacc tcgacgtcac gctgaacaac
    15781 atcgacgcga gctggccgaa ggccgctagc tgaggtgaca ggacagctgc ggggatgaca
    15841 gcacgtcatc cccgcagctg atccatactt cccgccccat cactccttcg tcagggaggt
    15901 cagggactct tgaccatcaa aattctgaac gccttgctcg ccatcatcgg cggcatcggc
    15961 ggcgccatga tcttgttctg gttgctcaac aaacttgccg aatcgctcaa aggccgctgg
    16021 gaagaccggg tcaagccgtg ggtcttcgcc ggccccgcga tcctcgccat cggcgtctac
    16081 ctcatctacc cggccatcgt cacgctgcag tactcgttcg ccaacgagga cgcgacgaaa
    16141 tacgtcggct tcaacaacta caaagacctg ttgaccgacg ccacgttcct ctcggtgctg
    16201 ttcaacaacc tcttgtggat catcatcgtt ccggccctca ccgtggctct cggcctcggc
    16261 gttgcggtcc tcgccgaccg gctccggccc aagggtgaga agctctccaa gacggtcatc
    16321 ttccttccga tggccatcag catggtcggt gccgccacga tctggcgctc gatctacgag
    16381 taccagccga agggcagccc gcagaccggt ctgctcaacg ccatcatggg cgtgttcggc
    16441 aaggacccgg tgtactggta ccagatcgag acgtggcacc tcaacagcct gctgttgatg
    16501 gtcatcctca tctggaccca ggtgggctac tccatggtgt tgctctcctc ggccatcaag
    16561 ggcgttcctg aggacaccat cgaggctggc cggatcgacg gcgccggtga aaggcgcatc
    16621 ttcttcagca tcgtcgttcc ccagatctgg ccgaccgtgg tcacggtgtt catcaccgtc
    16681 ctcatcggtg tcatgaaggt cttcgacgtc gtgtacgtca cgacgaacgg tgcgttcaac
    16741 accgacgtcg tgggtcggcg gttcttctac gagatgttca ccaaccagaa cagcgggtat
    16801 gccgcggcca tcgtcgtgct cctgatgatc gccatcaccc ccgtgctcgt ttaccaggtg
    16861 aggcagttcc gggctgagga ggccaaccga tgaccaccgc agttccgccg agcaccgacg
    16921 ctcaagtcat tcgtcagagc aaggccggca gcggcccagg cagtgcgacc tggccggtgc
    16981 ggatcttcgt caccttcctc tgtctcatct ggctgatccc cgtcctgggt ctgctcatca
    17041 gctcgttccg cacgcgcgac gcccagttca actccggttg gtggacggcg atcgccaacc
    17101 cgctggaatt cacccagtgg accgtcaaca actacgccca ggtgctgacg acgaacgact
    17161 ccggcatcaa catgggccag gcgttcatca acagcttcat cgtcacggtg ccggcaacgg
    17221 tgatcccgat cctcatcgcg gcgtttgcgg cctatgcctt caccttcatg gagtggaagg
    17281 gacgcgacct ggtcttcgtc atcatcgtcg gcctgctcgt cgtccccaac caggtggccc
    17341 tcgtgcccct gctgaagctc tacagcagcc tgggcctcaa cggcaccttc ctcgcggtgt
    17401 ggctcgccca catcggcttc ggtatgccgt tggccgtcta catcttgcgc aactacatgt
    17461 cgtccttgcc caaggccgtc atcgagtcgg cgaagatcga cggtgccagc cacttcacga
    17521 cgttctggcg gctcatcatc ccgatgtcca ccccggcgct ggcttcgttc gcgatcttcc
    17581 agttcctctg ggtgtggaac gacctgctca tcgctctgct cttcctcggc cgtggtgaga
    17641 acaccatcgc gaccgtggcc ctgcagggct tgcaaggtca gacgggtcag ggccgtgagc
    17701 tcatcccgtc ggcgggcttc atcaccatca ttgtcccgct gatggtcttc ctgtcgctgc
    17761 agcggtactt catccgcggt atgacctccg gcgccgtcaa gggctgaggt cacccgcacc
    17821 atcgaggtcg agcccgctcc cccactgtgg ggtgcgggct cgacttcttc gagagcttcg
    17881 tgaaggacga tgacgacgcc gaaccatccg acagtgctgc cgacacgcag cctgttccgc
    17941 gccaccgacc gcgtagccgt cgagatccgc gacctgcgtc agtcggcaga gctgacggtg
    18001 caccgcgacg gcgccgtcct ttccagccac cccgtctcga gtgctggtgt cattcctttg
    18061 ggtgagttgg agccggggtc ctacggcatc tcgctccggg ccggcgacca gcagatcgct
    18121 cgctccgcgg tgcagatcgc cgggtctggt ggcgaccgag gcatcctgcg ctacgcgttc
    18181 gtcgccgact atcgccccga ccgcagcgtg gccgacctgg ccgccgtcgc cgacaatctc
    18241 cgtcgtctgc acatcaccgg cgtccagttc tacgactggg cctaccgcca cgccgacctg
    18301 ctcggcggtg gcgaacgtta cgccgacgcc ctgggtagcc ccgtcgacct cggcacggtc
    18361 cgcgccctga tc
//
LOCUS       DAOMZY010000019        18035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_2763_length_18035_cov_6.297553, whole genome shotgun sequence.
ACCESSION   DAOMZY010000019 DAOMZY010000000
VERSION     DAOMZY010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 18035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18035
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2763_length_18035_cov_6.297553"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..89
                     /locus_tag="PLK64_02220"
     tRNA            11..89
                     /locus_tag="PLK64_02220"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44..46,aa:Thr,seq:tgt)
     gene            complement(224..1708)
                     /locus_tag="PLK64_02225"
     CDS             complement(224..1708)
                     /locus_tag="PLK64_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPV78586.1"
                     /translation="MTDTHSHPSPSGIPLGGGCDPALALGEVQRRWRSDLLDQMGDFI
                     RIPALSPAFDADWVEHGFLDQMVELAAAWLTAQRLPGAVIEILRSPGRTPLLFVDIPA
                     QGTAAEATVLVYGHLDKQPEFQGWREGTGPWTPVFDGIRLFGRGSADDAYAVCLLGAA
                     LGALVAQGASYPRCVAVFETGEESGSPDLAYWLGVLGDRLGQVGLVACLDSGAGDYDR
                     LWAVTSLRGTASATLTVRVLDEGAHSGDAGGVVPSSFRVLRALLDRVESSATGEILLP
                     ELRCDIPADREDQARATAELLGASLWERFSWASSADGRVRACTEDPHEALLRRAWHPS
                     LAVVGAEGMPPLATAGNVLRPFTSIRLSLRLPPLCGAAAALAAVGEVLTADPPYGAVV
                     TFEPSPQVVDGWNAPPFSVELSTALNAASAACFDGAAPAFIGQGGTIPLMGLLGGLFP
                     DAQFLACGVQGPGTNAHGPNESLHIPYAERLTASLAVVINALAL"
     gene            complement(1705..2778)
                     /locus_tag="PLK64_02230"
     CDS             complement(1705..2778)
                     /locus_tag="PLK64_02230"
                     /inference="COORDINATES: protein motif:HMM:NF017812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 family protein"
                     /protein_id="HPV78587.1"
                     /translation="MKRTLTPDDIEAAVYGGAILGGGGGGFIEPGLRTARLALQVGSP
                     QLWSVDEFDEDVLSATVALVGAPAAPNALVQPAHLIRALDLLQSQLPGGRVAAINTNE
                     NGAETTVNGWFHSALTGVPVIDLACNGRAHPSSVMGSMGLHLDPDYTSIQAYAGGHPE
                     FYVEGVTKGRMEATASVVRRASVEADGWVGVARNPVTVGYAASNGAAGAISFAIELGR
                     VFLDGGVQAVTERLGGRIIASGTVGSYSCEPVEGLDIGKVVLTDAAVTTLLFVNEYMV
                     AEHNGERVGAFPDLIMTFDENGVPLPSARVQPGQTISVLVAPASSLKLASTMFMADLY
                     EPLEKALGVQFAPRSGVLAGAPA"
     gene            complement(2820..4511)
                     /locus_tag="PLK64_02235"
     CDS             complement(2820..4511)
                     /locus_tag="PLK64_02235"
                     /inference="COORDINATES: protein motif:HMM:NF015148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HPV78588.1"
                     /translation="MAHDTSATTDQVTERHPRALAPANLILTVLLSVFGALIGIQIIA
                     TLGITPSTSLLGALVAMTLSRIPLTIFRGFRNIHAQNLAQTSISSATFGAANSLFLPI
                     GIPFLFGQPSMIMPMLIGVAAAMLLDAWLLYRMFDTPAFPARRPWPLGIAAAEAIKAG
                     DQGGAQAKVLFGGIGVGTLGAIFSLPMSAFGVAMIGGFGAMMAFGIGLLTRAYAKPVA
                     QIDLMALYIPHGMMIGAGLVALVQVGRVVVSAHRNGDKDMDDEPEPTSTGSKASVSSA
                     GSSATSTAVATADPVNAASRLGQSLRVGGIGYLIIAALLAFGTGVFTQMSTAMLISFV
                     LYATVAAFVHELIVGIAAMHSGWFPAFAVALITLIIGIMIGFPPAALVVLAGFTAATG
                     PAFADMGYDLKAGYVLRGGGIDPTFELDGRRQQLIAAMVGFGVAIIAVAAAYPVFFGR
                     NMTAPIDAAYIAAIKAGVSGETARSLVIWAIPGAIIQFLGGSRRQLGVLFATGLLIST
                     PLAGWMVAGGLVVRIVASKVLGPVARERLEVFAGGVIAGDALYSFFSGAVKSLRS"
     gene            4719..5489
                     /locus_tag="PLK64_02240"
     CDS             4719..5489
                     /locus_tag="PLK64_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPV78589.1"
                     /translation="MSTLANARDVLRLLASHPRDLTVTDVAAGLDAPKSSVSRTLGMM
                     AEFGFLERDPQTLAYRPGPLILEAASHVRGSTDVVALLNEALVRLVDTTGYTGYLNLL
                     EGGETLVIRMRQGRAGRLQAYTPEGTRGTAYASSSGRAILARLTDEDVSGLIGTRLEP
                     AGSAPRTLPELLERLAQVRAEGWSLSRGESVHGVAGVASAVVDPTSGRPYGIGIGLPV
                     QDLETTDPQLLGEAVRDAAHSVGRKVADPFWLGFPTTN"
     gene            complement(5536..6210)
                     /locus_tag="PLK64_02245"
     CDS             complement(5536..6210)
                     /locus_tag="PLK64_02245"
                     /inference="COORDINATES: protein motif:HMM:NF007788.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HPV78590.1"
                     /translation="MNVAFLTIGQSPRHDMADPIEESLPAGTTVVHSGLLDGLSRPEI
                     ESAYGDGGAGQGLITRLADGSSVTMRVEAVEEGLRTKVASLTEGGVDAIVLLCTGEFP
                     SLRSGDVWLIEPDTVLTGYVVSLFAQVRVGMILPMAGQVDDARVKWRGLADPIFAFAS
                     PYSDDVEALVAAAYDLVDAGARALVLDCMGFSRRHREALRARGVEVPVLVANEVVGGV
                     VRIAFS"
     gene            complement(6238..7014)
                     /locus_tag="PLK64_02250"
     CDS             complement(6238..7014)
                     /locus_tag="PLK64_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HPV78591.1"
                     /translation="MTSDSHGPDVSDRQAHAEATFTRLFGPRDVNAPEDDPELMSILR
                     GFIFGDVFDTGVLDDRARELVTVTVLACLQTLPQLKAHTTAALHVGVDPIEIREAVYQ
                     LAPFIGFPRTLNAVATINEAFRAQGIELPLPDQGTVTDADRYARGFAEQEPLYGDEIK
                     ANLADLPEPFNEALPRFLTEFCFGDFYTRGGLSLAQRELLVLCALATIGDTSAQLGPH
                     ARACIQVGNSTAYVIAALVHCFPYIGFPRAVAAIRAVKSL"
     gene            7145..>7491
                     /locus_tag="PLK64_02255"
     CDS             7145..>7491
                     /locus_tag="PLK64_02255"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPV78592.1"
                     /translation="MDIQRFESNARMSQAVVYGGLVYFAGQVGAGETVAEQTTNVLAS
                     IDRLLAAAGSDRSRILTATIWLTDMATFGEMNGVWEQWVLPGSTPARATVEARLAAPA
                     YLVEISVTAAVAP"
     assembly_gap    7492..7591
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7853..9001
                     /locus_tag="PLK64_02260"
     CDS             7853..9001
                     /locus_tag="PLK64_02260"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HPV78593.1"
                     /translation="MAEAYIVSAVRTPVGRRGGGLAGVHPADLSAHVMRAAVETAGID
                     PAAVDDVVWGCVDQIGAQATNIARTGWLSAGLPESVPGTTIDRQCGSSQQAVSFAAYG
                     ILAGQQDLVLAGGVEAMSLVPIWSPATVGAAQGLGHPFGGQGWTARYGEAEVSQFYGA
                     ELIADRWDVTREDMERFALASHQRALAAQAAGAFADEIVPYAAVAADEGPRADTSLEK
                     MATLKTVRDGGRVTAAVSSQVSDGAAALLLASEDAVRTHGLTPLARIHALTVVGSDPI
                     VMLDGPIAATERVLARAGLTIGDIDQSEVNEAFAPVVLAWLKATGADPARTNPLGGAI
                     ALGHPLGATGARLMVTLVHALRRGGGRYGLQTMCEGIGMANATVIERC"
     gene            9064..10959
                     /locus_tag="PLK64_02265"
     CDS             9064..10959
                     /locus_tag="PLK64_02265"
                     /inference="COORDINATES: protein motif:HMM:NF008883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase"
                     /protein_id="HPV78594.1"
                     /translation="MDAQRLAETARGWVGSAARRAREAAGAVTGSGPDIQELLADPGL
                     AASLERYAAEHDLPIGPVRAEAAEHLHEMVATHNPRATQSWDKLGAWIMRAHDVLVDE
                     EDMARLKALDREHSLAIVFSHRSYLDGWVLPNVMSSRRFSPLFTFGGANLDLPVVGTL
                     VSRTGIIFIKRETKEMPVYRLTLRGYISHLVQRRSNLAWSIEGGRTRTGKLRPPVHGI
                     LRYLSDAAEASDGPDVMLVPVSIVYDQLHEVAGMTAEARGSRKRPEDLGWLIRFARSQ
                     GGRLGRAYVSIGEPFPLRQRMATLRAEGNDTSQAVERVAIDASHRINRATPVTTVAVV
                     CLALLGADRALTFERVLDTVEPLARYIRDRRWPVAGAANLTDRSTIRRALQELVASGV
                     LTVFEAGTEPVWRIAPDQHLVAAFYRNTVIHILVDRAIGEVALLDAIAAGEGANVERA
                     AWERAKALRDLLKFEFFFPGRDDFERELRAELALMAPGGAGALTLDSARALLDGSDLY
                     VANLVLRPFVDAYLVVADRLAAAGDSAVNEADVLDEALRVGQQWELERRIASAESVSL
                     ELYRTGLRLARHRGLLGGEGADTAYPGESLGARRAAFLVELQDVATQLDTIARITQAS
                     RSARGLR"
     gene            10946..12370
                     /locus_tag="PLK64_02270"
     CDS             10946..12370
                     /locus_tag="PLK64_02270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HPV78595.1"
                     /translation="MDFAEQLARIETAPQGASVGAFFDLDGTLVSGYTASTFFTDRVK
                     SREVDLGHFMRTFVAVIDGTYLGGEPTRGAIKGYAAMRGQSEETMTELGERLFVQKIA
                     GTIRPEARELVRAHQRRGHTVVIASAASKYQIQPVATDLGIHHLVCTQLAVEDGILTG
                     ELDGRLLWGTQKASGVRSFARSHDIDLKASFAYGNGAEDVAFLGAVGSPIALNPHPGL
                     RGAAKTFGWPVVMLRDPSGVSPTDMVRTVAALSGLNAGAAAGVAWGLVSGNRQRGINV
                     GIGLACQSALRLAGVHLRVVGEEKIPDGPAVYVANHQSSVDPLVAATLIHGEFTVVAK
                     KEARFDPRSLVGSLLLEPAYIDRSNSEQSRATLDALVSRIRGGTSLLIFPEGTRSTTQ
                     VLGRFRKGGFHLAIQAGVPVVPIVLRNTGEILPRHGRVLRPGSVDIAVLDPIDDWTVE
                     DLDAQVTALRDRFAATLADWPGAR"
     gene            12445..13815
                     /locus_tag="PLK64_02275"
     CDS             12445..13815
                     /locus_tag="PLK64_02275"
                     /inference="COORDINATES: protein motif:HMM:NF014998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HPV78596.1"
                     /translation="MNELETTMWRSERHPQQSSTICSLLILDTVPEWDRLVAAHEWAT
                     SLVPRSRQRVVEPIVPTGPPVWRTDPHFALTYHLRRMHVGPDGSMADLMELVQTLAMA
                     PFDRTRPLWEGTLIEGLEGGRAAYLLKLHHSLTDGLGAIQLMSLVQSRTREHTPDKPT
                     APEPDPYDVPPSDDALSVTVEGARHTVGQLPGLALAAADAGISLARNPGGAVAETLRF
                     AASLRRVLSPPPAPPSPLLRNRDGRVWVMRTLECPLADLRAAAKRAGGSVNDAYLAAL
                     LGGLRRYHEHHGVEIDELPITVPVSLRRSDDPMGGNKFAGAMLAAPIGIVDPAERVAA
                     LRGVILASLAEPALDSFSVITPVVNRLPSAVGAAVMGLGAATDLSASNMPGLPYEVFM
                     AGARVEQVYPFGPLPGVAMMAAMMSHVGTCCIGMTIDGTAVADVDVLMRCMQDGLDEV
                     LAVATS"
     gene            13873..14820
                     /locus_tag="PLK64_02280"
     CDS             13873..14820
                     /locus_tag="PLK64_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HPV78597.1"
                     /translation="MDTTTFTVTATDGTPLHVFRWLPDAGAAPKAVVQIAHGMAEHSA
                     RYARFAEVLTAAGYAVYANDHRGHGQTAGDLKRAGFFADHDGWSTVVEDMYAVTHTIR
                     EEQPGLPIFLFGHSMGSMLSRTYAIRHGAELDGLILSGTGGDPGMLGKVGQVVALVEA
                     KVRGKGTASPLLDKLSFGNFNKPFEPARTPFEWLSRDPAEVDAYIADPWCGFVCTAGM
                     FQDLLSGIALINADSQVARIPQGLPIYLFSGAEDPVGDKTKGVQQVVDQFRRLCIKDV
                     FVRFYLGARHEVLNETNRDEVMADVVDWLDDHLSVTERA"
     gene            complement(14804..15328)
                     /locus_tag="PLK64_02285"
     CDS             complement(14804..15328)
                     /locus_tag="PLK64_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPV78598.1"
                     /translation="MSDHTSSREHATTGTMEGERAPDAPLPHTAFVNQWRSGDTLLTL
                     RELLDLAEEVGPTIARRAELGHTELKAIELLVRSPHGPVELARELGVTSAASSGIVDR
                     LEARGHVRREAHATDGRRTQVVLTESAREEVLGHLVPMFGSLAAMDATFTEAEKVIVQ
                     RYLRGAIDAIKRVL"
     gene            15389..17422
                     /locus_tag="PLK64_02290"
     CDS             15389..17422
                     /locus_tag="PLK64_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPV78599.1"
                     /translation="MTRLGQAIVDLIVHRRRSWLVALLLVLVAGGVIGAMKEATRTTS
                     ALDGLPIGAESTAVVEVLQTFPQKEGSSAVVLYTATNGTLDEPTIAALRTRLQGRIAG
                     PLVVSEDKSAAIGVVPVTATGNAGISAAVTSLRTDLKADPPAGVTVQVTGPAAVQADL
                     GAVFQGANTRLLLATAGVVALLLLITYRSPTLWLVPLIVVGIADRLAAVLATHVLKAT
                     GVAWDESTIGILSVLVFGAGTDYALLLISRYRDELRREPDRYAAMRAALRHTGEAVLL
                     SATTVVLGVLTLLLSVFPTTRGLGLASAVGIVVAAGYALVVLPAVLVLFGRWVFWPVV
                     PRVGQTALVDQNSFWRKVGDLVAARPQRFVVGTLVVLALMAGGLTQVRSGLSQTDQFL
                     SKPEAIAAAERLAQSFPAGNSDPLVVITEGDPAAVVAAAKSVPSVASATPGQRTDRYA
                     QVAVVLTPQPGTDAARTAVRDVRAAVHGIPGTIVGGTEARGVDAADAASRDRGVIFPI
                     IFVLVLLALLVLLRSVVAPIILVGTVILTFLAAVGASWWIFTKVFGFTALDVGAPLYT
                     FLFLVALGVDYNIFLVTRAREEAAVHGSRAGMLRALSATGGVITSAGVLLAAVFAVLG
                     VLPLVVLAQIGVIICVGVLLDTLVVRTVLVPAIALILGDRFWWPRRVAPVERA"
     gene            17419..>18035
                     /locus_tag="PLK64_02295"
     CDS             17419..>18035
                     /locus_tag="PLK64_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPV78600.1"
                     /translation="MSGDPASPGDLMTSAGRERVAVVGSGVSGLTAAYLLSRAHDVTL
                     FEADDRLGGHAHTHSVAGWAGPVAVDSGFIVFNDRTYPVLRRLFAELGVSGRPTEMSM
                     SVRDDRSGVEFAGGRGLPGFVARPGQLARGSYWRMLTGVRRFHRLARVFLEGSTDEDP
                     TTFGEFIRAAGFGPDFVRLYAVPVVACVWSTGGGHALDYPARYLFR"
BASE COUNT         2694 a         6265 c         6184 g         2792 t
ORIGIN      
        1 ccgaagccag ccgactgtgg cgcaattggc agcgcacctg tcttgtaaac aggggttagg
       61 ggttcaagtc ccgcgtcgac tcagtcgtga cgcatgacgg cgccgatggc ggcgtccctg
      121 ctgtcgttgg gacagcagcg gtcgcgccgt catgggcgcc gccacggtgc gcggccgcgc
      181 caaccacctc gacgtcagag cgccagggcc aactacctcg acgtcagagt gccagcgcgt
      241 tgatcaccac ggccaaggac gcggtcagtc gctcggcgta gggaatgtgg agtgattcgt
      301 tcggtccgtg ggcgttggtc cccggccctt gcacgccgca cgccaggaac tgggcgtcgg
      361 ggaacaggcc gcccaacagc cccatcaacg ggatcgtgcc gccctgcccg atgaaggcgg
      421 gcgcggcccc gtcgaagcac gcggcgctgg cagcgttcaa cgcagtcgac aactcgaccg
      481 agaagggcgg cgcgttccac ccatcgacaa cctgcgggct cggctcgaag gtcacgacgg
      541 caccgtaagg cgggtccgcc gtcagaacct ccccgaccgc cgccagagca gccgccgcac
      601 cacacaatgg cgggagtcgc aaagacagcc ggatcgacgt gaacggtcga agcacgttgc
      661 cggccgttgc cagcggcggc ataccctcag cacccaccac cgcgaggctc gggtgccacg
      721 cgcgacgcag caacgcctcg tggggatcct ccgtgcaggc ccgcaccctg ccgtccgcgg
      781 agctggccca ggagaaccgc tcccagagag aggcgcccag caactcggcc gtggcgcgcg
      841 cctgatcctc gcggtcagca gggatgtcac agcgcaactc gggcaacagg atctccccag
      901 tcgcgctgga ctccacccga tcaagcaggg cgcgcagcac ccggaacgac gacggcacga
      961 cgcccccggc atcgccggag tgagcgccct cgtccagcac ccggaccgtc agcgtggcac
     1021 tggcggttcc ccgcaacgag gtcaccgccc agaggcggtc atagtcgccg gctcccgagt
     1081 cgaggcacgc cacgaggccg acctgcccga gtcggtcgcc caggacgccc aaccaatagg
     1141 ccagatcggg gctcccggac tcctcacccg tctcgaagac ggcgacgcac cgggggtagc
     1201 ttgcgccctg cgccacgagg gcgccgagcg cggcgccgag gaggcacacg gcatacgcgt
     1261 catccgccga gccacgcccg aagaggcgta tgccgtcgaa caccggagtc cacgggccgg
     1321 tcccctcccg ccagccctgg aactccggtt gtttgtcgag gtggccgtag accaacacgg
     1381 tcgcctcggc ggcggtcccc tgcgcgggga tgtcgacgaa cagcagcggc gtgcgcccag
     1441 gggagcgcaa gatctcgatc acggcacccg ggaggcgctg cgccgtcaac caggctgcgg
     1501 cgagctccac catctggtcg aggaacccat gctcgaccca gtcggcatcg aacgccgggc
     1561 tcaacgccgg gatccggatg aagtcgccca tctggtccag caggtcggag cgccacctcc
     1621 gttgaacctc tcccaacgcc agcgctgggt cgcacccgcc cccgaggggt atgccgctcg
     1681 gggacgggtg cgagtgcgtg tccgtcacgc gggtgcccca gccaacacgc cactgcgggg
     1741 ggcgaactgc acccccaacg ccttctcaag cggctcgtag aggtcggcca tgaacatcgt
     1801 cgaagcgagc ttgagactcg acgcaggcgc cacaaggacg ctgatggtct ggcccggctg
     1861 cacccgcgcg gacggcaggg ggacgccgtt ctcgtcgaac gtcatgatca gatccgggaa
     1921 ggcgccgacg cgctcgccgt tgtgctcggc gaccatgtat tcgttgacga acagcaaggt
     1981 cgtgacggct gcgtccgtga ggaccacctt gccgatgtcg aggccctcca ccggctcaca
     2041 ggagtaggac ccgacggtgc ccgacgcgat gatgcggccg ccgagccgtt ccgtgacggc
     2101 ctggacgccg ccgtcgagga acacccgacc cagctcgatg gcgaaggaga tcgcccccgc
     2161 ggccccgttg ctggcggcat acccgacggt cactgggttg cgagcgacgc caacccatcc
     2221 gtcggcttcg accgaggccc gacgcacgac gctcgccgtg gcctccatcc gccccttggt
     2281 gacaccctcg acatagaact ccgggtgccc tccggcatag gcctggatcg aggtgtagtc
     2341 agggtcgagg tgcagcccca tcgagcccat tacgctcgac ggatgggcgc ggccgttgca
     2401 cgcgaggtcg atcaccggca cccccgtgag agcggagtgg aaccagccgt tgaccgttgt
     2461 ctcggcgccg ttctcgttcg tgttgatggc ggccacccgg cccccgggca gctgggactg
     2521 gagcaggtcc agcgcccgga tgaggtgagc gggttggacg agggcgttcg gggcagcggg
     2581 cgcgccgacc agcgcgacgg tggcgctgag cacgtcctcg tcgaactcat ccacactcca
     2641 cagctggggg ctgccgacct gcagggcgag ccgggcggtg cgcaggcccg gctcgatgaa
     2701 gccgccgcca cctccgccca ggatcgcccc gccgtagacg gcggcttcga tgtcgtcggg
     2761 agtgagggtg cgcttcatgg gagcctcctg ggtctgggtg agtgttgggg gatcaagcgt
     2821 taggagcgca gggacttcac tgccccggag aagaaggagt agagggcgtc tccagcgatg
     2881 acgccaccgg cgaagacttc gagccgctcc ctggcgacgg gccccagcac cttgctcgcg
     2941 acaatgcgca ccacgagacc gcccgcgacc atccagccgg cgagcggggt ggagatcagc
     3001 aggccggtag cgaacagcac gccgagttgg cgtcgcgacc cgccaaggaa ctggatgatc
     3061 gcgccgggga tcgcccagat caccaggctc cgcgccgtct caccggagac tccggccttg
     3121 atcgccgcga tgtatgccgc gtcgatcggc gcggtcatgt tgcggccgaa gaacaccggg
     3181 tatgccgccg cgacggcgat gatcgcgacg ccgaacccga ccatcgcagc gatcaactgc
     3241 tgacgacgcc catcgagctc gaacgtgggg tcgatgccgc cgccgcgcaa gacgtagcca
     3301 gccttgaggt cgtagcccat gtcggcgaag gccgggccgg tggccgcggt gaagccggcc
     3361 agcaccacga gggcggcggg cgggaacccg atcatgatgc cgatgatcaa ggtgatgagg
     3421 gcgaccgcga aggcggggaa ccaccccgag tgcatcgccg cgatgccgac gatcaattcg
     3481 tggacgaatg ccgcgacggt ggcgtagagg acgaagctga tgagcatcgc cgtgctcatc
     3541 tgggtgaaga cgccggtccc gaaagcaagg agcgcggcga tgatgaggta accgatgccg
     3601 ccgacacgca gcgactgtcc gagcctgctg gcggcgttga ccgggtcggc ggttgcgacc
     3661 gcggtcgacg ttgccgacga tcccgcagac gataccgagg cctttgaccc ggtggacgtg
     3721 ggctccggct cgtcgtccat gtccttgtcg ccgttgcggt gtgctgacac gacgacccgg
     3781 cccacctgca ccagcgccac cagaccggcg ccgatcatca tcccgtgggg gatgtagagc
     3841 gccatgaggt cgatctgggc gaccggcttg gcgtaggcac gcgtgagcag gccgatccca
     3901 aaggccatca tcgccccgaa cccgccgatc atggcgacgc cgaacgccga catgggcaac
     3961 gagaagatcg ctccgagggt gccgacgccg atgccgccga agaggacctt cgcctgcgcc
     4021 ccgccctggt cgccggcctt gatggcctcc gcggcagcga tgcccaaggg ccagggccga
     4081 cgggccggga aggccggggt gtcgaacatc cggtagagga gccaggcatc cagcaacatc
     4141 gccgcggcga cgccgatgag catcggcatg atcatgctcg gctgcccgaa gaggaacggt
     4201 atgccgatcg gcaggaagag gctgttggcc gcaccgaacg tcgctgaact gatgctggtc
     4261 tgggcgaggt tctgggcatg aatgttccgg aagccgcgga agatcgtgag cgggatcctc
     4321 gagagggtca tggccaccag cgcgccaagg agggacgtgc tgggggtgat tcctagggtg
     4381 gcgatgatct ggatgccgat cagcgccccg aacacgctga gcaggacggt gaggatcagg
     4441 ttcgcgggcg ccagggcccg cggatgacgc tctgtgactt ggtccgtcgt cgccgacgtg
     4501 tcgtgggcca tgtacgtact ccttgtccgg gggctctcct tgaggcgact cttcctgccg
     4561 tgccccagtc tcgttctact atctaggacc gggttcgaga atatggacat ggtgatggtc
     4621 gtcgtgacgc ttgtcaaggg tcggaggcag cggcccgtgt ggcggtgtgg ccgtccgtca
     4681 cgcccactac gatcgacaag accgcgaagg aggggcagat gagcacgctg gcgaacgcac
     4741 gcgacgtgct ccgcctgctg gccagccatc cccgcgacct cacggtgacc gacgtcgccg
     4801 ccggtctcga cgcacccaag agttccgtgt cacgaaccct cggcatgatg gccgaattcg
     4861 gcttcctgga acgggatccg cagactctgg cctaccgtcc gggccccctg atcctcgaag
     4921 ccgcgtcaca tgtccgcggt tctacggacg tcgtggccct gctcaatgaa gcgctggtcc
     4981 gcctcgtcga caccacgggt tacacggggt atctcaacct cttggaaggc ggtgagaccc
     5041 tcgtcatccg gatgcgtcag gggcgggccg gaaggctgca ggcctacacg cccgagggga
     5101 cgcgggggac ggcatacgcc tcgtccagtg gtcgcgcgat cctggcgcga ctgaccgacg
     5161 aggacgtgtc ggggctgatc ggaacgcgcc tggagccggc cggctccgcg ccgcgaaccc
     5221 ttccggagtt gctcgagcga ctcgcccagg tccgcgcgga ggggtggtcg ctgtcgcgtg
     5281 gcgagtcggt gcatggcgtg gccggcgtgg cctcggccgt cgtcgatccc acctccgggc
     5341 ggccctatgg catcgggatc ggcctcccgg tccaggacct cgagacgacc gacccgcagc
     5401 tgctcggcga ggccgttcgc gacgccgcgc actcggtcgg acgcaaggtc gccgatccgt
     5461 tctggctggg cttccccacc acaaactgag cggtgcctgg cgctcggcgc cactctttcg
     5521 acgccgtcca ggctttcagg agaaggcgat tcgcacgact ccgccgacga cctcgttcgc
     5581 caccagcacg ggcacctcca ccccgcgagc ccggagcgcc tcccgatgcc gtcggctgaa
     5641 gcccatgcag tccaggacca gcgcgcgggc gccagcatcg acgagatcgt atgccgcggc
     5701 gaccagcgcc tcgacgtcgt cggagtaggg cgacgcaaag gcgaagatgg ggtcggcgag
     5761 cccgcgccat ttcactcgcg cgtcgtcgac ctggccggcc atcgggagga tcatcccgac
     5821 ccggacctga gcaaacagac tgacgacata gccggtcaac accgtgtctg gctcgatcag
     5881 ccacacatcg ccggaacgga gcgacggaaa ctcgccggtg cacagcagga cgatcgcgtc
     5941 gacgccgccc tccgtgagcg aggcgacctt ggttcggagg ccttcctcca ctgcctcgac
     6001 gcgcatcgtc acgctgctgc cgtcggccag tcgggtgatg agcccctgac cggcgccgcc
     6061 gtcgccatac gccgactcga tctcgggccg actcagaccg tcgagcagcc cgctgtgcac
     6121 gaccgtcgtg cccgcaggaa gcgactcctc gatcgggtcg gccatgtcat gccgaggcga
     6181 ctggccgatg gtcaggaacg caacgttcat gagggcggtg tcctttgcga cggctggcta
     6241 gaggctcttg acggcgcgga tcgcggcgac ggcgcggggg aagccgatgt aggggaagca
     6301 gtggaccagg gccgcgatca cgtatgccgt gctgttgccg acctggatgc acgccctcgc
     6361 gtgcgggccg agttgggcgg aggtgtcacc gatcgttgcc agggcgcaca ggaccagcaa
     6421 ctcgcgctgg gccaggctca gcccgccccg cgtgtagaag tcgccgaagc agaactcggt
     6481 gaggaaccgg ggcagggcct cgttgaacgg ctcggggagg tcggccaggt tggccttgat
     6541 ctcgtctccg tagaggggct cctgctcggc aaatccccgt gcgtaacggt ccgcgtcggt
     6601 gacggtgccc tggtcgggca gcggcaactc gatgccctga gcccggaacg cctcgttgat
     6661 cgtcgccacg gcgttgaggg tgcgcgggaa gccgatgaac ggcgccagct gatagacggc
     6721 ttcgcggatc tcgatggggt caacgccgac gtgcagggcg gcggtcgtgt gcgccttgag
     6781 ctggggcagg gtctgcaggc aggcgaggac cgtgacggta acgagctcgc gggcgcggtc
     6841 gtcgaggacg cccgtgtcga agacgtcccc aaagatgaag ccgcgcagga tgctcatcag
     6901 ctcggggtcg tcctcggggg cattgacgtc gcgcggcccg aacagccggg tgaacgtcgc
     6961 ctcggcatgc gcctgacggt cagacacgtc gggaccgtgg gagtcggaag tcatgcgagc
     7021 acctttgtcc cgcgccccgc cccggtcaag tcaggccctg ctgcgagaga gaggtcgttc
     7081 gctcggtgcc gacggcatcg ttcgcgccgc acccgcgagg tcccgcgcct ggaaggatct
     7141 gcccatggac atccaacgct tcgagtcgaa cgcacgcatg agccaggccg tggtctacgg
     7201 cggcctcgtc tactttgccg ggcaggtcgg tgcgggggag acagtggcgg agcaaacgac
     7261 gaacgttctc gcgtcgatcg atcggctcct cgcggcggct ggcagcgacc ggtcgcggat
     7321 cctcaccgcc acgatctggc tgaccgatat ggcgaccttc ggcgagatga acggagtctg
     7381 ggagcagtgg gtcctgcccg gttccactcc cgcgcgggcc accgtggaag cgcggctcgc
     7441 ggcaccggca tacctcgtgg agatctcggt gacagcggcc gtcgcgccct gnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtagggacg ccgtcatggg cgccgccacg
     7621 gcgcgcgggg gtgaggccgc gcatgaagca gtcgcagggt gcgtgatcac gccacctggc
     7681 gcggcttcat gggctgcccc acccccgggt atgccgcgcg ccgcccgtcc tcacctccgc
     7741 cgccccgcgc tcgtggccac cttcgcgcca ccagacgtga gatcggtcat ggcgggcacc
     7801 tgcgccccca cctcacctcc ccgccggcca cggaacgtgc tttggtggag tcatggccga
     7861 ggcatacatc gtcagcgccg ttcgcacccc cgtgggccgt cgaggagggg gcctggccgg
     7921 agtccacccg gccgatctct cggcccacgt catgagggcc gccgtcgaga ccgctggcat
     7981 cgatccggcc gcagtcgacg acgtcgtctg ggggtgcgtc gatcagatcg gcgcccaggc
     8041 caccaacatc gcccgcaccg gctggctcag cgcagggctg cccgagtcgg tgcccggcac
     8101 gaccatcgac cgccagtgcg gctcgtcgca gcaggcggtg agcttcgcgg catacggaat
     8161 cctcgcggga caacaggatc tggtgctcgc gggcggggtt gaggcgatga gcctcgtccc
     8221 catctggagc cccgcgacgg tcggcgccgc gcagggactc gggcacccgt tcggtggcca
     8281 ggggtggacg gcgcgctatg gcgaggccga ggtgagccag ttctacggcg ccgaactcat
     8341 cgccgaccgc tgggacgtca cgcgtgagga catggagcgg ttcgcgctcg ccagtcacca
     8401 gcgcgcgctc gccgctcagg ccgccggagc gttcgccgac gagatcgtcc cgtatgccgc
     8461 cgtcgccgcc gatgaggggc cgcgcgccga cacgtcgctg gagaagatgg cgactctcaa
     8521 gaccgtccgc gacggtggcc gagtcaccgc ggccgtgtcc agccaggtct ccgacggcgc
     8581 cgccgctctg ctgctcgcct ctgaagacgc ggtccgcacc cacgggctca ccccgctggc
     8641 gcgcattcac gccctcacgg tcgtcggctc cgacccgatc gtcatgctcg acggcccgat
     8701 tgccgcgacc gagcgggtcc tggctcgcgc cggcctcacc atcggagaca tcgaccagtc
     8761 ggaggtcaac gaggccttcg cgcccgtcgt cctcgcctgg ctgaaggcga ccggcgccga
     8821 cccggctcgc accaacccgc ttggaggcgc gatcgcgctc ggccacccgc tcggagcgac
     8881 cggcgcgagg ctcatggtca ccctggtgca cgcccttcgt cgcggcggcg gccgctatgg
     8941 cctgcaaacg atgtgcgaag gcatcgggat ggccaacgcg accgtgatcg agcgctgctg
     9001 agcggtgatt ccggagacgg atagcggttg acccgaggcc aaccgggcgt tagacctgtc
     9061 ctcgtggatg cgcagcgact ggccgagacg gcccgaggat gggtcggttc agcggcgcga
     9121 cgcgcccgcg aggcagccgg ggcggtgacg ggttccggac ccgacatcca ggagttgctc
     9181 gccgacccgg ggctggccgc ctcgttggag aggtatgccg ccgagcacga cctgcccatc
     9241 ggcccggtcc gtgccgaagc ggccgagcat ctgcacgaga tggtcgcgac ccacaaccca
     9301 cgagccaccc agtcctggga caaactcggc gcctggatca tgcgagcaca cgatgtcctc
     9361 gtcgacgagg aggacatggc gcggctcaag gccctcgatc gggagcacag cctggcgatc
     9421 gtgttctcgc accgctccta cctcgacggc tgggtcctgc ccaacgtcat gtcctcgcgc
     9481 cggttctcgc cgttgttcac cttcggcgga gccaacctcg atctgccggt cgtcggcact
     9541 ctggtcagcc gcacggggat catcttcatc aagcgggaga ccaaggagat gccggtctac
     9601 cggctcaccc tgcgcggcta catctcccac ctcgtccagc gccggtccaa cctggcctgg
     9661 tcgatcgagg gcgggcgcac ccgcaccggc aaactccgtc cacccgtgca cggcatcctg
     9721 cgttatctct ccgatgctgc agaggcgagc gacgggcccg acgtcatgct cgtccccgtg
     9781 tcgatcgtct acgaccaact ccacgaggtc gccgggatga ccgcagaggc gcgcggcagc
     9841 cgcaagcgcc ccgaggacct cggctggctg atccgcttcg cccgctcgca gggtggccga
     9901 ctcggccggg cctatgtctc catcggtgag cccttcccgt tgcgtcagcg gatggcgacg
     9961 ctgcgcgccg aaggtaacga cacgtcgcag gccgtcgagc gggtcgccat cgacgccagc
    10021 caccggatca accgggcgac gcccgtgacg acggtggcgg tggtctgcct ggcgctcctc
    10081 ggggccgacc gggcgctcac cttcgagcgg gtgctcgaca ccgttgagcc gctggcgcgc
    10141 tatatccgcg accgccgctg gccagtcgcg ggtgcggcca acctcaccga ccgctcgacg
    10201 atccgtcggg ccctgcagga gctcgtcgcc tcaggggtgc tcacggtctt cgaggccggc
    10261 accgagccgg tctggcggat cgcccccgac cagcatctcg tcgccgcgtt ctatcgcaac
    10321 accgtcatcc acatcctcgt cgaccgggct atcggcgagg tcgcgctcct ggatgccatc
    10381 gccgccggcg agggcgccaa cgtcgaacgc gccgcctggg aacgggccaa ggcgctgcgt
    10441 gacctgctca agttcgagtt cttcttcccc ggccgcgacg acttcgagcg tgagttgcgc
    10501 gctgagctgg ccctcatggc tcccggcggg gccggggccc tgaccctcga ctcggctcgg
    10561 gcgcttcttg acggcagcga cctgtatgtc gccaatctcg tgctgagacc cttcgtcgac
    10621 gcctatctcg tggtcgccga tcgccttgcg gcagctggag attcagcggt caacgaggcc
    10681 gatgtgctcg acgaggctct ccgcgtcggc cagcagtggg agctcgaacg ccggatcgcc
    10741 agcgccgagt cggtgtcgct cgagctgtat cgcaccggtc tgcgcctggc ccgccaccgt
    10801 ggtttgctgg gtggcgaagg tgccgacacg gcatacccgg gcgaatcgct gggggcgcgg
    10861 cgggcggcct tcctcgtcga actccaagac gtggcaacgc agttggacac tatcgcccgc
    10921 atcactcagg ccagcaggag cgcccgtgga cttcgctgag cagctcgcgc ggatcgagac
    10981 cgcaccgcag ggggcgagtg tcggcgcctt cttcgacctc gacggcaccc tcgtctcggg
    11041 ctacaccgcg agcaccttct tcaccgatcg ggtgaagagc cgcgaggtcg acctggggca
    11101 cttcatgcgc accttcgtcg cggtcatcga cggcacgtac ctcggcgggg agccgacgcg
    11161 aggggccatc aaggggtatg ccgcgatgcg cggccagagc gaagagacga tgaccgagtt
    11221 gggcgagcgg ctcttcgtcc agaagatcgc ggggacgatc cgccccgagg ctcgtgaact
    11281 cgtgcgcgcc caccagcgtc ggggccacac ggtcgtcatc gcctcggcgg cgagtaagta
    11341 ccagatccag ccggtggcaa ccgatttggg catccaccac ctcgtctgca cccagttggc
    11401 cgtcgaggac ggcatcctta ccggcgagtt ggacgggcgc ctgttgtggg gcacccagaa
    11461 ggccagtggg gtgcggtcct tcgcccggtc ccatgacatc gatctcaagg cgagcttcgc
    11521 ctacggcaac ggtgccgagg atgtcgcgtt cctcggggct gtcgggtcgc cgatcgcgct
    11581 caacccgcat cccgggctgc gcggagcggc caagaccttc ggctggccgg tcgtcatgct
    11641 gcgcgacccg agtggcgtca gcccgaccga catggtgcgc acggttgctg ccctcagtgg
    11701 cctcaacgcc ggcgccgctg cgggggtggc ctggggcctg gtcagcggca atcgccagcg
    11761 agggatcaac gtcggcatcg gcctggcctg ccagagtgcg ttgcggctcg ccggcgtgca
    11821 cctgcgcgtc gtcggcgagg agaagatccc ggacgggccg gcggtctatg tcgccaacca
    11881 tcagagttcc gtcgaccccc tggtcgcggc gacgctcatc cacggtgagt tcaccgtcgt
    11941 ggccaagaag gaagcgcggt tcgacccgcg gagtctggtc ggcagcctgc tgttggaacc
    12001 ggcatacatc gaccggagca actccgagca gtcccgcgcg acgctcgatg ctcttgtctc
    12061 ccggatccgg ggcgggacgt cgctgctcat cttccccgag ggcacccgct cgacgacgca
    12121 ggtgctcggc cgcttccgca agggcggttt ccatctggcg atccaagcgg gggtgcctgt
    12181 cgtgccgatc gtccttcgca acaccggcga gatcctgccg cggcacggcc gggtgcttcg
    12241 gcctggcagc gtcgatatcg cggttctcga tccgatcgac gactggactg tcgaggacct
    12301 cgacgcccag gtgactgcct tgcgtgaccg cttcgccgcg acgctggccg actggccggg
    12361 tgcccgatga acgccacccc gcgcggaccc ggttcctatc cggacgagcc tggcccggaa
    12421 cgggattggg cgcaggacga ccgcatgaac gagctcgaga cgacgatgtg gcgctccgag
    12481 cggcacccgc agcagtcctc gacgatctgc agcctgctca tcctggacac tgtgcccgag
    12541 tgggatcggc tggtcgccgc tcacgagtgg gccacgtcgc tggtgccacg ttcccggcaa
    12601 cgggtcgtgg agccgatcgt tcccaccggc ccgcccgtgt ggcggaccga tccgcacttc
    12661 gcgctgacct accacctgcg tcggatgcac gtcggcccgg acgggtcgat ggccgacctc
    12721 atggagctcg tgcagaccct ggcgatggcc ccgttcgatc gcacccgtcc cctctgggaa
    12781 gggacgctca tcgaggggct ggagggtggg cgcgcggcat acctgctcaa acttcaccac
    12841 tcactgaccg acggcctcgg cgccatccaa ctcatgtcgc tcgtccagag ccgcacccgc
    12901 gagcacacgc cggacaagcc gacggcaccc gagcccgacc cttatgacgt gccgcccagc
    12961 gacgatgcgc tgtcggtgac tgtggaaggc gcgcggcata ccgtcgggca gttgccgggc
    13021 ttggccctcg ccgcggctga tgcgggaatc tcgttggcgc gcaaccctgg tggcgcggtg
    13081 gccgagactc tgcggttcgc cgcgtcgttg cgccgggtgc tgtcgccacc gccggcccca
    13141 ccgtcgccgc tgctgcgcaa tcgcgacggt cgggtgtggg tcatgcgcac cctcgaatgt
    13201 ccgctggcag acctgcgggc cgcggccaaa cgcgccggag ggtcggtcaa cgacgcctac
    13261 cttgcggccc tgctcggcgg cctgcgccgc tatcacgagc accacggggt cgagatcgac
    13321 gagttgccca tcaccgtccc ggtgtcgctg cgacgcagcg acgacccgat gggcggcaac
    13381 aagttcgccg gagccatgct cgctgcgccg atcggcatcg tcgacccggc cgagcgggtt
    13441 gcggcgctgc gtggcgtgat ccttgcctcg ctcgcggagc ccgctctcga ctcgttctcg
    13501 gtcatcaccc cggtggtcaa ccggctgccg tcggcggtcg gcgctgcggt catggggctc
    13561 ggggcggcca ccgacctgtc ggcgtcgaac atgcccgggc tgccctatga ggtcttcatg
    13621 gccggtgccc gcgtggagca ggtctacccg ttcggcccgc tgccgggcgt cgcgatgatg
    13681 gcggcgatga tgtcgcacgt cggcacctgc tgtatcggga tgacgatcga cggaaccgcc
    13741 gtggccgatg tcgatgtgct catgcggtgc atgcaagatg gtctcgacga ggttctcgct
    13801 gtggcgacgt cgtgacgtcg tggcggcatg acggcgcagg accccgcgaa ctcgatgcct
    13861 gaggaggcac caatggacac caccacgttc accgtcacgg ccaccgacgg gacgccgctg
    13921 cacgtcttcc gctggttgcc tgacgcgggc gccgcgccca aggcggttgt ccagatcgcc
    13981 cacggcatgg ctgagcactc cgcgcgctac gcccggttcg ccgaggtgct caccgccgcc
    14041 gggtatgccg tctacgccaa cgaccaccgc ggccacgggc agaccgccgg cgacctcaag
    14101 cgggcggggt tcttcgcgga ccacgacggg tggtcgacgg tggtcgagga catgtatgcc
    14161 gtgacccaca ccatccgcga agagcagccg ggcctgccga tcttcctctt cggtcacagc
    14221 atgggctcga tgctcagccg gacctacgcc atccgccacg gcgcggagct tgacgggctg
    14281 atcctctcgg gcaccggcgg tgacccgggg atgctcggca aggtcggcca ggtcgtggcg
    14341 ctcgtcgagg ccaaggtccg cggcaagggc acggcgagcc cgctgctgga caagctgtcc
    14401 ttcggcaact tcaacaagcc gttcgagccg gcgcggacgc ccttcgagtg gctgtcccgt
    14461 gaccccgccg aggtggatgc ctacatcgcc gacccctggt gtggattcgt ctgcacggca
    14521 ggcatgttcc aggacctgct cagcggcatc gcgttgatca acgccgacag ccaggtcgcg
    14581 cggatcccgc agggtctgcc gatctacctg ttctccggcg ccgaggaccc ggtgggagac
    14641 aagaccaagg gcgtccagca ggtcgtcgac caattccgcc ggttgtgcat caaggatgtc
    14701 ttcgtgcggt tctacctcgg cgcccgccat gaggtgctca acgagaccaa ccgcgacgag
    14761 gtcatggctg acgtcgtcga ctggctcgac gaccacctga gcgtcacaga acgcgcttga
    14821 tcgcgtcgat cgcgccgcgc agatagcgct gcacgatcac cttctcggct tcggtaaagg
    14881 tcgcgtccat cgccgcgagg gagccgaaca tcgggacgag gtgcccgagc acctcctcgc
    14941 gcgctgactc ggtgagcacc acctgggtgc ggcgaccgtc ggtggcgtga gcctcccggc
    15001 ggacatggcc gcgggcttcg agccggtcaa cgatgccgga ggatgccgca gaggtgaccc
    15061 ccaactcgcg ggccaactcg accgggccat gcggtgaccg cacgagcaac tcgatcgcct
    15121 tgagctcggt gtgcccgagc tcggcgcgtc gggcgatggt cgggccgacc tcctcggcga
    15181 ggtcgagcaa ctcgcgcagg gtgaggaggg tgtcgccgct gcgccattga ttgacgaacg
    15241 ccgtgtgggg aagcggcgcg tccggcgccc gctccccttc catggtgccg gtcgtcgcat
    15301 gctcccggct tgacgtatga tcgctcatta gattactaat ctacttactt agttcgtgaa
    15361 gtaccagact agccgatcgg attgtcccat gactcgtctc ggtcaagcca ttgtcgacct
    15421 catcgtgcat cgacgtcgct cgtggctcgt ggccctgctc ctcgtgctcg ttgcgggtgg
    15481 cgtcatcggt gccatgaagg aggcgacccg cacgacgagc gcgctcgacg gattgccgat
    15541 cggcgccgag agcactgcgg tcgtcgaggt gctccagacc ttcccccaga aggagggcag
    15601 cagcgccgtc gttctctaca ccgcgaccaa cggcaccctg gacgagccga cgatcgccgc
    15661 cctgcgcacc cgcctgcaag gcaggatcgc cggtccgctg gtggtcagcg aggacaagag
    15721 cgccgcgatt ggggtcgtcc cggtcacggc gacgggcaac gccgggatct cggcggccgt
    15781 cacctccctg cgcaccgacc tcaaggccga cccgccggcg ggggtcaccg tccaggtcac
    15841 cggccccgcg gcggtccagg ccgacctcgg tgccgtcttc cagggcgcca acaccaggct
    15901 gctcctcgcc acggccggcg tggtcgccct cctgctgctc atcacctatc gcagcccgac
    15961 gctctggctc gtccccctca tcgtcgtcgg gatcgccgac cgcctcgccg cggtcctcgc
    16021 cacgcacgtc ctcaaggcca ccggcgtcgc gtgggacgag tcgacgatcg gcatcctctc
    16081 ggtcctcgtc ttcggcgcgg gcaccgacta cgccctcctt ctcatctcgc gttaccgcga
    16141 cgagttgcgc cgcgagcccg acaggtatgc cgcgatgcgc gcggctctgc ggcataccgg
    16201 agaagcggtc ctgctcagcg cgacgacggt cgtgctcggc gtcctcaccc tgctgttgtc
    16261 ggtcttcccg acgacgcgcg ggctcggtct ggccagcgcc gtcggcatcg tcgtcgcggc
    16321 cggatatgcc ctcgtcgttc tccccgcagt gctcgtgctc ttcggccgct gggtgttctg
    16381 gccggtcgtg ccgcgagtcg gtcaaacagc cctcgtcgac cagaactcgt tctggcgcaa
    16441 ggtcggcgac ctggtcgccg cgcggccgca gcggttcgtc gtcgggaccc tcgtcgtcct
    16501 ggcgctgatg gccggtgggc tcacgcaggt ccgcagtggc ctctcgcaga ccgaccagtt
    16561 cctctccaag ccggaggcga tcgcggctgc cgagcggttg gcccagtcct tcccggccgg
    16621 caactccgac ccgctcgtcg tcatcactga gggcgacccg gccgctgtcg tggcggccgc
    16681 caaatccgtt ccgtcggtcg cctccgcgac ccccggccag cgcaccgatc gctacgccca
    16741 ggtggccgtg gtcctcacgc cccagccagg gaccgacgcg gcgcgcaccg cggtgcggga
    16801 cgtgcgcgcg gccgtgcacg gcataccggg aacgatcgtc ggtggcacgg aggccagggg
    16861 tgtcgatgcc gccgacgccg cgtcgcgcga ccggggcgtc atcttcccga tcatcttcgt
    16921 cttggtgctg ctcgccctgc tcgtgctgct gcgttcggtc gttgcaccga tcatcctcgt
    16981 cgggacggtg atcctcacct tcctcgcggc cgtgggggcg tcctggtgga tcttcaccaa
    17041 ggtgttcggc ttcaccgcgt tggatgtcgg ggcgccgctc tatacgttcc tgttcctcgt
    17101 ggcgctcggc gtcgactaca acatcttcct cgtcactcgg gcgcgggagg aggcggctgt
    17161 tcacggttcg cgggcgggca tgttgcgggc gctctcggcc accggtggcg tcatcacgag
    17221 cgcgggcgtc ctgctggctg cggtgttcgc cgtcctcggg gtgctgccgt tggtcgtcct
    17281 cgctcagatc ggcgtcatca tctgcgtcgg cgtgctgctg gacaccctcg tcgtgcgcac
    17341 cgtcctcgtc ccggccatcg cgttgatcct cggcgaccgg ttctggtggc cgcggcgggt
    17401 ggctccggtg gagcgagcgt gagtggtgac ccggccagtc ccggcgatct catgacctcc
    17461 gcaggccggg agcgggtcgc ggtcgtcggc tccggggtct cggggctcac cgcggcatac
    17521 ctcctctccc gggcccacga cgtgaccctc ttcgaggccg atgatcggct gggcggacac
    17581 gcgcacacgc actcggtcgc cgggtgggca ggaccggtgg cggtcgactc gggcttcatc
    17641 gtcttcaacg accggaccta tcccgttctg cgtcggctct tcgccgagct cggggtcagc
    17701 gggcggccga ccgagatgtc gatgagcgtg cgtgacgatc ggtccggcgt cgaattcgcc
    17761 ggtggcagag ggcttccggg gtttgtcgcg cggccggggc agttggcgcg gggctcctat
    17821 tggcggatgc tgacgggggt ccggcggttc caccggctgg cccgggtgtt ccttgagggc
    17881 agcaccgacg aggatcccac gacgttcggt gagttcatcc gcgccgcggg tttcggtccc
    17941 gacttcgtcc gcctgtatgc cgtgcccgtc gtcgcgtgcg tctggtccac cgggggaggg
    18001 cacgcgctcg actacccggc ccgctacctg ttccg
//
LOCUS       DAOMZY010000020        17607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_2860_length_17607_cov_6.238320, whole genome shotgun sequence.
ACCESSION   DAOMZY010000020 DAOMZY010000000
VERSION     DAOMZY010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 17607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17607
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2860_length_17607_cov_6.238320"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PLK64_02300"
     CDS             <1..329
                     /locus_tag="PLK64_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPV78601.1"
                     /translation="ALGDEVRVTVIAAGFDGGDPTPRSDARALGQVQAGGPGRPAAAS
                     GPSTAATGGSSAGATYASGPAATPAPAPAPAPAPAPVVVPPVQVPPRPVSVDDADDLD
                     VPDFLK"
     gene            391..1161
                     /gene="pgeF"
                     /locus_tag="PLK64_02305"
     CDS             391..1161
                     /gene="pgeF"
                     /locus_tag="PLK64_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HPV78602.1"
                     /translation="MFFWHERRGGIDVAITDRHGGVSSAPFGELNLARHVGDEPAAVE
                     ANRVAVAAELGLPAERLVFMDQCHGATVEVIDGPWQGDPPACDAVITRASDLALVVMV
                     ADCVPVLLADPEAGVVGAVHAGRPGLVAGVVGAALDAMADLGARAPYAVVGPSVCGRC
                     YEVPEEMRAAAAAVEPASATVSWTGTPAIDVGAGVVAQLAGRGVPVTWVPGCTREDDR
                     LFSYRRTSPTGRFAGAIRRYAVDPAYLIERPSHPTETS"
     gene            1158..1943
                     /locus_tag="PLK64_02310"
     CDS             1158..1943
                     /locus_tag="PLK64_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate enzyme"
                     /protein_id="HPV78603.1"
                     /translation="MTGFSPQPTEVGDQRAEALRAGLADVRERVARACDAAGRDPAEV
                     TVIVVTKFFPASDVRRLAALGVRDMGENRDQEASAKFADVKADTAGLRLHFIGQIQTN
                     KAASVASYADVVHTVDRLKLVTALDRGAAAADRRLRVLVQVDLDPARPAATLGAVATA
                     AASAGPARGGADPADVGGLVAAVQASAALDLLGVMAVAPLGVDPGPAFGRLADVSRAV
                     RAVVPDATWISAGMSGDLEVAIRHGATHLRVGTAILGSRPTLR"
     gene            2023..2487
                     /gene="sepF"
                     /locus_tag="PLK64_02315"
     CDS             2023..2487
                     /gene="sepF"
                     /locus_tag="PLK64_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HPV78604.1"
                     /translation="MAGALRKTMVYLGLAEEDERYGSYDEAYEDDRVSEHTAAVTPLP
                     SRPTSVASVVRDPDMGSLTRITTIHPRTYNEAKAIGESFREGTPVIMNLTEMDDADAK
                     RLVDFAAGLVFGLHGAIERVTAKVFLLSPAHVEVTAEESPAVRSPRSPFNQS"
     gene            2552..2833
                     /locus_tag="PLK64_02320"
     CDS             2552..2833
                     /locus_tag="PLK64_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HPV78605.1"
                     /translation="MRGVWQVISTLLFIFFVFLIGRLVLDWVRVLARSWRPHGIVLVL
                     AEAVYTVTDPPLRLVRKIVPSITLGSIRIDLAFIILFLLTSTLMSLQPF"
     gene            2979..4271
                     /locus_tag="PLK64_02325"
     CDS             2979..4271
                     /locus_tag="PLK64_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HPV78606.1"
                     /translation="MALTPEQVLNKHFQTTQFRKGYDERDVDDFLDEIVAEMRSLLAQ
                     RDDYKQQLADCRAGRGMSITDAATVAAPAFVDTRAADEAEARLVEANAELAQAQTEHA
                     RVLESIGSARAEFDGLQSRLDGLRGSLADAQAQADGGPRLVQDAEAAVADAQRRAEEF
                     GSRAAEAEKHLYEIEARAAEADAKAQEAAARVSEAEQRAAEAQASAADAQARAEQAEA
                     AAQAAAERERQSIEATRVEAPAAPAAALVGAGATGAGSAAGLIELAQRLHDEHVAQGE
                     AKRHELVSAAENRHNELLATGQARFDELVGSGQRQHDDLVATAQSRHDELVSTAQNRH
                     DELVGTAQRQHDEMLAEAANLKEQMLTEARERSTGMVAEAQQKKAAVLEELEGQRTSM
                     ERKIDELRGFERTYRSQLRSYFEDKLSELTNTGADASA"
     gene            complement(4649..5068)
                     /locus_tag="PLK64_02330"
     CDS             complement(4649..5068)
                     /locus_tag="PLK64_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78607.1"
                     /translation="MTVEADRTADTALALAAFAGAALAGAAFAGAAFAGAAFVGAALA
                     GAALAGAALAGATLADTVFAGSDVTVATFAGAALVAAVLVVAFTGVAFAGVAFTAAAF
                     AGARLASVAFAGVAFTAAAFAAGLVLTTGAWPGAGSG"
     gene            5088..5477
                     /locus_tag="PLK64_02335"
     CDS             5088..5477
                     /locus_tag="PLK64_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HPV78608.1"
                     /translation="MPPVREDEEPWTEAELREVRQEIERDIARGLADIATAEEDLQGL
                     IWDAGDGSGDDQADAGAKTYEREQEISLANISRDKLAQNRHALERLDNGSYGICEACG
                     LPIGKFRLQAAPRATLCRSCKEKAERA"
     gene            5450..6073
                     /gene="lspA"
                     /locus_tag="PLK64_02340"
     CDS             5450..6073
                     /gene="lspA"
                     /locus_tag="PLK64_02340"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006548775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPV78609.1"
                     /translation="MQGESRARLSAPSPRPTRVSGSRRLIWLFVGIAVVVYVLDQASK
                     AWALAALTNAPPQDLLGAFLRLYLVRNPGAAFSVGTGSTWLLTILAVVVLVVLIRASR
                     RLGSMAWAWAFGLILGGALGNLTDRLIRDPGFGRGHVVDFIDYNGWFVGNVADIAIVG
                     AALLIGLLALRGIGLDGRRDGAGGSGEADGSGEADGIPEPRGTERDG"
     gene            6066..7004
                     /locus_tag="PLK64_02345"
     CDS             6066..7004
                     /locus_tag="PLK64_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPV78610.1"
                     /translation="MGDARTLFVPDGLDGERVDAAIARLFGFSRTRAAELAADGMVTL
                     NERVSPKSARVSAGDVLEVVIPDAPSAGVELVAEPVPGMSIVHDDDDLVVVDKPVGVA
                     AHPSVGWTGPNVVSGLAAAGYRIATSGASERQGIVSRLDVGTSGLMVVAKSEYAYSRL
                     KHAFRSRTVDKTYHALVQGLPDPMTGTVDAPIGRHPAHEFKFAVMASGKPAVTHYEVI
                     EAFHGASLLEIKLETGRTHQIRVHLSALHHPCCGDPLYGADPKVSARLGLDRQWLHAV
                     GLGFEHPGTGEWVTFASPYPADLEHALDVLADSPAR"
     gene            7144..10701
                     /gene="dnaE"
                     /locus_tag="PLK64_02350"
     CDS             7144..10701
                     /gene="dnaE"
                     /locus_tag="PLK64_02350"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPV78611.1"
                     /translation="MSTPPASERSFAHLHVHTEYSMLDGAARIDELLDAAVRMGMPAI
                     ATTDHGYVFGAYEFWSKARKRGIKPIIGVEAYLTPGTHRTDKTRVRFGDDPANKDDVG
                     GGGAYTHMTLLARTTGGMHNLFRMSSLASLEGHYFKPRMDRDLLSRFSGGLIATTGCV
                     GGEVQTKLRVGQYAAAREAAAELRDIFGAGNFYAEVMDHGLPIERQTQRELLRLAKDL
                     GLPLLATNDLHYTKAEDSTAHAALLCVQSGSTLMDPGRFKFDAEEFYLKSPAQMRELF
                     RELPEACDNTLLVAEQCDVSFTEEEGRYMPRFPCPEGESETTWFIKEVQRGLHKRYPG
                     GIPTAVQERADFEVEVILGKGYPGYFLVVADFINWAKDHGIRVGPGRGSGAGSMCAYA
                     MGITDLDPIVHGLLFERFLNPERPSMPDFDVDFDERRRGEVIRYVTDTYGEDRVAQIV
                     TYGTIKAKQAVKDAARVMGHPFAMGDRLTKVMPADVMGKSMPLSGIFDSSHARYAEAA
                     DFRKVHEEDPQAREVVTTALGLEGLKRQWGVHAAGVIMSSEPLADLIPIMRREQDGQI
                     ITQFDYPSCEKLGLVKMDFLGLRNLTILDDAIANIETNRGETIDLDVLSRTLDDKGTF
                     ELLARADTLGVFQLDGGGMRTLLRLMQPDNFEDISAALSLYRPGPMGANSHTNYALRK
                     TGKQPITPIHPELEAPLAEILGPTYGLIVYQEQVQHIAQRVAGYTLGKADMLRRAMGK
                     KKKEILDAEFVPFEAGMLANGYSAAAIKALWDVLVPFSDYAFNKSHTAAYGLVSYWTA
                     YLKANFPAEYMAALLTSVRDDKDKSALYLNECRHMGIKVLPPDVNSSVGNFAAVGSDI
                     RFGLEAIRNVGRNVVDSIIAARTEKGDFTSFEDFLRKCPATVLNKRTIESLVKAGAFD
                     SLGHQRAGLVRIHEAYVDALVDEKRQEAIGQDSLFGDFGFGGDDGESVQLVVLPPIPE
                     VEWDKATLLAFEREMLGLYVSDHPLFGIEHVLAAHADTSIASLMGDEPRPDGTQVTVA
                     GLVTNLQLKRTKKGDLWAIATVEDLDGAIECLFFPSSYQNVATRLVPDLVVSVRGRVN
                     ARDDTVSIYAQELNVPDLTDGPRGPVVLSMSLRQATGAGIERLRDILGQHPGVTEVHV
                     RLNQPGRSVLMRLDDTYRVTPSPALYGDLKALLGQGCLA"
     gene            10775..11929
                     /locus_tag="PLK64_02355"
     CDS             10775..11929
                     /locus_tag="PLK64_02355"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HPV78612.1"
                     /translation="MTPAQRRDLAVRRLAALRLDPLAPVAARTPLAVVEHHLAMQAQD
                     LSSGLWSIGVRTGGTVSDVLASVERGELTRTWPMRGTLHWVATADAGWLTALLAAPSM
                     RAAARVLEREGVIYEVVERAGRLWTEHLTGAGAMTRAEASAALTAGGVDPSGQRTYHL
                     LVRQHQLGLLRQGPIRRTGAGPASFEPTFVLAEGEADGAAGGCGSGSVRRPERAEALA
                     ILADRYVASHGPVTERDFAGWCDQPLRLVREAVAATEGRVRVETVGEAAYLVHEDAPP
                     AASEQTSPVLLLPGFDEWLLGYKDRSAQLTREQERAVVPGGNGVFRGTLVADSAVVGT
                     WRRAVARGTITVEVTPFTRPSRALRSGADEAAAAYGRFWELPCTLRWVDG"
     gene            11922..12947
                     /locus_tag="PLK64_02360"
     CDS             11922..12947
                     /locus_tag="PLK64_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPV78613.1"
                     /translation="MARGGIPPSPLPQREGVDAVRLRMPQGGPWALLRDHLIERIPLP
                     PHQIDAMLAAGEFVGPDGMPLAPDAPFVPRSVVWVHRDLPDEVPVPFKVTVLHHDERI
                     VVVDKPPFLATMPRGRHIRQTVLARLRVELGLPRLAPAHRLDRLTSGVLVLTTERRWR
                     GPYQQVFATGGADKDYLACAPLPKGGALPSGVVLPATVEVHLSKPHGQLQSHVLADRI
                     PNSRTDIDLIGRDEEAGVGLYHLRPHSGRTHQLRAHLAWLGIPICGDPLYPEIRDVDP
                     DDFQTPLGLVARRLRFRDPVDGTPRAFESDRVPWPWSGAAPTLAVAPVIDGSIPEVAS
                     PSGETKE"
     gene            13162..15270
                     /locus_tag="PLK64_02365"
     CDS             13162..15270
                     /locus_tag="PLK64_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPV78614.1"
                     /translation="MQPPRAPREPQSRIVHGHAVLDDYAWMADRDDPRLRAYLEAENA
                     YAAERTGHLDPLVERIVGELKSRTVETDLSVAVRHGGWWYYGRTIEGAEYPVHARVAI
                     SDSPDRPVLDGQTPPAHEQVLLDENVEAGDADFFAVGASEMSPDGRLLAWSADREGDE
                     RYDLVVREVDSGAILDATVRGIGDPLAWSLDNRHIFYTRLDESWRSHEIWRHEVGTAV
                     EADELVLAESDERFFLSVGATADDRWIVLSATSKTAAQVWLIDAADPRQSPRSVLPRR
                     DEVIYDVDPLADGLLVLHNAGRANFQVDWLPCAGAPVEDLVDLGWTTPDEFVTGMDAF
                     ATFVVLSLRVDGLTALRFVPVTGEGPGGFGMPHDLTFPGETGTLTLGSTPDPASPTVQ
                     VAHTSMATPPAIYDYDVRARTLLLLKAHPVPGVDLSALRETRIWATAPDGVAVPVSLV
                     HHADVRPDGTAAVLLYGYGAYGIPSDPWFSVARLSLLHRGVVFAIAHVRGGTELGWDW
                     YVQGRLEHKPHSFTDFLACADALVDQGWAAADRVAAEGGSAGGLLVAAAATQAPARFR
                     AVLAQVPFVDVLTTMLDPSLPLTVTEQQEWGDPIADPAAYDLIASYSPYETVPVGDFP
                     ALLITTSANDTRVFVTEPAKWMARLRDRVTDDPVCRPLLMRTQLTSGHGGRSGRYQAW
                     REIAWEWAVLLDLLGAADQS"
     gene            complement(15261..16043)
                     /locus_tag="PLK64_02370"
     CDS             complement(15261..16043)
                     /locus_tag="PLK64_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV78615.1"
                     /translation="MRIPTQGLVSVDTTADLVRLGLVLAVLVALAAAVSRVAGLGYSR
                     AQVTAALRATLQLALVGSVIAAVLDSWWLTAGFAALMLAVASVTAGRRVAPPGSRGWI
                     WGFVPICAGALPTATILLATGVVPLVPIAVVPVVGILVGGAMTATTLSGRRVADALRS
                     RHGEVEAALSLGLLDRDARLLVSRSDAGLALVPGLDQTRTVGLVTLPGAFVGMLLGGA
                     TPLQAAAVQLVVLVALLLVAVVATAITLELSARGTFAGPTQL"
     gene            16124..17488
                     /gene="hisD"
                     /locus_tag="PLK64_02375"
     CDS             16124..17488
                     /gene="hisD"
                     /locus_tag="PLK64_02375"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HPV78616.1"
                     /translation="MNRTDLRGSRLSRRTLREVLPRAEFDVAAAAEAVRPVVQDVAHR
                     GAAALRELSERFDGVVPPQLRVPADVLEAAVAQLDPALRAALEESIRRARIVHHDQRR
                     ADVTTRVVEGGTVTERWVPVERVGLYVPGGLAVYPSSVVMNVVPAQAAGVGSLAVTSP
                     PQRENVGVFAGYPNPTILATCALLGVDEVYAAGGAQAIAMLAYGFDETVTHADGSTET
                     LVCEPVSLITGPGSIWVTAAKRLVQGLVGIDAEAGPTEIAVLADDSADPEHVAADLLS
                     QAEHDPLAASVLVTVSEALADAVDAALARRVPDAKHGERIREALTGRQSRVVLVDDLD
                     AGILVCDVYAAEHLEIQTRDAAEVAGRIHNAGAIFVGPWSPVSLGDYAAGSNHVLPTA
                     GSAVHSSGLSVQSFLRGIHVVAYDEAALRGVAHHVVTLAQAEDLPGHGQAVTARFGDA
                     VPTP"
BASE COUNT         2626 a         6294 c         6071 g         2616 t
ORIGIN      
        1 acgccctggg cgacgaggtg cgcgtcaccg tcatcgccgc cggcttcgac ggcggcgacc
       61 cgacaccgcg ctcggacgcc cgcgcgcttg gccaggtgca ggccggtggc ccaggccgcc
      121 cggccgcggc ttcgggcccg agcacggctg cgaccggtgg ctcgtcggcg ggcgcgacat
      181 acgcctccgg tccggcggca accccggctc cggcgcctgc cccggctccg gcacctgccc
      241 cggtcgtcgt gccccccgtc caggtgcccc cgcgtccggt gagcgtcgac gacgcggacg
      301 acctggacgt gcccgacttc ctcaagtaac cgcagcgcgt cggtggtcga cacgtccgtg
      361 gtcgtcagcg tcccgcgtta gcgtggagcc gtgttcttct ggcacgaacg gcgcggcggt
      421 atcgacgtgg ccatcaccga ccgccacggc ggggtgagct cggcaccctt cggagagctc
      481 aacctggccc gtcacgtcgg tgacgaaccg gccgcggtcg aggcgaaccg cgtcgcggtg
      541 gccgccgagc ttggtctgcc cgctgagcga ctggtgttca tggaccagtg ccacggcgcc
      601 acggttgagg tcatcgacgg tccgtggcag ggcgatccgc ccgcgtgcga cgcggtgatc
      661 acgcgggcca gtgacctggc gctggtcgtc atggtggccg actgcgtccc cgtcctgctg
      721 gctgaccccg aggccggtgt cgtcggcgcc gtccacgccg ggcgtccagg tctggtcgcc
      781 ggtgtcgtcg gcgccgccct cgacgccatg gccgaccttg gcgcgcgcgc cccctacgcc
      841 gtcgtcggtc cgtcggtgtg cggtcgctgc tacgaggtcc ccgaggagat gcgggcggcg
      901 gcagccgcgg tcgagccggc cagcgccacc gtctcgtgga ccggcacgcc cgctatcgac
      961 gtcggcgccg gagtcgtcgc gcagttggcc ggtcgcggcg ttccggtgac gtgggtgccg
     1021 gggtgcaccc gcgaggacga ccggttgttc tcctaccgcc gcacctcacc gaccgggcgc
     1081 ttcgccgggg cgatccgcag gtatgccgtg gacccggcat acctgatcga gcgacccagc
     1141 caccccacgg agacgtcgtg accgggtttt cgccccagcc gaccgaggtg ggcgaccagc
     1201 gggccgaggc gttgcgggct gggctcgccg acgtgcggga gcgggttgct cgcgcctgtg
     1261 acgcggccgg gcgcgatccg gccgaggtca ccgtgatcgt ggtgacgaag ttcttccccg
     1321 cctccgacgt gcgtcggttg gccgccttgg gggtgcggga catgggggag aaccgcgacc
     1381 aggaggcgtc cgcgaagttc gccgacgtca aggccgacac ggcaggtctg cggctgcact
     1441 tcatcggcca gatccagacc aacaaggcgg cctcggtcgc gagctatgcc gatgtcgtcc
     1501 ataccgtcga ccggctcaaa ctcgtcacgg ccttggatcg aggggctgcc gcagccgatc
     1561 gacggctgcg cgtcctcgtt caggtcgacc tcgacccggc ccgccccgcg gcgacgttag
     1621 gagcggtggc gactgcggcg gcgtcagcgg gaccggctag gggaggcgcc gacccggcgg
     1681 acgtgggggg cctggtcgcc gcggtgcagg ccagcgccgc tctcgacctt ctcggtgtca
     1741 tggcggtcgc gcccttgggc gtcgatcctg gccccgcgtt cggcaggctc gccgacgtct
     1801 cccgcgcggt gcgcgcggtc gttcccgacg ccacctggat ctccgccggg atgagcggag
     1861 atcttgaggt ggcgatccgt catggcgcga cacacctgcg tgtcggcacg gcaatcctcg
     1921 gatcgcgccc gacccttcgc taacgtcgag ccaggcccgt atgccgcgca cccgctgcga
     1981 ctgctggctg agtagacatc cacggataaa ggggatcggc ccatggctgg ggcacttcgc
     2041 aagacgatgg tgtacctcgg gctcgccgag gaggacgagc gttacggctc ctacgacgag
     2101 gcctacgagg acgaccgcgt ctcggagcac acggctgccg tcacgccctt gccgtcccgg
     2161 ccgacctcgg tcgcgtcggt cgtgcgggac cccgacatgg ggtcgctcac ccggatcacg
     2221 acgatccacc cgcgcactta caacgaggct aaggcgatcg gcgagtcgtt ccgcgagggc
     2281 accccggtca tcatgaacct caccgagatg gacgacgccg acgccaagcg cctcgtcgac
     2341 ttcgcggccg gcctggtctt cggcctgcac ggcgccatcg aacgggtcac cgcgaaggtc
     2401 ttcctgctct cgccggccca cgtcgaggtc accgccgagg agtctccggc ggtccgttcg
     2461 ccgcggagcc cgttcaacca gagctgagcg gccgggccga gtcggcgcgg cggcataccc
     2521 acctcacagc cagatcgggg acgatgggcc gatgcgcggc gtgtggcagg tcatctcgac
     2581 ccttctgttc atcttcttcg tcttcctcat cggtcgactc gtgctcgact gggtccgggt
     2641 gctggcccgg tcctggcgac cccacgggat cgtcctcgtc ctggccgagg ccgtctacac
     2701 cgtcaccgat ccgcccctgc ggttggtccg caagatcgtc cccagcatca ccttggggtc
     2761 gatccgcatc gatctggcct tcatcattct cttcctgctc acgtccaccc tgatgagcct
     2821 gcaaccgttc tagcgcaggc gaattgggga ctttgagcag acgcaaccgc gcccgaggat
     2881 cgggccctta cgcgacgtcg gctaccgtcc ggttgggtca tcaggactcg attagataac
     2941 gttaggtcgc tactatccac tgtccgtcga ggtgaaccat ggcgctcacg cccgaacaag
     3001 tgctgaacaa gcacttccag acgacccagt tccgtaaggg gtacgacgag cgcgacgtcg
     3061 acgatttcct cgatgaaatc gtcgccgaga tgcgttcgct gcttgcgcag cgggacgact
     3121 acaagcagca gttggccgac tgccgggctg gccgcgggat gtcgatcacc gacgccgcga
     3181 ccgttgcggc accagcgttt gtcgacactc gggcggccga cgaggccgaa gcccgactcg
     3241 tcgaggcgaa tgcggagttg gcccaggccc agaccgagca cgctcgggtc ctggaatcga
     3301 tcggcagcgc gcgcgccgag ttcgatggcc tgcagagtcg gctcgatggg ctgcggggct
     3361 cgttggccga cgcccaggcc caggccgatg gtggcccccg gttggtccag gacgccgaag
     3421 ccgcggttgc cgacgcccag cgtcgcgccg aagagttcgg gtcgcgggcc gcagaggccg
     3481 agaagcacct ctacgagatc gaggctcgcg cggccgaggc cgacgccaag gcgcaggagg
     3541 ctgcggccag ggtcagcgag gcggagcagc gcgccgctga ggcacaggcg agcgccgccg
     3601 acgcgcaggc acgcgccgag caggctgagg cggcggccca ggctgcggcc gagcgtgagc
     3661 gtcagtccat cgaggcgacc cgggtcgagg cgccggcggc acccgccgcg gctctcgtcg
     3721 gtgccggtgc gacgggcgcg ggaagcgccg cgggcctcat cgagttggcc cagcggttgc
     3781 acgacgagca cgttgcccag ggcgaggcca agcgccacga gctggtctcg gccgccgaga
     3841 accgccacaa cgagctgctc gcgaccggcc aggcccggtt cgacgagctc gtcggcagcg
     3901 gccagcgtca gcacgacgac ctggtggcga cggctcagag ccggcacgac gagttggtct
     3961 cgaccgctca gaaccgccac gacgagctcg tcggcacggc ccagcgtcag cacgatgaga
     4021 tgcttgccga ggccgccaac ctcaaggagc agatgctcac cgaggcgcgc gagcgctcga
     4081 cgggcatggt cgccgaggcg cagcagaaga aggccgctgt ccttgaggag ctcgagggcc
     4141 agcgcacgtc gatggagcgc aagatcgacg aactgcgtgg gttcgagcgc acctaccgct
     4201 cgcagctgcg gtcctacttc gaggacaagc tctcggagct gaccaatacc ggagccgacg
     4261 cctccgcctg atcgcgtcac ccgcgtggcc gcggccggac ctagttccgg tatgccgcgc
     4321 agcaccgcct cgacgagggc ccggacctgt tccgggccct cgttcggtgc ccggtctgat
     4381 gatctaggtc accttcacgt acctagacgg gcgcggattg gcgccaaacc cccctgaaac
     4441 cgggcaggtg tcctattctc ggcgcgatcg tccatacacc catcgggcga gcggacaccg
     4501 gaaggggccg gacgaccatg gcggcgacga agggcaagaa cgccgcggct gctgccggtc
     4561 gagcgacggc aagcgtcgct cgagcggttc gggcggtgct gtcaggagca gtggctggca
     4621 cccggtcgag cgaagccgat ccatcgggtc accccgaccc agccccaggc cacgcacctg
     4681 tcgtcaagac caggccggcc gcgaaggcgg cggcagtgaa ggcaacaccc gcgaaggcga
     4741 cactggccaa gcgggcaccc gcgaaggcgg ctgcagtgaa ggcaacaccc gcgaaggcga
     4801 cacctgtgaa ggcgacaacc agaacggcgg cgaccaaggc tgccccggcg aaggtggcaa
     4861 ccgtgacgtc agaccccgcg aagactgtgt ccgccaaggt tgcacctgcc aaggccgcac
     4921 ctgccaaggc cgcacctgcc aaggccgcac ccacgaaggc tgcaccggcg aaggctgcac
     4981 cggcgaaggc cgcacctgcc aaggctgcac cggcgaaggc cgcgagcgcc aaggccgtgt
     5041 cggcggttcg gtccgcctcc acggtcatca gctctgtgtc agcggacatg cctcccgtac
     5101 gcgaggacga ggagccgtgg accgaagccg aattgcggga ggtgcgccag gagatcgagc
     5161 gggacatcgc tcgcgggcta gccgacatcg ccaccgccga ggaagacctc cagggcctca
     5221 tctgggatgc gggcgacggg tctggtgatg accaggccga cgcgggagcc aagacctacg
     5281 agcgcgagca ggagatctcg ctggccaata tctcccggga caagctggcc cagaaccggc
     5341 acgcgttgga gcggttggac aacggcagct acgggatctg cgaggcgtgc ggtctgccga
     5401 tcggcaagtt tcgactccag gccgctcccc gtgcgaccct atgcaggtca tgcaaggaga
     5461 aagcagagcg cgcctgagcg caccgtcccc ccgaccgacc cgagtttccg ggtcacgtcg
     5521 gctcatctgg ctattcgtcg ggatcgcggt cgtggtctac gtcctcgacc aagcgagcaa
     5581 ggcgtgggcc ctcgccgccc tcaccaacgc ccctccccag gacctcctcg gcgccttcct
     5641 gcgcctctac ctcgtccgta accccggggc tgccttctcg gtcgggaccg gtagcacgtg
     5701 gctgctcacc atcctggccg tcgtcgtcct cgtcgtcctc atccgggcca gtcgacggct
     5761 gggcagcatg gcctgggcct gggccttcgg cttgatcctc ggcggcgcgc tgggcaacct
     5821 gaccgatcgg ctgatccgcg acccgggctt cggccgcggc cacgtggtgg acttcatcga
     5881 ctacaacggc tggttcgtcg gcaacgtcgc cgacatcgcc atcgtcgggg ccgccctgct
     5941 gatcggcctg ctcgccctgc gaggcatcgg cctggatgga cgtcgggacg gcgcgggagg
     6001 atctggtgag gcggacggat ctggtgaggc ggacggcata cctgagccgc gggggacgga
     6061 acgcgatggg tgacgcgcgc accctgttcg ttccggacgg acttgacggt gagcgcgtcg
     6121 acgcggccat tgcccggttg tttgggttct cgcgcacgcg tgcggccgaa ctcgccgccg
     6181 acggcatggt caccctcaac gagagggtga gccccaagtc ggcccgggtg agcgcgggcg
     6241 acgtcctcga ggtcgtcatc ccggacgccc cgagtgcagg cgtcgaactc gtcgccgagc
     6301 cggtccccgg gatgagcatc gtccacgacg acgacgacct cgtcgtcgtc gacaaacccg
     6361 tcggcgtggc ggcgcacccg agcgtgggct ggaccggccc caacgtcgtc tcagggctcg
     6421 ctgcggctgg ctaccggatc gccacgtcgg gggccagcga gcggcagggc atcgtcagcc
     6481 gactcgatgt gggcacctcg gggctcatgg tggtcgccaa gagcgaatac gcctacagca
     6541 ggctcaagca tgccttccgt tcccggacgg tcgacaagac ctaccacgcg ctcgtccagg
     6601 ggctccctga cccgatgacg ggcaccgtgg acgcgccgat cggacgtcac ccggcgcacg
     6661 agttcaagtt cgccgtcatg gcctctggga agccggccgt cacccattac gaggtgatcg
     6721 aggctttcca tggggcctcg ctgctggaga tcaagctcga gacggggcga acccatcaga
     6781 tccgggtgca cctctcggcg ctgcaccacc cgtgctgcgg cgacccgctc tatggcgccg
     6841 accccaaggt gtcggcgcgc ctcggcctgg accggcagtg gctgcacgcc gtcgggctgg
     6901 gcttcgagca ccctgggacg ggtgagtggg tcaccttcgc cagcccctat ccggccgacc
     6961 tcgagcacgc cctggacgtg ttggccgact caccagcgcg ctgagacctg ccgggtcgag
     7021 gtattccgcc atcgtctgct gatagcggaa tacctcgacc ccgcaagggg tcgggggtgg
     7081 acgggggcga cacgggcggc gacacgcgcg gggtcgtcgg cggggcggca tacagttgct
     7141 gcgatgtcca ccccacccgc ctccgagcgc agcttcgcgc acctgcacgt ccacaccgag
     7201 tactccatgc tcgacggcgc cgcgcggatc gacgagttgc tcgacgcggc cgtgcggatg
     7261 ggtatgccgg ccatcgccac caccgaccac ggctacgttt tcggcgccta tgagttctgg
     7321 tccaaggccc gcaaacgggg gatcaagcca atcatcgggg tcgaggccta tctcacgccg
     7381 ggcacccacc gcaccgacaa gactcgggtc cggttcggcg acgacccggc caacaaggac
     7441 gacgtcggcg gcggcggcgc ctacacccac atgacgctgc tggcccgcac cactggaggc
     7501 atgcacaacc tcttccggat gagttccctg gcgtcactcg aaggccacta cttcaagcct
     7561 cggatggacc gcgacctcct ctcgaggttc tccggcgggc tcatcgcgac cacgggctgc
     7621 gtcggcggcg aggtccagac gaaactgcgc gtggggcagt atgccgcggc ccgcgaggcc
     7681 gccgcggagc tgcgggacat cttcggggct ggcaacttct atgccgaggt catggaccac
     7741 ggcctgccca tcgagcggca gacccaacgc gagttgttgc ggctggccaa ggacctcggg
     7801 ctgccgctgc tggcgaccaa cgacctgcac tacacgaagg cggaggactc caccgcgcac
     7861 gcggcgctgc tgtgtgtgca gtcggggtcg acgctcatgg acccggggcg gttcaagttc
     7921 gatgctgagg agttctacct caagtcgcct gcgcagatgc gcgagttgtt ccgtgagttg
     7981 cccgaggcgt gcgacaacac gttgctggtc gccgagcagt gtgacgtctc cttcaccgag
     8041 gaggagggtc gctacatgcc ccgattcccc tgtccggagg gggagtcgga gaccacctgg
     8101 ttcatcaagg aggtccagcg cgggctgcac aagcgttacc ccggcggcat cccgaccgcc
     8161 gtgcaggagc gggctgactt cgaggtcgag gtcatcctcg gcaagggtta cccgggctac
     8221 ttcctcgtcg tcgccgactt catcaactgg gccaaggacc atggcatccg ggtcgggccg
     8281 ggacgtggct ctggtgccgg ttcgatgtgc gcctacgcca tgggcatcac cgacctcgac
     8341 ccgatcgtcc acgggctgct gttcgagcgc ttcctcaacc ccgagcggcc gtcgatgccc
     8401 gacttcgacg tcgacttcga cgagcgtcgg cggggcgagg tcatccgcta cgtcaccgac
     8461 acctatggcg aggaccgggt cgcccagatc gtcacctacg gcaccatcaa ggccaagcag
     8521 gccgtcaagg acgccgcccg ggtcatgggc caccccttcg ccatgggcga ccggctgacc
     8581 aaggtcatgc cggccgacgt catgggcaag tcgatgccgc tgtcgggcat cttcgactcg
     8641 tcccatgccc ggtatgccga ggccgccgac ttccgcaagg tccacgaaga ggacccgcag
     8701 gcccgtgagg tcgtcaccac cgcgctcggc ctggagggcc tcaagcgcca gtggggtgtg
     8761 cacgcagccg gggtcatcat gtccagcgag ccgctggccg acctcatccc gatcatgcgc
     8821 cgcgagcagg acggccagat catcacgcag ttcgactacc cgagctgcga gaagctcggc
     8881 ctggtcaaga tggacttcct cggcctgcgc aacctcacca tcctcgatga cgccatcgcc
     8941 aacattgaga ccaaccgcgg cgagacgatc gacctcgacg tgctctcgcg caccctggac
     9001 gacaagggca ccttcgagtt gctggcccga gccgacaccc tcggggtgtt ccagctcgac
     9061 ggcggcggta tgcgcacgtt gctgcgcctc atgcagccgg acaacttcga ggacatctcc
     9121 gcggccctct cgctctaccg cccggggccg atgggcgcca acagccacac caactacgcc
     9181 ctgcgcaaga ccggcaaaca gccgatcacc ccgatccacc ccgagctcga ggcgccgctc
     9241 gcggagatcc tcggaccgac ctacggcctg atcgtctacc aggagcaggt ccagcacatc
     9301 gcccagcggg tggccggcta caccctgggc aaggccgaca tgctgcgccg agcgatgggc
     9361 aagaagaaga aggagatcct cgacgccgag ttcgtcccct tcgaggccgg catgctggcc
     9421 aacggctatt cggcggcggc catcaaggcg ctctgggacg tcctggtccc cttctcggac
     9481 tacgccttca acaagtcgca caccgcggca tacgggctcg tctcctattg gaccgcctat
     9541 ctcaaggcga acttcccggc cgagtacatg gccgccctgc tcaccagcgt ccgcgacgac
     9601 aaggacaagt cggcgctcta tctcaacgag tgccggcaca tgggcatcaa ggtcctgccg
     9661 cccgacgtca actcctcggt cggcaacttc gcggccgtcg gctccgacat ccgcttcggg
     9721 ctcgaggcga tccgcaacgt cgggcgcaac gtcgtcgact cgatcatcgc cgcgcgcacc
     9781 gagaaggggg acttcacctc gttcgaggac ttcctgcgta agtgccccgc caccgtcctc
     9841 aacaagcgca ccatcgagtc gctcgtcaag gcgggagcct tcgattccct cgggcaccag
     9901 cgcgccgggc ttgtgcgcat ccacgaggcc tacgtcgatg ccctcgtcga tgagaagcgg
     9961 caggaggcga tcggtcagga ctccctcttc ggcgacttcg gtttcggcgg cgatgacggt
    10021 gagagcgtcc agctcgtcgt gctcccgccg atccccgagg tcgagtggga caaggcgacc
    10081 ctgctggcct tcgagcggga gatgctcggc ctctatgtct ccgaccaccc gctgttcggc
    10141 atcgagcacg tcctggccgc gcacgccgac acctcgatcg cctcgctcat gggtgacgag
    10201 ccccgccctg acgggaccca agtcaccgtc gccgggctgg tgaccaacct gcagctcaag
    10261 cgcacgaaga agggcgacct ctgggcgatc gccacggtcg aggacctcga cggtgccatc
    10321 gagtgcctgt tcttcccgtc gtcctaccaa aacgtcgcca cccggctggt gcccgacctg
    10381 gtcgtgtcgg tccgcggccg ggtcaacgcc cgcgacgaca cggtgtcgat ctacgcccag
    10441 gagctcaacg tccccgacct caccgatggc ccgcgcggcc cggtcgtcct ctcgatgagc
    10501 ctgcgtcagg cgaccggcgc ggggatcgaa cggttgcggg acatcctcgg ccagcacccc
    10561 ggcgtcaccg aggtccacgt ccggctcaac cagcccggcc gctcggtcct catgcgcctg
    10621 gacgacacct accgcgtcac tccgtcgccc gctctctacg gcgacctcaa ggccctcctc
    10681 ggtcaagggt gcctcgcctg agcggcctgg tcgcggggcg gttcccgcgc acacggtccc
    10741 gggccgcgcg gcatacccga ggcaggggta tgccgtgacg ccggctcagc gtcgcgacct
    10801 cgctgtccgg cggctggccg cgctgcggct ggacccgctc gctccggtcg ctgctcgcac
    10861 tccgctcgcc gtggttgagc atcacctggc catgcaggcc caggacctgt ccagcggact
    10921 ctggtcgatc ggcgtgcgaa ccggcggcac tgtgtcggat gtccttgcct ccgtcgagcg
    10981 gggcgagctc acccgcacct ggccgatgcg cggcaccctg cactgggtgg ccaccgccga
    11041 cgccggctgg ttgaccgccc tgttggccgc cccgtcgatg cgggcggccg cacgggtgct
    11101 ggagcgcgaa ggcgtcattt acgaggtggt cgagcgagcc ggccggctgt ggaccgagca
    11161 cctcacgggc gccggagcga tgacccgggc cgaggcgtcc gccgcgctga ccgcgggtgg
    11221 cgtcgacccg agcggccaac gcacctatca cctcctggtc cgtcagcatc aactcggcct
    11281 gttgcgccaa gggccgatcc gccgcaccgg ggccggtcca gcctccttcg agcccacctt
    11341 cgtgctcgcc gagggcgagg ccgacggggc tgcagggggc tgcggttccg gctccgtgcg
    11401 ccgaccggag cgtgccgagg cgctcgccat cctcgccgat cgctatgtcg ccagccacgg
    11461 cccggtcacc gagcgtgact tcgccggctg gtgcgaccaa cctctgcggc tcgtccggga
    11521 ggcagtggcc gccaccgagg gacgggtgcg ggtggagacg gtgggggagg cggcatacct
    11581 cgtccacgag gacgccccgc ccgcggcgag cgagcagacg tccccggtgc tgctgctgcc
    11641 cgggttcgac gaatggttgc tcggttacaa ggaccgcagc gcccaactca cccgcgagca
    11701 ggagcgcgcg gtcgtccctg ggggcaacgg cgtgttccga gggaccctgg tggcggacag
    11761 tgcggtcgtc gggacctggc gacgtgccgt ggcccgcggc acgatcacgg tcgaggtgac
    11821 gcccttcacc cggccctcgc gcgccctgcg gtcgggagcg gacgaggcgg cggcggcata
    11881 cgggaggttc tgggagttgc cctgcacgct gcggtgggtc gatggctaga ggtgggatcc
    11941 cgccgtcccc gctgccgcaa cgtgaggggg tcgacgcggt ccggttgcga atgccccagg
    12001 gcgggccgtg ggctctgctt cgcgatcatc tgatcgagcg gatccccttg cctccccacc
    12061 agatcgacgc gatgctcgcc gcgggggagt tcgtcggccc cgacggtatg ccgctcgccc
    12121 cggacgcgcc gttcgtgccc cgatcggtgg tgtgggtcca ccgagacctg cccgacgagg
    12181 tgccggtgcc gttcaaggtg acggtcctgc accacgacga gcggatcgtc gtcgtcgaca
    12241 aaccgccgtt cctggccacg atgccgcgcg gccgccatat ccgtcagacc gtgctcgcgc
    12301 ggctgcgggt cgagttgggg ttgccgaggt tggccccggc tcatcgcctc gaccggctca
    12361 cctcgggggt gctcgtcctc accaccgaac gacggtggcg gggtccctat cagcaggtgt
    12421 tcgccacggg cggcgcggac aaggactacc tcgcctgtgc gccgctgccc aagggtggcg
    12481 ccctgccgtc cggcgtcgtg cttcccgcaa cggtcgaggt ccacctcagc aaaccgcacg
    12541 ggcaactgca atcgcacgtc ctggcggatc gaatccccaa cagccgcacc gatatcgacc
    12601 tcatcggccg agacgaggag gcgggcgtgg gcctctacca cctgcgccca cactcgggcc
    12661 ggacccacca acttcgcgcg cacctcgcgt ggctggggat cccgatctgc ggcgacccgc
    12721 tctaccccga gatccgcgat gtcgatccgg acgacttcca gaccccgttg ggcctcgttg
    12781 cccggcgcct gcgcttccga gacccggtcg atggaacacc ccgggcgttc gagtccgacc
    12841 gggtgccgtg gccgtggagc ggagcggcgc cgaccctcgc tgtcgcacct gtgattgatg
    12901 gatcaattcc tgaagtggct tcaccatcgg gtgagactaa ggaataatcc cctccgggag
    12961 gtatgaacct gggggctttc gggggtttac ctgtttgcca tggttcaaag gggcttcctc
    13021 gccgtttcgt cgattcgcct gtcgtgggga gacgacgcgt gggtcggtgc ggtggagggg
    13081 gaatagccgc ggtgggtggg tcgtgttgac acggcatacc cgccgcggtt ccccctgtcc
    13141 gacgctcact agagtgaccg gatgcaaccg ccccgtgccc cccgcgagcc gcagtcgcgc
    13201 atcgtgcacg ggcatgcggt cctcgacgac tacgcgtgga tggccgaccg cgacgatccc
    13261 cggctgcgcg cgtacctcga agccgagaac gcgtatgccg cggagcgcac cggccacctg
    13321 gacccgctcg tggagcggat cgtcggcgag ttgaagtcgc ggacggtgga gaccgacctg
    13381 tcggtggctg tccgccatgg cgggtggtgg tactacggcc gcaccatcga gggcgccgag
    13441 tatcccgtcc acgcccgggt cgcgatcagt gactcgccgg accgtcccgt ccttgatggc
    13501 cagacgcccc cggcacacga gcaggtcctt ctcgatgaga acgtcgaggc cggcgacgcc
    13561 gacttcttcg ccgtcggtgc gagcgagatg agcccggacg ggcgactgct cgcctggtcg
    13621 gccgatcgtg aaggggacga gcgctacgac ctggtcgtgc gggaggtcga ttccggcgcg
    13681 atcctcgatg ccaccgtccg ggggatcggc gacccgctgg cctggtcgct ggacaaccgc
    13741 cacatcttct acacgcggct ggacgagtcc tggcgcagcc acgagatctg gcggcacgag
    13801 gtgggcacgg cggtcgaggc cgacgagttg gtcctcgccg agtcggacga gcggttcttc
    13861 ctcagcgtgg gggccaccgc cgacgatcgg tggatcgtct tgagcgcgac ctccaagaca
    13921 gccgcccagg tgtggctcat cgatgcggcc gacccgaggc aatcaccgcg cagcgtcctg
    13981 ccccggcggg acgaggtcat ctacgacgtc gacccgctcg ccgacggcct gctcgtcctg
    14041 cacaacgcgg gccgcgccaa cttccaggtg gattggctgc cctgcgccgg agccccggtc
    14101 gaggacctcg tcgacctcgg ctggaccacg cccgacgagt tcgtcaccgg catggacgcg
    14161 ttcgcgacct tcgtcgtcct cagcctgcgc gtcgacgggc tcaccgcctt gcgcttcgtg
    14221 ccggtgacgg gcgaggggcc gggcggtttc ggtatgccgc acgacctcac tttccccggc
    14281 gagaccggca ccctcaccct gggctcgacg ccagacccgg cgtcgccgac ggtccaggtc
    14341 gcgcacacct ccatggccac gcctccggcg atctacgact acgacgtgag ggcccgcacc
    14401 ctgttgctgc tcaaggcgca cccggtgccg ggggtcgacc tcagcgcact tcgggagacc
    14461 cggatctggg ccaccgcgcc cgacggggtg gcggtgccgg tgtcgctcgt ccaccacgcc
    14521 gacgtgcgcc ccgacgggac ggcggccgtc ctgctctacg gctacggcgc ctatggcatc
    14581 ccgtcggacc cgtggttctc cgtcgcccgg ctctcgctgc tgcaccgcgg ggtcgtcttc
    14641 gcgatcgccc acgtccgggg tggcaccgag ttgggctggg actggtatgt ccagggccgg
    14701 ctggagcaca agccccacag cttcaccgac ttcctcgcct gcgccgacgc gctggtggac
    14761 cagggctggg cggcagccga tcgggttgct gcggagggag gctcggccgg tggactgttg
    14821 gtcgccgccg cagcgacgca ggcccccgcg cggttccggg ccgtgctcgc ccaggtgccc
    14881 ttcgtcgatg tcctcacgac gatgctcgac ccgagcctgc cgctgacggt caccgagcag
    14941 caggagtggg gcgacccgat cgccgacccc gcggcatacg acctcatcgc ctcctactcg
    15001 ccctacgaga ccgttccggt cggcgacttc ccggcgctgc tcatcacgac gagcgccaac
    15061 gacacccgcg tgttcgtcac cgaacccgcc aagtggatgg cgcggcttcg cgaccgggtg
    15121 accgacgatc cggtatgccg cccgctcctc atgcgcacgc aactcacctc gggtcacgga
    15181 gggcgttcgg ggcgctatca ggcctggcgt gagatcgcct gggagtgggc ggtgctgctc
    15241 gatctgttgg gcgccgcaga tcagagctga gtcggcccgg cgaaggtccc gcgggcggac
    15301 aactccaggg tgatcgccgt ggcaacgacg gcgacgagca gcagggccac gaggacgacg
    15361 agttggaccg cggccgcctg caggggcgtt gcgccgccga gcagcatccc gacgaaggcg
    15421 cccgggaggg tcaccaaccc gaccgttcgg gtctggtcca gaccggggac gagggcgagc
    15481 ccggcgtcgc tgcgagacac cagcagccgg gcgtcgcgat cgagcagccc caaggacagg
    15541 gccgcctcca cctcgccgtg ccgcgagcgc aacgcgtctg ccacccggcg gccggacagg
    15601 gtggtcgccg tcatggctcc gccgacgagg atgccgacca ccgggacgac ggcgatcggc
    15661 acgagaggca ccaccccggt cgcgagcagg atcgtcgcag tgggcagggc tccggcgcag
    15721 atgggcacga acccccagat ccagccgcgg gaccccgggg gtgcgacccg gcgcccggct
    15781 gtcaccgacg cgacggcgag catgagggca gcgaagcctg cggtcagcca ccaggagtcg
    15841 aggacggccg cgatgaccga cccgacgagg gccaactgga gcgtcgcccg cagggcagcg
    15901 gtcacctggg ctcgggagta gcccagcccg gccactcggc tcaccgcggc ggcgagcgcg
    15961 acgaggacgg ccaggacgag gcccagccgg acgagatcag ccgtcgtgtc gaccgaaacc
    16021 aggccctggg tggggatccg catacggcca tcatcgccgg tggccggtcg gctgtggacg
    16081 ggccaagggg aggtcggtgg acccgcctag actttgcccc gtgatgaatc ggaccgacct
    16141 gcgtggctca cgcctgtcgc ggcgcacctt gcgtgaggtg ctcccgaggg ccgagttcga
    16201 cgtggctgca gccgccgaag cggtgcgccc cgtcgtccag gacgtcgctc atcgtggggc
    16261 cgcggcgctg cgcgagttgt ctgagcggtt cgacggggtg gtcccgccgc agttgcgggt
    16321 ccccgccgac gtgctcgagg ccgcggtcgc ccaactcgac cccgccctgc gcgcggcact
    16381 cgaggagtcg atccggcgag cccggatcgt ccaccacgac cagcgccggg ccgacgtcac
    16441 cacccgcgtt gtcgaaggtg gcaccgtcac cgagcggtgg gtgccggtcg agcgggtcgg
    16501 cctctacgtc cccggtggcc tcgcggtcta tccctccagc gtcgtcatga acgtcgttcc
    16561 cgcgcaggcg gccggagtcg gctcgctcgc cgtcaccagc cctccgcagc gcgagaacgt
    16621 cggcgtgttc gccggctacc ccaaccccac gatcctcgcc acgtgcgcgt tgctaggggt
    16681 cgacgaggtg tatgccgccg gcggcgccca ggcgatcgcg atgctcgctt acggcttcga
    16741 cgagaccgtc acacacgccg atggcagcac cgagactctg gtctgcgagc cggtctccct
    16801 catcaccggg cccgggtcga tctgggtgac ggccgccaag cgtctcgtcc agggcctggt
    16861 cggcatcgac gccgaggccg gaccgaccga gattgccgtc cttgctgacg acagcgccga
    16921 ccccgagcac gtcgccgccg acctgctcag ccaggccgag cacgaccccc tcgccgcatc
    16981 cgtgctcgtc accgtcagcg aggcgctcgc cgatgccgtc gatgcggcgc tggcccggcg
    17041 ggtccctgac gccaagcacg gagagcgcat ccgtgaggcg ctcaccggac gtcagtcgcg
    17101 ggtcgtcctc gtcgacgacc tcgatgccgg gatcctcgtc tgcgatgtct atgccgccga
    17161 gcacctggag atccagaccc gcgacgccgc cgaggtggcc ggccggatcc acaatgccgg
    17221 cgcgatcttc gtcggcccct ggtcaccagt gagcctgggc gactatgccg ccgggtccaa
    17281 ccacgtcctg cccacggcgg ggtcggcggt ccactccagt gggctgtccg tgcagtcgtt
    17341 cctgcggggc atccacgtcg tcgcctacga cgaggcggcg ttgcgtgggg tcgcccacca
    17401 cgtcgtcacg ctcgcccagg ccgaggacct gcccggccac ggccaggccg tcacggcccg
    17461 gttcggcgac gcggtgccga cgccatgagc gcgccggcgg tgccgaccga cgtggcgcag
    17521 cgggtcgcca ggctccttcg cgacgacctg cgtggccaga cggcatacgg ggctccgcag
    17581 ttggacgtcc ccgtgcgcct gaacacc
//
LOCUS       DAOMZY010000021        17375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_2921_length_17375_cov_6.116109, whole genome shotgun sequence.
ACCESSION   DAOMZY010000021 DAOMZY010000000
VERSION     DAOMZY010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 17375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17375
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2921_length_17375_cov_6.116109"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="PLK64_02380"
     CDS             complement(<1..1054)
                     /locus_tag="PLK64_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3' terminal RNA ribose 2'-O-methyltransferase
                     Hen1"
                     /protein_id="HPV78617.1"
                     /translation="MFLTITSTAPDATGLGHLLHKHPERVQRFDVSVGAAMVFYPEAT
                     DERCTVALMVDVDPIGLVRRGRSGGEPFALAHYVNDRPYAASSMLAVAIGQVFRTALR
                     GECASRPELAASALPLKVHVPSAPCRGDADLVARLFEPLGWAVDAVSLPLDPALPHFG
                     DSRYVDLTLTGNVRLADALAHLYVLLPVLDGSKHYWVGDDEVDKVVRRGGAWLQGHPE
                     RDFVLRRYLAHRRSLVADATERLAALDDSLAFDATPSALPDVDSIDSDSAGNSFSAGI
                     VELPPVRLAEHRIAAVLAALQEIKAAHVVDLGCGSGSLLARLLGDAAYARVVGVDVSA
                     RELAWAARRLHVDRMSE"
     gene            1212..2330
                     /gene="nagA"
                     /locus_tag="PLK64_02385"
     CDS             1212..2330
                     /gene="nagA"
                     /locus_tag="PLK64_02385"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012750816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HPV78618.1"
                     /translation="MDTLLAADRIFTPEEILEPGWVEVAGTTVKAIGSGHHPHREPDW
                     HTTTLAPGLVDTHVHGGGGASFSTTSGDEAALAAMAHQRAGSTSVIASLVSAPIPVLA
                     DQLACLADLCDDGILAGLHLEGPWLSPTHRGAHDLRHLARGNAAAVDRLVEAARGHLR
                     MATVAPEILGGFEVIKALVDADVLVAVGHTAADTATTEEAVEAGARIATHLFNGMPPL
                     HHRFPGPVGVLLANPSVTVELIADGVHLAPATLALAARAAAGRVSLVSDAMSGCGQPD
                     GRYVLGSTPVTVVDGVARVTSSGALAGGTRTLAHGIRTLYAAGVPLADAITAATATPA
                     RALGLWRRGRLVVGANADLVALDDDLAVTRVMVNGTWR"
     gene            complement(2361..4376)
                     /locus_tag="PLK64_02390"
     CDS             complement(2361..4376)
                     /locus_tag="PLK64_02390"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HPV78619.1"
                     /translation="MTAYPHLLAPLDLGFVTLPNRALMGSMHVGLEEVPGGFERMGAF
                     YAERARGGAALIVTGGFGPNEAGALLPGAALLTTPEEADRHRVVTDAVHAAGGRICLQ
                     ILHAGRYAAHLGAVAPSPIQAPISPITPRELTTAEVGETVADFARCAALAQAAGYDGV
                     EIMGSEGYLINQFLVTRTNQRTDEWGGSYAARMRFPVEVVRAVRETVGPNFIVVYRLS
                     MIDLVEDGSTLDEVVELARGVEAAGATMLNTGIGWHEARIPTIATSVPRAGFAWVSGK
                     VKAAVGIPVVATNRINTPDVAEQVLADGLADMVSMARPFLADPLFVTKAAAGQADRIN
                     TCIACNQACLDHTFAGKLTSCLVNPQACHETELIIAPTDSPKRIAVVGGGMAGLACAT
                     TAARRGHAVTMFEADSDIGGQFNLARQIPGKEEFSETLRYFRGELVATGVDVRLGTRA
                     GEGELAAYDTAILATGVTPRIPDVEGVDHPTVVSYLQVLRDHVPVGDTVVIMGAGGIG
                     FDVAEYLTQSGPSGALDPTAFNAEWGIDTTYAHRGGVVAAQPEPPARKVFLVQRKDSK
                     VGAGLGRTTGWIHRAQLAARGVAFVAGARYDRIDDAGLHITIGERAAVLPADTIVLAT
                     GQEPNRELYAALEASGVDVRLIGGADVASELDAKRAIKQGTEVAAAL"
     gene            complement(4451..5155)
                     /locus_tag="PLK64_02395"
     CDS             complement(4451..5155)
                     /locus_tag="PLK64_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPV78620.1"
                     /translation="MPPSPRTRPPLHRVTVLDAAVELADAEGIEAVSMRRLAERLGVV
                     PMALYKHVSDKEDLLDGMIDRVIAQFQPGDPSADPSADPSAWPDAVRRVLLSTREVVQ
                     RHPWARRVIETRTHRTPAVLAYMEAVTQLFLGGGLSPDLTHHVMHVLGNRIWGFSPEL
                     FNDPGGRPASDRPRTSGQPVPSDFPGILAIAADASARRPGATGCDEEFEFRFALDLLL
                     DGIARLHASEWASPPL"
     gene            5257..5988
                     /locus_tag="PLK64_02400"
     CDS             5257..5988
                     /locus_tag="PLK64_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPV78621.1"
                     /translation="MARVLVVDDEPQIRAILRAYLSAEGFQVQEAATGAQALHLLATE
                     PPDLVLLDIGLPDLDGLEVLRTLRKTSDTYVVLVTARAEEVDKLIGLGVGADDYVTKP
                     FSPREVVARVKAVLRRTRPLGPAATDPGDTDVLHFDSVSVDTGRREVRVGGIPAILTA
                     LEFDLLVALAESPGRVFSRAQLLEKVWGYDFFGDERVVDVHIRGMRKALGDDATAPRL
                     IGTVRSVGYKFLLDPIDRLTTEGAS"
     gene            5985..7202
                     /locus_tag="PLK64_02405"
     CDS             5985..7202
                     /locus_tag="PLK64_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPV78622.1"
                     /translation="MNRLVVRLVISHLLVAVVAALATFLVVRQLAPALFDASLRVGQG
                     GGLGQGSGAQGTAGGAGMVLRQQFGDAVDRALAVGAVVGALAAALVGALAAYRLIRPL
                     DALRAATRAMAQGTYAVPLPTPRTTELAELTDDVKLLGQQLGETETQRVRLLGEVAHE
                     MRTPLTVIDGTVEAMIDGILPLEPDQLAVVSDEVRRLRRLSDDLSALSRTDEGRLSLV
                     LADVDLREVVSRAAGRLRAQAEDAGLDFEIDCGDANVPVRVDAERIGQVVTNLVGNAV
                     RATPEGGRVRVRCGPSESAGRHGAASAVIIVEDTGEGLAGADLTRIFERFYRVPGRRA
                     PEHETGSGIGLTIARGIVAAHGGQLTATSAGPGQGATFTVTLPLARPGESHDRSDVTG
                     LASAEVHNPGGRR"
     gene            7344..8051
                     /locus_tag="PLK64_02410"
     CDS             7344..8051
                     /locus_tag="PLK64_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPV78623.1"
                     /translation="MPIPQGGLPSTRGLLRDEVYARLCDAIVDGTFRPGEQLRDNEIA
                     HWLGVSRTPVREALLRLARIGLVISTPGKSTLVADIEPQTVRDAQEVVTALHELAVRT
                     AIPFIRPGDIETMRRANRRFEAALAAGDADAAIRADDDLHGVAVAVTANLAICSVLEQ
                     YVPVLRRAERLRFSSLSGRRSVTWHERLISAMEVGDADAAAAASTAAWSTLARLIDLE
                     RAIDHANGATDADRPTR"
     gene            8061..9419
                     /locus_tag="PLK64_02415"
     CDS             8061..9419
                     /locus_tag="PLK64_02415"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPV78624.1"
                     /translation="MRSRRRRIVLRVLGGLLALVLVALVGGYLYAKPLLLTGTGYAAH
                     NDCAVRAIAGRSDPRADLPPNPLVPYLRSSQAADNSSYSATVLGVLAGQTAYYGDAGC
                     TLGTPLTGTGPLTQVPVGANPFDAVAVPAPSSAVAAAVAKAFGDDLTPFEQDLLGTRA
                     VVVVQGGKLVAERYAPGFTAATPQLGWSMAKSAASLLTGRVVKEGKVAVTDAGLRPEW
                     TDDRSKITVDQLMRMTSGLTWNEDYDLGTAITAMLYLEPDMAKFVASQPLAHAPGTYQ
                     QYSSGSTNLLCSVLRQRTGLGVDLPRRELFAPLGLSSAVWEPDGSGLPVCSSYLWATP
                     RDWAALGQFALQDGVWGGQRLLPEGWMAATTRSDAVATTEDPGYAAGWWANRLADGTL
                     DQPTLPADTYRANGHDGQRIYVVPSADLVVVRLGFSPSIKTPEQLRVVDLVATLAGMP
                     RA"
     gene            9569..9994
                     /locus_tag="PLK64_02420"
     CDS             9569..9994
                     /locus_tag="PLK64_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HPV78625.1"
                     /translation="MTQPVRDRKTQPTQGDVDAHLASITHEGRRADAQAAVELFREVT
                     GAEPQMWGTSMIGFGRMIYTGSDHKMRETMAVGLAARAAALTLYGLTFYGSNEALLAR
                     LGPHTTGKGCLYLKRLSDVDVDVLRELIARGWTENHQPY"
     gene            complement(10026..10358)
                     /locus_tag="PLK64_02425"
     CDS             complement(10026..10358)
                     /locus_tag="PLK64_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HPV78626.1"
                     /translation="MAFDPELADRLREVLADEPGLREQRMFGGLGFMLDGHMAVAASS
                     DGGILLRIDPAAAGDLVDGVVARRFEMRGRAMDGWLAVDQAAAHTDEALREWVAHGVA
                     YVRSLPPK"
     gene            complement(10361..10933)
                     /locus_tag="PLK64_02430"
     CDS             complement(10361..10933)
                     /locus_tag="PLK64_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HPV78627.1"
                     /translation="MAEILLFHHIQGLTHGVIAFADALRAGGHTVHTPDLFDGRTFDS
                     IEAGQAYLGEVGFDEVRERGILAAEGLPAGLVYAGFSLGVMPAQQLTQNRPGARGALL
                     YHSFADPSYFGTWPVGVPVQIHSMDADPFFVGEGDIEPAEAFVAAHDDAELFLYPGGG
                     HLFADSSLAEYDAVATEIVIERSLALLARV"
     gene            complement(10926..11525)
                     /locus_tag="PLK64_02435"
     CDS             complement(10926..11525)
                     /locus_tag="PLK64_02435"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPV78628.1"
                     /translation="MTTQPGRARPTDADGFRAAWALAEAEWAQTFARARSFSAQQLHE
                     RVDGEWSFIQTQRHLVFATDAWVRRALLGDPTPWHPLDLPHDDMEDAPGVPRDYAARP
                     TLDEVLALRADRMATVRDVIAALTDEALDSLTVPVLEPGYPESEAFLVRRCLGAVVNE
                     EWEHRRFAERDLDIIDNGSRSTDQTTETDQPREEETTHG"
     gene            complement(11536..12219)
                     /locus_tag="PLK64_02440"
     CDS             complement(11536..12219)
                     /locus_tag="PLK64_02440"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV78629.1"
                     /translation="MTDLAATVEGIGALAEPVRRALYDFVVASRGPVGREEAAEAVGV
                     PVHTAKFHLDRLADDGLLDVEFQRRTGRTGPGAGRPAKLYRRSTQEFAVSLPPRHYDL
                     AGRILADAVEQAAAGVPLEGALRAAAERAAYRVVESVPTDGDRLPQVLAGLGYEPREE
                     EAGLRLVNCPFHSMAAEHTALVCGVNRDFVQGVLDALGRGDVQARLTPTPGQCCVTVV
                     PTTDVGARA"
     gene            12311..12973
                     /gene="folE"
                     /locus_tag="PLK64_02445"
     CDS             12311..12973
                     /gene="folE"
                     /locus_tag="PLK64_02445"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HPV78630.1"
                     /translation="MSQAATPTVPAGEAPTAYPLLGPRRPRVTGHAPLVDLGRAEAAA
                     TELLRALGLPIDTEAMCDTPGRLTRAYLELLSVPDFEMTTFANDEGYDELVVVADIPV
                     RSLCEHHMLPFVGVAHVGYVPGDRLLGLSKFARTVDFFARRPQTQERLTSQIADHLDA
                     HLSPQGVGVVISAEHTCMTLRGARAAGTRTRTSATRGVLRNHLATRAEFLSRAVTLED
                     SR"
     gene            12970..14100
                     /locus_tag="PLK64_02450"
     CDS             12970..14100
                     /locus_tag="PLK64_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPV78631.1"
                     /translation="MNPIVVVGAGLAGATAVTALREGGHDGPLVLLGAESHPPYERPP
                     LSKGYLLGNDPIESAFVHDRQWYAAHDIDLRTGAFVTDLDLGRKSVSLLDGTEIAYDQ
                     LLLATGSRPRRLPLADDSGVPTAYLRTIEDSDRIRAAFGPGHRIVIIGAGWIGLEVAA
                     AARAAGTEVTVFEVAELPLLRVLGPRVAQAFADLHRAHGVDLRLGTAVSAADLAGATL
                     VVVGVGVAPDASLAQGAGLAVSNGVLVDATLRTSHPDVYAVGDIANHDHPLLGRRVRV
                     EHWDTAIEQAKVAAHNLVGGQEAEAYDRLPHFFTDQYDLGMEYVGYAAADADTEVVVR
                     GDLGAEQWRAYWVSGGVVVAGMHVNEWGAIDEIREQIGHPLA"
     gene            complement(14305..15639)
                     /locus_tag="PLK64_02455"
     CDS             complement(14305..15639)
                     /locus_tag="PLK64_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HPV78632.1"
                     /translation="MEDSRGAVSLTDRVTTLQAARDVLSGLAEVLWQAQGAELAGVLT
                     LLDEVAGLAGAGRVAVTREAFTRGEVAASQAGSLTGWVAEHAPGLAVAGGGGQVAAVV
                     EAGLRRATRPVTDAACSGRVPVPVAVAVIREAERLKPQLQPEAVPTVVEAMLTMGAGH
                     GVRAVRELRARLIADYGWPGQLQADQDAAAALVSLSAPVGDELGAFSYRLVLDAEGKA
                     VLEAAIGPLSKPAPDADGAPDRRPARQRRGQALIEVCRRATAAGARPPVGVKTTLVVT
                     MGLAELTERLSAGVAVGSSESGTVIAPETVRRLACDAQVIPVVLGGPSEVLDWGRTAR
                     LFTPGQVKALWLRDQSCTFPGCGIPAHWCDAHHVTHWVDGGRSDLSNAALLCGRHHTV
                     AHRDRLTAAVTAAGVAWDTAHGSYDRALNATAQPRGRPPDPGLARGPTEPAA"
     gene            15788..16918
                     /locus_tag="PLK64_02460"
     CDS             15788..16918
                     /locus_tag="PLK64_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HPV78633.1"
                     /translation="MRLSQQDARRIAVQAQLLTADRPANLFEVLDRLTLLQLEPTKVV
                     APTHHLVLWSRLGRSYSRDELADALAAREVIEVQLHLRPAWFVALLRAEMAAWPGGGA
                     GAGAGASSPPEWQIRIAEWLKTNDACRRDILHRLDAEGAQPAGALPDTCVVPWRSTGW
                     TNDKNVQRMLDFMEQRGEIAVSHRAHGQRHWDLAERVYPDDPPVPAAEARRERDALRL
                     HALGIARARATARQVEPDDIGEAGEPAVIEGVRGRWRVDAAYLDGLGDDTFRPRVALL
                     SPLDRLVFDRKRMADLFDFDYKLEMYQPKATRRWGYWALPILVGDRLVGKVDATADRE
                     AGVLRVDAVHEDEPFDEPTATQIRAELASLAGWLGLDLDERF"
     gene            16992..>17375
                     /locus_tag="PLK64_02465"
     CDS             16992..>17375
                     /locus_tag="PLK64_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78634.1"
                     /translation="MSRLRSPIPWLAALLTLALSLFVSVPAFAAPPSLAGADGTGERT
                     WSPRVCAFTDDEIKAEEETIKTEQGTIILNDHILCALANESQPKNPVEQAIIDAFLAR
                     TGFDLDAAREHHLGAVLCSDGGVGMV"
BASE COUNT         2728 a         6258 c         5866 g         2523 t
ORIGIN      
        1 gctcagacat tcgatcgacg tgcaatctcc ttgctgccca ggcaagctcg cgcgcggaca
       61 catcgacacc aaccacgcgc gcgtatgccg cgtcgccgag cagccgcgcg agaaggctcc
      121 cgctcccgca gccaagatcc acgacgtgcg ctgccttgat ctcctgaagg gcagccagca
      181 ccgcagcgat ccggtgttcg gcgaggcgca cgggcggcaa ctccacaatg cccgcactga
      241 atgagttgcc cgcactgtct gagtcgattg aatcgacgtc cggcagcgcc gagggcgtcg
      301 cgtcgaacgc cagcgaatcg tccagcgcag caaggcgttc ggtcgcatcc gccaccaagg
      361 agcgccggtg agctagatag cgacgcagca cgaagtcacg ctcgggatgc ccctgcagcc
      421 aggcacctcc tcggcgaacc actttgtcca cctcgtcgtc accgacccag tagtgcttcg
      481 agccgtcgag gaccggaagg aggacgtaga ggtgagcgag agcgtcggcc agacgcacgt
      541 ttcccgtcag cgtgagatcg acatagcgcg agtcgccgaa gtgcggcagc gccgggtcca
      601 acggcaggct aacggcgtcg accgcccagc ccaacggctc gaacaagcgc gcaacgaggt
      661 cggcgtcgcc gcggcacggc gctgacggga catggacctt gagtggcagc gcactggcag
      721 cgagctccgg gcgggacgcg cactcacccc gcagagcggt ccggaatacc tggccgatgg
      781 ctacggcgag catcgaggat gccgcgtagg gccggtcatt gacgtagtga gcgagcgcga
      841 aaggctcccc gccggaccgt ccgcggcgca ccagaccgat cggatcaacg tcaaccatga
      901 gcgcgacggt gcaccgctcg tcggtcgcct ctggatagaa gaccattgcg gctcccacgg
      961 acacgtcgaa ccgctgcacc cgctcaggat gcttgtgcag caggtggccc aggccggtgg
     1021 catccggggc tgttgacgtg atggtgagga acaccgtttc atcttccccg gactgcccct
     1081 cccacgcaca gcgggtttcc gggcgttttc ttgggcgagc cgagggctgt gagcgcgaga
     1141 caatgggcca gtcgcttcga ccgcgacgaa cggcgagtga ggaacgacga acggaagggg
     1201 tgcgaggcgt catggacaca ttgctggccg ccgaccgcat cttcactccc gaggagattc
     1261 tcgagccggg ctgggtcgag gtggctggca caaccgtcaa agccatcgga tcgggccacc
     1321 acccgcaccg tgaacccgat tggcacacaa cgactctcgc ccccggcctg gtggacacgc
     1381 acgtccacgg cggcggcggg gcctcgttct cgacgacgtc cggcgacgag gcggcgttgg
     1441 ccgcgatggc ccatcagcgg gctggcagca cgagcgtcat cgccagcctc gtctccgctc
     1501 ccattccggt gctggccgac caactggcct gtctggccga cctgtgcgac gacggcatcc
     1561 tcgccggtct gcacctcgaa ggcccctggc tcagccccac ccatcgcggc gcccacgacc
     1621 tgcgccacct cgctcgcggc aacgcggcgg ccgtcgaccg gctcgtcgag gcggcccgcg
     1681 ggcatctgcg gatggcgacg gttgcccccg agatcctcgg tggcttcgag gtcatcaagg
     1741 cgcttgtcga cgcggatgtc cttgtcgccg taggccatac ggccgccgac acggcaacta
     1801 ccgaggaggc tgtcgaggcg ggtgcccgga tcgccaccca cctgttcaac ggtatgccgc
     1861 cgctgcacca tcgcttcccc ggcccggtgg gcgtcctcct cgccaaccca tcggtcaccg
     1921 tcgagctcat cgccgacgga gtgcacctgg cgcccgcgac cctggccctc gccgcccgcg
     1981 cagcagccgg acgggtgagc ctggtcagcg atgcgatgtc cggctgcggc cagcccgacg
     2041 ggcgctacgt cctcggctcg acgccggtga cggtcgtcga cggggtagcc cgcgtgacgt
     2101 cctccggtgc cttggcaggc ggcacgcgga ctctcgcgca cggcatacgc accctgtatg
     2161 ccgcgggcgt tcccctcgcg gacgccatca ccgctgccac ggcgacccct gcgcgagcgc
     2221 tcgggctgtg gcgccgtggc cgactcgtcg tcggcgccaa cgccgatctc gtggccctcg
     2281 atgacgacct cgctgtcact cgggtcatgg tcaacgggac ctggcgctga ccgctcgacg
     2341 acgagccccg gcagcatcgg ctacaaagcc gcagcaacct ccgtgccctg tttgatcgcc
     2401 cgcttggcat cgagttcact ggcgacgtcg gccccaccga tgagccgcac gtcgacaccg
     2461 gaagcctcga gggcggcata caactcgcgg ttgggctcct gcccggtggc cagaacgatg
     2521 gtgtcggccg gaaggacggc ggcgcgttca ccgatggtga tgtgcaggcc cgcatcgtcg
     2581 atccggtcat accgagcgcc ggcgacgaag gcgaccccgc gggcggccag ctgtgcgcgg
     2641 tggatccagc cggtcgtgcg cccgaggccc gcaccgacct tggagtcctt gcgctgcacg
     2701 aggaacacct tgcgcgccgg cggttccggt tgcgccgcaa cgacgccgcc gcgatgggcg
     2761 taggtggtgt cgatccccca ctcggcgttg aacgccgtcg ggtccagggc gccgctcggg
     2821 cccgactgag tgagatattc ggcgacgtcg aagccgatgc cgcccgcgcc catgatgacg
     2881 accgtgtcac cgacggggac gtggtcgcgc agcacctgca ggtaggacac gaccgtgggg
     2941 tggtcgacgc cctcgacgtc ggggatgcgc ggagtcacgc ccgtggccag gatggcggtg
     3001 tcgtatgccg ccagctcacc ctccccggca cgggtcccga gccgcacgtc gaccccggtg
     3061 gccaccaact cgccccggaa gtagcgcaag gtctcgctga actcctcctt gccggggatc
     3121 tggcgcgcca ggttgaactg cccgccgata tcggagtcgg cctcgaacat cgtcaccgcg
     3181 tggccacgac gcgccgccgt tgtcgcacaa gccaatccgg ccatgccgcc cccgacgacg
     3241 gcaatccgct tcggtgaatc ggtgggggcg atgatcaact cggtctcgtg gcaggcttgc
     3301 gggttaacca ggcaggacgt gagcttgccg gcgaacgtgt ggtccaggca ggcctggttg
     3361 caggcgatgc aggtgttgat ccgatctgcc tgtcccgcag ccgctttggt gacgaacagg
     3421 ggatcggcga ggaacgggcg ggccatcgac accatgtcgg cgagcccgtc ggccaacacc
     3481 tgctcggcca cgtcaggagt gttgatccgg ttggtcgcaa cgaccggaat gcctactgcc
     3541 gctttgactt tgccgctcac ccacgcgaac ccagcgcgcg gaacgctggt ggcaatcgtc
     3601 gggattcggg cctcgtgcca gccgattccc gtgttgagca tggtcgcgcc cgccgcctcg
     3661 acgccccgcg cgagctcgac gacctcatcg agcgtcgagc cgtcctcgac caggtcgatc
     3721 atcgaaagcc gatagacgac aatgaaattc gggccaaccg tctcgcgaac cgcccggacg
     3781 acttcgaccg ggaagcgcat ccgcgcggca tacgacccgc cccactcgtc cgtgcgctgg
     3841 ttggtgcggg tgacgaggaa ctggttgatg aggtagccct ccgaccccat gatctcgacc
     3901 ccgtcgtagc ccgccgcctg cgcgagcgca gcgcagcgcg cgaagtcggc cacggtctcc
     3961 cccacctcgg cggtcgtcaa ctcgcgcggg gtgatcggcg agatcggggc ctggatgggg
     4021 ctcggcgcga cggcgccgag gtgcgcggca taccgaccgg catgcaggat ttgcaggcag
     4081 attcggccgc ccgcggcgtg aaccgcgtcg gtgacgaccc ggtggcgatc cgcttcctcg
     4141 ggggtcgtca gtagggccgc acccggcagc agggcgcccg cctcgttggg cccaaagccg
     4201 ccggtgacga tgagcgcggc gccgccgcga gcgcgctcgg catagaaggc ccccatccgc
     4261 tcgaagccgc cgggcacctc ctccagcccc acgtgcatcg agcccatgag cgctcggttg
     4321 ggaagggtga cgaagccgag atcgagcggg gccagcaagt gggggtatgc cgtcacggcg
     4381 gtctccgtcg cttcgcctgg gacgtccctc ggtcggcacg cccgcgtgtg gcctcgatgt
     4441 tactcgtcag tcacaacggc ggggaggccc actcggaggc atgaagtcgc gcgatcccgt
     4501 ccaggagcag gtcgagcgcg aagcggaact cgaactcctc atcgcagccg gtcgcgccgg
     4561 ggcgccgcgc cgaggcatcc gccgcgatcg cgaggatgcc ggggaagtcg ctcggaacgg
     4621 gctggccgct cgttcgcggc cgatccgacg ccggacggcc gccggggtcg ttgaacaact
     4681 ccgggctgaa gccccagatc cggttgccga gcacgtgcat cacatggtgg gtgaggtcag
     4741 gcgagagccc gccgccgagg aacaactggg tgacagcctc catataggcg agcaccgcgg
     4801 gggtgcggtg ggtgcgggtc tcgatgaccc gccgggccca ggggtggcgt tggacgacct
     4861 cgcgcgtcga cagcagcacc cggcgaaccg catcgggcca cgcgctgggg tccgcgctcg
     4921 ggtccgcgct gggatcgcca ggctggaact gggcgatgac ccggtcgatc atcccgtcaa
     4981 ggagatcctc cttgtcggac acgtgcttat agagagccat cgggacgacg cccagccgtt
     5041 cggcaagcct gcgcatgctc accgcctcga tgccctcggc atcggccaac tcgacggccg
     5101 catcaagcac cgtcacccgg tgcagcgggg ggcgggtccg gggggagggc ggcatacggg
     5161 ctcctgggca gggggctgac gggtgtacat cgtacgcttt cgcgcagatg cggccggtgt
     5221 ctctagcctg ggagcagaca gaaggaggtg caacggatgg ccagggtcct ggtcgtggac
     5281 gacgaacctc agatccgcgc catcttgcgg gcctacctga gcgccgaagg gttccaggtc
     5341 caggaggcgg cgaccggcgc ccaggcgttg cacctactcg ccaccgagcc tcccgacctc
     5401 gtcctgctcg acatcgggct gcccgacctc gacggcctcg aggtcctgcg gaccctgcgc
     5461 aagacctccg acacctacgt cgtcctcgtc accgcccggg cggaagaggt cgacaagttg
     5521 atcgggttgg gggtcggcgc cgatgactac gtcaccaaac ctttcagccc gcgcgaggtc
     5581 gtcgcgcgcg tcaaagctgt gctccggcgc acccggccgc taggcccggc ggcgaccgat
     5641 cccggcgaca ccgacgtgct ccacttcgac agcgtcagcg tcgacaccgg tcgcagggag
     5701 gtgcgggtgg gcggcatacc ggccatcctc acggccttgg agttcgacct gctggtggcg
     5761 ctcgccgagt ctccagggag ggtcttctcg cgggcccaac tgctggaaaa ggtctggggc
     5821 tacgacttct tcggcgacga acgggtcgtc gacgtccaca tccgcgggat gcgcaaggcc
     5881 ctgggtgacg acgcgaccgc gccgcgcctc atcgggaccg tccgcagcgt cggctacaag
     5941 ttcctcctcg acccgatcga ccgcctcaca acggagggcg cgtcatgaac cggctcgtcg
     6001 tgcgactggt gatctcgcac ctgctcgtcg ccgtggttgc ggccctggcc actttcctcg
     6061 tcgtgcgcca actcgcgccg gccctcttcg atgccagcct gcgggtcggc cagggcggtg
     6121 ggctggggca aggatccggc gcccagggga ccgccggagg cgccggaatg gtgctgcgcc
     6181 aacagttcgg cgacgccgtc gaccgggcct tggccgtcgg agccgtggtc ggcgcactgg
     6241 cggctgccct cgtgggggcg ctcgcggcat accgactcat ccggccgctc gacgccctcc
     6301 gcgcggcgac gcgggccatg gcgcagggga cgtatgccgt cccgctcccc acaccgcgca
     6361 cgaccgagct cgccgaactc accgacgacg tcaagctcct ggggcagcaa ctcggcgaga
     6421 ccgagaccca gcgggtccgg ctgctcggtg aggtggccca tgagatgcgg accccgctga
     6481 ccgtcatcga cgggacggtg gaggcgatga tcgacggcat cctgcctctg gaacccgacc
     6541 agttggccgt ggtgagcgat gaggtgcggc ggctacggcg gctgtccgac gacctgtcgg
     6601 cgctgtcgcg caccgacgag ggccggctgt ccctcgtcct ggccgacgtc gacctgcggg
     6661 aggtcgtgag tcgggcggcc ggacggctgc gggcgcaggc cgaggacgcg gggctggact
     6721 tcgagatcga ttgcggcgac gcgaatgtcc ccgtgcgagt ggacgccgag cggatcggcc
     6781 aggtcgtcac gaacctggtc ggcaatgccg tccgtgccac cccggaaggt ggccgggtgc
     6841 gggtgcggtg cgggccgtcc gagagcgccg gacgacacgg cgcggcgtcg gccgtgatca
     6901 tcgtcgagga caccggcgag gggctggcag gcgctgacct cacgcggatt ttcgagcgct
     6961 tctaccgggt gccggggcgc cgagcccccg aacacgaaac cggatccgga atcggcctga
     7021 ccatcgccag aggcatcgtg gctgcgcacg gagggcagct cacagccacg tccgccggcc
     7081 ccgggcaggg ggcgaccttc acggtgacgc tgccactcgc gcggcccggg gagagtcatg
     7141 accgctccga cgtcaccggc cttgcgtcag ccgaggtcca caacccgggt ggcaggcgct
     7201 gactcggcca aacccccggt gactcctccc agtcatagcc cacgatctgt gacttcggac
     7261 acgaaaggac aacataagtc ttcagacgcc cggaaagtcc gaagcctcct acgggaggca
     7321 gggttgcgtg tatcaataga cccatgccca ttccccaggg cgggttgccg tccacccgcg
     7381 gactgctgcg cgacgaggtc tacgcgcggc tgtgcgatgc gattgtggac gggacgttcc
     7441 ggccgggcga acagttgcgc gacaacgaga ttgcccattg gctcggagtg agccggactc
     7501 cggtgcggga agccctgctg cggctggctc gcatcggcct ggtgatttcg acgcctggca
     7561 agtcgacgct cgttgccgac atcgagccgc agacggtccg cgacgcccaa gaggtcgtga
     7621 ccgcgctgca cgagttggcg gtccgcacgg cgatcccctt catccggccc ggagatatcg
     7681 aaactatgcg tcgggctaac cgccgcttcg aagcggcgtt ggcagccggt gacgccgacg
     7741 cggcgatccg cgccgacgac gacctgcacg gcgtcgcggt cgccgtcacg gccaacctcg
     7801 cgatctgttc cgtgctcgag cagtatgtgc ccgttctgcg acgggccgag cgactgcgct
     7861 tctcatcgct ctcgggtcga cgatcagtga cctggcacga acggctcatc agcgccatgg
     7921 aggtcggcga cgccgacgcc gccgccgcgg cgtcgaccgc ggcgtggtcg acgctggcgc
     7981 gcctcatcga tctcgagcgg gcgatcgacc acgcgaacgg cgcgaccgac gccgaccgac
     8041 ccacgcggta gcgtccgctc atgcgttcac gtcgccgccg gatcgtgctt cgggtgctgg
     8101 gtgggctgct cgccctcgtc ctcgtcgcgc tcgtgggggg ctacctctac gccaagccgc
     8161 tactgctcac cggcacgggg tatgccgccc acaacgactg cgccgtgcgc gccatcgcgg
     8221 gtcgttccga cccgagggcc gacctgccgc cgaacccgct cgtgccgtat ctgcgcagtt
     8281 cgcaggctgc ggacaacagc tcctactccg ccaccgtcct cggggtgttg gccggccaga
     8341 cggcctacta cggcgatgcg ggctgcacgc tggggacccc gctcacggga accgggccgc
     8401 tgacccaggt gcccgtcggg gcgaacccgt tcgacgccgt tgcggttccc gcgccgtcct
     8461 cggccgtcgc tgcagccgtt gcgaaagcct tcggcgacga cctcacgccg tttgaacagg
     8521 accttctggg gacgcgagcc gtcgtcgtcg tgcagggtgg caagctcgtg gccgagcgct
     8581 atgcccccgg attcaccgct gccacaccgc agttggggtg gtcgatggcc aagagcgccg
     8641 cgagcctgct caccgggcgg gtcgtcaaag agggcaaggt cgccgtcacc gacgccgggc
     8701 tgcgtccgga gtggaccgat gaccgctcca agatcaccgt cgaccaactc atgcggatga
     8761 cgagcgggct gacgtggaac gaggactacg acctcggcac ggcgatcacc gcgatgctct
     8821 atctcgagcc cgacatggcg aaattcgttg cgagccaacc acttgcccat gcccccggca
     8881 cctaccagca gtactccagc ggctcgacca acctgctctg ctcggtgctg cgccaacgca
     8941 ccggattggg cgtcgatctg ccgcgccgcg agctgtttgc ccccttgggt ctctcgtccg
     9001 ccgtgtggga gccggacggc tccgggctgc cggtgtgcag ttcctacctc tgggcgacgc
     9061 cgcgcgactg ggcggcgctc ggccagttcg cgttgcagga cggcgtgtgg ggcggacagc
     9121 gactgctccc ggagggctgg atggccgcga cgacccgctc cgacgccgtc gccacgaccg
     9181 aggaccccgg atatgccgcc ggctggtggg ccaaccgcct tgcggacggc acgctcgatc
     9241 agccgacctt gcccgccgac acctatcggg cgaacggcca tgacggacaa cggatctacg
     9301 tcgtcccgtc ggccgacctc gtggtcgtcc ggctggggtt cagcccctcg atcaagacac
     9361 cggagcagct gcgggtcgtc gacttggtcg ccactctcgc cggtatgccg cgcgcctgac
     9421 ccaacgcccc gccacggcga gtcagggcga gttcatcggg caggcgttgt tccagtgacg
     9481 acacgttcga caagacgagc gacgacacag gtggcgacac gagtgacgtc gcgcagggcg
     9541 aggcgcgggg aggcagcaca ctaggccgat gacccagccc gtgcgcgacc gtaagaccca
     9601 gccgacccag ggggacgtcg acgcccatct cgcgtcgatc acccatgagg ggcggcgagc
     9661 ggacgcccag gctgcggtcg agttgttccg cgaggtcacc ggcgccgagc cgcagatgtg
     9721 gggaacctcg atgatcggtt tcggccggat gatctacacc ggttcggacc acaagatgcg
     9781 cgagacgatg gcggtcgggc tggccgcgcg ggcggctgcg ctcacgctct atggcctgac
     9841 cttctacggt tcgaacgagg cgctgttggc ccggctcgga cctcacacca ccggcaaggg
     9901 atgcctctat ctcaagcggt tgtccgatgt cgacgtcgac gtcctgagag aactcatcgc
     9961 ccgcggctgg acggagaacc accagcccta ctgacgtcgc cgagccgcgg cgggccgggc
    10021 aagcgctact tcggcggcaa cgagcggacg taggcgaccc cgtgagcgac ccactctcgc
    10081 aacgcctcat cggtatgggc ggctgcctgg tcgacggcca gccagccgtc catcgcccgc
    10141 ccacgcatct cgaaccgccg cgccacaact ccgtcgacga ggtcaccggc cgccgcggga
    10201 tcgatccgca ggagtatgcc gccatcgcta ctggccgcga cggccatgtg cccgtcaagc
    10261 atgaagccca gcccgccgaa catccgttgc tcgcgcagcc caggctcgtc tgccagcacc
    10321 tcacgcagcc ggtcggccaa ctcggggtcg aaggccaccg tcagaccctg gccaggagtg
    10381 ccaacgagcg ctcgatgacg atctctgtgg ccacggcgtc gtactcggcc agcgaactgt
    10441 ccgcaaagag atgcccccca ccggggtaga ggaacaattc ggcgtcgtcg tgggccgcca
    10501 cgaaggcctc ggccggctcg atgtcgccct caccgacgaa gaaggggtcg gcatccatcg
    10561 aatggatctg cacggggaca ccgacgggcc aggtgccgaa gtaggacggg tcggcgaacg
    10621 agtgatagag cagcgcgccg cgggcacccg gccggttctg agtcaactgc tgggccggca
    10681 tcacgccgag ggagaacccg gcatacacca ggcccgcagg gagcccctcc gcggcgagga
    10741 tgccgcgctc ccgcacctcg tcgaagccca cctcgccgag ataggcctgc ccggcctcga
    10801 tcgagtcgaa ggtccgcccg tcgaacaggt ccggcgtgtg caccgtgtgc ccacccgccc
    10861 gcagtgcgtc ggcgaacgcg atcaccccgt gcgtcagccc ttggatgtgg tggaacaaca
    10921 ggatctcagc catgcgtggt ctcctcttcc cgaggctggt cggtttccgt ggtctggtcg
    10981 gtgctccgcg aaccgttgtc gatgatgtcc aggtcgcgct cggcgaaccg acgatgctcc
    11041 cactcctcgt tgacgaccgc ccccaggcat cgacgcacca ggaacgcttc ggactcggga
    11101 tatcccggct caagcacggg caccgtcagc gagtcgaggg cctcgtcggt cagcgctgcg
    11161 atcacgtcgc gcaccgtcgc catccggtcg gcccgcaggg ccagcacctc atcaagcgtg
    11221 ggacgagcgg cgtagtcgcg cgggacaccc ggggcgtcct ccatgtcgtc gtgcggcagg
    11281 tccaacgggt gccacggcgt cgggtcaccg agcagcgccc ggcgcaccca agcatcggtc
    11341 gcaaagacga ggtgccgttg ggtctggatg aacgaccact cgccgtcgac ccgctcgtgc
    11401 aactgttgcg cactaaaact ccgcgcccgc gcgaaggtct gggcccactc cgcctcggcc
    11461 aacgcccacg cggcgcggaa gccgtcggcg tcggtaggcc gcgcccgacc aggctgtgtc
    11521 gtcatggccg acaccttagg cgcgagcacc gacatcggtc gtcggaacca ccgtcacgca
    11581 gcattgcccc ggcgtcgggg tgagccgcgc ctggacgtca cccctgccca acgcgtcgag
    11641 cactccctgg acgaaatcgc gattgactcc gcacaccagt gccgtgtgct ccgccgccat
    11701 cgagtggaac gggcagttga ccaggcgcag ccccgcctcc tcctcccgag gctcgtagcc
    11761 caacccggcg agcacctggg gcaaccgatc cccgtccgtg ggcacggact cgaccacccg
    11821 gtatgccgcc cgctcggcgg ccgctcgcag cgcaccttcc aggggcacac cggcagcggc
    11881 ttgctcgacc gcatcggcga ggatgcggcc cgcgaggtcg taatgccggg gcggcaggga
    11941 gaccgcgaac tcctgggtcg agcgtcgata gagcttggct ggacgtccgg cccccgggcc
    12001 ggtgcgcccg gtgcgccgct ggaactccac gtcaagcagg ccgtcgtcgg ccaaccgatc
    12061 gaggtggaac ttcgcagtgt gcacggggac gccgaccgcc tcagcagcct cctcgcgccc
    12121 caccggcccg cggctggcga cgacgaagtc gtagagggcg cgccgcaccg gctcggcgag
    12181 cgcgccgatg ccctcaacgg tcgccgcgag gtcggtcatg agccatcctg atttctaaaa
    12241 gattctttga ttgactttag aacgggagga acgtaacgtc aaccctagtc tccgttagaa
    12301 aggggttcag atgtcccagg ccgccactcc cacggtcccc gcaggcgaag cgcccacggc
    12361 ataccccttg ctcggcccgc gccgcccccg ggtgacgggg cacgccccac tcgtggacct
    12421 tgggcgagcc gaggccgcgg ccaccgagct gctgcgggcc ctcgggctgc cgatcgacac
    12481 cgaggcgatg tgcgacaccc ctgggcggct gacccgggcc tatctcgagc tgctcagcgt
    12541 gcccgacttc gagatgacga ccttcgccaa cgacgagggc tatgacgagc tcgtcgtcgt
    12601 tgccgacatc cccgtgcggt cgttgtgtga gcaccacatg ctgccgttcg tcggggtcgc
    12661 gcacgtgggt tatgtgcccg gtgaccggct gctgggattg tcgaagttcg cccggaccgt
    12721 cgacttcttc gcccgtcgac cccagactca ggagcgactc accagtcaga tcgccgacca
    12781 cctggacgcg cacctgagcc cgcagggggt cggcgtcgtc atcagcgccg agcacacctg
    12841 catgacgttg cggggcgccc gcgccgcagg cacccgcacc cgcacctccg ccacccgcgg
    12901 agtcctgcgc aaccacctgg ccacccgcgc cgagttcctt tcgcgcgcag tcacattgga
    12961 ggactcccga tgaaccccat cgtcgtcgtc ggcgcaggct tggcgggcgc caccgcagtg
    13021 accgcgttgc gcgagggggg tcacgacggt ccgctcgtcc tgctcggcgc cgagagccat
    13081 ccgccctatg agcgcccgcc gctgtccaag ggctacttgc tcgggaacga ccccatcgag
    13141 agtgcgttcg tccacgatcg ccagtggtat gccgcgcacg acatcgacct gcgcaccggc
    13201 gccttcgtca ctgatctgga cctcgggcgg aagtccgtca gcctgctcga cggtaccgag
    13261 atcgcctacg accagctgct gttggcgacc gggagccggc cacggcggtt gccgctggcg
    13321 gacgactcgg gggtgcccac ggcatacctg cggacgatcg aggacagcga ccggatccgt
    13381 gcggcgttcg ggccggggca ccggatcgtc atcatcggcg ccggctggat cggtctggag
    13441 gttgcggccg cggcgcgcgc tgcagggacc gaggtgaccg tcttcgaggt ggcggagttg
    13501 ccgctgctgc gggtgctcgg gccgcgggtc gcgcaggcgt tcgccgacct gcaccgcgct
    13561 cacggggtgg acctgcggtt ggggactgcg gtcagtgccg cggacctggc gggcgccact
    13621 cttgtcgtcg tcggcgtggg ggtcgctccc gatgcgtcgc tcgcccaggg ggccgggctc
    13681 gccgtctcca acggcgtcct cgtcgatgcc accctgcgca ccagtcaccc ggacgtgtat
    13741 gccgtcggcg acatcgccaa ccacgaccat ccgttgttgg ggcggcgggt gcgggttgag
    13801 cactgggaca ccgcgatcga gcaggccaag gtcgcggcgc acaacctggt cggggggcag
    13861 gaggcagagg cttacgaccg gctcccccac ttcttcaccg accagtacga cctcggcatg
    13921 gaatacgtcg ggtatgccgc cgctgacgcc gacaccgagg tcgtcgtgcg gggcgatctg
    13981 ggggccgagc agtggcgggc ctattgggtg tccggcggcg tggtcgtcgc cggcatgcat
    14041 gtcaacgagt ggggcgccat cgacgagatc cgcgagcaga tcggccaccc gctcgcctga
    14101 ggccccgctc gcctgaggcc ccgctcgcct gaggccccgc tcgcctgaag ccccgctcgc
    14161 cgctcggccg tccgaggtcc cagtcgtctg agggccccac tcgcctgagg gccccgctcg
    14221 ccgctcggcc gccctggcct taagtgtggt cgaacgcggg ccagggaccg cgttcaaccg
    14281 cacctaagcc gcatccacag ggggtcaggc ggctggttcg gtgggcccgc gagcaagtcc
    14341 cgggtccggc ggtcgaccgc gtggctgggc ggtcgcattc aacgcccggt cgtaggaacc
    14401 gtgggcggtg tcccaagcga cacccgcggc ggtgacggct gctgtcaacc ggtcacgatg
    14461 cgccacggtg tgatggcgcc cgcacaacaa cgcggcgttc gacaggtcgc tccggccacc
    14521 atcaacccag tgagtgacat ggtgggcatc gcaccaatgc gccgggatcc cgcacccggg
    14581 gaacgtgcac gactgatcgc gcaaccacaa cgccttcacc tgacccggag taaacaaccg
    14641 cgccgtgcga ccccaatcga ggacctcact tggtccgcca agaacgaccg ggatgacctg
    14701 cgcgtcacag gccagcctgc ggaccgtctc gggagcgatc acggtgccgg actcactcga
    14761 cccgaccgcg acccccgcgg acaaccgctc agtcaactcc gccaacccca tcgtgacgac
    14821 gagagtggtc ttcaccccga ccgggggacg agcacccgcg gcggtcgcgc ggcggcatac
    14881 ctcaatgagc gcttggccac ggcgctgccg agcgggccga cgatccgggg caccatcggc
    14941 gtcgggggct ggcttggaca acgggccgat cgccgcctcc aggacagcct tgccttcggc
    15001 atcgaggact aggcggtagc tgaacgcccc cagctcgtcg cccaccggcg ctgacaacga
    15061 caccaacgct gccgccgcgt cctggtcggc ctgcaactgc cccggccacc catagtcggc
    15121 gatcaaccgg gctcgcaact cccgcaccgc ccgcaccccg tgacccgcgc ccatcgtcaa
    15181 catcgcctcg accaccgtgg gcaccgcctc cggctggagt tggggcttga gtcgctcggc
    15241 ttcccgaatg accgctaccg cgaccggcac gggcacccgg ccggaacacg ccgcgtcggt
    15301 caccggccga gtcgcccggc gcaggccggc ttcgaccact gccgcgacct gaccgccacc
    15361 accagccacc gcgagccccg gcgcatgctc cgcgacccac cccgtcaacg accccgcctg
    15421 actcgcagcg acctcacccc gggtgaatgc ctcccgcgtc acggcgaccc gaccggcccc
    15481 cgccagccct gccacctcgt ccagcagcgt caacactccc gccaactcgg caccttgcgc
    15541 ctgccacaac acctcagcaa gcccgctcag cacgtctcgg gcagcctgca gcgtcgtcac
    15601 ccgatcggtg agcgaaacgg cgcctcggct gtcctccata gaacaacagt acgaccgacc
    15661 accgacagcc ctccccacca ccaaaccagg cagccgggcc ctctaggtgt ggtcgaacgg
    15721 ggcccaggga ccgcgatcaa ccacacctca ggcagggctg cgggctagca tccggaaggt
    15781 gacggacatg cggctttcgc agcaggatgc gcggcggatc gccgtgcagg cccagctgct
    15841 caccgccgac cggccggcga acctcttcga ggtgctcgac cgactaaccc tcctgcaact
    15901 cgagcccacc aaggtcgtcg caccgaccca ccacctggtg ctctggagtc gcctcggccg
    15961 gagctactca cgcgacgagc tcgctgatgc tctcgccgcc cgcgaagtca tcgaggtgca
    16021 gctgcatctg agaccggcgt ggttcgtcgc cctgctgcgc gccgagatgg ccgcctggcc
    16081 cggcggaggc gcaggcgcag gcgccggtgc ctcgtccccg cccgagtggc agatccgcat
    16141 cgcggagtgg ctcaagacca acgacgcgtg ccgccgcgac atcctccacc gactcgacgc
    16201 cgagggagcc cagccggccg gcgccctccc tgacacgtgc gtcgtcccgt ggcgctcgac
    16261 cggctggacc aacgacaaga acgtccaacg catgctcgac ttcatggagc aacgcggcga
    16321 gatcgccgtc tcccaccggg cgcacgggca gcggcactgg gacctggccg agcgcgtcta
    16381 ccccgacgac cctccggtgc cggccgccga ggcccggcgc gagcgcgacg cgcttcgcct
    16441 gcacgccctc ggcatcgccc gagcccgggc caccgcccga caggtcgagc ccgacgacat
    16501 cggcgaggcc ggtgaacccg cagtcatcga gggggtgagg gggcgctggc gggtggacgc
    16561 ggcatacctc gacggcctgg gtgacgacac gttccggcca cgtgtggcgt tgttgtcccc
    16621 gctcgaccgt ctggtcttcg accgcaagcg gatggccgac ctcttcgact tcgactacaa
    16681 gctcgagatg tatcagccga aagccactcg ccggtggggc tattgggcgc tgccgatcct
    16741 cgtcggcgac cggctggtcg gcaaggtcga cgcgaccgcg gaccgcgagg cgggggtcct
    16801 gcgcgtcgac gccgtccacg aggacgagcc gttcgacgag ccgacggcca cgcagatccg
    16861 cgccgagctc gcctctctcg cggggtggct cggactcgac ctcgacgagc gcttctgaac
    16921 gtctcggtgc gggtgtatca ggggcgggtc caacgactca tcgcgggtaa ccccctagaa
    16981 aggcgtaccc gatgtcacgt ctgcgcagtc ccatcccctg gctcgccgcc cttctcaccc
    17041 ttgctctgag cctgttcgtc agcgttcccg ccttcgcggc tccgccgtca ctggccggcg
    17101 ctgacggcac aggcgagcgc acgtggtctc cccgagtctg cgccttcacc gacgacgaga
    17161 tcaaggccga agaggagacg atcaagaccg agcaggggac gatcatcctc aacgaccaca
    17221 tcctgtgtgc gctcgccaat gaatcccaac cgaagaaccc cgtcgagcag gccatcatcg
    17281 acgccttcct cgcccgaacc gggtttgacc ttgatgcggc ccgtgaacac cacctcggag
    17341 cggtcctctg ctccgacggc ggggtgggca tggtc
//
LOCUS       DAOMZY010000022        16970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_3024_length_16970_cov_5.571209, whole genome shotgun sequence.
ACCESSION   DAOMZY010000022 DAOMZY010000000
VERSION     DAOMZY010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 16970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16970
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3024_length_16970_cov_5.571209"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PLK64_02470"
     CDS             complement(<1..298)
                     /locus_tag="PLK64_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78635.1"
                     /translation="MSAVDPSGASASGRVGGEAERFVDLRLLLPALVAWVVVVILQWS
                     GSATGVVVGVAVVSGFGAAVALHRMRRSRHGHGRDVDGGRRARRGAGRHTRPGDG"
     gene            complement(295..1338)
                     /locus_tag="PLK64_02475"
     CDS             complement(295..1338)
                     /locus_tag="PLK64_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010551710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HPV78636.1"
                     /translation="MARTEELEQALSARAVALLERAGSTPAARNGWVPVPPPGWSRYA
                     APPGVPDAAPPDVAVRGDRSRGGGPPRRVGGRHRLPPPRVITVPAGLGGSRVRARAAA
                     VGALLAVVVVVGLIFALRVAAAARAAEPVPVAPSRTGVVARSVPMAFASGSIGRSGAM
                     GDEGATSADGAMGDASVGVEGSAGSGPIAGAEPGTIGRLLVVHVVGEVGAPGVVRVPE
                     GSRVVDVIRAAGGALASADLERVNLARAVVDGEQVHVPAPGEQILAGANGAGAGAGAP
                     AASGGSAPALINLNSADLAQLDTLPGVGPVLAQRILDWRAQHGRFTSVDELGEVSGIG
                     DTLLAQLTPKVSV"
     gene            complement(1443..2123)
                     /gene="plsY"
                     /locus_tag="PLK64_02480"
     CDS             complement(1443..2123)
                     /gene="plsY"
                     /locus_tag="PLK64_02480"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HPV78637.1"
                     /translation="MSGSHVVAGLPQLVAGPLDLGSGELWRTVAVVVVAGAASFLWGG
                     INPATLIGRTLGRDVTRAGSGNPGATNAGRVLGRKWGILVGVLDVAKGFVPAFLGAVF
                     GSVWLAYVLGVAAVLGHVFSPYLRGRGGKGVATALGAILGVHPWYAVALLAVFVLVFA
                     VSRWVALGSLLAAALMLVGGLLAAFGRLPGAIGLPTMIWAWVLGLVIVLRHRDNVISR
                     LRSLGRLE"
     gene            complement(2123..2995)
                     /locus_tag="PLK64_02485"
     CDS             complement(2123..2995)
                     /locus_tag="PLK64_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPV78638.1"
                     /translation="MGVAIVTDSTAYLTPAQVAEAGAPVRVVPLHVVIGGTERREGVD
                     VRVEDLAEALRRFVPVTTSRPAPAEFLQVYEELAAAGADSIVSVHISADMSATVQGAQ
                     LAAAQSPVPVTVVDSRVLGAAMGYAVLSAAAAAGAGADAETVAAVARSRAHDATVVFY
                     VHTLEHLRRGGRIGSASALLGSALAIKPILALADGHIAPIEKVRTSSKALARMVELAG
                     DAADRARTAGRIVDIEVQHLDFAARAAEVADALAQRVPDAGEIRIGELGAVVGAHVGP
                     GTLAITVAPRPESH"
     gene            complement(3013..5970)
                     /gene="leuS"
                     /locus_tag="PLK64_02490"
     CDS             complement(3013..5970)
                     /gene="leuS"
                     /locus_tag="PLK64_02490"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPV78639.1"
                     /translation="MSDETPYRYTAALADTIEQAWQDRWENDGVFHADNPAGPWAQPD
                     AVAARGPKLLVLDMFPYPSGAGLHVGHPLGYIATDVFSRFHRMQGRNVLHALGYDAFG
                     LPAEQYAVQTGQHPRKTTEDNIVIMRRQLRRLGLGFDDRRSYATIDEDYYRWTQWIFT
                     QIRESWYDPEAVRPDGSHGAARPIAELVAALESGERSVPESEGRAWTELSAREQAGVL
                     EHYRLAYPSEAPVNWCPGLGTVLSNEEVTNEGRSERGNFPVFRRNLRQWMMRITAYAD
                     RLADDLDRVEWPEKVKIMQRNWIGRSEGAMVTFPVTTAAGEAAGIEVFTTRPDTLFGA
                     TFMVLAPEHPLVDEIIPSAGWPEGTHEVWKGADAAAADSPAAAVAAYRRSTSRKSDVE
                     RQMDDRTKTGVFTGAFAANPLNSNPIPVFVADYVLMGYGTGAIMAVPAGDQRDYDYAT
                     EFELPIVDVIAPVGVSDWPGTVALGHSFTEAGVAINSSNDDVSLDGLGTVEAKARITA
                     YLQDKGIGRGTVNYRLRDWLFSRQRYWGEPFPVVWDEDGVAHSLPDSMLPLTLPDVPD
                     YSPKTYDPEDSGSEPEPPLSRVSEWVNVELDLGDGRGVRRFRRETNTMPNWAGSCWYY
                     LRYLDPTNSEALVDPANERYWIGPQANPVPGAPVGTRDPGGVDLYVGGVEHAVLHLLY
                     ARFWHKVLFDLGHLSSEEPFRTYFSQGYIQAPAFTDERGQYVQAEEVVETPGVHGAEA
                     TFTWQGQPVKREFGKIGKSLKNMVSPDEMYAVYGADTFRIYEMSMGPLELSKPWETRA
                     VVGSARFLQRLWRNVVDEITGECRVVELTETDLDPALRTLLHKTIAGVREDYAGLRFN
                     TAIAKLIELNNAVTKLPAPPRSLVEPLVQMVAPVAPHIADELWERMGRSGSLALGPFP
                     VADLALLVEDTVTCVIQIKGKVRDRVDVPADISESDLRELVLAREKVLAATADGIRTV
                     IVRPPSLVNVVPM"
     gene            complement(6162..6800)
                     /locus_tag="PLK64_02495"
     CDS             complement(6162..6800)
                     /locus_tag="PLK64_02495"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HPV78640.1"
                     /translation="MNIYAWVLLCAFTVAALADWAAVAQRRPDLESVAKPAVLVILIL
                     LAWLLRADSVDYGNQLLIGLVLCLIGDVLLLGRSDRHFLAGLIAFLLAHVAYIAAFRR
                     IPGEGPIWWGVIAVAVLGVVVILTTVLPIVRASGRDGIPLVVYALVVGGMAALAWATG
                     LPVVGIGATLFLASDALIAYDRFARPLAWGHVVVHITYHLGQLLIVLGMLRT"
     gene            6875..7264
                     /locus_tag="PLK64_02500"
     CDS             6875..7264
                     /locus_tag="PLK64_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HPV78641.1"
                     /translation="MAASTFEHRVTTADTAAVLGSGDLPVLATPRLINWFERATFATA
                     QTVVEAPQTTVGTVVRIEHLKASTVGAVITVSCSTPISDGRRLIFHVRALDEDGAEIA
                     VGEMHRRVVDSDRFMARFTEATPRPAD"
     gene            7293..8813
                     /locus_tag="PLK64_02505"
     CDS             7293..8813
                     /locus_tag="PLK64_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPV78642.1"
                     /translation="MSATRRIGARELLSTVLDEGSWVCWDTPPPPVAEPGSGYAADLA
                     AAAEAAGTDESVLTGEGRIRGRRVACVAGEFRFLAGSIGKAAAERITLAFERARHEQL
                     PLFAAPTSGGTRMQEGTPAFLGMVKISAAVAQFKAAGLPYIVYLRHPTTGGVLASWGS
                     LGHVTVAEPGAMIGFLGARVYEALYGRPFPEGVQVAENLHARGIIDAVVPVERLAEMA
                     ARTLSVLDARHTDQAPVPRAEASVPDPYAGLEPPASAWDSILLSRRTDRPGVKTLLKL
                     GASDVLLLRGTGQGEHDPGMLLALARFGGTPCIVLGQDRHRKTLEEPLGPGGLRVARR
                     GIRLAADLNLPLVSIIDTEGAALSKEAEEGGLAGEIARSLADLVMLPAPTLCVLLGQG
                     TGGGALAILPADRVICAQHAWLSPLPPEGASAIVHRTIERADEMAASQRVRSVDLRED
                     GIVDRIVPEHPDAASEPEAFCRRMAEAIHQEVDELWRADSRARVAARLERYRRMGT"
     gene            9012..9503
                     /locus_tag="PLK64_02510"
     CDS             9012..9503
                     /locus_tag="PLK64_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78643.1"
                     /translation="MPVIKALEARSHELVDEALGLLDHRLNPPYKQVDRDVLRRRLHQ
                     TMLTVTTALTERTPLPVIDYAREVARRCFNAGFLLEEVQTHFNALEETVWKFMVKEVD
                     AAHLPENLAMVASVIGTIKDELGRSYVELAAHHRAPAINTHKLFDGTQSVPRHPMSAK
                     AAR"
     gene            9649..10581
                     /locus_tag="PLK64_02515"
     CDS             9649..10581
                     /locus_tag="PLK64_02515"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HPV78644.1"
                     /translation="MSGQSVSRALTRLADLTAEAVGSAHGGVAAAVRGVLRLGRDRSV
                     VVVGRDGTSATMRLHESRFSGMDTIGSQLLSGSYEPTTRRVLEALVTSGATVVDVGAN
                     IGYFTVLSALLAGPTGRVIAFEPEPRNHAVLTDNVARNGLRQVMVVQAACADRVGQHE
                     LSINSSETGWHRLVAAGEVGRGKRVVVEVTTVDTIVGEAPVDVVKIDVEGHEGAVVTG
                     MAQTLADNPGITVILEHSPAQARLAGLDPLAPVTLLHQAGLRHTYVVREDKAVLEKID
                     VESLRSRDVKTGRSVNLVMRAQPWPADSAVPVVS"
     gene            10590..>11270
                     /locus_tag="PLK64_02520"
     CDS             10590..>11270
                     /locus_tag="PLK64_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPV78645.1"
                     /translation="MPGFDVSQFRAQFPSLTSGLAHFDGPGGTQTPYAVGAAVAATLT
                     GPLSNRGTTGVSETNAEAAVAGFRAAYADFLGVPERGVVYGRSATALTFDVGRAVAAS
                     WRPGDEIVVSRLDHDANVRPWLIFAERAGVTVRWLDFDPATSEIIWESVESAITERTR
                     LVAVTGASNLIGTIPPVAAIAARAHAVGALVWVDGVHFAAHHLVDVGAIGADFFVCSP
                     YKFLGTHCA"
     assembly_gap    11271..11370
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11371..11595
                     /locus_tag="PLK64_02525"
     CDS             <11371..11595
                     /locus_tag="PLK64_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HPV78646.1"
                     /translation="PTLLLTFPGRRSWDAYQFLSARGIQAPAGSFYAYEPFTRLALSD
                     THGLRVGLAPYTDDSDVQRLLDGLRAFLAS"
     gene            complement(11751..12659)
                     /locus_tag="PLK64_02530"
     CDS             complement(11751..12659)
                     /locus_tag="PLK64_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78647.1"
                     /translation="MEQAHRVGDTVPVFVQRRGTGRPTLRGEIVLEDLDPILERIASR
                     SGSNGPQSEVTQWILAQAARHLAEELTRDISPRPAGDSLPRVFSSLIGHEPFVKQILD
                     VSIHQMNRRWPQASDWYYDLIAYIMRPQRYARNFTDILGELPTWTQLTLGQLTKAFGE
                     FELSYGRDPLLYGLADVIETLWPDHPQVKANHERAQQLITQQWGSLYQFALPIYGVRL
                     RTGVTVDEVVWSIQALASWDMREEHIRLSPPRYADPIDGADRPHCSRASMIYLAGAVE
                     NLDGSLLSLEDLYRRTPVAVPGWPVG"
     gene            12871..14784
                     /locus_tag="PLK64_02535"
     CDS             12871..14784
                     /locus_tag="PLK64_02535"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPV78648.1"
                     /translation="MQQRFRGSISRARRTLICAGTTVALAATMTVAAAFTSPAAQAAT
                     PSYKVAVVGDSWISGEGLENYLSGTDTATNQCHRSTRGWTQQVIMPGHSLTIGRLVND
                     GSATMSLAFTACSGATIPNVLSTSKFGENPQLGRIPTDATHVFLSIGGNDLHFKERVE
                     HCANPIVNCLEEVGYLSNMLTEIDSLKPQLTTVLTKIRERASSARIILTGYARLFSPY
                     YMCLGAINSSEAAMFGTAAERLDQVQAQAAEDARRAGVDVSYVSLLSRFDGRGDCSLD
                     RWINPLVIGAPAAMHPNLKGHTEIVDLVRGPIAVATKAGAMKKAGVSMGLATSNVYCG
                     LRDGGCGQRYEKVTLFWSPATGAHYSSGQILGKYGTLGYENGAIGYPTNDRSCGLADS
                     GCVQSFQNGRIYSSASAGANWVHGTILTSYLALGAQTHLLAYPTSDEFVLGSGRKQNF
                     QGGAMYWSSATGAHFLRGQILDKYVALGQQNHVLGFPTKDRSCGLVDDGCYAVFSGGR
                     IYSYTGGAFYTRGQIMDRWLALGAQNSTLGYPDSDRVCNSTTDDCHQSFQDGALYSST
                     LYGAKWVHGLILNHYLVERADAGWLGSPTTDEKAWCGMRASFFEHGKITWQSGVGTKA
                     SSSTSTTCPSTPV"
     gene            complement(14927..14999)
                     /locus_tag="PLK64_02540"
     tRNA            complement(14927..14999)
                     /locus_tag="PLK64_02540"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14964..14966),aa:Ala,seq:ggc)
     gene            complement(15033..15701)
                     /locus_tag="PLK64_02545"
     CDS             complement(15033..15701)
                     /locus_tag="PLK64_02545"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPV78649.1"
                     /translation="MSGFPSGDTRKLVLVRHGRTDWNLAKRAQGHTDIPLDEVGHAQA
                     QAMAPFVAALRPSRIWSSDLARASQTAAYVAALTGVTVEVDARLREYSVGERSGLTRV
                     EFQERFPREYAAWLAEDETCLVPGAESTADVLARMVPALRSFLAALGPGEVGVVVTHG
                     ACLRAAMLELIGLPLSADAAFATMGNCTWSTLQEAEFGGRLRVAAYNEGVPAHDFASG
                     DEVG"
     gene            complement(15720..16127)
                     /gene="rsfS"
                     /locus_tag="PLK64_02550"
     CDS             complement(15720..16127)
                     /gene="rsfS"
                     /locus_tag="PLK64_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPV78650.1"
                     /translation="MSATPRAIDLARAAAVGASEKKAENIIAIDVSSQLALTDIFVIA
                     SATNERQVGAIVDEVEERVRLAGAKPLRREGGRDDRWVLIDFGDIVVHIQHQEERAFY
                     QLERLWRDCPTVELGLDEPVSAPAGRSSGDAAD"
     gene            complement(16124..16714)
                     /gene="nadD"
                     /locus_tag="PLK64_02555"
     CDS             complement(16124..16714)
                     /gene="nadD"
                     /locus_tag="PLK64_02555"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HPV78651.1"
                     /translation="MQRIGVMGGTFDPVHHGHLVAASEVQALLNLDEVVFVPTGQPWQ
                     KDGSAVSSAEHRYLMTVIATASNPKFTVSRVDLDRDGPTYTIDTLRDLRAQRPDAELF
                     FITGADALAQILTWKDVDELWSLAHFVGVTRPGHVLSDNGLPTDKVTLLEVPAMAISS
                     TDCRNRVGRGEPVWYLVPDGVVQYIGKHHLYHPRNP"
     gene            complement(16714..16908)
                     /locus_tag="PLK64_02560"
     CDS             complement(16714..16908)
                     /locus_tag="PLK64_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78652.1"
                     /translation="MSASVLPALVRAEGEVHRQLPMPAEMYGVVALVLFVLLLALLWS
                     FRGTAYKVRDRHSKPGGGHH"
BASE COUNT         2726 a         6078 c         5593 g         2473 t
ORIGIN      
        1 gcccatcccc aggtctcgta tgccgcccgg cgccccttct cgcccgccgc ccgccgtcga
       61 cgtcgcgccc gtgcccatgc cgcgatcgac gcattcggtg cagggcaacc gcggcaccaa
      121 agcctgagac gacagcgacg ccgacgacga ccccggtggc cgatccactc cattgcagga
      181 tgacaacgac cacccaggcg acgagggccg gcaacagcag gcgcagatcc acaaagcgct
      241 cggcctcgcc accgacgcgc cccgacgcgc tggcccctga cggatcgacg gcgctcacac
      301 gctcaccttg ggcgtgagct gcgccagcaa cgtgtctccg ataccgctca cctcccccaa
      361 ctcgtccacc gaggtgaacc ggccgtgttg agcgcgccag tcgaggatcc gctgggcgag
      421 caccggcccc acaccgggca acgtgtccaa ctgggcaaga tcggcggagt tgaggttgat
      481 cagcgcggga gcgctgccac cggaggccgc cggagcgcca gcacccgcgc ccgcgccgtt
      541 ggctccggcg aggatctgct cacccggcgc ggggacgtgg acctgctccc catcgacgac
      601 cgcgcgcgcg aggttgaccc gttcgaggtc ggcagaggcc aacgcccctc cggccgctcg
      661 gatcacatcg acaacccggg aaccctccgg cacccgcacg acccctggcg cgccgacctc
      721 accgacaaca tggacgacga ggagccgccc gatggtgccc ggctctgcgc cggctatggg
      781 gcccgacccc gccgaaccct cgacacccac tgaggcgtcg cccatcgccc cgtccgcact
      841 cgtcgctccc tcgtcgccca tcgcccccga gcgcccgatc gaccccgacg cgaacgccat
      901 cggcaccgac cgcgccacca cccccgtccg gctcggcgcc accggaaccg gctcagccgc
      961 ccgcgcagcc gccgccaccc gaagcgcgaa gatcagcccg acaaccacca ccacagccag
     1021 cagcgccccc acggccgctg cgcgcgcccg caccctcgac cctcccaagc cggcgggcac
     1081 ggtgatcact cggggcggag gcagccgatg ccgtccgccc actcgccgcg gtgggccgcc
     1141 cccgcgtgac cggtcaccgc gcacggccac gtcgggcggc gcggcatcag gaacgcccgg
     1201 gggtgcggca tacctcgacc agccaggcgg tgggaccggg acccaaccgt tacgggccgc
     1261 cggcgtcgag ccagcgcgct ccagcaacgc caccgcccgc gccgacaggg cctgctccag
     1321 ttcctcagtg cgcgccatgc ccggacggta tgccgcccgc tcaccttccc gtcaggcccc
     1381 caccatcgcc tgtggacagt gggacgagcg tcagcaccct tgtggacaac cccacaaccg
     1441 acctactcca ggcgccccaa cgaccgcagc cgggagatga cgttgtcccg atggcgcagc
     1501 acgatgacca gccccaacac ccaggcccag atcatcgtcg gcagcccgat ggcgcccggc
     1561 agccggccaa aggcggcgag cagcccgccg acaagcatga gcgctgccgc gagcagcgac
     1621 cccaaggcca cccagcgcga gaccgcgaag acgagcacga agacggcaag gagagcgacg
     1681 gcataccagg ggtgcacgcc gaggatggca cccaaggcgg tcgcgacccc cttgccaccg
     1741 cgcccgcgca ggtagggcga gaagacgtgc cccagcaccg cagcgacccc gagcacatag
     1801 gcgagccaca cggagccgaa gaccgccccg aggaaggccg ggacgaaacc cttggccacg
     1861 tcgaggaccc ctacgaggat cccccatttg cggcccagca cgcggccggc gttggtcgca
     1921 ccggggttgc ccgagcccgc gcgagtgacg tcgcgaccca gcgtccgccc gatgagagtg
     1981 gctgggttga tgccacccca gaggaacgac gcggcccctg ccacgaccac cacggcgacg
     2041 gtccgccaca gctcaccgga ccccaggtcg aggggcccgg ccaccaactg cggcagaccg
     2101 gcgaccacgt gcgacccgct cactagtggc tctccggccg aggcgccacc gtgatcgcca
     2161 gcgttcccgg acccacatgc gcgccgacga cggcgcccaa ctcgccgatc cggatctcgc
     2221 ctgcgtccgg cacccgctgc gccagcgcgt cggcgacctc ggcggcccgt gccgcgaagt
     2281 ccaggtgctg gacctcgatg tccacaatcc ggcccgccgt gcgggctcga tccgctgcgt
     2341 cgccggccaa ttcgaccatc cgcgccaacg ccttgctcga cgtgcgcacc ttctcgatcg
     2401 gggcgatgtg tccgtcggcg agagcgagga tcggcttgat cgccagcgcc gagccgagca
     2461 gggccgacgc cgagccgatc cgcccaccgc gccgcagatg ttcgagcgtg tggacgtaga
     2521 agaccaccgt cgcgtcatgt gctcgcgaac gcgccaccgc cgcaacggtt tccgcatcgg
     2581 ctccggcacc cgccgcggcg gcggccgaca acacggcata ccccatggcg gccccgagca
     2641 cccgcgagtc gacgaccgtc accggcaccg gcgactgcgc ggcagcaagc tgcgcaccct
     2701 ggaccgtagc cgacatgtca gccgagatat gcacggagac aatggaatcc gcacccgcag
     2761 cggccaactc ctcatagacc tggaggaact cagccggcgc tggccgcgac gtcgtcacgg
     2821 ggacgaatcg ccgcagcgcc tccgccagat cctcgacccg cacgtcgaca ccttcacgac
     2881 gctcggtgcc accaatgacg acgtgcaggg ggacgacccg caccggcgcg ccagcctcgg
     2941 ccacctgagc cggcgtgagg tatgccgtgg aatcggtgac gatcgcgacg cccatccggg
     3001 tgcctcctga cctcacatgg ggacgacgtt gaccagcgac ggcggccgga cgatgacggt
     3061 gcgaatgccg tccgccgtgg cagccaacac cttctcacgg gcgagcacca actcgcgcag
     3121 gtccgactcg gagatatcgg ccggcacgtc cacccgatcg cggaccttgc ccttgatctg
     3181 gatgacgcag gtgaccgtgt cctcaaccag gagagcgagg tcggcgaccg ggaacggccc
     3241 gagcgccagc gacccgctgc gacccatccg ctcccacaac tcgtcggcga tgtgcggcgc
     3301 gacgggcgcc accatctgca ccaacggctc gacgagcgac cgcggcggcg caggcaactt
     3361 cgtcaccgcg ttgttgagct cgatgagctt ggcgatggcc gtgttgaacc gcagccccgc
     3421 atagtcctca cgaacaccgg caatcgtctt gtgcagcaac gtccgcaggg cggggtcgag
     3481 atcggtctcg gtcagctcga ccacccggca ctcacccgtg atctcgtcga cgacgttgcg
     3541 ccacaaccgc tgcaggaagc gcgccgagcc gacgacggct cgggtctccc acggcttgga
     3601 caactccagc ggacccatcg acatctcgta gatgcggaac gtgtcggccc cgtagacggc
     3661 atacatctcg tccgggctga ccatgttctt cagcgacttg ccgatcttgc cgaactcgcg
     3721 cttgaccggc tggccctgcc acgtgaacgt cgcctccgcg ccgtgcaccc cgggggtctc
     3781 cacgacctcc tcggcctgga catactgccc acgctcatcg gtgaatgccg gggcctggat
     3841 gtagccctgc gagaagtagg tccggaacgg ctcttcgctg gacaggtgac ccaggtcgaa
     3901 aagcaccttg tgccagaacc gggcatagag caggtgcagc accgcgtgct cgactccacc
     3961 gacatagagg tcgacgccac ccgggtcgcg cgtccccaca ggagcaccag ggaccggatt
     4021 agcttgcggt ccaatccaat accgctcatt ggccgggtcg accagtgcct cggagttggt
     4081 cgggtcgagg tagcgcaggt agtaccagca ggatccggcc cagttgggca tcgtgttggt
     4141 ctcgcggcgg aaccgccgca ccccgcgccc gtcgcccagg tccaactcga cgttgaccca
     4201 ctcgctcacg cgggacagcg gcggctcggg ctcgctgccc gagtcttccg ggtcataggt
     4261 cttcggcgag tagtccggga catcgggcaa cgtcaacggc aacatcgagt cgggcaacga
     4321 gtgcgcgacc ccgtcctcgt cccacaccac ggggaacggc tcgccccaat agcgctgccg
     4381 gctgaacagc cagtcgcgca ggcggtagtt gaccgtccct cggccaattc ccttgtcctg
     4441 caggtatgcc gtgatcctcg ccttcgcctc gaccgtcccc aacccgtcga gcgacacgtc
     4501 atcgttggac gagttgatgg cgaccccggc ctcggtgaac gagtgcccca gggcgacggt
     4561 gccgggccag tccgacaccc ccacgggggc gatgacatcg acgatcggca actcgaactc
     4621 agtggcgtag tcgtagtcgc gctgatcgcc ggccggcacc gccatgatcg cgccggtccc
     4681 gtaacccatg agcacgtagt cggcgacgaa caccggaatc gggttgctgt tcaacgggtt
     4741 tgcggcgaag gccccggtga acacccccgt cttggtgcgg tcgtccatct gccgctcgac
     4801 atcgctcttg cgcgaggtgg agcgtcggta tgccgcgacc gccgccgccg gggagtccgc
     4861 cgccgccgcg tcggcgccct tccacacctc atgggtgcct tcgggccagc ccgcgctcgg
     4921 gatgatttcg tcgaccagcg gatgctcggg agcgagcacc atgaaggtcg ctccgaacag
     4981 cgtgtcgggc cgggtcgtga agacctcgat gccggcggcc tcgcccgcag ccgtcgtcac
     5041 cgggaaggtc accatggcgc cctcggaccg gccgatccag ttgcgctgca tgatcttgac
     5101 tttttcgggc cactcgaccc ggtccaggtc gtcggccaac cgatcggcat acgccgtgat
     5161 ccgcatcatc cactgacgca ggttgcgccg gaacaccggg aagttgcccc gctcggagcg
     5221 tccttcgttg gtgacctcct cgttggacaa cacggtgccc agaccgggac accagttgac
     5281 tggcgcctcc gagggatagg ccaaccggta gtgctccagc acgccggcct gctcgcgtgc
     5341 tgacaactcg gtccatgccc gcccctccga ctcgggcaca gaccgctccc ccgactccaa
     5401 ggccgccacc aactcggcga tggggcgagc cgccccatgc gagccgtcgg ggcgcactgc
     5461 ctcggggtcg taccaacttt cccggatctg ggtgaagatc cactgggtcc atcggtagta
     5521 gtcctcgtcg atcgtcgcgt aggagcgtcg gtcgtcgaat cccagtccca gacgccgcaa
     5581 ttggcggcgc atgatgacga tgttgtcctc ggtcgtcttg cgaggatgct gaccggtctg
     5641 cacggcatac tgctccgcgg gcagcccgaa ggcgtcgtag cccagggcat gcaggacgtt
     5701 gcgcccctgc atccggtgga agcgcgagaa gacgtcggtc gcgatgtagc ccaaaggatg
     5761 cccgacgtgc aggcccgctc ccgaggggta ggggaacatg tccaggacga gcagtttcgg
     5821 accccgcgcg gcgacagcgt ccggctgcgc ccacggcccg gcggggttgt ccgcgtggaa
     5881 caccccgtcg ttctcccaac ggtcctgcca cgcctgctcg atcgtgtcgg ccagcgcggc
     5941 ggtgtagcga tacggcgttt cgtcgctcat cgtgctctct tgtccttcgg caactgtcgt
     6001 ggtcgtgggc cggtcgaacg ccggtgcctg gtgggcaaaa agaaacccct cacgcaggag
     6061 gggctgccgc gctgacgtcc gactactcag cgcggcccgg taaggagcag actccgtgcc
     6121 acggccccag ggtagcgcac ccccatgcgc acctacctgc ctcaggtgcg cagcatcccc
     6181 aggacgatga gcaactgccc aaggtggtag gtgatgtgca cgaccacgtg cccccaggcc
     6241 agcggccggg cgaagcggtc ataggcgatg agcgcgtcgc tcgcgaggaa cagcgtggcc
     6301 ccgatcccga cgacgggcag cccggtcgcc caggcgaggg cggccattcc gcccaccacc
     6361 agcgcataga ccaccagggg tatgccgtcc cggccactcg cccggacgat cggcagcacc
     6421 gtggtgagga tcaccacgac ccccagcacg gcgacggcga tcactcccca ccagatcggc
     6481 ccctctccgg ggatccggcg gaaggccgcg atataggcca catgggccag gaggaaggcg
     6541 atcaacccgg cgaggaagtg ccgatccgat cgcccgagca gcagcacatc gccgatgaga
     6601 cacagcacga ggccgatgag caactggttg ccgtagtcga ccgagtccgc tcgcagcagc
     6661 caggccagca ggatgaggat gacaaggacc gcgggcttgg ccacggactc gaggtcgggt
     6721 cggcgctggg cgaccgcagc ccagtccgcg agcgctgcga cagtgaacgc gcacagcagc
     6781 acccaggcat agatgttcat tggcacacac cctctcgcat cagggcgcat tgccgggcca
     6841 gcggcgcgct cggggccgcc ctagactcga tgacatggca gcgtccacgt tcgagcaccg
     6901 ggtgaccacg gccgacacgg ccgccgtcct cggcagcggc gatctccccg tgctggccac
     6961 cccgcgcctg atcaactggt tcgagcgggc caccttcgcc accgcgcaga cagtcgtgga
     7021 ggcaccacag accaccgtcg gcacggtggt gcgcatcgag cacctcaagg ccagcacggt
     7081 cggggcggtg atcaccgtgt cgtgctccac cccgatcagt gacggccgcc gactgatctt
     7141 ccacgtgcgg gccctcgacg aggacggtgc cgagatcgcg gtgggcgaga tgcaccgccg
     7201 cgtcgtcgac tccgatcgat tcatggcgcg gttcaccgag gcgacgccgc gccccgccga
     7261 ctagccacac cctcacgacg aaaggcccgg ccatgagcgc aacgcggcgc atcggggcaa
     7321 gggagttgct gagcactgtt ctcgacgagg gcagttgggt ctgttgggac actccgccgc
     7381 ccccggtggc tgagcccggc agcgggtatg ccgccgacct cgccgccgcg gccgaggccg
     7441 ccggcaccga cgagtcggtc ctcaccggcg aagggcgcat ccggggccga cgtgtggcct
     7501 gtgtcgccgg tgagttccgc ttcctcgccg ggtcgatcgg caaggctgcc gccgagcgga
     7561 tcacgctggc cttcgagcgc gctcgacacg agcaactgcc cctcttcgcc gctccgacct
     7621 ctgggggcac gcggatgcag gaaggcacgc cagccttcct cggcatggtc aagatctcgg
     7681 ccgccgtcgc ccagttcaag gccgccggat tgccctacat cgtctatctg cgccacccga
     7741 cgaccggcgg cgtgctggcc tcgtgggggt ccctgggcca cgtcacggtc gccgagccgg
     7801 gagcgatgat cgggttcctc ggcgcccgcg tctacgaggc gctctacgga cggccgttcc
     7861 ccgagggcgt ccaggtcgcc gagaacctcc acgcccgcgg catcatcgac gcggtggtgc
     7921 cggtcgagcg gctcgccgag atggcggcgc ggacgctctc ggtgctggac gcgcggcata
     7981 ccgaccaggc gccggtcccc cgcgccgagg cgagcgtgcc ggacccgtat gccggtctcg
     8041 aaccgcccgc gagcgcgtgg gactccatcc tgttgtcccg tcgcaccgac cgaccgggcg
     8101 tgaagaccct gctcaaactc ggcgcgtccg acgtcttgtt gttgcgtggg accggacagg
     8161 gcgagcacga ccccggaatg ttgttggcgc tggcccgctt cggcgggacg ccgtgcatcg
     8221 tcttgggtca ggatcgacac cgcaagacac tcgaggaacc gctcggaccg ggtgggttgc
     8281 gggtggcacg tcgtggcatc cggctggccg ccgacctcaa cctgcccctg gtgtcgatca
     8341 tcgacaccga gggcgcggca ctgtccaagg aggccgagga gggcgggctg gccggcgaga
     8401 tcgcccggtc cctcgccgac ttggtcatgc tcccggcgcc cacgctgtgc gtcctgctcg
     8461 ggcagggcac cggtggcggc gccttggcga tcctgcccgc cgaccgggtg atctgtgccc
     8521 agcatgcgtg gctgtccccg ttgccccccg agggagcctc ggcgatcgtg catcgcacga
     8581 tcgagcgggc cgacgagatg gcggcgtcgc agcgggtccg ctcggtggat ctgcgcgagg
     8641 acgggatcgt cgaccgaatc gttccggagc accccgatgc cgcgtcggag cccgaggcgt
     8701 tctgccgacg gatggccgag gctatccacc aggaggtcga cgagttgtgg cgggctgatt
     8761 cccgggctcg ggtggccgcg cgactggagc gctaccgccg gatgggcacc tagttcaccc
     8821 gctgttcacg cacccgcggg acctctggcc cggcgcttct gacgccggtc cgtcgtaccc
     8881 ttggttcaac gggcacagcc gccaggggca gcacgacgct gtgcgcttcc ccgctcggct
     8941 ggcccaacgt gtgcggtatg ccgccgaggc agcctcacag cgacgcccca cccgcgaaag
     9001 gcagaactca cgtgccggtc atcaaggccc ttgaggcccg ttcccacgag ctcgtcgacg
     9061 aggccctggg cctgctcgac caccggctca acccgcccta caagcaggtc gaccgtgacg
     9121 tgctgcgccg ccggctgcac cagacgatgt tgacggtgac gaccgcgctc accgagcgca
     9181 cgcccctgcc ggtcatcgac tacgcccgcg aggtcgcgcg ccgttgtttc aacgccgggt
     9241 tcctgctgga agaggtgcag acgcacttca acgcgttgga agagaccgtg tggaagttca
     9301 tggtcaaaga ggttgacgcc gcccacctgc cggagaacct cgccatggtc gcctccgtca
     9361 tcgggacgat caaggacgag ctgggccggt cctacgtcga gttggccgcc caccaccggg
     9421 cgccggccat caacacccac aagctcttcg acggcaccca gagcgtcccg cgtcacccga
     9481 tgtccgccaa ggctgctcgc tgatcttctg gcgacgccct tggcccgcac tggtcggcac
     9541 gcaccgactg acgggctgac gtgatcccgg tgtttccggc tggctgggcc gagcgctagc
     9601 gttgtccttc gtggggaacg tggccagccg cgaaacgaag gagcttcagt gtccggtcag
     9661 tcggtatctc gcgcgctgac gcgcctggcc gacctcaccg ctgaagctgt cggctcggcc
     9721 catggtggcg tcgccgccgc cgtccgaggg gtccttcgcc tgggtcgtga tcgcagcgtt
     9781 gtcgtggtgg ggcgcgacgg gacgtccgcc accatgcggc tgcacgaatc ccgcttctcc
     9841 gggatggaca cgatcggcag ccaattgctt tctggcagtt acgaacccac gacccgtcga
     9901 gtgttggaag ccctcgtcac ctccggggcc accgtggtcg atgtcggcgc caatatcggc
     9961 tacttcaccg tcttgtcggc cctgctcgcc ggacccaccg gtcgcgtgat cgcgttcgag
    10021 cccgagccgc gcaaccacgc ggtcctcacc gacaacgtcg cccgcaacgg gctgcgccag
    10081 gtgatggtgg tgcaggcggc gtgcgccgac cgggtcgggc agcacgagtt gtcgatcaac
    10141 tcctccgaga ccgggtggca ccgactggtc gccgccggtg aggtcgggcg cggcaaacgg
    10201 gtcgtcgtgg aggtgaccac cgtcgacacc atcgtcggcg aagcgccggt cgacgtcgtc
    10261 aagatcgacg tcgagggcca cgagggcgcg gtcgtcaccg ggatggccca gaccttggcc
    10321 gacaatccgg ggatcaccgt catcctcgag cactcccccg cccaggcccg cctcgccggg
    10381 ctcgacccgc tcgcaccggt gacgctgttg caccaggcgg ggctgcggca tacctatgtg
    10441 gtccgcgagg acaaggcggt cctcgagaag atcgacgtcg agtccctgcg ctcgcgcgac
    10501 gtcaagaccg ggcgctcggt caatctcgtc atgcgcgccc agccctggcc cgcggactcc
    10561 gcggtgccgg tcgtctccta ggaggtccga tgcccggctt cgacgtgagt cagttccgcg
    10621 cgcagttccc ctccctgacc tcggggctcg ctcacttcga cgggcccggc ggaacgcaga
    10681 cgccgtatgc cgtgggagcc gccgtcgcgg ccaccctcac cgggccgctg tccaaccgcg
    10741 gcaccacggg ggtgtccgag accaatgccg aggcggcggt cgcagggttc cgggcggcat
    10801 acgcggactt cctgggggtg cccgagcgag gggtcgtcta cgggcgaagc gcgacggcac
    10861 tgaccttcga cgtggggcgc gcggtggcgg cgagctggcg tccgggtgac gagatcgtcg
    10921 tctcacgcct ggaccacgac gccaacgtcc ggccgtggct gatctttgcc gagcgcgccg
    10981 gcgtgacggt gcgctggctg gactttgatc cggcgacgtc cgaaatcatc tgggagagtg
    11041 tcgaatccgc gatcacggag cggacccgac tggtggcggt gaccggggcg tccaacctca
    11101 tcgggacgat cccgccggtg gcggcgatcg cggctcgggc ccatgcggtg ggcgcgctcg
    11161 tgtgggtgga cggggtgcat tttgcggcgc accacctcgt cgacgtcggg gcgatcgggg
    11221 ccgacttctt cgtgtgctcg ccctacaagt tcctcgggac gcactgcgca nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccgaccctct tgctcacctt ccccggccga
    11401 cgctcgtggg acgcctacca gttcctgtcc gcgcgcggca tacaggctcc tgcggggtcc
    11461 ttctatgcct acgagccgtt cacgcggttg gcgttgtccg acacccatgg cctgcgggtg
    11521 gggttagcgc cctacaccga cgactccgac gtgcagcggc tgttggacgg cctgcgcgcc
    11581 ttcctcgcct cctgagtcag ccgaccggcc gcccagggac ggcggctcgg gaggccgcgc
    11641 gcacacggca taccaatggt cagcccactc gggcgccgtg agcactgatg cccgccgcga
    11701 tcgcgaccat tgtcattcgg tgtgcaggtg actgccaacc cgcgcgggcc tcagcccacg
    11761 ggccacccgg ggacggcgac cggcgtgcgg cggtagaggt cctccagact caggaggctc
    11821 ccgtcgaggt tctcgaccgc gccggcgagg tagatcatcg acgcgcgcga gcagtgcggc
    11881 cggtcggcgc cgtcgatggg gtcggcgtag cggggcgggg acagccggat gtgctcctcg
    11941 cgcatgtccc aggaggcgag ggcctggatc gaccagacga cctcgtcgac ggtcactccc
    12001 gtgcgcagcc ggacgccgta gatggggagg gcgaactggt agagcgaccc ccactgctgg
    12061 gtgatgagtt gctgggcgcg ctcatggttg gccttgacct gcgggtggtc cggccagagc
    12121 gtctcgatga cgtcggcgag gccgtagagc agcgggtcgc ggccatagct caactcgaac
    12181 tcgccgaagg ccttggtgag ctggccgagg gtcagctggg tccaggtcgg cagctccccg
    12241 aggatgtcgg tgaagttccg ggcataacgc tgggggcgca tgatgtaggc gatgaggtcg
    12301 tagtaccagt ccgaggcctg gggccagcga cgattcattt ggtggatgct cacgtcgaga
    12361 atctgcttga cgaagggctc gtgcccgatg agcgaggaaa agacccgcgg caacgagtcc
    12421 cccgccgggc gcgggctgat gtcacgggtg agctcttcgg cgaggtgacg cgcggcctgc
    12481 gcgaggatcc actgggtgac ctcgctctgc ggtccgttgg acccgctgcg actggcgatg
    12541 cgctcaagga tcgggtcgag atcctccagg acgatctctc ctcgcagggt cggccgcccg
    12601 gtgccgcgtc gctgcacgaa gacgggaacc gtgtcgccca cccgatgcgc ttgttccata
    12661 ggccaattca agcgaaaccg accctcgggc gcttgcccat ccagtgggtg actgacgcgg
    12721 gtggtcagcc gtgcacgaag cctcggcacc cccggggtca cgtccccggg gggacggtgc
    12781 ccccttatcc ctcgtggcgc cgctccggtt ggttgtcgcc aggcctcttc gactctctcc
    12841 gaggcccgat ccgaccgagg aaggattcgc atgcaacaac gattcagggg ctcgatcagt
    12901 cgagctcgtc gaacgctgat ctgtgcaggc accaccgtcg cgctcgcggc caccatgacg
    12961 gtggcggcgg ccttcacctc gccggcggcg caggctgcga cgccgtccta caaagtggcc
    13021 gtcgtcggcg actcgtggat ctcgggcgag gggctggaaa actacctcag tgggaccgac
    13081 acggccacga accagtgcca ccggtcgacc aggggctgga cccagcaggt catcatgccg
    13141 gggcactccc tgacgatcgg gcggctcgtc aatgacgggt cagcgacaat gagcctggcc
    13201 ttcaccgcct gcagcggggc caccatcccc aacgtcctct ccacaagtaa gttcggcgag
    13261 aacccccagc tcgggcgcat ccccactgac gcgacgcacg tcttcctcag catcggcggc
    13321 aacgacctgc acttcaagga acgagtcgag cactgcgcca atccgatcgt caattgctta
    13381 gaagaggttg gctacctctc caacatgctc accgaaatcg acagcctcaa accccagttg
    13441 acaacggtcc tcaccaaaat cagggagcgc gccagctcgg cgcggatcat cctgactggc
    13501 tatgcccggc tgttctcgcc gtattacatg tgcttggggg cgatcaactc cagtgaggca
    13561 gccatgttcg ggacggcagc cgagcggctc gatcaagtcc aggcacaggc ggccgaggac
    13621 gcgcgcaggg ccggggtcga cgtgtcgtac gtgtccttgc tcagccgctt tgacggccgc
    13681 ggcgactgtt ccttggatcg atggatcaac ccgctggtca tcggcgctcc ggcggcaatg
    13741 caccccaacc tcaaagggca caccgaaatc gtcgatctcg tccgcggacc gatcgcggtc
    13801 gcgaccaagg ctggtgcgat gaagaaggct ggcgtctcga tgggcctggc cacgagcaac
    13861 gtctactgcg ggctgcgcga cggtggctgc ggccagcgct acgaaaaggt caccctcttc
    13921 tggtccccag ccacaggggc gcactactcg tcgggtcaga tcctcggcaa gtacggcacc
    13981 ctcggctacg agaacggcgc catcgggtac cccacgaacg accgctcctg cggcctcgcc
    14041 gacagcgggt gcgtccagtc cttccagaac gggcggatct actcgtccgc gtcagcgggt
    14101 gccaactggg tccacggcac gatcctgacc agttacctcg cgttgggcgc ccagacgcat
    14161 ctgctggcct acccgacgag cgacgagttc gtcctggggt cggggcgcaa gcagaatttc
    14221 cagggtggcg cgatgtattg gtcgtcggcc accggggcgc acttcctgcg cggtcagatc
    14281 ctcgacaagt acgtcgcgct cggccagcag aaccacgtgt tgggcttccc caccaaggac
    14341 cggtcgtgtg ggctcgtcga cgacggctgt tatgcggtct tctccggcgg acggatctac
    14401 tcctacaccg gcggcgcgtt ctacacccga ggccagatca tggaccggtg gctcgcgctg
    14461 ggcgcccaga acagcaccct gggttacccc gacagtgacc gagtctgcaa cagcacgacg
    14521 gacgactgcc accagtcctt ccaggacggg gcgctctatt cgtcaaccct gtacggcgcc
    14581 aagtgggtcc acggcctgat tctcaatcac tacctggttg aacgcgcaga cgctggctgg
    14641 ctgggttctc ccaccacgga tgagaaggcc tggtgcggca tgcgggcgtc gttcttcgag
    14701 cacggcaaga tcacctggca gagcggcgtc ggcaccaagg cgagttcttc cacgtcgacc
    14761 acctgcccct cgacaccggt ctgaccgaca cgcctccgcg cacgaacggg caggctcgca
    14821 ccaggggggc ctgcccgttc ttgtggccga cgagaggcgg tcgccaagaa ccgacgaagc
    14881 cctgcccacc gatcaccggg ggcagggctt caacgtcaac cgattgtgga gctgagggga
    14941 ttcgaacccc tgaccccctc catgccatgg aggtgcgcta ccaactgcgc tacagccccg
    15001 ctccccggct cgcaccgggc aacccgtgaa tcctacccaa cctcgtcgcc ggacgcgaaa
    15061 tcgtgtgccg gcacgccctc gttgtaggcc gcgacccgca gccgaccgcc gaactcggcc
    15121 tcctgcaacg tgctccacgt gcagttgccc atcgtcgcga acgccgcgtc ggcgctcaac
    15181 ggcagcccga tgagctccag catcgccgct cgcaggcacg ctccgtgggt gacgaccaca
    15241 ccgacctcac ccggccccag cgccgccaag aacgaccgca gagcgggcac catccgggcc
    15301 agcacgtcgg cggtggactc ggcccccggg accaggcacg tctcgtcctc agccagccac
    15361 gcggcatact cccgcgggaa ccgctcctgg aactcgacgc gcgtcagccc ggagcgctcc
    15421 cccacggagt actcccgcag tcgcgcgtcc acctccacgg tcacaccggt cagcgccgcc
    15481 acgtatgccg cggtctgact cgcgcgcgcg aggtccgagg accagatccg gctggggcgc
    15541 agggccgcga cgaacggtgc catcgcctgc gcctgcgcgt gcccaacctc gtcgaggggg
    15601 atatcggtat ggccctgggc acgcttggcg aggttccagt cggtccggcc gtggcgcacc
    15661 agcacaagtt tgcgcgtgtc ccccgacggg aacccgctca tccgggtcgg cacagccggt
    15721 cagtcggcgg cgtcgccgct cgaacgcccc gcaggcgccg agaccggctc gtcgagcccc
    15781 aactccacgg tcgggcagtc ccgccacaac cgctcgagtt ggtagaaggc gcgttcctcc
    15841 tggtgctgga tgtgcacgac gatgtcgccg aagtcgatga ggacccagcg gtcgtcacgc
    15901 ccgccctcgc ggcgcagggg cttggcgccg gccagccgca cgcgctcctc gacctcgtcg
    15961 acgatcgcac cgacctggcg ttcgttggtc gccgaggcga tgacgaagat gtcggtgagg
    16021 gccagctgac tggagacgtc gatggcgatg atgttctcgg ccttcttctc ggacgcgccg
    16081 actgctgcgg ctcgggcgag gtcgatggct cggggtgtgg cgctcacggg ttcctagggt
    16141 ggtagaggtg gtgcttcccg atgtattgga ccacgccatc gggcacgagg taccacacgg
    16201 gctcgccgcg gccgacccgg ttgcggcagt cggtggacga gatggccatg gccggcacct
    16261 ccaacagggt gactttgtcg gtcggcaggc cgttgtcgct gagcacgtgc cccggccggg
    16321 tgaccccgac gaaatgggcg agcgaccaga gctcgtcgac gtccttccac gtgaggatct
    16381 gggcgagggc gtcggcgccg gtgatgaaga acagctcggc atcgggccgc tgggcgcgca
    16441 gatcgcgcag ggtgtcgatc gtgtaggtcg ggccgtcccg gtccagatca acccggctga
    16501 cggtgaactt cgggttcgag gcggtcgcga tgaccgtcat gagatagcga tgttcggcgg
    16561 acgacaccgc cgacccgtcc ttctgccacg gctgccccgt ggggacgaag acgacttcgt
    16621 cgaggttcag cagggcctgg acctcactcg cagcaaccag gtgaccgtgg tggacgggat
    16681 cgaacgtccc gcccatcacc ccgatccgct ggatcagtgg tggcccccgc cgggcttgga
    16741 gtggcggtcg cgcaccttgt aggcggtgcc gcggaaggac cacaacagcg cgagcaggag
    16801 cacgaagagc accagggcga cgacgccgta catctctgcc ggcatcggca actggcggtg
    16861 gacctcgccc tccgcacgga caagggcggg aagcacgctc gcggacatgg ccacatgcta
    16921 gcgggtgacc cccctccccg tgcgcgcggc ataccttctt ggctgtcacc
//
LOCUS       DAOMZY010000023        16446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_3168_length_16446_cov_6.703618, whole genome shotgun sequence.
ACCESSION   DAOMZY010000023 DAOMZY010000000
VERSION     DAOMZY010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 16446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16446
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3168_length_16446_cov_6.703618"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PLK64_02565"
     CDS             complement(<1..446)
                     /locus_tag="PLK64_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPV78653.1"
                     /translation="MLAVQTWELTKRFGSQTVVDGIDLAVPAGSVYGFLGPNGSGKTT
                     TIRMLLGLIGPSAGRSELLGLPVPGQAEAALHRVGALVEGPAFHPYLSGRANLARLDV
                     ADRRADPRTSRARVDAALDRVGLLAAAEKRYRAYSLGMRQRLAIAGT"
     gene            complement(457..1695)
                     /locus_tag="PLK64_02570"
     CDS             complement(457..1695)
                     /locus_tag="PLK64_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78654.1"
                     /translation="MTASPLRSRFGRWLVPAAAVVLVAGVSLAGSRMASADSDLAPRT
                     AAELLADLQRARLDHLSGTVVQTADLGLPSLPGVSGMSGSVGAGASELTSIISGSHTW
                     KVWYGGENQQRLALVGTLGESDIIHNGSDVWIWSSKAQTATHLVIDPAKAAAAKSEAG
                     KTGGPLMTAGPTGTGLPPTPEDAAKAALAAIDPTTRAEVGPAVVVAGRTAYELVLTPK
                     DSTTLVAQVRLAVDGERHLPLRVQVFSTKQSTPAIEVGFTSIDFSAPEARQFEFTPPP
                     GTTVTEGMPEGLGDKALGDKALGDKAAGMPTEPSAGAAAAKAAAPRAVGTGWSRVVVA
                     TMPATTDAAPGGGTAADGGLGQLQSLLGALPQVSGAWGSGRVLEGTLFSLVLADDGRV
                     ALGAVAPDALYAALAAPAAG"
     gene            1801..2454
                     /locus_tag="PLK64_02575"
     CDS             1801..2454
                     /locus_tag="PLK64_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPV78655.1"
                     /translation="MRVLVVEDERGMAAALHRGLSAEGFVVDVCRDGPSGLEAARFGG
                     YDAVLLDLMLPGMSGYTVVRTLREEANWVPVIVLSAKDGEYDQADALDYGADDYLTKP
                     FSFVVLLARLRALVRRDPTPRPPTVSAHGIRLDPATREVTVDGAAVELALREFAVLER
                     LLTAAPAVVSKEELLDAVWGDAAEGINVVEVYVGYLRRKLGRERIETVRGVGYRIAP"
     gene            2451..3857
                     /locus_tag="PLK64_02580"
     CDS             2451..3857
                     /locus_tag="PLK64_02580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPV78656.1"
                     /translation="MTAGPRWFQRLSIRWRLMTVGLVGLTVALVAAGSLLYAVLSATL
                     ANTLGAEANSAAREVATLVEQGRLSDPVPVSGALVVQVLDSSQRVIAGSLVADRLTPL
                     LTADEVRRALAGEQLTVPGSRSGLSGRLHVAAVSAGPGSAPVTVIAGVPTGEVDVSLG
                     LVRTLLFVGLPLLLLALAAVAWRVIGAALAPVEALRLGAERIGADARPRRRRATVDRL
                     PIPVADDEIHSLATTLNGMLDRLDGLAERQQAFLDDAAHELRSPLASLRTQLEVAEHV
                     GDGGSLPADLLPDVERLSRLVDDLLVLARSGNGEASPSLETVDLRPLLGEIVSRYAAA
                     RVPVTLTDVAPGATAIEVPGNRADLRRVVTNLVDNAVRHASTAVTLSVGADPSGTWLR
                     VTDDGHGIPLADRERVFERFTRLDEARDRDSGGTGLGLPIARELMHRNGADVTLTEAA
                     PGVIAVVAWPRSTEKPSS"
     gene            complement(3935..6388)
                     /locus_tag="PLK64_02585"
     CDS             complement(3935..6388)
                     /locus_tag="PLK64_02585"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HPV78657.1"
                     /translation="MTGGVGDGETWWGRLGGARSPGKVAPCRTQPCDNSPRPSQGSGR
                     TALKEAMRTVLRETLWWIRKAPVTTGIVLVALAVGIAMNALWVPAPSSGLADTYGWGL
                     PAFARGEWWTLLVGAFIAPAPWMYLLILPLIAVGGGFLEYKYGAWRMLAALFVTHVAS
                     VLIVSLIIYLLAPLGVGWAVRLVDVRDGGLSNAGFGAIGAMTAALAPSWRRRARTGLF
                     LYALVMVLYVGFIWDLTHLVALLVGFAVGPLVVKRPYARFSYALTTQEKRVLVAQIVV
                     FNIITLVVTYLVPQGGLLESSDTYQRDAGSTVVVFGLSIFLGLLAYGTYAGRKIAWRL
                     LLTLTILLFLIGLILAETPAGVFGVFVNAVLLVLLVVFRRVFTIKGDRVVRSGIYKRL
                     AALALGLFMLNAVAIFAMRAQFSPEPDFGMAVVESIWLIFGGSSLDFVPQTPAASFLT
                     QGIGYVWGFALLGGIGALVFTTLRDRDGRGQFAEYDRLLHEHGSTSIGWMARWPGMSY
                     WVDPARTAGFAYRLENNIAIVLSDPVGSPDAVATSLGGFTEMCDRRGWRPVFYSVTES
                     FRALAAQHGYQAIVVGEDTVIDLPDLAFTGKSWQSVRTAVNQAGKLGISMRPIRYAEA
                     TAGMRVQLQAIAASWSDDKSLPPLGFTLGTLAEASDPEVIMHVAVDAEGTVHGMTSWM
                     PVYGQGTVDGWTLDIMQRGLSEDTMRGVVEFLIAESAMAFKQEGYRFISLSASPLSRG
                     EGQTGPIEALLDMVAKRLEPLYGFQSLHSFKAKFKPRFEPMYLCYRDEAQLPAISIAI
                     IKAYLPDNALRGVVASKIK"
     gene            complement(6444..8156)
                     /locus_tag="PLK64_02590"
     CDS             complement(6444..8156)
                     /locus_tag="PLK64_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPV78658.1"
                     /translation="MTNATENYRQARDQLLALRGDHARAVAEFRWPDVGDTFNWACDW
                     FDVVARGNDKPALILVEEDGSSQTRSFDEMARRSDQVAAWLAAQGVGKGDPVLLMLGN
                     QVELWDCMLAVMKLGAIIMPTTTAAGSTDLIDRIARGGARFVVTNPDQTAKLDEVPGD
                     YGRISIGEADGWADLRAAYLTPAEPTPHPGSAPGDPVLLYFTSGTTSRPKLVEHTQVS
                     YPVGHLATMFWLGLQPGDVHLNISSPGWAKHAWSCFFAPWIAEATIFVYNYARFDAAA
                     LLGQLREHEVTSFCAPPTVWRMLINADLSGGPGALREVIGAGEPLNPEVIDQVAKAWG
                     LSLRDGYGQTEMTAAVGNTPGSAVKPGSMGRPLPGQPVVLVDPNTGERVEGAGAEGEL
                     CFDLSARPLLLMTGYQGDEVRNAEAMEGGWYHTGDVASIDADGYITYVGRTDDVFKAS
                     DFKISPFELESVLIEHPAVAEAAVVPAPDEVRLAVPKAYLMLAPGYAADEDTARSILK
                     YAREHLQPWQRVRRIEFAELPKTISGKIRRVELRSREEQVATGDIKPSGTEWRDDMFP
                     ELKG"
     gene            complement(8261..8899)
                     /locus_tag="PLK64_02595"
     CDS             complement(8261..8899)
                     /locus_tag="PLK64_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78659.1"
                     /translation="MRRLPGIFLALCVAVALASCGVFGASSAAPTATVFVTVPEPEPT
                     GSAPTGSRAPAATTTAVVGPTIDAAGHGVTTWDSPSGNIVCAAFPNDGAPGFEVRCDV
                     MQHTWTLPPKPASCEFDWGHGTFLTTKSGITCVSDAIAGSDVVGSDGTWWNGQPGSQV
                     VTVEGRKAVALAYGASLKFGDILCTSATDGMHCTNTTTKAGFDISRAAYSLR"
     gene            complement(8987..9403)
                     /locus_tag="PLK64_02600"
     CDS             complement(8987..9403)
                     /locus_tag="PLK64_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78660.1"
                     /translation="MWSSPAGDIVCSASRKSSSAPFEIRCDALEQVWTVPAKPDSCAF
                     DWGHGTYLNTKAGLACVNDSLVGSEAVGAPGAWWNGKPGSQVMTLPGRKAVTLAYGAN
                     FTFGPLACSSQLDGMHCTNSETKAGFDINRTQYTLR"
     gene            10189..10323
                     /locus_tag="PLK64_02605"
     CDS             10189..10323
                     /locus_tag="PLK64_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78661.1"
                     /translation="MTRDFVVERMNTHAGQTLAGVFGGLMRLAGDPPYEFVGQPSQFA
                     "
     gene            complement(10773..11816)
                     /locus_tag="PLK64_02610"
     CDS             complement(10773..11816)
                     /locus_tag="PLK64_02610"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPV78662.1"
                     /translation="MPSAAVNVGIVGATGQVGGVVLRILAERNFPVGQLRLFASSRSA
                     GSTITWQGRDIVVEDASTADPTGLHIAIFSAGATTSKAQAPRFAAAGVTVIDNSSAWR
                     MDPEVPLVVSEVNPAAAKNPPKGIIANPNCTTMAAMPVLKPLADEAGLKRLIVSTYQA
                     VSGTGLAGVQELATQVRRALESGDIEGLAHDGRAVDLGAPVKYLAPIAFDVVPVAGSV
                     LDDGSEETDEERKLRNESRKILDLPDLRVSGTCVRVPVFTGHSLSINAEFERPITPAR
                     ARELLANAPGVELADVPTPLQAAGADPSYVGRIREDQSVDGGRGLALFVSNDNLRKGA
                     ALNTVQIAELLIS"
     gene            complement(11819..13093)
                     /locus_tag="PLK64_02615"
     CDS             complement(11819..13093)
                     /locus_tag="PLK64_02615"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPV78663.1"
                     /translation="MSVIVQKYGGSSVADAEGVKRVARRIVETKQAGHDVCVVVSAMG
                     DSTDELIDLANKVTPNPPARELDMLLTAGERISMAVLAMAIADLGSEARSFTGSQAGV
                     ITDAEHGKARIIDVTPGRITEALAAGHIAIVAGFQGVSQTSKDITTLGRGGSDTTAVA
                     LAAALKADVCEIYTDVDGVFTADPRVLPTARKLSVISSEEMLDLAANGSKILHLRCVE
                     YARRHGIAIHVRSSWSGHEGTWILENPYGEGSTMEAPIIAGVAHDRSEAKITIVGVPD
                     EAGKAADIFTAVADAGINIDMIVQNVSAAATGRTDISFTLPSSDGPKAVQTLQSLRAA
                     IGFDELRFDDQIGKLSLVGAGMRTNPGISAKLFRALAHAGINIEMISTSEIRISVVTR
                     ADQLDAAVRAAHTAFDLDSAEGEAIVYGGTGR"
     gene            complement(13198..13836)
                     /locus_tag="PLK64_02620"
     CDS             complement(13198..13836)
                     /locus_tag="PLK64_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HPV78664.1"
                     /translation="MPDADRSGMPPAEPDAVSASAEPGSEFDDFAEFAGLADDSALQA
                     RAYVTAVTEVASGSSPDIAIPLLLLATAQLLLAGSRLGAVQDVVLDERYEPDPGPDAD
                     VEPLRDNLANLFEGIDDYADLVDPVTQAELTKGSLSNDMTEICLALAHGLSHYDAGRR
                     VEALWWWQFSYLSTWGERAAASLRVLQSIIAHLRLDADEDVVADAEFDALHP"
     gene            complement(13829..14428)
                     /gene="recR"
                     /locus_tag="PLK64_02625"
     CDS             complement(13829..14428)
                     /gene="recR"
                     /locus_tag="PLK64_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPV78665.1"
                     /translation="MYEGIVQDLIDELGRLPGIGPKSAQRIAFHLVQADPADVARLVQ
                     VLAEVKDKIRFCELCGNVAEATLCRICGDSRRDQTAICVVEEPKDVAAIERTREFRGR
                     YHVLGGAISPMDGIGPEDLRIRELMTRLADGGIVEVIIATDPNIAGEATATYLTRTLA
                     PLGIRVTRLASGLPVGGDLEYADEVTLGRAFEGRRTVHA"
     gene            complement(14458..15411)
                     /locus_tag="PLK64_02630"
     CDS             complement(14458..15411)
                     /locus_tag="PLK64_02630"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HPV78666.1"
                     /translation="MTTGDRLRGGIFGLLVGDALGVPYEFHPATQIPATDEIEMVPPA
                     GFARAHAGIAPGTWSDDGAQALVLLESLLRVGHLDVDDVGRGLVAWDDEGRYAVDRHV
                     FDIGIQTGIAIRSLRAGVPAASSGPTDSRANGNGSLMRVLPLALWHRGSDADLAADAM
                     AQSAVTHGHLRSQVCCVLYCLLARRLLQGMPPAQAWPAARAAFDEVYPAGSPEHDELV
                     THVFPDGAAYEIRGGGYVVESMRAAVALLLADDPLDYERVVKAAISLGEDTDTTACIA
                     AGLAGIVAGEGGIPLRWRTALRGRDLVEPLADALVSWRGSA"
     gene            complement(15497..16168)
                     /locus_tag="PLK64_02635"
     CDS             complement(15497..16168)
                     /locus_tag="PLK64_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPV78667.1"
                     /translation="MFYRVGATLLQPVSRLLYRPTITGIENVPPKGGVILASNHLSFI
                     DSFAIPLVAPRKVSFLAKSDYFTGSGPRGLLSRGFFSSVGAIPVDRNSSRAAQESLDL
                     ALEVLQAGGAFGIYPEGTRSRDGRLYRGRTGVGWLALTAGVPVVPVGLRGTDQIQPVG
                     SRWPRLAKVSVAFGPPIHPETYAGMPAGRARRELTDDVMDAIALLSGQERADTYNEVP
                     TLDPS"
     gene            complement(16234..>16446)
                     /locus_tag="PLK64_02640"
     CDS             complement(16234..>16446)
                     /locus_tag="PLK64_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78668.1"
                     /translation="ELARAKEATRRTMDERHHRSDPAPAPRATSPQSDLGAASLDDEV
                     VQEGAEIGQAVIERMLGGTVLGDTPT"
BASE COUNT         2603 a         5660 c         5734 g         2449 t
ORIGIN      
        1 gtgcccgcaa tggccagccg ctggcgcatg ccgagcgaat aggcccggta gcgcttctct
       61 gcggcggcga gcaggccgac tcgatcgagc gccgcgtcga cccgcgcacg ggacgtgcgc
      121 gggtcggccc ggcggtcggc gacatcaagg cgggccaggt tggcccgacc ggacaggtag
      181 gggtggaagg ccggcccttc gacgagggcg ccgacccggt gcaacgccgc ctcggcctgg
      241 ccgggcaccg gcagcccaag cagctccgag cgcccagccg atgggccgat cagcccaagc
      301 agcatccgga tcgtcgtcgt cttgcccgac ccgttggggc cgaggaaccc atagaccgac
      361 ccggcaggca ccgcgaggtc aatgccgtcg accaccgtct gagagccgaa ccgtttggtc
      421 aactcccacg tctgaactgc cagcatccgc gcgccgtcag ccggccgcgg gcgcggcgag
      481 ggcggcatac agggcgtcgg gggcaacagc cccgagcgcg acgcggccgt cgtcggcgag
      541 gacgagcgag aacagcgtcc cttcgaggac ccggccactg ccccacgcac ccgagacctg
      601 cggcagcgcg cccaggaggc tctgcagttg acccagccca ccgtcggcgg cggttccccc
      661 gcccggtgcg gcgtccgtgg tggcaggcat cgtggccacg acgacccgcg accagccggt
      721 gccgaccgcg cgcggtgcgg cggccttggc ggcggcggcg ccagcgctag gctcggtcgg
      781 cataccagca gccttgtctc ccaaagcctt gtctcccaaa gccttgtctc ccaagccttc
      841 cggcatccct tcagtcaccg tcgtcccggg cggcggggtg aactcgaact gccgagcctc
      901 cggcgccgag aagtcgatcg acgtgaaccc gacctcgata gccggcgtgg actgcttggt
      961 cgagaagacc tgcacccgca agggcaggtg tcgttcgccg tcgacggcca ggcgcacctg
     1021 ggcgacgaga gtggtcgaat ccttgggcgt gagcaccagc tcgtatgccg tgcgccccgc
     1081 gacgaccacc gcgggcccga cctcggcccg ggtcgtcggg tcgatggccg cgagcgccgc
     1141 cttggcggcg tcctcaggcg tggggggcag cccggtgccc gtcggcccgg ccgtcatcaa
     1201 cggcccgccg gtcttcccgg cctcactctt ggctgcggcg gccttcgcgg ggtcgatgac
     1261 caggtgcgtg gcggtctggg ccttgctcga ccagatccac acgtcgctgc cgttgtggat
     1321 gatgtccgac tcgcccagcg tgccgaccag ggccaggcgc tgctggttct cgccgccgta
     1381 ccagaccttc caggtgtggc tgccgctgat gatcgaggtc agctcggacg caccggcacc
     1441 gacgctcccc gacatgccgc tcaccccagg cagggacggc agcccgaggt cggcggtctg
     1501 cacgacggtc cccgacaggt ggtcgaggcg agcccgctgc agatctgcga gcagttcggc
     1561 ggcggtgcgc ggggcgaggt cgctgtcggc cgaggccatc cggctaccgg ccagcgagac
     1621 gccggcaacg aggacgacgg cggcggcggg cacgagccaa cggccgaaac gggaacgcaa
     1681 cggggaagcg gtcatgaccc caggatgcaa caccccacct gagaggaccc tgagacggca
     1741 taccggcggc tcgggcccgg cgccgacgat ctgtcgctcc gctcgtgtca tcgtgtcctt
     1801 gtgcgggtgt tggtggtcga agacgagcgc gggatggccg ctgcgctgca ccgcggcctg
     1861 agcgcggagg gcttcgtcgt cgatgtatgc cgcgacggcc cctccggtct ggaggccgct
     1921 cggttcgggg gctacgacgc ggtgctgctg gacctcatgc tgccgggcat gtcgggctac
     1981 accgtggtgc ggaccctgcg cgaggaggcc aactgggttc cggtcatcgt cctgtcggcg
     2041 aaagacggcg aatacgacca ggccgacgcc ctcgactacg gcgccgacga ctacctcacc
     2101 aaaccgttct ccttcgtcgt cctgctcgcc cggctgcggg ccttggtccg gcgggatccg
     2161 acgccgcgcc cgcccacggt gtcggcgcac ggcatacggc tggatccggc gacgcgtgag
     2221 gtgaccgtcg acggggcggc cgttgagctc gcgctgcgcg aattcgccgt gctggagcgg
     2281 ctgttgacgg ccgccccggc tgtggtgagc aaggaggagc tgctcgacgc ggtgtggggc
     2341 gacgcggccg agggcatcaa cgtcgtcgag gtctacgtcg gctacctgcg ccgcaaactc
     2401 gggcgcgagc ggatcgaaac ggtgcgcggc gtcggctatc ggatcgcgcc atgacggccg
     2461 ggccgcgctg gttccaacgg ctgtccatcc gctggcggct catgaccgtc gggctcgtcg
     2521 gcctcaccgt cgcgctcgtg gccgccggca gtctcctgta tgccgtgctg tccgccaccc
     2581 tcgccaacac ccttggcgcg gaagcgaatt cagcggcccg agaggtcgcc accctggtcg
     2641 agcagggccg attgtctgac ccggtgcccg tgtcaggggc gctcgtcgtg caggtgttgg
     2701 actcctctca gcgcgtcatc gccgggtcgc tggtcgccga ccggctcacg ccgttgctca
     2761 ccgccgacga ggtgcggcgc gctctggccg gcgagcaact gacggtgcct ggctcgcggt
     2821 ccggtctctc ggggcggctg catgtcgcgg cggtgtcggc cggcccgggg tctgcgccgg
     2881 tgaccgtcat cgccggcgtg ccgaccggtg aggtcgacgt gagcctcggc ctggtccgaa
     2941 cgctgctctt cgtcgggctg cccttgctgc tgctcgcgct cgccgccgtc gcctggcggg
     3001 tcatcggtgc cgccctggcc cccgtcgagg cgctgcgcct cggggccgag cggatcggcg
     3061 ccgacgctcg gccccgtcgg cgccgtgcga cggtcgaccg cctgccgatc cccgtcgctg
     3121 atgacgagat ccactctctg gcaacgactc tcaacggcat gcttgatcga ctggacgggc
     3181 tcgccgagcg ccagcaggcc ttcctcgacg acgcggccca cgagttgcgc agcccgctgg
     3241 cctccctgcg cacgcaactg gaggtggccg agcacgtcgg ggacggtggc tcgctgccgg
     3301 ctgacctgct gcccgacgtc gaacggttga gccggctcgt cgatgacctg cttgtgttgg
     3361 cgcgctccgg caacggcgag gcctcgccga gcctggaaac cgtcgacctg cgccccctgc
     3421 tgggcgagat cgtcagccgg tatgccgccg cccgagtccc cgtgaccctc accgatgtcg
     3481 cgccgggcgc cacggccatc gaggttcccg gcaatcgggc cgacctgcgg cgagtggtga
     3541 ccaaccttgt cgacaacgcc gtccgacacg cctcgacggc ggtgaccctc tcggttggag
     3601 ccgacccgag cgggacctgg ctgcgcgtga cagacgacgg gcacggcata cccctggctg
     3661 accgggagcg cgtcttcgag aggttcacgc gccttgacga agcccgcgat cgagacagcg
     3721 gcggcaccgg gttgggtctg ccgatcgcgc gagagttgat gcaccgcaac ggcgccgacg
     3781 tcactctgac cgaggcagca cccggcgtga tcgccgtcgt ggcctggccg cggtcaaccg
     3841 aaaagccctc aagctaaccg accgcgagct ggaccagacg gactcggcag ccggctccca
     3901 gcggtgcggc ccacctcctg agcgcgctgc cgtcctactt gatcttcgag gcaacgacgc
     3961 cgcggagtgc gttgtcgggc aagtaggcct tgatgatggc gatcgagata gccggaagct
     4021 gggcttcgtc ccggtagcac aggtacatcg gctcgaaccg cggcttgaac ttggctttga
     4081 agctgtgcag gctctggaac ccgtagagcg gctccagccg cttggccacc atgtcaagaa
     4141 gcgcctcgat cgggccggtc tgcccctcac cccgactcag cggcgacgcc gacaggctga
     4201 tgaaccggta accctcctgc ttgaacgcca tcgcgctctc ggcgatcaag aactcgacaa
     4261 cgccacgcat cgtgtcctcg gacaggccgc gctgcatgat gtcgagcgtc cagccgtcca
     4321 ctgtgccctg gccgtagacg ggcatccagc tggtcatccc gtgcacggtg ccctcggcgt
     4381 cgacggcgac atgcatgatc acctcaggat cggacgcctc cgccagcgtg cccagggtga
     4441 agcccagcgg gggcagcgac ttgtcgtcgg accaactcgc ggcgatcgcc tggagctgaa
     4501 cgcgcatccc ggcggtggcc tcggcgtacc tgatgggacg catgctgatc ccgagcttgc
     4561 ccgcctgatt cacggccgtc cggacgctct gccacgactt tccggtgaac gccaggtcgg
     4621 gcagatcgat gacggtgtcc tcgccgacca cgatcgcctg gtagccgtgc tgcgccgcca
     4681 gcgcgcggaa ggactcggtc acgctgtaga acaccggacg ccaaccgcgc ctgtcacaca
     4741 tctcggtgaa gccgcccagg ctcgtggcca cggcgtccgg gctgccgacg ggatcgctca
     4801 ggacgatcgc gatgttgttc tccaaccggt aggcgaagcc agcggtcctg gctgggtcca
     4861 cccagtagct catgccgggc caacgcgcca tccagccgat cgaggtgctg ccatgctcgt
     4921 ggagaaggcg gtcgtactca gcgaactggc cacgcccgtc cctgtcgcgc agggtggtga
     4981 acaccagcgc tccgattcct ccgagcagcg cgaaacccca gacatatccg atgccctggg
     5041 tcaggaagct cgcggccggc gtctgtggga cgaagtcgag agagctaccg ccgaagatca
     5101 gccagatcga ctcgacgacc gccatgccga agtccggctc gggcgagaac tgtgcgcgca
     5161 tcgcgaagat ggccaccgcg ttaagcatga acaggccgag ggcgagcgcc gcgagacgct
     5221 tgtagatgcc cgatcggacg acccggtcgc ccttgatcgt gaagacgcgc cggaagacga
     5281 cgaggagaac gaggaggact gcgttgacga agacgccgaa gacccccgcc ggagtctccg
     5341 caaggattaa gccgatgagg aagagcagaa tcgtcagcgt cagcagaagc cgccacgcga
     5401 tcttgcggcc cgcgtacgtt ccgtaggcca gcagacccag gaaaatcgag agaccgaaca
     5461 ccacgacggt gctgcccgcg tcccgctggt acgtgtcgct cgactccaac aggccacctt
     5521 gtggcacgag gtacgtgacc accagggtga tgatgttgaa gaccacgatc tgggcgacca
     5581 ggacgcgctt ctcctgggtc gtcagcgcgt agctgaacct cgcatagggg cgtttgacca
     5641 ccagcgggcc gacagcgaag ccgaccagca acgcaaccaa gtgggtcaga tcccagatga
     5701 acccgacgta cagcaccatg accagggcgt acaggaacag gccggtgcga gcacgtcgcc
     5761 gccaactcgg ggcgagggcc gcggtcatgg caccaatcgc cccgaagccg gcgttggaga
     5821 gcccgccgtc tcggacgtcc accagcctga ccgcccaccc gacacccagg ggcgccagca
     5881 ggtagatgat caggctgacg atcagcaccg aggcgacgtg cgtgacgaag agggcggcga
     5941 gcatccgcca ggcgccgtac ttgtactcaa ggaagccgcc gccgacggcg atgagcggca
     6001 ggatcagcag gtacatccaa ggcgccggtg cgatgaacgc ccccaccaga agcgtccacc
     6061 attcaccgcg ggcaaaggca ggcagacccc agccgtaggt atccgccagg ccggacgaag
     6121 gggcgggcac ccacagtgcg ttcatagcga tgccgacagc gagcgcgacc aggacgattc
     6181 ccgttgtcac gggcgccttt ctgatccacc acagcgtttc ccggagtacc gtcctcatag
     6241 cttccttcag tgccgtgcgc cccgagcctt gggatgggcg cggagaattg tcgcacggct
     6301 gcgtccggca cggagccacc ttcccaggtg accgcgcacc gcccagtctc ccccaccacg
     6361 tctcgccgtc gccgacgccg ccggtcacct tgcctggatc tgatggattc tcccggcacc
     6421 tcacccctgg ctcaaggcca gatctacccc ttgagctccg ggaacatgtc gtcgcgccac
     6481 tcagtgccgc tgggcttgat gtcgccggtc gccacctgct cctcccgcga gcgcaactcg
     6541 acccggcgga tcttgcccga gatggtcttg ggcaactcgg cgaactcgat ccgccggacc
     6601 cgttgccacg gctgcaggtg ctcacgggcg tatttgagga tcgagcgcgc ggtgtcctcg
     6661 tcggcggcat acccgggcgc gagcatgagg taggccttgg ggacggcgag gcggacctcg
     6721 tcgggcgcgg ggacgacggc cgcctccgcc accgccgggt gctcgatgag gaccgactcc
     6781 aactcgaacg ggctgatctt gaagtcgctg gccttgaaca cgtcatcggt ccggccgaca
     6841 taggtgatgt aaccgtcggc gtcgatgctg gcgacgtcac ccgtgtggta ccagccgccc
     6901 tccatcgcct ccgcgttgcg gacctcgtcg ccctgatagc ccgtcatcag caacagcggc
     6961 ctggccgaca ggtcgaagca gagctcccct tctgctccgg caccctcgac ccgctcgcct
     7021 gtgttggggt cgacgaggac gaccggctgc cccggcaacg gccgacccat cgaccctggc
     7081 ttgactgccg agcctggcgt atttcccact gccgcagtca tttcggtctg gccatagccg
     7141 tcccgcaggg acagccccca ggccttggcg acctggtcga tgacctcggg gttgagcggc
     7201 tcccccgctc cgatgacctc ccgcagcgcc cccggcccac ccgacaggtc ggcgttgatg
     7261 agcatccgcc agaccgtcgg cggcgcacag aagctcgtca cctcatgctc gcgcagttgc
     7321 ccgagcagcg cggcggcgtc gaagcgcgcg tagttgtaga cgaagatcgt cgcctcggcg
     7381 atccacggcg cgaagaagca cgaccacgcg tgctttgccc accccggcga ggagatgttg
     7441 aggtggacgt cgccgggctg cagccccagc cagaacatcg tcgccagatg ccccaccggg
     7501 taggacacct gggtgtgctc gaccaacttg ggccgactgg tcgtcccgga ggtgaagtag
     7561 agcaacacgg ggtcaccggg cgcgctcccc gggtgggggg tcggctcggc aggcgtgagg
     7621 tatgccgccc gcagatccgc ccagccgtcc gcctcgccga tgctgatccg gccatagtcg
     7681 ccgggcacct cgtcgagctt ggccgtctgg tcggggttgg tcacgacgaa gcgagccccg
     7741 cctcgcgcga tccggtcgat gaggtcggtc gatcccgcgg cagtcgtcgt cggcatgatg
     7801 atcgcgccga gtttcatcac ggccagcatg cagtcccaca actcgacctg gttgccgagc
     7861 atgagcagca ccgggtcgcc cttgcccaca ccctgagcgg cgagccaggc agcgacctgg
     7921 tccgagcgcc gggccatctc gtcgaagctg cgcgtctggc tcgacccgtc ctcttcgacc
     7981 aagatgaggg ccggcttgtc gttgccgcgg gccaccacgt cgaaccagtc gcaggcccag
     8041 ttgaaggtgt cgccgacatc gggccagcgg aactccgcca ccgcgcgggc atggtcacca
     8101 cgcagcgcga gcaactggtc acgcgcttgc cggtagttct cggtcgcatt cgtcatgggc
     8161 tccactgtgc ccaagccggg ggccgctcgc taccggaacc ggctgtggcg cttgcgacac
     8221 ggcatacccc cgtcgacggt gcgccacgcg agtggtgacc tcagcgcagg gagtatgccg
     8281 cgcggctgat gtcgaagccc gccttcgtcg tggtgttggt gcaatgcatg ccgtcggtcg
     8341 cggaggtaca gagaatgtcg ccgaacttca ggctcgctcc gtaggcgagg gcgaccgcct
     8401 tgcggccctc gacagtcacc acctgggagc cgggctgacc gttccaccac gtgccgtccg
     8461 agccgacgac gtccgacccg gcgatggcgt cactgacaca ggtgatgccg gacttcgtgg
     8521 tgaggaacgt tccgtggccc cagtcgaact cgcagctggc cggcttgggc ggcagcgtcc
     8581 aggtgtgctg catgacatcg caccgcacct cgaagccggg cgccccgtcg ttggggaagg
     8641 ccgcgcaaac gatgttgccg ctagggctgt cccacgtcgt caccccgtgg cccgcggcgt
     8701 cgatggtcgg gccgacgacg gcggtcgtcg tcgcggcggg tgcccggctg ccggtcggcg
     8761 cgctccccgt cggctccggc tcagggacgg tcacgaagac ggtcgcggtc ggcgccgccg
     8821 aactggcgcc gaacacgccg cagctggcca gagcgacggc aacgcacagc gcgaggaaga
     8881 tcccggggag acgtctcacg gcatcgatcc tggcacaccc tcaaacgtcg atcagacaac
     8941 caacaatgtc gtgaggtcat caatggcgcg ccaggcggtg gtggcctcag cgcaaggtgt
     9001 actgcgtacg gttgatatcg aagccagcct tcgtctcaga gttggtgcaa tgcatgccat
     9061 ctagctgcga cgaacaagcc agcggcccga acgtgaagtt cgcaccatag gcgagggtga
     9121 ctgccttgcg cccaggcagg gtcatcacct gagagccagg tttcccgttc caccacgcac
     9181 cgggtgcccc cactgcttcg gagccgacga gcgagtcgtt cacgcaggcc aggccagcct
     9241 tggtattgag gtaggttccg tgcccccagt cgaacgcgca cgagtcgggc ttcgcgggca
     9301 ccgtccagac ctgctcgagc gcatcgcaac ggatttcgaa tggcgcgctc gaagacttcc
     9361 tggacgccga gcaaacgatg tccccggccg ggctcgacca cacggtgaga ttgtgagcgg
     9421 acccatcaat cgtggggccc tcaagtgcga gtgcaggctg gaccgggggc tcatctgtgg
     9481 gacgagggat gaatccttcc gacggcccgc catccgtgcc gacgcccacg ctcgcggtcg
     9541 gagcagggcc tgctccgaag aggctgcagc cactgatgag aacgacgatg gcggcagctg
     9601 aaaaaagacc cgagatacgc atatggccga tcgtgccata tgaccctgtg tcacttcgaa
     9661 ccctgtccgc cactgcagca ctaccggaac agcgcaggtg cgaagcagtg tgtacaaatt
     9721 cgcgcagggg tggtgacccg ccgctggctc gtgccgggat gacccgtcca tcaccgatca
     9781 agagatcagc agtgcggcga tctggacggt gttgagggcc gcgctccggc gcaggttctt
     9841 gtcccgcggg tcaatccgtt accgggggtt gacgatccgc acgaccctac ccggcggcgg
     9901 ctcgagtcca tgacccacta cttcgacgcc gtccaccgct tggcgcgcag tggggaggtg
     9961 cttggggaca caggcgaagc cgtcgtctga ccaccgtggg taggcaacga gctcgtgatc
    10021 agtaggcgac ggctcaggcg gcccagcggc gagtctgatc gttaggccgg taacgcgccc
    10081 tgctcccacg atgtcccttg ctgcgctgga aagagccgcc aacgccgagg cgagagcgag
    10141 cccgggcggg tacgagccgt cgggcgggat cacgaccgag gcgcatggat gaccagggat
    10201 ttcgtcgttg agcggatgaa tacccacgcg ggccagaccc tggccggggt gttcggcgga
    10261 ctcatgcggc tcgcaggtga tccgccctat gagttcgtcg gccaaccgag tcagttcgcg
    10321 taggggtagg tcctcaagcg cacctggcag gtagccatcg tgcccgtcgc ccgggaagcg
    10381 cggggccagg tgaaggtgca cgtgcggcgt gtgctggccc ggcgggcccc agttcgcgcg
    10441 gagggtcacc ttggtggcac caagggcaga acgcagaccc gccgccaccc gcttcgtcac
    10501 ccacgccaga tcgacgagct ccgaccagtc gaggccgctc aggtctgcga cgtgccgacg
    10561 cgtggtgacg agcgtgtagc caggattgga ccgcatccct cgcgacgcca agcgagccgt
    10621 cgtggacggc gtggagcaaa cgaccagccc gtcaaacggc gggcagtggg ggcacccgtc
    10681 gcgcctcact cgttctccag gcgagaacgg gttggctgga acgccgtcac atccgacacc
    10741 atgcgtcgct ggctggccgc cgccgccgcc gatcaggaga tcaacagctc ggcgatctgg
    10801 acggtgttga gggcggcgcc cttgcgcagg ttgtcgttgc tgacgaacag cgccaggcct
    10861 cggccaccgt cgaccgactg gtcctcgcgg atccggccga cgtaggacgg gtcggcgccg
    10921 gccgcctgca gcggggtggg cacgtcggcc aactcgacac cgggagcgtt ggccagcaac
    10981 tcgcgagccc gcgccggcgt gatgggccgc tcgaactcgg cgttgatcga caacgaatgg
    11041 ccggtgaaga cggggacccg cacgcaggtc ccggacaccc ggaggtcagg cagatcgagg
    11101 atcttgcgcg actcgttgcg cagcttgcgc tcctcgtcgg tctcctcgct gccgtcatcg
    11161 agcaccgagc cggcgaccgg gacgacgtcg aaggcgatgg gggcgaggta cttcacgggg
    11221 gcgcccaggt cgaccgcgcg tccgtcgtgg gccagcccct cgatgtcccc agactccagc
    11281 gcccggcgga cctgggttgc caactcctga accccggcga gaccggtgcc ggagacggcc
    11341 tggtaggtcg agacgatcag gcgcttgagc ccggcctcgt cggccagcgg cttgagcacc
    11401 ggcatcgcgg ccatcgtcgt gcagttgggg ttggcgatga tccccttcgg agggttcttg
    11461 gccgccgccg ggttgacctc ggagacgacc aacggcacct cggggtccat ccgccaggcc
    11521 gacgagttgt cgatgacggt gacgccggcc gccgcgaacc gcggagcctg cgccttggag
    11581 gtcgtggcac cggcggagaa gatcgcgatg tgcaggcccg tcgggtcggc ggtcgaggcg
    11641 tcctcgacga cgatgtcccg gccctgccag gtgatcgtcg agcccgccga gcgggacgag
    11701 gcgaacagcc gcaactgacc gaccgggaag ttgcgctcgg cgaggatccg caggacgacc
    11761 ccgccgacct ggccggtcgc cccgacgatt ccgacgttga ctgcagcgga cggcatactc
    11821 atcggccggt ccctccgtag acgatggcct cgccctccgc ggagtcgagg tcgaaggcgg
    11881 tgtgggcggc gcggaccgcc gcgtcgagct ggtcggcacg ggtgacgacc gagatgcgga
    11941 tctcggaggt cgagatcatc tcgatgttga tgccggcgtg ggcgagggcc cggaagagct
    12001 tcgcggagat gccggggttg gtgcgcatgc cggcaccgac gagggagagc ttgccgatct
    12061 ggtcgtcgaa ccgcagttcg tcgaagccga tcgccgcgcg cagggactgc agcgtctgaa
    12121 ccgccttggg cccgtcggag gacgggagcg tgaacgagat gtcggtgcgc ccggtggcgg
    12181 ccgccgaaac gttctggacg atcatgtcga tgttgattcc ggcgtcggcg accgcggtga
    12241 agatgtcggc ggccttcccg gcctcgtcgg gcaccccgac gatggtgatc ttggcctcgc
    12301 tgcggtcgtg ggcgacgccg gcgatgatcg gggcttccat ggtgcttcct tcgccgtagg
    12361 ggttctcgag gatccaggtg ccctcgtggc cggaccacga cgagcggacg tggatcgcga
    12421 tgccgtgccg acgggcatat tcgacacagc gcaggtgcag gatcttggac ccgttggcgg
    12481 ccaggtcgag catctcctcg ctggagatga ccgacagttt gcgcgccgtc gggagcaccc
    12541 gcggatcggc ggtgaagacg ccgtcgacat cggtgtagat ctcgcagacg tcggccttga
    12601 gcgcggcggc gagcgccacc gcggtcgtgt ccgaaccacc ccggccaagg gtcgtgatgt
    12661 ccttgctcgt ctggctgacg ccctggaagc cggcgacgat ggcgatgtgc ccggcggcca
    12721 gcgcctcggt gatccgccca ggggtcacgt cgatgatccg cgccttgccg tgctcggcgt
    12781 cggtgatgac accggcttgc gaccccgtga acgaccgggc ctcgctgccg aggtcggcga
    12841 tcgccatggc cagaacggcc atcgagatcc gctcaccggc ggtgaggagc atgtccaact
    12901 cccgagccgg agggttcggg gtgaccttgt tggccaggtc gatgagctcg tcagtggagt
    12961 cgcccatggc agagacgacg acacacacgt cgtggccggc ctgtttggtc tcgacgatcc
    13021 gccgggcgac ccgcttgacg ccctcggcgt cggcgaccga cgacccgccg tacttctgga
    13081 cgatgacgct cactgacctg ctcccggaga cggacctgtg gaccgcccga gtttactcag
    13141 ggcgcgcacg ccaacagccc gccctccgca tcgcgagacg gctgtctcag ctggcggtca
    13201 ggggtgcaga gcgtcgaact cggcgtccgc gacgacgtcc tcgtcggcgt ccagccgcag
    13261 gtgggcgatg atcgattgga gcacccgcaa cgatgcggca gcccgctcgc cccaggtcga
    13321 caggtaggag aactgccacc accacagggc ctcgacgcgt cggccggcgt cgtagtggct
    13381 caggccatga gccaacgcca ggcagatctc ggtcatgtcg ttggacagcg accccttggt
    13441 gagctcggcc tgcgtgaccg ggtcaacgag gtcggcgtag tcgtcgatgc cttcgaagag
    13501 gttggcgagg ttgtcgcgca agggttcgac gtcggcgtct gggccggggt cgggctcgta
    13561 gcgctcgtcg aggacgacgt cctggactgc gccgagtcgt gacccggcca ggagcaactg
    13621 agcggtcgcg agcaacagca aggggatcgc gatgtcgggc gaggacccgg aggccacctc
    13681 ggtcaccgcc gtgacgtagg cccgcgcttg cagcgccgag tcgtcggcaa gcccggcgaa
    13741 ctcggcgaag tcgtcgaact cggagcccgg ctcagcactg gccgacaccg cgtccggctc
    13801 tgcgggcggc atacccgatc ggtcggcgtc aggcatggac ggtgcgccgt ccttcgaaag
    13861 cgcgtcccaa ggtcacctcg tcagcgtatt cgaggtcgcc ccccaccggc aggcccgagg
    13921 cgaggcgagt cacacggatc cccagcggcg cgagcgtgcg ggtgagatag gtggcggtcg
    13981 cttcaccggc gatgttgggg tcggtggcaa tgatgacctc gacgatgccc ccgtcggcga
    14041 ggcgggtcat caactcgcgg atccgcagat cttccgggcc gatgccgtcc atcgggctga
    14101 tcgcgccgcc gagcacgtgg tagcgcccgc ggaactcccg ggtgcgctcg atggcggcga
    14161 cgtccttggg ctcctcgacg acgcagatcg ccgtctggtc gcgccgggag tcgccacaga
    14221 tccggcagag ggtcgcctcg gcgacgttgc cgcacaactc gcagaagcgg atcttgtcct
    14281 tgacctcggc cagcacctgg acgaggcgcg ccacatcggc cggatcggcc tggacgaggt
    14341 gaaaagcgat gcgctgagcg ctcttcgggc cgattccggg cagccgtccg agctcgtcga
    14401 tgaggtcctg gacgatgcct tcgtacaccg ggccagacta cgtgcgaccg gcgacaatca
    14461 cgcgctcccg cgccaggaca cgagggcgtc ggccagcggc tcgaccaggt cacgcccgcg
    14521 cagggcagtg cgccaacgca gcggtatgcc gccctccccc gccacgatcc ccgccagccc
    14581 agcggcgatg cacgcggtcg tgtccgtgtc ctcgcccagc gagatcgccg ccttgacgac
    14641 ccgttcgtag tccagcggat cgtcggccag cagcagcgcg accgccgcac gcatcgactc
    14701 cacgacgtac ccaccaccgc ggatttcgta tgccgcgccg tcggggaaga cgtgcgtcac
    14761 cagttcgtcg tgctcggggc tgccagccgg gtagacctcg tcgaaagcgg ctcgcgcggc
    14821 cggccaggcc tgggcgggcg gcataccctg caggaggcgc cgggcgagca ggcagtagag
    14881 cacgcagcag acctggctgc gcagatgacc gtgggtgacc gccgactgcg ccatcgcatc
    14941 ggccgccagg tcagcgtcgg agccgcggtg ccagagcgcg aggggcagga cccgcatgag
    15001 cgagccgttg ccgttggccc gcgagtcggt gggcccggac gacgcggcgg gcaccccggc
    15061 acgcagggat ctgatggcga tcccggtctg gatgccgatg tcgaagacgt ggcggtcgac
    15121 ggcataccgg ccctcgtcgt cccacgcgac cagtccgcgg ccgacgtcgt ccacgtcgag
    15181 gtgacccacc cgcaggagcg attcgagcag gacgagggcc tgggcaccgt cgtccgacca
    15241 ggtgccgggg gcgatcccgg catgggcccg ggcgaagccg gcgggcggga ccatctcgat
    15301 ctcgtcggtg gcagggatct gcgtggctgg gtggaattcg tagggcactc cgagcgcatc
    15361 accgacgaga agcccgaaga tcccgccgcg caggcggtcg ccagtggtca tgccaggcat
    15421 tgtccctcga tcccctaaca ccccagtggt cccgtcggtc gggtcgatgg acccgggcga
    15481 ggccgccgcc agtcgctcag gaggggtcga gggtggggac ctcgttgtag gtgtcggcgc
    15541 gctcctgacc gctgaggagc gcgatggcgt ccatgacgtc gtcggtgagc tcgcgtcgag
    15601 ctcgcccggc cggcatcccc gcgtaggtct cgggatggat cggcggcccg aaggccaccg
    15661 agaccttggc cagccgcggc cacctggacc ccaccggctg gatctgatcg gtgccgcgca
    15721 ggccgacggg cacgacgggt acgccagcgg tcagcgccaa ccagccgaca ccggtgcgcc
    15781 cgcgatagag ccgaccgtca cgcgagcggg tgccctcggg atagatcccg aaggcgcctc
    15841 cagcctgcag gacctcaagc gcgaggtcga gggactcctg agccgctcgc gaggagttcc
    15901 ggtcgacggg aatggcgccg acgctgctga agaacccgcg gctgagcaga ccccgaggcc
    15961 ccgagccggt gaagtagtcg gacttggcca ggaaactcac cttgcgcggc gccaccagcg
    16021 ggatggcgaa ggagtcgatg aacgacaggt ggttgctggc caggatgacc ccgcccttgg
    16081 gggggacgtt ctcgatgccg gtgatcgtcg gccgatacag cagccgcgac accggttgca
    16141 gcagcgtcgc accgactcgg tagaacatgg gcccaaccgt agtggcgggc acgctcgcca
    16201 cgcagccccc aggccaatcg atggaccgcg acctcaggtc ggcgtgtcgc cgagcaccgt
    16261 gccaccgagc atccgctcga tgacggcctg cccgatctcg gcgccctctt gcacgacctc
    16321 gtcgtccagg ctcgctgcgc ccaggtcact ctgcggcgac gtcgcgcgcg gagccggcgc
    16381 ggggtcggag cgatgatggc gctcgtccat ggtgcgccgg gtggcctcct tggcgcgcgc
    16441 gagctc
//
LOCUS       DAOMZY010000024        16129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_3252_length_16129_cov_6.067500, whole genome shotgun sequence.
ACCESSION   DAOMZY010000024 DAOMZY010000000
VERSION     DAOMZY010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 16129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16129
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3252_length_16129_cov_6.067500"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..1015)
                     /locus_tag="PLK64_02645"
     CDS             complement(44..1015)
                     /locus_tag="PLK64_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV78669.1"
                     /translation="MIAAEATPAEPTPSGTPAELIQARGLTKRFGDFTAVDGIDVTVT
                     AGESFGFLGPNGAGKSSTMRMIAAVSPVTSGTLRIFGLDPATRGPDIRAALGVVPQKD
                     TLDEELTVEENLWIYGRYFGLSWAECRRRATELLEFAQLTERAGDRVEPLSGGMKRRL
                     TIARSLINDPRILLLDEPTTGLDPQARHVLWDRLFRLKRQGVTLVVTTHYMDEAEQLC
                     DRLVVMDHGQIVAEGSPRALIQTHATREVLELRFTDDDISVHVPSLIGIGDRVEVLPD
                     RLLVYADDGDAALAAAHARGIQPLSALVRRSSLEDVFLHLTGRTLVD"
     gene            complement(1012..1689)
                     /locus_tag="PLK64_02650"
     CDS             complement(1012..1689)
                     /locus_tag="PLK64_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPV78670.1"
                     /translation="MSRVLIVDDDPQLLRALRINLVARHHDVQVATTGADALQLAAIH
                     LPDLVLLDLGLPDLDGVDVIHGLRGWTDVPIIVLSGRSGGPDKVAALDAGADDYVTKP
                     FGVEELLARMRAVARRGSGAEGGDPVVRFGDLSIDLVNRTVTGPTGAGPSGAGAVSAG
                     RGIPQSAGKPAPLCLPTATQTRARPRSSATPGDRARHGLPLPAMTRSGQPKPSDGLSP
                     LVVTLGG"
     gene            complement(1686..4226)
                     /locus_tag="PLK64_02655"
     CDS             complement(1686..4226)
                     /locus_tag="PLK64_02655"
                     /inference="COORDINATES: protein motif:HMM:NF014728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HPV78671.1"
                     /translation="MGPEPRSGLVSEWAPKEASGMPRGRLRVYVGVAPGVGATYAMLA
                     EAARRRARGADVVIGVADTHGRAPLEALMVGSERREPGALDVPALLERRPRIVAVDNL
                     GAIDATTGRARWESVEQLIEAGINVLATTEVRAVASLAEVVAAVTQGPPPPPVPDRIL
                     LGADQVELIDMAPEALRRRLAHGGLRSAGQVDATTASLYSVETLSRLRELGFSWMTGL
                     LAAARPESADPGEVGERILVAVPSGPDAVPLLHRAARLANRAPASELAAVHVVSGRSL
                     PGSADLDLVALRRVAESVGAWFHHVVGEDVPQALAEVAMAEHATALVVGGGAPAEGLR
                     RVWGGRHTGALAVRVLDQALGVDVHVVPTASTRSHTRSKPERIPLSRVRVASGTALAV
                     VLPAGLTPLLLMAGDRVGLAGHSLAYLLTVVVVALVGGLWPALVCAVAASTLLNYYFI
                     PPTHTFQVADLHNTVTLVGFLVVAGLVGAIVHRAATMTLAAARASARARTLAAVAEGT
                     IRGEEALPALLEQLRSSFGMTSVSLLAGSDDSHDQSDEPWSVLRSVGPDAPTDPRQSD
                     VQVSAGPGLTLALAGRALVAEERATLRAFAAQVRVVHERDQLSREAAVAERLEATGRL
                     RDALLAAVGHDLRTPLAAATAAVTSLRSADVAWSPSERDELLATAEESLHRLTALVAD
                     LLDLSRLRSGTLTVRAETVWLDDVLGPALDELGVVPGGVQIDIPEGLPPVVADAALLT
                     RALVNVLANALRHSPAGERVIITASPSLGRVEIRVVDRGPGIPAADHARVFQPFQRLG
                     DSDNRTGLGLGLALSRGLVEAMSGALTPEDTPGGGLTMVIGLPEGVTA"
     gene            4295..6289
                     /locus_tag="PLK64_02660"
     CDS             4295..6289
                     /locus_tag="PLK64_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HPV78672.1"
                     /translation="MTTTVTLAKRVLVGAPMRSENLGETLLPKRVALPIFSSDALSSV
                     AYATEAILKVLTVGGLAYLYLTPWVALAVALLMTIVVVSYRQVIHAYPDGGGSYEVVR
                     QNLGRGAGMVVAASLMVDYVMTVAVSVAAGVDNVISAVPSLNSQRVLLAVGCVAVLTL
                     VNLRGVRESGRAFAVPTYLFIVGIIAMVGIGLFQTAVGHAPVAVSATYQIREEAFGLT
                     GIALLMLGLRAFSSGCTALTGVEAISNGVPAFKAPKARNAARTLGLMGAIAIVMFVGI
                     TILALVAQVRVAEDTCALVGFAGDCASDPQLTVIAQLAGSVFGADSPAFYFVQGTTAL
                     VLVLAANTAYNGFPMLTSVLARHDLVPRQLRNRGDRLSFSNGNIALAAAAVALLVVYG
                     ADVDQLLHLYVLGVFTSFTLSQIGMVKHWNQQLSEPTTLPSDFARIRLARVVNGVGAV
                     LTAVVLVIVLVTKFTSGAYLVVIAVPLLCLLMREVSRHYARVRRLLASQGNPTRLPSR
                     VHAVVLASTLHEPTLRAVAFARATRPSTLTALTVQVDQDETEALCAAWADAEIPVTLT
                     VVGSPFRDITTPIVDYLQRIRLESPRDLVAVYLPEYVVSHWWEELLHNQSALRLKLRL
                     RLMGNVMLTSVPYVLDGADDPKNTDDDGEPTRPRVAPITP"
     gene            6360..7655
                     /locus_tag="PLK64_02665"
     CDS             6360..7655
                     /locus_tag="PLK64_02665"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HPV78673.1"
                     /translation="MSRTAKLELDGKSYELPIVEGTEKELAIDISSLRAQTGYITLDD
                     GYGNTGSCSSAITFIDGDKGILRYRGIPIEEIAGKSSFVETAWLVMFGHLPTVDDRER
                     FSKLLTENSRLDESMRKHFEGFPPNGHPMAILSAMINALSTHEPVIIEDERDIEEAAA
                     RLMSKVRTIAAAAYKASVGEPIVYPRYDMKYVENFLHMMFSIPNRDYEPTPEVSRALN
                     LFLSLHADHEQNCSTSTVRMVASSQANMFASCSAGVCALWGPLHGGANVAVIEMLEMI
                     RESGMPVKEYVARVKDKQSGIKLMGFGHRVYRNFDPRSIILKQAADDMLAKMEVHDPL
                     LDIAVELADVALSDPYFVERKLYPNVDFYSGTILRAIGIPLNMFTVMFAIGRMPGWIA
                     NWKEIHDDPKGRIYRPRQVYVGHTGEHWMPREERHYDED"
     gene            complement(7683..7904)
                     /locus_tag="PLK64_02670"
     CDS             complement(7683..7904)
                     /locus_tag="PLK64_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3046 domain-containing protein"
                     /protein_id="HPV78674.1"
                     /translation="MRYSELATMVAQEFGQGYAVSLLRSHVLVALGNRTGDAALADGI
                     PPRQVWAAICDDFDIPAERRFGQDRPKRR"
     gene            7976..12877
                     /locus_tag="PLK64_02675"
     CDS             7976..12877
                     /locus_tag="PLK64_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPV78675.1"
                     /translation="MGESVLDRFSPATADWFRGSFLAPTPAQEGAWDAISSGRHAVVV
                     APTGSGKTLAAFLWALDRLSVAPVPEDRARRCRVLYISPLKALAVDVERNLRSPLVGI
                     GHAAARLGMPLPQVRVAVRSGDTPAADRRGFVRDPSDILITTPESLFLMLTSAARESL
                     VGVETVILDEVHAVAGTKRGAHLALTLERLDALLERPAQRIGLSATVRPVEEVARFLA
                     GGRPVDIVAPASTKQWDLQVVVPVPDLGAIGSPTGDLSGPAAGDQARASIWPHVEERI
                     VDLVAAHRSTLVFANSRRLAERLTTRLNEIWQERTEESLDNAETPVGSGSAGTTGMAG
                     TTGMAGTTGLGAHGIPAGAAWGGAAGGMPAQIMAQSGAARGAPALLARAHHGSVSKEQ
                     RALIEEDLKAGRLPAVVATSSLELGIDMGAVDLVVQVESPPSVASGLQRVGRAGHQVG
                     AVSRGVLFPKFRGDLVQTAVVVERMRTGSIESLRVPGNPLDVLSQHIVAMCAMDDWSV
                     EELEALVRRAAPFAGLSRLVLESVLDMLSGRYPSDEFAELRPRIVWDRVAGTLHGRRG
                     AQHLAVTSGGTIPDRGLYAVFLASGDGPGRRVGELDEEMVYESRVGDTFTLGTSTWRI
                     EDITHDRVLVTPAPGQPGRLPFWKGDTLGRPVELGAAVGAFVREVGGLATAAARKRVG
                     AAGLDEWATDNLLGYLTEQREAIPQLPTDRMIVVERFRDELGDWRVVIHSPFGSPVHA
                     PWALAVSARMRERFGVDVQAMHGDDGIVLRLPDLDLGGEVGVNADAGSATIGAELTQE
                     LLDLILLDPDEVADLVTAQVGGSALFASRFRECAARALLLPRRRPDRRQALWQQRQRA
                     SQLLEVASRYPAFPIVLEAVRECVQDVFDVPGLVGLMRAIRSREVGALAVQTRVPSPF
                     ARSLLFGYVAQYLYEGDSPLAERRAAALALDPTLLAELLGTGDGLSLRDLLDPAAVAT
                     TEAELQRLVPERAARDAEDLADLVRVLGPLGVDDLVARSVTSDARVVGAWLVELESAR
                     RVIRIRIGGQERWAAVEDAGRLRDALGVSLPVGVPEVFLAPVADPLGDLVARFARSHG
                     PFGVHAVAAWLGLGPAVVRDALRRLVAAGRLVEGELRPQGSADGGLDYCDAGVLRTLR
                     RRSLAALRAEVEPVGPVDYARFLPTWQGVGGTLHGPEGLLRAVEQLSGALLPASAVES
                     LVLPSRVAAYQPAWLDELLAAGEVLWQGHGALPGDDGWVSLHVADLAPTTLTPATALA
                     DLGDIHAAILECLGNGGAYFFRTLIDAVSAAVGPSPDEAVRTALWELVWGGQISGDTL
                     APLRALLGGGRTAHRRRATPGPRGRYAARGTSLGLGTSRLGWGAVGGEGGASAGGAVG
                     VGSGVGGAARLGGALGRAGRFAPTAADVVGRWALLPGRESDPTVTAYTRAEVLLDRYG
                     VLTRGSVGAEDVPGGFAGVYRVLAAAEESGRVRRGYFVEGLGAAQFGSTGAVDRLRAG
                     VRPLGESARAILPALVLAACDPANPYGAALPWPGREPVEGGGRGHQPARKAGALVVLV
                     DGELVLYVERGGRSLLTWSEDPAALQAAAGALALAVREGALGKLTVERTDGASVLGSD
                     HALAAALAQAGFHATPRGLRLRR"
     gene            12920..13831
                     /locus_tag="PLK64_02680"
     CDS             12920..13831
                     /locus_tag="PLK64_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HPV78676.1"
                     /translation="MPEGDTVWRTAQRLNEALAGDPLVHADLRWPSLALADLRGAVTL
                     EIRARGKHLLHRLDNGLTLHSHLRMEGQWRIESTATLSVRRLADDDLRAVLGTTRWTA
                     LGLRLGMLDLVTTSDEASLVGHLGPDVLGSDWDPVIATSNLAASASMIGAALLDQRNL
                     AGVGTIYAAESLFLQRLSPWALATELPTTAVAALVERAHQLLDIGRRNPLQSTTGRTR
                     RGETTFVHGRSGRPCLRCGTIIRLGMVGTAPQERAMFYCPTCQGVRSVQDVREFQADR
                     SLPGDRSVQGVRTDGRRPPRPSPSRGR"
     gene            13842..14495
                     /locus_tag="PLK64_02685"
     CDS             13842..14495
                     /locus_tag="PLK64_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPV78677.1"
                     /translation="MTDEHLGGPTVVAAGVSKTISDTTLLAPTDLRAESGTCVVVRGR
                     NGIGKTTLLRILAGTLEPTTGTASIDGVRLDERDAATRRRVAALLGAPTAYRDLTLAD
                     HLTLIDATWGRDVAGCAQRVADGLDALGIGDLAHRFPHELSSGQSQLFRLAMVFFRPG
                     DLLVLDEPEQRLDTTKRALVGDLLIHRRQAGDTLLVACHDPVLTERIADVVLDLPEP"
     gene            14492..>15145
                     /locus_tag="PLK64_02690"
     CDS             14492..>15145
                     /locus_tag="PLK64_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78678.1"
                     /translation="MNDRERLEDVEHVLHGDKGSGAAKNMGYAVYVTVLLASTYGFTV
                     ARAVFATSGPAWIDDHVLTLPTAVIGVGLFVASLALARWAGRTRGPVVPPLPWTDLVA
                     SSDVDRARSLRRWWTYAFVGGLVGGAILGAVIGGGVWASGAGSAGWFVGAVPIGAAAG
                     WLLARMWLEGQVAPDTSGTATLARVVRPAAGSLRQLRLDDLRSHGVRSTQMGGGVLAG
                     "
     assembly_gap    15146..15155
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15156..15961
                     /locus_tag="PLK64_02695"
     CDS             <15156..15961
                     /locus_tag="PLK64_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78679.1"
                     /translation="DLRAVRLEVASPVTRGRRLRLQAAGPRAVVVRRDILGLRRAPGA
                     FWTGAVLVVIGWAGVVWALTDPRVPVALTCLAMAACYLGMGAWAEGLRLLGDNAGTPP
                     MLGLRFRTEAIAHLVVPVALFLMTTVGVSAIGLMAMNNVGVDGAGLSSAGPNGAVPIL
                     AVLGWCLLIGTLLAGAQLMAAFRGAPPVMAFLPEYGPPMMIYWYARPLVLASVSGGLI
                     VSHATAGAAASTAAGSGMVTGMLIALVTAIGAMAWGLRSVKTLEEAHRI"
BASE COUNT         2241 a         5779 c         5596 g         2503 t
ORIGIN      
        1 ccgtgcggct gggttcgctc gtcgcgcgcg ggtggatggt ccatcagtcc accaacgtgc
       61 gtccggtgag gtgcaggaac acgtcctcca gcgacgagcg gcgcacaaga gctgacagcg
      121 gctggatccc gcgggcgtgc gcggcggcga gcgcggcgtc gccgtcgtcg gcatagacga
      181 gcagtcggtc cggcagcacc tcgacccggt cgccgatgcc gatcagcgac gggacgtgga
      241 cggagatgtc gtcgtcggtg aagcggagct cgaggacctc gcgggtcgca tgggtctgaa
      301 tgagcgcccg aggtgaaccc tcggcgacga tttggccgtg gtccatgacg accagtcggt
      361 cgcacaattg ctcggcctcg tccatgtagt gcgtcgtgac gacgagcgtg acgccttggc
      421 gcttgagccg gaacagtcga tcccagagga cgtggcgggc ctgtgggtcc aaaccggtgg
      481 tcggctcgtc gagcagcagg atccgcgggt cgttgatgag cgaccgggcg atggtgaggc
      541 gccgcttcat cccacccgac aacggctcga cccggtcgcc ggcccgctca gtgagctggg
      601 cgaactccag caactccgtt gcgcggcgcc gacattcggc ccacgagagg ccgaagtagc
      661 ggccgtagat ccagaggttc tcctcgacgg tcaactcttc gtcgagcgtg tccttctgcg
      721 ggacgacacc cagcgcggct cggatgtccg ggccccgggt cgccgggtcg aggccgaaga
      781 tccgcagggt gccgctggtc acgggtgaca cggccgcgat catccgcatc gtcgaggact
      841 tgcccgcgcc gttcggcccg aggaagccga aggactcccc cgcggtcacc gtgacgtcga
      901 tcccgtcgac ggccgtgaag tcaccgaacc gcttggtgag gcctcgggcc tggatgagct
      961 ccgcaggggt ccccgacggt gttggctcag cgggtgttgc ctcggccgcg atcatccgcc
     1021 cagggtaacc accagcggtg acagcccgtc actgggtttt ggctgccccg accgggtcat
     1081 ggccggaagc ggtagcccat gccgggctcg gtcaccaggt gtcgcggacg atcggggtcg
     1141 ggctcgagtt tgcgtcgcag ttgggagaca tagaggcgca ggtttgccgg ctgattgagg
     1201 tatgccgcgc cccgcgctca ccgccccagc gcccgacggc ccagcgcccg tcggccccgt
     1261 cacggtccgg ttgaccaggt cgatcgacag gtcgccgaac ctgacgacgg ggtcaccgcc
     1321 ctcagcaccg gacccgcgac gggccaccgc ccgcatccgg gccagcaact cctcgacgcc
     1381 gaacggcttg gtgacatagt cgtcggcccc cgcgtcgagt gcggccacct tgtccggccc
     1441 cccggaccgg cccgacagca cgatgatcgg cacgtcggtc cagccccgca ggccgtggat
     1501 gacatcgacc ccgtccagat cggggagccc gaggtcgagc agcacgaggt caggcaggtg
     1561 gatcgccgcc aattgcagcg catcggcgcc ggtggtggcg acctggacgt cgtggtgtcg
     1621 agcgaccagg ttgatccgca aggcacgaag caactgcggg tcgtcgtcga cgatgagaac
     1681 ccgactcatg ccgtgacccc ttccggtagg ccgatgacca tcgtcagccc gccgcctgga
     1741 gtgtcctcag gggtgagcgc gccggacatc gcttcgacca aacctcggga aagggccaat
     1801 cccagaccga ggccggtgcg attgtcgctg tcgcccagtc gctggaaggg ctggaagacc
     1861 cgcgcatggt cggccgccgg gatccccggg ccgcggtcga ccacccggat ctccacccgt
     1921 cccagcgacg ggctggcagt gatgatgacg cgctcgccgg cggggctgtg ccgaagggcg
     1981 ttcgccagca cattgacgag agcgcgggtg agcagggccg cgtcggccac gaccggtggc
     2041 aggccctcgg ggatgtcgat ctgcactcca ccaggcacca ccccgagctc gtccagcgcc
     2101 gggcctagga cgtcgtcgag ccacaccgtc tcagcgcgca cggtgagggt gcctgatcgc
     2161 agccgggaca ggtccaatag gtcggcgacc aacgcggtca accggtgcag cgactcctcc
     2221 gccgtcgcaa gcaactcgtc ccgctcggag ggcgaccagg ccacatcagc gctgcgcagg
     2281 ctggtcaccg ccgcggtcgc cgccgccagc ggggttcgca gatcgtgccc gacggctgcc
     2341 agcagggcgt cacgcagccg tccggtggcc tcgagccgct cggccaccgc agcctcgcgg
     2401 gacagttggt cacgctcgtg caccacccgg acctgcgccg cgaaagcccg cagggtcgcc
     2461 cgctcctccg cgacgagagc gcgacccgcg agggccaggg tgagccctgg cccggcggac
     2521 acctgcacat cgctctgccg cggatcggtc ggcgcgtccg gccccaccga ccggagcacc
     2581 gaccacggct cgtcactctg gtcgtggctg tcgtcggagc cggcgagcag gctcaccgac
     2641 gtcatcccga agctgctgcg caattgctcg agtagggccg ggagggcctc ctcgccgcgg
     2701 atcgtgccct cggccacggc cgcgagggtc cgcgcgcgcg ccgacgcccg cgccgcagcc
     2761 aaggtcatcg ttgcggcccg atggacgatc gccccaacga gcccagccac cacgaggaat
     2821 ccgaccaggg tcaccgtgtt gtgcaggtcg gccacttgga acgtatgggt cggtgggatg
     2881 aagtagtagt tgagcagggt cgacgccgcg accgcgcaga ccagggccgg ccagaggccg
     2941 ccgacgagcg cgaccacgac caccgtcagc agataggcca agctgtggcc ggccaggccg
     3001 acccggtccc ccgccatcag cagcaggggg gtcaagccgg ccgggaggac gaccgccagc
     3061 gcggtcccac tggccacccg gacccgagac agcgggatcc gttccggctt ggaccgggtg
     3121 tgtgagcggg tcgacgcggt cggcacgaca tggacgtcga ccccgagcgc ctgatccagc
     3181 acccgcacag ccaacgcccc ggtatgccgc ccgccccaga cccgccgtag gccctcagcg
     3241 ggcgcgccgc ctcccacgac caaagcggtc gcgtgctcag ccatggcgac ttcggcgagc
     3301 gcttgcggga cgtcctcgcc gacgacgtgg tggaaccagg cgccgacgct ctcggccacc
     3361 cgacgtaggg cgaccaggtc gaggtcggcc gaccccggca acgaccgccc gctgacgacg
     3421 tggacggccg ccaactccga cgcgggcgcc cgattggcca gccgggcggc tcgatgaagc
     3481 aagggcaccg cgtccgggcc agacggcacc gcgacgagga tgcgttcgcc gacctcgcca
     3541 gggtcggccg actccgggcg cgccgcggcg agcaggcccg tcatccagct gaacccgagc
     3601 tcgcgcaggc ggctcaacgt ctcgaccgag tagagcgacg ccgtggtggc atcgacctga
     3661 cccgccgagc gcagcccccc atgcgccagt cgcctccgca acgcctccgg tgccatgtcg
     3721 atcagctcga cctggtcggc gccgagcagg atccggtccg gcaccggtgg gggcggcggc
     3781 ccctgggtga ccgcggcgac gacttcggcc agcgaggcca cagcacgtac ctcggtcgtg
     3841 gcaaggacat tgatccctgc ctcgatcaac tgctcgaccg actcccagcg ggcgcgccct
     3901 gtcgtcgcgt cgatcgcgcc caggttgtcg acggcgacga tccgaggccg tcgctccagc
     3961 agcgcaggga cgtccaaggc acccggctca cgtcgctcgg acccgaccat gagggcctcc
     4021 aggggcgccc ggccatgcgt gtcggcgacg ccgatgacga cgtccgcccc tcgggcacgg
     4081 cgacgggccg cctcggcgag catggcgtag gtcgccccga ccccgggcgc gactccgaca
     4141 tacacgcgca accggccacg cggcataccg gaagcctcct tcggtgccca ctctgacacg
     4201 agcccggacc gtggttcggg acccatgcag gatccttgca acaagcagca ggttccatgc
     4261 acgacgctca cggccgtcga cctagcgtcg aaacgtgaca acaacggtga ccctggccaa
     4321 gcgggtcctc gtcggggctc cgatgcgcag cgaaaatctt ggcgagacgc tgctccccaa
     4381 acgggtcgcc ctgcccatct tctcctccga cgccctctcc tcggtggctt acgccaccga
     4441 ggcgatcctc aaggtgctca cggtcggggg gttggcctac ctctatctca ccccgtgggt
     4501 cgccttggcg gtcgccctcc tgatgacgat cgtcgtcgtg tcctaccggc aggtgatcca
     4561 cgcctatccg gacggcggcg gctcctatga agtcgtccgg cagaacctcg gccgcggggc
     4621 cggcatggtc gtcgccgcgt cgctgatggt cgactacgtc atgactgtgg ccgtctcggt
     4681 ggcggccggc gtcgacaacg tcatctcggc cgtcccgtcg ctcaactccc agcgggtcct
     4741 gctcgccgtg gggtgcgtcg ccgtgctgac cctggtcaac ctgcgcgggg tgcgcgagtc
     4801 gggtcgggcg ttcgccgtcc ccacgtatct gttcatcgtc gggatcatcg ccatggtcgg
     4861 gatcgggctg ttccagaccg cggtcggcca cgcaccagtg gccgtcagcg ccacctatca
     4921 gatccgcgag gaggccttcg gcctcaccgg gatcgcccta ctcatgctcg gattgcgcgc
     4981 gttttcctcc gggtgcacgg ccctgaccgg cgtcgaagcc atcagcaacg gggtgcccgc
     5041 cttcaaagcg cccaaggcgc gcaacgcggc gcggacgctg gggctcatgg gagccatcgc
     5101 gatcgtcatg ttcgtcggca tcacgatcct ggccctcgtg gctcaggtcc gggtcgccga
     5161 ggacacctgt gcgctggtgg gattcgccgg cgattgcgcc tccgaccctc aactgaccgt
     5221 catcgcccaa ctcgccggat cggtcttcgg cgccgacagc ccggccttct acttcgtgca
     5281 ggggacgaca gccctcgtcc tcgtcctggc cgcgaacacg gcatacaacg ggttcccgat
     5341 gctcacctcg gtgctggcgc ggcacgatct cgtgccccgg caactgcgca accgcggcga
     5401 ccgactgtcc ttcagcaacg gcaacatcgc cctggccgca gccgccgttg ctctgctcgt
     5461 cgtgtacggg gccgatgtcg accaactcct gcacctctac gtcctgggcg tgttcacctc
     5521 gttcacgctc agccagatcg gcatggtgaa gcactggaac cagcagctga gcgagccgac
     5581 taccctcccc agcgacttcg cacggatccg tctggcgagg gtcgtcaatg gggtcggtgc
     5641 ggtgctcacc gctgtggtgc tcgtcatcgt gctagtcacc aagttcacga gcggggccta
     5701 cctcgtggtg atcgcggtgc cgctgctgtg tctgctcatg cgcgaggtgt cccgccacta
     5761 cgcacgggtg cgccgactgc tggcatcgca gggcaacccg acccggttgc cgagccgggt
     5821 gcatgccgtc gtcttggcct cgaccctgca cgagccgacc ttgcgtgcgg tggccttcgc
     5881 ccgcgcgacc cggccttcga ccctcaccgc ccttacggtg caggtcgacc aggacgagac
     5941 cgaagctctg tgcgccgcct gggccgacgc cgagatcccc gtcacgctca cggtcgtcgg
     6001 ctccccgttc cgcgacatca ccacgccgat cgtcgactac ctccagcgga tccgcctgga
     6061 gagcccgcgg gacctcgtcg ccgtctacct gccggaatat gtcgtctccc actggtggga
     6121 ggagttgctg cacaaccaga gcgccctgcg gctcaagttg cggctgcggt tgatgggaaa
     6181 cgtcatgctg accagcgtgc cttacgtgct ggacggcgcg gatgacccca agaacaccga
     6241 cgacgacggc gagccaaccc ggccgagggt ggcgccgatc actccctgac cggtctaggt
     6301 ggcgccgggt cctgactacg atgcaggcaa cccaaaagac ggcatgtgga ggcgcaccga
     6361 tgtcccgtac ggccaagctc gaactcgacg gtaagtccta cgagttgccc atcgtcgaag
     6421 gcacggagaa ggagctggcg atcgacatca gctcactgcg cgcccagacc ggctacatca
     6481 ccctcgacga cggctacggc aacaccggct cgtgctcgtc ggccatcacc ttcatcgacg
     6541 gcgacaaggg catcctgcgc taccgcggca tccccatcga ggagatcgcc gggaagtcgt
     6601 cgttcgtcga gaccgcctgg ctggtcatgt tcggccacct gcccaccgtc gatgaccgtg
     6661 agcggttctc caagctcctc accgagaaca gccgcctcga cgagagcatg cgcaagcact
     6721 tcgaggggtt cccccccaac ggccacccga tggccatcct ttcggcgatg atcaacgccc
     6781 tctcgaccca cgagccggtc atcatcgagg acgagcgcga catcgaagag gcggccgcgc
     6841 ggctcatgtc caaggtgcgg acgatcgccg cggcggcata caaggcctcc gttggcgagc
     6901 cgatcgtcta cccgcgctac gacatgaagt acgtcgagaa cttcctgcac atgatgttct
     6961 cgatccccaa ccgcgactac gagccgaccc ccgaggtgtc tcgggcgctc aacctcttcc
     7021 tctccctgca cgccgaccac gagcagaact gctcgacctc gacggtgcgc atggtcgcct
     7081 ccagccaggc caacatgttc gcctcgtgct cggccggcgt ctgtgccctc tgggggccgc
     7141 tgcacggtgg cgccaacgtc gcggtcatcg agatgctcga gatgatccgc gagtcgggga
     7201 tgcccgtgaa ggagtacgtc gcccgggtca aggacaagca gtccgggatc aagctcatgg
     7261 gcttcggtca ccgggtctac cgcaacttcg acccgcgctc gatcatcctc aagcaggcgg
     7321 ccgacgacat gctcgccaag atggaggtcc acgacccgct gctggacatc gcggtcgagc
     7381 tggccgacgt ggcgttgtct gacccctact tcgtcgagcg caagctctac cccaacgtcg
     7441 acttctactc cggcacgatc ctgcgcgcca tcggcatccc gctgaacatg ttcaccgtga
     7501 tgttcgccat cggccggatg cccggttgga tcgccaactg gaaagaaatc cacgacgacc
     7561 ccaagggccg catctaccgc ccgcgccagg tctacgtcgg ccacaccggc gagcactgga
     7621 tgccgcgcga agagcgccac tacgacgagg actgaccctt cgggcacgtc cccccggcac
     7681 cctcagcggc gcttcggtcg atcctgaccg aagcgccgct ccgctgggat gtcgaagtcg
     7741 tcacagatcg cggcccacac ctgacgcgga ggtatgccgt ccgccaacgc cgcgtcgccg
     7801 gtgcggttcc cgagggcgac caggacgtgc gagcgaagga ggctcacggc atacccctgg
     7861 ccgaactcct gcgccaccat ggtggccaat tcgctgtacc gcaccgggtg agtgtgccac
     7921 gtggaccgcg tgaccggcgc ggctgtcggt gcgtcgtggc agggtggacc ccgacatggg
     7981 cgagagcgtg ctggaccggt tctcccccgc gaccgctgac tggttccggg ggtccttcct
     8041 cgcgcccacg cccgcgcaag aaggcgcctg ggacgccatc tcctccggcc gccacgccgt
     8101 cgtcgtcgcg cccaccgggt cgggcaagac gttggcggca ttcctctggg cgctcgaccg
     8161 gttgtcggtg gcccccgtcc ccgaagaccg ggcccgccgc tgccgggtcc tctacatctc
     8221 gccgctcaag gcgctggcgg tcgacgtcga gcgcaacctg cggtccccgc tcgtcggcat
     8281 cggccacgcc gccgcccgat tgggtatgcc gctcccccag gtccgcgtgg ccgtccggtc
     8341 cggcgacacc ccggcagccg accggcgcgg cttcgtccgc gacccgtccg acatcctcat
     8401 caccactccc gagtcactgt tcctcatgct cacctccgcg gcccgcgagt cgctggtcgg
     8461 cgttgagacg gtcatcctcg atgaggtcca cgccgtcgcg ggcaccaaac ggggggcgca
     8521 cctggcgctg acccttgagc ggctcgacgc cttgctggag cggcccgcgc agcggatcgg
     8581 gctgtccgcg accgtgcggc ccgtcgagga ggtcgcacgt ttcctcgcgg ggggtcggcc
     8641 ggtcgacatc gtggcgccgg cgtccaccaa gcagtgggac ctgcaggtcg ttgtgccagt
     8701 gcctgatctg ggtgccatcg ggtcgcccac gggtgacctg tctggtccgg cggccggcga
     8761 ccaggcgcgg gcgtcgatct ggccgcacgt cgaggagcgc atcgtcgacc tcgttgccgc
     8821 gcaccgctcg acgcttgtct ttgccaactc gcgacggctg gccgagcggc tgacgacgcg
     8881 gctcaacgag atctggcagg agcgcaccga ggaatcactc gacaatgccg agacgccagt
     8941 tggctcgggg tcggcgggta cgacgggtat ggctggtacg acgggtatgg ctggtacgac
     9001 gggcctgggg gcgcacggca tacctgctgg tgcggcctgg ggaggtgcgg ccggtgggat
     9061 gccggcgcag atcatggctc agtcgggggc cgcgcgaggg gcgcctgcgc tgctcgctcg
     9121 cgcccaccat ggatctgtga gcaaggagca gcgggcgctc atcgaggagg acctcaaggc
     9181 cggacggcta cccgcggtcg tcgccacgag cagcctcgag ttgggcatcg acatgggcgc
     9241 cgtcgacctg gtcgtccagg tcgaatcgcc cccctccgtg gccagcggcc tgcagcgggt
     9301 cgggcgggcc ggtcaccagg tgggggcggt gtctcggggc gtgctgttcc cgaagttccg
     9361 cggcgacctc gtgcagaccg ccgtcgtcgt cgagcggatg cgcaccgggt cgatcgagtc
     9421 gctgcgcgtc cccggcaacc cgctcgacgt gctctcccaa cacatcgtcg cgatgtgcgc
     9481 catggacgac tggtcggtcg aggagttgga ggccctggtc cggcgggcgg cacccttcgc
     9541 ggggctgtcg cggctggtgc tggagtcggt gctcgacatg ttgtccgggc gctatccctc
     9601 cgacgagttc gctgagttgc gcccccggat cgtctgggac cgcgtcgccg ggacgctcca
     9661 cgggcgccgc ggggcccagc acctcgccgt cacctcgggc ggcaccatcc ccgaccgcgg
     9721 cctgtatgcc gtgttcctcg cctctggcga cggtcccgga cgacgggtcg gtgagttgga
     9781 cgaagagatg gtctacgagt cgcgggtcgg cgacacgttc accctcggga cgtcgacgtg
     9841 gcggatcgag gacatcaccc acgaccgcgt tctagtgact ccggcgccgg gtcagccggg
     9901 caggctgccg ttctggaagg gcgacaccct cgggcggcca gtggagctcg gggccgcagt
     9961 cggtgcgttc gtgcgcgaag tcggcgggct ggccacggcc gcagcccgga agcgggtcgg
    10021 cgccgccggc ctggacgagt gggccaccga caacctgttg ggttacctca ccgagcagcg
    10081 cgaggcgatc ccccaactgc cgaccgaccg gatgatcgtc gtcgaacgat tccgcgacga
    10141 gctgggcgac tggcgcgttg tcatccactc ccctttcggc agcccggtcc atgccccgtg
    10201 ggcgttggcc gtgtcggcaa ggatgcggga gcggttcggc gtcgacgtgc aggcaatgca
    10261 tggcgacgac gggatcgtcc tgcggctgcc cgacctcgac ctgggtggtg aggttggtgt
    10321 caatgccgac gccggcagcg cgacgatcgg cgccgagctg acccaggagt tgctcgacct
    10381 catcctgctc gaccccgacg aggtcgccga cctcgtcacc gcccaggtcg gcggttcggc
    10441 cctgttcgcc tcgcggttcc gcgagtgcgc ggcgcgggcc ttgctactgc cccgccgtcg
    10501 acccgaccgc cgtcaggccc tctggcagca acgccaacgc gcctcgcagt tgctcgaggt
    10561 cgccagccgc taccccgcct tcccgatcgt cttggaggcc gtccgcgagt gcgtccagga
    10621 cgtgttcgac gtccccgggc tggtgggcct catgcgggcg atccgatccc gcgaggtcgg
    10681 ggcgttggcg gtgcagacca gggtgccgtc gccattcgcc cgctcgctgc tgttcggata
    10741 tgtcgctcaa tacctctacg aaggcgactc accgctggcc gagcgccggg ccgcagcgct
    10801 cgcgctcgac cccacgctcc tggccgagtt gctcggcacg ggcgacggcc tgtcgctgcg
    10861 tgacctgctc gacccggcgg ctgtggccac gaccgaggcc gagttgcagc ggttggtccc
    10921 cgagcgggcc gcccgcgacg ccgaggacct tgccgacctg gtccgggtct tggggcctct
    10981 gggggtcgac gatctggtcg ctcgcagcgt cacctcagat gcccgcgtcg tcggcgcgtg
    11041 gctcgtcgag ctcgagtcgg cgcggcgggt catccgcatc cgcatcggcg ggcaggagcg
    11101 atgggccgcc gtcgaggacg ccggtcggct gcgcgatgcg ctcggagtgt cgctgccagt
    11161 cggcgtgcct gaggtattcc tcgctccggt cgccgacccg ctgggcgacc tggtggcccg
    11221 attcgctaga agccacgggc cgttcggggt gcatgccgtc gcagcctggc tgggcctggg
    11281 cccggcggtc gttcgcgacg cactgcgccg gttggtcgcc gctgggcgcc tcgtcgaggg
    11341 cgagctgcgg ccccagggca gcgccgacgg cgggctcgac tactgcgacg ccggggtcct
    11401 gcgcacactg cgccgacggt ccctggccgc gttgcgggcc gaggtcgagc cggtcggccc
    11461 ggtcgactac gcccgcttcc tccccacctg gcagggcgtc ggcggaacgc tgcacggccc
    11521 cgaagggctg ctgcgggccg tcgaacagct cagcggcgca cttctcccag ccagtgccgt
    11581 ggagagcctg gtgctgccca gccgcgtcgc ggcataccag cccgcctggc tcgatgagtt
    11641 gctggcggcc ggcgaggtgc tctggcaggg gcacggcgcg ctccccggcg atgacgggtg
    11701 ggtgtcgctg catgtcgccg acctggcgcc gacgaccttg acccccgcga ccgcgctcgc
    11761 cgacctcggc gacattcacg ccgccatcct cgagtgcctc ggcaacggcg gggcctactt
    11821 cttccgcacc ctcatcgacg cggtgtccgc ggccgtgggt ccgagccccg acgaggccgt
    11881 ccgcaccgcg ctctgggagc tcgtctgggg cgggcagatc agcggcgaca ccctcgcccc
    11941 cttgcgtgcc ctccttggcg gggggcggac cgcccaccgg cgccgcgcga ccccagggcc
    12001 tcgtggccgg tatgccgccc gcggcacctc cctcggcctc ggcacctccc gcctcggttg
    12061 gggcgccgtc ggtggcgagg gcggggccag tgccggcggc gcagttggcg tcggcagcgg
    12121 tgtcggcggg gcggctcgcc tcggaggtgc gctcgggcgg gccgggcggt tcgcaccgac
    12181 cgctgcagat gtcgtgggcc gatgggcgct gcttccgggc cgcgagagcg acccgacggt
    12241 cacggcatac acgagggccg aggtgctcct ggaccgctat ggcgtcctga cccgcgggtc
    12301 ggttggtgcg gaagacgtcc cgggcggttt cgccggcgtc taccgcgtcc tggccgcggc
    12361 cgaggagtcc ggtcgggttc gtcgcggcta cttcgtcgaa gggctgggtg cggcgcagtt
    12421 cggctcgacc ggcgcagtcg accgccttcg cgcgggcgtg cgcccgttgg gggaatccgc
    12481 tcgcgccatc ctgcccgcgc tggtcctggc ggcctgcgac cccgccaacc cctacggcgc
    12541 ggcgttgccg tggccgggcc gggagccggt cgaaggcggc gggcgaggcc atcaaccggc
    12601 tcgcaaggcg ggcgccctcg tcgtgctggt cgatggtgaa ctggtcctct acgtcgagcg
    12661 gggcgggcgg tcgctgctca cctggagcga ggacccggcg gcgctgcagg cagcggccgg
    12721 cgccctggcc ttggcggtcc gcgagggtgc gctcggcaag ctcaccgtcg agcgcaccga
    12781 cggcgcttcg gtgctgggct cggaccacgc cctagccgct gcactggcgc aggctggttt
    12841 ccacgccacg ccccgtgggc tgcggctgcg gcgatgactc ggctcactcc cggagcccgg
    12901 ccgaaagctg agggccgcga tgcctgaggg tgacaccgtc tggcgtacgg cccagcgtct
    12961 gaacgaggcc ctggccggcg acccgctcgt ccacgccgac ctgcgctggc cctccctcgc
    13021 cttggccgac cttcgcggtg ccgtcaccct tgagatccga gcccggggca aacacctgct
    13081 ccatcgcctc gacaacggcc tcaccctgca ctcgcacctg cggatggagg gccagtggcg
    13141 gatcgaatcg acggccaccc tcagcgtgcg ccgattggcc gacgacgatc tccgggccgt
    13201 tctgggcacc acccggtgga cggcgctggg gctgcggctg gggatgctcg acctggtgac
    13261 gacctctgat gaggcgtcgc tcgtcggaca cctcggcccg gatgtgctgg gatccgactg
    13321 ggaccccgtg atcgccacct ccaacctcgc tgcctcggct tcgatgatcg gtgccgccct
    13381 gttggaccaa cgcaacctcg ccggcgtcgg cacgatatat gcggcggaaa gccttttcct
    13441 gcaaagactc tcgccgtggg cattggccac cgagctgcca acgacggcgg tcgcagcatt
    13501 ggtcgagcgg gctcaccaac tgctcgacat cgggcgccgc aatcccctcc agtcgaccac
    13561 cggccgcacc cgcaggggcg agacgacctt cgtccacggc cgatcgggtc ggccctgcct
    13621 gcgttgcggc acgatcatcc gtctggggat ggtcggcacc gctccgcagg agcgggcgat
    13681 gttctactgc ccgacgtgtc agggcgttcg cagcgttcag gacgttcgcg agtttcaggc
    13741 cgatcgcagc cttccgggtg atcgcagcgt tcagggcgtc cgcaccgacg gccgacgtcc
    13801 gccacgcccg tcaccttccc gcggccgcta gggtcgggcg agtgactgac gagcacttgg
    13861 gcggaccgac tgtcgtcgcg gcaggtgtct ccaagaccat cagcgacacg acccttctcg
    13921 cgcccaccga ccttcgcgcc gagtcaggca cctgtgtggt cgttcgcgga cgcaacggca
    13981 tcggcaagac caccttgctg cgcatcctcg ctggcactct tgagccgacg actgggacgg
    14041 cctcgatcga cggcgtccgg ctcgacgagc gtgatgcggc cacccgccga cgggtggccg
    14101 cgctcctcgg tgcgcccacg gcataccggg acctcaccct cgccgaccac ctgaccctca
    14161 tcgatgcgac gtggggacgg gacgtcgccg ggtgcgcgca gcgcgtggcc gacgggctcg
    14221 acgctctcgg catcggcgat ctcgcgcacc gcttcccgca cgaactttcc agcggtcaga
    14281 gccaactgtt tcggctggcc atggtgttct tccgcccggg cgacctgctc gtcctcgacg
    14341 agccggagca acgcctcgac acgacgaagc gagcgctcgt cggcgacctg ctcatccacc
    14401 gcaggcaggc cggggacacg ctgctggtcg cctgccatga tccggtgctc accgagcgga
    14461 tcgccgacgt cgtgctcgac ctgcccgagc cgtgaacgac cgggagcgcc tcgaggacgt
    14521 cgagcacgtc ctgcatggcg acaagggctc cggagcggcc aagaacatgg ggtatgccgt
    14581 ctacgtcacg gtgctgctcg ccagcacgta tggcttcacg gtggcgcgcg ccgtcttcgc
    14641 gaccagcggc ccggcgtgga tcgacgacca cgtgctgacc ctccctaccg ccgtgatcgg
    14701 tgtcggtctc ttcgtcgcgt cgctcgccct cgcccgatgg gctggccgga cccgtggccc
    14761 cgtcgtgccg ccactgccgt ggaccgacct agtcgcctcg agtgacgtcg accgggcccg
    14821 ctcgctgcgt cgatggtgga cctatgcctt tgtcggcggc ctggttggcg gcgcgatcct
    14881 tggcgccgtc atcggtggag gggtgtgggc ctcgggcgcg ggtagcgcgg ggtggttcgt
    14941 gggtgcggtg ccgattgggg cggcggccgg atggctgctc gcgaggatgt ggctcgaggg
    15001 ccaggtggcg ccggatacct cgggaacggc gacgctcgct cgggttgtgc ggccagccgc
    15061 ggggtccttg cggcagttgc gactggacga cctgcggtcc cacggtgttc gctcgaccca
    15121 gatgggtggc ggagtgttgg cgggannnnn nnnnngagac ctgcgggcgg tccgccttga
    15181 ggtggcctcg ccggtcaccc gcggccgccg gttgcgcctg caggctgcgg gaccgcgcgc
    15241 ggtagttgtg cggcgcgaca ttctcggtct ccggcgggcg ccaggagcct tctggactgg
    15301 ggccgtcctc gtggtcatcg ggtgggctgg ggtcgtgtgg gccctcaccg acccgcgggt
    15361 gccagtggcg ctcacgtgcc tcgccatggc ggcgtgctac ctggggatgg gggcgtgggc
    15421 cgaagggttg cgactgctcg gtgacaacgc cggcacgcca ccgatgctgg gtctgcgctt
    15481 tcggaccgag gcgattgcgc acctcgtggt gccggtcgcg cttttcctca tgacgaccgt
    15541 cggggtgagc gccatcggcc tgatggccat gaacaacgtg ggagtggacg gcgcgggact
    15601 gagcagcgcg ggaccaaatg gggcggtgcc catcctcgct gtcctcgggt ggtgtctgct
    15661 catcgggact ttgctcgccg gagctcagct gatggccgcc ttccggggag cgccgccggt
    15721 catggcgttc ctccccgagt acggcccacc gatgatgatc tattggtacg cccggccgct
    15781 cgtcctggcc tcggtgtccg ggggcctgat cgtctcccac gccactgcag gggcagccgc
    15841 cagcaccgct gccggctccg gcatggtcac cggcatgctc attgccttgg tgacggcaat
    15901 cggggccatg gcctggggcc tacgcagcgt gaagaccctc gaagaggccc atcgcatctg
    15961 atcgagttcg gcgggccttc cgatctgggc gtcggccgcc tgagcggtct cgtcgtctca
    16021 agcctcctcg gcgagactct cgaacagccc cttcgcgccg caaactgccc ctcgtgcggc
    16081 ctccccggct aagcggccgg gcccgcgtcg aaccagggtt tgcttcggg
//
LOCUS       DAOMZY010000025        16112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_3258_length_16112_cov_5.604845, whole genome shotgun sequence.
ACCESSION   DAOMZY010000025 DAOMZY010000000
VERSION     DAOMZY010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 16112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16112
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3258_length_16112_cov_5.604845"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="PLK64_02700"
     CDS             <1..847
                     /locus_tag="PLK64_02700"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPV78680.1"
                     /translation="PAVALPQRPATLPMPRVLAIVASTDLARGLAENPETRPDGFVVE
                     GAAAGGHNAPPRGPRRVDEIGQPVYDERDVVDVADILSLGLPVWLAGGYGTPDKVAEA
                     LALGAAGVQVGTLFAYSDESGFDADVKAQMREKAVAGELQVRADWRVSPTGFPFRVAD
                     IPGTLTDVTVRAARKPVCDLGVLRSAYLKADGEVDYRCPAEPLAQYVRKGGREMNTEG
                     RLCLCNALFASAGFPQRRPGGASEAPLVTSGTDLEPVVHLIGASDGPGYGAADAVAHL
                     LSRIS"
     gene            873..1745
                     /locus_tag="PLK64_02705"
     CDS             873..1745
                     /locus_tag="PLK64_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPV78681.1"
                     /translation="MRVSTYAAPPGPWTPGRPAVVGHRGARGLIPENTVASMRAAIDV
                     GVPAIELDVLLTADDQVVVWHDPVLHPAKCRPTGVDYLGARVDELTLTQLRTVDVGSL
                     TLAGFPRQRAVPGERIPTLAEVFAATTDAPIWWVIEVKIDVTDPRDRAARHTLLDGVL
                     TAVTDAGVRDRVFIHSFDWAVLEQARELDPQLPRSALAVVGETFAPGSEWLGSVRWED
                     HPNDLVGAVAELGAVLVSPDFHSCTAELVEQAHARGMAVLPWTVNDPVIFGDLADLGA
                     DGVVTDLPDEMLAL"
     gene            1771..2229
                     /gene="mscL"
                     /locus_tag="PLK64_02710"
     CDS             1771..2229
                     /gene="mscL"
                     /locus_tag="PLK64_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HPV78682.1"
                     /translation="MKNAWKEFKAFAMGGNMLDLALGFIIGAAFATLVESLASNILMQ
                     LVAAIFGTPDFTELSLTLNNAEITYGAFLTDFVGFILMALVLFAIVKLLKKAGMGNFR
                     AQGQRECPMCKEFVAVDALKCKWCTADIDPDQAEEEDLEIIEARRKPEQP"
     gene            2226..2555
                     /locus_tag="PLK64_02715"
     CDS             2226..2555
                     /locus_tag="PLK64_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HPV78683.1"
                     /translation="MSADYAFRAPLWRWQGESPWHFVTLPEEQSDEIDERTRDVQRGF
                     GSIRVEVTVGTTVWATSLFPSTNHGAYLLPVKKAVRVAEGLTADEPVDVRLRLLDLPG
                     GAPGSRG"
     gene            2901..6203
                     /gene="ileS"
                     /locus_tag="PLK64_02720"
     CDS             2901..6203
                     /gene="ileS"
                     /locus_tag="PLK64_02720"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPV78684.1"
                     /translation="MVYPKASTDPEATGVPPTPRFPTIEERVLAYWAKDGTFLASVEA
                     RDGGTDGSNEFVFYDGPPFANGLPHYGHLLTGYVKDLVPRYRTMRGRRVERRFGWDTH
                     GLPAELEAQRQLGLKTTQEITDLGIATFNDACRNSVLRYTKEWEEYVTRQARWVDFEH
                     DYKTLDTDYMESVIWAFKQLHEKGLAYEGFRILPYCWNDQTPLSNHELRMDDDVYQMR
                     QDPAITVGYRLETGELALIWTTTPWTLPSNMGVAVHPDVDYVVVQGDKDGGSDRYLLA
                     QARLAAYTRELGDDAAERIVARYKGSELVGRRYTPPFTYYLGYAGAHQISAAGFVTTE
                     DGTGLVHMSPGFGEDDKLALDALGVETVVPVGPDGCFVFPVTDYAGLHVFDANHPVID
                     HLKARTRGHGEMGSTTEGTVLVRHETYDHSYPHCWRCREPLIYMAVSSWFVATTAIRD
                     RMLELNQEITWVPEHTKDGQFGKWLENTRDWSISRNRFWGSPIPVWKSDDPTYPRVDV
                     YGSFAELERDFGVPVTDLHRPFIDSLTRPNPDDPRPEGERSTMRRVTDVLDCWFESGS
                     MSFAQVHYPFENADWFEHHFPGDFIVEYIGQTRGWFYTMHVLATALFDRPAFKTCISH
                     GIVLGSDGQKMSKSLRNYPDVFEVFDRDGADAMRWFLMSSPILRGGNLVVTEGGIRDG
                     VRQVMIPLWNAWHFFALYANAAGGGKGYAATASTASTDLLDRYLLAKLREFVEAMTAQ
                     LDSYDIAAACDTMRSFLDVLTNWYIRRSRDRFWDSDNSLDASTTAAFDTLYTALEVLT
                     RAVAPLLPLTTEEIWRGLTGGRSVHLEDWPDAAALPADHALVAGMDRAREVCSAVSSL
                     RKGASLRARLPLAELVVVTTDPAALEPFSAIIADEVNVRSVVLRDVELAHESDFGVMR
                     RLSVNARVAGPRLGKEVQTAIRAAKAGDWSTAPDGTVTAGGIALVDGEYAVETVVAGQ
                     DARTAGSTDQGSGPSHAVAVLPAGGFVVLDTEVTPELAVEGVANDLVRAVQQTRRESG
                     LHVSDRISLSLAGAELMQRAAHEHRDRLMAQTLAEHLSVATHVEDLALGDGVVETSVG
                     DALPVRIKVAKR"
     gene            6203..6700
                     /locus_tag="PLK64_02725"
     CDS             6203..6700
                     /locus_tag="PLK64_02725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV78685.1"
                     /translation="MGVVIRPATAYDVDAVVDVGRRTWPQTYTPLAGEDYVRMGLDKW
                     WTVAATRPLVLAGKATVAELDGEVVGVAVVGPLTGDLVLFRLYVVPEHQGMGIGRLLL
                     EDVMTTARETGHRILRLSYLDGNAGAERFYQGFGFVESHREPTGDGIPDSIWVVRDLW
                     EQEQS"
     gene            6697..8061
                     /locus_tag="PLK64_02730"
     CDS             6697..8061
                     /locus_tag="PLK64_02730"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HPV78686.1"
                     /translation="MTPPPSDPAVAARLAQVEAEIMGRAPEHDLVPTLDRVAAVMELL
                     GDPQRSFRVIHLTGTNGKTSTTRMIEALCREHGLRTGRFTSPHLHSMRERIAVDGEPV
                     SAEVLVETWDEVAPIIEIVDARSAAEGGPRMTFFEVLVVLAYAVFADAPVDVAVVEVG
                     MGGRWDATNVADGDVAVVTPIDIDHTHFLGSTVEEIAFEKAGIVKPGAVLVTAAQDEA
                     VAAILTDRCDEVGARRYAADEEFAVTDREVALGGQLLAIRGVAGNYDEIFLPLHGEHQ
                     GANAALALVAVELFLGGGERRLDPEVVRAGFAAVTSPGRLEVVRRSPTILVDAAHNPH
                     GATALALALGEAFSFARLVGVVAIFADKDAGAMLEALEPALDHVVVTRNSSPRSMNVA
                     SLGEIASGIFGAERVSVVPALPDALEEAVTVAESEGMGGGVVVTGSVVTAADVRLLLG
                     VKET"
     gene            8061..8459
                     /locus_tag="PLK64_02735"
     CDS             8061..8459
                     /locus_tag="PLK64_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4233 domain-containing protein"
                     /protein_id="HPV78687.1"
                     /translation="MAGIVFFGTLGKFTWRMCAAVLAAQSIAVFFGALVARGIAVADS
                     SGAATAYLLVGSGLAILCVIAAGAMRSPFGVTLGWLIQVATFAATVIVPLMGLVGVIF
                     GALWVLCLIQGHRIDGLQRAHAQESAAPEE"
     gene            8470..8892
                     /gene="ndk"
                     /locus_tag="PLK64_02740"
     CDS             8470..8892
                     /gene="ndk"
                     /locus_tag="PLK64_02740"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPV78688.1"
                     /translation="MTTERSLVLVKPDGFARGLTGEVIRRIERKGYRLVALRILTPTR
                     EMLGRHYAEHAEKPFFGELVDFMSSGPVTAIVIEGNQCVPGFRSLAGATDPTKAAPGT
                     IRGDLGRDWGKGSTENVVHGSDSPESAAREMAIWFPDL"
     gene            complement(8899..9351)
                     /locus_tag="PLK64_02745"
     CDS             complement(8899..9351)
                     /locus_tag="PLK64_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPV78689.1"
                     /translation="MIPLDELVGRAEALARSAHAGQVDKAGAPYAQHPERVAAAVTGD
                     PQAQAAAWLHDVVEDTSTSLADLRAAGFPEPVIAAVDALTRRPDEPGDNYYARVAADP
                     LALRVKRADIADNSSHERLGRLDEATQIRLRHKYAHALATLAAYELPR"
     gene            9494..10615
                     /locus_tag="PLK64_02750"
     CDS             9494..10615
                     /locus_tag="PLK64_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HPV78690.1"
                     /translation="MAALAFRDPRLEAAVERAWARSRLSLRTRVDRLVDKSWHVVQCA
                     VAAGVAWWFASGVLHHAMPFFAPIVAVVCLGMTYGQRLRRVAEVTVGVAVGIAVADIF
                     VGVVGSGPWQITVVVLVSMSLALLLDAGLLLVMQSAVQSIVVTVLVPTGSQALGRWVD
                     AVIGGVVALVFAMVVPRAPLRRPRRQAARVGRVMARLLREAAQSAREGDVERSAQVLA
                     DARQTDPLIRELQAAADEGMSVIASTPFTRTDAGSVRSIASVVDPLDRAMRSTRVLVR
                     RMSIAADSDIEVPDDYVDVIVALADAVDILDRVWAENRSAEFARPGFVALAERTATLD
                     PGEYHTTVILAQLRSLVVDLLELSGMDHEDAVAAVPPLG"
     gene            complement(10705..>10809)
                     /locus_tag="PLK64_02755"
     CDS             complement(10705..>10809)
                     /locus_tag="PLK64_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HPV78691.1"
                     /translation="HAGLRQTIDWYAANEAWWRTAKESTEQVYARLGR"
     assembly_gap    10810..10909
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10910..11506)
                     /locus_tag="PLK64_02760"
     CDS             complement(<10910..11506)
                     /locus_tag="PLK64_02760"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPV78692.1"
                     /translation="MKVLVTGGAGFIGANFVLRVRETRPDWEVTVLDALTYAGNRASL
                     APVEGQIEFVQGSIADADLVDRLVADTDLVVHFAAESHNDNSLDNPWPFMEANIIGTY
                     RLLEAVRKHGKRIHHISTDEVYGDLELDDPNRFSESTPVNPSSPYSASKASADLLVRA
                     WIRSFKIAATLSNCSNNYGPRQHVEKFIPRQITGILDGV"
     gene            complement(11503..12288)
                     /locus_tag="PLK64_02765"
     CDS             complement(11503..12288)
                     /locus_tag="PLK64_02765"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPV78693.1"
                     /translation="MTSSRFSTIAHDLRERVALGDVGVHGALESETELCARYGVSRPT
                     VRRALETLRDEGLVQSRHGAGWFVTGTAFHQRLALGTFRHAGSAVAESGASVCREVAD
                     FSFRPAPSGVATILQIAVGAPVLHVRSARRVDGESLDVAHEWVSEQFAGRLSRADAAD
                     PGLWASLTRDGHSIATVHQTITAGLASGADADLLHTAVGVPLLLVRRVAMGADGIPLA
                     LSDHRYLAHRFALEVEFNSGPSASSPEPPGLRAVGDTPQEIPS"
     gene            12350..13123
                     /locus_tag="PLK64_02770"
     CDS             12350..13123
                     /locus_tag="PLK64_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78694.1"
                     /translation="MTRLRGMVLIGGLVPLLSGCLVVSALNASNGPATGAPTSIATFD
                     PFATGAVPKATGATPSPSPSPTPSTSSSPSPKPSASPPPPALPAGAKWATVDSEQVRF
                     AVASAWVEIVPATMTTTASIPADVKAIAKALGISARELIDRFDDIEIAYVAAPVNRYA
                     TNLSATVADVGSLPDDDWVKTTYSEGKVKVLGSERLATRVGDALRINGTVTVQGASIA
                     LTTFIVDVGRHCIVFEVAARKRADVDSAIARLASTLHEI"
     gene            13131..13424
                     /locus_tag="PLK64_02775"
     CDS             13131..13424
                     /locus_tag="PLK64_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78695.1"
                     /translation="MALPWWTLFVLAAAACSWISVTVLARNNPGERLSWFRSPTVAPL
                     SQLWFLAASVACAWLGGSYAADTALGGWAYPIAALAVLVPWALVRAGHNRHVG"
     gene            13525..14766
                     /locus_tag="PLK64_02780"
     CDS             13525..14766
                     /locus_tag="PLK64_02780"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV78696.1"
                     /translation="MAVEPALRRPEVRRVITSHAFAAVGMGLPWPVLLLAVEQSTSSQ
                     LLLGLAGAARLAPYVALSWLSGRLADRRERALIVRISLWARAVALLVAAAALAASNPL
                     VAVVAATGAIVAGTPAYPALAAGMPRLAGAATERATSALVTVEVASFVVGPAVGGLFV
                     GRMPPAWVPLIGAIFVGVAIVAFGRTAMPAPEASAIDVAEGGVWSLLRRDPCARQALA
                     VVAVVNALLSVLGLLLLQLAHEVWRSGDEGFGLATALLGFGALGAPVVAHSRTMRSGR
                     AALLTLAGAAAVFALLPGGPASFGALAVIGAVAVCCENVATSVLQATVPDELRASVLG
                     LGDTVMVGAALLAAVATPWLGVHLGPASLLLLVAAGTAMLALAWSAGRQGPDSVATLP
                     GGSGEELPVHHDEVGVRVPGV"
     gene            14977..15489
                     /locus_tag="PLK64_02785"
     CDS             14977..15489
                     /locus_tag="PLK64_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HPV78697.1"
                     /translation="MILPFGPHTPSIAAGAWVAPTAMVIGDVVIEEGASIWYGAVIRA
                     DRARIRIGARSNVQDNAVFHADPGAPVDLGCDVTVGHGAIVHGATVGDGSLIGMGATL
                     LNHSVVGGGSLVAAAALVREGQVIPPGSMVAGVPAKVLGPVPAGFDVHVSGSVATYET
                     LRDEHRGATP"
     gene            15499..>16112
                     /locus_tag="PLK64_02790"
     CDS             15499..>16112
                     /locus_tag="PLK64_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPV78698.1"
                     /translation="MADFDVVVLGAGPGGYVAAIRAAQLGLKAAVVELKYWGGVCLNV
                     GCIPSKALLKNAELAHVLTHEKAKYGIEGDATMSYGPTHARSRQVSAGIIKGVHFLMK
                     KNKITEIDGWGTLTSPTSMDVKAADGSARSITFDNLIIATGSVTRMIPGVQVSANVVT
                     YEEQILDPNLPGSIIIAGSGAIGVEFAYVMRNFGVDVTIVEFLDR"
BASE COUNT         2288 a         5525 c         5602 g         2597 t
ORIGIN      
        1 ccccgccgtt gcgctgcctc agcgtccagc caccttgccg atgccccgcg tgctcgccat
       61 cgtcgcctcc accgacctgg cccgcggcct ggccgagaac cccgagaccc gccccgacgg
      121 tttcgtcgtc gagggcgcgg ccgccggtgg acacaacgcc ccgccccgcg ggccgcggcg
      181 cgtcgatgag atcgggcagc cggtctacga cgagcgcgac gtcgtcgatg tcgccgacat
      241 cctctccctc ggactgccgg tgtggctggc cggtggctac ggcacccccg acaaggtcgc
      301 cgaagccctc gcgctcggcg ccgccggggt ccaggtcggc accctgttcg cctacagcga
      361 cgagtcgggc ttcgacgccg acgtcaaggc ccagatgcgc gagaaggccg tcgctggcga
      421 gttgcaggtt cgggccgact ggcgggtgtc gccgacgggc ttcccgttcc gcgtcgccga
      481 catccccggc acgctcaccg acgtcaccgt ccgggccgcc cgcaagccgg tgtgtgacct
      541 cggcgtcctg cgctcggcct acttgaaggc cgacggcgag gtcgactacc gctgcccggc
      601 cgagccgctc gcgcagtacg tccgcaaggg cgggcgtgag atgaacaccg agggccggct
      661 gtgcctatgc aacgcgctgt tcgccagcgc cgggttcccg cagcgccgcc ccggcggcgc
      721 gtccgaagcc ccgttggtca cctccggcac cgacctcgag ccggtcgtcc acctcatcgg
      781 tgcgagcgac ggtccgggct acggtgcggc cgacgccgtc gcgcacctgc tgtcgcgcat
      841 cagttgagcg gttctccggt tagcgttgcg gcatgcgcgt cagcacgtat gccgctccgc
      901 ctggaccgtg gacaccaggc cgcccggccg tcgtcgggca ccgcggtgcc cgagggctga
      961 tccctgagaa caccgtggcc agcatgcgtg cggccatcga cgtcggcgtt ccggccatcg
     1021 agttggacgt cctgctcacc gccgacgacc aggtcgtcgt gtggcacgac ccggtgctgc
     1081 atcccgccaa gtgccggccg accggcgtcg actacctcgg ggctcgggtc gacgaactca
     1141 ccctgaccca actgcgcacc gtcgacgtcg gctcgttgac gcttgcgggc tttccccggc
     1201 aacgggcggt gccaggggag cggatcccca cgctcgccga ggtgttcgcc gccaccaccg
     1261 atgcacccat ctggtgggtc atcgaggtca agatcgacgt gaccgacccg cgcgaccggg
     1321 ctgcgcggca taccctcctc gacggcgtcc tcaccgcggt gaccgacgcg ggggtgcgtg
     1381 accgggtctt catccactcc ttcgactggg ccgtcctgga acaggcccgc gagctcgacc
     1441 cgcagctgcc gcggtcggcg ctggctgttg tcggcgagac gttcgcgccc ggcagcgagt
     1501 ggctcggatc ggtccgttgg gaggaccacc cgaatgacct cgtgggggcg gtagccgaac
     1561 tcggggcagt cctcgtgtcg cccgactttc attcctgcac agccgagttg gtcgagcaag
     1621 cccacgcccg agggatggcc gtgctgccct ggaccgtcaa cgacccggtc atcttcggag
     1681 acctcgccga cctcggcgcc gacggggtgg tgaccgacct gccggacgag atgctggcgc
     1741 tctaaccggc gccgccggac aatgtcggcc atgaagaacg cgtggaaaga gttcaaggcc
     1801 ttcgccatgg gcggcaacat gctcgacctg gcgctcggct tcatcatcgg ggcggccttc
     1861 gcgaccctcg tcgagagcct ggccagcaac atcctcatgc agttggtggc cgcgatcttc
     1921 ggcacgcccg acttcaccga actgagcctg acgctcaaca acgccgagat cacctacggc
     1981 gcgttcctca ccgacttcgt cggcttcatc ctcatggcgc tcgtgctctt tgcgatcgtc
     2041 aaactgctca agaaggccgg catgggcaac ttccgggccc agggccagcg cgagtgcccg
     2101 atgtgcaagg agttcgtcgc cgtcgacgcg ctcaagtgca agtggtgcac cgctgacatc
     2161 gacccggacc aggccgagga ggaggacctc gagatcatcg aggcccgacg caagcccgag
     2221 cagccttgag cgcggactac gcgttccgcg cgccgttgtg gcggtggcaa ggcgagagcc
     2281 cgtggcactt cgtcacgctg cccgaggagc agtccgacga gatcgacgag cgcacccgtg
     2341 acgtccaacg gggctttggc tcgatccggg tcgaggtgac cgtcgggacg acggtctggg
     2401 cgacctcgtt gttcccgtcg accaaccatg gggcctacct gctgcccgtc aagaaggccg
     2461 tccgggtcgc cgagggcctg acggccgacg agcccgtcga cgtgcggctg cggttgctcg
     2521 atctgccggg cggagcaccg ggctcacgcg gttaaccgtt cggcgacgtc cccgcactca
     2581 cgtagaatca ttggcagcgg cgacgacccg gccatcaccg gcgagcctcc ggaagaaccg
     2641 tccacctcca cgcgggggtg ggctcagtag aaccggacgg gtggcccgtc acagccggaa
     2701 cgacaagagc ggtcgtatgc cgcgtgctcc ccaccgggcg aaacccgggc ggtgcgcggg
     2761 cggcatacga caagcgaggt ggtaccgcgg caactccggt ggtgatcgac gtgatcaccg
     2821 tgatccggga gcgtcgtcct cgtggcacgt aggagccggg cgaccggcat accggaccga
     2881 gagacgaggc aggcagacca gtggtctacc ccaaggcgag caccgacccc gaggcaacgg
     2941 gcgtgccccc gaccccccga ttcccgacga tcgaggagcg ggtcctggcc tattgggcca
     3001 aggacgggac gttcctcgcg agcgtcgagg cgcgcgacgg cggcaccgac gggtccaacg
     3061 agttcgtctt ctacgacggg cctcccttcg ccaacggcct gccgcactac gggcacctgc
     3121 tgaccggcta cgtcaaggac ctcgtcccgc gctaccgcac catgcggggc cgtcgggtcg
     3181 agcgccgctt cgggtgggac acccacgggc tgcccgccga gcttgaggcg cagcgccagt
     3241 tggggctcaa gaccacgcag gagatcaccg acctggggat cgccaccttc aacgacgcct
     3301 gccgcaactc ggtgctgcgc tacacgaagg agtgggagga gtacgtcacc cgccaggccc
     3361 ggtgggtcga cttcgaacac gactacaaga ctctcgacac cgactacatg gagtcggtga
     3421 tctgggcctt caagcagttg cacgagaagg gcctggccta cgaaggcttc cggatcctgc
     3481 cctactgctg gaacgaccag accccgctgt ccaaccacga gttgcggatg gacgacgacg
     3541 tctaccagat gcgtcaggac ccggcgatca ccgtcggcta ccggctcgag accggcgagc
     3601 tggccctcat ctggacgacg accccgtgga ccctgccgtc gaacatgggc gtcgccgtgc
     3661 accccgacgt cgactatgtc gtcgtgcagg gtgacaagga cggcggcagc gaccgttacc
     3721 tcctcgcgca ggcgcggctc gcggcataca cccgcgagct cggggacgac gcagccgagc
     3781 ggatcgtcgc gcgctacaag ggatcagagc tggtcgggcg ccgctacacg ccgccgttca
     3841 cctactacct ggggtatgcc ggcgcgcacc agatctcggc ggcgggcttc gtcacgaccg
     3901 aggacggcac cgggctggtc cacatgagcc ccggcttcgg cgaggacgac aaactcgccc
     3961 tcgacgccct cggggtggag accgtcgtgc cggtgggccc ggacgggtgc ttcgtcttcc
     4021 cggtgaccga ctacgccggg ttgcacgtct tcgatgccaa ccacccggtc atcgaccacc
     4081 tcaaggcccg cacccgcggg cacggtgaga tgggctccac gaccgaaggc accgtcctcg
     4141 ttcgacacga gacctacgac cactcctacc cgcactgctg gcgctgccgc gaaccgctga
     4201 tctacatggc ggtctcgtca tggttcgtcg cgacgacggc gatccgcgac cggatgctcg
     4261 agctcaacca ggagatcacc tgggtgcctg agcacaccaa ggacggccag ttcggcaagt
     4321 ggctggagaa cacccgcgac tggtcgatct cccggaaccg cttctggggc agcccgatcc
     4381 cggtgtggaa gtccgacgac cccacctacc cgcgagtgga tgtctatggc tcgttcgctg
     4441 agttggagcg cgacttcggc gtgcctgtca ccgacttgca ccggccgttc atcgactcgc
     4501 tcacccggcc caaccccgac gacccgcgcc ccgaggggga gcgctcgacg atgcgccggg
     4561 tcaccgatgt cctggactgc tggttcgagt cggggtcgat gagcttcgcc caggtgcact
     4621 acccgttcga gaacgcggac tggttcgagc accatttccc cggcgacttc atcgtcgaat
     4681 acatcggcca gacgcggggc tggttctaca ccatgcatgt cctggcgacg gcgttgttcg
     4741 accgcccggc gttcaagacc tgcatctcac acggcatcgt cctgggctcg gacggacaga
     4801 agatgagcaa gagcctgcgc aactaccccg acgtcttcga agtcttcgac cgcgacggcg
     4861 ccgatgcgat gcgctggttc ctcatgtcca gcccgatcct tcgcggcggc aacctcgtcg
     4921 tcaccgaggg cggcatccgc gacggcgtgc gccaggtgat gatcccgctg tggaacgcct
     4981 ggcacttctt cgcgctctac gccaacgccg ccggcggggg caaggggtat gccgccacgg
     5041 cctccaccgc gtcgacggac ctgctcgacc gctacctgtt ggccaagctg cgcgagttcg
     5101 tcgaggcgat gacggcgcag ttggacagct acgacatcgc ggctgcctgc gacaccatgc
     5161 gcagcttcct cgacgtcctg acgaactggt acatccgccg ctcgcgggac cgcttctggg
     5221 acagcgacaa ctcgctcgat gcctcgacga cggccgcgtt cgacacgctc tacaccgcgt
     5281 tggaggtgct cacccgcgcg gtcgccccgt tgctgccgct gacgaccgag gagatctggc
     5341 gcggcctcac cgggggtcgc tcggtccacc tcgaggactg gccggacgcc gccgcgctgc
     5401 ccgccgacca cgccctcgtc gctggcatgg accgcgcccg ggaggtgtgc tccgcggtgt
     5461 cgtcgttgcg caagggcgcg agcctgcggg cccgcctgcc gctggccgag ttggtcgtcg
     5521 tcacgaccga tccggctgcg ctggagccct tctcggcgat catcgccgac gaggtcaacg
     5581 tgcggtctgt ggtcctgcgc gacgtggagt tggcgcacga gagcgacttc ggcgtgatgc
     5641 gccgcctgtc ggtcaacgcc cgggtcgccg gtccgcgcct cggcaaggag gtccagaccg
     5701 cgatccgcgc cgccaaggcg ggggactggt cgacggcccc ggatggcacc gtcaccgccg
     5761 ggggcatcgc cctcgtggac ggcgagtatg ccgtcgagac ggtcgtcgcc ggtcaggacg
     5821 ccaggaccgc cgggtcaact gaccagggct ccggtccctc gcacgcggtc gccgtgctcc
     5881 cggccggcgg gttcgtcgtc ctcgacacgg aggtcacccc cgagttggcc gtcgaaggtg
     5941 tcgccaacga cctggtccgg gccgtccagc agacccgccg tgagtcgggc ctgcacgtca
     6001 gcgaccggat ctccctgtcg ctggcgggcg ccgagctcat gcaacgggcc gctcacgagc
     6061 accgggaccg gctcatggcc cagaccctcg ccgagcacct gagcgtcgcc acccatgtcg
     6121 aggacctggc tctcggtgac ggcgtcgtcg agacgtcggt cggcgacgcc ctgcccgtcc
     6181 ggatcaaggt ggccaagcgc tgatgggtgt cgtgatccga ccggcaacgg catacgacgt
     6241 cgacgcagtc gtcgacgtcg gtcggcgcac ctggccgcag acctacaccc cgctcgccgg
     6301 tgaggactac gtgcggatgg ggctggacaa gtggtggacc gtcgccgcca cccggccatt
     6361 ggtcctcgcc ggcaaggcga ctgtcgccga gttggacggt gaggtcgtcg gcgtcgccgt
     6421 cgtggggccg ctcacgggcg acctcgtcct gttccggctc tacgtcgtcc ccgaacacca
     6481 agggatgggg atcggccggt tgctcctgga agacgtcatg acgaccgccc gcgagacggg
     6541 gcaccggatc ctgcggttgt cctacctgga cggaaacgcc ggcgccgagc gcttctacca
     6601 aggcttcggc ttcgtcgagt cccatcggga accgaccggg gacggcatac ccgactcaat
     6661 ctgggtcgtg cgcgacctgt gggagcagga gcagtcgtga cgccgccccc gagcgacccc
     6721 gcggtggctg cccgcctggc ccaggtggag gctgagatca tgggccgcgc ccccgaacac
     6781 gacctcgtgc ccaccctcga tcgggtcgcg gcggtcatgg agttgctggg cgacccgcag
     6841 cgcagcttcc gggtcatcca cctgaccggc acgaacggca agacctcgac cacccgcatg
     6901 atcgaggctc tgtgccgcga acacggtttg cgcacaggcc gattcacctc cccacacctg
     6961 cactccatgc gggagcggat cgccgtcgac ggtgagcccg tgtcggctga ggtgctggtg
     7021 gagacctggg acgaggtggc cccgatcatc gagatcgtcg acgcgcgctc ggctgccgag
     7081 ggtggccccc ggatgacgtt cttcgaggtc ctcgtcgtgc tcgcctacgc cgtcttcgcc
     7141 gacgcgcccg tcgatgtggc ggtcgtcgag gtggggatgg gcggccgttg ggatgcgacc
     7201 aatgtcgctg acggcgacgt tgccgtcgtg acgccgatcg atatcgatca cacccacttc
     7261 ctcggctcga ccgtcgagga gatcgccttc gagaaggcgg ggatcgtcaa gccgggtgcc
     7321 gtcctagtta cggctgccca ggacgaggcg gtcgccgcga tcctcaccga ccgctgtgac
     7381 gaggtcggag cccgccggta tgccgcggac gaagagttcg cggtcaccga ccgtgaggtg
     7441 gccctcggag gccagttgct ggcgatccgc ggggtggccg gcaactacga cgaaatcttc
     7501 ctgcccctgc atggcgagca ccagggcgcc aatgccgcct tggccctcgt cgccgtcgag
     7561 ctcttcctcg gcggaggtga gcgacgcctg gaccccgagg tggtccgcgc cgggttcgcc
     7621 gcggtgacct ccccgggccg gctcgaggtc gtgcgtcggt cgccgacgat cctcgttgac
     7681 gcagcccaca acccgcatgg ggccacggcc ttggcgctgg ccctgggcga ggcgttctcc
     7741 ttcgcccggt tggtcggcgt cgtggcgatc ttcgccgaca aggacgcggg cgccatgttg
     7801 gaggcgctcg agccggccct tgaccatgtg gtcgtcactc gcaacagctc gcctcgctcg
     7861 atgaacgtcg cttctctggg cgagatcgcc tcgggcatct tcggcgccga gcgagtgtcg
     7921 gtcgtccccg cccttcccga cgcgctcgaa gaagcggtga ccgtcgccga gtccgagggc
     7981 atgggtgggg gagtcgtcgt gaccggatcg gtcgtcaccg cggctgatgt gcgactcttg
     8041 ctcggggtga aggagacctg atggccggca tcgtgttctt cggcaccctg ggcaaattca
     8101 cctggcggat gtgcgcggct gtcctcgcgg cgcagtcgat cgcggtcttc ttcggtgcct
     8161 tggtggcccg cgggattgct gtcgccgaca gcagcggggc ggcgacggca tacctgctgg
     8221 tcggaagcgg cctcgcaatc ctctgcgtca tcgcggccgg ggcgatgcgc tcgccgttcg
     8281 gggtgaccct cggctggctg attcaggtgg cgaccttcgc ggcgaccgtg atcgtccccc
     8341 tcatgggcct ggtcggcgtc atcttcgggg cgctgtgggt gctctgcctc atccagggac
     8401 accgcatcga cgggttacag cgcgcacacg cgcaggagtc tgccgccccg gaggaatagg
     8461 gtgggccgcg tgactactga gcgctccctg gtcctggtca agcccgacgg tttcgcccgc
     8521 ggccttaccg gtgaggtgat ccgtcgaatc gaacgcaagg gctaccgcct ggtcgccctg
     8581 cgcatcctca cgcccacccg cgagatgctc ggccggcact acgccgagca cgccgagaag
     8641 ccgttcttcg gtgagttggt cgacttcatg tcctccggcc ccgtgacggc gatcgtcatc
     8701 gagggcaacc agtgcgtccc gggcttccgg tcgttggctg gcgcgaccga ccccaccaag
     8761 gcggcgccgg gcacgatccg tggagacctc gggcgcgact ggggcaaggg ctcgaccgag
     8821 aacgtcgtcc acggctccga ctccccggag tcggccgccc gcgagatggc gatctggttc
     8881 cccgacctct gagtcgcgtc accgcggcag ttcgtatgcc gcgagcgtcg ccagcgcgtg
     8941 tgcgtacttg tgccgcaggc ggatctgggt ggcctcgtcc aggcgaccga gtcgctcgtg
     9001 gctgctgttg tccgcgatgt cggctcgctt gacccgcagc gccaacgggt cggccgcgac
     9061 ccgggcgtag tagttgtcgc cgggctcgtc ggggcgccgg gtcagggcat cgaccgcggc
     9121 gatcaccggc tcggggaagc cggcggcacg caggtcggcc aacgaggtgg acgtgtcctc
     9181 gacgacgtca tgcaaccagg ccgccgcctg agcctgcggg tcacctgtca cggccgccgc
     9241 cacccgttcc gggtgctggg catagggtgc gccggccttg tcgacctggc cggcgtgggc
     9301 cgaccgagcg agcgcttcgg ctcgccccac gagctcatcc agagggatca tccgttggcc
     9361 tttcgacgtc gcgtcgggtc gggtcggggc gagccgcgcg gcatacctgc ggcccatccg
     9421 agccgcatcc ctcgccgatc acggctcgtg gccagcctac gggcgggtcc ttgggagcga
     9481 ggagaatgga cccatggccg cccttgcatt ccgtgaccct cgactcgaag ccgccgtgga
     9541 gcgggcctgg gcgcgcagtc gactgtcgtt gcggacccgg gtggaccgcc tcgtcgacaa
     9601 gagctggcat gtcgtgcagt gcgccgttgc tgccggggtc gcctggtggt tcgcctcggg
     9661 cgtgttgcac cacgcgatgc cgttcttcgc ccccatcgtc gccgtggtct gcctgggcat
     9721 gacctatggg caacggctgc gccgggtcgc ggaggtgacg gtcggcgtcg ccgtcggcat
     9781 cgccgtcgcc gacatcttcg tgggggtcgt cggtagcggc ccgtggcaga tcaccgtcgt
     9841 cgtgctcgtg tcgatgtcgc tggcgctgct gctggacgcg gggttgttgc tcgtcatgca
     9901 atctgccgtg cagtcaatcg ttgtcaccgt tctcgtcccc acgggttcgc aggcgttggg
     9961 ccgatgggtc gatgccgtga tcggaggagt ggtcgccctc gtcttcgcca tggtcgtgcc
    10021 ccgagcgccg ctgcgtcgcc ctcgccgtca ggccgcccgg gtcggccggg tgatggcccg
    10081 gctgctgcgt gaggcggccc agagcgcgcg ggagggagat gtcgagcgct cggcgcaggt
    10141 gctcgccgac gcccgacaga ccgatccgct catccgcgag ttgcaggccg ccgccgacga
    10201 gggaatgtca gtcatcgcct cgactccgtt cacccgcacg gacgccggca gcgtgcgcag
    10261 catcgcctct gtcgtcgacc cgttggaccg ggccatgcgc agcacccggg tgttggtgcg
    10321 tcggatgagc atcgcggccg acagcgacat cgaggtgccg gacgactacg tggacgtcat
    10381 cgtcgcgttg gccgatgccg tcgacatcct cgaccgggtc tgggcggaga accgctccgc
    10441 ggagttcgcc cggccaggtt tcgttgcgct ggccgaacgg accgcgacgc tggatccggg
    10501 ggagtaccac acgacggtga tcctcgcgca gttgcgctcg ctcgtcgtgg acctgctgga
    10561 gctcagcggc atggaccacg aggacgcagt ggctgccgtc ccaccgcttg gatgaggcta
    10621 gtgacacttg tatgagggtg cagcctccta caagtgacat caacctcata caagcgggtg
    10681 atgtgcccgt ggggggtgag gcggtcagcg gccgaggcgc gcgtagacct gctcggtgct
    10741 ttccttggcc gtgcgccacc aggcttcgtt ggcggcatac cagtcgatgg tctggcgcag
    10801 ccccgcgtgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng acgccgtcga
    10921 ggatgccggt gatctggcgg gggatgaact tttcgacgtg ctgacgtggc ccgtagttgt
    10981 tggagcagtt cgagagggtc gcggcgatct tgaaggaccg gatccaggcg cggacgagca
    11041 gatcggcgct tgccttgctc gccgaatagg gcgaggaggg gttgaccggg gtggattcgg
    11101 agaaccggtt ggggtcatcg agctcaaggt cgccgtagac ctcgtcggtc gagatgtggt
    11161 gaattcgctt gccgtgcttg cggactgctt cgagcaaacg gtaggtcccg atgatgttgg
    11221 cctccatgaa cggccacggg ttgtccagcg agttgtcgtt gtgtgactcg gcggcgaagt
    11281 gcaccaccag gtcagtgtcg gcaacgaggc ggtcgacgag gtcggcgtcg gcgatcgagc
    11341 cctggacgaa ctcgatctgc ccctcgacgg gggcgaggga ggcccggttg ccggcatatg
    11401 tcagcgcgtc cagaacggtc acctcccagt cgggtcgggt ctcgcgcacg cgcaggacga
    11461 agttggcgcc gatgaacccg gcgccgccgg tgacgaggac cttcatgacg ggatctcctg
    11521 tggggtgtcg cccacggccc gcaggccggg gggttctggg gagctggcgg acgggcccga
    11581 gttgaactcc acctcgagcg cgaatcgatg ggcgagatag cggtggtcgg acagggcaag
    11641 gggtatgccg tccgccccca tcgcgacgcg gcgcaccagc aggagcggca cgccgacggc
    11701 ggtgtgcagc aggtcggcat cggccccgct ggccaggccg gcggtgatcg tctggtgcac
    11761 cgtggcgatg gagtggccat cgcgggtcag gctggcccac aggccggggt cggcggcgtc
    11821 ggccctggac aggcggccgg cgaactgctc ggacacccac tcgtgggcga cgtccaggga
    11881 ttcaccgtcg acccggcggg cggaacgcac gtgcaggact ggggcgccga cggcgatctg
    11941 caggatggtg gccacgccgc tgggagcggg gcggaaggag aagtcggcca cctcgcggca
    12001 taccgaggca ccggactcgg ccaccgcgga cccggcgtgc cggaaggtcc ccagagccag
    12061 tcgctggtgg aaagcggtcc cggtgacgaa ccagccggct ccgtgccggg actgcacgag
    12121 cccctcgtcg cgcagcgtct ccagcgcgcg gcgcacggtg ggccgggaca cgccgtagcg
    12181 ggcgcacaac tcggtctcgg actccaacgc cccgtgaacg ccgacgtcgc ccagggcgac
    12241 ccgctcgcgc aggtcatggg cgatcgttga aaaccgggaa cttgtcattg acttgtaata
    12301 tacacaggtt ctcgaaccgt cggtcccgtg gcaacctacg atgtctggca tgacgcgtct
    12361 cagggggatg gtgttgatcg gggggttggt ccccctgttg tcggggtgcc tcgtggtgag
    12421 tgcgctcaac gccagcaacg ggccggccac cggagcgccc acgtcgatcg ccacgtttga
    12481 cccgttcgcc accggcgccg tgcccaaggc gaccggcgcg acgccgtcgc cctcgccgtc
    12541 ccccacgccg tccacgtcga gcagcccctc cccgaagccg agcgcgtcgc ccccgccacc
    12601 ggcccttcct gcgggagcca agtgggcaac cgtcgacagc gagcaggtgc gattcgccgt
    12661 ggcgtcggcc tgggtcgaga tcgtgcccgc caccatgacg accactgcga gcatcccggc
    12721 ggacgtgaag gcgatcgcca aggcgctggg catctcggcc cgcgagctga tcgaccggtt
    12781 cgacgacatc gagatcgctt acgtcgcggc cccggtcaat cgttacgcca ccaacctgtc
    12841 ggcgaccgtt gccgacgtcg gttcgttgcc cgatgacgac tgggtgaaga cgacctactc
    12901 cgagggcaag gtcaaggtgt tggggagcga acgactggcg acccgggtgg gcgacgcgct
    12961 gcggatcaat ggcacggtca cggtccaggg ggcctcgatc gcgttgacga ccttcatcgt
    13021 cgatgtgggt cggcactgca tcgtcttcga ggtggccgcg cgcaaacgcg ccgatgtcga
    13081 cagcgcgatc gctcggctgg ccagcaccct gcacgagatc tgaggcgagc atggcgttgc
    13141 cgtggtggac cttgttcgtg ctcgcagcgg cggcctgctc gtggatctcg gtgacggtgc
    13201 tcgcccgcaa caacccgggg gagcggctgt cgtggttccg ttcaccgacg gtggctccgc
    13261 tgtcacaact gtggttcctc gcggcctccg tcgcgtgtgc ctggctcggc gggtcgtatg
    13321 ccgccgacac cgcgctcggc ggatgggcct acccgatcgc tgcgcttgcg gtcctggtgc
    13381 cgtgggcgct ggtgcgagcc gggcacaacc ggcacgtggg ctgacccggg gctgggtcag
    13441 gcgcaatccg cctgcgctgc ggggtatcag cggcgcgagt ccggggtctt tgtgggaaag
    13501 tcgccgactc ccgcaggagc ccagatggcc gtcgagcccg cgctgcgtcg tcccgaggtg
    13561 cgtcgggtca tcacctcgca tgccttcgca gcggtcggca tggggctgcc ctggccggtg
    13621 ctgttgcttg ccgtcgagca gtccacgtcg tcccagttgc tgcttggcct ggccggagcc
    13681 gcgaggctgg cgccgtatgt ggccctcagt tggctgtccg ggcgcctggc cgatcggcgc
    13741 gaacgagccc tcatcgtccg gatctccctt tgggcgcgag cggtcgccct gctggtcgct
    13801 gccgccgccc tggctgcctc aaatccgctg gtcgccgtcg tcgccgcgac gggtgcgatc
    13861 gtcgccggca caccggcata ccccgccttg gctgcaggta tgccgcgcct ggccggggcc
    13921 gcgaccgagc gggccaccag tgcgctggtc accgtcgagg tggcctcctt cgtcgtcggg
    13981 cccgccgtcg gcggcctatt cgtcgggcgg atgccgcctg cctgggtccc gctcatcggt
    14041 gcgatcttcg tcggcgtggc gatcgtggcc ttcggacgca ccgccatgcc agcacctgag
    14101 gcgagcgcga tcgacgtggc cgagggcggc gtgtggtccc tcttgcggcg cgacccctgc
    14161 gctcggcagg cgctcgccgt cgttgccgtt gtcaacgcgc tgctcagcgt gctcgggttg
    14221 ttgctgctgc aactcgccca cgaggtgtgg cgatcgggcg acgagggctt cgggctggcg
    14281 actgccctgc tcggcttcgg ggctctcggg gcaccggtcg tcgctcactc gcggacgatg
    14341 cgctccggac gggccgccct actcaccctg gccggcgcgg ctgccgtgtt cgctctcctg
    14401 ccgggcggcc ctgcatcgtt cggtgctctc gccgtcatcg gcgccgtggc cgtgtgttgc
    14461 gagaacgtgg cgaccagcgt cctgcaggcc accgtcccgg acgagttgag ggccagcgtg
    14521 ctcggcctgg gtgacacggt gatggtcggg gcggcactgc tcgccgctgt cgcgaccccg
    14581 tggttgggag tgcatctggg accggcgtcc ttgctgctcc tcgtcgcggc cggcaccgcg
    14641 atgctggccc tggcctggag tgcgggacgg caggggccgg attcagtggc caccctcccc
    14701 ggggggagcg gcgaggagct ccccgtgcac catgacgaag taggcgtccg ggtgccaggc
    14761 gtgtagcgca ccgaggctct tcgctcggat cgaggggtaa gaaccacagt cgtgacggat
    14821 gatcccccga ggacctccgt cacaaactta tccgaagcat aatctttcgt aactacctac
    14881 tccatgccgc ggcgtcggca cccagagcca ccctccccgg caggtgggcg gacggcatac
    14941 cctgccagtg acctgcgacc cacgggatgg tgcctgatga tcctgccttt cggcccccac
    15001 acgccctcga tcgcggcggg cgcctgggtg gcgcccaccg ccatggtgat cggggacgtc
    15061 gtcatcgagg agggggcgag catctggtac ggcgccgtca tccgcgcgga ccgcgcgagg
    15121 atccggatcg gggcgcgctc caacgtccag gacaacgcgg tgttccacgc cgaccccgga
    15181 gccccggtcg atctcggctg tgacgtgacg gtcggtcacg gggcgatcgt ccacggggcg
    15241 acggtgggag acggcagcct tatcgggatg ggggccaccc tgctcaacca ctcggtggtg
    15301 ggcggcgggt cgctcgtggc agccgcggcg ctcgtccgcg aagggcaggt catcccgccc
    15361 ggctcgatgg tcgccggggt gccggccaag gtgctcggcc ccgttccggc gggcttcgac
    15421 gtccacgtgt cgggaagcgt cgccacctac gagacgctgc gtgacgaaca ccgcggggcc
    15481 accccatagg ctgggcccat ggctgacttc gacgtcgttg tccttggtgc cggtccgggt
    15541 gggtacgtcg cggcgatccg cgccgcgcag ctcggtctga aggccgcagt ggtggaactg
    15601 aagtactggg gcggggtctg cctcaacgtc gggtgtatcc cgagcaaggc gctgttgaag
    15661 aacgccgagt tggcgcacgt cctcacccat gagaaggcca agtacggcat cgagggcgac
    15721 gcgacgatgt cctacggccc gacccacgcg cgctcgcggc aggtgtcggc cggcatcatc
    15781 aagggcgtcc acttcctcat gaagaagaac aagatcaccg agatcgacgg ctgggggacg
    15841 ctcaccagcc cgacctcgat ggacgtcaag gcggccgacg gctcggcgcg ctcgatcacc
    15901 ttcgacaacc tcatcatcgc cacggggtcc gtcacgcgga tgatccccgg cgtgcaggtg
    15961 agcgccaacg tcgtcaccta cgaggagcag atcctcgacc ccaaccttcc cgggtcgatc
    16021 atcatcgccg ggtcgggcgc catcggtgtc gaattcgctt atgtcatgcg caacttcggc
    16081 gtcgacgtga cgatcgtcga gttcctcgac cg
//
LOCUS       DAOMZY010000026        16105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_3259_length_16105_cov_6.084050, whole genome shotgun sequence.
ACCESSION   DAOMZY010000026 DAOMZY010000000
VERSION     DAOMZY010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 16105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16105
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3259_length_16105_cov_6.084050"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="PLK64_02795"
     CDS             complement(<1..1018)
                     /locus_tag="PLK64_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPV78699.1"
                     /translation="MTYPDDLPIAARRDDIAAAIRDHQVVVVAGETGSGKTTQLPKIL
                     LELGRGIHGMIGHTQPRRIAARAVGERLAEELGVELGGVVGYQVRFTDQSSRDSLVKV
                     MTDGILLAEMQRDRELRRYDTIIIDEAHERSLNIDFILGYLKQLLPRRPDLKVVITSA
                     TIDPGKFAQHFADAEGNPAPVIEVSGRTYPVEVRYRPLVREGLPAKNGSAGPPIEVDQ
                     VTGICEAVEELWTEASDHDEVAAHDILVFLSGEREIRDAADALAGLRLPGTEIVPLFG
                     RLSAADQHRVFARHEGRRVVLATNVAETSLTVPGIRYVVDTGTARISRYSQRTKVQRL
                     PIEPV"
     gene            complement(1287..2105)
                     /locus_tag="PLK64_02800"
     CDS             complement(1287..2105)
                     /locus_tag="PLK64_02800"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPV78700.1"
                     /translation="MPSSTTCPPTSTPSTHPTSPSTSSSPRPSTSTAAKPTTSTSTSA
                     KPTTSTPTPTTTTSPPTTPAPRTASVVSVLPTSAKVVALTFDGGADSRGAASILATLR
                     REGVPASFFVTGRFVQAYPTVVAQLATIGPVGNHTWDHPHLPRLSDAQVLSQLDRTRS
                     AIIATTGKDPIPFFRFPFGDSDAHARALVAAHGYQAVGWTVDSLGWKGTSGGMTAAKV
                     LDRVVAAARPGEIVLMHLGANPDDNTTLDADALPQIISRLRSLGYSFVTLDSLR"
     gene            complement(2394..3707)
                     /locus_tag="PLK64_02805"
     CDS             complement(2394..3707)
                     /locus_tag="PLK64_02805"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional o-acetylhomoserine/o-acetylserine
                     sulfhydrylase"
                     /protein_id="HPV78701.1"
                     /translation="MSDTWAFETKQVHAGQSVDSTTGARALPIYQTTSYVFNNTEHAA
                     NLFALKEFGNIYTRIMNPTQDAVEQRIAALEGGLAALLVASGQAAETLAILNIAEAGD
                     HIVASPSLYGGTFNLLKYTLPKFGVTVSFVEDPNDLDSWRAAVQPNTKLFFGETIANP
                     KVEILDIEGVAAVAHEAGVPLIVDNTIATPYVIRPLEWGADIVVHSATKYLGGHGTSI
                     GGVIVDGGTFDFGKDPEKFPNYNTPEESYHGLVFARDLGVGGGLGANLAFILKARVQL
                     LRDLGPAISPFNAFLINQGIETLSLRVERHLENTRKVAEFLKAHPQVESVRWASLPDS
                     PEYAKAQKYAPKGSGAVLAFEIVGGLDAGKTFVEALTLHSHVANIGDVRSLVIHPAST
                     THSQGGDADRLAAGVTPGLVRLAVGIEHIDDILADLTQGFAAVAG"
     regulatory      complement(3745..3862)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00634"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class IV; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00634"
     gene            3946..4989
                     /gene="mgrA"
                     /locus_tag="PLK64_02810"
     CDS             3946..4989
                     /gene="mgrA"
                     /locus_tag="PLK64_02810"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013490991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HPV78702.1"
                     /translation="MTYRTDEYRAASDRYDRMPMRRCGRSGLVLPAVSLGLWHNFGDD
                     VPLERQQAILRRAFDLGVTHFDLANNYGPPYGSAERNFGTIFRRDLRPYRDELILSTK
                     AGYDMWPGPYGQGGGSRKYLLASLDQSLQRMGVDYVDIFYSHRFDPDTPLEETMGALD
                     SAVRQGKALYAGISSYSGARTREAVQILRELGTPLLIHQPSYSMLNRWIETDLLDVLG
                     DTGVGSIAFSPLAQGMLTDRYLTGIPADSRAAQGKSLDPALLSEAALTHIRALHEIAS
                     ERGQSLAQMALAWALRDPRVTSVLVGASSVAQVEANVAAVDNLEFSAAQLQAIDAHAV
                     ESGINLWKQSSGD"
     gene            5098..5550
                     /locus_tag="PLK64_02815"
     CDS             5098..5550
                     /locus_tag="PLK64_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019482551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78703.1"
                     /translation="MTAALAAWTRRRWIAALVAAAATTVLMGVPTALIPTPVFGREVP
                     PTAWAWPVLLATSVLSGLLLATYVREPGQAEPATTASRSGTIGGLLSFLAVGCPVCNK
                     LALLALGYSGALQWFAPVQPFLAAVGVVLLAGALRQRLRGQLACPVPA"
     gene            complement(5653..6693)
                     /gene="ppk2"
                     /locus_tag="PLK64_02820"
     CDS             complement(5653..6693)
                     /gene="ppk2"
                     /locus_tag="PLK64_02820"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019175196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HPV78704.1"
                     /translation="MSESNTGTTASEAPPVQQPSETLGQGVTNGELTYSTSTFTPATA
                     TLDELRNRMLLDQESDDAKLRYIPGEPLDPDPIAWKQNYPYDKKLKIAEYEATKRLLQ
                     IELLKLQHHVKEQGLKLVVVFEGRDAAGKGGAIKRFMEHLNPRGARVVALEKPTEHER
                     TQWYFQRYVRHMPHAGEIVLMDRSWYNRAGVERVMGFCTPAEYLEFLRQAPAFEKMLI
                     GSGVNLVKLWFSVGRAEQFRRFERRSIDPVRQWKLSPTDMASLDKWDAYTQAKEAMFF
                     YTDTIDAPWTVVKSNDKKRARLEAMRVILSRIDYPDKDVDVVGVPDPLLVGRPELVFD
                     EGERPVRPHLSV"
     gene            6779..7210
                     /locus_tag="PLK64_02825"
     CDS             6779..7210
                     /locus_tag="PLK64_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HPV78705.1"
                     /translation="MIDGEYAPSPTQWVRDQVAQIEASGDTASVSIHGKFVVLLTMRG
                     RSTGLVRKVPLIRVEEGGRYAVIGSRGGAPDDPKWVANLRANPDITVQDGTAVLPMRA
                     REVHGAERDEWWDRCVAMFSDFAEYATKTTRVFPVFVLEPR"
     gene            7359..8336
                     /locus_tag="PLK64_02830"
     CDS             7359..8336
                     /locus_tag="PLK64_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPV78706.1"
                     /translation="MSTTPSTPATPSIDPISKPPVGRPVGHAGARVTVSERSAFAVDG
                     FIAVFVALVLLGLLAQRIIGLATSHVDEPSPGDLTFIVTMILAAALVLSSVVVIQPGE
                     TKVVQFFGRYVGTARRSGLSMLLPLTTKRSVSVRVNNFETNVLKVNDADGNPVEIAAI
                     IVWQVADTAKAVFAVEAYKDFVKVQAEAALRHVATTHPYDDPDGHGESLRGSTDVVSG
                     ELATEVAERVRVAGVEILEVRISHLAYSPEIAQAMLQRQQASAIIAARSMIVDGAVGM
                     VEMALQRLETKDIVVLDDERKAAMVSNLLVVLCSEAKASPVVNAGSLYH"
     gene            8350..8589
                     /locus_tag="PLK64_02835"
     CDS             8350..8589
                     /locus_tag="PLK64_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78707.1"
                     /translation="MSESPSPGSARRAKPQRHAVLLRLDPAVHAALARWADDELRSVN
                     AQIELLVRRALDEQGRMPREAGPLPRRGRPTTDGP"
     gene            complement(8648..9232)
                     /locus_tag="PLK64_02840"
     CDS             complement(8648..9232)
                     /locus_tag="PLK64_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78708.1"
                     /translation="MRTMLDKLISWTGLVMAVVLLAAGGLLTWASNFVAENVRTQLAD
                     QHITMPSGGAIEDPLIKPYLTQFAGQPMETGEQAKAYADHYILVHMNKSSGGKTYSEV
                     SGEFQKLKATPNADPAAVAKLGDLRESLFMGSTLRGLLLNAYAFGTMGVIAMYAAYAA
                     FAGALMFLVLGLLGLRHAARVAPTTASRKEPALV"
     gene            complement(9385..9822)
                     /locus_tag="PLK64_02845"
     CDS             complement(9385..9822)
                     /locus_tag="PLK64_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78709.1"
                     /translation="MSFQDLPDDWPEHPIIDPAIFIDVVDLMATVADREAGAIYVLVC
                     YPSGQLAQPCVITELDEQAFGNKAALFEPFTRLTDTLDGCALVVIVARPGRRQTVDSD
                     REWHEAAARVCRDAGMPLLAVAVATPQAIWRMDESRGQESAIA"
     gene            complement(10028..13015)
                     /locus_tag="PLK64_02850"
     CDS             complement(10028..13015)
                     /locus_tag="PLK64_02850"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HPV78710.1"
                     /translation="MTETANRSTARKSPRGKGITVERIYTTPGVHPYDEVTWERRDVV
                     QTNWKSGETIFEQRGVEFPDFWSVNAGAIVTTKYFRGAQGTPVREWSLRQLIDRVVLT
                     YAKAGKEHGYFATEDDAEIFEHELTYALLHQVFSFNSPVWFNVGTSSPQQVSACFILS
                     VDDSMDSILNWYKEEGKIFQGGSGAGLNLSRIRASSELLSSGGTASGPVSFMRGADAS
                     AGTIKSGGATRRAAKMVVLDVDHPDIEEFIDTKAREEDKIRALRDAGFDMDLGGKDIV
                     SVQYQNANNSVRVSDEFMRAVEEGASFGLRGRMDNRVIREVDARELFDKIAKAAWECA
                     DPGIQYDDTINDWHTNPETGRITASNPCSEYMSLDNSSCNLASLNLLKFLRDDDTFDA
                     VTFQKVAELVITAMDISICFADFPTESIGVTTRDYRQLGIGYANLGALLMATGHGYDS
                     DGGRALAAGITSLLTGAAYKRSAELAAVVGPYAGYARNAAAHQRVMRKHQAANDSVRT
                     YDVMDREIHEAATKAWDAVVKLGERNGFRNAQASVLAPTGTIGFMMDCDTTGIEPDFS
                     LVKFKKLVGGGSMQIVNLTIPRALRKLGYQQESIEAIVEYIADKGHVIDAPGLKPEHY
                     DIFDCAMGARAISPMGHVRMMAACQPFLSGAISKTVNLPETATVEEIADVYLQGWKSG
                     LKALAVYRDNCKVGQPLSDGGSTAKDAAAAKAAGAAGAAEPVVKVVERVEYRPVRKRL
                     PKRRPSQTISFSVGGAEGYLTAGSYPDDGLGELFVKLGKQGSTLAGVMDSFAIAVSIG
                     LQYGVPLETFVEKFTNLRFEPAGPTDDPDIRMAQSIMDYVFRRLALDYLDFETRSFMG
                     IHTADERARHLETGSYAVPDSSDGDYEDDLEASAQSMVRHSPVAPEKSSSAAAPVAPA
                     ASASAPVAAAKSAEHAGEVISGAGAASAREVGIEIHSSQDLLAKFQGKAADAPMCMTC
                     GTKMRPAGSCYVCEGCGSTSGCS"
     gene            complement(13131..13703)
                     /gene="nrdR"
                     /locus_tag="PLK64_02855"
     CDS             complement(13131..13703)
                     /gene="nrdR"
                     /locus_tag="PLK64_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HPV78711.1"
                     /translation="MHCPFCRHGDSRVIDSRTTDDGTSIRRRRLCPECGRRFTTIETA
                     SLSVVKRSGATEPFSRQKVLVGVRKACQGRPVTEDQLARLAQRVEESVRATGSAEVDA
                     HEVGLAVLEPLRELDEVAYLRFASVYRAFESLEDFEAAITLLRAEREATGREPAHLGG
                     GAAYPSGMPRATEDALAGSGGAADSPAPPH"
     gene            complement(14002..15747)
                     /locus_tag="PLK64_02860"
     CDS             complement(14002..15747)
                     /locus_tag="PLK64_02860"
                     /inference="COORDINATES: protein motif:HMM:NF018018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPV78712.1"
                     /translation="MTAPTIGDVRRARPDALGHLATRVGGHAARLRAAASARATSGPG
                     DTWTGPASSAAGGRHTELMVTARTVADGLDRCAAAIRAAGEQVADAQRTLDQAERAAR
                     AGGTHLLDSGALASTGSALPGSPDTAVVVARLAERARADYGAALFTLRARLTAASGRY
                     AVSPPAQSRAPHWSSASWSPWWSQASPTSISPPPDSPIAASAWFAGLSDIERDEVIRS
                     HPEWVGPRDGLPAWARDRANRLVLESVAQRLMAELAARRDEPTDADQPWRDVLRPQLR
                     SRRVIELIAALDGLRAIKEVLATDDGRRRQLLSLDATGGTLTAAVTSGDLDGAGHVAV
                     FVPGFQADVAGDLASYSRGVEAAADLADRLSRAHGDGRPVVGVTWLGYAAPTVLEVVV
                     PGRSVAGWGPATAGSPALGSLLAGLDAAGRAAHPQEPPRLVLWGHSYGSLVTGLALRD
                     ATVPVAAAVVFGSPGMGVDTVAQLRLPPGQLYVEEALDDVVAATSRFAADPGLWREAT
                     QLSVAQQDLPDGSVGRVAHGHEEYLTAGSTSQWNLAAVAAGTTDLVRRRAPCPPWSPL
                     SQQPPPSCERPMRLG"
     gene            complement(15744..16043)
                     /locus_tag="PLK64_02865"
     CDS             complement(15744..16043)
                     /locus_tag="PLK64_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78713.1"
                     /translation="MYLSVCPESLDQVAASLAARVPELPSPAQVVGDLEAIGACLPAF
                     VNHAPAPSSVAGSGRPSGEPWGRSLRDLAAAWVAHSGALGRSAQSYREVDARPLR"
BASE COUNT         2446 a         5343 c         5676 g         2640 t
ORIGIN      
        1 agaccggctc gatcggcaac cgctgcacct tggtccgctg gctgtagcgc gagatccggg
       61 ccgtgcccgt gtcgacgacg tagcggatgc cggggacggt caacgaggtc tcggcgacgt
      121 tggtggccag gaccacccgg cggccctcat ggcgggcgaa gacccgatgc tggtcggccg
      181 ccgagagccg gccgaagagc gggacgatct cggtgccggg aagccgcagc ccggccaatg
      241 cgtctgcggc gtcacggatc tcgcgttcgc cggagaggaa gacgaggatg tcgtgggccg
      301 cgacctcatc gtggtcggag gcctcggtcc acaactcttc gaccgcctcg cagatgccgg
      361 tcacctgatc cacctcgatg ggtgggcccg cggagccgtt cttggccggc agtccctcgc
      421 gcaccaacgg ccggtagcgc acctcgaccg gataggtccg tccggacacc tcgatgaccg
      481 gcgccgggtt gccctcggcg tcggcgaagt gctgggcgaa cttccccggg tcgatggtcg
      541 ccgacgtgat gacgaccttg aggtccggcc gccgcggcag cagttgcttg aggtagccga
      601 ggatgaagtc gatgttgagg ctgcgctcat gcgcctcgtc gatgatgatc gtgtcgtagc
      661 ggcgcagttc gcggtcccgc tgcatctccg ccagcaggat gccgtcggtc atcaccttga
      721 cgagcgagtc gcggctggac tggtcggtga agcgcacctg atagccgacg actcccccga
      781 gctcgacccc gagctcctcg gccagccgct ccccgactgc gcgggcggcg atccgccgcg
      841 gctgggtgtg gccgatcatg ccgtggatgc cgcgcccgag ttcaagaagg atcttcggca
      901 gctgagtcgt cttgcccgac ccagtctcgc ccgcgacgac gacgacctgg tggtcgcgga
      961 tcgcggcggc gatgtcgtcg cgacgggccg caatgggcag gtcgtcggga taggtgatcg
     1021 tggggaccga ctcgcggcgc cgcgcatacc gctcagcccg tgcctcccga gactcacgcg
     1081 gccctctggt cggccgcgcc tcgcgagttg gctggatctg cctcggctcg cccgacccgt
     1141 cggtatgccg ggtcgccggc ttccgacgcg gccgacgccg acgggcgcgc ggcggctgct
     1201 cgggcggtgg cgtggacacg cccgccagga taggccgaga cgtcccgccg tctcgccgag
     1261 attccccgcg accgacccgg acagcttcag cgcagcgagt cgagggtgac gaacgagtag
     1321 ccgagagacc gcaggcggct gatgatctgc ggcagcgcat cggcatccaa cgtggtgttg
     1381 tcgtcagggt tggcgcccag gtgcatgagc acgatctcac ccggtcgagc cgctgcaaca
     1441 acgcggtcga gaaccttcgc ggccgtcatg ccacctgagg tccctttcca ccccaggctg
     1501 tcgaccgtcc acccgacggc ctgatagccg tgggcggcga ccaaggcgcg ggcgtgggcg
     1561 tcggagtcac cgaacgggaa gcggaagaac gggatcgggt ccttgcccgt cgtcgcgatg
     1621 atggccgacc gggtgcgatc gagttggctg agcacctggg cgtcactgag cctcgggagg
     1681 tgcgggtggt cccacgtgtg gttgccgacc gggccgatcg tcgccaactg ggcgaccacg
     1741 gtcgggtagg cctgcacgaa ccgtccggtg acgaagaagc tcgccggcac gccctcccgg
     1801 cgcagggtcg cgaggatcga cgccgcccct cggctgtccg cgccgccgtc gaaggtcaac
     1861 gcgacgacct tggccgaggt cggcaggacg ctgacgaccg atgcggtccg tggggctggg
     1921 gtcgtcggag gcgacgtcgt cgtcgtcgga gtcggtgttg acgtcgtcgg tttggccgac
     1981 gtcgacgtgg acgttgtcgg cttggccgcg gtcgacgtgg acggcctcgg agaggaggag
     2041 gtggacgggg acgtcgggtg ggtcgagggg gtgctggtgg gcgggcacgt ggtgctcgac
     2101 ggcacgaccg tggccgtggg gacacagggg tcagccgtag ccgtagccgg cgcggtcggg
     2161 ctggtggtcg acgagggcgc cagggttcct gggccagtcg cacagcccgc cacgagtgcc
     2221 atcaacgcgc ccacgagtgc cagccttgcc cgcatcgtcc tcgcctcttt cggctcggtc
     2281 cctcactgtg cagacgtgtg gaacaccagg ccggttgacc gcggacgtgc gggagggcgg
     2341 catacccggt cggtatgccg ccctcccgca gtcgcgaggt tcgcgccgcg ctgtcagccc
     2401 gcgacggccg cgaagccctg cgtgaggtcg gcgaggatgt cgtcgatgtg ctcgatgccc
     2461 acggccaggc ggaccaggcc gggggtcacg cctgcggcca gccggtcggc gtcaccaccc
     2521 tgggagtggg tcgtggaggc cgggtggatg accagcgagc gcacgtcgcc gatgttggcc
     2581 acgtgcgagt gcagggtgag ggcctcgacg aacgtcttgc ccgcgtccag gccgccgacg
     2641 atctcgaagg cgaggacggc gccagagccc ttgggggcgt acttctgggc cttggcgtat
     2701 tccgggctgt ccggcaacga ggcccaccgc accgactcga cctgcgggtg cgccttgagg
     2761 aactcggcga ccttccgggt gttctccagg tgccgctcaa cccgcagcga gagagtctcg
     2821 atgccctggt tgatcaggaa ggcgttgaac ggcgagatgg ccgggccgag gtcgcgcagc
     2881 agttgcacgc gggccttgag gatgaaggcg aggttggcgc ccagcccccc accgacgccg
     2941 aggtcgcggg cgaagaccag cccgtgatag ctctcctcgg gcgtgttgta gttggggaac
     3001 ttctccgggt ccttgccgaa gtcgaacgtg ccaccgtcga cgatgacgcc accgatcgac
     3061 gtgccgtggc caccgaggta cttcgtcgcc gagtgcacaa cgatgtcggc gccccactcc
     3121 agcggacgga tgacgtaggg cgtggcaatg gtgttgtcga cgatgagcgg cacgccggcc
     3181 tcatgggcga ccgccgcgac gccctcgatg tcgaggatct cgaccttggg gttggcgatg
     3241 gtctcgccga agaacagctt tgtgttcggc tgaaccgccg cgcgccagga gtcgaggtcg
     3301 ttggggtcct cgacgaagga caccgtgacc ccaaacttcg ggagggtgta cttcagcagg
     3361 ttgaacgtgc cgccgtagag cgacggggag gcgacgatgt ggtcgccggc ctcggcgatg
     3421 ttgaggatgg ccagggtctc agcggcctgc cctgatgcga cgagcagtgc ggcgaggcca
     3481 ccttcgaggg cggcgattcg ctgctcgacc gcgtcctggg tggggttcat gatgcgcgtg
     3541 tagatgttgc cgaattcctt gagcgcgaaa aggtttgcgg cgtgctcggt gttgttgaac
     3601 acgtaggacg tcgtctggta gatcggcagg gcccgcgccc cggtcgtgct gtcgacggac
     3661 tgtccggcgt ggacctgctt ggtttcgaat gcccaggtgt cgctcatcgc tgaatgctct
     3721 ttcgtgccag ggagtcgcca ggcgaccccg cgcttgccga cggcccggtt gggcgccagc
     3781 ctggtcttca cccggggcac cccatcgcgg atggaagggt tgccggtcag cgagccgggg
     3841 cttgtcgctg acactcatga cctagttgga agggtaaaca ggggccgcat tctgggacag
     3901 ggacggctca ccatgtggac cgcccggacc tgcgagcatg tccgcatgac ctaccgcacc
     3961 gatgagtacc gagccgccag cgatcgctac gaccggatgc cgatgcgccg ctgtggccgc
     4021 agcggcctcg tcctgccggc ggtctcgctg ggcttgtggc acaactttgg tgacgacgtc
     4081 ccgctggagc gccagcaggc gatcctgcgc cgagccttcg acctgggtgt cacgcacttc
     4141 gacctggcca acaactacgg gccgccctac ggcagcgccg agcgcaactt tggcacgatc
     4201 ttccggcgcg atctgcggcc ctaccgggac gagttgatcc tgtcgaccaa agccggctat
     4261 gacatgtggc cggggcccta cgggcagggc ggcggctccc gcaaatacct cctggcctcg
     4321 ctggatcaat cgctgcagcg gatgggggtc gactacgtcg acatcttcta cagccaccgc
     4381 ttcgaccccg acactccgct ggaggagacc atgggcgcgt tggacagcgc ggtgcgtcag
     4441 ggcaaggcct tgtatgccgg tatctccagc tactccggtg cgcgcacccg agaggcggtc
     4501 cagatcctgc gcgagttggg cactcccctg ctgattcacc agccgtccta ctcgatgctc
     4561 aaccggtgga tcgagaccga cctgctcgac gtgctcggcg acaccggcgt cgggagcatc
     4621 gccttctcgc ccttggccca gggcatgctc accgaccgat acctgaccgg cataccggcg
     4681 gattcgcgtg ctgcccaagg gaagtcgctc gacccggcgc tgttgtccga ggccgcgctc
     4741 acccacatcc gggctctgca cgagatagcc tccgagcgtg gtcagagcct ggctcagatg
     4801 gcgttggcct gggcgttgcg tgacccgcgg gtcacctcgg tgttggtcgg cgcctccagc
     4861 gtcgcgcaag tggaagccaa cgtcgccgcg gtggacaacc tggagttcag cgcggcgcaa
     4921 ttgcaagcca ttgacgcaca tgccgtcgag tccgggatca acctgtggaa gcaatcatcg
     4981 ggtgactgag ccgggccccg cgcagtggtt gagaccggtc tcagctcgcc tgcctaggcg
     5041 ggccgccgcg cgtgtgggcg gcggagggcg gcatacccct gccggtatta tcgccggatg
     5101 accgccgccc tggccgcctg gacgcgacgc cgctggatcg ctgccctggt ggcagccgcc
     5161 gcgacgaccg tcctcatggg cgtgccgacc gcgctgatcc cgacgcccgt cttcggccgc
     5221 gaggtgccac cgacggcctg ggcctggccg gtcctgctgg ccacgagcgt gctcagcggt
     5281 ctgctgttgg ccacctatgt ccgtgagccc ggccaggccg agccggcgac gacggcaagc
     5341 cggtcgggca ccatcggcgg cctgttgtcc ttcctcgccg tcggctgtcc ggtgtgcaac
     5401 aaactcgcgc ttctcgcgct cggttacagc ggtgcgctgc agtggttcgc gccggtccag
     5461 ccgttcctcg cggcggtcgg cgtcgtcctg ctcgcgggcg cgttgcgtca gcgtttgcgc
     5521 ggacagctcg cctgccccgt gcccgcctga gcgcggcagc gaccgaccgg caggcgctga
     5581 cccctcacgc aagtgggcgg catacctctg cgtgaggtat gccgcccgcc ttggtgacgc
     5641 gcgcgatggg gctcagacgc tcaggtgcgg acgaaccggc cgctcgccct cgtcgaagac
     5701 caactcgggg cgaccgacga gcaaggggtc ggggacgccg acgacgtcaa cgtccttgtc
     5761 ggggtagtcg attcgcgaca ggatcacgcg catcgcctca aggcgcgccc gcttcttgtc
     5821 gttggacttc acgaccgtcc agggggcgtc gatggtgtcg gtgtagaaga acatcgcctc
     5881 tttggcctgg gtgtaggcgt cccacttgtc gaggctggcc atgtcggtcg gcgagagctt
     5941 ccactggcgg accgggtcga tgctgcgacg ctcgaaccga cggaactgct cggcgcgccc
     6001 aacggagaac cacaacttga ccaggttgac gcccgagccg atgagcatct tctcgaaggc
     6061 cggagcctgc cgcaggaact cgaggtattc ggccggggtg cagaagccca tgacgcgctc
     6121 gacgcctgcg cggttgtacc aactgcggtc catgaggacg atttcgccgg cgtgcggcat
     6181 gtgccggacg tagcgctgga agtaccactg agtgcgctcg tgctcggtcg gcttctcaag
     6241 ggcgacgacc cgcgcgccac gcgggttgag gtgttccatg aagcgcttga tcgcgccacc
     6301 cttgccggct gcgtcgcggc cttcgaagac gacgacgagt ttgaggccct gctccttgac
     6361 gtggtgctgg agcttgagca actcgatctg caggagccgc ttggtcgcct cgtactcggc
     6421 gatcttgagc ttcttgtcgt acgggtagtt ctgcttccag gcgatcgggt cggggtccaa
     6481 cggttcaccc gggatgtagc gcagtttcgc gtcatcgctc tcctggtcca ggagcatccg
     6541 gttgcgcaac tcgtccaggg tcgcggtcgc cggggtgaat gtcgacgtcg aataggtgag
     6601 ttcgccgttg gtcacccctt ggccgagagt ctcggagggt tgctggacgg gaggtgcttc
     6661 tgaggcagtg gtgcctgtgt tggactcgga catcaacgtc ccttctcgtg ctgcgttgtc
     6721 acgagggtag tgggtgggta ctcgggagga cagcgcgcca catggttgac tgggacacat
     6781 gatcgacggc gaatacgcac ccagcccgac gcagtgggtg cgcgaccaag tggctcagat
     6841 cgaggcgtcc ggagacaccg cctccgtctc catccacggc aagttcgtgg tcctgctcac
     6901 catgcgggga cgctcgaccg ggttggtgcg caaggtcccg ttgattcggg ttgaggaggg
     6961 cggccggtat gccgtcatcg gcagccgtgg gggcgctccg gacgacccca agtgggtggc
     7021 gaacctgcgc gccaaccctg acatcacggt ccaggacggg actgccgtgc tgccgatgcg
     7081 cgcgcgtgag gtgcacgggg ccgagcgcga cgagtggtgg gaccggtgcg tcgccatgtt
     7141 cagcgacttc gccgagtacg caaccaagac cacccgggtc ttcccggtct tcgtgctcga
     7201 accccgttga tccctggggc gatccgctcg gacgcccccg tcacgtggcg gacgtgcgct
     7261 agctgtcggt ggaaccggcc acgtctggac gacgtcacag gaaagtgata tcacttagat
     7321 gtcacatacg tgattctcgt tccccggagg caatcgccat gagcaccacc ccgtccactc
     7381 ctgccactcc cagcatcgac cccatcagca agccacccgt cgggcgtccg gtcggccatg
     7441 ccggagcacg ggtcaccgtg agtgagcgat cggcgttcgc cgtcgacggg ttcatagcgg
     7501 tcttcgtcgc gctggtgctg cttggcctcc tggcccagcg catcatcggg ttggccacct
     7561 cgcatgtcga tgaaccgagc cctggcgacc tgaccttcat cgtgaccatg atcctggcgg
     7621 cggcgctggt gctcagctcg gtggtcgtca tccaacccgg agagaccaag gtcgtgcagt
     7681 tcttcggacg gtatgtcggg acggcgaggc gctccgggtt gtcgatgctt ctgccgctga
     7741 cgaccaagcg cagcgtctcg gtgcgggtca acaacttcga gaccaatgtc ctgaaggtca
     7801 acgacgccga cggcaacccc gtcgagatcg cggccatcat cgtctggcag gttgccgaca
     7861 cggccaaggc ggtcttcgcc gtcgaggcct acaaggactt cgtcaaggtt caggcggagg
     7921 cggcgctgcg gcacgtggcg accacccacc cctacgacga ccccgacggc cacggtgagt
     7981 cgctgcgggg ctcgaccgac gtcgtgtccg gtgaattggc gaccgaagtc gccgagcggg
     8041 tccgggtcgc gggagtcgag atcctcgagg tgcggatcag ccatctggcc tactcccccg
     8101 agatcgcgca agccatgctg cagcggcagc aggcttcggc gatcatcgcg gcccggtcga
     8161 tgatcgtcga cggtgccgtg ggcatggtcg agatggctct gcaacgactg gaaaccaagg
     8221 acatcgtcgt cctggacgat gagcgcaagg cggccatggt gtccaacttg ctcgtcgtcc
     8281 tgtgcagcga ggccaaggcg tcgccggtcg tcaacgccgg gtcgctgtat cactaagccg
     8341 ccgttcacca tgtccgaatc ccccagcccc ggctccgccc gccgggccaa accgcagcgg
     8401 cacgctgtgc tgctgcggtt ggaccctgcc gtgcacgccg cgctggctcg gtgggccgac
     8461 gacgagttgc gcagcgtcaa cgcccagatc gaactcctgg tccgccgcgc actggacgag
     8521 caggggcgta tgccgcgcga ggccggcccg ctcccccgcc gcggccgtcc cacaaccgac
     8581 gggccgtgaa acgcgacgaa gggcgcccgc ggctgtgggg cgcccttcgt ggtggagcgg
     8641 actctcgtca gacgagagcg ggctccttgc gggaagcagt cgtcggggca acccgagcgg
     8701 cgtgccggag gccgaggagc ccgaggacga ggaacatgag cgcgccagca aaggccgcgt
     8761 aggcggcata catggcgatg acgcccatcg ttccgaaggc gtaggcgttg agcaggaggc
     8821 cgcgcagggt cgaacccatg aagagcgact ctcgcaggtc gccgagcttg gcgacggcag
     8881 ccgggtcggc attcggagtg gccttgagct tctggaactc gcccgagacc tcggagtagg
     8941 tcttgccgcc cgaggacttg ttcatgtgaa cgaggatgta gtggtcggcg taggccttgg
     9001 cctgctcacc ggtctccatc ggctggccgg cgaactgggt caggtagggc ttgatgagcg
     9061 ggtcttcgat agcaccgccg gacggcatcg tgatgtgctg gtcggcgagc tgcgtgcgga
     9121 cgttctcggc gacgaagttg ctggcccagg tgaggaggcc gcctgctgcg agcaggacga
     9181 cggccatgac caggccggtc caggaaatca acttgtcgag catggtgcgc atgtggatcg
     9241 tgtccttctc gttccggcgg ggccggagtc gattattggt tgacccctca agcgtctccc
     9301 catactgggc ttattagcag aggccgatgg tcccacgtca ctgagtcatg agtcacagga
     9361 cgcctgcaga gttgccttgt cgcgctaggc aatcgcgctc tcttgacccc tactctcgtc
     9421 catgcgccag atcgcctgag gcgtcgcgac cgcaacggcc aacagcggca tacccgcgtc
     9481 tcggcagacc ctcgccgcag cctcgtgcca ctcgcggtcc gagtcgacgg tctggcgtcg
     9541 cccgggccgg gccacgatga ccacgagggc gcacccatcg agggtgtcgg tcaggcgcgt
     9601 gaacggttcg aagagggcag ccttgttgcc gaaggcctgc tcgtccaact cggtgatcac
     9661 gcacggctgg gccagctggc cggaggggta gcagaccagg acgtagatgg cccccgcctc
     9721 tctgtcggcg acggtcgcca tgagatcgac gacatcgatg aagatcgcag ggtcgatgat
     9781 ggggtgttcc ggccagtcgt ccgggaggtc ttggaaactc atgcccccac tgtggccgcg
     9841 gcgggcacca gcctgcagaa gttttccaca gcctgccctc gcggtcgatg gcggtccaca
     9901 caggacagcg ggtggggccg ggcttcccct cgggccccac ccgctgtgtc tatgcgtggc
     9961 gtcgggcccg gagtcttacg cccgggcccg actcattcag ttgtggttcc gctccaacgg
    10021 ctggcgttca gctgcagccg ctggtgctgc cgcagccttc gcagacgtag cacgagccgg
    10081 cggggcgcat cttggtcccg caggtcatgc acatcggcgc gtcggccgcc ttgccctgga
    10141 acttggcgag caggtcctgc gacgagtgga tctcgatgcc gacctcccgg gccgaggccg
    10201 ctccggcgcc ggagatgacc tcgccagcgt gttcggcgga cttggctgcg gcgaccggtg
    10261 ctgacgcgga ggccgcggga gccaccggtg cggcagcgct cgaggacttc tcgggagcca
    10321 ccggcgagtg ccggaccatc gactgtgccg aggcttcgag gtcatcctca tagtcgccat
    10381 cggaggagtc ggggacggca tacgagccgg tctccaggtg ccgagcacgc tcgtcggcgg
    10441 tgtggatgcc catgaacgag cgggtctcga agtcgaggta gtccagcgcg agacgacgga
    10501 agacgtagtc catgatcgac tgcgccatcc ggatgtccgg gtcgtcggtc ggtccggccg
    10561 gctcgaagcg caggttggtg aacttctcga cgaaggtctc caacggcacg ccgtactgca
    10621 ggccgatcga cacggcaatg gcgaacgagt ccatcacccc ggccagggtc gacccctgct
    10681 tgccgagctt gacgaacaac tcaccgagac cgtcgtcggg gtaggaccca gccgtcaggt
    10741 aaccctcggc gccaccgacg gagaacgaga tggtctggct cgggcgacgc ttgggcaggc
    10801 gcttgcgcac cgggcggtat tccacccgct cgacgacctt gacgaccggc tccgccgcgc
    10861 ctgccgcacc ggcagccttg gccgcagctg catccttggc ggtcgacccg ccgtcggaga
    10921 ggggctggcc gaccttgcag ttgtcgcggt agaccgccaa cgccttgagg ccggacttcc
    10981 atccctgcag gtagacgtcg gcgatctcct cgaccgtggc cgtctcaggc aggttgaccg
    11041 tcttggagat ggcgccggag aggaacggct ggcacgccgc catcatccgc acgtgaccca
    11101 tcggcgagat cgcccgggca cccatcgcgc agtcgaagat gtcgtagtgc tccggcttaa
    11161 ggcccggggc gtcgatgacg tgacccttgt cggcgatgta ttccacgatc gcctcgatcg
    11221 actcctgctg gtaacccaac ttccgcaggg ctcgcgggat ggtgaggttg acgatctgca
    11281 tcgacccgcc accgacgagc ttcttgaact tgaccagcga gaagtccggc tcgatgccgg
    11341 tcgtgtcgca gtccatcatg aagccgatgg tcccggtcgg cgcgagcacg gaggcctgcg
    11401 cgttgcggaa gccgttgcgc tcacccagct tgacgaccgc gtcccatgcc ttggtggccg
    11461 cttcgtggat ctcacggtcc atcacgtcat aggtgcggac cgagtcgttg gccgcctggt
    11521 gcttgcgcat gacgcgctgg tgggccgcgg cgttgcgggc gtaaccggca tacggaccga
    11581 ccactgcggc gagctcggcg gatcgcttgt atgccgctcc ggtgagcaag ctggtgatgc
    11641 cggccgcgag cgcgcggcca ccgtcggagt catagccgtg gccggtcgcc atgagcagcg
    11701 cgccgaggtt ggcgtagccg atgcccagct gccggtagtc gcgcgtggtg accccgatcg
    11761 actcggtcgg gaagtcggcg aagcagatgg agatgtccat cgccgtgatg accagctcgg
    11821 cgaccttctg gaaggtcacc gcgtcgaagg tgtcgtcgtc gcgcagaaac ttgagcaggt
    11881 tgagcgaggc caggttgcac gaggagttgt ccagcgacat gtactccgag cacgggttgg
    11941 acgcggtgat ccggccggtc tcggggttgg tgtgccagtc gttgatcgtg tcgtcgtact
    12001 ggatgcccgg gtcggcgcac tcccacgcgg ccttggcgat cttgtcgaac agctcgcggg
    12061 cgtcgacctc acggatgacc cggttgtcca tccgcccgcg cagcccgaag gacgcgccct
    12121 cctcgacggc gcgcatgaac tcgtccgaga cccggacgga gttgttggcg ttctgatact
    12181 gcacggacac gatgtccttg ccgcccaggt ccatgtcgaa accggcgtcc cgcagcgcgc
    12241 ggatcttgtc ttcttcgcgc gccttggtgt cgatgaactc ttcgatgtcg gggtggtcga
    12301 cgtccaggac gaccatcttg gccgcgcggc gggtcgcccc accggacttg atcgtccctg
    12361 cggacgcgtc ggcgccgcgc atgaaggaca ccgggcccga ggccgtgcca ccggaggaga
    12421 gcagctcact ggaggcgcgg atccggctca ggttgagccc ggcaccggac ccgccctgga
    12481 agatcttccc ctcctccttg taccaattga ggatcgagtc catcgagtca tcgaccgaga
    12541 ggatgaagca cgccgacacc tgctgtggcg aggaggtccc gacgttgaac cacaccgggg
    12601 agttgaacga gaacacctgg tgcagcagcg cataggtcag ctcgtgctcg aagatctcgg
    12661 cgtcatcctc ggtcgcgaag tagccgtgct ccttaccggc cttggcgtag gtgaggacga
    12721 cgcggtcgat gagctggcgc agcgaccatt cgcgcaccgg cgtcccctgc gcaccacgga
    12781 agtacttcgt cgtgacgatc gcaccggcgt tgaccgacca gaagtcgggg aactcgaccc
    12841 cgcgctgctc gaagatcgtc tcgcccgact tccagttggt ctggacgacg tcgcgccgct
    12901 cccaggtcac ctcgtcgtag gggtgcacac cgggggtggt gtagatgcgc tccacggtga
    12961 tgcccttgcc ccgggggctc ttgcgggcgg tcgaccggtt cgcagtttcc gtcatgactg
    13021 gggtcctccc tgaccttgct gatgcgtctg tctcgtcgtc gtacgtcaag tgcagcaccg
    13081 accaccgaca gccctctcga ttcctcgatc aggcgccggc gccgggtcgt tcagtgcggt
    13141 ggtgctggtg aatccgcggc cccgccagag cccgccaggg cgtcttcggt ggcgcgcggc
    13201 atacctgagg ggtatgccgc cccgccgccg aggtgggccg gctcgcgtcc ggttgcctcg
    13261 cgctcggcgc gcagcagggt gatcgccgcc tcgaagtctt cgaggctctc gaaggcccga
    13321 tagacggagg cgaaacgcag gtaagcgacc tcgtcgagct cgcgcaatgg ctcgagcacg
    13381 gccaggccga cctcgtgggc atcgacctcg gcgctgccgg tggcccggac cgattcctcg
    13441 acccgctgcg cgagccgggc caactggtct tcggtgaccg gacggccctg gcaggccttg
    13501 cggacgccga cgaggacctt ctggcggctg aacggctcgg tcgctcccga ccgcttgacc
    13561 accgacaggc tggcggtctc gatcgtggtg aagcgtcgcc cgcactcggg gcacagccgg
    13621 cgccgcctga tcgacgtgcc gtcgtcggtc gtgcgcgaat cgatgacgcg agagtcgcca
    13681 tggcgacaga acggacagtg catgtgcaga cctccccctc gactcgtgct gaccgtgcgc
    13741 ctcgggcgcg cgcctgcaca caccgctcct gtggacaaac ctggggatcc tcggtggacg
    13801 aggcaggtat gccgtgtgga cagtgtgtgg gtaactcgcc ccagcctgtg gactacatgt
    13861 ggatgaatca cacggctgta actactagat gtaggggtaa ctgtgacctg tcggccctac
    13921 tcctgtcaag cgctggcgag gtttggcacc gatcggcgtg tcgggaccgg cgtgtcacgg
    13981 atcaacctct gctgagcggc gtcaacccaa ccgcatcggc cgctcgcatg agggtggtgg
    14041 ctgctgtgag agcggcgacc acggcggaca gggcgctcga cgacggacca ggtcggtcgt
    14101 gcctgccgcc actgccgcga ggttccactg gctggtcgag cccgcggtga ggtactcctc
    14161 gtggccgtgg gcgacccgtc cgacggagcc gtcgggcaga tcctgttgcg cgacgctcag
    14221 ttgggtggct tcgcgccaca aaccggggtc ggcggcgaat cggctggtgg cggcgacgac
    14281 atcatcgagg gcctcctcga catagagctg tcccggcggc aaccgcagtt gggcgacggt
    14341 gtccacgccc atccccggcg acccgaacac cacggcggca gccaccggca cggtcgcatc
    14401 gcgcagcgcc aggcccgtga cgagcgagcc gtaggagtgg ccccagagga ccagccgcgg
    14461 cggctcctgc ggatgggcag cccggccggc agcatcaagc ccggcgagga ggctgcccag
    14521 agccggagac ccagccgtgg ccgggcccca accggcgacc gaccgcccgg gcacgacgac
    14581 ctcgagaacg gtcggagcgg catacccgag ccaggtgacc ccgacgaccg ggcggccgtc
    14641 tccgtgggcc cgagacaacc gatcggcgag gtcggccgcg gcctcgaccc cgcgggagta
    14701 actcgccagg tcaccggcca catctgcctg gaagccgggg acgaagacgg cgacgtgacc
    14761 ggcaccatcg agatcaccgg acgtgaccgc cgcagtgagg gtgcccccgg tggcgtccag
    14821 gctcagcagt tggcggcgcc gcccgtcgtc ggtggccagg acctccttga tcgcccgcaa
    14881 cccgtcgagg gcggcgatga gctcgatgac gcgccgcgat cgcagctggg ggcgcaggac
    14941 gtcccgccac ggttggtcgg catcggtcgg ttcgtcccgg cgcgcggcca actcagccat
    15001 cagccgttgg gcgacactct ccagcaccaa ccggttggcg cgatcgcgag cccaggcggg
    15061 gagcccgtcc cgaggcccca cccactcggg atggctgcgg atcacctcgt cgcgctcgat
    15121 gtcgctcaac ccggcgaacc acgcactggc cgcgatcggg ctatccggag gtggggagat
    15181 cgaggtcggg gacgcctgcg accaccatgg cgaccacgat gccgacgacc agtggggggc
    15241 cctggactgc gccgggggcg aaacggcata cctgccggaa gcggccgtga gccgggcccg
    15301 cagcgtgaac aaggccgctc cgtagtccgc tcgggcgcgc tcggccaggc gcgcgacaac
    15361 cactgcagtg tccggcgacc cagggagcgc cgagccggtg cttgccaggg cgccggagtc
    15421 cagcaggtgg gttcccccgg cgcgggcggc tcgctcggct tgatcgagag tgcgttgagc
    15481 gtcggccacc tgctcgcccg cggcccgaat cgccgcggcg caccgatcga gcccatccgc
    15541 caccgtgcgc gcggtgacca tgagctcggt atgccgccct cccgcagcgc tgctcgccgg
    15601 tcccgtccag gtgtcaccgg ggccggaagt cgcccgggcc gacgccgccg cacgaagtct
    15661 cgcggcatgc ccgcccacgc gcgtcgcgag gtggcccagc gcgtcgggtc gggcgcgacg
    15721 gacatcgccg atggtcggcg ccgtcatcgc agcggcctcg cgtcgacttc gcggtaggac
    15781 tgcgctgacc tgcccagcgc gccggagtga gccacccagg cggcggcgag gtccctcagc
    15841 gaccgacccc agggctcgcc cgacggtcga ccgctacctg cgaccgacga gggcgctggg
    15901 gcgtggttga cgaacgccgg caggcaggcg ccgatggcct ccaggtcgcc aaccacctgc
    15961 gctggggagg gaagttctgg gacgcgtgcg gcgagggacg ccgccacctg gtcgagggat
    16021 tccgggcaca ccgagaggta caccgctggc tccttccgcc atcggctcga ccaccccaac
    16081 ccttgcctgg actgtaggcg aagcg
//
LOCUS       DAOMZY010000027        15992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_3289_length_15992_cov_6.009977, whole genome shotgun sequence.
ACCESSION   DAOMZY010000027 DAOMZY010000000
VERSION     DAOMZY010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 15992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15992
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3289_length_15992_cov_6.009977"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PLK64_02870"
     CDS             <1..200
                     /locus_tag="PLK64_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78714.1"
                     /translation="LVGLPIDVVEPERDIADLMQLAAEHRLTAYDAAYLQVAAARRLP
                     LATLDATLAEAARAAGVDLLG"
     gene            286..1677
                     /locus_tag="PLK64_02875"
     CDS             286..1677
                     /locus_tag="PLK64_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPV78715.1"
                     /translation="MTDVTTEPAAPTTFRVPTDQATVRANDRQHVFHSWSAQGAITPL
                     PIARAQGSRFWDYDGKDYLDFSSGLVHANIGYGHPKLVAAVQEQAGRMATIAPGYAVD
                     VRSEAARLIASKAPDGLEMVFFTNGGAEATENAIRMARLHTGRHKVFAAYRSYHGATA
                     GALTMTGDPRRIPSEPGLPGIIHFWGPYPYRSAFHSTSPEQETERALGHLRDMIMVEG
                     PAAVAAIILETVVGTNGVLVPPPGYLAGVRAICDEFGIVMIADEVMAGFARCGEWFAI
                     QHWGVTPDLITFAKGVNSGYVPIGGVIISEAIAATFRDRVYPGGLTYSGHPLACASAV
                     ASIQIFEEEGIVEHARMLGTDVIGPALTELAQRHPSIGDVRGLGAMWAVELVRNRETR
                     EPLVPYNATGAAAAPMTEFAAACKARGLWPFVHFNRTHVVPPCTTSVEEVREGLAILD
                     EALTVADGYVTTD"
     gene            1734..4145
                     /locus_tag="PLK64_02880"
     CDS             1734..4145
                     /locus_tag="PLK64_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPV78716.1"
                     /translation="MRQDGAVASSSGRRPVSVEVVEPHMADRVRRASDVSWFLVYAVL
                     AALSITLGNLAVGTAGALEQDLALATGGLPRLLLQLFSWVAGVGVLFLPIAVGVDMLL
                     RSRGWQLVQALLAGGLAAGIAMLIKAAILDGQFTQVLAALTRPLNSNGRTNPLDVLVV
                     TLVALVVVANISGRRWLATLAPLVIGSLLVTGFLAGSNTALALLCSFLLGAMVGHAVR
                     FAFGTASTRAPGTAIARELVNAGTPLQTLELVDEYEEGARLYRGLTPAGEIAVHVFDR
                     DTFGLASGRRLLNRLRLRGATARAPSFTLRSALEHRSLQALALRWAGVWAPFPIAVCD
                     VDGATAVLALTRVEGVSLRVLGENLTDAQARAVVRLLVALQEHRIAFRGLTRDNIVIL
                     PDGAAGLLSVGEGDVGGDDIARRADAAQVMVMLALAIGPERAVALAVDEAGSRTVSRT
                     LPLLQALALGRELRGELKARKGLLDELREQVLTLEDSHNQPATIELRRVTVKGLLTLV
                     GGAVAAYIVLPQLAQVDFAEVLSRAQWPWALGSVLAAVLTFIGASLVLTGSVPTKLRF
                     RHTFMTQLSVAFSGLVAPAAIGNLALNTRYLQRAGVPAGAAGASVGLAQVFQFTSYVT
                     LLAVTGVVAGISPQASFQPPAKVVAAIPVVLVVVIGLFAVPRVRRFFRERLMPQLRQV
                     LPQVLAVLQRPRKLAELLGGALLLDVSFVAALYCATYAFGAAPPLAGVAVVYFAGAII
                     GSAVPTPGGLGGIEAAMSYGLTTLGVDSGTAVSSVLLYRLATYWLPIPFGWASINHLT
                     KIDAI"
     gene            complement(4159..4980)
                     /locus_tag="PLK64_02885"
     CDS             complement(4159..4980)
                     /locus_tag="PLK64_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPV78717.1"
                     /translation="MSYVCPNGHDSTAADFCDTCGAKIDAAAQGLVPEAAQEATPEAS
                     APAAPTSLACPNCATENVPESLFCEACGYDFTTGAMPRGSEPEGESESAGEAAPGGES
                     EQLGEPAAGGIPAAVAAPAPTATGAGVPPAIALEWVAEVWIDPDWYAVQEADEPMPSA
                     GLPVVVPLLVRSALIGRVSASKHIVPDIDCTSDSGVSRRHAQLTTDGTRWFVEDLGSS
                     NGTFVGDAGDALPDDPIDVGPKQELGDDDRIYVGAWTRIVVREATDEEKAAAAAG"
     gene            complement(4977..6263)
                     /locus_tag="PLK64_02890"
     CDS             complement(4977..6263)
                     /locus_tag="PLK64_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPV78718.1"
                     /translation="MAQFTTEVFQNEFLPDGGTDVHAIVRVTCSGAGKAGIGGGTGGG
                     EAAEILIVDTSGSMGANGIMQGRYAATVALNEITDGTWFAIIAGNHVGTLVYPAFGAT
                     QMVRMDPRTRQEAIASIQQFRADGGTAMGTWLTKAKEVFLTVPHVVQRHAILLTDGTN
                     EHETPDQLTWAIQGATGFFQCDVRGLGDRWQIDEARRIASALLGTVDLIPSPERMADE
                     FEKLIRASMARGVASVDLRVWAPQGAQVLFVKQVAPNLEDITAKRSQVNPLTAAFPTG
                     AWADETRDYHVAVRLPAKSLGQEQLASRVQLVVRDEVQAQALVKATWSNDEALTTRID
                     DQVARYTGQAELAAAIQDGLAAKAAGDDAGATRKLGRAVQIATATGDEEMTSRLKKVV
                     EIDNAEAGTVRLRKSVEKLDEMALDTASTKTRRIKK"
     gene            complement(6244..7656)
                     /locus_tag="PLK64_02895"
     CDS             complement(6244..7656)
                     /locus_tag="PLK64_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HPV78719.1"
                     /translation="MSAPVAPAAPPAAAGTCPACGAERAADAAFCEECGHDFGADTPT
                     PATAPVGTTGESQTRHAPVRAQTAAAGEESPLDLGWTGPVSRGAAAEVDPPAAPRCAQ
                     CGTGSFLDGYCDTCGAKAPDPRDHFTEEPVAWIGGVCDIGRRHSRNEDAMALMGSARP
                     LTQAVLVVCDGVSNTTDSHIASLAAARAGREVLDDPVPRGVGLPEAVVGTISKRLGEA
                     VVAARAAVLEATHNPADETPPSCTFVAALIDEGLAVVGNVGDSRAYWLPDAPASQPRQ
                     LSQDDSFAAEQMREGMPRKDAETGPNAHAITRWLGIDSPDDLTPHTASLQLVEDGWLM
                     LCSDGLWNYCSDAAALRDLVATTVAGLGSAGLHPPTLAQALVDFANAQGGIDNITVAL
                     ARVGATSGPPESAPPASTQEPPPDAEPAPAAYPASEPVTASATPTAEPAPPSLPAPAP
                     APASSTGADREDSPDGTVHH"
     gene            complement(7653..10094)
                     /locus_tag="PLK64_02900"
     CDS             complement(7653..10094)
                     /locus_tag="PLK64_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPV78720.1"
                     /translation="MTTPAKCQEPGCTGSILDGYCDVCGTPRATSAGAASTGVASPEA
                     AGTGAAAAALAAEEEPITPAARPQAQVASAVPNGTPCAQPGCQGHILDGYCDVCGTAA
                     DSDPAESAADPSPMGSTRSVASNRLSSTAIGSARARQTGTKATRRVGSSSQRMRSARL
                     GAGLTVIPPMPAIDAATAVMQNPVVPEDKRFCSNCGTKVGRSQGDRPGRTTGFCPKCR
                     QAFSFDPKLQPGDLVAGQYDVAGCLAHGGLGWIYLARDRNVSNRWVVLKGLLNTGDPD
                     ALAAAIAEQRFLAQVEHPRIVEIYNFVMHEGAGYIVMEYVGGTSLKALLKQRMRKTGR
                     YDPLPVDQALAYILEILPAFQYLHDLGLAYCDFKPDNVIQVGDDLKLIDLGGVRRMDD
                     DDSAIFGTLGYQAPEVAEQGVSVASDIYTIGRSLVVLTTEFRGYQTTYVDSLPPVEDT
                     PVFARYDSFYRLLLKACAKSPDDRFASADELRVQMLGVLREAVAQDAKVGRATTSATS
                     LLFEEPSISTDRLEWWSLPRLRPDPHDAQAALVRDVRSLEPHERFEPLRAADPATPDV
                     QLELARTALDRGDAGVVDAATNTLLHEDPWEWRAVWIQGLAALARRDTAAAQAAFNAV
                     YGQVPGELAPKLALAFACEVSGEAAIAESLYAVCARTDSNYTPVGAFGMARIRRAEGG
                     IPAAVEALDLIPVTSRAYALARHQRGQLLADSGQGLPALAEALRSVDGVPLDASDKAG
                     LTVQVLENALTWVTSPQGGPNPGITLAGHPASVSGVRQGLEAAYRQLAEVTPDPAART
                     ALVDQANAVRPWSVL"
     gene            complement(10091..11317)
                     /locus_tag="PLK64_02905"
     CDS             complement(10091..11317)
                     /locus_tag="PLK64_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78721.1"
                     /translation="MSLSLDPIPQPAAGGALPGAAAPEAPGRLGTPLGVHEAYAYLRD
                     LGVWRDKRKAELDSLDAAALASTDRDSFSRDMLVSMALWKAISDRYDLLTVTFDSGRV
                     GPTEAARLSTLIWGRLDVAPDSAAATISPTTAGALGLSLPEACRLCDAMTSSLRARLS
                     LEPSGAEVGTRLKDVRATIERIRDQVAIAPAGVDREAARALLTRLEQRLVDVTDRAKR
                     GAEVGGLLAPLELEAAGAERDLIVGAGRRVETARDAERAAQVRDELLARSAAVQQLVD
                     RCVAAVTPAPRLAVPDVRALGPVPSGGAAVDTYLKRLDAVGRALTVVHTAYGDPLAER
                     DEIAGYAGALAAQARATGVSDPDLDHLRERLQTVVDTTPTDLRRARALRDAYQAYAMS
                     LRTTDTSSGRKGASPR"
     gene            complement(11331..12356)
                     /locus_tag="PLK64_02910"
     CDS             complement(11331..12356)
                     /locus_tag="PLK64_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPV78722.1"
                     /translation="MSRMPPTRLPLWAAGTTLLLSLAACGAGGYAQTPLSPTPTAAAT
                     TSAAPTTAAPTPAASKCNPVPAGFNPLQTYDPLPSLPASGALTGRLAEIRARGYLIAG
                     VSADTLLMGARNPLTGQIEGFDIDLVHEIAKAILGDAAKVQLVVITAGQRQSFLVNRT
                     VDIVVRNMTMNCARWLDWPIAFSAEYYHSGQKVLVRKGSTATSLDDLAAAKAKVCAPT
                     GTTSLAKLQEFVAKGVVPVTADTHTGCLLHFQQAKVDAITGDDTVLAGLATQDPYAVV
                     PAQTAITAEPYGVGFNSEDKVFVQYVNRLLETTKADGRWKASYDRWLAPSLGDAPTPP
                     AAVYGRQ"
     gene            complement(12353..13795)
                     /locus_tag="PLK64_02915"
     CDS             complement(12353..13795)
                     /locus_tag="PLK64_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78723.1"
                     /translation="MSQNPQARSRGPAAAAAAVAPPPPSASGPGAETVAPAVAMPAPA
                     AGGVRPAYANSAGAPAKARVGTWVRGKASGTPGRMRLFGALAAIAAIVFGLAGAWNLW
                     SSAQALGRAGATTDQVVRVQSIYADVLRADADASNAFLVGGLEDPAQRADYEAAIKRA
                     ATTITAAASAQPADGVALAALNSALQDYAALVERARAENRQGLPIGVTYMTSASSGLR
                     TTAVPILEALLQANTERSRGELGAASSSWPTLVAGVLAVLVLGLVSIWLARRTHRYLN
                     SGLAGATVAVLVALIFGLVVFNGIGSDAKRVADNQLAGTLALTSARTAAFDARANESL
                     GLIRRGQAGPFETLWKARDAEVTARLKALVGRVSWYGGSGTNASALIAAWAAYEKAHA
                     EVRTLDDGGSWDAAVAKATTATESPRTTFDAFQAASATAVDGFQSALTGDIEGPTTKA
                     RIAALVLLLAGLAAAVLSTRGIAQRVEEYR"
     gene            complement(13792..15357)
                     /locus_tag="PLK64_02920"
     CDS             complement(13792..15357)
                     /locus_tag="PLK64_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPV78724.1"
                     /translation="MSQLREAVDALVAVATEAGLPPDRARDEAMVFAAAIAEAAPRAA
                     QDWCAAIEGAPTELAAVNAAFFDAASRGRKSRGAPTALLSELAATAPGRATAYAKALT
                     EVASAACSLGEPTLRVTGNAAVAAAAQLEAARLADPGATSRGLPPGVPGMPPSPTSEW
                     DRIWPTPTLTPFPWSTPNGIPGTPVTPGTPSQPSSGGDQAQVAGDPVDAAPPAEPEPP
                     KPTKTLEELLADLDAMIGLERVKREVHQQVAMLKVDKLRREAGLKNADITRHLVFVGN
                     PGTGKTTVARMVGGIYQSLGLLSKGQLVEVDRSELVAGYLGQTAIKTAETCAKAYGGV
                     LFIDEAYALSGDQYGQEAVNTLVKEMEDHRDDLVVIVAGYPDPMVAFIAQNPGLSSRF
                     KTVIEFEDYTDDEIVAILHKLATAADYTVTPEAEGHFRSVLAATARNEAFGNGRFARN
                     ALEEAIGRQAWRLHQDADLTSDQLRELRVEDFRPHVDPEPATTDIVAFGDDPADESSE
                     PPEDSTDEGATPS"
     gene            complement(15358..15957)
                     /locus_tag="PLK64_02925"
     CDS             complement(15358..15957)
                     /locus_tag="PLK64_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78725.1"
                     /translation="MPTADDLLAAVDRVEAMAAAGKVRPEVLARLRRVTKTVRETIPR
                     MDRLGGGSTQAYHVMATATDYLPEAIGGYLRLPREWADSRPVDRGKTPLMILVDQLDL
                     LGRTMDRVFDAINRADAEALVIHGRFLQEKFGHASTGGELALGSATPLPTMPTTPTMP
                     PAPTPTATQTPTAPTPPATQMPATGTPQVRLQPPIGRQG"
BASE COUNT         2138 a         5550 c         5853 g         2451 t
ORIGIN      
        1 tgttggtggg gttgccgatc gatgtggttg aaccggagcg cgacatcgct gacctgatgc
       61 aactggcggc cgagcaccgg ctcacggcct atgacgcggc atacctgcag gtcgcggcgg
      121 ctcgccgact gccgctggcc accctggacg ccaccctggc cgaggccgcc cgagcagcgg
      181 gcgtcgacct gctcggctga gcagaaccgc cgccaggacg tcggaatcgc tcacgcgagc
      241 cacagatggg ctgcgccgcc ccggcgcggc atacagtgtg tcgccatgac cgacgtcacg
      301 accgagcccg ccgctcccac caccttccgt gtccccaccg accaggcgac ggtccgcgcc
      361 aacgaccgcc aacacgtctt ccactcgtgg tcggcccagg gcgcgatcac cccgctgccg
      421 atcgcgcggg cgcaggggag tcggttctgg gactacgacg gcaaggacta cctggacttc
      481 tcctcgggcc tggtccacgc caacatcggc tacgggcacc ccaaactcgt tgccgcagtg
      541 caggagcagg ccgggcggat ggccaccatc gcccctgggt atgccgtgga cgtccggtcc
      601 gaggccgctc gcctcatcgc gagcaaggcc cccgacggac tggagatggt cttcttcacc
      661 aacggcggtg ccgaggccac tgagaacgcc atccggatgg ctcggctgca caccgggcgg
      721 cacaaggtct tcgcggcata ccgctcctat cacggggcca ccgcgggagc cctgacgatg
      781 accggcgacc cgcgccgcat tccgagtgag ccggggctgc cggggatcat ccacttctgg
      841 gggccttacc cctaccgcag cgcgttccac tcgaccagcc ccgagcagga gaccgagcgg
      901 gcgctggggc atctgcgcga catgatcatg gtcgagggcc ccgccgcggt ggccgcgatc
      961 atcctcgaga cggtcgtcgg caccaacggc gtgctcgtgc cgccgccggg ttatctcgcc
     1021 ggtgtgcggg cgatctgcga cgagttcggc atcgtcatga tcgccgacga ggtgatggcc
     1081 gggttcgcgc gctgcggcga gtggttcgcg atccagcact ggggcgtgac ccccgacctc
     1141 atcaccttcg ccaagggcgt caactccggc tacgtcccga tcggcggggt catcatcagc
     1201 gaggccatcg ccgccacgtt ccgcgaccgg gtctacccgg gcggcctgac ctactccggc
     1261 cacccgctcg cctgcgcctc ggcggtcgcc tcgatccaga tcttcgagga ggaaggcatc
     1321 gtcgagcacg cccggatgct cggcaccgac gtcatcggcc ccgccctcac cgagctcgcc
     1381 cagcggcacc ccagcatcgg cgacgttcgt gggctcggcg cgatgtgggc ggtcgagttg
     1441 gtccgcaacc gtgagacccg cgagccgctc gtgccctata acgccacggg ggccgcggcg
     1501 gcgccgatga cggagttcgc agcggcctgc aaggcacgcg ggttgtggcc gtttgtccac
     1561 ttcaaccgca cccacgtcgt gccgccctgc acgacgagtg tcgaggaggt ccgcgagggc
     1621 ctggcgatcc tcgatgaggc gttgacggtc gcggacggct acgtcacgac cgactgagtc
     1681 gggcgccaag tcgggcgcca aatcgggcgg ggcgtgcggc ataccctcgc cccgtgcggc
     1741 aggatggggc ggtggccagc agctcgggac ggcgcccggt gagcgtcgaa gtggtcgagc
     1801 cgcacatggc cgaccgcgtt cgccgcgcct ccgacgtctc ctggttcctc gtgtatgccg
     1861 tcctcgcggc cctgtcgatc accctcggca acctcgcggt cggcaccgcg ggcgccctgg
     1921 aacaggacct cgcgctagcg acggggggcc tgccccggct actgctgcaa ctgttctcgt
     1981 gggtcgccgg cgtcggcgtg ctcttcctcc cgatcgccgt cggcgtcgac atgctgctgc
     2041 gctcgcgcgg ctggcaactc gttcaggcgc tgctcgccgg tgggttggca gccggcatcg
     2101 ccatgctcat caaggcggcg attctcgacg gtcagttcac ccaggtcctc gcggcgctca
     2161 ctcgaccgct gaacagcaac gggcgcacca accccctcga cgtcctcgtc gtgacgttgg
     2221 tggcgctcgt cgtcgtcgcc aacatcagcg gccgacgatg gctggccacc ctggcgcccc
     2281 tcgtcatcgg ctcgctgctg gtcaccgggt tcctcgccgg gtcgaacacc gctctcgcgc
     2341 tcctgtgctc gttcctgctg ggcgccatgg tcggccacgc ggtccggttc gcattcggca
     2401 ccgcctcgac cagggcgccg ggcacggcga ttgcccgcga gttggtcaat gccgggacgc
     2461 ccctgcagac ccttgagttg gtcgacgaat acgaagaggg ggcccgcctc tatcgcggcc
     2521 tgactcccgc cggcgagatc gcagttcatg tcttcgaccg ggacaccttc ggcctcgcct
     2581 ccggccgccg cctgctcaac cggctgcggc tgcgcggtgc cacggccaga gcgccgtcct
     2641 tcaccctgcg ctcggccctg gagcaccgca gtctgcaggc cttggccttg cggtgggccg
     2701 gggtctgggc gccgttcccc atcgcggtct gcgacgtcga cggagccacg gcggtcctgg
     2761 ccctgacccg ggtcgagggg gtctcgctgc gggtcctcgg cgagaacctc accgacgccc
     2821 aggctcgcgc cgtcgtgcgg ctgctcgtcg ctctgcagga gcaccgcatc gccttccgcg
     2881 ggctcacccg ggacaacatc gtcatccttc ccgacggggc agcaggcctg ctgtcggtcg
     2941 gcgagggcga tgtcggcggc gacgacatcg ccaggcgagc agacgccgcc caggtcatgg
     3001 tcatgctggc cctggcgatc ggtccggagc gcgccgtcgc tctcgccgtc gacgaggccg
     3061 ggtcgcgtac cgtctctcgc accctgccgc tgctgcaggc cttggcgctc ggacgggagt
     3121 tgcggggcga gctcaaggct cgcaagggtc tgctcgacga gttgcgtgag caggtcctca
     3181 cccttgagga cagccacaac cagcccgcga ccatcgagtt gcgtcgggtc accgtcaagg
     3241 gactgctcac cctcgtgggt ggcgcggtcg cggcatacat cgtcctgccg caactggccc
     3301 aggtggattt cgccgaggtg ttgtcccgcg cccaatggcc gtgggcgctg gggagcgtgc
     3361 tggcggccgt cctgacgttc atcggggcct cgctcgtgct caccggttca gtgccgacca
     3421 aactgcggtt ccggcacacc ttcatgaccc agctgtcggt ggcgttcagc ggcctggtgg
     3481 cgcccgccgc gatcggcaac ctggccctca acacccgcta tctgcagcgt gccggcgtcc
     3541 cggcgggggc tgccggtgcc agcgtcgggt tggcccaggt cttccaattc accagctacg
     3601 tcaccctgct cgccgtgacc ggcgtggtcg ccggaatcag cccgcaggcg tcgttccagc
     3661 cgcccgccaa ggtcgtcgcg gccatccccg tcgttctcgt cgtcgtcatt ggcctcttcg
     3721 ccgtgccgcg ggtgcggcgg ttcttccgcg agcggctcat gccgcaactg cgccaggtcc
     3781 tgccgcaggt gctggctgtg ctccagcgcc cgcgcaagct cgccgaactg ctcggcgggg
     3841 cgctgttgct cgacgtttcc ttcgtcgcgg ccctctactg cgcgacctac gctttcggcg
     3901 cggcgccacc cctggcaggc gtggccgtcg tctacttcgc cggcgcgatc atcggctcgg
     3961 ccgtcccgac ccccggcggc ctcggcggta tcgaagccgc gatgtcctac ggcttgacca
     4021 ccctcggcgt cgacagcggc acggcggtgt catcggtcct gctctaccgc ctggcgacct
     4081 actggttgcc catccccttc gggtgggcat caatcaacca cctgaccaag atcgacgcga
     4141 tctaacaccg accggtgctc accccgcggc ggcggctgcc ttctcctcgt cggtcgcctc
     4201 gcgcacgacg atccgcgtcc aagccccgac atagatccga tcgtcgtcgc cgagctcctg
     4261 cttgggcccg acgtcgattg ggtcgtccgg aagcgcgtcg ccggcgtcgc cgacgaacgt
     4321 gccgttcgag gacccgaggt cttcgacgaa ccaccgcgtg ccatcggtcg tcaactgggc
     4381 gtgccgacgg ctgacgccgc tgtcactcgt gcagtcgatg tccgggacga tgtgcttgct
     4441 cgccgacacg cggccgatga gcgcactgcg cacgagcagc gggacgacga ccggcagccc
     4501 ggcggagggc atcggctcgt cggcctcctg cacggcatac cagtcggggt cgatccacac
     4561 ctcggcgacc cactccagcg cgatggcggg aggcacgccc gcgccagtcg cggtcggcgc
     4621 gggtgcggct accgcagcag gtatgccgcc cgccgcgggc tcgcccaatt gctccgattc
     4681 cccgcctggg gccgcctcgc ccgccgactc cgactcgccc tcgggctccg agccgcgggg
     4741 catggcgccg gtcgtgaagt cgtatccgca ggcctcgcag aacaggctct cggggacgtt
     4801 ttcggtcgcg cagttggggc aggcaaggct ggtgggagca gccggcgccg atgcttcggg
     4861 cgtggcctcc tgggcggcct caggcaccag accctgggcg gcggcgtcga tcttggcccc
     4921 gcacgtgtcg cagaagtccg cggcggtcga gtcgtggccg ttggggcaga cgtagctcac
     4981 ttcttgatcc tcctggtctt ggtggaggcg gtgtccagcg ccatttcgtc gagcttctcg
     5041 acgctcttgc gcagccgcac ggtcccagcc tccgcattgt cgatctcgac gaccttcttc
     5101 agccgcgagg tcatctcctc gtcgcccgtg gccgtcgcga tctgcaccgc ccggcccagt
     5161 ttgcgggtcg cgcccgcgtc gtcgccggcg gccttcgcgg ccagcccgtc ctggatcgcc
     5221 gctgccaact cggcctgacc ggtatagcgc gccacctggt cgtcgatccg agtcgtcagc
     5281 gcctcgtcgt tggaccaggt cgccttgacc agcgcctggg cctgcacctc gtcgcggacg
     5341 accaactgca cccgcgaagc gagctgttcc tggccgaggc tcttggccgg cagccgcacg
     5401 gccacgtgat agtcgcgggt ctcgtcggcc cacgctccgg tcgggaacgc cgccgtcaac
     5461 gggttgacct gcgagcgctt cgcggtgatg tcctcgaggt tgggggcgac ctgcttgacg
     5521 aacagcacct gcgcgccctg aggtgcccag acccgcaggt cgaccgaggc gaccccgcga
     5581 gccatcgacg cccggatgag cttctcgaac tcgtcggcca tccgttcggg cgacgggatg
     5641 aggtcgaccg ttccgagcag cgccgacgcg atccggcggg cctcgtcgat ctgccaccgg
     5701 tcaccgagcc cacgcacgtc gcactggaag aagccggttg cgccctggat ggcccaggtc
     5761 aactggtcgg gggtctcgtg ctcgttggtg ccgtcggtca gcaggatcgc gtgtcgttgc
     5821 accacgtggg ggacggtgag gaagacctcc ttggccttgg tcagccaggt gcccatggcg
     5881 gtcccgccgt cggcgcggaa ctgctggatc gaggcaatcg cctcctggcg ggtccgcggg
     5941 tccatccgca ccatctgggt cgccccgaag gcgggataga ccagcgttcc cacgtggttg
     6001 cccgcaatga tcgcgaacca cgtgccgtcg gtgatctcgt tgagggcgac ggtcgcggca
     6061 taccggccct gcatgatgcc gttggcgccc atcgagccgg acgtgtcgac gatgaggatc
     6121 tcggccgcct cgccgccgcc ggtgccgcca ccgatgccgg cctttccggc gccggagcag
     6181 gtcactcgca cgatggcgtg gacgtcggtg ccgccgtccg gcaggaactc gttctggaag
     6241 acctcagtgg tgaactgtgc catcggggct gtcctctcgg tcggcaccgg tgctcgatgc
     6301 gggtgcgggt gcaggtgcgg gcaaggatgg gggagcgggc tcggcggtcg gcgtggccga
     6361 cgcggtgacc ggctcgcttg cagggtatgc cgcgggagcc ggttccgcgt ccggcggcgg
     6421 ttcctgggtg ctcgccggcg gggctgactc gggcggcccg gaggtggcac cgacccgagc
     6481 cagcgccacg gtgatgttgt cgatgccccc ttgggcattg gcgaagtcga cgagcgcctg
     6541 ggccagcgtc ggcgggtgca accccgcgct gcccagcccg gcgacggtgg tggccacaag
     6601 gtcgcgcaac gccgccgcgt ccgagcagta gttccacaac ccgtccgaac agagcatcag
     6661 ccagccgtcc tcgaccaact gcagcgaggc ggtgtgcggg gtgaggtcat ccggtgagtc
     6721 gatgcccagc cagcgggtga tcgcgtgggc gttggggccg gtctcggcgt ccttgcgcgg
     6781 cataccctcg cgcatctgct cggcggcgaa cgagtcgtcc tggctgagtt gacgcggctg
     6841 ggaggcggga gcgtcgggca accaatacgc ccggctgtca ccgacgttgc ccacgacggc
     6901 caggccctca tcgatgagcg cggccacgaa ggtgcacgac ggcggggtct cgtccgccgg
     6961 gttgtgggtc gcttcgagga ccgctgcccg ggccgcgacg acagcctcgc ccagccgctt
     7021 gctgatggtc ccgacgacgg cctcaggcag gcccacgcca cgtggcaccg ggtcgtcgag
     7081 gacctcgcgg ccggcgcggg cagcggcgag gctggcgatg tgcgagtcgg tcgtgttgga
     7141 caccccgtcg cacacgacga gcacggcctg cgtcagcgga cgagccgacc ccatgagggc
     7201 catcgcgtcc tcgttgcgcg agtggcggcg cccgatgtcg cacaccccgc cgatccaggc
     7261 gaccggctct tcggtgaagt ggtcgcgcgg atcgggtgcc ttggctccgc acgtgtcgca
     7321 gtagccgtcg agaaagctcc ccgtcccaca ctgggcgcac cgcggagccg cgggtgggtc
     7381 gacctcggcc gccgcgcccc gggacaccgg cccggtccag ccgaggtcga ggggcgactc
     7441 ctcacccgcg gccgcggtct gggcccgcac cggcgcgtgc cgggtctgcg actctccggt
     7501 cgtgccgacg ggggccgtag cgggtgtcgg ggtgtcggcg ccgaaatcgt gcccgcactc
     7561 ctcgcagaag gccgcgtcgg cggcccgctc ggcgccgcag gccgggcagg tacccgctgc
     7621 ggccggcggt gcggcgggag cgacgggcgc gctcacagga cgctccacgg ccggacggcg
     7681 ttggcttggt cgacgagggc ggtccgcgcc gccgggtcag gggtcacctc ggcgagttgc
     7741 cggtatgccg cctcgagtcc ctgccgcacg ccgctcacgc tcgcggggtg cccggcgagg
     7801 gtgatgccgg ggttcggccc gccctgcggc gaggtcaccc aggtcagggc gttctccagg
     7861 acctgcacgg tgagcccggc cttgtcgctc gcgtcgagcg gcacgccgtc gacgctgcgc
     7921 agcgcctcgg cgagggcggg caacccctgg ccggagtcgg cgagcaactg accacgctga
     7981 tggcgcgcca aggcataggc ccgtgacgtc accgggatga ggtcgagggc ttcgaccgca
     8041 gccggtatgc cgccctcggc ccgacggatg cgggccatgc cgaacgcgcc gaccggggtg
     8101 tagttgctat cggtccgggc gcacacggca tacagggact cggcgatcgc cgcctccccg
     8161 ctgacctcac aggcgaaggc gagtgcgagt ttgggggcga gctccccggg gacctggccg
     8221 tagacggcgt tgaaggcggc ctgggcggca gcagtgtcgc ggcgggcgag agccgccaag
     8281 ccttggatcc agacggcccg ccactcccag gggtcttcgt gaagaagggt gttggtggcc
     8341 gcgtcgacga ctccggcgtc gccgcgatcg agcgctgtcc gggccaactc gagctgaacg
     8401 tcgggcgtgg ccgggtcagc ggcgcgcaag ggctcgaagc gctcatgcgg ctcgagggac
     8461 cggacgtcgc ggacgagcgc ggcctgggcg tcgtgcgggt ccgggcgcag cctgggaagg
     8521 gaccaccact cgagccggtc ggttgagatc gagggctcct cgaacaacag cgacgtggcc
     8581 gaggtcgtcg ccctaccgac cttggcgtcc tgcgcgacgg cctcccgcaa gaccccgagc
     8641 atctgcaccc gcaactcgtc ggcgctggcg aaccggtcgt cgggcgactt ggcgcacgcc
     8701 ttgagcagca agcggtagaa cgagtcgtaa cgggcgaaga cgggggtgtc ctcgaccggt
     8761 ggcagcgaat cgacgtaggt cgtctgatag ccccggaact cggttgtcag gacgacgagg
     8821 ctgcgcccga tggtgtagat atccgacgcc accgagacgc cctgttcagc cacctcgggg
     8881 gcctgatagc ccagcgtgcc gaagatcgcc gagtcgtcgt cgtccatccg ccggaccccg
     8941 ccgaggtcaa tgagcttgag gtcgtcgccg acctggatga cgttgtcggg cttgaagtcg
     9001 cagtaggcca gccccaggtc atggaggtac tggaaggcgg ggaggatctc gaggatgtag
     9061 gccaacgcct ggtcgaccgg gagggggtcg tagcggcccg tcttgcgcat ccgctgcttg
     9121 agcaacgcct tgagggaggt cccgccgacg tactccatga cgatgtagcc ggcgccctcg
     9181 tgcatgacga agttgtagat ctcgacgatc cgggggtgct cgacctgggc gaggaagcgc
     9241 tgctcggcaa tggcggccgc cagggcgtca gggtcgccgg tgttgagcag gcccttgagg
     9301 accacccagc ggttggagac gttgcggtcg cgggcgagat agatccagcc cagaccaccg
     9361 tgggcgaggc agccggcgac gtcgtactgc ccagccacga gatccccggg ctggagcttg
     9421 gggtcgaagg agaaggcctg gcgacacttg gggcagaaac cggtcgtgcg gccggggcga
     9481 tcaccctggc tgcgcccgac cttggtgccg cagttggagc agaaccgctt gtcctcgggg
     9541 accacgggat tctgcatcac cgccgtcgcc gcatcgatcg ccggcatcgg cgggatgacg
     9601 gtcaggccag cgcccagacg ggccgagcgc atccgctggc tcgacgaccc gactcgacgg
     9661 gtggccttgg tgccggtctg gcgggcccgg gccgagccga tcgccgtcga ggacaaccgg
     9721 ttactcgcca ccgaacgggt cgagcccatc ggggacgggt cggcggccga ctcggcaggg
     9781 tcgctgtcgg cggccgtccc gcagacgtcg cagtagccgt cgaggatgtg tccctggcag
     9841 cccggctggg cgcacggagt gccgttgggg acggccgagg cgacctgagc ctggggccgg
     9901 gccgcgggcg tgatcggctc ttcctcggca gcaagggccg cagctgccgc tcccgtgccc
     9961 gccgcttctg ggctggctac cccggtgctg gctgccccgg cgctggtcgc ccgtggcgtg
    10021 ccgcacacgt cgcagtaacc atcgaggatc gagccggtgc agccgggctc ctggcacttc
    10081 gcaggggtgg tcatcgcggc gatgctccct tccgtccaga ggacgtgtcg gtggtgcgca
    10141 gggacatcgc gtaggcctga taggcgtccc gcaacgcccg cgcgcggcgc aggtcggtgg
    10201 gagtggtgtc gacgacggtc tgaagtcgct cacgcaggtg gtccaggtcg gggtcgctca
    10261 cgcccgtggc acgggcctgc gcggccagcg caccggcata ccccgcgatc tcgtcgcgtt
    10321 ccgcgagcgg atctccgtat gccgtgtgca cgacggtgag tgcgcggccc accgcgtcga
    10381 ggcgtttgag gtaggtgtcg accgcggcgc cgccggaggg caccggcccg agggcacgca
    10441 cgtcggggac cgccagccga ggagcgggcg tgaccgcggc gacgcatcgg tcgacgagtt
    10501 gctggacggc ggcgctgcgg gcgaggagct cgtcgcgcac ctgggcggcc cgctcggcat
    10561 cccgcgcggt ctcgacccga cgtccggcgc cgacgatgag gtcgcgttcg gcgcccgcag
    10621 cctccaactc caggggcgcg aggagtccgc ccacctcggc tccgcgcttg gcccggtcgg
    10681 tgacgtcgac gaggcgctgt tcgagacggg tcagcagcgc gcgggccgcc tcgcggtcga
    10741 ctccggcagg ggcgatggcg acctggtcgc ggatccgttc gatggtggcc cggacgtcct
    10801 tgagccgggt gccgacctcg gcgccggacg gctcaagaga cagtcgcgca cgcaacgacg
    10861 aggtcatcgc gtcgcagagc cggcacgcct cgggcaacga caggcccagg gccccggccg
    10921 tggtggggga gatcgtcgcg gccgccgagt cgggcgcgac gtcgaggcga ccccagatca
    10981 gggtcgagag tcgagccgcc tccgtggggc cgacccggcc agagtcgaac gtgaccgtca
    11041 gcaggtcgta acggtcggag atggccttcc acaacgccat cgacacgagc atgtcgcggc
    11101 tgaaggaatc gcggtcggtg gaggccaacg cggcggcgtc gagcgagtcg agctcggcct
    11161 tgcgcttgtc ccgccacacc ccgaggtcgc gcaggtaggc gtaggcctcg tggaccccca
    11221 acggggtgcc gaggcgcccg ggagcctccg gagccgcggc accggggagc gccccgccgg
    11281 cagccggctg gggaatcggg tcgagggaca ggctcacggg gcggtcaccg tcactgacgc
    11341 ccgtagaccg cagctggcgg cgtgggggcg tcaccgaggg acggggcgag ccaccggtcg
    11401 tagctggcct tccaccgacc gtcggccttc gtggtctcca ggagccggtt gacgtattgg
    11461 acgaacacct tgtcctcgga gttgaaaccc acaccgtagg gctcggcggt gatcgccgtc
    11521 tgggccggga cgacggcata cgggtcctgg gtggcgagcc ccgccaggac ggtgtcgtcg
    11581 ccggtgatcg cgtcgacctt ggcctgctgg aagtgcagga ggcacccggt gtgggtgtcg
    11641 gcggtgaccg ggacgacgcc cttggccacg aactcctgca gcttggccag gctcgtcgtg
    11701 ccggtgggag cgcacacctt cgccttggcg gcggcgagat cgtccaggct ggtcgccgtg
    11761 ctgcccttac ggacgagcac cttctgaccg gagtggtagt actccgcaga gaaggcgatc
    11821 ggccagtcga gccaccgagc gcagttcatc gtcatgttgc gcacgacaat gtcgaccgtg
    11881 cgattgacga ggaagctctg tcgctgcccc gccgtgatga cgacgagctg caccttggcg
    11941 gcgtcgccga ggatcgcctt ggcgatctcg tggacgaggt cgatgtcgaa gccctcgatc
    12001 tggccggtca gtgggttgcg ggcgcccatg agcagggtgt cggccgacac tccagcgatg
    12061 aggtagccac gggcccggat ctccgcgagc cgcccggtga gagcgccaga ggcgggaagc
    12121 gagggcaacg ggtcgtaggt ctgcagcgga ttgaaccccg cgggcaccgg attgcacttt
    12181 gaggcagccg gggtcggggc cgcggtcgtc ggcgcggcac tcgtggtggc ggccgcggtc
    12241 ggcgtcgggc tcagcggggt ctgggcatac ccaccggccc cgcaggcggc gagcgagagc
    12301 agcagggtcg tcccggccgc ccacagcggc aggcgggtgg gcggcatacg gctcatcggt
    12361 actcctccac acgctgggcg atccctcggg tcgagagcac cgcggcagcc aggccggcga
    12421 gcagcaggac gagagcggcg atgcgcgcct tggtggtcgg tccttcgatg tcaccagtga
    12481 gggcgctctg gaacccgtcg acggcggtgg cgctcgccgc ttggaaggcg tcgaaggtgg
    12541 tccggggcga ctccgtcgcg gtggtcgcct tggccacggc tgcgtcccag ctgcccccgt
    12601 cgtccagggt gcgcacctcg gcgtgcgcct tctcgtatgc cgcccaggcg gcgatcagcg
    12661 cgctggcgtt ggtgcctgac ccgccgtacc aactgacccg gcccacgagg gccttcaatc
    12721 gggcggtcac ctcggcgtcg cgggccttcc acagggtttc gaagggtcct gcctggccgc
    12781 ggcggatcag cccgaggctt tcgttggccc gggcgtcgaa ggcggcggtc cgggcgctgg
    12841 tgagggccaa ggttccggcg agctggttgt cggccacgcg cttggcgtcc gaaccgatgc
    12901 cgttgaacac gaccaacccg aagatgagcg cgacgagaac ggcgacggtc gctccggcca
    12961 gcccggagtt gagatagcga tgggtgcgcc gggccagcca gatgctgacg agcccgagga
    13021 cgagcacggc gaggacgccg gcgacgaggg tcggccagga cgagctggct gcgccgagct
    13081 caccgcgcga gcgctcggtg ttggcctgca gcagggcctc caggatcggc acggctgtgg
    13141 tgcgcaaccc ggagcttgcc gaggtcatgt aggtcacgcc gatgggcagc ccctggcggt
    13201 tctccgcgcg ggcccgttcg acgagagcgg cgtagtcctg caaggcactg ttgagggccg
    13261 caagcgcgac accgtcggcc ggctgggcgc tcgccgccgc ggtgatcgtc gtggcagccc
    13321 gcttgatcgc cgcctcgtag tcggcgcgtt gggccgggtc ctccaggccg ccgacgagga
    13381 acgcgttgct cgcgtcggcg tcagcacgca gcacgtcggc atagatcgac tggacccgca
    13441 cgacctggtc ggtggttgcc ccggcccgcc cgagcgcctg ggcgctggac cacaggttcc
    13501 aggcccccgc cagcccgaag acaatcgcgg cgatggcggc gagcgcaccg aacaggcgca
    13561 tccggccggg agtgccgctg gccttcccac gcacccacgt gcccacgcgc gccttggccg
    13621 gtgcccccgc cgagttcgcg tatgccggtc gcacgcctcc cgctgccgga gcgggcatgg
    13681 cgacggcagg agcaactgtc tcggcgccgg ggccgctcgc ggaaggaggt ggcggtgcaa
    13741 cggcggccgc tgccgcggca ggcccgcggg atcgcgcctg agggttttgg ctcatgaggg
    13801 ggtggctcct tcgtccgtgg agtcctcggg tggttcggag ctctcatctg cggggtcgtc
    13861 cccgaaggcg acgatgtcgg tcgtggccgg ctcgggatcg acgtgtgggc ggaagtcctc
    13921 gacccgcagc tcgcgcaatt ggtcggaggt gaggtcggcg tcctgatgca accgccaggc
    13981 ctgtcgcccg atggcttcct ccaaggcgtt gcgggcgaag cgcccgttgc cgaacgcttc
    14041 gttgcgcgcc gtcgcggcca ggaccgaccg gaaatgccct tccgcctcgg gcgtcaccgt
    14101 gtagtcggcc gcagtggcga gtttgtgcag gatcgcgacg atctcgtcgt cggtgtagtc
    14161 ctcgaactcg atgaccgtct tgaaccggct ggacagcccg gggttctggg cgatgaaggc
    14221 gaccatcggg tcgggatagc cggcgacgat gacgacgagg tcatcgcggt ggtcctccat
    14281 ctccttgacg agggtgttga ccgcctcctg gccgtattgg tcaccggaca aggcgtaggc
    14341 ctcgtcgatg aacaagacgc cgccataggc cttggcgcac gtctcggcgg tcttgatcgc
    14401 cgtctggccg aggtagcccg caaccaactc ggagcggtcg acctcgacaa gctgcccctt
    14461 ggacagcaga cccagggatt gatagatccc gccgaccatc cgggccacgg tcgtcttgcc
    14521 ggtgcccggg ttgccgacga acaccaggtg gcgggtgatg tcggcgttct tcaggccggc
    14581 ttcgcggcgc agtttgtcga ccttgagcat ggcgacctgt tggtgcacct cgcgcttgac
    14641 ccgctcgagg ccgatcatcg cgtcgaggtc cgcgagaagc tcctcgaggg tcttggtcgg
    14701 cttcggcggc tcgggttcgg cgggcggcgc cgcatcgacg gggtcgccgg ccacctgggc
    14761 ctggtcaccg cccgaagatg gctggctagg cgtccctggt gtcaccggtg tccctggtat
    14821 gccgttcggc gtcgaccacg ggaaaggcgt cagcgtcggg gtgggccaga tccggtccca
    14881 ctcggaggtg ggggagggcg gcataccggg gacgcctggg ggcaggcccc gggaggtcgc
    14941 gccggggtcg gccaggcggg cggcctccag ttgggcggcg gcggcgacgg cggcattgcc
    15001 cgtgacccgc aacgtcggtt cgccgaggga gcaggccgcc gaggcgacct cggtgagcgc
    15061 cttggcgtat gccgtcgcgc gcccgggagc ggtggcggcg agctcgctga gcaaggccgt
    15121 cggcgcaccg cgggacttgc ggccgcggct ggccgcgtcg aagaaggcgg cgttgaccgc
    15181 agccaactcg gtcggggcgc cctcgatggc agcgcaccag tcctgggccg cgcggggagc
    15241 cgcttcggcg atcgcggcag cgaagaccat cgcctcgtcg cgagcccggt cgggcggcag
    15301 gcccgcctcg gtggcgacgg cgacgagggc gtcgaccgcc tcacgcagct ggctcatcta
    15361 gccctgcctt ccgatggggg gttgcaaccg gacttgtggg gtgccggtgg cgggcatctg
    15421 cgtcgcaggg ggcgtcggtg ccgtgggcgt ctgagtcgca gtgggcgtcg gagccggcgg
    15481 catggtcggc gtggtgggca tcgtcggcag cggcgtcgcg gacccgaggg ccagctcgcc
    15541 tccggtcgac gcgtgcccga acttctcctg caggaagcgg ccgtggatga ccaaggcctc
    15601 ggcgtcggcg cggttgatgg cgtcgaagac ccggtccatc gtccggccga gcaggtcgag
    15661 ctggtcgacg aggatcatca gcggggtctt gccgcggtcg accggccggg agtcggccca
    15721 ctcgcggggc agccggaggt agcccccgat cgcttcgggg aggtaatcgg tcgccgtcgc
    15781 catgacgtga tacgcctggg tgctgccccc gcccagccgg tccatccggg ggatcgtctc
    15841 gcggaccgtc ttggtcaccc ggcgcagccg ggccagcacc tcagggcgga ccttgccggc
    15901 ggcggccatg gcttcgacac ggtccacagc cgccaacagg tcatcggccg tcggcacacg
    15961 cgggatcggc gtgaccggcg cctgctcctc gc
//
LOCUS       DAOMZY010000028        15734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_3359_length_15734_cov_6.232987, whole genome shotgun sequence.
ACCESSION   DAOMZY010000028 DAOMZY010000000
VERSION     DAOMZY010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 15734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15734
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3359_length_15734_cov_6.232987"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /gene="dnaX"
                     /locus_tag="PLK64_02930"
     CDS             complement(<1..499)
                     /gene="dnaX"
                     /locus_tag="PLK64_02930"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPV78726.1"
                     /translation="MSTALYRRYRPETFADVIGQEHVTEPLMQALRTGRVGHAYLFSG
                     PRGCGKTTSARILARCLNCEQGPTPTPCGTCDSCVALARGGSGSVDVIEIDAASHGGV
                     DDARDLRERASYGPAQSRFKVYIIDEAHMVTPQGFNALLKIVEEPPEHVKFVFATTEP
                     EKVIGT"
     gene            complement(509..1933)
                     /locus_tag="PLK64_02935"
     CDS             complement(509..1933)
                     /locus_tag="PLK64_02935"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HPV78727.1"
                     /translation="MADEPTPLTRRELREQREREEHQRAERAPESRRHADPARARRRP
                     LALGWMLHGWGIGILWLGTHLLVGAAFLLRFGANPDRWNALVVGEVYTQFAGVSQLGR
                     LPIERVLAEVPAPAVRLMQGLYAVTGEDRGRFFLAIVALAVVADLVILIALWRASAGG
                     PVAAAYWALAVPLLGPVTYARLDLVVVAVVVLAWLLAPRRPLWAGALLAVATGLGLWP
                     MAVFLPLLALVPRGSRRLAVAGYAVISLAFIAVGFLGGGWDRLASPITRALTRGLSLE
                     SIPGLPTLVDMALSPGRHTASLVNGDMVVRGPWVEALVTGSTALVVVPVVVVIALGVL
                     VLRRREAPASGDARVGRAKRRAATQPTPATALVALAGLLTLLVTARTLSPEALVWTVP
                     FLAVLAARRPGRGPWLALVVLLVTQIDYPYLFQGLTYPAAAAFDVAAAAILVRNVALL
                     ALLVWSLLAAVRELRSSAGVAPGQ"
     gene            complement(2176..2272)
                     /gene="ffs"
                     /locus_tag="PLK64_02940"
     ncRNA           complement(2176..2272)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLK64_02940"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(2310..3647)
                     /locus_tag="PLK64_02945"
     CDS             complement(2310..3647)
                     /locus_tag="PLK64_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV78728.1"
                     /translation="MSDQASDGGSGASRDGGSGGTPTRPRLRDFWTDLPREGKFLLST
                     IIIDFIGTGLVMPFLVVYLHEVRGFPLDHVGVLASIPAVVSLIVLGPTGHVIDRLGAR
                     RVIIASTIASIVADVWLAFATTELAAAGALVVMGFAGGVIWPAIQSFVSAIMPSHIRQ
                     RYFGMSFTLLNLGIGIGGVIGGTVVDVARPETFVVIYLVNAATFLAPLAIYLGPLRHT
                     DPRPQHAPVDEAAPRVSYWRLLRDRRVAALFALTMVSAYVGYAQLATGAVAFARDVGQ
                     VSTQAIGYGFAANTVVIVALQLPVLQRIENRRRTRVLLAMCVVWAAAWLALGLTGVAP
                     GGAAAAVLFAVCCAVFGLGETLLQPTIPAITNDLASDAVRGRYNALMSMGFQAAAIIA
                     PIQAAWLIEHGQGGSYIGLLVAGCVVMAGLALLVERRIPASANGLARRPAPQR"
     gene            3729..3817
                     /locus_tag="PLK64_02950"
     tRNA            3729..3817
                     /locus_tag="PLK64_02950"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3763..3765,aa:Ser,seq:gga)
     gene            complement(3837..4736)
                     /locus_tag="PLK64_02955"
     CDS             complement(3837..4736)
                     /locus_tag="PLK64_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HPV78729.1"
                     /translation="MKDHEPEQSEPDGSDPRVAAYLARLGRRDLLAAPADRATLVALH
                     RAHVERVPYETLQIALGHPTPVDVAASVASVLRGHGGYCFHLNGAFAWLLERLGYAVQ
                     HHRAGVQGARAATPVGADGNHLALTVDVEGQPWLVDVGLGSAVHEPLPLAPGRCRQGP
                     FTYGIRPSEAVPGGWRFDHDPSAQSFVGMDLDIAPCTLAEATTRHTELSTSPESMFVK
                     VLAVQRRDAHGVDLLQSVALTRIDTAGRRKRVVESAADFEGVLRGVFGLDLTGMPREH
                     LAAVWRRQADGYAAYQEKKTARA"
     gene            4964..5509
                     /locus_tag="PLK64_02960"
     CDS             4964..5509
                     /locus_tag="PLK64_02960"
                     /inference="COORDINATES: protein motif:HMM:NF037627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78730.1"
                     /translation="MTTREPEAIDEGVVELVESLEPNPAYVAGARWDVLHANRAAHLL
                     FADFASRPDHERNMLWYYLAEPSARALYVDWEAEAIAQLGHFREDYEKWPGDPRFDDL
                     LERVFAVTPQARTWWEERRGVDLPRNGVKQIRLADGRLVSLRQLVMSTVDDPEIQVVC
                     YFADIDDDGDYGDDMESLLGD"
     gene            5535..6827
                     /locus_tag="PLK64_02965"
     CDS             5535..6827
                     /locus_tag="PLK64_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HPV78731.1"
                     /translation="MSPGRAAYPSGGDYPRLARRLGLGDAVVIGLGSMIGAGVFAAFT
                     PAAAAAGSGLLVGLALAAAVAWANATSSAQLAAQFPESGGTYVYGRECLGESWGFAAG
                     WAFVVGKTASCAAMAITAATYALGGEARAAQVVGLAVVGALTVVLTRGITKTARLARV
                     LLTVTLACLLVTVLVIAAFTREWAAPSAGVGDATAYGILQSAGLLFFAFAGYARIATL
                     GEEVRAPTVTIPRAITRALGLVIALYAVLALLLLATIGADGIAASALPLLTAAQAADV
                     SWAGVFVRVGATVAALGALLGLLSGVGRTVLAMARRGDLPRLLAAVDPGSKVPAVAQL
                     WVAGAVAVLVLLTDLRHAIGFSSFGVLLYYAIANASAWTQTGEHRRWPRWVQAAGLVG
                     CLALAAALPIGSVVVGALVLGVGVFGRVWLQRRGVARG"
     gene            complement(6970..8571)
                     /locus_tag="PLK64_02970"
     CDS             complement(6970..8571)
                     /locus_tag="PLK64_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HPV78732.1"
                     /translation="MPEQIQQIKAGYEFAGGALAFGAAVLDGAAYPEAQVKIPLAVIN
                     RHGLVAGATGTGKTKTLQLMAEQLSQGGVPVFLADVKGDLSGMASPGVSNEKVAARAA
                     DIGEEWVGTAYPTEFYALGGQGKGIPIRAAVSSFGPTLLAKVLGLNETQESSLGLVFH
                     YADSKGLWLDDIKDLRAVVQHLTSEEGKADLKNLGGLSSATAGVILRELITFADQGAD
                     VFFGLPEFDTADLLRLGADGRGIVSMLELPAVQDRPQLFSTFLMWLLADLFHDLPEVG
                     DLDKPKLVFFLDEAHLLFANASKDFLQAITQTVRLIRSKGVGVFFVTQSPKDVPGDVL
                     AQLGNRVQHALRAYTPDDAKALKAAVSTYPKSAYDLEELLTQLGTGEAVITVLSERGA
                     PTPVAWTRMRAPQSLMAPSADAVIDQIVAASPGTGRYAAAQDRESAFEKLTAAASAAD
                     AAAAQAAAAEAQAKADAEAAKAAEAAAKAEAKADRPAARPAREEPTVVEQVVGSSVFK
                     SMLRSAGTVIGREITRSIFGTSRRR"
     gene            8675..8971
                     /locus_tag="PLK64_02975"
     CDS             8675..8971
                     /locus_tag="PLK64_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HPV78733.1"
                     /translation="MIFKGIGEHRPYPDHGLVTTRDWANVAPRQVRLQDLVTTKRTLD
                     LAQLLADDSTFYGDLFAHVVEWRGVLYLEQGLHRALRAALQQRGTLHARVLVLD"
     gene            9023..9544
                     /locus_tag="PLK64_02980"
     CDS             9023..9544
                     /locus_tag="PLK64_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HPV78734.1"
                     /translation="MSITVESASVARARRRKRTAITLLVVIAMLAGAFYYASSYWSKG
                     STKAAAPACTITAGPSGPVYPSQVTVNVYNATSRTGLAATVGRLVKAKGFVLGTVSND
                     PAKKQIPGTAEVRYGSAGERAAALVKTLVAGATLVKDTRADASVDLVLGSGYKEMATS
                     TGTATLATAPPGC"
     gene            complement(9548..10252)
                     /locus_tag="PLK64_02985"
     CDS             complement(9548..10252)
                     /locus_tag="PLK64_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPV78735.1"
                     /translation="MTTPSNARVEPGLSAEASGLMVHSDHAEHTDHPKRSRSVTRDRL
                     LDAAGEVFAERGVAGASVEEVCERAGYTRGAFYSNFATKDELVVALLDREEGLLLERL
                     STALGASLSEPAPLTAIAGRLFDLQPFGAQNYALRAELSLLAVRDPELAAPYLAAQRA
                     CRERFVPFLLEGLAQAGLALTVAPDDALDTLDSLFETSLRSAIIAGDANSRDNLVARM
                     LPVLLGGMTRPLADAD"
     gene            10373..13423
                     /locus_tag="PLK64_02990"
     CDS             10373..13423
                     /locus_tag="PLK64_02990"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPV78736.1"
                     /translation="MSSALYALGHWAVRRRRLVVALWVALIVILGASAGLLQRGMADA
                     FEIPGTQSQKAMDALSSRFPEFAGASGQVVVSAPAGSKVTDPSVTARIEDLTARYAAL
                     PHVSGATSPFNELVKGTVSPDGSAAIIAVQLDLASDDVTDATREPLIALAHEATATGL
                     TTSVGGALFMPTAPEMSITEGLGVVVALIVLVVTLGSAIAAGIPLLTSLLGVAMSMSF
                     ILIATHFTTITSTAPFLALMIGLAVGIDYALFILSRHRDLLAEGVEVEEAAAQATGTA
                     GSAVVFAGATVAIALLALGVAQIPFLSVMGIAAAVGVGCAVVVALTLLPALIGAAGTR
                     LTPKRVADAHHARRHTSWSASWVRLVTKVPALTAILVIGGLGVAALPARDLTLALPNN
                     GTSAPGSTQREAFDTVARAFGPGANAPLVITLDIVATTDPLGVVADIEKMVLATPGIA
                     STSVATPNRGADTGVIVAIPEGSAESQVTKDALTAVRALAPEVERQFGVELAVTGHTA
                     AQVDVASRLSDALLPFGIVVVGLSLVLLMLVFRSLLVPITAAVGYLLSLGASFGAVSA
                     VAHWGWLAAPLGVSQTGPVIAFMPIIVMGVLFGLAMDYQVFLVSSMRSRYVHGDDAKD
                     AVSHGFVDAARVVTAAAIIMVAVFAAFVPKGALYIKPIALGLAVGVFVDAFVVRMTLV
                     PAVMRLLGPAAWWLPRWLDRLLPVLDVEGESLHRRIAADETRTARAAIEGPVPVIEAH
                     GLGVSDPVGTVFADVDLRVPEGGILVVHGAPGGGKTSLLLTLAGRMAFTAGSLDVAGR
                     LLPDQRHGVRKATALAEITGVNDLDPLLSIGDHLTERLAGRGWLPWVGAHARDRATGL
                     LTDLLGYAVSAVPAAHASPSLASTTRVRDLAPLERWVLGVTLALLDDPEILLVDDIDG
                     LRGSDDRAAAWAVLASLAGSQVRHTARTQPARALTVVVSCRDLHEATDALAGAGGIPL
                     VSLALASRSAEADSFVTEHTGDPWTDPVAASFPDPADELSEKVH"
     gene            13423..15528
                     /locus_tag="PLK64_02995"
     CDS             13423..15528
                     /locus_tag="PLK64_02995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HPV78737.1"
                     /translation="MSRPALPAAELRRFAGSPRALLALFALVLVPLVYGGLYTWANES
                     PTTRVDQIAAAVVNLDEPVTLKGADGKDQIVPLGRVVVGKLTSSASSSNYAWTLTDAA
                     TAESGLKEGRFGAVLTIPADFSRSATSTNGAPADARAARLSLRTDDSVNYLDGAIAKA
                     IATTTSAEAARTVTEGYLSAMYAGYGTLHDQLSSAADGAGKLASGVTELTSGADKAAV
                     GAGSLASGVDQIASGAGDLASGADKLATGADQAAAGVGQLAGGLSQIDSKVAAMPAGT
                     RALADGAAQVSAGLTGLSSGAGQTASGAAALATGLTAYTSGVDGLAATCAASGAAPAF
                     CANLSGLAAQGGGLRDGATGVSGGTAQLSGGLSQLVPGATQLAGGVDQLADGMPALAA
                     GVHQSATGAATLASKLPQLATGVRGVADGADQLASGATTAATGADELTSGLQALSDGS
                     AKLGTGASDLSTGLGEGVSKIPTYSEAERAQLAAVAATPVVADVARDNAVKNNGAGLA
                     PYFMALALWVGSLAIYLLLRPLSPRALASGAPSPLVALAGYLPGALLGAAQGAALALV
                     LQFWIGIGAASTWGLIGVGMLIGLTFVAMNQALVGLLGAPGRFVAVILAGLQLASAGG
                     TYPVATAPGFFGWLHDVLPLTYAVDALRRVVAGATDGLGTDITALVLTLVGSLLVTMY
                     AAHRQRTWTVRRLRPVTVA"
BASE COUNT         2284 a         5575 c         5502 g         2373 t
ORIGIN      
        1 tcgtgccgat gaccttctcg ggctcggtcg tcgcgaagac gaacttcacg tgctccggcg
       61 gctcctcgac gatcttcagc agggcgttga agccctgcgg cgtgaccatg tgggcctcgt
      121 cgatgatgta gaccttgaac cggctctggg ccgggccgta ggacgcccgc tcgcgcagat
      181 cgcgcgcatc gtcgacgccg ccgtggctag ccgcgtcgat ctcgatgacg tcgaccgacc
      241 cactgccgcc ccgcgcgagt gcgacgcacg agtcgcacgt gccgcaaggc gtcggggtcg
      301 gaccctgctc gcagttgagg cagcgcgcga ggatccgggc gctggtcgtc ttgccacacc
      361 cccgcgggcc gctgaagagg taggcgtgac cgacccggcc ggtgcgcagt gcctgcatga
      421 gcggctcggt cacgtgctcc tgcccgatga cgtcggcgaa cgtctcgggc cggtagcggc
      481 gatagagggc ggtgctcacg gtccgaccct actggcccgg ggcgacaccc gcgctagacc
      541 gcagttcacg cacggcggcg agcagcgacc agacaagtag agccagcagc gcaacgttgc
      601 ggacgaggat cgcggcggct gcgacgtcga aggctgcggc cgcaggatag gtgaggccct
      661 ggaagagata cgggtagtcg atctgggtca ccaggagcac gaccaaggcc aaccaggggc
      721 cgcgtcccgg ccgtcgagcg gccaggaccg cgaggaaggg caccgtccac accagcgctt
      781 cggggctgag cgtccgggcc gtgacgagca acgtcagcag cccggcgagc gcgaccagcg
      841 cggtggccgg agtcggctgg gtcgcggccc ggcgcttggc ccggccgacg cgggcgtcac
      901 cgctggcggg tgcctccctg cgccgaagga cgaggacccc cagggcgatc accacgacga
      961 ccggaaccac gaccagcgcg gtggacccgg tcacgagagc ctcgacccag ggcccgcgca
     1021 ccaccatgtc gccgttgacg agcgacgcgg tatgccgccc agggctcagc gccatgtcga
     1081 cgagggtcgg caggccgggg atcgactcca ggctcaagcc acgggtgagt gcccgggtga
     1141 tgggcgaggc caggcggtcc cacccgccgc ccaggaaacc gacggcgatg aacgccaggc
     1201 tgatcacggc atacccagcg acggccaggc gtcgagagcc tcggggaacg agcgcgagga
     1261 gtggcaagaa gaccgccatc ggccagagcc cgaggccggt cgcgacggcg agcagcgccc
     1321 cggcccagag cggacgtcgc ggggcgagaa gccaggccag caccacgacg gcgacgacca
     1381 cgaggtcaag tcgagcgtag gtcaccgggc cgagcagcgg caccgccagc gcccagtagg
     1441 cggccgcgac cggtccaccg gcactggccc gccaaagagc gatgaggatg acgaggtcgg
     1501 cgacgaccgc caaggcgacg atggccagga agaaccggcc gcggtcctcg ccggtcacgg
     1561 catacaggcc ctgcattagc cgaacagcgg gcgcggggac ctctgcgagc accctctcga
     1621 tggggagccg gccgagttgg gacacgccgg cgaactgcgt gtagacctcc cccaccacga
     1681 gggcgttcca ccggtcgggg ttcgccccga agcgcaggag gaacgccgcg ccgaccagca
     1741 gatgggtgcc gagccacagg atgccgatgc cccacccgtg gagcatccag ccgagagcga
     1801 ggggcctgcg ccgggcccgc gcggggtcgg cgtggcgacg ggactcaggg gctcgctcag
     1861 cccgctgatg ttcctcgcgc tcgcgttgtt cgcgcagttc cctgcgggtg agtggggtgg
     1921 gttcgtccgc catgtttcgc cttaggtgat cgaccgcccc cgcggccttc gggtgggccc
     1981 cgaccagtcg agtatgccgt gtgctcgccg caggataccg agttgccgac tgtcgggttg
     2041 gcgcgccaac ggatgaccgc tcattgcggg tgagcagcga cagcactagt gcgaggcggc
     2101 cgtgtggtcg ccctcgcgag ggcccgccgg cagctccgcc ggcgggctcg cccacgcctg
     2161 gggaacatcc gcagacacaa gagcacccca cgtacctgga agagctcacc tgcccttgct
     2221 gcattcctgc cctgggggag ttcagtgagg taccaccacg cggggtgccg tcgcccagtg
     2281 tatgccgtgc ggcgtggccc gcgcgcccct caccgctgcg gggcggggcg gcgagccagc
     2341 ccattggccg acgccgggat gcggcgttcg acgagcaacg ccaagccggc catgaccacg
     2401 cagcccgcga cgagcaggcc gatgtaactg ccgccttgac cgtgctcgat caaccaggcc
     2461 gcctgaatgg gggcgatgat ggccgcggcc tggaagccca tcgacatcaa cgcgttgtag
     2521 cgcccgcgga cggcgtcact ggccaggtcg ttggtgatgg ccgggatcgt cggctgcaga
     2581 agcgtctcgc ctaggccgaa caccgcacaa cacaccgcga agagcaccgc ggcagcggcg
     2641 ccgcccggcg cgaccccggt cagcccgagc gccagccagg cggccgccca gaccacgcac
     2701 atggctagca gcacccgggt gcgacgacgg ttctcgatgc gctgcaggac cggcagttgc
     2761 agggccacga tgacgaccgt gttggcagcg aagccgtagc cgatggcctg ggtggacacc
     2821 tgtcccacgt cgcgagcaaa ggccactgca cccgtggcga gttgggcata gccgacgtag
     2881 gccgagacca tcgtcagggc gaagagcgcg gccacccgcc ggtcgcgcag cagtcgccag
     2941 taggagacgc gcggagcagc ctcatcgacc ggcgcgtgct ggggccgagg gtcggtatgc
     3001 cgcaacggcc cgaggtaaat cgccagcggg gcgaggaacg tggccgcgtt gacgagatag
     3061 atcaccacga aggtctcggg gcgagcgacg tcgaccacgg tgcccccgat gacgcctccg
     3121 atcccgatgc cgaggttgag cagggtgaag ctcatcccga aataccgctg gcggatgtgt
     3181 gacggcatga tcgcgctgac gaacgactgg atggcgggcc agatcacccc gccggcgaag
     3241 cccatgacca ccaacgcccc ggccgccgcc agctcggtgg tcgcgaaggc cagccagacg
     3301 tcggccacga tcgaggcgat ggtcgaggcg atgatgaccc gtctcgcgcc gagtcgatcg
     3361 atgacgtgac ccgtggggcc gaggacgatg aggctcacca cggcgggaat cgaggcgagc
     3421 acacccacgt gatcgagcgg gaacccgcgc acctcgtgca ggtagacgac gaggaacggc
     3481 atcaccaggc cggtgccgat gaagtcgatg atgatcgtgc tgagcagaaa cttgccctcg
     3541 cgcggcaggt cggtccagaa gtcgcgaagg cgcggccggg tcggcgtgcc gccactcccg
     3601 ccatcccgcg atgcgccact ccccccgtcg ctcgcctggt cgctcatgag gcacgagtct
     3661 gacaccgacc accgacagcc ctcgaccgga tttcggccgt ggcgggccgc tccggtaccc
     3721 tcgcccacgg aggattcgca tagcggccta gtgcgcacga ttggaaatcg tgttgggcta
     3781 aacaccctca ggggttcaaa tcccctatcc tccgccactg cggaccgccc gtcgccttag
     3841 gcgcgggcgg tcttcttttc ctggtatgcc gcgtagccgt ccgcctgccg ccgccacacc
     3901 gcggcgaggt gctcgcgcgg cataccggtg aggtccaggc cgaagacgcc ccgcagcacg
     3961 ccctcgaagt cggcggcgct ctccacaacc cgcttccgcc gcccagccgt gtcgatccgc
     4021 gtcaacgcca ccgactgcag caggtcgacg ccgtgggcgt cgcgccgctg gaccgcgagc
     4081 accttgacga acatcgactc cggcgaggtc gacaactccg tatgccgtgt ggtcgcctcc
     4141 gcgagcgtgc acggggcgat gtcgaggtcc attccgacaa acgactgggc gctcggatcg
     4201 tggtcaaacc gccacccgcc cggcacggcc tcggagggcc ggatgccgta agtgaacggc
     4261 ccctgtcgac aacgcccggg cgccaacggc agcggctcgt gcacagcgct gccgagaccg
     4321 acatcgacga gccacggctg cccttcgacg tcgacggtga gcgccaggtg attgccgtcg
     4381 gcccccaccg gggtcgcagc ccgcgccccc tggacgcctg cgcggtggtg ctgcacggca
     4441 tacccgaggc gctcgagcag ccaggcgaag gcgccgttga ggtggaagca gtagcccccg
     4501 tgcccgcgca ggaccgacgc gacggacgcg gcgacgtcga ctggcgtcgg gtgaccgagc
     4561 gcgatctgca gagtctcgta aggcacccgc tcgacgtggg cgcgatgcag cgcgacgagc
     4621 gtggcgcgat ccgcgggcgc ggccagcaga tcgcgtcgac cgaggcgagc caggtatgcc
     4681 gcgaccctcg ggtccgagcc gtccggctcg ctctgttccg gctcgtgatc cttcaccgcg
     4741 caattacatc aggccggtga gttgatctcg gttacgcgtt ccgtcgcggg ggcacagcgc
     4801 gcggatccgg gagttgacca acgtgccgca agatcgaggc cgccggtggc cctcggatcg
     4861 ccggatcggt ccctggagtc ggttacggct tgggcgccga agcggcctga cccccgacga
     4921 gcgcggtcgg cggtcgaggt tgcccgactg gcatcatgac cacatgacca cgcgggagcc
     4981 cgaagccatc gatgagggcg tcgtcgaact tgtcgagagc cttgagccca accccgccta
     5041 tgtcgcaggc gcacgctggg atgtgttgca tgccaaccgg gctgctcatc tgctcttcgc
     5101 cgacttcgcc agccggcccg accacgaacg caacatgctc tggtactacc ttgccgagcc
     5161 gagcgcccgg gccctctacg tcgattggga ggccgaggcg atcgcccagc tcggtcactt
     5221 ccgcgaggac tacgagaagt ggcccggcga cccgcgcttc gacgacctct tggagcgcgt
     5281 cttcgccgtc accccacagg ctcggacctg gtgggaggag cgccgcggcg tcgacctccc
     5341 ccgcaacggg gtcaagcaga tccgcctggc cgacgggcgc ctggtgagcc tgcggcagtt
     5401 ggtcatgagc accgtcgatg accctgagat ccaggtcgtg tgctatttcg ccgatatcga
     5461 cgacgacggt gattacggcg acgacatgga aagcctgctc ggagactgat cgcgccccct
     5521 ggcacgatag gcgcatgagc cctgggcgcg cggcataccc ctcgggcggc gactatccgc
     5581 ggttggcgcg gcgcctgggc ctgggagacg cggtcgtcat cgggctcggg tcgatgatcg
     5641 gcgcgggcgt gttcgccgcc ttcaccccag cggcagccgc agccggatcg ggcctgctcg
     5701 tcgggttggc ccttgccgca gcagtcgcct gggctaacgc gacctcgtcg gcgcaactgg
     5761 ctgcacagtt ccccgagtcg ggcggcacct atgtctatgg acgcgaatgc ctcggcgaga
     5821 gttggggatt cgctgccggc tgggccttcg tcgtcggcaa gacggcctcc tgcgcggcca
     5881 tggcgatcac cgccgcgacc tatgcgctcg ggggcgaggc gcgggcagcg caggtcgtcg
     5941 ggctggctgt cgtcggtgcc ctcaccgtcg tcctcacccg aggcatcacc aagaccgcgc
     6001 gcctggctcg ggtgctgctc accgtcaccc tcgcctgcct gctcgtcacg gtgctcgtca
     6061 tcgccgcctt cacccgcgag tgggccgcgc cctcggcggg cgtgggcgac gcgacggcat
     6121 acgggatctt gcagtctgcg gggctgctgt tcttcgcctt cgcggggtat gcccggatcg
     6181 ccaccctcgg tgaggaggtg cgcgcgccga cggtgaccat cccgcgtgcc atcacccgcg
     6241 cgttgggcct ggtcattgcc ctgtatgccg tcctcgccct cctgctcctg gccaccatcg
     6301 gcgcggacgg gatcgcggcg tcggcgctgc cgctgctgac ggcggcccag gcggccgacg
     6361 tgtcctgggc cggggtcttc gtgcgggtcg gcgcgaccgt ggccgccctc ggtgccctcc
     6421 tcgggctgct gtccggtgtt gggcgcacgg tgttggcgat ggcgcgccgc ggagacctgc
     6481 ctcggctgtt ggcggccgtc gatcctggct cgaaggtgcc cgctgtcgcg caactctggg
     6541 tggccggcgc cgtggctgtg ctcgtgctgc tcaccgatct gcggcacgcc atcgggttct
     6601 cgtcgttcgg ggtgttgctc tactacgcga tcgccaacgc gtcggcctgg actcagacgg
     6661 gcgagcaccg tcgctggccg cgctgggtcc aggcggccgg gctcgtcggg tgtctggcgc
     6721 tggccgcggc cctgccgatc ggttcggtgg tcgtcggcgc gctagtcctg ggtgtcgggg
     6781 tgttcggtcg ggtctggctg cagcgccggg gcgttgcccg cggctgagcg gcggttctcg
     6841 cagctgagcc ctgagcggtg agcgctgagt cctgagcgag tgtgtgacgg agtgcgattg
     6901 gcggggtgat caccccgtca atcgcactcc gtcaagcaca cgcccgcagc agcccgcccc
     6961 gagcaactct cagcggcggc gagacgtgcc aaagatcgac cgggtgatct cccgcccgat
     7021 gacggtgccc gccgagcgca gcatcgactt gaacaccgac gacccgacga cctgctcgac
     7081 caccgtcggc tcctcacgag ccggtcgagc agccgggcgg tcggccttgg cctctgcctt
     7141 ggcagccgcc tctgcggcct tggccgcctc tgcgtcggcc ttggcttgcg cttcggccgc
     7201 cgcggcctgg gcagcggcgg catcggcggc cgacgcggca gccgtgagct tctcgaaggc
     7261 cgactcacga tcctgcgcgg cggcataccg accggtgccg ggcgacgccg cgacgatctg
     7321 gtcgatgacc gcgtccgcgg agggagccat gagcgactgc ggcgcgcgca tccgggtcca
     7381 ggcgaccggc gtcggggcac ctcgctcgga cagcaccgtg atgacggcct cgccggtgcc
     7441 gagttgggtg agcaactcct ccaggtcgta ggcgctcttg gggtaggtcg acactgccgc
     7501 cttgagggcc ttggcgtcgt cgggggtgta ggcgcgcagc gcgtgctgga cccggttgcc
     7561 gagctgggcg agcacgtcgc cggggacgtc cttgggcgac tgagtgacga agaagacacc
     7621 gacgcccttg gagcggatga gccggaccgt ctgggtgatc gcctgcagga aatccttcga
     7681 cgcgttggcg aacagcagat gcgcctcgtc caagaagaag acgagcttgg gcttgtcgag
     7741 gtcgccgacc tcgggcaggt cgtggaagag gtcagccaac agccacatga ggaacgtcga
     7801 gaacaactgc ggccggtcct gcaccgcggg caactcgagc atcgacacga tgccgcgtcc
     7861 gtcggcaccg aggcgcagca ggtcggcggt gtcgaactcc ggcagcccga agaacacgtc
     7921 cgcgccctgg tcggcgaagg tgatcaactc acgcagaatg acgcccgccg tcgccgacga
     7981 cagcccgcca aggttcttga ggtcggcctt gccctcctcg ctcgtcaggt gctggacgac
     8041 ggcgcgcagg tccttgatgt cgtcgagcca caaacccttg gagtcggcgt agtggaacac
     8101 caggccgagg ctcgactcct gggtttcgtt gagccccagc acctttgcca gcagcgtcgg
     8161 cccgaaggaa gagaccgcgg cgcggatcgg gatgcccttg ccctggcctc ccagcgcata
     8221 gaactccgtc gggtatgccg tacccaccca ctcctcgccg atgtcggctg cgcgggcggc
     8281 caccttctcg ttggagacgc cgggcgacgc catgccggag aggtcaccct tgacgtcggc
     8341 gaggaacacg ggcacgccgc cttgggacaa ctgctcggcc atcaactgca gggttttggt
     8401 ctttccggtg cccgtcgcgc ccgccacgag gccgtgccgg ttgatgacgg ccagcggaat
     8461 cttgacttgg gcttcgggat aggcggcccc atccaggacg gccgcgccga aggcgagggc
     8521 accaccggcg aactcgtagc cggccttgat ctgctggatc tgctcgggca tctcggtcac
     8581 ggaacgcact ctagacgtac ccgcgcgcaa cgaacgcgag gtggcgcgcg gcataccgga
     8641 ggccgcaacc gggccaccac ctagactggc gcccgtgatc ttcaagggga taggcgagca
     8701 ccggccctac cccgaccatg ggctggtgac gacccgcgat tgggccaacg tcgcgccgcg
     8761 ccaggtgcgc ctgcaggacc tcgtgaccac caagcgcacc ctcgacttgg cgcagttgct
     8821 ggccgacgac tcgaccttct acggagactt gtttgcccat gtcgtcgagt ggcgcggcgt
     8881 cctctacctc gagcaggggc tccaccgggc gctgcgagcg gcccttcaac agcgtgggac
     8941 tctgcatgca cgcgtgctcg tgcttgacta gggcactgtc tgggctgtcg gggtcgcagc
     9001 acttcactgc ggaggtatgc cggtgagcat caccgtcgaa tcagcgtcgg tggcgcgagc
     9061 gcgccgtcgc aagcgaacag cgatcacgct gctcgtcgtc attgccatgc tcgccggtgc
     9121 gttctactac gcctcgagtt attggtcgaa gggctccacc aaagcggcgg ctccggcctg
     9181 cacgatcacc gcgggcccga gcggcccggt ctatccgagc caggtcacgg tcaacgtcta
     9241 caacgcgacc tcgcgcaccg ggttggccgc taccgtgggc cggttggtga aggcgaaggg
     9301 attcgtcctg ggcaccgtgt cgaacgaccc ggccaagaag cagatccccg gcaccgccga
     9361 ggtccgttac ggcagcgccg gtgagcgggc cgcggccctc gtgaagaccc tcgtcgccgg
     9421 tgccacgctg gtcaaggaca cccgagccga cgcatcggtc gatctggtgc tggggtcggg
     9481 ttacaaggag atggcgacct cgaccggcac ggcgaccctg gcgaccgcac cccccggctg
     9541 ctgaccttca gtcggcgtcg gcgaggggac gggtcatccc cccgagcaac accggcagca
     9601 tccgagccac gaggttgtct cgggagttcg catcgcccgc gatgatcgcc gagcgcagac
     9661 tcgtctcaaa cagggagtcg agggtgtcca acgcgtcatc cggagccacg gtgagcgcca
     9721 accccgcttg ggccagccct tccagcagga acggaacgaa ccgctcccgg caggcgcgct
     9781 gcgcagcgag gtaaggcgcg gcaagctcag ggtcgcggac cgcgagcaac gacaactcgg
     9841 cgcgcagtgc gtagttctgc gccccgaacg gctgcaggtc gaacaggcgc ccggcgattg
     9901 cggtcaacgg ggccggctcg gacagcgaag cacccagcgc ggtgctcagc cgctcgagga
     9961 gcagcccttc ctcgcggtct agaagggcca cgacgagctc gtccttcgtc gcgaagttgg
    10021 aatagaacgc tccgcgtgtg tagcccgccc gttcgcagac ctcctcgacg ctcgcccccg
    10081 ccacgcctcg ctcggcgaag acctcgccgg cagcatcgag caaccggtcg cgggtcacgc
    10141 tgcggctgcg tttggggtgg tcggtgtgct cagcgtggtc ggagtggacc atgagcccgc
    10201 tggcttccgc ggacaggccg ggctcaacac gggcgttcga cggggtggtc atctcgttct
    10261 tcaggctagt ggcggcatcg ggctgaacga cagcggatac accagtgtat ccaatacgtt
    10321 ggcgcatcca atacacttcc gtattgaacc gtcgtcgctc tcgggagtcc ggatgtcctc
    10381 agcgctctac gctctcggcc attgggccgt gcggcgccgc cgcctcgtcg tcgccctctg
    10441 ggtggccctc atcgtcatcc tcggcgccag cgcggggctg ctgcagcgcg ggatggccga
    10501 cgccttcgag atcccgggca cccagtccca gaaggccatg gacgccctct cctcgcgctt
    10561 ccccgaattc gccggggcca gtggccaagt cgtcgtctcg gccccagcgg gctccaaggt
    10621 caccgacccc tctgtcaccg cacgcatcga ggacctcacc gcaaggtatg ccgccctccc
    10681 gcacgtcagc ggcgcgacga gcccgttcaa cgaactcgtc aagggcaccg tgtcgccgga
    10741 cggttcggcc gcgatcatcg ccgtgcaact cgacctggcc tccgacgacg tcactgatgc
    10801 cacccgcgag ccgctcatcg ccctggccca cgaggccacc gcgacgggtc tgacgacgtc
    10861 ggtcggcggg gcgctgttca tgccgacagc tcccgagatg agcatcaccg agggcttggg
    10921 tgtcgtcgtg gcgctgatcg tcctggtggt cacgctcggg tcggcgatcg cggccggcat
    10981 accgctgctc acctccctgc tcggggtggc gatgtcgatg tcgttcatcc tcatcgcgac
    11041 ccacttcacg acgatcacct cgaccgcgcc gttcctcgcg ctgatgatcg gcctcgccgt
    11101 cggcatcgac tatgcgctgt tcatcctgtc gcggcaccga gacctgctcg ccgagggcgt
    11161 ggaggtcgag gaggcagcgg cccaggccac gggcacggcg gggtcggccg tcgtcttcgc
    11221 gggcgccacc gtcgccatcg cgctgctggc cctcggcgtc gcccagatcc cgttcctgtc
    11281 ggtcatgggc atcgctgcgg cggtcggtgt cgggtgtgcg gtcgtcgtcg ccctcaccct
    11341 gctccccgcg ctcatcggcg ccgcgggcac ccggctcacc cccaagcggg tcgccgacgc
    11401 gcaccatgcg cggcggcata ctagttggtc agcctcgtgg gtgcggctgg tgacgaaggt
    11461 gcccgcgctg accgcgatcc tcgtcatcgg cggtctcggc gtggccgcgc tgccggcccg
    11521 cgacctcacc ctcgccctgc ccaacaacgg gacgtcagcc cccgggtcga cccagcggga
    11581 ggcgttcgac acggtggcgc gcgccttcgg gccgggtgcc aacgcgccgc tcgtcatcac
    11641 cctcgacatc gtcgcgacga ccgacccact cggagtcgtc gccgacatcg agaagatggt
    11701 gctggccacg cccgggatcg cctcgacgag cgtcgccacc ccgaaccgcg gcgccgacac
    11761 tggcgtgatc gtcgcgattc ctgagggcag cgccgagagc caggtgacca aggacgcgct
    11821 gacggcggtc cgggcgctgg cccccgaggt ggagcgccag ttcggcgtcg agctcgcggt
    11881 gaccggccat acggctgccc aggtcgacgt ggccagccgg ctgtccgatg ctctgctgcc
    11941 gttcgggatc gtcgtcgtcg ggctctcgct cgtgctgctc atgctggtct tccggtccct
    12001 gctcgtgccg atcactgcgg ccgtgggcta cctgctctcg ctcggggcat ccttcggagc
    12061 ggtgagtgcc gtcgcccact ggggctggct ggccgcgccg ctcggagtga gccagaccgg
    12121 gccggtcatc gcgttcatgc cgatcatcgt catgggggtg ctgttcgggc tggcgatgga
    12181 ctaccaggtc ttcctcgtct cgagcatgcg ctcgcgctac gtccacggcg atgacgccaa
    12241 ggacgcggtg tcccatgggt tcgtcgatgc cgcccgggtc gtcactgccg cggcgatcat
    12301 catggttgcg gtgttcgcgg cgttcgtgcc caagggtgcg ctctacatca aaccgatcgc
    12361 tctcggcctg gccgtcggcg tgttcgtcga cgcgttcgtc gtgcggatga ccctggtccc
    12421 ggccgtcatg cggctgctcg ggccggcggc ctggtggctg ccccggtggc tcgaccggct
    12481 gttgccggtg ctcgacgtcg agggcgagag cctgcaccgg cggatcgctg ccgacgagac
    12541 tcggacggcg cgggcggcta tcgagggtcc ggtccccgtc atcgaggctc acgggctggg
    12601 ggtgtccgac ccggtcggga cggtcttcgc cgacgtcgac ctgagggtcc cggagggcgg
    12661 catcctcgtc gttcatggcg cgccgggagg cggcaagacc tccctgctgc tcaccctcgc
    12721 cggccggatg gccttcaccg caggctcgct cgacgttgcc gggcggctgc tgcccgacca
    12781 gcgtcacggg gtgcgcaagg ccacggccct cgccgagatc accggcgtca acgacctcga
    12841 ccccctcctc agcatcggcg accacctcac cgaacggctc gccggccgcg gctggctgcc
    12901 ctgggtcggg gcgcacgccc gtgaccgagc caccgggctg ctcaccgacc tcctcgggta
    12961 tgccgtgtcc gccgtcccag ccgcccacgc ctccccgtcg ctcgcctcca cgacgcgggt
    13021 gcgtgacctg gcccccctgg agcgctgggt cctgggcgtc accctggccc tgctcgacga
    13081 ccccgagatc ctcctcgtcg acgacatcga cggcctgcgc ggctccgacg accgggccgc
    13141 cgcctgggcc gtgctggcct cgctggccgg gtcacaggtc cgccacaccg ctcgcaccca
    13201 gccggctcgg gcgctcaccg tcgtcgtcag ttgccgcgac ctgcatgagg cgaccgacgc
    13261 gctggccggg gcgggcggca tacccctcgt gtcgttggcc ctcgcgagcc gcagcgccga
    13321 ggctgactcc ttcgtcaccg aacacaccgg cgacccgtgg accgaccccg tggccgccag
    13381 cttccccgac cccgccgacg agctctccga gaaggtgcac tgatgtcccg ccccgccctg
    13441 cctgccgccg agctgcgccg gttcgccgga agcccccggg ccctgctcgc gctcttcgcg
    13501 ctcgtcctcg tcccgctcgt ctatggcggg ctctacacgt gggccaacga gagcccgacg
    13561 acgcgggtcg accagatcgc cgcggccgtc gtcaacctcg acgagccggt gacgctcaag
    13621 ggcgccgacg gcaaggacca gatcgtgccg ctcgggcgcg tcgtggtcgg caagctgacg
    13681 tcgtcggcct cctcgtcgaa ctacgcgtgg accctcactg acgccgcgac ggccgaatcc
    13741 gggctcaagg agggcagatt cggcgcggtg ctgacgatcc cggccgactt ctcgcgctcg
    13801 gcgacgtcga ccaacggagc gcccgccgac gcgcgggccg cacggctgtc gttgcgcacc
    13861 gacgactcgg tcaactacct cgacggggcc atcgccaagg cgatcgcgac gacgaccagc
    13921 gccgaggccg cccgcaccgt cactgagggt tacctctcgg cgatgtatgc cggttacggc
    13981 accttgcacg accaactctc ctcggccgca gatggggccg gcaagctggc ctccggcgtc
    14041 accgagctga cgtcgggagc cgacaaggcc gccgttggcg ctgggtcgct ggcgtctggc
    14101 gtcgaccaga tcgcttctgg tgcaggggat ctcgcgtccg gggccgacaa actggcgacc
    14161 ggtgccgatc aggccgcggc cggagtggga cagctggcag gcggcctgtc ccagatcgac
    14221 tcgaaggttg cggcgatgcc cgccggcact cgggcgctgg ccgacggggc agcccaggtg
    14281 tcggccggac tcacggggct gtcgtccgga gcgggccaga ccgcttccgg tgcggcggca
    14341 ctcgcgactg gcctcacggc atacacctcg ggggttgacg ggctcgccgc cacgtgtgct
    14401 gcctcgggcg ctgcgcctgc cttctgcgcg aacctgtcgg gcctcgccgc acagggtggc
    14461 gggctgcgcg acggcgcgac cggggtgtcc ggtggcaccg ctcagttgtc cggtgggctc
    14521 agccaattgg ttcctggggc aacgcagttg gccggcggtg tcgaccaact tgccgacgga
    14581 atgcccgcat tggcggcggg tgtgcaccaa tcggccaccg gggcagcaac tttggcgagc
    14641 aagctccccc aactcgcgac gggcgtgcgc ggggtggccg acggagccga ccaactggcg
    14701 tcgggagcga cgaccgcggc taccggcgcc gatgagctga cgtccggttt gcaggcgctc
    14761 tccgacggca gcgccaagtt gggcaccggt gcgagcgatc tgagcaccgg gctcggcgaa
    14821 ggcgtgagca agatcccgac ctattcggag gccgagcggg cgcaactcgc cgcggttgct
    14881 gcgacgccgg tcgtggctga tgtcgctcgg gacaatgccg tcaagaacaa cggcgccggc
    14941 ctggctccct acttcatggc cttggccctg tgggtcggct cgctcgccat ctacctgctg
    15001 ctgcgtccgc tgtcgccgcg ggcgctcgcc tcgggcgccc cgtcgccgct ggtggcgctc
    15061 gccggatatc tgccgggcgc gctgctcggc gccgctcagg gcgctgcgct ggcgttggtc
    15121 ctgcagttct ggatcggcat cggtgccgcc tcgacgtggg ggctcatcgg ggtcgggatg
    15181 ctcatcgggc tgaccttcgt ggccatgaac caggccctcg tcggcctgct tggcgcaccg
    15241 ggtcggttcg tcgcggtgat ccttgccggg ctgcagttgg cgtcggcggg tggcacctat
    15301 ccggtcgcca ccgcaccggg cttcttcggc tggctgcacg acgtcctgcc gctcacctac
    15361 gcggtcgatg cgctccggcg ggtcgtcgcg ggagccaccg acggtctcgg caccgacatc
    15421 accgctctcg tcctcacctt ggtggggtcg ctcctggtga ccatgtatgc cgcccaccgc
    15481 cagcgcacct ggaccgtccg gcgcctgcgc cccgtcactg tcgcctgaga cctggcaggg
    15541 gactgatgag ttcccgggga acgtggcaac gtccggcgga tgaagtcgcc gatgaagtcg
    15601 cctccactgc tgtcccagcg acagcggtgg cgacttcatg ggcgacgtca tgaatcgctg
    15661 ctgcgccaac agccccaagt cgccaatcac acaccccgaa acgaccaaca gcccgaaccg
    15721 gcgagggttc gggc
//
LOCUS       DAOMZY010000029        15549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_3423_length_15549_cov_7.732477, whole genome shotgun sequence.
ACCESSION   DAOMZY010000029 DAOMZY010000000
VERSION     DAOMZY010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 15549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15549
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3423_length_15549_cov_7.732477"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="PLK64_03000"
     CDS             <1..586
                     /locus_tag="PLK64_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPV78738.1"
                     /translation="LGNHDYTDPGAGLAAYSAAFPHVPTDDDPQGRWYDQTIGDITVF
                     VLDSEAPPQHQVTQQRWLEDTLTEAAATAPDTWRLVVFHRPAHSSGHHGSHAPMQEAA
                     GWELQDWGADLVLNGHQHIYEDVLVDGLHHVTAPTGALGTVRECPAELVPQSRVCIEG
                     AGALRLTATSQTLHVELHLADAPDTAADRITLHR"
     gene            659..1246
                     /locus_tag="PLK64_03005"
     CDS             659..1246
                     /locus_tag="PLK64_03005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV78739.1"
                     /translation="MTEHAHDHAPGPLADASVRRAGPNDAAAVGLVQEAVWAETYAPR
                     VPEHIAAQFTSAGFASVWRRSLASPPPGVWTLLVACAREQVVGYAAVGPSQDRDGEAT
                     TGSLMEIGVHPKGRRSGHGSRLLNAAADILREAGATDLTAWLPADAEATRAFFDQANL
                     HPDGAYRDRGVAEDVTVREIRVAASLEPPSDPSRP"
     gene            1447..2565
                     /locus_tag="PLK64_03010"
     CDS             1447..2565
                     /locus_tag="PLK64_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78740.1"
                     /translation="MANGNDNSLGTQVIVDDTLNTDNSVNTETDVELEVEDSFNTDNS
                     TTTETDVELDVEVEDSFNDNSDNSVDNSDNSVDNSVENEADDSFNTDVEIEESFNTDN
                     SVENEAEDSFNDNSDNSTENEDSFNDNSDNSINTDIEVELEDSLNDNTDNSVRNQDSF
                     NITSSFNTQTLNDHSTTVGIREYQAGFGGDFNFGLGGGAMAAGGGWGKHGASGADMEV
                     DLDNRVMQLDQSVNQAISTEGGPGGGNVGQVFGQNATVSFGDGSNAAGRDVNIDNSEL
                     DLEVGDINIGNTDISTRINDSFNDYSTEMDMEWDLEIEDSFNDQSTNTEVEVEVDDSF
                     TSEVDAAFDNSLEWENSGNIFSPGAATTGGEAETEFDF"
     gene            2620..4557
                     /locus_tag="PLK64_03015"
     CDS             2620..4557
                     /locus_tag="PLK64_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HPV78741.1"
                     /translation="MFNSQGTTTDGEYMMGKTAEGTRSDSALTRRQRRELDGSGAAGP
                     AEDLLRLADDLAAMAQDTKRDDLVQRLGTAVERLRSPNTRVVVVGEFKQGKSKLINAL
                     VNAPVCPIDDDIATSVPTIVGFGPRPAASIVARSGDEIERHEIPIDELAQYVSESGNP
                     GNSRHLLAGEAQLPRAILQGGLQLVDTPGVGGTESQSALTTLSSLSTASAVIMVSDAS
                     QEFTEPEVQLLRHAMRVSPNVATVLAKTDLYSQWQDVQDIDRRHLREIGEVPLFAVSS
                     NLRLRAAQEQDRELNDESGFPALVAHLRREIVGRAELIAVRSAVHDLGFVVAQMELAA
                     RSELSALLNPEDSARLLAELDRAKARSEEFKSRSSKWNVRLGDGITDLIADMEHDLRD
                     RLRRVQREAEQAIDEGDPGPIWDQISQWLDERIASAVSETFIWTEERSHWLVEEVAEL
                     FLAEEVEIPLIAVGNTDGVLDQVQRLPGFEPGHLSAGEKIYIGVRGSYGGVLMAGLAT
                     GLIGLSLINPLSLLAGVLVGRRAYREDAANRLNRRRFEAKNIVRRHVDEVTFQVAKVL
                     RDRLRVVQRTGRDHFGAIADEMHRSLAEATLAAKQGAGARQEDDKKRIAWLRGHIQQL
                     ERVKAELPALPPATAKDVVVR"
     gene            4554..6197
                     /locus_tag="PLK64_03020"
     CDS             4554..6197
                     /locus_tag="PLK64_03020"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HPV78742.1"
                     /translation="MSVPVEVAVRDLLDTARALYGGDAPAQLDDLERRLAEPLRLAIA
                     GIVKAGKSTLLNAIVGERVAATDAGECTRLVTWYRHATTRSVSVVARDGTSTRLPIHR
                     AAAGHFAFDLNLPVDDVDRVEVGWPAPALKRVVLIDTPGIASLSPGTTARATGFLAPD
                     EAAPAADAIIYLLRRLHPADMRFLAAFRDTAAGSSRTVNAIAVLSRADEIGSGRIDSL
                     LSASKVARRYELDEHLRALALGVFPVAGLLAEGARTMRQNEFEGLRELAGLAREDREA
                     LLVSADRFVRLTGITTLGTDERRALMSRFGIFGVRLAASLIRAGVPDAPALTSEMTRQ
                     SGLDDLVEFVDGQFIGRTVALKAHGILEVLRVLLDQHPVDGASELEEGIERVSLRLSD
                     VDELTLLSRARIEGLGLGAREDEAIRIVGGHGVAVHDRLGMDAGAEPIRLRQRLVERL
                     DGWRAASHDPANDRRAVEAHEVVIRTLEELHEGLRARGEESRSVTSPAGGSELGPADT
                     DDRAAADVMLSRGPRDGAGEDAHEHGEQSQAHLRGHQLP"
     gene            complement(6706..7596)
                     /locus_tag="PLK64_03025"
     CDS             complement(6706..7596)
                     /locus_tag="PLK64_03025"
                     /inference="COORDINATES: protein motif:HMM:NF038175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IniB N-terminal domain-containing protein"
                     /protein_id="HPV78743.1"
                     /translation="MTTPMATIADALIEFILSLLGDPEAAAEFAEDPEGAFEAAGLGD
                     ICAADVRSVAPVVIDRPEVISVSPPDVTVNTPPTIVFRPPHPEEPKHELIREIANIVN
                     NFSFDNRATILDQSVNQSIWAEGDVTQLFDQDAVVAAGDESVAGGENVDIDNSTTDIE
                     AGDISIGNTDTDVEIEDSFNDESVDVELDLEADVEDSANDQSSTVEQDVSVEDSFNES
                     TETTIDGSTVVNAPASTPQVEESAAAAAEPVQEVADYAAETTPDSSLDTIDSISDSPE
                     YTDEALIEEQPLDAEFDDQP"
     gene            complement(7701..10238)
                     /locus_tag="PLK64_03030"
     CDS             complement(7701..10238)
                     /locus_tag="PLK64_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPV78744.1"
                     /translation="MTVQPLLTGAHLADAQIERILTSSRPTRAQVIGPAGSGKTAALE
                     FVRDGLDDRGVETHTFGAEVDIAAIPAEAVLIVDDLHLIDEEGAAPITERAHTPGAGL
                     VVALRPGPVGEHVRTIERLLQRSSPPIILGEVSATEVDAHLRAQGRPASCACIEDILD
                     LTGGAAWLVTFALSHDHVAECRGRTDHLALRRDLQNQVLQRVATLDAPLRRFIEQLSL
                     GTDPGRPPDGHAEALDELVLLGHAEGLLFRNGRMVPAVRTAMRSSVTHRWGSTDSTSL
                     AEEITRGCLEDDDSIELLLDGVRDPRVGDAIAVRADELRDDRPDHADRLYRVALRAGT
                     RSSDLAQRRARAAWAAGDLDRTGLLVDEAITKASSPADAGLADLAASVLAARDLMAMA
                     ARVYGSRTYEGATAVRAEIAHIGSGHLEPHRAPVVLPDDQGVSSQLDVALYLLHRGLR
                     SSLGRTPSPTALSDLVRASELYTASGTACPTPELPAVIAASVALGSGDLATARQVLDD
                     AVTGGQGRQWARRRLLLWQGLVAIQAERPDAARRAYTLAQQESAPVGARDARLGHILE
                     VTLARRFGDLAALQASWADVRGAVAHPDVDLYTIFPLATLVGAAARLGDSTTLAAHLQ
                     LGLDLLDRLGSPPLWSTHLWWAGVQQGILLNTPDALAPFAKALVGAADRSPLAATMAS
                     AGGVWVQVLAGKVDPDAVEDAARALGSAGLAWDGGRLAAHGARRSTDRRVSARLLSCA
                     RDLHPKESTSAAVTQPETAGVAAVGAESELSEREREVARLILKGKTYVEIGEAIFISP
                     RTVEHHVAHMRRRLEAGSRSDLIAKLRLALGATQDFARESPNRAVRV"
     gene            complement(10242..11558)
                     /locus_tag="PLK64_03035"
     CDS             complement(10242..11558)
                     /locus_tag="PLK64_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPV78745.1"
                     /translation="MTNSYHLGVDVGASRVHAATARYTSSRNERVEPCVLSTTGDDMS
                     ASMWVSREGDLEFGAIASAWAREQPEAIVGDLRRRIGTGIPFLVAGHRVFAEEAYAQL
                     VAWVVGAVTEIEGVPPVGVVLTHPVHWGAHRREGVRQAVEEHCRVPVDLTPEAEAAAW
                     GAVDEAQAGELWATCDLGHTSLATAIVRRHEDGWTVLPEAFGTAPLGGLDVDDALVGS
                     LLSDRATARSPVRAPSAELRSASVAAKEALSTDTDAVLALQFTPGPSTVRLTRDEFES
                     LIGELLDQTVCAVRQTLTDGGVTAEDLSGVVLAGGSARIPLVTERLSTALGVSALAEP
                     DPGAVVAMGAARMALRKSTGDLAAEPDSGSSRDEPLRPNDQVAALIPFIPPERARLAT
                     YAWRLVVASLIVVCAVYLAWAFTSDSVGVFGLGGHADSVVEPPRGG"
     gene            11875..12657
                     /locus_tag="PLK64_03040"
     CDS             11875..12657
                     /locus_tag="PLK64_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HPV78746.1"
                     /translation="MSEAAPTGELTPERRRAVRRQSWSVGLATGLYDVSFGALSVAAG
                     LTIWQTQALSAPMFTGGSQFALVGIIRTGGLAAAPAAIATATMLSIRNGLYALSTRRF
                     LLTGAAHTPVVRKAVVRTAAAAHLTIDESSAVGLAQPEPKAARLGFWHTGLAVFLFWN
                     LMTLVGALAGSVIGDPATYGLDGAAVAAFLALLWPRLQASDARVTAAVATVLALVAVP
                     FVPAGVPVLIAASAAVVIGLRGGRPGATAPPPDPATDGDVIP"
     gene            12654..12965
                     /locus_tag="PLK64_03045"
     CDS             12654..12965
                     /locus_tag="PLK64_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011773136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HPV78747.1"
                     /translation="MTLTTAVLIACALAFATKAIGYAIPPRLVEGERTMRVSTLLPAA
                     LLAGLIVVQTAADGPALVLDARVGALVVAALLLWRGAGFITVVIAGAVTAGLLRLIGI
                     G"
     gene            13141..14313
                     /locus_tag="PLK64_03050"
     CDS             13141..14313
                     /locus_tag="PLK64_03050"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HPV78748.1"
                     /translation="MNSPNSVARRVRRALVPLLGSTALVATMVAAPTATADEHWKVVA
                     SGLDNPRHLSVGPKGDLFVAEAGRGGGGPCIDSPEGGDSCFGRTGAITRVSLDKGKGK
                     KKGKQERVLSKLPSHAVEGGAQAIGPHDVIVEGSKKLVFTVGLGANPDVRTLPGNKPF
                     KDMGTLRSATIGSKRTTLIADIAKHEARKNPIHDPDSNPVGFIRHGSDYVVADAGGNT
                     LVEVSKRGRTKTLAVFEDQLAFAPPFLGLPPGTQIPSQAVPTSVVKGPGGALYVSQLT
                     GFPFEKGLAKVYKVDRQGAISVYASGLTNVTDLAFGPGNVLYAVQLVTEGLLTAETTG
                     PLGSVVAIPKGGGDSHAVVAANLFAPYGIAINGKTAYVTTGSVAPGAGQVVAIPLR"
     gene            complement(14399..15038)
                     /locus_tag="PLK64_03055"
                     /pseudo
     CDS             complement(14399..15038)
                     /locus_tag="PLK64_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136651.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
BASE COUNT         2482 a         5475 c         5300 g         2292 t
ORIGIN      
        1 cctcggcaac cacgactaca ccgaccccgg ggccggcctg gctgcgtact ccgccgcctt
       61 cccccacgtg ccgaccgacg acgaccccca aggccggtgg tacgaccaga cgatcgggga
      121 catcaccgtc ttcgtgctcg acagcgaggc cccaccgcag caccaggtca cccagcaacg
      181 gtggctcgag gacacgctca ccgaggccgc cgccaccgcg cccgacacgt ggcgcctcgt
      241 cgtcttccac cgccccgccc actcgtccgg ccaccacggc tcgcacgccc ccatgcagga
      301 ggcggccggc tgggagcttc aggactgggg cgccgacctg gtcctgaacg gtcaccagca
      361 catctatgag gacgtcctcg tcgacggcct gcaccacgtg accgccccga ccggggccct
      421 cggcaccgtc cgcgagtgcc cggccgagct cgtcccgcag agccgggtct gcatcgaggg
      481 cgccggagcg ctgcggctga ccgccacttc acagaccctc cacgtcgagc tccacctcgc
      541 cgacgccccc gacaccgccg ccgaccggat cacgctgcac cgctgagccg ggcaccacag
      601 ccgtccacac gccacgacgg atgtgtcccc gacccggcca ccgtgtgagg atcgacgcat
      661 gacggagcac gcccacgacc atgcacccgg cccgctcgcc gacgccagcg tccgccgcgc
      721 cggcccgaac gacgcggcgg ccgtcgggct ggtccaggag gcggtctggg ccgagaccta
      781 cgccccccgc gtccctgagc acatcgccgc ccagttcacc tcggccggct tcgcgagcgt
      841 ctggcgccgc tctctcgcca gccccccgcc gggcgtgtgg acgctgctcg tcgcgtgcgc
      901 ccgcgagcag gtcgtcgggt atgcggcggt cggcccctcc caggaccgcg acggagaggc
      961 gacgacgggt tccctgatgg agatcggcgt gcacccgaag gggcggcgca gcggccacgg
     1021 ctcccggctg ctcaacgccg ctgccgacat cctgcgcgag gccggggcga ccgacctcac
     1081 cgcgtggctg cccgccgacg ccgaggcgac ccgggcgttc ttcgaccagg ccaacctcca
     1141 cccggacggc gcctaccgcg accgcggcgt cgccgaggac gtcacggtgc gcgagatcag
     1201 ggtcgccgcc agcctggagc cgccctcgga tcccagccgg ccctgacggc atgaggggcg
     1261 ccggcgagga tcgacccctg acaatccccg tcgatccccg cccgaatccc tatccggtcg
     1321 ccgggattgt cagggacgct ccccgacgcg agctcggtca gagcgcggac agactgatct
     1381 caacgcgcca cccgggcgac gatcggaagt ccgaaagggc ctcccaacga gagaggaagg
     1441 acccttatgg cgaacggcaa cgacaacagc ctcggcaccc aggtcatcgt cgatgacacc
     1501 ttgaacaccg acaactcggt caacaccgag accgatgtcg agctcgaggt cgaggactcc
     1561 ttcaacaccg acaactcgac caccaccgag accgatgtcg agctcgacgt cgaggtcgag
     1621 gactccttca acgacaactc cgacaactcc gtcgacaact ccgacaactc cgtcgacaac
     1681 tcggtcgaga acgaggccga cgactccttc aacaccgacg tcgagatcga ggagtcgttc
     1741 aacaccgaca actccgtcga gaacgaggcc gaggactcct tcaacgacaa ctcggacaac
     1801 tcgaccgaga acgaggactc cttcaacgac aactcggaca actcgatcaa cacggacatc
     1861 gaggtcgagc tggaggactc gttgaacgac aacaccgaca actcggtgcg caaccaggac
     1921 tccttcaaca tcacgagctc gttcaacacc cagacgctga acgaccactc caccacggtc
     1981 ggcatccgtg agtaccaggc cggcttcgga ggcgacttca acttcggtct cggtggcggt
     2041 gccatggccg caggcggcgg ctggggcaag cacggcgcct ctggtgctga catggaggtc
     2101 gacctcgaca accgcgtcat gcagctggac cagtcggtga accaggccat cagcaccgaa
     2161 ggtggccccg gcggtggcaa cgtcggccag gtgttcggcc agaacgcgac ggtcagcttc
     2221 ggtgacggct ccaacgccgc cggacgcgac gtgaacatcg acaactcgga gctcgacctc
     2281 gaggtcgggg acatcaacat cggcaacacc gacatctcga cgaggatcaa cgactccttc
     2341 aacgactact ccaccgagat ggacatggag tgggacctcg agatcgagga ctccttcaac
     2401 gaccagtcca cgaacaccga ggtggaggtg gaggtcgacg actcgttcac cagtgaggtg
     2461 gatgcggcgt tcgacaactc gctcgagtgg gagaacagcg gcaacatctt cagcccgggc
     2521 gccgccacca ccggcggaga ggccgagacg gagttcgact tctgatcatc acgcgctgac
     2581 atccggtgcc cgatcgccct cgcggcgatc gggcactagg tgtttaactc gcaggggacg
     2641 acgaccgatg gggagtacat gatgggcaag acggccgaag gcacacgcag cgacagtgcc
     2701 ctcacccgtc ggcagcggcg tgagctcgac gggtccggcg ccgctggccc ggccgaggat
     2761 ctgctgagac tggccgacga cctggccgcg atggcacagg acaccaagcg cgacgacctc
     2821 gtccaacgcc tcgggaccgc cgtcgagcgg ctgcgcagtc cgaacacccg ggtggtcgtc
     2881 gtgggggagt tcaagcaggg caagagcaag ctcatcaacg cgttggtcaa cgcgccggtc
     2941 tgcccgatcg acgacgacat cgccacgagc gtgccgacca tcgtcggctt cggccctcgt
     3001 ccggccgcgt ccatcgtcgc tcgctcggga gacgagatcg agcgacacga gattcccatc
     3061 gatgagctgg cccagtacgt ctccgagtcg ggcaacccgg gcaactcgcg ccaccttctg
     3121 gcgggggagg cgcagctgcc ccgcgcgatt ctgcagggcg gactccagct ggtggacacc
     3181 cccggtgtcg ggggaacgga gtcccagagc gcgttgacga cgctgtcgag cctgtcgacc
     3241 gcgagcgcag tgatcatggt ctcggatgcc tcgcaggagt tcaccgagcc ggaggtgcag
     3301 ctgctgcgcc atgccatgcg cgtctcgccc aacgtggcca ccgtcctcgc caagaccgac
     3361 ctctactcgc agtggcaaga cgtgcaggac atcgaccgcc gccacctccg tgagatcggc
     3421 gaggtgccgc tcttcgccgt gtccagcaac ctgcgtctgc gggccgcgca ggagcaggat
     3481 cgcgagctca acgacgagtc cgggttcccg gccctggtgg cgcacctgcg cagggagatc
     3541 gtcgggaggg ccgagctgat agcggtgcga agtgccgtcc acgatctcgg cttcgtcgtc
     3601 gcccagatgg agctcgccgc acgaagtgag ctgagcgccc tcctcaaccc ggaggacagt
     3661 gcccgcctgc tcgcggagct ggatcgcgcc aaggcgcggt cggaggagtt caagagccgc
     3721 tcgtcgaagt ggaacgtccg cctcggcgac ggcatcaccg acctcatcgc cgatatggag
     3781 cacgacctgc gggatcgtct tcgaagagtg cagcgggaag cggagcaggc catcgacgag
     3841 ggagacccgg gacccatctg ggaccagatc tctcagtggc tggacgagcg gatcgccagc
     3901 gcggtctcgg agacgttcat ctggaccgag gaacggtccc actggctcgt cgaggaggtc
     3961 gccgaactgt tcctcgccga ggaggtcgag attccgctga tcgccgtcgg gaacaccgac
     4021 ggtgtcctcg accaggtgca gcggcttccc gggtttgagc cggggcacct ctccgccgga
     4081 gagaagatct acatcggcgt ccgcggctcg tacggcggcg tgctgatggc gggcctggcc
     4141 accggtctga tcggcctgtc cctgatcaat cccctgtcgc tcctcgccgg cgtgctcgtc
     4201 ggtcggcggg cctaccgcga ggacgccgcc aaccgactca accgtcggcg cttcgaggcc
     4261 aagaacatcg tgcgccggca cgtcgacgag gtgaccttcc aggtcgccaa ggtgctgcgc
     4321 gatcgtctca gggtcgtcca gcggaccgga cgtgaccatt tcggggccat cgcggacgag
     4381 atgcaccgct ccctcgcgga ggccacgctc gcggccaaac agggggccgg tgcgcggcag
     4441 gaggacgaca agaagcgcat tgcgtggtta cgcggccaca tccagcagct cgaacgagtc
     4501 aaggccgagc ttcccgccct cccgccggcg accgccaagg atgtcgtcgt ccgatgagtg
     4561 tgcccgtcga ggtcgccgtg cgcgacctcc tggacaccgc ccgcgcgctc tacgggggcg
     4621 atgctcctgc gcagctggac gatctggagc gtcgtctcgc agagccgctg cggttggcga
     4681 tcgcgggcat cgtcaaggcg ggcaagtcca cgctgctgaa cgcgatcgtg ggtgagcgtg
     4741 tcgcggcaac ggacgccggc gaatgcactc gcctcgtcac gtggtaccgg catgccacca
     4801 cccggtcggt cagcgtcgtc gcgcgagacg gcacctccac caggctcccg atccaccggg
     4861 ccgcagcggg gcacttcgcc ttcgatctga acctcccggt cgacgacgtc gatcgggtcg
     4921 aggtcggctg gccggccccc gcgttgaaga gggtggtgct gatcgacacc ccggggatcg
     4981 cctcgctctc gccggggacg accgcacgcg ccaccgggtt cctcgccccg gacgaggctg
     5041 cgccggctgc cgacgccatc atctacctcc ttcgtcggct gcacccggcg gacatgcggt
     5101 tcctcgcggc ctttcgggac acggccgcag gatcctcgag gacggtcaat gccatcgccg
     5161 tgctctcgcg agcggacgag atcggctcgg gacggatcga ctccctcctg tcggcgagca
     5221 aggtcgcccg ccgctacgag ctggacgagc acctgcgagc gctggccctc ggcgtcttcc
     5281 cggtggcggg gctgctcgcc gagggtgctc gaaccatgcg gcagaacgag ttcgaggggc
     5341 tgcgcgagct cgccggcctg gcccgggagg accgggaggc cctgctcgtc tcggccgacc
     5401 gcttcgtccg gctgacgggt atcacgaccc tgggaacgga cgagcgccga gccctgatgt
     5461 cccggttcgg catcttcggg gtccgccttg ccgcatcgct gatcagggcg ggagtgccgg
     5521 acgcgcccgc cctgacgtcg gagatgacac gtcagagcgg cctggacgat ctcgtcgagt
     5581 tcgtcgacgg ccagttcatc ggccgaacgg tcgcgctcaa ggcccacggc atcctggaag
     5641 tcctcagagt cctgctcgac cagcacccgg tggacggcgc ctccgagctg gaggagggga
     5701 tcgagcgagt ctccctacga ctgagcgacg tggacgagct gaccctgctg tcccgggccc
     5761 ggatcgaggg actcggtctc ggtgctcgtg aggatgaggc gatcaggatc gtcggcggcc
     5821 acggagtggc ggtgcacgac cgtctcggca tggacgccgg cgccgagccc atccggctgc
     5881 gtcagcgcct ggtcgagcga ctcgacggat ggcgggccgc ttcacacgac ccggcgaacg
     5941 accggcgagc ggtcgaggcc catgaggtgg tgatcagaac cctcgaggag ctgcacgagg
     6001 gcttacgtgc gcgaggggag gaatctcgct ccgtgacgag cccggctggc ggctcagagc
     6061 tcggtcccgc tgacactgac gatcgtgccg cggcggatgt catgctgtcg cgcggtccac
     6121 gcgatggcgc cggggaagac gctcatgagc atggcgagca gagccaggcc cacctgcgcg
     6181 gtcatcaact gccgtagcga ggctccctgg tcgccgcgcg ccaccatctc ggcgatgacg
     6241 aggaacaaca ggaactggac cgccacggtt ccgaactgcc accacctggt gctcttgcgg
     6301 cggttcatga acgacaccag gagggtgatc tggacgagaa gcagcgtcac cataatcccg
     6361 aacgccgacc agtagacgat ctcagcggcg gaggtgtagg tctcctcggc ccgcgtggcg
     6421 ttgacctccc tgaccgcgtc ggcgatctgc gggagctgct cttcccgaat ggcgacgagg
     6481 aagccgacgg ccaccgtgcc cgcgaggaag ctcagcaccc acaggaactg cgccaggcgc
     6541 accgagaacg gcggcggctg cttgatcacg accgggggct ggttggggct ctccaacgac
     6601 ggacgcgcag gacgttccga gggctgctcc acggctggct cttccttcga tgtctgtcgc
     6661 gcgacgatcc catgggtcgc cggatccggg gatccgggct cgatgctacg gctggtcgtc
     6721 gaactctgca tcgagcggct gctcctcgat cagcgcctcg tcggtgtact ccggactgtc
     6781 cgagatcgag tcgatcgtgt ccagcgaact gtctggtgtc gtctcggcgg cgtagtcggc
     6841 gacctcctgc acgggctctg cggcggcggc agccgattcc tcgacctgcg gcgtcgaagc
     6901 aggagcattc acgaccgtcg acccgtcgat ggtcgtctcg gtcgactcgt tgaaggagtc
     6961 ctccacgctc acgtcctgct ccaccgtgct cgactggtcg tttgcggagt cctcgacgtc
     7021 ggcctcgaga tcgagctcga catcgaccga ctcgtcgttg aaggagtcct cgatctccac
     7081 gtcggtgtcc gtgttgccga tcgagatgtc accagcctcg atgtccgtcg tcgagttgtc
     7141 gatgtcgacg ttctccccgc ccgcgacgga ctcgtctccc gcggccacta cggcgtcctg
     7201 gtcgaagagc tgggtgacgt caccctcggc ccagatcgac tggttgaccg actggtcgag
     7261 gatggtggcc ctgttgtcga aggagaagtt gttcacgatg ttcgcgatct cccggatcag
     7321 ctcgtgcttg ggctcctccg ggtgcggggg gcggaagacg atcgtcggcg gggtgttgac
     7381 cgtaacatcc ggcggagaga cggagatgac ctccggccgg tcgatcacca ctggcgcgac
     7441 actgcggacg tcggccgcgc agatgtcgcc cagccccgcc gcctcgaaag caccctcggg
     7501 gtcctcggcg aactcagcag ccgcttccgg atcccccagg agactcagga tgaactcgat
     7561 cagtgcatcg gcgatcgtcg ccatcggcgt cgtcatatcc gtctctcctg ctcaggcgcg
     7621 tgtgggccat cgtgtctgtg atcgtatggg aatggtttca gtctaggagc cgcgttcggt
     7681 gcgtacatcg gggttttcac ctatacccgc accgctcggt taggggattc gcgcgcgaaa
     7741 tcctgggtcg cgccgagcgc gagtcgcagc ttcgcgatga ggtcggaccg cgagccggcc
     7801 tcgagtcggc ggcgcatatg cgccacatgg tgctcgacgg tgcgcggaga gatgaagatc
     7861 gcctcaccga tctcgacgta cgtcttgccc ttgaggatga gtcgggcgac ctcacgctca
     7921 cgctcgctga gctcggactc cgcgccgacg gcggcgacac cggcggtctc cggctgggtc
     7981 accgcggctg aggtgctctc cttggggtgg aggtcgcggg cgcaggacag cagcctggct
     8041 gagacccgcc gatccgtgga ccggcgcgcg ccgtgtgcgg cgaggcggcc gccgtcccac
     8101 gccagtcccg cggagccgag agcgcgggct gcgtcctcga ccgcgtcggg gtcgaccttg
     8161 ccggcgagga cctggaccca cacgccaccc gcgctggcca tcgtggcggc cagcgggctc
     8221 cggtcggcgg cgccgacgag cgccttggcg aacggggcca gcgcatccgg ggtgttgagc
     8281 aggatcccct gctggacgcc ggcccaccag aggtgagtcg accacagcgg cggtgacccg
     8341 aggcggtcca gcaggtcgag gccgagctgc agatgggctg cgagcgtggt cgagtccccg
     8401 agacgtgctg ctgcccccac caacgtcgcg agcggaaaga tcgtgtagag gtccacgtcc
     8461 gggtgcgcca cagccccacg gacgtcggcc caggacgcct gcagcgccgc cagatccccg
     8521 aagcgtcgag cgagggtgac ctcgaggatg tgcccgagcc gggcgtcccg ggcgccgacc
     8581 ggcgcggact cctgctgcgc cagggtgtat gcccgccgcg cggcgtccgg ccgttccgcc
     8641 tggatcgcca ccaggccctg ccagagcaac agcctgcgac gcgcccactg ccggccctgc
     8701 ccgcccgtga ccgcgtcgtc gaggacctgg cgagccgtgg ccaggtcacc cgagcccagc
     8761 gcgaccgacg cggcaatcac ggcggggagc tccggtgtgg gacaggccgt ccccgatgcg
     8821 gtgtagagct ccgaggcccg caccagatcg ctgagcgcgg tcggcgaggg ggtgcgaccg
     8881 agggacgagc gcaggccccg gtggaggaga tacagggcga cgtcgagctg cgagctcaca
     8941 ccctggtcgt ccggcagcac gacgggagcg cggtggggct cgagatggcc gctgccgatg
     9001 tgtgcgatct ccgctcggac ggccgtcgcg ccctcgtacg tgcgtgatcc gtagacacgg
     9061 gcagccatcg ccatcaggtc tcgggccgcc agcacggacg cggccaggtc ggccagcccg
     9121 gcgtccgcag gggagctggc cttcgtgatc gcctcatcca cgaggaggcc cgtccggtcg
     9181 aggtcaccgg ccgcccaggc cgcgcgagcc cggcgctgcg ccagatccga ggacctcgtc
     9241 cccgccctga gcgcgacgcg gtacagccgg tccgcatggt cgggtctgtc gtcccggagc
     9301 tcgtcagcac gaacggcgat ggcatcgccc acacgagggt cccgcacacc gtcgagcagg
     9361 agctcgatgg agtcgtcgtc ctccagacag cctctggtga tctcttcggc cagacttgtg
     9421 gagtccgtgc tgccccaccg gtgcgtgacc gacgaccgca ttgccgtgcg cacggcgggc
     9481 accatgcggc cgttgcggaa gagcagaccc tcggcatggc ccaggagcac cagctcgtcc
     9541 agggcctctg cgtgcccgtc cggggggcgg ccggggtcgg tgcccagact gagctgctcg
     9601 atgaaccgcc gaagcggcgc gtcgagtgtc gcgacacgct gcagcacctg gttctgcagg
     9661 tcccgccgga gcgcgagatg gtctgtcctg ccccggcact ctgcgacatg gtcgtgggag
     9721 agggcgaagg tgaccagcca tgccgccccg cccgtcaggt cgaggatgtc ctcgatacat
     9781 gcgcacgacg cgggccgacc ttgcgcacga aggtgtgcgt cgacctctgt ggcagagacc
     9841 tcaccgagga tgatcggagg agagcttcgc tggagcaggc gttcgatggt cctcacgtgt
     9901 tcacccaccg gcccggggcg cagtgcgacg acgagaccgg ctcccggggt atgcgcccgc
     9961 tcggtgatcg gcgcggcgcc ctcctcgtcg atgagatgga ggtcgtcgac gatgagaacc
    10021 gcctcggccg ggatggcggc gatgtcaacc tcggcaccga aggtgtgcgt ctcgaccccc
    10081 cggtcgtcga gcccgtcacg gacgaactcc agcgccgcgg tcttccccga cccggcgggg
    10141 ccgatgacct gcgctcgggt gggtctcgac gacgtgagga tccgctcgat ctgagcgtcc
    10201 gccaggtggg ctcccgtgag gagtggttgg actgtcatgt ctcaccctcc ccgcggtggt
    10261 tcgacgacgg aatcagcgtg cccaccgagt ccgaacacgc cgaccgagtc cgacgtgaac
    10321 gcccacgcga ggtacacggc gcagacgacg atgagagagg cgacgacgag ccgccacgcg
    10381 tacgtggcga gtcgtgcccg ttcgggaggg atgaagggga tcagggccgc tacctgatca
    10441 ttcgggcgca gcggttcgtc gcgggacgat cccgagtccg gctctgctgc caggtcgccc
    10501 gtgctcttcc gcagcgccat gcgagcggcg cccatcgcga cgacggcgcc cgggtccggt
    10561 tcggccaggg cggagacgcc aagcgctgtg gacagccgct cggtcaccag cgggatccgt
    10621 gcggatccgc ccgccaggac gactcccgag aggtcttcgg cagtcacgcc gccgtcggtc
    10681 aacgtctgcc ggaccgcgca gactgtctgg tccagcagtt cgccgatcag ggactcgaac
    10741 tcgtcacggg tgagtcggac cgtcgacggg ccgggcgtga actgcagggc caggacggca
    10801 tcggtgtccg tcgagagcgc ctccttggcc gcgacggacg ccgacctgag ctctgcggat
    10861 ggggcgcgca cagggcttcg tgccgtcgcg cggtcggaca ggagactgcc gacgagggcg
    10921 tcatcgacgt cgagcccccc gagcggagcg gtgccgaacg cctcgggcag aaccgtccag
    10981 ccgtcctcat gcctgcggac gatggccgtg gcgaggctcg tgtggccgag gtcgcacgtc
    11041 gcccacagct cccccgcctg cgcctcgtcg acagctcccc acgccgccgc ctccgcctcg
    11101 ggagtcaggt cgacggggac acggcagtgc tcctcgacag cctgacggac accctcgcga
    11161 cggtgcgctc cccagtgcac cgggtgggtc agcacgacgc cgacgggagg cacgccctcg
    11221 atctccgtca ctgcaccgac gacccaggcg acgagctgcg cgtaggcctc ctcggcgaag
    11281 acccggtggc cggcgacgag gaacgggatc ccggtgccga tgcggcgccg cagatcgccg
    11341 acgatcgcct ccggctgctc ccgcgcccat gccgatgcga tggccccgaa ctcgagatcc
    11401 ccctcgcggg acacccacat cgacgccgac atgtcgtcgc ccgtggtcga gagcacgcag
    11461 ggctccacgc gttcgttgcg cgaggacgta tatcgtgcgg tggcggcatg gacgcggctg
    11521 gcgccgacat ccacgccgag gtgatagctg ttggtcaccg gactccctga agtcactctg
    11581 actggtggac ggacgtccgg cgccgggtaa ggcaccactc gtccccccgt ccttggttcc
    11641 tcacgctacc ggcgattcag cgacgaggac gggaatcaag gatctactcg tcagtagccg
    11701 gagatccatg gccgttcggt cgatgagagg gtgtcgctct gggggccgcg aggtaaacgt
    11761 atgggaaccg gcggccctcc cctcggacgc gtcgaggtgc cattggtgtc aggccacgac
    11821 gggcgtcata cactgagtgc cgtttggtga cgacgatcgg cggccggagg ggcgatgagt
    11881 gaggccgcac cgaccggcga gctgaccccc gagcgccgcc gggccgtccg gcgccagtcg
    11941 tggtcggtcg ggctcgcgac ggggctgtac gacgtcagct tcggcgccct ctccgtcgcc
    12001 gcgggcctga ccatctggca gacgcaggcg ctctcggcgc cgatgttcac cggcggcagc
    12061 cagttcgcgc tggtcgggat catccgcacc ggcggcctcg cggcggcacc ggcggcgatc
    12121 gccaccgcga cgatgctgag catccgaaac ggtctgtatg cgctgagcac ccgccgcttc
    12181 ctgctcaccg gggccgcgca tacacctgtc gttcgaaagg cagtcgtgcg gacggcggcc
    12241 gccgcccacc tgacgatcga cgagtccagc gccgtcggcc tggcccagcc ggagccgaag
    12301 gcagcgcggc tcggcttctg gcacaccggt ctcgcggtgt tcctgttctg gaacctcatg
    12361 acgctcgtcg gtgcgctggc gggctcggtg atcggcgacc cggcgaccta cgggctggac
    12421 ggggcggcgg tcgcggcgtt cctcgccctg ctgtggccga ggttgcaggc gagtgacgcc
    12481 cgggtcacgg ccgcggtcgc gacggtgctg gccctcgtcg cggtgccgtt cgtcccggcc
    12541 ggcgtcccgg tgctcatcgc ggcatcggcc gccgtcgtca tcggcctgcg cggcggtcgg
    12601 ccgggcgcga ccgctccccc accggacccg gcgacggacg gggacgtcat cccatgacgc
    12661 tgaccaccgc tgtcctgatc gcctgcgcgc tcgccttcgc aaccaaggcg atcggctatg
    12721 ccatcccgcc gcggctcgtc gagggcgagc ggacgatgcg ggtctcgacg ctgctcccgg
    12781 cagccctcct cgccggcctg atcgtggtgc agacggccgc cgacggcccg gcgctcgtcc
    12841 tcgacgcccg ggtcggcgcg ctcgtcgtcg cggcgctgct gctgtggcgg ggagccggct
    12901 tcatcaccgt ggtcatcgcc ggagccgtca cagcagggct cctgcgtctc atcggtatcg
    12961 gctgaaccat cggtcacagc gcgtacaagg acagtgcagg ctctgcgtgg aaacccgcag
    13021 gtcagagcgc gattcctgac cgttgtgacc cttgtcacgg cgtcccggca gtgggactct
    13081 gtgagacggc acccatccgg gcgaccactc cttgtcaatg aagagaaggg ggactcccct
    13141 gtgaacagtc ccaactcggt ggctcggcgg gtgcgccgag ccctcgtgcc gctgctcggc
    13201 tcgaccgccc tcgtcgcgac catggtcgcg gcgcccacgg cgacggccga cgagcactgg
    13261 aaggtcgtcg cgagcggcct cgacaacccg cgtcacctgt ccgtcgggcc caagggcgac
    13321 ctcttcgtcg ccgaggccgg ccgcggcggc ggcggcccgt gcatcgacag cccggaaggc
    13381 ggcgactcgt gtttcggcag gaccggcgcc atcacgcggg tcagcctcga caagggcaag
    13441 ggcaagaaga agggcaagca ggagcgtgtc ctgtccaagc tcccgtcgca cgcggtcgag
    13501 ggcggcgcgc aggcgatcgg cccgcacgac gtcatcgtcg agggctcgaa gaagctcgtc
    13561 ttcacggtcg gcctcggcgc caaccccgac gtccggacgc tcccggggaa caagccgttc
    13621 aaggacatgg ggacgctgcg ctcggccacc atcggctcca agcgcaccac cctcatcgcc
    13681 gacatcgcca agcacgaggc gcgcaagaac ccgatccacg acccggacag caaccccgtc
    13741 ggcttcatcc ggcacgggtc tgactatgtg gtcgcggacg ccggcggcaa caccctggtc
    13801 gaggtgtcga agaggggccg cacgaagacg ctcgccgtgt tcgaggacca gcttgcgttt
    13861 gcgccaccct tcctcgggct cccgcctggg acgcagatcc cgtcccaggc cgtgccgacc
    13921 tcggtcgtca agggcccggg cggcgcgctg tacgtcagcc agctcaccgg cttccccttc
    13981 gagaagggtc tggcgaaggt ctacaaggtc gaccgccagg gcgccatcag cgtctacgcc
    14041 agcggtctga ccaacgtcac cgacctggcc ttcggcccgg gcaacgtgct ctacgccgtg
    14101 cagctcgtga ccgagggtct gctgactgcg gagacgacgg gaccgttggg gtcggtcgtc
    14161 gcgattccga agggcggcgg cgactcccac gccgtcgtcg ccgcgaacct gttcgcgccg
    14221 tacggcatcg cgatcaacgg caagaccgcc tacgtcacca cgggcagcgt cgcccccggc
    14281 gccggtcagg tggtcgccat cccgcttcgc tgagcagcca ggcggcgcag cccaggcccg
    14341 ggtttgcgct ggtccagtga acacagacgt ggccggtgcc gttgcaggac ggcaccggtc
    14401 acggcaacgt cggtcgctcc agctcgagca accgcgagag gtgctcgccg tagccggact
    14461 tcgcataccg ctcggccgtc tccgccagct gcgcgtcgga caggaacccc atccgccagg
    14521 ccgcctcctc gggcgaaccg atctgcagtc cctgccgcgc ctggatcgtg cgcacatagt
    14581 tgctcgcgtc gttgagcgag tcgaaggtcc cggtgtccag ccacgccgtc ccgcgcggga
    14641 gcttctccac ccacagccgc ccgcggtcca gataatgccg gttgacgtcg gtgatctcgt
    14701 actcgcctcg cgccgacggg gcgagcccac gggcgatctc gacgacctct tcgtcgtaga
    14761 agtacagacc cggcacggcc cagtgcgacc tcggctgctc gggcttctcc tcgatcgaca
    14821 gcgctcgccc gtcgtcggcc acctcgacga cgccataggg cccgcgggtc ggcgacctgg
    14881 tacccgaaga tcagcgcccc gtcgacggac tcggccctct cgcgcagccg gtagcccagt
    14941 ccgggcccgg agaagacgtt gtcccccagg accagacacg acggtccgcc ttcgacgaac
    15001 tccgcgccga tcgtgaacgc ctgggcgagc ccaccgggac caattcacca gggtgatgac
    15061 gctggcagcc atgtcaggcg cggcacgacg tcaggtcgct cccgaggctg agctcgaagc
    15121 ggcacggatg tcgcggattc acttccccac gggtggccac cgattccgtc cgaccctgga
    15181 agacgtcctt cagatgatcg aggaggagtt cggcgcgaaa cccggcccaa catggcgaga
    15241 ggtccttcgg acgaacaggg tgcagtggcg acgccagcaa accgggtctg cagtgcggga
    15301 ctgcccgagc gaggcggttc gggtcttgga gcagcttggg tacgccatct cggagccctt
    15361 cgacgggccc gcacccgatc gggaagatcg cctagcagcc ttctaggcga gtcgaggttg
    15421 cgtctcgatg cctggttgct tccgccagaa gctataacac tagctggtgg acacgcccga
    15481 ggtgcataat gtcggctaat tcatggaatc agcggcactt cttacagttt tgagtgcatc
    15541 tcgacagcc
//
LOCUS       DAOMZY010000030        15540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_3424_length_15540_cov_6.462448, whole genome shotgun sequence.
ACCESSION   DAOMZY010000030 DAOMZY010000000
VERSION     DAOMZY010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 15540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15540
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3424_length_15540_cov_6.462448"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PLK64_03060"
     CDS             <1..332
                     /locus_tag="PLK64_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HPV78749.1"
                     /translation="VVPATFGWDDIGDFDSLAGLLASHDSDASDDGDASVGGLSIVGE
                     SGDVLALDSTGLVVARSGRLVALIGVEDLVVVDTPDALLIVPRARAQEVKGIVDRLAD
                     LGRDHLR"
     gene            complement(354..1955)
                     /locus_tag="PLK64_03065"
     CDS             complement(354..1955)
                     /locus_tag="PLK64_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide antibiotic transporter"
                     /protein_id="HPV78750.1"
                     /translation="MTASLVGTGTLARLAVRRDRVLLPVGILGITIFAVSSAKATLAL
                     YPTVASMDAGIKTIFASPSAVALYGPISDPTDPDALAVVKTTMFGAVLLAIFGYAVVR
                     RHTRSEEEDGRFELVGAGVVGRRAPLTAAVAVATAAVLVTCVLTGLGYAAIGMDVAGS
                     LAAAAGWAATGLAFVGLAAVAAQLASTARGCAGLTMGALGVLFLLRAIADAGTGVPGW
                     LGWVSPVGWTSKAEAFGADRSWVVFLGVGLLAVCLVAAFALLQRRDLGAGLLPARRGR
                     ANAGATLAGPVGLAWRLGRSSTIGWTVGMLVGGLVIGSLAKSAMDMFADPAIADLLRK
                     MGGGVGLLSDVYLSTELGFIAIIAAAYGVTTVLRWRAEETQGHTELVLATADSRTRYA
                     ASHLLVALLGTAALVFALGLGTVLADASGGGTLGGAGRVLPAALIRLPAVWVCVGVAV
                     AAIGWFPRWTAAIGWGALAFFLVVGEFGGVFGMPQWLMDLAPFTHVPRMPVEAFAWPP
                     TIALTAVAAGLCIVGVLGFRRRDIG"
     gene            complement(1952..2839)
                     /locus_tag="PLK64_03070"
     CDS             complement(1952..2839)
                     /locus_tag="PLK64_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV78751.1"
                     /translation="MSAPIEISGLRKSFGTTVALDGLDLTVAQGEIHGFLGPNGAGKS
                     TTIRVLLGLLRADGGQARLLGGDPWTDVAQLHRRIAYVPGDVALWPNLTGGEVIDLLA
                     RLRGGLNAARRDDLLARFDLDPTKKCRAYSKGNRQKVALVAALSSDAELLLFDEPTAG
                     LDPLMDRVFRDHVDEVRTEGRTVLLSSHILSEVEALCDRVSIIRAGRTVESGSLAQLR
                     HLTRTSVTATLEHPPSAAELAALTGVHDVVLDGSRVTLSADTDHIGAVLSALGAHGIQ
                     TLTSQPPTLEELFMRSYAS"
     gene            2977..4560
                     /locus_tag="PLK64_03075"
     CDS             2977..4560
                     /locus_tag="PLK64_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPV78752.1"
                     /translation="MADARSESDLPIKPMYAATDLAAFDAATQLGDAGKYPFTRGVYP
                     SMYTGRPWTMRQYAGFGTAKESNERYHQLVAAGTGGLSVAFDLPTQMGYDSDESISSG
                     EVGKVGVAIDSIDDMRVLFDGLPLDKISTSMTINAPGSTLLLLYQLVAEEQGISGDQL
                     TGTIQNDVLKEYIARGTYIYPPKESLRLIADIFAYCQAEMPRWNTISISGYHMAEAGA
                     TPAQEIAFTLANAKEYVRAAQKAGLGVDDFAPRLSFFFVARTTLLEEIAKFRAARRIW
                     AKIMRDEFGAKNPKSQMLRFHTQTAGVQLTAQQPEVNLIRVALQGLGAVLGGTQSLHT
                     NSYDEAIALPTQKAARLALRTQQVIAYETDVCKTVDPFAGSYAIEAMTDELEVAIEDL
                     IQQVEDRGGAVRAIEQGFQKGEIESSAYRVALEIDSGERTVIGVNKFTVDVEEEYEPL
                     RVNPAIESEQRDRLAKLRAERDNDAVTAALEALKEAARGTENILYPMREALRARATGG
                     EVAHALREVWGTYIPTDSF"
     gene            complement(4592..5500)
                     /locus_tag="PLK64_03080"
     CDS             complement(4592..5500)
                     /locus_tag="PLK64_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPV78753.1"
                     /translation="MSTAAGARPIVVIGDVGMDVVAVPSGPIRYGTDTPAQVSMRRAG
                     AGANTAAWLGTAGADVTLVARVGDDSAGRTARAELERAGVRCVWAVDRDLPTGLVVVL
                     VDASGERTMLPSRGANAALRSTDVTGALDVVAAFPGGMPHLHLSGYVLLDPGSRAAGL
                     AALGEARRRGWSTSVDPQAAALVSEVGAAVFLDWVRGVDLLLPNANEADALGGVPTIL
                     ANVHAVAVTDGAAGARWFGAGGEVWEVAAEPVAAPDTTGAGDAFDAGLLAAWVAGADP
                     AAALAAGVCAGTAVITGRARGDQIAE"
     gene            complement(5540..6463)
                     /locus_tag="PLK64_03085"
     CDS             complement(5540..6463)
                     /locus_tag="PLK64_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HPV78754.1"
                     /translation="MSVLTLHPEVVDALATGRAVVALESTILAHGLPPGDNLRVGREI
                     EAAVRAAGAVPATIAVLDGQVRVGLDDAGLERLCDPSADILKLSVRDVGVALALHRDG
                     ATTVASTSALAHRAGISVFATGGLGGVHRGAAETFDVSADLGVIARTPVLVVCAGVKS
                     ILDVPGTLEYLETLSVPVLGYRTDAFPGFYLSDSGYAAPWRVDSPAQAAAIVRARELL
                     GGEEAGVVLANPLPLDRQVDPLLHDRLVADGLEMLAAEGIHGKDVTPALLAYFHEHSD
                     GESLRANVELVLANARLAAEVAVELAALRAQ"
     gene            6741..7346
                     /locus_tag="PLK64_03090"
     CDS             6741..7346
                     /locus_tag="PLK64_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78755.1"
                     /translation="MGRLARAYIVAAVAATVSLAGCSSTPEPTLPSFGPTPTATASSP
                     QTPAATASPTATATATAVGAMNFLEMPAGFTAEQRAVGLAYQEYWKLASRAYQTPREP
                     HPDLGKAITGEALVGLNGYVNKLVSTGRHLEGPAEFKVVTVSVSGATAKACGSMLDSS
                     HEIDDVTGESKEAIDPIIRLYSATFTQSGGVWRVATLKRGC"
     gene            7619..8479
                     /locus_tag="PLK64_03095"
     CDS             7619..8479
                     /locus_tag="PLK64_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78756.1"
                     /translation="MPASCWWESVKYDYGGFQQDNPSSWVAWYDDLTNPLASHSYASD
                     GNYTWGPRSMYQAAAASPNANALDLYSINCRDNATYCSGILMSYAGTPQVYDPGSCPV
                     PVTFRFFAGAPPQPLVDPQDLAQVARDYMVIPDPVAERNPKLDLTGGGTLVSLPTWFW
                     VTNPDAVGAATGGTRTIRAQVGPVWAEVVARTSGLSINSPAGGTSCSPDRAVRVYAPG
                     ADDSTGCTVAFDAASVRYADGYPVDLSTSWSATWTGSGGTGGALPGLSRTVTTNVPVA
                     ESQALVYSYR"
     gene            8719..9180
                     /locus_tag="PLK64_03100"
     CDS             8719..9180
                     /locus_tag="PLK64_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78757.1"
                     /translation="MATSTASAAATAVDKIDFPDLPSGLSAEQEAVGRAWQAYWRLAW
                     TVFRTPKEPNSDLGKVMTGRALGLFNGYVDKLKLEGRHLEGSTTFWVDSISVSDGTAT
                     GCGRLVDFSHEVDDVTGESKEPINPRVRVMSATFVKSGDLWRISSSTKGGC"
     gene            9262..10290
                     /locus_tag="PLK64_03105"
     CDS             9262..10290
                     /locus_tag="PLK64_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78758.1"
                     /translation="MRQIRAALICAVAAISLLVSPGTAQAGIEDASDGAGFGVRVGFS
                     GDGAPSGGGSTVVRMPASCWWTPINYMGFQQDNPSSWVEWYDTVVRELRDHAHAAIAY
                     FQMGPRSMYEAAAASPNANALDLYGIDCRDDATYCSGILMSYAGTPQVYDPGGCPVPV
                     THRFFAGATPQPLVDPEDLAQVARDYMAIPDPVAERNPKLDIAGGSTLVSLPTWFWVT
                     NPDAVGAATGGTRTIRAQAGPVWAEVVAQTSGLSISSPAGGTSCPPDRAVRVYAPGAD
                     DSTGCTVAFEAASVRYADGYPVDLSTSWSATWTGSGGTGGVLPGLSRTVTTNVPVAES
                     QALVYSYR"
     gene            10347..11564
                     /locus_tag="PLK64_03110"
     CDS             10347..11564
                     /locus_tag="PLK64_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPV78759.1"
                     /translation="MTAPVLDLALARLAVIEDMLLDFRRDVHRHPELSFAEHRTTANV
                     ADALTDAGVKVTLLPGSGLVADLGAEHAAYRIALRADLDALALPERTHLPYASVVPGV
                     AHACGHDVHVTVCLGAALALVSVQDHLVSSGLGVRVIFQPAEEVIPGGALPICESEWL
                     DGVDEIYAVHCDPTLDVGSIGLRVGPLTAAADRVTVRLSGSGGHTSRPQLTQDLVFAL
                     GKVITEVPAALGRRVDPRSSASLVWGTVEAGHAANVIPESGSVSGTLRVLDAGAWDNA
                     GALVEELVSSAVAPYGVQMAIEHIRGVPPVVNAPEAVDLLTDAAKRVGVDPVPTEQSM
                     GGEDFAWYLTKVPGAMARLGTRMPGGPTFDLHQGDLVVDEASVTHGARVLTAAVLSRR
                     LPGGVAVGDVPSR"
     gene            11696..12742
                     /locus_tag="PLK64_03115"
     CDS             11696..12742
                     /locus_tag="PLK64_03115"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPV78760.1"
                     /translation="MRHSIKAAALLTVAALGLAACSSSASTTTTTTAASTAGAPAAGA
                     LKVGMAFDTGGRGDGTFNDLAVKGLERAAKELGATAKELSPNADGTNRADLLRQLADG
                     GYSPIFGVGYNYGEPMDQVAKEYPKVQFVRIDGAPSTVANVAVMDFAEQEGSFLVGVA
                     AALKSKTHHVGFIGGNESVVINRFGAGFAQGAKAADPTIVVDDKRLAAADSGAGFGDV
                     PGAKVAAAAMYQNGADIVFTAAGGSFAGSFPAAKDAGKLVIGVDSDQFNTVSDAALKP
                     LILTSMIKRVDNAVFLSVQAFKTTGKLTSHSYNLKDEGVAYSTSGGFVDDIKSKLEDY
                     KAKIIAGTIVVNTK"
     gene            12839..14413
                     /locus_tag="PLK64_03120"
     CDS             12839..14413
                     /locus_tag="PLK64_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV78761.1"
                     /translation="MPTEVHPTPPSAQSSPAAAIIELRGITKRFPGVVANRDIDLVVR
                     AGEVHALVGENGAGKSTLMKVLYGMYHPEEGQILVNGTPTPFRGPNDAIAAGVGMVHQ
                     HFMLADNFTVLENIILGSEPQSRGRLDIDAARSRIDQISRDYGLDLDPDALVENLTVA
                     DRQRVEIAKVLFRGARVLILDEPTAVLVPQEVDELFEHLQEMTAEGLAIVFISHKLDE
                     VLKVADVITVIRRGTTVASVDPGSVTARQLAELMVGSELPKPDTRESTVTDHEQLHVT
                     RLALAAPSGKPILDDISFVVHRGEVAGFAGVEGNGQSELVAALLGLVKPDSGTISVDG
                     RDISNETTMGRRNAGIAVVPEDRHRQALLLASPLWENRLLGHTHDPRLFTSWGWLKKG
                     ATVSDTVRIMADYDVRAPGPETLVGSLSGGNQQKLIIGRELESDPSILIAAHPTRGVD
                     VGAQAAIWDRIRAARAKGLATVLISADLEELIGLSDVLYVIYKGRLVAKLDPADVTPQ
                     ILGTYMTGAANKEASA"
     gene            14410..>15540
                     /locus_tag="PLK64_03125"
     CDS             14410..>15540
                     /locus_tag="PLK64_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV78762.1"
                     /translation="MTRLRRVVLTGVLPATLALLVAVLVLAVVLLVLGVDPVKSLVAL
                     FDFGETPQAQANQMRAWVEGVVPLFLSGLAVSLGFRMGLFNIGVEGQYRIAALVAAWF
                     AATVPLPGALAIILTIIVAMLAGALYALIPAVLKVTRGVNEVITTIMLNSIVGGLIGY
                     LLRGPFRDPKLGETGTGRTADIRTDAWFPGFEDPFGWLGMLTPSRPLGGFIVVALIVG
                     VAVALGLNRTRFGFNLRASGSNPEAAATSGVQSSRMIVQAMLLSGGIAGLVGLPQILG
                     DVHAYGADSFFLGFGFSGIAVALLGRNNPLGIGLAAILFAFLNRSGPSLQNVEVPPSV
                     VSIAQGVIVLSVVIVNEVLRRRLLRMEDRKAAAQTPATPAAPT"
BASE COUNT         2480 a         5339 c         5307 g         2414 t
ORIGIN      
        1 cggtggtgcc ggcgacgttc ggatgggatg acatcggcga tttcgactcg ctggcggggc
       61 tgctcgccag tcacgacagt gatgccagtg acgacggtga cgcgagtgtc ggcgggctct
      121 cgatcgtcgg ggagtcgggg gatgtcctcg cgttggactc gacagggttg gtcgtcgccc
      181 gctcgggtcg gctggtggcc ctgatcggtg tcgaggacct cgttgtcgtc gacaccccgg
      241 acgcactcct catcgtgccg agggctcggg ctcaggaggt caaggggatc gtcgaccgac
      301 tggccgacct cggccgcgat cacctgcgct gagccacggg tgccctgagc cgctcagccg
      361 atgtcgcggc ggcggaagcc gaggacgccg acgatgcaca acccggctgc gaccgctgtc
      421 agcgcgatcg tggggggcca cgcgaacgcc tcgacgggca tccgcggaac gtgggtgaac
      481 ggcgcgagat ccatcagcca ctgcggcatc ccgaacacgc caccgaactc gccgacgacg
      541 aggaagaagg ccagcgcccc ccacccgatc gccgccgtcc atcggggaaa ccacccgatg
      601 gccgcaaccg cgacgcccac gcacacccag accgcaggga gccggatcag cgcggcgggc
      661 aggacccgac cggctccacc gagggtcccg ccgccggacg catcggccag aacggtgccg
      721 aggccgagcg cgaagaccag tgcggcggtg ccgagcaggg cgacgagcag gtggctcgcg
      781 gcataccgag tgcgcgagtc ggccgtggcg aggacgagct cggtatgccc ctgcgtctcc
      841 tccgcgcgcc agcgcagcac ggtcgtgacg ccgtatgccg cggcgatgat cgcgatgaag
      901 cccaattccg tggacagata aacatcggac aacaggccga cgccgccacc catcttgcgc
      961 aataggtcgg cgatcgccgg gtccgcgaac atgtccatcg cactcttggc gagcgacccg
     1021 atgaccaggc cgccgacgag catccccacc gtccagccga tcgtcgagct gcgaccgagc
     1081 cgccaggcca acccgaccgg cccggcgagg gtggcgccgg cgttggcccg accgcgccga
     1141 gcaggcagca gacccgcgcc gaggtcgcgt cgttgcagca acgcgaaggc ggccacgagg
     1201 cagacggcca gcaggcccac gccgaggaac acgacccagg aacggtccgc cccgaaggct
     1261 tcggccttgc tcgtccaccc gaccggcgag acccagccca accagccggg gacccccgtg
     1321 ccggcgtcgg cgatggcgcg cagcaggaac aacacgccga gggcgcccat cgtcagaccg
     1381 gcgcagccgc gagcggtgga ggcgagttgg gcggcgacgg ctgcgaggcc gacgaacgcc
     1441 aatcccgttg ccgcccagcc agctgcggcc gcgagtgagc cggccacgtc catgccgatg
     1501 gcggcatacc caaggccggt gagcacgcac gtgactagga ccgcggcggt cgccacggcc
     1561 accgcggcgg tgagcggagc ccggcgcccg accacgcccg ccccgacgag ctcgaagcgg
     1621 ccgtcctcct cctcggagcg agtgtggcga cgcacgacgg catacccgaa gatcgcgagg
     1681 agcactgccc cgaacatcgt cgtcttgacg acggcgaggg cgtcggggtc ggtcgggtcg
     1741 gagattgggc catagagcgc cacggccgac gggctggcga agatcgtctt gatgccggcg
     1801 tccatcgacg cgacggtcgg gtagagcgcg agggtcgcct tggcactgct caccgcgaag
     1861 atcgtgatcc cgaggatgcc gacgggcagc agcacccggt cgcgtcgcac ggccagtcgg
     1921 gcgagcgtcc ccgtgccgac caggctcgcg gtcatgacgc gtagctcctc atgaacagtt
     1981 cctcgagcgt gggcggctgg ctggtcaggg tctgtatgcc gtgtgccccg agcgcgctca
     2041 gcaccgcgcc gatgtggtcg gtatcggccg agagggtcac cctcgacccg tcgagcacga
     2101 catcgtgcac gcctgtcagc gcggccaatt cggctgcgct gggcgggtgt tcgagagttg
     2161 ccgtcactga ggtccgggtg agatggcgca actgagcgag gctgccggac tcgacggtgc
     2221 gtccggcccg gatgatgctc acccggtcac atagcgcctc gacctcggag aggatgtggc
     2281 tggagagcag gaccgtgcgc ccctcggtgc gcacctcgtc gacgtggtcg cggaagacgc
     2341 ggtccatcag cgggtcgagc cccgctgtcg gctcgtcgaa caacagcaac tcggcgtctg
     2401 aggacagtgc tgcgacaagg gcgaccttct gccggttgcc cttggagtag gcacgacatt
     2461 tcttggtcgg gtcgaggtcg aagcgggcga gcaggtcgtc ccgcctggcc gcgttgaggc
     2521 ccccgcgcag ccgggcgagc aggtcgatga cttcgccgcc ggtgaggttg ggccagaggg
     2581 cgacatcgcc cgggacgtag gcaatccgtc ggtgcaactg cgccacatcg gtccaagggt
     2641 cgccgcccag caatcgggcc tggccaccgt cggcgcgcaa caacccgagc aggacccgga
     2701 tcgtcgtcga cttgcccgcg ccgttgggtc cgaggaagcc gtggatctcg ccctgcgcga
     2761 cggtgaggtc caggccatca agggcgacgg tggtgccgaa ggactttcgc agcccgctga
     2821 tctcgatcgg agcgctcata gttccatggt tacaccacta ccgccggcct accccgactg
     2881 ccctcttgtg tgttcgccgc ggccgtcggc acacggcata cgagagtgac atcagtccca
     2941 tgaccgcggt ggtgagccgc cactaggctg aaacgcatgg ctgacgcgcg ctccgagtcc
     3001 gaccttccga tcaagccgat gtatgccgcg actgacctcg cggcattcga cgcagcgacc
     3061 cagttgggtg acgcaggcaa gtacccgttc acccgtggcg tctacccgtc gatgtatacc
     3121 ggccggccgt ggacgatgcg tcagtacgcc gggttcggga cggccaagga gtccaacgag
     3181 cgctaccacc aactcgtcgc ggcgggcacc ggcgggctgt cggtggcctt cgacctgccg
     3241 acccagatgg gctatgactc cgacgagtcg atctcctccg gcgaggtcgg caaggtcggt
     3301 gtggccatcg actcgatcga cgacatgcgg gtgctgttcg acgggctgcc gctggacaag
     3361 atctcgacgt cgatgacgat caacgcgccc ggctcgacgc tcctgctgct ctaccagctg
     3421 gtcgcggagg agcagggcat ctcgggcgac caactcaccg ggacgatcca gaacgacgtg
     3481 ctcaaggagt acatcgctcg cggcacctac atctacccgc cgaaggagtc gctgcgcctg
     3541 atcgccgaca tcttcgccta ctgccaggcc gagatgcccc ggtggaacac catctccatc
     3601 tcgggctatc acatggccga agcaggggcg acgcccgcgc aggagatcgc cttcacgctg
     3661 gccaacgcca aggagtatgt ccgggccgcg cagaaggcgg gcctgggcgt cgatgacttc
     3721 gcgccccgac tgtccttctt cttcgtggct cgcacgacgt tgctggagga gatcgcgaag
     3781 ttccgtgccg ccaggcggat ctgggccaag atcatgcgcg acgagttcgg cgccaagaac
     3841 cccaagagcc agatgctgcg cttccacacc cagacggctg gtgtgcagct gacggcacag
     3901 cagcccgagg tcaacctcat ccgggtggcg ctgcaagggc tgggcgccgt cctcggcggg
     3961 acccagtcgc tgcacacgaa ctcctacgac gaggcgatcg cgctgccgac gcagaaggcc
     4021 gctcggctgg ccctgcggac gcagcaggtc atcgcctacg agaccgacgt gtgcaagacc
     4081 gtcgacccgt tcgccggctc ctatgccatc gaggcgatga ccgacgagct cgaggtcgcc
     4141 atcgaggacc tcatccagca ggtcgaggac cgtggtggtg ctgtgcgggc catcgagcag
     4201 ggcttccaga agggtgagat cgagagctcg gcctatcggg tcgccctcga gatcgacagc
     4261 ggcgagcgca cggtcatcgg tgtcaacaag ttcaccgtcg acgtcgagga ggagtacgag
     4321 cccctgcgcg tcaacccggc catcgagtcc gagcagcgcg accggctggc caagctccgc
     4381 gccgagcgtg acaacgacgc ggtgacggcg gccctggagg cgctcaagga agcggctcgc
     4441 gggaccgaga acatcctcta cccgatgcgc gaggcgctgc gggcccgggc caccggcggc
     4501 gaggtcgctc acgcactgcg cgaggtctgg gggacctata tccccaccga ctcgttctga
     4561 tccgacgggc tcactgggcc agttgggcgg gctactcggc gatctgatcg ccgcgggccc
     4621 gaccggtgat gacggccgtc ccggcgcaga ctccggcagc caacgcggca gctggatccg
     4681 cgccggcgac ccacgccgcc aacagcccgg cgtcgaaggc gtcgccggcg ccggtcgtgt
     4741 ccggtgccgc tacgggctcg gccgcgacct cccagacctc gccgcctgcc ccgaaccagc
     4801 gcgcccccgc tgcgccgtcg gtcaccgcga cggcgtgcac gttggccagg atcgtcggca
     4861 cgcctccgag ggcgtcggcc tcgttggcgt tgggcagcag caggtcgaca ccgcggaccc
     4921 agtcgaggaa caccgccgca cccacctcgc tcaccaaggc tgcggcctgc gggtcgactg
     4981 atgtgctcca cccacgccgc cgcgcctcgc cgagcgctgc gaggcccgcc gcgcgtgagc
     5041 ccggatcgag caggacgtac cccgagaggt gcaggtgcgg catacccccg gggaaggcgg
     5101 ccacgacgtc gagggcaccg gtgacgtcgg tggagcgcaa ggccgcgttg gcgccgcgcg
     5161 agggcagcat ggtgcgctca cctgacgcgt cgacgaggac gacgaccagg ccggtgggca
     5221 ggtcgcggtc gacggcccag acgcagcgga ctccggcgcg ttcgagttcg gcccgagccg
     5281 tgcgcccggc ggagtcgtca cccacgcgcg cgacgagcgt gacgtctgct ccggcggtgc
     5341 cgagccaggc ggccgtgttg gcccccgccc cggctcgccg catgctcacc tgcgcgggcg
     5401 tgtccgtccc gtagcggatc ggaccgctcg gcaccgcaac gacatccatg ccgacgtcac
     5461 cgatgacgac gatcggccgc gcacctgccg cagtgctcac gacgcccatc cggtatgccg
     5521 cccgctcacc gctgacccgt cactgggcac gcagcgcggc gagctcgacg gcgacctcag
     5581 cggccagccg ggcgttggcg agcaccaatt cgacgttggc gcgcagggat tcgccatcgg
     5641 agtgctcgtg gaaataggca agcaaggccg gggtgacgtc cttgccgtga atgccttcgg
     5701 cggccagcat ctccaggccg tcggcgacga gccggtcgtg cagcaggggg tcgacctgtc
     5761 ggtcgagggg gagggggttg gccaggacca cgccggcttc ctcgccgccg aggagctccc
     5821 gggcccggac gatggccgcg gcctgggcgg gggagtcgac gcgccagggg gcggcatacc
     5881 cggagtcgga gaggtagaac cccggaaagg cgtcggtgcg gtagccgagc acggggaccg
     5941 acagggtctc gaggtattcc agggtgccgg gcacgtcgag gatcgatttg accccggcgc
     6001 agacgacgag gacgggggtg cgggcgatca ccccgaggtc agccgacacg tcgaaggtct
     6061 ctgccgcgcc acggtgcacg cctccgagcc cgccggtggc gaagaccgag atcccggcgc
     6121 ggtgggcgag cgcgctcgtc gaggcgacgg tggtggcgcc gtctcggtgc agggccagag
     6181 caacgccgac gtcgcggacg ctgagcttga ggatgtcggc ggaggggtca cacagtcgtt
     6241 ccagtccggc gtcgtcgagt ccgacgcgga cctggccgtc gaggacggcg atggtcgcgg
     6301 gtacggctcc tgccgcgcgc acggcggcct cgatctcgcg cccgacgcgc aggttgtcgc
     6361 ccggcggcag gccgtgggcg aggatcgtgc tctcgagggc gacgaccgcc cgtccggtgg
     6421 cgagggcatc gacgacttcg gggtgcaagg tcaggaccga catggcggca gcgtagacgt
     6481 gttggcgcgg cggcatacct gaccgtggcg cgccagctgc ggtcgggggg tgaggcgggg
     6541 cgcgcaaaga tgaccgtgcg agcgctatct ccgggggtca caggctgtga ggcccgcaaa
     6601 gatgaccgtg cgagcgctat ctctgggtct aaacccggcg tgggcccgca aagatgaccg
     6661 tgcgagcact atctccgggg ggtcggttag ggtcgtgggc gttggtcagg tggcgcgtca
     6721 aggtaagaca ggggaacccc atgggacggc ttgctcgggc atacatcgtc gctgcagtgg
     6781 cagcgaccgt ctcgcttgct gggtgcagtt caacgccgga gccgacgttg ccgagcttcg
     6841 gtcccacccc cacggcaaca gcgtcgtcac cccagactcc ggcggcgact gccagcccga
     6901 cggcgaccgc caccgcgaca gcagtcggcg cgatgaactt ccttgagatg ccagccggct
     6961 tcaccgctga gcaacgagcc gtcgggctgg cgtatcagga gtattggaag ctcgcctcgc
     7021 gggcgtacca aaccccgcgt gaaccgcacc ccgacctcgg caaggccatc accggcgagg
     7081 ccctcgtcgg gctcaacggc tacgtcaaca aactggtgag caccggacgc caccttgagg
     7141 ggcccgcgga gttcaaggtc gtcacggtct cggtttcagg agcgactgcc aaggcatgcg
     7201 gctcgatgct cgactcttcg catgagatcg atgacgtcac aggtgagtcg aaggaagcga
     7261 tcgatcccat catccgcctc tactcggcaa cgttcaccca gtcgggcggt gtgtggcggg
     7321 tcgctacgct caagcgaggc tgctgatcgc ctcgctggcg cccaatcgcg acgacggtac
     7381 cgtcgggtaa agtgccgagt cgtgaggtcc cgactcacag gtcgacaggg gaacgcgcat
     7441 gagactggtc agatcggcac tcatctgtgc cctggcagtg ttgggcctga ccgcgctgcc
     7501 cgcgaatgct tgggcaaaca atgggggtgg aggggaagcc tcgggcggcg gctacggcgt
     7561 cgcggtccgc ttctccgggg atggcgcccc gagcggcggg ggatccacgg tcgtgcggat
     7621 gcccgcatcg tgctggtggg agtcggtgaa gtacgactac ggaggattcc agcaggacaa
     7681 tccctcgtcg tgggttgcct ggtacgacga tctcaccaat ccgctggcca gtcattcgta
     7741 cgcgtccgac ggcaactaca cgtggggccc tcgctcgatg taccaggcgg ctgcggcaag
     7801 tcccaacgcg aacgccttgg acctgtacag catcaactgt cgggacaatg caacttactg
     7861 ttccggcatc ctcatgtcgt atgccggaac tccgcaggtc tacgacccgg ggagttgccc
     7921 ggtcccggtc accttccgct tcttcgctgg tgcccctcct cagccgctgg tcgacccgca
     7981 ggatctcgcc caggttgctc gggactacat ggtcatcccc gatcccgttg ctgaacgcaa
     8041 cccgaaactg gacctcaccg gcggcgggac cctggtctcc ctgccgacgt ggttctgggt
     8101 caccaacccc gacgctgtcg gggcggccac gggcggcacg cggacgatcc gggctcaggt
     8161 cgggccggtg tgggccgagg tcgtcgcgcg caccagcggg ctgagcatca attcgccggc
     8221 gggtgggacg agttgctcgc cggatcgtgc ggtccgtgtc tatgccccag gggccgacga
     8281 ctcgacgggc tgcacggtcg cgttcgatgc ggcgtcggtc cggtatgccg atgggtatcc
     8341 cgtcgatctg tcgacgtcgt ggtcggcgac gtggaccggg tccggtggca ctggaggtgc
     8401 cttgcccggc ctctcgcgga ccgtcacgac caacgtcccc gttgccgagt cgcaggccct
     8461 ggtctactcc taccgatgac cgtgcgagcg ctatctccgg ggtgggtggc gtggggctgg
     8521 tccgttaggg tctggggcgt tggtcaggtc ggcgcgtcaa ggcaggggag caccatggca
     8581 cggcttgcac gagcacatat cgctgcagtg gcagccgtcg tcgccctcgc tgggtgcacc
     8641 tcatcgccgg agccgaccct gccgagtttc ggtaccacgc ccacggcaac cgcgtcgtca
     8701 ccttcgactc cgacggccgt ggcgacatcc acggcctcag ccgccgcgac tgccgtcgac
     8761 aagatcgact tcccggacct accaagcggt ctctccgccg agcaggaggc cgtgggccgg
     8821 gcctggcagg cctactggag gctggcctgg acggtgttca ggactccgaa ggaaccaaac
     8881 tccgacttgg gcaaggtcat gactgggcgg gcgttgggtc tcttcaacgg ttacgtcgac
     8941 aaactcaagc tcgaagggcg ccatctggag ggctcgacaa ccttctgggt tgactcgatc
     9001 tccgtctcgg acggcaccgc caccgggtgc ggtcggctgg tcgacttttc ccatgaagtc
     9061 gacgacgtca ccggcgaatc gaaggaaccg atcaaccccc gggtgcgcgt catgtccgcc
     9121 acattcgtca agtctggcga tctctggcgg atctcgtcga gcaccaaggg cggctgttga
     9181 gcccaaccgg gtcgaagctc ctaggcccgg ttctcatgag gctggtaaaa tgcgcgaacg
     9241 tctcggcaca ggggagctag catgcgacag atcagggcgg cgcttatctg cgccgtagcg
     9301 gcaatctcgt tgctggtctc accgggaacg gcccaggcgg gcatagagga cgcgtcagac
     9361 ggcgcgggct tcggcgttcg agtcggtttc tccggagatg gcgcacccag cggcggtggg
     9421 tccacggtcg tgcggatgcc ggcgtcatgc tggtggactc ccatcaacta catgggcttc
     9481 caacaggaca acccctcgtc gtgggttgag tggtacgaca ccgtcgtccg ggagctgcgc
     9541 gatcatgcgc acgctgcgat tgcctacttc cagatgggcc ctcgctcgat gtacgaggcg
     9601 gctgcggcca gcccgaacgc gaacgccttg gacctgtacg gcatcgactg tcgggacgat
     9661 gccacctatt gctccggcat cctcatgtcg tatgccggaa ctccgcaggt ctacgatccg
     9721 ggcggttgcc cagtcccggt gacgcaccgc ttcttcgcgg gcgcaactcc acagccgctc
     9781 gttgaccccg aggaccttgc ccaggtggcc cgcgactaca tggcgattcc cgatcctgtt
     9841 gctgaacgca acccgaaact ggacatcgcc gggggcagca ccctggtctc cctgccgacg
     9901 tggttctggg tgactaaccc cgacgccgtg ggggcggcca ccggcgggac gcggacgatc
     9961 cgggctcagg ccgggccggt gtgggccgag gtcgtcgcac agaccagtgg gctgagcatc
    10021 agttcgccgg cgggtgggac gagttgtccc ccagatcgtg cggtccgcgt ctatgcccct
    10081 ggtgccgacg actcgaccgg ctgcacggtc gcgttcgagg cggcgtcggt gcggtatgcc
    10141 gatgggtatc ccgtcgatct atcgacgtcg tggtcggcga cgtggaccgg gtccggtggc
    10201 accggcgggg tcttgcccgg cctctcgcgg accgtcacga ccaacgtccc cgttgccgag
    10261 tcgcaggcgt tggtctactc ctaccgatga ccgtgtctgc tgaccgtgtc tagtgcgcgc
    10321 tcgcgcgctc actagactgc cccctcgtga ccgccccggt gctcgacctc gcgcttgcgc
    10381 gcctcgccgt gatcgaggac atgctccttg acttccggcg cgacgtccat cgccaccctg
    10441 agttgtcctt cgccgaacac cgcacgaccg cgaacgtggc cgatgcgctg accgacgcgg
    10501 gtgtcaaggt caccttgctg cccgggtcgg ggctggtcgc cgacctggga gccgagcacg
    10561 cggcataccg gatcgccttg cgagccgacc tcgatgcgtt ggcgctgccg gagcggaccc
    10621 acctgcccta cgcctcggtg gtgcccgggg tggcccacgc gtgcggtcac gacgtccacg
    10681 tcacggtctg cctgggcgcg gctctggccc tcgtctcggt ccaggaccac ttggtcagca
    10741 gcgggctggg cgttcgggtg atcttccaac ccgccgagga agtcatcccc ggtggtgcgc
    10801 tgccgatctg cgagtcggaa tggctcgacg gcgtcgacga gatctatgcc gtccactgtg
    10861 accccaccct cgacgtcggg agcatcggcc tgcgcgtcgg cccgctgacc gctgcggccg
    10921 accgggtgac cgttcggctc tccggctccg gtggtcacac gtcgcggccc caactcactc
    10981 aggacctggt cttcgcgctc ggcaaggtca tcaccgaagt ccctgccgcg ctcggccgac
    11041 gggttgaccc gcgctcgtcc gcgagcctgg tgtgggggac ggtcgaagcc gggcacgcgg
    11101 ccaacgtcat ccccgagagc ggcagcgtgt ccgggaccct ccgggtgctg gacgcagggg
    11161 cgtgggacaa cgccggtgcc ctagtcgagg aactcgtctc atccgcggtc gcgccgtacg
    11221 gcgtgcagat ggccatcgaa cacatccgcg gcgtcccgcc ggtcgtcaat gcccccgaag
    11281 cggtggacct gctcacggat gcggccaagc gcgttggcgt cgacccggtc ccgacggagc
    11341 agtcgatggg cggggaggac ttcgcctggt acctcaccaa ggtgcccggt gcgatggccc
    11401 gactgggcac gcgtatgccg ggtggtccga ccttcgacct gcaccaaggg gaccttgtgg
    11461 tggacgaggc cagcgtgacc catggggcgc gtgtgctcac tgcggcggtg ctgtcgcgac
    11521 ggcttccggg aggcgtcgcc gtgggcgacg ttcccagtcg gtaacgaacg ttccgagtcg
    11581 gtaacaaacc cgcacggtgt agggcagtcc tcgccggtgc ctgtaggcgt ggcgctacgc
    11641 tcgcgatatc cgcgcgccgg caaccccggc gccttcggtc atggaggaga tacctttgcg
    11701 tcactcaatc aaggctgcag cgctgctcac tgtcgctgcg ctcggactcg cagcctgcag
    11761 cagcagcgcg agcaccacga caaccacgac agccgcctcg acggcagggg cccctgcggc
    11821 gggcgccctc aaggtcggca tggccttcga caccggtggg cgcggcgacg gcaccttcaa
    11881 cgacctcgcg gtcaagggtc ttgagcgggc tgccaaggag cttggtgcca cggccaagga
    11941 actgagcccc aacgccgacg gcaccaaccg ggcagacctg ttgcgccagc tggccgatgg
    12001 cggctacagc ccgatcttcg gcgttggcta caactacggc gagcccatgg accaggtggc
    12061 caaggaatac ccgaaggtcc agttcgtccg catcgacgga gccccgtcaa cggtggcgaa
    12121 cgtggcggtc atggacttcg ctgagcagga gggttccttc ctcgtcggtg tggctgcggc
    12181 cctgaagtcc aagacgcacc acgtcggctt catcggcggc aacgaatccg tcgtcatcaa
    12241 ccgcttcggt gcgggcttcg cacagggcgc caaggccgcc gaccccacga tcgtcgtcga
    12301 tgacaagcgt ctcgccgcgg ccgactcggg tgccggtttc ggcgacgtcc cgggagccaa
    12361 ggtcgctgct gcggccatgt accagaacgg tgccgacatc gtcttcaccg ccgctggcgg
    12421 ctcgttcgca gggtcgttcc ctgcggccaa ggacgccggc aaactcgtta tcggcgtcga
    12481 cagcgaccag ttcaacaccg tgtccgacgc ggcgctgaag ccgctcatcc tcacctcgat
    12541 gatcaagcgc gtcgacaacg ccgtcttcct cagcgtccag gccttcaaga ccaccgggaa
    12601 gctcacgtcg cacagctaca acctcaagga cgaaggggtc gcctactcga cgtccggtgg
    12661 cttcgtcgac gacatcaagt ccaagctcga ggactacaag gccaagatca tcgcgggcac
    12721 gatcgtcgtc aacaccaagt agttcgctgt cgatcggaca aggacgtccg cctgacacgt
    12781 acccacgtgg ggggcccggc acctgccggg ccccccacgg ctatggaagg atgcgcgcat
    12841 gcccaccgag gtgcacccga cgcccccgtc tgcccagtcc tcgccggccg cagcgatcat
    12901 cgagctgcga ggcatcacca agcgattccc cggcgtcgtc gcgaaccggg acatcgacct
    12961 ggtcgtgcgc gccggagagg tccacgcgct cgtcggggag aacggcgccg gtaagtcgac
    13021 gctgatgaag gtcctttacg ggatgtacca cccggaggaa gggcagatcc tcgtcaacgg
    13081 cactccgaca ccgttccgcg ggcccaacga cgccattgcc gcaggggtcg gcatggtgca
    13141 tcaacacttc atgctggccg acaacttcac cgtcctggag aacatcatcc tgggcagcga
    13201 accccaatcc cgggggcggc tggacatcga cgctgcccgc agcaggattg accagatctc
    13261 gcgggactac ggcctggatc ttgacccgga cgcactcgtc gagaatctca ccgtggccga
    13321 ccggcaacgc gtcgaaatcg ccaaggtgct cttccgcggt gcgcgagtcc tcatcctcga
    13381 cgagcccacc gccgtcctcg tgccgcaaga ggtcgacgag ctcttcgagc atcttcagga
    13441 gatgacggct gagggcctgg cgatcgtctt catctcgcac aaactcgacg aggtgctcaa
    13501 ggtggccgac gtcatcactg tcatccggcg tggaacgacg gtcgccagcg tcgatcccgg
    13561 gtcagtgacg gcccgtcagc tcgccgagct catggtcggc tccgaactgc ccaaaccgga
    13621 tacccgcgag tccaccgtga ccgaccacga gcaactgcac gtcacccggc tcgcgcttgc
    13681 cgcgccgtcg ggtaagccga tcctcgacga catctccttc gtcgtccatc gcggagaggt
    13741 cgccgggttt gccggtgtcg aggggaacgg ccagagcgag ctcgtcgccg ccctactcgg
    13801 gctcgtcaag cccgacagcg gaacgatctc ggtcgacggt cgcgacatca gtaacgagac
    13861 gacgatgggg cgacgcaacg ccggcatcgc ggtcgttccc gaggaccgcc accgtcaggc
    13921 gctccttctc gcctcgccgc tgtgggagaa ccggctcctc gggcacacgc acgacccgcg
    13981 cttgttcacc tcgtggggct ggctcaagaa gggcgccacc gtctctgaca cggtcaggat
    14041 catggcggac tacgacgtgc gggcacccgg gccggagact ctcgtcggat cgctctccgg
    14101 cggcaaccag cagaaactca tcatcggtcg cgagcttgag tccgacccgt ccatcctcat
    14161 cgcggcccac cccacccgcg gcgtcgacgt cggggcccag gcggcgatct gggatcgcat
    14221 cagggcggcg cgagccaaag gcctggccac ggttctcatc agcgccgacc ttgaggagct
    14281 catcggcctg tccgacgtcc tctacgtcat ctacaaggga aggctcgtcg ccaagctaga
    14341 cccggctgac gtgacacccc agatcctcgg cacctatatg accggggctg cgaacaagga
    14401 ggcctcggcg tgacccgctt gcgccgggta gtgctcaccg gagtcctccc cgccaccctg
    14461 gcgttgctcg tggcggtcct cgtgctggcc gtcgtgctcc tggtgctcgg cgtcgaccca
    14521 gtgaagtcgt tggtggccct gttcgacttc ggcgagactc cgcaagctca ggccaaccag
    14581 atgcgcgctt gggtggaggg cgtcgtcccg ctcttcctct cgggcctcgc ggtgagcctc
    14641 ggcttccgca tggggctgtt caacatcggt gtggaagggc agtaccgcat tgctgcgctg
    14701 gtggcggcgt ggttcgctgc gaccgtccca ctccccggcg ctctcgccat catcctgacg
    14761 atcatcgtcg ccatgctcgc cggcgcgctc tatgccctga tccctgcggt gctcaaggtg
    14821 acgcggggag tcaacgaggt catcacgacg atcatgctca acagcatcgt cggaggcctc
    14881 atcggctatc tgctgcgcgg cccgttccgc gacccgaagc tcggcgaaac ggggaccgga
    14941 cgaaccgccg acatccggac cgacgcctgg ttccccggtt tcgaggaccc gttcgggtgg
    15001 ctcggcatgc tgaccccgag ccgaccgctc ggcggcttca tcgtcgtggc gctcatcgtg
    15061 ggcgtcgcag tggccctcgg cctgaaccgc acccgcttcg gattcaacct gcgggcgtct
    15121 ggaagcaacc ccgaggcggc cgccacgagc ggcgtccagt cgtcccggat gatcgtgcag
    15181 gccatgctcc tgtccggtgg catagccggg ctggtgggac tgccccagat cctcggcgat
    15241 gtccacgcct acggtgccga cagcttcttc ctcgggttcg gcttctccgg tatcgccgtg
    15301 gcgctcctcg gccgcaacaa ccctctgggc atcggcttgg cggccattct tttcgccttt
    15361 ctcaaccggt ccgggccttc ccttcagaat gtcgaggtcc ccccatcggt ggtgtcgatc
    15421 gcccagggcg tcatcgtcct gtcggtcgtc atcgtcaacg aggtccttcg tcgcaggttg
    15481 ctgcgcatgg aggaccgcaa ggctgccgcg cagacccccg caactcccgc ggcccccacc
//
LOCUS       DAOMZY010000031        15433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_3457_length_15433_cov_6.636364, whole genome shotgun sequence.
ACCESSION   DAOMZY010000031 DAOMZY010000000
VERSION     DAOMZY010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 15433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15433
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3457_length_15433_cov_6.636364"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2029)
                     /locus_tag="PLK64_03130"
     CDS             complement(<1..2029)
                     /locus_tag="PLK64_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPV78763.1"
                     /translation="MTTAHDLPQAPAGADDALAGELAIEQAHVDRVYAELDKASQRAR
                     LVAVEGLARGRTERTGEARDEELTGLFERDALVFHAARRQHALDTQYEGLVFGRLDLD
                     HPVAGRDSEALEREVRYIGRLGVRDDDYEPLVMDWRAPAAAPFYRATPVEPMGVLRRR
                     VLRCRGPVVIGAEDDLMVPEAPDGLVILGDGALMAALTRTRGARMRDIVATIQAHQDE
                     AIRAPGRGVTEITGGPGTGKTVVALHRAAYLLYADRRRYESGGILVVGPSAAYTAYIE
                     RVLPSLGEDSVALRALGDIVDAMSTDRHDTPAAAAVKGSLRMRELMARVARGLPDGVP
                     TELRTFVAGYAVRLDSTVLQRIRAHVLRQHPHNLGVEAARQTLAETALRLVGHPDRDD
                     FLGRFSDHIEVDAFLSAWWPQLDPREVLLWLTDRQRLDACAAGLLGPAEVDAVHASWM
                     EALETGTWSIADVALVDELAVRLGPEQEQPVEERGFFEIENLDELLEGATGGSFRPEE
                     VEPAYSTTPSDPRDRLLVGRIGRVADYAHVLVDEAQDLSPMQWRMLGRRGRRASWTVV
                     GDAAQSAWPDPVESLVARAEAWGGQEHRLFHMGTNYRNAREIFDYAAAVVRTEVPDAD
                     IPEAVRETGIPPREARLGERPLESIEAEAQRLLTEVDGSVAIITPRRYAAAL"
     gene            2197..3489
                     /gene="purD"
                     /locus_tag="PLK64_03135"
     CDS             2197..3489
                     /gene="purD"
                     /locus_tag="PLK64_03135"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPV78764.1"
                     /translation="MKVLVIGSGAREHALVRGLSADPDVSAVVAAPGNPGIDLIAQCE
                     PLADVCDAAAVLAVAARHTVDLVVVGPEAPLVAGVSDALREAGYAVYGPSAAAAQLEG
                     SKSFAKEVMASAGVPTALAHVCTTMAEVESALDALGAPHVVKDDGLAAGKGVVVTDDR
                     EAALAHARACLAKPDGQLVVEEFLDGPEVSVFCICDGTSVRALSPAQDFKRVGDGDLG
                     PNTGGMGAYSPLPWAPPHLSDWVIEHIAQPTIDEMHRRGTPFVGTLYVGLAMTSRGPR
                     VVEFNARFGDPDGQVSLARLVTPLGTLLYAAATGRLDDADDLHHRPESAVVVVLAAEG
                     YPAAPVPGGTIEGLDAAAEVPGAWVLHAGTRRAEDGRIVAAGGRVLDVMAVGADLASA
                     RDTAYRAVSCLELAGGHHRSDIALAAARGEIHIPGSDT"
     gene            3486..4406
                     /locus_tag="PLK64_03140"
     CDS             3486..4406
                     /locus_tag="PLK64_03140"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPV78765.1"
                     /translation="MSDSTALVLPGYEHIYSGKVRDLYAPLDPVTGERRTDQVLLVAS
                     DRISAFDYVLDTPIPDKGRVLTQLSLWWFDRLVGLENHVVSTDVPAAVAGRAVLVRRL
                     EMIPVECIARAYLTGGGLSEYRQSGAVSGVPLPAGLEDGSRLPVPIFTPSTKAAIGQH
                     DEPMTIEEVQSAVGTQVAARIDVLTRRILARGNEIAGANGIIIADTKVEFGIDRSRED
                     DSGGPVIVLADEVLTPDSSRFWPADQWQPGRSQPSFDKQFVRDWLTSPESGWDRSSGE
                     APPPLPEHVVEATRARYLEAYERITGNTLP"
     gene            complement(4463..5656)
                     /locus_tag="PLK64_03145"
     CDS             complement(4463..5656)
                     /locus_tag="PLK64_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012630880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPV78766.1"
                     /translation="MRDIGVGVISLGWMGRLHSTSYLAAPLRYSDLGVRTRLVHAADP
                     VPEAREYAAEQLGFARASADYRELLADPEVDVVSICSPNFLHKEIALAAAAAGKPFWI
                     EKPMGVSADESADIARAAAAAGVVTSVGFNYRHAPAVARARQLIRDGALGRITNIRVW
                     LIADYASAPEGPLTWRYDRDRAGSGVVGDLMSHGLDLAQFLVGRVASVTALSSTFITE
                     RPIPTKVGVGHGGFEIGDERGPVGNEDYVGILARFDNGVVGTLESSRVSIGPRSEYAV
                     EVYGTGGSLRWNFEKINELQTCLVSATGNGPEHGYTTSLAGPQHGDFARFQPGPGVPM
                     GFDDLKVVEAQLFLRSVLTGQQLAPSAADGWSAAAAADAAVESAADGQWHAVPEVEGI
                     TTYDA"
     gene            complement(5699..7597)
                     /gene="iolD"
                     /locus_tag="PLK64_03150"
     CDS             complement(5699..7597)
                     /gene="iolD"
                     /locus_tag="PLK64_03150"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HPV78767.1"
                     /translation="MTETVRLTGAQALVRFLANQHSERDGVQQRLVTGMFGIFGHGNV
                     AGVGQALLQDQTAADAGEPGEHLPYFLVRNEQGAVHAATAYSRTKNRLQTMAITTSIG
                     PGATNMVTGAALATTNRIPVLLLPSDQFATRYPDPVLQQLEDPGTLDVSVNDVFRPVS
                     RFFDRINRPEQLIPSLLGAMRVLTDPADTGAVTICLPQDVQAEAFDWPVALFRPRVWH
                     IGRPAPEAAALARAVEVIRSAKRPLVVAGGGVVYADGSEQLRAFATATGIPVADTHAG
                     KGAINWDHPSAVGGIGSTGTSAANALARQADVVIGIGTRYSDFTTASHTIFANPDVRF
                     VNINTLGFDAAKHSASMLVADAREALTALTGALDGWSVDAAYRQEAVDLDAAWQRAVE
                     ECYHRDQTPLPAQTEVFGALNELMGPEDVVINAAGSMPGDLQCLWRASTPVQYHVEYA
                     YSCMGYEIPAALGAKLALGDGHEVIAIVGDGTYQMLPQEIATIVSEGVKVIIVLLQNH
                     GFASIGGLSESRGSQRFGTNYRMRNRTSGRLDGEHVPFDLAANAASWGADVLRCATIA
                     DFRANYAAAAASDHTTVLYIETDLYGPNPPGSAWWDVPVSQVSDLQSTQTAYTEYVRE
                     QAAQRHYL"
     gene            complement(7661..8731)
                     /locus_tag="PLK64_03155"
     CDS             complement(7661..8731)
                     /locus_tag="PLK64_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HPV78768.1"
                     /translation="MSDNASPLQTMTQTTTTALWNDSADPRELAQSIEWGAVGATCNP
                     VIAVTCVKADLPRWSARMLEIAEQRPTASESQIGWQVVEEISIEAARLLEPAFEKFKG
                     HNGRLSMQTDPRLHRDAVALADQAERFSTLAPNVIVKIPATHKGIAAIEDATARGVSM
                     NVTVSFTVPQAVAAAEAIERGLAAREAAGHDVSHMGPVVTIMVGRLDDWLKDVVKRDG
                     IDIDPSYLEWAGVAAFKHAYAIFQERGFRSRLLSAAFRNQLQWSEFVGGDVVISPPFG
                     WQQKIQASGADAAPRMDIPVDPTILDALLQIPEFRRAYEPDGMTTEEFDEFGATRKTL
                     RQFLAADEELDHLVREVILPAP"
     gene            complement(8917..9795)
                     /locus_tag="PLK64_03160"
     CDS             complement(8917..9795)
                     /locus_tag="PLK64_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPV78769.1"
                     /translation="MKIAQDPTPFHHDYRLLDFPRVVADLGYEHIQLTPHRDLIPFFR
                     HPKADDALVRAFKKACDDAGVGIASVLPVQRWSGPDEVAREAAVRNWKRIVQITVDLG
                     VSVIGTEFSGRPERSEESEAAFYKSMEELLPIIEREGIDVLIDPHPDDFVEDGLEAIR
                     VIRGLNSPNVGMVFVACHSYHMGHPMREIMREAGDLIRLVHVADTMDHHRSHGLRYIS
                     NPPGNTARVHQHLKIGDGDLDWDDFFGGLGEIGFYDRPGTVMCNSVFAEDETAGDMAR
                     FQLATMQSYVAKHHAG"
     gene            complement(9890..10627)
                     /locus_tag="PLK64_03165"
     CDS             complement(9890..10627)
                     /locus_tag="PLK64_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPV78770.1"
                     /translation="MHQIPVMIDRASPVPLYHQLAEQLTAAVEDGTLQPGDPFENELA
                     LADRLDLSRPTVRRAIAELVSRGLLVRRRGVGTAVASEVIHRRDELTSLFEDMERKGK
                     RPITRVLDIDRHSVTPRAAAALNLPQDTPLFYVERLRLAGDIPTALMHNWLPPQFADI
                     TEEELASGSLYAVLRARGVRPVVAHQTIGARRPRSAERKRLDLAPGDPLLTMTRRAYD
                     AAGTAIEFGDHCYRFDQYAFDVTVHER"
     gene            10776..11735
                     /gene="iolC"
                     /locus_tag="PLK64_03170"
     CDS             10776..11735
                     /gene="iolC"
                     /locus_tag="PLK64_03170"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HPV78771.1"
                     /translation="MMAGPSFDVLCIGRVGVDLYPMEVGVGLEDVRTFGKFLGGSAGN
                     VAVAAARYGHSAAIISRTGDDPFGRFVATALGDLGVNTDYLGIDPWLPTPVTFCEIFP
                     PDHFPLYFYRYPQAPDLRVAPEQIPPVAVADSRVFWTTLTGLSAEPSRTAHATAWHLR
                     GRTAHTVLDLDYRPMFWVHESDASRAASDALEQSTVAIGNLEECRVAVGETDPDRAAR
                     ALLDRGVDLAIVKQGPKGVLAMTADERVEAPPVWVEVVNGLGAGDAFGGAVCHGLLNG
                     WDLAETISFASAAGAHVAGRLECATAMPDEAEVRALQARAGRS"
     gene            11774..12622
                     /locus_tag="PLK64_03175"
     CDS             11774..12622
                     /locus_tag="PLK64_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPV78772.1"
                     /translation="MLEPDGIPAAWAARRAGAVPADGRLLVIAADHPARGALGVRDDR
                     RAMASRSDLLQRLAIAVSRPGVDGVLGTPDILEDLLLAGLLDGLLVYGSMNRAGLQGA
                     VFELDDRFTAYDAATIERLGLDGGKMLARVAYDDPATAPTLEACGRAVTDLAERGKIA
                     LVEPFLSSRSADGWVRNLLDPDSVITSVAIASGLGATSAHTWLKLPVVPEMERVLDST
                     TLPTLLLGGDPTNEPAQTYATWGRALACPPAVGLVVGRALLYPPGGDVAAAVDVAADL
                     VHGGAR"
     gene            12619..13500
                     /gene="iolB"
                     /locus_tag="PLK64_03180"
     CDS             12619..13500
                     /gene="iolB"
                     /locus_tag="PLK64_03180"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HPV78773.1"
                     /translation="MSDWLRPAGSSGDAEFDVRIAPGDPGWSHTGLYAVTLAAGESRT
                     VDTGDCEWIVLPLSGGSAVVEVGERVYFLTGRAHVFAGPTDLVYVPRDSRFTVTSTDG
                     GRIAFPFAKASRAFPVCRVDSSQVPVEMRGTGSSSRQVQNFGTPGVLEADSIIACEVI
                     TPAGNWSSYPPHKHDEDRDGVESALEEIYYVEVQREGGDGSAAGIAYLRNYGTESAPI
                     DTLAEVRSGDVVLIPHGWHGPAMAPPGYDLYYLNVMAGPGETRAWLICDDPAHAWVRG
                     AWVDAPVDARLPFTQVP"
     gene            13505..14509
                     /locus_tag="PLK64_03185"
     CDS             13505..14509
                     /locus_tag="PLK64_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPV78774.1"
                     /translation="MRIGIVGVGRIGSFHAQTLAADPTVTDLVLLDPSATAAAAVASQ
                     LGATVAPDLDALLRRDLDALLVTSATHTHAEVLLAGVRAGLPTFCEKPVAASLDETIE
                     LARLEAASGVPIQVGFQRRFDTAYGRLRAAIASGELGFVHTIRAATHDQSPPHPGYLP
                     TSGGLFRDAAIHDYDVIRFVTGREVAQVYAAGANKGQKFFTEAGDVDTAAALLTLDDD
                     TLVVTSVTRYNRAGHDIRMEVHGSLGALAAGLDDRYPMTSAEPGARFPAGPAHQSFLE
                     RFAAAYRAEMTEFVQVAAGAVPSRCTVADALEAFRVAEACGISRREGRPVALAEIPGA
                     "
     gene            14620..>15433
                     /locus_tag="PLK64_03190"
     CDS             14620..>15433
                     /locus_tag="PLK64_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPV78775.1"
                     /translation="MSTPRRITHLIGHSEWDGTAERTGDVFNPATGEVTGKVDFASKA
                     LVDEVVANAAVAAAEWGRASLAKRTQVLFAFRQLLNDNKERVGALITAEHGKVTSDAI
                     GEVTRGLEIAEFACGIPHLLKGGFSENVSTNVDAYSILQPLGVVGVISPFNFPAMVPM
                     WFVPIAIACGNAVVIKPSEKDPSAVNAVAALWREAGLPDGVMSVVHGDKEAVDALLDN
                     TTVKAISFVGSTPIARYVYEKGTANGKRVQALGGAKNHMLVLPDADLDLAADA"
BASE COUNT         2211 a         5313 c         5501 g         2408 t
ORIGIN      
        1 ccagcgctgc ggcataccgg cggggggtga tgatcgcgac gctgccgtcg acctcggtca
       61 gcagtcgttg agcctcggcc tcgatcgact ccaggggccg ctcgccgagg cgcgcctcac
      121 gcggaggtat gccggtctcg cgcaccgcct cggggatgtc ggcgtcaggc acctcggtcc
      181 gcacgacggc tgccgcgtag tcgaagatct cgcgggcgtt gcggtagttg gtgcccatgt
      241 ggaagagccg gtgctcctgc ccaccccagg cctcggcacg ggcgacgagc gactcgaccg
      301 ggtcgggcca cgcgctctgg gcggcatcgc cgacgacggt ccaggaagcc cgtcggccgc
      361 gccgcccgag catccgccac tgcatcggcg acaggtcctg cgcctcgtcg acgagcacgt
      421 gggcatagtc ggcgacccgt ccgatgcgtc cgacgagcag ccgatcgcgc ggatctgacg
      481 gagtcgtcga gtaggccggc tcgacctcct ccggacggaa cgacccgccc gtggcgcctt
      541 cgaggagttc gtcgaggttc tcgatctcga agaagccgcg ctcctcgacg ggttgctcct
      601 gctcagggcc taggcgcacc gcgagttcgt cgacgagcgc gacgtcggcg atcgaccagg
      661 tcccggtctc aagggcctcc atccaggagg catggaccgc gtcgacctcg gcggggccaa
      721 ggagcccggc cgcgcaggcg tccagacgct ggcgatcggt cagccagagg agcacctcgc
      781 gcgggtccag ttgcggccac caggccgaga ggaatgcgtc gacctcgatg tggtcgctga
      841 agcggccgag gaagtcgtcg cggtcggggt gcccgacgag ccgcagggcc gtctcggcga
      901 gggtctggcg ggccgcctcg accccgaggt tgtgcgggtg ttggcgcagg acgtgcgcgc
      961 ggatccgttg caggacagtc gagtccaggc gcacggcata cccggcgacg aaggtgcgca
     1021 gctcggtggg gacgccgtcg ggcaggccgc gcgccacccg cgccatgagc tcgcgcatcc
     1081 gcagcgaccc cttgaccgca gccgcggccg gggtgtcgtg ccggtcggtc gacatcgcgt
     1141 cgacgatgtc gcccagggcg cgcagcgcga cgctgtcctc accgagggag ggcaggaccc
     1201 gctcgatgta tgccgtgtag gccgccgagg gcccgaccac gaggatgccg ccgctctcgt
     1261 aacgccgccg gtcggcatac aacaggtatg ccgcgcggtg cagcgcgacg acggtcttgc
     1321 cggtgccggg gcctccggtg atctcggtga cgccgcgccc gggagcccgg atcgcctcgt
     1381 cctggtgggc ctggatcgtc gcgacgatgt cgcgcatccg ggcgccgcgg gtgcgggtga
     1441 gcgccgccat gagcgcgccg tccccgagga tgaccaaccc gtcaggggcc tcggggacca
     1501 tgaggtcgtc ctcggcgccg atgacgacgg gcccgcggca gcgcaggaca cgccgccgca
     1561 ggacacccat gggctcgacg ggcgtcgccc gatagaacgg agcggcggcg ggtgcgcgcc
     1621 agtccatgac gagcggctcg tagtcgtcgt cgcggacccc gagtcgcccg atgtaacgga
     1681 cctcgcgttc gagcgcctcg ctgtcacggc cggccacggg gtggtcgagg tccaggcggc
     1741 cgaagaccaa cccttcgtac tgagtgtcca gggcatgctg acgacgagcg gcgtggaaga
     1801 cgagggcgtc gcgttcgaac agcccggtga gctcctcgtc ccgggcctcg ccggtgcgct
     1861 cggtgcggcc acgagccagg ccttcgacgg ccacgagtcg cgcgcgctgg gacgccttgt
     1921 cgagttcggc gtaaacacgg tcgacgtggg cctgctcgat cgcaagctct cccgcgaggg
     1981 cgtcgtcggc ccccgcgggc gcttggggca ggtcgtgtgc cgtcgtcatc gagcacccgt
     2041 cctggtcacg gcgtccatcc ggtcgcaggt ttccgtcccg tcgtatcgag aggccggtcg
     2101 cccggccacg gaacgcacaa gtctagtgca gcggcggccg ctcgcgcgcc cgcccagccc
     2161 gggcgagacg gcggcccggg gccctagggt tggggtgtga aggtcctcgt catcgggtcc
     2221 ggtgcccgcg aacacgccct cgtccgcggc ctgagcgccg atcccgacgt atcggccgtc
     2281 gtcgccgccc cgggcaaccc cgggatcgac ctcatcgccc agtgcgagcc gctcgccgac
     2341 gtgtgcgatg ccgccgctgt gctcgcggtg gctgcgcggc ataccgtcga tctggtcgtc
     2401 gtgggccccg aggcgcccct ggtcgcggga gtctccgacg ccttgcgcga ggcggggtat
     2461 gccgtctacg gcccgtccgc cgcggcggcc cagttggagg gcagcaagtc cttcgccaag
     2521 gaggtcatgg cctcggcagg ggtgcccacc gcattggccc atgtgtgcac gacgatggcc
     2581 gaggtcgagt ccgccctcga tgcgctcggg gcgccgcacg tcgtcaagga cgacggcctg
     2641 gccgccggca agggcgtcgt cgtcaccgac gaccgcgagg ccgccctggc ccacgcgcgc
     2701 gcctgcctgg ccaagccgga tggtcaactg gtcgtcgagg agttcctcga cgggcccgag
     2761 gtgtcggtct tctgcatctg cgacggcacc agcgtgcgag cgttgtcacc cgcgcaagac
     2821 ttcaagcgag tcggcgacgg tgacctcggg cccaacaccg gtggcatggg cgcctattcg
     2881 ccattgccct gggcgccgcc gcacctgtcc gactgggtca tcgagcacat cgcccagccg
     2941 acgatcgacg agatgcaccg ccgggggacc ccgtttgtcg gcaccctcta cgtcggcctg
     3001 gcgatgacgt cgcgcggccc acgggtggtg gagttcaacg cgcggttcgg cgaccccgac
     3061 gggcaggtct cgctcgctcg gttggtcacg cccctgggca cgctcttgta tgccgccgcg
     3121 accggccgcc tcgacgacgc ggacgacctg caccaccgac ccgagtcggc ggtcgtcgtc
     3181 gtgctcgctg cggagggcta ccccgccgcg ccggtgcccg gcggcacgat cgagggcctc
     3241 gatgcggcgg cggaggtgcc cggggcctgg gtgcttcacg cgggcacgcg ccgggccgag
     3301 gacggccgga tcgtcgcggc cggcggccgg gtgttggacg tcatggccgt gggtgccgac
     3361 ctggcatcgg cgagggacac ggcataccgc gcggtgtcct gccttgagtt ggccggtggg
     3421 caccatcgca gcgacatcgc gcttgctgcg gcgcgaggcg agatccacat cccagggagt
     3481 gacacatgag cgactcgacg gccctcgtcc tgccgggcta tgagcacatc tattcgggga
     3541 aggtccgcga cctgtatgcg ccgctcgatc cggtgacggg tgagcgccgg accgaccagg
     3601 tgctgctcgt cgcgtccgac cggatctcgg cgttcgacta cgtcctggac acccccattc
     3661 ccgacaaggg gcgggtgctg acgcagttgt cgctgtggtg gttcgaccgg ctcgtgggcc
     3721 tggagaacca tgtggtctcg accgacgtgc ccgccgcggt ggccggccgc gccgttctgg
     3781 tgcggcgcct ggagatgatc cccgtcgagt gcatcgcccg ggcctatctc accggcggcg
     3841 gcctgagcga gtatcggcag tccggggcgg tgtctggcgt cccgcttccg gccgggctcg
     3901 aggacggctc gcggctgccg gtgccgatct tcacgcccag caccaaggcc gcgataggcc
     3961 agcacgacga gccgatgacc atcgaggagg ttcagagcgc ggtcgggacc caggtggcgg
     4021 cccgcatcga cgtcttgacc cgccgaatcc tggcccgtgg caacgagatc gccggcgcca
     4081 acggcatcat catcgcggac accaaagtgg agttcggcat cgaccgctcc cgcgaggacg
     4141 acagcggcgg ccccgtcatc gtgctggccg acgaggtcct cacccccgac tcgtcacgct
     4201 tctggccggc cgatcagtgg cagccgggcc ggtcgcagcc gtcgttcgac aagcagttcg
     4261 tccgcgactg gctgacctcg ccggagagcg gttgggaccg ctcgtcgggt gaggctccgc
     4321 ccccgttgcc cgaacacgtc gtcgaggcga cccgggcgcg ctatctcgag gcctacgagc
     4381 ggatcaccgg gaacacgctt ccctgacggg tctgccggct ctgaccgacg agtctgccgg
     4441 gtctgacggg tatgccgcgt ggtcaggcgt cgtaggtggt gatgccctca acctcgggca
     4501 cggcgtgcca ctggccgtcc gcggccgact cgactgcggc atcggcggcg gccgcagccg
     4561 accagccatc ggcagccgac ggtgccaact gctgaccggt gagcaccgag cgcaggaaga
     4621 gctgggcctc gaccaccttg aggtcgtcga atcccatcgg gacgccggga ccgggctgga
     4681 agcgggcgaa gtcgccgtgc tgcggcccgg ccagcgacgt cgtgtagccg tgctcaggcc
     4741 cgttgccggt ggcgctgacc aggcaggtct gcagttcgtt gatcttctcg aagttccagc
     4801 gcagcgatcc gcccgtgccg tagacctcga cggcatactc cgagcgcggg ccgatcgaca
     4861 cgcgtgagga ttccagcgtg ccgacgacgc cgttgtcgaa gcgagcgagg atgccgacgt
     4921 agtcctcgtt gccgacgggt ccgcgctcgt cgccgatctc gaagccgccg tgcccgacgc
     4981 ccaccttggt ggggatcgga cgctcggtga tgaacgtcga gctcagcgcg gtgaccgagg
     5041 cgacccggcc gacgaggaac tgggcgaggt ccagcccgtg cgacatgagg tcaccgacga
     5101 ccccggagcc ggctcggtcc cggtcgtagc gccaggtgag gggcccttcg ggtgccgacg
     5161 catagtcggc gatcagccag acccggatgt tggtgatccg gccgagggca ccgtcgcgga
     5221 tgagctgccg ggcgcgggcg accgccggcg cgtgccggta gttgaacccg accgaggtga
     5281 ccactccggc cgcggcggcg gcgcgggcga tgtcggcgct ctcgtcggcg ctgacgccca
     5341 tcggcttctc gatccagaac ggcttgccgg ccgcggccgc cgcgagggcg atctccttgt
     5401 gcaggaagtt cggcgagcag atcgacacga catcgacctc ggggtcggcg agcagctcgc
     5461 ggtaatccgc gctggcgcgg gcgaaaccga gctgctcggc ggcatactcc cgcgcctccg
     5521 gcaccgggtc ggcagcgtgg acgaggcgcg tccgcactcc gaggtcggag taacgcagcg
     5581 gcgcggcgag gtaggacgtc gagtgcaacc ggcccatcca gccgaggctg atgacgccga
     5641 ctccgatgtc acgcatgagg attcgttccc ttgtgtctag gcggggcagc cggtcgggtc
     5701 acaggtagtg acgctgggcg gcctgctcgc ggacgtactc ggtgtatgcc gtctgggtgc
     5761 tctgcaggtc gctcacctgc gagaccggca cgtcccacca cgctgagccg ggcgggttgg
     5821 ggccatagag gtcggtctcg atatagagca ccgtcgtgtg gtcggaggcg gccgccgcgg
     5881 cgtagttggc ccggaagtcg gcaatggtcg cacatcgcag cacgtcggca ccccagctcg
     5941 cggcgttggc cgcgaggtcg aaggggacat gctcgccgtc caagcgtcca gaggtccggt
     6001 tgcgcatccg gtagttggtg ccgaagcgct gcgagcctcg cgactctgac agcccgccga
     6061 tcgacgcgaa gccgtggttc tgcagcagga cgatgatgac cttgacgccc tcggagacga
     6121 tcgtcgcgat ctcctgcgga agcatctggt aggtgccgtc accgacgatc gcgatgacct
     6181 cgtgcccgtc accgagggcc aacttggcgc ccagggcggc cgggatctcg tatcccatgc
     6241 aggagtaggc gtattcgacg tggtactgca cgggggtcga tgcgcgccac aggcattgca
     6301 ggtcaccggg catcgagccg gcggcgttga tgacgacgtc ctcgggcccc atcaactcgt
     6361 tgagggctcc gaagacctca gtctgggcgg gcagcggggt ctggtcgcgg tggtagcact
     6421 cctccacggc ccgctgccac gcggcatcga ggtcgacggc ttcctgccgg tatgccgcgt
     6481 cgacgctcca cccgtccagt gccccggtga gcgccgtgag ggcttcgcgg gcgtcggcga
     6541 cgagcatgct cgccgagtgc ttggctgcgt cgaagccgag ggtgttgatg ttgacgaacc
     6601 gcacgtcggg gttggcgaag atcgtgtggc tggcggtcgt gaagtcggag taccgagtgc
     6661 cgatgccgat gaccacatct gcctgtcgcg caagcgcatt cgcggccgag gtgccggtcg
     6721 agccgatccc gccgacggcg ctcgggtggt cccagttgat ggcgcccttg cccgcatggg
     6781 tgtcggccac ggggatgccg gtcgcggtgg cgaacgcgcg caactgctcg gacccatcgg
     6841 catacacgac accgccgccg gcgaccacga gcggccgctt tgccgatctg atgacctcga
     6901 ccgcgcgggc gagcgctgcg gcctcagggg ccgggcggcc gatgtgccag acccgcgggc
     6961 ggaacagtgc cacgggccag tcgaacgcct cggcctgcac gtcctgcggg aggcagatcg
     7021 tcacggcccc ggtgtcggcc gggtcagtga gcacccgcat cgcgcccagc aacgacggga
     7081 tgagttgctc gggccggttg atccggtcga agaagcgcga caccgggcgg aagacatcgt
     7141 tgaccgacac gtcgagggtt ccggggtcct ccaactgctg gagcaccggg tcgggatagc
     7201 gcgtcgcgaa ctggtcggag ggcagcagga gcaccgggat ccggttggtc gtcgcgagcg
     7261 cagcgccggt gaccatgttg gtcgcaccgg ggccgatcga cgtggtgatg gccatcgtct
     7321 gcaggcggtt cttggtgcgg gagtaggcgg ttgccgcgtg gacggcgccc tgctcgttgc
     7381 ggaccaggaa gtagggcagg tgctcgccgg gctcgcccgc gtcggcggcg gtctggtcct
     7441 gcagaagcgc ctgcccgacg ccggccacgt tgccgtgccc gaagatgccg aacatgcctg
     7501 tgacgagccg ctgttggacc ccgtcacgct cggagtgctg gttggccagg aaccgcacca
     7561 gggcctgggc ccccgtcagc cgcaccgtct cggtcatggt cgtcgtcctt ctcacgtggt
     7621 cggggtggtg ctcattcgga gggtatgccg cgggctcgcc tcacggggcg gggaggatga
     7681 cctcgcggac caggtggtcg agttcttcgt cagcggcgag gaactggcgc agcgtcttgc
     7741 gggtcgcgcc gaactcgtcg aactcctccg tcgtcatgcc gtcgggctcg taggcgcgac
     7801 ggaactccgg gatctgcagg agggcgtcga gaatggtcgg gtccaccggg atgtccatgc
     7861 ggggcgccgc gtccgcgccg ctggcctgga tcttctgctg ccagccgaac ggcggggaga
     7921 tgacgacgtc tccgccgaca aactcgctcc actgcaactg gttacggaag gccgccgaga
     7981 gcagccggga ccggaagcca cgctcctgga agatcgcgta ggcgtgcttg aaggccgcga
     8041 ccccggccca ctcgaggtag ctggggtcga tgtcgatccc gtcccgcttg acgacgtcct
     8101 tgagccagtc gtccaggcgc ccgaccatga tcgtcacgac ggggcccatg tgcgacacgt
     8161 cgtggcccgc cgcctcccgg gcagccaacc cgcgctcgat cgcctcggcc gccgcgacgg
     8221 cctgtgggac ggtgaaggac acggtgacgt tcatgctgac gccgcgtgcc gtggcatcct
     8281 cgatggcggc aattcccttg tgggtcgcgg gaatcttgac aatgacgttg ggcgccaggg
     8341 tggagaaccg ctcggcttgg tcggccagag caaccgcgtc gcggtgcagc cgcgggtcgg
     8401 tctgcatcga gagccgaccg ttgtggccct tgaacttctc gaaggccggt tcgagcagcc
     8461 tggcggcctc gatggagatt tcttcgacga cctgccaccc gatctgcgac tcagacgcgg
     8521 tcgggcgctg ttcggcgatc tccagcatcc gggccgacca gcggggcagg tcggccttca
     8581 cgcaggtgac cgcgatgacc gggttacacg tggccccgac ggccccccac tcgatggact
     8641 gggcgagctc gcgtgggtcg gccgagtcgt tccagagcgc ggtggtcgtc gtctgggtca
     8701 tcgtctgcag tggcgatgcg ttgtctgaca tgggttcctc cggggcgggc ggcgctggtg
     8761 gtccgagaca gcggtgtcgc ggcgaactcg gtgctggtga agctcgcggc tggtgagtcg
     8821 agactagctc tcgccctgcc tgccgtgtca atagcttgtc ctaacattct cgcaaattgt
     8881 tagcgcgagg atggaccgct gtgcggtgcg agcaggtcag ccggcgtggt gcttggcgac
     8941 gtagctctgc atcgtcgcga gctgaaagcg cgccatgtcg ccggccgtct cgtcctcggc
     9001 aaagacgctg ttgcacatga ctgttcccgg tcggtcgtag aagccgatct cgcccaaccc
     9061 gccgaagaag tcgtcccagt cgaggtcgcc gtcgccgatc ttgaggtgct ggtggacccg
     9121 ggcggtgttg ccgggcgggt tggagatgta acgcaaccca tgcgaacggt gatggtccat
     9181 cgtgtcggcg acgtggacga gccggatgag atcgccggcc tcgcgcatga tctcccgcat
     9241 cgggtgaccc atgtggtagc tgtggcaggc gacgaagacc atgccgacgt tggggctgtt
     9301 gagcccccgg atcacgcgga tggcctcgag gccgtcctcg acgaagtcgt cggggtgcgg
     9361 gtcgatgagc acgtcgatgc cctcgcgctc gatgatcggg agcaactcct ccatcgactt
     9421 gtagaaggct gcctcggact cctcggagcg ctctgggcgt ccggagaact ccgtgccgat
     9481 cacgctcacg ccgaggtcga cggtgatctg gacgatccgc ttccagttgc gcacggccgc
     9541 ctcccgggcg acctcgtccg ggccggacca gcgctgcacg ggaagcaccg aggcgatgcc
     9601 cacgccggcg tcgtcgcagg ccttcttgaa ggcgcgcacc agggcgtcgt cggccttcgg
     9661 gtggcggaag aacgggatga ggtcgcggtg cggggtcagt tggatgtgct cgtagccgag
     9721 gtcggccacc acccgcggga agtcgagcag gcggtagtcg tggtggaacg gagtcggatc
     9781 ctgggcgatc ttcatggtgc ctcctctggc gagcctgacg tcactatgtc aggacattag
     9841 cgccagtgcg cccgaagcgc catccccact ttgaactagc gctcgtgact cagcgctcgt
     9901 gaacggtgac atcaaacgcg tactgatcga accggtagca gtggtcacca aattcgatcg
     9961 ccgtgccggc cgcgtcgtag gcgcgacggg tcatcgtcag cagtgggtcg cccggcgcca
    10021 ggtccagccg cttgcgttcg gcgcttcgcg gacggcgggc cccgatcgtc tggtgggcga
    10081 cgacggggcg gaccccgcga gcccgcaaca cggcatacag actgccggag gcgagctcct
    10141 cctcggtgat gtcggcgaac tgcggcggga gccagttgtg catgagcgcc gtggggatgt
    10201 cgcccgccag gcgcaaccgc tcgacataga acagcggcgt gtcctgcggc aggttcaaag
    10261 ccgccgcagc ccggggggtg accgagtggc ggtcgatgtc cagcactcgc gtgatgggac
    10321 gcttgccctt gcgctccatg tcttcgaaca ggctcgtgag ctcgtcgcgg cggtggatga
    10381 cctcgctggc cacggccgtc ccgacgccgc ggcgccgcac caacaacccg cgcgacacca
    10441 actcggcgat cgcccggcga accgtcggcc gagagagatc cagccgatcg gcgagggcga
    10501 gctcgttctc gaacggatcg cccggctgca acgtgccgtc ctcgacggca gcggtgagct
    10561 gctcggcgag ctggtgatag agcgggacgg ggctcgcccg gtcgatcatc acggggatct
    10621 ggtgcacgaa accgagcata tgtcaggcac agtgttttgt cgtggatcag caattgtcct
    10681 aacaaacatt gacaacgcag gggtcggtcc ggcaccctgg gaaagccagc gcagcgacgc
    10741 acccagcgac aacgacagcc caggaggtgg acccgatgat ggccggcccg tccttcgacg
    10801 tgttgtgcat cggaagggtc ggtgtcgacc tttaccccat ggaggtcgga gtcggtctgg
    10861 aagacgtccg caccttcggg aagttcctcg gtggcagtgc cggcaacgtc gctgtggcgg
    10921 ccgctcgcta cggtcactcg gcagcgatca tcagccgcac cggcgacgac cccttcggtc
    10981 ggttcgtcgc gacggccctg ggcgacctgg gtgtcaacac cgactacctc gggatcgacc
    11041 cgtggctgcc caccccggtg accttctgcg agatcttccc gccggaccat ttcccgctct
    11101 acttctaccg atacccgcag gcccctgacc ttcgcgtcgc ccccgaacag atcccgccgg
    11161 tggcggtggc cgacagtcgg gtcttctgga cgacgctcac cggcctctcg gcagagccaa
    11221 gccggacggc ccacgcgacc gcgtggcacc tgcgcggacg cacggcgcac accgtcctcg
    11281 acctcgacta ccggccgatg ttctgggtcc acgagtctga tgcgagtcgt gcggctagcg
    11341 acgcgcttga gcaatcgacg gtggccatcg gcaacctcga ggaatgccga gtggcggtcg
    11401 gagagaccga ccccgaccgc gcggcccgcg ccctgctcga ccgcggggtc gatctggcga
    11461 tcgtcaagca aggacccaag ggcgttctcg cgatgaccgc cgacgagcgg gttgaggcac
    11521 ctccggtctg ggtggaggtc gtcaacgggc tcggagcagg tgacgcgttc ggcggtgccg
    11581 tctgccacgg cctgctcaac ggctgggacc tggccgagac catctccttc gcgagcgccg
    11641 cgggtgccca tgtcgcgggc cggctcgagt gtgcgacagc gatgcccgac gaggccgagg
    11701 tccgggcact gcaggcccga gcgggtcggt cgtgacgccg cccctcgacc tggcctcggt
    11761 gatgcgccgg cggatgctgg agccggacgg catacctgcg gcgtgggcgg cgcggcgcgc
    11821 cggtgccgtg cccgccgatg gtcggttgct cgtcatcgcc gccgaccatc cggcgcgtgg
    11881 agccctgggc gtccgtgacg accggcgcgc catggcatcc cgctcagatc tgttgcagcg
    11941 cttggcgatt gccgtctcca ggccgggcgt cgacggagtg ctcggcaccc ccgacatcct
    12001 cgaagacctg ctgctcgcgg ggctgctcga cggcctcctc gtctacgggt cgatgaaccg
    12061 agcgggcctg cagggggcgg tgttcgagct cgacgaccgg ttcacggcat acgacgcggc
    12121 gaccattgag cggctgggtc tggacggggg caagatgctc gcccgcgtcg cctacgacga
    12181 ccccgcaacg gcgccgacgc tggaggcctg cggccgggcg gtcaccgatc tcgccgagcg
    12241 aggcaagatc gccctcgtcg agcccttcct ctccagccgc agcgccgacg gttgggtgcg
    12301 caacctcctg gaccccgact ccgtgatcac ctccgtcgcg atcgcctccg gcctcggcgc
    12361 aacgagcgcc cacacctggc tcaaactgcc ggtcgtgccc gagatggagc gggtcctcga
    12421 ctcgacgacc ctgccgacac tcctgctcgg cggcgacccg accaacgagc ccgcgcagac
    12481 ctacgcgacc tggggtcggg ccctggcctg tccgcccgcg gtgggcctcg tcgtcggccg
    12541 cgccctgctc tatccaccgg ggggcgacgt cgcggcagcg gtcgatgtcg ccgccgatct
    12601 cgtccacgga ggcgctcgat gagtgattgg ttgcggcccg ccggctccag cggcgacgcc
    12661 gagttcgacg tccgcatcgc ccccggcgac cccgggtggt cgcacaccgg cctgtatgcc
    12721 gtgaccctcg ccgccggcga gtcacgcacc gtcgacaccg gtgactgcga gtggatcgtc
    12781 ctgcccctct ccggtggctc ggcggtcgtg gaggtcggcg agcgggtcta cttcctcacc
    12841 ggccgggccc acgtcttcgc cggcccgacc gacctggtct acgtcccgcg cgattcgcgg
    12901 ttcaccgtga cgagcacgga cgggggccgg atcgccttcc ccttcgccaa ggccagccgc
    12961 gccttcccgg tatgccgcgt ggacagttcc caggtccccg tcgagatgcg ggggaccggg
    13021 agttcctccc gtcaggttca gaacttcggg actcccggtg tgctggaggc tgattcgatc
    13081 attgcctgcg aggtcatcac cccggctggc aactggtcgt cctatccgcc ccacaagcac
    13141 gacgaggacc gtgacggcgt cgagtcggcg cttgaggaga tctactacgt cgaggttcag
    13201 cgcgagggtg gtgacgggag cgcggcgggg atcgcctatc tgcgcaacta cggcaccgag
    13261 tccgcgccga tcgacaccct ggccgaggtg cgctcgggcg acgtcgtcct cattccgcac
    13321 ggctggcatg gcccggcgat ggcgccgccc ggctacgacc tctactacct caacgtcatg
    13381 gccggccccg gcgagacgcg ggcctggctc atctgcgacg accccgcaca cgcctgggtc
    13441 cggggcgcgt gggtcgacgc accggtggac gcgcggctgc ccttcacgca ggtgccctga
    13501 cgccgtgcgc atcggaatcg ttggcgtggg ccggatcggc tcgttccacg cacagaccct
    13561 cgcagccgac ccgaccgtca ccgacctcgt cctgctcgac ccgtccgcga cggcggctgc
    13621 cgcggtcgcg tcccaactcg gtgcgacggt cgcccctgac ctcgacgccc tcctgcggcg
    13681 agacctcgac gccctgctcg tcacctcggc gacccacacc catgccgagg tcctcctcgc
    13741 cggcgtgcgg gcgggcctcc cgaccttctg cgagaagcct gtcgcggcca gtctcgacga
    13801 gaccatcgag ctcgcgcggc tcgaggccgc gtccggagtc ccgatccagg tgggcttcca
    13861 gcggcggttc gacacggcat acgggcgcct gcgtgccgcg atcgcctccg gtgagttggg
    13921 cttcgtccac acgatccgcg ccgcgacgca cgaccagagc ccgccgcacc ccggctacct
    13981 cccgacgtcc ggtgggttgt tccgcgacgc ggcgatccac gactacgacg tcatccgctt
    14041 cgtcacggga cgcgaggtcg cgcaggtgta tgccgcgggc gccaacaagg ggcagaagtt
    14101 cttcaccgag gcgggagacg tcgacaccgc agccgcgctg ctcacgctcg acgacgacac
    14161 cctcgtcgtc acgtcggtga ctcgctacaa cagggccggg cacgacatcc ggatggaggt
    14221 ccacggctcg ctgggcgcgc tcgccgcagg cctggacgac cgctacccga tgacctcggc
    14281 cgagccgggt gcgaggttcc ccgcgggtcc cgcgcaccag tccttcctcg aacggttcgc
    14341 cgcggcctat cgtgcggaga tgactgagtt cgtccaggtc gcagccggtg cggtgccgag
    14401 ccgatgcacg gtggccgacg cgctggaggc gttccgggtc gccgaggcct gtgggatctc
    14461 ccgccgcgag ggccgcccgg tggcgctggc cgagatcccc ggtgcgtgag tcgtccggcg
    14521 tcgggctcct gatccgtccc gtcccatccg agtctggtgc ccagcctcgt cttacgttaa
    14581 tatgttagaa caaatgagac cacgacccaa ggagcgcgca tgagcacccc ccgccgcatc
    14641 acccatctca tcgggcattc cgaatgggac gggaccgccg agcggaccgg tgatgtcttc
    14701 aacccggcga ccggcgaggt gaccggcaag gtcgacttcg cgtcgaaggc actggttgac
    14761 gaggtcgttg ctaacgctgc cgtcgccgct gccgagtggg gtcgggcgtc gctggccaag
    14821 cggacccagg tgctgttcgc cttccgccag ctgctcaacg acaacaagga gcgcgtcggg
    14881 gcgctcatca ccgccgagca tggcaaggtg acctccgacg cgatcggcga ggtgacccgt
    14941 ggtctggaga tcgccgagtt cgcctgcggc atcccgcacc tgctcaaggg tggcttctcc
    15001 gagaacgtct cgaccaacgt cgacgcctat tcgatcctgc agccgctcgg ggtcgtcggc
    15061 gtgatctcgc cgttcaactt cccggccatg gtgccgatgt ggttcgtgcc catcgccatc
    15121 gcctgcggca acgccgtcgt catcaagccg tccgagaagg acccgtcggc ggtcaacgcg
    15181 gtggcggcgc tgtggcgcga ggctggcctg cccgacggcg tcatgagcgt cgtccatggc
    15241 gacaaggagg cggtcgacgc actcctggac aacaccaccg tcaaggcgat ctcgttcgtc
    15301 ggctccacgc ccatcgcgcg ctatgtctac gagaagggca cggccaacgg caagcgggtc
    15361 caggccctcg gcggcgccaa gaaccacatg ctcgtgctgc ccgacgccga cctcgacctc
    15421 gcggccgacg ccg
//
LOCUS       DAOMZY010000032        15365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_3477_length_15365_cov_5.962639, whole genome shotgun sequence.
ACCESSION   DAOMZY010000032 DAOMZY010000000
VERSION     DAOMZY010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 15365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15365
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3477_length_15365_cov_5.962639"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PLK64_03195"
     CDS             <1..425
                     /locus_tag="PLK64_03195"
                     /inference="COORDINATES: protein motif:HMM:NF023691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 4"
                     /protein_id="HPV78776.1"
                     /translation="DGMKAMWRIFLILMIFFFGTVPFYAAWQQKDYGAVLEIAGPIAL
                     FITALMMALAGWYLWVTSRKFENLPDDNPDGEISDIEGDYGFFTPSSWWPLALGFSSA
                     LAFAGLAVGWWLFMIAFVIGCVSLVGWTFEHFKGDYAN"
     gene            complement(497..859)
                     /locus_tag="PLK64_03200"
     CDS             complement(497..859)
                     /locus_tag="PLK64_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HPV78777.1"
                     /translation="MAHRRNRLTPEQFDAAVEEALEFIPAQFRQAMANVAVLVEAEPP
                     EGEEELLGVYDGVPVTDWGQDWGGSLPDRVTVFQGPLERMCATREELLDEIAVTVVHE
                     MAHHFGIDEERLHELGWG"
     gene            1004..1282
                     /locus_tag="PLK64_03205"
     CDS             1004..1282
                     /locus_tag="PLK64_03205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HPV78778.1"
                     /translation="MRLSIRVRPGASRTRVGGCYGAGEDAVLTVAVSARAVDGAATAA
                     VMDAVAAAFGVRRRDVTLVAGATSRTKVLDVDVEEEAGRARLAHLLTV"
     gene            complement(1418..3139)
                     /locus_tag="PLK64_03210"
     CDS             complement(1418..3139)
                     /locus_tag="PLK64_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HPV78779.1"
                     /translation="MSTTASARTAADLRAADAKGRTGSESKAGAAGVWVDERTGLAKG
                     AAYMMKKVFPDHWSFMLGEVAMYSMIICLLTGVFLSFWFVPSAGQIPYNGSYLPLQGV
                     IMSEAYNSTLAISFDIRGGLLIRQIHHWAALMFCVAITVHMLRVFLTGAFRKPRELNW
                     VIGSILSMLAIVEGFAGYSLPDDLLSGTGIRAAEGFIRSIPLVGSYISYLLFGGTFPG
                     EAIIPRLYSVHVLLLPAVLIGLFTAHIVLVMVHKHTQFPGPGRTNENVVGFPLMPVYT
                     AKAGGFFFMVFGVLAIISALVTINPIWAYGPYDPSPVTAGSQPDWYMGFADGALRLLP
                     GFLEFELFGFTWSMNVFLGAVALIPLMYTAAGVYPWIEAWVTGDKREHHLLDRPRNAP
                     TRTAIGMAAFSFYVVLMFAAGNDIMAIRLQLSINDVTILFRMLVFVVPVIVFWVTRRI
                     CWSLQRHDREKVLHGRETGTIIRTAEGKFFERHEDIDAFERWPLVAFESPAPLALTDT
                     ADANGVSSKDAKKARRRVVWSNFYFKTRVEPVTPTELAAAHHHGEHEAISGGSAPAAI
                     KHDKHPH"
     gene            complement(3136..4278)
                     /locus_tag="PLK64_03215"
     CDS             complement(3136..4278)
                     /locus_tag="PLK64_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HPV78780.1"
                     /translation="MSDQVTPTSGSASGAGTVSLDEGHHVGTGGLPATFENPGLPPHV
                     HRAADDDPKAAKRAERQVAVLFTLSILGTLGFIVSYFAIDRMTSVFLPFIGLTNLLNV
                     MLGVCLGIALLGIGFGAVHWAKTLMSDEEVVEERHAIASSDAARADFVGALAAGAESA
                     QLARRPLIKATAGAALGLFALPLGVQLAGSMGNPTKALEELKQTIWDVPKDGKPIRLV
                     RDPELTPIKASDVTIGSVFHVMPETLKELREHNVKEGHSEWHGVLEAKAKAAVLLMRL
                     NPSEFKDDVLGRKARDWGFQGIVAYSKICTHVGCPVGLYEQQTHHLLCPCHQSTFDVT
                     RDCEVIFGPAKRPLPQLKITVDDEGYLVAPQGFAEPVGPSYWERLK"
     gene            complement(4316..5101)
                     /locus_tag="PLK64_03220"
     CDS             complement(4316..5101)
                     /locus_tag="PLK64_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HPV78781.1"
                     /translation="MNALAARRRHPAAIAILLFLGLALVGTAYSLLAPKPAQAAVATA
                     DDAELGKQLFLANCATCHGLNAEGTKSGPTLMGVGAASVDFQVGTGRMPLAGPNVQAP
                     RIDEVKFTEEQIAAMAAYVASLAPGPAIPDAKLTSGEGGDVALGGEIYRVNCAMCHNF
                     AGSGGALTRGKYAPSLYGVDGRYVYEAMITGPQSMPVFNDTNLNPEAKASVIAYLKST
                     QAEPNVGGMTLGNLGPVSEGMFVWIFGLLLMIACAFWLGQKAA"
     gene            complement(5148..5804)
                     /locus_tag="PLK64_03225"
     CDS             complement(5148..5804)
                     /locus_tag="PLK64_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HPV78782.1"
                     /translation="MARVATASAIPAHPVSSGHGPATRPNMVQVGTMVWLSSELMFFA
                     GLFAIYFQLRSVSAELWADQTAKLNVTFATINTAILVISSVWCQIGVMKAERGQASRT
                     GKLTEIGKWGMREWYVVTYLFGATFIAGQVYEYAKLVSENVTLSSDRYGSVFYMATGF
                     HGLHVTGGLIAFLLIIGRTFTTRRYTHTQAVGAVVTSYYWHFVDVVWIALFAVIYLLK
                     "
     gene            5989..6441
                     /locus_tag="PLK64_03230"
     CDS             5989..6441
                     /locus_tag="PLK64_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78783.1"
                     /translation="MTASQAHSASPAVPASVHQISVLLYSDDVTVRDAVRLAVGRRPA
                     KNVEVTSWLECATHAAVVEAVDNRRFDVLILDGEATPMGGMGLCRQLKNEIFQCPPTL
                     VLTGRVQDGWLAAWSQADAVVPHPLDPVALARAVTQLAGAGAGATAAH"
     gene            6503..7513
                     /gene="trpD"
                     /locus_tag="PLK64_03235"
     CDS             6503..7513
                     /gene="trpD"
                     /locus_tag="PLK64_03235"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HPV78784.1"
                     /translation="MLQRGHLSRQDCAWAMGEIMSGGATPSQIAGFLMALHAKGETVE
                     EMRGLADEMLAHATRIQAPELSLDVVGTGGDRAQTVNISTMAAMVCAAAGVSVVKHGN
                     RAASSRSGSADVLEALGIDLTLPAARVEALAHEVGITFCFAQQFHPSMRHVAGTRREL
                     GVPTAFNFLGPLTNPGQPTYAAVGVADARMAPIIAGVFAGRGRDAAVFRGDDGLDELT
                     LATTSTVWWVRSGDVVEHQLDPIRLGLTLSPTEALRGGDAAYNAAVVRDVFGGARGPV
                     RDAVVLNAGAALAVAAANDSGRTPAAVHADVRAGMDRAEAALDSGAADALVQRWVEAT
                     RA"
     gene            complement(7753..8031)
                     /locus_tag="PLK64_03240"
     CDS             complement(7753..8031)
                     /locus_tag="PLK64_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HPV78785.1"
                     /translation="MITAIVLVTCDAARIPEVAQDIAALDGVTEVYSVTGDADLVAMV
                     RVREHEQLADVIADRLSKVEGVAATSTHIAFRTYSRHDLEAAFSLGLD"
     gene            complement(8066..9763)
                     /locus_tag="PLK64_03245"
     CDS             complement(8066..9763)
                     /locus_tag="PLK64_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HPV78786.1"
                     /translation="MHAVQGTLDDLGTPLHAVTFVVVDLETTGGSANECAITEVGAVK
                     VRGGEVLGEFQTLVNPGSPIPAFIQVLTGISDGMVAQAPRIDTVLPAFLEFARGSVLV
                     AHNAGFDITFLKAAAEATGQSWPGFKVVDTVTLARQLVSKDEAPNRRLGSLAALFGAD
                     TTPDHRALHDARATVDVLHALLARVGNVGVQTLEELASYSSRVTTAQRRKRFLADDLP
                     TAPGVYVFKDAKGRPLYVGTSRNIRVRARSYFTASEQRTRMAEMVSVAASIQPIVCQT
                     TLEAQVRELRLIAEHKPRYNRRSTRPERALWVKLTAEPYPRLSIVREVRDDLASGARY
                     AGPFGSRAAAEAAVAAVHDVLPLRQCTTRLSTKGTGSACVLAEMGRCGAPCEGRQSLS
                     EYAVHVETAAALLVGDGRSMVDALCERMTVLAKAERFEDAGALRDRLGHLVRGLARAQ
                     RLAPLAASPELVAARRTDAGAWELVCVRHGRLAGTSLAPRGADPLVYVESLRATAEAV
                     PPPAGPAPAAYPEETELVLRWLESPGVRIVSLDGEWTCPVGGAGAAKATFDDLRRGA"
     gene            9906..10142
                     /locus_tag="PLK64_03250"
     CDS             9906..10142
                     /locus_tag="PLK64_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78787.1"
                     /translation="MIVTCATCPVRGLRCDDCVVTALATISVGPPGERPLDAKERRAV
                     GLFVSAGLLDSGYAATLTSTVDSGRVGRVGRAVG"
     gene            10193..11668
                     /locus_tag="PLK64_03255"
     CDS             10193..11668
                     /locus_tag="PLK64_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78788.1"
                     /translation="MTAFGRRSAWGRWLPAADVDGRRASLVTAIVTAHGAAVVTALVA
                     GVVVALVMSGCTPNAATPSTTATASTRANSGANAGADAVAGAGSEASEAARTALTTLM
                     AARSRALETGDRTAWLATIADPHGPDGSAEEAAYAGLVALGVREVIVSEVRRSATQAT
                     SAAPAAEESQHAQTLTTQTWTGSMRLGYAIPGFDRGVRWVARTVTLTQVAGQWLIQRW
                     VGPADRWEVFDLSPLQVARSERALVAGPVAQDVLRDRLAEVEVGQDRVAAALGRAFPA
                     VVVVPATTDQAALLLGNGAPSSEQSPLAGQVAATTHGPREPSATAVADRVVLDPQGMG
                     RLTPAGRRVVLTHELTHVSVRAGTLHDLPLWLSEGFAEWVAFRDEGMDVTVVAARLLS
                     QVRASGPPATLPTPTDFAGTAGDPAAAYQESWLAASRIATLAGPDGLVGLVRRVGGVP
                     GASSSPAGPVDPAAALTEVLGQSMSQFLAGWQSELVTLAAG"
     gene            11732..12175
                     /locus_tag="PLK64_03260"
     CDS             11732..12175
                     /locus_tag="PLK64_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPV78789.1"
                     /translation="MAERTESSIHVDAKPGVVLDVIADFEAYPEWAAEVKRVSVLSEE
                     GDGWADRVEYTLDAGVLKDTYTLDYEWDISDDGTGVVSWSLVRATVLKAMNGSYTIAR
                     DKAGSKVTYRLAVDLNIPMLGMLKRKAEQQIVDAALKELKKRVEG"
     gene            12178..12558
                     /locus_tag="PLK64_03265"
     CDS             12178..12558
                     /locus_tag="PLK64_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78790.1"
                     /translation="MSVEGSDRVGSVAEEAARLLGVFAAGMPEAHLLAAGSRAAGETP
                     TAHACPECGHDPHAASSGDSVCRICPICRVLNVVKAISPETLDKIADVVDLVSEGLRG
                     FAATRRTEAPPPAPTGGEQPQEPV"
     gene            12555..13664
                     /locus_tag="PLK64_03270"
     CDS             12555..13664
                     /locus_tag="PLK64_03270"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011691408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HPV78791.1"
                     /translation="MSAAYTIGIDIGGTKVAGGVVDVDGVILAKARADTPHRSTSPEV
                     VESTIVGVVEDLIAQVGPGQVSAVGIGAAGFVGADRATVVFAPHLSWRQEPLRDALFD
                     RIGLPITVDNDANASSWAEWRFGAGRGESHLVMVNLGTGIGGGIVLGGRVERGRFGIA
                     GEFGHMQVVPDGLRCECGNRGCWEQYSSGNALVREARTLLLANSPVADDLRARVGGDP
                     ALLTGPLVTEAAQAGDRTAIELLADVGRWLGVGIANLAAAFDPGVFVIGGGVSAAGEL
                     LLGPARETYRRQLTGRGYRPEAKIVAAQLGNDAGVIGAADLARAEAQPEVLAEASAPS
                     ADGGTREVVTSAESAEATSHPGARRVKWWRRRHTD"
     gene            complement(13699..14322)
                     /locus_tag="PLK64_03275"
     CDS             complement(13699..14322)
                     /locus_tag="PLK64_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78792.1"
                     /translation="MNDEDALEADDQGRSEPAGGPEETSATALPPLNEIDAAFASIVA
                     HWDDPTPEPLADRAADDAAGDAADLTAPAPLAPAPLPPAYPATAHVAAPVGDAPDATT
                     DHFSAAEDADEERLFGWRGHTPPPEDEHFQPPPPNLPPVHDATYWLAVAGMTLGPLLV
                     IWAAMVSRNPDPGWWVLTGIVLTVAGFGLMVLRGSGERDPDDNGAQV"
     gene            14448..>15365
                     /locus_tag="PLK64_03280"
     CDS             14448..>15365
                     /locus_tag="PLK64_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPV78793.1"
                     /translation="MFYWFLKTVVLGPILKLLFRPWVEGAEHIPERGAAILASNHLSF
                     SDSIFLPIVMSRRITFPAKMEYFTGSGVKGRLTAWFFKGVGQIPIDRSGGRASEAAIS
                     SGLKVLRRDELFGIYPEGTRSPDGRLYKGKTGIARMALEAGCPVIPVAMIGTDKAQPT
                     GQKLPKIMRIGVRIGAPLDFSRYEGMENDRFVLRSITDEIMYELMLLSGQEYVDVYAT
                     SLKKNVLKDTVQTAVAKAQEIQKTAQEIQKTAAEAAKAATEAARAVTESAKTRATHPQ
                     EPLETHEAVEAPDSATSTGSVDDDGPSSDE"
BASE COUNT         2403 a         5032 c         5565 g         2365 t
ORIGIN      
        1 gtgacggcat gaaggccatg tggcggatct tcttgatcct catgatcttc ttcttcggca
       61 ccgtgccgtt ctatgcggcg tggcagcaga aggactacgg agcagttctc gagattgccg
      121 ggcccattgc gctgttcatc acggcgctga tgatggcact ggccggttgg tacctatggg
      181 tgaccagccg caagttcgag aaccttccgg acgacaaccc ggacggcgag atctcggaca
      241 tcgagggtga ctacggcttc ttcaccccga gctcgtggtg gccgctggcg ctcggcttct
      301 cgtccgcgct ggccttcgcc ggcctggccg tcggctggtg gttgttcatg atcgccttcg
      361 tcatcggttg cgtctcgctc gtcggctgga ccttcgagca cttcaagggc gactacgcca
      421 actgatccat acggcatacc cgaagggccg gctccgcatc gcggaaccgg cccttcgcgt
      481 atgccgcctg ctctgctcag ccccagccga gctcgtgcag tcgctcctcg tcgatgccga
      541 agtggtgagc catctcgtgg acgacggtga ccgcgatctc gtcgagcaac tcctcccggg
      601 tcgcgcacat ccgctcgagg ggcccctgga agaccgtcac gcggtccgga aggctcccac
      661 cccagtcctg gccccagtca gtgaccggaa ccccgtcgta gacgccaagg agttcctcct
      721 caccctcggg aggttcggcc tccacgagga cggccacgtt ggccatcgcc tggcgaaact
      781 gggcagggat gaactcgagg gcctcctcga cggcggcatc gaactgctcc ggggtgagcc
      841 gattcctgcg gtgagccacg atggcgagcc tgccatagtg ggcgcatgag acccggttgc
      901 ctggctgtcg ccgacccgac gagcgcggtg aggtgacggt gcacggcata cagcagcgcc
      961 tggtggccct ccacgggtca ccgaggcccc agggggtccg cgagtgcgtc tgagcatccg
     1021 cgtgcgccca ggcgcgtcca ggacgcgcgt agggggctgt tacggcgccg gggaggacgc
     1081 cgtgctgacc gtggcggtct ctgcgcgcgc tgtggacggt gcggcgacgg cggccgtcat
     1141 ggatgccgtg gcagcggcgt tcggcgtgcg ccgtcgagat gtgacgttgg tggccggcgc
     1201 gaccagccgg accaaggtgc tcgacgtcga cgtcgaggag gaggcaggcc gcgccaggtt
     1261 ggcgcacctg ctgacggtct gacgggcgcc ctcggccgca tgagtggcta agtgagcggc
     1321 ttacatgagg cggcagggga gccggcgggg gagtgacctg gcacaaccga agggctcccg
     1381 gaccggagtc caggagccct tcagggtgac cgttgggtca gtgcgggtgc ttgtcgtgct
     1441 tgatcgccgc cggagccgat ccaccggaga tggcctcgtg ctcgccgtgg tggtgagcgg
     1501 ctgccaactc ggtcggggtg actggctcga cgcgggtctt gaagtagaag ttcgaccaca
     1561 cgacgcggcg acgtgccttc ttcgcatcct tggaggacac accgttggcg tcggccgtgt
     1621 ccgtcagcgc cagaggtgcg ggggactcga aagcaaccag cggccaccgc tcgaaggcgt
     1681 cgatgtcctc gtgccgctcg aagaacttgc cctcggcggt ccggatgatc gtgccggtct
     1741 cgcgaccgtg caggaccttc tcacggtcgt ggcgctgcag cgaccagcag atccgtcgcg
     1801 tcacccagaa gacgatgacc gggacgacga acacgagcat ccggaagagg atggtgacgt
     1861 cgttgatcga caactgcagg cggatggcca tgatgtcgtt gcccgcggcg aacatgagca
     1921 cgacgtagaa cgagaacgcg gccatgccga tcgcagtgcg ggtcggcgcg ttgcgcgggc
     1981 ggtcgagcag gtggtgctcg cgcttgtcgc cagtcaccca ggcctcgatc cacgggtaga
     2041 cacccgcggc cgtgtacatc aacgggatga gggccacggc gccgaggaag acgttcatgc
     2101 tccaggtgaa accgaacagc tcgaattcga ggaaacccgg caggaggcgc agggcgccgt
     2161 cggcgaagcc catgtaccag tcaggctgcg aaccggccgt gacgggggac gggtcgtaag
     2221 gaccgtaggc ccagatcggg ttgatcgtca ccagcgccga gatgatcgcc aggacgccga
     2281 acaccatgaa gaagaagcca ccagccttgg cggtgtagac cggcatcagc gggaagccga
     2341 cgacgttctc gttggtgcgg ccggggccgg ggaactgggt gtgcttgtgg accatgacca
     2401 ggacgatgtg cgcggtgaac aggccgatga gcacggccgg cagcagcagg acgtgcaccg
     2461 agtagagccg cggaatgatc gcctcgccgg ggaaggtgcc gccgaagagc aggtaggaga
     2521 tgtaggaccc caccagcggg atcgagcgga tgaagccttc ggcggcgcgg ataccggtgc
     2581 ccgagagcag gtcgtccggc agcgagtagc cggcgaagcc ttcgacgatg gccagcatcg
     2641 acaggatcga gccgatgacc cagttgagct cgcgcggctt gcggaacgcg ccggtcagga
     2701 acacgcgcag catgtgcacc gtgatggcga cacagaacat cagggcggcc cagtggtgga
     2761 tctgccggat cagcaggccg ccacggatgt cgaacgagat cgccagcgtc gagttgtacg
     2821 cctcggacat gatcacgccc tgcaacggca ggtaggagcc gttgtagggg atctggccgg
     2881 cgctcgggac gaaccagaac gacaagaaga caccggtcag caggcagatg atcatcgagt
     2941 acatcgccac ttcaccgagc atgaagctcc agtgatcggg gaagaccttc ttcatcatgt
     3001 atgccgcgcc cttggcgagg ccggtgcgct cgtcgaccca cacgcctgcg gcgccggcct
     3061 tgctctcgga gccggtgcgg cccttggcgt cggcggcgcg caggtctgca gcggtgcgcg
     3121 cggaggcagt ggtgctcact tgaggcgctc ccagtagctc gggccgacag gctcggcgaa
     3181 gccctgcggg gcgacgaggt agccctcgtc atccacggtg atcttcaact gcggcagcgg
     3241 acgcttggcc gggccgaaga tcacctcgca gtcacgggtg acgtcgaagg tcgactggtg
     3301 acaggggcag agcaggtggt gggtctgctg ctcgtagagg cccaccgggc acccgacgtg
     3361 ggtgcagatc ttggagtagg cgacgatgcc ctggaagccc cagtcgcggg ccttacggcc
     3421 caagacgtcg tccttgaact cggacggatt gaggcgcatg agcaggacgg cggccttggc
     3481 cttggcctcc agcacgccgt gccactcgga gtggccttcc ttgacgttgt gctcgcgcag
     3541 ctccttgagc gtctccggca tcacgtggaa gaccgagccg atcgtcacat cggaagcctt
     3601 gatgggggtg agctcggggt cgcggaccag ccggatcggc ttgccatcct tgggaacgtc
     3661 ccagatcgtc tgcttgagct cttccagagc cttcgtgggg ttacccatcg acccggcgag
     3721 ctggacaccc aagggcaggg cgaacaaccc gagggccgcg cctgcggtcg ccttgatgag
     3781 tgggcgacga gccaactggg cggactctgc gcccgcagcc agggcaccga cgaagtcagc
     3841 acgtgccgcg tcggaggagg cgatcgcgtg ccgctcctcg acgacctcct cgtcggacat
     3901 gagggtcttg gcccagtgga cggcgccgaa accgatgccc agcagggcga tgcccaggca
     3961 cacaccgagc atgacgttga gcaggttggt cagcccgatg aagggcagga agaccgaagt
     4021 catccggtca atggcgaagt agctgacgat gaaccccagc gtgccgagga tcgacagcgt
     4081 gaacagcacc gcgacctgac gctcggcgcg cttggccgcc ttggggtcgt cgtcagcggc
     4141 gcggtggacg tgcggtggca gcccggggtt ctcgaacgtc gccggcagac cgccggtgcc
     4201 cacgtggtgc ccctcatcga ggctgacggt gccggcgccg ctcgccgacc cgctggtggg
     4261 ggtgacctgg tcactcatga ggtcatcgat cctgtcgtcg tggggtaggc ggggctcacg
     4321 cggccttctg gccgagccag aaggcgcagg cgatcatgag caggagaccg aagatccaaa
     4381 cgaacatgcc ttcggacacc gggccgaggt tgcccaaggt catccctccg acgttcggct
     4441 cggcctgggt cgacttgagg taggcgatga ccgaggcctt ggcctccggg ttgaggttgg
     4501 tgtcgttgaa gaccggcatc gactgcgggc ccgtgatcat cgcctcgtag acgtacctgc
     4561 cgtcaacgcc gtagagcgac ggggcgtact tgccgcgggt gagcgcgccg ccggatccgg
     4621 cgaagttgtg gcacatcgcg cagttgaccc ggtagatctc gccgccgagg gcgacatcgc
     4681 cgccctcacc cgaggtcagt ttggcgtcgg ggatcgcggg gccgggagcc aacgaggcga
     4741 cgtaggccgc catggccgcg atctgctcct cggtgaactt gacttcgtcg atccgcggcg
     4801 cctggacgtt ggggcccgcg agcggcatac gccccgtgcc gacctggaag tccactgacg
     4861 cggcgccgac ccccatgagc gtcggaccgg acttggtgcc ttcggcgttg agaccgtggc
     4921 aggtcgcgca gttggcgagg aacaactgct tgccgagctc cgcgtcatcg gccgtcgcga
     4981 cggcggcctg ggcgggcttg ggggcaagca gcgagtatgc cgtcccgacc agggccagcc
     5041 cgaggaacag caggatggcg atggcggctg ggtggcggcg acgtgcggcc aaggcgttca
     5101 cgatgtcgtc ctgtcgtggg ttctcgcggg tcggggggtg tcgatgctca cttgagcagg
     5161 tagatcacgg cgaacagcgc gatccacacg acatcgacga agtgccagta gtaagaggtg
     5221 acgaccgcgc ccaccgcttg ggtgtgggtg taccggcggg tcgtgaaggt gcgcccgatg
     5281 atgagcagga acgcgatgag gccgccggtg acgtgcagac cgtggaagcc ggtcgccatg
     5341 tagaacacgg agccgtagcg gtcggaggag agcgtcacgt tctcggacac cagcttggcg
     5401 tactcataga cctggccggc gatgaaggtt gcgccgaaga gatacgtcac gacgtaccac
     5461 tcgcgcatgc cccacttgcc gatctcggtg agcttgccgg tgcgcgatgc ctgaccgcgt
     5521 tcggccttca tcacgccgat ctggcaccaa accgacgaga tgaccaggat cgccgtgttg
     5581 atggtcgcga aggtcacgtt gagcttcgcg gtctgatctg cccagagctc ggccgagacc
     5641 gaccgcagct ggaagtagat cgcgaagagc cccgcgaaga acatgagttc ggaggagagc
     5701 cagaccatgg tcccgacctg gaccatgttc ggtcgggtcg ccggaccatg cccgctgctc
     5761 actgggtgcg ctggaatcgc cgatgctgtc gccacgcgcg ccattatggc gtgccgagtg
     5821 tcggcatgtg cgaccgaccc cccggttaat cgccaaacga gtcaacttcg tgctcagcgg
     5881 ggcccatgag gttggtcacc acaggtgctt cccgccaacg gagaggtatg ccgccgcgcc
     5941 gcccccggcc cgcgcgggcg cgcgcacgtg gcgactagca tgagcgacat gaccgcatcg
     6001 caggcgcact cggccagccc ggccgtcccc gcatccgtcc accagatctc cgtattgctc
     6061 tatagcgacg acgtgacggt ccgcgatgcc gtgcgcctcg cggtcggccg acggcccgcc
     6121 aagaacgtcg aggtgacctc ctggctggag tgcgccaccc acgctgccgt cgtcgaggcg
     6181 gtcgacaacc ggcgtttcga cgtcctcatc ctcgacggcg aggccacccc gatgggtggg
     6241 atgggcctgt gccgtcagtt gaagaacgag atcttccagt gcccgccgac gttggtgctc
     6301 accggccgag tccaggacgg gtggctggcg gcctggtcgc aggctgacgc cgtcgtgccg
     6361 catcccctcg acccggttgc gctggctcgg gcggtgaccc agttggctgg ggccggggct
     6421 ggcgccacgg ccgcgcactg acctcgggtc gacggtgaac aacgaggtag ggacccagtc
     6481 ctggccagag gtctactcgc ggttgctcca gcgtggtcat ctctcacgtc aggactgcgc
     6541 ttgggccatg ggcgagatca tgagcggcgg ggcgaccccc tcccagatcg ccggcttcct
     6601 catggcgctg cacgccaagg gcgagaccgt ggaggagatg cgcggcctgg ccgacgagat
     6661 gctcgcccac gccacgcgca tccaggcccc cgaactcagc cttgacgtcg tcgggaccgg
     6721 tggtgaccgc gcccagacgg tgaacatctc gaccatggcc gcgatggtgt gtgctgctgc
     6781 gggagtctcg gtcgtcaagc acggcaaccg tgcggcctcg agccgctcgg gatcggccga
     6841 tgtcctcgaa gccctgggca tcgacctgac gctgcccgca gcgcgcgtgg aggcactggc
     6901 ccacgaggtc ggcatcacct tctgcttcgc ccagcagttc cacccctcga tgcgccacgt
     6961 cgccggcacc cgccgggagt tgggtgtgcc cacggccttc aacttcctcg gcccgctcac
     7021 caacccgggc cagccgacgt atgccgccgt cggcgtcgcc gacgcgcgga tggctccgat
     7081 catcgccggc gtcttcgccg gtcggggccg ggatgccgca gtgttccgcg gcgacgatgg
     7141 gctcgacgag ttgacgttgg ccacgacgtc caccgtctgg tgggtccgtt caggtgacgt
     7201 cgtcgagcac caactcgacc ccatccggtt gggcttgacc ctctcgccaa ccgaggcgct
     7261 gcgtggcggc gacgcggcat acaacgccgc agtggtgcgc gacgtgttcg gcggagcgcg
     7321 tgggccggtg cgtgacgccg tcgttctcaa cgccggtgca gccttggctg ttgccgccgc
     7381 caatgactcg gggcgcaccc ccgccgcggt tcacgccgac gtgcgagccg ggatggatcg
     7441 cgccgaagca gccttggact ccggcgccgc cgacgctctc gtgcagcgtt gggtcgaggc
     7501 gactcgcgcc tgacggctgg gcgctgagct cgggtcttgt tgccgtcttt gggcggcaac
     7561 ctagcgcacc agaccgtgga ggaatcgtta tcgcccccct cggacatggg catcgcggac
     7621 ggtccgaagg gtgtccgggc cgtgcgccct gccctgttgg cgccccatgg cgcggacggg
     7681 gagttccagg ccgatgccgg tggacaaccc tgccctcgag gcagacccct ggttcgatgc
     7741 gggtcctgcg gcctagtcga ggccgagact gaaggcggcc tcaaggtcgt gcctgctgta
     7801 ggtgcggaag gcgatgtggg tgctggtcgc agcgacgccc tcgaccttgg acagccggtc
     7861 ggcgatgacg tcggccaact gctcgtgctc acgcacccgg accatcgcca cgaggtcggc
     7921 gtcgcccgtg acggagtaga cctccgtcac gccgtcgagg gccgcgatgt cctgggcgac
     7981 ttcggggatc cgggcggcgt cgcaggtcac gaggacaatg gctgtgatca cgcgctcagc
     8041 ctagcggcgc acccgcgggg accgctcaag caccgcggcg caggtcgtcg aacgtcgcct
     8101 tcgccgcgcc cgcaccaccg acaggacacg tccattcgcc atcgagactg acgatgcgca
     8161 cacccgggct ctccagccat cgcagcacca actccgtctc ctcggggtat gccgcgggcg
     8221 ccggtcccgc gggcgggggc actgcttcgg ccgtggcccg caggctctcg acatagacga
     8281 gcgggtcggc accgcggggg gccaggctgg tcccggccag ccgcccgtgt cggacgcaca
     8341 ccaactccca ggcaccggcg tcggtgcgtc gagcggcgac gagctcgggg ctggccgcca
     8401 gcggggccag acgctgcgct cgggccaggc cgcgcaccag gtgcccgagc cgatcgcgca
     8461 gggcgcccgc gtcttcgaag cgctccgcct tggccagcac cgtcatccgc tcgcagaggg
     8521 catcaaccat ggatcggccg tcgccgacga ggagagcggc ggcagtctcg acgtgcacgg
     8581 catactccga caggctctgg cggccctcgc agggtgcgcc gcagcggccc atctcggcca
     8641 gaacgcacgc cgagcccgtg cccttcgtcg acaggcgggt cgtgcactgg cgcaggggga
     8701 ggacgtcgtg cacggcggcc accgcggcct cggcagccgc acgggaaccg aacgggccgg
     8761 cataccgggc gccggaggcg aggtcgtcgc gaacctcgcg gacgatggac agccgggggt
     8821 agggctcggc ggtgagcttg acccagaggg cgcgctcggg gcgggtcgag cggcggttgt
     8881 agcggggctt gtgctcggcg atgagccgca actcgcgcac ctgggcttcg agcgtggtct
     8941 ggcagacgat cggctggatc gaggccgcga cggacaccat ctcggccatc cgggtgcgtt
     9001 gttcggatgc agtgaaatag ctccgggcgc gcacccggat gttgcgcgaa gtcccgacat
     9061 agagcgggcg gcccttggcg tccttgaaga cgtagacccc gggagcggtc ggcaggtcgt
     9121 cggcgaggaa ccgtttgcgc cgctgcgccg tggtgacccg cgagctgtag ctggcgagct
     9181 cctcgagggt ctggaccccg acgttgccca ctcgggcgag cagcgcgtgc aggacgtcga
     9241 ccgtcgcccg ggcgtcgtgc agtgcacggt ggtcaggggt cgtgtcggca ccgaagagcg
     9301 ccgccagcga tcccaaccgc cggttggggg cctcgtcctt gctcaccaac tggcgggcca
     9361 gggtgacggt gtcgacgacc ttgaaacccg gccacgactg gccggtcgcc tccgcagcgg
     9421 ccttgaggaa ggtgatgtcg aagccggcgt tgtgcgcgac gaggacactg ccgcgggcga
     9481 actcgaggaa ggcgggcagc accgtgtcga tgcgcggcgc ttgagcgacc atgccgtcgg
     9541 agatcccggt gagcacctgg atgaacgccg ggatggggga gcctgggttg acgagggtct
     9601 ggaactcgcc gagcacctcg ccgccacgga ccttgaccgc gccgacctca gtgatggcgc
     9661 attcgttggc gctgcctccg gtcgtctcca ggtcgaccac gacaaaagtc accgcgtgca
     9721 gcggggtgcc caggtcatcg agggtgccct ggactgcgtg catgtcttcg accgtagaac
     9781 cagggtctga cagccctcgg gaggcgcgcc cgtgccaggg ggtgtcagac cctggttcta
     9841 ccgtcgacaa cgaaccggag cggactcgag aagcgagccc accgatccga gaaggagccg
     9901 acgaaatgat cgtcacatgc gcaacctgcc ccgtgcgggg gctgcggtgc gatgactgtg
     9961 tggtgacggc cctcgccacg atctccgtgg ggccgccagg tgagcgaccg ctcgacgcga
    10021 aggaacggcg cgccgtcggg ttgttcgtgt cggctgggtt gctcgacagc gggtatgccg
    10081 cgacgctcac ctcgacggtc gactccggtc gggtgggccg agtgggccgt gcggtcggct
    10141 gagcggcccg acgtgacggc ggccggcgcc gtgccgacat agcatcaggg ccgtgacggc
    10201 gttcggcagg cgatcggcct ggggcaggtg gctgccagca gccgatgtcg acggtcgccg
    10261 cgcctccctc gtgaccgcca tcgtgacagc gcacggggcc gccgtcgtga ccgctctggt
    10321 ggccggagtc gtcgtcgccc tcgtgatgtc aggctgcacg cccaacgccg cgacgccctc
    10381 gacgacggcg accgccagca cccgggcgaa cagtggggcc aacgccgggg ccgatgccgt
    10441 ggccggcgcg ggctccgagg cctccgaggc ggcccggacg gcgttgacca cgctgatggc
    10501 cgctcgatcc cgggcgttgg agacaggcga tcgaacggcc tggcttgcga cgatcgccga
    10561 cccacacggg ccggacggat cggctgagga ggcggcatac gcggggttgg tggccttggg
    10621 tgtgcgcgag gtgatcgttt ccgaggtccg caggtcggcc acccaggcca cctcggccgc
    10681 cccggcagcc gaggagtcac agcacgcaca gacgttgaca acacagacgt ggaccgggtc
    10741 gatgcggctg ggatatgcca ttcccgggtt cgaccggggg gttcggtggg tcgcacgcac
    10801 ggtcacgctc acccaggtcg ccggtcagtg gctcatccag cgctgggtcg gccccgccga
    10861 ccggtgggaa gtgttcgacc tctcgcccct tcaggtcgct cgcagcgagc gggcactggt
    10921 ggccggccca gttgcgcagg atgtgcttcg cgaccggctc gccgaggtcg aggtcgggca
    10981 ggaccgggtg gcggccgcac tggggagagc cttccccgca gtcgtcgtcg tgcccgcgac
    11041 gaccgatcag gcagcgctgc tgctgggcaa cggcgcaccg tcgagcgaac agtcgcccct
    11101 ggccggtcag gtcgcggcca ccacgcatgg acctcgggag ccgagcgcga ccgcagtggc
    11161 cgaccgggtt gtcctggatc ctcagggcat gggccgactg acaccggcgg ggcgccgcgt
    11221 cgtgctcacc cacgagttga cccatgtctc cgtgcgtgcc gggaccttgc acgacctgcc
    11281 cctgtggctc tcggagggtt tcgccgagtg ggtggccttc cgcgacgaag ggatggacgt
    11341 caccgtggtg gcggcccggc tcctgtccca ggtccgggcg tccggtccac cggcgacgtt
    11401 gccgaccccg accgatttcg ccgggacggc gggcgaccca gcggcggcat accaggagag
    11461 ttggctggcc gcctcgcgaa tcgcgaccct cgccgggccc gacgggttgg tcgggctggt
    11521 gcggcgggtc ggcggggtcc ccggcgcgtc gagcagcccg gcaggaccgg tcgaccccgc
    11581 agccgcgctg accgaggtgc ttgggcagag catgtcccag tttttggcgg gctggcagtc
    11641 cgagctcgtc accctcgcag ccgggtgatc tcgggcggac cgtcccggac gcggcgatcc
    11701 gggggctctc gggggcgata acctacctgc catggccgaa cgcaccgagt cttccatcca
    11761 cgtggacgcc aagcccggtg tcgtcctgga cgtcatcgcc gacttcgagg cgtatcccga
    11821 gtgggcggcc gaggtgaagc gggtgtccgt gctctccgag gagggtgacg gttgggccga
    11881 tcgcgtggag tacaccctcg acgcaggcgt cctcaaggac acctacacgc tggactatga
    11941 gtgggacatc tccgatgacg gcacgggggt cgtctcgtgg agcctggtcc gggccacggt
    12001 gctcaaggcg atgaacgggt cctacaccat cgcccgcgac aaggccggca gcaaggtgac
    12061 ctaccgcttg gccgtcgacc tcaacatccc catgctgggg atgctcaagc gcaaggccga
    12121 gcagcagatc gtcgatgcag cgctcaagga gctcaagaag cgcgtcgagg gctgattgtg
    12181 tcggtcgagg ggagcgaccg ggtcggctcg gtcgccgagg aagccgcgcg cttgttgggg
    12241 gtgttcgccg ccggtatgcc ggaggcgcac cttctcgccg ccggatcccg ggccgcgggg
    12301 gagacaccga cagcccacgc ctgtcccgag tgtgggcacg acccgcatgc cgcgtcatcc
    12361 ggggactcgg tatgccgcat ctgcccgatc tgccgagtcc tcaatgtcgt gaaggcgatc
    12421 agtcctgaga ccctggacaa gatcgccgat gtcgtcgacc tggtcagcga ggggctgcgt
    12481 ggcttcgccg ccacccgccg gaccgaggcc cctccgcccg cgccgacggg cggcgagcaa
    12541 ccccaggagc cggtgtgagc gcggcataca cgatcgggat cgacatcggt ggcaccaagg
    12601 tggccggggg cgtggttgac gtcgatggcg tgatcctcgc gaaggcgcgc gccgacaccc
    12661 ctcatcgatc gacctcaccg gaggtcgtcg agagcaccat cgtgggtgtc gtcgaagacc
    12721 tgatcgccca ggtgggccca ggccaggtga gcgccgtcgg catcggagcc gccgggttcg
    12781 tcggggctga tcgcgccacc gtcgtcttcg cgccgcacct gtcctggcgt caggagccgc
    12841 tgcgcgacgc cctgttcgac cggatcgggc tgcccatcac ggtcgacaac gatgccaatg
    12901 cctccagttg ggccgagtgg cggttcggcg ccggccgggg ggagagccat ctggtcatgg
    12961 tcaacctcgg gaccggtatc ggtggcggga tcgtgctcgg cggccgggtc gaacggggcc
    13021 ggttcggcat cgcgggcgag ttcggccaca tgcaggtcgt tcctgacggg ctgcgctgcg
    13081 aatgcggcaa ccgggggtgc tgggagcagt actccagcgg gaacgccctc gtccgcgagg
    13141 cccgaacgct gctgctcgcg aactctcccg tggccgacga cctgcgtgcc cgcgtcggag
    13201 gagacccggc gctgctcacc ggccctctgg tcacagaggc cgcccaggcc ggtgatcgca
    13261 cggcgatcga gttgctcgcc gacgtaggcc gctggctggg agtcgggatc gccaacctgg
    13321 cggctgcctt cgaccctggg gtgttcgtca ttggcggcgg cgtcagcgcg gcaggggagt
    13381 tgctcctcgg ccctgctcgg gagacctatc ggcgccaact caccggccgt ggctatcggc
    13441 cggaggcaaa gatcgtcgcc gcccaattgg gcaacgacgc cggggtgatc ggtgccgccg
    13501 atctggcgag ggctgaagca cagccggagg tgctcgccga ggccagtgcg ccgtctgccg
    13561 atggcgggac gcgtgaggtg gtcacgtccg ccgagtcggc tgaggcaacc tcgcacccgg
    13621 gtgcgcggcg cgtcaagtgg tggcgcaggc ggcataccga ctgacgtcgc ggcgcggatc
    13681 ggtgggtggg ctgtccagct agacctgggc gccgttgtcg tccggatcgc gctcgcctga
    13741 cccacgcaga accatcaagc cgaagccggc cacggtcaac acgatgcccg tgagcaccca
    13801 ccaccccggg tcagggtttc gcgacaccat tgccgcccag atgacgagca gcgggccgag
    13861 ggtcatcccg gcgaccgcga gccagtaggt cgcgtcgtgc accggcggca ggttcggcgg
    13921 cggcggttgg aagtgctcgt cctccggagg cggggtgtgg ccccgccagc cgaagagccg
    13981 ctcctcatcg gcgtcctctg cggccgaaaa gtgatcagtg gtggcgtccg gggcgtcgcc
    14041 cacgggcgcg gcgacgtgtg cggtggcggg gtatgccggg gggaggggtg ccggggcgag
    14101 gggtgcgggt gcggtgaggt ccgccgcgtc gccagcggcg tcgtcagcgg cgcggtcagc
    14161 gagcggctca ggtgtcgggt cgtcccaatg cgcgacgatg gaggcgaagg cggcgtcgat
    14221 ctcgttcaac ggggggagcg ccgtcgcaga agtctcctca gggccgcccg cgggctcgga
    14281 tctgccttgg tcgtccgcct cgagggcatc ctcgtcgttc acccacctag tatcagtccc
    14341 cggcacgctc cggcagactc gaaccacgcc tgcggggcca gtggggctct ccggccgggc
    14401 caccggtgct ctagagtgct gcaggccaca caggaagggc tcccgcggtg ttctactggt
    14461 tcctcaagac ggtcgtgctg ggcccgatcc tcaagctgtt gttccggccg tgggtcgagg
    14521 gcgcggagca tatccccgag cgcggggcgg cgatcctggc cagcaaccac ctgtccttct
    14581 ccgactcgat cttcctgccc atcgtcatgt cgaggcggat caccttcccg gccaagatgg
    14641 agtacttcac cggttcgggc gtcaagggtc gtctgaccgc gtggttcttc aagggcgtcg
    14701 gccagatccc gatcgaccgg tcggggggcc gggccagcga ggcggcgatc agttcggggc
    14761 tcaaggtgct gcgccgggac gaactcttcg gcatctaccc cgagggcacc cgctcacctg
    14821 acggacggct ctacaagggc aagaccggca tcgcccgaat ggcgctggag gcgggttgcc
    14881 ccgtcattcc ggtggcgatg atcggcaccg acaaggcgca gcccaccggg cagaaactgc
    14941 cgaaaatcat gcggatcggg gtccggatcg gggcaccgct ggatttcagc cgctacgaag
    15001 gcatggagaa cgaccggttc gtgctgcgat cgatcaccga cgagatcatg tacgagctca
    15061 tgctgttgtc cggccaggag tacgtcgacg tttatgcgac gtcgctgaag aagaacgtcc
    15121 tcaaggacac cgtgcagacg gccgtggcca aggcgcagga gatccagaag acggcccagg
    15181 agatccagaa gaccgcggcg gaggccgcaa aggctgccac cgaagcggct cgcgccgtca
    15241 ccgagtcggc caagaccagg gcaactcatc ctcaggaacc gttggagacc cacgaagcag
    15301 tcgaggcccc cgactccgct acatcgacgg gttcggtgga cgacgacggc ccgagttcgg
    15361 atgag
//
LOCUS       DAOMZY010000033        15355 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_3481_length_15355_cov_5.679739, whole genome shotgun sequence.
ACCESSION   DAOMZY010000033 DAOMZY010000000
VERSION     DAOMZY010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 15355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15355
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3481_length_15355_cov_5.679739"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PLK64_03285"
     CDS             complement(<1..676)
                     /locus_tag="PLK64_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007540561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV78794.1"
                     /translation="MSTASPAAVPPVPAAEGLTHRQILTILSGLMTGMFLAALDQNIV
                     GTAIKTIADELHGLSAQAWVTTAYLITSTIATPLYGKLSDIYGRKKFFMAAITIFIIG
                     SAMCSFAASMYQLAAFRAVQGIGAGGLFSLALAIVGDIVPPRERAKYQGYFLAVFGTS
                     SVLGPIIGGFFAGADSILGITGWRWVFLVNVPVGLVALLIVARTLHVSHFARPSAIDW
                     WGAFTLV"
     gene            complement(719..1216)
                     /locus_tag="PLK64_03290"
     CDS             complement(719..1216)
                     /locus_tag="PLK64_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPV78795.1"
                     /translation="MQATISPDAGPPAVTVGTGGALGAELVRLVKLLVAMRQHAPTTH
                     GAIDTTHYPVLFALANGPLRISDLAGCAHSDVSTVSRQVSFLVQHHLLAKVPDPADGR
                     AHRVSLTDEGRAAIDQALAARGQWLSAVMTDWSDEDARQFLHQVSRFTASLEAAHGRF
                     ERAAR"
     gene            1310..3352
                     /locus_tag="PLK64_03295"
     CDS             1310..3352
                     /locus_tag="PLK64_03295"
                     /inference="COORDINATES: protein motif:HMM:NF021214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HPV78796.1"
                     /translation="MSTAASDKRPLLPLAPLLATGVVALAACLVVIGVGGAAAPAAPG
                     LQDPGAVVRWGLPIIRTVHDLSAALTVGLFFLAAVAIPDRASAALARAPQWGVAAGIT
                     WVVSGLIGVVLGFANIAGTPLGSSGFFAQFQRFVWSVETLREGLISAFLAAIAVTVAA
                     MWSSRRAVLWAGIVSVVAILPLALAGHGASTVEHETAVNGLAFHLVGTALWVGGLGAL
                     VLLRPVLGKWLPIVVERYSKVAAWSLLTVALSGVVSAAVRMSGLSDLATTYGAIILAK
                     VVAIVVLGQLGLAQRTKVIARLRETPSSAPLFARLIAVELVVMGAAIGIATALARTGN
                     PSLERPAPASLAEGLTNYPEPAAPDATSWLTMWRWDWLWGTVAVVAILLYVGGVVRLM
                     RRGDRWPVGRTIVWVLGWVIFIWSVCGAPGVYGRVSFSWHMTSHMTIAMIVPILLVLA
                     APITLIARVESPRKDGTYGPREIVLGLVHSKYVAFWANPIVAGVNFVFGLIIFYYTPL
                     FELALTTHTGHVLMVIHFLLAGYLFAMVLIGVDPGPRKWAPALRLVVLFATMSFHAFF
                     GVAIQSSTSLLAPGFFSIIHIPWIPDPLLDQQQGGAIAWGIGDVPSLLLAMLLVLSWV
                     RSDDAEARRHDRQADRDHDADLTAYNARLASIAEHDAQEAARDAARLSGMPREGGS"
     gene            3358..4209
                     /locus_tag="PLK64_03300"
     CDS             3358..4209
                     /locus_tag="PLK64_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPV78797.1"
                     /translation="MRIAVVQVAHGDDERVAERVARVAATVRGLGGHDLVVLPELWPV
                     GGFDYRRWAEQAEAADGPIAAAMAQAARDAGVVLHAGSIVERGPAPGPEGKWLWNTSM
                     VFGPDGSELARYRKIHRFGFATGEPELMEAGDEVVVFDLPVGVSAGGAGADGGPATRT
                     VRAGLSTCYDLRFPELYRRQLDLGAQVLLVPAAWPAARVGHWCLFARARAVEEQCLVI
                     ACNTAGTHCGVTMGGRSVVVLPTGDVVAEAGADDEEVLSVEVDLEVIERTRAVFPVLA
                     DRRLRMP"
     gene            4481..5818
                     /locus_tag="PLK64_03305"
     CDS             4481..5818
                     /locus_tag="PLK64_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPV78798.1"
                     /translation="MAAATPADRDRWADALRVGSLLVVIVGHWLMVAVTPTGEITNTL
                     DLIPRLQPVTWVLQVMPLFFLVGGVAHAHSLGSLARRGGTGPGGYAAFIRARAARLLR
                     PTLAFLAVWVLLGVIADRTGLTSGSSWQAALVVAALVMVPQLLWFVGIYLAVAAFAPA
                     LYRAHGRWGLWAVVALCSAALLVDLARFGAGLGILGNLNFALVWLALHQCGFAWRDGS
                     LTKRVAFVLVGVGVSGLALSIAVGPYPVSMVGLPGDEISNMSPPTVALLCQGLALIGI
                     AALARGPMARVLARPRVWRGVVTAGAFAMTAFLWHLTALLFTLLSAGALGISLPEVGS
                     AAWWWSRPVWFAVLAIPTAALVAIFIRFDRGPRSAAGVVTGQPRWVDWVAVAGVIVTV
                     FGILMVSIVGVDILGNRAQFFLFGMLTPGQAFLVLLLGLALLRLAAPRAVSRT"
     assembly_gap    5939..6038
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6091..7026)
                     /locus_tag="PLK64_03310"
     CDS             complement(6091..7026)
                     /locus_tag="PLK64_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78799.1"
                     /translation="MLDNPTTDVSQLAKTLKMKRGEVAAELDRLAALRLVTTDEGGVN
                     VVSPVAPDQAIESLLAQQERRLAQMQELVDTSRQNVAHYVEAFVDSRVRRDGHDLVEI
                     IDEAEVVRSRLYQLVREARVSAVTIHGGAPLPSEAIESSRRLDRELLERKVVLRAIVA
                     TPSLTSEAWRTHLVEASSIGMQVRVHPSPGPHTIVIDGSVALIPRTSRPGALILHGPD
                     LVAPVAQLFTQVWNAAQPWDDIVNLGAEGEFSEARLRQVVSLLAQGLKDDAIARKTAT
                     SVRTVRRLVAAATAALQAESRFQAGVEALRRGWVD"
     gene            complement(7227..9095)
                     /locus_tag="PLK64_03315"
     CDS             complement(7227..9095)
                     /locus_tag="PLK64_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPV78800.1"
                     /translation="MGHYKANVRDLEFNLFEVFGRQEILGKGIYADVDVEAAKDILGE
                     VARLAENELAASLVDSDRNPPVYDPVTCSVTIPESFKKSYRAYLDGEWGRLDTPVELG
                     GMAVPPSLRWAIAEMICGANPAVHMYGATFSFAKLLWFLGTPEQKKLAKLIIDNAWHT
                     TMVLTEPDAGSDVGAGRTKAVQQPDGTWHITGVKRFITSAESDMCDNIVHFVLARPEG
                     AVVGTKGLSLFIVPKFHVDVETGVLGERNGAYVTNVEHKMGLKVSTTCEVTFGEKQPA
                     VGTLLGDVHDGIAQMFHIIEGARMFVGTKAIATLSAGYLNALDYAKQRVQGADLTTPA
                     KDAPRVTITHHPDVRRSLMLQKAYAEGLRALVIYAATFQDVVDVETAGVPSVHPEKGS
                     PADVASRMSDLLLPIVKGLGSERAWVLLGTESLQTFGGSGFLQEYPLEQYVRDAKIDT
                     LYEGTTAIQGLDFFFRKIVRDHGQSMTGLSMQIAQFAKDLGAQAGYFDSEREALGKAL
                     ENVQGIVGYMAGAAFQSDPRTGGDVKNLYKVGQNSTRLLLGAGDLIVGWLLMRQAEVA
                     KAALDAGASPKDVDFYTGKIAAAKFFVHQVLPRLAAERAMAESTDLSLMDVPEAAF"
     gene            complement(9469..10686)
                     /locus_tag="PLK64_03320"
     CDS             complement(9469..10686)
                     /locus_tag="PLK64_03320"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HPV78801.1"
                     /translation="MHAFPTTLPGTQAEVKMWAHEHEIDSAALQQLRNIASLPWVVGV
                     RVMPDVHLGKGATVGSVIAMSQAVAPAAVGVDIGCGMAAVRTSLRLEDLPDDLHPLRL
                     EIEAAVPVGFAGHQHPVDPTSLGMPRNEFRAEGWGRFWDAFDDLDPRIQDQAKKAHQQ
                     MGSLGGGNHFIEVCSDDDGAIWVMLHSGSRGIGNLLAQAHMTTARGLPHNDGIPDRDL
                     AVFLAGTPEMAAYRRDLEWAQDYALRNRAVMMALVQRVVGAHFAARGVPVEWGQTIQC
                     HHNYVAQETYDGIDVLVTRKGAIRAGAGDLGLIPGSMGTGSFVVRGLGNVDSYCSASH
                     GAGRRMSRSAAKKRFSEADLAAQTAGVECRKDAGVVDEIPGAYKDLDSVIAAQSDLVE
                     VVTRLTTLLCVKG"
     gene            complement(10872..11837)
                     /locus_tag="PLK64_03325"
     CDS             complement(10872..11837)
                     /locus_tag="PLK64_03325"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HPV78802.1"
                     /translation="MAIVAVTNRIPEVGLAKLRAEHEVRAWEHSECIPREEALRLIAG
                     ADAVLTLLTERVDGEFLDAAGPQLKVVANVAVGYNNIDVAACEAHGVIATNTPRVLTE
                     TTADTAFGLMLMVTRRFGEGERIIRSQTPWQWGMFYLLGMGLQGKTIGIVGMGQIGVA
                     MARRAKAFGMTIEYADDYDPPAAALEGLDARKVSLDELFETADVVSLHCPYGPSTHHL
                     VDAARLRSMKHTAYLVNSARGPIVDEDALVAALRAGEIAGAGLDVFENEPAVHPGLLD
                     LENVVLLPHLGSATAETRAAMAELAADNALAVLRGDPPITPVTKI"
     gene            complement(11843..13036)
                     /locus_tag="PLK64_03330"
     CDS             complement(11843..13036)
                     /locus_tag="PLK64_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPV78803.1"
                     /translation="MPFAERISRLGTETAFAVSLAAAAWGAQGHRIYPFHLGDIDLPT
                     PANIVEAMDRAIADGKTGYCPGAGIMPLREALAADVAGRRGLDLSADNVTIHPGGKPV
                     ITKFVQALIEFGDEVLYPNPGYPIYESQIEFIGGKPLAYRYLPTDTGFEIDLDQVRSL
                     ITPATKAIVYNNLQNPISAESSQAEMEAIAQIAIENDLWVLADEAYYEMRYSGTSSSI
                     ASLPGMAERTVLLYTFSKKFAMTGWRLGAAIAPAELSSVFSKLNTNNESCTTHFVQWA
                     GVEAVTGDQSGANAIRETLRGRRDAAYEMLVSMPGVRLSKPNATFYLFPDVTEAMAAK
                     GIDSLPEFADRALKETGVSFCTRLHFGRPQPGEDRRYVRFAYSGLPESDIREGLGILK
                     QWIEG"
     gene            complement(13105..14055)
                     /locus_tag="PLK64_03335"
     CDS             complement(13105..14055)
                     /locus_tag="PLK64_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010635806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HPV78804.1"
                     /translation="MRIVVALGGNALLRRGEPPTAENQRTNVHAAALALAPLARDHEL
                     VITHGNGPQVGLLAVQAAALPGVAAYPLDLLGAQTDGMIGYLIEQELGNLLPENQPLA
                     TLLTMTEVDPDDPAFADPTKFVGSVYDEATARAAAAEHGWTVKPDGPHWRRVVASPKP
                     LRIFEIRPVDLLLRAGCVVICAGGGGIPTMFERDAQGRRSNVLAGVEAVVDKDLASGV
                     LARELGADAFVMATDVPAVIDHWGMPSARQIAAARPEWLLAQGFPAGSMGPKVEAAAA
                     FATETGRPAYIGALEVIGDLLAGRAGTLVSTSLAEPTRLR"
     gene            14174..15310
                     /locus_tag="PLK64_03340"
     CDS             14174..15310
                     /locus_tag="PLK64_03340"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HPV78805.1"
                     /translation="MAAAPGHIVIACDKFKGSLTAAQVVEAIRDGIASAAPGREVRAV
                     LVADGGDGTLAAAESVGFRHVPVTVAGPTGSPNLSGYAVRAGTAVVELADACGIVHLP
                     GGVKAPLTATSRGLGQVLAAALDDPSVTSVVMGIGGSASNDGGAGMLAALGARLLDAG
                     GRELPDGGGALVDLAALDLSGLHLRLREVSLTVACDVNNPLLGEPGAVAIFGPQKGAG
                     EAERVVLEAGLARFASLVTEAMRRDDTGLPGAGAAGGVGYAAQAVLGAAMRPGVELVL
                     ELMGFEALCEGASLVVTGEGSLDRQTLLGKTPAGVARAAQAHGIPVVAVCGRSLLSAE
                     DVAASGIAQVYALTDLEPDPAVCMSDAARLLRALSAQMAHDWLS"
BASE COUNT         2130 a         5031 c         5473 g         2621 t
ORIGIN      
        1 cgacgagggt gaacgcgccc caccagtcga tggccgacgg ccgggcgaag tgggagacgt
       61 gcagggtccg ggcgacgatg agcagggcaa cgaggccgac cgggacgttg acaaggaaca
      121 cccagcgcca tccggtgatc ccgaggatcg agtcggcccc ggcgaagaac ccgccgatga
      181 tcgggccgag caccgacgac gtgccgaaga cggccaggaa gtagccctgg tatttggcgc
      241 gctcccgcgg cggcacgatg tcgccgacga tggccagcgc gagcgagaag agaccgccgg
      301 ccccgatgcc ctgcacggcg cggaaggccg ccagttggta catcgacgcg gcgaacgagc
      361 acatggccga gccgatgatg aagatcgtga tggccgccat gaagaacttc ttgcggccgt
      421 agatgtccga caacttgccg tagagcggcg tggcaatggt cgaggtgatg aggtaggccg
      481 tcgtcaccca ggcttgggcc gacaacccgt gcagctcgtc ggcgatcgtc ttgatggcgg
      541 tgccaacaat gttctggtcg agcgcggcga ggaacatccc cgtcatcagg ccggacagga
      601 tggtgaggat ctgccgatgg gtgaggcctt cggcggctgg gacgggcggc acggccgcgg
      661 gactagcggt ggacacgatg ctcctgtggt gggttacgac ggtgcagagg tatgccggtc
      721 agcgcgccgc gcgctcgaag cgcccgtggg cggcttccag cgacgcggtg aacctgctga
      781 cctggtgaag gaattggcga gcgtcctcgt cggaccagtc ggtcatgacg gcggacagcc
      841 actggccgcg ggcggcgagg gcctggtcga tggcggctcg tccctcgtcg gtgagggaga
      901 cccggtgggc ccgtccgtcg gcggggtcgg gcaccttggc gagcaggtgg tgctggacga
      961 ggaaggacac ctgacggctc accgttgaga catcggaatg ggcgcacccg gcgagatcgg
     1021 agatccgcag cggtccgttc gcgagggcga acaggaccgg gtagtgcgtc gtgtcgatcg
     1081 ctccgtgggt ggtgggtgcg tgctggcgca tcgcgacgag gagcttgacc agtcggacga
     1141 gctcggcacc gagggcccct ccggtgccga ctgtcacggc cggcggcccg gcgtccgggg
     1201 agatagttgc ttgcaccacg caactataaa ctgtttgcac gccgcaacca aatggtcggg
     1261 gaggggcacc gggtgctgct gcgcgaggga acagacctag ggtggagtca tgtccactgc
     1321 tgcctcggac aagcgccctc tcctgccgtt ggccccgctg ctcgcgaccg gagtggtcgc
     1381 gctcgcggcg tgccttgtcg tcatcggggt tggaggggcg gccgctcccg ctgctcccgg
     1441 attacaggac ccgggagccg tcgtccggtg gggattgccg atcatccgga cggtccatga
     1501 cctgtctgcc gctctgaccg tcgggctgtt cttcctggcc gcggtcgcga tccccgaccg
     1561 cgcctccgca gcgttggccc gggcccctca gtggggcgtc gcggccggca tcacgtgggt
     1621 ggtctccggg ctgatcggtg tggtcctggg ctttgccaac atcgccggca cgccgctggg
     1681 gtcctcggga ttcttcgccc agttccagcg cttcgtctgg tcggtcgaga cgttgcgcga
     1741 gggcctcatc agcgcgttcc tcgccgcgat cgcggtcacc gtcgcagcga tgtggagcag
     1801 ccgacgggcc gtgctctggg cagggatcgt ctcagtcgtc gccatcctcc cgttggccct
     1861 ggccggccac ggcgcgagca cggtcgagca cgagactgcc gtcaacgggc tggccttcca
     1921 cctcgtcgga accgcgctct gggtcggcgg attgggcgcg ctggtcctgc ttcggccggt
     1981 attgggcaag tggctgccga tcgtcgtcga gcgctactcc aaggtggccg cctggtcgct
     2041 gctcaccgtt gctctctccg gtgtggtgag cgcggccgtc cggatgtcag ggctcagtga
     2101 cctggccacg acctacggcg cgatcatcct ggccaaggtc gtggcgatcg tcgtcctggg
     2161 ccaactgggc ctggctcagc ggaccaaggt gatcgcgcgg ctgcgcgaga cgccgtcgtc
     2221 cgcgcccctt ttcgcccgtc tcatcgcggt cgagttggtc gtcatgggcg cggccatcgg
     2281 catcgccacg gccctggccc gcaccggcaa ccccagcctg gagcgtccgg ccccggcaag
     2341 cctggccgag gggctgacca actaccccga gccggccgct cctgatgcca cgtcgtggct
     2401 gacgatgtgg cgctgggact ggctttgggg gacggtcgcc gtcgtggcga tcctgctcta
     2461 cgtcggtggt gtcgtgcgtc tgatgcgccg cggcgaccgg tggccggtgg gccgcaccat
     2521 cgtgtgggtg ttgggctggg tcatcttcat ctggtcagtg tgtggcgcgc cgggcgtcta
     2581 tgggcgggtg tccttctcct ggcacatgac cagccacatg acgatcgcca tgatcgtccc
     2641 gatcctgctt gttctcgcgg cgcccatcac cctcatcgcc cgcgtggagt cgcctcgcaa
     2701 ggacggcacc tatggcccac gagagatcgt cctcggcctc gtccactcga aatacgttgc
     2761 cttctgggcc aatccgatcg tggctggggt caacttcgtc ttcgggctga tcatcttcta
     2821 ctacacgccg ctgttcgagt tggccctcac gacgcacacc ggccacgtcc tcatggtcat
     2881 ccacttcctc ctggccgggt atctcttcgc catggtgctc atcggcgtcg acccaggccc
     2941 ccgcaagtgg gcgccggccc tgcgcctggt cgtgttgttc gcgacgatgt ccttccacgc
     3001 gttcttcggc gtcgccatcc agtcgagcac gtcgctgctc gcgccggggt tcttctcgat
     3061 catccacatc ccgtggatcc ccgacccgct gctggatcag caacagggcg gagccatcgc
     3121 gtggggcatc ggcgatgtcc cctccctgct gctcgccatg ctgctggtgc tgtcctgggt
     3181 tcgctcggac gacgccgagg cacgccggca cgaccgccaa gccgaccgtg accacgatgc
     3241 cgacctcacg gcatacaacg cgcgcctggc gagcatcgct gaacatgacg cgcaggaggc
     3301 cgcacgtgac gcggcccgcc tctccggtat gccgcgcgag ggcggctcat gaccccgctg
     3361 cggatcgcgg tcgtccaggt cgcccacggc gacgacgaga gggtcgccga gcgggtcgcc
     3421 cgggtcgcgg cgacggtgcg aggactgggc gggcacgacc tggtggtgtt gcctgagctg
     3481 tggccggtgg gcgggttcga ctaccggcgg tgggcggagc aggccgaggc cgctgacggg
     3541 ccgatcgcgg cggcgatggc gcaggccgcg cgcgatgcgg gcgtggtgtt gcacgccggg
     3601 tcgatcgtcg agcgcggacc ggcgcccggg ccggagggca agtggctgtg gaacacctcg
     3661 atggtgtttg gcccggacgg gtcggagttg gcgcgctatc gcaagatcca ccggttcggg
     3721 ttcgcgacgg gggagccgga gctcatggag gccggcgatg aggtggtcgt gttcgacctg
     3781 ccggtgggtg tgagcgctgg cggtgctggt gctgatggtg gacctgcgac ccggacggtc
     3841 cgggccgggt tgtccacctg ttacgacctg cgcttccccg agctctatcg ccgccaactc
     3901 gacctcggcg cgcaggtgtt gctcgtccct gcggcctggc cggctgctcg ggtcgggcac
     3961 tggtgcctgt tcgcgcgggc gcgagccgtc gaggagcaat gcctcgtcat cgcgtgcaac
     4021 acggctggca cccactgcgg ggtgacgatg ggcggtcggt cggtggtcgt gcttcccacc
     4081 ggcgacgtgg ttgccgaggc cggtgctgac gacgaagagg tgctgtccgt cgaggtcgac
     4141 ctggaggtca tcgagcgcac ccgggccgtg ttcccggtgc tcgccgaccg tcgcctccgg
     4201 atgccctgac cgcggatcct ccgcagagtc gcgccatagt ccctcataca agcgtcacac
     4261 ctctcgccgg gtcgaggtat tccgccatca cctgacgatg gcggaatacc tcgacccagc
     4321 cgggaagccc gccagcttga ggcctcggag ggtgccatat gggacagtgg gccccgtgag
     4381 ttcgccagct acgcctcccg cgcccgagtc tccggccccg aatcttgggt cgaccgttgc
     4441 gtcggccgct ccatcagacg ctccgtcgac cgttgcgtcg gtggccgcgg ccacgcccgc
     4501 tgatcgcgac cggtgggccg atgccttacg cgtcggctcc ctgctcgtcg tcatcgtcgg
     4561 gcactggctc atggtggccg tcacaccaac cggcgagatc accaacaccc tcgacctcat
     4621 ccccaggctg cagccggtca cctgggtcct gcaggtcatg ccactgttct tcctcgtggg
     4681 cggcgtcgcg cacgcccact cgcttggctc cctcgcccgc cgcggcggca ccggtcccgg
     4741 agggtatgcc gcgttcattc gggcccgggc cgcgcgcctg ttgcgcccaa cgcttgcgtt
     4801 ccttgccgtg tgggtcctgc tcggtgtcat cgccgaccgc accgggctga cgtcggggtc
     4861 ctcgtggcag gcggcgctcg tcgtggcagc gctcgtcatg gttccgcagt tgctgtggtt
     4921 cgtcgggatc tacctggccg tggcggcctt cgcccccgcg ctctatcgcg cccacggtcg
     4981 atgggggctc tgggcggtcg ttgccctgtg ctcagcagcg ctgctcgtcg atctcgcgag
     5041 gttcggcgca ggcctgggca tcctcggaaa cctcaacttc gcgctcgtct ggttggcgct
     5101 gcaccagtgc gggttcgcct ggcgcgacgg ctcgttgacc aagcgggtgg ccttcgtgct
     5161 cgtcggcgtc ggagtctcgg ggctcgcgct ctcgatcgcc gtggggccct atccggtgtc
     5221 gatggtcggg ctgcccggcg acgagatctc gaacatgtcc ccgccgacgg ttgccctgct
     5281 gtgccagggc ctggcgctca tcgggatcgc ggcgttggcg cgtggcccga tggcccgagt
     5341 gctggcgcgc ccacgggtgt ggcgcggtgt ggtcaccgcg ggtgcgttcg cgatgaccgc
     5401 cttcctctgg catctgacgg cgctgctctt caccctgttg agcgccgggg cgctcggcat
     5461 ctcgttgcct gaggtgggca gcgcagcctg gtggtggagt cggccggtgt ggttcgccgt
     5521 gctcgcgatc ccgaccgctg ccctggtcgc gatcttcatc cggttcgacc gcggtcctcg
     5581 ctcggcggcc ggggtggtca cggggcagcc gcgttgggtc gactgggtcg cggtcgctgg
     5641 ggtcatcgtg acggtcttcg gcattctcat ggtgtcgatc gtcggcgtcg acatcctcgg
     5701 caaccgggcg cagttcttcc tcttcgggat gctgaccccc ggccaggcgt tcctcgtcct
     5761 gctgctcggg ttggccctgc tgcgcctagc cgccccacgc gcggtttctc gcacctgagt
     5821 ctcctcgtgg tcgacggcag gggtatgccg cggccagacc atccgcgctc cctcgctgcg
     5881 ctcgctcgcg cttcccgcca gacccgccat ggccgcggca tacccctgcc gcctcggcnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg cggcataccc ctgccgcctc
     6061 ggcagcgcgg gtcagtcgat gcggcgcggg tcagtcgacc cagccgcggc ggagcgcttc
     6121 gactccggcc tggaagcggc tctcggcctg caacgccgcg gtggccgctg ccaccagtcg
     6181 gcgcactgtc cggaccgagg tggctgtctt gcgggcgatg gcatcgtcct tgagcccctg
     6241 ggccaacagg ctgacgacct gacgaaggcg ggcctcggag aactcgccct cggcacccag
     6301 gttgacgatg tcgtcccagg gctgcgcggc gttccacacc tgggtgaaca gttgcgcgac
     6361 cggcgcgacg aggtccggtc cgtgcaggat caacgcaccc ggccggctgg tccgtgggat
     6421 gagcgcaacg gagccgtcga tgacgatcgt gtgcggccct gggctggggt ggacccgcac
     6481 ctgcatcccg atcgaggacg cctcgaccag gtgggtgcgc caggcttccg aggtcagcga
     6541 tggggtcgcg acaatggccc gaaggacaac cttccgctcg agcaactcgc gatccagccg
     6601 acgcgacgat tcgatcgcct cgctcggaag cggtgcccca ccgtgaatgg tgacggcact
     6661 cacccgcgct tcgcggacga gttggtagag gcgtgagcgc acgacctccg cttcgtcgat
     6721 gatttcaact aagtcgtggc cgtcccgtcg aacgcgactg tcgacgaaag cttcgacgta
     6781 atgcgccacg ttttgacgag aagtgtcgac taactcctgc atctgcgcga ggcggcgttc
     6841 ctgttgcgcg agcagcgatt cgatggcttg gtcgggagcg acgggactga cgacgttgac
     6901 gccgccctcg tcggtggtga cgagcctgag cgcggctagc cgatcgagct cggcagcgac
     6961 ctcgccgcgc ttcatcttca gcgtcttggc gagttgcgag acatctgtgg tcgggttgtc
     7021 aagcatggct cgataaaccc gttccgtcgt tccgtgaacg ccaaagatct ctagcatgtc
     7081 ggcccctgat cgactgttgt gccccaccgt agttggaacc gttcgatggc gctcagtgac
     7141 cggtgccggg tgcgaggtca ccacacagcg gcgacgcggc ataccaggga tcaggtatgc
     7201 cgcgtcgcgc gcttcgtggg gtgaggctag aaggcggcct ccgggacgtc catgagcgac
     7261 aggtcagtcg actcggccat ggcgcgctcg gcggccaggc gcggcaggac ctggtggacg
     7321 aagaacttcg cggcggcgat cttgccggtg tagaagtcga cgtccttggg ggaggcgccc
     7381 gcgtcgagcg cggccttggc gacctcggcc tggcgcatga gcagccagcc gacgatgagg
     7441 tcaccggcac cgagcaacag gcgggtggag ttctggccga ccttgtagag gttcttgacg
     7501 tcgccgccgg tgcgggggtc ggactggaag gcggcgccgg ccatgtaacc gacgatgccc
     7561 tggacgttct ccagagcctt gccgagagcc tcgcgctcgg agtcgaagta gcccgcctgc
     7621 gcgccgaggt ccttggcgaa ctgggcgatc tgcatcgaca agccagtcat cgactgcccg
     7681 tggtcgcgga cgatcttgcg gaagaagaag tccagaccct ggatcgccgt ggtgccttcg
     7741 tagagggtgt cgatcttggc gtcgcggacg tattgctcga gcgggtattc ctgcaggaag
     7801 cccgagccgc cgaaggtctg caacgactcg gtgcccagca gcacccaggc gcgctcggag
     7861 ccgaggccct tgacgatcgg caggagcagg tcgctcatcc gcgaggcgac gtcggccggc
     7921 gaccccttct cggggtggac cgacgggacg cctgcggtct cgacatcgac gacgtcctgg
     7981 aacgtcgcgg cgtagatgac gagcgcacgc aacccctcgg catacgcctt ctgcagcatg
     8041 agcgaacggc gcacgtcggg gtggtgggtg atggtgaccc gcggcgcgtc cttggccggc
     8101 gtcgtgaggt cggcgccctg gacgcgctgc ttggcgtagt cgagcgcgtt gaggtaaccg
     8161 gccgagagtg tggcaattgc cttggtgccc acgaacattc gcgcaccctc gatgatgtgg
     8221 aacatctggg cgatgccgtc gtggacgtcg ccgaggaggg tgccgacggc gggctgcttc
     8281 tcgccgaagg tgacctcgca ggtggtcgag accttgaggc ccatcttgtg ctcgacgttg
     8341 gtgacgtaag cgccgttgcg ctcgccgagc accccggtct cgacgtcgac gtggaacttg
     8401 gggacgatga acaggctcag gcccttggtg ccgacgaccg caccctcggg gcgggccagg
     8461 acgaagtgga cgatgttgtc gcacatgtcc gactcggccg acgtgatgaa ccgcttgacg
     8521 ccggtgatgt gccacgtgcc atcgggctgc tggaccgcct tggtgcggcc agcgccgacg
     8581 tcggagcctg cgtcgggctc ggtgaggacc atcgtcgtgt gccaggcgtt gtcgatgatg
     8641 agcttggcga gcttcttctg ctcgggcgtg ccgaggaacc acagcagctt ggcgaacgag
     8701 aaggtcgcgc catacatgtg aacggcaggg ttggcgccgc agatcatctc ggcgatggcc
     8761 caccgcagag agggcggcac ggccatcccg ccgagctcga cgggggtgtc cagccggccc
     8821 cattcgccgt cgaggtaggc gcgataggac ttcttgaacg actcagggat cgtcaccgag
     8881 caggtgaccg ggtcatagac cggcgggttg cggtcggagt cgaccaggct cgcggccagc
     8941 tcgttctcgg ccaggcgggc cacctcgccg aggatgtcct tggcggcttc gacatcgaca
     9001 tcggcgtaga tccccttgcc gaggatctcc tggcgaccga aaacctcgaa gaggttgaac
     9061 tccaggtcgc gcacgttggc cttgtagtga cccatgacac tgatccttcg gtgggtagag
     9121 cgggatgccg ctggggccca gtggttgcgt ctagtttgct actggccggt aacaaaacgc
     9181 aaggggtatg ccgtgtgccc gccgtcacgg gggtccttgg cctccgggcg cccgatgggt
     9241 ctggcccggc ctgctgagtt tcgccgccat ggcggtcaaa gtggtggctg gcagtcagat
     9301 ctgggggtca cctgcgagct tcgccgccat ggcggtcaaa cggtcggcct cctgaggccc
     9361 acgtgcgcgg catacctcgg ccgcctcagc cgcctcagcg ccgaaaggtc gacgatcttt
     9421 cgcttgctcg acgatgtgtc atcgagcgcg caaaagatcg tcgacctttc agcccttgac
     9481 gcagagcagc gtcgtcagcc gagtgacgac ctcgaccaag tcggactgcg cggcgatgac
     9541 cgagtcgagg tccttgtagg cgccagggat ctcatcgacc accccggcat ctttccggca
     9601 ctcgacaccc gccgtctgcg ccgcgaggtc ggcctcgctg aaccgcttct tcgcagcgct
     9661 ccgcgacatc cgccgacctg cgccgtgcga cgccgagcag taggagtcga cgttgccgag
     9721 gcctcgcacg acgaacgacc ccgtgcccat cgagccaggg ataagcccga ggtcaccggc
     9781 gccggcgcgg atcgctccct tgcgggtgac gagcacgtcg atgccgtcgt aggtctcctg
     9841 cgccacgtag ttgtggtggc actggatcgt ctgaccccac tcgaccggca ccccgcgcgc
     9901 ggcgaaatgc gcaccgacga cccgctgcac gagcgccatc atgaccgccc ggttgcgcag
     9961 cgcgtagtcc tgcgcccact ccagatcccg ccggtatgcc gccatctccg gcgtgcccgc
    10021 aaggaacacc gcgaggtccc ggtcggggat gccgtcgttg tgcggcaagc cccgagccgt
    10081 cgtcatgtgt gcctgcgcga gcagattgcc gatgcctcgc gagccagagt ggagcatgac
    10141 ccagatcgcg ccgtcgtcgt cggagcacac ctcgatgaag tgatttccgc cgccgagcga
    10201 ccccatctgc tggtgcgcct tcttggcctg gtcctggatc cgcgggtcca ggtcgtcgaa
    10261 ggcgtcccag aaccggcccc agccctccgc gcgaaactcg ttgcgcggca taccaagtga
    10321 cgtggggtcg accgggtgct ggtgacctgc gaagccgacg gggaccgccg cttcgatctc
    10381 gagtcgcaac gggtgcaggt cgtcggggag gtcttcgagg cgcagcgacg tgcggaccgc
    10441 cgccatgccg cagccgatgt cgacgccgac cgcagccggc gcgaccgcct ggctcatcgc
    10501 gatgaccgag ccgaccgtcg cacccttgcc gaggtggacg tcgggcatga cgcgcacgcc
    10561 gacgacccac ggcagcgacg cgatgttgcg cagctgctgg agggcggccg agtcgatctc
    10621 gtgctcgtgg gcccacatct tcacctcggc ctgggtgccg ggcagcgtgg tcgggaatgc
    10681 gtgcatcggt ccaggttagg gaggcggctc gacccgccgc acgtggttat ccagccggcg
    10741 cccgaggtat gccgtgccgc gacagccaaa aggtcgacga tctttcggcg gctcaacgac
    10801 atatcgtcga gcgtgcagaa gatcgtcgac ctttcggtgc tggggccgtg cccggcggcc
    10861 ggtggcgctg gtcagatctt ggtcaccggg gtgatcgggg ggtcgccgcg caggacggcc
    10921 agcgcgttgt cggcggcgag ctcggccatc gcggcgcggg tctcggcggt cgccgacccg
    10981 aggtgcggca gcaggacgac gttctcgagg tcgagcagcc ccggatgcac ggccggttcg
    11041 ttctcgaaca cgtccagccc agcgcccgcg atctcgcccg cgcgcaacgc cgccaccagc
    11101 gcgtcctcgt cgacgatcgg tccgcgggcc gagttgacga ggtatgccgt gtgcttcatt
    11161 gagcgcagcc gcgcggcgtc gacgaggtga tgggtcgacg gcccgtaggg gcagtgcagc
    11221 gacacgacgt cggccgtctc gaagagctcg tcgagcgaca ccttgcgggc gtccaaccct
    11281 tcaagggccg cggccggcgg gtcgtagtca tcggcatact cgatggtcat gccgaacgcc
    11341 ttggcacgcc gggccatagc gaccccgatc tgacccatgc cgacgatgcc aatcgtcttg
    11401 ccctgcaacc ccattcccag caggtagaac atgccccact gccacggggt ctgcgaccgg
    11461 atgatccgct cgccctcgcc gaagcgccgc gtcaccatga gcatgagccc gaacgcggtg
    11521 tccgcggtcg tctcggtcag cacgcgcggc gtgttggtcg cgatgacgcc atgtgcctcg
    11581 cacgccgcca cgtcgatgtt gttgtagccc acagcgacgt tcgcaacgac cttcaactgc
    11641 ggcccggcag cgtcgaggaa ctccccgtcg acccgctcgg tgagcagcgt caacaccgca
    11701 tcagcgccgg cgatcaggcg tagcgcctcc tcgcgcggga tgcactccga gtgctcccag
    11761 gcgcgcacct cgtgctcggc gcgcaacttc gccaagccca cctcgggaat ccggttggta
    11821 acagccacaa ttgccatgtc agtcagccct cgatccattg cttgaggatg cccagccctt
    11881 cgcggatgtc cgactcaggc aaccccgagt aggcgaaccg cacatagcga cgatcctcgc
    11941 ccggttgcgg ccgaccgaag tgcagccgcg tgcagaacga cacgccagtc tccttaagcg
    12001 cgcggtcggc gaactcgggc aggctgtcaa ttcccttggc ggccatggct tctgtgacgt
    12061 ctgggaagag gtagaaggtg gcgttcggct tgctcaggcg gacacccggc atactcacga
    12121 gcatctcgta tgccgcgtcg cggcgaccgc gcagcgtctc ccggatcgcg ttggcgccgg
    12181 actggtcgcc ggtgaccgcc tcgacgcccg cccactggac gaagtgcgtc gtgcacgact
    12241 cgttgttggt gttgagcttg ctgaagaccg acgacaactc ggccggcgca atcgcggccc
    12301 ccaaccgcca cccggtcatc gcgaacttct tggagaacgt gtagagcagg acggtccgct
    12361 cggccatgcc gggcagcgac gcgatcgagg acgaggtgcc cgagtagcgc atctcgtagt
    12421 aagcctcgtc ggcgagcacc cagaggtcgt tctcgatggc gatctgggcg atggcctcca
    12481 tctcggcctg gctggactcg gcggagatgg ggttctgcag gttgttgtag acgatcgcct
    12541 tggtcgccgg cgtgatgagc gagcgcacct ggtcgaggtc gatctcgaag ccggtgtccg
    12601 tgggcagata gcgataggcc agcggcttgc cgccgatgaa ctcgatctgg ctctcataga
    12661 tggggtagcc cgggttggga tagagcacct cgtcgccgaa ctcgatgagc gcctgaacga
    12721 acttggtgat gaccggcttg ccgcccgggt ggatcgtgac gttgtcggcg ctgaggtcca
    12781 gcccgcgccg cccggcgacg tccgcggcga gcgcctcgcg cagcggcatg atgccggcgc
    12841 cggggcagta gcccgtcttg ccgtcggcga tcgcccggtc catcgcctcg acgatgttcg
    12901 cgggcgtcgg caggtcgatg tcgccgaggt ggaaggggta gatgcggtga ccctgtgctc
    12961 cccaggccgc ggctgccagc gagacggcga aggcggtctc ggtcccgagt cgactgatgc
    13021 gttctgcgaa tggcacttcg gtgtgtccct tcgtgtggcg gagcgaggac ccggggtgcc
    13081 gggtcgcccg tgctgtcatc tacttcatcg gagcctggtg ggctccgcaa gactcgtgct
    13141 gacgagggtc ccggctcgac ccgcaagcag gtcgccgatc acctcgagcg ccccgatgta
    13201 tgccggtcgg ccggtttcgg tggcgaaggc cgccgccgcc tcgaccttcg gtcccatcga
    13261 gccggccgga aacccttggg cgagtagcca ctccgggcgc gcggcggcga tctggcgggc
    13321 ggacggcata ccccagtggt cgatgacggc ggggacgtcc gtggccatga cgaaggcgtc
    13381 cgcgcccaac tcgcgggcga gcacgcccga ggcgaggtcc ttgtcgacaa cggcctccac
    13441 gcccgcaagg acgttggacc ggcgcccctg cgcatcgcgc tcgaacatcg tcggtatgcc
    13501 gcccccgccg gcgcagatga ccacgcaccc cgcgcgcagg agcaggtcca caggtcgaat
    13561 ctcgaagatc cgcaggggtt ttggcgaggc gacgacccgg cgccagtgtg gcccatcggg
    13621 tttcacggtc cacccatgtt cggcagcggc cgcgcgggcg gtcgcctcgt catagaccga
    13681 gccgacgaac ttcgtcggat cggcgaaggc cgggtcgtcg gggtcgacct cggtcatcgt
    13741 caacagcgtt gccaggggct ggttttcggg gaggaggttg cccagctcct gctcgatgag
    13801 gtagccgatc atcccgtcgg tctgcgcccc gagcaggtcg agggggtagg ccgccacacc
    13861 cggcagagcc gccgcctgca ccgcgagcag cccgacctgg gggccgttgc cgtgggtgat
    13921 gacgagctcg tgatcgcgag ccaggggagc gagggcgagg gcggcggcgt ggacgttcgt
    13981 gcgctggttc tcggccgtcg gaggctcgcc ccgacgcagc agggcattgc cgccgagggc
    14041 gacgacgatc cgcattccgg gggcctcctt gtccctgcct gcgagggtaa ctctgcggcg
    14101 gcataccgcg agggactctc gtcccaatcg gtgagggggt ttgcgccggg cgggcgctgg
    14161 ctagtgtgtg ggtatggccg cagccccggg gcacatcgtc attgcgtgcg acaagttcaa
    14221 ggggtcgctc acggcggccc aggtcgtcga ggcgatccgc gacgggatcg cgtctgccgc
    14281 ccccggccgc gaggtgcgag ctgtgctcgt cgccgatggt ggcgatggga cgctcgccgc
    14341 ggccgagtcg gtgggcttcc ggcacgtccc cgtgaccgtc gcgggcccca cggggtcacc
    14401 caacctcagc gggtatgccg tgcgcgccgg caccgcggtc gtcgagttgg ccgacgcgtg
    14461 cgggatcgtg cacctccccg gtggggtcaa agctccgctg accgcgacgt cgcggggctt
    14521 gggacaggtc ttggcggcgg cgctcgatga cccgtcggtg acgtcggtgg tcatggggat
    14581 cggcgggagc gccagcaacg acggcggcgc gggaatgctt gcggcactag gggctcggct
    14641 gcttgacgct ggcggtcgtg agctgccgga cgggggtggg gcactggtcg atctggctgc
    14701 gctggatctg tctgggctgc acctgcggct gcgcgaggtg tcgctgacgg tggcgtgcga
    14761 tgtcaacaac ccgctgctcg gggagccagg ggcggtggcg atcttcggcc cgcagaaggg
    14821 ggcgggcgag gccgagcggg tggtgttgga ggcgggtctg gcgcggttcg cttcgttggt
    14881 caccgaagcg atgaggcggg acgacacggg cctgccaggt gcgggagccg ccggaggcgt
    14941 cgggtatgcc gcgcaggccg tgttgggtgc ggcgatgcgg cccggggtcg agttggtgtt
    15001 ggagttgatg gggttcgagg cgttgtgtga gggggcttcg ctggtggtga cgggtgaggg
    15061 gtcgctggac cggcagacgt tgctgggcaa gacgccggcg ggggtggcgc gcgcggccca
    15121 ggcgcacggc ataccggtgg tggcggtgtg tgggcggtcg ttgctgtcgg ccgaggacgt
    15181 ggccgcgtcg gggatcgcac aggtctatgc gctgaccgat cttgagcctg accccgccgt
    15241 ctgcatgtcc gacgccgccc gcctcctgcg cgcactgagc gcccaaatgg cccacgactg
    15301 gctcagttga tggctggtcg cctctcgaca ccgggccacc ttggctggaa tgcgt
//
LOCUS       DAOMZY010000034        14963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_3620_length_14963_cov_5.794875, whole genome shotgun sequence.
ACCESSION   DAOMZY010000034 DAOMZY010000000
VERSION     DAOMZY010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 14963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14963
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3620_length_14963_cov_5.794875"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PLK64_03345"
     CDS             complement(<1..549)
                     /locus_tag="PLK64_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV78806.1"
                     /translation="MVIRSRPATARTAKTRPAYAIDSVDHALRLAAMLQLEGALTVSD
                     AADRLGVARSTAHRVLAMLVYRDFAVQGSDRRYHAGPVLELATHSRSLTARLRTVALP
                     HLEGLVAVLGESANLIIRTGDRARFIASAESTQALRVEQREGMVFPAHLVSGGLVLLA
                     EAGEAEVTALYSGGVAGGVAGGV"
     gene            711..1859
                     /locus_tag="PLK64_03350"
     CDS             711..1859
                     /locus_tag="PLK64_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011691898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPV78807.1"
                     /translation="MNQPSVISPVHLSMGSSNTEPEPSAELADLYRGFEKELLVPLWT
                     EIGDLMPHTPKSKAQPHLWEWAKLLELAGLAGDLVPVGRGGERRAIALANPSLGGRPY
                     ATPTLWAAIQYLMPGEDAPEHRHTQNAFRFVVEGTGVWTVVERDPIPMNRGDFLPQFA
                     WNWHAHHNAADRPMAWIDGLDIPFQHYVESTFFDFGRDSISQAEHATPDRSRSQRLWG
                     HPGIAPLSQLGPTPGSPLLCYRWVDTDAALTDQLELEREGYAATIEPGHAAIRFLNPS
                     NAGDVTQTIRTQFHRITAGTETAPRRQTGSSVYQVFDGSGRVSVGDFSWTVGRGDMFV
                     VPSWFPLSIKSEASASDSDSGALDLFEFGDAPIFEKLNLARTLVEDNR"
     gene            1856..2701
                     /locus_tag="PLK64_03355"
     CDS             1856..2701
                     /locus_tag="PLK64_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPV78808.1"
                     /translation="MKLATIRLADGKTAAVRVDGDTLVHLGATDVGSLLAQPDWRSRA
                     AAVAGTTSYAAADATFATLVPTPSKVVCVGLNYKTHIQEMGRDLPEYPTLFAKFADCL
                     IGATDDILRPVETTEFDWEVELAVVIGSTVRRATTEQAEAAIAGFTVLNDITCRDWQF
                     RTREWLQGKNWESSTPVGPYLVTPDELPGGVRPTLAVRCEVDGEVMQSDNTGDLLFDP
                     VALVEYISTMVRLNPGDIIATGTPGGVGHARKPSRYLAGGEVVVTEIEGLGRLVNRVV
                     SDGGA"
     gene            2701..3441
                     /locus_tag="PLK64_03360"
     CDS             2701..3441
                     /locus_tag="PLK64_03360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HPV78809.1"
                     /translation="MGRTLDDARRWASFGTQVCRDAILTLDEDTFAEPSLLPGWTRKH
                     LVAHLAANAEAVRNLATWAATGVPTPMYSSLEQRAADIEAGSHAAGADLAVWFASTAT
                     ALAKAWDGLSDGAWQAPVVTAQGRTVPASETPWMRTREVMVHAVDLGTGVTFADLPAD
                     FLEALCDDIVAKRATAPIAGSPDIDICPTDLEGLSWKLPGSEATAPITVTGPLAACTA
                     YLAGRGDSHLSASRDDHTTPIPSLAAWL"
     gene            3476..4756
                     /locus_tag="PLK64_03365"
     CDS             3476..4756
                     /locus_tag="PLK64_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPV78810.1"
                     /translation="MSETQRPDVIVVGGGIGGLSAALALTRKGLTVRLLERAKEFGEV
                     GAGLQIAPNCTRMLADYGLLDEAKTLGVLPQAMVMKDATDGSELTRLDLVDLERTYGF
                     PYMVIHRSDLHGIFLRACQRAGVELLTDQQCVGYENTPTGASATFADGHVEEAEVVIA
                     ADGMHSVARGLLVDDQPVNSSYVAYRGAVTIDKVMENQVSTTDVVVYIGPGCHFVQYP
                     LRGGEMFNQVAVFQSPKALAGQEDWGTPDELDKAFEGMCEPIQLGLPLMWRDRWWRMF
                     DRNPILNWVTGKIALLGDAAHPPLQYMAQGAIMAIEDGWVLAEHVGAQQAQRTADGGT
                     GVDWDGALAAYNAVRPEHCRRVLSTGRVWGELWHVDGVKREQRNIILRGRDTYDYTFT
                     DWIYGPTALTPEQEAPLYPIIPLDSVKLPEGAEV"
     gene            complement(4783..5028)
                     /locus_tag="PLK64_03370"
     CDS             complement(4783..5028)
                     /locus_tag="PLK64_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78811.1"
                     /translation="MTTSSKTACQSCGMPIESGPYCQHCVDETGALQDFETRFERMVG
                     WQQRRDPSASREALESATLDYLATMPAWREHPRVTAR"
     gene            complement(5025..5489)
                     /locus_tag="PLK64_03375"
     CDS             complement(5025..5489)
                     /locus_tag="PLK64_03375"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HPV78812.1"
                     /translation="MVTEPDAEVRRFCELFPAAYLAFHRRDGKRNQLSGASRAVLLHL
                     AQTGPLTVGEAAVHLDRAQSVVSEIVNQLEGHGYVERENDPDDRRRTFIWLTPTGRQR
                     LERDAQVLDPDLVGRAFIGLTTQQRQDLIEALAALVASARGIPNPSERERDS"
     gene            complement(5555..6475)
                     /locus_tag="PLK64_03380"
     CDS             complement(5555..6475)
                     /locus_tag="PLK64_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HPV78813.1"
                     /translation="MATERLTVVRIPGNGAEDVLIGPKGHVWTATEDGSVFRLTPDGH
                     LVERVATTGGRPLGLELLGDGRILVCDGDRGLLAVDPGSGSVEVLTSHVDGQPLRFCN
                     NAAVHTDGRIFFTDTSSVYSVHQWKNDLVEATRSGRLLVRHTDGSVDTVLSGLEFANG
                     VALSADESYVAVAETGARTVVRQWLTGRHAGSHDYLAEDLPGYPDNMSRGSDGLIWVA
                     IASPRDPVVERLKAGPMPLRRGASKLPLWAQPKPKRTARVMAFDDDGEVVHDRTFDAT
                     HFHMATGVREHHGRVWLGSLQEPAIAWFDV"
     gene            6616..>6946
                     /locus_tag="PLK64_03385"
     CDS             6616..>6946
                     /locus_tag="PLK64_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78814.1"
                     /translation="MNVKKISIAAASVAAVAALAIGGSAIANAADNPTPTTTSSATST
                     QGTADSGTTGTQGKGQRGGTGATGQQGSQDTPVTGDEAAKVSAAVTAKDSTVTVTSVR
                     KDPDGSYD"
     assembly_gap    6947..7046
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7047..7447
                     /locus_tag="PLK64_03390"
     CDS             <7047..7447
                     /locus_tag="PLK64_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78815.1"
                     /translation="LGTKAGANVMFDVSADLATITENAGGPGGGKGGGMGGASQDTPV
                     TGDEAAKVSAAVTAKDSTVTVTSVRKDPDGSYDVLGTKAGANVMFDVSADLATITENA
                     GGPGGMGGGKHGAPGAADSTQTASSATSSS"
     gene            complement(7550..7870)
                     /locus_tag="PLK64_03395"
     CDS             complement(7550..7870)
                     /locus_tag="PLK64_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HPV78816.1"
                     /translation="MTRPERGEVWWCEPPDIGRRPVVVLSRSAAIIGRGRALVAPCTT
                     TVRGLPSEVALDPRTDPVPRRCVATLDAVESASLGVLVERLGRLSDTRMREMCAALAV
                     AVDC"
     gene            complement(7867..8106)
                     /locus_tag="PLK64_03400"
     CDS             complement(7867..8106)
                     /locus_tag="PLK64_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin MazE5"
                     /protein_id="HPV78817.1"
                     /translation="MAKIRVSTTVDATLLAQVRALDPTGTDASLLERAFTALLASQRR
                     AEVDAAYREAYAAHPADEPDAWGDLATFGAAVARS"
     gene            8194..8706
                     /locus_tag="PLK64_03405"
     CDS             8194..8706
                     /locus_tag="PLK64_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPV78818.1"
                     /translation="MAHTLVVIRHAKSDWDVDAADRDRPLAKRGRRQAPPTGRWLAAQ
                     DLALDLAVVSPAARARQTWELVAAQLDRPPTTRVEEAAYTFDGDELLEVVRALPESAG
                     AVVLVGHNPAVEEFVETLTGEYAALPTSAVAVVEVASWAGVGPGTGRLLANLRPADAE
                     LPDTELPGTD"
     gene            complement(8731..9564)
                     /locus_tag="PLK64_03410"
     CDS             complement(8731..9564)
                     /locus_tag="PLK64_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018776105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPV78819.1"
                     /translation="MTAAGRGQRHVFDTMGTTVSLVFAGSAPAGALDAVEAVFAALNR
                     RFSRYRDDSEASAVARGDLAVADSSPQFREAFALAEQWRADTLGAFTPYRPDGSIDLA
                     GVIKAQAILDAGDALRRHGATAWCLNAGGDVLTSGAQADGRPWVVGIVDPTDRTALIS
                     QFTCSAVHPAVATSGVAERGEHIWRAGDVASTSSVTAAPSSPTSPAWLPGSTETAEDR
                     LVQVTVAARDIMTADVLATAILAGGRRTLEHALVRWDIEVLACSAAGEFLATEAFRAA
                     D"
     gene            complement(9568..9792)
                     /locus_tag="PLK64_03415"
     CDS             complement(9568..9792)
                     /locus_tag="PLK64_03415"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HPV78820.1"
                     /translation="MTVTVTLVNGKITDITYKTTVGDNRSAQIEARSIPVLKAAVLAA
                     NSTDVSTVSGATYTTKKYLSSLQSALDQAA"
     gene            9851..10207
                     /locus_tag="PLK64_03420"
     CDS             9851..10207
                     /locus_tag="PLK64_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78821.1"
                     /translation="MPELVVPELVVPDVVPELVVPDVVPELVVPELVVPDVVPEPVVP
                     ELVVPEVVLVVVPAAVVDETVFPLAVPTFQPTRRIPAEARAAAVLLRVLIVVPSKRVE
                     RLALTTTLTTQAVREL"
     gene            10325..11773
                     /locus_tag="PLK64_03425"
     CDS             10325..11773
                     /locus_tag="PLK64_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018779920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase family protein"
                     /protein_id="HPV78822.1"
                     /translation="MTTHTFDAPQLDAPRGDLDQKPGARHTRSALLRRYQGQATRREV
                     RRDVLTVGAWSLVATSIALFLADNGIRYFATLGGAITALGIIAGLVATAFMCLMLVLT
                     ARVPVIDATLGQDRATSLHAKLGRGMVFGLLAHGILLVYGYALSDKITPIATFTQLWS
                     SSTDFIWACVAFLMLGVVGISSMVALRRKYPYEVWFGIHLTTYAAILASIPHQFSMSG
                     MLAEGTWGRPFWAAMWLVTGGLMLAYRVILPILASLTHQFTVSRVTPVGPDAVSIEVT
                     GRKVKELQARGGHWLNWRFLTPELAGQPHPFSLSAEPTDSTLRITVRNLGEGTARLMS
                     LKPGTKVAIEGPYGMFTDASRTTDKVVLLGAGIGIAPIRALLESTEFAPGDAAVILRA
                     SNPDELYLLDEVVALCEAKGASLALLVGPRAGQSWVPVTDAGATLIDFVPWAAEADVY
                     VCGPNPWMEAVLADVAACGIPAAQVHDEKFDW"
     gene            11867..12832
                     /locus_tag="PLK64_03430"
     CDS             11867..12832
                     /locus_tag="PLK64_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HPV78823.1"
                     /translation="MTEATTAQVQVLRGRLVPLGGIRAMYVRRTLPHRSRAFIGAWCF
                     VDHYGPNEIGADGSGGMDIPPHPHTGLQTVSWLFEGEIEHRDSGGAVGLVRPGEVNLM
                     TAGAGIAHTEVSTPETRRLHGVQLWVALPDAARDTHRNFEHHAPPLVPLRGVGSARVF
                     IEELAGVDRSPIHTFTPLLGAELILNPGAAVALAVDTAYEHGVLLDTGEVSVEGVPIE
                     SGDLACIDPGPDRLRLRAGAAGARIVLLGGVPFEEEIVMWWNFVGRSHDEILAYRQGW
                     EAGSIRFGTVKGYAGPVDRIPAPPLPAVRLRPRGRRGAGAEGNSS"
     gene            complement(12827..13264)
                     /locus_tag="PLK64_03435"
     CDS             complement(12827..13264)
                     /locus_tag="PLK64_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPV78824.1"
                     /translation="MTIHYVDASAWAKLLVEEPESGPLAAYVDERLAAGETFASSHLL
                     VTELHRLAARVGAAPTSVTAALSVLALTLPDAATFRAAGLLAGQVRSLDALHVASALD
                     LGVVDFVSYDQRQLAAAATAGIPAASPGAVPAASPAAHDGRAL"
     gene            complement(13261..13521)
                     /locus_tag="PLK64_03440"
     CDS             complement(13261..13521)
                     /locus_tag="PLK64_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009358999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HPV78825.1"
                     /translation="MTTLAHRDLRNNSAEILRRAAAGESFEVTNNGRVVAMLVPPQVS
                     PLERLFLARLVTPPVARRLPHIARVQTTRSTAEILDDLKGER"
     gene            complement(13615..13974)
                     /locus_tag="PLK64_03445"
     CDS             complement(13615..13974)
                     /locus_tag="PLK64_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018774355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HPV78826.1"
                     /translation="MTADPQTVEGYFDALPEATRTALQEVRSTLHDAIPGAQEKISYG
                     IPTLTLRGRNVVSFAGWAKHLSIYPIPAAPEGSELAQRIEAHRSGRGTLKFPLSEPLP
                     LDLIRAVGVLFADQTAG"
     gene            complement(14087..>14963)
                     /locus_tag="PLK64_03450"
     CDS             complement(14087..>14963)
                     /locus_tag="PLK64_03450"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPV78827.1"
                     /translation="GERCHFCFDTTQLRRIWVAAFGAALGPDAKLARALSRASGRPRK
                     DALGWHTGLRLTSAERLGELLRDAADTGGGLALHAVGNGAVAGVLDALAAERTRSGGS
                     AGSGVDTGAGANVRIEHALTLDEELARRLGATGLPVVTQPGFLRTHARQLVTIPPPAP
                     LMLFPLRSLLSHGAQLAFGSDYPAADLSPWPAIAAAVTRASGIPGRTVHPEQALTLTE
                     ALDATTATAARVLGVTDTGVLAAGLRADLQWVDADPFAVAPEALADIRARAVWHDGVQ
                     LVGDLASGISGPADT"
BASE COUNT         2242 a         5342 c         5078 g         2201 t
ORIGIN      
        1 aaccccaccg gcgaccccac cggcaacccc accggaatac agcgcggtca cctcggcctc
       61 gcccgcctcg gcgaggagca ccaacccgcc ggagaccagg tgcgcgggga aaaccatgcc
      121 ctcgcgctgc tccacccgaa gcgcctgagt tgactcggcg gaggcgatga agcgcgcccg
      181 gtcgcccgtg cggatgatga ggttagccga ctcgccgagc accgcgacga gcccctccag
      241 gtgcggcagg gcgaccgtgc gcagccgagc ggtcaacgag cgcgagtgag tcgcgagttc
      301 gagcaccggc cccgcgtgat agcgccggtc cgagccctgc acggcgaagt cgcgatagac
      361 gagcatcgcg agcacccggt gggcagtcga gcgcgcgact ccgagccgat cggccgcgtc
      421 gctcaccgtc agcgcgcctt cgagttggag catcgccgcg agccgcagcg cgtggtcgac
      481 gctgtcgatg gcgtatgccg gtcgcgtctt ggccgtgcgc gcggttgccg gccggctcct
      541 gatcaccacg ggaacaccac gggtttcacc acggcgcgag tctcccctcc ggaactgttg
      601 ctccggtcac cattctgtca gacagaacaa cctattcccc agggcgcaac cgggcgacga
      661 tgtgccgacg ccatcgcggg caccatcaga gaggatccaa ggagccgctc atgaaccagc
      721 catcggtcat ctcccctgtc cacctgagca tgggcagcag caacaccgag ccggagccgt
      781 cggccgaact cgccgacctc taccggggtt tcgagaagga actgctcgtc ccgctctgga
      841 ccgagatcgg cgacctcatg ccgcacacgc ccaagtccaa ggcgcagccg cacctgtggg
      901 agtgggccaa gctgctcgag ctcgcgggcc tcgccggcga cctcgtcccg gtcggccgcg
      961 gcggtgagcg ccgggcgatc gcgctggcca acccgagcct cggcggccgc ccctacgcca
     1021 ccccgacgct gtgggcggcg atccaatacc tcatgcccgg cgaggacgcc cccgagcacc
     1081 gccacaccca gaacgccttc cgcttcgtcg tcgagggcac cggcgtgtgg acggtcgtcg
     1141 agcgcgaccc gatcccgatg aaccgcggcg acttcctccc gcagtttgcc tggaactggc
     1201 acgcccacca caacgccgcc gaccgcccga tggcatggat cgacgggctg gacatcccct
     1261 tccagcacta cgtcgagagc acgttcttcg acttcggccg cgactcgatc agccaggctg
     1321 agcacgccac ccccgatcgc tcacgctcgc aacggctctg gggtcaccca ggcatcgcgc
     1381 cgctctcgca gctcggcccg acccccggct caccgctgct gtgctaccgc tgggtcgaca
     1441 ccgacgccgc cctcaccgac cagctcgagc tggagcgcga ggggtatgcc gcgacgatcg
     1501 agccggggca cgccgccatc cgcttcctca accccagcaa tgccggggac gtcacgcaga
     1561 cgatccgcac ccagttccac cggatcaccg ccggcaccga gaccgccccg cgccgccaga
     1621 ccggctcctc ggtctatcag gtgttcgacg gctccggccg ggtctcggtc ggtgacttct
     1681 cctggaccgt cggccgcggc gacatgttcg tcgtgccctc gtggttcccg ctgtcgatca
     1741 agtccgaagc ctcggcctcc gactccgact ccggggcgct agacctgttc gagttcggtg
     1801 acgcccccat cttcgaaaag ctcaacctcg cccgcaccct cgtggaggac aaccgatgaa
     1861 gctcgccacc atccgcctgg ccgacggcaa gaccgctgcg gtccgcgtcg acggagacac
     1921 cctcgttcac ctcggggcga cagacgtggg ttcgcttctg gcccagccgg attggcgctc
     1981 acgtgccgcc gcggtcgcag gcaccacgtc gtatgccgcc gctgacgcca ccttcgcgac
     2041 gctcgtcccg acgccgtcca aggtggtgtg cgtgggcctc aactacaaga cccacatcca
     2101 ggagatgggc cgggatcttc ccgaataccc cacgctcttc gcgaagttcg ccgactgcct
     2161 catcggggcg accgacgaca tcctccgccc ggtcgagacg accgagttcg actgggaggt
     2221 cgagctggcc gtcgtcatcg gcagcacggt ccggcgcgca acgaccgagc aggccgaagc
     2281 cgcgatcgcc ggcttcacgg tgctcaacga catcacctgc cgtgactggc agttccgcac
     2341 ccgcgagtgg ctgcagggca agaactggga gtcctcgacc cccgtcggcc cctacctcgt
     2401 cacccccgac gagctgcccg gcggcgtccg cccgacgctc gccgtccggt gcgaggtcga
     2461 cggcgaggtc atgcagtccg acaacacggg cgacctgctc ttcgacccgg ttgccctcgt
     2521 cgaatacatc tccacgatgg tccggctcaa ccccggcgac atcatcgcca ccggcacccc
     2581 cggcggggtc ggccacgccc gcaagccctc gcgttacctg gccggcggcg aggtcgtcgt
     2641 caccgagatc gagggcttgg gccggctggt caaccgcgtg gtcagtgacg gtggtgcctg
     2701 atgggccgca cgctcgacga cgcccgccgc tgggcctcct tcggcactca ggtatgccgc
     2761 gacgcgatcc tcacgctcga cgaggacacc ttcgccgagc ccagcctgtt gccgggctgg
     2821 acccgtaagc acctcgtcgc gcacctcgcc gccaacgctg aggccgtccg caacctcgcg
     2881 acgtgggcgg cgaccggcgt cccgaccccg atgtattcct cgctcgagca gcgggccgcc
     2941 gacatcgagg ccggcagcca cgccgccggc gccgacctgg cggtctggtt cgccagcacg
     3001 gccaccgcgc tcgccaaggc gtgggacggg ctgtccgacg gcgcctggca ggcaccggtc
     3061 gtcaccgccc agggccgcac ggtccccgcg tccgaaaccc cgtggatgcg cacccgcgag
     3121 gtcatggtcc acgccgtcga tctcggcacc ggcgtcacct tcgccgacct gccggccgac
     3181 ttcctcgagg cactgtgcga cgacatcgtc gccaagcggg ccacggcacc gatcgccggt
     3241 tcgcccgaca tcgacatctg cccgaccgac ctcgagggcc tcagttggaa gctgccgggt
     3301 tccgaggcga ccgccccaat caccgtcacc ggccccctgg ctgcgtgcac ggcatacctc
     3361 gccggccgtg gcgactcgca cctgagcgcg agccgcgacg accacaccac ccccatcccc
     3421 agcctggccg cctggctttg acggcggagc acacggatcg acacaggagg tcatcatgag
     3481 tgagacccaa cgccctgacg tcatcgtcgt cggcggcggc atcggcgggc tgagcgcggc
     3541 tctcgcgctg actcgcaagg gcttgacggt tcggctgctc gagcgggcca aagagttcgg
     3601 cgaggtgggc gccggcctgc agatcgcccc caactgcacg cgcatgctcg ccgactacgg
     3661 gctgctcgac gaggccaaga ccctcggcgt gttgccgcag gcgatggtca tgaaggacgc
     3721 caccgacggg tccgagttga cccggctgga cctggtcgac cttgagcgca cgtacggctt
     3781 cccctacatg gtcatccacc gcagcgacct gcatgggatc ttcctgcgcg cctgccagcg
     3841 ggccggcgtc gagctgctca ccgaccagca atgcgtcggc tacgagaaca ccccgaccgg
     3901 cgcgtcggcc accttcgccg acggacacgt cgaggaggcc gaggtggtca tcgccgccga
     3961 cggcatgcac tcggtcgccc gcggcctgct cgtcgacgac cagccggtca actcgtccta
     4021 cgtcgcctac cgcggtgccg tgacgatcga caaggttatg gagaaccagg tctcgacgac
     4081 cgacgtcgtc gtctacatcg ggccgggctg tcacttcgtg cagtaccccc tgcgtggcgg
     4141 cgagatgttc aaccaggtcg ccgtcttcca gtcgcccaag gcacttgcgg gacaagagga
     4201 ttggggcacg ccggacgagc tcgacaaggc cttcgagggc atgtgcgagc cgatccagct
     4261 cggcctgccg ctcatgtggc gcgaccgctg gtggcggatg ttcgaccgca acccgatcct
     4321 caactgggtc accggcaaga tcgccctgct cggcgacgcc gcgcacccgc cgctgcaata
     4381 catggcgcag ggcgcgatca tggcgatcga agacgggtgg gtgctcgccg agcacgtcgg
     4441 agctcagcag gcgcagcgga ccgctgacgg cgggaccggc gtcgactggg acggcgcgct
     4501 cgcggcatac aacgcggtcc gtcccgagca ctgccggcgg gtgctgtcca ccggccgggt
     4561 gtggggcgag ctgtggcacg tggacggcgt caagcgcgag cagcgcaaca tcatcctgcg
     4621 tgggcgcgac acctacgact acaccttcac cgactggatc tatgggccga cggcgctcac
     4681 gcccgagcag gaggcgccgc tctacccgat catcccgctc gactcggtca agctcccgga
     4741 gggcgccgag gtctgacctg ccgtctggcg gcggtgccgg gcttagcggg cggtcacccg
     4801 agggtgctcg cgccaggccg gcatcgtcgc aaggtaatcc agcgtcgccg actcgagcgc
     4861 ctcacgtgag gcgctcgggt cgcggcgctg ttgccatccc accatccgct cgaaccgggt
     4921 ctcgaagtcc tgcaacgccc cggtctcgtc aacgcagtgc tggcagtagg gccccgactc
     4981 gatgggcatg ccgcagctct ggcaggcagt cttgctggaa gtggtcatga atctctttcc
     5041 ctctctgatg gattcggtat gccgcgcgct gaagcgacca gggcggccag ggcctcgatc
     5101 aggtcctgtc gttgttgcgt ggtcaggccg atgaacgccc ggccgaccag gtccgggtcg
     5161 agcacctggg cgtcgcgctc caggcgctga cgcccggtcg gcgtcagcca gatgaacgtc
     5221 cggcggcggt cgtcggggtc gttctcccgc tcgacgtagc cgtgtccctc gagttggttg
     5281 acgatctcgc tgacgacgga ctgggcccgg tcgaggtgga ccgccgcttc tccaacggtc
     5341 agtggcccgg tctgggccag gtgcaggagc accgcccggg acgccccact cagttggttg
     5401 cgcttgccgt cccgccggtg gaaggcgagg tatgccgcgg ggaacaactc gcagaaccgc
     5461 cgcacctcgg cgtcgggctc ggtgaccatg cgcccactgt ggagggcacg gcatacatcg
     5521 tcaatcccga tggaatgtga gcggagtggc agggtcagac gtcgaaccag gcgatggcag
     5581 gctcttggag gctgcccagc cagacccggc cgtgatgctc gcgcactccg gtagccatgt
     5641 ggaagtgcgt cgcgtcgaag gtgcggtcgt ggacgacttc gccgtcatcg tcaaacgcca
     5701 tcacccgggc ggtgcgcttg ggtttgggct gggcccacag cggcagtttg gacgccccgc
     5761 gccgcagcgg catcggcccg gccttgagcc gctcaaccac cgggtcgcga ggcgacgcga
     5821 tggccaccca gatgagcccg tcactgccgc gggacatgtt gtcgggatag cccggcaggt
     5881 cttcggcgag gtagtcgtgg ctccccgcgt gccggccggt cagccactgc cgcacgacgg
     5941 ttcgcgcccc cgtctcggcc accgcgacgt aggactcgtc cgcggacagc gctacgccgt
     6001 tggcgaattc gaggccggac agcacggtgt caaccgaccc gtcggtatgc cgcacgagca
     6061 agcgaccgct gcgggtcgcc tcgacgaggt cgttcttcca ctggtggacc gagtagacgc
     6121 tcgacgtgtc ggtgaagaag atccgcccat cggtgtggac tgccgcgttg ttgcagaacc
     6181 tcagcggttg cccgtcgacg tgtgaggtca gcacctcgac actccctgac cccgggtcga
     6241 ccgcgagcag ccctcggtcg ccatcgcaga cgaggatccg gccgtcgccg agcaactcca
     6301 gccccagggg ccgaccaccg gtcgtcgcga cgcgctcgac gaggtgtccg tcgggcgtca
     6361 gccggaaaac cgagccatcc tcggtggccg tccagacgtg gcctttcggc ccgatgagca
     6421 cgtcctcggc gccgttcccg ggaattcgca cgacggtgag ccgctcggtg gccatgcgcc
     6481 cgagcctagc ggggagcggc gcgagcaggt cgtagccaac ccccacctgc gtgacaggtt
     6541 ggtcataggt gggcacccga gtcttctcat cagcagaggt cgtcaagagg cctcgacccg
     6601 acaaggagaa gcccgatgaa cgtcaagaag atcagcatcg cagccgcatc agtcgccgcc
     6661 gtcgccgcct tggcgatcgg tgggtccgcg atcgcgaacg ccgccgacaa cccgaccccg
     6721 acgaccacct cctcggccac gtccacgcag ggcaccgccg actccggcac cacgggcacc
     6781 cagggcaagg gccagcgcgg cggcaccggt gccaccggtc agcagggctc gcaggacacc
     6841 ccggtgaccg gtgacgaggc cgcgaaggtc tcggctgcgg tcactgccaa ggactcgacc
     6901 gtcacggtga cctcggtccg taaggacccc gacggttcct acgacgnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnntgct cggcaccaag gccggcgcca acgtgatgtt
     7081 cgacgtcagc gccgacctgg ccacgatcac cgagaacgcc ggcggccccg gcggtggcaa
     7141 gggtggcggc atgggcggag cttcgcagga caccccggtg acgggtgacg aggccgcgaa
     7201 ggtctcggcc gcggtgaccg ccaaggactc gacggtcacg gtgacctcgg tccgtaagga
     7261 ccccgacggt tcctacgacg tgctcggcac caaggccggc gccaacgtga tgttcgacgt
     7321 cagcgccgac ctggcgacga tcaccgagaa cgccggtggc cccggcggca tgggcggcgg
     7381 caagcacggc gcccccggcg cggccgactc cacccagacc gcttcgagcg ccacctcctc
     7441 cagctgaccc gacccgcgcg cggactcccg cacccgcgcg cggcataccc gcaaggcccg
     7501 tgacccaccc cctggggtca cgggcctttg cgtatgccgc ctcgtcccgt tagcagtcga
     7561 cggccaccgc gagcgccgcg cacatctcgc gcatccgggt gtcggacaat cggccgagcc
     7621 gctcgacgag caccccgagg gatgccgact cgacggcgtc gagggtggcg acacagcggc
     7681 ggggcactgg gtcggttcgc gggtcgagcg ccacttcgct gggcaagccg cgcacggtcg
     7741 tcgtgcaggg agcgacgagc gctcgccccc gcccgatgat cgccgcggag cgggagagaa
     7801 cgacgaccgg gcgccgcccg atgtccggtg gctcgcacca ccacacctcg ccgcgctccg
     7861 gccgagtcat gagcgggcca ccgcggcgcc gaacgttgcc aggtcgcccc acgcatccgg
     7921 ctcatcggcg gggtgggcgg catacgcctc ccggtatgcc gcatcgacct cggcccggcg
     7981 ctggctcgcc aacaacgcgg tgaaggcccg ttccagaagg ctggcgtccg tgccggtcgg
     8041 atccagcgcc cggacctggg ccaacaacgt ggcgtccacg gtggtgctta cccggatttt
     8101 ggccatgcca caactatgcc acgcctacag cacgatcatc gcgtcgctct ggggttaggg
     8161 tgagccagcc acgtgcccac aaagggagga cccatggccc acacgctcgt cgtcatcagg
     8221 cacgcgaagt ccgactggga cgtcgatgcc gccgaccggg accggccgct ggccaagcgt
     8281 gggcgccggc aggctccgcc gactgggcgc tggttggccg cgcaggacct cgcgctcgac
     8341 ctggcggtcg tctcaccggc ggccagggct cgccagacct gggagctcgt cgcggcgcag
     8401 ctcgaccggc cgccgaccac gcgcgtggag gaggcggcat acacctttga cggcgacgag
     8461 ttgctcgagg tggtgcgggc gttgccggag tcggcgggcg cggtcgtcct cgtgggacac
     8521 aatccggcgg tcgaggagtt cgtggagacg ttgaccggtg agtatgccgc gctgcccacc
     8581 tccgcggtcg cggtcgtcga ggtggcgtcc tgggcgggcg tcgggccggg gactggtcgg
     8641 ctgctggcca acctgcgtcc cgccgacgcc gaactgcccg acaccgaact gcctggcacc
     8701 gactagctga tagccgggcg gcggcggggc tcaatcagcg gcgcggaagg cctcggtggc
     8761 gaggaactct cctgcggcag agcacgcgag cacctcgatg tcccagcgaa cgagggcgtg
     8821 ctcgagggtg cggcggccac cggcgaggat cgccgtcgcc aggacgtcgg cggtcatgat
     8881 gtcgcgcgcg gcaacggtga cctggacgag gcggtcctcg gcagtctcgg tcgagcccgg
     8941 cagccaggcc ggtgaggtcg gcgaggacgg tgctgcggtg accgatgacg ttgacgcgac
     9001 gtcaccggcg cgccagatgt gctccccgcg ctcggcgaca ccactggtgg cgaccgcagg
     9061 atgcactgcg ctgcaagtga attgggatat cagggcggtg cggtccgtcg ggtcgacgat
     9121 ccccacgacc cagggccggc cgtctgcttg ggcaccggag gtcaggacgt cacccccggc
     9181 gttgaggcac caggccgtgg cgccgtgtcg acgcaacgca tcgcccgcat ccaggatcgc
     9241 ttgggccttg atgactccgg cgaggtcgat cgagccgtcc gggcgatagg gagtgaaggc
     9301 ccccagggtg tcggcgcgcc actgctcggc gagtgcgaag gcctcccgga actgcggcga
     9361 agaatcggcg acggcgaggt cgccgcgggc cacggcgctg gcctccgagt cgtcgcggta
     9421 acggctgaac cgacgattga gggcggcgaa caccgcctcg acggcatcaa gcgcacccgc
     9481 gggggccgag ccggcgaaga ccaggctgac cgtcgtgccc atcgtgtcga agacgtggcg
     9541 ttggcctcgg cccgcagcgg tcatggctca ggcggcctgg tcgagtgcgg actgcagcga
     9601 ggagaggtac ttcttggtcg tgtaggtcgc gccgctcacc gtggagacgt cggtcgagtt
     9661 ggcggcgagg acggcggcct tgagaacggg gatcgagcgg gcctcgatct gggcgctgcg
     9721 gttgtcgccc accgtcgtct tgtaggtgat gtcggtgatc ttcccgttga ccagggtgac
     9781 ggtcaccgtg atggtgcccc accggtcgga cttggtggtc ccggtgtagg tgccggtcgc
     9841 tgtcgaggtc gtgccggagc tggtcgtccc ggaactcgtc gttcccgacg tcgttccgga
     9901 gctggtcgtt cccgacgtcg ttccggagct ggtcgttccc gagctggtcg tgcccgacgt
     9961 cgttccggag ccggtcgttc cggagctggt cgtgcccgag gtggtgctcg tggtggtgcc
    10021 tgcggccgtg gtggacgaga cggtctttcc gttggccgtg ccgacgttcc agccgaccag
    10081 gaggatcccg gctgaggcta gggcggccgc tgtcttgcta cgtgtgctca tcgtcgtgcc
    10141 ttccaagagg gtggagcgtc tggcgttaac gacgactctg acgacccagg ctgtgcgcga
    10201 gttgtgagca ccgaggggcc tggctgagaa tcccgattct tcttgcaagc gcttccaccg
    10261 agtcagatcg acatcaaagt cggctcacaa gaaccgcaca gacccgaccc ggaaggtaag
    10321 ggccatgacc acgcacacct tcgacgctcc ccagctcgac gcgccccgcg gggacctcga
    10381 ccagaagccg ggcgcgcggc atacccgatc tgctctcctg cgccgctacc aggggcaggc
    10441 cacccggcgc gaggtgcgcc gcgacgtcct caccgtgggc gcctggtcgc tcgtcgcgac
    10501 ctcgatcgcg ctgttcctcg ccgacaacgg gatccgctac ttcgccacgc tgggcggcgc
    10561 gatcaccgcg ctcggcatca tcgccggcct cgtcgcgacc gccttcatgt gcctcatgct
    10621 cgtgctcacc gcacgggtgc cggtcatcga cgcgaccctc gggcaggacc gcgcgacctc
    10681 gctgcacgcc aaactcggcc gcggcatggt cttcgggctc cttgcccacg gaatcctgct
    10741 cgtctacggc tacgcgctgt cggacaagat cacgccgatc gcgaccttca cccagttgtg
    10801 gtcctcctcg accgacttca tctgggcctg cgttgccttc ctcatgttgg gcgtggtcgg
    10861 catctcctcg atggtcgccc tgcgccggaa gtacccctac gaggtgtggt tcggcatcca
    10921 cctgaccacg tatgccgcga tcctcgcctc catcccgcac cagttctcga tgagcgggat
    10981 gctcgccgag ggcacctggg gccggccatt ctgggccgcg atgtggctgg tcactggcgg
    11041 cctgatgctc gcctatcgcg tcatcctgcc gatcctggcc agcctcaccc accagttcac
    11101 cgtcagccgc gtcaccccgg tcggcccgga cgccgtctcg atcgaggtca ctggacgcaa
    11161 ggtcaaggag ttgcaggcgc gcggagggca ctggctcaac tggcgcttcc tcacccctga
    11221 gctcgccggc caaccgcacc cgttctcgct gtcggccgag cccaccgact cgacgctgcg
    11281 gatcaccgtc cgcaacctcg gcgagggcac ggcccgcctc atgtcgctca agcccggcac
    11341 caaggtcgcc atcgaaggcc cctacgggat gttcaccgac gcctcccgga cgaccgacaa
    11401 ggtggtgctc ctcggcgccg gcatcgggat cgccccgatc cgcgcgctgc tcgagtcaac
    11461 cgagttcgcc cccggcgacg ccgcggtcat cctgcgagcg tccaacccgg acgagctcta
    11521 cctgctcgac gaggtcgtcg cactgtgcga agccaaggga gcctcgctcg cgctgctcgt
    11581 cggcccgcgc gctggtcagt cgtgggtgcc ggtgaccgac gccggcgcca cgctgatcga
    11641 cttcgtcccc tgggccgccg aggctgacgt ctacgtctgc ggacccaacc cgtggatgga
    11701 ggcggtgctc gccgatgtgg ccgcgtgcgg catacccgcc gcgcaggtgc acgacgagaa
    11761 gttcgactgg taaccgcacc agccgcgcag gcgagggggg cgatccggta tgccgggtcg
    11821 tcccctcagc cgtgcgcacg cccgggccgc gtgctctact ggcctcgtga ctgaggcgac
    11881 gactgcgcag gtccaggttc tccgcggtcg tttggtgccc ttgggcggca tccgggcgat
    11941 gtacgtgcgg cgcaccctcc cgcaccgctc ccgcgccttc atcggcgcct ggtgcttcgt
    12001 ggatcattac ggccccaacg agattggtgc cgacggtagt ggcgggatgg acatcccgcc
    12061 gcacccgcac accggcctgc agaccgtctc gtggctcttc gagggcgaga tcgagcaccg
    12121 cgactccggc ggcgcggtcg gcctcgtgcg ccccggcgag gtcaacctca tgacggccgg
    12181 cgccggcatc gcccacaccg aggtctcgac gccggagaca aggcggctgc acggcgtgca
    12241 actgtgggtc gccctgccgg acgcggcgcg cgacacccac cgcaacttcg agcaccacgc
    12301 gccgccgctc gtgccgctgc gcggtgtcgg gtcggcgcgt gtgttcatcg aggagttggc
    12361 cggtgtggat cgctcgccga tccacacctt cacgcccttg ctcggcgccg aactcatcct
    12421 caacccgggt gcggcggtcg cgctcgcggt cgacacggca tacgagcatg gcgtgctcct
    12481 ggacaccgga gaggtgagcg tcgagggcgt cccgatcgag tccggtgatc tcgcctgcat
    12541 cgaccccggc cccgaccggc tgcgactgcg ggcaggggcg gccggcgcgc ggatcgtgct
    12601 gctcggcggc gtgccgttcg aggaagagat cgtcatgtgg tggaacttcg tgggccgcag
    12661 ccacgacgag atcctcgcct atcggcaggg gtgggaggcg ggctcgatcc ggttcgggac
    12721 cgtcaagggg tatgccggtc ccgtcgatcg cattcccgcg ccaccgctgc ccgcggtgcg
    12781 gcttcgcccg cgtggtcgcc gaggcgctgg ggctgagggc aacagttcat agcgcccgcc
    12841 cgtcatgagc cgctgggctt gccgccggaa cagcccctgg gcttgccgcc ggaatccccg
    12901 ccgtggccgc ggccgcgagt tggcgctggt cgtagctcac gaagtcgacc actccgaggt
    12961 cgagcgccga ggcgacatgc agcgcgtcga ggctgcggac ctgacccgcc agcaatcccg
    13021 ccgcccggaa ggtcgcggca tccggcaggg tcagcgccag gaccgacagg gccgccgtca
    13081 cgctcgtcgg cgctgcgccg acccgggcgg ccagccggtg caactcggtg accaggaggt
    13141 gcgaggaggc gaaggtctcg cccgcggcca gccgctcgtc gacgtatgcc gcgagcggcc
    13201 cgctctcggg ctcctcgacg agcaacttgg cccaggccga cgcgtcgacg tagtggatcg
    13261 tcatcgctcg cccttgaggt cgtcgaggat ctcggccgtc gaccgggtgg tctggacgcg
    13321 cgcgatgtgg gggagccgtc gagcaacagg cggcgtgacg aggcgagcca ggaacaggcg
    13381 ctccaacggt gacacttggg gtgggacgag catcgccacc acccgcccgt tgttggtcac
    13441 ctcgaacgac tcgccagcgg ccgcacgacg cagaatctcg gcgctgttgt tgcgcaggtc
    13501 gcgatgcgcc aaagtggtca tacgctcact gtagcaatgt gctacgacaa ggggtcagtc
    13561 ccagcgggca ctcacggttg gtgtgtcaca accagccgga ttgctgagga caactcagcc
    13621 ggcggtctga tcggcgaaga gcaccccgac ggcacgaatg aggtcgagcg gcagcggctc
    13681 gctcagcggg aacttcaggg tgccgcgacc ggaccggtgg gcctcaatgc gttgcgccaa
    13741 ctcgctgccc tcgggcgcag cggggatcgg gtagatcgac aagtgcttgg cccagccggc
    13801 gaacgagacc acgttgcgac cgcgcagggt gagggtcggt atgccgtaac tgatcttctc
    13861 ctgcgccccg ggaatggcgt cgtgcagggt tgatcggacc tcttggagtg cggtgcgggt
    13921 tgcctctggg agggcgtcga aatatccctc gaccgtctgc gggtcagccg tcatcgcgcc
    13981 actgtacgac tggctgccct ctgttccccg atttcctcgc acccgccggt gttctcgcgc
    14041 gccggaggcc cgttgaggcc cgatttcctc acagccgtgc ggcttctcag gtgtccgccg
    14101 gccccgagat cccgcttgcg agatcgccga ccagttgcac accgtcgtgc cacaccgctc
    14161 gggcgcggat gtcggccaag gcctcaggcg cgacggcgaa cgggtccgcg tcgacccact
    14221 gcaggtcggc ccgcaggccc gccgcgagca cgcccgtgtc ggtcacgccg agcacgcggg
    14281 cggcggtggc cgtggtcgcg tcgagtgctt ccgtgagcgt caaggcctgc tcgggatgga
    14341 ccgtccgccc cggtatgccg ctcgcccggg tcaccgccgc agcgatcgcc ggccaggggg
    14401 agaggtcggc agccgggtag tcggagccga aagccagctg ggcgccatgg ctgagcaagg
    14461 accgcaaggg gaagagcatc agcggcgccg gcggcgggat cgtcaccaac tggcgggcgt
    14521 gcgtccgcag gaacccgggc tgggtgacga ccggcagccc cgtggcgccg aggcgtcgag
    14581 ccaactcctc gtcgagagtg agcgcgtgct cgatccggac gttggcgccg gcgccggtat
    14641 cgactccgga gccggcactg ccgccggagc gggtgcgctc cgccgccagc gcgtcgagga
    14701 ctccagcaac ggcgccgttg ccgacggcgt gcaacgccag cccgccgcca gtgtcggcag
    14761 cgtctctcag caactcaccg aggcgctccg ccgaggtcag ccgcaagccg gtgtgccatc
    14821 cgagggcgtc cttgcgaggc ctcccggaag cgcgcgacag ggcccgcgcg agcttggcgt
    14881 ccggccccag cgcagcgccg aacgcggcaa cccagatccg gcgcaattgc gttgtgtcaa
    14941 aacagaagtg gcaacgctca cca
//
LOCUS       DAOMZY010000035        14888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_3647_length_14888_cov_5.255107, whole genome shotgun sequence.
ACCESSION   DAOMZY010000035 DAOMZY010000000
VERSION     DAOMZY010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 14888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14888
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3647_length_14888_cov_5.255107"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PLK64_03455"
     CDS             <1..436
                     /locus_tag="PLK64_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar epimerase"
                     /protein_id="HPV78828.1"
                     /translation="DDLASGVRVVCEQDLGPVVTVGAPDWLSQEQVESLTGMRRVQLS
                     EATALGTAARLHRFGVLPAPASELAHALYPWVIDSARLRTVGWAAAYDNETCVGAMLE
                     SIKGRHAVAGRRVDRMDAALGAASAAVALVGTAAILRRARRP"
     gene            460..897
                     /locus_tag="PLK64_03460"
     CDS             460..897
                     /locus_tag="PLK64_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HPV78829.1"
                     /translation="MDAEVGGAVALARVQDQPLSVDEVLAAVRHDRAGAVALFVGQVR
                     DHDGGRGVVALDYSAHPSADSVAETITQRYAARPGIERVAMVHRVGSLVVGDLAIVAA
                     VSAAHRGEAFEACSQLVEEVKAALPIWKHQQFADGTHEWVGIG"
     gene            894..2042
                     /locus_tag="PLK64_03465"
     CDS             894..2042
                     /locus_tag="PLK64_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPV78830.1"
                     /translation="MSGSSSWSGSWTPPEETMPVVESRGARHTRRFWPPWAGLATGMF
                     LAMVLAVLASVIPLPYAILKPGPATDVLASHDGGDGQQTPRIAIDGAPTFATTGSLEF
                     TTVRVEGGPGFPVNAWDLLTAWLSPAQDVYHVDELFPPAATQEQVAEENKAEMAGSQQ
                     EAAAVALRALGQEVPQVVVVRQVVKGAPAEGELQAGDVIVSIGGAAARDSTAIRTAIQ
                     GVTPGDTVEVVVERSGAQVTTHPDTGKADDGRTVLGIVLGVDYKLPFTVKIDVGNVGG
                     PSAGLMFSLGIYDKLTDGALTGGSVIAGTGTIDEKGSVGPIGGIAQKMVGAREGGATF
                     FLAPADNCAEAAGREPSGLQVVKVSTFDDALAAVTAIGKGNTSGLPHC"
     gene            2136..5141
                     /locus_tag="PLK64_03470"
     CDS             2136..5141
                     /locus_tag="PLK64_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HPV78831.1"
                     /translation="MDEEKWFDGDTDQPPNTPPPGGRPVVAPRRSPLGVTVAVLIGLG
                     IVVTAVAQVWTEVMWFDSVNLRSVFTTQLGAQLLLGIGGGLITAALVWSSLHFAHRAR
                     PIYPPVTPQQDALDRYREALEPIRRLATLVIPLIAGLATGLTASSQWETFLLWRNAQP
                     FGITDPQFGLDLGFFVFSLPWLTFAVGFVTMAAVLAAIAAAFTHYVYGGLQLANRGRD
                     TTRFALLHLSSLFAVIVLLRAASYWLERYTLSLSTSALMTGIQYTDANAVLPTKAILA
                     VASLMCAAMFLSVIWTRSWRLPVVGTVLLLITSVVVGGIFPALIQSLKVKPSEKSLEA
                     PYIARNIEATRAAYGLGAIEVSTYVPSDEASAENLRATAAAIPGIRIVDPNVVSPTVR
                     QKHAVRNYYAFPDALDVDRYTIDGKLRDVVVAARELDLTGVPAGQRNWLNDHTVYTHG
                     YGIVAAYANQQDSEGNPVYVEAAIPAGTALGEYEPRIYFGEQSPDYAIVGAPDGAAPR
                     EFDYPAQVESQQVNNTYAGTGGVPLDNTIRKLAYGLKYRELNFMLSDAVNASSRIMDY
                     RTPKERVGRVAPWLTLDGNAYPAIVDGRVLWIVDGYTTTANYPNSGLTSMAASTRDST
                     TSTRRSVTSLEQGQLNYIRNSVKATVDAFDGSVHLYSWDDADPILAAWRSAFPGVVEP
                     RENISASLMSHLRYPEDLFKIQRTLLAKYHVQDPDSFYGGQDFWRIPQDPTQETARTD
                     QPPYYLSIAMPGQTDPNFSLTTTFMPTGNREVLTGFLAVDSNPGNQPEGKRLDGYGTL
                     RLLELPPNSSVKGPGQVQNDIASSNQSSKAFTLTLSQFINNARQQGSVVTLGNLLTLP
                     MGGGMLYVEPIYVQGTAASQFPLGRAIVVAFGNKLAWSNTLDGALNELFGGQVVPPTT
                     PTTPTTPTTPTTPTPGPTTPTTPAPGDLAQAIADAQKAYADGETALKAGDFAAYGDAQ
                     KRLKDALARAAAASPTGSLTVTPSPTASG"
     gene            5331..5404
                     /locus_tag="PLK64_03475"
     tRNA            5331..5404
                     /locus_tag="PLK64_03475"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5365..5367,aa:Met,seq:cat)
     gene            5556..5954
                     /locus_tag="PLK64_03480"
     CDS             5556..5954
                     /locus_tag="PLK64_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78832.1"
                     /translation="MAATTIAEIERGVFGKERSDPPQGAVLRRWFEDRVLTAFAGRVL
                     PFDLPAARLLATYRVPQHAPLDAALIAAIAQAAQMTVATRHARHFEARLHEASDACMP
                     LQPHASATKLIQAGRGGPGTSAAGGQLGGG"
     gene            complement(6018..6221)
                     /locus_tag="PLK64_03485"
     CDS             complement(6018..6221)
                     /locus_tag="PLK64_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPV78833.1"
                     /translation="MAQGTVKWFNAEKGFGFIAQDAGGPDVFVHYSAIDSGGYRSLDE
                     GQRVEFDVTQGQKGPQAEKVRGI"
     gene            complement(6423..7850)
                     /locus_tag="PLK64_03490"
     CDS             complement(6423..7850)
                     /locus_tag="PLK64_03490"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF005450.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HPV78834.1"
                     /translation="MTDPAMWDVATLSTAFGSGSLSPVEVLSAVRARIAAFEPTINAL
                     VRHDAATTAALDRESALAAAQSEDRWRHGEPLGPLDGIPVTVKENLARAGVAMQGGCA
                     GATPVVPVRDSPVVERLREAGAVIVGSTTMPDWGMLSSGVSSLHGITRSPWDPALTTG
                     GSSAGAGAAAVAAYGAIHVGTDIGGSIRLPGTWLGLVAHKPSFGRVPLDAPYLGRVAG
                     PLTRTTADAAVAMSVLARPDDRDWTALPAHEADWSVETASVRGLRVGLWLDAGAGMPC
                     DPAVRAVAESSGRVFESGGAVVEEIQPWLTPQMLADIDLFWRVRSLADLETLTPENRS
                     RVLPFVRRWAEGGARTSGLDVLRAYQSVMAMRAATVAATAAYDLIVSPVAPVAAFPAE
                     WPMPWGEADEGMAHIGFTVPFSMSGQPACSVNAGFLDDGWTVGVQVAGRRFDDVGVLR
                     AVGWFEGVRGPTATPRWPAQDAPQD"
     gene            8059..9597
                     /locus_tag="PLK64_03495"
     CDS             8059..9597
                     /locus_tag="PLK64_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPV78835.1"
                     /translation="MTHRLPRLIAPLAALALALTACTAGAGTAPAPTATGGSTAAGGP
                     STDKITLGLVAEPASLDFTKTDGAAIPQALLVNVYEGLVKLDQDGKIVPALAASWKVS
                     DDHKTYTFELTDKAKFSNGAAFTADDAVFSINRVKTDWTTSLKAAMDVVTEAKAISPT
                     QLQVTLSAPSNDWLYRMTTHIGAMFSKTGVEDLANKPVGTGPYTFTSWNRGDSITLTR
                     NDGYWGTKPYYQAVVLKYFKDATALNNALLTGTINVVTTVQAPEALGQFTSNAKYQVI
                     EGTTNGEVLLSFNNAQEIFKDKKVRQAVRYGIDHKALMDTCWAGKGTLIGSFVPPTDP
                     WYEDLTGVTPFDKAKATALLAEAGKTGTTLRLRVPTLPYATSCGQVVKAQLEAVGFKV
                     TLDSLEFPAAWLTTVFKGGDFDMSIVAHVEPRDLKGVFGNPEYYTHYGTADIQALLKA
                     ADEGSADDQVTNLKKAARLIAEDAAGDVLFLLPNLMVADKGITGLPKNGIAVAFDLSK
                     LAKA"
     gene            9618..9845
                     /locus_tag="PLK64_03500"
     CDS             9618..9845
                     /locus_tag="PLK64_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78836.1"
                     /translation="MPPAHLERAAYLCVSLASSTRSVISVARAHRMPMVGRWQQVRAW
                     RALSVVTNGIRYAARSLRGGRAAYFPSDRAT"
     gene            9858..10808
                     /locus_tag="PLK64_03505"
     CDS             9858..10808
                     /locus_tag="PLK64_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV78837.1"
                     /translation="MLLRIAERTAVLLVSLGVSSALVFGFMVLLPGDPARVALGVNAT
                     EADVSRLRLEFGLERPLVEQYLSWVGGLVHLDPGISYVSRAQIGPQIADRLQVSLILV
                     AASMTIAVALALPFGTLMAVHHRKASGLVLSALSQVGVAVPAFLAGILLITVFAVQLR
                     WLPASGWTPPAYDPGMFLQQLLLPALSLGLVQGAVLTRYVRSAVLDVLREDFLRTARA
                     KGLTPMRALVRHGLRNAAVPVVTVLGLQLATLLVGAVVIERVFVIPGLGSLLLDAVGN
                     RDLIVVQDVVMVLVVAVLIVNFVVDLLYLAIDPRLRTVAR"
     gene            10805..11680
                     /locus_tag="PLK64_03510"
     CDS             10805..11680
                     /locus_tag="PLK64_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV78838.1"
                     /translation="MSRLSRGAEAVMPPPGRRSRAGVEATLLVGGGIVAAITVLALVS
                     LVWTPYDVGEINATERLATSSARHWLGTDKFGRDVVSLLMVGSRTTLLVGLVSVGVAA
                     VIGVPLGILAAMVPKWWGEVLMRANDLLLAFPALLLAIMLTAVHGQGVVTAMIAIGIS
                     SIPSFARVVRSGAIGVMKTEYVVAARAAGRRPIAIALRHVLPNVGSLVIVQASVSFAI
                     AVLAEAALSYLGLGAPPGTPSWGRMLQEGQELLFVAPRLEVFPGVAIALAVLGFNLLG
                     DGLRDHFDPRLEDRR"
     gene            11719..13547
                     /locus_tag="PLK64_03515"
                     /pseudo
     CDS             11719..13547
                     /locus_tag="PLK64_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019159098.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            13706..14776
                     /locus_tag="PLK64_03520"
     CDS             13706..14776
                     /locus_tag="PLK64_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HPV78839.1"
                     /translation="MTENPAIRPGRTNSLTDVDGIRVGHATRIGDGWLTGTTVVLAPD
                     GGAVGGVDVRGGGPGTRETDLLDPRNMVDRVHAVVLSGGSAFGLAAADGVMVRLASAG
                     VGFPVGGPSEVVPIVPAAVIFDLGRGGSFGKRPDAAMGNDALVVANDGPVQQGCVGAG
                     TGARAGGLKGGVGSASAVLPDGTTVAALIVVNAVGSCVDPLTGELYAARFGLPGEFPS
                     LGSVSEADLAAARERAAAAGPGLGLGPGLATTIGVIATDATLTKAQCAKVSGIGHDGM
                     ARAIRPVHTMFDGDTLFTLASGARGAAGAPDPLAFHALLDAAGDCVTRAIGHAMLSAT
                     TVTPPAGEIRCLRDAFPSAFAH"
BASE COUNT         2201 a         5234 c         5102 g         2351 t
ORIGIN      
        1 cgatgacctg gccagcggcg ttcgggtcgt ctgcgagcaa gatctcgggc cggtcgtcac
       61 ggtcggcgct ccagactggt tgagccagga gcaggtcgag tcactcaccg ggatgcgccg
      121 ggtccagctg tccgaggcga ccgccctcgg cacggcggcg cgtctgcacc gcttcggggt
      181 gctgcccgcc ccggccagcg agttggcgca tgcgctctat ccgtgggtca tcgactcggc
      241 gcggctgcgg acggtcgggt gggccgcggc atacgacaat gagacctgcg tgggcgccat
      301 gctggagtcg atcaagggtc gtcatgcagt cgccggtcgc cgggtcgacc ggatggacgc
      361 ggcgttgggt gccgcgagcg cagccgtcgc tctggtgggc acggcggcaa tcctgcgacg
      421 ggctcgacgg ccatgacggc gaccgaggga ggtagcggcg tggacgctga ggtgggcggc
      481 gccgttgcgc tggctcgggt gcaggatcag ccgttgtcgg tcgacgaggt gctcgctgcc
      541 gtgcggcacg accgggcagg cgcggtcgcc ctcttcgtcg gccaagtgcg cgaccacgac
      601 ggtgggcggg gcgtcgtcgc gctggactat tcggcgcatc ccagcgctga ctcggtcgcc
      661 gaaaccatca cgcagcggta tgccgcccgc cccggtatcg agcgggtcgc gatggttcac
      721 cgggtcggat cgcttgtcgt cggagatctc gccatcgtcg ccgcagtgag cgcggcgcat
      781 cggggcgagg cgttcgaggc gtgctcccaa ctcgtcgaag aggtcaaggc agcgctcccg
      841 atctggaagc atcagcagtt cgcagacgga acgcacgaat gggtgggcat cggatgagcg
      901 gctcaagctc gtggtcaggg tcgtggaccc cgcccgagga gacgatgccc gtggtcgaga
      961 gccggggcgc acggcatacc cgacggttct ggccaccctg ggcgggtctg gccaccggga
     1021 tgttcctggc gatggtcctc gcggtgctgg cctcggtgat ccccctgccc tacgcgatcc
     1081 tcaaaccggg cccggccacc gatgtgctgg cctcccacga cggtggggac gggcagcaga
     1141 cgccgcgaat cgccatcgat ggtgccccga ccttcgcgac gaccgggtcg ttggagttca
     1201 cgacggtccg ggtcgagggc gggccgggct tcccggtcaa cgcgtgggac ctgttgacgg
     1261 cctggctgag cccagctcag gacgtctatc acgtcgatga gttgttcccg ccggcggcca
     1321 cgcaggagca ggtggccgag gagaacaagg ccgagatggc cggctcgcag caggaggcgg
     1381 cagcggtcgc cctccgagcg ctcggccaag aggtccctca ggtcgtcgtc gtgcgccagg
     1441 tcgtcaaggg ggcgccggcc gaaggagagc tgcaggccgg tgacgtcatc gtgtcgatcg
     1501 gcggggcggc cgcccgcgac tcgaccgcga tccggacggc gatccaaggg gtgacgcccg
     1561 gcgacaccgt cgaggtcgtc gtcgaacggt caggggcgca ggtgaccacc catcccgaca
     1621 ccggcaaggc cgacgacggc cggacggtgc tcggcatcgt gctgggcgtc gactacaaac
     1681 tgccgttcac ggtgaagatc gacgtcggaa acgtcggcgg gccgtcggcg gggctgatgt
     1741 tctcgttggg gatctacgac aagctcaccg acggggcgct gaccggtggt tcggtgatcg
     1801 ccgggaccgg caccatcgac gagaaggggt cggtcgggcc gatcggaggc atcgcccaga
     1861 agatggtggg agcccgtgag ggcggcgcga ccttcttcct ggcaccggcg gacaactgcg
     1921 ccgaggcggc gggccgtgag ccgtccgggc tgcaggtcgt caaggtcagc actttcgatg
     1981 acgcactggc tgcggtgacc gcgatcggga agggaaatac gtcggggctg ccgcactgct
     2041 gagccgttcg cggcgcggtc ggccacggat gttcaccagc cgttgccgaa tgggtgaatg
     2101 cgaagcgcgt cgctagcggt aggtagattg acctggtgga tgaggaaaag tggtttgacg
     2161 gcgataccga ccagcccccg aacaccccgc ccccaggcgg ccgaccagta gtagcgcccc
     2221 gacgcagtcc actcggggtg acggtcgcgg tgctcatcgg tctcggaatc gtcgtcacgg
     2281 ccgtggccca ggtctggacc gaggtcatgt ggttcgactc ggtcaacctg cgctcggtgt
     2341 tcacgacgca gctcggtgcc cagctcctgc tgggcatcgg tggcggcctc atcaccgccg
     2401 cgctggtctg gtcaagcctg cacttcgccc accgggcccg gcccatctac ccgccggtca
     2461 cgccccaaca ggatgccctc gaccgttacc gcgaggcgct cgagccgatc cggcgactgg
     2521 cgacgctcgt cattccgctc atcgccgggc tggccaccgg gctcactgcg tcgtcgcagt
     2581 gggagacctt cctgctctgg cgcaacgccc agccgttcgg gatcaccgat ccgcagttcg
     2641 gcctcgacct gggcttcttc gtcttctcgc tgccgtggct gaccttcgcg gtgggcttcg
     2701 tcaccatggc agccgtcctg gccgccatcg ccgcggcctt cacccactac gtctacggcg
     2761 ggctgcagtt ggccaaccgg gggcgtgaca cgacccggtt cgccctcctg cacctgtcca
     2821 gcctgttcgc cgtcatcgtg ctcttgcgcg cggcgagcta ctggctggag cgctacaccc
     2881 tctcgctgtc gacgtcggcg ctgatgaccg gcatccaata caccgacgcc aacgcggtcc
     2941 tgccgaccaa ggcgatcctc gccgtggcct cgctcatgtg cgccgcgatg ttcctgtcgg
     3001 tcatctggac ccggtcctgg cggctgcccg tcgtcggcac cgtcctgttg ctcatcacct
     3061 cggtcgtcgt tggcgggatc ttcccggccc tcatccagag cctcaaggtc aagccgagcg
     3121 agaagtcgct cgaggccccc tacatcgccc gcaacatcga ggcgacgcgc gcggcatacg
     3181 ggctcggggc tatcgaggtg tcgacgtacg tgccgagcga cgaggcgagc gccgagaacc
     3241 tgcgcgcgac cgcggcagcc atcccgggca tccgcatcgt cgaccccaac gtcgtctcgc
     3301 cgaccgtgcg ccagaagcac gccgtgcgca actactacgc gttccccgat gcccttgacg
     3361 tcgaccgcta cacgatcgac gggaaactgc gcgacgtcgt tgtcgccgct cgcgagctcg
     3421 acctcaccgg tgtgcctgcc gggcaacgca actggctcaa cgaccacacc gtctacacgc
     3481 acggttacgg catcgtcgcg gcatacgcca accagcagga ctccgagggg aacccggtct
     3541 acgtcgaggc ggcgatcccg gcggggaccg ccctggggga gtacgagccc cggatctact
     3601 tcggtgagca gtccccggac tacgccatcg tcggggctcc tgacggcgcg gcaccgcggg
     3661 agttcgacta cccggcccag gtcgagtctc agcaggtcaa caacacgtat gccggcaccg
     3721 gcggcgtccc gttggacaac acgatccgca agctggccta cgggttgaag taccgcgagc
     3781 tcaacttcat gctgtccgac gcggtcaacg cctcgtcgcg gatcatggac taccgcacgc
     3841 ccaaggaacg ggtcgggcgg gtcgctccgt ggttgaccct cgacggcaac gcctacccgg
     3901 cgatcgtcga tgggcgcgtg ctgtggatcg tcgacggcta cacgacgacg gccaactacc
     3961 ccaactcggg gctcacctcg atggcggcct cgacgcgtga ctcgacgacg agcacccgac
     4021 gcagcgtcac gagcctggag cagggccagc tcaactacat ccgcaactcg gtcaaggcga
     4081 ccgtcgacgc ctttgacggg tctgtccacc tctattcgtg ggacgacgca gatccgatcc
     4141 tcgcggcgtg gcgctcggcc ttccccggag tcgtcgaacc gcgggaaaac atctcggcca
     4201 gcctcatgtc gcacctgcgc taccccgagg acctgttcaa gatccagcgg accctgctgg
     4261 cgaagtacca cgtgcaggac cccgactcgt tctacggcgg gcaggacttc tggcgcatcc
     4321 cgcaagaccc gacgcaggag acggcgcgca ccgaccagcc gccctactac ctgtcgatcg
     4381 ccatgccggg tcagaccgac ccgaacttct cgttgacgac gaccttcatg ccgaccggta
     4441 accgggaggt gctgaccggc ttcctcgcgg tcgactccaa ccccggcaac cagccggagg
     4501 gcaaacgcct ggacggctat ggcacgttgc ggttgctcga actgccgccc aacagctcgg
     4561 tcaagggtcc gggccaggtg cagaacgaca tcgcctcgtc caaccagtcg tccaaggcgt
     4621 tcaccctgac cctgtcgcag ttcatcaaca acgcgcgcca gcagggctcc gtggtcaccc
     4681 tcggcaacct gctgacgttg ccgatgggcg gcgggatgct ctacgtcgag ccgatctacg
     4741 tccagggcac ggccgcctcg cagttcccgt tgggccgagc gatcgtcgtg gccttcggaa
     4801 acaagctcgc gtggtccaac accctcgacg gggcgctcaa cgagttgttc ggcggccaag
     4861 tggtcccccc gacgacgccc accacgccga cgacgccgac aaccccgacg acaccgacgc
     4921 ctgggccgac cacgccgacg acgcccgccc cgggcgacct ggcgcaggcg atcgccgacg
     4981 cacagaaggc ctacgccgac ggagagaccg ccctcaaggc gggagacttc gccgcttacg
     5041 gtgacgccca gaagcggctc aaggacgctc tcgctcgggc cgcggccgcc tcgccgaccg
     5101 ggtcgctcac cgtgaccccg tcacccaccg catcgggctg acccaccctc acccgcatgc
     5161 tccggtatgc cgcccgctca cctgagcggg cggcataccg ctgcgtgggg tggggggtgt
     5221 ggtgacggat ggtgacggtc tcgcggcgtg gtgaaggtgc gggtcgagca gcggccaaaa
     5281 ccgggcgatt tggctgtgcg gcaaggctcg cgtaaggttc tacttaccga cgcggggtgg
     5341 agcagctcgg tagctcgccg ggctcataac ccggaggtca caggttcaaa tcctgtcccc
     5401 gctacaaaag ttcaggctcg gcttccttcg ggaggccggg cctgaagcca tctcaggcgc
     5461 gatgtgcccg ccgtgtgggc tcggccctca gggttcctgg acgctacccg caggtcgagg
     5521 caagggccgc atccgttccc atcgccgacc tcttcgtggc cgccacgacc atcgccgaga
     5581 tcgagcgcgg agtctttggc aaggaacgat ccgacccacc ccagggtgcg gtcttgcgcc
     5641 ggtggttcga agaccgggtg cttacagcgt tcgccgggag ggtgcttccg ttcgacctgc
     5701 ccgccgcgcg actcctggcc acctaccgag tcccgcagca cgctccgctc gatgccgcac
     5761 tcattgcggc catcgctcag gccgcgcaga tgaccgtcgc cacccgccac gcccgacact
     5821 tcgaagcgcg cttgcatgag gctagcgacg cttgtatgcc gttacagcct catgcaagtg
     5881 ccaccaagct catacaagct gggcgcggcg gaccggggac cagcgccgcg ggcggccagc
     5941 tcggtggagg gtaacgacag acgaacgaag gcccggacca agaggtccgg gccttcgtca
     6001 tgccgtgggt caagcggtca gatgccgcga accttctcgg cctgcgggcc cttctggccc
     6061 tgggtcacgt cgaactcgac gcgctggccc tcgtcgagcg agcggtagcc gcccgagtcg
     6121 atcgcggagt agtggacgaa gacgtccggg ccgcctgcat cctgcgcgat gaagccgaag
     6181 cccttttcgg cgttgaacca cttgacggtg ccttgtgcca tgtcttgatg acttcctgtt
     6241 cgttcggagg gcaccaccgt ggtgccgtcc aggccgcggt gtcgcatcac cgggatcccg
     6301 aggaagcctg gaaatgacga ggcccgccgt cggaggcggg ccctgctctt acgagcgaac
     6361 tgcaactgca accgagcgcc agtttagcag ccgtcggcga cttgcggtcg attcgaggcc
     6421 actcaatcct gcggcgcgtc ctgcgccggc cagcgaggcg tcgctgtcgg cccgcgcacc
     6481 ccctcgaacc accccactgc acgcagcacg ccgacgtcgt cgaaccgccg cccggccacc
     6541 tgcacaccga ccgtccaccc gtcgtcgagg aaccccgcgt tgaccgagca ggccggctga
     6601 ccggacatcg agaacggcac cgtgaacccg atgtgcgcca tcccctcgtc ggcctcgccc
     6661 cacggcatcg gccactccgc ggggaaggcc gccaccggcg cgacgggaga cacgatgagg
     6721 tcgtatgccg cggtcgccgc caccgtggcg gcccgcatcg ccatcaccga ctggtaggcg
     6781 cgcaggacgt cgaggccact ggtccgagcc cctccctcgg cccaacgtcg cacgaacggc
     6841 aagacgcgag aacggttttc cggcgtcaac gtctcaaggt cggccagtga ccgcacccgc
     6901 cagaacaggt cgatgtcggc cagcatttgt ggcgtcagcc acggctggat ctcctcgacg
     6961 accgccccgc cgctctcgaa gacccgtccc gagctctcgg cgacggcgcg cacggcgggg
     7021 tcgcacggca taccggcgcc ggcatcgagc cacagaccga cccgcagccc gcgcaccgac
     7081 gcggtctcga ccgaccagtc ggcctcgtgc gccggcagcg ccgtccagtc gcggtcgtcc
     7141 ggccgggcca gcaccgacat cgccaccgcg gcatcggcgg tcgtgcgggt cagcggcccc
     7201 gcaacccggc cgaggtaggg cgcgtccagc ggcactcgcc cgaacgacgg tttgtgcgcg
     7261 acgagcccca accaggtccc cggcaggcgg atcgacccac cgatgtcggt gcccacgtgg
     7321 atcgccccgt atgccgcgac cgccgcggca cccgcgcccg ccgacgagcc gccggtggtg
     7381 agcgccgggt cccatggcga gcgggtgatg ccgtgcagcg acgagacccc ggacgagagc
     7441 attccccagt cgggcatcgt cgtggacccg acgatgaccg cgcccgcctc gcgcaaccgc
     7501 tcgacgacgg gggagtcgcg gaccggcacg acgggggtcg cgccggcgca gccgccctgc
     7561 atcgcgacgc cagcccgcgc gaggttctcc ttgacggtga ccggtatgcc gtccagcggg
     7621 ccgagcggct caccgtgccg ccagcggtcc tcggactgcg ctgcggcaag cgccgattcg
     7681 cgatcgaggg cggctgtcgt cgctgcgtcg tggcgcacca gggcgttgat cgtcggctcg
     7741 aaggcggcga tccgggcccg aaccgcagac aggacctcga ccggcgacaa cgaccctgac
     7801 ccgaaggccg tcgacagcgt ggcgacgtcc cacatcgctg gatcggtcat cgcgttcctc
     7861 ccatcgtggc cccgacgcag gtttaccact gaaggggagc gcgcggcata cccctcgggc
     7921 gcgggctcgt gggcgcgaag gtgacggttg ggtctcgaag gcccaagccc ggaaggcggg
     7981 cctgtccccg cgcgttccct acccgtagcc tgtgggactg ctacaggaca tcgtcgccgg
     8041 acttccaggg agccccccgt gacccatcgc ctgcctcgcc tcatcgcgcc tttggccgcg
     8101 ttggccctcg ccctcaccgc ctgcaccgca ggcgcaggga ccgcgccagc gccgaccgcg
     8161 accggcgggt ccacggccgc cggaggcccg tcgaccgaca agatcaccct cgggctggtc
     8221 gccgagccgg cgagcctcga cttcaccaag accgacggcg cggcgatccc gcaggcgctg
     8281 ctggtcaacg tctatgaggg gctggtcaag ctcgaccagg acggcaagat cgtcccggcc
     8341 ctcgcggcct catggaaggt ctccgacgac cacaagacct atacgttcga gctcaccgac
     8401 aaggcgaagt tcagcaacgg cgccgccttc acggccgacg acgcggtctt ctcgatcaac
     8461 cgggtcaaga ccgactggac gacctcgctc aaggcggcca tggacgtcgt cactgaggcc
     8521 aaggcgatca gcccgacgca gctgcaggtc accctgtcgg ccccgagcaa cgactggctc
     8581 tatcggatga cgacccacat cggggcgatg ttctccaaga ccggcgtcga agacctggcc
     8641 aacaagccgg tcggcaccgg gccctacacg ttcacctcgt ggaaccgcgg cgactcgatc
     8701 acgctgaccc gcaacgacgg ttactggggc acgaagccct actaccaggc cgtcgtcctt
     8761 aagtatttca aggacgccac cgcgctcaac aacgccctgc tcaccggaac gatcaacgtc
     8821 gtgacgaccg tgcaggcccc cgaggcgctg ggccagttca cctccaacgc gaagtaccag
     8881 gtcatcgagg gcacgaccaa cggtgaggtg ctcctgtcgt tcaacaacgc gcaggagatc
     8941 ttcaaggaca agaaggtccg ccaggccgtc cgctacggca ttgaccacaa ggccctcatg
     9001 gacacctgct gggccggcaa gggcacgctc atcggctcct tcgtcccgcc gaccgacccg
     9061 tggtatgagg acctcaccgg cgtcactccc ttcgacaagg ccaaggcgac tgcgctgctc
     9121 gccgaggcgg gcaagacggg cacgacgctg cgactgcggg tcccgaccct gccctacgcc
     9181 acctcgtgcg gccaggtcgt caaggcacaa ctcgaggccg tcggcttcaa ggtcaccctc
     9241 gactcgctgg agttccccgc cgcgtggctc acgacggtct tcaagggcgg cgacttcgac
     9301 atgtcgatcg tcgctcacgt cgagccgcgt gacctcaagg gcgtcttcgg caaccccgag
     9361 tactacaccc actacgggac ggccgacatc caggcgctcc tcaaggccgc cgacgagggg
     9421 tcggccgacg accaggtgac caacctcaag aaggccgccc gactcatcgc cgaggacgcc
     9481 gcgggtgacg tgctgttcct gctgcccaac ctcatggtgg ccgacaaggg catcactggg
     9541 ctgccgaaga acggcatcgc ggtcgccttc gatctgtcca agctcgccaa ggcctgaccg
     9601 gccagaacgc ctcaggtatg ccgcccgctc acctcgagcg ggcggcatac ctctgcgtgt
     9661 cgttggcgag cagcacccgc agcgtgatct cggttgcccg ggcacaccga atgccaatgg
     9721 tcgggcgctg gcagcaggtc cgtgcgtggc gggccctgag cgtggtgacc aatggcattc
     9781 ggtatgccgc gcgctcactt cgcgggggcc gcgcggcgta cttcccgtcc gaccgagcca
     9841 catgacaggc tggcgccatg ctcctgcgca tcgccgagcg gaccgcggtg ctcctggtga
     9901 gcctcggggt gagttcggcg ttggtgttcg ggttcatggt gctgctccct ggtgacccgg
     9961 cccgggtggc gctcggagtc aacgcgaccg aggccgacgt gtcgcggctg cgtctggagt
    10021 tcgggctgga gcggccgctg gtcgagcaat acctctcgtg ggtaggcgga ttggtgcatc
    10081 tggaccccgg catctcctac gtcagccgcg cccagatcgg accccagatc gccgatcgcc
    10141 tgcaggtgag cctcatcctg gtcgccgcct cgatgaccat tgccgtcgcg ctcgccctgc
    10201 ccttcgggac cctgatggcc gtccaccacc gcaaggcctc cgggttggtc ctctcggccc
    10261 tgtcgcaggt cggggtcgcc gttccggcgt tcctcgccgg gatcctgctc atcaccgttt
    10321 tcgcggtcca actgcggtgg ctgccggcga gcgggtggac gccaccggca tacgacccgg
    10381 gaatgttcct ccagcaactg ctcctgccgg ccctctccct cgggctcgtc cagggcgcgg
    10441 tgctgacccg ttatgtgcgt tcggcggtgc tcgacgtgct ccgggaggac ttcctgcgca
    10501 cggcgcgcgc caagggactc acccccatgc gggccctcgt gcgccacggg ctgcgcaacg
    10561 ccgcagtccc ggtcgtcacg gtcctcgggc tgcagttggc gaccctgctg gtcggcgcgg
    10621 tcgtcatcga gcgggtgttc gtcattcctg gcctcgggtc cttgctgctc gacgcagtcg
    10681 gcaaccgcga cctcatcgtt gttcaggacg tcgtcatggt cctcgtcgtc gccgtcctca
    10741 tcgtcaactt cgtcgtcgac ctgctctatc tggcgatcga cccgcgcctg cggacggtcg
    10801 cccgatgagc cgcctgtcgc gaggggccga agccgtgatg ccaccgcccg gtcggcgttc
    10861 gcgtgcgggt gtcgaggcga cgttgctcgt ggggggcggg atcgtcgcgg cgatcaccgt
    10921 cctcgccctc gtgtcgttgg tgtggacgcc ctacgacgtc ggcgagatca acgccaccga
    10981 gcggctcgcg acctcgtcgg cgcggcactg gttgggcacc gacaagttcg gccgcgacgt
    11041 cgtgtcactg ctcatggtcg gttcgcgcac cacgctgctc gtcggtctcg tctcggtcgg
    11101 ggtggctgct gtcatcgggg ttccgctggg gatcctcgcg gcgatggtgc ccaagtggtg
    11161 gggcgaggtg ctcatgcggg ccaacgacct gctgctcgcg ttccccgccc tgctgttggc
    11221 gatcatgctc acggcggtcc atggccaagg cgtcgtcacc gccatgatcg ccatcggcat
    11281 ctcgagcatc ccgtcgttcg cccgggtcgt gcgcagcggg gccatcgggg tgatgaagac
    11341 cgaatacgtc gtcgccgcgc gcgctgccgg tcgccgaccg atcgcgatcg cgctgcggca
    11401 cgtgttgccc aacgttggca gcctcgtcat cgtccaggcc tcggtgtcct tcgcgatcgc
    11461 cgtgctcgcc gaggcagccc tgtcctatct cggcctcggc gcccctccgg ggactccctc
    11521 atggggacgg atgctgcagg aagggcagga gttgctgttc gtcgcgcccc gcttggaggt
    11581 cttcccgggg gtcgcaatcg ccctcgcagt gctgggcttc aacctccttg gggacgggct
    11641 gcgcgaccac ttcgacccgc gactggagga ccggcggtga ccgcggcacc ggagcacacg
    11701 gcatacccag tggggggtat gccgcccacg cggactcccg ctgacacgcc gccgccggcg
    11761 gacgtcgcgc gcgtgcgcga cctcgcggtg actgtcggcc acaagccgct gctcatcgac
    11821 gtcgacctca ccgtgcgccg tggcgagcgg gtcgggctca tcggcgagtc gggctcaggc
    11881 aagtcgctca ccgcgctcgc gatcatgggg ttgctgcccg aggatctggc gccgacgggg
    11941 tcggtcgtgg tgaccgggca ggggccgggt ggtatcgcgc ggatgacctc ggtgctggag
    12001 gcgggggagc acgggctcgc ggcgatgcgt gggcgggatg tcgcgatggt gttccaggag
    12061 ccgatgaccg cgctcaaccc gacgatgcgg gtgggcgccc aggtcgcgga ggtgatgacg
    12121 atccaccgga cggtggccga tcgcggggcg gcggcgcggc gggcggtcga gttgttggac
    12181 aaggtgggct tgcccgatgc ggcggaggcg gctcgcgcct atccgcatca actctccgga
    12241 ggtcagcgcc agcgggtcgt cgtggcgatg gcgctggcca acgacccggc gctgctcatc
    12301 tgcgacgagc cgaccacggc gttagacgtc acggtgcagg cgctcgtgct cgacctcatc
    12361 gtgcgtgggg tggaggcgcg tgacgcggga ctgctgttca tcacgcacga cctggccgtg
    12421 gtggcgagcg tctgcgagcg ggtccttgtc atgtatggcg ggcgggtcgt cgaggccggc
    12481 ccggtcctcg acgtcttcac gcggccccgg catcggtata cgcgcgggct catcggagca
    12541 tctgatctgg acctcagcag gcctgagggc gggctctaca cgatccaggg atcggtgccg
    12601 gcggccggtc gcttccctgc ggggtgcgtc ttccgcaacc ggtgcacgca tgccacggtg
    12661 gagtgcgagg cggtgccgcc gtgggttggg tcagggcccg aagaagggtt cgcctgcgct
    12721 catccggtcg ttcaccacga aggtggtggc tcatgacgga cgtggcgatc tcggtgcgcg
    12781 acgtggtgcg cgactatcgg cggcctcgaa cgtcgctgac caagccgggc tcggtcgtgc
    12841 atgcccttcg cggggtgagc ttcgacgtgg cgcgtgggca gcggtttggc atcgtgggcg
    12901 agtcggggtg tggcaagtcc acgctgttgc ggatcatcgc cggcctcgac caaccgacct
    12961 ccgggtcggt ccaggtcggg ggagtggaga tctcccggct gcccgagcgg cggctgaggt
    13021 tcttgcgggc ccagttgcag ctcgtcttcc aggaccccat ggggtcgctc gacccccgga
    13081 tgcgggtccg ggacatcatc gccgaacccc ttgtggcgca agggcatcgg gacaacattg
    13141 gccgcgtgca cgagctgctg gacgccgtcg ggctgcagcc tgaggcggcg gatcggttcc
    13201 cgcatcagtt ctcgggtgga caacgtcaac ggatctcgat cgcccgggcg ttggctcctg
    13261 acccgcgcat cctcgtcgcc gacgagccgg tcagtgcgct cgacgtgtcg gtgcgggccc
    13321 aggtgctcaa cctgctcgat gagttggtgc aacggctctc gctcaccttg gtgttcgtct
    13381 cccacgacct gtcagtggtg cgacatgtct gcgacacggt ggccgtgatg agccgaggtg
    13441 agatcgtcga gatggggccg atcggcgacg tgtatgacac gccggcccat ccgtattcgc
    13501 gccaactcgt cgccgcagct ccccggctgc aggccgctct ggccggccga accgcggcgg
    13561 tcctggctgc ggaaaccctt gcggcccggg ccttgtcgag caacgcctcg tctcgcaacg
    13621 ccgtgtctcg caacgccgcc gactctgact cgaaacccac tccgccgacg cccgagcaaa
    13681 cgacaccccc accgaaggac gcgccatgac cgagaacccg gccatccgcc cagggcggac
    13741 caactcgctg accgacgtgg acggcatacg tgtcggtcat gcgacccgca tcggcgacgg
    13801 ctggctcacc gggacgaccg tggtgctcgc gcccgacggc ggggcggtcg ggggcgtcga
    13861 cgtgcgcgga ggcgggcccg gcacccggga gaccgatctg ctcgacccgc gcaacatggt
    13921 cgatcgggtc catgcggtcg tcctgtccgg ggggtcggcg ttcgggctgg ccgcggccga
    13981 cggtgtgatg gttcgattgg cttctgcggg agtcggattc ccggtcggag ggcccagcga
    14041 ggtcgtgccg atcgtgcccg ctgcggtgat cttcgacttg gggcgcgggg gatcgtttgg
    14101 caagcgcccg gacgccgcca tgggcaacga cgcgctcgtc gtcgccaacg acgggccggt
    14161 ccaacaaggg tgcgtcgggg cgggcacggg cgcccgggcc ggaggtctca aaggcggagt
    14221 cgggtcggcg tcagcggtcc tgccggacgg gacgacggtc gccgcgctga tcgtcgtcaa
    14281 cgccgtcggc tcctgcgtcg acccactgac cggcgagttg tatgccgccc gcttcgggct
    14341 tcccggcgaa ttcccttctc tggggtcggt gtccgaggct gatctggctg cggcgcggga
    14401 gcgggctgcc gccgccgggc cggggttggg gctcgggccc gggctcgcga cgacgatcgg
    14461 ggtcattgcg accgacgcca cgctgaccaa ggcgcagtgc gcgaaggtct ctgggatcgg
    14521 ccacgacggg atggctcggg cgatccggcc ggtgcacacg atgttcgacg gcgacacctt
    14581 gttcacgctg gcatccggcg ctcggggggc tgcgggtgct cccgacccgc tggccttcca
    14641 cgcgctgctg gatgctgcgg gggactgtgt cacccgggcc atcggccacg ccatgctgtc
    14701 ggccaccacc gtgacgccgc ccgctggtga gatccgttgc ctgcgcgacg ccttcccctc
    14761 cgcctttgcc cactgaccgc tcgcgtcagg cccgcctccc tcttcccgaa tccctcacac
    14821 tcgccggtga tctggacgaa accccggcct gacttccccg aatccctcac gctcgccgat
    14881 gatctggg
//
LOCUS       DAOMZY010000036        14300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_3866_length_14300_cov_6.247666, whole genome shotgun sequence.
ACCESSION   DAOMZY010000036 DAOMZY010000000
VERSION     DAOMZY010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 14300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14300
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3866_length_14300_cov_6.247666"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PLK64_03525"
     CDS             complement(<1..658)
                     /locus_tag="PLK64_03525"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPV78840.1"
                     /translation="MTDGRHRVALVTSSFHPHVGGVEEHVRQVALALRAQGHDVVVWT
                     VDRGEHLGVQTVDGVLVRYLPTPLPSATPTGVARFARVAPAAWHAWREALRADRPQVL
                     HVHCFGPNGVYAAALARTTRIPLVVTSHGETTADDHDAYGRSWLLRTTLTRAVRRAVC
                     TTAPSQAVLADLRERFGLEGGSVVPNAVSDAASLGSDSPMAVVEATADTSPVIVAVGR
                     L"
     gene            727..1776
                     /locus_tag="PLK64_03530"
     CDS             727..1776
                     /locus_tag="PLK64_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPV78841.1"
                     /translation="MPLLVLVTAAAAAPMGAQVYEEQLAARAQSALTAIDPQWQVQRR
                     IIRSMRSPLPGTGRLPMGWLAGAGERGRAAVGRLAYPRHAVVHRTSLELPPAPLEVVT
                     LHDVVAWRFADESAPVRSAAAELRRAAAVICVSEFSAGEAHDLLGVRNPVVVPNGVDP
                     AFLDAVAAPTEVLAGLGLTGPFVLHAGGAATRKNLDALAQAWPLVRREHPDLTLALAG
                     PPHPRRDALFSGMPGARLLGRLPDPLVPGLVAAASAIVVPSTYEGFGLPALEGMAAGV
                     PVVAARCAALPEVVGDGGLLVDPEPVALAQGLLDVLAGGPDVADLVRRGRERAGGFTW
                     ERSAAGHAAVWAALG"
     gene            1875..2903
                     /locus_tag="PLK64_03535"
     CDS             1875..2903
                     /locus_tag="PLK64_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HPV78842.1"
                     /translation="MSDNPQPGEAHGERRRNRRLLVVVLLMLAVPVVAVGGYLGWLNH
                     LVTSNIKFADLMPTGGPLVPVDGGAFDPSEPGEGGIPTAAVPAESAGDNYLLIGSDAR
                     SGLGGARSDVIIFVHVPIDKHNVSLIHFPRDMYVFIPGRGRNKINAAFAFGGAPLLVD
                     TVQGMLGVKIDHVAMIGFEGFKRMTDAVGGLDVYVEEGGKIDGHVFTQGMMHLDGEMA
                     LAFVRERYDLSQGDISRGRRQQAFLKALMLEAVSVETLANPAKLATFIDSATSNVIVD
                     RGFDVGQMRGELLSLNGLRPGDIRFLTAPLSGFGRSPYGASIDLVDWVKLTQLGTAIR
                     EDRLDSYS"
     gene            complement(2937..3860)
                     /locus_tag="PLK64_03540"
     CDS             complement(2937..3860)
                     /locus_tag="PLK64_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78843.1"
                     /translation="MALLAVDVALVSAAFRSPFRQPATAPVTGAAATVTGTVPGSAGG
                     TSTPAATSSTPAATGFSGSGSMVGVVPIDSARAWRFVQGACPGGGASLAHTDNGGRTW
                     EDVPIPFGAISRVQVTDAAKVVVVGTEGAGCRETALRSTDTGQTWASGGALALWYRSL
                     ATPTAVVNSNGRAGTPCGNGKALSVSALSATSAAVLCESGKVVETADGGGNWVEVSRG
                     LSALAVDARVEASKLAALVAYVADGCSGVQVASIAAGATTKLGCVDLGGRSNDGLLTH
                     VALSVEGNAGWLIVDGDTYTSDDGGKTWSTP"
     gene            4048..7698
                     /gene="mfd"
                     /locus_tag="PLK64_03545"
     CDS             4048..7698
                     /gene="mfd"
                     /locus_tag="PLK64_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPV78844.1"
                     /translation="MTSTAPSAGTLAHLPTDWAARHTDWGLGTHVGDQTALLDLACVP
                     GARPLVLAVLAGALPVGAQVLAVTATTREADDLAAALAAFLDPALVEVFPSWETLPHE
                     RLSPRNDTVGRRLAVLRRLAHADAHAPGAAGPLKIVVAPVRAVLQPIARGLGDLTPVA
                     LAAGDERPLERVVEDLVAAAYSRTDLVERRGEFAVRGGILDVFPPTEEHPLRVEFWGD
                     TVEEIRWFRVADQRSLEVAEGGLWAPPCREVLLTDDVRERARDLATRLPGVADLLEKV
                     AEGIAVEGMESLQPVLVEAMQTLPDTLGAGSLIVLADPERIRTRAHDLVATSEEFLEA
                     SWSNAAAGNAVPIDLRGVLDEGSYRTLAGLRQDAITSGMPWWSLTAFASDAELTDFLD
                     DDTSEQRLDLGLREPDAYRGDTEAAVADLGLWIHDGWTVLTVTEGPGLARRVHEVLAE
                     RGIPSRLVGHEAESAPGVVTVTTGALGRGFVDETGQFALLTETDLTGSAGSATTTKDM
                     RRMPSRRRAQVDPLSLRPGDYVVHEQHGVGRFVEMTARTVGGATREYLVLEYAASKRG
                     QPGDRLYVPTDQLDQVTKYVGGEAPTLHRLGGSDWAQTKARARRHVKEIAADLIRLYS
                     VRMSTVGHAFAPDTPWQRELEDAFAYVETPDQISSIDEVKADMEREVPMDRLICGDVG
                     YGKTEIAVRAAFKAVQDGKQVAILVPTTLLVQQHLQTFTERYAQFPVTVKALSRFQST
                     TEAGRVVDGLADGSVDVVIGTHRLLTDKIRFKDLGLVVVDEEQRFGVEHKEKLKTFRT
                     AVDVLAMSATPIPRTLEMAVTGIREMSTLATPPEERHPVLTFVGSYDEKQITAALRRE
                     LLREGQVFYVHNKVQSIEKAAARLRELVPEARIATAHGKMTEGRLEQVVLDFWEKRFD
                     VLVCTTIVETGLDISNANTLIVERADLLGLSQLHQLRGRVGRGRERAYAYFLFPPEKP
                     LTETAHDRLATIASHTDLGSGMQVAMKDLEIRGAGNLLGGEQSGHIEGVGFDLYVRLV
                     GEAVAAYKGEAQAPPAEVKIELPVDAHLPHDYVPGERLRLEAYQKLASVQDEAGVAAI
                     EEELRDRYGMPPEPVTNLIEVARLRTVARLAGLSDISAQGAFVRFGPVPAEAMPDSRR
                     LRLQRLYPGSIVKDQLGHLLIPRPMTARVGGKPVRDTAVLRWAADVIRAVLVDNVGDA
                     ARVATNGKDLRP"
     gene            7695..8225
                     /locus_tag="PLK64_03550"
     CDS             7695..8225
                     /locus_tag="PLK64_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78845.1"
                     /translation="MSPRTPRRLGTLPAALIGLLVATLIAGCGAAGRPGTALVVDGQS
                     YTPSQIVETTAQINEALKAGGEAATEAQIVNLLMISPFVLAEATSRGLWGPDNQYNSF
                     MTAIHNPTPVTVAVVQASAAYASLDEEGKAALLKDIKAASIAVDPRYGSVDYSTGFLG
                     MPTPDWIVRTTTAPEK"
     gene            8236..8898
                     /locus_tag="PLK64_03555"
     CDS             8236..8898
                     /locus_tag="PLK64_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HPV78846.1"
                     /translation="MTGGPGPSVPSGAALLDLVAVMDRLRSPGGCPWDAEQTHASLVP
                     YLVEETYEAVEAIESGERDHMAEELGDVLLQVVFHARVAQEDEVAPFDIDDVAAGIAA
                     KLRRRHPHVFGDVVADTPEAVAANWSVIKAAEKAAKGSGAAPPGPLHGIPAGMPPLAK
                     AAAMVARLDRAGRGDLVDAAAAGSDPGSRLLAVVADVVRRRDDPDAVLRATLRSLSDA
                     AG"
     gene            8995..10281
                     /gene="eno"
                     /locus_tag="PLK64_03560"
     CDS             8995..10281
                     /gene="eno"
                     /locus_tag="PLK64_03560"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPV78847.1"
                     /translation="MASIEAVGAREILDSRGNPTVEVEVLLDDGTVARAAVPSGASTG
                     AFEAYEKRDGDKGRYLGKGVEKAVDAVLDEINPKIMGFDASEQRLIDAEMLAMDGSKN
                     KENLGANAILGVSLAVARAAADSANLPLFRYVGGPNAHVLPVPMMNILNGGSHADTNV
                     DIQEFMIAPIGAPTFKEALRWGAEVYHTLKKVLHDRGLSTGLGDEGGFAPNLESNRAA
                     LDVIVEAIEKAGYVPGKDIALALDVAATEFYKDGAYQFEGAAKTSAEMTAYYGELVAA
                     YPLVSIEDPMSEEDWDGWKAITVALGDKVQIVGDDLFVTNPERLQRGIDTQTANALLV
                     KVNQIGSLTETLDAVDLAHRNGYRCMMSHRSGETEDTTIADLAVATNCGQIKTGAPAR
                     SERVAKYNQLLRIEEELDDAAVYAGAGAFPRFAAKG"
     gene            10324..10875
                     /locus_tag="PLK64_03565"
     CDS             10324..10875
                     /locus_tag="PLK64_03565"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HPV78848.1"
                     /translation="MTTPARRTRVRPGTTATTGRRPGASRPAPAPRTREATPDDDRRG
                     PAWLRWVILGAIAVVLLVTLVPTARSLARQRAEIDALATQVAQQQSQVASLQAEQERW
                     TDPAYVEQQARDRLKFVKVGDRSYTVIDPQEAARPPVSGASVAAPQANGDAPWYGQIW
                     QSVQLADRPTAGMLPVTAPPTAP"
     gene            10872..11411
                     /locus_tag="PLK64_03570"
     CDS             10872..11411
                     /locus_tag="PLK64_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HPV78849.1"
                     /translation="MTPPTEPTGPTGPTEPEDLAAIAGQLGREPRGVLGIAHRCPCGR
                     PDVVRTAPRLPDGTPFPTTYYATCPRLTAAISTLETQGLMRDMQARLADDEDLAAAYA
                     RAHTAYLADRATLASGGDVPEIAGVSAGGMPDRVKCLHVLVAHSLAAGPGVNPFGDEA
                     LALLAPWWDAGPCVGAAEE"
     gene            11464..12465
                     /locus_tag="PLK64_03575"
     CDS             11464..12465
                     /locus_tag="PLK64_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HPV78850.1"
                     /translation="MVPATAPRATSAAGEVRVGAIDCGTNSLRLLIADIDPQTGKMLE
                     LVRRMEIVRLGCGVDKTGVLAPEALARTLAMTRDYATQCREYAVHRIRFVATSASRDA
                     RNADEFVAGVRAAFGDLDVAPEVVSGQEEARLSFLGATGDLIAAQMEGPYLVVDIGGG
                     STEFVRGTHTVEAARSVDIGCVRLTERHLRSDPPTPDEIAAAIVDIDRAIDLAIETVS
                     LREVRTLVGLAGSVTTVAAQHLDLRDYDPGAIHLSVATPPQIQAACEQLMGMTRAERA
                     AKGFMHPGRVDVIGAGALVWSRVIARVMRQTGLGQALPVATSEHDILDGIALSLVGR"
     gene            12476..>12750
                     /locus_tag="PLK64_03580"
     CDS             12476..>12750
                     /locus_tag="PLK64_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPV78851.1"
                     /translation="MPQALPHPVTGDLSGSPAPPGSGWPGDPATRHTPRARDAEQVVS
                     LARSASDLATLDARISVCRACPRLVQWREQVAGDKRAAFADQPYWGRP"
     assembly_gap    12751..12850
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12851..13183
                     /locus_tag="PLK64_03585"
     CDS             <12851..13183
                     /locus_tag="PLK64_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPV78852.1"
                     /translation="PTVSERATCAPWLDRDLALAAPTVRSILALGGLAWDASLGAARR
                     LGWQVPTPKPTFGHGAMAQLREPGGRVVRLVGSYHVSQQNTFTGRLTESMLDSALALL
                     AWPDTVAE"
     gene            13180..13713
                     /locus_tag="PLK64_03590"
     CDS             13180..13713
                     /locus_tag="PLK64_03590"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPV78853.1"
                     /translation="MSTTPTPVLAVTVLGDDRPGIVAEVTAAVAGLGGNLEDSTMTLL
                     RGHFAMVVLVRTPASVADVEAALAGIAAGGSLTVDVRELRESGMPPAPGRAWTLRVHG
                     ADRPGIVASITEVLAAHGGNIVDLGTRLGSDLYVLIAEVELPEAASGEAVAADLRAAA
                     AAVGVTVAFEPVDDEVL"
     gene            13710..14246
                     /locus_tag="PLK64_03595"
     CDS             13710..14246
                     /locus_tag="PLK64_03595"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPV78854.1"
                     /translation="MSSFVMPSLPEGRVLDVVRAPAPVLATPGADVDPAAPSVIQLAA
                     DLLATQAVSPGCVGLAAQQVGVAWRMFSVDVTGHPKTRTCHGEFVLCNAVVESASRKE
                     KGREGCMSVPDFTGDVARARRLVVRGQLPVTGEWVTLETDAFEAVCLQHEIDHTNGLV
                     FLDRVAGPQAVHPRRTYL"
BASE COUNT         2066 a         5170 c         4873 g         2091 t
ORIGIN      
        1 ccaaccggcc gaccgcgaca atcaccggac tcgtgtccgc cgtggcctcg acgacggcca
       61 ttggagagtc cgagccgagc gatgcggcgt cggacacggc gttgggaacg acggaaccgc
      121 cctccagccc gaagcgttcg cgcaggtcgg ctaggacggc ttgggagggc gccgtcgtgc
      181 acacagcccg gcgcacagca cgggtgaggg tcgtgcgcag caaccaggag cgtccatagg
      241 cgtcgtgatc gtccgcggtc gtctcgccgt gactggtgac gaccagaggg atgcgcgtcg
      301 tccgggcgag ggccgcggca tacaccccgt tggggccgaa acagtggacg tgaagcacct
      361 gcgggcggtc ggcccgcaga gcctcccgcc aggcgtgcca ggcggcgggg gcgacccggg
      421 cgaaccgcgc caccccggtc ggcgtcgccg acggcaacgg cgtggggagg tagcgcacaa
      481 gcaccccgtc gacggtctgg acaccgaggt gctcgcctcg atcgaccgtc cagacgacga
      541 cgtcgtggcc ctgggcgcgc agcgcgagcg ccacctggcg cacgtgctcc tcgaccccgc
      601 cgacgtgcgg atggaacgag gacgtgacga gagcgacccg gtgccgaccg tctgtcatcc
      661 gcgtcctctc ctggccactg gcccgtccac cccgcgcggg cggcgtcttg ttggcatcat
      721 ggcactgtgc cgctcctcgt ccttgtcacc gcagccgcag cggcgccgat gggggcccag
      781 gtctatgagg agcaactcgc ggcccgcgcg cagagcgcgt tgaccgcaat cgacccccaa
      841 tggcaggtgc aacgacgaat catccgctcg atgcgctcac ccttgccggg gaccggacgg
      901 ctgccgatgg ggtggttggc cggggcaggg gagcgcggtc gggctgccgt cggtcgactc
      961 gcctacccac gccacgccgt ggtccaccga accagcctcg aactgccccc ggctccgctc
     1021 gaggtcgtca cgctgcacga tgtcgtcgcc tggcggttcg ccgacgagtc ggctccggtc
     1081 cggtcggctg cggccgagtt gcggcgagcg gccgccgtga tttgcgtgtc ggagttctcg
     1141 gccggcgagg cacatgacct tttgggagtc cgcaacccgg tcgtcgtgcc caacggggtc
     1201 gacccggcgt tcctcgacgc cgtcgcggca ccgaccgagg tgctcgccgg tctggggctg
     1261 accggcccgt tcgtgttgca cgccgggggc gccgccaccc gcaagaacct cgacgccctc
     1321 gcgcaggcct ggccactcgt ccgccgggag caccccgacc tcacgcttgc cctcgccggc
     1381 cccccgcacc cgcgccgcga cgcccttttc tccggtatgc cgggtgctcg cctcctcggc
     1441 cggcttcccg acccgctcgt tcccggcctg gtggcggcgg cgagcgcgat cgtcgtgccg
     1501 tctacgtacg agggattcgg gctgccggcg ttggagggca tggcggccgg ggttccggtc
     1561 gtcgccgccc ggtgcgcggc cctgcccgag gtcgtcgggg acggcggcct gctcgtggac
     1621 ccggagccgg tcgccctcgc gcagggcctg ctcgacgtgc tcgcgggtgg ccccgacgtc
     1681 gccgacctcg tccgtcgggg ccgcgagcgc gcgggcgggt tcacctggga acgaagcgcc
     1741 gcggggcatg ccgcggtgtg ggccgcgctt ggctgaggac gggttcgtcc agcagggcgg
     1801 ttcggccccc cccggtccgt gcgatgtcag ccgatcaccc tcgaaggcca cggacacccg
     1861 cctagagtgg gccaatgtcc gacaaccctc agccgggaga ggcccacggc gaacgacgtc
     1921 gcaacaggcg cctgctcgtc gtcgtcctcc tcatgcttgc cgtcccagtt gtcgccgtgg
     1981 gcgggtattt gggttggctt aaccacctcg tcacgtcgaa tatcaagttt gccgatctca
     2041 tgcccaccgg agggccgctc gtgcccgtcg acggcggggc tttcgatccg tctgagcctg
     2101 gggagggcgg catccccacc gctgcggtac ccgccgagag cgctggcgac aactacctac
     2161 tcatcgggtc ggacgcgcgt tcgggtttgg gaggtgcgcg gtccgatgtc atcatcttcg
     2221 tgcatgtacc catcgataag cacaatgtta gcctgattca ctttcctcga gatatgtacg
     2281 tgtttatacc gggacgcggg cggaacaaga tcaatgccgc atttgccttt ggtggcgctc
     2341 cgttgttggt cgacacggtt cagggaatgc tcggggtgaa gatcgatcac gtcgcgatga
     2401 tcggcttcga gggtttcaaa cgcatgactg acgcggtcgg cggcttggac gtatacgtcg
     2461 aagagggagg caagatcgac ggccatgtat ttacccaagg gatgatgcat ctcgacggtg
     2521 agatggcctt ggcgttcgtt cgagaacgct acgacctcag tcagggagac atcagtcgtg
     2581 gtcggcgaca gcaggccttc ctgaaggccc tcatgctcga agcagtcagc gtcgaaaccc
     2641 tcgccaatcc ggcaaagctc gccacattca ttgactctgc aacgagcaac gtcatcgtcg
     2701 acagaggatt cgacgtaggt cagatgcgtg gtgagcttct ttccttgaac ggattgcgtc
     2761 cgggcgacat ccgcttcctc acggctccgc tgtccggctt cgggcgatcg ccttacgggg
     2821 cgtcgatcga cctggtcgac tgggtgaaat tgacgcaatt gggcacagcc atccgtgaag
     2881 atcggctcga ctcctactcc tgagcccagg gcgaaaaggg atcagggagc ccgatgtcag
     2941 ggggtggacc acgtctttcc accgtcgtcg gacgtgtagg tgtcgccatc gacgatcaac
     3001 catcctgcgt tcccctcgac gctcagggcg acgtgagtga gcagtccgtc gttgctgcgg
     3061 ccaccgaggt cgacgcagcc gagtttggtc gtcgcgccgg cggcgatcga ggcgacctgc
     3121 actcccgagc agccgtctgc cacgtaggcg acgagcgcgg cgagcttcga ggcctccacc
     3181 cgcgcatcga cggcgagagc gctcagaccg cggctgacct caacccagtt gcctccaccg
     3241 tccgcggtct cgacaacttt gccgctctca cacagcactg cagccgaggt cgccgacaag
     3301 gccgagacgc tcagcgcctt cccgttcccg cagggtgtgc cggcgcggcc gttcgaattg
     3361 accactgcgg tgggtgtggc cagggatcgg taccacaagg caagggcccc tcccgacgcc
     3421 caggtctgcc ccgtgtcggt cgagcgcagt gccgtttcgc ggcagcccgc accctcggtc
     3481 ccgacgacaa ccaccttggc ggcatcggtg acctgcaccc gcgagatcgc tccgaagggg
     3541 atggggacgt cctcccaagt gcggccgccg ttgtcggtgt gggccaagga ggcgccgccc
     3601 ccggggcagg cgccctgcac gaagcgccag gcccgcgccg agtcgatcgg cacgacgccg
     3661 accatgctgc ccgagccgct gaacccggtg gccgccggcg tcgatgaggt ggccgccggg
     3721 gtggaggtgc cgccagccga gccggggacg gtgcccgtga cagtcgccgc ggcgccggtg
     3781 accggtgccg tcgcaggctg gcggaacggg ctacggaagg cagcgctcac cagggcaacg
     3841 tcgaccgcga gcagggccac caaaccccac gttgcaagtc gcgttcgcga agccatcggc
     3901 accccgggag agacgagaag acacggagca cagtcgaagt ctggactgtg ctgtcgtcat
     3961 gctaggtcag cggggccgat ccggtcggcg gttcccgcgg cttgggcgac ttcgaccaaa
     4021 ttcacctggc cgtagagttg tgaggctatg acctcgaccg caccctccgc cggcacgctg
     4081 gcgcacctgc ccactgactg ggctgcccgg cataccgact gggggctggg cacgcacgtc
     4141 ggcgaccaga ccgcactgct ggacctcgcc tgcgtcccgg gcgcccgccc actcgtcctg
     4201 gccgtcctgg ccggagcgct tcccgtcggt gcccaggtgc tcgccgtcac cgcgacgacc
     4261 cgggaggccg acgatctcgc ggccgcgctc gcggcgttcc tcgacccggc gctcgtcgag
     4321 gttttcccca gttgggagac ccttccgcac gagcggctct cgccccgtaa cgacacggtc
     4381 ggtcgtcgcc tggccgtcct gcgtcgcctc gcccacgccg acgcgcacgc ccccggcgcg
     4441 gccgggccct tgaagatcgt cgtggccccc gtgcgggcgg tcctgcaacc catcgcccgc
     4501 gggctcggcg acctcacgcc cgttgcgctc gccgccggcg acgagcgccc gttggagcgg
     4561 gtggtcgaag acctcgtcgc ggcggcctac agccggaccg acctggtcga gcggcgcggc
     4621 gagttcgcgg tccgcggcgg catcctcgac gtcttcccgc cgaccgagga acaccccctg
     4681 cgcgtcgagt tctggggaga cacagtcgag gagatccggt ggttccgggt cgccgaccag
     4741 cgcagcctcg aggtggccga gggtggcctc tgggccccgc cctgccggga agtgctgctc
     4801 accgacgacg ttcgcgagcg cgcccgcgac ctggccaccc gcctgccggg cgtcgccgat
     4861 ctgctcgaga aggtcgccga aggcatcgcc gtcgagggca tggagtcgct gcaaccggtc
     4921 ctcgtcgagg ccatgcagac cctccccgac actctcggcg ccggctcgct catcgtcctg
     4981 gccgaccccg aacgcatccg cacccgcgct catgacctcg tggccacgag cgaggagttc
     5041 ctcgaggcca gttggtctaa cgccgcggcc gggaacgccg tcccgatcga cctgcggggg
     5101 gtgctcgacg agggctccta ccgcacgctc gcgggactgc gccaggacgc catcaccagt
     5161 ggtatgccgt ggtggtccct caccgccttc gcttctgatg ccgagctgac cgacttcctc
     5221 gacgacgaca cctccgagca gcgcctcgac ctcgggctgc gcgagccgga cgcctatcgc
     5281 ggcgacaccg aagctgcggt ggccgacctc ggtctctgga tccacgacgg ctggaccgtc
     5341 ctcacagtca ccgaagggcc gggtctggcc cgacgggtgc acgaggtcct ggccgagcgc
     5401 ggcataccgt cccggctcgt cgggcacgag gcggagtctg cccctggggt cgtcaccgtg
     5461 acgacgggcg ccctggggcg cggattcgtc gacgagacag gccagttcgc gctgctcacc
     5521 gagaccgacc tgaccgggtc ggcggggtcg gcgacgacca cgaaggacat gcgccggatg
     5581 ccgtcccggc ggcgcgcgca ggtcgacccg ctctcgctgc ggcccggcga ctacgtcgtc
     5641 cacgagcagc acggggtggg tcggttcgtc gagatgaccg ctcgcaccgt cggcggcgcc
     5701 acccgcgaat acctcgtgct ggagtatgcc gcgagcaagc gggggcagcc cggcgaccgc
     5761 ctctacgtgc ccaccgacca actcgaccag gtgacgaagt acgtcggcgg cgaggccccg
     5821 acgctgcacc gactcggtgg ctcggactgg gcccagacca aggcccgggc ccgccgccac
     5881 gtcaaggaga tcgcggccga cctcatccgg ctctactccg tgcggatgtc gacggtcggg
     5941 catgccttcg ccccggacac gccctggcag cgtgagctcg aggacgcctt cgcctacgtc
     6001 gagacccccg atcagatctc cagcatcgac gaggtcaagg ctgacatgga gcgcgaggtc
     6061 cccatggacc ggctcatctg tggcgacgtc ggttacggca agaccgagat cgccgtccgg
     6121 gctgccttca aggccgtgca ggacggcaag caggtcgcca tcctcgtgcc gaccacgctg
     6181 ctggtccaac agcacctgca gaccttcacc gagcggtatg cgcagttccc cgtgacggtc
     6241 aaggcgctca gccgcttcca gtcgacgacc gaggccgggc gggtcgtcga cgggctggcc
     6301 gacgggtcgg tcgacgtcgt catcggcacc caccggttgc tcaccgacaa gatccgcttc
     6361 aaggacctcg gcctggtcgt cgtcgatgag gagcagcgct tcggcgtgga gcacaaggag
     6421 aagctcaaga ccttccggac ggccgtcgac gtgctggcga tgtcagccac ccccatcccg
     6481 cgcaccctcg agatggccgt gaccggcata cgcgaaatgt caactctcgc aacgcctccc
     6541 gaggagcggc acccggtcct caccttcgtc ggctcctacg acgagaagca gatcaccgcg
     6601 gcgttgcgcc gcgagctgct gcgcgagggc caggtgttct acgtccacaa caaggtgcag
     6661 tcgatcgaga aggccgccgc ccggctgcgc gagctcgtcc ccgaggcgcg catcgccacg
     6721 gcccacggca agatgaccga gggccggttg gagcaggttg tcctcgactt ctgggagaag
     6781 cggttcgacg tgctcgtgtg cacgacgatc gtcgagaccg gcctggacat ctccaacgcc
     6841 aacaccctca tcgtcgagcg cgccgacctg ctcggcctga gccagttgca ccagttgcgc
     6901 gggcgggtcg gtcgcggccg ggagcgcgcc tacgcctact tcctcttccc tccggagaag
     6961 ccgctcaccg agaccgcgca cgaccggctc gcgacgattg ccagccacac cgacctgggc
     7021 tcaggcatgc aggtggcgat gaaagacctc gagatccgtg gcgcgggcaa cctcctcggc
     7081 ggcgagcagt cggggcacat cgagggcgtc ggcttcgacc tctacgttcg cctcgtcggt
     7141 gaggcggtcg cggcatacaa gggggaggcg caggccccac ccgccgaggt gaagatcgag
     7201 ttgccggtcg acgcgcacct cccgcacgac tacgtccccg gcgagcggtt gcgcctggag
     7261 gcctaccaga agctcgcgtc cgtgcaggac gaggcgggcg tcgccgcgat cgaggaggag
     7321 ttgcgcgacc gctacggtat gccgcccgag ccggtcacca acctcatcga ggtggcgcgg
     7381 ctgcgtaccg tcgcccgcct ggccgggctg tcggacatct cggcgcaggg ggccttcgtg
     7441 cggttcggac cggtgccggc cgaagccatg cccgacagtc gccggctgcg cctacagcgg
     7501 ctctatccgg ggtcgatcgt caaggaccaa ctcggccatc tgctcatccc tcgcccgatg
     7561 accgcccgcg tcgggggcaa gccggtgcgc gacactgcgg tgctgcgctg ggccgccgac
     7621 gtgatccgtg cggtcttggt ggacaatgtc ggggacgccg cacgcgtcgc cacgaacgga
     7681 aaggacctgc gcccatgagc ccgcggaccc cgcgtcgcct cggaacgctc ccggcagccc
     7741 tcatcggact gctcgtcgcc accctgatcg ccgggtgcgg tgccgccgga cgcccgggca
     7801 cggctctcgt cgtcgacggc cagagctaca cgccgagcca gatcgtcgag acgaccgcgc
     7861 agatcaacga ggcgctcaag gccggcggcg aggccgccac cgaggcgcag atcgtcaacc
     7921 tcctcatgat ctcgccgttc gtcctcgccg aggcgacgag ccgcggtctc tgggggccgg
     7981 acaaccagta caactccttc atgactgcga tccacaaccc gacccccgtg acggtcgccg
     8041 tggtgcaggc cagcgcggcc tacgcctcgc tcgacgagga gggcaaggcc gccctcctca
     8101 aggacatcaa ggccgcctct atcgcggtcg acccgcgcta cggatcggtg gactactcga
     8161 ccggcttcct ggggatgccc acgcccgact ggatcgtccg gaccaccacc gcccccgaga
     8221 agtgaccgac cgcgggtgac gggcggccca ggcccgagcg ttccatccgg cgcggcgctg
     8281 ctggacctgg tcgcggtcat ggaccggttg cgctcgcccg gcggctgccc gtgggacgcc
     8341 gagcagaccc acgcctcgct cgtgccctac ctcgtcgagg agacctacga ggcggtcgag
     8401 gcgatcgagt cgggtgaacg cgaccacatg gccgaggaac tcggcgacgt cctgctgcag
     8461 gtcgtcttcc acgcgcgcgt tgcgcaggag gacgaggtcg ccccgttcga catcgacgat
     8521 gtcgccgccg ggatcgccgc caagttgcgc cgccgccacc cgcacgtctt cggggacgtc
     8581 gtcgctgaca cccccgaggc ggtcgccgcc aactggtcgg tgatcaaggc ggccgagaag
     8641 gctgccaagg ggtccggcgc cgcgccgcca ggcccgctgc acggcatacc ggcgggtatg
     8701 ccgcccctcg ccaaggccgc ggccatggtc gcccgcctcg accgggccgg gcgcggtgac
     8761 ctcgtggatg cggcggccgc cgggtccgac ccgggctccc gactgctggc cgtggtggcc
     8821 gacgtggtgc gccgacgtga tgatcctgac gctgtccttc gggccacgct gaggtccctg
     8881 agcgacgctg cgggataggg tgggcgcgac ggctgggtgc ggctgcgcag tgccgcgcgc
     8941 catcaacacc gggccgacct ttatccccat cccaacacgt tcggaggcac cgaagtggcc
     9001 agcatcgaag cagtaggcgc tcgcgagatc ctcgactcgc gcggcaaccc cacggtcgag
     9061 gtcgaggtgc tcctcgacga cggaaccgtg gcccgcgccg ccgtcccgtc gggtgcgtcg
     9121 actggtgcgt tcgaggccta cgagaagcgt gacggcgaca agggccgtta cctcggcaag
     9181 ggcgtcgaga aggccgtcga cgcggtcctc gacgagatca accccaagat catgggcttc
     9241 gacgccagcg agcagcggct catcgacgcc gagatgctcg ccatggacgg ctccaagaac
     9301 aaggagaacc tcggcgccaa cgccatcctc ggcgtctcgc tcgcggtcgc ccgtgcggca
     9361 gccgactcgg ccaacctccc gttgttccgc tacgtcggcg gccccaacgc gcacgtcctg
     9421 cccgtcccga tgatgaacat cctcaacggt ggctcgcacg ccgacaccaa cgtggacatc
     9481 caggagttca tgatcgctcc gatcggtgcg ccgaccttca aggaggccct gcgctggggc
     9541 gccgaggtct accacaccct caagaaggtc ctgcacgacc gtggcctgtc caccggcctg
     9601 ggtgacgagg gtggcttcgc ccccaacctc gagagcaacc gcgccgctct cgacgtcatc
     9661 gtcgaggcaa tcgagaaggc cggctacgtt ccgggcaagg acatcgcgct ggccctcgac
     9721 gtggcggcca ccgagttcta caaggacggc gcctaccagt tcgagggtgc cgccaagacg
     9781 tccgccgaga tgaccgccta ctacggcgag ctcgtcgcgg cctacccgct ggtgtcgatc
     9841 gaagacccga tgagcgaaga ggactgggac ggctggaagg ccatcaccgt cgccctcggc
     9901 gacaaggtcc agatcgtcgg cgacgacctg ttcgtcacca accccgagcg gctgcagcgc
     9961 ggcatcgaca cccagacggc caacgccctg ctcgtcaagg tcaaccagat cggctcgctc
    10021 accgagacgc tcgacgcggt cgacctggcc caccgcaacg gctaccgctg catgatgagc
    10081 caccgctccg gcgagaccga ggacacgacg atcgccgacc tcgccgtcgc gaccaactgc
    10141 ggtcagatca agaccggcgc cccggcccgg tccgagcgcg tggcgaagta caaccagctc
    10201 ctgcgcatcg aggaggagct ggacgacgcc gcggtgtatg ccggtgccgg cgccttcccg
    10261 cgtttcgctg ccaagggctg agccgttgag gggtcagact ggggggatgg ggacctccgg
    10321 ggcatgacca cgccagctcg acgcacgcgc gtgcgccccg ggacgacggc caccaccggc
    10381 cgtcgccccg gggccagccg ccctgcccct gcaccccgga cccgtgaggc gacccccgac
    10441 gacgatcgtc gcggcccggc ctggttgcgc tgggtcatcc tcggcgccat cgccgtcgtc
    10501 ctgctcgtca ccctcgtgcc gaccgcccga tccctggccc gacagcgggc cgagatcgac
    10561 gccctggcga cccaagtcgc ccagcaacag agccaagtgg cctcgctgca agccgagcag
    10621 gagcggtgga ccgaccccgc ctacgtcgag caacaggccc gcgaccggct gaagttcgtc
    10681 aaggtcggtg accgctccta caccgtcatc gacccgcagg aggccgcccg gccgcccgtc
    10741 tctggtgcct cggtcgccgc gccccaggcc aacggcgacg ccccctggta tggccagatc
    10801 tggcagtccg tgcaactggc cgaccgtccg accgccggca tgcttccggt caccgcgccg
    10861 ccgaccgccc cgtgacgccg ccgactgagc cgactgggcc gactgggccg accgagcctg
    10921 aagacctcgc cgcgatcgcc gggcagttgg gccgggagcc acggggcgtg ctcgggatcg
    10981 cccaccgctg cccgtgcggt cgtcccgacg tcgtccgcac cgccccgcgg ctgcccgacg
    11041 gcaccccgtt cccgacgacc tactacgcca cgtgtccccg gctgaccgcc gcgatcagca
    11101 ccctggagac ccaaggtctg atgcgcgaca tgcaggcccg gttggccgac gacgaggacc
    11161 tggcggccgc ctacgcgcgg gcgcacacgg catacctcgc cgatcgggcc accctcgcgt
    11221 ccggcggcga cgtccccgag atcgcgggtg tctccgcggg tgggatgccc gaccgggtca
    11281 agtgcctgca tgtcctcgtc gcacactcgc tggctgccgg ccccggggtc aacccgttcg
    11341 gcgacgaggc cctagcgttg ctggccccct ggtgggacgc cgggccgtgc gtgggagccg
    11401 ccgaggagtg acccggcgga gtccgggggt gccgtgtcgg gggagcgatg ggataatcgc
    11461 cgcatggtcc ccgccaccgc gcctcgcgcc acttccgccg ccggggaggt ccgagtcggg
    11521 gccatcgact gcgggaccaa ttcgctgcga ctgcttatcg ccgacatcga tccgcagacc
    11581 gggaagatgc ttgagctggt gcggcggatg gagatcgtcc ggctgggctg cggggtcgac
    11641 aagacggggg tgctcgcccc tgaagcgctg gcgcgcacgc tggcgatgac ccgcgactac
    11701 gcgacccaat gccgtgagta tgccgtccac cgcatccggt tcgtcgccac ctccgcctcc
    11761 cgcgacgcgc gcaacgccga cgagttcgtc gccggggtcc gtgcagcgtt cggggacctc
    11821 gacgtcgcac ccgaggtcgt cagcggccaa gaggaggctc ggctgtcctt cctcggggcc
    11881 accggtgacc tcatcgccgc gcagatggag gggccctatc tcgtcgtcga catcggtggc
    11941 ggctccacgg agttcgtccg gggcacccac acggtggagg ccgctcgctc ggtcgacatc
    12001 ggttgcgtgc ggctcaccga gcgccacctg cgcagcgacc cgccgacccc cgacgagatc
    12061 gccgcggcga tcgtcgacat cgaccgggcc atcgacctgg ccatcgagac ggtcagcttg
    12121 cgtgaggtgc gcaccctcgt cgggttggcc ggcagcgtaa cgaccgtggc ggcccagcac
    12181 ctcgacctgc gggactacga cccgggagcg atccacctgt cggtcgccac gcccccgcag
    12241 atccaggcgg cgtgcgagca actcatgggg atgacccggg cagagcgcgc ggccaagggc
    12301 ttcatgcacc ccggccgggt cgatgtcatc ggtgcggggg ccctggtctg gtcgcgggtc
    12361 atcgcccgcg tcatgcgcca gactggtctc ggccaggccc tgcccgtcgc tacctccgag
    12421 cacgacatcc tcgacggcat cgcgctgtcg ctcgtcggcc gctgagtccc gcgccgtgcc
    12481 tcaggcgttg ccgcatccgg tcacggggga cctgtcgggc tcgcctgcac cccctggctc
    12541 tgggtggcct ggggacccgg cgacgcggca tacccctcgg gctcgtgacg ccgagcaggt
    12601 ggtgagcctc gcgcggtcgg cctccgacct cgccaccctc gacgcgcgga tctcggtatg
    12661 ccgtgcctgc ccgcgcctgg tccagtggcg tgagcaggtg gcgggggaca agcgggcggc
    12721 gttcgccgat cagccctact gggggcgacc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12841 nnnnnnnnnn ccgacggtgt ccgagcgggc gacgtgcgcg ccgtggttgg accgcgacct
    12901 cgcgctcgct gcgccgaccg ttcgctcgat cctggcgctg ggcggtctgg cctgggacgc
    12961 gtcgctgggc gcggcccgcc ggctcggctg gcaggtcccg acccctaagc cgacgtttgg
    13021 gcacggcgcg atggcgcagt tgcgcgagcc gggcggacgc gtcgtgcggt tggtcggcag
    13081 ctatcacgtg agccagcaga acaccttcac cgggcggctc accgagtcga tgctggactc
    13141 ggcgctggcg ctcctcgcgt ggcccgatac cgtagccgag tgagcacaac ccccactccc
    13201 gtcctcgcgg tgaccgtcct cggagacgac cggcccggca tcgtcgccga ggtcaccgcc
    13261 gccgtcgccg ggttgggcgg caacctcgaa gactccacga tgacgttgct gcgcgggcac
    13321 ttcgcgatgg tcgtgttggt gcgcaccccc gcgtcggttg ctgacgtcga ggccgcgctg
    13381 gccgggatcg ccgcgggcgg ctcgctcacc gtcgacgtcc gcgagttgcg ggagtcgggt
    13441 atgccgcccg ctcccggccg cgcctggacc ctgcgggtcc acggggctga ccggcccggg
    13501 atcgtcgcct cgatcactga ggtgctggcc gctcacggcg gcaacatcgt cgacctgggc
    13561 acccggttgg ggtccgacct ctacgtgctc atcgccgagg tcgagttgcc ggaagccgcg
    13621 tccggcgagg ccgtcgcggc cgacctgcgc gcagccgccg ctgcggtcgg cgtcaccgtg
    13681 gccttcgagc cggtggacga cgaggtgctg tgagttcgtt cgtcatgccc tcgctccctg
    13741 agggtcgagt cctcgatgtc gtgcgcgccc ccgcgccggt gttggccacc cctggggctg
    13801 atgtcgatcc ggccgcgccg tcggtcatcc aactggccgc cgacctgttg gccacccaag
    13861 cggtgtcgcc agggtgtgtg gggctggccg ctcaacaggt gggcgtggcc tggcggatgt
    13921 tctcggtcga cgtcaccggg caccccaaga cccggacgtg tcacggcgag ttcgtcctgt
    13981 gcaacgccgt cgtcgagtcg gccagtcgca aggagaaggg ccgcgagggg tgcatgtcgg
    14041 tgcccgactt caccggcgac gtggcccggg cgcggcggtt ggtggtgcgc gggcagttgc
    14101 cggtcacggg ggagtgggtg accctggaga ccgatgcctt cgaggccgtc tgcctgcagc
    14161 acgagatcga tcacaccaac ggcctggtct tcctcgaccg agtcgccggc ccccaagccg
    14221 tccaccccag acgcacctac ctctaacgcg cgcccggccc tcttagcccc cccccccccc
    14281 aacccccccc cccccccccc
//
LOCUS       DAOMZY010000037        14189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_3912_length_14189_cov_6.039196, whole genome shotgun sequence.
ACCESSION   DAOMZY010000037 DAOMZY010000000
VERSION     DAOMZY010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 14189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14189
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3912_length_14189_cov_6.039196"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PLK64_03600"
     CDS             <1..247
                     /locus_tag="PLK64_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPV78855.1"
                     /translation="GLFPDLTIVVDVPAAVGRARRGSVHDRIESEGDDFHERVRQHFL
                     DLAAAAPQRYLVLDGTADPADLHAQVVARLRAEGVAL"
     gene            244..1395
                     /locus_tag="PLK64_03605"
     CDS             244..1395
                     /locus_tag="PLK64_03605"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HPV78856.1"
                     /translation="MSVWDEVVGQSQTVRTLSHAATHPDAMTHAWLLTGPPGSGRSTA
                     ARAFAAALQCPNHGCGTCRECRTAVEGTHADVRIVATEGLSIKVEDTRALVQEAGLRP
                     SVGAWRVIVIEDADRLTERAADALLKALEEPTPRTVWILCAPSLEDVIVTIRSRCRHV
                     RLRTPPVAAVAELLTRRDGIPEPMARYAARAAQCHVGKARRLARDEGARNRRHEIISL
                     PLRIRGVGDAVGAAADLASIADAEAGASSSSRDAAERTRLLETLGADPEARTQPPHVR
                     AQLAALEKEQKARATRFGRDVVDRSLVDLLSMYRDALVVRAGATVELVNAEALDQVRA
                     LAAAMSAERLLACMDAVGEARERINLNVPPLLALEAMAVSLQVPTPSTR"
     gene            1488..3113
                     /locus_tag="PLK64_03610"
     CDS             1488..3113
                     /locus_tag="PLK64_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPV78857.1"
                     /translation="MRLPTARPRSTKVDRSRVDRRLALVASGVVAVLSLSACLVLPFL
                     PNDPATPSGGSTTVPLSTADASAVRQPPAGTTGLDRFYAQQLAWSSCQGGECATLEVP
                     IDYAQPQGATIKLALLRVKAKSSNGRLGSLVVNPGGPGGSGVEYASYANQIVGAPVRA
                     AYDIVGFDPRGVGDSAPIDCLDDAGLDAFLGMDPTPDDLAEEQAFVKGAKALADGCKA
                     RGGALLAHVSTVDAAKDMDILRAALGEAKLHYLGKSYGTFLGSTYAGLFPAKVGRFVL
                     DGVVPPDLTSSELSQGQARGFEEATRAYVANCIKRSSCPVGSTVDEGMQWLRDFLKGL
                     DGQPIRVTTDAKITELNEAWAAIGLANAMYSQQAWPSLTSALIAAQNGDGDELMDLAN
                     QYADRTSGGTYAGNIMEVIYAVNCLDRPDTADLTVIEKNADAFAQVAPTWGRFLAWGS
                     VPCGVWPIQTGNTPAKITAAGSGPIVIVGTTRDPATIYEWSVRLRGDLANSVLLTFDG
                     DGHTAYTRSNSCIDKPIDDYYVKGIVPTDGLKC"
     gene            complement(3165..4448)
                     /locus_tag="PLK64_03615"
     CDS             complement(3165..4448)
                     /locus_tag="PLK64_03615"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPV78858.1"
                     /translation="MTLRGPLSLGAALAAGLSLTLSSCTGAGAPATLAGGSPTASATV
                     VAKPAASNAGTPAPAPGGTTPTPAPAPATPAKDGSTVTIALTGDVLLHSGVTDQAARD
                     GAANPGQASHGLDYRPLLAGAKPFVEGADLAICNLETPVAPPGGPYTSYPVFSVPPEI
                     LPALVDTGFDACTTASNHTVDKGVPGIQRTLDAMDAAGLKHAGSNRSAEEQSGTTMLD
                     AHGVKVALLAYAYGLNGFRLPADKAWAVDVIDTATILADARAARAAGAQIVLVALHAG
                     TEYQILPTAQQRDVATALAASPDIDLIYGHHAHVVQPFDKIGDTWVAYGLGNMIAHQH
                     RLPKLTMAQHEVMASFTFAQGSDGRWTVTRAEARPAAMSNLNEPLRYVDLATLLADPA
                     VSEARKAAYRPFYDRAVAALTKLGADGKGLVIAGR"
     gene            4632..4704
                     /locus_tag="PLK64_03620"
     tRNA            4632..4704
                     /locus_tag="PLK64_03620"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4665..4667,aa:Thr,seq:cgt)
     gene            complement(4837..5232)
                     /locus_tag="PLK64_03625"
     CDS             complement(4837..5232)
                     /locus_tag="PLK64_03625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV78859.1"
                     /translation="MRNQDSGASRTFVTTCDGLVVGYYALAASSVAQVGAPTGLRRNM
                     PDPIPVILLGRLAVDRRHAGRGVGASLLRDAALRVATTAGIVGVKAMLVHAIDDGAVA
                     FYLHHGLQPFPGDPTTLFVPVAAIRPPGM"
     gene            complement(5331..5603)
                     /locus_tag="PLK64_03630"
     CDS             complement(5331..5603)
                     /locus_tag="PLK64_03630"
                     /inference="COORDINATES: protein motif:HMM:NF020265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HPV78860.1"
                     /translation="MSTTAKDERLALRLTRSQRKALEAAATATGSTLTEFSVAAILAR
                     AEDVLGSRAVFEVDEQVWSEFVNALDQPVTSDRFAALIARPPVWDE"
     gene            5739..6758
                     /locus_tag="PLK64_03635"
     CDS             5739..6758
                     /locus_tag="PLK64_03635"
                     /EC_number="3.5.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008660 -
                     1-aminocyclopropane-1-carboxylate deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0042217 - 1-aminocyclopropane-1-carboxylate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-aminocyclopropane-1-carboxylate deaminase"
                     /protein_id="HPV78861.1"
                     /translation="MKLHEFPRHPLTFGPSPVHPLRRLTQHLSGAQIWAKREDVSSGL
                     AFGGNKVRKLEYIVPDVLATGADTLVSIGGYQSNHTRQVAAVAAYLGLKCRLVQEKWV
                     PWDDPVNDKVGNILLSRMMGADSRLDPHGFDIGIRDSWKDALREVEASGGTPYPIPAG
                     ASEHRLGGLGFANWAFEVAEQEKALGVFFDTIIVCTVTGSTHAGMIAGFAALEELTGV
                     RRRVIGIDASATLEQTRDQVGRIARHTSELIELGRDLRDDEITVLEGWAGDLYGVPVD
                     STMEAIRLGAQLEAMITDPVYEGKSLAGLIDLVRSGDIPATSTVLYAHLGGQPAINAY
                     HRLWS"
     gene            6939..8273
                     /locus_tag="PLK64_03640"
     CDS             6939..8273
                     /locus_tag="PLK64_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4921 family protein"
                     /protein_id="HPV78862.1"
                     /translation="MDTDHLWPEPLRRMPDGTVKQINPYSGTSVWTVPGRGNRPIASG
                     LPAPGPIDPAMAGRHCAFCEGRYFDTPPEKSRLVADPDGWHLLRGLTANRIEDSVAQF
                     RRIPNLFEIVAYDYWRLNYGFEPGSEARERHDHYLSTDLGRKHVDQMIRTRAMAGGMS
                     EAQWAALTPQDKAQRAMSFFAGTHDVIVARRHFVDGASTTDQLAASGSLTPDEHAAYL
                     RFTVDALRSLYDANRYARYVAVFQNWLRPAGASFDHLHKQLVAVDERSVRTEHELRRL
                     RDNLNLYNEFAVDYAARQNLIVAENPYAVAFAGFGHRYPSLEVYSKSEFARPWEQPSD
                     DLRGFSDLLHACHAATGAAVPSNEEWHYQPPDVDLPMPLRTVLKWRVSTLAGFEGGTK
                     IYLNTIDPWHLRDRVVGALFELREQGRIAPDIRIATECACRPNSLKYNPLLH"
     gene            8555..10009
                     /locus_tag="PLK64_03645"
     CDS             8555..10009
                     /locus_tag="PLK64_03645"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HPV78863.1"
                     /translation="MAKSARASRRGLRWAIAGSVAVALVASTATGASADPTPVVPSAE
                     QVSAAAAAAGSAEAQVAALDAELAVARATLQEQQERAGAAAEDYNRAAEELDEATSAH
                     TTATAKATAAAQASEEATLALSRYAAEVFQSGGGLGQLDVFFGGGGPAQTLDRAAGLE
                     AVGEERARVMRDAESARHLARTLAEAAAEAQQRQEQAAQTAHAARDIAQRDNDAAAAA
                     AATAAAHQEQALGQLATLRNTSVELERQRQDGLQAIEQARIAEEARRKAEEAARQAAA
                     QAARLAAAEAARLAEADRQAAAAAAEAARKAAEEAAKKATSTPSPTPTPKPSTTPTPK
                     PSTTLTPTPTPTKSTTPPPTPPPPATNVPAPKAGVDAVIAFARAQLGEPYVWAAAGPN
                     SWDCSGLTMVAWAQAGVRLTHYTGSQYKQTARVPVDQLKPGDLVFFGPSVAGIHHVGL
                     FIGNGQMIHAPYTGTVVKISTIWRPDLIPYGGRP"
     gene            complement(10033..10554)
                     /locus_tag="PLK64_03650"
     CDS             complement(10033..10554)
                     /locus_tag="PLK64_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HPV78864.1"
                     /translation="MEFDVTIEIPKGHRNKYEVDHATGRIRLDRMLFTSMAYPGDYGY
                     IEDTLGEDGDPLDAVILLDEPTFPGCVVRARAIGVFQMTDEAGGDDKILCIPAGDPRQ
                     AHITELDQINKFETAAIGHFFDTYKDLEPEKSVDASRWFGRVVAERVIIEAIERARAA
                     GHTTARWRPTESH"
     gene            10635..12050
                     /gene="dacB"
                     /locus_tag="PLK64_03655"
     CDS             10635..12050
                     /gene="dacB"
                     /locus_tag="PLK64_03655"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HPV78865.1"
                     /translation="MRRVTAVALAGLLAIGGYGTADVLDLVPGLITLAPEAAAYPTSP
                     SPTSSAGATAHLPLPSAAPSGMPVAPVDTTAPLPSVAGIKAAVAPALADPDLKGAAVV
                     VRDALTGADLLDVSGSEPRIPASTLKLIAALAVQQVFPDGTTLVTRVVQGASPDTVVL
                     VAGGDMFLAPDKGDPAATVGRAGLGDLADSVATALRAKAITQVTVQVDTSYAPGPQVA
                     AGWQPAFRALGIVGPVAMLGLSTQWAQSGKPGPADPVAEVVAAFAARLTERGITAVAG
                     GRASGAGVSLGEVHSAPVTDVLGLALERSDNTVTESLARQAAFRVGRPSDFEGTAAYL
                     REALTLAGIDPRGMSVHDASGLSRTSHVPARVLADVLNLGVKGTVPGFTQALRHLPVA
                     GLTGTLGTRFTAAPQAPAAGLVRAKTGSLNGVNSLAGTVVTADGRMLVFAIMQEGPVG
                     TTDARAALDRFGAALATCGCR"
     gene            12070..13161
                     /locus_tag="PLK64_03660"
     CDS             12070..13161
                     /locus_tag="PLK64_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HPV78866.1"
                     /translation="MSDQPVDRPSDHPGDAPGPFIDWDFARVTAARLASPGPKVNAGE
                     AAGVVAGLRQAALAAHDPVAETSRLTSPPGSAPVLIVDRAGWASANIDSMRELIDPVV
                     AKMAAGMPSQSGEGMAAMGSKITGAETGALLAFFSSKILGQYDLAPSGQPRLLLVAPN
                     IVSAEREMGVSPRDFRLWVAMHEETHRVQFTAVPWLREHMIERSRTLALELAPTPSEF
                     AERIQQIAQGLPGAFRPGSTGLAEVFLTPEQKGKIAEITAVMSLLEGHADVIMDDVGP
                     SVIPTVAEIREKFTQRRAGLGQLDRLIRRLLGLEAKMRQYADGAVFVRAVVDRVGIDG
                     FNAVWTSPQTLPTAAEMADPQAWVARVHG"
     gene            13178..>14189
                     /gene="tilS"
                     /locus_tag="PLK64_03665"
     CDS             13178..>14189
                     /gene="tilS"
                     /locus_tag="PLK64_03665"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPV78867.1"
                     /translation="MPGPPAAVAAVRLAVRGVLADLPTWSTVLVACSGGADSLALAAA
                     LAFEAPGAGLRAGAVIVDHGLQEGSADVAAQAAQACRGLGLDPVDVVRVAVPDSPTGP
                     EGAARTARYAALADAATRHTAALVLLGHTRDDQAEQVLLGLARGSGARSLSGMPPSRG
                     PYARPLLGVTRAQTEAACREAGLEPWRDPHNSDPTYARVRARAALGELNAVLGPGLGA
                     ALARSADLLRDDADLLDELAAQARADVDLDPAGERVVAAQAGAGDVPAVRVAVVGLAA
                     LPPALRRRVWRIVAAEAGARALSAAHVALLDGLVLRWHGQGAIDLPGGVVALRVGGTI
                     QMG"
BASE COUNT         2051 a         5035 c         5019 g         2084 t
ORIGIN      
        1 cgggttgttc cccgacctga cgatcgttgt cgacgtgccg gcagccgtgg gccgagcccg
       61 ccgcggatcc gtccacgacc gcatcgagtc cgaaggcgac gatttccacg agcgggtccg
      121 ccagcacttc ctggacctgg ccgcggccgc cccgcagcgc tacctcgtgt tggacggcac
      181 cgctgatccg gccgacctgc atgcccaggt cgtcgcccgc ctgcgcgccg agggggtcgc
      241 cctgtgagcg tctgggatga ggtcgtcggt cagtcccaaa ccgtccggac gctcagccac
      301 gccgccacgc accccgacgc gatgacccac gcctggctgc tcaccggccc acccggctcc
      361 ggccgatcga ccgcggcccg ggccttcgcg gcggcgctgc agtgccccaa ccatggctgc
      421 ggcacctgcc gggagtgccg caccgccgtc gagggcaccc acgccgacgt gcggatcgtc
      481 gccaccgagg gcctgtcgat caaggtcgag gacacccgcg ccctggtcca ggaggccggg
      541 ctgcgcccgt cggtcggggc ctggcgggtc atcgtcatcg aggacgccga ccgcctcacc
      601 gaacgcgccg ccgacgccct cctcaaggcc ctcgaagagc ccacgccccg cacggtctgg
      661 atcctctgcg ccccctccct cgaagacgtc atcgtcacga tccggtcgcg gtgccgccac
      721 gtccgcctgc gcaccccgcc cgtcgcggcg gtggccgagt tgctgacccg gcgggacggc
      781 atacccgagc cgatggccag gtatgccgct cgggcggccc agtgccatgt ggggaaggcg
      841 cgccgcctgg cccgcgacga gggggcgcgt aaccggcgcc acgagatcat cagcctcccg
      901 ctgcgcatcc ggggggtggg cgacgcggtc ggggccgccg ccgacctcgc gagcatcgcc
      961 gacgcagagg caggagcgtc gtcgtccagc cgggacgccg ccgagcgcac ccggctgctg
     1021 gagactctcg gggccgaccc ggaggcccgc acccaacctc cccatgtccg ggcccaactg
     1081 gcggccctgg agaaggagca gaaggcccgc gcgacccgct tcgggcgcga cgtcgtcgac
     1141 cggtcgcttg tcgacctgct gtcgatgtac cgcgacgcgc tcgtcgtccg tgcgggggca
     1201 accgtcgagc tcgtcaacgc cgaagcgctc gaccaggtgc gagcgctggc cgccgcgatg
     1261 agcgccgagc ggctactcgc ctgcatggac gcggtcggcg aggcgcgcga acggatcaac
     1321 ctcaacgtcc ccccgctgct cgcgctggaa gccatggcgg tctcactcca ggtcccgacc
     1381 ccctccacca ggtaaccttc cgccaagacg gcagatgtcc gggtcggttc gtccggacga
     1441 ttgggtatgc cgcccgcttg agtggacgag aaggagcacc accggtcatg agactcccga
     1501 ccgcacggcc ccgcagcacg aaggtggacc gcagccgggt cgaccggcgg ctggccctcg
     1561 tcgcatcggg cgtggtggcc gtgctgtcgc tctcggcgtg cttggtgctg ccgttcctgc
     1621 cgaatgaccc ggcgaccccg tcgggcggaa gcacgaccgt gcccttgtcg acagccgacg
     1681 cgagcgcggt ccggcaaccg cctgcgggga cgaccggcct cgaccgcttc tatgcccagc
     1741 aactggcgtg gagctcctgt caaggcggcg agtgcgcgac cctcgaggta ccgatcgact
     1801 acgcccaacc gcagggggcg acgatcaaac tcgcgctgct gcgggtcaag gccaagtcct
     1861 ccaacgggcg cctcgggtcg ttggtcgtca accctggcgg gccgggcggt tccggggtgg
     1921 agtacgccag ctacgccaac cagatcgtcg gcgcgccggt gcgcgcggca tacgacattg
     1981 tcggtttcga cccgcgcggg gtgggcgatt cggctccgat cgactgcctc gacgacgcgg
     2041 gtctggacgc cttcctcggg atggacccga cgccggacga cctggccgag gagcaggctt
     2101 tcgtcaaggg ggccaaggcg ctcgccgacg ggtgcaaggc gcgcggcggc gcgctgctcg
     2161 cgcacgtctc gacggtcgat gcggccaagg acatggacat cctgcgggca gcgctcggcg
     2221 aggcgaagct gcactacctg ggcaagtcct acggcacctt cctcggatcg acgtatgccg
     2281 ggctgttccc ggccaaggtc ggccgcttcg tcctcgacgg cgtggtgccg ccggacctca
     2341 cctcctcgga gttgtcccag ggccaggccc gcggcttcga ggaggcgact cgcgcgtatg
     2401 tcgccaactg catcaagcgc agctcctgtc cggtcggatc gaccgtcgat gagggcatgc
     2461 agtggttgcg tgacttcctc aaggggctgg atgggcagcc gatccgggtg accaccgatg
     2521 cgaagatcac cgagctcaac gaggcctggg ccgccatcgg gctggccaac gcgatgtact
     2581 cccagcaggc ctggccctcg ctcacgtccg ccctgatcgc cgcacagaac ggtgacggcg
     2641 acgaactcat ggacctggcc aaccagtacg ccgatcgcac ctcgggcggg acctacgccg
     2701 ggaacatcat ggaagtcatc tacgcggtca actgcctgga ccggcccgac accgccgatc
     2761 tgacggtcat cgagaagaac gccgatgcct tcgcccaggt cgcgccgacg tggggtcggt
     2821 tcttggcgtg gggttcggtg ccctgtgggg tttggccgat ccagaccggc aacacgccgg
     2881 ccaagatcac cgcagcgggc agcggtccga tcgtcatcgt cggcaccacc cgggacccgg
     2941 cgacgatcta cgaatggtcg gtccggttgc gcggtgacct cgccaactcg gtgctgctca
     3001 ccttcgacgg tgacgggcac acggcataca cccggtccaa ctcgtgcatc gacaagccga
     3061 tcgacgacta ctacgtcaag gggatcgtgc cgaccgacgg cctcaagtgc tgaggtatgc
     3121 cgcgcggttc gctcgcgcgt cgggtcgcgg gtgcggtggc gcggtcagcg gccggcgatg
     3181 accaggccct tgccgtcggc tcccagtttg gtgagagcgg cgacggcgcg gtcatagaac
     3241 ggccggtatg ccgccttgcg cgcctcgctc acggcggggt cggccagcag ggtggccagg
     3301 tcgacgtaac gcaacggttc gttgaggttg ctcatcgcgg ccggccgggc ctcggccctg
     3361 gtgacggtcc agcgcccgtc gctgccctgg gcaaaggtga acgaagccat gacctcgtgc
     3421 tgggccatgg tgagcttggg gagtcggtgc tggtgggcga tcatgttgcc caagccgtag
     3481 gccacccagg tgtcgccgat cttgtcgaac ggttgcacga cgtgggcgtg gtggccatag
     3541 atgaggtcga tgtccggtga cgccgccaac gcggtggcga cgtcgcgctg ctgggccgtg
     3601 gggaggatct gatactccgt cccggcgtgc agggcgacca ggacgatctg ggcaccggcc
     3661 gcgcgtgccg cacgagcgtc ggcaaggatc gtggcggtgt cgatgacatc gacggcccag
     3721 gccttgtcgg cggggagtcg gaaaccgttg aggccgtaag cgtaggcgag cagcgcgacc
     3781 ttgaccccgt gggcgtcgag catcgtcgtg ccggactgct cctcggcgct gcggttactg
     3841 ccggcgtgct tgaggccagc ggcatccatc gcgtccaggg tgcgctgaat gccggggaca
     3901 cccttgtcga ccgtgtggtt ggacgcggtc gtgcaggcgt cgaacccggt gtcgacgagc
     3961 gccgggagga tctccggcgg gaccgagaag acggggtaac tggtgtaggg accacccggc
     4021 ggtgcaacgg gcgtctcgag gttgcagatc gccaggtcag caccttcgac gaacggcttg
     4081 gccccggcga gcagcggccg gtagtccaac ccgtggctgg cctggcccgg gttcgccgcg
     4141 ccgtccctgg ctgcctggtc ggtgacccct gagtgcagca ggacgtcgcc ggtgagggcg
     4201 atggtcacgg tcgagccgtc cttggccggt gtggctgggg ctggtgcggg cgtcggtgtg
     4261 gttccgcccg gtgcgggggc cggcgtgccc gcgttcgagg cggccggctt cgcgacgacg
     4321 gtcgccgagg cggtggggga ccctccagcc agggtcgccg gcgctcctgc tccggtgcag
     4381 gaggacaggg tgagggacag gccggcggca agcgccgcgc cgagggagag agggccccgt
     4441 aaggtcacgg atccgagact aagccacgct cgcggcatac cgggggcttc cgggtgtgac
     4501 acgaaccggg ggtgacgcgc cccgccagag gcctgccccg ggaggggtat gccgcgcgcc
     4561 tcaggcaacc ggtttcgtgg ggtggcgccg gtgtccgtat actcggtgcc cgcgcctcgg
     4621 ctggggcgca cgccgcctta gctcagtcgg cagagcgatt cactcgtaat gaataggtct
     4681 ggggttcgat tccccaaggc ggctcagtga acgccctccg ggctcacccc ggggggcgtt
     4741 tctgctgccc gccccgctcg ccccggcttg cgtcatgcca accgcgacgt atgccgcgcg
     4801 tctggcatga cgcaactcgc tcgcctcgcg gccggctcac atgcctggag ggcggatcgc
     4861 cgcaacgggg acgaacaggg tcgtcgggtc gccggggaac ggctgcagac cgtggtggag
     4921 gtagaacgcg acggccccgt catcgatcgc gtggacgagc attgccttga ccccgacgat
     4981 gccggccgtt gtcgccaccc ggagagcagc gtcgcgcaac agtgaggcac cgactccgcg
     5041 gccagcatgt cgccggtcga ccgccaagcg gccgaggagg atgacgggga tcgggtcggg
     5101 catgttgcgt cgcaggccgg tgggggcccc aacctgcgca acggaggatg cggccagggc
     5161 gtagtagccc acgacgagcc cgtcgcaggt ggtgacgaag gttcgggagg cgccgctgtc
     5221 ctggttacgc acggctcgac tgaccagcca gcgattcagt gcgtcattcc cgctgtcgaa
     5281 gccggccagg tcgtgggttg gtcgcagggg ctcaggctgc gtgtagcgac tcactcgtcc
     5341 cacacgggcg gtcgggcgat gagggcggcg aagcgatctg aggtcaccgg ttggtcgagc
     5401 gcgttgacga actcgctcca gacctgctcg tcgacctcga agaccgcgcg cgagccaagg
     5461 acgtcctccg cccgcgccag gatggcggcg acgctgaact cggtgagggt gctcccggtg
     5521 gcggtggccg ctgcttcgag tgccttgcgt tgcgagcgag tcaggcgcag ggcgaggcgt
     5581 tcgtccttgg cagtggtgct catcggccca gtgtacgcct ctaaggcgta cggccgagtt
     5641 cccggattcc ccaacctcaa gcgcgatata ttgcagcacc agacgcgccc ctggcccccg
     5701 aaacgagcgg tcggtcgctc cactcccgag gagtcgtcat gaagctgcac gagttcccgc
     5761 gccacccgct caccttcggt cccagccccg tgcatccgct gcgccgcctg acccaacacc
     5821 tcagcggcgc ccagatctgg gccaagcgcg aggacgtcag cagcggcctg gccttcggcg
     5881 gcaacaaggt ccgcaaactc gaatacatcg tccccgacgt gctggccacc ggcgccgaca
     5941 ccctggtctc gatcggcggc taccagtcca accacacccg ccaggtcgcg gcggtcgcgg
     6001 catacctcgg cctgaagtgc cgcctcgtcc aggagaagtg ggtcccctgg gacgacccgg
     6061 tgaacgacaa ggtcggcaac atcctgctct cgcggatgat gggcgccgac tcgcgcctgg
     6121 acccgcatgg cttcgacatc ggcattcgcg actcgtggaa ggacgccctc cgcgaggtcg
     6181 aggccagcgg cggcaccccc tacccgatcc cggccggcgc gtctgaacac cggctcggtg
     6241 ggctcggatt cgccaattgg gccttcgagg tcgccgagca ggagaaggcc cttggcgtct
     6301 tcttcgacac gatcatcgtc tgcacggtca ccggctcgac ccacgccggg atgatcgctg
     6361 ggttcgccgc gttggaggag ctcaccggcg tccggcgccg ggtcatcggc atcgacgcct
     6421 cggcgaccct cgagcagacc cgcgaccagg tcggccggat cgcgcggcat acctccgagc
     6481 tcatcgagct gggacgcgac ctgcgcgatg acgagatcac cgtcctggag gggtgggcgg
     6541 gcgacctcta cggcgtgccg gtcgactcga cgatggaggc gatccgcctg ggcgcccaac
     6601 ttgaggcgat gatcaccgac ccggtctacg agggcaagtc gctcgccggc ctcatcgacc
     6661 tggtccgcag cggcgacatc cccgcgacct ccaccgtgct ctacgcccac ctcggcggcc
     6721 agccggcgat caacgcctac caccgcctct ggtcctgagc gtccgggggc gcccccgcgc
     6781 ggaacacgcc gaatgccatt ggtcgggggc gggcggcata cccgtgctca gtcgcggcgc
     6841 gcggcccgac cattggtatg ccgtgtgcgc gcggcacccg cgggtcggga ctgagggccc
     6901 gtgagacctg tcgcctcgta gctactgtgg ccgatgtgat ggacaccgac cacctctggc
     6961 cggagccgct gcgtcggatg cccgacggga cggtcaagca gatcaacccc tactcgggga
     7021 cctcggtctg gaccgtgccg gggcgcggca accggccgat cgcttcgggg ctgcctgcgc
     7081 cggggccgat cgaccccgcg atggcggggc ggcactgcgc gttctgcgag ggccgctact
     7141 tcgacacgcc gccggagaag tcacggctgg tcgcggaccc cgatgggtgg cacctgctgc
     7201 gggggctgac cgccaaccgg atcgaggact cggtcgcgca attccgccgg atccccaacc
     7261 tgttcgagat cgtcgcctat gactactggc ggctcaacta cgggttcgag ccaggcagcg
     7321 aggcgcgcga gcggcacgac cactacctct cgaccgactt ggggcgcaag cacgtcgatc
     7381 agatgatccg cacgcgggcc atggcggggg gcatgagcga ggcgcaatgg gcggcgctga
     7441 cgccccagga caaggcccag cgggccatgt cgttcttcgc cgggacgcat gacgtcatcg
     7501 tcgcgcgccg gcatttcgtc gacggagcga gcacgaccga tcaactcgcc gcctcggggt
     7561 cgctcacccc tgacgagcac gcggcatacc tgcgcttcac ggtcgacgcg ctgcgctcgc
     7621 tctacgacgc gaaccgctat gcgcgctacg tcgcggtctt ccagaactgg ctgcggcccg
     7681 cgggcgcgtc cttcgaccac ctgcacaagc agttggtcgc ggtcgacgag cgcagcgtgc
     7741 ggaccgaaca cgagttgcgc cgcctgcggg acaacctcaa cctctacaac gagttcgcgg
     7801 tcgactacgc ggcccggcag aacctcatcg ttgcggagaa tccgtatgcc gtggccttcg
     7861 ccggcttcgg ccaccggtat ccgtcgctgg aggtctacag caagagcgag ttcgcgcggc
     7921 cgtgggagca gccctccgac gacctgcgcg gcttctccga cctcctgcac gcctgccatg
     7981 cggcgacggg agcggcggtg cccagcaacg aggagtggca ctaccagccg ccggatgtcg
     8041 acctgccgat gcccctgcgg acggtgctca agtggcgggt ctcgacgctc gcgggcttcg
     8101 agggcgggac caagatctat ctcaacacga tcgacccctg gcatctgcgc gaccgggtgg
     8161 tcggtgccct gttcgagttg cgggagcagg gaaggatcgc cccggacatc cggatcgcca
     8221 ccgagtgcgc ctgccggccg aactccctga agtacaaccc gctgctgcac tgagggcctg
     8281 gccggcgtcc gaccggccac gcaccccgcc tttcgttacc atatcgttat ctcattcgaa
     8341 caggcgagcg aattcgctcg ataacctgcg aaaaaacagc cgtgtgattc gaccaatcac
     8401 acggctgtca ttcttgaatt acggcgtgtc gcaacggtat tggagtgatc gctgtttcgc
     8461 gtgtgacgaa tgggttctac gctcggcaac ggcctggggg agagggtcgt aggcaattcg
     8521 ggtgctcgtc aacggcagcg aaggagcgtg tggcgtggcg aagtcagcgc gcgcgtcgcg
     8581 acgcgggctg cgctgggcca tcgctgggag cgtggctgtc gctctggtcg cgagcaccgc
     8641 gaccggagca tcggcagacc cgacaccggt cgtgccgtcg gctgagcagg tctcggccgc
     8701 cgcggccgcc gctggttcgg cggaggctca ggtcgccgca ctcgacgctg agttggccgt
     8761 cgcccgagcc accctgcagg agcagcaaga gcgggcagga gcggccgcgg aggactacaa
     8821 ccgcgccgcc gaagaactcg atgaggccac gtcggcgcat acgacggcca ccgccaaggc
     8881 gaccgccgcc gcccaggcct ccgaggaggc gaccctcgcc ctgtcccggt atgccgccga
     8941 ggtgttccaa agcggtggcg gactcggcca actggacgtc ttcttcggag ggggcgggcc
     9001 cgctcagacg ctcgacaggg ccgctggcct tgaagccgtc ggcgaagagc gcgctcgggt
     9061 catgcgcgac gccgaatccg cacgccacct ggcccgcacg ctcgcggaag ccgcggccga
     9121 ggcgcagcag cgccaggagc aggccgcgca gacggcccac gcagcacgcg acatcgccca
     9181 acgtgacaac gacgcggccg cggcagcagc ggcgaccgcg gccgcccatc aggagcaggc
     9241 gctgggccaa ctagcgacgt tgcgcaacac gtccgtcgag cttgaacgcc agcgtcagga
     9301 cggcctgcag gcgatcgagc aggcgaggat cgctgaggag gcccgacgca aggccgagga
     9361 agccgcccgc caggccgcag cgcaggctgc tcgcctcgcg gcggcagagg ctgcccggct
     9421 ggccgaggcg gatcgccagg ccgcggcagc agcggctgag gccgcccgca aggcagcgga
     9481 ggaggcggcc aagaaggcga cctcgacccc aagcccgacc ccgacgccca agccgtcgac
     9541 gaccccgacg cccaagccgt cgacgaccct gactcccacg ccgaccccga cgaagtccac
     9601 cacgcccccg ccgaccccgc cgccgccggc aacgaacgtg ccggcgccga aggccggcgt
     9661 ggatgccgtg atcgccttcg cccgcgcaca actcggcgag ccgtatgtct gggctgcggc
     9721 aggccccaat tcgtgggact gctcaggcct gacgatggtc gcctgggcgc aggcgggagt
     9781 gcggctcacg cactacaccg ggagccagta caagcagacc gcccgcgtgc ccgtcgacca
     9841 actcaagccg ggtgacctgg tcttcttcgg ccccagcgtc gcgggcatcc atcatgtggg
     9901 gctcttcatc ggcaacggcc agatgatcca cgcgccctac acggggaccg tcgtcaagat
     9961 ctccacgatc tggcgccccg acttgatccc ctacggcggc cgtccctaac cgggccctcg
    10021 tgcagcgagg tctcagtggc tctcggtggg acgccagcga gcagtcgtgt ggccggcagc
    10081 ccgagcccgc tcaatcgcct cgatgatgac ccgctccgcc acgacgcgac cgaaccagcg
    10141 cgaggcgtcg accgacttct ccggctcgag gtccttgtac gtgtcgaaga agtgcccgat
    10201 cgccgcggtc tcgaacttgt tgatctggtc gagctcggtg atgtgcgcct ggcgcgggtc
    10261 accggccggg atgcacagga tcttgtcgtc gccgccggcc tcgtcggtca tctggaagac
    10321 gccgatcgcg cgggcccgca cgacacaccc ggggaaggtc ggctcgtcga gcaggatcac
    10381 cgcgtcgagc gggtcgccgt cttcgccgag ggtgtcctcg atgtagccgt agtcacccgg
    10441 gtaggccatc gaggtgaaca gcattcggtc gagccggatc cggcccgtcg catggtcgac
    10501 ctcatacttg ttgcggtgtc ccttggggat ctcgatggtg acgtcaaact ccacgtccgt
    10561 gcccctcttc gctggtctcg ggtgacctac gcttcggttg tcgttggtca gttcacagac
    10621 tagggggtga gaccttgcgt cgcgtcacgg cggtcgcctt ggccggcctc ctcgccatcg
    10681 ggggctacgg cacggccgat gtcctggacc tcgtgccagg cctcatcacc cttgcgccgg
    10741 aggccgcggc atacccgacc tccccctcgc cgacctcctc ggcgggtgct acggctcacc
    10801 tcccgctgcc ctcggccgcc ccatccggta tgccggtcgc ccccgtcgac acgacggcgc
    10861 ctctgcccag cgtggcgggg atcaaggccg ccgtcgcacc tgcgctggcc gacccggacc
    10921 tcaagggagc cgccgtcgtc gtgcgcgacg cgctcaccgg cgccgacctg ctcgacgtgt
    10981 ccggcagcga gccccggatc ccggcgtcga ccctcaaact cattgccgcc ctggcggtcc
    11041 agcaagtctt ccccgacggg acgacactcg tgacccgcgt cgtccagggc gcgagccctg
    11101 acaccgtggt gctcgtcgcc gggggagaca tgttcctggc gcccgacaag ggcgatccgg
    11161 ccgcgaccgt gggccgggcc gggctgggtg atctggccga ctcggtggct accgcgttgc
    11221 gcgccaaggc gatcacccag gtgaccgtgc aggtggacac ctcctacgcg ccgggtccgc
    11281 aggtggcggc cggctggcaa ccggcgttcc gcgccctcgg gatcgtcggc ccggtggcga
    11341 tgctcggtct gtcgacccaa tgggcccaga gcggcaagcc gggcccggcc gatcccgttg
    11401 ccgaggtggt cgcggccttc gctgcgcggc tcaccgaacg aggcatcacc gccgtggccg
    11461 gggggcgcgc gtccggggcc ggggtatcgc tcggtgaggt ccactccgcg cccgtcaccg
    11521 atgtccttgg cctggccctg gagcgaagcg acaacaccgt gaccgagtcc ctggcccgtc
    11581 aggccgcctt ccgggtgggg cggccgagcg acttcgaggg gacggcggca tacctgcgcg
    11641 aggccctcac cctcgcgggg atcgatccgc gtggcatgag tgtgcacgac gcgtcggggc
    11701 tcagccgcac gagtcacgtc ccggccaggg tcctggccga cgtcctcaac ctcggtgtga
    11761 agggcaccgt cccgggcttc acccaggcgc tgcgccactt gccggtggcg gggctgacgg
    11821 gcaccttggg gacgcgcttc accgcggccc cccaggcccc ggccgccggc ctcgttcggg
    11881 ccaagaccgg ctcgctcaac ggggtgaact cgctcgccgg aacggtcgtc accgcagacg
    11941 gccggatgct cgtctttgcg atcatgcagg aggggccggt gggaacgacc gatgcacggg
    12001 ccgcgttgga ccggttcgga gcggcgctcg cgacctgcgg gtgccgttga ccatgaaggg
    12061 attcccgcgg tgagcgacca gcctgtcgac cgaccgtccg accaccccgg tgacgcgccg
    12121 gggccattca tcgactggga cttcgcgcga gtcaccgcgg cgcgcctggc ttcccccggc
    12181 cccaaggtca acgccggcga ggccgcagga gtggttgccg gcttgcgcca ggcggccctg
    12241 gcggcccatg acccggtcgc tgagacgtcc cgactgacct cgccccccgg gtcggcaccg
    12301 gtcctcatcg tcgaccgagc cgggtgggcg tcagccaaca tcgactcgat gcgcgaactc
    12361 atcgaccccg tcgtggccaa gatggccgcc ggtatgccgt cccagtccgg tgagggaatg
    12421 gccgccatgg gcagcaagat caccggagcc gagactggtg cgctgctggc cttcttctcc
    12481 agcaagatcc tcggccaata cgatctcgcc ccctcgggcc agccgcggct gctcctcgtc
    12541 gcgcccaaca tcgtgtcggc cgagcgtgag atgggcgtct caccccgcga tttccgcctg
    12601 tgggtggcaa tgcacgagga gacccaccgg gtccagttca ccgccgtccc gtggctgcgc
    12661 gagcacatga tcgagcgctc ccgcaccctg gccctcgagt tggcgccgac gccgagtgag
    12721 ttcgccgagc ggatccagca gatcgcccag ggtctgccgg gagccttccg gccaggcagc
    12781 accgggctgg ccgaggtgtt cctcacgcct gagcagaagg gcaagatcgc cgagatcacc
    12841 gccgtgatgt cgctgctcga ggggcacgcc gacgtgatca tggacgacgt cggcccgtcg
    12901 gtcatcccca cggtcgccga gatccgcgag aagttcaccc aacgccgcgc cggcctcggg
    12961 caactcgacc ggctgatccg ccggctcctt gggttggagg ccaagatgcg ccagtatgcc
    13021 gatggcgccg tgttcgtccg ggccgtcgtc gaccgcgtgg gcatcgatgg cttcaacgcc
    13081 gtctggacct ccccgcagac gctcccgacc gccgccgaga tggccgaccc gcaggcgtgg
    13141 gtcgcgcgcg tccacggctg agccgggcgg catacctgtg cctggtcccc cggcagcggt
    13201 ggcggcggtc cgcctggcgg tgcggggggt gttggccgac ctgccgacct ggtcgacggt
    13261 gctggttgcc tgctccggag gtgccgattc tctcgcgctc gcggcagcgc tcgccttcga
    13321 agcccccggg gccggcctgc gcgccggtgc cgtgatcgtc gaccacgggt tgcaggaggg
    13381 ctccgccgac gtcgcggcgc aggcggcgca ggcctgtcgc gggttgggtc tggatccggt
    13441 cgatgtcgtg cgggtcgccg tgcccgactc accgaccggt cccgagggcg ccgcccggac
    13501 ggcccggtat gccgcgctcg cggacgcggc cacacggcat accgcggcct tggtgttgct
    13561 cggccacacc cgcgacgacc aggcggagca ggtgctgctc ggcctcgccc gcgggtccgg
    13621 ggccagatcc ctctccggta tgccgccctc ccgcggtccc tatgcccgtc ccctcttggg
    13681 ggtgacccga gcgcagaccg aggccgcctg ccgcgaggcg ggcctggagc cgtggcggga
    13741 tccgcacaac tccgacccga cctatgcgcg ggtgcgcgcc cgggcggccc tgggggagtt
    13801 gaacgcggtg ctaggccccg ggctcggggc ggcgctggcc cgctcggccg acctgctgcg
    13861 tgatgacgcc gacctgttgg acgagttagc cgctcaggcg cgcgccgacg tggacctcga
    13921 cccggcgggg gaacgggtcg tggctgcgca ggcgggcgcc ggggacgtgc cggccgttcg
    13981 ggtagcggtg gtgggcctgg ccgccctgcc ccccgccttg cgccggaggg tctggcggat
    14041 cgtcgccgcg gaggccgggg cgcgcgcgct gtcggccgcc cacgtcgccc tgctcgacgg
    14101 actggtcctg cggtggcacg ggcaaggggc gatcgacctg ccgggtggcg tggtcgcgct
    14161 acgggtaggt gggaccattc agatgggac
//
LOCUS       DAOMZY010000038        13944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_4005_length_13944_cov_6.516812, whole genome shotgun sequence.
ACCESSION   DAOMZY010000038 DAOMZY010000000
VERSION     DAOMZY010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 13944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13944
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4005_length_13944_cov_6.516812"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(446..1084)
                     /locus_tag="PLK64_03670"
     CDS             complement(446..1084)
                     /locus_tag="PLK64_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPV78868.1"
                     /translation="MNINRLAERARTARDELDEILDAGWVGTLSVSVDGSPQVVPILY
                     ARVGDAVILHGSTGAGSLRAAAAGLPVAFCVAHLDGFVYAASLFESSANYRSAVVYGS
                     CERLTGDAAEAALVGLSEHVMPGRAGEVRANTAKERAATIVLRLPIVDGQWLAKARTG
                     SASVEEGDDPSVWTGVLPVSTVLGLPQPSPETAERGIPVAGSVARRVADQSG"
     gene            1168..2346
                     /locus_tag="PLK64_03675"
     CDS             1168..2346
                     /locus_tag="PLK64_03675"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HPV78869.1"
                     /translation="MPRRSRDAGREVAPIKELDRREVAGAVADLGVLVPIVVALILVN
                     GLSASAVLLPAGLLYVAAGWFYRLPIPVQPLKAFGAIAIAQGLGADVIAAGSLLMALV
                     FIPLGLSGLLDRAAAIFPKPLVRGVQLSVGILLIRLAWGLVVEPPRQFADAGAPTALL
                     VAGALVAGAAAYVWRARGITVVLVGIALVWVVARAHLPMTLGPSELRLHTPSASDFAT
                     AAVVLVVPQLPLTFANSCIATADAARGYFGAAASRVTAGRLAVSLGLGNVLAGLLGGM
                     PVCHGAGGLTAHRSFGARTGRAPIFLGTILVVLALVAGQVTGGALAGFPVWILAALLG
                     VAGVLHIQLLRDLRGRTEWGFAIAAGVLGALVNLAVALAVGLSAWWVAAALRRSRASG
                     "
     gene            complement(2429..3325)
                     /locus_tag="PLK64_03680"
     CDS             complement(2429..3325)
                     /locus_tag="PLK64_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78870.1"
                     /translation="MDADPSVDARLDEAVDELYGLTGDAFLPRRGQLAAAARAGGDTE
                     LAKAITALRKPTQSAWAINLLSRECADDLHRLADLAARLRTAQERLDGAALLTLGRER
                     TALVDELVAQAHVVVTQAGAKFSPAMARDIGTTFVAALASTEATAAVTSGRLTRALEY
                     AGFGEIDLTEATARPLRLVRSDERASLMSSGVRADAHRGASSPDGALADDADPEVSAA
                     EADLHDAMATATAAASRHGVLSAELELAENRVAQLQAELARARAHRDKTADALGEADS
                     AQAAAQRELRRARTALEDLRNG"
     gene            3359..4123
                     /gene="dapB"
                     /locus_tag="PLK64_03685"
     CDS             3359..4123
                     /gene="dapB"
                     /locus_tag="PLK64_03685"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HPV78871.1"
                     /translation="MSQPKVAVIGSRGRMGATVCRAVEAASDLEFVGGFDMGDELGDL
                     GGADVVVEFSVPSASLANVVACVSRGVHVVVGTTGWDDAKLDELRQQLLAAPEARAGG
                     TVGVLIAPNFALGAILMMSFAAQAARFFESVEVVELHHPNKVDAPSGTAARTAELIGA
                     ARDAAGVGPVPDATTTDPDGARGARVHGIPVHSLRVRGLVAHQEVILGNVGETLTIRH
                     DSLDRESFMAGVLAGVRGVAGHPGVTVGLEHYLGLG"
     gene            4261..4971
                     /locus_tag="PLK64_03690"
     CDS             4261..4971
                     /locus_tag="PLK64_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78872.1"
                     /translation="MKRAAIVLASAIAVAAIGGCSSTATTTSTTPAATPATASAAPTA
                     GRMGSGGPPSDRGPGNGVGGGMGNGNGPARATNGAVTAAPPAGTVAAAAWEALMSPEG
                     EYAAAASYQAVIAKFGDVEPYVSIEAMEQNHIQALTRQLSRFGFVPPANPYAGTIAAP
                     ADLVTAAQAWVDGERKNVALYDRLAAAATGDAGVTRVFTNLRRSSLDAHLPLFEAAVK
                     AGGSLTEPQMAELGFVRH"
     gene            complement(5047..5361)
                     /locus_tag="PLK64_03695"
     CDS             complement(5047..5361)
                     /locus_tag="PLK64_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HPV78873.1"
                     /translation="MSLWFTVLAACALAFLTKLAGHYVPSAKLEGRRMRRVTALLPVA
                     LLTSLVVIQTFAASGGGFTVDARIVGLAAAVIALLLRAPFIVVVIIGAAVAAGLRALG
                     WN"
     gene            complement(5358..6122)
                     /locus_tag="PLK64_03700"
     CDS             complement(5358..6122)
                     /locus_tag="PLK64_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HPV78874.1"
                     /translation="MREEAGKEGAEASGQRPGDLDPATRTSVLRQAWSVGIATGAYGI
                     SFGALGVASGLSVAQTCALSLLMFTGGSQFAFVGVIGAGGTGVAAIATATLLGIRNGL
                     YGLQVSEFLHDRGWRRGVAAHLTIDESTAVGIAQPTVRASQLGFWQTGIAVFVFWNLF
                     TLLGAWLGNALGDPRRWGLDAAAAAAFVALLWPRLHSRDAAATAVVAVVLTVAVAPWA
                     PAGIPVLIAAFAAVAVGLWPRAAARDDDSTGGEVAP"
     gene            6169..6645
                     /locus_tag="PLK64_03705"
     CDS             6169..6645
                     /locus_tag="PLK64_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78875.1"
                     /translation="MGDRLPRVRALVGAKADAAATARAVALAVVVAVVVALAGLTACS
                     SAPVGETPESTVAGFYRALGAKDSAAACALVAYDGKPLAGDDVLLCRSGFDTIITDVA
                     TPEELAALTSASVTGAAVDGDQATVTADQMTGVPAAYREDVNLVRVGGRWYIESPL"
     gene            6722..7804
                     /gene="paaA"
                     /locus_tag="PLK64_03710"
     CDS             6722..7804
                     /gene="paaA"
                     /locus_tag="PLK64_03710"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HPV78876.1"
                     /translation="MYGNDFAEPDDPGAGPLNAADGGAVEAAAAHVDTKAREGLASFD
                     AIIAADQRIEPRDAMPEEYRQTLIRQIAQHAHSEIIGMQPEGNWITRAPSLRRKAILI
                     AKVQDEAGHGLYLYSAAETLGITREELLDRLHTGKQKYSSIFNYPTLTWADCGAIGWL
                     VDGAAIMNQVPLCRCSYGPYARAMIRTCKEESFHQRQGFELLWELMRGTPEQQAMAQD
                     ATNRWWWPALAMFGPRDTGEHAVTGGHTEQSMAWGIKRFSNDDLRQKFVDMTVPQAAR
                     LGVTLPDPDLLWNEERGHYDFGEIDWDEFWRVIKGDGACNAARIERRVSSHEQGAWVR
                     EAATAYAAKQRERADGAVGAGEGSAA"
     gene            7801..8088
                     /gene="paaB"
                     /locus_tag="PLK64_03715"
     CDS             7801..8088
                     /gene="paaB"
                     /locus_tag="PLK64_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HPV78877.1"
                     /translation="MSEGTLPLFEVFVRAKRGLSHVHVGSVHASDAAMAVRNARDLYT
                     RRQEGVSIWVVAASDIVASSPDEKDSFFDPAGDKIYRHPTFYEVPEDVEYL"
     gene            8085..>8398
                     /locus_tag="PLK64_03720"
     CDS             8085..>8398
                     /locus_tag="PLK64_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaI"
                     /protein_id="HPV78878.1"
                     /translation="MSEKETGAAYSAYVLGLADDALIYSHRLGEWLTWAPALEEDMAL
                     ANVSLDLIGQARSLLTHVGSLDGTGRSEDDLAYLRDERDFRNVHLVEQRRGDFGQEMA
                     RML"
     assembly_gap    8399..8498
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8499..8691
                     /locus_tag="PLK64_03725"
     CDS             <8499..8691
                     /locus_tag="PLK64_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaI"
                     /protein_id="HPV78879.1"
                     /translation="LPSSLRSPAQASVEAVVAEATLTMPATPKWHSRGGREGVHSEPL
                     GFLLAEMQYLPRSVPGAHW"
     gene            8694..9218
                     /gene="paaJ"
                     /locus_tag="PLK64_03730"
     CDS             8694..9218
                     /gene="paaJ"
                     /locus_tag="PLK64_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HPV78880.1"
                     /translation="MMATATDVLEAVRAIPDPEIPVITIEGLGILRECRLDEAAGLVR
                     VVITPTYSGCPAMRAIEDQIARVCRAHGLRAVVTSQLSPAWTTDWMSPDAREALRRFG
                     IAPPGPADADAGAGERAPRPVSVMIGGGRHTVACPRCGSEATSVISQFGSTACKALRR
                     CDSCGDPFDEFKAL"
     gene            9219..10343
                     /gene="paaK"
                     /locus_tag="PLK64_03735"
     CDS             9219..10343
                     /gene="paaK"
                     /locus_tag="PLK64_03735"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HPV78881.1"
                     /translation="MTGLLSPTAGTTTRRATFHPLTVAAVERLTPEATAITFAVPDDL
                     ADAYRFAPGQHLTLRRTVDGAEVRNSYSICQSAHGGRPGTLRVAAAKVPGGRMSPWLV
                     DELAAGDVVDVMTPLGNFTCPVQPDAARHHVAIAAGSGITPILALVSTALEEEPGSRV
                     TLLFGNRRTSTVMFLEELEDLKDRYGARLHLIHVLSREAPDVELFHGRIDKERLTRIL
                     DALVPVEGIDEVYLCGPFGMVQDAQALLSERGFGKEHVHHEIFHVDEDGAPKPAPVIV
                     DTSAPPAAVVTVALDGRTTVIPMPTREESILDATLRARPDAPYSCTGGVCGTCRARVV
                     SGEVRMDRNYALEPDEVAAGIVLACQAHPVSDTVELDYDA"
     gene            complement(10362..10826)
                     /locus_tag="PLK64_03740"
     CDS             complement(10362..10826)
                     /locus_tag="PLK64_03740"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HPV78882.1"
                     /translation="MTVGMIAAVSRNGVIGDGVRMPWHLPEDLAHFKHTTMGHTLLMG
                     RRTFDSIGRALPGRRSIVLTRNTRWSHADVEVAHTFAEGLALAGPTELWVVGGGEVYR
                     LAMPYAARLVITEVDQDAEGSVTFPAIDARVWVETSRMPHEGFAFVGYERRA"
     gene            complement(10851..11699)
                     /locus_tag="PLK64_03745"
     CDS             complement(10851..11699)
                     /locus_tag="PLK64_03745"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HPV78883.1"
                     /translation="MRQYLDLLDRVLADGVTKSDRTGTGTMSVFGHQMRFDLADGFPV
                     LTTKRLHLRSIIGELLWFLRGDTNVRWLQERGITIWDEWADERGDLGPVYGYQWRSWP
                     TPDGRQVDQIAHVVEAIRRNPDSRRHIVSAWNVADVESMALPPCHALFQFYVRPDVFP
                     AALEAAARGEHTAYPAFLDCQLYQRSADIFLGVPFNIASYALLTAMVAQVTGLRPGHF
                     VHTLGDAHLYLNHLDQARLQLTREPRPLPTLRLTPGLGLDEFDLADVELVGYDPHPGI
                     KAPIAV"
     gene            11943..12830
                     /gene="dapA"
                     /locus_tag="PLK64_03750"
     CDS             11943..12830
                     /gene="dapA"
                     /locus_tag="PLK64_03750"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPV78884.1"
                     /translation="MTSPIFGRVLTAMVTPMTPAGEVDEDAVLAVADHLLAHSHDGIV
                     VNGTTGESATLTSDESIRVIELVAAHVGDRAVVVAGVGSNDTAHSIDMARRAAAVGAR
                     GLLVVTPYYNKPTQPGLVAHFGAVADATDLPVLVYDIPGRTGIPIQTETLIRLAEHPR
                     IVAVKDAKGDLWAASQVMAATDLQWYSGDDVLNLAHLAQGAVGVVSVVGHVAGDEYAA
                     MVRAVADGDLATARSIHRRLIPVVQAIMSPSQGAIMAKAALAELGVIPSAFVRLPLVE
                     SPPEHLAMLREGLSQSGIL"
     gene            12827..>13944
                     /locus_tag="PLK64_03755"
     CDS             12827..>13944
                     /locus_tag="PLK64_03755"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HPV78885.1"
                     /translation="MSHPHPELTLPPPLAPGALRIIALGGLGEIGRNMTVLETGGRLL
                     VIDCGVLFPEDHHPGVDLILPDFSYLDGRLDDIEAVVLTHGHEDHIGAVPFLLARKRD
                     IPLIGSQLTLALVEAKLKEHRIKPYTLTVKEGQREKLGAFDLEFVAVNHSIPDALAVM
                     VRTAAGSLLHTGDFKMDQLPLDGRITDLRAFARLGEEGVDLFLTDSTNADVPGFTAPE
                     RDISSAIDKVFRHAQRRIIVACFSSHVHRVQQVLDAADKAGRKVALVGRSMVRNMTIA
                     ADLGYLHVPPGVLIDLKRVDDLPDDQIVLVSTGSQGEPMAALARMANRDHRIVVGPGD
                     TVLLASSLIPGNENAVYRVINGLMRLGAHVVHKGNAKV"
BASE COUNT         2073 a         4931 c         4808 g         2032 t
ORIGIN      
        1 gcgatccacc tgacaccttc acgttggtcc cgtccccatc cgcggccgat ccctgggcag
       61 atctgtgggc tgactcgcga gacccggacg aacaacccac gacggatcac tctgcccgag
      121 accgggacca catcaacccc gaagcagccc cctggggaga cctgtgggac gacactcctc
      181 ggcgagggtc ggagtcgaca gatcacccgc cacggcacga cgccaacccc gaagcagacc
      241 cctggtaccg ggccgaacac ccgaacgatc tgcggccgcg ggacgaccct gcccgggatc
      301 gagacgccgc caatcccgaa gcggatccct gggctgacct ctgggcagac tcccgaaacc
      361 gtgacgaccg cccggcaaca ggccacgagg accgccacga tgccaacccc gaggcgaacc
      421 cctggttcga cgcgggccct gccgcctaac ccgactggtc cgcgacccga cgggccaccg
      481 accccgccac cggtatgccg cgctcggccg tctcggggga gggctgcgga aggccgagga
      541 ccgtgctcac cgggaggacc ccggtccaca ccgacgggtc gtcgccctcc tccacacttg
      601 ccgacccggt gcgtgccttg gccagccact ggccgtcgac gatgggcaga cgcaggacga
      661 tcgtcgccgc gcgttccttg gcggtgttgg cccggacctc accagcgcgc ccaggcatga
      721 cgtgctccga gagccccacc aaggcagcct cggcggcgtc gccggtcagg cgctcacagc
      781 tgccatagac gaccgccgag cggtagttgg cgctggactc gaacagcgac gcggcataca
      841 cgaagccgtc gagatgagcc acgcagaagg cgaccggcaa cccggccgcg gcggcgcgca
      901 ggctgccggc tccggtggag ccgtggagga tgaccgcgtc gccgacgcgc gcatagagga
      961 tcgggacgac ctgcgggctc ccgtcgacgc tcaccgacag ggtgcccacc caaccggcgt
     1021 caaggatctc gtcgagctcg tcgcgagcgg tgcgggcgcg ttcggcgagg cggttgatgt
     1081 tcatgggtcg atcctctcgg tttgcgccac ccggtcgcac agacatttca cgtcggggag
     1141 gctgcgtcgg catgggacca tcggcgggtg ccccgccgat cccgagacgc cggccgcgag
     1201 gtggcgccca tcaaggagct ggaccgccgc gaggtcgccg gtgccgtcgc tgacctcggc
     1261 gtgctcgtcc cgatcgtcgt ggcgctcatt cttgttaacg ggctctcggc cagcgcggtg
     1321 ctgttgccgg ccggcctgct ctatgtggcc gcgggctggt tctaccgtct gccgattcct
     1381 gtgcagccgc tcaaggcctt cggagcgatc gccatcgccc aggggctcgg cgcggacgtc
     1441 atcgcggccg ggtcgctgct catggcgctg gtattcatcc cgctcgggct tagcggtctg
     1501 ctcgatcgtg ccgcggcaat ctttcccaag ccgctcgtcc gtggagtgca gctgtcggtc
     1561 ggcatcctgc tcatccgcct tgcctggggt ctggtggtcg agccgccccg ccagttcgcc
     1621 gacgcaggag cgccgaccgc gctcctcgtg gccggtgctc tcgtcgccgg ggccgcggca
     1681 tacgtctggc gggcccgggg gatcaccgtc gtcctcgtcg gaatcgcgct ggtgtgggtg
     1741 gttgcccgag cccacctccc gatgaccctc ggcccttcgg aactgcgcct gcacacgccg
     1801 tctgcaagcg attttgctac ggccgcagtg gtgttggtcg tgccgcagtt gccgctcacc
     1861 ttcgccaact cctgcatcgc caccgccgac gcagctcgcg gctacttcgg tgcagccgca
     1921 tcgcgggtca ccgccggacg gctggccgtg tcgctcgggc tgggcaacgt cctcgccgga
     1981 ctcctcggag gtatgccggt ctgtcacggc gcggggggcc tcaccgctca ccggtccttc
     2041 ggcgctcgga ccggccgggc accgatcttc ctcggcacca tcctcgtcgt gctggccttg
     2101 gtggccggtc aagtcaccgg gggtgcgctc gcgggtttcc ccgtgtggat cctcgccgcg
     2161 ctgctcggag tcgccggggt gctgcacatc caacttctgc gcgatctgcg gggtcgcacc
     2221 gagtggggct tcgccatcgc ggccggcgtg ctgggagcgt tggtgaacct cgcggtggcg
     2281 ctggcagtgg ggctgagcgc ctggtgggtg gctgccgcct tgcgccggag ccgggccagc
     2341 ggctgaccac tgcaggatga cgggggccgt gagcaggcgg tcggccgggg cttggccagt
     2401 gcccggggag tgcacagccg gttgacactc agccgttgcg caggtcctca agtgccgtgc
     2461 gggcccggcg caactcgcgt tgggctgcag cctgcgcgct gtcagcctcg ccgagcgcgt
     2521 ccgcggtctt gtcccggtgc gctcgggccc gggcgagctc ggcctggagt tgagcaacgc
     2581 ggttctcggc gagctcgagc tcggcgctga ggactccgtg acgggatgcc gccgctgtgg
     2641 cggtggccat ggcgtcgtgc aggtcggcct cggccgccga cacctcggga tcggcgtcgt
     2701 ccgcgagtgc cccatccggt gacgaggcgc cccggtgggc gtcggcacgg acgcccgacg
     2761 acatcagcga ggcgcgctcg tcggatcgca cgagacgaag cgggcgagcg gtggcttcgg
     2821 tgaggtcgat ctcgccgaaa ccggcatact ccagggctcg ggtgagccga ccggaggtga
     2881 cggccgccgt ggcctcggtg gaggcaaggg ccgcgacaaa ggtggtgccg atatcgcgcg
     2941 ccatcgccgg gctgaacttg gcccccgcct gggtgacgac cacgtgggcc tgcgccacca
     3001 actcgtcgac gagcgcggtg cgttcgcggc cgagggtgag cagcgcggcg ccgtcgaggc
     3061 gctcctgagc cgtgcgcagc cgcgctgcga gatccgccag ccgatgcaga tcgtcggcac
     3121 actcgcggga gagcaggttg atcgcccagg ccgactgggt gggcttgcgc agcgccgtga
     3181 tcgccttggc gagctccgtg tcaccaccag cccgggcggc cgccgccaac tgacctcggc
     3241 gcggcaggaa cgcgtcgccg gtcaacccgt agagctcgtc gaccgcctcg tcgaggcggg
     3301 cgtccacgct cgggtcggcg tccatgggcg gaatcgtccc acggctaccc ttgcgctcat
     3361 gagccagccg aaggtcgccg tcatcggatc ccgcggtcgg atgggagcga cggtatgccg
     3421 cgccgtcgag gccgcgtccg atctggagtt cgtcggtggt ttcgacatgg gcgacgagct
     3481 gggcgatctc ggcggagctg acgtcgtcgt cgagttctcg gtgccctcag cgtcgctggc
     3541 caatgtcgtc gcctgcgtca gccggggtgt gcacgtcgtt gtggggacga ccgggtggga
     3601 cgacgccaaa ctcgacgagt tgcgccaaca attgctcgcg gcgcccgagg cgcgcgccgg
     3661 cggaacggtc ggggtgctca tcgcccccaa cttcgccctc ggcgccatcc tcatgatgtc
     3721 gttcgccgct caagcggcga ggttcttcga gtcggtcgag gtcgtcgaac tgcaccaccc
     3781 caacaaggtc gatgcaccct cgggcaccgc cgcgcgcacc gccgagctca tcggggccgc
     3841 gcgggacgca gccggagtcg ggccggttcc cgacgccacc acgaccgatc cggacggtgc
     3901 ccgcggcgcg cgcgtgcacg gcataccggt gcactcgctg cgggtgcgcg ggctggtggc
     3961 gcaccaggag gtcatcctcg gcaacgtggg ggagacgctg acgatccggc acgactcgct
     4021 ggaccgcgag tcgttcatgg cgggcgtgct cgccggcgtg cgaggcgtcg ccgggcatcc
     4081 gggcgtcacg gtcggcctgg agcactacct cggcctcggc tgacgggggc ggcccgcggc
     4141 gccgctgcgg tcgctcgccc ctcggcgaga tctctcgcaa cgcccacgcg cgcttcacag
     4201 tggcttcatg aatgcctcgg agactgaggt cgatcccagc tccgagcaaa ggaacccgtc
     4261 atgaagcgcg cagccatcgt cctcgcctca gccatcgccg tggccgcgat cggtgggtgt
     4321 tccagcaccg cgacgaccac cagcaccact cccgccgcaa cgccggcgac tgcctcggcg
     4381 gccccgaccg ctgggcggat gggctccggc ggcccgccgt ccgaccgtgg ccccggaaac
     4441 ggtgtcggag gcgggatggg caacggaaac ggccctgccc gcgccacgaa cggcgcggtc
     4501 accgccgccc cacccgccgg caccgtcgcc gcggcagcct gggaggcgct catgagcccg
     4561 gaaggggagt atgccgccgc agcctcctat caggcggtca tcgcgaagtt cggagacgtc
     4621 gagccctacg tcagcatcga agcgatggag cagaaccaca tccaggcgct gacccggcaa
     4681 ctgagtcggt tcggcttcgt cccgccggcc aacccctatg ccggcacgat cgcggcccca
     4741 gccgacctgg tcaccgcggc ccaggcgtgg gtcgacgggg agcgcaagaa cgtcgcgctc
     4801 tatgaccggc tcgcggcggc cgcgaccggc gatgccgggg tgaccagggt cttcaccaac
     4861 ctgcgccgtt cctcgctcga cgcccacttg cccctgttcg aggcggcagt gaaggccggc
     4921 ggctccctca ccgagccgca gatggccgag ttggggttcg tgcggcactg acgtcgagtg
     4981 gtgtgatgag caggacgttg ggctggaggt ggccggttgg accggtcagt tccagcccaa
     5041 cgcgcgttag ttccagccca acgcgcgaag tccggcggcg acggcagcgc cgatgatgac
     5101 gacgacgatg aacggcgcgc gcagcaacag cgcgatcacg gcggcggcca ggccgacgat
     5161 tcgggcatcg acggtgaagc cgccaccgga ggcggcgaag gtctggatga cgaccaacga
     5221 cgtgagcagc gccacgggca gcagggccgt gacccggcgc atccgccggc cctcgagctt
     5281 ggccgacggc acgtagtgcc cggcgagttt ggtgaggaac gcgagcgcgc aggccgcgag
     5341 gacggtgaac cagaggctca cggcgcgacc tccccaccgg tgctgtcgtc gtcgcgggca
     5401 gcagcgcgcg gccacagccc gacggccacg gccgcgaacg cggcgatgag cacgggtatg
     5461 ccggccggcg cccacggcgc gacggccaca gtgagcacga ccgccaccac ggcggtggcg
     5521 gccgcatccc gagagtgcag cctcggccag agcagggcga cgaaggcagc ggcggcggct
     5581 gcgtccaggc cccatcggcg tgggtcgccc agggcattgc cgagccacgc cccgaggagg
     5641 gtgaacaggt tccagaagac gaagacggcg atccccgtct gccagaagcc cagctgggac
     5701 gcccgaaccg tgggctgagc gatcccgacg gccgtcgact cgtcgatcgt caggtgggcg
     5761 gcgaccccac gtcgccagcc gcggtcgtgg aggaactccg acacctgcag gccgtagagc
     5821 ccgttacgga tcccgagcag ggtcgccgtg gcgatggccg cgacccccgt gccaccggcg
     5881 ccgatgacgc cgacgaaggc gaactgcgac cctcccgtga acatcagcag ggacagggcg
     5941 caggtctgcg ccacgctcag ccccgacgcc acgcccaagg ctccgaacga gatcccgtag
     6001 gcccccgtcg cgatccccac cgaccaggcc tggcgcagaa ccgacgtgcg cgtcgcggga
     6061 tcgaggtcgc ctggccgctg cccgctcgcc tccgcgccct ccttgcccgc ctcctcgcgc
     6121 attcgccgat ggtgacagag ggccgtgtgc gcatgtttgg atggtccggt gggtgatcgt
     6181 ctaccgcgtg ttcgggccct tgtgggcgcc aaagccgacg ccgctgccac cgccagagcc
     6241 gtcgccttgg cggtcgtcgt ggcggtcgtc gtggcgctgg ccgggttgac ggcgtgctcg
     6301 tcagcacccg tcggcgagac gccggaatcg acggtcgcgg ggttctatcg cgcgctgggc
     6361 gccaaggact cggccgccgc gtgcgcgttg gtcgcctacg acggcaagcc cctggccggc
     6421 gatgacgtcc tgctgtgccg atcgggcttc gacacgatca tcacggacgt ggcaacaccc
     6481 gaggagttgg ctgctctcac ctcggcgtcc gtcacgggtg ccgcggtcga cggcgaccag
     6541 gcaacggtca cagccgacca gatgaccggc gtgcccgcgg cataccggga ggacgtgaac
     6601 ctcgtgcgcg tgggcggacg ctggtacatc gaaagcccgc tctgaccttg gtctgacggc
     6661 tgttgtgatg ttcacccctc cagagggggc gaggcgatcc cggcccggcc atactgtgct
     6721 catgtacggc aacgacttcg ccgagcccga cgacccgggg gcaggccccc tcaacgcggc
     6781 ggacggagga gcggtcgagg ccgcggcggc ccacgttgac accaaggccc gtgaaggcct
     6841 ggcgagcttc gatgcgatca tcgccgccga tcaacggatc gagccgcgcg acgcgatgcc
     6901 cgaggaatat cgccagaccc tcatccgcca gatcgcccag cacgcacact ccgagatcat
     6961 cgggatgcag ccggagggca actggatcac ccgggcaccc tcgctgcggc gcaaggcgat
     7021 cctcatcgcc aaggtccagg acgaggccgg ccacgggctc tacctctact ccgcggcgga
     7081 gaccctcggc atcactcggg aggaattgct cgaccggctg cacaccggca agcagaagta
     7141 ctcctcgatc ttcaactatc cgacgctcac ctgggccgac tgtggcgcca tcggctggct
     7201 cgtcgacggc gcggcgatca tgaatcaggt gccgctgtgt cggtgctcct atggtcccta
     7261 cgcccgggcg atgatccgca cctgcaagga ggagtccttc caccagcggc agggcttcga
     7321 gttgctctgg gagctcatgc ggggcacccc cgagcagcag gcgatggctc aggacgcgac
     7381 gaaccggtgg tggtggccgg cgctcgcgat gttcgggccg cgcgacaccg gtgagcacgc
     7441 ggtcaccggc ggccacacgg agcagtcgat ggcgtggggc atcaagcggt tcagcaacga
     7501 cgacctgcgt cagaagttcg tcgacatgac cgtcccgcag gccgcccggc tcggcgtgac
     7561 gctgcccgac cctgacctgt tatggaacga ggagcgcggg cactacgact tcggcgagat
     7621 cgactgggac gagttctggc gggtcatcaa gggggacggc gcctgcaacg ccgcccggat
     7681 cgagcgccgg gtctcctcgc acgagcaagg cgcgtgggtg cgcgaggcag cgacggcgta
     7741 tgccgccaaa caacgtgagc gtgccgacgg cgccgtcggc gcgggtgaag ggagcgcggc
     7801 atgagcgagg gaaccctccc gctgttcgag gtcttcgtgc gggccaagcg gggcctgtca
     7861 cacgtccacg tggggtcggt gcatgcctcc gatgccgcga tggccgtgcg caacgcccgt
     7921 gacctctaca cccgccgcca agagggtgtc tcgatctggg tcgtggcggc cagcgacatc
     7981 gtcgccagca gccccgacga gaaggactcc ttcttcgacc cggcgggcga caagatctac
     8041 cgccacccga cgttctacga agttcccgag gatgtcgaat acctgtgagc gagaaggaaa
     8101 ccggcgcggc atacagcgcc tatgtcctgg gcctcgctga cgatgccctc atctactccc
     8161 accggctggg ggagtggctc acctgggcgc cggcgctcga ggaggacatg gccctggcca
     8221 acgtctccct cgacctcatc ggtcaggcgc gctcgctgct gacccacgtc gggagcctgg
     8281 acggcaccgg gcgctccgag gacgacctgg cctacctgcg cgacgaacgc gacttccgca
     8341 acgtccacct cgtggaacag cgccgcggcg acttcggcca ggagatggcc cggatgctnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc tgccctcgtc gctgcgcagc
     8521 ccggcacagg cgtccgtcga ggcggtcgtc gccgaagcca cgttgaccat gccggcaacg
     8581 cccaagtggc actcgcgcgg cgggcgtgaa ggtgtgcact ccgagccgtt gggcttcctg
     8641 ctcgccgaga tgcaatatct cccgcgctcg gtgcccggag cccactggtg acgatgatgg
     8701 ccacggccac cgacgtcctg gaagcggtgc gggcgatccc cgacccggag atccccgtca
     8761 tcaccatcga ggggctgggc atcctgcggg agtgtcggct cgacgaggcg gccgggctgg
     8821 tccgggtggt catcaccccc acctattcgg gttgtcccgc gatgcgcgcc atcgaggacc
     8881 agatcgcccg ggtatgccgt gcccacggtc tgcgggccgt cgtcacctcc cagctgtccc
     8941 cggcctggac gaccgactgg atgtcgccgg atgcccgtga ggcgctgcgt cgcttcggga
     9001 tcgccccacc cgggccggcc gatgccgacg ccggtgccgg tgagcgggcg ccccgcccgg
     9061 tgtcggtgat gatcgggggc ggacggcata ccgtcgcgtg cccgcgctgc ggcagcgagg
     9121 cgacctcggt gatctcccaa ttcggctcca ccgcctgtaa agccctgcgc cgttgcgaca
     9181 gttgcggcga tcccttcgac gagttcaagg cgctctagat gaccggactg ctcagcccca
     9241 ccgccggcac gacgacgcgg cgagccacct tccacccctt gaccgtggcc gcggtcgagc
     9301 ggctcacgcc ggaggcgacg gcgatcacgt tcgccgtccc cgacgacctc gccgacgcct
     9361 atcgcttcgc cccgggccag cacctcacgc tgcgccgcac ggtcgatggc gccgaggtcc
     9421 gcaactccta ctcgatctgc cagtcggccc acggtggccg cccggggacc ctgcgggtgg
     9481 cggcggccaa ggtgcccggc ggtcgcatgt cgccgtggct ggtcgacgaa cttgccgcgg
     9541 gcgacgtcgt cgatgtcatg accccgctgg ggaacttcac ctgcccggtc caacccgacg
     9601 cagcccgcca ccacgtcgcg atcgccgctg ggtcggggat cactccgatc ctcgcgctcg
     9661 tctccacggc gctggaggag gagcccggct cccgggtcac cctgctgttc ggcaacaggc
     9721 ggacctcgac ggtgatgttc cttgaggagc tcgaggacct caaggaccgt tacggcgccc
     9781 ggctgcacct catccacgtg ctttcacgcg aggctcctga cgtcgagttg ttccacgggc
     9841 ggatcgacaa ggagcgactc acccggatcc tcgacgcgct ggtgccggtc gaaggcatcg
     9901 acgaggtcta tctctgcggg ccgttcggga tggtccagga cgcgcaggcg ctgctgtccg
     9961 agcgtggttt cggcaaggag cacgtccacc acgagatctt ccacgtcgac gaggacgggg
    10021 cgcccaagcc ggcgccggtc atcgtcgaca cctcggcccc gccggcggcg gtcgtcaccg
    10081 tggcgctcga cggacgcacg acggtcatcc ccatgccgac ccgcgaggag agcatcctcg
    10141 atgcgacgct acgggcccgg cccgacgcgc cctattcgtg caccggcggc gtctgcggca
    10201 cctgtcgcgc gcgcgtggtg tccggtgagg tgcggatgga ccgcaactac gcccttgagc
    10261 ccgacgaggt cgccgcgggg atcgtcctgg cctgccaggc gcacccggta agcgacacag
    10321 tcgagctcga ctacgacgcc tgaggagtca ggcgtcaggc gtcaggcgcg gcgctcgtaa
    10381 ccgacgaacg cgaagccctc gtgcggcata cgtgaggtct cgacccagac ccgcgcgtcg
    10441 atcgcgggga aggtcaccga gccctcggcg tcctggtcga cttcggtgat gaccagacgg
    10501 gcggcatacg gcatcgcgag ccgatagacc tcgccgccgc cgacgaccca caactcagtg
    10561 gggccggcga gggcgagccc ctcggcgaac gtgtgggcga cctcgacgtc ggcgtgcgac
    10621 cagcgggtgt tgcgggtgag gacgatgctg cgccgccccg gcagcgctcg gccgatcgag
    10681 tcgaaggtcc gccgccccat gagcaacgta tggcccatcg tcgtgtgctt gaagtgagcg
    10741 aggtcctcag gcaggtgcca gggcatccgg acgccgtcgc cgatgacacc gttgcgagac
    10801 accgccgcga tcatgccaac cgtcatagga ccagtctcct ctggcgccgg tcagaccgcg
    10861 atgggggcct tgatgcctgg gtgcgggtca tatcccacga gctcgacgtc agcgaggtcg
    10921 aactcgtcca ggccgaggcc cggggtcagc cgcagagtcg gcaggggccg cggctcgcgg
    10981 gtcagctgca ggcgggcctg gtcgaggtgg ttgaggtaga ggtgggcgtc accgagggtg
    11041 tggacgaagt gccccggccg cagcccggtc acctgggcga ccatggccgt gagcagtgcg
    11101 taggaggcga tgttgaacgg cacgcccagg aagatgtcgg ccgagcgctg atagagctgg
    11161 caatccaaga acgcggggta tgccgtgtgc tcacctcgcg cggctgcctc gagtgccgcg
    11221 gggaagacgt cggggcggac gtagaactgg aagagggcgt ggcacggggg cagcgccatc
    11281 gactcgacgt cggcgacgtt ccaggccgag acgatgtgtc gtcgggaatc ggggttgcgc
    11341 cggattgcct cgacgacgtg ggcgatctgg tcgacctggc ggccgtcggg ggtgggccag
    11401 gagcgccact ggtagccata gaccggccca aggtcgccgc gctcgtcggc ccactcgtcc
    11461 cagatggtga tgccgcgctc ttggagccag cgcacgttgg tgtcgccgcg caagaaccac
    11521 agcaactcgc caatgatgga gcgcaggtgc agccgcttgg tggtcagcac cgggaagccg
    11581 tcggccaggt cgaagcgcat ctggtggccg aacacactca tcgtcccggt gccggtccgg
    11641 tcggacttgg tcacaccgtc ggcgaggaca cggtcgagga ggtcgaggta ctggcgcacg
    11701 ttcggcaccg taacacccgg cggggacagc ccagacagtg tgacctagat cacacttaac
    11761 tgaggtgtag cgccgtaacg aaccacccgg ccccagcgat gtaggtgttg tcgaggcgct
    11821 acccggaccc ccgagcgggt agcgcctcga cctttctgtt tcttgcccca gatcccgcag
    11881 cggccgactg cagcgcgtgt gcagtgtccg gccccggaac tcgtgcgcga tagcctgagc
    11941 ccatgacgag ccccatcttc ggccgcgtgc tcacggcgat ggtcaccccg atgacgcccg
    12001 ccggagaggt cgacgaggat gccgtcctcg ccgtcgccga ccacctgctg gcccactccc
    12061 acgatggcat cgtcgtcaac ggcacgacgg gggagtccgc gaccctcacc tccgacgaga
    12121 gcatccgggt catcgagctc gttgccgccc acgtgggcga tcgggcggtc gtcgtcgcgg
    12181 gggtcggctc caacgacacg gcgcacagca tcgacatggc ccgccgggcc gccgccgtgg
    12241 gcgcccgagg cctgctcgtc gtcacgcctt actacaacaa gccgacccag cccggtctcg
    12301 tggcgcactt cggggccgtc gcggacgcca ccgacctccc ggtcctcgtc tacgacatcc
    12361 ccggtcgcac cggcataccc atccagaccg agaccctcat ccgcctggcc gagcaccctc
    12421 gcatcgtcgc ggtcaaggac gccaagggcg acctttgggc cgcgagccag gtgatggcgg
    12481 ccaccgacct gcagtggtac tccggtgacg acgtgctcaa cctcgcgcac ctcgcgcagg
    12541 gcgcggtcgg tgtcgtctcc gtcgtgggtc acgtcgccgg cgatgagtat gccgcgatgg
    12601 tccgcgcggt cgcggacggc gacctggcga cggcccggtc gatccaccgc cgcctcatcc
    12661 ccgtcgtgca ggcgatcatg tcgcccagcc agggcgccat catggccaag gccgccctcg
    12721 ccgaactcgg cgtcatcccc agcgcgttcg tccggctccc gctcgtggag tcgccacccg
    12781 agcacctcgc gatgttgcgc gagggcctct cccagtcagg aatcctttga gccatcccca
    12841 tcccgagctg accctcccgc cgccgctcgc tccgggagcg ctgcggatca tcgccctcgg
    12901 tgggctcggg gagatcggtc gcaacatgac cgtcctcgag accggcggtc gcctgctcgt
    12961 catcgactgc ggtgtcctct tccccgagga ccaccacccc ggcgtcgacc tcatcctccc
    13021 ggacttctcc tacctcgacg ggcgcctgga cgacatcgag gcggtcgtgc tcacccacgg
    13081 ccacgaggac cacatcgggg ccgtcccgtt cctgttggct cgcaagcggg acatcccgct
    13141 catcggttcg cagctcaccc tcgccctcgt cgaggccaag ctcaaggaac accggatcaa
    13201 gccgtacacg ctgaccgtca aggagggcca gcgcgagaag ctcggcgcct tcgatctcga
    13261 gttcgtcgcg gtcaaccact cgatccccga tgcgctggcc gtcatggtgc gcacagcggc
    13321 cggttcgctg ctgcacaccg gcgacttcaa gatggaccag ttgcccctcg acggccggat
    13381 caccgacctg cgggccttcg cccgcctcgg tgaagagggc gtggacctct tcctcactga
    13441 ctcgaccaac gccgacgtgc cgggcttcac cgcccccgag cgcgacatct cctcggccat
    13501 cgacaaggtc ttccgccacg cacagcggcg gatcatcgtc gcctgcttct cctcccacgt
    13561 gcaccgggtg cagcaggtcc tcgatgccgc cgacaaggcg gggcgcaagg tggctctcgt
    13621 cggccgctcg atggtgcgca acatgaccat cgccgccgac ttgggctatc tccacgtgcc
    13681 tccgggggtc ctcatcgacc tcaagcgggt cgacgacctg cccgacgacc agatcgtgct
    13741 ggtgagcacc ggcagccagg gcgagccgat ggcggcgctg gcgaggatgg ccaaccgcga
    13801 ccaccggatc gtcgtcgggc cgggcgacac cgtgctgctg gccagttcac tcatccccgg
    13861 caacgagaac gccgtctatc gggtgatcaa cgggctcatg cgcttgggtg cccacgtcgt
    13921 gcacaagggc aacgccaagg tcca
//
LOCUS       DAOMZY010000039        13943 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_4008_length_13943_cov_6.609303, whole genome shotgun sequence.
ACCESSION   DAOMZY010000039 DAOMZY010000000
VERSION     DAOMZY010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 13943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13943
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4008_length_13943_cov_6.609303"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PLK64_03760"
     CDS             <1..277
                     /locus_tag="PLK64_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78886.1"
                     /translation="VEVELIVAAPHFQFTISHSGATSLSVAALRERLDDIACQLSVGE
                     RRPEKGGGVQVKAGTIARLNRESDYGLRPHPSRWEPAPATVSEEGSR"
     gene            274..963
                     /locus_tag="PLK64_03765"
     CDS             274..963
                     /locus_tag="PLK64_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPV78887.1"
                     /translation="MTTPLTRAAAITAAPPGTRNQVISVVLIDDHDLVRQGIASLLSL
                     DRSLHVVAQGASLAEGMVLARSIVADVAVIDVSLGDGSGLDLVAEFRRRHPAMGLVVL
                     TMHQDDDTLLHALDAGASALIHKSRSAEDVIAIVHQAAASPERFSAVGLADAIRRRSS
                     VATVRLTTRESEVLRELAAGASVAQIARALYMSESTVKTHIAKLYDKLGANNRASAVM
                     SAMRLGLIPAP"
     gene            complement(1060..1977)
                     /locus_tag="PLK64_03770"
     CDS             complement(1060..1977)
                     /locus_tag="PLK64_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPV78888.1"
                     /translation="MAWLVFAITLVCLALGVLVYVAATSRSEPTGVARSLAIVERRID
                     RHEVARNELGARDRLLVPILHRTRNLGERLSPSGATQRLARLLDEAGNPSGWTTESLM
                     GAKGAGLIGGLLLGLLIGGFSLRGLIWCLVLAAGIFFLPDLLVYNMGLRRQEEMRRGL
                     ADALDMLTVCVEAGQGFNAALMQVARSVRGPVAGEFARVLSEIQIGRSRADAFAAMGE
                     RTKVPEIRTFVSALVQADRLGLPIANVLREQAKEMRVARRQHAEERAQKVPVKIIFPV
                     LLCIFPSIFVVILGPAVIRIIGVFFSGDL"
     gene            complement(1979..3685)
                     /locus_tag="PLK64_03775"
     CDS             complement(1979..3685)
                     /locus_tag="PLK64_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPV78889.1"
                     /translation="MRRAVFLVVDTSGSMKANSGMPIIRAAVAEFLNRVPADVSVGVI
                     SFANVAGVDVDLTRDKAAIRTATDTLIAGGETALFDGVTLALEKLGATGERSIVVLSD
                     GVNTAGRKDLAAVLNAVTQGGVRVDTIGLRTKVTADEVLIKLAAAGGGNFAPADDQAA
                     VTGAFNASARALDRQVMWSVRPGVLPLGSTPVEVRGMASGTPFSATGTVTVSAVPTAL
                     PTLTLAPALPEASAAVAAPVETVAKWFLVLGIAGTFLGLLGLTAAAVMPTMRTKRENR
                     IQNLEAYSARGLQITKPRRDTQSVITANLVDIGERVMAGRESTSQTMALLQRADLAWR
                     PGEWAVLRLVGVIVGVIAGALVFRDGLVAVFGIVVGALAGLVLPSVSLRLLARRRAAK
                     LERQLPDILLLLASSLSTGFSLPQAMDAVAKDVSEPAAKEFARVIAETRIGSDLESAL
                     ERMAVRMDSDNMRWTSMAIGIQRQVGGNLAETLRTTAATLREREYLRRHVRALSAEGR
                     LSAYILIALPIALFVYEVNVNRSYISLLWTTVLGWIMLVGAVILMAIGVFWMSKAVKV
                     EV"
     gene            complement(3964..5346)
                     /locus_tag="PLK64_03780"
     CDS             complement(3964..5346)
                     /locus_tag="PLK64_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011775561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HPV78890.1"
                     /translation="MSLYDRLETVRRTQTPTTGARLGDAPARPHAPARASLDPFAAVK
                     AAVHQTLLDTLGPQLYDPNLGQVDLDQRVRQTLQVVIDGQQTPLSAADRTRIAQEVSD
                     EILGHGPLEPYLRDNDVTEVMVNGHENIYVERSGRIYRVDAKFTSEAHLRRTIDKIVG
                     RIGRRVDEASPMVDSRLPDGSRVNAVIPPVALDGSMLTIRKFAADPFTARDLIAFGTI
                     SESVAEFLSACVRGRRNIIISGGTGSGKTTTLNVLSSYVSNDERIITIEDAAELQLHQ
                     QHVLRLESRPSNIEGRGQIAIRDLVRNSLRMRPDRIIVGEVRDGAALDMLQAMNTGHD
                     GSITTVHANSPRDSLSRLETMVLMAGVELPLRAIRDQVASAVDLVIQQARLRDGSRRV
                     VSIVEVEGMEGDIITLQDIFSFDYKAGRDENGRYLGGLVSTGLRPKFLDVLADQGIVL
                     PQTMFVRGNR"
     gene            complement(5343..6512)
                     /locus_tag="PLK64_03785"
     CDS             complement(5343..6512)
                     /locus_tag="PLK64_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPV78891.1"
                     /translation="MTILWDRDPASAERFSFALGPNATRVDSGAALSRALSASPDEDA
                     VIVGPDVDLDSACGVVAGLRVEHTALGVILVRRRLDVNILAQALRAGFREVVSADDLP
                     AVADALRRSRELSFRLRGVSADSASRAGKIVTVFSAKGGVGKTTFSTNLGAYLASVGH
                     RTLVVDLDLEFGDVGIALQMLPERSMTDVVNMTGHLDEQGLASVVTIHSSGLEAVCAP
                     AEPGEAERIPPATVAELLRVARYVYDFVVIDTPPALTEHVLTAFDLSDSLVLIATLDI
                     PALKNLRLTLDTLDLLGSPREARHIVLNRSDAKVGLRVEDVVTSIKQEIAVMVPSSPD
                     VPASINRGVPIILDDPRHPVSVALRHLADQHIRGVDPAASQSAAPRRLLPWGGRK"
     gene            complement(6509..7288)
                     /locus_tag="PLK64_03790"
     CDS             complement(6509..7288)
                     /locus_tag="PLK64_03790"
                     /inference="COORDINATES: protein motif:HMM:NF028286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcpC/CpaB family pilus assembly protein"
                     /protein_id="HPV78892.1"
                     /translation="MGRRAIAVLLAGLIAVLGAAAVLIYARGADARAVAGADPRTVYV
                     SEKPVSAGTSLADAVRDRALVKTQVAARAFPVGALAEVTPTNEALVAITDIGVGEYVL
                     ASRFGTTPTGRRAIQVPPGQVAVSVRLEDPARVGSFVTPGSRIVIFDTFEMPAPTSSP
                     TPTAGNPTQTQATRVLLGDALVIAMGDTSLTPADTNTQNGAQDQTKPTFLVTLALTPL
                     DANRLVHGIQTGALYAGLRGADTTIDPTVQITDYNVFEVAP"
     gene            complement(7347..8576)
                     /locus_tag="PLK64_03795"
     CDS             complement(7347..8576)
                     /locus_tag="PLK64_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HPV78893.1"
                     /translation="MVLGRERGSVTTIVAALFGFMVILGATSLSIDVGSLMWERRQLQ
                     AGADAASLAMARLCALNANECQSAAMTAAAGSGMSIRDLANTTANDGTNGYSTTYVNG
                     VCGNAPAGSDIPECAGSALADLSLCPPVSPDTPESANWIEVHTRTRMNDGSTVLPPFV
                     AQTLGFGGQTLETCARAAWGPAKLSGGNIPFTVSACEWENAGGNVDGSGAGALPPPPY
                     GGTTPWPSSSQEETLWVNVPASRRNPAPCPNFQGHDQPGGFGFLETTSDPCVVRSFGN
                     GDWYHTDPGGSVACDLSRLVGTVVTIPIFSCTLDDVPAGGGAPPPAEPCYDSANGTHA
                     YYYAQSYGRFFLSGYDVVTTGGIPNKVRSVLTGRFPVCPGGQSCITGWFVTGVGDGGI
                     VEGGGGTFGQVGVQAAG"
     gene            complement(8606..9019)
                     /locus_tag="PLK64_03800"
     CDS             complement(8606..9019)
                     /locus_tag="PLK64_03800"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HPV78894.1"
                     /translation="MNSRPLGADRGASAVELAIVLPVLLLVLGGIIDFGRAFTTQMAV
                     TQAAREGARMVALNYSAGDVNSRVNAAAGTFAVTTTIVAGCPARGTTITTQASSVRVD
                     NSFSFLILDSVMNLFGGGLAPTLTLSSTGSMRCMG"
     gene            complement(9272..9430)
                     /locus_tag="PLK64_03805"
     CDS             complement(9272..9430)
                     /locus_tag="PLK64_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HPV78895.1"
                     /translation="MLKALARIRRDHGATMVEYGLMVALIAVIVVVAVGPLGTAISTM
                     FLNVTASI"
     gene            9627..10352
                     /locus_tag="PLK64_03810"
     CDS             9627..10352
                     /locus_tag="PLK64_03810"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPV78896.1"
                     /translation="MPLIPPLEPTTAVPPGPQRIDWGRLVLVPVAVGLLIVNVRAIAT
                     RPWAEPTGLDSTLGLIAQIAVSAFWALVVTAYLRRRPAIATNSSPVTYVVAVTASFLP
                     FVFPLLGERSTNLAVVAVADLLLIVGTAFAAWGIRHLDRSFSILPQARAVVTTGPYRL
                     VRHPLYAGELLAALGLTLLAATVWAFLAWLALIPLQLYRAQHEERILADHLDGYAAYA
                     AQTGQFFPGVGKRKGSHLGRSER"
     gene            complement(10360..12300)
                     /locus_tag="PLK64_03815"
     CDS             complement(10360..12300)
                     /locus_tag="PLK64_03815"
                     /inference="COORDINATES: protein motif:HMM:NF022113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HPV78897.1"
                     /translation="MRPRRGYAGPRGIPTGAEAPRHRRRPLTILWGVLGVLVAVGACS
                     GLPTSSPVQSGRSVNEQVAPEVRIVVPPPAAGASPEQIVRGFLRAGAAFQESTDSGQP
                     VANAYLAPGSVGRWRPTSSVTVFDRSRTVAVEALTGDKVRASVAVVATVDDAGHYREV
                     APGTLAQVDLGLVRVSGEWRVELPADGFGLWLNTDDFGRVFDPHRVHYPTVGGRHLVP
                     DVRWFAGGPRLGTALARAQLGPVPDYLAGAVETGFPADVTLAVDAVSVDAGIATVVLT
                     PPASAMDLAHRRAIWAQLVATLLRVPGVQGVVVEVQGAGRLVVPEVTGPLRSATDVGY
                     GADPLPLPRSALLRLGERLTSVEIARLDDLDGLGTAPTATAPVIPSASAAGGPAPAPN
                     PPATGADLPTIPAAYGQFAVSADGADLVAVAPGGAEAARWHDRERASVPVLTSFVRPS
                     YDALDRLWFAGSDGGVARVLTVNARAPADPAVPVVAPWLADRAIVALAVSRDGSRVGV
                     VTSAPGGSDMRLDIAGVVRDSLGRPTALAAPYRQADPLSRITDVVWIDDTTLAALGAV
                     GDGEALRPWIVQLGQGIGLRRIGIADPATNLIPVAAGARYLVSTSGPRGIVIVAQPGG
                     VLVRVGGSWRGVEGATEIAVAP"
     gene            complement(12297..13919)
                     /gene="mtrB"
                     /locus_tag="PLK64_03820"
     CDS             complement(12297..13919)
                     /gene="mtrB"
                     /locus_tag="PLK64_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrAB system histidine kinase MtrB"
                     /protein_id="HPV78898.1"
                     /translation="MLGPVRPQARNARVVGASAISRVVHAWRRSLQFRVVTTTMLLGM
                     LVLGAVGSYLYQRIGDRLVQGRADLATAEAVQLTVTAQAKFYSTDKTQNTDALTIAAQ
                     SIIGEIAGTERSRYVVLSRLSTNVSSFIVNPVVSGEVNASYIPEALETAVAGDAANQK
                     VQIVSIPLSGEAEPVPCLVIGQQVDLPVARGYGIYYVYPLQRERATLEVISQIFLSGG
                     LVLVLLIGAVAYVVTRLVVTPVRQAALVAERLAAGRLTERMVSRGEDDLASLATSFNA
                     MAEGLQRQIRQLEDLSRVQQRFVSDVSHELRTPLTTIRMAGDLIYDSRTQFDPVVARS
                     AELLRGELDRFEALLADLLEVSRFDAGAAVLDTEPTDLRRTVAKVVDGCQQLAERKGS
                     RITVSDPGVPCEATYDPRRVERVLRNLVVNAIEHGEGKPVEIRVGTNDDAVAVVVRDH
                     GVGLRPGESGLVFNRFWRADPARARTTGGTGLGLAIALEDARLHSGWLQAWGAPGDGS
                     SFRLTLPRAPGTPIGSSPLPLKPVAAVTEAAG"
BASE COUNT         2479 a         4726 c         4508 g         2230 t
ORIGIN      
        1 cgtcgaggtc gaactcatcg tcgcggcgcc gcacttccag ttcacgatca gccactcggg
       61 cgcaacgtcc ctgtcggtgg ccgcgttgcg ggaacgactc gacgacatcg cctgccaact
      121 ctcggtcggc gagcggcgcc ccgagaaggg cggcggggtc caggtgaaag ccgggacgat
      181 cgcccgcttg aaccgtgagt ccgactacgg cctccgtccg cacccgtcgc gatgggaacc
      241 tgcgcccgca accgtttccg aggagggcag ccgatgacaa caccgctcac acgtgcggcc
      301 gcaatcacag cggcaccgcc cggcaccagg aatcaggtca tctcagtcgt gctcatcgac
      361 gaccacgacc tggtccgcca gggcatcgcc tcgttgttgt cgctggaccg cagcttgcac
      421 gtcgtcgccc agggtgcgtc cctggccgaa gggatggtgc tcgctcgatc gatcgtggcc
      481 gacgtcgcgg tcatcgacgt ctcgctgggc gatggttccg ggcttgacct ggtggccgag
      541 ttccgccgcc gccacccggc gatggggttg gtcgtcctca ccatgcatca agacgacgac
      601 accctgctgc acgcgttgga tgccggcgca tccgccctca tccacaagag tcgatcggcc
      661 gaggacgtga tcgccatcgt ccatcaagcg gccgcgtccc ctgaacgctt ctcggccgtg
      721 ggactggccg acgcgatcag acgccgatcc tcagtcgcaa cagtcagatt gacgactcgg
      781 gagagcgaag tcctgcggga gttggcggca ggtgcgtctg ttgcccaaat cgccagggca
      841 ctgtatatga gtgagtccac cgtcaagacc cacatcgcca agctctatga caagctcggg
      901 gccaacaacc gggcgtccgc ggtgatgtcg gcgatgaggc tgggcctcat ccccgcaccg
      961 tgacccttaa ggttcgcgtt ggctctggga tgccccgcgt gcgatgcccc tgcggacgga
     1021 ctggttcaca agtcgccctg gttcacaagt cgccctggtt cacaagtcgc cggagaagaa
     1081 cacgccgatg atccggatga cggccggacc gaggatgacg acgaagatcg acgggaagat
     1141 gcacaggagc accgggaaga tgatcttcac gggcactttc tgagcccttt cctcggcatg
     1201 ttgccgtcgg gcgacgcgca tctccttggc ctgctcgcgc aaaacgttgg cgatcggtag
     1261 tccgagccga tccgcctgga ccagggcgct gacgaacgtc cggatctcgg ggaccttggt
     1321 gcgttctccc attgcggcga acgcatcggc tcgggaccgg ccgatctgaa tctcggacaa
     1381 cacacgagca aactcgccag ccaccgggcc tcgcaccgag cgagcgacct gcatgagagc
     1441 agcgttgaaa ccctgaccgg cctcgacgca cacagtcagc atgtcgagag catcggccaa
     1501 cccacggcgc atctcttcct ggcgtcgcag ccccatgttg tagacgagga ggtccgggag
     1561 gaagaagatc cccgccgcca ggacaagaca ccagatcaaa cctcggagcg agaacccgcc
     1621 gatgagcagc ccgagcagaa ggcccccaat gagacccgcg cccttggcac ccatcagact
     1681 ttcggtggtc cacccggacg ggttgcccgc ctcgtcgagt agcctcgcca ggcgttgggt
     1741 ggcgccgctg ggcgagagcc gctcaccaag gttccgggtg cggtgcagga tcggcacgag
     1801 caaccgatcg cgggccccca actcgttgcg agcaacctca tgtcggtcaa tacgccgctc
     1861 gacgatcgcc aaactgcggg cgacgcctgt cggctcgctg cgtgaggtcg ccgcgacata
     1921 aacgaggaca ccgagggcaa ggcacaccaa ggtgatggcg aacaccaacc atgccatctc
     1981 agacctccac cttgactgcc ttgctcatcc agaagacccc gatggccatc aggatgactg
     2041 cgccgacgag catgatccat cccaggactg tcgtccataa caacgagata tacgaacggt
     2101 tgacgttgac ttcgtagacg aacagcgcaa tcggcagggc aatgaggatg taggccgaca
     2161 gccggccctc ggccgacaaa gcccggacgt gacgacggag gtactcgcgc tctcgaagcg
     2221 tcgccgccgt ggtgcggagc gtctcggcga gattgcctcc gacttgacgt tggataccga
     2281 tcgccatcga cgtccacctc atgttgtcgc tgtccattcg gacagccatg cgttccaggg
     2341 cactctccag atcagatccg atgcgggtct cggcgatcac ccgggcgaac tctttagccg
     2401 caggctctga gacgtctttg gccacggcgt ccatagcctg cggcagggag aaacctgtcg
     2461 acaggctcga cgcaagcagc aacaggatgt cgggcagctg acgttcaagt ttcgcggctc
     2521 ggcgacgggc aagcagtcgc aaggagacgg aggggagaac gagcccagcc agtgccccga
     2581 cgactatgcc gaagacggcc accagaccgt ctcggaacac gagcgcacct gcgatcacac
     2641 cgacaatgac gccgactagc cgcagaaccg cccattcacc gggccgccag gcgaggtcgg
     2701 ctcgctgcag aagtgccatt gtttgggagg tggactcgcg gccggccatc acgcgttcgc
     2761 cgatgtccac caagttggct gtgatcacac tttgggtgtc ccggcgcggc ttggtgatct
     2821 ggagtcctct ggcggagtag gcctcgagat tctggatgcg attctctcgt ttggtccgca
     2881 tggtgggcat cacggctgct gccgtaaggc cgagcagccc taggaaggtg ccggcgatcc
     2941 cgaggacgag gaaccatttg gcgacggttt caacgggtgc tgccaccgcg gcgctcgcct
     3001 caggaagggc aggcgccagg gtcagggttg gcagtgccgt gggcaccgcc gacacggtga
     3061 ccgtgccggt agcagaaaat ggtgttcccg aagccatccc acggacctcg accggtgtac
     3121 tgcccagcgg caacacccca ggacgcacgc tccacatgac ctggcggtcc agggcgcggg
     3181 ctgaggcatt gaaggccccg gtgacagcag cctggtcgtc tgcgggtgcg aaattgccac
     3241 cgccggccgc cgccagcttg atgaggacct cgtcggctgt gaccttcgtg cgcaacccga
     3301 tggtgtcgac cctgacgccg ccttgtgtca ccgcgttgag gacagccgcc aagtctttgc
     3361 ggccagcggt gttcaccccg tcgctgagga cgactatgct ccgctcccct gtcgccccga
     3421 gcttctcaag cgccagggtt accccgtcga agagagccgt ctcaccaccg gcgatgagcg
     3481 tatccgtggc cgtccggatt gctgctttgt ccctcgtgag gtccacatcg acgccagcga
     3541 cgttggcaaa tgagatcacc ccgacgctga catccgctgg gacacggttg agaaactccg
     3601 cgacggccgc cctaattatc ggcattccgg agttagcctt catcgagcca ctggtatcga
     3661 cgacaagaaa gacggcccgc cgcatggtgg tggctgcttc tacggtagcc gccgttgtcg
     3721 cccccgccac ggtcacgtgc agcgatgccg ggtcaactac gaggtcaccg gtggtagccc
     3781 gaagggtgag gacgcccgcg agggtgctcc cgtcgtaagc aagctgtgtc aatgttgccg
     3841 gggcaggctc ggtgtccgcg ttgcccgggg gggcgaagag cagggtggca gtgaagccag
     3901 ccgctagcgc gcgcagcaca cgcgagcgac gaggccccct ccgcggagca cgaactcggt
     3961 ccgtcatcgg ttccctcgga cgaacatcgt ttgaggcagg acgatgcctt ggtcagccag
     4021 gacgtcaagg aacttcggtc ggagtccggt gctgaccaag cccccgaggt acctgccgtt
     4081 ctcgtcgcgc ccggccttgt agtcgaagct gaagatgtcc tgcagggtga tgatgtcgcc
     4141 ctccattccc tcgacctcca cgatggaaac gacgcgacgg ctgccgtcgc gcaagcgcgc
     4201 ctgctggatg acgagatcga ccgcactggc gacctgatcg cgaatggccc ggagtggaag
     4261 ttcgaccccc gccatgagga ccatggtctc cagccggctc aggctgtctc gcggggagtt
     4321 ggcgtggaca gtggtgatcg acccgtcgtg gccagtgttc atggcctgca acatgtccag
     4381 tgccgcgccg tctcgcactt ctccgacgat tattcggtcg gggcgcattc gcagcgagtt
     4441 tcgaacgaga tctcggatcg cgatctgccc gcggccctcg atgttggacg ggcgcgactc
     4501 cagtcgcaga acgtgctgct gatggagttg gagttccgcc gcgtcctcga tggtgatgat
     4561 ccgttcgtca ttggacacat acgacgacag gacgttgagg gttgtggtct ttcctgaccc
     4621 cgtccctccc gagatgatga tgttgcggcg cccgcggacg caggccgaga ggaactcggc
     4681 aacagactcg ctgatggtgc cgaacgcgat gaggtcgcgg gcggtgaacg ggtcggcggc
     4741 gaactttcgg atcgtcagca tcgatccgtc gagcgccact ggcggaatga cagcgttgac
     4801 ccgcgatccg tcaggcagtc gcgagtcgac catgggactg gcctcgtcga cccgccgacc
     4861 gatccgtccg acgatcttgt cgatggtcct ccgaaggtgc gcctcgctgg tgaacttcgc
     4921 gtccacccga tatatccgac cggagcgctc aacgtatatg ttctcgtgcc cgttgaccat
     4981 gacttcggtg acgtcgttgt cgcgtagata gggctccagt ggcccatggc cgagaatctc
     5041 gtcggaaacc tcttgcgcaa tgcgggttcg gtcagcggcc gagagcggag tctgctgccc
     5101 gtcgatgaca acttgcaggg tctgtcgtac tcgctggtcg agatcgacct gcccaagatt
     5161 cgggtcgtac agctgagggc cgagcgtgtc taggagggtc tggtgaactg ccgccttcac
     5221 cgcagcgaac gggtcgaggc tggcgcgcgc gggtgcatgt ggtcgggccg gagcatcccc
     5281 caacctcgcg ccggttgtcg gtgtttgtgt cctgcgaaca gtctcaagcc ggtcgtagag
     5341 actcacttgc gtcctcccca tggaagcaac cgccgcggag cagcagactg gctcgccgcc
     5401 ggatcgacgc cgcgaatgtg ttggtcggcg aggtgacgaa gtgccacgct caccgggtga
     5461 cgggggtcat ccaagatgat gggcacccct cggttgatcg aggccgggac gtctgggctg
     5521 cttggcacca tgacggcaat ctcctgctta atggacgtca cgacatcctc gacccgcagg
     5581 cccacctttg cgtcggaccg gttcagcacg atatgccggg cttcccgggg gctccccaag
     5641 aggtcaaggg tgtccagtgt gagccgcagg ttcttcaatg ccggaatatc caacgtggcg
     5701 ataaggacaa ggctgtcgct gagatcaaag gcggtaagaa cgtgttcagt cagggccggt
     5761 ggcgtgtcga taaccacaaa gtcatacaca tagcgtgcaa cccggagcaa ctcagcgaca
     5821 gtggccggcg ggatccgctc ggcctcaccg ggctcagccg gggcacacac cgcctcaagc
     5881 ccactggaat gtatggtgac aacggaagct aggccttgct cgtcgaggtg tcccgtcatg
     5941 ttgacaacat cggtcatgct gcgctctgga agcatttgca gcgcgatccc gacatcaccg
     6001 aattccaggt cgagatcgac gacgagcgtg cggtgaccga ccgaggcgag ataggcacca
     6061 aggtttgtcg agaacgtcgt cttgcctacg cctcccttgg cggagaagac ggtgacgatc
     6121 ttccccgccc gggaagctga gtccgcgctg acaccgcgca gacggaagct gagttcccgg
     6181 ctgcggcgca gcgcatcggc cacggcagga aggtcgtcgg cggacaccac ttcgcgaaac
     6241 cccgcgcgaa gtgcctgggc caggatgttc acatcgagac ggcgtcgcac caggatcaca
     6301 ccgagcgcag tgtgctcaac gcgcagaccc gcaaccacac cacaagcaga gtcgaggtcc
     6361 acatccggtc cgacgatcac cgcgtcctca tcaggggacg cgctcagcgc gcgactcaag
     6421 gccgcgccgc tgtcaacccg agtcgcgttg ggaccaagcg cgaaactgaa ccgctcagca
     6481 gacgctggat cccgatccca gaggatcgtc acggcgcgac ctcgaacacg ttgtagtcgg
     6541 tgatctgcac ggtcggatca attgtcgtgt cggctccgcg cagaccggca tacaaggcgc
     6601 ccgtctgtat gccgtgtacg agccggttgg cgtcgagcgg cgtcagagcc agagtgacga
     6661 ggaacgtggg cttggtctga tcctgcgcgc cattctgggt attcgtgtcc gcgggcgtga
     6721 gcgacgtatc gcccatggcg atgacaaggg cgtcaccaag gaggacacgc gtcgcttgag
     6781 tttgggttgg gtttcccgcc gtgggcgtcg ggctcgaggt gggagcgggc atctcgaagg
     6841 tatcgaagat gacgatccgc gaccccgggg tgacgaaact ccccacccga gctgggtctt
     6901 ccagccggac cgacacggcg acctgccccg gcggaacttg gatggccctt cgcccggtcg
     6961 gggtcgtccc gaaccgggac gccaacacgt actcacccac accgatgtcg gtgatcgcaa
     7021 caagcgcttc gttcgtcggc gtcacctcgg ccaacgcgcc caccggaaag gctcgtgccg
     7081 cgacctgcgt cttgaccaac gcacgatctc ggaccgcatc ggccagtgac gtccccgccg
     7141 agaccggctt ctccgacacg tacaccgtgc gggggtcggc gcccgcaacg gcacgagcat
     7201 ccgccccccg cgcgtaaatc aacacggcag cggcgccgag caccgcgata aggcccgcca
     7261 acaacaccgc tattgcacgt cgtcccacaa gaacacatcc tgctcaataa tcgggaggtt
     7321 gccctcactg accggaacgg cttcgattac ccggctgcct gaacacccac ctgaccgaaa
     7381 gtccctccac caccctcgac aatccctccg tcgcccacac ccgtcacgaa ccaacccgtg
     7441 atgcaactct ggcctcctgg acaaactggg aaacggccag tgaggacgct cctgacctta
     7501 ttggggatcc cccccgtggt tacgacgtcg tagccagaaa ggaagaaccg gccgtagctc
     7561 tgggcgtagt aataggcgtg agtaccattc gctgagtcgt agcagggttc agccggtggg
     7621 ggcgcgccgc ccccagccgg cacatcatcg agggtgcagc tgaaaatcgg aatagtgaca
     7681 acggtcccga ccagcctgga caaatcgcac gcgacgctcc cccccgggtc ggtgtgatac
     7741 cagtcgccgt tcccgaagct cctcacgacg cacgggtcgg acgttgtctc caagaagccg
     7801 aacccaccgg gttggtcgtg gccctggaag tttgggcacg gcgccggatt cctcctgctg
     7861 gccggaacgt tcacccaaag ggtctcttct tgagacgaac tcggccaagg ggtggtaccg
     7921 ccgtacgggg gaggaggcag agcacccgcg ccgctcccgt cgacgtttcc acccgcgttc
     7981 tcccattcac aggccgagac cgtgaagggg atgttcccgc cactcagttt cgccggcccc
     8041 caggccgccc tggcacacgt ctccagggtc tgcccgccaa accccagcgt ctgtgcgacg
     8101 aacggtggga ggacggtcga gccgtcgttc atccgggttc gcgtgtggac ttcgatccag
     8161 ttggcgctct ccggcgtgtc tggggagacg ggcgggcaga gggaaaggtc agcgagcgca
     8221 ctgcccgcac actccgggat gtcgctgccc gcaggtgcgt tcccgcacac cccgttgacg
     8281 tacgtcgtgg aatacccgtt ggtgccgtcg tttgctgtcg tattggccag gtcgcgaatg
     8341 ctcatgcctg agcctgcggc agcagtcatc gccgcactct ggcactcgtt cgcgttgagg
     8401 gcgcacagtc tcgccatcgc caagctggcg gcatcggctc cggcctgaag ttgacggcgt
     8461 tcccacatca acgaaccgac gtcgatcgac agggaggtcg cgccgaggat gaccatgaaa
     8521 ccgaacaggg ccgcgacgat cgtcgtgacc gatccacgct cccgccccag caccatgcgg
     8581 cggcgaccct tcctcgtcac atgcgtcaac ccatgcatcg catgctccca gtcgaagaaa
     8641 gagtcaaggt cggcgccagc ccgccgccaa agaggttcat cacactgtcg agaatgagga
     8701 aactaaagct attgtcgact cgcaccgagg acgcttgggt ggtgatcgtg gtccccctcg
     8761 ccgggcatcc ggcgacgata gtggtcgtca ctgcaaacgt ccccgcggct gcgttgacgc
     8821 ggctattgac gtcgccagcg gagtagttga gcgccaccat tcgggcgcct tcgcgtgcag
     8881 cttgggtcac ggccatctgc gtagtgaaag ctcggccgaa atcgataatg cccccaagga
     8941 cgagcagcaa cacgggaagg acgatggcaa gctcgacggc cgaggcgccc cgatcagccc
     9001 ccagtgggcg cgagttcacg cattccctcc tacaggcctg ccggccagcc ggtgaagagt
     9061 cccgacactg ccgacccgaa ttgagtcacc gcgaatgctg aaacggctgc gatgaacgct
     9121 gctaggatca cgtactccgc catggtggcg ccgccatcgc gctgtctcag acgaagaccc
     9181 cccaaggttc tcagccttcc gcgagcgtaa ccgagcccga acgcgggagg gatggtttca
     9241 tcgaaaccgc ccctccccgc ccagccgccg ctcagatact ggcggtgacg ttcaggaaca
     9301 tcgtcgagat ggccgtgccc agcggaccga ccgcaacgac gacgatgacg gcgatgagtg
     9361 cgaccatcaa gccgtactcc accatggtgg cgccgtggtc ccggcggatc cttgccagtg
     9421 ccttgagcat gggggttccc tcccaaaatc gtgatggata ccggacggtt ccggtcctac
     9481 cggcatcatc cgcgcgtgct actggccctc gaatccaccg accatctcat ccctcctcgt
     9541 ccgcacggct tcccctgcct catccgaacg gacgatcctg gggacgccgc cctcgcctgc
     9601 gggtcccgat gagtcaccat gctcgaatgc cccttatccc accgctcgaa ccgacgacgg
     9661 cggttccccc gggaccacaa cggattgact gggggcggct ggtcctcgtg cccgtcgccg
     9721 ttgggctact catcgtcaac gtcagggcga tcgccacgcg cccatgggca gagccgactg
     9781 ggctcgactc gacccttggc ctgatcgccc agatcgccgt cagcgccttc tgggccctcg
     9841 tcgtcacggc gtacctgcgg cgtcggccgg caattgcgac caactcctcg cccgtgacct
     9901 acgtggtagc cgtcaccgcg tcgtttctcc cgttcgtctt tcccctgttg ggggagcgct
     9961 cgacgaacct cgctgtcgtt gcggtcgccg acctcctcct catcgtcgga acggccttcg
    10021 ccgcatgggg gattcgccac ctcgaccgca gtttcagcat cctcccgcag gcgcgcgcgg
    10081 tcgtcacgac tgggccgtat cgcctggtcc gccatcccct gtatgccggg gagcttctcg
    10141 cagccctcgg tttgaccctg ctcgccgcaa ccgtatgggc cttcctcgcg tggctcgcct
    10201 tgatcccctt gcagctctac cgggcccaac acgaggagcg catcctcgcg gaccacctag
    10261 acggctacgc ggcatacgcc gcgcaaacgg gacagttctt ccccggcgtc ggcaagcgca
    10321 agggctcaca cctgggacgc tccgagcggt agctcatccc tagggggcga ccgcgatctc
    10381 agtggcccct tcgacccccc gccacgaccc gccgacccgc acgaggacac caccgggttg
    10441 ggcgacgatg acgatccctc gtggaccact ggtcgacacc agataacgag ccccggccgc
    10501 cacggggatg aggttggtgg cagggtcggc gatcccgatg cgccgcagcc cgattccctg
    10561 accgagttgc acgatccacg ggcgcagggc ctcgccgtca ccaaccgcgc cgagcgccgc
    10621 cagagtcgtg tcgtcgatcc acacgacatc ggtgatccgg ctcagggggt cagcttgccg
    10681 atacggcgcg gcgagggctg tcggccggcc cagggagtcc cgcacgaccc cggcgatgtc
    10741 cagtcgcatg tcgctgcccc cgggggcgct agtgacgaca ccaacccggc tgccgtcgcg
    10801 ggacacggcc aacgcgacga tggcgcgatc agccagccac ggcgcgacca cgggcacggc
    10861 cggatcagcc ggcgcccgtg cattgaccgt cagcacccgg gccacgcccc cgtcgctccc
    10921 ggcgaaccac agccggtcga gcgcgtcgta ggagggccgc acgaacgacg tcagcaccgg
    10981 cacgctcgcg cgctcccggt cgtgccaccg agcggcttct gccccgccgg gagcgacggc
    11041 gacgaggtcg gctccgtcgg cggagaccgc gaactggccg tatgccgcgg gaatcgtcgg
    11101 gaggtcggca cctgtggccg gcgggttcgg tgccggggcg ggcccgccgg cggcactggc
    11161 cgaggggata acgggggccg tggccgtggg agccgtgccc aggccgtcga gatcgtccag
    11221 ccgtgcgatc tcgaccgacg tcagtcgctc gccgaggcgc agcagagcgc tgcggggcag
    11281 tggaagggga tcggcgccgt aaccgacgtc agtggccgac cgcagcggac ccgtcacctc
    11341 gggcacgacc agccgcccgg ccccctgcac ctcgacgacc actccctgga ctccaggcac
    11401 gcgcagcaac gtagcgacga gttgggccca gatggcgcgc cgatgggcta ggtccatggc
    11461 gcttgccgga ggggtcaaca cgacggtcgc gatccccgca tcaacgctca cggcatcgac
    11521 ggccagcgtc acgtcggccg ggaaccccgt ctcgaccgct ccggcgaggt agtcgggaac
    11581 gggaccgagc tgggcacggg ccagcgcggt ccccagccgg ggcccacccg cgaaccacct
    11641 gacgtcgggc acgaggtgcc gcccgcccac cgtcgggtag tggacccgat gcgggtcgaa
    11701 gacccgcccg aagtcgtccg tgttgagcca aagcccgaac ccatcggcag gcagctcgac
    11761 ccgccactcc cccgacaccc ggaccaggcc caggtcgacc tgggcgagcg tccccggcgc
    11821 gacctcgcga tagtgccccg catcgtcgac ggtcgcgacg accgcgacgc tggcgcgaac
    11881 cttgtcaccg gtcagcgcct cgacggcaac tgtcctggat cggtcgaaca ccgtcaccga
    11941 actcgtcgga cgccaacgac ccaccgagcc tggggcaaga taggcgttgg cgacgggttg
    12001 gccgctgtca gtgctctcct ggaaggcggc gcccgcacgc aggaagccac ggacgatctg
    12061 ctccgggctc gcgccggcgg caggcggtgg cacgacgatc cgcacctcgg gcgccacctg
    12121 ttcgttgacg ctccgaccgg actgaaccgg gctgctggtc ggcaggcccg agcacgcccc
    12181 gactgccacc aggacaccga ggacacccca caggatcgtg agcggccgcc gtcgatgccg
    12241 cggagcctcc gcacccgtcg gtatgccgcg cggaccggca taccctcggc gcggcctcac
    12301 cccgccgcct cggtgacggc cgccacgggt ttgagcggca acggggagga cccgatcggt
    12361 gtcccgggag cccgcggcag ggtgagccgg aaggacgagc cgtcgcccgg agcgccccac
    12421 gcctgcagcc agcctgagtg cagccgggca tcctcgaggg cgatggccag acccaggccg
    12481 gtgccgccgg tggtgcgggc ccgggccggg tcggcccgcc agaagcggtt gaagaccaga
    12541 ccggactccc cgggccgcag gccgacgccg tgatcgcgca cgacgaccgc gaccgcgtca
    12601 tcgttggtgc cgacccggat ctcgaccggc ttgccctcgc cgtgctcgat cgcattgacc
    12661 accaggttgc gcagcacccg ctcgacccgg cgagggtcgt aggtggcctc gcaaggaaca
    12721 ccgggatcgc tgacggtgat ccggctgccc ttgcgttcgg ccagttgttg gcagccgtcg
    12781 acgaccttgg ccaccgtgcg gcgaaggtcg gtcggctcgg tgtcgagcac tgcggcgccg
    12841 gcgtcgaagc ggctcacctc cagcaggtcg gccagcagcg cttcaaagcg gtcgagctct
    12901 ccccgcagca gttcggcgga gcgggcgacc accgggtcga actgggtccg ggaatcgtag
    12961 atgagatcgc cggccattcg gatcgtcgtc agtggcgtgc gcaactcgtg ggagacgtcc
    13021 gagacgaagc gttgctgtac ccgcgagaga tcctcaagct ggcggatctg acgttggagg
    13081 ccctcggcca ttgcattgaa ggaggtggcc aggctggcga ggtcgtcctc tccgcgagag
    13141 accatccgct cggtcaggcg gccggcggcc aaccgctcgg ccacgagcgc cgcctgccgg
    13201 acgggggtga cgaccaggcg cgtcacgacg taagccacgg caccgatgag caggacgaga
    13261 acgagcccac cggagaggaa gatctgcgag atgacctcga gggtcgcccg ttcccgttgc
    13321 agtgggtaga cgtagtagat cccgtagccg cgggcgaccg ggaggtcgac ctgttgcccg
    13381 atgaccaagc agggaaccgg ctcggcctcg ccagacaacg ggatcgagac gatttgaacc
    13441 ttttgattgg ctgcatctcc ggcgacggcc gtctccaacg cctcggggat gtaggaggca
    13501 ttcacctctc cggagacgac cgggttgacg ataaaactgc tgacgttcgt cgacagacgg
    13561 gacaagacga cgtatcggga acgctcggtt cctgcgattt cgccgatgat cgactgcgcg
    13621 gcgatggtga gggcgtcggt gttctgggtt ttgtctgtcg aatagaactt cgcctgggcg
    13681 gtcaccgtca gctgcaccgc ctcggccgtg gccaggtcgg cccggccctg gacgagccga
    13741 tccccgatgc gctgatagag gtaggacccc accgcaccca gcacgagcat gcccaggagc
    13801 atcgtcgtcg tcacgacgcg gaactgcagc gacctccgcc atgcatgcac gacccgcgat
    13861 atcgccgacg ctccgacgac tcgagcattg cgcgcctgcg gccggaccgg cccgagcaca
    13921 gattcaccga gtgatcccgg ggg
//
LOCUS       DAOMZY010000040        13793 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_4068_length_13793_cov_5.482457, whole genome shotgun sequence.
ACCESSION   DAOMZY010000040 DAOMZY010000000
VERSION     DAOMZY010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 13793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13793
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4068_length_13793_cov_5.482457"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..695)
                     /locus_tag="PLK64_03825"
     CDS             complement(111..695)
                     /locus_tag="PLK64_03825"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF-related protein"
                     /protein_id="HPV78899.1"
                     /translation="MTSDVPQDSPTWPPAPNPANVGGAAYSPVPQPGALQDASPDASA
                     TDRVWTIVGSTKRRGDWVVPERLVLPVALGDVVIDLRQARFTAATTTIEVQGLMGDVK
                     IIVPDTIRVECSGSAIIGEFKDVPPEVTTTPAQSAAVVRVVGAMIMGQVRVFRTADAV
                     GEARYAIDGMAGWRAQRRRRRDRRRLKGRQGIAD"
     gene            complement(844..1470)
                     /locus_tag="PLK64_03830"
     CDS             complement(844..1470)
                     /locus_tag="PLK64_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78900.1"
                     /translation="MPPALGVLALWGSRGHLSASGHIFADLHRLSVARRTVAGLLAGV
                     VSFGMSGCGASDFTSSSLATLVPTSSQPVVTTKAKAKATATATATAASTSTLGVLPPV
                     LSTSSSPVDAAVVTSLADLPAAFGCPKVPQPIVVPASGDKPPALVCRSELAEEALFLW
                     YVDDADSKYRAVKAALATARYVRAGRHWVAGGMVDKTMGSVGGDVFKQ"
     gene            1519..2724
                     /locus_tag="PLK64_03835"
     CDS             1519..2724
                     /locus_tag="PLK64_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HPV78901.1"
                     /translation="MRAQSVSPDAPDVGHLLDLTDPDVVADPYAALAACRATAPVGWH
                     GPLGMYVATSHAACNAVLRDRALGRIFTAREPANAWDTFNWLHADSILDSEPPKHTRL
                     RRLIAGAFGRGHVHRLAPRIEELAEGLVADCAATVSEEGAVDVIEALAEPLPVLVIAE
                     LLGVPEPDRHLLRPWSQAIVRMYEVTRTREEDEAAQLACREFAAYVEALAADRARHPG
                     EDLLSDLVAARDGTDRLSPQELVATAVLLLNAGHEASVNGFGNGLASLLAAGGDFGDP
                     VRLVEEMLRHDSPLHLFERTATHDTVIEGVHIRAGEKVAALLGAANRDPAVFAEPDAF
                     RPDRDPNPHLAFGAGIHFCVGAPLARLELATAAGVLLGRWPRLRVVDAVRRPTFVLRG
                     YERLVIAPA"
     gene            complement(2694..3656)
                     /locus_tag="PLK64_03840"
     CDS             complement(2694..3656)
                     /locus_tag="PLK64_03840"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPV78902.1"
                     /translation="MGKIGWVHAWQEALYGPRGFYRGQVPARDFATATHPPLGAVLAE
                     AIWHYADLLELSGVVDIGAGRGELIGALHSHRPDRPLLGVDVVERPADLPDGVGWLRG
                     PGGAPLPDELSDLTDVLVVAHEWLDVVPCVIGQLDADGVVREVLVDPTSGDEDLGDPL
                     ADAELAWQQRHWPLLAEGDRVEVGLSRDLAWADLLSRVCRGAAIAIDSGHVAAARPRN
                     GTLAAYRGDAAVRPVPDGSCDLSAQVAVDSLHHDEIHTQTTAFESFGVWAPMPDPALA
                     RCDETAYRRRLLRATSAATLTDPAGHGAFRWVVVRQAGAMTKRS"
     gene            3711..4484
                     /locus_tag="PLK64_03845"
     CDS             3711..4484
                     /locus_tag="PLK64_03845"
                     /inference="COORDINATES: protein motif:HMM:NF022187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HPV78903.1"
                     /translation="MSTPSGPTARLRVGVIGAGRAGVALGAALARAGHLPVAVAAVSD
                     ASRERAEAVLPGVPIREALDVVAEADLVLLAVPDDVLPGLVAGLAAAGAFHPGQVLLH
                     TSARYGIGVLEPAATHDVLPVAIHPAVALTGELGDGERLAAALFAVTTLKSLRPLGEA
                     LVMEMGGEPVWVEEEDRPAYAAALGAVRDGLEGVLGAASRVLADCGIPHPARALAPLV
                     LTALEDALRTGSVARDPGVYAAPVVDRPSDGPEPLEDDQ"
     gene            4481..6205
                     /locus_tag="PLK64_03850"
     CDS             4481..6205
                     /locus_tag="PLK64_03850"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HPV78904.1"
                     /translation="MTPIHIPRWLRAPSPGWSTSADVIVVGSGIAGLTAALRLRARGA
                     TVLLVTKTLLTEGSTAWAQGGIAAALADDDSPGDHLHDTLVAGVGLCDIPAVTALVHE
                     GPTRVRELVTLGAEFDRGSDGAISLTREGGHHRDRILHAGGDATGKEISRALIEALHR
                     VTDDPGIEIIEHALVVDLLQDAGGRVCGATIHVIGEGQIDGVGAAQARAVVLATGGLG
                     QIYSSTTNPPVATGDGMAIALRAGAVMADMEFVQFHPTVLFLGDDSTGQQPLISEAVR
                     GEGAFLVDVDGVRFMQGVHPMADLAPRDVVSRAIVRQMAATGSDHVFLDARHLGAEFL
                     ERRFPSIVASCREHGFDPATTLLPVAPAQHYASGGIRTDLVGRSSLDGLYACGECSCT
                     GVHGANRLASNSLLEGLVFAHRIAEDIAERFAQGELPSVTATAAAPQSDGPDGSGIGE
                     GRMRDTVLLRSVHRKAIQRAMTSGSGVVRSASTLESTASALATLAAEADGDEGGPKSW
                     ETTNLLHLGQLLTQVAALREETRGGHVRSDFVDRDDAHFLGHVRAVRAADGILATSFH
                     PTAPQDLS"
     gene            6202..>7077
                     /gene="nadC"
                     /locus_tag="PLK64_03855"
     CDS             6202..>7077
                     /gene="nadC"
                     /locus_tag="PLK64_03855"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPV78905.1"
                     /translation="MTPDLAFPAADARALVRLALDEDLGPRHVDVTTLSTIPADQERT
                     AHVVARAAGVIAGVPVVAEVFGAVADLIGCATPEVELLVADGTAVVRGDVVARVSGPV
                     RAILIGERTMLNIVSRLSGVATHTRRWADRLDGTGAMVLDTRKTTPGMRHLEKYAVRA
                     GGGTNKRMGLYDVAMIKDNHKIAAGGLAPAYAAVRRTFPEVDIQVEVTTVAEALEAYA
                     AGARFIMCDNMSVALLGETVAALRAQDEPVEIEATGGLTLEIARDYALTGVDYLSVGG
                     LTHSSPILDVALDLVD"
     assembly_gap    7078..7177
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7197..7988)
                     /locus_tag="PLK64_03860"
     CDS             complement(7197..7988)
                     /locus_tag="PLK64_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPV78906.1"
                     /translation="MVVKRSGMPPAHRRGSAADPFPPLPVRRVELRAGSDLPTDIWPA
                     TLAPISQLLREGLDLGPATVLVGANGSGKSTLIEGIAMAFGLAPEGGSTGARHRSYDS
                     ESPLHEALQIVRGPGGSRWGYFVRAETMHALMGYLDATAVDSYTRDPTFHRFSHGESF
                     LALMQTSRFRGEGFFVMDEPEAGLAFEAQLTLVGRLAQLARRRGAQVLVATHSPIIAA
                     LPGAQILELDAEGFRPVTWDELTVVDHYRRFLAAPSQFLRHVLPD"
     gene            complement(8046..9014)
                     /locus_tag="PLK64_03865"
     CDS             complement(8046..9014)
                     /locus_tag="PLK64_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HPV78907.1"
                     /translation="MILRKALLTLPALGAVTLLALGVPAASVASAAAVSPASAASGAA
                     AQACVEHTDSVNDARSGAPGNRKDPQELSDAEVAARESDFQARVLAKSSRSMLPQATA
                     AVATVPINVYWHQITDGTNGVMGSNQISNQISVLNAAYAGSGFSFTLVSTDSTNNAAW
                     YNGLTNGSTAERNMKTALRQGGKAALNIYTADLGNSLLGWATFPKATLDVMDGVVVLD
                     ESLPGGTAAPYNLGDTATHEVGHWLGLYHTFQGGCNGKGDYVADTAAEKSAAFGCPTG
                     RDSCRTKPGLDPITNFMDYTDDACMFQFTPGQGARMQAQWATYRAA"
     gene            9285..10790
                     /gene="lysS"
                     /locus_tag="PLK64_03870"
     CDS             9285..10790
                     /gene="lysS"
                     /locus_tag="PLK64_03870"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019483981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPV78908.1"
                     /translation="MPTDPIGPSDEVDDLPEQLRVRRDKRDRLIASGHAAYPVWVDRT
                     HSLGDLRRDWGHLEAGQETDAVVRTAGRVMYIRNTGKLCFATLQEGAGTRLQVMLSLA
                     EVGEAALADWKALVDLGDHVAVEGRVVSSKRGELSVMATSWQMASKALRPMPTLHKDL
                     SEETRVRQRYADLVVRQEARDMVRLRARATRAIREVLDTQGYLEIETPVLQLVHGGAA
                     ARPFRTHLNAFDQQMTLRIALELNLKKAVVGGVDRVYEMGRIFRNEGVDGTHSPEFTM
                     LEAYQAYGDQTTIAALIRDLYLGVADALGTRVVQTPRGEVDLGGEWRWLPVYDAVSDA
                     VGEEVTLATPVETLRRYAAAHDVPLDPAWGADKVVLELLSELVEPKVLQPTFFCDYPA
                     IAQPLARPHRSVPGLIEAWDLIIAGVERGTGFSELIDPVIQRQVLEAQSLLAAGGDPE
                     AMALDEDFLRALEFGAPPMGGLGLGIDRLVMLFTGASIRETILFPLLKPED"
     gene            10793..10984
                     /locus_tag="PLK64_03875"
     CDS             10793..10984
                     /locus_tag="PLK64_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl-tRNA synthetase"
                     /protein_id="HPV78909.1"
                     /translation="MWDAIYPYLAAILPTIGLLVIAYVVFKTIFEADRRERKAVAEWE
                     RSHDASPISSTDDESRPTT"
     gene            11348..11677
                     /locus_tag="PLK64_03880"
     CDS             11348..11677
                     /locus_tag="PLK64_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsr2 family protein"
                     /protein_id="HPV78910.1"
                     /translation="MAKRVQVVLEDDVDGSPADETVSFGLDGVSYEIDLSAENASALR
                     DQLAKWIGAAQRTGGRRAAGRRAGGSARRTDVSAVRAWARANGHQINERGRVPAAVQA
                     AYDKAHA"
     gene            complement(11741..12643)
                     /gene="ligD"
                     /locus_tag="PLK64_03885"
     CDS             complement(11741..12643)
                     /gene="ligD"
                     /locus_tag="PLK64_03885"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HPV78911.1"
                     /translation="MPEPTDPAKVTVDAGGRRLVVSNLDKVIYPATETTKGEVLHYYA
                     RIAPVLLPHLAGRPVTRVRFPEGVEGLNFFEKNLPSGAPAWLPRVTLTHTSEPITYPM
                     VTGLDALTYLANLASLEFHVPQWQIGADGLPAFPDRLVVDLDPGPGAGLQECSIVALL
                     ARERLRALGLDPIPVTSGSKGMQLYAALPGSHASADIALVARTLAQELTAAHPDLVVW
                     KMTTSLRPGKVFFDWSQNVAAKTTISPYSLRGRPAPFVAAPRTWDEVQEGAATPGALA
                     QVTATEVLDRVERLGDLAAAVLPQ"
     gene            complement(12636..13580)
                     /gene="ligD"
                     /locus_tag="PLK64_03890"
     CDS             complement(12636..13580)
                     /gene="ligD"
                     /locus_tag="PLK64_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HPV78912.1"
                     /translation="MRPMLATPTRRVPTGPEWAHEVKWDGMRILADIHDGQVRLTSRT
                     ERDVTVAFPELAGLANEFEDMLLDGEAVVLRDGIPSFAALAERFHVTSPTKAAALAAR
                     APVTLMVFDLLRLYGVDLTGRSWTDRRATLERLELQNPHWTVPPTFTDGQALWEATRE
                     QGLEGIVSKRRSGRYAAGARTEDWVKLPHRTSQSVVIGGWRPETDQVGRLGAVLVGVP
                     GVGGLRYLGRVGSGIAGKTARALAADLAPLTSAGSPFVDEVPPEDASGTTWVHPVLVC
                     DVQALGLTDSRRLRQPAYRGLRPDLTPGDIDPTELPDA"
     gene            complement(13586..>13793)
                     /locus_tag="PLK64_03895"
     CDS             complement(13586..>13793)
                     /locus_tag="PLK64_03895"
                     /inference="COORDINATES: protein motif:HMM:NF020544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HPV78913.1"
                     /translation="ISAALEALPASEVAARVDFEAMRGADIYPGFWDEQDVFHTWLRP
                     RYKDLRKFYRRAARASSAVLVAIL"
BASE COUNT         2056 a         4698 c         4852 g         2087 t
ORIGIN      
        1 gatttgtcgg atcgacccac ccgacgccgg ggaccccggt gaccgggcga ccaaccctgg
       61 aggcgtggcg cccgcctaac gacccgccag gcgacccggc aggtcggggc tcagtcagcg
      121 atcccctggc ggcccttgag gcgtctgcgg tcccggcgcc gacgccgctg cgcccgccag
      181 ccggccatcc cgtcgatggc gtagcgcgcc tcaccgaccg cgtcggctgt gcggaagacc
      241 cgcacctgtc ccatgatcat cgcgcccacc acgcggacca ccgcggccga ctgcgctggg
      301 gtggtggtca cttcaggcgg aacgtccttg aactcaccga tgatcgccga cccgctgcac
      361 tcgacccgaa tggtgtcggg gacgatgatc ttcacgtcgc ccatgagacc ttgcacctcg
      421 atggtcgtcg ttgccgccgt gaaccgggcc tggcgaaggt cgatgacgac gtcgcccagg
      481 gcgacgggga gcaccaggcg ctccggcacg acccagtcac cgcgccgttt ggtgctgccg
      541 acgatcgtcc agacccgatc cgtggcgctc gcgtctgggc tcgcgtcctg aagggcgccc
      601 ggctggggca ccggagagta tgccgccccg ccgacgttcg cgggattcgg ggcggggggc
      661 caggtgggcg aatcctgcgg cacatcgctc gtcatgaaac accatcctgg gcgttgggtc
      721 ggtgcagcgc catggggtgg acccccgatc gcaccccgag aagggtcagg ggtttaacca
      781 gcctggcgac gacccgcggc gaccggccat gtgcgagcgg cctgagcgac cggccggggc
      841 gggttactgc ttgaagacgt cgcccccgac gctccccatc gtcttgtcca ccatcccgcc
      901 ggccacccag tgccggccgg cgcggacgta gcgggcggtg gccagcgcgg ccttgacggc
      961 ccggtacttc gagtcggcat catcgacgta ccagaggaag agggcctcct cggccagttc
     1021 actgcggcag accagggccg gtggcttgtc gcccgaggcg gggacgacga tcggctgggg
     1081 gaccttgggg cagccgaagg cggcgggcag atcagccagc gaggtgacga cggcggcatc
     1141 gacgggcgac gacgacgtgg agagcacggg tgggaggacg cccagggtgc tggtgctcgc
     1201 ggcggtcgcc gtggccgtgg cggtggcctt ggctttggcc ttggtcgtca ccacgggttg
     1261 cgacgaggtc ggcaccagcg tggccaatga ggaactggtg aagtcgctgg ccccgcatcc
     1321 ggacatgcca aaactgacaa cgcccgcgag aagccccgcc acggttcggc gggcgactga
     1381 caaacgatgc agatcagcga aaatgtgacc ggacgcgctg aggtgccccc tcgagcccca
     1441 gagcgccagc accccgagag ctggcggcat acccaacagt ctgccgcagc gaagggccga
     1501 gccggtaccg tgacgcgcgt gagggcccag tcagtgtcgc cggacgcccc cgacgtgggg
     1561 cacctgctcg acctcaccga ccccgacgtc gtcgccgatc cgtatgccgc cctcgccgct
     1621 tgccgcgcga ccgcgccggt cgggtggcac gggccgttgg ggatgtacgt cgcgacgagc
     1681 catgcggcgt gcaacgcggt gctacgggac cgggcgttgg ggcggatctt cacggctcgc
     1741 gaacccgcta acgcgtggga caccttcaac tggctgcatg ccgactcgat cctcgacagc
     1801 gagccgccga agcacacgcg gctgcgtcgg ctcattgccg gtgccttcgg gcggggccac
     1861 gtgcaccggc tcgctccgcg catcgaagaa ctcgccgagg gactcgtggc cgactgcgcc
     1921 gcaacggtgt ccgaggaggg tgcggtcgac gtcatcgaag cgctcgccga gccgctgccg
     1981 gtgctggtca tcgctgagtt gctcggggtg ccggagcctg accggcacct gttacggccc
     2041 tggtcgcagg ccatcgtccg gatgtatgag gtgactcgga cccgcgagga ggacgaggcg
     2101 gcgcagcttg cctgccgcga gttcgcggca tacgtcgagg cgttggctgc cgaccgggca
     2161 cggcacccgg gagaggactt gctctcggat ctcgtggccg cgcgcgacgg caccgaccgg
     2221 ctctcgcccc aggaactcgt ggcgactgcg gtgctcctgc tcaacgccgg gcacgaggcg
     2281 agcgtcaacg gcttcgggaa cgggttggcg tcgttgctgg cggcgggcgg tgacttcggt
     2341 gacccggtca ggctggtcga ggagatgctg cgacacgact cgccgctgca tcttttcgag
     2401 cgcacggcga cccacgacac ggtgatcgag ggggtccaca tccgggcggg tgagaaggtc
     2461 gcggccctgc tcggcgccgc gaatcgcgat cctgccgtct tcgccgagcc ggacgcattc
     2521 cggcccgacc gggatcccaa cccgcacttg gccttcggcg cgggcattca cttctgtgtg
     2581 ggagcgccct tggctcgact ggagttggcg acggctgctg gggtcctgct cggacggtgg
     2641 ccgaggctgc gcgtggtcga cgcggtgcga cgcccgacgt tcgtgctgcg cggctacgaa
     2701 cgcttggtca tcgcgccggc ctgacgcacg accacccaac ggaaggcacc gtgaccagcc
     2761 ggatcggtga gcgtcgccgc cgaggtcgcc cgaagcaacc gacgtcggta tgccgtctcg
     2821 tcacatcgcg ccaaggccgg gtcaggcatc ggcgcccaca ccccgaacga ctcgaacgcc
     2881 gttgtctgag tgtggatctc gtcatggtga agggagtcga cggcgacctg cgcagagagg
     2941 tcgcaactgc cgtccggcac gggtcgcacg gccgcgtccc cccggtatgc cgcgagcgtc
     3001 ccgttccggg gccgcgccgc cgcgacgtga ccggagtcga tcgcgatggc ggcgccgcgg
     3061 catacccgag agagcaggtc agcccacgcg aggtcacggg agagaccgac ctcgacccgg
     3121 tcaccctccg ccagcaacgg ccaatgccgt tgctgccagg cgagttccgc gtcggccagc
     3181 ggatcgccga ggtcctcgtc accgctggtc gggtcgacga gcacctcgcg gacgacaccg
     3241 tcagcgtcga gttggccgat gacacacggg acgacgtcga gccattcgtg ggccacgacg
     3301 aggacatcgg tgaggtcact caactcgtcg ggcagcggcg cgccgccagg cccacgcaac
     3361 cagcccaccc cgtcgggcag gtcggccggg cgttcgacga catcgacccc gagcagggga
     3421 cggtccggcc gatgggagtg caacgccccg atgagctcac cacggccagc accgatgtca
     3481 acgacgccgg agagctcgag caggtcggcg tagtgccaga tcgcctccgc gaggacggcg
     3541 cccagaggtg gatgggtcgc ggtggcgaag tctcgcgccg gcacctgccc gcgatagaac
     3601 ccgcgagggc cgtagagcgc ctcttgccat gcgtggaccc agccgatctt gcccatagtc
     3661 ggaaacctac tcgcgcacgc ccccactcca gaggagggta ggttcggagg atgagcacgc
     3721 catcggggcc gacggcccgg cttcgggtcg gggtcattgg cgccgggcgc gccggcgtcg
     3781 ccctcggagc agccctcgcc cgcgccgggc acctacccgt ggctgtcgcc gcggtcagcg
     3841 acgcctcccg cgagcgggcc gaggcggttc tgccgggcgt gcccatccgt gaggcgctcg
     3901 acgtggttgc cgaggctgat ctggtgctct tggccgtccc cgacgacgtg ctgcccgggt
     3961 tggttgccgg gctggccgcg gctggtgcct ttcaccccgg acaggtgctc ctgcacacga
     4021 gcgctcggta tgggatcggc gtgctcgagc ccgccgcgac ccacgacgtc ctgccggtgg
     4081 ccatccaccc ggcggtcgcc ctcaccggtg agcttggtga cggcgaacgc ctcgccgccg
     4141 cgttgttcgc ggtgaccacg ctcaagtcgc tgcggcccct cggggaggcg ctcgtcatgg
     4201 agatgggcgg cgagcccgtc tgggtcgagg aagaggaccg accggcgtat gccgccgcgc
     4261 tgggtgccgt ccgcgacggt cttgaggggg tgctcggggc ggcctcccgc gtgctggccg
     4321 actgcggcat acctcaccct gcccgtgcgc tcgcgccgct cgtcctcacc gcgctcgagg
     4381 acgcgctgcg gaccggtagc gttgcccgcg atccgggggt gtatgccgcg ccggtggtgg
     4441 atcggcccag cgacgggcca gagcccttgg aggacgatca gtgacgccga tccacatccc
     4501 gcgctggctg cgggcgccct ctccggggtg gtcgacgtcg gccgacgtca tcgtcgtcgg
     4561 gtcggggatc gcgggtctca ccgcggcact gcgactgcgc gcacgtggtg ccacggtgct
     4621 gctggtcacc aagaccctgc tcaccgaggg gtccaccgcg tgggcccagg gcggtatcgc
     4681 ggccgcgctg gccgacgacg actccccagg cgaccacctg catgacacgc tcgtcgcagg
     4741 cgtggggctg tgcgacatcc ccgctgtgac agctctggtc cacgaagggc cgacgcgggt
     4801 ccgcgaattg gtcactctcg gtgcggagtt cgaccggggg agcgacgggg cgatcagcct
     4861 gacccgggaa ggcggccacc atcgtgaccg catcctgcac gccggtggcg atgcgaccgg
     4921 caaggagatc tcgcgggccc tcatcgaggc cctgcaccgc gtcacggatg acccgggcat
     4981 cgagatcatc gagcacgccc tcgtcgtcga cctgctccag gacgccggag ggcgggtgtg
     5041 cggggcgacg atccacgtca tcggcgaggg gcagatcgac ggggtcggtg ccgcgcaggc
     5101 ccgcgccgtc gtcctcgcga cgggcggcct cggccagatc tactcctcga cgaccaaccc
     5161 gccggtggcg accggtgacg gcatggcgat cgctttgcgg gccggggcgg tcatggccga
     5221 catggagttc gtccagttcc accccacggt gctcttcctc ggggacgact cgaccggcca
     5281 gcagccgctc atctccgagg cggttcgcgg cgagggcgcc ttcctcgtcg atgtcgacgg
     5341 ggtgcgtttc atgcagggcg tgcacccgat ggccgacctt gccccgcgcg atgtcgtgtc
     5401 ccgggcgatc gtgcggcaga tggctgcgac gggctccgac cacgtcttcc tcgatgcgcg
     5461 tcacctgggc gccgagttcc tcgagcggcg cttcccgtcg atcgttgcgt cctgccgcga
     5521 gcacggcttc gatccggcga cgacgctgtt gccggtggct ccggcgcagc actatgccag
     5581 cggcgggatc cgcaccgacc tagtcggtcg gtcgtcgctc gacgggctct atgcctgcgg
     5641 cgagtgctcc tgcaccggtg ttcacggtgc caaccgcctc gcgtccaact cgctcctgga
     5701 gggcctggtg ttcgcccacc ggatcgccga ggacatcgcc gagcgcttcg cccagggcga
     5761 gttgccgtcg gtcacggcga cggccgccgc cccgcagtcc gacggcccgg acggttcagg
     5821 catcggcgag ggccggatgc gcgacaccgt gctgctgcgc tcggtccacc gcaaggccat
     5881 ccaacgcgcg atgaccagcg gttccggggt ggtgcgctcg gcctcgacgc tggagagcac
     5941 cgcttcggcc ctggcgacgc tggctgcgga ggccgatggc gacgaaggcg gccccaagtc
     6001 gtgggagacc accaacttgt tgcacctggg ccaactcctc acccaggtcg ccgccctgcg
     6061 tgaggagaca cgtgggggcc atgtccgcag cgacttcgtc gaccgcgatg acgcgcactt
     6121 cctcggccac gtccgcgcgg tccgggcggc ggacggcata ctcgcgacct ccttccaccc
     6181 caccgcgcct caggatcttt cgtgacccca gacctcgcct tcccggctgc cgatgcccgt
     6241 gccctcgtgc gcctcgccct cgatgaggac ctcggtccgc gccacgtcga tgtgacgacg
     6301 ttgtcgacga ttcccgccga tcaggagcgc acggcccacg tcgtggcccg cgcggccgga
     6361 gtgatcgcag gcgtgccggt cgtcgccgag gtgttcgggg cggtcgctga cctcatcggg
     6421 tgcgccaccc cggaggtcga gttgctcgtc gccgacggga ccgcggtggt gcgcggggac
     6481 gtcgtggctc gggtgtccgg cccggtccga gccatcctca tcggagagcg cacgatgctc
     6541 aacatcgtct ctcggctctc gggggtcgcc acccacaccc ggcgctgggc cgatcggctc
     6601 gatggcacgg gggcgatggt cctcgacacg cgcaagacga cgccggggat gcgtcacctg
     6661 gagaagtacg ccgtgcgcgc tggcggaggc accaacaagc ggatgggcct ctacgacgtc
     6721 gcgatgatca aggacaacca caagatcgcg gcgggtgggc tcgcgccggc gtatgccgcg
     6781 gtgcgccgca ccttccccga ggtcgacatc caggtcgagg tcaccaccgt cgcggaggct
     6841 ctcgaggcgt atgccgcggg cgcccgattc atcatgtgcg acaacatgtc ggtcgcgctg
     6901 ttgggtgaga cggtggcggc cctgcgggcg caggatgagc cggtggagat cgaggcgacc
     6961 ggcgggctga cgctggagat cgcccgggac tacgccctga ccggggtcga ctatctgtcg
     7021 gtcggcgggc tgacccactc gtcgccgatc ctcgacgtgg ccctcgacct cgtcgacnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnacg atctgagcga ccggggtcag
     7201 tcgggcagaa cgtgacggag gaactggctg ggtgctgcga ggaaccggcg gtagtggtcg
     7261 acgacggtca actcgtccca ggtcaccggt cgaaacccct ccgcgtcgag ttcgaggatc
     7321 tgggcgccgg gcagggcggc aatgatcggt gagtgggtgg caacgagcac ctgggcgccc
     7381 cgccgacgag ccagttgggc cagtcgaccg acgagggtga gttgtgcctc gaacgcgagg
     7441 cccgcctcgg gctcgtccat gacgaagaag ccttcgccgc ggaaccggct ggtctgcatg
     7501 agggcgagga aggactcccc atggctgaac cggtggaacg tcggatcgcg ggtgtaggag
     7561 tcgaccgccg tcgcatcgag atagcccatc agcgcgtgca tcgtctcggc gcgaacgaag
     7621 tatccccacc ggctgccgcc tggccctcgg acgatctgca gcgcctcgtg caggggtgac
     7681 tcgctgtcgt agctgcgatg ccgagcgccg gtcgatccgc cttcgggggc cagcccgaag
     7741 gccatcgcga tcccttcgat cagcgttgac ttcccgctgc cgttggcccc gacgagcacc
     7801 gtcgctgggc cgaggtcgag cccctcgcgc agcagttggc tgatgggcgc gagggttgcc
     7861 ggccagatgt cggtcggcag gtcactcccg gcacgaagct cgacccgccg gacgggcagg
     7921 ggtgggaacg gatcggcggc ggacccgcgg cggtgagcgg gcggcatacc tgagcgcttg
     7981 acaaccacgc gctcacggta tgccgccctg ccgacaggcc ctggtatgcg gcggtcaggc
     8041 tgtggtcacg cggcccgata agtcgcccac tgggcctgca tccgcgcgcc ttggcccggc
     8101 gtgaactgga acatgcaggc atcgtcggtg tagtccatga agttcgtgat ggggtcgagc
     8161 cccggcttgg tgcggcaact gtcacgcccg gtggggcagc cgaatgcggc ggacttctcc
     8221 gcagcggtat cggccacgta gtcgcccttg ccattacatc caccctggaa cgtgtggtag
     8281 agcccgagcc agtgcccgac ttcgtgggtc gccgtgtcgc cgaggttgta cggcgcggcc
     8341 gttccaccgg ggagggattc gtcgagcacg acgactccgt ccatgacatc gagcgttgct
     8401 ttggggaagg tcgcccagcc gagcaggctg tttccgaggt cggcggtgta gatgttgagg
     8461 gcagccttac cgccctgacg cagggccgtc ttcatgttcc gctcggcagt cgagccgttg
     8521 gtgagcccgt tgtaccaggc ggcattgttc gtcgagtcgg tgctgaccag cgtgaacgag
     8581 aagccggacc cggcataggc cgcgttgagg accgagatct ggttgctgat ctggttcgat
     8641 cccatgacgc cattggtccc atcggtgatc tggtgccaat agacgttgat cgggactgtc
     8701 gccacggcgg ccgtggcttg cgggagcatg gatcgggaag acttcgccag caccctggcc
     8761 tggaaatcgc tctccctcgc ggccacctcc gcgtcggaga gttcctgcgg atccttccgg
     8821 ttccccgggg caccgctacg agcgtcgttg accgaatccg tgtgctcgac acaggcctgg
     8881 gccgcggcgc cgctggcggc cgacgccggt gacacggcag cggccgaagc caccgacgcg
     8941 gccggaactc cgagggcgag gagtgtcacc gcgccgaggg caggaagggt caggagggct
     9001 ttacgcaaga tcatcgacgg accgtccttc gattggggag agggctcatc cggcgggttg
     9061 gacccgggcg cgcccaggtg gggagtgagg agaccgtatg ccgcccgccc accgcgcagg
     9121 ccctccgaat ggtcagaact cggtcaagaa atagtgtgca cccgcaaccg cccaatgcga
     9181 agggcctttg ccgagaaccc gtttcgaggc gccgaggggc gtcggggagg catcgtggga
     9241 ctcggtaccc tggaccggtg accagcgtcc ccaccgacca gaccgtgccg accgatccca
     9301 tcggcccgag cgacgaggtc gacgacctcc cagaacagct ccgggtccgc cgggacaagc
     9361 gcgaccggct catcgccagt gggcacgcgg catacccggt gtgggtggac cggactcaca
     9421 gcctcggcga cctgcgccgg gactgggggc accttgaggc cggacaggag accgatgccg
     9481 tggtccgcac cgcgggccgg gtgatgtaca tccgcaacac gggcaagctc tgcttcgcga
     9541 ccctccagga gggggccggg acgcggctgc aggtcatgct cagcctcgcc gaagtgggtg
     9601 aggcggccct cgccgactgg aaggccctgg tcgacctcgg cgatcacgtc gccgtcgaag
     9661 ggcgggtcgt cagcagcaag cgcggtgagt tgtcggtcat ggcgacgtcc tggcagatgg
     9721 cctccaaggc gctgcgcccg atgccgacgc tgcacaagga cctctccgag gagacgcggg
     9781 tgcgccagcg ctacgccgac ctcgtcgtgc gccaggaggc ccgcgacatg gtccggttgc
     9841 gggcgagagc cacccgcgcg atccgcgagg tgctcgacac gcagggctac ctcgagatcg
     9901 agacgcccgt cctgcaactc gtccacggcg gcgccgcggc gcgccctttc cgcacgcatc
     9961 tcaacgcctt cgaccagcag atgaccctgc gcatcgccct tgaactcaac ctcaagaagg
    10021 cggttgtcgg cggcgtcgac cgggtctacg agatgggccg catcttccgc aacgagggcg
    10081 tcgacggcac ccattcgccc gagttcacca tgttggaggc ctatcaggcc tacggcgacc
    10141 agacgacgat cgcggcgctc atccgtgacc tctacctcgg cgtggccgac gccctcggca
    10201 cgcgggtcgt gcagacgccc cgtggcgagg tcgacctcgg cggcgagtgg cgctggcttc
    10261 cggtttacga cgctgtcagc gacgccgtcg gcgaggaggt caccctcgcc acgcccgtgg
    10321 agacgctgcg ccggtatgcc gccgcgcacg acgtcccgct cgaccccgcc tggggggcgg
    10381 acaaggtcgt cctggagttg ctgtccgagt tggtcgagcc gaaggtcctc cagccgacct
    10441 tcttctgcga ctatccggcg atcgcccagc cgttggcgcg cccccaccgc tcagtgccgg
    10501 ggctcatcga ggcctgggac ctcatcatcg cgggggtcga gcgaggcacc ggattctccg
    10561 agctcatcga cccggtgatc cagcgccagg tcctcgaagc tcaatcgctg ctcgccgccg
    10621 ggggagaccc cgaggccatg gcgctcgatg aggacttcct gcgggctttg gagttcggtg
    10681 cccccccgat gggcggtcta gggctcggaa tcgaccggct cgtcatgctc ttcactggcg
    10741 cctcgatccg cgaaaccatc ctcttcccgc tcttgaagcc ggaggactga ccatgtggga
    10801 cgcgatctac ccctatctcg ccgccatcct gccgacgatc ggcctgctgg tgatcgccta
    10861 tgtcgtcttc aagacgatct tcgaggccga ccggcgcgaa cgaaaagccg ttgcggaatg
    10921 ggaacgcagc cacgacgcgt cgccgatcag ttctaccgac gacgagtcgc gaccgacgac
    10981 ttagtcgtct gcggccgacg ccgcgacctt tcggacaatt ctgcccggga ggcttgccga
    11041 cgcatcgagg tcacgaccca ttcaccgaga tcaaagggga cgggccagga tcaccggagg
    11101 cgatgaaccg gtgaccgacg cgcaacgacc atcgggcgcc acggaccccc ttcctggagt
    11161 ttgtagattt ccgcgtgtta cgatcacccg cggtgatcga cgaagtttcg cgggattctt
    11221 cgccaaccgc caggtaagac ggcacggagg gttagccttg acccacggtc cgtagagtga
    11281 cattcaacaa agccgggcaa ccggcgtttt cctccgcagc agttccctca tgaatggagc
    11341 acccaccgtg gccaagcgtg ttcaggtcgt cctcgaagac gacgttgacg gcagccccgc
    11401 agacgagacc gtgtctttcg gtctcgacgg cgtttcttac gagatcgacc tttcggccga
    11461 gaatgcctcg gccttgcgtg accagctcgc caaatggatc ggtgccgccc agcgcaccgg
    11521 tggtcggcgg gccgcagggc gtcgcgccgg gggctcggcg cgacggactg acgtttcagc
    11581 cgttcgggcg tgggcacggg ccaacggcca ccagatcaac gagcgtggcc gggtgccggc
    11641 ggcggtccag gcggcctacg acaaggcaca tgcctgatcg agcggttccg cacggtccgg
    11701 acgggtcgca ctccttgggg agggcggccc gtccgctgtt tcactggggc aggaccgccg
    11761 cggcaagatc tccgaggcgc tcgacccgat cgagcacctc agtggccgtg acctgagcca
    11821 gggccccggg agtcgccgcg ccctcctgca cctcgtccca cgtgcgcgga gcagcgacga
    11881 acggtgccgg tcggccgcgc aatgaatagg gcgagatcgt cgtcttggcc gcgacattct
    11941 ggctccagtc gaagaacacc ttgcccggtc gcaacgaggt cgtcatcttc cagacgacga
    12001 ggtcgggatg ggcggccgtc aattcctggg ccaacgtccg ggcgaccagc gcaatgtccg
    12061 ccgacgcgtg ggagcccggg agggcggcat acaactgcat gcccttggac ccgctggtga
    12121 cggggatcgg gtccagcccc agggcccgaa gccgctccct tgccagcagg gcgacgattg
    12181 agcactcctg tagccctgct ccggggccgg gatcgaggtc gacgacgagc cgatcgggaa
    12241 aggccggcag cccgtccgcg ccgatctgcc attgcggcac gtgaaattcc agcgaggcga
    12301 ggttggccag gtaggtgagc gcgtccagcc ccgtcaccat cggataggtg atgggctcgc
    12361 tggtgtgcgt gagggtgacc cggggcagcc atgccggcgc ccccgacgga aggttcttct
    12421 cgaagaagtt gaggccctcg accccctcgg ggaaccggac ccgagtgact ggacggcccg
    12481 cgagatgtgg cagcagcacc ggcgcgatcc gcgcgtaata gtgcaacacc tcccccttgg
    12541 tcgtctcggt cgccgggtag atgactttgt cgaggttcga cacgaccagt cgccgtccac
    12601 cggcatcgac ggtcaccttt gcggggtcgg tcggctcagg catcgggcaa ctcggtcggg
    12661 tcgatgtcac cgggcgtgag gtccggacgc aatcctcggt atgccggttg gcgcagtcgg
    12721 cggctgtcgg tgagtcccag cgcttggacg tcgcagacaa gcaccgggtg gacccaggtg
    12781 gtgcctgagg cgtcctctgg gggcacctcg tcgacgaacg ggctcccggc ggaggtcagg
    12841 ggcgccaggt cggcggcgag cgccctggcg gtcttgccgg cgatccccga cccgacccgc
    12901 cccaggtaac gcagccctcc aacccccggc acgccgacga ggacggcccc gagccggcct
    12961 acctggtcgg tctccggtcg ccagcctccg atgacgaccg attgcgaggt gcggtgcggc
    13021 agtttgaccc agtcctcggt gcgggcgccg gcggcatacc gaccggatcg gcgcttgctg
    13081 acgatgcctt ccaggccctg ctcccgggtg gcctcccaca gcgcctgccc gtcggtgaag
    13141 gtgggcggca ccgtccagtg cgggttctga agttccagcc gctcgagggt tgcccggcga
    13201 tcggtccacg accggccggt gaggtcgacc ccgtagaggc gcaagaggtc gaagaccatc
    13261 agggtcaccg gcgcgcgggc ggccagggcc gcagccttcg ttgggctggt cacgtggaat
    13321 cgctcggcca gagccgcaaa cgagggtatg ccgtcgcgca gcaccaccgc ttcgccgtcg
    13381 agcagcatgt cctcgaattc gtttgccaga cctgctagtt cggggaaggc gacggtgaca
    13441 tcccgttcgg tccgagacgt gaggcgcacc tggccgtcgt ggatgtcggc gaggatccgc
    13501 atcccgtccc acttgacctc atgggcccac tcgggacccg tcggcactcg acgggtgggc
    13561 gtggcgagca tcgggcgcat gcggctcaga ggatggcgac gagaacggcg gagctggccc
    13621 gcgccgcccg ccgatagaac ttgcgcaagt ccttgtaacg cggtcgcaac cacgtgtgga
    13681 acacgtcctg ctcgtcccag aacccggggt agatatcggc gccccgcatc gcctcgaaat
    13741 cgactcgggc ggcgacctcc gaggctggga gggcctcgag cgccgcactg atc
//
LOCUS       DAOMZY010000041        13754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_4085_length_13754_cov_5.795387, whole genome shotgun sequence.
ACCESSION   DAOMZY010000041 DAOMZY010000000
VERSION     DAOMZY010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 13754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13754
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4085_length_13754_cov_5.795387"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="PLK64_03900"
     CDS             complement(<1..177)
                     /locus_tag="PLK64_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HPV78914.1"
                     /translation="MFFSNALIGLREGLEAALVVVILIAFLVKTDRRWALRYVWIGVG
                     AAVALSVVIGAVLTF"
     gene            complement(282..1199)
                     /gene="pheA"
                     /locus_tag="PLK64_03905"
     CDS             complement(282..1199)
                     /gene="pheA"
                     /locus_tag="PLK64_03905"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HPV78915.1"
                     /translation="MTRYGFLGPEGTFCEMALRQWAASHEGDHHPFGSVGAALRALRE
                     GRIDAAMVPIENSVEGGVSATLDALSVGEQLHIIGEVLVPITFVLAARPGMPLSRVRA
                     VGTHSHAWAQVRGWMDANLPDAAYLPTLSTAASAQTLATETDPPFEAAVCAPGAATRF
                     GLDVLAEDIGDNGGAVTRFVLVSRPGHLPPASGADKTTVVLFQKLDRPGGLLELLEQF
                     AVRGVNMVRLESRPTGEAMGSYCFSIDFEGHLAEERVRESLIGLKRVCADVRFLGSYP
                     RADGRQGIIDAGMGDDDFVVARAWIDALS"
     gene            1275..1910
                     /locus_tag="PLK64_03910"
     CDS             1275..1910
                     /locus_tag="PLK64_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HPV78916.1"
                     /translation="MTFSIAIAAAAFALIFPVEFPDKTFVATLVLATRFRPLLVWIGV
                     ASAFLVQTVVAVLAGQLLANLPRTPVEIASGLLFLIGGVILLRGAAKADDEEAEAEAE
                     FTEVAASKAGATGVKAVATSFLVLFLAEWGDLSQLLTANLVVRYGEPLSVGVGAFLAL
                     ATVSGLAAALGHQLLRRVKLATIRRIGGGVCLALAALSVLGLFGVDVPILG"
     assembly_gap    1913..2012
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2065..2964)
                     /locus_tag="PLK64_03915"
     CDS             complement(2065..2964)
                     /locus_tag="PLK64_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5926 family protein"
                     /protein_id="HPV78917.1"
                     /translation="MGKASKRRETRHTSGSDRPAAAPFVRRPFEGLPSETEWVAMREI
                     LPAATAAVRLVDGPATAGLPSDAPREATLATVLPMAWPGLHRTGGGVLVATQAGATCG
                     DASRDIAAAWLLATANPEGTPLTQVPTPTADTPRLQDLLDPKALFEVTLHEGFGFWVG
                     DTELDEDGAASLEQANASIVPSKRIAGAPTVFWVRFGERTFVRWVLPQDESTATDGLA
                     RLTAADANRLGGDARLLGAFRACGLLVPVWEVEPEAEADSFVDAVTAMAGPLADAIAD
                     TTPLTAEERRARSGLLSRQVTLR"
     gene            complement(3045..3992)
                     /locus_tag="PLK64_03920"
     CDS             complement(3045..3992)
                     /locus_tag="PLK64_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPV78918.1"
                     /translation="MTLWGTAGCTLVSSWSQNASAPSATSTSLPLRPASRPLAQASTP
                     GQKTTVAVISDFGQCDDGEQQVADMVRSWDPALIFSAGDNSQGVEGCVPFIESVGAYY
                     GDYVDGPDGPRLYPALGNHDYEDEGAGLAAYRAYFDYLETDDDFLGRWHLKSIGDLNV
                     YLLDSNAPADDMPLQQHFLRSALGQAAPNTWNVVIFHHPPFTSGPHDPRLDMRPEAGW
                     NFKEWGVDLVINGHQHVLEDVVVDGLHYVTVGVGGQTLVRPCPPVRALGSLTCMEGVG
                     AARLVTTDQALSLEYRQPGADPGSAYRLEIPRSSLTRGS"
     gene            complement(4217..>5638)
                     /locus_tag="PLK64_03925"
     CDS             complement(4217..>5638)
                     /locus_tag="PLK64_03925"
                     /inference="COORDINATES: protein motif:HMM:NF019176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78919.1"
                     /translation="LGTYDHTESLGAMTNAEYIKNADLLRLRGNGWYRQLFRNPAFVT
                     AANTLWQTGGAGYAATQLPAKIAQYKTQIEGSAANNFAKWRVLGTPTHLVAGEGRPYS
                     STYAGEVSYLSTWVTARVNHLRKAYGDIPLLRYSGHASTLGWLPTVDIGQIGGTVNQA
                     LTLESVSYFIENSTVAGSLESNAHVASIGWMGWRGSPMGTTGRGLPMEALQLRLTGDL
                     ATKYDVSYRAHVRNVGWQPWVTNGATAGTTGQAKPIEAVMIRLLAKNPAPPTGGVSPT
                     ATPTATATATATATATATATATSPSPTATATATTTSPTPTATATATATATATTTTPPT
                     ISASPAYSAHVATIGWMGTVGSDVVAGTTGRGLAMEALRLQIQASGVTGGISYRGHVS
                     SIGWQPWVTSSTYIGTTGRALKLEAFEIKLTGDLATKFRVEYRAHVSGIGWQSYVADG
                     ATAGTTGQARSVEAVQIRLVPKV"
     assembly_gap    5639..5738
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5739..6073)
                     /locus_tag="PLK64_03930"
     CDS             complement(<5739..6073)
                     /locus_tag="PLK64_03930"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPV78920.1"
                     /translation="MSITLNDLDPTHNTLAYVHGDKDRVVPSLVGIEDGAGTYNLAAS
                     AAEFKGRGNYTWGLPKKPYQIKFNSSTAILGMPKAKAWVLLANDADGSLMRNKLAFDY
                     ALAIGSPYAP"
     gene            complement(6501..6887)
                     /locus_tag="PLK64_03935"
     CDS             complement(6501..6887)
                     /locus_tag="PLK64_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPV78921.1"
                     /translation="MPWTAQGAPQVRRALVEDLTALGVSGEVIDEAEIVVAELVSNSL
                     RHARPLSDGCLRVRWKTKGGVVEVEVADGGGPSTPRPAPPALWSSSGRGLRIVRSLAH
                     EWGVHEEKHGRTVWASLGGPSRRRTH"
     gene            complement(6992..7798)
                     /locus_tag="PLK64_03940"
     CDS             complement(6992..7798)
                     /locus_tag="PLK64_03940"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPV78922.1"
                     /translation="MPQGGDVKERRPAVIAHRGASAIRPEHTLGAFRQAIRDGADAIE
                     CDVRMTLDGHLVCLHDRSVDRTSDGHGLVSRHTLRQLQALDWSGPGGESRHTATADDR
                     RVVTLAELAELVRTAGRPLGLVVETKHPSRWGPHVDAEVVRVLGEAGLGGPSEGRVPS
                     AVVLSFWPVSLRRIGLHQPQLARGQLFAAAAPGTARGSLAPGVSIAALDIALVRRRPH
                     LVARHQARGHRVYAWTVNDPADLRHCQDLGVDAVIVDDPGAALAVLDGRR"
     gene            7971..8453
                     /locus_tag="PLK64_03945"
     CDS             7971..8453
                     /locus_tag="PLK64_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPV78923.1"
                     /translation="MTLPPFQLLVDAHWRDVARLAHALVGPDDGDDVAQQAWLQAWAA
                     YPSLTSATNLRGWLFTVTYRCATDAHRGRGRRPVPVEDPALAAAERPAVDPPATASDE
                     SGVWQIVATLPPRQREAVALRYVADLDHAAIAAALGTSTAMSRRLVSDALGSLRKALS
                     "
     gene            8450..9058
                     /locus_tag="PLK64_03950"
     CDS             8450..9058
                     /locus_tag="PLK64_03950"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPV78924.1"
                     /translation="MTPSHSGPSDRLEASLAGFDVPAARPPDFPTADVSYAVDDTPIG
                     RMLLACDGSGVLVASLFTPDAAAEEAALQRLAARISPRVLRRPQSLDEARRWLESYLD
                     GGRPGAAPAWALSLATPFQIPVLTTLAARVGYGDRATYGQLASWAGRPQAARAVGTAL
                     GGNPLCVVLPCHRVVGASGSLTGYAGGLAAKRYLLELESTSL"
     gene            9209..9796
                     /locus_tag="PLK64_03955"
     CDS             9209..9796
                     /locus_tag="PLK64_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78925.1"
                     /translation="MTARTSPTPAPTPLDDDRVTELVTRAAYERLRSDLAVAHGTELV
                     SWDEQNPKMRGDLRESVEPTVQDTIEAIAELGFAVVPYEWLGGQASDATAVVIGEATL
                     SPAATTPNEVDIDLPASPQRLLTLASMTSRLTSSTNATAPGEQVADESEPVDPSALRG
                     LVSGAGSAAVAIVRLPQTALRPIKRVVARVMGPAA"
     gene            complement(9827..10780)
                     /locus_tag="PLK64_03960"
     CDS             complement(9827..10780)
                     /locus_tag="PLK64_03960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV78926.1"
                     /translation="MTDDVTPANDQTATAGAASLSLPDGLTSRPLGPSDVRAVFEVMA
                     AVELADAGKVAIEEADIAADWQRPSFDIAAKTIGVFDGDRMVGYGEHSGGDRSDAAVH
                     PAYRGRGVGTAVARWIQATARAAGVSVVGMPVPAGSPSEALLRALGYHQRWTSWILEV
                     PQGREIASQPLPQGFSLRIAETAADHRAAWEVIEDAFLEWSERPRTPYEDWAAVTMER
                     PGFEPWNLRLAIDPSGAVVGVSLVLLADEVGYVDKLAVRKDVRNQGLARALLVDSFAA
                     ARAHGATVCELSTDSRTGALGLYEKVGMEVTSIWFNLAIDL"
     gene            complement(10915..13491)
                     /locus_tag="PLK64_03965"
     CDS             complement(10915..13491)
                     /locus_tag="PLK64_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0051731 - polynucleotide
                     5'-hydroxyl-kinase activity [Evidence IEA];
                     GO_process: GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide kinase-phosphatase"
                     /protein_id="HPV78927.1"
                     /translation="MSELHIPALSLVVLIGASGSGKSTFAARHFAPFEVVSSDFCRGL
                     VSDDVNDQSVSGDAFEVLDFIVGKRLSRGRLSVVDATNVQREARRQLVELAKRHDVLP
                     VALVLDVPESVALARNRTRPDRDFGEHVVRRQREQLRRSIGGLAREGFRVVHVMRGVE
                     EIEAATVVRDRLFNDLRDEHGPFDVIGDVHGCLSELLSLLDALGYVVERDAQGRPIDA
                     AHEAGRQVVFVGDLVDRGPDSPGVLRLVMGMAAAGHAFAVPGNHENKLVRALSGRKVR
                     VSHGLETTLAQLDVEADEFRESVRRWCDGLVSHLVLDDGRLVVAHAGLPERYHGRASG
                     RVRAFALYGDTTGETDDFGLPVRYPWALDYRGRATVLYGHTPTVTPEWVNGTMCLDTG
                     CVFGGHLTALRWPEREVVQVPAERVWYEPSRPFPTADPDSLSGTQARVDPTRGREDLD
                     LTDVLGRRVIETSHHGRISVRDDAAAGALEVMSRFAMHPRWLAYLPPTMSPVETTARE
                     GLLEHPDEAFAAYVQWGVGDVVCEEKHMGSRVVVLVCREPAVAARRFGADRGEIGVAY
                     SRTGRSIFGPEQSAAWLTSLANAVTKAGLWDELSTDWLLLDAEVLPWSVKAQPLIREQ
                     YAAVGAAARGSLSAASASLRAAAARGLDVGDLLARNELRRANADAFAQAYRRYCWEVD
                     GLEGVSLAPFQILASEGATWESRDHLWHLEIADKLVEADAALFRSTRRLVVNVGDAVS
                     RAEGVAWWEELTAAGGEGMVVKPLANLTRNTKGLVQPGLKVRGREYLRIIYGPDYTMP
                     EHLTRLRDRKLGHKRSLALREYALGLESLGRLVQGEPLWRVHEAVFAVLAMESEPVDP
                     RL"
     gene            complement(13488..>13754)
                     /locus_tag="PLK64_03970"
     CDS             complement(13488..>13754)
                     /locus_tag="PLK64_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3' terminal RNA ribose 2'-O-methyltransferase
                     Hen1"
                     /protein_id="HPV78928.1"
                     /translation="VIVTTPNSEFNVRYPNLAAGALRHPDHRFEWTRAEFGAWASRVA
                     TAYGYAVSFRPVGDVDPDLGPSTQLALFTRGDTVARNRAAEVTG"
BASE COUNT         2222 a         4742 c         4513 g         2077 t
ORIGIN      
        1 gaaggtgagg acggcgccga tcaccaccga gagcgcgacc gcggcgccca cgccgatcca
       61 gacgtaccgc agcgcccacc gacggtcggt cttgacgagg aaggcgatga ggatgacgac
      121 gacgagcgcc gcttccagac cctcgcgtag gccgatgagg gcgttggaga agaacacggc
      181 actccttagg aggaaagtta ggtaaccctt acctgttcag gatggcacag gtaagggtta
      241 cctaacaact gcggtcctgg gcggcatccc gagcgttgga gtcacgagag ggcgtcgatc
      301 caggcgcggg cgaccacgaa gtcgtcgtca cccatccccg cgtcgatgat cccctggcgg
      361 ccgtcggcgc gcgggtagga accgaggaat cgcacgtcgg cgcacacccg cttgaggccg
      421 atgagcgact cgcggacgcg ctcctcggcg aggtgcccct cgaagtcgat cgagaagcag
      481 taggaaccca tcgcctcgcc agtgggtcgc gactccaggc gcaccatgtt gacgccgcgg
      541 accgcgaact gctcaagcaa ctccagcagg ccacccggcc ggtcgagctt ctggaacagc
      601 acgaccgtcg tcttgtcggc cccgctcgca ggcggcaggt gccccggccg gctgacgagg
      661 acgaagcggg tcaccgcgcc accgttgtcg ccgatgtcct cggcgaggac gtccaggccg
      721 aagcgggtcg ccgctccggg ggcgcacacc gccgcctcga agggcgggtc ggtctcggtg
      781 gccagcgtct gcgccgaggc cgcggtcgac aaggtgggca ggtatgccgc gtccggcagg
      841 ttcgcgtcca tccagccgcg cacctgagcc caggcgtgcg agtgggtgcc gacggcgcga
      901 acccggctca acggcatacc cggtcgggcg gcgagcacga acgtgatggg gacgagcacc
      961 tcgccgatga tgtgcagttg ttcacccacc gacagggcgt cgagcgtcgc gctgacgccg
     1021 ccctcgaccg agttctcgat cggcaccatg gctgcgtcga tgcggccctc gcgcaaggcg
     1081 cgcagggcgg caccgaccga gccgaacgga tggtggtcac cctcgtgcga ggccgcccac
     1141 tgccgcaggg ccatctcgca gaaggtcccc tcaggcccga ggaagccgta gcgggtcatc
     1201 gggtccacgc ctcgggacgt cggttgggct ggcgagtggg cacgttcggc agggtattac
     1261 gctcgatgct cgttatgaca ttcagtatcg ccattgccgc tgccgcgttc gcgctcatct
     1321 tcccggtgga gttcccggac aagacgttcg tcgcgacgct cgtgctggcg acccgcttcc
     1381 gaccgctcct cgtgtggatc ggcgtggcca gcgcgttcct ggtgcaaacg gtggtcgccg
     1441 tgctcgccgg gcaactgctg gccaacctgc cgcggacgcc cgtggagatc gcgtcgggcc
     1501 tgctgttcct tatcggcggg gtcatcctgc tgcgcggggc ggcgaaggcc gacgacgagg
     1561 aggccgaggc ggaggccgag ttcaccgagg tcgccgcgag caaggccggg gccacggggg
     1621 tcaaggccgt cgcgacgagt tttctcgtgc tgttcctcgc cgaatggggc gacctgtcgc
     1681 agttgctgac ggccaacctc gtcgtccgtt acggcgagcc gttgtcggtc ggcgtcgggg
     1741 cgttcctcgc gctggcgacc gtgtcggggc tcgcggcggc gttgggacac cagttgctgc
     1801 gccgggtgaa gttggcgacg atccgccgca tcggtggcgg ggtctgcctg gcgctggccg
     1861 ccttgagcgt gctcggcctg ttcggggtgg atgtgccgat cctcggctga ggnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgcggctg cctcgagctg aggcgcgcgg
     2041 catacgtgaa gggcgggcct cgcctcagcg caaggtgacc tgacggctga gcagcccact
     2101 gcgagcgcga cgctcctcgg ccgtcagcgg cgtggtgtcg gcgatcgcgt cggccagcgg
     2161 gccggccatg gcggtcactg cgtcgacgaa cgagtcggct tcggcctcgg gctcgacctc
     2221 ccacacgggg acgagcagcc cgcacgcgcg gaaggcgcca agcaggcggg cgtcgccacc
     2281 cagccggttg gcgtctgctg cggtgagccg ggccaggccg tcggtcgccg tcgattcgtc
     2341 ctgcggcagc acccaccgaa cgaaggtccg ctcgccgaac cgcacccaga acacggtggg
     2401 ggcaccggca atccgcttcg acggcacgat cgaggcgttg gcctgctcca acgaggccgc
     2461 accgtcctcg tccaactcgg tgtcaccgac ccagaagccg aagccttcgt gcagggtcac
     2521 ctcgaacagc gccttcgggt cgagcaggtc ctgaagccga ggcgtgtccg ccgtcggggt
     2581 cggcacctgc gtgaggggcg tcccttccgg gttggcggtc gcgagcagcc aggctgcggc
     2641 gatgtcgcgg ctggcgtcgc cgcacgtcgc cccggcctgg gtcgccacga ggacgcctcc
     2701 gccggtgcgg tgcagccccg gccacgccat cgggagcacg gtggcgaggg tcgcctcgcg
     2761 gggagcgtca gacggcagac cggccgttgc cgggccgtca acgagtcgaa ccgccgccgt
     2821 cgccgccggc aggatctcgc gcatcgcgac ccactcggtc tccgagggca gcccctcgaa
     2881 gggcctgcgc acgaagggtg ccgccgcggg tcgatccgac ccagaggtat gccgtgtctc
     2941 gcgccgtttg ctcgccttac ccatagccga ccaccctagc cgggttccac cgggcccgtc
     3001 gcaagccgag gcggcgtcga gcaccagctg gcgcgggagg ccgttcagga ccctcgcgtg
     3061 aggctgctac ggggaatttc caggcgatag gcagacccag gatcggcgcc cggctgtcgg
     3121 tactccaggc taagcgcctg gtcggtcgtg acgaggcgtg cagcaccgac tccctccatg
     3181 cacgtcaggc tccccagagc ccggacgggc gggcagggcc ggacgagagt ctgcccccca
     3241 accccgacgg tgacgtaatg gaggccatcg accacgacgt cctcaaggac gtgttggtga
     3301 ccattaatca ccaggtcaac gccccactcc ttgaagttcc accctgcctc ggggcgcatg
     3361 tccaaccgag gatcatgtgg cccagaggtg aaaggcgggt ggtgaaagat gacgacgttc
     3421 cacgtgttcg gagcggcttg ccccagagcg gaccgaagga agtgctgctg gagtggcatg
     3481 tcgtcggcag gtgcgttgct gtcgaggagg tagacattga ggtcgccaat cgacttgagg
     3541 tgccacctgc ccaggaagtc gtcgtcggtt tcgaggtaat cgaagtacgc gcgatatgcc
     3601 gctagaccag ccccttcgtc ctcgtagtca tgattcccca gcgctgggta cagccgcggg
     3661 ccgtcaggtc cgtcgacgta gtctccgtaa tacgcgccga ccgactcgat gaagggcacg
     3721 cagccctcga caccttgaga gttgtcgccc gcgctgaaga tgagagccgg atcccacgag
     3781 cgcaccatgt ctgcgacctg ttgctcgccg tcatcgcatt ggccgaaatc agaaatcacc
     3841 gcgacagtgg tcttctggcc cggcgtactt gcctgagcca acgggcgact ggccggacgc
     3901 agcggcaacg atgtgctcgt ggcgctgggt gctgaggcat tctgcgacca cgagctgacg
     3961 agcgtgcacc cagcggtacc ccacagggtc atcaacacga gggccgcgga tgtcgagcga
     4021 cgtcgagtca tgggcaactc ctgtcaagtc ctcgtgaaag gaacatcaga tcatggcgcc
     4081 gactagagcg gaagccgaac gcgttgcgcc gccgcgggcg cgggagcccg tgccgttcca
     4141 ccagcccggt tcgagcacgc gcaggtgtcc ctgccggatg agtccggcag ggacacctgg
     4201 gtgatgcgcc taagggtcag accttcggca cgaggcggat ctgcactgcc tcaaccgatc
     4261 gggcttgccc tgtcgtgccg gcggtcgcgc catcggcaac atacgactgc caaccgatac
     4321 ccgacacgtg agctcgatat tcaacccgga acttcgtggc cagatcaccc gtcagtttga
     4381 tctcgaaggc ctccaacttg agcgccctac cggtggtccc gatgtaggtg gacgaggtca
     4441 cccacggctg ccagccgatg ctcgagacgt gaccacggta gctgatgcct ccggtaaccc
     4501 cggatgcctg gatctggaga cggagagctt ccatggccag gccccgtccg gtggtgccgg
     4561 ctacgacgtc actacccacg gtacccatcc acccgatcgt ggcgacgtgc gcgctgtagg
     4621 caggcgaggc agagatcgtt ggcggcgtcg tagtcgttgc cgtggctgtt gccgtggcgg
     4681 tagccgtcgc ggtcggtgtc ggtgacgtgg tcgtggccgt ggccgtggcc gtggggcttg
     4741 gggacgttgc cgttgccgtt gcggtcgcgg tcgcagtcgc agtcgccgtt gcagtcgcag
     4801 tcggcgtcgc agtcggcgag acgccaccgg tgggaggagc agggttcttc gccaggagcc
     4861 gaatcatcac tgcctcgatc ggtttggcct ggccagtcgt ccctgccgtg gcaccgttgg
     4921 tcacccacgg ttgccaaccg acgttccgga cgtgcgcccg ataagaaacg tcatacttcg
     4981 ttgccaggtc acccgtcagc ctcagctgga gggcctccat tggcaagcca cggcccgtgg
     5041 ttcccatggg ggacccacgc cagcccatcc agccgatgct ggccacgtgg gcattggact
     5101 ccagcgagcc ggcgaccgtc gagttctcga tgaaatagct gacggactca agggtgagtg
     5161 cctggttgac agttccgcca atttgcccga tgtcgacggt cggcagccag cccagcgtgg
     5221 acgcgtggcc gctgtaccgc agcagcggga tgtcaccgta ggccttacgc aagtggttca
     5281 cccgcgcggt gacccaggtg ctgagatagg agacctcgcc ggcgtaggtc gatgagtagg
     5341 gccgaccttc acccgcaacg aggtgcgtcg gggtgcccag gaccctccac ttggcgaagt
     5401 tgttcgccgc cgagccttcg atctgcgtct tgtactgagc gatcttcgct ggcagttgag
     5461 ttgcggcgta cccagcaccg cccgtttgcc aaagggtgtt ggccgccgtg acgaacgccg
     5521 ggttgcggaa cagctgccgg taccaaccgt tcccgcgcaa gcggagcagg tcggcgttct
     5581 tgatgtactc ggcgttggtc atggccccaa gggactcggt gtgatcgtac gtgcccagnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg agcgtaagga gaaccgatag
     5761 cgagggcata gtcgaacgcc agcttgttac gcatcaggga accgtcggca tcgttggcga
     5821 gaagcaccca cgccttggcc ttaggcattc cgagaatcgc cgtcgacgaa ttgaacttga
     5881 tctgataggg cttcttgggc aggccccatg tgtagttgcc gcggcccttg aactctgccg
     5941 ccgaggccgc gaggttgtac gtccctgccc cgtcctcaat ccccaccagg gagggaacga
     6001 cgcggtcctt gtcaccatgg acgtaggcca gcgtgttgtg cgtcgggtcc agatcgttca
     6061 gggtgatgct catcgtggga aggttcgccg taatcggcgc ggcctcggcg ggagtggccc
     6121 gcgtcccgat ggcaaagatc aatgaaagga ggacgaccca tgcccccatg gaagacgcgt
     6181 agacgcgtgc gggtcgagaa cgagccattg gatatccctt gcttcggtgt cttgggtcaa
     6241 cagcgacagg cagcaggcac cggcgcgcac cccgcggcgc gcaccagcca tggaagccca
     6301 ttggagaaga tagcgcgatt ctcgccatac gcaaaccagc ccggagagag tgaaaggtca
     6361 cgcttgcccc cgacaggccc agccgccagg agagagagcc ccggtgagcg gaagcgaata
     6421 tccaactggc agagcgcaac gatgaacaag tggtgaacaa gaagcgttca gggatggggg
     6481 ccagttaagc ggagtgacgc tcagtgagtg cggcggcggg acgggccacc gagcgaggcc
     6541 cacacagttc gtccgtgctt ctcctcgtgg acgccccatt cgtgggccag gctccgcacg
     6601 atccgcagcc cgcgaccgga gctcgaccac agcgccggcg gtgctgggcg cggcgtcgag
     6661 ggcccgccgc cgtcggccac ttcaacctcg acgacgccgc ccttggtctt ccaccgcacc
     6721 cgcaggcatc cgtcgctgag cggtcgggcg tggcgcagtg aattggaaac caactcggcc
     6781 accacgatct cggcttcgtc gatgacctca cccgagaccc cgagtgccgt gaggtcttcg
     6841 acaagggccc ggcggacctg gggagcgcct tgtgcggtcc acggcatacg gatggtgcgc
     6901 ggcgcctccg gatcggtgag tcgaattgct tcgtcagcca cagatgcgcc tttcacctac
     6961 gttcgccggt gcgatgccga cttaggccac cctatcttcg gccgtcgagg acggcgagcg
     7021 cggcccccgg atcgtcgacg atgacagcgt cgacacccag gtcctgacag tggcgcaggt
     7081 cggcggggtc gttgaccgtc cacgcataca cgcgatgccc ccgcgcctgg tgccgcgcca
     7141 cgaggtgcgg gcgccgccgc accaaggcaa tgtccagcgc cgcgatggag acccccgggg
     7201 ccagcgaacc gcgggcagtc cccggcgcag cagccgcgaa caactgcccc cgggcaagtt
     7261 ggggctgatg aagcccgatg cgccgcaacg acactggcca gaaggacaga acgaccgccg
     7321 aggggacgcg accctccgag ggacccccga gccccgcctc gccgagcacc cgcaccacct
     7381 ccgcatcgac atgtggcccc catcgactcg ggtgcttggt ctcgacgacg aggccgagcg
     7441 gacgccccgc cgtgcggacg agctcagcca actcggcgag ggtcacgacc cgccggtcgt
     7501 cggcggtcgc agtgtgtcgc gactcaccac cggggccgct ccagtccagg gcttgcaact
     7561 gccggagggt atgccgtgac accagaccgt gcccatcgct cgtccggtcg acggaccggt
     7621 catgcagaca gacgaggtgc ccgtcgaggg tcatccggac atcgcattcg atggcgtccg
     7681 cgccatcacg gatggcctgc cggaaggcgc caagggtgtg ctccgggcgg atcgccgagg
     7741 cgccgcggtg ggcgatcacc gccggacgac gttccttgac gtctccgccc tgcggcatcc
     7801 gaccaacctc ctcgcgacgc ccggcaacga cagcgcctca gcaacgacac cgcctcaaca
     7861 gcgacgcctt atcgtgacac ggtgcctccc atcgccggtg ctcagtcgag cgcacagtcc
     7921 actggagcgc cgacgactca cacctttcgg cgcccaggcg ttgttctcac gtgaccctgc
     7981 cgccgttcca acttctcgtc gatgcccact ggcgtgacgt cgcccgattg gctcatgccc
     8041 tcgtgggtcc ggacgacggc gacgacgtgg cgcagcaggc gtggctgcag gcgtgggcgg
     8101 catacccctc gctcacctca gccacgaacc tgcgcggctg gctgttcaca gtgacctatc
     8161 gctgcgcgac cgacgcccat cggggacggg ggcggcggcc ggtgccggtt gaggatccgg
     8221 ccctggctgc ggccgagcgc ccggccgttg acccgcccgc gaccgcctcc gacgagtccg
     8281 gcgtttggca gatcgtcgcc accctgccgc ctcgccagcg cgaggccgtc gcgctgcggt
     8341 acgtcgccga cctcgatcac gctgccatcg ctgccgcgct ggggacctcg acggcgatga
     8401 gtcgccgcct ggtcagcgac gcgctcggct ccctccgaaa ggccctctca tgaccccgtc
     8461 acactccggt ccctccgatc gactcgaagc atccctcgct ggtttcgacg tgcctgcggc
     8521 gcgcccaccg gacttcccga ccgccgacgt gtcgtatgcc gtcgacgaca ccccaatcgg
     8581 tcgaatgctg ttggcctgcg atggttctgg tgtcttggtt gcatcgctgt tcacccccga
     8641 tgccgccgcc gaagaggcag cgctccaacg gcttgcggcc cgcatcagcc cgcgggtgct
     8701 gcgtcgcccg cagtcgctgg acgaggctcg acgctggctg gagagctacc tcgacggagg
     8761 ccgccccggg gccgctcccg cgtgggcgct ctcgctcgcg accccgttcc agatccccgt
     8821 gctcacgacc ctcgccgccc gcgtcggtta cggcgatcgc gcgacctacg gccaactcgc
     8881 cagctgggca ggccgccccc aggccgcacg ggccgtcggc accgcgctcg gaggcaaccc
     8941 gctgtgcgtc gtgctcccct gccaccgcgt cgtcggtgcc agtgggtcac tcactgggta
     9001 tgccggtggg ctggccgcca agcgctacct cctggagttg gagtccacga gtctctgacg
     9061 gccgcgtgcc cgactcttgg gtcacccctc ctcagcgccc gcttctacgg tgcggaatac
     9121 tcaggcaact gactttcttc tcagtgacac tggggaagcg cttcgacgcc cggcggggga
     9181 gccgggtcaa ccgggaggaa cgaacgtgat gactgcaagg acctcaccca cacccgcgcc
     9241 gacgccgctg gacgacgatc gggtcaccga gttggtgacg cgcgcggcat acgagcggct
     9301 gcggagcgac ctcgccgtcg cgcatggcac cgagttggtg tcgtgggatg agcagaaccc
     9361 gaagatgcgc ggggacctgc gcgagtcggt cgagcccacc gtccaggaca cgatcgaggc
     9421 gattgccgag ttgggcttcg cggtcgtgcc gtacgagtgg ctcggcggtc aggcgagcga
     9481 cgccaccgcc gtcgtcatcg gcgaagccac gctcagcccc gctgcgacaa cgccgaacga
     9541 ggtcgacatc gacctgccgg ccagccctca gcgtctgctc accttggcgt cgatgacgtc
     9601 acgcctgacc tcgtcgacga acgccaccgc gccgggcgag caggtcgccg acgagagcga
     9661 gcccgtcgac cccagcgccc tgcgcgggct cgtctccggc gccgggtcag cggcagtggc
     9721 catcgtccgg ctgccgcaga ccgccctccg cccgatcaag cgggtcgtgg cccgggtcat
     9781 gggcccggcc gcctgacctc gcgcctcggt cgtcggcacc ctcgcctcac aggtcaatcg
     9841 cgaggttgaa ccagatcgac gtgacctcca tgccgacctt ctcgtagagc cccagcgccc
     9901 ctgtccgcga gtcggtcgac aactcgcaca cggtcgctcc gtgcgccctg gccgcggcga
     9961 acgagtcgac caatagcgcc cgcgccaggc cctggttgcg gacgtccttg cggacggcga
    10021 gtttgtcgac gtagccaacc tcatcggcca gcaacacgag cgacacccca accacggcac
    10081 cactcggatc gatcgccagc cgcaggttcc acggctcgaa cccgggtcgc tccatggtga
    10141 cggcagccca gtcctcgtag ggcgtgcgag ggcgctcgga ccattcgaga aacgcatcct
    10201 cgatcacttc ccaggcggcg cggtggtcag ctgccgtctc ggctatccgt agcgagaaac
    10261 cctgcggcag aggctgactc gcgatctcac gtccctgcgg cacctcaagg atccatgagg
    10321 tccaccgctg gtgatatccc agcgctcgca gcagcgcctc gctcggggag ccggcaggca
    10381 ccggcatacc aacgacactc accccggccg cacgtgccgt cgcctggatc cacctcgcca
    10441 ccgcggtccc caccccacgc ccacggtatg ccgggtgcac agccgcgtcg gagcggtcac
    10501 cacccgagtg ctcgccgtag ccgaccatgc ggtcgccgtc gaagaccccg atggtcttgg
    10561 ccgcgatgtc gaagctcggc cgttgccagt cggcggcgat gtcggcctct tcgatggcga
    10621 ccttgcccgc gtcagccaac tcgacggccg ccatgacctc gaagaccgcc cggacgtccg
    10681 aggggccgag tggccgggag gtcagcccgt caggaagcga gagcgaggcc gcccctgcgg
    10741 tggccgtctg gtcgtttgct ggggtgacgt cgtcggtcat ggccccagga tcgcaggcgc
    10801 gccacgacgc tgtcaggtga gtttcgcccg cgctcggccg ccctcgcagg cccctggctt
    10861 gaggtgtggt cgaacgcggt ccagggacca cgttcaacca cacctagggc agatctaaag
    10921 gcgaggatcg acgggttccg attccatcgc cagcaccgca aacaccgcct catgcacccg
    10981 ccacagcggc tcaccctgga ccagcctgcc gagggactcc agcccaaggg cgtactcccg
    11041 aagtgcgagc gagcgcttgt gcccaagttt ccgatcacgc agccgagtca ggtgctccgg
    11101 catcgtgtag tcgggtccat agatgatccg caggtactcc cgcccacgca ccttcagccc
    11161 tggctgcacc agacccttgg tgttccgcgt cagattcgcc aggggtttga cgaccatccc
    11221 ctcaccccca gccgccgtga gttcctccca ccaggcaacg ccctcggcgc gcgacactgc
    11281 gtcaccgacg ttgaccacga gacgcctggt cgaccggaag agggcagcat cagcctcgac
    11341 caacttgtcg gcaatctcca agtgccacaa gtgatcccgc gactcccagg tcgccccttc
    11401 gctggccagg atctgaaacg gcgcgaggga cacaccctct agcccatcca cctcccagca
    11461 atatcggcgg taggcctgcg cgaaggcatc ggcgttggcg cgacgcagct cgttacgtgc
    11521 caggagatct ccgacgtcga gcccccgcgc cgccgccgcg cgaagcgacg ccgaggccgc
    11581 ggagagcgaa ccgcgggcag cggccccgac ggcggcatac tgctcgcgga tcaacggctg
    11641 ggccttgacc gaccacggaa gcacctcggc gtccagaagg agccagtcag tggacaactc
    11701 gtcccacaaa ccggccttcg tcaccgcgtt cgccagcgac gtcagccagg ccgccgattg
    11761 ctccgggccg aagatggacc gacccgtccg cgagtaggca acgccgatct cacctctgtc
    11821 ggcaccgaat cgccttgccg cgacggcggg ttcgcggcat accagaacga cgacgcgcga
    11881 gcccatgtgc ttctcctcgc acacgacgtc cccgaccccc cactggacgt atgccgcgaa
    11941 cgcctcatca gggtgttcga gcaggccctc ccgggcggtg gtctcgaccg gtgacatggt
    12001 cggcggtagg taggccagcc agcgcgggtg catcgcgaag cggctcatca cctccaacgc
    12061 tccggcggca gcatcgtccc gcacggagat ccgcccgtga tgactggtct cgatgactcg
    12121 acgtccgagg acgtccgtga gatcgagatc ctcccggcct cgcgtcgggt cgacccgcgc
    12181 ctgggtgccc gacagagagt cgggatcggc cgtcgggaag ggacggctgg gttcgtacca
    12241 gacccgctcg gcgggaacct gaacaacctc acgctcgggc caacgcagcg cggtgaggtg
    12301 tccgccgaac acgcaaccgg tgtccaggca catcgtcccg ttgacccatt cgggcgtgac
    12361 cgtcggcgtg tggccataga gcacggtggc ccgcccccga tagtcgagcg cccacggata
    12421 gcgcacgggc agcccgaagt cgtcggtctc gccggtggtg tcgccataca gggcgaacgc
    12481 tcgcactcgg ccggaggccc gcccgtggta gcgctcgggc agccccgcat gcgcgacgac
    12541 cagtcgccca tcatcgagga cgaggtgtga gaccaggccg tcgcaccaac gacgcacgct
    12601 ctctcggaat tcgtccgcct caacgtcgag ttgggccaga gtcgtctcca gaccgtggct
    12661 gacgcgcacc ttgcggcccg acagtgctcg caccagcttg ttctcgtggt tccccgggac
    12721 ggcaaaggcg tgccccgcgg ccgccattcc catgaccaac cgcaagaccc ccggcgagtc
    12781 tggcccacgg tcgaccaggt cgcccacaaa gacgacctgg cgaccggcct cgtgagctgc
    12841 gtcgatcgga cgcccctggg catcgcgctc cacgacgtag cccagcgcat cgagcaggga
    12901 aagcaactcg ctcaggcagc catgcacgtc accgatcacg tcaaacggtc cgtgctcatc
    12961 ccgaaggtcg ttgaagagac gatcgcgaac caccgtcgcc gcctcgatct cctcgacgcc
    13021 tcgcatcacg tggacgaccc ggaatccctc gcgggcgagg cctccgatcg agcgtcgcaa
    13081 ctgctcccgc tgacgccgga ccacatgttc gccgaagtcg cgatccggac gggttcggtt
    13141 gcgcgccaag gcgactgact ccggcacgtc cagaacgagg gccactggca gcacgtcgtg
    13201 gcgcttggcc agctcgacca gctgacggcg ggcctctcgc tgaacgttgg tggcatcgac
    13261 cacgctcaac cgaccgcggg agagccgctt ccccacgatg aagtccaaca cctcaaacgc
    13321 gtcgcccgac accgattggt cgttgacgtc gtcactcacc aagcctcggc agaagtcact
    13381 cgagacgacc tcgaacggcg cgaaatggcg cgccgcgaaa gtcgacttcc ccgagcccga
    13441 ggctccgata agcaccacga ggctcagcgc tgggatgtgc agctcgctca tccggtcacc
    13501 tccgctgcgc ggttgcgggc gactgtgtcc ccacgcgtga acaaggcgag ttgggtcgac
    13561 ggcccgaggt cggggtcgac atcgccgacg gggcggaagg acacggcata cccgtatgcc
    13621 gtcgccaccc gcgacgccca cgccccgaac tccgcgcgcg tccactcgaa ccgatggtcc
    13681 gggtggcgga gcgcgccggc ggccaggttc ggatacctga cgttgaactc gctgttgggc
    13741 gtcgtgacga tgac
//
LOCUS       DAOMZY010000042        13559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_4171_length_13559_cov_6.193868, whole genome shotgun sequence.
ACCESSION   DAOMZY010000042 DAOMZY010000000
VERSION     DAOMZY010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 13559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13559
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4171_length_13559_cov_6.193868"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PLK64_03975"
     CDS             <1..612
                     /locus_tag="PLK64_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPV78929.1"
                     /translation="GAEDAVGTDGAGDPVGPGDRPNTVGTVGAGGADGPATVVDLCTG
                     SGAIAFSLKTERPDLVVRAIEADPLAHAWAVANRDRLGLDVQVDLGDARTAYPELVGT
                     VDVVTCNPPYIPDDQVPVDPEVRDHDPALALYGGSADGLALPLAMAARAAALLRPRGV
                     LVMEHADVQGPAILRALAATGSWRDAADHADLTGRPRVVVARR"
     gene            692..1555
                     /locus_tag="PLK64_03980"
     CDS             692..1555
                     /locus_tag="PLK64_03980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPV78930.1"
                     /translation="MTSNLPMSGQTALVTGATGGIGLATAVALAGLGARVGVVGRDEK
                     RTAAAVQAVSAVPGSGGADGFLADFSDQREVRALAAEVLAAYPRLDVLVNNAGGYWSD
                     RHVSADGLEWTFAVNHLAPFLLTSLLEERLRESAPARIVTVASDAHRAGRIEFDDLQA
                     ERGYAGMRAYAQSKLANILFTRELARRLSGSGVAAYSVHPGTVRTGFAREDAGRLFGL
                     VLRAGALLMKSPEQGAQTSVYAASSPELVGRTGEYLAKSRVAKPSARAQDDVAAARLW
                     SVSEDLVAGSA"
     gene            1625..2431
                     /locus_tag="PLK64_03985"
     CDS             1625..2431
                     /locus_tag="PLK64_03985"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPV78931.1"
                     /translation="MPEVFDCSAADGLALGVAAAAQAVRDGQVVVLPTDTVYGIGCDA
                     FDASAVAAVLAAKGRGREMPPPVLVPGVRTIDGLAREVPDYARALIDRFWPGALTLVL
                     RAQSSLQWDLGDTNGTVAVRMPDDPVALALLNEIGPMAVTSANRTGMPPATTAAEAQS
                     QLGESVTVYLDGGPSSGGPASTILDCTGAEPIVLREGAVAEAEIREVVAAIRAAADQA
                     AAEAAASVALEQAKRRKITEQEQAERDRAADPRRRPGSAKKVAGSRRRPN"
     gene            2753..4027
                     /gene="glyA"
                     /locus_tag="PLK64_03990"
     CDS             2753..4027
                     /gene="glyA"
                     /locus_tag="PLK64_03990"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPV78932.1"
                     /translation="MSEFYGSDFDALTAFDPDIAGVLLSELARIRGGLQLIASENISS
                     PAVLTALGSTLSNKYAEGYPGRRYYGGCAEVDKAEQIAIDRCAALFGADHANVQPHSG
                     ASANQAVYGAFLKPGETILAMSLPHGGHLTHGSKVSFSGKWFNAVHYGVDKDTEDIDY
                     DQVEALAKEHRPKVILAGGSAIPRLIDFARFRAIADEIGAIFWVDAAHFIGLVAGKAI
                     PSPVPYADVVSFTTHKVLRGPRSGAIVCTAQHAAAIDKAVFPMMQGGPQMHTIAAKAV
                     NFKECGTPEYAEYAAQVVRNAAVLATELGEHGIRPTTGGTDTHLSLHDLQGVGVTGKD
                     AEARADAAGIVLNKNAIPFDPAPPSVASGIRVGTPCVTTQGMGEEEMRRIARLIATAV
                     TKGDADPEHAVSKAVRAEVTELVTAFPAYPRP"
     gene            4079..5179
                     /locus_tag="PLK64_03995"
     CDS             4079..5179
                     /locus_tag="PLK64_03995"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009480935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HPV78933.1"
                     /translation="MREYLLICVVALAVTYLMTPFVRALAVVIKAVTPLRERDVHTVP
                     IPRLGGVAIFAGLAAATVVARQLPYLKGVYVSGQMTGVLLGAAVICLIGAIDDVRELD
                     WMTKFAGQVLAAGVMAYKGVIMLHVPFFGTSTVLPQPVLIGVTVLIVLVTTNAVNFID
                     GLDGLAAGIVAIAALAFFIYSYLLSYTFNPPNVFSTATFVTAATLGCCLGFLPHNFNP
                     ARLFMGDSGALLLGLLLAAATISITGNVDPSAVSGDQVTAALLPIIAPLAVLSIPFLD
                     MVLAVFRRARAGQKPWHPDSKHLHHRMLQIGHGHRGAVLILYLWAAVLAFGTVSWAIV
                     GGWPTLAGIAVAVVVAVTLTAYLPRWMRNRRL"
     gene            5232..5699
                     /locus_tag="PLK64_04000"
     CDS             5232..5699
                     /locus_tag="PLK64_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78934.1"
                     /translation="MTPTPGPLDGATAGPADEARESDAFAELLRGAFRPTALVGVLAV
                     VGSVFSGVTAAWSAALGAAIVLGFFTASLLVMKRTAHLPPTTVMAVVMITYTVKVIVL
                     GIVLFAVRDASWLNGYAAGVTITVCALVWLFFEMRAYKRLRIFAFSPDEKAGA"
     gene            5784..6038
                     /locus_tag="PLK64_04005"
     CDS             5784..6038
                     /locus_tag="PLK64_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HPV78935.1"
                     /translation="MSPTPPSPPSGGTPEEREALRDFNVREADSAWKAVAYLITGPAI
                     YGGLGWLLDRWWGTSFMTPVGIVGGMALSIYLIWFRYGSH"
     gene            6157..6948
                     /gene="atpB"
                     /locus_tag="PLK64_04010"
     CDS             6157..6948
                     /gene="atpB"
                     /locus_tag="PLK64_04010"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009480932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HPV78936.1"
                     /translation="MSIIALGMEGGGDGFTAPDASIFWEPLIGEGATAITRPVFLMVI
                     AGALVAWFLLATTKRRALVPSKGQMMTEGAYGLVRNSIAKDIIGSHEFLRFVPLLFTM
                     FLLILVNNFFGVIPPFNYPTMSRIGFPVALALVIWVVFHVVGMKKHGFVGYWKSLVPS
                     GLPGGMVPAIFILELITDLFTRPVTLGLRLFGNMFAGHILMALFISGGWYMLQAGGIL
                     PVAGAVTWAFAIVMTLFEMLVEFLQAYVFTLLSALYIAGAVADSH"
     gene            7074..7283
                     /gene="atpE"
                     /locus_tag="PLK64_04015"
     CDS             7074..7283
                     /gene="atpE"
                     /locus_tag="PLK64_04015"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HPV78937.1"
                     /translation="MTGNIAILGYGLSAIGPGIGVGLIFAAYINGVARQPEARGMLQP
                     IAFLGMAITEALAIFGIALAFVFKV"
     gene            7340..7921
                     /locus_tag="PLK64_04020"
     CDS             7340..7921
                     /locus_tag="PLK64_04020"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HPV78938.1"
                     /translation="MLTIPASGGEGEGGFWATAAPIIPHPIEFLFSLVVFAGLYWVVA
                     KKVVPRLEQIYAERTAAIEGGMARAEQAQAEAKAALDSYNAQLQDARGEANRIREDAR
                     AQGAQIVAEMRTQAQAEASRIVEAAQRQIEAERQSAVTSLRGEVGRLSTDLASRIVGE
                     SLQDEVRQKGIVERFLAELESGDIKPERVGGDV"
     gene            7921..8745
                     /locus_tag="PLK64_04025"
     CDS             7921..8745
                     /locus_tag="PLK64_04025"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HPV78939.1"
                     /translation="MQGSSRASAAHGQRALDALLAGGPGRAVEPAALADDLFGVCGAL
                     ESSAALRRALTDPTREASAKGELVRRLFADKVGAPAVQLVTSVVGQRWSTERDLVDTV
                     EDFAVQALASGALARGGADAVEDELFRFERIVAGTPSLRDAVTDRRATPAARAEVIQA
                     LLAGKAQPETLRLARQAVLGARGRRFDRVIAGYLAIIATRREQLSATVTSAIDLDADQ
                     SQRLAAALTRIYGKPVHLKVVLDPQIVGGIRVQIGDEVVDGTVLRKLEGARRHLGG"
     gene            8836..10476
                     /gene="atpA"
                     /locus_tag="PLK64_04030"
     CDS             8836..10476
                     /gene="atpA"
                     /locus_tag="PLK64_04030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HPV78940.1"
                     /translation="MTELTIRPEEIRDALDQFVQSYDPGAASREEVGRVSDAADGIAH
                     VEGLPSAMTNELLQFEDGTLGLALNLDVHEIGVVVLGDFAGIEEGQEVKRTGEVLSVP
                     VGDAFLGRVVNPLGQPIDGLGEIKAEARRALELQAPTVMERKSVHQPLQTGLKAVDAM
                     TPIGRGQRQLIIGDRQTGKTTVAVDTIINQKRNWESGDPEQQVRCIYVAIGQKGSTIA
                     AVRGTLEDAGALEYTTIVAAPASDAAGFKYLAPYTGSAIGQHWMYAGKHVLIVFDDLS
                     KQAEAYRAVSLLLRRPPGREAYPGDVFYLHSRLLERCAKLSDELGAGSMTGLPIIETK
                     AGDVSAYIPTNVISITDGQIYLQSDLFNANVRPAIDVGVSVSRVGGAAQIKAMKEVAG
                     RLKLDLAQFRAMEAFAMFASDLDAASRAQLAKGARLVELLKQRQSAPYPVEEQVVSVW
                     AGTTGQLDSVAVEDVRRFEVDFLDYLRREKAGLLAAIRETGKFEDSTRAGLEDAVKDF
                     KLRFFGQGGDALIEAGVEATPEDLDLADIDQEQIVKRR"
     gene            10572..11465
                     /locus_tag="PLK64_04035"
     CDS             10572..11465
                     /locus_tag="PLK64_04035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HPV78941.1"
                     /translation="MGAQMRVYRQRIRSVQATKKITRAMELIAASRVVKARQRVVESG
                     PYTHALTKALSALAEYSNEAHALITEKSEIKRAAVLVIAGDRGLSGGYNSSVIKESEQ
                     LVRKLQEEGKEVAIYLCGQRAINFYKFRHRDFVASWHGFSDAPQFDHAKEIADRLIAD
                     FSMDIDAGGNDEVHIVYTRFKSMVTQEPRVLRLVPLEVVEGEATAHPEVLPLYAFEPN
                     VEAVLDALLPKYVTNRVYTCLLGSAASQLAAQQRAMKSATDNADALIKTYTRLANQAR
                     QAEITQEISEIVGGANALASA"
     gene            11483..12943
                     /gene="atpD"
                     /locus_tag="PLK64_04040"
     CDS             11483..12943
                     /gene="atpD"
                     /locus_tag="PLK64_04040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPV78942.1"
                     /translation="MTATVAEGTATAPSEGGVGRISRIIGPVVDVEFPADSMPEQYNL
                     LTTDVELDGEKKNIYLEVAQHIGDSMVRAISLQPTDGLVRGTAVHDTGGPISVPVGDV
                     TLGHVFNTTGACLNLKEGEVLDVKERWGIHRKAPAFDQLESKTVMFETGLKVIDLLTP
                     YVQGGKIGLFGGAGVGKTVLIQEMIARVARDHGGVSVFAGVGERTREGNDLIAEMTET
                     GVMGQTALVFGQMDEPPGTRLRVALSALTMAEYFRDVQKQDVLLFIDNIFRFTQAGSE
                     VSTLLGRMPSAVGYQPTLADEMGVLQERITSTRGHSITSMQAIYVPADDYTDPAPATT
                     FAHLDATTELSREIASLGIYPAVDPLTSTSRILDRRYISEEHYNTAVRVKQILQRNKE
                     LQDIIAILGIDELSEEDKVLVNRARRIQRFLSQNTYAATQFTGIEGSTVPLADTIEAF
                     TKISDGDYDHVAEQAFFMCGGLDDVERQWAEIQKNL"
     gene            13097..>13559
                     /locus_tag="PLK64_04045"
     CDS             13097..>13559
                     /locus_tag="PLK64_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78943.1"
                     /translation="MPESAQDYYDRILAAADADGRLPLAVEAMPGWDIFPYELDSLRL
                     KPLQPLLEREPDRQGEDPASCRCAQGQPSEGVIWSDEHWRVSAERSGLPITAVLVSRE
                     HHDLADLPAELAGEFGRLLVVIAAAIEALPSVGRAHLGRYGDGGAHLHVFLF"
BASE COUNT         2038 a         4831 c         4489 g         2201 t
ORIGIN      
        1 ggggctgagg acgctgtcgg cactgatggg gcgggcgacc cagttggccc gggcgacagg
       61 cccaataccg tcgggaccgt cggagccggc ggggccgatg ggccggcgac ggtggtcgat
      121 ttgtgtaccg gctcgggggc aatcgccttc tcgctcaaga ccgaacgccc tgacctcgtc
      181 gtccgagcca tcgaggcaga ccccttggcg cacgcctggg ccgttgccaa tcgcgaccgg
      241 ctcggcctcg atgtccaggt ggacttggga gatgcgcgga cggcataccc cgagttggtc
      301 gggaccgtcg atgtggtgac gtgcaacccg ccctacatcc ccgacgacca ggtgcccgtc
      361 gaccccgagg tgcgcgacca cgatcctgcg ctggcgctct atggcggaag tgccgatggg
      421 ctcgcgctcc cgctcgcaat ggccgcgcgg gctgccgcgc tgctgcgccc ccgcggagtc
      481 ctggtcatgg agcatgccga cgtccaaggc ccggcgatcc tgcgggcgct cgcggcgacc
      541 gggtcgtggc gggacgctgc cgatcacgcc gacctcaccg gccgcccccg ggtggtcgtc
      601 gcccgtcgct agaccgctcg gcgcgccctc gtcggttccc tttcgtcggc gccgttcgac
      661 agaccgatcc gggccgtgat gtgctccgcc catgacctca aacctgccga tgtccggtca
      721 gaccgcgctc gtgaccggtg caacgggcgg gatcggcctg gcgacggcgg tggcgctcgc
      781 cgggctcggc gctcgcgtcg gcgtcgtcgg acgcgatgag aagcgcactg ccgcagcggt
      841 gcaggcggtg tcggcggtac ccgggtcggg cggtgccgac gggtttctcg cggacttctc
      901 cgaccagcgc gaggtgcggg cgcttgcggc ggaagtgctc gcggcatacc cccgcctgga
      961 cgtgctggtc aacaatgccg gcggctactg gagcgatcgg cacgtgagcg ccgacggtct
     1021 ggagtggacc ttcgcggtca atcacctcgc cccgttcctt ctgaccagcc tgctcgagga
     1081 gcgtctgcgc gagagcgcgc ccgcgcggat cgtcaccgtc gcctcggatg cccatcgggc
     1141 cgggcggatc gagttcgacg acctgcaggc ggagcgcggt tacgcgggca tgcgggctta
     1201 tgcccagtcc aaactggcca acatcttgtt cacgcgcgag ttggcgcggc ggctgtccgg
     1261 ctccggcgtg gccgcctact cggtgcaccc gggcacggtg cggaccgggt ttgcccggga
     1321 ggacgccggt cgcctcttcg gcctggtcct gcgagcgggc gcgttgctga tgaagtcccc
     1381 cgagcagggg gcccaaacct cggtgtatgc cgcgtcgtcg cccgagttgg tagggcgcac
     1441 gggggagtac ctggccaagt cacgggtcgc caagccgagt gcgcgtgctc aggacgacgt
     1501 tgccgccgcc cggctctggt cggtgagcga ggacctcgtg gcggggtcag cctgagggtg
     1561 cgccgggttt cgcgcgctgg ttgacgaacg cctaacggtc gccggtcgtc gtaaggtggg
     1621 cgccatgccc gaggtgttcg actgctctgc cgccgatggt ctggcgctcg gtgttgcggc
     1681 agcagcccag gcagtccggg atggtcaggt cgtggtgttg ccgaccgaca cggtctatgg
     1741 gatcggatgc gacgccttcg acgcgtcggc tgtcgcagcg gtgctcgccg ccaaaggtcg
     1801 cggacgcgag atgcccccgc cggtgctcgt gcccggtgtg cgcacgatcg acgggctggc
     1861 tcgcgaggtg ccggactacg cgcgcgcgct catcgaccgg ttctggccgg gggccttgac
     1921 gctggtcctt cgggcgcagt cgtcgttgca gtgggacctg ggggacacca acgggacggt
     1981 tgccgtgcgg atgccggacg acccggtcgc cctggcgctg ctcaacgaga tcggtccgat
     2041 ggcggtcact agcgccaacc gcaccggtat gccgcccgcc accaccgctg ccgaggcgca
     2101 gtcccagttg ggggagtcgg tcaccgtcta tctggacggc ggcccatcca gcggtgggcc
     2161 ggcctcgacg atcctcgact gcaccggtgc cgagccgatc gtgctgcgcg aaggtgccgt
     2221 cgccgaggcc gagatccgcg aggtggtggc ggcgatccga gccgccgctg accaggctgc
     2281 ggcagaggcc gccgcgagtg tggcgctcga gcaggccaaa cggcgcaaga tcaccgagca
     2341 agagcaggcc gagcgcgatc gggccgccga cccgcgtcgt cgcccaggct cggccaagaa
     2401 ggtcgccggc tcccgccgcc gtcccaactg accccccggc ttggcccgga gatagcgctc
     2461 gcatggtcat ctttgcgggg ctgggccagg tttggggccg gagatagcgc tcgcatggtc
     2521 atgtttgggg ggctgagccg gggttggggc cggagatagc gctcgcatgg tcatgtttcc
     2581 gcctggatcc cggggttcgg cacggagata gcgctcgcac ggtcatcttt gcggcggggc
     2641 ggggcggggc gccgcgccac gctccgtgag cggacggggc gggaggtgcg gcacacggca
     2701 tacagtggct tcttgccggt ccccccttcc ccgagaggcg cagcgagacc ccatgagcga
     2761 gttctacggc agcgatttcg acgcactgac ggccttcgac ccggacatcg cgggcgtcct
     2821 gctgtccgag ctcgcccgga tccgcggcgg cctgcaactc atcgccagcg agaacatctc
     2881 cagccccgcg gtgctcaccg ccctgggctc gaccctctcc aacaagtatg ccgagggcta
     2941 ccccggccgt cgctactacg gcggctgcgc cgaggtcgac aaggcggagc agatcgccat
     3001 cgaccgttgt gccgcgctct tcggcgccga ccacgccaac gtccagccgc acagcggggc
     3061 cagcgccaac caggccgtct acggcgcgtt cctcaagccc ggcgagacca tcctggcgat
     3121 gagcctgccg cacggcggcc acctcaccca cggcagcaag gtctccttca gcggcaagtg
     3181 gttcaacgcg gtgcactacg gcgtcgacaa ggacaccgag gacatcgact acgaccaggt
     3241 cgaggccctc gccaaggagc accggcccaa ggtcatcctc gccggcggct ccgcgatccc
     3301 gcggctcatc gacttcgcgc ggttccgcgc catcgccgac gagatcggtg cgatcttctg
     3361 ggtcgacgcg gcgcacttca tcggcctggt cgcaggcaag gcgatcccca gcccggtgcc
     3421 ctacgccgac gtcgtgtcct tcacgaccca caaggtgctg cgcggccccc gttcgggcgc
     3481 gatcgtctgc accgcgcagc acgccgccgc tatcgacaag gccgtcttcc cgatgatgca
     3541 gggcgggccg cagatgcaca cgatcgcggc caaggcggtc aacttcaagg agtgcgggac
     3601 cccggaatac gcggagtatg ccgcgcaggt cgttcgcaac gcggctgtcc tcgcgaccga
     3661 gttgggtgag cacgggatcc gtccgaccac cggcggcacc gacacccact tgtcgctgca
     3721 cgacctgcag ggtgtgggcg tgacgggcaa ggacgccgaa gcccgggccg acgcggccgg
     3781 catcgtcctc aacaagaacg ccatcccctt cgaccccgcg ccgccgagcg ttgcctcggg
     3841 catccgggtc ggcactccgt gtgtgaccac ccaaggcatg ggggaggagg agatgcgtcg
     3901 gatcgcccgg ctcatcgcca ccgccgtcac gaagggcgac gccgaccccg agcacgccgt
     3961 ctccaaggcg gtgcgcgcgg aggtgaccga gttggtcact gccttcccgg cataccctcg
     4021 cccctagtcc tccaccgccc tgctgggcga tcccgaccag acgaagcggc caccctctgt
     4081 gcgcgaatac ctgctcatct gtgtggtggc gctggccgtc acctacctga tgacgccgtt
     4141 cgtccgcgcc ctggccgtgg tcatcaaggc ggtcaccccg ctgcgggaac gcgacgtgca
     4201 caccgtgccg atcccgcggc tgggcggggt ggccatcttc gctgggctcg ctgcggcgac
     4261 cgtcgtcgcg cgccaactgc cttatctcaa gggcgtttac gtcagtgggc agatgaccgg
     4321 cgtgcttctg ggcgctgcgg tgatctgcct catcggcgcc atcgacgacg tccgtgagtt
     4381 ggactggatg acgaagttcg ccggtcaggt gctggccgcg ggggtcatgg cctacaaggg
     4441 cgtgatcatg ctgcacgtgc cgttcttcgg cacgagcacc gttctgccgc aaccggttct
     4501 catcggtgtc accgtgctca tcgtgctcgt gacgaccaac gcggtgaact tcatcgacgg
     4561 ccttgacggg ctggcggcgg gcatcgtcgc catcgcggcc ctggcgttct tcatctactc
     4621 ctacttgttg agctacacct tcaacccacc gaacgtcttc tccactgcga cgttcgtcac
     4681 cgcggcgacc ctgggctgtt gcctgggctt cctgccgcac aacttcaacc ccgcccggct
     4741 cttcatgggc gattcgggcg cgctgctgct cggcctgctc ctcgccgccg caacgatctc
     4801 gatcaccggc aacgtcgatc ccagcgccgt cagcggcgac caggtgacgg cggccctgct
     4861 gccgatcatc gccccgctgg ccgtgttgtc gattccgttc ctcgacatgg tgctggccgt
     4921 gttccggcgc gcccgggccg gccagaagcc gtggcacccc gacagcaagc atctgcacca
     4981 ccgcatgctg cagatcggcc atgggcaccg aggagcggtc ctcatcctct atctgtgggc
     5041 cgcggtcctt gcgttcggga cggtgtcgtg ggcgatcgtc ggcgggtggc ctaccctggc
     5101 cggcatcgcc gtggcggtcg tcgtggcggt gacgctcacg gcatacctgc cgcgttggat
     5161 gcgcaaccga cgcttgtgat acctttcaca agctcccctc gcaggcccca cgaccacccc
     5221 caaggcggac gatgaccccc acccccggac cgttggacgg cgccaccgct ggcccggccg
     5281 atgaggcgcg cgaaagcgac gccttcgccg agttgctgcg cggggccttc cgtcccacgg
     5341 ccctcgtcgg agtcctggcg gtcgtcggat cggtgttctc gggcgtcacc gcagcgtggt
     5401 cggccgccct cggcgcggcc atcgtcctgg gcttcttcac cgccagcctg ctggtcatga
     5461 aacgcaccgc gcacctgccg ccgacgaccg tgatggctgt ggtgatgatc acctacaccg
     5521 tcaaggtgat cgtcctcgga atcgtcctct tcgcggtccg cgacgcctcc tggctcaacg
     5581 ggtatgccgc gggcgtcacc atcaccgtgt gcgcgctggt gtggttgttc ttcgagatgc
     5641 gcgcctacaa gcggctgcgc atcttcgcgt tctcgccgga tgagaaggcg ggggcgtgat
     5701 cctgctcacc gaccccctcc ggtatgccgc gcacacccgt gcgcgggccc ggcacgacgg
     5761 ccgtctgcga gaggttttca gccatgtctc cgacccctcc atcgccccct tccggcggca
     5821 cccccgagga gcgagaagcg ttgcgcgact tcaatgtgcg cgaagcggac tcggcatgga
     5881 aggctgtggc gtacttgatt acgggacctg cgatctacgg ggggctgggg tggcttctcg
     5941 atcgttggtg gggcacgtcc ttcatgactc cggtaggcat cgtcgggggc atggcgctgt
     6001 cgatctacct catctggttc cggtacggtt ctcactgaaa ccggttctga cctatccctg
     6061 cttcgcggcg ccctcggcgg cttcgcaaca gccctcggaa cgaacgggtt tcagccgaag
     6121 cggccacccg gtcctgacca ctcgaaggag agtctcgtga gcatcatcgc tctcggtatg
     6181 gagggtgggg gcgacggctt cactgctccc gacgcctcga tcttctggga gccgctcatc
     6241 ggtgaaggag ccaccgcgat cacgcggccg gtcttcctca tggtgatcgc tggagccctc
     6301 gtggcctggt tcctgctggc cacgaccaag cgccgcgcgc tcgtgccgag caagggtcag
     6361 atgatgaccg agggcgccta cggcctggtg cgcaacagca tcgccaagga catcatcggc
     6421 agccacgagt tcctgcgctt cgtgccgctg ctgttcacga tgttcctgct gatcctcgtc
     6481 aacaacttct tcggcgtcat cccgccgttc aactacccga cgatgagccg catcggtttc
     6541 ccggtcgcgc tggctctcgt catctgggtc gtcttccacg tcgtgggcat gaagaagcac
     6601 ggcttcgtcg gctactggaa gtcgctggtc cccagcggtc ttccgggcgg catggttccg
     6661 gcgatcttca tccttgagct catcaccgac ctgttcaccc ggccggtcac gctcggcttg
     6721 cgactgttcg gcaacatgtt cgccgggcac atcctcatgg cgctgttcat ctccggcggc
     6781 tggtacatgc tgcaggccgg ggggatcctc cccgtcgctg gcgccgtcac ctgggcgttc
     6841 gccattgtca tgaccctgtt cgagatgctg gtcgagttcc ttcaggccta cgtcttcacg
     6901 ctgctgtccg cgctctacat cgccggcgcc gtcgccgact cgcactgacc tgctcgacct
     6961 gcttgacccg cactgacgtt gtttgaaccg cactgacctg cacgactcag accacccgat
     7021 tgaccccacc gggctcaccc ggttggacca aacgaaaagg aaatggaatc gacatgaccg
     7081 gcaacattgc gatcctcggt tacggcctct ccgccatcgg ccccggcatc ggtgtcggcc
     7141 tgatcttcgc cgcctacatc aacggtgtcg ctcgtcagcc cgaggctcgt ggcatgctcc
     7201 agccgatcgc cttcctgggc atggccatca ctgaggcgct cgcgatcttc ggtatcgccc
     7261 tcgccttcgt cttcaaggtc tgacccgcac cctccgctcg tccatccgtg cgcttgtccg
     7321 tgaggagaca cccgtgcaca tgctgaccat ccccgcttcc ggcggcgagg gcgagggcgg
     7381 gttctgggcc accgcggccc cgatcatccc ccacccgatc gagttcctct tcagcctcgt
     7441 cgtcttcgcg ggcctctact gggtcgtcgc caagaaggtc gtgccccggc tggagcagat
     7501 ctacgccgag cgcaccgccg cgatcgaagg cggcatggcc cgcgccgagc aggcccaggc
     7561 cgaggccaag gccgcgctcg actcctacaa cgctcagttg caggacgccc gcggcgaggc
     7621 caaccggatc cgtgaggacg cccgggccca aggcgcgcag atcgttgccg agatgcgcac
     7681 ccaggctcag gccgaggcct ctcgcatcgt cgaagcggcg caacgccaga tcgaggccga
     7741 gcgccagagc gcggtgacct cgctgcgtgg cgaggtgggc cgcttgtcga ccgacctggc
     7801 cagccggatt gtcggcgagt cgctgcagga cgaggtccgc cagaagggca tcgtcgagcg
     7861 cttcctcgcc gagctcgagt ccggcgacat caagcccgag cgcgtaggcg gcgacgtctg
     7921 atgcagggat cctcgcgcgc gtcggccgct cacggccagc gagccttgga cgcgctgctg
     7981 gccggtggac ccggccgggc cgtcgaaccg gccgccctgg ccgacgacct cttcggcgtg
     8041 tgcggtgccc tcgagtccag cgctgctctg cgccgggccc tgacggaccc cactcgtgag
     8101 gccagcgcca aaggcgagct cgtgcggcgg ctgttcgccg acaaggtcgg agcgcccgct
     8161 gttcagctcg tgaccagcgt ggtcgggcag cggtggtcca ccgagcgcga cctggtcgac
     8221 acggtcgagg acttcgccgt ccaggccttg gcgtccggag cactggcccg cggcggcgcc
     8281 gatgccgtcg aggacgagct gttccggttc gagcggatcg tcgctggcac cccgtccctt
     8341 cgtgacgccg tcaccgaccg gcgggccacg cccgccgcac gtgccgaggt catccaggcg
     8401 ttgcttgcgg gcaaggcgca gccggaaacg ctgcggttgg cccgccaggc cgtcctgggc
     8461 gcccgcggcc ggcgcttcga ccgggtcatc gccggttacc tggcgatcat cgctactcgc
     8521 cgcgagcaac tgtccgcgac ggtgaccagc gcgatcgacc tcgacgccga ccagagccag
     8581 cgcttggctg cggccctcac ccgcatctat ggcaagccgg tccacctcaa ggtcgtgctc
     8641 gacccgcaga tcgtcggcgg catccgggtc cagatcggcg acgaagtcgt cgacggcacg
     8701 gtgctgcgca agcttgaagg ggcgcggcgc cacctcggtg gctgacccgc ccaaccccgc
     8761 tccacggcat accgcaccac acccgaacca ccggctcacc cgccgcgaca ttccgattac
     8821 aggagaagac cacagatgac ggagctgacg atccgtccgg aggagatccg ggacgcactg
     8881 gaccagttcg tgcagtccta cgatccgggc gccgcgagcc gcgaagaggt cggtcgggtc
     8941 tccgacgccg ccgacggcat cgcgcacgtc gagggtctgc cctcggcgat gaccaacgag
     9001 ctgctgcagt tcgaggacgg gacgctgggc ctcgcgctca acctggacgt ccacgagatc
     9061 ggtgtcgtcg tcctcggcga cttcgccggc atcgaggagg gccaggaggt caagcgcacg
     9121 ggcgaggtcc tctcggtccc cgtcggtgac gcgttcctcg gccgcgtggt caacccgctc
     9181 ggccagccca tcgacggcct cggtgagatc aaggccgaag cgcgccgtgc gctggagttg
     9241 caggccccca cggtcatgga gcgcaagtcg gtgcaccagc cgctgcagac cggcctcaag
     9301 gccgtcgacg cgatgacccc gatcgggcgt ggccagcgcc agctcatcat tggtgaccgc
     9361 cagaccggca agacgacggt cgcggtcgac acgatcatca accagaagcg caactgggag
     9421 tccggcgacc ccgagcagca ggtccgctgc atctacgtcg ccatcggcca gaagggctcg
     9481 acgatcgccg ccgtccgcgg cacgctcgaa gacgccggcg ccctggaata cacgacgatc
     9541 gtcgcggctc ccgcctccga cgcggccggc ttcaagtacc tcgcgcccta caccgggtcc
     9601 gcgatcggcc agcactggat gtatgccggt aagcacgtcc tcatcgtctt cgacgacctg
     9661 tccaagcagg ccgaggccta ccgcgcggtg tcgctgctgc tgcgtcgccc gccgggccgc
     9721 gaggcctacc ccggcgacgt gttctacctg cacagccgcc tgctggagcg ttgcgccaag
     9781 ctgtccgacg agctgggcgc cgggtcgatg accggcctgc cgatcatcga gaccaaggcg
     9841 ggtgacgtct cggcctacat cccgaccaac gtcatctcca tcaccgacgg tcagatctat
     9901 ctgcagtccg acctcttcaa cgccaacgtc cgccccgcga tcgacgtggg tgtgtcggtg
     9961 tcccgtgtcg gtggcgccgc gcagatcaag gccatgaagg aggtcgccgg ccgactcaag
    10021 ctcgacctcg cgcagttccg cgccatggag gccttcgcga tgttcgcctc cgacctcgac
    10081 gcggcctcgc gcgcccagct ggccaagggt gcccgtctcg tggagctgct caagcagcgc
    10141 cagagcgcgc cgtacccggt cgaggagcag gtcgtctcgg tctgggccgg caccaccggt
    10201 cagctggact cggtcgcggt ggaggatgtc cgccggttcg aggtcgactt cctggactac
    10261 ctgcgtcggg agaaggccgg cctgctggcc gccatccgcg agacgggcaa gttcgaggac
    10321 agcacccgcg ccgggctcga ggacgcggtc aaggacttca agctgcgctt cttcggccag
    10381 ggtggcgacg ccctcatcga agccggcgtc gaagcgaccc ctgaggacct cgacctcgcc
    10441 gacatcgacc aggagcagat cgtcaagcgc cgctgacggt gctcggccgg gagcacccgc
    10501 tcccggccgg cccgcaggct tgcagcacgg catacccctg acccaccgag cacgacagga
    10561 aggcggcccc tatgggagcg cagatgcggg tttaccgcca gcgcatccgt tcggtccagg
    10621 cgaccaagaa gatcacccgc gccatggagc tcatcgccgc gtctcgcgtg gtcaaagcgc
    10681 gccagcgggt cgtcgaatct ggtccctaca cccacgcgtt gaccaaggcc ctctcggcgc
    10741 tggccgaata cagcaacgag gctcacgccc tcatcaccga gaagtccgag atcaagcgcg
    10801 ccgcggtgct cgtcatcgcc ggtgaccgcg gcctctcggg cggctacaac tcctcggtga
    10861 tcaaggagag cgagcaactc gtacgcaagc tgcaggagga gggcaaggag gtcgctatct
    10921 acctctgcgg gcagcgcgcg atcaacttct acaagttccg ccaccgcgac ttcgtcgcct
    10981 cgtggcacgg attctccgac gcgccgcagt tcgaccacgc caaggagatc gccgaccggc
    11041 tgatcgccga cttctcgatg gacatcgatg ccgggggcaa cgacgaggtt cacatcgtct
    11101 acacccggtt caagtcgatg gtcacccagg agccgcgggt gctgcgtctg gtgcctctcg
    11161 aggtcgtcga gggcgaggcc acggcccacc ccgaggtgct gccgctctac gccttcgagc
    11221 ccaacgtcga ggctgtcctc gacgcgctgc tgccgaagta cgtcaccaac cgcgtctaca
    11281 cctgcctgtt gggatcggct gcctcgcagc tcgccgccca gcagcgcgcc atgaagtcgg
    11341 cgaccgacaa tgccgacgcc ctcatcaaga cctacacccg gttggcgaac caggctcgcc
    11401 aggccgagat cacccaagag atcagcgaaa tcgtgggcgg cgcaaacgcc ctcgcgtccg
    11461 cctagccgca aaggaacgac tcatgactgc cactgttgcc gaaggcactg cgaccgctcc
    11521 ctccgagggt ggcgtcggcc ggatctcccg catcatcggt ccggtcgtcg acgtcgagtt
    11581 cccggccgac tcgatgcccg agcagtacaa cctgctcacc accgacgtcg agctcgacgg
    11641 cgagaagaag aacatctacc tcgaggtcgc ccagcacatc ggcgacagca tggtccgcgc
    11701 catctccctg cagcccaccg acggcctggt ccgcggcacg gccgtccatg acaccggtgg
    11761 cccgatctcg gtgccggttg gcgacgtgac tctcgggcac gtcttcaaca ccacgggtgc
    11821 ctgcctcaac ctcaaggagg gcgaggtcct cgacgtcaag gagcgctggg gcatccaccg
    11881 caaggcgcct gccttcgacc aactcgagtc caagaccgtc atgttcgaga ccggcctcaa
    11941 ggtcatcgac ctgctaacgc cttacgtcca gggcggcaag atcggcctgt tcggtggcgc
    12001 cggtgtcggc aagacggtcc tcatccagga gatgatcgcc cgagtcgccc gcgaccacgg
    12061 tggtgtgtcg gtgttcgccg gtgtcggcga gcgcacccgt gagggcaacg acctcatcgc
    12121 ggaaatgacc gagaccggcg tcatgggcca gaccgccctc gtcttcggcc agatggacga
    12181 gccgccgggc acgcgtctgc gcgtcgccct ctcggcgctc accatggccg agtacttccg
    12241 tgacgtccag aagcaggacg tgctgttgtt catcgacaac atcttccgct tcacccaggc
    12301 cgggtccgag gtctccacgc tgctgggccg catgccctcg gccgtgggtt accagccgac
    12361 cctcgccgac gagatgggcg tcctgcagga gcggatcacc tcgacgcgtg gtcactcgat
    12421 cacctcgatg caggcgatct acgtccccgc cgacgactac accgacccgg ctccggcgac
    12481 gaccttcgcc cacctcgacg cgacgaccga gctctcccgt gagatcgcct cgctcggtat
    12541 ctaccccgcc gtggacccgc tgacctccac cagccggatc ctcgaccgcc ggtacatcag
    12601 cgaggagcac tacaacaccg cggtgcgcgt caagcagatc ctgcagcgca acaaggaact
    12661 ccaggacatc atcgcgatcc tcggcatcga cgagctctcc gaggaggaca aggtcctcgt
    12721 caaccgcgct cgccgcatcc agcgcttcct gtcgcagaac acgtatgccg ccacgcagtt
    12781 caccggcatc gagggctcga cggttccgct ggccgacacg atcgaggcct tcaccaagat
    12841 cagtgacggc gactacgacc acgtcgcgga gcaggcgttc ttcatgtgcg gtggcctcga
    12901 cgacgtcgag cgccagtggg ccgagatcca gaagaacctc tgaccccacc agccgacgcc
    12961 ccaacagcgt cgcttgagaa ggtccgtcac ggggccggtc accgcacgcg gtggccggcc
    13021 ccgcggcata ccccagcggt gttggcgtgc ccagccgacc cctgggtatg ccgtgcgcct
    13081 gggtagggtc ggggccatgc cggaaagcgc gcaggactac tacgaccgga tcctggcggc
    13141 cgccgatgcc gacgggcggc tgcctctggc ggtggaggcc atgccggggt gggacatctt
    13201 cccgtacgag ctggactcgc tgcggctcaa gccgctgcag cctctgctgg agcgcgagcc
    13261 cgaccgtcag ggcgaggacc cggcctcctg ccggtgtgcc cagggacagc cctccgaggg
    13321 ggtcatctgg agcgacgagc actggagggt cagtgcggag cgctcgggtt tgccgatcac
    13381 ggccgtgctg gtgtcgcgcg agcatcacga cctcgccgat ctgccggccg agctggcggg
    13441 tgagttcggg cggctcctcg tcgtcatcgc tgccgccatc gaggcgttgc cctcggtggg
    13501 ccgcgcccac ctcggccgat atggcgacgg gggcgcccac ctgcatgtgt tcctcttcg
//
LOCUS       DAOMZY010000043        13480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_4215_length_13480_cov_5.677914, whole genome shotgun sequence.
ACCESSION   DAOMZY010000043 DAOMZY010000000
VERSION     DAOMZY010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 13480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13480
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4215_length_13480_cov_5.677914"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="PLK64_04050"
     CDS             complement(<1..900)
                     /locus_tag="PLK64_04050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPV78944.1"
                     /translation="MTPETETVLIAVGSAAGVGAAGALGVWALARRSIRSALVAAPLV
                     VVGSVAAGVLVSSRAMFISDHDSSVVLLVVAAVAPVALVYGLVLASRVHRLDRAAAER
                     AAEAVAAHERDAAVEAQRRDLVAWASHDLRTPLAGIRAMAEAIEDGVAPAEGAYPARI
                     KAQADRMAAMVDDLLALSRIHSGSMTLAREQVSVADLVSDALATGAALAERSGVLVDG
                     AADGPVLARVDARELGRVLDNLIANAVHATPRGGRVVVHAAASATEVTIRVTDACGGI
                     PADVRERMFEPWWRGDDARTPRAG"
     gene            complement(897..1622)
                     /locus_tag="PLK64_04055"
     CDS             complement(897..1622)
                     /locus_tag="PLK64_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014921278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPV78945.1"
                     /translation="MVRVLVVDDDPTVREVVAGYLSRAGLTVDEAADGMDALARARHT
                     PPDLVVLDLMLPGLDGLEVLRRLRAERGNLPVVLLTARGEESDRVLGLEVGADDYVTK
                     PFSARELVLRVQSILRRATVAPTRAADADLLVDGDLRVDPRARTVGRGGIPLPLTSRE
                     LDLLIHLLRHPGQAFSRTDLLRQVWGWEYGDESTVTVHVRRLREKVEPDPAAPQRIAT
                     VWGVGYRWDAQPAVGSGTAAGMP"
     gene            complement(1721..1924)
                     /locus_tag="PLK64_04060"
     CDS             complement(1721..1924)
                     /locus_tag="PLK64_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPV78946.1"
                     /translation="MHNVLRELRAQRGLSQGALGDALGVSRQTVNAIETGKYDPSLPL
                     AIKLARYFGQSVEEVFLIDGPPQ"
     gene            complement(1917..2321)
                     /locus_tag="PLK64_04065"
     CDS             complement(1917..2321)
                     /locus_tag="PLK64_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78947.1"
                     /translation="MLTHVSTLNRIFVALFLAGGVWAAIHGRWLLVAFIVVALAYAMW
                     AIRASLRGRTTDTGRLDIGQPTDERDRLLLDRALAAVGLAALAFECGLFLYGVGGPDP
                     ASGADSGLRFAGLCLVWIIANRVVVRRDATRA"
     gene            complement(2384..3322)
                     /locus_tag="PLK64_04070"
     CDS             complement(2384..3322)
                     /locus_tag="PLK64_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HPV78948.1"
                     /translation="MALPVCKPSWLRPILVANVVAEVLIIVTGGIVRLTGSGLGCPDW
                     PTCAQGSLTPVAAQDEGFHKFIEFGNRTLTGVLIVVAVLALWAVWTALPKRRMLHRLG
                     IAMIVGIFVEAVVGGITVWTGLNPVIVAFHFLATIVLVAIATAMLRGAQDGNEVDTGS
                     RTLLVPTITARLAWVTYAVGFLVLALGTIVTGSGPHSGDADAPARTGFDPRTMSWIHA
                     DVVMLFIGLLVAMLVAVRLARVGGSQVSGSEEELALLRRSWTSVLHVSLLQGVVGYVQ
                     FFTGLPIIVVLTHMLLAAVLAIALTYGTLNLYRNPV"
     gene            3398..4222
                     /gene="panB"
                     /locus_tag="PLK64_04075"
     CDS             3398..4222
                     /gene="panB"
                     /locus_tag="PLK64_04075"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPV78949.1"
                     /translation="MGYLDDDRRAKAWTVPSLAAAKASGRKLAMLTAYDASFARVLDR
                     HGVDLVLVGDSLGMVVRGLSSTISVTVADMSYHTAAVATGLSRALLVADLPFLADATP
                     ERALDAAQRLVQSGAQMVKLEGAGHKLDVIRFLVEREIPVCAHLGLTPQSVLRLGGYS
                     VQGREERAAARLHEDALAVAQAGASLLVLECVPSSVAASVTATIAIPTIGIGAGPGCD
                     GQVLVLHDMLGLDTGHRAPKFVKDFLGEGGSVAGAVAAYCAAVRDGSFPDAAHAYQ"
     gene            4233..5099
                     /gene="panC"
                     /locus_tag="PLK64_04080"
     CDS             4233..5099
                     /gene="panC"
                     /locus_tag="PLK64_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019618825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HPV78950.1"
                     /translation="MIDTITDLQALRRHILEWRRAGLRIGFVPTMGNLHAGHYSLVEL
                     ARAHADRVISSVFVNPTQFGPSEDFTRYPRTPEADTSGLEAAGCDALWLPTVEGMYPF
                     GIDLASNIRLPGVADVLEGVHRPGHFDGVCTVVARLFNQVQPDVAAFGKKDFQQLAVI
                     AQMVTDLAYPLELLRGEIVRESDGLAMSSRNQYLSPAERSTATVIHRTLRGMREEMLA
                     GTARLVVEARAGVELAEAGFEVDYTAVRTPDLVEPSAAPQVYAAPGRRVALIAARLGR
                     TRLIDNIEFELP"
     gene            complement(5052..5888)
                     /locus_tag="PLK64_04085"
     CDS             complement(5052..5888)
                     /locus_tag="PLK64_04085"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPV78951.1"
                     /translation="MAELSITWLGHSTLRIEVGGASVLTDPVLTHRVGPLSRAVPKPS
                     VRQWADPDVVLLSHLHHDHAHIPSLIRTAPAPILTGAANRAWVIRHRLPGPHPDATPD
                     DWVAITPGVGIRLVRADHESRPMPHRPNDAHGFLIRHDLPTGGHRVVWFAGDTSLYPE
                     MDRLGDLAGADIDVALLPIGGWGPRLSPGHLGPAEAAAAAALSGARHVIGIHYGTLYP
                     RGWPTWHLEWMTRPLADLAGHLSVRAPAATLHPLSPGGTANFSSRSGQLELDVVDESG
                     PA"
     gene            complement(5876..8026)
                     /locus_tag="PLK64_04090"
     CDS             complement(5876..8026)
                     /locus_tag="PLK64_04090"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPV78952.1"
                     /translation="MIQAPSRTWNSTRVVEALITAGAAVFGLGLGVFLVTGRTMALLE
                     GGIATLVLLLNEAVSRPLLRGLARRGSAVLAVVLGLLSQLVAITLVVALGIRASAGLS
                     FGVGLSLPQAATILAIDAVVISGAQWLVSSNDAEYVLGYVSRRRRRRTTGAASGLVIV
                     QLDGLGVQVFERALAAGQMPTVARWLHDGTHRLTPWWVPIPSTTPASQAAILFGDDTQ
                     IPSFRFWDRATGRLLVTNRPGDAEVIESGFDGRSGLLAGGGTAISTAFTGGADNSYLV
                     FSRALKPGGLGSGDAFVPLFSSPFLLPRTVILTVGEVIKELYQSRRQRIRQVQPRIAR
                     RFSFVVLRGLTNVFLRTMNLTIIADKIYEGAPVIFVDFVDYDEIAHHAGPERPEAMRS
                     LEGLDGILAALERVIADTPTHYEIVIWSDHGQSLGSTFEQLNGFTLGQRVDQLMDDAA
                     GGGTVVESSTGDEWGPINTLITATVNAMRRGPKSGSGSKELVLGPDRPARTAGRSSDA
                     TPDDGSVPAVIVVGGGNLGTIWFPRLSTRPTLAQIEQHWPALVTGLLATPGVGVVMAS
                     AGGDACVVLAADGVRWIGGDRDGQIEGLDPMRAYDSRGIADLHRLTRLPRCADLVLLS
                     TVDEAGMIHAFEGQVGSHGGLGGPQNQALFISPTALPLDDDLLIAAGDAGERPSFYGA
                     VPIHQQLQRWRTRLAEQQGARQGFSDEQARVWPS"
     gene            complement(8034..8813)
                     /locus_tag="PLK64_04095"
     CDS             complement(8034..8813)
                     /locus_tag="PLK64_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV78953.1"
                     /translation="MTTSRPATPLSTPGPAPARARVLAQARFELATLLANGEQLLVSL
                     VLPLGALVGLAVTTVPSLGDGRRIDLAVPGVLALCVISAAFTAQAIATGFDRRYAVLR
                     LLGTTPLGRDGLLWGKACATLGVEVLQWVVIGAVGVALGWRPVPIGLLYAVPFLLAGT
                     WAFVSLALLLAGALRAEGVLALANLLWVVFLVLGGVIVPRTVLPDALAGVVGILPSAA
                     LADGLRAALSSGAFSIGALVLLLAWGAVASAIASRTFRWSD"
     gene            complement(8810..9520)
                     /locus_tag="PLK64_04100"
     CDS             complement(8810..9520)
                     /locus_tag="PLK64_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV78954.1"
                     /translation="MTAAVDIVSLTRRFAAVTACDGMSWSAPRGSITIVLGPNGAGKT
                     TAIECAVGLQRPDDGAVRVLGIDPWLASAEHRAEVGVMLQAGGLPNGSKPLQLLRHLS
                     SFYARPADVGALAARLGIDGFARTTVRRLSGGQKQRVALAAALVGRPQVVFLDEPTAG
                     LDPHGRLDVYDLLKAEQARGATLVVTTHSFEEAATLATHLVVLADGRTVAQGSPAEVV
                     GSATLERVYFDLTRQARR"
     gene            complement(9620..10081)
                     /locus_tag="PLK64_04105"
     CDS             complement(9620..10081)
                     /locus_tag="PLK64_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78955.1"
                     /translation="MKLTSRRPRRALGAALLAAALPLVAVSTALASTTESGCTVSPLR
                     PVFDHVNSAGVKVIRYNMTVTCDAGRTVEIEQHVHEADTINADDHITTSNRLRFFGST
                     DTVTMWLENSLPDTEIGDEEMYQHVSFRVTSNGVTSPWTDWEDSPEQQFSK"
     gene            10271..11047
                     /locus_tag="PLK64_04110"
     CDS             10271..11047
                     /locus_tag="PLK64_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017823382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HPV78956.1"
                     /translation="MIHARCDFFAESLMLSTSMTVLLPQPAEGQIGMIGTRLDAPPPV
                     LYLLHGMSDDDTIWLRRTSIERYAAELGIAVVMPAVQRSFYNNEAFGQAYWTFIADEL
                     PALVGRSFKVSQEPADAFVAGLSMGGFGAFKLALHRPHQFAAAASLSGALDVAEHTFW
                     SARELSERTRIWGSESLEGTANDLVHLVSQADPATLPPLYLSCGTEDQLFEQYRAFVA
                     AAAGNQVNLTVDYRPGAHEWSYWDATIQDVLAWLPLRNRP"
     gene            complement(11169..11630)
                     /locus_tag="PLK64_04115"
     CDS             complement(11169..11630)
                     /locus_tag="PLK64_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78957.1"
                     /translation="MQLSARRARRALAAALLATALPLVAVGTAHASTTESGCTVSPLT
                     PVYTYINPAGDKVIRYNLTVTCDADRTVEIEQRVYEADIADADDLIVSFDRSRYFDTT
                     STVTMWLEKALPDTEAGQEEMYQHVRFRVESHGVTSPWTAWEDSPQQQFFN"
     gene            11845..12096
                     /locus_tag="PLK64_04120"
     CDS             11845..12096
                     /locus_tag="PLK64_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78958.1"
                     /translation="MDNNGTTFDLDQCRAAIRAATTAITELMGLLWQVEDAELGPLLG
                     TLDQLAALAEGARAALIREAESRGETLRVAGRVDVWVGR"
     gene            12402..>13480
                     /locus_tag="PLK64_04125"
     CDS             12402..>13480
                     /locus_tag="PLK64_04125"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HPV78959.1"
                     /translation="MDSIAAARDSASRRAEIRAATKTVTELVASLWQVQGAELGTLLS
                     ELDALAAVACGARVAVVGEAESRGEISASQAGSTAGWVAEHAPTLAAGSGAGQVARLV
                     RECTRPDLSSVQAAAVSGQVSVPTALTVVTEYARLKPRLREEAAPTVLQGLLDMGIAD
                     GPKGVRSLRPALLAAHGADGEFQADADTAERLVSLSRPSTDELGALTYQLVLDPVGAA
                     ALEAAIGPLSVPVPGPDGERDSRSPQLRRGQALIEVCRRATSAGDRPPAGVKTTLMLT
                     MSYEDLAARVGAATVVGSTQGGSLIAPETVRRLACDANVIPTVLGSRGEILEQGRSTR
                     LATPGQLAALWLRDAGCSFPGCTTPA"
BASE COUNT         2074 a         4391 c         4975 g         2040 t
ORIGIN      
        1 gccggcgcgc ggggtccggg cgtcgtcccc gcgccaccac ggctcgaaca tccgctcccg
       61 aacatccgcc ggtatgccgc cgcacgcgtc ggtgacgcga atggtcacct cggtggcgga
      121 ggcggcggcg tggaccacca cccggccacc acgcggcgtg gcgtgcacag cgttcgcgat
      181 gaggttgtcc aacacccgac ccagttcacg ggcatcgact cgggcaagga ccgggccgtc
      241 ggcggctccg tcgacgagga ccccggagcg ctcggccagg gcggctccgg tggccagggc
      301 gtcggacacg agatcggcca cgctcacctg ttcgcgggcg agcgtcatgc tgccggagtg
      361 gatccgcgag agcgcgagca ggtcatcgac catcgccgcc atccggtcgg cctgggcctt
      421 gatccgggcc ggataggcac cctcggcggg agcgaccccg tcctcgatcg cttcggccat
      481 ggcgcggatc ccggccaggg gggtgcgcaa gtcgtgggag gcccacgcga ccaggtctcg
      541 gcgctgggcc tccaccgctg cgtcccgctc gtgggcggcc acggcctccg ccgcccgctc
      601 ggcagccgcc cgatcgagcc gatggacccg gctggccagg acgagcccgt agaccagagc
      661 gaccggtgcc acggccgcga cgacgagcag gacgaccgac gagtcgtggt cgctgatgaa
      721 catcgcccgg gacgaaacga ggacgccggc cgcaaccgat ccgacgacca ccagcggggc
      781 tgcgacgagg gctgaccgga tcgagcgccg cgccagggcc cacacgccga gcgctccggc
      841 ggcgccgacg cctgcggcgg aacccaccgc gatgaggacg gtctcggtct cgggggtcac
      901 ggcatacccg ctgctgtgcc cgacccgacg gcgggctgcg cgtcccagcg atagccgact
      961 ccccacaccg tcgcaatccg ttgtggggca gcgggatccg gctcgacctt ctcccgcaac
     1021 cggcgcacgt ggacggtgac ggtggactcg tcgccgtatt cccaccccca cacctggcgc
     1081 aggaggtcgg tccgcgagaa cgcctggccg gggtggcgca ggaggtggat gagtaggtcg
     1141 agctcgcgag acgtcaacgg caggggtatg ccgcctcggc ccaccgtgcg cgcccgcgga
     1201 tcgacccgca ggtcaccgtc gacgaggagg tcggcatcag cggctcgcgt cggggccacg
     1261 gtcgcacggc gcaggatcga ctgaacccgc aggacgagct cacgggcgga gaagggcttg
     1321 gtgacgtagt cgtccgcgcc gacctccaac ccgaggacgc ggtcgctctc ctcgccgcgg
     1381 gccgtgagca acacgacggg caggttgccg cgttcagccc gcagccggcg cagcacctcc
     1441 aggccgtcga ggccgggcag catgagatcg aggacgacga gatccggtgg ggtatgccgt
     1501 gcccgggcga gggcgtccat cccgtcggcg gcctcgtcga cggtgagccc ggcacgggag
     1561 aggtagccgg ccacgacctc acggacggtg gggtcgtcgt cgacgacgag gacacggacc
     1621 atgaccgtca gcgtagggcg aggctcgccc ggtcagggtt gccgaatcct tagggttcgg
     1681 tgacgggcgg tctctacgag gagtgtcacg gcggagagtg tcactgcggg ggtccgtcga
     1741 tgaggaagac ctcttcgacg gactgcccga agtagcgagc cagtttgatc gccagcggca
     1801 gcgaggggtc gtacttgccg gtctcgatgg cattgacggt ttggcgcgac accccgaggg
     1861 catcaccaag cgcaccttgg ctcagccccc gttgcgcccg aagctcccga aggacgttat
     1921 gcacgagtgg catcccgtcg gacgacgacc cggttggcga tgatccagac caggcacagg
     1981 ccggcgaagc gaaggcccga atcggcgccg gacgctgggt cgggcccgcc gaccccgtag
     2041 aggaacaggc cgcactcgaa ggcaagggca gcgaggccga cggcggcaag cgcgcggtcg
     2101 agcagcagcc ggtcgcgctc gtcggtgggt tggccgatgt ccaggcgtcc ggtgtcggtc
     2161 gtgcgtccgc gcagggaggc ccggatggcc cacatcgcat aggcgagggc gacgacgatg
     2221 aaggcgacca gcagccaccg cccatggatc gcggcccaga cgccaccagc gaggaagagg
     2281 gcgacgaaga tgcggttgag ggtggaaacg tgagtcaaca tggctctgac tctatgtcaa
     2341 gtgtccttga ctgtaaaggg tgcttgactg cgagtgtcgt ggcctagacg gggttgcggt
     2401 agaggttgag cgtgccgtag gtgagggcga tcgcgagaac cgccgcgagc agcatgtgcg
     2461 tcagcacgac gatgatcggc agccccgtga agaactgcac gtagccgacg acgccctgca
     2521 acaggctgac gtgcaggacc gaggtccacg agcgccgcag gagggccagt tcctcctcgg
     2581 agccgctcac ctgcgacccc ccgacccggg ccaggcggac ggcgacgagc atggcgacga
     2641 gcaacccgat gaagagcatg acgacgtcgg cgtggatcca cgacatcgtc cgagggtcga
     2701 agccggtgcg ggcgggggca tcggcgtcac cggagtgtgg acccgacccg gtgacgatgg
     2761 tgccgagggc gagcacgagg aagccgacgg catacgtcac ccaggcaagg cgcgcggtga
     2821 tggtggggac gagcagcgtt cgcgagcccg tgtcgacctc gttgccgtcc tgcgccccgc
     2881 gcagcatcgc cgtggcgatg gcgaccagga cgatcgtggc gaggaagtgg aacgcgacga
     2941 tcaccgggtt gaggccggtc cagacggtga tgccaccgac cacggcctcg acgaagatcc
     3001 cgacgatcat ggctatgcca agtcggtgca gcatgcgccg cttcggcaat gcggtccaca
     3061 cggcccacag ggccagcacg gcgacgacga tcagtacgcc ggtgagcgtg cggttgccga
     3121 actcgatgaa cttgtggaat ccctcgtctt gcgcggcgac cggtgtgagc gacccttgcg
     3181 cgcaggtggg ccagtcgggg caccccagcc cgctgccggt cagtcggacg atgccgccgg
     3241 tgacgatgat gaggacctcg gcgacgacgt tagccaccag gatgggacgc agccaggacg
     3301 gtttgcagac gggaagagcc acgggcgaca gcgtatgccg cccgtcggca ccgcacgtga
     3361 gcgggtccgg cgggggccca ccaactacgg ttgtcgggtg ggctatctcg atgatgaccg
     3421 gagggccaag gcctggaccg tcccttcgct ggctgcagcc aaggcttccg gccgcaaact
     3481 ggcaatgctg acggcctatg acgcgagttt tgcgcgggtg ctggaccggc acggcgtcga
     3541 tctcgtgctc gtcggtgact cgttggggat ggtggtcaga ggcctgtcct cgaccatctc
     3601 ggtcacggtg gccgacatga gttatcacac ggcggcggtg gcgacggggc tgtcccgggc
     3661 cctgctggtg gcggacctgc ccttccttgc cgacgccacg cctgagcgag cgttggacgc
     3721 ggcccaacgt ctggtgcagt ccggcgccca gatggtcaag ctcgaaggcg ctggccacaa
     3781 actcgacgtc atccgcttcc tcgtcgagcg ggagatcccg gtgtgtgccc acctcgggct
     3841 caccccgcaa tcggtgttgc gcctgggggg ttactcggtg caaggccgcg aggagcgggc
     3901 ggccgcacgg ctgcacgagg atgccctcgc cgtggcgcag gccggggcca gcctgctcgt
     3961 ccttgagtgc gtgccgtcgt cggtggcggc ctccgtcacc gcaacgatcg cgatcccgac
     4021 catcgggatc ggcgccgggc cagggtgcga cggtcaggtc ctcgtgctgc acgacatgct
     4081 cggcctggac accggccacc gagcgccgaa gttcgtcaag gacttcctcg gcgagggtgg
     4141 gtcggtggcc ggcgcggtcg cggcatactg cgccgccgtc cgtgacggaa gcttccccga
     4201 cgccgcgcac gcctaccagt gaggggcctc ccgtgatcga caccatcacc gacctgcagg
     4261 cgttgcgccg ccacatcctt gagtggcgtc gggccgggct gcggatcggc ttcgtcccga
     4321 cgatgggcaa cctccacgcg ggtcactact cgctcgtgga gttggcccgc gcgcacgccg
     4381 accgggtcat ctccagcgtg ttcgtcaacc cgacccagtt cggcccgagc gaggacttca
     4441 cccgctaccc acgcaccccg gaagccgaca cgagcggctt ggaagcggcc gggtgtgacg
     4501 ccttgtggtt gccgacggtc gaggggatgt atcccttcgg gatcgatctc gcctccaaca
     4561 tccggctgcc tggggtcgcc gatgtgcttg agggagtgca tcggccgggc cacttcgacg
     4621 gggtgtgcac ggtcgtggct cgactgttca atcaggtcca gcccgatgtc gccgcgttcg
     4681 gcaagaagga cttccagcaa ctcgcggtca tcgcgcagat ggtgaccgac ctggcctatc
     4741 cgttggagtt gttgcgcggc gagatcgttc gcgagtccga cggtctggcg atgagttcgc
     4801 gcaaccaata cctgtcgccc gcagagcggt ccacggccac cgtcatccat cgcacactgc
     4861 gggggatgcg cgaggagatg cttgcgggca cagctcggct ggtcgttgag gcccgcgcgg
     4921 gagtggagtt ggccgaggcg gggtttgagg tcgactacac cgcggtgcgc acgcctgacc
     4981 tggtcgagcc gtcagcagcc ccgcaggtgt atgccgcccc cggtcgccgg gtcgcattga
     5041 tcgccgcccg gctaggccgg acccgactca tcgacaacat cgagttcgag ttgccctgac
     5101 cgcgacgaga agttcgcggt gccaccgggc gacagcgggt gcagggtggc ggccggggcc
     5161 ctgaccgaaa ggtggccggc caggtcggcg aggggacggg tcatccactc caggtgccag
     5221 gtcggccacc cgcgcgggta gagggtgccg tagtggatgc cgatgacgtg acgggcgccg
     5281 ctgagggctg ccgctgcagc ggcctcggca gggccgagat ggcccggtga caggcgcggc
     5341 ccccagccac cgatgggcag cagggccacg tcgatgtcgg caccggcgag gtcgccgagt
     5401 cggtccatct cggggtagag cgaggtgtcc ccggcgaacc acacgacccg gtgcccgccg
     5461 gtgggaaggt cgtggcggat gaggaagccg tgggcgtcgt tggggcggtg gggcatcggc
     5521 cgtgactcgt gatcggcgcg caccaaccgg atgccgacac ccggtgtgat cgcgacccag
     5581 tcgtcggggg tggcgtcagg gtgaggaccg ggcagacggt gcctgatcac ccaggcccgg
     5641 tttgcggcgc ccgtgaggat cggggcgggc gccgtccgga tcaacgacgg aatgtgggcg
     5701 tggtcgtgat gcaggtgcga gaggagcacg acatcggggt cggcccattg acggaccgac
     5761 ggcttcggca cggcccgcga caacggaccg acccggtggg tgaggaccgg atcggtgagc
     5821 acgctcgcgc cgcccacctc gatgcgcagg gtggaatggc ccaaccaggt gatgctcagc
     5881 tcggccacac gcgggcctgc tcgtccgaaa agccctgccg ggcaccttgc tgctcggcca
     5941 acctggtccg ccagcgctgc aactgctggt ggatcggcac cgccccatag aaactcggcc
     6001 gttccccggc gtcgcccgca gcgatgagca ggtcgtcgtc cagcggcagc gcggtcgggg
     6061 agatgaacaa tgcctggttc tgcggaccgc cgaggccacc gtggctgccg acctgtcctt
     6121 cgaaggcgtg gatcatcccc gcttcgtcaa ccgtcgacag cagcacgagg tcggcgcagc
     6181 ggggcagccg ggtgagccgg tgcaggtcgg cgatgccacg agagtcatag gcacgcatcg
     6241 ggtcgaggcc ctcgatctgg ccgtcgcggt ccccgccgat ccaccgcaca ccgtcggcgg
     6301 ccaacaccac gcacgcgtct ccgccggcac tcgccatcac gaccccgacc cctggtgtgg
     6361 cgagcaggcc ggtcactagc gccggccagt gttgctcgat ctgcgccaac gtgggtcggg
     6421 tcgacagccg ggggaaccag atggtcccga ggttgccgcc gccgacgacg atcaccgccg
     6481 gcaccgagcc gtcatccggc gtggcgtcgg aggacctgcc ggccgttcgg gccggtcggt
     6541 cggggccgag gaccaactcc ttggagccgg agcccgactt gggtccccgg cgcatggcgt
     6601 tcaccgtggc ggtgatcagg gtgttgatcg gcccccattc gtcacctgtg ctcgactcca
     6661 cgacggtgcc gccgccggcg gcatcgtcca tcaactgatc gacccgctgt ccaagggtga
     6721 acccgttgag ttgttcgaac gtcgagccca ggctctgtcc gtggtcggac cagatgacga
     6781 tctcgtagtg ggtgggggtg tcggcgatca cgcgttccag ggcggccagg atgccgtcca
     6841 acccctcgag cgagcgcatc gcttcgggcc gttcgggacc ggcatggtgg gcgatctcgt
     6901 cgtagtcgac gaagtccaca aagatgaccg gcgctccctc gtagatcttg tcggcgatga
     6961 tcgtgaggtt catcgtgcgc aggaagacgt tggtcaatcc gcgcagaacg acgaacgaga
     7021 atcggcgggc gatccggggc tgcacctgcc gaatccgctg gcgccgagac tgatacagct
     7081 ccttgatcac ctctccgacg gtcaaaatga cggtgcgcgg cagcaggaat gggctggaga
     7141 acagcggcac gaaggcgtcg cccgagccga gcccgccagg cttgagagcc cggctgaaga
     7201 cgaggtagga gttgtctgca ccgccggtga aggcggtgct gatcgctgtc ccgcctccgg
     7261 cgagcaaccc ggagcggccg tcgaatcctg attcgatcac ttcggcgtcc ccgggacgat
     7321 tggtgaccag gagcctgccg gtggcgcggt cccagaagcg gaacgacggg atctgggtgt
     7381 cgtcgccgaa gaggatcgcg gcctggctgg cgggcgtggt cgaggggatg gggacccacc
     7441 acggtgtcag ccggtgcgtg ccgtcgtgga gccagcgcgc caccgtcggc atttgccccg
     7501 cggcgagggc tcgttcgaag acctgcaccc ccagcccgtc gagctgcacg atcaccaatc
     7561 cgctggcggc tccggtggtc cgtcggcggc ggcggcggct gacatacccc aggacgtact
     7621 cggcgtcatt ggaactgacc agccactggg ctcccgagat cacgacggca tcgatcgcga
     7681 ggatcgtcgc ggcctgaggc aggctcagcc cgacgccgaa ggagagcccg gcgctggccc
     7741 ggatccccag ggccaccacc agggtgatgg cgaccaactg gctgagcagt cccaaaacca
     7801 cggcgaggac agcgctgcct cgccgggcca gaccgcgcaa cagcggtcgg ctcacggcct
     7861 cgttgaggag cagcaccaag gtggcgatcc caccctcgag cagcgccatc gtgcggcccg
     7921 tgacgaggaa gacccccagc cccaggccga agaccgccgc ccccgccgtg atcagcgcct
     7981 cgacgacgcg ggtcgagttc cacgtgcggc tgggcgcttg aatcatcgtc ggatcagtcc
     8041 gaccagcgga aggtgcggct ggcgatcgcc gaggcgaccg caccccaggc gagcaggagg
     8101 acgagggccc cgatcgagaa ggcgccggag ctcagagcgg ctcgcaagcc atcggccagc
     8161 gctgccgacg gaaggatccc gaccaccccg gcgagcgcgt cgggcagcac ggtgcgtggc
     8221 acgatcacgc cgccaagcac gaggaagacc acccagagca ggttggccaa cgcgaggacg
     8281 ccttcggccc ggagtgcccc ggccaggagc aacgccagcg agacgaacgc ccaggtcccg
     8341 gcgaggagga acgggacggc atacagcagg ccgatgggga ccggacgcca accgagggca
     8401 acgcccactg ccccgatgac gacccattgg aggacttcga ccccgagggt ggcgcaggcc
     8461 ttgccccaga gcagtccgtc gcggccgagg ggagtggtgc cgagcaggcg cagcacggca
     8521 taccgacgat cgaagcccgt agcgatggcc tgggcggtga acgcggcgga gatgacgcag
     8581 agggcgagga ctccggggac agccaggtcg atccggcgcc cgtcacccag gctgggcacg
     8641 gtcgtgactg ccaacccgac gagggcgccg agaggcagga cgagtgagac gaggagttgc
     8701 tcgccgttgg ccagcagggt ggcgagctcg aatcgagcct gggccagcac cctggcacgc
     8761 gccggtgccg gaccgggggt cgacaggggc gtggcgggtc tggacgtcgt catcggcggg
     8821 cctgacgggt gaggtcgaag tagacacgtt cgagggtggc tgatccgacg acctcggcgg
     8881 gcgagccctg cgcgacagtc cggccgtcgg ccaggacgac gagatgcgtg gccaaggtgg
     8941 cggcctcctc gaacgagtga gtggtgacga cgagggtggc gccgcgggcc tgctcggcct
     9001 tgagcaggtc gtagacgtcc aggcgcccat gcgggtcgag cccggccgtc ggttcgtcga
     9061 ggaagacgac ctgaggtcga cccactaggg ccgcagcgag agcaacccgc tgcttctgcc
     9121 cgcccgacag tcgccgcacg gtggtccggg cgaacccgtc gataccgagc cgagcggcca
     9181 gggcgccgac gtcggccggc cgcgcataga acgaggaaag gtggcgcagc agttggaggg
     9241 gtttgctgcc gttgggcagc ccacctgcct ggagcatgac tcccacctcg gcgcgatgct
     9301 cggccgaggc gagccagggg tcgatgccca ggacccgcac ggctccgtcg tccggtcgtt
     9361 gcaggccgac agcgcactcg atcgccgtcg tcttaccagc cccgttcggt cccaagacga
     9421 tggtgatgga gccgcgtgga gccgaccacg acatgccgtc gcaggccgtg actgcagcga
     9481 atcggcgggt gaggctgacg atgtcaactg ctgcggtcac gcttggcagt ctacgagggg
     9541 gcccgcctcg gacgcgccgc agccgccccc gctggtgtct cgggggcggc tgcggtggtg
     9601 cggtggggtg ccgtgtccgt cacttgctga actgctgctc cggggagtcc tcccagtccg
     9661 tccagggcga ggtgaccccg ttgctcgtca cgcggaagct gacgtgctgg tacatctcct
     9721 cgtcgccgat ctcggtgtcg ggcaacgagt tctccagcca catcgtcacc gtgtccgtcg
     9781 agccgaagaa gcgcaagcgg ttggaggtcg tgatgtggtc gtcggcgttg atggtgtcgg
     9841 cctcgtggac gtgctgctcg atctcgacgg tccgaccggc gtcgcaggtg accgtcatgt
     9901 tgtagcggat gaccttgacg ccggcggagt tgacgtggtc gaagaccggc cgcagcggcg
     9961 acaccgtgca gccgctctcg gtggtggatg ccagggccgt gctgacggcg accaggggga
    10021 gggccgcggc caggagggcg gcgccgaggg ctcggcgggg gcgccgagag gtgagcttca
    10081 tggtcgttcc tttcgggaga gtcccggtgg agccgatctc cgccggtgcc tcaacggtcc
    10141 cgtcgggagg gggtgcgccg cgtctgcccg gcgggggatc ctgggggtat gccgcgcggc
    10201 atacgggatg gatgtcgtcg gccagacgcg ccgcgcgtcc gtggcggccg aacccgctag
    10261 cgtcggcctc atgatccatg ctcggtgcga cttcttcgcc gaatccttga tgctgtccac
    10321 ctcgatgacg gtgctgcttc cccagcccgc cgagggccag atcggcatga tcgggacccg
    10381 tctggacgcg cctcctccgg tgctctacct gctgcatggc atgtcggacg acgacacaat
    10441 ctggctgcgc cggacctcca tcgagcggta tgccgctgag ttgggcatcg ccgtcgtgat
    10501 gccggccgtc caacgcagtt tctacaacaa cgaagccttc ggacaggcct attggacctt
    10561 catcgccgac gaactccctg ccctggtcgg acgatcgttc aaggtgtcgc aggagccggc
    10621 cgacgccttc gtggcgggac tgtcgatggg tgggttcggg gcctttaagt tggccctcca
    10681 ccggccgcac cagttcgctg ccgcagcctc cctgtcgggc gccttggacg tggcagaaca
    10741 caccttctgg agcgcccgcg aactcagcga gcgcacgcgg atctgggggt ctgagtctct
    10801 ggaggggacc gccaacgacc tcgtccacct ggtgtcgcag gccgaccccg ccacgctgcc
    10861 gccgctctat ctgtcgtgcg gtaccgaaga ccagttgttc gagcagtacc gggcgtttgt
    10921 cgccgccgcc gcgggcaacc aggtgaacct gaccgtggac taccggccgg gggcgcatga
    10981 gtggtcctac tgggacgcca ccattcagga cgtcctcgcc tggctgccgc tgcgcaaccg
    11041 cccgtaacgg aggtttgaac tggtggggct gttcgctgct ctgggggagc cggcgccctg
    11101 acgcgccaca gccgcccccg cgagtgtcgc gggggcggct gtggcggtgc ggtggggtgc
    11161 cgtgccggtc agttgaagaa ctgctgttgc ggggagtctt cccacgccgt ccacggggaa
    11221 gtgaccccgt ggctttccac gcggaagcgg acgtgctggt acatctcttc ctggcccgcc
    11281 tcggtgtcgg gcaacgcctt ctccagccac atcgtcaccg tgctcgtcgt gtcgaagtag
    11341 cgcgagcggt cgaaactgac gatcaggtcg tctgcatcgg cgatgtccgc ctcgtagacg
    11401 cgctgctcga tctcgacggt tcggtccgcg tcgcaggtga ccgtcaggtt gtaacggatc
    11461 accttgtcgc cggcggggtt gatgtaggtg tacaccggtg tcagcggcga caccgtgcag
    11521 ccgctttcgg tggtggacgc gtgggcagtg ccgacggcga ccagcgggag ggccgtggcc
    11581 aggagggcgg cggcgagggc tcggcgggcg cggcgcgcgg agagctgcat ggtcgttcct
    11641 ttcggtgagt ttccggggga gccgttctcc gccggtgtct caacggtccc gtcgggaggg
    11701 ggtgcgccgc gtctgcccgg cgggggatcc tgggggtatg ccgcgcggca tacggtccgc
    11761 gtcggatcgt ttctgaccgc agatcgggcg ggtgctggca ttggggcgta aggcctgtcg
    11821 gtggtcaccg ttagcgtccc ggtcatggac aacaacggaa cgaccttcga cttagatcaa
    11881 tgccgggctg cgatccgtgc cgcgacgacc gctatcaccg aattgatggg ccttctgtgg
    11941 caggttgagg acgccgagtt gggtccgctg ttgggcacgc ttgaccagtt ggcggcactc
    12001 gcggagggtg cccgcgctgc tctcattcgt gaggcggagt cgcgtggaga gaccctgcgc
    12061 gtcgcaggcc gggtcgacgt ctgggtgggt cgctgagcag gcgccgaccg tgacggcggg
    12121 gggtggcggc gggacaggtc gccccgctcg gccgcaagga cgcccggcca gacctgtcat
    12181 gggttcaagc cgctgcggct aggtaccggg gtcgggtggc ttgcgtaggt cgccgacgcc
    12241 ggcgagcagc atcgcgccga gggccgggcg gacgccgggc atcgactcga tcaccctcgc
    12301 gtgggggcgg tgggatcgtt acctgaccgt gatgtgttcg attgtgggag aggccttttg
    12361 gggcgggggg tctgtcggtg gtcgctgtta gcgtcggggc tatggacagc atcgcagcgg
    12421 ctcgtgactc ggcatctcgc cgggctgaga ttcgtgctgc gacgaagact gtcactgagt
    12481 tggtggcgtc gttgtggcag gtccagggag ccgagttggg gaccttgttg agtgagctag
    12541 acgctctcgc tgcagtcgcg tgcggtgctc gggtcgcggt cgttggtgaa gctgaatctc
    12601 gtggggagat ctccgcatcg caggccgggt caacggccgg gtgggtcgcc gaacacgcac
    12661 cgactctggc tgcgggcagc ggcgcgggcc aggtcgcccg gctcgtccgc gaatgcacgc
    12721 gccccgacct gtcgagtgtt caggcggctg cagtctctgg ccaggtgagc gtgccgacgg
    12781 ctctcactgt ggtcaccgaa tacgcccggt tgaagccgcg ccttcgtgag gaagccgccc
    12841 cgaccgtcct gcaaggtttg ttggacatgg gcatcgctga cggaccgaag ggcgtccggt
    12901 cgctgcggcc ggcgttgttg gcggcgcatg gcgcggatgg ggagttccag gcggacgccg
    12961 acactgccga acgtctcgtc tccctgtccc ggccgtcgac cgacgaactc ggcgccctca
    13021 cctaccaact ggttctcgac cccgtcggag ccgctgctct ggaagcggcg atcggcccgc
    13081 tgtcggtccc cgttcctggc cccgatggtg aacgcgactc ccgtagtccg cagttgcgtc
    13141 gtgggcaggc gctcatcgag gtatgccgtc gcgccaccag tgccggtgat cggccgccgg
    13201 ctggggtgaa gaccacgttg atgctgacga tgtcctacga ggacttggcg gctcgggtcg
    13261 gcgcggcgac ggtggtcggc tcgactcagg gcggctcgct gatcgcgccg gagacggtgc
    13321 gtcggttggc gtgcgacgcg aacgtcatcc cgactgtcct gggtagccgg ggggagattc
    13381 ttgagcaggg tcgctcgacc cggttggcga cgcctgggca gttggctgcg ttgtggttac
    13441 gggacgcggg gtgcagtttc cctggttgca cgactccggc
//
LOCUS       DAOMZY010000044        13449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_4230_length_13449_cov_5.703673, whole genome shotgun sequence.
ACCESSION   DAOMZY010000044 DAOMZY010000000
VERSION     DAOMZY010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 13449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13449
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4230_length_13449_cov_5.703673"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="PLK64_04130"
     CDS             complement(<1..849)
                     /locus_tag="PLK64_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78960.1"
                     /translation="MSGLLPRPVCRPAGHSSTLATRGATPTTATRLAAIAALVVFATA
                     LGACWRDTATLHLVGTVQESLTVVAMPAIGMPTVDLDAGFAATQTSAQSQAPAQQANV
                     RTVSPTVTITGLGGVWRLATVTVREGSQVTAGQPIATVEDGILAAGVRAAEATAATAA
                     AQVPVLADRIAEVDDQRATLTDKHGQLTTALATIASSRATLTTNRTQLNANQTALQGN
                     RAGLAQTLEDLTATQTTLRAILAGLPTDPSVPLPPGTPTRAELTAQLAALATGIATAT
                     AGLAKLD"
     gene            complement(846..1475)
                     /locus_tag="PLK64_04135"
     CDS             complement(846..1475)
                     /locus_tag="PLK64_04135"
                     /inference="COORDINATES: protein motif:HMM:NF025605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPV78961.1"
                     /translation="MAKPAVNRAAAKRAQITKAAREVFLERGYAATSMDAVTALAGVS
                     KQTIYSYFPSKGELFADFLDGELGHLRVDVGAPAPITSTAQLRATLLAVATALVRRFM
                     RDDTINLLRVVLGDAYRVPEVRDAVREVIPNQLFGLVEAILGAAAAEGVVEPPQPGLT
                     ARMFIGPVVSFVILDGLLVDGPPRLPDAHALGYVVDAFLATLAPAGDRP"
     gene            1667..2146
                     /locus_tag="PLK64_04140"
     CDS             1667..2146
                     /locus_tag="PLK64_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78962.1"
                     /translation="MLVVVASSVLVGGSACGHGAGTTASEELIRLAKGSEESLVRSLI
                     GKEATARGTNADEAANLWVTRFTSAHVAYQEISPHARAVGCEAVTSWLGALATPTTDD
                     DLSAHVQVALAIANLNSVVSNRALVADLQGATERAANGEPIYLTVLMMKTGLCQVVG"
     gene            complement(2164..2841)
                     /locus_tag="PLK64_04145"
     CDS             complement(2164..2841)
                     /locus_tag="PLK64_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPV78963.1"
                     /translation="MSDPTPVRLVLVDDHAMFRSGVKAELDHARTTIVGEAPDVDRAI
                     EVIRETKPDVVLLDVHLPGGMGNGGVDVLLGCRNLTTEAGAPVRFLALSVSDAAEDVI
                     AVIRAGARGYVTKTISGTDLMRAIQRVGDGDAVFSPRLAGFVLDAFGAAAGEIAEVDE
                     ELDRLSAREREVMRLIARGYQYKEVAKELFISIKTVETHVSSVLRKLQLSSRHELTAW
                     AAGRRMI"
     gene            complement(2838..4229)
                     /locus_tag="PLK64_04150"
     CDS             complement(2838..4229)
                     /locus_tag="PLK64_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPV78964.1"
                     /translation="MTSSPPHARPPLARSSADAVVGGVCAGLGEFLGMPAVAVRAAAV
                     LLTVFGGGLGVAIYLVLWAVLPVSAAPAVIAESVEERRQPRFSVFHWLVLGGIVLVTG
                     GISLASDAGSWFTDARYAVPALAIAAGAFVAWYQLDDPTGRRRGRTRARWVGVVQVVV
                     GVALAMVGVVVIVTQGQGTRGVVNGALAGAAVLAGAVVIAAPFALRLYRGLQREQAER
                     VRATERADIAAHLHDSVLQTLALIQRRADDPTVVAGLARRQERELRAWLYGGESRESN
                     SLAAAVAAEIHEVEDEHGVPVEIVVTGDRTMEEAGDALVKATREAVLNAVRHGRPPVS
                     VFMEVGPSGVEVFVRDHGDGFEMSDIGDDRLGVRESIIGRMKRAEGTARIRRLENGTE
                     VSLTLPPPAHAEPEPEPETPPEFAPEPAPPTEEPAEPAASDDPATPEPAVTPQATARH
                     TRAASASTKENPR"
     gene            4345..5829
                     /locus_tag="PLK64_04155"
     CDS             4345..5829
                     /locus_tag="PLK64_04155"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPV78965.1"
                     /translation="MTGNPADAPMPGGPGGPAGPAGPAGPVGPSGIDVFFERLRRLGV
                     ARATQGKVAGGVCAGLARRWNIDPLVARLVFVVLTVFGGFGLLAYLVALAMLPDEKGT
                     IFAEEALRRRHGGAIFLVFIIAVMTVGEFFDRWWVWLAIPLALAAWWIVRGAAAGKSP
                     RQLGSEAKTHAAEAADAVRGWGAATPPAPAPPLSAPYQPTYQAPPPYQPYQATPVSHA
                     TPTSPAADSPWGHLPQDHPAPMTAPHGMGPGRTWSPAPQAPAPLVVREKRRRGGFLVF
                     ITALGLAAITTGTLSSTNALGDRVAQPALFAVIAGCAVAALVLFVVALRGMRAPFTAF
                     LLTLVLAVSAAAAFVPNPVGYLAGAGERSWTPVSGTGSTAYALGFGEGTLDLKGISPT
                     ADTTAGLSAQQVSVQLGFGELVVIVPDGVTARINLAIGAGDVIAEQPGGADPSSHLYS
                     GGAGQQTYLVGTGSPDVVVNVSVGFGSVRLQSTTAPSVIRDGVS"
     gene            5826..6056
                     /locus_tag="PLK64_04160"
     CDS             5826..6056
                     /locus_tag="PLK64_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78966.1"
                     /translation="MSQPPPQIIPAPRGPNSRLLIHSLLLLLGAAALLGHLAGIEFVT
                     SVSLPWPMVLIIGGAALVVIGQLGLLRRRHRS"
     gene            complement(6077..6814)
                     /locus_tag="PLK64_04165"
     CDS             complement(6077..6814)
                     /locus_tag="PLK64_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPV78967.1"
                     /translation="MTAWDLWLEGRLADGRVVGPWLLEHGNDPGITLWDNGLVMDELL
                     DVWSEAGEGRCVLTFGVREVTDGDRTPAARVPARDPDVVQAPGEVAERVQRLAAYAFV
                     HSHRGVLLAQLSDRTHAPGQWGLPGGGVDPGESPQDAVLREVAEESGQRVRLVGVLGV
                     VDGHWVGRAPDGRLEDFHAVRLVWQAVCDDPTDPVVHDVGGTTAAAAWVPIEHVASLP
                     LSDAWRGVLVDLTVPTTDGAGGSAHQT"
     gene            complement(6811..7926)
                     /locus_tag="PLK64_04170"
     CDS             complement(6811..7926)
                     /locus_tag="PLK64_04170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPV78968.1"
                     /translation="MRIAMLTDCYLPRLGGIEVQVADLSARLVARGHEVEVFTLTPGA
                     VGFGQTETLDGIRVHRLGVRLPRQLLVNPLATRGLRAQLEGFDVAHIHMGVVSPFASD
                     CGFVTSRMSLPTTMTWHCVLDKAESAVRAAGIVRRWAQSGMAMNAVSDVAATPLRRIV
                     DGPPVTVLPNGIDVDAWRPTSGRPLLGDGVVRFVSAMRLEARKRPVQLVEAMAKVRAA
                     APRAGVRLEILGEGSERAKVERTVDRLGAKDWVSLPGRVPRDTLKERYAASDVFVSPS
                     VLESFGIAALEARVAGLPVVGRVGSGISEFVTDDVNGFLANDDEELVRRLTTLAVHPS
                     VRSRMTAYNLKTDPAQGWDQVVQAAEAEYARARALAG"
     gene            7965..8708
                     /locus_tag="PLK64_04175"
     CDS             7965..8708
                     /locus_tag="PLK64_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPV78969.1"
                     /translation="MPLTLLAFHAHPDDEAILTGGTLARAAAAGHRVVLVTATDGALG
                     LTSSRYAAQGLASVRAAEVRESARLLGVSRVEQLGYADSGLGPVTLDDPPGQVRFVRV
                     PVAEAAERLAAILREESVDTLLTYDVNGGYGHPDHLQVHRVGAAAAQLAQTPRVLEVA
                     VSQRVARLMKPRGLALAPVRPVTVEVDVREQLDAKVAAMRAHESQLASDSALPRNIDL
                     LTRLPRPALERLLGRERFADLSGSSDPVL"
     gene            complement(8738..9511)
                     /locus_tag="PLK64_04180"
     CDS             complement(8738..9511)
                     /locus_tag="PLK64_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobB"
                     /protein_id="HPV78970.1"
                     /translation="MPPTSSKGRIVLVHLYPREMSIYGDLGNTRCLAARIRRHGYDVE
                     VHDHHPGMPFPQDAHLLMGGGGQDSGQSRVEDDLAVIGDRLRGLAADGTPMVMICGMY
                     QMFGHAFITSDGVELPGLGILDVTTRGQSTRMIGGIVLDTQWGEVVGFENHSGATMLG
                     SGQAPFGTVRVGHGNNGQDHTEGARTHNVIGSYLHGPILPANPAVADALIAAAAERAT
                     GRPFEPEQVDDSLADEARTRQITRLRARGTGKLKDTAVH"
     gene            complement(9514..10815)
                     /locus_tag="PLK64_04185"
     CDS             complement(9514..10815)
                     /locus_tag="PLK64_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009760531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HPV78971.1"
                     /translation="MLRTSAAIIAGKTLRYAARLRGGGSALPGLVAERVDPQILARTL
                     ASVPGGIVVVTGTNGKTTTTKMLAAIIAAHGKHVFTNPTGSNFTRGVLSAMLGEIGVG
                     GRLQADVAVLELDEWHALQFAKLVTPTHALLLNVARDQLDRFAEIDTTAKLLATLAGQ
                     TSQVVVLNRDDSFVFRIAEGLSGKEIRYFGVDPEIADQVPALNEADVRFEDAATPPPP
                     TTDDGLLHTVNTRDFAIRFGPAEQVGPLALRQRGLAAMVNATAATTMARAVLGADFRT
                     DLTEAALRTVTAPFGRGEVIDVDGHPLELVLVKNPAGFTVALSTYGAEPVATMIAIND
                     DYADGQDVSWLYDVSFASLREHGVAMTSGVRAYDMALRLDYDEVLVEQIEPDLAGALD
                     GFLAAYRGQPTRIFCTYTAMMALRKLLAARFGLAGFAQEGE"
     gene            10939..11352
                     /locus_tag="PLK64_04190"
     CDS             10939..11352
                     /locus_tag="PLK64_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78972.1"
                     /translation="MTDSPWQPQQGPFGYVRFHLQGSVWTSSLVMPTVTINGYRVPVT
                     YGENVIPVYPGRCVVEASMRWMRRYGQAGYTVDVAPGQAADIWYAAPLTQFHTGSMGV
                     AKQSRKGGWALAGMLAAIMLIFLGLPLTLLFALSR"
     gene            11407..11940
                     /locus_tag="PLK64_04195"
     CDS             11407..11940
                     /locus_tag="PLK64_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78973.1"
                     /translation="MTDIPWQPLPPDPLGPWDDLPAHSPARPPADFRPAPPAESFHQP
                     LPPKAPAEVGYLRFHMQRTWWITTGIKPILTLNGTPVKVKYGENTIPLQPGRYVVEAS
                     QVWRSHHGHASYPITIEPGEVVDVWYAGPATQYHKGSIGPSEQQREGLRNAYVLLLGV
                     LGVLYAAMFAVVLVTWN"
     gene            complement(11990..>13449)
                     /gene="guaA"
                     /locus_tag="PLK64_04200"
     CDS             complement(11990..>13449)
                     /gene="guaA"
                     /locus_tag="PLK64_04200"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPV78974.1"
                     /translation="MSVAAMLAKNPAAVILSGGPASVYAAGAPSLDPALLAAGVPVFG
                     MCYGFQAMVQALGGTVAHTGTGEYGHTDAVVTPDGSLLFDGQPKEQSVWMSHGDSVTE
                     APAGMAVTATTPGATVAAFEDADRRLFGVQWHPEVLHTAYGQRVLENFLWRGAGIPAD
                     WTASNVVESLVESVRAQVGTGRVLCALSGGVDSSVAAALVQRAVGDQLTCVFVDHGLL
                     REGEHEQVEQEFVAATGVRLVVVDARERFLTALAGLTDPEAKRKAIGAQFIRVFEQAA
                     RDLVGSASADGNPIEFLVQGTLYPDVVESGGGEGAANIKSHHNVGGLPKDLKFTLVEP
                     LRALFKDEVRAVGLELGVPEAIVWRQPFPGPGLGVRIIGEVTAERLAILRAADAIARE
                     ELTAAGLDREIWQCPVVLLAEVRSVGVQGDGRTYGHPIVLRPVSSEDAMTADWTRVPY
                     DVLAKISTRITNEVSEVNRVVLDVTSKPPGTIEWE"
BASE COUNT         2020 a         4787 c         4629 g         2013 t
ORIGIN      
        1 gtcgagtttc gccaggcctg cggtggcggt ggcgatcccc gtggcgagtg cggcgagttg
       61 cgcggtgagc tctgccctgg tgggcgtgcc gggcgggagc gggacgctcg ggtcggtcgg
      121 caggcccgcg aggatggcgc gcagcgtggt ttgggtggcg gtcaggtctt cgagtgtctg
      181 cgcgagacca gcccgattgc cctgcagcgc agtctggttc gcgttgagct gggtgcggtt
      241 ggtcgtgagc gtcgcgcggc tcgaggcgat cgtcgccagc gccgtggtga gttggccgtg
      301 cttgtcggtg agggtggctc gctggtcgtc gacctcagcg atccggtcag cgagcaccgg
      361 aacttgagcg gcggccgtcg cggcggtcgc ttccgcagcc cgcacccccg ccgcgagtat
      421 gccgtcctcc accgtcgcga tcggctgtcc cgcagtcacc tgggaacctt ctcggactgt
      481 caccgtcgcc agtcgccaca ctcccccgag gccggtgatg gtcactgtcg gcgagacggt
      541 gcggacgttc gcttgctgag ctggtgcctg gctctgggcc gaggtctgcg tggcagcgaa
      601 gccggcgtca aggtcgacgg tgggcatgcc gatggccggc atggcgacga ccgtgaggga
      661 ctcctggacg gtgccgacga ggtgcagcgt ggccgtgtcg cgccagcagg caccgagtgc
      721 cgtcgcgaag acaacaagcg ccgcgattgc cgcgagccga gtcgctgtcg tgggcgtggc
      781 gcctcgggtg gcgagcgtcg acgagtggcc ggcgggacgg cataccggac gagggaggag
      841 accactcatg gtcgatcacc tgcgggcgca agggtggcaa ggaaggcgtc gacgacgtac
      901 cccagcgcat gggcgtccgg gagtcgcgga gggccgtcga cgagcagacc atcgaggatg
      961 acgaagctga cgaccgggcc gatgaacatg cgcgcggtga ggccaggctg cggcggttcg
     1021 acgacgcctt cggccgccgc ggcgccgagg atcgcctcca cgaggccgaa gagttgattg
     1081 gggatcactt cgcggacggc gtcgcggacc tcgggcacgc gataggcgtc gccgaggacg
     1141 actcggagca ggttgatggt gtcgtcgcgc atgaaccggc ggacgagcgc ggtcgcaacg
     1201 gcgagcagcg tcgcgcggag ttgggccgtg ctggtgatcg gcgccggcgc cccgacgtcg
     1261 acgcgcagat gcccaagctc gccgtcgaga aagtcggcga acagttcacc cttgctgggg
     1321 aagtagctgt agatcgtttg tttgctcacc ccggcgaggg cggtgacggc gtccatggag
     1381 gtggccgcgt aaccccgctc gaggaagacc tcacgcgccg ccttggtgat ctgggcgcgc
     1441 ttggcggccg ccctgttcac cgcaggcttg gccatgaatc aaactgtacg gttcagtgtg
     1501 atcgagagca agagggggtc taacgtcccc acggcgctac cccaccgtcc ggaagcagcg
     1561 tcggcacgga cagggccggg gggacgcaat cctccggatc ggcagggcaa cagcgggcta
     1621 cccgcgcata ctcgacctca tgagagaccg ccgaagcatc tggctcgtgc tggtcgtcgt
     1681 ggcgtcctcg gtcctcgtcg gtgggtcggc ctgtggccac ggcgccggga ccactgcttc
     1741 cgaggagttg attcgtctcg ccaagggatc agaggagtcc ctggtgaggt ccctcatcgg
     1801 aaaggaagcc acggcccgcg gtaccaacgc ggacgaggcg gccaatctgt gggtgaccag
     1861 attcacgagt gcccatgtcg cataccaaga aatcagccct cacgcccgcg cggtcggatg
     1921 tgaggcggtc acaagttggc taggtgcttt ggcgacgccg accaccgatg acgatctgtc
     1981 ggcccacgtg caggtggccc tggcgatcgc caacctgaat agcgtggtga gcaatagggc
     2041 cctcgtggcg gatcttcagg gtgccaccga gcgcgccgcc aacggggagc cgatctacct
     2101 gacggtgctg atgatgaaga cgggcctttg ccaggtggtc ggctgacgtg cggccggccg
     2161 agctcagatc atccggcggc cggcggccca ggcggtcagc tcgtgccgac tcgacaactg
     2221 caacttccgc agcacggacg acacatgcgt ctcgaccgtc ttgatcgaga tgaacaactc
     2281 cttggccact tccttgtact gatacccccg cgcaatcagc cgcatcactt cacgctcgcg
     2341 cgccgacagc cggtcgagct cctcgtcgac ctcggcgatc tcgcccgccg ccgccccgaa
     2401 cgcatccaac acaaagccag ccagccgcgg gctgaacacc gcgtccccgt cgcccacccg
     2461 ctggatcgct cgcatgaggt cggtgccgct gattgtcttg gtgacatagc cccgagcccc
     2521 cgcgcggatc accgcgatga cgtcctcggc ggcatcggac accgacagcg cgaggaaccg
     2581 caccggcgcg cccgcctccg tggtcaggtt gcgacacccg agcagcacgt caacgccgcc
     2641 gttgcccatc cccccgggca ggtgcacatc aagcagcacg acgtcgggct tggtctcgcg
     2701 gatcacctcg atcgcgcggt cgacgtcggg cgcctcaccg acgatcgtcg tccgcgcgtg
     2761 gtcgagctcg gccttcaccc ccgagcggaa catcgcgtgg tcatcgacca gcaccaaccg
     2821 caccggggtc gggtcgctca tctcgggttc tccttcgtcg aggcagacgc agcacgggta
     2881 tgccgtgcag tcgcctgcgg ggtcacggca ggttcggggg tggccgggtc gtcagacgcc
     2941 gccggctcag caggttcttc agtcgggggc gctggctctg gcgcgaactc cggcggggtc
     3001 tccggctcgg gctccggctc ggcgtgagca ggcggcggga gcgtcagcga gacttcggtg
     3061 ccgttctcca ggcgccggat ccgggccgtc ccctcggccc gcttcatccg cccgatgatc
     3121 gactcgcgaa cgcccaagcg gtcatctccg atgtcgctca tttcgaagcc gtcgccgtgg
     3181 tcgcgaacga acacctcgac gccactcggc ccgacctcca tgaacaccga caccggcgga
     3241 cgaccgtgcc gcaccgcgtt gagcaccgcc tcgcgggtgg ccttgaccag cgcgtcgccg
     3301 gcctcctcca tcgtccgatc gccggtcacg acgatctcga ccggaacacc gtgctcgtcc
     3361 tcgacctcgt ggatctcggc agcaacagcg gcagccaggc tgttgctctc gcgggactct
     3421 ccgccataga gccaggcccg caactcgcgc tcctggcgtc gggccagccc ggcgaccacc
     3481 gtcgggtcat cggctcggcg ctgaatgagc gcaagggtct gcaggaccga atcatgcagg
     3541 tgcgcagcga tgtcggcccg ctcggtcgcc cgcacccgct cggcctgctc gcgttgcagc
     3601 ccgcgataga gccgcagcgc gaacggcgcc gcaatgacga ccgcccccgc gagcacggcg
     3661 gcaccggcga gcgcgccgtt gacaacccca cgcgtgcctt gcccctgcgt gacgatgacg
     3721 acgacgccga ccatcgccag cgcgacgccg acgacgacct gcacgacgcc gacccaacgg
     3781 gctcgggtcc ggccgcggcg acgcccggtg gggtcgtcca gttggtacca cgcgacgaac
     3841 gcgccggccg cgatggccag ggcggggacg gcatacctcg cgtcggtgaa ccacgacccc
     3901 gcatcactgg cgagcgagat gcccccggtg accaggacga ttccgccgag aacgagccaa
     3961 tggaagaccg agaaccgcgg ttgccgccgc tcctcgaccg actcggcgat gaccgcgggg
     4021 gcagccgaca cgggcagcac cgcccacagc accaggtaga tcgcgacgcc caagccacca
     4081 ccgaacaccg tgagcaggac ggccgccgcg cggacggcga cggccggcat acccaggaac
     4141 tcacccagcc ccgcgcacac gccgccgacg accgcgtccg cgctggagcg cgccaacggg
     4201 gggcgagcat gcggcggcga ggaagtcacg tccccatctt ggggcatggg tatgccgtct
     4261 gctcccctca ccggggcggg ttcagggtgc tttcagggtc caccctcatg gcagggcggg
     4321 cctccgacca gcgacgatag gggcatgacg ggaaaccctg ctgacgcgcc gatgcccggc
     4381 ggccctggcg gcccggctgg tcccgcgggt ccggcgggtc cggtcggtcc cagcgggatc
     4441 gacgtcttct tcgagcggct gcgccggctc ggagtggctc gggcaacgca gggcaaggtc
     4501 gccggcggag tctgtgcggg cctggcccga cggtggaaca tcgacccgct cgtcgcgcgc
     4561 ctggtcttcg tcgtcctcac cgtcttcggc ggcttcggcc tgctggccta cctggtcgcg
     4621 ttggccatgc tgccggacga gaagggaacg atctttgccg aggaggcgct gcgtcgccgc
     4681 cacgggggtg ccatcttcct ggtcttcatc attgccgtca tgacggtcgg agagttcttc
     4741 gaccggtggt gggtgtggct ggcgatcccc ttggcgctgg ccgcctggtg gatcgttcgc
     4801 ggcgcggctg cgggcaagtc cccgcgccaa ctggggtctg aggcgaagac gcacgctgct
     4861 gaggctgccg acgcggtccg cgggtggggc gccgcgacac cgccggctcc agcgcctccc
     4921 ctgtctgcgc cgtatcagcc gacgtatcag gcacccccgc cgtatcagcc gtatcaggcc
     4981 accccggtgt ctcacgcgac tcccacgtcg cccgccgccg actcgccctg ggggcatctg
     5041 ccgcaagacc atccggcgcc gatgaccgcg ccacacggaa tggggcccgg caggacgtgg
     5101 agccccgctc cccaggcgcc tgccccgcta gtcgtgcgcg agaagcgccg ccgcggtgga
     5161 ttcctcgtct tcatcaccgc cctggggctg gccgccatca ccacgggaac gctgagctcg
     5221 accaatgcgc tgggcgatcg ggttgcgcaa cccgccctct tcgcggtcat cgccggctgc
     5281 gccgtcgccg cactggtgct cttcgtcgtc gctctgcgcg gcatgcgggc ccccttcacg
     5341 gcgttcctcc tgacgctcgt cctggccgtc agcgccgcag ccgccttcgt ccccaacccc
     5401 gtcggctacc tggccggtgc cggggagcgc tcctggacgc ccgtctcggg aaccggctcc
     5461 acggcatacg cgctgggttt cggtgagggg accctcgatc tgaaggggat ctcccccacg
     5521 gccgacacca cggcgggctt gagcgcccag caggtctccg tgcagttggg gttcggtgag
     5581 ctcgtcgtca tcgtgcccga cggggtcacc gcccgcatca acctagccat cggagcgggc
     5641 gacgtcatcg ccgagcagcc cggcggtgcc gacccgtcga gccacctcta ctccggcggg
     5701 gcgggccagc agacctatct cgtcggcacc ggatcaccag acgtcgtcgt caacgtcagc
     5761 gtcggcttcg gcagcgtccg gttgcagagc accacggccc cgtcagtcat ccgggatgga
     5821 gtctcatgag tcagccaccg ccccagatca tccccgcccc gcgcggcccc aactcgcgcc
     5881 tgctgatcca ctcactgctg ttgctcctcg gcgccgcggc cctgctgggc cacctcgccg
     5941 ggatcgagtt cgtcacctcg gtgtcgctgc cgtggccgat ggtcctcatc atcggcggcg
     6001 ccgccctggt cgtcatcggg cagttggggc tactgcgccg acgccaccgg tcctgacggg
     6061 cggcggcccc ccaggctcat gtctggtggg ctgagccccc ggcgccgtcc gtggtcggaa
     6121 cggtcagatc cacgagtacg ccacgccatg cgtcactgag cggcaggctc gccacgtgct
     6181 cgatgggcac ccacgcggcc gcagccgtcg tcccgccgac gtcgtggacc accgggtcgg
     6241 tcgggtcgtc acacaccgcc tgccacacca gccgcacggc gtggaagtcc tccaaccggc
     6301 cgtcgggtgc gcgcccgacc cagtgaccgt ccacgacacc gagcacaccg accaggcgca
     6361 cccgctgacc gctctcctcg gcgacctcac gcaacaccgc atcttgcggc gactcccccg
     6421 ggtcgacacc accaccgggc aacccccact gcccgggcgc gtgggtgcgg tcggacagtt
     6481 gggccagcag cactccccgg tgcgagtgca cgaaggcgta tgccgcgagc cgctgcacgc
     6541 gctcggccac ctcccccgga gcctgcacca cgtccgggtc acgcgccggc acgcgcgccg
     6601 caggtgtccg gtccccgtcg gtgacttctc gaacgccgaa ggtcagaacg cagcggccct
     6661 cgcctgcctc agaccaaacg tcgagcaact catccatcac caaaccgttg tcccacagcg
     6721 tgattccagg gtcgttgcca tgctcaagca gccagggccc cacgacccga ccgtcggcca
     6781 ggcgcccttc cagccacagg tcccaggcgg tcatccggcg agcgcgcgcg cccgggcgta
     6841 ctcggcctcg gccgcctgca cgacctgatc ccagccctgc gccgggtcgg tcttgaggtt
     6901 gtatgccgtc atccgggacc gcacgctcgg gtggacggcc agcgtcgtca accgacgcac
     6961 caactcctcg tcgtcgttgg ccaggaagcc gttgacatcg tccgtgacga actccgagat
     7021 gcccgacccc acccgtccga cgaccggcag gccagcgacc cgagcctcca gcgccgcgat
     7081 gccgaaggac tcgaggaccg acggcgagac gaagacgtcg ctcgcggcat acctctcctt
     7141 gagggtgtcg cgcggcaccc ggccgggcaa ggacacccaa tccttcgcgc ccaaccggtc
     7201 gaccgtgcgc tcgaccttgg cgcgctcgga cccctcaccg aggatctcca ggcgcacgcc
     7261 tgctcgcggc gctgcggcgc gcaccttggc catcgcttcg acgagttgga cggggcgctt
     7321 ccgggcctcc aggcgcatcg ccgagacgaa ccgcacaacg ccgtcaccaa ggagtgggcg
     7381 gccgctggtg gggcgccacg cgtcgacgtc aataccgttg ggcagcaccg tgactggtgg
     7441 tccgtcgacg atcctgcgca gcggggtggc ggcgacgtcg gagacggcgt tcatcgccat
     7501 tcccgactgc gcccaacgcc ggacgatccc ggcagcgcgc acggccgact ccgccttgtc
     7561 caggacgcag tgccaggtca tcgtcgtcgg caggctcata cgactggtga cgaagccgca
     7621 gtccgacgcg aacggactga cgacccccat gtggatgtgg gcgacgtcga aaccctcgag
     7681 ttgggcccgc aaaccacggg tcgcaagcgg gttgacgagg agttggcgcg gcagccggac
     7741 cccgagccga tggacccgga tcccgtcgag cgtctcggtc tgaccgaagc cgaccgcgcc
     7801 cggcgtcaac gtgaagacct cgacctcgtg gccacgggcg accaaccgcg ccgacaggtc
     7861 ggcgacctgc acctcgatgc cgcccaggcg cgggaggtag cagtcggtga gcatggcgat
     7921 ccgcacggcc ctactctttc atgtgccgga gaatgggtct cgccatgcca ctgaccctgc
     7981 tcgccttcca cgcccacccc gacgacgagg ccatcctcac cggggggacc ctcgcgcgag
     8041 ccgccgccgc cggccaccgg gtcgtcctcg tcaccgccac cgacggcgct ctcggcctca
     8101 ccagcagcag gtatgccgcg caagggctgg cgagcgtccg cgcggccgag gtgcgcgaga
     8161 gcgcccggct gctcggggtg tcgcgggtcg agcagttggg gtatgccgac agcggcctgg
     8221 ggccggtgac tcttgacgac ccgccggggc aggtgcggtt tgtgcgggtg ccggtggccg
     8281 aggcggccga gcggctcgcg gccatcctgc gcgaggaatc ggtggacacg ctgctcacct
     8341 atgacgtcaa cggcgggtat ggccaccccg accacctgca ggtccaccgg gtcggtgcgg
     8401 ccgcagccca attggcccag acaccaaggg tattggaggt tgctgtctcg cagcgggtgg
     8461 ccaggctgat gaagccgcgc ggtctggcgt tggctcctgt ccgaccggtg acggtggagg
     8521 tcgacgtgcg ggagcagctg gacgccaaag ttgcggccat gcgggctcac gagtctcaac
     8581 tggcctccga cagcgcgctg ccgcgcaaca tcgacctgct gacccgactg ccccggcctg
     8641 ccctcgaacg gttgttgggc cgggagcggt tcgccgacct ctccgggtcc agcgaccccg
     8701 tgctttaggg cgactgcctg gttgagcgac cttcggtcta gtgaaccgcg gtgtccttga
     8761 gtttgccggt cccccgtgcc cgcagccggg tgatctgacg ggtgcgggcc tcgtcggcga
     8821 gggagtcgtc gacctgctca ggctcgaagg gccgaccggt ggcccgttcc gcagccgccg
     8881 cgatgagggc gtcggcgacc gcggggttgg cggggaggat cggtccgtgc aggtaggacc
     8941 cgatgacgtt gtgggtgcga gcgccctcgg tgtggtcctg gccgttgttg ccgtggccga
     9001 cccgaacggt gccgaagggg gcctggccgg agccgagcat cgttgcgccg gaatggttct
     9061 cgaagccgac gacctcgccc cactgggtgt ccaggacgat gccgccgatc atccgggtcg
     9121 actgcccgcg cgtcgtgacg tcgaggatgc ccaggccggg cagctcgaca ccgtcgctgg
     9181 tgatgaaggc gtgtccgaac atttggtaca tcccgcagat catcaccatc ggggtgccgt
     9241 cggcggccag gccacgcagc cggtcaccga tgacggccag gtcgtcctcg accctgctct
     9301 gaccggagtc ctgcccccca ccgcccatga gcaggtgcgc gtcctggggg aacggcatac
     9361 cggggtggtg gtcgtggacc tcgacgtcgt agccgtgacg acggatccgg gcggccaggc
     9421 atcgggtgtt gcccaggtcg ccgtagatcg acatctcgcg cgggtagagg tggacgagga
     9481 cgatccggcc cttgctggag gtgggcggca tactcactcg ccttcctgcg cgaagccggc
     9541 gagaccgaag cgcgcggcga ggagtttgcg cagcgccatc atcgccgtgt aggtgcagaa
     9601 gatgcgggtt ggctgcccgc ggtatgccgc gaggaagccg tcgagggctc ccgcgagatc
     9661 gggctcgatc tgctcgacaa gcacctcgtc gtagtccagt cgcagcgcca tgtcgtaggc
     9721 gcggaccccg ctcgtcatcg ccaccccgtg ctcgcgcagc gaggcgaacg acacgtcata
     9781 gagccaggac acgtcctgcc cgtcggcgta gtcgtcgttg atggcgatca tcgtggcgac
     9841 gggctcggcg ccataggtgc tcagcgcgac ggtgaaccca gcagggttct tgacgaggac
     9901 caactcgagc gggtggccgt cgacgtcgat gacctcgccc cgaccgaagg gcgctgtgac
     9961 ggtgcgcagg gccgcctcgg tgaggtcggt gcggaagtcg gcgccgagga cggcgcgggc
    10021 catcgtcgtg gcggcggtcg cgttgaccat ggcggcgagg ccgcgttggc gcagcgcgag
    10081 ggggccgacc tgctcggccg ggccgaagcg gatcgcgaag tcgcgggtgt tgacggtgtg
    10141 caggaggccg tcgtcggtgg tcgggggcgg cggggttgcg gcgtcctcga accgcacgtc
    10201 ggcctcgttg agcgcgggca cctggtcggc gatctcgggg tcgaccccga agtagcggat
    10261 ctccttgccc gacaagcctt ctgcgatgcg gaagacaaaa gagtcgtcgc ggttgaggac
    10321 gacgacctgg gaggtctggc cggccagggt cgccagcaac ttggcggtcg tgtcgatctc
    10381 ggcgaaccgg tcgagttggt cgcgggccac gttgagcagc agcgcgtggg tcggcgtgac
    10441 gagtttggcg aactgcagag cgtgccactc gtcgagctcg aggacggcga cgtcggcttg
    10501 caggcgcccg ccgacgccga tctccccgag catcgccgag aggaccccgc gggtgaagtt
    10561 gctgccggtg gggttggtga agacgtgctt gccatgcgcg gcgatgatgg cggccagcat
    10621 cttggtggtc gtcgtcttgc cgttggtgcc cgtgacgaca acgatcccgc ccggcacgct
    10681 ggccagggtg cgggccaaga tctgagggtc gacgcgctcg gcgaccaacc ccggcagggc
    10741 cgatccacca ccacgcaggc gcgcggcata ccggagtgtt tttccggcga tgatcgcggc
    10801 ggaggtccgg agcacaggcc aaccctaacc ggcctaccgg actggtcgag cgcgccacgg
    10861 agtgcgatca ctggggcgat caccccacca aacgcactcc gtgccgccca cgaggggtgc
    10921 gcggcatacc ctgtccgcgt gaccgacagc ccgtggcagc cgcagcaagg tcccttcggc
    10981 tatgtgcgtt tccacctgca gggctcggtc tggacgtcca gcctggtgat gccgaccgtg
    11041 accatcaacg gctatcgggt gcccgtcacg tacggcgaga acgtcattcc ggtctacccc
    11101 ggccggtgcg tcgtcgaggc gagcatgcgg tggatgcggc gctatgggca ggcgggatac
    11161 accgtggacg tcgcaccggg tcaggccgcg gacatctggt atgccgcgcc tttgacccag
    11221 ttccacaccg ggtcgatggg ggtggcgaag caatcccgca agggtggctg ggcgctcgcc
    11281 gggatgctgg cggcgatcat gctcatcttc ctggggctac ccctcacctt gcttttcgcc
    11341 ctcagccgtt agccggaccc gccggcctgt ggctgagacg gcgagcaccc ggcataccct
    11401 gtcgccgtga ccgatatccc gtggcagccg ttgccgccgg acccgctcgg gccgtgggat
    11461 gacctacccg cgcactcccc cgcccggccg ccggctgatt tccgtcccgc gccgcccgct
    11521 gagtccttcc accagccgct gccgccgaag gcgccggccg aggtggggta cctgcgcttc
    11581 catatgcagc gaacgtggtg gatcacgacg gggatcaagc cgatcctcac cctcaacggg
    11641 acgccggtga aggtgaagta cggcgagaac accatcccgc tgcagccggg gcgctatgtc
    11701 gtcgaagcga gccaggtgtg gagatcgcac cacggccacg cgagctaccc catcaccatc
    11761 gagccgggtg aggtcgtcga cgtctggtat gccggcccgg ccacccagta tcacaaagga
    11821 tcgatcggtc cgtccgagca gcagcgcgag ggcctacgga atgcgtacgt gttgttgttg
    11881 ggcgtcctcg gtgtcctcta tgcggccatg tttgcggtgg tgctcgtcac ctggaactga
    11941 cccccggcga cgcccgcctc gcagaccaaa cctcgccgat cgccaggcgc tactcccact
    12001 cgatggtgcc cgggggcttg cttgtcacgt cgaggaccac ccggttgacc tcgctcacct
    12061 cattggtgat ccgggtcgag atcttggcca gcacgtcgta aggaacccgc gtccagtcgg
    12121 ccgtcatggc gtcctccgac gacaccggcc ggagcacgat cgggtggcca taggtgcgcc
    12181 catcgccctg cacgccgacc gagcgcacct cagccaacaa cacgacgggg cactgccaga
    12241 tctcacgatc gagcccggcc gccgtcaact cctcgcgggc gatcgcatcg gccgctcgca
    12301 ggatcgccag tcgctcggcg gtcacctcac cgatgattcg cacgcccagc cccggcccgg
    12361 ggaacggctg gcgccagaca atcgcctcgg gcaccccgag ctccagaccg accgcccgca
    12421 cctcgtcctt gaacaacgcc cgcagcggct cgacgagggt gaacttcagg tccttgggca
    12481 gcccaccgac gttgtggtgc gacttgatgt tggccgcgcc ctcgcccccg ccggactcga
    12541 cgacatcggg atagagcgtg ccctgcacaa ggaactcgat ggggttcccg tcggccgacg
    12601 ccgagccgac gaggtcgcgc gccgcctgct cgaagacccg gatgaactgc gcgccgatcg
    12661 ccttgcgctt agcttccggg tcggtcaggc ccgccaacgc cgtgaggaag cgctcgcgcg
    12721 cgtcgacgac aaccaggcgc acgccggtcg ccgcgacgaa ctcctgctcc acctgctcgt
    12781 gctcgccctc gcgcagcagc ccgtggtcga cgaacacgca ggtcagttgg tcaccgacgg
    12841 cgcgctgcac cagcgccgcc gccacggacg agtcgacccc gccggacagc gcacacagca
    12901 cccgaccggt gccgacctgc gcccgcaccg actcgaccag cgactcaacg acgttggagg
    12961 ccgtccagtc cgcggggatc cccgcacctc gccacaggaa gttctccagc acgcgctgcc
    13021 cgtatgccgt gtgcagcacc tccgggtgcc actggactcc gaacaaccgc cggtccgcgt
    13081 cctcgaaggc cgccaccgtg gcccctgggg tcgtcgccgt cacggccatc ccggccggag
    13141 cctcggtcac cgagtcgccg tgcgacatcc acaccgactg ctccttcggc tgcccgtcga
    13201 agagcagcga accgtccggg gtcaccaccg cgtccgtgtg gccatactca cccgtccccg
    13261 tgtgggcgac cgtgccaccc aacgcctgga ccatcgcctg gaagccgtaa cacatcccga
    13321 acaccgggac cccggccgcc aacagggcgg ggtcaagcga cggtgcgccc gcggcataca
    13381 cactcgcggg gccgccggag aggatgaccg cagcgggatt cttggcgagc atggccgcca
    13441 ccgacatcg
//
LOCUS       DAOMZY010000045        13387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_4258_length_13387_cov_6.084608, whole genome shotgun sequence.
ACCESSION   DAOMZY010000045 DAOMZY010000000
VERSION     DAOMZY010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 13387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13387
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4258_length_13387_cov_6.084608"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PLK64_04205"
     CDS             complement(<1..195)
                     /locus_tag="PLK64_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78975.1"
                     /translation="MSTLDNLLRAAVAGVGGTERPGQVQMAYAVERAVQAREHLLVQA
                     GTGTGKSLAYLLPAVQHALDT"
     gene            305..1537
                     /locus_tag="PLK64_04210"
     CDS             305..1537
                     /locus_tag="PLK64_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV78976.1"
                     /translation="MSGFRERVLPRRLGPAYSLLLGSTWVSNIGDGIALAAGPLLMAS
                     VTRDPLLVAAAAVLQRLPWVLFGLYAGVLADRLDRKRMVVAVDVARAVVLLLFTVALT
                     GGTVHVWLVLAVMFLLGTAETFADTASPTLLPMIVDPPDLGIANARLMAGYLTMNQLI
                     APPIGAALFAIGVAVPFGTQAVCVAFGAVLVGRMKVPPMPPRAEVSHVRRDIAEGFRW
                     LWGNGPVRTLALTILVFNVTWGAAWSVLVLLAQDRLGMGPVGFGALTTAVAVGGLAST
                     AAYDWLERRFSLAALMRVCLLTETLVHLGLALTTMPAVALVVMFAFGAEAFVWGATAR
                     AVRQRAVPTEFQGRVGSVYTLGLFGGLVVGQALGGVLASLWGVTAPFWFAFGGSAILL
                     AIIWRELARIAHGTTTDA"
     gene            complement(1544..2941)
                     /locus_tag="PLK64_04215"
     CDS             complement(1544..2941)
                     /locus_tag="PLK64_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78977.1"
                     /translation="MPRRSGVGTGSRGGLGSLVLACIAGLLLIWLAPSPNAAADDSVL
                     PVIPSTAQVDAAKSAVATQGDRVAELDAAYQQATAEAGARQHAVATAAAAYAAAQAAE
                     DAAAHAAELAAAEADRAAQASAAARDAISRTAAAAYEQNGSTGELMLGLLTMEGPQRA
                     VDLAQYLEDSANSLDRQLRDAAMLAGVADSATAVAAERSRVLTEARLARAAALTGMEA
                     ELASATDALNAVAGRQRAMVAELAALQQTSEETQQQRLDGLAAAAAAQAAADAAAQRA
                     AREAAERAAAQAKPSPTPASRATPSTSNTPSTTTSTTSPPRPSASSSSSSSSTPKATS
                     TTTTSTTTPSASASPTSTPPPTSSTLAWAQAHPKTVAQQIMPTFGFDSSQWTCLESLW
                     QRESGWNWAADNPYSSAYGIPQALPGTKMATAGADWLVSPATQITWGLGYIKSRYGTP
                     CGAWSAWQSRSPHWY"
     gene            complement(3063..4157)
                     /locus_tag="PLK64_04220"
     CDS             complement(3063..4157)
                     /locus_tag="PLK64_04220"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPV78978.1"
                     /translation="MSLPALAELVADGVSVTQGSRVSIFTNDAAVLPAAAAFVEEVYR
                     RGGLAQIVHADEQFDASAVRWGSESMLRQPPPIEEAAMRWSDVHVSFRGLLPPTEALS
                     GARVAALREGRGRIATLRWEGTRWAIVRVPTAQWAEFAGVDAQTLMDEWSASFAEDWP
                     AAESRLMPWCAELAEASSVHIVGSDTDLVLGVGGRTWVPFAGRANWPDGEIATAPVED
                     QVDGHITFPGRFWFSGLEVADLRLNFAEGRVTSVAASAGEDFVRGLLATDDGAMRVGE
                     FGIGLNAALRTPTGDLLIDEKILGTVHIALGRSYQECGGRNRSALHWDIVKDLRDGGT
                     VRVGDRALIADGIVSDELRRYAVAAPDESR"
     gene            complement(4189..5697)
                     /locus_tag="PLK64_04225"
     CDS             complement(4189..5697)
                     /locus_tag="PLK64_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HPV78979.1"
                     /translation="MRSALGADRPLLGVDIGTFEAKGVLATADGTVLARARRRHGLLT
                     PAEGHVEHDPETVWWDGLCAVAAELMARPEARGGVEAVGVSGIGPCVLPVDAQLRPLR
                     SAILYGVDSRASRQIDSLTARLGPGEIFRRSGNSLSSQSAAPKIAWLRDEEPANWEAA
                     RWFLTCQSFLVARLTDEVVIDHATAGYFHPIYDIATQQWDLSDCDDIVEEARLPRLAW
                     SSEIAGTVTAAAHRATGIPEGTRVIVGTADAPAEAVAAGVIDQGSVMAMYGSSGFFIR
                     VGDSLVTDHTLWAAPFVFPGTFVLAAGVATSGTATRWIAEVLDIVGNTDQQTFAALIG
                     LAEQATPGCRGLLALPHFSGERTPFHDPQSRAAFVGLGLSHGRPELARGVIEGVAHAT
                     AAALAAYGKAGAPVRRVAAIGGGTKNDILLRSVSAIAGVTQTVVDSDGAALGDAALAA
                     LAVGAVDSRAQLAAWARIREVVDPDPALSDVLVPDHAKFLALYSALCQWRAR"
     gene            complement(5697..6500)
                     /locus_tag="PLK64_04230"
     CDS             complement(5697..6500)
                     /locus_tag="PLK64_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HPV78980.1"
                     /translation="MSWLSDLFCVTKPVIGMCHLSPLPGDPSYDRSRGVRAIVAHARR
                     EVEALQEGGVDGILISNEFSLPYLTKTEPITAVTMARIIGEINDAIEVPFGVNVLWDA
                     TASIDLAVATDAKFVREVFTGVYAGDFGLWNTNVGQTARHRLAVSGDDVRLLFNIVPE
                     AANYLAGRDLASVTRSTVFNCVPDALCVSGLTAGAPTDNSVLKVVKDNAGDVPVIVNT
                     GVRAETAEPSLRIADAAIVGTALKRDGIFENTVDVARVIELMDVVRSMR"
     gene            complement(6611..7660)
                     /locus_tag="PLK64_04235"
     CDS             complement(6611..7660)
                     /locus_tag="PLK64_04235"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV78981.1"
                     /translation="MMNRIAEADGMRRGGAERRDVLTDFVRKIHVNRSVGQMAALLVA
                     AFAIFTILRPAVFLGSYNLQVIALAAPEIGILAIAMMVTMLSGGIDLSVVSIANASAI
                     TISMTYTAVSSRAGVELANSLALPLVLLGLVVGVAAGAINGLLIAKVGITPILATLGT
                     MQLFNGLELVVTGGRTLYGAPAGLSTLGTTTFLGVPAIFLILVVVAVAIGVLLDRISL
                     GFRIRYQGANAEASRFSGISSTRTLLATYTIVGLLGGIAGVVFLSRNPSASADYGASY
                     TLLVIVIAVLGGTNPYGGRATVLGVVLATLCLQVVASGFTAMRLSSQQYAMAQGVILI
                     GAMVLDYLRGRGSSR"
     gene            complement(7657..8718)
                     /locus_tag="PLK64_04240"
     CDS             complement(7657..8718)
                     /locus_tag="PLK64_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007542173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV78982.1"
                     /translation="MSTPAQRQRRSGLLSRWPGRNNEGYLAALLLLVVAVMSAAAPAF
                     FSLHTFFSVIRGATVPWIFAMGVLLVLVSGGIDVSFPAIAVFSAYSAVALTTARGVDA
                     GLLGNFALAMAIGCLLGLVNGILIARFRLPTLIVTLGTAGFFRGAMITFVGATYIAQL
                     PRSLAEQSTRSVISVNTEAGTTFLHVLVVPVLILTVLIALGLKHTMFGRAAYAIGGDP
                     EAARRVGIPVQRTQVVLYVLVGALAAFGGMVFMIQGQAADPQMLIGIELDTIAAVVLG
                     GASIFGGRGSVLGTVLGVLLVQVINNSLVLVGVPTAWQRATVGLLLLLGVGAQAWAGR
                     RPRRAAPAAVKAPAVKAAA"
     gene            complement(8715..10220)
                     /locus_tag="PLK64_04245"
     CDS             complement(8715..10220)
                     /locus_tag="PLK64_04245"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007542174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPV78983.1"
                     /translation="MDISPAPRPVLELRDIVKTYGGVHALDTVSFTLLPGEVHCLAGE
                     NGSGKSTLIKIISGVETPDSGVIVVGGERHASMTPASAIAAGVQVIYQDFSLFPNLSV
                     AENIAMPRQRAVGGRFYSARAARPIAQGIVDELGLALDLDADVAALSVADKQLTAICR
                     ALVSDAQIIIMDEPTTALTGAEVTALFALVRRLQSRGVSLIFVSHKLDEVLRVSQRLT
                     VLRNGTWVATGAAGDYDRDAIAGLMTGKDIDTSRVVSAVPADAAIALEVKGLGRPGAF
                     ADVDLVVRCGEIVGLTGLLGSGRGEIAEAIFGVHPATTGTIAIGGHPVRIQSIPDAVA
                     AGIGYVPEDRLTQGLFLDDSIADNVVAASVTAFAGRARLLDRARMLAFIQTVASRLRI
                     KAPNLSLAVRSLSGGNAQRVVLAKWLMTNPKVLLLNGPTVGVDVGSKSEILEILRTQA
                     SAGMGVVVISDDIPELYSACHRVLIVQGGRIVCELAGDAVRVETIEQRIAA"
     gene            complement(10350..11399)
                     /locus_tag="PLK64_04250"
     CDS             complement(10350..11399)
                     /locus_tag="PLK64_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009212863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPV78984.1"
                     /translation="MTLTRLAKPAALLAVGSIALTAGCGSTAGTTSTSPSAAAKPVDA
                     KSMTMVTVVKLTGVGWFDRMNQGIKAFGQRTGVDARMDGADTASPEKQIAIIQNLIAQ
                     KPTAITVVPNSPAATESVLKSAREQGIVVVTHEAPGMTNVDADLEAFDNADYGAAIMD
                     AMASCMGKSGKYVQFVGALTAETHLAWVKGAYEKGKSAYPGMTRISDPIESKEDENTA
                     YARAKELLTRDPEIKGFQGSAATDIAGIARAVQETGRAADTCVIGTSIPSIAGKYLTD
                     KSIDQIFFWDPAAAGEAQLALALKIAKGEKITEGTDLGVDGYHKLKKSPTMANVWLGD
                     AQVSVDASTASKYPF"
     gene            11627..12391
                     /locus_tag="PLK64_04255"
     CDS             11627..12391
                     /locus_tag="PLK64_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007542176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HPV78985.1"
                     /translation="MVRKADILDDLLENGYRSVPDLALAFGVDASTIRRHLARLEKLN
                     LVQRTHGGALPIRHADTPFAVKQSLHTSEKVAIGAAMAARIEDGQTVLLDSGSTTLEV
                     AKRLTARGLTIVTNDLRIGLEVANRQLGHLVMLGGELLPSVYTLHGPATLRHLAEIRV
                     SVAVFSADSVMADGVFNSNAHEVELKRKMRDISSRSFLVADSSKFCRESLYKVFDLDN
                     FDAVLTDTGISPKVAGWFTIPVERHDARPAVTVPHL"
     gene            complement(12462..>13387)
                     /gene="hflX"
                     /locus_tag="PLK64_04260"
     CDS             complement(12462..>13387)
                     /gene="hflX"
                     /locus_tag="PLK64_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPV78986.1"
                     /translation="REGKAQVELAQLQYLLPRLRGWGESMSRQAGGRVAGGEGIGSRG
                     PGETKIELDRRRINTRIAKLKRDIAAMKTGRDTKRHSRRAGHVPSVAIAGYTNAGKSS
                     ILNRITGAGVLVENQLFATLDPTVRRAQTSDGREYTLVDTVGFVRALPHQLVEAFRST
                     LEEVADADLLLHVVDGSHPDPEGQIAAVREVLAGVKGASDVPEVIVINKADVADPEVI
                     DRLIRTHKHSIVVSARTGEGLDGLLELVADGLPKPDVEVELLLPYDRGDLLSRVHEHG
                     EVLTTEHTEAGTRVRAKVHAQAVAALAAYRV"
BASE COUNT         2069 a         4532 c         4655 g         2131 t
ORIGIN      
        1 ggtgtcgagg gcgtgctgga ccgcgggcaa gaggtaggcc agcgacttgc cggtgccggt
       61 cccggcctgg acgagcaggt gctcgcgggc ctggacggcg cgctccacgg catacgccat
      121 ctgcacctgg cccggccgct cggtgccccc gactccggcc accgcagcgc gcagcaggtt
      181 gtcgagggtg gacaccgcgc cactgtatgc cgtgccgccc gccactcgcg gcagccgtcc
      241 ccggccgggc cacgtggcaa accccacgcg gtgcggccgc cgagacgggt agcgtcatcg
      301 gtttgtgagc ggctttcggg agcgggtgtt accacgacgg ctcgggccgg cctactccct
      361 gctgctcggg tcgacgtggg tgagcaacat cggcgatggc atcgccctcg cggccggccc
      421 cttgctcatg gcgtcggtga cccgcgatcc tctgctcgtg gccgcggccg ccgtgctcca
      481 gcgactgccg tgggtgctgt tcggcctgta tgccggtgtg ctggctgatc gtctcgaccg
      541 caagcgcatg gtggtcgccg tcgacgtggc tcgggcggtc gtgctgctcc tcttcaccgt
      601 ggcgctcacc ggtgggacgg tgcacgtctg gctcgtcctg gccgtgatgt tcctgctcgg
      661 gacggccgag accttcgcgg acaccgcgtc accgacgctg ctaccgatga tcgtcgaccc
      721 acccgacctg ggcattgcca acgcccgcct catggcgggt tacctgacga tgaaccagct
      781 gatcgctccg ccgatcggag cggcgttgtt cgcgatcggc gtcgccgttc ccttcgggac
      841 ccaagccgtg tgcgtggcct ttggtgcggt gctcgtcggg cggatgaagg tgccgcctat
      901 gccgccgcgc gctgaggtca gccacgtgcg gcgggacatc gccgagggtt tccgttggct
      961 ctggggtaac gggccggtcc gaaccctggc cctcacgatc cttgtcttca acgtgacgtg
     1021 gggcgccgcg tggtcggtcc tcgttcttct ggcgcaggac cgactcggca tggggccggt
     1081 gggattcggt gccctgacga cggccgtggc ggtcggcggg ttggcgagca ccgcggcata
     1141 cgactggttg gagcggcggt tctccctcgc tgccctcatg cgggtgtgcc tgctcaccga
     1201 gaccctggtc catctcgggc tggccctgac gacgatgccg gcagtggcgc tggtggtgat
     1261 gttcgccttc ggtgcggagg cgttcgtctg gggcgcgacg gctcgggcgg tgcgccagcg
     1321 ggcggttccc acggagttcc aggggcgggt cggaagcgtc tacaccctcg ggctgtttgg
     1381 cgggttagtc gtggggcagg ccctcggcgg cgtcctcgcc tcactgtggg gcgtgacggc
     1441 gccgttctgg ttcgccttcg gcgggtcggc gatcctgctc gccatcatct ggcgcgagtt
     1501 ggcccgaatc gcgcacggca cgacgaccga cgcctgacgc cgatcagtac caatgcggag
     1561 aacgagattg ccaggcgctc caggcaccgc agggagttcc gtaccgactc ttgatgtacc
     1621 ccaaccccca ggtgatctgg gtcgccgggc tgaccaacca gtcggcgccc gcggtggcca
     1681 tcttcgtgcc gggcaacgcc tgcgggatcc cgtaggcgct cgaatagggg ttgtccgccg
     1741 cccagttcca gccgctctcg cgctgccaca gcgactcgag acacgtccat tggctggagt
     1801 cgaagccgaa cgtcggcatg atctgctgcg ccacggtctt gggatgcgcc tgggcccacg
     1861 ccagggtcga ggacgtcggc ggcggcgtgc tcgtcgggga cgcggaggct gacggggtgg
     1921 tcgtcgacgt cgttgtggtc gaggtggcct tcggcgtgga ggacgacgag gacgacgagc
     1981 tcgccgacgg gcgcgggggc gaggtcgtcg aggtcgttgt cgacggcgtg ttcgacgtcg
     2041 acggcgtggc ccgcgaggcg ggagtcgggc tcggcttcgc ttgggctgca gcgcgttcgg
     2101 ccgcctcccg agcggcccgc tgcgcggctg cgtcagcggc tgcctgcgcc gcggcggctg
     2161 ccgccagccc gtccagccgc tgctgctggg tctcctcgga ggtctgctga agcgctgcta
     2221 gctcggccac catcgctcgc tgtcggccag cgacggcatt gagcgcgtcg gtcgccgaag
     2281 cgagttccgc ctccatgccg gtgagggcag cggcccgcgc cagtcgggcc tcggtcaaca
     2341 cgcggctgcg ctcagcggca acagcggtcg ccgagtcggc gaccccggcg agcatcgcgg
     2401 catcgcgcag ttgacggtcc aggctgttgg cggagtcctc gaggtattgc gcaaggtcca
     2461 ccgctcgttg tggcccctcc atggtgagga gtcccagcat gagttctccg gtcgacccgt
     2521 tctgctcgta tgccgcggcg gccgttcggc tgatcgcatc gcgcgccgcc gcggaggcct
     2581 gcgcggcccg atctgcctct gccgcagcca gttcggcggc gtgagccgca gcgtcttcgg
     2641 cggcctgtgc ggcggcatac gcagccgcgg cggtcgccac agcgtgctgg cgcgcccctg
     2701 cctcggccgt tgcctgttgg tatgccgcgt cgagctcggc gacgcgatcg ccctgggtgg
     2761 ccacggccga cttcgccgcg tcgacctggg cggtgctggg gatgaccggc aggacggagt
     2821 cgtcggctgc ggcattcggc gagggtgcca gccagatcag caacaagcct gcgatgcagg
     2881 cgagcacgag cgacccgagg ccgcctcgtg acccggtgcc gacgcctgag cgcctaggca
     2941 tccgtgccag catgcgacct ccatcgttgg gacaacggcc ccacgatagc gagatctcac
     3001 cgattgcacc ataagaccca aggacccctc tgaccccgca cgacacgccg accgggcgtc
     3061 atttaccgag attcgtcggg cgcggccacg gcatacctgc gcagttcgtc cgagacgatg
     3121 ccgtccgcga tgagcgctcg gtcccccacg cgcacggtcc ccccgtcccg gaggtccttg
     3181 acgatgtccc agtggagcgc ggaccggttg cggcccccgc actcctggta ggagcgcccc
     3241 agggcgatgt gcaccgtccc gaggatcttc tcgtcgataa gcaggtcgcc cgtgggggtg
     3301 cgcagggcgg cattgagccc aatcccgaac tcccccaccc gcatcgcgcc gtcgtcggtt
     3361 gcgagcaggc ccctgacgaa gtcctccccc gctgacgcag ccacggaggt gacgcggcct
     3421 tcagcgaagt tcaagcgaag gtcggcgacc tccaagccgc tgaaccagaa tcgaccgggg
     3481 aaagtgatgt gcccgtcgac ctgatcctcc accggcgcgg tggcgatctc gccgtccggc
     3541 cagttggccc ggcccgcaaa cgggacccag gtgcgccctc cgacgccaag cacgagatcg
     3601 gtatccgacc cgacgatgtg aacgctcgac gcctcggcca gttcggcaca ccacggcatc
     3661 agccgggact cggcggctgg ccagtcctca gcgaacgacg cgctccactc atccatcagc
     3721 gtctgggcat cgacacccgc gaactcggcc cactgcgccg tcgggacccg cacgatcgcc
     3781 caacgggtgc cctcccaacg cagcgtggcg atccggcctc ggccttcacg cagcgcggcc
     3841 acccgcgcgc ccgagagcgc ttcggtcggg ggtagcaagc ctcggaagga cacgtggacg
     3901 tccgaccagc gcatggccgc ttcctcgatc ggcggcggct ggcgcagcat cgactcgctg
     3961 ccccatcgga cggcggaggc gtcgaactgc tcgtcggcgt ggacgatctg ggccagccca
     4021 ccccggcggt agacctcttc gacgaacgcc gcggcggcag ggagcacagc ggcatcgttg
     4081 gtgaagatgg agacccgaga cccctgggtc accgacacgc cgtcggcaac gagttccgct
     4141 agggccggga ggctcatgga cacggggacc gcctttcgca caacgagatc agcgagcacg
     4201 ccactggcac agcgcgctgt agagagcaag gaacttggcg tggtccggca cgaggacgtc
     4261 gctcagcgcc ggatccgggt cgacgacctc gcggatgcgg gcccacgcgg ccaactgcgc
     4321 gcgggagtcg accgcgccga ccgcgagcgc agccagggcg gcgtcgccca gcgcggcgcc
     4381 atcggagtcg acgaccgtct gggtcacgcc tgcgatcgcc gacacgctgc gcaggaggat
     4441 gtcgttcttg gtcccgccac cgatggccgc cacccggcgc acgggcgctc cggccttccc
     4501 gtatgccgcc agcgcggcgg cggtcgcatg ggccactccc tcgatgactc cgcgggcgag
     4561 ctccggccgg ccgtgggaga gcccgaggcc gacgaaggcc gctcggctct gagggtcgtg
     4621 gaagggcgtg cgttcgccgc tgaagtgcgg aagggccagc aggccacgac agccgggagt
     4681 ggcctgttcg gcaagaccaa tcagagcggc gaaggtctgt tggtcagtgt tgccgacaat
     4741 gtcgaggacc tcggcgatcc accgagtggc ggttcccgag gtggccacgc cggcggcgag
     4801 gacgaaggtc cccgggaaga cgaagggcgc agcccacagg gtgtggtcgg tcacgaggga
     4861 gtcgccgacc cggatgaaga agcccgacga tccatacatg gccatcaccg aaccctggtc
     4921 gatgacaccg gcggccacgg cttcggcagg ggcgtcggcc gtcccgacga tgacgcgggt
     4981 gccttccggg atgccggtgg cacggtgcgc agccgcagtc acggtccccg cgatctcgct
     5041 cgaccaggcc aaccggggca accgggcttc ctcgacgatg tcatcgcagt cgctcaggtc
     5101 ccactgctgc gtggcgatgt cgtagatcgg gtggaagtag cccgccgtgg catggtcgat
     5161 gaccacctcg tcggtcagtc gggccacgag gaaggactgg caggtgagga accaccgcgc
     5221 tgcctcccag ttggcgggct cctcgtctcg cagccaggcg atcttggggg ctgccgactg
     5281 actggacagc gagttgccag agcgtcggaa gatctccccc ggccccagcc gcgcggtgag
     5341 tgagtcgatc tggcggctgg cccgcgagtc gacgccgtag aggatcgccg agcggagcgg
     5401 ccggagttgc gcatcgacgg gcaggacgca cggtccgatc ccgctcactc cgaccgcttc
     5461 aacgccgccc cgggcctcgg gtctggccat caactccgcg gccacggcgc acagcccgtc
     5521 ccaccacacg gtttcggggt catgctcgac atggccctct gcgggggtga gcaggccgtg
     5581 tctgcggcgg gcccgagcca gcaccgtccc gtccgcagta gccagcacgc ccttggcctc
     5641 gaacgtcccg atgtcgaccc ccaggagcgg gcggtcggca ccgagggcgg atcgcatcag
     5701 cgcatgctcc gcacgacgtc catcaactcg atgaccctgg ccacatccac agtgttctcg
     5761 aagatgccat cgcgcttcaa ggccgtcccg acgatcgcgg catcggcgat ccgcaggctc
     5821 ggctccgcgg tctcggcccg gactccggtg ttgacgataa ccggaacgtc gccagcgttg
     5881 tccttgacca ccttgaggac tgagttgtcc gtcggagcgc ctgccgtcag tccggagacg
     5941 cacagggcat cgggaacgca gttgaacacc gtcgagcggg tcaccgacgc gaggtcgcgc
     6001 cccgcaaggt agttggcggc ctcgggcacg atgttgaaga gcaaccggac gtcgtcgccg
     6061 gacactgcca accggtggcg cgcggtctgt ccgacgttcg tgttccacag accgaagtca
     6121 ccggcataca cccccgtgaa cacctcacgc acgaacttgg cgtcggtcgc gacggcgagg
     6181 tcgatgctgg cggtcgcgtc ccagaggacg ttcacgccga agggaacctc gatcgcgtcg
     6241 ttgatctcgc cgatgatgcg ggccatggtc acagccgtga tgggctcggt cttggtcagg
     6301 tagggcaggc tgaactcatt cgagatgagg atgccgtcca cgccaccctc ctgaagcgcc
     6361 tccacctcgc ggcgggcgtg ggcgacgatt gcccggacgc cgcgactcct gtcataggag
     6421 gggtccccgg ggagcgggga gaggtgacac atgccgatga ccggcttggt cacacagaag
     6481 aggtcgctga gccaggacac gctggtactg cctttcgaaa cgtagggggt ggtgctgggg
     6541 tggtggctgg gattgtggct ggggttggtt gtggggttga cgctggccga cactgacgcc
     6601 ggatcgggtc tcatctcgag gaaccacgac ctcggaggta atccaggacc atggcgccga
     6661 tgaggatgac gccctgcgcc atggcgtatt gctgggagct cagtcgcatg gccgtgaatc
     6721 cgctggcgac gacctgcagg cagagcgtgg ccagcaccac gccgaggacg gtggcccggc
     6781 cgccgtacgg gttggtgccg ccgagcacgg cgatgacgat gacgagcagg gtgtaggagg
     6841 cgccgtagtc ggcactcgcg ctggggttgc gcgacaggaa gaccacgccg gcgatgccgc
     6901 ccagcagtcc gacgatggtg taggtcgcca gcagagtccg cgtcgagctg atcccggaga
     6961 accggctcgc ctcggcgttg gctccctggt agcggatccg gaaacccagc gagatccggt
     7021 ccaggagcac accgatcgcc acggccacca cgacgagaat gaggaagatt gccggcactc
     7081 cgaggaacgt cgtcgtcccc agcgtgctca accccgccgg ggccccgtaa agcgtcctgc
     7141 cgccggtgac gacgagctcg agcccgttga acagttgcat cgtgcccagg gtggcgagga
     7201 tgggggtgat cccgaccttc gcgatgagca gtccgttgat cgcgcccgcc gccaccccga
     7261 ccacgagccc cagcaggacc aacggaagag cgagcgagtt ggccagctcg accccggccc
     7321 gagagctcac ggccgtgtag gtcatcgaga tcgtgatcgc cgaagcgttc gcgatggaga
     7381 cgaccgacag gtcgatcccg ccactgagca tcgtcaccat catggcgatg gcaaggatgc
     7441 cgatctcggg ggcggccagg gcgatgacct ggaggttgta cgagcccagg aacacggcag
     7501 ggcgcaggat ggtgaagatc gcgaaggcag ccacgaggag cgcggccatc tgtccgaccg
     7561 agcgattgac atggatcttg cggacgaagt cggtgagcac gtcccgacgc tcggcgccac
     7621 cacggcgcat gccgtccgcc tcggcgatgc ggttcatcat gccgctgcct tcacggccgg
     7681 ggccttcact gctgcgggcg ccgcgcgacg cggtctgcgc ccggcccacg cttgggcgcc
     7741 gactccgagc aggagcagca ggccaactgt ggctcgttgc caggcggtcg ggaccccgac
     7801 cagcacgagg ctgttgttga tcacctggac gagcaacaca ccgagtaccg tgccgaggac
     7861 cgagccgcgt ccgccgaaga tcgacgcccc tccgaggacg acggccgcga tggtgtccaa
     7921 ctcgatgccg atgagcatct gggggtcggc ggcctgcccc tggatcatga agaccatccc
     7981 gccgaaggcc gcgagggcgc cgaccagcac gtagaggacg acctgagtcc gctgcaccgg
     8041 gataccgacc cgccgggccg cctcagggtc accaccgatg gcgtatgccg cgcggccaaa
     8101 catcgtgtgt ttcaggccga gcgcgatgag caccgtgagg atgagcaccg ggacgacgag
     8161 gacgtgcagg aacgtcgtcc ccgcctccgt gttcacggag atcaccgagc gggtgctctg
     8221 ctcggccagc gaccgcggca gttgggcgat gtaggtggct ccgacgaagg tgatcatcgc
     8281 gccgcggaag aagcctgccg tgcccaatgt gacgatcagt gtcggcaggc ggaaccgcgc
     8341 gatgaggatg ccgttcacca gcccaaggag gcagccgatg gccatggcga gcgcgaagtt
     8401 tccgagcagt cccgcgtcga cgcctcgcgc cgtggtgagg gcgaccgcgg agtatgccga
     8461 gaacacggcg atcgccggga aggagacgtc gattccgccg gacaccagga ccagcaacac
     8521 gcccatggcg aagatccacg ggacggtcgc gccacggatc accgagaaga aggtgtgcag
     8581 gctgaagaac gccggtgcgg cagcggacat cacggccacg acgagcagca agagggcagc
     8641 gaggtagccc tcgttgttgc gacccggcca gcgggacagc aaccccgagc gcctttggcg
     8701 ctgtgccggc gtgctcatgc ggcgatccgc tgctcgatgg tctcgacccg gacggcatcc
     8761 ccggccaact cgcagacgat tcggcccccc tgcacgatga ggacccggtg gcacgcgctg
     8821 tacaactcgg ggatgtcgtc ggagatgacg acgacgccca tccccgcgct cgcctgggtc
     8881 cgcaggatct cgaggatctc ggacttggag cccacgtcga cgccgacggt ggggccgttg
     8941 agcagcagca ccttggggtt ggtcatcaac cacttggcga gcacgacacg ttgggcgttc
     9001 ccgcccgaga ggctgcgaac cgcgaggctg aggttgggcg ccttgatgcg tagccggctc
     9061 gcgacagtct ggatgaaggc gagcatccgc gcccggtcca gcagcctggc ccgaccggca
     9121 aatgcggtga ccgaggccgc cacgacgttg tcggcgatgg agtcgtcgag gaacaaccct
     9181 tgggtcagtc ggtcttccgg gacgtagccg atccctgcgg cgaccgcgtc cgggatcgat
     9241 tggatgcgca ccgggtgacc gccgatggcg atcgtcccgg tggtggccgg gtgcaccccg
     9301 aagatggcct cggcgatctc gccgcgcccc gaaccgagca gcccggtcag accgacgatc
     9361 tccccgcacc ggaccaccag gtcgacgtcg gcgaacgcgc ccggtcggcc gagacccttg
     9421 acctccaggg cgatggcggc gtcggccggg acggcagaca ccacccgcga agtgtcgatg
     9481 tccttgccgg tcatcagccc ggcgatcgca tcgcggtcgt agtcccccgc cgcgccggtg
     9541 gcgacccagg tgccgttgcg cagcacggtg aggcgttggg agacccggag cacctcgtcg
     9601 agtttgtggc tgacgaaaat cagcgacaca ccgcgggact gcaaccggcg aacgagggcg
     9661 aacagcgcgg tgacctcggc tccggtcaac gccgtggtgg gctcgtccat gatgatgatc
     9721 tgggcgtcgc tgacgagcgc ccggcagatg gcggtcaatt gcttgtcggc aaccgacagg
     9781 gccgcgacat cggcatccag atcaagggcc aggccgagct cgtcgacgat cccctgcgcg
     9841 atggggcggg ctgcgcgggc cgagtagaac cggccaccca cagcgcgttg gcgcggcatg
     9901 gcgatgttct cggccacgct gaggttcgga aagagcgaga agtcctggta gatgacttga
     9961 accccggctg cgatggcgct ggccggggtc atgctcgcgt gccgctcgcc gccgacgacg
    10021 atcacgccgg agtcgggagt ttccacgccg ctgatgatct tgatgagcgt cgacttcccg
    10081 ctgccgttct caccagcgag gcagtgcacc tcgccgggca ggagtgtgaa ggacacggtg
    10141 tcgagggcgt ggacaccacc gtaggtcttg acgatgtcgc ggagctccag cacgggccgt
    10201 ggggccggtg agatgtccat ggtcgtcctt ggttcggatg gagcagcggc ctcggtgttg
    10261 ggtcggggcc tccccggtca gaagcggtgg ggggaaaccg gagaggcccc gacggttgag
    10321 cggtctgcgc gcttgcgcag ccgcagctct tagaacgggt acttcgaggc ggtgctggcg
    10381 tcaacggaga cctgcgcgtc accgagccag acgttggcca tggtgggcga cttcttgagc
    10441 ttgtggtagc cgtcgacacc caggtctgtg ccctcggtga tcttctcccc cttggcgatc
    10501 ttcaaggcca gcgcgagctg cgcctcaccg gccgcggccg ggtcccagaa gaagatctgg
    10561 tcgatcgact tgtcggtcag atacttgccg gcgatggagg ggatgctcgt cccgatgaca
    10621 caggtgtcgg cggctcggcc cgtctcctgc accgccctgg cgatgcccgc gatgtccgtc
    10681 gcggctgacc cctggaaacc tttgatctcc gggtcgcggg tgagcagttc cttcgccctc
    10741 gcgtatgccg tgttctcgtc ttccttggat tcgatcgggt cgctgatcct ggtcataccg
    10801 gggtaggcgg acttgccctt ctcgtaggca cccttgaccc aggccaggtg ggtctcggcc
    10861 gtcagcgccc cgacgaactg gacgtacttc ccggacttgc ccatgcacga ggccatcgca
    10921 tccatgatgg cggcgccgta gtcggcgttg tcgaaggcct caaggtcggc atcgacgttg
    10981 gtcatgcccg gtgcctcgtg ggtgaccacg acgatgcctt gctcccgggc actcttgagg
    11041 accgattcgg tcgcggccgg cgagttcggg acgacggtga tggccgtcgg cttctgggcg
    11101 atgaggttct ggatgatcgc gatctgcttc tcggggctcg cggtatcggc accgtccatc
    11161 cgcgcgtcca ccccggtccg ctgaccgaag gccttgatgc cttgattcat tcggtcgaac
    11221 caacccacgc cggtgagctt gaccacggtg accatcgtca tcgacttcgc gtcgaccggc
    11281 ttcgccgcag cgctggggct cgtcgaggtg gtccctgcag tgctaccgca gccagcggtg
    11341 agcgcgatcg acccaacggc gaggagtgct gccggcttgg cgagcctggt aagcgtcatg
    11401 cgggttctcc atgtttctgt cgaaccgccg gggctggcgg ttgccatgac gtgaacgtta
    11461 cgcacgtgtt cgcgcagtgt caataccctt cttgggacaa tttgcccggt tacgttcgtc
    11521 ccagtgcctg atatcaggca ctcatctggc gctaggtggg cgaaacgcgc tacggtgggc
    11581 acctcttctc cacgagaagg cgcgtggcac accaccaagg agcctcatgg tccgcaaggc
    11641 cgacattctc gatgacctcc tcgagaacgg ctatcgcagc gtccccgatc ttgcgctggc
    11701 gttcggggtg gacgcgtcga ccatccgccg gcatctggcc cggctcgaga agctcaacct
    11761 cgtgcagcgc acgcatggcg gcgccctccc catccgccac gcggacactc cgttcgccgt
    11821 caagcagtcg ctgcacacct ccgagaaggt ggcgatcggc gcggccatgg ccgcccggat
    11881 cgaggacggc cagaccgtct tgctcgacag cggctccacg acgttggagg tcgccaaacg
    11941 cctcaccgcg cgcggcctga ccatcgtgac gaacgatctg cggatcggcc tcgaggtcgc
    12001 taaccgacaa ctcggccacc tagtcatgct cgggggcgag ctactcccca gcgtgtatac
    12061 gctgcatggg ccggcgacgc tgcgtcatct ggccgagatc cgcgtctcgg tcgccgtgtt
    12121 cagcgccgac tcggtgatgg ccgacggggt cttcaactcc aacgcccacg aggtcgagct
    12181 caaacgcaag atgcgcgata tctccagccg atccttcctc gtcgccgact cctccaagtt
    12241 ctgccgggag tccctctaca aggtgttcga cctggacaac ttcgacgccg tcctcaccga
    12301 caccgggatc agcccgaagg tcgccggctg gttcacgatc cctgtcgagc gccacgacgc
    12361 gcggcccgcg gtcaccgtcc cgcacctctg agccatagct ccttcacccc gccgacgtga
    12421 cacacgtcgg gggcgccgcg cggcataccc acgtggcagg atcagacccg gtatgccgcc
    12481 agcgcggcca ctgcctgcgc gtgcactttt gcacgaactc gcgtacctgc ttccgtgtgc
    12541 tcagtggtga gcacttcccc gtgttcgtgc acccggctga gcagatcacc gcggtcgtag
    12601 ggcagcagca actcgacctc gacgtcgggc ttgggcagcc cgtcggcgac gagttccagc
    12661 aggccatcaa gcccttcgcc ggttcgggcc gacacgacga tcgaatgctt gtgggtgcgg
    12721 atcaaccggt cgatgacctc gggatcggcg acgtcggcct tgttgatgac gatgacttca
    12781 ggcacgtcgc tggcaccttt gacgcccgcg agcacctcgc gcacggcggc gatctgaccc
    12841 tcggggtccg ggtgtgaccc gtctacgacg tggagcagca gatcggcgtc ggcgacctcc
    12901 tcgagggtgg agcggaacgc ctcgacgagt tggtgcggca gcgcccgaac gaatccgacc
    12961 gtgtcgacga gcgtgtactc ccggccgtca gacgtctggg cccgccggac cgtcgggtcg
    13021 agggtcgcga acaactggtt ctcgacgagc acgccggcac cggtgatccg gttgaggatc
    13081 gaggacttac cggcgttggt gtagccggcg atcgccacgc tcggcacatg cccggcccgc
    13141 cgcgagtgcc gcttggtgtc gcggccggtc ttcatggcag cgatgtcccg cttgagcttg
    13201 gcgatccgcg tgttgatccg gcgtcgatcc agctcgatct tcgtctcacc cggcccacgc
    13261 gacccgatgc cctcgccacc ggcgacccgc ccaccggcct gccggctcat cgactcaccc
    13321 cacccacgca ggcggggcag gaggtactgc agttgggcga gctcgacctg ggccttgccc
    13381 tccctgc
//
LOCUS       DAOMZY010000046        13379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_4261_length_13379_cov_6.306515, whole genome shotgun sequence.
ACCESSION   DAOMZY010000046 DAOMZY010000000
VERSION     DAOMZY010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 13379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13379
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4261_length_13379_cov_6.306515"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..658)
                     /locus_tag="PLK64_04265"
     CDS             complement(101..658)
                     /locus_tag="PLK64_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV78987.1"
                     /translation="MSRIPVSRRPGARLLVALATLMVVNGCASSTPGLVANSASYLAQ
                     GSGDGRITFGIQICAPPGVNVWVTDIQPLAWVGSGFDVLGFRLASYDAPGGITVATSG
                     FPPAIPTRPGEQVYAVGDHVAVQPCESAQRLSEVQVGLARRGPSGGGLSSVRITYGGT
                     VSGEIVTYVGMLFCGPSTTDCPVDR"
     gene            801..1994
                     /locus_tag="PLK64_04270"
     CDS             801..1994
                     /locus_tag="PLK64_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HPV78988.1"
                     /translation="MTTAPAFGPLAGVKIIELAGIGPGPFAGMVLADLGADVIGVDRP
                     GGAGPFDLPVLRRGRRSIAVDLKSEAGREVILDLVASADALIEPYRPGVMERLGLGPD
                     AVLARNPRLVYARMTGWGQDGPQSPYAGHDLTYTATSGILSSFVRDPHDGSMPRPVLP
                     GPYLGDVAGGALLLVAGLLAALHSAAATGTGQVIDAAIMDGASYLSTFTHALRLGGMW
                     GGTPGNNILDTGAPFYEVYECADGRHLAVGALEPQFYDELLRLLAEHAGLDLGPDDPR
                     HRLNRLDQRRWPEAKALWEDFFRTRTRDEWCAFLERSDACVAPVLSLEEAPTHPHLGA
                     RAAYLPVGGDHGPELPVPAPRFSKTPTQTRPSPARGAHTDELLAEVGYHPARIATLRS
                     TSVVA"
     gene            2082..3956
                     /locus_tag="PLK64_04275"
     CDS             2082..3956
                     /locus_tag="PLK64_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HPV78989.1"
                     /translation="MSKPVQQLDRVVIRFAGDSGDGMQLTGDRFTSDAAFHGNDLSTL
                     PNFPAEIRAPQGTIHGVSSFQLHIADHDVLTPGDAPDVLVAMNPAALKANLADLPRGA
                     TIIVDTDEFSRRNLAKVGYAVSPLDDGSLESYHVHALPLTSITIDALAEHPLTRKDKE
                     RAKNMFALGLLSWLYTRPTSTTERFILSKFARKPDLLAANVAALRAGFAFGETTEDFA
                     HTYQVAAASMPDGTYRNITGNVALSYGLVAAAHQAGLRLVLGSYPITPASDILHTLSG
                     LKRFDVTTMQAEDEIAGVGIAIGASFGGALGVTTTSGPGLALKAEAIGLAVSLELPLV
                     VVNVQRGGPSTGLPTKTEQADLLQAMFGRNGESPVAIVAPQSPTDCFDAAIEACRIAT
                     AYRTPVILLSDGYLANGSEPWKVPVPAELPALDVTFTTEPNGVDASGSPVFQPYLRDP
                     ETLARPWAIPGTPGLEHRIGGIEKADVTGNISYDPDNHELMTKLRAGKIAKVADTIPG
                     LEVDDPDGDARVLVIGWGSTYGPIRQAARSARLTGHRVATTHLRHLNPFPLNTGEVLG
                     RYERVIMPEMNLGQCAMLLRAAYLVDVQSHTAVRGLPFKASELAEIIRTAVDGLEATK
                     "
     gene            3953..5032
                     /locus_tag="PLK64_04280"
     CDS             3953..5032
                     /locus_tag="PLK64_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HPV78990.1"
                     /translation="MSIDLGMPSSGVAFVPQLAEGSPALGRKDYTSDQDVRWCPGCGD
                     YAILAAMQGFLPELGLRKENIVFISGIGCSSRFPYYLDTYGLHSIHGRAPTFATGLAT
                     AREDLSVWVITGDGDALSIGGNHLIHALRRNVNIKILLFNNEIYGLTKGQYSPTSRAG
                     TITKSSPLGSLDAPFTPVSVALGAEATFVARTMDSERKHLTEVLHAAAAHRGAALVEI
                     YQNCPIFNDGAFDVLKDKDEAAARILWLKDGEEVATGDRVAVRADDGSIQVVPRAEAS
                     PTRIVRHSVDTEHPSSAFALSRLTSDGLLAHVPMGVFRDVSRPTYDDMVRGQVSAAVE
                     AAGGPATDADLAALLAGRDTWTVGG"
     gene            5029..5934
                     /gene="rarD"
                     /locus_tag="PLK64_04285"
     CDS             5029..5934
                     /gene="rarD"
                     /locus_tag="PLK64_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HPV78991.1"
                     /translation="MTPALNPGSEVRRGALFGTAAYMLWGFFPLYFHGLSPATPLEVL
                     AHRILWSLFFCLIILAWRRDVTWVRPLFARPRLLAGLTAAALLVAINWGVYVAAVLSG
                     HTRDAALGYFLNPLITVALGVVLLREPLRRLQWMAVAIGLVAAVFLTVAGGQVPVIAL
                     TLAVTFALYGLLKKRIGASLGALHGLTIETAVLLPIALLLLGPLSGLGGTHAGFGSVG
                     QAAGLILTGPITAIPLLLFAAAARRVPLVTIGLLQFLTPVMQLLCGLLLGEVVTPTQW
                     VGFVIVWVALGVLTVDSLRQVARSR"
     gene            5953..6423
                     /locus_tag="PLK64_04290"
     CDS             5953..6423
                     /locus_tag="PLK64_04290"
                     /inference="COORDINATES: protein motif:HMM:NF022636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HPV78992.1"
                     /translation="MTITRLLRVLLVAFAAGLAVGVLMAHWLSLDVAQTYPEVAHLRA
                     PAFWAAVVGLVPLVVAAGAMWRFLALVDRGEAFSGASARLVRRVSVVFLVLAVYIAVA
                     AVVLGGALPQSSPTFTLAVMAAESVALFLMAACALLGRLLREAGLPLHRNPLPA"
     gene            complement(6550..7548)
                     /locus_tag="PLK64_04295"
     CDS             complement(6550..7548)
                     /locus_tag="PLK64_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPV78993.1"
                     /translation="MSAPQVLSLPGVSPELAAALADGMGRVDALLRSTVDHDDPYVAA
                     ASAHLLAAGGKRFRPTLTLLAAQVCRGVTDEVIAAAVGVELTHLASLYHDDVMDEAAV
                     RRAVPSVNAAYDNTTAILVGDLLFGKASEIVAGLGAEAVRIQAQTFVRLCFGQIRDTR
                     ACPPDVDPIDYYLGVLADKTGALVATAARYGAMFGGGSPAMVEVLRVYGERLGMAFQL
                     ADDIIDIASDGAMSGKTPGTDLREGVDTLAVLFAKASSHPADARLQELLAADLTDDVL
                     HAEALALLRANPALERARAHTREVGAQAREALAGLPDSDAKAALVALVDQVVERVG"
     gene            complement(7583..9172)
                     /gene="nuoN"
                     /locus_tag="PLK64_04300"
     CDS             complement(7583..9172)
                     /gene="nuoN"
                     /locus_tag="PLK64_04300"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HPV78994.1"
                     /translation="MTTALSAAFTPATLDYVALLPVLIIFSAAMLGVLVEAFVPRTSR
                     YLAQVTITLVGIVAAMAALAFGAVTHRVSTAGIAGFNGTVKGSVVIDGPALFIQATIL
                     LFALLAALSLAERFGGVGADAFTPMGAATPGSPQEAQARKVGAMTAEVFPLFLFSVAG
                     MMLFPAAGDLLTMFVALEVLSLPLYLLCGLARRRRLLSQEASLKYFLLGAFSSAFFLF
                     GTALLYGFAGSVYLPDISAAITAQGSAFQGLLVPGVLLVLVGLLFKVGAVPFHMWTPD
                     VYQGAPTPITGFMAAGTKAAAFGAILRFVYVGVDRAQWDWRPAVVVVAALTMIIGAVL
                     SVTQTDMKRLLAYSSIAHAGFILVGVLSMERDGVSGVMFYIAAYGLTTIAAFAIVGLV
                     REGGSEATHLSQWTGLGKRHPWTAGVFAFLLLAFAGIPLTSGFTAKFAVFKPALESGT
                     AGTILVVLGLLCSAITVFVYVRIIVLMYFTDSTDDSVAVIEPSVFTTVTVATGTLFTL
                     VLGIAPASVLQLAERASQFIR"
     gene            complement(9169..10704)
                     /locus_tag="PLK64_04305"
     CDS             complement(9169..10704)
                     /locus_tag="PLK64_04305"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HPV78995.1"
                     /translation="MNQFPWLTVIGLIPLVGAVVLWLLPASLADRAKHIALGASLVTL
                     VFAVAAALQFSTSSTSKFQLVEEHSWIPQFGVSYALGVDGIALSLILMSVILVPVCLL
                     AAWNDLPAEEAHRERHYFALMLVLETFMVGVFAATDVFLFYVFFEAMLVPVYFLIGQF
                     GGPRRQYAAVKFLLFSLAGGLIMLAAVIGLYVQGPGGADGFLLSKLAGIQLAPGTEKW
                     LFLGFFIAFAVKAPMVPVHTWLPDAAAESRPATATLLVGVLDKVGTYGMIRFCLELFP
                     GASQWATPVVITLALVSILYGALLAIGQTDLMRLIAFTSISHFGFIVLGIFAMTSTSW
                     AGSSFYMINHGFSTAGLFLVAGMFISRRGSKRIPDFGGWQRVTPALAGVWLLVGLSGL
                     ALPGLGSFISEFLVLAGTFQRYQVVGVIATTSIILAALYILLMYQRTMTGPKPESAAG
                     TPDISYREKWVVGPLIALFLILGFYPKPVLDMVTPAVTTILTSIHVTDPAPAVGAAEG
                     STK"
     gene            complement(10704..12626)
                     /gene="nuoL"
                     /locus_tag="PLK64_04310"
     CDS             complement(10704..12626)
                     /gene="nuoL"
                     /locus_tag="PLK64_04310"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HPV78996.1"
                     /translation="MPFAGEASTVHAATGFASAAWLLVALPLLGAAVLLLGGHRTDKW
                     GPLFATAMSWAAFVVGLGVFITLLGLSSEERALTVHLFNWVPAGQFQLEAGMLLDPLS
                     IAFVLLVTFVGSLIHVYSLGYMEHDPDKRRFFAYLNLFVAAMLLLVLANSYLLVYVGW
                     EGVGLASYLLIGFWNWNPPYASAANKAFVANRVGDVGMGLAIMAMFWQFGAVDFQTVN
                     GAVAEANEGALTVIGLFLLLAACGKSAQFPLQSWLGDAMAGPTPVSALIHAATMVTAG
                     VYLVVRSHAIFDGAPNAQLAVVIVGAITLVYGAIVGCAKDDIKKALAASTMSQIGYMM
                     LAAGLGPVGYAFAIFHLLTHGFFKAGMFLGAGSVMHGMNDKVDMRGFGNLSSVMKITW
                     VTFGLGWLAILGVPPFAGFWSKDKIIEAAFVGEGWRPWVFGLAALIGAGITAFYMSRL
                     FFMTFQGEKRWPKDAHPHESPRTMTIPMMVLAVGSASLGLILGPTNGILHWLEPVVGA
                     AEEEHPVIPVAALMAITLLVVAAGVALAWMRYWRDKVPTTAPVGSLLTRAARADLYQD
                     QVNEGVFMRPGIHLTRALVFLDKRGIDGAAGGFAALIGGTSSRLRRVQNGLARSYALT
                     MLIGVVVILGAVWVVQ"
     gene            complement(12680..12979)
                     /gene="nuoK"
                     /locus_tag="PLK64_04315"
     CDS             complement(12680..12979)
                     /gene="nuoK"
                     /locus_tag="PLK64_04315"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HPV78997.1"
                     /translation="MNLINYIYLSILLFTIGAATVLVRRNAIIIFMGIELMLNAANLA
                     FVTFSRIHGTLDGQVIALFVMVVAAAEVVVGLAIIMAIFRARRSASVDDANLLKL"
     gene            complement(12976..>13379)
                     /locus_tag="PLK64_04320"
     CDS             complement(12976..>13379)
                     /locus_tag="PLK64_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HPV78998.1"
                     /translation="AGLDKVNVDPGNVSGLADLIFGKYVWVFEITSALLITAAVGAMV
                     LAHRERLTPKSTQRELAARRVKDNVFVAGLPAPGVYARSNSVDTPALLPDGTPSELSV
                     SRVLIAREQIVAPDGTSTAHASAEIEEGRSR"
BASE COUNT         2257 a         4590 c         4568 g         1964 t
ORIGIN      
        1 gcgggtggag ggacctgagg cgttggccgc gcaaagcggc caacgcctca gtgttccttc
       61 gtgatcttct cccgagcccg cggtcgatca gagatccctg ctaccggtcc accgggcagt
      121 cggttgtcga cgggccgcag aagagcatgc cgacgtaggt cacgatctct ccggagacag
      181 tgccgccgta agtgattcgc acgctcgaca gaccgccgcc cgaaggaccg cgacgagcca
      241 gaccgacttg gacctcgctc agtcgctgcg cactctcgca cggctgcacc gcgacgtgat
      301 cgccgacggc atacacctgc tcccccgggc gcgtcgggat agcaggcggg aaccccgagg
      361 tcgccacagt gatacctccg ggcgcgtcgt aactggccaa ccggaagccg aggacgtcga
      421 agccgctgcc gacccacgcc agcggttgga tatcggtgac ccacacgttc acccccggcg
      481 gcgcacagat ctgaatcccg aaggtgatcc ggccgtctcc actgccctgg gcgaggtaac
      541 tggccgagtt tgccaccagc ccgggggttg acgatgcgca cccgttgacg accatcagcg
      601 tcgcgagcgc cacgaggagt cgcgcaccag gtctgcggct caccgggatc ctgctcatcg
      661 ccacacccct gacaccggac gaaacggatg aatgcgacgg tacgcgcccg accgccgcaa
      721 cggaaggcaa gcccgtggtg tgatccatca gcgctggagc cagacccgcg agtggcaggc
      781 ccacggcata ccgtggatgc gtgaccacag cgcctgcctt tggcccgctc gcgggcgtga
      841 agatcatcga gctcgccggg atcggccctg ggccgttcgc ggggatggtg ctcgccgatc
      901 tcggggccga cgtgatcggg gtcgaccggc ccggcggcgc gggccccttc gacctgccgg
      961 tgctgcgccg tggccggcgg tcaatcgccg tcgacctcaa gagtgaggcg gggcgcgagg
     1021 tcatcctcga tctcgtcgcg agcgccgacg cgctcatcga accctaccga ccgggtgtca
     1081 tggagcggct cgggctcggc cccgacgccg tcctggcccg caacccgcgg ctggtctatg
     1141 cgcggatgac cggatggggg caggacggac cgcaatcccc gtatgccggt cacgacctca
     1201 cctacaccgc cacctcgggc atcctctcca gcttcgtgcg ggacccgcac gacgggagta
     1261 tgccgcggcc ggtcctgccg gggccctacc tcggcgacgt cgcgggtggg gcgctgctgc
     1321 tcgtcgccgg actgttggcc gcgctgcact cggccgctgc aacgggcacc gggcaggtga
     1381 tcgacgcggc gatcatggac ggcgcgtcct atctgagcac cttcacccat gcgctgcgcc
     1441 tcggagggat gtggggtggc actccgggca acaacatcct cgacacgggc gccccgttct
     1501 acgaggtcta cgagtgcgcg gacgggcgac acctggccgt cggcgccctc gagccgcagt
     1561 tctacgacga gttgctgcgc ctgctcgccg agcatgccgg gctcgacctc gggcccgacg
     1621 acccgcgcca ccggctgaac cggctcgacc agaggcgttg gccggaggcg aaggccttgt
     1681 gggaggactt cttccgcacc cggacccggg acgagtggtg cgcatttctg gagcgctctg
     1741 acgcgtgcgt cgcgccggtg ctctcgctgg aggaagcgcc gacgcacccg cacctgggtg
     1801 cgcgggcggc atacctcccg gtgggtggcg accacgggcc cgagttgccg gtgcccgcgc
     1861 cgcgcttctc gaagacgccc actcagaccc ggccatcccc ggcgaggggt gcccacaccg
     1921 acgaactcct ggccgaagtc ggctaccacc cggcgcggat cgcgacgctt cgcagcacgt
     1981 ccgtggtcgc ctgaacgcca attcctgatc aacgtcctac gatgaggaga ggcgccgtgg
     2041 cccacacggc gatcgggagt cctagggccg gtgaccacca catgagcaag cctgtccagc
     2101 agttggatcg cgtcgtgatc cgattcgccg gtgattccgg cgacgggatg caattgaccg
     2161 gcgaccggtt cacctccgac gcggcgttcc acggcaatga cctgtcgacg ttgcccaact
     2221 tccccgcgga gattcgggcg ccccagggca ccatccacgg ggtgtcgagt ttccagctgc
     2281 acatcgccga ccacgacgtg ctcacccccg gtgacgcgcc cgacgtgctc gttgcgatga
     2341 acccggcggc gctcaaggcc aacctcgccg acctgccgcg cggcgcgacg atcatcgtcg
     2401 acaccgacga gttcagtcgg cgcaacctcg ccaaggtggg gtatgccgtg tcgccgctgg
     2461 acgacggcag cctggagagc taccacgtcc acgccctgcc gctcacctcg atcaccatcg
     2521 acgcgctcgc cgagcacccg ttgacccgca aggacaagga acgggcgaag aacatgttcg
     2581 ccctgggtct gttgtcgtgg ctctacaccc ggccgacgtc gacgaccgag cggttcatcc
     2641 tcagcaagtt cgccaggaag cccgacctgc tggctgccaa cgtggccgcc ctgcgggccg
     2701 gcttcgcctt cggcgagacg accgaggact tcgcccacac ctatcaggtg gccgcggcct
     2761 cgatgcccga cggcacctac cgcaacatca ccggcaacgt cgccctgtcc tacggtctcg
     2821 tcgcggccgc ccaccaggcc ggcctgcgtc tagtcctcgg gtcctacccg atcaccccgg
     2881 cctcggacat cctgcacacg ctgtcgggtc tcaagcggtt cgacgtgacg acgatgcagg
     2941 ccgaggacga gatcgccgga gtcgggatcg ccatcggagc ctccttcggt ggggcgcttg
     3001 gcgtgacgac aacgtcgggt cctgggttgg ccctcaaggc cgaggcgatc ggcctggccg
     3061 tctcgctcga gctccccctc gtggtcgtca acgtccagcg cggtgggccc tcgaccggcc
     3121 ttcccaccaa gaccgagcag gccgacctcc tgcaagcgat gttcggccgc aacggcgagt
     3181 cgccggtcgc gatcgtcgcg ccgcagtccc cgaccgactg cttcgacgcg gccatcgagg
     3241 cgtgccggat cgccacggca taccggacgc cggtcatcct gctgtccgat gggtacctcg
     3301 ccaacgggtc cgagccgtgg aaggtccccg tcccggccga gctgccggcc ctggacgtga
     3361 ccttcacgac cgagcccaac ggggtcgatg ccagcggttc gccggtcttc cagccctatc
     3421 tgcgtgaccc cgagaccctc gcgcgcccgt gggcgatccc ggggacgcct ggcctggagc
     3481 accgcatcgg cggcatcgag aaggccgacg tcaccggcaa catctcctac gaccccgaca
     3541 accacgagct gatgaccaag ttgcgcgccg gcaagatcgc gaaggtcgcc gacaccatcc
     3601 ccggcctgga ggtcgatgac cccgacggcg acgcccgcgt gctcgtcatc gggtggggct
     3661 cgacctacgg tccgatccgt caggcggccc gttcggcgcg tctcaccggg caccgggtcg
     3721 ccacgacaca cctgcgtcac ctcaacccgt tcccgctcaa caccggcgag gtgctcggcc
     3781 gctatgagcg ggtcatcatg cccgagatga acctcgggca atgcgcgatg ctgctgcgcg
     3841 cggcatacct cgtggacgtg cagtcccaca ctgcggtccg cgggctaccg ttcaaagcct
     3901 cggaactcgc cgagatcatc cgcacggccg tcgacggact ggaggcgacg aagtgagcat
     3961 cgacttgggt atgccgtcca gcggggtcgc gttcgtccct cagcttgccg aggggtcgcc
     4021 tgcgctgggg cgcaaggact acacctccga ccaggacgtc cggtggtgcc cgggctgcgg
     4081 cgactacgcg atcctcgcgg cgatgcaggg cttcttgcct gagctcgggc tgcgcaagga
     4141 gaacatcgtc ttcatctccg gcatcgggtg ctcctcgcgg ttcccttact acctggacac
     4201 ctacgggctg cactcgatcc acggtcgggc gccgaccttt gcgaccgggc tggcgacggc
     4261 gcgcgaggac ctgtccgtgt gggtcatcac cggtgacggc gacgcgctgt cgatcggcgg
     4321 caaccacctc atccacgcgc tgcgccgcaa cgtcaacatc aagatcctct tgttcaacaa
     4381 cgagatctac gggctgacca aggggcagta ctcccccacg tcgcgcgcgg ggaccatcac
     4441 caagtcctcc ccgctgggct cgctcgacgc gccgttcacg ccggtctccg tggccctcgg
     4501 ggccgaggcg accttcgtcg cgcggaccat ggactccgag cgcaagcacc tcaccgaggt
     4561 cctgcatgcg gcggccgcgc accgcggtgc ggcgttggtc gagatctatc agaactgccc
     4621 gatcttcaac gacggggcct tcgacgtcct caaggacaag gacgaggcgg ccgcccgaat
     4681 cctttggctc aaggacggcg aggaggtcgc caccggcgac cgcgtggccg tgcgcgctga
     4741 cgacggctcg atccaggtgg tcccgcgggc cgaggccagc ccgacgcgga tcgtccggca
     4801 cagcgtcgac accgagcacc cgtcgtcggc cttcgcgctg tcccgcctta cctctgacgg
     4861 gctgctggcg cacgtgccca tgggtgtgtt ccgggacgtg tcgcggccga cctacgacga
     4921 catggtccgc ggtcaggtct cggctgcggt cgaggccgcc gggggcccgg cgaccgacgc
     4981 cgatctcgcc gcgctgctcg ccggtcggga cacctggacc gtcggcgggt gacccccgcc
     5041 ctcaacccag gctccgaggt tcgacgcggc gcactcttcg gcaccgcggc atacatgctg
     5101 tgggggttct tccccctcta cttccatggg ctgtccccgg cgacgccgct tgaggtgctg
     5161 gctcaccgga tcctgtggag cctgttcttc tgcctgatca tcctggcttg gcgtcgggac
     5221 gtgacgtggg tgcggccgct gttcgcccgt ccgcggttgc tcgcggggtt gactgccgcg
     5281 gccctgctcg tcgcgatcaa ctggggggtc tacgtcgctg cggtgttgag cggacacacc
     5341 cgggacgctg cgctcggcta cttcctcaac ccactcatca ccgtggccct cggcgtcgtg
     5401 ctgctgcgcg aacccctgcg ccgcctgcaa tggatggccg tcgcgatcgg cctggtggcg
     5461 gcggtctttc tcacggtggc cggcgggcag gtgccggtca tcgctctcac gctggcggtg
     5521 acgttcgcgc tctatgggct gctcaagaag cgcatcggcg ccagcctggg ggcgctgcac
     5581 gggctcacga tcgagaccgc cgtgctgctg ccgatcgcgt tgctcctgct cgggccgttg
     5641 tcggggttgg gcggcaccca tgcggggttc ggctcggtcg gtcaagcggc tgggctcatc
     5701 ctcaccggac cgatcacggc gatcccgttg ctgctgttcg ctgccgcggc tcgacgggtg
     5761 cccctcgtga cgatcgggtt gttgcagttc ctcacgccgg tcatgcagtt gctgtgcggc
     5821 ctgttgctcg gtgaggtcgt caccccgacc cagtgggtgg gcttcgtcat cgtctgggtg
     5881 gccctggggg tgctgacggt cgattctttg cggcaggttg cccgaagtcg ctgaggatcg
     5941 gacaatcagg acatgacgat cacccggttg ctccgtgtcc tgctcgtcgc cttcgctgcg
     6001 gggctcgccg tgggcgtcct catggcgcac tggctcagcc tggatgtggc gcagacctac
     6061 cccgaggtcg cccacctgcg cgcgcctgcg ttctgggctg ccgtcgtggg cctcgttcca
     6121 ctcgttgtcg cggccggggc catgtggcgc ttcctcgcgc tcgtggaccg gggtgaggcg
     6181 ttctctggtg ccagtgcgcg gctggtccga cgggtcagcg ttgtcttcct cgtgctggcg
     6241 gtctacatcg cggtggccgc ggtcgtccta ggcggcgcgt tgccgcagtc gagtccgacc
     6301 ttcacgctcg ccgtcatggc ggccgaatcc gtcgcgttgt tcctcatggc tgcctgcgcc
     6361 ctgttgggga ggctgctgcg cgaggccgga ttgccgctcc atcggaaccc cctgccggcg
     6421 tgacgacgca cattgggcgg ggcaggcggg gatgaagccg tccgtgaagt cgccagaggt
     6481 ggtgtgatca cgcatgctgt cgctgcttca cggacggctt cacacccgcg ttcgtgacgt
     6541 tgcgggggtt cagccgacgc gctcgacgac ctggtcaacc agcgctacca gagcagcctt
     6601 cgcgtcgctg tcgggcagcc ccgccaacgc ctcgcgagcc tgcgccccga cctcgcgggt
     6661 gtgcgctcgg gcccgctcca acgccgggtt ggccctcagc agcgccagcg cttcggcatg
     6721 aagcacgtcg tcggtgaggt ctgcggccag caactcctgc aaccgcgcat cggccgggtg
     6781 tgaagacgcc ttcgcgaaca gcacggcgag cgtgtcgact ccctcgcgca ggtcggtgcc
     6841 cggcgtcttg ccgctcatcg ccccgtccga ggcgatgtcg atgatgtcgt cggccaactg
     6901 gaacgccatc cccagccgtt cgccgtagac ccgcaacacc tcgaccatcg ccggtgaccc
     6961 accgccgaac atcgcgccat agcgggcggc cgtcgccacc aaggcacccg tcttgtcggc
     7021 gagcacgccg aggtagtagt cgatcgggtc gacgtcgggc gggcaggccc gggtgtcgcg
     7081 gatctgcccg aaacacaacc gcacgaaggt ctgcgcctgg atgcggacgg cctcggcccc
     7141 cagcccggcg acgatctccg acgccttccc gaacaggagg tcgccgacga ggatggccgt
     7201 ggtgttgtcg tatgccgcgt tgacgctcgg gacggcccga cgcaccgccg cctcgtccat
     7261 cacgtcgtcg tgatagagcg aggccaggtg ggtgagctcg acgcccacgg cagccgcgat
     7321 gacctcatcg gtgacgccgc ggcatacctg cgccgcaagg agcgtcaacg tgggacggaa
     7381 ccgcttcccg cctgcggcga gcaggtgggc cgaggctgcg gcgacgtagg ggtcgtcgtg
     7441 gtccacggtc gaacgcagca gcgcgtcgac gcgccccatc ccgtcggcca gggcagccgc
     7501 caactccggg ctcacccccg gcagggacag cacctggggt gcactcatcg agtcgacttc
     7561 ctggtgtcgg tggcgaccga ggtcaccgaa tgaactgcga cgcccgctcc gcgagctgga
     7621 gcaccgaggc cggcgcgatg ccgaggacca gcgtgaacag cgtgcccgtc gcgaccgtca
     7681 cggtcgtgaa gaccgacggc tcgatgacgg caacggagtc gtcggtggag tcggtgaagt
     7741 acatgagcac gatgatccgg acatagacga acacggtgat cgcgctgcac aacagaccca
     7801 acaccacgag gatcgtgccc gccgtgccgc tttcgagcgc cggcttgaag acggcgaact
     7861 tggcggtgaa ccccgaggtg agcgggatgc ccgcgaacgc cagcagcagg aaggcgaaca
     7921 cgcccgccgt ccacgggtga cgcttgccca ggccggtcca ctgcgagagg tgggtcgcct
     7981 ccgacccgcc ttcgcggacc agacccacga tcgcgaacgc cgcgatggtc gtcagaccgt
     8041 atgccgcgat gtagaacatc acgccgctca ccccgtcgcg ctccatggag aggacgccga
     8101 ccaggatgaa gcccgcgtgc gcgatcgagg agtaggccag cagccgcttc atgtcggtct
     8161 gggtgaccga gaggaccgca ccgatgatca tcgtcagggc ggcgacgaca acgaccgcgg
     8221 gacgccagtc ccactgcgcc cggtccaccc cgacatagac aaagcgcagg atcgcaccga
     8281 acgcggccgc cttggtgccg gcggccatga agccggtgat cggcgtgggg gcaccctgat
     8341 agacgtcggg ggtccacatg tggaacggca ccgcgccgac cttgaagagc aggccgacga
     8401 gcacgaggag cacacccggg accagcaggc cctggaacgc ggacccctgc gccgtgatcg
     8461 cggcggagat gtcgggcagg tagaccgagc cggcgaaacc gtagagcagc gcggtcccga
     8521 agaggaagaa cgccgacgag aaggcgccga gcaggaagta cttgagcgag gcctcctgcg
     8581 acaggaggcg acgccgccgg gccaacccgc agagcaggta gagcggcagc gagaggacct
     8641 caagcgcgac gaacatcgtc agcaggtcac ccgcggccgg gaagagcatc atgccggcga
     8701 ccgagaagag gaacagcggg aagacctctg ccgtcatcgc gccgaccttg cgggcctgcg
     8761 cctcctgcgg cgatcccggc gtcgcggccc ccatgggggt gaacgcgtcg gcgccgacgc
     8821 cgccgaaccg ctcggcgagg ctgagcgcgg ccagcagggc gaacaacagg atggtggcct
     8881 ggatgaacag ggccggcccg tcgatgacga ccgaaccctt gaccgtcccg ttgaacccag
     8941 cgatccctgc ggtgcttacc cggtgggtga ccgccccgaa ggccagcgcg gccatggcgg
     9001 ccacaatgcc gaccagcgtg atggtcacct gggcgaggta gcgcgacgtg cggggcacga
     9061 acgcctcgac gaggacgccg agcatcgccg cactgaagat gatgagaacc ggaaggaggg
     9121 cgacgtagtc gagcgtcgcc ggcgtgaagg ccgcagacag cgcggtggtc acttggtgct
     9181 cccttcggcc gcgccgacgg cgggcgccgg atcggtgacg tggatcgagg tgaggatcgt
     9241 cgtgaccgcc ggggtgacca tgtccagaac cggcttcggg tagaagccga ggatgaggaa
     9301 caaggcgatg agcgggccga cgacccactt ctcccgatag ctgatgtcag gcgtcccggc
     9361 ggcactctcg ggcttcgggc cggtcatcgt gcgctggtac atgagcagga tgtagagcgc
     9421 ggccaggatg atgctcgtcg tcgcgatgac gccgacgacc tggtagcgct ggaacgtccc
     9481 ggccagcacg aggaactccg agatgaacga gccgaggccc ggcagcgcga gaccagacag
     9541 gccgacgagc agccagacac cggccagcgc cggcgtcaca cgctgccacc caccgaagtc
     9601 ggggatccgc ttgctgccgc ggcggctgat gaacatgccc gcgaccagga agaggcccgc
     9661 cgtcgagaag ccgtggttga tcatgtagaa gctcgacccg gcccaactcg tcgaggtcat
     9721 cgcgaagatg cccaggacga tgaagccgaa gtggctgatg gacgtgaacg cgatgagccg
     9781 catgaggtcg gtctgtccga tcgccaacag cgcgccgtag aggatcgaca ccagggcgag
     9841 cgtgatgacc acgggcgtcg cccactggct ggcacccggg aacagctcca ggcagaagcg
     9901 gatcatcccg taggtgccga ccttgtccag gacaccgacg agcagcgtcg cggtggccgg
     9961 tcggctctcc gccgccgcgt cgggcagcca cgtgtgcaca ggcaccatcg gggccttgac
    10021 cgcgaaggcg atgaagaacc cgaggaacag ccacttctcg gttccgggcg ccagttgtat
    10081 gccggccagc ttgctcagca ggaagccgtc ggctccgccg gggccctgga catacagccc
    10141 gatgaccgcg gccagcatga tgagcccgcc ggccagggag aagagcagga acttgacggc
    10201 ggcatactgc cgacgcggcc cgccgaactg cccgatgagg aagtagaccg ggacgagcat
    10261 cgcctcgaag aagacgtaga acaagaacac gtcggtcgca gcaaacacgc cgaccatgaa
    10321 cgtctcgagg acgagcatga gggcgaagta gtggcgctca cgatgcgctt cctcggccgg
    10381 caggtcgttc caggcggcca gcaggcacac cgggacgagg atcaccgaca tgaggatgag
    10441 cgacagggcg attccgtcga caccgagggc gtagctgacc ccgaactgcg ggatccacga
    10501 gtgctcctcg acgagctgga acttgctcgt gctcgacgtc gagaactgca acgcagccgc
    10561 gacggcaaag acgagggtca ccaacgacgc gccgagcgcg atgtgcttgg cccggtcggc
    10621 cagcgaggca ggcagcagcc acaggaccac ggccccgaca agcgggatca ggccgatgac
    10681 ggtcagccag gggaactggt tcatcactgc accacccaga cggcaccgag gatcacgacg
    10741 acgccgatga gcatcgtcag ggcataggag cgggccaggc cgttctggac cctacgcaaa
    10801 cgagaggacg taccgccgat gagggccgcg aaacctccgg ccgccccatc gatcccgcgc
    10861 ttgtccagga agacgagcgc gcgggtgagg tggatgccgg ggcgcatgaa gacgccctcg
    10921 ttgacctggt cttggtagag gtccgctcgg gccgcacggg tgagcaggct gcccaccggt
    10981 gccgttgtcg ggaccttgtc acgccagtag cgcatccagg ccagcgcgac gccggccgcg
    11041 acgacgagga gcgtgatcgc catgagggcg gcgacaggga tcaccgggtg ctcctcctct
    11101 gcggccccga cgaccggctc aagccagtgc aggatgccgt tggtcgggcc gaggatgagg
    11161 ccgagagagg ccgaccccac cgccagcacc atcatgggga tggtcatcgt ccgaggtgac
    11221 tcgtgcgggt gagcgtcctt gggccaacgc ttctcaccct ggaaggtcat gaagaacaac
    11281 cgggacatgt agaacgcggt gatcccggcg ccgatgaggg cagccagtcc gaacacccac
    11341 ggccgccagc cctcgccgac gaaggccgcc tcgatgatct tgtccttgct ccagaagcca
    11401 gcgaacggcg gcacaccgag gatggctagc cagcccaagc cgaaggtcac ccaggtgatc
    11461 ttcatgacgc tcgacaggtt gccgaagccc cgcatgtcga ccttgtcgtt catgccatgc
    11521 atgaccgagc cggcgccgag gaacatcccg gccttgaaga agccgtgggt gagcaggtgg
    11581 aagatcgcga acgcgtaacc gaccgggccg agaccggccg ccagcatcat gtagccgatc
    11641 tggctcatcg tcgaggcggc cagcgccttc ttgatgtcgt ccttggcgca tccgacgatc
    11701 gccccgtaga cgagcgtgat cgcgccgacg atgaccacgg ccaactgcgc gttgggtgcg
    11761 ccgtcgaaga tcgcgtggct gcgcaccacg aggtagacac cggcggtgac catcgtcgcg
    11821 gcgtggatga gtgccgacac cggggtcgga ccggccatcg catcgccaag ccagctctgc
    11881 agggggaact gggccgactt gccgcaggcg gccagcagca ggaagagacc gatgacggtc
    11941 aaggcgccct cgttggcctc ggccacagca ccgttgacgg tctggaagtc gacagccccg
    12001 aactgccaga acatcgccat gatggccagg cccatgccga cgtcaccgac gcggttggcg
    12061 acgaacgcct tgttggcggc gctcgcgtag ggcgggttcc agttccagaa gccgatgagc
    12121 aggtaggacg ccaggccgac gccttcccag ccgacataga ccagcaggta ggagttggcc
    12181 agcaccagca ggagcatggc ggcgacgaag aggttgaggt aagcgaagaa ccgccgcttg
    12241 tcggggtcgt gctccatgta acccagcgag tagacgtgga tgagcgaccc gacgaaggtc
    12301 accagcagca cgaaggcgat cgagagcggg tcgagcaaca tcccggcctc gagctggaac
    12361 tgcccggccg gcacccagtt gaacaggtgc accgtcaagg cccgctcttc cgagctcaag
    12421 cccagcaacg tgataaacac gccgagcccg acgacgaacg cggcccagga catggcggtc
    12481 gcgaacagcg gcccccactt gtcggtgcgg tggccgccga gcagcaggac ggcagcgccc
    12541 agcaggggca gcgccacgag cagccaggcg gccgacgcga agccagtggc cgcgtggacc
    12601 gtggacgctt cgcccgcgaa cggcatacct gcgggcaggc ctgccatggt ggtcagagag
    12661 acgctcactc gtttctccct tacagcttca gcaggttggc gtcgtcgacc gaggccgatc
    12721 ggcgggcacg gaagatggcc atgatgatcg ccagaccgac gacgacctca gcagcggcca
    12781 cgaccatgac gaacagcgcg atgacctggc cgtcgagggt tccgtggatg cgtgagaagg
    12841 tcacgaaggc gaggttggct gcgttgagca tcaactcgat gcccatgaag atgatgatcg
    12901 cgttgcgccg caccaggacg gtggccgccc cgatcgtgaa gagcaggatc gacaggtaga
    12961 tgtagttgat gaggttcacc gcgacctccc ctcctcgatc tcggcactgg catgggccgt
    13021 cgaggtcccg tcgggggcga cgatctgctc acgcgcgatg aggacccgcg agacggacaa
    13081 ctcgctcggt gtgccgtcgg gcagcaacgc gggggtgtcg accgagttgg accgggcgta
    13141 gacaccggga gccggcaaac cggcgacgaa cacgttgtcc ttgacccgcc gagccgccaa
    13201 ctcgcgctgg gtcgacttgg gcgtgagtcg ctcgcggtgg gccagcacca tggcgcccac
    13261 ggcagccgtg atgagcagcg ccgaggtgat ctcgaagacc cagacgtact tgccgaagat
    13321 gaggtcggcc aacccactga cgttgcccgg gtcgacgttg accttgtcga ggccggccg
//
LOCUS       DAOMZY010000047        13342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_4275_length_13342_cov_5.723414, whole genome shotgun sequence.
ACCESSION   DAOMZY010000047 DAOMZY010000000
VERSION     DAOMZY010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 13342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13342
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4275_length_13342_cov_5.723414"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..293)
                     /locus_tag="PLK64_04325"
     CDS             complement(39..293)
                     /locus_tag="PLK64_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HPV78999.1"
                     /translation="MSDAFQDAVAAVGTLTKDPGPQVKLALYALFKQASEGDVKGKRP
                     GFTDFVGRAKYDAWAALSGTSAEQAKADYIAKVAELQAAE"
     gene            complement(341..2101)
                     /gene="arc"
                     /locus_tag="PLK64_04330"
     CDS             complement(341..2101)
                     /gene="arc"
                     /locus_tag="PLK64_04330"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HPV79000.1"
                     /translation="MAQEPTPHPATDDHAEAVAAQVAAEITHLQAEVAELRRGRTDPA
                     RVRELEHRIAAQASSMATLSAQNERLVRTLKDARSQIVNLKSEVDRLAQPPNAYGLVL
                     EPAADGVVDILTSGRKMRVAVGAAIESQVLPGREVMLNEALNVVAVKGYEKVGEVVLV
                     KEVLDDDRVLVVAHADEERVCRRAETLRGEHIRVGDALTIDPRSGFVFERVPKAEVQD
                     LVLEEVPDIAYEDIGGLAGQIEAIRDAVELPYLHPELYREHHLKPPKGVLLYGPPGCG
                     KTLIAKAVAASLARKVAEKTGKPVEKSYFLNIKGPELLNKYVGETERHIRLIFQRARE
                     KASDGTPVVVFFDEMDSLFRTRGSGVSSDVETTIVPQLLSEIDGVERLENVIVIGASN
                     REDMIDPAILRPGRLDAKIKIERPDREAARDIFSKYLTVDLPLHEDDVAEHGGDRERC
                     VEAMIQTAVERMYAESDENRFLEVTYAGGDKEILYFKDFNSGAMIQNVVDRAKKNAIK
                     EFLSTGQKGVRIEHLLSSCVDEFKENEDLPNTTNPDDWARISGKKGERIVYIRTLINT
                     KDGTEPGRSISNVENTGQYL"
     gene            complement(2206..3252)
                     /locus_tag="PLK64_04335"
     CDS             complement(2206..3252)
                     /locus_tag="PLK64_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HPV79001.1"
                     /translation="MTAEPRRGTQAQRGAFVVGDRVQLTDAKGRLHTITLEAGREFHT
                     HQGRLAHDELIGAPEGSVVANSAGVEYLALRPLLADYVMSMPRGAAVIYPKDAGQIVA
                     MGDIHPGARVVEAGVGSGALSMSLLRAVGDEGELISFERRADFAGIAQGNVRAFFGRE
                     HPAWRVVVGDLVESLPGEVEPDSVDRVVLDMLAPWECIDVVATALRPGGVLVCYVATT
                     TQLSRVAEALREHSGWTEPSAWESLVRGWHLEGLAVRPEHRMHGHTGFLISARRLADG
                     VVTPRRTRRPAKGAYGETGADGIPDGGGEASGSEAVAGVADADWTPEALGERPISDRK
                     VRRAHRTVTGTSAE"
     gene            complement(3264..3608)
                     /locus_tag="PLK64_04340"
     CDS             complement(3264..3608)
                     /locus_tag="PLK64_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPV79002.1"
                     /translation="MAAERAEDPVLNEVHLAGRVGSEPEERELPSGDLVVTLRLVVPR
                     GAARRSPRGPTVDAIDVSCWTAATRRSALRLRPNDQAVVDGSLRRRFFRTGGGVQSRY
                     EVEAVKVRRRAT"
     gene            complement(3781..4914)
                     /locus_tag="PLK64_04345"
     CDS             complement(3781..4914)
                     /locus_tag="PLK64_04345"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HPV79003.1"
                     /translation="MSPAADPQYGWLLGRVAGIPVYIGRSWPIIAVVVVMTFGPQLAG
                     ALPGGAGYAVAAAYALLLLVSVLVHEAGHALAARSTGSEVSRIVANLWGGHTVFGSGQ
                     RRPGTAALIAIAGPAGNLLLAGVGWLLLQVDLPAVPALLAWALAFSNVFVAVFNLLPG
                     LPLDGGFVVEAVVWKVTGDRWLAMVVAGWLGRVVTVLAVVYLLAEPLLSGGRPSLVTI
                     VWVALIGAFLWQGATQAIRAGTARRAVARVRVGQVLRPVVVVPGSASAATALEGFARL
                     PGAERAVLVDPMGTPVGFLDLAALSAVPVDQLPVVPATAVLVRPPSGWVVQAEPGDDA
                     SLVVSALAGHVEGENVKEAVLVCDSLGRVIGTVSLADVEAALR"
     gene            5077..8922
                     /gene="metH"
                     /locus_tag="PLK64_04350"
     CDS             5077..8922
                     /gene="metH"
                     /locus_tag="PLK64_04350"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HPV79004.1"
                     /translation="MTAPETVSAPVRATEVPSSPRPDATVALTQALRTRILVLDGAMG
                     TLIQRHELTEADYRGARFADWGQDLKGNNDLLTITQPQIIADIHRAYLNAGSDLIETN
                     TFNANRISLADYGMSDLAYELNIAAAALARTEADAATARTPGQPRYVAGALGPTNKTA
                     SISPDVNDPGARNVSFDELVEAYLEQAGGLVDGGADVLLIETIFDTLNAKAAIFALET
                     LFEQRARRWPVVISGTITDASGRTLSGQVTEAFWNSVRHARPLAIGLNCALGADEMRP
                     YVAELSRVADTFVSCYPNAGLPNAFGEYDESPETMSGVVGEFASSGLVNIIGGCCGTT
                     PEHVAQLVAAVAGMPPRSVPEVPSATRLAGLEPLTIDEQSLFVNVGERTNITGSARFR
                     KLIQAGDYGAALTVARQQVEAGAQIIDVNMDEGMIDGVAAMDRFLKLVASEPDICRVP
                     VMVDSSKWEVIEAGLKTIQGKPIVNSISMKEGVEAFVKHARLCRKYGAAIVVMAFDEQ
                     GQADNLARRREISAAAYRILVDEVGYPAEDIVIDPNVFAVATGIEEHSAYGIDFIEAT
                     RWIKENLPGALVSGGVSNVSFSFRGNNPVREAIHAVFLLHAIRAGMDLGIVNAGALVV
                     YDEIDAELRERIEDVILNRRADAAERLLEVAERFNVAGTQAEVADEAWRSLPVGQRIT
                     HALVKGLDAHIEADTEELRADLAASGGRPIEVIEGPLMDGMNVVGDLFGAGKMFLPQV
                     VKSARVMKKAVGYLVPFIEAERLAAGGSADDAEAHTNGTIVMATVKGDVHDIGKNIVG
                     VVLQCNNYEVIDLGVMVPAQKIIDAAREHRADIIGLSGLITPSLDEMVTFAAELQRLG
                     LTLPLLIGGATTSKAHTAVKVSPRYDGPVIWVKDASRSVPVAAALLSDEQRPGLLADV
                     KADYDAIRVRHAAKAGDRQLISLEAARANATPVDWAAYKPLRPHLLLQQARDVSAATS
                     GANPTHVTQFTRVFRDYPVAELREYIDWQPFFAAWELKGRFPDLLNNPASGETARKLY
                     ADALTMLDRIEAERWLRPAGVIGLFPANSVGEDIEVYAAESRSEVRTVLHHLRQQGAH
                     RDGIPNRSNADFVAPKSTGLRDYMGAFAVTAGVDLPARVAAFKADLDDYSAILLESLA
                     DRLAEAFAERLHQRVRREFWGYHPGERLGNEALIAEDYQGIRPAPGYPACPDHTEKAL
                     LWDLLDVEAATGMVLTESMAMWPGASVSGWYFAAPHAQYFVVGRLGRDQIAEYADRKG
                     WTIAQAERWLSPNLGYDPE"
     gene            complement(8953..10044)
                     /locus_tag="PLK64_04355"
     CDS             complement(8953..10044)
                     /locus_tag="PLK64_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPV79005.1"
                     /translation="MATAIRVEMLPARLGDCLLVECLRQGRRPWRMLVDGGPPDTWPL
                     LKARLDRLPASNPAIDVAVVTHIDSDHIGGMIPFLASDFARTLVGDVWFNGHRQASSP
                     RAVPRSVAQGETLTATLSGAPGAQPGMPWNVAFAGAAIATPVDPGFAEVTIPGGPRIT
                     VLSPNGRRLAALAKVWTEALDKALHPEPDRPVPDLPGPLGDLATLAARKTSKDGTPPN
                     GSSIALLVEHRGASVLLGADAFGPVLTDALTALAGARGIPAVAVDAFKLPHHGSEANV
                     LADLVAAAPATHYLVSSNGDTFGHPDDPAIARVVTGAPPGATVWFNYRNARTERWADP
                     ALRTAHGYAVRFPSDPAAGVLVKLPVRAG"
     gene            10148..10993
                     /locus_tag="PLK64_04360"
     CDS             10148..10993
                     /locus_tag="PLK64_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPV79006.1"
                     /translation="MVAALSPSRASDFKQCPLLYRFRVIDRLPSPTSAAAARGTLVHA
                     VLERLFDLPAHERTPAAAAALVAPQWERLVAEEPELAGLLDSDDDGAADAWYAQAVGL
                     VERWFTLEDPTRLEPAERELYVEAEVDGLVLRGYVDRLDVAPDGSMRVVDYKTGRSPS
                     ELFERSSLFQMKFYALVLWRLRGVVPRMLQLVYLGNGEVVRYEPDEADLLATERGIKA
                     LWAAIERAAATGDWRPRVSRLCDWCDHKSLCPEWGGTPPPLPTDAPARALDPAVTGRV
                     VPADD"
     gene            complement(11013..11693)
                     /locus_tag="PLK64_04365"
     CDS             complement(11013..11693)
                     /locus_tag="PLK64_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPV79007.1"
                     /translation="MSARLPAAVLWDLDGTLVDSEAYWVLEERALVEDFGGVWTEADA
                     FALVGRALTDSAAYIKDHSPVDLPIDELVDRLVTGVVRRIRAEVPWRPGARELLTALR
                     EAGVPTALVTMSYRPITDAIGDALPGGAFDLIVSGEDVTHGKPHPEPYLRGAAGLGLA
                     PEECIAIEDSEPGARSAAAAGAQTIVVPSVKPVPRMPGVVQIPTLVGVSPTDLVGLPG
                     FAALPTRS"
     gene            11873..12715
                     /locus_tag="PLK64_04370"
     CDS             11873..12715
                     /locus_tag="PLK64_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HPV79008.1"
                     /translation="MLRSIPELREPVLVAAFEGWNDAGEAASAAAAHLIEVWDAQQVG
                     ELDPEDYYDFQVNRPKITLVDGQRRITWPTTRIFVCQGTTGPHDIVIVEGIEPSIRWR
                     HYADELVEVAQALGVQTVITLGAYLADVPHTRAIPINTSSDDVSVQDRFGAEASEYQG
                     PTGIVGVLTEAVRAAGIESLSCWAAVPHYAGGPPSPKAALALLGRLEEVLDVSIPARA
                     IADEAKAWERGVDEMAESDEEVADYVQSLEEATDTAELPEASGDAIAREFERYLRGHE
                     DDPS"
     gene            complement(12745..>13342)
                     /locus_tag="PLK64_04375"
     CDS             complement(12745..>13342)
                     /locus_tag="PLK64_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPV79009.1"
                     /translation="WDGATLGRGRPGWHIECTALAVRYLGMPFTVQGGGTDLVFPHHE
                     MSAVQGCALTGTRPFAQVYMHQAMVAFEGEKMSKSKGNLVLVSALRAAGVDPMVIRLA
                     LLDHHYRAEWEWIDADLHRATQRLAGWRTALSGNGGAPVGGLLAKLRERVADDLDTPG
                     ALALVDAWAVRTLEHGGTELGAPGLVARAVDRILGVRV"
BASE COUNT         2070 a         4775 c         4492 g         2005 t
ORIGIN      
        1 cctcgccgcc gaaagtagcg gctggctatg agggctgcct actcggcggc ttggagctcg
       61 gcgaccttgg cgatgtagtc agccttggcc tgctcggccg acgtaccgga cagggcagcc
      121 cacgcgtcgt acttcgcacg gcccacgaag tcggtgaagc cagggcgctt gcccttgacg
      181 tcgccctcag aggcctgctt gaagagcgcg tagagggcca acttgacctg cggaccgggg
      241 tccttggtca gggtgccgac ggctgccacc gcgtcctgga atgcatcgct catgggctca
      301 ccctaggtgc cacgctgcag ccccgccacg gggacatcgc ttacagatat tggccggtgt
      361 tctccacgtt gctgatcgac cggccaggct cagtgccgtc cttggtgttg atgagggtgc
      421 ggatgtaaac gatccgctcc cccttcttgc ccgagatccg ggcccagtcg tccgggttgg
      481 tcgtgttggg caggtcttcg ttctccttga actcatccac acaggacgag agcaggtgct
      541 cgatccgcac gcccttctgg ccggtcgaga ggaactcctt gatggcgttc ttcttggccc
      601 ggtcgacgac gttctggatc atcgcgccgg agttgaagtc cttgaagtag aggatctcct
      661 tgtcgccccc ggcgtacgtc acctcgagga agcggttctc gtcgctctcg gcatacatcc
      721 gctcgactgc ggtctggatc atcgcctcga cgcagcgctc ccgatcgcca ccgtgctcgg
      781 cgacgtcgtc ctcgtgcagg ggcaggtcga cggtcaggta cttggagaag atgtcgcgcg
      841 ccgcctcgcg gtcgggacgc tcgatcttga tcttggcgtc caaccggccc ggacgcagga
      901 tcgccgggtc gatcatgtcc tcgcggttgg aggcaccgat gacgatgacg ttctccagcc
      961 gctcgacgcc gtcgatctcg gagagcaact gcggcacgat cgtcgtctcg acgtcgctgg
     1021 acacgcccga cccgcgggtg cggaacagcg aatccatctc atcgaagaac accaccacgg
     1081 gcgtcccgtc cgaggccttc tcacgagccc gctggaagat caaccggatg tgccgctcag
     1141 tctccccgac gtacttgttg agcagttcgg ggcccttgat gttgaggaag tagctcttct
     1201 caaccggctt cccggtcttc tccgcgacct tgcgggccaa cgacgccgcc acggccttgg
     1261 cgatgagggt cttcccgcag ccgggaggcc cgtaaagcag gacgcccttc ggcggcttga
     1321 ggtggtgctc gcggtagagc tcggggtgca ggtaaggaag ctcgacggcg tcccgaatcg
     1381 cctcgatctg cccggccagc ccgccgatgt cctcgtaggc gatgtcgggg acctcctcca
     1441 gcacgaggtc ctgaacctcc gccttaggca cccgttcgaa gacgaaaccc gacctcgggt
     1501 cgatcgtcag cgcgtcaccg acgcggatgt gttcgccgcg cagcgtctcg gcgcgacggc
     1561 atacccgctc ctcgtcggcg tgcgcgacga ccaggacacg gtcgtcgtca agcacttcct
     1621 tgaccaggac gacctcgccg accttctcgt agcccttgac ggcgacgacg ttgagggcct
     1681 cgttgagcat gacctcgcgg ccaggcagga cctgcgactc gatcgccgca ccgacggcga
     1741 cgcgcatctt gcgaccggag gtgaggatgt cgacgacacc gtctgcggcc ggctcgagga
     1801 ccaggccata cgcgttcggc ggttgcgcga gccggtcgac ctcggacttg aggttgacga
     1861 tctggctgcg agcgtccttg agcgtgcgca cgaggcgctc attctgggcc gagagggtgg
     1921 ccatcgagct ggcctgcgcg gcgatccggt gttcgagctc gcggacgcgg gccgggtcgg
     1981 tgcgccctcg gcggagctcg gccacctcgg cctgcaggtg ggtgatctcg gccgccactt
     2041 gggcggcaac ggcttcggca tggtcgtcgg tggccgggtg cggagtgggc tcctgtgcca
     2101 tgacgcctcc tgtcctgggt tgggccgtcg ccgggggaac gcggtcggtc agcggtgctc
     2161 tcttgacact aacccccgac tcttgacatt aactcccgcc ggcccctact ccgcggacgt
     2221 tccggtcaca gttcggtgtg cccgtctcac cttccggtcg ctgatggggc gttcgccgag
     2281 cgcttcgggc gtccagtcgg cgtcagccac gcccgcgacc gcctcagacc cgctcgcctc
     2341 gccaccccca tcgggtatgc cgtccgcccc cgtttcgccg taggccccct tcgcggggcg
     2401 gcgcgtccgg cgcggagtga ccaccccgtc cgccagccgc cgagccgaga tgaggaagcc
     2461 ggtgtggcca tgcatccggt gctccggacg gaccgccaaa ccctccagat gccagccgcg
     2521 cacaagcgac tcccaggccg acggctcggt ccacccgctg tgctcgcgca acgcctcggc
     2581 aacccgggac aactgggtcg tcgtcgcgac gtaacagacg agcactcccc cgggccgcaa
     2641 cgccgtggcg acgacgtcga tgcactccca gggagcgagc atgtccagga cgacccggtc
     2701 cacgctgtcg ggctccacct caccgggcaa cgactcgacc aggtcgccga ccaccacgcg
     2761 ccacgccggg tgctcgcgcc cgaagaaggc cctgacattg ccctgggcga tcccggcgaa
     2821 gtcggcccga cgctcgaagg agatcaactc cccctcgtcg cccacggcac ggagcagcga
     2881 catcgacagt gccccggaac cgacgccggc ctcgacaacg cgggcgcccg ggtggatgtc
     2941 gcccatcgcg acgatctgcc cggcgtcctt gggatagatg accgcagcgc cgcgtggcat
     3001 cgacatgacg taatcggcta gcagcggtcg cagcgcgagg tattcgaccc ccgctgagtt
     3061 ggcgacgacg ctgccctcgg gtgccccgat cagctcgtcg tgggccagcc gaccctggtg
     3121 ggtgtggaac tcgcggcccg cctccagggt gatcgtgtgc aagcgtccct tggcatcggt
     3181 gagttgcacc cgatcaccga cgacgaacgc ccctcgctgg gcttgggtgc ctcgcctcgg
     3241 ctctgcagtc atggcgagtg agtctaggtg gcccgccgcc gcaccttcac agcctccacc
     3301 tcgtatcggc tctgcactcc cccgccggta cggaagaacc tccggcgcaa cgagccatcg
     3361 acaacggcct gatcgttggg acgtaggcgc agcgccgagc gtctcgtggc cgccgtccag
     3421 caggagacgt cgatcgcgtc cactgtcggg ccgcgcggtg atcgccgcgc ggcccctcgt
     3481 ggcacgacga gccggagcgt caccaccagg tcgccgctgg gcaactcccg ctcctccggc
     3541 tctgaaccca cccgaccggc caagtggacc tcgttgagca cgggatcctc cgcgcgttcc
     3601 gccgccatct cgactcctcc cggtgcccgc gggcccgtgt cgcgggcgcc gtgtggtcgc
     3661 gccgacaccg acacctcaca cccaccagct gggtgaatcc agcgctccag gggaacctgt
     3721 ggacagtggg acgaccgacc acgggcatgt ggacaacgca gagccagccc ccacaaaggc
     3781 tcagcgcaga gcggcctcga catcggcgag tgagacggtc ccgatcacgc ggcccaacga
     3841 gtcgcagacc agcacggcct ccttgacatt ctcaccctcg acatggccgg cgagcgcgga
     3901 aacgaccaaa gacgcatcgt caccaggctc ggcctgcacg acccaccccg agggcggacg
     3961 aaccaagacc gccgtcgcag gaaccacagg caactgatcg acgggcaccg cactcaaggc
     4021 ggccaggtcc aggaacccca cgggcgtccc catcgggtcg accaggacgg cgcgctcggc
     4081 ccccggcagc cgcgcgaacc cttccagcgc agtggccgcg ctggcagacc ccggcacgac
     4141 gacgaccgga cgcagcacct gtcccacccg cacccgcgcc accgcccggc gggccgtccc
     4201 cgcgcggatc gcctgggtgg ccccctgcca gaggaacgcc ccgatgagcg cgacccacac
     4261 gatcgtcacc agggagggtc gaccgcctga cagcaggggc tcggcgagga ggtagacgac
     4321 cgcaagcacc gtgacgacgc ggcccaacca tcccgcgacg accatggcca gccaccggtc
     4381 gccggtgacc ttccagacca cggcctcgac gacgaagcca ccgtccagcg gcagccccgg
     4441 cagcaggttg aagaccgcaa caaatacgtt cgagaaggcc aacgcccagg cgagcagcgc
     4501 tggcactgcg ggcaggtcga cctgcaacag gagccacccg actcccgcaa gcagcaggtt
     4561 ccccgcgggc cccgcgatcg cgatcaatgc tgcggtcccc ggccgacgct gccccgaccc
     4621 aaagacggtg tgcccgcccc agaggttcgc gacgatccga ctgacctcgc tgccggtcga
     4681 ccgagccgcg agcgcgtggc ccgcttcgtg gacgagcacc gacaccaaca acagcagcgc
     4741 gtatgccgcc gcgaccgcat accccgcgcc tcccggaagc gctccggcca gttgcgggcc
     4801 gaaggtcatc acgacgacga cggcgatgat cggccaggac cgaccgatat agacaggtat
     4861 gccggccact cgccccagca accagccgta ctgaggatct gcggccgggc tcatggtcgc
     4921 aaaccctacg tcaccaccct ctccgctcct gcccgctcca cccggcccaa gcccgccccg
     4981 ccgcccccaa cgtccgggtc gcggtcgatc ccccgcaggc gtccgcgtgc gcggcatact
     5041 ggtccggccg ccccgcccac cccaccaagg acccttgtga ccgcgcccga aaccgtgtca
     5101 gcgcccgtgc gagcgacgga ggtgcccagc agcccccgtc ccgacgcgac tgtcgcgctc
     5161 acgcaggcgc tgcgcacccg gatcctcgtc ctggacggcg cgatgggcac cctcatccag
     5221 cgccacgagc tgaccgaggc cgactaccgc ggggcccggt tcgccgactg gggtcaggac
     5281 ctcaagggca acaacgacct gctgacgatc acccagccgc agatcatcgc cgacatccac
     5341 cgggcctacc tcaatgcagg cagcgacctc atcgagacca acacgttcaa cgccaaccgg
     5401 atctcgctgg ccgactacgg catgtccgac ctcgcctacg agctcaacat cgctgcggcg
     5461 gcactcgccc gcaccgaggc cgacgcggcg accgcccgca cccccggcca accgcgctat
     5521 gtcgccggag cgctcggccc caccaacaag accgcgtcta tctcccccga cgtcaacgat
     5581 cctggcgccc gcaacgtcag cttcgacgag ttggtcgagg cctacctcga gcaggccggc
     5641 gggctggtcg acggcggcgc cgacgtgctg ctcatcgaga ccatcttcga cacgctcaac
     5701 gccaaggcgg cgatcttcgc cctggagacc ctcttcgagc agcgcgcacg ccgttggccg
     5761 gtcgtcatct ccggcacgat caccgacgcc tccggccgca cgctctcagg tcaggtcacc
     5821 gaggccttct ggaactccgt ccgccacgcc cggccgctgg cgatcggcct caactgcgcc
     5881 ctcggcgccg acgagatgcg cccctatgtc gctgagctct cccgggtcgc cgacaccttc
     5941 gtctcgtgtt accccaacgc cggcctgccc aacgcgttcg gtgaatacga cgagagcccg
     6001 gagaccatgt ccggcgtcgt cggcgagttc gcgtcatcgg gcctggtcaa catcatcggc
     6061 ggctgctgcg gcacgacccc cgagcacgtc gcgcagttgg tcgccgccgt cgccggtatg
     6121 ccgccccgct cggttcccga ggtcccgtcg gccacccggc tggccgggtt ggagccgttg
     6181 acgatcgacg agcagtcgct gttcgtcaac gtcggcgagc ggaccaacat caccggctcg
     6241 gcgcgcttcc gcaagctcat ccaggccggc gactacggcg ccgccctcac cgtcgcccgg
     6301 cagcaggtcg aggccggcgc tcagatcatc gacgtcaaca tggacgaagg aatgatcgac
     6361 ggggtcgcgg ccatggaccg cttcctcaag ttggtggcca gcgagccgga catctgccgg
     6421 gttcccgtca tggtcgactc gtccaagtgg gaggtcatcg aggccggcct caagaccatc
     6481 cagggcaagc cgatcgtcaa ctcgatctcc atgaaggaag gcgtcgaggc gttcgtcaag
     6541 cacgcccggc tctgccgcaa atacggcgcc gccatcgtcg tcatggcctt cgacgagcag
     6601 ggccaggccg acaacctggc gcgccgacgc gagatcagcg ccgcggcata ccggatcctt
     6661 gtcgacgagg tcggctaccc ggccgaagac atcgtcatcg accccaacgt cttcgcggtg
     6721 gcgaccggca tcgaggagca ctcggcctac gggatcgact tcatcgaggc cacccggtgg
     6781 atcaaggaga acctccccgg cgcgttggtg tccggcggcg tctccaacgt gtccttcagc
     6841 ttccgcggca acaacccggt ccgtgaggcg atccacgcgg tgttcctcct gcacgcgatc
     6901 cgcgccggga tggacctcgg gatcgtcaac gccggtgccc tggtcgtcta cgacgagatc
     6961 gacgccgagc tgcgcgagcg catcgaggac gtcatcctca accgccgggc cgacgccgcc
     7021 gaacgcctgc tcgaggtggc cgagcgtttc aacgtcgcgg gcacccaggc cgaggtcgcc
     7081 gacgaggcct ggcggtccct gccggtgggt caacggatca cccacgccct cgtcaagggt
     7141 ctcgacgcgc acatcgaggc cgacaccgag gagttgcgtg ccgacctcgc cgccagcggt
     7201 ggccgaccga tcgaggtcat cgaaggcccg ctgatggacg gcatgaacgt cgtcggcgac
     7261 ctcttcggcg ccggcaagat gttcctcccg caggtcgtca agtcggctcg ggtgatgaag
     7321 aaggccgtcg gttatctcgt ccccttcatc gaggccgagc ggctcgcggc gggcggcagc
     7381 gccgacgacg cggaagcgca caccaacggc acgatcgtca tggcgacagt caagggcgac
     7441 gtgcacgaca tcggcaagaa catcgtcggg gtcgtcttgc agtgcaacaa ctacgaggtc
     7501 atcgacctcg gcgtcatggt gcccgcccag aagatcatcg atgccgcccg cgagcaccgg
     7561 gccgacatca tcgggctctc cggcctcatc acgcccagcc tcgacgagat ggtcaccttc
     7621 gccgccgagt tgcagcggct cgggctgact ttgccgttgc tcatcggcgg ggcgacgacc
     7681 tcaaaggccc acacggccgt caaggtgtcc ccgcgctacg acggtccggt catctgggtc
     7741 aaggacgcct cccgatcggt gccggtggcg gccgcgctgc tctccgatga gcagcgaccc
     7801 gggctgctgg ccgacgtcaa ggccgactac gacgcgatcc gggttcggca tgccgccaaa
     7861 gccggtgacc ggcagttgat ctcgttggaa gcggcccggg ccaacgcgac cccggtggac
     7921 tgggcggcat acaagccgct gcggccccac ctgctgctgc agcaggcccg cgacgtctcc
     7981 gcggcaacct cgggcgcgaa ccccactcac gtcacccagt tcacccgcgt gttccgcgac
     8041 taccccgtgg ccgagttgcg cgaatacatc gactggcagc cgtttttcgc cgcgtgggag
     8101 ctcaagggtc gcttccccga cctgctcaac aaccccgcca gcggcgagac cgcgcgcaag
     8161 ctctacgccg acgcgctgac catgctcgac cggatcgagg ctgagcgttg gctgcggccg
     8221 gcgggcgtca tcgggctgtt ccccgccaac tccgtgggcg aggacatcga ggtgtatgcc
     8281 gcggagtccc gttccgaggt ccgcaccgtc ctgcaccacc tgcgtcagca gggggcgcac
     8341 cgcgacggca taccgaatcg gtccaacgcc gacttcgtcg ccccgaagtc cacgggcctg
     8401 cgcgactaca tgggcgcctt cgcggtgacc gctggcgtcg acctgcccgc gcgggtggcc
     8461 gcgttcaagg ccgacctgga cgactattcg gcgatcctgc tggagtctct cgccgaccgg
     8521 ctcgccgagg ccttcgccga gcgtctgcac cagcgggtcc ggcgcgagtt ctggggttat
     8581 caccctggag agcggctggg caacgaggcc ctcatcgccg aggactacca aggcatccgg
     8641 ccggctccgg gctacccggc ctgccccgac cacaccgaga aggccctcct gtgggacctg
     8701 ctcgatgtcg aggcggccac ggggatggtg ctcaccgagt cgatggcaat gtggccggga
     8761 gcctcggtgt ccgggtggta cttcgccgca ccgcacgcgc agtacttcgt cgtcggccgc
     8821 cttgggcgcg accagatcgc cgagtatgcc gaccgcaagg ggtggacgat cgcccaggcc
     8881 gagcgctggc tgtcgccgaa cctcggctac gacccggagt aggccggggt aagtcggagc
     8941 gggccggagt agtcagcccg ctcgcaccgg aagcttcacc aggaccccgg cggcggggtc
     9001 gctgggaaag cgcacggcat acccatgggc ggtcctcagc gcggggtcgg cccaacgctc
     9061 ggtgcgggcg ttccggtagt tgaaccacac ggtcgccccc ggcggtgctc cggtgacgac
     9121 ccgcgcgatc gccgggtcgt ctgggtggcc gaaggtgtcg ccgttgctcg agacgaggta
     9181 gtgcgtcgcg ggcgcggccg cgacgaggtc ggcgaggacg ttcgcctcac tgccgtggtg
     9241 cggcagtttg aaggcatcaa ccgcaaccgc aggtatgccg cgcgccccgg cgagggcggt
     9301 cagcgcatcg gtgaggacgg gtccgaacgc gtcggcgcca aggagcacgc tggcgccgcg
     9361 gtgctcgacg agcagggcga tgctgctgcc gttgggtggg gtgccgtcct tcgaggtctt
     9421 gcgggcagcg agggtggcca gatcacccaa cgggccgggc aggtcgggga ccggtcggtc
     9481 gggctcggga tgcagggcct tgtccagggc ctcggtccag accttggcga gggcagccag
     9541 gcggcggccg ttcggggaca ggacggtgat gcgaggcccg ccagggatcg tcacctccgc
     9601 gaagcccgga tcgaccgggg tggcgatggc ggcgcctgcg aaggcgacgt tccacggcat
     9661 acctggctgg gctccgggag caccgctcaa ggtggccgtg agcgtttctc cttgggcgac
     9721 gctgcgcggg actgcgcgtg gcgagctggc ctgccgatgc ccgttgaacc agacatcgcc
     9781 gacgagggtg cgggcgaagt cggaggcgag gaaggggatc atcccgccga tgtggtcgct
     9841 gtcgatgtgg gtcacgacgg cgacgtcgat ggcggggttg ctggctggca gccggtccag
     9901 gcgggctttg aggagcggcc aggtgtccgg tggcccgccg tcgacgagca tccgccaggg
     9961 tcggcggccc tgccggaggc attcgacgag caggcagtcg ccgagtcggg ccggcagcat
    10021 ctccacgcgg atcgcggtgg ccatggtggc agcgtcctcc ctcgctgcac ggcgccgcaa
    10081 ccgattctgg gaactgccct gtggatgagc gcgaccgggt gtcaggggga cggcatacgg
    10141 tgggtccatg gtcgccgcac tgtccccgag ccgggcctcg gacttcaagc agtgtccgct
    10201 gctctatcgc ttccgggtca tcgaccggct gccgtcgccg acgtccgctg cggcggcgcg
    10261 cggcaccctg gtccacgcgg tgttggagcg gttgttcgac ctgccagccc acgagcggac
    10321 ccccgccgcg gccgccgcgc tggtggcgcc gcaatgggag cggctggtgg ccgaggagcc
    10381 cgagctcgcg ggcctgctgg actccgacga cgacggcgca gccgacgcct ggtatgcgca
    10441 ggcggtcggg ctggtcgagc ggtggttcac cctcgaggac ccgacccggt tggagccggc
    10501 cgagcgcgag ctctatgtcg aggcggaggt ggacggcctg gtcctgcgtg gctatgtcga
    10561 ccgtctcgac gtggcccctg acgggtcgat gcgggtggtc gactacaaga ccgggcgatc
    10621 cccgtcggag ttgttcgagc gttcgtcgct gttccagatg aagttctatg ccctcgtgct
    10681 gtggcggttg cgcggcgtgg tgcctcgtat gctccaactg gtctacctcg gcaacggcga
    10741 ggtagtgcgc tacgagccgg acgaggccga cctgctggcc accgagcgtg gcatcaaggc
    10801 cctgtgggcg gcgatcgagc gcgccgctgc gaccggcgat tggcggcccc gggtgtcgcg
    10861 cctgtgcgac tggtgcgatc acaagtcgct ctgccccgaa tggggtggga ctcccccgcc
    10921 gctgccgacc gacgcacccg ctcgggcgct cgatccggcc gtcaccggtc gggtagtgcc
    10981 ggccgacgac tagtcggtca gctgcgggtc ggtcagctgc gggtcggaag ggccgcgaag
    11041 cccggcagcc cgaccagatc cgttgggctc accccgacca gggtcgggat ctgcaccacg
    11101 ccaggcatcc ggggcaccgg cttcacgctg ggcaccacga tggtctgggc gccggccgcc
    11161 gcggccgagc gggctcccgg ctcggagtcc tcgatggcga tgcactcctc tggcgccagc
    11221 cccagcccgg ctgcacctcg gaggtagggc tcagggtgcg gtttgccgtg ggtgacgtct
    11281 tcgcccgaga cgatcaggtc gaaggcgcca ccgggcaagg cgtcgccgat ggcgtcggtg
    11341 atcgggcggt aggacatcgt caccagcgca gtcgggacgc cggcttcgcg tagcgcggtg
    11401 agcagttccc gcgccccggg gcgccacggc acctctgccc ggatccggcg gacgacgccg
    11461 gtcaccagtc ggtcgacgag ctcatcgatc ggcaggtcca cggggctgtg gtccttgatg
    11521 tatgccgccg agtcggtcag cgcgcgaccc accagagcga aggcatcggc ctcggtccac
    11581 acgccgccga agtcctcgac cagagcgcgt tcctcgagga cccagtaggc ctctgagtcg
    11641 acgagagtgc cgtcgaggtc ccacaacacg gccgctggca ggcgggcaga catcttggtg
    11701 agaccccttc acaggtggtg gtgcgttcgc cgccgccgga gggtgacgag cccaccaagt
    11761 ctaccgaccg cggcgtgttg gtccgccggg tgaggcgcgg cggcataccg gctgggggcc
    11821 gtggccctag ggtggtgtcc tccgattcct gccctgcgag gtgactgtgc ctgtgctccg
    11881 ttccatccca gagttgcgcg agccggtgct cgtcgcggcc ttcgagggct ggaacgacgc
    11941 cggtgaggcc gcgtctgcgg cggccgccca cctcatcgag gtgtgggatg cgcagcaggt
    12001 cggtgagctc gacccggagg actactacga cttccaggtc aatcggccga agatcaccct
    12061 cgtggatggt cagcgtcgga tcacctggcc gacgacgcgg atcttcgtct gccagggcac
    12121 gaccgggccg catgacatcg tcatcgtcga aggcatcgaa ccgtcgatcc gctggcggca
    12181 ctacgccgac gagctcgtag aggtcgccca ggccctgggt gtgcagaccg tcatcaccct
    12241 gggcgcctat ctcgccgatg tcccgcacac ccgggcgatt ccgatcaaca ccagctccga
    12301 cgacgtgagc gtgcaggatc gtttcggcgc tgaggcgagc gaataccaag gcccgaccgg
    12361 gatcgttggg gttctcaccg aagccgtccg ggctgccggg atcgagtcgc tgtcgtgctg
    12421 ggcggccgtg ccgcactacg ccggtggtcc cccgtcgccc aaggccgcgt tggccctgct
    12481 cggccgcctc gaagaggtgc tcgacgtgtc gatccccgcc cgggcgatcg ccgacgaggc
    12541 gaaggcgtgg gagcgcggcg tcgacgagat ggccgagagc gatgaggagg tggccgacta
    12601 cgtgcagtcg ttggaggaag ccaccgacac ggccgaattg cctgaggcca gcggtgatgc
    12661 gatcgcccgc gagttcgagc gttacctacg cggccacgag gacgatccgt cctaactgcg
    12721 ccctagccgt gccctcgccg cgtcctagac gcggactccg aggatgcggt cgacggcacg
    12781 cgcgacgaga ccaggtgccc cgagctcagt tccgccgtgt tccaaggtcc gcacggccca
    12841 cgcgtcgacc agagccagcg caccgggagt gtccagatcg tcggcgactc gctcccggag
    12901 cttggccagc agtccgccga caggcgctcc cccgttgccg gacaatgcgg ttcgccatcc
    12961 cgctagccgc tgggtcgccc ggtgcaggtc ggcgtcaatc cactcccact cggcgcggta
    13021 gtggtgatcc aacagagcca gcctgatcac catcgggtcg acaccggccg cccgcagggc
    13081 cgacaccagc acgaggttgc ccttggactt gctcatcttc tcgccctcga aggccaccat
    13141 cgcctgatgc atatagacct gggcgaacgg ccgcgtcccg gtgagtgcgc agccctggac
    13201 ggcgctcatc tcgtggtggg ggaagaccaa gtcggtccca ccaccctgga cggtgaacgg
    13261 cataccgaga tagcggacgg ccaacgccgt gcactcgatg tgccacccgg ggcgcccgcg
    13321 tccgagcgtg gcgccgtccc ac
//
LOCUS       DAOMZY010000048        13220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_4329_length_13220_cov_6.239499, whole genome shotgun sequence.
ACCESSION   DAOMZY010000048 DAOMZY010000000
VERSION     DAOMZY010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 13220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13220
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4329_length_13220_cov_6.239499"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PLK64_04380"
     CDS             <1..405
                     /locus_tag="PLK64_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79010.1"
                     /translation="DVLVPNAPDAVGTGGKASVAVVTTKVDGVLTVPASAVTGVSTGV
                     GSVGLLKGGVVTATRVQIGAVGGGRVQILSGLSLGDTVVIGDASAPLPTNGGFGGFGG
                     GLGGAGGLTGGAVLVPGPNFGGGAPPGAPPGR"
     gene            complement(427..1002)
                     /locus_tag="PLK64_04385"
     CDS             complement(427..1002)
                     /locus_tag="PLK64_04385"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HPV79011.1"
                     /translation="MSSQAPSPTPFGGLIRKSWQVLLVLSIVEIVLGIVVMAWPGATL
                     RIVGVFFGIFLVVSGISECVVGLSTPLMSGSFRLLNVIAGVLSFILGILCFRDGLGSL
                     AVLGVWVGAGWLMTGFSRLFTFGSLESMPGRNWAIAGAVVTILAGIAAIVYPISSVVT
                     LALLGGIALLVVGIVGLVHAIQWKSTVNAIR"
     gene            complement(1109..2533)
                     /locus_tag="PLK64_04390"
     CDS             complement(1109..2533)
                     /locus_tag="PLK64_04390"
                     /inference="COORDINATES: protein motif:HMM:NF005858.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HPV79012.1"
                     /translation="MTEPLLRGLLDPTRADALVRVADTTLSYAALLTSATAVAEAMAR
                     ESLSRIAIDATPTMDTVISVVGAILAGVEVVPIPADAGPGERAHILNDSGADLWPATA
                     PNGGATEGAAERAAESGRAAYRLPEVSPDAGAFVLYTSGTTGLPKGVRLSRRAVAAGL
                     DALADAWAWTADDVLAHGLPLFHVHGLVLGVLGPLRIGGGLRHVGRPTPSAYAAAAHA
                     GATMFFAVPTIWSRVVAEPESARALRSARLLVSGSAALPVPVFEGLRELTGHEVCERY
                     GMTETLITVATRADGVRRAGCVGVPVAGVKTRVVDDDGAPVPADGTSLGELQVCGPTL
                     FDGYLGLPEATAAAFTADGWFHTGDVATIAADGTHRIVGRASVDLIKSGGYRIGAGEI
                     ESVLLGHPAVAECAVIGVPDDDLGQRIVAVVVPAGPAPDELATKLIDLVVAQLSVHKR
                     PREVRFVAALPRNEMGKVQKTRLT"
     gene            complement(2530..3138)
                     /gene="thpR"
                     /locus_tag="PLK64_04395"
     CDS             complement(2530..3138)
                     /gene="thpR"
                     /locus_tag="PLK64_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HPV79013.1"
                     /translation="MAPTITGWQAGSMGQRMFLAVVPPEPVREDLEAFLDVRPGMRWT
                     HPLHWHVTLAFFASVPEHRLDELIERLATKASKRHTFTTGLAGAGAFPNPARANVLWL
                     GLDCGTDELTHLAASGRAAGAKVGAPPDAQKFRPHLTLARPRPPIEATRWLRVLDSYR
                     SPVWEVVDIALIASYLGEGSGGGSRYETVAQFALGRPGAGTP"
     gene            3137..3646
                     /locus_tag="PLK64_04400"
     CDS             3137..3646
                     /locus_tag="PLK64_04400"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPV79014.1"
                     /translation="MGFPENVLAQGEKVERSLHPHPLTVFGPTLLGVVLIALAVVTSW
                     VTPDDATGNRLQWGAISVLVVLALALVVVPYLRWRTTHYVITSHRVMVRKGILSKSGK
                     DITLSKITDVSFRQTFFDRIIRSGTLSIESAGDSADELLRNIPRSDEIQQLINRLIDE
                     DAKRRSGHS"
     gene            3818..5233
                     /locus_tag="PLK64_04405"
     CDS             3818..5233
                     /locus_tag="PLK64_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPV79015.1"
                     /translation="MEDLPLWLRLAIILLLVAGNAFFVGSEIAISSARRSRIRQLAEE
                     GNSRAKTVKLLHEEPERFYSVTQIGITLVSLGLGAVGMETLHTVVDPWVVAIFTALGD
                     HDALHAAAHTVSYVIGFTVVSFIHIVGGELAPKVLAFHRAEPLSLRVGRLINVMYLTF
                     RPLIAVMKWASDLLLRLSGQGNLGGHGESHFTMSVEEIRMILEASEKDGMLDPEETEM
                     IRGVFELDEQTVRDAMVPRTQIRALEQDATLGDALRYFRDIPHARFPVYVDSLDRITG
                     VVAIKELLTYVAEHSGSDSFSKASKKPVKDFAKPPFLVPDSKPLNDLLADFKRKRQQM
                     AIVIDEYGGTEGIITLEDILEEIVGDYEDEFTRGTRRVKKLTGSAYEIDASMRISDLV
                     DLVSFPFPEDDDYVTLAGLFYKAFGGVPSVGDVVTLESGRLTVLEMDNHRITLVKFED
                     TAHRADGSVGLVESTSAPDPV"
     gene            5269..6474
                     /locus_tag="PLK64_04410"
     CDS             5269..6474
                     /locus_tag="PLK64_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010080005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPV79016.1"
                     /translation="MPSAPVPASPPVRLSARGAVEPFHVMEVIKAASLRQASHGDVIA
                     LCAGQPSTPAPVAARVAAVRALEGEVLGYTEATGIRPLREAIARHYADSYGLAVDPAQ
                     VVVTTGSSAAFTALFLAAFDVGDTVAMSRPGYPAYRNTLQALGARVVEFDCGPDTRFQ
                     PTVAMLEALPEPPAGLVVASPSNPTGTIIDPDELAAIARWCEAHGTLLVSDEIYHGIS
                     FGRQCASAWESSRSAVLVGSLSKYFSMTGWRVGWMVLPPALVRPVEVLIGNLNICPPA
                     VSQAAGVAAFSASAREELDGHVRRYAVNRDLLLRRLPEIGVAKLAPPDGAFYLYFDVS
                     HLTDDSTRWCQEVLDATGVALTPGVDFAPATPGGDPALDGRAFVRLCFAGTTADLDDA
                     MDRLTAYLT"
     gene            6615..8078
                     /locus_tag="PLK64_04415"
     CDS             6615..8078
                     /locus_tag="PLK64_04415"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPV79017.1"
                     /translation="MLPLAAALLATACTGSPTPPGAPTASAAPTGSAGVPGVSGMPPT
                     ELSVAGITVVQSMGNDPLLPVAVRRPVAPGQGSMTDAIAAWANATESSFRADNAPSEQ
                     APAELNVGWQVITASGPVLGIRMETFEFGGASGQTTHRVFYGDGSTTWTGVDLVTTAG
                     RAEVAALVRSAVRASGHATLDPDEVGAAGVDALLTDLTFSAAGDLVLLLDQGTVAPFS
                     AGNLTVTVPQATAEPLLSERGRRVADAVRSGLPYAVSVPTSAAPTPVAPATPVTPAPV
                     PPATPAVAPPPAAAVDCTKLKCVALTFDDGPGSLTGTLLDRLEAAGVVATFFVLGQNV
                     RIHPDLVTRMVRSGMVVGNHTWDHRDMKRLSLDEARSEVDRTSAEIARVTGVSTTLLR
                     PPYGSMSPQTATLGQAIILWDVDTEDWKNRDASITTSRALATVRPGSIILMHDIHAST
                     IEAVPAIIAGLKARGFTLVTVPVLLGNPQPGKVYTRR"
     gene            complement(8063..8740)
                     /locus_tag="PLK64_04420"
     CDS             complement(8063..8740)
                     /locus_tag="PLK64_04420"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HPV79018.1"
                     /translation="MSTSQSPQPGIPLAVPTEEALAPVPAWGVLSAAAAPVVLAAGYL
                     YAASLTPGFQPVSQPLSDLGAANAGTRWLMAVTLMLVGASYIVTALGLRPADIAGRWL
                     LGGAGVMMFWVALIPNNVVGKWTLRHILGSAFVFGLLALWPAMSGHLGPRVPWPLRRR
                     VGVMISIVAFVLIELTLWGIILRADTDGAREVLLYAVTALWPLVVVVWTKVRGAGWAY
                     PAVQRRV"
     gene            8784..10448
                     /locus_tag="PLK64_04425"
     CDS             8784..10448
                     /locus_tag="PLK64_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding dehydrogenase"
                     /protein_id="HPV79019.1"
                     /translation="MPDTDVIVIGAGLAGLVAATEAVAAGKRVVVVDQESRENLGGQA
                     FWSLGGLFLVDTPEQRRMGIKDSHELAWSDWKGTAGWDRLGSGGEDYWGKQWARAYVE
                     FASGEMRAWLSGLDVKFFPLVGWAERGDGTAGGHGNSVPRFHVTWGTGPGICEPFVRL
                     AKEAEVAGRLRYAFRHRVDDLVFTRGVVSGVRGTRLAPTHVARGVATSREPAGEFELS
                     AGAIIVASGGIGGNLDLVRAAWPPRLGLPPGEAVVGVPAHVDGRMVGITARAGGRIVN
                     GDRMWHYVEGVRNWDPVWAQHGIRIIPGPSSVWLDGAGDRLPAPLYPGFDTLGTLEYL
                     RQSGFDHSWFVLNQRIIEKEFVLSGSEQNLDLTNRSVRDVIGRARGGVPASVQAFLDH
                     GEDFVVAPTVRELVAGMNALVPEAPLDPDKVERILRERDLQVDNEFGKDFQIMAIRAA
                     RAFRGDRMTRTVPPHPILDPAAGPLIAVRLSVLTRKTLGGVQTDLAGRVLRMDGTAVP
                     ALYAAGEVAGFGGGGVHGYRALEGTFLGGCLYSGRVAGRSAATALG"
     gene            complement(10671..11777)
                     /locus_tag="PLK64_04430"
     CDS             complement(10671..11777)
                     /locus_tag="PLK64_04430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPV79020.1"
                     /translation="MTNATGHGPDHAGHDHDHGGHGDEHAGHAGHGDEQKHGHDDEQK
                     HGHDEEHGQVGGIRRRFGHGHGHGHGHHDAAVTDDLAMTTAAGIRASIIGLIGLLATA
                     LVQLALVGLTGSVALLSDTVHNLGDCLTALPIWLAFRLARRPPSARFTYGLGRVEDLA
                     GLLVVFAIGASGVYAVFVSIERLLHPSPVEQPWIVVAAGLIGVIGNEAVARFRINAGK
                     RIGSLALVADGEHARSDGLSSLAVVGSGLASLAGWAWADPIVGLVIAALIGALFVRTG
                     RAILARLLDAVDPELVDRVRSAAAEPGGVITVDDVRVRWLGHRARAEIDVTVDSSLSV
                     VEARAVADAVTNEIRQDSPEVKDVAVQILPALTH"
     gene            complement(11774..12130)
                     /locus_tag="PLK64_04435"
     CDS             complement(11774..12130)
                     /locus_tag="PLK64_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPV79021.1"
                     /translation="MNATIPRPEDPSDDVVTLAVEVLRILADSTRLRIATLLIDAELS
                     VSDLAVAVKRPVPGVSQHLAKMRLAHLVSTRKQGTTVLYRIDDEHIRQLVLDTTRHVE
                     HLLSDRPAHHESRPKP"
     gene            12173..12913
                     /locus_tag="PLK64_04440"
     CDS             12173..12913
                     /locus_tag="PLK64_04440"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPV79022.1"
                     /translation="MGVFAKLFAHRGPQAHGHGSHGHGSHGHGAHGHGASGHESSGHA
                     AHGHGVMSHAGSYDRLTRLVTLGRRDRIYAGLAALSGAAPGDHAVDLGCGTGALTRAL
                     AARVGPTGLVTGIDPSEEMVAFASRSATPSLQYAVHPAEALPFDDSSVQVVATALAMH
                     HIDPDQRPAAVAEAHRVLVPGGQLLVVDLQPPRNRVARHVIDALTGPEMANNDLGAVR
                     TLMVDAGFTIVDEGVRSVAFGYVLARKV"
BASE COUNT         2004 a         4550 c         4650 g         2016 t
ORIGIN      
        1 gacgtccttg tccccaacgc ccccgacgcg gtcggcacgg gcggcaaggc atccgtcgcc
       61 gtcgtcacaa ccaaagtgga cggcgtcctc accgtgcccg cgtcggcagt cacgggggtg
      121 tcgaccgggg tcgggtcggt cgggctgctc aagggcggcg tcgtcacggc gacccgggtg
      181 cagatcggtg cggtgggtgg cggaagggtc cagatcctct cgggtctgtc ccttggcgac
      241 accgtcgtca tcggcgatgc gtctgcgccg ttgccgacca acggtgggtt cggcggcttc
      301 ggcggagggt tgggtggtgc gggtggcctc acgggcggtg cggtcctggt gccgggcccc
      361 aacttcggcg gtggggcacc cccaggagcg ccgcccggcc gctgagccga ggcagggatc
      421 agacggtcag cggatggcgt taaccgtgct cttccactgg atggcgtgga cgagcccgac
      481 gattcccacg acgagcaggg cgataccacc gagcagcgcg agggtcacga cggacgagat
      541 cgggtagacg atcgccgcaa tgcccgcgag gatcgtgacg acggcgccgg cgatcgccca
      601 gttgcggccg ggcatgctct cgaggctgcc gaacgtgaag aggcgcgaga agcccgtcat
      661 caaccagccg gcaccgaccc agacgccgag cacggccagg ctgccgagcc catcccggaa
      721 gcagaggatg cccaggatga acgagaggac gccggcgatg acgttgagga ggcggaagct
      781 ccccgacatg agcggggtcg agaggccgac gacgcactcg gagatcccgc tgacgacgag
      841 gaagatcccg aagaagacgc cgacgatgcg cagggtcgct ccgggccagg ccatgacgac
      901 gatgccgaga acgatctcga cgatgctcag cacgagcagg acctgccacg acttacgaat
      961 gaggccaccg aaaggagtgg gagacggtgc ctgggaactc acgatcgatc ctccaatcaa
     1021 cacgcacggg cagccaacgt catcgacgac gcggcgagcc atgtcccttc cattcgaagg
     1081 tacaccgcac ggatcaggct gtcccacctc aggtgagccg ggtcttctgg accttgccca
     1141 tctcgttgcg ggggagggcg gcgacgaaac ggacctcacg cgggcgcttg tgcaccgaca
     1201 actgtgcgac gacgaggtcg atgagtttgg tcgccaactc atcgggcgcc gggcctgcgg
     1261 gcacgacgac cgcgacgatc cgctgcccca ggtcgtcgtc cggcaccccg atcacggcgc
     1321 actcggcgac ggcgggatga ccgaggagga cgctctcgat ctcgcccgct ccgatgcgat
     1381 agccgccgga cttgatgagg tcgaccgacg cccgcccgac gatccggtgg gtgccgtcgg
     1441 cagcgatcgt cgcgacgtcg ccggtgtgga accacccgtc cgcggtgaac gccgcggccg
     1501 tcgcttccgg cagcccgagg tagccgtcga acagcgttgg gccacagacc tgtagctcgc
     1561 cgaggctcgt cccgtcggcc ggcaccggcg ccccgtcgtc gtcgaccacg cgggtcttca
     1621 ccccggccac cggcaccccg acacacccgg ctcgccgcac cccgtcggcc cgggtcgcca
     1681 cggtgatcag cgtctcggtc atgccgtagc gctcacacac ctcgtgcccg gtcaactccc
     1741 gcaggccctc gaacaccggg accggcagcg ccgccgagcc cgacacgagc agccgagccg
     1801 agcgcagtgc gcgggcgctc tccggctcgg cgacgacccg ggaccagatc gtcggcacgg
     1861 cgaagaacat cgtcgcgcct gcgtgggcgg cggcggcata cgcgctgggg gtcggtcggc
     1921 ccacgtgccg caacccgccc ccgatccgca gtgggccgag cacccccagc acgagcccgt
     1981 ggacgtggaa caacggaagc ccgtgcgcga gaacgtcatc ggcggtccag gcccaggcgt
     2041 cggcgagcgc gtcgagcccc gcggccaccg ctcgacgaga cagtcgcacg cccttgggca
     2101 gccccgtcgt ccctgacgtg tagaggacga acgcgcctgc gtcgggggag acttccggca
     2161 gccggtatgc cgccctcccg ctctccgccg cgcgctcggc cgcaccctcc gtcgcgcccc
     2221 cattcggagc cgtcgcgggc cagaggtcgg cccccgagtc gttcaggatg tgggctcgct
     2281 cgccgggccc ggcatcggcc ggaatgggaa cgacctcgac cccggccaag atggcaccga
     2341 ccacgctgat gaccgtgtcc atcgtcggtg tggcgtcaat agctatgcgg gacaacgact
     2401 cccgagccat cgcctcggcg acggccgtgg cggaggtgag cagcgcggca tacgagaggg
     2461 tcgtgtccgc cacccgcacg agagcgtcgg cgcgggtcgg gtcgaggagc ccgcgaagga
     2521 gcggttcggt catggcgtgc ccgcgcccgg ccgaccgagc gcgaactgcg cgacggtctc
     2581 gtagcgggag ccgccacccg acccctcgcc gaggtaggac gcgatgaggg cgatgtcgac
     2641 cacctcccac accgggctcc ggtaggagtc gaggacccgc agccagcggg tggcctcgat
     2701 gggcgggcgc ggtcgggcca gggtgagatg cggcctgaac ttctgcgcgt ccggcggagc
     2761 tccgaccttg gcgcccgcgg cgcgtcccga agccgccagg tgcgtcaatt cgtcggtgcc
     2821 acagtctaat ccgagccaca ggacgttggc gcgggccggg ttggggaagg cgcccgctcc
     2881 tgcgagtccg gtcgtgaagg tatgccgctt gctcgccttc gtcgccagcc gctcgatgag
     2941 ctcgtcgagc cggtgctcag gcaccgacgc gaagaaggcg agggtgacgt gccagtgcag
     3001 ggggtgcgtc cacctcatcc cgggccggac gtcgaggaag gcctcgagat cctcacgaac
     3061 cggctcggga ggcacgacgg cgaggaacat ccgttggccc atgctgccag cctgccaccc
     3121 cgttatcgtg ggggccatgg ggtttcccga gaacgtgctc gcccaaggcg agaaggtcga
     3181 acgcagcctg cacccgcacc cgttgacggt gttcgggccg accctgctcg gggtggtcct
     3241 catcgccctg gctgtcgtca cgtcgtgggt gacgcccgac gatgcgaccg gcaaccgtct
     3301 gcagtggggc gccatctccg tcctggtcgt gctcgccctc gcgttggtcg tcgtgccgta
     3361 tctgcgctgg cgcaccacgc actacgtcat caccagccac cgggtcatgg tgcgcaaggg
     3421 catcctcagc aagtcgggca aggacatcac cttgtcgaag atcaccgatg tgtccttccg
     3481 ccagaccttc ttcgaccgga tcatccggtc aggcaccttg agcatcgagt cggccggcga
     3541 ctcggccgac gagctcctgc gcaacattcc ccgtagcgat gagatccagc agctcatcaa
     3601 ccggctcatc gacgaggacg ccaagcgccg ctccggccac tcctgacggc cgaccgccgg
     3661 tggcggcggc gttccgcacc ggtggcggcg tcggcgcgtc cgctcgcgga ttcgggacgt
     3721 tcatcccccg gtgtagcgcc gttcgcttct cgtacactcg cccacgggtg caggtgtcgg
     3781 tccgcgaccc tgcgaagacc ccgaggagtg gtctttcatg gaggacctgc cgttatggct
     3841 gcggctggcg atcatcctct tgctcgttgc cgggaacgcc ttcttcgtgg ggtcagagat
     3901 cgccatctcc tccgcccgcc gaagtcgcat ccggcaactc gcggaggagg gtaacagccg
     3961 ggccaagacc gtcaagttgc tccacgagga gcccgaacgg ttctactcgg tcacccagat
     4021 cggcatcacc ctcgtctcgc tcgggttggg cgccgtcggc atggagacgc tgcatacggt
     4081 cgtcgacccc tgggtggtgg cgatcttcac cgctttgggc gaccacgacg cgctgcacgc
     4141 cgctgcccac acggtcagct acgtcatcgg gttcaccgtg gtgtcgttca tccacatcgt
     4201 cggcggtgag ttggccccga aggtcctcgc cttccaccgc gccgagccgc tgagcctgcg
     4261 ggtcgggcgc ctcatcaacg tcatgtacct caccttccgt ccgctcatcg cggtgatgaa
     4321 gtgggcctcc gacctgttgc tgcgcctttc gggtcagggc aacctcgggg gccacggcga
     4381 gagccacttc acgatgagcg tcgaggagat ccggatgatc ctcgaggcga gcgagaagga
     4441 cgggatgctc gatcccgagg agaccgagat gatccgcggc gtcttcgagt tggacgagca
     4501 gacggtccgc gacgcgatgg tgccgcgcac ccagatccgc gccctcgaac aagacgccac
     4561 tctcggcgac gccctgcgct acttccgcga catcccgcac gcccggttcc cggtctatgt
     4621 cgacagcctc gaccggatca ccggcgtcgt cgccatcaaa gaactgctca cctacgtcgc
     4681 cgaacacagt ggttccgaca gcttcagcaa ggcgagcaag aagccggtca aggacttcgc
     4741 gaagccgccc ttcctcgtgc ccgacagcaa accgctcaac gacctgctcg ccgacttcaa
     4801 gcgcaagcgc cagcaaatgg ccatcgtcat cgacgaatac ggcggcaccg aggggatcat
     4861 caccctcgag gacatcctcg aagagatcgt cggcgattac gaggacgagt tcacccgggg
     4921 gacgcggcgg gtcaagaagc tcaccggcag tgcctacgag atcgacgcct cgatgcggat
     4981 ttccgacctc gtcgatctgg tgagcttccc gttccccgag gacgacgact acgtcaccct
     5041 cgctggcctg ttctacaagg ccttcggcgg ggtgccgtcg gtcggcgacg tcgtcacgct
     5101 cgaatcggga cggctcaccg tcctggagat ggacaaccac cggatcaccc tcgtcaagtt
     5161 cgaggacacc gcccaccggg ccgacgggtc ggtcggtctc gtcgagagca cctcggcgcc
     5221 ggacccggtc tagggtcacc accgcgacgg tcggctaggt ttttcggtat gccgtccgcc
     5281 cccgtccccg cgtccccgcc ggtccgactg tcggcgcgcg gagcggtcga accgttccat
     5341 gtcatggagg tcatcaaggc agccagcctg cgccaggcca gccacggcga tgtcatcgcg
     5401 ttgtgcgccg gccaaccctc gaccccggct ccggtcgccg ctcgcgtggc agcggtgcgc
     5461 gcgctggaag gtgaggtgct cggctatacc gaagcgaccg gcatacgccc gctgcgtgag
     5521 gcgattgcgc ggcactatgc cgactcctac gggctcgccg tcgaccccgc ccaggtggtc
     5581 gtgacgaccg gctcgtcggc cgccttcacc gcgttgttcc tcgccgcgtt cgatgtcggc
     5641 gacacggtgg cgatgagccg acccggctac ccggcatacc gaaacacgtt gcaggcgttg
     5701 ggtgctcgcg tcgtggagtt cgactgcggt cccgacacac gcttccagcc gaccgtcgcc
     5761 atgctcgagg ccctgcccga gccgcccgcc gggctcgtcg tcgcgagccc gagcaacccg
     5821 acggggacga tcatcgaccc cgacgagctc gccgcgatcg cccgctggtg cgaggcgcac
     5881 gggacgctgc tggtgagcga cgagatctat cacggcatct cgttcgggcg gcagtgcgcc
     5941 agtgcgtggg agtcctcgcg ctcggccgtg ctcgtcgggt cgctgtcgaa gtacttctcg
     6001 atgaccggct ggcgggtcgg ctggatggtc ctgccgccgg ccctggtccg cccggtcgag
     6061 gtgcttatcg gcaacctcaa catctgcccg cccgcggtct cgcaggccgc cggcgtcgcc
     6121 gccttctccg cgtcggcgcg cgaggagctc gacggacacg tgcgccggta tgccgtcaac
     6181 cgtgacctgc tgctgcggcg cctccccgag atcggcgtgg ccaaactggc gccaccggac
     6241 ggcgcgttct acctctactt cgacgtcagc cacctcaccg acgactcgac gcgctggtgt
     6301 caggaggtgc tcgacgcaac gggagtggcc ttgacgccgg gcgtcgactt tgcgccggcg
     6361 acgccgggag gggacccggc cctggatggc cgggccttcg tgcggctgtg tttcgcgggg
     6421 acgaccgcgg atctggacga cgcgatggat cggctcaccg cctacctcac gtaagcgctt
     6481 ccaccgccta gcgtgggctg cgttgcccta tctctccctg atcgactgat gtcgatctcg
     6541 cttgacgaag agctggacag aagatgatgc gcggacggtt cgcgatgacg gttggtcgac
     6601 ggtcgaggcg atgggtgctc cccctggccg ccgcgctgtt ggccaccgcg tgcacggggt
     6661 caccgacccc acccggcgct ccgaccgcgt cagcagcccc gactggcagc gcaggcgtcc
     6721 ccggagtctc aggtatgccg cccaccgagc tctcagtggc ggggatcacc gtggtgcaga
     6781 gcatgggcaa cgacccgttg ctgccggtgg cggtgcgccg ccccgtcgcc ccagggcagg
     6841 ggtcgatgac cgacgcgatc gcggcctggg ccaacgccac cgaatcgagt ttccgggccg
     6901 acaacgcgcc cagtgagcag gcgccggctg agctcaacgt cgggtggcag gtcatcacgg
     6961 cgtccggtcc ggtgctcggc atccggatgg agacgttcga gttcggtggt gccagcgggc
     7021 agaccaccca ccgggtcttc tacggtgacg ggtcgacgac gtggaccggc gtggacctcg
     7081 tgaccacggc ggggcgcgct gaggtcgcgg ctctcgttcg gtcggcggtg cgcgcgtcgg
     7141 gtcacgccac gctcgacccc gacgaggtcg gcgcagccgg ggtcgacgcg ttgctcaccg
     7201 acctcacctt ctcggccgcg ggtgacctgg tgctcctgct ggatcagggc acggtcgcgc
     7261 cgttctctgc ggggaacctc accgtgaccg tcccccaggc gaccgcggag ccgttgttgt
     7321 ccgagcgggg ccgacgggtg gccgacgccg tgcgatccgg cctgccgtat gccgtgtccg
     7381 tcccgacctc ggcggcgccc accccagtcg cgccggctac cccggtcact cctgctccgg
     7441 tgccgccggc cactcccgcc gtggcaccgc cgccggccgc cgctgtcgac tgcaccaagc
     7501 tcaaatgcgt cgcgttgacc ttcgacgacg gcccgggctc actgaccggg accctgctcg
     7561 atcggctcga ggctgccgga gtcgtggcca ccttcttcgt cctgggtcag aacgtgcgga
     7621 tccatcccga cctggtgacc cggatggttc gcagcggcat ggtcgtgggc aaccacacct
     7681 gggaccaccg cgacatgaaa cgactctctc tcgatgaggc gcgctccgag gtcgatcgga
     7741 cctcggccga gatcgctcgg gtcacgggtg tgtccacgac cttgttgcgc ccgccctacg
     7801 gctcgatgtc accgcagacg gccacgcttg ggcaggccat catcctttgg gacgtcgaca
     7861 ccgaggactg gaagaacagg gacgcctcga tcaccacctc ccgggctctg gcaaccgtgc
     7921 gcccggggtc gatcatcctc atgcacgaca ttcacgcgag caccatcgag gccgtgcccg
     7981 ccatcatcgc cgggctcaag gcgcgtgggt tcacgttggt gaccgtcccc gtgttgctgg
     8041 gcaaccccca accgggcaag gtctacaccc ggcgctgaac cgccggatag gcccagcccg
     8101 cgccgcggac cttcgtccac acgacgacca cgagcggcca gagggcggtc acggcataca
     8161 ggagcacctc gcgcgccccg tcggtgtccg cgcgcaggat gatcccccac aaggtgagct
     8221 cgatgagaac gaacgcgacg atcgagatca tcacgccgac ccgccggcgc agcggccacg
     8281 gcacgcgcgg accgaggtgg ccgctcatcg ccggccacaa ggcgagcagc ccgaagacga
     8341 acgccgaccc gaggatgtgc cgcaacgtcc acttcccgac gacgttgttg gggatcaacg
     8401 ccacccagaa catcatcacg ccggccccgc cgagcagcca gcgcccggcg atgtcggccg
     8461 gccgcagccc gagcgccgtg acgatgtagg aggcgccgac gagcatgagc gtgaccgcca
     8521 tgagccagcg ggtccccgcg ttggcggcac cgaggtctga cagcggctgg ctcaccggct
     8581 ggaagccggg ggtgagcgac gcggcataca gataacccgc tgcgagcacg acgggagcgg
     8641 cggccgcgct gagcacgccc cacgcaggga ccggcgccag cgcctcctcg gtcggcactg
     8701 caaggggtat gccgggttgc gggctttgcg aagtgctcac gttcctaccg tagacgccgc
     8761 gcgcgggtgc gtacgatcgg cccatgcccg acaccgacgt catcgtcatc ggagccggcc
     8821 tcgccggcct ggtcgccgcc actgaagccg tcgctgccgg caagcgggtg gtcgtcgtcg
     8881 accaggagtc tcgggagaac ctcggcgggc aggcgttctg gtccttgggc gggctgttcc
     8941 tcgtcgacac ccccgagcaa cgccggatgg ggatcaagga ctcgcacgag ctggcgtggt
     9001 cggactggaa gggcacggcc ggctgggacc ggctggggtc cggcggcgag gactactggg
     9061 gcaagcagtg ggcccgggcc tatgtcgagt tcgcctcggg cgagatgcgc gcctggttgt
     9121 ccgggctgga cgtcaagttc ttccccctcg tcgggtgggc cgagcgcggc gacggcaccg
     9181 cgggcgggca cggcaactcg gtgccgcgtt tccacgtgac gtggggcacc gggccgggga
     9241 tctgcgagcc gttcgtgcgc ctcgccaagg aggccgaggt ggccgggcgg ctgcgctatg
     9301 cgttccggca ccgggtcgac gacctcgtgt tcacccgagg agtcgtcagc ggcgtccgcg
     9361 ggacccggct agcgccgacc cacgtggcgc ggggtgtcgc gacctcgcga gagccggcgg
     9421 gcgagttcga gttgtcggcc ggggcgatca tcgtggccag cggcggcatc ggcggcaacc
     9481 tcgacctcgt gcgggcggcc tggccaccgc gtcttggatt gccacccggc gaggcggtcg
     9541 tcggggtccc cgcccatgtc gacgggcgga tggtgggcat cacggcccgc gccggtgggc
     9601 ggatcgtcaa cggcgaccgg atgtggcact acgtcgaggg ggtccgcaac tgggacccgg
     9661 tgtgggcgca gcacggcata cggatcattc ctgggccgtc gtccgtgtgg ctggacgggg
     9721 ccggcgaccg gctgcccgcg ccgctctacc ccggtttcga cacgctcggc acgctggagt
     9781 atctgcggca gtcggggttc gatcactcct ggttcgtgct caaccagcgg atcatcgaga
     9841 aggagttcgt cctgtcgggc tccgagcaga acctcgacct gaccaatcgg tcggtgcgcg
     9901 acgtcatcgg gcgggcgcgt gggggcgtgc cggcgtcggt gcaggcgttc ctcgatcacg
     9961 gcgaggattt cgtcgtggcg ccaaccgtgc gcgagctcgt tgccgggatg aacgccttgg
    10021 ttcctgaggc gccgctggac cccgacaagg tggagcggat cctgcgtgag cgcgatctgc
    10081 aggtggacaa cgagttcggc aaggacttcc agatcatggc gatccgggcg gcgcgggcct
    10141 tccggggcga ccggatgacc cggacggtgc cgccgcatcc gatcctcgac ccggcggctg
    10201 ggccgctcat cgcggtgcgg ctgtcggtgc tgacccgcaa gacgctcggg ggagtgcaga
    10261 ccgacttggc ggggcgggtg ctgcggatgg acggcacggc ggtccctgcc ctgtatgccg
    10321 cgggagaggt tgccgggttc ggcggcggtg gggtgcatgg gtaccgggcg ttggagggca
    10381 ccttcctcgg cgggtgcctg tattccgggc gagttgccgg gcggtcggcg gcgacggcgc
    10441 tgggctgagc gctgaggtcg gcggtcggcg gtcggcggca ccccagtgat cgcacaccgt
    10501 ggcgcgctct gatcccggct caggtctgga ttgggctgcg ttcgtcgtgc gtgcgcacgg
    10561 agtgcgtgac ggagtccgtt tgatggggtg atcgccccct tgaacgcaca ccgtcacgca
    10621 cccagccggg catcgaccca accatcagga gcgctgaggc tggcccgggg ctagtgcgtc
    10681 agcgcgggga ggatttggac ggcaacgtct ttgacctcgg gggagtcctg gcggatctca
    10741 ttggtcaccg catccgcgac cgcccgggcc tcgaccacgg acagcgaact gtcgactgtc
    10801 acgtcgatct cggcccgtgc acgatggcca agccaacgga cgcggacgtc gtcgaccgtg
    10861 atgacaccgc ccggttcggc ggcggcggat cggactcggt ccacgagctc gggatcgacg
    10921 gcgtcgagga gtcgggccag gatcgctcgt ccggtccgga cgaacagggc gccgatgagg
    10981 gccgcgatga cgagcccgac gatcgggtcg gcccaggccc agccggccag gctggccaac
    11041 ccggaaccta cgaccgccag cgaggagagg ccgtcggagc gggcatgctc accgtcagcg
    11101 acgagcgcca gtgaaccgat ccgcttgccg gcgttgatcc ggaaccgggc gacggcctcg
    11161 ttcccgatga ccccgatgag ccccgcggcc acgacgatcc acggctgctc caccggcgag
    11221 gggtgcagga gccgctcgat cgagacgaac acggcataca ccccggaggc gccgatcgcg
    11281 aagaccacca gcaaccccgc gaggtcctca acccggccga gaccgtaggt gaaccgggcc
    11341 gagggagggc gccgagcgag gcggaaggcg agccagatgg gcagggccgt gaggcagtcg
    11401 ccgaggttgt gaaccgtgtc ggagaggagc gcgaccgatc cggtgagccc aacgagggcg
    11461 agttgcacga gagcagtggc gagcaggccg atcaagccga tgatcgaggc gcgaatgccc
    11521 gcagcggtcg tcatggccag gtcgtcggtc actgcggcgt cgtgatgccc gtgaccatgg
    11581 ccgtgcccgt gcccgaatcg ccgacggatg ccgccgacct gcccgtgctc ttcgtcgtgc
    11641 ccgtgcttct gctcgtcgtc gtgcccgtgc ttctgctcgt cgccgtgccc ggcgtgcccg
    11701 gcgtgctcgt cgccgtgacc gccgtggtcg tggtcgtgac ccgcgtggtc aggcccgtgg
    11761 cccgtggcgt tggtcatggt ttgggcctcg attcgtggtg ggcaggacgg tcgctcagca
    11821 ggtgctcgac gtggcgagtg gtgtcgagca cgagttgccg aatgtgctcg tcgtcgatcc
    11881 gatagagcac cgtcgtcccc tgcttgcgcg tggacaccag gtgtgcgagc cgcatcttcg
    11941 ccaggtgctg cgagacgccg gggaccggcc gcttgaccgc gacggcgagg tcgctcaccg
    12001 agagttccgc gtcgatgagg agcgtggcga tccgtagccg agtcgagtcg gccaggatgc
    12061 gcaacacctc gaccgcgagc gtgacgacat cgtcgcttgg atcctcaggt cgaggtatgg
    12121 ttgcattcat acgcaaatag tagagcaacc tcggcgtaga gaaggagtcg gcatgggcgt
    12181 gttcgcgaaa ctgttcgcac accgcggtcc acaggcccac gggcacgggt cacacgggca
    12241 cgggtcgcac gggcatgggg cgcacggcca tggggcgagt gggcacgagt cgtcggggca
    12301 tgcggcgcac gggcacggtg tcatgtcgca cgcggggagc tacgaccggc tcacccggtt
    12361 ggtgactctc ggccgtcgcg accgcatcta cgccggcctg gccgccctca gtggtgctgc
    12421 tcccggcgat cacgcggtcg acctcggctg cggcaccggc gcactgaccc gagcgctcgc
    12481 ggcacgggtc gggccgaccg gcttggtcac cgggatcgac ccttccgagg agatggtcgc
    12541 cttcgcctcg cgcagcgcca ccccgtcgct gcagtatgcc gtgcaccccg ccgaggctct
    12601 cccgtttgat gactcgagcg ttcaggtcgt ggcgaccgcc ctggcgatgc accacatcga
    12661 tcccgatcag cgtccagcag cagtggccga ggcccatcgg gtgttggtgc cgggtgggca
    12721 gttgctcgtc gtcgacctac agcccccgcg aaaccgggtt gcccgtcacg tcatcgacgc
    12781 cctcaccggc cccgaaatgg cgaacaacga tctcggggcc gttcgcacgc tgatggtcga
    12841 tgccgggttc accattgttg acgaaggcgt tcgctcggtc gctttcggtt acgtcctggc
    12901 ccgcaaggtc tgacagtcag tcgcgtggct ctgcctggcc ggccccctgc cgagaattca
    12961 tcggcgagtg tgagggattc gggggaatct gagcccgggg tgccggaaaa caccggcgag
    13021 tgtgtggttc ttgggcgacg gtgcttgcgt catgccagac gcgcggcata cgtcgcgttt
    13081 ggcatgacgc acgtcaccac tccgcgaaat catcggcggg tgtgaggatc tggggcgcga
    13141 ccggcatacc cttcgcccac gtcaccggcg ggtgtgagag attcggggga atctgagccc
    13201 ggggtgccgg aaaacatcgg
//
LOCUS       DAOMZY010000049        13068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_4393_length_13068_cov_5.623684, whole genome shotgun sequence.
ACCESSION   DAOMZY010000049 DAOMZY010000000
VERSION     DAOMZY010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 13068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13068
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4393_length_13068_cov_5.623684"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(307..990)
                     /locus_tag="PLK64_04445"
     CDS             complement(307..990)
                     /locus_tag="PLK64_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPV79023.1"
                     /translation="MSVSGVGVSGRGRLVVFDCDGVLVDSERLNVALDVEAIGELGWA
                     ITREEVIARHVGLSEADATADIESVIGRPVPAEWVLRWRSAYHAAYETQLEAVPGVGA
                     AIADLVADGWAVCVATSGERATTWRKLARCGLAQWFTEATVFTAADVARGKPAPDLFL
                     WAGAQLGVAPERCVVVEDSPHGVAAARAAGMPCVGFAGSVTSRGQLVDADVVIDDMSA
                     LVAAICDLV"
     gene            complement(997..4917)
                     /gene="purL"
                     /locus_tag="PLK64_04450"
                     /gene_synonym="purI"
     CDS             complement(997..4917)
                     /gene="purL"
                     /locus_tag="PLK64_04450"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009760541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HPV79024.1"
                     /translation="MGASPAPVLTSFEGGRALSDFRAVGLLRRLRDRVPTITGVTARH
                     VHWVASATPLDADTHTRVAQLLNYGDPYAAPAPDAGGAGDAPVTVLVVAPRLGTVSPW
                     ASKATDIAHNCGFEIRRVERVTEFRLEGVALAGDDLAAAAEVLHDRMTESVLLGHDSA
                     AALFAEREAEPLAHVAVLERGREALVEANATYGLALSDDEVDYLVEAFRGLGRDPSDV
                     ELMMFAQANSEHCRHKIFNARFVVDGEVQDRSLFQMIRNTESVSPRHTVVAYSDNASV
                     MEGGVVPRWLPTEQCEPSRYAAHEDTVHVLMKVETHNHPTAISPFPGAATGAGGEIRD
                     EGATGRGSRPKAGLTGFAVSNLHLPGTDEPWERDPIGRPGHLATPLEIMLDGPIGGAA
                     FNNEFGRPGLGGFFRVYEQTVAGVRRGFHKPIMSAGGLGTISASQTHKVAFPAGTLLV
                     QLGGPGMRIGMGGGAASSMAAGANAAHLDFDSVQRGNPEIERRAQEVVNHCWALGEDN
                     PILAIHDVGAGGLSNAFPELVDGAGLGAHFDLRAIPLEETGLAPKEAWCNESQERYVV
                     ALAPESLALFAQLCERERCPFAVVGVASDDGMLVLADGPDGERAIDMPMEVLLGKPPQ
                     MQRDVERVARTEPELSLEGIDLGVAAFDVLRHPTVASKRFLVTIGDRTVGGLSHRDQM
                     VGPWQVPVGDVAVTLAAYDGFAGEAMASGERMPLAAIDAPASGRMAVGEALTNLLAAP
                     AELERIKLSCNWMAACGEPGEDAALFDTVAAVAMELCPALGVGVPVGKDSLSMRTTWT
                     DESGQGRKVVAPVSLVVTAFAALPDVRGTLTPQLRGGGSDLVLVDLGAGQNRLGGSIL
                     GQVRGGFGGAVPDLDDPARMRALYAAMADLRERDLVLAYHDRSDGGLWATVSEMAFAG
                     RVGVQLGLPAGLSDADVVRALFAEELGVVLEVPRASLGDVVALLREHGLGDCVQVVGR
                     TMKTSAVAVTRGDEVLLHKEIHELQQAWDDVSWRIAALRDNPECADEEHAAAGHPDDA
                     GLQVALSFDAAVDVAAPYVSLGTRPKVAILREQGVNSHVETSYAFDRAGFDTYDVHMT
                     DLQSGRADLAAYQGFVACGGFSYGDTLGAGEGWARSVLFNPALAESFHEFFHRPDTFA
                     LGICNGCQMMAALADHIPGAEAWPRFTRNRSEQFEGRLSLVEVLDSPSIFFSGMAGSR
                     IPIAVAHGEGLADFSQRGDAAAVHEAMRFVDGAGRPATAYPANPNGSPGGLTAVTTPD
                     GRFTVLMPHPERVLRNAQMSWTSGSVDEMSPWLRMFRNARAWVG"
     gene            complement(5029..6105)
                     /locus_tag="PLK64_04455"
     CDS             complement(5029..6105)
                     /locus_tag="PLK64_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPV79025.1"
                     /translation="MTDVAGQGVTLPAPRTPDPQQAPPLRWGLLGAGWIADKFVTSLR
                     AHTTQIPYAVWAPRPLAAAAFAQAHGIPHVHSSVADLLAAPDVDVVYVAGPHHSHHEL
                     TLAAIAAGKPVVVEKPLALNAAQAREIAAAASAAGVFVMEALWSVCLPKFDVVRQVLD
                     AGVLGRIHTVTADIGEYFDRDHRILRRDLAGGPVLDLGTYPVAFATWILGAPSEVRAL
                     AQPDPSGVLSQTAAILRYDEGDHAGALALVHASLATATPTAGEISGDLGTLSLDPPFY
                     QPGGVTLRLRDGCAARYDEPRIAHEGLYFEAVEAARCIAAGLTQSPLRPLADSIAMLA
                     VMDEVRRQTGDVFDMEAGGIPDPA"
     gene            complement(6102..7097)
                     /locus_tag="PLK64_04460"
     CDS             complement(6102..7097)
                     /locus_tag="PLK64_04460"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV79026.1"
                     /translation="MSTTAAAETRTKFDRGAFVRQYAIVGILVLFVLGLSLATGGKFL
                     QAQNLLNVVTQVAAIGIIAIGMTFVIITLGIDLSVGSLLAVAAVVATSLAQIPSDTAK
                     FPNMSFSLIIPIVAGLAAGAIGGFTNGFLISVFKIAPFIATLGMMSAARGLALIYSDG
                     RPISRLDPGFNFLGQGSIIGIPVPIIIFAVVAVAAHVVLNYTRFGRHVYAIGGNEQAA
                     RVSGINIKRATIIIYTLCGLLAGIAGIISAGRIGSGNPQLGTGIELDAITAAVIGGTS
                     FKGGIGTIWGTVIGALIIQSLNTGLVLLGMNDFVQMVVKGAIIIIAVILDERKNR"
     gene            complement(7109..8632)
                     /locus_tag="PLK64_04465"
     CDS             complement(7109..8632)
                     /locus_tag="PLK64_04465"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPV79027.1"
                     /translation="MSDEFLLQMQDVSKTFPGVRALAGVNLEVRPGTVHALMGENGAG
                     KSTLMKILIGMYQADPGATIILGGEKVDIPDTAAGLGLGISMIHQELSPVPEMTVAEN
                     IFLGREPVARFGLVSRKQMVSSCRELFQRWEIDIDPRARMRTLSIAQTQMVEIAKAIS
                     FDSRIIIMDEPTSAITEREVEHLHRMIRQLKSTGASIIYITHKMDEVFNISDYVTVFR
                     DGQLVKTLPASELDRDKLITLMVGRELTEMFPKEFVEIGEVVLSVRNLHRGSVVKDVS
                     FDLRRGEILGFAGLMGAGRTEVLETLFGVVAAESGEIVVDGKSVRIRQPKDAIDAGLA
                     LLTEDRKLTGIMGVLSVRDNMIMAALPRYSPGGMLQKALIEEVCQDQRKALALKTPSL
                     NQHIQNLSGGNQQKVLISRWLLTLPEILMIDEPTRGIDVGAKSEIHRLMSALAKTGKA
                     IIMVSSEMPEVLGMSDRVVVMHDGKVSGIVDRADATQERIMQLATGPAGVTGPADAA"
     gene            complement(8765..9799)
                     /locus_tag="PLK64_04470"
     CDS             complement(8765..9799)
                     /locus_tag="PLK64_04470"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPV79028.1"
                     /translation="MAISHGLKVGALLLATTSLFAACSSTAGTTAATTAAATKAAAAT
                     TTTPAAAGSSAVVKPKDGKALKIGVTFPILDQFLQKVADGISARAAEAGVEVNIQASQ
                     EKTDVQLAQVETFIANKVDAIIVVPVDTDAAGPMTAKAQAAGIPLIYVNRKPADLPAG
                     VPYVGSDSLVSGNLEMTQLAKLANGKGNVVILQGDPSNEAAVLRTKGCVDVVAKNPDM
                     KVIKTQAGNWQREKGQSIMETWLQSGDQIDVVCANNDEMALGAINALKAAGKLDKTIV
                     GGVDATGDALAAMKAGELKVTVFQDAKGQGAGGVDAAIKLYNKDTVPAYVDVPYVLVT
                     PENMAQFAGK"
     gene            complement(10232..11236)
                     /locus_tag="PLK64_04475"
     CDS             complement(10232..11236)
                     /locus_tag="PLK64_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPV79029.1"
                     /translation="MTLRIGMIGPGGMGQAHIERIHTVIAGGRVVAVSDVNAATAQAV
                     ADRIGATAYATSAELIASPDVDAVMICSHGPAHEPDVLACIAAGKPVFCEKPLTPTAD
                     AALRIMEAEQAGGRRLVTVGFMRRFDASYREMKAILDSGDLGEALMVHCAHRNPTVPD
                     FYTWDMAINDTAIHEIDTMRWLLGEEFVSARVDKPKKTSLRFEHLQDPLVLVLETASG
                     VRVDDEVFVNCQYGYDIRCELVAEKGAVSLADQNVVTRRDSVGRNNRISMNHNERFWG
                     AFVTEVQEWITAAAKGEHSGSTAWDGYAAACVCDAGVKALTDDGVVPVQMIAKPDFYA
                     "
     gene            complement(11258..12190)
                     /locus_tag="PLK64_04480"
     CDS             complement(11258..12190)
                     /locus_tag="PLK64_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPV79030.1"
                     /translation="MAESKARNTDPRYSKLTIGVCPDQWGVWFPQDDKQIDWDVALKE
                     MQTAGFSVMETGPFGYFPKDPTQLRDVMDEHGFTVVAGTGWGILHKAEAWNDTETFFR
                     AIGETHAAVGAKFVVHLPPMFRDEKSGAYTDDRTLSTEAWNLYIKNADKLGRIMKEDY
                     GLQMVLHPHGDSHIETPEDIDRIFQATDPEYVGFCLDTGHIVYGGGDPMELCRKYPER
                     ISYVHIKAMDQALVAQAHDEDWPFVKAVHAGCSVAPPAGLPDMGQLIEALADLDKELY
                     VVCEQDMYGCAKDYPFPNAVTTREYLASLGLGLK"
     gene            complement(12398..>13068)
                     /locus_tag="PLK64_04485"
     CDS             complement(12398..>13068)
                     /locus_tag="PLK64_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPV79031.1"
                     /translation="GSAGERCMAISVLLAVDSIADELIAKISERMATLKTGDGSMGCD
                     MGPIVTGVARDRIVGYIEAGVGAGAELVVDGRETEFHGGADGFFVGPTLFDKVSTDMS
                     IYTDEIFGPVLSVVRVESYDEGLELINSSLYGNGTAIFTNDGGAARRFQNEVQVGMIG
                     INVPIPVPMAYYSFGGWKASLFGDSHAHGMEGVHFYTRTKAVTQRWLDPSHGGLNLGF
                     PQNK"
BASE COUNT         2072 a         4476 c         4437 g         2083 t
ORIGIN      
        1 ccgcgcctcc cccctccggc cgggaaactc gacactcggt gagttccctc cgagcaccag
       61 cccgaccccg gccgggaaac tcgacactcg gtgagttccc tccgagcacc agcccgaccc
      121 cggccggaaa gtcgacactc gctgacttgc ctgcccaccc tgcctgcctt cgtgtcaaaa
      181 ggtcgacgat ctttcgccag gtcgaccgaa tttcgtcgag caacgaagac gtcgtcgacc
      241 tttcgaacgg cgatgtggcg cccggcccaa gccatccatc cagccagtcg gccagcgcag
      301 ggttagctag accaggtcgc agatcgcggc gaccaacgcc gacatgtcgt cgatgacgac
      361 gtcggcatca accaactgcc cccgcgaggt gacgctgccc gcgaagccga cgcacggcat
      421 accggcggct cgtgcggcgg ccaccccgtg cggcgagtct tcgacgacga cgcaccgctc
      481 gggcgcgacg cccaactgcg cgcccgccca gaggaagagg tcgggcgccg gcttcccgcg
      541 cgccacgtcg gcggcggtga agacggtcgc ctcggtgaac cactgcgcca gcccgcaccg
      601 ggcgagcttg cgccacgtcg tcgcgcgctc gccgctcgtc gcgacacaca ccgcccaacc
      661 gtcggccacg aggtcggcga tcgccgcgcc taccccgggc acggcctcca gctgcgtctc
      721 gtatgccgcg tgataggcac ttcgccagcg cagcacccac tcggcgggca cgggccggcc
      781 gatgaccgac tcgatatcgg ccgtcgcgtc ggcctccgac agcccgacgt gcctggcaat
      841 gacttcctcg cgcgtgatcg cccagcccaa ctcaccgatc gcttccacgt cgagcgcgac
      901 gttgagccgt tcgctgtcga ccagcacgcc gtcgcagtcg aagacgacca gccgccccct
      961 gcccgacacg cccacgcccg acacgctcat cggcgctcag cccacccaag ccctcgcgtt
     1021 gcggaacatc cgcagccacg ggctcatctc gtcgaccgag ccgctcgtcc atgacatctg
     1081 cgcgttgcgt agcacccgct ccgggtgcgg catgagcacg gtgaagcgcc cgtccggggt
     1141 cgtcacggcc gtcagcccac cagggctgcc gttgggattc gcggggtatg ccgtcgccgg
     1201 cctcccagcc ccgtcgacga accgcatcgc ctcgtgcacc gccgccgcat cgccccgctg
     1261 cgagaagtcg gccagcccct caccatgagc gactgcgatc gggatgcggc tgccggccat
     1321 ccccgagaag aagatcgacg ggctgtcgag cacctcgacg agtgacagcc gcccctcgaa
     1381 ctgctcactc cggttgcggg tgaaccgcgg ccacgcttcc gctcccggga tgtgatcggc
     1441 aagggcagcc atcatctggc agccgttgca gatccccaac gcgaaggtgt ccggccggtg
     1501 gaagaactcg tggaacgatt ctgcgagagc aggattgaag agcaccgacc gcgcccagcc
     1561 ctcgcccgcc cccaacgtgt cgccgtagga gaagccgcca cacgcgacga acccctggta
     1621 tgccgcgagg tcggctcgcc cgctctgcag gtcggtcatg tggacgtcgt aggtgtcgaa
     1681 gcccgcccgg tcgaaggcgt agctcgtctc gacgtgcgag ttgacgccct gctcacgcag
     1741 gatcgcgacc ttgggccggg tgccgagcga cacgtagggc gctgccacgt caacagcagc
     1801 atcgaagctc aaggccacct gaagtccagc gtcgtccggg tgaccggcag ccgcgtgctc
     1861 ctcatcggcg cattcggggt tgtcgcgcag cgcggcgatc cgccacgaga cgtcgtccca
     1921 ggcttgctgg agctcgtgaa tctccttgtg cagcaacact tcatcgcccc gcgtcaccgc
     1981 aacggcggac gtcttcatag tgcgacccac gacctgcacg cagtcgccga gcccgtgctc
     2041 gcgcagcagg gcgacgacgt cgcccaacga agccctcggc acttcgagca caacaccgag
     2101 ttcctcggcg aagagggcgc gcacgacatc ggcatccgac aggccggccg gcagcccgag
     2161 ttgcacaccg acccgaccgg caaacgccat ctcggacacc gtcgcccaca ggccgccgtc
     2221 ggaacggtcg tgataggcca agacgagatc gcgctcgcgc aggtcggcca tcgcggcata
     2281 caaggcgcgc atccgggccg ggtcgtcgag gtcagggacc gctcccccga agccgccgcg
     2341 cacctggccg aggatcgacc cgccgagacg gttctggccc gcgccgaggt cgacgagcac
     2401 gaggtccgaa cccccgcctc gcaactgcgg cgtgagcgtg ccgcgcacgt cgggcagcgc
     2461 cgcgaacgcc gtgacgacca ggctgaccgg cgcgacgacc ttgcgcccct gccccgactc
     2521 gtcggtccac gtcgtgcgca tcgacagcga gtccttgccg actggcacgc cgacccccaa
     2581 cgcggggcat agctccatcg ccaccgctgc caccgtgtcg aacagcgccg cgtcttcgcc
     2641 gggctctccg caggccgcca tccagttgca ggacaacttg attcgctcaa gctcggcggg
     2701 cgcagccagc aggttggtca gcgcctcgcc gaccgccatc cgcccggagg cgggggcgtc
     2761 gatcgccgca aggggcatcc gctcgccgct ggccatcgcc tcgcccgcaa agccgtcgta
     2821 tgccgcgagc gtcaccgcga cgtcgcccac gggcacctgc cacggcccca ccatctggtc
     2881 gcggtggctc aaccccccga cggtccggtc gccgatggtc acaaggaaac gcttgctggc
     2941 caccgtcggg tggcgcagga cgtcgaaagc agccaccccg aggtcgatcc cctcaaggct
     3001 caactcgggc tcggtgcgag ccacccgctc gacgtcgcgc tgcatctgcg gcggcttgcc
     3061 gagcagaacc tccatcggca tgtcgatggc ccgctcgccg tcggggccgt ccgccaggac
     3121 gagcatcccg tcgtcactgg ccactccgac gacggcgaag gggcaccgct cgcgttcgca
     3181 caactgggcg aagagtgcca gcgactccgg agccagggcc acgacatagc gctcctgact
     3241 ctcgttgcac cacgcctcct tcggcgcaag cccggtctcc tccaacggaa ttgcccgcag
     3301 atcgaagtgc gcacccagac ccgcaccatc gaccaactcg gggaaggcgt tggacagccc
     3361 gcccgcaccg acgtcgtgga tcgccaggat cgggttgtcc tcgccgagcg cccaacagtg
     3421 gttgacgacc tcctgggccc gccgctcgat ctcggggttg ccgcgctgca ccgagtcgaa
     3481 gtcgaggtgc gcagcgttgg caccggccgc catcgacgac gccgcgccgc cacccatgcc
     3541 gatccgcatc cccggcccac cgagttgcac gagcagcgtc ccggcaggga aggccacctt
     3601 gtgcgtctgc gacgctgaga tcgtcccgag gccaccggcg ctcatgatcg gcttgtggaa
     3661 gccccggcgc acgcccgcca ccgtctgctc ataaacccgg aagaagccgc ccagccccgg
     3721 ccgcccgaac tcgttgttga atgccgcgcc gccaatcggc ccgtcgagca tgatctccag
     3781 cggcgtcgcc aagtgccccg gccgaccgat cgggtcgcgc tcccacggct cgtcggtgcc
     3841 gggcaggtgc aggttcgaca cggcgaagcc ggtgagcccg gccttgggtc gcgacccccg
     3901 cccggtcgcg ccctcgtcgc ggatctcccc gcccgcgccg gtcgccgcac cagggaaggg
     3961 cgagatggcc gtcgggtggt tgtgcgtctc gaccttcatg aggacgtgca cggtgtcctc
     4021 gtgcgcggca taccggctgg gctcgcactg ctcggtcggg agccagcgtg ggacgacccc
     4081 gccctccatc accgaggcgt tgtcggagta ggcgaccacg gtatgccgcg ggctcaccga
     4141 ctcggtgtta cggatcatct ggaagagcga gcggtcctgc acctcgccgt cgacgacgaa
     4201 ccgggcgttg aagatcttgt gccgacagtg ctcgctgttt gcctgggcga acatcatcaa
     4261 ctcgacgtcg ctggggtcac gcccgagccc gcggaacgcc tcgacgaggt agtcgacctc
     4321 gtcgtcggac agggccagcc cgtaagtcgc gttggcctcg acgagcgcct cgcgcccccg
     4381 ctcgaggacg gcgacgtgcg ccagcggctc agcctcacgc tcggcgaata gcgctgcggc
     4441 actgtcgtgt ccgagcagca cgctctcggt catccggtcg tgcaacacct ccgcagcggc
     4501 ggccaggtcg tcgccagcaa gggcgacacc ctccagccgg aactcggtca cccgctcgac
     4561 ccggcggatc tcgaacccgc agttgtgggc gatgtcggtc gccttggacg cccacggcga
     4621 gaccgtcccg aggcggggcg cgacgacgag gacggtgacc ggggcgtcgc ccgcaccgcc
     4681 ggcgtcgggg gccggggcgg catacgggtc gccatagttg agcaactgcg cgacgcgcgt
     4741 gtgcgtgtcg gcgtcgagag gggtcgcact ggcgacccaa tggacgtgtc gggcggtcac
     4801 cccggtgatg gtggggaccc ggtcgcgcag gcgccgcagc agccccaccg cccggaagtc
     4861 cgagagggcg cgtccgccct cgaagctcgt tagcaccggg gcgggcgagg ctcccatgac
     4921 aggcggactc ctcaacaaag ggtgggcgcg ccgggtcggc gacggctcac gcagagccta
     4981 ccgggctgac ggggcggctc ccccgggagt ctcggcggct acgtgcggtc aggcgggatc
     5041 gggtatgccg cccgcctcca tgtcgaacac gtcgccggtc tggcggcgca cctcgtccat
     5101 aacggcgagc atggcgatcg agtcggccag cggccgcagc ggcgactgcg tcagcccagc
     5161 cgcgatgcat cgggcagcct cgacggcctc gaagtacagc ccctcgtggg cgatccgggg
     5221 ctcgtcgtat cgagcggcac agccatcccg cagccgcagg gtcaccccgc ccggctgata
     5281 gaagggcggg tcgagagaga gcgtgcccaa gtcgccgctg atctccccgg ccgtgggcgt
     5341 cgcggtcgcc agcgatgcgt gcacgagggc gagcgcaccg gcgtggtcgc cctcgtcata
     5401 ccgcaggatc gcggccgtct ggctgagcac gccgctggga tcgggctgcg cgagggcgcg
     5461 gacctcgctc ggcgcgccga ggatccacgt ggcgaaggcg accggatagg tccccaggtc
     5521 gaggaccggg cccccggcga ggtcacgccg caggatccga tggtcgcggt cgaagtactc
     5581 cccgatgtcg gccgtgacgg tgtggatgcg accgaggacg cctgcgtcga gcacctgccg
     5641 gacgacgtcg aacttgggca ggcacacgct ccagagcgcc tccatgacga agacgcccgc
     5701 ggccgaggcg gcagcggcga tctcgcgagc ctgggcggca ttgagcgcca gcggcttctc
     5761 gacgacgacc ggcttgcctg cggcgatggc cgccagcgtg agctcgtgat gcgagtggtg
     5821 ggggccggcc acgtagacca cgtcgacatc gggggcggcc aacaggtccg cgacgctcga
     5881 gtggacatgg ggtatgccgt gcgcctgcgc gaacgcggcg gcggcgagcg ggcgcggtgc
     5941 ccacacggca tacgggatct gcgtggtgtg cgcgcgaagg gacgtgacga acttgtcggc
     6001 gatccacccc gcgcccagca ggccccaccg aagcggtggg gcctgctggg ggtcgggcgt
     6061 gcgcggcgcc ggcagcgtca caccctggcc ggcgacgtcg gtcaccggtt cttacgctcg
     6121 tcgaggatga cggcgatgat gatgatggcg cccttgacga ccatctggac gaagtcgttc
     6181 atgccgagca gcacgaggcc ggtgttgagg ctctggatga tcagcgcgcc gatcaccgtg
     6241 ccccagatcg tgccgatgcc gcccttgaag ctcgtgccac cgatgaccgc cgcggtgatc
     6301 gcgtcgagct cgatgccggt gccgagctgc gggttgcccg agccgatgcg gccggccgag
     6361 atgatgccgg cgatcccggc gagcagacca cagagggtgt agatgatgat cgtcgcgcgc
     6421 ttgatgttga tgcccgagac acgggctgcc tgctcgttgc cgccgatggc gtagacgtgc
     6481 cgaccgaagc gcgtgtagtt gagcacgacg tgagccgcaa cggcgaccac ggcgaagatg
     6541 atgatcggga cggggatgcc gatgatcgag ccctggccga ggaagttgaa gcccgggtcc
     6601 aaacgcgaga tcggtcggcc gtcgctgtag atgagggcga ggccgcgggc cgcgctcatc
     6661 atgccgaggg tggcgatgaa cggagcgatc ttgaacaccg agatgaggaa gccgttggtg
     6721 aacccgccga tcgctccggc cgccagacca gcgacgatcg gaatgatgag cgagaacgac
     6781 atgttcggga atttggctgt gtccgaaggt atctgagcca aactcgtcgc gacgaccgcc
     6841 gctaccgcca gcagggaccc gacggacagg tcgatgccca gggtgatgat gacgaaggtc
     6901 atgccgatgg cgataatccc gatcgccgcg acctgagtca cgacgttgag caggttctgc
     6961 gcctgcagga acttgccgcc ggtggcgagg gacagaccga ggacgaagag cacgaggatg
     7021 ccgacgatgg cgtactgccg gacgaaggca ccgcggtcga acttggtcct ggtttcagct
     7081 gctgctgtgg tggacatcga gttacctctc aggctgcgtc agcggggccg gtgacgcccg
     7141 cgggtccggt ggcgagctgc atgatgcgct cctgggtggc gtcggcccgg tcgacgatgc
     7201 cgctgacctt gccgtcatgc atgacgacga cgcggtcgct catgccaagc acctcgggca
     7261 tctccgagga gaccatgatg atggccttgc cggtcttggc cagggccgac atgaggcggt
     7321 ggatctcgga cttggcaccc acgtcgatgc cacgggtcgg ttcgtcgatc atgaggatct
     7381 ccggcagcgt gaggagccat cgcgagatga gcaccttctg ctgattgcca cccgacaggt
     7441 tctggatgtg ctggttgagc gagggggtct tgagggctag ggccttgcgc tggtcctggc
     7501 agacctcctc gatcagggcc ttctgcagca tgccgccggg gctatatctc ggcagggcgg
     7561 ccatgatcat gttgtcgcgg accgacagca cacccatgat cccggtgagt ttgcggtcct
     7621 cggtgagcag ggccaggccg gcgtcgatcg cgtccttggg ctgacggatc cggacggact
     7681 tgccgtccac gacgatctcg ccgctctcgg ccgcgaccac cccgaagagg gtctcgagga
     7741 cttcggtccg accggcgccc atgagcccgg cgaagccgag gatctcgccg cgacgcaggt
     7801 cgaagctcac gtccttgacg accgaaccac ggtgcaggtt gcgcaccgag aggacgacct
     7861 cgccgatctc gacgaactcc ttggggaaca tctccgtgag ctcgcgcccc accatgaggg
     7921 tgatgagctt gtcgcggtcc aactcggacg ccggcagggt cttgacgagc tggccgtcgc
     7981 ggaagaccgt gacgtagtcg gagatgttga agacctcgtc catcttgtgc gtgatgtaga
     8041 tgatcgacgc accggtggac ttgagctggc ggatcatccg gtggaggtgt tcgacctccc
     8101 gctcggtgat ggccgaggtg ggctcgtcca tgatgatgat ccgcgagtcg aaactgatcg
     8161 ccttggcgat ctcgaccatt tgggtctggg cgatggacag ggtgcgcatc cgggcccggg
     8221 gatcgatgtc gatctcccag cgctggaaca gctcacgaca cgagctgacc atctgcttgc
     8281 ggctcaccag gccgaaccgg gccacgggct cgcggccgag gaagatgttc tccgccacgg
     8341 tcatctccgg gacgggcgac agctcctggt ggatcatcga aatacccaga cccaggccgg
     8401 cggccgtgtc ggggatgtcg accttctcgc caccgaggat gatcgtggcc cccgggtcgg
     8461 cttggtacat gccgatgagg atcttcatca aggtggattt gcccgcgccg ttctcgccca
     8521 tgagggcgtg gacggttccg ggtcgaacct cgaggttgac gcccgcgagc gccctcaccc
     8581 ctgggaatgt cttggacacg tcctgcatct gcaggaggaa ctcatcgctc acggatacct
     8641 cgccctgatc gcggccgcgc ctggcgtgcc tgggactggt ggctgtgggt ttgcggtggg
     8701 cgggcgactc gtcgcccgcc caccgcgggt cactcactga ccggcaaact cactgaccgg
     8761 caactcactt accggcgaac tgtgccatgt tctcgggggt cacaaggacg tagggaacgt
     8821 cgacgtaggc cggcacggtg tccttgttgt agagcttgat cgcagcgtca acgccaccgg
     8881 cgccctggcc ctttgcgtcc tggaacaccg tcaccttgag ctcgcccgcc ttcatggcag
     8941 ccagcgcgtc acccgtcgcg tccaccccac cgacgatggt cttgtcgagc ttgcccgccg
     9001 ccttgagggc gttgatcgca ccgagcgcca tctcgtcgtt gttcgcacac acgacgtcga
     9061 tctggtcgcc ggactgcagc caggtctcca tgatcgactg gcccttctcg cgctgccagt
     9121 tgccggcctg ggtcttgatg accttcatgt ccgggttctt ggcgacgacg tccacgcagc
     9181 ccttggtccg caggacggcg gcctcgttgg acgggtcacc ctggaggatg acgacgttgc
     9241 ccttgccgtt ggccaacttg gccaactggg tcatctccag gttgccggag acgagcgagt
     9301 cggaaccgac gtaggggacg ccggccggca ggtcagcggg cttgcggttg acgtagatca
     9361 gggggatgcc ggcggcctgg gccttggccg tcatcgggcc ggccgcgtcg gtgtcgaccg
     9421 ggacgacgat gatcgcgtcg accttgttgg cgatgaaggt ctcgacctgg gccaactgga
     9481 cgtcggtctt ctcttgcgaa gcctggatgt tgacctcgac gccggcctcc gccgcgcggg
     9541 cgctgatgcc gtcggcgacc ttttggagga actggtcgag gatcgggaag gtcacgccga
     9601 tcttgagcgc cttcccgtcc ttcggcttga ccacggcgga gctgcccgcg gcagcaggag
     9661 tcgtggtggt ggcggcagcg gccttggtcg cggcggctgt cgtcgcggcg gtcgttccgg
     9721 ccgtggaaga gcaagcggcg aacaggctgg tcgtggcgag cagaagtgcc cccaccttga
     9781 gtccgtgcga gatagccatg gttcctctct ctgtgggtgg gacagggccc gcagggcctg
     9841 cggctagccc aaacgggtgc cggtgagcca cccgtcctac cggctacgtg tcgcagctgg
     9901 taagtcagac gctattgccg ccgtaacctc ctgtcaatac atacgaacaa agatgttaca
     9961 aactagtgac ccggttcggc aggcctctga ggtatgggcc gccttgacct cagagcgacg
    10021 cgataccgtt gtaccccaat gcatttcgtc cgcagtggtc ccgcgggccc tctcactgcc
    10081 agccctgtgc gcccccggag cccgtggggt ggaggacccc atcgaccctt gatctgccac
    10141 cgcgtatgtc ctgacaaccg tcacaacagt cgaggcccga ccgagacggg tctcggtcgg
    10201 gcctcaccta ctgacgcccc gcttccctgc gtcaggcgta gaagtccggc ttcgcaatca
    10261 tctgcacggg cacgaccccg tcgtcggtga gcgccttgac acccgcgtcg cagacgcagg
    10321 cggcggcata cccgtcccac gcggtcgacc ccgagtgctc gcccttcgcc gcggccgtga
    10381 tccactcctg gacctcggtg acgaaggcgc cccagaaccg ctcgttgtgg ttcatggaga
    10441 tccggttgtt gcggccgacc gagtcacgcc gggtgacgac gttctggtcg gccagcgaga
    10501 cggcgccctt ttcggcgacc agctcgcagc ggatgtcgta gccgtactgg cagttgacga
    10561 acacctcgtc gtcgacgcgc acgcctgagg ccgtctccag cacgagcacc agcgggtcct
    10621 gcaagtgctc gaagcgcagc gacgtcttct tcggcttgtc gacccgcgcc gacacgaact
    10681 cctcgccgag cagccaccgc atcgtgtcga tctcgtggat cgccgtgtcg ttgatggcca
    10741 tgtcccacgt gtagaagtcc ggcacggtgg ggttgcggtg cgcacagtgc accatgagcg
    10801 cctcaccgag gtcgccgctg tcgaggatcg ccttcatctc gcgataggag gcgtcgaagc
    10861 gccgcatgaa accgaccgtg acgagcctgc gcccgccagc ctgctcggcc tccatgatcc
    10921 gcaaagcggc gtcggccgtc ggggtgagcg gcttctcgca gaagaccggc ttccctgccg
    10981 cgatgcaggc caggacgtcc ggctcgtgcg ccggcccatg gctgcagatc atcactgcgt
    11041 cgacgtccgg gctcgcgatg agctcagccg aggtggcgta tgccgtcgcg ccgatccggt
    11101 cggcgaccgc ctgagcggtg gccgcgttga cgtcgctgac cgcgacgacc cggccgccgg
    11161 cgatgacggt gtggatgcgt tcgatgtggg cctgccccat accgccgggg ccgatcatgc
    11221 cgatccgaag agtcatcgga gggtcaccct tctgctgcta cttgaggccg aggccgaggg
    11281 acgcgaggta ctcgcgggtg gtgacggcgt tggggaacgg gtagtccttg gcgcagccat
    11341 acatgtcctg ctcacagacg acatagagct ccttgtcgag gtcggcgagc gcctcgatga
    11401 gctgacccat gtcgggcaga ccggccggcg gcgcgactga gcacccggcg tggacggcct
    11461 tgacgaacgg ccagtcctcg tcgtgggcct gggccacgag ggcctggtcc atcgccttga
    11521 tgtgaacgta ggagatccgc tcggggtact tgcggcacag ctccatcggg tcgccgccgc
    11581 catagacgat gtgcccggtg tcgaggcaga agccgacgta ctcggggtca gtggcctgga
    11641 agatccggtc gatgtcctcg ggggtctcaa tgtggctgtc accgtggggg tgcagcacca
    11701 tctgcaggcc gtagtcctcc ttcatgatcc gcccgagctt gtcggcgttc ttgatgtaga
    11761 ggttccatgc ctcggtcgac agggtgcggt cgtcggtgta ggcgccggac ttctcgtcgc
    11821 ggaacatcgg ggggaggtgg acgacgaact tcgcgccgac ggccgcgtgc gtctcgccga
    11881 tcgcgcggaa gaaggtctcg gtgtcgttcc aggcttcggc cttgtgcagg atcccccagc
    11941 cggtgccggc gacgaccgtg aagccgtgct cgtccatgac gtcacgcaac tgcgtcgggt
    12001 ccttggggaa gtagccgaac gggccggtct ccatgaccga gaagcccgcg gtctgcatct
    12061 ccttgagggc gacgtcccaa tcgatctgct tgtcgtcctg cgggaaccac acgccccact
    12121 ggtcggggca gacgccgatg gtgagcttgc tgtagcgggg gtcggtgttg cgagccttgg
    12181 actcggccat ggcagttcct ttcgcggggg ttcctctggg ttgaccctag atctgtccta
    12241 atgtgctgtc aatagaccaa ggtcatcggt ctagtggtgt gatccattcg acgcgtcggc
    12301 gagcggaagc accctgccgc agacgtgcgt gggcgcgcgg catacccctg aggtatgccg
    12361 cgcgcctcgc cgttggtgcg gctgcgtagc gcgggggcta cttgttctgc gggaagccga
    12421 ggttgaggcc gccgtggctg gggtcgagcc agcgctgggt gaccgccttg gtgcgggtgt
    12481 agaagtgcac gccctccatg ccgtgggcgt ggctgtcgcc gaagagcgat gccttccacc
    12541 cgccgaagga gtagtaggcc atcgggacgg ggatgggcac gttaatgccg atcatgccca
    12601 cctggacctc gttctggaac cgccgcgcgg cgccgccgtc gttggtgaag atcgcggtgc
    12661 cgttgccgta gaggctggag ttgatgagct ccaggccctc gtcataggac tccacgcgca
    12721 cgaccgacaa gaccggcccg aagatctcgt cggtgtagat cgacatgtcg gtgctgacct
    12781 tgtcgaacag ggttgggccg acgaagaagc cgtcagcacc cccgtggaac tcggtctcac
    12841 gcccgtcgac gaccagctcg gccccggcgc cgaccccggc ctcgatgtag ccgacgattc
    12901 ggtcgcgggc gacaccggtg acgatcggac ccatgtcgca gcccatcgag ccgtcgccgg
    12961 tcttgagggt ggccatccgc tcggagatct tcgcgatgag ttcgtcggcg atcgagtcga
    13021 cggccaagag caccgagatc gccatgcagc gctcgccggc cgagccga
//
LOCUS       DAOMZY010000050        12959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_4451_length_12959_cov_5.329355, whole genome shotgun sequence.
ACCESSION   DAOMZY010000050 DAOMZY010000000
VERSION     DAOMZY010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 12959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12959
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4451_length_12959_cov_5.329355"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1193)
                     /locus_tag="PLK64_04490"
     CDS             complement(96..1193)
                     /locus_tag="PLK64_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79032.1"
                     /translation="MSHPSNAPSNSPTDADLEDLLRVGLREAVDRADAAYLPLEVSEI
                     VAEGARTLSRRRRWQVLGAAAAAAVAVVGSWALTGGSGTRLSQQPATVPVSTVAPTTS
                     EEIFVQDERFGVRVESGEVSTALNVIGSVTPAVLQWGPPPGSAGAHEVLVLIPEADHP
                     DPGSVDVALVRDVRRLGEARQIIVNGRLVIVVRHTPPPTGMTPYAGLTYTIGGREVRH
                     LSPGIVHIDGVGAGAARNEGGDFGYAVRLEAGTVAVSLQRRVGDLDLGGGVFTLPAGS
                     PGLLRLGDGADFDMAYALVRGTPPRAVTVTGDTPSRFAGGVSWGGMTIDDTDVTLVLI
                     ALTGTDDSGSGQLRSITWTDADGGRHTIAAS"
     gene            complement(1190..1699)
                     /locus_tag="PLK64_04495"
     CDS             complement(1190..1699)
                     /locus_tag="PLK64_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HPV79033.1"
                     /translation="MTPKDRERDASFAQFVEVASPGLLRTAWLLTGSSEAAHELVQAT
                     LVKAYVRWPQIRSESAMAYCRRILANERTDRWRRTRREVPVAEVDTRSADRSDTATSD
                     DRDEVVRLLRGLPEQQRRVVVLRYYCDLSEDTVADTLGISVGTVKSSASRALAALRAQ
                     LVVQEGEIR"
     gene            1822..2937
                     /locus_tag="PLK64_04500"
     CDS             1822..2937
                     /locus_tag="PLK64_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPV79034.1"
                     /translation="MPPAPGRRPVTNPLDRESLRSRVQSALEAELDHQEQVLAELDED
                     LTPLVAPVRSLLAGGKRLRAAFAYWGYRAAGGADSDSLVRFASSMELFQAAALLHDDV
                     MDGSDTRRGMPSAHRAVAAIHAQHAWSGNADRFGEATAILAGDLCLQWTDEVYSTSGL
                     PAEHLARGRATFDRMRTQLMGGQFLDMLESVRTWEGRSTDERIAAARKVIRFKSAKYT
                     IEQPLLIGALAGGVDPAAVPGLSGYGLALGEAFQLRDDLLGVFGDPEQTGKPAGDDVR
                     EGKRTVLIAYTLDVADAGQRAELTRSFGRADVDVAGIESVRGIIRATGAVERVESDIT
                     RLTHAATHALGEPAAYLQPEASTVLGELVALATDRVT"
     gene            complement(3052..3459)
                     /locus_tag="PLK64_04505"
     CDS             complement(3052..3459)
                     /locus_tag="PLK64_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2175c family DNA-binding protein"
                     /protein_id="HPV79035.1"
                     /translation="MAEPQLASEADDDSDDPRALETLVGDWSYLPDIAERYGVALSRV
                     RRFLADRELLTHRVGPNRALAVPTDFLGPDGPRPELKGTFTVLADGGMSDVEIIRWLH
                     SVDTSLPGGSTPLAALSAGFKTEVRRRAQALAF"
     gene            3594..5600
                     /gene="pknB"
                     /locus_tag="PLK64_04510"
     CDS             3594..5600
                     /gene="pknB"
                     /locus_tag="PLK64_04510"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HPV79036.1"
                     /translation="MTSGPGLHADERVLGGRYRLLSRIADGGMATVYLAIDERLGRRV
                     AVKVLRADLARDEGFVGRFQREARLAAGLSHPNIVAVHDFHESSGVPGEELYLVMEYV
                     DGRTLREKLVDGGAMSVRQATHIITAVLAALDEAHRADLVHRDVKPENVLVRDDGTIK
                     VTDFGLARAVTSSTRTSTTGILMGTVSYLAPEQVDGDRADARSDVYAAGLMLYELLTG
                     QRAFQGENPAHVMYQHVHGEVPLASDRVSTVPLELDQVIARATARSPDDRPDTAGDLI
                     QVLRDAIRQLSDDEFDAAPVFLAGMAPGTQPVARTASPTKTLDADLVGAEGDPSPTER
                     FAAVELGATQRLRTIELDEGGDGTGEVSAPAGEPVKQKRRRRIWPWLLTLLLLVGGGV
                     GGWWWFDGTGPGSLRIVPTLVHLSLADAEKALARVDLRSATTEAFSETEPKGQVISAD
                     PKPGAEVSKRSSVALVVSKGQERYAVPTLTAVARGDVERLLKERSLALGTVTEDFSET
                     VAKDVVISQDPPPDTLAKRDTPVNVVISKGRAPIPVPNVVNKTADEAQKALEALGLKV
                     VRGDPVNSETVASGAVVSQTPATGTLFKGDAVTIIPSKGPVLVQVPDVTLKKVNDATK
                     ILKDLGLEVKVSQIFGGVLKTVRFQDPAAGTMVRRGTVITLTVI"
     gene            5617..6525
                     /locus_tag="PLK64_04515"
     CDS             5617..6525
                     /locus_tag="PLK64_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPV79037.1"
                     /translation="MAMSPARARTVATILLVTLTAVWGSTFFLIRDLVQTVPPADFLA
                     VRFTLAAVLMVVIFWRQLRGLSRRDWLVSAALGSLYGFAQILQTIGLAHTDASVSGFV
                     TGTYVVLTPVFTALLLRERVPASTWYAVLLATAGLALLSLNGFSVGLGEGLTLGAAAL
                     YALHIVGLGRLSTPDRATGMSAVQMIAIAVICVVAAVPDGMVWPSGVGQWGSVVYMAT
                     AAGVLALWAQTWGQAHMSATRAAIVMTLEPVFAAGFAVAFGGEVLTWRMLVGGALVLT
                     AMYTVELIGSRPDAPNRPAEALHHEV"
     assembly_gap    6737..6836
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6961..8016)
                     /locus_tag="PLK64_04520"
     CDS             complement(6961..8016)
                     /locus_tag="PLK64_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HPV79038.1"
                     /translation="MTLWRADLLSDTVTRPTPAMRQAMARAEVGDDVFGEDPTVQALQ
                     ERVAGLLGHEAALFTPSGSMANQLGLRLHVRPGEELLCDSLAHVVRAELGAAAAFSGI
                     TSRTWTAPRGLLDPPDAIALLQTGVGPYQVCTSLVVVENTHNFGGGTVQPLWAIRQVR
                     AATRPRGVAMHLDGARLWNAHVASGVALAEYAAHFDTVSVCLSKGLGAPVGSLLVGSA
                     DAIAEARIWRKRYGGGMRQVGILAAAGLYALDHHVERLADDHARAQRAARAFADAAPG
                     CVDPETVETNIVVLATTDTGWSAAELVAAAAAQGVRGYAAGPEALRLAWHLDVDDAAT
                     DYAIDVIATLLRARPAP"
     gene            complement(8060..9400)
                     /locus_tag="PLK64_04525"
     CDS             complement(8060..9400)
                     /locus_tag="PLK64_04525"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007271816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HPV79039.1"
                     /translation="MHGQLAAAAAERTAGQQPTWPDQGALAVAVAELAQYPPLVFAGE
                     CDVMRERMAATARGEAFVLMGGDCAETFAGATANNIRDRIKTVLQMAAVLTYGASVPV
                     VKVGRMAGQYAKPRSADTETRAGQTMPAYRGDMVNDFAFDPVARIPDPQRLVTAYHRA
                     SATLNLVRAFTTGGFADLRRVHDWNTGFVENAANARYERLAHDIGKAMKFMSACGADF
                     DAMRTTEFFAAHEALLLDYERPLTRIDSRTGQAYDTSAHFIWLGERTRELEGAHVDFL
                     ARVRNPIGVKLSAKADPDDVLRLIDRLDPQREPGRLSLITRMGASRVTEALPALIERV
                     HGSGATVTWICDPMHGNTFESQGGYKTRNFDDIVEEVRGFFAVHKELGTVPGGLHVEL
                     TGNDVTECIGGSTKILDADLEKRYETVCDPRLNHRQSLELAFLAAEMLSGDVPS"
     gene            9561..10388
                     /locus_tag="PLK64_04530"
     CDS             9561..10388
                     /locus_tag="PLK64_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HPV79040.1"
                     /translation="MTFSIVAGGGTAYGVAVASKFLAVGSVVPAAVPGIGAVATQSFA
                     KVSYKADSLALLRAGASASDVVARITDADPLRETRQVGVVGDGGAATFTGADCSDWAG
                     GATGGNDTDGWYAVQGNILVGPQVVQAMEQAWLASTNLPLSRRLLAALLAGDAAGGDR
                     RGRQSAALYVVDESAGYDGSGIVVDLRVDEHDDAASELARLVELNDLYFGSPEDVQPL
                     QGPLADEVRERLGKLGYRGELDAALGEWAGVENYEMRLTPDGIDARVLAQLRAATGG"
     gene            10432..10929
                     /gene="tpx"
                     /locus_tag="PLK64_04535"
     CDS             10432..10929
                     /gene="tpx"
                     /locus_tag="PLK64_04535"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013348274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HPV79041.1"
                     /translation="MATTALRGTPVSTSGELPDVGDHAPEFTLTSTSLADVSSEGLVG
                     SRVVLNIFPSVDTGVCAASVRRFNELAGSLDNTTVVNVSLDLPFALSRFCGAEGIENV
                     TSASAFRSTFGDDYGVRMVDGGLRGLFARSIVVLDTDGTVLHTQVVPEIAQEPDYDAA
                     INALS"
     gene            complement(11012..11764)
                     /locus_tag="PLK64_04540"
     CDS             complement(11012..11764)
                     /locus_tag="PLK64_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPV79042.1"
                     /translation="MKGQANNPRQPGPDGVDPLAGADGDLGSGLADRPLRVVVADDHP
                     MWRDAVARDLVDAGFSVVGTAADGPSAVSRTLATAPDVLVLDLNMPGMRGSAVCAELA
                     AAGSPARILVLSVSGEPEEVLSTIKAGATGYLVKSASREEFLEAVRRTAEGRAVFTAG
                     LAGLVLGEFRRLGQQPPPAEGDVPAAPELTERETEVLRLVATGLSYKQIAAQLFISHR
                     TVQNHVQNTLAKLQMHNRVELVRYAIERGLHE"
     gene            complement(11764..12915)
                     /locus_tag="PLK64_04545"
     CDS             complement(11764..12915)
                     /locus_tag="PLK64_04545"
                     /inference="COORDINATES: protein motif:HMM:NF039922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5931 domain-containing protein"
                     /protein_id="HPV79043.1"
                     /translation="MTPQSTPATDDDSLVLSALWGALRVFRVLALVYALWSIWVRHAE
                     MARLDLALMVIGVLSVWTAYMYVSRRRDLRIHVMEMVLASAAVMSTRWFDTPLSATTG
                     DTTIPGVWQNVPVVAIALILGWRGGLIASLVITAVMIAQVGQFDSEPLSNAGLIVMLG
                     TCLGFGADVARTEQAELRRALARQAEVAERDRLARVVHDGVLQALAFIHRRGRDIGGE
                     SARLGMIAAEQELRLRALVIGMPLPELEVAIAGPVDLRRALDAAVTESATLVASAEPV
                     ELDSAKAQEVVAAVEAALDNVRKHAGPQAQAWVVVDDLGADVVVTVRDDGPGVSQDRI
                     AEAAQSGRLGVSSSIKGRIEDLGGRARYLTSPGGGTMVEMWIPKGRAAT"
BASE COUNT         1971 a         4499 c         4425 g         1964 t
ORIGIN      
        1 cctacgagcc ggtatgccgc ccgcctcggc gccgtccacg tcccggtacg tagccgaagc
       61 ccccaaccac cagcgcccct ccagacgcca cggggtcagg acgcggcgat ggtatgccgc
      121 ccgccatccg cgtcggtcca ggtaatcgag cgcagctgcc cggaaccgga atcgtccgta
      181 ccggtaagag cgatgaggac gagcgtcaca tcggtgtcgt cgatcgtcat acctccccag
      241 gagactccac ccgcgaaccg ggacggcgtg tccccggtga cggtcaccgc tcgcggaggt
      301 gtccctcgga caagggcgta agccatgtcg aagtcagccc cgtcgccgag gcggagtaac
      361 cccggggacc ccgcgggcag ggtgaagacg cccccgccga ggtcgaggtc gcccacgcga
      421 cgctggaggc tcacggcgac cgtgcccgcc tccaggcgca cggcataccc aaaatccccg
      481 ccctcgtttc gggcggcacc cgcccccaca ccgtcgatgt gcacgatccc cgggctgaga
      541 tgacggacct cccgcccgcc gatggtgtag gtgagaccgg cgtacggggt catccccgtg
      601 ggcggcgggg tgtggcgcac cacgatgacc aggcgcccgt tcacgatgat ctgcctggcc
      661 tcacccagcc gtcggacatc ccggacgagc gcgacgtcca cagagccggg gtccgggtga
      721 tccgcttcgg ggatcagcac gaggacctcg tgagccccag cgctccccgg cggcggaccc
      781 cactgcagca cggcgggagt gaccgagccg atgacgttca acgctgtgga gacctcaccg
      841 ctttccaccc ggacaccgaa ccgctcgtcc tgaacgaaga tctcctcgga cgtcgtcggg
      901 gcaacggtgg acaccggcac cgtggccggc tgctgcgaca accgcgtgcc agagcctccg
      961 gtcagagccc agctgccaac gaccgcgacc gccgcagcag ccgcggctcc caacacctgc
     1021 caacgccgcc gccgactcag cgtgcgggcc ccctcggcga cgatctcgct cacctccagg
     1081 ggcaggtatg ccgcgtccgc ccgatccacg gcctcacgca gccctacccg caggaggtct
     1141 tcgaggtcgg cgtccgtggg tgagttggag ggcgcattgc tggggtggct catcggatct
     1201 ctccttcctg gacgaccaac tgggctcgca gcgctgccaa agctcgggat gcgctggact
     1261 tcaccgtgcc gacggagatt cccagggtgt cggcgacggt gtcttcggag aggtcgcagt
     1321 agtaacgcag gaccacgacc cggcgctgtt gctcggggag cccgcgcagc agccgcacga
     1381 cctcgtcccg atcgtccgag gtcgcggtgt cgctgcggtc ggccgatcgg gtgtccactt
     1441 ccgcgaccgg cacctcgcgg cgcgttcgcc gccaccgatc ggttcgttcg ttggccagga
     1501 tccgccggca gtaggccatg gccgactcac tgcggatctg cggccaccgg acgtaggcct
     1561 tgaccagcgt ggcctggacc aactcgtggg ccgcttcgga cgaaccggtc agcaaccagg
     1621 cggttcgcag tagcccgggc gaggcgacct cgacgaactg cgcgaacgag gcgtcccgct
     1681 ccctgtcctt cggtgtcacg gctccccctt gttgttgcct tcaccctcct ctacggagca
     1741 caagcccaga aggttgcctc ggtcctgacc cggtgtgagg gcaccgtagg ctggggcgac
     1801 ctgttcgcgc gcggcatacg aatgccgcct gctcctggaa ggcgcccggt gaccaacccc
     1861 ctggaccgcg agtcgctgcg ctcgcgggtg cagtcggctc tcgaggcgga gctggaccac
     1921 caggagcagg tcctcgccga gctcgacgaa gacctcaccc ccctggtcgc accggtccgc
     1981 tcgctgctgg cgggaggcaa acgactgcga gccgccttcg cctattgggg ctaccgcgcc
     2041 gcgggtggcg ccgactccga ctcgctcgtg cggttcgcca gctcgatgga gttgttccag
     2101 gcggccgcgc tgctgcacga cgacgtcatg gacggcagcg acacccgcag aggtatgccg
     2161 tccgctcacc gcgccgtcgc ggccatccac gcccagcacg cctggtcggg caatgccgat
     2221 cgcttcggtg aggcgaccgc catcctcgcc ggcgatctgt gtctgcagtg gaccgacgag
     2281 gtctactcca cgagcgggct gcctgccgag caccttgcca ggggccgagc gaccttcgac
     2341 cggatgcgca cccagctcat gggtggccag ttcctcgaca tgctcgaatc ggtgcggact
     2401 tgggagggtc gctcaacgga tgagcgaatt gccgctgccc gcaaggtgat ccgattcaag
     2461 agtgcgaaat acaccatcga gcaaccgttg ctcatcggtg ctctggccgg aggtgtggat
     2521 ccggcggccg tgcctgggct ctccggttat gggctggcgc tcggtgaggc gttccagttg
     2581 cgcgacgacc tgcttggcgt gttcggcgac cccgagcaga ccggcaagcc ggccggtgac
     2641 gacgtgcgcg agggcaagcg gaccgtgctg atcgcctaca cgctggacgt ggccgatgcg
     2701 gggcagcgag ccgagttgac ccgatccttc gggcgggccg acgtggacgt cgccgggatc
     2761 gagagcgtgc gaggcatcat tcgggcgacg ggcgccgtcg agcgcgtgga gtcggatatc
     2821 acccggctca cccacgccgc gacccacgcg ctgggtgagc ccgcggcata cctgcagccc
     2881 gaggccagca ccgtcctggg cgagttggtc gcgttggcga ccgaccgggt cacctgacct
     2941 gcacgggcac tattggcacg gcgcggcgcg gcgcactcat ggcgtgcgtt gccgatgctg
     3001 acgcaatctc accaagattg cgtcgggaca cgcagcggtg cctcgcatcg atcagaaggc
     3061 caacgcctga gcgcgtcgcc tgacctcggt cttgaaaccg gctgagaggg ctgccagtgg
     3121 ggtgctgccg ccaggcaggc tggtgtcgac gctgtgcaac caccggatga tctcgacgtc
     3181 gctcatgcca ccatcggcca ggaccgtgaa ggtccccttg agctcgggtc gtggcccatc
     3241 ggggccgagg aaatccgtcg ggacggcgag ggcgcggttg ggtcccaccc ggtgggtgag
     3301 caactcgcgg tcggccagga accggcggac gcgggacagc gccacgccat agcgctccgc
     3361 gatgtcgggt aggtaggacc aatcaccgac gagggtctcc aaggcgcgcg ggtcgtcgga
     3421 atcgtcgtcg gcttcggacg cgagttgagg ttcggccacg cccccagcct gccagaagcg
     3481 gccgcgctcc gaccaccgtc acggcgcacg atcgcctcgg gagtcacatc cggcactccc
     3541 gtcacagtgc gcgcctgccg gggcgcacgg cataccccac gtaaactcca cccgtgacgt
     3601 ctgggccggg cctgcacgcc gacgagcgtg tgctcggcgg gcgctaccgg ctgctctcgc
     3661 ggatcgccga cgggggtatg gccaccgtct acctggccat cgacgaacgg ctcgggcggc
     3721 gggtcgcggt caaggtgctg cgcgccgacc tggcacgcga tgaggggttc gtcggacgct
     3781 tccaacgcga ggctcggctg gccgcgggcc tgtcccatcc caacatcgtc gcggtccacg
     3841 acttccacga gtcgtccggg gtccctggcg aggagctcta cctcgtcatg gagtacgtcg
     3901 acggccggac cctgcgggaa aagctcgtcg atggcggtgc catgtcggtc cggcaggcca
     3961 cacacatcat cacggccgtt ctcgccgccc tcgatgaagc gcaccgcgcc gacctggtcc
     4021 atcgcgacgt caagcccgag aacgtcctgg tgcgcgacga cggcaccatc aaggtcaccg
     4081 acttcgggct agcgcgcgcg gtcacgtcca gcacgcgcac cagcacgacc ggcatcctca
     4141 tgggcaccgt ctcctacctc gcgcccgaac aggtcgatgg agatcgcgcc gacgcccgca
     4201 gcgatgtgta tgccgcgggc ctcatgctct acgaactgct caccgggcag cgggccttcc
     4261 agggcgagaa ccccgcccat gtgatgtacc aacacgtcca cggcgaggtg ccgctggcct
     4321 ccgaccgggt gagcaccgtg ccgttggaac tcgaccaggt catcgccagg gcgaccgcac
     4381 gctccccgga cgaccggccc gacaccgccg gagacctcat ccaggtgctc cgcgacgcga
     4441 tccgacaact gtccgacgac gagttcgacg cggcgccggt cttcctcgcc gggatggcgc
     4501 cggggaccca accggtcgct cgcaccgcca gcccgacaaa gacactggac gctgacctcg
     4561 ttggcgcaga aggcgatccg agccccaccg agcgtttcgc ggcggtcgaa ctcggcgcca
     4621 cccaacgact gaggacgatc gagctcgacg agggcggcga cggcacgggc gaggtgtcag
     4681 caccggccgg cgagccggtc aagcagaagc gacggcggcg gatctggccc tggctgttga
     4741 ccctcctgct gctcgtcggc ggcggtgtcg gtggctggtg gtggttcgac ggcaccggcc
     4801 ccggctcgct gcggatcgtg ccgaccctgg tccatctctc gctcgccgat gccgagaagg
     4861 cgctcgcgag ggtcgacctg cgctcagcaa cgaccgaggc cttcagcgaa accgagccca
     4921 agggccaggt catctccgcc gacccgaagc ccggagccga ggtgtccaag cgctccagcg
     4981 tcgccctcgt ggtctccaag ggccaggagc ggtatgccgt ccccaccctc accgccgttg
     5041 ctcgcggcga cgtcgagcgg ctgctcaagg aacgctccct cgccctcggg acggtcaccg
     5101 aagacttcag cgagaccgtg gccaaggacg tggtcatcag tcaggacccg cctcccgaca
     5161 cactggccaa gcgagacacc cccgtcaacg tcgtcatcag caagggtcga gcgccgatcc
     5221 ccgtgcccaa cgtcgtcaac aagacggctg acgaggcaca gaaggcactc gaggcgctcg
     5281 gcctcaaggt ggtccgaggc gacccggtca acagcgagac ggtcgcgtcc ggggccgtcg
     5341 tctcgcagac cccggccacg ggcacgctct tcaagggcga cgcggtgacg atcatcccgt
     5401 ccaagggccc ggtgctcgtc caggtgcccg acgtcaccct caagaaggtc aatgacgcca
     5461 ccaagatcct caaggatctc ggcctggagg tgaaggtctc ccaaatcttc ggcggggtgc
     5521 tcaagactgt tcgcttccag gacccggccg ccggcaccat ggtgcgacgc ggcacggtca
     5581 tcacgttgac cgttatctga cagcgcggca cacctcatgg ccatgtcccc tgcccgcgcc
     5641 cggaccgtcg cgacgatcct gctggtgacg ctcaccgcgg tgtggggatc gacgttcttc
     5701 ctcatccgcg acctcgtgca gacggtgccg ccggccgact tcctcgcggt gcgattcacc
     5761 ctcgccgcgg tgctcatggt cgtcatcttc tggcgccaac tgcgcgggct ttctcgacgc
     5821 gactggctgg tctcggccgc actggggtcg ctgtatggct tcgcccagat cctccaaacg
     5881 atcgggctgg cccacaccga cgcctcggtg tcggggttcg tcaccggcac ctatgtcgtc
     5941 ctcacgcccg tcttcaccgc gctgctcctg cgtgagcgcg tccccgccag cacttggtat
     6001 gccgtgctcc tcgccaccgc gggcctggcc ctgttgtctc tcaacgggtt ctcggtcggt
     6061 ctcggggaag ggctgactct tggggctgcg gcgctctacg ccctgcacat cgtcgggctc
     6121 ggtcgcctgt cgacccccga ccgagccacc gggatgtcgg ccgtgcagat gatcgccatt
     6181 gcggtgatct gtgtcgtggc ggcggtcccc gacggcatgg tgtggccgag cggcgtcggt
     6241 caatgggggt cggtcgtcta tatggcgacg gccgctgggg tgctggcgct gtgggctcag
     6301 acgtgggggc aggcgcacat gtcggccacc cgggcggcga tcgtcatgac cctggagccg
     6361 gtgtttgcgg ccgggttcgc cgtcgcgttc ggcggcgagg tactcacgtg gcggatgctc
     6421 gtcggtggcg cgctcgtgct gaccgcgatg tacaccgtcg agctcatcgg gagccgccct
     6481 gatgccccca accgcccggc cgaagccctg caccacgagg tgtgatgacg cccgccgagg
     6541 atggttcgat cacaccaaac tttctgcgag cccgcccggg cccacacacc gtcacgcgag
     6601 acgacgacga ggacgcctgg cttcaccacc tcatcacgaa gctctcgtca cacggcgacg
     6661 aaacggcgac cgtctgctgc tcgcgaaacc cccggcgagt gtgagggatt cgggcgcatc
     6721 ctggtgtgct ggtcgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgcg
     6841 aaacccccgg cgagtgtgag ggattcgggc gcatcctggt gtgctggtcg cgaaaccccc
     6901 ggcgagtgtg agggattcgg gccggggacg aggctgcggc ccattgctgg tgacgctcgg
     6961 tcagggcgcc ggacgagccc tcagcagagt ggcgatgaca tcgatcgcgt agtccgtagc
     7021 cgcatcgtcg acgtcgaggt gccacgcgag gcgcaacgcc tctggccccg cggcataccc
     7081 gcggacaccc tgcgccgcgg cggcggcgac gagctctgct gctgaccatc cggtgtcggt
     7141 cgtggcgagc acgacgatgt tggtctcgac cgtctcggga tcgacacacc ccggcgcggc
     7201 atcggcgaag gcgcgtgccg ctcgctgggc gcgggcgtga tcgtcggcca accgctcgac
     7261 gtggtggtcg agggcataga ggccggccgc ggccaagatg ccgacctggc gcatcccgcc
     7321 gccatagcgc ttgcgccaga tgcgggcctc ggcgatcgcg tcggccgagc cgacgaggag
     7381 cgaccccacc ggcgcaccca accccttgga gaggcagacc gagacggtgt cgaagtgcgc
     7441 ggcatactcc gcgagcgcga cacccgacgc gacgtgcgcg ttccacagtc gcgccccgtc
     7501 caggtgcatg gcgacgccgc gcgggcgggt agccgctcgg acctgacgga tcgcccacag
     7561 cggttggaca gtgcccccgc cgaagttgtg ggtgttctcg acgacgacga gtgacgtgca
     7621 cacctggtag ggcccaaccc cggtctgcag caacgcgatc gcgtcaggcg ggtcgagcag
     7681 acctcgaggt gcggtccagg tgcgggaggt gatgcccgag aacgccgccg cggcgccgag
     7741 ctcggcccgg acgacgtggg cgaggctgtc gcagagcagt tcctcgccgg gccgcacgtg
     7801 cagacgcagg cccaactggt tggccatcga gccggacggt gtgaacagcg cggcttcgtg
     7861 gcccagcaac ccggcgacgc gctcctgcag ggcctggacg gtcgggtctt cgccgaagac
     7921 gtcgtcaccg acctccgcgc gagccatcgc ctggcgcatc gccggcgtgg gccgagtgac
     7981 tgtgtcggac aacaggtcag cgcgccacag ggtcacagga ctgatctcct tcagatcatc
     8041 gacgggtatg ctgcgcggct caggacggca cgtcccccga cagcatctcg gcggcgagga
     8101 aggccaactc caggctctgg cggtggttga ggcgcgggtc gcagaccgtc tcgtaacgct
     8161 tctccaggtc ggcgtccagg atcttcgtgg agccgccgat gcactcggtg acgtcgttgc
     8221 cggtgagttc gacgtgcaac ccgcccggca ccgtgcccaa ctccttgtgg accgcgaaga
     8281 agccgcgcac ctcttcgacg atgtcgtcga agttgcgggt cttgtagccg ccctgggatt
     8341 cgaaggtgtt gccgtgcatc gggtcgcaga tccaggtgac ggttgcgccg gacccgtgga
     8401 cgcgctcgat gagggcgggc agggcctcgg tgacgcggct cgcgcccatc cgggtgatga
     8461 ggctcagccg gcccggctcg cgctgcgggt cgagccggtc gatgagccgc agcacgtcgt
     8521 ccgggtcggc cttggccgac agcttgacgc cgatggggtt gcggacccgg gcgaggaagt
     8581 cgacgtgggc accctcgagc tcccgggtgc gctcgccgag ccagatgaag tgggccgacg
     8641 tgtcgtaggc ctgcccagtg cgcgagtcga tccgcgtcag cggacgctcg tagtccagca
     8701 gcagcgcttc atgggcggca aagaactcgg tggtgcgcat cgcgtcgaag tcggcgccac
     8761 aggcggacat gaacttcatc gccttgccga tgtcgtgggc cagccgctcg taacgggcgt
     8821 tggcggcgtt ctcgacaaag cccgtgttcc agtcgtggac gcgcctcaga tcggcgaaac
     8881 cgcctgtggt gaaagcccgg acgaggttga gggtcgccga ggcgcggtgg tatgccgtga
     8941 ccaaccgttg cgggtcgggg atccgggcca ccgggtcgaa cgcgaagtcg ttgaccatgt
     9001 cgccgcggta ggccggcatg gtctgcccgg cccgggtctc ggtgtcggcg ctgcgcggct
     9061 tggcgtactg accggccatc cggccgacct tgaccaccgg caccgaggcg ccgtaggtga
     9121 gcacggcggc catctgcagc accgtcttga tccggtcgcg gatgttgttg gccgtcgccc
     9181 ccgcgaacgt ctcagcacag tcgccgccca tgaggacgaa cgcctcgccg cgggcggtcg
     9241 ccgccatccg ctcgcgcatg acgtcgcact cccccgcaaa caccaggggc gggtattgcg
     9301 cgagttcggc gaccgcgacc gccaaagccc cctggtcagg ccaggtgggt tgttgtccgg
     9361 cggtgcgttc tgccgcggct gccgcgagct gcccgtgcaa gtcgtcgttg gggttcgtgc
     9421 tcactgggac agcgtagttg cgtcccagtc cgtgggatgc gcacggtccg gatatcgatc
     9481 atccagtgcc tgacgacagg ccgggttttc cgctcaggca cttcgctcgc agggcgttcc
     9541 tcaggtctag gtttggcgtc atgacattct cgatcgtggc cggcgggggc acggcatacg
     9601 gagtggcggt ggcgagcaag ttcctcgcgg tggggtccgt agttcccgcc gcggtccccg
     9661 gaatcggcgc cgtggcgacc cagtccttcg ccaaggtgtc ctacaaggcc gattcgctgg
     9721 cgctcttgcg cgctggagcg agcgcgagcg acgtcgtcgc ccggatcacc gacgccgacc
     9781 ccctgcgcga gacccgtcag gtcggcgtcg tcggggacgg cggcgcagcg acgttcaccg
     9841 gcgccgactg ctcggactgg gccggcggag cgaccggcgg gaacgacacc gacggctggt
     9901 atgccgtgca aggcaacatc ctcgtcggcc cgcaggtcgt ccaggcgatg gagcaggcct
     9961 ggctcgcaag caccaacctg ccgttgtcgc gtcgcctcct ggccgccctc ctcgccggtg
    10021 acgcggcggg tggcgatcga cgtgggcgcc agagcgccgc gctctatgtg gtggacgaat
    10081 cggcgggata cgacggcagt gggatcgtgg tggacctgcg ggtcgacgag cacgacgacg
    10141 cggcttcgga gttggccagg ctcgtcgagc tcaacgacct gtatttcggg tcccccgagg
    10201 atgtccaacc cctgcaggga ccgttggcgg acgaggtgcg cgagcggctg gggaagttgg
    10261 ggtatcgcgg cgaattggac gccgccctcg gggaatgggc cggggtcgag aactacgaga
    10321 tgcgcctgac ccctgacggc atcgacgcgc gcgtgctcgc ccagttgcgg gcagcgaccg
    10381 ggggctgagg accaggcgcg ccgaacggca tacccgtgat aggaacgtgt catggcaacc
    10441 acggccctgc gcggcacccc cgtctccact tccggcgaac tgcccgatgt cggcgaccac
    10501 gctcccgagt tcacgctgac cagcacctcg ctggccgacg tctcctccga gggcctggtg
    10561 ggctcgcggg tggttctcaa catcttcccg agcgtcgaca cgggggtgtg cgccgcgagc
    10621 gtgcgccgct tcaacgagct cgctggatca ctggacaaca cgacggtggt caacgtgtca
    10681 ctcgacctac cgttcgctct gagccggttc tgcggcgctg agggcatcga gaacgtcacc
    10741 tccgcatcgg ccttccgcag cacgttcggc gacgactacg gcgtgcggat ggtcgacggc
    10801 ggcctgcgtg ggctgttcgc ccgttcgatc gtcgttctcg acaccgacgg gacggtcctg
    10861 cacacgcagg tcgttcccga gatcgcccaa gagcccgact acgacgcagc gatcaacgcg
    10921 ctgtcctgat ctggtgagcg gtcgggccgc atcggtccga ccgcctgctc gaccgaccgc
    10981 ccgcgtgtgt cggcgtgcgc gtcggcagcg atcactcgtg caggccgcgc tcgatcgcgt
    11041 agcggaccag ttcgacccgg ttgtgcatct gcagcttggc cagggtgttc tggacgtggt
    11101 tctgcaccgt gcgatgcgag atgaacaact gagctgcgat ctgcttgtag gacaacccgg
    11161 tcgccaccag gcgcagcacc tcggtctcgc gttcggtcaa ctcgggagcc gctggcacgt
    11221 cgccctcggc gggcggaggt tgctgtccga ggcgacggaa ctcgccaagc accagcccgg
    11281 ccagccccgc cgtgaagacg gcgcgacctt cggcggtgcg gcgcacggct tcgaggaact
    11341 cctcccggct ggctgatttg acgagataac cggtcgcgcc cgccttgatc gtcgagagca
    11401 cttcctcggg ctcgccgctc accgacagca cgaggatccg ggcgggcgac ccagcggcgg
    11461 ctaactcggc gcagacggcc gaccctcgca tgccgggcat gttgaggtcc aggaccagca
    11521 catctggtgc cgtcgccagg gtccgagaga cggctgacgg tccgtcggcc gccgtgccca
    11581 ccacgctgaa gccggcatcg acgaggtcac gcgccaccgc atcacgccac atcgggtgat
    11641 cgtcggcgac gaccacgcgc aacggccgat cggcgaggcc gctgcccaga tcgccgtcgg
    11701 cgccggcgag cgggtcgacc ccgtccgggc ccggctgcct cgggttgttc gcttgtccct
    11761 tcatcaggtg gccgccctcc ccttgggaat ccacatctcg accatggtgc cgccgcccgg
    11821 gctggtcagg taacgggctc gtccgccgag gtcctcgatc cgccccttga ttgaggacga
    11881 cacgcccagt cgcccgctct gggccgcctc ggcgatccgg tcctgggaga cccccgggcc
    11941 gtcgtcgcgc accgtgacga cgacgtcagc tccgagatcg tcgacgacca cccacgcctg
    12001 tgcttggggg ccggcatgtt tgcggacgtt gtccaaggcg gcctcgacgg cggcgacgac
    12061 ttcctgggcc ttggcggagt ccagctcgac cggctcggcg ctggcgacga gcgtggcgga
    12121 ctcggtgacc gcggcatcca gggcgcggcg caggtcgacc ggccccgcga tggcgacctc
    12181 caactcgggc aacggcatcc cgatgaccag cgcccgcagc cgaagttcct gctcggcagc
    12241 gatcatcccc aagcgggcgc tctcgccacc gatgtcccgg ccacgacgat ggatgaacgc
    12301 caacgcctgc aacaccccat cgtggacgac gcgggccaac cgatcccgtt cggcgacctc
    12361 ggcctgtcgg gccagtgcgc gacgcagttc ggcctgctcg gtgcgcgcca cgtcggcgcc
    12421 gaaacccagg caggtcccga gcatgacgat gaggccggcg ttcgacaacg gttcggaatc
    12481 gaactgcccc acctgcgcga tcatcacggc cgtgatgacg agggaggcga tcaacccgcc
    12541 ccgccagccg aggatgaggg cgatcgccac gacggggacg ttctgccaga cgcccgggat
    12601 cgtcgtgtca ccggtcgtgg ccgacagcgg ggtgtcgaac caccgcgtcg acatcacggc
    12661 ggccgaggcg agcaccattt ccatcacgtg gatgcgcagg tcgcggcggc gcgagacata
    12721 catgtatgcc gtccacaccg aaagcacgcc gatcaccatg agcgcgaggt ccagtcgagc
    12781 catctcggca tgccggaccc agatgctcca cagggcatac acgagggcaa gcacccggaa
    12841 gacgcgcaag gctccccaga gggcgctgag gacgagcgag tcgtcgtccg tcgccggtgt
    12901 gctctggggg gtcatggtgc gaaactaccg cgcgacaggc tcagctggcc cgaaggcga
//
LOCUS       DAOMZY010000051        12914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_4472_length_12914_cov_5.738782, whole genome shotgun sequence.
ACCESSION   DAOMZY010000051 DAOMZY010000000
VERSION     DAOMZY010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 12914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12914
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4472_length_12914_cov_5.738782"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..489
                     /locus_tag="PLK64_04550"
     CDS             79..489
                     /locus_tag="PLK64_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HPV79044.1"
                     /translation="MAAVAATRPGERGSAVAEFVMVSTLVLVLGMGVFQLGLVLHVRN
                     TLIACAAEGARLGARADVPTSDASERTGALVTDTLSAAYAQDISTRRTVTDAGVRVVE
                     VTVVAPLPVIGLIGPGGSMTVVGRAFDERQVIEP"
     gene            486..956
                     /locus_tag="PLK64_04555"
     CDS             486..956
                     /locus_tag="PLK64_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HPV79045.1"
                     /translation="MTRLTGLVRWVRLRVGRPDQGRAIVEFVFLGVLLLVPLIYLVMT
                     LAVLQAAAYSATTAAREAGRAFTTAPAESQGMPRARAAAQVAFEDFGFLPEETSVAIT
                     CDGSPCLRPDARVSVTTSIRVRLPYLPAFLGDAVPAAIPVSATHVATVGRFGGS"
     gene            953..1462
                     /locus_tag="PLK64_04560"
     CDS             953..1462
                     /locus_tag="PLK64_04560"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HPV79046.1"
                     /translation="MSGPRRVSAAVRRFRAAVRRRRAESGQVSVLILGMVMLAMLLIV
                     GGVDVTAAQLARVRLVDAADAAALDAADALDEATAYRSGLSDAVVLSSATVKEAAAAY
                     LSVRPKPEGVRAWGIAPGTGAQGGDTAVVVLDAIVELPMTGGLLAALGKEIAVHVEAK
                     ARAPLLPVP"
     gene            complement(1584..>2204)
                     /locus_tag="PLK64_04565"
     CDS             complement(1584..>2204)
                     /locus_tag="PLK64_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79047.1"
                     /translation="VASRFMGRIASEVIQANQAVASKVPGLGPLVSFGTSTASRLRGA
                     ADRQFEGLIGDAVGKGGTFAVRRLNRIFVETLRDPTTLEAILQVWDQTSGERVIGLGA
                     YAAHAEVSGVVDAAHELAVTALGTEHAARVATLVVDALFEWFGGYTLAELLDQLEWPR
                     DEVIAHLVPLASFVVDTLRASGDLERLLRAQLEPFYASAEVADLLR"
     assembly_gap    2205..2304
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2712..3824
                     /gene="prfB"
                     /locus_tag="PLK64_04570"
     CDS             2712..3824
                     /gene="prfB"
                     /locus_tag="PLK64_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPV79048.1"
                     /translation="MAVDFGEEIKHLRATMDSVREVTNLDALTTEIARLEAEASVPNL
                     WDDPDRAQQVTSALSRANSERERVTGMDSRVEDLEVLVEMGTEEGDADTLAEAERDLA
                     KLKKDVGELEVRTLLNGEWDAREAVVTIRSGAGGVDAADFAEMLMRMYLRWAERHNYP
                     TTVLDTSYAEEAGLKSATFEVKVPYAFGNLSVEAGTHRLVRISPFDNQGRRQTSFAAV
                     EVVPLIEQTDHIDIPENELKIDVFRSSGPGGQSVNTTDSAVRMTHIPTGIVVSMQNEK
                     SQIQNRAAALRVLQSRLLLQKKAEEAATRKEMAGDVKASWGDQMRSYVLQPYQMVKDL
                     RTDHEVGNPSAVFDGDIDDFIEAGIRWKRNLEKGNG"
     gene            complement(3876..5129)
                     /locus_tag="PLK64_04575"
     CDS             complement(3876..5129)
                     /locus_tag="PLK64_04575"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV79049.1"
                     /translation="MAAAGEGEALTRRGIPLDPQLRVLAIGAFATRFGSGAVMTTSAI
                     YFTRHLGFTVGEVALAVSVAAAAAMIAALPAGHLGDTRGPREVLNVIMLALAVATPLP
                     VLATSPWVLTLLLAVEAALASAANAVRNGLTAQVAIGGRGVQFKAYLRAVTNSGIALG
                     SVVGGAALVVDTRAAYVSVFVMAGVLCGFAAWNTTRLTHLPPYRRPDGEPRLGVLRDL
                     PFVAVVLSTSMFSVHFVAAELGLALFVSQRTQAPQVMVAVLLLVNTVAVASLQVRLST
                     SADSVATGGRALVRGGALIAVGFALIGLADGSSAWVAVAVLLAGACVHVVGEMIGSGG
                     QWALQMGLAPHERQGQYQGFASLGFSLMNVVGPPVVTLLCIQGGRLGWVAMGLIVLVS
                     GVAAQALGRWALANRTAYGVTTHSG"
     gene            5382..6158
                     /gene="ftsE"
                     /locus_tag="PLK64_04580"
     CDS             5382..6158
                     /gene="ftsE"
                     /locus_tag="PLK64_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018768470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HPV79050.1"
                     /translation="MILFDAVTKTYPKATRPALSEVSLEVERGEFVFIVGSSGSGKST
                     LLRLAIREERATSGAVLVAGRELRTLPDRRVPMLRREIGTVFQDFRLLPNKSVFQNVA
                     FALQVIGKSRALIRQTVPETLRVVGLEGKEKRLPHQLSGGEQQRVAIARAIVNKPTIL
                     LADEPTGNLDPATSRAIVELLDRINRSGTTVVVATHDHEMVNEFRRRVIELDAGKLVR
                     DEADGDYGKTTSAIPVVRIDEGDSDAEDGTPDAGSAGGER"
     gene            6158..7066
                     /gene="ftsX"
                     /locus_tag="PLK64_04585"
     CDS             6158..7066
                     /gene="ftsX"
                     /locus_tag="PLK64_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HPV79051.1"
                     /translation="MRAGFVLGELGTGLRRNLSMAVSVILVTMISMYLLGLGLLAQRQ
                     VDTMKDYWYDRVQVSIFLCVEGSQYAACSGKATTPTQREQIKAQLEQMKPPVKEIFYE
                     SQADAFKRFKEQFKNSPLAEQATERAFADSFRVSLTDAGKYDVIVSSFSGAPGVGRVQ
                     DQKQLLDRFFAFMNAVSWGAIALSALMVLCAVLLMATTIRQTAFSRRRETGIMRLVGA
                     SNATIQLPFVLETLVSALVGASLAMGLLWATVSYGIGGYLSKALMDTAFIGMTDVFAV
                     APWVVGGVAIVAVLTSWFTLRRYLRV"
     gene            7183..8526
                     /locus_tag="PLK64_04590"
     CDS             7183..8526
                     /locus_tag="PLK64_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPV79052.1"
                     /translation="MLTSPPGRRPGLRAGAAGLVVLSFSAVLLAPPSAADTGDEKRAA
                     DAQVADLEEQLSETSAELAQAYTALTQTQAQLPAARARLASAQSAARAAAKHNAEVAA
                     ALAVAQANAARAEEAVARNTATRDQTQRTLDAFAADLFQGGSVGQLSVALGATSADDF
                     ATRLVLADTVTAMTNQALNDLAAAKADGDANGAYLAAVQVEVTDLKRQAENALAAAEA
                     SRNEAQAASSALDALVAQQSGYAASVEAQRANEASQLAEAQAEQQRLQALLIEQARIA
                     REREAARKAAEAAAQKAAEEAARKNGTAVPPPPVAQPDQASGFLSRPITGGRTSSEFG
                     MRFHPIFQVWRLHTGLDFANGCSSPVYAAAPGTIIRAGWAGGRGNQIVIDHGIRRGVD
                     LATTYNHLTSFVQRSGTVSRGQLIGYTGTTGNSTGCHLHFETLEDGQFVNPRSWL"
     gene            8608..9078
                     /gene="smpB"
                     /locus_tag="PLK64_04595"
     CDS             8608..9078
                     /gene="smpB"
                     /locus_tag="PLK64_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPV79053.1"
                     /translation="MVKESGRKVVASNRKARHDYHIEDTFEAGISLMGTEVKALRAGR
                     CSLVDGFAQFTGDELWLEGVHIPEYSQGTWTNHAPRRRRKLLLHREELTKIESKIREK
                     GRTIVPLAVYFKDGRAKVEIALATGKRQYDKRQALRERQDKREADRAMHSRSER"
     gene            9075..11126
                     /locus_tag="PLK64_04600"
     CDS             9075..11126
                     /locus_tag="PLK64_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HPV79054.1"
                     /translation="MSHPARSHRHRRGYAARLALLVGAALTLLGVAAAPAALAQTPAQ
                     SSTTAAVAPWSDLSAAPALAALATADRMPAFDSAFVVDKDGGITVTETIQYSFDSSGG
                     VRHGIYRAITVRQGVDGRTDVYRYLSMTGVSVSSPTGADATVQLVDNGPDVTIRIGSA
                     SKTVSGTQTYVVRYHLANVLNPFTDTQTAEFYYNVIKDDTVAKGAVTVSVTAPVAATD
                     VRCTRGVGAACDSATGGATARFAVGSLAAGENLTIDTRYPLSGFSKIAPDIRTGTAGA
                     PGSGLDEGVAKVASLASVAGGVLAPIVALVVMGMLVATRGRDEWYAGLTPGLTPGLGQ
                     GPIADGIPPGGSAPTVRGKTPTIAVQFNPPPGVQPGLVGTVIDESADTLDVSATVIDL
                     AVRGYLRIEEVEGGGMFARTDWRLTALSPTTAHELRPYEQSVLVGLFQQGSPVLLSDL
                     KNHFAKTLAATKDLMYDEVLSRGWFRKSPQSQRTAWRGFGFFVMGAGIVTIFAYGSIS
                     RAIDISSGLPLPIPSGWVLGAGLLLAGFVIQLLGARMAAKTAAGSAVLAQSLGFRQYL
                     VTAEANQIRWEEAQDVFSRYLPFAIVFGVADRWAKTFQEVAAAAAAAGQVVIMPDWYI
                     YHGALFPDFGSIVDGVDSFSTTASGTFQSTPGSSGGSGFGSSGGSFSGGGVGGSSSGS
                     W"
     gene            complement(11156..>12914)
                     /locus_tag="PLK64_04605"
     CDS             complement(11156..>12914)
                     /locus_tag="PLK64_04605"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018771497.1"
                     /note="biotin-containing enzyme that catalyzes a two step
                     carboxylation of pyruvate to oxaloacetate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HPV79055.1"
                     /translation="ALTPQLLSVEAWGGATYDVALRFLAEDPWDRLAALRAAMPNLPL
                     QMLLRGRNTVGYTPYPTTVTDAFVAEAAATGIDIFRIFDSLNDVSQMRPAIDAVRATG
                     TAVAEVALCYTSDLNDPNERLYTLDYYLHLAEAIVAAGAHIIAIKDMAGLLRAPAARR
                     LVTALRERFDLPVHLHTHDTAGGQLATLLAAIDAGVDAVDVASSSMAGTTSQPPMSAL
                     VAATNATERATGLDLAAVCSLEPYWEAMRTLYRPFESGLPSPTGRVYTHEIPGGQLSN
                     LRQQAIALGLGDRFEAIEDMYAAANKILGNIVKVTPSSKVVGDLALALVGAGADPAAF
                     EADPTAYDIPDSVIGFLGGELGDPPGGWPEPFRTKALAGRTIKRAETELAPDDLAALA
                     VSPRATLNRLLFPGPTKEFLASREAYSDLSIVGTVEFLHGLVPGEEHSIDIEPGKRII
                     VSLQAVSEPDERGFRTVMCTLNGQLRPVQIRDTSVKVDVRQAERADPAIKGHIAAPFA
                     GMVTPAVREGDTVAAGSVVATIEAMKMEASITTPVGGVVERLAIGGHTQVDGGDLILV
                     VQVGQPPAPKAPEIEASAG"
BASE COUNT         1989 a         4369 c         4513 g         1943 t
ORIGIN      
        1 tctggatcgc cgccggccct gcgctggtcg acctgttcca gagttccctg tcgagtgtca
       61 ccggcaagaa gtagcccgat ggccgcggtg gcggcgaccc ggccgggtga gcgtggctcg
      121 gcggtcgccg agttcgtcat ggtctctacg ctcgtcctgg tcctcggcat gggggtcttc
      181 caactgggcc tcgtcctgca cgtgcgcaac accctcatcg cctgcgccgc cgaaggagcc
      241 cggctcggcg ctcgggccga cgtgcccacg tcggacgcca gtgagcgaac tggcgcgctc
      301 gtgaccgaca ccctgtcggc ggcatacgcc caggacatct cgacgcggcg gacggtgacc
      361 gacgcgggag tccgagtcgt cgaggtcacc gtcgttgccc ccctgcccgt gatcggcctg
      421 atcgggccgg gcgggtcgat gacggtggtc gggcgggcgt tcgacgagcg ccaggtgatc
      481 gagccatgac caggctcacg gggctcgttc ggtgggtgcg gctgcgggtc ggccgccccg
      541 accaggggcg agcaatcgtc gagttcgtct tcctcggggt gctgttgctg gtgccgctca
      601 tctaccttgt catgacgctg gcggtgctgc aggcggcggc atactcagcg acaacggcgg
      661 ctcgcgaggc gggtcgcgcc ttcacgacgg ccccggcgga gagccaaggt atgccgcgcg
      721 cccgggccgc ggcccaggtc gccttcgaag acttcgggtt cctgcccgag gagacttcgg
      781 tggcgatcac gtgcgacggc tctccctgtc tgcgcccgga cgcgcgggtc agcgtgacga
      841 cgtcgatccg ggtgcgactg ccctaccttc cggcgttcct cggcgatgcc gtccccgccg
      901 cgattcccgt gtcggccacg catgtggcca ccgtcggtcg gttcggtggg tcatgagcgg
      961 gccgaggcgg gtcagtgcgg cggtgcggcg gttcagggcc gcggtgcggc ggcgccgggc
     1021 cgagtcgggg caggtgtcgg tgctcatcct cggcatggtc atgctcgcga tgctgctgat
     1081 cgtcggggga gtggatgtca ctgccgcgca actggctcgg gtgcggttgg tcgatgcggc
     1141 cgacgcagcg gcgttggatg cggctgacgc gttggacgag gcgacggcat accgcagcgg
     1201 gttgtcggac gcggtggtgc tcagttcggc gacggtgaag gaggctgcgg cggcatacct
     1261 ctcggtgcgg cccaagcccg agggggtgcg ggcgtggggg atcgcgccgg ggacgggcgc
     1321 tcagggaggc gacaccgcgg tcgtcgtcct ggatgcgatc gtcgagttgc cgatgaccgg
     1381 cggactgctc gcggcgctcg gcaaggagat cgcggtgcac gtcgaggcca aggcccgcgc
     1441 cccgctcctg cctgtgccct agatacgacg tgcgccgcgg cgcccgaggc cggtcaaccg
     1501 gcgctgtgcg gagtgaaggc gaccctctgg tgtggccaac actccgcaca ggccacctca
     1561 ggggcggcat acctcaacca ggctcagcgc agcaggtcgg cgacctcggc ggaggcgtag
     1621 aagggctcaa gctgggcacg gagcagccgt tcgaggtctc cgctggcgcg cagggtgtcg
     1681 accacgaacg aagccagcgg cacgaggtgg gcgatgacct cgtcgcgcgg ccattcgagt
     1741 tggtcgagca gctcagccag ggtgtagccc ccgaaccact cgaagagggc gtcgaccacc
     1801 agcgtggcga cgcgggcggc gtgctcggtg ccgagggcgg tcacggcgag ttcgtgggcg
     1861 gcgtcgacca cgccggagac ctccgcgtgc gcggcatacg ctccgagtcc gatgacccgc
     1921 tcgccggagg tctggtccca gacctgcagg atcgcctcga gggtcgtggg gtcgcgcaga
     1981 gtctcgacga agatccggtt gaggcgccgg accgcgaagg tgccgccctt gccgacggcg
     2041 tcgccgatca gcccttcgaa ctgccggtcc gcagcacccc gcagccgcga tgccgtgctc
     2101 gtcccgaagg acaccagcgg ccctagccca ggcaccttgg acgcgaccgc ctggttggcc
     2161 tggatcacct cgctggcgat ccggcccatg aaccgcgacg cgacnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnncccggt cgatcaggtc ggcgagttgg tgcggcccgg
     2341 cgctgtccag gaggctctcg acgagcccat caaccgtgga ctcgagccgg tcgccggtga
     2401 accgatcgag gtggtaggcc acctgggcgt cgagcagccg ctgggcgagg gtgtctgctc
     2461 gcgacgcacg gggaggcatg gccccacgct agaccgcgcc cgcggcgagc acacggcata
     2521 ccaatggtcg ggtgggatgc ggcgcctgag cacgggccgc gcccgcctcg ccgaccgatg
     2581 gccttcggtg tgcgcagggg tatgccggtc gcccgcctcc cagccccggt gcggcgcggg
     2641 tgtggctccg acgtggggca cgatcggtga gcggcgcggc atacaggggg cctcgcgtag
     2701 gcttgaccct cgtggctgtc gacttcggag aagagatcaa gcacctgcgc gcgacgatgg
     2761 attccgtgcg cgaggtgacc aacctcgatg ctctgacgac cgagatcgcg cggctggagg
     2821 cggaggcgtc ggtccccaac ctctgggacg accccgaccg ggcgcagcag gtgacctctg
     2881 cgctgtcccg cgccaactcc gagcgcgaac gggtgaccgg gatggacagc cgggtcgaag
     2941 acctcgaggt gctcgtcgag atgggcaccg aggagggcga cgccgacacc ctcgccgagg
     3001 ccgagcgcga tctggccaag cttaagaagg acgtcggtga gcttgaggtc cgcaccctcc
     3061 tcaacggtga gtgggacgcc cgcgaggccg tcgtcaccat ccgctcgggt gctgggggag
     3121 tggacgcggc cgacttcgcc gagatgctca tgcggatgta cctgcgctgg gccgagcggc
     3181 acaactaccc gacaacggtg ctggacacct cctacgccga ggaggctggc ctcaagtcgg
     3241 cgaccttcga ggtcaaggtg ccctatgcct tcggcaacct ctcggtcgag gcgggcaccc
     3301 accggctggt gcggatcagc ccgttcgaca accaggggcg ccggcagacg tcgttcgcag
     3361 cggtcgaggt ggtcccgctc atcgagcaga ccgaccacat cgacatcccc gagaacgagc
     3421 tcaagatcga cgtgttccgc tcgtccggcc ccggcgggca gagcgtcaac accaccgact
     3481 cggcggtccg gatgacccac atccccacgg gcatcgtcgt ctcgatgcag aacgagaagt
     3541 cccagatcca gaaccgagcc gccgccctgc gcgtcctgca gtcacgcctg ctgctgcaga
     3601 agaaggccga ggaggccgcc acccgcaagg agatggccgg cgacgtcaag gccagctggg
     3661 gtgaccagat gcgttcctat gtcctgcagc cctatcagat ggtcaaggac ctgcgcaccg
     3721 accacgaggt cggcaacccg tcggcggtct tcgacggcga catcgacgac ttcatcgagg
     3781 ccggcatccg ctggaagcgc aacctggaga agggcaacgg ctgaccgcgg ccgtaagtct
     3841 cagcctccgc tcgccgatca gcccgcacgc accactcagc ccgagtgggt cgtcactccg
     3901 tatgccgtgc ggttggccag agcccagcgc cccagcgcct gcgcggcgac accagagacc
     3961 aggacgatca ggcccatcgc gacccacccc aggcggccgc cctggatgca cagcagcgtg
     4021 accaccggcg gcccgacgac gttcatcagc gagaagccca ggctcgcgaa cccctgatat
     4081 tgcccctgcc gttcgtgcgg ggccagcccc atctgcaacg cccactggcc gcctgagccg
     4141 atcatctcgc cgacgacgtg gacgcaagcg ccggcgagca acacggccac cgccacccat
     4201 gccgagctgc cgtcggccag cccgatgagg gcgaacccga ccgcgatgag cgcgccgccg
     4261 cgcaccaacg cccgccctcc agtggcgacg gagtcggcgg aggtggacag gcggacctgc
     4321 agcgaggcga ccgcgaccgt attgaccagc aggagcactg cgaccatcac ttggggcgcc
     4381 tgggtccgct gcgacacgaa cagggccaga cccaactcgg cggcgacgaa gtgcacggag
     4441 aacattgacg tgctcagcac gactgcgacg aacggcaggt cgcgcagcac cccgagccgc
     4501 ggctcaccgt ccggtcggcg gtagggcggc aggtgggtca gccgggtggt gttccacgca
     4561 gcgaagccgc acagcacgcc ggccatcacg aagacgctca cgtatgccgc gcgggtgtcc
     4621 acgacgagcg ccgccccgcc cacgaccgag cccagcgcga tgccgctgtt ggtcacggca
     4681 cgcagatagg ccttgaactg cacgcctcgc ccgccgatcg ccacctgggc ggtgaggcca
     4741 ttgcggaccg cgttggcggc ggaggccagg gcggcctcga ccgccagcag cagggtgagc
     4801 acccacggcg aggttgccag cacgggcaac ggcgtcgcga ccgcgagggc gagcatgatc
     4861 acgttgagca cctcgcgggg gccgcgggtg tcgcccagat gcccagcggg aagggccgcg
     4921 atcatcgcgg ccgcggctgc caccgagacg gccagcgcca cctccccgac agtgaaaccc
     4981 aggtggcgag tgaagtagat ggccgaggtc gtcatcaccg caccgctgcc gaagcgggtc
     5041 gcgaacgctc cgatggccag gacgcgcagt tggggatcga ggggtatgcc gcgtcgcgtc
     5101 agcgcctcac cctcgcctgc ggcggccatc gggttccccc tccaagtatc ttgacatcaa
     5161 gatacttccc tacgcccgac gagtgcaaac cgggcgcaat cgggtacaac ccggccgcgg
     5221 ccagaggggg agggcgcgag tcggggcctt gcggcatacc cctcgcgcca cgccagccac
     5281 gtgcataact gtggtttctc cagtcactca actaccctcg gaccacacca gggctcgcgc
     5341 aggccctggc cccaggctgc cctgaccgga gaaaccgacg catgatcctg tttgacgccg
     5401 tgaccaagac ctaccccaag gccacccgcc cggccttgtc cgaggtctcc cttgaggtcg
     5461 agcgaggtga gttcgtcttc atcgtcggga gttccggctc gggcaagtcg acgttgttgc
     5521 gcctggccat tcgcgaggaa cgggcgacgt cgggcgccgt cctcgtcgct ggccgcgagc
     5581 tgcgcacgct gcccgatcgt cgggtgccga tgctgcgccg cgagatcggg acggtcttcc
     5641 aggacttccg gctgctgccc aacaagagcg tcttccagaa cgtcgccttc gccctgcagg
     5701 tcatcggcaa gtcgcgcgcc ctcatccgcc agaccgttcc cgagacgctg cgcgtcgttg
     5761 ggcttgaggg caaggagaag cggttgcccc accagctctc tggtggtgag cagcagcggg
     5821 tggccattgc tcgggccatc gtcaacaagc cgacaatcct gttggccgac gagccgaccg
     5881 gcaacctcga cccggccacc agtcgcgcga tcgtcgagtt gctcgaccgg atcaaccgct
     5941 ccggcaccac ggtcgtcgtg gctacccacg accacgagat ggttaacgag ttccgccgcc
     6001 gggtcatcga gctcgacgcc gggaagttgg tgcgcgacga ggccgacggc gactacggca
     6061 agacgacctc ggcaatcccc gtcgttcgaa tcgatgaggg cgactcggac gccgaggatg
     6121 ggacccccga tgccgggtcg gccggagggg agcgctgatg cgggccggat tcgtcctcgg
     6181 tgaattgggc accgggctgc gtcggaatct gtcgatggcc gtgtcggtca tcctcgtcac
     6241 gatgatctcg atgtacctgc tcggactcgg gctcctggcc cagcgccagg tcgacaccat
     6301 gaaggactac tggtacgacc gcgtgcaggt ctcgatcttc ctgtgcgtgg agggctcgca
     6361 gtatgccgcg tgcagcggta aggcgaccac ccccacccag cgtgagcaga tcaaggccca
     6421 gctcgagcag atgaagcctc cggtcaagga gatcttctac gaaagccagg ccgacgcgtt
     6481 caagcggttc aaggagcagt tcaagaactc cccgttggcc gagcaggcga ccgaacgggc
     6541 cttcgccgac tccttccggg tgtcgttgac cgacgcgggg aaatacgacg tcatcgtgtc
     6601 gtcgttctcc ggtgccccag gagtcgggcg ggtccaggac cagaagcagt tgctcgaccg
     6661 attcttcgcg ttcatgaacg ccgtgtcgtg gggggcgatc gccctctcgg cgctcatggt
     6721 cctgtgcgcc gtcctgctca tggccacgac cattcgccag acggcatttt cgcggcggcg
     6781 agagaccggg atcatgcggc tggtcggtgc gtccaacgcg acgatccaac tgcccttcgt
     6841 gctcgagacg ctcgtctcgg ccctcgtcgg agcctcgttg gcgatgggcc tgttgtgggc
     6901 gaccgttagt tatggcatcg ggggatacct cagcaaggcg ttgatggaca ctgcgttcat
     6961 cgggatgacg gatgtcttcg ccgtggctcc ttgggtggtc ggcggggtgg cgatcgtcgc
     7021 ggttctgacg tcatggttca ccctcaggcg ctaccttcga gtgtgacggg ggtcggggga
     7081 tgcaaggtcg attggtcagg tttcacaggt gagcccgctc atctgtgccg accgagtgtg
     7141 gtaccaaagg gactgatgat tctccagaga ttgatgcgac gggtgttgac ttcacccccc
     7201 ggccgccgcc caggcttgcg ggctggcgcc gccggtctcg tcgtgctgtc cttctctgcc
     7261 gtgcttctgg cccctccgtc ggctgctgat accggggatg agaagcgtgc cgccgacgcc
     7321 caggtcgccg acctggagga gcaactgagc gagacctccg ccgagttggc gcaggcctat
     7381 acggcgctca ctcagactca ggcgcagttg cccgccgccc gggctcggtt ggcctcggcg
     7441 cagtccgcgg cgagagcagc ggccaaacac aacgcagagg tggcggcagc cctggcggtg
     7501 gcgcaggcga atgccgcgcg cgccgaggaa gcggtggccc gcaacacggc cacccgcgat
     7561 caaactcaac gcacgttgga tgccttcgcc gcagacctgt tccagggcgg ctcggtgggc
     7621 cagctgtccg tggctctggg cgccaccagc gccgacgact tcgccacgcg actcgtcctg
     7681 gccgacacgg tgacggcgat gaccaaccag gctctcaacg acctcgccgc agctaaggcc
     7741 gacggggacg ccaacggcgc ctacctggct gcggtgcagg tcgaggtcac cgacctcaag
     7801 cggcaggcgg agaacgcctt ggccgccgcg gaagcctcgc gaaacgaggc gcaggcagca
     7861 agttcggccc tcgacgcgct cgtcgcccaa cagagtgggt atgccgcctc ggtcgaggcc
     7921 cagcgcgcca acgaggcgag ccaattggcc gaggcgcagg ccgaacaaca gcgcctgcaa
     7981 gccctgctca tcgagcaggc ccggatcgcg cgggagcgtg aggcggcccg caaggccgcg
     8041 gaagctgctg cccagaaggc cgccgaggaa gccgctcgca agaacggcac ggcggtcccg
     8101 ccgcctcccg tcgcgcaacc cgaccaggcc tccggcttcc tcagccggcc gatcaccggt
     8161 ggtcgcacca gctcggagtt cggcatgcgt ttccacccga tcttccaggt ctggcggttg
     8221 cacaccggcc tcgatttcgc caacggctgc agttcgcccg tgtatgccgc cgcgcccggc
     8281 acgatcatca gggccgggtg ggccggtggg cgaggcaacc agatcgtcat cgaccacggg
     8341 atccgccggg gtgtcgacct ggccacgacc tacaaccacc tcacgtcctt cgtccagcgc
     8401 agcggcaccg tgtcgcgcgg tcagctcatt ggctatacgg ggacgaccgg gaactccacc
     8461 ggctgccacc tgcacttcga gacgttggaa gacggccagt tcgtcaatcc gcgctcttgg
     8521 ttgtaggcgt gcttaaagcc ttcccggccg caggtgccga cggggtagcg ttggctctcc
     8581 ggtaggtgac agggaggacc cactcacgtg gtcaaggaaa gtggccgaaa ggtcgtcgcg
     8641 agcaaccgca aggcccgcca cgactaccac atcgaggaca ccttcgaggc gggcatctcc
     8701 ctcatgggca ccgaggtgaa ggcgttgcgt gccgggcgct gctcactggt cgacggcttc
     8761 gcccagttca ccggcgacga gttgtggctc gagggtgtcc acatccccga atacagccaa
     8821 ggcacctgga ccaaccacgc ccctcggcgc cgccgaaagc tcctcctgca ccgcgaggaa
     8881 ctcaccaaga tcgagtcgaa gatccgcgag aagggccgca cgatcgtccc cctcgccgtc
     8941 tacttcaagg acggccgcgc caaggtcgag atcgccctgg ccaccggcaa gcggcaatac
     9001 gacaaacgcc aggcccttcg cgaacgccag gacaagcgcg aagccgaccg cgccatgcac
     9061 tcccggagcg agcggtgagc cacccggctc gctcgcatcg acaccgtcgg gggtatgccg
     9121 cgcggctcgc cctgctggtg ggcgccgcgc tcactctgct cggcgtcgcg gcagccccag
     9181 ccgccttggc ccagacaccg gctcagagtt caacgacggc cgcggtcgca ccctggtccg
     9241 acctctcggc cgctcccgcg ctggctgctc tggcgacggc tgatcggatg cccgctttcg
     9301 actcggcgtt cgtcgtcgac aaggacggcg ggatcacggt caccgaaacg atccaataca
     9361 gtttcgactc ctccggcggc gtccggcacg gcatctaccg ggcgatcact gtgcggcagg
     9421 gggtcgacgg gcgcaccgac gtctatcgct acctcagcat gacgggggtc agcgtctcca
     9481 gcccgacggg cgctgacgcc acggtccagt tggttgacaa cggccccgac gtgacgatcc
     9541 ggatcggctc cgcctccaag acggtgagcg gcacgcagac ctacgtcgtg cgctatcacc
     9601 tggccaacgt gctcaacccg ttcaccgaca cccagacggc cgagttctac tacaacgtca
     9661 tcaaggacga cacggtcgcc aagggtgcgg tcaccgtgtc ggtgacggct ccggtcgccg
     9721 cgaccgacgt gcggtgcacc cgcggggttg gcgcagcctg cgactcggcc accggtggcg
     9781 ccacggcgcg attcgccgtg ggcagcctgg cggcggggga gaacctcacc atcgacacgc
     9841 gctatccctt gtccggtttc tccaagatcg cgccggacat ccgcaccggc accgccgggg
     9901 ctcccggctc gggcctggat gagggtgtcg cgaaggtcgc ctcgctcgcg tcggtggccg
     9961 gtggcgtcct cgcccccatc gtcgcgctcg tcgtcatggg gatgctcgtc gccacccgtg
    10021 gccgggacga gtggtatgcc ggtctgaccc caggtctgac cccgggcctc gggcaggggc
    10081 cgatcgcgga cggcataccc cctggtgggt ccgcgcccac ggtgcgaggg aagactccga
    10141 cgatcgcggt gcagttcaac ccgccgccgg gagtgcaacc gggcctggtc gggacggtga
    10201 tcgacgagtc tgccgacacg ctcgatgtgt cggcgacggt catcgacctc gcggtccggg
    10261 gctacctgcg catcgaggag gtcgagggcg gggggatgtt cgcccgcacc gactggcggt
    10321 tgacggccct gtccccgacg acggcgcatg agttgcgccc ctacgagcag agcgtgctcg
    10381 tcgggttgtt ccagcagggc agcccggtgc tgttgtcgga cctgaagaac cacttcgcga
    10441 agacgctggc cgcgaccaag gacctgatgt acgacgaggt gctttcgcgc ggttggttcc
    10501 gcaagtcacc gcagagccag cggacggcct ggcgtggttt cggcttcttc gtcatgggcg
    10561 ccgggatcgt cacgatcttc gcctacggct cgatctcgcg ggccatcgac atctccagcg
    10621 ggctgcccct gccgattccc tcgggttggg tgctgggggc cgggttgctg ttggcgggct
    10681 tcgtcatcca gttgctgggt gcccggatgg ccgccaagac cgccgccggg tcggccgtgc
    10741 tcgcccaatc gctgggcttc cgccaatacc tcgtgaccgc cgaggcgaac cagatcaggt
    10801 gggaggaggc gcaggacgtc ttctcgcgct acctgccctt cgccatcgtc ttcggggtcg
    10861 ccgaccgttg ggccaagacg ttccaggagg tcgcggccgc tgccgcggct gccggccagg
    10921 tcgtcatcat gcccgactgg tacatctacc acggcgcgtt gttccccgat ttcgggagca
    10981 tcgtcgacgg ggtcgactcg ttctcgacga ccgccagtgg gacgttccag tcgacgcccg
    11041 gctcgtccgg cgggtccggg ttcggcagtt cgggcgggtc gttctccggt ggcggggtcg
    11101 gcggctcgtc ctccggctcg tggtgagccg gaggacctga cccgacgccg gcgggctagc
    11161 ccgccgaggc ctcgatctcg ggagccttgg gtgcgggcgg ttgaccgacc tggacgacga
    11221 ggatgaggtc tccgccgtcg acctgggtgt gtccgccgat ggccagccgc tcgacgacgc
    11281 cgccgaccgg cgtggtgatc gaggcctcca tcttcatggc ctcgatggtg gccacgaccg
    11341 agccggctgc gacggtgtcg ccctcgcgga ccgcaggcgt caccatgccg gcgaagggcg
    11401 cagcgatgtg gcccttgatc gccgggtcgg cgcgctcggc ctggcggacg tcgaccttga
    11461 ccgacgtgtc gcggatctgc acgggccgca gttggccgtt gagggtgcac atcaccgtcc
    11521 ggaaacctcg ctcgtcgggc tcggacaccg cctgcagcga gacgatgatc cgcttgccgg
    11581 gctcgatgtc gatgctgtgt tcctcgccgg gcacgaggcc gtgcaggaac tcgaccgtcc
    11641 cgacgatcga caggtcggag taggcctcgc gggaggcgag gaactccttg gtcggcccag
    11701 ggaacagcag ccggttgagg gtggcccgcg ggctcacggc gagcgccgcc aggtcgtcgg
    11761 gtgccaactc ggtctcggcc cgcttgatcg tgcgcccggc cagcgccttg gtccggaacg
    11821 gctcgggcca gccaccgggc gggtcaccca gctcgccgcc gaggaagccg atgaccgaat
    11881 cggggatgtc gtatgccgtg gggtcggctt cgaaggcagc cgggtcggca cccgctccga
    11941 cgagggccag ggcgaggtca ccgaccacct tggaggacgg cgtcaccttg acgatgttgc
    12001 cgaggatctt gttggccgcg gcatacatgt cctcgatggc ctcgaagcgg tcgccgagcc
    12061 cgagggcgat cgcctgctgg cgcaggttgg acagctgacc gccggggatc tcgtgggtgt
    12121 agacccggcc ggtgggggag gggaggcccg actcgaacgg ccggtagagc gtgcgcatcg
    12181 cctcccaata gggctcgagc gaacagacgg ccgcgaggtc gagcccggtc gcgcgttcgg
    12241 tcgcattggt cgcggcgacc agggccgaca tcggcggctg gctcgtcgtg ccggccatcg
    12301 acgacgaggc gacgtcgacg gcgtcgacac cggcatcgat cgccgcgagg agcgtcgcga
    12361 gttgaccgcc ggccgtgtcg tgcgtgtgca ggtggaccgg caggtcgaac cgctcgcgca
    12421 gcgccgtcac caggcgacgg gccgcggggg cgcgcagcag cccggccatg tccttgatgg
    12481 cgatgatgtg cgccccggcg gcgacgatcg cctcggccag gtgcaggtag tagtccagcg
    12541 tgtagagccg ctcgttgggg tcgttgaggt cggaggtgta gcacagggcg acctcggcga
    12601 cggcggtgcc ggtggcacgc accgcgtcga tggccggacg catctgggac acatcgttga
    12661 gtgagtcgaa gatccggaag atgtcgatgc cggtcgctgc tgcctcggcg acgaacgcgt
    12721 cggtcaccgt cgtcgggtag ggcgtgtagc cgacggtgtt gcggccacgc agcaacatct
    12781 gcaacggcag gttgggcatg gcggcacgca aagcggccag gcgatcccac gggtcttcgg
    12841 cgaggaagcg cagggccacg tcgtaagtcg cgccgcccca ggcctcgacc gagagcaact
    12901 gcggggtcaa cgcc
//
LOCUS       DAOMZY010000052        12865 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_4497_length_12865_cov_5.421311, whole genome shotgun sequence.
ACCESSION   DAOMZY010000052 DAOMZY010000000
VERSION     DAOMZY010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 12865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12865
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4497_length_12865_cov_5.421311"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PLK64_04610"
     CDS             complement(<1..615)
                     /locus_tag="PLK64_04610"
                     /inference="COORDINATES: protein motif:HMM:NF014998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HPV79056.1"
                     /translation="MQQLTGLDEMFLSLDTGRTTGHVAGIAFFDKPAEKRDELAFLRE
                     RVAERLTRLPVLRWRLKSVPLEMDGRYWVDGPVDIKEQVIGIRLPKPGTDAQLHDAID
                     RIMAQLLERDRPMWRIYVIEGLEDGRYAYVVKMTHGLADGSVLWMLFDQLSDHPTEVL
                     PEVPMSAEPRGGKAEMLARGLIGLAKKPVKGIKLQAETAAWAAGK"
     gene            complement(808..3618)
                     /locus_tag="PLK64_04615"
     CDS             complement(808..3618)
                     /locus_tag="PLK64_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPV79057.1"
                     /translation="MSELTRFAAGLDFGLDPFQVAGCEALEAGRGVLVAAPTGAGKTV
                     VGEFAVHLALATGRKAFYTTPIKALSNQKYADLCRRHGAANVGLLTGDSSVNGEAPVV
                     VMTTEVLRNMMYAGSPTLAGLGYVVMDEVHYLADRFRGAVWEEVIIHLPEDVLVVSLS
                     ATVSNAEEFGEWLGEVRGDVAVVVSEIRPVPLWQHVMVGKQLFDLFVEESLDSSGRPQ
                     VGRRVQVNPELLERIRSVERALAADSAAHRGLRGRTGPGGGPPGGGRGPRGGRGSWES
                     DAPARGREGGIRPGGGPTRADVIATLDREGLLPAITFVFSRNGCDAAVGQLLARGIRL
                     VSEADGVRNRRYVEERIGALADEDLSVLGYWDFVDGIERGFAAHHAGMLPTFREIIEE
                     LFTQGRVRAVFATETLALGINMPARTVVLEKLVKYNGDTHAELTPAEYTQLTGRAGRR
                     GIDVEGHGVVLWSRGLDPLVVGGLASTRTYPLRSSFRPTYNMAVNLVAQVGRDRAREL
                     LETSFAQFQADRSVVGLAQSVRRGERQLAEYAVEWHCHLGDFGEYAALRASLRDLEKG
                     AAKARSAATRAEAELSLERLRPGDVIRVPTGRRAGYAVVVAANRGAPGRPASPAVVTE
                     DRQLRRLTTVDVPTPVQPLLTVRIPTGFNAKSPKSRRDLAATLRIAVAHEPPPRHTSR
                     GGDDDARRESDAEVTRLRAALREHPCHACPDREEHSRRAEKWWRLRRETDALARQVDG
                     RTNTVARTFDRICDLLEDLGYLGEGGLTVTEAGQDLRQIYAERDLLAAQCIREGVWNR
                     LDAAGLAAVVSALIHEPRREDLAAAPRVPTTDVGEALTAMTALWATLEEQERLHGLPP
                     SGAPDAGIVWMVHRWASGQRLETVLRGSDLAAGDFVRRCKQVVDLLGQISDIAPHPAL
                     SAKARKAVDAVRRGVVAADRID"
     gene            complement(3615..4523)
                     /locus_tag="PLK64_04620"
     CDS             complement(3615..4523)
                     /locus_tag="PLK64_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HPV79058.1"
                     /translation="MTHFTGPLRRIGLYANPVSGRGRSETAAAQLRGILADSGHIVLD
                     IAAQSAAEGRVRTFDAVRSGAIDALVVAGGDGTANLGANACAGTDIPLVVLPAGTGND
                     NARSLGMPRKDIPAIARLISDGQTRRIDAGRCVTADGELWWLGVLGGGFDTLVNTRGR
                     SLTALHGTPRYLAAVALELPRFTGIPYVVQVDDQRIETEAMLVAVANGGTFGGGMKVC
                     PDASMTDGLFDVMILHKIGRGEFLRIFPRVFSGGHVSHPAVEILRGRRVHLEADGIDS
                     QADGEDFLPLPVDLEVVPGALAVVAP"
     gene            complement(4533..5375)
                     /gene="tatC"
                     /locus_tag="PLK64_04625"
     CDS             complement(4533..5375)
                     /gene="tatC"
                     /locus_tag="PLK64_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HPV79059.1"
                     /translation="MPSRTPMARFRFRRNRDPEGHMSLGDHLRELRRRTVISALAIVV
                     GAVFGWTRWVAIYDALTSPVTEVARTKTAGALTTVSFSTPTDSFSVMITVGLFVGVVV
                     ASPVWLWQIWAFIVPGLTRKEKRITVAFIGAAVPLFLGGCWLAYAVLPRAMGALLSFT
                     PTSGSNIIDVSLYLSFVLKFILVFGLGFLLPVFLVGLNVAHILPGRLMLRAWRGAVFM
                     CFLFTAVMTPDPNPWTMVILAVPLVMLYFAAVGVSLLLDRRRARSDPSAEWLRVADDE
                     ASDL"
     gene            complement(5427..5666)
                     /gene="tatA"
                     /locus_tag="PLK64_04630"
     CDS             complement(5427..5666)
                     /gene="tatA"
                     /locus_tag="PLK64_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012245431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HPV79060.1"
                     /translation="MFLREPSHWLIVVLALVLLFGWKRLPDMARSVGRSMRIFKSEVN
                     EMKNDGKEGRPSPAADDVVPGDVVPPRSPDDRPSV"
     gene            complement(5672..6028)
                     /locus_tag="PLK64_04635"
     CDS             complement(5672..6028)
                     /locus_tag="PLK64_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79061.1"
                     /translation="MRGLVWTLIWLALVAGAAWYLWRRLRWLWGRTEMLGDTLAEAET
                     LFAAATEREVDPRDGVTDHRPQVELAIFRAPADVAQERDLIRETLRGERAARRRASLP
                     GWARRVDSIDADHRKA"
     gene            complement(6028..7089)
                     /locus_tag="PLK64_04640"
     CDS             complement(6028..7089)
                     /locus_tag="PLK64_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HPV79062.1"
                     /translation="MTATRAGRPVQESATARLSRLLTMVPWLLARQGVDVDVAAAQFG
                     VSREQLESDLALLFVCGTPGHLPDDLIEADWEDGQIYVGNADAISRPLRFTVDEALTL
                     MVGLRALAATPGVTGASGADGGEAILRALTKLEQATGEAAGVAARVRVTLDDGAAETI
                     LADASRAVQERRRVRLSYQSFGRDEVTEREVDPMRVTSLDARWYLEGWCHRAEDVRLF
                     RLDRVQSLQVLDVDGTPPESAQLRDLAGETYQPSPDDVLVELLVEQAAAWVADYYPVD
                     SVTDAGDGRRRILLRAADPSWVRRLAWRLGGHVQILAPQSLAEQVAAGAQAALAAYPV
                     QVVQGLAPAGMPPASDGVR"
     gene            complement(7086..8105)
                     /locus_tag="PLK64_04645"
     CDS             complement(7086..8105)
                     /locus_tag="PLK64_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HPV79063.1"
                     /translation="MSVQPSTAAKTERLLNLVIALLSTRRPLTKAQLRTAVPHYQQTA
                     SDEAFDRMFERDKDELRDLGIPLATQLVAAAYDDEVGYRIDRREYALPEIAFEPDEVA
                     ALALAGRAWNQASLASSAAAALTKLASAGVELDERSVVGIEPRVRTAEAAFASVHAAV
                     KARREISFVYRKANGTADTRRLQPWGMTQWRGRWYVTGMDVDRQAERVFRLGRIEGKV
                     SMSARVAAYEIPTDHVPRVLVAASEPPVVDQPPAVLRIRRGAGNTLRRRARTVGEVDD
                     EWSQIDLDYRDPEGLAQEVAGFGPDVVVEAPDSVRDAVIRLLGAARERHTATPADANA
                     PGGMP"
     gene            complement(8121..8507)
                     /locus_tag="PLK64_04650"
     CDS             complement(8121..8507)
                     /locus_tag="PLK64_04650"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018761348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPV79064.1"
                     /translation="MSLDPNRSKPELDYPGDAVPTDLVIEDLIEGDGAAVEAGQTVTT
                     HYVGWAFSTGEEFDASWNRGEPLSFRAGIGQVIRGWDQGLLGMKVGGRRKLTIPSHLA
                     YGERGAGGVIKPGESLIFVVDLVAVR"
     gene            complement(8582..9655)
                     /locus_tag="PLK64_04655"
     CDS             complement(8582..9655)
                     /locus_tag="PLK64_04655"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPV79065.1"
                     /translation="MPQPYAAHRLRAQTLRPRPHLSVVGLAVTALVALTACSSSTGSS
                     TPTGTRAATATATATASAAPTGPLGSAADQAVLDTITVGTAASGIAPTITLPTTPVSV
                     SITSRKILTPGGGALSAIGGLVRANILLVKASDGTTLDSTYEGSPQSFALNPKNYLPG
                     LANGLAGLNLGSRILLAIPASEGFGTAGRPDLGISGTETLVMVVDIVDVANAIPQATG
                     TPVAPVTGQPTVTFDPTTGPTITIPAGAAAPTTLVNQNLIEGSGTKVTEGMFLQVHYT
                     GVLWKDGSVFDSSWPRAQYYTFQTGTNSVIPAWDKGLVGHTVGSRVLLVVPPADGYGP
                     DGNPPKIAGTDTLVFVVDILAAA"
     gene            complement(9750..11111)
                     /gene="pafA"
                     /locus_tag="PLK64_04660"
     CDS             complement(9750..11111)
                     /gene="pafA"
                     /locus_tag="PLK64_04660"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HPV79066.1"
                     /translation="MKRRIFGVETEFGVTCTHEGQRRLTPDEVSRYLFRRVVAWGRSS
                     NVFLGNGSRLYLDVGSHPEYATAECDSIREVVVHDKAGERILEGLAEEAQQRLADEGI
                     AGDIYVFKNNTDSAGNSYGCHENYLIGRTGEFSLVSDMLIPFLISRQIICGAGKLASG
                     SKRATYCVSQRADHIWEGVSSATTRSRPIINTRDEPHADAEKFRRLHVIVGDSNMSET
                     TTLLKVGSADLVLRMAETGVVMRDLALENPIRAIREMSHDMTGRRTVRLADGRSMSAL
                     DMQSEYFTRVRAFADREGLTDGAHARVLELWERTLHAVETNNLALVEREIDWVIKWRM
                     IERYAAKHQLSLDDPRLAQLDLAYHDVNRRRGLFYLLQRRGLVTREASDLEVFRAKTV
                     APQTTRARLRGEFVKAAQAKRRDFSVDWVHLKLNDQAQRTVLCKDPFASVDARVDRLI
                     EAM"
     gene            11260..>12865
                     /locus_tag="PLK64_04665"
     CDS             11260..>12865
                     /locus_tag="PLK64_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79067.1"
                     /translation="MSDGSAYANPSVSAGGDVGDVTKHALEILARAQAHADTVKAQAD
                     EVFSAAQTRARELTAEAERLQREASALHAAAVSDRDQAAAVLASAHDDATSLLRDAAA
                     QASLVADAAARSSQAEIEEARRDAEALRAAALSDAEQQRRAAQVDFDTAMARFAAHEA
                     ERSVALDERLSAVEVEEANRLSMANTSASALVAAATADAERRTAAAREEADFIQAAAE
                     ALRERTVAETAQLRRQAAAEAQSRTASARADSDAMLSAAQAHLDWAERTMREIIDGAN
                     AEAERVTMTTARSLADHSRRRRQQLQGVLARVSHTSRQRLDDAAAESSRNRAQAEAIL
                     EAAAADAKRTEAEAAALAARLVADAETAAQTTVERAEHRLAEAEHGARLIREGTAAEV
                     EKLHREAHADRRAAREELVRTLAEAREEADRLRTQAREAVDSARAEVHTLAARRDDIN
                     TQLGHLSNVIEALAVPERMGLERNGVLRPSVSSAPVSPASAAPAAPAAAAPVALAAVP
                     PAALEPTTPAPVPPVALEPTTPAPVPP"
BASE COUNT         2012 a         4433 c         4539 g         1881 t
ORIGIN      
        1 cttcccagcg gcccatgcgg cggtctcggc ctgcaacttg atccccttga ccggcttctt
       61 ggccagaccg atcagaccgc gggcgagcat ctcggccttg ccgccacgcg gctcggcgct
      121 catcgggacc tcgggcagca cctcggtggg gtggtcggag agttggtcga acagcatcca
      181 cagaacgctg ccgtcggcca gaccgtgggt catcttgacg acataggcgt agcggccgtc
      241 ctccagcccc tcgatgacgt agatgcgcca catcgggcgg tcgcgctcaa gcaactgggc
      301 catgatccgg tcgatggcat cgtggagttg ggcgtcggtg ccgggcttgg gcagccggat
      361 gccgatgacc tgctccttga tgtcgaccgg tccgtccacc cagtagcgcc cgtccatctc
      421 caggggcacg ctcttgaggc gccaccgcag gaccggaagt cgcgtcagcc gttcggcaac
      481 ccgctcgcgc aggaaggcca gttcgtcgcg cttctcggcc ggcttgtcga agaaggcgat
      541 accggcgacg tgcccggtgg ttcgcccggt gtccaacgaa aggaacatct cgtccaagcc
      601 agtgagttgc tgcatgtctc tcctcgtaag gggggatcgt tgaataattc agtcatcaag
      661 tccttgacag tatttcaggc gatcccccat cgtggcagtc cgtccgccat ggcggacatc
      721 actacgcacc gtggaatacc ccatgagaag cgcacctcag cagcgctttg gccgctccga
      781 tgactcgatc cggccggttg aacccggtca gtcgatccgg tcggcggcca ccaccccgcg
      841 gcggaccgcg tcaacggcct tgcgggcctt ggcactcaag gcggggtgcg gcgcgatgtc
      901 gctgatctgc cccagcaggt cgaccacctg cttgcagcgg cgcacgaagt caccggcggc
      961 caggtcagag ccccgcagca ccgtctccag gcgttgcccg ctggcccacc ggtgcaccat
     1021 ccagacgatc ccggcatcgg gtgcgccgct cggcggcagc ccgtggagcc gctcctgctc
     1081 ctccaacgtg gcccacagcg ccgtcatcgc ggtgagggcc tcccccacgt ccgtcgtcgg
     1141 cacgcgcggg gcggcggcga ggtcctcgcg gcgcggttcg tggatgagcg ccgacacgac
     1201 ggcggccagg ccggcggcgt cgaggcggtt ccacacgccc tcgcggatgc actgagcggc
     1261 caggaggtcg cgttcggcat agatctggcg caggtcctgc ccagcctcgg tgaccgtgag
     1321 cccgccctcg ccgagatacc ccagatcctc caagaggtca cagatccggt cgaaggtgcg
     1381 cgcgacggtg ttggtgcggc cgtcgacctg acgcgcgagc gcatcggtct cccggcgcag
     1441 ccgccaccac ttctcggccc gccgggagtg ctcctcccgg tcggggcagg cgtggcaggg
     1501 gtgctcccgc agggccgcgc gcagtcgggt gacctccgcg tcggactccc ggcgcgcgtc
     1561 gtcgtcacca ccccgggagg tatgccgcgg cggcggctcg tgtgccacgg cgatccgcag
     1621 ggtggcggcc aggtcgcgtc gcgatttggg gctcttggcg ttgaacccgg tcgggatgcg
     1681 caccgtgagc aacggttgca ccggcgtcgg gacgtcgacg gtggtcagcc gacgcagttg
     1741 gcggtcttcg gtgacgaccg ccggtgaggc cgggcgtccc ggtgcgccac ggttggcggc
     1801 gacgaccacg gcatacccgg cgcgtcggcc ggtcggcacc cggatcacgt cgccgggacg
     1861 caaccgttcc agggacagtt cggcctcggc ccgcgtcgcg gcggaccgag ccttcgccgc
     1921 gcccttctcg aggtcacgca gggaggcgcg cagcgcggca tactccccga aatcgcccag
     1981 atgacagtgc cattcgacgg catactcggc caactgccgc tcgccgcggc gcaccgactg
     2041 cgccaggccg accacggaac ggtcggcctg gaactgcgcg aacgacgtct ccaacagctc
     2101 acgcgcacga tcgcgaccga cctgggcgac gaggttgacg gccatgttgt aggtggggcg
     2161 gaagctggag cgcagcgggt aggtgcgtgt cgaggcgagc ccgccgacga cgagcggatc
     2221 caggccacgg ctccacagga cgacgccgtg tccttcgacg tcgatgccgc gccggccggc
     2281 acgaccggtg agttgggtgt actccgcggg ggtcagctcg gcgtgggtgt cgccgttgta
     2341 cttgacgagt ttctccagga cgaccgtgcg cgcgggcatg ttgatcccga gggccagggt
     2401 ctcggtggcg aacaccgccc ggacccggcc ctgggtgaac aactcctcga tgatctcgcg
     2461 gaaggtcggc aacatcccgg cgtggtgggc cgcgaagccc cgttcgatgc cgtccacgaa
     2521 gtcccagtag cccaggacgc tcaggtcctc gtcggccagg gcgccgatcc gctcctcgac
     2581 ataccgccgg ttgcggacac cgtccgcctc gctgacgagc ctgatccccc gcgccagcag
     2641 ttgcccgaca gcggcatcgc acccgttgcg gctgaacacg aacgtgatcg ccggcaacag
     2701 cccttcgcgg tcgagggtcg cgatgacgtc cgcgcgggtg ggtccacccc cgggtcgtat
     2761 gccgccctcc cgccctcgcg caggtgcgtc cgactcccag gacccccgcc cgcctcgtgg
     2821 cccgcgtccg ccgccgggcg gccctccgcc aggcccggtc cgcccgcgca gtccgcgatg
     2881 ggcggcggag tcggcggcca gcgctcgctc gaccgagcgg atccgctcca acaactcggg
     2941 gttgacctgg acccggcgtc ccacctgcgg tcggccggag gagtccaagg actcctcgac
     3001 gaacaggtcg aagagttgtt taccgaccat gacgtgctgc cacagcggca ccgggcggat
     3061 ctcagagacg acgacggcca cgtcgccgcg aacctccccc agccactcgc cgaactcttc
     3121 ggcgttggac accgtcgccg acagcgagac gacgaggacg tcctcgggca ggtggatgat
     3181 cacctcttcc cagacagcac cccggaagcg gtcggcgagg tagtggacct cgtccatgac
     3241 gacgtaaccg aggcccgcca aggtgggcga accggcatac atcatgttgc gcagcacctc
     3301 ggtcgtcatg acgacgacgg gagcctcgcc gttgacggag gagtccccgg tgagcagccc
     3361 gacgttggcg gcgccgtggc gccgacagag atcggcgtac ttctggttgg acagggcctt
     3421 gatcggcgtc gtgtagaagg ccttgcggcc ggtggccaga gcgaggtgca cggcgaactc
     3481 gccgacgacg gtcttgccgg caccggtcgg tgccgcgact aggaccccgc gaccggcctc
     3541 gagggcctcg caacccgcaa cctggaaggg atcgagaccg aagtcgagcc cggcagcgaa
     3601 ccgggtcagc tcactcacgg tgcgacgacg gcgagggccc caggaacgac ctcaaggtcg
     3661 accggcagcg gcaggaagtc ctctccgtcg gcctggctgt cgatgccgtc cgcctcgagg
     3721 tgcacccgcc gcccgcgcag gatctcgacc gccgggtggc tcacgtggcc gccggagaag
     3781 accctgggga agatcctgag gaactcgccc ctcccgatct tgtgcaggat catcacgtcg
     3841 aacagcccgt cggtcatcga ggcgtcgggg cagaccttca tcccaccgcc gaaggttccc
     3901 ccattggcca cggcgacgag catcgcttcc gtctcgatcc tctggtcgtc cacctgcacg
     3961 acatagggga tgccggtgaa ccgcggcagt tccagggcga cggcggcgag gtaacgcggt
     4021 gtgccgtgca gggcggtgag gctgcgaccg cgggtgttga cgagggtgtc gaacccgcca
     4081 ccgaggaccc cgagccacca gagctcgccg tctgcggtca cacagcggcc ggcgtcgatg
     4141 cgtcgcgtct ggccgtcgga gatgagccgc gcgatcgcgg ggatgtcctt gcgcggcata
     4201 ccgaggctgc gggcgttgtc gttgccggtg cctgccggga ggacgacgag ggggatgtcc
     4261 gtgcccgcgc aggcgttggc gccgaggttg gccgtgccgt cgccgccggc gacgaccaag
     4321 gcgtcgatgg ccccggaccg gacggcgtcg aaggtgcgca cccgcccctc cgccgccgac
     4381 tgcgcggcga tgtccaggac gatgtgaccg ctatcggcga gtatgccgcg cagctgcgcc
     4441 gcggccgtct cgcttcgtcc gcgaccagac acggggttgg cgtagagccc gatcctgcgc
     4501 aacggcccgg tgaagtgagt cacgcggcga ggctacaggt cggaggcctc gtcgtcagcg
     4561 acgcggagcc actctgcgga ggggtcagag cgggcccgtc gacggtccag aagcagcgac
     4621 accccgacgg ccgcgaaata gagcatgacg agcgggacgg cgaggatgac catggtccac
     4681 gggttggggt ccggggtcat gacggcggtg aacaggaagc acatgaagac cgccccgcgc
     4741 cacgctcgca gcatcagtcg accggggagg atgtgcgcga cgttgagacc gacgaggaag
     4801 accggcagca ggaagcccaa cccgaagacg aggatgaact tgaggacgaa gctgaggtag
     4861 agcgagacgt cgatgatgtt cgaccccgac gtcggcgtga agctgaggag ggctcccatg
     4921 gccctcggta acacggcata cgccaaccag cagccgccga ggaacaacgg caccgcggcc
     4981 ccgatgaagg ccacggtgat gcgcttctcc ttgcgcgtga ggccggggac gatgaacgcc
     5041 cagatctgcc acagccagac cggcgacgcg accaccaccc cgacgaacag cccgacggtg
     5101 atcatcaccg agaacgagtc ggtgggcgtg ctgaacgaga ctgtcgtcag cgcgcctgcg
     5161 gtcttggtcc gagcaacttc ggtcaccggg ctggtgagag cgtcgtagat cgccacccaa
     5221 cgggtccagc cgaacaccgc gccgacgacg atcgccagtg ccgagatgac cgtccgtcgg
     5281 cgcaactcac gcaagtgatc cccgagggac atgtgtcctt cggggtcacg gttgcggcgg
     5341 aaccggaagc gggccatggg ggtgcggctc ggcactcacg agagtgaccg cccgagggcg
     5401 gcgactcagc tgtgccgagt gtcctctcag accgaggggc ggtcgtcggg cgaacggggc
     5461 gggacgacgt ccccgggcac gacgtcgtcg gcggccgggc tgggccgacc ttccttgccg
     5521 tcgttcttca tctcgttgac ctcagactta aagatccgca tcgagcgccc cacgctgcgg
     5581 gccatgtcgg gcaggcgctt ccagccgaag agcaggacga gagcaagaac cacaatgagc
     5641 cagtggctgg gttcacggag aaacatgctg gtcaggcctt tcgatggtcg gcgtctatcg
     5701 agtctacgcg gcgtgcccac cctggtaggg atgcccgacg acgcgccgct cgctcccccc
     5761 gcaacgtttc tcggatgagg tcacgttcct gagccacgtc ggcgggtgcg cgaaagatcg
     5821 ccaactcgac ctggggtcgg tgatcggtca ccccatcgcg ggggtcgacc tcgcgttccg
     5881 tcgccgccgc gaagagggtc tcagcctcgg cgagggtgtc gcccaacatc tcggtccggc
     5941 cccacagcca ccgcagccgg cgccagagat accaggcggc cccagcgacc agcgcgagcc
     6001 agatcagcgt ccagacgagg ccgcgcatca gcgcacgcca tcggaggcgg gcggcatacc
     6061 cgccggtgcc agcccttgca cgacctgcac ggggtatgcc gcgagcgctg cctgcgcgcc
     6121 ggcggccacc tgctcggcca gcgactgcgg ggcgaggatc tgcacgtgcc cgcccagccg
     6181 ccaggccaac cggcggaccc aactggggtc agcggcgcgc aggaggatcc ggcggcgacc
     6241 gtcgccggca tcggtcacgg agtccacggg gtagtagtcg gcgacccacg cggccgcctg
     6301 ctcgacgagc aactcgacga gcacgtcgtc cggcgagggt tgataggtct cgccggcaag
     6361 gtcacgcagt tgggccgact ccggcggggt cccgtcgacg tcgaggacct gcagcgattg
     6421 cacccgatcc aggcggaaca gccgcacgtc ctcggcccgg tggcaccacc cctcgagata
     6481 ccaacgggca tccaggctgg tcacccgcat cgggtcgacc tcgcgctcgg tcacctcgtc
     6541 acgaccgaac gactgatagc tcagccgcac gcggcggcgc tcctgaacgg ctcgggaggc
     6601 gtcggcgagg atcgtctcgg cggcgccgtc gtcgagggtg acccgcaccc gcgcggcgac
     6661 accggccgcc tcaccggtcg cctgttcgag cttggtcagg gcccgcagga tcgcctctcc
     6721 gccgtccgcg ccgctggctc ccgtgactcc gggggtcgcg gcgagggcac gcagccccac
     6781 catgagcgtg agggcctcgt cgacggtgaa ccgcagcggg cgtgagatgg cgtcggcatt
     6841 gccgacatag atctgtccgt cctcccagtc ggcctcgatg aggtcgtcgg gcaggtggcc
     6901 gggggtgccg cagacgaaca gcagcgccag gtcggactcg agttgctcgc ggctcactcc
     6961 gaattgagcg gccgccacgt cgacatcgac gccttggcga gccagcagcc acggcaccat
     7021 ggtcaacagc cgggacagcc gcgcggtggc gctctcctgc acggggcggc ctgcgcgggt
     7081 ggcggtcacg gcatacctcc aggcgcgttg gcgtcagcgg gcgtcgcggt atgccgctcg
     7141 cgcgccgcac cgagcagacg gatcaccgcg tcgcgcacgc tgtcaggtgc ttcgacgacg
     7201 acatccgggc cgaacccggc aacttcctgg gccaggccct cggggtcgcg gtagtcgagg
     7261 tcgatctggg accactcgtc atccacctcg ccgaccgtgc gggcccggcg gcgcagcgtg
     7321 ttgccggcgc ctcggcggat gcgcaggacc gcgggaggct ggtcgacgac gggcggctcg
     7381 gacgcggcga cgaggacccg cgggacgtga tcggtcggga tctcgtatgc cgcgacccgc
     7441 gccgacatgc tcaccttgcc ctcgatccgg ccgaggcgga acacccgttc ggcctggcga
     7501 tcgacgtcca tcccggtgac gtaccagcgg ccacgccact gggtcatccc ccagggttgc
     7561 agtcgccgtg tgtcggccgt gccgttggcc ttgcgataga cgaaggagat ctcgcgtcgg
     7621 gccttgaccg ccgcgtgcac ggacgcgaag gccgcctcgg cggtgcggac ccggggctcg
     7681 atcccgacga cggaacgctc atccagctca accccggcag aggccagttt ggtgagcgcg
     7741 gccgccgcgg aagaggccag gctcgcctgg ttccaggccc ggccggccag ggcaagcgcg
     7801 gcgacttcgt cgggctcgaa cgcgatctca ggcaaggcgt actcgcgccg gtcgatgcgg
     7861 tagccgacct catcgtcgta tgccgccgcg acgagttggg tggccaaggg gatccccagg
     7921 tcgcgcaact cgtccttgtc ccgctcgaac atccggtcaa aggcctcatc cgacgcggtc
     7981 tgctggtagt gcgggaccgc cgtgcgcaac tgggccttgg tgagtggccg acgggtagag
     8041 agcagggcga tgaccaggtt gagcagtcgc tcggtcttgg ctgcggtgct cggctggacg
     8101 ctcaccctcc cgacgctacc tcagcggacg gcgacgaggt cgaccacgaa gatgagcgac
     8161 tcccccggct tgatgacgcc acctgcgcca cgctcgccgt aggcgaggtg cgaggggatg
     8221 gtcagcttgc gacgaccacc gaccttcatg ccgagcagcc cctggtccca tccacggatg
     8281 acctgaccga tgccggcgcg gaacgacagc ggctccccgc ggttccacga ggcgtcgaac
     8341 tcctcgccgg tcgagaacgc ccatccgacg tagtgggtgg tgaccgtctg gccggcctcg
     8401 acggctgcgc cgtcaccctc gatgaggtct tcaatgacca ggtcggtggg aacggcgtcc
     8461 ccggggtaat ccagctcggg cttgctgcgg ttggggtcga gagacatgtc ggtttctcct
     8521 gtcggcggag tggtcagggg gtgaggcggt cagtgagtga agcggtcagt gagtgacgcg
     8581 gtcaggctgc ggcgaggatg tcgacgacga agacgagcgt gtcggtgccg gcgatcttgg
     8641 gcgggtttcc gtccgggcca tacccatcgg ccggcgggac gaccagcagg acgcgcgagc
     8701 cgacggtgtg acccaccagg cccttgtccc acgcggggat caccgagttc gtgccggtct
     8761 ggaaggtgta gtactgagct cggggccacg aggagtcgaa aacgctgccg tccttccaca
     8821 gcactcccgt gtagtggacc tggaggaaca taccctcggt gaccttcgtg ccgctcccct
     8881 cgatgaggtt ctggttgacc agggtcgtcg gtgcagccgc accggccggg atggtgatgg
     8941 ttggccccgt ggttgggtcg aaggtcaccg ttggctggcc cgtgaccggc gcgacgggcg
     9001 tgccggtcgc ttgagggatg gcgttggcaa cgtcgacgat gtccaccacc atgacgaggg
     9061 tctcggttcc ggagatgccc aagtccgggc ggccagccgt cccgaagccc tcggaagctg
     9121 ggatggccaa caggatccgc gagccgaggt tcagcccggc caggccgttg gccagcccag
     9181 gaaggtagtt cttggggttc agcgcgaacg actgaggcga tccctcatag gtcgagtcga
     9241 gggtggtccc gtcggaagcc ttgaccagca ggatgttggc ccggacgagt cccccgatgg
     9301 cgctcaacgc ccccccgcca ggggtcagaa tcttgcggga agttatcgac acagagacgg
     9361 gggtcgtggg cagggtgatc gtcggagcta tccccgaggc ggccgtccca accgtgatgg
     9421 tgtcgagcac ggcctggtcg gcggcgctgc ccaagggccc ggtcggggct gcggaggcgg
     9481 tggcggtggc ggtggcggtc gccgctctcg tgccagtggg cgtgctggag cccgtcgatg
     9541 aactgcacgc ggtgagagcc accagggcgg tgacggcgag gccgacgacg ctcaggtgag
     9601 gacgcggccg cagcgtctgg gcgcggaggc ggtgggcggc atacggctgg ggcacgggga
     9661 ggacctcggg tcgtgatggg gacgggcaaa ccccgccact ctaaccgggt gccctgtggc
     9721 ggcggacggg cgactggccg cagggagccc tacatggcct cgatgagtcg gtcgacccgg
     9781 gcgtcgacgc tggcgaacgg gtccttgcac aggacggtgc gttgcgcctg atcgttgagc
     9841 ttgaggtgga cccagtcgac gctgaaatcg cggcgtttgg cctgggcggc cttgacaaac
     9901 tcgccccgca gccgcgcccg ggtggtttgc ggcgccacgg ttttggcgcg gaagacctcg
     9961 aggtcgctgg cctcacgcgt gaccaggccg cgacgctgga gcaggtagaa cagcccacgt
    10021 cgacggttga cgtcgtggta agccaggtcg agttgggcca ggcgtgggtc gtccagcgac
    10081 aactggtgct tggcggcata ccgctcgatc atgcgccact tgatgaccca gtcgatctcc
    10141 ctctccacca gggcgaggtt gttggtctcg acggcgtgca aggtgcgctc ccacaactcc
    10201 aacacccgcg cgtgggcgcc gtcggtcagc ccctcgcggt cggcgaaagc ccgcacccgg
    10261 gtgaagtact cggactgcat gtccagcgcc gacatcgagc ggccgtcggc caaccggacg
    10321 gtccggcgac cggtcatgtc gtgactcatc tcgcggatcg cccggatggg gttctccagc
    10381 gcgaggtcgc gcatgacgac acccgtctca gccatgcgca ggaccaggtc ggcggacccc
    10441 accttgagca gcgtcgtggt ctcggacatg ttcgagtcgc cgacgatgac gtgcaggcgc
    10501 cggaacttct cggcgtcggc gtggggctcg tcgcgggtgt tgatgatcgg gcgactgcgg
    10561 gtcgtcgccg acgagacacc ctcccagatg tggtcggccc gctggctcac gcagtaggtc
    10621 gctcgcttgc tgccgctggc caatttgcct gcgccacaga tgatctgacg gctgatcagg
    10681 aaggggatga gcatgtctga gacgagcgag aactctccgg tgcgcccgat gaggtagttc
    10741 tcgtggcagc cgtaggagtt gcccgccgag tcggtgttgt tcttgaagac gtagatatcg
    10801 ccggcgatcc cctcgtcggc caggcgctgc tgggcctcct cggccaggcc ctcgaggatc
    10861 cgttcgcccg ccttgtcgtg gacgaccacc tcgcggatcg agtcgcactc ggcggtggcg
    10921 tactccgggt ggctcccgac gtcgagatag agccgcgacc cgttgccaag gaacacgttg
    10981 ctgctccgtc cccacgcgac gacgcggcgg aacaggtaac gcgacacctc gtcgggggtc
    11041 agacgtcgct gtccttcgtg ggtgcaggtc accccgaact cggtctcgac tccgaagatc
    11101 cgccgcttca ttccctcacc ctacgggcag gtatgccgcg tcccgaggga ccttgggcgc
    11161 cagtaggccc acaggaccag acagttcgtc cgttgggccg agaattggcc gacacacgcg
    11221 ggtgtgggaa gtagcgccca tatctgagat catgtccaca tgtccgacgg ttcggcgtac
    11281 gccaacccgt cggtctccgc cgggggcgat gtcggagatg tgaccaagca cgcgttggag
    11341 atcctcgcgc gtgcccaagc ccacgccgat accgtcaagg cgcaggccga cgaggtcttt
    11401 tcggctgccc agactcgggc ccgcgagctc accgccgagg ccgagcgact ccaacgggag
    11461 gccagcgcac tgcacgctgc cgcggtctcc gaccgcgacc aggccgccgc agtgctggcg
    11521 tcggcccacg acgatgccac ctcgttgctg cgggacgcgg cagcccaggc atctctcgtt
    11581 gccgacgcgg ccgcgcgcag cagccaggcc gagatcgagg aggctcgtcg cgacgccgaa
    11641 gcgttgcggg ccgcagcctt gagcgacgcc gagcagcagc ggcgtgccgc tcaggtcgat
    11701 ttcgacaccg cgatggcccg attcgccgct catgaggccg agcgttcggt ggcgctggac
    11761 gagcggttgt ctgccgtcga ggttgaggaa gccaaccgcc tgtcgatggc taacacgagc
    11821 gcgtcggcgc tcgtcgctgc cgccaccgca gacgccgagc gacgcaccgc tgcagcgcgt
    11881 gaagaggccg acttcatcca ggccgccgcc gaggcactgc gcgagcgcac cgtcgccgag
    11941 acggcccagt tgcgccgcca ggccgcggcg gaggcgcagt ctcgcactgc gtcggcccgc
    12001 gcggactccg acgcgatgct cagcgccgcc caagcccacc ttgactgggc cgagcggacc
    12061 atgcgcgaga tcatcgacgg cgccaacgcc gaggccgagc gagtgacgat gacgacggcc
    12121 cggtccctgg ccgaccactc gcggcggcgg cgccagcaac tccaaggtgt tctggcccgc
    12181 gtctcccaca cctcgcggca gcgactggac gatgcagctg cggagtccag ccgcaaccgc
    12241 gcccaggccg aggcgatcct cgaagcggca gcagccgacg ccaagcgcac cgaggccgag
    12301 gctgccgcgc tggccgcccg gctcgtcgcc gacgcagaga ccgcggccca gacgacggtc
    12361 gagcgcgcag agcaccgcct ggccgaggcc gagcacggcg cccggctcat ccgtgagggg
    12421 accgctgccg aggtcgagaa gttgcaccgt gaggcgcacg ccgaccgacg ggccgcccgc
    12481 gaagaactcg tccgcaccct ggccgaggct cgtgaggagg ccgaccgact gcggacgcag
    12541 gcccgtgagg cggtggactc ggcccgggcc gaagtgcaca ccctggcggc ccgcagagac
    12601 gacatcaaca cccaactggg gcacctgtcg aacgtcatcg aggcgctcgc cgtccccgag
    12661 cggatgggtc tcgagcgcaa cggcgtgttg cgcccctccg tgtcgtctgc tccggtctcc
    12721 cctgcatcgg ctgctcccgc cgcacctgcg gcggctgctc ctgtcgccct tgccgcggtt
    12781 ccgccagctg cactcgagcc gaccactcct gccccggtcc cgccagttgc actcgagccg
    12841 accactcctg ccccggtccc gccag
//
LOCUS       DAOMZY010000053        12838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_4511_length_12838_cov_5.902057, whole genome shotgun sequence.
ACCESSION   DAOMZY010000053 DAOMZY010000000
VERSION     DAOMZY010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 12838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12838
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4511_length_12838_cov_5.902057"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PLK64_04670"
     CDS             complement(<1..439)
                     /locus_tag="PLK64_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79068.1"
                     /translation="MTSLREAVRGATHVLAEAGVASAPVDALVLAAHALGVEVSEARR
                     LLIVGGVEVPPEYAALVAERARRVPLQHLTGEAHFRGLTLRVGPGVFVPRPETETLVS
                     LALDAIDAVDAAGAEDAVGTDGAGDPVGPGNRATTVGAGGAGGA"
     gene            complement(447..1571)
                     /gene="prfA"
                     /locus_tag="PLK64_04675"
     CDS             complement(447..1571)
                     /gene="prfA"
                     /locus_tag="PLK64_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPV79069.1"
                     /translation="MSDHTTEAAHALLASAAPLVAEHAELERSMADPELHADQSALRR
                     TTKRYAALGPVVAAYRALAAARADLAAAHELAGEDPDFAAELPAIEAVSALAQSRLHT
                     LLLPRDPDDDRDAILEVKAGEGGDESALFAGDLLRMYLRYAERRGWKTEVLDETQSDL
                     GGVKEARIALRAKGTPDIGEAPWARLKFEGGVHRVQRVPVTESQGRIHTSAAGVLVMP
                     DIDEGDDEVEIAPADLRIDVYRSSGPGGQSVNTTDSAVRITHLPTGVVVSCQNEKSQL
                     QNKESALRVLKARLRQAAREAAEATASAARRSQVRTVDRSERIRTYNFPENRIADHRT
                     GFKAYNLDVVLDGELDAVIQSCMDADEVERMAGAGEVGRS"
     gene            complement(1702..1920)
                     /gene="rpmE"
                     /locus_tag="PLK64_04680"
     CDS             complement(1702..1920)
                     /gene="rpmE"
                     /locus_tag="PLK64_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPV79070.1"
                     /translation="MKKDIHPAYGETQVTCTCGNTFVTRSTAKNGVIHADVCAACHPF
                     YTGKQKILDTGGRVARFEARYGKKAATK"
     gene            2138..2782
                     /locus_tag="PLK64_04685"
     CDS             2138..2782
                     /locus_tag="PLK64_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HPV79071.1"
                     /translation="MTRWIDVAHDVFVLAGVLAFAMSGALQAVGRRMDVVGICSLGVV
                     TAVGGGILRDLLIGAVPPAALTDWTLVPVALVGSLIVFWSHGRWTVPRRPMLVLDALG
                     LGLFCVEGTVKALGFGLNPYASAVVGMLTGVGGGVIRDVLAGQIPSVFRHGSRLYVIP
                     ALAGGGITAALWSAGHFSLLALLAVAATVSAIRIASLRFRWRAASPRDLDLDVG"
     gene            2892..4040
                     /locus_tag="PLK64_04690"
     CDS             2892..4040
                     /locus_tag="PLK64_04690"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPV79072.1"
                     /translation="MTPDEARDIARLGGRAATGIAGLVEHTHHGITERVYRTVGRLTG
                     GASRPIHVTHDLVARHTFFWVQRGLEAGAWAAQVIAARAMGGERSSRSGLDGVRAQLA
                     LGIVNGVTGDRLAADASTLAIPMSLRHEGRNVAPTAEGLRAAYGMGHERIVVFVHGLV
                     ETEHAWRFRSRQRWGEAGVSYASQLAADTDWVPLMVRYNSGAPIPANADAFADLLGEV
                     VRCWPGPLREVVIVGHSMGGLVALRALAHGDTAWTRAVRAVITVGSPREGAPLERFAV
                     VAEEVAESSGHGRWFGGLVGMRSYGIRNLRHPIDHPPLPDWVQEYAVLATLTPDSWHP
                     AVPRVGDGLVPVPADPSEHTAVVTGLHHLDLLNHPSVYAQLRAWLDPE"
     gene            4511..5245
                     /locus_tag="PLK64_04695"
     CDS             4511..5245
                     /locus_tag="PLK64_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79073.1"
                     /translation="MISHHTAAKLWGGVVPDTSAVHVSIPPGTSFSRAGVIVHRPQTL
                     PAVGTRRGVRLTPPEETFLACAAYLALVELVVLGDSLVGAGATTAEALITAAAHRRGR
                     GTRLARSAAALVRRGVDSPPETRLRLLLCLAGLPEPVVDHRIYSETGQLLYRFDLSYP
                     QIKLAIEYDGRHHERDPYRGRDLLRREDLEADGWAFVVVVTEHLNGTPGDVLARVARV
                     MQRRGMPVPRLSERWRQHFSQRERPA"
     gene            complement(5430..>7335)
                     /gene="rho"
                     /locus_tag="PLK64_04700"
     CDS             complement(5430..>7335)
                     /gene="rho"
                     /locus_tag="PLK64_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPV79074.1"
                     /translation="SAASGNSASGGSSASGGSGASSAPAATASAEGGSSAPARSRSGS
                     RRVRTGAGAPQSGSTGGAQASLPIGDGASDASSAAPAEQGERADRPASSGSSEQGERA
                     PRERAERFTRNREGQSGDQQGQQDRQQDRQQGGQQDRQQGGQQDRQQGGQQDRQQDRQ
                     QDRQQGGQQDRQQDRQQGQQGGQYRQQGGQGGQQNQSRYDDDIDSRTGRRRNRQRSRD
                     RKRGRGTMGMPMGGQELTEADVQIAEDDVIVPVAGILDVSDNYAFVRTSGYLPGPGDV
                     YVPLGLVRRHGLRKGDAVTGAVKALREGEIPVGQTGNRAKFNPLVRLDTVNGMDPDQA
                     KSRVEFSKLTPLYPQERLRLETLPNIITTRVVDLVSPIGKGQRGLIVSPPKAGKTLIL
                     QAIANAITTNNPEVHLMVVLVDERPEEVTDMQRTVRGEVIASTFDRPADDHTTVAELA
                     IERAKRLVELGHDVVILLDGITRLGRAYNLAAPASGRILSGGVDSAALYPPKKFFGAA
                     RNIEDGGSLTILATALVETGSKMDEVIFEEFKGTGNWELKLSRQLANRRIYPAVDVNA
                     SGTRREDILMSTEELRIMWKLRRVLSALDETQGIELLLDRLKKTKSNLEFLVQVQRTS
                     SIKLDGEEDN"
     assembly_gap    7336..7435
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7436..7750)
                     /locus_tag="PLK64_04705"
     CDS             complement(<7436..7750)
                     /locus_tag="PLK64_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012244849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HPV79075.1"
                     /translation="MTDTIEAAADRKSTKAGLTGLKLAELKDVASSLGITGTAKMRKD
                     DLVAAISASRGGGSSRGSGRASAGSAATSAAAARSAEPAASSDSAASGNSASGGSSAS
                     GGS"
     gene            complement(7992..9005)
                     /locus_tag="PLK64_04710"
     CDS             complement(7992..9005)
                     /locus_tag="PLK64_04710"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HPV79076.1"
                     /translation="MRKVPVGTAIRVRTPASSANLGPGFDSVGLALGVWDQLDVTVVA
                     EPGLRIEVTGSGADGVPRDESHLVYRSMVRGLRELGVEVPSGLLVQTVNAVPHGRGMG
                     SSATAIVSGVAAAQGLSACAAAVAAAAIEGRLGAAGEAVTIDLEAVNSLASELEGHPD
                     NASATVFGGMTLSWSAQDDLPIAVGASEGEAGTRHTVTVRLPLHPDLTAVVFVPETTL
                     ATHTARALLPERVLLRDAARNSARAALLVEAATRSPELLVPATHDWLHQEARRPSYAA
                     SMDLVDRLRADGHAATISGAGPSVLVLTTTDRVDSVRSGADEIWTRLVPGIPAEGVTV
                     RLR"
     gene            complement(9014..10120)
                     /gene="thrC"
                     /locus_tag="PLK64_04715"
     CDS             complement(9014..10120)
                     /gene="thrC"
                     /locus_tag="PLK64_04715"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPV79077.1"
                     /translation="MAHQWRGVIREYADRLPMLAGAPVVTLLEGGTPLIPAAYLSERV
                     GAQVWLKFEGLNPTGSFKDRGMTTAISVAAARGAKVVICASTGNTSASAAAYATKAGM
                     ACAVLVPDGKIAMGKLSQAIAHGATLLQVAGNFDDCLTVARKLAEAYPVELVNSVNPA
                     RIEGQKTAAFEIVDALGDAPDIHCLPVGNAGNITAYWQGYQEYAAGTPGAPGEAGTGG
                     PATHLPRMWGFQAAGAAPIVLGHPVDEPETIATAIRIGNPASWAQAVAARDDSGGRID
                     AVTDEQILDAHRILSGKEGVFVEPGSAASVAGLLARSESGDVPAGARIVCTVTGHGLK
                     DPQWALKRADGTEVVPTQIAAEAYAAAVALGLES"
     gene            complement(10122..11486)
                     /locus_tag="PLK64_04720"
     CDS             complement(10122..11486)
                     /locus_tag="PLK64_04720"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HPV79078.1"
                     /translation="MVSEREDVQGAPVRVALIGCGVVGSAVARLLIEHADDLAARVGR
                     PLELVGIGVRRPGRNRGDGLDPALFTTDSADLVTRADVVIEVVGGIEPARTLILRAMA
                     HGASVVTANKALLAEDGPTLYAAAAEHGVDLYFEAAVAGGIPLLRPLRESLAGDSVRK
                     VMGIVNGTTNYVLDKMDTTGQGFADAVSQAQALGYAEADPTADVEGFDAAAKAAILAS
                     LAFHTRVAGKDVHREGITEVTAADIQAAKDMDCVVKLLAICERTDGEDGSPAGVSVRV
                     HPAMIPRRHPLAGVREAFNAVFVEAEAAGQLMFYGRGAGGEPTASAVLGDVVAVARHR
                     VLGGRGPGESAYADLPLLDMGQALTRYHISLDVADRPGVLAQVASAFADHEVSIETVR
                     QQQVVGGAAADAASGSGGSTARASLVVVTHVAPEAALSATVDALAALPVVRGVNSVMR
                     VEGV"
     gene            complement(11526..>12838)
                     /gene="lysA"
                     /locus_tag="PLK64_04725"
     CDS             complement(11526..>12838)
                     /gene="lysA"
                     /locus_tag="PLK64_04725"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPV79079.1"
                     /translation="SVVRSRDGVLSVGGVSVTDLATQFGTPAYIVDEADFRARAAEFV
                     QTYNEIFEDLCGGADVYYAGKAFLCTEIARWIRDDGLFLDVCSGGELAVAIRAGMPGS
                     RIGMHGNNKSLAELEAAVDYGVGRIIVDSFEEIDRLAAVAARLGRTASVLVRVTVGVE
                     AHTHEFIATAHEDQKFGFSLASGAAAEAVRRILAHAPQLRLLGLHSHIGSQIFDTSGY
                     EVSARRILRLHAEIAREHGIEMPELDLGGGFGIAYTSEHDPLPPKSLAAEMADIVDRE
                     CRANGVGIPRISIEPGRAIAGPSTFTLYQVGTVKQVRLDGGASRSYVSVDGGMSDNVR
                     TALYDADFSCTLAGRASNATPVLTRVVGKHCESGDIVVKDEFLPADIVAGDLLAVPGT
                     GAYCRALSSQYNHTPRPPVVAVRDGKARVIVRRETVEDLLALDV"
BASE COUNT         1772 a         4441 c         4579 g         1946 t
ORIGIN      
        1 cggccccgcc ggccccgccg gccccgacag tcgtagccct gttgcccggg ccaactgggt
       61 cgcccgcccc atcagtgccg acagcgtcct cagcccccgc tgcgtcgact gcgtcgatgg
      121 cgtccagcgc gagcgagacg agcgtctcgg tctccgggcg cgggacgaac acccccggcc
      181 caaccctgag ggtcagcccc cggaagtgcg cctctcccgt gaggtgctgt agcgggacgc
      241 gccgggcgcg ctcggcgacg agggcggcat actccggcgg gacctcgacc ccgccgacga
      301 tgagcagccg acgggcttcg ctcacctcga cgccgagggc atgcgcggcc aggaccagcg
      361 cgtcgaccgg ggccgacgcg acgcccgcct cggcgagcac gtgggtcgcc ccccggaccg
      421 cctcgcgcag cgaggtcatg cgccggtcac gagcggccga cctcgccggc gccggccatc
      481 cgctcgacct cgtcggcatc catgcacgac tggatgaccg catcgagttc gccgtcgagg
      541 acgacgtcga ggttgtaggc cttgaacccg gtgcggtgat cggcgatccg gttctcgggg
      601 aagttgtagg tgcggatccg ctcggaacgg tcgacggtgc ggacctggct gcggcgcgcc
      661 gccgacgccg tcgcctcggc ggcctcgcgg gcggcctgac gcaggcgggc cttgagcacc
      721 cgcagcgccg actccttgtt ctgcagctgg gacttctcgt tctggcacga cacgaccaca
      781 ccggtcggca ggtgggtgat ccgcaccgcg gagtcggtcg tgttgacgga ctgcccgcca
      841 gggccgctgg agcggtagac atcgatccgc aggtcggccg gcgcgatctc gacctcgtcg
      901 tcgccctcgt cgatgtcggg catgacgagc acgccggcgg ccgaggtgtg gatgcgcccc
      961 tggctctcgg tgacgggcac ccgctggacc cgatgcacgc cgccctcgaa cttcaaccgc
     1021 gcccacggcg cttcgccgat gtcgggagtt cccttggcgc gcaatgcgat tcgtgcctct
     1081 ttgacgccgc cgaggtcgga ctgcgtctcg tcgagcacct cggtcttcca accgcgacgc
     1141 tcggcgtagc gcaggtacat ccgcagcagg tcaccggcga acagggccga ctcatccccg
     1201 ccctcccccg ccttgacctc gaggatcgcg tcccggtcgt cgtcgggatc gcgcggcagc
     1261 aggagggtgt gcaggcggct ctgggccagc gccgagaccg cctcgatcgc cggtagctcc
     1321 gccgcgaagt ccgggtcctc accggcgagt tcgtgagccg cggccagatc cgcgcgcgcc
     1381 gccgcgagcg cccggtatgc cgcgacgacc gggccgagcg cggcatacct cttggtcgtg
     1441 cggcgcagcg cgctctggtc ggcgtgcaac tccgggtcgg ccatcgagcg ttccagctcg
     1501 gcgtgctcgg cgacgagcgg cgccgccgag gcgagcagtg cgtgcgccgc ctcagtggtg
     1561 tgatcgctca tcgcaggctc cgatccggtc agtcagtggc tcaggcggca cgcggacaca
     1621 ccgccgcagg aacacgaacg ccggccccgc ggcataccct ccgcgtggtg cgggggatgc
     1681 gccgggccgg cggacgggac gctacttggt ggcagccttc ttgccgtaac gcgcctcgaa
     1741 gcgcgccacg cggccaccgg tgtcgaggat cttctgcttg cccgtgtaga acgggtggca
     1801 ggccgcgcac acgtcggcgt ggatgacgcc gttcttggcg gtgctgcggg tgacgaacgt
     1861 gttgccgcag gtgcaggtca cctgggtctc cccgtaggcg gggtggatgt ccttcttcac
     1921 ggttatctcc ttgtcggtga tggcgccggg tcggctgggt cgtgccttgg tatgccgtga
     1981 accgggccga gggtcaagtg tgccagaccc cagacgggtg acctaacgcg gcgttgcgct
     2041 gcggcattcc cgcagcctgg cgagggacgc gtgagggacc cgtgagggac cgctgaggga
     2101 ggcgtgagga acccgcgagg gactaccgtg gggcgccgtg acccgatgga tcgacgtggc
     2161 ccacgacgtg ttcgtgcttg ctggcgtcct ggccttcgcg atgtccggcg cgctgcaggc
     2221 ggtcggtcgg cggatggacg tcgtcggcat ctgctcgctc ggcgtggtca ccgccgtcgg
     2281 cggcgggatc ctgcgcgacc tgctcatcgg cgcggtgcct ccggccgccc tcaccgactg
     2341 gaccctggtt ccggtggcgt tagtcggctc cctcatcgtc ttctggtcac acggtcggtg
     2401 gacggtgccg aggcggccga tgctcgtcct ggatgccctc gggctggggc tgttctgtgt
     2461 cgaggggacc gtcaaggcgc tgggtttcgg gctcaatccc tatgcctccg ccgtggtcgg
     2521 catgctcacc ggggtcggcg gtggcgtcat ccgggacgtg ttggccggac agatcccgtc
     2581 cgtgttccgc cacgggtccc ggctctatgt catcccggcc ttggccggcg gtgggatcac
     2641 ggccgcgctg tggagtgcgg ggcacttctc gttgctcgcc ctgctggccg ttgcggcgac
     2701 cgtgtcggcc atccggatcg cctcgctgcg cttccggtgg cgggctgcca gcccccggga
     2761 cctcgacctc gacgtggggt gacatccggg gggtgacaga cgtggggtga ccgtcggggg
     2821 tgccacaggt caccccctaa gttacgcgac cgtaggtaca gtgaagagat gctgacgtca
     2881 gcatcccggt catgaccccc gacgaggcgc gcgacatcgc ccggctcggc ggacgcgctg
     2941 ccacgggcat cgccggcctc gtggagcaca ctcaccacgg catcaccgaa cgggtctacc
     3001 gcacggtggg ccgcctgacc ggtggggcga gccggccgat ccacgtgacc cacgacctgg
     3061 tggcacggca taccttcttc tgggtgcagc gggggctgga agcgggcgcc tgggcagcgc
     3121 aggtcatcgc ggctcgcgcc atgggcgggg aacgcagcag ccgcagcggc ttggatggcg
     3181 tgagggcgca gctggccctg ggcatcgtca acggggtcac cggcgaccga ctggccgcag
     3241 acgcctcgac gttggcgatc cccatgagcc tgcgacacga gggccgcaat gtcgcgccga
     3301 ctgcggaggg gctgcgcgcg gcatacggga tgggccatga gcggatcgtc gtcttcgtcc
     3361 acggcctggt cgagaccgag cacgcctggc gattccgctc gcggcagcgg tggggcgagg
     3421 cgggcgtgag ctacgcctcg caactcgcgg cggacaccga ttgggtgccg ctcatggtgc
     3481 gctacaactc tggtgccccg atcccggcga atgccgacgc cttcgccgat ctgctgggcg
     3541 aggtggttcg gtgctggcca gggccgctgc gcgaggtggt gatcgtcggg cattcgatgg
     3601 gtggcctcgt ggcgttgcgg gccctcgcac acggcgacac cgcctggacc cgcgcggtgc
     3661 gtgccgtcat cacggtgggc agccctcgcg agggcgcgcc actggaacgc ttcgcggtcg
     3721 tcgcggagga ggtcgccgag agcagcggcc acggacggtg gttcggtggg ttggtcggga
     3781 tgcgcagcta cgggatccgt aacctccgcc acccgatcga ccacccgccc ctgccggact
     3841 gggtgcagga gtatgccgtc ctcgccacgc tcacgcccga ctcctggcac ccggccgtcc
     3901 cgcgggttgg tgacggtctg gtgccggtgc cagccgaccc gtccgagcac accgcggtcg
     3961 tgaccggcct gcaccacctg gacctgctca accacccgag tgtctatgcc cagttgcgag
     4021 cctggctcga tcctgagtga ggggccggtg gggcgggcca cttcagcggg tgcgcttcgg
     4081 gccgaaactc gggcaactcg acactcgcgg ggttgccacg cctcacccag gctgactcgg
     4141 tcgggcaact cgacactcgc ggcctttcac cggtcggccc ctctggccca gcctgacgcg
     4201 gcctggtggc accctgtgga taacctctgc agaacaaccg cccgaggcgg cacagtgccg
     4261 ctatgagttc ccatgccgaa tctggcccgg ggcaacgtgg tcctagccaa cctgcgcacg
     4321 agcaaccctg gtcaccacgg cccggccagc ccttccttcg ccgaggggcg cagcaggccg
     4381 gcatcagcga cgatcacttg gcaggacctg gattccgagc catcttccgc ggtgtccacg
     4441 tgactgccga cgtgccatca ccctcacgct gcgcgcccaa gccgcgctca ctgcggcgcc
     4501 agccggggcc atgatcagcc accacaccgc ggccaagctg tggggtggcg tcgttcctga
     4561 cacctcagcc gtccacgtga gcatcccgcc aggcacgagc tttagtcgag ccggggtcat
     4621 cgtgcatcgg ccacagaccc tcccggccgt tggtacgaga cgcggtgtcc gactcacccc
     4681 gcccgaggag accttcctgg cgtgcgcggc atacctcgcg ctggtcgagc tcgtcgtgct
     4741 cggtgacagc ctcgtgggcg cgggggccac cactgcggag gccttgatca cagccgctgc
     4801 tcacaggcgc gggcgcggga cccggctggc ccgctcggca gccgcgctcg tgcgtcgagg
     4861 cgtcgactcg cccccggaga cgcggctgcg gttgctgctg tgcctcgccg ggctgcccga
     4921 gccggtggtc gaccaccgca tctattccga gactgggcag ctgctttacc ggttcgacct
     4981 gagctatccg cagatcaaac tggccatcga gtacgacggt cgccatcacg agcgcgaccc
     5041 ctaccgagga cgtgacctgc tgcgacgcga ggacctcgag gccgacggct gggcatttgt
     5101 cgtcgtcgtc accgaacacc tgaacggcac accgggggac gtcctggccc gggtcgcgcg
     5161 agtgatgcaa cgacgaggta tgccggtgcc gcgcctgagc gagcgctggc gacagcactt
     5221 ctctcagcgg gagcgcccgg cctgacagtt gcgctggcga gttctgacgg ttgccgcccg
     5281 gctaactcga cactcgctga gctgcgcggg ctggatagcg cggaagggcc aggaaactca
     5341 acactcgctg agttgcgcgg ggctggatag cgcggaaggg ccaggccaac tcgacactcg
     5401 ccgagttggc ctggccgaca ccgatctgac tagttgtcct cttcgccgtc gagcttgatc
     5461 gacgaggtgc gctggacctg gacgaggaat tcgaggttgc tcttggtctt cttgagccgg
     5521 tcgagcagca actcaatgcc ttgggtctcg tcgagggcgg agagcacccg acgcagtttc
     5581 cacatgatcc gcaactcttc ggtcgacatg aggatgtcct cacgccgggt gcccgaggcg
     5641 ttgacgtcga cggccgggta gatccggcgg ttggccagct ggcgggagag cttgagctcc
     5701 cagttgccgg tgcccttgaa ctcctcaaag atgacctcgt ccatcttgga gccggtctcg
     5761 acgagggccg tggcgaggat ggtgagcgag ccgccgtcct cgatgttgcg cgccgcaccg
     5821 aagaacttct tcggcgggta gagcgcggct gagtcgacac caccggagag gatgcgccca
     5881 ctggcgggtg cagccaggtt gtaggcgcgg cccaagcggg tgatgccatc cagcaggatg
     5941 acgacatcgt ggcccaactc gaccaggcgc ttggcccgct cgatggccag ctcggcgacc
     6001 gtggtgtggt cgtcggccgg acggtcgaag gtcgaggcga tgacctcgcc acgaaccgtc
     6061 cgctgcatgt cggtgacttc ctcaggacgc tcatcgacga gcacgaccat gaggtggacc
     6121 tcagggttgt tggtcgtgat ggcgttggcg atcgcctgca ggataagcgt cttaccggcc
     6181 ttcggcgggc tgacgatcag accacgctga cccttgccga tcggcgacac gaggtcgacg
     6241 acccgggtgg tgatgatgtt gggcaacgtc tccagacgca gccgctcctg cgggtagagc
     6301 ggggtgagct tggagaactc gacccgcgac ttggcctggt caggatccat gccgttgacg
     6361 gtgtccagcc ggacgagcgg gttgaacttg gcccggttgc cggtctgacc gacggggatc
     6421 tcaccctcac gcagcgcctt gacggcaccg gtcacggcgt cgcccttgcg cagcccgtgc
     6481 cgacgcacca ggccgagcgg cacgtaaacg tcgccaggcc ccgggaggta gcccgatgtc
     6541 cggacgaacg cgtagttgtc cgacacgtcg aggatgccgg cgacgggcac gatgacgtcg
     6601 tcctcggcga tctgcacgtc cgcctcggtg agctcctgcc cacccatcgg catacccatc
     6661 gtcccgcggc cgcgcttgcg gtcgcggctg cgctggcgat tgcgccgacg gccggtgcgg
     6721 ctgtcaatgt catcgtcgta gcgcgactgg ttctgctggc cgccctggcc accctgctga
     6781 cggtactgac cgccctgctg gccttgctgc cgatcctgct gacggtcttg ctggccgccc
     6841 tgttggcggt cctgctgacg atcctgctga cggtcctgct ggccgccctg ctgacggtcc
     6901 tgctggccgc cctgctgacg gtcctgctgg ccgccctgtt ggcggtcctg ctggcgatct
     6961 tgctggccct gctgatcgcc ggactgcccc tcgcggttgc gggtgaaacg ctcggcgcgc
     7021 tcccgggggg cgcgctcgcc ctgctcggaa gacccggacg aggccggccg gtcggcccgc
     7081 tcgccctgct cggcaggcgc ggccgagctg gcgtcggacg caccgtcgcc gatcgggagg
     7141 ctggcctgag cgccgccggt cgaaccgctc tggggggcac cggcaccggt gcggacccgg
     7201 cgcgaaccgg aacgggaccg ggccggcgcg ctggagccgc cctcggccga cgcggtcgcc
     7261 gccggagcgc tggacgcgcc gctgccgccg gacgcgctgc tgccgccgga cgcgctgttg
     7321 ccactggctg cgctgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngctgc
     7441 cgccggacgc gctgctgccg ccggacgcgc tgttgccact ggctgcgctg tcactggagg
     7501 cggcgggctc ggcgctgcga gccgctgcgg ccgacgtggc ggccgaaccc gcgctcgcgc
     7561 gtcccgaacc gcgggaggag cccccgcctc ggctcgccga aatggcggcc acgaggtcgt
     7621 ccttgcgcat cttcgccgtg ccggtgatgc ccaggctcga tgccacgtcc ttgagctcgg
     7681 caagcttgag ccccgtgagg cctgctttgg tcgacttgcg gtcggccgcg gcctcaatgg
     7741 tgtctgtcac gaaggatcct tcccctcgtt cgtcggcatc ccctgacggg agccgggtgc
     7801 tgcggccagc atcagctgac cggtgagttg accccacggt ggacgcgcgc aaggcggacc
     7861 gaggctgtca aggcctgtcg cgtgggggtg gtgccgccgc gccgtcgcgg gccactcggc
     7921 cctccggtgc ggtgcacgct caatgtaaca cctcggccgt cgccctgccc gcgtcccgcc
     7981 gcagacgcgt gtcaacgcaa ccgaaccgtc acaccctcgg cggggatgcc cggcaccagg
     8041 cgggtccaga tctcgtcggc cccggaacgc acggaatcga cccgatcggt cgtcgtcagg
     8101 accaagacgc tcggcccggc gccggagatg gtcgccgcgt gtccgtcagc tcgcaaccgg
     8161 tcgacgaggt ccatcgacgc ggcatacgag gggcgtcgcg cctcctggtg cagccagtcg
     8221 tgggtcgcgg ggaccagcag ttcggggctg cgggtggccg cctcgacgag cagcgccgcg
     8281 cgagccgagt tgcgggccgc gtcgcgcagc agcacgcgct cgggcagcag cgcgcgggcc
     8341 gtgtgagtgg ccaaggtcgt ttccggcacg aagacgactg cggtcaggtc ggggtgcagg
     8401 ggcaaccgca cggtgacggt atgccgtgtg cccgcctccc cctccgacgc cccgaccgcg
     8461 atgggcaggt cgtcctgggc cgaccacgag agggtcatcc ccccgaagac ggtcgccgag
     8521 gcgttgtccg ggtgcccttc gagctcactg gcgagggagt tgaccgcctc gaggtcgatc
     8581 gtgacggctt cccctgccgc gcccaggcgc ccctcgatcg cagccgccgc caccgccgca
     8641 gcgcacgctg aaagtccttg cgccgcagcg acccccgaga cgatcgccgt tgccgacgaa
     8701 cccatcccac gtccgtgcgg caccgcgttg accgtctgca cgagaagtcc cgacggcacc
     8761 tcgacgccga gctcgcgcag gcctcggacc atcgagcggt agaccagatg ggactcgtcg
     8821 cggggcaccc cgtcggcgcc cgatccggtg acctcgatgc gcaaccccgg ctcggcgacc
     8881 acggtgacgt cgagttggtc ccagaccccg agagccaggc cgacggagtc gaaacccggc
     8941 ccgaggttgg cgctgctggc cggggtccgc acccggatcg ccgtgccgac ggggaccttg
     9001 cgcatgatga gggtcagctt tcgagaccga gggcgacggc cgcggcatac gcctcggcgg
     9061 cgatctgggt cgggacgacc tcggtgccgt cggcgcgctt gagagcccac tgcgggtcct
     9121 tgaggccgtg gccggtgacc gtgcagacga tgcgggcccc cgcgggcacg tcgccggact
     9181 ccgagcgggc cagcaggccc gcgaccgagg ccgccgagcc gggctccacg aacacgccct
     9241 ccttgcccga gaggatccgg tgggcgtcga ggatctgctc gtcggtgacc gcgtcgatcc
     9301 ggccgccgga gtcgtcgcgg gccgcgacgg cctgggccca cgaggccggg ttgccgatcc
     9361 ggatggcggt ggcgatggtc tcgggctcgt cgaccgggtg gccgaggacg atcggcgccg
     9421 caccggcggc ctggaacccc cacattcggg gcaggtgggt ggccgggcca cctgtgcccg
     9481 cctcgccggg agcgccgggg gtgcccgcgg catactcctg gtaaccctgc caataggcgg
     9541 tgatgttgcc cgcgttgccc accggcaagc agtggatgtc gggggcgtcg cccagggcgt
     9601 cgacgatctc gaaggccgcg gtcttctgcc cctcgatgcg ggcggggttg accgagttga
     9661 ccaactcgac cgggtaggcc tcggcgagtt tgcgagccac ggtgagacag tcgtcgaagt
     9721 tgcccgccac ctgcagcaac gtggccccgt gggcgatcgc ctggctgagc ttgcccatgg
     9781 cgatcttgcc gtcgggcacc aggacggcgc aggccatccc ggctttggtg gcataggcgg
     9841 ccgcgcttgc gctcgtgttg ccggtggagg cgcagatgac gaccttggca ccgcgcgcgg
     9901 cggccaccga gatggccgtc gtcatcccgc ggtccttgaa cgacccggtc gggttgaggc
     9961 cctcaaactt cagccacacc tgcgcgccga cgcgctcgga gaggtatgcc gcggggatga
    10021 gtggggtgcc gccctcgagg agggtgacga ccggggcacc ggcgagcatc ggcaaccgat
    10081 cggcatactc gcggatgacc ccgcgccact ggtgggccat gtcagacacc ttccacacgc
    10141 ataacggaat tgacgcctcg aacgacgggc aacgcggcga gcgcgtcgac ggtggccgag
    10201 agggccgcct cgggggcgac gtgggtgacg acgacgaggc ttgcccgcgc ggtcgacccg
    10261 cccgagccgc tcgcggcatc ggcggcagcg ccgccaacga cctgctgctg gcgaaccgtc
    10321 tcgatcgaca cctcgtggtc ggcgaaagcc gaggccacct gggcgaggac gccaggacga
    10381 tcggcgacgt cgaggctgat gtggtagcgg gtgagggcct ggcccatgtc gagcagaggc
    10441 aggtcggcat aggcgctctc ccccggcccg cggccaccga ggacccggtg ccgagcgaca
    10501 gcgacgacat cgccgaggac ggcgctggcc gtcggctcgc caccggcacc gcgcccgtag
    10561 aacatcagtt ggccggccgc ctcggcttcg acgaagacgg cgttgaaagc ctcacgcacg
    10621 ccggcgagcg ggtgccgccg aggaatcatc gccgggtgga ctcgcacgga aacccctgca
    10681 ggagagccgt cttcgccatc ggtgcgctcg cagatcgcga gcagcttgac gacgcagtcc
    10741 atgtccttgg cggcctggat gtcggccgcg gtgacctcgg tgatgccctc gcggtggacg
    10801 tccttgccgg cgacccgagt gtggaaggcg aggctggcga ggatcgccgc cttggccgcc
    10861 gcgtcgaagc cctcgacatc ggccgtcggg tcggcctcgg cataaccgag cgcctgggcc
    10921 tggctcaccg cgtcggcgaa gccctgcccg gtcgtgtcca tcttgtcgag cacgtagttg
    10981 gtcgtgccgt tgacgatccc catgaccttg cgcacggagt cgccggcgag tgattcgcgc
    11041 agcggccgca gcagaggtat gccgcccgcc accgccgcct cgaagtagag gtcgacgcca
    11101 tgctcggcgg cggcggcata cagggtcggg ccgtcctcgg cgaggagagc cttgttggcc
    11161 gtgacgaccg aggcaccgtg cgccatggcg cgcaggatga gggtgcgggc gggctcgatg
    11221 ccgccgacga cctcgatgac gacgtcggcg cgggtgacga ggtcggcgga gtcggtggtg
    11281 aagagggccg ggtccagccc atcacccctg ttgcgccccg gccggcggac gccgatgccg
    11341 acgagttcca gagggcgacc cacccgggcg gccagatcgt cggcgtgctc gatgagcagc
    11401 cgggccaccg ccgagccgac cactccacag ccgatgaggg cgacccgcac cggggcgccc
    11461 tgcacgtctt cgcgttcact caccattgcg tttactcacc acgaggcacc atgatcccgc
    11521 atccctcaga cgtccaacgc caggaggtcc tcgacggtct cgcggcggac gatgacccgc
    11581 gccttgccgt cccggacggc gacaaccggc ggcctcggcg tgtggttgta ctggctggac
    11641 agggcgcggc aataggcgcc ggtacccggc actgcgagca ggtcgcccgc cacgatgtcg
    11701 gcggggagga actcgtcttt gacgacgatg tcaccactct cacagtgctt tccgacgacg
    11761 cgggtgagca ccggcgtcgc gttggacgcg cgtccggcca gcgtgcagga gaagtcggcg
    11821 tcgtagagcg cggtgcggac gttgtcgctc atgcccccgt cgacggagac gtaactgcgg
    11881 ctggcacctc cgtcgaggcg gacctgcttg accgtgccga cctggtagag cgtgaaggtg
    11941 ctcggcccgg cgatcgcccg gccaggctcg atcgagatgc gcgggatgcc gaccccgttg
    12001 gcccgacact cccggtcgac gatgtcggcc atctccgcag ccaaggactt gggcggcagc
    12061 gggtcgtgct cgctggtgta ggcgatgccg aacccaccac cgaggtcgag ctcgggcatc
    12121 tcgatgccgt gctcgcgggc gatctcggcg tgcaggcgca ggatccggcg ggccgagacc
    12181 tcatagccgg aggtgtcgaa gatctgcgag ccgatgtggc tgtgcaggcc gagcagccgc
    12241 agctgggggg cgtgggcgag gatccgccgg accgcctcgg ccgcagcacc cgaggcgagg
    12301 gagaacccga acttctggtc ctcgtgggcg gtggcgatga actcgtgggt gtgagcctcg
    12361 acgccgaccg tgacccggac gaggaccgac gccgtgcgac caagccgagc ggcgacggcg
    12421 gccaaccgat caatctcctc gaaggagtcg acgatgatcc gcccgacgcc gtagtcgacg
    12481 gccgcttcga gctcggccag gctcttgttg ttgccgtgca tgccgattcg cgacccgggc
    12541 atccccgccc ggatggcgac cgccaactcg ccgccgctgc acacgtcgag gaacaggccg
    12601 tcgtcacgga tccagcgggc gatctcggtg cacaggaacg ccttgcccgc gtagtagacg
    12661 tcggctccgc cgcacaggtc ctcgaagatc tcgttgtagg tctgcacgaa ctccgccgcg
    12721 cgggcccgga agtctgcctc gtcaacgatg taggccggcg tcccgaactg cgttgccagg
    12781 tcggtcaccg acacgccccc aacagagagg acaccgtcgc gggagcgcac cacgctgg
//
LOCUS       DAOMZY010000054        12727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_4568_length_12727_cov_5.902304, whole genome shotgun sequence.
ACCESSION   DAOMZY010000054 DAOMZY010000000
VERSION     DAOMZY010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 12727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12727
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4568_length_12727_cov_5.902304"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PLK64_04730"
     CDS             complement(<1..696)
                     /locus_tag="PLK64_04730"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HPV79080.1"
                     /translation="MTVTVLHLTDREHDDFVRDHRHGHLMQTVAWGKVKEPTGWTWQR
                     VAVGRHTPEGPEVTGAALLLFRRTPVLPLALCYCPRGFVVDWADTESVNALLHEVTRV
                     AKANSAVTLRIDPAVPIDNDFVAPALRSRGFTRTDPGDDAAKALAQPAMRMVTGIGDL
                     DGLLKELPQGTRWSIRKAEKSGLVYESAGREGIDAFHDLLRTTGERDDFGVRSKGYFT
                     GLYDLLSPTDNITI"
     gene            complement(760..1542)
                     /locus_tag="PLK64_04735"
     CDS             complement(760..1542)
                     /locus_tag="PLK64_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HPV79081.1"
                     /translation="MTKSDGPRVTDPAHRDGMRAFEVVLAWVESRILAGELCVGDQLP
                     PERELAKQLEVSRAAVREAVRTLQAQGVVRSAVGAGGTGGTTVTAVPAHALTRLLRLH
                     VALANFAVDDVIEARIAMERLSIRLACRNARASDLAEMRRAIAVMDDPEIDKSRFNDA
                     DTALHLAIAEAAGNRLAADFTGAIRESIRGPLLDAIRDVAGWEDLIETLRADHRRLYA
                     AIERRDADAAESLVEDHIRSAWRQLRPGVPAPMVASEVRARR"
     gene            1731..3509
                     /locus_tag="PLK64_04740"
     CDS             1731..3509
                     /locus_tag="PLK64_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HPV79082.1"
                     /translation="MIPALAITTDAYQPDLAPLGGSLVGSALIALIPLLTVFVTLGWL
                     RWKAHVAGLTAVAVAILVAVLVYRMPPGLAVLSATEGAVFGLFPIMWIVFTAIVLYQI
                     TVVSGRFEDLRATFHLISDDPRIQAIIIAFCFGGLLEALAGFGAPVAITGVMLMALGF
                     SPLRAAAVVLLANTAPVAFGAIGTPIITAGALTKIPYTEIGAYVGHQTPFIAAFVPLL
                     LVLMVDGRRGVRQTWPVAVVVGVAFAVAQWISATYISVELTDIIASLVGLAVAVVFLR
                     FWQPVGKEAAHATLAVERERDLVLAAAGSVATATGKGGGPSGAKSATAAYAVSAQELE
                     TRARGLTKGRIVMALFPYLIVIAVFSIAKLVAPVKAWLASTDLKIPWPGLDGNVLTAT
                     GAKNTSTVYTLPWLSGPGTSLLICSLITAVVYGVTMAAWGKELKETAYKMRFAFLTVS
                     SVLALAYVMNLSGQTITVGTWIAGTGAAFAFFSPVLGWIGTAVTGSDTSANALFATLQ
                     QTAAKGAGLDPTLLVAANTSGGVVGKMISPQNLTIAATAVGLVGRESDIFRKVILWSV
                     GLIIVMCLLVGLQSTVLAWMLPEFVK"
     gene            3506..4309
                     /locus_tag="PLK64_04745"
     CDS             3506..4309
                     /locus_tag="PLK64_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPV79083.1"
                     /translation="MTASSITPTVTPAVTPTVAKGLKVALFAACYNDTMWPGTPIAVV
                     KLLERLGCRVEFPQRQTCCGQIFTNTGYAAEALPLVRGFVDTFAAYDAIVAPTGSCVG
                     AIRHQHHDLAAAAGDVALTRAVDALTPKVYDLSEFLIDVLGVTDVGAYFPHRVTFHPT
                     CHSIRVAGVGDRPQRLLQAVRGLTLVDLPHKEECCGFGGTFSLKNADVSIAMASDKAR
                     HVRETGAEVLVAGDNMCLMNMGSVLARQRAGVRVMHLAEVLASTEEDPA"
     gene            4387..5820
                     /locus_tag="PLK64_04750"
     CDS             4387..5820
                     /locus_tag="PLK64_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HPV79084.1"
                     /translation="MPKFPKAAKEALANTQQRRNLAHATGVIRAKRAGVVAELPQWED
                     LRLAGAAAKDETMTHLAAYLEQLEANLVRNGAQVHWARDAAEACQIVVDIAAAKGVDE
                     VVKVKSMATQEIELNEALEAAGIHAWETDLAELIVQLGHDRPSHILVPAIHRNRSEIR
                     EIFRREMHLVGRPAPEDLTDEPRRLAEAARLHLREKFLRAKVAVSGANFAIADTGTLV
                     VVESEGNGRMCLTLPETLISVVGIEKVLPTWQDLGTFMQLLPRSSTGERMNPYTSMWT
                     GVHPGDGPQEVHVVLIDNGRSHVLADPLGRQALRCIRCSACLNVCPVYERAGGHAYGS
                     VYPGPIGAVLNPQLRGTASDVDKSLPFASTLCGACFEACPVRIDIPSLLVHLRAQVVD
                     ESGRKSAEAKAMKAMAWMMGDYRRLEKAQKAATLGGRLLKGRTIHTMPGPFAPWTSAR
                     DVPSPPTSSFRDWWKARNTDSTTEDQR"
     gene            5817..6554
                     /locus_tag="PLK64_04755"
     CDS             5817..6554
                     /locus_tag="PLK64_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HPV79085.1"
                     /translation="MTTTSLPAADSAARTAEQLRTAEQIRNDAARAAIFGRVAAAVTD
                     ISAPLGARGDAPRIEQPSPGRAQTVELFIENVVDYRATVVPIAPGKVGSAIAKALKAA
                     TSVVVPSGLDPAWTAGLPDGIRIVSDDHLDNAALDGVAAVVTAATVGVATTGTIVLDH
                     GPDQGRRALSLVPDLHVCVVRASQVVHDVPEAVARLRSTVDQGASAGMPRPLTWISGP
                     SATSDIELERVEGVHGPRTLHVILVQD"
     assembly_gap    6708..6807
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6843..8033)
                     /locus_tag="PLK64_04760"
     CDS             complement(6843..8033)
                     /locus_tag="PLK64_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPV79086.1"
                     /translation="MADRTPQTPLGLFGTDALIDAEDRAIRDTVRAVLTAKVRPHIGE
                     WFESGTIPARELAVELGALGLLGMHLEGYGCAGTSATAYGLACVELEAVDSGVRSLVS
                     VQGSLAMFALYRFGSEEQKQEWLPRMATGEAIGCFGLTEPDFGSNPAGMRTYAARDGS
                     GDDADWVLTGTKMWITNGSVADVAVVWAQTDEGIRGFIVPTASPGFSAPAIKGKLSLR
                     ASVTSELVLDQVRLPASAMLPLAKGLSGPLSCLTEARFGIIFGALGAARDCLETTLEY
                     VATRDIFDKPLAGYQITQTKLADMTLELGKGMLLALHLGKLKDAGTLRPEQVSLGKLN
                     NVRESIAIARECRTLLGANGITLDYPVLRHANNLESVLTYEGTSEVHQLVIGQALTGQ
                     AAFR"
     gene            complement(8112..8855)
                     /locus_tag="PLK64_04765"
     CDS             complement(8112..8855)
                     /locus_tag="PLK64_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPV79087.1"
                     /translation="MRLQDKVTIVTGAGAGIGRAIARVTAAQGAIVVVTDLDGPAAEA
                     VATEIGGSASWYAVDVTDRASVAAMVAAVTAAHGRIDVLVNNAGWDKAVPFVDSEPDL
                     WRKVIDINYVGVLNTCREVLPVMAAQNRGRIVNLGSDAGRVGSSGEAVYSGAKGAIIA
                     FSKTIARESARHQVTVNVVCPGPTDTALFASMGGDNPKLREGLTRAIPLRRLAAPEDI
                     ANAVAFFASDEAAYITGQTLSVSGGLTMS"
     gene            complement(8883..10028)
                     /locus_tag="PLK64_04770"
     CDS             complement(8883..10028)
                     /locus_tag="PLK64_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPV79088.1"
                     /translation="MDYGLDSEQEDFRQAVRQFATKALAPHYQRDDKTATFRREQALD
                     LAAMGLTGLRIPEAYGGQDASAVIAGIATEEVGRADFNATYVIINTALVSDIIVRNAT
                     PEQQAAWLPGIAAGTTVPCICITEPGHGTDAANLELKAIRTATGWTLHGEKTSITLGM
                     ASDRAVVLARTGGPGARGVSAFWVDLHDPTVTRSPFDDLGSRAIGRAALHFDGTPVGD
                     DDLIGAEGGGFVSVMQGFDYSRAIIGLGCIGAAEASLDEALQWSRDRIAFGQPIGTFQ
                     GVAFPLAECATYLKGARHICLEALWRKDTGLDHSAEAAMAKFWAPKLATEVIHQCLLT
                     VGHVAYSSDCPIGQRLRDVIGLEIGDGTAQVSKLVISRHLLGRDFAP"
     gene            complement(10038..10835)
                     /locus_tag="PLK64_04775"
     CDS             complement(10038..10835)
                     /locus_tag="PLK64_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPV79089.1"
                     /translation="MTPFEDITYVVQDSAAIVTINRPERYNAFRAQTVEELIKAFRLA
                     WADRQVQSVILTGAGDKAFCTGGDVKQRAETGDYGPSESGMFEIGALHKVIRDIPKPV
                     IAAVNGVAVGGGHVLHVLCDVSIASETARFGQAGPRVGSFDAGFGTAYLARVVGEKKA
                     REIWFWCRMYSAQEALAMNLVNKVVPPGEVMAEAKAWADEVATKSPTAIRFLKQSFNA
                     DTDHHAGLSNLAMSALDLFTASPEGLEGAAAFAEKRTPDFNHHVNWH"
     gene            10909..11526
                     /locus_tag="PLK64_04780"
     CDS             10909..11526
                     /locus_tag="PLK64_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPV79090.1"
                     /translation="MSSADPSREARAEARVETRRRQILQSATRIMIRTGYHEMSMQAV
                     ADDAGMSVGLIYQYFGGKQEVLRAVIVDILDTFRAEVPAAMAAAGADPEAQLAAGFAS
                     FCRVIDAKRAGTMLAYRESQTLDAAGQAEIIRLEQETIDPFRVAVEAGIAAGVFRPVP
                     AELVAHNLKLAAHGWALKHWDVGARLDLTAYIDAELDLLLAAIRA"
     gene            complement(11558..>12727)
                     /locus_tag="PLK64_04785"
     CDS             complement(11558..>12727)
                     /locus_tag="PLK64_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV79091.1"
                     /translation="PDGYLRERATYAVMNGVVTETVDGARTVDALGLSARRRRRMDEA
                     LRASFDAEMYTLRLRLLWFPQVEFAYLLPVAATLVWGGWLVSQGHASIGAVTTVALYV
                     HQLANPLDDLLMWLDEIQVGATSLARVIGIADVPPDREATGETPVDAHLVVDDVSYAY
                     RAGRDVLRGVSLDLAPGERLAIVGPSGAGKSTLGRLMAGIDGPRLGRVDVGGVRLVDL
                     DLDRLRGEVALVTQEHHVFVGTLADNLLLARPSASRDELLDALTAVDGREWALGLADG
                     LDTRVGSGAHSLTTAQSQQLALARLVLADPHTLVLDEATSLLDPRAARHLERSLAAVV
                     TGRTVVAIAHRLHTAHDADRVAVVDGGLVTEIGSHDELIAAGGAYAALWRSWRDE"
BASE COUNT         1918 a         4386 c         4340 g         1983 t
ORIGIN      
        1 gatggtgatg ttgtcggtgg gggacagcag gtcgtagagc ccggtgaagt agcccttgct
       61 gcggaccccg aaatcgtcgc gctcgccggt cgtgcgcagc aggtcgtgga aggcgtcgat
      121 gccctcgcgt ccggccgact catagaccag gccagacttc tctgccttgc ggatgctcca
      181 gcgggtgccc tgggggagct ccttgagcaa cccgtcgagg tcgccgatcc cggtcaccat
      241 ccgcatggcg ggctgggcga gggccttcgc cgcgtcgtcg cccgggtcgg tgcgcgtgaa
      301 gccgcgcgag cgcagcgccg gcgcaacgaa gtcgttgtcg atcggaaccg ccgggtcgat
      361 ccgcagggtc accgccgagt tggccttggc cacccgggtc acctcgtgca ggagcgcgtt
      421 gacgctctcg gtgtcggccc agtccaccac gaagccgcgc gggcagtagc acagggccaa
      481 cggcaggacc ggggtgcgcc ggaagagcag cagcgccgct cccgtgacct cgggtccctc
      541 aggggtatgc cgtcccaccg ccacccgctg ccaggtccag cccgtgggtt ccttgacctt
      601 gccccacgcc acggtctgca tgagatggcc gtggcggtgg tcgcggacga agtcgtcatg
      661 ctcgcggtcg gtgaggtgca gcaccgtcac agtcacgagg cactccttcg ccctggtgcg
      721 atccgatcgc agggcaccgt ccaggtcatc cgggagagtc taccgtcgcg ctcgcacctc
      781 actcgcgacc atgggcgccg ggacgccggg gcgcaactgt cgccaggcag agcggatgtg
      841 gtcctcgacc agcgactcag cggcatcggc gtcgcggcgt tcgatcgcgg catacaggcg
      901 ccggtggtcg gcgcgcaggg tctcgatgag gtcctcccag ccggcgacgt cgcgaatcgc
      961 gtcgagcaag gggcctcgga tggactcccg aatggccccc gtgaagtcgg cggccaaccg
     1021 gttgccggca gcctcggcga tcgccaggtg cagggccgtg tcggcgtcgt tgaagcgcga
     1081 cttgtcgatc tccggatcgt ccatgacggc gatggctcgg cgcatctcgg ccaggtcgga
     1141 ggcccgggcg ttgcggcagg ccaagcgaat actcaaccgc tccatagcga tccgagcctc
     1201 gatgacgtcg tcgacggcga agttcgccag cgccacgtgc aaccgcaaca gccgggtgag
     1261 ggcgtgggca gggaccgcgg tgacggtggt ccctccggtg ccgccggcac ctaccgcgga
     1321 gcggacgacg ccttgggcct gcaaggtccg cacggcctca cggaccgcgg cccggctcac
     1381 ctcaagttgc ttggccaact cacgctccgg gggaagttgg tcaccgacac acaactcacc
     1441 ggcgaggatc cggctctcga cccaggcgag gaccacctcg aacgcccgca tcccgtcgcg
     1501 gtgcgccggg tcggtgaccc gaggtccgtc ggacttggtc atacccgtgc ctcctgcctg
     1561 cgagcacccg tcccgcgact gggcccttga cgtcctaatg gtcagacctt accctaggga
     1621 caaccctaat tggtcagacc accctccagc gtgactcgag aatggtcaga ccacttggtc
     1681 gaccgagccg gtgtcgtccc gatcgaaccc agtcagagga gatcgcccgc atgatccccg
     1741 ccctcgccat caccacggat gcctatcaac ccgacctcgc accgctgggg ggaagcctcg
     1801 tcggctccgc cctcatcgcc ctcattccgt tgctgaccgt cttcgtcacc ctggggtggc
     1861 tgcggtggaa ggcgcacgtg gccgggctca ccgccgtggc cgtcgccatc ctcgtggccg
     1921 ttctcgtcta tcggatgccg ccgggcctcg ccgtgctctc ggccaccgaa ggcgccgtct
     1981 tcggcctctt ccccatcatg tggatcgtct tcaccgcgat cgtcctctac cagatcaccg
     2041 tggtcagcgg ccggttcgag gacctccggg ccaccttcca cctcatctcc gacgacccac
     2101 ggatccaagc gatcatcatc gccttctgct tcggcggcct ccttgaggcc ctcgctggct
     2161 tcggcgcccc ggtcgccatc accggcgtca tgctcatggc cctgggcttc agtccactgc
     2221 gcgccgcggc cgtcgtcctg ctcgccaaca ccgcacccgt ggccttcggc gccatcggca
     2281 ccccgatcat caccgcgggc gccctgacca agatccccta caccgagatc ggcgcctatg
     2341 tcggacacca gaccccgttc atcgccgcgt tcgtcccgct gctgctcgtc ctcatggtcg
     2401 acgggaggcg gggagtgcgc cagacctggc cggtcgccgt ggtcgtcggc gtcgccttcg
     2461 ccgtggctca gtggatcagc gccacctaca tctcagtcga actcaccgat atcatcgcct
     2521 cgctcgtcgg cctcgcggtc gccgtcgtgt tcttgcggtt ctggcagccg gtcggcaagg
     2581 aggccgccca cgcgaccctg gccgtggagc gggagcgcga cctcgtgctc gccgctgccg
     2641 gttcggtcgc caccgcgacc gggaagggcg gcggtcccag cggcgccaag agtgcgaccg
     2701 ctgcgtatgc cgtgtccgct caggagttgg agacccgcgc tcgcggcctg accaagggcc
     2761 ggatcgtcat ggcgctcttc ccctacctca tcgtcatcgc cgtcttctcg atcgccaagc
     2821 tcgtcgctcc ggtcaaggcc tggctggcca gcaccgacct caagatcccc tggccaggcc
     2881 tggacggcaa cgtcctcacg gccaccggag ccaagaacac ctcgacggtc tacacgctgc
     2941 cttggctctc gggccccggc acgagcctgc tgatctgttc gctcatcacc gccgtcgttt
     3001 atggcgtgac catggcggcc tggggcaagg aactcaagga gacggcatac aagatgcggt
     3061 ttgcgttcct cactgtgtcc tcggtcctcg ccctcgccta cgtgatgaac ctgtccggtc
     3121 agacgatcac cgtcggcacg tggatcgccg ggaccggggc cgctttcgcc ttcttctcgc
     3181 cagtcctcgg gtggatcggc accgcggtca cgggctcgga cacgagcgcc aacgcgctgt
     3241 tcgccaccct ccaacagacg gccgccaagg gtgccggcct cgacccgacc ctgcttgtcg
     3301 ccgccaatac ctcaggcggt gtcgtgggca agatgatcag cccacagaac ctcaccatcg
     3361 cagcaaccgc ggtcggcctg gtcggccgcg agtccgacat cttccgcaaa gtcatcctgt
     3421 ggagtgtcgg gctgatcatc gtcatgtgtc ttctcgtcgg cctgcaatca accgtgctgg
     3481 cctggatgct cccggagttc gtcaaatgac cgcgtcatca atcaccccca ccgtcacccc
     3541 ggccgtcact ccgacggtcg ccaagggtct caaggtcgcc ctcttcgccg cgtgttacaa
     3601 cgacacgatg tggccgggca ccccgatcgc cgtggtcaag ctccttgagc ggctggggtg
     3661 ccgggtggag ttcccacagc ggcagacctg ctgcggtcag atcttcacca acacggggta
     3721 tgccgccgaa gcgctcccgc tcgtgcgcgg cttcgtcgac acgtttgcgg catacgacgc
     3781 catcgtcgcc ccgaccgggt cgtgcgtcgg cgcgatccgc caccagcacc acgacctggc
     3841 cgctgcggcg ggcgatgtcg ccctcacccg cgcggtcgat gcgctgacgc ccaaggtcta
     3901 tgacctgtcg gagttcctca tcgatgtcct cggggtcacc gatgtcgggg cctacttccc
     3961 acatcgggtg accttccatc ccacgtgcca ctcgatccgg gtcgccgggg tgggtgaccg
     4021 gccgcaacgg ctgctgcagg cggtgcgtgg gctgaccctc gtcgacctgc cgcacaagga
     4081 ggagtgctgt ggctttggcg ggacgttctc gctgaagaac gccgacgtgt cgatcgcgat
     4141 ggccagcgac aaggcgcggc acgtccggga gaccggtgcc gaggtgctcg tcgccggcga
     4201 caacatgtgc ctgatgaaca tgggcagcgt gctggctcgc cagcgggccg gggtgcgggt
     4261 catgcacctg gccgaagtgc tcgcctcgac cgaggaggac ccggcatgac gaccacctac
     4321 ccgagcgggc cgacctcggc accgacctct gggccgctgc aagcgacgac ggcgaccttc
     4381 atcgggatgc cgaagttccc caaggcggcc aaggaggcgc tggccaacac ccagcagcgg
     4441 cgcaacctcg cccacgcgac gggggtgatc cgggccaagc gggccggggt cgtcgccgag
     4501 ttgccgcagt gggaggacct gcgcctggcc ggcgcggcgg ccaaggacga gacgatgacc
     4561 catctcgcgg catacctcga gcagttggaa gccaacctcg tgcgcaacgg ggcgcaggtg
     4621 cactgggccc gcgacgcggc ggaggcctgc cagatcgtcg tcgacatcgc tgcggccaag
     4681 ggggtcgacg aggtcgtcaa ggtcaagtcg atggcgaccc aggagatcga gctcaacgag
     4741 gccctcgaag ccgccgggat ccacgcctgg gagaccgacc tcgccgagct catcgtccaa
     4801 ctcggtcacg accggccgtc gcacatcctc gtcccggcga ttcaccgcaa ccgctcggag
     4861 atccgcgaga tcttccggcg cgagatgcac ctggtgggac gcccggctcc tgaggacctc
     4921 accgacgagc cgcgccggct cgccgaggca gctcgcctgc acctgcggga gaagttcctc
     4981 cgggcgaagg ttgccgtgtc gggggccaac ttcgcgatcg ccgacacggg caccctcgtc
     5041 gtcgtcgagt cggagggcaa cggccgaatg tgcctcaccc ttcccgagac gctcatctcg
     5101 gtggtcggga tcgagaaggt gttgccgacc tggcaggacc tcgggacctt catgcagctg
     5161 ctcccgcggt cctcgaccgg ggagcggatg aacccctaca cctcgatgtg gaccggagtg
     5221 catcccggcg acgggccgca ggaggtccac gtcgtcctca tcgacaacgg acgctcgcac
     5281 gtgctcgccg accccttggg ccggcaggcg ttgcgctgca tccgctgctc ggcctgcctc
     5341 aacgtgtgcc ccgtctatga gcgggccggc gggcacgcgt acggctcggt ctatcccggg
     5401 ccaatcggcg ccgtcctcaa cccgcaactg cgtggcacgg cgagcgatgt cgacaagtcg
     5461 ttgccgttcg cgtcgacgtt gtgcggtgcc tgcttcgagg cctgcccggt gcggatcgac
     5521 atcccgtcgc tgttggtcca cctgcgggcg caggtcgtcg acgagtcggg tcgaaagtct
     5581 gcggaggcca aggcgatgaa ggcgatggcc tggatgatgg gcgactatcg ccgactcgaa
     5641 aaggcccaga aggcagcgac attgggcggg cggctgctca agggccgcac gatccacacc
     5701 atgcccggcc cgttcgcacc ctggaccagc gcacgggacg tgccgtcccc acccaccagc
     5761 tccttccggg actggtggaa ggcgcgaaac acggattcca cgacggagga ccagcgatga
     5821 cgacgaccag ccttccggcc gccgacagtg cggctcgcac cgccgaacag ttgcgcaccg
     5881 ccgaacagat ccgcaacgac gcggcgcggg cggccatctt cggtcgagtc gcggccgcag
     5941 tcaccgacat ctcggcgccg ttgggtgccc gcggcgacgc tcctcgcatc gagcagccgt
     6001 cgccagggcg ggcgcagacg gtggagttgt tcatcgagaa cgtcgtcgac taccgggcca
     6061 ccgtcgtccc gatcgcgcca ggcaaggtgg gctcggccat tgccaaggcc ctcaaggcgg
     6121 ccacctcggt ggttgttccg tccgggctcg acccggcctg gaccgcgggg ctgccggacg
     6181 gcatacgcat cgtctccgat gaccacctcg acaacgcggc cctcgacggg gtggcggcgg
     6241 tcgtgactgc cgcgaccgtc ggcgtggcga cgacgggcac gatcgtcctc gaccatggtc
     6301 ccgaccaggg gcgccgggcg ctctcactcg tgcccgacct gcacgtgtgc gttgtgcgtg
     6361 cgtcgcaggt cgtgcacgac gtgcccgagg cggtggcgcg gctgcggtcc acggtggatc
     6421 aaggcgcgtc agccggtatg ccgcgtccgc tcacctggat ctcggggccg agcgcgacca
     6481 gcgacatcga gttggagcgg gtcgaagggg tccacgggcc gcggaccttg cacgtgatcc
     6541 tcgtgcagga ctgagccgct gggggcctgg gtcctccctg cgcggtggcc ccatcccgga
     6601 caagtcggcg agtgtcgagt tgccgggggc tggatccgtg tcgggcgtcg ggcaagtcgg
     6661 cgagtgtcga gttgccgggg gctggatccg tgtcgggcgt cgggcaannn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnngtc ggcgagtgtc gagttgcccg gggccaggcc
     6841 ggtcagcgga aggcggcctg gccggtcagc gcctgcccga tgacgagctg gtggacctcg
     6901 ctcgtgcctt cgtaggtgag caccgactcg agattgttgg cgtgccgcag cacggggtag
     6961 tcaagcgtga ttccgttggc gcccaacagg gttcggcatt cacgggcgat cgcgatcgac
     7021 tcccgcacgt tgttgagctt gccgaggctg acctgctcgg ggcgcagcgt gccggcgtcc
     7081 ttgagcttgc cgaggtgcag ggcgaggagc attcccttgc ccaactcgag cgtcatgtcg
     7141 gcgagcttgg tctgggtgat ctgatagccc gccagcggct tgtcgaagat gtcccgggtg
     7201 gcgacatact cgagtgtcgt ctcgaggcag tcgcgggccg cgccgagggc gccaaagatg
     7261 atcccgaacc gggcctcggt gaggcacgac agcgggccgg agagaccctt ggccagcggc
     7321 agcatcgccg acgcaggcag ccgcacctgg tcgaggacca actccgaggt gaccgacgcg
     7381 cgcaacgaca acttgccctt gatcgcggga gccgagaagc ccggcgaggc ggtcggcacg
     7441 atgaacccgc gaatcccctc gtcggtctgc gcccagacca cggcgacgtc ggccaccgac
     7501 ccgttggtga tccacatctt ggtgcccgtg agcacccagt ccgcgtcgtc gccggagccg
     7561 tcgcgggcgg catacgtgcg catgcccgcc gggttggagc cgaagtcggg ctcggtgagc
     7621 ccgaagcaac cgatcgcctc gccggtagcc atccggggca accactcctg cttctgctcc
     7681 tcgctgccga agcgatagag cgcgaacatc gccagcgatc cctgcaccga cacgagggag
     7741 cgcacgcccg agtcgaccgc ctccaactcc acgcacgcga gcccgtatgc cgtggcgctc
     7801 gtccccgcgc agccgtagcc ctccaagtgc atccccaaca ggcccaacgc gccgagctcg
     7861 acggccagct cgcgcgccgg gatggtgccc gactcgaacc actcgccgat gtgcgggcgc
     7921 acctttgccg tgagcacggc ccggacggtg tcgcggatgg cccggtcctc ggcgtcgatg
     7981 agagcgtcgg tgccgaacaa tccgaggggg gtctgggggg tgcggtcggc catgatcggg
     8041 cttcctgttc cggtggctgg gcttgctcgt gcgggtggtg ggcgtggtcg ttctgtcagt
     8101 atgccgcgtg ctcagctcat cgtcaggccg ccggaaacgc tgagggtctg gccggtgatg
     8161 taggcggcct cgtccgaggc gaagaaggcg acggcgttgg cgatgtcttc gggagcagcc
     8221 aagcggcgca acgggattgc ccgggtgaga ccctcgcgta acttggggtt gtcgccgccc
     8281 atcgaggcga acaacgcggt gtcggtgggg cccgggcaga cgacgttgac ggttacctgg
     8341 tggcgggccg actcgcgggc gatggtcttg gagaaggcga tgatcgcgcc cttggctccg
     8401 gaatagaccg cctcgccgct ggacccgacc cgcccggcgt cggagccaag gttgacgatc
     8461 cggccgcggt tctgggccgc catgacgggc aggacctcac ggcaggtgtt gaggaccccg
     8521 acgtaattga tgtcgatgac cttgcgccac aggtctggct cgctgtcgac gaacggcacg
     8581 gccttgtccc aaccggcgtt gttgaccaag acgtcgatcc gcccatgagc agcagtgacg
     8641 gcggcgacca tggcggcgac cgatgcgcgg tcggtgacgt ccacggcata ccaactggcg
     8701 gagccgccga tctcggtcgc gacggcttcg gcggcgggac cgtcgaggtc ggtgacgacg
     8761 acgatcgcgc cctgggcggc ggtgacccgg gcgatggcgc ggccgatacc cgcaccggcc
     8821 ccggtgacga tggtgacctt gtcctgcaag cgcatggggt gggtcctttc ggtggggacg
     8881 ggtcagggcg cgaagtcgcg gccgagcagg tggcgggaga tgacgagctt ggagacctga
     8941 gcggtgccgt caccgatctc gaggccgatg acatcgcgca gccgttgccc gatggggcag
     9001 tcgctggagt aggcgacgtg gccgacggtg agcagacatt gatggatgac ctcggtggcg
     9061 agtttgggtg cccagaactt cgccatcgcc gcctcggcgc tgtggtcgag gccagtgtcc
     9121 ttgcgccaca acgcttccag gcagatgtgc cgcgcgccct tgagataggt ggcgcactcg
     9181 gccagcggga aggccacccc ttggaaggtg ccgatcggct ggccgaaggc gatccggtcg
     9241 cgcgaccact gcagggcctc gtcgagggac gcctcggccg cgccgatgca gcccaggccg
     9301 atgatggccc gggagtagtc gaagccctgc atgaccgaca caaagccgcc gccctcggca
     9361 ccaatgaggt cgtcgtcgcc caccggggtg ccgtcgaagt gcagtgcggc gcgcccgatc
     9421 gcccggctgc cgaggtcgtc gaagggcgag cgggtgactg tcgggtcgtg caggtcgacc
     9481 cagaaggcgc tgacgccgcg cgctccgggg ccgccggtgc gggccaggac gaccgcgcgg
     9541 tcggaggcca tgccgagggt gatcgaggtc ttctcgccgt gcagagtcca accggtcgca
     9601 gtacgaatgg ccttgagctc caggtttgct gcgtcggtgc catggccggg ctcggtgatg
     9661 cagatgcacg ggactgtggt gccggctgcg atcccgggca gccacgcggc ctgctgctcg
     9721 ggcgtcgcgt tgcggacgat gatgtcgctg acgagcgccg tgttgatgat gacgtaggtc
     9781 gcgttgaagt cggcccgccc gacttcctcg gtggcgatac cggcgatgac ggcgctcgcg
     9841 tcctggccgc cgtaagcctc cgggatgcgc aggccggtca ggcccatggc cgccaggtcg
     9901 agggcctgct cgcggcggaa ggtcgcagtc ttgtcatccc gctggtagtg gggagcgagt
     9961 gccttggtcg cgaactggcg gaccgcctgg cggaagtcct cctgctcgct gtcgagcccg
    10021 taatccatga gcgcgcctca gtgccagttg acgtggtggt tgaagtcggg ggtgcgcttc
    10081 tcggcgaagg cggcagcgcc ctccagaccc tcgggggagg cggtgaacag gtcgagcgcc
    10141 gacatggcga ggttcgacag gcccgcgtgg tggtcggtgt cggcgttgaa cgactgcttg
    10201 aggaagcgga tcgccgtcgg gctcttggtc gccacctcgt cagcccacgc ctttgcctcc
    10261 gccatgacct cgcccggagg cacgaccttg ttgacgaggt tcatcgctag ggcctcctgg
    10321 gcggagtaca tccggcacca gaaccagatc tcgcgcgcct tcttctcgcc cacgacgcgc
    10381 gcgaggtatg ccgtgccgaa ccccgcgtcg aaggatccca cccgggggcc ggcctggccg
    10441 aagcgggcgg tctcgctggc gatcgacacg tcgcacagga cgtgcaggac atgcccgccg
    10501 ccgacggcca cgccgttgac cgcggcgatg accggcttgg ggatgtcgcg aatcaccttg
    10561 tgcagtgcgc cgatctcgaa catccccgac tcgctcgggc cgtagtcgcc ggtctcggca
    10621 cgttgcttga cgtcgccacc cgtgcagaag gccttgtcgc cggcgccggt gaggatgacc
    10681 gactggacct ggcggtcggc ccaggccaac cggaacgcct tgatgagttc ttcgacggtc
    10741 tgggcgcgga aggcgttgta gcgctccggg cggttgatcg tcacgatcgc cgccgagtcc
    10801 tgcacgacgt aggtgatgtc ctcgaacggc gtcatcgtcg gttctcctgg ctccgtgctg
    10861 aacggctatt cattgaatgg tcgttcaatc acgggtatgc tgtcaagggt gagctcagcg
    10921 gacccgtccc gcgaggcccg ggccgaggct cgggtcgaga cccgtcggcg gcagatcctg
    10981 cagtcggcga cccggatcat gatccgcacc ggctatcacg agatgtcaat gcaggccgtg
    11041 gccgacgacg ccgggatgag cgtggggctg atctatcagt acttcggcgg caagcaggag
    11101 gtgttgcggg ccgtcatcgt cgacatcctc gacaccttcc gggccgaggt gccggcggcc
    11161 atggcggcgg ccggcgctga tccggaggct caactggcag ccgggttcgc cagcttctgc
    11221 cgggtcatcg acgccaagcg ggccgggacg atgctggcct atcgcgagag ccagaccctc
    11281 gatgccgccg ggcaggccga gatcatccgg ctggagcagg agaccatcga cccgttccgg
    11341 gtcgccgtcg aggcgggcat cgctgcaggg gtgttccggc cggtccctgc cgagttggtc
    11401 gcccacaacc tcaagctcgc cgcccacggc tgggccctca agcactggga tgtgggcgcg
    11461 cgactggacc tcacggcata catcgatgcg gagttagacc tcctgctcgc cgccatccgc
    11521 gcctgacccg gcgggggagg ggcaagcagc gacccggcta ctcgtcgcgc caggagcgcc
    11581 agagggcggc atacgcccca ccggcggcga tgagctcgtc atgcgagccg atctcggtca
    11641 ccaacccgcc atcgacgaca gccacccggt cggcgtcgtg agccgtgtgc agccggtggg
    11701 cgatcgccac gacagtgcgc ccggtaacga ccgcggccag cgagcgctcc agatgccgcg
    11761 ccgcccgagg gtctagcagc gaggtcgcct cgtcgagcac caacgtgtgc gggtcggcga
    11821 gcaccagccg agccagcgcc aactgctgcg actgggccgt cgtcaacgag tgagcaccgg
    11881 acccgacccg ggtgtccagc ccgtcggcca gccctaacgc ccactcgcgc ccgtcgacgg
    11941 cggtcaacgc gtccaacaac tcgtcgcgac tagccgaggg ccgggccagc agcaggttgt
    12001 ccgccaacgt cccgacgaac acgtggtgct cctgcgtcac caaggccacc tcaccccgca
    12061 accggtcgag gtcgaggtcg accaacctca ccccgccgac atcgacccga ccgagccgcg
    12121 gcccgtcgat ccccgccatg agccgcccga gcgtcgactt gccggccccc gacggcccga
    12181 cgatcgccag ccgctcccca ggggcaaggt ctagcgacac tccgcgcagc acgtcgcgcc
    12241 cggcgcgata ggcgtaggag acgtcgtcga cgaccaggtg cgcatccacc ggcgtctcac
    12301 cggtcgcctc acggtccggc gggacgtcgg cgatcccgat gacccgggcg agcgacgtcg
    12361 ccccaacctg gatctcgtcc agccacatga gcaggtcgtc cagggggttg gccagttggt
    12421 ggacatagag cgccaccgtc gtcacggccc cgatgctcgc gtgcccctgc gagaccagcc
    12481 aaccgcccca gaccagggtc gccgccaccg gcaacagata cgcgaactcg acctggggga
    12541 accagagcaa ccgcagccgc agggtgtaca tctcggcgtc gaacgatgct cgcaacgcct
    12601 cgtccatccg gcgccgacgc cgggccgaca acccgagcgc atcgacggtc cgagcgccgt
    12661 cgacagtctc ggtgacgacg ccgttcatca cggcatacgt cgcgcgctcg cgcagataac
    12721 cgtcggg
//
LOCUS       DAOMZY010000055        12660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_4587_length_12660_cov_5.490758, whole genome shotgun sequence.
ACCESSION   DAOMZY010000055 DAOMZY010000000
VERSION     DAOMZY010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 12660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12660
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4587_length_12660_cov_5.490758"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            414..3926
                     /locus_tag="PLK64_04790"
     CDS             414..3926
                     /locus_tag="PLK64_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPV79092.1"
                     /translation="MTADLAALGARLRLLTDAQLRAVAGPTTFTRGQGYAAQRRVSGL
                     AASSTWVVSAKVRGSGRIYQTQVELYLSRGEVLWTGECTCPMQVDCKHSVAVAIVARQ
                     TFGLAGAATLATPAAYVSDSDLARWAVVPALDEPASWTDQPPPDEYDRYDYDDDGYPD
                     PLAALHHLHPGAPTTPGLPAWERAVRAVVGDTAATTTHFTPVGLLFEVQVTPPNRYSP
                     PGSAHAKLLVRPMVPGKTGWVRTGLTWGNLDYASPYGQIKLNPVHQSALQAVVAIARQ
                     PYGYRPIADRVDLAVLGRGWFAALRECQRLGIKLLTDPKRSGEVTLAEEPARVVVHLT
                     SDGEGGLLVGPTIVEPAEVAATQGDWEAIGSPASGMWVAHGDDLVIAAFEPAVDVPTQ
                     RWLVEGGQRVPAGDVARFFAGHLPALAKRFDVLSPDGSVEVPTVGAPRLLLRLTHEPG
                     LRLSLAWSFTYAVGQDRVEVAVGSSTEPTIRDLAAEERLIDGLDALDKAPGLRTARPT
                     VGWSLQPRSGLDGSPMLRFLNLVLPRLEEHPDVDIEVLGTPIPYAEADEAPTVSLAIT
                     ESSDSTDWFDLDVQVRVGPQDVPIRSLVTALTLGEEHVILPSGTWFRVDRPELTELRR
                     LIDEARAMSDDPKGPLRLSAYQADLWAELEELGVVQEQSERWRSLVAGLAGAAEGAPP
                     EPVPAPDGLAAELRHYQHSGYEWLTFLRRAGLGGILADDMGLGKTMQTLAMILRAVQD
                     REEAADAEGATGAGGERAAYPPFLVVAPTSVLPTWLAEAARFAPSLRVVALGETTKRR
                     RGSIAEAIAGADLVVTTYAVFRIDADAFRECAWSALVLDEAQFVKNHQSQVYQCARLL
                     PARTKLAITGTPMENNLMELWALLSIVAPGLFPRPAAFAEHFRRPIESGTAPERLDTL
                     RRRIRPVMLRRTKDAVASELPPKQEQVIEVALSPKHRRIYDRVLAHERQRILGLLDDA
                     QRNRIAILRSLTRMRRLCLDPVLDDPAHAGIGSAKVDALLELLTPVLAEGHQALVFSQ
                     FTGFLATVRARLDAEGISYAYLDGRTRKREEAIASFRSGERSVFLVSLKAGGFGLTLT
                     EADYVFLLDPWWNPATEAQAIDRTHRIGQDKHVFVYRMVAADTIEDKVLALQERKREL
                     FARVVDEGALASGVLSPEDIRGLL"
     gene            3976..4209
                     /locus_tag="PLK64_04795"
     CDS             3976..4209
                     /locus_tag="PLK64_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HPV79093.1"
                     /translation="MSTQIAVRLPDEMVAFLDRAVASGVATSRAAVVASAVEREMRRQ
                     AAERDADILRRQGTEDDLDGLVAWTVDHVAIED"
     gene            4212..4526
                     /locus_tag="PLK64_04800"
     CDS             4212..4526
                     /locus_tag="PLK64_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017825004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HPV79094.1"
                     /translation="MREIALVRLDKTRPALVLTRNAARGSMTKVTVAPITSTIKGLSS
                     EVLLGPANGLDHECAAALDNVVTIPTTLLGRTVGYLTAAQESHLARAVVLAYDLDVPL
                     FE"
     gene            4523..5176
                     /gene="idi"
                     /locus_tag="PLK64_04805"
     CDS             4523..5176
                     /gene="idi"
                     /locus_tag="PLK64_04805"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HPV79095.1"
                     /translation="MTDVSPMFVEPSPVPADLSGELVVLVDDGGAAIGVAPKASVHGA
                     DTPRHLAFSCYVIDASGRLLVSRRATTKPTFPGVLTNSLCGHPGPGESLPDAVCRRAG
                     SELGLDLAGWPIRLVLPQFSYAAEMNGVVENEACPVFVVTVPDGIPLAPDADEVASVE
                     WMPWADYRDRVLDGSLQVSSWSADQVRQLVALGADPATWPEAPASDLPPAAQPHPAG"
     gene            complement(5193..6188)
                     /locus_tag="PLK64_04810"
     CDS             complement(5193..6188)
                     /locus_tag="PLK64_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV79096.1"
                     /translation="MGIDKTDDSAMAQQYAVVAASQRLGRPFYTPQPQASSIADLRHD
                     DPGERCDLWGLYVGPDMVGTVTLWLPLHDNTDTVWLQLDVHPDHRGRGYGGLAVAAVV
                     DFARDLGRTRVVTTARYPADRPVDHPYRRFAEQHAFRLGSLDVNRRLPLPVPAPVLSG
                     LAERARAAYLPAYRIETFTRVPDALLAPLCACMSRVESDAPSGELEWEEEALTPERLR
                     GFAELDEHVGRLRITSVAREAESGEVVGYTELFIIGGTTKALQSGTFVVSEHRGHRLG
                     LGLKVANLVALQAHRPDAVEIFSINNAENTWMVAVNEELGFEPVELVGQFYRQLP"
     gene            6250..6714
                     /locus_tag="PLK64_04815"
     CDS             6250..6714
                     /locus_tag="PLK64_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79097.1"
                     /translation="MRRTIWLVGVMGIWVLALGACATSVSGPASAPSSGSLDPATLRD
                     QLRSAAASLKPTAGYVPTPAASGLDPALGAYVAIARSDLAARLSIAPDRIGVATARVV
                     TWPNAALGCPKPGMAYADQVIDGALIILRVDERSYEYHSGGDTAPFLCETVG"
     gene            6800..9805
                     /locus_tag="PLK64_04820"
     CDS             6800..9805
                     /locus_tag="PLK64_04820"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HPV79098.1"
                     /translation="MFVAVLIQHPEDGMRRRIAGLAALSLAVAPLVTTLGALSAAAAP
                     QKKSYIVVMKADPLVRSMSAKSLGTPTAKSRKGDLRRVQDKVLAAAGLPASAKSTSYT
                     NALNGFAVRATDAQVDSLSKRPEVAAVLVDELRQPTAHDTSRSIAVDRGGPGNRLYDF
                     LGLDGRSESWRSGLTGAGVTVGVIDTGIWPEHPSFADDGSYPVHAPLDTSVGSACAFG
                     NTAHNPGDAAFTCNNKLVGARVFLDTYRANSGLTAEEFDSARDNEGHGTHTASTAAGN
                     ANVQAQILGVDKGMTSGIAPRAQVIAYKALGEGGGYTSDLVAAIDQAVADGVDVINYS
                     IGGGAATVAADTLAFLFAADAGVYSAVSAGNSGPGAKTIGGPADVPWVTSVGANTQPT
                     FYQGTIEAGGKRYLGASVTKGVGPAKFVDAAAAGSELCLEGQLNPAKVAGAIVLCKRG
                     GNGRVAKSAEVLRAGGVGMVLFNAVDVDTLFSDTFFVPTVMIDFTPGTQLKAWIAGTP
                     NPVGRLVSGGIGTSPFYSPTMAYFSSRGENPTAGDIIKPDITAPGVQIMAGNTPKPSG
                     NSQPSGELFQAIAGTSMSSPVVAGSYALLKQAHPTWSPAAAKSALMTTANTAVRDNDR
                     VSAAGPFAMGSGMVDLGEPRSRGSAYQPGLVYDAGFTDYLGFLCAEGPSVFGNPAATC
                     ASLAASGIPTRAADLNLSSIGVEAVAGSTTVTRTVTSVADRRTSFEAKVQAPAGYRVT
                     VTPSRITLAPGASATFTVTITNTGSAPAGQWRTGALTWRGDGYEVRSPIAVKGTTLSA
                     PTSVSGTGTSGSASLALQFGFAGPYTATAEGLLAATHTPGTIAQDPDTTYPSPDDGAG
                     VVSVPFTLSGVGVAKWALNMPDPTDLDIYLFGPGGNLVGASTNGGTKESILLNSPADG
                     TYTMVIHGWAVGATPLQFDLRSWLVPAEDGAGSLSISSGASTTATIGGSHTVGIAWTG
                     LTAGEEYFGSVTHRNGTAVVGRTVVSVTG"
     gene            complement(9927..10646)
                     /locus_tag="PLK64_04825"
     CDS             complement(9927..10646)
                     /locus_tag="PLK64_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79099.1"
                     /translation="MITYSRRSFRLYLANRQYVLAVPIYTLGLMLLLSILIVIVVGIA
                     TGFPVSKDFEEGFRANSGAILSLPGFLVSVGVLAATRNFAMALAFGSTRRHFWLGTTA
                     GFVVTAAVTAAAAVLMLGLERLTGHWFVGAYAFDVAVLGNGNIAQTFSVTFVLALLSL
                     TVGALFGTVLRAFGTKATAVLSIGVLLVLFAAVALVVWKWSVVAPLLANWGAWATVII
                     GAAFVVLASISSYAACRRATV"
     gene            complement(10643..11710)
                     /locus_tag="PLK64_04830"
     CDS             complement(10643..11710)
                     /locus_tag="PLK64_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV79100.1"
                     /translation="MSTPTAGPATGTATAGTPTGIATRITTASAGITLRGLTKSYGTT
                     EVLVDVSATLEPGRIYGLLGANGTGKTTLLACIAGHTFRDGGEIRIDGHDPLEHAPTL
                     ARLCFVHEDQAYNDAFSVADILAVMPTFYPEWDDETATRLMRRFRIPRRTRSRKLSRG
                     QRSALAIVLALASGAAYTFLDEPYLGLDATARGIFYDELLAVYAASHERAADGTTAPR
                     TFIMSTHLIDEAAELIEEVLVLDRGRIVLQADVEDARRTAFLARGFEADVLALAAGRE
                     LLSTRRLGGIVTATICGELSSDERQRAEAARVSIEPTTLQEFVAAIGQHGESDQPAPH
                     LAPHPAPLPASQSASESRSLK"
     gene            complement(11710..12069)
                     /locus_tag="PLK64_04835"
     CDS             complement(11710..12069)
                     /locus_tag="PLK64_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPV79101.1"
                     /translation="MTDWTDGRPIFMDLADRIAADILAGLIPEETQVPSTNELAAHYR
                     INPATAGKALNRLVDQGIVYKRRGLGMFVAAGAPEALRAQRRDAFSEQFVRPFVEEGR
                     RLGLSIDEIVALVTKER"
     gene            12176..>12660
                     /locus_tag="PLK64_04840"
     CDS             12176..>12660
                     /locus_tag="PLK64_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT domain-containing protein"
                     /protein_id="HPV79102.1"
                     /translation="MSPVDIEEAVRRYAASWPSLVPVADAFVTTVTGILDDAGINYLT
                     VTGRAKAVASFAAKAARERDGARAHPDPMRDITDQVGVRVITYVRSDVDAVAELLADQ
                     LTVLDDRDMGRETARAGRFGYASRHLLVSLDSGEHTAYEPTRCASIQLRTVLQHAWAE
                     F"
BASE COUNT         1967 a         4453 c         4280 g         1960 t
ORIGIN      
        1 cgccgatgac ttcctgcccc cagccgccgc ccgaacccca cttccccgca ctcgccgatg
       61 acttcctgcc cccagccccc gcccaacccc cagaacctca cacacgccag cgatttcgat
      121 gctgccgccc ggatctatcg ccgatgccgg gctgctggcg tgaccccgag agggctgatc
      181 gattgcctca tcgcagccgt ggcctggcgt cacgaggcgc cgttgctcgc ccatgacgcc
      241 gacctggccc ggatcgctgc cgtcatcgga gccccactgg acccggcgtc gatcgggccg
      301 tgacgagaag tcctcaccac gaacgcaccg cggccgatgc ctgtggatag cgcggggtgg
      361 gtgtcggcgg gacggcatac agtgctcggg tccgtatcac ttcgggagcc gtgatgaccg
      421 cagacctggc cgccctcggg gcgcgactgc gtctgctcac cgacgcgcag ttgcgtgccg
      481 tcgccggtcc gacgacgttc acccgcggcc aggggtatgc cgcgcagcgc cgggtttccg
      541 ggctcgcggc gagcagcacc tgggtggtga gcgcgaaggt tcgcggttcg gggcggatct
      601 atcagaccca ggtcgagctc tatctcagcc gcggcgaggt gctctggacc ggcgaatgca
      661 cctgtcccat gcaggtcgac tgcaagcact ccgtcgccgt cgccatcgtc gctcgccaga
      721 ccttcgggct ggccggagcg gccaccctcg ccacccccgc ggcatacgtg tccgattcgg
      781 atctcgctcg ctgggcggtc gttccggccc tggatgagcc ggcctcgtgg acggatcagc
      841 caccgcccga cgagtacgac aggtatgact acgacgacga cggctacccc gacccgctcg
      901 cggccctgca ccacctccac cccggcgcgc cgaccacccc gggcctgccg gcctgggagc
      961 gggccgtgcg cgcggtcgtg ggcgacacgg cagcgacaac gacccacttc accccagttg
     1021 ggttgctgtt cgaggtgcag gtcaccccgc ccaaccgcta cagcccaccc ggctccgccc
     1081 acgccaagct gctcgtgcgc ccgatggtcc ccggcaagac gggctgggtc cgcaccgggc
     1141 tcacctgggg caacctcgac tacgccagcc cctacggcca gatcaaactc aaccccgtcc
     1201 accagagcgc gctgcaagcg gtcgtcgcca tcgctcgcca gccctatggc taccgaccga
     1261 tcgccgaccg ggtcgacctg gcggtgctgg ggcgcggctg gttcgccgcg ctgcgcgagt
     1321 gccagcgcct cggcatcaaa ctcctcaccg accccaagcg cagtggcgag gtcacgctgg
     1381 ccgaggagcc agcgcgcgtc gtcgtgcacc tgacgagcga cggcgagggc ggtctcctcg
     1441 tcgggccgac gatcgtcgaa cccgcagaag tcgctgccac ccaaggcgat tgggaagcga
     1501 tcggctcacc tgcgtccggc atgtgggtgg cacacggcga cgacctcgtg atcgccgcct
     1561 tcgagccggc ggttgacgtg ccgacccagc gctggctcgt cgagggcggg cagcgggtgc
     1621 cggcaggcga tgtggcgcgc ttcttcgccg gccacctccc cgccctggcc aagcgcttcg
     1681 acgtgctctc gcccgacggc agtgtggagg tgccgacagt gggcgcgcct cgcctcctcc
     1741 tgcgactcac ccacgaacca gggctgcggc tctcgctcgc ttggtccttc acgtatgccg
     1801 tggggcagga ccgcgtggag gtcgccgtcg ggtcgagcac cgaaccgacg atccgcgacc
     1861 tcgctgccga ggagcggctg atcgacggcc tcgacgcgct cgacaaggca cccggcctgc
     1921 gcactgcccg gccgacggtg ggctggtcgc tgcagccccg ctcgggcctg gacggctcgc
     1981 cgatgctgcg cttcctcaac ctcgtgctcc cccgcctcga ggagcatccc gatgtcgaca
     2041 tcgaggtgct cggcacgccg atcccctatg ccgaggccga cgaggccccg acggtcagcc
     2101 tggcgatcac cgagtcgagc gactcgaccg actggttcga cctcgacgtg caggtgcggg
     2161 tcggcccaca ggacgtgccg atccgcagcc tggtcacggc cctgacgctc ggcgaggagc
     2221 acgtgatcct gcccagcggc acgtggttcc gggtcgaccg acccgagctg acggagctgc
     2281 gccggctcat cgacgaagcc cgggcgatgt ctgacgaccc caagggtccg ctgcggctct
     2341 cggcctatca ggccgacctc tgggccgagc tcgaggagct cggcgtcgtc caggagcaga
     2401 gcgagcgctg gcggtcgctc gtggccgggc tcgcgggtgc ggccgaaggc gccccgcccg
     2461 agcccgtgcc cgcccccgac gggctggcgg ccgagctacg gcactaccag cacagcggct
     2521 atgagtggct gaccttcctg cgccgggccg ggctcggcgg catcctcgcc gacgacatgg
     2581 gtctgggcaa gacaatgcag accctcgcga tgatcctgcg cgcggtgcag gaccgcgagg
     2641 aggcggccga cgcagagggc gcgacgggcg cgggaggtga gcgggcggca taccccccgt
     2701 tcttggtggt ggcccccacc tcggtgctgc cgacctggct cgccgaggcc gcgcggttcg
     2761 caccgtcgct gcgggtggtc gcgctcggcg agacgaccaa gcgccgccgc ggcagcatcg
     2821 ccgaggccat cgcgggggcc gacctcgtgg tgacgacgta tgccgtgttc cgcatcgacg
     2881 ccgacgcctt ccgggaatgc gcgtggtcgg cgctcgtgct cgacgaggcg cagttcgtca
     2941 agaaccacca gtcgcaggtc tatcaatgcg cgcggctgct gcccgcccgg accaagctcg
     3001 ccatcaccgg cacgcccatg gagaacaacc tcatggagct gtgggcgttg ctgtcgatcg
     3061 tcgcgccggg gctgttcccg cggccggccg cgtttgccga gcacttccgg cgaccgatcg
     3121 agagcggcac cgcgccggag cgcctcgaca cgctgcggcg gcggatccgg ccggtcatgc
     3181 tgcgacgcac caaggatgcc gtggccagcg agctgccccc caagcaggag caggtcatcg
     3241 aggtcgcgct ctcgcccaag caccgccgga tctatgaccg ggtgctcgcc catgagcggc
     3301 agcggatcct cgggctcctg gacgatgcgc agcgcaaccg gatcgccatc ctgcggtcgc
     3361 tgacccggat gcggcggctg tgtctcgacc cggtgctcga cgacccggcc cacgcgggca
     3421 tcgggtcggc caaggtcgac gcgctcctcg aactgctcac cccggtgctc gctgaggggc
     3481 atcaggcgct ggtgttcagc cagttcaccg ggttcctcgc gacggttcgc gcgcggctgg
     3541 acgccgaggg gatcagctat gcctacctcg atgggcgcac ccgcaagcgc gaggaggcca
     3601 tcgcgtcgtt ccggtcgggc gagcggtcgg tgttcctcgt gtcgctcaag gctggcgggt
     3661 tcgggctgac cctgacggag gccgactacg tcttccttct cgacccgtgg tggaaccccg
     3721 cgactgaggc acaggcgatc gaccgcaccc accggattgg gcaggacaag cacgtcttcg
     3781 tctatcggat ggtcgccgcc gacacgatcg aggacaaggt gctcgcgttg caggagcgca
     3841 agcgcgagtt gttcgcccgg gtcgtcgatg agggagccct agccagcggg gttctgagcc
     3901 ccgaggacat ccgtggcctc ctctgagcct gcctatgcat cccacgctgg gatgcatttc
     3961 ggtatgctca cgactatgag cactcagatc gccgttcgac tgcccgacga aatggtcgcg
     4021 ttcctcgatc gcgccgtcgc ctcgggagtg gcaaccagtc gggccgcagt tgtcgcttcc
     4081 gcggtggagc gcgagatgcg ccgacaggcc gccgaacgtg acgcggacat cttgcggcgg
     4141 cagggaaccg aagacgatct tgacggcttg gtggcttgga ccgttgacca cgtcgcgatc
     4201 gaagactgac agtgcgcgag attgccctag ttcgcctcga caagactcgc ccagccctcg
     4261 tgctcacccg gaacgctgcc cgcgggtcga tgaccaaagt gaccgttgcc cccatcacct
     4321 caaccatcaa gggactgtcc agcgaggtgc ttcttgggcc cgccaacggg ctggaccacg
     4381 agtgtgcagc ggccttggac aacgtcgtca ccatccccac aacactgctt ggtcgcaccg
     4441 tcggctacct caccgctgcc caggagtcgc acctggcacg ggctgtggtg ctggcctacg
     4501 acctcgacgt tcctctgttt gaatgaccga cgtgtccccc atgttcgtcg agccgtcgcc
     4561 ggtgcctgcc gacctctccg gggagctggt tgtgctggtc gacgacggtg gcgccgcgat
     4621 cggggtcgct cccaaggcga gcgtccacgg cgctgacact ccgcgccacc tggccttctc
     4681 ctgctatgtc atcgacgcga gcggccgact gctcgtgtcg cgccgcgcca ccacgaaacc
     4741 caccttcccc ggggtgctca ccaactcgct gtgcggccat ccgggcccgg gtgagtcact
     4801 tcccgacgcg gtatgccgtc gggctggctc cgagctcggc ctcgatctcg cggggtggcc
     4861 gatccggctc gtgctgcccc agttctccta tgccgccgag atgaacggcg tggtcgagaa
     4921 cgaggcctgc ccggtgttcg tcgtgacggt gccggacggc atacctctgg ctcccgatgc
     4981 cgacgaagtg gcgagcgtcg agtggatgcc gtgggcggac taccgcgacc gagtgctcga
     5041 cggctcgctg caggtctcgt cctggtcggc cgaccaggtg cgacagttgg tggccctcgg
     5101 cgccgacccc gccacctggc ctgaggcgcc agcctccgac ctcccgcccg cggcccagcc
     5161 gcacccggcc ggctgacccc tcccgggagc gctcagggca gttggcggta gaactggccg
     5221 accaactcca cgggctcgaa gcccagctcc tcgttgaccg ccaccatcca ggtgttctcc
     5281 gcgttgttaa tcgagaagat ctcgaccgca tccggtcgat gtgcctgcag cgcaacgaga
     5341 ttcgccacct tgaggcccaa cccaagccga tgcccgcgat gttcgctcac cacgaaggtg
     5401 cccgactgaa gcgccttggt cgtgcccccg atgatgaaca actcggtata cccaaccacc
     5461 tcgccgcttt cagcctcgcg ggctaccgag gtgatccgca gccgcccaac gtgttcgtcc
     5521 aactcagcga acccgcgcag ccgctcgggc gtcagcgcct cctcctccca ttcgagttcc
     5581 ccactgggag cgtcggattc gacccggctc atgcacgcac acaagggcgc cagcaaggcg
     5641 tcggggacac gagtgaacgt ctcgatccga taggcgggca ggtatgccgc ccgcgcccgc
     5701 tccgccaggc cgctcagcac cggcgcgggc accggcagcg gcagccgccg gttgacgtcc
     5761 aggcttccca gacggaaggc gtgttgctcg gcgaaccggc ggtaggggtg gtcgacgggg
     5821 cggtcggccg gataccgcgc ggtggtgacc actcgggtcc ggccgaggtc ccgggcaaaa
     5881 tccactacgg cggccaccgc aagaccgccg tagccacgcc ctcggtggtc cgggtggacg
     5941 tcgagttgca gccacacggt gtcggtgttg tcgtgaagcg gcagccagag ggtgacggtc
     6001 cccaccatgt ccggaccgac gtataggccc cagaggtcgc accgctcccc cgggtcgtcg
     6061 tggcgcaggt cggcgatgga agacgcctgt ggctgtgggg tgtagaacgg ccgtccgagg
     6121 cgctggctcg cggcgacgac ggcatactgc tgcgccatcg cgctgtcgtc ggtcttgtcg
     6181 atgcccatga tctccacccc ggcaggctac tggcggaggt gttcgggttc gcgggagtta
     6241 gcctgaggta tgcggcggac catttggctg gtcggagtga tgggaatctg ggtgctcgcc
     6301 ctcggcgcgt gcgccacatc cgtatcgggc ccggcctcgg cgccttcctc aggctccctc
     6361 gatccggcca cattgcgcga tcaacttcgc agcgccgcgg catccctcaa gcccaccgcg
     6421 gggtacgttc ccacgcctgc tgcctcgggt ctcgatccgg cactcggcgc gtacgtcgcg
     6481 atcgcccgct ccgacctcgc tgcccgattg agcatcgccc cggaccgcat cggcgtcgct
     6541 accgcgcgcg tggtcacctg gccgaacgcg gcgttggggt gcccgaaacc aggaatggcg
     6601 tatgccgatc aggtcatcga tggggcgctc atcatccttc gcgtcgatga gcggagctac
     6661 gaataccact caggcgggga caccgccccc ttcctgtgcg agacagtcgg ctgaccttct
     6721 ccgacccaaa acggtggcgc ccggccctcc tcgggaatag ggtcgcccca cccgcacgga
     6781 actcgggata tatcgacccg tgttcgttgc cgtgctcatc cagcacccgg aggatggaat
     6841 gagacgacgg attgccggtc tggctgccct ctccctggca gtcgcacccc tggtcacaac
     6901 cctgggagcc ctgtctgcgg ccgcagcgcc gcaaaagaag tcctacatcg tggtcatgaa
     6961 ggccgaccct ctggtccggt cgatgtccgc caagtcgctc ggcaccccaa cggcaaaatc
     7021 gcgcaagggc gaccttcgcc gagttcagga caaggtgctc gccgcggcgg gactgcccgc
     7081 ctcggccaag tccaccagct acaccaatgc cctcaacggc ttcgcggtcc gggccaccga
     7141 cgcccaggtc gattcactgt ccaaacgccc tgaggttgcg gcggtgctgg tcgacgaatt
     7201 gcgtcagccc accgcgcatg acacctctcg ctcgattgcg gtcgaccggg gcggccccgg
     7261 aaatcggctg tacgatttct tgggcctcga cggacgctcc gagtcgtggc gctccggcct
     7321 cactggtgct ggcgtcaccg tcggagtcat cgacaccggg atctggcccg aacacccgag
     7381 tttcgccgat gacggcagct accccgtgca tgcgccgctc gacacgagcg tgggctcggc
     7441 gtgcgccttc ggcaatacgg cccacaaccc gggcgatgcc gccttcacct gcaacaacaa
     7501 gctcgtgggc gcccgcgtct tccttgacac ctatcgtgcc aacagcgggc tgactgctga
     7561 ggagttcgac tccgctcgcg acaacgaggg acacggaact cacacggcct cgaccgctgc
     7621 gggcaacgcc aacgtccagg cccagattct gggagtcgac aaaggaatga cctccggcat
     7681 cgctccgcgg gcccaagtga ttgcctacaa ggccctgggt gagggcggtg gctacacctc
     7741 cgacctcgtc gctgcgatcg accaggccgt cgctgacggg gtcgacgtca tcaactactc
     7801 gatcgggggc ggcgctgcga ccgtggctgc cgacacgttg gccttccttt tcgcggccga
     7861 cgctggcgtg tactcggccg tctcggccgg caacagcggc cccggagcca agacgatcgg
     7921 tggaccggcg gacgttccgt gggtcacctc agtgggtgcc aacacgcagc cgactttcta
     7981 ccagggcacc atcgaagccg gcggcaagcg ctaccttggc gcctccgtca ccaagggcgt
     8041 cgggccggcg aaattcgttg acgccgccgc cgccggaagt gagttgtgcc tcgagggtca
     8101 actcaacccg gccaaggtcg ccggcgccat cgtcctgtgc aagcgcggcg gcaacgggcg
     8161 cgtggccaag agcgccgagg tgctgcgggc cggtggggtg ggcatggtcc tgttcaatgc
     8221 ggtcgatgtc gacaccctct tcagcgacac gttcttcgtg ccgacggtga tgatcgactt
     8281 cacccccggc acccagctca aggcctggat cgccgggacg cccaacccgg tcggccgact
     8341 cgtctcggga ggcatcggga cgtccccctt ctactccccg acgatggcgt atttctcctc
     8401 gcgcggcgag aatccgacgg ccggggacat catcaagccc gacatcaccg cacccggagt
     8461 gcagatcatg gcaggcaaca cgcccaagcc atcgggcaac agccagccct cgggtgagct
     8521 cttccaggcg attgccggga cctcgatgtc aagcccggtc gtcgccggga gttacgcgct
     8581 gctcaaacag gcacacccca cctggtcacc cgccgcggcc aagtccgcac tgatgaccac
     8641 agccaacaca gcggtccgcg acaacgaccg ggtcagcgcg gccgggccgt tcgccatggg
     8701 ttccggaatg gtggatctgg gtgagccccg gtcccgaggc tcggcctacc agcccggcct
     8761 cgtctacgac gcggggttca cggactactt gggcttcctc tgcgccgagg gtccgtcagt
     8821 gttcgggaac ccggcggcga cgtgtgcgtc gttggccgcg agcggtatcc ccacccgggc
     8881 ggccgacctg aacctctcgt ccatcggcgt ggaagcagtg gccgggtcga cgaccgtgac
     8941 gcgcacggtc acgagcgtcg ccgatcgccg gacgtcgttc gaggccaagg tccaggcacc
     9001 ggcgggttac cgcgtgacgg tgactccgtc gcggatcacc cttgcgcccg gggccagcgc
     9061 gaccttcacg gtgaccatca ccaacacggg atcagcgccg gcgggccaat ggcgcaccgg
     9121 tgcactcacg tggaggggcg atggatacga ggttcgcagc cccatcgccg tcaaggggac
     9181 gaccttgtcg gctccgacga gcgtgtccgg aactggcacc tccggatcgg cgtcgctggc
     9241 tctgcaattc ggcttcgcag gcccttacac cgcaaccgcc gaagggctgc tggcggcaac
     9301 gcacactccc ggaaccatcg ctcaggaccc ggacacgacc taccccagcc ctgacgacgg
     9361 cgctggcgtg gtgtctgtcc cgttcaccct gagcggggtc ggagtggcca aatgggcact
     9421 gaacatgccc gatccgaccg acctggacat ctatctgttc ggtccaggcg gcaacctcgt
     9481 cggtgcgagc acgaacggtg ggacgaagga gtcgatcttg ctcaacagcc ccgcggatgg
     9541 cacctacacg atggtcatcc acggttgggc cgtgggtgca accccgctgc agttcgacct
     9601 gcgtagttgg ctcgttccgg cagaggacgg tgctggaagt ctgtcgatca gcagcggagc
     9661 cagcacaacc gcgaccatcg gcggaagcca caccgtgggg atcgcctgga ccggcctcac
     9721 tgctggcgag gaatacttcg gcagcgtgac gcaccgcaac ggcacggcgg tcgtcggccg
     9781 gaccgtggtg tccgtaaccg gttagccaac ctccgacagc gatcgggctc gggtccggcg
     9841 tcgatgactc ctcgtcctcg gcgttgggcc tgagcccgtc gcgctcggcc cgagcgcgac
     9901 ggtcactcgg tcactcggtc actcggtcac acggtcgcgc ggcgacacgc ggcatacgag
     9961 ctgatgctag cgagcacgac gaatgccgca ccgatgatga ccgtggccca cgctccccaa
    10021 ttggccaaca gcggcgcgac aacgctccac ttccacacca cgagtgcgac ggccgcaaac
    10081 aggacgagca ggacaccgat gctgagcacg gcggtcgcct tggtgccgaa ggcccgcagc
    10141 acggtgccga acagggcgcc gacggtcagc gacagcagtg ccaggacgaa ggtgaccgag
    10201 aacgtctgcg cgatgttgcc gttgccgagc acggccacat cgaaggcata ggcgccgacg
    10261 aaccagtggc cagtgagccg ctccagaccg agcatcagca ccgccgcggc cgcagtgacg
    10321 gcggcagtga cgacgaaccc ggccgtcgtg cccagccaga agtgccgccg ggtgctgccg
    10381 aaggccagcg ccatcgcgaa gttgcgggtg gccgccaaca cccccaccga gacgaggaag
    10441 cccggcaagg acaggatcgc gcccgagttg gcccggaacc cctcctcgaa atccttcgac
    10501 acggggaagc ccgtcgcgat gcccaccacg atgacgatga ggatgctgag cagcagcatg
    10561 aggcccaacg tgtagatcgg gacagccagc acgtattggc ggttggcgag atagagccgg
    10621 aaggagcggc gggagtaggt gatcacttga gtgacctcga ttcggacgcg gactgactgg
    10681 cggggagggg ggccgggtgg ggcgcgaggt gaggagcagg ctggtcggac tcgccgtgct
    10741 gaccgatcgc cgccacgaac tcctgcaggg tggtgggctc gatcgacacc cgcgccgcct
    10801 cggcgcgctg ccgttcgtcg gagctcagct cgccgcagat ggtcgccgtg acgatgccgc
    10861 cgagccgcct cgtcgagagc agctcacgac cagccgcgag cgcgaggaca tcggcctcga
    10921 acccgcgcgc caggaaggcg gtgcgccggg cgtcctcgac atcggcctgc aggacgatcc
    10981 gcccacggtc gaggacgagc acctcctcga tcagctcggc cgcctcgtcg atgagatggg
    11041 tggacatgat gaacgtgcgc ggcgcagtgg tgccgtcagc cgcccgctcg tggctcgcgg
    11101 catacacggc cagcagttcg tcgtagaaga tgccgcgcgc ggtcgcgtcc agtccgaggt
    11161 agggctcgtc caggaaggtg tatgccgctc cgctggcgag cgcgaggacg atcgccaagg
    11221 cggagcgctg gccgcgggag agcttgcgcg atcgggtgcg gcgcgggatc cggaagcgtc
    11281 gcatcaggcg ggtcgcggtc tcgtcgtccc actcggggta gaaggtgggc atgaccgcga
    11341 ggatgtcggc gaccgagaag gcgtcgttgt aggcctggtc ttcgtgcacg aagcacagcc
    11401 gggcgagggt cggagcgtgc tccagcgggt cgtggccgtc gatgcggatc tctccgccgt
    11461 cgcggaaggt gtgcccggcg atgcacgcga gcagcgtggt cttgcccgtg ccgttggcgc
    11521 cgagcagacc gtagatccgg ccgggctcca gggtggccga gacgtcgacg agcacctccg
    11581 tcgtgccgta gctcttggtc agaccgcgca gggtgatccc ggcacttgcc gtggtgattc
    11641 tggtggcgat gccggtgggg gtgcctgcgg tcgcggtgcc ggtcgcgggg ccggcggtgg
    11701 gggtgctcat caacgctcct tggtgacgag ggcgacgatc tcatcgatcg acaacccgag
    11761 ccggcggcct tcttcgacga agggccggac gaactgctcg gagaaggcgt cgcggcgctg
    11821 agcgcgcaaa gcctcggggg cgccggcggc aacgaacatg cccagcccgc gtcgtttgta
    11881 gacgattccc tggtcgacga gccggttgag ggccttgccg gcagtggccg ggttgatccg
    11941 gtaatgcgcg gccaactcgt tggtggatgg cacctgcgtc tcctcgggga tgagcccggc
    12001 caggatgtcg gcggcgatcc ggtcggcgag gtccatgaag atcggccgcc cgtcggtcca
    12061 gtccgtcatc ccgcctcctt cagtggttcc ttcagtggtt cgttactcca ctaactaacc
    12121 atggtggcgg cggggtgtca agcgggtcgc tcgcgcggcg tgggagagtt cgttcatgag
    12181 tccggtcgac atcgaggagg ccgtgcgcag gtatgccgcg agctggccct cgctggtccc
    12241 tgtcgccgac gccttcgtca ccacggtgac gggcatcctc gacgacgcgg gcatcaacta
    12301 tctgaccgtc accggccggg ccaaggccgt cgcgtcgttc gccgccaagg ccgcccgcga
    12361 gcgcgacggc gcccgggcgc atcccgaccc gatgcgggac atcaccgacc aggtcggagt
    12421 tcgggtcatc acctacgtcc gcagtgacgt cgacgccgtc gccgagttgc tggccgacca
    12481 actgacggtg ctcgacgatc gcgacatggg ccgcgagacc gcgcgggccg gccggttcgg
    12541 ttacgcgagc aggcacctgc tggtgtcgct cgactcaggt gagcacacgg catacgagcc
    12601 gacgagatgc gcgtcgatcc agttgcggac cgttctgcaa catgcctggg ccgagttcga
//
LOCUS       DAOMZY010000056        12233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_4835_length_12233_cov_6.002299, whole genome shotgun sequence.
ACCESSION   DAOMZY010000056 DAOMZY010000000
VERSION     DAOMZY010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 12233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12233
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4835_length_12233_cov_6.002299"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..471
                     /locus_tag="PLK64_04845"
     CDS             118..471
                     /locus_tag="PLK64_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HPV79103.1"
                     /translation="MRLAVARIDPWSAMKMSFLLSFAAGVGLVVATMVLWMILSGMNV
                     FNDIDGLVRSLQPNSADPFSIMDWIGFPRVTSLSMVIAVIDVILLTALGTLGAYLYNI
                     CASLVGGVQLTLTDD"
     gene            641..717
                     /locus_tag="PLK64_04850"
     tRNA            641..717
                     /locus_tag="PLK64_04850"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:675..677,aa:Ile,seq:gat)
     gene            747..893
                     /locus_tag="PLK64_04855"
     CDS             747..893
                     /locus_tag="PLK64_04855"
                     /inference="COORDINATES: protein motif:HMM:NF038356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DLW-39 family protein"
                     /protein_id="HPV79104.1"
                     /translation="MPKKIWVALAAAALAALAMRKSVADQQAERDLWAEATDDVRSSD
                     EPGR"
     gene            1111..1183
                     /locus_tag="PLK64_04860"
     tRNA            1111..1183
                     /locus_tag="PLK64_04860"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1144..1146,aa:Ala,seq:tgc)
     gene            1453..2706
                     /locus_tag="PLK64_04865"
     CDS             1453..2706
                     /locus_tag="PLK64_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HPV79105.1"
                     /translation="MIEYRPRIADGELAERMESAGAVLIEGPKSCGKTATASRRASTV
                     FRMDTDDSARDLVDTAPELLLGAAQPVLFDEWQVAPKLWNLVRRQVDDLGGEPGRFLL
                     TGSATPRDDATRHSGAGRYSILRMRPMSLFETGISTGAVSLAGLFDGDFTPSMAPNVT
                     VPNLVDCIVTGGWPALLDVPVRVAQRWVVDYLSSLVEVDLPQMGVRRDPATLRRFLAS
                     LARGTGTELSTQAIATDVAGPDRTLDRDTAAGYLDILARLMVTEDVPAWAPHMRSATP
                     LRKSAARFMVDPSLAVAALGVGPEQLLRDLNATGFLFESLVLRDLRVYSQPLRGRLAR
                     WRDNNNHEVDIIITLDDGRWGALEVKMNPAAADGAAASLLRFKDKVDLDKVGEPAFLG
                     VVTTRTAAVRRQDGVHILPIAALGP"
     assembly_gap    2787..2886
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2905..3789)
                     /locus_tag="PLK64_04870"
     CDS             complement(2905..3789)
                     /locus_tag="PLK64_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPV79106.1"
                     /translation="MGTAAGRQPSSEDERLSTPGADGVSREALLYRPRGGTSRGGLLW
                     IHGGGRVSGIPQFDDIQCRRLADDAGVTVLSVNYRLAPEHPFPAALDDCATALSWFTA
                     WCAANDIPGPLAIGGASAGGGLAAELAQRATDEGIELAHQVLVYPMLDDRSLGRGATN
                     RGRLVWTPTSNRFAWSAYLGHPAGASEQRPYAVAARREDLRGLPSAWIGVGDLDLFHD
                     EDVHYARRLRDAGVRVHLRVEPGMYHGADAVPGVKDSDRIRAFHADMSSSLRHALDLV
                     ESSHEPRDRSRDEATDGA"
     gene            complement(3982..6006)
                     /locus_tag="PLK64_04875"
     CDS             complement(3982..6006)
                     /locus_tag="PLK64_04875"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HPV79107.1"
                     /translation="MTNSASKATPTSASALSVRASAAHRPVSPLLFGLFYEDINLSSD
                     GGLNANLVNNHSFEGVYLDPKHKELRALAMRTPTTKHLDRARHWETSGPAAKLSVLGT
                     DAVASHGHYGRLTGGSGATLTNHGYPGDAGPSVAVRAGVNLRFTALARGPQGGSRCHV
                     VLLAGDGNLVAEADFTVPAGDQWTQVVAELSPTRTVRASLQLVLPDGGTLDLDEVRLI
                     PEDHWGAGDPRWSQGLFRRDLVEALRDLAPRFMRFPGGCIVEGLTLENAYDWKASVGP
                     LAERRPNYNLWGMHRPDGDYSQSLQIGFYEYFLLCEDLGMEPMPVVSAGLACQFRSRE
                     VTPVDGADFSKVVQDTLDLIDWATGDPATNAWAAKRAAGGHPEPFRLNLLGIGNENHG
                     PVYLERFERIRAAVDAHHPGLTIIMSSGTAPAGKAFDQSWAHARTCGDAKTVVDEHFY
                     KSPAWFIDQVNRYDDYPRGGAQVFMGEYAAHVPTLPIPRRLRLPANTIKSAIAEAAFL
                     TGVERNADVVAMTSYAPLLNHLESELWQHNLIDFDGFTVTPTANAAVQKLFATTLGER
                     IVDLDGELPAGVFASASRSDDRLCVHLVNTTTIPQDVTLTVDLPLHGAGSATVLAAPH
                     GIQPSRRMGSRERAKLLEDHLALAVHDGAVSLTLRPQSVSALVFGLASVS"
     gene            complement(6018..7538)
                     /gene="araA"
                     /locus_tag="PLK64_04880"
     CDS             complement(6018..7538)
                     /gene="araA"
                     /locus_tag="PLK64_04880"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HPV79108.1"
                     /translation="MSTPLASRPLAVHFLTGSQEMYGEETLRQVAEQSRRVADELGAH
                     GLGVELQWQPVLTDSTSVHRAVLAANADEQCAGVITWMHTFSPAKMWIAGLDALRKPL
                     LHLHTQADARLPWGSIDMDFMNLNQAAHGDRELGHVVGRLGLARAIVSGHVSNPIVRQ
                     RVSEWASAARGAHELRTMRVARFGDNMREVAVTDGDKVEAQIRFGVSVNSYGVTELVN
                     LVAAAPEAALNELVTEYLDTYAVAPDLRPGAARHTSLRYGAAIEWGLRTFLADGGFTA
                     FTTNFEDLGGLRQLPGLAVQRLMADGYGFGGEGDWKTSVLLRTLKAMGAGRTGGTSFM
                     EDYTYDLSTPGEERILGAHMLEVCPSIAGTTPTLEIHPLSIGGKEDPVRLRFTAAPAP
                     GVVVGLSDLGDRFRLTLNEIEVVDPTEPLPRLPVASATWRPEPDFATSTESWILAGAP
                     HHTVLSTAVGTPELRHLAEMLRTELVLIDRHASVEGIRDRLRWNAAYHRLNNHLCV"
     gene            complement(7580..8257)
                     /locus_tag="PLK64_04885"
     CDS             complement(7580..8257)
                     /locus_tag="PLK64_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HPV79109.1"
                     /translation="MGVAPELQQVVDMLRDQLSELHAELVCNHLVVWTAGNIAARVPG
                     HDLMVIKPSGVPYDELTPDAMVVCDLNGKVVEGERAPSSDSAAAAYIFRTMPHVGGVV
                     HTHSTYATAWAARGEAIPCVLTMMADEFGGEIPIGPFALIGDDSIGRGVVETLRASRS
                     TAVLMRQHGVFSVGRDARAAVKSAVMCEDVARTVHIARQLGQPIALQQNDIDSLYDRY
                     QNVYGQR"
     gene            complement(8259..9278)
                     /locus_tag="PLK64_04890"
     CDS             complement(8259..9278)
                     /locus_tag="PLK64_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPV79110.1"
                     /translation="MASAHPKTPVMADVALLAGVSHQTVSRVLNGHPHIRQSTRERVE
                     RAIAQLSYQPNTAARALVRGRSGLIGIVTGSGAFYGPRSAHQGVSAAARKAGYSVTSV
                     ELDDITEATLAAAVEDLSRLGVEGLIVVAGHDAAVDVARKRAGALPIVVVEGDLTRAA
                     MSAGVDQVAGAVAATEHLIGLGHRRIAHISGPLHWCEARARLEGWQLAMGRSGLPAVH
                     LLRGDWSPESGYTAGQQIAADAEITAVFASNDQMALGALRALREAGRSVPGDVSLVGF
                     DDIPEAAYLFPPLTTVHQDFAAVGHQAIAVLAAALSGAELPTPGLLSAPLIERASTAP
                     PRKDR"
     gene            9389..10981
                     /locus_tag="PLK64_04895"
     CDS             9389..10981
                     /locus_tag="PLK64_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013350653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPV79111.1"
                     /translation="MTATAGVVSAGRAVLGIELGSTRIKAVLTDPSGQRLASGVHSWE
                     SRLEDGHWTYSQEHIEGGLVACVADLRRDLHEVHGISLSTVAALGVSAMMHGYIALDE
                     DDQWLVPFRTWRDTTTAQAAQELGCALGVNIPQRWSVAHYYQAHLDGESHLARVVRLT
                     TLAGWVHLRLTGEHILGVGDASGMFPIDTATGTYDQWMLGVVDDLMERHGTTGPSRPL
                     EHLLPRVLRAGRPAGTLTPAGARLLDPSGGFDPGALVCPPEGDAGTGMVATNSVRPRT
                     ANVSVGTSVFAMTVLDDVWRSADPNVDVVCTPEGAQVAMVHANNGAHELDAWAGVFAD
                     FARKLSLDVTRDDVFRVLLDAADEGANNAGGTVVHNFIAGEPVVGVEAGRPLMARGQS
                     DRLTLADLVRANVYTVFASLAIGMRSLRIAGVTTDNVVAHGGVFRTKGVAQQALAAAL
                     DSPVSVLRGAGEGGAWGMALLAALLLHPDLDLPDFLTQVVFASTAAETVVPNPEDVAG
                     FAEYLSRFEGLLPLQAMAAQLP"
     gene            complement(11196..>12233)
                     /locus_tag="PLK64_04900"
     CDS             complement(11196..>12233)
                     /locus_tag="PLK64_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPV79112.1"
                     /translation="LAALDSTARQLPNPKLLRRPSLRREAQSTSALEGTYEPLQAVLT
                     ADAEGPTSLTMREVLNFVTMADVAFDWVAERRRLSASMLSELQRTLVRGTPAEGPNSG
                     DIRKIQVVIGQRRTASVGELPVRAARFVPSPPGADLEASVRDLVTWINEDHRATIDPV
                     VGAALAHYQFETLHPFHDGNGRIGRLMIVIQLYAAGVLGEPTLTLSPWFEARRTEYYD
                     RLFAVSTQGEWNHWVSFFAQGLEESAVRTRQQMLDLVAVQSTLKEVVRASSLRADSAH
                     KLVDYAVANTVFTVRQVERDLVLSYGRANGLVRDLVQLGILSESGDAYRRRFFAPTVL
                     DVLLSAEPGRG"
BASE COUNT         1926 a         3913 c         4207 g         2087 t
ORIGIN      
        1 ggaccggccg ctggaccggc cgctggaccg gccgctggac cggccgccaa gcccgcggct
       61 agggccgggg ctaccactac tgccccgcga acggctcccc gaccgaccgg ccgacgcgtg
      121 cgtctcgccg tggcacggat cgacccgtgg tcggcgatga agatgtcgtt cctgctgtcc
      181 ttcgcggccg gtgtcgggct cgtggtggcc acgatggtcc tgtggatgat cctctcgggg
      241 atgaacgtct tcaacgacat cgacggcctg gtccgcagcc tgcagcccaa ctcggcggac
      301 ccgttctcga tcatggactg gatcggcttc cctcgagtga cctcgctgtc catggtgatc
      361 gcggtcatcg acgtcatcct gctcacggcg ctcggcacgc tcggggccta cctctacaac
      421 atctgcgcct cgctcgtcgg gggcgtgcag ttgacgctga ccgacgactg acgagcctct
      481 cggacgccgg cctgctcggt ggcagccgtg gcctctccac gcgcgatctg gggttacgcg
      541 cggtggtgag cgggcggcat acatgggccg ttttgatcga ggtcgcggct gtggggtagt
      601 ctggcgcgtc gcggcggtgc tccgccgcac gatggtgcaa gggcctatag ctcagacggt
      661 tagagcgctt ccctgataag gaagaggcca caggttcaag tcctgttagg cccaccaccg
      721 tcgttctcca agccgggagg tatgccgtgc ccaagaagat ctgggtagcg ctcgcggccg
      781 cggcgctcgc cgccttggcg atgcgcaagt ccgtggccga tcagcaggcc gagcgtgact
      841 tgtgggccga ggcgaccgac gacgtccggt cgagcgacga gccgggccgc tgaccgtccc
      901 ggctgtttcg gggccatggc gcaattggta gcgcacctgc tgtgcaagca gggggcccac
      961 gcagcggcga gttgtggcgg cttgaccgcc tggcccagga cgggctagag acgggcgaga
     1021 ccaccttcga ggtcgggtat cctcttggag tccctccggg agcttcgtcg ctctcagcgg
     1081 ctcgtcctta gtcggcgaag tcgttcacca ggggaagtgg cgcaattggt agcgcaccgc
     1141 ttttgcaagg cgggggttgc gggttcgagt cccgtcttct ccacttcagt cgcaggtctc
     1201 ggcagtttcg acggccaggt tgacctcgcg cttgaccgaa cgttggccga ggacgctccg
     1261 gcatcctgac cattgaggtc gaatccaacc agcgctccag atggacacca tcggcgtgaa
     1321 gctccgcagc agctgcgttc acgtcgacgt tggccggggc cggggcagtc gtggccgtag
     1381 gtataggatt tgcggaatat tgagtatagg attcacgggt tagtgggtat cggattgacc
     1441 gaggaggcca ccgtgatcga gtatcggccc cgcattgcgg atggcgaact ggctgaacgc
     1501 atggagtccg cgggagccgt cctgatcgag ggtccgaagt cgtgcggcaa gacggccacg
     1561 gcgtcgcgtc gtgcctcgac cgtgttccgg atggacaccg acgacagcgc ccgtgatctg
     1621 gtggacaccg ctcccgaact gctcctgggt gccgctcagc cggtgctgtt cgatgagtgg
     1681 caggtggccc cgaagctctg gaacctcgtg cgtcgacagg tcgatgacct cggaggtgaa
     1741 ccggggcggt tcctcctgac ggggtcggcc accccccgag atgacgcgac tcggcacagt
     1801 ggcgcgggac ggtactcgat ccttcggatg cgtccgatga gcttgttcga gactggcatc
     1861 tcgacgggtg cagtctcgct ggcaggtctc tttgacgggg acttcactcc gtcgatggcc
     1921 cctaacgtga cggtgccaaa cctggtggac tgcatcgtca cgggcggatg gcccgctctc
     1981 ttggacgtgc cggtgagggt ggcccagcgg tgggtggtgg actacctgag ttccttggtt
     2041 gaggtggacc tgccccagat gggggtccga cgagaccccg ccacgctccg ccgcttcctc
     2101 gcgtccttgg cccgtggcac cggcaccgag ctcagcaccc aggccatcgc gaccgacgtt
     2161 gccggacccg accggacgtt ggatcgggac acggccgctg gctatctgga cattctggcc
     2221 cgtctcatgg tgaccgagga tgtccccgcc tgggcccctc acatgcgctc ggcgaccccg
     2281 ttgcgcaagt cagcggcgcg tttcatggtc gacccatcac tggccgtcgc cgcactcggt
     2341 gtcggcccgg agcagttgct ccgtgacctg aacgcgaccg gattcctctt cgagtccttg
     2401 gtgttgcgcg acctgcgcgt ctactcgcaa ccgctccgcg gccgcttggc ccgctggcga
     2461 gacaacaaca accacgaggt cgacatcatc atcaccctcg acgacggacg gtggggcgca
     2521 cttgaggtga agatgaaccc cgcagctgcc gacggtgcag cagcctccct gctgaggttc
     2581 aaggacaagg tcgacctcga caaagtgggc gaaccagcgt tcctcggtgt cgtcaccacc
     2641 cggacggcag cggtgcgtcg ccaggacggc gtccacatcc tccccatcgc cgcactcggc
     2701 ccctaacggt tcagcacgtc accgctcggc ggttgagggc gctcggccgt caggccccat
     2761 cggtcgcctc atcccggctc ctgtcannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnngttg agggcgctcg gccgtcaggc cccatcggtc gcctcatccc ggctcctgtc
     2941 acggggctcg tggctcgact cgaccagatc aagcgcgtgc cgaagagaac ttgacatgtc
     3001 tgcgtgaaaa gcgcggatcc gatcggagtc cttgactccg ggcacggcgt cggcgccgtg
     3061 gtacatgccg ggctcgacgc gcagatggac ccggactccc gcgtcgcgga ggcgccgcgc
     3121 gtagtggaca tcctcgtcgt ggaacagatc gaggtccccc actccgatcc acgccgaggg
     3181 caaaccgcgc agatcctccc ggcgggcggc cacggcatac ggacgttgtt cgctcgcccc
     3241 cgccggatgc ccgaggtagg ccgaccacgc gaaccggttg gaagtcgggg tccagacgag
     3301 ccgtccacgg ttcgtggctc cacgcccaag gcttcggtcg tcgagcatcg ggtagacgag
     3361 gacctgatgg gcgagttcga tgccttcatc ggtcgcccgc tgcgccagct ccgccgccag
     3421 tccgcccccc gcgctggctc cgccgatggc cagggggccg gggatgtcgt tggcggcgca
     3481 ccaagcggtg aaccacgaca aggcggtcgc acagtcgtcc agggcagcgg gaaacggatg
     3541 ctcgggcgcc aaccggtagt tcacgctgag caccgtcacc ccggcgtcgt ccgcgagtcg
     3601 acggcactgg atgtcgtcga actgcggtat gccggagact cggccgccgc cgtgaatcca
     3661 gagcaggcca ccgcgactgg tcccgccacg gggtcggtag agcaacgcct cacgggacac
     3721 accgtcggcg cctggggtgc tcagccgttc gtcttcggag gatggttgtc gccccgctgc
     3781 ggtgcccatc gcccgcacta agggaagggt ccctcgggtg gctggcagag ccagcatgac
     3841 gacagggctg cggaactcac gcgcgacatg gcgaaggtgt cgtgcgcgcc aggctacggc
     3901 ggccgtggcc gcggccagcg cgatggcagg ggtctgaggt gttcgcatcg ggtaggtgac
     3961 ctccgtgtta ggtcgtccag gtcaactgac cgaagccagt ccgaacacaa gtgcgctgac
     4021 actctggggg cggagggtga ggctgaccgc gccatcgtgg acggcgaggg ccaggtggtc
     4081 ctcgaggagc tttgcccgct cgcgagagcc catgcgccgg gatggctgta tgccgtgtgg
     4141 tgctgcgagg acagtggccg atcccgctcc atgcagcggc aggtcgacgg tgagggtcac
     4201 gtcctgcggg atcgtcgtcg tgttgacgag atggacgcaa agtcggtcat cggaacgact
     4261 ggcggacgcg aagacacccg cgggcaactc gccgtcgaga tcgacgatgc gctcgccgag
     4321 ggtggtcgcg aacagcttct ggacggctgc attggcggtg ggagtgaccg tgaacccgtc
     4381 gaagtcgatg aggttgtgct gccagagctc ggactccaga tggttgagca gcggcgcata
     4441 cgaggtcatc gcgacgacat cggcgttgcg ctcgacaccg gtgaggaacg ctgcctccgc
     4501 gatggcgctc ttgatcgtgt tggcggggag tcggaggcgg cgggggatgg ggagcgtggg
     4561 tacgtgtgcg gcatactccc ccatgaagac ctgagcaccg ccgcgcgggt aatcgtcgta
     4621 gcggttgact tggtcgatga accaggcggg gcttttatag aagtgctcat cgacaacggt
     4681 tttcgcgtcg ccgcaggtcc gcgcatgtgc ccagctctgg tcgaaggctt tgccggcggg
     4741 ggcggtaccg ctggacatga tgatggtcag gccggggtgg tgggcgtcga cggcggcccg
     4801 gatgcgttcg aacctttcca ggtagaccgg cccgtggttc tcgttgccga tccccaagag
     4861 gttgagccgg aaaggttcgg ggtgtccccc tgcggcccgc ttcgctgccc acgcattggt
     4921 cgccggatca ccggtggccc agtcgatgag gtccagggtg tcttggacga ccttcgagaa
     4981 atccgcgccg tccaccggcg tgacttctcg ggagcggaac tggcaggcca gtcccgccga
     5041 caccaccggc attggttcca tcccgaggtc ctcgcagagg aggaagtatt cgtagaagcc
     5101 gatctgcagg gactggctgt agtcaccgtc ggggcggtgc atgccccaca ggttgtagtt
     5161 gggtcgacgc tcggccagtg gaccgaccga ggccttccag tcgtaggcgt tctcaagcgt
     5221 gaggccttcg acgatgcagc cgccggggaa gcgcatgaac cgcggagcga ggtcccgcag
     5281 cgcctcgacc agatcgcgcc ggaacagtcc ctggctccag cgggggtcac cggcacccca
     5341 gtggtcctca gggatcaggc gcacctcgtc gaggtcgagt gtgcccccat cgggcaggac
     5401 gagctgcagg gaggcacgca cggttcgggt gggcgacagt tcggcaacga cctgggtcca
     5461 ctggtcaccg gctggcaccg tgaaatcagc ctcagccaca aggttgccgt ccccggccag
     5521 caagaccacg tggcatcgtg aaccgccctg cgggccccgc gcgagggccg tgaaccgcag
     5581 gttcaccccc gcccgcacgg ccacggaagg ccccgcgtcg ccggggtatc cgtggttggt
     5641 cagcgtggct ccagatccgc cggtgaggcg tccgtagtgt ccgtgggagg ccaccgcatc
     5701 ggtgccgagc acgctcagct tggccgcggg tccggaggtc tcccagtgcc gagcacgatc
     5761 gagatgcttg gtcgtggggg tcctcatcgc gagagcgcgc aattccttgt gcttggggtc
     5821 gagataaacc ccttcgaagc tgtgattgtt gacgaggttg gcgttgagtc ctccgtcgct
     5881 ggagaggttg atgtcctcat agaagaggcc gaagagcagg ggactgaccg gccggtgggc
     5941 tgccgaggcg cggacgctga gggcggatgc agaggtgggc gtggctttgc tggcgctgtt
     6001 ggtcactggg ttgctcctca gacacagagg tggttgttca ggcggtggta tgcggcgttc
     6061 cagcgcagcc gatcccggat gccctcgacc gaggcatgac ggtcgatcag gaccagttcg
     6121 gtgcgcagca tctccgccag gtgccgcagt tccggtgtgc caactgcggt gctcagcacg
     6181 gtgtggtgtg gggcgccggc aagaatccag gactccgtcg aggtcgcgaa gtcaggttcg
     6241 ggacgccacg tggcggacgc gacgggcaga cgcggcaaag gttcggtggg atcaacgacc
     6301 tcgatctcgt tgagggtgag cctgaaccgg tcccccaagt cactcaggcc gaccacgaca
     6361 cccggagccg gagccgcggt gaagcgaagc cggactgggt cctccttgcc gccgatcgac
     6421 agagggtgga tctccagcgt cggtgtggtg ccggcaatgc tcgggcagac ctcgagcatg
     6481 tgcgcgccaa ggatccgctc ctcccccgga gtgctgaggt cgtaggtgta gtcctccatg
     6541 aacgaggtgc caccggttcg acccgcgccc attgccttga gcgtgcgtag caggaccgac
     6601 gtcttccagt ccccctcgcc cccgaagcca tagccgtcgg ccatcaatcg ctgcaccgcc
     6661 aggcccggga gctgcctgag tcccccgagg tcctcgaagt tggtggtgaa ggccgtgaac
     6721 ccgccgtccg cgaggaaggt gcgaagcccc cactcgatcg cggcgccata ccgcaaggac
     6781 gtatgccgtg cggcccccgg ccgcaggtcg ggtgccacgg catacgtgtc gaggtattca
     6841 gtgacgagct cgttgagcgc cgcctccgga gcggcggcga caaggttgac cagttcggtg
     6901 acgccgtagc tgttgacgga gacgccgaag cggatctgtg cctcgacctt gtcgccgtcg
     6961 gtgaccgcga cctcacgcat gttgtcgccg aacctcgcga cgcgcatggt ccgcagttca
     7021 tgagcaccgc gcgctgcgct ggcccactcg gaaacccgtt gtcgcacaat cggattggac
     7081 acgtgaccgg agacgatcgc tcgcgcgaga ccgagtcggc cgacgacgtg gccgagttct
     7141 cggtcaccgt gtgcggcctg gttgaggttc atgaagtcca tgtcgatgga cccccatggc
     7201 aggcgcgcgt cggcctgggt gtgcaggtgc aggagcggct tgcgcagcgc gtcgagtccg
     7261 gcgatccaca tcttcgcggg cgagaacgtg tgcatccagg tgatgacgcc tgcgcactgc
     7321 tcgtcagcgt tcgccgcgag gacggcgcgg tgcactgagg tcgagtccgt cagcaccggc
     7381 tgccattgca gttcgacccc gaggccatgc gctccgagtt cgtcggcgac acggcgcgac
     7441 tgttcggcga cctggcgcag ggtctcctcg ccatacatct cctgactgcc agtgaggaag
     7501 tgcaccgcca acgggcggct ggcaaggggc gtggacattc tggtctcctc ggcgtgtttc
     7561 tgggtgttgc ggccgggctt caacgctgcc cgtagacgtt ttggtagcgg tcgtagagcg
     7621 agtcaatgtc gttctgctgc aaggcaatcg gttgcccgag ctgtcgcgcg atgtggacgg
     7681 ttcgagcgac gtcctcgcac atgaccgcgg acttaacggc tgcgcgagcg tcgcggccga
     7741 cgctgaacac gccgtgctgt cgcatgagca cggccgtgga ccgggacgcg cgcaaggtct
     7801 cgaccacgcc tcgaccgatg gagtcgtcac caatgagggc gaagggcccg atcgggatct
     7861 cgcccccgaa ctcgtcggcc atcatcgtga ggacacacgg gatcgcctcg ccgcgcgcag
     7921 cccacgccgt ggcataggtc gagtgagtgt gcacgacgcc cccgacgtgc ggcatcgttc
     7981 ggaagatgta tgccgcagcg gccgagtccg atgacggggc ccgttctccc tcgacgacct
     8041 tcccgtttag atcacagacg accatggcgt cgggcgtgag ttcgtcgtag ggcacgccgg
     8101 agggtttgat gaccatgagg tcgtgcccgg ggacgcgggc ggcaatgttg ccggcggtcc
     8161 acactacgag gtggttgcac acgagctcgg cgtgcagctc gctgagctga tcgcggagca
     8221 tgtctacgac ctgctggagt tcgggggcaa cgcccatctc aacgatcctt tcgcggcggt
     8281 gcggtgctgg cccgctcgat cagtggtgcg gagagcaacc cgggcgtggg caactcggct
     8341 ccgctcaacg ccgcagccag gaccgcgatg gcctggtgcc cgacggcagc aaagtcttgg
     8401 tgcacggtcg tgagtggtgg gaacaggtag gcagcctcgg ggatgtcgtc gaagccgacg
     8461 agggagacat cgccggggac gctgcggccg gcctcccgga gggctcgcag tgcgccaagg
     8521 gccatctggt cgttgctggc gaagaccgcc gtgatctcgg catcggctgc gatctgctgt
     8581 ccagcggtgt agccggattc ggggctccag tcgccccgaa ggaggtggac cgcgggcagg
     8641 ccgctgcgcc ccatggccaa ctgccacccc tccagccggg cccgggcctc gcaccagtgg
     8701 agcggaccgg aaatgtgcgc gattcgacgg tggcccaggc cgatcaggtg ctcggtcgcg
     8761 gccacggcac cggcgacctg atccacgccg gcactcatcg ccgcccgcgt caggtcacct
     8821 tcgacaacaa cgatgggaag ggcacctgct cgcttgcggg cgacatccac agcagcgtcg
     8881 tgccccgcca cgacgatcag cccttcgacg ccgaggcgag aaagatcctc cacagcggcg
     8941 gcgagcgtgg cttccgtgat gtcgtcgagc tcgacgctgg tcaccgagta gccggctttc
     9001 cgggctgccg ccgagactcc ttggtgagcg cttcgcggtc cgtagaaggc ccctgatccg
     9061 gtgactatgc cgatcagccc cgaccgccct cgcacgagcg cgcgcgctgc cgtgttcggt
     9121 tggtaactca gctgggcaat ggctcgttcg accctttcac gggtgctctg acggatgtgc
     9181 gggtgcccat tgagcaccct ggaaacggtc tggtgggaca cccctgcgag cagcgcgacg
     9241 tctgccatca ctggcgtctt gggatgtgcg ctggccattg ggcggtcctc cttgtccccc
     9301 caaattgtta gcgttaacat tcctccatgt caactgtgat ggtcagggat ctcgatcgca
     9361 tgtgcacgct cacacgtggg cgcgcgtcat gaccgcgacg gcgggggtcg tctcggctgg
     9421 gcgcgctgtg ctgggcatcg aactggggtc gacccggatc aaggcggtgc tcaccgaccc
     9481 gtcgggccag cgcctcgcct cgggggtgca ctcttgggag agtcgcctcg aggacggcca
     9541 ctggacctac tcccaggagc acatcgaagg ggggctcgtt gcgtgtgtgg ccgatctgcg
     9601 gcgcgacctc cacgaggtgc atggcatctc gctgagcacg gtggccgctc tcggggtgtc
     9661 ggccatgatg cacggttaca ttgctctgga cgaggacgac caatggctcg tcccattccg
     9721 gacctggcgg gataccacga ctgctcaagc tgctcaggag ctcggttgcg ccttgggcgt
     9781 caacatcccc caacggtggt ccgtggccca ctactaccag gcccatctcg acggtgaaag
     9841 ccacctcgcg cgagtggtgc gcctgaccac cctggccggt tgggttcacc tccgcctgac
     9901 cggcgagcac atcctcggag ttggcgatgc ctcgggcatg ttcccgatcg acacggccac
     9961 cggcacctac gaccagtgga tgctgggcgt ggtcgatgac ctgatggagc gccacggcac
    10021 cacaggtcct tctcgcccgc tggagcacct cctgccccga gtcctgcgcg cgggacgacc
    10081 agctgggacg ctcacacctg cgggagctcg gctgctcgac ccttccggag gcttcgaccc
    10141 cggcgcgctg gtgtgccctc ccgaggggga tgccggcacg gggatggtcg cgacgaacag
    10201 tgtgcggccg cgcacggcca atgtgagcgt cgggaccagc gtcttcgcca tgaccgtcct
    10261 cgacgacgtg tggcgcagcg cagacccgaa cgtcgacgtc gtgtgcactc ccgagggcgc
    10321 acaagtcgcc atggttcatg ccaacaatgg cgcgcacgaa ctcgatgcct gggccggtgt
    10381 attcgccgac ttcgcgcgca agctgtctct cgacgtgact cgcgatgacg tcttccgcgt
    10441 cctcctcgac gcagccgacg agggcgccaa caacgccggt ggaacggtgg tccacaactt
    10501 catcgccggc gaaccggttg tgggagtgga ggccggccgc ccactcatgg cccgcggtca
    10561 aagcgatcgg ttgaccttgg cggatctcgt ccgagccaac gtctacacgg tctttgcctc
    10621 gctcgccatc ggcatgcgat ccctccgaat cgcgggagtc accaccgaca acgtcgtcgc
    10681 gcacggcggc gtcttccgca ccaagggcgt ggcccagcag gcgttggcag ccgctctgga
    10741 cagtccggtg agcgtgttgc gcggggccgg agaaggaggg gcctggggca tggccctcct
    10801 cgccgccctc ttgctgcacc cagacctcga tctgcccgac ttcttgacgc aggttgtctt
    10861 tgcatcgacc gcagccgaga cggtcgtccc gaaccctgag gatgtcgccg ggttcgccga
    10921 gtacctcagc cgcttcgagg ggctccttcc cctccaagca atggctgcgc agttgccgta
    10981 gcggtcaggt tgcaggagag cggcgtaccc cgaaacagag ccagttctgg cacagctagc
    11041 ccgcgatcgt gctgtgctgg gcccacgcgg cggctggccg cgacgaagga gcgggcgggc
    11101 gccgtggggc gtccccttgg ctccaccact gactgactga ttgacacaag aagggcccga
    11161 ccgcctttgg tcgggccctt tcgcatccct ggggtttagc cccgacctgg ctcagcggac
    11221 aggaggacgt ccaagaccgt cggcgcgaag aacctccggc gataggcgtc gccagactca
    11281 gagaggatgc ccaactggac caggtctctg acaaggccgt tcgctcggcc atatgagaga
    11341 accaggtcac gctccacttg acgcacggtg aagaccgtgt ttgccacggc atagtcgacg
    11401 agcttgtgcg cgctgtctgc gcgaaggctc gatgccctga cgacttcctt aagggtcgac
    11461 tggaccgcaa cgaggtcaag catctgctgt cgagtccgca ccgccgattc ctcgagaccc
    11521 tgggcaaaga aggagaccca gtggttccat tcgccctgcg tactaacggc gaagagccgg
    11581 tcgtagtact cggttcgacg agcctcgaac cacggtgaga gcgtgagagt gggctccccg
    11641 aggacgccgg cggcatacag ctggatgacg atcatcagcc gtccgatccg gccgttgccg
    11701 tcgtggaagg ggtggagggt ctcgaactgg tagtgggcga gggcggcccc caccactggg
    11761 tcaatcgtgg cgcggtggtc ctcgttgatc cacgtgacga ggtcgcggac gctcgcctca
    11821 aggtcggccc caggtggcga gggcacgaac ctcgccgccc tgacgggaag ttcacccacg
    11881 gaagctgtcc gacgctgtcc gatcaccacc tgaatcttgc ggatgtcacc ggagttgggg
    11941 ccctccgctg gagtgccgcg gacgagcgtg cgctggagct ccgacaacat ggaagcagag
    12001 agccggcggc gctccgctac ccagtcgaac gcaacgtcag ccatggtcac aaagttgagg
    12061 acctcgcgca tcgtgaggct ggtggggccc tcggcgtcgg cggtcagaac ggcttgaagt
    12121 ggctcgtagg tcccctcaag agccgaggtg ctctgtgcct cccgacgcag cgaagggcgt
    12181 cgaagcagct tggggttggg gagctgtcgc gcagtgctgt ctagggctgc cag
//
LOCUS       DAOMZY010000057        12035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_4953_length_12035_cov_5.643239, whole genome shotgun sequence.
ACCESSION   DAOMZY010000057 DAOMZY010000000
VERSION     DAOMZY010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 12035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12035
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4953_length_12035_cov_5.643239"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..1053
                     /locus_tag="PLK64_04905"
     CDS             46..1053
                     /locus_tag="PLK64_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79113.1"
                     /translation="MTLDLQLLDPDDEQQLDAIVALFAATDAVDAPWRHGATPRSIRQ
                     EHLLGYDGDPEPFYLIRRGSDLVAVGAMALPSRDNLDSGWIYAAVHPDARRKGIGRWT
                     YTELIGLATDAGRTVLGTFGIDLPHTRGFVDAIGFEVRSRSVTRRVTFAELPAGATEA
                     ADAQALPYAADYDLLRVVGALPDDLMAGYVQAVAAINDAPTDDLEQQDEVFDAERVRA
                     YERSHAVRGETLYRVIARHRETGEFAGHTVVTVDPERPTFGDQHDTTVVDAHRGHRLG
                     VLLKTDMVRWLGEVEPACGMLTTSNAESNTHMIAVNDLLGYRPISHGIACQRTPSSPA
                     V"
     gene            1217..1354
                     /locus_tag="PLK64_04910"
     CDS             1217..1354
                     /locus_tag="PLK64_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79114.1"
                     /translation="MTAYVGRRRGANQAARMRDWTTILLGLLCALVVLSIPLGLLAAR
                     G"
     gene            1530..2267
                     /locus_tag="PLK64_04915"
     CDS             1530..2267
                     /locus_tag="PLK64_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPV79115.1"
                     /translation="MTTNPVKPQKLERPDGTPPRVLVVDDEHNLTELLSMALRYEGWE
                     VRSAGTGMAAVRAAREFEPDAVALDMMLPDMDGLEVLRRMREHNDRLPVLFLTARDAV
                     EDRIAGLTAGGDDYVTKPFSLEEVVARLRALMRRTVLVATEEDDSRLVVGDLTLDEDS
                     HEVTRAGAQIQLTATEFELLRYLMRNPKRVLSKAQILDRVWNYDFGGQANVVELYISY
                     LRKKIDAGREPMIHTMRGVGYVLKPAS"
     gene            2267..3832
                     /locus_tag="PLK64_04920"
     CDS             2267..3832
                     /locus_tag="PLK64_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPV79116.1"
                     /translation="MSEQPPAAAAGTAVAAAPAEFVAPAPTTAARSWHPSTWTLRSKL
                     VASMLVLFTVMTLVIAAASVLSLDRSLTAQLDDQLRSSLSKIPPQLGGTASSTSLRDG
                     DGDLGRGPGDEGFVVVVANGTPYTRQRDAITVAQIQLLQEAGLGNQPRNIDLGGELGE
                     YRVLAGTSARNSGAVVYVGLPRDRQVGTVGNAFRTALFASVLGLLAVGIGGAWLVTRN
                     LRPLTRVADTATRVSQLHLSSGQVALAERVPEEDTDPRTEVGRVGLALNELLDHVDSA
                     LNARHESEQRVRQFVANASHELRTPLASIRGYAELTRKEREPVPAGVVHAISRVESEA
                     KRMSTLVDDLLLLARLDAGRPLDQEDVDLTEIVVNAVSDAHAASPQHKWRLDLPDEPV
                     LVRGDSARLHQIVANLLGNARVHTLPGTTVLTSISREPGTVRLSVHDDGPGVPEAQQA
                     KVFQRFARGDSARSRTGGSTGLGLSIVAAVASAHGGSVQLTSTPGDTVFSVVLPAPDD
                     ASSTPRWAGRVTP"
     gene            3971..5920
                     /locus_tag="PLK64_04925"
     CDS             3971..5920
                     /locus_tag="PLK64_04925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018762401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPV79117.1"
                     /translation="MTELAMAPTSTTGPDQTTAATAATSSRDPSWVRPALLGLLAATA
                     VFYLWNLTSSGYANAFYSAAAQAGADSWKAFFFGSSDAANAITVDKPPASLWFMALSV
                     RIFGLSSFAILLPEVLMGVATVGVVYATVKRQFGAAAGLLAGGILALTPVAVLMFRFN
                     NPDALLVLAMALAAWATVRSIDESSPRWFALVGVFIGLAFLTKTLQAFLVVPGFALAY
                     LVAARPPLLRRIWHTVIGGLAIVASAGWWVAVVELLPASVRPYIGGSQTNSFLELTFG
                     YNGLGRINGEETGSVGNASARLTQIGLGRMFNSENGGQISWLLPAALILLLVGLAYRA
                     RMPRTDARRAAYLVWGSWLVITTLVFSLMAGIYHAYYTVALAPAIAALVAMGGAEAWE
                     RRDRWIGSVPLAAVTVITGVWSFVLLSRVTSWNGWLRVVVLALGLAAAAGFLLADRLP
                     AKAVPAVLGVALAAGLLGPAAYSVATVATPHTGSIVLAGPATSGGAGRAPGGMPPGGT
                     SRGGAGGGLLNASTPSAAVVQALSADASSYTWVAAAVGSQNAAGLQLGTQLPVMAIGG
                     FNGSDPSPTLAQFQEYVAAKQIHYFAAGGGFGRQNGGSRESSSIASWVSANFRAVTIG
                     GSTLYDLTQPASGGVGSTPAATRTA"
     gene            5938..6411
                     /locus_tag="PLK64_04930"
     CDS             5938..6411
                     /locus_tag="PLK64_04930"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HPV79118.1"
                     /translation="MATEPALAVAAPPAVTGDAQIGLASQLGRFAVVGAVSTVVHLGL
                     FALLHLAWPSQVANVAALVVATVVNTALNRRWTFRVTGGRAWRQHAQGFVVFLLTWGA
                     TSGGLALLDLGWPTAGTPIKLLALVAATGISTVVRFVAMRAWIFRIPAGDASSLG"
     gene            complement(6561..6650)
                     /locus_tag="PLK64_04935"
     tRNA            complement(6561..6650)
                     /locus_tag="PLK64_04935"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6614..6616),aa:Ser,seq:gct)
     gene            6825..8375
                     /locus_tag="PLK64_04940"
     CDS             6825..8375
                     /locus_tag="PLK64_04940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HPV79119.1"
                     /translation="MSDLDGYASPRGERSRRREIAERATRRSATLTLATTIFPGLGLL
                     RTRYKRLGVLVVIGVVLFAMAVIARVVMLGPTKAALSVAVNPNALLAAAAFALVAGLI
                     WVFTIILASLGTRDPSMTRRQRGGLSALTALLCMAVIVPMGTVVNYSLIQRDVVASLF
                     GVQQGTPEEEETNAQPGTGVDPWEGVSRVNMLLLGSDAGDDRIGTRTDSMIVASINPQ
                     TGDTLLFSLPRNLENVPFPKTNPLSKIYPQGYNCGPPDCLLNGVWTLAEANKKLFGSD
                     PNPGLTTVRGVIQEITGLRLHYTTVINLDGFESLVDAMGGVTVTINEKLPIGGHLNSA
                     GQLVGVDAWIQPGTQHLDGYHALWFARSRLASDDYNRMRRQRCLVGKILDQVNPVSML
                     QKYPALAQVAKDNITTNIAADDLPAWMELVRRIQGASIRSLTFTTQNINPGNPNFRLI
                     RTMIQDALHPAPATASASPTTSTKTTTGPTKSTSTKSTKTSTGPTKSTTTSSSITTDE
                     LVDLREAC"
     gene            complement(8435..8851)
                     /locus_tag="PLK64_04945"
     CDS             complement(8435..8851)
                     /locus_tag="PLK64_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79120.1"
                     /translation="MRRFLYAGAALLTAAALVAGPGGTAYAAPPVNGTTVVTGTPTAG
                     VPLAVQVTLYGVYPIVPYDFSLENRCWFGGRFAGPADSSETYPLLGPWYAAGSGATSS
                     ETVNLNDVPAGSVCKASISRGTSPVKGSITAYSVTD"
     gene            complement(8902..10047)
                     /locus_tag="PLK64_04950"
     CDS             complement(8902..10047)
                     /locus_tag="PLK64_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79121.1"
                     /translation="MRTVSTRHTRRYAARAAMLAILPLVCAGVLSGTPAAEAAAGCRI
                     YVASGDDYPAGNALNDNPSRFPEQLLADHLVAPGWCLYNQGKNGTTSSGYLSGGLAAS
                     AYNMRPDLHTITLGEQNSTIVNLIKSCFDKVKDHDFLGALTCAQTVQSNTTLWTSLTN
                     NYTTILSQDKIMASQRPGLVVAVTGYPNPYPRAADVVDEIAQLCVPLIDTALTCTVRW
                     AQLPAALAAIDSAFTKLNSTIKAALGPFQAGPNGWRYVFVDINPKFEDHCMTMKVAIK
                     TKVEHPEESGAVHQHDSPEVNFGCSESWFVEGSDGTAKPNYLLPATPGVLIEWSQTTK
                     GMGVYPDADGHQCIADAIWEADTIDPGTTPLKWKLGYGEASTTDYCQ"
     gene            complement(10085..>12035)
                     /locus_tag="PLK64_04955"
     CDS             complement(10085..>12035)
                     /locus_tag="PLK64_04955"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HPV79122.1"
                     /translation="DQLGGNGSFLCGYSDGVVTCTGATLDGSLDLIAGVPTERVVLIT
                     VLPPQEHGLVITNQAFVDPANTIAESNETNNYAVDTSIVISPFDVTLEKEGPTTAHQN
                     NTEDYTITVTNNGAAVTDVVVEDALPTGLIVLGATATPSNFTCQVFENPVNFVRCTGD
                     MGADGSATAVVTITVQVFVTKDGGTLDNEACVDPANTIVESIETNNCDTKSSVVDVFR
                     PNIAVHKSASVGSATIGQTFSYTVTASNIGDATAAAGWTVEDVLPSEVDFVSANGNNG
                     GTCVHDGSASGGTVTCTPTALAPGEQETYTIEVRANSTATTAFTNTASVSGAVDSDAA
                     AEPCISSTCGPETSVANNSDSVTVSAGGPSINLVVGDITDLPDPAEQGDAVTYTVVVT
                     NGGTQDALAADGHEVVVRVNVPTVGVTLDATVGTQGFLCVATAGDSIVTCTGDLNGGE
                     STTLSIRIITTSTTPPQLDVTVTADPANAITETSETDNVATEKTTVSSVACTGCIDLI
                     LGQISADPNPGVDGAQVTYEFAVTNIGDGTTASDLDPLVVAINLDTTFNESTFVSGVG
                     TNGFTCVANPAFPGDLAAPEVLCVASASGLTAGQGSLITIVTNVNTGGPSFVDFDVAI
                     DPGDVVGEDTNANNNGGLQIDVVAP"
BASE COUNT         1824 a         4271 c         3978 g         1962 t
ORIGIN      
        1 aaaaccaggt gacgcggccc gcgaccgagg gtcaccctgg aaggcatgac cctggacctg
       61 cagctgctcg acccggacga cgagcaacaa ctcgacgcga tcgtggcgct cttcgccgcc
      121 accgacgcgg tcgatgcgcc gtggcgccac ggcgcgaccc cgcgcagcat ccgccaggag
      181 cacctgctcg gctatgacgg tgacccggag ccgttctacc tcatccgccg cgggagcgat
      241 ctcgtcgccg tcggcgccat ggcactcccc agccgggaca acctcgacag cggctggatc
      301 tacgccgcag tccaccccga cgcccgccgc aagggcatcg gccgctggac ctacaccgag
      361 ctcatcgggc tcgcaaccga cgccggccgc accgtcctgg gcaccttcgg gatcgacctg
      421 ccacacacgc gcgggttcgt cgacgcgatc ggtttcgagg tgcgctccag gagcgtcacg
      481 cggcgggtca ccttcgccga gcttcccgcg ggggcgacgg aggcggcaga cgcccaggcc
      541 ctcccgtatg ccgcggacta cgacctcttg cgcgtcgtcg gagccctccc ggacgacctc
      601 atggccggct acgtgcaggc cgtcgcggcg atcaatgacg cgcccactga cgacctcgag
      661 cagcaggacg aggtcttcga cgccgagcgc gtgcgcgcct acgagcgaag ccacgccgtg
      721 cgcggcgaga ccctctatcg ggtcatcgcc agacatcgcg agaccgggga gttcgccggt
      781 cacaccgtcg tcaccgtcga tcccgaacgg ccgacgttcg gcgaccaaca cgacacgacc
      841 gttgtggacg cccaccgcgg gcaccgcctc ggagtgctcc tcaagaccga catggtgcgc
      901 tggttgggcg aggttgagcc ggcctgcggc atgctgacga cgagcaacgc cgagtccaac
      961 acccacatga tcgccgtcaa cgacctcctc ggttatcgcc cgatcagcca cgggatcgcc
     1021 tgccagcgca cgccgtccag ccccgccgtc tgagggctct gtccgaaggg cgccaacggc
     1081 ggtagaatct cctccgcggg cagggaaccc gcacggggta tgccgccgac gcaggtcggc
     1141 cacgccggtt ccgcgggggt cgcggggcag gcagcacaac tggggatcaa gcaaagtgtc
     1201 aagggggcta ggtgccatga cggcatatgt ggggagacgc cgcggagcca atcaggccgc
     1261 gcggatgcgt gactggacga cgatcctgct cgggcttttg tgtgccctcg tcgtgctgtc
     1321 aattccgttg ggactgttgg ccgctcgcgg ctgacctcaa cgtcggaccg atcaaggccc
     1381 tggggtgctg cgggctgccc acgctgccac gctgcactgt gtcgacgctg ttggcctcct
     1441 gtcgctgcgc cgtgagtgcg atgatcgctc acaggaagcg cacagcaaac acacacgagg
     1501 ccgacacgca ccgcggagag acttccacca tgaccacgaa ccccgtcaag ccgcagaagc
     1561 tcgagcgccc cgacggcacc ccgccgcgag tgctcgtcgt cgatgacgag cacaacctca
     1621 ccgagctgct gtcgatggcc ctgcgctacg agggctggga ggtgcgctcg gccggcaccg
     1681 gcatggcggc tgtccgagcc gcgcgcgagt tcgagcccga cgcggtcgcg ctggacatga
     1741 tgcttcccga catggacggc ctggaggtgc tgcgccggat gcgtgagcac aacgaccgcc
     1801 tcccggtgct cttcctcacc gcacgcgacg ccgtcgaaga ccgcatcgcc gggctcaccg
     1861 cgggcggcga cgactacgtc accaagccgt tcagcctcga ggaggtcgtc gctcgactgc
     1921 gcgcgctcat gcgtcggacc gtcctggtgg ccaccgagga ggacgactcg cgcctggtcg
     1981 tcggcgacct caccctcgac gaggactccc acgaggtcac ccgcgctgga gcccagatcc
     2041 agctgacggc aacggaattc gaattgctgc gctacctcat gcgcaacccc aagcgggtgc
     2101 tgtccaaggc ccagatcctc gaccgcgtct ggaactacga cttcggcggc caggccaacg
     2161 tcgtcgagct ctacatctcc tacctgcgca agaagatcga cgccggccgc gagccaatga
     2221 tccacaccat gcgtggcgtg ggctacgtcc tcaagccggc gagctgatgt ctgagcaacc
     2281 ccccgcagca gccgccggga cggccgtagc agccgcaccc gccgagttcg tcgcgcccgc
     2341 gccgaccacc gcggcccgct cgtggcaccc gtcgacctgg accctgcgca gcaagctcgt
     2401 cgcctcgatg ttggtgctct tcacggtgat gaccctcgtc atcgccgcgg catcggtgct
     2461 ctccctcgac cgttccctca ccgcccagtt ggacgatcaa ctgcgttcct cactcagcaa
     2521 gatcccgccc cagctcggag ggaccgcgtc gtcgacctcc ctgcgggacg gcgacgggga
     2581 cctcggccgc gggcccggcg atgagggctt cgtcgtggtc gtcgccaacg gcacccccta
     2641 cacccgccag cgcgacgcca tcacggtcgc ccagattcag ctgctgcagg aggccgggct
     2701 gggcaaccag ccccggaaca tcgatctcgg cggcgaactc ggcgaatacc gggtcctcgc
     2761 gggtacctcg gcccgcaaca gcggcgccgt cgtctacgtc ggcctccccc gggaccgcca
     2821 ggtcggcacc gtcggcaacg ccttccggac cgcgctgttc gcctcggtgc tcggcctgct
     2881 cgccgtcggc atcggcggcg cctggctggt cacgcgaaac ctgcgcccgc tgacccgggt
     2941 cgccgacacc gccacccgcg tctcgcagtt gcacctgtcg tccgggcagg tcgcgctcgc
     3001 cgaacgggtc cccgaggagg acaccgaccc gcgcacggag gtcggccggg tcggcctcgc
     3061 cctcaacgag ttgctcgacc acgtcgactc cgccctcaac gcgcgtcacg agagtgagca
     3121 gcgggtgcgc cagttcgtcg ccaacgcctc acatgagcta cggaccccgc tcgcctccat
     3181 cagagggtat gccgagttga cccggaagga gcgcgagccc gtccccgcgg gggtggtcca
     3241 cgcgatcagc cgggtcgagt ccgaggccaa gcggatgtcg acgctcgtcg acgacctcct
     3301 cctgctggcc cggctggacg ccggccgccc cctcgaccag gaagacgtcg acctcaccga
     3361 gatcgtcgtc aacgccgtca gcgacgccca cgccgcctcg ccgcagcaca agtggcgcct
     3421 ggacctgccc gacgagccgg tgcttgtgcg cggagacagc gcccgcctgc atcagatcgt
     3481 cgccaacctc ctgggcaacg cccgcgtgca caccctgccc ggcacgacgg tgctcacgag
     3541 catcagccgc gagccaggca ccgtgcggtt gtcagtacac gacgacgggc ctggggttcc
     3601 cgaggcccag caggccaagg tcttccaacg cttcgctcgc ggcgactcgg cccgctctcg
     3661 caccggcggg agcactggtt tgggcttgtc gatcgtggcc gctgttgcca gtgcccacgg
     3721 cgggtcggtg cagttgacga gcacgccggg cgacaccgtc ttctccgtgg tgctgcctgc
     3781 gcccgacgac gcctcctcga ccccgcgttg ggccggtcgg gtcaccccct gatcgatcca
     3841 ccaatccggg ccgacgcatc cggtgccacg tctccccccg cgcgaggtgt ggcaccggtg
     3901 gcctcgtcct cacggaccag tcctggcagc cacacaggta gcccatagcg gcgacgacca
     3961 ccgtggtcga atgaccgagc tcgccatggc ccccacctcg accaccgggc ccgaccagac
     4021 caccgccgcc actgcggcaa cctcctcacg tgacccgtcc tgggtccgcc ccgccctgct
     4081 cggcctcctc gcggcgaccg ccgtcttcta tctgtggaac ctcacctcca gcggttacgc
     4141 gaacgccttc tactccgccg cagcccaggc cggagccgac tcgtggaagg cgttcttctt
     4201 cggcagttcc gacgccgcca acgcgatcac cgtcgacaaa ccgcccgcct cgctgtggtt
     4261 catggccctg tcggtgcgga tcttcggcct cagctccttc gcgattctgc tgcctgaagt
     4321 gctcatgggg gtcgccactg tcggcgtcgt ctacgccacc gtgaagcgcc aattcggcgc
     4381 tgccgcaggg ctgctcgctg gggggatcct cgccctcacc ccggttgcgg tgttgatgtt
     4441 ccgcttcaac aaccccgacg cgctgctggt cttggccatg gcactagccg cgtgggcgac
     4501 ggtgcgttcg atcgatgagt cgtccccgag gtggttcgcc ctcgtcggcg tcttcatcgg
     4561 cctggccttc ctcaccaaga ccctccaagc cttcctcgtc gtccccggct tcgccctggc
     4621 ctacctcgtc gccgcccgac ccccgttgct gcgccggatc tggcacaccg tcatcggggg
     4681 cctcgcgatc gtcgcgtcag ctggctggtg ggtcgcggtc gtcgagctcc ttcccgcaag
     4741 cgtgcgcccc tacatcggtg gctcccagac caattcgttc ctcgagttga ccttcggcta
     4801 caacggactc ggtcggatca acggcgagga gaccggctcg gtcggcaacg cctcggcccg
     4861 cctgacccag atcggcctgg gccgcatgtt caactccgag aacggcggcc agatctcgtg
     4921 gctgctcccc gcggcgctca tcctcctgct cgtcgggctg gcctaccgcg cgcgtatgcc
     4981 gcgcaccgac gctcgccgcg cggcatacct cgtctggggc tcgtggctcg tcatcaccac
     5041 cctcgtcttc tcgctgatgg ccggcattta tcacgcctac tacaccgtcg cgctagctcc
     5101 agcgatcgcg gcccttgtgg cgatgggcgg cgccgaggcg tgggagcgcc gcgaccggtg
     5161 gatcggttcg gtgccgttgg ctgcggtcac ggtgatcacc ggcgtctgga gctttgtcct
     5221 gctctcgcgg gtcacttcgt ggaacggctg gcttcgtgtc gtcgtcctgg cgctcggcct
     5281 ggccgccgcc gcgggcttcc tgctcgcaga tcgactgccc gccaaggcag ttcccgccgt
     5341 gctgggagtg gccctcgcgg ccgggttgct cggccccgcg gcatacagcg tggcgaccgt
     5401 ggccactccc cacaccggct cgatcgtcct cgccggcccc gccacctccg gcggtgcggg
     5461 acgagcacca gggggtatgc cgcccggcgg cacctcccgg ggcggtgcgg gcggtgggct
     5521 gctcaacgcc tcgacgccct cggccgcggt cgtccaggct ctgtcggccg acgcgtcgtc
     5581 ctacacgtgg gtcgcagcgg ccgtcggctc acagaacgcc gcgggcctgc agttgggcac
     5641 ccaactcccg gtgatggcca tcggcggctt caacgggtcg gacccgagcc cgacgttggc
     5701 gcagttccag gagtatgtgg cggcgaaaca gatccactac ttcgcggcgg ggggcgggtt
     5761 cgggcggcag aacggcggca gccgcgaatc ctcgtcgatc gcctcgtggg tgtcggccaa
     5821 cttccgtgcg gtgacaatcg gcggctcgac gctctacgac ctcacccaac cggcctccgg
     5881 gggcgtcggc agcacgcctg ccgcgacccg gacggcctaa gggtcggcgt gcgcaccatg
     5941 gccaccgaac ccgccctggc agtggcggcc ccgcctgccg tgaccggaga cgcgcagatt
     6001 gggctcgcga gccaactggg ccgattcgcg gtggtcgggg cggtgagcac ggtcgtacac
     6061 ctgggcctgt tcgcgctgct gcacctcgcc tggccgagcc aagtcgccaa cgtcgccgcg
     6121 ctcgtcgtcg cgacggtggt caacacggcc ctcaaccggc ggtggacctt ccgcgtgacc
     6181 ggtggtcggg cgtggcgcca gcacgcgcag ggtttcgtgg tcttcctgct cacctgggga
     6241 gcgacgagcg gggggctggc gctcctcgac ctcgggtggc cgacggcagg gactcccatc
     6301 aagctgctgg ccctcgtcgc cgccaccggg atctcgacag tcgtgcggtt cgtcgccatg
     6361 cgtgcctgga tcttccgcat cccggcgggt gatgcgtcct cgcttggatg aggttggtgg
     6421 cacttgcatg aggctgtagc ggcatacaag cgtcactagc ctcatgcgag cggggccagg
     6481 ccgccacccg tagcgcgaca aagccgcagc ccgaaccctt gagggctcgg gctgcggccg
     6541 aggtctgacg aacatcacac tggcggaggc ggcgggattt gaacccgcga ggggttttac
     6601 ccccaaccca cttagcaggc gggcgcacta ggccactatg cgacgcctcc gtgcttccgt
     6661 acgcgacggg agccgtcaca ggttaccggg gcgagggtgc gtcgaccaac tgcgggtcac
     6721 cagaccgacg ggggcgccgt cgccgcccct cagggtgaca gtgcacaatg gaccgagccc
     6781 ggcggcatac cccgccccct gcgtccgtga ccgaacgagg tgagatgagc gacctggacg
     6841 gctacgcctc gccccgtggc gagcgctctc gccgccgcga gatcgccgaa cgagccaccc
     6901 gccgatccgc gacgctcacc ctggccacca ccatcttccc cgggctgggg ctgctgagga
     6961 cccgctacaa gcggctgggt gtcctcgtgg tgatcggggt cgttctgttc gcgatggccg
     7021 tcatcgctcg ggtagtcatg cttgggccca ctaaggccgc gctctcggtc gcggtgaacc
     7081 ccaacgctct gctcgcagcg gcggccttcg cgctcgtcgc cgggctcatc tgggtcttca
     7141 ccatcattct cgccagcctc ggcacgcgtg acccgtcgat gacccgtcgc caacgcggcg
     7201 ggctgagcgc gctcaccgcc ctgttgtgca tggcggtcat cgttccgatg ggcactgtcg
     7261 tgaactactc actcatccag cgagacgtcg tggcctcgct ctttggtgtt cagcagggga
     7321 cgccggagga ggaagagacc aacgcccagc ccggcaccgg ggtcgacccg tgggaggggg
     7381 tgagccgagt caacatgctg ttgcttggct cggatgccgg cgacgaccgg atcgggaccc
     7441 gcaccgactc gatgatcgtc gcgagcatca acccgcagac aggcgacacc ctgctgttca
     7501 gcctgccccg caaccttgag aacgtcccgt tccccaagac aaacccgctc tccaagatct
     7561 atccccaggg ctacaactgc ggcccgccgg actgtctgct caacggcgtc tggaccctcg
     7621 ccgaagccaa caagaagctg ttcggcagtg accccaaccc cggtttgacg accgttcgtg
     7681 gcgtcatcca ggagatcacc ggcctgcgcc tgcactacac gacagtcatc aacctcgatg
     7741 gtttcgagtc gctcgtggac gcgatgggtg gcgtcacagt gacgatcaac gagaaactcc
     7801 ccatcggcgg ccatctcaac tctgcgggtc agcttgtcgg cgtcgacgcg tggatccaac
     7861 cgggaaccca acacctcgac ggctatcacg cgttgtggtt cgcccgctcc cgcctagcca
     7921 gcgatgacta caaccggatg cgccgccagc gctgcctcgt cggcaagatc ctcgaccagg
     7981 tcaacccagt ctcgatgctc cagaagtacc ccgcgctggc ccaggtcgcc aaggacaaca
     8041 tcacgaccaa catcgccgcc gacgatctgc ccgcgtggat ggagttggtc cggcggatcc
     8101 agggcgcgag catcagatcg ctgacgttca cgacccaaaa catcaacccc ggcaacccga
     8161 atttcaggtt gatccgcacg atgattcagg atgcactcca ccccgcgccc gcgacggcga
     8221 gcgccagccc gacgacctcg accaagacca ccaccgggcc cacgaagtcg acgtccacca
     8281 agtcgaccaa gacctccact ggcccgacca agtcgacgac caccagttcg agcatcacca
     8341 ccgacgagct cgtcgacctc cgcgaggcgt gctgacacgg tcggatcgct cggggaccga
     8401 ccgtcagccc gtcagccgcc cgccggccgc ttggtcagtc agtgacgctg tatgccgtga
     8461 tcgacccctt cacgggcgag gtgcctctgg agatgctcgc cttgcagacc gaccccgcgg
     8521 ggacgtcgtt gaggttgacc gtctcgctgc tcgttgcacc cgaccccgcg gcataccaag
     8581 gcccgaggag cgggtaggtc tcggaggagt ccgccggccc cgcgaaccgc ccgccgaacc
     8641 agcagcggtt ctccagcgag aagtcgtaag gcacgatcgg gtagacgccg taaagggtca
     8701 cctggaccgc caacggcacc ccggccgtag gagtcccagt caccacggtg gtcccgttga
     8761 ccggaggagc ggcgtatgcc gtgccccccg gccccgcgac cagggccgcc gcggtgagaa
     8821 gggcggcacc ggcatacagg aatcgacgca tgggagtagt cctttcgcaa gccgcggcag
     8881 ccgggtgagc cgacgcggag gtcactggca gtagtcggtg gtggaggcct cgccgtagcc
     8941 gagcttccac ttcaagggcg tcgtcccggg gtcgatggtg tcggcctccc agatggcatc
     9001 ggcgatgcac tgatggccgt ccgcgtcggg atagaccccc atgcccttgg tcgtctggct
     9061 ccactcgatg agcacgccag gggtcgccgg cagcaggtag ttgggcttgg ccgtgccgtc
     9121 cgagccctcg acgaaccaac tctccgagca tccgaagttg acctccggcg agtcgtgctg
     9181 gtgcacagcg ccggactcct cgggatgctc gaccttggtc ttgatcgcca ccttcatcgt
     9241 catgcagtgg tcctcgaact tcgggttgat gtcgacgaag acatatcgcc acccgttcgg
     9301 accggcctgg aacgggccca gcgcggcctt gatcgtcgag ttgagcttgg tgaaggcgct
     9361 gtcgatcgcc gcgagcgcgg ccggtagttg cgcccaacgc accgtgcagg tcagcgccgt
     9421 gtcgatgagc gggacgcaca actgggcgat ctcgtcgacg acgtcggcgg cccggggata
     9481 ggggttggga taccccgtca ccgcgacgac caggccgggg cgctgcgacg ccatgatctt
     9541 gtcctggctg aggatcgtcg tgtagttgtt cgtcaggctg gtccacagcg tcgtattcga
     9601 ctgcaccgtc tgggcgcacg tgagggcgcc gaggaagtcg tggtccttga ccttgtcgaa
     9661 gcaactcttg atgaggttga cgatggtcga gttctgctca cccagagtga tggtgtgcag
     9721 gtccgggcgc atgttgtagg ccgacgccgc caggcccccc gagaggtagc ccgacgaggt
     9781 cgtcccgttc ttgccctggt tgtagagaca ccaccccggc gcgaccaggt ggtcggccag
     9841 cagttgctcc gggaagcggg atgggttgtc gttcaacgca tttcctgcgg ggtagtcgtc
     9901 gccgctggcg acgtagatcc ggcagccggc cgccgcctcg gcggcggggg tgcccgagag
     9961 cacccccgca cacacgaggg gaaggatggc cagcatcgct gcgcgggcgg cataccgccg
    10021 ggtatgccgt gtgctcaccg tgcgcatgtc agtctccttc gtgtctgttc gtcagtcacg
    10081 ccggtcacgg agccacaacg tcgatctgca gaccaccgtt gttgttggcg ttggtgtcct
    10141 ccccgacgac gtcacccggg tcgatcgcca cgtcgaagtc gacgaaactc ggcccacccg
    10201 tgttgacgtt ggtgacgatg gtgatcagcg acccctgacc agcggtgagc ccactcgcgg
    10261 aggcaacgca gagcacctcc ggggctgcga ggtcgccggg gaacgccggg ttcgctacgc
    10321 aggtgaagcc gttggtgccg acacccgaca cgaaggtcga ctcgttgaac gtcgtgtcga
    10381 ggttgatcgc cacgacgagc gggtcgagat ctgacgctgt cgtaccgtcg ccgatgttgg
    10441 tcaccgcgaa ctcgtaggtg acctgggccc cgtcgacccc agggttgggg tcggcggaga
    10501 tctggcccag gatcagatcg atgcacccgg tgcaggcgac gctgctgacg gtggtcttct
    10561 cggtcgccac gttgtccgtc tcgctggtct cggtgatcgc gttcgccggg tcggcggtga
    10621 cggtcacgtc gagctgaggc ggcgtggtgg aggtcgtgat gatcctgatc gacaacgtgg
    10681 tcgactcgcc tccgttgaga tctccggtgc acgtgacgat cgagtcaccg gcggtggcca
    10741 cgcagaggaa accctgcgtg ccgacggtgg cgtcgagggt caccccgacg gtggggacgt
    10801 tgacccgcac gacgacctcg tgaccgtcgg ccgcgagcgc gtcctgggtg ccgccgttgg
    10861 tgacaacgac cgtgtaggtc acggcgtcac cctgctcggc ggggtcgggc agatcggtga
    10921 tgtccccgac gaccaggttg atcgacgggc caccggcgct gaccgtgacg gagtcgctgt
    10981 tgttggcgac gctcgtctcc ggaccacagg tgctcgagat gcacggctcg gccgctgcgt
    11041 cggagtcgac cgccccggag acgctcgccg tgttggtgaa cgccgttgtg gccgtcgagt
    11101 tggcgcgcac ctcgatggtg taggtctcct gctctccggg cgcgagcgcc gtcggggtgc
    11161 aggtgaccgt gccgccggag gcggagccgt cgtggacaca ggtgccgccg ttgttgccgt
    11221 tggccgacac gaagtcgacc tcgctgggca ggacgtcttc gacggtccag cccgcggcag
    11281 ccgtggcgtc accgatgttc gaggcggtga cggtgtagga gaacgtctgc ccgatcgtcg
    11341 ccgagccgac ggatgccgac ttgtggacgg cgatgttcgg ccggaagacg tcgaccacgc
    11401 tggacttggt gtcgcagttg ttggtctcga tcgactcgac gatggtgttg gccgggtcga
    11461 cgcacgcctc gttgtccagc gtgccgccgt ccttggtgac gaagacctgc accgtgatgg
    11521 tgacgacggc cgtggcactg ccgtcggcgc ccatgtcgcc ggtgcaccgg acgaagttga
    11581 ccgggttctc gaacacctgg caggtgaagt tcgacggggt cgccgtcgca cccaggacga
    11641 tgaggccggt cggcagcgcg tcctcgacga ccacatcggt caccgcggct ccgttgttgg
    11701 tcaccgtgat cgtgtagtcc tcggtgttgt tctggtgggc cgtcgtcggg ccctccttct
    11761 cgagggtcac gtcgaagggc gagatcacga tcgaggtgtc cactgcatag ttgttggtct
    11821 cgttcgactc ggcgatggtg ttggccgggt cgacgaaggc ctggttggtg atgaccagcc
    11881 cgtgctcctg cggaggcagc accgtgatga gcacgacgcg ctcggtgggc accccggcga
    11941 tgaggtcgag gctgccgtcc agggtggcac cggtgcaggt gacgacgcca tcgctgtagc
    12001 cgcacaggaa gctcccgtta ccgccgagct ggtcg
//
LOCUS       DAOMZY010000058        11884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5032_length_11884_cov_6.307718, whole genome shotgun sequence.
ACCESSION   DAOMZY010000058 DAOMZY010000000
VERSION     DAOMZY010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 11884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11884
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5032_length_11884_cov_6.307718"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PLK64_04960"
     CDS             complement(<1..440)
                     /locus_tag="PLK64_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HPV79123.1"
                     /translation="MTRIISGLVGGRRLATPAGVSTRPTSDRVREALFSRLEHQEVLQ
                     GARVLDLYAGSGALGLEALSRGAARATLIDSDKGAARVARLNAQALGFGDVVTVRAEP
                     VERALLTGPDLADGRADLAFLDPPYDLVEDRLVDVLALLVHQRWL"
     gene            complement(440..2680)
                     /locus_tag="PLK64_04965"
     CDS             complement(440..2680)
                     /locus_tag="PLK64_04965"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPV79124.1"
                     /translation="MLAAYTEDTPLRRVVKPAQAEALAKNKGLHTVGDLLEFVPRGYL
                     PPNQLTDLGELRDGEEVMVVAEVAQVSVVPMRQRRGFRCTAIVTDGRVELDLTFFSRH
                     KYDFGLRPGIWGVFAGTVTRYGTRRQLTHPEMELLDESMSAERADYLRTHQVPVYSAT
                     GKVTSFVLRQTTSLVLDCVEEVREPLPRAVRERRGLLARGDALELIHRPRLQDDVRPG
                     MRRLRYDEAFVLQTILGQRRRAAQALHTSARMPRADGLLAAFDARLPFELTAGQREVG
                     DTIAAELAADRPMHRLLQGEVGSGKTVVALRAMLAVVDAGGQAALLAPTEVLAAQHHR
                     SISALLGDLAMGGMLGGSDLGTKVALLTGSQSTPTRRSALNDAVTGDAGIVVGTHALI
                     QKHVTFFDLALVVVDEQHRFGVEQRDALREKGKTPPHLLVMTATPIPRTVAMTVFGDM
                     ETATLTELPAGRQPITTHVVDEAKPGWVERTWARVAEEVGRGHQAYVVCPKIGEPTDG
                     TTLTADTSDEDDGWDGAGDEAGEGGIPTGPQPRPLAGVYAVHDRLRSEPALAGLRTAV
                     LHGRLDADAKDRVMTAFARGEIDVLIATTVIEVGVDVPNATVMVVMDAQQFGISQLHQ
                     LRGRIGRGSAPGLCLLMTTAPTPTAAARLDAVAATTDGFELSRLDLASRREGDVLGSR
                     QSGRGSSVRFLRMGNRDDEELIAAAREDAFAVLADDPDLAEHPILRARVAARLDDEQA
                     AYLERG"
     gene            complement(2685..4394)
                     /locus_tag="PLK64_04970"
     CDS             complement(2685..4394)
                     /locus_tag="PLK64_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HPV79125.1"
                     /translation="MPDQAPPPADRPRRVLSTLDAGGFLRWAAVARSSFASARAEIDA
                     LNVFPVPDGDTGTNLYLTLDSALDASRNAISRDIPVGSVTIPGLQAMCREMSRAMLLT
                     ARGNSGVILSQLFRGVAEHVVETGSEALNGAGLAGALASADRLAWRAVTEPKEGTILS
                     VSRAVSDRLRDAVDRDPTVPLAEVITLAVDTGTDALERTPSQLPALARAGVVDAGGAG
                     YLLLIEALARVIHGQPPQADDDPMRRHLGWSRPVLPARAHAESSGDDGGEGPAYEVMY
                     LLTDSDEERVAALRTRLSALGDSLLVVGAEGTWNVHVHVDDPGAAIEAGIEAGRPHRI
                     VVTHFGDQIKHALDPSALAVVACAAGEGIAEVFRAAGASVVLSGPGRRASAGQILEAI
                     RTSGAGQVLVLPNDGDTELAAQAAARAAAEEGQSVQVVRSRTAVQGIAALAVFDHGTD
                     LAANAQAMADAAAATRHGAVTIARKEALTAAGVCHVGDVLGVVQGEFAIIGSDLYDVA
                     GQVVQALVGDDGELLTVISGDDAPADLASAVVAAARIRRRDLEVTHIHGMQPLYPLLL
                     GVE"
     gene            4608..4796
                     /gene="rpmB"
                     /locus_tag="PLK64_04975"
     CDS             4608..4796
                     /gene="rpmB"
                     /locus_tag="PLK64_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPV79126.1"
                     /translation="MAATCDVCGKGPGFGHNISHSHRRTKRRWNPNIQRVKAIVNGVT
                     PKRLNVCTSCLKAGKVAR"
     gene            complement(4887..5822)
                     /gene="thiL"
                     /locus_tag="PLK64_04980"
     CDS             complement(4887..5822)
                     /gene="thiL"
                     /locus_tag="PLK64_04980"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HPV79127.1"
                     /translation="MTLADLTEEQLLARITPRLPGGPGVLVGPGDDTAVLRCDAATII
                     TTDAAVRGRDWRDDWSTPGDVGAKIVAQNLADVAAMGGRPLGLVVTLLADPATTVAWV
                     EGFADGLGEAARTAGAAVLGGDLSSAPRGTLAVSVTALGTLDGQAPVLRSGACAGDQV
                     AVAGTLGRSAAGLLLLERGRPELDEELVATHRRPQPPLAEGPRAGAAGAAGMLDISDG
                     LLRDGARIASASGVALDLSREALAPYAARLAPAVGADDAWECVLGGGEEHSLLACFAG
                     PVPAGWTVIGMVRTGAGVLLDGIPQAPRGWDHFRR"
     gene            5981..6214
                     /locus_tag="PLK64_04985"
     CDS             5981..6214
                     /locus_tag="PLK64_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HPV79128.1"
                     /translation="MVQAYILIQTEVGKSASVAEAIRGVAGVASAEDVTGPYDVIARV
                     EANTVDELGKLVIARIQDVPGITRTLTCTVVHI"
     gene            6325..6714
                     /locus_tag="PLK64_04990"
     CDS             6325..6714
                     /locus_tag="PLK64_04990"
                     /inference="COORDINATES: protein motif:HMM:NF023453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3515 family protein"
                     /protein_id="HPV79129.1"
                     /translation="MSAAVVVALPAGADSAPCQALTALWPSLVSSQPARAVSVQSPAA
                     RAWGNPAVIAICGYDAPAPSTLECLSVDGVDWLVQRRSDGVQFTTYGRSPAMDVLVPA
                     AYAPEPLLLPAFGAAARSLPETGRHCQ"
     gene            complement(6737..7858)
                     /locus_tag="PLK64_04995"
     CDS             complement(6737..7858)
                     /locus_tag="PLK64_04995"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HPV79130.1"
                     /translation="MSTVPPRKPRVAVVFGGRSSEHAVSCSSAASVLRAIDRQVYDVL
                     PIGITKDGRWVLAADDPAALELAPGHIPEVDGSGAGVLVPLSTTDRALQVLTPGEVPV
                     VLGDVDVVFPVLHGPWGEDGTIQGMLDLADVRYVGSGVFASAAGMDKHYMKVVFAGHG
                     LPVGPYAVITDRAWRADRGAALDAASSMGFPVFVKPSRAGSSMGITRVASPAGLKAAI
                     EAAREHDPKVLVEAAIFGRELECAVLEGRGVQPPRTSHVGEIRVVTGHDFYDFEAKYL
                     SESDVELVCPADIPDDIAVEVRRLAGAAFESLGCEGLARVDCFYTEDRRVIINEINTM
                     PGFTPHSMYPRMWAETGVPYDELVDELITLALERRVGLR"
     gene            7909..9021
                     /locus_tag="PLK64_05000"
     CDS             7909..9021
                     /locus_tag="PLK64_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HPV79131.1"
                     /translation="MTDLPPDLAAATVVVAAGRPPRSPGAPINPPIELSSTYAAHPAT
                     GPDGVALNLGYGRASNATWAAFEAAVGTLEGGDALCFASGMAAISAALTVLPADGPLV
                     APLTPYNTSGALIGEVEAAGREIRRVDVEDTVAVIAALHGARAIWLESPTNPLMQVAD
                     LPVIIAEAAARGVVVVVDNTFATPLVQQPLALGADIVVHSATKYLSGHSDLLMGVTVT
                     RAGTPDAAPDRWYAALLAHRTRHGAIAGPFEAWLALRGIRTLHVRHERACANAAELAR
                     RLGEHPSVSRVRYPGWGAMLAIEVAGSALDAERVASACQVWLGATSLGGVESLIERRR
                     RYPGESVLVPESLLRLSVGIEDVEDLWRDLDHALRA"
     gene            complement(9056..10051)
                     /locus_tag="PLK64_05005"
     CDS             complement(9056..10051)
                     /locus_tag="PLK64_05005"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HPV79132.1"
                     /translation="MSTTAAVMGTGSWGTAFAAALADAGTSVTMWGRRADSVAQINAG
                     CNEDYLPGILLPEGIRASTDPQEALAGADIVVLAVPSQTLRANLSGWTLPETAAVVSL
                     MKGVELGTTKRMSEVIMEVGGVDVERVVVVSGPNLAKEIARKQPAASVVACCDQATAE
                     RVAAACATGYFRPYFNHDVVGTELGGAVKNVVALAVGMAEGMGMGDNTKASIITRGLA
                     ETARLGVRLGADPSTFMGLAGVGDLIATCMSPLSRNRTFGENLGKGMSVAEVIEVTRQ
                     TAEGVKSCHSVLDLARNAGVEMPIAEAVTSVIDGQRTPEELVDLLMSRTRKHERI"
     gene            complement(10048..10812)
                     /locus_tag="PLK64_05010"
     CDS             complement(10048..10812)
                     /locus_tag="PLK64_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPV79133.1"
                     /translation="MRSSTSVLARMRRLPAAYQVIVVLLRPMLRVLLRRDWRGMEHLP
                     EGGCVIAPNHLSYVDPLAIALYLFDNDRPPYYLGKEAVFRIPVLGKLVGWADQIPVYR
                     GTGQAADAMRAAVAAVQEGKTVVVYPEGTLTRDPGGWPMKGKTGAARIALTTRCPVIP
                     IAMWGPQEVLGTYQKDLHLFPRKTMHILAGPPVDLSDLYGKPQDVALLAMATDRIMDA
                     ITAQLEVLRGEQAPAVRFDSRAKGVPETGNPNKERP"
     gene            10899..11549
                     /gene="cofC"
                     /locus_tag="PLK64_05015"
     CDS             10899..11549
                     /gene="cofC"
                     /locus_tag="PLK64_05015"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HPV79134.1"
                     /translation="MNDVDLRADGWVVVVPAKGGDKAKSRLTAPRGTQRHTLAAAFAL
                     DTVAAALAAVGTGRVYAVTGPGPLVALLEALGAVVVPDPGGGLNAAIRAGLAHAAAEA
                     PVRVGLAVLLADLPALTADSLSVALGAAAGVERAVVPDIDGTGSVLITATGGAVPEPA
                     FGLDSAARHAAGGQQRLDLDLPRLRTDVDDTTSLRAALSLGVGRHTTAALHHAATG"
     gene            complement(11608..>11884)
                     /locus_tag="PLK64_05020"
     CDS             complement(11608..>11884)
                     /locus_tag="PLK64_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79135.1"
                     /translation="PPAGTPSIATAPAAVKAAPAKAAVKAAPAAKKAAPAKAAPAKAA
                     PVKSVATKAAPAKAAPAAKKAAPAKAAPAKAAPAAKKAAPAKAAKKA"
BASE COUNT         1715 a         4264 c         4215 g         1690 t
ORIGIN      
        1 agccaccgct ggtgcaccag cagggcgagg acgtcgacca gtcggtcctc aaccaggtcg
       61 tagggcgggt cgaggaaggc cagatcggcg cgaccgtcag ccaggtcagg gccggtgaga
      121 agggcccgct cgaccggctc ggcccgcacc gtcaccacgt cgccgaagcc cagcgcctgg
      181 gcattgagcc gcgcgaccct ggcagcaccc ttgtccgagt cgatgagggt cgcccgggcg
      241 gcccccctgc tcaacgcctc caacccgagg gcgccggaac cggcatacag gtcgagcacc
      301 cgagccccct ggaggacctc ctggtgttcc aggcgcgaga agagggcctc gcggacccgg
      361 tcggaggtcg ggcgggtcga cacccctgcg ggagtagcca atcggcgtcc accgacgagc
      421 ccactgatga tgcgggtcat cacccacgct ccagataggc ggcttgttcg tcgtccagac
      481 gcgctgccac ccgagcgcgc aggatggggt gctccgccag gtcggggtcg tccgcgagca
      541 cagcgaacgc gtcctcgcgg gcggccgcga tgagttcttc gtcgtcccgg ttgcccatcc
      601 tcaggaaccg caccgaggag ccgcgcccgc tctgacgtga gcccagcacg tcgccctccc
      661 ggcgcgaggc gaggtccagg cgcgagagtt cgaagccgtc cgtcgtcgcc gcgacggcgt
      721 ccaaacgggc cgccgcggtg ggggtcggcg cggttgtcat cagcagacac aggccgggtg
      781 cgctccctcg cccgatccgc ccccgcaact ggtgcaactg cgagatgccg aactgctggg
      841 catccatgac gaccatgacg gtggcgttgg ggacgtccac cccgacctcg atgaccgtcg
      901 tggcgatgag cacgtcgatc tcgccgcggg cgaacgccgt catcacccga tccttggcgt
      961 cggcatcgag ccgtccgtgc aggaccgcgg tccgcagccc cgcgagggcg ggctcggagc
     1021 gcaaccggtc gtgcacggca tacaccccgg cgagggggcg cggctgcgga ccggtgggta
     1081 tgccgccctc ccccgcctcg tcgcccgccc cgtcccagcc gtcgtcctcg tcggacgtgt
     1141 cggcggtgag cgtcgtgccg tcggtcggct cgccgatctt ggggcagacg acataggcct
     1201 gatgtccgcg gccgacctcc tcagccaccc gcgcccaggt gcgctcgacc caacccggct
     1261 tggcctcgtc gacgacgtgc gtcgtgatgg gctggcgacc ggccggcaac tcggtgaggg
     1321 tcgcggtctc catgtcgccg aacaccgtca tggcgacggt ccgcgggatc ggggtggccg
     1381 tcatgacgag caggtgcgga ggggttttgc ccttctcgcg caacgcatct cgttgctcga
     1441 cgccgaaacg atgctgctcg tcaacgacga cgagggccag gtcgaagaac gtcacgtgct
     1501 tctggatcag cgcgtgggtg ccgacgacga tcccagcgtc tccggtcacc gcgtcgttga
     1561 gggccgaccg acgggtcggc gtcgattggc tcccggtgag cagggccacc ttggtgccga
     1621 ggtcggaccc tccgagcatc ccgcccatcg cgagatcgcc gagcagggcg ctgatcgagc
     1681 ggtgatgctg cgcggccaga acctcggtcg gcgcgagcag ggcggcctgc ccgccggcat
     1741 cgacgacggc cagcatggcc cgcagcgcga ccacggtctt gcccgagcca acctcgccct
     1801 gcaacagccg gtgcatcggg cggtcggcgg ccaactcggc ggcgatcgtg tcgccgacct
     1861 cgcgctggcc cgcggtcaac tcgaacggca ggcgggcgtc gaaggccgcg aggagaccgt
     1921 cagcacgcgg catacgggcg ctggtgtgca gcgcctgcgc ggcgcggcgg cgctggccga
     1981 ggatcgtctg caggacgaag gcctcgtcgt agcgcaagcg gcgcatcccc ggccggacgt
     2041 cgtcctgcag gcggggccgg tggatgagct cgagcgcgtc gcctcgggcc agcagtccgc
     2101 ggcgctcccg gaccgcgcgc gggagcggct cgcggacctc ctccacacag tccaggacca
     2161 gcgaggtcgt ctgacgcagc acgaacgagg tgaccttgcc ggtcgccgaa tagacgggca
     2221 cctgatgggt gcgcaggtag tcggcgcgtt cggccgacat gctctcgtcg agcaactcca
     2281 tctcaggatg ggtgagttga cggcgggttc cgtaacgggt gaccgtgccc gcaaagactc
     2341 cccagatgcc cggccgcagc ccgaagtcgt atttgtgcct gctgaagaac gtcaggtcga
     2401 gctcaacgcg accgtcggtg acgatcgcgg tgcaccggaa gccccgacgt tggcgcatcg
     2461 ggaccaccga cacctgggcg acctcggcga cgaccatgac ctcctcgccg tcgcgcaact
     2521 cgccgaggtc ggtcagttgg ttgggcggga ggtagccgcg cgggacgaac tccaacaggt
     2581 cgccgaccgt gtgcaggccc ttgttcttgg ccagcgcctc ggcctgagcg ggtttgacga
     2641 cccgtcgcag cggggtgtcc tcggtgtatg ccgcgagcat cggctcactc gacgccgagc
     2701 agcagcgggt agagcggctg catgccgtgg atgtgggtca cttcgaggtc gcggcggcgg
     2761 atgcgagcag cggcgaccac ggcggaggcc aggtcggcgg gagcgtcgtc gccggagatc
     2821 acggtgagca gctcgccgtc gtcacccacg agcgcctgga cgacctggcc ggcaacgtcg
     2881 tagaggtcag acccgatgat cgcgaactcc ccttggacca cgccgagcac gtcgccgaca
     2941 tgacagaccc cggccgcagt gagggcctcc ttgcgggcga tggtcacagc gccgtggcgg
     3001 gtcgcggccg cagcatcggc catcgcctgc gcgttggcgg ccagatcggt gccgtggtcg
     3061 aagacggcaa gcgcggcgat cccctggacg gcggtgcgcg accgaaccac ctggaccgac
     3121 tggccttcct ccgccgcggc gcgtgcggcg gcctgcgccg ccagctcggt gtcaccgtcg
     3181 ttgggcagca cgaggacctg gcccgcgccg ctggtgcgga tcgcctcaag gatctggccg
     3241 gccgaggccc gtcgcccggg gccactgagg acgaccgagg cgcccgcggc gcggaacact
     3301 tcggcgatcc cctcgcctgc ggcacaggcg acgactgcga gcgccgaggg gtcgagcgcg
     3361 tgcttgatct ggtcgccgaa gtgcgtgacg acgatccggt gcggacggcc ggcctcgatg
     3421 cccgcctcga tggcggctcc ggggtcatcg acatggacgt ggacgttcca cgtgccctcg
     3481 gcgccgacga ccagcaggga atcacccagc gctgacaggc gggtccgcag ggcagcaacc
     3541 cgctcctcgt cgctgtcggt caacagatac atgacctcgt atgccggtcc ctcaccgccg
     3601 tcgtcaccgc tcgactcagc gtgggcccgg gccgggagca ccggccgtga ccaccccagg
     3661 tgtcgtcgca tcgggtcgtc gtccgcctgc ggcggctggc cgtggatcac ccgggccagg
     3721 gcctcgatga gcaggagata gccggcgcca ccagcgtcga cgaccccggc gcgagccagg
     3781 gcaggcagct gggacggcgt gcgttccaag gcgtcggtac cggtgtccac ggcaagtgtg
     3841 atgacctcgg ccagaggcac ggtggggtcg cggtcgaccg catcgcggag ccggtcggac
     3901 acggcccggg acaccgacag gatggtgcct tccttgggct cggtgacggc ccgccaggcc
     3961 agccggtcgg cgctggctag tgccccggct aggcccgcgc cgttcagggc ctcgctgccc
     4021 gtctcgacga cgtgctcggc gacgccccgg aacagttggc tgaggatgac acccgagttg
     4081 ccgcgggccg tgagcaacat ggcgcgcgac atctcacggc acatcgcttg cagtccgggg
     4141 atcgtcaccg atccgacggg gatgtctcgc gaaatcgcat tgcgtgaggc gtccagggct
     4201 gagtcgaggg tgaggtagag gttggtgccc gtgtcaccgt cgggcaccgg gaagacgttg
     4261 agggcgtcga tctcggcacg cgcggacgcg aacgacgacc gggcgacggc tgcccatcga
     4321 aggaatcccc cggcgtcgag ggtgctcagc acccgccggg ggcggtccgc cggaggcggg
     4381 gcttggtcgg gcatcggacc tccttgacgc gaaccggggc cgcgagacac ggccagccta
     4441 gtcagcggtc ggtcgtcgtt ttggccccgg cgcgcccgct cggctattct tggcaagttg
     4501 ccctgttcgg tctgggcgtg cccgctgggc acgcggcata ccggcgcggg gcccccgacc
     4561 ttccagaccg cgggttcgac cccgcatcag agcatcagga gagacccgtg gcagccactt
     4621 gcgacgtttg cggcaaggga ccgggcttcg gtcacaacat ctcgcactcg caccggcgca
     4681 ccaagcgtcg ctggaacccg aacatccagc gggtcaaggc catcgtcaac ggtgtgaccc
     4741 ccaagcgcct caacgtgtgc acctcgtgcc tcaaggccgg gaaggtcgcg cgctgagcgc
     4801 ctgacccatc ggttgcagaa ggccggctcc cccatcggga gccggccttc tgctgtgccg
     4861 ggtccagccg ccgggtggaa ggggcgtcag cggcggaagt ggtcccaccc acgaggagcc
     4921 tggggtatgc cgtccagcag caccccagcc ccggtccgga ccatgccgat caccgtccac
     4981 cccgcaggca ccgggcccgc gaagcacgcc aacagcgagt gctcctcgcc gccccccagc
     5041 acgcactccc aggcgtcgtc ggcaccgaca gcgggagcca accgcgcggc atacggtgcg
     5101 agggcctcgc gcgacaggtc gagagcgacc ccgctggcgg aggcgatccg ggcgccgtcg
     5161 cgaagcagcc cgtcggagat gtcgagcatc cccgcagccc cggctgcgcc agcccgaggc
     5221 ccttcggcca gcggcggttg cggtcggcgg tgggtcgcga ccaactcctc gtcgagctcg
     5281 ggtcggcccc gctcgagcag caggagaccg gcagccgagc ggcccagcgt gcccgccacc
     5341 gcgacctggt cgcccgcgca ggccccggag cgcagcacag gtgcctgacc gtcgagcgtt
     5401 ccgagcgccg tgaccgagac ggccagcgtc ccccgcggag ccgaggacag gtcgccgccc
     5461 aggactgcgg ctccggccgt gcgcgcggcc tcacccagtc catcggcgaa cccctcgacc
     5521 caggcgaccg tcgtcgccgg gtcggccagc agggtgacga ccagcccgag cggccgtcct
     5581 cccatcgcgg ccacgtcggc gaggttttgg gcgacgatct tcgctccgac gtcacctggc
     5641 gtcgaccagt cgtcgcgcca gtcccggccg cggacggcag cgtcggtcgt gatgatcgtt
     5701 gcggcgtcgc accggagcac ggccgtatcg tcacccggcc cgacgaggac acccggaccg
     5761 ccgggcagcc ggggagtgat gcgggcgagg agttgctcct cggtcagatc ggcgagcgtc
     5821 atccccgccg ccttgccccg tgcacgccta cctggcacga ccttctcctc ctgctcaaga
     5881 cgacgaaacc ccgcccagca cggtagcgtc tgggggacgg gtcgaggacg acccgcgcgc
     5941 gggcactgcc cgccagaccc ccgacaagtg aggttgctct gtggtccagg catacatcct
     6001 gatccagacc gaggtcggca agtcggcgtc ggtcgccgag gcgatccgcg gggttgcggg
     6061 cgtcgcatcc gccgaggacg tcaccggccc ctacgacgtc atcgcccggg tcgaggccaa
     6121 cacggtcgat gagctcggca aactcgtcat cgcccgcatc caggacgtcc cgggcatcac
     6181 gcggacgctc acctgcactg tggtgcacat ctgagcggtc gctcgctgcg gtcaccaggg
     6241 cggcagaccg gcatcggccg gtatgccgcc ctgctcgctg tcccgctcgg cctcctcggc
     6301 gccgttggcg cctgttccaa cccggtgagc gcagcggtgg tcgtggccct gccggcagga
     6361 gcggacagcg ccccctgcca ggccttgacg gccctctggc cgagcctggt gtcgtcgcag
     6421 cccgcgcgag ccgtgagcgt gcagtcgccg gcggcccggg cgtggggtaa cccggcggtc
     6481 atcgccatct gtggttacga cgcccccgca ccgtcgacct tggagtgcct gtccgtcgac
     6541 ggcgtcgact ggctggtcca acgacggtcc gatggagtgc agttcaccac ctacggccga
     6601 agcccggcga tggacgtcct ggtgcccgcg gcatacgccc ctgagccgtt gttgctgccc
     6661 gccttcgggg cggcggcccg gtccctgccc gagaccggtc gccactgtca gtagcccccc
     6721 cggggcctgc gcagccttaa cgaaggccga cgcggcgctc caacgccagg gtgatgagtt
     6781 cgtcgacgag ttcgtcgtag gggacgccgg tctcggccca catccgcggg tacatcgagt
     6841 gcggggtgaa gcctggcatc gtgttgatct cgttgatgat cacccgacga tcctcggtgt
     6901 agaagcagtc cacccgggcc agcccctcgc agcccaggga ttcgaaggcc gcacccgcca
     6961 ggcgccgcac ctcgacggcg atgtcgtcgg ggatgtcggc cgggcagacc agctccacgt
     7021 cgctctcgga gaggtacttc gcctcgaagt catagaagtc gtggccggtg acgacccgga
     7081 tctcgccgac gtgcgacgtc cgcggcggct gcacgccccg gccctcgagg acggcgcatt
     7141 ccaactcgcg accgaagatc gccgcctcga cgagcacctt cgggtcgtgc tcgcgggccg
     7201 cctcaatggc cgccttgagt ccggcgggtg aagccacccg ggtgatcccc atactcgacc
     7261 cggcccgaga aggcttgacg aagaccggga agcccatcga cgaggcggcg tcgagggccg
     7321 cgccgcggtc ggctcgccaa gcgcggtcgg tgatgacggc atacgggccg acggggaggc
     7381 catgaccggc gaagacgacc ttcatgtagt gcttgtccat gcctgcggca gaggcgaata
     7441 cgccggaacc gacgtagcgc acgtcggcca gatcgagcat cccctgaatc gtgccgtcct
     7501 ccccccacgg gccgtggagc accgggaaga cgacgtcgac gtcaccgagg acgactggca
     7561 cctcgcccgg cgtgagcacc tggagggccc gatcggtcgt cgagagcggc acgagcaccc
     7621 ccgcgcccga cccgtcgacc tccgggatgt gcccgggagc cagttcgagg gcggccgggt
     7681 cgtcggcggc caacacccag cgtccatcct tggtgatgcc gatgggcagg acgtcataga
     7741 cctggcggtc gatggcccgc agcaccgacg ctgcgctgct gcacgacacg gcgtgctcgg
     7801 aggaccgtcc gccgaagacg acggcgacgc gcggtttacg gggcgggaca gtgctcatcg
     7861 gcaacgaccc tactgccccg gtatgccgtg tccgcctagg ctggccggat gaccgacctg
     7921 cccccggacc tggccgccgc caccgtcgtc gtcgccgcgg ggcgcccccc acgcagcccc
     7981 ggtgcaccga tcaatccgcc gatcgagttg tccagcacgt atgccgccca ccccgccacc
     8041 ggccccgacg gggtggccct gaacctcggc tacggccggg catcgaacgc cacctgggcg
     8101 gctttcgaag cggccgtcgg gaccctcgaa ggtggcgacg cgctgtgttt cgcctccggc
     8161 atggcggcga tctcggccgc cctcaccgtc ctcccggcgg acggcccgct ggttgctccc
     8221 ctgacgccct acaacacctc cggtgcgctc atcggggagg tggaggccgc gggccgcgag
     8281 atccgccggg tcgacgtcga ggacaccgta gcggtcatcg cggcgctgca cggtgcccgc
     8341 gcgatctggc tggagtcgcc gaccaacccc ctcatgcaag tagccgacct gccggtcatc
     8401 atcgccgaag ccgctgcgcg cggggtcgtc gtcgtcgtcg acaacacctt cgccactccc
     8461 ctggtgcagc aaccgctggc cctcggcgcc gacatcgtcg tccactcggc gacgaagtac
     8521 ctctccgggc actccgacct gctcatgggg gtcacggtca cccgcgcggg cactcccgac
     8581 gctgcccccg accgttggta tgccgcgctg ctcgcccacc gcacccggca cggggcgatc
     8641 gccggcccgt ttgaggcctg gctcgcgctg cgcggcatac gcaccctgca tgtgcgccac
     8701 gagcgggcct gcgccaacgc cgccgagttg gcgcggcgcc tcggcgagca ccccagcgtg
     8761 agccgggtgc gctaccccgg ttggggggcc atgctggcga tcgaggtggc cgggagcgcc
     8821 ctggacgccg agcgggtggc gagcgcgtgc caggtctggt tgggagccac cagcctcggt
     8881 ggggtcgagt cgctcatcga gcggcgtcgc cgctaccccg gcgagtccgt gctcgtccca
     8941 gagagcctgt tgcggttgtc cgtgggcatc gaggacgtcg aggacctctg gcgggacctc
     9001 gaccacgcac tgcgcgcctg acccaagcgg gtcaccggcc gatcagcaca gcgggtcaga
     9061 ttcgctcgtg cttgcgggtg cgcgacatga gcaggtcgac gagttcctcg ggcgttcgct
     9121 gcccgtcgat gaccgaggtg accgcctcgg cgatcggcat ctccacgccg gcgttcctgg
     9181 ccaggtcgag caccgagtga cacgacttga cgccttcggc agtctggcga gtcacctcga
     9241 tgacttcggc gaccgacatc cccttgccga ggttctcgcc gaaggtgcgg ttgcgggaga
     9301 ggggagacat gcaggtggcg atgaggtcgc ccacaccagc caaacccatg aaggtcgacg
     9361 ggtccgcccc caagcgcaca ccgagccgcg ccgtctcggc caagccgcgg gtgatgatcg
     9421 acgccttggt gttgtcgccc atccccatgc cttcggccat gccgacagcg agagcaacga
     9481 cgttcttcac cgccccgccg agctcggtcc cgacgacgtc atggttgaag taaggccgga
     9541 agtagccggt cgcgcaggca gcggccacgc gctccgcggt cgcctggtca caacaggcca
     9601 cgacgctcgc ggcaggttgt ttgcgggcaa tctccttggc caggttgggc ccggaaacga
     9661 cgacgacacg ctcgacgtcg acccccccga cctccatgat gacctcgctc atccgcttgg
     9721 tcgtgccgag ttcgacaccc ttcatcagcg agacgacggc ggccgtctcg ggcagcgtcc
     9781 agccgctgag gttggcgcgc agcgtctggg aagggaccgc gaggacgacg atgtcggccc
     9841 cggccaacgc ctcctgcggg tcggtgctgg cccggatgcc ctccggcaga aggattccgg
     9901 ggaggtagtc ctcgttgcag ccggcgttga tctgcgccac cgagtcggcc cgccggcccc
     9961 acatggtgac gctcgtgccg gcgtcggcca atgccgcggc gaacgcggtc ccccagctac
    10021 ctgttcccat gaccgcggcg gtggtgctca tggacgctcc ttgttggggt tgccggtctc
    10081 ggggacaccc ttggcccgcg agtcgaaccg cacagcgggg gcctgctcgc cccgaaggac
    10141 ttccagttgg gccgtgatgg cgtccatgat ccggtcggtg gccatggcga gcaaggcgac
    10201 atcctgcggc ttgccgtaga ggtcgctcag gtccactggc gggccggcca ggatgtgcat
    10261 cgtcttacga gggaagaggt gcaggtcctt ctggtaggtc ccgaggactt cctggggacc
    10321 ccacatagcg atggggatga ccgggcagcg ggtggtcagg gcgatccgtg cggcgccggt
    10381 cttgcccttc atcggccagc cacccgggtc gcgggtcagc gtcccctccg ggtagacgac
    10441 gacggtcttg ccctcctgca ccgccgccac cgcggcccgc atggcgtcgg ctgcctgccc
    10501 cgtcccgcga tagaccggga tctggtcggc ccaaccgacc aacttgccca ggaccgggat
    10561 ccggaagacg gcttccttgc cgaggtagta gggcggccgg tcgttgtcga agagatagag
    10621 cgcaatggcc aacgggtcga cataggacag atggttcggg gcgatgacgc atccgccctc
    10681 gggaaggtgc tccatgcccc gccagtcccg acggagcagc acacgcagca tcggtcgcag
    10741 cagcacgacg atgacctggt atgccgccgg cagccgccgc attcgcgcca gcacggacgt
    10801 ggacgacctc acacccaact ccttctcgac gcccgtatcc ttgcggcccg gggtcccagt
    10861 gtcgagcatg caccaccgga ggggagacga tgtcgctcgt gaatgacgtg gacctccgcg
    10921 ccgacggctg ggtggtcgtc gtgcccgcca agggcggcga caaggcgaag tcccgcctca
    10981 ccgcaccgcg ggggacgcag cggcataccc tcgcggcggc cttcgcgctg gacaccgtcg
    11041 ccgcggcact cgctgcggtc ggcacagggc gggtgtatgc cgtcaccgga cccggcccgc
    11101 tcgtcgccct cctggaggcg ctgggtgccg tcgtggtccc tgaccccggc ggcgggctca
    11161 atgcggccat ccgcgcgggc ctggcgcacg ctgcggccga agcaccggtg cgcgtcgggt
    11221 tggccgtgct gttggccgat cttcccgcgc tgaccgccga ctccctgagc gtggcgttgg
    11281 gcgctgccgc cggtgtcgag cgggccgtcg tgccggacat cgacggaacc ggttcggtgc
    11341 tcatcacggc gacaggcggg gctgtccccg agccggcgtt cggcctcgac tcggcggccc
    11401 gacacgccgc gggcgggcag caacgcctcg acctcgacct gccgcggctg cggaccgacg
    11461 tggacgacac gacctccctg cgggcggcgc tgtcgttggg ggtgggacgg catacgacag
    11521 ccgccctgca ccacgcggcc acgggctgac acgagcacgg gccggtctct cccgcaggag
    11581 agaccggccc ggtcgaggct cggcggttca ggccttcttc gccgccttgg cgggagcagc
    11641 cttcttggca gccggagcgg ccttggcggg agcagccttg gccggggcgg ccttcttggc
    11701 agccggcgca gccttggccg gagcggcctt cgtcgcgacc gacttcacgg gagcggcctt
    11761 ggccggagca gccttggccg gggcggcctt cttggccgcg ggcgcagcct tgacggcggc
    11821 cttggccggt gcggccttga cggcggcggg ggccgtggcg atcgacgggg tgcctgcggg
    11881 ggga
//
LOCUS       DAOMZY010000059        11871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5044_length_11871_cov_6.125169, whole genome shotgun sequence.
ACCESSION   DAOMZY010000059 DAOMZY010000000
VERSION     DAOMZY010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 11871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11871
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5044_length_11871_cov_6.125169"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="PLK64_05025"
     CDS             <1..593
                     /locus_tag="PLK64_05025"
                     /inference="COORDINATES: protein motif:HMM:NF016149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HPV79136.1"
                     /translation="LTSPPSAVATPMPYAAALAPAHSALVSAVPADQAVLASGPTELV
                     LTFNEEINPRFAQLALSRSGDVVALNPATATGTVLRATLADPGPGTYRIAYRVVSADG
                     HPISGETSFTVTGPTRTPTSTGPTPTAAASAPSTTSASSNPSAGSGAATTDSMTSATP
                     TTGSQRQDGMTWLYIGGALLALAGLTGVWESRRKRR"
     gene            691..765
                     /locus_tag="PLK64_05030"
     tRNA            691..765
                     /locus_tag="PLK64_05030"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:723..725,aa:Lys,seq:ctt)
     gene            complement(848..1144)
                     /locus_tag="PLK64_05035"
     CDS             complement(848..1144)
                     /locus_tag="PLK64_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPV79137.1"
                     /translation="MLFDRVLVSIDSEKGERTSGGGILIPATASVGKRLAWAIVVATG
                     GNVRQVQPGDRVLYDPEDRHEVELQSKGYILLREKDIHAVAASRVDDGNTGLYL"
     gene            complement(1211..1477)
                     /locus_tag="PLK64_05040"
     CDS             complement(1211..1477)
                     /locus_tag="PLK64_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HPV79138.1"
                     /translation="MSEQGQSVEQLQAEIEAARSRLSRTIDELTYRAQPQQIVARQTE
                     AAKASLMAATHDDDGGLRTERIAAVLAVVAALAIGLGLLRRRRG"
     gene            complement(1522..1989)
                     /gene="bcp"
                     /locus_tag="PLK64_05045"
     CDS             complement(1522..1989)
                     /gene="bcp"
                     /locus_tag="PLK64_05045"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HPV79139.1"
                     /translation="MTTRLQAGDIAPEFTLSDDTDQSVSLADFRGQRVIVYFYPAALT
                     PGCTTQACDFSDSLDALAAQGYAVLGISPDAPAKLAKFRAKEGLTITLLSDPDKSTLT
                     AYGAFGEKKLYGKTVVGVIRSTFVVDADGVIADARYNVKATGHVAKLRHDLGL"
     gene            2143..2844
                     /locus_tag="PLK64_05050"
     CDS             2143..2844
                     /locus_tag="PLK64_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HPV79140.1"
                     /translation="MHVPDGFLDLPTSLATAAVAASAVALSLRGARRELDDRAAPLAG
                     LVATFVFAAQMINFPVAAGTSGHLIGGALAAVLVGPWTATLCLTVVLLVQGLLFADGG
                     LSALGTNITLMGVVGVWTGYAVFRLALAVLPRRIGSVPLAAGIGGFVSVPMAALTFAG
                     LFMVGGTAPLDAGSLVAAMASVHTLIGVGEGVLTALVVGSVVAVRPDLVHGARRIRAA
                     SIQAAAPAPSSGHRA"
     gene            2841..3113
                     /locus_tag="PLK64_05055"
     CDS             2841..3113
                     /locus_tag="PLK64_05055"
                     /inference="COORDINATES: protein motif:HMM:NF024588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HPV79141.1"
                     /translation="MSGHGRRWWALGVLVTLLVAGVLSRFASTAPDGLERVAADQGIS
                     GQAVERSGLLSYDGLGGVVGVALVLAVGLGLTAVLRRRSARSPRGR"
     gene            3103..3867
                     /gene="cbiQ"
                     /locus_tag="PLK64_05060"
     CDS             3103..3867
                     /gene="cbiQ"
                     /locus_tag="PLK64_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HPV79142.1"
                     /translation="MGAEHTHGLHLPGQSTVHRLPAHLKIVSTLAFVLLVVATPREAT
                     WPYAVHLAALMLVARTARVPAAYLATRLVVEVPFVVFAVVLPFVATGPRISVLGLSVS
                     EPGLVAAWALLAKGTLGVLAALLLAATTEPRDLLTGLTRLRLPRQLVEIMGFMVRYVE
                     VVTGDLRRMSIALASRGFDARSPRQWPVLATCLGALFVRSYERGERVHLAMLSRGYAG
                     SLPVLDAAPLRVGDLVAAAALPTMAAVGMGAAVLTR"
     gene            3864..4619
                     /locus_tag="PLK64_05065"
     CDS             3864..4619
                     /locus_tag="PLK64_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV79143.1"
                     /translation="MTTPVLDVQGLYYVYPDGTRALDGADLHVHRGERVALLGPNGAG
                     KTTLVLHLNGILGGDAGSGGGTVEVSGMPVGKDTLPEIRRRVGIVFQDPDDQLFMPTV
                     RDDVAFGPANLGLRGAELEARVAEALAAVGMAAYADRPPHHLSFGQRRRVAVATVLAM
                     RPEILVLDEPSSNLDPASRRELAEIILGLDVTVLMVTHDLPYALQLCERSVILAQGRI
                     VADAPTREVLANSALMAAHRLELPFGFDPAATR"
     gene            complement(4669..4750)
                     /locus_tag="PLK64_05070"
     tRNA            complement(4669..4750)
                     /locus_tag="PLK64_05070"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4714..4716),aa:Leu,seq:tag)
     gene            complement(4784..6382)
                     /locus_tag="PLK64_05075"
     CDS             complement(4784..6382)
                     /locus_tag="PLK64_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003797900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPV79144.1"
                     /translation="MLPVPAWDAPDPSEPPGDPFGPGGWAVGNLGYAVDGLVAGATGL
                     LVAEALDVAFTLGGWVSGNSSGRSPILAVGAAFVDLTPAWLKEWAVASFGTGDKAVLF
                     AGMAIVLAVLFVAIGLVARTRHTLATALVGVLGVLALVAQLTRAGASPTDPIPLVGGL
                     AVTLWMLPRLVARTAIEGPSRREALGLAGAAAGVALATAVRGSGGVIAANASASRAAA
                     VPVPSGLPIPAAAGAEIGVGGVGPWAVPNADFYRIDTALTVPRLTAEAWSLRVWGEVE
                     REVTLTWAQLVGKPLIQRYVTLACVSNEVGGHLVGNALWTGWPVRELLREAGAKAGAD
                     MVLSRSIDGWTAGTPLSALTDDRDALLAVAMNGVALPFEHGFPVRLVVPGLYGYVSAT
                     KWVTDLKVTRFADDMGYWTPRGWSALGPVKTASRIDVPRSGARLPAGRVAVAGVAWAM
                     HRGIQAVQVQVDGGPWADARLATEPTVDSWRQWVFEWDAGPGSHVLTVRARDGAGEWQ
                     TAVQTTSDPDGATGHHSVTVAVAG"
     gene            complement(6418..7032)
                     /gene="rdgB"
                     /locus_tag="PLK64_05080"
     CDS             complement(6418..7032)
                     /gene="rdgB"
                     /locus_tag="PLK64_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HPV79145.1"
                     /translation="MTRRLVLATRNAHKVGELREILADVLADLDLQIVGLADFPEVAD
                     VVEDGVTFAENALLKARAVAAATGLPALADDSGLAVDVLGGAPGIFSARWSGRHGDDL
                     ANLELLLAQVSDVRDEHRGAGFVCAAAIALPDGRAHVEEGVFRGTLTREPRGSNGFGY
                     DPILLVEGDVRTSAELSPAEKNAMSHRGKAFRALAEHLGEMLEA"
     gene            complement(7052..7804)
                     /gene="rph"
                     /locus_tag="PLK64_05085"
     CDS             complement(7052..7804)
                     /gene="rph"
                     /locus_tag="PLK64_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HPV79146.1"
                     /translation="MSNATRHDGRAQDQTREVRITRNWLDHAEGSVLVEFGRTRVLCA
                     ASFTEGVPRWLKGQGKGWVTAEYAMLPRSTNTRSDRESVKGKIGGRTHEISRLVGRSL
                     RAVIDMKALGENTIVLDCDVLQADGGTRTAAITGAWVALADAVADAKARGLIKPDAAP
                     LTGSVAAVSVGVVGGLPVIDLDYPEDSTAETDMNVVMTGDGRFVEVQGTAEGEPFDRA
                     LLGQLLDLATKGCADVTAAQHAALAADPRSRA"
     gene            complement(7881..8642)
                     /locus_tag="PLK64_05090"
     CDS             complement(7881..8642)
                     /locus_tag="PLK64_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPV79147.1"
                     /translation="MRVTIVGCSGSFPGPQSPASCYLVQAEHEGRTWSMVLDLGSGAL
                     GPLQRYLDPTTLDAVVLSHLHADHFLDLCGLYVMQRYHPRGFRGPVPVWGPAGTPQRV
                     GLAYYGHDGTEVSEFSFTRVVDRATFRIGPFSVTARRVNHPVEAYGYRVECAGQVLVF
                     SGDTDMCDALIELAADADLLLADTAFVDGRDDERNIHMTGSRVAQVALAAGGVKRIML
                     THLPSWNDPEVCRAQAAAVWPGQVEVAAPGLVIDL"
     gene            complement(8663..9472)
                     /gene="murI"
                     /locus_tag="PLK64_05095"
     CDS             complement(8663..9472)
                     /gene="murI"
                     /locus_tag="PLK64_05095"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPV79148.1"
                     /translation="MGDAPIGIFDSGYGGLTVARAVLDQLPHESIVYFGDTARTPYGP
                     RPIGQVREFALECLDHLVDRGVKALVIACNTASAAMLHDARERYDVPVIEVIRPAVRR
                     AAAATRTNRVGVISTMGTHASRAYPDTFAAAPHIEVTSQPCPRFVEFVENGVTGGDEL
                     LAVAREYLAPLQEAEVDTLILGCTHYPLLTGVISYVMGDLVTLVSSAEETAKDVYRVL
                     ADGGALRPDGLPAPRHEFVTTGDPQTFSRLARRFLGPEAAIESRREAVAPG"
     gene            complement(9608..10222)
                     /locus_tag="PLK64_05100"
     CDS             complement(9608..10222)
                     /locus_tag="PLK64_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 domain-containing protein"
                     /protein_id="HPV79149.1"
                     /translation="MARAFRRRGARMVARLDRNEREVIASLMEQTRSLLAPDVPSTGD
                     AFTDLMASLESGATAPEGLPRGTDPGDRDPALARLLPDAHRGDPAVAAEFRRLTEQDL
                     RRRKSDALTTSIDALRADADRDRLSLEPATAQSFLIALTDTRLLMGERLGLRTEEDVD
                     AWEARVEADPDDPLAYAAALYDFLTWLQDGLVGALMGRRAFPDA"
     gene            complement(10222..10512)
                     /gene="clpS"
                     /locus_tag="PLK64_05105"
     CDS             complement(10222..10512)
                     /gene="clpS"
                     /locus_tag="PLK64_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HPV79150.1"
                     /translation="MSTAPVKEPEVISLEASELALPWITLVWNDPVNLMSYVVYVFET
                     YFGYPRAKAERLMMDVHTLGKAVVSLGTREAMETDCMAMQGFGLWATFEKNQ"
     gene            complement(10582..11238)
                     /locus_tag="PLK64_05110"
     CDS             complement(10582..11238)
                     /locus_tag="PLK64_05110"
                     /inference="COORDINATES: protein motif:HMM:NF018745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HPV79151.1"
                     /translation="MNSTGMFGPDSPRGARLGWSGVIGAVTFAVASARLGPAVAAVLG
                     WILGAGAFVLWTLVVVGRLDGPGAESHATREDSTRVVSDLILIGGSIASLGGVGVLLA
                     THRGEGGAPWQAVLGVLCVAVSWVLVHTVYLLRYAALYYAQTPRGIDFNQTEPPDYHD
                     FAYLAFTLGMTYQVSDTAISSSVFRRTVLRHTLLSYVFGSVILATTINLVVQLASPTG
                     "
     gene            11365..>11871
                     /locus_tag="PLK64_05115"
     CDS             11365..>11871
                     /locus_tag="PLK64_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79152.1"
                     /translation="MGSRLPRALIERLVDDAAVFPPGLAPLPRAVAEHLARTAYREFV
                     GPLLIPATAAAEVRELAGGAHLRVGLIARPGMPVEPVRAAAAALAGSTVEIAGVEVGA
                     GAEWESLLDLGVALTVEIPRDGFTPAIDAVAAVALRTGREPLVQAKFRTGATEVWAWP
                     DEVELARFF"
BASE COUNT         1755 a         4260 c         4096 g         1760 t
ORIGIN      
        1 cactcacctc gcctcccagc gccgtggcca ccccgatgcc gtatgccgcg gcgctcgcgc
       61 ccgcgcacag cgccctcgtg agcgccgtcc ccgccgacca agcggtgctg gcgtcggggc
      121 cgaccgagtt ggtgctgacc ttcaacgagg agatcaaccc ccgcttcgcc cagctggccc
      181 tcagtcgctc gggcgatgtc gtcgcgctca acccggcaac cgctaccggc acggtgctgc
      241 gcgccacctt ggccgacccg ggccccggga cctaccggat cgcctaccgc gtcgtcagcg
      301 ccgacggaca ccccatctcc ggcgagacga gtttcaccgt cacgggcccc acccggacgc
      361 cgaccagcac cggacccacc ccgaccgccg cagccagcgc cccgagcacg acgagcgctt
      421 ccagcaaccc cagcgccggc tcaggcgcag cgacgaccga cagcatgacc agcgcgacac
      481 caacgaccgg atcgcaacgc caggacggta tgacctggct ctacatcggc ggcgccctgc
      541 tggctctggc cggcctcacg ggcgtctggg aaagccgccg caagcgtcgt tgacccaggc
      601 gcagcgtcgt tcgccacgct cgcaggaggg tacggcagga accgatttcc accacgcccg
      661 acccagcgtg tagagtttcg gctcggccca gcgccgctag ctcaatggca gagcaagtga
      721 ctcttaatca ctgggttcgg ggttcgaatc cctggcggcg caccacacga gggcggttcc
      781 tacgggagcc gccctcgcgg catacctggg ggtcacctgg ctgccgaagc accctcgcga
      841 gcgcccttca gaggtagagg cccgtgttgc cgtcatcgac gcgcgaggcc gcaaccgcgt
      901 gaatgtcctt ctcgcgcaac aggatgtaac ccttggactg caactcgacc tcgtgccggt
      961 cctcggggtc gtagagcacc cggtcacccg gctgcacctg ccggacattg ccgccggtgg
     1021 ccacgacgat cgcccaggcc agtcgtttgc cgaccgacgc cgtagcgggg atgaggatgc
     1081 cgcctccgga ggtgcgttcg cccttctcgg agtcgatgga gaccagaacc cgatcgaaga
     1141 gcattcggat gggcaaccgg tcagaagtgc tggccgccgg tgcggcacta ggtggcatct
     1201 cggcggtgga ctagccccgg cgccggcgca gcaggcccag cccgatggcc aaggccgcga
     1261 cgaccgcgag gacggccgcg atgcgctcgg tgcgcaggcc gccgtcgtcg tcgtgggtcg
     1321 ccgccatgag ggatgccttc gcggcctcgg tctggcgggc gacgatctgc tgcggctgtg
     1381 ctcgataggt caactcgtcg atggttcggg acagccggga gcgagccgcc tcgatctccg
     1441 cttggagctg ttcgaccgat tgtccctgct cgctcatcga gcgctccctt cgtcctgggg
     1501 caaagcctaa tgggcgggcg ctcagaggcc gaggtcgtgc cggagcttgg cgacgtgacc
     1561 ggtggccttg acgttgtatc gagcgtcggc gatgaccccg tcggcgtcca cgacgaacgt
     1621 cgaacggatg actccgacga cggtcttgcc gtagagcttc ttctcgccga aggcgccgta
     1681 ggccgtgagc gtcgacttgt cggggtcgga caacagcgtg atcgtcagcc cctccttggc
     1741 gcggaacttc gcgagcttgg ccggggcatc cggcgagatg ccgagcacgg catacccctg
     1801 cgcggcgagc gcgtcgaggg agtcggagaa gtcgcacgcc tgggtggtgc acccgggagt
     1861 gagggccgcc gggtagaagt agacgatgac ccgctgcccg cggaagtcgg ccagcgagac
     1921 cgactggtcg gtgtcgtcgc tgagggtgaa ctcgggggcg atgtcgcccg cctgcaagcg
     1981 tgtcgtcacg gtgactccca cggtcgacta ttgcgaatga tttgcaataa gatctgagga
     2041 tggctgggat tcgacccgct cgggtcgccc tgtcggacac cgtaccccgc cccgccgaca
     2101 gcagccgccc cgcccgatcc gccaagccgc aaggaggccg ccgtgcacgt ccctgacggc
     2161 ttcctcgatc tgccgacctc cctggccacc gcggccgtcg ccgcgagcgc tgtcgcactt
     2221 tccctgcgcg gcgcacgccg cgagctcgac gatcgagccg ccccgctggc cggtctcgtc
     2281 gccaccttcg tcttcgccgc ccagatgatc aacttccccg tggccgccgg cacgtcgggt
     2341 cacctcatcg gcggcgcgct cgccgcggtc ctcgtcggac cgtggacggc gacgttgtgc
     2401 ctgaccgtcg tcctgctcgt gcagggtctg ctcttcgccg acggcgggct cagtgcgctc
     2461 gggacgaaca tcactctcat gggcgtggtt ggggtgtgga ccgggtatgc cgtgttccgc
     2521 ctcgcgctgg ccgtcctccc ccggcggatc ggctcggtgc cattggccgc cgggatcggt
     2581 ggcttcgtct cggtgccgat ggccgccctc accttcgccg gactgttcat ggtcggcggg
     2641 acggcgcccc tggacgccgg ctcgttggtg gcagcgatgg ccagtgtcca caccctcatc
     2701 ggtgtcggcg aaggagtcct caccgcgctg gtcgtcggca gcgtggtcgc cgttcgcccc
     2761 gacctcgttc acggcgcccg ccgcatccga gccgcgtcga tccaggctgc cgccccggct
     2821 ccgtcgtccg gacaccgggc atgagcggtc acggtcgccg gtggtgggcg ctcggcgtgc
     2881 tggtgacgct gctggtggcc ggggtcttga gccgcttcgc gtcgaccgct ccggacgggc
     2941 tcgagcgggt ggccgcagat cagggcatct ccggccaggc cgtcgaacgg tccggcctct
     3001 tgtcctatga cggcctcggt ggggtggtgg gagtcgccct ggtcctcgcc gtcgggctcg
     3061 ggctcaccgc ggtcctgcgc cgtcgttcgg caaggtcacc gcgtgggcgc tgagcacacg
     3121 cacggcctgc acctgcccgg tcagtcgacc gttcaccggt tgccggcgca cctcaagatc
     3181 gtctcgaccc tcgccttcgt cctcctcgtc gtcgccacac cccgcgaggc cacctggccc
     3241 tatgccgtcc acctcgccgc cctcatgctg gtcgctcgga cggcgcgggt gcccgcggca
     3301 tacctcgcca cgcggctcgt cgtggaggtg cccttcgtcg tgttcgccgt ggtcctgccg
     3361 ttcgtcgcga cggggccacg catcagcgtg ctgggtctct cggtctcgga accgggcctg
     3421 gtcgcggcgt gggcgctgtt ggcgaaggga accctcggcg tgctcgccgc cctcctgctc
     3481 gcagcgacca ccgagccacg cgacctgctc accggcctca cccggctgcg cctgccccgt
     3541 caactcgtcg agatcatggg cttcatggtt cgttacgtcg aggtcgtcac cggcgacctg
     3601 cggcggatgt cgatcgccct ggcctcccgc gggttcgatg cccgctcgcc gcggcagtgg
     3661 ccggtgctcg ccacctgcct cggagcgctc ttcgtgcggt cctacgagcg gggcgagcgg
     3721 gtccacctgg cgatgctctc gcgcgggtat gccggctcgc ttccggtcct cgacgcggcc
     3781 ccgctgcgcg tgggagacct ggtggcggcg gccgctctgc cgacgatggc cgcagtcggg
     3841 atgggcgcgg cggtgctgac ccgatgacga cccccgtgct cgacgtccag ggcctctact
     3901 acgtctaccc ggacggcacc cgcgccctcg acggggcgga cctgcacgtc catcgtggcg
     3961 agcgggtggc gctgcttggc cccaacggcg cgggcaaaac caccctcgtc ctgcacctca
     4021 acggcattct cgggggcgac gcgggctccg ggggcggcac cgtcgaggtg tccggtatgc
     4081 cggtcggcaa ggacacgctc ccggagatca ggcgccgcgt cgggatcgtc ttccaagacc
     4141 ccgacgacca gttgttcatg ccgaccgtcc gcgacgacgt ggcgttcggt ccggccaacc
     4201 tcgggctgcg cggagccgag ttggaggctc gtgtcgctga agcgctcgcg gcggtgggga
     4261 tggcggcata cgccgatcgg ccgccacacc acctgtcctt cgggcaacgc cgccgcgtcg
     4321 cggtggcgac ggtcctggcg atgcgcccgg agatcctcgt cctcgacgag ccgtcctcca
     4381 acctcgaccc cgccagccgc cgcgagttgg ccgagatcat cctcgggctc gacgtgaccg
     4441 tcctcatggt gacgcacgac ctgccttacg ccctgcagct gtgcgagcgg tcggtcatcc
     4501 tcgcgcaggg ccggatcgtc gccgacgccc cgacccgaga ggtgctggcc aacagcgcac
     4561 tcatggcggc gcaccggctc gagttgccct tcggcttcga ccccgccgcg acccgctgac
     4621 tcgcgtccga cgaggcgacg ccgaggcgcc gaggcgcccg agagagagtg cgcgcggcgg
     4681 gactcgaacc cgcacacccg aaggcaccag aacctaaatc tggcgcgtct gccaattccg
     4741 ccacgcgcgc gcggcgccaa ctctagccaa gcgcgcgcag gtctcagccc gccaccgcca
     4801 ccgtcaccga gtggtggccg gtcgctccgt ccgggtcgga tgtggtctgc acggcggtct
     4861 gccactcccc cgccccgtcg cgggctcgga ccgtgaggac atgcgatccg ggcccggcgt
     4921 cccactcgaa cacccactgc cgccatgagt cgacggtcgg ttcggttgcc agtcgcgcgt
     4981 cggcccacgg cccgccgtcg acctgcacct gcaccgcctg gatgccgcga tgcatggccc
     5041 acgcgacgcc agccaccgcg acccgaccgg caggcaggcg agcccccgag cgcgggacgt
     5101 cgatgcgcga ggcggtcttg actggcccga gggccgacca gcctcgcggc gtccagtagc
     5161 ccatgtcgtc ggcgaaccgg gtgactttga ggtcggtcac ccacttggtg gccgacacgt
     5221 agccatagag gcccgggacg accagacgca cggggaagcc gtgctcgaac ggcagcgcca
     5281 ctccattcat cgcgacggcg agcagagcgt cgcggtcatc ggtgagcgcc gacagcggtg
     5341 tcccggcggt ccagccgtcg atactgcgcg agaggaccat gtcggcgccc gccttggcgc
     5401 cggcctcgcg cagtagttcg cgcaccggcc acccggtcca gagggcattg ccgacgagat
     5461 ggccaccgac ctcgttcgag acgcacgcga gggtgacgta gcgctggatg agcggcttgc
     5521 ccaccagttg cgcccaggtc agcgtcacct cacgctcaac ctcaccccag acccgcaacg
     5581 accaggcttc ggccgtcaac cgcgggacgg tcaacgcggt gtcgatccgg tagaagtcgg
     5641 cgttcggcac cgcccacggg ccgaccccac cgaccccgat ctcggccccc gcagccgccg
     5701 gaatcggcag accggagggc acggggacgg cggccgctcg tgacgcgctc gcgttggcgg
     5761 cgatgacacc gccggacccg cgcaccgcgg tcgccagcgc gacaccggct gcggcgccgg
     5821 ccaggccgag ggcctctctg cggctgggcc cctcaatcgc ggtgcgggca accaaccgcg
     5881 gcaacatcca gagggtcacg gccaagccgc cgaccagcgg gatcgggtcg gtgggcgagg
     5941 ctccggcgcg cgtgagttgg gcgaccaagg ccagcacgcc cagcaccccg acgagggcgg
     6001 tcgcgagggt atgccgcgtg cgcgcgacca gcccgatggc cacgaagagg acggccagga
     6061 cgatcgccat gccggcgaac aggacggcct tgtcgccagt gccgaaactg gcgacggccc
     6121 actccttgag ccacgcgggc gtgaggtcga cgaacgcggc gcccacggcc aggatcgggg
     6181 accgtcccga ggagtttccg ctcacccacc ccccgagggt gaacgcgaca tcgagcgcct
     6241 cagcgacgag cagcccggtc gctccggcga ccaggccgtc gacggcatac cccaggttgc
     6301 cgaccgccca cccacccgga ccgaacgggt cgccgggcgg ctcggacggg tcgggggcgt
     6361 cccaggcggg cactggcagc acgccctgag gccctctttg gccgggctgt tcggaggtca
     6421 cgcctccagc atctctccca gatgctccgc cagagcccga aaagccttac cgcggtgtga
     6481 catcgcgttc ttctcagcgg gcgaaagctc ggccgacgtc cgcacgtcac cctcgacgag
     6541 caggatcggg tcatagccga agccattgct gccgcggggt tctcgggtca acgtcccccg
     6601 gaagacgccc tcctcgacgt gcgcccggcc gtccggcagc gcgatcgccg ccgcacagac
     6661 gaagcccgcg ccgcggtgct cgtcccggac gtcggacacc tgggccagca gcaactccag
     6721 gttggccaga tcgtcgccgt gccgtcccga ccaccgcgcc gagaaaatgc ccggagcacc
     6781 cccgaggacg tcgacggcca gaccggagtc gtcggccagc gcgggcaggc cggtggccgc
     6841 agcgacagcg cgggccttga gcagggcgtt ctcggcgaag gtcacgccgt cctcgacgac
     6901 gtcggcaact tccgggaaat cggcgagccc gacgatctgc aggtcgaggt cggcgagcac
     6961 gtcggcgagg atctcgcgaa gttcaccaac cttgtgggcg ttgcgggtgg ccaggacgag
     7021 gcggcgggtc acggcatacc cctggtcggg ttcaggcgcg cgagcgcggg tcggcggcga
     7081 gcgccgcgtg ttgcgcggcc gtgacgtcgg cacagccctt ggtcgcgagg tcgagcagtt
     7141 gccccagcaa cgcccggtcg aagggctccc cctcggcggt cccctggacc tcgacgaagc
     7201 gcccgtcgcc ggtcatgacg acgttcatgt cggtctcggc cgtggagtcc tcggggtagt
     7261 ccaggtcgat caccgggagg ccgccgacga caccgacgga caccgcggcc accgatccgg
     7321 tgaggggcgc ggcgtcaggc ttgatgagcc cccgagcctt ggcatcggcg accgcgtcgg
     7381 ccaaggccac ccaggccccg gtgatagcgg ccgtccgggt gccgccatcg gcctgcagca
     7441 catcgcagtc caacacgatc gtgttctcgc ccaaggcctt catgtcgatg acggcgcgca
     7501 gcgagcggcc gacgagccgg ctgatctcgt gggtgcggcc accgatcttg cccttgaccg
     7561 actcccggtc cgaacgggtg ttggtcgagc gcggcagcat ggcgtactcc gccgtcaccc
     7621 agcccttgcc ctgccccttg agccagcgcg gaaccccctc ggtgaacgac gccgcacaca
     7681 gcacgcgggt ccgtccgaac tcgacgagca ccgacccctc ggcgtggtcg agccagttgc
     7741 gcgtgatgcg cacctcgcgg gtctggtcct gggcgcggcc gtcatggcgg gttgcgttgc
     7801 tcacggcgga cagactacgc aggtatgccg tgctcccccg ccgcgcgtcc cgaccggtga
     7861 tcgagtgggc gcgtcgaccg tcagaggtcg atgaccaggc cgggggcagc gacctcgacc
     7921 tgtccgggcc acaccgcggc ggcctgggcg cggcatacct ccgggtcgtt ccaggacggc
     7981 aggtgcgtga gcatgatccg cttgacgcct ccggcggcga gggcgacctg cgcgactcgt
     8041 gagccggtca tgtggatgtt gcgctcatca tcgcggccgt caacgaaggc ggtatcggca
     8101 aggagcaggt cggcatcggc ggcgagttcg atcagcgcgt cgcacatgtc ggtgtcgcca
     8161 ctgaacacca gcacctggcc tgcgcactcg acccgatagc cgtaggcctc gacggggtgg
     8221 ttgacccggc gcgcggtgac cgagaacggg ccgatgcgga aggtcgctcg gtcgacgacg
     8281 cgggtgaagg agaactccga cacctcggtg ccgtcatgcc cgtaataggc gagcccgacc
     8341 cgttgtggcg taccggccgg accccacacc ggcacgggtc cgcggaagcc tcgcgggtgg
     8401 tagcgctgca tgacatagag cccacacagg tccaggaagt ggtcggcgtg caggtggctg
     8461 aggacgacgg catccagggt cgtggggtcg aggtaacgct gcaacggccc gagggcccca
     8521 ctgcccaggt cgagcaccat gctccatgtc cggccttcgt gctcggcctg gacgaggtag
     8581 cacgaggccg gcgactgcgg cccggggaag gaccccgagc acccgacgat cgtgacgcgc
     8641 atccgtggtg aggtctctgc gctcatcccg gcgcgaccgc ctcccggcgc gactcgatcg
     8701 ccgcctccgg tccaaggaac cgccgggcca gtcggctgaa ggtctgcgga tcacccgtcg
     8761 tgacgaactc gtgtcgcggc gctggcaacc cgtcggggcg cagcgcgccg ccgtcggcca
     8821 gcactcggta aacatccttg gccgtctcct cggccgacga aacgagcgtg accagatcgc
     8881 ccatgacgta ggagatcacg ccggtgagca gggggtagtg ggtgcaccca aggatgaggg
     8941 tgtcgacctc ggcttcctgc aacggcgcta ggtattccct tgcgacggct aacaattcat
     9001 cgccaccggt cacgccattc tcaacgaact cgacgaaccg cgggcacggc tgcgacgtca
     9061 cctcgatgtg tggcgcggcc gcgaaagtgt cggggtaggc acggctggca tgggttccca
     9121 tggtcgagat gacacccacc cggttggtgc gagtcgctgc ggccgcccgg cggaccgccg
     9181 gccggatcac ctcgatgacc ggcacgtcgt agcgctcgcg agcatcgtgc agcatcgcgg
     9241 cgctggcggt gttgcacgcg atgaccaagg ccttgacccc acggtcaaca aggtggtcga
     9301 ggcactccaa cgcgaactcg cgcacctgcc cgatcggccg cgggccgtag ggcgtgcggg
     9361 ccgtgtcacc gaagtagacg atggactcgt gcggcagttg gtccaggacc gctcgggcaa
     9421 cggtcaatcc gccgtaaccc gagtcgaaga tcccgattgg cgcatcgccc acggcactca
     9481 ggataaggag gtatgccgct cgtcccacaa tcgtgggtcg gtcacctcgg tcacatcgtc
     9541 gtacacctcg gtgccgtcca cgttcaggcc tccgtcacgt tctcttcatg cgtcggtcac
     9601 ttgtcggtca cgcatcgggg aaggcccggc ggcccatgag ggctccgacg agcccgtcct
     9661 gaagccaggt gaggaagtcg tagagggctg ccgcataagc caacggatca tccgggtcgg
     9721 cctcgacccg tgcctcccac gcgtcgacgt cctcctcggt gcgcagcccc agtcgctcgc
     9781 ccatgagcag tctggtgtcg gtgagagcga tgaggaacga ctgtgcggtc gcgggctcca
     9841 ggctcagccg gtcgcgatcc gcgtcggcac gcaaggcatc gatcgatgtt gtcagcgcgt
     9901 cgctcttgcg ccgccgaagg tcctgctcgg tgagcctgcg aaactccgcc gccaccgcag
     9961 ggtccccccg atgtgcatcg ggcaataacc tggccagcgc ggggtctcga tcgcccgggt
    10021 cggtcccccg aggcaggccc tccggggcgg tcgcccccga ctccaaggac gccatcaggt
    10081 cggtgaaggc gtctccggtc gagggaacgt cgggagccag cagcgaccgg gtctgctcca
    10141 tgaggctcgc aatgacctcg cgctcgttgc gatccagccg ggcgaccatg cgggcaccac
    10201 ggcgccgaaa cgcgcgggcc attactggtt cttttcgaag gtcgcccaga ggccgaagcc
    10261 ctgcatggcc atgcagtcgg tctccatcgc ctcgcgcgtg cccagggaga cgaccgcctt
    10321 ccccaaggtg tgcacgtcca tcatcagcct ctcggccttg gcccgcgggt agccgaaata
    10381 ggtctcgaag acgtagacca cgtatgacat gaggttgacc gggtcgttcc agaccagcgt
    10441 gatccaggga agcgccaact ccgaggcctc aagcgagatc acctcgggtt ctttgactgg
    10501 tgccgttgac acgggtgaca gtgtaggcgc acggggggac gccgggaatg ccccattttg
    10561 gcgccccaca gacgcctcga atcaccctgt cggactggcc aattggacga cgaggttgat
    10621 ggtggtggcg aggatcaccg agccgaagac gtaggacagc agggtgtggc gcagcacggt
    10681 ccgccggaag acggagctgc tgatcgcggt atcggacacc tgatacgtca tgccgagggt
    10741 gaaggcgagg taggcgaagt cgtgatagtc cggcggttcg gtctggttga agtcgatccc
    10801 tcggggcgtc tgggcgtagt acagcgcggc atacctcagc aggtagaccg tgtggacgag
    10861 cacccaggac accgcgacgc acagcacccc gagcaccgcc tgccagggcg ccccgccctc
    10921 cccgcggtgc gtggcgagca ggacgccgac accgccgaga ctggcgatgc ttcccccgat
    10981 gaggatgagg tcactgacga cacgggtcga gtcctcacgg gtggcgtggg actcggcgcc
    11041 gggcccatcc agacgcccaa cgaccacgag ggtccagagg acgaacgctc cagcaccgag
    11101 gatccagccg aggacggcgg cgaccgcggg acccagccgc gccgacgcga cggcgaaggt
    11161 cacggcgccg atcacgcccg accagcccag gcgtgcacct cgggggctgt cggggccgaa
    11221 catgccggtg gagttcatca cgacaggtga cgctatctcc ccgagccggc ggactcgggg
    11281 gtgcgcaacc tgcgcaggtt tcccgtggtt ggcagcgacc cgcgggccgc tgtcggccag
    11341 gcctggtggg ctagcctgcc aaacatggga tcccggctgc cgcgagcact gatcgagcgg
    11401 ctggtcgacg acgccgcggt cttcccgccc ggcctggctc ccctcccgcg cgccgtcgct
    11461 gagcatctgg cgcgcacggc ataccgcgag ttcgtggggc cgttgctgat ccccgcgaca
    11521 gcggctgcgg aggtccgcga gctcgccggc ggagcgcacc ttcgcgtcgg cctgatcgcc
    11581 cgccccggta tgccggtcga gccggtccgc gccgcagccg ccgcccttgc cggcagcacc
    11641 gtcgagatcg ccggcgtcga ggtcggagcc ggggctgagt gggagagcct gctggatctt
    11701 ggggtagcgc tcacggtcga gatcccgcgc gacgggttca cccccgcgat cgacgccgtc
    11761 gccgctgttg ccctccggac cggtcgcgaa ccgttggtcc aggcgaagtt ccgcacgggt
    11821 gccaccgagg tctgggcctg gcccgacgag gtcgagttgg cccgcttctt t
//
LOCUS       DAOMZY010000060        11825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5069_length_11825_cov_6.404418, whole genome shotgun sequence.
ACCESSION   DAOMZY010000060 DAOMZY010000000
VERSION     DAOMZY010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 11825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11825
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5069_length_11825_cov_6.404418"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..3984
                     /locus_tag="PLK64_05120"
     CDS             175..3984
                     /locus_tag="PLK64_05120"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HPV79153.1"
                     /translation="MPDQAKSDNPMGAFGPNEWLVDELYDQWLNDKDSVDRAWWEFFE
                     DYRPGHPSGPLGNGHRAAPAAPAPAPSLPVSAPTPAPAPAPSTPVVAGSAGTPGAPVA
                     PGAAPLAAPAPTAPMPRDIPAPKSTPPPSEAEIRPLRGPAARVVVNMQSSLEVPTATS
                     VRTVPAKLLIDNRVVINNHLARSRGGKVSFTHIIGYALVKALKALPAMNHAFGEQDGK
                     PVVIAPAHVNLGLAIDLAKPDGTRQLLVPSIKSAELMDFVHFWSAYEDMVKKARGGKL
                     TVDDFAGTTISLTNPGTIGTVHSVPRLMQGQGTIVGVGAMEYPAEWQGASNETLNRNA
                     VSKILTLTSTYDHRIIQGAQSGDFLRIVHHLLLGGDRFYDEIFESLRIPYEPIRWVQD
                     VTATHDDDINKVARVQEIIHAFRVRGHLMADTDPLEYRQRRHPDLDITEHGLTLWDLD
                     RHFATGGFGGKPFLKLRQILGILRDSYCRTVGIEYMHIQDPTQRKWIQDQCEVVHEKP
                     TRDEQMRILRRLNAAEAFETFLQTKFVGQKRFSLEGGESAIALLDRILSAAAEGGMEE
                     VCIGMPHRGRLNVLANIAGKSPAQIFREFEGKADPKSVQGSGDVKYHLGTEGTFTAEN
                     GASTKVYLAANPSHLEAVNPVLEGIVRAKQDRLDLGGDAYTVLPILMHGDAAFAGQGV
                     VAETLQQSELRGYRTGGTIHVIVNNQVGFTTAPSASRSSVYSTDVARMIQAPIFHVNG
                     DDPEACVRVAELAFEFRQKFKKDVVVDMVCYRRRGHNEGDDPSMTQPLMYNLIEAKRS
                     VRKLYTESLIGRGDISVEDASAALRDYQQQLERVFLETREAQAAPAASQTAYVDSTSD
                     VAGHGGLEKPTAQALDDVSRPRDWRQTAISVDTLNTLGDAWVRPPDGFTVHPKLKALM
                     DRRAAMVREGGIDWGMGELLALGSLLMEGTPVRLAGQDSQRGTFVQRHAVLIDKITGR
                     EWTPLHHLQEGQAKFWVYNSLLSEYAAMGFEYGYSVERPDALVLWEAQFGDFANGAQT
                     IVDEFISSSEQKWAQRSSVVLLLPHGYEGQGPDHSSARIERFLQLFAEDNMTIAYPST
                     PASHFHLLRRQAYARPRRPLIVFAPKSMLRLKAAASMPQDFTTGGFRPVLPDTRHLDG
                     GAVTRVLMASGKVVWELEAERDKLGDTSTALLRIEQLAPAPAEELAAALRGYPNAELV
                     WVQDEPKNQGAWPFMALNLPELLAEQGENRPLRVIARKASASPASGSHKKHELEQAAL
                     LGAAFAR"
     gene            4196..5314
                     /locus_tag="PLK64_05125"
     CDS             4196..5314
                     /locus_tag="PLK64_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012245540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPV79154.1"
                     /translation="MTDDLDELSSLARTFFAREVTPNAERFAEQHQVDRETWLKAGGA
                     GLLGLSVPEEFGGGGGMFAHEAVVLQEQGWAGDDALGLHIHSGIVPQYIVEFGTLEQK
                     RRWLPKLASGEWVGAIAMTEPGTGSDLQAVRTTARRDGDDYVVNGSKTFITNATHADL
                     VIIVCRTGAPGAKGISLLVAEVNDLPGFTRGRVLAKIGQHGQDTRELAFSDMRVPAAN
                     LLGEEGHGFGQLMEQLPQERLVVAVGAVAAAEYAVRLATAYSKEREAFGRKILDFQHT
                     RFVLSEVSTDALAARTFLDHCITEHLAGRLDAVLASRIKYWATDVQCSVIDRCLQVFG
                     GYGYMMEYPIARLYAAARVQKIYAGTNEIMKELISRSL"
     gene            5411..5587
                     /locus_tag="PLK64_05130"
     CDS             5411..5587
                     /locus_tag="PLK64_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002518904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6104 family protein"
                     /protein_id="HPV79155.1"
                     /translation="MYFTDRGIEELDARRGDEEVTFSWLAEHLRTFVDLHPEFETPVE
                     RLATYLARLDDDEE"
     gene            5610..6296
                     /locus_tag="PLK64_05135"
     CDS             5610..6296
                     /locus_tag="PLK64_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPV79156.1"
                     /translation="MTGHDGPAYPSATDGEAAPEFTPSASFTVDDDGRRDIGLCFVGD
                     SFTVGFGDPKALGWVGRVVSRTHHPDAEVTAYNLGVRGQDSGDVMARWRGECMPRWVG
                     RGERRLVVAVGANDLGQGLTTARSRLNLANILDDASAKGIATFVVGLTPSLDPDLNAR
                     IGVLAQAQADVCSRRGVTYVDCYRPLVAHEQWAADLAAGDGVHPGQAGYGLIAWLVLH
                     AGWNAWMQLG"
     gene            6402..7406
                     /locus_tag="PLK64_05140"
     CDS             6402..7406
                     /locus_tag="PLK64_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HPV79157.1"
                     /translation="MAEQWLVDGPRVIDVGSDRERVEKVVVGIIGGRVNVVTHDDGYG
                     ARVEVSNVEGLPLRVSWDGRTLKVMHTKDGGSTVLDTLRRLVEQVSGQHADVSISVPA
                     SARATVSTVTAPIVVSGLRRGLAANTVSGEMTISDVQGRSTINTVSGATECADLVGDA
                     KINSVSGSVTVQSSDIPRARINTVSGEVALDLVNTHLDVQSNSVTGDVTIRAPFSGYA
                     VTATSATGQVIVDGQSLHGASSGSMPPGGWGDCAPGGSSGRGTLRSGDESLTLRANSV
                     SGDVILLRAAAPAAGPSGSGDAGPQDRSPEVSPGGPQDAPARDEVRESSIDDEGQDTP
                     "
     gene            7403..8164
                     /locus_tag="PLK64_05145"
     CDS             7403..8164
                     /locus_tag="PLK64_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPV79158.1"
                     /translation="MSPVFAHGQLRLYLLVLLAEAPRHGYEVIQDLETRFGGLYTPSA
                     GTVYPRLAKLEEEGLVERTDEGRKATYRITEAGLAEVRARHREVEDLQADLDQSVRRL
                     AEQVRATVQGRSADLRAELKAAAREARTTARPAGSTSGTGWPQGSSPGWGQSPTDRRA
                     GAGAYGGTGDRSGSRSDSGSGAGSGRASGGGSGAGTFAGADWTDVERAASEVREQARR
                     AWRQHGLTAEQTAEIVEILADTSRRIAQVLRMPRD"
     gene            complement(8178..8564)
                     /locus_tag="PLK64_05150"
     CDS             complement(8178..8564)
                     /locus_tag="PLK64_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79159.1"
                     /translation="MSTGGFRPRVSSARRVAAIICAIEALAMAGFGIYGLVELARGGG
                     TGTARVITESVLILAFAAGMALLARLWLGRSGWPGTPTVVWHALLIPVVVSMAQAGQW
                     LVTLSLSVAIVLSIGAATVARGSGSD"
     gene            8928..9176
                     /locus_tag="PLK64_05155"
     CDS             8928..9176
                     /locus_tag="PLK64_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HPV79160.1"
                     /translation="MDWRDRAACLDEDPELFFPIGNTGPAILQIEEAKAVCRRCEVLD
                     TCLRWALESGQDAGVWGGLSEDERRALKRRNARARRAG"
     gene            complement(9279..10745)
                     /locus_tag="PLK64_05160"
     CDS             complement(9279..10745)
                     /locus_tag="PLK64_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HPV79161.1"
                     /translation="MATLSELIQEHAALPQVDQEWLRLLVSDWQLLSDLSFADLVLWV
                     RSSPDRWLAVAHIRPTTGQTVYFRDQVGRVAGTSRVELLEAAYLQRRIVRERDSEWDP
                     DDPVREESIPVVRAGGAIAVLTRHTNLSMLRTPSRLELTYLATADALSRMVANGEFPH
                     PTAPTGLRRGAPRVGDGVIRLDTEGVITYASPNSVSALHRLGYVGEILGTSLAELVTS
                     VLHDTSPVDEGLPLVLTGRQPWRTEVTSAGQVISMRAIPLSESGQRIGAIVLARDISE
                     LRRREQELMTKDATIREIHHRVKNNLQSVAALLRLQARRMPDAQGRAALEEAVRRVGT
                     IALVHDTLSHGLDETVEFDQVADVTIRAVVEVAAGGRSVAVRRTGSFGRVRAEDATSL
                     AMVVSELVQNAAEHGVASQGGTINLEVKRKEGKDETDVLTVTVTDDGVGLPGGRLPAG
                     AGLGTQIVQSLVQDLRGKISWGPAEPQGTRVRFTARLR"
     gene            complement(10772..>11825)
                     /locus_tag="PLK64_05165"
     CDS             complement(10772..>11825)
                     /locus_tag="PLK64_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HPV79162.1"
                     /translation="DGRGLTSPEFSVLVAYSKLDLKAALLPTALADDPWFAGTLAEYF
                     PTRLRNEHANQLDQHPLKREIIINSVVNSLINRGGITFAFRAAEETGATPEQVARAFI
                     VCREVFDLAGFSAAVESLDATLPAKVQTVLYLEFRRLLDRALRWFLANRPGTIDVTEE
                     IARFLAPVQEHAALVPEVLQGSERRRLDRRAAELAALGVPEALAMRTASLLDVFSMLD
                     IVEIAQATGTPSQDVLRVYYHASERFGIDDMLFRVSQLARDDRWDSAARGALRDDLYA
                     VLDGITRNVLATSDPKADAKSRFNQWAKANQEALDRARSGLQAILRLESANIAALSVA
                     LRTLRAFVKTATPTDG"
BASE COUNT         1949 a         4131 c         3999 g         1746 t
ORIGIN      
        1 cgtcagcacg cggccgaccg ggggcgtatc gcatggcgag gcgggtaggt gcgccgaagc
       61 ggcccaaggt agcctggacc acggccggac cgggcgtggg tccgcctggc ctcacacccc
      121 ctgcacagcc gtccccgacg tcaacgtcga cgcaccgaaa gaggcgaggc acccgtgcca
      181 gaccaggcga agagcgacaa ccccatgggc gcattcgggc ccaacgagtg gctcgtcgac
      241 gagctctacg accagtggct caatgacaag gactcggtcg atcgagcctg gtgggagttc
      301 ttcgaggact accgtcccgg ccacccgtcg ggccccctcg gtaacggcca ccgcgccgcg
      361 ccagcagcgc cggcacccgc gcccagcctg cctgtgtcgg cgcccacccc agcacccgcg
      421 ccggccccgt ccacaccggt cgttgccggt tccgccggca ccccgggcgc tcccgtcgca
      481 ccgggtgccg cgccactggc agcccccgcg ccgaccgcgc ccatgccgcg cgacatcccc
      541 gcgccgaagt ccacgccccc gccgtcggag gccgagatcc gaccgctgcg cggacccgcc
      601 gcgcgcgtcg tggtcaacat gcagtcctcg ctcgaggtgc ccacggcgac ctcggtgcgc
      661 acggtgccgg ccaagttgct catcgacaac cgggtggtca tcaacaacca cctggcccgc
      721 tcgcgaggcg gcaaggtctc gttcacgcac atcatcggct acgccctggt caaggccctc
      781 aaggccctgc cggcgatgaa ccacgccttc ggcgagcagg acggcaagcc cgtcgtcatc
      841 gccccggcac acgtcaacct cgggttggcc atcgacctgg ccaagcccga cggcacccgg
      901 caactgctcg tcccgtccat caagtcggcc gagctcatgg acttcgtgca cttctggtcg
      961 gcctatgaag acatggtcaa gaaggcgcgc ggcggcaagc tcaccgtcga tgacttcgcg
     1021 gggacgacga tctcgctgac caacccgggg acgatcggca cggtccactc ggtgcctcgg
     1081 ctcatgcagg gtcagggcac gatcgtcggc gtcggcgcca tggagtaccc ggccgagtgg
     1141 cagggggcct ccaacgagac cctcaaccgc aacgccgtct ccaagatcct cacgctcacc
     1201 tcgacctatg accaccggat catccagggt gcccagagcg gtgacttcct gcggatcgtc
     1261 caccacctgc tgctcggcgg tgaccggttc tacgacgaga tcttcgagag cctgcgcatc
     1321 ccctacgagc cgatccgctg ggtgcaggac gtcacggcca cccacgacga cgacatcaac
     1381 aaggtcgccc gggttcagga gatcatccac gcgttccggg tgcggggcca cctcatggcc
     1441 gacaccgacc cgctggaata ccgccagcgc cgccaccccg acctcgacat caccgagcac
     1501 ggcctgaccc tctgggacct cgaccggcac ttcgcgaccg gcgggttcgg cggcaagccg
     1561 ttcctcaagc tgcgtcagat cctcgggatc ctgcgcgact cctactgccg caccgtcggc
     1621 atcgaataca tgcacatcca ggaccccacg cagcgcaagt ggatccagga ccagtgcgag
     1681 gtcgtccacg agaagcccac ccgcgacgag cagatgcgca tcctgcgtcg gctcaacgcc
     1741 gcggaggcct tcgagacctt cctgcagacc aagttcgtcg gccagaagcg cttctccctg
     1801 gagggcggcg agtccgcgat cgcgctgctg gaccggatcc tctcggctgc ggccgagggg
     1861 ggcatggagg aggtgtgcat cggtatgccg caccgcggcc ggctcaacgt cctggccaac
     1921 atcgcgggca agtcgccggc gcagatcttc cgtgagttcg aaggtaaggc tgacccgaag
     1981 tcggtccagg gctccggcga cgtgaagtac cacctgggca ccgagggcac cttcacggcc
     2041 gagaacggcg cctcgacgaa ggtctacctc gcggccaacc cctcgcacct cgaggcggtc
     2101 aacccggtcc tggaaggcat cgtccgcgcc aagcaggacc gcctcgacct cggcggggac
     2161 gcctacaccg tgttgccgat cctcatgcac ggcgacgcag cgttcgccgg gcagggcgtt
     2221 gtggccgaga ccttgcagca gtccgagctg cgcggttacc gcaccggcgg aacgatccac
     2281 gtcatcgtca acaaccaggt cggcttcacc acggcgccct cggcgtcgcg ctcgtcggtc
     2341 tactccaccg acgtcgcccg gatgatccag gcgccgatct tccacgtcaa cggtgacgac
     2401 cccgaggcct gcgtccgggt ggccgagctc gccttcgagt tccgccagaa gttcaagaag
     2461 gacgtcgtcg tcgacatggt gtgttaccgc cgccgcgggc acaacgaggg cgacgacccg
     2521 tcgatgaccc agccgctcat gtacaacctc atcgaagcca agcgctcggt ccgcaagctc
     2581 tacaccgagt cactcatcgg tcgtggcgac atctcggtcg aggacgcctc ggccgcgttg
     2641 cgcgactacc agcagcagtt ggagcgggtg ttcctcgaga cccgcgaagc ccaggccgca
     2701 ccggcagcca gccagacggc atacgtcgac tccacctcgg acgtcgcggg ccacggcggc
     2761 ctggagaagc ccacggcgca ggcgctcgac gacgtgtcgc gcccacgcga ctggcgccag
     2821 acggccatct cggtcgacac cctcaacacc ctgggcgacg cgtgggtccg cccgccggac
     2881 ggtttcaccg tccaccccaa gctcaaggcc ctcatggacc gtcgggcagc gatggtccgc
     2941 gaaggcggga tcgactgggg catgggcgaa ttgctcgccc tcgggtcgct gctcatggag
     3001 ggcacgccgg tccggctggc cggtcaggac agccagcgcg gcaccttcgt ccagcggcac
     3061 gcggtgctca tcgacaagat caccggacgg gagtggaccc cgctgcacca cctgcaagag
     3121 ggtcaggcga agttctgggt ctacaactcg ctgctgtcgg agtacgccgc gatgggcttc
     3181 gagtacggct actccgtgga gcggccggat gcgctcgtcc tgtgggaggc gcagttcggc
     3241 gacttcgcca acggggcgca gaccatcgtc gatgagttca tctcctcctc ggagcagaag
     3301 tgggcgcagc gttcgtcggt cgtgctgctg ctcccccacg gctacgaagg ccaaggaccg
     3361 gaccactcgt cggcccgcat cgagcgcttc ctgcagttgt tcgccgagga caacatgacg
     3421 atcgcctacc cctcgacccc ggcgtcccac ttccacctgt tgcgccgtca ggcctacgcc
     3481 cgcccgcgtc gcccgctcat cgtgttcgcc cccaagtcga tgctgcggct caaggccgcc
     3541 gcgtcgatgc cgcaggactt cacgaccggc ggcttccgcc cggtgctgcc agacacccgt
     3601 cacctcgacg gcggcgcggt cacccgcgtg ctcatggcgt cgggcaaggt cgtctgggag
     3661 ctcgaggccg agcgtgacaa actcggtgac acgagcaccg ccctgctgcg catcgagcag
     3721 ttggccccgg ctccggccga ggagctcgcc gcagccctac ggggatatcc caacgccgag
     3781 ctcgtgtggg tccaggacga gccgaagaac cagggtgcct ggccgttcat ggcgctcaac
     3841 ctgccggagc tgttggccga gcagggcgag aaccgcccgc tgcgcgtcat cgcccgcaag
     3901 gcctcggcct cgccggcctc tgggtcacac aagaagcacg agctcgagca ggctgcgttg
     3961 ctcggggcgg ccttcgcgcg ctgacccgac ccgacaccct tggtatgccg cgcgctcaag
     4021 tgagcgcgcg gcatacccct gtgtggtgga cgccactgtg ggctgggacg acgactgtgc
     4081 gtggggtcac ggtcgtgcgg tggacacgat gcggaggccg cgatagacct gagggagacc
     4141 cgttcgagga ggaaccgtgg cccgcgttgt cgcccagccc taccgttcac cgtggatgac
     4201 cgacgatctg gacgagttgt ccagtctggc tcggacgttc ttcgcgcggg aggtgacgcc
     4261 gaacgccgag cggttcgccg agcagcacca ggtcgaccgc gaaacctggc ttaaggcggg
     4321 tggggcaggg ctgctcgggc tgagcgtgcc ggaggagttc gggggtggcg gcggcatgtt
     4381 cgcgcacgag gcggtcgtcc tccaggagca gggttgggcc ggggacgacg cgctcggcct
     4441 gcacatccac agtgggatcg tcccccagta catcgtcgag ttcggcacgc tcgagcagaa
     4501 gcgtcggtgg ctgcccaaac tggcctcggg cgagtgggtc ggggcgatcg cgatgactga
     4561 gccgggcacg ggctcagacc tgcaggcggt gcgcacgacc gcccgccgcg atggcgacga
     4621 ctacgtcgtc aacggcagca agaccttcat caccaatgcc acgcacgccg acctcgtcat
     4681 catcgtctgc cgcaccggcg cgccgggcgc caaaggcatc tcgctgctcg tcgccgaggt
     4741 caacgacctg cccggcttca cccgcggccg ggtcctggcc aagatcggcc agcacgggca
     4801 agacacccgt gagctcgcct tctcggacat gcgcgtcccg gcggcgaacc tcctcggcga
     4861 ggagggtcac ggcttcggcc aactcatgga gcaactgccg caggagcgcc tcgttgtcgc
     4921 ggtgggcgcg gtcgcagcgg cggagtatgc cgtgcgtctc gccacggcat actcgaagga
     4981 acgcgaggcg ttcgggcgca agatcctcga cttccagcac acccggttcg tgctctccga
     5041 ggtctccacc gacgcgctgg ccgcgcgcac cttcctcgat cactgcatca ccgagcacct
     5101 ggctgggcgc ttggacgcgg tgctcgcctc gcggatcaag tactgggcca ccgacgtgca
     5161 gtgctcagtc atcgaccgct gtctgcaggt cttcggcggc tacggctaca tgatggagta
     5221 cccgatcgct cggctctatg ccgccgcccg cgtgcagaag atctacgccg gcaccaacga
     5281 gatcatgaag gaactcatct cgcgatcatt gtgacccggc ggcgttggga cgggcagtaa
     5341 cccggtaacg gaaggcgcaa gtgctcgcgc cgtgacgagt gcgcgcgacc cggcgcgata
     5401 gcctccgagg gtgtacttca ccgaccgtgg catcgaggaa ctcgacgcgc gccggggcga
     5461 cgaagaagtg accttctcgt ggctggccga gcatctgcgc accttcgtcg acctgcaccc
     5521 ggagttcgaa acccctgtcg agcggctggc gacctacctc gcccgactcg acgacgacga
     5581 ggagtgaacc actcgtgacc gaagaccatg tgaccggcca cgacggcccg gcctacccaa
     5641 gtgcgacgga cggcgaggcg gcacccgagt tcaccccgag tgcgtccttc accgtcgatg
     5701 acgatggccg acgggacatc gggttgtgct tcgtcggtga ctcgttcacc gtcgggttcg
     5761 gcgaccccaa ggcgctgggg tgggtgggcc gggtcgtgag tcggacgcac caccccgacg
     5821 cggaggtcac ggcatacaac ctcggggtcc gcgggcagga ctccggtgac gtgatggcgc
     5881 ggtggcgcgg ggagtgcatg ccgcgctggg tcggccgcgg cgagcggcgc ctggtcgtgg
     5941 cggtcggcgc caacgacctc gggcaagggt tgacgaccgc gcgctcgcgt ctcaacctcg
     6001 ccaacatcct cgacgatgcc tccgcgaagg ggatcgcgac gttcgtcgtc gggctgaccc
     6061 cgtcgctcga tcccgacttg aacgccagga tcggggtgct cgcgcaggcg caggccgacg
     6121 tgtgctcccg ccgtggcgtc acctatgtcg actgctaccg gcccttggtg gcgcacgagc
     6181 agtgggctgc cgacctcgcg gccggcgacg gggtgcaccc ggggcaggcg ggctacgggc
     6241 tgatcgcctg gctggtgctg cacgccggct ggaacgcgtg gatgcaactg ggctgacagt
     6301 cgaccgtctg gttcccgtgg cccttgcgat gcggactatc gcgatataac gtgtctctct
     6361 aagacgcgat atagcgcgtg catcggagtc gaggagacac catggccgaa caatggttag
     6421 tcgacggtcc gcgcgtcatc gacgtgggca gtgatcgcga gcgggtcgag aaggtcgtcg
     6481 ttggcatcat cggtggccgc gtgaacgtcg tgacgcatga cgacgggtac ggcgcgcgag
     6541 tcgaagtctc gaacgtcgag ggcctgccac tgcgggtctc ctgggatggc cgaaccctca
     6601 aggtgatgca caccaaggat ggtgggagca cggtgctgga caccctgcga cgcctcgtcg
     6661 agcaggtcag cggtcagcac gccgatgtca gcatctcggt gcccgcatcc gcccgcgcga
     6721 ccgtgtcgac cgtgaccgca cccatcgtcg tcagcgggct gcgccgcggc ctggccgcca
     6781 acaccgtgtc cggcgagatg acgatcagcg acgtgcaggg ccgcagcacg atcaacaccg
     6841 tcagcggggc caccgagtgc gccgacctcg tcggcgacgc caagatcaac tccgtctctg
     6901 gctcggtgac ggtgcagagc agcgatatcc ctcgggctcg catcaacacc gtctccgggg
     6961 aggtcgcgct cgacttggtt aacacccact tggacgtcca gtccaactcg gtcacgggcg
     7021 acgtcaccat ccgcgcgccc ttctcggggt atgccgtcac cgccaccagc gcgaccggcc
     7081 aggtcatcgt cgatggccag tccctgcacg gtgccagcag cgggagtatg ccgcccggcg
     7141 gctggggcga ctgcgcaccg ggcggttcga gcgggcgcgg aacgctgcgc agcggcgacg
     7201 agtcactgac cctgcgcgcc aattcggtgt cgggcgacgt catcctgctg cgggccgccg
     7261 caccagcggc cggtccgtcc ggcagtgggg atgctgggcc acaggacagg tctcccgaag
     7321 tgtctcccgg cggaccgcag gatgcgcccg cgcgcgacga ggtgcgggaa tcgtcaattg
     7381 acgacgaggg gcaggacacc ccgtgagccc cgtcttcgcc cacggacaac tgcgccttta
     7441 cctcctggtc ctgctcgctg aggcaccgcg tcatgggtac gaggtgatcc aggacctgga
     7501 gacccggttt ggcgggctct acacacccag cgccggcacg gtctatcccc ggttggccaa
     7561 actcgaagag gaagggctcg tcgagcggac cgacgagggc cgcaaggcga cctatcggat
     7621 caccgaggcg gggctcgccg aggtgcgcgc ccgtcatcgg gaggtggaag acctccaagc
     7681 cgacctcgac caatcggtgc gtcgcctcgc cgagcaggtc cgagccaccg tccagggtcg
     7741 ctcggccgat ctgcgggcgg agttgaaagc ggctgcgcgc gaagcgcgaa cgacggcgcg
     7801 tccggccgga tcgacctcgg ggacgggctg gccacaggga tcgagcccgg gatggggcca
     7861 gtcgcccacg gaccgaaggg caggcgcggg agcgtatggc gggacgggcg acaggtcagg
     7921 ctcacggtct gactccgggt caggcgccgg gtctggccgc gcgtctggcg gcgggtcggg
     7981 ggccgggacg ttcgccgggg cggattggac ggacgtcgag cgcgcggcgt ccgaggtgcg
     8041 cgagcaggcc cgccgggcct ggcgccagca tgggctcacg gctgagcaga ccgccgagat
     8101 cgtggagatc ctggccgaca cgtcccgtcg gatcgcgcaa gtgctgcgta tgccgcgcga
     8161 ctgaccgacc cggtcgatca gtcgctaccg gaaccgcgag ccactgtggc tgctccgatc
     8221 gacagcacga tggcgacaga cagcgagagt gtgacgagcc actggccggc ctgcgccatg
     8281 ctgacgacga ccgggatgag cagggcgtgc cagacgacgg tgggagtgcc tggccacccg
     8341 gagcgaccga gccagagccg ggccaacaag gccatcccgg cggcgaaggc caggatgagg
     8401 accgattcgg tgatgacccg agcggtcccg gtgccgcccc cccgggcgag ttcaaccaaa
     8461 ccgtagatcc cgaatccggc catggccagc gcctcaatcg cgcagatgat cgccgccacc
     8521 cgccgcgccg aactaaccct cggacggaag ccaccagtac tcacggcccg agcctaacgg
     8581 cgcggcggcg ggcggtcgca cccccttcga ctgctggaag cgcagcgttg taggcccctg
     8641 gatgctccga ttcgtaccgt ttggcaggaa gaaagcgccg ttcgggaaca aacgtgatgg
     8701 atgtcccgtt gccgaaaggt agtcctttgg accacaatgt cgtgttggcc tcccagagtt
     8761 gcgggtgcca tgacggcttg cgggttccgt gggggatacc cgcatccacg gtcgtctcag
     8821 cattgcactc atcatttggg ggaccccgac ggtcctggac agaacctgca tcatcgacat
     8881 ctgcttgacc ccagacggca cgacctcgaa ctaggagcac gatccacatg gactggcgcg
     8941 accgcgctgc ctgcctcgac gaggaccccg agctgttctt ccccattggg aacaccgggc
     9001 ccgcgattct gcagatcgaa gaggccaaag cggtctgccg tcgttgcgaa gtcctcgaca
     9061 cctgcctgcg atgggcgctg gagtccggtc aggacgccgg cgtgtggggc gggctgtccg
     9121 aggacgagcg ccgcgcgctc aagcggcgca acgcccgcgc ccgtcgcgcc ggctagttcc
     9181 accgccgccg ccgcgtttat cacctgcgca cggtcacctg cgcatggtca cctgcgcgct
     9241 gtcgccgatg gcttggctgc accgccagga tcgagcggtt aacgtaaccg tgccgtgaag
     9301 cggacccggg ttccctgcgg ttcggcaggt ccccacgaga tcttcccccg cagatcctga
     9361 acgagggatt ggacgatctg cgtgcccagg ccggccccgg ccggaagtcg tccaccaggc
     9421 aaacccacac cgtcatcggt gacggtcacg gtaagaacgt cggtctcgtc cttgccctcc
     9481 ttgcgcttga cctccaggtt gatcgtcccg ccctgcgacg cgaccccgtg ttcggcggcg
     9541 ttctggacca actcgctgac aaccatggcc agcgaggtgg cgtcctctgc ccgaacccgc
     9601 ccgaacgacc cggtccgtcg cacggcgacc gaccgcccgc cggcagcgac ctcgacgacc
     9661 gcgcggatcg tgacatcagc gacctggtcg aactcgacgg tctcgtccag gccatggctg
     9721 agggtgtcgt ggacgagcgc aatcgtgccg acccgtcgca cggcttcttc gagggccgcc
     9781 cgcccctgcg catcaggcat ccggcgggcc tgcagacgaa gcagggcggc caccgattgg
     9841 agattgttct tcacccggtg gtggatctcc cggatggtcg cgtccttcgt catcaattcc
     9901 tgctcacggc ggcgcaactc gctgatgtcg cgcgccagca cgatggcccc gatccgctgc
     9961 cccgactcgc tgagcgggat cgcccgcatc gagatcacct gaccggcact ggtcacctcg
    10021 gtccgccacg gttggcggcc cgtgaggacg agcggcagac cttcgtcgac cggcgaggtg
    10081 tcgtgcaaga ccgacgtgac gagttcggcg agcgacgtgc cgaggatctc ccccacatag
    10141 cccagccggt gcagggccga caccgagttg gggctcgcgt aggtgatgac cccctcggtg
    10201 tccagccgga tcactccgtc gccgacgcgg ggagcccctc ggcgcaggcc cgtcggcgca
    10261 gtcgggtggg ggaactcccc attggcgacc atccgcgaga gcgcgtcagc agtggccagg
    10321 tacgtgagct ccaaccggct cggggtgcgc agcatcgaca ggttggtatg ccgcgtcagc
    10381 accgcgatcg cgccgccggc ccgcacgacg gggatcgact cctcacgcac ggggtcatcg
    10441 gggtcccact cgctgtcgcg ctcgcggacg atccggcgct gcaggtatgc cgcctccagc
    10501 aactccaccc ggctggtgcc ggcaacccgg cctacctgat cgcggaagta gaccgtttgc
    10561 cccgtcgtcg gccggatgtg ggcgacggcg agccaacgat caggggaact gcgcacccac
    10621 aggacgaggt cggcaaagga caggtctgag agcaactgcc agtccgagac gagcagccgc
    10681 agccactcct ggtccacctg tgggagggcg gcatgctcct ggatcagctc ggagagggtc
    10741 gccacgccgc gactctagcg ccgagcccag gtcagccgtc cgtcggtgtg gccgtcttga
    10801 cgaaagcacg cagcgttcgc aacgccaccg agagggccgc aatgttggcc gactccagac
    10861 gcaggatcgc ttgcagcccc gaccgggccc ggtcgagcgc ttcctggttg gccttggccc
    10921 attggttgaa gcgcgacttg gcgtcggcct tcgggtcgct cgtcgcgagg acgttgcggg
    10981 tgatcccgtc gagcacggca tacaggtcat cgcgcaacgc accacgcgcg gcggaatccc
    11041 accggtcgtc ccgcgcgagc tgggacaccc ggaagagcat gtcgtcgatc ccgaaccgtt
    11101 cggacgcgtg gtagtagacc cgcaggacgt cctgcgacgg tgtgcccgtg gcctgcgcga
    11161 tctcgacgat gtcgagcatc gagaagacgt cgaggagtga cgcggtgcgc atcgccagcg
    11221 cctccggaac accgagtgcg gccaactcgg ccgcccggcg gtccagacgg cgacgctcgg
    11281 acccctggag cacctcgggg acgagagcgg cgtgctcctg gaccggcgcg aggaaccgag
    11341 caatctcctc ggtcacgtcg atggtgccgg gccggttggc caggaaccat cgcaaggcac
    11401 ggtccagcag tcgacggaac tccaggtaga gcaccgtctg caccttggcc ggcagggtcg
    11461 cgtcgaggga ctcgacggca gccgagaacc cggccaggtc aaacacctcg cggcaaacga
    11521 tgaacgcgcg agcgacctgc tccggcgttg ctccggtctc ctcggccgcc cggaaggcga
    11581 acgtgatgcc gccccggttg atcaacgagt tgaccaccga gttgatgatg atctcgcgct
    11641 tgagcgggtg ttggtcgagc tggttggcat gttcgttgcg cagccgggtc gggaagtact
    11701 ccgccagggt gcccgcgaac cacgggtcgt cagccagcgc ggtgggcagc agagccgcct
    11761 tgaggtcgag cttggaatag gcgacgagga ccgagaactc cggcgaggtc aggccccgcc
    11821 cgtcg
//
LOCUS       DAOMZY010000061        11773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5102_length_11773_cov_5.833163, whole genome shotgun sequence.
ACCESSION   DAOMZY010000061 DAOMZY010000000
VERSION     DAOMZY010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 11773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11773
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5102_length_11773_cov_5.833163"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="PLK64_05170"
     CDS             complement(<1..700)
                     /locus_tag="PLK64_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HPV79163.1"
                     /translation="MSSRQAPRSREVSAGAVRPAGALPRVAVVGAAAVPGSRILDTIA
                     RAARASGDGRPPVAIDRDRGRVATAEWRLVDPTDPALVRALRDVDIAVWVAAETDLES
                     ALRVRPTERRDLVVRTAQTLVTAAAAAGVSRLVVVTSAKVYGATPDNPVPLPEDSELN
                     GVRDGGVVGDLLAVEEVIQDARRVHPGLSITVVRPAALVGPGIDTVVTRHFEAPRLLT
                     VKGANPAWQFCHVDD"
     gene            763..2295
                     /locus_tag="PLK64_05175"
     CDS             763..2295
                     /locus_tag="PLK64_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HPV79164.1"
                     /translation="MSEPNDDAAAVPDDEASVPSGSQRPTGGPQDRPGATGANQPGGI
                     PDLEPLVRELFGPDATLPPELTELLGALRADPTGSPLAGMLQAQFRSLFAPASTADRV
                     ATATDLARKVVAAKGDPSVGPVQRRAVEEAVAVARLWLDPVTALDSPTGPALALSGAE
                     WVQATTPRWYALVEPVSDGVTAAAGAALRAQVEQLGGLSGGLEGVPELQALLGAIPGG
                     AGLASGAGLWAQIGPALDHLSAGMFGAQLGQAVGTLAGDVLSGTEVGLPLMDSGAVAL
                     LPEQVSEFARGLEVDEGQVRLYLAVREAARVRLFAEVPWLAAQLEAAVRDYGRNVAID
                     TDAIEEAVRSVELTDLSNLSAMQEAFSQAQLFGRRTTPEQDAALIRLEATLALVEGWV
                     DLVTDQAVAAHLPQAQALGEAVRRRRAGGPAQRAFAGLVGLELRPRRLRDARNLWAAL
                     EDAGGVELRDGSWAHPDLAPAAADLDDPLGYVDRRQHPAAPDEMDAELDRLLSGQDPD
                     AL"
     gene            2292..2897
                     /locus_tag="PLK64_05180"
     CDS             2292..2897
                     /locus_tag="PLK64_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPV79165.1"
                     /translation="MSVATAYRHLHSDAVRLLAGWTPPDARQERLRADYLTHLGAHPD
                     GVAKAGPPAHLTASALVFDSRLERVLLTHHGKARVWLQLGGHLEPEDASLYAAAQREI
                     REESGLARVHVIPVIAQLDRHTLVGAFGHCREHLDVRFAAVVPAGSEDAVSASAESID
                     LRWWPLEALPEPTSAEVGALAKACLAVLHAAPSVSPEPSRT"
     gene            complement(3038..3562)
                     /locus_tag="PLK64_05185"
     CDS             complement(3038..3562)
                     /locus_tag="PLK64_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPV79166.1"
                     /translation="MISDQATKRLLLLRHAKAEQTFGVLDHERELTDRGRRDAHAVGE
                     WLAAQEIDADLVICSTALRTRQTWDHVAAGGARAEAITFDSAIYHGGPTGVLQALVEG
                     AGTAATVVIVGHAPTMPMLAEALTAGRGSAQAHSELAAGFPTTGLAVLRYAGHWADLG
                     QGTAELEAFFIGRG"
     gene            complement(3601..4527)
                     /locus_tag="PLK64_05190"
     CDS             complement(3601..4527)
                     /locus_tag="PLK64_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HPV79167.1"
                     /translation="MRTVTLPGQSWITDVGPVDGLAMRAWDLSDDLDDPSVRVVVPPM
                     GTTERLARLAALPGLEVVQVFTAGYDHVLAHLPSGVTLCNAAGVHDASTAEFAVALML
                     AAQRGIPEFARAQERGEWIGQRAWPALGDRRVLIVGYGRIGRALAARLAPFEVSLTAV
                     ASRPRAGDDVVERVHGFDELPWLLPHHDIVVLLTPLTDTTRGLADAAFLASMPDGALV
                     VNVARGQVLDTAALLAETATGRLRAALDVTDPEPLPPDHPLWHAPGVLVAPHVGGAGD
                     TFRPRMVRLLREQLTRYAAGAPLLHVVAGPGA"
     gene            4689..5561
                     /locus_tag="PLK64_05195"
     CDS             4689..5561
                     /locus_tag="PLK64_05195"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HPV79168.1"
                     /translation="MSDIDQLLTQIPLAAIAQELGVDESQAAASSRTALEALLGGLQA
                     NASDPAGALSLGQALGTHDGSLLGADLGDIDQEDGSAIVHNIFGGNTSDVIAQLGGVK
                     GSGGSGLIAKLLPILAPFVLAWLGQKLRGQGGMGDILGQVLGGAAGGAGGSSSGAGGG
                     GLGDILGQVLGGAAGGGSSSGAGGGGLGDILGQVLGGSGTSAGSADSADSGLGLPEQP
                     TGPLIPTDGSDMSDVTDGSSNRSTGAQDNPMGGVLGDILGQVLGGGSSSGSGSSSSPN
                     ITDILGGLLGGGKR"
     gene            complement(5643..7202)
                     /gene="gatB"
                     /locus_tag="PLK64_05200"
     CDS             complement(5643..7202)
                     /gene="gatB"
                     /locus_tag="PLK64_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HPV79169.1"
                     /translation="MSEHTSDDLALDGGPEAVLTWDETIAAYDPVLGLEVHVELGTAT
                     KMFCGCPTEFGAEPNSQVCPVCLGLPGSLPVVNEKGVESAIRIGLALNCTIAPWSRFA
                     RKNYFYPDMPKNFQTSQYDEPIAFNGWLDVELEDGSAYRVEIERAHMEEDTGKSLHVG
                     GATGRIHGADYSLVDYNRAGIPLIEIVTKPLTGFGDRAPEVAKAYVATLRDLLRALGV
                     SDVKMEQGSLRCDANVSLRPRKKKPEKQAEVPFGTRTETKNVNSLRSVERAVRYEMTR
                     HAAVLAGGGSILQETRHWHEDTGVTTSGREKSDAEDYRYFPEPDLVPIAPTADSIEAL
                     RATLPEPPVQRRRRLQGEWGYSDLEMRDVINAGAIEIIEATVAAGATPAGARKWWSGE
                     IARRANLDGRELTDMGITPRAVAELDALVQGGRLNDSMARQVLDGVLAGEGNPGAVAD
                     ARGLALVSDDGALGAAVDEVIAANPDLVEKIRSGKVQAAGALIGQVMKAMAGQADAAR
                     VRELIMERVGS"
     gene            complement(7199..8692)
                     /gene="gatA"
                     /locus_tag="PLK64_05205"
     CDS             complement(7199..8692)
                     /gene="gatA"
                     /locus_tag="PLK64_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HPV79170.1"
                     /translation="MADSLARREISAVELTQAHLDRIAAVDGSVHSYLHVDETGVLAQ
                     ARAVDDARARNEDLHVMAGVPIAVKDVVATEGLPTTCGSRILSGWIPPYDATLVTKLK
                     AAGMPILGKTNMDEFAMGSSTEHSAYGPSRNPWDLKRIPGGSGGGSSSVLASFQAPLA
                     IGTDTGGSIRQPASVTGTVGTKPTYGGVSRYGLVALASSLDQAGPCARTVLDTALLHA
                     VIGGYDPRDSTSIHAPVPEVVRAAQSADVSGMRIGVVKQLSGEGYQAGVQARFNEAVA
                     LLVDAGAAVVEVDCPNFQYALAAYYLILPSEASSNLARFDAMRYGLRALPEGVDDPSA
                     EQVMAATREVGFGAEVKRRIILGTYALSSGYYDAYYGQAQKVRRLIAQDFDRAFEVAD
                     VLVSPTAPTTAFKIGEKLDDPLAMYLNDVATIPANLAGIPGMSLPSGLATEDGLPTGF
                     QILAPATKDERLYAVGSALERELVSRWGGHLLEKAPTLATVGAEVAR"
     gene            complement(8816..9112)
                     /gene="gatC"
                     /locus_tag="PLK64_05210"
     CDS             complement(8816..9112)
                     /gene="gatC"
                     /locus_tag="PLK64_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HPV79171.1"
                     /translation="MSSLSRDDVAHLAMLARIELTDAELDRMLGELSVILDAVALVQQ
                     APTEGVEPMSHPLPLVNVTRPDEVRPGLTSAQALSGAPEADQQRFSVPRILSDD"
     gene            complement(9178..11334)
                     /gene="ligA"
                     /locus_tag="PLK64_05215"
     CDS             complement(9178..11334)
                     /gene="ligA"
                     /locus_tag="PLK64_05215"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPV79172.1"
                     /translation="MRQDDAVSSSEAPAGAPDVPSEVRHAWTELAETIRAHQFAYHVQ
                     DSPTVSDGEYDALIRDLAALEESHPSLRTPDSPTQQVGAPSFSTEFQAVDHLERMLSL
                     DNAFSPEELAAWAARVERDAGGPGGAAYRFLCELKVDGLAINLLYERGRLTRALTRGD
                     GRTGEDVTHNVRTIRGIPAVLTPTAQAPVPELVEVRGEVFFPVEQFEELNASLVEAGR
                     APFANPRNAAAGSLRQKDPRVTASRPLRMLVHGLGARRGFDVARQSEAYAVLHDWGLP
                     TSPHVRVVDTVAEVQAFIDHFGEHRHSVEHEIDGVVVKVDEVAVQRSMGSTSRAPRWA
                     IAYKYPPEEVNTVLRAIEVGVGRTGRVTPFGVMDPVVVSGSTVSNATLHNGWEVARKD
                     VRIGDTVVLRKAGDVIPEIVAPVLALRPADAVPWEMPTHCPACGSLLAAEKEGDKDIR
                     CPNARSCPAQLRERLFGLASRGALDIEALGWEGAAALLDAGVIDDEGMLFALTAAEIA
                     TVPLYTRLAKKTDPEADVIDGRVLSANGEKLLDNLAKAKKQPLWRVLVALSIRHVGPT
                     AARALATHFGSMEGIRAASAEELSGVDGVGGVIAESVRDWFDAPDNTWHRAIVEAWAA
                     AGVRMVDAVDASVARTLDGATVVVTGSLEGFTRDGAKEAIIARGGKAAGSVSKNTDWV
                     IVGPGAGSKEAKARDLGLRILDEAGFVRLLETGSGD"
     gene            complement(11371..>11773)
                     /locus_tag="PLK64_05220"
     CDS             complement(11371..>11773)
                     /locus_tag="PLK64_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HPV79173.1"
                     /translation="TVLAAAGEHRTIVHCCAAYPPLPLLRRSGAGGLSVDTTLITPRG
                     WEGLAVAVEDGVRLHAGCVPTAPEAVVDPVAVADHLASAWSRVGMPPAHLDDLVVTPT
                     CGLAGLTPSAAIERQRACLQVAAELVTRSRG"
BASE COUNT         1662 a         4193 c         4235 g         1683 t
ORIGIN      
        1 ggtcatcgac gtggcagaac tgccatgcgg gattggcacc cttgaccgtc aagagccggg
       61 gtgcctcgaa gtgtcgggtg acgacggtgt cgatgcccgg cccgacgagg gccgccggcc
      121 tcaccaccgt gatggacagg ccgggatgga cccgacgggc gtcctggatg acctcctcga
      181 cggccaacag gtcaccgacg acgccaccgt cacggacccc gttgagctcc gagtcttccg
      241 ggagcggcac cgggttgtcg ggcgtcgcgc cgtagacctt ggccgacgtg acgacgacca
      301 gccggctcac cccagctgcc gcggcggcgg tcacgagggt ctgggccgtg cgcacgacga
      361 ggtcccggcg ttcggtcggg cgcacccgca gggcgctctc tagatcggtc tcggcggcca
      421 cccagacggc gatgtcgacg tcccgcagcg cccggaccag ggcggggtcg gtcggatcga
      481 ccagacgcca ttcggccgtg gcgacccggc cacggtcccg atcgatggcc acgggcggac
      541 gcccgtcgcc ggaggctcgg gctgcgcggg cgatcgtgtc gaggatccgg gaacccggaa
      601 cggcggcggc accgacgacg gcaacgcgcg gcaaggcacc cgcggggcgc accgcccctg
      661 cggagacctc ccgagaccgt ggagcctgcc gggaactcac gccgccctcc tgtggttgtc
      721 ttgggtgcca tcttctcagc ccctgcttag gagcacgatt tcatgtccga gccgaacgac
      781 gacgccgcag ccgtccccga tgacgaggcc tccgttccgt ccggttcgca gcgccccacg
      841 ggcggtccgc aggaccggcc cggggcgacc ggcgccaacc agccgggcgg catacctgat
      901 ctcgaacccc tcgtgcgcga gctcttcggt cccgacgcga cccttcctcc cgagctgacc
      961 gagttgctcg gcgcgttgcg cgccgacccc accggatccc ccctcgccgg catgctccag
     1021 gctcagttcc gctcgctctt cgccccggcc tcgacggccg atcgggtcgc gaccgcgacc
     1081 gacctggccc gcaaggtggt cgccgccaag ggcgacccgt cggtcgggcc ggtgcagcgc
     1141 cgtgccgtcg aggaggccgt ggcggtcgcc cggctctggc tcgacccggt caccgcgctc
     1201 gactccccca ccggaccggc gctggcgctc agcggcgccg agtgggtgca ggcgacgacc
     1261 ccgcgctggt acgccctcgt tgaaccggtc tccgacgggg tcacggctgc ggccggggcg
     1321 gcgctgcgag cgcaggtcga gcagttgggt gggctctccg gaggtctcga aggggttccc
     1381 gagttgcagg cgttgctggg agcaatcccc ggcggcgcag gcctggcctc aggtgctggg
     1441 ctgtgggctc agatcggccc cgccctcgac cacctctcgg ccggaatgtt cggcgcccag
     1501 ctcggccagg cggtcggcac gcttgccggt gacgtcctct ccggcaccga ggtcgggctc
     1561 cccctcatgg acagcggggc cgtcgcgctg ctgcctgagc aggtctccga gttcgctcgt
     1621 ggtctggagg tcgacgaggg tcaggtccgc ctctacctcg ccgtgcgtga ggcggcccgg
     1681 gtccggctct tcgccgaagt cccttggctg gcagctcaat tggaggcagc ggtacgcgac
     1741 tacgggcgca acgtcgcgat cgacaccgac gcgatcgagg aggccgtccg gtccgtcgag
     1801 ctcaccgatc tgagcaacct gtcggccatg caggaggcct tctcgcaggc ccagctcttc
     1861 ggtcgacgca cgacccctga gcaggacgcc gccctcatcc gcttggaggc caccctcgcg
     1921 ctcgtcgagg gctgggtcga cctcgtgacc gaccaagccg tcgccgccca cctgccccag
     1981 gctcaggcgc tcggcgaggc ggtcaggcga cgtcgcgccg gagggcccgc ccagcgcgcc
     2041 ttcgccggtc tggtcggcct ggagttgcgg ccccgacggt tgcgggacgc gcgcaacctg
     2101 tgggccgcat tggaggacgc cggcggcgtc gagttgcgcg atgggtcttg ggctcacccg
     2161 gacctggccc ccgccgcggc cgacctcgac gacccgctgg gctacgtcga ccgccgtcaa
     2221 caccccgccg cacccgacga gatggacgcc gagttggacc ggctcctgtc gggccaggac
     2281 ccggacgcgt tgtgagcgtc gcgacggcat accggcatct gcactccgac gcggtccgtc
     2341 tgctggcggg gtggaccccg cccgatgccc gacaggagcg cctgcgggcc gactacctca
     2401 cccacctggg tgcccacccc gacggtgtcg ccaaggccgg gccgcctgcc cacctcaccg
     2461 cgagcgccct ggtcttcgac tcgcggcttg agcgggtcct gcttacccac catggcaagg
     2521 cccgggtctg gttgcagttg ggtggtcacc tcgagcccga agacgccagc ctgtatgccg
     2581 ccgcgcaacg cgagatccgc gaggagagcg ggctggccag ggtccacgtg atcccggtga
     2641 tcgcgcaact ggaccggcat accctcgtcg gagccttcgg gcactgccgg gagcatttgg
     2701 acgtccggtt cgcggcggtc gtgccggccg gctcggagga tgccgtgtca gccagcgccg
     2761 agtccatcga cctgcgttgg tggcccctgg aggcgttgcc cgagcccacc agcgccgagg
     2821 tcggtgcctt ggccaaggcc tgcctcgccg tgctgcacgc tgcgccgtcg gtgtccccgg
     2881 agccctcgcg cacctgatcc aaggcccgca cccgtgccgg acagtggtgc cgggcctggg
     2941 gccgtccatg aggccgtgac agctggcgtg atcacgccag ctgtcgcggc ttcatgagcg
     3001 gcttcatccc cgggcagcgg cgccgtccac caatcggtca gccgcgaccg atgaagaagg
     3061 cctccaactc ggcggttccc tgacccaagt cggcccagtg accggcatac cgcagcacgg
     3121 cgaggcccgt cgtcgggaag ccggctgcga gctcggaatg ggcctgcgcc gagccacgcc
     3181 cggccgtgag cgcctcggcg agcatcggca tcgtcggggc atgaccgacg atgacgacgg
     3241 tggcagcggt gcccgcgcct tcgacgagag cctgcagcac cccggtcggt ccgccgtgat
     3301 agatcgccga gtcgaaggtg atcgcctccg ctcgcgcgcc acccgccgcg acgtggtccc
     3361 aggtctggcg ggtgcgcaaa gccgtggagc agatgaccag gtcggcgtcg atctcctggg
     3421 ctgccagcca ctcgccgacg gcgtgggcgt cccggcggcc gcggtcggtg agctcccgct
     3481 cgtggtcgag gacaccgaag gtttgctcgg ccttggcatg gcgcaggagc agcagtcgct
     3541 tggtcgcttg gtcgctgatc accccctgag gatgcccggt ccggcccgcg ctcgccaccc
     3601 tcaggccccc ggtccggcga cgacgtgcag caggggtgcg cccgcggcat accgggtcag
     3661 ttgctcacgc aacagacgca ccatccgcgg ccggaaggtg tcgcccgccc cgccgacgtg
     3721 cggcgcgacg aggacgcccg gggcgtgcca cagcggatgg tcgggcggga gcggctcggg
     3781 gtcggtgacg tccagcgcag cccgcagccg gcccgtggcg gtctcggcca gaagcgcggc
     3841 agtgtcgagc acctggccgc gggcgacgtt gacgaccagg gcgccgtcgg gcatcgacgc
     3901 gaggaacgcg gcatccgcaa gccctcgggt tgtgtcggtg agcggcgtga gcagcacgac
     3961 gatgtcgtga tgcggcagca gccacgggag ctcgtcgaac ccgtggaccc gctcgaccac
     4021 gtcgtccccg gcacgcggac gcgaggccac cgcggtgagg ctcacctcga acggcgcgag
     4081 ccgagcggcc agggcgcgcc cgatccgccc gtagccgacg atgagcaccc gccggtcgcc
     4141 caacgcgggc caggcacgct gcccgatcca ctcgccgcgt tcctgagccc gcgcgaattc
     4201 cggtatgccg cgttgcgcgg cgagcatgag cgcgaccgcg aactccgcgg tggaggcgtc
     4261 gtggacgccc gcggcattgc acaaggtcac ccccgacggc aggtgcgcca agacgtggtc
     4321 gtagccggcg gtgaacacct ggacgacctc gagccccgga agcgcagcca agcgagccag
     4381 ccgctctgtc gtgcccatcg gcggcacgac gacgcgcacc gacggatcgt cgaggtcgtc
     4441 ggacaagtcc caggcccgca tggccagccc gtccacgggc cccacgtcgg tgatccagga
     4501 ctgcccgggc agggtcacgg ttcgcacggt ccgacgctac cgcccggacg ccgatgggcc
     4561 agcccgcgac cggcgggcgc gagtgacccg ccgacggtcg gtgcgggccc gctgggggat
     4621 ccgccgacag gcgtcgatcg ttagggtgag caggcggccg caggggctgc atccacggga
     4681 gggaacccat gtccgacatc gaccaactgc tcacgcagat tccactggct gcgatcgccc
     4741 aggaactggg ggtcgacgag tcccaggccg cggcatcctc gcgcacggcg cttgaggcgc
     4801 tcctcggcgg gctgcaggcc aacgcgagcg acccggcggg cgcgctgtcc ctcggccagg
     4861 cgctcggcac ccacgacggc agcctgctcg gcgccgacct cggggacatc gaccaggagg
     4921 acggctcggc gatcgtccac aacatcttcg gaggaaacac ctccgacgtc atcgcccagc
     4981 tcggcggcgt caaggggtcc ggcggctccg gcctcatcgc caagttgctg ccgatcctgg
     5041 ccccgttcgt gctggcctgg ctgggccaga agctgcgcgg tcagggcggg atgggcgaca
     5101 tccttggtca ggtcctcggc ggagcggccg gcggcgccgg tggcagttcg tccggtgcgg
     5161 gcggcggcgg tctcggtgac atcctcggtc aggtcctcgg cggagccgcc ggcggcggca
     5221 gctcgtccgg tgcgggcggc ggcggtctcg gtgacatcct cggtcaggtc ctcggcggct
     5281 ccggcacgtc ggccggctcg gctgattcgg ccgattcggg tttggggctg cccgaacagc
     5341 ccaccggtcc cctcatcccg accgacggca gcgacatgtc cgacgtcacc gacggctcga
     5401 gcaaccgctc caccggcgcg caggacaacc cgatgggtgg cgtgctcggc gacatcctcg
     5461 gtcaggtcct cggcggcggt agctcgtcag gctccgggtc cagcagcagc cccaacatca
     5521 ccgacatcct cggcgggctg ctcggcggcg gtaagcgctg acccaccacc cacggcgggt
     5581 atgccgcgcg gcacgtccgc gcgcggcata cccgctggtg tgtgtggggt ggagcgtgcg
     5641 tcctagctgc cgacccgctc catgatgagc tcgcggaccc gcgcggcgtc ggcctgacca
     5701 gccatcgcct tcatgacctg accgatgagg gcgccggcgg cctggacctt cccgctgcgg
     5761 atcttctcga cgaggtcggg gttggcggcg atgacctcat cgaccgcggc acccagcgcg
     5821 ccgtcgtcgg acacgagcgc tagaccacgg gcatcggcga ccgcaccggg gttgccctcg
     5881 ccggcgagca caccgtcgag cacctggcgg gccatcgagt cgttgagtcg gccgccctgc
     5941 acgagcgcgt ccaactcggc gacagcacgt ggcgtgatgc ccatgtcggt gagctcgcgg
     6001 ccgtcgaggt tggcgcgccg ggcgatctcg cccgaccacc acttgcgagc accggccggg
     6061 gtcgcaccgg cggccacggt ggcctcgatg atctcgatgg caccggcgtt gatgacgtcg
     6121 cgcatctcca ggtcggagta gccccactcc ccctgcagcc gtcggcgacg ctgcaccggc
     6181 ggctcgggaa gggtcgcccg caatgcctcg atcgagtcgg ccgtcggagc gatggggacc
     6241 aggtcgggct cggggaagta gcggtagtcc tcggcgtcgg acttctcgcg gccactggtc
     6301 gtgaccccgg tgtcctcgtg ccagtgccgg gtctcctgca ggatcgaacc gccaccggcc
     6361 agaaccgctg catgccgggt catctcgtaa cgaacagccc gctccaccga ccgcaacgag
     6421 ttgacgttct tggtctcggt gcgggtgccg aaggggacct cggcctgctt ctcgggcttc
     6481 ttcttgcgcg ggcgaagcga gacgttggcg tcacaccgca gcgacccctg ctccatcttg
     6541 acgtcggaca cgccaagggc ccgcaagagg tcccgcaggg tcgcaacgta ggccttcgcc
     6601 acctcgggcg cgcgatcacc gaagccggtc aacggcttgg tgacgatctc gatgagcggg
     6661 atgccggctc ggttgtagtc gaccagcgag tagtcggcac cgtggatgcg cccggtggcg
     6721 ccgccgacgt gcagcgactt gccggtgtct tcctccatgt gggcccgctc gatctcgacc
     6781 cggtaggcgc tgccgtcctc caactcgacg tcgagccacc cgttgaaggc gatcggctcg
     6841 tcgtactggc tggtctggaa gttcttgggc atgtccggat agaagtagtt cttccgggcg
     6901 aaccggctcc acggcgcgat ggtgcagttg agcgccagcc cgatccggat ggccgactcg
     6961 acgcccttct cgttgaccac cggcagcgac cccggcagcc cgagacagac cgggcacacc
     7021 tgcgagttgg gctcggcgcc gaactcggtc ggacaaccgc agaacatctt ggtcgcagtg
     7081 cccaactcga cgtggacctc caacccgagc accgggtcgt atgccgcgat cgtctcgtcc
     7141 cacgtcagca cggcctcggg gccgccgtcg agggccaggt catcactcgt gtgctcgctc
     7201 atcgagccac ctccgcgccc acggtcgcca aggttggcgc cttctccaac aagtgcccgc
     7261 cccaccggct gacgagctca cgctccagag ccgaccccac ggcatacagg cgctcgtcct
     7321 tcgttgccgg ggcgaggatc tggaacccgg tcgggagccc gtcctcggtc gcgagaccgg
     7381 acggcagcga catccccggg atgccggcga ggttggccgg gatcgtcgcg acgtcgttga
     7441 gatacatggc caacgggtcg tcgagcttct cgccgatctt gaacgcggtc gttggcgcgg
     7501 tcggggagac gaggacgtcg gccacctcga aagcgcggtc gaagtcctgg gcgatgaggc
     7561 gacggacctt ctgggcctga ccgtagtaag cgtcgtagta gcccgacgag agggcatagg
     7621 tgcccaagat gatccggcgc ttgacctcgg cgccgaaccc gacctcgcgg gtggctgcca
     7681 tgacctgctc ggcgctcggg tcatcgactc cctcgggcaa ggcccgcagg ccatagcgca
     7741 tcgcgtcgaa gcgagccagg ttggaggacg cctcggaggg caggatgagg tagtaggcgg
     7801 ccagcgcgta ctggaagttg gggcagtcga cctcgaccac cgctgcgccg gcatcgacga
     7861 gcagggcgac cgcctcgttg aagcgggcct gcacccccgc ctggtagccc tcgccggaca
     7921 gctgcttgac gaccccgatc cgcatcccgc tgacgtcagc gctctgcgcc gcccgcacga
     7981 cctcgggcac cggggcgtga atggacgtcg agtcgcgcgg gtcgtaaccc ccgatgacgg
     8041 cgtgcagcag cgcggtgtcc aggacggtcc gggcgcacgg cccggcctgg tcgaggctgg
     8101 aggccaacgc gacgaggccg tagcgcgaga ccccgccgta ggtcggcttg gtgccgaccg
     8161 tccccgtcac cgacgccggc tgacggatcg agccgccggt gtcggtgccg atcgccaacg
     8221 gggcctggaa ggacgccaac accgacgacg agccgccgcc cgagccgccg gggatgcgct
     8281 tgaggtccca cgggttgcgg ctggggccat aggccgagtg ctccgtcgag gagcccatcg
     8341 cgaactcgtc catgttcgtc ttaccgagga tcggcatccc ggcggccttg agcttggtca
     8401 cgagagtcgc gtcgtacggc gggatccacc ccgagaggat ccggctgcca cacgtcgtcg
     8461 gcagcccttc ggtcgcgacg acgtccttga ccgcgatcgg cacgccggcc atcacgtgca
     8521 ggtcctcgtt gcgggcgcgt gcgtcatcga cggcccgcgc ctgtgccagc acgccggtct
     8581 cgtcgacgtg caggtaggag tggacggagc catcgacagc cgcgatgcgg tccaggtggg
     8641 cctgggtgag ctcgacggcg ctgatctccc gacgggccag agagtcggcc atccgggcgg
     8701 cgctccagcg ggtgagatcg ttggtcacgg tggtgcctta cgttggggag cgggtcggga
     8761 agctgcaggt atgccgcgcg cctgggccgc gaggcggcat accagaggcg gtgggtcagt
     8821 cgtcggagag gatccggggg acggagaagc gctgctgatc ggcctcggga gcccccgaga
     8881 gggcctgcgc ggaggtgagc ccggggcgga cctcgtcggg cctggtgacg ttgaccaggg
     8941 gcaaggggtg cgacatcggc tcgacgcctt cggtgggcgc ctgctggacc agtgcgacgg
     9001 cgtcgaggat gaccgacagc tcgccgagca tccggtcaag ctcggcgtcg gtcagctcga
     9061 tccgcgccag catggccaga tgggcgacat cgtcgcggga cagggatgac atggctccat
     9121 cctattggcg ctgcttgtgg acccttgcgg gacgttcggc ttagcccacc ctggccgtta
     9181 gtcgccagac ccggtctcca ggagccggac gaaccctgcc tcatcgagga tgcgcagccc
     9241 caagtcgcgg gccttggcct ccttggaccc ggcacccggc ccgacgatca cccaatcggt
     9301 gttcttcgag accgatccgg ccgccttgcc accgcgggcg atgatcgcct ccttcgcgcc
     9361 gtcgcgggtg aagccttcga gggagccggt gacgacgacg gtagcgccgt ccagggtgcg
     9421 ggccaccgag gcatcgaccg cgtcgaccat ccggaccccg gccgctgccc aggcctcgac
     9481 gatggcccgg tgccaggtgt tgtccggggc gtcgaaccaa tcgcgcaccg actcggcgat
     9541 cacgccgccc accccgtcga cgcccgagag ttcctcagcg ctcgcggccc ggatgccctc
     9601 catcgagccg aagtgggtgg cgagggcgcg ggccgcggtg gggccgacat gcctgatcga
     9661 cagggcgacg agcacccgcc acagcggctg cttcttggcc ttggcgaggt tgtcgagcag
     9721 tttctcgccg ttggccgaca ggactcggcc atcgatcacg tcggcctcgg ggtcggtctt
     9781 cttggccagc cgggtataga gcggcacagt ggcgatctcg gcggctgtga gcgcgaacag
     9841 catcccctcg tcgtcaatga caccagcgtc cagcagtgca gccgccccct cccagccgag
     9901 ggcctcgatg tccagggcgc cccgggaggc caggccgaag agtcgctcgc gcaactgggc
     9961 cgggcaggac cgggcgttgg ggcaacggat gtccttgtcg ccctccttct cggccgccag
    10021 caggctcccg caggcggggc agtgcgtcgg catctcccac gggacggcgt cggccggacg
    10081 caacgccagg acgggggcaa cgatctcggg gatgacgtcg ccggccttgc gcagcacgac
    10141 ggtgtcaccg atccggacgt ccttgcgggc gacctcccag ccgttgtgca aggtggcgtt
    10201 ggagacggtc gagccgctga cgaccacggg atccatgacg ccgaacggag tcacccgacc
    10261 ggtgcgaccg accccgacct cgatggcccg aagaaccgtg ttgacctctt ccggcgggta
    10321 tttgtaagcg atcgcccagc gcggcgcacg cgaggtcgag cccatcgacc gttggacggc
    10381 gacctcgtcg accttgacga ccaccccgtc gatctcgtgc tcgaccgagt gtcggtgctc
    10441 tccgaagtgg tcgatgaacg cctggacctc ggcgacggtg tcgacgaccc gcacgtgcgg
    10501 ggaggtgggc agcccccagt cgtgcagcac ggcatacgcc tcgctttggc gggccacgtc
    10561 gaagccgcga cgagccccga gcccgtggac gagcatccgc agggggcggc tcgcggtgac
    10621 ccgcgggtcc ttctggcgca gcgaaccggc agcggcgttg cgggggttgg cgaacggcgc
    10681 gcggccggcc tcgacgaggc tcgcgttgag ctcctcgaac tgctcgacgg ggaagaagac
    10741 ctcgccgcgc acctcgacga gctcagggac cggcgcctgg gcggtgggcg tgagcacggc
    10801 ggggatgccg cggatcgtgc ggacattgtg ggtgacgtcc tcgccggtgc gcccgtcgcc
    10861 gcgggtgagc gcgcgggtga ggcggccgcg ctcgtagagg aggttgatcg ccaggccgtc
    10921 gaccttcagc tcgcagagga accggtatgc cgcgccgccg ggccccccgg cgtcccgctc
    10981 cacccgcgcg gcccaggccg ccagctcctc gggcgagaag gcgttgtcga ggctgagcat
    11041 ccgctcgagg tggtcaaccg cctggaactc ggtcgagaag ctcggcgccc ccacctgttg
    11101 ggtcgggctg tcgggcgtgc gcaggctggg gtgggactcc tccagggccg cgaggtcgcg
    11161 gatgagcgcg tcgtactccc cgtcggacac ggtcggggaa tcctggacgt gataggcgaa
    11221 ctggtgggct cggatcgttt cggccagctc ggtccaggca tgccggacct cggatggcac
    11281 gtcgggggcg cctgcgggcg cttcggagct ggacacggcg tcatcctgcc gcatcgcgcc
    11341 gacaatccct cgacggcggg gtgcggtcgc tcagcctcgg gagcgggtga cgagttccgc
    11401 ggccacctgc aggcaggcgc gttggcgttc gatggcggcg gacggggtca accccgccag
    11461 cccacaggtc ggggtgacga ccaggtcgtc caggtgagcg ggcggcatac cgacgcgcga
    11521 ccaggccgag gcgaggtggt cggcgacggc gacggggtcc accaccgcct cgggtgcggt
    11581 gggcacacaa ccggcgtgca ggcgaacccc gtcctcgacc gccacggcca agccctccca
    11641 gccgcgcggc gtgatcagcg tcgtgtcgac cgagagcccg cccgcgccac ttcggcgcag
    11701 caggggcagc ggagggtatg ccgcgcagca atgcacgatc gtccggtgct cgcccgccgc
    11761 cgccagcacc gtc
//
LOCUS       DAOMZY010000062        11680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5154_length_11680_cov_6.252903, whole genome shotgun sequence.
ACCESSION   DAOMZY010000062 DAOMZY010000000
VERSION     DAOMZY010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 11680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11680
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5154_length_11680_cov_6.252903"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="PLK64_05225"
     CDS             complement(<1..817)
                     /locus_tag="PLK64_05225"
                     /inference="COORDINATES: protein motif:HMM:NF033745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HPV79174.1"
                     /translation="MTAALQVQLDPGATGPTQRRRGRHRARRVWVDRLIPVLLMLIGA
                     VVLAYPVVATFYNNYKQSEFAREYAAKVATADPAALNAEFAAATRYNTALNPKLLKDP
                     WDEASSALNADYRDYLSQLDLLEAMGRIRIPALDVDLPVLHGTSDDTLAKGVGHLFGS
                     SLPVGGPSTHAVLTGHSSLANATLFDHLPDLSVGDQFYLEVLGRTLAYRVDKISVVLP
                     NQLDDLAVTSGADQVTLVTCTPYAINSHRLLVRGVRVPYTQADGSAPGQATSTM"
     gene            complement(997..2700)
                     /locus_tag="PLK64_05230"
     CDS             complement(997..2700)
                     /locus_tag="PLK64_05230"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HPV79175.1"
                     /translation="MKSLRMAAAAFAAVALAGLGLIAPTSAMALPSPIIDPTATVTLN
                     IAKFLDNTTGQPNNGTQQTVSGTPLEGVDFDVYKFTTIDLTTNAGWAASIVLQDHTLT
                     AGEIADGFVTVNSVNYPFTLVTTVTTDAAGLATFTQAAGAGLYLVNENLGTSTTTPDA
                     STITPSAPFLVTLPMTDPVNLTSWMYDVYVYPKNQADSILKTVTDGNLGTTNQNAYTV
                     GQNLTYHLASTIQATDSNGDGSINGADLSYYYVGDNLSTYVDAQSVTVSVGGTPLIGC
                     DATAGAGTDCDYYYWLDYAAAAGDEVKIIMSTSGLDKIVAAKVFDAAAVVTTDIVATV
                     TSVPSTGIIPNKGEFIPSEGWWIGQGGTDLTTPGTTTPPTTVPPTTTTPGIPSNEVVS
                     KYGDMQFIKYDAKTSAPLTGAEFTVSRDLNNDDACAPAEMIVANQIGSPVTTDASGAV
                     LFTDLQLSNFYNNATQTDLHTYCLVETKAPVGYNLNAQPIPFTVTVEGDVTNLAYTVG
                     TADAQEVQVANEPSNLGNNLPLTGGAGVAAVSIVGLLLVGGGLGYYLVGARRREIEQD
                     V"
     gene            complement(3349..6036)
                     /gene="ppdK"
                     /locus_tag="PLK64_05235"
     CDS             complement(3349..6036)
                     /gene="ppdK"
                     /locus_tag="PLK64_05235"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017824352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPV79176.1"
                     /translation="MTTRYVFDFSEGDKEQKALLGGKGANLAEMVKIGLPVPPGFTIT
                     TEACKAYLTKGKAPAELAAEVTAHLATVEQAMGRTLGDPINPLLVSVRSGAAFSMPGM
                     METVLNIGLNDDSVLGLAGMAGERFAWDSYRRLLQMFGKTVLGIDSEVFEARIEQVKH
                     ARRIVEDTELTAADLAALCGSFKTAIFNATGERFPQDPRQQLDAAVEAVFRSWSGDRA
                     VLYRRQERIPNDLGTAVNIQTMVFGNMSDDSGTGVCFTRDPSTGDVGVYGDFLMNAQG
                     EDVVAGIRNARPLHELEGESPAAYRQLLDNMSALEAHYHDLCDIEFTIERGTLWMLQT
                     RVGKRTAEAAFRIAGQLIDEGVITEEEALTRVNGAQLSQLMFPRFDASATSALLTRGV
                     AASPGAAVGKIVFSSIAAKEWADRGEDVILVRTETNPDDLVGMIASKGILTSRGGKTS
                     HAAVVARGMGTTCVCGADELVIDSTHKTITIRGRAGENLKEGDLISIDGGTGQVFRGA
                     VPVMPSAIVEYFEGNIDPEAPETSDTVKAVHRIMTIADKVRRLRVRANADTPEDAARA
                     VRFGAEGIGLCRTEHMFLGDRRELVERLILAETDETRVAALEALRPLQRQDFQEIFAA
                     MGERPVTVRLIDPPLHEFLPNIVEMATKVAIAEERGTATDADRTMLKAIERLHESNPM
                     FGLRGVRLGIVTKGLFEMQVRAILEAFQLARKSGSVGTAEIMVPLIDTYQELELVRTQ
                     ALKIAREKGELVGPGENVMIGTMMELPRACLTAGQIAESADFFSFGTNDLTQTTWGFS
                     RDDVEGSFFSTYLEKGIFPVSPFETIDEDGVGRLVTFAVQQGRETKPGLKTGVCGEHG
                     GDPESVHFFHKVGLDYVSCSPFRVPVARLEAGRAAVMNR"
     gene            complement(6361..10275)
                     /locus_tag="PLK64_05240"
     CDS             complement(6361..10275)
                     /locus_tag="PLK64_05240"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPV79177.1"
                     /translation="MLDVNFFDELRIGLATADDIRQWSHGEVKKPETINYRTLKPEKE
                     GLFCERIFGPTRDWECSCGKYKRVRFKGIICERCGVEVTRAKVRRERMGHIELAAPVT
                     HIWYFKGVPSRLGYLLDLAPKDLEKVIYFAAYMITAVDVDQRHADLPSLEAQIQVERK
                     ELENRRDVDIDDRAKKLEADLAELEAEGAKADIKRKVRDGAEREMGSIRRRADQQIER
                     LEQVWDRFRNLKVQDLEGDEILYREMRDRFGLYFEGGMGAAAIQKRLESFDLDAEAIL
                     LREIIDTGKGQRKTRALKRLKVVNAFQTTTNSATAMVLDCVPVIPPDLRPMVQLDGGR
                     FATSDLNDLYRRVINRNNRLKRLLDLGAPEIIVNNEKRMLQEAVDSLFDNGRRGRPVT
                     GPGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKQMALE
                     LFKPFVMKRLVDLDHAQNIKSAKRMVERSRPVVWDVLEEVITEHPVLLNRAPTLHRLG
                     IQAFEPQLVEGKAIQIHPLVCTAFNADFDGDQMAVHVPLSAEAQAEARILMLSSNNIL
                     KPADGRPVTMPTQDMIIGLYHLTCETTTVYAAESGEGAEALRSFSTPAEARMAFDSGQ
                     IELGSLVRIRLFDTVPPAGFVLPEGVEAGEDGRVPVITLETTLGRALFNEALPMNYPF
                     VNEPVDKKRLSMIVNDLAERYSKVQVAASLDALKEAGFHWATRSGTTVAISDVITPPR
                     KAEIIEEYEARAAKVQKLYDRGAMTDNERREDLIEIWNEATNEVAKEMIANTPRTNTI
                     FRMVNSGARGNWMQLRQIAGMKGLVSNPKGEIIPRPIKANFREGLSVLEFFISTHGSR
                     KGLADTALRTADSGYLTRRLVDVSQDVIIREEDCGTERGLKVPIATIDPQGRRVGHED
                     VETSVYARTLAEDVEVDGTVLATAGIDLGDVVIDRLFEAGVDEVRVRSVLTCDSKVGT
                     CALCYGRSLAAGKLVDIGEAVGIIAAQSIGEPGTQLTMRTFHIGGVAGDDITQGLPRV
                     VELFEARTPKGVASIAEATGRIRIEDSDKARRIVLIPDDGAEEHSYPVSKRARLLVAD
                     GDHIEVGHQLFQGAIDPKQVLRILGPRAVQMHLVDEVQHVYRQQGVSIHDKHIEVIVR
                     QMLRRVTVIEAGEADLIPGDLVERGAFEAENRRVVTDGGKPASGRPELMGITKASLAT
                     ESWLSAASFQETTRVLTQAAMEAKSDPLRGLKENVILGKLIPAGTGLARYRNIEVEPT
                     EEAKAAMYSMPNYDSYDYSHFGGGTGEAVRLDDLNLDDYGRL"
     gene            complement(10377..>11680)
                     /locus_tag="PLK64_05245"
     CDS             complement(10377..>11680)
                     /locus_tag="PLK64_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPV79178.1"
                     /translation="LVQDDVLSSIHIEEHEVDARDTKLGPEEITRDIPNVSEEVLADL
                     DERGIIRIGAEVRDGDLLVGKVTPKGETELTPEERLLRAIFGEKAREVRDTSLKVPHG
                     ETGTVIGVKVFDKDDGDDLPPGVNQLVRVYVANKRKITDGDKLAGRHGNKGVISKILP
                     IEDMPFLEDGTAVDVVLNPMGVPGRMNVGQILELHLGWAASRGWEIEGTPDWARAIPA
                     DNHVAEPGARVASPVFDGAREDVITGLLDSTRKTRDGVRLIDGSGKTRLFDGRSGEPF
                     PEPVSVGYMYILKLHHLVDDKIHARSTGPYSMITQQPLGGKAQFGGQRFGEMEVWALE
                     AYGAAYTLQELLTIKSDDVPGRVKVYEAIVKGENIPDSGIPESFKVLLKEMQSLCLNV
                     EVLSSDGQAIEMKESDDDVFRAAEELGIDLSRREPSSVEEV"
BASE COUNT         1811 a         3780 c         3996 g         2093 t
ORIGIN      
        1 ccatggtgga cgtcgcctgc ccaggcgctg agccgtcggc ttgcgtgtag ggcacccgaa
       61 cccctcgcac cagcagccga tggctgttga tggcgtatgg cgtacaggtg accagggtca
      121 cttggtccgc gccgctggtc acggcgaggt cgtcgagctg attgggcagc acgaccgaga
      181 tcttgtcgac ccgataggcc agggtgcgac caaggacctc gagatagaac tgatcgccga
      241 cgctcaagtc cggaagatgg tcaaagagcg tggcgttcgc caaagaactg tgtccggtga
      301 gcacggcgtg tgtgctcggt ccgcccaccg gcagcgaact gccgaacagg tggccgacac
      361 ccttggccag cgtgtcatcg ctggtgccgt gcaggaccgg aagatcgacg tccagcgccg
      421 gaatccgaat cctgcccatc gcctcgagca gatcgagctg gctcaggtag tcgcggtagt
      481 ccgcgttgag cgcgctgctc gcttcgtccc aggggtcctt caagagcttc gggttcagtg
      541 ccgtgttgta gcgcgtcgcc gcggcgaact ccgcgttcaa cgcggccgga tcggccgttg
      601 ccaccttggc ggcgtactca cgggcgaact cggactgttt gtagttgttg tagaacgttg
      661 cgacgaccgg gtaggccagg acgaccgctc cgatcagcat cagaagcacc gggatgagcc
      721 ggtcgaccca caccctgcgg gcgcgatgcc ggccccgacg gcgctgggtt ggcccagtcg
      781 ctccggggtc gagctgcacc tgcagggcgg cggtcattcg tctcctcctg gatcgggtgg
      841 tggggagggg gttgacgacc tccccaccac cctgatgggt gcggcatgga aggcgagggg
      901 gggttccttc gcatgccgct gtcaaacggg cggcgctttc acaccgtggt tctcagctca
      961 ccaatccggt ctgggaggac cgaggttgga gacctgtcag acgtcctgct cgatctcgcg
     1021 gcgacgggcg ccgacgaggt agtagccgag gccaccaccg acgagcagga gaccgacgat
     1081 gctcaccgca gccacgcccg caccaccggt gagggggagg ttgttgccga ggttggacgg
     1141 ctcgttggcc acctgaacct cctgagcgtc agcggttccg accgtgtagg ccaggttcgt
     1201 cacgtcaccc tcgaccgtca ccgtgaaggg gatcggctgg gcgttgaggt tgtagccgac
     1261 gggcgccttc gtctcgacca agcagtaggt gtgcaggtcg gtctgggtgg cgttgttgta
     1321 gaagttcgac agctgcaggt cggtgaagag cacggcacca ctcgcgtcgg tggtcaccgg
     1381 cgacccgatc tggttggcca caatcatctc ggccggggcg cacgcgtcgt cgttgttgag
     1441 gtcacgggag acggtgaact cagcaccggt gaggggagcc gacgtcttgg cgtcgtactt
     1501 gatgaactgc atgtcgccgt acttcgagac gacctcgttc gacgggatgc cgggagtcgt
     1561 ggtcgtcggc ggcacggtcg tcggcggggt cgtcgtcccc ggggtggtca ggtcggtccc
     1621 accctggccg atccaccagc cctcgctggg gatgaactcg cccttgttgg ggatgatgcc
     1681 cgtcgagggg accgaggtga cggtcgcaac gatgtcggtc gtgacgacag ccgccgcgtc
     1741 gaaaaccttg gccgcgacga tcttgtccag gccggaggtc gacatgatga tcttgacctc
     1801 gtcgccggcg gcggcggcgt agtcgagcca gtagtagtag tcacagtcgg tgcctgcccc
     1861 ggccgtcgcg tcgcaaccga tgagcggggt gcctccgacg gagacggtca ccgactgcgc
     1921 gtccacgtag gtcgacaggt tgtcaccgac gtagtagtag ctcaggtccg caccgttgat
     1981 cgagccatcg ccgttgctgt cggtggcctg aatcgtcgag gcgaggtggt aggtgaggtt
     2041 ctgaccgacg gtgtaggcgt tctggttcgt cgttccgagg ttgccgtcag tgaccgtctt
     2101 gaggatcgag tcggcctggt tcttcgggta gacgtagacg tcgtacatcc agctcgtcag
     2161 gttgaccggg tcggtcatcg gcagcgtgac gaggaacggt gccgaggggg tgatggtcga
     2221 cgcgtcaggg gtcgtggtgg acgtcccgag gttctcgttg accaggtaga ggccagcgcc
     2281 ggcggcctgc gtgaacgtcg ccaggcccgc tgcgtcagtg gtcacggtcg tgaccagggt
     2341 gaacgggtaa ttcacgctgt tgaccgtgac gaaaccgtcg gcgatctcac ctgcggtgag
     2401 ggtgtggtcc tgcaacacga tcgaggcggc ccaaccggca ttggtcgtca ggtcgatggt
     2461 ggtgaacttg tagacatcga agtcgacacc ctccagcggg gtgcccgaca ccgtctgctg
     2521 ggtgccgttg ttcggctggc ccgtcgtgtt gtcgaggaac ttggcgatgt tcagggtgac
     2581 cgtggcggtc gggtcgatga tcgggcttgg cagcgccatg gccgacgtcg gggcgatgag
     2641 cccgagccct gccagggcca cggccgcaaa ggcagccgct gccattctga gtgatttcat
     2701 gcgcattcct tgacactcct ccaaagagag ttttccgttg ttgttctagg gagttcgcgg
     2761 ctgcgacctg tcccgtcatc ctccgagagc gaccacgtgg gtggcccggt tctggaagtc
     2821 ctcgtccctt cacccgtcct cgttgagagc ccggcggctg cctcacggca cccaggaacc
     2881 ctcctcagcg gaggctcatc ggctgctccc aaccgatgag acaccaggcg gggtcggacg
     2941 cggacgtcga agagtggcat accctcgggg gtatgccgta ggcacggcag agcaaggctc
     3001 agcacggctg actccaacgc atcgtgtttt gaatcccgca gtcagccgtc tcatccgggc
     3061 gacccctgcg cgtccccccg gacggcgtca agactgttcc cgtagatgcg cccccacagc
     3121 gccgaccaac tgcgtcaatg ttaacccaga tatacccgcg ttgtccagca cgacgcgtgc
     3181 ttgctcaagg acgcctcaag cgcttgcacg tattcacagc cgttcactat tgcttccccc
     3241 tagcaaccgg cggccactcg ggcctgaagt gcgggagtca tcgtccccga tacacagaga
     3301 acgggcgcga accccgcagg ggttcacgcc cgttcttggc gaagtcggtc agcgattcat
     3361 gacggccgca cgcccggcct cgagacgcgc caccggcacg cggaacggcg agcaggagac
     3421 gtagtccaga cccaccttgt ggaagaagtg caccgattcg gggtcgccgc cgtgctcacc
     3481 gcagacgccc gtcttgagcc caggcttggt ctcccggccc tgctgaacgg cgaaggtcac
     3541 caaccgcccg acgccatcct catcgatggt ctcgaaggga gacaccggga agatgccctt
     3601 ctcgagatag gtcgagaaga acgacccctc gacgtcgtcc cggctgaagc cccacgtcgt
     3661 ctgggtgagg tcgttggtcc cgaaggagaa gaagtcagcc gactcggcga tctgaccggc
     3721 cgtcaggcag gcccgcggca actccatcat cgtgccgatc atgacgttct cacccggccc
     3781 gaccaactca cccttctcgc gggcgatctt cagcgcctgg gtgcgcacca actcgagctc
     3841 ctggtaggtg tcgatgagcg gcaccatgat ctcggccgtc ccgaccgaac cactcttgcg
     3901 ggccagttgg aaggcctcga ggatcgcccg gacctgcatc tcgaacaggc ccttggtgac
     3961 gatgcccagc cgcaccccgc gcaacccgaa catcgggttg gactcgtgca gccgctcgat
     4021 ggccttgagc atcgtgcggt cggcgtcggt ggccgtgccc cgctcctcgg cgatcgcgac
     4081 cttggtcgcc atctcgacga tgttgggcag gaactcgtgc aacggcgggt cgatgagccg
     4141 cacggtgacc ggccgctccc ccatcgccgc gaagatctcc tggaagtctt gacgctgcag
     4201 cggacgcagc gcttcaaggg ccgccacccg ggtctcgtcg gtctcggcga ggatgagtcg
     4261 ctcgaccaac tcgcgccggt cgccgaggaa catgtgctcg gtgcggcata ggccgatgcc
     4321 ttcggcgccg aagcggaccg cgcgcgctgc gtcttcgggc gtgtcggcgt tggcgcgcac
     4381 ccgcagccgg cgcaccttgt cggcgatcgt catgatgcgg tgcaccgcct tgacggtgtc
     4441 tgacgtttcg ggtgcctcgg ggtcgatgtt gccctcgaag tactcgacga tggcgctcgg
     4501 catgaccggc acggcgccgc ggaacacctg gccggtgccc ccgtcgatcg agatgaggtc
     4561 gccttccttg aggttctcac cagcccgacc gcggatggtg atcgtcttgt gcgtgctgtc
     4621 gatcaccaac tcgtcggcgc cacagacaca ggtcgtgccc atgccacgag ccacaacagc
     4681 ggcgtgcgag gtcttgccgc cccgcgaggt gaggatgccc ttgctggcga tcatgcccac
     4741 gaggtcgtca gggttggtct cggtgcggac gaggatgacg tcctcgccac ggtcggccca
     4801 ctccttggcg gcgatcgagc tgaagacgat cttgcccacc gccgcgccgg ggctggccgc
     4861 gacgcccctg gtgagcaggg ccgaggtcgc cgacgcgtcg aaccgcggga acatcagctg
     4921 ggacagctgg gcgccgttca cccgggtgag cgcttcttcc tcggtgatga cgccctcatc
     4981 gatgagctgg ccggcgatcc ggaaggccgc ctccgcggtc cgcttgccga ctcgggtctg
     5041 caacatccac agggtgccgc gctcgatggt gaactcgatg tcgcacaggt cgtggtagtg
     5101 cgcctccagg gcgctcatgt tgtccagcaa ttgccggtat gccgcgggcg actcgccctc
     5161 gagctcgtgc agcgggcggg cgttgcggat gccggcgacg acgtcctcgc cctgggcgtt
     5221 catgaggaag tcgccgtaga cgcccacgtc gcccgtggag gggtcgcggg tgaagcagac
     5281 cccggtgccc gagtcgtcgg acatgttgcc gaagaccatc gtctggatgt tgacggccgt
     5341 gccgaggtcg ttcggaatgc gctcctggcg acggtagagg acggcgcggt caccactcca
     5401 ggatcggaag acggcctcga ccgccgcgtc gagctgctgg cgcgggtcct gcgggaagcg
     5461 ctcccccgtg gcgttgaaga tcgccgtctt gaagctgcca cacagcgcgg ccaggtcggc
     5521 ggcggtcaac tcggtgtcct cgacgatccg gcgggcgtgc ttgacctgct cgatgcgcgc
     5581 ctcgaagacc tcggagtcga tccccaggac cgtcttgccg aacatctgca gcagtcggcg
     5641 gtaggagtcc caggcgaacc gctcgccggc cattccggcc agcccgagga ccgagtcgtc
     5701 gttgagcccg atgttgagaa cggtctccat catgccgggc atcgagaacg ctgcgccgga
     5761 acgcaccgac accagcaggg ggttgatcgg gtcgcccaga gtgcgtccca tcgcctgctc
     5821 caccgtggcg aggtgcgcgg tgacctcggc cgccaactcc gccggcgcct tgcccttggt
     5881 gaggtaggcc ttgcaggctt cggtcgtgat ggtgaaaccg gggggcaccg gcagcccgat
     5941 cttcaccatc tcggcaaggt tggccccctt cccgcccagg agcgccttct gttccttatc
     6001 accctcggag aagtcgaaga cgtagcgggt ggtcatgggt cgtccttccg gggaaaaggg
     6061 gcggggggtg ccgcagtgca ctcaccccat cttggaccca tttgggcctt ccgaagcggc
     6121 tctccggggg acgaaggtcc cgcagacttg acggcaccgt ggagtcgcga acgcgcttgc
     6181 gcggcaccgg gttggagccg cgacgctccc tgatcgtcaa ttccctgact agataaggac
     6241 tttcgcgtcg aggggcctcc acaacacggt atgccgcccc ccacaacgcg gtatgccgcc
     6301 cccgcaggtg cggggacggc ataccgagga ctgcgccggt gacccggcag cgactggggc
     6361 ctacaggcgc ccgtagtcgt cgaggttgag gtcgtccaga cggaccgcct cgcccgtgcc
     6421 cccaccgaag tgggagtagt cgtaggagtc gtagttgggc atggagtaca tggccgcctt
     6481 cgcctcctcg gtcggctcga cctcgatgtt gcggtagcgg gccaggcccg tgccggcggg
     6541 gatgagcttg ccgaggatga cgttctcctt gaggccgcgc agcgggtcgc tcttggcctc
     6601 catggccgcc tgggtgagca cccgggtggt ctcctggaag gacgccgccg acagccacga
     6661 ctcggtggcc aacgacgcct tggtgatgcc catgagctcg ggacgacccg aggccggctt
     6721 gccaccgtcg gtgacgaccc gacggttctc ggcctcgaac gcaccgcgct cgaccaggtc
     6781 acccgggatg aggtcggcct cgccggcctc gatgaccgtg acgcgtcgca gcatctggcg
     6841 gacgatgacc tcgatgtgct tgtcgtggat cgacacaccc tgctggcggt agacgtgctg
     6901 cacctcgtcg acgaggtgca tctgcacggc ccgcgggccg aggatacgca gcacctgctt
     6961 cgggtcgatc gcgccctgga agagctggtg accgacctcg atgtggtcgc cgtcagcgac
     7021 gagcaaccgg gcccgcttgc tcacggggta ggagtgctcc tccgcgccgt cgtccgggat
     7081 cagcacgatc cggcgagcct tgtcgctgtc ttcgatgcgg atccggccgg tcgcctcagc
     7141 gatcgacgcg acacccttgg gggtgcgggc ctcgaacaac tcgacgacac gcggcagacc
     7201 ctgggtgatg tcgtcaccgg ccacaccacc gatgtggaag gtacgcatcg tcagctgggt
     7261 gccgggctca ccgatcgact gggccgcgat gatgccgacg gcctcgccga tgtcgacgag
     7321 cttgcccgcg gccaacgaac ggccgtagca cagcgcgcag gtgccgacct tgctgtcgca
     7381 ggtgagcacc gaacggaccc ggacctcgtc gacgccggcc tcgaagagtc ggtcgatgac
     7441 gacgtcgccc aggtcgatgc cggccgtggc cagcactgtg ccgtcgacct cgacgtcctc
     7501 ggcgagcgtc cgggcgtaga ccgaggtctc gacgtcctcg tgcccgacgc gccggccctg
     7561 cgggtcgatc gtcgcgatcg gcaccttgag gccgcgttcg gtcccgcagt cctcctcgcg
     7621 gatgatgacg tcctgcgaca cgtcgaccag acgccgggtg aggtagcccg agtcggcggt
     7681 ccgcagggcg gtgtcggcca gacccttacg cgagccgtgc gtcgagatga agaactcaag
     7741 caccgacaga ccctcgcgga agttggcctt gatcggccgc gggatgatct cgcccttggg
     7801 gttggagacc aggcccttca tgccggcgat ctggcgcaac tgcatccagt tgcctcgcgc
     7861 accggagttg accatccgga agatggtgtt ggtccggggc gtgttcgcga tcatctcctt
     7921 ggcgacctcg ttggtcgcct cgttccagat ctcgatgagg tcttcgcgac gctcgttgtc
     7981 ggtcatcgca ccgcggtcgt agagcttctg caccttggcg gcgcgggctt cgtactcctc
     8041 gatgatctcg gccttgcgcg gcggggtgat gacgtcgctg atggcgaccg tcgtgcccga
     8101 gcgggtggcc cagtggaaac cggcctcctt gagggcgtcg agggacgcag cgacctggac
     8161 cttggagtag cgctcggcca gatcgttgac gatcatcgag agacgcttct tgtcgaccgg
     8221 ctcgttgacg aacgggtagt tcatcggcag cgcctcgttg aacagcgccc ggcccagggt
     8281 cgtctcgagc gtgatgaccg ggacgcggcc atcctcgccc gcctcgacgc cttcgggcag
     8341 cacgaagccg gccggcggga cggtgtcgaa caaccggatc cgcaccagcg agcccagctc
     8401 gatctggccg ctgtcgaacg ccatccgggc ctcggccggc gtgctgaacg aacgcagcgc
     8461 ctcggccccc tcaccggact ccgcggcata caccgtggtc gtctcgcagg tgaggtggta
     8521 gaggccgatg atcatgtcct gggtaggcat ggtcaccggg cgaccgtcgg ccggcttgag
     8581 gatgttgttg gacgagagca tgaggatgcg tgcctcggcc tgggcctctg ccgacagcgg
     8641 cacgtggacc gccatctggt cgccgtcgaa gtcggcgttg aacgcggtgc agaccagcgg
     8701 gtggatctgg atggccttgc cttcaaccaa ctgcggttcg aaggcctgga tgcccagacg
     8761 gtgcagcgta ggtgcccggt tgagcagcac ggggtgctcg gtgatgacct cttcgaggac
     8821 gtcccacacg accgggcgcg agcgctcgac catgcgcttg gccgacttga tgttctgcgc
     8881 gtggtcgagg tcgaccagtc gcttcatcac gaacggcttg aacagctcaa gagccatctg
     8941 cttgggcaga ccgcactggt gcagcttgag ctgcgggccg acgacgatga ccgaacggcc
     9001 ggagtagtcg acgcgcttgc cgagcaggtt ctggcggaag cggccctgct tgcccttgag
     9061 catgtcggac agcgacttga gcggccggtt gcccgggccg gtcaccggac gcccacggcg
     9121 gccgttgtcg aagaggctgt cgacggcctc ctgaagcatg cgcttctcgt tgttgacgat
     9181 gatctccggc gcaccgaggt ccaacagccg cttgagacgg ttgttgcggt taatcacgcg
     9241 gcggtagagg tcgttgaggt cggacgtggc gaaccggcca ccgtcgagct gcaccatggg
     9301 gcgcaggtcc ggcgggatga cggggacgca gtccaggacc atcgccgtgg cgctgttggt
     9361 cgtcgtctgg aaggcgttga ccaccttgag ccgcttgagg gcacgcgtct tgcgctggcc
     9421 cttgccggtg tcgatgatct cgcgcagcag gatggcctca gcgtccaggt cgaaggactc
     9481 caggcgcttc tggatcgccg cggcgcccat gccgccttcg aagtagagcc cgaaccggtc
     9541 gcgcatctcg cggtagagga tctcgtcgcc ctcaaggtcc tggaccttga ggttgcggaa
     9601 gcggtcccac acctgctcaa ggcgttcgat ctgctggtcg gcgcgacggc ggatcgagcc
     9661 catctcgcgc tcggcaccgt cgcggacctt gcgcttgatg tcggccttgg cgccctcggc
     9721 ctcgagctcg gccaggtcgg cctcaagctt cttggcacgg tcgtcgatgt cgacgtcgcg
     9781 gcggttctcc aactccttgc gctcgacctg gatctgggcc tcgagagagg gcaggtcggc
     9841 atggcgctgg tcgacgtcga ccgcggtgat catgtaggcc gcgaagtaga tgaccttctc
     9901 gaggtccttc ggggccaggt cgagcaggta gcccagacgg ctcgggacac ccttgaagta
     9961 ccagatgtgg gtgacggggg cggcgagctc gatgtggccc atgcgctcac gacgcacctt
    10021 ggcgcgagtg acctcgacgc cgcagcgctc gcagatgatg cccttgaagc ggacccgctt
    10081 gtatttccca caggagcact cccagtcccg ggtggggccg aagatgcgct cgcagaacag
    10141 cccctccttc tcgggcttga gggtgcggta gttgatggtc tcgggcttct tgacttcacc
    10201 gtgactccac tggcggatgt catccgcggt agcaagtccg atgcgcagct cgtcgaagaa
    10261 gttgacgtcc agcacgggtt tccttctctc gtccctacag tcaggtcagt tcgggggtgt
    10321 atgccgcgtg gccggcgcgg attcgcggcc tgggccacgc ggcatacctg atgtcgttag
    10381 acctcttcga cgctgctcgg ctcgcgccga ctcaggtcga tgcccagctc ctcggcggcc
    10441 cggaagacgt cgtcgtcgct ctccttcatc tcgatggcct gtccatcgct ggacagcacc
    10501 tcgacgttga gacacaggga ctgcatctcc ttgaggagca ccttgaacga ctcggggatg
    10561 ccggagtcgg ggatgttctc gcccttgacg atggcctcgt agaccttcac ccgaccagga
    10621 acgtcgtccg acttgatggt gagcagctcc tgcagcgtgt atgccgcgcc gtaggcctcc
    10681 agcgcccaca cctccatctc gccgaaccgc tgaccgccga actgggcctt accgcccagg
    10741 ggctgctgcg tgatcatcga gtaggggccg gtcgaacgag cgtggatctt gtcgtcgacc
    10801 aggtggtgga gcttgaggat gtacatgtag cccaccgaca ccggctcggg gaacggctcg
    10861 ccggaccgac cgtcgaacag ccgggtcttc ccgctgccgt cgatgagccg cacgccgtcg
    10921 cgagtcttgc gcgtcgagtc gagcagaccg gtgatgacgt cctcgcgggc gccgtcgaac
    10981 accgggctgg ccactcgggc accgggctcg gccacgtggt tgtcggccgg gatggcccgc
    11041 gcccagtcgg gcgtgccctc gatctcccac ccgcggctag ctgcccaacc caggtgcaac
    11101 tcgaggatct gcccgacgtt catgcggccg ggaacaccca tcgggttgag cacgacgtcg
    11161 accgccgtgc cgtcctcaag gaagggcatg tcctcgatcg ggaggatctt ggagatgacg
    11221 cccttgttgc cgtggcggcc ggcgagcttg tcgccgtcgg tgatcttgcg cttgttggcg
    11281 acatagaccc gcacgagctg gttgacgccg gggggcaggt cgtcgccgtc gtccttgtca
    11341 aagaccttga cgccgatgac cgtgccggtc tcaccgtggg gcaccttgag ggacgtgtcg
    11401 cgaacctcgc gggccttctc gccgaagatc gcgcgcagca ggcgctcctc cggggtcagc
    11461 tcggtctcac ccttgggcgt gaccttgccg acgagcaggt cgccgtcgcg gacctcggca
    11521 ccgatgcgga tgatcccgcg ctcgtcgagg tcggccagaa cctcttcgga gacgttgggg
    11581 atgtcccgcg tgatctcctc cgggcccagc ttggtgtcgc gggcgtcgac ctcgtgctcc
    11641 tcgatgtgga tcgaggagag gacgtcgtcc tggaccagac
//
LOCUS       DAOMZY010000063        11668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5161_length_11668_cov_5.667011, whole genome shotgun sequence.
ACCESSION   DAOMZY010000063 DAOMZY010000000
VERSION     DAOMZY010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 11668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11668
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5161_length_11668_cov_5.667011"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PLK64_05250"
     CDS             <1..415
                     /locus_tag="PLK64_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79179.1"
                     /translation="LGNHDRQGRARSAEAYRARFALPEGGTDPERVYAFTYGPARFVV
                     LDSNSASFALTDQTAWLERELAAARQDKAIRHVFVVMHHPPYSISIHGGQRDLRERWT
                     PDLLTETSARIEREGRASRGEPSDGDGARCHVRPV"
     gene            456..617
                     /locus_tag="PLK64_05255"
     CDS             456..617
                     /locus_tag="PLK64_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79180.1"
                     /translation="MTFEECAFEGANLLHASIAGSRLVDCTFAPGAWDRSIVAHVLVN
                     GAPMAGSAP"
     gene            773..2641
                     /locus_tag="PLK64_05260"
     CDS             773..2641
                     /locus_tag="PLK64_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79181.1"
                     /translation="MFKTAVWVILALLLLAAALLIWWALSRGKSQAAQRADEQQARAD
                     LAAGWGAPTTPAAAPPQFAPPPPPVAPAPPVAPPPPPPVAPAPPVAPPPPPPSWPSAA
                     AGAAATAAGAAAGSIFARPEAPQAEQAPEVAPTAEAGSMGYAAPAAPAVEAVEFETVD
                     EVEDGEFAEVVDADIEADVEVVPAPVGGIDDLWGTDPVAEAEVIEHVAGDDVAGVESG
                     DVDVDVEAAAAADADTFAEGEGGAASDADVWTPSEGPARPSPADEAAAAARARIEGDV
                     AETEAEAVEADAEAVEVVEGDAETEAAVEADAEAVEPETEVGAVEAVEADATAEADAE
                     VVEPETEVEPVEAVEVDAPEIEVAVEAAPEAEPVVEDLYDGDEAALAGSADVDAAEAA
                     YGDAESEAAALVPEVDAPEIEVAVEAAPVAEVEVDAETAPAAEVTEIDVAAEQEATAQ
                     TLEADEIALAGAPVVDDAEARFQAALDAMTAERDALADEPTDLVGPDADVPAEIVAHA
                     EAEPAEAEPAEAEAEAEAASVEEHGESTATSVGAAAAQARRRWVSEYDEVVDGGYGWG
                     SAAPVWDGAMPLGHPVKANKQWMKFQEPADGWYDKMQADVWFVDADTARRFGFTKD"
     gene            complement(2665..3879)
                     /locus_tag="PLK64_05265"
     CDS             complement(2665..3879)
                     /locus_tag="PLK64_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019159357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HPV79182.1"
                     /translation="MIGSLDAAVEGYAAGLAEQVAAAVASGRALVPSLARRLADHGLS
                     TDESLREHLSTLPILSKDDVLALQQADPPFGGALSATAAVRRVFQSPGPIYEPQLDGA
                     DPWRWSPSLQAAGMGADDLVLNCFGYHLSPAGAMFEEAALALGARVLPGGIGNQDLQA
                     RAIADLGVTAYTGLPSYLKAVIDRYDDLGLPRDRWRLQRALVTAEPLPDSLRALLRER
                     VPTVLMAYGTADTGLIAYETEPGGGLVPAPGVLVQVCDLDTGAPISQGEGQVVVTLLR
                     PDYPLVRFGTGDLSAWRLGADGSPRLVGVLGRSGAAVKVRGMFLHPRQASAVLAGVPG
                     VARWRFVIDRVEHRDDLGCEIVVAEGADDETVAADVADRIRSGLRFSCAVTSVADLPE
                     DAAPITDRRDWS"
     gene            complement(3924..4763)
                     /locus_tag="PLK64_05270"
     CDS             complement(3924..4763)
                     /locus_tag="PLK64_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV79183.1"
                     /translation="MAGSPAAPAAGAATATADLLTLNNIEVIYDDVILVLRGQSLSVP
                     QGKIVALLGSNGAGKSTTLKAISGLLPTEHGEVTDGSIIFDGQNITYLDAAARVRLGL
                     ALCMEGRHVFEHLTVEENLRAGAYFRGGGEPGDLEQVYHLLPKLADMTARVAGYLSGG
                     EQQMLAIGRALMAKPRLLMLDEPSLGLAPLLVKDIFGFIKRINDELGLTVLVIEQNAR
                     RALEVADHGYIMEQGRIVLEGSAQELRENPDVKEFYLGLGDSGNRKSFRDVKHYKRRK
                     RWL"
     gene            complement(4787..5983)
                     /locus_tag="PLK64_05275"
     CDS             complement(4787..5983)
                     /locus_tag="PLK64_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPV79184.1"
                     /translation="MKHIPRRALLAASAAVLLGLAACGGGGSAASTDTAPIKVGIIAD
                     LTGATGDVGKPYNEGMMAYIDWLNAKGGIKGRKIEAMSNDYAYQVPKAEELYKKYVSD
                     GAVVIQGWGTGDSEALRTKVSADKLPFMSASYAEVLTDPKQAAYNFVVAPTYSDQMRV
                     ALNWIAKDSGGKAEVAVFHNDSPFGTAPVADGQKWITEKSLGLGFKAYPMPKTPNFVG
                     LLSQAQSQGAKYIVIQNVSSPAAQVAKDIKAGNLDMKIVCLNWCADELFVKTAGAENA
                     EGHVMVQPFAPASANKEGSKAIEEYLKSKGSSLDAKGLHYAQGWYTMDVMAKGIEKAL
                     AGGAKLTGESIKAALESMDAVDTGGVVGTGKIKFSADSHRGSTGSGIYKIQGGKFVEI
                     AANQVP"
     gene            complement(6107..7153)
                     /locus_tag="PLK64_05280"
     CDS             complement(6107..7153)
                     /locus_tag="PLK64_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPV79185.1"
                     /translation="MATATGIHHRTYRAELRLRHTKAEWFRLALVIVLLLVVPYALNT
                     YWLGIANSILIAVVGAVGLNILVGFTGQISLGQGGFLAVGAYTSAILSTRLGLPVPLA
                     IIVAILVTAAIGALFGLPALRLKGLYLAIATLASQEIIMFVVRRWGFLTEDKGYIEVP
                     RLPFIERETFELQWYWILLPITFVAVLMARNVFRTSLGRSFMAVRDQDIAAEAIGVNI
                     TRAKVTAFALSSGFVGLSGALSAYYTETVTWERFTLDVSVLYLAMIIVGGLGSIAGSV
                     YGAIFMMLLPALLTEVGQKAGDSIPFITDQLPAIKNAIFGIAIITFLLIEPRGLDRIW
                     TRMKDYVRFWPFRY"
     gene            complement(7157..8029)
                     /locus_tag="PLK64_05285"
     CDS             complement(7157..8029)
                     /locus_tag="PLK64_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPV79186.1"
                     /translation="MSNFLLLTVYGLADGAILALAALGFVLIYKATRVINFAQGEFLL
                     FGAYMFYTAFVVMRLPWILASVVGVIGAIILGVLVERFVLRPLVGESAISVIMVTIGL
                     SSVLKALVQMFFGTSVREQPKILPTGSIEFLGATMPVNRLLVILIAAVVLAAFTIFFR
                     RSRHGIAMRAVADDQQAALTMGISVRQIFALAWALAAVSALVAGVLLADLSAVEQNIA
                     SFGLIVFPVVILGGLDSIPGTIVGGLTIGLLKQYVSGYADPGLAEVIPYVVLVAILMV
                     RPYGIFGETRIERV"
     gene            complement(8026..10035)
                     /locus_tag="PLK64_05290"
     CDS             complement(8026..10035)
                     /locus_tag="PLK64_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPV79187.1"
                     /translation="MSASDATFPKLLRGLATKNPDQTALQEKRYGIWQPMSWSTYAAR
                     VQDFAHGLAALGVERGQIVAVLGDNRPEWLIAELAAQSIGAAVVGIYPTSIGEELLHI
                     LTLAQVKVVVAEDQEQVDKLLKLRAEAAAAAVDPSAATSAPFADMPPLLVDTVVFYDP
                     HGLEQYTDECLRDFTAVEALGREWGATHPGWLDEQVARGTSSDIAVICTTSGTTSRPK
                     LAELSHDNLLAMADHLTAIDPIDSNDRYVSFLPFAWIGEQMLAVACGLSRGLTLSFPE
                     DSATQKSDMREIGPDVMFSPPRIWEGMLSEVQVRIDEAGWLKRKVFGWGYDVGDKAAG
                     RRVKGQGAGALGALHWLADQVALRPVRDQLGLARNQHAYTGGAPLGPDVFRFFHAIGV
                     NLKQLYGQTEICGIAVVHRDDDIAFNTVGTPIAGTEIQIADNGEILLRSASVFKGYHR
                     NPAATAEAVDPEGWLHTGDAGYLDDNGHLVVIDRQKDVLTTADGTRFSSAFIENKLKF
                     SPYVEEAVTFAADNGMTAIITIDPMTTGSWAEHERLSYTTYTDLAAKAEVQDLVAEEI
                     HRANEDLPESIRVQRFVLLHKQFDPDDDEITRTRKVRRNVIQDRYGLIIDALNRGDER
                     IGITTTVTYQDGTKVDRTIDLRIFDLTTYRVPEGRGRRPVWSGRR"
     gene            complement(10035..10874)
                     /locus_tag="PLK64_05295"
     CDS             complement(10035..10874)
                     /locus_tag="PLK64_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009884953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV79188.1"
                     /translation="MLGRAYVHSLNSSTVPVGSPILEISDIELRFGGVVALHDVGFTV
                     TQGHIHAVIGPNGAGKSSLLNCVSGLYHPQKGKAVLHTGDGAVTHELTKLPPHRIARL
                     GVARSFQNIELFSHLTVLENLMLGRHTHMKHGVLQSLLWFGPARNQELSHRGIVEEVI
                     DLLSLQPFRHKPVGSLAYGIQKRVELGRALVLQPSLLLLDEPMAGMNSEEKEDMARYV
                     LDVHELAGVTVILIEHDMAVVMDISDRVSVLDFGRLIADGTPDEVKANSAVIEAYLGA
                     EEH"
     gene            11037..11660
                     /locus_tag="PLK64_05300"
     CDS             11037..11660
                     /locus_tag="PLK64_05300"
                     /inference="COORDINATES: protein motif:HMM:NF020897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV79189.1"
                     /translation="MPPLSDALQEAPGSLGTVRNAALLLQLLAEGPAFQQLTDLADRS
                     GLSLATVHRLLRSLVLADLVEQDPQSSRYGLGPELTRLSHRRLARLPVLGALAPYLGQ
                     VRDAVGGTVRVDVVVRTAVVSIDRIDGSGAGVGLYRDPHGPRLALDTVAGRLLLSRAD
                     DERWKQALAEVDEPTRALAEHERDDWAKAAWLRADGPFPGAPPEVGA"
BASE COUNT         1849 a         3783 c         4217 g         1819 t
ORIGIN      
        1 gctcggcaac cacgatcgcc agggccgcgc gcgctcggcc gaggcctacc gcgcgcggtt
       61 cgcgctgccc gagggcggca ccgatcccga gcgggtctac gcgttcacct acgggccggc
      121 gcggttcgtc gtcctcgact ccaactcggc gtcgttcgcg ctgaccgatc agaccgcgtg
      181 gctcgagcgc gagctggcgg cggcgcgcca ggacaaggcg atccgccacg tcttcgtcgt
      241 gatgcaccac ccgccgtact cgatctcgat ccacggcggc cagcgcgatc tgcgcgagcg
      301 gtggacgccg gatctgctca ccgagaccag cgcgcgcatc gagcgggagg gtcgagcgtc
      361 ccggggcgaa ccttcggacg gcgatggcgc acggtgccac gttcgtccgg tgtgacttct
      421 ccggagccga cctgaggatc acccacctcc accgggtgac gttcgaggag tgcgccttcg
      481 agggagccaa cctcctccac gcgtcgatcg ccgggtcgcg gctggtcgac tgcacgttcg
      541 cgcccggcgc gtgggatcgc tccatcgtcg ctcacgtcct ggtcaacggt gctcccatgg
      601 cggggagtgc gccgtagtca gttggggttg gtttacctgc cccgtaccta cgccgcgaac
      661 gtgggcatcg ccctcggccc acgtccggtg aacattctga agacgagacc ggtatgttgc
      721 cgtctgggga tgcgtcaggg cgcgtcctct cctgacacct gaaggtggaa ccatgttcaa
      781 gaccgccgtg tgggtcattc tggccctcct cttgctggca gccgcgctgc tcatctggtg
      841 ggcgctgagc cgcgggaaga gtcaagccgc tcagcgcgcc gacgagcagc aggctcgggc
      901 cgacctggcc gccggatggg gcgccccgac gactcccgct gccgctcccc cccagttcgc
      961 gcccccaccg ccgcccgtcg cgcccgctcc tcccgtggcg cccccgcccc cgccgcccgt
     1021 cgcgcccgct cctcccgtgg cgcccccgcc gccgccgccg tcatggccga gcgcagcggc
     1081 aggtgccgcc gcgacggccg ctggtgcggc cgcgggttcg atcttcgcgc gccccgaggc
     1141 accgcaggct gagcaggcgc ccgaggtcgc gccgaccgct gaggcggggt cgatggggta
     1201 tgccgcgccg gcggctcccg cggtcgaggc cgttgagttc gagactgttg acgaggtcga
     1261 ggacggcgag ttcgcagagg tcgttgacgc tgacatcgag gctgacgttg aggtcgttcc
     1321 ggcccccgtg ggtggcatcg acgacctgtg ggggaccgac ccggtggccg aggctgaggt
     1381 catcgagcac gtggctggcg acgatgtcgc cggggtcgag tctggcgacg tcgacgtcga
     1441 cgtcgaagct gctgctgctg ccgacgctga caccttcgct gagggtgagg gtggcgccgc
     1501 ctcggatgca gacgtctgga ctccgtcgga aggtcctgct cgcccgtcgc ctgccgacga
     1561 agccgcggcc gctgcccggg cccggatcga aggtgacgtt gccgagaccg aggctgaggc
     1621 tgtcgaggcg gacgccgagg cggttgaggt tgtcgagggc gacgctgaga ctgaagccgc
     1681 tgtcgaggcc gacgctgagg cagtcgaacc cgagactgag gtcggggcgg ttgaggcggt
     1741 cgaggccgac gccactgccg aggccgacgc ggaggtcgtt gaacccgaga ctgaggtcga
     1801 gccggttgag gccgtcgagg tcgatgcccc ggagatcgag gtggccgtcg aagctgcccc
     1861 tgaagccgag cctgtggtcg aggacctgta tgacggcgac gaggctgcct tggccgggtc
     1921 ggcggacgtc gacgccgcag aggcggccta tggtgacgcg gagtcagaag cagccgcgct
     1981 ggtgcccgag gtcgatgccc cggagatcga ggtggccgtc gaagctgcac cggtcgccga
     2041 ggtcgaggtg gatgccgaaa cggctccagc cgcggaggtc acggagatcg atgtggctgc
     2101 cgagcaggag gccacggcgc agacccttga agccgacgag atcgccttgg ccggcgcacc
     2161 ggtggtcgac gatgcggagg cgcgcttcca ggcggccctg gacgcgatga cggccgagcg
     2221 ggacgccctg gccgacgagc cgacggacct ggttggtccc gacgccgacg tgccagcaga
     2281 gatcgtggca cacgccgagg ccgagcctgc cgaagccgag cctgccgaag ccgaagccga
     2341 agccgaagcc gcgtcggtcg aggagcacgg tgagtcgaca gccacctcgg tgggcgccgc
     2401 agccgcgcag gcccgccgcc gctgggtgtc cgaatacgac gaggtcgtcg acggcggcta
     2461 tggctggggg tcagcagcgc ccgtatggga cggcgccatg cccctaggtc acccggtcaa
     2521 ggccaacaag cagtggatga agttccagga gcccgccgac ggctggtacg acaagatgca
     2581 ggccgacgtg tggttcgtcg atgcagacac cgctcgccgg ttcggcttca cgaaggactg
     2641 atctggggct gaggccgcag gcccctagga ccagtcacgc cggtcggtga tcggcgcggc
     2701 atcctcgggg aggtcggcca ctgacgtgac cgcgcacgag aaccgcaacc cggagcggat
     2761 ccggtcggcg acatcggcgg cgaccgtctc gtcgtcggca ccctcggcga cgacgatctc
     2821 gcaccccagg tcgtcacggt gctcgacccg atcgatgacg aagcgccaac gggccacccc
     2881 gggcactccg gcaagcacgg cggaggcctg ccgtgggtgc aggaacatcc cgcgcacctt
     2941 gacggctgct ccggagcggc cgaggacccc gacgaggcgg ggcgacccgt ccgcgccgag
     3001 ccgccaggcc gacaggtcgc cggtgccgaa ccgcaccagc gggtagtcgg ggcggagcaa
     3061 cgtgacaacg acctggcctt caccctgcga gatcggcgct ccggtgtcga ggtcgcacac
     3121 ctgcacgagc acgcccggag caggcaccaa cccacccccc ggctcggtct cgtaagcgat
     3181 cagtccggtg tcggcggttc cgtaggccat gaggacggtc ggcacccgct cgcgcagcag
     3241 ggcgcgcagc gagtcgggga ggggctcggc cgtgaccagc gcgcgctgca ggcgccaccg
     3301 gtcgcggggc aggcccaagt cgtcgtagcg gtcgatgacc gctttgagat agctgggcag
     3361 gccggtgtat gccgtcaccc ccaggtctgc gatcgcccgc gcctgcaggt cctggttgcc
     3421 gattcctccc ggcagcactc gggccccgag ggcgagggca gcctcctcga acatcgcacc
     3481 ggcgggggac aggtgatagc cgaagcagtt gagcaccagg tcgtcggcgc ccatcccggc
     3541 ggcctgcagc gaggggctcc accgccaggg atcggcaccg tcgagctggg gttcgtagat
     3601 cgggccgggg gactggaaga cccgccggac cgcagcggtg gccgacagag cgccgccgaa
     3661 cggcgggtcg gcctgctgga gggccaacac gtcgtccttg gacaggatcg gcaacgtcga
     3721 gaggtgctcg cgcaggctct cgtcggtgga caacccatga tcggccaggc ggcgggcgag
     3781 cgagggcacc aacgcccgcc ccgacgcgac ggcggcagca acctgctcag ccagccccgc
     3841 ggcatacccc tcgacggcag cgtccagcga cccgatcatg gcgtgcatcc ctttcccgga
     3901 agcggtggtg acctgcgatg cggtcagagc cagcgcttgc ggcgcttgta gtgcttcaca
     3961 tcgcggaagc tcttgcgatt gccggagtcg ccgaggccga ggtagaactc cttgacgtcg
     4021 gggttctcgc gcaactcctg agccgagccc tccaacacga tccggccctg ctccatgatg
     4081 tagccgtggt cggcgacctc cagggcgcgc cgggcgttct gctcgatgac cagaacggtc
     4141 aaccccaact cgtcattgat ccgcttgatg aagccgaaga tgtccttgac gagcaacggg
     4201 gccaggccga gcgacggctc atcgagcatg agcagacgcg gcttggccat gagcgcacgg
     4261 ccgatggcga gcatctgctg ctcaccgccg gacaggtagc cagccacccg ggcggtcatg
     4321 tcggcgagtt tggggagcag gtgatagacc tgctcgagat cgccgggctc gccgccaccg
     4381 cggaagtagg cccctgcgcg caggttttcc tcgaccgtga ggtgctcgaa gacgtgccga
     4441 ccctccatgc acaacgcgag cccgagcctc acccgggctg cggcgtcgag gtaggtgatg
     4501 ttctgcccgt cgaagatgat cgagccatcg gtgacttcgc cgtgctcagt cggcagcagc
     4561 ccggagatcg ccttgagagt cgtcgacttc ccggcgccgt tcgagccgag caacgcgacg
     4621 atcttgccct gcgggaccga cagtgactgt ccgcgcagca cgaggatgac gtcgtcgtag
     4681 atgacctcga tgttgttgag cgtcagcagg tcagccgttg ctgtcgcggc ccccgctgcg
     4741 ggggcggccg gcgaaccggc cacccccgac gcgtcctggg acgtgctcac gggacctggt
     4801 tcgccgcgat ctcgacgaac ttgccgccct ggatcttgta gatgccagat ccggtggagc
     4861 cacggtggga gtcggcgctg aacttgatct tgccggtgcc gacgacgccg ccggtgtcca
     4921 ccgcgtccat cgactcgagc gcggccttga tcgactcacc ggtgagcttg gcgccgccag
     4981 cgagggcctt ctcgatgccc ttggccatga cgtccatcgt gtaccagccc tgggcgtagt
     5041 gcaggccctt ggcgtcgagc gaggacccct tgctcttgag gtactcctcg atggccttgc
     5101 tgccttcctt gttcgccgag gcgggtgcga acggctggac catgacgtgg ccctcggcgt
     5161 tctcggcgcc ggcggtcttg acgaacaact cgtcggcgca ccagttgagg cagacgatct
     5221 tcatgtcgag gttgccggcc ttgatgtcct tggcgacctg ggcggccggg ctggacacgt
     5281 tctggatgac gatgtacttc gcgccctgcg actgggcctg cgacagcagc ccgacgaagt
     5341 taggcgtctt gggcatcggg taggccttga agcccagccc caggctcttc tcggtgatcc
     5401 acttctggcc gtcggcgacc ggggcggtgc cgaacgggct gtcgttgtgg aagacggcga
     5461 cctcggcctt gccgccggag tccttggcga tccagttgag cgcgacgcgc atctggtcgg
     5521 agtaggtcgg ggcgacgacg aagttgtatg ccgcctgctt ggggtcggtc agcacctcgg
     5581 cgtaggaggc cgacatgaag ggcaacttgt cggcagagac cttggtgcgc agcgcctcag
     5641 agtcgccggt gccccagccc tggatgacca cggcgccgtc cgagacgtac ttcttgtaga
     5701 gctcctcggc cttgggcacc tggtaggcgt agtcgttgga catcgcctcg atcttgcggc
     5761 ccttgatgcc gcccttggcg ttgagccagt cgatgtaggc catcatgccc tcgttgtagg
     5821 gcttgccgac gtcgccggtc gctcccgtca ggtcggcgat gatgccgacc ttgatcggcg
     5881 cggtgtccgt ggaggccgcg ctgccgcccc cgccgcaggc ggcgaggcca aggagcacgg
     5941 ctgcagaggc ggcgagcaag gcccgcctgg gaatgtgctt cataggtgtg actccatctt
     6001 ctgcggggtg ccggggaacc ggcgaggggg tgcgtcggtt gcggactggg gagggtgcgg
     6061 aggtgcgggc acccggtatg ccgcgggcgc ggcataccgg atcgggctag tagcggaagg
     6121 gccagaaccg gacgtagtcc ttcatccgtg tccagatgcg atcgagccct cgaggctcga
     6181 tgaggaggaa cgtgatgatg gcgatgccga agatggcgtt cttgatggcg ggcagttggt
     6241 cggtgatgaa ggggatcgag tcgccggcct tctggcccac ctcggtgagg agcgcgggca
     6301 ggagcatcat gaagatcgcg ccgtagaccg acccggcgat gctgcccagg cctccgacga
     6361 tgatcatcgc gaggtagagg accgagacgt cgagggtgaa ccgctcccag gtgactgtct
     6421 cggtgtagta ggcggacagt gcgccggaca ggccgacgaa tccgctggac agggcgaagg
     6481 cggtcacctt ggcccgggtg atgttgacgc cgatggcctc ggcggcgatg tcctggtcgc
     6541 ggacggccat gaacgagcga ccgagtgacg tgcggaagac gttgcgggcc atgaggacgg
     6601 ccacgaacgt gatcggcagc aggatccaat accactgcag ttcaaaggtt tcccgctcga
     6661 tgaacggcag gcggggcacc tcgatgtaac ccttgtcctc ggtgaggaag ccccaccgcc
     6721 ggacgacgaa catgatgatc tcctggctgg ccagcgtggc gatcgccaga tagagaccct
     6781 tgagccgcag cgcgggcagc ccgaacagcg cgccgatggc ggccgtgacg aggatggcca
     6841 cgatgatcgc cagcggcacc ggcaggccga gccgggtgga caggatggcc gaggtgtagg
     6901 cgccgacggc gaggaagccg ccctggccga gggagatctg gccggtgaag cccacgagga
     6961 tgttgaggcc caccgcgccg acgacggcga tgaggatcga gttggcgatc cccaaccagt
     7021 aggtgttgag ggcgtagggg acgacgagga gcagcacgat caccagggcg aggcggaacc
     7081 attcggcctt ggtatgccgc aggcgcagct ccgcgcgata ggtgcggtgg tggattccgg
     7141 ttgcggtagc catgggtcag accctctcga tccgggtctc gccgaagatg ccgtacgggc
     7201 gcaccatgag gatggcgacg aggacgacat aggggatgac ctccgctaga ccgggatcgg
     7261 cgtagccgga gacgtactgc ttgagcaggc cgatcgtcag accgccgacg atggtgccgg
     7321 ggatcgagtc gaggccgccg aggatgacga ctgggaagac gatgaggccg aacgacgcga
     7381 tgttctgctc gacggccgag aggtcggcca gcaggacacc ggccaccagc gcgctcacgg
     7441 cggccaaggc ccacgcgagg gcgaagatct gccggaccga gatgcccatg gtcagggccg
     7501 cctgttggtc gtcggcgacg gctctcatgg cgatgccatg gcgggaccga cggaagaaga
     7561 tggtgaaggc cgccaggacg accgcggcga tgaggatgac gagcagtcgg ttgaccggca
     7621 tcgtcgcgcc gaggaactcg atgctgccgg tggggaggat cttcggctgc tcgcgcaccg
     7681 aggtgccgaa gaacatctgc accaaggcct tgagcaccga cgagagcccg atcgtcacca
     7741 tgatgacgct gatcgcgctc tcgccgacca atgggcgcag gacgaagcgc tcgacgagga
     7801 caccgaggat gatcgccccg atgaccccca cgacgctggc gaggatccac ggcagacgca
     7861 tgacgacgaa cgccgtgtag aacatgtagg cgccgaagag aaggaactcg ccttgggcga
     7921 agttgatgac tcgggttgcc ttgtagatga ggacgaagcc cagtgcggcc agcgcgagga
     7981 tcgcgccgtc ggccaagccg tagacggtga ggaggaggaa gttgctcatc ggcggccact
     8041 ccacacgggg cgtcgtccgc ggccctcggg cacgcggtag gtggtcaggt cgaagatgcg
     8101 caggtcgatc gttcggtcga ccttggtgcc gtcctggtag gtgacggtcg tcgtgatgcc
     8161 gatgcgctcg tcaccgcggt tgagggcgtc gatgatgagg ccgtagcggt cctggatgac
     8221 gttgcgccgg accttgcggg tccgggtgat ctcgtcgtca tccgggtcga actgcttgtg
     8281 cagcaacacg aaccgctgca cccggatgct ctcgggcagg tcctcgttgg cgcggtggat
     8341 ttcctcggcg accaggtcct ggacctcggc cttggcggcc aggtcggtgt aggtcgtgta
     8401 ggacagccgc tcgtgctcgg cccaagaacc ggtcgtcatc gggtcgatgg tgatgatcgc
     8461 cgtcatgccg ttgtcggcgg cgaaggtgac cgcttcttcg acgtaggggg agaacttcag
     8521 cttgttctcg atgaaggccg aggagaaccg ggtgccgtcg gccgtcgtga ggacgtcctt
     8581 ctgccggtcg atgacgacaa ggtggccgtt gtcatcgaga tagccggcgt cgccggtgtg
     8641 caaccacccc tcggggtcga cggcctcggc cgtggccgcc gggttgcggt ggtagccctt
     8701 gaacaccgac gccgagcgca gcaggatctc gccgttgtcg gcgatctgga tctcggtgcc
     8761 cgcgatgggg gtgccgacgg tgttgaaggc gatgtcgtcg tcgcggtgaa cgacggcgat
     8821 gccgcagatc tcggtctggc cgtagagctg cttgaggttg acgccgatgg cgtggaagaa
     8881 ccggaagacg tcgggcccga gtggagcgcc gccggtgtag gcgtgctggt tgcgggccag
     8941 cccgagttgg tcgcggacgg ggcgcagcgc gacctggtcg gccagccagt gcagcgcgcc
     9001 gagcgcgccc gcaccctggc ccttgacccg ccggccggcg gccttgtcgc cgacgtcgta
     9061 gccccagccg aagaccttgc gcttgagcca gccggcctcg tcgatgcgga cctggacctc
     9121 cgagagcatc ccctcccaga tccggggcgg ggagaacatg acgtccgggc cgatctcgcg
     9181 catgtccgac ttctgcgtcg ccgagtcctc cgggaaggac agggtcaggc cgcgggagag
     9241 tccgcaggcg acggcgagca tctgctcgcc gatccacgcg aacggcagga acgacacgta
     9301 gcggtcgttg ctgtcgatcg ggtcgatcgc ggtgaggtga tcggccatgg ccaggaggtt
     9361 gtcgtgcgac aactcagcca gcttgggacg gctcgtcgtt ccggaggtcg tgcagatgac
     9421 ggcgatgtcc gaggacgtgc cgcgggcgac ctgctcgtcg agccagccgg ggtgcgtggc
     9481 gccccactcg cggcccagtg cctcaacagc ggtgaagtcg cgcaggcact cgtcggtgta
     9541 ttgctccagg ccgtgcgggt cgtagaagac gacggtgtcg accagcagcg gcggcatgtc
     9601 ggcgaacggt gccgaggtgg ctgcgctcgg gtcgaccgcc gcggcggcag cctccgcgcg
     9661 cagcttgagc aacttgtcga cctgctcctg gtcttcggcg acgacgacct tgacctgggc
     9721 cagggtgagg atgtgcagca actcctcacc gatgctcgtc gggtagatgc cgacgacggc
     9781 ggcgccgatg ctctgcgcgg ccaactcggc gatgagccat tcggggcggt tgtcgccgag
     9841 gaccgcgacg atctggccgc gttcgacccc gagcgcggcc aggccgtgcg cgaagtcctg
     9901 gacgcgcgcg gcatacgtgc tccagctcat cggctgccag atgccgtagc gcttctcctg
     9961 caaggcggtc tggtcggggt tcttggtcgc caacccgcgc aacaacttcg ggaaggtcgc
    10021 atcactcgcg ctcatcagtg ctcctccgcc ccgagatagg cctcgatgac cgcggagttg
    10081 gccttgacct cgtcgggagt gccgtcggcg atgagccggc cgaagtcgag caccgagacg
    10141 cggtcggaga tgtccatgac gacggccatg tcgtgctcga tgaggatgac cgtgacgccg
    10201 gcgagctcgt ggacgtcgag gacatagcga gccatgtcct ccttctcctc ggagttcatg
    10261 ccggccatcg gttcgtcgag cagcagcagc gacggttgca gcaccagcgc ccggcccagc
    10321 tcgacccgct tctggatgcc gtaggcgagc gaccccaccg gcttgtggcg gaagggctgc
    10381 agcgagagca ggtcgatgac ctcctcgacg attccgcggt gagaaagctc ctggttgcga
    10441 gctggcccaa accacagcag agactgcagc acgccgtgtt tcatgtgggt atgccgcccc
    10501 aacatgaggt tctccagcac cgtgaggtga gagaacagct cgatgttctg gaacgagcgg
    10561 gccacgccca gccgggcaat cctgtgcggc ggaagcttgg tcagctcgtg ggtgaccgca
    10621 ccgtcaccgg tgtgcaggac cgccttgccc ttctgcgggt ggtagaggcc actgacgcag
    10681 ttgagcaggc tggacttgcc ggcaccgttg gggccgatca ccgcatggat gtgaccctgc
    10741 gtgacggtga accccacatc gtgcagggcc acgaccccgc cgaaccgaag ttcgatgtcg
    10801 gagatctcga ggatcgggct ccccacgggg accgtggagg agttgagcga gtgcacatag
    10861 gctcggccca gcaccgcgac ctccttgtcg acgacgacgg gtggttcaag cggactctac
    10921 ttttccacta tctggaaacg caatgcttct tgtggaaatc gttacctcgg cctacagtcg
    10981 agcaccgagc cgggcctgac gtgcgggacg ctcggcacgg aaggagtttg gtaccggtgc
    11041 cgcctctcag cgacgcactg caagaagcac ctgggtcatt gggaacagtt cgcaacgccg
    11101 cgctcctgct gcagttgctg gccgaagggc cggccttcca acagctcacg gacctcgccg
    11161 atcgctccgg actctcgctg gcgacggtcc accgactgct gcgcagcctc gtcctcgccg
    11221 acctggtcga gcaggatccg cagtcctcgc gctatggcct aggccccgag ttgacccgcc
    11281 tgtcccaccg ccgcctggcc cgcctgccgg ttctcggcgc cctcgcgccc tacctcggtc
    11341 aggtccgcga cgccgtgggc ggcaccgtcc gggtcgatgt cgtcgtgcgg accgcagtgg
    11401 tgtcgatcga ccggatcgac ggctcggggg ccggggtcgg gctctaccgg gacccgcacg
    11461 gcccgcgcct ggccctggac acggtcgccg ggaggctgtt gctctcccga gccgacgacg
    11521 agcggtggaa gcaggccctg gccgaggtcg acgagcccac tcgcgcgctc gccgaacacg
    11581 agcgcgacga ctgggcgaag gcggcctggc tacgcgccga cgggccgttc cccggcgcgc
    11641 ctcccgaggt cggcgcgtag ccaggccg
//
LOCUS       DAOMZY010000064        11632 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5179_length_11632_cov_6.369785, whole genome shotgun sequence.
ACCESSION   DAOMZY010000064 DAOMZY010000000
VERSION     DAOMZY010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 11632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11632
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5179_length_11632_cov_6.369785"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(256..522)
                     /locus_tag="PLK64_05305"
     CDS             complement(256..522)
                     /locus_tag="PLK64_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79190.1"
                     /translation="MAWQPVKADPTSAQVVVQAVVGGIPCDRVTGIEAVETGSTVAIS
                     VWAGPEVGATGCDGPQPALAQIVWVRVPLAGVLGSRTVLPYSPS"
     gene            complement(891..2018)
                     /locus_tag="PLK64_05310"
     CDS             complement(891..2018)
                     /locus_tag="PLK64_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79191.1"
                     /translation="MKKSTLPTRLLALGAVFATAAALAVGSAGSAQAAKPGARGDVSS
                     ATASISAEAKAAGVAKHGAKATDAQALEAYWTPERMRAAKPAEEDSTLEASATAYVQK
                     QVADQKAGRKPVTNDGPVRSVEGASSKVFGPAATAGGVAAAYNPNLPYYVPTAYTAGK
                     VFFSKPDGNYVCSGTIINSEGKDTVWTAGHCVHGGSGGTWHTNWTFVPAYDDDLANPR
                     PYGTWSANQLWSRTAWTNSSDFSQDMGVAIMNTNWGWHIVDYFGGQGLTTNRGKSGVW
                     ENAFGYPAEWPFDGGNLMQCWGSTSPEWDAWFFWSQTVKIPCDMTRGSSGGGWLYGYN
                     GDWGQLNGVNSRIDRISGPTIMLSPYFDDDAWSLFNSTRWL"
     gene            complement(2152..2724)
                     /locus_tag="PLK64_05315"
     CDS             complement(2152..2724)
                     /locus_tag="PLK64_05315"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPV79192.1"
                     /translation="MSAGDVPVTGGPASAPADDAAYEDSEQGYAAGLRAWDGLPGIPA
                     SSGALIRDSRGRILVLKPTYKSGWTIPGGVMEANGETPWEACQREVFEETGLRVSAGR
                     LAAVDTRPAKARRAMGLRFLFDCGVVTDEQAASITLQSTELSDHAFLAPSEALARLRP
                     AVSRRVAAVLETGGCRYLEDGRPVAGVPDE"
     gene            2789..3316
                     /locus_tag="PLK64_05320"
     CDS             2789..3316
                     /locus_tag="PLK64_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HPV79193.1"
                     /translation="MTTPRFAAALNAQIGNEFAAHLQYVAIAVHFDALTMPQMASFFY
                     VHAREERDHAMMMVRYLLDTEAEVTIPGSEAPITRFDGVVEPIELSLSQERTVTEQVY
                     ALTRIAREENDYGAEQFMQWFIKEQVEEVAKMSSLLTVAKRNTDSIADLEAYVAGQQA
                     NGGGVDPTAPSIADA"
     gene            complement(3402..4055)
                     /locus_tag="PLK64_05325"
     CDS             complement(3402..4055)
                     /locus_tag="PLK64_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPV79194.1"
                     /translation="MTVRVVVIDDQTLVRQGIVGLLRLSGEVEVIGQASDGAEGLEVV
                     AALRPDVVLLDLRMPILDGLGVLQALAAAGDRTPVLVLTTFPDDEALIAAMRAGARGY
                     LLKDVSLDQLVDAITTLAGGGTIIQPSVTEALVRQLAGTSADVDPVGLAPEPLTARET
                     EVLRLLSGGYSNREIAAAMFLAEGTIKNHVSAVLLKLGVRDRTRAVLRGLELGLIQR"
     gene            complement(4052..5233)
                     /locus_tag="PLK64_05330"
     CDS             complement(4052..5233)
                     /locus_tag="PLK64_05330"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPV79195.1"
                     /translation="MQERHLEPLGIATAGMAWLSIGVVELIGLSDRASNWWWLAYLGY
                     GAAYLWCVVVAPKGHWPRRERAIALGVVTPLGVAVVALSSSSGFTSISLVMTAILLAN
                     LVSVRVVVGIVLVQTLAVVALLWTTFSDGGMAYAVSTGVAFFAFQLFALLMVVSVRAT
                     EAARNEADRARGEAEAANAALRAAQTQLATTSRTQERLRIARDLHDVVGHQLTALSVN
                     LQVASRTATGPAKDDLEVCRAVASDLLRDVREVVSQLRETPSAGPEFAEELRTIGHSV
                     PSPVVDVDVADDLPPLGAAEADVVRRCIQEIVTNAVRHSGATSVWIAVEELDGVVSVT
                     ARDDGQGSGGDGADVVMGHGLTGMVERFEALGGSVAWTARPGQGFTLQASVPVGRVAA
                     T"
     gene            5296..6348
                     /locus_tag="PLK64_05335"
     CDS             5296..6348
                     /locus_tag="PLK64_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV79196.1"
                     /translation="MTTGTDTGVCRPTTVDDMTSTPASGSTTTPAVIRASGLTKRYGD
                     LVAVDALDLVIAPGDIVAILGPNGAGKSTTIEMLLGLRTPTAGEVRVFGDAPNAKATR
                     ARVGAMLQESSAPESLTVAEAVDLVRHYYPFALSLDDILDRADLSEKRGNRIAQLSGG
                     QQQRLNFALAIAGDPDLLFLDEPTAALDVEARHLFWEQVRGLAAHGKTILFSTHNLAE
                     ADALADRVILINRGRVIADGTPEQIKATVATRTVRLVTDVGVAALAAFPGVRGVEVAP
                     GESVGATHPTLVVQTATPEDLLARLFAGGHLVSQLTVTDTDLETAFIHLVGATTQTAP
                     TATPDTTPEHVMESAS"
     gene            6345..7235
                     /locus_tag="PLK64_05340"
     CDS             6345..7235
                     /locus_tag="PLK64_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV79197.1"
                     /translation="MSTAGRTAYPADSSTTDSGDRTPRRLSPEASRMPGALGTLAAQL
                     RAELLSSSRVPEYVVGVVAVPIILFTMFGLPNAGVKLPGGTDVGAMMFASMSAYGVVS
                     LAIFTFGVGVADERGKGWLRRMRATPMPFWAYFVAKVVGSLAFCGLILLGTWAVAALG
                     GKVPFDGVRLAQTVLILLAGTVAFSTMGFSLAYWFRPKAASAIGNLVFLPLSFLSGFF
                     FPLDQLPDVLAKVAPYLPTYHFGQLVWSAIAPAQDVIAFGAHPEGSAWVHVGWVLASF
                     VGFGLLARAGYRRELSRAAQ"
     gene            7313..7873
                     /locus_tag="PLK64_05345"
     CDS             7313..7873
                     /locus_tag="PLK64_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPV79198.1"
                     /translation="MTETPAAAAPVGDDLSLSHALLIDAFGRVAEGVPALLDGLGGEE
                     ILWRPDEDSNSIGWLVWHLARVQDDHLAGVAGREQIWTTAGFAKRFDLPYAARSIGYG
                     QSSQEVAAFSVADGSLLAAYHAAVHAETVEIVQGLTAADFARIVDRRWDPPVTAAVRL
                     VSVVNDITQHAGQAAYLRGLVERRRA"
     gene            7954..9249
                     /locus_tag="PLK64_05350"
     CDS             7954..9249
                     /locus_tag="PLK64_05350"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HPV79199.1"
                     /translation="MLTAFAVLLCLSALLAWANERWVHLPSTVAVTLAGAASSIVLIL
                     LDTQGWTFGLKQQAIELWQALDFTAFVLNGILSLMLFAGAMSLDATLMMRLRAGILTF
                     AGVSTAISTVLIGFGAWGVFHLVGLDIPLIWSLLFGALLSPTDPVAVLDMLKRAKVPK
                     KIETLIAGESLFNDGVGVVFFIVIASAAGVGGGHTEPSLTGALVVFVQEALGGIAFGA
                     LIGWITYRLCRSIEAASVEILLTLASVVGGYVLAASLGLSGPLAMVVAGLVMSAGKER
                     AFGEHTRERIEGFWETLDEVLNILLFAFIGLDVLLTPTAVGEVLAGLLMIVVALVARF
                     VSVALPMTLVRRRDGYGPWTIRVLTWGGLRGGIAISLALGLPASPYRSALVTATYVVV
                     LFTIAVQGLTVMPIVRRAAAAEAAAEAAAGETENRAARP"
     gene            9357..9878
                     /locus_tag="PLK64_05355"
     CDS             9357..9878
                     /locus_tag="PLK64_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79200.1"
                     /translation="MLTVFLAIGAVGLALLVISLVVGEIHGGLFEWIDNDLFSGAAVS
                     AFLSALGFVGALVESTSGNTTVATLAGTGAGVGMAYAARGLTRSLQSGEGDTTVRSGD
                     LVGHRGHVVEAIPRDGMGMVSVSVAGHLTRLNARCEQPLSAGTGIEVTSVLSPTAVTV
                     RPIVVTPQDRALD"
     gene            9909..11387
                     /locus_tag="PLK64_05360"
     CDS             9909..11387
                     /locus_tag="PLK64_05360"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPV79201.1"
                     /translation="MSANEFPVILVAIVGLIILLVVVGVLVASRYKVAGPNEAFIVTG
                     RKGKEIRDVETGEVSTDLSGQKVVMGGGIFVVPFVQRLHVLDLSSRRIMIQIRGAVSG
                     QGVKLNLDGVALVKVGGNEDSIRAAAQRFLTQQREIETFTQETLAGSLRSIVGSLTVE
                     QIIRDRAAFAQRVADESETSLTGQGLVLDTFQIQDITDDGSYLSDLGRPEAARVGQTA
                     AIAEANARQAAEQARLAAEQEIAIANRELALKQAEIKAQTDAATAQAAAAGPLAQADR
                     DQAILAEQEKVAVRKAALTERQLDTEVRRPADAARYRVEQEADARKAASIAAAQADAE
                     KARLTGEGEKARRTAEADALRAEGTAQAAAILATGEAEAQAMNKRADAYARYNEAAVL
                     QMLVEVLPKVARELASPMAAIDNLTVISTDGAGALPKQVTANLVQTMSMLKSATGFDL
                     EDIVKRYSGFVPAPATSVTAATTAPAAPAAETAPPATPATSA"
BASE COUNT         1750 a         4176 c         3906 g         1800 t
ORIGIN      
        1 gacagcggtt ccggttggga cgacagcggt tccggttggg acgacagcgg ttccgactgg
       61 ggcggaggag gcggcggtgg cggctgcggc agcagcgact ggggcgggtg gtccgagtac
      121 tgggccatgc ccgcggccaa cgccagtctg tccaccatgg cctgctactg aggaccgcca
      181 cgcctcaggg cgactgattc ctacaggcca ttgctcgtaa gaaccgcggc gccgaccagc
      241 ggtcggcgcc gcggctcagg acggtgaata gggcaacacg gttcgagagc ccagcacgcc
      301 agccaggggt acccgcaccc agacgatctg ggcgagggcc ggctgggggc catcgcaccc
      361 ggttgccccc acctccggtc cggcccagac cgagatcgcg acggtagaac cggtctccac
      421 ggcctcaatt cccgtcacgc ggtcgcacgg tatgccgccc actacagcct gaacgacgac
      481 ctgcgccgac gtggggtcgg ccttcacagg ctgccacgcc actcgccgaa ctcgttgccc
      541 cgcaacgggg tccgacacca acggcgtgac aatggtcgag ccgggcggct ctggcagcgg
      601 ggccggcgca cccgacggcg gtgccggctg aacctcgcca ccaccaaccc caccagttga
      661 tccaccgacc acggtgcctc ctgtcgaccc cgtcgagccg gggttcagtg tggtgcggga
      721 ctgtccacag ccagccatca agaacagcag cgcgagcacc gaggccgcga cgagcggaac
      781 gggcctgcgg cggtgggcgg acggcatacc gaccacggta ggtgacccca cgcgaacgcg
      841 cactggcgcg cccggggatt ccgggcgcgc caggcgaggg ctgagaacgg tcacagccaa
      901 cgggtggagt tgaacaggct ccacgcatcg tcgtcgaagt agggcgagag catgatcgtc
      961 gggccggaga tccggtcgat ccggctgttg acgccgttga gctggcccca gtcaccgttg
     1021 tagccgtaga gccagccgcc accggacgag ccgcgggtca tgtcacacgg gatcttcacc
     1081 gtctgcgacc agaagaacca ggcgtcccac tcgggtgagg tcgagcccca gcactgcatg
     1141 aggttgccgc cgtcgaacgg ccactcggcc gggtagccga acgcgttctc ccagacaccg
     1201 ctcttgccgc ggttggtcgt caggccctgg ccgccgaagt agtcgacgat gtgccagccc
     1261 cagttggtgt tcatgatcgc gacgcccatg tcctgggaga agtcagagct gttcgtccac
     1321 gcggtcctcg accacaactg gttcgccgac caggtgccgt acggccgcgg gttggccagg
     1381 tcgtcgtcgt aggccggcac gaaggtccag ttggtgtgcc aggtgccacc gctgccgccg
     1441 tgcacgcagt gaccggcagt ccagacggtg tccttgccct cggagttgat gatggttccg
     1501 gagcacacgt agttgccgtc gggcttgctg aagaacacct tgcccgcggt gtaggcggtc
     1561 ggcacgtagt agggcaggtt cgggttgtag gccgccgcga cgccaccagc cgtcgccgcc
     1621 gggccgaaga ccttgctcga cgcaccttca acactgcgga ccggaccgtc gttggtcacc
     1681 ggcttgcggc cggccttctg gtcggcgacc tgcttctgca cgtatgccgt ggcgctggct
     1741 tccagcgtgg aatcctcttc ggcgggcttg gcggctcgca tccgctcggg cgtccaatac
     1801 gcctcaaggg cctgcgcgtc ggtggccttc gcgccgtgct tggcgacgcc ggcggccttg
     1861 gcctcggcgg agatcgacgc cgtggccgac gagacgtcgc cccgggcccc aggcttggcc
     1921 gcctgtgccg acccggcact accgacggcg agcgcggccg cggtggcgaa gacggcaccg
     1981 agggcgagca atcgagttgg gagagtggac tttttcatga tcatccttcg gttggaccca
     2041 ggcaccccgg cactgggttt caaccgtaag agagcccggc gcccccgccc tggatacgcc
     2101 ttcaggtcag gatcttccga accccacacc gaaggcggag gtcagccctc gtcactcgtc
     2161 ggggacgccg gcgaccgggc ggccgtcctc gaggtagcgg cagccgccgg tctccagcac
     2221 cgcagcaacc cgacggctca cggccggccg cagtcgggcc agcgcctcac tcggagcgag
     2281 gaaggcgtgg tcgctgagct cggtcgactg cagcgtgatc gaggccgcct gctcgtcggt
     2341 gaccaccccg cagtcgaaga ggaaccgcag ccccatggcc ctgcgcgcct tggccggtcg
     2401 ggtgtcgacg gcggcgagcc gcccggcgct cacccgcagc cccgtctcct caaagacctc
     2461 acgctggcag gcctcccacg gcgtctcgcc gttggcctcc atgacgcccc cggggatcgt
     2521 ccacccgctc ttgtaggtcg gtttgagcac gaggatccgc ccgcggctgt cgcggatgag
     2581 cgccccggag gaggccggga tccccgggag gccgtcccac gcgcgcaacc ccgcggcata
     2641 cccctgctcg ctgtcctcgt atgccgcgtc gtcggccggc gcgctcgccg ggccaccggt
     2701 cacggggaca tctcctgcgc tcatgcgccc actctaggca ggcgggcgag ggggatgccg
     2761 aacggcggtg cgccgctaca gtggcccggt gaccacgcct cgcttcgcgg ccgccctcaa
     2821 cgcccagatc ggcaacgagt tcgccgccca cctgcagtac gtcgcaatcg ccgtgcactt
     2881 cgacgcgctg acgatgcccc agatggcgtc gttcttctac gtgcatgccc gcgaggagcg
     2941 cgaccacgcg atgatgatgg tccgctacct gctcgacacc gaggccgagg tgacgatccc
     3001 cggtagcgag gcccccatca cgcgcttcga cggggtggtg gagcccatcg agttgtccct
     3061 gagccaggag cgcacggtca ccgagcaggt ctacgcgctg acgcggatcg cccgcgagga
     3121 gaacgactac ggcgcagagc agttcatgca gtggttcatc aaggagcagg tcgaggaggt
     3181 ggcgaagatg tcgtcgctgc tgacggtggc caagcgcaac accgactcca tcgccgacct
     3241 cgaggcctat gtcgccgggc agcaggccaa cgggggcggc gtcgatccga ccgcgccgtc
     3301 gatcgccgac gcctgatcgg gccggggctg ccagggccaa ccggacgccc ttcgcggagc
     3361 gttggccacc ccggggtgtg ggtcacactc cgcgaacccg gtcaccgctg gatgagcccg
     3421 agttcaaggc cacgcaggac ggctcgggtc cggtcacgga cgcccaactt gagcaggacc
     3481 gccgacacgt ggttcttgat ggtgccctcg gcgaggaaca tcgccgccgc gatctcccgg
     3541 ttggagtaac cccccgacag cagccgcagc acctcggtct cacgcgcagt gagcggctcc
     3601 ggcgcgagac cgaccgggtc cacgtcagcc gacgttcccg cgagttgacg cacgagcgcc
     3661 tcggtgaccg acggctggat gattgtgccc ccgccggcga gcgtcgtgat ggcgtcgacg
     3721 agttgatcga ggctgacgtc cttgagcagg tatccgcgag cgccggcgcg catcgccgcg
     3781 atcagggcct cgtcgtcggg gaaggtcgtc aggacgagga ccggggtgcg atcacccgcg
     3841 gcggcgaggg cctgcaggac gcccaggccg tcgaggatcg gcatccgtag gtccaggagc
     3901 acgacgtccg ggcgcagcgc cgcgacgacc tccaggcctt cggcaccatc actggcctga
     3961 ccgatcacct cgacctcgcc ggagagtcgg agcagcccga cgatcccctg gcggacgagg
     4021 gtctgatcat cgatgacgac gacgcggacc gtcatgtcgc agccacccgg cccaccggca
     4081 ccgacgcctg gagggtgaac ccttggccgg gacgcgcggt ccacgccacc gagcctccca
     4141 gtgcctcgaa gcgttccacc atcccggtca ggccgtgccc catgaccacg tccgctccgt
     4201 cacccccgga cccttggccg tcatctcgcg cggtcacgct gaccactccg tcgagctcct
     4261 cgacagcgat ccacaccgag gtggcgccac tgtggcgcac ggcgttggtg acgatctcct
     4321 ggatgcagcg ccgcacgacg tcggcctcgg cggcacccag cggcggcagg tcgtcagcaa
     4381 cgtcgacatc cacgaccggg ctcggcaccg agtgcccgat cgtgcgcaac tcttccgcga
     4441 actccggccc ggcactgggt gtttcgcgca gctgactgac cacctcgcga acgtcacgga
     4501 gcaggtcgga ggcgacggcg cggcatacct cgaggtcgtc cttggccggc ccggtggcgg
     4561 tccgggaggc gacctggagg ttgaccgaca gggccgtgag ttggtggcca acgacgtcgt
     4621 gcaggtcgcg ggcgatccgc agccgctcct gcgtgcgact ggtcgtcgcc aactgcgtct
     4681 gcgcggcccg cagcgccgcg ttggccgcct cggcctcccc gcgagcccgg tcggcctcgt
     4741 tccgcgcggc ctcggtggcc cgcaccgaca cgaccatgag cagcgcgaag agttggaagg
     4801 cgaagaacgc gaccccggtc gacacggcat acgccatccc gccgtcgctg aacgtggtcc
     4861 acaacagagc gaccacggcc agcgtctgga cgaggacgat gccgaccacg acacgcaccg
     4921 acaccaggtt ggccaggagg atcgccgtca tcaccaacga gatcgaggtg aacccgctcg
     4981 aggaggagag cgcgacgacg gcaactccca gcggggtcac cacgccgagt gcgatggccc
     5041 gctcccgcct gggccaatga cccttcggcg ccaccacgac acaccagagg tatgccgcgc
     5101 cgtaaccgag gtaggccagc caccaccagt tcgacgcccg gtccgagagc ccgatgagct
     5161 cgacgacgcc gatcgacagc caggccatcc cggcggtggc gatccccaac ggttcgaggt
     5221 ggcgttcctg catgacgtcg agcgtagggc gggcgacccc gtcggcaccc gtgccggtcg
     5281 tcatgggtgc cggtcatgac gaccggcact gacacagggg tatgccgccc aactaccgtc
     5341 gacgacatga cctccacacc cgcctccggc tcaacgacca cgcctgccgt gatccgtgcc
     5401 agcggcctca ccaaacgcta cggcgacctc gtggccgtcg atgccctcga cttggtcatc
     5461 gcgcccggcg acatcgtggc gatcctcggc cccaacggcg caggcaagtc gacgaccatc
     5521 gagatgctgc tcggcctgcg cacccccacg gccggggagg tccgtgtctt cggcgacgcc
     5581 cccaacgcga aggccactcg ggctcgggtc ggcgcgatgc tccaagagag cagcgccccc
     5641 gaaagcctca ccgtggccga ggcggtcgat ctggtgcggc actactaccc gttcgccctg
     5701 agcctggacg acatcctcga ccgcgccgac ctgtcggaga agcgcgggaa ccggatcgcg
     5761 caactctccg gaggccagca gcaacgcctc aacttcgcct tggccatcgc cggcgacccc
     5821 gacctgctct tcctcgacga gccgaccgca gcactggacg tcgaggcgcg acacctgttc
     5881 tgggagcagg tgcggggctt ggctgcgcac ggcaagacga tcctcttctc cacccacaac
     5941 cttgccgagg ccgatgccct cgccgaccgg gtgatcctca tcaaccgcgg ccgagtcatc
     6001 gccgacggca cgcccgagca gatcaaggcc acggtcgcga cgcgaaccgt ccgactcgtc
     6061 accgacgtcg gcgtcgcggc gttggccgcc ttccccggcg tccgcggggt cgaggtcgct
     6121 ccgggcgagt ccgtgggtgc cacgcacccg accctggtcg tgcagaccgc cacgcctgag
     6181 gacctgctgg ctcgactctt cgccggtggc cacctggtgt cgcagttgac cgtcaccgac
     6241 accgacctcg agacggcctt catccacctc gtcggtgcca ccacccagac cgcaccgacc
     6301 gccacccccg acaccacccc cgagcacgtc atggagtccg catcatgagc accgccggcc
     6361 gcacggcata ccccgccgac tcgtcgacca cggacagcgg tgaccgcacc ccccgacggc
     6421 tgagccccga agcatcccgt atgccgggtg ccctcggcac gttggccgcg cagctgcgcg
     6481 ccgaactcct ttccagctca agggttcccg agtacgtcgt cggggtggtc gccgtgccga
     6541 tcatcctctt cacaatgttc gggctgccca acgcgggggt aaaactcccc ggcgggaccg
     6601 atgtcggggc gatgatgttc gcctcgatgt ctgcctatgg cgtcgtcagc ctggcgatct
     6661 tcaccttcgg ggtgggcgtt gcggacgagc gcggcaaggg ctggctgcgg cggatgcgcg
     6721 cgacgccgat gccgttctgg gcctatttcg tcgccaaggt cgtcggctcg ctggcgttct
     6781 gcgggctcat cctgctcggg acctgggcgg tggctgcgct gggcggcaag gtgccgttcg
     6841 acggcgtgcg gctcgctcag accgtgctca tcctgctggc cgggacggtg gcgttctcga
     6901 ccatgggttt ctcgctggcc tattggttcc gccccaaggc ggcgtcagcg atcggcaatc
     6961 tcgtcttcct gccactgtcg ttcctctccg gcttcttctt ccccctcgac caactccccg
     7021 acgtactggc caaggtggcg ccctatctgc cgacctacca cttcgggcaa ctcgtctggt
     7081 cggcgatcgc cccggcgcag gacgtcatcg ccttcggcgc ccatcctgaa ggcagtgcct
     7141 gggtgcacgt cggctgggtc ctcgcgtcgt tcgtcgggtt cggcctgctg gctcgcgccg
     7201 gctaccgccg cgagttgtcc cgcgccgccc agtagtgcgg cgggcagcaa gcgccggggc
     7261 agcagcgcgg cgggcggcac tgtgcgagcg ccgtccgtgg caggctgaag gcatgaccga
     7321 gacccccgcc gcagccgctc ctgtcggtga cgacctctcc ctgtcgcacg ccctgctcat
     7381 cgacgccttc ggtcgggtcg ccgagggcgt ccccgccctc ctcgacggcc tcggcggcga
     7441 agagatcctg tggcggcccg acgaggactc caactccatc ggctggctcg tctggcacct
     7501 cgcccgagtg caggatgacc acctcgcggg agtggccggg cgcgaacaga tctggacgac
     7561 agccggtttc gccaagcgct tcgacctccc gtatgccgcc cgctcaatcg ggtacggcca
     7621 gtccagccag gaggtcgcgg ccttctcggt cgccgacggg agcctgctcg cggcatacca
     7681 tgcggcggtc cacgccgaga cggtggagat cgtgcagggt ttgaccgcag ccgacttcgc
     7741 gcggatcgtc gaccggcgct gggacccgcc ggtcaccgcg gccgtgcgcc tggtgtcggt
     7801 cgtcaacgac atcacccagc acgccggaca ggccgcctac ttgaggggtc tggtcgaacg
     7861 tcgccgcgcg tgagcgcggg cggggacgtc gcgggggtat gccgttcgcc tcaggactac
     7921 gcgaggggcg gcaaggactc ctaggctgcc taggtgctca ccgcgttcgc cgtcctgctg
     7981 tgtctgtcgg cactcctcgc ctgggccaac gaacgttggg ttcacctgcc cagcacggtg
     8041 gccgtcaccc tcgcgggagc cgcctcatcg atcgtgctca tcctgctcga cacgcaaggc
     8101 tggaccttcg ggctgaagca gcaggcgatc gagctctggc aggcgctgga cttcaccgct
     8161 ttcgtcctca acggcatcct cagcctgatg ctcttcgcgg gagcgatgag cctggacgcc
     8221 acactgatga tgcgcctgcg ggccggcatc ctcaccttcg ccggggtgag caccgcgatc
     8281 agcaccgtgc tcatcggctt cggcgcgtgg ggcgtcttcc acctggtcgg gctcgacatc
     8341 ccgctcatct ggtcgctgct cttcggtgcg ctgttgtcgc cgaccgaccc cgtggccgtg
     8401 ctcgacatgc tcaagcgcgc caaggtgccc aagaagatcg agaccctcat cgccggggag
     8461 agcctcttca acgacggcgt cggcgtcgtc ttcttcatcg tcattgcctc cgccgcgggg
     8521 gtcggtggcg gccacaccga gcccagtctc acgggagcgt tggtcgtctt cgtccaagag
     8581 gcgttgggcg gcatcgcctt cggcgccctg atcggctgga tcacctatcg gctgtgtcgc
     8641 tcgatcgagg cggcgtcggt cgagatcctg ctgaccctgg cttcggtcgt cggcggctat
     8701 gtcctcgccg cgagcctcgg cctgagcgga cccctggcga tggtcgtcgc cggcctggtg
     8761 atgtcggccg gcaaagagcg agcgttcgga gagcacaccc gggaacggat cgaaggcttc
     8821 tgggagaccc tcgacgaggt cctcaacatc ctgttgttcg cgttcatcgg tctcgacgtg
     8881 ctgctcacgc ctaccgccgt cggcgaagtg ctcgccgggc tcctcatgat cgtcgtggcc
     8941 ctcgtcgcgc gatttgtcag tgtcgcgctg ccgatgaccc tggtccgccg ccgggacggc
     9001 tacgggccgt ggacgattcg ggtgctcacg tggggaggcc tgcgaggcgg catcgcgatc
     9061 agcctcgccc tcgggctccc ggccagcccc taccggtccg ctctggtcac cgccacctac
     9121 gtcgtcgtcc tcttcacgat cgccgtccag ggcctgaccg tcatgccgat cgtccgtcgc
     9181 gccgcggcgg ccgaggcggc ggccgaggcg gcggcgggcg agactgagaa tcgcgctgct
     9241 cgtccgtgac gtcaggctga ggcacgtcgt tttcgcgggt gaccacgatc ggcgtcgact
     9301 cgtggaaccg aatgcaccgg cataccatcg aagaagacgg tcgggacagg agggccatgc
     9361 tgaccgtatt cctcgccatc ggcgccgttg gtttggcgct gctcgtgatt tcgctggtcg
     9421 tcggcgagat tcacggcggc cttttcgagt ggatcgacaa cgaccttttc tccggcgccg
     9481 cggtgtcggc cttcctgtcc gcgctcggtt tcgtcggcgc gctcgtcgaa agcacctcgg
     9541 gcaacaccac cgtcgcgact ctcgcgggca cgggggccgg cgtgggcatg gcgtatgccg
     9601 cccgcggtct cacgcgctcg ctgcagtccg gcgagggaga caccacggtg cgttccggcg
     9661 acctcgtcgg ccaccgaggt catgtcgtcg aggcgattcc ccgcgatggc atgggcatgg
     9721 tcagcgtgag cgtcgctggg cacctcaccc ggctcaacgc ccgctgcgaa cagccgctct
     9781 ccgcgggaac cggaatcgag gtcacctccg tcctatcccc gacggcagtg acggttcgac
     9841 ccattgtcgt cacccctcag gatcgcgcgc tcgactgagc gcgcatagcc gccggaaagg
     9901 cgcgaacaat gtctgccaat gagtttcctg tcatcctcgt tgccatcgtc gggctgatca
     9961 tcctgctcgt cgtggtgggg gtgctcgtcg cctcgcgcta caaggtggcc gggccaaacg
    10021 aggccttcat cgtcaccgga cgcaagggca aggagatccg cgacgtcgaa accggtgagg
    10081 tctccaccga tctgtccggg cagaaggtcg tcatgggtgg cggcattttc gtcgtgccgt
    10141 tcgtccagcg cctgcacgtc ctggacctct ccagccgccg gatcatgatc cagatccgcg
    10201 gcgccgtctc gggccagggc gtcaagctca acctcgacgg ggttgccctc gtcaaggtcg
    10261 gcggcaacga agactcgatc cgcgcggcgg cccaacgctt ccttacccag cagcgcgaga
    10321 tcgagacctt cacgcaggag accctggcgg gctcgctgcg ctcgatcgtg ggttcactga
    10381 ccgtcgagca gatcatccgt gaccgggccg ccttcgccca gcgagtggcc gacgagtcgg
    10441 agacctcgct taccggtcag ggtctggtcc ttgacacctt ccagatccag gacatcaccg
    10501 acgacgggag ctacctctcc gacctcggcc gccccgaggc cgccagggtt gggcagacgg
    10561 cggcgatcgc cgaggccaac gcccgacagg ctgcggagca ggctcggctg gccgccgagc
    10621 aggagatcgc catagccaac cgtgagttgg ccctcaagca ggccgagatc aaggcgcaga
    10681 ccgacgcggc caccgctcag gctgccgccg ccggccccct ggctcaggcc gaccgcgacc
    10741 aggcgatcct tgccgagcag gagaaggtcg ccgtgcgcaa ggccgcgctc accgagcgcc
    10801 agttggacac cgaggttcgc cgcccggccg acgccgcccg gtatcgcgtc gagcaggagg
    10861 ccgacgcccg caaggcggcc tcgatcgccg cggcccaggc cgacgccgag aaggcccggc
    10921 tcacaggtga aggcgagaag gctcggcgca ccgccgaggc cgacgccctg cgagccgaag
    10981 gcaccgccca ggccgccgcg atcctcgcca ccggcgaggc cgaggcccag gcgatgaaca
    11041 agcgcgccga cgcctacgcc cgctacaacg aagccgccgt cctgcagatg ctcgtcgagg
    11101 tcctgcccaa ggtcgcccgc gagctcgcct cgcccatggc ggcgatcgac aacctcaccg
    11161 tcatctccac cgacggcgcc ggggccctgc ccaagcaggt gacggccaac ctggtccaga
    11221 ccatgtcgat gctcaagagt gcgaccggtt tcgacctgga ggacatcgtc aagcggtact
    11281 ccgggttcgt cccggctccc gccacgtcgg tgacggcggc cacgacggcc cctgcggcac
    11341 cggcggccga gaccgcgccg cccgccacgc ctgccacctc cgcctgacgg gcgcaggcta
    11401 ggggctcgcg ctcgagttct ttctgctcga gccgggtcct ggtcgcccgg gcggacgctg
    11461 cggatcgtta cccgtttgtg ttgcgtttgc ctgtggaagc ggcggtttgg ggtgggtggc
    11521 ctgtcggtgg tcgctgttag cgtcggggtt atggacagca gcgaagcggc tcgtgattcg
    11581 gcatctcgcc gggctgagat tcgtgctgcg acgaggaccg tcgctgagtt gg
//
LOCUS       DAOMZY010000065        11560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5225_length_11560_cov_5.513081, whole genome shotgun sequence.
ACCESSION   DAOMZY010000065 DAOMZY010000000
VERSION     DAOMZY010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 11560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11560
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5225_length_11560_cov_5.513081"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..1226
                     /locus_tag="PLK64_05365"
     CDS             21..1226
                     /locus_tag="PLK64_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPV79202.1"
                     /translation="MGLVVEVPGRKRVFVPTTRVTSLEESQVITTGVVNLRRFEPRAG
                     EVLAIAGLRERRVIVRLAPGSRTAEATAAQSTNTQSTNTRDADARPSIDATVEDLAVD
                     IRPKAWTVTKVYVRKLVHAPRRGLHLNRRRGETLLVDIADVSGLDTDPRESDTAALLA
                     AYRDGAVADLADAIHDLARDRRRQVATALDDDRLADVLEQLPEDDQVEILAGLDDERA
                     ADVLEAMEPDDAADLLAELPAAHREGLLARMEPDEAEPVRRLMAYDEHSAGGLMTSEP
                     VILPPEATIAEALALVRREEVSTTLATTVFVCRPPLETPTGRYLGIVHFQKLLREPPH
                     AAVGSVLDKVKPLHPASTVDHVHRMFATYDLMAAPVVDGHGRLVGAVTVDDVLDHILP
                     EDWREGTHG"
     gene            1219..1848
                     /locus_tag="PLK64_05370"
     CDS             1219..1848
                     /locus_tag="PLK64_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009357714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HPV79203.1"
                     /translation="MADRRRPNRTPGRGRGARLDQPREFRRRDFLPGPGFSQESFGVW
                     SEQFARFMGTPLFLIGLTAFAVIWIGWNTWAPVAWQFDPRELNYTLLTLLLSLQASYA
                     APLILLAQNRQSDRDRVTVEQDRARDERNLADTEYLTREVAALRLALREVATRDFVRS
                     ELRALLQELDERAVDERTVDERTVDERATTEPSSSSPRLPSGTGERRSD"
     gene            1876..3018
                     /locus_tag="PLK64_05375"
     CDS             1876..3018
                     /locus_tag="PLK64_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPV79204.1"
                     /translation="MSLVALPTQDALLAALAKVNDPEIRRPVTELGMVKSVSVDDAGH
                     VALELFLTVAGCPMKDTLRKDTSAALLRVPGVVSVDVALDVMSDEQRAALRTQLRGGV
                     AEREIPFAKSGSLTRVYAVASGKGGVGKSSVTVNLAAALAAEGLKVGVVDADVHGFSI
                     PRMLGVTGLPTQVDDMILPPISHEVKVISIGMFVPGNQPVVWRGPMLHRALQQFLGDV
                     FWGDLDILLLDLPPGTGDIAISIGQLLPTAEILVVTTPQQAAAEVAERAGSIALQTHQ
                     RVVGVVENMSWLELPDGSRQEIFGSGGGKTVADSLTRLIGAPVPLLGQVPLDAGLREG
                     GDSGTPIVLSDPDSPAAVVLRAVAKGLAQRSRGLSGRSLGVSPVKR"
     gene            complement(3060..3422)
                     /locus_tag="PLK64_05380"
     CDS             complement(3060..3422)
                     /locus_tag="PLK64_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sec-independent translocase"
                     /protein_id="HPV79205.1"
                     /translation="MFDINGWELIVLAMVAVVVLGPERLPVYAAKLARAVRQVRTLAE
                     GARSQLKEQLGPDFDDVNWRQYDPRQYDPRRIVREALMDPAEPAAQAEARVWAPEEQP
                     GRAAYDPGKPTPWDSEAT"
     gene            complement(3468..4823)
                     /locus_tag="PLK64_05385"
     CDS             complement(3468..4823)
                     /locus_tag="PLK64_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPV79206.1"
                     /translation="MSEHPDRPAFDPGAGSAPASWPDPASDPRSGVPTAALPAYPPAA
                     AAPPPSGYPPPSVYPPPPSAGVFPPAYAVPAPPAPASAAASVPATAARQPGSRVGLMV
                     ALAAVVGLLAGLLGGGVGSVLVNRGVAVVQGQSAVPAPLPGVTARPDGSIAKIAATAL
                     PSVVTVKIRTASASGNGSGFVIDRAGHILTNNHVVSGATGPGEITVEFVDGTSVAARI
                     VGRDSSYDLAVLAVDKTDVTPLEWGRSSDVVVGDGVIAVGAPLGLEATVTSGIVSALN
                     RPVVATGGDDMSFINAIQTDAAINPGNSGGPLLDMSGRVIGINSAIARDPGSTSSSGG
                     SIGVGFAIPSDQAAKTAQQLITTGKAEHPIIGVKLDTQFTGEGARIADPGGVTPGSAA
                     DTAGLRDGDVIVRFEGKKVPGSNQLVVAIRAKSVGDTVHLTVERAGAQMDITLTLRAS
                     N"
     gene            complement(4820..5425)
                     /locus_tag="PLK64_05390"
     CDS             complement(4820..5425)
                     /locus_tag="PLK64_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79207.1"
                     /translation="MSRHLGDQVSAYVDRRLPGSALSAWDRHVVVCAHCRYAVDQERS
                     LLASLRTAPAPCVSDSLQALLLGMGNASPNARFIAAAASTGSPVGAQPPVPAAPFLVH
                     GLRLPTVSPAGPPRHRSARRAALVAGLAAGASAAAAWTVGLAPASVSAAPAGAPVPAR
                     VGTASGLGAAYSGMLSFPVSQVGFSTVWPTSTTSTTEISGR"
     gene            complement(5425..6099)
                     /gene="sigE"
                     /locus_tag="PLK64_05395"
     CDS             complement(5425..6099)
                     /gene="sigE"
                     /locus_tag="PLK64_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigE"
                     /protein_id="HPV79208.1"
                     /translation="MIRPLTGSDTVPDEGSGWTPPTWEEIVTQHSARVYRLAYRLTGN
                     PHDAEDLTHDVFVRVFRSLNSYTPGTFEGWLHRITTNVFLDKMRRKQRIRFDALPDDA
                     ASRMASREPGPEQVFTDTHFDDDVQRALDALAPEFRAAVVLCDIECLSYEEIAATLGI
                     KLGTVRSRIHRGRAALRESLAHRAPGADGAAARVTTPAPAASRPAARHGIPLPSRLRP
                     AARAGA"
     gene            6321..6953
                     /locus_tag="PLK64_05400"
     CDS             6321..6953
                     /locus_tag="PLK64_05400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HPV79209.1"
                     /translation="MSANRAASWAYAEQFIAEGPTMEAARERGQALGAVPVGVATGAA
                     LRMLAATGAAKAVVEIGTGAGVSGLWLLAGMPSDGVLTTIDVDADHQRAAKEAFAEAG
                     IAHQRTRVILGRALDVLARMTDAAYDLAFVDGDKDEYPAYVREATRLLRPGGVLALDN
                     MFWHDQVADPTARDERTRTLRDLGKALREDDAFASALLPVGDGLLVAVKR"
     gene            complement(7025..7198)
                     /locus_tag="PLK64_05405"
     CDS             complement(7025..7198)
                     /locus_tag="PLK64_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3117 domain-containing protein"
                     /protein_id="HPV79210.1"
                     /translation="MAAMKPRTGDGPLEVTKEGRGIVLRMPLEGGGRLVVEMTPDEAA
                     ALRDAIAGCDGVS"
     gene            7331..8140
                     /locus_tag="PLK64_05410"
     CDS             7331..8140
                     /locus_tag="PLK64_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPV79211.1"
                     /translation="MTDDRAAQTAPVLLHVEGGVATVTLNRPEAMNSLDLATKDLLVP
                     TIRQVADDPAVRVVVITGSGRAFCVGQDLKEHIAGLMAGDEGLGVTVVKHYNPIAEML
                     ATMNKPVIAALNGVAAGAGASIAMAADFRIMVEGGGMNLAFAGIALSCDTGSSFWLPR
                     LVGVAKAKELLLLPRTLSAQECLDLGLVTSVVPAEAFAGEVATLAGKLAAGPTLAYGA
                     MRQAIAFSAAHSLTESLAFESDLMNSTGQTADHRVAVDAFLAKQKPVFEGR"
     gene            complement(8153..9490)
                     /locus_tag="PLK64_05415"
     CDS             complement(8153..9490)
                     /locus_tag="PLK64_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79212.1"
                     /translation="MGVARGRRSVGRPLDASPASVAAAAAAADSAPDSKAALSGPPFA
                     RAEAVLDRVFRGNAVVQVVVLYAITRLLTTVMLALVAPSQLPADMTDHQAVDYVGFTR
                     LWDGQWYERVATGGYPNTVPRDPAGIAMQNTWAFYPLFPFLARAVMEVTGLSFAAAAS
                     TLALVIGFGAAVAMAALLRPRIGRAGALLVVGLYAVFPSSPALQVAYTESLAMLLLCG
                     YLLALSRERWLVAAGLALLIGVTRPIALPLGVVTLVAVWLRWRRRAAEPMSPGETGAA
                     LTSLVGCGVAGLLWPGIAWVATGQASAYFDTMGAWSMTGHVQILEPWFTIPRYYLGDW
                     GPRLFAVTLVLLLVGMAGPWARRLGPELRVWPVIYAAYVIAVQTPGTSTARYLLPMFP
                     YLAVLLGLASERGRARGIPTTVRALVLGAAFLWLQWKWVSVVWLFTPPIDFAP"
     gene            complement(9501..10115)
                     /locus_tag="PLK64_05420"
     CDS             complement(9501..10115)
                     /locus_tag="PLK64_05420"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HPV79213.1"
                     /translation="MSATAVVTGPDGIPRCAWAASTADYVAYHDDEWGVPLHGERELF
                     ERLTLEAFQSGLSWLTILRKREGFRAAFAGFDPDVVAAFDAGDRARLLADTGIVRNVR
                     KVDAAIHNAQAVVDLRDDGGLDALVWAHAPAHHDRPQGMGDVRATSPESVALTKALKA
                     RGFVFLGPTTMYAAMQACGLVDDHLAGCHRAGGQQARPAAPEPR"
     gene            complement(10112..10546)
                     /locus_tag="PLK64_05425"
     CDS             complement(10112..10546)
                     /locus_tag="PLK64_05425"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPV79214.1"
                     /translation="MTAVTIQRVIPRPIEAVWAVVSDLEAHPVTLTRIDTDAGPPRVG
                     WAFVALTGIGRLRFADRMVVTRWSPPADGRAEFAIVKTGRWLGGWATIRLEALDGTRT
                     RLTWREEIVPHPHAIGRLAAPLTDRAVRSIFTRAVAEMAARA"
     gene            complement(10550..10936)
                     /locus_tag="PLK64_05430"
     CDS             complement(10550..10936)
                     /locus_tag="PLK64_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HPV79215.1"
                     /translation="MWIALVVAVVLVGLTVAAIFGRVDGSLVDPTSSLSYAPLPDGPL
                     SPDDVQGLRLDTGLRGYRMEQVDEVIDRLTAEITVLRDQIDAATTAAYQPGPDADPSM
                     PPASPFVRPSEWAEFSEPNARPDSQA"
     gene            complement(10936..>11560)
                     /locus_tag="PLK64_05435"
     CDS             complement(10936..>11560)
                     /locus_tag="PLK64_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HPV79216.1"
                     /translation="PWRVMRIQSEFVEGFGALAELGPAVSVFGSARLGAGHPAYELGV
                     QVGRRLVASGYAVITGGGPGLMEAANKGALEAGGTSVGLGIELPFETGLNDYVDLGVN
                     FRYFFARKTMFVKYAQGFIVLPGGMGTLDELFEALTLVQTGKITTFPIVLLGRDYWAG
                     LIDWFRTTLVAAGTISPDDVDRILVTDDVEQAVRFVVDQDHLHTEVR"
BASE COUNT         1770 a         4033 c         4114 g         1643 t
ORIGIN      
        1 cgccgaccac gccccgggtc atcggcttgg tcgtcgaggt tcctggccgc aaacgggtgt
       61 tcgtcccgac gacccgggtg acctcgctgg aggagagcca ggtgatcacg accggcgtgg
      121 tcaacctgcg ccggttcgaa ccccgcgccg gagaggtgtt ggccatcgcc ggcctgcgcg
      181 aacgccgggt gatcgtccgg ctcgcaccag gcagccgcac cgccgaggca acagccgccc
      241 agtcgaccaa cacccagtcg accaacaccc gcgacgcgga cgcccgcccg agcatcgacg
      301 ccaccgtcga ggacctcgcc gtggacatcc ggcccaaggc atggaccgtg acgaaggtgt
      361 acgtccgcaa actcgtccac gcgcccagac gcgggctgca cctcaaccga cgcaggggcg
      421 agacgctcct ggtcgacatc gccgacgtct cgggcctgga caccgacccg cgcgagagcg
      481 acacggcagc gctgctcgcg gcataccgtg acggagccgt cgccgacctc gccgatgcga
      541 tccacgatct ggcccgcgac cggcggcggc aggtcgcgac cgcgctggac gacgatcgcc
      601 tggccgacgt cctcgagcaa ctccccgagg acgaccaggt ggagatcctc gccggcctgg
      661 acgacgagcg ggccgccgac gtcctggagg cgatggagcc cgacgatgcc gccgacctgc
      721 tcgccgagct cccggcggcc caccgcgagg ggctgctggc caggatggag ccagacgagg
      781 cggagccggt ccgacggctg atggcctacg acgagcacag cgcgggcggc ctgatgacca
      841 gcgagccggt catcctcccg cccgaggcga cgatcgccga ggcgctggcc ctcgtccgac
      901 gcgaagaggt ctcgacgacg ctcgcgacga cggtcttcgt ctgccgccca ccgttggaga
      961 cgcctaccgg tcgttacctg gggatcgtcc acttccagaa gctgctgcgt gagccgccgc
     1021 atgccgcggt cggctcggtc ctggacaagg tcaagccact gcatccggcc agcaccgtcg
     1081 accacgtgca tcggatgttc gcgacctacg acctcatggc agcgccggtg gtcgacggcc
     1141 atggccgtct tgtcggcgcg gtgacggtcg acgacgtcct ggaccacatc ctccccgagg
     1201 actggcggga gggcacccat ggctgaccga cggcgcccca accgcacccc gggacgcggt
     1261 cgaggcgccc ggctggacca acctcgggag ttccgccggc gcgacttcct gccgggaccc
     1321 gggttctcgc aggagtcctt cggcgtgtgg agcgagcagt tcgcccggtt catggggacg
     1381 ccgctgttcc tcatcggtct cacggccttc gccgtcatct ggatcggctg gaacacctgg
     1441 gcgccggtgg cgtggcagtt cgacccgcgg gagctcaact acacgctgct caccttgctc
     1501 ctgtcgctgc aggcgtcgta tgccgcgccg ctcatcctgc tcgcccagaa ccgtcagtcc
     1561 gaccgtgacc gggtgaccgt cgagcaggac cgggcccgtg acgaacgtaa cctcgccgac
     1621 accgagtacc tcacccgcga ggtggccgcg ctccgattgg ccctgcgcga ggtcgcgacc
     1681 cgcgatttcg tgcgctcgga gttgcgcgcg ctcctccagg agttggacga gcgcgcggtg
     1741 gacgagcgca cggtcgacga gcgcacagtg gacgagcggg caaccaccga gccgtcgtcc
     1801 agcagcccgc ggctgccgtc gggcactggt gaacgtcggt ccgactgacc cgatcagcca
     1861 cctacgatgg agcccatgtc gctcgtcgct ctccccactc aggatgccct cctcgctgcc
     1921 ctcgccaagg tcaacgatcc cgagatccgc cgaccggtca ccgagttggg catggtcaag
     1981 tcggtgtcgg tcgacgacgc cgggcatgtg gccctcgagc tcttcctgac cgtcgccggc
     2041 tgccccatga aggacaccct gcgcaaggac acgagcgccg ccctcctgcg cgtgccgggc
     2101 gtggtgtccg tcgacgtcgc cctcgacgtc atgagcgacg agcagcgcgc ggccctgcgc
     2161 acccaactgc gcggcggggt cgccgaacgc gagattccct tcgccaagag cggctccctc
     2221 acccgggtgt atgccgtggc ctccggcaag ggcggcgtcg gcaagtcgtc ggtcaccgtc
     2281 aacctcgccg cagccctggc cgccgaggga ctcaaggtcg gcgtggtcga cgccgacgtc
     2341 cacggcttct cgatcccgcg gatgctcggt gtcaccgggc tccccaccca ggtcgacgac
     2401 atgatcctgc cgcccatctc gcacgaggtg aaggtcatct ccatcggcat gttcgtcccc
     2461 ggcaaccagc cggtggtctg gcgtggcccg atgttgcacc gggcgctgca gcagttcctc
     2521 ggcgacgtct tctggggtga cctggacatc ctcctgctcg acctgccgcc gggcacgggt
     2581 gacatcgcca tctccatcgg gcagttgctc cccaccgccg agatcctcgt cgtgaccaca
     2641 ccgcagcagg ctgcggcgga ggtcgccgag cgcgccggct cgatcgcctt gcagacccac
     2701 cagcgggtcg tcggggtcgt cgagaacatg tcgtggttgg agcttcccga cggatcgcgt
     2761 caggagatct tcggatcggg cggcggcaag accgtcgccg actcactcac ccgcctcatc
     2821 ggcgctccgg tgccgttgct cggccaggtg ccgctcgacg ccggtctgcg cgaaggcggc
     2881 gactcgggta ccccgatcgt gctcagtgac cccgactccc ccgccgcggt cgtcctgcgc
     2941 gcggtcgcca aggggctggc ccagcgctcc cgcgggttgt caggtcgctc actcggcgtc
     3001 agcccggtca agcgctgact cagccgtgcc ccggcggcct cctcgcgggg gccgaccggt
     3061 caggtcgcct cagagtccca gggggttggc ttccccgggt cgtatgccgc cctcccgggc
     3121 tgctcctccg gtgcccagac gcgggcctcc gcctgggcgg ccggctcagc cgggtccatg
     3181 agcgcctcgc ggacgatgcg ccgagggtcg tactgccgag ggtcgtactg ccgccagttg
     3241 acatcgtcga agtcggggcc gagctgttcc ttgagttgac tgcgggcccc ttcggccagc
     3301 gtgcgcacct ggcgcactgc gcgggcgagc ttggcggcat acaccgggag gcgttcgggg
     3361 ccgaggacga cgacggcgac catcgccagg acgatgagtt cccagccgtt gatgtcgaac
     3421 atctgcccgc gcctcccttc cgccgcccgc cgtgggtcgt tgtgcgccta gttggatgcc
     3481 cgcaacgtca gggtgatgtc catctgggcc ccggcccgct cgaccgtgag atgcactgta
     3541 tcgccgacgc tcttggcccg gatggccacg accaactggt tcgacccggg gaccttcttg
     3601 ccttcgaagc ggacgatcac gtctccgtcc cgcagaccgg ccgtgtcggc ggcgctgccc
     3661 ggggtcactc ccccagggtc ggcgatacgg gcaccctcac ccgtgaactg agtgtcgagc
     3721 ttgaccccga tgatcgggtg ctcggcctta ccggtcgtga tgagttgttg ggcggtcttg
     3781 gcggcctggt cgctggggat cgcgaatccg acgccgatgc tgccgccgct gctcgatgtc
     3841 gagccggggt cgcgggcgat cgctgagttg atcccgatga cccggccgga catgtccagc
     3901 agcggcccgc cggagttacc ggggttgatc gcggcatccg tctggatcgc gttgatgaag
     3961 ctcatgtcgt cgccgcccgt ggccaccacg ggacggttga gggccgagac gatcccgctg
     4021 gtcacggtcg cttccagacc caacggcgca cccaccgcga tgaccccgtc gcctacgacg
     4081 acatcggacg agcggcccca ttccaggggc gtgacgtcgg tcttgtcgac cgccaggacg
     4141 gccaggtcgt aggacgagtc acgcccaacg atccgcgccg cgaccgacgt gccgtcgacg
     4201 aactcgacgg tgatctctcc gggaccggtg gcccccgaga cgacgtggtt gttggtgagg
     4261 atgtgtccgg cccgatcgat gacgaagcct gacccgttgc cggacgcgct cgccgtgcgg
     4321 atcttgacgg tgaccaccga gggcagggcc gtcgcggcga tcttggcgat gctgccgtcg
     4381 ggccgagccg tcactccggg cagcggagcg ggcacggccg actgcccttg aacgacggcc
     4441 acgccacggt tgacgagcac gctgccgacg cccccgccga gcagtccggc cagcaggccc
     4501 acgacggcgg cgagcgccac catgaggccc acccgggagc ccggctggcg cgccgcagtc
     4561 gcagggaccg acgcggctgc ggaggccggt gcaggcggcg cggggacggc atacgcggga
     4621 gggaagacgc cagcgctggg aggcggcgga tacacggaag gtggcggata cccggaaggc
     4681 ggcggtgcgg ctgcggccgg cggataggcc ggcagggctg cggtcgggac gccgctgcgc
     4741 gggtcggacg ccgggtcggg ccaggaggca ggggcagaac ccgcgcccgg gtcgaacgcc
     4801 ggacggtcgg ggtgctcgct catctgccgg agatctccgt cgtgctcgtc gtggaggtgg
     4861 gccacacggt ggagaagccc acctggctca cggggaagga cagcatccct gagtatgccg
     4921 cgccgagacc actcgcggtg cccactcgcg ccgggaccgg cgcaccggcc ggagcagcgc
     4981 tgaccgatgc gggagctagc ccgacggtcc aggcagcagc ggccgaggct ccagcagcca
     5041 gcccagccac gagggcagcc cggcgggccg agcgatgccg gggcgggccg gccggggaca
     5101 ccgtcggcag tcgcaacccg tggacgagga acggcgcggc cgggaccggg ggctgggctc
     5161 cgacgggaga ccccgtcgag gccgcagccg cgatgaagcg ggcgttcggg gacgcgttgc
     5221 ccatgcccag cagcaacgcc tgcaggctgt cggacacgca cggcgccggc gcggtgcgca
     5281 gggaggcgag caggctgcgc tcctggtcga cggcataccg gcagtgtgcg cagacgacca
     5341 cgtgccgatc ccacgccgac aacgccgacc ccgggagccg gcgatcgacg taggcgctga
     5401 cttggtcgcc gaggtgtcgg gacatcacgc tcctgcgcgg gcggccgggc gcagccggct
     5461 gggaaggggt atgccgtgac gcgcggccgg tcgggacgcg gcgggagcgg gggtggtcac
     5521 tcgagctgcc gcaccgtcgg cgccaggcgc ccggtgcgcc agggactcac gcaacgcggc
     5581 gcgaccgcgg tggatgcggg agcggacggt gccgagcttg atccccaggg tggcggcgat
     5641 ctcctcgtag gacaggcact cgatgtcaca caggacgacg gccgcacgga actcgggcgc
     5701 caaggcgtcg agcgcgcgtt ggacgtcgtc gtcgaaatgg gtgtcggtga agacctgctc
     5761 gggccccggc tcgcggctgg ccatgcggct ggcggcgtcg tcgggcagcg cgtcgaagcg
     5821 gatccgctgc ttgcggcgca tcttgtcgag gaacacgttg gtcgtgatcc ggtgcagcca
     5881 gccctcgaag gtgccgggcg tgtaggagtt gagcgatcgg aagacccgga cgaacacgtc
     5941 gtgggtgagg tcttcggcgt catgggggtt gccggtgagg cggtaggcaa gccggtagac
     6001 gcgggctgag tgctgcgtga cgatctcctc ccacgtcgga ggcgtccaac cggaaccctc
     6061 gtcggggacg gtgtcgctcc cggtcagggg acggatcagg gtgtcgctca ctacctcgcg
     6121 cctttcgcag ccttggactt ctgacgttcg tgacacctca agagttccct cgatgcccac
     6181 gagtcgactg tgccctgcct gtgtattggc caaccatccg ggtcgaccct ggtattccac
     6241 ccatccgccc ctgaggtctc gacgccgcaa ggtccgcgtg ctccctgcgt cgaggactct
     6301 cgcgggctag ggtttcgacc atgagcgcca atcgtgccgc cagttgggcg tatgccgagc
     6361 agttcatcgc tgagggcccc acgatggagg ctgcccgcga gcggggccag gctctgggcg
     6421 ccgtgcccgt cggcgtcgcc accggcgccg cgctgcggat gctcgcggcc acgggggccg
     6481 ccaaggccgt cgtcgagatc gggacggggg ccggcgtctc ggggctgtgg ctgctggccg
     6541 gtatgccgtc ggacggggtc ctcacgacga tcgacgtcga cgccgatcac caacgagcgg
     6601 ccaaagaggc gttcgctgag gcggggatcg ctcaccaacg cacccgggtg atcctggggc
     6661 gggcgctgga cgtgctcgcc cggatgactg acgcggcata cgacctcgcg ttcgtggacg
     6721 gcgacaagga cgaatatccg gcctacgtgc gcgaggcgac ccgcttgctg cgccctggtg
     6781 gggtgctggc cctggacaac atgttctggc acgaccaggt ggctgacccg acggcccgtg
     6841 acgagcgcac ccggacgctg cgcgacctgg ggaaggccct gcgggaggac gacgcgttcg
     6901 cctcggcgtt gctgccggtc ggagacgggc tgctggtcgc cgtcaagcgc tgacggcgag
     6961 gcccgaaaac accaacgggc ccccgttcct ggtcggaacg gaggcctcgc gtgtgggtgg
     7021 tacgtcagga cacgccgtcg cagcctgcaa ttgcgtctcg caatgcagcg gcctcgtcgg
     7081 gggtcatttc gacgaccagc cgtccgccac cctccaaagg catgcgcaga acgatcccgc
     7141 ggccttcttt ggtgacctcg agtggaccat ctccggtcct cggcttcatc gccgccatgg
     7201 gttccccttc ctcggaaact cgtgcgccac agcctgatgc gtcggcagat tcacgacgca
     7261 gactgcaccg gagaccatta tcacgcgtct accgggctcg gacgaaatcg tcgtgcggca
     7321 cgatggggcc atgaccgacg atcgtgccgc gcagaccgcg cccgtcctgc tccatgtcga
     7381 gggcggggtc gccactgtga ccctcaaccg tcccgaggcg atgaactccc tcgacctcgc
     7441 gacgaaggac ctcttggtgc cgacgattcg ccaggttgcg gacgatccgg cggtgcgcgt
     7501 cgtcgtcatc accggaagcg gtcgggcatt ctgcgtcggg caggacctca aggagcacat
     7561 tgccgggctg atggccggcg acgaggggct cggggtcacc gtcgtcaagc actacaaccc
     7621 gatcgccgag atgctcgcca cgatgaacaa gccggtcatc gccgccctca atggcgtcgc
     7681 ggccggcgcg ggtgcctcga tcgccatggc tgccgatttc cggatcatgg tcgagggcgg
     7741 cggcatgaac ctcgcgttcg ccgggatcgc gctctcctgc gacaccggtt cgtcgttctg
     7801 gctgccgcgc ctcgtcggtg tcgccaaggc caaggagctg ctcctgctcc cgcggaccct
     7861 ctcggcgcag gagtgcctgg acctcggcct ggtcaccagc gtcgtcccgg ccgaagcctt
     7921 cgccggcgag gtcgccacgc tggccggcaa actcgccgcc gggccgaccc tggcctacgg
     7981 cgcgatgcgt caggcgatcg ccttcagcgc ggcccactcc ctcaccgagt cgttggcgtt
     8041 cgagagtgat ctgatgaact ccacgggcca gaccgccgac catcgggtcg ctgtcgatgc
     8101 cttcctggcc aagcagaagc cggtgttcga gggccggtaa ccggtcgggc cgttagggcg
     8161 cgaagtcgat cggtggcgtg aacagccaca cgaccgagac ccacttccac tgcaaccaca
     8221 ggaaggccgc tccgaggacg agcgcccgga ccgttgtcgg tatgccgcgc gcccgacccc
     8281 gctcgctggc cagccccagc aggaccgcga ggtaggggaa catcggcagc aggtagcgcg
     8341 ccgtgctcgt cccaggggtc tgcaccgcga tgacgtatgc cgcgtagatc accggccaga
     8401 cccgcagctc cggccccagt cgacgagccc acgggccggc catgccgacg agcaggagca
     8461 cgagggtcac ggcgaacagc cgcggccccc agtcgccgag gtagtaccgc gggatcgtga
     8521 accacggctc gaggatctgg acgtgcccgg tcatcgacca ggcgcccatg gtgtcgaagt
     8581 acgccgaggc ctggccggtg gccacccagg cgatccctgg ccacaacagg ccggccactc
     8641 cgcacccgac gaggctggtc agcgccgctc cggtctctcc ggggctcatg ggctctgcgg
     8701 ctcgccgccg ccagcgcagc cacaccgcga caagcgtgac gacccccaag ggcagggcga
     8761 tcggccgggt gaccccgatc aacagagcca atcccgctgc aacgagccag cgttcccggc
     8821 tcagggcgag gaggtatccg cacaacagca gcatggccaa cgactcggtg taggccacct
     8881 gcaacgccgg agaggagggg aagacggcat acaggccgac gacgagcagc gcgcccgcgc
     8941 gaccgatccg ggggcgtagg agggccgcca tggccaccgc cgcgccgaac ccgatgacca
     9001 aggccagggt cgaggcggcg gccgcgaacg agaggccagt cacctccatc acggcgcggg
     9061 cgaggaacgg gaacagcggg tagaaggccc aggtgttctg catggcgatc ccggcagggt
     9121 cccggggcac cgtgttggga tatccgcccg tcgccacccg ctcgtaccac tggccgtccc
     9181 agagtcgagt gaaaccgaca tagtcgacgg cctggtggtc ggtcatgtcg gccggcagtt
     9241 gggacggcgc gacgagggcc agcatgacgg tcgtgagcag gcgcgtgatc gcgtagagga
     9301 cgacgacctg aaccacggcg ttgccgcgga agacccgatc gagcaccgcc tcggcgcgag
     9361 cgaagggtgg cccgctcaac gcggctttgg agtcgggtgc agagtcggct gcggctgcgg
     9421 ctgcggctac ggatgcgggg ctggcgtcga gcggccgtcc gacactcctc cgcccgcgtg
     9481 cgacccccat tcgggtcatg ctaacggggc tcgggggcag ccgggcgagc ctgttggccg
     9541 ccagcccgat ggcacccggc caggtggtcg tcgacgagcc cgcaggcctg catcgcggca
     9601 tacatggtcg tcgggccgag gaagacgaaa ccacgcgcct tgagcgcttt ggtcagtgcg
     9661 accgactccg gcgaggtggc ccgcacatca cccatcccct gcgggcgatc atggtgggcg
     9721 ggggcgtggg cccagacgag cgcatcgagg ccaccgtcat cgcgcaggtc cacgaccgcc
     9781 tgggcattgt ggatcgccgc gtccaccttg cggacgttgc ggacgatccc ggtgtcggcg
     9841 agcagccggg ctcgatcgcc ggcgtcgaag gcggccacca catcggggtc aaagccagcg
     9901 aaggcggccc ggaagccctc gcgcttgcgc aggatggtga gccacgacaa cccggactgg
     9961 aacgcctcga gggtgagccg ctcgaacaac tcacgctcgc cgtgcagcgg aacgccccac
    10021 tcgtcgtcgt ggtaggcgac gtagtcggcg gtggacgcag cccacgcaca gcggggtatg
    10081 ccgtccggcc ccgtgacgac ggcggtcgcg ctcatgcgcg ggcggccatc tcggccactg
    10141 cgcgggtgaa aatcgagcgg acggcccgat cggtgagagg tgcggccagc cgaccgatgg
    10201 catgcgggtg cggcacgatc tcctcacgcc aggtcagtcg cgtccgggtg ccgtcgagcg
    10261 cctcgagccg aatggtggcc caacccccca gccaacggcc ggtcttgacg atcgcgaact
    10321 cggcccgccc gtccgccggc ggcgaccaac gggtgacgac catccggtcg gcgaagcgca
    10381 gccggccgat gccggtcagt gcgacgaacg cccaccccac ccgcggtggt cccgcgtcgg
    10441 tgtcgatccg cgtcaacgtc accggatgcg cttccaggtc gctgacgacc gcccaaacgg
    10501 cttcgatcgg gcgcgggatg actcgctgga tggtgactgc ggtcatggat caggcctgcg
    10561 agtcggggcg ggcgttgggt tcggagaact ccgcccattc ggaggggcgc acgaacggcg
    10621 aggcgggcgg catactcggg tcggcgtcgg ggccaggctg gtaggcggcc gtggtcgcgg
    10681 cgtcgatctg atcgcgcagg acggtgatct cggcggtgag ccggtcgatg acctcgtcga
    10741 cctgctccat ccggtagccc cgaaggcccg tgtccagccg caggccctgg acgtcgtcgg
    10801 gagacagcgg accgtccggg agcggggcgt aggacaacga cgacgtcgga tcgacgagcg
    10861 acccgtcgac ccggccgaag attgcagcga ccgtcaaccc gacgaggacg acggctacga
    10921 ccagcgcaat ccacatcagc gcacctccgt gtggaggtga tcctgatcga cgacgaagcg
    10981 cacggcctgc tcgacgtcgt cggtgacgag gatgcgatcg acgtcgtctg gcgagatggt
    11041 gcccgcggcg acgagggtcg tgcggaacca gtcgatgagc cccgcccagt agtcgcgccc
    11101 caagaggacg atggggaagg ttgtgatctt gccggtctgc acgagggtca gcgcctcgaa
    11161 gagctcatcg agggttccca taccgccggg caggacgatg aagccttggg cgtatttgac
    11221 gaacatcgtc ttgcgggcga agaagtagcg gaagttgacg ccgaggtcga cgtaatcgtt
    11281 gaggccggtc tcgaacggca actcgatgcc cagcccgacg gaggtgccgc cggcttcgag
    11341 ggcgcccttg ttggccgctt ccatgaggcc ggggccgccg ccggtgatga cggcataccc
    11401 ggatgcgacc agtctgcggc ccacctggac cccgagctcg tatgccgggt ggccggctcc
    11461 cagccgcgcc gacccgaaga cgctgacggc cgggccgagc tccgcgagcg ccccgaaccc
    11521 ttcgacgaac tcggactgga tgcgcatgac ccgccacggg
//
LOCUS       DAOMZY010000066        11527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5248_length_11527_cov_5.827232, whole genome shotgun sequence.
ACCESSION   DAOMZY010000066 DAOMZY010000000
VERSION     DAOMZY010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 11527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11527
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5248_length_11527_cov_5.827232"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..829
                     /locus_tag="PLK64_05440"
     CDS             38..829
                     /locus_tag="PLK64_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HPV79217.1"
                     /translation="MTTTVATPTLPPVGIVALAAVAGLQGLLDRFAEGVGAGVDVRYA
                     ASSVTEVDRAIRRLQAIKLKLVAAADRAEVSRGSGHSGTGAWLAAQTHSSGSAAAAQV
                     ALAAALHGAVPGGAGPSEGLSLTSAALSAGQLSPEHAAVIASTSRQLPASLDVAERLR
                     VEAALVEQAQRVDPARLRRTARRALTFAGRADAEVAAHEDAVLRTEESRAFERARLTL
                     HEPRRDDERAFHGAGLRRSDPAQDHPAVGVSASVARESTGRGALG"
     gene            774..1367
                     /locus_tag="PLK64_05445"
     CDS             774..1367
                     /locus_tag="PLK64_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HPV79218.1"
                     /translation="MASPRRWRESRPAAARSDDLAIDWAQRYGQAFTELIEHLPTDRL
                     TGKVAATVVVTLDAQRLADLVNSQPQASGVDTGLTLSAGQARRIACNAGLLPAVLGGP
                     SLPLDLGRADRFFTEAQRVALATIYDECAADGCDRPYAWSELHHEDPWHAGGRTDLAQ
                     AVPLCGWHHRRIHDPAYRHSVTTAPTGAKSVTFHLRT"
     gene            1753..2874
                     /gene="infC"
                     /locus_tag="PLK64_05450"
     CDS             1753..2874
                     /gene="infC"
                     /locus_tag="PLK64_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPV79219.1"
                     /translation="MKEHHISEPRINERIRVPEVRLVGPNGEQVGIVRVEDALRLAAE
                     ADLDLVEVAPMARPPVAKLMDFGKYKYEAALKAREARKNQVNTVIKEIKLRPKIDPHD
                     YGTKKGHVERFLKGGDKVKVTIMFRGREQSRPELGFRLLQKLAEDISELGFVESAPKQ
                     DGRNMIMVIGPTKKKAEAKAEARRARESHSPSGQARSAGSGHAAGDASADEHFDLAEN
                     PSDDSLSLEAQVELAEMAEAAEAIEAAEAAEAAAAHAVVPDAAAATADDIAESVDDAV
                     DEIAPEPAEAPAAPAPVKRAARAVPAPAPAAAATPAAARAAAAPATPGSATPKPAVPR
                     PSAPRPAAPRPTATKAAAPRSSTARPTPAPRPGPRPTSG"
     gene            2953..3147
                     /gene="rpmI"
                     /locus_tag="PLK64_05455"
     CDS             2953..3147
                     /gene="rpmI"
                     /locus_tag="PLK64_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPV79220.1"
                     /translation="MPKMKTHSGAKKRFRVTGTGKIMREQAGGRHRLEVKSSKEMRSL
                     AGDLELSPPDAKKAKRLLGR"
     gene            3179..3565
                     /gene="rplT"
                     /locus_tag="PLK64_05460"
     CDS             3179..3565
                     /gene="rplT"
                     /locus_tag="PLK64_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPV79221.1"
                     /translation="MARVKRAVNAQKKRRVVLERASGYRGQRSRLYRKAKEQMLHSLG
                     YAYRDRRAKKGDFRRLWIQRINAAARANGMTYNRFMQGLKAAEVEVDRKILADLAVND
                     AAAFTALVELARKAVPAQGTSAGDAA"
     gene            3626..4423
                     /locus_tag="PLK64_05465"
     CDS             3626..4423
                     /locus_tag="PLK64_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPV79222.1"
                     /translation="MLTNPRADRVRSVAALSRRSARERAGLFVAEGPQSVREAVRFRP
                     EAIRDLYVTAEAGERHTEIVDAATAAGVHVHECTPEVLAAMSDTDAPQGLLAVCRVLT
                     STLADVLARRPRLLVVLAHVRDPGNAGTVLRGADAAGADAVIVSEASVDVFSPKVVRS
                     TAGSLFHVPVVTGLPVPQILAELAGAGITRLAADGAGTRLLPEVDLTRPHAWVMGNEA
                     WGLPQPLREACDEVVRVPIFGHAESLNLAMAATVCLYASALAQHPAG"
     gene            4519..5673
                     /locus_tag="PLK64_05470"
     CDS             4519..5673
                     /locus_tag="PLK64_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPV79223.1"
                     /translation="MNVADGVALAGVHQTPADVVRAISSTIPDGLLVLDGSRRLVFVN
                     RRAEEILQSPISGSLGLPIEEAVPLWDSDGRLWWDVANPWRQLHTVKGHREKLLWTGS
                     GVEVLATARYLRPEPRQPVERVVVALRDALARQRAERDHAALISTLAHELRSPLTSVK
                     GFSSTLLRRWDRFTDEQKRLIIETIEADADRVTRLVSDLLDVSRIEAGRMQVRRREIF
                     LDPLIARHLRRLTGLGIPESRIEVEVNRPIPDCWADPDRIDQILINLVDNALTHGAGQ
                     IRVRVTSGTVDMVDGADGDARPACIVSVADEGQGIPERLRAVIFTRFWHGPGQRNTGL
                     GLYIVKAMVEAHGGRVRATNGPAGGAVFTFSIPAGDPALALSEAAALSVG"
     assembly_gap    5838..5937
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5938..6990
                     /gene="pheS"
                     /locus_tag="PLK64_05475"
     CDS             <5938..6990
                     /gene="pheS"
                     /locus_tag="PLK64_05475"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPV79224.1"
                     /translation="PAPLEPAALDAALDAAREAFAAAGDLDALKAARLAHVDKGAIAA
                     ARREIGALPPAAKAEAGKLVGAALDAVRAAYTSREEALQAVRDERILVEERVDVTMPT
                     ARRPLGARHPIELTQERLADIFIGMGWEVAEGPELESEWMNFDALNLGKDHPARQMQD
                     TFFVDPPDGGLVLRTQTSPIQIRSMLTRKPPIYVVAPGKVFRTDDLDATHTPVFHQIE
                     LLAVDKGLTMAHLRGAIDHFVRAMFGDVKTRVRASYFPFTEPSMETDFQCFVCRGADD
                     ACRLCGGTGWIELGGSGMVNRKVFAACGIDPDVYTGFAFGFGIERALQLRNGVSDMRD
                     MVEGDVRFSGQFGMAV"
     gene            6992..>8193
                     /locus_tag="PLK64_05480"
     CDS             6992..>8193
                     /locus_tag="PLK64_05480"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPV79225.1"
                     /translation="MRVPLSWLGELVDLAPGARGAEVAASFVSVGLEEEALHGGDISG
                     PLVVGRVLTFDEQIQKNGKPIRFCTVDVGNHGQRVTDGIPQEIVCGATNFEVGDLVVV
                     VLPGAVLPGGFAISARKTYGRVSNGMICAEDELGLGEDHSGIIVLTDYLGESVAAGLT
                     PGDDALALLGLGEQVLELNITPDRGYCFSMRGIAREYWHSQGSPAGAFRDPGVVPTNP
                     PANLDGYAVHLTDAAPIDGAPGCDRYVARIVRGIDPSASSPAWMRTRLTQAGMRPISL
                     AVDVTNYVMLLVGQPLHAFDLDALSGSITVRRARVGERLTTLDDVERECSTEDLLITD
                     GGQVPLALAGVMGGATSEVTATTTNILIEAAHFDPVTIARTSRRHRLVSEASKRFERG
                     VDPRVADDA"
     assembly_gap    8194..8293
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8294..9474
                     /locus_tag="PLK64_05485"
     CDS             <8294..9474
                     /locus_tag="PLK64_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPV79226.1"
                     /translation="GCTVEDPGVAPTEVVFVTPPSWRPDLSDGPDYAEEVARVDGYDR
                     IPSVLPTPSDGRGLTHAQVVRRIVANALVGRGFTEVLSYPFVAADFADVLGMTPDDPR
                     RNAVRLANPLSDQAPLMRTSVLDTLLDSLRRNVSRGQRDVALFELGMVTRPLPGSSNT
                     APVPGVLERPDDETIASIDAAVPPQPRHVAVVLSGDADRAGWWGPGRAADWTDAIDAV
                     RAVAGALSVPVEVTAADLAPWHPGRCAKVTLADGTLLGYAGELHPKALSRLGLPARTC
                     AAEISVEVLTAASEEPTRLRAFSTQPVALTDVALTVAESVAAGELQAVLREAAGEFLE
                     SVILFDVYRGEQVGADKKSMAFRLAFRAPDRTLTTDEVSRFRDAAVAAAAARFGAVQR
                     "
     gene            complement(9487..10455)
                     /locus_tag="PLK64_05490"
     CDS             complement(9487..10455)
                     /locus_tag="PLK64_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPV79227.1"
                     /translation="MTGPVSLTPPTFDDIQRAADAIHHRLPVTPAWSYPALDALAGCE
                     VVVKHENTQPTGAFKVRGGLALAATLTPAHLAAGLVTASTGNHAQSIAYAARLHGATA
                     TIVVPESAPEAKVAAVRLLGANVVVHGPTMTEAVAMAQELAGRDGMTYVDPGNTPAIV
                     AGHATVYLELLTQRPDLEAIYVPIGSGSGAAGACIVRDRLAPDCRIIGVQSSAAPAAY
                     LSWRAGQIETAPCLTRHSGLATGRGFELPQSVIGAGLADFIIVDDAEISDAARVLAHL
                     AHTLAEGAGAAALAGLLKATSHPDRVAVICTGGNASADEIAHLARG"
     gene            10541..>11527
                     /locus_tag="PLK64_05495"
     CDS             10541..>11527
                     /locus_tag="PLK64_05495"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV79228.1"
                     /translation="MTRRRGIPPSWVVLAAGVATALHLGKLPPAVPVLQAELGMTLVQ
                     AGFMLALIQLAGMFLGLAFGIGADGIGLKRSMVIGLAILSVAGVASGFSHHVGGLLVL
                     RAVEGFGFLLATVPAPGLLRRIVPPDQTTRVLGVWGAYVPFGTALGMLVGPRVLAAFT
                     WPIWWWLVAAGTAVMAAAVWVRVPADPPRSASGSGSSSAVAVLSVPWRERASLTVRSP
                     GPWLGALTFGVYGAQWMAVIGFLPTLYAASGWTGALGATLTASVAAVNVIGNISAGVL
                     LHHGWRAEVVVGVGFAGQVIGAFLAFATLTESQPVVRFAGALIFSTVGGLVPG"
BASE COUNT         1588 a         3986 c         4006 g         1747 t
ORIGIN      
        1 cttgccatag gacgtgtgtt cgactagaat cggacctatg accacgaccg tcgccacccc
       61 gaccctgccg cccgtcggca tcgtcgcgtt ggccgcggtt gctggtctgc aggggctgtt
      121 ggatcggttc gccgaggggg tcggggccgg ggtcgacgtc aggtatgccg cgtccagcgt
      181 caccgaagtc gaccgcgcca tccggcgcct gcaggccatc aagctcaagc tggttgccgc
      241 cgccgatcgg gccgaggtgt cgcggggctc cggtcactcg ggcaccggcg cctggttggc
      301 ggcccagacg cattcgtcgg ggagcgctgc cgctgctcag gtggcgctgg ccgcggccct
      361 gcatggggct gtcccaggcg gggccggccc tagcgaggga ctgtccctga cgagtgctgc
      421 gttgtcggct gggcagctct cacctgagca cgccgcggtg atcgcctcga cgagccgtca
      481 gttgcccgcg tcgctcgacg tggcggagcg cctgcgggtg gaggcagctc ttgtcgagca
      541 ggcacagcgg gtcgatcccg cgcggttgcg ccgcacggct cggcgagcct tgaccttcgc
      601 cggtcgggcc gacgcggagg ttgccgcgca tgaggatgcg gtcctgcgga ccgaggagtc
      661 tcgagctttc gagcgagcgc ggctcaccct tcatgaacct cgacgggacg atgagcgggc
      721 atttcacggt gccggccttc gccggtcaga tcctgcgcaa gaccatccag cagttggcgt
      781 ctccgcgtcg gtggcgcgag agtcgaccgg ccgcggcgcg ctcggatgac ctcgcgatcg
      841 actgggccca gcgttacggc caggcgttca ccgagctcat tgagcacctt ccgaccgacc
      901 ggctgaccgg caaggtcgcg gcgaccgtgg tcgtcacgct cgatgcccag cgactggccg
      961 atctggtcaa ctcccagccg caggccagcg gcgtcgacac cggcctcacc ctctccgctg
     1021 gccaggctcg tcggatcgcg tgcaacgccg gcttgctccc cgcagtgctg ggcggcccgt
     1081 cgcttccgct ggacctgggc cgagctgatc ggttcttcac cgaagcccaa cgcgtcgctc
     1141 tggccaccat ctatgacgag tgcgccgccg atggatgcga ccggccttac gcctggagcg
     1201 agttgcacca cgaagatccg tggcatgcag gtggccgaac cgacctggcc caggcggtgc
     1261 cgctgtgcgg ctggcaccat cggcgcatcc acgacccggc ctaccggcac agcgtgacca
     1321 ctgccccgac cggcgcgaag tcagtgacat tccacctgag gacctgaggc gaagagccgt
     1381 tgacggtgcg gcgcgaaccg gcgcggacac ggcatacccg cgccgtcccc gaatggactg
     1441 gctaggcgag tggcccttac ctggtaatct agggaactga ccagccgcgt ccgacgcacc
     1501 ccttccaggg agcagagggc gcggtgtgca agagaagccc cgcttccacc cgcgccgatc
     1561 accagtcgcc gggtcaggtc cggacccgcc cggctcgccg ggagcgtcgg tgtatgccgt
     1621 cgcgctgggt cccgcgcgtg tgcgcggcag gcgactgccc ggcatccgca gcggacggac
     1681 aagcgaaggc tcctcgtgca ccgacggtga cgaagggcct tctttcgttt cccggggtca
     1741 ctcgtgaccg acatgaagga gcaccacatc agcgagcctc gtatcaacga gcgcatccgg
     1801 gtccccgagg tgcgcctggt cggacccaat ggggagcaag ttggcatcgt ccgcgtcgag
     1861 gacgccctgc gcctggctgc cgaggccgat ctcgacctcg tcgaagtcgc gcccatggcg
     1921 cgtcccccgg tggccaagct catggacttc ggtaagtaca agtacgaagc cgccctcaag
     1981 gccagggaag cgcgcaagaa ccaggtcaac acggtcatca aggagatcaa gctccgaccg
     2041 aagatcgacc cgcatgacta cggcaccaag aagggccacg tcgagcgctt cctcaagggc
     2101 ggcgacaagg tcaaggtgac gatcatgttc cgaggtcgcg aacagtcgcg cccggagctg
     2161 ggtttccggc tgctgcagaa gctcgccgag gacatttcgg agctcggttt cgtcgagtcc
     2221 gcgcccaagc aggacgggcg caacatgatc atggtcatcg gcccgaccaa gaagaaggcc
     2281 gaggccaagg ctgaagcccg tcgggcgcgc gagtcgcact cgccgtcggg tcaggctcga
     2341 tcggccgggt cggggcacgc tgccggggac gcatcggcgg acgagcactt cgacctcgcc
     2401 gagaacccgt cggacgactc gctgtccctg gaagcccagg tcgagttggc cgagatggcc
     2461 gaagcggcag aggcgatcga ggctgcggaa gcggccgagg cagcagccgc gcacgctgtg
     2521 gtgccggacg ctgcggcggc caccgctgat gacatcgccg agtcggtcga cgacgcggtc
     2581 gacgagatcg ctcccgagcc ggccgaggcg ccggccgcgc cggctccggt caagcgggca
     2641 gcccgcgcgg ttcccgcccc ggcgcctgcc gccgcggcca ccccggcagc ggcccgtgca
     2701 gcggcagccc ctgccacccc cggctctgca actcccaagc cggccgtccc ccgccccagc
     2761 gctccgcgcc cggcggctcc gcggccgact gccaccaagg cggccgcccc acgatcgagc
     2821 acggcccggc ccacgccggc gccgcgaccg gggccccgtc ccacgtcggg ctgacccagc
     2881 ccgcacccag accacatctg accccgtccg accgacgtcg agcgggacct gacacgaagg
     2941 agatcggcag ccatgccgaa gatgaagacc cacagcgggg ccaagaagcg cttccgcgtg
     3001 accggcaccg gcaagatcat gcgcgagcag gccggcggcc gtcaccgcct cgaggtgaag
     3061 tcgagcaagg agatgcgctc gctcgccggt gacctcgagc tgtccccccc cgacgccaag
     3121 aaggccaagc ggcttctcgg ccgctgagcc cgacccactc acgtaaagga gaactcacgt
     3181 ggcacgcgtg aagcgggcag tcaacgccca gaagaagcgc cgggtcgtcc tcgagcgcgc
     3241 cagcggctac cgcgggcagc gctcgcggct ctaccgcaag gccaaggagc agatgctcca
     3301 ctcgctcggt tacgcctacc gcgaccgccg ggccaagaag ggcgacttcc gtcgcctgtg
     3361 gatccagcgg atcaacgccg cggcccgcgc caacggcatg acctacaacc ggttcatgca
     3421 gggtctcaag gccgccgagg tcgaggtcga ccgcaagatc ctcgccgacc tcgccgtcaa
     3481 cgacgccgcg gccttcaccg cgctggtcga gttggcccgc aaggcggtgc ccgcgcaggg
     3541 cacgtccgcc ggcgacgcag cctgagccac acggcatacc gctgcacgac actcaccgca
     3601 tgacgctgag cgccgggaca cccgcgtgct gaccaatccc cgagccgatc gggtgcggtc
     3661 ggtcgcggcg ctgtcccggc gctcagcgcg tgagcgggcg gggttgttcg tcgccgaggg
     3721 gccgcagtcg gtccgcgagg cggtccggtt ccggcccgag gcgatccgcg acctctacgt
     3781 cacggcggag gcgggggagc ggcataccga gatcgtcgac gcggccaccg cagcgggcgt
     3841 ccacgtgcac gagtgcaccc ccgaggtcct cgccgccatg tccgacaccg acgcgccgca
     3901 ggggctgctc gcggtatgcc gtgtgctcac ctccaccctc gcggacgtcc tcgcgcgccg
     3961 tccccggctg ctcgtcgtcc tggcccacgt ccgcgatccc ggcaacgcgg ggaccgtgct
     4021 gcgcggagcc gatgctgcgg gtgccgacgc tgtcatcgtc agcgaggcga gcgtcgacgt
     4081 cttcagcccc aaggtggtcc ggtcgaccgc cgggtcgctc tttcacgtgc ccgtggtcac
     4141 cggcctgccg gtcccgcaga tcctcgccga gttggccggg gcggggatca cccggctggc
     4201 cgccgacgga gccggaaccc gcctcctgcc cgaggtcgac ctgacccggc cccacgcctg
     4261 ggtgatgggg aacgaggcgt gggggctgcc gcagccgctg cgcgaggcct gcgacgaggt
     4321 cgtccgggtg ccgatcttcg gtcatgccga gtcgctcaat ttggcgatgg cggccaccgt
     4381 ctgcctctat gcctcggcgt tagcacaaca tccagcaggg tgacccgcgg ggctgcgcgt
     4441 ccgcgccatg gatcacatca cggatcaatc ttgagcagga tcgtcgcggt tgactgcggc
     4501 ggtgactaat ctggcgacat gaacgtcgcg gatggggtgg cgcttgcggg cgttcaccaa
     4561 actcccgcag acgtggttcg ggccatctcg tcgacgattc ccgatggcct cctggtgctg
     4621 gacggttcgc gtcggctcgt gttcgtcaac cgccgggccg aggagatcct ccagtcgccg
     4681 atcagcgggt ccttgggcct gcccatcgag gaggccgtgc cgctctggga ctccgatggg
     4741 cggctgtggt gggacgtcgc caatccctgg cgacagttgc acaccgtcaa gggccatcgg
     4801 gagaagttgc tgtggaccgg ctcaggggtc gaggtgctcg ccacggcacg ttacctgcgg
     4861 cccgagccgc gccaaccggt cgaacgagtc gtcgtcgcct tgcgcgatgc cctcgcccgc
     4921 cagcgcgccg agcgcgacca cgcagcgctc atctccaccc tcgcccacga gttgcgctca
     4981 ccgctcacct cggtcaaggg cttctcctcg acgctgttgc gtcgttggga ccgattcacc
     5041 gatgagcaga aacggctcat catcgagacc atcgaggctg atgccgaccg ggtgacccgg
     5101 ctggtgtctg atctgttgga tgtctcgcgg atcgaagcgg gccggatgca ggtgcggcga
     5161 cgggagatct tcctggatcc cctcatcgcc cggcatctgc gcaggctcac gggcctcggc
     5221 atcccggaga gccgcatcga ggtcgaggtc aaccgcccca tcccggactg ctgggccgat
     5281 cccgaccgga tcgaccagat cttgatcaac ctcgtcgaca acgccctgac ccacggcgcc
     5341 gggcagatcc gggtgcgggt gaccagcggg acggttgaca tggtcgacgg cgccgacggc
     5401 gacgctcgcc ctgcgtgcat cgtctccgtc gccgatgagg gacaaggcat cccggagcga
     5461 ctccgagcgg tcatcttcac ccggttctgg cacgggccgg gccaacgcaa caccggcctg
     5521 gggctctata tcgtcaaggc gatggtcgag gctcacggcg ggcgggtccg agccaccaat
     5581 ggcccggctg gaggggcagt cttcacgttc agcatccccg ccggtgaccc cgccctcgcg
     5641 ttgtccgagg ctgcggcact gagcgtcggc tgagcggttc ccgccccgtg ggacggtcca
     5701 ggccatgaac tcgatgtcga ggccaccctc gcgcggccta gactcgggcg ccgaacccga
     5761 acgccgcgac cagcccgaat gtggaacgtg atgtcaggac caactgctcc gctcgaccct
     5821 gctccgctcg agcctgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncct
     5941 gctccgctcg agcctgccgc gcttgacgcg gccctcgatg cagcccgcga ggcgttcgcc
     6001 gctgctggcg acctcgatgc tctcaaggcc gcccggctcg ctcacgtcga caagggcgcc
     6061 atcgccgcgg cccgccgcga gatcggtgcc cttccgccgg ccgccaaggc ggaggccggc
     6121 aagttggtgg gcgccgcgct cgacgccgtg cgcgcggcat acacctcgcg cgaggaggcc
     6181 ctgcaggcgg tgcgcgacga gcggatcctc gtggaggagc gcgtcgacgt caccatgccg
     6241 accgcgcggc ggccgttggg tgcgcgccac ccgatcgagc tcacccagga gcggctggcc
     6301 gacatcttca tcggcatggg ctgggaggtc gccgaaggcc ccgagctgga gtcggagtgg
     6361 atgaacttcg acgcgctcaa cctcggcaag gaccacccgg cccgccagat gcaggacacc
     6421 ttcttcgtcg acccgcccga cggcgggctg gtgctgcgca cccaaacctc gccgatccag
     6481 atccgcagca tgctcacccg caagcccccg atctatgtcg tcgcgccggg caaggtgttc
     6541 cgcaccgacg acctcgacgc cacgcacacg ccggtcttcc accagatcga gttgctggcg
     6601 gtcgacaagg gcctgacgat ggcgcacctg cgcggggcca tcgaccactt cgtccgggcc
     6661 atgttcggcg acgtcaagac gcgcgtgcgc gcctcctact tcccgttcac cgaaccttcc
     6721 atggagaccg acttccagtg cttcgtatgc cgtggggccg acgacgcgtg tcgcctctgc
     6781 ggagggacgg gctggatcga gctcggcggg tcggggatgg tcaaccgcaa ggtgttcgcg
     6841 gcgtgcggga tcgaccccga tgtctatacg ggcttcgcgt tcggcttcgg catcgagcgc
     6901 gccctgcaac tgcgcaacgg cgtgagcgac atgcgggaca tggtcgaggg cgacgtgcga
     6961 ttctccggtc agttcgggat ggcggtgtga catgagggtt cccctgtcct ggctcggcga
     7021 gctcgtcgat ctcgctcccg gtgctcgcgg cgccgaggtc gccgcgtcgt tcgtctcggt
     7081 cggtctcgag gaggaggcgc tgcacggcgg cgacatctcg ggcccgttgg tggtcgggcg
     7141 cgtcctgacc ttcgacgagc agatccagaa gaacggcaag ccgatccggt tctgcacggt
     7201 cgatgtgggc aaccacgggc agcgcgtcac cgatggcatc ccgcaggaga tcgtttgcgg
     7261 cgcaacgaac ttcgaggtcg gtgacctcgt cgtggtggtg ctgcccggcg ccgtcctgcc
     7321 tggcgggttc gccatctcgg cgcgcaagac ctatggtcgg gtctccaacg ggatgatctg
     7381 cgccgaggat gagttgggcc tcggcgagga ccactcgggg atcatcgtgc tgaccgacta
     7441 cctcggtgag tcggtggcgg caggcctgac gccgggggat gacgctctgg cgctcctcgg
     7501 cctgggtgag caggtcctcg agctcaacat cactcccgac cggggctact gcttctcgat
     7561 gcgcgggatc gcccgcgaat actggcactc gcagggcagc cccgcgggcg ccttccgcga
     7621 cccgggtgtc gtccccacca acccgccggc caacctcgac gggtatgccg tgcacctcac
     7681 cgacgcagcc cccatcgacg gcgcacccgg ctgtgaccgc tacgtcgccc ggatcgtgcg
     7741 gggcatcgac ccgtcagcgt cctcaccggc gtggatgcgg acccggctca cccaggccgg
     7801 gatgcggccg atctcgttgg cggtcgacgt caccaactac gtcatgctcc tcgtcgggca
     7861 gcccctgcat gccttcgacc tggacgccct gtcggggtcg atcaccgtcc gccgcgcgcg
     7921 ggtgggggag cggctcacca ccctcgatga cgtcgagcgg gagtgctcga cggaagacct
     7981 gctcatcacc gatggcggtc aggtcccgct ggctctggcc ggcgtcatgg gcggggccac
     8041 cagcgaggtt acggcgacga cgacgaacat cctcatcgag gccgcccact tcgacccggt
     8101 gacgatcgcg cgcacctcgc gtcggcaccg gctggtcagc gaggcctcca agcggttcga
     8161 gcggggcgtc gatccgcggg tcgctgacga cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnntgggctg caccgtcgag gacccggggg tggcccccac ggaggtcgtc
     8341 ttcgtcacgc ccccgtcgtg gcggccggac ctcagcgatg gcccggacta cgccgaggag
     8401 gttgctcgcg tcgacggcta cgaccggatc ccgtcggttt tgccgactcc gagcgacggc
     8461 cgcgggctga cgcacgccca ggtggtccgg cggatcgtgg ccaacgcgct ggtcgggcgg
     8521 ggcttcaccg aggtgctcag ctatccgttc gtcgcggcgg acttcgccga cgtgctcggg
     8581 atgactccgg acgacccccg ccgtaacgcc gtccgcctgg ccaacccgtt gtccgaccag
     8641 gcgccgttga tgcgcacctc ggtcttggac acgctgctgg attcgttgcg ccgcaacgtg
     8701 tctcgtgggc aacgtgatgt cgcgctcttc gagctgggca tggtgacgcg gccactgccg
     8761 ggttcatcga acaccgctcc ggtgcctggc gtcctggagc ggcctgacga cgagaccatc
     8821 gcgtccatcg atgcggccgt gccgccgcag ccgcggcatg tcgcggtcgt cctatccggc
     8881 gacgccgatc gagcagggtg gtggggcccc ggccgcgcgg ccgactggac cgacgcgatc
     8941 gacgccgttc gcgccgtggc cggtgcgctc tccgttcctg tcgaggtgac cgcggccgac
     9001 ctcgcgccct ggcaccctgg acgatgcgcg aaggtcaccc tcgccgacgg caccctgctc
     9061 gggtatgccg gtgagctgca ccccaaggcg ctcagccgcc tcgggctgcc ggcccgcacg
     9121 tgtgctgcgg agatctccgt cgaggtgctc accgcagcca gcgaggagcc gacccggctg
     9181 cgcgccttct cgacccagcc ggtggcgttg accgacgtcg cgctcaccgt ggccgagtcg
     9241 gttgccgcgg gcgagttgca ggcggtcttg cgggaggcgg ctggcgagtt cctcgagtcg
     9301 gtgatcctct tcgacgttta tcgcggcgag caggtcgggg ccgacaagaa gtcgatggcc
     9361 ttccgcctgg ccttccgcgc ccctgaccgg accctgacga ccgatgaggt cagccggttc
     9421 cgcgacgcgg cggtcgccgc tgctgcggcc cggttcggag ccgtccagcg ctgacgaccc
     9481 gctgggtcag ccgcgggcga ggtgagcgat ctcgtcggcg ctcgcgttgc cgccggtgca
     9541 gatcacggcc acgcggtcgg ggtggctggt ggctttgagc agcccggcga gtgccgcggc
     9601 gccggcaccc tcggcgaggg tgtgggccag gtgggcgagc acccgggccg cgtcgctgat
     9661 ctcggcgtca tcgacgatga tgaagtcggc cagcccggcc ccgatcaccg attggggcaa
     9721 ttcgaatccc cgccccgtcg ccagcccgct gtggcgggtg aggcacggtg ccgtctcgat
     9781 ctgtccggcc cgccaggaga ggtatgccgc gggcgccgca ctggactgca cgccgatgat
     9841 gcggcagtcc ggggcgagcc ggtcgcggac gatgcaggca cccgcggcgc cgctgccgga
     9901 cccgatggga acatagatcg cctcgaggtc cgggcgctgg gtgagcagct cgagatagac
     9961 ggtggcgtgg ccggcgacga tcgccggggt gttgccggga tcgacgtagg tcatcccgtc
    10021 gcggcccgcc aactcctggg ccatggcgac ggcctcggtc atcgtcggtc cgtggacgac
    10081 gacgttcgcg ccgaggagcc ggacagcggc caccttggct tccggtgcac tctcgggcac
    10141 gacgatggtc gcggtcgcgc cgtgcaggcg cgcggcatac gcgatggatt gagcgtggtt
    10201 tcccgtggag gcagtgacca ggcccgcggc gaggtgcgcg ggggtcaggg tcgcggcgag
    10261 cgccaggccg ccgcgaacct tgaacgcccc ggtgggctgg gtgttctcgt gtttgacgac
    10321 cacctcgcag ccggcgagcg catcgagggc cgggtaggac cacgccgggg tgacgggcag
    10381 ccggtggtgg attgcgtcgg ccgctcgctg gatgtcgtcg aaggtcggag gagtgagact
    10441 cacggggccg gtcacccgat gagtgtggat cgccggtgcc tctgcggtcc aatccgccgc
    10501 tgcccacaac gggtgtcggc ggcccggtag attgccccga gtgacccggc gccgcggcat
    10561 acctccctca tgggtggtcc tcgcggcggg agtcgcgacc gcgttgcacc tgggcaagtt
    10621 gcccccggcg gtgcccgtcc ttcaggccga gctggggatg accctcgtgc aggcgggctt
    10681 catgctcgcc ctcattcagc tcgctgggat gttcctcggg ttggccttcg ggatcggtgc
    10741 cgacgggatc gggctcaagc gcagcatggt catcggcctg gccatcctgt cggtcgcggg
    10801 ggtggccagc ggtttcagtc atcacgtcgg cgggctgctc gtcctgcgcg cggtcgaggg
    10861 cttcgggttc ctgctggcga ccgtccctgc tccagggctg ctgcggcgga tcgtcccgcc
    10921 ggatcagacc acacgggtcc tgggggtttg gggtgcatac gtcccgttcg ggactgccct
    10981 cggcatgctc gtcgggccgc gagtcctggc cgcgttcacg tggccgatct ggtggtggct
    11041 cgtggcggca ggcaccgcgg taatggcagc cgcggtctgg gtacgcgtgc ctgccgaccc
    11101 gcctcggtcc gcgtcggggt ccgggtcgtc gtcggcggta gcggtgctgt cggtgccctg
    11161 gcgcgagcgc gcctcgctca ccgtgcgctc gccgggcccg tggctgggcg cgctcacctt
    11221 cggggtgtac ggcgcccagt ggatggccgt tatcggcttt ctccccacgt tgtatgccgc
    11281 gtccggctgg accggtgcgc tcggggccac gttgacggcg agcgtggcgg cggtcaacgt
    11341 catcggcaac atcagcgctg gcgtgttgtt gcaccacggc tggcgggcgg aggtggtcgt
    11401 cggggtgggc ttcgcaggcc aggtgatcgg cgcgtttctg gcgttcgcca cgctgaccga
    11461 gagccagccg gtggtgcgat tcgccggggc gctgatcttc tcgaccgtcg gcggcctcgt
    11521 cccgggg
//
LOCUS       DAOMZY010000067        11439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5302_length_11439_cov_5.986033, whole genome shotgun sequence.
ACCESSION   DAOMZY010000067 DAOMZY010000000
VERSION     DAOMZY010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 11439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11439
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5302_length_11439_cov_5.986033"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..519)
                     /locus_tag="PLK64_05500"
     CDS             complement(7..519)
                     /locus_tag="PLK64_05500"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPV79229.1"
                     /translation="MSRLRGSGMPPAHVSEDAVRPRLVASDLDGTLLRPDGSVSDRTR
                     RAVWGLADQGIAFVIVTARPPRWLHELADLVGPHGAAVCANGAFVYDVAARTVREVHG
                     IPDGLVTDLVGELRAAVPGVQFGAERASGAMVEPGFPGAGLDRGAEAVQVASIHDRGD
                     EPVGKLHLLN"
     gene            complement(516..1634)
                     /locus_tag="PLK64_05505"
     CDS             complement(516..1634)
                     /locus_tag="PLK64_05505"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HPV79230.1"
                     /translation="MTGQDRPAELHFELTPNDHPRSDEERAALLANPGFGKVFTDHMA
                     LATWTTEAGWHDAQVRAYGPLSLMPAAAVLHYAQEVFEGLKAYRHADGSIWSFRPEAN
                     AQRLQRSCRRLALPELAVDDFVGSLKALVEVDKAWVPAVGTGETSLYLRPFMFASEAF
                     LGVRPAAAVTYAVIASPAGAYFSGGLKPVTLWISQDYARAGAGGTGAAKCGGNYASSL
                     AGQLEGIEHGCDQAVFLDSGTHTYIEELGGMNLFFVTSDGKLVTPELTGTILEGITRS
                     SILELAKELGLEPEERRIPIEEWKDAARSRKITEIFACGTAAVVTPVGELRWEGGSVD
                     HRGDGPAIGEVTMTIRERLLDIQYGRVEDTNGWLTRLA"
     gene            1789..2955
                     /locus_tag="PLK64_05510"
     CDS             1789..2955
                     /locus_tag="PLK64_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPV79231.1"
                     /translation="MNRAVRALRGNHGRYAASAPLAARPLIAGGALGTAGVIAVLLFS
                     ASSHADVSSSAVGSTVAAGRPSATSSTTAGGESGLTKRPESEVPHFNAVNAMTAAAAN
                     AAAAQAAALAELDAAPDAGSVPTAVVDAAQVLASGFVKPVDGVASSPFGVRFHPILHV
                     WKLHSGTDLATSCGTPVKAAKAGQVTLAGGFTGYGNRVVVDHGSGLVTTYNHLRSYAA
                     VAGLKVQQGQIIGYVGSTGMSTGCHLHFEVMVGGAFVDPAPYLDLAPAPKVVIPAAVP
                     ARSDVILAPAPTLTTTPPVTPTLTPTETAVPTETVVPTATASPTETATTSPTDVPTST
                     VGPSPTSDPTSTATDPPSLTSDPTSPTSEPSAPTATPTSDPVQSSPTATDPASP"
     gene            complement(3001..4077)
                     /locus_tag="PLK64_05515"
     CDS             complement(3001..4077)
                     /locus_tag="PLK64_05515"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HPV79232.1"
                     /translation="MVDTSVTTPEPTSLNLAVIGGDGIGPEVVGEGLKVLAAVTGGAV
                     SISTTEYDLGARRWHATGETLPDSVLAELRGHDAILLGAIGDPTVPSGVLERGVLLPI
                     RFALDHFVNLRPAKLYPGVASPLDIARVAPDGIDFVVVREGTEGPYVGNGGLLRAGTP
                     HEVATEVSVNTRFGVERVVRDAFARAQGRPRKHLTLVHKHNVLTFAGHLWRRTVEDVA
                     KEFPEVQTAYQHVDAATIFLATDPGRFDVIVTDNLFGDIITDIAAAIAGGIGLAASGN
                     INPTRSTPSMFEPVHGSAPDIAGQGKADPTAAILSVAMMLDHVGLADAAARVEAAVAA
                     DLVERGNQVRSTVAIGDAIAARLA"
     gene            complement(4163..5746)
                     /gene="serA"
                     /locus_tag="PLK64_05520"
     CDS             complement(4163..5746)
                     /gene="serA"
                     /locus_tag="PLK64_05520"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HPV79233.1"
                     /translation="MTKPVVLIAEELSPATLDALGPDFEIRSCDGADRAVLLPALADV
                     DAVLIRSATKMDAEAISAAKRLKVIARAGVGLDNVDVPAATQAGVMVVNAPTSNITSA
                     AELAVGLLLASARNIAPANQALKAGAWKRSKYGGVELLDKTVGIVGLGRIGALVAERL
                     KGFGMQVLAYDPYTTPAKAGQLGARLVELDELLAQADFITVHLPKTKETLGLIGVDAL
                     SKVKPTVHIVNAARGGIVDEVALADAIREGRVAGAGIDVFVKEPCTDSPLFAFEQVVV
                     TPHLGASTEEAQEKAGIAVAKSVRLALGGEIVPDAVNVSGGVIVEEVRPGIALVEKLG
                     RVFTAVAGAVPLHLDLDVCGEITEHDVSIWKLAALKGLFTDVVEDQVSYVNAPVIAEA
                     RGVGVTLTTDSASGDFRNVTTLRGTLKDGTEVSVAGTLTGPRMIEKLVGINGFDLEVP
                     LSDHLAAFTYADRPGIIGQVGGILGDSAINVGGMQVARSEKRDTALMVLSVDSELPAE
                     VAARIAEGIGAVHFAVVNL"
     gene            complement(5902..7434)
                     /locus_tag="PLK64_05525"
     CDS             complement(5902..7434)
                     /locus_tag="PLK64_05525"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPV79234.1"
                     /translation="MPDITVGAWFAARAARSPQRRALIFEGTSWTFAELADRIERLAA
                     VLRAGGVKHGDRVSFVGFNQPAALETWFACSRLGAIYVPLNFRLAGPELAFIVGDAGV
                     HTLIAGADQRPGIDAIRAELGVRRYLVTEADGIPEGWESYEQAVTAAHPLPTPDPVRP
                     DELSIIMYTSGTTGTPKGVMLSHENLWWSDIALMSWIDIHADDVTLAFSPLFHIAGLN
                     FMIGITWMRGGEVILHRSFDAQRTLEDIARYRVNTLFGVPAMFIAISQLPGFQAADLS
                     TVRMAVCGGAPAPEPLLRTFADRGIGVLQGYGLTETSPAAIFLVAEYALSKLGAAGQP
                     PLYIETKLVGSGGVTVSEPGVRGEVCMRGPNVTRGYWNRPEATAAAFDEAGWFHTGDV
                     GQFDEDGIISIVDRVKDMVISGGENVYPAEVESVLFGHPSVAEVAVIGLPDARWGEAV
                     TAVVAAKPGQSVTLEDLREYAASHLARFKLPQRLHLVEVLPRNPTGKVLKYQLREDLG
                     AK"
     gene            complement(7472..8011)
                     /locus_tag="PLK64_05530"
     CDS             complement(7472..8011)
                     /locus_tag="PLK64_05530"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HPV79235.1"
                     /translation="MLGEPHDRPRPAASDFVDTVADLTQRRAEGADPETTALVVALLR
                     AGGRHRGLFERRAHDQGGRHLRAFSVLYLVWLYGRLRARDLARLLGMSRQTTSAVLAS
                     LESGGLVCRERGEGRDRRLVEVRLTDDGRRTVEREFQAQHDLDGQWLAVLSPAEQRTL
                     RHLLERLATQHPGDNTLVG"
     gene            complement(8025..9638)
                     /locus_tag="PLK64_05535"
     CDS             complement(8025..9638)
                     /locus_tag="PLK64_05535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HPV79236.1"
                     /translation="MQQLTALDVMFSSLDTETTNGVLGGLVLHEPAADGRPAADAAFM
                     RQRFAERLPFLPPLHREVVKVPIGIDHDYLGQPDRIDLLEHIRSISLPAPGTHDQLAA
                     EVSRIMSTSLPTGRPLWDYTVIDGLADGSVAHLLRIHHLVIDGGSMPTLWDALSDEPA
                     APLEAWAESTRPQPRFGKGEMLARELGGVVAKPVQFAAFQAKFLAWVAGQTKEKGPLA
                     PLALPFRVSLPGGLAQPAQALFNSALSKRGIAEIQPYIPTLRTPTTPFNNRVTAQRTF
                     VFADLPLAQFRDTGKALGGTINDVVLGVCAGALRRYLVENGIPTDDPLIVCVPVSIRT
                     GAETTKWANYVHMIFAPLPTHLDDPLARVRAAGAAVKAAKGNFDALPVGLIREASRFI
                     PSAMFNVMTRAMVKMPDKVSKGPWNVVVSNVRGPSTAAYMDGVKIKGYWPASFLSVGG
                     GINITLQSYSDRICFGFMGAPEQVGDLRPLIGYMTDALAETVAAAGDSSAKAAPAEAK
                     AARKRTPHRAGPGSARLAAVAPAAVGDAT"
     gene            complement(9824..10369)
                     /locus_tag="PLK64_05540"
     CDS             complement(9824..10369)
                     /locus_tag="PLK64_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPV79237.1"
                     /translation="MMGSQTPASDVPGAEFRARAVRTTQERRPDVDPRAIALSLTLAR
                     IAATHQHQSERLVHRERGWSYSGFRVLYMIWLTEPVEARDLARFAGVSRQTTSTVLST
                     LEAARLVVRRQTSKTDRRLVSVRLTPRGRAAVDEAIGAQNALESDWFSVLNSAEQAQL
                     QDMLERVLTRIDRPAETPAAD"
     gene            complement(10442..10945)
                     /locus_tag="PLK64_05545"
     CDS             complement(10442..10945)
                     /locus_tag="PLK64_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HPV79238.1"
                     /translation="MFDGLPFPLVYAVFFLGAMIRANATYWLGRGARKGGETTRAARR
                     LHSPAIRRAETVISRFGAPAVALSFLTVGVQTALNFAAGVLRMPLWRYEVGVVIGALM
                     WAGIYSTIGFAVFEAWVSQSPWLWLGAAIGLIALVVGATRLTNRRLESTPPYASFPDV
                     ADSGGRD"
     gene            complement(11073..>11439)
                     /locus_tag="PLK64_05550"
     CDS             complement(11073..>11439)
                     /locus_tag="PLK64_05550"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493182.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HPV79239.1"
                     /translation="EVAYFECLHELKLIVDLMVEGGIAKQRWSVSDTAEYGDYVSGPR
                     VIDPSVKDNMRAVLTDIQSGAFARRFIADQDAGAPEFKALRAKGAQHPIEATGRDLRK
                     LMSWIKETDSDYTEGSAAR"
BASE COUNT         1792 a         3949 c         3984 g         1714 t
ORIGIN      
        1 gatgaattaa tttagcaaat gcagtttgcc caccggctcg tcgccgcgat catgaatcga
       61 ggccacctgg accgcctcgg cccctcggtc gaggcccgct cccggaaatc ccggctccac
      121 catcgccccg ctggcccgct cggccccgaa ctgcaccccg ggaacggccg cgcgcaactc
      181 accgacgagg tcggtgacaa gcccatcggg tatgccgtgc acctcccgga ccgtccgggc
      241 cgcgacgtcg tagacgaaag cgccgttggc acagaccgcc gctccgtgcg gaccaacgag
      301 gtcggccaac tcgtgcagcc agcgcggcgg ccgggcggtg acgatgacga acgcgatgcc
      361 ctggtcggcc agaccccaca cggcccgcct ggtccggtcc gacacgctgc cgtccgggcg
      421 caacagcgtt ccgtccagat cggaggcgac caaacggggt cgcaccgcgt cctcggaaac
      481 gtgagcgggc ggcataccgg atccgcgcag gcggctcacg ccaggcgagt gagccagccg
      541 ttggtgtcct cgacgcggcc gtactggatg tccagcagcc gctcgcggat cgtcatggtc
      601 acctcaccga tcgcggggcc gtcgccgcgg tgatcgaccg agccgccctc ccagcgcaac
      661 tcgcccaccg gcgtgacgac cgctgccgtg ccgcacgcga agatctcggt gatcttcctg
      721 ctgcgggccg cgtccttcca ctcctcgatg gggatgcgcc gctcctcggg ctccaggccc
      781 aactccttag ccaactcgag gatggagcta cgggtgatcc cctcgaggat ggtcccggtg
      841 agctcaggcg tgacgagctt gccgtcggaa gtgacgaaga agaggttcat cccgccgagc
      901 tcttcgatgt aggtgtgcgt cccagagtcg aggaagaccg cctgatcaca cccgtgttcg
      961 atgccctcca actgaccggc caggctcgag gcgtagttgc cgccgcactt ggctgcgccc
     1021 gtgccccccg ctccggcgcg ggcgtagtcc tgcgagatcc agagggtcac aggcttgagg
     1081 ccgccgctga agtaggcacc ggcgggcgag gcgatcacgg catacgtcac cgcagcagcc
     1141 gggcgcacgc cgaggaacgc ctctgaggca aacatgaacg gccgcaggta aaggctggtc
     1201 tcgccggtgc cgaccgcagg cacccacgcc ttgtcgacct cgacgagggc cttgagtgac
     1261 cccacgaagt catcgacagc cagctcgggc agcgccaggc gccggcaact gcgctgcagc
     1321 cgctgggcgt tggcctccgg gcggaagctc cagatcgacc catccgcgtg ccggtaggcc
     1381 ttgagcccct cgaagacctc ctgggcgtag tgcaggaccg cggcggccgg catgagcgag
     1441 agggggccgt aggcgcgcac ctgcgcgtcg tgccagccgg cctcggtcgt ccaggtggcc
     1501 agggccatgt gatcggtgaa taccttgccg aaccccgggt tggccaacag cgcggcgcgc
     1561 tcctcgtcag acctcgggtg gtcgttcgga gtgagctcga agtgcagctc agctgggcgg
     1621 tcctggccgg tcatctgcgt ggctctcctc ggaacgtgtc ggtgggacgg aaacgttatg
     1681 ctgtcagtca gggtacccac tctcccggtt cacctgcgta ggtgaatctc gggccctaag
     1741 gttggtgcag agccaggggg gctcatgccg ttgagggagg gacgaagcgt gaaccgtgcg
     1801 gtccgcgcgc tgcgaggcaa ccatggtcgg tatgccgcat cggcaccact cgctgcgcgc
     1861 ccgctcatcg ccggaggtgc cctcgggacg gccggagtga tcgcggtgct cctcttctca
     1921 gcctcgagcc acgccgacgt gtcatccagc gcggtcggct cgaccgtggc cgctgggcgg
     1981 ccctcggcaa cctccagcac gactgcgggc ggcgagtccg gcctgaccaa gcgcccggaa
     2041 tccgaggtgc cccacttcaa cgcggtcaac gccatgacgg ccgccgccgc gaacgctgcc
     2101 gccgcccagg ccgccgccct ggccgagctc gacgcggcgc ccgacgccgg ctctgtgccg
     2161 accgctgtgg tcgacgcggc acaggtcctc gcctcaggct tcgtcaaacc ggtcgacggc
     2221 gtcgcctcct cgccgttcgg cgtccggttc cacccgatcc tgcacgtctg gaagttgcac
     2281 tcgggcaccg atctggcgac ctcgtgtggc acaccggtca aggccgccaa agccggtcag
     2341 gtgacgctcg ccggcgggtt caccgggtat ggcaaccggg tggtcgttga ccacgggtcc
     2401 ggtctggtca ccacctacaa ccacctgcgc tcgtatgccg cggttgccgg cttgaaggtc
     2461 cagcagggac agatcatcgg ttacgtcggg tcgaccggca tgtcaaccgg atgtcatctg
     2521 cacttcgagg tcatggtcgg cggcgcgttc gtcgacccgg cgccgtatct cgacctggcg
     2581 ccggcaccca aggtggtcat tccggcagcc gtccccgccc gctccgatgt catcctggcg
     2641 ccagcaccga cgttgacgac gaccccgccg gtgaccccca ccctcacccc gaccgagact
     2701 gcggtgccga ccgagaccgt ggtgccgacc gccaccgcca gccccaccga gacggcgacg
     2761 acgagtccta ccgacgtgcc cacgtccacg gtggggccat ctcccacctc ggacccgacc
     2821 agcacggcga ccgatccccc gagcctgacg agtgatccga ccagcccgac atccgagccc
     2881 tcggcgccga ccgcgactcc cacctcggac ccggttcagt cgtcgcccac ggctacggat
     2941 ccggcgtcgc cctgacgctc cctcgaggga gctcgacgac ggcttcggcc gggtgctggc
     3001 tcaggccagc cgggcggcga tcgcgtcgcc gatggccacg gtcgaacgca cctggttccc
     3061 gcgctcgacg aggtcggcgg ccactgccgc ttcgacccgg gcagcggcgt cggccagacc
     3121 gacgtggtcc agcatcatcg ccaccgagag gatcgccgcc gtggggtcgg ccttcccctg
     3181 gccggcgatg tccggcgctg acccgtgcac cggctcgaac atgctcgggg tgctgcgggt
     3241 cgggttgatg ttgccgctgg cggccaggcc gatcccgccg gcgatggccg cagcgatgtc
     3301 ggtgatgatg tcgccgaaga ggttgtcggt gacgatgacg tcgaaccgac ccgggtcggt
     3361 ggcgaggaag atcgttgcgg cgtcgacgtg ctggtaggcc gtctgcacct ccgggaactc
     3421 cttcgcgaca tcctcgacgg tgcgccgcca caggtggccg gcgaaggtga ggacgttgtg
     3481 cttgtgcacg agcgtgaggt gcttgcgggg gcggccctgc gcgcgggcga acgcgtcgcg
     3541 aacgacccgc tcgacgccga acctggtgtt gacgctgacc tcggtcgcca cctcgtgcgg
     3601 ggtgccggcg cgcaggaggc caccgttgcc gacatagggg ccctcggtgc cctcgcgaac
     3661 gacgacgaag tcgatcccgt cgggggcgac acgcgcgatg tccaacgggc tggcgacccc
     3721 ggggtagagc ttggccggac gcaggttgac aaagtggtcg agcgcgaacc ggatcggcag
     3781 cagcacgccg cgctcgagga caccgctggg cacggtcggg tcgccgatcg ctccgagcag
     3841 gatcgcgtcg tgcccgcgca actcggccag gaccgagtcc ggcagcgtct caccggtcgc
     3901 gtgccaccgg cgggcgccga ggtcgtactc ggtcgtcgag atggacaccg ctcccccggt
     3961 caccgcggcc aggaccttga gaccctcacc cacgacctcg gggccgatgc cgtccccacc
     4021 gatgacggcg aggttgagag aggtgggctc aggcgttgtc acgctggtgt cgaccatggt
     4081 cccagcctag gagccgtctt gccggctggg acctacgtct cagatctcga accgaccgtg
     4141 gcacggatcg gccgcgggca gatcagaggt tgacgacggc gaagtgcacg gccccgatgc
     4201 cctcggcgat ccgggcggcg acctcggccg gcaactcgga gtcgacgcta agcaccatga
     4261 gggcggtgtc gcgcttttct gaacgggcca cctgcatgcc gccgacgtta atcgccgagt
     4321 cgccgaggat gccgccgacc tggccgatga tccctgggcg gtcggcatac gtgaaggcgg
     4381 cgaggtggtc gctgagcggg acctcgaggt cgaagccgtt gatcccgacg agcttctcga
     4441 tcatccgcgg cccggtgagc gtccccgcga ccgacacctc ggtgccgtcc ttgagcgtgc
     4501 cgcgcaaggt cgtgacgttg cggaagtcgc cgctggccga gtcggtcgtc agcgtgacgc
     4561 cgacgccgcg cgcctcggcg atcaccggcg cgttgacgta ggacacctgg tcctcgacga
     4621 catcggtgaa caggcccttg agcgccgcga gcttccagat gctcacgtcg tgctcggtga
     4681 tctcgccgca cacgtcgagg tccaggtgca gcggcaccgc accggcgacg gccgtgaaga
     4741 cccgcccgag cttctcgacc aaagcgatcc cgggacggac ctcctcgacg atgacgccac
     4801 cggagacgtt gaccgcgtcg gggacgatct cgccgccgag cgcgaggcgc accgacttgg
     4861 cgacggcgat gccggccttc tcctgggctt cctcggtgga ggcaccgagg tgcggcgtga
     4921 cgacgacctg ctcgaacgcg aacagcggcg agtcggtgca gggctccttg acgaagacgt
     4981 cgatcccggc gccggcgacc cggccctcgc ggatggcgtc ggccagggcc acttcgtcga
     5041 cgatcccgcc gcgggcggcg ttgacgatgt ggaccgtcgg cttgaccttg ctcaaagcgt
     5101 cgacgccgat gaggccgagc gtctccttgg tcttgggcag gtgcaccgtg atgaagtcgg
     5161 cctgggcgag caactcgtcc aactcgacca gacgggcccc gagctgaccg gccttggcgg
     5221 gggttgtgta ggggtcgtag gccagcacct gcatgccgaa gcccttgagc cgctcggcca
     5281 cgagggcgcc gatccgaccc aacccgacga tgcccacggt cttgtccagc agttcgacgc
     5341 cgccgtactt gctgcgcttc cacgccccgg ccttaagcgc ctggttggcg ggggcgatgt
     5401 tgcgggccga agcgagcaac aggcccacgg cgagctcggc ggcggaggtg atgttggagg
     5461 tcggtgcgtt cacgaccatc acgccggcct gggtggcggc ggggacatcg acgttgtcca
     5521 gaccgacccc ggcccgggcg atgaccttga gccgcttggc ggcggagatc gcctcggcat
     5581 ccatcttggt cgccgatcgg atgagcacgg cgtcgacgtc ggccagggcc ggcagcagca
     5641 cggctcggtc ggcaccgtca cacgaacgga tctcgaagtc aggaccgagg gcatccaggg
     5701 tggcgggcga cagttcttcg gcgatgagga ccacgggctt ggtcacgggg gtttccttcg
     5761 tggggcccgg cgcacggcga cgccggtggg gtggtgttcc gaggcacggc ggcgtgcccc
     5821 cgaaagtcta ggacgacccg cctggcgact cgcggggtgc ccacccaccg aacgtcgcga
     5881 gcgggtctca gtgcgtgcgg cctacttcgc cccgaggtcc tcacgcaact ggtacttgag
     5941 aaccttgccc gtgggattgc gtggcaacac ctcgacgaga tgcagccgtt ggggcagctt
     6001 gaagcgggcc aggtgggagg cggcatactc gcgcaggtct tccaacgtga cgctctggcc
     6061 gggtttggcg gcgacgacag cggtgaccgc ctcgccccaa cgggcatcgg gcaggccaat
     6121 gacggcgacc tcggcaaccg acgggtggcc gaacaacacg ctctcgacct cggcgggata
     6181 gacgttctcg ccgccgctga tgaccatgtc cttgacccgg tcgacgatgg agatgatgcc
     6241 gtcctcgtcg aactgtccga catcgccggt gtggaaccac ccagcctcgt cgaaggcggc
     6301 ggccgtggcc tccggccggt tccagtaacc ccgggtgacg ttcgggccgc gcatgcacac
     6361 ctcgccgcgc actcccggct cgctcaccgt gaccccgccc gagcccacga gcttggtctc
     6421 gatgtagagc ggcggctgcc cggcggcacc gagtttggac agggcgtatt cggcgacgag
     6481 gaagatcgct gcaggcgagg tctcggtgag gccatacccc tgcagcacgc cgatcccccg
     6541 atcggcgaag gtccgcagca gcggctccgg ggcgggcgcc ccgccgcaga cagccatccg
     6601 caccgtcgac aggtcggcgg cctggaagcc cggcaactgg ctgatcgcga tgaacatcgc
     6661 gggcaccccg aagagggtgt tgacccggta gcgggcgatg tcctcgaggg tccgttgggc
     6721 gtcgaaagac cggtgcagga tgacctctcc cccgcgcatc cacgtgatgc cgatcatgaa
     6781 gttgagcccg gcgatgtgga acagcggcga gaaggccagc gtcacgtcgt cggcgtggat
     6841 gtcgatccaa ctcatgaggg cgatgtcgct ccaccagagg ttctcgtggc tgagcatgac
     6901 gcccttgggg gtgccggtgg tccccgacgt gtacatgatg atcgacaact cgtccgggcg
     6961 aaccgggtcg ggtgtcggca acgggtgcgc cgcagtcacc gcctgctcat aggactccca
     7021 cccctccggt atgccgtccg cctcggtcac gaggtaacgc cggactccca gctcggcccg
     7081 gatcgcgtcg atgcccggcc gttggtccgc ccctgcgatg agggtgtgaa ccccggcgtc
     7141 cccgacgatg aaggccaact cggggccggc caaccggaag ttgagcggca catagatcgc
     7201 cccgagccgc gagcaggcga accaggtctc cagcgcggcc ggctggttga agccgacgaa
     7261 cgagacccgg tcaccgtgct tgacgccgcc ggccctcagg accgcggcga ggcgttcgat
     7321 ccggtcggcc aactccgcga acgtccagct cgtgccttcg aagatgagcg cccggcgttg
     7381 gggcgaacga gcggcgcggg cggcgaacca cgcaccgacg gtgatgtcag gcatcgacat
     7441 atgcagcact cccttgtgca tcggtaatca atcaacccac cagggtgttg tcccctggat
     7501 gttgcgtggc caggcgttcc aggagatggc gcaaggtgcg ttgctcggcc ggtgagagga
     7561 ccgccagcca ctgcccgtcc aggtcgtgct gggcctggaa ctcccgctcg accgtgcgcc
     7621 gcccgtcgtc ggtgaggcgg acctccacca ggcgccgatc ccttccctcg ccgcgctcgc
     7681 ggcataccaa accacccgac tcgagcgacg ccaagaccgc ggacgtggtc tgtcgactca
     7741 tcccgagcag ccgggcgagg tcgcgcgccc tcagccggcc gtagagccac acgaggtaga
     7801 gcacgctgaa cgcgcgcagg tgccgcccgc cctggtcgtg agcgcgccgc tcgaacagtc
     7861 cacgatggcg accacccgcc cgcaacagcg ccacgacgag cgccgtcgtc tcggggtcgg
     7921 cgccctcggc ccggcgttga gtgaggtcgg cgaccgtgtc gacgaagtca ctcgcggccg
     7981 ggcgcgggcg atcgtgcggc tccccgagca tctgcggcct ccggtcaggt cgcgtcgccg
     8041 accgcagccg gcgcgacggc ggccagtcgg gccgatcctg gcccggcccg atgcggcgtg
     8101 cgcttgcggg ccgccttggc ctcggcagga gcagccttcg ccgaggagtc accggcagcc
     8161 gcgaccgtct cggccaacgc gtcggtcatg tagccgatca gcggccgcag gtcgccgacc
     8221 tgttcggggg cgcccatgaa gccgaagcag atccggtcgc tgtagctctg cagggtgatg
     8281 ttgatcccgc caccgacgga caggaacgac gcgggccagt agcccttgat cttgaccccg
     8341 tccatgtatg ccgcggtgct gggtccgcgg acgttggaaa cgacgacgtt ccacgggccc
     8401 ttggacacct tgtcgggcat cttgaccatg gcccgcgtca tgacgttgaa catcgccgag
     8461 gggatgaacc ggctcgcctc gcggatcaac ccgaccggca gcgcgtcgaa gttgcccttg
     8521 gcggccttga ccgcggcgcc ggcagcccgc acccgggcca gcgggtcatc gaggtgcgtg
     8581 ggcagcggcg cgaagatcat gtggacgtag ttggcccact tcgtcgtctc ggcgccggtg
     8641 cggatcgaga cgggcacgca cacgatgagc gggtcgtccg tgggtatgcc gttctccacg
     8701 aggtagcgac gcaaggcacc ggcacacact ccgaggacga cgtcgttgat cgtgccgccg
     8761 agggccttgc cggtgtcgcg gaactgcgcc aacggcaggt cggcgaagac gaaggtgcgt
     8821 tgggcggtca cccggttgtt gaacggcgtc gtgggcgtac gcagggtggg gatgtagggc
     8881 tggatctccg cgatcccccg cttgctcagc gccgagttga acagggcctg ggccggctgg
     8941 gcgaggccgc ccggcaggct gacccggaac ggcagggcga gcggcgccaa gggtcccttc
     9001 tccttggtct ggccagcgac ccaggcgagg aacttggcct ggaaggcggc gaactgcacc
     9061 ggcttggcga ccactccgcc cagctcgcgg gcgagcatct cgcccttgcc gaagcgcggc
     9121 tgcgggcgcg tgctctcggc ccatgcctcc aacggcgcag ccggctcgtc ggacagcgca
     9181 tcccacagcg tcggcatcga ccctccgtcg atgaccaagt ggtggatccg cagcaggtgg
     9241 gcgacgctgc cgtcggccag cccgtcgatg acggtgtagt cccacagcgg gcgccctgtc
     9301 ggcagcgacg tgctcatgat ccgcgagacc tcggcggcca gctggtcgtg ggtgcccggc
     9361 gctgggagcg agatggagcg gatgtgctcg agcaggtcga tccggtcggg ctggccaagg
     9421 tagtcgtggt cgatgccgat cggcaccttg accacctctc ggtgcagcgg cggcaggaag
     9481 ggcaaccgct cggcgaaccg ttgacgcatg aacgccgcgt cggcggccgg gcggccgtcg
     9541 gctgccggct cgtgaagcac cagcccgccc aggacaccat tggtcgtttc ggtgtccagc
     9601 gaggagaaca tcacatcgag ggcggtcagt tgctgcacgg aacctccgga cgtagggacg
     9661 ggcatgacgg tcgcagacag tcgttggtaa ccacaatgag tctacgcttg gactgtcagt
     9721 gatttgacag gttactgggc gcgggtgtgg ccaggaatag cgaagcaagg aaattccaca
     9781 gtgcggtacg ccaggctccc cccgcccccc agcgtgaccg ggctcagtcg gcggcgggag
     9841 tctcggccgg gcggtcgatg cgggtcaaca cgcgttcgag catgtcctgc aactgcgcct
     9901 gctcggcact gttgagcacc gagaaccaat cactctcgag cgcgttctgg gcgccgatcg
     9961 cctcgtcgac cgcggctcga ccccgaggcg tcagccgcac cgagaccagg cggcggtcgg
    10021 tcttggaggt ctgccgacgc acgaccaatc gggccgcctc gagggtcgaa agcaccgtcg
    10081 aggtggtctg gcgactcacc ccggcgaagc gggccaggtc gcgtgcctcg accggctcgg
    10141 tgagccagat catgtagagc acccggaagc cgctgtagct ccacccccgc tcacggtgca
    10201 cgagccgctc ggattggtgc tgatgggtgg ctgcgatcct ggccaaggtg aggctcagag
    10261 cgatggctcg cgggtcgaca tcgggccgtc gttcctgggt cgtgcgcacc gctcgggctc
    10321 ggaactcggc gcccggcacg tccgaagccg gggtctggct gcccatcatg tgcctcgttc
    10381 tgcagataag aatgcttgtc agcaccctac ccggcggtag ccagccacgc gcagcggtgg
    10441 gtcagtccct cccaccggag tccgccacgt ccgggaacga ggcataggga ggcgtcgact
    10501 cgagccgtcg gttggtcagt cgggtggcgc ccacgacgag ggcgatcagg cctatcgcgg
    10561 cgccgagcca gagccagggc gactggctca cccacgcctc gaagaccgcg aacccgatcg
    10621 tggagtagat gccggcccac atcagggcgc cgatgacgac gccgacctcg tagcgccaca
    10681 acggcattcg cagcacgccg gcggcgaagt tgagcgctgt ctggactccg accgtgagga
    10741 aggacagcgc cacggccggg gccccgaacc gcgaaattac cgtctcggct cgccgaatgg
    10801 ccgggctgtg cagccgtcgc gccgcgcggg tggtctcccc gcccttgcgg gcgccgcgcc
    10861 ccagccagta ggtcgcgttg gcccgaatca tggcgccgag gaagaacacc gcatagacca
    10921 gcgggaaggg caggccgtcg aacaccacgt caccgaacgg gtatgccgcg gcgcatctcg
    10981 cgcacgcggc atacctcgct gggaaagatc atcgggtcag gctagcccac ctgaggcccg
    11041 tgattgaaag gtcacggaag gtgactgaag ggtcaccgag cagcggagcc ctcggtgtag
    11101 tcggagtccg tctccttgat ccagctcatc agcttgcgca gatcgcggcc ggtcgcctcg
    11161 atcgggtgct gagcaccctt ggcgcgcagc gccttgaact ccggagcccc agcgtcctgg
    11221 tcggcgatga accgacgggc gaaggcaccg ctctggatgt cggtgagcac cgcgcgcatg
    11281 ttgtccttga cgctcgggtc gatgacccgg ggcccggaga cgtagtcgcc gtactcggcc
    11341 gtgtcggaca ccgaccaacg ctgcttggcg atgccgccct cgaccatgag gtcgacgatg
    11401 agcttgagct cgtgcaggca ctcgaagtag gcgacctcg
//
LOCUS       DAOMZY010000068        11435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5304_length_11435_cov_6.109842, whole genome shotgun sequence.
ACCESSION   DAOMZY010000068 DAOMZY010000000
VERSION     DAOMZY010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 11435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11435
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5304_length_11435_cov_6.109842"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1197)
                     /locus_tag="PLK64_05555"
     CDS             complement(1..1197)
                     /locus_tag="PLK64_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPV79240.1"
                     /translation="MPRWDDRLVDDEAERERLDSRGTLRALATAGAQVRQSLGLAQEA
                     GIARVAGGVRPRGVLVAARGGTGVIGDTLQMVAEPGSPVPISTAGTPPLPGWVGTLDL
                     VIAVSESGRAAGPLALAAAAARRGASLLTVGADDSPLAAVCASARGIHVGLPAGATSS
                     RTALWALLTPVLLAADVLGLARTGPDLLEAVADRLDAGAAAYRPSSEAFVNPAKLLAA
                     DLSGGLPVVLGDGAVAGVAARRAAMMLARTARVPATVGELPGSASEVVALLDGPYTRH
                     GAREPDLASAVDDIFRDPFLDGAPDPRLVLLMLRGALAAGMTPAGVGGSMPEDTDDLG
                     DAVATAAMDAGVRVHERRAEPGHPLEQLADLIGLVDFAATYLALGLGLDPGVSAHVRA
                     LRDHGR"
     gene            complement(1485..2924)
                     /locus_tag="PLK64_05560"
                     /pseudo
     CDS             complement(1485..2924)
                     /locus_tag="PLK64_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
     gene            complement(2969..3364)
                     /locus_tag="PLK64_05565"
     CDS             complement(2969..3364)
                     /locus_tag="PLK64_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011691057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 domain-containing protein"
                     /protein_id="HPV79241.1"
                     /translation="MGLTRQCSKTGCRRSAVATLTYVYADQTAVLGPLATYAEPHTYD
                     LCGQHAERLTVPRGWDVVRLAFDPSPPAPSSDDLLALADAVREAGRPRSRAPLSPPSS
                     PSQSSAPDAAEASPATGARRGHLRALPNN"
     gene            3466..3897
                     /locus_tag="PLK64_05570"
     CDS             3466..3897
                     /locus_tag="PLK64_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HPV79242.1"
                     /translation="MNRDDPRAPRTRRRHGRGVRGPLAWPPVPAMRSRAERFDELVMD
                     AATRFEGHWGRPFPAFELGVEDVPPSDPAPWEHEEVPLGRFFASDARREARVVIYRRP
                     IETRAQAPAELALLVDEVVAEQLAALLGVSPRDLDPGYDGD"
     gene            complement(3906..5396)
                     /locus_tag="PLK64_05575"
     CDS             complement(3906..5396)
                     /locus_tag="PLK64_05575"
                     /inference="COORDINATES: protein motif:HMM:NF040160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5719 family protein"
                     /protein_id="HPV79243.1"
                     /translation="MTPIGWSALRVLGFAAVLGGLVAGSTQLPSRLELTSIAPVVPAD
                     VLSPPSTPASQMTLGCPGPETDGFSGLPAVPGEATSILAATAPSEAMQGIATAPAPGA
                     VAVRSSASAASDPALATSATRGGVVSGDVSGAAAGEVSATGSLAVGLAALQTGLRREG
                     DERGLQSVACQAPRSEVWLLAGGGAPTRRERLVVANAGANPVTVDIQVHGASGPIASV
                     NGARVAVPPHGRVGVLIDAIAPGEASPVVHVTATGGLITAVLEDSWIDGATGRGRDDV
                     GPADGPATEQVIPAAYLTGVATLRIVVPGADEAVVQSRALSPNGAVPLPADQVVRIPG
                     GSVRDIDLSGLEPGAYALQVRADRPVVAAVVIERRDAGVAQSDLAWVPATAVIPVLAG
                     SPVPAGTVSRLLLAGTAAPWAATVYVVGPSGAVTSIPAQGVADSSWTVDLPAGAAAVW
                     IRPSSGTVRAGISAELADPTGPLVTLVGINPATLTTTAIPVREVRR"
     gene            complement(5393..8659)
                     /locus_tag="PLK64_05580"
     CDS             complement(5393..8659)
                     /locus_tag="PLK64_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPV79244.1"
                     /translation="MTAATPDPAVSTPTDPTPTASEPTPVARRPFVTAVALCRSGVDS
                     LTVTLAALADQTRRVDRIVVLDLTQGAADLGQVTRTQSGADTGLPEAELVSEPTAGPV
                     GAAVNRLLQTLAQAPDPAAEWWWLLPDDVVPEPAALAKLVDAVRRSPSVGMAGPKVLD
                     RNRPRLLVEVGHQLTRSGRRIAAPGRGEPDQGQYDTRTDVLAVGLPGQLVRREVLTQV
                     GGFEPAFAGPGEALDLGWRAQLAGERVVVVPAARVLAGPETSTTETPQGPEPTVPAET
                     MSERRAARRAARRVALTRCSPWAVPGLALWVLLSSIGSALVLLLLKRPAHAWVELADL
                     GALVRPFSSIAARWRFRTRRRLRRRDLATLFVTSGESLRHTADRVQEALTPDRDGARA
                     GLAAGAGIESGPVAEEAEDLNVLSASLPERVFSNAGVLATLAAGIVAAVGFRSSFRGG
                     LLDARGTGLAGGELARVSTDASGLWHAFRDSWHGAGLGTGADSAPYLGVLATLTWLVE
                     RLPYVGEGRSPASVLLAWILVLGMPVATAAAYLAGRVVTPARWPRALVAFAWGTGGVI
                     AAAVSHGRVTLVLAHILLPLVVAGVVRSARADGTFTAAAAAALGAAVLGAMVPPYLAL
                     VAVAAAVLVIVGPGWGRRVRGLALLVLPPALQGSLLVQLRDPVAWLGAPGALDSAGAT
                     TASWWQLLAGYPEGGSALVVALSAPVLLAGVVTLVRRPGSRGAGVAYAALTLLAAVGL
                     GWALLARRVVVGTVLGADGVPVEATPWPGVGLQCFVLAVLGLALLGSVGMRAVLGKER
                     WAPRRLLAGSGLLVVTASVAGVAALGGWAHFDGIVSVGFDALPAVAVEQARGPEANRM
                     IVLSTSEAAVEYQLVAREPGEILRGLARPSGASDPGVAAAVTALVASQAGDAPGAGAQ
                     LAELGIGFVSVQGSADPEVIRTLDATAGLTRLGNTDDQTLWRVLARPSVTTPTEVVPA
                     SRARITTADGGLSEWVRVDGAHGALTASLPAGSVGRRLVLAEPREWVQHAQVHFDGAL
                     LTATSQGLPTYVLPDRAGVLTVDIPPRDPRWFAGQFALLALTVFLAVPFGNRRSRRIR
                     "
     gene            complement(8703..8966)
                     /locus_tag="PLK64_05585"
     CDS             complement(8703..8966)
                     /locus_tag="PLK64_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HPV79245.1"
                     /translation="MHELLLVPMDGFQDEEMGWQERSLCAQTDPEAFFPEKGGSTREA
                     KRVCVGCEVRVECLEYALANDERFGIWGGLSERERRKLKKRAV"
     gene            9226..10200
                     /gene="cofD"
                     /locus_tag="PLK64_05590"
     CDS             9226..10200
                     /gene="cofD"
                     /locus_tag="PLK64_05590"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HPV79246.1"
                     /translation="MRVTALSGGVGGARFLRGLLAHIDRTPELAGSTVTVIGNTGDDI
                     TLFGLRVCPDIDTVLYTLGGGVHEAQGWGRSDETYAVHAELGALGVPPDWFSLGDNDF
                     GTHIARSLWLGQGLSLSQVTARLAQRWGLPDRGVTLLPMTDTPVETHVVVDEGEGPRA
                     VHFQEWWVRMRAAVPAERFVVAGMDRATPAPGVLDAIRTADVVLLPPSNPVVSIGIIL
                     GVPGVRDALRGTRAPVVGVSPLVSGRPVRGHADACLSAIGIPATSAAVATLYADFLDG
                     WLVDDSDADVAMPDGVRTLARPLLMTDIPAAADIAGAALDLGRDLRGR"
     gene            10197..11372
                     /locus_tag="PLK64_05595"
     CDS             10197..11372
                     /locus_tag="PLK64_05595"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HPV79247.1"
                     /translation="MTASLGSGAVTITPLHGIPEIAEGDDLAAVLAAACVRAGVPLAE
                     GDILAVSSKIVSKALGLTAPTADKEAVVAAETVRVVAERAGASGITRIVQGKAGPVMA
                     AAGVDASNTGGRDVLLLLPHDPDAVCRDLHAALRAATGVEVFAVVLTDTAGRPWREGQ
                     VDFALGSHGLRVLDDLRGSVDADGRALEVTARAVADEIAAAADLVKGKTAAVPAALVR
                     GLAASVLPVVNDREGVGMAQAGSSPQAGASRLVRVGDGDWFALGSQEAVRAALGVPPG
                     PGSGSAHGPRPVAGDTLARRVIRALDVAFRPHLDDWHGGVAVAGPPGTVAGERVGDLV
                     VSGSNAFRVGVVAARLQVALRGEGVAFEVDPEPTVDAAGEWRATLRIGDGIVGESAG"
BASE COUNT         1693 a         4095 c         4227 g         1420 t
ORIGIN      
        1 tcagcggccg tggtcgcgca gcgcccggac gtgcgccgaa acccctggat ccaggcccag
       61 gcccagcgcg agataggtcg ccgcgaaatc gaccaggccg atgaggtcgg ccagttgttc
      121 caacgggtgg cccggctcgg cccggcgctc gtgaacccgc actccggcgt ccattgctgc
      181 cgtcgcgacc gcatcgccga ggtcgtcggt gtcttcgggc atcgagccgc cgacgcctgc
      241 gggcgtcatc ccggcagcca gcgctccccg caacatgagc aggaccagtc gcggatcggg
      301 ggcgccgtcc aggaaggggt cgcggaagat gtcgtcgacg gccgaggcca ggtcgggctc
      361 gcgggctccg tggcgcgtat agggcccgtc gaggagcgcc acgacctcgg aagcgctgcc
      421 gggcaactcg ccgaccgtcg ccggcacgcg agcggtccgg gcaagcatca tcgcggcccg
      481 gcgggcggcg acccccgcca ccgcgccgtc cccgagcacc acgggaagtc ccccggacag
      541 gtcggccgcc aggagcttgg ccgggttgac gaacgcctcc gaggacggcc ggtatgccgc
      601 cgcaccggca tcgaggcggt cagccacggc ttcgagcagg tcgggtccgg tcctggccag
      661 gcccaacacg tcagcagcca gcaggaccgg cgtcagcagg gcccagaggg ccgtgcggct
      721 ggacgtcgca ccggcaggca gcccgacatg gatgccgcgg gccgaggcgc agacggcggc
      781 caacggcgag tcatcagcgc ccacggtgag cagcgatgcc ccgcgtcggg cagccgccgc
      841 agccagggcc agcggtccgg cggcccgacc cgactccgac accgcaatga ccaggtcgag
      901 ggtccccacc caccccggaa ggggcggtgt ccctgccgtg ctgatcggca ccggagaccc
      961 cggctcggcc accatctgca aggtgtcacc aatgaccccc gtgccacccc gcgcggcaac
     1021 gagcaccccg cgcggacgaa cccctccggc gacgcgggcg atgccggcct cttgggccaa
     1081 cccgagcgat tggcggacct gggctccggc ggtggccagc gctcgcagcg ttccccggct
     1141 gtccagccgc tcgcgctcgg cctcgtcgtc gacgagtctg tcgtcccagc gcggcatacc
     1201 ggctcctcct tggggtgcga acccgaccga gatcgggcgt ctgctcagac cggcttgcgg
     1261 gccagatcgg caagcaggac ggggatcccg ccctcgatcg gataggccaa cccgcactcg
     1321 ggtgaggtgc agaccaactc aggaccggct gggccggtct cgtcgcgcag cgactcgcga
     1381 caggccgggc agcgcaggat ctcgcgcagc caaggctcca ggctggccgc ctcctcagac
     1441 ggctgtgccg gggccgccgc agcgtccggc actgctggcg ccgactggcg cagcaggccg
     1501 aggacgcagt cgcggatcga agccatcgtg gcggcgtcgg ccgcctcgac gttgaggcgc
     1561 agcaacggct cggtgttgga ggctcggacg ttgaaccacc acatcggctc cgcgaggtgg
     1621 cgcaccgtgg tgccgtcgag ggtgtcgatc tcgaccggcc cctgcgcgcg cgcccagtcc
     1681 agcgcacgcg tcgtcgcggc ctcccggtcg gcgaccgtcg agttgatctc gccggacgcg
     1741 gcatacctct cgtatgccgc cccgagcacc gacagcggtg cgtccgactc cccgagggcg
     1801 gccaacacat gcatcgctgc gagcatgccc gtgtcggcga accagaagtc gcggaagtag
     1861 tagtgagccg agtgttcgcc gccgaaaacc gctacagccg aggccatctc ggccttgatg
     1921 aaggagtgac ccacgcgcgt gcggacgggc acggccccaa gctcggtgac gatctcgggc
     1981 acggcgagtg acgtgatgac gttgtggacg atccggacct ggctcacatc gtcgccgcct
     2041 gcgatggccc gggcgatctc gcgagcggcc acgagcgccg tgatggcgct ggggctcacc
     2101 ggctcaccgc gctcatcgac gacgaagcac cgatcggcgt caccgtcgaa ggctaggccg
     2161 atgtcggcac cctgctcgac gacggcacgc tggaggtcac gcaggttctc cggctcgagg
     2221 gggttggcct cgtggttggg gaaggtgccg tccaactcga agtagagcgg aatgacctcc
     2281 aggggcagtt ccgccagccc ggcgcccgtc tcgaggaccg ccggcacggt gtgtccaccc
     2341 atgccattgc ccgcgtcgac gacgacccgc aaccggcggt tgccggacag gtcgaccagc
     2401 gaccgcagga aggctgcgta gtccgccaac acatcccggt gagtgaccgc tccgaggcgc
     2461 cccgccgcgt cggtggccgc gacggcggca ccatcggtga cagcgttgtt gagcagtgtc
     2521 tgggccaggt cgcggacctg ggccaacccg gtgtcctgac cgacggggcg agccccggcc
     2581 cgacacatct tgatgccgtt gtactgcgcc gggttgtggc ttgcggtgaa catcgcgccc
     2641 ggcaggtcga gggcgccact cgcgtaatag agctggtcgg tcgaggcgag gccgatgatg
     2701 atgacgtcga ttccggtctc ggcgacaccg tcggcgaacg cagccgacaa ctccggcgag
     2761 ctgggtcgca tgtcgtagcc gaccaccacc gttgcggccc cgtcggggag cgcgacgatc
     2821 tcggcgaagg cgcgcccgag ggcacgggcg acggtggtgt tgagttgggc cgggaccacg
     2881 ccgcgcacgt cgtatgcctt gacgaactgg gtcaggtcag ggatgtcacg gctcgtcacg
     2941 aacggcagcc tagcgcggtg gccaggggtc agttgttcgg cagggccctc aggtgacccc
     3001 ggcgtgcgcc ggtcgccggg gaggcctcgg ccgcgtctgg ggcgcttgac tgcgaggggc
     3061 tcgaaggggg gctcaatggg gcgcgcgagc ggggccgtcc ggcctcgcgg accgcgtcgg
     3121 cgagggcgag caggtcgtcg gatgagggtg ccggcgggct cgggtcgaag gccagacgga
     3181 ccacatccca cccccgcggg acggtcagcc gttcggcgtg ctgcccgcac aggtcgtagg
     3241 tgtgcggttc ggcataggtg gccaaggggc cgagcacggc ggtctggtcg gcatagacgt
     3301 aggtcagggt ggcaacggcc gaccgccgac accccgtctt ggaacactgc cgagtcaagc
     3361 ccacgcgggc actctaaacc cgggccgggt gtatgccgcc tcggcgcgcc gcagccggcc
     3421 cgcggacccg ggcacacggc atacccgcgc cgtagagtgg agcgcgtgaa ccgtgacgat
     3481 ccccgcgcgc cgcgcacccg ccgccgccat ggccgcgggg tgcggggtcc gctggcgtgg
     3541 ccgccggttc cggcgatgcg cagccgcgcg gagcggttcg acgagttggt catggatgcg
     3601 gcaacccggt tcgaagggca ctggggacgg ccgttcccgg ccttcgagct gggagtcgag
     3661 gacgtgcccc cgagcgaccc cgccccgtgg gagcacgagg aggtgcccct cggtcggttc
     3721 ttcgcatccg atgcccgccg cgaggcccgc gtcgtcatct atcggcgacc catcgagacc
     3781 cgtgcgcagg cacccgcgga gttggccctg ctcgtcgacg aggtcgtcgc cgagcagttg
     3841 gctgcgctgc tcggtgtgag cccgcgcgac ctggatcctg gctacgacgg cgactgagcg
     3901 cgccgtcagc ggcggacttc gcgcaccggg atggcggttg tcgtcaaggt ggctgggttg
     3961 atcccgacga gggtcaccaa gggcccggtg gggtcggcga gctcggcgga gatcccggca
     4021 cggaccgtgc cggaggacgg ccggatccag acggccgccg ccccggccgg aaggtcgacc
     4081 gtccacgagg agtcggccac cccctgggcc gggatcgacg tcaccgcgcc cgaggggccg
     4141 acgacgtaga ccgtggctgc ccatggcgca gcggtgcccg cgagcaacag tcggctgacg
     4201 gtgccggcag gcacgggaga gccggccaga acggggatga cggccgtggc ggggacccag
     4261 gccaggtcgg actgcgccac gccggcgtcg cgccgctcga tgacgacggc ggccacgacc
     4321 gggcgatcgg cgcggacctg cagcgcgtag gcgcccggct ccaaccccga caggtcgatg
     4381 tcacggacac tgccgcccgg gatccgcacg acctggtcgg cgggcagcgg aacggcgccg
     4441 ttggggctca aggcacgcga ctggaccacg gcctcgtcgg ccccggggac gacgatccgc
     4501 aaggtggcga cgccggtgag ataggcggcc gggatcacct gctcggtggc cgggccgtcg
     4561 gctggcccga catcgtcacg accacgcccg gtcgccccgt cgatccagct gtcttcgagc
     4621 accgctgtga tgaggccacc ggtggcagtg acgtgaacga ccgggctggc ttcgccgggg
     4681 gcgatggcat cgatgaggac gcccacgcgg ccatgtgggg gcacggcgac ccgcgccccg
     4741 ttgaccgacg cgatcggacc ggacgcaccg tgcacctgga tgtcgaccgt caccgggttg
     4801 gccccggcgt tggccacgac cagccgctcg cggcgcgtgg gagccccacc accggcgagc
     4861 agccacacct ccgagcgggg cgcctggcaa gccactgact gcagaccgcg ttcgtcgccc
     4921 tcgcggcgca gcccggtctg cagggccgcc agccccacgg cgagcgagcc ggtagcgctg
     4981 acctcgccgg cggccgcgcc cgacacgtcg cccgagacga cgccgccacg ggtggccgac
     5041 gtcgcgagcg ccgggtcaga ggccgccgag gccgatgagc ggaccgcaac ggcgccgggc
     5101 gccggagcgg tggcgatccc ctgcatcgcc tcgctcggcg cggtggccgc aaggatgctt
     5161 gtcgcctcgc ccgggacggc cggcagcccc gagaagccat ccgtctcggg gccggggcac
     5221 ccgagcgtca tctggctcgc cggggtgctc ggcggggaga gcacgtcagc ggggacgacg
     5281 ggggcgatcg aggtcaactc cagccggctg ggaagctgcg tggacccggc cacgagtccg
     5341 ccgagcacgg cggcgaagcc gaggacccgc agcgcagacc acccgatcgg ggtcatcgga
     5401 tcctcctgga tcggcgattg ccaaacggca ctgcgaggaa gaccgtcaga gccaggaggg
     5461 cgaattgccc ggcgaaccaa cggggatcgc gcggagggat gtcgacggtc aacacgccgg
     5521 cccgatcagg caggacgtag gtcggcagac cctgcgacgt cgccgtcaag agcgctccgt
     5581 cgaaatggac ctgagcgtgc tggacccact cgcggggctc ggccaggacc agcctgcggc
     5641 cgaccgaccc ggcaggcagg gaagccgtca gcgcgccatg ggcgccatcg acgcgcaccc
     5701 actccgacag gccaccgtcg gccgtggtga tccgcgccct gctggccggg accacctcgg
     5761 tcggcgtcgt gaccgacggg cgggccagca cccgccacaa ggtctggtca tcggtgttcc
     5821 cgagcctggt gagcccggct gtcgcatcga gggtgcgaat cacctcgggg tcggcgctgc
     5881 cttggaccga cacgaagccg atgccgagct cggccagttg ggccccggcg cccggagcat
     5941 cgccggcctg gctggcgacc agcgcggtga ccgcggcggc gacgcccggg tcggacgccc
     6001 cactcggacg ggcgagcccg cgcaggatct cccccggctc acgggcgacg agttggtatt
     6061 cgacggcggc ctcgctcgtc gacaggacaa tcatccggtt ggcctcaggg ccacgcgcct
     6121 gctccaccgc gaccgcgggg agggcgtcga acccgacgct gacgatcccg tcgaagtgcg
     6181 cccagccgcc caacgcggca acgcccgcca ccgatgcggt gaccaccagc agaccggatc
     6241 ccgcgaggag ccgacgaggc gcccaccgct ccttgccgag gaccgcgcgc atgcccactg
     6301 agcccagcag cgccaagccc aacacggcga gaacgaaaca ctgcagcccg acccccggcc
     6361 acggagtcgc ctccacgggg acaccatcgg ccccgaggac ggtgcccacg acgacacgtc
     6421 tggccaacag ggcccacccg agcccgacgg cggcgagcag ggtgagggcg gcatacgcca
     6481 cgcctgcgcc ccgcgagcct gggcgacgga caagggtgac cactccggcg agcagcaccg
     6541 gcgccgacaa cgcgacgacg agggccgagc caccctccgg atagccggcg agcagttgcc
     6601 accaggaggc cgttgtcgcg ccggccgagt cgagggcacc aggcgcgccg agccaggcga
     6661 ccgggtcgcg cagttggacg agcaacgacc cctgcagggc tggggggagg acgagcaggg
     6721 ccaggccgcg cacccgacgt ccccaaccgg ggccgacgat gacgagcacc gcggcagcca
     6781 cagcgacgag ggcgagatag ggcggaacca tggcgcccaa caccgcggcc cccaacgccg
     6841 cagcggccgc cgcggtgaag gtcccgtcgg cccgagccga gcgcaccacc ccggcgacca
     6901 ccaagggcaa caggatgtgg gcgagcacca gggtgacccg cccgtggctc accgcggcgg
     6961 cgatgacccc gccggttccc caagcgaagg cgaccagcgc gcgcggccag cgggccgggg
     7021 tgaccacccg gccggcgagg tatgccgcgg cggtcgcgac cggcataccg aggacgagga
     7081 tccacgcgag caggacggac gcgggcgagc ggccctcgcc cacataggga aggcgctcga
     7141 ccaaccaggt cagggtcgcg agcacgccga ggtagggtgc cgaatcggca ccggtgccca
     7201 agcccgcccc gtgccacgaa tcgcggaacg cgtgccagag gccggacgcg tcggtcgaga
     7261 cgcgagccaa ctcccctccg gcgagaccgg tgcctcgcgc gtcgagcagc ccgccgcgga
     7321 acgacgagcg gaacccgacc gccgcgacga tgcccgcagc cagggtggcc aggacgccgg
     7381 cgttgctgaa gacccgctcg ggcagcgacg cagagaggac gttgaggtcc tcggcctcct
     7441 cggcgaccgg gccggactcg atgccggccc cagccgcgag cccggcgcgg gcgccgtcgc
     7501 gatcaggggt gagcgcctcc tggactcggt cagcggtatg ccgcagcgac tcgcccgagg
     7561 tgacgaacag ggtcgcgaga tctcgtcgac gcagtcggcg acgggtgcgg aagcgccacc
     7621 gagcggcgat cgaggagaac ggccggacca gcgcgccgag atcggccaac tccacccagg
     7681 cgtgcgccgg gcgtttgagc agaagcagga ccagggccga gccgatcgag ctgagcagca
     7741 cccagagcgc cagcccggga acggcccatg gggagcagcg ggtcagagcg acccgtcggg
     7801 ccgcccgccg cgcggcgcga cgttcggaca tcgtctcggc cggaacggtc ggttcggggc
     7861 cttggggcgt ttcggtcgtc gaggtctcgg ggccggccag gacccgggcg gcagggacga
     7921 cgacgacgcg ttcccccgcc aactgggcgc gccacccgag gtcgagggct tcacccggcc
     7981 cggcgaaggc cggctcgaaa ccgcccacct gcgtcaggac ctcccggcgg acgagttggc
     8041 ccggcaggcc caccgccagg acgtccgtgc gcgtgtcgta ctgtccctgg tcgggctcac
     8101 cgcgacccgg cgcagcgatc cggcgaccgg acctggtgag ttgatgaccc acttccacga
     8161 gcaaccgagg ccggttccgg tccagcacct tcgggccggc catccccacc gacggtgacc
     8221 gacgcacggc atcgaccagc ttggccaggg ccgcgggctc ggggacgacg tcatcgggca
     8281 gcagccacca ccactcggcg gccgggtcgg gtgcctgcgc caacgtctgc aacagccggt
     8341 tcacggccgc gccgacggga cccgccgtcg gctcgctgac gagctcggcc tcgggcagtc
     8401 cggtgtcggc accggactgg gtccgggtca cctggccgag gtccgccgca ccctgggtga
     8461 ggtcgagcac gacgatccgg tcgacgcggc gggtctggtc agccaacgcg gccaatgtca
     8521 cggtcagcga gtcgacgccc gagcggcaca gggccacggc ggtcacgaag ggacgccgcg
     8581 cgaccggggt cggctcggac gcggtgggcg tgggatcagt aggcgtggaa acggcgggat
     8641 ccggggttgc tgcggtcacg ggactcctcg aggcgttgcg gccaagggcc gcaagggcgc
     8701 gctcagaccg cgcgtttctt cagcttgcgg cgctcgcgtt cggacaaccc gccccagatg
     8761 ccgaaccgtt cgtcattggc cagcgcgtac tccaagcact cgacgcgcac ctcacagccc
     8821 acgcagaccc gcttggcctc gcgcgtggac ccccccttct ccgggaagaa cgcctcgggg
     8881 tcggtctgag cgcacagcga gcgctcctgc caccccatct cctcgtcctg aaacccgtcc
     8941 attggcacca acagcagttc gtgcatgtcg cctcctcgac cctcgttggc atgcacccac
     9001 ctgtcctgca accgttcgac gccctgtcag accacgctgc ggcccggtcg ggtgtgaacg
     9061 acactggtgg aattacacgc gtgtcatacc cgtttagtca agccgaatct gatatttgag
     9121 gggttttcgc cgctccccag gcgtctcgga cgtccgggcg ggcgctgcgg cgtaccccgt
     9181 ggacggccgg tccgtgccca cccggcgggc tgacagactt cccccatgcg tgttactgcg
     9241 ttgtccggcg gcgtgggcgg ggcccgattc ctgcgcgggc tgctcgccca catcgatcgc
     9301 acccctgagt tggccgggag cacggtcacc gtcatcggca acaccggtga cgacatcacc
     9361 ctcttcgggc tgcgggtctg tcccgatatc gacacggtgc tctacaccct gggcggcggt
     9421 gtccacgagg cccaggggtg gggacgctcc gacgagacgt atgccgtgca cgccgagttg
     9481 ggtgcgctgg gcgtgccgcc cgactggttc tccttggggg acaacgattt cggaacacat
     9541 atcgcaaggt cgctgtggct cggccaaggc ctgtctctga gccaggtgac ggcccggcta
     9601 gcccagcgct gggggctgcc cgaccgcggc gtaaccctgc tgccgatgac tgacaccccg
     9661 gtcgagaccc acgtcgtcgt cgacgagggt gaggggccac gagcggtgca cttccaggaa
     9721 tggtgggtgc ggatgcgggc cgccgtcccc gccgagcgct tcgtcgtcgc agggatggat
     9781 cgggccacgc ccgcgccagg ggtgttggac gcgatccgca ccgccgacgt cgtcctgctc
     9841 ccgccaagca acccggtcgt gtcgatcggg atcatcctcg gcgtgcccgg ggtccgcgat
     9901 gccttgcgcg gcacccgagc gcccgtggtg ggcgtctccc ccctcgtgtc ggggcgcccg
     9961 gtgcgcgggc atgccgatgc ctgcctgagc gccatcggga tcccggccac ctccgccgcg
    10021 gtcgccaccc tctatgccga cttcctcgac ggctggctgg tcgacgactc cgacgccgac
    10081 gtggcgatgc ccgacggggt gcggaccctg gcccgcccac tgcttatgac cgacatcccg
    10141 gccgctgccg acatcgccgg ggcggctctc gacctcggtc gcgacctgcg cgggcgatga
    10201 ccgcctccct cggatccggc gcggtgacga tcaccccgct gcacggcata ccggagatcg
    10261 cggaagggga cgacctcgca gccgtgctcg cggccgcgtg tgtgcgggcc ggcgtgccgc
    10321 tggccgaggg cgacatcctc gcggtatcga gcaagatcgt gtcgaaggcg ctcggcctca
    10381 cggccccgac cgccgacaag gaggcggtcg tcgccgccga aacggtccgg gtggtggccg
    10441 aacgcgccgg ggcgagcggc atcacgcgga tcgtgcaagg caaggccgga ccggtgatgg
    10501 ctgccgcggg ggtcgacgcc tccaacaccg gcgggcgcga cgtcctgctc ctgctccccc
    10561 acgaccccga tgccgtatgc cgcgacctcc acgccgcgct ccgcgccgcc accggggtcg
    10621 aggtgttcgc ggtggtcctc acggataccg ccggtcgacc gtggagggag ggtcaggtcg
    10681 atttcgcgct ggggtcgcac gggctgcggg tgcttgatga tctgcgcggg tcggtcgacg
    10741 ccgacgggcg cgccctggag gtgaccgcgc gggctgtggc cgacgagatc gcggccgccg
    10801 cggatctggt caagggcaag acggccgccg ttccggcggc gttggtgcgc ggcctggcgg
    10861 cttcggtgct tccggtggtg aacgaccgtg agggagtggg gatggcccag gcgggatcct
    10921 cgccgcaggc gggagcctcg cgactggtcc gggtcggcga tggtgactgg ttcgcgctgg
    10981 gatcccagga ggccgttcgc gcggccctcg gagtgccgcc gggtcctggt tcaggaagcg
    11041 cgcacggacc tcgccccgtc gcgggggaca cgctggctcg gcgagtgatc cgagcgctgg
    11101 atgtcgcctt ccgcccccac ctggacgact ggcatggcgg agttgctgtc gcggggccgc
    11161 ccggcacggt ggccggcgag cgggtgggcg acctcgtggt gtccggctcc aacgccttca
    11221 gggtcggtgt ggtggccgcc cggctgcagg tggcgctgcg cggcgagggc gtggcgttcg
    11281 aggtcgaccc cgagccgacc gtcgacgcag ccggtgagtg gcgggccacc ctgaggatcg
    11341 gcgacggaat agtgggcgaa tcagcggggt aggtgggtcc ggggcgccat ccgggggcca
    11401 tgccttggcc gttggtggcc ggccaactcc agcag
//
LOCUS       DAOMZY010000069        11399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5321_length_11399_cov_5.719499, whole genome shotgun sequence.
ACCESSION   DAOMZY010000069 DAOMZY010000000
VERSION     DAOMZY010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 11399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11399
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5321_length_11399_cov_5.719499"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="PLK64_05600"
     CDS             complement(<1..996)
                     /locus_tag="PLK64_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79248.1"
                     /translation="MRTQIEGSLAVAQAVAACRPQVICAYPISPQTHIVEGLSAIVKR
                     GELPGCEYLNVESEFAAMSGAIGASAAGARSYTATASQGLLYMVEAVYNASGLGLPIV
                     MTVANRAIGAPINIWNDHTDSMSQRDSGWIQLYAETNQEAVDLHVQAFRIAEELSLPV
                     MVCMDGFILTHAVEEVDVPEAADVARFLPPFEPRQVLDPANPVSIGAMVGPEAFTEVR
                     YLAHERQMQALDLIPAVAAEFQAVFGRDSGGLLHTYEIEDAETVVIALGSVLGTIKDV
                     VDERRARGEKIGVLGITSFRPFPLKAVEAAIGNAKRFVCLEKAFSVGIGGIVSSHV"
     gene            complement(1007..1582)
                     /locus_tag="PLK64_05605"
     CDS             complement(1007..1582)
                     /locus_tag="PLK64_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPV79249.1"
                     /translation="MFEVRIHGRGGQGVVTAAELLSVAAFDEGRHAQAFPSFGSERTG
                     APVVAFCRVADQEIRVREPVVTPDALVVQDSTLLHQVDVFAGLKADGYALINSPLTPS
                     ELGLDELAARLGVGRLLTIPATDIAREHIGRPVPNLVMLGGLAAFTGLVHLPALLEAM
                     QDHFPGALGAKNVAAATAAYELVQSLVPSHT"
     gene            1842..3347
                     /locus_tag="PLK64_05610"
     CDS             1842..3347
                     /locus_tag="PLK64_05610"
                     /EC_number="2.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA carboxytransferase subunit 5S"
                     /protein_id="HPV79250.1"
                     /translation="MSKKIDVTELALRDAHQSLFATRMALEDMVPICEDIDQAGYWSV
                     ECWGGATFDACIRFLNEDPWERLRTFRSLLPNSKLQMLLRGQNLLGYRHYEDDVVDRF
                     VGKAVENGMDVFRTFDALNDVRNVKRAIAAVKREGAHAQGTLCYTVSELHTVEGYVQM
                     ARDLMDLGCDSLCLKDMAALLQPQPAYDIVKAIKDDLGQDVRIHIHCHATTGVTLVSL
                     MKGIEAGADCVDAAISSMSLGPGHNPTESLVEMLPGTGFHTELDEGRLIKIKDYFAGI
                     RPKYAEFASNIVGVETEIFKSQIPGGMISNMESQLKAQGAAHRIKEVLEEVPRVRADA
                     GYPPLVTPSSQIVGTQAVFNVMMGRYKVLTGEFADVMLGYYGSTLGTPNPEVVEIARA
                     QAKKDPITCRPADLLPAEWEKDREEAGALRGFNGSDEDVLTYAMFPKVAPTFFGTRDE
                     GPKNVGKDPDTLAAEKAATEAGAQPGQPVRTPIRYKVIVNGAPHSVQVQPS"
     gene            3373..4911
                     /locus_tag="PLK64_05615"
     CDS             3373..4911
                     /locus_tag="PLK64_05615"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPV79251.1"
                     /translation="MQEKIDQLHQRRASLAAAGGEQRVAKQHEQGKLTARERIEELLD
                     EHSFQESAVYAVHRANRFGMSDKDVPSDGVVVGQGTVLGRLVHVASQDFTILGGTIGE
                     LHADKIAQAANMALRTGSPFVCINDSGGARVQEGVDALNGCGHIFYANVLLSGVVPQI
                     SILAGPCAGAAAYSPALTDFIIQTKQAQMYITGPDVIKQVTGEEVSHEDLGGAAVHAG
                     RSGVVHFVAEDDAQAALIAKKLLSFLPQNNTEDPPEVEHDGRADDDPFLDTVIPEDYR
                     KPYDVRDVIARVVDDADFLEVHASWAANAVVGFGRIVGRTVGVIANQPNQLSGVLDIN
                     ASDKIARFVRFCNAFNIPLLTFVDTPGFMPGVDQEYNGIIRHGAKVLFAYSAATVPKV
                     TVVLRKAFGGAYIAMCARALGADKVFAWPSAEIAVMGAEGAAGIVFRKEISQADDPAV
                     KRQELIDEYRGTFANPYVASEHGLVDDVIVPAETRRYVAQALESLTTKRDMRPPKKHG
                     LMPM"
     gene            4956..5234
                     /locus_tag="PLK64_05620"
     CDS             4956..5234
                     /locus_tag="PLK64_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79252.1"
                     /translation="MTSTPMDVARLADLVEEMQRRITKLEKQLAEAQGPQGPVPIDTI
                     LAISAAVAAYLGKKATVRQIHYSHTNSWARMGRSNIHASHTFTHTRSH"
     gene            5258..5641
                     /locus_tag="PLK64_05625"
     CDS             5258..5641
                     /locus_tag="PLK64_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HPV79253.1"
                     /translation="MRLKVTVDGVEYDVLVDVEAEPKPALGGFVFSSTGTATMPRSGP
                     AASAGEAAGGGGGENALRAPIAGTVLQVNVQPGAEVKEGDTLIILEAMKMETEITAPV
                     AGTVKSVPVQVGSPVQGGDILVDFE"
     gene            5885..7351
                     /gene="ahcY"
                     /locus_tag="PLK64_05630"
     CDS             5885..7351
                     /gene="ahcY"
                     /locus_tag="PLK64_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009356244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPV79254.1"
                     /translation="MSFTDFKVADLSLAAAGRHQLRLAEHEMPGLMALRAQYREAQPL
                     AGARIAGSLHMTVQTAVLIETLVALGAQVRWASCNIFSTQDEAAAAVVVGPTGTPEDP
                     QGVPVFAWKGETLEDYWWCTDQILTWPADASGSTGPNMILDDGGDATMLVHKGREWEK
                     AGQVPPTTEADSEEFAVFKALVRQTIAADAGKWTRTAAGIKGVTEETTTGVHRLYQLA
                     EAGELLFPAINVNDSVTKSKFDNKYGCRHSLVDGLNRATDVLIGGKVAVVCGFGDVGK
                     GCAQSLAGQGARVIVTEVDPICALQAAMEGYQVARLEDVIGSADIVITATGCFDVVTA
                     AHMAAMKDKAIVANIGHFDNEIDMAGLARTPGIVKTEIKGQVHEWTFPASADRPEHSV
                     IVLSEGRLMNLGNATGHPSFVMSNSFSNQTIAQIELFTKTDDYELQVYTLPKVLDELV
                     ARLHLDALGVALTELTKEQAEYLGVDVAGPFKPDHYRY"
     gene            complement(7421..8902)
                     /locus_tag="PLK64_05635"
     CDS             complement(7421..8902)
                     /locus_tag="PLK64_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79255.1"
                     /translation="MTLGADLQTGVQERQPRMLQWLPLAALVALPLLLIREGARPITD
                     PDAAWHVLLGKAVLSGVSLGTDVDPLTTRTSVPWVFHQWLPEVAAAIADERLGWWGVG
                     WLAHLGRLFVMLALWWVCRREASRLAAVVALAIAIVGTSMSLGPRPQLIGLALLAVSI
                     GAWRSSAQDGRPRWWLVPLTWVWACCHGTWVLGLAVGVVSVVAIGIDRWRAQSAGAGP
                     TSAAPWREVWRSIRRLGLVPVLSGLAVLVTPVGPRLLTAQQHIAAVSDLVTEWQRPSL
                     ADTPVALTWAAAAVVILSMTFGRDRRSLSAVDLATSGMALVSLLAYQRTVAVAAVLLA
                     PLLAARLNAWLGRARPATDGADRWLLPITAVVSALVMGVLLPRVAAQPGGVPTNLVPA
                     LSALPAGTVVLDDERLGGWLLATQPSLTPTVDSRFEAYGRAYLQQQLDLVVGAPGWRE
                     RLAATGASAVVLPTQAPLIDLLSADPSWRHVGQDVGYTLFVRR"
     gene            8987..10048
                     /locus_tag="PLK64_05640"
     CDS             8987..10048
                     /locus_tag="PLK64_05640"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPV79256.1"
                     /translation="MTTAAQATQGRTTGRVGALDGLRGVAVLGVTWCHLALHYGLTPR
                     APGGFIGVVVFFVLSGYLITGIVWRGASFPTGYGTFIRRRATRLGPAIAGMCAVYLVV
                     MPSLGDRELVSTLRYAGLALVQGTGWDYSSEQSQVPPEFAPTWSLTVEWTFYVLWPLV
                     LLVLRAWRLDPRGVLRLAVAAAVVLAGLGLLLPDRALYVLPVANLSVMLLGAALALSE
                     VDRSSRGRPPVQVDPGFALLALLAIVTLSVIPGYPMGPAYRYAVMPATTGFTLLVIAS
                     IRDQGSIVRRMLGAPVLRAVGERAYSLYLWQVGVFWLVWRLLGSDRPWVAAMAAVLVL
                     SVTVELSFRLLEKPVLHQP"
     assembly_gap    10057..10156
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10233..10910
                     /gene="mtrA"
                     /locus_tag="PLK64_05645"
     CDS             10233..10910
                     /gene="mtrA"
                     /locus_tag="PLK64_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrAB system response regulator MtrA"
                     /protein_id="HPV79257.1"
                     /translation="MKGRVLIVDDDLALSEMLSIVLRQEGLDVTHVADGTSAVAAFRD
                     SRPDLVLLDVMLPGMDGMEVCRRIRAESGVPIVMLTARTDTVDIVVGLESGADDYVVK
                     PFKPQELVARIRARLRRGDEPEPERLDIGDLQIDIAGHSVKREGRPLSLTPLEFDLLV
                     ALARKPWQVFTREVLLEQVWGYRHAGDTRLVNVHVQRLRSKIEHDPERPQIVVTVRGV
                     GYKAGPA"
     gene            11123..>11399
                     /locus_tag="PLK64_05650"
     CDS             11123..>11399
                     /locus_tag="PLK64_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79258.1"
                     /translation="MDRDAGAELDKRFATSEADSVGSDSQARLWPEPGEIKTDLPLAP
                     AFDAKTLLPATLADFVLDEADRMPCSPDYIAAALIVCLGSVIGARCAI"
BASE COUNT         1892 a         3879 c         3799 g         1729 t
ORIGIN      
        1 cacgtgcgag gagacgatgc cgccgatgcc caccgagaag gccttctcga ggcagacgaa
       61 gcgcttggcg ttgccgatcg cggcctcgac ggccttgagc gggaacggcc ggaacgaggt
      121 gatcccgagg acgccgatct tctcgcctcg ggcccgtcgc tcgtcgacga cgtccttgat
      181 cgtgcccagc accgacccga gcgcgatgac gacggtctcg gcgtcctcga tctcgtaggt
      241 gtgcagcagc ccgccggagt cgcggccgaa gaccgcctgg aactcggcag ccaccgcggg
      301 gatgaggtca agggcctgca tctggcgctc gtgggcgagg tagcggacct cggtgaacgc
      361 ctcggggccg accatcgcac cgatcgacac ggggttggcc gggtcgagca cctggcgcgg
      421 ctcgaacggc ggcaggaacc gcgcgacgtc ggcggcctcg ggcacgtcga cctcttcgac
      481 cgcgtgggtg aggatgaagc cgtccatgca gaccatgacg ggcagggaca gttcttcggc
      541 gatccggaag gcctgcacgt gcaggtcgac cgcctcctgg ttggtctcgg catacagctg
      601 gatccagccg gagtcgcgct gactcatcga gtcggtgtgg tcgttccaga tgttgatcgg
      661 cgcgccgatg gcccggttgg cgaccgtcat gacgatcggc aacccgagcc cgctggcgtt
      721 gtagacggcc tccaccatgt agagcaggcc ctggctggcg gtggccgtgt aggaccgcgc
      781 accggctgcc gaagccccga tggcccccga catcgcagcg aactcgcttt cgacgttgag
      841 gtattcgcag ccgggcaact cgccacgctt gacgatggcc gagaggcctt cgacgatgtg
      901 cgtctggggc gagatggggt aggcacagat gacctgcggc cgacacgcgg cgaccgcctg
      961 ggcaaccgcg agcgagccct cgatctgggt gcgcaacgga cgtctcctag gtgtgggacg
     1021 gaacgaggga ctgaacgagc tcgtatgccg cggtcgcggc tgccacgttc ttggcaccga
     1081 gggcgccggg gaagtgatcc tgcatcgcct caaggagcgc gggcaggtgg accaggccgg
     1141 tgaacgcggc caggcctccg agcatcacga ggttgggcac cggtcgcccg atgtgctcgc
     1201 gtgcgatgtc ggtcgccgga atggtgagga ggcggccgac tccgagtcgg gccgccagct
     1261 catccaggcc gagttcgctc ggcgtgaggg gcgagttgat gagtgcgtag ccgtccgcct
     1321 tgaggccggc gaagacgtcg acctggtgca gcagggtcga gtcctgcacc acgagggcgt
     1381 cgggagtcac gacgggctcc cggacgcgga tctcctggtc ggcgacgcga cagaacgcga
     1441 cgacaggggc tccggtgcgc tcggaaccga aactggggaa ggcctgggca tggcgacctt
     1501 catcgaatgc ggcaacggac agcaactcgg cggcagtgac cacgccctgt ccgcctcggc
     1561 cgtggatgcg gacctcgaac atgcgttgaa cgtatgccgc ggatcacgat gaggcctagg
     1621 gaccaatggt cctcacttcg accgccatgc tcagccgcag gtgaacatcg gatggacacc
     1681 tgccgcgggg cggcataccg catggggaag gtgaccaaag ccgggccaga ggtcccagga
     1741 tcgagtccgt tatgcgtgcc acattgacgg gcaacgaggg tatgccgcgt cccgcaatcc
     1801 gcggctcccg ttgactgccg ccgtcggaag gtgtcgccac gatgagcaag aagatcgacg
     1861 tcacggagct cgccctgcgc gatgcccacc agagcctgtt cgccacccgg atggccttgg
     1921 aagacatggt cccgatctgc gaggacatcg accaagccgg gtactggagc gtggagtgct
     1981 ggggcggcgc gaccttcgac gcctgcatcc ggttcctcaa cgaagacccg tgggagcggc
     2041 tgcgcacttt ccgcagcctg ctgcccaact ccaagctgca gatgctcctg cgcgggcaga
     2101 acctcctcgg ctaccgccac tacgaggacg acgtcgtcga ccgcttcgtc ggcaaggccg
     2161 tcgagaacgg catggacgtc ttccgcacgt tcgacgccct caacgacgtg cgcaacgtca
     2221 agcgcgccat cgctgcggtc aagcgtgaag gggcgcatgc ccagggcact ctctgctaca
     2281 ccgtgagcga gttgcacacg gtcgagggct acgtccagat ggcccgtgac ctcatggatc
     2341 tcggatgcga ctcgctgtgc ctcaaggaca tggcggcgct gctgcagccg caaccggcat
     2401 acgacatcgt caaggcgatc aaggacgacc tcgggcagga cgtccgcatc cacatccact
     2461 gccacgcgac gaccggcgtc accctcgtgt cgttgatgaa gggcatcgag gccggcgccg
     2521 actgcgtcga tgccgcgatc tcctcgatga gcctcggccc gggtcacaac cccaccgaga
     2581 gcctcgtcga gatgctgccc ggcaccgggt tccacaccga gctcgacgag ggtcgactca
     2641 tcaagatcaa ggactacttc gccgggatcc gcccgaagta cgccgagttc gcgtcgaaca
     2701 tcgtcggtgt cgagaccgag atcttcaaga gccagatccc cggcgggatg atctccaaca
     2761 tggagagcca gctcaaggct caaggggctg cccaccggat caaggaggtg ctcgaggagg
     2821 ttccgcgggt gcgcgccgac gccggctacc cgcccctggt caccccgtcg agccagatcg
     2881 tcggcaccca ggcggtcttc aacgtcatga tgggccgcta caaggtcctc accggtgagt
     2941 tcgccgacgt catgctcggc tactacggca gcacgctcgg cacgccgaac cccgaggtcg
     3001 tcgagatcgc ccgggcgcag gccaagaagg acccgatcac ctgccggccg gctgacctgc
     3061 tcccggccga gtgggagaag gaccgcgagg aggccggcgc gctgcgcggc ttcaacggtt
     3121 ccgacgagga cgtgctcacc tacgcgatgt tcccgaaggt cgctccgacc ttcttcggca
     3181 cccgtgacga gggccccaag aacgtcggca aggaccccga cacgctcgcc gccgagaagg
     3241 ctgccaccga ggccggcgcc cagcccggcc agccggttcg tacccccatc cgctacaagg
     3301 tcatcgtcaa cggcgccccc cacagcgtcc aagtccagcc gtcctgaaag gtcagccgtg
     3361 gcagcgcaga gcatgcagga gaagatcgac caactgcacc agcgtcgggc atcgctcgcc
     3421 gccgccggag gcgagcagcg ggtcgccaag cagcacgagc agggcaagct caccgcccgt
     3481 gagcggatcg aggagctcct ggacgagcac tcgttccagg agtcggcggt gtatgccgtg
     3541 caccgcgcca accgcttcgg catgtccgac aaggacgtgc cgagcgacgg cgtcgtcgtc
     3601 ggtcagggca cggtgctcgg gcggctcgtc catgtggcca gccaggactt caccatcctg
     3661 ggcggaacca tcggtgagct gcacgccgac aagatcgcgc aggccgccaa catggcgttg
     3721 cgcaccgggt cgccgttcgt gtgcatcaac gactcgggtg gtgcgcgggt ccaggaaggt
     3781 gtcgacgcgc tcaacggctg tggccacatc ttctacgcca acgtgctgct ctcgggtgtg
     3841 gtcccgcaga tctcgatcct ggccggcccc tgcgccgggg ccgcggcata ctcgccggct
     3901 ttgacggact tcatcatcca gaccaagcaa gcgcagatgt acatcaccgg ccctgacgtc
     3961 atcaagcagg tcaccggtga ggaggtcagc cacgaggacc tcggcggcgc ggcggtccac
     4021 gccggccgct cgggggtcgt tcacttcgtc gccgaggacg acgctcaggc ggcgttgatc
     4081 gccaagaagc tcctgagctt cctcccgcag aacaacaccg aggacccgcc cgaggttgag
     4141 cacgacggca gggccgacga cgacccgttc ctcgacaccg tgatcccgga ggactaccgc
     4201 aagccctacg acgtgcgtga cgtcatcgcc cgcgtcgtcg acgacgcgga cttccttgag
     4261 gttcacgcca gctgggccgc caacgccgtc gtcggcttcg gccggatcgt tgggcgcacg
     4321 gtcggcgtca tcgccaacca gcccaaccag ctctccggtg tcctggacat caacgcgtcc
     4381 gacaagatcg ctcgcttcgt gcggttctgc aacgccttca acatcccgct gctcaccttc
     4441 gtcgacaccc ccggcttcat gcctggcgtc gatcaggaat acaacgggat catccggcac
     4501 ggcgccaagg tgttgttcgc ctactcggcg gccaccgttc cgaaggtgac ggtcgtcctg
     4561 cgcaaggcct tcggcggtgc ctacatcgcc atgtgcgcgc gagcgctggg tgccgacaag
     4621 gtgttcgcct ggccgtcggc cgagatcgcc gtcatgggcg ccgaaggtgc cgcgggcatc
     4681 gtcttccgca aggagatcag ccaggcggac gacccggccg tcaagcgcca agaactcatc
     4741 gacgaatacc gcggcacctt cgccaacccg tatgtggcga gcgagcacgg tctcgtcgac
     4801 gacgtcatcg tgccggccga gacccgccgc tacgtggccc aggccttgga gtccttgacg
     4861 accaagcgcg acatgcgtcc gcccaagaag cacggcctca tgccgatgtg acctggcgac
     4921 cgacgacgac gaacagataa ccacgagagg ttcttatgac aagcaccccg atggatgtcg
     4981 ctcgcttggc cgacctggtc gaggagatgc agcgacggat caccaagctg gagaagcagc
     5041 tggccgaggc gcaggggccc caggggccgg tgccgatcga cacgatcctc gccatctccg
     5101 ccgcggtcgc ggcctacctg ggcaagaagg cgaccgttcg ccagatccac tacagccaca
     5161 ccaactcctg ggcacggatg ggccgctcca acatccacgc ctcgcacacc ttcacccaca
     5221 cccggtccca ctgacccgcg actgacaagg aacgcccatg cgactcaagg tgaccgttga
     5281 cggggtcgaa tacgacgtgc tcgtcgacgt cgaggccgag ccgaagcccg cgttgggcgg
     5341 cttcgtcttc tcctccaccg gcacggcgac gatgccgcgc tccgggccgg cggcatctgc
     5401 cggtgaggcg gctgggggtg gcggcggcga gaatgcactg cgggcgccga tcgccggcac
     5461 cgtgctgcag gtcaacgtcc agccgggcgc cgaggtcaag gagggcgaca ccctcatcat
     5521 cctcgaggcc atgaagatgg agacggagat caccgcgccc gtggcgggca cagtgaagtc
     5581 ggtcccggtc caggtcggtt cgcccgtgca gggcggggac atcctcgtcg acttcgagta
     5641 gcctcaccag cccaccccac ccacccgcgc ggcccggtcc gcgccgagag tatgccgccc
     5701 gctcaccgca gcgggcggca tacgtgtgct cgggtaggat gcccagacgt gccggcgtgg
     5761 gtggaggacc cgcgtgcgga ttgccgccag aggtgcctgt cctgctggac aggaccacac
     5821 ggcagaccgc tgccctgaag ggcgacgccg ggtctgccca ccgaccatcc cagggagccc
     5881 tgccatgtcc ttcaccgatt tcaaggtcgc cgacctcagc cttgcggcgg ccggccgcca
     5941 ccaactgcgc ctcgccgagc acgagatgcc cggcctgatg gcgctgcgcg cgcagtaccg
     6001 agaggcccag ccgctggccg gagcccggat cgccgggtcg ctgcacatga ccgtgcagac
     6061 ggccgtgctc atcgagaccc tcgtcgcgct cggcgcgcag gtccgatggg catcgtgcaa
     6121 catcttctcg acccaggacg aggccgccgc ggccgtcgtc gtcggtccga ccggcacccc
     6181 tgaggacccc cagggtgtgc ccgtcttcgc ctggaagggc gagacgttgg aagactactg
     6241 gtggtgcacc gatcagatcc tcacctggcc cgccgacgcc tccggctcga ccgggccgaa
     6301 catgatcctc gacgatggcg gcgacgcgac catgctcgtc cacaagggcc gcgagtggga
     6361 gaaggccggt caggtgcccc cgacgaccga agccgactcg gaggaattcg ccgtcttcaa
     6421 ggcgctggtg cgccagacca tcgccgccga cgcgggcaag tggacccgca ccgcggccgg
     6481 catcaagggg gtcaccgagg agacgaccac cggtgtccac cggctctacc agctcgcgga
     6541 ggcgggcgag ctgctcttcc cggcgatcaa cgtcaacgac tcggtgacca agagcaagtt
     6601 cgacaacaag tacggttgcc gccactccct cgtcgacggc ctcaaccggg ccaccgacgt
     6661 cctcatcggc ggcaaggtcg ccgtggtctg tggcttcggc gacgtcggca agggctgcgc
     6721 ccagtcgctg gccggacagg gtgctcgggt catcgtcacc gaggtcgacc cgatctgtgc
     6781 gctgcaggca gccatggagg gctaccaggt ggcccggctc gaggacgtca ttggctcggc
     6841 cgacatcgtc atcactgcca cgggctgctt cgacgtcgtc accgccgctc acatggcggc
     6901 gatgaaggac aaggcgatcg tcgccaacat cggtcacttc gacaatgaga tcgacatggc
     6961 ggggctagcc cgcacacccg gcatcgtcaa gaccgagatc aaggggcagg tccacgagtg
     7021 gacgttcccc gcgtcggccg accgtcccga gcactcggtc atcgtgctct ccgagggccg
     7081 gctgatgaac ctcggcaacg ccaccggcca cccgagcttc gtcatgagca actcgttcag
     7141 caaccagacg atcgcccaga tcgagctctt caccaagacc gacgactacg agctgcaggt
     7201 ctacacactg cccaaggtgc tcgacgaact cgtcgcccgg ctgcacctgg acgcgctcgg
     7261 ggtggccctc accgagttga ccaaggagca ggcggagtat ctcggggtgg acgtggccgg
     7321 cccgttcaag cccgaccact accgctactg atcaccgcgc ctgcgctccg gcaacgccgg
     7381 cagctggcgc cgggtcaacg tgccacgcta tgccgtctgg tcacctgcgc acgaaaagcg
     7441 tgtagccgac gtcctgtccg acgtgccgcc aggaggggtc ggcggacaaa aggtcgatga
     7501 ggggcgcctg ggtcggcagg actacagcac tggctccggt tgctgccagg cgctcccgcc
     7561 atccaggcgc cccaaccacc agatccaact gctgctgcag gtaggcccgt ccgtaggcct
     7621 cgaatcggga gtcgactgtc ggcgtgaggg agggttgcgt cgcgagcagc cagccgccca
     7681 gccgttcgtc gtccaggacg acagtccctg cgggcaatgc ggagagcgca gggaccaggt
     7741 tggttgggac gccaccgggc tgggcggcca cccggggtag tagcactccc atgacgaggg
     7801 cgctgacgac agcagtgatg ggcaggagcc acctgtccgc accgtcggtg gcggggcgag
     7861 cgcgaccgag ccaggcgttc aaccgggcgg ctagcagagg ggccagcaaa acggccgcga
     7921 cggccaccgt gcgttggtac gccaggagac tgaccagggc catcccgctg gtcgccaggt
     7981 caacggccga cagcgaccgg cgatcacggc caaacgtcat cgacaggatc acgaccgccg
     8041 ctgccgccca cgtgagcgcg acgggtgtgt ccgccaggct gggtcgctgc cattcagtca
     8101 ccaggtcgct gacggcggcg atgtgttgct gagcggtgag caggcgaggc cccaccggtg
     8161 tcaccaggac cgccagcccg ctgagcacag gcaccaggcc cagccgccgg atcgatctcc
     8221 agacctctcg ccagggcgct gcggacgttg gccccgcccc ggccgactgg gcccgccacc
     8281 gatcaatccc gatcgcgacg accgacacga cgccaacggc gagtcccaat acccaggtgc
     8341 catgacagca ggcccacacc caggtcagcg gcacgagcca ccaacgaggt cggccgtcct
     8401 gggctgagct gcgccatgcc ccgatgctga cggccaacag agccaagccg atcagctgtg
     8461 gacgcggtcc gagtgacatc gacgtcccga cgatggcgat ggcgagcgcg acgacggccg
     8521 ccaagcgaga ggcttcgcgg cggcataccc accacagtgc cagcatgacg aagaggcgac
     8581 cgagatgcgc gagccaaccc acgccccacc aaccgaggcg ctcgtcggcg atggccgcgg
     8641 ccacctcggg gagccactgg tggaacaccc atggcacgct tgtgcgcgtt gtcaacgggt
     8701 cgacatcggt gccgaggctg acccccgaca ggaccgcttt gccgagcagg acgtgccacg
     8761 ccgcatccgg atcggtgatc gggcgggcgc cttcgcggat gagcaacagc ggcagcgcta
     8821 cgagcgccgc cagcggcagc cactgcagca tccgcggctg gcgttcctgc acgccggttt
     8881 ggaggtccgc ccccagagtc acgcgaccag tatgtccggg aggggcgaac ggcgcttgac
     8941 ccgttcggcg gatgccactg acggcgcctc gggggacaat cgccacgtga cgacggcggc
     9001 tcaggcgacg caagggcgga ccaccggccg cgtaggcgcc ctcgatggcc tgcgtggggt
     9061 ggccgtcctc ggcgtcacgt ggtgtcacct cgcgcttcac tacggactca cgccacgcgc
     9121 accgggcggt ttcattggcg tggtcgtctt cttcgtcctg tccggctacc tcatcacggg
     9181 gatcgtgtgg cgaggcgcca gcttcccgac cggctatggg acgttcatcc gccgccgggc
     9241 gacccgcctc ggaccggcca ttgcggggat gtgcgctgtc tacctcgtcg tcatgccgtc
     9301 gctcggcgat cgcgagctgg tcagcacgct gcggtatgcc gggctcgcgc ttgttcaggg
     9361 gacgggctgg gactattcgt ccgagcagtc ccaggtccct ccggaattcg cgccgacgtg
     9421 gagcttgaca gtcgagtgga ccttctatgt gctctggccg ctcgtcctcc tcgtcctgcg
     9481 tgcctggcgc ctggacccgc gcggtgtgct gcggctcgca gtcgccgctg ccgtcgtcct
     9541 ggctggcctg gggctgctcc ttcctgaccg ggcgctctat gtgttaccgg tcgcaaacct
     9601 cagcgtcatg ttgcttggag ccgcgctggc cctcagcgag gttgatcggt ccagcagggg
     9661 gcgtcctccc gtgcaggtgg accccgggtt tgcgctgctg gcgctgctgg ccatcgtgac
     9721 cctcagcgtc attcccggct atcccatggg tccggcctac cgctatgccg tcatgccggc
     9781 gaccaccggg ttcacgctgc tggtgatcgc ctcgattcgc gaccaggggt cgatcgtgcg
     9841 gcggatgctg ggcgccccgg tcctgcgggc agtgggtgag cgcgcctaca gcctctacct
     9901 ctggcaggtc ggcgtcttct ggctggtctg gcgtctgctc ggttccgacc gtccatgggt
     9961 cgccgcaatg gccgccgttc tagtcttgag cgtcaccgtc gagttgagtt tccggctgct
    10021 ggagaagcct gtgcttcatc agccctgagg cgtggtnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nnnnnnaggc taggcccgac ctgactcatc cacgatgtgt gatgattctg
    10201 gacagtcggg ggaactgtgg aagggaactc acatgaaggg ccgcgtgctg atcgtcgatg
    10261 acgacctcgc cctctcggag atgctgagca tcgtcctgcg ccaggaaggg ctggacgtca
    10321 cccacgtggc cgacggcacg tccgccgtcg cggccttccg cgactcacgt ccggacctcg
    10381 tgttgctgga cgtcatgctt cccggcatgg atggcatgga ggtatgccgt cgcatccgcg
    10441 ccgagtccgg cgtccccatc gtcatgctca ccgctcgcac cgacaccgtt gacatcgtcg
    10501 tcgggctgga gtctggggcg gacgactacg tcgtcaagcc cttcaagccg caggagctcg
    10561 tcgcgcgcat ccgggcccgg ctgcgtcggg gcgatgagcc ggagccggag cgcctggata
    10621 tcggcgacct gcagatcgac atcgctggac actcggtcaa acgtgagggt cggccgctgt
    10681 cgctgacccc gctggagttc gacctgctgg tcgccctcgc ccgcaagccc tggcaggtgt
    10741 tcacccgcga ggtgctcctg gagcaggtgt ggggctaccg ccacgcgggg gacacccggc
    10801 tggtcaacgt ccacgtccag cgactgcgca gcaagatcga gcacgacccc gagcgtcccc
    10861 agatcgtcgt gacggtccgc ggggtcggct acaaggccgg cccggcctga gcggatgacc
    10921 gactcgagcc atcgccttgg atcggcggga ccggccggcc cggctggacc ggctggacgg
    10981 gcgccgcgct ggccctgctc gtggccaacc tggcctcgtt cgcactgttc gccgtggaca
    11041 agtcgcgggc gcgccgcggt gcgcgccgga tccccgagcg catcctgctg tggtcggccg
    11101 cgctgtccgg gaccatcggg gcgtggatcg cgatgccggt gctgaactgg acaagcgatt
    11161 tgctacctca gaagcggatt cggtaggttc agacagtcaa gcccgcttgt ggccagagcc
    11221 aggtgagatc aagactgatc ttcctcttgc gccagcattc gacgccaaga ctttgctgcc
    11281 agccacgttg gctgactttg tgctggacga ggcagaccgc atgccctgtt cgccggatta
    11341 cattgcggcg gcgctgatcg tctgcctggg ctcggtcatc ggtgcgaggt gcgccatca
//
LOCUS       DAOMZY010000070        11388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5331_length_11388_cov_5.527751, whole genome shotgun sequence.
ACCESSION   DAOMZY010000070 DAOMZY010000000
VERSION     DAOMZY010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 11388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11388
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5331_length_11388_cov_5.527751"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PLK64_05655"
     CDS             <1..769
                     /locus_tag="PLK64_05655"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPV79259.1"
                     /translation="TATQTTLRAILAGLPTDPSVPLPPGTPTRAELTAQLAAVATGIA
                     TATAGLAKLDAGLTEMRSGLKQIDAGLAQLATKASEVRTGLSTVEDGRAALADARVQL
                     VDAQAISRVMADTAAVAVDLAREQQARTTVTAPVTGVVVKAASAGDLLPPGAVLATIR
                     PTGPATIQVWLAPDQLATICLDDPASVRGDWMPAGAVVPGAVTLISDRADYPPSSNVT
                     DEVHLTRAVPVDVTTQNPLPAGVPVEVTLRGCHPHTK"
     gene            811..1596
                     /locus_tag="PLK64_05660"
     CDS             811..1596
                     /locus_tag="PLK64_05660"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPV79260.1"
                     /translation="MAGHGRKHPPIPVIVVLVVALAGGGWWWWRTANQPEASASTALN
                     GQVEARQYDISSAIAGRVLTVPVAEGDQVTKGQPIVTLDTAALDLQLKQAQQGVIAAQ
                     AAVTNAKDSGTKADVTAAEARVEQANAAVSLAQVQLGYASVSAPRDGRVVTVVTNVGQ
                     NAAPGRILASIVDLHDLFVRVYVPETRIGTVSVGQAVSVSSDSGGAAIAGTVSFVSAQ
                     AEFTPNTVQTAEQRAKLVYEVRVTISDSSGTLKPGMPVDVTLR"
     gene            1598..2548
                     /locus_tag="PLK64_05665"
     CDS             1598..2548
                     /locus_tag="PLK64_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV79261.1"
                     /translation="MAITGASPAAPAVSTQGLTRRFGDVVAVDGVDLVVQRGAVFGLL
                     GPDGAGKSTLIRMLATVLLPDAGEALVLGHSTASEASAITGRIGYMSQRFSMYPDLTV
                     AENLDFFATVRGVPKQVRRERSAALLDSMGLAEFGGRQARHLSGGMKQKLMLATTLMH
                     EPELLLLDEPTTGVDPVSRREFWRVLAGLHRGGTTVVVATPYMDEAERCSAIAFLQDG
                     RIQQQGTPEEITALVPGTLVEVSGADPRAALAALADLPGVGSPHVFGEVVRVLWEGSL
                     ASVEGAITTHLRAAGFPTVRVTPTRVDMETAFAVLAEAGR"
     gene            2545..3684
                     /locus_tag="PLK64_05670"
     CDS             2545..3684
                     /locus_tag="PLK64_05670"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV79262.1"
                     /translation="MSGAGVRIGAVVRKEFIHVGRDPRLLAVVLIMPIIQLLLFAYAI
                     SFDVRNLPTVVVDLDHTPDSAAYLRAYEASGLFDVVGHGADPTAVDTAFDRNEAQVAV
                     IVPAGFAASLARGEQAQVGVLVDGSEPNSARVAQTYSMALNQLYGRALAVEWASTQGV
                     DLGQIGLVEPRLRTWYNPDRASSIFLVPGLMVVIIMIVTAQQTAVTLVREREQGTAEQ
                     LMVSPLRRIELMIGKLTPWVIMAFLDIVVIAVLGVVLFGVPMRGDPVALAVGATLFVA
                     SCLGIGLIVSAVAPSLDTANMAAFLVAFLPAFLLSGFAFALDQIPPVLEWISRLFPAR
                     YMVTISRSVLLKGAGFPEVWPQVAALAAYALISLLIAVRLHGRRA"
     gene            3681..4802
                     /locus_tag="PLK64_05675"
     CDS             3681..4802
                     /locus_tag="PLK64_05675"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV79263.1"
                     /translation="MSLTRIRLLVWKEFLQLRRDPLLLRLLLLMPIIQLVGFGYVVSV
                     DVRNLPTAVVDLDQTVVSRQLESSFTASGYFLVVARPDAETQLTSLLDTGVAKVALVI
                     PQGTQDLLARGQTAPVGIVVDGSDSQIASVGSGYAAQAIARFNASRTAAFTGSLQAPG
                     IDARTRVVFNPTLDPINTMIPGLVAAILMISLMSIMSQAVVRERESGTLEQMFVTPIR
                     PSEYLIGKVAPYALLAVAQLSVVAAIGMLWFKVPFNGSVWVVFVGLALFLLISVGLGL
                     LVSLISRTRQQAQQALVFIMIPTMVLSGFIFPIESMPEAIQPLTYAVPLRYALTVLRG
                     SFVKGSGFEALATPLWAMVGFAIVIFGAAVIATRRRIAE"
     gene            4815..5789
                     /locus_tag="PLK64_05680"
     CDS             4815..5789
                     /locus_tag="PLK64_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV79264.1"
                     /translation="MTREGEVHGIPADPSAAISVRGLTKTFGDFTAVDGIDLDVPRGQ
                     VFGFLGPNGSGKTTTIRILTGTSAPRSGQALVLGEDVVAHPERVRPRIGYMSQKFALF
                     EDLTVDENLRFYAGVYALDPAVFAARRRYILEMADLQGRERELTRNLSVGWRQRLALG
                     AATIHEPDLLFLDEPTSGVDPVARRQFWDLLYELAGRGVTLFVTTHYMEEASHCHRLA
                     FIYRGRIVAEGSPNEIRANLSTQRVYDVTVRDADLALGWLETADGIAEAYLSGAALHA
                     VIDRGVDRGAGALRERMVAAGFADAVVVSVEPTIEDVFVNLVSREREA"
     gene            complement(5856..>6197)
                     /locus_tag="PLK64_05685"
     CDS             complement(5856..>6197)
                     /locus_tag="PLK64_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HPV79265.1"
                     /translation="PARVTVAMTALTTWSLTGARQVYAVVRRDAPAHPSPNAGPVEAA
                     FAAALGRQLGGTNDYAGVREERGRLGDGPPVSTGDIARATALQQRIGMASLVGLVSAR
                     LLLRAVVRLRH"
     assembly_gap    6198..6297
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6298..6665)
                     /locus_tag="PLK64_05690"
     CDS             complement(<6298..6665)
                     /locus_tag="PLK64_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HPV79266.1"
                     /translation="MSLDDVAVGLVLGYAADLAFGDPRRWHPVAGFGRVAEALERHTY
                     APRRSSGIVHEAVLVGGAVALGQALTRLPRAARVPVVAAATWAVLGGRSLAREAEAMD
                     ALLQADDLAGARLRIRNLVGR"
     gene            complement(6710..7744)
                     /gene="cobC"
                     /locus_tag="PLK64_05695"
     CDS             complement(6710..7744)
                     /gene="cobC"
                     /locus_tag="PLK64_05695"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2231c family pyridoxal phosphate-dependent
                     protein CobC"
                     /protein_id="HPV79267.1"
                     /translation="MTERLDHHGDAEVRGATHLVDLAVNVRVGTPPAWLATVIRDTTG
                     QLAAYPDPSVARAALARRHAVPEDMVLPTSGGAEAFTLIARAIAARRPLVVHPQFTEP
                     EAALRRAGRVPDRHVLQAKSGFTLDPAAVDPRADLVLVGNPTNPTGVLHPRATLEALR
                     ARGRVLVVDEAFADAIRGERESLISPDLAGVLVLRSLTKTWGVAGLRAGYVVGDPDLV
                     AALAEQQSPWSVSTPAAAVMAATATPEALAEVARVAEAIARDRGHLVQALESIGLRPV
                     PGEAPFVLVEVGPGIHAELREQGYAVRRGDTFPGLDGGWVRIAVRDAATSDALVRALA
                     EVRLRGGLRR"
     assembly_gap    7865..7964
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7965..9550
                     /locus_tag="PLK64_05700"
     CDS             <7965..9550
                     /locus_tag="PLK64_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79268.1"
                     /translation="DPDCALAHWGIAFALGPNYNKPWEFFAPDEATAVLERARDEARV
                     AADCASGGTAVEQALTGALLARYPDGRPIEECTAWEVEYAAAMGQVYAAHGGDLDVAC
                     FYAEALMNLTAWQLWDQRTGEPAEGSRTLEAKAVLEAALAAPGGSQHPGLLHMYIHLM
                     EMSPTPERALPVADLLRGLVPDAGHLQHMPTHLDVLCGDYRGVVASNSAAIAADAAFV
                     AQRGPLNFYTLYRAHNLHFKIYGALFLGQERTALATVDQLEAAIPEELLRVQLPPMAD
                     WLEGFVPMRVHALVRFGRWDALIGLPLPADQDLYCTTTAMLHYGKGVAFSATGRVAEA
                     EEERRLFLAAVERVPESRMLFNNTCRDILAVASEMLDGELEYRKGNHDSAFAHLRRSV
                     ELDDGLPYDEPWGWMQPTRHALGALLLEQGHVGEAEAVYRADLGLDATLPRACQHPGN
                     VWALHGYHECLVRLAKHDLAEVVAQQLKLAGARADEPITASCLCRIDRSGALLAVDTC
                     CSGEPPSGPAEPATGCCSH"
     gene            9599..10069
                     /locus_tag="PLK64_05705"
     CDS             9599..10069
                     /locus_tag="PLK64_05705"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPV79269.1"
                     /translation="MEMTEPVRAAVQSAVLCWLATADADGRPSVSPKEIFAVVGDDIV
                     IAHIASPRSVRNIAGNPQVCLSVLDVFEQRGYRIAGRASVVTPDHDAFPTVVAPLREL
                     AGAAFPIKAVIRVAVQDVERLSAPSLWMYPDVDPAQRRAGVLEAYGVVDAPSPG"
     gene            complement(10458..>11388)
                     /locus_tag="PLK64_05710"
     CDS             complement(10458..>11388)
                     /locus_tag="PLK64_05710"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HPV79270.1"
                     /translation="LAATAASSSLEVLLAVRTILGIAGAGAFVLGGAITSTISRRHTP
                     HRAAVLIGTYFAGGGVGIVASGLLVPAVFERLGPAGWPTGWLVLAILAGVGTVAATIA
                     AYAAPTTPAAAPGSRSFWVSGLGRLASGYLLFGAGYIGYMTFIIAMLTQVGLSPKEIA
                     AFWVALGVAGAVSGQVWARLLRGARGGSAAAVLFVLLAVATGIPALTSATPALYASGV
                     LFGLCFLSLVGAVTAAGRDAVDPADTTAALGMLTTVFALGQVIGPWFTGLLADRTGGQ
                     QWGLGVSAVVLLIGAVLCRLQPPRRIGHDAGGR"
BASE COUNT         1622 a         3874 c         3955 g         1737 t
ORIGIN      
        1 gaccgccacc caaaccacgc tgcgcgccat cctcgcgggc ctgccgaccg acccaagcgt
       61 cccgctgcct ccgggcacgc ccacgagggc cgagctcacc gcgcaactcg ccgctgtcgc
      121 caccgggatc gccaccgcca ccgccggcct ggcgaaactc gacgccggcc tcaccgagat
      181 gcgctcgggc ctcaagcaga tcgacgccgg gctggcccaa ctcgccacga aggcgagcga
      241 ggtccgcacc ggactgtcga ctgtcgagga cggccgcgcc gccctcgccg atgcccgggt
      301 gcaactcgtc gacgcccagg ccatcagccg ggtcatggcc gacaccgctg cggttgccgt
      361 cgatctggcc cgagagcagc aggcgcggac gaccgtcacc gctccggtga ccggtgtggt
      421 cgtcaaggcc gcctccgcag gcgacctgct gccacctggg gccgtcctcg cgacgatccg
      481 gcccaccggg ccggcgacca tccaggtctg gctcgcgccg gaccaactcg ccaccatctg
      541 cctggacgac cccgcttcgg tgcgcggcga ctggatgccc gccggcgccg tcgtgccagg
      601 cgcggtcacc ctcatcagcg accgcgccga ctacccaccg agctccaacg tcaccgacga
      661 ggtgcacctc acgcgggccg tgcccgtcga cgtgacgacc cagaacccgc tgcccgcggg
      721 cgtgcccgtg gaggtcaccc tccgcggctg ccacccacac acgaaataac cccccttcaa
      781 cccgctgatc cacgaagcaa ggtgacgtcc atggcaggcc acggtcgcaa gcaccctccc
      841 atccccgtga tcgtcgttct cgtcgtcgcg ctcgcgggcg gaggctggtg gtggtggcgc
      901 acggccaacc agcccgaggc atcggccagc accgcgctca acgggcaggt cgaagccagg
      961 cagtacgaca tctcctcggc catcgccggc cgggtgctca ccgtgcctgt ggcggagggc
     1021 gaccaggtga ccaagggcca gcccatcgtc accctcgaca ccgccgcgct cgacctgcaa
     1081 ctcaagcagg cccagcaggg ggtgatcgcc gcccaggccg cggtcaccaa cgccaaggac
     1141 tccggcacca aggccgacgt caccgccgcc gaggcgcggg tcgagcaggc caacgccgcc
     1201 gtctcgctcg cgcaggtgca actcggctac gcctccgtga gcgcacctcg cgacggccgg
     1261 gtcgtcacgg tcgtcaccaa cgtcggccag aacgccgccc ccgggcggat cctcgcctcg
     1321 atcgtcgatc tgcacgacct gttcgtccgg gtctatgttc ccgagacacg catcggcacg
     1381 gtgtcggtcg gtcaggcggt gtcggtgtcc tccgactccg gcggtgccgc catcgcaggc
     1441 accgtctcct tcgtctcggc gcaggcggag ttcaccccca acaccgtgca gacggctgag
     1501 cagcgggcga agctcgttta cgaggtgcgg gtcaccatct ctgactcctc ggggaccctc
     1561 aaaccgggta tgccggtcga cgtcaccttg cgctgacatg gccatcactg gagccagccc
     1621 tgccgcaccg gcggtgtcga cgcagggact cacccgacgc ttcggcgacg tcgtggcggt
     1681 cgacggggtc gacctcgtcg tgcagcgcgg cgcggtcttt ggtctgctcg gtccggacgg
     1741 ggccggcaag tcgacgctca tccggatgct ggccaccgtg ctgctgccgg acgcggggga
     1801 ggccttggtc ctggggcatt cgaccgcctc tgaggcctcg gccatcaccg gccggatcgg
     1861 ctatatgtcg caacggtttt cgatgtatcc cgacctcacc gtcgccgaga accttgactt
     1921 cttcgcgacc gtccgaggcg tcccgaagca ggtccggcgc gagcgatccg cggccttgct
     1981 ggacagcatg gggctggccg agttcggcgg tcgccaggcg cggcatctgt cggggggaat
     2041 gaagcagaag ctcatgctcg ccaccaccct catgcacgag cccgagttgc tcctgctcga
     2101 cgaacccacc actggtgtcg acccggtgtc gcgccgggag ttctggcggg tgctggccgg
     2161 gttgcaccgg ggcggcacca ccgtcgtcgt ggccacgccc tatatggacg aggctgagcg
     2221 ttgctcggcg atcgccttcc tccaggacgg acggatccag caacagggca cgccggagga
     2281 gatcaccgcc ctcgttcccg gcaccctggt cgaggtgtcg ggagccgacc cgcgagccgc
     2341 tctcgccgcg ctcgccgacc tgccgggagt cggctcaccc cacgtcttcg gcgaggtcgt
     2401 ccgagtgctc tgggagggct cgcttgcgag cgtcgagggg gccatcacga cccacttgcg
     2461 ggcggccgga ttccccacgg tccgggtgac tcccacgcgc gtcgacatgg agaccgcctt
     2521 cgcggtcctc gccgaggccg gccgatgagc ggcgcaggag tccggatcgg cgccgtggtg
     2581 cgcaaggagt tcatccacgt cgggcgcgac ccgcggctgc tcgcggtcgt cctcatcatg
     2641 ccgatcatcc aactgctgct cttcgcctac gcgatcagct tcgatgtccg aaacctgccc
     2701 accgtcgtcg tcgacctgga ccacacgccc gacagcgcgg catacctgcg ggcgtatgag
     2761 gcgtccgggc tgttcgacgt ggtcgggcac ggcgccgacc cgactgcggt cgacacggcg
     2821 ttcgaccgca acgaggccca ggtggctgtc atcgtccccg ccgggttcgc tgcgtctctc
     2881 gcgcgcggtg agcaggccca ggtgggagtc ctcgtcgacg gcagcgagcc caactcggcg
     2941 cgcgtcgcgc agacctacag catggctctc aaccagctct atgggcgcgc gctcgctgtc
     3001 gaatgggcgt cgacccaagg ggtcgacctc ggccaaatcg gtttggtgga accgagattg
     3061 cgcacgtggt acaaccctga tcgggcatcg tcgatcttcc tcgtgcccgg gctcatggtg
     3121 gtcatcatca tgatcgtcac cgcccagcag accgctgtga cgctggtgcg cgagcgggag
     3181 cagggcaccg ccgagcaact catggtcagc cccctgcggc ggatcgagct catgatcggc
     3241 aagctgactc cgtgggtcat catggcgttc ctcgacatcg tcgttatcgc cgtgctcggg
     3301 gtggtcctct tcggggtgcc gatgcggggc gaccccgtgg cgttggcggt cggtgcgacc
     3361 ctctttgtgg cctcctgcct gggcatcggc ctcatcgtct cggccgtcgc gccgagcctc
     3421 gacacggcca acatggcggc cttcctcgtc gcgttcctgc cggcgttcct gttgtccggg
     3481 ttcgcctttg cgctcgatca gatcccgccg gtccttgagt ggatcagtcg gctcttcccg
     3541 gcccgctaca tggtgacgat ctcgcgctcg gtgctgctca agggcgccgg cttccccgag
     3601 gtctggccgc aggtggcggc gctcgcggca tacgccctca tcagcctgct catcgcggtc
     3661 cggctgcacg ggaggcgcgc atgagcctga cccgcatccg cctgctggtg tggaaggagt
     3721 tcctccagct gcggcgtgac ccgctgttgt tgcggctgct gctgctcatg ccgatcatcc
     3781 aactcgtcgg cttcgggtac gtcgtgtccg ttgacgtgcg caacctgccg acggccgtcg
     3841 tcgacctcga ccagacggtc gtctcgcgcc agctggagtc gagtttcacg gcgtcgggct
     3901 acttcttggt cgtcgcgcgg ccggacgccg agactcagct gacttcgttg ctggacaccg
     3961 gagttgcgaa ggtggcgctc gtcatcccgc agggcacgca ggacctcctt gcgcgcggcc
     4021 agactgcgcc ggtcgggatc gtcgtcgatg ggtccgactc gcagatcgcc agcgtgggca
     4081 gtgggtatgc cgcgcaagcc atcgcccgct tcaacgcctc gcggacggcg gcgttcacgg
     4141 ggtcgctgca agcgccgggg atcgatgcgc ggacgcgggt ggtgttcaac ccgacgctcg
     4201 acccgatcaa cacgatgatc cctgggctgg tcgccgcgat cctcatgatc tcgctcatgt
     4261 cgatcatgtc ccaggcggtc gtgcgggaac gcgagtcggg gacgttggag cagatgttcg
     4321 tcacgccaat tcgcccgtcg gagtatctca ttggcaaggt ggcgccctat gcgctgctcg
     4381 ccgtcgcgca gctgagcgtt gtcgccgcca tcgggatgct gtggttcaag gtgccgttca
     4441 acgggtcggt gtgggtggtg ttcgtcgggc tcgcgctgtt cctgctcatc tcggtggggc
     4501 tgggattgct cgtctcgctc atctcccgaa cccgccaaca ggcccagcag gcattggttt
     4561 tcatcatgat cccgacgatg gtgctgtccg gtttcatctt ccccatcgag tcgatgccgg
     4621 aggcgatcca acccctcacg tatgccgtgc cgctgcggta tgcgctcacg gtgttgcggg
     4681 ggtcgttcgt caagggcagc ggcttcgagg cgcttgctac gccgctgtgg gccatggtcg
     4741 gtttcgcgat cgtcatcttc ggggcggccg tcatcgccac ccgtcgccgg atcgctgagt
     4801 gagggggtgt tgtgatgacc cgggaaggtg aggttcacgg catacccgct gatccgtctg
     4861 cggcgatctc ggtgagggga ctgaccaaga cgttcggcga cttcacggcg gtcgacggga
     4921 tcgacctgga cgtgccgcgc gggcaggtgt tcggcttcct cgggccgaac gggtcgggga
     4981 agacgacgac gatccgcatc ctcacgggga cgtccgcgcc tcgcagcggg caggcgttgg
     5041 tgctcggcga ggacgtcgtc gcgcatccgg agcgggtgcg gccgcggatc ggatacatgt
     5101 cgcagaagtt cgcgctgttc gaggacctca cggtcgatga gaacctgcgc ttctatgccg
     5161 gcgtctatgc gctggacccg gcggtgtttg ctgcgcgtcg gcgctacatc ctcgagatgg
     5221 ccgacctgca ggggcgcgag cgggagctca cgcgcaacct ctcggtcggg tggcggcaac
     5281 ggctcgcgct cggggcggcg accatccacg agccggattt gttgtttctg gatgaaccga
     5341 cctccggcgt ggatcccgtt gcgcggcggc agttctggga cctgctctat gagctggccg
     5401 ggcgtggggt gacgctgttc gtgacgacgc actacatgga ggaggcgagc cactgccacc
     5461 ggctggcctt catctatcgg ggtcggatcg tcgcggaggg tagcccgaac gagatccgcg
     5521 ccaacctctc gacgcaacgg gtctatgacg tcacggtccg ggatgccgac ctggcgctgg
     5581 gttggttgga gacggccgac gggatcgcgg aggcctatct gtcgggggcg gctctgcacg
     5641 cggtgatcga ccgtggagtg gatcgggggg cgggcgcgtt gcgcgagcgg atggtggccg
     5701 cgggcttcgc cgatgccgtc gtcgtgtctg tcgagccgac catcgaggac gtgttcgtca
     5761 acctcgtcag ccgcgagcgc gaggcatagc cacttgggat tgtggcgggt cgggtcgggc
     5821 cctcgactgt gcgggggacg gcccagcaag gttcgtcaat gacggaggcg gacaactgct
     5881 cgcagaagca gccgggcgct gaccaggccc accaggctgg ccatgccaat ccgctgttgg
     5941 agggcggtgg cgcgggcgat gtccccggtc gaaaccggcg gcccgtcgcc cagccgcccg
     6001 cgttcctcgc ggacgcccgc gtagtcattg gtcccgccga gttgccggcc cagggcagca
     6061 gcgaacgccg cctccacggg accggcgttg gggctcgggt gggcaggggc gtcgcggcgg
     6121 accacggcat acacctgccg tgcgcccgtc agcgaccatg tggtgagcgc cgtcatggcg
     6181 accgtgaccc gcgccggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgg
     6301 ccgacgaggt tgcggatccg cagcctcgcg cccgccaggt cgtctgcctg cagcagggca
     6361 tccatggcct cggcttcgcg ggccagtgag cgccctccga ggactgccca cgtcgccgcg
     6421 gcaaccaccg ggacccgagc agcccgcggg agccgggtca gcgcctgccc gagggccact
     6481 gcgcccccga ccaacaccgc ctcgtgaacg atcccagacg agcgccgcgg cgcataggta
     6541 tgccgttcga gcgcctccgc cactcgtccg aacccggcga ccgggtgcca gcgccggggg
     6601 tcaccaaagg cgaggtccgc ggcatacccg aggacgaggc cgacggcgac gtcgtccagg
     6661 ctcatggcgc ctttccccat cgagccgtgt ccccggccgc cgatccccgt catcgccgca
     6721 gacccccgcg aagccggacc tcggcgaggg cgcggacgag ggcgtcgctg gtcgccgcgt
     6781 cgcggaccgc gatccgcacc cagccgccgt cgagcccggg gaaggtgtcg ccgcggcgca
     6841 cggcataccc ctgttcgcga agttccgcgt ggatgcccgg gccgacctcg acaaggacga
     6901 acggtgcctc gcccgggacg ggccgcagac cgatcgactc gagagcctgg actaggtggc
     6961 cccggtcacg ggctatcgcc tcggcgaccc gcgcgacctc ggcgagggcc tcgggggtcg
     7021 ctgtcgcggc catgacggcg gcggccgggg tggacaccga ccagggggac tgctgctcgg
     7081 ccagggccgc gaccaggtct ggatcgccga cgacgtaacc cgcgcgcagc ccggcgaccc
     7141 cccacgtctt ggtgagcgag cggaggacga gaacgcccgc caggtcggga ctgatgaggc
     7201 tctcccgctc gccgcggatc gcgtcggcga aggcctcgtc gacaaccagg acgcgaccac
     7261 gcgcgcgcag agcctccagg gtggcgcgcg ggtgcaggac gccggtcggg ttcgtggggt
     7321 tgccgacgag gacgaggtcg gcccgtgggt ccacggcggc cggatcgaga gtaaagcccg
     7381 acttggcctg cagcacatgc cgatcgggca cgcggccggc ccggcgcagg gcggcctcgg
     7441 gctcggtgaa ctgcggatgg acgaccagtg ggcgccgggc tgcgatggcg cgcgcgatca
     7501 gcgtgaacgc ctccgcgccg ccactggtcg gcaggaccat gtcctccggg accgcgtgtc
     7561 ggcgggcgag ggcggcgcga gcgaccgagg gatctgggta tgccgcgagc tgcccggtcg
     7621 tgtcccgaat gaccgtcgcg agccaggccg gcggggttcc gacccggacg ttgacggcga
     7681 ggtcgaccag gtgggtggcc cccctgacct ccgcgtcccc gtggtggtcc aggcgctcgg
     7741 tcacctcgcg accctatcgg tgtctccgag gcgtcgtcct acactcacga cgtgccctgc
     7801 gtgtgtggcc gaggcgcccg atgggatcca ccggccctca ggtggtggac cgcgagctga
     7861 cctgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcgacc ccgactgcgc
     7981 actcgcgcac tgggggatcg ccttcgcgct cggcccgaac tacaacaagc cgtgggagtt
     8041 cttcgcgccc gacgaggcga cggcggtcct ggaacgggcg cgcgatgagg cgcgggttgc
     8101 ggcggactgc gcatcggggg gcacggcggt cgagcaggcg ctgaccggag ccttgctggc
     8161 ccggtatccc gacggccgtc cgatcgagga gtgcacggcc tgggaggtgg agtatgccgc
     8221 cgccatgggc caggtgtatg ccgcgcatgg cggcgacctc gacgtggcct gcttctacgc
     8281 cgaggcgctg atgaacctca cggcgtggca gttgtgggac cagcgcaccg gggagccggc
     8341 cgaagggtcg cgaaccctgg aggcgaaggc ggttcttgag gccgcgctgg ctgcccccgg
     8401 gggctcgcag catccgggcc tcctgcacat gtacatccac ctgatggaga tgtcgccgac
     8461 ccccgagagg gcgctgccgg tcgcggacct gctccgagga ctggtgccgg acgcggggca
     8521 tctgcaacac atgccgaccc accttgatgt gctgtgcggc gactaccgcg gtgtggtggc
     8581 atcgaacagc gccgcgatcg cggccgacgc ggcgtttgtc gcccagcggg ggccgctcaa
     8641 cttctacacg ctctatcggg cgcacaacct gcacttcaag atctacggcg cgctgttcct
     8701 cgggcaggag cggacggctc tcgccacggt tgatcagctc gaagcggcga tcccggagga
     8761 gttgctgcgc gtgcagttgc cgccgatggc cgactggctg gagggcttcg tgccgatgcg
     8821 ggtgcatgcc ctggtccggt tcggtcggtg ggatgcgttg atcggcttgc cgttgcccgc
     8881 ggaccaggat ctctactgca ccaccacggc gatgctgcac tacggcaagg gtgtggcctt
     8941 ctcggcgacc gggcgggtcg ccgaggcgga ggaggagcgt cggctgttct tggctgccgt
     9001 ggagcgggtc ccggagtcgc ggatgctgtt caacaacacc tgccgcgaca tcctggccgt
     9061 ggcaagcgag atgctcgacg gcgagctcga gtatcgaaaa ggcaaccacg acagtgcgtt
     9121 tgcgcacttg cgccgttcgg tcgagttgga cgatggtctg ccctacgacg agccgtgggg
     9181 ttggatgcag ccgacccggc acgcgctcgg tgcgctgctg ttggagcaag ggcacgtcgg
     9241 ggaggcggag gccgtctatc gcgccgacct tgggctcgac gcgaccttgc cgagggcttg
     9301 ccagcacccc ggcaacgtct gggcgctgca cggctatcac gagtgcctgg ttcggcttgc
     9361 caaacacgac ctcgccgagg tcgtcgcgca gcaactgaag ctggccggcg cacgcgccga
     9421 cgagccgatc acggcgtcgt gcctatgccg gatcgatcga tcaggggcgc tgctggcggt
     9481 cgacacctgt tgctccggcg aacctccttc cggccccgcc gaacccgcga ccggctgttg
     9541 ctcccactga cccccccagc ggttgggcgc caagtcgttc actcataggg tggccgccat
     9601 ggagatgacg gagccggtgc gggcggcagt gcagtcggcc gtgctgtgtt ggctcgccac
     9661 ggcggacgcc gacggtcgcc cgagcgtgtc ccccaaggag atcttcgcgg tggtcggcga
     9721 cgacatcgtc atcgctcaca tcgcgtctcc gcggtccgtc cgcaacatcg caggcaaccc
     9781 gcaggtgtgc ctcagcgtgc tcgatgtgtt cgagcagcgc ggctaccgga tcgcgggccg
     9841 ggcctcggtc gtcaccccgg atcacgacgc tttccctacc gtggtggcgc ccttgcggga
     9901 gttggccgga gccgccttcc cgatcaaggc cgtgattcga gttgccgtgc aagacgtgga
     9961 acggttgtcg gctcccagcc tctggatgta ccccgacgtc gacccggcac agcgacgagc
    10021 cggtgtcctg gaggcctacg gagttgtcga cgcgccctca ccgggatgag ggagggatag
    10081 ccggcttgtg ggtgccggcc atccactcgc gacgaccgag aggcactcgt cagcagcgac
    10141 ctccgaagca ctccaagacg ccggtgtagt ggccgacgat ggtgccctgc cctgctctgt
    10201 tcgaggcccc gagtcaccaa agaaacgatg gtggcactcg cggtttcgtc cagctacggg
    10261 ctggccgaaa cacgcgggtg cccggcgggt gctactgcca ggggtgtagg tgggggatga
    10321 ggctggtcat cgcgccgacg atctggcggg ccttgggcag ttcacctcgg cgcgcgccac
    10381 cgcagcagcg gcaagagcaa cggcgaacct tcctttcatg aaaccgatcc tgggatcgga
    10441 aacacgaaac tggaccctca gcggccaccc gcgtcgtgtc cgatccggcg cgggggttgg
    10501 aggcggcaga ggacggcgcc gatgaggagc acgacggccg agactccgag gccccattgt
    10561 tggccgccgg tacggtcggc gagcagtccg gtgaaccagg gcccgatgac ctggcccagg
    10621 gcgaacaccg tggtgagcat cccgagcgcg gcggtcgtgt cagccgggtc gacggcgtcg
    10681 cgacctgcgg cggtgacggc cccgacgagc gagaggaagc acaacccgaa cagcacgccc
    10741 gacgcataga gcgcgggggt ggcggacgtg agggcgggta tgccggtcgc gacggcgagc
    10801 agcacgaaga ggactgccgc cgcggagcct ccgcgggcac cccgcagcag ccgcgcccag
    10861 acctgcccgc tcaccgcccc ggccacgccg agcgcaaccc agaacgcggc aatctccttg
    10921 ggagacagcc cgacctgggt caacatggcg atgatgaacg tcatgtagcc gatgtagccg
    10981 gcaccgaaca acaggtagcc gctagcaagt cggcccaggc cgctgaccca gaagctccgc
    11041 gacccggggg ccgctgcggg agtggtgggg gcggcatacg cggcgatggt ggcggccacc
    11101 gtgccgaccc ccgcgaggat cgcaagcacc agccacccgg tgggccatcc cgccggcccg
    11161 agccgctcga acaccgcggg gacgagcagg ccggaggcga cgatccccac cccgccgccc
    11221 gcgaagtagg tcccgatgag cacggccgcg cggtgcgggg tatgccgcct gctgatcgtc
    11281 gaggtgatcg cgccaccgag gacgaaggcg cctgcaccag cgatgccgag gatcgtgcgg
    11341 accgccagga ggacttccag ggaacttgac gcggcggtcg cggcgagg
//
LOCUS       DAOMZY010000071        11384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5339_length_11384_cov_5.647807, whole genome shotgun sequence.
ACCESSION   DAOMZY010000071 DAOMZY010000000
VERSION     DAOMZY010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 11384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11384
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5339_length_11384_cov_5.647807"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /gene="ftsY"
                     /locus_tag="PLK64_05715"
     CDS             <1..931
                     /gene="ftsY"
                     /locus_tag="PLK64_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPV79271.1"
                     /translation="KPESARGRLARLRARLARSNSAIGKGLLALLSRGGLDEAAWEDV
                     EDTLLTADLGVEASTELVESLKTKVKVEGTTDESVVRGWLREDLLRLVDPSMDRRVAS
                     TRVGDRPAVILVVGVNGTGKTTTVGKLARVLVAEDRDVVLGAADTFRAAAADQLATWG
                     DRVGVPTIRSDREGADPAAVAFDAVKAAAEMEADVVLIDTAGRLHNKTNLMDELGKVK
                     RVIEKRSPIDEVLLVLDATTGQNGLEQARVFADVVDVTGIVLTKLDGTAKGGIVVAVQ
                     RKLGVPVKLVGLGEGPDDLAPFDPAAFVDALLD"
     gene            1097..2524
                     /locus_tag="PLK64_05720"
     CDS             1097..2524
                     /locus_tag="PLK64_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HPV79272.1"
                     /translation="MSTPLMPLADATATIDYAATTFVILCASLVLLMTVPALALFYGG
                     MTRSKSVLNMMMMSFGAAGLVGVLYVLYGYSMSFGTENIAGIIANPFEKFGLAGTDGL
                     VNPFGYEGYGNIPELALVAFQLTFAVITVALISGAIADRVKFSTWMVFAGLWVTLAYF
                     PMAHMVWGGGLLSAADGGLSAAIFGSTDGKADVVPIDFAGGTVVHINAGMAGLVLALL
                     VGLRLGFGKVAMRPHNVPLTMIGAGLLWFGWFGFNAGSELAADGTAALAWVNTTAATA
                     AAMLGWLLVEKVRDGHATSVGAASGIVAGLVAITPACGALNPLGSLILGAIAGGLCAL
                     AVGLKYKFRYDDSLDVVGVHLVAGLWGTVGAGLLSTTTGLFYGGGIRQTLLQIVIALV
                     TVVFSGVVTLVLGLILKATMGWRIDKDAETSGIDQDVHAESAYDLTASSGGRFGGAFA
                     DAGITKSSTSSTPAPPASITEGASA"
     gene            2521..2859
                     /locus_tag="PLK64_05725"
     CDS             2521..2859
                     /locus_tag="PLK64_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HPV79273.1"
                     /translation="MKLVTAIIKPHQLDTVKEALEAYGISGMTISEASGFGRQKGHSE
                     VYRGAEYTVDFVPKVRLEVLSDDVDTAGVIDVILKAAQTGRIGDGKIWSVPVHDLVRV
                     RTGERGVDAI"
     gene            2915..5323
                     /locus_tag="PLK64_05730"
     CDS             2915..5323
                     /locus_tag="PLK64_05730"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HPV79274.1"
                     /translation="MTDVSQRRLDVAGTRSFAAAGAGPARRAALAEFARTWLVDRWAE
                     AAQGRPTPGLALAAVGSIARGDAGPLSDYDLVLLHEGRAMSQKDIDRFADRLWYPMWD
                     SGIKIDHSVRTLGQCRQVAGADLSAAVGLLDLSHLAGEENLVAAARSAVAQDWRANAR
                     RRLPEVLDAVTARHTRMGDCAHLIEPDLKEARGGLRDMTVLRAMTAAWLADRPHGEVD
                     AAYLRILDVRDAVHMVTGRARDRLGREDHDAVAALLGYDDTDLMLTDLSQAARIVGYA
                     LDGTLRRASQSQRARVLRVGPRRPSLEALGYGLFLHDGEAVLGPGVDPHANPTLALRA
                     AGIAARAGVSLAPATLANLAAGCPPLPQPWPADALALFTDLLAAGPGLVGVWEGLDLA
                     GIIEQWIPEWREVRSRPQHNAVHRHTVDRHSIEAVVVAGGLVRDVQRPDLLLLAALLH
                     DIGKVAGAADHAEVGAPVADRILGRLGVADADRALVVRLVREHLTLVELATRRDPHDP
                     ATVASLSAAVDGSLETLALLRALTEADACAAGPAAWTDWRAQLVARLTAVGARALRER
                     PERPERPGAEAVGGIGEGGLGDDGPGATAAEIAEVAAGRPVVRVDRLGTAHRLHIVDN
                     DRTGLFADTTGLLAATGFIVRSALLRTVDGIAVNEWHVDSPSGDAPDGGMLSRGLGRL
                     AMGDRSPLAPLARRRRALEVTAHASLGRPGMTRAMVLPEASRQSTVIEVRATDRRGLL
                     HDLGSAITDAGLSVRSAHIATYAGQALDTFYLTRADGSPLAPAEVGRTIALLIDTSDG
                     RAAS"
     gene            5410..7032
                     /gene="ffh"
                     /locus_tag="PLK64_05735"
     CDS             5410..7032
                     /gene="ffh"
                     /locus_tag="PLK64_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPV79275.1"
                     /translation="MFNNLSDRLTATFRNLRGKGRLTEHDINATLRDIRMALLDADVA
                     LPVVKAFVAAIRERALGAEVSQALNPGQQVVKIVNEELVTILGGQTRPLNLAKNPPTV
                     IMLAGLQGSGKTTLAGKLGYWLKEQGHTPMLVAADLQRPNAVTQLEVVGQRAGVPVYA
                     PERGNVAGHDAVGGAGEGTRSYGDPVKVADDGIQYAIARQHDVVIVDTAGRLAVDADL
                     MAQAKDIRAAISPDEVLFVIDAMIGQAAVETAQAFQEGVGFTGVVLSKLDGDARGGAA
                     LSIAHVTGRPILFSSIGEGVKDFEVFHPDRMASRILDLGDVLTLIEQAERAFDKQHAD
                     EMARKFLAEEDFTFDDFLEQMAAIKRMGNFKSMLGMLPGMQQMRAQLDAFDEKEFDRV
                     EAMVRSMTPFERSHPKTINGSRRQRIARGSGMQVSDVNQLLERFTQAQKMMKSLARGG
                     GPGLPGMPGMPGMRKGKAAPPPRKKAKSGNPAKRAAEERALADRATTGRQEAAGRAFG
                     MPPGAGGASGAPGAADLDPSKLPPGFEKFLGR"
     gene            7360..8322
                     /locus_tag="PLK64_05740"
     CDS             7360..8322
                     /locus_tag="PLK64_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79276.1"
                     /translation="MTLEQEVATGQWGIVSRAQLSAAGMSPEAIRWRAARQWRSLLPG
                     VYAIEPGRLGVHQAAMAAQLYVGPSGLLGGSSAARLHGVESVRLTGLIDVVVPMNGRT
                     RTIGHVRIPRTRFSEPVPHHHGSLRLMSPARAVAEALRRMPTDEVAAEVAIEAVQRRI
                     AALDDLRHWTSLLGRAGSARAERALSVVASGAWSVPEHRLAHAVTGVKGLPGLWLNPT
                     LKDEHGAQLISPDLWFDEVALAVMVHSRRYHSTGDQWDATVTSDAELSAAGVVVVGVT
                     PHQISRAMPEVLARIARAYAAASRRPRPGVKAVPRGRDGVRLVG"
     gene            8390..9229
                     /locus_tag="PLK64_05745"
     CDS             8390..9229
                     /locus_tag="PLK64_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPV79277.1"
                     /translation="MTAATSRRLHTLAAGTSGPRIAFLHGLFGQGRNWSQIAKALSGP
                     DGDQAQCLLVDLPDHGHSPWSDHFSLQAYAASVAATLRATAPGERWTLVGHSLGGKVA
                     MIVALSQPDLIERLAVIDIAPRHYRSHQWFVDYVNVMRALPLDSLPDRASANAALAQH
                     IPDPAVCAFLLQNLRRHGTAWSWQINFDVLARPGTGPAGVAGWPTADATAYQPFRGPV
                     AWVAGAESTYITDRDAEPMRALFPRVTQVTVKDAGHWVHSDQPEVVTQILRRFALSTA
                     RRH"
     gene            9250..10338
                     /locus_tag="PLK64_05750"
     CDS             9250..10338
                     /locus_tag="PLK64_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPV79278.1"
                     /translation="MATVLHIVGEILVGPADTRPECWVLDGRVTYAAPTGPGHDVQTV
                     RGWALPGLVDAHCHVGLDHHGPVEPDVAEQQATADRDAGTLLIRDSGSPADTRWIDDR
                     DDLPRIVRAGKHIARTRRYIRNYAHEIEPDELVAHVRMEARRGDGWVKLVGDWIDRET
                     GDLAPCWPREAVIAAVAAAHEEGARTTAHCFAEESLHDFAAAGTDCIEHASGLDEEST
                     KAFAAQGIAIVPTLINIANFPELAAPAAEKFPRYHAHMLDLHARRFETIARAHEAGIP
                     IYVGTDAGGVLPHGLAAAEALELAKAGLSTLDVLGAGSWKAREWLGHEGIEEGASADF
                     VVYPADPRADLAVLGAPDAIILRGNRVR"
     gene            10368..>11384
                     /locus_tag="PLK64_05755"
     CDS             10368..>11384
                     /locus_tag="PLK64_05755"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV79279.1"
                     /translation="MRVAPLTADGLPGLRRAMALSQGRVGVWGPTGLDSVDDLLAVQG
                     RSKRTFIVHALAPDSSAGHGIAALINVNDIVMGRFASATLGYDAYDPYAGRGLTRDGL
                     ALVLDVCFGPPPDGLGLHRVEASVQPANVRSAGLLRSLGFLREGFSRRLLRLPSLGDE
                     EVDWRDHDRYALLCEDWPGAAESARVAYRAAPLRPVVCVVNGLPGSGKSTLAPRLAVE
                     LGVPLLGKDLIKEAVADALDESDQGRLSKELGSGASRALWALLAASPAGGVVETWLPP
                     TRDTAYLVDGLRAAGLDPTRVPEVFCDVPPEVARERDLVRAARGERHPVHRGPVEETE
                     WHESV"
BASE COUNT         1668 a         4012 c         3944 g         1760 t
ORIGIN      
        1 gaagccggag agtgcgcgcg ggcggttggc gcggctgcgt gcccgcctcg cccggagcaa
       61 ctccgccatc ggcaaggggc tgttggctct gctgtcccgg ggaggtcttg acgaggccgc
      121 ctgggaggac gttgaggaca ccctgctgac cgccgacctc ggcgtcgagg cgagcaccga
      181 actggtcgaa tcgctcaaga ccaaggtcaa ggtcgagggc accaccgacg agtccgtggt
      241 gcgtggctgg ttgcgtgaag acctgctgcg cttggtcgac ccgagcatgg accgtcgggt
      301 cgcctccacc cgggtggggg atcgccccgc cgtgatcctc gtcgtgggcg tcaacggcac
      361 cggcaagacg acaacggttg gcaagctcgc ccgggtgctc gtcgccgagg accgcgacgt
      421 cgtgctcggc gcggccgaca ccttccgcgc cgccgccgcc gaccaattgg ccacttgggg
      481 tgaccgggtg ggcgtgccga cgatccgctc cgaccgcgag ggcgccgacc ccgcggccgt
      541 ggccttcgac gcggtcaagg cggcggccga gatggaggcc gatgtcgtcc tcatcgacac
      601 cgctggccga ctgcacaaca agaccaacct catggacgag ctcggcaagg tcaagcgcgt
      661 catcgagaag cgcagcccga tcgacgaggt gctgctcgtg ctggacgcca cgaccggcca
      721 gaacggcctc gagcaagccc gcgtgtttgc cgacgtcgtc gacgtcaccg gcatcgtcct
      781 caccaagctc gatggcaccg ccaagggcgg catcgtcgtc gcggtccagc gcaaactcgg
      841 ggtcccggtc aagctcgtcg ggctcggcga aggaccggac gacttggcgc ccttcgaccc
      901 cgctgcgttc gtcgacgcgc tgttggactg agcgcggcgc tcgtcgcgcc gcacggtggg
      961 tggggcgcga cgcggcatac cgctgcggta tgccgcgtcg cccccgaaac gtgatcctta
     1021 cctggcgtcg gcgtcgataa cacgcccgaa acaatcctgg gcgtccccgg aaacccgggc
     1081 gctccatcgt taccgcatga gcacaccact gatgccattg gcagacgcca cggcgactat
     1141 cgactatgcg gcgacgacct tcgtcatcct gtgcgcgtcg ctcgtccttc tcatgacggt
     1201 tcctgcgctg gccctcttct acggaggcat gacccgctcg aaatccgtcc tcaacatgat
     1261 gatgatgtcc ttcggagctg cggggctcgt cggcgtcctc tacgtcctct acggctactc
     1321 gatgtccttc gggaccgaga acatcgccgg gatcatcgcc aacccgttcg agaagttcgg
     1381 gctggccggg accgacgggt tggtcaaccc gttcggctac gagggctacg ggaacatccc
     1441 cgaactcgcc ctcgtcgcct tccagttgac cttcgccgtc atcacggtgg ccctcatcag
     1501 cggtgcgatc gccgaccggg tgaagttctc gacctggatg gtgttcgcgg gcctgtgggt
     1561 cacgctggcc tacttcccga tggcgcacat ggtctggggc ggcggcctgc tgtcggccgc
     1621 cgacggtggc ctgtcggccg cgatcttcgg gtcgaccgac ggcaaggcgg acgtcgtccc
     1681 gatcgacttc gctggtggga cggtcgtcca catcaacgcc ggtatggccg gcctggtcct
     1741 ggccctcctg gtcggactgc gcctcggctt cggcaaggtc gcgatgcggc cgcacaacgt
     1801 cccgctgacg atgattggcg ccgggctgct gtggttcggc tggttcgggt tcaacgccgg
     1861 ctcggagttg gctgccgacg gaaccgccgc cctcgcgtgg gtcaacacga ccgccgcgac
     1921 cgcggctgcg atgctcggct ggctcctggt ggagaaggtg cgggacgggc acgcgacgtc
     1981 ggtcggcgcg gcctccggga tcgtcgccgg actcgtcgcg atcacgcccg cctgtggggc
     2041 cctcaacccg ctcggctcgc tcatcctcgg tgctatcgcc ggtgggctgt gcgcgctggc
     2101 cgtgggcctg aagtacaagt tccgctacga cgacagcctt gacgtcgtcg gtgtccacct
     2161 cgtggccggc ctctggggga cggtgggcgc gggcctgctc tccaccacca ccggtctgtt
     2221 ctacggtggc ggtattcgcc agaccctcct gcagatcgtt atcgctctcg tgaccgtcgt
     2281 cttctccgga gtcgtcaccc tcgtcctcgg tctcatcctc aaggccacga tgggatggcg
     2341 gatcgacaag gacgccgaga cctcgggcat cgaccaggac gtgcacgccg agtccgccta
     2401 cgacctcacc gccagcagcg gtggccggtt cggcggggcc ttcgccgacg ccggaattac
     2461 caagtcgtcc acgtcgtcca cgcccgcccc gcccgcctcg atcacggaag gagcgagcgc
     2521 atgaagctcg tcaccgccat catcaagccc caccagctcg acaccgtcaa ggaggccctc
     2581 gaggcctacg gcatctcggg catgacgatc agcgaggcct cgggcttcgg ccgccagaag
     2641 ggccacagcg aggtctaccg aggcgccgag tacaccgtcg acttcgtgcc gaaggtgcgt
     2701 ctggaggttc tgtccgacga cgtcgacacc gcaggtgtca tcgacgtcat cctcaaggcc
     2761 gcccagaccg gacggatcgg ggatggcaag atctggtcgg ttcccgtgca tgatctggtg
     2821 cgtgtccgca ccggcgagcg cggcgtcgac gccatttgac gtcacctgca cgccggtggt
     2881 gtccgagcac tgacgacaag gaagttcgga tcccatgacc gacgtttcgc aacgacggct
     2941 cgacgtggcc ggcacccgca gcttcgctgc tgcgggtgcc ggtccggcgc gtcgggccgc
     3001 gctcgccgaa ttcgcccgca cctggctggt ggatcgctgg gccgaagccg cgcagggccg
     3061 cccgacgccg ggcctggccc tggctgcggt gggaagcatc gcccggggcg atgcgggacc
     3121 actgagtgat tacgacctcg tcctgctcca tgagggccgg gcaatgagcc agaaggacat
     3181 cgaccgcttc gccgatcggc tctggtatcc catgtgggac tccgggatca agatcgacca
     3241 ttcggtccgg accctgggcc agtgccgcca ggtggccggc gccgacctgt ccgcggcggt
     3301 cggactgctc gacctcagcc accttgcggg ggaggagaac ctcgtcgccg cggcccgctc
     3361 ggcggtcgcc caggactggc gcgccaatgc ccggcggcgc ctgccggagg tgctcgacgc
     3421 ggtgactgca cggcataccc ggatggggga ctgcgcgcac ctcatcgagc cggacctcaa
     3481 ggaggctcgc ggcggactgc gcgacatgac cgtcctgagg gccatgacgg cagcatggct
     3541 cgccgaccgt ccgcacggtg aggtcgacgc ggcatacctg cgcatcctcg acgtgcgcga
     3601 tgccgtccac atggtcaccg gccgggcccg cgaccgactc ggtcgcgagg accacgacgc
     3661 cgtcgcggcc ctgctcggtt atgacgacac cgacctcatg ctcactgatc tgtcgcaggc
     3721 tgcgcgcatc gtgggctacg cgctcgacgg aacgctccgg cgcgcctcgc aatcccagcg
     3781 ggcccgggtg ctgcgggtcg ggccgcgtcg gccgagcctg gaagccctcg gttacggcct
     3841 gttcctgcac gacggcgaag cggtgctcgg gccgggcgtc gacccccacg ccaaccccac
     3901 gttggcgctg cgggcggccg ggatcgctgc acgtgcaggg gtgtcactgg ccccggccac
     3961 cctggcgaac ctcgcggcgg gctgcccgcc gctaccgcag ccctggccgg ctgacgcgct
     4021 ggctctcttc accgacctgc tcgctgccgg tccgggcctg gtcggggtct gggagggctt
     4081 ggacctggcc ggcatcatcg agcagtggat cccggaatgg cgcgaggtcc gcagccggcc
     4141 gcagcacaac gcggtccacc ggcacaccgt cgaccgacac tcgatcgagg cggtcgtcgt
     4201 ggccggcggg ttggtgcgcg atgtccagcg ccccgacctc ttgctgctgg ccgcgctgct
     4261 gcacgacatc ggcaaggtcg ccggcgccgc cgatcacgcg gaggtcggtg ccccggtcgc
     4321 cgatcggatc ctcggtcgac tgggggttgc ggacgccgat cgggcgctcg ttgtgcggct
     4381 cgtgcgcgaa cacctgaccc tcgtcgagtt ggccacccgg cgcgacccgc acgacccggc
     4441 gaccgtcgcc tccctttcgg cggccgtcga cggctcgttg gagacgctgg cgctgttgcg
     4501 agccctcacc gaggccgacg cctgcgcggc gggtccggca gcgtggacgg attggcgggc
     4561 tcaactcgtc gcccggctga ccgcggtcgg cgcgcgggcc ctgcgcgagc ggcccgagcg
     4621 gcccgagcgg cccggtgcgg aggcggtcgg cgggatcggc gaaggcgggc tgggtgacga
     4681 cgggccgggt gccacggctg ccgagattgc tgaggtggcc gccgggcgac cggtcgtgcg
     4741 agtcgaccga cttggcaccg cacaccgcct gcacatcgtc gacaacgacc ggacgggcct
     4801 gttcgccgac accacggggc tgctcgcggc caccggcttc atcgtgcgtt cggcgctgct
     4861 gcggaccgtc gacggcatcg ccgtcaacga gtggcacgtc gactctccgt cgggggatgc
     4921 cccggatggg ggcatgctct cccgcgggtt gggtcgcttg gcgatgggcg accgatcacc
     4981 gttggcgcca ctggctcgac ggcggcgggc ccttgaggtg acagcgcatg cgtcgctcgg
     5041 ccgacccggg atgacgcggg ccatggtgct gcccgaggcc tcccgccagt cgacggtcat
     5101 cgaggtgcgc gccaccgacc gccgcgggct cctgcacgac ctcgggtccg cgatcaccga
     5161 cgccgggctc tccgtccgca gcgcccacat cgcgacgtat gccggtcagg cgctggacac
     5221 cttctacctc acccgcgccg acggctcacc gctggcgcct gcggaggtgg ggcgcaccat
     5281 cgccctgctc atcgacacca gcgacggtcg ggccgcgagt tgacgggcgc ctgagccgaa
     5341 cgttctcgcg aaggggcgcg ggcggcatac cggcaggctc gtatgccgcg cgaaaggtaa
     5401 ggttctgggg tgttcaacaa cctgtccgac cggctgaccg cgaccttccg caacctgcgc
     5461 ggcaaggggc gcctcaccga gcacgacatc aacgcgaccc tgcgcgacat ccggatggcg
     5521 ctgctcgacg ccgatgtcgc cctgcccgtc gtcaaggcgt tcgtcgcggc gatccgcgag
     5581 cgagcgctgg gcgccgaggt gagtcaggcc ctcaacccgg gccagcaggt cgtcaagatc
     5641 gtcaacgagg agctcgtcac catcctcggc gggcagaccc gcccgctcaa tctggccaag
     5701 aacccgccca cggtcatcat gctggccggt ctccaagggt ctggaaagac caccctcgca
     5761 ggcaaactgg gctactggct caaggagcag gggcacacgc cgatgctcgt ggccgccgac
     5821 ctgcagcggc ccaatgccgt cacccagctc gaagtggtcg ggcagcgcgc cggagtgccg
     5881 gtctatgccc ctgagcgcgg caacgtcgcc gggcacgacg cggtcggcgg cgccggtgag
     5941 ggcacccggt cctacggcga cccggtcaag gtcgccgacg acggcatcca gtacgccatc
     6001 gcccggcagc acgacgtcgt cattgtcgac accgctggcc ggctcgcggt cgacgccgac
     6061 ctcatggcgc aggccaagga catccgggcg gcgatcagtc ccgacgaggt cctcttcgtc
     6121 atcgacgcga tgatcggtca ggcggcggtc gaaaccgcgc aggccttcca agagggcgtc
     6181 ggcttcaccg gcgtcgtgct gtccaagctc gacggcgacg ctcgcggtgg tgccgcgctc
     6241 tcgatcgcgc acgtcactgg ccgcccgatc ctgttctcct cgatcggcga gggcgtcaag
     6301 gatttcgagg tcttccaccc cgaccggatg gcgtcgcgga tcctcgactt gggtgacgtc
     6361 ctcaccctca tcgagcaggc cgagcgggcg ttcgacaagc agcacgccga cgagatggcc
     6421 cgcaagttcc tcgccgaaga ggacttcacc ttcgacgact tcctcgagca gatggccgcg
     6481 atcaagcgga tgggcaactt caagtcgatg ctcgggatgc tgccgggcat gcagcagatg
     6541 cgggcgcagt tggacgcgtt cgacgagaag gagttcgacc gggtcgaggc catggtgcgc
     6601 tcgatgacgc cgttcgagcg aagccacccc aagaccatca acggctcccg gcgtcagcgc
     6661 atcgcccgcg ggtcgggcat gcaggtgtcc gatgtcaacc agttgctgga gcgcttcacc
     6721 caggctcaga agatgatgaa gtctttggct cggggcggcg gtccgggtct gccggggatg
     6781 cccgggatgc ctggcatgcg caagggcaag gccgcgcccc cgccgcgcaa gaaggccaag
     6841 agcggcaacc cggccaagcg cgcggctgag gagcgcgcgc tggctgatcg cgcgacgacg
     6901 ggtcgtcagg aggcggctgg acgagcgttc ggtatgccgc ccggcgcagg cggcgcttcg
     6961 ggtgccccgg gcgctgcgga tctcgacccc agcaagctcc ctccgggctt cgagaagttc
     7021 ctcggccgct gagccggcta agtgcctgcc ggctgggccg ctgaggtgct gatccgttga
     7081 ggccgtgagc cgttgaggtc ttgaggcctc aaggccttga gccgttgagg gcgtgacgga
     7141 gtgcgtttgc tgtggtgatc accccctcaa tcgcacaccg tcgggcactc ggcaggccgg
     7201 gctctatcgg actggcgaag ggagcggcga ggaccgtgac gctggctgag gtggacgggg
     7261 atccgcgcgt tatccacaac cctggcaatg ctggtcacgc catccacagg ttcaacggcc
     7321 cgggtgggca agggcagggc tggacgtcac gctggcggca tgacgctgga gcaggaagtc
     7381 gcaaccgggc agtggggcat cgtctcgcgc gcccaattgt cggctgccgg gatgagtccc
     7441 gaagcgatcc ggtggcgagc tgcccggcaa tggcgatcac ttctccccgg ggtttacgcg
     7501 atcgagccgg gccgtcttgg cgtgcaccag gcggccatgg ctgcgcagct ctatgtcggc
     7561 ccatctgggc tgctcggagg ctcctcggct gctcgactac atggggttga gagcgtgcgt
     7621 ctcaccgggc tcatcgacgt cgttgtcccg atgaacgggc gcacccgcac gatcggccac
     7681 gtcaggatcc cccgcaccag gttcagcgag ccggtgccgc accaccacgg aagcctgcgc
     7741 ctcatgtcgc cggctagggc tgtggccgaa gcgctgcgtc ggatgccgac cgatgaggtc
     7801 gccgccgagg tcgccatcga ggccgtgcaa cgacggatcg ctgcgctcga cgaccttcgc
     7861 cattggacgt cgctcctcgg gcgcgcagga tctgcccggg ctgaacgggc cctgtcggtt
     7921 gtcgcgtctg gcgcttggtc ggtccccgag caccgactcg cccatgcggt cactggggtc
     7981 aaagggctgc cgggcctatg gctgaacccc accctgaagg acgaacacgg cgcgcagctc
     8041 atcagccctg atctgtggtt cgacgaggtt gccttggcgg tgatggttca ctcgcgccgc
     8101 tatcactcga ccggcgacca atgggacgcc acagtcacct cggatgccga gctgagtgcc
     8161 gctggtgtgg tcgtcgtggg cgtcactcca caccagatca gcagagcgat gccggaggtg
     8221 ctggcccgga tcgcccgtgc gtatgccgcc gcgtctcgcc gaccccggcc gggcgtcaag
     8281 gcggtcccgc gtgggcgcga cggagtgcgt ctggtggggt gatcaccccg tcgatcgcac
     8341 tccgtcgggc acacaaacca cgccccccgg gcgagttagg ctcgtcggca tgacggcggc
     8401 aaccagcagg cggctgcaca ccctcgcggc gggcacatcc ggcccgcgaa tcgccttcct
     8461 gcacgggctc ttcggccaag gtcgcaactg gagccagatc gccaaggcgc tctcgggccc
     8521 agacggtgac caggcacagt gcctgctcgt tgacctgcct gatcacggcc actcaccgtg
     8581 gagtgaccac ttctccctcc aggcgtatgc cgcgtcagtc gccgcaacgc tccgcgcgac
     8641 cgccccgggc gaacgctgga ccctcgtcgg tcactccctc ggcggcaagg tagccatgat
     8701 cgtcgcgctg tcgcagcccg acctgatcga gcggctcgcg gtcatcgaca tcgcgcccag
     8761 gcactaccgc tcccaccagt ggttcgtcga ctacgtcaac gtcatgcgag cgctgccgct
     8821 tgactcgctg cccgaccggg catcggccaa tgctgcgctg gcgcaacaca tccccgaccc
     8881 ggcggtctgc gccttcctcc tgcagaacct gcgccgccac ggcaccgcat ggagctggca
     8941 gatcaatttc gacgtcctcg ctcgaccggg caccgggcca gcgggggtgg cgggctggcc
     9001 gaccgctgac gcgacggcat accagccctt ccgggggccg gtcgcctggg tggcgggtgc
     9061 ggagtcgacc tacatcaccg accgcgacgc cgagcccatg cgggcactgt ttcctcgggt
     9121 gacgcaggtg acggtcaagg acgccgggca ctgggtgcac tccgaccaac ccgaggtcgt
     9181 cacgcagatc ttgcgccgct tcgccctgtc gacggctcgc aggcactgac cgctggatac
     9241 agtcgcccca tggcgacggt gctgcacatc gtgggggaga tcctggtcgg acccgcggac
     9301 acgaggccgg agtgctgggt gctcgacggg cgggtgacgt atgccgcccc cactgggccc
     9361 ggtcacgacg tccagaccgt gcggggctgg gcgttacccg gcctcgtcga cgctcactgt
     9421 cacgtcggcc tggaccacca cggcccggtc gagcccgacg ttgccgagca gcaggccacg
     9481 gccgaccggg atgctggcac cctgctcatc cgtgactcgg gcagcccggc cgacacccgg
     9541 tggatcgacg accgggacga cctgccacga atcgtccggg ccggcaaaca catcgcccgg
     9601 acccggcgct acatccgcaa ctacgcccac gagatcgagc ccgacgaact cgtcgcgcac
     9661 gtccggatgg aggcgcgccg gggcgatggc tgggtcaagc tcgtgggtga ctggatcgat
     9721 cgggagaccg gcgacttggc gccctgctgg ccgcgcgagg cggtcatcgc cgcggtcgca
     9781 gccgcccacg aggagggcgc ccgcaccacc gctcactgct tcgccgagga gtccctgcac
     9841 gacttcgcgg cggcaggcac ggactgcatc gagcacgcca gcggcctgga cgaggagtcc
     9901 accaaagcct ttgcggcaca agggatcgcc atcgttccga cgctgatcaa catcgcgaac
     9961 ttccccgagt tggcggcacc ggcagcagag aagttcccgc gttatcacgc ccacatgctc
    10021 gacctgcacg cccgacggtt cgagacgatc gcgcgggcgc acgaggccgg catacccatc
    10081 tatgtcggaa ccgacgcggg tggggtgttg ccgcacggcc tcgccgctgc ggaggccctg
    10141 gagttggcca aggcggggtt gtcgacgctc gacgtcctcg gcgccggatc gtggaaggcc
    10201 cgcgagtggc tcggccacga agggatcgag gagggggcct cggccgactt cgttgtctat
    10261 cccgcggacc cgcgcgccga cctcgccgtc ctcggagccc ccgacgcaat catcctgcgc
    10321 ggcaaccgag ttcgatgatc cacgcgtggg agcggcggac tgacaaggtg cgcgtcgcac
    10381 ctctgacggc cgacggcctc cctgggcttc gccgcgccat ggctctctcg cagggtcgcg
    10441 tgggggtctg gggaccaacg ggattggact ccgtcgatga cctcctcgca gtccagggcc
    10501 gcagcaaacg caccttcatc gtccacgccc ttgccccgga ctccagcgca ggccacggca
    10561 tcgctgctct catcaacgtc aacgacatcg tcatgggacg gttcgcctcg gcaacgctcg
    10621 gttacgacgc ctacgacccg tatgccggcc gcggcctcac tcgggacggc ctggcgctcg
    10681 tgcttgacgt gtgtttcggg ccgccgccgg acggcctcgg actgcatcgc gtcgaggcgt
    10741 ccgtgcaacc cgccaacgtc cgctccgccg ggctgctgcg gtctcttggc ttcctgcgcg
    10801 aaggcttctc acgccgactg ctgcgactgc cgtcgctagg cgacgaggag gtcgactggc
    10861 gggaccacga ccgctacgcg ctgctctgcg aggactggcc gggcgccgcc gagagcgccc
    10921 gcgtcgccta tcgggctgcc ccgctcagac ccgtcgtgtg cgtcgtcaac gggctaccgg
    10981 gctcagggaa gagcaccctg gcaccccgtc tcgctgtcga gctcggagtt cccctcttgg
    11041 gcaaggacct catcaaggag gccgtggccg acgctctcga cgagagcgac caaggtcggt
    11101 tgagtaagga gctcggctcc ggagcgagcc gggcgctctg ggcattgctc gcagcctcgc
    11161 ccgcgggagg ggtcgtcgag acctggttgc cgccgacgcg cgacacggca tacctcgtgg
    11221 acggcctgcg cgctgcggga ctggacccga cccgggtccc tgaggtgttc tgcgacgtcc
    11281 cgccagaggt tgctcgcgag cgcgatctcg tgcgcgccgc gaggggagag cggcaccccg
    11341 tccaccgtgg tccggtcgag gagacggagt ggcacgagtc ggtc
//
LOCUS       DAOMZY010000072        11295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5386_length_11295_cov_6.604448, whole genome shotgun sequence.
ACCESSION   DAOMZY010000072 DAOMZY010000000
VERSION     DAOMZY010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 11295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11295
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5386_length_11295_cov_6.604448"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..1066)
                     /locus_tag="PLK64_05760"
     CDS             complement(11..1066)
                     /locus_tag="PLK64_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV79280.1"
                     /translation="MTVAPGEPENRSDASAVPVASAVAVDPGSPVALDGVDGAVAIAG
                     RTPWQIVMRRLRRDRVTMVAFGASVFFVALAAIAPLLARIGILNPDQTHPELVQGLGS
                     MPEGLMGGVSWSHWLGVEPGTGRDLLSRIVTGMTTSLTVALLATLFSVVVGTIIGIIS
                     GFAGGRVDWLLSRSMDLVLSFPSTLMLLSLQAVLVDRIASIMGQPSSAPPPKIAFMVL
                     VLGFFGFPFFARIIRGQVLSLREREFVEAARSLGARRGRLYFKELLPHLWAPLLVYTT
                     LVLPANISAEAALGFLGVGLNPPTASLGAILNDTVSYALPDPAYFFFPGVTVFLLVLS
                     FNLLGDGLRDALDPKDR"
     gene            complement(1205..>2507)
                     /locus_tag="PLK64_05765"
     CDS             complement(1205..>2507)
                     /locus_tag="PLK64_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPV79281.1"
                     /translation="TSTATATDIVESGSGSAEFDAAVSCRGATLTMRLKVPVPDFPEI
                     VALPAFAAYRRDHDRGGAGTFDVFSCGPYRLDGAWEAGAGGRFVRNPSWSRAGDALRQ
                     ALPDVVDIRESIPVNTLVQRLVEDKAPDNTAIGITDIPAAGHAALLADPAVKARLSNP
                     LSGTVELLQPNVKSPVMSNEGVRRALALATDRAAFVAAYGSTVMTPAYSALASSIPGH
                     PATPPAADATPTATASATANSIAPLPTAADPDAARAALVAAGVTLPVHIRVAYRTSPA
                     ADAAYAALEAGWEKVGFEVTLDGLGEDYYRIVSGIDASANYDVFRRSWFADYPAGAAV
                     IPDLFDGRMNLTDSGTGQDLGSFNDEAVNTAIADAQVEADPVKRALAWAAIDAKIVAV
                     VGQIPLAERRRSFLRGSGVGGYAENPYLGGWVDLAGVSVAH"
     assembly_gap    2508..2607
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2608..3245)
                     /locus_tag="PLK64_05770"
     CDS             complement(<2608..3245)
                     /locus_tag="PLK64_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPV79282.1"
                     /translation="MRGSGSPRYAARAAYLFLASVLLTGACTGSPNSPSPTEAAGGTL
                     RLLYDQTITTWDPQRMYSGPEGAIAVRLFSRTLTSYPAGGAGGVGGLVGDLATDTGTP
                     SDGGKTWTFTLRSGPAWEDGKPVTCQDVAYGIARSFAREQLPGGAPYPMTLLDIPSRV
                     DSAGLEVPGYPGPYAVTPPPAPTAEPTSTGTAQATATATESALPTASETSTAT"
     gene            complement(3251..5179)
                     /gene="typA"
                     /locus_tag="PLK64_05775"
     CDS             complement(3251..5179)
                     /gene="typA"
                     /locus_tag="PLK64_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HPV79283.1"
                     /translation="MPTQHRGDIRNVAIVAHVDHGKTTLVDKMLWQGGAFGAHQHVEE
                     RAMDSGDLEREKGITILAKNTAIHYNGPSAVAAGHAKGVTINIIDTPGHADFGGEVER
                     GLSMVDGVVLLVDASEGPLPQTRFVLRKALTAHLPVVLCINKVDRPDARIAEVVDEVY
                     ELFMDLLEGSDDERHHDHALEFPIVYASAKAGRASLERPTDGGLPDGPDLEPLFKTIM
                     ETVPAPVYDDEAPLQAHVTNLDSSNFLGRLALLRVHNGTMRKGQQVAWCRRDGSVQRV
                     KITELLMTEGLERKPMDPDTAAGPGDIIAVAGIDEIMIGETLADLDDPRPLPLITVDE
                     PAISMTIGTNSSPMAGRARGTKVTARLVKDRIDRELVGNVSIRVLNTDRPDTWEVQGR
                     GELALAILVEQMRREGFELTVGKPQVVTKQVDGKVHEPMERLTIDTPEEFLGVVTQLM
                     AARKGQMEHMTNHGTGWVRMEFLVPSRGLIGFRTEFLTETRGTGIAHHVFEGYAPWAG
                     PITTRINGSLVADRSGAVTAYAMVNLQERGTLFVEPTTEVYEGMIVGENSRADDMDVN
                     ITKEKKLTNIRASSSDNFEKIVPARKLSLEQSLEFCREDECVEVTPDAVRIRKVVLDQ
                     VERGRTASRARSAAKAEN"
     gene            complement(5193..5633)
                     /locus_tag="PLK64_05780"
     CDS             complement(5193..5633)
                     /locus_tag="PLK64_05780"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HPV79284.1"
                     /translation="MTSSTAVVAAAIVDSLDCPSRLLAARRTYPVEVAGRWELPGGKV
                     EPGETATDALHRELAEELGIHLTLGGQIVGPLAGGAWPLTPAHHLLVWLAQVREGGIP
                     TPLQDHDEVRWLTADTLYAVPWLPGDLPVVEALPPLMVGEPLNT"
     gene            complement(5635..6120)
                     /locus_tag="PLK64_05785"
     CDS             complement(5635..6120)
                     /locus_tag="PLK64_05785"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPV79285.1"
                     /translation="MARFNWEALPTRWQRADLDDPLNVVILLRQALLGAERAVHEVTD
                     GLLREAVGPGDEIALLHILRRVRGRETVVAQLADFLRCTHPVISRRVKRLEDAGLVLR
                     SGSWRDARSSSLTLTAAGSEFVDDLNSALGELAQLWGDEVETDRWVVLMKALDALADA
                     A"
     gene            complement(6369..7379)
                     /gene="argF"
                     /locus_tag="PLK64_05790"
     CDS             complement(6369..7379)
                     /gene="argF"
                     /locus_tag="PLK64_05790"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPV79286.1"
                     /translation="MAVDLTNRHFVKEIDFTPTEWMGLVGLTRSLKAARREGREERML
                     VGKTIALIFEKTSTRTRCAFEVAAYHLGAQLTYLDPHGSQIGHKESFADTGRVLGRLY
                     DAIEFRGSRHEDCETLARYAGVPVYNGLTDMWHPTQALCDIVTMVEHSPKPAAEISYA
                     YVGDARYNMGNSLLVAGAMSGMDVRIVAPRQLWPSVEIVGHARRLAETTGARIALTED
                     LDEGVAGVDFVHTDVWVSMGEGDAVWGERIELLTPYQVTSTVMASTGNPTTKFMHCLP
                     SFHNRETAVGEKIFAEFGLEAMEVTDEVFESPASIVFDQSENRMHTIKAVLAATLVGH
                     QL"
     assembly_gap    7447..7546
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7582..8022)
                     /locus_tag="PLK64_05795"
     CDS             complement(7582..8022)
                     /locus_tag="PLK64_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV79287.1"
                     /translation="MNPAHLQVDRVHAWLSQEAYWALGRTRETVEKAIAGSRTMGVYR
                     DRGDGMPGEQVAFARAVTDGATFAWVCDVFVDSDVRGLGVGNAMVGQLVTALEDLGIG
                     RVLLATRDAHEVYARLGFEPLANPTFWMERDRRPQRFVPGTASG"
     gene            complement(8048..8248)
                     /locus_tag="PLK64_05800"
     CDS             complement(8048..8248)
                     /locus_tag="PLK64_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HPV79288.1"
                     /translation="MAGKFEVYQDSAGKFRFRLKATNGQVIAVGQAYETKKSCLNGID
                     SIRKNAPDAKLDDQTAAPAAKA"
     gene            complement(8352..9671)
                     /locus_tag="PLK64_05805"
     CDS             complement(8352..9671)
                     /locus_tag="PLK64_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HPV79289.1"
                     /translation="MTSPATVVVLGAAGRDFHNVNVVFRDDPAYRVVAFTATQIPDID
                     DRRYPPELAGPLYPDGIPIVPETDVVELIEREGIDLAVFAYSDVPHSRVMHLASQCLA
                     AGADFTMLSGRHTMLTSSKPVVAVTAVRTGVGKSQTTRYLAKLLREKGLRVVAVRHPM
                     PYGDLAAQAVQRFAAYADLDRYECTIEEREEYEPHIDAGCVVYAGVDYERILRQAETE
                     ADVILWDGGNNDLPFYRPDLHVCLVDPLRPGDERRYHPGEANVRMADLVIVNKCDAAR
                     PEDIDAVEASVREMNPLAQIHRCDSPVTVEGGAAVEGKDVIVVEDGPTLTHGSMTFGA
                     GVVGASAARVSRIVDPSPYAVGSLAATYAKYPNACGVLPAMGYSSEQIRDLEATIEAT
                     PADAIVSGTPIDLTRVLTVSKPIVRASYELREREPGRLRAALDAVLG"
     gene            complement(9795..>11295)
                     /locus_tag="PLK64_05810"
     CDS             complement(9795..>11295)
                     /locus_tag="PLK64_05810"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV79290.1"
                     /translation="TPAPPGFTPQTSGPQMSGAERAVFAFGDVFGGGAAATLAVLYLF
                     YLTDIVGLPPGLAGTTVVVSKVWDAINHPLMGIITDRTRTRWGRRRPWIFAGALLLPP
                     AMAGLWAPIGDWDSQSAKVWFVILAHLFWTTVASIVAVPYGSLSTEITTDPDERSRVN
                     VLRLGCATFSAVGCTLVMNALVNAFTEGHLSMTTLYLSLFLGFGTFFAVPLLFVARFT
                     HERAPVAEHPDAFNLKVVISPFRIASFRRLTAMYLCPSITIDIVTSVILYYALYAVPG
                     VRTVLFAGIFAVVTLVMLPVLLRLVDRVDKNVIYYTGIPLSILAMVAIAFYPSGTIVW
                     PVYVLAGIFAIGLAAAQVMIWVMFPDVVDDGEIAQGARNAGSFSGVMVLVRTMASALA
                     TFAVGWVLEFTGYQHARHGEARLAQPESALWGIRLTMLITVVVLMGLAWFLARGYTLN
                     RARVLQLSVDLERARAARHTVAPEHAAHHGAHEPGEHEAGEHEPDQPHI"
BASE COUNT         1834 a         3611 c         3943 g         1707 t
ORIGIN      
        1 acgggagcca tcaacgatcc ttggggtcga gcgcgtcgcg gaggccgtcg ccgagaaggt
       61 tgaaggacaa cacgaggagg aacacggtca cccccgggaa gaagaagtag gccgggtcgg
      121 gcagcgcata agagaccgtg tcattgagga tcgcgccgag cgaggcagtc ggcgggttga
      181 ggccgacgcc gaggaagccc agggccgctt cggccgagat gttggccggg aggacgagcg
      241 tggtgtagac gagcaggggg gcccacaggt gcgggagcaa ctccttgaag tagagccggc
      301 cgcggcgtgc cccgagcgag cgggccgcct caacgaactc gcgttcacgc aacgacagca
      361 cctggccgcg gatgatccgc gcgaagaacg ggaagccgaa gaagccgagg acgagcacca
      421 tgaacgcgat cttcggtggt ggagcgctcg acggctgacc catgattgag gcgatccggt
      481 cgacgagcac cgcctgcaac gagagcagca tgagggtgct ggggaaggac agcacgaggt
      541 ccatcgaccg gctgagcagc cagtccacgc ggcctccggc gaagccggag atgatgccga
      601 tgatcgttcc gacgacgacc gagaagagcg tggcgagcag tgccacggtg agcgacgtcg
      661 tcataccggt gacgatgcgc gagagcaggt ctcgcccggt gccgggttcc actccgagcc
      721 agtgcgacca cgacacgccg cccatcaggc cttcgggcat cgagccgagg ccttgaacga
      781 gctcggggtg ggtctggtcg gggttgagga tgccgatcct ggcgagcagc ggggcgatcg
      841 cagcgagcgc gacgaagaag acggaggcgc caaacgccac catggtcacc cggtcacggc
      901 gcaacctgcg catgacgatc tgccagggcg tccgcccggc gatagcgact gcgccgtcca
      961 ccccgtcgag cgcgacgggg gacccgggat caacggccac ggccgaagcc acgggcaccg
     1021 ctgacgcgtc cgaacggttc tcgggttcgc ccggggccac agtcatcctc aggtcgtcct
     1081 tccgtcgcgt tcggccatcc gcaggtgatc cttcaggttt gggacaccct agccggggcc
     1141 caacccaaca aaggaacaac ttgaacggag attctccgga tcgtgaccga accgcgatcg
     1201 ttcgtcagtg ggcgacggac acccccgcca ggtcgaccca gccgcccagg taggggttct
     1261 ccgcgtagcc cccgaccccg gacccgcgca ggaacgaacg gcgtcgctcg gccaacggga
     1321 tctgcccgac gaccgcgacg atcttcgcgt caatcgcggc ccacgccaag gcccgtttca
     1381 cggggtcggc ctcgacctgg gcgtccgcga tggcggtgtt gaccgcctcg tcgttgaagg
     1441 accccaggtc ctgcccggtc ccgctgtcgg tgaggttcat ccgcccgtcg aaaaggtcgg
     1501 gaatgaccgc tgccccggcc gggtagtcgg cgaaccagga cctgcggaac acgtcgtagt
     1561 tggccgaggc gtcaatcccc gacacgatcc ggtagtagtc ctcccccagc ccgtccaacg
     1621 tgacctcgaa gcccaccttc tcccacccgg cctcaagcgc ggcatacgcg gcatccgcag
     1681 caggggacgt gcgataggcg acccggatgt gcacgggcag ggtgacgccg gcagcgacga
     1741 gcgcagcccg cgcagcatcg gggtcggccg ccgtcgggag cggagcaatg gagttcgccg
     1801 tggcagaggc cgtggcggtc ggcgtggcgt cggcggctgg gggcgtcgcc gggtgccccg
     1861 gaatggaact ggcgagcgca gagtaggcgg gcgtcatcac cgtgctcccg tatgccgcga
     1921 cgaacgcggc tcggtcggtc gccagggcca gcgcccgtcg gaccccctcg ttgctcatca
     1981 cggggctctt gacgttgggc tgcagcaact ccaccgtgcc cgacaacgga ttggagagcc
     2041 gggccttcac cgctgggtcg gcgagcaatg cggcgtgtcc ggcggccgga atgtcagtga
     2101 taccgatcgc cgtgttgtcg ggagccttgt cctcgacgag ccgctggacc agcgtgttga
     2161 cggggatcga ttcgcggatg tcgaccacgt cggggagcgc ttggcgcaac gcatctcccg
     2221 cgcggctcca cgaggggttg cgaacgaacc gtccacccgc ccccgcctcc cacgcgccgt
     2281 ccaatcggta agggccgcag gagaatacgt cgaaagtccc tgccccgccg cggtcgtggt
     2341 cccgacggta tgccgcgaac gccggcaacg cgacgatctc ggggaagtca gggaccggca
     2401 ccttgaggcg catggtgagg gtcgcgcccc gacaggagac tgcagcatcg aattcggccg
     2461 acccgctgcc ggactcgacg atgtcggtcg ccgtcgctgt cgaggtcnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnngtc gctgtcgagg tctcgctcgc cgtcggcagg
     2641 gccgattccg tggcggtcgc cgtggcctgc gccgtgccgg tgctcgtggg ctcagccgtg
     2701 ggagcgggcg gcggggtgac ggcatacgga ccgggatagc caggcacctc gagaccggcg
     2761 gagtccaccc ggctggggat gtcgaggagc gtcattgggt agggggcgcc gccgggcagt
     2821 tgctcgcgcg cgaaagaccg cgcgatgccg taggcgacgt cctggcaggt gaccggtttc
     2881 ccgtcctccc acgccggccc cgagcgcagg gtgaacgtcc acgtcttccc gccgtcggac
     2941 ggggtgccgg tgtcggtcgc gaggtcgccg acgagcccac cgactccacc ggccccaccg
     3001 gccgggtagc tcgtcagggt cctgctgaac aggcgcaccg cgatggcgcc ctcgggaccg
     3061 gagtacatcc gctgcgggtc ccacgtggtg atcgtctggt cgtagagcag tcgcaacgtg
     3121 ccgcccgcgg cctccgtcgg ggacggcgag ttcggggagc ccgtgcacgc gccggtgaga
     3181 agcacagacg caaggaacag gtatgccgcg cgcgcggcat acctgggcga gcctgaccct
     3241 cgcatgaggg ctagttttcg gccttggcgg ctgaacgggc acgggaggcc gtgcggccac
     3301 gttcgacctg gtcgaggacg accttgcgga tgcggacggc atcgggggtg acctcgacgc
     3361 actcgtcctc gcggcagaac tcgagggact gctccaggct caacttgcgg gccgggacga
     3421 tcttctcgaa gttgtccgag ctggaggccc ggatgttcgt cagcttcttt tccttcgtga
     3481 tgttgacgtc catgtcgtcg gcgcgggagt tctcaccgac gatcatgcct tcgtagacct
     3541 cggtggtcgg ctcgacgaag agcgtcccgc gctcctgcag gttgaccatc gcgtaagccg
     3601 tgaccgcacc agagcggtcg gcgacgagcg agccgttgat ccgggtcgtg atcgggccgg
     3661 cccagggcgc gtagccctcg aacacgtggt gggcgatgcc ggtgccacgg gtctcggtga
     3721 ggaactcggt ccggaaaccg atgagcccgc gggaggggac gaggaactcc atccgcaccc
     3781 agccggtgcc gtggttggtc atgtgctcca tctggccctt gcgggcggcc atgagctggg
     3841 tgaccacgcc gaggaactcc tcgggggtgt cgatggtgag gcgctccatc ggctcgtgaa
     3901 ccttgccgtc gacctgcttg gtgacgacct gaggcttgcc aacggtgagc tcgaagcctt
     3961 cgcggcgcat ctgctcgacg aggatggcga gggcgagctc gccacgcccc tggacctccc
     4021 aggtgtccgg gcggtcggtg ttgaggaccc ggatggagac attgccgacg agctcgcgat
     4081 cgatgcggtc cttgaccagc ctggcggtga ccttggtgcc gcgggcgcgc ccggccatgg
     4141 gcgagctatt ggtgccgatg gtcatcgaga tggccggctc atcgacggtg atgaggggca
     4201 gcgggcgcgg gtcgtcgagg tcggccaagg tctcgccgat catgatctcg tcgatgccgg
     4261 cgacggcgat gatgtcgccg ggaccggccg ctgtgtcggg gtccatcggc ttgcgctcca
     4321 ggccctcggt catcagcaac tcggtgatct tgacccgctg gacactgccg tcgcgccgac
     4381 accaggcaac ctgctggccc ttgcgcatcg tgccgttgtg gacccgcagc agcgccaggc
     4441 ggccgaggaa gtttgacgag tcgaggttgg tgacgtgcgc ctgcagcggt gcctcgtcgt
     4501 cgtagacggg tgcgggcacc gtctccatga tcgtcttgaa gagcggctcg aggtcagggc
     4561 cgtcgggcag cccgccgtcg gtggggcgct ccaacgaggc ccggccggcc ttcgccgagg
     4621 catagacgat ggggaactcc agcgcgtggt cgtggtgacg ctcgtcgtcg gaaccctcga
     4681 gcaggtccat gaacagctcg tagacctcgt cgacgacctc ggcgatccga gcgtcaggcc
     4741 ggtccacctt gttaatgcac aggacgaccg ggaggtgagc ggtcagcgct ttgcgcagga
     4801 cgaaccgggt ctgcggcagg gggccctcgg acgcatcgac gagcaggacg acaccgtcga
     4861 ccatcgacag gccccgctcg acctcaccgc cgaaatcggc gtggccaggg gtgtcgatga
     4921 tgttgatcgt cacgcccttc gcgtgccccg ctgcaacggc cgacggcccg ttgtagtgga
     4981 tcgccgtgtt cttggcgagg atcgtgatgc ccttctcgcg ttcgaggtca ccggagtcca
     5041 tcgctcgctc ttcgacatgc tggtgggcgc cgaaggcgcc cccctgccag agcattttgt
     5101 cgacgagcgt ggtcttaccg tggtcgacgt gggcgacgat ggcgacgttt ctgatgtcgc
     5161 cgcggtgctg cgtgggcata aggatcagtc tctcaggtat tcagaggctc accgaccatc
     5221 agggggggca gggcctccac aacggggaga tcgccgggga gccagggcac ggcatacagg
     5281 gtgtcggcgg tcagccagcg cacctcgtcg tggtcctgca acggcgtcgg tatgccgccc
     5341 tcccgcacct gcgccagcca cacgagcagg tggtgagcgg gggtgagcgg ccaggcgccg
     5401 cctgcgagtg gcccgacgat ttggccgccc aacgtgaggt ggatgccgag ctcttcggcg
     5461 agttcacgat gcagggcgtc ggtcgcggtc tcaccgggct cgactttgcc gccgggaagc
     5521 tcccatcggc cggcgacctc gaccgggtag gtgcgcctgg cggccagcag gcgggagggg
     5581 cagtcgagtg agtccacgat cgctgcggcc acgaccgccg tcgagctggt catctcaggc
     5641 cgcatcggcc aaggcgtcca gcgccttcat cagcacgacc cacctgtcgg tctcgacctc
     5701 gtcgccccac agctgcgcca gctccccgag cgcgctgttc aagtcgtcga cgaactcact
     5761 tccggcggct gtcagcgtga gggacgacga ccgggcgtct cgccacgaac cggatcgcag
     5821 gaccagcccc gcgtcctcca accgcttgac ccgtcggctg atgaccgggt gggtgcagcg
     5881 aaggaagtcc gccaactgtg cgacgacggt ttctcgaccc ctgacgcggc gcaggatgtg
     5941 gaggagggcg atctcgtcgc ctgggccgac ggcctcgcgg aggaggccgt cagtcacctc
     6001 gtgaaccgcg cgctcggcac ccaacagtgc ctggcgaagc aggatgacga cgttgagcgg
     6061 gtcgtcgagg tctgctcgct gccagcgggt gggcagggcc tcccagttga atcgcgccat
     6121 gcccctatcg aagcgcactg tcactgaaga cgccgggcgc tatccacagg ggccgaaagg
     6181 cgtcggcgct gccacagcca ggggacatac ccccgtgaaa ttccccgtac ctcaggcacg
     6241 cactgcgtac ttcaggtacg cggaaatttc agccctcctt gggggcggcg cggaagggtg
     6301 agatgtccgc gagagcagtg gttccgggag agcaggggct ccaggaggga aggggctccg
     6361 cctggtggtc agagctgatg acccaccagg gtcgcggcga ggaccgcctt gatggtgtgc
     6421 atccggttct cgctttggtc gaagacgatc gacgccggcg actcgaacac ctcgtcggtc
     6481 acctccatcg cctcaagacc gaactcagcg aagattttct cccccaccgc cgtctcgcgg
     6541 ttgtggaacg agggcaggca gtgcatgaac ttcgtggtcg ggtttccggt ggacgccatg
     6601 acggtcgagg tcacttggta aggcgtgagg agttcgatcc gctcgcccca caccgcgtca
     6661 ccctcaccca tcgacaccca cacgtcggtg tgcacgaagt cgactccggc aacgccttcg
     6721 tcgaggtcct cggtcaacgc gatccgggca cccgtggtct cggcgagtcg ccgggcgtgt
     6781 ccgacgatct cgacgctggg ccacaactgc cggggggcga cgatccgcac gtccatcccc
     6841 gacatggcgc cggcgacgag caatgagttc cccatgttgt agcgggcatc cccgacgtag
     6901 gcgtaggaga tctcggcggc cggcttgggc gagtgctcga ccatggtgac gatgtcgcac
     6961 agcgcctgag tcgggtgcca catgtcggtt agcccgttgt agacgggcac accggcatac
     7021 ctcgccaggg tctcgcaatc ctcgtgccgt gaccctcgga actcgatggc gtcgtagagc
     7081 cggccgagca cccgaccggt gtcggcgaac gactccttgt gcccgatctg gctgccgtgc
     7141 gggtcgaggt aggtgagctg ggcacccagg tggtaggccg ccacctcgaa ggcacatcgg
     7201 gtccgggtcg aggtcttctc gaagatcaac gcgatcgtct tgccgacgag catccgctcc
     7261 tcgcgacctt cgcggcgggc agctttgagg ctgcgagtca gccccacgag ccccatccac
     7321 tcggtggggg tgaagtcgat ctccttgacg aagtgccggt tggtgaggtc cacagccatg
     7381 cgccaaccgt atgccgtccg ctcgccaccg cggcgtcgct gcccacgcga ggtggatcac
     7441 ccggggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtgg atcagccggg
     7561 ggtagatcag ccgggaccgg gtcaaccgga ggcagtccct ggcacgaacc gctgcggtcg
     7621 acggtcgcgt tccatccaga acgtcggatt ggccagcggc tcgaaaccga gccgggcgta
     7681 gacctcgtga gcgtcgcggg tggccaacaa gacccgtccg atccccaggt cctccagcgc
     7741 cgtcaccaac tggcccacca tcgcgttgcc caccccgagg cctcgcacgt cggagtcgac
     7801 gaacacatcg cacacccacg cgaaggtggc cccgtcggtg acggcgcggg cgaacgcgac
     7861 ctgctcaccc ggcataccgt cgccgcggtc gcggtagaca cccatggtgc gcgaccccgc
     7921 gatcgccttc tcgacggtct ctcgcgtgcg cccgagggcc caataggcct cttgcgacaa
     7981 ccacgcatga acccggtcca cctgcaggtg ggccgggttc atgctgaaga cgaagccgtc
     8041 caggacgtca ggccttggca gcgggagccg ccgtctggtc gtcgagcttg gcatccgggg
     8101 cgttcttgcg gatcgagtcg atgccgttga ggcagctctt cttcgtctcg taggcctgac
     8161 ccacggcgat gacctgaccg ttggtggcct tgaggcggaa gcggaacttc ccggcactgt
     8221 cctggtagac ctcgaacttg cctgccatgt gaacctccca ctcacgagtg acattgacat
     8281 tcgcagcgta ggacgcgcca ctcgttccgg cgcgcccatc ggacacgcca cgccgactcg
     8341 gcggcctcgg gtcagcccaa caccgcgtcc aacgccgccc gcagtcgccc gggctcgcgc
     8401 tcgcgcaact cgtaactggc ccggacgatg ggcttgctga cggtgaggac ccgggtgagg
     8461 tcgatgggcg tgccgctgac gatggcgtcc gccggcgttg cttcgatggt ggcctcaagg
     8521 tcgcgaatct gctcggacga gtagcccatt gcgggcagga ccccgcacgc gttggggtac
     8581 ttcgcgtagg tcgcggccag gctcccgacg gcatacgggg aggggtcgac gatgcgcgag
     8641 acgcgggccg cgctcgcccc gaccactccg gcgccgaaag tcatcgagcc gtgagtgagg
     8701 gtcggcccgt cctcgacgac gatgacgtcc ttgccttcga cggccgcgcc accctcgacg
     8761 gtgacggggc tgtcgcaccg gtggatctgg gccaacggat tcatctcgcg gaccgacgcc
     8821 tcgacggcgt cgatgtcctc gggccgagcc gcgtcgcact tgttgacgat gaccaggtcg
     8881 gccatgcgga cgttggcctc gcccgggtgg tagcggcgct cgtcaccagg gcgcagtggg
     8941 tcgacgaggc agacgtgcag gtccggccgg tagaacggga ggtcgttgtt gccgccgtcc
     9001 cagaggatga cgtcggcctc cgtctcggcc tggcgcagga tccgctcgta gtcaacgccg
     9061 gcatagacga cgcagcccgc gtcgatgtgg ggttcgtact cctcgcgctc ctcgatggtg
     9121 cactcgtagc ggtcgaggtc ggcataggcg gcgaaccgct ggacggcctg cgcggccaga
     9181 tcgccatagg gcatcgggtg gcgaacggcg acgacacgca aacccttctc gcgcaacaac
     9241 ttcgccagat agcgggtggt ctggctcttg ccgacccccg tgcgcaccgc ggtcacggcg
     9301 acgaccggct tgctggaggt gagcatggtg tgacgaccgc tgagcatggt gaagtcggca
     9361 cccgcggcca ggcactggga cgccagatgc atgacccggc tgtgcgggac gtcggagtag
     9421 gcgaagaccg ccaggtcgat tccttcgcgc tcgatgagct cgacgacgtc ggtctcggga
     9481 acgatcggga tcccgtcggg atagagcggg ccggccaact ccgggggata acgccggtcg
     9541 tcgatgtcag ggatctgggt ggcggtgaag gcgactacgc gatacgccgg atcgtcgcga
     9601 aacacgacgt tgacgttgtg gaagtcacga cccgctgcgc cgaggacgac taccgtggca
     9661 gggcttgtca taacgcgctc cgtggctggg tcgaacaccg gcgccgggat gccctcgtgg
     9721 cgccccgacc gtgggactcg ccgtaatcca ctgtatgagc ggctgccgcg agggcaggga
     9781 ccttcggccc gtcctcagat gtggggttgg tcaggctcgt gctcgccggc ctcgtgttcc
     9841 cccggctcat gcgcaccgtg gtgcgcggca tgttcgggag cgacggtatg ccgtgccgcc
     9901 ctggcccgct ccaggtcgac gctaagttgc agcacccggg cccgattgag ggtgtaaccc
     9961 cgagccagga accaggccag gcccatgagg acgaccacgg tgatgagcat ggtcaaccgg
    10021 atcccccaga gcgcactctc gggctgggcg agccgggcct cgccgtgccg ggcatgttga
    10081 tagcccgtga actccagcac ccagccgacc gcgaaggtcg cgagcgccga ggccatagtg
    10141 cggacgagga ccatcacccc gctgaacgat ccggcgttgc gggccccttg agcgatctca
    10201 ccgtcgtcga cgacgtccgg gaacatcacc cagatcatca cctgagccgc ggccaagccg
    10261 atggcgaaga tccctgccaa gacgtagacc ggccagacga tggtgcccga cgggtagaag
    10321 gcgatggcca ccatcgcgag gatcgagagc gggatcccgg tgtagtagat gacgttcttg
    10381 tcgacccggt ccacgaggcg caggagcacc gggagcatga cgagggtcac cacggcgaag
    10441 atcccggcga agaggacggt gcgaactccc ggcacggcat acagggcgta gtagaggatc
    10501 accgaggtga cgatgtcgat ggtgatcgac gggcagaggt acatcgcggt gagccgccgg
    10561 aaggaggcga tccggaacgg tgagatgacg accttgagat tgaacgcgtc tgggtgctcc
    10621 gcgaccggtg cccgttcgtg ggtgaacctg gcgacgaaca gcagtggcac cgcgaagaag
    10681 gtgccgaagc cgaggaacaa cgacaggtag agcgtcgtca tcgacaggtg gccctcggtg
    10741 aacgcgttga cgagggcgtt catcacgagg gtgcagccga cggccgagaa ggtggcgcag
    10801 ccaaggcgca ggacgttgac ccggctgcgc tcgtccgggt cggtggtgat ctcggtcgac
    10861 agcgatccgt agggaacggc gacgatgctc gccacggtgg tccagaacag gtgggcgagg
    10921 atgacgaacc acaccttggc gctctggctg tcccaatcgc cgatcggcgc ccagaggccg
    10981 gccattgccg gtggcagcaa caacgccccg gcgaagatcc aagggcggcg ccggccccag
    11041 cgggtgcggg tgcggtcggt gatgatgccc atgagcgggt ggttgatcgc gtcccagacc
    11101 ttggagacga cgacggtcgt gccggccagg ccggggggca acccgacgat gtcggtgaga
    11161 tagaagaggt agaggaccgc gagagtcgcc gcggcccctc ccccgaagac gtcaccgaag
    11221 gcgaagaccg cccgctcggc cccggacatc tgcggcccgg acgtctgggg ggtgaacccg
    11281 ggcggtgcgg gggtg
//
LOCUS       DAOMZY010000073        11292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5389_length_11292_cov_5.837234, whole genome shotgun sequence.
ACCESSION   DAOMZY010000073 DAOMZY010000000
VERSION     DAOMZY010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 11292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11292
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5389_length_11292_cov_5.837234"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..706)
                     /locus_tag="PLK64_05815"
     CDS             complement(26..706)
                     /locus_tag="PLK64_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002858071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPV79291.1"
                     /translation="MSRILIVEDEESFSDPLSYLLEKEGFAVAVAATGPDALAEFDAN
                     GADLVLLDLMLPGLSGVEVCRALRAKSSVPIIMLTAKDSEIDKVVGLEMGADDYVTKP
                     YSARELLARVRAVLRRLAEADEPADATVEAGPVRMDVDRHVVTVKGKATPMPLKEFEL
                     LELLLRHAGRVLTRGQLIDRVWGSDYVGDTKTLDVHVKRLRAKIEPDPGNPRHILTVR
                     GLGYKFEA"
     gene            complement(703..2028)
                     /locus_tag="PLK64_05820"
     CDS             complement(703..2028)
                     /locus_tag="PLK64_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPV79292.1"
                     /translation="MEVLLAVILGAVFGAVVVSARFGRTRVDRRRALAAGATPDPEEA
                     VEQLPAEVTDLLAALTSSGIVLDSRQSVVACSPTAISHGLVRGTAVSHPPLLALARRA
                     AIEGGVVEEMLGLPRGPRADARRVVQARATHLGLEHILLLVEDRSHAYLVEEVRRDFL
                     ANVSHELKTPVGGLMLLAEAVRDARDDPEAVARFAKRMRKETRRLDQLVSDIVQLSRL
                     QSEDRLSDPDAVDLGGVVRQAVDDVRITAEERRIDLVVRCDANCWVWGDAGLLTTAVR
                     NLVVNAVAYSASGTRVAVRALIAPDGAREIIVSDQGQGIPESELGRIFERFYRIDAAR
                     SRATGGTGLGLSIVKHVCANHGGDVTVWSEVGRGSTFTIRLPAPAYGPDDQSTTGSIG
                     VLAPGDVRGGEGGPAASTADPASPDHHLDGALASTGPQSSDVTREVRPA"
     gene            2221..2934
                     /gene="phoU"
                     /locus_tag="PLK64_05825"
     CDS             2221..2934
                     /gene="phoU"
                     /locus_tag="PLK64_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HPV79293.1"
                     /translation="MRDAFHEQLDSITAQLVDMTRLAGSAMARATTALLDADLHLAED
                     VITGDRTLDNAREHLDSLAIDVLARQQPVATDLRTVVTALRMSGDLERMGDLARHVAK
                     VARLRFPNSAVPPSLRAHILQMGQVAELVVTKSGSIIAARDVTEARNLERDDDVMDEL
                     HRRLFTVLATTELPTETIVDLTLLGRYYERFADHAVSVARRVVYLVTGEYVPETSGDH
                     DVSEEGARIALLRRSSPRG"
     gene            3044..3388
                     /locus_tag="PLK64_05830"
     CDS             3044..3388
                     /locus_tag="PLK64_05830"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPV79294.1"
                     /translation="MAKRAIVAQPEVLDAARTLGVQIRIARHERGWTAADLASKIGVS
                     AKTVLAIEAGAVSTSIGTVFNAAVMVGVPLFGVEDRFEMARMRRRGEERLALIGQRVR
                     VPKLDETDLANF"
     gene            3375..4679
                     /locus_tag="PLK64_05835"
     CDS             3375..4679
                     /locus_tag="PLK64_05835"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HPV79295.1"
                     /translation="MPTSKPPQSLFVWTWLPGDTTPVVAGRLIERSGGVDFIYGESYL
                     RRDNAISLYRPELPLGPGLIAPLPGLDLAGSIRDGSPDAWGRRVIDSRLGVGDVDLTE
                     LSYLVASGSNRLGALDFQTSPTRYVPRDETATLDELHEAAIMVEEGRALPQPLAEALI
                     RGTSIGGARPKALLVDGDGTQWIAKFSSSSDTHFSVPNAEGATMILARHAGIDVCDVR
                     VTSSLGREVLLVRRFDRTASGGRIHAVSALTMSGESEMGARYVTYPQILNVLTRLSGS
                     RMPVGPALFRRIVFNVAVSNSDDHARNHAALWDGEHLRLSPAYDLCPGPRSGQTAEQA
                     MAFGRSGIRTSTFHAAISVAAEYGLSTSDARSVVDDVVSAITDHYAEAADQARLSEAD
                     RTAMLGRQFLNPGVWHGLPTTFALPASTAASPSPDQGSGEGD"
     gene            complement(4700..5434)
                     /locus_tag="PLK64_05840"
     CDS             complement(4700..5434)
                     /locus_tag="PLK64_05840"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglyceromutase"
                     /protein_id="HPV79296.1"
                     /translation="MAYTLILLRHGHSEWNAKNLFTGWVDVDLNDQGREEAVRGGRLL
                     KDKGVLPDIVHTSVLRRAITTANLALDAADRHWIPVRRDWRLNERHYGALQGLDKAAT
                     RAKYGDEQFMLWRRSFDTPPPPIDLDNEWAQTGDPRYAGIDIPRTECLKDVIARMMPY
                     WESAICPDLASGKTVLVTAHGNSLRALVKHLDGISDAAIAELNIPTGQPLVYDIGEDF
                     MPTGAGQYLDPEAAAAAAAAVANQGR"
     gene            5838..6074
                     /locus_tag="PLK64_05845"
     CDS             5838..6074
                     /locus_tag="PLK64_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HPV79297.1"
                     /translation="MSIHRSSFFVPAVVFTLSVFAVRLGDPVVWMWRDQPIVGILLLG
                     AAVVFWLLVLRQVRHARPPFEPPSTGQQQGRGRR"
     gene            6089..7222
                     /gene="moeB"
                     /locus_tag="PLK64_05850"
     CDS             6089..7222
                     /gene="moeB"
                     /locus_tag="PLK64_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HPV79298.1"
                     /translation="MSAQELTRYSRHIILPGVGVPGQQRLREARVAVIGAGGLGSPVI
                     LYLAAAGVGTIGVVDDDVVDVSNLQRQVLHSSSRTGMLKTESAKIAVHELNPHVEVIA
                     HSVRLSPENALSILAEYDIVVDGTDNYEARYLVSDACVLLGKPLVWGSVFQFEGQVSV
                     WWAGRGPCYRCAFPEPPAPGLVPSCAEGGVFGAMCASVGAAQVAEALKLVLGIGQPAL
                     GRLLVYDALGSRWRTLTLLADPQCPACGHAPTITDLDATRTVCGVASRDTLIAEVTPE
                     DLAAILADRPESVRLIDVRGPQERAIVSVPGDENIPLQQFEDGSALRLLSARDDLILY
                     CRSGIRSESAARLIPDGHVNSVKHLAGGILEWIRQVSPGLPTY"
     gene            complement(7216..8454)
                     /locus_tag="PLK64_05855"
     CDS             complement(7216..8454)
                     /locus_tag="PLK64_05855"
                     /inference="COORDINATES: protein motif:HMM:NF019429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfuA domain-containing protein"
                     /protein_id="HPV79299.1"
                     /translation="MAHGEARVIGLVDGVFGSVGSVWHKEILFAISRGCQVFGGASMG
                     ALRAAECRSFGIRGVGTVYAAYADGELEDDDEVAVSHLDAAVGYECLTVPMVTVRAAL
                     SAAVSVGLLPERDHERALKAAKNVFYADRTWDVLGREWGALDQDWGPDVTLFAQQPQH
                     DVKAVDAAAVLRAVQSARPEDGPRQADWQMEPTVFWRRLVQETDFPSVAPWFEDDGAQ
                     VSLARRIDVVRGALLDPGHAQLGTRARLFAHLVYQVCEALGIAPSAREVQERSERIRR
                     VLGLGDARTTRQWLLASKLTVAEWQRAIRFQLMAERLGEEYAAELRHQVPMLVAASGG
                     WAQVEHLAEPMQLGVEGESPSVVLTALATRPDMPSAATLEDLAARLGFTSLTEFLSTT
                     GVDPGDTQAATDVVERRAPQ"
     gene            complement(8562..9800)
                     /locus_tag="PLK64_05860"
     CDS             complement(8562..9800)
                     /locus_tag="PLK64_05860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HPV79300.1"
                     /translation="MTSVSTQDPVSEDLKRYRRGTHRACDPSETYDHLRALMPRCGIT
                     RIANITGLDRVGVPVYTAIRPNSRALSTSQGKGMDRDAARASALMEAFESWHGERVSA
                     DGGYYRREEAEDENGIPVADVGRISRRGRDVSWLSSTRINWVRGLDVMSGVAVLLPYD
                     CVSSDFTGDVQRVAFIRSTNGLASGNTLTEATLHGLYEVIERDAVALWSPSAGTADRV
                     VDPGTVHDEDNAALLDRLADAGLGVLIRDVRCDTRVPVFAVAIAPTNPTRMPPFAAFS
                     GYGAHLDPRVALSRALTEAIQSRLTVIHGGRDDLWPSAYARVLDHRAARNWARFVSTA
                     EPTVDFERIPDHSTETFSGDLEVLKESIASVAAEAIAVDLTRPGLGIPVVKVVVPGLS
                     GIPFDPRPRDGGRVGSAAPA"
     gene            complement(9797..10480)
                     /locus_tag="PLK64_05865"
     CDS             complement(9797..10480)
                     /locus_tag="PLK64_05865"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HPV79301.1"
                     /translation="MHVDGRSLSVLILKDCRMQWKAFLVLVMFEILTLLSFVAQFPEP
                     MRASAGAFLQGLTSIGTFVMAYRLLATEESSGTIRFLKSLPLTNDEIFWSKFLFMSGY
                     AVLNAAVVNLVYAGLRPLISWSWQLPTMPGVLGGVVVQLVFAVILLSIAILANSEKAI
                     WVPFPLVILLLNGYTLMTSPNGWDPGARVLASIQQNWALWSIAVVGVLLAVARGVVHA
                     AGVKRSLVK"
     gene            complement(10470..11192)
                     /locus_tag="PLK64_05870"
     CDS             complement(10470..11192)
                     /locus_tag="PLK64_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV79302.1"
                     /translation="MLSGRETLSLDGVGVRLGGFVLSGVTFAVGPGEVVGLVGHNGSG
                     KSTTFRIIADLIRPTSGSITLGDLDHRADERAFKRRVCFVGDNNQTYAGMTVGAVLAF
                     ARRLYPNWDHDWCAQLCDELRLPLDTKVAHLSAGMRTKLGLVLGFSPRPDVIVLDEPT
                     AGLDGASNEWIWQVVDRQSVEAGLAVIVSSHSRDDVAQHCSRVVLLEQGQIIDQIDIA
                     GDRALARERLEGAMGRGGGHAR"
BASE COUNT         1886 a         3812 c         3754 g         1840 t
ORIGIN      
        1 tgagtaagta ggccatgggg ctgactcagg cctcgaactt gtagcccaat ccgcgcacgg
       61 tgaggatgtg ccgcgggttg cccgggtcgg gctcgatctt ggcgcgcagc cgcttcacgt
      121 gcacgtcgag ggtcttggtg tccccgacgt agtcgctgcc ccagacccga tcgatgagct
      181 gcccgcgggt caagactcgc ccggcgtggc gcagcagcaa ctcgagcagc tcgaactcct
      241 tgagcggcat cggcgtcgcc ttgcccttga ccgtgaccac gtggcggtcg acgtccatcc
      301 gcaccgggcc ggcctcgacc gtggcgtcgg ccggctcgtc ggcctccgcc agccggcgca
      361 gcacggcgcg cactcgggcg agcagctcac gtgcggagta gggcttggtg acgtagtcgt
      421 cggcccccat ctcgagcccg acgaccttgt cgatctcgga gtctttggcg gtgagcatga
      481 tgatcgggac gctggacttg gctcgcagcg cacggcatac ctccacgccg gacaggcccg
      541 ggagcatgag gtccagcagc acgaggtcgg cgccgttggc gtcgaactcg gcgagggcgt
      601 cggggccggt cgcggcgacc gcgaccgcga agccctcctt ctccaacagg taggacaacg
      661 ggtcggagaa ggactcctcg tcttccacga tgaggattcg gctcatgcgg gtcgcacctc
      721 tctggtgacg tcgctgctct gtggacccgt cgaggccaac gccccgtcga ggtggtgatc
      781 gggtgaagct gggtcggcgg tgctggcggc ggggccaccc tcgcccccgc gcacatcgcc
      841 tggggcgagc acgccgatgg atccggtcgt ggactggtcg tcaggtccgt atgccggtgc
      901 aggcagccgg atcgtgaacg tcgatccccg gccgacttcg ctccacacgg tgacgtcgcc
      961 gccgtggttg gcgcacacgt gcttgacgat cgacaggccc aaccccgtgc ctccggtcgc
     1021 gcgcgaacga gccgcgtcga tccgatagaa ccgctcgaag atgcgcccca actcgctctc
     1081 agggatgcct tgtccctggt cggagacgat gatctcgcgg gcaccgtcag gggcgatcaa
     1141 ggcccgcacg gcgaccctag tgccggaggc ggagtaggcg accgcgttga cgacgaggtt
     1201 gcgcacagcg gtggtcagca ggcccgcgtc gccccaaacc cagcagttgg cgtcgcagcg
     1261 cacgacgagg tcgatgcggc gctcctcggc ggtgatccgg acgtcgtcga cggcctggcg
     1321 gacgacgccc cccaggtcga cggcatccgg gtcggacagc cggtcctcag actgcaggcg
     1381 ggagagctgg acgatgtcgc tgacgagttg gtccaggcga cgggtctcct tgcgcatccg
     1441 cttggcgaat cgggcgacgg cctccgggtc gtcacgcgcg tcgcgcacgg cctcggccag
     1501 gagcatgagg ccaccaacag gggtcttgag ctcgtggctg acgttggcga ggaagtcgcg
     1561 gcgcacctct tcgacgaggt aggcgtgcga gcggtcctcg acgagcaaga ggatgtgctc
     1621 caagcccagg tgggttgctc gcgcctgaac gacccgtcgg gcgtcggccc gggggccgcg
     1681 cggtaaccca agcatctctt cgacgacgcc gccctcgatg gcggcgcgtc gggccagggc
     1741 cagcaggggt gggtgcgaga cggcggtgcc gcggaccaga ccgtgcgaga tcgcggtggg
     1801 agaacaggcg acgaccgact gccgggaatc caggacgatg ccgctggacg tgagcgcggc
     1861 gagcaggtcg gtgacctccg cgggcaactg ttcgacagcc tcctcggggt cgggtgtggc
     1921 acctgcggcc aacgctcgac gacggtcgac acgggtccgc ccgaaccgcg cggagaccac
     1981 gacggcgccg aacacagcgc cgaggatcac cgcgagaagg acctccacgc ccctaggcta
     2041 gagggcaccc acgatgccga ggttcgggtg gggcgtgtcc ggcgcctttg cgggcccctg
     2101 catcgctcga tgttcacctg tgcgctgacc tatgttcacc gtggccgggc cgatgctgta
     2161 caccgtggcc gcccagccac gaccccccgc ttccccaccc ggatccgcga aggagcagac
     2221 gtgcgcgacg ccttccacga gcagttggac agcatcacgg cccaactggt cgacatgacc
     2281 cgcctggccg gttcggcgat ggcgcgagcc accaccgcgc tgttggacgc tgaccttcat
     2341 ctggctgaag acgtcatcac cggcgaccgc acgttggaca acgcccggga gcacctggac
     2401 agcctggcca tcgacgtcct ggcccgtcag caaccggtcg ccaccgacct gcgcaccgtc
     2461 gtcacggcgc tgcggatgag tggggacctg gagcggatgg gagacctggc ccgacacgtg
     2521 gccaaggtcg cccgactgcg cttcccgaac tcggccgtcc cgccgtcatt gcgcgcccac
     2581 atcctgcaga tgggccaggt cgccgagttg gtggtcacca agtcgggctc gatcatcgct
     2641 gctcgcgacg tgaccgaagc gcgcaatctt gagcgcgacg acgacgtcat ggacgagttg
     2701 caccgccggt tgttcaccgt gctcgcgacc accgagttgc cgaccgagac gatcgtcgac
     2761 ctcaccctgt tgggtcgcta ctacgagcgg ttcgccgacc atgccgtgtc ggtggcgcgt
     2821 cgcgtcgtct acctcgtcac gggcgaatac gtcccagaga cgtcagggga tcacgacgtc
     2881 tccgaggagg gcgcgcggat cgcgctcctt cgccgcagca gcccgcgggg ctgacgagtg
     2941 ctcacgccct ccttgccgta tcgggcttga ccgctgactc ctgccgggca ggagttatat
     3001 actgcacctt acgagtgtta aagggtgctt tattggagga cctatggcca agcgcgccat
     3061 cgttgcgcaa cccgaggtgc tcgacgctgc tcggacgctg ggcgtgcaga tccgcatcgc
     3121 gcggcacgag cgcgggtgga ctgccgccga cctcgccagc aagatcggcg tctcggccaa
     3181 aaccgttctc gcgatcgaag ccggggctgt gtccacttcg atcggcacgg tcttcaacgc
     3241 ggccgtcatg gtcggcgtgc cgctgttcgg ggtcgaggac cggttcgaga tggctcggat
     3301 gcgccgacgc ggagaggaac gtctggccct gatcggccag cgggtccggg tgccgaagct
     3361 ggacgagacc gaccttgcca acttctaagc ccccacagtc gctgttcgtc tggacctggc
     3421 tgcccggcga caccactccc gtcgtcgccg ggcgcctgat cgagcgctcc ggcggggtcg
     3481 acttcatcta tggcgagtcc tacttgcgcc gcgacaacgc gatcagcctc taccgcccgg
     3541 agttgccgct cggccccggc ctgatcgcac cgctccctgg gctcgacctg gcggggagca
     3601 tccgtgacgg atcccccgac gcctggggcc ggcgcgtcat cgactcgcgc ctgggtgttg
     3661 gggatgtcga cctgaccgag ttgagctacc tcgtcgcatc cggctcgaac aggttagggg
     3721 cgctggactt ccagaccagc ccgacccggt atgtcccgcg agacgagacc gcgaccttgg
     3781 acgagctgca cgaggcggcg atcatggtgg aggaagggcg cgctctcccc cagccgctgg
     3841 ctgaggcact gatccgcggc acgtcgatcg gcggtgcccg gccgaaggcg ttgctggtcg
     3901 acggcgacgg cacccagtgg atcgccaagt tctcatcgtc gtcagacacc cacttcagcg
     3961 tcccaaacgc cgaaggggcc acgatgatcc tggcgcggca cgccgggatc gacgtctgtg
     4021 atgtgcgggt gacctcgtcg ttagggcgcg aggtccttct ggtccggcgg ttcgaccgca
     4081 ccgcttccgg cggccggatt cacgcggtgt ccgctctcac catgtcgggt gagagcgaga
     4141 tgggcgcccg ctatgttacc tacccgcaga tcctcaacgt cctcacgcgg ctttccggca
     4201 gccgtatgcc ggtcgggccc gccctcttcc gacggatcgt cttcaacgtc gccgtcagca
     4261 actccgacga ccacgcgcgc aaccacgcgg cgctgtggga cggcgaacac cttcgcctct
     4321 caccggcgta tgacctctgc ccagggccgc gctccggaca gaccgctgag caggcgatgg
     4381 cgttcggacg cagcgggatc cgcacgtcca ccttccacgc cgccatcagc gtggccgccg
     4441 aatatggcct gagcaccagc gacgcccgat ccgtggtgga cgacgtggtg tcagcgatca
     4501 ccgaccacta cgccgaggcc gctgaccagg cccggctgag cgaagccgac cggacggcca
     4561 tgcttgggag gcagttcctc aaccctggcg tctggcacgg attgccgacc accttcgcgt
     4621 tgccggcctc aacagccgcg tcgccctcgc cagaccaagg gtccggcgag ggcgactgac
     4681 tggctcgatc cgcgtcggct cagcggccct gattggcgac ggccgcagcg gcggcggcag
     4741 cagcctcggg gtcgaggtac tgccccgctc cggtcggcat gaagtcctcg ccgatgtcgt
     4801 agaccaacgg ctggccggtc gggatgttga gctcggcgat cgccgcgtcg ctgatcccgt
     4861 cgaggtgctt gaccagggcc cgcaggctgt tgccgtgggc ggtgacgagc accgtcttgc
     4921 cggaagccag gtcggggcag atcgcgctct cccagtaggg catcatccgg gcgatgacgt
     4981 ccttgaggca ctcggtgcgc gggatgtcga tgccggcata ccgggggtcg ccggtctggg
     5041 cccactcgtt gtccaggtcg atgggcggcg gcggggtgtc gaaggagcgg cgccacaaca
     5101 tgaactgctc gtcgccgtac ttcgcgcggg tcgcggcctt gtccaggccc tgcaacgcgc
     5161 cgtagtgccg ctcgttgagc cgccagtccc ggcgcaccgg gatccagtgc cggtcggcgg
     5221 cgtcgagcgc caggttggca gtggtgatcg cccggcggag cacggacgtg tggacgatgt
     5281 cgggcaggac gcccttgtcc ttgagcagcc gcccgccgcg gacggcttcc tcgcggccct
     5341 ggtcgttgag gtcgacgtcg acccacccgg tgaagaggtt cttggcgttc cactcggagt
     5401 ggccgtgacg cagcaggatg agggtgtatg ccatgcggcc cagcctattg cgaccgatcg
     5461 cctcagggtc tagcggtcca gcgtccgcta cgtcacgctc ctccgcgcgc tgacctgagg
     5521 cgggcgacca tggccggcct gggactatct cagattcggt gtttctggtc cgtggcggac
     5581 cagaaacacg aatttgagat agtccccatg gcgaacgcat ggggagggtc ggccccgccg
     5641 ctttacttcg gctgtgggca agagttccga ccggggcccg gtgggagggg aagcggcact
     5701 tctccccagg cgtattcaga gcgccgggga tacacttggg gacagtgaga tttcgttcgt
     5761 cgatgcggac ggaaaaggta cccctggaag cgctcgggcg cccctctcaa agggcacccg
     5821 ctggaaagga cgctctcgtg tcgatccatc gctcgtcgtt cttcgttcca gccgttgttt
     5881 tcaccctgtc cgtgtttgcc gtccgcctcg gggaccccgt ggtttggatg tggcgcgacc
     5941 agccgatcgt cgggatcctt ctcctcggcg ctgcggtcgt cttctggctg ctggtcctgc
     6001 ggcaggtccg gcatgctcgg cctccgtttg agcctccgtc gaccgggcag cagcaaggcc
     6061 ggggccggag atgactgaca caaggtcctt gagcgcccag gagttgactc gctactccag
     6121 gcacatcatc ctcccgggag tcggcgtgcc gggccagcag agactcagag aggcgcgggt
     6181 cgccgtcatc ggcgcaggcg gactcggatc accggtgatc ctctacctcg cagccgcggg
     6241 tgtcggcact atcggggtgg ttgacgacga tgtggtcgac gtgtccaacc tgcagcggca
     6301 ggttctgcat agttcgtccc gcactgggat gctcaagact gaatccgcca aaatcgctgt
     6361 gcacgagctg aatccacatg ttgaagtgat cgcccactcg gtccggctgt cgcccgagaa
     6421 cgcgttgtcg atcctcgctg agtacgacat tgtggttgac ggcacggaca actacgaagc
     6481 gcgctatctc gtctcagacg cttgtgtgct cctcggcaag ccactcgttt gggggtcggt
     6541 cttccagttt gagggccagg tatccgtgtg gtgggcaggg cgcggcccat gctaccgatg
     6601 cgcctttcct gagcccccag ccccgggtct tgtcccgtcg tgcgcggagg gcggggtgtt
     6661 cggagccatg tgcgccagcg tgggagcagc ccaggtcgcc gaagcactca agctcgtact
     6721 cgggatcggc cagccggcgc tcgggcgcct gcttgtctac gacgcgctcg gatcacgctg
     6781 gcgaacgctg acgctcctgg ccgatccgca gtgcccagcc tgtggacacg cccccaccat
     6841 caccgacctc gacgcaacga ggactgtctg cggggtggcg tcgagggaca ctctcatcgc
     6901 tgaggtcacg cctgaggact tggccgcaat ccttgctgat cgaccggaat ctgtccggct
     6961 cattgacgtg agagggcctc aagaacgtgc cattgtctcc gtacccggag acgagaatat
     7021 cccactacag cagttcgagg acggatcggc acttcgtctg ctatcagcgc gcgacgacct
     7081 tattctctac tgtaggtctg gaatacgctc cgaaagcgcg gcccgcctta tcccagatgg
     7141 ccacgtcaac agcgtcaagc atctcgccgg cggcattctg gaatggatac ggcaggtgtc
     7201 tccgggcctg cccacctatt gaggcgccct cctctcgacg acgtccgtgg cagcctgagt
     7261 gtcccccggg tcaacgccag tggtcgacag gaactcggtc aacgaggtga acccgaggcg
     7321 agcggccaga tcctcaagag tggccgctga cggcatgtct ggtcgagtcg ccagcgccgt
     7381 cagcaccact gatggcgatt cgccctcgac gcccaactgc atcggctcgg ccaggtgctc
     7441 gacctgggcc cacccgcccg aggccgcgac cagcatcgga acctggtgcc gcagttccgc
     7501 ggcatactcc tcgcccagcc gctctgccat caactggaac cggatggccc gctgccattc
     7561 cgctacggtc aacttggagg cgagaagcca ctgccgagtg gttcgggcat cacccaggcc
     7621 gagtactcgc cggatccttt ctgagcgctc ctgcacctca cgcgcggatg gggcgatgcc
     7681 tagagcctcg cagacctgat agaccaagtg ggcgaacaac ctggcccgcg tgccgagctg
     7741 cgcatggccg ggatcaagaa gagcgccccg gaccacgtcg atacggcggg cgagtgaaac
     7801 ctgcgcgccg tcatcctcga accacggagc cacggacggg aagtcggtct cctgcaccag
     7861 ccgacgccag aagacagtcg gttccatctg ccagtccgcc tggcggggac cgtcctccgg
     7921 gcgagccgac tggacggccc tcagcaccgc ggccgcgtcc acagccttga cgtcgtgttg
     7981 cggctgctga gcaaacaggg tgacgtccgg gccccagtcc tgatcaagag caccccactc
     8041 ccgcccaagc acatcccagg tccgatccgc gtagaagacg ttcttcgcgg ccttcagtgc
     8101 ccgctcatgg tcgcgctcgg gcaggaggcc gacgctcacc gcggcactca gcgcggcacg
     8161 cacggtcacc ataggcaccg tgaggcattc gtagccgact gccgcatcca gatgcgagac
     8221 cgcgacctcg tcatcgtctt ccaactcccc gtcggcatat gcggcataca ccgtgccgac
     8281 tccgcggatg ccgaaacttc gacactccgc cgcacgcaac gctcccatgc tcgcgccgcc
     8341 gaagacctga cacccacggg agatggcgaa gaggatctcc ttgtgccaga ccgatcccac
     8401 tgatccgaag accccgtcga ccaaaccgat gacgcgggcc tctccatgcg ccaccgcacg
     8461 atagacatcc cctgcgcgtg cgggcgggag caaggtgact tccggcgcga gcgcttgcgc
     8521 ggcgttcggc gggatgctcg ggccgacaaa ggcaatcctc ctcacgcggg tgccgccgac
     8581 ccgactcggc ctccgtcgcg gggacggggg tcaaagggga tgcccgagag gccaggaacg
     8641 acgaccttca ccacagggat gccgaggccg ggacgggtca ggtcaacggc gattgcctcg
     8701 gcggccaccg aggcaatcga ctccttgagg acttcgagat ctccggagaa ggtctccgtg
     8761 gagtggtcgg ggatccgctc gaagtccacc gtcggctcgg ccgtggatac gaaccgagcc
     8821 cagttccgag cggcccggtg gtcaaggact ctggcatacg cggaaggcca aagatcgtct
     8881 cgacccccgt gaatcacggt gagccgactc tggatggcct cggtgagtgc ccgagagagc
     8941 gccactcgtg ggtccagatg ggcgccgtac ccggagaagg cggcaaaggg cggcatccga
     9001 gtcggattgg tgggcgcgat cgcgactgcg aacaccggca ctctcgtgtc gcacctcacg
     9061 tcgcggatca gcacgccgag cccggcgtcc gcgagtcgat cgagaagtgc ggcgttgtct
     9121 tcgtcgtgaa ccgttcccgg gtcgacaacc cgatcggcag tgccggcgct cgggctccaa
     9181 agggcgactg cgtcgcgctc aatcacctcg tacaacccat ggagggttgc ctccgtcagg
     9241 gtgttaccgc tggctagacc gttcgtcgaa cggatgaacg caacccgttg cacgtctccg
     9301 gtgaagtcgc tggacacaca gtcgtacggg agcagaaccg caacgcccga catgacgtcc
     9361 aggcccctca cccagttgat ccgcgtcgat gacagccacg agacgtctcg acctcggcgg
     9421 ctgatccggc ccacgtcggc caccgggata ccgttctcgt cctccgcttc ttcccgtcgg
     9481 tagtagcccc cgtcggcact gacccgctcg ccatgccagg actcgaaggc ttccatcagc
     9541 gccgacgctc gcgctgcgtc ccgatccatc ccctttccct gggacgtcga gagcgcacgg
     9601 gagttcggtc ggatcgcggt atacaccggg acgccaaccc ggtcgagtcc ggtgatgttc
     9661 gcaatgcggg tgataccgca cctgggcatg agcgcccgca agtgatcgta ggtctcggac
     9721 gggtcgcagg cgcggtgcgt cccccgccga taccgcttga ggtcctcgct caccggatcc
     9781 tgggtagaga cggacgtcat ttcaccagac tccgcttgac cccggccgcg tgcaccaccc
     9841 cgcgggccac cgcgagaagc acaccaacga ccgcgatcga ccacagggcc cagttctgct
     9901 ggatcgaagc caggacgcga gcaccggggt cccagccgtt cggcgacgtc atcagggtgt
     9961 agccgttgag caggaggatg acgaggggga agggcaccca gatcgccttc tcggagttgg
    10021 ccagtatcgc aatggacaac aggatcaccg cgaagaccag ctgtacgacc accccgccaa
    10081 ggaccccggg catcgtcgga agttgccagc tccacgagat gagggggcgc agcccggcat
    10141 acacaaggtt gacaaccgca gcgttgagaa cggcgtagcc ggacatgaag aggaacttcg
    10201 accagaagat ctcgtcgttg gtcagcggga gcgacttgag gaaacggatc gtgccgctgg
    10261 actcctcggt cgccaacagc cggtaggcca tcacgaacgt gccgatggag gtcagacctt
    10321 gcaggaaggc gcccgctgag gcgcgcatgg gttctgggaa ctgggccacg aagctcagca
    10381 gggtgaggat ctcgaacatc accagaacga gaaaggcctt ccactgcatt cgacagtctt
    10441 tgaggatcag gacgctcagg ctcctgccgt caacgtgcat gcccacctcc tcggcccatc
    10501 gcaccttcca accgctcgcg ggcgagggca cggtcgccgg cgatgtcgat ctgatcgatg
    10561 atctgcccct gctcgagaag caccacccgg gaacagtgct gagcaacgtc gtcgcgggag
    10621 tgtgaggaga cgatcacggc cagcccggcc tcgaccgact ggcgatccac cacctgccag
    10681 atccattcgt tggaggcccc gtccaggcca gcagtgggct cgtcgagcac aatgacgtcc
    10741 ggccgtgggc taaagcccag gaccaggccg agcttcgtcc gcatgcctgc cgacaaatgc
    10801 gccaccttgg tgtccaacgg aaggcgcaac tcgtcgcaca gctgcgcgca ccagtcgtgg
    10861 tcccagttgg ggtagagccg tcgggcgaag gcgaggaccg caccgacggt cataccggca
    10921 tacgtttggt tgttgtcgcc gacgaagcag acccgtcgct tgaacgccct ttcatcggca
    10981 cgatggtcga ggtcgcccag ggtgatcgag cccgatgtgg gccggatcaa gtcggcaatg
    11041 atacggaagg tcgtgctctt tcccgagccg ttgtgtccca caagtccgac gacctcgccc
    11101 ggtccgacgg cgaaggtcac cccggacagg acgaacccgc caaggcgaac cccgactccg
    11161 tcaagggaca gagtttcacg accgctgagc atgtgcaccg ccttcgagga atacttgccg
    11221 gcgcagggcc ttggtgtagc gccatgcgtt gagcagggcc aacagaccca ggacgactcc
    11281 gagaacaagg gc
//
LOCUS       DAOMZY010000074        11287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5391_length_11287_cov_5.555021, whole genome shotgun sequence.
ACCESSION   DAOMZY010000074 DAOMZY010000000
VERSION     DAOMZY010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 11287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11287
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5391_length_11287_cov_5.555021"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PLK64_05875"
     CDS             <1..580
                     /locus_tag="PLK64_05875"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HPV79303.1"
                     /translation="EAIRTLGSLDIVEVRHGHGTFVGRMSLDPLVNGMVFRLSMRGEH
                     AVTALRNVVQTRIALDLAMTDDLVKAYNGTYDSSMHALVDDMRTHTEQGHAFLDEDRD
                     FHARLAKGVDNQIIGELAGAFWEIHTRALPLLGISTPQDIMDTVEAHEAILLAVESGD
                     VEAYRSAVQAHYLPLQRAIERAAHMIDDEPTP"
     gene            582..2288
                     /locus_tag="PLK64_05880"
     CDS             582..2288
                     /locus_tag="PLK64_05880"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPV79304.1"
                     /translation="MSRADVVIAESGVGYRQYRIPALALTPAGTCLAVFDARCDLDDL
                     PAPVDLVARRSTDGGRSFGPQTVVRAGTGVIGHGDASLVVDHESGSVFAFHATSAGVG
                     FFESSESIKDDDLGVLHCDVSVSHDDGVTWEHRRLSRDLRVGLNARLPEVGLGEAARV
                     SGLFAASGSGTQIRAGRFHGRLLQGFVALIGTQVYAAVAHSDDHGRTWVMGRPVGPGA
                     NENAVVWLGGDRVGLQSRAPGQRLMAWSDDGGDTFGPLEPAGDLLDPGNNGSLVRLDP
                     PGSVSTGDTGDTGDTGDGDDAGDPTGIRLACTHTSDPDLRRNLVVSTSTDDGATFTPA
                     VTLTAGAAGYSVAQPLPGGALGVLWEDEGYRRLIFTRLEPGWELPRAAHPAQGPAPAL
                     VLDHVAAGHPATTSSPTPAWVAHPPIVASEWGPTIYKIVASRPGGPMLRSRAAYRELL
                     GDPAPGLHVNDILTFSARFPLPQVTDVRLDGVPVSPSHIVPVGSGTVLRGLRRVVRAQ
                     DVEAGVALVRVSATDGDRTAVLSARVPVELATASADPANPTRPAATAPAPAPLPVPLR
                     ST"
     gene            2285..2983
                     /locus_tag="PLK64_05885"
     CDS             2285..2983
                     /locus_tag="PLK64_05885"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012804459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HPV79305.1"
                     /translation="MNPLIAALRGTLVVSCQAYPGEPMRHPETMAQVAESAVRGGAAA
                     IRAQGLADISHIAGRVDVPVIGLWKHGRGPVHITPTLRHARAVLDAGADIVAIDATAR
                     PRPDGLTVAQTVAGLRHSHPGAIIMGDCGSVDDGLTAADAGVDVVATTLSGYTGDRPR
                     TDGPDLELLAELVAALPHHAIVAEGRIHTPAQASAALAAGAHCVVVGTAITHPQSITG
                     WFAAALTSPVPQLP"
     gene            3046..3828
                     /gene="nagB"
                     /locus_tag="PLK64_05890"
     CDS             3046..3828
                     /gene="nagB"
                     /locus_tag="PLK64_05890"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HPV79306.1"
                     /translation="MEVVITTPPQAASLVADAVTALFRGRPDAVLGVATGSTPVLVYN
                     ELGRRVERGELSLARARAFMLDEYVGLPEGHPERYRTVIDRDFASKVDIDPANVHGPD
                     GLAKDLPGACAAYEAAIQAAGGVDLQLLGIGSDGHVAFNEPGSSLASRTRIKSLTRQT
                     REDNARFFDGDTEAVPRHCLTQGVATILAARHLVLMAFGKGKAEAVHHLAEGPVSAMW
                     PATALQLHPHVTVFVDDAAASRLQLGAYYRETFQAKPSWQGI"
     gene            complement(3889..4005)
                     /locus_tag="PLK64_05895"
     CDS             complement(3889..4005)
                     /locus_tag="PLK64_05895"
                     /inference="COORDINATES: protein motif:HMM:NF024682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79307.1"
                     /translation="MAADFAGAADDAARLRVVLDQVASLTDARAVTLARTHA"
     assembly_gap    4032..4131
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4132..5270)
                     /locus_tag="PLK64_05900"
     CDS             complement(<4132..5270)
                     /locus_tag="PLK64_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HPV79308.1"
                     /translation="MTYAARDRERWVAEDPAHKQSDRDDFARDRARVLHSAALRRLSA
                     KTQVVAPGSDDFIRTRLTHSLEVAQIGREFGSALGCDADVVDTACLAHDLGHPPFGHH
                     GESVLDELASDAGGFEGNAQTLRVLTRLEPKRVHDDGRPAGLNLTRASLDAVVKYPWA
                     RGQGPYETAKYGVYAADLPVFQWFREGADVGVRCLEAQVMDWSDDVAYSVHDVEDAIA
                     SGRVDPRVLYSPTEVRVVAELARTAYAPDLPVEAFVAAAQRLVATGTVPHSFAGTRAD
                     LAALKDMTSRLIGRFLSAVEMATRARYGPGPLTRFAADLVIPDDVRAECSLLKAVAAH
                     YVMFTDERRSVMGRQEEVVRTLVEGYRRHPERLDADLAADFAGAA"
     gene            complement(5267..5794)
                     /locus_tag="PLK64_05905"
     CDS             complement(5267..5794)
                     /locus_tag="PLK64_05905"
                     /inference="COORDINATES: protein motif:HMM:NF024124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3806 domain-containing protein"
                     /protein_id="HPV79309.1"
                     /translation="MGLFSRRKDDPSEDVAPLDGVGEGELIDAEADDDWSRPLGPREL
                     PLRADHRALLDGCLAELEAAGIDLDDIAAIGAGYDAECAAWGARGGLRSAKAGDQDPV
                     IARWGVAIGEHLARTTDLRWVTVEDPFGTDLGLFAESDYFALVPTNLVSGRFLNGEIG
                     WVPGVVGHLVELRNR"
     gene            complement(5804..6484)
                     /locus_tag="PLK64_05910"
     CDS             complement(5804..6484)
                     /locus_tag="PLK64_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HPV79310.1"
                     /translation="MTTQTEPPAGQPERPATFFESAAEFRDWLAANHETAPELWMGLR
                     KKHVEPRGLTWAEAVPEALCYGWIDSVVQSLGADAVRQRWTPRRNGSTWSKINVALVA
                     QLEAEGRMQPAGRAAYERGRADRTGIYAYEQADSGAFPPEYAARLAASPAAPAFWERA
                     PASYRKQVIHWVLSAKQQATRDRRMDQLIESCAAYLPIPPLRYGDEAGWMRRARVELG
                     EPPAPPRP"
     gene            complement(6486..7661)
                     /gene="dusB"
                     /locus_tag="PLK64_05915"
     CDS             complement(6486..7661)
                     /gene="dusB"
                     /locus_tag="PLK64_05915"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HPV79311.1"
                     /translation="MTAVAQSVLPVLPALPIGRHTVSTPVVLAPMAGITNRAFRRLCR
                     EHAPGALYVSEMITSRALVERNAETMRLIEHDADESPRSVQLYSVDPVTARAAARILV
                     QEDRADHIDLNFGCPVPKVTRKGGGSALPWKRDLFRAIVAAVVAEGARRDIPVTVKMR
                     IGIDDDHLTYLDAGVAAEQEGAAAVALHARTAAQHYSGYAVWPAITALKQAVTTIPVL
                     GNGDIWSADDAVRMVAETGCDGVVVGRGCLGRPWLFADLAAAFEGSTLRVQPTLGEVA
                     DTMRRHAAYLVEFYDDENRGCRDIRKHIAWYLKGFPAGGTMRQHLALVDSLAALDRLL
                     DGLDADAPWPGADAEGQRGRAGTPKRVALPDGWLDSPDLSPEQQALVAQAELDVSGG"
     gene            complement(7793..10060)
                     /locus_tag="PLK64_05920"
     CDS             complement(7793..10060)
                     /locus_tag="PLK64_05920"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPV79312.1"
                     /translation="MTTHVSSSAIPDSAAADPTGMPPSPHGQTKPRVEPPAGVPRSSV
                     AAWCVTHRWWVLALATLGLVGSVMLLSTGIVTTPQEQSLVGESARAQTLISQADFGDR
                     PTERIIATVRTGSLAPEQAAALGAELAGAYQGVEGVARVGDPIPGADGRSVVIPLELA
                     AARSATDTTLPQPEEVVAPSLAVTTRLGAARPDLQVGQIGPGSITREFDATLGRDFQR
                     AEFVSLPVTLVILLIAFGAVVAAGVPLVLGIGSVAVALGLTAAVSNHLVTVDQNTQSM
                     VLLIGLAVGVDYALFVMRRAREERAAGASVEDSIVLAGATAGRAVIVSGLTVMVAMSG
                     MLVAGGLFTSLAIGTSLVVGVAVLASATVLPALLAVLGDRVDALRLPGTRRRQANAGS
                     VESRWGRLAGRVVARPVAWAATAAVALIALSVPALGMRTALTDVEGLPKDLAVVAAYR
                     QLSAAAPTKTVALLLTVKAPASAAPAVAEALAAAAPRAAALAHVSGVQPQASVSKDGT
                     VTLLPIGVALDASDERLPGVVDAVRTQVAPQVTAALRGRPGVEVVVGGEAAATDLAQW
                     MDSRLPWVVGFVLILTLVVMLISFGSPWLAVATVGLNLLSVGAAYGVMTLVFQGTWAE
                     RLLDFTSIGSIAAWLPLLMFVVLFGLSMDYHVFVTSRVREARDAGLDARAAVRLGVAR
                     SAGVVTSAAAVMVGVFAVFGTLSVLELKQLGVGLAVAVLLDATVVRGVLLPAVLALLG
                     DRAHQGPAWLPRLHH"
     gene            complement(10234..10482)
                     /locus_tag="PLK64_05925"
     CDS             complement(10234..10482)
                     /locus_tag="PLK64_05925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HPV79313.1"
                     /translation="MRTTIDAAGRVVLPADVRDALGLAAGSEVDLVLIDGRIEITIAA
                     VEGTVSVEDGWPQLTPTRADQPTLDDQTVQDTIRINRR"
     gene            complement(10498..>11287)
                     /gene="miaE"
                     /locus_tag="PLK64_05930"
     CDS             complement(10498..>11287)
                     /gene="miaE"
                     /locus_tag="PLK64_05930"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 -
                     tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl
                     adenosine)-hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /protein_id="HPV79314.1"
                     /translation="LRPDLGNPYAQALHKLASKGLLDRLLIAALIEARSCERLWLLEQ
                     AGPAQAELGSAGWLAFLLELERCEAGHAVAYAVLFGLVPLQLTAHATGDADGFLCAHD
                     VHQTRDSMLRMATVALAGGLAEELIFGAEHASAGRASDRERATSLVLDSVRRLGFGSW
                     ALDYGLEFAYATDASVTEPTAEALLASLADRAAELLRAHRGALIALAQALAAAGSLSG
                     DAVSDVLGREGIVAEVRSEGFQWTPPYAAALAAAAARHGLAVAR"
BASE COUNT         1674 a         4135 c         3848 g         1530 t
ORIGIN      
        1 ggaggcgatc cgcacgctgg ggtccctgga catcgtcgag gtgcgtcacg gtcatggcac
       61 cttcgtcggg cggatgtcgc tggacccgct cgtcaacggg atggtgttcc ggctgtcgat
      121 gcgtggcgag cacgcggtga ctgccctgcg taacgtcgtt caaacccgga tcgccctcga
      181 cctggcgatg accgacgatc tcgtcaaggc ctacaacggc acctacgact cgtcgatgca
      241 cgccctcgtg gacgacatgc gcactcacac cgagcagggc cacgccttcc tcgacgaaga
      301 ccgcgacttc catgcccgcc tcgccaaggg ggtggacaac cagatcatcg gcgagctcgc
      361 cggggccttc tgggagatcc acacccgcgc cctccccctg ctggggatca gcacgccgca
      421 ggacatcatg gacaccgtcg aggcccacga agccatcctt ctcgccgtcg agtccggcga
      481 tgtcgaggcc taccgcagcg ccgtccaggc ccactacctg ccactgcaac gagcgatcga
      541 gcgggccgcg cacatgatcg acgacgaacc gaccccctga gatgtcgcgc gccgatgtcg
      601 tcatcgcgga gtccggagtg ggctaccgcc aataccgcat ccctgcgctc gccctcactc
      661 ccgccgggac ctgcctcgcc gtcttcgatg cccggtgcga cctggacgac ctgcccgcgc
      721 ccgtcgacct cgtcgcgcgc cgctccaccg acggcggtcg ctcgtttggc ccacagaccg
      781 tcgtgcgggc ggggaccggc gtcatcggcc acggcgacgc gtccctcgtt gtcgaccacg
      841 agagcgggtc cgtcttcgcc ttccacgcca cctccgcagg ggtcggcttc ttcgagtcgt
      901 ctgagtcgat caaggacgac gacctcgggg tgctgcactg cgacgtgtcg gtctcccacg
      961 atgacggggt cacctgggag catcggcggc tcagccgcga ccttcgcgtc ggcctcaacg
     1021 cccgcctccc cgaggtcggc ctgggagagg cggcccgggt gtcgggcctg ttcgccgctt
     1081 ccgggtccgg gacccagatc cgcgccggac gctttcacgg tcggctgttg caaggcttcg
     1141 tcgcactcat cggcacgcag gtgtatgccg ccgtcgccca cagcgacgac cacggccgca
     1201 cgtgggtcat gggccgtccc gtggggccag gcgcgaacga gaacgccgtc gtctggctgg
     1261 gtggcgaccg cgtcggcctg cagagccggg cacctggcca acgcctcatg gcctggtccg
     1321 acgacggcgg tgacacgttc gggccgctgg agccggccgg ggatctcctg gacccgggga
     1381 acaacggcag cctcgtgcgc ctcgacccgc cgggcagcgt gtccacgggc gacacgggcg
     1441 acacgggcga cacgggcgac ggcgacgacg ctggcgatcc caccggcatc cggttggcgt
     1501 gcacccacac cagcgacccc gaccttcggc gcaacctcgt ggtgtccacc tccaccgatg
     1561 acggggccac cttcaccccg gcggtcaccc tcaccgcggg ggctgcggga tactccgtcg
     1621 cccaaccact gcccgggggt gcccttgggg tcctgtggga ggacgagggc taccgtcgcc
     1681 tgatcttcac ccggcttgag cccggctggg aactcccccg cgccgcacac ccagcgcagg
     1741 gcccggcccc cgcgctcgtc cttgaccacg tcgctgcggg ccacccggcc accacgtcga
     1801 gcccgacccc cgcttgggtc gcccatccac cgatcgtggc gagcgagtgg ggtcccacca
     1861 tctacaagat cgtcgcctcc cggcccggcg gtccgatgct gcgcagccgc gcggcatacc
     1921 gggagctcct cggtgacccg gcaccagggc tgcacgtgaa cgacatcctc accttctcgg
     1981 cccgcttccc tttgccgcag gtcaccgacg tgcgcctgga cggcgtcccc gtctcaccca
     2041 gccacatcgt gcccgtcggc tccggcacgg tgctgcgcgg gctgcgccgc gtcgtccggg
     2101 cgcaggacgt cgaggccggc gtggcgctcg tgcgggtgtc ggccaccgac ggtgaccgca
     2161 ctgcggtcct gtcggcgcgc gtcccggtcg agctcgcgac cgcctcggcc gaccccgcca
     2221 acccgactcg tccggccgct acggcacccg ctcccgcacc cctcccggtt cctctgaggt
     2281 ccacgtgaat ccactgatcg ccgccctgcg cggcaccctt gtcgtctcct gtcaggccta
     2341 tcccggcgag ccgatgcgcc accccgagac gatggcccaa gtggccgagt cggccgtccg
     2401 tggcggcgcc gcggccatcc gggcccaagg gctggcggac atctcccaca tcgccggtcg
     2461 ggtcgacgtg cccgtcatcg gcctgtggaa gcacggccgg gggccggtcc acatcacgcc
     2521 gaccctgcgg cacgcgcggg ccgtcctcga cgccggcgcc gacatcgtgg cgatcgacgc
     2581 aaccgcccgc ccccgccccg acgggctcac ggtggcccag acggtggccg ggctgcgaca
     2641 cagccatccc ggcgcgatca tcatgggtga ctgcgggtcg gtcgacgacg ggctgaccgc
     2701 cgccgacgcc ggggtcgacg tggtcgccac gacgctgagc ggctacaccg gtgatcggcc
     2761 gcgcaccgac ggccccgacc ttgagctcct ggccgagctc gtggccgccc tcccccatca
     2821 cgcgatcgtc gccgagggac ggatccacac tccggcgcag gcgagcgctg cgctggctgc
     2881 cggcgcgcac tgcgtcgtcg tcggaacggc catcacccat ccccagtcga tcactggctg
     2941 gttcgccgcc gcgttgacct cacccgtgcc gcaactcccc tgatctcgtc catcgttgat
     3001 ctcaaccatc gccaaaccgc aacccagata ggaggcagac ctcagatgga ggttgtcatc
     3061 accaccccgc cccaagcggc gtccctcgtt gccgacgctg tcaccgcgct cttccggggg
     3121 cggcccgacg ccgtgctcgg cgtcgcgacc ggctccacgc cggtgctggt ctacaacgag
     3181 ctcggccgac gggtcgagcg cggcgagctc agcctcgccc gcgcccgggc gttcatgctc
     3241 gatgaatacg tcgggttgcc tgaaggacat ccggagcgct accgcacggt catcgaccgc
     3301 gacttcgcca gcaaggtcga catcgacccg gccaacgtcc acggccccga tggtctggcg
     3361 aaggacctgc cgggggcatg cgcggcatac gaagcggcga tccaagccgc cggcggagtg
     3421 gacctgcaac tgctcgggat cggctccgac ggtcacgtcg ccttcaacga gcccggctcc
     3481 tcgctggcct cacggacccg catcaagtcg ctgacccgcc agacgcggga ggacaatgcc
     3541 cgcttcttcg atggtgacac cgaggcggtg ccgcggcact gcctcaccca gggtgtggcg
     3601 acgatcctcg cggcccggca cctggtgctc atggccttcg gcaagggcaa ggccgaggcc
     3661 gtccaccacc tggccgaggg gccggtgtcg gccatgtggc cggcgacggc gctgcaactg
     3721 cacccgcacg tcaccgtctt cgtcgacgac gctgccgcct ctcgactgca actgggcgcc
     3781 tactaccggg agaccttcca ggccaagccg agttggcagg ggatctgacg ggctgaggat
     3841 tgcgcctctg cctgcgcgag cgttcgtgcg tgcgtcggcg cggggcgcct aggcgtgcgt
     3901 gcgcgccagg gtgacggcgc gcgcgtcggt gagcgacgcg acctggtcaa ggacgacccg
     3961 aagtcgggcc gcatcgtcgg cggcgcccgc aaagtcggcc gccagatcgg cgtcgagccg
     4021 ctccgggtgc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcggcggcg
     4141 cccgcaaagt cggccgccag atcggcgtcg agccgctccg ggtgccggcg atacccctcc
     4201 accagggtgc ggacgacctc ctcctggcgc cccatgacgg accgccgctc atcggtgaac
     4261 atcacgtagt gcgccgccac agccttgagc aacgagcatt cggcgcggac gtcgtcgggg
     4321 atcaccaggt cggccgcgaa ccgggtcagc ggcccggggc cataacgcgc tcgggtggcc
     4381 atctccacgg cggagaggaa ccgcccgatc agccggctgg tcatgtcctt gagcgccgca
     4441 aggtctgcac gcgtcccggc gaaactgtgc ggcaccgttc ccgtcgcgac gagacgctgg
     4501 gcggctgcca cgaacgcctc gaccggcaga tccggcgcgt atgccgtccg cgccagttcc
     4561 gcaaccaccc gcacctcggt gggtgaatag agcacccgcg ggtccacccg tccggaggcg
     4621 atggcgtcct cgacgtcgtg caccgaatag gcgacgtcgt ccgaccaatc catcacctgg
     4681 gcctccaggc accgcactcc cacgtcggcc ccctcccgga accactggaa gaccggcagg
     4741 tccgcggcat acaccccgta tttggctgtc tcgtaaggtc cttggccacg agcccaggga
     4801 tacttgacca cggcgtcgag gctggcccgg gtgaggttga gcccggccgg ccggccgtcg
     4861 tcgtggaccc gcttgggctc cagccgggtc aggacccgca acgtctgggc gttgccctcg
     4921 aagcccccgg cgtcactggc caattcatcc agcaccgact cgccatggtg gccgaacggc
     4981 ggatgcccga gatcgtgcgc caggcaggcc gtgtcgacga catcggcatc acacccgagc
     5041 gccgatccga actcccgccc gatctgggcg acctcgagcg agtgggtgag ccgcgtgcgg
     5101 atgaagtcgt ccgagccggg cgcgacgacc tgggtcttgg ccgacagccg ccgcaacgcc
     5161 gccgaatgca gcacccgggc ccgatcacgg gcgaagtcat cccggtcgga ctgcttgtgg
     5221 gcgggatcct cggcgaccca ccgctcgcgg tcgcgtgcgg catacgtcat ctgttgcgca
     5281 actccacgag gtgtccgacg acccccggaa cccagccgat ctcgccgttg aggaagcgcc
     5341 cgctgacgag gttggtgggc accagggcga agtagtcgct ctcggcgaac agcccgaggt
     5401 cggtgccgaa cgggtcctcg acggtgaccc aacgcagatc ggtcgtccgg gcgaggtgct
     5461 cgccgatagc gaccccccag cgcgcgatga cggggtcctg atcccccgcc ttggccgatc
     5521 gcaacccgcc gcgcgcaccc caggcggcgc actcggcgtc gtaacctgcc ccgatcgccg
     5581 cgatgtcgtc caggtcgatg cctgccgcct ccaactccgc cagacaccca tcaagcagtg
     5641 cccggtggtc ggcgcgcagg ggcaactcac ggggcccgag cgggcgcgac cagtcgtcgt
     5701 ccgcctcggc gtcgatcaac tccccctcgc cgaccccgtc gagcggggcg acgtcctcgg
     5761 acgggtcatc cttgcgccgc gagaacagtc ccatgccgcc agcctagggc cggggcggcg
     5821 cgggcggttc gcccagctcg acccgtgccc gccgcatcca tccggcttcg tcgccatagc
     5881 gcagcggagg gatcggcagg tatgccgcgc agctctcgat cagctggtcc atccgccggt
     5941 ctcgggttgc ctgttgtttg gcggagagca cccagtggat gacctgcttg cgataggacg
     6001 cgggtgctcg ctcccagaac gccggggcgg ccggggaggc ggctaggcgc gcggcatact
     6061 ccggcgggaa ggcgcccgag tcagcctgct cgtaggcgta gatccccgtg cggtcggccc
     6121 gcccgcgctc gtatgccgcg cgtcctgcgg gctgcatgcg gccctcggcc tcgagttgcg
     6181 ccacgagggc gacgttgatc ttgctccacg tcgagccgtt gcgacgcggc gtccaacgct
     6241 ggcggacggc atcggcaccc aggctctgca cgaccgagtc gatccagccg tagcacaggg
     6301 cttcgggcac cgcctcggcc caggtgagcc cccggggctc gacgtgcttc ttgcgcagac
     6361 ccatccacaa ctcgggcgcg gtctcgtggt tggcggccag ccagtcgcgg aactccgccg
     6421 ccgactcgaa gaaggtcgcg ggccgctccg gctggcccgc tggcggctct gtctgggtgg
     6481 tcatctcagc cgcccgagac gtcgagctcg gcctgcgcga cgagggcctg ttgctcgggc
     6541 gagaggtccg gcgagtccag ccaaccgtcg ggcagggcga cccgcttggg cgtcccggcg
     6601 cgcccgcgct gaccctcggc atcggcgccg ggccagggcg cgtcggcgtc gagcccgtcc
     6661 agcagccggt cgagggcggc cagcgagtcg accagcgcga ggtgctggcg catcgtccct
     6721 ccggcaggga atcccttgag gtaccaggcg atgtgcttgc ggatgtcgcg gcacccgcgg
     6781 ttctcgtcgt cgtagaactc cacgaggtat gccgcgtgcc gccgcatcgt gtcagccact
     6841 tcgccgaggg tcggctgcac tcgcagcgtc gacccctcga aggcggccgc gagatcggcg
     6901 aacagccacg ggcgcccgag acagccccgt ccgacaacga cgccgtcgca gccggtctcg
     6961 gcgaccatcc ggacggcgtc gtcggccgac cagatgtctc cgttgcccag caccgggatg
     7021 gtggtgaccg cctgcttgag ggcggtgatc gccggccaca cggcataccc ggagtagtgc
     7081 tgggcggcgg tgcgggcgtg caacgcgacg gcggcagcgc cctcctgctc ggcggcgacc
     7141 cccgcgtcga ggtaggtgag gtggtcgtcg tcgatgccga tgcgcatctt gacagtgacc
     7201 gggatgtccc ggcgagcgcc ctcagccacg acggcagcga cgatggcgcg gaagaggtcg
     7261 cgtttccacg gcagcgcgga gccgccgccc ttgcgggtga ccttggggac cgggcagccg
     7321 aagttgaggt cgatgtggtc ggcgcggtcc tcctgcacga ggatccgcgc ggctgcccga
     7381 gccgtcaccg ggtcgacgct gtagagctgc accgagcgcg gcgactcgtc ggcgtcatgc
     7441 tcgatgagcc gcatcgtctc ggcgttgcgc tcgacgagcg cgcgcgaggt gatcatctcc
     7501 gacacataga gcgcgcccgg cgcgtgctcg cggcacaacc gccggaaggc tcggttggtg
     7561 atgcccgcca tgggtgccag gaccacgggc gtcgacacgg tatgccgccc gatcggcagc
     7621 gcgggcagca cgggcagcac cgactgcgcg accgccgtca tcgggggctg cggcgcggtg
     7681 agggtggtct gggcggtcaa catggcggag ccattgtcgc aacaaccggc gtagctgagc
     7741 gaaaccgacg ctctgacgtc ggtttcgctc agctgaggga tcgccggtcc cgtcagtggt
     7801 gcagtcgggg cagccaggcg gggccctggt gcgctcggtc acccagcagc gcgaggacgg
     7861 ccggcagcag gacgccccgg acgaccgtcg catccagcag cacggcgacg gccagcccca
     7921 ctccgagttg cttgagctcg agcacggaca gcgtcccgaa caccgcgaac accccgacca
     7981 tgacggctgc ggcgctcgtc acgacgccgg ccgacctggc caccccgagt cgaacggcag
     8041 cccgcgcgtc gagcccggca tcgcgggctt cccgcacccg gctggtgacg aagacgtggt
     8101 agtccatcga caacccgaac agcacgacga acatcagcag cggcagccac gcggcaatcg
     8161 acccgatcga ggtgaagtcc agcagccgct cggcccacgt cccctggaag acgagcgtca
     8221 tcacgccgta ggccgcgccc accgaaagga ggttgaggcc gacggtggcc acggccaacc
     8281 agggcgagcc gaacgaaatg agcatgacga ccagcgtgag gatgagcacg aagcccacga
     8341 cccacggaag tcggctgtcc atccattggg ccaggtcggt cgctgccgct tcgcctccga
     8401 cgaccacctc gaccccaggt ctgccgcgca aggccgccgt gacctgcggc gccacctggg
     8461 tgcggacggc gtcgacgacc ccgggcagac gttcgtccga ggcgtcgagg gccacgccga
     8521 tcggcagcaa ggtgacggtg ccgtccttcg agacgctcgc ctgcggctgg acgcctgaga
     8581 cgtgcgcgag cgccgccgct cggggagcag ccgcggccaa ggcctctgcc accgcgggtg
     8641 cggcgctcgc cggggccttg acggtgagca agagcgcgac ggtcttggtc ggtgctgctg
     8701 ccgagagttg acggtatgcc gcgacgacgg ccaggtcctt gggcaggccc tcgacgtcgg
     8761 tcaacgcggt ccgcatcccc aaggcgggca ccgagagcgc gatgagcgcg accgcagcgg
     8821 tggcggccca ggccacggga cgagcaacga cgcggccggc gagcctcccc caccgcgact
     8881 cgaccgagcc agcgttggcc tggcgacgac gggtgcccgg cagtcgcagg gcgtcgaccc
     8941 ggtcaccaag cacggccagc agcgccggca gcaccgtggc agaggcgagg acggccactc
     9001 cgacgaccaa cgacgtgccg atggccaacg aggtgaacag cccgccagcg acgagcatcc
     9061 ccgacatggc caccatgacc gtgaggcccg agacgatgac ggctcgaccg gccgtcgctc
     9121 cggcaaggac gatggagtcc tcgacgctcg cgccggctgc ccgttcctcg cgggcgcggc
     9181 gcatgacgaa cagcgcgtag tcgacaccga ctgctagccc gatgagcagg accatgctct
     9241 gggtgttctg gtccacggtg accaggtggt tggagaccgc cgcggtgagg ccgagggcga
     9301 ccgcgacgct gccgatcccc agcacgagcg ggaccccagc agcgacaacc gcaccgaacg
     9361 cgatgagcag gatcaccagg gtcacgggga ggctgacgaa ctcggcgcgc tggaagtccc
     9421 tgcccaacgt ggcgtcgaac tcgcgggtga tcgaccctgg cccaatctgc cccacctgca
     9481 ggtccggtcg ggcggcaccc aggcgggtcg tcacggcgag cgacggggcg acgacctcct
     9541 cgggctgcgg cagggtggtg tctgtggccg aacgggccgc ggcgagttcg agggggatga
     9601 cgacgctgcg cccgtctgcc cccgggatcg ggtcacccac acgggccacg ccctccaccc
     9661 cttggtatgc ccccgccagc tcggctccca gcgccgcggc ctgctcgggc gcgagagagc
     9721 cggtgcggac cgtggcgatg atccgttcgg tcgggcggtc gccgaagtcg gcctgggaga
     9781 tgagggtctg ggcccgtgcc gactcgccga cgaggctctg ctcctgcggg gtggtgacga
     9841 tgccggtcga cagcagcatg accgacccga cgagcccgag ggtcgccagg gcgagcaccc
     9901 accatcggtg agtgacgcac caggcggcga ccgacgatcg gggcacccct gcaggtggct
     9961 cgacgcgtgg cttggtctgc ccgtgtgggg acgggggcat tcccgtgggg tccgccgcag
    10021 cggagtccgg tatcgcggac gaggagacgt gtgtggtcat gacttcgagg ctgtcgtctg
    10081 tcccgatccg ccacgacccg gtgtgacccc cacccgaccc ccacccgacc ctggcccaac
    10141 cccgagatcc ggttgaggag tccgtgcggg tcgcgggcct gcagctgacc gcgcggacag
    10201 ccccgccccg ctacacgctc gccaggcgaa ccgctatctc cggttgatcc ggatggtgtc
    10261 ctgcacggtt tgatcatcga gggtgggctg gtcggcgcgc gtcggcgtca actggggcca
    10321 gccgtcctca accgagaccg ttccctcgac cgcggcgatc gtgatctcga tgcgcccgtc
    10381 gatgaggacc aggtcgacct cactaccagc cgcgaggccc aaggcatcac gcacgtcggc
    10441 gggcaggacg acccgaccgg cggcatcgat ggtagttcgc atgagacctc tctaccatca
    10501 gcgggcgaca gcgaggccat ggcgagcagc agccgcagcc agcgccgcgg catacggcgg
    10561 ggtccactgg aagccctcgg acctgacctc cgcgacgatc ccctcgcgac ccaggacgtc
    10621 gctcaccgcg tcgccactga gcgagccggc cgccgcgagc gcctgggcga gagcgatgag
    10681 cgcaccacga tgcgcgcgca acaactcagc ggccctgtcc gcaagggagg ccaacagggc
    10741 ctccgcggtc ggctccgtca cggatgcgtc tgtggcatag gcgaattcga gcccatagtc
    10801 caacgcccac gagccgaagc caaggcgccg cacgctgtcc aggaccaacg acgtcgcccg
    10861 ctcccgatcg ctcgcccgac ccgccgaggc gtgttcggcg ccgaagatca gctcctcggc
    10921 cagcccacca gccagcgcca ccgtggccat gcgcagcatg ctgtcgcggg tctggtgcac
    10981 gtcgtgcgca cagaggaagc cgtcggcgtc acccgtcgcg tgcgccgtca gttgcagggg
    11041 cacgagtccg aacaacacgg catacgccac cgcatggccc gcctcgcagc gttccagttc
    11101 caggaggaag gccagccagc ccgcggaacc cagctcggcc tgggccgggc ccgcctgctc
    11161 cagcagccac agccgctcgc agctgcgggc ctcgatgagc gcggcgatga gcaggcggtc
    11221 cagcaggccc ttggaggcca gcttgtgcag ggcctgggcg taggggttgc ccaggtccgg
    11281 tcgcaac
//
LOCUS       DAOMZY010000075        11238 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5415_length_11238_cov_6.287669, whole genome shotgun sequence.
ACCESSION   DAOMZY010000075 DAOMZY010000000
VERSION     DAOMZY010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 11238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11238
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5415_length_11238_cov_6.287669"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..984
                     /locus_tag="PLK64_05935"
     CDS             139..984
                     /locus_tag="PLK64_05935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPV79315.1"
                     /translation="MDNDAQPLTPTAPATSAPAPADLGALFRLDARRAVVIGAGSGIG
                     QAIALGIAAHGAEVVCVDRDLAAAQTTAHQAGTGPGWGAMTAYQLDVLDTQAVTRAVE
                     DLGAVDILVVTPAVNVRKRLLSYTGEEFDSVVRLNLRAAFDLMTGFGRGMAQRGRGSI
                     IGCSSIRSLTTEPGQGVYAMTKAGLVMLLRTLAAELGPQGVRVNAIAPGVIDTPLTAP
                     IKADEQWYAAYGAKSILGRWGRPHELAGAAVYLASDAASYVTGTVLFVDGGWTAIDGR
                     FTPPS"
     gene            1195..3381
                     /gene="malQ"
                     /locus_tag="PLK64_05940"
     CDS             1195..3381
                     /gene="malQ"
                     /locus_tag="PLK64_05940"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HPV79316.1"
                     /translation="MPDPAHSVEAPTAPSLEQLQALAKAYGVGVDFWGFYGDLHAVSA
                     LTLTKVLAALGVDASTEQACERALADRAAQPWRQLLPPTVVMRENAPTLVRAHVHHGD
                     EVRVWVVDEAGAEWPVTQAEDNTSPHEVDGVTLGQASFWLPQELPLGWHLIRAESHGR
                     QAESTLIVVPNRLPTADALPAKAGRARTWGLMTQLYSVRSARSWGVGDVEDLADLAAI
                     SGAAGADWILVNPLHAAEPSPPMEASPYLPTTRRFFSPLYIRIEAIPESIYLTTAARR
                     HISLIWTQATARNHDASEVPRDAAYASKLEALQLVYAVPRSPSRDGRFAAFRAEHGRG
                     LEDFALWCAVRERSAEDAVIWEELRPHDEAAFALRQRLRDRIDFHCWLQWVLDEQLEA
                     AQASALRAGMALGIIHDLAVGVHPLGADAWVLRDVLAQNAEVGAPPDMYNQQGQNWSQ
                     PPWHPQRLAEAGYAPFRDMLRTILRHAGGIRVDHVLGLFRLWWVPLGLPADQGTYVYY
                     DHEALVGILCLEAQRAGAVVIGEDLGTFEPWVRDYLAGRGLLGTSILWFEYAADKAPL
                     APEAYRRGCLASVNTHDLPPTAGYLAGEHIALRDRLGLLTRSVEEEIAADREEQGRVL
                     DMCRRRGLLPEEGEAMPDGSEVGEQQIVEALYALLASAPSVMQGVALVDAVGERRVQN
                     QPGTDKEYSNWKIPLAGPEGAVVSVEELATNPRAQSLFRTVREALA"
     gene            complement(3430..3702)
                     /locus_tag="PLK64_05945"
     CDS             complement(3430..3702)
                     /locus_tag="PLK64_05945"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HPV79317.1"
                     /translation="MSHWVLEYRYTDPDARARVRPDHLAYMNGLHEDGRVVMGGPVGD
                     GGGALVVLRVDSEDEVRAIIAADPYTAAGVGADHVVRPWTVAIGGN"
     gene            complement(3778..4704)
                     /locus_tag="PLK64_05950"
     CDS             complement(3778..4704)
                     /locus_tag="PLK64_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79318.1"
                     /translation="MARRLEEVTRGDIDAIRYDDLSFDGSHGHVGVTLFMPSIRQLGI
                     KGEPEIISPYSAGWGEGLGAARASCEGLVRSANVMAATIEYPRKRVAVHEILQFRTEV
                     FGEVIRHIRERTMYDWATLVVAGYSRGTAPARMATVEHADVVDGVVLVAPTWFSQQVK
                     PRDLAARGLHESARGMAQAGWLDRLTLVGASVRLAQEMLAHPFELRNDIAAISQEGAA
                     DLQDVLDTGVRVGVVAGRQDELCQVDGIRTVVDRLPEPAAVMYREVDSDHFSYFLSPK
                     PLKVVAEVVTALGDFGDEAEVGDEAQAEGSGA"
     gene            complement(4815..6503)
                     /locus_tag="PLK64_05955"
     CDS             complement(4815..6503)
                     /locus_tag="PLK64_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPV79319.1"
                     /translation="MTSPLLRTIRESVIGDDQVLPGPYGPRRVTYADYTASGRALSFI
                     EDFIRDEVLPRYANTHTESSGTGLQTTRLREDARAIIRDAVGGDEDTVVIFTGSGCTG
                     AIDKLVGILGLRIPSQLEDAHHLAQHIPADARPVVFIGPYEHHSNELPWRETIADVVV
                     IPQDLDGHISLSVLRERLQEYAARPLKIGSFSAASNVTGIVSDSAAISTLLHEHGALS
                     FWDFAAAGPYIDIEMYSGGAGTTASNTAYKDAVFLSPHKFIGGPATPGVLVVRRELLT
                     NRVPAMVGGGTVAFVNPTEHRYLADPAHREEAGTPAIIESIRAGLVFQLKAAVGVDTI
                     RAQEERLLHRAVSAWTQEPTIQILGNLDAERLSIVSFVVKAPSGRYLHHNFVVAVLND
                     LFGIQSRGGCSCAGPYGHALLGIDLDRSHEFEAEIAHGCEGIKPGWVRVNFNYFVSDE
                     VTDYIVDAVRLIARDGWRLLPDYRFDNATGRWRHREGPVEPPLRLRDVGYAADGSFVF
                     PRHTARAAEGELAAYLIEGARILAERADDAGPQAEVSPGYDDLRWFELPAACMP"
     gene            complement(6662..7078)
                     /locus_tag="PLK64_05960"
     CDS             complement(6662..7078)
                     /locus_tag="PLK64_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPV79320.1"
                     /translation="MVAFISHTSIDCRNAYTLSVWWEGVLDYVPDPDDPNEPGHEECL
                     IRSRDGSHTLLFIEVPDDKVAKNRIHFDLRPSTGTRDEELERLLAHGARVLDDQRNPN
                     GTGWVVLADPEDNEFCILRSQAEVARHQALAAQPTD"
     gene            complement(7298..8878)
                     /locus_tag="PLK64_05965"
     CDS             complement(7298..8878)
                     /locus_tag="PLK64_05965"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV79321.1"
                     /translation="MSPPSTEHPLGTRRWWALATIALGVSLIIMDATIVSVALPVVIR
                     DLDLTANQAEWMNAIYSLTFAALLLTVGHLGDLRGRRLLFAGGMGLFMVASVFAGSAQ
                     SAEILIGARLLQGVGASAILTSTLATLNSIFRGRERAIAFAVYGATIGGMAAVGPLVG
                     GWLATDVSWRWAFWLNIPFGLLVLAGIWRVVPETRDPGAHSHFDLLGAALSALAMGGA
                     VFALIEGATYGWWRQPSGAPSPVPAVAAAAALSTVVFFVREGRARSAGRPAMIDVGLL
                     RLPTLRYGLIAAAVVAFGEFGLMFTLPLLLQGALGYSALGAGWLLVSLAIGAFLVSGA
                     TPHLTQAIGQRSVVRIGLALEAVAVAGIALTLSLTIASWAIAAWLFVYGVGVGMATAQ
                     LSSVIMSDVPPAQGGQASGLQSTVRQLGSALGIAVLGTLLVTSLGAGLQERLDASGMP
                     PAASAQVVEIVRGSAGAAIPSLAADPQGAGAAYLARAAMVDASRRTAGFAAAALALGV
                     LATLALPTPRRPDDDPHL"
     gene            8974..9567
                     /locus_tag="PLK64_05970"
     CDS             8974..9567
                     /locus_tag="PLK64_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPV79322.1"
                     /translation="MPRIEAPTVAEHNAMRRRQVIEAAADELRDGGVSSFTPAAVAKR
                     AGLARSSMYQYYPSTDALLGAAITTLLQRSRDRMIAATQAAGSPAERVTAYIEAAIAD
                     AQDGHGASPDLASVAMPEVCRDGVRVLHDQLREPLRSALRDAGVPDPDTAALLVQGLV
                     NAAASAINHGVPLERISAATVDLVLRGTGLLAQADAV"
     gene            complement(9580..10872)
                     /locus_tag="PLK64_05975"
     CDS             complement(9580..10872)
                     /locus_tag="PLK64_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HPV79323.1"
                     /translation="MAKHPAPLPLDEQTVPGGAAEASSAAPSRRSFLGVGAALAGGAG
                     AGLVGYGLGNSQTGVAPSAVAAASSEGAAPDAPWAGSVIPFIGAHQAGISTPPQAHAT
                     FVALNLTPGVGQDGLRRLLRQWTNDASRLQRGIPVLGDPQPDLASPPSRLTITVGLGR
                     GALAEAGLLERAPAWLAPLPAFSIDKLEQRWNEGDVLAQVCAEDPIVLAHAVRALLLS
                     ATGIATVHWQQRGFRRPVTSTSAMRNLMGQVDGTVQPVAAELEQSVWIGADGPDWLRG
                     GTSLVLRRIRMDLDTWDALDPQAKEQVIGRRMTSGAPLTGTKETDVPDLAATRDGLPV
                     IPEFAHIRHAAVTTAGQKILRRPYNFDDTPAAGSSAEAGLLFAAFQADPVRQFVPMQQ
                     RLATGDLLNFWTTPIGSAVFAILPGPAEGEILGQALVG"
     gene            complement(10865..>11238)
                     /locus_tag="PLK64_05980"
     CDS             complement(10865..>11238)
                     /locus_tag="PLK64_05980"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HPV79324.1"
                     /translation="HLLSASSPISPMMQLHETVMGTGGAMAMQEKPGGFTIPAGGSHE
                     FKAGGDHVMFMGVSAAVKSGTTVTFTLAFEDGSTLPVTAEVRTFTGAKETYVPGATTG
                     TNSMPGMTPTPGTSGTTASHG"
BASE COUNT         1741 a         3870 c         3999 g         1628 t
ORIGIN      
        1 ggacgaaacc ccggcctgac ttccccgaat ccctcacgct cgccgatgat ctgggcgaac
       61 ccgggggtct ggaccccaaa tccctcacac tcgccggcga tctctggggc ccggaggctg
      121 gctgacacaa tcgagcgcat ggacaacgac gcccagcccc tcacaccgac tgcgcctgcc
      181 acatcggcgc ccgccccggc tgacctcggt gccctcttcc gcctggacgc tcgccgcgcc
      241 gtcgtcatcg gcgccgggtc aggcatcggg caggccatcg ccctcggaat cgcggcccat
      301 ggcgccgagg tggtctgcgt cgaccgcgac ctggccgcag cgcagacgac cgcccaccaa
      361 gccggcacgg gccctgggtg gggggcgatg acggcatacc aactcgacgt cttggacacg
      421 caggcagtga ctcgcgcggt cgaggacctc ggggccgtcg acatcctcgt cgtcacgccg
      481 gcagttaacg tccgcaaacg gctgctgagc tacacgggtg aggagttcga ctcggtcgtg
      541 cggctcaacc tgcgagccgc gttcgacctg atgaccgggt tcggacgcgg gatggcgcag
      601 cgcggtcgcg gcagcatcat cggctgcagc tcgatccgga gtctgacgac cgagccgggc
      661 cagggcgtct acgcgatgac caaggctggt ctcgtcatgc tcctgcgcac cctggctgcc
      721 gagctcggcc cgcagggagt tcgcgtcaac gccatcgctc ccggggtcat cgacacgccc
      781 ctcaccgccc cgatcaaggc cgacgagcag tggtatgccg cctacggcgc caagagcatc
      841 ctcggacggt gggggaggcc gcacgagttg gcaggcgccg cagtctattt ggcctcggac
      901 gcggcgagtt acgtcacggg gacggtccta ttcgtcgacg gcggatggac ggcgatcgac
      961 ggccggttca cgccgccgag ctgagtcgga tcgatccccc ttcgggcgtg agagcgccca
     1021 tgaagtcgtc gcaggtggcg tgatcacgca cgtagaggcg acttcatggg cggcttcacg
     1081 cccggccacg tcgcacccag gcagtcccga tccggcgggg cgacacggca tacccctggg
     1141 gagtggaaga atcgccccgg acgtttcccc cgcacccccg acgaggcagg accgatgccc
     1201 gaccccgccc actccgttga ggcgcccacg gccccctcgc tggaacagtt gcaagcactg
     1261 gcgaaggcct atggcgtcgg cgtcgacttc tgggggttct acggcgacct ccatgcggtc
     1321 tcggcgctga ctctcaccaa ggtcctggct gcccttggcg tcgatgcctc gaccgagcag
     1381 gcctgcgaga gagccctggc cgatcgcgcc gcccaaccct ggcgccagct gctccccccg
     1441 acggtcgtca tgcgcgagaa cgcgccgacc ctcgtccggg cgcacgtcca ccacggcgac
     1501 gaggtgcgcg tctgggtggt cgatgaggca ggcgccgagt ggccggtcac gcaggccgag
     1561 gacaacacct cgccccacga ggtcgacggg gtgaccctgg ggcaggcgag cttctggctg
     1621 cctcaagagc ttcccctcgg gtggcacctc atccgcgccg aaagccatgg ccgccaggcc
     1681 gagtcgaccc tcatcgtcgt ccccaaccgg ctgccgaccg ccgacgccct tccggctaag
     1741 gccgggcgcg cccgcacgtg gggcctgatg acccagctct actccgttcg gtcggcccga
     1801 tcgtggggcg tcggcgacgt cgaggacctg gccgacctcg cggcgattag cggtgcggcc
     1861 ggcgcggact ggatcctcgt taaccccctg catgcggccg agcccagccc tcccatggag
     1921 gcctcgccct acctgccgac gacgcggcgc ttcttcagcc cgctctacat ccgcatcgag
     1981 gcgatccccg agtcgatcta cctcacgacg gccgcccgcc gacacatctc gctcatctgg
     2041 acgcaggcga ccgcccgcaa tcacgacgca tccgaggtgc cgcgcgacgc ggcatacgcg
     2101 agcaagctcg aggccctcca actcgtgtat gccgttccgc gcagtcccag ccgcgacggc
     2161 cggttcgcgg cgttccgggc tgaacacggc cggggtctgg aggatttcgc gctgtggtgc
     2221 gccgtgcgcg agcgcagtgc cgaggacgcg gtcatctggg aagagttgcg cccccacgac
     2281 gaggcggcct tcgccctgcg gcagcgcctg cgggaccgga tcgacttcca ctgctggctg
     2341 cagtgggtcc tcgacgagca gttggaggcg gcccaggcca gcgcgctgcg ggcgggaatg
     2401 gcactgggga tcatccacga cctcgccgtg ggcgtgcacc ccctcggcgc cgatgcctgg
     2461 gtgctgcgcg acgtcctcgc ccagaacgcc gaggtcggcg caccgccgga catgtacaac
     2521 cagcagggcc agaactggtc acagccgccg tggcatccgc agcggctggc cgaggcgggt
     2581 tacgcgccct tccgggacat gctgcgcacg atcctgcggc acgccggggg gatccgtgtc
     2641 gaccatgtcc ttgggctgtt ccggctctgg tgggttccgc tggggctgcc agccgaccag
     2701 gggacctacg tctactacga ccacgaggcc ctcgtcggca tcctctgcct ggaggcgcaa
     2761 cgcgccggcg ccgtcgtcat cggggaggac ctgggcacct tcgagccgtg ggtgcgcgac
     2821 tacctcgcgg ggcgcggcct gctcggcacg tcgatcctgt ggttcgagta tgccgccgac
     2881 aaggcgccgt tggcccccga ggcctatcgg cgcgggtgcc tcgcatcggt caacacccat
     2941 gacttgccgc cgaccgccgg ttatctcgcc ggcgagcaca tcgcgctgcg cgaccgcctc
     3001 gggctgctaa cccgttcggt cgaggaggag atcgccgccg accgcgagga gcaggggcgg
     3061 gtgctcgaca tgtgtcgacg ccggggcctg ctgccggagg agggcgaggc gatgcccgat
     3121 ggcagcgagg tcggcgaaca gcagatcgtc gaggcgctct atgccctcct ggcgtcggct
     3181 ccctcagtga tgcagggggt cgctctcgtc gacgccgtgg gcgagcgccg ggtgcagaac
     3241 cagccgggca ccgacaagga gtactccaac tggaagatcc cgctggccgg tcctgaaggc
     3301 gccgtcgtca gcgtcgagga gttggcgacc aacccgcgcg cccagtcgtt gttccggacg
     3361 gtccgcgagg cgcttgcctg agtcggccgt cagtcggcag ccggacgccc cctgctgcga
     3421 gcgacgctgt tagttgccgc cgatggcgac ggtccacggg cgcacgacgt gatccgcgcc
     3481 caccccagcg gccgtgtagg gatcggccgc gatgatggcc cggacctcgt cctccgaatc
     3541 gacgcgcagc accaccagcg cgccgccgcc gtcgccgacc gggccgccca tgacgacgcg
     3601 gccgtcctcg tgcaggccgt tcatgtaggc caggtgatcc ggccgcaccc gggcgcgtgc
     3661 gtcaggatcg gtgtagcggt attccaggac ccaatggctc atggcggcag catgccagaa
     3721 ctggtgcgag cccacgcgcg ggcccgcagg ctcgtgcggg cccacgtcgc gcagccctca
     3781 ggctcctgaa ccctcggcct gcgcttcgtc cccgacctcg gcctcgtccc cgaagtcgcc
     3841 gagcgccgtg acgacctcgg cgacgacctt gaggggtttg ggggagagga agtaggagaa
     3901 gtggtcggag tcgacctcgc ggtacatcac tgcggccggc tcggggaggc gatcgacaac
     3961 ggtccggatc ccgtcgacct ggcagagctc gtcctggcgg ccggcgacga caccgaccct
     4021 gacacccgtg tcgaggacgt cctgcaggtc ggccgcgcct tcctgggaga tggcggcgat
     4081 gtcgttgcgc agctcgaagg ggtgggccag catctcctgg gcgaggcgga cactggcgcc
     4141 gacgagggtg agccggtcga gccacccggc ctgggccatc ccgcgggcgc tctcgtgcaa
     4201 cccgcgcgcg gcgaggtcgc ggggtttgac ctgctggctg aaccacgtgg gggcaacgag
     4261 gacgacgccg tcgacgacgt cggcgtgctc gacggtcgcc atgcgcgccg gggccgtgcc
     4321 gcgcgagtag cccgcgacga cgagggtggc ccagtcgtac atcgtccgtt cgcggatgtg
     4381 gcggatgacc tcgccgaaga cctcggtgcg gaactgcagg atctcgtgga cggcgacccg
     4441 tttgcggggg tattcgatcg tggcggccat gacgttggct gaccgcacca ggccctcgca
     4501 cgatgcccgg gccgcgccga ggccttcgcc ccagccggcc gagtaggggc tgatgatctc
     4561 gggctcgccc ttgatgccga gttggcggat cgagggcatg aacaaggtga cacccacgtg
     4621 cccgtgagag ccgtcgaaac tcaggtcgtc ataccggatg gcgtcgatgt cgccccgggt
     4681 aacttcctct agtcgccgtg ccatttgcca atggtgccct tcactaccga tgagtagcgt
     4741 ctttggttgc gctgatcacc atcacgattt gggccacagt atgcccacac ctgagtatga
     4801 ccacgcgtcg cggctcacgg catgcatgcc gctggcaact cgaaccaacg caggtcgtca
     4861 taacccggcg agacctcggc ctgtggcccg gcgtcatccg cccgctcggc gaggatccgg
     4921 gcaccctcga tcaggtatgc cgcgagctca ccctccgcgg cccgcgcggt gtggcgcgga
     4981 aagacgaaac tgccgtccgc ggcatacccc acgtcccgca gtcgcagcgg cggctcgacc
     5041 gggccttcgc ggtggcgcca ccgcccggtc gcgttgtcga accgatagtc cgggagcagc
     5101 cgccacccgt cgcgggcgat cagtcggacc gcgtcgacga tgtagtcggt gacctcgtcg
     5161 gagacgaagt agttgaagtt gactcgcacc cagcccggct tgatgccctc gcagccgtgg
     5221 gcgatctcgg cctcgaactc gtgcgaacga tccaggtcga tcccgagcag ggcgtggccg
     5281 tagggtccgg cgcacgaaca accgccgcgg gactggatgc cgaacaggtc gttgaggacg
     5341 gcgacgacga agttgtggtg caggtagcgg ccgctcggag ccttgacgac gaacgagacg
     5401 atcgagaggc gctcggcgtc gaggttgccg aggatctgga tggtcggctc ctgggtccag
     5461 gcggacacgg cgcggtgcaa gagccgctcc tcctgggcgc ggatcgtgtc gaccccgacg
     5521 gccgccttga gttggaacac caggccggcg cggatcgact cgatgatcgc tggggtgccc
     5581 gcctcctcgc ggtgggcggg gtcggcgagg taacggtgct cggtcgggtt gacgaaggcg
     5641 accgtgccgc cgccgaccat ggcggggacc cggttggtga gcaactcgcg gcgcacgacc
     5701 agcacgccag gggtggcggg gccgccgatg aacttgtgcg gcgacaggaa gaccgcatcc
     5761 ttgtatgccg tgttgctcgc cgtggtcccg gcgccgccgg agtacatctc gatgtcgatg
     5821 taggggccgg ccgcggcgaa gtcccagaag gacagtgcgc catgctcgtg caacagggtg
     5881 ctgatcgcgg cgctgtccga gacgatgccg gtgacgttgg aagcggcgga gaaggacccg
     5941 atcttcaggg gccgagcggc gtactcctgc agtcgttctc gcaggacgga cagggagatg
     6001 tggccgtcga ggtcctgcgg gataacgacg acgtcggcga tcgtttcgcg ccacggcagc
     6061 tcgttggagt ggtgctcgta gggcccgatg aagacgaccg gtctggcgtc ggccgggatg
     6121 tgctgcgcga ggtggtgggc gtcctcgagt tggctgggga tgcgcaggcc gaggatcccg
     6181 accaacttgt cgatcgcgcc ggtgcagccc gagccggtga agatgacgac cgtgtcctcg
     6241 tcgccgccga cggcgtcccg gatgatggct cgcgcgtctt cacgcagccg ggtcgtctgc
     6301 aggccggtgc cggaagactc ggtgtgcgtg ttggcgtaac gggggagcac ttcgtcgcgg
     6361 atgaagtcct cgatgaacga cagggcgcgg ccggaagcgg tgtagtcggc gtaggtcacc
     6421 cgacgcgggc catagggacc cggcaggact tggtcgtcgc cgatgacgct ctcgcggatg
     6481 gtgcgcagca aagggctggt cacggcgaca ggatagcgcc aggttttggc tttccgtcaa
     6541 gccataaccc cgcattatgc agccctctac ccggagatag cgctcgcacg gtcatgtttc
     6601 agggtctgaa agccgtaatg acccccataa atgaccgtgc gagcgctatc tccgtggtgg
     6661 gtcagtcggt gggctgggcg gcgagggctt ggtggcgggc gacctcggcc tggctgcgca
     6721 ggatgcagaa ctcgttgtcc tcgggatcgg ccaggacgac ccaacccgtc ccgttcgggt
     6781 tccgctgatc gtcgagcacc cgggctccgt gggcgagcag gcgctcaagc tcctcgtcgc
     6841 gcgtgccggt gctgggccgc aggtcgaagt ggatccggtt cttggcgacc ttgtcgtcag
     6901 gcacctcgat gaacagcaag gtgtggctgc catcgcggga ccggatgagg cattcctcgt
     6961 gccccggctc gttcggatca tcggggtccg gcacgtagtc cagcacgcct tcccaccaga
     7021 ccgacagggt gtaggcgttg cggcagtcga tgctcgtgtg cgagatgaag gcgaccatct
     7081 cggcagtgtc gcagcctccc ggagcccgcc gcgagggatt tgtggctgca acgccccctg
     7141 aggcgcccac cccccacaac ggagatagcg ctcgcatggt catgtgtggg ggctggaaac
     7201 ccctgaacgg acggaaagat gaccgtgcga gcgctatctc cgggtctgaa gtcgggtgtg
     7261 aagggggagg aggggtgggg gcaggggagg gagtcggtta gaggtggggg tcgtcgtcgg
     7321 ggcggcgtgg tgtgggcagg gcgagggtgg ccaggactcc gagggccagt gcggcagcgg
     7381 cgaacccggc cgtccgccgg ctggcgtcga ccatggccgc tcgagcgagg tatgccgcac
     7441 cagcaccctg ggggtcggcg gccagggagg ggatggccgc cccggccgag ccacgcacga
     7501 tctcgacgac ctgcgcggag gcggcgggcg gcatacctga ggcgtcgagg cgctcctgca
     7561 gaccggcgcc gagcgacgtg accaggagcg tgccgagcac cgcgatcccc agggccgacc
     7621 cgagttggcg caccgtcgac tgcagcccgg acgcctgccc gccctgcgcc ggcggcacgt
     7681 cgctcatgat gactgaactc agctgcgcgg tcgccatgcc gaccccgacc ccgtagacga
     7741 acaaccacgc ggcgatggcc caggacgcga tcgtcagaga cagcgtgagg gcgatgccgg
     7801 cgaccgcgac cgcctccaag gccaagccga tccgaacgac gctgcgttgt ccgatcgcct
     7861 gcgtgaggtg cggggtcgcg ccggagacga ggaaagcgcc gatggcgagg ctgaccaaca
     7921 accaccccgc accgagtgcg gagtagccga gggccccctg cagcaacagc ggcagggtga
     7981 acatcagccc gaactcgccg aacgcgacga cggccgccgc gatgaggccg tagcgcagcg
     8041 tcggcaggcg cagcagcccg acgtcgatca tcgccgggcg cccagccgag cgggcccgcc
     8101 cttcacgtac gaagaacacc accgtcgaca gcgcggcagc ggcggctacg gcgggcaccg
     8161 gtgacggggc gccggacggc tggcgccacc agccgtacgt cgcaccttcg atgagggcga
     8221 agaccgcccc gcccatcgcg agagccgaca gcgccgcgcc caggaggtcg aagtggctgt
     8281 gggcgccggg gtcgcgggtc tccgggacga cgcgccagat cccggccagg acgagcagac
     8341 cgaaggggat gttgagccag aacgcccagc gccaggacac gtcggtggcc agccatcccc
     8401 cgacgagcgg tccgacggca gccataccgc cgatggtggc gccgtagacg gcgaaggcga
     8461 tcgcgcgctc ccggccgcgg aagatcgagt tgagggtggc cagggtgctc gtgaggatcg
     8521 ccgacgcgcc gacgccctgc agcaggcgcg caccgatgag gatctcggcg ctctgggcgc
     8581 tgccggcgaa caccgaggcc accatgaaca ggcccatgcc gcccgcgaag agcaggcgtc
     8641 gcccgcgcag gtcgccgaga tgccccaccg tgagcaggag agcggcgaag gtcagggagt
     8701 agatggcgtt catccactcg gcctggttgg cggtcaggtc gagatcgcga atgaccacgg
     8761 gcagcgcgac ggagacgatc gtcgcgtcca tgatgatgag cgacacgccg agggcgatgg
     8821 tcgccaaggc ccaccatcgg cgggtgccga gcggatgctc ggtcgacggc ggactcattg
     8881 ccctgctcac gcgcctcaca gacgatagtt tatcgacacc tctgtcggaa tcacaacagg
     8941 aacgacactc gcgcaggcca atacactctg ggcatgccgc gcatcgaggc ccccaccgtc
     9001 gctgagcaca acgccatgcg tcgtcgccag gtcatcgagg cggcggccga cgagttgcgc
     9061 gacggcggcg tctccagctt caccccggcg gccgtcgcca aacgagcggg cctggcccgc
     9121 agcagcatgt accagtacta cccgtcgacc gatgccctcc tcggggccgc catcacgacc
     9181 ctgctgcagc gcagccggga ccggatgatc gcagcgaccc aggccgccgg gtcgccggcc
     9241 gagcgggtca cggcatacat cgaagcggcg atcgccgacg ctcaggacgg acacggcgcc
     9301 tccccggatc tcgcgagcgt cgccatgccc gaggtatgcc gcgacggcgt gcgcgtgctg
     9361 cacgaccagt tgcgcgagcc gctgcggtcg gcgctgcgcg acgccggggt tcccgacccc
     9421 gacaccgcgg ccctgctggt gcaggggctg gtcaacgccg ccgcgtcggc gatcaatcac
     9481 ggcgtcccgc tcgagcggat ctccgcagcg accgtcgatc tcgtgctgcg cggaacgggc
     9541 ctgctcgccc aggccgacgc cgtctgactt cgccccccgt cagcccacga gcgcctgccc
     9601 aaggatctcg ccctcggccg gcccgggcag gatcgcgaac acggccgatc cgatcggggt
     9661 cgtccagaag ttgagcaggt cgcccgtggc caagcgttgc tgcatcggca cgaactgccg
     9721 gaccgggtcc gcctggaagg ccgcgaagag cagccccgct tcggcgcttg agcccgccgc
     9781 aggcgtgtcg tcgaagttgt agggacggcg caggatcttc tgcccggccg tggtgacggc
     9841 cgcgtgcctg atgtgggcaa actccggaat gaccggcagc ccgtcgcgcg tggctgccag
     9901 gtcggggacg tcggtctctt tggtgcccgt cagcggtgcc ccactggtca tccgccgacc
     9961 gatgacctgc tccttggcct gcgggtcgag cgcgtcccac gtgtctagat ccatccggat
    10021 ccgccgcagc accagtgaag tgccgccgcg cagccagtcg gggccgtccg cgccgatcca
    10081 cactgactgc tcaagctcgg ccgcgaccgg ctgcaccgtc ccgtcgacct ggcccatgag
    10141 gttacgcatc gcgctcgttg aggtgactgg gcgccggaac ccccgctgtt gccaatggac
    10201 tgtggcgatg cccgtagcag acaacagcaa ggcccggacg gcatgtgcga ggacgatcgg
    10261 gtcttcggca cacacctgag cgagcacgtc tccttcgttc cagcgttgct cgagcttgtc
    10321 gatcgagaag gcgggcaggg gggcgagcca tgccggggcg cgttccagca accccgcctc
    10381 ggcgagtgct ccgcgcccca accccacggt gatcgtcagc cgggaggggg gcgacgcgag
    10441 atcgggctgg ggatccccca gcacgggtat gccgcgctgc agccggctcg cgtcgttggt
    10501 ccactggcgc agcagccggc gcaggccgtc ctggcccacc cctggagtga ggttgagcgc
    10561 gacgaaggtc gcgtgcgcct gaggcggggt gctgatccca gcctgatgcg cgccgatgaa
    10621 ggggatcacc gaccccgccc atggcgcgtc gggagccgcg ccctcgctgg aggctgcggc
    10681 gacggcagag ggagcaacgc cggtctggct gttccccaag ccgtagccga cgagcccggc
    10741 tccggcgccg ccggccaagg ccgctccgac gccaaggaac gaccgccgcg acggcgcggc
    10801 cgaggaagcc tcggccgcac cgcccggcac cgtctgctcg tccaggggaa gcggagccgg
    10861 gtgcttagcc atgagaagcc gtcgtcccag acgtgcccgg ggttggtgtc atgccgggca
    10921 tggagttcgt gcccgtcgtt gcgccgggga cgtaggtctc cttggcgcct gtgaaggtcc
    10981 gcacctctgc agtcaccgga agggtggagc cgtcctcgaa ggcgagggtg aaggtcactg
    11041 tcgtccccga cttcaccgcc gcgctgacgc ccatgaacat cacgtggtca ccgccggcct
    11101 tgaactcgtg cgagccgccg gccggaatgg tgaaaccgcc cggcttctcc tgcatggcca
    11161 tcgcgccgcc ggtgcccatg accgtctcgt gcaactgcat catcgggctg atcgggctgc
    11221 tcgcggacag gaggtgga
//
LOCUS       DAOMZY010000076        11206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5436_length_11206_cov_6.089768, whole genome shotgun sequence.
ACCESSION   DAOMZY010000076 DAOMZY010000000
VERSION     DAOMZY010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 11206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11206
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5436_length_11206_cov_6.089768"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PLK64_05985"
     CDS             complement(<1..226)
                     /locus_tag="PLK64_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79325.1"
                     /translation="MSLLDRVRSTVHASRGTVDALRPQTPTWRVWASGAVAVGVPWLA
                     TIGMAVLVWAVTPASDAPWGQVLGIASAGWF"
     gene            complement(349..1242)
                     /gene="sucD"
                     /locus_tag="PLK64_05990"
     CDS             complement(349..1242)
                     /gene="sucD"
                     /locus_tag="PLK64_05990"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HPV79326.1"
                     /translation="MAIFLTRDSKVIVQGMTGSEGMKHTTRMLASGTQVVGGVNPRKA
                     GTTVDFPGGVQVPVFGTVAEAIEATGADVSVIFVPAAFTKAAVVEAVDAKIPLAIVIT
                     EGVAVKDTAEFFEYAQDAGTTRLIGPNCPGLITPGQANAGIIPADISGAGRIGLVSKS
                     GTLTYQMMFELRDFGFSTAVGIGGDPIIGTTHIDCLQAFQDDPDTDAIVMIGEIGGDA
                     EERAAAYIAEHVTKPVVGYVAGFTAPEGKTMGHAGAIVSGSKGTAQAKAEALEAAGVR
                     VGKTPSATAQLMREIMQGLTR"
     gene            complement(1277..2479)
                     /gene="sucC"
                     /locus_tag="PLK64_05995"
     CDS             complement(1277..2479)
                     /gene="sucC"
                     /locus_tag="PLK64_05995"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HPV79327.1"
                     /translation="MDLYEYQARDMFEAHGVPVLGGEVAVTPEEAFAAAQRVGAKSGG
                     VVVVKAQVKTGGRGKAGGVKVAKSPEEAQALAAQILGMDIKGHTVKKVMIAEGAKIAE
                     EYYFGLLLDRTNRTYLAMCSKEGGMDIETLAVEHPEKLARIAVDAIDGLDEAKAREII
                     AAAGFDEADVPALVNVLVTLGDVYAGEDATLVEVNPLVKMEDGQIVALDGKVTLDDNA
                     RFRHPEHEVLIDHSVTDPLEVLAIEKSLNYVKLDGNIGIIGNGAGLVMSTLDVVAYAG
                     EEFTARDGSHPRPANFLDIGGGADANVMANGLDVIFEDHQVKAVFVNVFGGITACDQV
                     ADGIVKAYEILASHGKSPKPLVVRLDGNNAEKGREILDGAGLPLLERVDTMDGAARRV
                     AELAAAAV"
     gene            complement(2668..3684)
                     /locus_tag="PLK64_06000"
     CDS             complement(2668..3684)
                     /locus_tag="PLK64_06000"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HPV79328.1"
                     /translation="MIRPAFAIGLSAAVLVACSSTPSASAPSNPTTTTAGSISTGAAA
                     PTNTLPAVAVGTPLLGPGPVLAVKIDNTSPARPRIGVDAADIVYVEPVEAGLTRLLAI
                     YASTLPPEVGPVRSARESDAVLLGNYGRVAFAFSGASPITTSEIVTGPQVNVSMDSGG
                     AGYRRASDRKAPYNVIGTPAALVARAGGSAPAGDIGFRTGPGAPGGRPATSLATAYPL
                     ARVAFTWDATLGRYLVTTDGRPEVTAAGTPVSAASILVQMIRTHPSNNRDVNGSATPV
                     LELLGTGEAKVLRGGAVYDATWERATNASPTRLTGASGAEVTLAAGPLWVLLVPVGQG
                     VTIS"
     gene            complement(4021..4422)
                     /locus_tag="PLK64_06005"
     CDS             complement(4021..4422)
                     /locus_tag="PLK64_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HPV79329.1"
                     /translation="MSDSPLRVVVAKPGLDGHDRGAKVVARALRDAGMEVVYTGLHQT
                     PEQIVEAAIQEDADAVGLSVLSGAHMTLFAKVVELLKVRDAADIVVFGGGIIPDDDLL
                     ALQKLGVAKVFTPGAATHEIVDWVRANVHGH"
     gene            4698..5534
                     /locus_tag="PLK64_06010"
     CDS             4698..5534
                     /locus_tag="PLK64_06010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPV79330.1"
                     /translation="MTDAKYRGRHRSRRGAGLSPRIVGTGLVLPTTATLAVVSVTGGA
                     PMALTSTTLAAGASTVDDATTDRAELTQDKSTDSTLLARGVQDRVARDSERVRVDTVA
                     QENAKRAATEASLAANDVPVQVSLVGAPGSKLAASAASLKGTNLGSKAWVNPLGGNYS
                     LSSNYGWRWGRLHSGQDMACPTGTPVRSISSGVVTFSGWAGNLGYKVEILHWDGTVSW
                     YGHNSKLRVSEGQRVEPGQLIALAGSTGNSTGPHVHLEIHPNGGNAVPPKRWLAARGV
                     NI"
     gene            complement(5610..8168)
                     /gene="pcrA"
                     /locus_tag="PLK64_06015"
     CDS             complement(5610..8168)
                     /gene="pcrA"
                     /locus_tag="PLK64_06015"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HPV79331.1"
                     /translation="MGTLFDELGLSGFGEPEPSVPARPHPHAPSLADVRPSATPARRQ
                     DDVDEAGLPLWAQEGSGYAAPDAGGRHTGGRGAGDPERFLVGLNPQQRAAVVHEGSPL
                     LIIAGAGSGKTRVLTHRIAYLLAERGVQPGQVLAITFTNKAAAEMRERVESLVGARSR
                     AMWVMTFHSACVRILRREIEKFGLKSSFSIYDAADSQRLMAMVLRDMDLDPKRYNPRS
                     FSHQVSNLKNELIDEETYTRQVSGDEGTHHERTIAQAYVAYQRRLRQANALDFDDLIM
                     TTVHLLQSFPDVAEHYRRRFRHVLVDEYQDTNTAQYQLVRELVGVTSDAAGEGGSGAS
                     GGSGAGGERAAYAIPPAELVVVGDADQSIYAFRGATIRNIVEFEQDYPNARTILLEQN
                     YRSTQTILRAANSVIARNPDRRPKNLWTDSGDGARIIGYVGDNEHDEAAFIARTIDRL
                     GDEHGVKPKDVAVFYRTNAQSRAIEEVFVRVGLPYKVVGGTRFYERKEVKDALAYLRV
                     ISNPTDAVNLRRILNTPRRGIGERAEACVAQLADRERIPFVAALGRAVDAPGIATRSV
                     AAIEAFTRLLEDLRTVFEGGSGVAGLLEAVLEQSGYLRELRNSPDPQDETRVENLAEL
                     VAVAQEFDEGRAETGEPASLEDFLEQVSLVADADEIPDPEADAGGVVTLMTLHTAKGL
                     EFPVVFVTGLEDGTFPHLRSLADPKELEEERRLAYVGITRARERLHLSRAAVRSAWGQ
                     PQFFPGSRFLDEIPEELVAWEREAAASDSARFGRRSSEPAGATLAARPGVRSPGNRAV
                     PVLVAGDRVSHDSFGLGTVLRLEGEGERTLAHVDFGASVGVKRLLLRYAPVEKL"
     gene            8238..9224
                     /locus_tag="PLK64_06020"
     CDS             8238..9224
                     /locus_tag="PLK64_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HPV79332.1"
                     /translation="MTDARALRVTQHGGPEVLAVQTVDVPEPGPGQVRVRVEAAGINF
                     IDVYQRQGVYPGPTPFTPGMEGAGIVESVGADVDLLVGSRVAWAMARGAAAELAVLPA
                     ASAVPVPNDITAEQAAAAMLQGMTAHYLTTSTYAVQPGDFVLVHAAAGGVGQWLVQVC
                     SLAGATVIATAGSEAKLAIARELGAAYTLDYTAYATPAELAAAVREATGGVGVAVAYD
                     GVGKATFDASLGSLRPRGMLALFGGASGQVPPFDLQRLNAAGSVFVTRPTLAHYVADR
                     HELLWRAGEVFQTLASGRVRMDIGGVYALEDAAAAYEALEGRATTGKLIFQI"
     gene            9339..10112
                     /locus_tag="PLK64_06025"
     CDS             9339..10112
                     /locus_tag="PLK64_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPV79333.1"
                     /translation="MSDNVRVTREDSTTTITLARPEKRNALSAAMMTDLTAALAEAGA
                     SDTLAVVIAADGPVFSAGHNFGDLARADLRAARELFLLCTTMMDTVQGIPQPVIAKVH
                     ALATAAGCQLVASCDLAIAADTAGFALPGGKGGLFCHTPLVAVSRNVGRKRALEMALT
                     GDVIDAATAADWGLINRAVPADDLDAAVSDLVARVTRGSAMGRSIGKRTFYAQVGLEQ
                     RSAYDLAVEVMAAAALTDDAQEGIAAFLAKRHAAYTSLP"
     gene            10155..10373
                     /locus_tag="PLK64_06030"
     CDS             10155..10373
                     /locus_tag="PLK64_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79334.1"
                     /translation="MRTLYLRNVPDEVGDALEELASRASMSVSAYAVRELSAAVAFRS
                     NADLLWTQPQVEVDIDDVVAAVRSGRDR"
     gene            10370..10771
                     /locus_tag="PLK64_06035"
     CDS             10370..10771
                     /locus_tag="PLK64_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPV79335.1"
                     /translation="MIVVDTSVVIAALLGQDPARQALVGRRLVAPHLIDAEVAHALRG
                     LVLGVKVTADDGERLLSVWQHLAVDRLPMTGLLPRAWALRDALTAYDAMFVAAAEALH
                     VPLVTCDRRLAHAGAARCTVEIVPGASSMPT"
     gene            10768..>11206
                     /locus_tag="PLK64_06040"
     CDS             10768..>11206
                     /locus_tag="PLK64_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPV79336.1"
                     /translation="MTTAVVVGSGPNGLAAAIRLAQAGVEVTVVEAHERPGGGTRTSE
                     LTLPGVLHDDCAAFHPTGVASPFFQSLGLERHGLRWLWPEVDLAHPLDDGRAGLAARD
                     LGITDESLGVDAARWRRVFDAPIRGFNDLIDEVFQPVVPLPSHP"
BASE COUNT         1654 a         3907 c         3867 g         1778 t
ORIGIN      
        1 ggaaccagcc tgcgctggcg atgccgagga cctggcccca gggggcgtca ctggcgggag
       61 tgaccgccca gacgagcacc gccatgccga tggtcgcgag ccacgggacc cccactgcga
      121 ccgcgccgct ggcccagacg cgccacgtcg gcgtctgcgg gcgcagggca tcgaccgtcc
      181 cgcgggaggc gtggacggtg gagcggacgc ggtcgaggag gctcatctgc ccctatcgtc
      241 gcccgttcgc ccccttcgac gccggagggc gcgccgggaa gctcccgatc gcgccctcca
      301 gaggtgccgg tgtggaaccg gtcggctggt tcagcgggtt gcgctggttc agcgggtgag
      361 gccctgcatg atctcgcgca tgagctgagc ggtcgccgac ggggtcttgc cgacccggac
      421 gcccgcggct tccagcgcct cggccttagc ctgcgcggtg cccttcgagc cgctgacgat
      481 cgcgccggcg tggcccatcg tcttgccctc gggcgccgtg aagcccgcga cgtagcccac
      541 cacgggcttg gtgacgtgct cggcgatgta tgccgcggcc cgctcctcgg cgtcgccgcc
      601 gatctcgccg atcatgacga tcgcgtcggt gtcggggtcg tcctggaagg cctgcaggca
      661 gtcgatgtgg gtcgtcccga tgatcgggtc gccaccgatg ccgacggccg tcgagaagcc
      721 gaagtcccgc agctcgaaca tcatctggta ggtcagcgtc cccgacttgg acaccagacc
      781 gatccggccg gcaccggaga tgtcggccgg gatgatgccg gcgttggcct gaccgggagt
      841 gatgaggccg gggcagttcg ggccgatgag cctggtcgta cccgcgtcct gcgcgtactc
      901 gaagaactcg gccgtgtcct tgaccgcaac accttcggtg atgacgatcg cgagcgggat
      961 cttggcgtcg acggcctcaa cgacggccgc cttggtgaag gccgccggaa cgaagatgac
     1021 cgagacgtcg gcgccggtgg cctcgatggc ctcggcgacg gtgccgaaga ccggcacctg
     1081 gaccccgccg gggaagtcga cggtcgtgcc ggccttgcgg gggttgacac cgcccacaac
     1141 ctgggtgccc gaggcgagca tccgggtcgt gtgcttcatg ccttcggagc cggtcatgcc
     1201 ctgaacgatg accttggagt cgcgcgtgag gaagatcgcc atgtcgagtg ttccttgtcc
     1261 tagatcttcg tccgggtcag acggctgcag ccgcgagttc ggcgacgcgg cgagccgcac
     1321 cgtccatggt gtcgacgcgt tcgagcaaag gcagcccggc accgtcgagg atctctcggc
     1381 ccttctcggc gttgttgccg tccagccgca cgacgagcgg cttgggcgac ttgccgtggc
     1441 tggcgaggat ctcgtaggcc ttgacgatcc cgtcggcgac ctggtcgcac gcggtgatgc
     1501 cgccgaagac gttgacgaat accgccttga cctggtggtc ctcgaagatg acgtccaggc
     1561 cgttggccat gacgttggcg tccgcgccac cgccgatgtc caggaagttg gccggccgcg
     1621 ggtggctgcc gtcgcgggcg gtgaactctt cgcccgcgta ggccacaacg tcgagcgtcg
     1681 acatcaccag gccggcaccg ttgccaatga tgccgatgtt gccgtcgagc ttgacgtagt
     1741 tgagcgactt ctcgatggcg aggacctcga gcgggtcggt caccgagtgg tcgatgagga
     1801 cctcgtgctc ggggtgacgg aaccgggcgt tgtcgtcgag ggtgaccttg ccgtcgaggg
     1861 cgacgatctg gccgtcctcc atcttcacca gcgggttgac ctcgacgagc gtggcgtcct
     1921 caccggcata cacatcgccg agggtgacca gcacgttgac gagtgcgggc acgtcggcct
     1981 cgtcgaagcc ggccgcagcg atgatctcgc gcgctttggc ctcgtcgagc ccgtcgatcg
     2041 cgtcgaccgc gatccgggcc agcttctcgg ggtgctcgac ggcgagggtc tcgatgtcca
     2101 tgccgccctc cttgctgcac atcgccaggt aggtgcggtt ggtccggtcc agcagcagcc
     2161 cgaagtagta ctcctcggcg atcttggcgc cttcggcgat catcaccttc ttgacggtgt
     2221 ggcccttgat gtccatgccg aggatctgcg cggcgagggc ctgcgcctcc tcaggtgact
     2281 tggcgacctt gacgccaccg gccttgccgc ggccaccggt cttgacctgg gccttgacca
     2341 cgacgacccc gccgctcttg gcgccgaccc gctgcgccgc cgcgaaggcc tcctccgggg
     2401 tcaccgccac ttcgccgccc agcacgggaa ccccgtgcgc ttcgaacatg tcacgtgcct
     2461 ggtattcgta cagatccacg ggcgtccgtc ctcgtcgtcg aaggggtgac cagcgcagcg
     2521 accgtgggcc tgggccgctc gggcagcatt cggactctag tgcacggatt cgggcccttc
     2581 gcccgtcggg aggccagcca tgaccaatgt cacggccggt gtccgcggag tggttgcctc
     2641 gaagttgcgc cgtggttgcg ccgccgctca ggagatcgtc acgccttggc cgactgggac
     2701 aaggagcacc cacagcgggc cggcggccaa ggtgacctcg gcgccggacg ccccggtgag
     2761 tcgggtcgga ctcgcgttcg ttgcccgctc ccaggtcgcg tcatagaccg cgccgccgcg
     2821 cagcaccttc gcctcgcccg tcccgaggag ttcgagcacg ggggtggcgg agccgttgac
     2881 gtcccgattg ttcgaggggt gggttcggat catctggacg aggatgctcg cggcgctgac
     2941 cggtgtgccg gctgcggtca cttccggccg cccgtcggtg gtcaccaggt agcggccgag
     3001 ggtcgcgtcc caggtgaagg ccacccgcgc cagtgggtat gccgttgcga gactcgtcgc
     3061 cggtcggccg ccgggggcgc ccggcccggt tcggaagccg atgtcgcctg ccggggcgct
     3121 gccaccggct cgggccacga gggcagccgg tgtcccgatg acgttgtagg gggccttgcg
     3181 gtcgctggcc cgccggtagc cagccccgcc ggagtccatc gagacgttga cctgcggccc
     3241 tgtgacgatt tccgaggtgg tgattggcga cgcgcccgag aaggcgaagg ccacccggcc
     3301 gtagttgccc agcagaacgg cgtcggactc acgggcgctc cggaccggac caacctcggg
     3361 tgggagggtc gaggcataga tggcgagcaa ccgggtgagg ccggcctcca ccggctcgac
     3421 gtagacgatg tcggcggcgt ccacgccgat ccggggcctt gccggcgacg tgttgtcgat
     3481 cttgacggcg agcaccggtc ccgggccgag caacggcgtg cccaccgcga ccgccgggag
     3541 ggtgttggtc ggcgccgcgg cgcccgttga gatcgacccg gcggtggtcg tggtcgggtt
     3601 tgacggggcc gaggccgatg gggtgcttga gcaggcaacg agcacggcgg cgctcaggcc
     3661 gatggcaaac gcgggccgga tcaccaatgc agactacggg gctggagaac ttatgggctg
     3721 gagcgaatct gccctagacg ttgttgtggg gccacggtag ccgtggcccc acaaccaacg
     3781 tcgtgaagca aaggcactga acaagtcagt gagcaccgtt gccggcgcga gggccacagc
     3841 cgggatgcgg gagtaccggg ggcccttcca cttcgttctc gactcgacgg gccggcgcct
     3901 gggggggagg tgtcgactac gtctgggaac catcatggtc cctttgcccg gcccccgcct
     3961 gccagatcag ggaattctta ctatcttttt actctcgaag gccttgaggt acaccgaggt
     4021 ttagtgcccg tggacgttgg cgcggaccca gtcgacgatc tcgtgggtcg cggcgcccgg
     4081 ggtgaagact ttggcgactc cgagcttctg cagcgccagc aggtcgtcgt cggggatgat
     4141 cccgcctccg aagaccacga tgtcggccgc gtcgcggacc ttgagcaact cgaccacctt
     4201 ggcgaacagg gtcatgtggg cgcccgacag gacggacagg ccgacggcgt cggcgtcctc
     4261 ctgaatggcc gcttcgacga tctgctcggg cgtctggtgg aggccggtgt agacaacttc
     4321 catcccggcg tcgcgcaggg cgcgtgcaac gaccttggcc ccccggtcat gaccgtccaa
     4381 cccgggcttg gcgacgacaa cgcgcagcgg agagtcactc atgcgtcgca ctctagcgaa
     4441 gccccaggca cgtggagcgg gaccagtggc ccaggccacc gcggaggatc catcgccgcc
     4501 agcccagccg ccagcccggc cgccgaccca cccgaccatt ggacagcgag ttctcgtgat
     4561 cctcatcaca ggaggtgcgt cggccccgcg cacactgcgt tcatgggcgc ccgacccgtg
     4621 gacccggctt ggagataacg aatacatcac gctaacctcc acaaggccag ttccagctgc
     4681 gggaagagac caacgacgtg accgacgcca agtaccgtgg gcgccaccgc tctcggcgag
     4741 gtgccgggct cagccctcgc atcgtcggga ccgggctcgt gctcccgaca acggccaccc
     4801 tcgcggtggt gtcagtgacc ggcggagccc cgatggccct caccagcacc actctggccg
     4861 cgggtgccag caccgtggac gatgccacga ccgaccgtgc cgagctcacc caggacaagt
     4921 cgaccgacag caccctgttg gcgcgcggcg tgcaggaccg ggtcgcccgc gacagcgagc
     4981 gcgtgcgggt cgacacggtc gcccaggaga acgccaagcg ggcggcaacc gaagcgtcgc
     5041 tggccgccaa cgacgtcccc gtccaggtgt ccctcgtcgg cgcccctggc tccaagctcg
     5101 ctgcttccgc ggccagcctc aagggtacga acctcggttc caaggcctgg gtcaacccgc
     5161 tcgggggcaa ctacagcctc agctcgaact acggctggcg ttggggtcgc ctgcactccg
     5221 gtcaggacat ggcctgcccg acgggcacgc cggtgcgttc gatctccagc ggcgtcgtca
     5281 ccttctccgg ctgggcgggg aacctcggct acaaggtcga gatcctccac tgggacggca
     5341 ccgtctcctg gtacggccac aacagcaagc ttcgggtcag cgagggtcag cgagtcgagc
     5401 cgggtcagct catcgccctg gccggcagca ccggtaactc gaccgggccg cacgtgcacc
     5461 tggagatcca ccccaacggg ggcaacgccg tcccgccgaa gcggtggctg gctgctcgcg
     5521 gcgtcaacat ctgacgcttc gtgctccgca ggtgagcgat tccggcgtcc tgacggtcgg
     5581 ctccgtcctg acgccggtcg ccactcgcct cagagcttct cgaccggcgc gtaacgcagc
     5641 agcagccgct tgacccccac tgacgccccg aagtcgacgt gggcgagcgt gcgctccccc
     5701 tccccctcca ggcgcagcac cgttccgagc ccgaacgagt cgtggctcac ccgatcaccc
     5761 gcgacaagca cgggcactgc ccggttaccc ggagagcgca cccctggccg agctgccagc
     5821 gtcgccccag ccggctcgct ggaccggcgg ccaaagcgcg ccgaatccga cgccgcagct
     5881 tcgcgctccc acgccaccaa ctcctcgggg atctcgtcga ggaaccgtga cccagggaag
     5941 aactgcggct gaccccaggc cgagcgaacc gcagcccgcg acaggtgcag tcgctcgcgt
     6001 gcgcgggtga tgccgacata agccagccgc cgctcctcct caagctcctt ggggtccgcg
     6061 agcgagcgca gatgcgggaa ggtgccgtcc tcaagaccgg taacgaagac gaccgggaac
     6121 tccaggccct tggcggtgtg cagggtcatc agcgtgacca ctcccccggc atcggcctcc
     6181 gggtcgggga tctcgtcggc gtcagcgacc agcgacacct gctcaaggaa gtcttcgagg
     6241 ctggccggct caccggtctc ggcccgcccc tcgtcgaatt cctgtgcgac agcaaccaac
     6301 tcggcgaggt tctcgacgcg tgtctcgtct tgagggtcgg ggctgttgcg cagctcacgc
     6361 agatagccgc tctgctccag caccgcttcg agcagaccag ccactcccga gccgccctcg
     6421 aagacggtcc gcaggtcttc caacagtcgg gtgaacgcct cgatcgcggc gaccgatcgg
     6481 gtggcgatgc cgggtgcatc gacggcccgc ccgagcgcgg caacgaacgg gatccgctcg
     6541 cggtcggcca actgggccac acaggcctcg gcccgctcgc cgatgccccg ccgcggggtg
     6601 ttgaggatcc gtcgcaggtt gaccgcatcg gtcgggttgg agatcacccg cagataggcc
     6661 aaggcgtcct tgacctcctt gcgctcgtag aaccgggtgc cgccaaccac cttgtaaggc
     6721 aacccgaccc gaacgaacac ttcctcgatc gcccggctct gagcattggt ccgatagaac
     6781 acagcgacgt ccttgggctt caccccatgc tcgtcgccga gccgatcgat ggtccgcgcg
     6841 atgaacgccg cctcgtcatg ctcgttgtcg ccgacgtagc cgatgatccg agccccgtcg
     6901 cccgagtcgg tccagaggtt cttgggccgc cggtccgggt tgcgggcgat gaccgagttg
     6961 gccgcccgca ggatcgtctg ggtcgagcgg tagttctgct ccagcaggat ggtccgcgcg
     7021 ttggggtagt cctgctcgaa ctccacgatg ttgcggatcg tcgccccacg gaaggcatag
     7081 atcgactggt ctgcgtcacc gacgacgacg agctcagcgg gcgggatcgc gtatgccgcc
     7141 cgctcaccac ccgcgcccga cccaccgctc gcgcccgaac caccttctcc tgcagcgtcg
     7201 ctcgtgaccc cgaccagctc acggacgagt tggtactgcg ccgtattggt gtcctgatat
     7261 tcatcgacca gcacatgccg aaaccgccgc cgatagtgct cagcgacatc ggggaacgac
     7321 tgcagcagat gcaccgtcgt catgatgagg tcgtcgaagt ccagcgcgtt ggcctggcgc
     7381 agccgtcgtt gataagcgac ataagcctgg gcgatggtgc gctcgtggtg ggtgccctcg
     7441 tcgccgctca cctgacgggt gtaggtctcc tcgtcgatga gctcgttctt gaggttgctc
     7501 acctgatggc tgaaagaccg cgggttgtag cgcttcgggt ccagatccat gtcccgcagc
     7561 accatcgcca tgagccgctg gctgtcggcc gcgtcataga tggagaagct cgacttgagc
     7621 ccaaacttct cgatctcgcg ccgcaggatc cgcacgcacg ccgagtggaa ggtcatcacc
     7681 cacatcgctc gcgagcgcgc gccgacgaga ctttcgaccc gctcgcgcat ctcggcggcg
     7741 gccttgttgg tgaaggtgat cgcgaggacc tgcccgggct gcaccccccg ctcggcgagc
     7801 aggtaggcga tccgatgggt cagcactcgc gtcttgcccg acccggcgcc ggcgatgatg
     7861 agcagcgggc tgccttcgtg aacgaccgcc gcccgctgtt gcggattgag cccgacgagg
     7921 aaccgttccg ggtcgcccgc cccgcgccct ccggtatgcc gcccgcccgc gtcgggcgcg
     7981 gcatacccgc tgccctcctg ggcccacagc ggcagtcccg cctcatcgac atcgtcctgg
     8041 cgccgggccg gcgtggcgct gggccggaca tccgcgagcg atggcgcgtg cgggtgcgga
     8101 cgtgcgggca cgctcggctc gggctccccg aaaccggaga ggcccaactc atcaaaaagg
     8161 gtgcccatgt cggcgtccac cttatgcgct tggtcggaca ccccgagccg ccacgcactc
     8221 gtcgtaggct cggcgccatg accgacgccc gagcccttcg cgtgacccag cacggcggac
     8281 ccgaggtcct cgccgtccag accgtcgacg tcccggagcc ggggccgggg caggtccggg
     8341 tccgcgtgga ggccgccggg atcaacttca tcgacgtcta ccagcgccag ggggtctatc
     8401 ccgggccgac cccgttcacc cccggcatgg aaggcgccgg gatcgtcgag tcggtcggcg
     8461 ccgacgtcga cctcctcgtg gggtctcggg tggcgtgggc gatggcgcgg ggggcggcgg
     8521 ccgagctcgc ggtgctgccg gcggcgtccg cggtgcccgt cccgaacgac atcaccgctg
     8581 agcaggcggc agctgcgatg ttgcagggca tgaccgcgca ctacctcacc accagcacgt
     8641 atgccgtcca gcccggtgac ttcgtcctcg tccacgccgc agcgggcggg gtcgggcagt
     8701 ggctggtcca ggtctgttcg ctcgcggggg caactgtcat cgccaccgca gggtccgagg
     8761 caaagttggc gatcgcccgg gagttgggcg cggcctacac gctggactac acggcatacg
     8821 cgacgccggc ggagttggcg gcagcggtgc gcgaggcgac cggcggcgtg ggtgtggcgg
     8881 tggcctatga cggggtcggc aaggcgacct tcgatgcgtc gctgggctcg ctgcgtccgc
     8941 gcgggatgct ggcgctcttc ggcggggcga gcgggcaggt gccgccgttc gacctgcagc
     9001 ggctcaacgc ggccggctcg gtgttcgtca ctcgtccgac gctcgcgcac tacgtcgccg
     9061 accggcacga gttgctgtgg cgggcgggcg aggtcttcca gacgctggcg tcggggcggg
     9121 tccggatgga catcggcggg gtctatgcgt tggaggatgc cgccgccgcc tacgaggcct
     9181 tggagggccg ggccacgacc ggcaaactga tcttccagat ctgagttgag ccaccccagc
     9241 cccaccccca ccccccttgc cgggtcgagg tattccacca tcgtgagaca acggccgagt
     9301 acctctatcc ggcccaggaa accgcaagga agcggcccat gagcgacaac gtccgggtga
     9361 cccgcgagga ctcgaccacg acgatcaccc tggcccggcc ggagaagcgc aatgcgctct
     9421 cggcggcgat gatgaccgac ctcacggccg cgctggccga ggccggcgcc tccgacacgc
     9481 tcgccgtggt gatcgccgca gacgggccgg tgttctcggc gggccataac ttcggcgacc
     9541 tggcgagagc cgacctgcgg gcggcccgcg agctgttcct gctgtgcacg acgatgatgg
     9601 acacggtcca gggcatcccg cagccggtca tcgccaaggt gcacgcccta gcgaccgcag
     9661 ccgggtgcca actcgtggcg tcctgcgacc tggcgattgc tgccgacacc gctgggttcg
     9721 cgctgcctgg tggcaagggc gggttgttct gtcacacccc gctcgtcgcg gtctcgcgca
     9781 acgtcgggcg caagcgggct ctggaaatgg cactcaccgg cgacgtcatc gacgcggcga
     9841 ccgccgccga ttgggggctg atcaaccgcg cagtgcccgc cgatgacctc gacgccgctg
     9901 tgtccgacct ggtcgcacgg gtgacccgcg ggagcgccat gggccgctcg atcggcaagc
     9961 gcacgttcta tgcccaggtc gggttggagc agcggtcggc ctacgacctg gccgtcgagg
    10021 tcatggccgc ggcggcgttg acggatgacg cacaggaggg gatcgcggcc ttcctcgcga
    10081 agcggcacgc ggcatacacc tcgctcccct gacccctcgg cgcctgctgt cgcagactgc
    10141 tagcatagtg cgacatgagg accctctacc tgcgcaacgt gcccgatgag gtgggagacg
    10201 cgctggaaga gctggcttcc cgtgcctcca tgtccgtcag cgcgtatgcc gtgcgcgagc
    10261 tctccgccgc ggtcgccttc cggtccaacg ccgacctgct gtggacccag ccgcaggtcg
    10321 aggtcgacat cgacgacgtc gtcgcggcgg tccgttcggg gcgcgatcgt tgatcgtcgt
    10381 cgacacgtcg gtcgttattg cggcgctcct cggccaagac cccgcccggc aggccctcgt
    10441 cggccgtcga ttggtggcac cgcatctcat tgatgccgag gtggcacatg ccctgcgcgg
    10501 cctcgtattg ggcgtgaagg tcactgcgga cgatggcgag cggctgctgt cggtgtggca
    10561 gcacctcgcc gtagaccgac tgcccatgac tgggctgctc ccccgagcgt gggcacttcg
    10621 tgacgcgctc acggcatacg acgcgatgtt tgtggccgcg gccgaagccc tgcacgtccc
    10681 cctcgtgacc tgcgaccggc ggctggctca cgcgggtgcc gcacggtgca ccgtcgaaat
    10741 cgtgcccggg gcgagctcga tgccgacatg actacggccg tggtggtcgg cagcggaccc
    10801 aacggactcg cggcggcaat ccggctggcc caggccggtg tggaagtcac cgtcgtcgag
    10861 gcccacgagc ggcccggggg cggcacccgg accagcgaac taaccctgcc gggtgtgctg
    10921 cacgacgact gtgccgcgtt ccacccgacc ggcgtcgcct cgccgttctt ccagtctctg
    10981 ggtctggagc gacacgggct gcgctggctc tggcccgagg tcgacctcgc ccacccgctg
    11041 gacgacggcc gggccgggct ggccgcccgc gacctgggca tcaccgacga gtccctgggt
    11101 gtggatgcgg cccgctggcg gcgcgtgttc gatgccccaa tccgggggtt caacgacctc
    11161 atcgatgagg tcttccaacc ggtcgtcccc ctgccgtccc atccgg
//
LOCUS       DAOMZY010000077        11182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5448_length_11182_cov_5.730296, whole genome shotgun sequence.
ACCESSION   DAOMZY010000077 DAOMZY010000000
VERSION     DAOMZY010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 11182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11182
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5448_length_11182_cov_5.730296"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2252
                     /locus_tag="PLK64_06045"
     CDS             <1..2252
                     /locus_tag="PLK64_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79337.1"
                     /translation="LAGTVTPIVAGLQGYAAALTEARRRVTLLRQEWDWGARRHDEAR
                     HDAELAGSDPVAVALGAAAGQRLAARQEADRAWARLQEDLRGRHTAALGPLTAAAERA
                     TATVRGFLAALPGDDAEAVRGGLLAQLPLTAGALRLADARALIGDLVSRVGRPVQEWG
                     APEADLIATLGDRVRDPFVAQALMEAIPPEHLGRLVERLLANVASVPGADVTWKHRFD
                     PALDVLGSAFLVLASQDPWAGMDEASVARSDSWRRRWLGDLVDTGPGRIGDPLADPPF
                     RGFTAQAVLLDHGRRSVPSVGAGTAYAASVGVALVEADRISDDYPPQVQLPWAARSAA
                     DGVDPIASLLRSIRGEPDAASALLLARLGDGQPVVRYLAGERLVRGAGWPPPAANDAL
                     AALLAETATGTDERSVTIAGAALDGFGAAAEHPERPGNGDAAAGALALDGQMQGLRFV
                     MADLLARHPDAVWASVNDPINALAWPIDAADGHPWAVLGADAWTLRLLNRSRLAAVLG
                     ELGKDRLHPGTAASGPPTAPALAHLLNALVAAQGVGLAQALASGNAQDQDAQIVQLGQ
                     VTGFVIEAARQSVMTIQAGADAEIAARREFVDSIVAMVSLPASLKASLPAALTAPLLG
                     LVKAAIKRIGYSDGSVDNAATADAQTAAARARLDDDLRHLGWDLVSAAGWWDVADDPV
                     AWIEAHPGVSFCGEDGRPVPVEEMSAAQRERFRAWTREVPAYTTVPSAIVEQIEEGAR
                     AVRTAIAGR"
     gene            complement(2227..2655)
                     /locus_tag="PLK64_06050"
     CDS             complement(2227..2655)
                     /locus_tag="PLK64_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79338.1"
                     /translation="MSRVRIYLPLTAQDVESLAAGTSLTRSTGYAVTVRLASVTPGAD
                     PEELEYAAFLDAAAAAGRARGADRSARRVVAAADVEAESVTEPALPDGRTPAEVALAA
                     PVTARAIVSFHVDELAGGTDDGDLLWFDASELISVQQLLS"
     assembly_gap    2800..2899
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2900..3955)
                     /locus_tag="PLK64_06055"
     CDS             complement(<2900..3955)
                     /locus_tag="PLK64_06055"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPV79339.1"
                     /translation="MTRRGLVLGGGGVLGAAWTVGALTALEEQLGIDARDFDEFVGTS
                     AGSVLAALLACGVDVAALREHQLGAEITAGPLSGFHYDHDTAAGGSRPPRPRAALGAP
                     AVIRRGVSGLRRLPPTTVVSSFLPVGTGRLDRVGALIKHVAPAGWAPRAGLTVVALDY
                     DASERIAFGRNGAPEVDLADAVMASCAIPAWYEPVSIHGRRYVDGGAWSSTNIDLMVG
                     LDLDEVYVLAPAVTFDPDTPTRAMTRLERRWRTRVTRRALRELAEVHRDGAQVTLLGP
                     SSLDLEVMGHNIMQAQRRMEVLQTSLQTSRVSLGDPHELGIEQGHVPHEPESADAVTA
                     YTAVDQTESDAASDPAPD"
     gene            complement(3952..5379)
                     /locus_tag="PLK64_06060"
     CDS             complement(3952..5379)
                     /locus_tag="PLK64_06060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HPV79340.1"
                     /translation="MADRLTSLDTSFLYMEDRATVMHVGEVMVFDPPGGRLDHERLMG
                     LFAQRIAQLPRYRQKVCWVPGNLANPVWIDDETFDLSYHVRRAALPKPGGDAQLEEFV
                     ARVQPRPLDRGRPLWEAYIVEGLEHGRFAIVTKTHHALVDGAQPLDIGQVLVSDTPGW
                     EKPLPDLWRARHSPSDLSLLTSAVVDSVKGPRALLRSVRGGSQELVDLGGRVVSGVGS
                     LVSTVARGAARPAPTSPLNAHVGQARRFVMVGTDLAAYRAVRRRLSKGPHADDVTVND
                     IVLACIAGALRTWLLTRGSPVHSTTVVRALVPVSVEDESGQGLHQLAACLIDLPVGEA
                     AASMRLHQVAFLMRQQIAGGHAVGADRLVGLAGFAPPTLHAMGARLGSDMSKRLFNLI
                     VTNVPGPQFPLYAGDAHLLATYPVIPLAKGQALAIGLTSYDGGVYYGLNADRDAMPDI
                     EVLGQGIADALAELANGADGSASDA"
     gene            complement(5424..>6420)
                     /locus_tag="PLK64_06065"
     CDS             complement(5424..>6420)
                     /locus_tag="PLK64_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79341.1"
                     /translation="AAADIDPIPVRDTLGHVVAVWGPTGSPGRTTVAVNLAVELALLG
                     REVLLIDADTYGGCVAQTLALLDEAPGIATAARAAEQGTLDLAALARLALQVRPGLRV
                     LTGISKAERWPEIRGAALERVLQVCRALVDDVVVDVGFCLEDDEELSYDTQAPRRNQA
                     TLTTLQLADAAVVVGGCDPVSLQRLVRGLQELGTVRAPEPIVVVNRVRSGPVGSQPER
                     LVADALERFAGVVQVHTIPDDPAAFDAAMLAGRALAEIVPAAPARRAFLDLVGEVVRR
                     APLPTTSEFEDSAGRSGVSTGPGATRAQGLRRRGSDASGRRGRRHTSRGWGRSRG"
     assembly_gap    6421..6520
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6521..6990)
                     /locus_tag="PLK64_06070"
     CDS             complement(<6521..6990)
                     /locus_tag="PLK64_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79342.1"
                     /translation="MTPGAAGLPSVLTAVSARWDGPVVAAVEASRQLTIARRCADLAD
                     LLAAAASGLGRVALVSGQLRGLTLSTVATLRDGGVEVVGLADPDDDQGELRLWQLGVR
                     SVVSATAAPHDLVTALLDAAGAGHHAGAGADRSEVGGGWVPESLGEAAGADPILA"
     gene            complement(6987..7673)
                     /locus_tag="PLK64_06075"
     CDS             complement(6987..7673)
                     /locus_tag="PLK64_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79343.1"
                     /translation="MSTHGGQTGPGVSVRATRLQRPSWRDSRLIVGIVLVLLATALGA
                     KAVASADDRVPRYAAATTLKPGDRLDAGTIVRVDVLLDDAVAAYLSAQTPPPADSFVL
                     REVRAGELVPVSAVGSRSDVSLQPVTVRVDGDSVAGLISGSVVDVWVSPRDPESAQER
                     YLGAALGLHAVAVAWIPTDQGSFGVSTSSSAVTLLVPAADVQKLIAAQDARSRLTLVP
                     VPGSLHGRGS"
     assembly_gap    7719..7818
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7840..8088
                     /locus_tag="PLK64_06080"
     CDS             7840..8088
                     /locus_tag="PLK64_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV79344.1"
                     /translation="MPPRFLQLADVAEILNISSAQAYALVRSGDLPAIKVGGRGQWRV
                     ESEQLEGYIQRMYAATREFVVAHPLGGGTGEDDDADEG"
     gene            complement(8103..8651)
                     /locus_tag="PLK64_06085"
     CDS             complement(8103..8651)
                     /locus_tag="PLK64_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79345.1"
                     /translation="MRWERLFDDLEAQAAAEADLELAAEVADRTRRERAVLGLHERLA
                     GMRRVGAVSAEVAGLGAVRAVVADVGADWVLLAPTPERSVLVPFGALRSVVGLAWRIA
                     PVGAVAKGFTVGAALRAISRDRAVVTIVDRDGQRRSGTIDAVGQDAVDLAEHPIDLPR
                     RPEHVGDVRTIPFAWIGAVLRV"
     gene            8792..9616
                     /locus_tag="PLK64_06090"
     CDS             8792..9616
                     /locus_tag="PLK64_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPV79346.1"
                     /translation="MSHSPSLPWRAFGVALAGAVGCSLVAVVFVRIGVSSWALASRPG
                     TGRPEDALLALIALLGALLTAWLAMATVLAALAEVPGAVGSACARLSSRITPAVVRRL
                     VTLAVGSALVAATAPATASAVTAATAARHTVVATLDQPPDPTFRPLAEPAPGPAVAVS
                     TPDPGFVPLAPSAPVAAATSIDTLERAPRQLPEAGEMHVVRRGDTLWDIAAARLGPGA
                     TAGDIAAAWPRWYAANRRLIGPDPSHIVPGQRLVPPPEGTLAGSPAAVLTSPGASR"
     gene            9613..10125
                     /locus_tag="PLK64_06095"
     CDS             9613..10125
                     /locus_tag="PLK64_06095"
                     /inference="COORDINATES: protein motif:HMM:NF039460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3235 family protein"
                     /protein_id="HPV79347.1"
                     /translation="MSSALASSPRRVHVVPLPESRPPVVPPDEVFARKESEFVQDSLA
                     LDFLARDELFAHQLSARAELPDPAPLVAALAQAFVEVMAGLRPPPQVSRWTSPEVYAA
                     LCRRAAVATRRGPASRRPAVVRRVRVQEPADGVVEACAVVIQQDRVRALALRLTGLDR
                     RWIVTELSVG"
     gene            complement(10167..>11182)
                     /locus_tag="PLK64_06100"
     CDS             complement(10167..>11182)
                     /locus_tag="PLK64_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPV79348.1"
                     /translation="SIAGAQSQVEAQNYEIRKNVLKYDDVLNRQRQVIYAERRQVLEG
                     ADMEEQIRHFINDVVAAYVNSATAEGYSDTWDLDQLWTALGTLFPVSITVEDVATRAG
                     GRSAVSRDVLLEELTSDAHHAYDQRQELLGEENMREIERRVVMSVLDRKWREHLYEMD
                     YLQEGIGLRAMAQRDPLVEYQREGFQLFEAMMDGIKEESVGFLFNAPVQIEQTPAPAP
                     APTPIRVSDMVRNLAASGANPQDASDEDDDLDADDDEGMPSAPADPLAGTAFSRSTPG
                     VSVKGLEATPQATRLHYSAPGETGSVEHREVDAAGGAVPDSGGYRRPTPRRSSKKARR
                     RGK"
BASE COUNT         1586 a         3943 c         3851 g         1502 t
ORIGIN      
        1 agctcgccgg caccgtgact cccatcgtgg ccggactgca ggggtatgcc gcggccctca
       61 ccgaggcccg gcgtcgagtg acgttgttgc gccaggagtg ggactggggc gcccgccgac
      121 atgacgaggc gcggcacgac gccgagctcg cggggagcga tccggtcgcc gtcgcactcg
      181 gtgcggctgc cggccaacgt ttggccgccc ggcaggaggc cgaccgcgcg tgggcgaggc
      241 tgcaggagga cctgcgcgga cggcataccg ccgcgctcgg gccgttgacc gcggcggcgg
      301 agcgggcgac ggcgacagtc cggggtttcc tggcggccct gccgggcgac gacgccgagg
      361 cggtccgagg tggcctcctc gcgcagttgc cgttgaccgc gggcgcgctg cggctggccg
      421 atgcccgggc cctgatcggc gacctcgtgt cccgcgtggg acgccctgtg caggagtggg
      481 gggcgcccga agccgacctc atcgccacgt tgggcgaccg ggtccgcgac ccgttcgtgg
      541 cgcaggcgct gatggaggcg atcccacccg agcaccttgg gcggctcgtc gagcggctgc
      601 tcgccaatgt cgcctccgtt cccggcgcag acgtgacgtg gaaacatcgg ttcgatccgg
      661 cactggatgt cctcgggagc gccttcctcg tcctggcgag tcaggacccc tgggccggga
      721 tggacgaagc gagcgtcgcc cggtcggatt cctggcgtcg tcgatggctg ggcgacctgg
      781 tcgacaccgg gccgggccgc atcggcgacc ccctcgccga cccgcccttc cgcgggttca
      841 cggcgcaggc cgtgctactt gaccatgggc ggcgcagcgt gcccagcgtc ggcgcgggga
      901 cggcatacgc ggcctcggtg ggggtcgcgc tcgtggaggc cgaccggatc agcgatgact
      961 acccgcctca agtccagcta ccgtgggcgg cccggagcgc ggccgacggc gtagatccca
     1021 tcgccagcct gctgcggtcg atccgtggcg agccggacgc ggcatccgcg ctgctgctcg
     1081 cacggttggg cgacgggcag cccgtcgtgc ggtacctcgc cggcgaacgg ctggtgcgag
     1141 gtgcgggttg gccgccgcct gcggcgaacg acgcgctcgc cgccctgctg gccgagactg
     1201 ccaccggcac tgacgagcgt tcggtgacga tcgccggcgc ggcactggac ggcttcggtg
     1261 cagcagccga acaccccgaa agaccgggca acggtgacgc tgcggcgggg gcactggctc
     1321 tggacggcca gatgcaaggg ctgcgcttcg tcatggccga cctgttggcc cggcaccccg
     1381 atgccgtctg ggcgagcgtt aacgatccga tcaacgcgtt ggcctggccc atagacgctg
     1441 ccgacgggca cccctgggcg gtcctcggcg ccgacgcgtg gacgctccgg ctgctcaaca
     1501 ggtcacggct ggccgccgtc ctcggcgagc tcggcaagga ccgactgcac cccggcacgg
     1561 cggcgagcgg tccacccaca gcgccggcgc tggcccacct cctcaacgct ctcgtcgccg
     1621 cgcagggggt ggggctcgcc caggccctgg cctccggtaa cgcgcaggac caggacgctc
     1681 agatcgtcca gctcggccag gtgaccgggt tcgtcatcga ggcggcccgt cagagtgtga
     1741 tgaccattca ggccggggcc gatgccgaga tcgctgcgcg gcgggagttc gtcgattcga
     1801 tcgtggccat ggtgagcctt ccggccagcc tcaaagcctc gcttcccgcc gcgctgaccg
     1861 cacccttgct cggcctggtg aaggctgcga tcaaacggat cggctacagc gacggctccg
     1921 tcgacaacgc tgcgaccgcc gacgcccaga cggcggcggc gcgggctcgc ctcgacgacg
     1981 atctgcgcca cctgggttgg gacctcgtca gcgcagcagg ctggtgggat gtcgccgacg
     2041 acccggtggc ctggatcgag gctcatcccg gggtgtcctt ctgcggcgag gacggacggc
     2101 cagtgccggt ggaggagatg tccgcggccc agcgcgagcg tttccgagcc tggacgcggg
     2161 aggtcccggc ctacaccacc gtgccttcgg cgatcgtcga gcagatcgag gagggagcgc
     2221 gggcggtcag gacagcaatt gctggacgct gatgagctcg ctggcgtcga accacaacag
     2281 gtcaccgtca tcggttccgc cggcaagctc gtccacgtgg aaggagacga tggcccgggc
     2341 cgtcaccggg gcggccaggg cgacctccgc cggagtgcgg ccgtcgggca gcgcgggttc
     2401 ggtcaccgac tccgcctcga cgtcggcggc tgccacgacg cgtcgggcgc tgcggtcggc
     2461 tccgcgcgca cgcccggccg cggcggcggc atcgaggaac gcggcatact ccagctcctc
     2521 ggggtcggca ccaggggtga cgctggccag gcggacggtg acggcatacc ccgtggatcg
     2581 ggtgagtgac gtcccggccg ccagcgactc gacgtcctgg gcggtgaggg gtaggtagat
     2641 ccgaacgcga ctcacgggcg gtcctcctgt gtcagaacgg acgggtatgc cgcggggtcc
     2701 ggcacggcgt caggtgccgg gtcgggagcg gcgtctgcca cggggtcggg cgccgggtcg
     2761 gaggcggcat ccgattcggt ctgatcgacc gcggtgtagn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnng tcgggcgccg ggtcggaggc ggcatccgat tcggtctgat
     2941 cgaccgcggt gtaggcggtc accgcgtcgg cgctctccgg ctcgtgcggg acgtgcccct
     3001 gctcgatccc caactcgtgc gggtccccca gcgagacgcg cgaggtctgc agcgaggtct
     3061 gcaggacctc catccgccgt tgggcctgca tgatgttgtg gcccatgacc tccaggtcga
     3121 ggctgctggg ccccaacagg gtcacctgag cgccgtcgcg gtgcacctcg gcgagttcgc
     3181 gcaacgcccg ccgggtcacc cgggtgcgcc agcgccgctc gagccgagtc atcgcacggg
     3241 tgggagtgtc ggggtcgaag gtcaccgcgg gggcaaggac atagacctcg tccaggtcca
     3301 ggccgaccat gaggtcgatg ttggtcgacg accacgcgcc accgtcgacg tagcgtcggc
     3361 cgtggatgct gacgggctcg taccaggccg ggatggcgca cgatgccatc accgcgtcag
     3421 caaggtcgac ctccggggca ccgttgcggc cgaacgcgat tcgttctgag gcgtcgtagt
     3481 cgagcgcgac gaccgtcagc cctgcccggg gcgcccatcc ggcgggcgcc acgtgcttaa
     3541 tcaaggcgcc gactcggtcg agtcggcccg taccgacggg gaggaacgag gagacgacgg
     3601 tcgtcggagg aaggcggcgc aagccactga cgccgcggcg gatgaccgcc ggtgccccga
     3661 gcgcagcccg cggccggggc ggccgtgacc ctcccgctgc ggtgtcgtgg tcgtaatgga
     3721 acccagacag cggccccgcc gtgatctcgg ccccgagttg gtgctcgcgc aacgcagcaa
     3781 catcaacgcc gcaggccagc agagcggcca gcaccgaccc ggcagacgtc ccgacgaact
     3841 cgtcgaagtc gcgggcgtcg ataccgagtt gctcttcgag cgcggtgagt gcgccgacgg
     3901 tccaggccgc gccgagcacc cctccgccgc cgagaaccag acccctgcgg gtcatgcgtc
     3961 gctggctgag ccgtcggcgc cgttggcgag ctcggcgagc gcatcggcga tcccctgccc
     4021 caggacctcg atgtcgggca tcgcgtcgcg gtcggcgttg aggccgtaat agaccccgcc
     4081 gtcataggac gtcagcccga tggccagcgc ctgccccttg gcgagcggga tcacggggta
     4141 ggtcgcgagc aagtgggcgt caccggcata cagggggaac tgcggtccgg gcacgttggt
     4201 gacgatgagg ttgaacagcc gcttggacat gtcgctgccc agccgcgcgc ccatggcgtg
     4261 cagcgtcggc ggagcgaaac cggccagccc gacgagccgg tcggcaccga cggcatggcc
     4321 gccggctatc tgctggcgca tgaggaaggc gacctggtgc aggcgcatcg acgcggcggc
     4381 ctcgccgacg ggcaggtcga tcagacaggc ggccagttga tgcaggccct ggcccgactc
     4441 gtcctcgacg ctgaccggaa cgagggcacg gaccaccgtc gtcgagtgga ccggcgaacc
     4501 gcgcgtcaac agccaggtgc gcaacgcccc ggcgatacag gccagcacga tgtcgttgac
     4561 ggtcacgtcg tcggcgtgcg gccccttgga gagccggcga cggaccgccc ggtatgccgc
     4621 gaggtcggtg ccgaccatga cgaaccgacg cgcctgtccg acatgggcat tgaggggtga
     4681 ggtgggagcg ggacgggccg cgccccgcgc cacggtcgac accagcgacc cgacgccgga
     4741 gaccacacgt ccaccgaggt ccaccagctc ctgagaccca ccgcgcaccg agcgcagcaa
     4801 ggctcgcggg cccttgaccg agtcgaccac cgccgaggtc agcaggctga ggtcgctcgg
     4861 gctgtgccgg gcccgccaca ggtcgggcag cggcttctcc cacccgggcg tgtcgctgac
     4921 cagcacctga ccgatgtcca gcggctgagc gccgtcgacg agcgcgtggt gggtcttggt
     4981 gacgatggcg aagcggccgt gttcgagccc ttcgacgatg tacgcctccc agagcgggcg
     5041 tcctcggtcc agcggccggg gttgcacccg ggcgacgaac tcctccaact gggcatcgcc
     5101 accgggcttc ggcagcgcgg cccggcggac gtggtagctg aggtcgaaag tctcatcgtc
     5161 gatccacacc ggattggcga ggttgcccgg cacccagcac accttctgcc ggtaacgggg
     5221 cagttgggcg atccgctgcg cgaacaagcc catcagccgc tcatgatcca gccgcccacc
     5281 cggcgggtcg aagaccatca cctcgccgac gtgcatgacc gtggcccggt cctccatata
     5341 gaggaacgag gtgtccagcg aggtcaagcg gtcagccacc ggaccatcct gtcagatcgc
     5401 tccgaccccc ggccgccgcc gactcacccc cgcgaccgcc cccacccacg cgaggtatgc
     5461 cgccgtcccc ggcgccccga ggcgtctgac ccacggcgcc gaagaccctg ggcgcgcgtg
     5521 gcccccgggc cggtcgagac cccggaccta cccgccgaat cctcgaactc ggacgtggtc
     5581 ggcaggggtg cccgacggac cacctccccg accaaatcca ggaaggcacg cctggccggg
     5641 gctgcaggaa cgatctcggc caaggcccga ccggcgagca tggcggcatc gaaggcggcc
     5701 ggatcgtcag ggatggtgtg cacctgcaca accccggcga aacgttcgag cgcatccgcg
     5761 acgagccgct ccggctgcga cccgaccggt ccggaccgga cgcggttgac gacaacgatg
     5821 ggctcggggg ctcggaccgt gccgagctcc tgcaaccccc ggacgagccg ctgcagggac
     5881 accggatcgc agcctccgac gacgacagcg gcgtcagcca gctgcagcgt cgtgagcgtc
     5941 gcctgattgc ggcgcggcgc ctgagtgtcg tagctgagtt cctcgtcgtc ctccaagcag
     6001 aagccgacgt ccacgacgac atcgtcgacg agcgcgcggc atacctgaag gacccgctcc
     6061 aacgcggccc cgcggatctc gggccagcgt tcggccttgg atatcccggt gaggacccgc
     6121 aaaccgggac ggacctgcaa cgccagccga gcgagggcgg cgaggtcgag ggtgccctgc
     6181 tcggccgctc gggccgcggt cgcgatgcct ggggcttcgt cgagcagcgc gagggtctgc
     6241 gcgacgcacc cgccataggt gtcggcgtcg atcagcaaca cctcgcgccc cagcagagcg
     6301 agctcgacgg cgaggttgac ggcgaccgtg gtgcgccctg gcgagccggt cgggccccag
     6361 accgcaacca cgtggccgag ggtgtcgcgc accgggatcg ggtcgatgtc ggcggcggcg
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gccaggatcg ggtccgcacc
     6541 ggccgcctcg ccgagcgact ctgggaccca cccgccgcca acctccgagc gatcagcacc
     6601 agcgcccgcg tggtggccgg cccccgcggc gtccagcaac gccgtcacca agtcgtgcgg
     6661 ggccgccgtc gcagagacga ccgagcgcac cccgagctgc cacaaccgca gctcaccctg
     6721 atcgtcgtcg gggtcggcga gacccaccac ctcgacgcca ccgtcacgaa gggtggcgac
     6781 ggtcgagagg gtcaggccac gcaactgacc tgacaccaat gccacccggc ccagccctga
     6841 ggcagccgcc gccagcagat cggccaggtc ggcacaccgg cgcgcgatcg tgagctgccg
     6901 agaggcttcg accgcagcca cgaccggccc atcccaccgg gccgagacag ccgtgaggac
     6961 agacggcagc ccggcggccc cgggggtcat gagccccgtc cgtgcagcga gccgggcacc
     7021 ggcacgaggg tgagccgcga tcgggcgtcc tgtgccgcga tgagtttctg gacgtcggcc
     7081 gcgggcacca acaaggtcac ggcggacgag ctcgtcgaca ctccgaacga cccctgatcg
     7141 gtcgggatcc aggccaccgc gacggcgtgc agccccaacg ccgcgccgag gtagcgctcc
     7201 tgggccgatt cggggtcgcg tgggctcacc cacacgtcga cgaccgaccc ggagatcagc
     7261 ccggccacgg agtcaccatc gacccggacg gtgaccggct gtagggacac gtccgaacgc
     7321 gagcccacgg ctgacacggg gacgagctca ccggcacgca cctcacgcag cacgaacgag
     7381 tcggccggag gcggggtctg cgccgacagg tatgccgcca ccgcatcgtc gagcagcacg
     7441 tccacgcgca cgatcgtgcc cgcgtcgagg cgatcaccgg gtttaagggt ggtggccgcg
     7501 gcataccgag ggacgcggtc atcggcgctg gccaccgcct tggccccgag cgcggtcgcc
     7561 agcaggacca ggacgatgcc gacgatcagt cggctgtcgc gccacgacgg gcgctgcaag
     7621 cgcgtcgccc ggacgctcac gcctggcccg gtttgccccc catgtgtgct cacgcgccca
     7681 ttgtggtggt ttgtcggctt cccttccacc gaccgcgann nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnngg acgcgcctat gctgagccca tgccgccgcg cttcctgcag
     7861 ctcgccgacg tcgccgagat cctcaacatc tcatcggccc aggcctatgc gctcgtccgc
     7921 tcgggcgacc tcccggccat caaggtgggc ggccgcgggc agtggcgggt ggagagcgag
     7981 cagctcgagg gctacatcca acgcatgtat gccgcgaccc gcgagttcgt cgtcgcccat
     8041 cccctcggtg gcggcaccgg cgaggacgac gacgccgacg agggctgacc gaagccgtcc
     8101 cgtcagaccc gcaggactgc gccgatccag gcgaacggaa tcgttcgcac atcgcccacg
     8161 tgctcgggcc gacggggcag atcgatgggg tgctcagcca ggtcgacggc atcctgacca
     8221 accgcgtcga tcgtccctga ccgccgctgc ccgtctcgat caacgatggt cacgacggcc
     8281 cgatcccgac tgatcgcgcg cagcgcggct ccgacggtga accccttggc gaccgcaccg
     8341 actggggcga tccgccaggc cagccccacg accgatcgaa gggcgccgaa cggaacgagg
     8401 acggaacgct ccggagtggg cgccagcaag acccagtcgg ccccgacgtc cgccacgact
     8461 gcgcgcaccg cgcccaaccc cgcgacctcc gcagagacgg cgccgacgcg acgcatcccc
     8521 gccaaccgct cgtgcaaccc cagcaccgct cgttcgcgac gggtgcggtc ggcgacctcg
     8581 gcggccaact ccaggtcggc ttcggcagcc gcctgtgcct ccaggtcgtc gaacagccgc
     8641 tcccatcgca tccggtcatc cttccacccg cacccatccg acgcgtctcg acagcacatg
     8701 cctctctgga caccctcgca aactcttgac aatggtctca ttcacaggca aacataggca
     8761 aacgacatca accgtgagat ctgggggacc catgagccat tcaccgtcgc ttccgtggcg
     8821 ggccttcggc gtcgccttgg ccggcgccgt gggctgctcg ctcgtcgctg tcgtcttcgt
     8881 tcggatcggc gtgagcagtt gggcgctggc cagccggcct ggcactggcc ggccggagga
     8941 cgccttgttg gcgctgatcg ccctgctcgg tgccctgctg acggcctggc tggcgatggc
     9001 gacggtgctg gcagccctcg ccgaggtccc gggggcggtc gggtcggcct gtgcgcggtt
     9061 gtccagccgc atcacacctg ctgtcgtccg tcgactcgtc acccttgccg tcggttcagc
     9121 actcgtcgcg gcaaccgcgc cggcgaccgc ctcagccgtc accgcggcca ccgccgcgcg
     9181 gcataccgtg gtcgccaccc tcgaccagcc gccagacccc acgttccggc ccttggccga
     9241 gccggccccg ggccctgcag tggcggtgtc cacgcccgac ccgggcttcg tgcccctggc
     9301 ccccagcgct cctgtcgccg ccgcgacgag catcgacacg ctcgaacgcg ccccacgcca
     9361 gctcccggag gccggcgaga tgcacgtcgt gcgtcgaggc gacaccctgt gggacatcgc
     9421 cgccgcccgc ctcgggccgg gcgcgacagc gggcgacatc gccgccgcct ggccgcggtg
     9481 gtatgccgcc aatcggcggc tcatcgggcc cgacccctcg cacatcgtcc ccggtcaacg
     9541 cctcgtcccc ccacccgagg gcaccctggc cggctcgccc gcggcggtgc tgacctcgcc
     9601 tggagcctcg cgatgagctc agccctcgca tcgagcccgc ggcgggtcca cgtcgtgccg
     9661 ctgccggaga gccggccacc ggtggtgcct ccggacgagg tcttcgcccg caaggagagc
     9721 gaattcgtcc aggacagcct ggcgttggac ttcctggccc gcgatgagct tttcgcccat
     9781 caactctcgg cgcgcgcaga actgcccgac cccgcgcctc tggttgcggc gctggcccag
     9841 gcctttgtcg aggtcatggc cgggttgcgg ccgccgccgc aggtgagtcg gtggacctca
     9901 cccgaggtgt atgccgcgct ctgccgccgg gccgcagtcg ccactcgccg gggcccggcc
     9961 tctcggcggc ctgccgtggt gcgtcgggtg cgggtccaag agcctgctga cggagttgtc
    10021 gaggcgtgtg ccgtcgtcat ccaacaggac cgggtccgcg ccctggccct gcgcctgacc
    10081 ggcctggacc ggcgctggat cgtcaccgag ttgtcggtcg gctgaccgca tcgaaccagg
    10141 ctgacgagtt ggtcgcccgg cctcgactac ttgccgcggc gacgagcctt cttgctcgac
    10201 cggcgcggcg tcggccgacg gtacccaccc gagtcgggca cggcacctcc agccgcatcg
    10261 acctcacggt gctcgaccga tccggtctca cccggcgccg agtagtgcag ccgggtcgcc
    10321 tgcggcgtgg cctccaaacc cttgacgctg acacccggcg tcgagcgcga gaacgccgtg
    10381 ccggccaacg ggtcggccgg ggcggacggc ataccctcgt cgtcgtcggc gtcgaggtcg
    10441 tcgtcctcgt cgctggcgtc ctgcgggttt gcgccggagg cggcgaggtt gcggaccatg
    10501 tcgctgaccc gaatgggcgt cggcgcgggg gccggagccg gggtctgctc gatctggacc
    10561 ggggcgttga agaggaagcc gaccgactcc tccttgatcc cgtccatcat cgcttcgaag
    10621 agctggaagc cttcgcgctg gtattccacg agcgggtcgc gctgagccat ggcccgcagc
    10681 ccgatgccct cttggaggta atccatctcg tagaggtgct cgcgccactt gcggtccagc
    10741 accgacatga ccacgcggcg ctcgatctcg cgcatgttct cctcgcccag caactcctgg
    10801 cgctggtcgt aggcgtggtg ggcgtcgctg gtcagctcct cgaggagaac gtcccgcgag
    10861 accgccgacc ggccaccggc gcgggtggcc acgtcctcga cggtgatcga cacggggaag
    10921 agggtgccca gggccgtcca caactggtcg aggtcccacg tgtcggagta gccctcggcc
    10981 gtggccgagt tgacataggc ggcaacgacg tcgttgatga agtgccggat ctgctcttcc
    11041 atgtcggcgc cttcgagcac ctgacggcgc tcggcgtaga tgacctggcg ctgccggttg
    11101 aggacgtcgt cgtatttgag gacgtttttg cggatctcgt agttctgcgc ctcgacctgg
    11161 ctctgggcgc ccgcgatcga ac
//
LOCUS       DAOMZY010000078        11176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5451_length_11176_cov_6.287294, whole genome shotgun sequence.
ACCESSION   DAOMZY010000078 DAOMZY010000000
VERSION     DAOMZY010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 11176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11176
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5451_length_11176_cov_6.287294"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2217
                     /locus_tag="PLK64_06105"
     CDS             <1..>2217
                     /locus_tag="PLK64_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HPV79349.1"
                     /translation="GDPALDEEAAAAEAEAAEEATRAATVPSFGLLFQAPVVVTPRRR
                     ATRPSGTPALPVAAADAEAQDTLVLADELEADDIEVGAADADVLTDTDADDAVDDATS
                     PAARRRRRRGGKGRRGRLTDGPSTAGEGAAAAPAPAAGDAPDTATASGPADGSGDEGD
                     GDTDVRSDLASRRRTRRRRRGSSAGEVEDAPGVVTRVREVRSARDEVVGVRGSTRLEA
                     KKQRRREGREAGRRRQVITEAEFLARRESVERVMVVRAKEGRTQIGVLEDNVLVEHYV
                     SRETNASMAGNIYLGKVQNVLPSMEAAFVDIGRGRNAVLYAGEVNWDAAGMEAGASKR
                     IENALESGQSVLVQVTKDPIGHKGARLTSQISLPGRYLVYVPDSSMTGISRKLPDTER
                     ARLKRILRDVVPESAGVIVRTAAEGASEEELRADVERLTAIWEQIKAKAEAAGSGKKG
                     KGNSPVLLHGEPDLTVRVIRDVFNEDFAQMIVAGDEAWAQINDYVGSVAPDLASRLTK
                     WTGERDIFSNYRIDEQLAKAMDRKVWLPSGGSLVIDRTEAMTVIDVNTGKFVGAGGNL
                     EETVTRNNIEAAEEIVRQLRLRDIGGIIVIDFIDMVLESNRDLVVRRLLECLGRDRTK
                     HQVAEVTSLGLVQMTRKRVGSGLIEVFSQPCEHCNGRGYIIQSEPVERSGSTPSEPEA
                     PAPNSGNTPRSRSRRGRSGMPAEQSQPQIATLPAPAPGGPTPAQIAAAAHAAALKST"
     assembly_gap    2218..2317
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2318..2838
                     /locus_tag="PLK64_06110"
     CDS             <2318..2838
                     /locus_tag="PLK64_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79350.1"
                     /translation="PTPAQIAAAAHAAALKSTGGPESDDVDPGAVQESATTDDFAPAD
                     AAAEAYAPTDAFVGDAAGDAAGADEADVHGGATAEADTDADEGTKSAEAEAEAHTDTE
                     ASTDTDSDDASEAAGEAGADAGTDAEAGAPAAASGGRGASQGTRRGSRGRRRGRGRAS
                     APAGPPRPGQDG"
     gene            2986..3315
                     /gene="rplU"
                     /locus_tag="PLK64_06115"
     CDS             2986..3315
                     /gene="rplU"
                     /locus_tag="PLK64_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPV79351.1"
                     /translation="MEESYDVYAIVRAGGRQEKVAVGDVLLIDKVDNGEAGATLTLPV
                     LLLVDGDAVTTDAAKLAKVTVSAEVVKPAKGPKIIILKHKNKTGYRKRQGHRQPLTQV
                     KITGIQA"
     gene            3338..3607
                     /gene="rpmA"
                     /locus_tag="PLK64_06120"
     CDS             3338..3607
                     /gene="rpmA"
                     /locus_tag="PLK64_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPV79352.1"
                     /translation="MAHKKGASSTRNGRDSNAQRLGVKRFGGQVVSAGEILVRQRGTH
                     FHPGEGVGRGGDDTLFALVAGAVKFGTKRGRKTVNIEAVETVDAS"
     gene            3741..5321
                     /gene="obgE"
                     /locus_tag="PLK64_06125"
     CDS             3741..5321
                     /gene="obgE"
                     /locus_tag="PLK64_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPV79353.1"
                     /translation="MATEFVDRATLHVTAGDGGHGVASIKREKFKPLGGPDGGNGGKG
                     GSVILRVDPQATTLLDYHRSPHRRAANGKPGGGDERNGAEGDDLILPVPEGTVLKDLD
                     GTVVVDLLGMGTEHIVARGGRGGLGNKALASPRRKAPGFALLGEPGETADIVLELKTL
                     ADVGLIGFPSAGKSSLVSVLSAARPKIADYPFTTLVPNLGVVTAGSERYTIADVPGLI
                     PGAHEGKGLGLEFLRHIERCSVLVHVIDCATLEPGRDPMTDLDVIERELALYVPDAAL
                     GGRPLAERTRIIVLNKADVPEALELAELVRPDLEARGLEVHIVSAVARTGLKELSFSL
                     ARHVRDARAVVEAIVQPRIVLRPKAIDDSGFSIRKERTPEGETAYRVVGERPTRWVRQ
                     TDFTNDEAVGYLADRLQRLGVEEALYKAGAVAGDSVLIGDLDNAVVFDWQPTLSSGAE
                     LLAGPRGTDLRMEDHSRPSREEKRERMRNKYAARALTQAELNAERRAGHWATAGEAGS
                     AGSADAADPSTANPDSEA"
     gene            5321..5755
                     /locus_tag="PLK64_06130"
     CDS             5321..5755
                     /locus_tag="PLK64_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HPV79354.1"
                     /translation="MSDQFNDWNQRIIDTFRANAGEVPQFGRSLVLVHHTGAKSGLER
                     VSPVMALRDGDDTWLIAASKAGAPENPAWYHNLRANPEAEIETPDGVVLVRAEELVGA
                     DRDAAWARFTAVSTGFAEYEAKTTRTIPVLALKRRSEAATSA"
     gene            complement(5859..6485)
                     /locus_tag="PLK64_06135"
     CDS             complement(5859..6485)
                     /locus_tag="PLK64_06135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HPV79355.1"
                     /translation="MTIWDDIATERLGIAEVLEGLTEAQWQTPSLCGKWSVRELAGHL
                     VVPFHTSIPNFAVAMVKARGSFHRANDSLARAQSRRPTADLIADLRANANHHFTPPRQ
                     GPLAPLTDALVHGQDLRIPLGINDPRDPDKWQQSLDFLVTPAARAGFTRAELPPVEFV
                     ATDLDWHFGEGPQVSGPAYAIALALTTRAASLERLSGAGIDDLTRWMG"
     gene            6569..7678
                     /gene="proB"
                     /locus_tag="PLK64_06140"
     CDS             6569..7678
                     /gene="proB"
                     /locus_tag="PLK64_06140"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HPV79356.1"
                     /translation="MPRTAVARARRLVVKVGSSSLTTPRGGLDPKRLLALVDALAERH
                     GRDTQLVLVSSGAIAAGMGPLGLARRPRDLATQQAAASVGQGALIAAYQQAFGAHTIT
                     VGQVLLTIDDVTRRTHYLNARRTLERLLDLRVLPVVNENDTVATHEIRFGDNDRLAAL
                     VALLIQADALVLLSDVDALYDGPPSQPGAQRIPIVRGAHDLADVVVAGAGSQVGTGGM
                     ATKVEAAAIANAAGIPTLLTATDRVSDALRGKDVGTVFLPGSRRPPSRLLWLAHATAP
                     HGQLVLDDGAVHAVVTRRKSLLPAGIVRVEGTFVGGDPVDICDLHGKAVARGIVNYGS
                     DELPALLGRSTRDLARELGPEYQREVVHRDALVVL"
     gene            complement(7675..8670)
                     /locus_tag="PLK64_06145"
     CDS             complement(7675..8670)
                     /locus_tag="PLK64_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPV79357.1"
                     /translation="MTTKATTRHTPGVPRTPRVAWQVKFAILVLIWGSSFLFMKVGLE
                     AMTPIQIATMRVLTGMVVVLALLAMTGGRLPTTWRVWRHMIVIGFFLSAFPFTLFAIS
                     ETRVSSALAGIGNSTTPIATVLATMVLIPNARTSTRKIVAVLIGFVGVVLIAQPWTVA
                     ERPDLVGFAMAVVGGASYGVGWTYYHRFLSGADLGGLSQPAAVLVVGAGFLVPASLVV
                     WWLDRGHQATPWATHGTANGFQAWLPLLAVIALGALGTGLAFMFQYDVVRAAGPVVGS
                     TITYLIPIVSVLLGTLVLGEHLSWSAVAGFVVVLGAALVINAPERRAESEPVASA"
     gene            8758..10050
                     /locus_tag="PLK64_06150"
     CDS             8758..10050
                     /locus_tag="PLK64_06150"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HPV79358.1"
                     /translation="MVDRTAPDASVTALVAELAGRAKTASRRLALLSRADKDAALHAI
                     ADALDAAIPAVVQANADDLTQGRERGMAEGLLDRLMLDEARIRAIAEAVREVAALPDP
                     VGEVVRGSTLANGLQIRQVRVPMGVVGMIYEARPNVTVDAAALGLKSGNAVILRGGSA
                     AAASNRALVAVIRAALAERGLPEDAVTLLDEGGHDAARALMTARGLVDLVIPRGGAEL
                     IRTVVMESTVPVIETGIGVNHVYVDAAADLDKALALVMNSKTHRPSVCNAAESLVVHE
                     SVADEFLPKALTALAGAGVVLHVAPDVTRHAEAAGAAYQLASEADYATEWHRLEMNVR
                     TVADLDEALEHIRVNGSMHTEVIVTESRSAARRFVAEVDAAVVMVNASSRFTDGGQFG
                     MGAEIGISTQKLHARGPMALPELTTTKWIVDGDGQVRA"
     gene            complement(10073..11029)
                     /locus_tag="PLK64_06155"
     CDS             complement(10073..11029)
                     /locus_tag="PLK64_06155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPV79359.1"
                     /translation="MTAEPSTERPELSVVIPMFNEQEVLPLLAERLRPVLDGLTDHYE
                     VVCVDDGSSDLTPALLHKLSREWTQVRVIRLRANAGHQAAISAGLARARGDYVVTIDA
                     DLQDPPEVIAEMLQAARRDGVDVVYGVRSDRSTDSVFKRTTARMFYSFIRRISGTTAQ
                     SDAGDFRLMSRATVEAINSLPDTNRVLRFVVPALGFPSATVTYRREERAAGTSKYPFL
                     KMLTLSIDSVTGFSLFPLRLATYLGLIGGVLMFVASIVLVVASRTPGTTIPGWTSTVV
                     IVAGVGAVQLFCLGLLGEYVGRMYTHLQGRPAYFIASDSGAD"
BASE COUNT         1803 a         3902 c         3866 g         1505 t
ORIGIN      
        1 cggcgacccg gccctcgacg aggaggcagc cgcagccgag gcggaggcag cggaggaagc
       61 aacccgggcg gccacagtgc cgagcttcgg actgctcttc caagcaccgg tcgtcgtcac
      121 cccgcggcgt cgtgccaccc ggccgagcgg aactcccgcg cttccggttg ccgccgctga
      181 cgctgaagcc caggacaccc tggtcctggc cgacgagctg gaggcagacg acatcgaggt
      241 cggcgccgcc gacgctgacg tcctgaccga taccgatgcc gatgacgccg tcgacgacgc
      301 caccagcccc gccgcccgtc ggcgtcggcg ccggggcggc aagggccgcc gcggtcgcct
      361 gaccgacggc ccgagcaccg cgggcgaggg cgccgcggct gcaccagcac ccgccgctgg
      421 cgacgcaccg gacaccgcga cggccagcgg gccggctgac ggatccggcg acgagggcga
      481 cggtgacacc gacgtccgca gcgacctggc cagccgccgc cgcacgcgtc ggcgccggcg
      541 cgggtccagc gccggcgagg tcgaagatgc tcccggcgtg gtcactcgcg tccgcgaggt
      601 ccgctccgcc cgcgacgagg tcgtcggtgt ccggggttcg acccgcctgg aggccaagaa
      661 gcagcgtcgt cgcgagggcc gcgaggccgg gcgtcgccgt caggtcatca ccgaggccga
      721 gttcctcgcc cgccgcgaga gcgtcgagcg ggtcatggtc gtgcgggcca aggaaggccg
      781 cacccagatc ggtgtcctcg aggacaacgt cctcgtcgag cactacgtct cccgcgagac
      841 caacgcgtcg atggcgggga acatctacct cggcaaggtc cagaatgtcc tgccctccat
      901 ggaggccgcc ttcgtcgata tcgggcgtgg ccgcaacgcc gtcctgtatg ccggtgaggt
      961 caactgggac gcagcgggca tggaggccgg tgcctccaag cggatcgaga acgccctgga
     1021 gtcgggtcag tcggtcctcg tccaggtgac caaggacccc atcgggcaca agggcgcgcg
     1081 gctcacctcg cagatctccc tgccgggccg ttacctcgtc tacgtcccgg acagctcgat
     1141 gaccggcatc tcgcgcaagc tgcccgacac cgagcgggcc cggctcaagc ggatcctgcg
     1201 tgacgtcgtt cccgagtcgg ccggcgtcat cgtccgcacg gccgccgaag gtgcctcgga
     1261 ggaggagctt cgggccgacg tcgagcgcct cacggcgatc tgggaacaga tcaaggccaa
     1321 ggccgaggca gccggcagcg gcaagaaggg caagggcaac tcgcccgtcc tcctgcacgg
     1381 cgagcccgac ctcacggtgc gcgttatccg ggacgtgttc aacgaggact tcgcccagat
     1441 gatcgtcgcc ggcgacgagg cctgggccca gatcaacgac tacgttggct cggtcgctcc
     1501 ggacctcgcg tcccgcctga ccaagtggac cggtgagcgc gacatcttca gcaactaccg
     1561 gatcgacgag cagttggcca aggccatgga ccgcaaggtc tggctgccct cgggtggctc
     1621 gctggtcatc gaccgcaccg aggcgatgac ggtcatcgac gtcaacactg gcaagttcgt
     1681 cggcgccggt ggcaacctcg aagagacggt caccagaaac aacatcgagg ccgccgagga
     1741 gatcgtccgc cagctgcgcc tgcgcgacat cggcgggatc atcgtcatcg acttcatcga
     1801 catggtcctg gagtccaacc gcgacctggt cgtgcggcgt ctgctcgagt gcctcggccg
     1861 cgaccggacc aagcaccagg ttgccgaggt cacctcgctc ggcctggttc agatgactcg
     1921 caagcgggtc gggtccgggc tcatcgaggt gttctcccag ccgtgtgagc actgcaacgg
     1981 gcggggctac atcatccagt ccgagccggt cgagcgctcc ggttccaccc cgagcgagcc
     2041 tgaggctccg gccccgaaca gcgggaacac cccccgctcg cggtcgcgtc gcggtcgatc
     2101 cggtatgccg gccgagcagt cccagcccca gatcgccacc ctgccggccc cggcgccggg
     2161 tggcccgact ccggcgcaga ttgccgcggc agcccatgcc gctgccctca agagcacnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcc cgactccggc gcagattgcc
     2341 gcggcagccc atgccgctgc cctcaagagc accggtggcc cggagtccga cgacgtcgac
     2401 cccggcgcag tccaggagtc ggcgacgacc gatgacttcg cccctgcgga tgctgccgcc
     2461 gaagcctacg cgcctaccga cgctttcgtt ggcgacgctg ctggcgacgc agccggtgct
     2521 gacgaagcgg acgttcacgg tggcgctacg gctgaggccg acacggacgc tgacgagggc
     2581 acgaagtccg ccgaggctga agctgaggct cacacggaca ctgaggccag caccgacacc
     2641 gactctgacg atgcctcgga ggcagccggc gaggccggcg ccgacgccgg gacggacgcc
     2701 gaggcagggg ctccggccgc cgccagcggt gggcggggcg ccagccaggg cacccgtcgg
     2761 ggcagccgcg gccgacgccg tggtcgcggt cgggcctcgg caccggccgg ccctccgcgt
     2821 cccggccagg acggctaagg cagccgcgag agctggccga accgatggtt tggccaggct
     2881 cgcgcggccc ggtagagtag acccttggtg cgcccaccga tctgtgcccc tgcgtccccg
     2941 caggctggct acgtgacggt tcccgagagc gcgtgccctc gtgagatgga agagagttac
     3001 gacgtgtacg cgattgtgcg tgctggcgga cgccaggaaa aggtcgccgt tggcgatgtg
     3061 ctgctgatcg acaaggtgga caacggcgag gcgggcgcga cgctcaccct cccggtgctg
     3121 ctgctcgtcg acggcgacgc agtgaccacc gacgcggcca agctggccaa ggtgaccgtc
     3181 tcggccgagg tcgtcaagcc cgccaagggc cccaagatca tcatcctcaa gcacaagaac
     3241 aagaccggct accgcaagcg tcaggggcac cgtcagccgc tgacccaggt caagatcacc
     3301 ggcatccagg cctgagcggc agagagaagg cactgacatg gcacacaaga agggtgcgtc
     3361 ctcgacccgc aacggtcgcg actccaacgc acagcgactc ggcgtcaagc gcttcggcgg
     3421 ccaggtggtc agcgcgggcg agatcctcgt ccgtcagcgt ggcacgcact tccaccccgg
     3481 tgagggtgtc ggccgtggcg gagacgacac gttgttcgcc ctcgtcgccg gcgccgtgaa
     3541 gttcggcacc aagcgcggtc gcaagaccgt caacatcgaa gccgtcgaga ccgtcgacgc
     3601 gtcgtagtcc acagacgcac cgaggggcgg gccggatgac ggctcgcccc tcgggcgttg
     3661 ttccacccga tgaggtatgc cgcgcggcat accatccgat gacagcccac cacccaaccc
     3721 ggtgaccgta ggaggccccc atggcgacgg agttcgtcga ccgagccacc ctgcacgtca
     3781 ccgctggtga cggcgggcac ggcgtcgcat cgatcaaacg cgagaagttc aagcccctcg
     3841 gcgggcccga cggcggcaac ggcgggaagg gcggctcggt catcctgcgc gtcgacccgc
     3901 aggcgacgac gctgctggac taccaccgca gcccgcaccg acgcgccgcg aacggcaaac
     3961 ccggtggtgg cgacgagcgc aacggcgccg agggtgacga cctgatcctg ccggtcccgg
     4021 aaggcaccgt cctcaaggac ctcgacggga ccgtcgtcgt cgacctgttg ggcatgggca
     4081 ccgagcacat cgtcgctcgg ggtggccgcg gggggctggg caacaaggcg ttggcatcgc
     4141 cgcggcgcaa ggcccccggt ttcgccctgc tcggcgagcc gggcgagact gccgacatcg
     4201 tgctcgagct caagacgctc gccgacgtcg ggctcattgg cttcccgtcc gcgggcaagt
     4261 cctcgctcgt gtcggtgctg tctgccgcgc ggccgaagat cgccgactat ccgttcacga
     4321 ccctggtgcc caacctcggc gtcgtgacgg cggggtcgga gcgctacacc atcgccgacg
     4381 tgcccggcct cattccgggg gcgcacgagg gcaagggcct agggctggag ttcctgcggc
     4441 acatcgagcg gtgctcggtg ctcgtgcacg tcatcgactg cgcgacgctt gagccggggc
     4501 gcgacccgat gaccgacctt gacgtcatcg agcgcgagtt ggcgctctat gtacccgacg
     4561 cggcattggg tggccgaccg ctggccgaac gcacccggat catcgtgctc aacaaggccg
     4621 atgtccccga agcgttggag ttggctgagt tggtgcgccc cgacctggag gcgcgggggc
     4681 tcgaggtgca catcgtctcg gcggtcgctc gcaccggttt gaaggagttg tcgttctcgc
     4741 tcgcccgtca cgtgcgtgat gctcgggccg tggtcgaagc gatcgtccaa ccgcgaatcg
     4801 tgctgcggcc caaggcgatt gatgactccg ggttctcgat ccgcaaggag cgcacgcccg
     4861 agggcgagac ggcataccga gtggtggggg agcggccgac ccgttgggtg cgccagaccg
     4921 acttcaccaa cgacgaggcg gtgggctacc tcgccgaccg gttgcagcgg ctcggggtcg
     4981 aggaagcgct ctacaaggcg ggcgcggtgg cgggcgacag cgtcctcatc ggtgacctgg
     5041 acaacgcggt cgtcttcgac tggcagccga ccttgtcctc cggcgctgaa ttgctcgccg
     5101 gaccccgcgg caccgacctg cgcatggagg accactcgcg gccctcgcgc gaggagaagc
     5161 gtgaacggat gcgcaacaag tatgccgcgc gcgccctcac ccaggccgag ctcaacgccg
     5221 agcgccgggc cgggcactgg gcgacggccg gcgaggcggg cagtgctggc tctgccgacg
     5281 cggccgatcc cagcactgca aaccctgatt cggaggcgtg atgagcgacc aattcaacga
     5341 ctggaaccag cggatcatcg acacgttccg ggccaatgcc ggtgaggtgc cgcagttcgg
     5401 gcgttccctc gtgctcgtcc atcacaccgg cgccaagagc ggccttgagc gggtcagccc
     5461 ggtcatggcc ctgcgcgacg gcgacgacac ctggctgatc gccgcgtcca aggccggcgc
     5521 tccggagaac ccggcgtggt accacaacct gcgcgccaac cccgaggccg agatcgagac
     5581 ccccgacggc gtggtgctcg tgcgcgccga ggagctggtc ggcgctgacc gtgacgccgc
     5641 ttgggcgcgg ttcaccgccg tcagcaccgg gttcgcggag tacgaggcga agaccacccg
     5701 cacgatcccc gtgcttgcgc tgaagcggcg cagcgaggct gctaccagcg cctgaccggc
     5761 cagaattcct cacactcgcc gatgttctgt gcgacgccag gcccgggggt gcccgattcc
     5821 ctcacactcc ccggtgatct cggatcagcc agccccggtc aacccatcca ccgggtgagg
     5881 tcgtcgatgc ccgcgccgga caggcgctcc agcgaggccg cgcgcgtcgt gagagcgagg
     5941 gcgatcgcgt atgccggtcc gctcacctga gggccctcgc cgaagtgcca gtcgagatcg
     6001 gtggcgacga actcgacagg tggcaactcg gcccgcgtga atccggcgcg agcggcagga
     6061 gtgaccagga aatccagcga ctgctgccac ttgtcggggt cgcgagggtc gttgataccc
     6121 aaagggattc gcagatcctg cccgtgcacc aaagcatcgg tcagcggtgc gagtggccct
     6181 tggcgcggcg gggtgaagtg gtggttggcg ttggcccgca ggtcggcgat gaggtcggcc
     6241 gtcggccgcc gcgactgagc ccgagcgagc gagtcgttgg cccgatggaa gctgcccctc
     6301 gccttgacca tggccacggc gaagttgggt atcgacgtgt ggaacggcac cacgaggtgc
     6361 cccgccaact cgcggacgga ccacttcccg cacaggctcg gtgtctgcca ctgggcctcc
     6421 gtcagcccct ccaacacctc ggcgatcccc aaccgctcag tagcgatgtc gtcccagatg
     6481 gtcatgctcg cagcctacgg tcttcggtca tttcgggccg cggatcgcgc ggacgcgcca
     6541 ggtgccgccc tcgctagact cgtctgccgt gccccgaacc gccgttgccc gggcccgtcg
     6601 cctcgtggtc aaggtcgggt ccagctcgct gaccactccc cgtgggggtc tcgaccccaa
     6661 gcgcctgctc gccctcgtcg acgccctcgc cgaacgccac ggccgagaca cccagctcgt
     6721 cctggtgtct tcgggagcca ttgccgcggg catgggcccg ctggggttgg ccaggcgccc
     6781 ccgtgatctg gcgacccagc aggcggcagc cagcgtgggg cagggagcgc tgatcgcggc
     6841 ataccaacag gcgttcggtg cccacacgat cacggtcggt caggtgctgc tcaccatcga
     6901 tgacgtgacg cgacgcaccc actacctcaa cgcgcggcgc acgctggagc ggctgctcga
     6961 cctgcgggtc ctgccggtcg tcaacgagaa cgacaccgtg gccacccacg agatccgctt
     7021 cggcgacaac gaccggctcg ccgccctggt cgccctgctc atccaggccg acgcgctcgt
     7081 cctgctctcg gacgtcgatg cgctctatga cggacctccg agccagcctg gcgcgcaacg
     7141 gattccgatc gtccgagggg cgcacgacct cgccgatgtc gtcgtcgctg gcgctggcag
     7201 ccaggtgggc accgggggga tggcaaccaa ggtggaggcc gcggcgatcg ccaacgcggc
     7261 cggcataccg actctgctca cggcgaccga ccgcgtgtct gacgcgctgc gcggcaaaga
     7321 cgttgggacg gtcttcctgc cgggatcacg caggccgccg tcgcgcctgc tctggttggc
     7381 ccacgcgacc gctccccatg gtcaactcgt gctcgacgac ggcgcggtcc acgcggtcgt
     7441 cacccgccgc aagtcgttgc tcccagccgg cattgtccgc gtcgagggga ccttcgtcgg
     7501 cggcgacccc gtcgacatct gtgacctgca cggcaaggcg gtcgcgcgcg gcatcgtcaa
     7561 ctacggctcg gacgagttgc cggcgctgct gggccgctcg acccgcgacc tggctcgtga
     7621 gcttggcccg gagtatcagc gcgaggtcgt ccaccgggac gcgctcgtcg tcctctaggc
     7681 ggacgccacc ggctccgact cggcccgccg ctcgggcgcg ttgatcacca gcgccgcgcc
     7741 cagcaccacg acgaagccgg ccacggccga ccagctgagg tgctcgccga ggacgagggt
     7801 gccgagcagg acggacacga tcgggatgag ataggtgatc gtcgacccga cgaccggccc
     7861 ggcggccctc accacgtcgt actggaacat gaacgccaat ccggtcccca gcgcgccgag
     7921 cgcgatgacg gccagcagcg gcaaccaggc ctggaaccca ttggcagtcc cgtgagtcgc
     7981 ccagggagtc gcctggtgcc cccggtcgag ccaccagacc accagcgagg ccgggacgag
     8041 gaacccggcg cccaccacca gcaccgcggc cggctgcgac agcccgccca gatcggcacc
     8101 ggagaggaac cggtggtagt aggtccagcc gacaccgtag gacgccccac cgacaacggc
     8161 catcgcgaag cccacgaggt cgggacgctc ggcgaccgtc cacggctgcg cgatgaggac
     8221 gacgccgacg aaaccaatga ggaccgcgac gatcttgcgg gtcgacgtgc gcgcgttcgg
     8281 gatgagcacc attgtggcca gcacggtcgc gatgggcgtc gtcgagttgc cgatgccggc
     8341 cagcgccgag gagacgcggg tctcggagat cgcgaaaagc gtgaacggga acgccgacag
     8401 gaagaacccg atgacgatca tgtgccgcca cacccgccac gtggtcggca gccgcccgcc
     8461 ggtcatggcc agcagcgcca ggaccaccac catcccggtg agcacgcgca tcgtggcgat
     8521 ctggatcggg gtcatcgcct cgagcccgac cttcatgaac aggaaactcg acccccagat
     8581 gaggacaagg atcgcgaact tgacctgcca cgccacccgg ggggtgcgtg gaaccccagg
     8641 ggtatgccgt gtggtcgcct tcgtcgtcac cgagacagtg tgagccatgc agagacaacg
     8701 ctgcgcaccc gtccggtgtg agcgggaggt gggcgagggc ggcataccct tggacgcatg
     8761 gttgaccgca ccgcgcccga cgcctccgtg accgctctcg ttgccgagtt ggccgggcgc
     8821 gccaagaccg cctcgcgccg tctcgcgctg ctctcgcgcg ccgacaagga cgccgcgctg
     8881 cacgccatcg ccgacgcgct cgacgcggcg atcccggcgg ttgtccaggc caacgctgac
     8941 gacctgaccc aagggcgcga gcggggcatg gccgagggac tgctcgaccg gctcatgctc
     9001 gacgaggccc ggatccgggc gatcgccgag gcggtccgtg aggtcgccgc gctgcccgac
     9061 ccggtcggcg aggtggtccg cggctcgacc ctggccaacg gcctgcagat ccgccaggtg
     9121 cgggtgccga tgggcgtcgt cgggatgatc tacgaggcgc gacccaacgt caccgtcgac
     9181 gccgccgcac tcgggctcaa gtcgggcaac gccgtcatcc tgcgcggtgg ctcggcggcc
     9241 gccgcgagca accgggcgct cgtcgccgtc atccgcgcag ccctggccga gcgcggcttg
     9301 cccgaggacg ccgtcaccct gctcgacgaa ggcggccacg acgctgcccg cgcgctgatg
     9361 accgcgcgcg gactggtcga cctggtcatc ccgcgcggcg gcgcggaact catccgcacc
     9421 gtcgtcatgg agtcgaccgt cccggtcatc gagacgggga tcggcgtcaa ccacgtctat
     9481 gtcgacgcgg cggccgacct cgacaaggcg cttgcgctgg tgatgaactc caagacgcac
     9541 cggcccagcg tctgcaacgc cgccgagtcc ctcgtggttc atgagtcggt ggccgacgag
     9601 ttcctgccta aggccctcac cgcgctcgcg ggcgccgggg tggtcctgca cgtcgctccc
     9661 gacgtgaccc ggcatgcgga ggcggcgggc gcggcatacc aactggcgtc cgaagcggac
     9721 tacgcgaccg agtggcaccg gctggagatg aacgtccgca cggtcgcgga tctcgacgag
     9781 gcgctggagc acatccgggt caacgggtcg atgcacaccg aggtgatcgt caccgaaagt
     9841 cgttcggcgg caaggcggtt cgtcgccgag gttgacgcag cggtggtcat ggtcaacgcg
     9901 tccagccgct tcaccgacgg cgggcagttc gggatggggg ccgagatcgg catctccacc
     9961 cagaagctgc acgcccgcgg cccaatggcc ctgcccgagc tcacgacgac caagtggatc
    10021 gtcgacggcg acggccaggt gcgcgcgtag cggtcagtcg gcgccggagc gctcagtcgg
    10081 cgcccgagtc ggaggcgatg aagtatgccg gtcgcccctg caggtgggta tacatccgtc
    10141 cgacgtactc accgagcagg cccagacaga acagctgaac cgcgccgacc cccgcaacga
    10201 taacgacggt cgaggtccaa ccggggatgg tcgtgcccgg tgtccgcgac gcgacgacca
    10261 ggacgatcga ggcgacgaac atgaggacgc cgccgatcaa cccgagatag gtcgcgaggc
    10321 gcagcgggaa aagggagaag ccggtcaccg agtcgatcga cagggtgagc atcttgagga
    10381 acgggtactt cgacgtgccc gcggcccgct cctcgcgtcg ataggtgacc gtcgccgacg
    10441 ggaaacccag cgccggcacg acgaagcgca gcacccggtt ggtgtcagga aggctgttga
    10501 tggcctcgac ggtggcccgc gacatgaggc ggaagtcacc tgcgtcggac tgcgccgtcg
    10561 tgcccgagat ccgccggatg aaggagtaga acatccgcgc cgtggtccgt ttgaagaccg
    10621 agtcggtgga tcgatcagag cgcacgccat agacgacgtc gaccccgtcg cgccgggcgg
    10681 cctgcagcat ctcggcgatc acttccgggg ggtcctgcag gtcggcgtcg atcgtcacga
    10741 catagtcgcc gcgggctcgc gccagccccg ccgagatcgc cgcctggtgc ccggcgttgg
    10801 cccgcagccg gatcactcgc acctgggtcc actcgcggga cagcttgtgc agcagggccg
    10861 gggtgaggtc gctgctgccg tcgtcgacgc agacgacctc gtagtgatcg gtgagcccgt
    10921 cgagcaccgg tcgcaggcgc tccgcgagca gcggcagcac ttcctgctcg ttgaacatgg
    10981 ggatgaccac cgagagttcg gggcgctcgg tgctcggttc cgcggtcatg gtgccaaggg
    11041 tacgggccgg gacctggccc acctgcgagg ccgctgtgca ggccccatga agggcagtcc
    11101 gcgggcgcgc ggacgggggc gggcggcata cctcacaccg tggagacagc caagagggta
    11161 tgccgcgcgc acgggg
//
LOCUS       DAOMZY010000079        11110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5494_length_11110_cov_6.749706, whole genome shotgun sequence.
ACCESSION   DAOMZY010000079 DAOMZY010000000
VERSION     DAOMZY010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 11110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11110
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5494_length_11110_cov_6.749706"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..1833)
                     /locus_tag="PLK64_06160"
     CDS             complement(166..1833)
                     /locus_tag="PLK64_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV79360.1"
                     /translation="MTTPVTDSPVLSIRNLSIGFRAGGKVAPAVRDVSLDVGVGETVA
                     IVGESGSGKSTTAAAVNGLLPDNAVITSGSILFDGSDIRALTEKQLIAVRGAGIGLVP
                     QDPMSNLNPLMRIGRQIGEALEVHGRTTGRATDQRVHELLELVGIPDPERRATQYPHE
                     FSGGMRQRVLIAIGLSCNPRLLIADEPTSALDVTVQRRILDQLESLTVGSGTAVFLIT
                     HDLALAAERADRVAVMYRGEIVEQGPSKQVLRSPSHDYTKSLIAAVPGLSLGRAPVRT
                     AARERISTSDVPLVSVRHVVKEYALRGGSAESFKAVDDVSFDIRAGSTVSVVGESGSG
                     KSTTANMVLGLETITSGNIVFEGNDLTTMRGKELFALRRRVQPVFQNPYSSLDPRFTI
                     ARTIAEPLAVHKVGDPTSRRQRVLELLDQVALPPAVADRYPHELSGGQRQRVAIARAL
                     ALRPDLVVLDEAVSALDVLVQAQILELLASLQREMGLTYLFISHDLAVVRMISDEVHV
                     MRRGKIIESGAPDQLFDHPAHEYTQELIAAIPGRSVASARLGEAEPA"
     gene            complement(1830..2963)
                     /locus_tag="PLK64_06165"
     CDS             complement(1830..2963)
                     /locus_tag="PLK64_06165"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPV79361.1"
                     /translation="MNASTGSELLDAVGTAAHAVHALASGPLGVTIGVTTGAGTRIKS
                     VGAADGNHRALHTDTRFDIASVTKVVATTTAIHRLASVGVLRLDDRVDRFVADSPCTS
                     DATISTLLTHRAGLWEWQPLYLAPSADGRPVDAYQAVAALPLRYPPGERRAYSDLGFI
                     LLGRIIERATGLSLAEAVSEFVTGPLGLVDTAYGPVTGDVAASAPGDGVERDMVGSGD
                     PYPVLYPSREIAWRTHELTGEVNDGNCYRVLGGVSGHAGMFSTAVDLLRLGRSLALAA
                     TSDDLWRPDITAQIFAEGPDSGQALGWRTMPVLLGGVRRNLLWHPGFTGCALGLVPGT
                     GVALVLLSNRLMSAAPLPTAQLWATALSSLGVSDAPMLIGSRP"
     gene            complement(2960..3448)
                     /locus_tag="PLK64_06170"
     CDS             complement(2960..3448)
                     /locus_tag="PLK64_06170"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV79362.1"
                     /translation="MSVQLRRAGLEDRTGIVDVFLDCWRQSYRGVLPDRLTDSMTDEA
                     ATALWNRALTAPDRTVVVAVRDGVVVGVTSYGVNGSTGLVQSLYVSPSAQGLGLGRRL
                     LEKAAVDLTKAAATSATLWVFAANTPSVGFYAACGWLPDGVTRVQEEFGEPEIELHKA
                     LA"
     gene            complement(3445..4614)
                     /locus_tag="PLK64_06175"
     CDS             complement(3445..4614)
                     /locus_tag="PLK64_06175"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HPV79363.1"
                     /translation="MRVLGMISGTSHDGIDLAVVDLDADGDVLVGSVLTSDTVPYDPD
                     LRARLVAALPPAATTLSEMCQLDTLIGQAFAQAAAEAAERVGGVDAVCSHGQTVYHWV
                     EADHALGTLQLGQPAWIAERVGRPVVADVRARDLTIGGHGAPLASLLDALLLGGHAGV
                     PAALNLGGISNMTVITDGRVSAFDIGPSNALMDAVVQTEGLDPRGYDDGGRLAAGGSV
                     DVDLLDALLADPYYALPAPKSTGKEHFHGTYVREAVAALGHDISPIDLLATLTELTVR
                     TVAEAVRTAGVTYLAMSGGGCRNPLLVDGIRAALPDVEVVTTDALSVPTDAKEAILLA
                     VIGWCTLHGLPGVVAGGTGARVPRILGSITPGSGPLRLPEPASPIRSIRLHGGVT"
     gene            complement(4596..5546)
                     /gene="murQ"
                     /locus_tag="PLK64_06180"
     CDS             complement(4596..5546)
                     /gene="murQ"
                     /locus_tag="PLK64_06180"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HPV79364.1"
                     /translation="MTPAQGDDASDDSDLSALLTESVDPRYADLDILSVAQLAELMNA
                     ADRTVPDAVHAALPQIVLAVEAILPRMIAGGRLVYVGAGTAGRLGILDASECPPTFNT
                     RPEQVFAIIAGGSRAVQTAVEGAEDDPAQGAVAVDAAKVEPRDTVVGITASGRTPYVL
                     GAVKRARELGALTVGLSCDAGSALSGAVERPIEVLVGPEVLSGSTRLKSGTAQKLVLN
                     MISTIVMVRMGKTYGNLMVDVRPTNHKLRVRARRIVQQIASASGDDAAAALAASGNDI
                     RVASLMLARHESAERAAERLRDAGGNLRTALEDGQCASWA"
     gene            complement(5543..6418)
                     /locus_tag="PLK64_06185"
     CDS             complement(5543..6418)
                     /locus_tag="PLK64_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV79365.1"
                     /translation="MTADTMVGAPPRGGQRLRLLLHSRTGMLGLVLVGFLLVLAIAQA
                     FGLVGTDPLKQAASIRLQGPSAAHWFGTDQFGRDVFSRVAAGVANTTQIAAVAVGVAA
                     LVGTLLGVIAGFYLKGPDAIISGVTNVLFAFPPLLLALSLTSVMDRNWFTVAVAIAIV
                     YIPIFIRVARGPVLSLRNIEYVRAAVSTGQSRMAITMRHVLPNITSIIIVQVSLSLSW
                     AVLTEASLSFLGLGNPPPAPSLGSMIYEARTLVSVAPWTMVAPGIVIVLLVVGLNLLG
                     DGLRDSFDPRKRGSS"
     gene            complement(6415..7380)
                     /locus_tag="PLK64_06190"
     CDS             complement(6415..7380)
                     /locus_tag="PLK64_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV79366.1"
                     /translation="MTIPWRTVVRRLLSMMGTLLGVAIFVFIMLRAIPGNQITAGLGT
                     EAAALTPEQRADLEAYYGIDKPLIVQFFSWLGNIATGNLGFSSRSQVSVASLTVDALP
                     VTIELAVLSIILALLIGIPLGMLSASKPDSARDAVGQIFGLAGLSIPSFLLASALLTV
                     LSAKFGFNPNGEAYASLAEDPVRNLRQMLLPSLTLGFGIAAPIMRTTRTALLEVRSQD
                     FVRTARAKGVGNRRLQVRHILGNALVPIVTITGLQFGYLLGGAVVVEQIFSLPGIGRQ
                     VLIGIQGKEYAVVQSTVLVIALLFVVVNLLTDLLYRVIDPRVRAS"
     gene            complement(7445..8860)
                     /locus_tag="PLK64_06195"
     CDS             complement(7445..8860)
                     /locus_tag="PLK64_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPV79367.1"
                     /translation="MVGITSDPDNLFPWKATQFQAVNVLQTLYGTLTEFDKDLNVVPG
                     LAESWTYSADNLTLTMKLRAGVKFADGSACDSQDVKFSLDKIKDKATAAVAAASVASV
                     TSVEAPDANTVVLKLTAPDAALLGNLASINMAILPTDSTEAALKTTPAGTGPFILKDR
                     KPSQSITVARNPAYWGDKTPLETVEFRIIPDETSIVSAMQSNNVQLAVLNDPLVAKTA
                     QGGSLTVTKTPQLSYHVLQLNARRPALTDVNVRLAMQCAVDRQQVLDTAALGEGEVIG
                     PITSPAYRSDPSARPCASRDLAKAAEYLAKAGKSDGVSVKTIVSQGEYATSVAEAQNL
                     KAQLAEAKITLDLDVLASNAYVDRWVAGDFDAAVALNGGRPDPDGMYGRYFTSTGNLN
                     KVAGYSSPDLDALFAQGRESTDPTARKATYAKVSQHLEANAAWIWMFSGFTYTVAAPK
                     VTGYLPMANGSVQYLRSTSLP"
     gene            9170..10057
                     /locus_tag="PLK64_06200"
     CDS             9170..10057
                     /locus_tag="PLK64_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018779387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HPV79368.1"
                     /translation="MTRSEGGQSTALVTMRTLLPNLRASERRIAELFLADPEASAELA
                     IAEVAERSGTSTTSVIRLCKRLGYKGFKDLRNDVLREVTRESFETAAFAGTPRDIDRS
                     DSIEDIIAKVSLAETLSIADTTKVLDVVALGKAADALAAATRIDIFGLGASSFVGEDL
                     QQKLVRIGRAALRWPDSHAAWTAATTLGPGCVAVALSHSGATVDTISFLDLARSAGAT
                     TIAITNHSSSPLTEAADIVLTTAARETGFRSGALGSRIAQLMVIDCLFIRVAQLDFDA
                     SMEAIRNTYATIQRLTAKR"
     gene            complement(10098..10472)
                     /locus_tag="PLK64_06205"
     CDS             complement(10098..10472)
                     /locus_tag="PLK64_06205"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPV79369.1"
                     /translation="MFVTRGAEAPTFDLPGVRFTPLAAPTLGSADLCLWRLDVDPGLE
                     SPEAHVLDQDEIFVVVAGTISLSIGGMRLTAGDAAIIPAGEPIQLSNPGSEPATVHVA
                     IRSGFAATAADGTSIGTPPWAA"
     gene            10532..11005
                     /locus_tag="PLK64_06210"
     CDS             10532..11005
                     /locus_tag="PLK64_06210"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPV79370.1"
                     /translation="MAPAAQPVSDIGISVLLAYQGFVRALHADLAAHGFDDVGKSDGL
                     VFRLLSDRSRTVSELAGLLEVSKQGAGQIIQDMETRGYVVRDQSPGDARARPVRLSDR
                     GLVALAAARRFHRRTEARLVREHGRESVATFRAVLTAMAGDDATALDQQVRGLYF"
BASE COUNT         1860 a         3687 c         3832 g         1731 t
ORIGIN      
        1 ggcgccgcgc tcgaccgggc cgtcttcgat caggtcgcgg ccgcgatccg cgccgtccgc
       61 tgaccgccgc ccgctaaccg ccgcccgctg gccaggcgca cggcatacgc gaagggtcgg
      121 ctcccacacg aggagccaac ccttcgatgt atgccgcgtc gtcgcctacg cgggctcggc
      181 ctcccccagc cgcgcactcg caaccgaccg cccagggatc gcggcgatca actcctgggt
      241 gtattcgtgc gcggggtggt cgaagagctg atcgggggcc ccgctctcga tgatcttgcc
      301 gcgacgcatg acatggacct cgtcggaaat catccgcacc acggcgaggt cgtggctgat
      361 gaacaggtag gtcaggccca tctcgcgctg caacgacgcg agcaactcaa ggatctgggc
      421 ctggacgagg acatccagcg ccgacacggc ttcgtcgagg accaccaggt cggggcgcag
      481 cgccagtgcg cgtgcgatgg cgacccgctg acgttggcca ccggacaact cgtgcggata
      541 acggtcggcg acggccgggg gcagggccac ttggtccagc aactcgagca cccgctgccg
      601 gcgcgacgtg gggtcgccga ccttgtgcac cgccaggggc tcggcgatgg tgcgggcaat
      661 ggtgaaacgg gggtcgaggg aggaatacgg gttctggaag acgggctgca cccgccggcg
      721 cagggcgaac agctccttcc ctcgcatggt ggtcaggtcg ttgccctcga acacgatgtt
      781 tccgctcgtg atggtctcca gaccgagcac catgttcgcc gtggtcgact tgcccgagcc
      841 ggactccccg accaccgaca cggtgctgcc ggcgcggatg tcgaacgaga catcgtcgac
      901 cgccttgaac gactcggccg agccgccgcg caacgcatac tccttgacca cgtgacggac
      961 tgacacgagc gggacgtcac tggtggagat ccgttctctc gcagcggtcc gcaccggcgc
     1021 gcgccccagc gacagcccag gcacggcagc gatcagtgac ttggtgtagt cgtgcgacgg
     1081 actgcgcagc acctgcttgg acgggccttg ctcgacgatc tccccgcgat acatgaccgc
     1141 gacccgatcg gcgcgctcgg ccgcgagggc gaggtcgtgg gtgatgagga agaccgccgt
     1201 gcctgacccg acggtcaggc tctccaactg gtcgaggatg cgccgctgga cggtcacgtc
     1261 gagggcggat gtcggctcgt cggcgatcag gaggcgtggg ttgcacgaga ggccgatcgc
     1321 gatgagcacg cgctggcgca taccgccgga gaactcatgg gggtattggg tggcccggcg
     1381 ctctgggtcg gggatgccga ccagttccag cagttcgtga acgcgctggt cggtggcccg
     1441 gcccgtggtg cgtccatgca cctccaacgc ctcaccgatc tgtcggccga tccgcatgag
     1501 cggattgagg ttgctcatcg ggtcctgcgg caccagcccg atccccgcgc cacgcacggc
     1561 gatgagctgc ttctcggtca gcgcgcggat atcgctgccg tcgaagagaa tgctcccgct
     1621 ggtgatgacc gcgttgtccg gcaggaggcc gttcaccgcc gcagcggtgg tgctcttgcc
     1681 cgacccggac tcgccgacga tggcaacggt ctcgccgacg cccacgtcga gtgacacgtc
     1741 gcggactgcc ggtgcaacct ttccgccggc acggaacccg atcgacagat tgcgaatcga
     1801 caggacgggg ctgtcggtca cgggtgtggt catggtcggc tccctatgag catcggggcg
     1861 tcactgacgc cgagtgagga caacgcggtg gcccacagct gggccgtcgg cagcggtgcg
     1921 gcggacatga ggcggttgga cagcaagacg agggcaactc ccgtgcccgg gaccagaccg
     1981 agcgcacacc cggtgaaacc cgggtgccag agcaggttgc ggcgcacgcc gcccagcagg
     2041 accggcatcg tccgccaccc gagtgcctgc cccgagtccg gcccctcggc gaagatctgc
     2101 gcggtgatgt ctgggcgcca caggtcgtcc gacgtggctg ccagcgccag agatcgaccc
     2161 agccgcagga ggtcgacggc cgtcgagaac atcccggcgt gcccgctgac accgcccaac
     2221 acccggtagc agttgccgtc gttcacctcg ccggtcagtt cgtgcgtccg ccaggcgatc
     2281 tcgcggctgg gatagagcac cggataggga tcaccgctgc cgaccatgtc ccgctcgacc
     2341 ccgtcgccgg gtgcgctcgc tgcgacgtca cccgtcaccg gcccgtatgc cgtgtccacc
     2401 agtccgaggg gaccggtgac gaactcggac accgcctcag ccaacgacag gcccgtggcc
     2461 cgttcgatga tgcggccgag caggatgaag ccgaggtccg aataggctcg acgctccccc
     2521 ggcggataac gcaggggcag ggcggccacc gcctgatacg catcgacggg tcgaccgtcc
     2581 gcggacggcg cgaggtagag cggctgccac tcccacaggc ccgcccgatg cgtcaggagg
     2641 gtcgagatcg tggcatccga agtgcagggc gagtcggcga cgaatcggtc gacccggtca
     2701 tccagacgga gcaccccgac ggaggcaagt cggtggatcg ccgtggtggt ggccacgacc
     2761 ttcgtcaccg aggcgatgtc gaaccgcgtg tcggtgtgca gcgcccgatg gttgccgtcg
     2821 gcggcaccga ccgacttgat cctggtcccg gccccggtcg tgacgccgat ggtgacgccg
     2881 agcggccccg acgccagggc atgcactgcg tgggctgcgg tcccgacagc atcgaggagc
     2941 tccgaccccg tcgacgcgtt cacgccaacg ccttgtgcag ctcgatctcc ggttcgccaa
     3001 actcctcctg aacccgcgtc accccgtcgg gcagccaccc gcaggccgca tagaagccga
     3061 ccgacggagt attggccgcg aacacccaca gcgtcgccga ggtcgccgcg gccttcgtga
     3121 gatcgacggc agccttctcg agcaaacggc gtcccaggcc gaggccttga gcactaggcg
     3181 acacatagag cgactggacc aggcccgtgg acccgttcac gccgtaacta gtgaccccga
     3241 cgacgacgcc gtcccggacc gccacgacga cggtgcggtc cggcgcggtg agggcgcggt
     3301 tccacagcgc ggtcgccgct tcatcggtca tggaatcggt gaggcggtcg ggaagcacgc
     3361 cgcggtagct ctgccgccag cagtcgagga agacatcgac gatcccggtg cggtcctcca
     3421 gtccggcccg acgcagctgg acgctcacgt gacgcccccg tgcagtcgga tcgagcggat
     3481 cggcgacgcc ggctcgggca gccgcaaggg accactcccg ggtgtgatgc ttcccaggat
     3541 gcgcggcacg cgagccccgg tgcccccggc gacgacgccg ggcagcccat gcagcgtgca
     3601 ccagccgatc acggcgagca ggattgcctc ctttgcgtcg gtggggacgg agagcgcgtc
     3661 cgtggtcacc acctcgacgt caggcagggc agctcgtatg ccgtcaacca gcaacgggtt
     3721 tcggcacccg ccgccggaca tggcgaggta ggtcaccccg gcggtccgga cggcctcggc
     3781 cacggtgcgc acggtcagct cggtcagtgt cgcgagcaga tcgatggggc tgatgtcgtg
     3841 gccgagggca gcgacggctt ctcgcacgta ggtcccgtgg aagtgctcct tgccggtgct
     3901 cttcggcgcg ggcaacgcgt aataggggtc tgcgagcaag gcgtcgagaa ggtcgacgtc
     3961 gaccgatccg ccggcggcga gccggccccc gtcgtcgtag cctcgcgggt ccaggccctc
     4021 ggtctgcacc acggcgtcca tgagcgcgtt cgaggggccg atgtcgaagg ccgatacgcg
     4081 tccatcggtg atgacggtca tgttcgagat gccgcccagg ttcagggcgg ccggcacccc
     4141 cgcgtggcca cccaagagca gggcgtccag aagcgaggcg aggggggctc cgtgccctcc
     4201 gatcgtcagg tccctggcgc ggacgtccgc aacgaccggt cgaccgaccc gctcggcgat
     4261 ccacgccggc tggccgagtt gcagcgtccc gagtgcgtgg tcagcctcga cccagtggta
     4321 gacggtctgg ccgtgcgagc agaccgcgtc cacgccgccg accctctcgg cggcctcggc
     4381 ggctgcctgc gcgaaggcct gcccgatgag ggtgtcgagc tggcacatct ccgacagcgt
     4441 cgtcgcagcg ggtggcaggg cagcgacgag ccgggccctg aggtcggggt cataggggac
     4501 ggtgtcgcta gtgaggacac tccccaccag gacatcgccg tcggcgtcca gatcgacgac
     4561 cgccaggtcg atgccgtcgt gtgacgtccc tgagatcatg cccaagacgc gcattggcca
     4621 tcctccagtg cagtgcgtag gttcccgccg gcgtcgcgca ggcgttcggc cgcgcgctcg
     4681 gccgactcgt ggcgagcgag catgagggag gccactcgga tgtcgttccc ggaagctgcc
     4741 aaggcggctg ccgcgtcgtc gccggacgct gaagcgatct gctggacgat ccggcgggcc
     4801 cgcactcgga gcttgtggtt ggtggggcgg acgtcgacca tgaggttgcc gtaggtcttg
     4861 cccatgcgca ccatgacgat cgtcgagatc atgttgagga cgagtttctg cgccgtcccg
     4921 gacttcaggc gggtcgagcc ggagaggacc tccggcccga caagcacctc gatcggtcgc
     4981 tcgaccgcac cactcaacgc cgacccggca tcgcaggaca ggcccacggt gagcgcgccg
     5041 agctccctcg cccgcttgac cgctccgagg acgtagggcg tccggccgct ggccgtgatc
     5101 cccacgaccg tgtccctcgg ctcgaccttg gccgcatcca ccgctactgc cccttgggca
     5161 gggtcgtcct cagccccttc gactgcggtc tgcacggcgc gtgagccgcc cgcaatgatg
     5221 gcgaagacct gctcgggcct ggtgttgaag gtcggcggac actctgacgc gtcgaggatt
     5281 cccaggcgtc cggccgtgcc ggcaccgacg tagaccaagc ggcccccggc gatcatccgc
     5341 ggcaggatcg cctcaacggc aaggacgatc tgcgggagcg cggcatgaac cgcgtccggc
     5401 acggttcgat ccgcggcatt catcagctcg gccaactgcg ccaccgacag gatgtcgaga
     5461 tccgcgtatc gcgggtccac cgactcggtc aacagcgccg agaggtccga gtcgtcgctc
     5521 gcgtcgtcgc cctgcgcggg ggtcatgacg atccccgctt tcgggggtcg aacgagtcgc
     5581 gcaggccgtc gccgaggagg ttgaggccca cgacgagcag gacgatgacg attcctgggg
     5641 caaccatcgt ccacggcgcg acggacacga gggtgcgggc ctcgtagatc atcgaaccca
     5701 gggagggggc cgggggtggg tttccgagtc cgaggaagct cagcgacgcc tcggtgagca
     5761 ccgcccagga cagcgacaac gacacctgga cgatgatgat cgacgtgatg ttgggcagca
     5821 cgtgccgcat cgtgatggcc atcctgcttt ggccggtgct gaccgccgcg cggacgtatt
     5881 cgatgtttcg cagcgagagc accgggccac gcgcgacccg gatgaagatg ggtatgtaga
     5941 cgatggcgat ggccacggcg acggtgaacc agtttcggtc catcaccgag gtcagcgaca
     6001 gcgcgagaag caggggcggg aaggcgaaca acacgttggt gacgccgctg atgatggcgt
     6061 cgggcccctt gaggtagaag cccgcgatga cgcccagcag cgtccccacg agagcggcca
     6121 caccgaccgc caccgcggcg atctgggtcg tgttcgccac tccggctgcg acgcgggaga
     6181 agacgtcacg accgaactga tcggtgccga accagtgcgc tgccgatggc ccctgcagtc
     6241 ggatgctcgc ggcttgcttg agcgggtcgg tgccgaccag gccgaacgct tgcgcgatcg
     6301 ccaggaccag caggaagccg accagcacga ggccgagcat tccggtgcgg ctgtgcagaa
     6361 ggagtcgaag acgctgaccg ccgcggggcg gggcgccaac catcgtgtcg gcagtcatga
     6421 ggcacgcacc cgggggtcga tgacgcggta gaggaggtca gtgagcaggt tgacgacaac
     6481 gaagaggagg gcgatgacga gaaccgtgct ctggacgacg gcatactcct tgccttggat
     6541 cccgatgagc acctgccggc cgatgccggg gaggctgaag atctgctcca ccaccacggc
     6601 gccgcccagc aagtacccga actgcagacc ggtgatcgtg acgatcggga cgagcgcgtt
     6661 gcccaggatg tggcgcacct gcaggcgtct gtttcccacg cccttggctc gtgccgtgcg
     6721 cacgaagtcc tgggatcgca cctccaacaa ggccgtgcgc gtggtcctca tgatgggagc
     6781 ggcgatcccg aaacccagcg tgagggaggg cagcaacatc tgccgcaggt tgcggaccgg
     6841 gtcctcggcg agcgacgcat aggcctctcc gttggggttg aagccgaact tggccgagag
     6901 caccgtcaac aaggcgctgg ccaagaggaa cgacgggatg ctgaggccag cgagcccgaa
     6961 gatctgtccc actgcgtccc gggccgagtc cggtttcgac gccgagagca tccccaacgg
     7021 aatgccgatc agcagcgcca agatgatcga caacaccgcc aactcgatgg tgaccggcag
     7081 cgcgtcgacc gtcaggctcg cgacgctcac ctgtgaccgg gacgagaacc ccagattgcc
     7141 ggtggcgatg ttcccgagcc aggagaagaa ctgaacgatc aggggcttgt cgattccgta
     7201 ataggcctca aggtcggctc gctgctcggg cgtgagcgcg gcggcctcgg tgccgagtcc
     7261 cgcggtgatc tggttacccg ggatcgcgcg aagcatgatg aagacgaaga tggcgacccc
     7321 aagcagggtg cccatcatgg agaggagccg gcggacgacg gtccgccacg ggatggtcat
     7381 gggtgcctcc gaggcgtgga aagtggcggc tgcgggccca cggggtggac ccgcagccga
     7441 tccatcaggg cagcgaggtc gaacggaggt actgcacgga accgttcgcc atcgggaggt
     7501 acccggtgac cttcggcgcg gccaccgtgt aggtgaagcc cgagaacatc cagatccacg
     7561 cggcgttggc ctcaaggtgt tgcgagacct tggcgtacgt ggccttgcgg gccgtcgggt
     7621 cggtcgactc gcggccttga gcgaacagtg cgtccaggtc cggtgagctg tagccggcga
     7681 ccttgttgag gttccccgtg ctcgtgaagt agcgaccgta cataccgtcc gggtccggcc
     7741 gaccgccgtt gagggcgacc gcggcgtcga agtcaccggc cacccatcga tccacgtagg
     7801 cgttggaggc caggacatcc aggtcgaggg tgatcttggc ttcggccaac tgagccttga
     7861 ggttctgggc ctcggcgacg gaggtggcgt actccccctg cgacacgatg gtcttgacgc
     7921 tcaccccatc ggacttgccg gccttcgcca gatactcggc agccttagcg aggtcgcgcg
     7981 acgcacatgg ccgagcagac gggtcggagc ggtatgccgg tgaggtgatc ggtccgatga
     8041 cctcaccctc gccaagagca gccgtgtcga ggacctgctg gcggtcgacc gcacactgca
     8101 tggccagacg tacgttgacg tcggtgaggg cagggcgtcg ggcgttcaac tgcaggacgt
     8161 gatagctgag ttggggcgtc ttcgtcacgg tgaggctgcc accctgggcg gtcttggcca
     8221 ccagcgggtc attcaacacc gccagttgga cgttgttcga ctgcatcgcc gagacgatgg
     8281 aggtctcgtc cgggatgatg cggaactcga ctgtttccag gggtgtcttg tcgccccagt
     8341 aggcggggtt gcgggccacc gtgatggact ggctcggctt gcggtccttg aggatgaatg
     8401 gccccgtgcc ggccggcgtg gtcttcagcg ctgcctcggt cgagtctgtc gggaggatgg
     8461 ccatgttgat cgaggccagg ttcccgagca gggcggcgtc cggtgcggtg agtttgagga
     8521 ccaccgtgtt cgcatcaggt gcctcgacgc tggtcaccga cgccacggat gccgcagcaa
     8581 cggctgccgt cgccttgtcc ttgatcttgt ccagcgagaa cttcacgtcc tgggagtcgc
     8641 aggcgctccc gtcagcaaac ttcacgccgg cgcgcaactt catcgtcagg gtgaggttgt
     8701 ccgcggagta ggtccaggac tcggccaggc cgggaacgac attgaggtcc ttgtcgaact
     8761 ccgtgagcgt gccgtacagc gtctgtagga cgttgacggc ctggaactgc gtggccttcc
     8821 aggggaagag attgtcgggg tcgctggtga tcccaaccac caggctcccg ccagctttgg
     8881 acgcgttgga cgacgctgcg ccacccgtgc ctgacgagca cgcggaggca gccatagcga
     8941 aggcaaggcc gagactgatc aacggaactg agccacgcga gaggcgtgta cgacgagcca
     9001 tcagaactcc tttcgaaccg agccgttgtc gttggtcctc cgtaggctgg aggtgacgcc
     9061 gggtggtagg tcgctaactt acatgtcgac ggttcgaaat gtactagact tacctcctct
     9121 tgactagagt ctggtgacca ccaagacggg aagtcctatg accgcatcga tgacacgctc
     9181 cgaggggggg cagtccaccg ccctcgtgac catgcgcacc ctgctcccga acctgcgggc
     9241 cagtgagcga cgcatcgcgg agctcttcct cgccgacccc gaagcgtcgg ctgaactggc
     9301 catcgccgag gtcgcggagc gcagcgggac ctcaacgacc tcagtgatcc gcttgtgcaa
     9361 gcgactgggc tacaaggggt tcaaggacct gcgcaacgac gtgctgcgtg aggtcacccg
     9421 ggagtccttc gagacggcgg cgttcgccgg caccccgcgc gacatcgatc gctcggacag
     9481 catcgaggac atcatcgcca aggtctcgct cgccgagaca ctgtccatcg ccgacaccac
     9541 caaggtcctg gacgtcgtcg ccctagggaa ggccgccgat gctctcgcgg ctgccactcg
     9601 catcgacatc ttcggcctcg gcgccagttc cttcgtcggc gaggacctcc aacagaaact
     9661 ggtgcggatc gggcgagcag cgcttcgctg gcccgactcc cacgcggcgt ggaccgccgc
     9721 caccaccttg ggtccgggct gcgtggctgt cgccttgtcc cactcgggcg ccaccgtgga
     9781 caccatctcg ttcctggatc tcgcccgcag tgcgggcgcc acgaccatcg ccatcaccaa
     9841 ccacagcagc tcccccctca ccgaggccgc cgacatcgtg ctgaccacgg ctgcccggga
     9901 gaccggtttc cggtccggcg ccctgggcag ccgcattgct caactcatgg tcatcgactg
     9961 cctgttcatc cgggtggccc agctggactt cgacgcgtcc atggaggcca tccgtaacac
    10021 ctacgccacg atccaaaggc tcacggcgaa acgctagagc cagtggtcga tgcttggcgg
    10081 atcagggcgg tcggcagtca ggccgcccag ggcggcgtcc ctatcgacgt gccgtccgcg
    10141 gcggtcgcgg cgaaaccaga ccggatcgcc acatgcaccg tggccggctc cgagcccggg
    10201 ttgctcaact gaatcggctc gccggcgggg atgatcgcag cgtcgccggc ggtcagacgc
    10261 atgccaccga tcgacaggga gatcgtgcct gcgacaacca cgaagatctc gtcctgatcg
    10321 agcacgtggg cctcagggga ctccagcccg gggtcgacgt ccaggcgcca gaggcacagg
    10381 tctgccgatc ccagcgtcgg cgcggcgagc ggcgtaaagc gcactccggg caggtcgaag
    10441 gtcggcgcct cggcgcctcg agtgacaaac atgttcgctc tcctcgatag acaagttcac
    10501 ttgtccaagt gaacttgtct atcatgaggt tatggcaccc gctgcgcaac ccgtttcaga
    10561 catcggcatc tcggtcttgc tggcctacca ggggtttgtg cgcgctttgc acgccgatct
    10621 ggctgcccac ggcttcgatg acgtggggaa gtctgacggc ctggtctttc gtctcctgag
    10681 cgatcgctcg cgcacggtga gtgagcttgc tgggctcctg gaggtgtcca agcagggggc
    10741 cgggcagatc atccaggaca tggagacgcg cggttacgtc gtgcgcgacc agagccccgg
    10801 cgatgctcgc gcccggccgg tgcgcctcag cgaccgtggc ttagttgcgc ttgccgcggc
    10861 tcggcggttt catcgccgca ccgaggctcg cctagttcgc gaacacggca gggaatcggt
    10921 ggctaccttc agagcggtgc tcacggcaat ggccggcgac gacgccacag ctttggatca
    10981 gcaggtccgg gggctctact tctaggggtt ccctgatcga ccgtcgcctg aagcccgcca
    11041 ctggcgcgag acgcaaaggg tcggctccca cacggggagc cgaccctttg acgtatgccg
    11101 cgtcgtcgcc
//
LOCUS       DAOMZY010000080        10929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5628_length_10929_cov_6.659371, whole genome shotgun sequence.
ACCESSION   DAOMZY010000080 DAOMZY010000000
VERSION     DAOMZY010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 10929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10929
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5628_length_10929_cov_6.659371"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PLK64_06215"
     CDS             <1..494
                     /locus_tag="PLK64_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HPV79371.1"
                     /translation="WLFICGEYPQAGPLSQQMKSQGLSVPLMGGDGIFDPKYIELAGA
                     TANTDLATSVGAPTESTEAGKKFLEQYKAAGYAEPAAAYGGYAYDAAKAIIEAAKTAL
                     KDAKDVKSARTAELTALGKVSFDGVTGKVAFDEFGDTTSKVLTVYKVDGGKWIAAKTD
                     TFK"
     gene            651..1574
                     /locus_tag="PLK64_06220"
     CDS             651..1574
                     /locus_tag="PLK64_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPV79372.1"
                     /translation="MDQFLQQLLNGVTLGSLYALIAVGYTVVYGIVQLINFAHGEVFM
                     IGAFGSLATWTLFFKGQTGLWVLPFMIIGAVLASVAVALLLERVAYRPLRNAPRLAPL
                     ITAIGISVFLQEAVRLFYPGAKSALPFPQIDLVTGAAFQVGGITVRRSAIFVIGALVV
                     CAVLLWFFINRTKMGRGMLAVSQDPDTARLMGINVDRIIAVAFAIGAALAGVAGVAQG
                     LQNSNIDFFIGFMAGLKAFTAAVLGGIGNIYGAVVGGLVLGVVEAMAVQFIPGTFGGS
                     PWKDVWAFVLLIVVLVFRPQGLMGARVVDRA"
     gene            1571..3280
                     /locus_tag="PLK64_06225"
     CDS             1571..3280
                     /locus_tag="PLK64_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPV79373.1"
                     /translation="MTTTQSRAAVSNPRPSSQWLLTLVGVVLLVVGCLLPWAFVPAIL
                     GNVTVLLYPGAMQILMIIVGAIALFFLLLRTGRFAAADRLLDSARGLRALSVTAAAFM
                     VLAVVIIAMQAPGLANADPGVYVSLVGALLMVAGSFRMPSDPKVDPITVHLPSWVEIV
                     VIVVMLAIALFGAAYALGQEDGTIFSLFLLFLAGSVVALARAGLFSWIGVIAQRNRRV
                     ITLGAFAVAFLFPFTQNGSDANMSIATQVLIFATTALGLNIVVGLAGLLDLGYIAFLG
                     AGAYVSAMMSHSAFATIGWKPPFIVVVAIAACVSSILGLIIGSPTLRVSGDYLAIVTL
                     AFGEIFRLTVNNLDGNNGPDLTNGPNGIPGIPELEIGSFNFGDTHTLLGIPLGRFANY
                     YFLMLVVAAIIITIFTRLNNSRIGRGWVAIREDEKAAEAMGVNVFGLKLFAFAGGAFL
                     AGMAGSIKAHQDVSVTPDQYIFLESAFLLAAIVLGGMGTVAGVLVGATVLKLLPEKLR
                     FVADYRLLTFGLLLVLMMRFRPEGLVPSRRRALEFHEDDTKLAERIEEAHLEGMTKTE
                     GRA"
     gene            3277..4125
                     /locus_tag="PLK64_06230"
     CDS             3277..4125
                     /locus_tag="PLK64_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV79374.1"
                     /translation="MTTTTHDRPSAPAASGKAVLEATGVIMRFGGLTAVNNVNLRVNE
                     GEIVGLIGPNGAGKTTFFNCLTGMYKPTEGEVKFQGTVLPPKPRAVVQAGMARTFQNI
                     RLFANMTALENVMVGRYCRTSAMALTSIIRGPKYRHEEEATRARAQELLNFVGLGKTS
                     EHLARNLPYGDQRRLEIARALATDPKLILLDEPTAGMNPKETEEAKELIFRIRDMGLA
                     VVVIEHDMRFIFTLCDRVLCLVRGETLIEGSPQVVQSDPRVIEAYIGKPDDDELEEEA
                     AKEDQA"
     gene            4131..4835
                     /locus_tag="PLK64_06235"
     CDS             4131..4835
                     /locus_tag="PLK64_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV79375.1"
                     /translation="MLEVKDLRVSYGKIEAVKGISFSVGQGEVVCLIGTNGAGKTTTL
                     RTISGLIRPTSGEITFEGKRLDGVPAHEIVTLGLAHSPEGRRIFPRLSVEENLLLGAF
                     SRNDGEIKSDLQAAYDLFPILGERSKQPAGTFSGGEQQMLAMGRAMMSRPKLLMLDEP
                     SMGLSPIMMQRIMSTVKTLQSQGTTILLVEQNARAALKLADKGYVLEVGHVVLEGSGH
                     DLLHSDAVRKAYLGED"
     gene            complement(4966..5562)
                     /locus_tag="PLK64_06240"
     CDS             complement(4966..5562)
                     /locus_tag="PLK64_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HPV79376.1"
                     /translation="MPEARRTVTGEDWYGREVQGERFADTDFVAVDFGDLRSVGAVLE
                     NCTFRRCRFNTSVHTDSAFTGCTFAYCTFFDARFVRCKLVGSTFTGCEFDLVQVDGGN
                     WSFVALPGAALGSTQWRGVRLTEADLDRVQAVGAVWRDLDLTGASLVGIDLRQADLRG
                     SSLWSLDPAAAQLSGAIVTPEQALVIAAALGLDVRVDD"
     gene            complement(5565..8567)
                     /gene="uvrA"
                     /locus_tag="PLK64_06245"
     CDS             complement(5565..8567)
                     /gene="uvrA"
                     /locus_tag="PLK64_06245"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPV79377.1"
                     /translation="MAERQPGRHSRDTLIVRGAREHNLKDISVELPRDSLIVFTGLSG
                     SGKSSLAFDTIFAEGQRRYVESLSAYARQFLGQMDKPDVDFIEGLSPAVSIDQKSTNR
                     NPRSTVGTITEVYDYLRLLFARVGRPHCPICDEPITRQSPQQIVDRLLELPERTRFQI
                     LAPVVKARKGEFVDLFAELQTKGFARARVDGEVIALADPPKLEKQIKHTIEVVIDRLV
                     AKGDDAAAKRRLTDSVETALVLAGGILLVDFVDREEGEPDKERRFSERMACPNDHPLA
                     IDEVEPRTFSFNSPFGACPACTGLGTELEVDPELCIPDEDLSIAQGAVAPWGSGSGSA
                     DYFMRVVQAVADDLKFSLDTPWRALPERAKEALLFGQNYKVHVTYRNRYGRDRSYTTG
                     FEGVVPFVKRRHSETDSEWSRDRYEGYMREVPCPVCRGARLKPEVLAIRVGGQNISQV
                     CALAINECAEFLRSVDFTDRERQIAERVIKEIGARLGFLLDVGLDYLSLDRPAGTLSG
                     GEAQRIRLATQIGSGLVGVLYVLDEPSIGLHQRDNHRLIETLTRLRDLGNTLIVVEHD
                     EDTIATADWVVDIGPGAGEHGGHVVHCGPVAGLLAHPDSLTGMYLSGRKSIPVPDVRR
                     PQVKGREVTVIAARENNLRDVTVSFPLANLVAVTGVSGSGKSTLVNDILYTVLANKLN
                     KARQVPGRHKSVTGLEHLDKVVHVDQSPIGRTPRSNPATYTGVFDHMRRLFAETTEAK
                     IRGYLPGRFSFNVKGGRCDNCSGDGTIKIEMNFLPDVYVPCEVCHGARYNRETLDVHF
                     KGKTIADVLDMPIEEAAEFFAAVPAIARHLTTLNDVGLGYVRLGQPAPTLSGGEAQRV
                     KLAAELQKRSSGRTVYVLDEPTTGLHFEDISKLLGVLQGLVDKGNTVIVIEHNLDVIK
                     SADWIVDLGPEGGSGGGRVVAEGTPEQVAAHPDSHTGRFLAPVLAKAAAGMPSARPSV
                     ERASARPRKATAVASGTAKRTVRGRHTE"
     gene            complement(8731..9468)
                     /locus_tag="PLK64_06250"
     CDS             complement(8731..9468)
                     /locus_tag="PLK64_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPV79378.1"
                     /translation="MGRSPVVSLVESQSEGWAVWRVRMLGDFVNGFAFRDANGAVTLL
                     DVGLPWSTRRVLAALAAIDSGPSDVARIMVTHAHSDHVGAAASLAARTGRAVDVHEAD
                     AAYVAAGESPPMSGLGGLVSRVVPGFAAAPVGVRLVDGQLLGVAGGLRVLHTPGHSPG
                     HVSFLHEPSRLLITGDAIFNIRGPRWPVKAFCTDFQLNRQTARLLGDLDYTMAAFTHG
                     PELRDSPREAIRDFLRREASGAVGGGT"
     gene            9541..10236
                     /locus_tag="PLK64_06255"
     CDS             9541..10236
                     /locus_tag="PLK64_06255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HPV79379.1"
                     /translation="MTTDTSWADVAAATAALLETAYGMREGDQAADSLCAGWTRGHVL
                     THVARNADAIGNLVTWAVTGVPTPMYASAEAREADIEAGAARPVADLVADVEASAARL
                     AGQTADLAPAHDAVEIELRGGWRIRASELAWRRLREVAYHHVDLDAGFGFADLPPDLT
                     SAFLADEVAGLRRHPQAPALTLHTTTGEEFTVGAGTAYVTGTPAALLGWLARGLTEGL
                     ATDGALPHPPKGT"
     gene            10238..10903
                     /locus_tag="PLK64_06260"
     CDS             10238..10903
                     /locus_tag="PLK64_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPV79380.1"
                     /translation="MSRYAGHVTSGGASDVRELRELIIRKCSVGEYDNNAYLLTCRTT
                     GEQLLVDAAADAPRLRAFVAEGTGNLGGIVTTHRHGDHVRALAEIAAWSGAPTAAGAE
                     DAAALPLAPDRALVHGDVLRVGAVALDVVHLRGHTEGGVALAYADPAGFTHLITGDSL
                     FPGGVGNTKMPGQSFEQLYADVTARIFDVYGDDTWVYPGHGDDTTLGVERPYLAEWLA
                     RGW"
BASE COUNT         1720 a         3808 c         3652 g         1749 t
ORIGIN      
        1 cctggctctt catctgcggc gagtacccgc aggccggtcc gctttcgcag cagatgaaga
       61 gccagggcct gtccgttccg ctcatgggtg gcgacggcat cttcgacccg aagtacatcg
      121 aactggccgg cgccacggcc aacactgacc tcgcgacgtc ggtgggtgct ccgaccgagt
      181 cgaccgaggc cggcaagaag ttcctcgagc agtacaaggc tgccggctac gccgagcccg
      241 ccgctgccta cggtggctac gcctacgacg ccgccaaggc catcatcgag gccgctaaga
      301 cggccctcaa ggacgccaag gatgtcaagt ccgctcgcac cgccgagctg accgcgctgg
      361 gcaaggtctc cttcgacggc gtgaccggca aggtcgcctt cgacgagttc ggtgacacga
      421 cctcgaaggt cctcacggtc tacaaggtcg acggtggcaa gtggatcgcc gccaagaccg
      481 acaccttcaa gtgatcggcc cgaaccgcgc atagcacgaa tagtcagaca cacaggggcg
      541 agcgctagcg ctcgcccctg tgtgctgcca cactctcgcc ttacgttcca cgtagggggg
      601 cctcggccaa tcaccatgca tcaacacccg tctcgtcctc aggaggtgac atggatcagt
      661 tcttgcaaca gctactcaac ggggtcaccc tcggatcgct gtatgcactg atcgctgtcg
      721 gctataccgt cgtctacgga atcgtccagc tcatcaactt cgcccacggc gaggtgttca
      781 tgatcggagc gttcggttcc ttagcgacat ggaccttgtt cttcaagggc cagaccgggc
      841 tgtgggtgtt gccgttcatg atcatcggcg ccgtcctcgc atcggtcgcc gtcgcgttgc
      901 tcctcgagcg ggtcgcctac cgaccgctgc ggaacgcccc ccgactggcg ccactcatca
      961 ccgccatcgg tatctcggtg ttcctccaag aggccgtgcg actgttctat cccggcgcga
     1021 agagcgccct gcccttcccg cagatcgacc tcgtgaccgg cgcggccttc caggtcggcg
     1081 ggatcaccgt ccgtcggtcg gcgatcttcg tcatcggtgc cctcgtcgtc tgcgccgtcc
     1141 tgctgtggtt cttcatcaac cgcaccaaga tgggccgcgg gatgctcgcc gtgtcccagg
     1201 accccgacac cgctcgcctc atggggatca acgtcgaccg gatcatcgcg gtcgcgttcg
     1261 ccatcggcgc cgcgcttgcc ggcgtggccg gcgtcgcgca gggtctgcag aacagcaaca
     1321 tcgacttctt catcggcttc atggccggac tcaaggcctt caccgcggcc gtcctcggcg
     1381 gcatcggcaa catctatggt gcggtcgtcg gcgggttggt cctcggcgtc gtcgaggcga
     1441 tggccgtcca gttcatcccc ggcacgttcg gtggatcccc gtggaaggac gtgtgggcct
     1501 tcgtgctcct catcgtcgtg ctcgtgttcc ggccccaagg cctcatgggc gcccgggtgg
     1561 tggatcgcgc atgacgacca cccagtcccg tgccgcggtg tccaacccgc gccccagcag
     1621 ccagtggctc ctcaccctcg tcggtgtggt gctgctcgtc gtcggctgct tgctgccctg
     1681 ggccttcgtc ccggccatct tgggcaacgt caccgtcctg ctctacccgg gcgccatgca
     1741 gatcctcatg atcatcgtcg gcgccatcgc cctgttcttc ctcctcctgc gcacgggccg
     1801 cttcgctgcc gctgaccggt tgttggacag cgcccgcggc ctgcgtgcgc tgtcggtgac
     1861 ggccgcggcc ttcatggtcc tcgcggtcgt catcatcgcc atgcaagcgc cggggctggc
     1921 caacgccgac cctggggtct acgtctcgct ggtgggcgca ctgctcatgg tcgccggcag
     1981 cttccggatg ccgtcggatc ccaaggtcga cccgatcacg gttcacctcc cgtcctgggt
     2041 ggagatcgtc gtcatcgtcg tcatgctcgc gatcgcgttg ttcggcgcgg catacgccct
     2101 cggtcaggag gacggcacga tcttctcgct gttcctgctg ttcctcgccg gcagcgtggt
     2161 cgccctcgcg cgcgccgggc tgttctcctg gatcggtgtc atcgcgcaac gcaaccgtcg
     2221 ggtcatcacc ctgggtgcct tcgccgtggc cttcctgttc ccgttcaccc agaacgggtc
     2281 cgacgcgaac atgtccatcg ccacccaggt gctcatcttc gcgacgaccg ccctcggcct
     2341 gaacatcgtc gtcggcctgg ccggcctgct cgacctcggc tacatcgcct tcctcggcgc
     2401 cggggcctac gtgtcggcga tgatgtcgca ctccgcgttc gcgacgatcg ggtggaaacc
     2461 accgttcatc gtcgtcgtcg ccatcgctgc ctgcgtgtcg tcgatcctcg gcctcatcat
     2521 cggctcacca actctgcggg tgtccggtga ctacctggcc atcgtcaccc tcgccttcgg
     2581 tgagatcttc cggctgaccg tcaacaacct cgacggcaac aacggcccgg acctcaccaa
     2641 cggcccgaac ggcatcccgg gcatcccaga gttggagatc gggtcgttca acttcggcga
     2701 cacgcacacc cttctgggca tcccgctcgg ccggttcgcc aactactact tcctcatgct
     2761 cgtcgtcgcg gcgatcatca tcacgatctt cacccggctc aacaacagcc ggatcgggcg
     2821 cggctgggtc gcgatccgtg aggacgagaa ggccgccgag gccatgggcg tcaacgtctt
     2881 cgggctcaag ctgttcgcct tcgccggcgg tgcgttcctc gccggcatgg ccgggtcgat
     2941 caaggcgcac caggacgtgt cggtcacccc cgaccagtac atcttccttg agtccgcctt
     3001 cctgctcgcc gcgatcgtcc tcggcggcat ggggacggtg gccggcgtcc tcgtcggtgc
     3061 caccgtgctc aagttgctcc ccgagaagct gcgcttcgtg gccgactacc ggctgctcac
     3121 cttcggtttg ctcctcgtgc tcatgatgcg gttccgcccg gagggcttgg ttccgagtcg
     3181 gcgccgcgcc ttggagttcc atgaggacga caccaagttg gccgaacgaa tagaagaagc
     3241 ccatctggaa ggtatgacga agacggaggg acgggcatga ccaccaccac gcacgaccgt
     3301 cccagcgccc ccgcggcgtc cggcaaggcc gtccttgagg cgaccggcgt catcatgcgt
     3361 ttcggcggcc tcacggccgt caacaacgtc aacctccggg tcaacgaggg cgagatcgtc
     3421 ggcctcatcg ggcccaacgg tgccgggaag acgacgttct tcaactgcct caccggcatg
     3481 tacaagccca ccgagggcga ggtgaagttc cagggcacgg tattgccacc caagccacgg
     3541 gccgtggtcc aggcggggat ggctcgcacc ttccagaaca tccgcctctt cgccaacatg
     3601 acggcgttgg agaacgtcat ggtcggtcgc tactgccgaa ccagcgccat ggccctcacc
     3661 tcgatcatcc gtggcccgaa gtaccgccac gaggaggagg ccacccgggc tcgcgcccag
     3721 gagttgctca acttcgtcgg tttgggcaag accagcgaac acctcgctcg caacctcccc
     3781 tacggcgacc agcgtcgcct cgagatcgcg cgggcgctgg ccaccgaccc gaagctcatc
     3841 ctcctggatg agccgacggc cggcatgaac cccaaggaga ccgaagaggc caaggagttg
     3901 atcttccgga tccgcgacat ggggctggcc gtcgtcgtca tcgagcacga catgcggttc
     3961 atcttcaccc tgtgcgaccg ggtgctctgc ctcgtccgtg gcgagaccct catcgagggc
     4021 agcccgcagg tcgtccagag cgatccacgg gtcatcgagg cgtacatcgg caaacccgat
     4081 gacgacgagc tcgaggaaga ggccgcaaag gaggaccagg cgtgagcgcc atgctcgagg
     4141 tcaaggacct tcgggtctcc tacggcaaga tcgaggccgt caagggaatc agtttcagcg
     4201 tcggtcaggg cgaggtggtc tgcctcatcg ggaccaacgg tgccggcaag acgacgacgc
     4261 tgcgaacgat ctccggtctc atcaggccca catcgggtga gatcaccttc gagggcaagc
     4321 gtctggacgg cgtcccggcc cacgagattg tcaccctggg cctggcccac agcccggagg
     4381 gtcggcggat cttcccccgg ctctcggtcg aggagaacct cctgctgggc gccttctcgc
     4441 gcaacgacgg cgagatcaag agcgacctgc aggcggccta cgacctgttc ccgatcctcg
     4501 gagagcggtc caagcaacct gccgggacgt tctccggtgg cgagcagcag atgctcgcca
     4561 tgggccgggc gatgatgagc cgtcccaagc tcctcatgct ggacgagccg tccatgggtc
     4621 tgtcaccgat catgatgcag cgcatcatgt cgaccgtgaa gaccctgcag agccagggca
     4681 cgacgatcct gctcgttgag cagaacgcgc gcgctgcact caagttggct gacaaggggt
     4741 acgtgctcga ggtcggccac gtcgtcctcg aaggctccgg acacgacctg ctgcacagtg
     4801 acgccgtccg taaggcgtac ctcggcgagg actagcgctc acggcacgtc agcacagcac
     4861 agcgcaacgc aactcaacac agcaagacca gcacggtatg ccggtggggc ggggccaggt
     4921 ggccccgccc caccgtcgtg cgtttcgggc cgcgcccgcc aaccctcagt cgtcgacgcg
     4981 aacgtccagc ccgagtgcgg cggcgatgac gagcgcctgc tcaggcgtca cgatcgctcc
     5041 actcagctgt gcggcagccg gatcgagcga ccagagcgaa cttccgcgca agtctgcctg
     5101 acgcaggtcg atcccgacca gcgaggcacc cgtgaggtcg aggtcgcgcc acacggcgcc
     5161 gaccgcctgc acccggtcga ggtcggcctc ggtgaggcgg acaccgcgcc actgggtgga
     5221 cccgagggcc gcgcccggca gagccacgaa actccagttg cccccgtcga cctggacgag
     5281 gtcgaactcg cacccggtga aggtcgatcc gaccagtttg cagcggacga accgggcgtc
     5341 gaagaaggtg cagtaggcga acgtgcagcc ggtgaaggcc gagtcggtgt gcaccgaggt
     5401 gttgaaccgg caccgccgga acgtgcagtt ctcgaggaca gccccgacgc tccgcagatc
     5461 gccgaaatcg accgccacga agtccgtgtc ggcgaaccgt tcaccctgca cctcgcggcc
     5521 gtaccagtcc tcccccgtga ccgtccggcg cgcctcgggc acggtcattc ggtatgccgc
     5581 ccgcgcaccg tccgcttcgc cgttccgctg gccaccgccg tggccttgcg gggtcgggca
     5641 ctggcccgct cgacgctggg gcgcgcggac ggcatacccg ctgcggcctt ggcgagcacg
     5701 ggggcgagga agcgacccgt gtgggagtcc ggatgcgcgg cgacctgctc gggggtgcct
     5761 tcggcgacga cgcggccgcc gccggagccg ccctcgggac cgaggtcgac gatccagtcg
     5821 gccgacttga tgacgtcgag gttgtgctcg atgacgatga ccgtgttgcc cttgtcgacc
     5881 aggccctgca gcacgccgag gagtttgctg atgtcctcga agtgcaggcc ggtggtcggc
     5941 tcgtcgagga catagacggt gcggccgctg gagcgcttct gcaactcggc ggcgagcttg
     6001 acccgctggg cctcaccacc cgagagcgtc ggcgcgggtt ggccgagccg gacatagccc
     6061 agcccgacat cgttcaacgt cgtcaggtgg cgagcgatcg ccggcactgc cgcgaagaac
     6121 tcggcggcct cctcgatggg catgtcgagg acgtcagcga tcgtcttgcc cttgaagtgg
     6181 acgtcgaggg tctcccggtt gtagcgcgcc ccgtgacaga cctcgcacgg cacatagaca
     6241 tccggcagga agttcatctc gatcttgatc gtgccgtcgc ccgagcagtt gtcgcagcgc
     6301 ccgcccttga cgttgaacga gaaccggccc ggcaggtagc cacgaatctt cgcctcggtg
     6361 gtctcggcga acaggcggcg catgtggtcg aagactccgg tgtaggtggc cgggttggac
     6421 cgcggcgtgc ggccgatcgg gctctggtcg acgtggacga ccttgtcgag atgctcaaga
     6481 ccggtgaccg acttgtgccg ccccgggacc tggcgggcct tgttgagctt gttggccaac
     6541 accgtgtaga ggatgtcgtt gaccagcgtc gacttccccg agccggacac cccggtgacc
     6601 gccacgaggt tggccagggg gaacgacacg gtgacgtcgc gcaggttgtt ttcgcgggcg
     6661 gcgatcaccg tcacctcgcg gcccttgacc tgcgggcgac gcacgtcggg gaccgggatc
     6721 gacttgcgac ccgagagata catcccggtg agcgagtcgg gatgagccag cagcccggcg
     6781 accggcccgc agtgcacgac atggccgccg tgctcccccg ctcccggccc gatgtcgacg
     6841 acccagtcgg ccgtcgcgat ggtgtcctcg tcgtgctcga cgacgatgag ggtgttgccg
     6901 aggtcgcgca gccgggtcag ggtctcgatg agccggtggt tgtcgcgctg atgcagcccg
     6961 atcgagggct cgtccagcac gtacagaacg ccgaccaggc cggacccgat ctgggtggcc
     7021 aggcggatcc gctgggcctc gccgccggac agcgtgccgg cggggcgatc cagcgacaag
     7081 tagtcgaggc cgacgtcaag caggaacccc aaccgggccc cgatctcctt gatgacccgc
     7141 tcggcgatct gccgctcgcg gtcggtgaag tcgaccgagc gcaggaactc ggcgcactcg
     7201 ttgatcgcca gggcacagac ctgcgagatg ttctgcccac ccacccggat ggcgagcact
     7261 tccggcttga gccgggcacc gcggcatacc gggcagggca cttcacgcat gtagccctcg
     7321 tagcggtccc ggctccactc ggagtcggtc tcggagtggc gacgcttgac gaacggcacg
     7381 accccttcga aaccggtggt gtaggaccgg tcgcggccgt agcggttgcg atacgtgacg
     7441 tggaccttgt agttctgccc gaagagcagg gcctccttgg cccgttcggg gagcgcccgc
     7501 cagggggtgt cgagcgagaa cttcaggtcg tcggccaccg cctggacgac gcgcatgaag
     7561 tagtcggccg agccggagcc gctcccccac ggagccaccg cgccctgcgc gatcgagagg
     7621 tcctcgtcgg ggatgcacaa ctcggggtcg acctcgagct cggtgcccag accggtgcag
     7681 gccgggcagg cgccgaaggg cgagttgaac gagaaggtcc ggggctcgac ctcgtcgatg
     7741 gccaaggggt ggtcgttggg gcaggccatc cgctcggaga agcgccgctc cttgtcgggc
     7801 tcgccctcct cgcggtccac gaagtcgacg aggagtatgc cgcccgccag cacgagggcg
     7861 gtctccaccg agtcggtgag ccgtcgcttg gccgccgcgt cgtcaccctt ggcgaccaac
     7921 cggtcgatga ccacctcgat ggtgtgcttg atctgcttct cgagcttggg cgggtcggcc
     7981 agcgcgatga cctcaccgtc gaccctggcc cgggcgaagc ccttggtctg caactcggcg
     8041 aacaggtcga cgaactcgcc cttacgggcc ttgacgaccg gcgcgaggat ctggaagcgg
     8101 gtgcgctcag ggagctcgag cagtcggtcg acgatctgct gggggctctg gcgggtgatc
     8161 ggctcgtcgc agatggggca gtgcgggcga ccgacccggg cgaacaacag acggaggtag
     8221 tcgtagacct cggtgatcgt ccccaccgtc gagcgtgggt tgcggttggt cgacttctgg
     8281 tcgatcgaca cggccgggga gaggccctca atgaagtcga cgtcgggctt gtccatctgg
     8341 ccgaggaact ggcgggcata ggccgacaag gactcgacgt aacgacgctg cccctcggcg
     8401 aagatcgtgt cgaacgccag cgacgacttc cccgagccgg acaggcccgt gaagacgatg
     8461 aggctgtcgc ggggcaactc gaccgagatg tccttgaggt tgtgctcgcg ggccccccga
     8521 acgatcaggg tgtcgcggct gtgtcggccc ggctgtcgtt ccgccacgag atgcgcgaca
     8581 gcagccccgc cggccgtcgc cgggtccggc ttggtggacg acgatgtgga cgacttggtg
     8641 gacggcaccg ccgaaggtgc ggcggatcgc ggggtggatc cggagcgttt ccgggtgggc
     8701 gaagggggca cgactgacac gcaggacagc ctaggtgccc ccaccgacag cccccgacgc
     8761 ttcccggcgg aggaagtccc ggatcgcctc ccgggggctg tcgcgcagct ccggcccatg
     8821 ggtgaaggcc gccatcgtgt agtcgaggtc gcccaggagt ctggcggtct gccgattgag
     8881 ttggaagtcg gtgcagaacg ccttgaccgg ccaacgcggc ccacggatgt tgaagatggc
     8941 atcgcccgtg atgagcaacc gactcggctc gtgcaggaac gacacatgcc ccggcgaatg
     9001 gccaggagtg tgcaggaccc gcaggccgcc ggctacgccg agcagctggc cgtcgaccag
     9061 ccggaccccc accggcgcgg cggcgaagcc cggcacgacc cgcgacacga gcccgcccag
     9121 cccagacatc gggggtgact cccccgccgc gacgtatgcc gcgtccgcct cgtgaacgtc
     9181 cacggcccgg ccggtgcgag cggccagcga ggccgccgcg cccacatgat cggagtgcgc
     9241 gtgggtgacc atgatccggg cgacgtccga cggaccggag tcgatcgcgg ccagagccgc
     9301 caggacccgc ctcgtcgacc acgggaggcc gacatcgagc agggtcactg caccgttcgc
     9361 gtcgcgaaag gcgaacccgt tgacgaagtc gccgagcatc cgcacccgcc acaccgccca
     9421 gccctcggac tgcgactcga ccagggacac gaccggggaa cgacccatgc cacacaccat
     9481 aggccgacgc cgccgcgccg cgacagcgcc ggacccggat ccgccgggta gcgtcaggcc
     9541 atgaccaccg acacctcatg ggccgacgtc gccgcggcga ccgcggccct gttggagacg
     9601 gcatacggga tgcgtgaggg tgaccaggcg gccgactcac tgtgcgcggg gtggacgcgt
     9661 ggccacgtgc tcacccacgt ggcccgcaac gccgacgcca tcggcaacct cgtcacctgg
     9721 gccgtgaccg gcgtgcccac cccgatgtat gcgtccgctg aagcccgcga ggcggacatc
     9781 gaggccggtg cagctcggcc cgtggccgac ctggtcgccg atgtcgaggc gagcgcggcc
     9841 cggctcgccg gccagaccgc ggacctcgcc ccagcccacg acgcggtcga gatcgagttg
     9901 cgcggcggct ggcggatccg tgcgtcggag ctggcctggc gccggctgcg cgaggtggcc
     9961 taccaccacg tggatctcga tgccgggttc gggttcgccg acctgccacc agacctcacc
    10021 tcggccttcc tcgccgacga ggtggcgggg ttgcggcggc acccgcaggc gccggcgctc
    10081 accctgcaca cgacgacggg cgaggagttc acggtcgggg ccgggacggc atacgtcact
    10141 ggcacccccg ccgcgctgct cgggtggctg gcgcgcgggc tgaccgaggg gctcgccacg
    10201 gacggcgcgc tcccccaccc tccgaaaggg acctgagatg agcaggtatg ccgggcacgt
    10261 cacctccgga ggcgcgtcgg acgtccgcga gctgcgcgag ttgatcatcc gcaagtgcag
    10321 cgtcggcgag tacgacaaca acgcctacct gctcacctgt cggaccaccg gcgagcagtt
    10381 gctcgtcgac gcggcggccg acgcgccccg gctgcgggcc ttcgtcgccg agggcaccgg
    10441 caacctcggc gggatcgtca ccacgcaccg ccacggtgac cacgtccgcg cacttgcgga
    10501 gattgcggcc tggtcgggcg cccccacggc cgcgggggcc gaggacgccg ctgccctgcc
    10561 gctggctcct gatcgggcgc tcgtccacgg agacgtgctg cgggtcgggg cggtcgccct
    10621 cgacgtcgtc cacctgcgcg ggcacaccga gggcggggtc gcgttggcgt atgccgaccc
    10681 ggcgggcttc acacacctca tcaccggcga ctcgctcttc cccggcgggg tcggcaacac
    10741 caagatgccg gggcagtcgt tcgagcagtt gtatgccgat gtgacggcgc ggatcttcga
    10801 cgtctacggc gacgacacgt gggtctatcc gggccacggc gacgacacga ccctgggcgt
    10861 cgagcgcccg tacctcgccg agtggctcgc ccgcggctgg tgaggcgtta gccacgccct
    10921 ccgcacgcc
//
LOCUS       DAOMZY010000081        10928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5629_length_10928_cov_5.875011, whole genome shotgun sequence.
ACCESSION   DAOMZY010000081 DAOMZY010000000
VERSION     DAOMZY010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 10928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10928
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5629_length_10928_cov_5.875011"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..1105
                     /gene="argC"
                     /locus_tag="PLK64_06265"
     CDS             59..1105
                     /gene="argC"
                     /locus_tag="PLK64_06265"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012750950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HPV79381.1"
                     /translation="MMWTAAVAGASGYAGGEILRLLLGHPQIQIGAVTADSSAGKRLG
                     EVHPNLVPLGERVIEPTNPDTLAGHDLVFLALPHGASAALAAQLPDGVIVLDCGADFR
                     LVDEAAWTTFYDTPYAGQWPYGLPELPGVAGAKARTALTGATRIAVPGCYPTACSLAL
                     APGFTAGLLDPTDVVIVAASGTSGAGKSLKPHLLSAEVMGAMSPYGVGGVHRHTPEIE
                     QNLTAAAGSPVTVSFTPTLAPMSRGILATCTARLLPGADPAAVRAAWEAAYADEAFVH
                     LLASGVWPRTADVLGSNSVHIQVTVDERVGRVIAVAAVDNLAKGTAGGAIQCANIALG
                     LPETTGLPVAGVAP"
     gene            1102..2259
                     /gene="argJ"
                     /locus_tag="PLK64_06270"
     CDS             1102..2259
                     /gene="argJ"
                     /locus_tag="PLK64_06270"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007272475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HPV79382.1"
                     /translation="MTSTVNLPQGFRAAGVTAGLKASGRSDLALVVNDGPDHHAAAVF
                     TSNRVEAAPVTWSRQVVSDGRVDAVILNSGGANACTGSPGFLDTHRTAEKVGEVLGVS
                     AGDVVVCSTGLIGERLPMDLLLPGVDAVATALSVDGHNAAATAIMTTDTVSKQATFVG
                     DGWSVSGMAKGAGMLAPALATMLVVITTDAVTHPKEMDLVLRGATAVSFDRIDSDGCM
                     STNDTVLLMASGASGVKPGWQALLEGVTRVCLDLAVQLIADAEGAHHDIEVEVRSAAS
                     EGDALEVARAIARNNLFKCAVFGNDPNWGRVLAAVGTTRAAFDPARLAVTMNGVQVCR
                     DGGVGEDRSLVDLTPRHTSVIVDLHEGEAHATVWTNDLTHDYVHENSAYST"
     gene            2277..3212
                     /gene="argB"
                     /locus_tag="PLK64_06275"
     CDS             2277..3212
                     /gene="argB"
                     /locus_tag="PLK64_06275"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HPV79383.1"
                     /translation="MMSSLRGVIDAAAVRVAASKAATLVEALPWLERFRGALVVVKYG
                     GNAMTDDTLKAAFAQDIAFLRYAGLHPVVVHGGGPQIGAMLKRMGLTSEFKGGLRVTT
                     PEVMEIVRMVLTGQVQRELVGLINQHGALAVGLSGEDAHLFEARKRGTVVDGEEVDLG
                     LVGDVVGVNPAAVLDIIAAGRIPVVSTVAPDLDEPGQVLNVNADTAAAALAVALEARK
                     LVVLTDVEGIYADWPNRDSLLSQVTLSEARLLLERVDSGMVPKLEACIRAVEQGVPQA
                     HVIDGRAAHSVLLEVFTDEGIGTMVLPDPTGEMWA"
     gene            3209..4462
                     /locus_tag="PLK64_06280"
     CDS             3209..4462
                     /locus_tag="PLK64_06280"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018779115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HPV79384.1"
                     /translation="MNDLAGGSARGDASGTSHTGEWLGRYAASLIPVFGVPQTVLSHG
                     QGCYVVDVDGKRYLDLLAGIAVNTLGHGHPALVDAVTKQASQLVHVSNFFTSQPQVEL
                     AEALLRVAEAPAGSGVFFANSGTEAIEAAVKLSRRTGRGTIIAAEGAFHGRSTGALAL
                     TWKLAYREPFAPLIGRVVHVPFNDEAALAAAVAEIGDDLAAVILEPIQGEAGVNLSST
                     AYLQLARELTTAAGALLILDEIQSGMGRTGRWFAYQSRGVVPDAITLAKGLAGGVPIG
                     ALVTFGPEVTGLLSAGQHGSTFGGNPLACAAGVAVVDTIESDGLLDHAVEMGAHLADG
                     VAALGHPVIAGVRGEGLLRAIAFTEPVAAQVITLAREAGFIVNAVAPDALRLAPPLVI
                     EAHELDTFVAALPGLLDVALAGDPP"
     gene            4459..4935
                     /gene="argR"
                     /locus_tag="PLK64_06285"
     CDS             4459..4935
                     /gene="argR"
                     /locus_tag="PLK64_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018135602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HPV79385.1"
                     /translation="MIPATPTARRAKIAGLLADRSVRSQGELLTLLADAGIEVTQATL
                     SRDLEALGAVKVRAGRGLVYAVPGASPSPVEQVPSRLRRLLEELLVTADASGNLVVLR
                     TPPGAANHLASTIDASHLTDVLGTIAGDDTILLVTSSAKGGRAVATRLLALAGASA"
     gene            4999..6456
                     /gene="argH"
                     /locus_tag="PLK64_06290"
     CDS             4999..6456
                     /gene="argH"
                     /locus_tag="PLK64_06290"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HPV79386.1"
                     /translation="MNTERPGMPHEDSAGTSARVSLWGGRFAGGPADALAELSRSTHF
                     DWRLAAYDIAGSQAHARVLHRAGLLSDGDLATMLGALDELLVDVHTGAFVPSPEDEDV
                     HTALERGLIERAGADVGGRLRAGRSRNDQIATLLRMCLRDAARDLAAQVLDLVDALVG
                     QAQSHLGVAMPGRTHMQHAQPVLLSHHLLAHAWALLRDVDRLRDWDRRADSSPYGSGA
                     LAGSSLGLDPEAVARDLGFSRSVENSIDGTASRDVAAEFAFVMAMTAVDVSRIAEEIV
                     VWATKEFGYVTLDDAYSTGSSIMPQKKNPDIAELARGKAGRVVGDLTGLLTTLKGLPL
                     AYNRDLQEDKEPVFDAIDTLAVLLPAFTGMVQTLVFNRERLESLAPQGFALATDIAEW
                     LVRQGVPFRVAHEVAGACVRECEGRGIELWELTDAELAAVNPALTPAVREVLSVAGSL
                     ASRDARGGTAPVRVGEQLAEAQAAVASARAFAAPA"
     gene            complement(6651..7607)
                     /locus_tag="PLK64_06295"
     CDS             complement(6651..7607)
                     /locus_tag="PLK64_06295"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HPV79387.1"
                     /translation="MPSTATTGQVTLRFLAAPTDAGQGGSVSGGRILEWIDKAGFACA
                     AGWSGRYCVTAYVGNIDFHRPVQVGDLVEVSAQLVLTGRSSMTIVVDVSSGNPRGGQV
                     EHAFECRMVFVAVDDEGRTVDVAQWDPPGAVLRAAQAEARRRIDLRERVKSLVAAQDW
                     EGATLAHGATLRFLAAPTDVNWGGKVHGGILMRWLDETGYVCASGWSQTHVVSRFSGG
                     VSFLRPIRIGDVVEIQSRLVHTGRTSMHVALRVLAGSPRTGELALATQCTSVYVALDA
                     DGRPCPVPTWTPTAAVDLSAQERALEFMALRADPAFHSSPRN"
     gene            7647..8264
                     /locus_tag="PLK64_06300"
     CDS             7647..8264
                     /locus_tag="PLK64_06300"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HPV79388.1"
                     /translation="MSRLPSGFYDRPVLDVAVDLLGARISHGGVTIRLTEVEAYAGVG
                     DPGSHAFRGETPRTTVMFGAPGGLYVYFTYGMHWCANLVCGPDGQASAVLMRAGEVVD
                     GEEIARTRRGEACATRDLARGPARLASALGLTGEVNGTLTFEPDSPVRVQAAAEAPDP
                     ARIRTGPRVGVSGTGGDGTAYPWRFWIDGEPTVSAYRPGKPRRRP"
     gene            complement(8283..9203)
                     /locus_tag="PLK64_06305"
     CDS             complement(8283..9203)
                     /locus_tag="PLK64_06305"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HPV79389.1"
                     /translation="MEPLHDDEADTGLEVVLDLLRSQAPHLAALSLARISGTDGNTGS
                     DNVLYRLGTELVVRLPRTPSAATSLRTETRLLPHLAGRLPVALPELVHLGSASARYPH
                     PWAVLRWLPGRDAWADRATLDDPHGFRLAEDLAAVVDALGKVPRAAYGDIVEVRAPGR
                     RGGGLSPLTEDIDEWLGEAHGLVDERGVRASWQESLSAREWDGDAVLCHGDLIPGNLL
                     VQGNRLTAVIDWGGAGAGDPALDLVPAWAVLGPQGRERFRDGAAADDDSWLRARGYAL
                     QQAVAGVIYYTPRRHPLADVMRRTLHAILR"
     gene            9332..9922
                     /locus_tag="PLK64_06310"
     CDS             9332..9922
                     /locus_tag="PLK64_06310"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HPV79390.1"
                     /translation="MRALAIRLGAQPWLPRLAPVVLGLDKALFAATHGRAGVLSMSGL
                     PWITLTVVGRRTGLVRRVPLLAVPHDDGWLVAGSNWGGPATPAWVANLTAAGRAVVTR
                     SGRDTPMSARRVTGSERAQLWAEMLATWPNFERYAQRTTREIPVFVLTQVGPTVAHVP
                     ARARHTGGRYLFARGGRHTAGLTQPDSTRTEGNPPP"
     gene            9919..>10928
                     /gene="tyrS"
                     /locus_tag="PLK64_06315"
     CDS             9919..>10928
                     /gene="tyrS"
                     /locus_tag="PLK64_06315"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPV79391.1"
                     /translation="MSDILDELQWRGSVAQTTDETALREALASGPITAYCGFDPTAPS
                     LHFGNLVQLVLLRRLQRAGHRVICLVGGSTGLVGDPRPTAERVLKTKEEAAANVARIK
                     ALVRPFLDFDGTDPAAAHPAILVDNLDWTAPMSALDFLRDIGKHFRVNQMIRKESVAR
                     RLESQEGISYTEFSYQLLQALDYLELYRVHGCTLQTGGSDQWGNLTAGVDLIHRAEGV
                     SVHALATPLLTDSSGSKFGKSEGNAVWLSADMTSPYAFYQYFLNVEDASVISLLKVFS
                     DLGADAIAELETAVAAEPFRRAAQRTLAAEITTLVHSSAATAAAQAASEAIFGKGDLA
                     AL"
BASE COUNT         1584 a         3874 c         3801 g         1669 t
ORIGIN      
        1 gcgtccacgt ggcgggacgg gtcgacgcat accttctcgc cgctctgtat gatcatgcat
       61 catgtggacg gcagcagtgg cgggtgcgag cgggtatgcc ggaggcgaga tcctccggct
      121 gctccttggc cacccccaga tccagatcgg tgcagtcacg gcggattcct cagccggcaa
      181 acgactcggt gaggtgcacc ccaacctcgt cccgctgggg gagcgcgtca tcgagccgac
      241 gaaccccgac acgttggccg gccacgacct ggtcttcctc gccctgcccc acggcgcctc
      301 cgccgcgctc gcggcgcaac tgcccgacgg cgtcatcgtc ctggactgcg gggccgactt
      361 ccggctcgtc gacgaggccg cgtggacgac cttctacgac accccgtatg ccggccagtg
      421 gccttacggt ctccccgagc ttcccggggt ggccggggcc aaagcccgca ccgccctgac
      481 cggagccacc cggatcgccg tgccgggctg ctacccgacc gcctgctcgc tggctctcgc
      541 ccccggcttc accgccggac tcctggaccc gaccgacgtc gtcatcgtcg ccgccagcgg
      601 cacctcaggc gctggtaagt ccctcaagcc acacctgctc agcgccgagg tgatgggcgc
      661 gatgtcgccc tacggcgtgg ggggagtgca ccggcatacc ccggagattg agcagaacct
      721 caccgcagcc gcgggcagcc cggtgaccgt gtcgttcacc cccaccctcg cgcccatgtc
      781 ccgcgggatc ctcgcgacgt gcaccgcgcg actgcttccc ggcgcggacc cggcggccgt
      841 tcgcgcggcg tgggaggcgg catacgccga cgaggccttc gtgcacctgc tggcgtcggg
      901 cgtctggccg cggacagccg atgtcctcgg cagcaacagc gtccacatcc aggtgaccgt
      961 cgatgaacgc gtcggccggg tcatcgcggt cgccgcggtc gacaacctcg ccaagggcac
     1021 ggccggcggc gcgatccagt gcgccaacat cgccctcggc ctccccgaaa ccaccggact
     1081 tcccgttgca ggagtggcac cgtgacctcg accgtcaacc ttccccaggg cttccgggcc
     1141 gccggcgtga cggccgggct caaggcgagc ggccggtcag acctcgccct cgtcgttaac
     1201 gacggccccg accaccatgc cgccgccgtg ttcacgagca accgggtcga ggcggcaccg
     1261 gtgacctgga gccgacaggt cgtcagcgac ggccgggtcg acgcggtgat cctcaattca
     1321 ggaggggcca acgcctgcac gggctcgccc ggcttcctcg acacccatcg cacggccgag
     1381 aaggtcggcg aggtcctcgg cgtttccgcc ggcgacgtcg tggtctgctc gaccggtctc
     1441 atcggtgagc ggctgccgat ggatctgctc ctccccgggg tcgacgcggt cgccacggcc
     1501 ctgagcgtcg acgggcacaa cgccgcggcg accgcgatca tgacgaccga caccgtctcc
     1561 aagcaggcga ccttcgtcgg cgacggctgg tcggtgagcg gcatggccaa gggcgccggc
     1621 atgctcgcgc ctgctctcgc aacgatgctc gtcgtcatca cgacagacgc cgtgacccac
     1681 ccgaaggaga tggacctcgt cctgcgcggg gcgacggccg tgtcattcga ccggatcgac
     1741 tccgacggct gcatgtcgac caacgacacc gtgctgctca tggcgtcggg cgcttccggg
     1801 gtgaagcccg gttggcaggc gctgctcgaa ggcgtcacgc gggtctgcct cgacttggcc
     1861 gtgcaactca tcgccgatgc cgagggcgcc caccacgaca tcgaggtcga ggtgcggtcg
     1921 gcggcctcgg agggcgatgc gcttgaggtc gcccgcgcga tcgcccgcaa caacctcttc
     1981 aagtgtgcgg tcttcggcaa cgaccccaac tggggtcggg tgctcgctgc ggtcggtacc
     2041 acgcgcgccg ccttcgaccc agcccgcctc gcggtgacga tgaacggcgt ccaggtatgc
     2101 cgcgacggcg gcgtggggga ggaccgctcg ctggtcgacc tgacgccacg gcatacctcc
     2161 gtgatcgtcg acctgcacga aggggaggcg cacgcgacgg tgtggaccaa cgacctcacc
     2221 cacgactacg tccacgagaa ctccgcctac agcacctgat gtcgaaggac ccaccgatga
     2281 tgagctccct gcgtggtgtc atcgacgccg ccgccgtgcg cgtcgccgca agcaaggccg
     2341 ccaccctcgt cgaagccctc ccgtggctgg aacgcttccg cggggcgctg gtcgtcgtga
     2401 agtacggcgg caacgcgatg actgacgaca ccctcaaggc ggccttcgcc caggacatcg
     2461 ccttcctccg gtatgccggc ctgcatccgg tcgtcgtcca cggtggcggc ccgcagatcg
     2521 gggcgatgct caagcggatg ggcctgacga gtgagttcaa gggcggcctg cgggtgacca
     2581 cccccgaggt catggagatc gtgcgcatgg tgctcaccgg ccaggtgcag cgcgagctcg
     2641 tcggcctgat caaccagcac ggcgcgctcg cggtcgggct gtccggcgag gacgcccacc
     2701 tgttcgaggc gcgcaagcgc ggcactgtcg tcgacggcga ggaggtcgac ctcggcctcg
     2761 tcggcgacgt cgtgggggtc aaccccgctg cggtgctcga catcatcgcg gccgggcgga
     2821 tcccggtggt ctcgaccgtc gcgcccgacc tcgacgagcc gggccaggtc ctcaacgtca
     2881 acgccgacac ggcggcggcg gcgctcgcgg tggcactgga ggcccgcaaa ctcgtcgtgc
     2941 tcaccgacgt cgagggcatc tatgccgact ggcccaaccg tgactcgctg ctctcgcagg
     3001 tcaccctctc ggaggcccgg ctgcttctcg agcgcgtgga ctcggggatg gtgcccaagc
     3061 tcgaggcctg catccgggcg gtcgagcaag gcgtgccgca ggcgcacgtc atcgacggcc
     3121 gcgccgcgca ctcggtgctg ttggaggtct tcaccgacga gggcatcggc acgatggtcc
     3181 ttcccgatcc gaccggagag atgtgggcat gaacgacctg gctggcggga gcgccagggg
     3241 cgacgcgagc ggcacctccc acacgggcga gtggctcggc cggtatgccg cctcgctcat
     3301 ccccgtcttc ggcgtcccgc agacggtcct ttcgcacggg caggggtgct acgtcgtcga
     3361 cgtcgacggc aagcggtatc tcgacctgct cgccgggatc gccgtcaaca ccctcgggca
     3421 cgggcatccg gctctcgtcg atgccgtgac aaagcaggca tcccaactag tccatgtctc
     3481 aaacttcttc accagccagc cgcaggtcga gctggccgag gcgctgcttc gggtcgccga
     3541 ggccccggcg ggctcgggtg tcttcttcgc aaactccgga accgaggcga tcgaggcagc
     3601 cgtcaagctg tcgcggcgca ccggccgagg aacgatcatc gctgccgagg gcgccttcca
     3661 cggccgctcg accggggccc tcgccctgac ctggaaactc gcctatcgcg agccgttcgc
     3721 gccgctcatc ggtcgcgtgg tgcatgtgcc gttcaacgac gaggctgccc tggcggccgc
     3781 ggtcgccgag atcggcgacg acctggcggc ggtgatcctc gagccgatcc aaggtgaggc
     3841 gggagtcaac ctcagcagca cggcatacct gcagctggcc cgcgaattga ccacggcggc
     3901 aggcgcgttg ctcatcctcg acgagatcca aagtgggatg ggtcgcaccg gtcggtggtt
     3961 cgcctaccag tcgcgggggg ttgtccccga cgcgatcacc ctcgccaagg ggctggccgg
     4021 gggagtgccg atcggggcgc tggtgacgtt cggacccgag gtgaccgggc tgctgtccgc
     4081 gggtcagcat gggagcacct tcgggggcaa cccgctcgcc tgcgcggccg gagtggcggt
     4141 ggtggacacg atcgagagcg acggcctgct cgatcacgcc gtcgagatgg gcgcgcacct
     4201 cgctgacggc gtcgctgccc ttggccaccc ggtgatcgcg ggagtgcgcg gcgagggcct
     4261 gctgcgggcc atcgccttca ccgaacccgt tgcggcacag gtgataacgc tcgcgcgcga
     4321 ggccgggttc atcgtcaacg cggtggcgcc cgacgcgctc cggttggccc ctccgctggt
     4381 catcgaggcc cacgagctcg acacattcgt cgccgctttg cccggtctgc tcgacgtcgc
     4441 gctggccggt gacccgccgt gatccccgca acgccgaccg cccgtcgggc caagatcgcg
     4501 gggctgctcg ccgaccgctc ggtgcgctcg cagggcgagt tgctcacgct gctcgccgac
     4561 gcgggcatcg aggtcaccca ggcgacgctc tcgcgcgatc tggaagcgct cggcgcggtc
     4621 aaggtgcgcg ctggccgcgg ccttgtgtat gccgtccccg gcgcctcccc gtcgcccgtc
     4681 gagcaggtgc cgtcccggct gcgccggttg ttggaggagt tgctcgtcac cgctgacgcg
     4741 tccggcaacc tcgtggtgtt gcgcaccccg ccgggagccg ccaaccatct cgcgtcgact
     4801 atcgatgcgt cgcacctcac cgacgtcctg ggcaccatcg ccggggatga cacgattctg
     4861 ttggtcacgt cgtcggccaa gggcggccgg gcggtcgcga cgcggctgct cgccctggcc
     4921 ggggcgtcgg cctgacgtgg cggcggacct catgcacgac cagaatctcc gtgcacccgt
     4981 gagcgatccg gtgcacccat gaacacagaa aggcccggta tgccgcacga ggactccgct
     5041 ggaacgagcg cccgagtgag cctgtggggt ggtcggttcg ccggcggtcc ggccgatgcc
     5101 ctcgccgaac tctcccgcag cacgcacttc gactggaggc tcgcggcata cgacattgcc
     5161 gggtctcagg cacacgcgcg ggtgctgcat cgcgctggtc tgctgtccga cggtgacctc
     5221 gcgacgatgc ttggggcgct ggacgagttg ctcgtcgacg tccacacggg ggcgttcgtg
     5281 ccttcgccgg aggatgaaga cgtgcacacc gcgctggagc gcggcctgat cgagcgggct
     5341 ggcgcggacg tcggcgggcg gttgcgtgcg gggcgctcgc gcaacgacca gatcgccact
     5401 ttgctgcgga tgtgtctgcg ggacgcggct cgtgacctcg ctgcgcaggt gctcgatctg
     5461 gtcgatgcgt tggtcggtca ggcgcagtct catctcgggg tagcgatgcc ggggcggacg
     5521 cacatgcagc acgcgcaacc ggtgctgttg tcgcatcatc tgctggccca cgcgtgggcg
     5581 ctgttgcgtg atgtcgatcg gctgcgcgac tgggaccgac gcgccgactc ctcgccgtat
     5641 ggctctgggg cgttggcggg gagttcgctc gggctggacc cggaggcggt ggcccgtgac
     5701 ttggggttct cgcggtcggt cgagaactcg atcgacggga cggcctcgcg cgacgtggcg
     5761 gccgaattcg cgttcgtgat ggcgatgacg gcggtcgacg tgtcccggat cgccgaggag
     5821 atcgtcgtgt gggcgaccaa ggagttcgga tacgtcacgc tcgacgacgc ctattcgacg
     5881 gggtcgagca tcatgccgca gaagaagaac cccgacatcg ccgagttggc tcgagggaag
     5941 gccggtcggg tcgtcgggga cctgactggg ctgctgacga cgctcaaggg cttgccgctg
     6001 gcgtacaacc gggacctgca ggaggacaag gagccggtgt tcgacgccat cgacaccttg
     6061 gcggtgttgt tgccggcctt caccgggatg gtccagaccc tggtcttcaa ccgtgagcgt
     6121 ttggaaagcc ttgcgcccca agggtttgcc ctggccacgg atatcgccga gtggttggtc
     6181 cgtcaggggg tgcccttccg ggtcgcccat gaggtggccg gtgcgtgcgt gcgtgagtgc
     6241 gagggccggg ggatcgagtt gtgggagctg acggatgccg agcttgcggc ggtgaatccg
     6301 gctctcacgc cggccgtgcg tgaggtgctc agtgtggccg ggtcgctggc ctctcgggac
     6361 gctcgtgggg gcaccgcccc ggtccgcgtg ggcgagcaac tcgccgaagc ccaggctgcc
     6421 gtcgcctccg cccgagcctt cgccgctccc gcctgaccca ttgccgtcgc ccgcccccct
     6481 ttgccgggtc gaggtattcc gccatcgtca ggtgatagcg aaatacctcg acccgctgtt
     6541 ggagctcggc gggccggaaa gatgaccgtg cgagcgctat ctccgggtcg aaacgaggct
     6601 gggggccggg aagatgaccg tgcgagcgct atctccgggg tgggtcaggg ctagttgcga
     6661 ggggaactgt ggaaggcggg gtcggcccgc agcgccatga actccagggc tcgctcctga
     6721 gcggacaggt cgactgccgc cgtcggcgtc cacgtgggca cgggacacgg ccgcccatcg
     6781 gcatccagcg cgacatagac gctcgtgcac tgggttgcca gagccaactc gccggtgcgg
     6841 gggctgccgg ccaacacccg cagcgcgaca tgcatcgagg tccgccccgt gtgcaccagc
     6901 cgtgactgga tctcgacgac gtcgccgatc cggatgggtc gcaggaacga caccccaccg
     6961 gagaaccgcg acaccacgtg ggtctggctc catcccgagg cgcacacgta gccggtctca
     7021 tccagccagc gcatgaggat cccgccatgc accttgccgc cccagttaac gtcggtcgga
     7081 gcggcgagga agcgcagcgt cgccccgtga gccagcgtcg ctccctccca gtcctgcgcg
     7141 gccaccagtg acttgacccg ctcgcgcagg tcgatccgcc gccgcgcctc ggcctgagca
     7201 gcacgcagca ccgcgccggg cgggtcccat tgggccacgt caaccgtccg cccctcgtcg
     7261 tcgacggcca cgaacaccat ccgacactcg aacgcgtgct ccacctgccc gccgcgcggg
     7321 ttccccgacg acacgtccac gacgatcgtc atgctcgaac gcccagtgag gaccagttgc
     7381 gccgacacct ccaccaggtc tcccacctgc accggccggt ggaagtcaat gttccccacg
     7441 tatgccgtca cgcaatagcg ccccgaccag cctgcggcac aagcgaaccc ggccttgtcg
     7501 atccattcga ggatccgacc tccgctcacc gacccgcctt ggcccgcgtc agtcggtgcg
     7561 gcgaggaagc gcagggtgac ctggcccgtg gtggcggtgg acggcatacc ggagagtatg
     7621 ccgcgcggct gcgcgagact gtcggcgtgt cccgcctgcc cagtggcttc tacgaccggc
     7681 ccgtgctcga cgtggccgtc gacctgctcg gcgcgcgaat ctcgcacggc ggcgtgacga
     7741 ttcggctcac tgaggtcgag gcgtatgccg gggtcggaga cccgggatcg catgccttcc
     7801 gaggtgagac acctcgcacg acggtgatgt tcggagcccc gggaggcttg tacgtctatt
     7861 tcacctacgg catgcactgg tgcgccaacc tggtgtgtgg accggacggc caggcttcgg
     7921 cggtcctcat gcgcgccggc gaggttgttg acggagagga gatcgcccga acccggcgcg
     7981 gggaggcttg tgcgacaagg gatctcgccc gtggcccggc tcggctcgcg tcagcgctcg
     8041 ggctgaccgg ggaagtcaac ggcacgctga ccttcgagcc cgacagcccg gtgagggtgc
     8101 aggcagcggc tgaggcgcca gacccggccc gcatccgcac gggaccccgc gtcggggtca
     8161 gtggcactgg gggtgacggg acggcatacc cctggcggtt ctggatcgac ggcgaaccca
     8221 cggtgtcggc ctatcgcccc ggcaagccga gacggcgccc ctgacgcggg ggccgatcga
     8281 catcagcgca ggatcgcgtg cagcgtgcgg cgcatgacat cggccagcgg atgccgccgc
     8341 ggcgtgtaat agatgacgcc ggccaccgcc tgttgcaggg cgtagccgcg tgcccgcagc
     8401 cacgagtcat cgtccgctgc agcgccgtcc cggaagcgct cgcgcccctg cggaccgaga
     8461 acagcccagg cagggacgag atcgagcgcg gggtcgcccg cccccgcacc gccccagtcg
     8521 atcaccgcgg tgagccgatt gccttgcacg agaaggttgc ccgggatcag atcgccgtgg
     8581 cagaggacgg cgtcaccgtc ccactcccgc gcggacaacg actcctgcca cgacgcgcgg
     8641 acgccgcgct cgtcgacgag gccgtgcgcc tcacccaacc actcgtcgat gtcctcagtc
     8701 agcggggaga gcccgccccc acgtcgccca ggggcgcgga cctcgacgat gtccccgtat
     8761 gccgcccgcg gcaccttccc gagggcgtcc accaccgccg cgaggtcctc cgcgagccgg
     8821 aagccgtgtg gatcgtcaag cgttgcccga tcggcccagg cgtcccgtcc gggcaaccat
     8881 cgcagcaccg cccaggggtg ggggtagcgc gcgctcgcgg aaccgaggtg gaccagttcg
     8941 ggaagggcga ctgggagccg cccagcaagg tgggggagca gccgcgtttc ggtgcgcagg
     9001 ctcgtggcgg ccgacggagt gcgaggaagc cgcacgacca actccgttcc gagccgatac
     9061 agcacgttgt ccgatccggt gttgccgtcg gtgccgctga ttcgcgcgag ggagagcgcc
     9121 gccaggtggg gcgcctggct tcggagcagg tcgagcacga cctccaagcc cgtgtcggct
     9181 tcgtcgtcgt gcagcggttc catcggctca tcccaccagt cggtggttcc cgacgccagc
     9241 gcgtttcctt tggtctgcag gtctgcaggt ctgcaggtct gcgggtctgc agccggcgcg
     9301 cggcgcgcct tagtctcgag tggtgaaccc gatgcgcgcc ctggcgatcc ggctcggcgc
     9361 ccagccctgg ttgccgcgcc tcgccccggt ggtgctgggg ctcgacaagg cgttgttcgc
     9421 cgccacccat gggcgagccg gcgtgctgtc gatgtctggg ctgccgtgga tcacgctcac
     9481 cgtcgtcggc cggcgcaccg ggctcgtcag gagggtgccg ttgctcgccg tcccgcacga
     9541 cgacggatgg ctcgtcgccg gctcgaactg gggtggtccc gcgacgcccg cttgggtcgc
     9601 caacctgact gcggcaggcc gcgccgtcgt cacccgaagc ggccgggaca cgcccatgag
     9661 cgcgcggcgg gtcacgggtt ccgagcgtgc ccaactctgg gccgagatgc tcgctacttg
     9721 gcccaacttc gagcgttacg cccaacggac cacccgggag atcccggtct ttgtgctcac
     9781 gcaggtcggt ccgacggtcg cgcacgtgcc ggcgcgggcg cggcataccg gcgggcgata
     9841 cctgttcgcg aggggcggac ggcatacggc aggcttgacc cagccagact caacccggac
     9901 cgaagggaac ccacccccgt gagcgacatc ctcgacgagc tgcagtggcg cggctcggtg
     9961 gcgcagacca ccgacgaaac cgcgctgcgc gaagccctcg cgagcggacc gatcacggcc
    10021 tattgcgggt tcgacccgac agcgccgtcg ctgcacttcg gcaacctcgt ccagctcgtc
    10081 ctcctgcgtc ggctacagcg ggccggccac cgcgtcatct gcctggtggg aggctcgacg
    10141 ggtctcgtcg gcgacccgcg ccccaccgcc gagcgggtgc tcaagaccaa ggaagaggcc
    10201 gccgccaacg tcgcccggat caaggcgctc gttcggccgt tcctcgactt cgacggcact
    10261 gacccggcag ccgcgcatcc cgcgatcctc gtcgacaacc tcgactggac cgctccgatg
    10321 tcggcgctgg acttcttgcg cgacatcggc aagcacttcc gggtcaacca gatgatccgc
    10381 aaggagtcgg tggcgcgccg gctggagagc caggagggca tctcctacac ggagttcagt
    10441 taccagctct tgcaggcgct ggactacctc gagctctacc gggtccacgg ctgcacgctg
    10501 cagaccggcg gctccgacca gtggggcaac ctcaccgccg gcgtcgacct catccaccgg
    10561 gcggaggggg tctcggtgca cgcgttggcg acgccgctcc tcaccgactc ctccggctcg
    10621 aagttcggca agtccgaggg caacgcagtc tggctgtcgg cggacatgac cagcccctac
    10681 gccttctacc agtacttcct caacgtcgag gacgcctcgg tgatcagcct gctcaaggtg
    10741 ttcagtgacc tgggggccga cgcgatcgcc gagctcgaga cggcggtggc ggcggagccc
    10801 ttccgccgcg cggcccagcg cacgctggct gccgagatca cgaccctcgt gcatagcagc
    10861 gccgccaccg cggcggccca ggcggcctcg gaggcgatct tcggcaaggg tgatctcgcg
    10921 gctctcga
//
LOCUS       DAOMZY010000082        10812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5700_length_10812_cov_6.035140, whole genome shotgun sequence.
ACCESSION   DAOMZY010000082 DAOMZY010000000
VERSION     DAOMZY010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 10812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10812
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5700_length_10812_cov_6.035140"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            339..821
                     /gene="rpsP"
                     /locus_tag="PLK64_06320"
     CDS             339..821
                     /gene="rpsP"
                     /locus_tag="PLK64_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPV79392.1"
                     /translation="MAVKIRLKRMGKIRTPFYRVVVMDSRTKRDGRAIEEIGKYHPTE
                     EPSVIDIDSARAAYWLAQGAQPTEAVEALLKVTGDWQKFKGIDGTEGTLRVAAPKTSK
                     KDLYEAAIAAAGKSLELKGSATTVRRKPAKAEAAVAEITEALAEADKPAEAAAEGEEA
                     "
     gene            824..1063
                     /locus_tag="PLK64_06325"
     CDS             824..1063
                     /locus_tag="PLK64_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPV79393.1"
                     /translation="MLEEALEHLVKGIVDHADEVVVRRKELRRGELLEVRVHPEDLGR
                     VIGRSGRTASALRTVMSALSGGSNIRVDIVDTDRR"
     gene            1078..1608
                     /gene="rimM"
                     /locus_tag="PLK64_06330"
     CDS             1078..1608
                     /gene="rimM"
                     /locus_tag="PLK64_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142763.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPV79394.1"
                     /translation="MAHVVARIGKPHGIRGEVTVQLHTDDPAGRFAVGTVLPTKAPKG
                     SGVPLELTIASSRDHNGVWLLGFEEIPDRTGAEGLRGTRLLTPEGTEELLEPDQGGDA
                     WYEEQLVGLAVVLKDGSAVGTVAGLDIGPGQDRLRVARDGGRTAYIPFVSALVPVVDV
                     VGGRVVIDPPPGLLEL"
     gene            1616..2308
                     /gene="trmD"
                     /locus_tag="PLK64_06335"
                     /pseudo
     CDS             1616..2308
                     /gene="trmD"
                     /locus_tag="PLK64_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493114.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
     gene            complement(2336..2722)
                     /locus_tag="PLK64_06340"
     CDS             complement(2336..2722)
                     /locus_tag="PLK64_06340"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPV79395.1"
                     /translation="MSVTPPTRSTPIRFGRTAPGIPVRDIEQALAFYRDALGMTVTFT
                     NGDPVGFVILERDAAELHLTLVPTHRAGTHNVAHLMVSDAAAVHDRLVKHGARIVKGL
                     RDHDFGLRAFVVADPDGNRLDIAEEL"
     gene            3039..3416
                     /gene="rplS"
                     /locus_tag="PLK64_06345"
     CDS             3039..3416
                     /gene="rplS"
                     /locus_tag="PLK64_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPV79396.1"
                     /translation="MHQLDDVNAATLRDDVPAFRAGDTLKVHVKVIEGTRSRIQVFQG
                     VVIRRHGAGVGETFTVRKVSFGVGVERTFPVHTPIIEKIEVVTRGDVRRAKLYYLRDL
                     RGKAAKIKEKRDTHGKFEAKNGA"
     gene            3479..4192
                     /gene="lepB"
                     /locus_tag="PLK64_06350"
     CDS             3479..4192
                     /gene="lepB"
                     /locus_tag="PLK64_06350"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015937400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPV79397.1"
                     /translation="MTRREVREGRARSKRAAGDRIPESVKIGVIAIAVMLLVRALVVQ
                     TFSVPSGSMQETLEPGDRILVSRLQRGPSIERGDVVVFDGTDTWGKPAGSDTASGPRQ
                     LLTSILAIVSLSSGADYVKRVVGVPGDHVVCCDVDGRLTINGVAVNEPYLYPGNPPST
                     TRFDVTVPAGKIWVMGDHRSASADSRSQMGKPGGGMVPLDDVVGRAWVRYWPLDRLGS
                     LTPAPTLHDLPLPTGAAPS"
     gene            4189..5022
                     /gene="lepB"
                     /locus_tag="PLK64_06355"
     CDS             4189..5022
                     /gene="lepB"
                     /locus_tag="PLK64_06355"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPV79398.1"
                     /translation="MTDESPPAAPGSDLVQRGFWRSVWVGVREVVIVVSLAMALSLVV
                     KTFLVQPFHIPSGSMEDTLIKDDRVLVSKLTPGPFELSRGDIVVFTDPGHWLGEIPEV
                     HRTPVQSALIFLGLAPDDSHEHLIKRVIGLPGDHVVCCTVGGQLTVNGVAITEPYLKP
                     GDNAAGGRGSFDIIVPAGKVWVMGDHRSDSADSRFHDDGTGSTGSVPIADIRGKAVLV
                     VWPIDRWAGLSEFGDVFATVPDPGKAAPGDSVVTPSSSPDSSSSRDSSSSRDSAGPAA
                     S"
     gene            5031..5813
                     /locus_tag="PLK64_06360"
     CDS             5031..5813
                     /locus_tag="PLK64_06360"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPV79399.1"
                     /translation="MSAARTSSPRTTSPRPTSRSRTPSLRVERGLQRQGYRLLAGMDE
                     VGRGALAGPVSVGVVVIDEVCRSAPAGVRDSKLLSAPARERMVAPIRRWAHAHAVGHA
                     SPVEIDQIGIIAALRLAGVRALAALPVTPDLVILDGNHDWLTAPEQVGLLGFVQDDES
                     EATRTPPVTTMIKADLRCSSVAAASVLAKVERDGLMVALGGEHPAYRWEINKGYAAPE
                     HLAALDLLGPCDLHRRSWRLRGPGSEVVERTDPTDSAPMRTR"
     gene            5810..6115
                     /locus_tag="PLK64_06365"
     CDS             5810..6115
                     /locus_tag="PLK64_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 domain-containing protein"
                     /protein_id="HPV79400.1"
                     /translation="MSSEDLEKYETEQELSLYREYRDVVSLFSHVVETERRFYLCNSV
                     DMKVRSDGGEVFFDITMNDAWVWDIYRPARFVKSVRVVTFKDVNIEELPKSELEMPS"
     gene            6219..6614
                     /locus_tag="PLK64_06370"
     CDS             6219..6614
                     /locus_tag="PLK64_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPV79401.1"
                     /translation="MQTSEGMPHRVAAAVGDYGERMAERYLRDQGLEILARNWRCALG
                     EIDIVARDGGCLVVCEVKTRRGVAFGGPIQAVTLRKLARLRRLAAAWLHEREGDAAGS
                     HFPDVRVDVVGVARPRSGPCVIEHLVGVA"
     gene            6616..8154
                     /locus_tag="PLK64_06375"
     CDS             6616..8154
                     /locus_tag="PLK64_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPV79402.1"
                     /translation="MGLGRTRSVGLRGVEGFLVEVESDVAPGLPAFTVSGRADAACAQ
                     APDRVRAAASNSRHPVPQRRITVNLSPASVLKVGSGFDLAIVVATLAAAGEIPAGVVE
                     EVVHIGELGLDGSVRPVRGLVPLVVAAAKAGARHVVVPVGNAKEARLVAGVQVHPVAW
                     LEELVARYRTLAHGGPVSDVPEPDALSEPERPAPDLADVVGQAEGRLAVEIAAAGAHH
                     LYLVGPPGAGKSMLAERLPGLLPELDDHQAMEVTAIASILGTLASFDGLCRRPPFVAP
                     HHGASMPAIIGGGSGHVLPGAVTAAHHGVLFLDEAPEFRPGVMQALRQPIESGHVVVA
                     RAIGNIRFPCEFQLVLAANPCPCGMGSGKASSCSCTPIQQRRYSGRLSGPILDRIDIQ
                     VHVPAPSRAALLGEPGESSEVVRARVFQARATASLRWDARPYAVNGRVPGPVLRSGPF
                     RLGPTRTVDLDRAIDRGLLSLRGYDRCLRLAWTIADLAGRTSPTRDDVGLALSLRHQP
                     ALAA"
     gene            8180..9367
                     /gene="dprA"
                     /locus_tag="PLK64_06380"
     CDS             8180..9367
                     /gene="dprA"
                     /locus_tag="PLK64_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPV79403.1"
                     /translation="MSVLPTSLTQDRWARAAWSRVAEPGDRRAVGLVETLGAVDALAA
                     VVENRPWAPEALRTRVAGLDLDEVVQFARRQGWRMVVPGDAEWPSGLDALGAPPIGLW
                     VDGPADLGGVCIGSVAIVGARACTAYGMGVASELGAGLPDAGYAVVSGAAFGIDRAAH
                     AGTLSVRGTTVAVLAGGLDRPYPVSNSELIAKIRATGALVSEVAPGGAPTRSRFLQRN
                     RLIATMTVGTIVVEAGLRSGSLHTAGQAADHGRPVGVVPGPVTSRVSAGCHQWIRDGK
                     GVLVTDVAEVLDLVGRLGVDAAPHRSGPVRPEDELPPQDRLVLAALPVRAPAAIPTVA
                     AHAGLDVGQTLASLGRLELAGVAVRVGDRWKQTPGRLTQSPDAEAVADVGGREVDAPA
                     TPG"
     gene            9371..10375
                     /locus_tag="PLK64_06385"
     CDS             9371..10375
                     /locus_tag="PLK64_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HPV79404.1"
                     /translation="MPWPEMCDGWDVHEDEVGPQSDAPAQAGLEGAAWAGTVLVDYAR
                     YLDAERGLSVHTCRAYLGDIRALLGYLGRSRGVTALSEIELGDLRGWLAAMSRESSAA
                     RTSVARRAASARTFFRWATRTGRISVDPSLRLVAPRRTGHLPGVLRQSEATELMAVAE
                     LAADDGDPISARDRAMVELLYASGLRVSELAGVDVADVDFTVGVVRVLGKGGKERVVP
                     FGLPAATALREWAAYRPLLATERSGAALFLGRRGRRVDPRQVREAVHRLVAHVPGAPD
                     LAPHGLRHSAATHLLEGGADLRTVQELLGHASLATTQIYTHVSAERLRKSYEQAHPRA
                     "
     gene            10497..>10812
                     /locus_tag="PLK64_06390"
     CDS             10497..>10812
                     /locus_tag="PLK64_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79405.1"
                     /translation="MAPTETALRRADLRAAGSTTPASDVTTTRRAANRRRSARRRRMH
                     RLRRTATLALGLLFVGTVVARGAQDPAVALPRGVQSASSSTALPGTLGSAADVARAGT
                     APA"
BASE COUNT         1514 a         3721 c         3882 g         1695 t
ORIGIN      
        1 cccgcggcat acccggtggg agttgggccg gtgttgcggg tcgacacctc ccagtcggtg
       61 accgacgcgc aggtatgccg cctcgccctc gccatccgag ccgccggcac cccaactggg
      121 tcgaggtatt ccgccgttat ctgacgatgg cggaatacct cgacccggca gggggtccgg
      181 cagggggtgg cgcggcatac ctgccaggtc tggcagaatg ggcggctgta ctcgccgcac
      241 ccaccaggga gcgtgcatct acacgccgag tgacggcacc ccggagcgac ttgccccggt
      301 ctcgccgtgc tcacgatacg aagcaggaga ccactaccgt ggccgtcaag attcgcctca
      361 agcggatggg caagatccgc accccgttct accgtgtcgt cgtcatggac agccgcacca
      421 agcgtgacgg ccgggcgatc gaggagatcg gcaagtacca ccccaccgag gagccctcgg
      481 tcatcgacat cgactccgct cgcgccgcct actggctggc ccagggcgct cagcccaccg
      541 aggccgtcga ggcgctgctc aaggtgaccg gtgactggca gaagttcaag ggcatcgacg
      601 gcaccgaagg cacccttcgc gtcgccgccc cgaagacgag caagaaggac ctctacgagg
      661 ccgccatcgc tgccgcgggc aagtcgctcg agctcaaggg ctcggccacc acggttcgcc
      721 gcaagccggc caaggccgag gctgccgtcg ccgagatcac cgaggcgctc gccgaggccg
      781 acaagccggc cgaggccgct gccgagggcg aagaggcctg accgtgctcg aagaggccct
      841 ggaacacctg gtcaagggca tcgtcgacca cgccgacgag gtcgtcgtgc gccgcaagga
      901 gctccgtcga ggcgagttgc ttgaggtgcg cgtgcacccc gaggacctcg gccgagtcat
      961 cggccgctcg ggtcgcacgg ccagcgcgct gcgcacggtc atgtcggccc tgtccggcgg
     1021 ctcgaacatt cgcgtcgaca tcgtcgacac cgaccggcgc tgaggctcag ccctctcgtg
     1081 gcgcacgtcg tcgcccggat cggcaagccg cacggcatcc gcggggaagt cacggtgcaa
     1141 ctgcacacgg acgaccccgc gggccggttc gccgtcggca ccgtcctgcc caccaaagca
     1201 cccaagggca gcggtgtccc gcttgaactc accatcgcgt cctcgcggga ccacaacggc
     1261 gtctggctgc tggggttcga ggagatcccc gaccgcaccg gcgccgaagg cctgcgcggc
     1321 acccgcctgc tgacccccga gggcaccgag gaattgctcg agcccgacca gggcggagac
     1381 gcctggtacg aagagcaact cgtcggcctc gccgtcgtgc tcaaggacgg gtcggcggtc
     1441 ggcacggtgg ccggcctgga catcggccca ggtcaggacc ggttgcgcgt ggcccgcgat
     1501 ggtgggcgga cggcatacat cccgtttgtg tccgcgctgg tcccggtcgt cgacgtggtc
     1561 ggtggacggg tggtcatcga cccgcccccg gggctgctgg agctctgagc ccgtcatgcg
     1621 gatcgacgtc gtgacgatct tcccggacta cctcgcgccg ctggagctct cgctcatcgg
     1681 caaggcgcgc gagcaggggt tgctcgacgt gcgcgtccac gacctgcgcg agcacgccca
     1741 cgaccgccac cggaccgtgg acgactcgcc ctatggcggg ggagccggca tggtcatgcg
     1801 ccccgagccg tggggcgagg ccctcgacgc gctggtcgct tccgcgcccg acgccgcccc
     1861 gccgcgcctg ctcgtcccca ccccatccgg tatgccgttc acccaggcca tggcgcgcga
     1921 cctcgccgcc gagccgtggc tggcgttcgc gtgcggccgt tacgaaggca tcgacgagcg
     1981 ggtcatggag tatgccgcgc agcgcatgcc cgtgacgctc gtgtcgctcg gggactacgt
     2041 cctcaacggg ggagaggtgg ccgtgctggc catcgtcgag gccgtcgcgc gcctgctgcc
     2101 cggcgtcatc ggcaacgccg agtcgctcgt cgaggagtcg cacgaggacg ggctgctgga
     2161 ataccccgtc tataccaagc cggcctcttg gcgcggtctg gacgtgcccg acgtgctgct
     2221 ctcgggacac cacggcgcga tcgcggcctg gcggcaggcg cagcgcgtcg agcgcaccgc
     2281 cacccgccgc cccgacctgc tgcctcccga cctgcttccc cctgactagg ggccctcaga
     2341 gttcttcggc gatgtccaag cggttgccgt cggggtcggc gacgacgaag gctcgcagcc
     2401 cgaagtcgtg atcgcgcagc cccttgacga tccgcgcccc atgcttgacc aggcggtcat
     2461 ggaccgccgc cgcgtcgctc accatgaggt gggcgacgtt gtgcgtcccg gctcggtgcg
     2521 tcggcaccag cgtgaggtgc aactcggcgg cgtcgcgctc aaggatgacg aaaccgacgg
     2581 ggtcaccatt ggtgaaggtc accgtcatcc cgagggcgtc ccgatagaac gcgagggcct
     2641 gctcgatgtc gcggacaggg atgccgggag cggtgcgccc gaagcggatc ggcgtgcttc
     2701 tggtcggggg agtaacggac atggcggtct ccatactttc gagtcgccga ctcgggagcc
     2761 ctggctgagg cggtgcccaa ccaggcgagc ggcgggagac ggggcggtgc ccgcgatgac
     2821 ggggtgatcc tagccatgat ttcccggtat gccgcgtggt ctggcacact tgggcagttg
     2881 ccttccagca tctgacgagc ccctgccaca gggggagcga tccggaccac ccgatcttcg
     2941 ggagggccgt cgcgcggcca gccggacggc ataccgaaag ctctgaacca gccacccccc
     3001 gaggcgacct gtggcgccgc gagaaagcag atgatcccat gcaccagctt gatgacgtca
     3061 acgccgcaac tctgcgcgac gacgttcccg ccttccgcgc cggtgacacc ctcaaggtgc
     3121 acgtcaaggt catcgaaggc acccgttccc gcatccaggt cttccagggc gttgtcatcc
     3181 gtcgccatgg cgcgggcgta ggcgagacgt tcaccgtgcg caaggtgtcc ttcggcgtcg
     3241 gggtcgagcg aaccttcccg gtgcacaccc cgatcatcga gaagatcgag gtcgtcactc
     3301 gtggtgacgt gcgccgcgcg aagctctact acctgcgcga tctgcgcggc aaggccgcca
     3361 agatcaagga gaagcgcgac acccacggca agttcgaggc caagaacggc gcctgaggcg
     3421 agtggacgag gcctcggggg gcctgccgcg tgagcctgat ggtggcaacg cgcggccgtt
     3481 gacacgccgc gaggtgcgtg aagggcgggc acgcagcaag cgtgctgctg gcgaccgcat
     3541 ccccgagtcg gtcaagatcg gcgtgatcgc gatcgccgtc atgctcctcg tccgcgcgct
     3601 ggtcgtccag accttctccg tcccctcagg ctcgatgcaa gagactctcg agccgggcga
     3661 ccggatcctc gtcagtcggc tgcagcgagg gccgtcgatc gagcgcggcg acgtcgtcgt
     3721 cttcgacggg accgatacgt ggggcaagcc tgccgggtcc gacactgcct cggggccccg
     3781 tcaactgctg acctcgatct tggccatcgt gtcgctgagt tcgggcgctg actacgtcaa
     3841 gcgggtcgtc ggcgtgccgg gtgaccacgt tgtgtgctgc gacgttgacg gtcggctgac
     3901 catcaacggg gtggccgtca atgagcccta cctctacccc ggaaacccgc cgagcacgac
     3961 gaggttcgac gtcacggtgc cggccgggaa gatctgggtg atgggcgacc accgcagtgc
     4021 gtcggccgat tcgcgatcgc agatgggcaa accgggcggc gggatggttc ccctcgacga
     4081 tgttgtcgga cgtgcgtggg ttcggtactg gccgctcgat cgactgggta gcctgactcc
     4141 tgcccccact ctgcacgatc tcccactgcc caccggagcg gcgccttcat gaccgacgaa
     4201 tctccgcccg ccgcaccagg ctccgacctg gtgcagcgcg gcttctggcg cagtgtctgg
     4261 gtgggtgttc gcgaggtcgt catcgtggtg tccctcgcga tggccctctc gcttgtcgtc
     4321 aagaccttcc tggtgcagcc cttccacatc ccctccgggt cgatggagga caccctcatc
     4381 aaggacgatc gggtcctggt cagcaagctg acgcccgggc cgttcgaact gagccgaggg
     4441 gacatcgtcg tcttcactga ccccggccat tggctcggcg agatccccga ggttcaccgc
     4501 accccggtgc aatcggcgct gatcttcctc ggattggccc ccgacgactc ccacgaacac
     4561 ctcatcaagc gcgtcatcgg gctgccgggt gaccatgtcg tctgctgcac cgtgggtggg
     4621 caactcaccg tgaacggcgt cgccatcacc gagccctacc tcaagcccgg cgacaacgcg
     4681 gcaggcggcc ggggttcctt cgacatcatc gtccctgccg gcaaggtctg ggtcatgggg
     4741 gatcaccggt ccgactcggc cgactcccga ttccacgatg acggcaccgg ctccacgggg
     4801 tcggtcccga tcgccgacat ccgcggcaag gcggtgctcg tcgtctggcc gatcgatcgg
     4861 tgggccgggc tgtcagagtt cggcgacgtg ttcgcgaccg tgcccgatcc gggcaaggcg
     4921 gcgccgggag acagcgtcgt cactcccagc agcagccccg actcaagcag ttccagggac
     4981 tcaagcagtt ccagggactc cgccggtccc gccgcgtcct gagcccccac atgtcggccg
     5041 cacgcacgtc ctctccacgc acgacctccc cacgcccgac gtcacggtcg cgcacgccct
     5101 cactgcgcgt cgagcgaggc ctgcagcgtc agggctaccg gttgttggcc gggatggacg
     5161 aggtgggtcg aggcgccctg gccggcccgg tgagtgtggg cgtggtcgtc attgacgagg
     5221 tatgccgctc cgcccccgcc ggggttcgcg actccaagct cctctccgcc ccggcccgcg
     5281 aacgcatggt cgccccgatc cgtcggtggg cccacgcgca cgcggtgggg catgcctcgc
     5341 ccgtagagat cgaccagatc gggatcatcg cggcgttgcg gctggccggc gtccgcgcgc
     5401 tcgcggcgct gccagtgacc cccgacctgg tcatcctcga cggcaaccat gactggctga
     5461 ctgcacccga gcaggtcggg ttgctgggct tcgtgcagga cgacgagtcc gaagccaccc
     5521 ggacgccgcc ggtgacgacg atgatcaagg ccgacctgcg ctgctcctca gtggccgccg
     5581 cgtcggtgct ggcgaaggtg gaacgcgacg gtctgatggt ggcgctggga ggcgagcacc
     5641 cggcataccg gtgggagatc aacaaggggt atgccgcgcc cgaacacctc gccgcgctcg
     5701 acctgctcgg gccctgcgac ctgcaccggc gcagttggcg gctgcgtgga cctggctccg
     5761 aggtggtaga acggaccgac ccgaccgata gcgcgccgat gaggacccga tgagctccga
     5821 ggacctggag aagtacgaga ccgagcagga actctcgctc taccgcgagt accgcgacgt
     5881 cgtgtcgctg ttctcccacg tggtcgagac cgagcggcgc ttctacctgt gcaactcggt
     5941 cgacatgaag gtgcgctccg atgggggcga ggtgttcttc gacatcacga tgaacgatgc
     6001 gtgggtgtgg gacatctatc ggccagcgcg gttcgtcaag tcggtgcggg tggtgacgtt
     6061 caaggacgtc aacatcgagg aactgcccaa gtctgagttg gagatgccct cctagcgatc
     6121 ggcccggctg tccacatggg gtcgtacgtt cgtcccactg tccacagggc aggaggtggg
     6181 ggtgggcggc atacccgcgg ggctgtttcg cttcgggcat gcagacatcg gagggtatgc
     6241 cgcaccgggt cgccgccgcg gtcggtgact atggcgagcg gatggccgag cgctatttgc
     6301 gcgatcaggg tttggagatc ctggcccgca actggcgctg tgccttgggc gagatcgaca
     6361 tcgtggcgcg cgacgggggt tgcctggtcg tgtgcgaggt caagacccgc cgtggggtgg
     6421 cgttcggtgg cccgatccag gcggtgacat tgcgcaagct cgcccggctg cgccgattgg
     6481 ctgccgcgtg gctgcacgag cgcgagggcg atgcggccgg gtcacacttc cctgacgtgc
     6541 gggttgatgt cgtcggggtg gcccgaccgc gcagcggccc ttgcgtgatc gagcacctcg
     6601 tgggggtggc ctgagatggg gttggggcgg acgagatctg tggggctgcg aggcgtcgag
     6661 gggttcctcg tcgaggtcga gtccgacgtc gcgccggggc tgccggcgtt caccgtgagt
     6721 ggacgggctg acgcggcctg tgcccaggcg cccgatcggg tgcgggcggc ggcctccaac
     6781 agtcggcacc cggtcccgca acgacggatc accgtcaacc tgtcgccggc gtcggtgctc
     6841 aaggtgggca gtgggttcga tctggccatc gtcgtcgcga ccttggccgc cgccggagag
     6901 atccccgccg gcgtcgtcga ggaggtcgtg cacatcggtg agctgggtct ggacgggtcg
     6961 gtccgaccgg tccgagggtt ggtcccgttg gtcgtggctg ccgccaaggc cggtgcgcgg
     7021 cacgtggtcg tccccgtggg caacgccaag gaggcgcggc tcgtcgccgg ggtgcaggtc
     7081 cacccggtgg cctggctcga ggagctcgtc gcgagatatc ggacgctggc gcacggtggg
     7141 ccggtgtccg acgtgcccga gccggacgcc ctgtcggagc ccgagcgtcc agcacctgac
     7201 cttgccgacg tcgtcggcca ggccgaaggg cgcctcgccg tcgagatcgc cgccgcgggt
     7261 gcgcaccacc tttacctcgt gggtccgccg ggcgcgggca agtcgatgct cgccgagcga
     7321 ctgccgggcc tgcttccgga gttggacgac caccaggcga tggaagtcac cgccatcgcc
     7381 tccatcctcg gcaccctcgc aagcttcgac ggcctgtgcc gacgcccgcc gttcgtcgcg
     7441 ccgcaccacg gcgcgtcgat gccggcgatt atcggtggag ggtccgggca tgtgttgccc
     7501 ggagcggtga ccgcggctca tcacggcgtg ctcttcctcg atgaggcacc cgagttccgt
     7561 cccggggtga tgcaggccct gcgccaaccc atcgagtccg gccacgtggt ggtggcccgg
     7621 gcgatcggca acatccggtt cccctgcgag ttccaactcg tcctcgcggc caacccctgc
     7681 ccgtgcggaa tggggtcggg aaaggcctcc tcgtgttcgt gcacgccgat ccaacagcgt
     7741 cgctattcgg gtcggttgtc cggccccatc ctcgaccgga tcgacatcca ggtccacgtc
     7801 ccggcgccct cgcgggcggc cttgttgggt gagccggggg agtccagcga ggtggtgcga
     7861 gcccgggtct tccaggcccg cgcgactgcg tcgctccgct gggatgcccg cccgtatgcc
     7921 gtcaacggtc gggtccccgg tcccgtcctg cgatcgggcc ccttccggct cggcccgaca
     7981 cggaccgtcg atctcgaccg cgcgattgac cgtggcctgc tgtcgttgcg gggctatgac
     8041 cgttgccttc gactggcgtg gacgatcgcc gacctggccg ggcgcacctc gccgacccgc
     8101 gacgacgtgg gtctggccct gtcgctgcgc caccaacccg ccctcgccgc atgaccgccc
     8161 ggcactgtca gggggcctca tgagtgtctt gccgacctcc ttgacccaag accggtgggc
     8221 ccgggcagcc tggtcccggg tcgccgagcc gggagatcgt cgggccgttg gcctcgtcga
     8281 gacactcggc gccgtggacg cgttggccgc cgtcgtcgag aaccgtccgt gggctcccga
     8341 ggcgctgcgg accagggtgg ctgggctcga cctcgacgag gtggtgcagt tcgcccgccg
     8401 ccaggggtgg cggatggtcg tgcctggcga cgccgaatgg ccgagcggtc tcgatgctct
     8461 cggcgctcca ccgatcggcc tgtgggtcga cggcccggct gacctcgggg gcgtgtgcat
     8521 cgggtcggtg gcgatcgtcg gggcccgggc gtgcacggca tacgggatgg gggtcgcctc
     8581 cgagctgggg gccggccttc ccgacgcggg gtatgccgtg gtgtccggtg ccgcgttcgg
     8641 catcgaccgc gccgcccacg cgggcacgct gtcggtgcgc gggaccacgg ttgccgtgct
     8701 cgcagggggc ctggatcggc cctacccggt cagtaactcc gagttgatcg ccaagatccg
     8761 cgcgaccggg gctctggtgt ccgaggtggc tccgggcggg gcaccgaccc gatcgcgctt
     8821 cctgcagcgc aaccggctca tcgccacgat gaccgtcggg acgatcgtcg tcgaagccgg
     8881 attgcggtcg gggtcgctgc acaccgccgg gcaggcagcc gaccacggaa gaccggtcgg
     8941 cgtggtgccg gggccggtga cctcgcgggt gtctgcgggc tgccatcagt ggatccgcga
     9001 cgggaaggga gtgctcgtca ccgacgtagc ggaggtgctc gatctcgtgg ggcggctggg
     9061 cgtcgacgcc gcgccccacc gctccggccc cgttcgcccg gaggacgagt tgcctcccca
     9121 agaccggttg gtgttggctg cgcttccggt tcgagcaccg gcagcgatcc ccacggtcgc
     9181 tgcccacgcg ggcctcgacg tcggtcagac gctggcctcc ctggggcggc tggagttggc
     9241 tggggtcgcg gtgcgcgtcg gcgaccggtg gaagcagacc cccggacggc tgacgcagag
     9301 ccccgatgct gaggctgtgg cggacgtcgg cggccgggag gtggacgcgc cagcaacccc
     9361 cgggtgaggc atgccgtggc cagagatgtg cgacggttgg gacgtgcatg aggacgaggt
     9421 gggcccccag agcgacgcac cggctcaggc tgggctcgag ggtgccgcat gggcgggcac
     9481 cgtgctcgtc gactacgcgc gctacctcga cgccgagcgc gggctgtccg tgcacacttg
     9541 ccgcgcctat ctcggcgaca ttcgggcctt gctgggctac ctcggccggt cgcggggagt
     9601 gacggcgctg agcgagatcg agttggggga tctgcggggc tggctggctg cgatgtcccg
     9661 ggagtccagc gcggctcgca ccagcgtcgc gcgtcgggcc gccagcgcgc ggaccttctt
     9721 ccggtgggcg acccgcaccg gacggatctc cgtcgacccg tcgctgcggt tggtggcgcc
     9781 acgtcgcacg gggcacctgc ccggggtgtt gcgacagtcg gaggccaccg agctcatggc
     9841 ggtcgccgag ctcgcagctg acgacggcga tccgatctcg gcacgcgacc gggcgatggt
     9901 ggagttgctc tacgccagcg gcctgcgcgt gagcgagctg gcaggcgtag acgtggccga
     9961 tgtcgacttc acggtggggg tcgtccgcgt gctgggcaag ggtggcaagg agcgcgtcgt
    10021 gcccttcggt ttgcctgccg cgacagccct tcgggagtgg gcggcatacc ggccgctgct
    10081 ggcaaccgag cgcagtggtg ccgcactgtt cctcggccga cgtggccggc gcgtcgatcc
    10141 gcgccaggtg cgtgaggcgg tgcaccggct cgtcgctcat gtgccgggcg cccccgacct
    10201 ggcgccgcac gggctgcgac acagcgctgc gacccacctg ttggagggtg gggccgacct
    10261 gaggaccgtc caggagttgc tgggccacgc gagcctggcg acgacgcaga tctacacgca
    10321 tgtgtcggcc gaacggctac gcaagtccta cgagcaggct catccgaggg cctgagccgg
    10381 gaggcgtccc accggcgtgc ccgagccgct cgcgatccac cgatctggga cgatggtgcc
    10441 tcccgggtgc tgacaggcgg cccggtggtt ccctctctcg accttcagaa ggtggcgtgg
    10501 ccccgaccga gacggccctg cgacgtgccg atctgcgcgc cgccggttcg accacgcccg
    10561 cgtcggacgt gacgacaact cgacgggccg ccaatcggcg gcgctcggct cggcgccgtc
    10621 ggatgcaccg actgcggcgc acggcgacgt tggctttggg gttgctcttc gtcggcacgg
    10681 tcgtagcccg gggagcacag gacccggcgg tggcgctccc gcgcggcgtg cagtccgcga
    10741 gttcgtcgac ggcgctaccg ggaacgctcg gatcagcggc cgacgtcgcc cgagccggca
    10801 ccgcgcctgc gg
//
LOCUS       DAOMZY010000083        10792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5715_length_10792_cov_5.638353, whole genome shotgun sequence.
ACCESSION   DAOMZY010000083 DAOMZY010000000
VERSION     DAOMZY010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 10792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10792
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5715_length_10792_cov_5.638353"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="PLK64_06395"
     CDS             <1..557
                     /locus_tag="PLK64_06395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HPV79406.1"
                     /translation="GQALVPPTASDPAHAYAPAHPGVSVVKRINGDDAATAPGVSVSA
                     GSLMAITFEVTNTGDVRIEPVQVSDSTVTDIQCPGTALDPGEVMTCSATLAAPALGVE
                     HTNTVTVTGTPVLADGTPALGDDGQPASAPTATDTAYAVIVAPFLPNTGTDVARFLAI
                     ALGLMAAGGLLLAATRRRRRATEE"
     gene            700..798
                     /locus_tag="PLK64_06400"
     CDS             700..798
                     /locus_tag="PLK64_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HPV79407.1"
                     /translation="MGSVIKKRRKRMAKKKHRKLLRKTRHQRRNRK"
     gene            977..2029
                     /locus_tag="PLK64_06405"
     CDS             977..2029
                     /locus_tag="PLK64_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPV79408.1"
                     /translation="MANVVLITGVSRYLGGRFASALSKDSSIERIVGIDVVPPPHSIG
                     RAEFVRADIRNPMIGRIITQAKVDTVVHMNVLATPTAAGGRVSQKEINVIGTMQLLAA
                     CQKATSVKRLVVKSTAAIYGSSPRDPALFTEDTGPKTMPTSGFGKDSVEVEGYVRGFS
                     RRRPDAEITLLRFANVIGPGIKTAMTDYFSLPILPVPMGFDARLQFVHEDDATAAMLL
                     AVRGPAVQIVNIAGDGIITVTQAAAMVGRPAVPIPFGAAYLGSLVRRAGLADFSTDQL
                     SFLSYGRGLDTTRMREVMKFTPRHTTREAFLDFADHVPPVLPGADTAASLLGRAAGSA
                     LQSAGGLAGRIRQVAG"
     gene            2029..>2620
                     /locus_tag="PLK64_06410"
     CDS             2029..>2620
                     /locus_tag="PLK64_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79409.1"
                     /translation="MPGTSRDAFAAGAARAAGERRKRSSTPLVPADGMPPLPTDEARS
                     APHRPVPQVTPTASANATTVDAVARERRPRPVLAGLTNVGEATVTQLPTTRASDRAAG
                     STRLTPARRTGRRTHRESLPIAEGALTSAVSAPDTSITPELSSVAADQPAAATRVIEP
                     AVTAEAAGESAASTVQASTQASPRVRGDRTGTAYRSR"
     assembly_gap    2621..2720
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2721..3761
                     /locus_tag="PLK64_06415"
     CDS             <2721..3761
                     /locus_tag="PLK64_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPV79410.1"
                     /translation="QASPRVRGDRTGTAYRSRARTPVQAEISESLLASALTQQELTPD
                     IEDIVAAIIGVLRTVAAASGLGADEVEQAVARTLAFLRRRLTGDYSVDDFGLDSDYIE
                     NIFLPLMRVLYRQWFRVEVRGIENIPAEGSALIVANHSGTIALDSHMVQVAIHDEHPA
                     ARHLRLLGADLVFRTPIMGSLARKQGATLAANSDAERLLSAGEVVGVFPEGFKGVGKP
                     FSERYKLQRFGRGGFVSAAVRTGAPIIPCSIIGAEETYPLLGDVKVLARLFGAPYFPV
                     TPTWPWLGPLGLLPLPSKWIMEFGEPVETARYGAAGADDPMLVFDLTDQVRETIQQTL
                     YSLLMQRRSVFS"
     gene            3778..4053
                     /locus_tag="PLK64_06420"
     CDS             3778..4053
                     /locus_tag="PLK64_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HPV79411.1"
                     /translation="MFGRRKQSVRDAGTPRIVLVGRVGCHLCDEAEAIISRVAAETGA
                     TWVKVSVDDDPALLARWSDQVPVTLVDGRPHDFYRVSEARLRAALKS"
     gene            4214..4885
                     /locus_tag="PLK64_06425"
     CDS             4214..4885
                     /locus_tag="PLK64_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPV79412.1"
                     /translation="MVGTLAPRRDIPSATVSRLPVYLRALHEFATRDVTRVSSEELAA
                     QAGVRPTQLRKDLSHLGSHGVRGVGYDVGRLTAELTNALGLNRSWPVAVVGMGNLGRA
                     LAAYSGFATEGFYVEALFDHDPSVIGDLVGRHRIASMGELTAVVARLGIAIGVIATPA
                     NSAQDVADALVGAGVRAILNFAPVALALPEGVACRSVDLATELHVLAFLRQPERVGAD
                     VGRGQ"
     gene            4987..6663
                     /locus_tag="PLK64_06430"
     CDS             4987..6663
                     /locus_tag="PLK64_06430"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HPV79413.1"
                     /translation="MPEPVATRVTLGQAPVACGRVAFVGAGPGDPGLMTVRSRELLGM
                     ADVVVLDDLGEDFVRNFTCADVRVVRVSRHTGSTSGDGATASADGLTDESTDDLGEQL
                     VAEARRLGAGSLVVRLMYGDPMAFHGLAAEALSLRRAGVSFDVVPGVSPLTAAPAYAG
                     VPLTSGCATSVQVVHAGREPMSWGPAVEASVTVVVVGSGPRIAHTLEGLLTAGRDPLT
                     PVAVIEGGTTTLQASRVTTLAKASTLLADNGTGFPVVAVASQTVGLREALSWFETKAL
                     FGWDVLLPRTKEATDSVEERLSAYGARTTVVPTIAVELPRTPQQMERAVKGLVTGHYE
                     WVAFTSANTVRAVRTKVEEFGLDARAFAGVRIAAVGGLTVAALRDWGITADLVPSGEQ
                     SAAGLLEDWPEFDEDVDPMRSVLVPRADIATEALASGLADLGWEVDDVTAFRTVRAAP
                     PAATVREAIKGGAFDAVIFTSSSTVRNLVGIAGKPHPQTIVACVGPATAATAREHGLR
                     VDVVAEVPSSEALIDALAAHGTMLTVQAREAGLPPRRPSEPRPGSRRRSR"
     gene            complement(6753..7283)
                     /locus_tag="PLK64_06435"
     CDS             complement(6753..7283)
                     /locus_tag="PLK64_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018768789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HPV79414.1"
                     /translation="MTAFPGLTAGTWTVDPTHAEVGFVARHLMVAKVRGRFTEVSGTV
                     EVGETLAETSVRAVASAASVSTNQADRDGHLRSADFFDVETYPELTFVSTSVSSDSMT
                     GDLTIKGVTRSVTFDLEFDGVQADPWGNTKAGFTATTAINRSDWGLTWNAAIEGGGVL
                     VSDKIAITLEVELLKG"
     gene            7527..8027
                     /locus_tag="PLK64_06440"
     CDS             7527..8027
                     /locus_tag="PLK64_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPV79415.1"
                     /translation="MAAHSETNWLTEPEQRAWRAYLRGGRMLIEALDRGLAAHGLSLS
                     EYEIISMLSEEDTRQMRMSELADLVVQSRSRLTHTATRLEKRGWVVREPCQGDRRGVL
                     LTLTPTGAAKVEQIAPVHLDQVRAHLVDRLSAEQFATLGGAMENVMSGLDGFADSDGR
                     APNDRY"
     gene            8074..8721
                     /locus_tag="PLK64_06445"
     CDS             8074..8721
                     /locus_tag="PLK64_06445"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015770813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPV79416.1"
                     /translation="MSTPESTVVHLMRHGEVHNPDGILYGRLPGFRLSELGRAMATEV
                     AQHLAAHDIVHVVSSPLERALETARPTADSHGLGIHVDGRLIEAGNFFEGKVVGVGDG
                     VLKHPEYWSKLRDPFTPSWGEAYAAIASRMIAAVADARDAARGHEALLVSHQLPVWTA
                     RSRLEGRRLWHDPRKRQCALASLTSLHYLGNELVSVVYSEPAATLVRDAAKKAGA"
     gene            8718..9326
                     /locus_tag="PLK64_06450"
     CDS             8718..9326
                     /locus_tag="PLK64_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPV79417.1"
                     /translation="MTSTPVRWVAAGLALGAALSLAACQSDPNSLAAQAGSGSRAGYA
                     AGDGSIEQLPPASRGQALALSGTTVDGGTWSMDVQGAGKVVVINVWGSWCPPCVAETP
                     ALQGAWAAYEKAGKPVVFVGIDTMEAPETGLAFLRAKGVTFPSINDQANQGAPVLALQ
                     GKAPATPTTLVLDRQGRVAARVLGPTSEVTVRALVDAVLAEA"
     gene            9323..10096
                     /locus_tag="PLK64_06455"
     CDS             9323..10096
                     /locus_tag="PLK64_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HPV79418.1"
                     /translation="MTSLVLTDTSTIISGALPLAVLVAVAAGLVSFASPCVLPLVPGF
                     LGYVTGLSDVALADRRRSRMILGAALFVAGFSVVFIIGALAVSAAGAALAAHQSLLTR
                     IGGAIVIVLALVFLGVGAGMGSQREMRTHWRPAAGLAGAPLLGAAFAVGWSPCTGPTL
                     GAIYSLAAPLAGDAPVARGALLAAAYSVGLGVPFVLAAAGWSRAARANDWLRRHQRGV
                     QLAGGGLLLALGLLMVSGAWEGIMSWVQSHLIIGFETVL"
     gene            10162..>10792
                     /locus_tag="PLK64_06460"
     CDS             10162..>10792
                     /locus_tag="PLK64_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HPV79419.1"
                     /translation="MRQPTLGPLGWARWTWRNLTSMRTALFLLLLLSVAAVPGSIFPQ
                     RTIDPGRVGDYLVDNPTTGPWLDRLGFFNVYTSPWFSAIYLLLLVSLIGCIIPRSRLH
                     WHAIRSRPPRAPARLDRLAAHTTFTYAVPPAEALAAASTALRRRRYRVHSHDESSVSA
                     ETGYLRETGNLVFHLALCLVIVGVAVGHLWGWRGDVIVPEGQTFTSTFRS"
BASE COUNT         1483 a         3826 c         3701 g         1682 t
ORIGIN      
        1 acggtcaagc gttggtgccg ccgacagcct ccgaccctgc gcatgcgtac gcgcccgctc
       61 acccgggggt tagcgtggtc aagcggatca atggcgacga cgcagcgaca gctccaggag
      121 tctcggtttc agcgggctcg ctcatggcga tcaccttcga ggtgaccaac acgggcgacg
      181 tccgaatcga gccggtccag gtctctgact ccacggtcac cgacatccag tgtcccggca
      241 cggccctcga cccaggcgag gtgatgacgt gtagcgcgac ccttgcggcg ccggccttgg
      301 gcgtggagca caccaacacg gttaccgtga ccggcacccc ggtgctggcc gacgggacgc
      361 ccgctctcgg agatgacgga cagcccgcct cggcgccaac ggcaaccgac acggcatacg
      421 ccgtcatcgt cgcgccgttc ctgcccaaca cggggacgga cgtggcgcgg ttcctcgcaa
      481 tcgctctcgg cctgatggct gccggtggcc tgctgctcgc agcaacccgc cgccgccgcc
      541 gcgcgaccga ggagtgagca ccagccgggg gtagccccgt cacgactcgg cccgggcgac
      601 ccacatcgcc cgggccgcgg tcgtcaaccc aggcctggca gtgagcgggt aggctgcagg
      661 ccagccgaca atttcgactg agtagccaaa ggacgaccta tgggttctgt gatcaagaag
      721 cgccgcaagc ggatggccaa gaagaagcac cgcaaactgc tgcgcaagac gcgccaccag
      781 cgtcgcaacc gcaagtagtc gcaccggcat acccgcgggc ggccgcacct catccaggtg
      841 cggccgcccg cagccgttcg gtatgccgcc gcgcggctct ccggggcacc tcgggaccgc
      901 gccaggggct accggtgcgt aggattggcg gtggtggaca gggtcgccac cggatcgacc
      961 gacggaaggg agcaccgtgg ccaacgtcgt cctcatcacg ggcgtatccc ggtatctcgg
     1021 cgggcggttc gcgagcgcgc tgtccaagga cagcagcatc gagcggatcg tcgggatcga
     1081 tgtcgtgcca ccccctcact cgatcgggcg ggccgagttc gtcagggcgg acatccgtaa
     1141 cccgatgatc ggccggatca tcacccaggc caaggtcgac acggtcgtgc acatgaacgt
     1201 cctggccacg ccgacggcgg ccggagggcg ggtctcccag aaggagatca acgtcatcgg
     1261 gaccatgcag ttgctcgccg cctgccagaa ggccacctcg gtcaagcgac tggtggtcaa
     1321 gtcgaccgca gcgatctacg gcagttctcc acgcgaccca gcgctgttca ccgaggacac
     1381 cgggcccaag accatgccga cgagcgggtt tggcaaggac tccgtcgagg tcgagggcta
     1441 cgttcgtggc ttctcccgtc gtcgccccga tgccgagatc accctgttgc gcttcgccaa
     1501 cgtcatcggc ccaggcatca aaaccgcgat gaccgactac ttctcgttgc cgatcctgcc
     1561 ggtgcccatg ggcttcgacg cccggctgca gttcgtccac gaggacgatg cgacggccgc
     1621 catgttgttg gcggtccgcg ggccggcggt ccagatcgtc aacattgccg gcgacgggat
     1681 catcacggtc acccaggccg ccgcgatggt ggggcggccc gccgtgccca tcccgttcgg
     1741 ggcggcatac ctcgggtcgc tcgtgcgccg cgccgggctc gccgacttct ccaccgacca
     1801 actgtccttc ctctcctacg ggcgcggcct ggacacgacg aggatgcgcg aggtcatgaa
     1861 gttcaccccc cgtcacacca ctcgcgaagc gttcctcgac ttcgccgacc acgttccgcc
     1921 ggtgcttccg ggcgccgaca ccgccgcaag cctcttgggc cgggccgccg gtagcgccct
     1981 gcagtcggcc ggcggtctgg ccggccggat ccgtcaggtg gccggctgat gcccggcacg
     2041 tcgcgggacg ccttcgccgc gggcgccgcc cgggccgccg gagagcgccg caagcgcagt
     2101 agcaccccac tggtcccggc cgacggtatg ccgcccctgc ccaccgacga ggcgcgctcc
     2161 gcgccacacc ggcccgtccc ccaggtgact ccgaccgcat cggccaacgc gacgacggtc
     2221 gacgcggtgg ctcgtgaacg ccggccccgt cccgtcctcg ccgggctgac gaacgtcggt
     2281 gaggccaccg tcacccaact ccccacgacc cgagcgtctg accgggcggc cgggagcacc
     2341 cggctgacgc cggcgcgtcg caccggtcga cgcacccacc gcgagagcct gccgatcgcc
     2401 gagggcgccc tgacctcggc ggtgagcgcg ccggacacct cgatcacgcc ggagttgtcg
     2461 agcgtggctg ccgaccagcc ggctgcggcg accagggtca tcgagcccgc tgtgacggcc
     2521 gaggcggcgg gggagtcggc ggcgtcgacc gttcaggcgt ccacccaggc gtccccgcgc
     2581 gtgcgggggg accggaccgg cacggcatac cgctcccggg nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn caggcgtccc cgcgcgtgcg gggggaccgg accggcacgg
     2761 cataccgctc ccgggcgcgc accccggtcc aggcggagat ctctgaatcc ctgctcgcct
     2821 cggccctcac ccagcaggag ttgacgcccg acatcgagga catcgtcgcg gcgatcatcg
     2881 gcgtcctgcg caccgtcgcc gcggcctccg ggctcggcgc cgacgaggtc gagcaggcgg
     2941 tcgcccgcac cctggccttc ctgcgacgac ggctgaccgg cgactactcg gtcgatgact
     3001 tcggcctcga ctcggactac atcgagaaca tcttcctgcc cctcatgcgg gtgctctatc
     3061 ggcagtggtt ccgcgtcgaa gtccgcggca tcgagaacat cccggccgag ggctccgcgc
     3121 tcatcgtcgc caaccactca ggcaccatcg ccctggactc gcacatggtg caggtggcca
     3181 tccacgacga gcaccctgcg gcgcggcacc tgcggctgct cggggccgat ctggtcttcc
     3241 ggacgccaat catggggtca ctggcccgca aacagggcgc gaccctggcc gccaactccg
     3301 acgccgagcg actgttgtcg gccggcgagg tggtgggggt gttccccgag ggtttcaagg
     3361 gggtcggcaa gccgttcagc gagcgctaca agctccagcg cttcggtcgt ggcgggttcg
     3421 tcagcgctgc ggtccgcacc ggtgcaccga tcatcccgtg cagcatcatc ggggctgagg
     3481 agacctatcc gctgctgggc gacgtcaagg tgctcgcccg gctcttcggc gctccttact
     3541 tcccggtgac ccccacctgg ccgtggctgg gtccgttggg gctgttgccg ctgccgtcga
     3601 aatggatcat ggagttcggt gagccggtcg agacggcccg ctacggcgcg gctggcgccg
     3661 acgacccgat gctcgttttc gacctcaccg accaggtccg cgagaccatc cagcagaccc
     3721 tctactcgct gctcatgcag cgccgctcgg tcttctcctg acgggtcggc gacctcggtg
     3781 ttcgggcggc gcaagcagtc ggtccgggat gcgggcaccc cgcgcatcgt cctggtcgga
     3841 cgggtgggct gccatctgtg cgacgaggcc gaggcgatca tctcccgggt cgcggccgag
     3901 accggggcga cctgggtcaa ggtgtcggtc gacgacgacc cggccctgct ggcccggtgg
     3961 agcgaccagg tgcccgtcac gctcgtcgac ggccgacccc atgacttcta tcgagtgtcc
     4021 gaggctcgcc tgcgcgccgc cctgaagtcc tgatcgcgga caaacccgtt gggaggcaag
     4081 cgattccggg gggtgttcta ggtgacgttc cgcactttgt gcatttcttc acaaacccct
     4141 acattggagg ccagacccgc ggcgtcgcgc cctgctgcgc ccggaacgct gcgcaggaag
     4201 gagtcggtgg actgtggtcg gcacgctcgc ccctcgccgc gacatccctt cagcgaccgt
     4261 gtcccgcctc ccggtctacc tgcgtgcttt gcacgagttc gccacccgtg acgtcacccg
     4321 ggtttccagt gaggaacttg ccgctcaagc gggcgtgcga cccacccaac tgcgcaaaga
     4381 cctcagccac ctgggctcac acggtgtccg gggggttggc tacgacgtcg gacggctcac
     4441 ggctgagttg accaatgccc ttggcctcaa ccgctcctgg ccggtcgccg tcgtggggat
     4501 gggcaacctc ggccgcgccc tcgcggcata ctccgggttt gccaccgagg gcttctacgt
     4561 cgaggcgctg ttcgaccacg acccgtcggt catcggtgac ctagtcgggc gacaccgcat
     4621 cgcctccatg ggcgagttga cggctgtcgt cgctcgcctc gggatcgcca tcggcgtgat
     4681 cgccacgccc gccaactcgg cccaggacgt ggccgacgcc ctcgtcgggg cgggggtgcg
     4741 cgcgatcctc aacttcgcac ccgtggccct cgcgctgcct gagggggtgg cctgcaggtc
     4801 cgtggacctg gccaccgaat tgcatgtctt ggcatttctg cgccagcccg agcgggtcgg
     4861 cgccgacgtt gggaggggcc aatgaaggga cccgatctcg ccgggtgggg gcgttccaac
     4921 gagccgaggc tggctggcca agcgctaaac ggatctggat ccgcctctgc cgcaacggcg
     4981 ctggccgtgc ccgagccggt tgcgacccgg gtgacgctgg gtcaggcccc ggtcgcttgc
     5041 ggccgggtcg ccttcgtcgg tgccgggccc ggggaccccg gcctcatgac ggtgcgctcg
     5101 cgggagttgc tcgggatggc cgatgtcgtc gtgctcgacg acctcggcga agacttcgtc
     5161 cgcaacttca cctgcgccga tgtgcgagtg gtgcgggtgt cgcggcatac cggctcgacc
     5221 tcaggtgacg gcgcgaccgc gtccgccgac gggctcaccg acgagtccac cgacgatctc
     5281 ggcgagcagt tggtcgccga ggcgcgccga ctcggggccg gaagtctcgt cgtcaggctg
     5341 atgtacggcg accccatggc tttccacggt ttggccgccg aggccctctc gctgcgccgc
     5401 gcgggtgtct cctttgacgt cgtccccggg gtcagcccgc tgaccgccgc tcccgcttat
     5461 gccggcgtcc cgctcacctc cggctgcgcc acctcggtgc aggtcgtcca cgccggccgg
     5521 gagccgatgt catggggccc tgccgtcgag gcctcagtga ccgtcgtcgt cgtcggctcg
     5581 gggccgcgga tcgcccacac cctcgagggc ctgctgacag ccggccgcga cccgctcacc
     5641 ccggtcgccg tcatcgaagg tgggacgacg accctgcagg ccagccgggt caccaccttg
     5701 gccaaggcgt caaccctgtt ggcggacaac ggcaccggct tccccgtcgt tgcggtggcc
     5761 agccagaccg tcggcctgcg cgaagccttg tcgtggttcg agaccaaggc gttgttcggc
     5821 tgggatgtcc tgctgccccg caccaaggaa gcgaccgact cggtcgaaga gcggctgtcg
     5881 gcctacgggg cgcgcaccac cgtcgttcca acgatcgccg tcgagctgcc tcgcactccg
     5941 cagcagatgg agcgcgcggt caagggcctg gtgacggggc actacgagtg ggtggccttc
     6001 acgtcggcca acacggttcg cgcggtgcgg accaaggtcg aagagttcgg cctggacgcg
     6061 cgcgccttcg ctggcgtccg gatcgccgcc gtcggcgggc tcacggttgc ggcgctgcgc
     6121 gactggggca tcaccgccga tctcgtcccc agcggcgagc agtcggcggc cgggctgctc
     6181 gaggactggc cggaattcga cgaagacgtc gacccgatgc gctcggtgct cgtgccgcgc
     6241 gccgacatcg ccaccgaggc gctcgcctcc ggcctggccg atctgggctg ggaggtcgac
     6301 gacgtcacgg ccttccggac ggtgcgggcg gctccgccgg ccgccaccgt gcgcgaagcg
     6361 atcaagggcg gggccttcga cgcggtgatc ttcacgtcga gttccaccgt gcgcaacctc
     6421 gtcggcatcg ctggcaagcc ccacccgcaa accatcgtgg cctgcgtcgg tccggcgacc
     6481 gcagccacgg cccgcgagca cggcctgcgg gtcgatgtcg ttgccgaggt cccgtcatcc
     6541 gaggccctca tcgacgcgct cgcggcgcac ggcaccatgc tcaccgtgca ggcgcgggag
     6601 gccgggttgc cgcctcgtcg gcccagcgaa ccgcgccccg ggtcccggcg ccgctcgcgc
     6661 tgagccccca cacgcgaacg ccccggtatg ccgcgtgggc ggcataccgg ggcgtgtgtg
     6721 gtgagccctg gggtgggttc gaccgcggtg ggtcagccct tgagcagttc gacctcgagc
     6781 gtgatggcga tcttgtcgga caccaggacg ccgccgccct cgatggccgc gttccaggtc
     6841 aggccccagt cggaccggtt gatcgcggtc gtcgcggtga agccggcctt ggtgttgccc
     6901 cacgggtcgg cctggacacc gtcgaactcg aggtcgaagg tgaccgagcg ggtgacaccc
     6961 ttgatggtca ggtcaccggt catcgagtcg gaggacacgg aggtggagac gaaggtgagc
     7021 tcggggtagg tctcgacgtc gaagaagtcg gcgctgcgca ggtgaccgtc acggtcggcc
     7081 tggttggtgg agaccgacgc ggcagaggcg acggcgcgca ccgaggtctc ggcgagggtc
     7141 tcgccgacct cgacggtgcc actgacctca gtgaagcggc cgcgaacctt ggccaccatg
     7201 aggtggcggg cgacgaaacc cacctccgcg tgggtggggt cgacggtcca cgtgccggcg
     7261 gtcagtccgg ggaaagcagt catgatggtc ctttcgggtg ggggccgtcg ggcggccgtt
     7321 gataaggcaa ataagattga actctcaact attcccagaa gcctgcgatg tctcaccatc
     7381 tggacctgac ctggtcggac cccgcggagt gacgttatcg gcgtctgtcc ggcggctggc
     7441 ctccgggccc tatgctgggc atacggcggg ccggggtcgc gactcggggc tcgctgcagg
     7501 gatcccgtcg atatgaatgc gcactgatgg ccgctcacag cgaaaccaac tggctcaccg
     7561 agcccgaaca acgggcctgg cgagcctact tgcgcggcgg tcgcatgctc atcgaagccc
     7621 tcgatcgggg gctggcggcg cacgggctga gcctgtcgga atacgaaatc atctcgatgc
     7681 tgtccgagga agacacccgg cagatgcgga tgtccgagct cgccgatctg gtcgtgcagt
     7741 cccgcagccg actcacccac accgcgaccc ggttggagaa gcggggctgg gtcgtgcgcg
     7801 agccgtgcca gggtgaccga cggggagtgc tgctcacgct cacgcccacc ggtgccgcca
     7861 aggtggagca gatcgctccc gtccacctcg accaggttcg agcccacctg gtcgaccgat
     7921 tgagcgccga gcagttcgcg acattgggcg gagcgatgga gaacgtcatg tcgggcctgg
     7981 acgggtttgc ggacagcgac ggccgggcgc ccaacgaccg ctactgattg gtgggccgct
     8041 gcggcccaca gtgagtgctg gaagaatggg gccatgtcca ctccggagtc gaccgttgtc
     8101 cacctcatgc gccacggcga ggttcacaac cccgacggga ttctttacgg ccggctgccc
     8161 ggcttccggc tgtccgagtt gggtcgggcc atggcgacgg aggttgcgca acacctggcc
     8221 gcccacgaca tcgtccacgt cgtgtcctcg ccgttggagc gcgcgctcga gaccgcacgg
     8281 ccgacggccg acagtcacgg gctgggcatc cacgtcgatg gtcgcctcat cgaggcgggc
     8341 aacttcttcg agggcaaggt cgtcggggtg ggtgacgggg tgctcaagca cccggagtac
     8401 tggtccaagc tgcgcgaccc cttcaccccc agttggggcg aggcgtatgc cgcgattgct
     8461 tcccggatga tcgctgcggt cgccgacgcc cgtgacgccg cccgtgggca cgaagcgctg
     8521 ctcgtcagcc accagcttcc cgtgtggacg gctcgctccc gcctggaagg gcgccggcta
     8581 tggcacgacc cgcgcaagcg ccagtgcgcc ctcgcctcgc tgacgtcgct gcactacctc
     8641 ggcaacgagc tcgtctcggt cgtctacagc gagcccgccg ccaccctggt gcgcgatgct
     8701 gcgaagaagg ctggcgcgtg acctcgactc cggttcggtg ggtcgctgcg gggcttgcgt
     8761 tgggggccgc cctctcgctg gcggcctgcc agagcgaccc caactccctc gccgcccagg
     8821 ctggttcggg cagccgcgcg gggtatgccg cgggcgacgg ctcgatcgag caactgcccc
     8881 cggcctcgcg cgggcaggct ctcgcgctct ccgggacgac tgtcgacgga ggcacctggt
     8941 cgatggacgt gcagggcgct ggcaaggtcg tcgtcatcaa cgtctggggg tcctggtgcc
     9001 caccgtgcgt ggccgagacg ccggccctgc aaggtgcgtg ggcggcatac gagaaggccg
     9061 gtaagccggt cgtcttcgtg ggaatcgaca cgatggaggc cccagagacg gggctggcct
     9121 tcctgcgggc caagggcgtg accttcccgt cgatcaacga ccaggccaac cagggtgccc
     9181 ccgtcctcgc gttgcagggc aaggcgcccg ccacgccgac gactctcgtg ctcgaccgtc
     9241 aggggcgggt ggccgcccgg gtgctcggcc cgaccagcga ggtcaccgtc cgggcgctcg
     9301 tcgacgccgt gcttgccgag gcgtgacctc gctcgtgctc acggacacct cgacgatcat
     9361 ctccggcgcg cttccgctgg cggtgctcgt cgcggtggcg gccgggctgg tgtccttcgc
     9421 ctcgccgtgc gtgctgccgc tggtgccggg gttcctcggc tatgtcaccg gcctgtccga
     9481 cgtggccctg gccgaccggc ggcgctcgcg gatgatcctc ggggctgcct tgttcgtcgc
     9541 ggggttcagc gtcgtcttca ttatcggagc cctggccgtg tcggccgccg gggcggccct
     9601 cgcggcgcac cagagcctgc tgactcgcat cggcggggcc atcgtcatcg tcctggcgtt
     9661 ggtcttcctc ggcgtcggtg ccggcatggg cagccaacgc gagatgcgca cgcactggcg
     9721 cccggcggcc ggactggcgg gggcaccgtt gctcggcgcc gcgttcgccg tcggctggag
     9781 cccgtgcacc ggccccaccc tcggggcgat ctactccctc gcggcgcccc tggccggtga
     9841 cgccccagtc gcgcgcggag ccctgctggc ggcggcatac agcgtgggtt tgggggtgcc
     9901 cttcgtcctg gctgccgcgg ggtggtcacg cgcagcacgg gccaacgact ggctgcgccg
     9961 ccaccagcgc ggcgtgcaac tggccggcgg cgggctgctg ctcgcgctgg gcctgttgat
    10021 ggtcagtggc gcctgggaag gcatcatgtc gtgggtgcag tcccacctga tcatcggatt
    10081 cgagaccgtg ctgtgagcgc tgacgactca accgcaggca gcgcgaccac cgaggcgacg
    10141 accgaggcga ccaccgaggc gatccggcag ccgaccctcg gtccgctggg ttgggcgcgg
    10201 tggacgtggc gcaacctcac ctcgatgcgc acggcgctct tcctgttgtt gctgctgtcc
    10261 gtcgccgccg tgcccggctc gatcttcccg cagcgcacca tcgacccggg gcgggtcggg
    10321 gactacctcg tcgacaaccc gaccaccggc ccctggctcg accggctggg gttcttcaac
    10381 gtctacacct cgccgtggtt ctcggccatc tacctgctgt tactcgtgtc gctcatcggc
    10441 tgcatcatcc cccggagccg gctgcactgg cacgcgatcc gctcgcggcc accccgcgcc
    10501 cccgctcgcc tggatcgcct ggcggcccac acgaccttca cgtatgccgt cccgcccgcc
    10561 gaggcgctcg cggcggcctc gaccgcgttg cggcgccgcc gctaccgcgt ccactcgcac
    10621 gacgagtcgt cggtcagcgc cgagaccgga tatctgcgcg agaccggcaa cctcgtcttc
    10681 cacctggccc tctgtttggt catcgtcggg gtcgcggttg gacacctgtg gggctggcgc
    10741 ggcgacgtca tcgtccctga gggccagacc ttcacctcga cgttccgcag ct
//
LOCUS       DAOMZY010000084        10781 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5723_length_10781_cov_6.460004, whole genome shotgun sequence.
ACCESSION   DAOMZY010000084 DAOMZY010000000
VERSION     DAOMZY010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 10781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10781
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5723_length_10781_cov_6.460004"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..1365
                     /locus_tag="PLK64_06465"
     CDS             193..1365
                     /locus_tag="PLK64_06465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPV79420.1"
                     /translation="MDFTLTDEQRMLVKTIRRFVYDELIPLEDDIEATGALDPAAARS
                     IFEKSRDLGFYAMNIPEEFGGGGLSALETMLVEEQFGHCKDILIRRAFGNVYEVLLEG
                     DAAQRERWLLPTVSGERTCSIAITEPGAGSDAASITTRAERTATGWTLNGGKYFVSDG
                     LYSDFFIVSAVTDAAARPRHISLFLVDKNLPGVRVGQDQKMMGLAGSSHIELFFDDVH
                     LGPETLIGGEGNGLRLALTALGRVRLAQVGARAVGKASRLLGYMADYAGQREQFGSPI
                     GSFQMVQAMLADSAIEINRSRLLLHRAAAALDAGEDYRSWISMVKVDAAEMLGRVADR
                     AVQVFGGTGYTHGFPVERMYRDARIFRIFDGTSEIHRTVVAAGVLKGGAKTWDIGA"
     gene            1382..2911
                     /locus_tag="PLK64_06470"
     CDS             1382..2911
                     /locus_tag="PLK64_06470"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HPV79421.1"
                     /translation="MPRNKFRTVAEAVALVSDGATVGLVGGGSGLQEAGALFAEVERR
                     FLETGHPAGLTLVHALGIGDKAERGLNRFAHRGLVSAVIGGHWVWSPRMQALAADNEI
                     EAYVLPGGVVGQLYREIGAGRPGLFTHVGLGTFVDPRHGGGRMNAAATRTLADVLEID
                     GREVMRYRPFPVDIALLRGSYADADGNISFAQECANLDMFDLALAAHNSGGKVIVQVR
                     TAVDRGTIPARDVHIPGAWVDAVVVDPTQSMTYDIVYDPTMSGEVRGPVPPRPPEPFT
                     ERVAVARRAALELRDDAVVNYGFGMPDLVARIVADRGDTERYYQTIEHGVYGGDLLTG
                     SLFGFGRNATAYIHGPSQFDFYAGGGLDLAFLGFGEFDAQGNVNVSMLGGVTVGPGGF
                     MDIAANARKVVFCGTFEAKGLRVRPVDGRLVVDQPGSVPKVVAQVDQVTFSGPQAVAR
                     GQEVLYVTERAVFRLTPEGIVLAEVAPGIDVQTQVLDRMGFAPLLPQPPAVIDGAIFR
                     E"
     gene            complement(3000..3692)
                     /locus_tag="PLK64_06475"
     CDS             complement(3000..3692)
                     /locus_tag="PLK64_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79422.1"
                     /translation="MADDDLPETDDDAPVLRAVPDSAVGYRLVLPPGWLRLDARADAG
                     RARLDAELDRVLPKTYQPTLAPVVAQARAQAHSALHQARDAGALDLYLPLGGVRGIPI
                     PASFLVAVRRFAAAPPPTLAAQGPEALAVALIANLAATMPDAKTRMMAAGPAVRSTRP
                     VTGAASDTSPAMTGVRVDYIWPVPGDAALWVMASFTTMRTPELPASITDQLVALFDAI
                     MSTWRWDPGLAL"
     gene            complement(3685..4356)
                     /locus_tag="PLK64_06480"
     CDS             complement(3685..4356)
                     /locus_tag="PLK64_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79423.1"
                     /translation="MRRGGIPAGGEPEPQVGALFAVTYDSRRWIPAPPSRRIPSDGAD
                     HFDRWVTLAGSRFLTPADDPAGPKAAAIGRILRTLGSLDTPSWDPFWLQKWIHWPSLA
                     DLPTMVIVSGFPATGGGTTDELRGYCGEGVPGVLEEPIIEPVSSTRLGSGLRSLAYTA
                     EMADGLAEVHATLAYVWRIRLLDDGRPTLGIVRLWASGSPRQIIAAEPDIHDLALTLR
                     METSG"
     gene            complement(4796..5275)
                     /locus_tag="PLK64_06485"
     CDS             complement(4796..5275)
                     /locus_tag="PLK64_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79424.1"
                     /translation="MASFRDIPGLEPPDAPLWVDHVLRWCVVATFLGYFAFIFRWIDS
                     ATWWGPWLPALPGVLGPLVVLPWWWPPERRPADALRLIVAGLIPAMFPAFALLSLPIA
                     LAGLLIVGLVVLAPRLATEGMRSVGAPSGRIRLWTHSRYLRKSAATSRARRRARGKQ"
     gene            complement(5349..5555)
                     /locus_tag="PLK64_06490"
     CDS             complement(5349..5555)
                     /locus_tag="PLK64_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79425.1"
                     /translation="MATFRGIPGLEPPDALRLIVAGLIPAGAPAFAPLSLPIALAVLL
                     SMFTIRRFRRQALANYRASRRERR"
     gene            complement(5645..7048)
                     /locus_tag="PLK64_06495"
     CDS             complement(5645..7048)
                     /locus_tag="PLK64_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79426.1"
                     /translation="MSSIARSTHWPPLCATDPTPGDDEIVRSAGRKFQGVADEIERQI
                     AELGKIADGSSSLQGQYVATLQEACEAVKGDLGKIKRRYREVGGLLVGWAPELVDFQR
                     EAERLLDDAQAAKRGLDGLVTITVPYDQMTDEQKADAQQRQRRASELNGDLNEARRKV
                     HDLQDRRDRAAKRVADKIEEASADDLTDSWWDNVKDWFAAHKELIDGICKVLGVIALV
                     ALVVCLVVPGLNIAAGAALATAATYVGIGATSASLLLHTGQAATGTGTWLDVGLDAFS
                     LATFGAGRFLSVGAREAAGAVRVEAATVAGERAAAPILAQAAAAVDDLAKARAAIPWW
                     KIWQWGKAARLEDAMALVKQAGQTEAAAARVAAEEARLAEQVTIKVRNVLRYGDGDLA
                     KIGMQLREDAKYLKGLTESVQLVDEAVGATGKAAAMFLTSGGTTVVSAGSSFYSPEWW
                     NTMRETFVWEVGSLHRP"
     gene            complement(7045..7359)
                     /locus_tag="PLK64_06500"
     CDS             complement(7045..7359)
                     /locus_tag="PLK64_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79427.1"
                     /translation="MTIEVNYAVLTDSANALRRIAGEFNDAVTIKDHYGKAMGAGSIE
                     RALGTFGGNWDRHAKDLVERLQKTESTFRSVAESFREQDRIGAGSIQETGSAPSGGNT
                     PR"
     gene            complement(7433..7990)
                     /gene="ispF"
                     /locus_tag="PLK64_06505"
     CDS             complement(7433..7990)
                     /gene="ispF"
                     /locus_tag="PLK64_06505"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016996018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPV79428.1"
                     /translation="MSLEPVVPVIPRVGVGVDVHAFAPDGSERELWVAGLYWPGERGL
                     DGHSDADVACHAACDALFTAAGIGDLGTHFGTDRPEYAGASGRALLAEAARLVREAGF
                     EIGNVAVQVIGNRPKIGRHRELAEVALSAACGASVSLSGTSTDGLGLTGRGEGIAAIA
                     TAIVYAVRAPTPPPTSPRSDPPTGI"
     gene            complement(8041..8829)
                     /locus_tag="PLK64_06510"
     CDS             complement(8041..8829)
                     /locus_tag="PLK64_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPV79429.1"
                     /translation="MAPVAVITGGGTGIGAATATRLAQDGFDVVVCGRRPGPLEETAA
                     AIRAAYPSVTARAIVLDVTDAASVAAAAAEIGPCDVLVNNAGGAIGSERVEDGKPDEW
                     RAMYESNVIGVLQVTQALLPALRRSPTGGTIVVVSSIAGLLVYEGGGGYTAAKHGATA
                     VVETLRLELAGEPIRVIEIDPGMVWTDGFSLTRFHGDQGKADAVYAGVDRPLVAADVA
                     ECVAFCVRLPQHVNVDRLIVKPVAQAALHKVIRTPIDWDHSATS"
     gene            complement(8841..9524)
                     /gene="ispD"
                     /locus_tag="PLK64_06515"
     CDS             complement(8841..9524)
                     /gene="ispD"
                     /locus_tag="PLK64_06515"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HPV79430.1"
                     /translation="MSVGVVIVAAGSGSRLAASVPKAFVLVGGRPLLEYAVRAALSAR
                     PAAVVVAAPASHVDEALDLVEPLSREHGIPVQVVPGGAERGDSVLAGVRWLPEACDVV
                     LVHDAARAFTPSAVFERVAAAVTEAAPAVVPVMPVVDTVKVVDAEGFVVSTPDRAALR
                     IVQTPQGFRRDVLLAAHAAHGSLATDDAGLVELLGLRVGTVPGDPAAYKITSPADLAH
                     AERLVDHRG"
     gene            complement(9551..10033)
                     /locus_tag="PLK64_06520"
     CDS             complement(9551..10033)
                     /locus_tag="PLK64_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HPV79431.1"
                     /translation="MVFKVGETVVYPHHGAALIQEINTRTIKGVERLYLTLKVAQGDL
                     TIQVPADSCEDIGVRDVVGQEGLDRVFQVLRAAYTEEPTNWSRRYKANLEKLASGDVI
                     KVAEVVRDLWRRDKDRGLSAGEKRMLAKARQILVSELALAEKTNEEKAESILDEVLAS
                     "
     gene            10310..>10781
                     /locus_tag="PLK64_06525"
     CDS             10310..>10781
                     /locus_tag="PLK64_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79432.1"
                     /translation="MTRLHRALAVTGAAAATAALSACSFFSPVQTDNKYIPADGVPVQ
                     LGVIAGRNLAIVADAAGGPGALTGAVLNQGDAEAVVGFLTREESEAGASVNTRLSLGG
                     RESKPISGVVFEKVTSAPGTMTDIYLVTTEGKQLVSVPVIVPGAPVTFSNTTPLI"
BASE COUNT         1680 a         3884 c         3607 g         1610 t
ORIGIN      
        1 gccctgctgg ccgccggggc ggcccgggtg ccccgcaagc acctgacgat gacgcgccga
       61 ccagccggct gacccgcagc ctagaactcc cgcgtgaccc acctcacgtg cgtatgtggc
      121 ggagcggcat actctgcgta tattggacga actagttagt tatcttctct cctcctcccc
      181 cggacggcag ccatggactt cacgctcacc gacgagcagc ggatgctcgt caagacgatc
      241 cgtcgtttcg tctacgacga gctgatcccg ctcgaggacg acatcgaggc gaccggcgcc
      301 ctcgaccccg ccgcggcccg gtcgatcttc gagaagtccc gtgatctcgg cttctacgcg
      361 atgaacattc ctgaggagtt cggcggcggc gggctgtcag cccttgagac catgctcgtc
      421 gaagagcagt tcggccattg caaggacatc ctcatccggc gggccttcgg caacgtctac
      481 gaggtgctcc tcgaaggcga cgccgcgcag cgtgagcgtt ggctgcttcc caccgtgagc
      541 ggcgagcgca cctgttcgat cgccatcacc gagccggggg cgggctccga cgccgcctcc
      601 atcacaaccc gcgccgaacg cacggcgacc ggctggaccc tcaacggcgg caagtacttc
      661 gtcagcgacg gcctctactc cgacttcttc atcgtctcgg cggtcacgga cgccgccgcc
      721 cgcccgcgcc acatctcgct cttcctcgtc gacaagaacc tgcccggcgt ccgggttggt
      781 caggaccaga agatgatggg cctggccggg tcctcccaca tcgagttgtt cttcgacgac
      841 gtccacctcg ggcccgagac cctcatcggc ggcgagggca acggcctgcg cctggccctc
      901 accgcactgg gtcgcgtccg gctggcccag gtcggtgcgc gggcggtcgg caaggcgtcg
      961 cggctgctcg gttacatggc tgactacgcc ggccaacgtg agcagttcgg gtcgccgatc
     1021 gggagtttcc agatggtcca ggcgatgctc gccgacagcg ccatcgagat caaccggtcg
     1081 cggttgctgc tgcaccgcgc ggctgccgca ctcgacgcgg gcgaggacta ccgctcgtgg
     1141 atctccatgg tcaaggtcga cgcggccgag atgctcggcc gggtcgccga ccgagctgtg
     1201 caggtcttcg gcggcacggg ctacacccac ggcttccccg tcgagcggat gtaccgcgac
     1261 gcccgcatct tccgcatctt cgacggcacc tccgagatcc accgcaccgt cgtcgccgcc
     1321 ggcgtgctca aaggcggcgc gaagacctgg gatatcgggg catagggaaa ggccagttgg
     1381 tatgccgcgc aacaagttcc gcacggtcgc cgaggccgtg gcactcgtct ccgacggcgc
     1441 gaccgtcggg ctcgtcgggg gcggcagcgg tctgcaagag gccggtgccc tgttcgccga
     1501 ggtcgagcgt cggttcctcg agacgggtca ccccgccggg ctgaccctcg tgcacgccct
     1561 cggcatcggc gacaaggctg agcgcggcct caaccggttc gcccatcgcg gcctggtctc
     1621 agcggttatc gggggccact gggtgtggtc gccgaggatg caggccctgg ccgccgacaa
     1681 cgagatcgag gcctacgtcc tgcccggtgg tgtcgtcggc cagctctatc gcgagatcgg
     1741 cgccgggcgg ccaggcctgt tcacccacgt cgggctgggc accttcgtcg acccccggca
     1801 tggcggtggc cggatgaacg ccgcggctac gcgcacgctc gccgacgtcc tggagatcga
     1861 cggccgcgag gtcatgcgct accgcccgtt ccccgtcgac atcgctctcc tgcgcgggtc
     1921 gtatgccgat gccgacggca acatctcctt cgcccaggag tgcgccaacc tcgacatgtt
     1981 cgacctcgcc ctggctgccc acaacagcgg cggcaaggtc atcgtccagg tccgcaccgc
     2041 ggtcgaccgc ggcaccatcc cagcccgcga cgtccacatc cccggcgcct gggtggatgc
     2101 ggtcgttgtc gatcccactc agtcgatgac ctacgacatc gtctacgacc cgaccatgtc
     2161 cggcgaggtc cgcggccccg tgccgccgcg cccgcccgag ccgttcaccg agcgagtcgc
     2221 cgtcgcccgc cgggccgcct tggaactgcg cgacgacgcg gtggtcaact acggcttcgg
     2281 tatgccggac ctcgtcgccc gcatcgtcgc cgaccgaggc gacaccgagc gctactacca
     2341 aaccatcgag catggcgtgt atggcggcga cctgctcacc gggtcgctct tcggcttcgg
     2401 tcgcaacgcc acggcataca tccatggtcc gtcccagttc gacttctatg ccggcggcgg
     2461 gctggatctg gccttcctcg gcttcggtga gttcgatgcg caggggaacg tcaacgtctc
     2521 gatgctcggc ggagtgacgg tcgggccggg cggcttcatg gacatcgcgg cgaatgcgcg
     2581 caaggtggtg ttctgcggga ccttcgaggc caagggtttg cgggtccggc cggtcgacgg
     2641 tcgactcgtg gtcgatcagc ccgggtcggt gcccaaggtg gtcgcccagg tcgatcaggt
     2701 gaccttctcg ggcccgcagg cggtggctcg cggccaggag gtgctctatg tgaccgagcg
     2761 tgcggtcttc cggctcaccc ctgagggcat cgtgctggct gaggtcgcgc ctgggatcga
     2821 cgtccagacc caggtgctcg accggatggg tttcgctccg ctgctgccac aaccgcccgc
     2881 ggtcatcgac ggagccatct tccgcgagtg actctcgccc ccctctcttg ccgggtcgag
     2941 gtattccgcc atcgcctggt gatggcggaa tacctcgacc cggcagaggg ggcagcgggt
     3001 cagagggcca ggcctgggtc ccaccgccac gttgacatga tcgcgtcgaa aagggcgacc
     3061 aactgatcgg tgatgctcgc cggcaactcc ggcgtccgca tcgtcgtgaa cgacgccatc
     3121 acccacagcg cggcatcgcc cggcaccggc cagatgtaat cgacccgcac ccccgtcatg
     3181 gccggcgaag tgtcggaagc ggccccagtg accggccggg tcgagcgcac cgccggcccc
     3241 gccgccatca tccgggtctt cgcgtcgggc atggtggcgg ccaggttggc aatgagcgcg
     3301 acggcgagcg cttccgggcc ctgggcggcc agggtgggcg ggggcgccgc cgcgaagcgc
     3361 cggaccgcga caaggaacga cgccgggatc ggtatgccgc gcacgccccc cagcggcaag
     3421 tagagatcca aggccccggc gtcacgggct tggtgcagcg ccgaatgggc ctgcgcccga
     3481 gcctgggcca cgaccggcgc gagcgtcggt tggtaggtct tgggcaggac ccggtcaagc
     3541 tcggcgtcca gccgggcgcg cccggcgtcg gcccgggcgt ccagtcgcag ccatccgggc
     3601 ggcaggacga ggcgatagcc caccgcgctg tcgggcaccg cccgcaggac gggggcgtcg
     3661 tcgtcggtct ctgggaggtc gtcgtcagcc actggtctcc atccgaaggg tcagggcaag
     3721 gtcatggatg tcgggctcag ccgcgatgat ctggcgcggc gacccgctgg cccacagccg
     3781 cacgatgcct agcgtcggcc gcccgtcgtc gagcagccgg atccgccaga cgtaggccag
     3841 cgtcgcgtgg acctcggcca gcccatcggc catctccgcc gtgtaggcca ggctgcgcag
     3901 cccggacccg agtcttgtcg aggacaccgg ctcgatgatc ggctcctcca acacgccggg
     3961 cacgccctcg ccgcagtagc cccgcagctc atcggtcgtc ccacctccgg tcgccgggaa
     4021 cccactgacg atgaccatcg tcggcaggtc cgccaggctc ggccagtgga tccacttctg
     4081 caaccagaac gggtcccacg aaggtgtatc gaggctgccg agggtgcgca ggatccgccc
     4141 gatcgccgct gccttggggc ccgcggggtc gtcggcggga gtgaggaacc ggctgccggc
     4201 cagcgtgacc cagcggtcga agtggtccgc gccgtcgctg gggatccgac gactcggcgg
     4261 ggccgggatc cagcgccgag agtcgtaagt gacggcgaag agcgcgccga cctggggctc
     4321 gggctcccca cccgcaggta tgccgccccg cctcaccgga gcttccgcct gttggccctg
     4381 tctgcggcca ggatcagcgc gatgacaccg aggaccccgg cgactacgca gacccgaaag
     4441 acatgctcga cccaccgttg cgcaccgggg ggatctgcac ccggtatgcc gtgaaaggtc
     4501 gccacggcgt cagcctaacg cccagcggct cccacccctt ggcgcgcgaa cccgtgccgc
     4561 ccagcgcctt cctgctgtcg cctacgcaca acatcgtcaa tgttctgcac actcaccgaa
     4621 acatcgtcga gcaaacgaaa gttcgtcgac gttgccgggg ccgagtccga ctacaggtca
     4681 cgcaccgaga agtcgccgat gaagtcgccg ttactgctgt cccaacgaca gtcacgacga
     4741 cttcatgggc gccttcatgc gcgcagcgaa cgacatcgag gtgctcgagt cccgctcatt
     4801 gctttccgcg agcgcgccga cgcgccctgc tggtggcggc cgacttgcgc agatatcggg
     4861 agtgggtcca gagccggatc ctcccgctcg gcgccccgac agagcgcatc ccctcggtcg
     4921 ccaggcgcgg cgcgagcacg accagcccga cgatgaggag cccggcgagg gcgatcggca
     4981 gcgacagcag ggcgaatgcg gggaacatgg ccggaatcaa cccagccacg atcagcctga
     5041 gcgcgtcagc ggggcgccgc tccggtggcc accaccacgg caacaccacc agcggaccga
     5101 ggaccccggg cagcgccggc aaccaggggc cccaccacgt ggccgagtcg atccagcgga
     5161 agataaaggc gaagtagccc aggaaggtcg cgacgacgca ccaccgcagg acatggtcga
     5221 cccacaacgg ggcatcaggc ggttcaaggc ccggtatgtc gcggaagctc gccacggact
     5281 caggctaacg cccggcagct cccaccacct gatggcaccg actgggcggg cacagagccg
     5341 cggcccactc accgacgctc tcgccgggaa gcccggtagt tggccagggc ctggcgacga
     5401 aagcgccgaa tggtgaacat ggacaggagc acggcaaggg cgatcggcag cgacagcggg
     5461 gcgaatgcgg gagccccggc cggaatgagc ccggccacga tcagcctcag cgcgtcaggc
     5521 ggttcaagcc caggtatgcc gcggaaggtc gccactcaga cagcctaggc ggatacgtct
     5581 gcccatcccg agacctgagt ctcgatctga ccgtggacgg tgccgttggc ccgcctccga
     5641 cgccctaggg ccggtggaga ctgcccacct cccaaacgaa tgtctctctc atcgtgttcc
     5701 accactcagg ggagtagaag ctactcccgg ccgagaccac ggtggtgcct ccgctggtca
     5761 ggaacatcgc cgctgcctta ccggtcgcac cgaccgcctc gtcaacgagt tgaaccgact
     5821 ccgtcaggcc cttcaaatac tttgcgtcct cgcgaagttg catgccgatc ttcgccaaat
     5881 ctccgtcccc gtagcgaagg acgttgcgga ccttgatagt cacctgctcg gcaagcctgg
     5941 cctcctcggc ggcgacacgc gcggcggcgg cctctgtttg accggcctgc ttcacgaggg
     6001 ccatcgcgtc ctcaagcctc gcagccttgc cccattgcca gatcttccac caagggatcg
     6061 ccgcgcgagc cttagccaag tcgtccaccg cggcagcggc ctgcgcgagg atgggagcag
     6121 cagcccgctc gccggccacc gtcgctgcct cgacccggac cgcgccggcc gcctcgcgag
     6181 ccccgacgct gaggaaccgg cccgcgccga aggttgccag cgagaaggcg tccaggccga
     6241 catcgagcca cgtccccgtc ccggtcgccg cttggccggt atgcaagagc aacgaggccg
     6301 acgtggcacc gatgccgaca taggtcgccg cggtggcgag cgccgcgccc gcggcgatgt
     6361 tgagccccgg gacaaccaga cagaccacga gcgcgaccag cgcgatgacg ccgaggacct
     6421 tgcagatccc gtcgatgagc tctttgtgag cggcaaacca gtccttgacg ttgtcccacc
     6481 acgagtcggt gaggtcgtcg gcgctggctt cctcgatctt gtcggcgacc cgcttggcgg
     6541 cgcgatctcg gcggtcctgc aggtcgtgca ccttgcgccg ggcctcgttg aggtcgccgt
     6601 tcaactcgct cgcccgacgt tggcgttgct gcgcgtcggc cttctgctcg tcggtcattt
     6661 ggtcataggg gaccgtgatg gtgacgagcc cgtcgagccc ccgtttggcc gcctgagcgt
     6721 cgtcgagcaa ccgctcggcc tcgcgctgga agtccaccag ctctggcgcc caaccgacga
     6781 gcagcccacc cacctcgcga tagcgacgct tgatcttgcc cagatcgccc ttcacggcct
     6841 cgcaggcctc ctgcagggtg gcgacatact gcccctgcaa ggacgacgag ccatccgcga
     6901 tcttgccgag ctcagcgatc tggcgctcga tctcgtcggc gacaccctgg aacttgcgcc
     6961 ccgccgaccg gacgatttcg tcgtcgcccg gcgtcgggtc agtggcgcac agcggtggcc
     7021 agtgtgtcga tctcgcgatg ctgctcatcg aggagtgttc cccccgctgg gggccgagcc
     7081 ggtctcctgg atcgagccgg cgccaatccg atcctgctca cggaacgact cagcgaccga
     7141 acggaaggtc gactcggtct tctgcagacg ctcgaccagg tccttcgcgt gccggtccca
     7201 gttgcccccg aaggtcccga gcgcccgctc gatcgagccc gcccccatgg ccttgccgta
     7261 gtggtccttg atggtcacgg cgtcgttgaa ctcaccggcg atccgccgca gcgcgttggc
     7321 gctgtcggtg agcacggcgt agttgacctc gatcgtcacc agagaccttt ctgtctttcc
     7381 ttcgtccgga gtgtttcacc gccgccagcg gctcaccagt ccgtactcgt actcagatgc
     7441 cggtgggcgg gtccgagcgc ggggacgtcg gcggaggcgt cggcgcgcgc acggcataca
     7501 cgatggcggt ggcgatggcg gcgatcccct cgccgcgacc ggtgagcccg aggccgtcgg
     7561 tcgacgtccc cgacaacgac accgaggcgc cgcaggcggc cgacagggca acctcggcga
     7621 gctcgcggtg ccgaccgatc ttcgggcggt tgccgatcac ctgcacggcg acgttgccga
     7681 tctcgaagcc cgcctcgcgg acgaggcgcg cagcctcggc gagcagggcg cgcccggacg
     7741 cgccggcata ctccgggcgg tcggtgccga aatgggtgcc taggtcgcca atcccggccg
     7801 cggtgaagag cgcgtcgcag gcggcgtggc aggcgacgtc ggcatccgag tgcccgtcca
     7861 acccgcgctc ccctggccag tacagcccgg cgacccacag ctcgcgctcg gagccgtcgg
     7921 gcgcgaaggc atggacgtcg accccgaccc ccacccgcgg aatgaccggc acgaccggct
     7981 caaggctcat cgcccgagcg tcccacacca gggcgcgcgc cggagcacgg accacgcgcg
     8041 tcaggaggtt gcgctgtggt cccagtcgat cggcgtgcgg atcaccttgt gcaaagccgc
     8101 ctgagcgacc ggcttgacga tgagccggtc gacgttgacg tgctgcggca gccggacgca
     8161 aaacgcgacg cactcggcga catcggcggc cacgagcgga cggtcgacac cggcatacac
     8221 cgcgtcggcc ttgccctggt cgccgtggaa gcgggtgagc gagaagccgt cggtccacac
     8281 catgcccggg tcgatctcga tcacccggat cggctcaccg gcgagctcca gccgcagcgt
     8341 ctcgaccaca gccgtcgcgc cgtgcttggc cgccgtgtag cccccgccgc cctcatagac
     8401 cagcagtccg gcgatggacg agacgacgac gatggtgccg ccggtcggtg agcggcgcag
     8461 ggccggcaac agcgcctggg tgacctgcaa gacgccgatg acgttcgatt cgtacatcgc
     8521 gcgccactcg tcaggcttgc cgtcctcgac ccgctcgctg ccgatcgcgc cgcccgcgtt
     8581 gttgaccagg acgtcgcacg gcccgatctc cgcggcagcc gcagccacgc tcgccgcgtc
     8641 agtcacgtcc aggacgatcg cgcgggcggt caccgagggg tatgccgcgc ggatcgcggc
     8701 cgcggtctcc tccagcggcc cggggcggcg gccacagacg acgacatcga agccgtcctg
     8761 ggccaggcgg gttgcagtgg cagcaccgat cccagtgccc cctccggtga taacagcaac
     8821 gggtgccatg gcgggtcctt tcaccctcgg tggtcgacga ggcgctcggc gtgggcgagg
     8881 tctgcgggcg aggtgatctt gtatgccgcg gggtcacctg ggacggtgcc gacgcgcagg
     8941 ccaagcaact cgacgagccc ggcatcgtcg gtggccaggc tgccatgcgc cgcgtgggcg
     9001 gcgagcaaca catcgcggcg gaagccctgc ggagtttgga cgatccgcag ggcggcccgg
     9061 tccggggtcg acaccacgaa accctcggcg tcgacgacct tgacggtgtc gaccaccggc
     9121 atcaccggca cgacggccgg ggccgcctcg gtcaccgctg cggcgacccg ctcgaagacc
     9181 gcgctcggag tgaaggcccg cgccgcgtcg tgcacgagga ccacatcgca cgcctcgggc
     9241 agccaccgca ccccggccaa gacagagtcg ccgcgctcgg ccccaccggg aaccacctgc
     9301 acgggtatgc cgtgttcgcg gctcagcggc tcaacgaggt cgagcgcctc atcgacatgg
     9361 ctggctggtg cggcaaccac gacggcagca ggacgggccg acagagccgc gcggacggca
     9421 tactccagca gcgggcgacc acccacgagg acaaacgcct tggggacgga agcggcgagc
     9481 cgagagccgg agcccgcggc gacgatgacg acgccgacgg acatctcccg ctccgatcag
     9541 gcgcgccgga tcaggacgcg aggacctcgt cgaggatgga ctcggccttc tcctcgttgg
     9601 tcttctcggc gagcgcgagc tcggagacga ggatctgacg ggccttggcg agcatgcgct
     9661 tctcgccggc cgacaggcca cggtccttgt cgcggcgcca caggtcgcgg acgacctcgg
     9721 caaccttgat gacgtcgccc gaggcgagct tctcgaggtt ggccttgtaa cggcgcgacc
     9781 agttggtcgg ctcctcggtg taggcggcgc gcagcacctg gaacacccgg tcgagaccct
     9841 cttgacccac cacatcgcgg acgccgatgt cttcacagct gtccgcgggg acctggatgg
     9901 tcaggtcgcc ttgcgcgacc ttgagcgtga ggtagagccg ctccactccc ttgatcgtgc
     9961 gggtgttgat ttcctggatc agtgctgcac cgtggtgcgg gtacacgacc gtctcgccga
    10021 ccttgaagac catatggtgt taaccccttt cgcgactctc aggataccac gggcccaccg
    10081 acagccgcac gccgagaagc gcgtttgcgc aggtcagagg ctgtgccagg ggatccgggt
    10141 cgccgagacg ggccttgaca accgtcggtc accgtgctct cgcgcccctc cgcccgcccg
    10201 cctggggacg cggcataccc ctgaggtatg ccgccgctca cctggacgtg tccatcgcca
    10261 ccccctcccg gccggaccga ggccggggca taccgctacg ctgtgccccg tgacgcgact
    10321 ccaccgggcc ctcgccgtga ccggcgccgc ggcggccact gccgcccttt ccgcctgctc
    10381 gttcttctcg ccggtgcaga cggacaacaa atacattccg gccgacggtg tccccgtgca
    10441 actgggcgtc attgccggcc gcaacctggc catcgtcgcc gacgcagccg gcggccccgg
    10501 cgccctgacc ggtgccgtgc tcaaccaggg cgacgcagag gccgtggtgg gtttcctcac
    10561 ccgtgaggag tccgaggcgg gcgcctccgt caacacccgg ctgagcctgg gtgggcgcga
    10621 gtccaagccg atcagcggcg tcgtgttcga gaaggtgacc agtgcgcccg gcacgatgac
    10681 cgacatctac ctggtgacca ccgagggcaa gcaactcgtc tcggtgccgg tcatcgtgcc
    10741 gggcgcaccg gtcaccttct cgaacacgac gccgctgatc g
//
LOCUS       DAOMZY010000085        10778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5724_length_10778_cov_6.452952, whole genome shotgun sequence.
ACCESSION   DAOMZY010000085 DAOMZY010000000
VERSION     DAOMZY010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 10778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10778
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5724_length_10778_cov_6.452952"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="PLK64_06530"
     CDS             <1..671
                     /locus_tag="PLK64_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HPV79433.1"
                     /translation="LVNTAMAAVTLLQLGYAASEVERAMALEPVVPGRMERVPGGPDD
                     PRCVVDYAHTPEAVDAALAALRPSTAGRLVVVLGAGGDRDTGKRAAMGAAGARWADDL
                     IVTDDNPRSEDPAVIRDQVLAGARSVLPADPDGTTSARSLFSSDAGRAAHIERAVRLA
                     REQGRPVDNTVVVLGKGHETGQDIGGTVHPFDDRDALAAALRGAPYRPEGNDSGHVKT
                     EGEL"
     gene            685..2136
                     /gene="murF"
                     /locus_tag="PLK64_06535"
     CDS             685..2136
                     /gene="murF"
                     /locus_tag="PLK64_06535"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPV79434.1"
                     /translation="MIPLRLDHLARAAGAVLMDAHGRPAGPDAPEGRVLVDGPVVTDS
                     RECRPGSLYVARVGEQADGHAFIGAAQEAGAVAALTSRPVGGVAHLLTDDVQTAFGAI
                     ARAVVDAAPDLTIVGITGSSGKTSTKDLLAQVLRTGAETVAPEGSYNGEIGVPLTVCR
                     ITPTTRFLVAEMGSRGIGHIAYLTRIAPPRIGIVLNVGQAHVGEFGGRDNIARAKAEL
                     VAALPASGVAILNADDPAVAAMATATTARVVLVGESAAANIRAEEVELDESGRPSFRV
                     LSPAGTAQVRLRLHGRHHVGNALSVIAAALECGLPLDDIVAALAVAGPVSRWRMEVTE
                     RADGVTIVNDAYNANPDSMRAALRALRAMKGPADAGPERRTWAVLGEMFELGPDSHTE
                     HQQVGAVAVAQDVDRLVVVGAGAGLIGDGAVAAGMPPAHLARVPDTDAAYALLTENLR
                     RGDVVLFKSSRDAGLRWLGDRVAETVHGNEVRS"
     gene            2133..3215
                     /gene="mraY"
                     /locus_tag="PLK64_06540"
     CDS             2133..3215
                     /gene="mraY"
                     /locus_tag="PLK64_06540"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPV79435.1"
                     /translation="MKGVLVAAATSLVLALLGTPWFIKWLVKRQYGQFIRDDGPTSHR
                     TKRGTPTMGGTVIVGATLAAYFLAHLVLGTWPSVSGLLVLFLMTGLALVGFLDDYIKI
                     SRQRSLGLRTKQKLFGQGLVGVTFAVLALQFENAGYRTPASTAISFVRDTRFDLAFAG
                     PAIGLVLFVLWANFIITAMSNGANLTDGLDGLATGASTMIFGAYLLIGIWTSNQNCQT
                     NPGLKCYEVRDSLDLAIVSAAAMGACFGFLWWNASPAKIFMGDTGSLGLGGLIAGLAI
                     TTRTEFLSVILAGLFVLETLSVLIQVSVFKATRKRFFRMAPLHHHFEMLGWEQITVVI
                     RFWIIAGLFAALGLGIFYAEWVVGST"
     gene            3212..4774
                     /gene="murD"
                     /locus_tag="PLK64_06545"
     CDS             3212..4774
                     /gene="murD"
                     /locus_tag="PLK64_06545"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPV79436.1"
                     /translation="MSELQDFADPIDDGYEPRPGLTHRDADWSGLRVLVAGLGISGFA
                     AADALWERGAVVTVVDASAPGASRTVDERAQILAVLGVDVRLGPEHTAGMPSDRAIDL
                     VVTSPGWRPTHPVLADAAAHGIPIWGEVELAWRMRAREGAAPWLTVTGTNGKTTTVNM
                     LASILRAAGLRATSAGNVGTPLLEAVLHPQPYDVIAVELSSFQLFWQRSLSPVASACL
                     NVAADHIDWHGSFEDYLRAKGRVFRNTHLACIYNVADPLTEQLVIDADVIEGCRAVGF
                     TLGIPALSMVGLVDEILADRAFVEQRHHSAAELATIADVQGEAPSIAPHYLANALAAA
                     ALARAYGVGPLSVRDGLRAFRPDPHRIAEVGTVDGVRYVNDSKATNPHAAEAALASFD
                     SVVWVAGGLLKGADIEALVEKVAGKLRGVVLIGADRALIAQALARHAPDVPVVDAGGP
                     DTGGMQTTGEDVDRVMDRVVAHAATLARPGDVVLLAPAAASMDMFANYGERGSAFERA
                     VGRFGERPGQRS"
     gene            4771..6072
                     /gene="ftsW"
                     /locus_tag="PLK64_06550"
     CDS             4771..6072
                     /gene="ftsW"
                     /locus_tag="PLK64_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HPV79437.1"
                     /translation="MTSTVDRSARAPRAPRATRPGDTGAAHSWLPASLRPLAARFDSP
                     VTSYYVLVGVTTALLLIGLVMVLSASMITSYKDDGSSYAVFLNQARFAGIGLVGAFIA
                     SRLTVKWWKRLAVPSLAAAMVLQALVFVPGLGLSKGGNRNWLKLTSTLSLQPSELTKI
                     GLVLVGALILANKRASLGRVSHAVIPYLVPVAAITIGLVLAGHDLGTALVLIGIVGAV
                     MYAAGVPGRLFATAAVGAALLATALVVTSSNRMDRIGNWLSGSCTDPNGECGQSVHGL
                     YALADGGWWGVGLGASKEKWSWLPEAHNDFIFAIIGEELGLPGTFVILALFALLASAC
                     YRIVTRSTDQFVRIAAAGAMAWILFQAMINIGAVIGLLPVIGVPLPFVSAGGSALVTT
                     LVAMGMLVSFARAEPGAAEILAARPGVIQRSLAVLPRRRAR"
     gene            6094..7236
                     /gene="murG"
                     /locus_tag="PLK64_06555"
     CDS             6094..7236
                     /gene="murG"
                     /locus_tag="PLK64_06555"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HPV79438.1"
                     /translation="MTPFTRNRRVRPADSGGELVSVLLAGGGTAGHVSPLLATADALR
                     RRRPDLTVTALGTQTGLEARIVPARGYDLRFVPRVPLPRKPDANLVRFPGALRAGVEA
                     AGRAIDETGAQVVVGFGGYVSSPAYLAARRRGIPIVVHEQNARPGFANRLGARLTPYV
                     ATTFAATHLAHATCIGMPLRREISTLDRAALRADARAELGLDADRTTLLVTGGSLGAA
                     RLNETMAARSRDITDAGIQVFHAAGNGKGFVPDVSPSGAPYIVREYVDRMDLAYAAAD
                     LVLCRSGASTVCELTAVGLPAVYVPLPFGNGEQRVNAADVVAAGGGLVVEDAALTPVW
                     VDVVLLPLLTDADRIARMGAAAATVGRRDADEALVDLVLAAFAGDR"
     gene            7233..8735
                     /gene="murC"
                     /locus_tag="PLK64_06560"
     CDS             7233..8735
                     /gene="murC"
                     /locus_tag="PLK64_06560"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPV79439.1"
                     /translation="MTLSSGLPPTRFDYAAPVPPIAALGAVHLIGIGGAGMSAIARML
                     LARGLLVTGSDSRESASVDALTRDGIGIRIGQDAANVTGVQTVVVSSAIREDNPELAA
                     ARAAGLRVMHRSQALAALAADSPVRIAIAGANGKTTTSSMLAVVLTLAGRDPSYAVGG
                     DPVQLDANARAGTGEEFVIEADESDGSFVGYHPQVALVTSVQPDHLDFYGTAEAVEAG
                     YRAFVDTLDPGGLLVACLDDPGARRLADLARSQGRRVVGYGFSADAQVRLSDARLGGL
                     GAEATIEVDDAAYPMRLSVPGEHNLLNAAGVLAVALFAVVASEPHGAGRSPERVIGWL
                     DLLSRFSGVRRRFERVGEAVGVVVVDDYAHNPAKVRAAVATGRGAVEAGGRLVVVFQP
                     HLYSRTRDFAAEFGAALAPADVVVVTDVYAAREDPLPGVTGALVADAVRGVRAGVHVE
                     FVPDLGDVVPALTGLVAPGDLVLTVGAGDITSIGPALLLALGARSIREGQ"
     gene            8819..9523
                     /locus_tag="PLK64_06565"
     CDS             8819..9523
                     /locus_tag="PLK64_06565"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HPV79440.1"
                     /translation="MRFEQRAHAARRRPWVLVGTAVAILTLMGGLGWVVWASPVLVVD
                     EVVVTGLDGADRDAALTAAAVPIGEPLARVDSAAVAQRVGSLPIVKRAVVTRSWPRTL
                     VVSVTARVGMLVVRNSEGTLQVVDEDGVAFRDVDQAPAGLAVVNGSGQRPAPEGLQAV
                     IEVLRILPADQRATVSAITVSSASMVTFALGEVEVVWGGRGSEEKKGKVLQVLLKTTP
                     APKVIDVSAPDTPVTR"
     gene            9832..>10778
                     /gene="ftsZ"
                     /locus_tag="PLK64_06570"
     CDS             9832..>10778
                     /gene="ftsZ"
                     /locus_tag="PLK64_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPV79441.1"
                     /translation="MGAAPQNYLAVIKVVGIGGGGVNAINRMIDVGLKGVEFIAINTD
                     AQALLMSDADVKLDVGRELTRGLGAGADPNVGRKAAEDHAEEIEEVLRGADMVFVTAG
                     EGGGTGTGGAPVVARIARSLGALTIGVVTRPFTFEGRRRANQAESGIGGLREEVDTLI
                     VIPNDRLLSISDRNVSMMDAFRSADQVLLSGVQGITDLITTPGLINLDFADVKSVMQG
                     AGSALMGIGSARGEDRAVQAAELAISSPLLEASIDGAHGVLLSISGGSDLGLFEINEA
                     ARLVQEAAHPEANIIFGTVIDDALGDEVRVTVIAAGFDGG"
BASE COUNT         1436 a         3879 c         3742 g         1721 t
ORIGIN      
        1 acctcgtcaa caccgccatg gccgctgtca ccctgctgca actggggtat gccgcgtccg
       61 aggtcgagcg cgcgatggcg cttgagcccg tcgtcccggg ccggatggaa cgggttccgg
      121 gtgggcccga cgacccgcgg tgcgtcgtcg actatgccca cacccccgag gccgtcgacg
      181 cggccctggc cgcccttcgc cccagcacgg cgggccggct cgttgtggtg ctcggcgccg
      241 gcggtgaccg agacaccggc aagcgggcgg ccatgggggc tgccggagcg cggtgggccg
      301 acgacctcat cgtcaccgac gacaaccccc gctcggagga tcccgccgtc atccgcgacc
      361 aggtcctcgc gggggcccgc tcggtccttc ccgccgaccc tgatggcacc accagtgccc
      421 gaagcctgtt cagctccgac gccggccggg ccgcccacat cgagcgcgcc gtgaggctcg
      481 cccgcgagca gggtcgcccc gtcgacaaca ccgtggtcgt cctcggcaag ggccatgaga
      541 ccggacagga catcggcggc accgtgcatc cctttgatga ccgtgacgcg cttgccgccg
      601 ccctgcgcgg agcgccctac cgcccagagg gcaatgacag tgggcacgtg aagaccgagg
      661 gcgagctatg acggaaggca atcgatgatc ccgctgcgcc tggaccacct cgcgcgggcc
      721 gccggtgccg tcctgatgga cgcgcacggc cgccctgcgg gccccgacgc gcccgagggg
      781 cgggtgctcg ttgacggccc cgtcgtcacc gactctcgtg aatgtcggcc tggcagcctc
      841 tatgtcgccc gcgtcggcga gcaggccgac gggcacgcct tcatcggcgc cgcccaggaa
      901 gccggtgcgg tcgccgcgct gacctcgcgc ccggtcggcg gggtcgccca cctgctaacc
      961 gacgacgtac agacggcctt cggcgccatc gcacgggccg tcgtcgacgc tgcccccgac
     1021 ctcaccatcg tcgggatcac ggggtcgtcc gggaagacct cgaccaagga cctgctcgcc
     1081 caggtcctgc gcaccggcgc cgagaccgtg gcccccgaag gctcctacaa cggcgagatc
     1141 ggcgtcccgc tgacggtctg ccgaatcacc ccgaccaccc gcttcctcgt cgccgagatg
     1201 gggtctcggg ggatcggcca catcgcctac ctcacccgga tcgcgccacc gcgcatcggg
     1261 atcgtcctca acgtggggca ggcgcacgtc ggcgagttcg gtggccggga caacatcgcc
     1321 cgcgccaagg ccgagctcgt cgcggccctg ccggccagcg gtgtcgccat cctcaacgcc
     1381 gacgaccctg cggttgccgc catggccacg gccaccacag cccgggtcgt tctcgtcggt
     1441 gagagcgccg ccgccaacat ccgcgccgaa gaggtcgaac tcgacgagtc cggccgaccg
     1501 agctttcggg tgctcagccc ggccgggacg gctcaggtgc ggttgcgtct gcacgggcgt
     1561 caccacgtcg gcaacgccct gagcgtcatc gccgcagccc tcgaatgcgg cctgccgctc
     1621 gacgacatcg tcgccgccct cgcggtggcc ggtccggtga gccgctggcg catggaggtc
     1681 accgagcggg ccgacggcgt caccatcgtc aacgacgcct acaacgccaa ccccgactcg
     1741 atgagggccg cgctgcgtgc gctgcgggcc atgaagggcc cggccgacgc cggacccgag
     1801 cggcggacgt gggcggtgct gggggagatg ttcgagctcg gcccggactc ccacaccgaa
     1861 catcagcagg tcggtgcggt cgctgtggcc caggacgtcg accggctggt cgtcgtgggc
     1921 gcgggcgccg gcctcattgg tgatggtgcc gtggcagcgg gtatgccgcc cgcgcacctc
     1981 gcgcgcgtgc ccgacaccga cgcggcatac gccctgctga ccgagaatct ccgccgaggt
     2041 gacgtcgtgt tgttcaagtc cagtcgcgac gcgggactac ggtggctcgg tgaccgcgtc
     2101 gccgagacgg tccacgggaa cgaggtcagg tcgtgaaggg cgttctggtg gcggctgcca
     2161 cctcgctcgt gctcgctctg ctgggcacgc cgtggttcat caagtggttg gtgaagcggc
     2221 agtacggcca gttcatccgc gatgacgggc ccacttcgca ccgcaccaag cgcggcaccc
     2281 cgacgatggg cggcaccgtc attgtcggag ccaccctcgc cgcctacttc ctcgcccacc
     2341 tcgtgctggg cacctggccg tccgtcagcg ggctgctcgt tctgttcctc atgaccgggc
     2401 tcgcgctggt cggcttcctc gacgactaca tcaagatctc ccggcaacgc agcctggggc
     2461 tgcgcaccaa gcagaagctg ttcggccagg gtctggtcgg ggtgaccttc gcggtgctcg
     2521 ccctgcagtt cgagaacgcc ggctatcgca ccccagcgtc gacggcgatt tcgttcgtcc
     2581 gcgatacgcg cttcgacctc gcgttcgctg gtccggcgat cgggctggtg ctcttcgtcc
     2641 tctgggccaa cttcatcatc accgcgatgt ccaacggcgc gaacctcacc gacggcctgg
     2701 acgggctggc gaccggtgcc tcgacgatga tcttcggcgc ctacctgctc atcgggatct
     2761 ggacgagcaa ccagaactgt cagaccaacc ccggcctcaa gtgctacgag gtgcgcgaca
     2821 gcctcgacct ggcgattgtc tcggcagcgg cgatgggcgc gtgtttcggg ttcctctggt
     2881 ggaacgcctc gcccgccaag atcttcatgg gtgacaccgg gtcgctcggc ctcggcgggc
     2941 tcatcgccgg cctggccatc acgacccgca ccgagttcct ctcggtcatc cttgccggac
     3001 tgttcgtcct ggagacgctg tcggtgctca tccaggtgag cgtcttcaaa gcgacccgaa
     3061 aacgcttctt ccggatggcg cccctccacc accacttcga gatgctcggg tgggagcaga
     3121 tcacggtggt catccgcttc tggatcatcg ccgggttgtt cgccgcactc ggcctgggca
     3181 tcttctacgc cgaatgggtg gtgggctcga catgagcgag ctgcaggact tcgctgatcc
     3241 gatcgacgac ggttatgagc cgcgcccggg cctcacccac cgcgacgccg actggtcggg
     3301 tctgcgggtg ctcgtcgcag gcctggggat cagcggcttc gccgcggccg atgcgctgtg
     3361 ggagcgcggg gccgtcgtca ccgtcgtcga tgccagtgcg ccgggagcat cccgaacggt
     3421 cgacgagcgc gcccagatcc tcgccgtcct cggcgtcgac gtccggctcg ggccggaaca
     3481 caccgccggt atgccgtctg atcgggccat cgatctcgtc gtcacctcgc cgggctggcg
     3541 ccccacccac ccggtgttgg ccgatgcggc cgcgcacggc atacccatct ggggggaggt
     3601 cgagctcgcg tggcggatgc gggcccgcga aggggcggcg ccctggctga ccgtgaccgg
     3661 caccaacggc aagacgacca cggtcaacat gctggccagc atcctgcggg ccgcgggcct
     3721 gcgggccacc agcgccggca acgtcggcac ccccttgctt gaggctgtgc tgcacccgca
     3781 gccctatgac gtcatcgccg tcgagttgtc gagcttccaa ctgttctggc agcgctcgct
     3841 gtctccggtg gcgtcggctt gtctcaacgt cgcggccgac cacatcgact ggcacgggtc
     3901 gttcgaggac tacctgcggg ccaagggccg ggtctttcgc aacacccacc tcgcctgcat
     3961 ctacaacgtc gccgacccct tgaccgagca gttggtcatc gacgccgacg tcatcgaagg
     4021 gtgccgagcg gtcgggttca cgttgggcat tccggcgctg tcgatggtcg ggttggtcga
     4081 cgagatcctg gccgaccggg ccttcgtcga gcagcgccac cactcggcgg ccgagttggc
     4141 cacgatcgcc gacgtccagg gcgaggcccc ctcgatcgcc ccgcactatc tggccaatgc
     4201 cctggcggcg gcggccctcg cgcgcgccta cggtgtcggt ccgctgtcgg tgcgtgacgg
     4261 tctgcgggcg ttccgccccg acccccaccg gatcgccgag gtcggcaccg tcgacggggt
     4321 ccgctacgtc aacgactcca aggcgaccaa cccgcacgcc gccgaggctg cgttggcgtc
     4381 gttcgacagc gtcgtctggg tcgccggcgg gctgctcaag ggggccgaca tcgaggccct
     4441 cgtcgagaag gtcgccggca aactgcgcgg ggtcgtcctc atcggcgccg atcgggcact
     4501 catcgcccag gctctggccc gacacgcgcc ggatgtcccc gtcgtcgacg ccggcgggcc
     4561 ggacactggg gggatgcaga ccacggggga agacgtcgac cgagtgatgg accgggtcgt
     4621 cgcgcacgcg gcgacgctgg cccgccccgg tgatgtcgtg ctgctcgcgc cggcggcggc
     4681 gtcgatggac atgttcgcca actacgggga gcgcggcagc gccttcgaac gcgccgtggg
     4741 gcggttcggc gagcggccgg gtcaacgctc gtgaccagca ccgtcgatcg ttccgcgcgc
     4801 gcgccccggg cgccccgggc gacccggccg ggcgacaccg gtgccgcaca ctcctggttg
     4861 ccggccagcc tgcggcccct cgccgcccgt ttcgacagcc cggtgacctc ctactacgtc
     4921 ctcgtcgggg tcaccacagc gctgctgctc atcgggttgg tcatggtcct gtcggcgtcg
     4981 atgatcacct cctacaagga cgacggctcg tcgtatgccg tgttcctcaa ccaggcgcgc
     5041 ttcgccggca tcgggttggt cggagcgttc atcgcctcgc ggctcaccgt caagtggtgg
     5101 aagcgtctgg ccgtgccctc cctcgccgcc gccatggtgc tgcaggcgtt ggtcttcgtc
     5161 cccggtcttg ggctctctaa gggtggcaac cgcaactggc tcaagctcac ctcgacgctg
     5221 tcgttgcagc cttccgagct caccaagatc ggcctggtcc tcgtgggggc gctcatcctc
     5281 gccaacaagc gggcctcgtt gggccgggtc tcgcacgccg tcattcccta tctcgtgccg
     5341 gtcgccgcca tcacgatcgg cctggtcctc gccggtcacg acctcgggac cgcgctggtt
     5401 ctcatcggga tcgtcggagc ggtgatgtat gccgcgggcg tgccgggccg gttgttcgcg
     5461 acggcggcgg tgggggcggc gctgctggcg acggctctcg tcgtcacgag cagcaaccgg
     5521 atggaccgga tcggcaactg gctgtccggg tcgtgcaccg accccaacgg cgagtgcggt
     5581 cagtccgtgc atggcctcta tgcgctcgcc gacggtggct ggtggggagt ggggctgggc
     5641 gccagcaagg agaagtggtc gtggcttccg gaggcccaca acgacttcat cttcgccatc
     5701 atcggcgagg agttggggct gccaggcacc ttcgtcatcc tcgccctctt cgccttgctt
     5761 gcatcggcct gctaccgcat cgtcacccgc tcgaccgatc agttcgtccg gatcgccgcg
     5821 gccggggcga tggcctggat cctcttccag gcgatgatca acatcggcgc ggtcatcggg
     5881 ttgctcccgg tcatcggggt gccgctgcca ttcgtttcag cgggcggctc ggccttggtg
     5941 acgaccctcg tcgcgatggg catgctcgtg tcgttcgccc gggccgagcc cggcgccgcc
     6001 gagatcctcg ctgcgcgtcc tggggtgatc cagcggtccc tggccgtcct gccccgccgc
     6061 cgcgcgcgct gaccccaacc cggagttgcc acgatgaccc ccttcactcg caatcgccgt
     6121 gtccgccccg ccgactccgg cggcgagttg gtctcggtcc tgctcgccgg cggcggcacc
     6181 gcggggcatg tgtcaccgct gctggccacg gccgacgcgt tgcgccggcg gcgccccgac
     6241 ctcaccgtga cggcgctggg cacccagacc gggcttgagg cgcggatcgt ccccgcgagg
     6301 ggctacgacc tgcggttcgt cccgcgggtg ccgctgcccc gcaaacccga cgccaacctc
     6361 gtgcgcttcc ccggggccct gcgcgcaggg gtcgaggccg ccggacgggc catcgacgag
     6421 accggcgccc aggtggtcgt cggcttcggc gggtacgtct cctcgcccgc ttacctcgcg
     6481 gcccggcgcc gcggcatacc catcgtcgtt cacgagcaga acgcccgccc tgggttcgcc
     6541 aaccggttgg gggcgcggct gactccctac gtcgccacga ccttcgccgc cacccacctg
     6601 gcgcacgcaa cctgcatcgg tatgccgctg cgccgcgaga tctccaccct cgaccgggcc
     6661 gccctgcgcg ccgacgcacg ggccgagctg gggctcgatg ccgaccgcac cacgctgctg
     6721 gtgaccggcg ggtcgctcgg cgccgcccgc ctcaacgaga cgatggccgc ccgcagccgc
     6781 gatatcaccg acgcggggat ccaggtcttc cacgcggcgg gcaacggcaa agggttcgtg
     6841 ccggacgtca gtccgtcggg cgcgccctac atcgtgcggg agtacgtcga ccggatggac
     6901 ctcgcatatg ccgccgccga cctcgtcctg tgccggtcgg gagcgagcac ggtgtgcgag
     6961 ctcaccgcgg tggggctgcc cgccgtctat gtcccgctgc ccttcggcaa cggtgagcag
     7021 cgggtcaacg ccgccgacgt cgtcgccgcc gggggagggc tggtcgtcga ggacgcggcg
     7081 ctgacccccg tctgggtcga tgtcgtcctg ttgccgctgc tcaccgacgc cgaccgcatc
     7141 gcgcggatgg gcgcggccgc ggcgaccgtc ggtcgccgcg atgccgacga agcgctcgtc
     7201 gatctcgtgc tggccgcctt cgcgggggat cgatgacgct gtcgtccggg ttgccgccga
     7261 cccgcttcga ctatgccgcc ccggtgccgc ccatcgcggc gctcggggct gttcatctga
     7321 tcggcatcgg cggcgccggc atgtcggcga tcgcgcggat gctgctggcg cgcggtctcc
     7381 tcgtcaccgg gtccgactcg cgtgaatcgg cgtcggtgga tgcactgacc cgcgacggga
     7441 tcggcatccg catcggccag gacgcggcga acgtcaccgg cgttcagacc gtcgtggtgt
     7501 cttccgcgat ccgtgaggac aacccggagt tggctgcggc tcgcgccgcc gggctgcggg
     7561 tcatgcatcg gtcgcaggcg ctcgcggcgc tcgctgccga ctcgccggtg cggatcgcca
     7621 tcgcgggggc caacggcaag acgacgacct cgtcgatgct cgccgtcgtc ctcacgctgg
     7681 ccggacgcga cccgtcgtat gccgtcgggg gagaccccgt gcagttggac gccaacgccc
     7741 gggccggcac cggtgaggag ttcgtcatcg aggccgacga gagcgacggc tccttcgtcg
     7801 gctatcaccc gcaggtcgct ctggtgacca gcgtgcagcc cgaccacctc gacttctacg
     7861 gcaccgccga ggcggtggag gcgggctatc gggccttcgt cgacaccctt gacccaggcg
     7921 gcctgctcgt cgcctgcctc gacgacccgg gagcccgacg gttggccgac ctggcccgca
     7981 gccagggccg ccgcgtcgtt ggctacggct tcagcgccga tgcgcaggtc cggctatccg
     8041 atgcccggtt ggggggcctc ggggccgagg cgaccatcga agtcgacgac gcggcatacc
     8101 cgatgcggtt gtccgtgccc ggcgagcaca acctcctcaa cgccgccggg gtgttggccg
     8161 tggcgctctt cgccgtcgtc gcgagcgaac cgcacggggc tgggcgcagc ccggagcgag
     8221 tgatcggttg gctggacctc ttgtcgaggt ttagtggggt tcggcgccgt tttgagcgcg
     8281 tgggggaggc tgtcggcgtt gtggtcgtgg acgactacgc ccacaacccg gccaaggtcc
     8341 gggcggccgt cgccaccggg cgcggtgcgg tcgaggccgg ggggcggctc gtcgtcgtct
     8401 tccagccgca cctctactcc cgcacgcgag acttcgccgc cgagttcgga gcggcgctcg
     8461 caccggccga cgtcgtcgtc gtcaccgatg tgtatgccgc ccgagaggac cccctgccgg
     8521 gcgtgaccgg cgcgctggtc gccgacgccg tgaggggggt tcgtgcaggc gtccacgtgg
     8581 agttcgtccc cgatctgggc gatgtcgtcc cagcactgac cgggctggtc gctcccggcg
     8641 acctcgtcct cacggtgggg gccggggaca tcacctcgat cgggccagcc ctcctcctgg
     8701 cgctgggcgc ccggtcgatc agggagggcc agtgagggac accctgaggt cgtgggtgag
     8761 ggccgtccag cgacgggtcc gaggcgacgg tggtccccgg cggttgccgt cggcacgagt
     8821 gcgtttcgaa cagcgggccc acgccgcgcg ccgccgaccc tgggtgctcg tcggaaccgc
     8881 ggtggcgatc ctgacgctca tgggcggcct gggctgggtg gtctgggcca gtccggtgct
     8941 cgtcgtcgac gaggtcgtcg tcacgggcct tgacggtgcc gaccgtgatg ccgcgctgac
     9001 tgcggctgcg gtcccgatcg gtgaaccctt ggcccgggtc gattccgcgg cagtcgccca
     9061 gcgggtcggc agcctcccga tcgtcaagcg ggcggtcgtc acccgcagct ggccgcgcac
     9121 cctcgtggtc tcggtgaccg cgcgtgtcgg gatgctcgtc gtgcgcaact ccgagggcac
     9181 cttgcaggtc gttgacgagg acggggtggc cttccgcgat gtcgaccaag caccggccgg
     9241 gttggccgtc gtcaacggca gcgggcagcg gccggcaccg gaaggcctgc aggcggtgat
     9301 cgaggtgctg cggatcctgc cggccgacca gcgcgccacg gtctcggcga tcacggtatc
     9361 gagcgcgagc atggtgacct tcgccctcgg cgaggtggag gtcgtgtggg gcggccgtgg
     9421 gtcggaggag aagaagggga aggtccttca ggtgctcctc aagaccaccc ctgcgccgaa
     9481 ggtcatcgac gtcagcgctc ctgacacccc ggtgacccgc taactggcac aacagtcaca
     9541 tcagtcacac aagcctcaga agtcccacgc gtcgcgggga tatacccgaa tcttgacgtt
     9601 aggtccaaga cacgccgagt gggtcgttgg aaaccctcgg tctagtgcct acggtcggtg
     9661 cgacggtaga gaccgagaaa cactgtatct cgactagacc ttcaggttcg gcagcggttc
     9721 tccggcgtgt cggacggggc ggttctccgc ctcgaccgca ggccgtagtg ccggggtcgg
     9781 gcccgatcgg acgacacgaa tcgcgccaga acgcaacgaa aggccaccac cgtgggtgca
     9841 gcaccgcaga actacctcgc cgtcatcaaa gtcgtcggca tcggtggtgg cggcgtcaac
     9901 gccatcaacc ggatgatcga cgtcggcctc aagggcgtgg agttcatcgc catcaacacc
     9961 gacgcccagg cgttgctgat gagcgatgcc gacgtgaagc tcgacgtcgg ccgcgagctg
    10021 acccgcgggc tcggcgctgg cgccgacccc aacgtcgggc gcaaggcggc cgaggaccac
    10081 gccgaggaga tcgaagaggt cctccgcgga gccgacatgg tgttcgtcac cgccggcgag
    10141 ggtggcggca cgggcaccgg tggcgcgccg gtggtcgccc gcatcgcccg gtccttgggc
    10201 gccctcacca tcggtgtcgt cacgcggccg ttcaccttcg aggggcgtcg tcgggccaac
    10261 caggccgagt cggggatcgg cgggctgcgc gaagaggtcg acaccctcat cgtcatcccc
    10321 aacgaccggc tgctgtcgat cagcgaccgc aacgtctcga tgatggacgc cttccgcagc
    10381 gccgaccagg tgctcctctc cggcgtccag ggcatcaccg acctcatcac cacgccgggt
    10441 ctgatcaacc tcgacttcgc cgacgtcaag tcggtcatgc agggcgccgg ctcggcgctc
    10501 atgggcatcg ggtcggcgcg tggcgaggac cgagccgtcc aggctgccga gctcgccatc
    10561 tcctccccgc tgctggaggc gagcatcgac ggcgcccacg gtgtgctctt gtcgatctct
    10621 ggtgggtctg accttgggct gttcgagatc aatgaggccg ctcgcctggt ccaggaagcc
    10681 gcccaccccg aggccaacat catcttcgga accgtcatcg acgacgccct gggcgacgag
    10741 gtgcgcgtca ccgtcatcgc cgccggcttc gacggcgg
//
LOCUS       DAOMZY010000086        10753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5733_length_10753_cov_6.043279, whole genome shotgun sequence.
ACCESSION   DAOMZY010000086 DAOMZY010000000
VERSION     DAOMZY010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 10753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10753
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5733_length_10753_cov_6.043279"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1773)
                     /locus_tag="PLK64_06575"
     CDS             complement(<1..1773)
                     /locus_tag="PLK64_06575"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HPV79442.1"
                     /translation="MCVLAGAGTGKTRAITHRIAYGVHSGAYVPQRVLAVTFTARAAG
                     EMRTRLRELGVGGVPARTFHAAALRQLHFFWPQAVGGAAPEVLASKAPVVAESVSRLR
                     MQFDRTAIRDLAAEIEWGKVSLLTPQSYAAGARRAGREAPGIDHTAMARVWESYEERK
                     TERGVIDFEDVLLLMAGILDERMDIARTVRDQYRHFVVDEYQDVNALQQRLLDLWLGG
                     RDELCVVGDPAQTIYTFTGATPEHLLTFRSRYPQARLVRLVRNYRSTPQILGLANLVV
                     RSARPSLRRPGVGGSAAARALPDHVDLQAQGEPGPQPRLTAYPDDPAEAAGVAGAIKA
                     LVDGGRPPGEIAVLFRTNAQSEGLESALAALDVPYLVRGGERFFARQEVRGAIVLLRG
                     AVRGDDGSRPLPELVRDVLTGVGWAATPPTSGGAQRERWESLMALAGLADDLVAASPS
                     ARMGDLVRELDERAAAQHAPAVAGVTLASLHAAKGLEWDTVFIVGCSDGFLPIVMAEG
                     EVAIEEERRLFYVGLTRARRDLQLSWSGARTPGARATRRPSRFLDPAASILGEGARSA
                     PRRSGVAGGSGSSPSAPRQPRLPSV"
     gene            complement(2176..2934)
                     /locus_tag="PLK64_06580"
     CDS             complement(2176..2934)
                     /locus_tag="PLK64_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPV79443.1"
                     /translation="MGSHTGAVAIVTGASRGIGFGIAQRLVTEGARVVITARKPEPLA
                     EAVAALGGSEYAVGLAGNAADEAHQDEAIALARAAFGGLDYLVNNTGINPVYGPILDV
                     DVDVARKVLDVNVVAAFSWIKKAVATGLGSPDHPGSVVNIASIAGLRASGTIGWYGVS
                     KAALIHLTMELGHQLGPDVRVNAVAPAVVKTQFAQALYVGREEKVAAAYPLKRLGVPE
                     DIAGAVSFLLSRDAAWMTGQTLVVDGGVTLGGAL"
     gene            complement(2969..4123)
                     /locus_tag="PLK64_06585"
     CDS             complement(2969..4123)
                     /locus_tag="PLK64_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPV79444.1"
                     /translation="MPRNLYSEEHEDFRASVREFVERSLKPRVEEMISLKSIPRDIWL
                     EAGKQGFFGMDIPEEFGGAGAGDYRFNAVLAEEWAHFNAATAACFGIHADVCPPYIVD
                     LGTEEQKQRWLPGIASGEKIIAIGMTEPSGGSDLAALKTTAVRADDGSGDWIINGSKT
                     FITNGYQCDLCIVAARTDPTKGPKGITLFMVERGMDGFVNGRKLDKVGQDESDTAELF
                     FENVRVSDANRLGPENGGFIAMMQRLPQERVGAAVSNVAHAFQIFRETLVYTKERKAF
                     GQSIGAFQHNKFKLAEMLTKLEVTQAFVDDCVDAHARHELSSVDASKAKWWSAQVQND
                     VLDECVQLHGGYGFMNEYRAGRAWRDARVTMIWAGSNEIMKELIGRDLGL"
     gene            4206..4844
                     /locus_tag="PLK64_06590"
     CDS             4206..4844
                     /locus_tag="PLK64_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPV79445.1"
                     /translation="MSTTPAAVTPASETVQRLFSAAAEAFAAKGFHATTTRDIASRAG
                     LSPAGVYVHFASKEDLLFHLSRDGHEEALALVTEAVAGADSPAEALAATMSRFSAWHA
                     ENYAIARVVQHEFPHLSDAHRAEVLALRKRIDQAVRDVLAAGKQSGDFLLDDIPDTAL
                     ALLSIIVDVARWYTPALHRTSAQIGATNAALGLRLVGYRGGQINQSGSRAIH"
     gene            complement(4813..5757)
                     /locus_tag="PLK64_06595"
     CDS             complement(4813..5757)
                     /locus_tag="PLK64_06595"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HPV79446.1"
                     /translation="MLSVLAITIPIYACVAVGHVAVRRGLLSRSDMRVLTTFVISISL
                     PCMLFLAIGTRPVRDILNPTYLAAYALGSVLAYAVGYGYGRLRGAGPAGRAFDGLGMS
                     GSNSGFVGYPLLLLTLPDVAGLVLGLSMIVENLLVLPLMFLLAEGRAQGHDSRAKVLA
                     TYATRLTRNPLLIGLVAGLIVSLSGVHLPEAVLRTADLFSRAGTAVALFAIGGLLATF
                     PRGAELRRVLALSLGKLLLHPLLVAGALLVLVGLGLPALDPALRIGLLVTGALPIMST
                     MPVLAHPYGESEPIAGVLLVTTGVSFLTLSGLLGILTG"
     gene            complement(5776..6690)
                     /locus_tag="PLK64_06600"
     CDS             complement(5776..6690)
                     /locus_tag="PLK64_06600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HPV79447.1"
                     /translation="MRLGTQISYFTWPDAPASIGPTFGRIAQDADAAGMDSLWVMDHF
                     FQISMIGPAELDMLEGYTALAFAAGQTQRIQLGTMVTGVTYRYPGVLAKTVTTLDVLS
                     GGRAWLGIGAAWNEEEHLGLGVPYPALAERFERLEETLQIALQMWRGDESPYAGQHYQ
                     LTRPLNVPQSLTRPHPPILIGGGGEKKTLRLVAQYADACNIFDMGPAAITAKYDVLRG
                     HCDALGRNYADIEKTVLSRLTLGDGSPDATGTPTVSVAEGVERMGRLAEIGTDHVILG
                     FANVATPGAFDQVAELVRQVAPIVPAGR"
     gene            6802..7596
                     /locus_tag="PLK64_06605"
     CDS             6802..7596
                     /locus_tag="PLK64_06605"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPV79448.1"
                     /translation="MSTTATPPPAAQPPARRRTGRFGEKEWEVYDAWTPVTLRGDVTV
                     VLVHGGLWQAAYDRAHLRPLGAALARDGWPVASVEYTRVGMPRGGWPGTAESVLAAID
                     AVAADPELPDRLVAVGHSAGGHLAVWAASNGRCPALTGVVSLAGVLDLRLADTDDLGS
                     GAVRALLGGEPTECPQAWVEADPMAARLHVPAVLLHGRSDDLVPSEVSASYLRSRGPL
                     DAPCRLELVPACDHFGIIDPRHAAYERVLAAIGEVAGTSPAGEPRS"
     gene            7593..8171
                     /locus_tag="PLK64_06610"
     CDS             7593..8171
                     /locus_tag="PLK64_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HPV79449.1"
                     /translation="MTGPVSPTGPPTTAGHSVLVVPIPALETFVAARHAHYDRDYVSA
                     DPTFAHAHVTILGPWLTQADLADPVRANAALTAVRDIASATRGFDIDFAHVDTFPNGI
                     IHLVPEPAEPFAALTAAVYREFPAYPPYAGQFGVVQPHLTLDAVGAGVTEAVVRGWVA
                     DLLPLRVRADRLHVSWYEAGRCQTLAVFPFGR"
     gene            complement(8202..9884)
                     /gene="ettA"
                     /locus_tag="PLK64_06615"
     CDS             complement(8202..9884)
                     /gene="ettA"
                     /locus_tag="PLK64_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003786651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HPV79450.1"
                     /translation="MAEFIYTMTKARKAVGDKLILDDVTMSFFPTAKIGVVGPNGAGK
                     STILRIMAGLDQPSNGEARLSPGYTVGILLQEPPLNESKTVLGNVEEGAGEIKAKLDR
                     YNAIALEMGEPDADYEVLGDEMAKLQDEIDHANAWDLDSQLEQAMDALRCPPGDADVT
                     VLSGGERRRVALCKLLLQKPDLLLLDEPTNHLDAESVQWLEQHLAQYPGAVLAVTHDR
                     YFLDNVAQWIAEVDRGRLYPYEGNYSTYLEKKAERLQIQGRKDVKLAKRLRDELEWVR
                     SNAKGRQTKSKARLARYEEMAAEADRTRKLDFEELAIPPGPRLGSQVIEVRKLRKGFG
                     DRVLIDNLSFTLPRNGIVGVIGPNGVGKTTLFKTIVGLEAPDSGKVKVGETVQISYVD
                     QGRSGLDPVKTLWEVVSDGLDFIKVGQVEIPSRAYVSQFGFKGPDQQKPAGVLSGGER
                     NRLNLALTLKQGGNLLLLDEPSNDLDVETLGSLENALLEFPGCAVVISHDRWFLDRVA
                     THILAYEGDEEDPAKWYWFEGNFEAYEANKVERLGIEATRPHRVTYRKLTRD"
     gene            complement(9993..10601)
                     /gene="ssb"
                     /locus_tag="PLK64_06620"
     CDS             complement(9993..10601)
                     /gene="ssb"
                     /locus_tag="PLK64_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPV79451.1"
                     /translation="MYESYVTVVGNLTADPVLRTTKAGKPFATFRMASTPRRWDGRER
                     AYVDGGTNFVSVVTFNSLAANVVASMAKGQPVIVYGRLRVNMWSTAEGHSNTSVEIDA
                     YHVGHDLMWGQSLFTKSPRAQFDTTDRLSEPEIQQALAEDVDGPSDIEDEDREPDWSP
                     INIDFTADAADPVVIEGAPLTGASSWSGANPETDAYVVAHAR"
BASE COUNT         1667 a         3733 c         3690 g         1663 t
ORIGIN      
        1 taccgacggc agtcgaggtt ggcgcggcgc gctgggcgac gacccggacc cgccggctac
       61 ccccgagcgc cgcggggccg agcgcgcccc ctccccgagg atcgacgcgg ccgggtcgag
      121 gaaccgtgac gggcgccggg tggcccgcgc ccccggggtc cgcgccccgg accaactcaa
      181 ctgcaggtcg cgccgcgcgc gggtcagccc gacgtagaag agccggcgtt cctcctcgat
      241 ggccacctcg ccctcggcca tgacgatggg caggaagccg tcggagcagc cgacgatgaa
      301 gacggtgtcc cactccaggc ccttggccgc gtgcagcgac gcgagggtca ccccggcgac
      361 ggcgggagcg tgctgcgccg cggcccgctc gtcaagctcg cggacgaggt cgcccatccg
      421 cgcgctgggg ctggctgcca cgaggtcgtc ggcgagcccg gccagggcca tgagggactc
      481 ccaacgctcg cgctgcgctc cccccgacgt cggcggggtc gcggcccacc ccacgccggt
      541 gagcacgtcg cgaaccagct cagggagcgg tctggacccg tcgtcaccgc gcaccgcgcc
      601 acgcagcagc acgatggccc cgcgcacctc ctgtcgggcg aagaaccgct cgccgccgcg
      661 gacgagatac ggcacatcca gcgcggcgag tgcggactcc aacccctcgg actgcgcatt
      721 ggtccggaac agcaccgcga tctcgcctgg cggccgcccc ccgtcgacca gagccttgat
      781 tgcgcccgcc acccccgccg cctcggccgg atcgtccggg tatgccgtga gcctgggttg
      841 cgggcccggc tcaccttggg cctggaggtc gacgtgatcg ggcagcgctc gggccgccgc
      901 ggaccctcca acacccggac ggcgcagcga cggccgggcc gagcggacga cgaggttggc
      961 caggccgagg atctgggggg tgctgcggta gttgcgcacc agccgcacga gccgagcctg
     1021 cgggtagcgg ctgcggaagg tcagcaggtg ctccggggtg gcccccgtga aggtgtagat
     1081 cgtctgggcc gggtcaccca cgacgcacaa ctcgtcgcga ccgcccagcc acaggtccag
     1141 cagccgctgc tgcagggcgt tgacgtcctg atattcgtcg acgacgaagt gtcgatattg
     1201 atcgcggacg gtgcgcgcga tgtccatccg ctcgtcgagg atgccggcca tgagcagcag
     1261 gacgtcctcg aagtcgatga ccccgcgctc ggtcttgcgc tcctcgtagc tttcccacac
     1321 ccgggccatc gccgtgtggt cgatgcccgg cgcctcgcgg cccgcccgcc gggcgcccgc
     1381 ggcatacgac tgcggggtca gcagcgagac cttgccccac tcgatctcgg ccgcgaggtc
     1441 gcggatggcg gtgcggtcga actgcatccg caaccgcgac acggactcag cgacaacggg
     1501 cgccttgctg gcaagcacct ccggggccgc gccgccgacc gcctgcggcc agaagaagtg
     1561 cagttggcgc aacgccgcgg cgtggaaggt gcgcgccggc actcccccga cgccgagctc
     1621 gcgaagtcgg gtgcgcatct cccccgccgc ccgcgcggtg aacgtcacgg cgagaacccg
     1681 ttgcggcaca taggcccccg agtggacgcc ataagcgatc cggtgggtga tggcccgggt
     1741 cttgccggtg cccgcgccgg ccagcacaca catcgggccg actgggctgg cggccacctc
     1801 acgctgttcg gggtcgaggg cggccaggat gtcgtcggct cgggagctcg cctcacccac
     1861 ggcggttcct ccctcgctgc gacggtatgc cggtcacccg gctcccgtcg atcctaggtc
     1921 tgcgcaccga cggccccgat gtatcagggc cgcgcgacgg tgctcttgca cggtgtaggg
     1981 cttcgggaag gtgccaggcc atcgatgcgc gccgcatcgg ccgggtgcgc gcccggacga
     2041 gacggcgccg cctggggcgc gaagcggtcc cgagtccagg ggtctcggga ccgcttcgtg
     2101 tgcccaatcg gccccggtgc gtgtcagggg gtcaggtgcg ccatcagacc gcggccaagt
     2161 gccggaggcc aatggctaga gagcgccgcc gagggtgacg ccgccgtcga ccacgagggt
     2221 ctggccggtc atccacgccg catcgcggga cagcaggaag ctcaccgctc ccgcgatgtc
     2281 ctccgggacg ccgagccgct tcagggggta tgccgcggcc accttctcct cgcggccgac
     2341 atagagcgcc tgagcgaact gggtcttgac gactgcgggc gcgaccgcgt tgacccgaac
     2401 gtccggaccg agttgatggc ccaactccat cgtcaggtgg atgagtgcag ccttggagac
     2461 gccgtaccac ccgatggtcc cgctggcccg aagcccggcg atcgaggcga tgttgacgac
     2521 tgaccctggg tggtcgggcg agcccaaccc ggttgcaacg gccttcttga tccacgagaa
     2581 ggcggcaacg acgttgacgt cgagcacctt gcgggccacg tcgacgtcga catcgaggat
     2641 cgggccgtag accgggttga tgccggtgtt gttgacgagg tagtccagcc ccccgaacgc
     2701 agcgcgagcc aacgcgatcg cctcgtcctg gtgagcctcg tccgcggcgt tcccggccag
     2761 cccgacggca tactcggaac cgcccaaggc agccaccgcc tcggcgaggg gttcgggttt
     2821 gcgagccgtg atgacgacgc gggcgccctc ggtgaccaac cgttgcgcga tcccgaaccc
     2881 gatgccccgg ctggcgccgg tgacgatggc gactgctccc gtgtgagaac ccatgacagg
     2941 aacgttcgac ggtgggtcgg acgccgactc agaggccgag gtcacggccg atgagttcct
     3001 tcatgatctc gttggagccg gcccagatca tggtgactcg ggcatcgcgc cacgcccggc
     3061 cggcccggta ctcgttcatg aagccgtagc cgccgtgcaa ctggacgcac tcgtcgagga
     3121 cgtcgttctg cacctgggcg gaccaccact tggccttgga cgcatcgacc gacgacaact
     3181 cgtgccgggc gtgggcgtcg acgcagtcgt cgacaaaggc ctgggtgact tcgagcttgg
     3241 tcagcatctc ggcgagcttg aacttgttgt gctggaacgc cccgatggac tggccgaacg
     3301 ccttgcgctc cttggtgtag acgagggtct cgcggaagat ctggaacgcg tgagccacgt
     3361 tggagaccgc ggcgccgacc cgctcctgag gcaggcgctg catcatcgcg atgaagccgc
     3421 cgttttcggg cccgagccgg ttggcgtcgg acacccggac gttctcgaag aacaactcag
     3481 ccgtgtcgga ctcgtcctgg ccgaccttgt cgagcttgcg gccgttgacg aagccgtcca
     3541 tgccgcgctc gaccatgaac aacgtgatgc ccttcgggcc cttggtcggg tcggtgcggg
     3601 ccgcgacgat gcacaggtca cactggtagc cgttggtgat gaaggtcttg gagccgttga
     3661 tgatccagtc gccggagccg tcgtcggccc tcaccgccgt cgtcttaagt gcggcgagat
     3721 ccgacccgcc ggacggctca gtcatgccga tggcgatgat cttctcgccg gacgcgatgc
     3781 cggggagcca gcgctgcttc tgctcctcgg tgcccaggtc gacgatgtag ggcgggcaga
     3841 catcggcgtg gatgccgaag cacgcggccg tggccgcgtt gaagtgggcc cactcctcgg
     3901 cgagcacggc gttgaagcgg tagtcaccgg cgccggcccc accgaactcc tcagggatat
     3961 ccatgccgaa gaagccctgc ttgccggcct cgagccagat gtcgcgggga atcgacttga
     4021 ggctgatcat ttcctcgacg cggggcttga gcgagcgctc cacgaactcc cggacgctgg
     4081 cccggaagtc ctcgtgctcc tcggaataca ggttgcgcgg catgagtgac ctccagatgc
     4141 gggtccgacg atggacgtaa gtaagcgctt gcttagcatg acataggtgg ggcatgctgt
     4201 ccactgtgtc gaccactcct gcggcggtga ccccagccag cgagaccgtg cagcgactgt
     4261 tctccgctgc ggcggaagcc ttcgccgcca agggcttcca cgccaccacg acccgcgaca
     4321 ttgcctcccg cgccggtttg tcacccgccg gggtctacgt gcacttcgcg tccaaggaag
     4381 atctcctctt ccacctctcc cgagatggcc acgaggaagc cctcgcgctg gtcaccgagg
     4441 cggttgctgg cgccgactcc cccgccgagg cgctcgccgc gacgatgtcc cgcttcagcg
     4501 cctggcacgc cgagaactac gcgatcgccc gcgtcgttca gcacgagttc ccgcacctgt
     4561 ccgacgccca ccgagccgag gtcctggccc tgcgcaagcg gatcgaccag gccgtgcgcg
     4621 acgttctcgc ggcaggcaag cagtccggcg acttcctgtt ggacgacatc cccgacacgg
     4681 ccctcgcgct cctgtcgatc atcgtcgacg tcgcccggtg gtacaccccc gcgttgcacc
     4741 gaacgtcggc tcagatcggt gcgacgaacg ccgccctcgg gctgcgcctg gtcggctacc
     4801 gcggcggcca gatcaaccag tcaggatccc gagcaatcca ctgagggtga ggaaggacac
     4861 gccggtcgtc accagcagca ccccggcgat gggttcgctc tcgccgtacg ggtgggccag
     4921 caccggcatg gtcgacatga tcggaagcgc cccggtgacg agcaacccga tgcgcagggc
     4981 cgggtcgagc gccggcagtc ccaaacccac gaggacgagc agggcccccg ccacgagcag
     5041 cggatgcagg aggagtttgc ctaggctcag tgcgagcacc cgacgaagtt cggcgccccg
     5101 cgggaaggtc gccaacagcc ctccgatggc gaacagcgcg acggccgttc cagccctgga
     5161 gaacaggtca gccgtgcgca ggaccgcctc aggcaggtgc accccggaga gcgagacgat
     5221 cagcccggcg accaggccga tgagcaacgg gttgcgggtc aatcgggtcg cgtaagtggc
     5281 caacaccttg gctcgggaat cgtgtccctg agcgcgccct tcggcgagca gaaacatcag
     5341 cggcagcacg aggaggttct cgacgatcat gctcaacccc aggaccagcc cagcgacgtc
     5401 gggcagcgtg agaagcagca gcggatagcc cacgaaaccg ctgttactcc cggacatgcc
     5461 cagcccgtcg aaggccctcc ccgccggacc ggcgccgcgc agccgcccat acccgtagcc
     5521 cacggcgtag gccaggaccg atcccagcgc gtatgccgcg aggtacgtcg gattgaggat
     5581 gtctctgacc gggcgggtcc cgatggcgag gaacagcatg cacggcaacg agatcgagat
     5641 gacgaacgtc gtaaggactc gcatgtccga gcggctcagc agaccccgcc gcacggccac
     5701 gtgcccgacc gcgacacagg catagatcgg gatggtgatg gccaggaccg aaagcatccc
     5761 ggccaggcta ttgagtcagc ggcccgccgg gacgatcggg gccacctgcc ggacgagctc
     5821 ggccacctgg tcgaacgccc caggtgtggc gacgttggcg aacccgagga tgacgtggtc
     5881 ggtaccgatc tcggcgagtc ggcccatccg ctcgaccccc tcggcgaccg acaccgtcgg
     5941 cgtcccggtg gcatccggcg aaccgtcccc gagggtcagc cggctgagca cggtcttctc
     6001 gatgtcggcg taattgcgtc cgagcgcgtc gcagtgtccg cgcagcacgt cgtacttcgc
     6061 ggtaatcgcc gccgggccca tgtcgaagat gttgcaggcg tcggcgtact gcgcgaccag
     6121 tcgcaacgtc ttcttctcgc cgccgccgcc gatgaggatc ggcgggtgcg gtcgggtcag
     6181 cgactgcggg acgttgaggg gccgggtgag ttgatagtgc tgaccggcat acgggctctc
     6241 gtcacctcgc cacatctgca gggcgatctg cagggtctcc tcaagccttt cgaagcgctc
     6301 ggcgagcgcc ggatacggaa cgccgaggcc gaggtgttcc tcctcgttcc aggccgcgcc
     6361 gatgcccagc cacgcgcgcc ctccggagag cacgtccagc gtcgtgacgg tcttggcgag
     6421 cacgccgggg tagcgatagg tcacgccggt gaccatggtg ccgagctgga tccgctgggt
     6481 ttggccggcc gcgaacgcga gtgcggtgta gccctcgagc atgtcgagct cggccggccc
     6541 gatcatcgag atctggaaga agtggtccat cacccacagc gagtccatcc ccgcggcatc
     6601 ggcgtcctgc gcgatccggc cgaaggtggg gccgatcgac gccggggcgt cgggccaggt
     6661 gaagtaggag atctgggttc caaggcgcat ggggacagtc tgcgggtaac cggcaggcgc
     6721 gcataacggg ttgcccgaag accggccggc cccttaggtc acggggaaag aggcacgggg
     6781 aaaggatggt gggatggtgg cgtgtccacc actgcgaccc cgcctcccgc ggcccagcct
     6841 cccgcccgcc gccgaaccgg ccgtttcggg gagaaggaat gggaggtcta cgacgcctgg
     6901 accccagtca cgctgcgcgg tgacgtgacg gtcgtcctgg tccacggcgg cctgtggcag
     6961 gcggcatacg accgggccca cctgcgcccg ctgggagcgg cgctggcacg ggacggatgg
     7021 cccgtcgcct ccgtggaata cacccgcgtc ggtatgccgc gcggcggctg gccgggcacg
     7081 gccgagtccg tcctggccgc catcgacgcc gtcgctgctg accccgagct gcccgaccgg
     7141 cttgtcgcgg tgggacattc ggctggcggc cacctcgcgg tgtgggcggc gagcaatggc
     7201 cgttgccctg ccctgaccgg tgtggtgtcg cttgccgggg tgctcgacct gcgcctggcc
     7261 gacaccgacg atctcggctc tggtgcggtc cgggccttgc tgggtgggga gcccacggag
     7321 tgcccgcagg cgtgggtcga ggccgatccg atggccgccc gcctgcacgt cccagcggtc
     7381 ctcctgcacg ggcgcagcga cgacctggtc ccctcggagg tctcggcgtc ctacctgcgc
     7441 tcccggggtc ccctggatgc cccttgccgg ttggagctcg tcccggcctg cgaccacttc
     7501 gggatcatcg acccgaggca cgcggcatac gagcgggtgc tggccgcgat cggggaggtg
     7561 gctggcacct ccccggcagg agagccgagg tcatgactgg cccggtctcc cccaccggac
     7621 cgcccaccac ggccggacac agcgtgctcg tcgtgcccat tcctgcactg gaaacattcg
     7681 tggcggcccg gcacgcgcac tacgaccgcg actacgtctc ggctgatccg accttcgcgc
     7741 atgcccacgt gacgatcctc ggcccctggc tgacccaggc cgacctggcc gacccggtcc
     7801 gggcgaacgc ggcgctcacc gcagtccgcg acatcgcttc ggcgacaagg ggattcgaca
     7861 tcgacttcgc gcacgtggac acgttcccga acgggatcat ccacctcgtt cccgagccag
     7921 ccgaaccgtt cgccgcgctc accgccgctg tgtatcgcga gttcccggca taccccccgt
     7981 atgccggtca gttcggcgtg gtgcagccgc acctcacgct cgacgccgtg ggcgcgggag
     8041 tcaccgaagc cgtcgttcga gggtgggtcg ccgacctgtt gccgctgcgg gtgcgcgccg
     8101 atcgactgca cgtgtcctgg tacgaagcgg gccggtgtca gaccctggca gtgttcccgt
     8161 tcgggcgctg accgcacccg acgcgcaggg ctgccgactc gtcagtcgcg ggtgagcttg
     8221 cggtaggtga cccggtgggg acgcgtcgcc tcgatgccca gtcgctcgac cttgttggct
     8281 tcgtaggcct cgaagttccc ctcgaaccaa taccacttgg ccgggtcttc ctcgtcgcct
     8341 tcataggcca ggatgtgggt cgcgacccgg tcgaggaacc accggtcgtg gctgatgacg
     8401 acggcgcagc cggggaattc gagcagggcg ttctccagcg agcccaacgt ttcgacgtcg
     8461 aggtcgttgg acggctcgtc gaggagcagg aggttgccgc cctgcttgag ggtgagcgcg
     8521 aggttgagcc ggttgcgctc gccaccggac aacaccccgg caggcttctg ctggtcaggg
     8581 cccttgaacc cgaactgcga gacataggcc cgcgacggga tctcgacctg gccgaccttg
     8641 atgaagtcca gcccgtcgga aacgacctcc cagagggtct taacggggtc gagcccggag
     8701 cggccctggt cgacatagga gatctgcacc gtctcgccga ccttgacctt gccagagtcc
     8761 ggcgcctcca gaccgacgat ggtcttgaac aacgtcgtct tgccgacgcc gttggggccg
     8821 atgacgccga caatcccgtt gcggggcaac gtaaacgaca ggttgtcgat gagcacgcgg
     8881 tcgccgaaac ccttgcgaag tttgcggacc tcgatgacct gcgaccccag gcgcgggccc
     8941 ggcgggatgg caagctcctc gaagtccagc ttgcgggtcc ggtcggcctc ggccgccatc
     9001 tcctcgtatc gagccagtcg ggccttgctc ttggtctggc gacccttggc gttggagcgg
     9061 acccactcca gctcgtcgcg cagccgcttg gcgagcttga cgtccttgcg gccctggatc
     9121 tgcaggcgct cggccttctt ctccaggtag gtcgagtagt tgccctcata gggatacagc
     9181 cggccgcggt cgacctcggc gatccactgc gcgacgttgt cgaggaagta ccggtcgtgg
     9241 gtgaccgcca acaccgcacc ggggtattgc gcgagatgct gctccagcca ctgcaccgac
     9301 tcggcgtcca ggtggttggt cggctcatca agcaacagca ggtcaggctt ctgcagcaac
     9361 aacttgcaga gggcgacccg acgccgctca ccaccggaga gcacggtcac gtcagcgtcg
     9421 cccggcgggc accgaagcgc atccatggcc tgctcaagct gactgtccag gtcccacgcg
     9481 ttggcatggt cgatctcgtc ctggagcttg gccatctcgt cgccaagcac ctcgtagtcg
     9541 gcgtccggct cacccatctc cagggcgatg gcgttgtagc ggtcgagctt ggccttgatc
     9601 tcgcccgcgc cctcctcgac gttgcccagc accgtcttgg actcgttgag cggcggctcc
     9661 tgcaggagga tccccacggt gtagccagga gagagccgag cctcgccgtt ggacggttgg
     9721 tcgagccccg ccatgatccg caggatcgtc gattttcccg cgccgttagg cccgacgacg
     9781 ccgatcttgg cggtcgggaa gaacgacatc gtcacgtcat cgaggatgag cttgtcgccc
     9841 accgccttgc gcgccttggt catggtgtag atgaactcgg ccattcccga aggctatccg
     9901 tcacgcacct gccgcaccca atcgccggtc gggccgcggg ggtggatcgc gaaggttgtc
     9961 acgacccgac cggctgcctg gtgggtatgc cgtcagcggg cgtgcgcgac gacgtaggcg
    10021 tcggtctcgg gattggcacc ggaccacgac gaagcgcccg tcaggggcgc gccctcgatg
    10081 acgaccgggt ccgcggcgtc cgcggtgaag tcgatgttga tcggggacca gtccggttca
    10141 cggtcctcat cctcgatgtc ggacggtccg tccacgtcct ccgccaacgc ctgctggatc
    10201 tccggctcgg agagtcggtc ggtcgtgtcg aactgggccc gcggcgactt ggtgaacagc
    10261 gactggcccc acatgaggtc atgcccgacg tggtaggcgt cgatctccac cgacgtgttg
    10321 gagtggccct cggcggtgct ccacatgttg acccgcagcc gcccgtagac gatgacgggc
    10381 tgccccttgg ccatgctcgc cacgacgttg gcggccaacg agttgaaggt caccaccgac
    10441 acgaagttgg tgccgccatc gacataggct cgctcgcggc cgtcccaccg gcgcggggtc
    10501 gacgccatgc ggaaggtcgc gaaaggcttg ccggccttgg tggtgcgcaa caccgggtcg
    10561 gcggtcagat tgccgacgac ggtcacgtag ctctcgtaca tgtgggtgtc ctctacctgg
    10621 agggggtatg ccgccccgtc cattgcgaac gggctaggca cagcctctcc ggggaaggcg
    10681 gcgacgcggc ataccccagc gccagcctgt ggacgatggg acgaacgacc acgggggtgt
    10741 ggacaacacc cac
//
LOCUS       DAOMZY010000087        10703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5775_length_10703_cov_5.637303, whole genome shotgun sequence.
ACCESSION   DAOMZY010000087 DAOMZY010000000
VERSION     DAOMZY010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 10703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10703
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5775_length_10703_cov_5.637303"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PLK64_06625"
     CDS             <1..445
                     /locus_tag="PLK64_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HPV79452.1"
                     /translation="LNEQVVMRRTETTNNPANIALQPRMTSVNTALQVDLFGQANASR
                     IKARIHSGFGGQTDFIVGALHSPGGQAIMALRSWHPKADVSTIVPLIEEPVTSFQHSA
                     IITEQGVAEIFGHDQAHQCWSIINKAAHPSVRDELWEEAHALGLA"
     gene            463..810
                     /locus_tag="PLK64_06630"
     CDS             463..810
                     /locus_tag="PLK64_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79453.1"
                     /translation="MSGVSGRAPTRDEWRVFGWWALVGLGSIWATWTLLSYGWIIAVI
                     TCAGALMLANREEPGRSAWGFVTGMGLVPLWIGWTNRAATTAGLSPSVWIGSGAVTAL
                     VGVLIFVRPRRTT"
     gene            820..2013
                     /gene="coaE"
                     /locus_tag="PLK64_06635"
     CDS             820..2013
                     /gene="coaE"
                     /locus_tag="PLK64_06635"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPV79454.1"
                     /translation="MLRVGLTGGIGSGKSTVAALLREWGAVVTDADRVAREIVEPGMP
                     PLAAIRERFGPSVIQADGTLDRARLAAVVFPDPEALRALEAITGPAIADRVAQLRGSV
                     PVDRIDVYDMPLLVEKGLWVHEHLTIVAGADAEVRVARLVGQRGLSESDARARIAAQA
                     SDIQRHAAADAWIDNGGSREDTQAQVRRLWDARLVPFDANLRGGIRARRSDTTTLAEP
                     DPGWADAGARVVARIAAALPGQGIRVDHIGSTSVPGLVAKDVVDVQVGVPQLADADAP
                     GFAAAVRQAGYLLVVGNDQDHPHPTGAPPAQWQKRFYGGCDPGRIVHVHVREIGSAGW
                     EFALSFRDWLRAEPSAREEYAALKRELAARHPVTRDYSAAKEPWFDTAYLRVQEWARE
                     VRWTP"
     gene            complement(2027..2704)
                     /locus_tag="PLK64_06640"
     CDS             complement(2027..2704)
                     /locus_tag="PLK64_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPV79455.1"
                     /translation="MSVVFLHGTGSAGPKAWPLQAAALGDEAHFVRRVHDGDHPDHVQ
                     PVVIDALAPRGHLVAHSYGCVSAVLAATERPDVVASLVLCEPPFVGLTAGWPETDAHV
                     AALAPLFERRSDPTFTDLELSAGFATAVGMPVPDFTPDQLARSAARLRAFTPAWLIAI
                     DPTIVGRVPTLVVTSGNRDIYDEVAYLLEGHGATRLVLSGHGHRVQDHPDATAAFRRF
                     WGLHGVG"
     gene            2795..4933
                     /gene="uvrB"
                     /locus_tag="PLK64_06645"
     CDS             2795..4933
                     /gene="uvrB"
                     /locus_tag="PLK64_06645"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPV79456.1"
                     /translation="MRPTTDLQRRVAPFEVISDFEPSGDQPDAIAELARRIRAGVTDT
                     VLLGATGTGKSATTAWLIEQVQRPTLVMAPNKTLAAQLANEFRELLPNNAVEYFVSYY
                     DYYQPEAYIPQTDTYIEKDSSVNDEVERLRHSATNSLLTRRDVIVVASVSCIYGLGTP
                     QEYIDRMVALKVGQEIERDQLLRRFVGMQYTRNDLAFTRGTFRVRGDTVEIIPMYEEL
                     AVRVEFFGDEIERIYTLHPLTGEVVREEQEMYVFPASHYVAGPERMERAIGGIESELE
                     TRLAELDRQGKLLEAQRLRMRTGYDIEMMRTLGFCNGIENYSRHIDGREAGSAPNCLL
                     DYFPEDFLLVIDESHQTVPQIGAMYEGDMSRKRMLVDHGFRLPSAMDNRPLKWEEFLE
                     RIGQTVYLSATPGDYEMAKSRSFGPPVEQIIRPTGLIDPEIILKPTKGQIDDLIHEIR
                     TRAARNERVLVTTLTKKMAEDLTDYLLDKGIRVRYLHSEVDTLRRIELLRELRLGDYD
                     VLVGINLLREGLDLPEVSLVSILDADKEGFLRSTRSLIQTIGRAARHVSGQVHMYADK
                     LTPSMRDAIDETNRRRVKQVAYNTERGLDPQPLRKKIADITDLLGRESADTEELLGSG
                     RSRSRGKASGMPAGRDRAGRGAILADVGVAGGKRVEGLPTSDLAGLIQELTAQMHTAA
                     AELHFELAARLRDEVSELKKELRQMTEALR"
     gene            5009..5332
                     /locus_tag="PLK64_06650"
     CDS             5009..5332
                     /locus_tag="PLK64_06650"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HPV79457.1"
                     /translation="MTTSEVSLAAWPIGAPGVLASAAAPAGPPSVAAPTDLLTHLARL
                     GLRLGMTVTPLMRTPGRGLVLAVGDLRLAVDRASADALRVHAPGHSSPRSTSNTPDPT
                     PTVEV"
     gene            5334..>6063
                     /locus_tag="PLK64_06655"
     CDS             5334..>6063
                     /locus_tag="PLK64_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HPV79458.1"
                     /translation="MGNAGLETGGAVTAAPTPSRHAPAAPQAPTGSLVIALAGAPNVG
                     KSTLFNALTGARLTMGNWPGTTVEVSRGRWQPDAGSVTYELVDLPGAYSLDPISPDEE
                     LTRAVLLDPDPAARPHVVIVAADATHLARSLYVVAQLREHAQRIIVALTMVDVAARRG
                     ITVDPGGLERALGIPVVAVDPRRRTGLSALAERVTQIAGAPEPSPRPHDTDTRGDRDG
                     DSDELDRAEERFGWVQDAVDRAQTR"
     assembly_gap    6064..6163
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6164..7486
                     /gene="feoB"
                     /locus_tag="PLK64_06660"
     CDS             <6164..7486
                     /gene="feoB"
                     /locus_tag="PLK64_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HPV79459.1"
                     /translation="AEERFGWVQDAVDRAQTREAAARHTWTDRVDAVVLSRVWGPIVF
                     LAVMWLVFQATTTVAAPIQDALDTLFTGPLSEAAWAALAALHVDHPLVTGLVVDGLIA
                     GVGMLLTFVPLMAVMFVLLALLEDSGYLARAAVVTDRTMRALGLPGRAFLPLIVGFGC
                     NVPAIAATRVLGGAKQRVLTALLVPFTSCSARLTVYVLLATTFFDRYAGTVVFGMYLL
                     SILLIVGVGLVLRRTLWRSMLAEPLLLDLPPYQRPTARVTWSVTWTRLRGFLRTAGGI
                     IVATVAVVWLLQSVPVRGEHAFGATPVGDSAYAALAGAVAPIFEPAGFGQWQSAGALV
                     TGFVAKEAVISSWAQTYATLDDTGAADEGRLAQAVRQDFEISSGGHGNAAALAFMVFL
                     LAYTPCVATLAAQKREIGVRWAAFGVGMQLAVAWVLSVVVFQIARLVG"
     gene            7483..7803
                     /locus_tag="PLK64_06665"
     CDS             7483..7803
                     /locus_tag="PLK64_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoC-like transcriptional regulator"
                     /protein_id="HPV79460.1"
                     /translation="MTLPSQARAKPLHLVLAAIGSGVGSRGELTRHTGLAPDVVDAAV
                     DHLVSTGRLTNTALGGGCATGGCGACPSGRADAGPGCGVADPRDARGPVVLSLATLAT
                     RSRG"
     gene            complement(7810..8847)
                     /locus_tag="PLK64_06670"
     CDS             complement(7810..8847)
                     /locus_tag="PLK64_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HPV79461.1"
                     /translation="MDVTPLVWWVTIGAATVALLLDVAVVARRPHVPSMRECTTYLVV
                     YVSLALLFGLFIWWDFGGQYAGEFYAGWLTEYSLSVDNLFVFLLIMANFRVPERLQQS
                     ALMVGIIIAIVLRGLFIALGAAVINSYAWVFYIFGAFLIWTAWKLAREGESDDDEYEE
                     NAFLQFVERRFPATSSFHGTKLFVHEGAKRLVTPMFIVIMALGTTDLLFALDSIPAIY
                     GLTKEPYLVLTANIFALMGLRQLYFLIGGLLKRLVYLSIGLAVLLAFIGVKLILHALH
                     ENNLPFINGGQPLHGIPDIPISVSLGAIVLILGVTTIASLLKSSRDRKAAERTAGQAI
                     TDPTEDDHPDW"
     gene            complement(8881..9225)
                     /locus_tag="PLK64_06675"
     CDS             complement(8881..9225)
                     /locus_tag="PLK64_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HPV79462.1"
                     /translation="MTREELLAACLAKPGAWPDEPWDGDVVAKVGPKIFAFTGEDAVG
                     LKLGRGRDEADEWLHRYPQDASVMAYIGRYGWNALRLAGAIPDDELLEAIDASYESVV
                     TALPRKQRPNAG"
     gene            complement(9241..10002)
                     /locus_tag="PLK64_06680"
     CDS             complement(9241..10002)
                     /locus_tag="PLK64_06680"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HPV79463.1"
                     /translation="MALLRVSVPVTTLWTSPDAPRPVDAAAIADQADPARWLAVLDAR
                     PQDDESGDGRLGLHGRVESQAVFGEPVLPISDVSADGWVRVVCPWQPSRLDPCGYPGW
                     VRRSHLEDPGSAADEPGLPVADAPGADVLTLAAEHLGLPYLWGGTSPLGLDCSGLVHH
                     VWRRLGVVVPRDADDQEAAAYPVAFGEERPGDLYFFAHPGRAVHHVGIVSAPGRMLHA
                     PETGRGIVEEALGPEREATRYAVGRLPLPGTAQRS"
     gene            10036..>10703
                     /locus_tag="PLK64_06685"
     CDS             10036..>10703
                     /locus_tag="PLK64_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HPV79464.1"
                     /translation="MDPAAVTARLADLVAAAPGRLSVAVRARDGYRFDHDSARVVPAA
                     STIKVPVLLAYLASGMPWDLPVALPAARVGGCGPLALLPSVTSLPAGEALRLMIALSD
                     NDATNAVLGLVGVGEVARLLGRAGTRHTVLRRRMMDQVAAAAGHENETCAADLAELLA
                     AVRAGSILPQREAAMALAVLREQQFDEGLPAYLPVGLRCASKTGSLPGVRHDIAVIES
                     GDAWV"
BASE COUNT         1618 a         3713 c         3659 g         1613 t
ORIGIN      
        1 cctcaacgag caggtcgtca tgcgccgcac cgagacgacc aacaacccgg ccaacatcgc
       61 gctgcagccg cggatgacat cggtcaacac cgcgcttcag gtcgacctct tcggccaggc
      121 caacgcatct cgcatcaagg cacgcatcca ctccggcttc ggcgggcaga ccgacttcat
      181 cgtcggcgcc ctgcactcgc ccggcgggca ggcgatcatg gcgctgcgtt cctggcaccc
      241 caaggccgac gtctcgacga tcgtgcccct catcgaagag ccagtgacct ccttccagca
      301 cagcgcgatc atcaccgagc agggcgtggc cgagatcttc ggtcatgacc aggcgcacca
      361 gtgctggtcg atcatcaaca aggcagccca cccgagcgtg cgcgacgagc tttgggaaga
      421 agcgcacgcc ctgggcctgg cctgagcggt cgggggagcg gcgtgagtgg cgtgagcggt
      481 cgggcgccga ctcgcgacga gtggcgggtc ttcggctggt gggcgctggt cggcctcggg
      541 tcgatctggg ccacgtggac gctgctgagt tatggctgga tcatcgcggt gatcacctgc
      601 gccggagcac tcatgcttgc caaccgcgag gaaccggggc gctcggcgtg gggtttcgtc
      661 accggcatgg ggctggtccc gttgtggatc ggctggacca accgggccgc cacgacagcg
      721 gggctctcgc cgtcggtctg gatcgggtca ggggccgtca ccgccctggt tggagtgctc
      781 atcttcgtcc gccctcgccg cacgacttag gctgacgcca tgctgcgcgt ggggctcacc
      841 ggggggatcg gctcgggcaa gtccactgtc gcggccctcc tgcgcgagtg gggggcagtt
      901 gtgaccgacg ccgaccgggt ggcgcgcgag atcgtcgaac caggtatgcc gcccctggcg
      961 gcaatccgag aacgcttcgg tcccagcgtg atccaggctg acggcaccct cgaccgggcg
     1021 aggttggcgg ctgttgtctt tcccgatccc gaggcactgc gggcgttgga ggcgatcacg
     1081 gggccggcca tcgcggaccg ggtcgcacag cttcggggga gtgtccctgt cgatcggatc
     1141 gacgtctacg acatgccgtt gctcgtcgag aagggcctgt gggtccacga acacctgacg
     1201 atcgtcgccg gtgccgacgc cgaggttcgg gtggcgcggt tggtcggcca acgggggctg
     1261 tccgagtcgg acgctcgggc gcggatcgcc gcccaagcga gcgacattca gcgtcatgcg
     1321 gcagccgatg cctggatcga caacgggggt tcccgggagg acacccaggc ccaggtgcgc
     1381 cgactctggg acgcccggtt ggtgcccttc gatgccaacc tgcgcggcgg catacgggct
     1441 cggcgttcgg acaccactac gttggccgag ccggatcccg ggtgggccga cgctggcgcg
     1501 cgcgtcgttg cgcggattgc ggccgccttg ccgggccagg ggatccgcgt cgatcacatc
     1561 gggtcgacga gcgtgccggg actcgtggcc aaggatgttg tcgatgtcca ggtgggtgtc
     1621 ccgcagttgg cggacgccga cgcgcccggc ttcgccgctg ctgtgcgtca ggccggctat
     1681 ctcctggtcg tcggcaacga ccaggaccac ccgcacccga ctggcgcccc gccggctcag
     1741 tggcagaagc ggttctacgg cggctgcgat ccggggcgga tcgttcacgt gcacgtccgc
     1801 gagatcggct ctgcgggttg ggagttcgcg ctgtccttcc gcgactggct gcgcgccgag
     1861 cccagcgcgc gcgaggagta tgccgccctc aaacgggagt tggccgctcg ccatccggtg
     1921 acgcgggact actccgcggc caaggagccg tggttcgaca cggcatacct gcgagtccag
     1981 gagtgggcgc gagaggtgcg ctggacgccc tagcgctcac tgggcctcag ccgacgccgt
     2041 ggagtcccca gaaccgccgg aaggcagccg tcgcgtctgg gtggtcctgc acccgatggc
     2101 catggccgga caggacgagt cgcgtggcgc catgtccctc caacaggtag gccacctcgt
     2161 catagatgtc ccggtttccg ctggtgacca ccaacgtcgg gacccgaccg acgatcgtcg
     2221 ggtcgatggc aatgagccag gccggggtga acgcccgtaa ccgggccgcc gaccgagcca
     2281 actggtccgg cgtgaagtcc gggaccggca tacccacggc tgtggcgaaa cccgccgaga
     2341 gctccagatc ggtgaaggtc gggtcgctcc ggcgctcgaa gagcggcgcg agagcggcca
     2401 cgtgcgcgtc ggtctcgggc caccctgccg tcagcccgac gaacggcggc tcgcacagga
     2461 cgaggctcgc gacgacgtcc ggccgctcgg tggcggcgag cacggccgac acgcagccgt
     2521 aggagtgggc gacgaggtgg cctcggggcg ccagcgcgtc gatgacgacc ggctggacgt
     2581 ggtcgggatg gtcaccatcg tggacccggc ggacgaagtg cgcctcgtcg ccgagggccg
     2641 ccgcctgcag gggccaggcc ttgggacccg cagatccggt tccgtggagg aagacgacag
     2701 acacggcccg agtatgccgt gcgcgcccta gggagcggca tacccggtgc gcgcgtgggg
     2761 tggggtgtcg gtggggcgac gtaccctgct cactatgcgt ccgacaaccg atctgcagcg
     2821 gcgagttgcc ccgttcgagg tgatctccga cttcgagccc agcggcgacc aacccgatgc
     2881 cattgccgag ttggcccggc ggatccgggc cggggtcacc gacaccgtgc tcctcggggc
     2941 gacgggcacg ggcaagtcgg cgacgaccgc gtggctcatt gagcaggtgc agcgacccac
     3001 cctggtgatg gctcccaaca agaccctcgc cgcgcagttg gccaacgagt tccgcgagtt
     3061 gttgcccaac aacgccgtcg aatacttcgt cagctactac gactactacc agcccgaggc
     3121 ctacatcccg cagacggaca cctacatcga gaaggactcg tcggtcaacg atgaggtcga
     3181 gcggctgcgg cactcggcga ccaactcgct gctgacccgc cgtgacgtca tcgtcgtggc
     3241 gtcggtgtcc tgcatctacg gcctgggcac cccgcaggag tacatcgacc ggatggtggc
     3301 gctcaaggtc gggcaggaga tcgagcgcga tcagttgctg cgccggttcg tcggcatgca
     3361 atacacccgc aacgacctgg ccttcacccg agggaccttc cgggtgcggg gcgacaccgt
     3421 cgagatcatc ccgatgtacg aagagctcgc ggtgcgcgtg gagttcttcg gcgacgagat
     3481 cgagcggatc tacaccctgc acccgctcac cggcgaggtc gtgcgcgagg agcaggagat
     3541 gtatgtcttc ccggcctcgc actatgtcgc cgggcccgag cgcatggagc gggcgatcgg
     3601 cggcatcgag tcggagttgg agacccggtt ggccgagctc gaccggcagg gcaagctcct
     3661 agaggcccag cgcctgcgga tgcgcaccgg ctacgacatc gagatgatgc gcaccctcgg
     3721 gttctgtaac ggcatcgaga actacagccg gcacatcgac ggccgagagg ccgggtcggc
     3781 ccccaactgc ctactcgact acttccccga agacttcctg ctcgtcatcg acgagtcgca
     3841 ccagaccgtg ccgcagatcg gggcgatgta cgagggcgac atgtcccgca agcggatgct
     3901 cgtcgaccac ggcttccggc tgccctcggc gatggacaac cgacccctga agtgggagga
     3961 gttccttgag cgcatcggcc aaaccgtcta tctgtcagcg actcccggcg actacgagat
     4021 ggccaagtcc cggtcgttcg gccctccggt ggagcagatc atccgaccca ccgggctgat
     4081 cgaccccgag atcatcctca agcccaccaa gggtcagatc gacgacctca tccacgagat
     4141 ccgcacccgg gcggcccgca acgagcgggt gctcgtcacc accttgacca agaagatggc
     4201 cgaggacctc accgactacc tgctcgacaa gggcatccgg gtgcgctact tgcactccga
     4261 ggtcgacacc ctgcgccgaa tcgagttgct ccgtgagctg cggctgggcg actacgacgt
     4321 cctcgtcggc atcaacctcc tccgcgaggg cctggacctc cccgaggtgt cgctcgtgtc
     4381 gatcctcgac gccgataagg agggtttcct gcgctccacc cgttccctca ttcagaccat
     4441 cggccgagcg gcccgccacg tcagcggcca ggtccatatg tatgccgaca agctcacccc
     4501 ctcgatgcgc gatgccatcg acgagaccaa ccggcgacgg gtcaagcagg tcgcctacaa
     4561 cactgagcgc ggcctggacc cccagccgct acgcaaaaag atcgccgaca tcaccgacct
     4621 gcttggtcgg gagagtgccg acaccgagga gttgctcggc tccggccgct cgcgctcccg
     4681 cggcaaggca tcgggtatgc cggccggccg cgaccgcgcg ggccgcggcg cgatcctcgc
     4741 ggacgtcggc gtggcgggtg gcaagcgggt cgagggcctg cccacctccg acctggccgg
     4801 gctcatccag gagctcacgg cccagatgca cacggccgcg gccgagttgc acttcgagct
     4861 cgcagcccgg ttgcgcgacg aggtctcgga gttgaagaag gaactgcgcc agatgaccga
     4921 ggcgctgcgc tgacgacgag agcaacctca cgctccgttc tcggccccca agtaaggcac
     4981 ccctaacttc gctcgatacc atgcgagggt gaccacgagc gaggtgtccc tggcggcctg
     5041 gccgatcggc gccccaggcg tgctcgcgtc agccgccgcc ccagcgggtc ctccctccgt
     5101 cgccgccccc actgacctgc tgacccatct ggcgagactg gggctgcgcc taggcatgac
     5161 cgtcactcca ctcatgcgca cccctgggcg cgggctggtt ctcgcggtag gcgatctacg
     5221 cctcgcggtc gatcgggcca gcgccgacgc cctgcgtgtg cacgcacccg ggcactcaag
     5281 cccgaggtcg acgtccaaca cccccgatcc gacgccgacg gtcgaggtct gacatgggca
     5341 acgccgggct tgagaccggg ggagcagtga ccgccgcacc caccccctcc cgccatgccc
     5401 cggccgcccc ccaagccccc acgggctcgc tcgtcatcgc cctcgcgggt gcccccaatg
     5461 ttggcaagtc gaccctgttc aacgccctca ccggagcgcg gctgacgatg ggcaactggc
     5521 cggggaccac cgtcgaggtg tcccgcggcc ggtggcaacc ggacgctgga tcggtgacct
     5581 acgagttggt ggacctcccc ggcgcctact ccctcgaccc gatctcgccg gatgaggagc
     5641 tcacccgcgc ggtcctgctc gaccccgacc cggccgcccg gccacacgtc gtcatcgtcg
     5701 ccgccgatgc gacgcacctg gcccgcagcc tctacgtcgt cgcccaactg cgtgagcacg
     5761 cccaacgcat catcgtggcg ctgaccatgg tcgacgtcgc ggctcggcgc ggcatcacgg
     5821 tggaccccgg cggcctcgaa cgcgccctcg gcatcccggt cgtggccgtc gaccctcgcc
     5881 gacgcaccgg cctgtcggcc ctcgccgaac gcgtgaccca gatcgccggg gcgcccgagc
     5941 cgtcgccgcg cccgcacgac accgacaccc gcggcgaccg ggacggcgac agcgacgaac
     6001 tcgaccgcgc cgaggaacgc ttcggttggg tccaggacgc ggtggatcgg gcccagaccc
     6061 gagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngccgagg aacgcttcgg
     6181 ttgggtccag gacgcggtgg atcgggccca gacccgagaa gccgctgcac ggcatacctg
     6241 gaccgaccgg gtcgacgcgg tcgtgctctc gcgcgtctgg gggccgatcg tcttccttgc
     6301 ggtgatgtgg ctcgtcttcc aggccacgac gaccgttgcg gccccgatcc aggacgccct
     6361 cgacaccctc ttcaccggcc cgctgagcga ggctgcgtgg gcagcgttgg cggccctgca
     6421 cgtcgaccac ccgttggtca cggggttggt ggtcgacggg ctcatcgctg gggtcgggat
     6481 gctgctcacc ttcgtcccac tcatggccgt gatgttcgtc ctgctggcac tgctcgagga
     6541 ttccggttac ctcgcccggg cggccgtcgt caccgatcgg acgatgcgcg ccctcggcct
     6601 gccggggcgg gccttcctgc cgctcatcgt cgggttcggc tgcaacgtgc cggccatcgc
     6661 ggccacccgg gtgctcggtg gagccaagca acgggtgctg accgctctgc tcgtcccctt
     6721 cacctcctgc tcggcccgcc tcaccgtcta cgtcctgctc gcgacgacgt tcttcgatcg
     6781 gtatgccggc accgtcgtct tcggcatgta cctcctgtcg atcctgctca tcgtcggcgt
     6841 cggcctcgtc ctgcgccgca ccctgtggcg gagcatgctc gccgaaccgc tcctgcttga
     6901 cctgccgccc tatcaacgac ctacggcccg ggttacctgg tcggtcacct ggacccggct
     6961 gcggggattc ctgcgcaccg ccggcggcat catcgtggcc acggtcgctg tcgtgtggct
     7021 cctgcagagc gtcccggtcc gcggcgagca cgccttcggg gcgactcccg tcggggacag
     7081 cgcgtatgcc gccctcgccg gcgccgtcgc gccgatcttc gagcccgccg ggttcgggca
     7141 atggcaatct gcgggggcgt tggtgaccgg gttcgtcgcc aaggaggctg tcatctcgtc
     7201 gtgggcgcag acctatgcga cgctcgacga cacgggtgcc gccgacgaag ggcgcctggc
     7261 gcaggcagtc cggcaagact tcgagatctc ctccgggggg catggcaacg cggccgccct
     7321 ggcgttcatg gtcttcctcc tggcctacac accttgcgtg gcgaccctgg cagcccagaa
     7381 gcgcgagatc ggcgtgcggt gggccgcgtt cggggtcggc atgcagttgg ccgtggcctg
     7441 ggtgctctct gtcgtggtct tccagatcgc gaggctggtg ggatgaccct gccgagtcaa
     7501 gccagggcca agccgctgca cctcgtcctg gccgccatcg gctccggggt cggcagccga
     7561 ggtgagttga cgcggcatac cggattggcg cctgacgtcg tcgatgccgc cgtggaccat
     7621 ctcgtgtcga ccgggcgatt gacaaacacg gcgctcgggg gcgggtgcgc caccggcggt
     7681 tgtggcgcgt gcccgagcgg gcgggccgat gccgggcccg ggtgtggcgt ggccgaccct
     7741 cgcgatgcgc gggggcccgt ggtgctcagc ctcgccaccc ttgccacgcg cagtcgcggc
     7801 tgagggacct caccagtccg ggtggtcgtc ctcggtgggg tcggtgatcg cctggccagc
     7861 cgtccgctca gcggccttgc gatcgcggga cgacttgagg aggctggcaa tggtcgtcac
     7921 gccgaggatg aggacgatgg cgcccaacga caccgagatc gggatgtcgg gtatgccgtg
     7981 caacggctga ccgccgttga tgaacggcag gttgttctcg tgcagcgcgt gcaggatcag
     8041 cttgacgccg atgaaggcca acagcacggc cagcccgatc gacaggtaga ccaggcgctt
     8101 gagcagcccg ccgatgagga agtagagctg acgcaggccc atgagggcga agatgttggc
     8161 ggtgaggacg aggtacggct ccttggtgag cccgtaaatc gcggggatcg agtcgagggc
     8221 gaagagcagg tcggtcgtcc ccaacgccat gatgacgatg aacatcggcg tcaccagccg
     8281 cttggcgccc tcgtggacga agagcttggt gccgtggaag gaactcgtcg ccgggaaccg
     8341 tcgctcaacg aactgcagga acgcgttctc ctcatattcg tcgtcgtcgc tctcaccctc
     8401 ccgggccaac ttccaggccg tccagatgag gaaggcgccg aagatgtaga acacccaggc
     8461 gtaggagttg atgaccgcgg ccccgagcgc gatgaacagc ccgcgcagca cgatggcgat
     8521 gatgatgccc accatgaggg ccgactgctg cagccgctcg ggaacccgga agttggccat
     8581 gatgagcagg aagacgaaga ggttgtccac cgacagggag tactcggtga gccagccggc
     8641 atagaactct ccggcgtact gcccgccgaa gtcccaccag atgaacagcc cgaacaacag
     8701 cgccagcgag acatagacga ccaggtaggt cgtgcactcg cgcatcgacg gcacatgggg
     8761 tcgacgggcc acgacggcga catcgagcaa cagggccact gttgccgcac cgatagtgac
     8821 ccaccacacc aggggagtga cgtccacgaa gcaccttccg ttggggatgt ccctgagact
     8881 ctacccagcg ttggggcgct gcttgcgcgg cagcgccgtc acgaccgact cgtaggacgc
     8941 gtcgatcgcc tcgaggagtt cgtcgtcagg gatggccccc gccaaccgca gggcgttcca
     9001 tccgtagcga ccgatatagg ccatgacgct ggcgtcctgc gggtagcggt gcagccactc
     9061 gtcggcctcg tcccgccctc gcccgagttt gagccccacc gcgtcctcgc cggtgaaagc
     9121 gaagatcttc ggaccgacct tggcgacgac atcgccgtcc catggctcgt ccggccaggc
     9181 gccgggcttg gccaggcaag cggcgagcaa ctcctcacgc gtcatggccg cgaggctaac
     9241 tcaggaccgc tgcgccgtgc cgggcagcgg cagccgtccc acggcatacc gggtcgcctc
     9301 gcgctccggc cccaacgcct cctcgacgat cccgcgcccc gtttcggggg catggagcat
     9361 ccgcccaggg gcgctgacga tccccacgtg gtggaccgcg cgccccgggt gggcgaagaa
     9421 gtagaggtca cccgggcgct cctcaccgaa cgccaccggg tatgccgcgg cctcctgatc
     9481 gtcggcgtcg cgcgggacga ccacgcccaa ccgacgccac acgtggtgca ccagccctga
     9541 gcagtccagc ccgagcggtg aggtccctcc ccagaggtag ggcaggccga ggtgttcagc
     9601 agcaagggtg aggacgtcgg ctccgggagc gtcggcgacc ggaaggccgg gctcgtcagc
     9661 ggccgagccg gggtcctcaa gatggctgcg gcgcacccag cccgggtatc cgcaggggtc
     9721 cagccggctg ggctgccacg ggcacaccac ccgaacccag ccgtcggcgc tcacgtcgct
     9781 gatcgggagg accggctcgc cgaacacggc ctgactctcg acccggccgt gcagaccgag
     9841 ccggccgtcg ccgctctcgt cgtcctgcgg gcgggcgtcg aggacggcca accacctggc
     9901 cgggtcggcc tggtcggcga tcgccgcggc atcgaccggc cggggggcat ccggcgaggt
     9961 ccagagggtc gtcacgggca cggacacacg cagcagggcc atcgccccat cctcacatgc
    10021 ccgatactgc cggtcatgga tcccgctgcg gtgaccgctc gtctcgccga tctcgtggcc
    10081 gcggccccag gccgtctgtc ggtcgccgtt cgtgctcggg acggctaccg gttcgaccac
    10141 gactcggcgc gagtcgtccc tgcggcgagc accatcaagg tgcccgtgtt gctggcctac
    10201 ctcgcctcgg ggatgccgtg ggacctgccc gtggcgctgc cggccgcccg ggtgggtggg
    10261 tgtggcccgc tcgcgctgct gccctcggtg accagcctcc ccgcggggga ggcgttgcgc
    10321 ctgatgatcg cgctgtccga caacgacgcc accaacgcgg tgctcgggct ggtcggtgtg
    10381 ggcgaggtgg ctcggttgct cggacgcgcg gggacgcggc ataccgtgct gcggcgccgg
    10441 atgatggacc aggtggcggc cgctgcgggt cacgagaacg agacctgcgc cgcggacctg
    10501 gccgagttgc tggccgccgt gcgcgccggg tcgatcctgc cccagcgcga ggccgccatg
    10561 gcgctggccg tcctgcgcga gcagcagttc gacgagggcc tgcccgccta ccttcctgtg
    10621 ggcctgcgct gcgcgtcgaa gaccggcagc cttcccgggg tgcgccacga catcgcggtc
    10681 atcgagagcg gtgacgcgtg ggt
//
LOCUS       DAOMZY010000088        10686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5787_length_10686_cov_5.858245, whole genome shotgun sequence.
ACCESSION   DAOMZY010000088 DAOMZY010000000
VERSION     DAOMZY010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 10686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10686
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5787_length_10686_cov_5.858245"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..531)
                     /locus_tag="PLK64_06690"
     CDS             complement(175..531)
                     /locus_tag="PLK64_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79465.1"
                     /translation="MILREVAYARSGDKGDTSNICVFVHEPADYPMLVERLTAERVRQ
                     HFAGLVRGEVVRYELPRVQGLNFVLFQALDGGVSATLRIDPHGKSFQSLALAMDVGDG
                     WSARPLGEASGTVGGA"
     gene            complement(528..1844)
                     /locus_tag="PLK64_06695"
     CDS             complement(528..1844)
                     /locus_tag="PLK64_06695"
                     /inference="COORDINATES: protein motif:HMM:NF018938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1446 domain-containing protein"
                     /protein_id="HPV79466.1"
                     /translation="MSIGAGSAYANDRLAPAAAMADSGRVTYIGFDSLAERTMALAHQ
                     RRRRDPATGQDERIRELVPLLAGHLGRGHRVVGNFGAANPDAAVADLVDSLRAQGQHG
                     IPIGVIRGDAVLDLVRSLDCALPERGTTVAALGDRVVSANAYIGADPIVTCLEAGAQI
                     VVGGRIADPSLFVGPICHELGWALDDWDAVAHATMVGHLLECGVHSTGGNFEDPPYRV
                     VPDPHRLGFPLADVTRADAIITKLPGTGGAVDARTMKTQLYYEIHDPAAYLTPDVTAD
                     FSDIEIDDLGGDRVRLWGARGRPRPETLKVLVGVDDGFRATGEISFGGPGCVTRARRA
                     EEVLRAYLQPYAASLTDVRVDLVGLTSLFGDRPTSPEPAEVRLRLAVHTPSRAVADAA
                     AHEVELLYFGPSGGGGVVTSVVPALGVTPAFVPRELVTLTTEVVRS"
     gene            complement(1877..2071)
                     /locus_tag="PLK64_06700"
     CDS             complement(1877..2071)
                     /locus_tag="PLK64_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HPV79467.1"
                     /translation="MKVQVNEERCQGHGLCRLSAPNFFFARVEDGHAEVRSEDVPDEE
                     REAVLLAAESCPELAIDVSD"
     gene            2185..3426
                     /locus_tag="PLK64_06705"
     CDS             2185..3426
                     /locus_tag="PLK64_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HPV79468.1"
                     /translation="MTETQRCPVVHFDHNSDEHSADPAGSYKRIRDEHPAAWSEAHGG
                     YWVLSGYRTVFDASRDDATFSSARNEHGGEGLSVVIPKTPMHFHIPIEVDPPDFRKWR
                     KLINPITAPAAVERMETMVRHYVTWFIDEIIEKGSCDMAEVIGVPSIVTVDWIGLDPA
                     DWKRYAFAHRMVLAAVPGSDDWKQAIEVEFPYINARTWEAIADRRINPTDDMISYFVQ
                     QEIDGRPITDDEVFSIVDLLLAGGVATTASLVSNTVVWLYQHQDVRQQLIDDPSLLDK
                     AIEEFLRFFSPTQALARTVTQDTEFHGCPMREGDRVLLSWASANRDPEQFEDPDDVNI
                     ERWPNRHTAFGIGVHRCAGSNLGRFMAKEMLSQILTRMGDYVIDLDSLQAYPHQGTNT
                     GWQRIPATYTPGPRILPVPAG"
     gene            3499..4743
                     /locus_tag="PLK64_06710"
     CDS             3499..4743
                     /locus_tag="PLK64_06710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HPV79469.1"
                     /translation="MNPHVVVVGAGLAGLRAAEQLRAAGHAGSIEVIGGEPHLPYNRP
                     PLTKQALVAGPDVMTLMFPRRPSIENVRWTLGRRAVSVDLDGSTLSLDDGSTRDFDGL
                     VIATGVRSRRLPIGEGGGPAPTLTLRTVDDALALHARLAPGARVVVIGAGFIGCEVAA
                     TVAARGGAVHVLDPLPAPLAGVLGPAVGSAVRARLEAAGVAFSLGVGVAQVLATPTGG
                     YAVRLTDSADVITADVVVEAVGSVPETEWLAGQGLDLGNGIGCDGDLLVTRGGIPLRN
                     VVALGDIARFPHPWFGQEIRVEHWAWPTDLARRAAASLVAGLGPTPAPDTPFTPLPSF
                     WSDLPGGRIQSFGLPPLGLGDVRLLEGEWEGDCAVGYHRDGRLVGLALVGLASRMIDY
                     RTALLDDLSTGTGAGADAGPTG"
     gene            4740..5972
                     /locus_tag="PLK64_06715"
     CDS             4740..5972
                     /locus_tag="PLK64_06715"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV79470.1"
                     /translation="MNRAGPRADPDPAPGGSARRAWALAALVTLFGLNLRATLGSIPP
                     LLGEIATDLGLGSSALGLLLSTCILAMGAFAPTGHLLATRVGTERALTLLLAILGVSE
                     VVRVAAYHPVVLYASVALAGATMGAASTLMPGFIAHHVPRTRGLTMGIYSTGLALGVA
                     LAAATAVPLERLLGGWRPALAVVGLLSLATAVLSWASEGTLVRSMPGHSVDPPRTRDP
                     LPWRSVPARWLTLLNALVMAIGFSGLAWVAPYYVDLGMSVQDAAGMVVLFQVVQLVAM
                     LALPGITDVTTDRRPLVGGMLLATLAGTVMLGVAPMAQPLLAVGLFGLGAGGMATLLL
                     VLSVDLTDSRSASASLNAMTMLVGYAVGAAGPFLLGAARDVTGSLGPGYLVVSGLVVV
                     SLVIVSVFRPGLKVARAD"
     gene            complement(5980..6552)
                     /locus_tag="PLK64_06720"
     CDS             complement(5980..6552)
                     /locus_tag="PLK64_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPV79471.1"
                     /translation="MAERSLDERIRRLEDRAAIQELGILYGFVMDERDGDGIREIFCE
                     DATLRSQDGVFKANGIEEIVETYLGRFAALGPTNHFSHGHVVILDDTDPDRATGLIAS
                     HAEVSRNGVAMQVALRYKDTYRRVDGRWRFADRLMSYMYYLPFTELSEGLGDRNSVRA
                     YGDHRPSDWPEVLYAETGNAWLAPYYTSRG"
     gene            complement(6603..6911)
                     /locus_tag="PLK64_06725"
     CDS             complement(6603..6911)
                     /locus_tag="PLK64_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79472.1"
                     /translation="MPKKRPHDRADLFMAPVLLDVDERISQLAQLDAQALADRVLVHV
                     NHETSDGAERRDALLATLTDGLELHGWKAKWHDRGLRLTHGEHSVVLGLGPELRAYLS
                     "
     gene            7004..8656
                     /locus_tag="PLK64_06730"
     CDS             7004..8656
                     /locus_tag="PLK64_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPV79473.1"
                     /translation="MPDDVLAHPESSGSATDVDVLVLGTGAGGLTAALAAATAGARVG
                     LYEKGERVGGTTALSSAVAWLPANRYAAQAGIADSREDALAYLSALSHGLIRPELAEA
                     FVDTVPELLDWLDEQTPLRMRLVAGFPDYHPEHPGGMPRGGRSLEPELFPFTEIPGWG
                     ERMVGVTRPMLVAETPIGGGSGLLPADVAAQRKAASLEGVGRGLVGALLKGCLDRGVE
                     PVTGARATRLVVTDGAVTGVEFDSGAERFVVTARSVILATGGFEHDLDLVRSFLRGPM
                     HLPAGVPTNTGDGLRMAMRVGAELGMMSEAWWVPVLALPQADPAATHRPVLLVNLERT
                     LPRSIMVNGQGRRFTNEAANYNALGGAFHHLDPTSFAYVNQPAWLIIDQGFVDRFGGF
                     GVPPGGPAQPWLIRADDLGALAEATGIPPTALSDTVVRWNEQAAALRDDDFHRGQSAY
                     DGWSGERRHYPGPAATLGPLDEPPFYAVEIVGSCLGTKGGPRTDVDGRVQHLDGGPIP
                     GLYAAGNAMAAPTGMVYGGAGGTLGPALVFGYRAGRSAAREC"
     gene            complement(8683..8988)
                     /locus_tag="PLK64_06735"
     CDS             complement(8683..8988)
                     /locus_tag="PLK64_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HPV79474.1"
                     /translation="MSHQPAELPVVDVHETGGCACAEHEEAVPELDARTIPHAIRHAA
                     IFGALDSVRPGHAMVLVAPHDPLPLLAQISARSGDAVEVSYLQRGPEAWRLRLHRVR"
     gene            complement(9032..9778)
                     /locus_tag="PLK64_06740"
     CDS             complement(9032..9778)
                     /locus_tag="PLK64_06740"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPV79475.1"
                     /translation="MLNTPYRSPVLGPAPTQEPTAVATPAQGEVLAALSADGPATAAE
                     LGARTGQHPNTVREHLDALVDLGLAARDRDAPAGRGRPAYRYAALPHPSEGPAYRALI
                     AALVEHFVDGSSRGALGHSPASTITERATLLGRGVPVPESVAELARASGEAGGAAKAR
                     RTVAQAMATVMAGQGFRTEELPRGRGLRLVNCPLVGVAVRHGEVVCGFHQGMLQAVVE
                     RSGGDPDSVHLEPFAEPGACLVRIGPATSS"
     gene            9827..>10686
                     /locus_tag="PLK64_06745"
     CDS             9827..>10686
                     /locus_tag="PLK64_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HPV79476.1"
                     /translation="MSINLIRPKPSSGGGLDVSDPASDLVSDPASGPISGLVDRGPVD
                     HGPGHRIVGLLTTTDHKVIGNLYLVTSFLFFMLGGLLALGIRAELAAPGLQIVDNPEQ
                     FNQLFTMHGTIMLLLFATPLFAGFANAIVPLQIGAPDVSFPRVNALAYWFYLFGGLVV
                     ISGFLTPGGAAAFGWFAYAPLSTGAYSPGLGGDLWVFGLALTGFGTILGGVNFITTIV
                     TMRAPGMTMFRMPLFTWTVLVTSVMVIVVFPVLAAALFGLGADRRFGAHIFDVEAGGA
                     MLWQHLFWFFG"
BASE COUNT         1489 a         3717 c         3768 g         1712 t
ORIGIN      
        1 cccggcgagc cagcccggcg gcgagccggg agacgtcgtg gcggagggcg gcatacgtcc
       61 acgcgaccgg aggaccgtcg aaggggaccc aggtgagcgc cggggtggac ggcgtggctg
      121 ctgcccaata gccgagcaac ccgccgaggt cgccgcttcc gtcggctgcg gcggtcatgc
      181 cccgccgaca gtgcccgacg cttcgcccaa gggccgcgcc gaccagccgt caccgacgtc
      241 catcgccagc gccaacgact gaaaagactt gccgtgcggg tcgattcgca acgtcgccga
      301 gaccccgccg tcgagggcct ggaacagcac gaagttgagg ccctgcactc gcggcaactc
      361 atagcggacg acctcgccgc gcaccaaccc cgcgaagtgt tgcctgaccc gctcggcggt
      421 gagccgctcg acgagcatcg gatagtcggc cggctcgtgg acgaacacgc agatgttgct
      481 cgtgtcgccc ttgtcgccgg agcgggcata ggccacttcg cggaggatca cgagcgcacc
      541 acctcggttg tgagagtgac caattcgcga ggcacgaagg cgggcgtgac gccgagcgca
      601 gggaccaccg aggtgacgac cccgccgccg cccgatggcc cgaagtagag caactccacc
      661 tcgtgcgcgg ccgcatcggc gacggcccgg gagggggtgt gaaccgccaa acgcagtcgc
      721 acctcggccg gctcggggct cgtgggtcgg tctccgaaga gcgaagtgag ccccacgagg
      781 tcgacgcgga cgtcggtgag cgacgcggca tacggctgca ggtaggcgcg caggacctcc
      841 tcggcgcgcc gcgcgcgggt cacgcacccg ggcccgccga acgagatctc gcccgtcgcg
      901 cggaagccgt cgtcgacgcc gacgagcacc ttgagggtct cgggccgtgg acgtccgcgg
      961 gctccccata gccgcactcg gtcgccgccg aggtcgtcga tctcgatgtc ggagaagtcg
     1021 gcggtcacgt ccggcgtcag gtatgccgcg gggtcgtgga tctcgtaata cagctgggtc
     1081 ttcatcgtgc gggcatcgac cgcaccaccc gtgccgggga gtttggtgat gatcgcgtcg
     1141 gcccgagtga cgtcggccag cgggaagccg agccgatgcg ggtcggggac gacgcggtag
     1201 ggcgggtcct cgaagttgcc cccagtgctg tgcactccac actccagcag gtggccgacc
     1261 atcgtcgcgt gcgcgacggc gtcccagtcg tccagcgccc acccgagctc gtggcagatc
     1321 ggcccgacga acagcgacgg gtcggcgatc cgcccgccca cgacgatctg cgcgccggcc
     1381 tccaggcagg tgacgatcgg gtcggcgccg atgtaggcgt tggccgagac gacccggtca
     1441 cccagggcgg cgaccgtcgt gccccgttct gggagagcgc agtccaagga ccggacgagg
     1501 tcaaggacgg cgtcgccgcg gatgacccca atgggtatgc cgtgctgccc ctgggcgcgt
     1561 agcgagtcga cgaggtcggc cacggccgcg tcggggttgg cggcgccgaa gttgccgacg
     1621 acccggtggc cgcgtccgag gtgacccgcg agcagcggga cgagttcgcg gatgcgttcg
     1681 tcctggccgg tcgccggatc gcgccgccgg cgctgatggg ccagcgccat cgtgcgctcg
     1741 gcgagcgagt cgaacccgat gtaggtgacc cgccccgagt cggccatcgc ggccgccggg
     1801 gcgagtcggt cgttggcgta ggccgaaccg gcgccgatcg acaccccaac ctggatggcg
     1861 cctcgaggca ttgcgctcag tccgagacgt cgatggcgag ctcggggcag ctctcggcgg
     1921 ccaggagcac ggcctcgcgc tcctcgtcag gcacatcctc cgagcggact tcggcgtggc
     1981 cgtcctcgac ccgcgcgaag aagaagttcg gcgcgctcag ccggcacagg ccgtgaccct
     2041 ggcagcgctc ctcgttgacc tggaccttca tgttgccctc cacgtgtcgt tgacaggacc
     2101 attcagtata accataatca cattaaggcg tggagggaag agagccacgc gaacggccca
     2161 tcagcagcct agggaggccc aaccatgacc gagacccagc ggtgcccagt cgtccatttc
     2221 gaccacaact ccgacgaaca ctcggccgac ccggcgggct cctacaagcg catccgggac
     2281 gagcaccccg ctgcgtggag cgaggctcac ggcggctatt gggtgctctc cggctaccgc
     2341 acggtgttcg acgcctcccg agacgacgcg acgttctcgt cggcgcgcaa cgagcacggc
     2401 ggtgaaggtc tctcggtggt catccccaag acccccatgc acttccacat ccccatcgag
     2461 gtcgacccgc ccgacttccg caagtggcgc aaactcatca acccgatcac cgctcccgct
     2521 gccgtcgagc ggatggagac gatggtccgc cactacgtca cgtggttcat cgacgagatc
     2581 atcgagaagg gctcgtgcga catggccgag gtcatcggcg tgccctcgat cgtcaccgtc
     2641 gactggatcg gtctggaccc cgccgactgg aagcgctacg ccttcgccca ccgcatggtc
     2701 ctcgccgcgg tgccgggcag cgacgactgg aagcaggcca tcgaggtcga gttcccctac
     2761 atcaacgccc gcacgtggga ggccatcgcc gaccgccgga tcaaccccac cgacgacatg
     2821 atcagctact tcgtccagca ggagatcgac gggcgtccga tcaccgacga cgaggtcttc
     2881 tcgatcgtcg acctgctgct ggccggtggc gtcgccacga ccgcgtcgct cgtctccaac
     2941 acggtggtct ggctctacca gcaccaggat gtgcgccagc aactcatcga cgacccgagc
     3001 ctgttggaca aggcgatcga ggagttcctg cggttcttct ccccgaccca ggcgttggcc
     3061 cgcaccgtca cccaggacac cgagttccac ggatgcccga tgcgcgaagg tgaccgggtg
     3121 ctcctgtcat gggcctcggc caaccgcgac cccgagcagt tcgaggaccc cgacgacgtc
     3181 aacatcgagc gctggcccaa ccggcatacc gcgttcggga tcggcgtgca ccgttgtgcc
     3241 ggctcgaacc tcggccggtt catggccaag gagatgctgt cccagatcct cacccggatg
     3301 ggcgactacg tcatcgacct cgactccctg caggcctacc cgcaccaggg cacgaacacc
     3361 ggatggcagc ggatcccggc cacctacacg cccggcccgc gcatccttcc tgttccggcc
     3421 ggctgagccc ggctaagccc ggatcgcccc cgtcccccga cccatcccca cgtcaccacc
     3481 gcagaggctg atcaccccat gaatccgcac gtggtcgtcg tgggtgccgg tctggccggc
     3541 ctacgggcgg ccgagcagtt gcgcgccgca ggtcacgcgg ggtcgatcga ggtcatcggc
     3601 ggcgagccgc acctgcccta caaccggccg ccgctgacca aacaggccct cgtcgccggc
     3661 cccgatgtga tgaccttgat gttcccgcgt cgaccgtcga tcgagaacgt tcgctggacc
     3721 ctcggtcggc gcgcggtctc ggtcgacctc gacgggtcga cgctcagcct cgacgatggg
     3781 tccacccgtg acttcgacgg gctcgtcatc gccacgggtg tccgctcgcg gcgactgccg
     3841 attggcgagg gcggcggccc tgctccgaca ctgaccctgc ggactgtcga cgatgcgctc
     3901 gccctgcacg ctcggctcgc cccgggcgcg cgggtggtcg tcatcggggc cggcttcatc
     3961 ggctgcgagg tggctgcgac agtggcggct cgcgggggcg cggtccacgt cctcgacccc
     4021 ctgccggcac cgttggcagg cgtccttggg cccgcggtgg gctccgcagt ccgcgcgcgg
     4081 ctggaagccg cgggggtggc cttcagcctc ggcgtcggcg tcgcccaggt gctcgccacc
     4141 cccaccggtg ggtatgccgt gcgcctcacc gacagcgcgg acgtcatcac ggccgacgtc
     4201 gtcgtcgagg ccgtcggctc ggtgcccgaa accgagtggc tcgccgggca gggcctggac
     4261 ctgggcaacg gaatcggctg cgacggcgac ctgttggtga cgcgaggcgg catacccctg
     4321 aggaacgtcg tcgcgctcgg tgacatcgcg cgcttccccc acccgtggtt cggtcaggag
     4381 atccgggtcg aacactgggc ctggcccacc gacctggccc ggcgagccgc cgccagcctg
     4441 gtcgccgggc tcgggccgac gcccgccccg gacaccccgt tcaccccgct gccaagtttc
     4501 tggagcgacc tgcccggtgg gcggatccag tccttcggtc tgccgccgct cggtctgggc
     4561 gacgtgcgat tgctcgaagg tgagtgggag ggcgactgcg ccgtgggcta ccaccgcgac
     4621 ggacgtctgg tcgggttggc cctggtcggg ctggcctcgc ggatgatcga ctaccgcacc
     4681 gccctgctcg acgacctgag caccggcacg ggcgctggcg ccgacgccgg gccgaccggg
     4741 tgaaccgcgc cgggccgcga gcggaccccg atccggctcc cggcggctcg gcgcgacgtg
     4801 cctgggcgtt ggcggcgttg gtcaccctct tcgggctcaa tctgcgggcc acgctgggat
     4861 cgatcccccc gctgctcggt gagatcgcca cggacctcgg gctgggttcc agcgctttgg
     4921 gattgctgct gtccacctgc atcctcgcca tgggcgcctt cgcgccgacc ggccatctcc
     4981 tggccacccg ggtcggcacc gaacgggcgc tgaccctcct gctggcgatc ctcggggtgt
     5041 ccgaggtggt gcgcgtcgcg gcataccacc cggtcgtgct ctatgcctcg gtggcgctgg
     5101 cgggcgcgac catgggcgcg gcctccaccc tcatgcccgg tttcatcgcc caccacgtgc
     5161 cacggacccg cgggttgacg atggggatct actcgacggg gctggcgctc ggggtcgcgc
     5221 tggcggctgc gaccgctgtg ccgctcgagc ggctcctcgg gggctggcgt ccggcgctcg
     5281 ccgtggtcgg cctcctgtcg ctggccacgg cggtcctctc gtgggcgtcg gaggggacgc
     5341 tggtgcgaag catgcctggg cacagcgtcg acccgccgcg cacccgtgat ccgctgccct
     5401 ggcgcagtgt tccggctcgc tggctcaccc tgctcaacgc actcgtgatg gccattggct
     5461 tcagcggatt ggcttgggtg gcaccgtatt acgtcgacct cgggatgtcg gtccaggacg
     5521 cggccggcat ggtggtgctc tttcaggtcg tccaactcgt ggcgatgctg gctctgccgg
     5581 gcatcaccga tgtcacgacc gaccgtcggc cgttggtggg cggaatgttg ctggcgaccc
     5641 tcgccggcac ggtcatgctc ggggtggcgc cgatggctca gccgctgctc gcagtcgggc
     5701 tcttcgggct gggggcgggc ggcatggcca cgttgctgtt ggtgttgagc gtcgacctca
     5761 ccgattcgcg gtcggcgtcg gcgtccctca atgcgatgac gatgctcgtc ggttatgccg
     5821 tcggcgcggc cgggccgttc ctgctcgggg cggcgcggga cgtgaccggc agcctggggc
     5881 cgggctatct cgtcgtgtca gggctggtcg tcgtcagcct ggtgatcgtc tcggtcttcc
     5941 ggcccggcct caaggttgcg cgggccgact agcggcgcgt cagccgcggg aggtgtagta
     6001 gggcgcgagc caggcgttgc cggtctcggc gtagagcacc tcgggccaat ctgagggccg
     6061 gtggtcgcca taggcgcgga cgctgttgcg gtcgcccagg ccttcggaca actcggtgaa
     6121 cggcaggtag tacatgtagg acatcagccg gtcagcgaag cgccaacggc cgtcgacccg
     6181 gcggtaagtg tccttgtaac gcaacgcgac ctgcatcgcg acgccgttgc gcgagacctc
     6241 ggcgtggctg gcgatcagcc ccgtggcgcg gtcggggtcc gtgtcgtcga ggatgacgac
     6301 gtgcccgtgg ctgaagtggt tggtcgggcc gagcgcggcg aaccggccga ggtaggtctc
     6361 gacgatctcc tctatcccgt tggccttgaa gaccccgtcc tgggagcgca gggtcgcgtc
     6421 ctcgcagaag atctcacgga tgccgtcccc gtcgcgttcg tccatgacga acccgtagag
     6481 gatgcccaac tcctggatcg cggcccggtc ttccaggcga cggatccgct cgtcgaggct
     6541 gcgttcggcc atgaggactc ctttgaactc ggcagtgcag ggggtcaaaa gcagtgctgc
     6601 ggtcaggaga ggtaggcgcg caactcgggc ccgaggccca gcacgaccga atgctcaccg
     6661 tgggtgagtc gcagaccgcg gtcgtgccac ttggccttcc aaccgtgcaa ctcgaggccg
     6721 tcggtcagcg tggccagcaa ggcgtcccgc cgttcggctc cgtcgctcgt ctcgtggttg
     6781 acgtgcacga ggacccggtc ggcgagggcc tgggcatcga gctgggccag ttggctgatc
     6841 cgctcgtcga cgtcgagcag gaccggcgcc atgaacagat cagcccgatc gtgggggcgc
     6901 ttcttcggca tgcggtgctc cttcatgcgg tcgtcacggc gcatcgaagc gcagattcag
     6961 tataaccatg attaccatag ggctggaaga aggagggcgc cagatgcctg acgacgtgct
     7021 cgctcacccc gagtcttcgg gttcggctac cgacgtggat gtcctcgttc tcgggacggg
     7081 ggccggcggc ctcaccgccg cgttggcggc cgccacggcg ggcgcgcggg tcgggctcta
     7141 cgaaaagggc gagcgagtcg gcggcacgac ggccctctcc agcgcggtcg cctggctccc
     7201 cgccaaccgc tatgccgcgc aggcgggcat cgcggactcc cgcgaggacg ccctggccta
     7261 tctgtcggcc ctctcccacg ggctcattcg accggagttg gccgaggcct tcgtcgacac
     7321 ggtgccggag ttgcttgact ggctcgacga gcagaccccg ctgcggatgc ggctcgtggc
     7381 cggcttcccc gactatcacc ccgagcaccc cgggggtatg ccgcgtggcg gccgatcact
     7441 cgagccggag ttgttcccct tcaccgagat tccggggtgg ggtgagcgga tggtcggtgt
     7501 gacccgaccg atgctcgtcg ccgagactcc gatcgggggc gggtcggggt tgcttcccgc
     7561 cgatgtcgcg gcgcagcgca aggccgcctc gctggagggc gtcgggcgcg ggctggtcgg
     7621 cgccctgctc aagggttgtc tcgatcgtgg ggttgagccg gttaccggag cgcgggcgac
     7681 ccgactggtg gtgaccgacg gcgccgtgac cggcgtcgag ttcgactccg gtgcagagcg
     7741 attcgtcgtc actgctcgct ccgtgatcct cgcgaccggc ggcttcgagc acgacctcga
     7801 cctggtccgc agcttcctgc gcggtcccat gcaccttccg gcaggcgtgc cgaccaacac
     7861 cggtgacggg ctgcggatgg ccatgcgggt cggcgccgag ttggggatga tgagtgaggc
     7921 gtggtgggtc cccgtgctcg cgctcccgca ggccgacccg gccgcgaccc accggccggt
     7981 gctgctggtc aacctcgagc gcaccctgcc gcgttcgatc atggtcaacg gtcagggccg
     8041 gcgcttcacc aacgaggcgg ccaactacaa cgccctcggc ggggccttcc accacctcga
     8101 tcccacgagc ttcgcctacg tcaaccagcc ggcttggttg atcatcgacc aaggtttcgt
     8161 cgaccggttc ggcggcttcg gcgtgcctcc gggaggtccg gcgcagccgt ggctcatccg
     8221 cgccgacgac ctcggcgcgc tcgccgaggc gaccggcata cctccaactg ccttgtccga
     8281 cacggtggtt cggtggaatg agcaggcggc ggcgctgcgc gacgacgact tccaccgcgg
     8341 ccagagcgcc tatgacgggt ggagtggcga acggcggcac tacccggggc cggccgccac
     8401 gttggggccg ctcgacgaac cgccgttcta cgcggtggag atcgtcggct cgtgcctggg
     8461 gaccaagggc ggcccgcgca cggacgtgga cggccgcgtc caacacctcg acggtggccc
     8521 aatccccggt ctgtatgccg cgggcaacgc catggcggcg ccgaccggca tggtctacgg
     8581 cggggcgggc ggaaccctcg gcccggcgct ggtcttcggc taccgtgcgg gaaggtcggc
     8641 agccagggag tgctgaccgc gccaggcctc gggatcctcc ggtcagcgca cccggtgcag
     8701 gcgcaggcgc cacgcctcgg gaccgcgctg taggtagctc acctcgacag cgtcccccga
     8761 acgggcgctg atctgggcca gcagcggcag cgggtcgtgc ggggcgacga ggaccatcgc
     8821 gtgccccggc cgcaccgagt cgagcgcgcc gaagatcgcg gcgtgccgaa tggcgtgcgg
     8881 gatggtgcgg gcgtcgagtt cgggcaccgc ctcctcgtgc tcagcgcagg cgcagccgcc
     8941 cgtctcgtgg acgtcgacga cgggcaactc ggcaggctgg tgcgacatgc gtatttctcc
     9001 tcaggtgggg acggacgggt ggtggatcgg ctcaggacga ggtcgctggg ccgatccgga
     9061 cgaggcacgc gccgggttcg gcgaacggct cgaggtgcac tgagtcgggg tcgcccccag
     9121 atcgttcgac gacggcctgc agcatcccct ggtggaagcc gcagacaacc tcgccatgtc
     9181 gaaccgcgac cccgaccagc gggcaattga ccagacgcag ccctcggccg cgcggaagtt
     9241 cttcggtgcg aaagccctgt ccagccatca ctgtggccat cgcttgggcc accgtccggc
     9301 gggccttggc ggcgcccccg gcctcgccgc tcgccctcgc aagctcggcc accgactcgg
     9361 ggacgggaac gccacgccct agcagggtgg cgcgttcggt gatcgtcgac gcgggtgagt
     9421 gcccgagtgc gccgcgactt gatccgtcga cgaagtgctc aaccagcgcg gcgatgagcg
     9481 cgcggtaggc ggggccttcc gaggggtgcg gcagggcggc ataccggtat gccggtcgcc
     9541 cccgccccgc gggtgcgtcg cggtcgcgcg ccgccagccc gaggtcgacc aaggcatcga
     9601 ggtgttcgcg caccgtgttg gggtgctgtc ccgtccgcgc gcccaactcg gcggcggtcg
     9661 cgggcccgtc ggcgctcagc gcggcaagga cctccccctg ggcgggggta gcgacggccg
     9721 tcggttcctg cgtcggcgcc ggcccgagaa cgggcgatcg gtacggggta tttaacacgg
     9781 ctctaagcgt actatccaag aacagtccag ggaaacgggg atcgccatgt cgatcaacct
     9841 cattcgcccg aaaccgagtt cggggggcgg cctcgacgtc tcggacccgg cctccgacct
     9901 ggtttccgac ccggcctcgg gcccgatctc gggccttgtc gatcgcggtc cggttgatca
     9961 cggtccgggg catcgcatcg tcggcctgct gacgaccacc gatcacaagg tcatcggcaa
    10021 cctctacctg gtgacgtcgt tcctcttctt catgctcggc gggttgctcg cgttggggat
    10081 ccgtgcggag ttggccgcgc cggggctgca gatcgtcgac aaccctgagc agttcaacca
    10141 actgttcacg atgcacggga cgatcatgtt gttgctcttc gcgaccccgc tcttcgcggg
    10201 gttcgccaac gcgatcgtgc cgctgcagat cggtgcgccg gacgtctcct tcccgcgcgt
    10261 caacgccttg gcctactggt tctatctgtt cggcgggctc gtcgtcatca gtggcttcct
    10321 cacccccggt ggggcggcgg cgttcggatg gtttgcctac gcgccgctgt cgacgggcgc
    10381 ttacagtccg gggctgggcg gtgacctgtg ggtgttcggc ctggcgctga ccgggttcgg
    10441 caccatcctc ggcggggtca acttcatcac gacgatcgtc accatgcggg cgcctgggat
    10501 gacgatgttc cggatgccgc tgttcacctg gacggtgctt gtcacgtcgg tcatggtgat
    10561 cgtcgtcttc cccgtgctgg cggcggcgct gttcgggctg ggggctgatc ggcgtttcgg
    10621 cgcgcacatc ttcgatgtcg aggccggcgg ggcgatgctc tggcaacacc tgttctggtt
    10681 cttcgg
//
LOCUS       DAOMZY010000089        10630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5833_length_10630_cov_5.531820, whole genome shotgun sequence.
ACCESSION   DAOMZY010000089 DAOMZY010000000
VERSION     DAOMZY010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 10630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10630
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5833_length_10630_cov_5.531820"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="PLK64_06750"
     CDS             complement(<1..1073)
                     /locus_tag="PLK64_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79477.1"
                     /translation="MHRTLRHDLILGEETAEGRVTVTDPVANRHYLMTGATVRVLRMF
                     DGSRDDNQVYAALAGIGVHVSDQTFSAMAQRAEQLGLFQGGQPPPVPSHLRGPQRGNL
                     LFLLLGEFDPHRVVDALAPVGRVLFSRMAAGTLLALLAWCVWLVVQHSDRYVADMTAF
                     AMVSAWALVYLANAVVTVMHEFGHALATRRFGGQVHRMGLALYLLSPAAYTDTSAAWA
                     FSKNRHRIVVNLAGIYVESFVLAGCVLLWVFDVLPVTFAAANFVLLHALLARIIFNLN
                     PFLRLDGYWLLSDLMHIPNLRPRALRTLLALLSGHRRSAPAVASTEERAFLVVYGILS
                     VAFAVGGLVVGALVIDGLARRFVP"
     gene            complement(1106..2422)
                     /locus_tag="PLK64_06755"
     CDS             complement(1106..2422)
                     /locus_tag="PLK64_06755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HPV79478.1"
                     /translation="MTTDLAFERTLHLANQLISPVTGVLKRSLLLPLVGGQAPFAHFA
                     AAPASYGTLPTGGDIANPGASAWDLRQCLSQVTFEAVERYCAAFVDYSALLLSKPEGP
                     AFDVGCAIQRFAEFQYGRSDFSYVPLRQDSVIHWAVGRSLFTGQRRYIPASFTYLPYR
                     PTSPDEVIGPSFSTGMSAAWTLDDACLSGLLEVVERDAFAITWMGQLVRPRLEPRPDS
                     ELARRVAAVEADGCSSVTFVDLTTDLGLPVVCAVVRRPALGRVLTTVGLSSKLCPSRA
                     CDKALCEALSDHERLRTELADPRRPPWTPAPDFSDVVDFEWHGRAYTDPRAQLALDFL
                     TSSSEVHQIDGEFAPPQAGDLVRTLRQLEEHCSDVVMVDLTTRDMHELGLVVVKIFVP
                     ELVPLNADHRYPYLGHRRLHTHGGQRPDGTQEQLLTHLNSYPHPFS"
     gene            complement(2419..3282)
                     /locus_tag="PLK64_06760"
     CDS             complement(2419..3282)
                     /locus_tag="PLK64_06760"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HPV79479.1"
                     /translation="MPEPLNKQSYSLVDFFEEDDYSESETYNEYTKLTRMNVARISHR
                     VEQIKSDPAMLAMMARSWKSYPGSTVTPLPPFRAPTVGFAETVLRRRSVSSLGRDFVG
                     EAITAEQLGGTLVMAYGPTSKIESRTGDVQHLRATTSAGALFPTEIYVLAFNVTGIEP
                     GLYHYRPIEHSLELIRSGDLRAQFAGLSSYQGLCLTSSVALVLTSVLKRTMSKYQHRG
                     YRFAMYDCGALTQSLYLSGTAMGLDTCALGGIYDDEVAAFLGVNPVDEPVQLGFLLGP
                     RGAEMGTEKQQ"
     gene            complement(3322..4629)
                     /locus_tag="PLK64_06765"
     CDS             complement(3322..4629)
                     /locus_tag="PLK64_06765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HPV79480.1"
                     /translation="MGVESRQDLGGALDWMVGPTSIVRAVRTILNAPGEPRLFSVASV
                     PNSLTSIAGAEMAGHTGAMAETWEQAAASAIGECVERYCCAVQPPNLLTAPARQLGEG
                     ALLVDDFELFDERQYAHPQFPFARHTEDLPMTWVPAARIRDGEPRFVPACLVYIPYVP
                     RLVEHSDMVALSVSSGQACHSDADLALLSGLYEVIERDAFMLTWVRRLPPTRLNYRDD
                     PVVGPWFDRYFADSSLTFDVFRLRSDIAVPSVLCVARGIHHQGPFACVGASCRLDESE
                     AVRKAIVEAAQGAVWVRDLIDTKPHWEHGPDYANVRDFEDHVRLYGDPGMLEHLDFLY
                     VDEWDGLAPAPDVTGLRAQREQVLDCLEAVGLQALVVDITTRDVADAGLVVLRVLIPG
                     SVQLYSVHGLPNFGARRYHTVPPRLGFDADIHRSYNPVPHPFP"
     gene            complement(4634..5782)
                     /locus_tag="PLK64_06770"
     CDS             complement(4634..5782)
                     /locus_tag="PLK64_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM precursor leader peptide-binding protein"
                     /protein_id="HPV79481.1"
                     /translation="MTASAPQRGNLRLGEVYLVPAADGGLTMRTPRRSAVLRGRLVSE
                     VFPRLLPLLDGTRDLEGVLTELASDGEFPNLEASLRQLLDMGVVEFAEPAAELEGVPS
                     QLLPKLRGQDRFMRQHGGDDGTTARIASGSVLLAGEGPLMPDVAVGLAASGVRRIVVA
                     ARRTVGAVDVGQSTHIGPTDLDRSMGEVVAAVLKRNDLPTLVEPAPFPESKLGWQQLL
                     GDIDVAVVVQDAPVVSLPWMDEFNRATIAAGTPWTTGALLQRANVHVGPSVIPGQTAC
                     WKCFEFRFKSNLSSVVRYEEFQDYVASLERYVEHGSLPSVTAFTGAMVALEGVRLLAA
                     GDVFVRTAGSLLAFDLWELRLETHPVLKLPRCPHCSTVSSVPQERTWS"
     gene            complement(6061..6204)
                     /locus_tag="PLK64_06775"
     CDS             complement(6061..6204)
                     /locus_tag="PLK64_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79482.1"
                     /translation="MNRSTEVELVDEMFSIEELEQRETPGSWSCGCSCSCSCTSCTCV
                     YTW"
     gene            complement(6433..7032)
                     /locus_tag="PLK64_06780"
     CDS             complement(6433..7032)
                     /locus_tag="PLK64_06780"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPV79483.1"
                     /translation="MTEAERTMRRRRIVVRIVLFVTIIGLGLAVVSGTLGMKPGLLTH
                     TDDPIMVGGSGPGGEVNSVIPSISGVNYANQPVSVPTGHPVVLLVVAHWCEYCKADLA
                     LMSQGHAAGTFRPKADIVIVSTRHLPFLSWPPSEALSLDSVPFPVIVDTDSSIATYLA
                     LRVTPTWYFIDRTGTIRKVVAGTLSLAEIDDEINAVTRE"
     gene            complement(7566..8156)
                     /locus_tag="PLK64_06785"
     CDS             complement(7566..8156)
                     /locus_tag="PLK64_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HPV79484.1"
                     /translation="MTDPADATPGQRAVAALAAYVADAELTSEPGARDGEVVVTIPGE
                     RKLKTVASFLVRDELTSVSAFVIRNPDENHAEFYRFLLRRVMRRPLLGYAVDGSGDVY
                     VRGQVPTVALDGRYLDLLVGALLEAADEPFNDLLAIGFLTSMKREWAWRVARGESLAN
                     LEAFRSILAGSEDDPLYAVGPLPVDPATPDDRADSR"
     gene            complement(8235..9275)
                     /locus_tag="PLK64_06790"
     CDS             complement(8235..9275)
                     /locus_tag="PLK64_06790"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPV79485.1"
                     /translation="MRIAVAGAGLIGRRHIEELVKSPHASLASIIDPSPEAGALAAEL
                     GVPHYVTLERALADDRPDGVILATPNRLHVEGGLTCIAAGVPVIVEKPLAETAAGAKQ
                     LVEAAEAAHVPLLTGHHRNYSANMAAAREVISSGVLGRLVAIVGQATFYKPDDYFDVG
                     DGWRRKAGGGPILLNLTHEVNNLMALAGDITAVQATTSSATRGFEVEDTAAMIFHFAG
                     GVLGTFMLSDTAASPRSWEQTSGENTSYATYPDEDSYHVMGTVGSLSIPTMRLKTYPG
                     VQSWWSATDDSRIDVAVEDPLANQIAHFAAVIRGEAEPICDGRAGLKTIQVCDAVQEA
                     ARTGAVVNTGIS"
     gene            complement(9404..>10630)
                     /gene="mshA"
                     /locus_tag="PLK64_06795"
     CDS             complement(9404..>10630)
                     /gene="mshA"
                     /locus_tag="PLK64_06795"
                     /EC_number="2.4.1.250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-inositol-3-phosphate glycosyltransferase"
                     /protein_id="HPV79486.1"
                     /translation="GGMNVYIAEVAKELARQNREVEIFTRATTGALPPTVELVPGVTV
                     RHITAGPYEGLAKNDLPGQLCAFAAGVMRAGAARPEGWYDLVHSHYWLSGQVGWLAAD
                     RWRVPLVHTMHTMARVKNAALADGERPEPAGREIGEAQVVEAADLLTANTADEACQLV
                     SLYDADPARVVVVPPGVDLEVFTPGDRYAARSRLGLPQGAHVVLFVGRIQPLKAPDLL
                     VKAARRLLDAHPDWTPDDLVVGILGGPSGTGLEHPESLATLAAELGMTAYVRFVPPAD
                     RATLAQWYRASDLVTVPSYNESFGLVAIESLACGTPVVAARVGGLPTAVGPGGVLVDG
                     HDVDDWATTLDRLLADPGLRAELGRRAVRHARSFGWGATTERLLQAYGEACRTRTPSP
                     IFEASLLTGVPAAVVP"
BASE COUNT         1951 a         3447 c         3571 g         1661 t
ORIGIN      
        1 ggcacaaaac gccgggccag accgtcgatg acgagcgcac cgacgaccag accgccaacc
       61 gcgaaggcca ccgagagaat cccgtagacc accaggaagg ctcgctcctc ggtggaggcg
      121 accgctggag ccgaccgccg atgacccgag agcagggcga gcagcgtgcg cagcgcccga
      181 ggccgcagat tggggatgtg catcaggtcg ctcaatagcc aatagccgtc caaacggagg
      241 aacggattga ggttgaagat gatccgtgcc agcagtgcgt gcagcagcac gaagttcgct
      301 gcggcgaagg tcacgggcag gacgtcgaac acccagagca gcacgcatcc ggcaagcacg
      361 aacgactcga cgtagatccc cgcgaggttg acgacgatcc ggtgccggtt cttgctgaac
      421 gcccaggccg cggacgtgtc cgtgtatgcc gccggggaaa gcaggtagag ggcaagcccc
      481 attcggtgga cctgtccgcc gaatcgcctg gtggcgagcg cgtgtccaaa ctcgtgcatc
      541 acggtcacga ccgcgttcgc caggtagacc agcgcccagg cagagaccat cgcgaaggcg
      601 gtcatgtccg ccacgtagcg gtcactgtgc tgcacgacca accacacgca ccacgcgagc
      661 agggccaaga gcgtccccgc ggccattcgg gagaacagca cccgcccgac gggagcaagt
      721 gcgtcgacga cgcggtgcgg gtcgaattcc cccagcagga ggaacaggag gttgcctcgc
      781 tggggaccgc gaaggtgcga agggactggc ggtggctgac cgccctggaa cagtcccagt
      841 tgctcagccc gttgagccat ggccgagaag gtctgatcgc tcacgtggac cccgatcccg
      901 gccagcgcgg catacacctg gttgtcatca cgcgaaccgt cgaacatccg cagcacgcgc
      961 accgtcgcgc cagtcatgag gtagtgcctg ttggccaccg gatcggtcac cgtgacccgc
     1021 ccctcggcgg tttcctcgcc gaggatcagg tcgtggcgca gcgtccggtg cacggtatcg
     1081 gcctccttga cggatcgtgt cgcgttcacg agaaggggtg ggggtaggag ttcaggtggg
     1141 tcaacagctg ttcttgcgtc ccgtctggac gctggccgcc gtgggtgtgc agccgacggt
     1201 gaccgaggta ggggtacctg tgatccgcgt tcaggggcac caactccggg acgaagatct
     1261 tgacgacgac caggcccagc tcgtgcatgt cgcgggttgt gaggtcgacc atcacgacgt
     1321 ccgagcagtg ttcttcgagt tgccgcaggg tccggaccaa gtcccccgct tggggcggcg
     1381 cgaactcccc gtcgatctgg tgcacctcgc tacttgaagt gaggaagtcg agggctagct
     1441 gcgcgcgtgg gtcggtgtat gccctgccgt gccactcgaa atccacgacg tcggagaagt
     1501 caggggccgg cgtccacggg ggccgccgag ggtccgcgag ttcggtgcgc agccgctcat
     1561 gatccgagag cgcctcacag agcgccttgt cgcacgcccg tgacggacag agtttggagg
     1621 acaggcccac ggtggtcagc actcggccga gcgctgggcg gcgcacgact gcgcacacca
     1681 ccggcaagcc gaggtcggtc gtgagatcaa cgaatgtcac gctcgaacat ccatccgcct
     1741 cgacggcggc gacccgacgg gccaattcgc tgtccgggcg cggctccagc cgcggacgga
     1801 ccaactgacc catccaggtg atggcgaagg catctctctc gacgacttct aggagcccgc
     1861 tcaagcaggc atcgtccagc gtccaagcgg ccgacatccc ggtcgagaac gagggaccga
     1921 tgacttcgtc cggcgaggtg ggtcgatagg gcaggtatgt gaaggaggcc gggatgtacc
     1981 gccgttgacc agtgaacagg gatcgcccca ccgcccagtg gatgacggag tcctgacgaa
     2041 gcgggacata actgaagtcg cttcgcccgt attggaactc tgcgaaccgt tggatcgcgc
     2101 agccgacgtc gaaggcgggg ccttccggtt tggacaagag aagggcggag tagtcgacga
     2161 acgcggcgca gtagcgctcc accgcttcga acgtcacttg cgagagacat tggcgcaggt
     2221 cccaggctga ggcccccgga ttggctatgt caccgccagt ggggagggtc ccgtagctcg
     2281 caggcgcggc agcaaagtgc gcaaacggcg cctggccacc gaccagcggc agcagcaacg
     2341 accgcttcaa gaccccagtg accggcgaga tgagctgatt ggccaggtgc agggtgcgct
     2401 cgaacgccag atcggtcgtc actgctgctt ctccgtcccc atctcagcgc ctcgggggcc
     2461 caggaggaaa cccaactgca ccggctcgtc gacggggttg acgccgagga acgcggcgac
     2521 ctcgtcgtcg tagatgcccc ccaacgcgca ggtgtccagg cccatcgccg tgccggacag
     2581 gtacagcgac tgggtcaggg caccgcagtc gtacatggcg aagcggtatc cgcggtgctg
     2641 gtacttggac atggttcgct tcagaacact ggtcagcacg agggcgactg aactggtcag
     2701 gcagaggccc tgataggagg acaacccagc gaactgggcc cgcaggtccc ccgaccggat
     2761 gagttcaagg gaatgctcga tcgggcgata gtggtagagg ccaggctcaa ttcccgtcac
     2821 gttgaaggcc aggacgtaga tttcggtcgg gaagagcgcc ccggccgagg tggtggcccg
     2881 gaggtgttgc acgtcacctg ttcgagactc gatcttggac gttggcccgt aggccatgac
     2941 gagggttccc ccgagttgct cggcggtgat cgcctccccg acgaagtcac gccccagcga
     3001 actcaccgac cggcgacgca gcacggtctc ggcgaagccg accgtcggag cgcggaaggg
     3061 cggaagtggc gtgactgtcg agcccgggta ggacttccag cttcgagcca tcatcgcgag
     3121 catcgcgggg tccgacttga tctgctccac ccggtggctg atccgggcga cattcatccg
     3181 ggtcaacttg gtgtactcgt tgtaggtctc gctctcggag tagtcgtcct cttcgaagaa
     3241 gtccacgagg gagtagctct gcttgttcag cggctcgggc atcgggctct ccttggtgct
     3301 gacgcggaac gcgctcggtg atcagggaaa cggatgaggg actgggttgt aacttcggtg
     3361 gatgtcagcg tcgaacccaa gacggggtgg gactgtgtgg tagcggcggg cgccgaagtt
     3421 cggcagcccg tgcacggaat acagctggac acttcccggg atcaggacac gcagcaccac
     3481 cagccccgcg tctgcgacgt cgcgggtcgt gatgtcgacg accaaggcct gcaggccaac
     3541 cgcctcaagg cagtccagaa cctgctcccg ctgggcgcga agcccagtga cgtccggcgc
     3601 gggcgcgagg ccgtcccact cgtcgacgta aaggaagtca aggtgttcaa gcatgcccgg
     3661 gtcgccatag aggcgcacgt ggtcctcgaa gtcccggaca tttgcgtagt cggggccgtg
     3721 ttcccagtgg ggcttggtgt cgatgaggtc gcgcacccag acggccccct gggcagcctc
     3781 gacgatggcc ttgcggaccg cttccgactc atcgagccga caggacgccc ccacacaggc
     3841 gaacgggcct tgatgatgga tgccacgcgc gacacacagc acagaaggta cggcgatgtc
     3901 cgagcggagc cgaaagacgt cgaaggtcag cgaggagtct gcgaagtacc tgtcgaacca
     3961 gggcccaacc acggggtcat cacggtagtt cagccgggtc ggcggcagtc gacgcaccca
     4021 ggtgagcatg aacgcgtcgc gctcgatcac ctcatacaaa ccgctgagca gtgcaagatc
     4081 ggcgtcgctg tgacaggcct gccctgaaga caccgacagg gcaaccatgt cgctgtgttc
     4141 gacgagcctt ggcacgtagg ggatgtagac caaacaggcc ggaacgaagc gcggctcgcc
     4201 gtctcggatc cgcgcggccg gcacccaggt catcggaagg tcctcggtat gccgcgcgaa
     4261 agggaactgc ggatgcgcgt actggcgctc gtcgaagagc tcgaagtcat ccacgagcag
     4321 ggccccctcc ccgagttgcc gtgccggagc ggtgaggagg ttcggcggct ggacggcgca
     4381 gcagtaccgc tcgacacatt cgccgatcgc gctggctgca gcctgctccc aggtctcggc
     4441 catcgctccc gtgtgtccgg ccatctccgc accagcgatg gacgtcaacg agttcgggac
     4501 gcttgcgaca ctgaagagtc ggggctcgcc cggagcgttg aggatggtcc gcaccgcgcg
     4561 cacgatcgag gtgggcccca ccatccagtc cagggcccca cccaagtcct gacgtgattc
     4621 aacgcccatg tcgtcagctc caagtcctct cttgaggaac tgaactcacg gtgctgcagt
     4681 gcgggcaccg aggcagtttg agcacggggt gggtctccag gcgcagctcc cacaggtcga
     4741 aggccagtag cgatccggcg gttcgaacga agacatcgcc ggccgctaga agtcggacac
     4801 cttccagagc gaccatcgct ccggtgaacg cggtgacgct cggcagactg ccgtgctcga
     4861 cgtaccgctc cagtgaagcg acgtagtcct gaaactcctc gtaccgcacc accgaactga
     4921 gattgctctt gaaccggaac tcaaagcact tccaacacgc cgtctgccca gggatcacgg
     4981 acggccccac gtggacgttc gccctctgca agagggcacc ggtcgtccac ggcgtgccgg
     5041 cggcgatggt cgcccgattg aactcatcca tccacggcag cgacacgacc ggggcatcct
     5101 gaacaacaac ggcgacatcg atgtcgccca gcagttgctg ccagcccaac ttgctctccg
     5161 ggaatggcgc gggctcgacg agggtgggaa ggtcattccg cttcaagacc gccgcgacga
     5221 cctcacccat ggaccgatcc agatcggtgg ggccgatgtg cgtggactgg ccgacgtcga
     5281 ctgcacccac ggtccgccga gcggcgacga caatgcgtcg cacaccactg gccgcgaggc
     5341 ccacggccac gtcgggcatc agcggacctt cgccggcaag gagaaccgag ccagaggcga
     5401 tccgtgcagt cgtgccgtca tcgccgccgt gctggcgcat gaaccggtcc tggccccgca
     5461 gcttcggcag gagctgcgag gggaccccct ccaactcggc cgcgggttca gcgaactcga
     5521 cgacccccat gtccagcagt tgccgcaggc tggcctccag gttggggaac tccccgtcgg
     5581 aggcgagttc ggtcagcacc ccttccaaat cccgcgtccc gtccagcagg ggaagcagtc
     5641 gtgggaagac ctccgagacg aggcggcccc gaaggactgc gctccgccga ggggtccgca
     5701 tggtcaagcc cccgtctgcc gcggggacga ggtagacctc ccccaaccga aggttgcctc
     5761 gctgaggcgc ggatgcggtc atggtcacgg cggaactcct tgttggacag cgggcggggg
     5821 agaatgcgag gacggttcgc cgctgtgggg cacagaccgc tccacagcgg cgttccgctt
     5881 gctcactcag aaccagacga cgcaactgca cgacgtgcag ccgcagctgg tggtgcacgc
     5941 acatgcacca ccgaggagtt cagtccgctc ctcaaggtct tcgatctcga gcaggtccgt
     6001 ctcgttgatt tccatccgat tcaccccctc ccagggacat ccgccggcct ggatggtcgg
     6061 ctaccaggtg tagacgcagg tgcaactcgt gcacgaacag ctgcacgagc acccgcacga
     6121 ccagctgcca ggggtctcgc gctgctcaag ctcctcgatg ctgaacatct cgtcgacgag
     6181 ctcgacctcg gtggaccgat tcatgtgtta ctccaatcac gggacgaaat ggcacacgtt
     6241 caggaacacg cgaatggacg gttaggccca cgcaacgcaa cttgtggagg tgcagccgca
     6301 actgcaggtg caggcgcaca cgccgctgat caactctgat cgctcctcca gatcttcgat
     6361 ctgcaaggca tcctcttcga ggacgatgtc gtgaaccatc gactccacta tgtcacctcc
     6421 ttcctcggtc tattactccc gggtcacggc gttgatttca tcgtcgatct cagccagaga
     6481 tagagttccg gcaacaacct tccgaatcgt tccggttcgg tcgatgaaat accacgtcgg
     6541 cgtgacgcgc aatgccaggt aggtcgcgat cgaggagtcg gtgtcgacga tcacggggaa
     6601 cggcaccgaa tccagggaaa gggcctcgct gggtggccag gaaaggaacg ggagatggcg
     6661 agtggacacg atcacgatgt cggccttggg ccggaaggtg cccgccgcgt gcccctgaga
     6721 catgagcgcg aggtcggcct tgcagtactc gcaccaatgg gcgacgacga gcagaacgac
     6781 ggggtgcccg gttggcactg agacgggctg gttggcgtag ttgacacccg aaatgctcgg
     6841 tatgaccgaa ttgacctccc cgccaggccc ggatccacca accatgatcg gatcgtcggt
     6901 gtgggtaagc agtccgggct tcatccccag cgttccgctg accaccgcaa ggccaagtcc
     6961 gatgattgtg acaaacagta ctattcggac cacgatgcgg cgtcgtcgca tcgtgcgctc
     7021 ggcttcggtc acgtcccccc gtggactaga catggctatc cgccggcgga ggcaatgacg
     7081 atggtcaact cactggcgga cggcaacgct gtgtcatcga tattgatcac ccgatatccg
     7141 tccgcaaata tcagcgttga tcgcggcacg gtgcgatccg gggccctcac gtatgcgctg
     7201 acctcgggaa gccggtcgca taaatcgtcc agggctgcac ggactgtggc gccggaacat
     7261 cgaacctggg gtggtagtcc atatcgcgcc cacacagctt ggggcaggca gatgtcgtgc
     7321 tcgtccatgg acgaaacctc tgtcatgtat cggggggaga tcctctacgc tgccaacgta
     7381 acacggcacc gttggacccg caacgcgaat cggaaagcgg ccgaactaca cccgcgcgac
     7441 acatcagtgt ttggtccgcc gaaactgtcg gaacgtgtaa tgatgcgcat aaacggctgc
     7501 ctcggggtcg cacattcgtg atggcgcacc gccgtcggcg gaccgcgagg tgcccgacct
     7561 gcgactcagc gggagtcggc ccggtcgtcg ggggtagccg ggtcgaccgg caacgggccc
     7621 acggcataca gggggtcgtc ctcgctgccg gccaggatcg aacggaacgc ttcgaggttg
     7681 gccaacgact caccgcgagc gacccgccag gcccactcgc gcttcatcga cgtgagaaag
     7741 ccgatcgcca gcaggtcgtt gaacggctca tcggcggcct ccagcagcgc ccccaccagc
     7801 agatccagat accgcccatc cagcgccacc gtcggcacct ggccacggac atagacgtca
     7861 ccagagccgt cgacggcata ccccagcagg gggcgacgca tcacccgccg cagcaggaac
     7921 cgatagaact ccgcgtggtt ctcgtcaggg ttgcggatga cgaaggcgct caccgacgtg
     7981 agctcgtcgc gcacgaggaa cgacgcgacg gtcttgagct tgcgctcccc cggaatggtg
     8041 acgaccactt ccccgtcgcg cgcgccgggc tcgctggtca gctcggcatc ggcgacgtat
     8101 gccgcgagcg cggccaccgc gcgctgccca ggcgtggcgt cggccgggtc ggtcacggga
     8161 cgaccgccgc cgtcaagtcg tgttcttggg ccacgatcac aacccgcgcg ccgtatcgac
     8221 cagaatgtct cgcttcacga gatgccggtg ttgacgacgg cacccgtgcg cgccgcttct
     8281 tgcaccgcgt cgcacacctg aatcgtcttg agcccagcgc gcccgtcgca tatcggctcg
     8341 gcttcgccgc ggatcactgc ggcgaagtgc gcgatctggt ttgccagcgg atcttcgacg
     8401 gcaacgtcga tgcggctgtc gtcagtggca gaccaccacg actgcactcc gggatacgtc
     8461 ttgagtcgca tggtcgggat cgagagcgac ccaaccgtgc ccatgacgtg atagctgtcc
     8521 tcgtccggat aggtggcgta ggaggtgttc tccccgctgg tttgctccca tgatcgagga
     8581 gaggctgcgg tatcggagag catgaaggtg ccgagcacgc cgccagcgaa gtgaaagatc
     8641 atggcggctg tgtcttcgac ctcgaagcct cgggtcgcag agcttgtggt ggcctggaca
     8701 gcggtgatgt caccggccag cgccatcagg ttgttcacct cgtgcgtgag gttgagcagg
     8761 atcgggccac ctccggcctt gcgccgccac ccgtctccga cgtcgaagta gtcgtccggc
     8821 ttgtagaacg tggcctgacc gacgatagcc accaagcgtc cgagtacgcc tgaactgatc
     8881 acctcacggg cggcggccat gttggcggag tagttgcggt gatggccggt gagcagcgga
     8941 acgtgagcag cctctgcggc ctcgacgagc tgcttggccc cggcggcagt ctcggccaaa
     9001 ggcttctcga cgatgaccgg caccccagcc gcgatgcagg tcaagccgcc ttcgacatgg
     9061 aggcgattgg gggtggcgag gatcacgccg tcgggccgat cgtcggccag cgctcgctcg
     9121 agagtgacgt aatgaggcac cccgagctcg gcggcgaggg caccggcttc cggcgacgga
     9181 tcgatgatcg aggccaagct cgcgtgcggc gacttgacga gctcttcgat gtgacggcgc
     9241 ccgatcagac ctgctcctgc gacggctatg cgcatgtcct ggagactacc gaattgctgg
     9301 cgcggaatcg gcccagatgt cggagtcggg gctgaatttc atgctggtca gaaatggctc
     9361 gcagccgggt ttgcccatcc gccagcgggc caggccgggc cggtcacggg acgaccgctg
     9421 ccggcactcc cgtcaacagg ctcgcctcga agatcggtga cggggtccgt gtgcgacatg
     9481 cttcgccgta agcctgcagc agccgctcgg tcgtggctcc ccacccgaaa ctgcgcgcgt
     9541 ggcgcacggc gcggcggccc aactcggccc gcaggccggg gtcggcgagc aggcggtcga
     9601 gggttgtcgc ccagtcatcg acatcgtgcc catcgacgag cactccccca ggaccgaccg
     9661 ccgtcggcag cccgccgacc cgcgcggcaa ccaccggggt cccgcacgcg agcgactcga
     9721 tcgcgaccag gccgaacgac tcgttgtagg agggaacggt caccaggtcg gacgcgcggt
     9781 accactgcgc gagagtcgcc cggtcggccg gcggcacgaa gcgcacgtat gccgtcatcc
     9841 ccagctccgc ggccagcgtg gccaggctct cggggtgttc caaaccggtg ccgctcgggc
     9901 caccgaggat gccgacgacc aggtcgtcgg gcgtccagtc ggggtgcgcg tcgagcagcc
     9961 gccgggccgc cttgacgagc aggtcgggag ccttgagcgg ctggatgcga cccacgaaga
    10021 ggaccacgtg cgcaccctgc gggagcccga ggcggctgcg cgcggcatac cggtcgccgg
    10081 gggtgaagac ctcgaggtcg acgcccgggg ggacgacgac gacgcgggcc gggtcggcgt
    10141 cgtagagcga caccagctgg caggcttcgt cggcggtgtt ggcggtgagc aggtcggcgg
    10201 cttcgacgac ctgggcctcg ccgatctcgc ggccggccgg ctcgggacgc tcgccgtcgg
    10261 cgagcgcggc gttcttgacc cgggccatcg tgtgcatcgt gtggacgagc gggacgcgcc
    10321 accggtcggc cgccagccag ccgacctggc cggagagcca atagtgcgag tggacgaggt
    10381 cgtaccagcc ttcagggcgg gccgcgccgg cgcgcatgac gcctgcggcg aacgcgcaca
    10441 gctgaccggg aaggtcgttc ttggccaacc cctcatacgg ccccgcggtg atgtgccgga
    10501 cggtaactcc ggggacgagc tcgaccgtgg gcgggagggc gccggtcgtg gcccgcgtga
    10561 agatctcgac ctcgcggttc tggcgggcca actccttggc gacctcggcg atgtagacgt
    10621 tcatcccgcc
//
LOCUS       DAOMZY010000090        10569 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5891_length_10569_cov_4.434659, whole genome shotgun sequence.
ACCESSION   DAOMZY010000090 DAOMZY010000000
VERSION     DAOMZY010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 10569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10569
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5891_length_10569_cov_4.434659"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="PLK64_06800"
     CDS             complement(<1..722)
                     /locus_tag="PLK64_06800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPV79487.1"
                     /translation="MLESQALTAAVVIITYRRPHFVRTCLEHLAALTRAPEETYVIDA
                     SPDGLTRDVVADFPTVRYLHSDAGPGTMATSRAMALAATTCDVLVFLDDDANADPDWL
                     KQLLLPYADPTVAAVGGRARNGQPGEETEGLDQIGLLTADGKLLGFFAADPGHDVDVD
                     HLLGANMSVRREVLLALGGIRDNYPGTCLREESDMFLRVRQAGHRIVYTPSAVVTHVA
                     APYVHGRRFDLRYHYYGHRNHIV"
     gene            complement(736..1740)
                     /locus_tag="PLK64_06805"
     CDS             complement(736..1740)
                     /locus_tag="PLK64_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79488.1"
                     /translation="MQNHNVGSTQGWRTAYLVLTHTDAAAVVRLVAAIRRSSPRCVVI
                     VAHDARVQPPPAFADEHVLVWCHGLATDWGSWELVEATLGAVAQARAFADPDFFVLVS
                     GQDYPARHLGTWEDEVRAAGGGWIGRPRPLRYRPTWGRRPGVGDDDLTRYSFRWWPLP
                     PAVARMGRRFPERARHGAARVRDAVLLRAAPVVAVRFVSRGRGAHLGLPRSRRALGGR
                     VFVKCDQWIAFDRDLAAVVLRDLAAGMPLRRLFERSIIPDESAIQTALHAVEPPTIPH
                     PVSFVRWLPSLDRPAVLTLADLPELQASRSPFCRKVSSGHSADLLAALDDLVTGAGAR
                     "
     gene            1787..2809
                     /locus_tag="PLK64_06810"
     CDS             1787..2809
                     /locus_tag="PLK64_06810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPV79489.1"
                     /translation="MPAPGFTLVIPTYHRPDLVAEAVASALAQVVPFARIIVVCDGDQ
                     PAVGGRLAGLPVDVVTVAHGGVAAARNHGLSLVDTSWVCFLDDDNLLHPAYLASVQEF
                     VSTRDAVQALNTYYWVFATDFLPGVDVVAHNLAECLAAAATETPVNDLSYLHIAGQSY
                     RLLLERLRGSVSGCAITTEVLRAAGGFPAGRTCAEDWTMFVNVARLTEWQVIEDRLVF
                     FRDHPLTNTRTGLSANGVDTVRAMHEFWTDPRLHEPCPVDLGDYRAAYRTTVRFALEA
                     ARVDGSSGRFAEIQRLAPVVLPRRVDRILARVPPRWWDRLRVARSLIGVPRREADARV
                     AAKSVG"
     gene            2828..4039
                     /locus_tag="PLK64_06815"
     CDS             2828..4039
                     /locus_tag="PLK64_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide ABC transporter ATP-binding
                     protein"
                     /protein_id="HPV79490.1"
                     /translation="MSAATAISVRGLGKTYRIAHASDRPTRATEAIVARLRHPLRRTT
                     FDRFEALTEVSFDVAAGTALGIIGRNGAGKSTLLKILTRITPPTRGQAELAGRVGSLL
                     EVGTGFHPELTGRENVFLNGTLLGMRRQEITRRFDEIVDFSGCGQFLDTPVKRYSSGM
                     YVRLAFAVAAHLETEILVIDEVLSVGDADFQRRSVQKMRDAAAQGRTVLYVSHQLHTV
                     TALCSEALLLDRGTVSAYGAVEDVLATYRASVAEHAVVQRETRAQVPAGILHVVAATT
                     TRLTYLPAEPKRFTFTIQAEAAPDGPYFLSCHINDANGGVVAQCDSRLGGLWFHPDQP
                     QEVTLDLRQPWLKPGTYTVDVFLCRVGIVDVWDGAVTFDVEQELPYAVTGTAESFGRG
                     MVLADFDFGVR"
     gene            4039..4860
                     /locus_tag="PLK64_06820"
     CDS             4039..4860
                     /locus_tag="PLK64_06820"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV79491.1"
                     /translation="MAPHTVIGPPGPLHLPRWSELWEAREVLWRFGQRDILLRYRQTL
                     IGVAWVILQPIVTAGVFALVFGRVAQLPTGGIPYFLFGYVGTLLWTVFASTVNRAAGS
                     LVSNQALVSKVFFPRLLVPLSTCVSVLVDFLVALVIGFGLLWWFGVWPGWAILTIPVW
                     VTLCVALAMGIGLAAAAIMVRYRDIAYVLPWAVQTLLYASPVAYSLEAVPDAVRPYLL
                     ANPLTWVLEGMRWAMLGSAAPPVWQIVGAVVVSGLVLFAGLLVFQRYEREFADLI"
     gene            4888..5838
                     /locus_tag="PLK64_06825"
     CDS             4888..5838
                     /locus_tag="PLK64_06825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HPV79492.1"
                     /translation="MSLRRVDVILASNRGGPFLDEAIDTALDQEGVSTRVILVDDGSP
                     DPEFMERAAARREGVDLVRQPPQGVSVARNTGLDRVQADLVAFLDDDDIWEPTLLTTL
                     VGALASDPLALGAYSGGYYLDAAGNHFGQGWSAAPARREDLLRGAVPIPTIITLLLKA
                     QACRAVGGFDPGFDFGEDNDFILRLLQRGPMVGVDLQLVGYRRHQHNATNGDPRVRAA
                     ASDRLIRLNRTRALASGDTDTARLLTENIARFRAGVAQGFGADLPRQVKSRQFGEAWS
                     GLREGFSTNPRTFTAGVLGSVVRYSRRSRQRDPRVGNDLG"
     gene            5835..6707
                     /locus_tag="PLK64_06830"
     CDS             5835..6707
                     /locus_tag="PLK64_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79493.1"
                     /translation="MKLNAYVLAADPAWIEESIAGYYPHVDRIIVSFDETGTSWTGHP
                     LDPTEAVSRLKALDHQGKIVLAPGRFFSLGRNALASDTAQRQNALDLASEGAAWVLQL
                     DSDEIAADPATLLSCLEQADREGFDALDYPARYVYQHIRDRWYLERGRRLWRAAAAYP
                     GAVAVRAGTRLRLSRQTDAALFRVDFSRRNTDPWHPPSAIVHRVVHPGQAIMHLSWVR
                     SESAMLAKTSTWGHAGERDWSADVLQWSRRGRRPLRTVAAGQLSRNPDNWLRFLRLAD
                     TPDAAARARNGLTS"
     gene            complement(6714..7367)
                     /gene="pth"
                     /locus_tag="PLK64_06835"
     CDS             complement(6714..7367)
                     /gene="pth"
                     /locus_tag="PLK64_06835"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPV79494.1"
                     /translation="MATWLVVGLGNPGPRYAGNRHNVGAMVVAELARRYAGGSGSTPS
                     SGAPVVTTSLEHLKAHKARAHVAAVRLGTLPGGAPGPAAALAVPTAYMNESGGPVKAL
                     AQFYSTPLEQLVVVHDDLDLTAGTIRLKLGGGEGGHNGLRSITGAMGSRDYLRVRVGI
                     GRPPGRMDPADFVLRDFAPTERAELPFTVSDGADAVELLIQQGLVAAQQRVHAPGPL"
     gene            complement(7379..7987)
                     /locus_tag="PLK64_06840"
     CDS             complement(7379..7987)
                     /locus_tag="PLK64_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HPV79495.1"
                     /translation="MSEIKLPAERRNSFGKGAARKIRRDHKIPAVLYGHGTDPVHITL
                     PAHDTVLALKHANALLSIELEGKSQLALAKDIQRDPIRPIIEHIDLVIVRKGEKVNVD
                     VPVHIEGEAGPDTIVNVESNTIELLVEATHIPESVVVSVEDLPAGTQIKAGELVLPEG
                     ATLVTDEDALIVNITGLSAQEPDADAEAEPAAAATATADAAE"
     gene            complement(8214..9071)
                     /locus_tag="PLK64_06845"
     CDS             complement(8214..9071)
                     /locus_tag="PLK64_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79496.1"
                     /translation="MPLSGDERVPPSAPGGPPTIALLHATYHRPGGVGPLRDAWLGRA
                     DRADLVDYIVAMDGDDPAVRDTAGWHRVVGPPSDGTVSAVRNWNAAAAASSADLLVVL
                     ADDLFPEPGWDTTLRRMLDGWDPQAVAYAVKITDSPDPDDALLRHPIVSRRFYVTSGL
                     FAPQLRGVYCDDEITRRAYWRALILDGRRLAFDHAHPTLTGQVETTSHHLVNRDTEHA
                     LGVRVYEGLWPKWRRRAAIRLAPTSGPLVGHPRAVRGLVLTRAAVDVAGWLVRAAVQR
                     AEQVLRRRP"
     gene            9130..10275
                     /locus_tag="PLK64_06850"
     CDS             9130..10275
                     /locus_tag="PLK64_06850"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPV79497.1"
                     /translation="MTDAQPTGPRATRRYAYIDALRGFAALWVFVLHVHGYWLDDVRP
                     PKLSADGLLVRLIGFGGAGVDLFIVLSGFCLTLPLTQAGTTALARIDVRGFFRRRAFR
                     LLPAYYAALAVVVAIELIPAVRPALVPRDLTGVDVLTHLTLTFPLWQSTLAAVNGSLW
                     SIPLEATLYLGFPLLVWLAGRRGIRAVLVMTVAVAALWAVIIGSLSWPLPGGWLPQPD
                     KYLPARWLEFAMGMYAATAVRGIPHPRATTHAVWACALGLPAGLYGYAAGTDLVRTLG
                     FGALGAGLLILLQRVPERHFGAGPLAWLTGLGTISYSFYLLHQPVILLLSGWGHNLGL
                     GLIPLFVVALATSGTLTVAVATAFYRGVERPFLVRGSMSAVVRGTTT"
     gene            complement(10278..>10569)
                     /locus_tag="PLK64_06855"
     CDS             complement(10278..>10569)
                     /locus_tag="PLK64_06855"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144949.1"
                     /note="catalyzes the formation of 5-phospho-alpha-D-ribose
                     1-phosphate from D-ribose 5-phosphate and ATP; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HPV79498.1"
                     /translation="NTTDTGGTITKAADAVMAEGAAGVIVAATHAILSGPAVDRLRAS
                     SAREVIVTDTLPIPIDRQFDKLTVLPIAPLISRAIMEVFEDGSVTSLFEGRA"
BASE COUNT         1526 a         3662 c         3673 g         1708 t
ORIGIN      
        1 acgatgtggt tgcggtgtcc gtagtagtgg tagcgcaggt cgaagcgccg cccgtgcacg
       61 tagggcgcgg cgacgtgcgt caccaccgcg ctgggcgtgt agacgatgcg gtgaccggcc
      121 tggcgcaccc gcaggaacat gtcggactcc tcgcgcaggc aggtgccggg gtagttgtcc
      181 cggatgcctc ccagggccag caggacctcc cgccgcaccg acatgttggc gcccaacagg
      241 tggtcgacgt cgacgtcgtg gccggggtct gcggcgaaga agcccaggag tttgccgtca
      301 gccgtcagca gcccgatctg gtccaggccc tcggtctcct cgcccggctg cccgttgcgg
      361 gccctgccgc ccactgcggc gacggtcggg tcggcatagg gcagcaggag ttgcttgagc
      421 cagtcggggt cggcgttggc gtcgtcgtcg aggaacacca ggacgtcgca ggtcgtcgcc
      481 gccagcgcca tggcccgcga ggtcgccatc gtccccgggc cggcgtcgct gtgcaggtaa
      541 cggacggtgg ggaagtcggc cacgacgtca cgggtcagcc cgtccggcga cgcgtcgatg
      601 acgtaggtct cctcgggcgc ccgggtcagc gcggccaggt gttccaggca ggtgcggacg
      661 aagtgcggcc tgcggtaggt gatgatgacg acggcggccg tcagggcttg gctttcaagc
      721 atgcttcgca gtctctcatc tggcgccggc tccggtcacg aggtcgtcca acgccgcgag
      781 cagatctgct gagtgacccg acgacacctt ccggcagaac ggcgagcgcg aggcctggag
      841 ctccggaagg tcggccaagg tgaggacggc gggccgatcg agcgacggaa gccaccgcac
      901 gaaactcacc gggtggggga tcgtcggcgg ctccaccgcg tgcagcgccg tctggatcgc
      961 cgactcgtcc gggatgatgg accgctcgaa gaggcgacgc agcggcatac ccgcggcaag
     1021 gtcgcgaagc acgacggcgg cgaggtcgcg gtcgaaggcg atccattggt cgcacttgac
     1081 gaacacccgc ccgccgagcg cccgccgcga tcggggcagc cccagatgag cgccccggcc
     1141 gcgcgagacg aaccggacgg ccacgacggg agcggcccgg agaaggaccg cgtcgcggac
     1201 ccgggccgcg ccgtggcggg ctcgttccgg gaaccgtcgg cccatccgcg ccacggccgg
     1261 gggaagcggc caccaccgga aggagtagcg ggtcaggtca tcatcaccca cgcccggtcg
     1321 gcgtccccag gttggccgat atcgaagagg gcgcgggcga ccgatccacc caccacccgc
     1381 tgcccggacc tcgtcctccc acgtcccgag gtgccgcgcg gggtagtcct gccccgagac
     1441 cagcacgaag aagtccgggt cggcgaaggc cctcgcctgc gccaccgccc cgagggtcgc
     1501 ctcgacgagc tcccaggacc cccagtcggt cgccagcccg tggcaccaca cgaggacgtg
     1561 ctcgtccgcg aacgccgggg gtggctgcac gcgggcgtcg tgcgccacga tgacgacgca
     1621 ccgggggctc gaccgacgga tcgcggcgac cagccggacg accgccgccg cgtcggtgtg
     1681 ggtgagcacg aggtatgccg tgcgccaccc ctgcgtcgat ccgacgttat ggttctgcac
     1741 gaccgtcagt attcccgcgt cgcccccgca tgacatcatc agggcaatgc ctgctccagg
     1801 attcacgctc gtcatcccga cctatcatcg cccggatctg gtggccgagg cagtggcgag
     1861 cgcactcgcc caggtcgtgc ccttcgcacg gatcatcgtc gtctgtgacg gcgaccagcc
     1921 tgccgtcggg gggaggttgg ccggcctgcc cgtcgatgtc gtcacggtgg cgcacggtgg
     1981 ggtcgcggca gcccgcaacc acgggctctc cctggtcgac acgtcctggg tgtgcttcct
     2041 cgacgatgac aacctgctcc acccggccta cctcgcgtcg gtccaggagt tcgtgtcgac
     2101 ccgcgacgcg gttcaagcac tcaacaccta ctactgggtg ttcgccaccg actttctccc
     2161 cggcgtggac gtcgtggccc acaatctggc ggagtgcctg gctgcggccg ccaccgagac
     2221 accggtcaac gatctgagtt atctgcatat cgccggccag agctaccgcc tgctgctcga
     2281 acggctgcgc ggttcggtga gcggctgcgc catcaccacc gaggtcctgc gggccgccgg
     2341 ggggttcccg gcgggccgca cctgcgccga ggactggacc atgttcgtca acgtcgcccg
     2401 gctgaccgaa tggcaggtca tcgaggaccg ccttgtcttc ttccgggacc acccgttgac
     2461 gaacacccga accgggttgt ccgccaacgg tgtagacacc gtgcgggcca tgcatgagtt
     2521 ctggaccgac ccgcgcctgc acgagccctg cccggtcgac ctcggcgact atcgcgcggc
     2581 gtacagaacg acggtccggt tcgcgctgga ggcggcgcgg gtggacgggt ccagcgggcg
     2641 gttcgccgag atccagcgac tggcgcctgt cgtgctcccc cgccgcgtcg accggatcct
     2701 cgcgcgcgtc ccgccgcgat ggtgggaccg gttgagggtg gcgaggtcgc tcatcggggt
     2761 ccctcgacgc gaggccgatg ctagggtcgc ggcaaagagt gtggggtgaa gggcgggtac
     2821 gggagctatg tcggcggcta cggccatcag cgtccgcgga ctgggcaaga cctaccggat
     2881 cgctcacgcc tcggaccggc ctactcgcgc gaccgaggcc atcgtggctc ggctgcgcca
     2941 cccgctgcgt cggaccacat ttgatcgctt cgaggcgctc accgaggtga gcttcgacgt
     3001 cgccgccggc accgcgttgg gcatcatcgg ccgcaatggc gccgggaagt cgacgctgct
     3061 gaagattctg acgcggatca cgccgcccac gcggggccag gccgagttag ccggccgggt
     3121 cgggagcctc ctcgaggtag ggacggggtt ccacccggag ttgaccgggc gggagaacgt
     3181 ctttctcaat ggcacgctgc tgggaatgcg gcgtcaggag atcacccggc gcttcgacga
     3241 gatcgtcgac ttctccggat gtgggcagtt ccttgatacg ccagtcaagc ggtactcctc
     3301 agggatgtat gtccgattgg cgttcgccgt cgctgcccac ctcgaaaccg aaatcctcgt
     3361 catcgacgag gtgctcagcg tgggcgacgc cgacttccaa cggcgctcgg tgcagaagat
     3421 gcgcgacgcc gcggcccagg ggcggaccgt cctgtacgtc agccaccaac tgcacaccgt
     3481 cacggccctg tgttccgaag cactgctgtt ggaccgtggc acggtgagcg cttacggggc
     3541 cgtcgaggac gtgctagcca cctatcgggc gtcagtggcc gagcatgccg tcgtccagcg
     3601 agagacccgc gcacaggtgc cagccgggat cctgcacgtc gtcgcggcga cgacgacccg
     3661 cctgacctat ctgccagccg agcccaaacg attcaccttc acgatccagg ccgaagcggc
     3721 gccggacggg ccctacttcc tctcctgcca catcaatgac gccaacggcg gcgtggtcgc
     3781 gcagtgcgac tcccggctgg gcgggctgtg gttccacccc gaccaaccgc aggaggtgac
     3841 ccttgacctg cgccaaccct ggctcaagcc cggaacctac accgtcgacg tcttcctgtg
     3901 tcgggtgggg atcgttgatg tgtgggacgg cgcggtcacc tttgatgtcg agcaggaact
     3961 gccgtatgcc gtgacgggga ccgcggaatc cttcggtcgg ggcatggtgt tggcggattt
     4021 cgatttcggt gtgcggtgat ggcaccgcac accgttatcg gtccccccgg gcccctgcac
     4081 ctgccacgct ggtcagagtt gtgggaggcc cgcgaggtcc tctggaggtt cggtcagcgg
     4141 gacatcctcc tgcgctatcg ccagacactc atcggggtgg cttgggtcat cctgcagccg
     4201 atcgtcaccg ccggggtgtt cgcgctggtc ttcgggcgcg tggcccaact ccccacgggg
     4261 ggcattccct acttcctctt cggttatgtc ggcaccctgc tctggaccgt cttcgcgtcg
     4321 accgtgaacc gagcggccgg ctcgcttgtc agcaaccagg ccctagtgtc caaggtgttc
     4381 ttcccgcggt tgttggtgcc gctgtcgacc tgcgtgtcgg ttctcgtgga cttcctcgtc
     4441 gcgctcgtga tcggcttcgg actgctgtgg tggttcggtg tgtggcctgg ctgggccatc
     4501 ctcaccatcc ccgtgtgggt cactctgtgc gtggctctcg cgatggggat tgggctcgct
     4561 gcagcggcca tcatggttcg ctatcgcgat atcgcctacg tcttgccgtg ggccgtccag
     4621 accctgttgt atgcctcccc cgtcgcgtat tccctcgaag ccgttcccga cgcggtccgg
     4681 ccctacctcc tggccaatcc cctcacctgg gtgctcgaag ggatgcggtg ggccatgctc
     4741 ggctcggccg ctcctccggt ctggcagatc gttggcgccg tcgtcgtcag cggtctcgtc
     4801 ctgtttgccg gactgctcgt gttccagcgc tacgagcgcg agttcgcgga tctgatctag
     4861 atggaagaga acatcgagga cgatccgatg agcctgcgcc gtgtcgatgt catcctcgcg
     4921 tcaaaccgcg gcggcccttt cctggacgaa gccatcgaca cggcgctgga ccaggaaggc
     4981 gtgtcgaccc gggtcatcct cgtcgacgac ggctcacccg acccggagtt catggagcgg
     5041 gcagccgccc ggcgggaggg cgtcgacctc gtccgccagc cgccacaggg ggtgtcggtc
     5101 gctcgaaaca ccgggttgga ccgggttcag gcagacctcg tcgccttcct cgacgacgac
     5161 gacatctggg agccaaccct gctcaccacc ctcgtggggg ccctggccag cgaccccctg
     5221 gccctcggcg cctattccgg tggctactac ctcgacgcgg cagggaacca cttcggccaa
     5281 gggtggtcgg cagcaccggc gcggcgggag gacctgctgc gcggcgccgt tccgatcccc
     5341 acgatcatca cgctgcttct caaggcgcag gcgtgccggg cggtgggtgg tttcgaccct
     5401 ggtttcgact tcggggagga caacgacttc atcctccgcc tcctgcaacg cgggccgatg
     5461 gtcggcgtcg acctgcaact ggtcggctac cgccgccacc agcacaacgc caccaacggc
     5521 gacccccggg tccgggcggc cgcatccgac cgactgatcc ggctcaaccg cacccgcgcc
     5581 ctcgccagcg gcgacaccga caccgctcga ctgctcaccg agaacatcgc gagatttcgg
     5641 gcgggggtcg cccagggatt cggagcggac ctgcctcgcc aggtgaagtc tcgccagttc
     5701 ggcgaggcgt ggagcggcct gcgcgagggc ttctccacca acccgcgaac cttcacggct
     5761 ggagtgctcg gctctgtcgt tcgctactct cggcggtccc ggcagcggga cccacgggtc
     5821 gggaacgacc tcggatgaag ctcaacgcct acgtgctggc agccgatccg gcctggatcg
     5881 aagagagcat cgccggctac tacccgcacg tggatcgcat catcgtcagt ttcgacgaga
     5941 cggggacgtc gtggacgggg caccccctcg acccgacgga ggcggtgagc cgtctgaagg
     6001 cgctggacca tcagggcaag atcgtcctcg cgccgggcag gttcttcagc ctgggacgaa
     6061 acgccctcgc cagcgataca gcccagcgcc agaacgccct ggacctggcg agcgagggtg
     6121 ccgcctgggt ccttcaactg gactccgacg agatcgccgc cgaccccgcc acactcttgt
     6181 catgcctcga acaggctgac cgggagggtt tcgacgcctt ggactaccca gcccgctacg
     6241 tctaccagca catacgggac cggtggtacc tggaacgtgg ccgacggctg tggcgcgctg
     6301 ccgcggcata ccccggggcc gtcgccgtgc gggcggggac ccggttgcgc ctgtcccgcc
     6361 aaaccgacgc ggccctgttc cgcgtggact tctcgcgccg gaacaccgat ccctggcatc
     6421 cgccgagcgc gatcgtgcat cgggtcgtcc accccgggca ggccatcatg catctgagct
     6481 gggtgcgctc cgaatcggcc atgctggcca agacaagcac ctggggacat gccggcgaac
     6541 gggattggag cgccgacgtc cttcaatggt ctaggcgggg ccgtcgtccg ttgcgcaccg
     6601 tcgccgcagg gcagctgtcc cgcaaccccg acaactggct gcggttcctg cgattggccg
     6661 acacaccgga cgcagcagct cgggctcgca atgggctgac cagctgagtc gcctcagagc
     6721 gggcccgggg catgcacccg ctgctgtgca gccaccaggc cctgctgaat cagcagctcg
     6781 acagcatctg ccccatcgct caccgtgaac ggcaactcag cgcgctcggt cggagcgaaa
     6841 tcgcgcagga cgaagtcggc cgggtccatg cggccgggag gccgtccgat gccgacgcgc
     6901 acccgcaggt agtcacgcga tcccatggct cccgtgatcg agcgcagccc gttgtggccg
     6961 ccctcgccgc ccccgagttt gagccggatc gtccccgcgg tgaggtcgag gtcgtcgtgc
     7021 acgacgacga gctgctccaa cggcgtggag tagaactgcg ccagcgcctt cacgggccca
     7081 ccggactcgt tcatgtatgc cgtgggcacc gccaacgccg ctgccgggcc aggggccccg
     7141 ccgggcaggg tccccaggcg caccgcagcg acatgggctc tggccttgtg ggccttgagg
     7201 tgttcgaggc tggtggtgac caccggggcc ccggaggagg gagtcgagcc ggacccaccg
     7261 gcataccggc gggccagctc ggccacgacc atggcgccga cgttgtgtcg gttccctgcg
     7321 tagcgcggcc cggggttgcc caggccgacg accagccagg tggccatggc ggggtgggtt
     7381 actccgcggc gtcggcggtg gcggtagcgg cagccgccgg ctcagcctcg gcgtcggcgt
     7441 cgggctcctg cgccgacagg ccggtgatgt tgacgatgag ggcgtcctcg tcggtgacga
     7501 gggtcgcgcc ttcggggagc acgagctcgc cagccttgat ctgcgtgcca gccggcaggt
     7561 cctcgacgga gacgacaacc gactcgggga tgtgcgtggc ctcgacgagc aactcgatgg
     7621 tgttggactc gacgttgacg atggtgtccg gcccggcctc gccctcgatg tgcacgggca
     7681 cgtcgacatt gaccttctcg cccttgcgga cgatgaccag gtcgatgtgc tcgatgatcg
     7741 ggcggatcgg gtcgcgctgg atgtccttgg ccagggccaa ctggctcttg ccctcaagct
     7801 cgatcgacag cagcgcgttg gcgtgcttca gcgcgagtac cgtgtcgtgg gccgggaggg
     7861 tgatgtgcac cgggtcggtg ccgtgtccgt agagcaccgc ggggatcttg tggtcgcggc
     7921 ggatcttgcg ggcggcaccc ttgccgaacg aattgcgacg ctcggcggga agcttgattt
     7981 cggacatgac gctccttgca acagacgtga gcgacaacca cggaggtatg ccgcagggct
     8041 tgggcctcga cgcgggacgc gacacgcgcg gcagaggcga gccgactgaa cggcacacct
     8101 tgtcgatcac ggacgagccg tcatggcacg tccctcgccg aggcaactgt ccaatgctac
     8161 gggacgccag cagcgccacg aaatcgcggg gtccggctcg atcgcccggc acgctatgga
     8221 cgacggcgga ggacctgttc ggcgcgttgc accgccgctc ggacgagcca cccggcgaca
     8281 tccacggcgg cgcgggtgag caccaggccc cggaccgccc gcggatgacc cacgagtggg
     8341 ccgctggtgg gggccaggcg gatggcggcc cggcgacgcc acttgggcca cagtccctcg
     8401 tagacgcgca ccccgagcgc gtgttccgtg tcgcggttaa ccaagtggtg agaggtggtc
     8461 tcgacctgcc cggtcagcgt cgggtgggca tggtcgaagg ccagccggcg cccatcgaga
     8521 atgagggccc gccaataggc ccgccgggtg atctcgtcgt cgcagtacac cccccgcagc
     8581 tgtggagcga agagccccga cgtgacgtag aacctccggg acacgatcgg atggcgcagc
     8641 agcgcgtcgt ccgggtcggg cgagtcggtg atcttgaccg cgtaggcgac cgcttgcgga
     8701 tcccaaccgt cgagcatccg acgcaatgtg gtgtcccagc ccggctcggg gaacaggtcg
     8761 tcggcgagca cgacgagcag atcggcggac gaggctgcgg ccgccgcgtt ccagttccgc
     8821 accgcggaga ccgtcccgtc cgagggcggc ccgaccaccc gatgccagcc ggcggtgtcg
     8881 cgcacggccg ggtcgtcgcc gtccatggcc acgatgtagt cgaccaggtc ggcgcggtcg
     8941 gcccgtccca gccacgcgtc ccgcagcggc cccaccccac cgggccggtg gtaggtcgcg
     9001 tgaagcagtg cgatcgtcgg cgggcccccg ggcgccgagg gcgggacgcg ctcgtcgccg
     9061 gagaggggca tgacgcccat ggtaagtggc agtcgtcggg ccgcgaggga cggcgatatc
     9121 gtctggcgga tgacggacgc ccagcccacg ggcccgcgag ccacgcgccg atacgcctac
     9181 atcgacgccc tgcgtggctt tgcggcgctc tgggtgttcg tgctgcacgt gcacggctac
     9241 tggctggacg acgtgcgccc gcccaagttg agcgccgatg gcctcctcgt gcggctcatc
     9301 gggttcggcg gcgctggggt ggacctgttc atcgtcctgt ccggcttctg cctcaccctg
     9361 cccctcaccc aggcgggcac gaccgcgctc gcccgcatcg acgtccgcgg cttcttccgg
     9421 cgccgcgcct tccggctgtt gcctgcctac tacgcggcgc tcgcggtggt cgtcgcgatc
     9481 gagctgatcc ccgctgtccg ccccgcactc gtcccacgcg acctcaccgg cgtcgacgtg
     9541 ctcacgcacc tcacactgac cttcccgttg tggcagagca ccctcgctgc cgtcaacggc
     9601 tcgctctggt cgatcccgtt ggaggcaacc ctctatctcg ggttcccgtt gctggtgtgg
     9661 ctggccggcc ggcgcggcat ccgagccgtc ctcgtgatga ccgtggcagt cgcggcgctc
     9721 tgggccgtca tcatcgggtc gctcagctgg ccgctgcccg gcggctggct gccgcagccg
     9781 gacaagtacc tcccggcccg ctggctcgag ttcgcgatgg gcatgtatgc cgcgacggcg
     9841 gtgcgcggca tacctcaccc ccgcgcaacg acccatgcgg tctgggcgtg cgcgctcggc
     9901 ttgccagctg gcctctacgg gtatgccgcg ggcaccgatc tggtgcgcac gctcggtttc
     9961 ggggcgctcg gggcggggct gctcatcctg ctgcagcgag tgccggagcg acacttcggg
    10021 gctggcccgc tggcctggtt gaccgggcta gggacgatct cgtacagctt ctacctgctg
    10081 caccaaccgg tcatcctgct gttgtccggg tggggacaca acctcgggct ggggctcatc
    10141 cccctgttcg tcgtcgccct ggccacgtca gggacactca ccgtcgccgt cgcgaccgcg
    10201 ttctatcgtg gggtcgagcg accgttcctc gtgcgcggga gcatgtcggc ggtcgtgcgc
    10261 ggcacgacga cctgagctca ggcgcggccc tcgaagaggc tggtgaccga cccgtcctcg
    10321 aacacctcca tgatcgcccg cgagatgagc ggggcgatcg gcaggacggt gagtttgtcg
    10381 aactgtcggt cgatcgggat cggcaaggtg tcggtgacga tgacttcgcg agcgctcgat
    10441 gcccgcagtc ggtcgaccgc cgggccagag aggatcgcgt gggtcgcggc gacgatgacg
    10501 cccgcggcgc cctcggccat gaccgcgtcg gccgccttcg tgatggtgcc gccggtgtcg
    10561 gtcgtgttg
//
LOCUS       DAOMZY010000091        10493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_5975_length_10493_cov_5.608450, whole genome shotgun sequence.
ACCESSION   DAOMZY010000091 DAOMZY010000000
VERSION     DAOMZY010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 10493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10493
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5975_length_10493_cov_5.608450"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..736)
                     /locus_tag="PLK64_06860"
     CDS             complement(5..736)
                     /locus_tag="PLK64_06860"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HPV79499.1"
                     /translation="MSLDVRREVRRLLVIQHDHVSPLGPVAAAFEARGWECVYHLVVP
                     PERFDAPDVPGRFPPLSGVDAVLAMGAPWSVYEIELVGSWVLPEQDLLREADAGGIPV
                     LGICFGGQLLAQTHGGSVSRAPQGEIGWVDISTDEPEIVPPGPWFQWHADRWVVPPDG
                     VELARNEAASQAFSLRRNLGLQFHPEVTVDMLLGWLDNGGDAVLTAEGIDREDLLALT
                     RRLEADAAGRAERLVEGFLHFAGLA"
     gene            complement(733..2226)
                     /locus_tag="PLK64_06865"
     CDS             complement(733..2226)
                     /locus_tag="PLK64_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HPV79500.1"
                     /translation="MSSTNTPQGEHQLRKGSLGVLGIAFFVISAAAPLTAMAGGAPVA
                     MLLGNGAGIPFAYVIVAVLLLIFAVGYTAMARHHTSTGAFYSYVARGLGQRAGGIAAL
                     IALLGYNAMQIGLYGLFGAAASGFMEAQFGVAWPWWSYVLIAGVIIGFLGYRQVDLSV
                     KVLGVLVAAEFLIVVVLDLAVIIKGGQGGGAGLSMSSFSMDSLMSGSLAIALLFNSAS
                     FIGFEATTIYSEEARDPKRTVPRATYAAVLTIGLFYTVTTWLMVNAQGADGLVDHIGG
                     LTPDPTAFLFEISGIYLGELPTTIMTVLFATSVFAALLAFHNAVARYFFALGREGLLP
                     TPLGHTHKIHLSPHKGSVSQSVLALVVIVVFIVSGQDPVLALFTWLTQLGTLAILVLM
                     ALSSAAVTAFFARHKGLDTNPWRTTVAPIIGTVALGSVSVYAASQFGVLIGSPDSPLA
                     WALPSLVVVAALVGLALALRLRSTSPEQYAQMGRHRPSEVGLIDVGE"
     gene            2390..3934
                     /locus_tag="PLK64_06870"
     CDS             2390..3934
                     /locus_tag="PLK64_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HPV79501.1"
                     /translation="MTQHAPDHPPAAPLQDGFIAQVEGVRVDTRHWIGGRRVALATAG
                     AEGAVATFTTISPIDESPLAEVCLGGSAEVDAAVAAAQAAFPGWAALSVQERSEILRR
                     VADGIQARIEDLSRVETRDNGSLIRSHRRGVMPRVAMNFRFFADYAQDHLGHPDLTVP
                     GRGHRERISYDPAGVVAIITPWNAPLMLATWRIGPALAAGNCVVLKPPEWAPLTASLL
                     AEITHDAGMPPGVFNVVQGTGASAGAPLTRHPGINRLCFTGSVPTAGVIAAAAATNIV
                     PLSFELGGKSPLVVFDDADLDLAVEIAVEQFDNAGQVCLKAARMIVHESIAQEFTRRV
                     VDGAAKLTQGDPRDEATDVSALITRKHFEVVSGFVERAIADGARPLLGGGPNTDLGGL
                     YFRPTVLVDAPEDSEILTEEVFGPVVTIQTFATEEEAVARANDSRFGLAATLVTGDRD
                     RAERVSARLDAGTVWVNCFFVRDLEAPFGGNGKSGIGREGGVWSFDFYTDIKNSVFAP
                     HGWKDN"
     gene            3936..4910
                     /locus_tag="PLK64_06875"
     CDS             3936..4910
                     /locus_tag="PLK64_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 1,2-dioxygenase"
                     /protein_id="HPV79502.1"
                     /translation="MGEVVGAGILAHVPTIMLPEHERRALNHGEDFTIGPALTQLRHE
                     VFETLDYDTVVVLDSHWFTTVEFVVTAQDRRSGLFTAEELPRGMCRIPYDWRGDPELA
                     FAVERVAPQHSAWITANDDHFLPMYYPPINLWTFLGAGLDKRWVTVSVAQTGEADDFL
                     RAGRAIGQAIAETDRKVLLIGSGALSHTFWKLRYLRDHEAAGPEHIFTPEAYAADLER
                     IAWFTQGDHRRVLDTMPDFMRFRPEAMFAHYLMLAGALGESDLTAPGRQYGEYENSLG
                     TGQVHLWFDRPEGGFPRPQASAPEPNYDRQRAAMLAVGAPGAGPQPVS"
     gene            5022..5882
                     /locus_tag="PLK64_06880"
     CDS             5022..5882
                     /locus_tag="PLK64_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPV79503.1"
                     /translation="MTTREFRRILLDGNAVDVRVDGADLVAGDGRRVAIADAIHLPPV
                     TPSKIVCVHLNYISRVKELMIGLPPAPTYFHKPVSALNAHDAAVVRPAGCKWLNYEGE
                     IAIIIGRTARNVSPEEAGDYIRGYSVGNDYGLHDFRDTDSGSMLRVKGADTLCPIGPG
                     VVEGWDFRGKQIQTRVNGVVKQDGNTDEMEWNMHYLVADIARTITLQPGDILMSGTPA
                     ISRTVYPGDVVEVEVEGLGTLRNHIVEGPTPIRTDVGAQPTETEEVLSTAQGGDWEFR
                     GIRKPLPPAH"
     gene            5882..7405
                     /locus_tag="PLK64_06885"
     CDS             5882..7405
                     /locus_tag="PLK64_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPV79504.1"
                     /translation="MSAPDVVGDTDWVALAADVVAGRTPGMPSGLVIDGAPRPTRSGA
                     TRAIVSPRDGATLTVLPWAAADDVDDAVASARNAFRDGPWARLHPRERGELMVEFARV
                     IDAHRSELALLVALEMGKPVRAAYEIELRALLRTVRYYGELADKGSGEITASSASELS
                     LVTREPAGVVAAVVPWNFPLTLAGWKFAPALAAGCTVVLKTAEQSPLSMLRVAELAIE
                     AGLPPGVLNVLSGDGIEVGAALGRHPDVDILTFTGSTDVGRHFLRYSADSNLKRVYLE
                     LGGKSPNIVFPDAPDLDAAADTAAWAIFFNSGEMCTAGSRLILHRDIADRVLDRVVAT
                     AADWFPADPLLADTRMGPVVDARSLTRLRSTLDSGLASGARLVHGGRQVLADTGGSYL
                     EPTVVTDLDPANVLVRDELFGPILSVLVVDSEEEAIRVAGDTPYGLAAAVWTGDLGRA
                     HRVSKALRAGTVWVNCYEEGDLNVPFGGVKLSGFGRDKSRHAIDEYTDLKTTWIAHG"
     gene            7398..8171
                     /locus_tag="PLK64_06890"
     CDS             7398..8171
                     /locus_tag="PLK64_06890"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HPV79505.1"
                     /translation="MADDSTAVAGVPGPLAGRHTRPRIAVPGRFSTNASALRSRALVG
                     SRSLLDGVYAAGGDPLVVLPSAPGGEVTEAEVSDRLSWAEGILLPGGGDLAAHWSGQP
                     DHEALYDVDVEQDAFDLTIARWALRIGLPVLAICRGSQVVNVALGGTLVQHMDERGGP
                     IANHRNHVHHVSTTPGSRMASVVGAAVEVSCYHHQCIDTLADGLVATAHSEEGVVEGV
                     EIPSGAGWFLGTQWHPEDTWTERPEQLAVFEAFVAACRL"
     gene            complement(8205..8720)
                     /locus_tag="PLK64_06895"
     CDS             complement(8205..8720)
                     /locus_tag="PLK64_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPV79506.1"
                     /translation="MPDLHTLEETERAVARHTSHLSLDVASMYAVSSIFRAGNAVRDT
                     MTRDVLRPHDLTWTGFLVLWILWIWESRESHEVASAVGITKATLTGVVHTLTTRGWVD
                     REVPAMDRRRVRLTLTSSGLALMDELFPRFNAEESRAVSALTPAEIATLTNLLRKVVR
                     TYEVEDESPSA"
     gene            complement(8833..9624)
                     /locus_tag="PLK64_06900"
     CDS             complement(8833..9624)
                     /locus_tag="PLK64_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HPV79507.1"
                     /translation="MNGFFYPRTATGQAALIPPPPWHYSGDLLTVEYRTDPERVRELL
                     PAPLELAPEDPGAVAFIWADWQSCRASKEELLDPVLSQYKEAFVVVRCAFQGVTYSRC
                     VYIWVDKDFAILRGMHQGYPKKLGSIHQTRPHPYGPAPRVAVGATFGATVAAADRRLA
                     EAVLTLTRESETNGFVNSHPMAHHRVYPSIERGAQTDAYSELISSGSASFEAGPAFAG
                     EVELRLFEAPTEEVARLEVEEIIGGYYRQVGVAWDGGSTLAKLIP"
     gene            9749..>10493
                     /locus_tag="PLK64_06905"
     CDS             9749..>10493
                     /locus_tag="PLK64_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HPV79508.1"
                     /translation="MGHFDTSGLQGAADSLTEQGIDVLRLGYSDLIGTDRGRDILVNR
                     MPRTVSSGVAFCRSIYGTTPRGETVDIEGGVQAGLPDIVAFPDLSTLLPIPWEPGVAH
                     CIADLYNPDGTPSPESPRAVLKRVRERFAELGMTPVCGPELEFYILEANEKSHNGWIR
                     YGEASGNVYVSGLRGDPENVMLTTLRKLGAYGIEVITANHEYSSGQFEINLWHSEALD
                     AADRAHRFKNAIKEIARSLGKLATFMAKPF"
BASE COUNT         1690 a         3644 c         3594 g         1565 t
ORIGIN      
        1 gccgtcaggc caaccccgcg aagtgcagga agccctcgac aaggcgctcg gcccggcccg
       61 cggcgtctgc ctcgagccgg cgggtcaggg cgaggaggtc ctcccggtcg atcccctccg
      121 ccgtgagcac ggcgtcgccg ccgttgtcga gccatccgag gagcatgtcg acggtgacct
      181 ccggatggaa ctgcaggccg aggttgcgtc gtagcgagaa tgcttgcgac gcagcctcat
      241 tgcgcgccag ctcgacgcca tcgggcggga cgacccaccg atcggcgtgc cactggaacc
      301 acggccccgg cggcacgatc tcgggttcat ccgtggagat atcgacccag ccgatctcgc
      361 cctggggggc tcggctcacc gacccgccat gagtctgcgc gagcagttgt ccgccgaagc
      421 agatccccag gaccggtatg ccgcccgcgt cggcttcccg cagcaggtcc tgttccggga
      481 gcacccacga ccccaccaac tcgatctcgt agaccgacca cggcgccccc atcgccagca
      541 ccgcatcgac accgctcagc ggcggaaacc ggccggggac gtcgggggcg tcgaagcgtt
      601 ccggcgggac gacgaggtga tagacgcact cccaccccct cgcttcgaac gcggcggcga
      661 ccggtcccag cggcgacacg tggtcgtgct ggatgacgag aagccgccgc acctcccgcc
      721 gcacgtcaag gctcactcgc ccacgtcgat gaggccgacc tccgagggac ggtgtcgacc
      781 catctgggcg tattgctccg gagaggtgct gcgcagccgg agggcgagcg ccagaccgac
      841 gagcgcagca acgacgacga gggacggcag cgcccaagcg agcgggctgt ccgggctgcc
      901 gatgaggacc ccgaactgcg acgcggcata caccgacacg gagcccaggg ccacggtgcc
      961 gatgatgggg gcgaccgtcg tgcgccaggg gttggtgtcc aggcccttgt ggcgggcgaa
     1021 gaacgcggtg accgctgccg aggacagggc catgaggacg aggatggcca aggtccccaa
     1081 ctgggtgagc caggtgaaca gggccagcac ggggtcctgg cctgacacga tgaagacgac
     1141 gatgaccacg agcgcgagga ccgactggct caccgagccc ttgtgcgggc tgaggtggat
     1201 cttgtgggtg tgtccgagcg gtgtcgggag caaaccttca cggccgaggg cgaagaagta
     1261 gcgggcgacg gcgttgtgga aggccagcag ggcggcgaag acgctggtgg cgaagaggac
     1321 ggtcatgatc gtcgtcggca gttcgccgag gtagatcccg ctgatctcga acaggaaggc
     1381 ggtcgggtcc ggggtgaggc cgccgatgtg gtcgacgagg ccgtcggctc cctgcgcgtt
     1441 gaccatgagc cacgtcgtca ctgtgtagaa cagaccgatc gtcaacacgg cggcatacgt
     1501 cgcgcggggc acggtgcgct tggggtcgcg ggcctcttcg gagtagatcg tcgtggcctc
     1561 gaagccgatg aaggatgccg agttgaagag caacgcgatg gccagcgagc ccgacatgag
     1621 cgagtccatc gagaacgagg acatgctcag cccggctccg ccgccctggc cgcccttgat
     1681 gatgacggcg aggtccagca cgacgacgat gaggaactcg gccgcgacaa gcacgccgag
     1741 caccttgacc gacaggtcga cctggcggta gccgaggaaa ccgatgatga ccccggcgat
     1801 gaggacatag ctccaccagg gccaggccac gccgaactga gcctccatga agcccgaggc
     1861 tgcggcgccg aacagcccgt agaggccgat ctgcatggcg ttgtagccga gcagtgcgat
     1921 gagcgcggcg atcccgccgg ctcgctgccc caaccctcgg gcgacgtagc tgtagaaggc
     1981 accggtgctc gtgtggtgtc gagccatcgc cgtgtagccg accgcgaaga tcagcaggag
     2041 cacggcgacg atgacgtagg caaaggggat gccggcgccg ttgcccagga gcatggcgac
     2101 gggggcacct ccggccatcg cggtcagcgg ggccgcggcg gagatgacga agaaagcgat
     2161 gccgagcacc ccgaggctgc ccttgcgcag ctggtgctct ccttgcggag tgttcgttga
     2221 gctcatgagt tggctctctc tccccaccgg gcgagccgtc gcatcccgca cgacgcccgc
     2281 atgatttgta tggggccgaa cgataatgtg acctggagca cttgacaagg gtgtgaaagt
     2341 tcataacgtt tggcacctaa cgagtaggcc cgcaccccga gaaggtccca tgacccagca
     2401 cgccccggat caccccccgg cagctccgct ccaggacggc ttcatcgcgc aggtcgaggg
     2461 ggtgcgagtc gacactcgcc actggatcgg cggccgccgc gtggctctgg ccaccgccgg
     2521 agcggagggt gccgtcgcga ccttcacgac catctccccg atcgatgagt cgccgctggc
     2581 cgaggtctgc ctcggcgggt ccgcagaggt cgacgccgcc gtcgcagcag ctcaggcggc
     2641 cttccctggg tgggcggcgc tgtcggtgca agagcgctcg gagatcctcc gtcgggtcgc
     2701 cgatggcatc caagcccgga tcgaggacct gtcccgggtc gagactcgcg acaacggctc
     2761 gctcatccgc agccaccgcc gcggcgtgat gccgcgcgtc gccatgaact tccgcttctt
     2821 cgccgactac gcgcaggatc acctcggcca ccccgacctc accgtcccgg ggcgtggcca
     2881 ccgcgagcgg atcagctacg acccggccgg cgtcgtcgcg atcatcaccc cgtggaacgc
     2941 ccccctcatg ctcgccacat ggcgcatcgg cccggctctg gcagcgggga actgcgtcgt
     3001 cctcaagccg ccggagtggg ccccgctcac cgcgtccctg ctcgccgaga tcacccacga
     3061 cgcaggtatg ccgccgggtg tcttcaacgt cgtacagggc accggggcat cggctggggc
     3121 cccgctgacc cggcatccgg ggatcaaccg gctgtgcttc accggaagcg tccccaccgc
     3181 cggcgtcatc gcggcagcgg cagccacgaa catcgtcccg ctgtccttcg aacttggcgg
     3241 caagagcccg ttggtcgtct ttgacgacgc cgacctggat ctcgccgtgg agatcgccgt
     3301 cgagcagttc gacaacgccg gccaggtctg cctcaaggcg gcccggatga tcgtccacga
     3361 gtcgatagcg caggagttca cccggcgtgt ggtggacggc gccgcgaagc tcacccaggg
     3421 cgacccccgc gacgaggcca ccgacgtgtc ggcgctcatc acgcgcaagc acttcgaggt
     3481 cgtctccgga ttcgtcgagc gagccatcgc agacggcgcc cggccgttgc tcggcggcgg
     3541 tccgaacact gacctgggag ggctctactt ccggcccacc gtgctcgtcg acgcgcccga
     3601 ggacagcgag atcctcaccg aggaggtctt cggaccggtg gtgacgatcc agaccttcgc
     3661 caccgaggag gaggcggtcg cacgggccaa cgacagccgg ttcgggctgg ccgcgaccct
     3721 cgtcaccggc gaccgcgacc gtgccgagcg agtgtcggcc cggttggacg caggaaccgt
     3781 gtgggtcaac tgcttcttcg tccgcgacct cgaggcaccc ttcggcggca acggcaagtc
     3841 cggcatcgga cgtgagggcg gcgtctggtc gttcgacttc tacaccgaca tcaagaacag
     3901 cgtctttgct ccccacggct ggaaggacaa ctgacatggg cgaggtcgtc ggcgcgggaa
     3961 tcctcgcgca cgtccccacg atcatgcttc ccgagcacga gcggcgggcc ctcaaccatg
     4021 gcgaggactt caccatcggc ccggccctga cccagttgcg ccacgaggtg ttcgagaccc
     4081 tcgactacga cacggtcgtg gtcctggact cgcactggtt caccacggtc gagttcgttg
     4141 tcacggcgca ggatcgacgc tccgggctgt tcactgccga ggagttgccg cgcggcatgt
     4201 gccggatccc ctacgactgg cgcggtgacc ccgagctcgc cttcgccgtc gagcgggttg
     4261 cgccgcagca ctcggcatgg atcaccgcca acgacgatca cttcctgccg atgtactacc
     4321 cgccgatcaa cctgtggacc ttcctggggg ccggtttgga caagcgatgg gtcaccgtct
     4381 cagtcgccca gacgggcgag gccgacgact tcctgcgcgc cggccgggcc atcggtcagg
     4441 ccatcgccga gaccgaccgc aaggtcctgc tcattggcag cggcgctctc tcgcacacct
     4501 tttggaaact gcgctacctg cgcgaccacg aggccgccgg acccgagcac atcttcaccc
     4561 ccgaggcgta tgccgcggac ctggaacgca tcgcgtggtt cacgcagggc gatcaccggc
     4621 gggtcctgga caccatgccg gacttcatgc gcttccggcc cgaggcgatg ttcgcccact
     4681 acctcatgtt ggccggggcg ctcggggagt ctgacctcac tgcgccgggt cgtcagtacg
     4741 gcgagtatga gaactcgctc gggaccggcc aggtccacct gtggttcgac cggcccgagg
     4801 gcgggttccc ccggccgcag gcgagtgccc ccgaacccaa ctacgaccgc caacgagcag
     4861 ccatgctggc ggtcggagct cccggggcag gcccccagcc ggtctcctga ccgaccgccg
     4921 cctgccggtc ctccggcagt ccgccgacag agccaccgac agtccgcacg gcatacccct
     4981 tggcagtcca cacggcatac cggccactcc taggagagca gatgacaacc cgcgagttcc
     5041 gacgaatcct gttggacggc aacgctgtcg acgtgcgcgt cgacggcgcc gacctcgtcg
     5101 ccggggacgg ccgtcgggtc gccatcgccg acgcgatcca cctgccgccg gtgacgccga
     5161 gcaagatcgt ctgcgtccac ctcaactaca tctcccgggt caaggagctc atgatcgggc
     5221 tgcctccggc cccgacctac ttccacaagc cggtgtcggc cctcaacgcg cacgacgctg
     5281 ccgtcgtccg tccggccggg tgcaagtggc tcaactacga gggcgagatc gccatcatca
     5341 tcggccgcac ggcgcgcaac gtcagccccg aggaggcggg ggactacatc cgcggctact
     5401 cggtcggcaa cgactacggc ctgcacgact tccgcgacac cgactcgggg tcgatgctgc
     5461 gggtcaaggg cgccgacacg ctgtgcccca tcgggccggg tgtcgtcgag ggctgggact
     5521 tccgcggcaa gcagatccag acccgggtca acggcgtcgt caagcaggac ggcaacaccg
     5581 acgagatgga gtggaacatg cactacctcg tcgccgacat cgcccggacg atcaccctcc
     5641 agccgggcga catcctcatg tccgggaccc cggccatctc gcgcacggtc taccccggcg
     5701 atgtcgtcga ggtcgaggtc gaaggcctgg gcaccctgcg caaccacatc gtggaagggc
     5761 cgaccccgat ccgcaccgac gtgggcgcgc agccgaccga gaccgaggag gtcctctcca
     5821 cggcccaggg cggtgactgg gagttccgcg ggatccgcaa gccgctgccc ccggcccact
     5881 gatgagcgcg cccgacgtgg tcggcgacac ggactgggtg gcgctcgccg ccgacgtggt
     5941 cgccggacgc actcccggta tgccgtccgg cctggtcatc gacggggccc cccgaccgac
     6001 ccgatcgggt gccacccggg cgatcgtgtc gccgagggac ggggccacgc tcaccgtgtt
     6061 gccgtgggcc gcggctgatg acgtcgacga cgcggtggcc tcggcccgga acgccttccg
     6121 agacgggccc tgggcgcggc tacacccccg cgagcgcggc gaactcatgg tcgaattcgc
     6181 ccgagtcatc gacgctcacc gcagtgagct ggccctgctc gtcgccctgg agatgggcaa
     6241 accggtgcga gcggcatacg agatcgagct gcgggcgctc ctgcggacgg tgcggtatta
     6301 cggcgaactc gccgacaagg ggtccggcga gatcaccgcg tcatcggcga gcgaactgag
     6361 tctggtcacc cgcgagcccg cgggcgtggt cgcggccgtg gtgccgtgga acttcccgct
     6421 caccctggcc gggtggaagt tcgcccccgc gctggccgcc gggtgcacgg tcgtgctcaa
     6481 gacggccgag cagtcgccgc tgtcgatgct gcgggtcgcg gagctcgcca tcgaggcggg
     6541 tctgccgccc ggcgtgctca acgtgctgtc cggcgacggc atcgaggtcg gtgcggccct
     6601 cggtcggcat cccgatgtcg acatcctcac cttcaccgga tcgaccgacg tgggtcggca
     6661 cttcctgcgc tactcggccg actccaacct caaacgggtc tacctcgagc ttggcggaaa
     6721 gagccccaac atcgtcttcc cggacgcccc cgacctcgac gccgcagccg acaccgccgc
     6781 gtgggcgatc ttcttcaaca gcggcgagat gtgcacggcc gggtcaaggc tcatcctgca
     6841 tcgggacatc gccgaccggg tcctggaccg ggtcgtcgcg acggccgcag actggttccc
     6901 ggccgacccg ctcctggcgg acacccggat ggggcccgtc gtcgatgcgc ggtcgttgac
     6961 ccggctgcgc tcgaccctgg actcgggtct ggcctccggc gcgcggctcg tccacggtgg
     7021 tcggcaggtg ctggccgaca cgggtggctc ctatctcgag ccgaccgtcg tgaccgacct
     7081 cgacccggcc aacgtcctgg tccgtgacga gcttttcggg ccgatcctct cggtcctcgt
     7141 cgtcgactcc gaggaggagg cgatccgcgt cgccggagac accccctacg gcctggccgc
     7201 cgccgtctgg acgggcgatc tcgggcgcgc acaccgggtc agcaaggcgt tgcgagccgg
     7261 caccgtctgg gtcaactgct atgaggaggg cgatctgaac gtccccttcg gcggggtcaa
     7321 actgtcaggc ttcgggcgtg acaagtcgcg gcacgcgatc gacgaataca ccgacctcaa
     7381 gacgacctgg atcgctcatg gctgacgatt ccaccgcggt cgctggggtg ccggggcccc
     7441 tggcggggcg gcatacccgt cctcggattg ccgtcccggg gcggttctcg accaacgcct
     7501 cggccctgcg ctcgcgcgct ctcgtcggct cgcggagcct ccttgacggg gtgtatgccg
     7561 ccggcggcga cccgctcgtg gtgctcccct ccgcgccggg cggggaggtg accgaggccg
     7621 aggtcagtga ccggctctcc tgggccgaag ggatcctgct gcccggcggc ggcgacctcg
     7681 ccgcgcactg gtcggggcag ccggaccatg aggccctcta cgacgtcgac gtcgagcagg
     7741 acgcctttga cctcacgatc gctcgctggg ccctgcggat cgggttgccc gtgctggcga
     7801 tctgccgagg ctcgcaggtc gtcaacgtgg cgctcggcgg aacgctggtg cagcacatgg
     7861 acgagcgggg cggaccaatc gccaaccacc gcaaccatgt ccatcacgtg tcgacgacgc
     7921 cgggctctcg gatggcctcc gtcgtgggtg ctgccgtcga ggtgtcctgc taccaccacc
     7981 agtgcatcga cacccttgcc gacggactcg tcgccacggc gcactcggag gagggagtag
     8041 tcgagggggt cgagatcccg tcaggggcgg gctggttcct cggcacccag tggcaccccg
     8101 aggacacctg gaccgagcgg cctgagcagt tggccgtctt cgaagcgttc gtcgccgcct
     8161 gccgcctcta ggggcccgct agggggggcc ggctaaggac ctgcctaggc cgacggggat
     8221 tcgtcctcga cctcgtaggt ccgcacgacc ttgcgcagca ggttggtgag cgtggcgatc
     8281 tctgccggtg tgagcgccga aacggccctg gattcctcag cattgaaccg ggggaacaac
     8341 tcatccatga gcgccagtcc gctgctggtg agggtcagcc gcacccggcg ccggtccatg
     8401 gcgggcacct cacggtcgac ccagccgcgc gtggtgaggg tgtgcacgac gcccgtcagt
     8461 gtcgctttgg tgatgccgac cgcagaggcc acctcgtggc tctcgcgcga ctcccagatc
     8521 cagaggatcc acaggacgag gaagccggtc caggtgaggt cgtgggggcg tagcacgtcg
     8581 cgcgtcatgg tgtcgcgaac cgcgttcccc gcgcggaaga tcgacgacac ggcatacatc
     8641 gaggcgacgt ccaacgacag gtgagaggta tgccgcgcaa cggcgcgctc ggtctcctcg
     8701 agggtgtgga ggtcgggcac gggagcaacc atatctaccc acctgaggcg ccccgaggga
     8761 cggtcgtcct gtcgtgagcc ggcctagtcg acggtccctg gccgaccatc cccgtcgacc
     8821 agggcgcgca cgtcagggga tgagcttcgc cagggtcgag ccgccgtccc acgcgacgcc
     8881 gacctggcgg tagtagccgc cgatgatctc ctcgacctcg agccgggcga cctcttccgt
     8941 gggggcttcg aagagccgca actccacctc gccggcgaag gccggcccgg cctcgaacga
     9001 cgccgatcct gaggagatca gctcggagta ggcgtcggtc tgggcgccgc gctcgatcga
     9061 ggggtagacc cggtggtggg ccatggggtg ggagttgacg aagccgttgg tctccgactc
     9121 gcgcgtgagg gtgagcacgg cctcggccag gcgccggtca gcggccgcca cggtcgcgcc
     9181 gaacgtggca ccgacggcga cccggggggc tgggccgtag gggtgcggtc gggtctggtg
     9241 gatcgacccg agcttcttcg ggtagccctg gtgcatcccg cgtaggatcg cgaagtcctt
     9301 gtcgacccag atgtagacgc agcgcgaata cgtgacgccc tgaaaggcgc accggacgac
     9361 gacgaaggcc tccttgtatt ggctgaggac tgggtcgagc agctcctcct tcgaggcccg
     9421 gcaggactgc cagtcggccc agatgaaggc gaccgcgccc gggtcttcgg gagccaactc
     9481 gagcggggcc ggaagtagct cgcggacgcg ttcggggtcg gtgcggtatt cgaccgtgag
     9541 caggtcgccg ctgtagtgcc acgggggtgg cgggatcagc gcggcctggc ctgtcgccgt
     9601 gcgggggtag aagaagccgt tcacggtccg tcctcgtcta ggcgatcgtt tggggcctca
     9661 cgatagcggt gttaggtgga catgtcaccc cttgccagac gacccagagg tcagtacgtt
     9721 cgcctccaaa cgatctagga ggtgtggcat gggtcacttc gacacgagcg ggctccaagg
     9781 cgcggccgac tcgctgaccg agcagggcat cgacgttctc cgactgggat attccgacct
     9841 catcggaacc gaccgggggc gcgacatcct ggtcaatcgc atgccgcgga cggtgagcag
     9901 cggggtggcc ttctgtcggt ccatctacgg caccacgccg cgcggcgaga cggtcgacat
     9961 cgagggcggc gtccaggccg gcctccccga catcgtcgcc ttccccgacc tcagcacgct
    10021 gcttcccatc ccgtgggagc ccggtgttgc tcactgcatc gccgacctct acaaccccga
    10081 cgggacaccc tcgccggaga gcccgcgggc cgtgctcaag cgggtccgcg agcggttcgc
    10141 ggagctcggc atgacccccg tctgcggccc cgagctggag ttctacatcc tcgaggccaa
    10201 cgagaagtcc cacaacgggt ggattcgtta tggcgaggcc agcggcaacg tctacgtgtc
    10261 cgggctgcgt ggagacccgg agaacgtcat gctgacgacg ctgcgcaagc tcggtgccta
    10321 tggcatcgaa gtcatcacgg ccaaccacga atactcctcg ggccagttcg agatcaacct
    10381 ctggcattcc gaggctctcg acgccgccga ccgcgcccac cgattcaaga acgccatcaa
    10441 ggagatcgcc cgcagcctgg gcaagctcgc gaccttcatg gccaagccct tca
//
LOCUS       DAOMZY010000092        10424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_6027_length_10424_cov_5.591667, whole genome shotgun sequence.
ACCESSION   DAOMZY010000092 DAOMZY010000000
VERSION     DAOMZY010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 10424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10424
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6027_length_10424_cov_5.591667"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="PLK64_06910"
     CDS             <1..1157
                     /locus_tag="PLK64_06910"
                     /inference="COORDINATES: protein motif:HMM:NF026115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyketide synthase dehydratase
                     domain-containing protein"
                     /protein_id="HPV79509.1"
                     /translation="AIALDWTAWGGIGMATRGSIPKIMEMAGVQMLPPDAGVAWIRRE
                     VTSTAYSGEVVVAGVLGMMAQEYHETGGADPAALAGAGPHGPMVGTASASVHNGLVVT
                     TTLDPKEQAFLYDHRIDGTPVLPGVMGMESFAEAAAMLAPEGYRVGSVEDVAFLAPVK
                     FFRDEPRTLTITAKAVPAPEGTDLLVRCTLTAERALPGQATPVTTTHFTGTVRLTTEP
                     VAEEVEKVKTKVDGTPLSNDNVYAFYFHGPAYQVVGEAWRKGDGSMTRMASPIPANHV
                     PDEAPLAIAPRLAELCFQTAGLWEAGTAHQMALPTRVGRLRVLRDPATVDGPLYALAR
                     PAGESRFDCAVVDGRGSVVMRMDGYESIVLPAPIPESVMGALTATFGTDS"
     gene            1282..6681
                     /locus_tag="PLK64_06915"
     CDS             1282..6681
                     /locus_tag="PLK64_06915"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPV79510.1"
                     /translation="MNDISRLAIIDRGEPVVRVLAAVGNLNRRGDVPSITSIVVTEQS
                     SERAWFAREADELITPPPSPDEVDLSTHIEPGQVVAQVLGAKADTAWIGHTPCLDRAE
                     LVAQLEAAGVRVVGPTAQTIRDLADPARLAALGRESGLSPVPDHLTPADWRRIEIDVL
                     ADSAGTVWTLGLRDASIRRGPTPVLAEMPAPGVSDTTAGAMSHAVRRLVRAAAYRGAG
                     VVELALAHDGESFWLVGVDTLARTEHALVEEITGASFIGLRLRLAAGDALPATPPTPN
                     GHAVEVRLLAHDPGREYAASGGALEMLSLPVGTGVRVDSSIREGDLVDGRVEPLIATV
                     TAWGRDREEALDRAHGALERTTVVLEIGMTNRTADLAVLERVAAAEQPFDAGWYDRTL
                     AAGGFTSVADPLAVVAAAVEAYESDSTMVNAAFFASAARGRPERPERVGSLVQLSYRG
                     HDYRLRVDRTGPRSYRVRGNATVDLIVDRLGDFERRIICNGHKHRLVAVEQGADFRLD
                     LDGVGHTVSREDGVVVRTGWPALVSSLLVEPGQDVSAGQPVAVLESMKMVTTVTAPFD
                     GTVTSLAVSNNTQIERGAPLMRIKAASHQHLDLGPGDGVGAEPESAVDFSALVTSDGN
                     PEGKDATWVHAHLGDYLLGYDLDPESFKGLLKRQATLGATLPANDPELVGAENALLDL
                     FSDLGALYRPRTEAENPDQMADDNTQEYFLSYLQWLDADQAGLPERYRKRLGAALARY
                     GVAGLSRTKALEAATVWLYRAFARIPEIAPAIVAILNRRLTHHDELADMATLDARARL
                     DRLVRATEGRQQNVSDLARDIIFHFFEEPEMLAATAQMQDQVRADLAALRDLPSGPDR
                     EARIEALVAAPHPVRTDLLDAWLQTDADAAGTAYRDVVLEVYARRFYRIRDLQDFGTR
                     SVGAFRIAHADYEHAGMPVRLVNSYLPLEQLPPFADALRGYLCDVPAGREVVVDLVVW
                     QNGKLPDADALVEKVHGIIEHCDFGHALHRLDLTVTGQVGDASGRSTAHMTFRQGADG
                     AFTEDRLYRNLHPMLAKRLDLWRLSNFTLERLPSTEDVYLFFGVAHENPKDRRLFAVA
                     EVRDLVAVRDDQTGAQTYPRLGRIGLQALAAMRTALSHYPPRERPTANRLVLNVRPVW
                     EIPADEWPALAANYLPLAKGAGLEKVVMHLRTPVPAEDGGTTLVEKVMTIDGIGRTGT
                     TIRFGDPGANPIRPLTRYAQKVLTAERFGTPYPYEIIHMLTPAEGEPSPFPRGHFQEL
                     DLDADGETLIPVDRPPAGNTAHLVVGLLTTYTDLVPEGMTRLAMLSDPTQGLGNLAEP
                     ECRLINASLALAAERKIPVEWYACSSGALIAMDSGTENMDFIALTLRRIIEFTQAGHE
                     INIVVTGINVGGQPYWNAEATMLMHTKGILIMTPASTMVLTGKQALDFSGAVSAEDNF
                     GIGGYDRVMGLNGQGQYWAPSFQDACRLLLEHYNYTYVVPGERFPRRGLTSDPVERDV
                     RESAHEQVPGTRFTTVAEVFDFATNPDRKQPFDMRSVMRAVSDTDCEPLERWKHWQDG
                     ENSIVWDVAVGGIPVCMIGLSSRSLPRKGFVPADGPPAWTSGTLFPQASRKTARAINA
                     TTGNRPLVVMANLSGFDGSPESMRKWQLEYGAEIGRAITNFNGPIVFIVVSRYHGGAF
                     VVFSKALTESMEIAAIEGSYASVIGGAPAAATVFARDVKIRTDKDPRVVEALAAAAAA
                     KGPEAGRLRAKAAAVAAAVRSEKLGEVADEFDSIHTIERALAMGSVDRIITAEELRPY
                     IIGALERGMAKFTTS"
     gene            6681..7613
                     /locus_tag="PLK64_06920"
     CDS             6681..7613
                     /locus_tag="PLK64_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019158211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HPV79511.1"
                     /translation="MVLTTDDVLAARERTKGYLRRTPLLPPSRTEAGVWLKCEFLQHT
                     GVFKARGAFNLLLAARERGELDPQAGVVIASGGNAGLATAYAAGVLRVPATVFVPSIA
                     SAVKVARLRDYGADVHVVGTEYSHAASAAADFARVRGALASHAYDLPEVVAGAGTLAL
                     EVLEDEPGIDTIVVAVGGGGLYAGIAAAVDGRARIVTVEPARAPSLYAALAAGRPVDV
                     EVSGVAADSLGARRVGEIAFAMAAASTPISLLVDDEEILRARDLMWRDYRIAAEAGAA
                     TAYAALTGGAYRPEPSERVALIVCGANTDPATLR"
     gene            7671..10202
                     /locus_tag="PLK64_06925"
     CDS             7671..10202
                     /locus_tag="PLK64_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018762238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPV79512.1"
                     /translation="MPHTASDGMVHVRGAREHNLKNVDVDIPRDALVAFTGVSGSGKS
                     SLAFGTLYAEAQRRYLESVSPYARRLFHQMAVPEVDEIDGLPPAVALQQQRGSSTTRS
                     SVGSVTTLSNLLRMLYSRAGDYPPGQPLLYAESFSPNTPQGACPTCHGLGRVYEVTEK
                     SLVPDDSLTIRERAIAAWPTAWGGQNLRDILVSMGIDVDRPWRDLPQDQREWILFTDE
                     QPVVPVYAGLTPAETRRARKHKEEPSYQGTFSSARRHVFTSFANTESPKMKKRALHFM
                     LSDTCPLCHGKRLRPESLSVTFAGLDLADLSRLPLEELARTIEPYADGSSPRLAEVAA
                     EHPEQALVISRITGDLRARLSTLLQLGLGYLNLERSTPTLSPGELQRLRLATQVRSNL
                     FGVVYVLDEPSAGMHPADTEALLTALDRLKAAGNSLFVVEHDLDVIRRTDWIVDVGPA
                     AGEHGGTILYSGPPEGLRDVASSQTRHFLFESDEARAGRAPREPEDWLRLRGVTRNNV
                     HDLEIAFPKGVFTTVTGVSGSGKSSVVSQALVELVAQALGSDAPAEDAEGDELEIAPV
                     LTLGGEIVSGMEGIKRLVRVDQKPIGRTPRSNLATYTGVFDHIRRLFAATPAARERHY
                     GAGRFSFNVPKGRCPNCNGEGFVMVELLFLPSVYAPCPVCKGTRYNPKTLEITYRGKS
                     IADVLGMTVDGACDFFDDEPAVRRSLTVLCDVGLQYLRLGQPATEFSGGEAQRIKLAT
                     ELQRPQRGDSLYILDEPTTGLHPADVVRLTRQLDSLVASGNTVIAVEHDMSMVAASDW
                     VIDIGPGAGAKGGQIVASGPPREVAQGPGRTAPYLASYLGSPSAG"
     gene            complement(10215..>10424)
                     /locus_tag="PLK64_06930"
     CDS             complement(10215..>10424)
                     /locus_tag="PLK64_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79513.1"
                     /translation="DYVRPDGWSPLVVHAGTSERFPGWWRRDGVFLLTLASREVTDPP
                     ALLAGGDDLATGEPTHSWMANPLAD"
BASE COUNT         1548 a         3785 c         3505 g         1586 t
ORIGIN      
        1 gtgccatcgc gttggactgg acggcgtggg gcggtatcgg catggccacc cgtggctcca
       61 tccccaagat catggagatg gccggcgtgc agatgctgcc gccggacgcc ggtgtcgcgt
      121 ggatccggcg tgaggtgacg agcacggcat acagcggcga agtggttgtt gccggcgtcc
      181 tcgggatgat ggcgcaggaa taccacgaga ccggcggcgc agacccggcc gccctcgcgg
      241 gtgccggccc ccacggcccg atggttggga ccgcctcggc cagcgtccac aacggtctgg
      301 tcgtcaccac gacgctcgac cccaaggagc aggcgttcct ctacgaccac cggatcgacg
      361 gcaccccggt gctgccgggc gtcatgggga tggagtcgtt cgccgaggcg gcggccatgc
      421 tggcccctga gggctaccgc gtcgggtcgg tcgaggacgt cgcgttcctc gccccggtga
      481 agttcttccg ggacgagccg cgcactctga ccatcacggc gaaggccgtc ccggcacccg
      541 agggcaccga tctgctggtg cggtgcaccc tcaccgccga gcgggccctg cctggccagg
      601 ccacccccgt cacgaccacc cacttcaccg gcaccgtccg gctgacgacc gagccggtcg
      661 ccgaagaggt ggagaaggtc aagaccaagg tcgacggcac tccgctgagc aacgacaacg
      721 tctatgcctt ctacttccac ggcccggcct accaggtggt cggcgaggcg tggcgtaagg
      781 gtgacgggtc gatgacccgg atggcgtcgc cgatcccggc gaaccacgtc cccgacgagg
      841 caccgctggc catcgcccct cggctggccg agctgtgctt ccagaccgcc ggactgtggg
      901 aggcaggcac cgcccaccag atggcgctgc ccacccgggt cggccgactg cgggtcctgc
      961 gggacccggc gacggtggac ggaccgctct atgccttggc acgcccggcg ggcgagagta
     1021 ggttcgactg cgccgtggtc gacggtcgtg ggtcagtggt catgcggatg gacggttacg
     1081 agagcatcgt gctcccggca ccgattccgg agtccgtgat gggcgcgctc acggcgacct
     1141 ttggcaccga tagctgaaca cgcgttacgg tcgggtcgcg ctcgcgaggc atcgagcctc
     1201 gtgggcgcgg cccgttcgcc catcctgcgc gccatgtggc gcgtcccttt cgagcgacaa
     1261 cttcattgat cggagacctg cgtgaacgac atctcccgac tggcgatcat cgaccgaggt
     1321 gagcccgtcg tgcgggtgct cgctgccgtc ggcaacctga accgtcgagg agacgttccg
     1381 tcgatcacat ccatcgtcgt caccgagcag agcagcgaac gtgcctggtt cgcccgcgag
     1441 gccgacgagc tcatcacccc gccgccgtca ccagacgagg tggacctcag cacccacatt
     1501 gagcctggcc aggtcgtcgc gcaggtcctc ggggcgaagg ctgacaccgc ctggatcggc
     1561 cacacgccgt gcctggaccg ggccgagttg gtcgctcaac tcgaagccgc cggagtgcgg
     1621 gtcgtcggcc ccaccgcgca gaccattcgc gacctcgccg accccgcccg gctcgccgcc
     1681 ctcgggcgcg agtcggggct ctcgcccgtc cccgaccacc tgacgcccgc cgactggcgc
     1741 cgcatcgaga tcgacgtgct ggccgactcc gccggaacgg tgtggaccct cggccttcgc
     1801 gacgcgagca tccgccgcgg ccccaccccg gtgctcgccg agatgcccgc ccccggcgtg
     1861 tccgacacga ccgccggcgc gatgtcccac gccgtgcgcc ggttggtgcg tgccgcggca
     1921 taccgtggcg cgggcgtggt cgagttggcc ctcgcccacg acggcgagtc gttctggctg
     1981 gtcggcgtcg acacgctggc gcgcaccgag cacgccctcg tcgaggagat caccggggcg
     2041 agcttcatcg ggttgcggct gcgacttgct gcgggcgacg cgctgcccgc cacgccgccc
     2101 acgcccaacg ggcacgcggt cgaggtgcgg ctgctcgcgc acgacccggg ccgggagtat
     2161 gccgcgtccg gcggagcgct ggagatgctc tcgctgccgg tggggaccgg cgtgcgcgtc
     2221 gactcgagca tccgcgaggg tgacctcgtc gacggccggg tcgagccgct catcgcgacc
     2281 gtcaccgcct gggggcgcga ccgcgaggaa gccctcgacc gcgcccacgg cgccctcgaa
     2341 cgcaccacgg tcgtcctcga gatcggcatg accaaccgga cggccgacct cgccgtcctg
     2401 gagcgcgtcg ccgcagccga gcagcctttc gatgccgggt ggtacgaccg gactctcgcg
     2461 gccggcggct tcacctccgt cgccgacccg ctggccgtcg tcgctgcggc cgtcgaggcc
     2521 tacgagtccg acagcaccat ggtcaacgcc gccttcttcg cgagcgcggc tcgcgggcgc
     2581 cccgaacgcc ccgagcgggt cgggtcgctg gtgcaactga gctaccgcgg gcacgactat
     2641 cggctgcggg tcgaccgcac cggtccgcgc tcctatcgtg tgcgcggcaa cgcgaccgtc
     2701 gacctcatcg tcgaccggtt gggtgacttc gagcgccgga tcatctgcaa cggccacaag
     2761 caccgcctcg tcgccgtcga gcagggtgcc gacttccgcc tcgacttgga cggcgtgggg
     2821 cacaccgtca gccgcgaaga cggcgtcgtc gtgcgcaccg gctggccggc cctcgtctcg
     2881 tcgttgctcg tcgagcccgg ccaggacgtc tcggccggtc agcccgtggc tgtcctcgag
     2941 tcgatgaaga tggtgaccac ggtgaccgcg cccttcgacg gcacggtgac ctcgttggcg
     3001 gtctccaaca acacccagat cgagcgcggc gcgccgctca tgcggatcaa ggccgccagc
     3061 caccagcacc tggacctcgg gccaggcgac ggcgtcggcg ccgagcccga gtcggccgtc
     3121 gacttctcgg ccctcgtcac ctccgacggc aacccggagg ggaaggacgc gacctgggtc
     3181 cacgcccacc tcggggacta cctgctgggc tatgacctcg accccgagag cttcaaggga
     3241 ctgctcaagc ggcaggcgac cctcggcgcc accctgcccg ccaacgaccc tgagctcgtc
     3301 ggtgccgaga acgccctgct cgacctgttc agcgacctcg gggccctcta ccggccgcgc
     3361 accgaggccg agaaccccga ccagatggcc gatgacaaca cgcaggagta cttcctctcc
     3421 tacctgcagt ggctcgatgc cgaccaggcc gggctgcctg agcgctaccg caagcgcctc
     3481 ggcgctgcgc tggcccgcta cggcgtcgcc ggactgtctc ggaccaaggc cctcgaagcg
     3541 gccaccgtct ggctctaccg cgccttcgcc cggatcccgg agatcgctcc ggccatcgtg
     3601 gcgatcctca accggcgcct cacccaccac gacgagttgg ccgacatggc caccctcgac
     3661 gcgcgggccc gcctcgaccg gctcgtgcgc gccaccgagg ggcgccagca gaacgtctcc
     3721 gacctggccc gtgacatcat cttccacttc ttcgaagagc ccgagatgct cgcggcgacg
     3781 gcccagatgc aggaccaggt gcgggccgat ctcgccgcgc tgcgcgacct gccgtccggg
     3841 ccggatcgcg aagcacgcat cgaggcactc gtcgcggccc cccacccggt ccgcaccgac
     3901 ctgctcgacg cgtggttgca gaccgacgcg gacgcggcgg gcacggcata ccgggatgtc
     3961 gttcttgagg tctacgcgcg gcgcttctat cgcatccgcg acctgcagga cttcggcacc
     4021 cgctccgtcg gcgccttccg catcgcgcac gcggactacg agcacgccgg tatgccggtc
     4081 cgcctcgtca actcctacct ccccctggag cagttgccac cgttcgcaga cgccctgcgc
     4141 ggctacctgt gcgacgtgcc agcgggccgg gaggtcgtcg tcgacctcgt cgtctggcag
     4201 aacggcaagc tccctgacgc cgacgctctc gtcgagaagg tccacggcat catcgagcac
     4261 tgcgacttcg gccacgccct gcatcgcctc gacctcaccg tgaccggcca ggtcggcgat
     4321 gcgagcgggc ggtcgacggc acacatgacc ttccgtcagg gcgccgacgg tgccttcacc
     4381 gaggaccggc tctatcgcaa cctgcacccg atgctcgcca agcggctgga cctctggcgc
     4441 ttgtccaact tcaccctgga gcggctgccg tccaccgaag acgtctacct cttcttcggc
     4501 gtcgctcacg agaaccccaa ggaccggcgc ctgttcgccg tggctgaggt ccgtgacctc
     4561 gtcgccgttc gcgacgacca gaccggagcc cagacctacc cgcggctcgg ccggatcggg
     4621 ctgcaggccc tggccgcgat gcggaccgcg ttgtcgcact acccgccccg cgagcgcccg
     4681 acggccaacc ggttggtgct caacgtgcga ccggtctggg agatcccggc cgacgagtgg
     4741 cccgcgttgg cggccaacta cctgccgctg gccaagggtg ccgggctgga gaaggtcgtc
     4801 atgcacctgc ggaccccggt gcctgccgag gacggcggca ccaccctggt cgagaaggtc
     4861 atgaccatcg acggcatcgg ccgcacgggc acgaccatcc gcttcggcga cccgggggcc
     4921 aacccgattc ggccgctgac gaggtatgcc cagaaggtgc tcaccgcgga gcgcttcggc
     4981 acgccctacc cctacgagat catccacatg ctcacgccgg ccgagggcga gccgtcgccg
     5041 ttcccccgcg gtcacttcca ggagctcgac ctcgacgccg acggcgagac gctcatcccg
     5101 gtggaccggc caccggcagg gaacaccgca cacctggtcg tgggcttgct gacgacctac
     5161 accgacctgg tgcccgaggg catgacccgg ttggcgatgt tgtccgaccc gacgcagggc
     5221 ttgggcaacc tcgccgagcc cgagtgccgc ctgatcaacg cctcgctcgc gctcgcggcc
     5281 gagcggaaga tccccgtcga gtggtatgcc tgctcgtccg gcgccctcat cgcgatggac
     5341 tcgggcaccg agaacatgga cttcatcgcg ctcacgctgc gacggatcat tgagttcacc
     5401 caggccggtc acgagatcaa catcgtcgtc accgggatca acgtcggcgg ccagccctac
     5461 tggaacgccg aagcgacgat gctcatgcac accaagggca tcctcatcat gaccccggcc
     5521 agcacgatgg tcctcaccgg caagcaggcc ctggacttct ccggggcggt ctcggccgag
     5581 gacaacttcg gcatcggtgg ctacgaccgg gtcatggggc tcaacggcca gggccagtac
     5641 tgggcgccga gtttccagga cgcctgccgt ctgctgctgg agcactacaa ctacacctat
     5701 gtcgtgcccg gggagcgttt cccgcggcgc ggcctgacga gcgacccggt cgagcgggac
     5761 gtccgcgagt ccgcgcacga gcaggtgccg ggcacccgct tcacgacggt cgccgaggtc
     5821 ttcgacttcg cgaccaaccc cgaccgcaaa cagcccttcg acatgcgctc ggtcatgcgc
     5881 gcggtgtccg acaccgactg cgagccgttg gagcggtgga agcactggca ggacggcgag
     5941 aactccatcg tctgggacgt ggccgtgggc ggcataccgg tctgcatgat cggtctgtcc
     6001 tcccgctcgc tgccgcgtaa gggcttcgtc cccgccgacg ggccgcccgc ctggacctcg
     6061 ggcacgttgt tcccgcaggc gtcccgcaag acggcgcgtg ccatcaacgc gaccacgggc
     6121 aaccgcccgc tggtcgtcat ggccaacctg tccggcttcg acggctcgcc ggagtcgatg
     6181 cgcaagtggc agttggaata cggcgccgag atcggtcggg ccatcaccaa cttcaacggc
     6241 ccgatcgtct tcatcgtcgt ctcccgctac cacggcggcg ccttcgtcgt gttctccaag
     6301 gccctcaccg agtcgatgga gatcgccgcg atcgaggggt cctacgcctc ggtcatcggc
     6361 ggtgctcccg cggccgcgac ggtgttcgcc cgcgacgtca agatccgcac cgacaaggac
     6421 ccgcgggtcg tcgaagcctt ggctgccgcc gcggcggcca agggtccgga ggccggtcgg
     6481 ttgcgggcca aggctgctgc ggttgcagcg gccgtgcgct cagagaagct cggtgaggtg
     6541 gccgacgagt tcgactcgat ccacaccatc gagcgggccc tggcgatggg gtcggtcgac
     6601 cggatcatca cggcggagga gttgcggccc tacatcatcg gcgcactcga gcgcgggatg
     6661 gccaagttca cgacgagctg atggtcctca ccacggacga cgttctcgcc gcccgcgagc
     6721 gaacgaaggg ttacctccgg cgcaccccgc tcctgcctcc gtcacgcacg gaggcagggg
     6781 tgtggctcaa gtgcgagttc ctccagcaca cgggtgtgtt caaggcgcgt ggagccttca
     6841 acctgctgct ggctgcgcgc gagcggggcg agctcgaccc acaggcgggt gtcgtcatcg
     6901 cctcgggcgg caacgccggt ttggcgacgg cgtatgccgc gggcgtcctc cgcgtcccgg
     6961 cgaccgtctt cgtcccgtcc atcgcgtcag ccgtcaaagt ggcgcggttg cgcgactacg
     7021 gggctgacgt ccatgtcgtg gggacggagt actcccacgc agcgtcggcc gcggctgact
     7081 ttgcccgcgt gcgcggcgct ctggcgtcgc acgcctatga cctgccagag gtggttgctg
     7141 gggcggggac gcttgccctg gaggtgctcg aggacgagcc gggcatcgac accatcgtcg
     7201 tggcggtcgg cggcggcgga ctgtatgccg ggatcgctgc ggccgtggac ggtcgcgcgc
     7261 ggatcgtgac cgtcgagccg gctcgagcgc ccagtctgta tgccgccctg gctgccgggc
     7321 ggccggtcga cgtcgaggtc tcgggggtgg cggccgactc gctgggtgcc cggcgggtgg
     7381 gtgagatcgc gttcgccatg gcagccgcgt cgacgcccat cagcctcctg gtcgacgacg
     7441 aggagatcct gcgggctcgc gacctgatgt ggcgggacta ccgcatcgcc gcggaagccg
     7501 gcgccgcgac ggcatacgcc gcgttgactg gcggtgctta ccgcccggag ccgagcgagc
     7561 gcgtcgccct catcgtctgc ggagccaaca ccgacccggc cacgctgcgc tgaccgcacc
     7621 ggtccggcgt tcaccctgct tcgggccggt ggcaggaata cagtcgtcgc atgccccaca
     7681 cggcctccga cgggatggtg cacgttcgtg gagcccgtga gcacaacctg aagaacgtcg
     7741 acgtcgacat cccccgggat gcgctggtgg cgttcacagg cgtctccggc tccgggaagt
     7801 cctcgctcgc gttcggcacg ttgtatgccg aggcgcagcg ccgctacctc gaatccgtct
     7861 ccccctatgc ccgccgcctc ttccaccaga tggcggtgcc ggaggtggac gagatcgacg
     7921 gcctgccacc ggctgttgcc ctgcaacagc aacgcggctc gtccacgacc cgctcgtcgg
     7981 tcggcagtgt caccacgctg tcgaacctcc tgcgcatgtt gtattcgcga gcaggcgact
     8041 atccgccggg tcagccactg ctgtatgccg agtcgttctc accgaacacc ccgcaggggg
     8101 cctgcccgac gtgccacggc ctggggcggg tctacgaggt gaccgagaag tccctcgtcc
     8161 cggatgacag cctgaccatc cgcgagcggg ccatcgccgc ctggccgacc gcctggggtg
     8221 gccagaacct gcgcgacatc ctcgtctcga tggggatcga cgtggatcga ccgtggcgcg
     8281 acctgccgca agatcaacgc gagtggatcc tgttcacgga cgagcagccc gtggtgcccg
     8341 tgtatgccgg cctgacacct gcggagacac gtcgcgcgcg caagcacaag gaggagccga
     8401 gttaccaggg gacgttctcc agcgcccgcc ggcacgtctt cacctcgttc gcgaacaccg
     8461 agagcccgaa gatgaagaag cgggcgctgc acttcatgct cagcgacacg tgcccgttgt
     8521 gccacggcaa gcggctgcgt ccggaatccc tgtccgtgac gttcgccggc ctggacctgg
     8581 ccgacctgtc gcggctcccc ctggaggaac tcgcccggac catcgagccc tacgctgacg
     8641 gctcctcgcc tcggttggcg gaggttgcgg ccgagcatcc cgagcaggcg ctggtcatca
     8701 gccggatcac cggcgatctg cgggcccgcc tgagcaccct gctgcaactc gggctcggct
     8761 atctcaacct cgagcgcagc acgcccaccc tctcccccgg ggagttgcaa cgtctgcggc
     8821 tggcgaccca ggtccgctcc aacctgttcg gcgtcgtcta tgtcctggac gagccctcgg
     8881 ccgggatgca cccagccgac accgaagctc tgctgactgc gctcgatcgc ctcaaggctg
     8941 cgggcaactc gctctttgtc gtcgagcacg atctggacgt catccgtcgc accgactgga
     9001 tcgtcgacgt cggaccggcg gcgggcgagc acggcggcac catcctctac agcggtccgc
     9061 ccgaaggcct acgggacgtg gccagttcgc agacccggca cttcctcttc gagagcgacg
     9121 aggcccgggc ggggcgtgcc cctcgcgagc ccgaggattg gctgcgcctg cgaggggtga
     9181 cgcgcaacaa cgtccacgac ctggagattg ccttccccaa gggcgtcttc acgacggtga
     9241 ccggcgtgtc cggttcgggc aagtccagcg tcgtgagcca ggcgctcgtg gagttggtcg
     9301 cgcaggccct gggctccgac gcccctgccg aggatgccga aggcgacgaa ttggagatcg
     9361 ctcccgtgct cacgctgggc ggcgagatcg tttccggcat ggagggcatc aagcggctgg
     9421 tccgcgtcga tcagaaaccc attgggcgga cgccgcgctc caacctggcg acctataccg
     9481 gcgtgtttga ccacattcgt cgcctgttcg ccgcgacgcc ggccgcgcgg gaacgccact
     9541 acggcgctgg ccggttctcc ttcaacgtcc ccaagggtcg atgcccgaac tgcaacggcg
     9601 agggcttcgt catggtcgag ttgctgttcc tgccgagcgt ctatgcgccg tgcccggtgt
     9661 gcaaggggac ccgttacaac cccaagaccc tggagatcac ctaccgcggc aagtcaattg
     9721 ccgatgtcct cggcatgacg gtcgatggcg cgtgcgactt cttcgatgac gagccggcag
     9781 tgcgacggtc gctgacggtg ttgtgcgacg tcggcctgca atacctgcgg cttgggcaac
     9841 ccgcgaccga gttctccggc ggggaggccc aacggatcaa gctcgccacc gagttgcagc
     9901 ggccgcagcg cggcgactcc ctctacatcc tcgatgaacc gaccacgggg ctgcacccgg
     9961 cagacgtggt gcggctgacc cgtcagctcg attccctggt ggcctcaggc aacaccgtca
    10021 tcgcggtgga gcatgacatg agcatggtgg ccgcgagcga ctgggtgatc gacatcggtc
    10081 cgggcgccgg cgcaaagggt ggtcagatcg ttgcgtccgg tcccccgcga gaggtggcgc
    10141 agggcccggg ccgcaccgcg ccctacctgg cgtcctatct gggttcccca tccgcggggt
    10201 gacccgacga cacctcagtc ggcgagcggg ttggccatcc acgagtgggt cggctcccca
    10261 gtggccaggt cgtccccgcc cgcgagcaga gcaggcgggt cggtcacctc gcgactggcc
    10321 agcgtgagca gaaacacccc gtcgcgccgc caccagccgg ggaaccgctc gctggtgccg
    10381 gcgtgtacga ccagggggct ccagccatcg gggcgcacgt agtc
//
LOCUS       DAOMZY010000093        10404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_6042_length_10404_cov_6.551841, whole genome shotgun sequence.
ACCESSION   DAOMZY010000093 DAOMZY010000000
VERSION     DAOMZY010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 10404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10404
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6042_length_10404_cov_6.551841"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PLK64_06935"
     CDS             <1..308
                     /locus_tag="PLK64_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HPV79514.1"
                     /translation="LAAAPPQSRLVQVLLADPEPLLFHAEILLRDGVPVGDVRSGSYG
                     WTLGGAVGLAMVTNPDGPASADWLSAGTWEVDIAGQRYAARVSLRPLYDPTSARVNA"
     gene            494..889
                     /locus_tag="PLK64_06940"
     CDS             494..889
                     /locus_tag="PLK64_06940"
                     /inference="COORDINATES: protein motif:HMM:NF025379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HPV79515.1"
                     /translation="MPTITETLKTKLDEFELERRIDELTVATEKVVKRAIEQAGDLAH
                     DNRDKVTTLLDKAGSAIDQRTDGKYHDTVGKVRAQVVSGVDKLATKRPTHHTPFDTDN
                     EATEANEAPTSNETPQDAPTTWSAHTDPV"
     gene            1003..1491
                     /locus_tag="PLK64_06945"
     CDS             1003..1491
                     /locus_tag="PLK64_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007269336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPV79516.1"
                     /translation="MTSPRDVVTVQRLIPAPASAIFALLADPSRHPEIDGSGTVLHPS
                     SPGSRHLGLGDTFGMSMKLGVPYSTRNVVIEFEEDRRIAWQTLAAAPIDKVLTGRVWR
                     YELQPVDGGTIVRETWDISREATLSRPIVRRLMGAQTRINMARTLERIESAVAGTGLG
                     SA"
     gene            1512..4631
                     /locus_tag="PLK64_06950"
     CDS             1512..4631
                     /locus_tag="PLK64_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HPV79517.1"
                     /translation="MHDQGSIILARVRRVLAERIRPAETTVVTPLDAAAWEVTEPDGI
                     PGQGEPVPAQVALAADFTPTAVGMPWGPPWGTTWFRFRASVPPDLRGAPLEAVIDLGW
                     TSGSVGGQAEGLVFDKGGRIIKGIHPRNAWVPVQPNDKGKVRFYVEAAANPEVLRSRS
                     FAPTSVGRKQTADPTPLYRLARADLVEVHHEVRELIADVVTLLGLAEVLPETATRAWT
                     VLRALDEAMDALDLADIVGTAAAARAVLAPALAVRAAEGTHEISAIGHAHIDSAWLWP
                     VRETRRKVARTVADVLHLAESRPGVVFALPAAQHGAWLEEDQPDLFARLQAAVAAGAV
                     VPVGGMWVEPDGNLLAGESVCRQLTFGARWFAERLGHRCDEVWLPDSFGYSAALPQLA
                     RLAGMRWMLTQKISWNEVDRFPHHSFLWEGIDGTRIFTHFPPADTYSSELTSVDLTRA
                     ASAFADKGRARRSLLPFGYGDGGGGPTREMFAQADRVADLDGSPRVRVESPAAFFERA
                     EAEHRDPSVWVGELYLERHRGTFTSQARTKAGNRRCEGLLREAELWWTSVALRGLSDY
                     PAQALDRLWRQVLLGQFHDILPGSSIGWVHDEIERTHAEVEAELEDLITRALALLCNE
                     GEAPQDADADGPTEWVSFSADPLAGDGVALGGTAYLGDSEPSAPAVTVVATGTAAVLD
                     NGRLQVTIDARGLVTSVLDLAADRELIPPGTVGNLLQCHPDLPNAWDAWDLDPFYRNT
                     VVDLTEVEELGVQTLDDGSAEVRVVRPLGTRGSQATQTLSLAPGAEHLDIEVEVDWRE
                     RDTILKLAWPLDVHTAEARFETQFGYVTRPIHENTTWDRYRFEVPALRWVHVGEPGYG
                     IAIANARTYGWDVSRSARAGGATFTTVRASLLRGSQFPDPDADLGTHRFGFRLVPGAD
                     VGGAVAAGYAVAHPPRVVAGRAGRHTEPVVRVEGAVVETVKPAEDGSGALVVRLYEHL
                     GRRAAATLALAPDVPLDLAQAHEVDLHEQPRDTIDQPSAVRGLDEFGSLRLALGPFQI
                     LTVRISTKS"
     gene            4679..5947
                     /locus_tag="PLK64_06955"
     CDS             4679..5947
                     /locus_tag="PLK64_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV79518.1"
                     /translation="MSRAPDEAVRLRTPWQSRPFRVLTIGMVISTFGSSITPVAIAFA
                     VLSLGGTATQLGLVVAAYALAEVATVLVGGVLGDRLPRAAVVQGAAMASAVTQGVLGL
                     SLVKGWSSIGLVALLSALTGIVGSLSIPSASAMTQQMVPGYLLQRAISIRRLLGNSAS
                     VIGFGAGGMLVAAVGPGWAILVDAATFLVASILLASVSLPSPAAEPGRSVLADALVGA
                     REVMRHTWLWVLIAVALVYHLFYASAQGVLGPIVVQMGYGEAAWGWALAVLMIGFMAG
                     GLVTLRWMPRHALYIGTAFLTLTACFPAALAANLNLGLVLTGAFLHGFGLELFSVAWD
                     LSIQENVAPDKLARVYGFDVLGSFIARPVGLALTGPLAEAVGYADWLWVVTGVVLAAT
                     LLALTVPAVRTLERQLPARTNDPDVQPTGG"
     gene            5957..6691
                     /locus_tag="PLK64_06960"
     CDS             5957..6691
                     /locus_tag="PLK64_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPV79519.1"
                     /translation="MLTDQPAHRVVATVSDTPVGDIDVLTWSPADAHIAEPLPMLFAH
                     DGPELDVFAELTALIGSCVASGRLPRMRVTLLAPGDRNPRYAASAAYAAALADHVVPL
                     QLRAHPSEHPPVLVGPSLGGLAALHAEWTHPGTFGGLLMLSGSFFTPELDPQEKGFEF
                     WEQVVGFVDRVHTSPAPSRPEVALGWGTQEENQFNNALMAQRLGELGLPVQTATVDNG
                     HNFTCWRDLLDLLLPDLLTRTWMPRT"
     gene            6695..7441
                     /locus_tag="PLK64_06965"
     CDS             6695..7441
                     /locus_tag="PLK64_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPV79520.1"
                     /translation="MDTARVELSAAGFDRPGTVLRYGHWGRPVLVFPCEAGRAWDFAD
                     RGMLAAVTPLVDAGRVKLYCVDSFDEATWSNRGLSIEDRARGHLAYEGWITGPVLDWV
                     RHDSPGAGDPVTTGVSMGAFHAVQFALRRADLFPQALAMSGSYDPSWWHAWGEPGDAA
                     YATNPSWYVPDLEGGHLDWLRSRLDLVLVVGRGPFETEPTRSLPSTRLLAGMLQDKGI
                     RAELDEWGEDVAHDWPWWQQQLAHHLPRFL"
     gene            7603..8850
                     /locus_tag="PLK64_06970"
     CDS             7603..8850
                     /locus_tag="PLK64_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79521.1"
                     /translation="MAHVHPIGLLLGAEEDWPHAFEAILRRVGPVVTTDGVTHTFTSE
                     RLTIEPFDLTDPVRYELVIDRLAHWYYHPREWLKKAALVNGTYLLNSPFTFQSMEKHS
                     AYCAMLRLGLKVPRTVLVPYKNPVDNDRWAPTSAKYNLPFDLSAIAADLGYPLYMKPF
                     DGGGWRGVSRIENEDELHSAYNESGEMLMHLQATVPHEKFARALSIGPETMVMDFRAE
                     KPMHSRYAVSHDFLSPTAGERAVAISRVVNAFFGWEFNSAEMLVQGDEVYPIDYANAC
                     PDVAVTSLHYYFPWAIKALVRWSVYCLVTGRPAKVDLQTERYFAIADDPSLDDDGKLA
                     AYLKIADEHFETERYWEWCATHLPHLDEQVLDWVTSTDFDRMLKDTVRATYPEHEATE
                     FLDHFHGLVDLWVIDEGARMRAD"
     gene            9153..9509
                     /locus_tag="PLK64_06975"
     CDS             9153..9509
                     /locus_tag="PLK64_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79522.1"
                     /translation="MTVTHDETRASVAKTRRARRQLDDAASYLEVAAFAALMGGTADD
                     LTRMTGCTEAITTLAGLGARGEVDGFGELAAGEDPIGAAVAALQGICADAFVDERHAV
                     VVGALADSLARRFLTR"
     gene            complement(9534..10241)
                     /locus_tag="PLK64_06980"
     CDS             complement(9534..10241)
                     /locus_tag="PLK64_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HPV79523.1"
                     /translation="MNTSAARSSTRRSTLTRERVLQAAVDLADDAGLEAVSMRRLGQE
                     LGVEAMSLYNHVNNKDDLLDGMVEMAMVEVNRSAREVRDAAPSDADWQGVLRAQVLAS
                     RQHMLRHRWLPVALENRAGMSVGVARYFDGVLGTLVAGGFSNDLGHHALHALGSRALG
                     FAQELFTPGGDEQDAASQAAMASMAPELPHLMAMLAEVAHDDPTSTLGWCDDQSEFEF
                     GLDVLLDGLERRRLRRP"
BASE COUNT         1507 a         3752 c         3504 g         1641 t
ORIGIN      
        1 ccctggccgc cgcccccccg cagagccgac tcgtccaggt acttctcgcc gaccccgagc
       61 cactgctctt ccacgccgag atcctgctgc gtgacggggt gccggtcggc gacgtccgct
      121 ccggctccta cggctggacg ctcggcggcg ccgtcggcct ggcgatggtc accaaccccg
      181 acggcccggc gagcgcggac tggctctcgg ccggcacctg ggaggtcgac atcgcgggcc
      241 agaggtatgc cgcccgcgtc tcgttgcgcc cgctctatga cccgacgagc gcccgggtca
      301 acgcctgacc gagcggcgac gcctgtccca cgcaacgcct cggcgccgct tccggccggg
      361 ttccgatggc cagggggagg ccagggtgaa ccctgagcgc tccgagggaa caccgcccca
      421 gcagggcacg ttgtgctgga cggctccgga agttcccggc gccgcacacc acgcaccgca
      481 ggggagaccc ccaatgccca ccatcaccga gaccctcaag accaagctcg acgagttcga
      541 gcttgagcgc cgcatcgacg agctcaccgt cgccaccgag aaggtcgtca agcgcgccat
      601 cgagcaggcc ggcgacctcg cacacgacaa ccgcgacaag gtcaccacgc tcctggacaa
      661 ggccggctcg gcgatcgacc agcgcaccga cggcaagtac cacgacaccg tcggcaaggt
      721 ccgcgcccag gtggtgtccg gcgtcgacaa gctcgccacc aagcgcccga cccaccacac
      781 cccgttcgac accgacaacg aggcgaccga ggccaacgag gcgcccacgt ccaacgagac
      841 cccgcaggac gcaccgacca cctggtcggc gcacaccgac ccggtctgat cccctgccgc
      901 ccgtcgcggc gctggtttag gtgtggtcga acgtgggccc tgccccccgt tcgaccacac
      961 tcatgttcgg cccccgtccg ggccgcgata gcgtcatcga ccatgaccag cccccgtgat
     1021 gtcgtcaccg tccaacgcct catcccggca ccggcgtcgg cgatcttcgc cctgctggcc
     1081 gacccgtccc gccaccccga gatcgacggc tccggcaccg tgctgcaccc atcgagcccc
     1141 ggctcccgac acctgggcct gggtgacacc ttcggcatgt cgatgaagct gggcgtcccc
     1201 tactccaccc gcaacgtcgt catcgagttc gaggaggacc gccggatcgc ctggcagacc
     1261 ctcgcggccg cccccatcga caaggtgctc accggccggg tctggcgcta cgagctccag
     1321 cccgtcgacg gcggcaccat cgtgcgcgag acgtgggaca tctcccgcga ggcgaccctg
     1381 tcgcgaccga tcgtccggcg actcatgggc gcccagaccc ggatcaacat ggcccgcacg
     1441 ttggagcgga tcgaaagcgc ggtcgccggg acaggcctag gctcggcctg acgccgagtt
     1501 gaggaggcca ggtgcacgat caaggctcca tcatcctggc ccgggtgcgc cgagtcctcg
     1561 ccgagcggat ccgcccggcc gagaccacgg tggtcacccc gctcgatgcc gcagcctggg
     1621 aggtgaccga gccggacggc ataccggggc aaggggagcc cgtccccgct caggtggccc
     1681 tcgccgccga cttcaccccc accgcagtgg gtatgccgtg gggtcccccc tgggggacca
     1741 cctggttccg cttccgcgcg agcgtgccgc cggacctgcg cggcgccccc cttgaggcgg
     1801 tcatcgacct cggctggacg agcgggtcgg tcggtgggca ggccgagggc ctggtgttcg
     1861 acaagggcgg ccggatcatc aagggcatcc acccccggaa cgcgtgggtt ccggtgcagc
     1921 ccaacgacaa gggcaaggtg cgcttctacg tcgaggccgc cgccaacccc gaggtgctgc
     1981 ggtcccgctc cttcgcgccc acgagcgtcg ggcgcaagca gaccgccgac ccgaccccgc
     2041 tctatcgcct ggcccgagcc gacctggtcg aggtgcacca cgaggtccgc gagctcatcg
     2101 ctgacgtggt gacgctgctc ggcctggccg aggtgctgcc cgagaccgct acccgcgcct
     2161 ggacggtcct gcgcgccctc gacgaggcga tggatgccct cgacctggcc gacatcgtcg
     2221 gcaccgctgc ggccgcccgc gccgtcttgg cgccggcgct cgccgtgcgg gccgccgagg
     2281 gcacccacga gatcagcgcc atcgggcacg cccacatcga ctcggcctgg ctctggccgg
     2341 tccgtgagac ccgacgcaag gtcgcccgga ccgtcgccga cgttctccac ctggccgagt
     2401 cccgtcccgg cgtcgtcttc gcgctgcccg ccgcccagca cggagcgtgg ctggaggagg
     2461 accagcccga tctcttcgcg cggctgcagg cagccgtggc cgcgggtgcc gtcgtcccgg
     2521 tcggggggat gtgggtcgag ccggacggca acctgctcgc cggcgagtcg gtatgccgcc
     2581 agctcacctt cggcgcgcga tggttcgccg aacgcctggg tcaccgttgc gacgaggtgt
     2641 ggttacccga ctcgttcggc tattcggcgg ccctgccgca gctcgctcgg ctggccggaa
     2701 tgcgctggat gctcactcag aagatctcgt ggaacgaggt cgaccgcttc ccgcaccact
     2761 cgttcctctg ggagggcatt gacgggacgc gaatcttcac gcatttccct cccgccgata
     2821 cctattcgtc cgagttaacc tcggttgatc tcactcgagc ggccagcgcg ttcgccgaca
     2881 agggccgggc gcgccgatcc ctgctgccgt ttggttacgg cgatggtggc ggcggcccga
     2941 cccgagagat gttcgcccag gccgaccgcg tcgccgacct ggacggctcg ccccgggtgc
     3001 gggtcgagag cccggccgcg ttcttcgagc gggccgaggc cgaacaccgg gatccgtcgg
     3061 tgtgggtggg cgagctctac ctcgaacgtc accgtggcac cttcaccagt caggcccgca
     3121 ccaaggccgg caatcggcgc tgcgaagggc tgctgcgcga ggccgaactc tggtggacga
     3181 gcgtcgcgtt gcgaggtcta agcgactatc cggcgcaggc cctggatcgg ctctggcgtc
     3241 aggtgctgct cggtcagttc cacgacatcc tgcccgggtc gtcgatcggc tgggtccacg
     3301 acgagatcga gcggacccat gccgaggtcg aagccgagct cgaggacctc atcacccggg
     3361 cgctcgccct cctctgcaac gaaggcgagg caccccagga cgccgatgcc gacggcccga
     3421 ccgagtgggt gtcgttcagc gccgacccgc tggccggcga cggggtcgcg ttggggggca
     3481 cggcatacct gggcgacagc gagccgagtg ccccggcggt gacggtcgtc gccaccggga
     3541 ccgcggcggt cctggacaac ggccggttgc aggtcaccat cgacgcccgc gggctggtca
     3601 cctcggtgct cgatctcgcg gccgaccgcg agctcatccc gcccgggacc gtcggcaacc
     3661 tcttgcaatg ccaccccgac ctgcccaatg cctgggacgc ctgggacctc gacccgttct
     3721 accgcaacac cgtggtggac ctcaccgagg tcgaggagct cggcgtccag accctcgacg
     3781 acggcagcgc cgaggtccgc gtggtccgtc ccctgggcac gcgcggctcg caggcgaccc
     3841 agaccctctc cctcgcccct ggcgccgagc acctggacat cgaggtcgag gtcgattggc
     3901 gcgagcgcga cacgatcctc aaactcgcct ggccgttgga cgtccacacc gccgaggcgc
     3961 gcttcgagac ccagttcggg tacgtcacgc gccccatcca cgagaacacc acgtgggacc
     4021 gctatcgctt cgaggttccc gcgctgcggt gggtgcacgt cggcgagccc ggctacggca
     4081 tcgccatcgc caacgcccgc acctacggct gggacgtcag ccgcagcgcc cgcgccggtg
     4141 gcgcgacgtt cacgacggtg cgggcgagcc tgctgcgcgg ctcgcagttc cccgacccgg
     4201 acgccgacct cggcacccac cgcttcggtt tccgcctcgt ccccggcgcc gacgtcggtg
     4261 gtgccgtcgc cgcggggtat gccgtcgccc acccgccgcg cgtcgtcgcg ggccgggccg
     4321 ggcggcatac cgagcccgtc gtgcgcgtcg agggggcggt ggtcgagacg gtcaaacccg
     4381 ccgaggacgg cagtggcgcg ctggtcgtgc ggctctacga gcacctcggc cgccgtgccg
     4441 cagcgacctt ggccctcgcg ccggacgtgc ccctggacct cgcccaagcg cacgaggtcg
     4501 acctgcacga gcaaccccgg gacacgatcg accagccatc agccgtccga ggcctcgacg
     4561 agttcggcag cctgcgcttg gcgctggggc ctttccagat cctcaccgtg agaatttcga
     4621 cgaaatcctg aacattctgt gacattccat cgtccgctga ccctttacgg tggaacctat
     4681 gtcgcgcgcc cctgacgaag cggtgaggtt gcgcacgcca tggcagtcac gaccgttccg
     4741 ggtgctgacg atcggcatgg tgatcagcac cttcggaagc tcgatcaccc cggtcgccat
     4801 cgcgttcgcg gtgctctccc tcggcgggac ggcgacgcaa ctgggtttgg tcgtcgcggc
     4861 gtatgccctg gctgaggtcg ccaccgtcct tgtcggtggc gtgctcggtg accggctgcc
     4921 gagggcggcc gtcgtgcaag gtgccgcgat ggcgtcggcg gtgacccaag gagtgctcgg
     4981 gctgagcctg gtcaagggct ggtcgagcat cggcctcgtt gcgctcctca gtgccctcac
     5041 cgggatcgtc gggtcgctgt cgatccccag cgcctcggcg atgacccagc agatggtccc
     5101 cggatacctg ctgcagcgag cgatctcgat ccggcgactg ctcggcaact ccgcgagcgt
     5161 catcgggttc ggggccggag gcatgctcgt cgccgccgtc ggcccggggt gggcgatcct
     5221 cgtcgacgcg gcaaccttcc tcgtggccag catcctgctc gcctcggtgt cgctgccctc
     5281 gcccgccgcc gagcccggtc gctcggtgct ggccgatgcc ctcgtcggcg cccgggaggt
     5341 gatgcggcat acctggttgt gggtgctcat cgcggttgcc ctcgtttatc acctcttcta
     5401 cgccagcgcc caaggcgtcc tgggcccgat cgtcgtccag atgggctacg gcgaagcggc
     5461 ctggggctgg gctctggcgg tgctgatgat cggcttcatg gccggcgggt tggtcacctt
     5521 gcggtggatg ccccggcatg cgctctacat cgggaccgcg ttcctcacgc tcaccgcctg
     5581 cttcccggct gcgttggctg ccaacctcaa cctcgggctg gtcctcaccg gggccttcct
     5641 gcacggtttc ggcctggagt tgttcagcgt tgcctgggat ctgtcgatcc aggagaacgt
     5701 cgcccccgac aaactggcgc gggtctacgg cttcgacgtc ctcgggtcgt tcatcgcccg
     5761 gcccgtcggc ctggccctca ccgggccgct cgcagaggcg gtggggtacg ccgactggtt
     5821 gtgggttgtc acaggggtcg tcctcgcggc gaccctgctg gccctcactg tgcccgcggt
     5881 gcgaaccctc gagcgtcaac ttccagcccg gaccaacgac ccggacgtcc agcccaccgg
     5941 cggataacgt ctggggatgc tcaccgacca accggcgcac cgagtcgttg ccacggtgtc
     6001 cgacactccg gtcggcgaca tcgacgtgct cacctggtca ccggccgacg cgcatatcgc
     6061 cgaaccgctg ccgatgctct tcgcccacga cggcccggag ttggacgtgt tcgccgaact
     6121 caccgccctc atcggctcct gcgtcgcctc cggacggctg ccacggatgc gggtcacctt
     6181 gctcgccccg ggagaccgca atccccggta tgccgcgtcc gcggcatacg ccgctgccct
     6241 cgccgaccac gtcgtgccgc tgcagttgcg cgcccacccg agcgagcacc cgccggtgct
     6301 cgtcgggccg agcctcggcg ggcttgcggc gctgcatgcc gaatggaccc acccggggac
     6361 cttcgggggc ctgctcatgc tgtcggggtc gttcttcacg ccggagttgg acccccagga
     6421 gaaggggttc gagttctggg agcaggtcgt cgggttcgtc gatcgcgtgc acacctcgcc
     6481 ggctccgtcg cggccggagg tggccttggg gtggggcacc caggaggaga accagttcaa
     6541 caacgccctc atggcccagc ggctgggaga gttgggcctg cccgtgcaga cggcgaccgt
     6601 cgacaacggg cacaacttca cctgttggcg tgacctgctg gacctgttgc tgcccgatct
     6661 gttgacccgc acctggatgc cgaggacctg acgaatggac accgctcgcg tggagctttc
     6721 cgcagccggc ttcgaccggc ctgggacggt gctgcgttac gggcactggg ggcgaccggt
     6781 gctggtgttc ccctgtgagg ccggtcgggc gtgggacttc gccgaccgag ggatgctcgc
     6841 cgcagtcacg cccctcgtcg acgccggtcg ggtcaagctc tactgcgtcg attccttcga
     6901 cgaggcgacc tggtccaacc gagggttgtc gatcgaggac cgggcccgcg gacacctggc
     6961 ctacgaggga tggatcaccg ggccggtgct ggattgggtg cgccacgact cgccgggggc
     7021 cggcgacccg gtgacgaccg gcgtgagcat gggcgccttc cacgccgtcc agttcgccct
     7081 gcgccgcgcc gacctcttcc cgcaggccct ggcgatgtcg ggaagctacg acccctcgtg
     7141 gtggcacgcg tggggcgaac ccggcgacgc ggcatacgcg acgaatccgt cctggtatgt
     7201 cccggatctg gagggtgggc acctcgactg gttgcgctcg cgactggacc tcgtgctcgt
     7261 cgtcggtcgc gggccgttcg agaccgagcc gacccgttcg ctgccgtcga cccggctgct
     7321 ggcggggatg ttgcaggaca aggggattcg ggcggagctc gacgagtggg gcgaggacgt
     7381 cgcccacgac tggccgtggt ggcagcagca actggcccac cacctcccgc ggttcctttg
     7441 acggctgcgt gccggcttgg tgacggcccg cgggagaccg atgcgcggtt gcgagcaacc
     7501 gatcactggg cgtcgtgacc ttcttcctgg atgtgtgcgg gtgggcctgg catcctgtcg
     7561 tgatcggact ccccgtccga tgcgaagggg gtcatcgtgg acctggcaca cgttcatccg
     7621 atcgggcttc tgctcggcgc cgaggaggac tggccgcacg cgttcgaggc catcctgcgc
     7681 cgcgtcggac cagtggtgac cacggacggg gtgacccaca cgttcacgag cgagcggctg
     7741 accattgagc cgttcgacct caccgacccg gtccgctacg agttggtcat cgaccgactc
     7801 gcccactggt actaccaccc gcgcgagtgg ctcaagaagg ccgcgctcgt caacgggacc
     7861 tacctgctca acagcccgtt caccttccag tcgatggaga agcacagcgc ctattgcgcg
     7921 atgctccggc tcggcctcaa ggtgccgcgg accgtcctgg tgccctacaa gaaccccgtc
     7981 gacaacgacc ggtgggcccc gacctcggcg aagtacaacc tgcccttcga cctcagcgcg
     8041 atcgccgccg acctgggcta cccgctctac atgaagccgt tcgacggcgg cggctggcgc
     8101 ggggtgtcgc gcatcgagaa cgaggacgag ttgcactcgg cctacaacga gtccggcgag
     8161 atgctcatgc acctgcaggc gacggtcccg cacgagaagt tcgcgcgcgc cctctcgatc
     8221 ggccccgaga cgatggttat ggacttccgc gcggagaagc ccatgcacag caggtatgcc
     8281 gtgtcccacg acttcctctc gcccaccgcg ggcgagcggg ccgtcgccat cagccgggtc
     8341 gtcaacgcct tcttcggctg ggagttcaac tcggccgaga tgctcgtgca gggcgacgag
     8401 gtctacccca tcgactacgc caacgcctgc cccgatgtcg cggtgacctc cttgcactac
     8461 tacttcccct gggcgatcaa ggcgctggtg cgttggtcgg tctactgcct ggtcaccggt
     8521 cgcccggcga aggtcgacct gcagaccgag cggtatttcg cgattgccga cgatccgtcg
     8581 ctggacgacg acggcaagct cgcggcatac ctgaagatcg cggacgagca cttcgagacc
     8641 gagcgttact gggagtggtg cgcgacccat ctgccgcact tggacgagca ggtgctcgac
     8701 tgggtgacct cgaccgactt cgaccggatg ctcaaggaca ccgttcgggc gacctatccg
     8761 gagcacgagg cgactgagtt cctcgatcac ttccatggtc tggtcgacct ctgggtgatc
     8821 gacgagggcg ctcggatgcg ggcggactag ccagctgagc gactgacgat gcgagcgagg
     8881 cccgccctgc gggctccagc ccgagtcgcc cgccgcttgt atgagggtag tgacgcttgt
     8941 atgccgttag agcctcatgc aagcgtcact agcctcatac aagccaggcc gtcggcacgc
     9001 ggcgctcgct gaagcgaccc acgtccgcgt cggcccagag ggccggaagg tcgattcgta
     9061 ccaaattcgg cgtcataaaa ggcagttgcg gacgtctcct cttcgacgcg cgatctcgcg
     9121 cgcaggtcgg ggtggttcat ggaggaggcg aggtgaccgt cacgcacgac gagacacgag
     9181 cgtcggtggc caagacccga cgcgcgcggc gccaactgga cgacgccgcg agttatctgg
     9241 aagtggcggc gttcgccgcg ctgatgggcg ggaccgccga cgatctgacc cgaatgaccg
     9301 gctgcaccga ggcgatcacg accttggccg ggctcggcgc tcgcggcgag gtcgatggct
     9361 tcggtgaact ggccgcgggc gaggacccga ttggtgcggc cgtggctgcg ctgcagggga
     9421 tctgtgctga cgccttcgtc gatgagcggc atgcggtggt cgttggtgcc ctggccgaca
     9481 gcctggcccg gcgcttcctc acccgctgac cggttcggcc gctgacggag ggcttacgga
     9541 cgtcgcaggc gccggcgttc caacccgtcg agcaacacgt cgagcccgaa ctcgaactcg
     9601 ctctggtcgt cacaccaccc caaagtggac gtggggtcgt cgtgcgcgac ctcggcgagc
     9661 atcgccatca ggtgtggcag ctcgggcgcc atcgacgcca tggcggcctg cgacgcggca
     9721 tcctgctcgt cgccgcccgg ggtgaagagc tcctgggcga accccagcgc gcggctgccc
     9781 agagcgtgga gggcgtggtg gcccaggtca ttcgagaagc caccggccac cagggtcccc
     9841 agcacgccgt cgaagtaccg cgccaccccg acactcatgc cggcgcggtt ctcgagcgcg
     9901 acaggcagcc agcggtggcg gagcatgtgc tgacgggagg ccagcacctg ggcccggagc
     9961 accccctgcc aatcggcgtc tgacggggcg gcgtcgcgga cctcccgcgc ggatcggttg
    10021 acctcgacca tggccatctc gaccatcccg tcgaggaggt cgtccttgtt gttgacgtgg
    10081 ttgtagagcg acatcgcctc gacgccaagc tcctgcccaa gcctgcgcat cgagacggct
    10141 tcgagcccgg cgtcatcggc caggtccacg gccgcctgca ggacccgttc gcgggtcagc
    10201 gtggatctcc gggtcgagct ccgggcggcc gacgtgttca cccgctcatc atcgcgtggg
    10261 ttcgagccat ccgtcgccgc ctcccactct cggggtgtcc tgaccttgac ggcaggagcc
    10321 gtctgaggtt tacttacgct gtaaatatat acgccgtaag tagagcgctc gtccaggctc
    10381 acgacctcgg aggcaccatg accc
//
LOCUS       DAOMZY010000094        10285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_6131_length_10285_cov_5.853666, whole genome shotgun sequence.
ACCESSION   DAOMZY010000094 DAOMZY010000000
VERSION     DAOMZY010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 10285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10285
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6131_length_10285_cov_5.853666"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="PLK64_06985"
     CDS             <1..1087
                     /locus_tag="PLK64_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV79524.1"
                     /translation="SILGLPAAGWVVHRFGAARTVLAGVIASVLALATVGATTEILHS
                     RPLTMAALFFAGLGIGIWDMSMNLEGATVERAAGRAIMPRFHAAFSAGTVFSALVGSG
                     LTALGVGVLPHLAGTSLVVLLAALWATPRFLTTGAGAHGIPDAADSTGTEPSTSGLGM
                     RAAWTERRTLLIGLVVLVAAFTEGSANDWLAIAFVDGHHLPAWAGVLAFATFLASMTV
                     GRIAGTKLLDRYGRVRVLRATFLTAVLGSVLVVFGGPWLAFLGAALWGVGASLGFPVG
                     MSAGADDPARAAARVSVISTIGYGAFLGGPPLLGFLGDHIGVLRAMLVVGLAVLLAFA
                     ALPAVREPAPEEDFPQISPQVGFPDGS"
     gene            1128..1577
                     /locus_tag="PLK64_06990"
     CDS             1128..1577
                     /locus_tag="PLK64_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HPV79525.1"
                     /translation="MDADIASYLDKVKQRRSELGQSMAELQEVIDEPVGDAEHWRDVA
                     RAAAADVRRDLDEHIAVTEANGGLYDSLRAEAARLAGPLRHLQGEHPDLVALADELIA
                     VLDRPAAADRADAPELVKKLLRRLSHHRQRGADLVFEAYQVDVGGTG"
     gene            1723..2328
                     /locus_tag="PLK64_06995"
     CDS             1723..2328
                     /locus_tag="PLK64_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HPV79526.1"
                     /translation="MAGAPMPGSLDDHIYNRLLKERIIFLGSEVRDENANAICAQMLL
                     LAAEDPEKDIWLYINSPGGSVTAGMAIFDTMQWVPNDVATVAMGMAASMGQFLLSAGT
                     PGKRYATPHARVMMHQPSGGIGGTASDIKIQAEQMLHIKKQMAALIAEHTGQTLEQIE
                     RDSDRDRWFTAEEAKAYGFVDHVVERASSAAHRADNPVTGN"
     gene            2355..3002
                     /locus_tag="PLK64_07000"
     CDS             2355..3002
                     /locus_tag="PLK64_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HPV79527.1"
                     /translation="MTMNAGMAPMAPMGRYILPQFEERTSYGMKRLDPYTKMFEDRII
                     FLGVQVDDASADDIIAQLIVLESQDPDRDILMYINSPGGSFTAMTAIYDTMQYIRPDI
                     QTFVVGQAASAAAVLLGAGAPGKRYALPNARILIHQPALAGGDYGQASDIEIQANEVL
                     RMRTWLEETLALHTGRTAEQTARDIERDKILSSAEAKEYGLIDEVLLSRKGSAQK"
     gene            3192..4475
                     /gene="clpX"
                     /locus_tag="PLK64_07005"
     CDS             3192..4475
                     /gene="clpX"
                     /locus_tag="PLK64_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPV79528.1"
                     /translation="MARVGDGSDLLKCSFCGKSQKQVKKLIAGPGVYICDECIDLCNE
                     IIEEELAETSELGLDELPKPREIFDFLGTYVVGQDIAKKALAVAVYNHYKRIQAGEGM
                     GRKGEDAVDIAKSNILLIGPTGCGKTYLAQTLARMLNVPFAIADATALTEAGYVGEDV
                     ENILLKLIQAADYDVKKAETGIIYIDEVDKIARKSENPSITRDVSGEGVQQALLKILE
                     GTTASVPPQGGRKHPHQEFIQIDTTNVLFIVGGAFAGLEKLIEARSGKKGLGFGSQLH
                     LAKEEGWSYADVMPEDLMKFGLIPEFIGRLPVITTVTPLDRAALVAILSEPRNALIKQ
                     YQRMFEIDGVELEFTPEAVEAVAEQALLRGTGARGLRAIMEEVLQPVMFDVPSENDIA
                     RVVITREVVLDNVNPTIVRREAARVVKHAPRRQSA"
     gene            4548..5183
                     /locus_tag="PLK64_07010"
     CDS             4548..5183
                     /locus_tag="PLK64_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79529.1"
                     /translation="MDRDSQPLGLQLVAGAPAPQQLDGRTCGPTALTTARMLRDPALA
                     DWVRTGDKHGRAFPNGPSAALRLAAYHSLVHARTNALVGPGGRLQVPWPRALGTTPWG
                     VCRELESGAADPGARYRIVPVRRAGEARLAALVGHLAEHLRPGRPGVLFVGSATLPRH
                     VALLVAGPDGSVLVHDPSAGSVSELDVATLADPRTAVAGWTHPWFLIGPVG"
     gene            complement(5217..7844)
                     /gene="valS"
                     /locus_tag="PLK64_07015"
     CDS             complement(5217..7844)
                     /gene="valS"
                     /locus_tag="PLK64_07015"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPV79530.1"
                     /translation="MTSTPLRPTSIPDRPSVDGLEEKWGAVWREEGTYTFDRAKAMAG
                     PRSAVFSIDTPPPTASGSLHMGHVFSYTHTDCMARYKRMAGFEVFYPIGWDDNGLPTE
                     KRVQNYYGVRGDASLPYIPDFQPPHQGDVASIKAANQIAISRQNFIELCDVLTVLDEE
                     AFEALFRRMGFSLDWSISYRTIDDHSRATAQQAFLRNLERGEAYTSEAPGLWDVTFQT
                     AVAQAELEAKDYPGAYHRVAFHRPDGSPVYIETTRPELIPACVALIAHPDDERYQPLF
                     GTTVTSPLFGVEIPVRAHPAAEPDKGAGIAMCCTFGDLTDVIWWRELQLPNRSLITRS
                     GRLDAETPDWLSGTGAELYAAKIAGATAFGAREATVAALRESGDLDGEPTPTMRKANF
                     YERGEKPLEIVSSRQWYIRNGGRDAALNAALVERGREIAFHPGFMRSRYENWVQGLNG
                     DWLISRQRFFGVPIPVWYRLDDQGEPVYDTPLVPREDQLPLDPAASVPEGFTAEQRGI
                     PGGFVADPDVMDTWATSSLSPQIAAGWRGDPDLFATVFPMDLRPQAHDIIRTWLFATI
                     VRAHHEHGTVPWTNAAISGWILDPDRKKMSKSKGNVVTPEALVKEHSADAVRYWAASG
                     RLGTDAAFDAGQIKVGRRLAIKILNASKFALSFGTVEGDLGAAVTEPLDRSMLTALAD
                     VVRSATAGFEAWDYTRSLEITESFFWTFCDDYLELVKDRAYGGQGEAAAASARAALRI
                     ALDVLLRLLAPILPYVTEEVWSWWQEGSVHRQPWPTLADVVGVQAADGTSGDPEVLTA
                     VGAALAAVRKVKSEAKMGMRAEVASMTLAVPAEVGERVVLAEADLRAAGRITAMTYAA
                     SDAVEVRDAQLIPVEKPTA"
     gene            8032..8571
                     /locus_tag="PLK64_07020"
     CDS             8032..8571
                     /locus_tag="PLK64_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV79531.1"
                     /translation="MTTTVPAPALPGTREWRLGLPAWIHDARGAELLLRFVTPADAGA
                     IRGLHARCSEQALRWRYFGGGAQVEHLLTWIFDETRSEAVTAWSQGQLVAVGNLLLPD
                     ASGVGEVAFLVEDAWQGRGIGSTLVDVVTDRGRDHGAGLLHADVSLDNGRMRVLLARR
                     GWVGKVVGGDYCMDLVLSD"
     gene            complement(8541..9338)
                     /locus_tag="PLK64_07025"
     CDS             complement(8541..9338)
                     /locus_tag="PLK64_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017824727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPV79532.1"
                     /translation="MFRHAPAADLAGLVRHIWVPVWDIPAGEEWEQHVLNYPCCQVVV
                     GSDYARFYGVVRGLSRVTLAGRGWVVGVGLQPGAGWLVAGRPVSALTDTWVDLDEMGM
                     PWSALVPAVRSVMSPDPSDALAQRRAAGILEDAVRRHVVIDEAGLLVNAIVDAVESDQ
                     SLLRVDQLAARFALTERSLQRLTRRRLGLTPRWLVQRRRLHEAADRLRRGETDLAGLA
                     AYLGYADQAHLSREFKAVTGHTPATFRATATRADPAGDQSESTRSMQ"
     gene            9494..10039
                     /locus_tag="PLK64_07030"
     CDS             9494..10039
                     /locus_tag="PLK64_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HPV79533.1"
                     /translation="MTTTTVTPYRAALTPLSDIVATVTDWAAPSPCEGWSALDVLDHI
                     LTTQRALLTERGLTLPPPADLAADPVAAWAAHAATVAGLLDDPAIAGIAYDGHFGPTT
                     LGETVERFYVFDMLVHRWDLARAAGSETSFTDAELDRIDESAQSFGPVLYMEGICRPA
                     VPAASDADRQTRVLATLGRRA"
     gene            10095..>10285
                     /locus_tag="PLK64_07035"
     CDS             10095..>10285
                     /locus_tag="PLK64_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPV79534.1"
                     /translation="MDHVYFPVIGIAKTLFALQGLRFTVTGAQNVPKHGGAVLAINHT
                     GYLDFTYAGNAVLPRGRFV"
BASE COUNT         1561 a         3585 c         3568 g         1571 t
ORIGIN      
        1 ctcgatcctc gggctaccgg ccgcggggtg ggtcgtccat cggttcggcg ccgcgcgcac
       61 ggtccttgcc ggagtcatcg cctcggtcct cgcgctggcg acggtcggtg ccaccaccga
      121 gatcctgcac tcccggccgc tcaccatggc cgcgctcttc ttcgcgggcc tggggatcgg
      181 catctgggac atgtcgatga acctcgaagg cgccaccgtc gagcgcgcgg cgggacgggc
      241 gatcatgcct cgcttccacg ctgcgttcag cgccggcacg gtgttctcgg ccttggtcgg
      301 cagcggcctc accgctctgg gcgttggcgt gctgccgcac ctggcgggca cctcgctcgt
      361 cgttctcctc gccgcgctct gggcgacgcc gcggttcctc accacggggg cgggggcgca
      421 cggcataccg gatgccgctg actccaccgg cacggaaccg tcgacgtcgg ggctggggat
      481 gcgtgcggcc tggaccgagc gtcggaccct gctgattggg ctcgtcgtcc tcgtcgcggc
      541 gttcactgag ggcagcgcca acgactggct ggcaatcgcg ttcgttgacg gccaccacct
      601 tcccgcctgg gccggggtcc tcgcctttgc gacgttcctg gccagcatga ccgtcggccg
      661 gatcgccgga acgaagttgc tggaccgtta cggtcgagtg cgggtgctgc gcgcgacctt
      721 cctcaccgca gtcctcggct cggtcctcgt ggtcttcggc gggccctggc tggcgttcct
      781 cggtgcggcc ctgtggggtg tcggcgcgag cctcggcttc cccgtgggca tgtccgccgg
      841 agccgatgac ccagcgcgcg cagcggcccg ggtgagcgtc atcagcacca tcgggtatgg
      901 cgcgttcctc ggtgggccgc cgctgctggg cttcctcggc gaccacatcg gggtgcttcg
      961 ggccatgctc gtcgtcgggt tggccgtcct gctggccttt gccgccctgc cggccgtgcg
     1021 ggagccggct ccggaggaag acttcccgca aatctcaccg caggttgggt tccccgacgg
     1081 ctcctgatgc gtcacgatgg gagttccctc acgtgcggag gtggcccatg gacgcggaca
     1141 tcgcgagtta cctggacaag gtcaaacaac ggcgctcgga gctcgggcag tcgatggccg
     1201 aactgcagga ggtcatcgac gaacccgtcg gtgacgccga gcactggcgc gacgtggccc
     1261 gcgccgcagc agcggatgtg cgtcgcgacc tcgacgaaca catcgccgtg accgaagcga
     1321 acggcgggct ctacgactcg ctgcgcgcgg aggcggccag gctggccggg ccactgcgtc
     1381 acctgcaggg cgagcacccc gacctcgtcg ccctggcgga cgagttgatc gccgtcctgg
     1441 atcgacctgc tgccgccgac cgggccgacg cccccgagct ggtcaagaag ctcctgcgcc
     1501 ggctgtctca ccaccgacag cgaggcgccg acttggtgtt cgaggcctac caggtcgacg
     1561 tcggaggaac cggctgaccc gtgcccgtgt gcgctcaggg cgaacacgcg gcataccggg
     1621 attgtcggcg cgaccccgga cgttagggtc gggagagcgg gcaagccgtc ccgcaccgac
     1681 cgacgcatgg agtcacaggt gagccagagc aacgaggtcg tcatggccgg ggcgccgatg
     1741 cccggcagcc tcgacgatca catctacaac cggctgctca aggagcggat catcttcctg
     1801 ggctccgagg tccgggacga gaacgccaac gcgatctgcg cgcagatgct tcttcttgcg
     1861 gccgaagacc ccgagaagga catctggctc tacatcaact ccccgggtgg gtcggtgacc
     1921 gctggcatgg cgatcttcga caccatgcag tgggtgccca acgatgtggc caccgtcgcg
     1981 atggggatgg cggcctcgat gggccagttc ctcctctcgg ccggcacgcc gggcaagcgc
     2041 tacgccacgc cgcacgcccg agtgatgatg caccagccct ccggtggcat cggtggcacg
     2101 gcctcggaca tcaagatcca ggccgagcag atgctgcaca tcaagaagca gatggccgcg
     2161 ctcatcgccg agcacaccgg ccagaccctc gagcagatcg agcgtgactc cgaccgcgac
     2221 cgctggttca ccgcggagga ggccaaggcc tatggcttcg tcgaccacgt cgtcgagcgg
     2281 gcctcgagcg cggcacaccg cgccgacaac cccgtgaccg gcaactgacg cccgagacga
     2341 cggagagacc gaccatgacc atgaacgcag ggatggcccc gatggccccg atgggccgct
     2401 acatcctgcc ccagttcgag gagcgcacgt cctacgggat gaagcgcctg gacccctaca
     2461 cgaagatgtt cgaggaccgc atcatcttcc tgggtgtcca ggtcgacgac gcctcggccg
     2521 acgacatcat cgctcagctc atcgtgctgg agtctcagga ccccgaccgc gacatcctca
     2581 tgtacatcaa cagccccggt ggctcgttca cggcgatgac cgcgatctac gacaccatgc
     2641 agtacatccg gcccgacatc cagacgttcg tcgtcgggca ggccgcctcg gcggctgctg
     2701 tgctgctggg tgccggtgca ccgggcaagc gttacgccct gcccaacgcg cgcatcctca
     2761 tccaccagcc ggccctcgcc ggtggtgact acggccaggc ctccgacatc gagatccagg
     2821 ccaacgaggt gctgcggatg cgcacctggc tggaggagac gctggccctg cacaccggcc
     2881 gcaccgccga gcagacggcc cgcgacatcg agcgcgacaa gatcctcagt tcggccgagg
     2941 ccaaggagta cggcctcatc gacgaggttc tgctctcgcg caagggctcc gcacagaagt
     3001 gacctccgca ccggtatgcc gcccgctcac ccaggcgcgc ggcataccgg tgtcgtttgt
     3061 ccgccctcag cggacaatcg ctgacgccgg gtggatccgg gcgtgtaatg aagagcgcaa
     3121 ccggcgtggg gcaggtagcg tcggccttgg acctgaccgc attgggcggt cacgacagca
     3181 ggaggaccca gatggcgcgc gtgggtgacg gcagcgatct gctcaagtgc tccttctgcg
     3241 gcaagagcca gaagcaggtc aagaagctca tcgcgggccc tggcgtctac atctgcgacg
     3301 agtgcatcga tctgtgcaac gagatcatcg aggaggagct cgccgagacc tccgagctgg
     3361 gcctggacga gctgcccaag ccgcgcgaga tcttcgactt cctcggcacc tacgtcgtcg
     3421 gccaggacat cgccaagaag gccctcgcgg tcgcggtcta caaccactac aagcggatcc
     3481 aggcggggga ggggatgggc cgcaagggtg aggacgcggt cgacatcgcc aagtccaaca
     3541 tcctgctgat cggccccacc gggtgcggca agacctacct cgcccagacc ctggcccgca
     3601 tgctcaacgt gccgttcgcc atcgccgacg cgacggcgct gacagaggcc ggatatgtcg
     3661 gcgaggacgt cgagaacatc ctcctcaagc tcatccaggc cgccgactac gacgtcaaga
     3721 aggccgagac gggcatcatc tacatcgacg aggtcgacaa gatcgcccgc aagagcgaga
     3781 acccgtcgat cacccgcgac gtctccggtg agggcgtgca gcaggcgctc ctgaagatcc
     3841 tcgagggcac gaccgcctcg gtgccgccgc aaggtggccg caaacacccg caccaggagt
     3901 tcatccagat cgacacgacg aacgtcctgt tcatcgtcgg cggcgctttc gccgggctcg
     3961 agaagctcat cgaggcacgc tcgggcaaga agggcctggg cttcgggtcg cagttgcacc
     4021 tggccaagga agagggctgg tcctacgccg acgtcatgcc cgaagacctg atgaagttcg
     4081 ggctcatccc cgagttcatc ggccggcttc cggtcatcac gacggtgact ccgctggacc
     4141 gggcggccct cgtcgcgatc ctcagcgagc cgcgcaacgc gctcatcaag caataccagc
     4201 ggatgttcga gatcgacggc gtcgagctcg agttcacccc cgaggccgtt gaggcggtgg
     4261 ccgagcaggc cctgctgcgc ggcaccggcg cccgtgggct gcgggcgatc atggaggaag
     4321 tcctccagcc ggtgatgttc gacgtcccca gcgagaacga catcgcccgg gtcgtcatca
     4381 cccgcgaggt cgtgctcgac aacgtcaacc cgacgatcgt gcgccgcgag gccgctcgcg
     4441 tggtcaagca cgcgccgcgt cgccagtccg cctgagcatt cgcccgcccg cggtcgccgc
     4501 tcacagccct catgcccgcc ttcggctgac gtcgggtcgg tgtcccggtg gatcgtgact
     4561 cgcagcccct cgggctccaa ctcgtggccg gagcccccgc gccgcaacag ttggacggac
     4621 gcacctgcgg gccgacggcg ttgacgacgg cccggatgct gcgagatccc gccttggccg
     4681 attgggtccg caccggggac aagcacggtc gagcctttcc caacgggccg agcgcggcgt
     4741 tgcggctcgc ggcataccac tcgctcgtcc atgcccgcac gaacgcgctc gtcggccccg
     4801 gcgggaggct gcaggtcccg tggccgcgcg ctttgggcac cactccttgg ggggtatgcc
     4861 gtgagctcga gtcgggcgcg gccgacccgg gtgcgcgcta tcggatcgtg ccggtgcgcc
     4921 gggccgggga ggctcgcctg gccgcgttgg tggggcacct ggccgagcac ctgcgcccgg
     4981 gccgacccgg tgtgctcttc gtcgggagcg ccaccttgcc ccggcacgtc gcgttgctcg
     5041 tcgccggtcc cgacggctcg gtgctggtgc acgaccctag cgccgggtcc gtgagcgagc
     5101 tcgacgtcgc gacgctggcc gatccacgca cggccgtcgc cgggtggacc cacccgtggt
     5161 tcctcatcgg ccctgtcggg tgacgacagg gccgatgtgc cgaccggacc gacgggtcag
     5221 gcggtgggct tctccacggg gatgagctgg gcgtcgcgga cctcgacggc gtcggacgcg
     5281 gcatacgtca tcgcggtgat gcgtcccgcg gcgcgcaggt cggcctcggc gagcacgacc
     5341 cgctcgccca cctcggccgg cacggcgagg gtcatcgagg ccacctcggc gcgcatcccc
     5401 atcttggctt cggacttcac cttgcggacg gcagcgagcg cggctccgac ggcggtgagg
     5461 acctcggggt cacccgacgt cccgtccgcc gcctgcacac ccacgacatc ggcaagcgtc
     5521 ggccagggct gccggtgcac cgaaccctcc tgccaccagg accacacctc ttcggtgacg
     5581 tagggcagga tcggcgcgag gagccgcagg agcacgtcga gcgcgatccg cagcgcggcc
     5641 cgggccgacg cggccgccgc ctcgccctgc ccgccgtagg cccggtcctt gaccaactcg
     5701 aggtagtcgt cgcagaacgt ccagaagaac gactccgtga tctccagcga ccgggtgtag
     5761 tcccaggctt cgaatcccgc tgtcgcagaa cgcaccacgt cggccagcgc cgtaagcatc
     5821 gaccggtcga gcggctcggt caccgcggcg ccgaggtcac cctcgacggt cccgaacgac
     5881 aaggcgaact tgctggcatt gaggatcttg atggccagtc ggcggcccac cttgatctgc
     5941 ccggcgtcga acgccgcgtc tgtccccagc cgcccggagg ccgcccaata gcgcaccgcg
     6001 tcggccgagt gctccttgac cagagcctcg ggggtgacga cgttgccctt ggacttgctc
     6061 atcttcttgc ggtccgggtc gaggatccag cccgagatcg ccgcgttggt ccacggcact
     6121 gtgccgtgct cgtggtgggc gcgcacgatc gtcgcgaaca gccacgtgcg gatgatgtcg
     6181 tgcgcctgcg ggcgcaggtc catggggaag accgtcgcga acaagtcggg gtcgccacgc
     6241 cagccggccg cgatctgggg ggagagcgac gaggtcgccc acgtgtccat cacgtcgggg
     6301 tcagcgacga aaccccctgg tatgccgcgc tgctcggcgg tgaagccctc cggcacggat
     6361 gcggcggggt cgagcgggag ctggtcctct cggggtacga gaggggtgtc gtagaccggc
     6421 tcaccctgat cgtcgagtcg ataccagacg gggatcggca cgccgaagaa ccgctgccgc
     6481 gagatgagcc agtcaccgtt gaggccctgc acccagttct cgtagcggct gcgcatgaag
     6541 cccgggtgga acgcgatctc gcgcccccgc tctaccagcg cggcgttgag tgcggcgtcg
     6601 cgcccgccgt tgcggatgta ccactggcgc gaggacacga tctccagcgg cttctcgccc
     6661 cgctcgtaga agttggcctt gcgcatcgtc ggggtcggct caccgtcgag gtcgccgctc
     6721 tcccgcaggg ccgcgacggt ggcttcacgc gcgccgaacg ccgtcgcgcc ggcgatcttc
     6781 gcggcataca actccgcacc cgtccccgac aaccagtccg gcgtctcggc gtcgaggcgt
     6841 ccggaacggg tgatgaggga gcggttgggc agctgcagtt cccgccacca gatgacgtcg
     6901 gtgaggtcac cgaaggtgca gcacatcgcg atgccggcgc ccttgtcggg ctctgccgcc
     6961 gggtgggctc ggaccggaat ctcgacgccg aagagcggcg aggtgaccgt cgtgccgaac
     7021 aacggctggt aacgctcgtc gtcggggtgg gcgatgaggg ccacgcaggc ggggatgagc
     7081 tcgggccggg tcgtctcgat atacaccggc gacccgtcgg gccggtggaa ggcgacccgg
     7141 tgataggcgc cggggtagtc cttggcctcg agctcggcct gggccaccgc tgtctggaac
     7201 gtcacgtccc agagcccggg tgcctcagat gtgtaggcct cgccgcgctc caggttgcgc
     7261 aggaacgcct gctgggctgt cgcccgcgag tggtcgtcga tcgtgcggta ggagatggac
     7321 cagtcgagcg agaaccccat tcgccggaag agtgcctcga aggcctcctc gtcgaggacg
     7381 gtcaacacgt cgcacaactc gatgaagttc tgccggctga tggcgatctg gttggccgcc
     7441 ttgatcgagg cgacgtcgcc ctggtgcggc ggctggaagt cggggatgta gggcagcgac
     7501 gcgtcgccgc gcacgccgta gtagttctgc acccgcttct cggtcggcag gccgttgtcg
     7561 tcccacccga tcgggtagaa gacctcgaac ccggccatcc gcttgtaacg ggccatgcag
     7621 tcggtgtgcg tgtaggagaa cacgtgaccc atgtgcaggc tgcccgaggc cgtgggcggc
     7681 ggcgtgtcga tcgagaagac ggcgctgcgc ggcccggcca tggccttggc ccggtcgaac
     7741 gtgtaggtcc cctcctcgcg ccataccgcg ccccacttct cctcgaggcc gtcgaccgag
     7801 gggcggtcgg ggatcgaggt cgggcgaagc ggcgtgctgg tcatgccagg catcttctca
     7861 gaaccggcag gagcctccga accggtttcg gtgtggaccg tccggcgggt gtgatcgggc
     7921 ccaccagtgg cgagggtcgc aggtccctgg accgggccgt gagagcggtt acgttaccga
     7981 gtggtagcag gctgctcgcc tgtcgcccgc cgatcaacga ggaccagcgc catgacgacc
     8041 acagttccgg ccccggccct gccggggacc cgcgagtggc gccttggcct gccggcctgg
     8101 atccacgacg cgcggggcgc cgagttgttg ttgcgcttcg tcacgcccgc cgacgcgggg
     8161 gcgatccggg ggctgcacgc gcggtgttcg gagcaggccc tgcggtggcg ctacttcggg
     8221 ggcggtgctc aggtcgagca cctgctcacc tggatcttcg acgagacccg cagcgaggcc
     8281 gtcaccgctt ggagtcaagg gcagctcgtc gcggtgggca acctgttgtt gcccgacgcg
     8341 tcgggtgtcg gcgaggtggc cttcctcgtc gaggacgcct ggcagggccg ggggatcgga
     8401 tcgacgctgg tcgacgtcgt caccgaccgg ggccgggacc acggtgcagg cctgctgcat
     8461 gccgacgtgt cgctcgacaa cggccggatg cgggtgctgt tggcccggcg cggctgggtc
     8521 ggcaaggtcg ttggcggcga ctactgcatg gacctcgtgc tctccgactg atcgccagcc
     8581 ggatcggccc gggtggccgt cgcgcggaag gtcgcgggag tgtgcccggt cacggccttg
     8641 aactctcgac tcaggtgcgc ttgatcggca taccccaggt atgccgccag ccccgcgagg
     8701 tcggtctcgc cgcggcgcag gcggtcggcc gcctcgtgca ggcgtcggcg ctggaccaac
     8761 caccggggcg tcaaccccag gcgccgacgg gtcaaccgct ggagggagcg ctcggtgagg
     8821 gcgaagcggg cggcgagttg gtcgacccgc agcagcgact ggtcgctttc gaccgcgtcg
     8881 acgatggcgt tgaccagcag ccccgcctcg tcgatgacca cgtgccggcg aacggcgtct
     8941 tcgaggattc cggccgcccg ccgttgggcg agggcgtcgg acgggtcggg ggacatcacc
     9001 gaccggacgg cagggaccag ggccgaccac ggcataccca tctcgtcgag gtccacccac
     9061 gtgtcggtga gtgccgagac gggtcggcca gccacgagcc accccgcgcc cggttgcaga
     9121 ccgacgccca ccacccaccc gcgcccggcc agggtcaccc gggacagacc acgcaccacg
     9181 ccgtagaagc gggcgtagtc ggagcccacc accacctgac aacaggggta gttgagaacg
     9241 tgttgctccc attcctcgcc ggctgggatg tcccagacgg gcacccaaat gtggcggacc
     9301 agtccggcga ggtcggccgc cggagcatgg cggaacaccg ggggactcgg ccggccctgc
     9361 acgcgcaggt gcgccttggc taccgggtga tccggggact gctcggatgc ggacggctga
     9421 gggcggctgc cgatgtcggt ttgtcgggaa tcttcaagac cggtcagtgc cactcccttc
     9481 acgcttgggc gtcatgacga caaccaccgt gacaccgtac cgagccgccc tgacacctct
     9541 cagcgacatc gtcgccacag tcaccgactg ggctgcgccg tcgccgtgcg aggggtggag
     9601 cgccctggac gtcctggacc acatcctcac gactcagcgt gctctgctca ccgagcgcgg
     9661 cctgaccctg cccccgcctg ctgacctcgc ggccgacccc gttgcggcct gggccgccca
     9721 tgccgccacg gtggcggggc tgctggatga cccagccatc gccgggatcg cctacgacgg
     9781 acacttcggg ccgaccacgc tcggggagac ggtggaacgc ttctacgtgt tcgacatgct
     9841 cgtccaccgt tgggacctgg ctcgggcggc cggcagcgaa acgtccttca ccgacgctga
     9901 actcgaccgg atcgacgagt cggcgcagtc gttcgggccg gtgctctaca tggagggcat
     9961 ctgccgaccg gccgtgccgg ccgcgtcgga cgccgaccgg cagacccgag tgctcgccac
    10021 gttgggccgc cgcgcgtgag gtgagcgggc ggcataccag cgggtatgcc gcccacccac
    10081 ctacagtgtg gggcgtggac cacgtctact tccccgtcat cgggatcgcc aagaccctct
    10141 tcgccctcca ggggctgcgt ttcaccgtga ccggtgcgca gaacgtgccc aaacacgggg
    10201 gagcggtcct ggcgatcaac cacaccggct acctcgactt cacctatgcc ggcaacgccg
    10261 tgctgcctcg cgggcggttc gtcag
//
LOCUS       DAOMZY010000095        10226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_6178_length_10226_cov_6.120440, whole genome shotgun sequence.
ACCESSION   DAOMZY010000095 DAOMZY010000000
VERSION     DAOMZY010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 10226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10226
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6178_length_10226_cov_6.120440"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..3954)
                     /locus_tag="PLK64_07040"
     CDS             complement(4..3954)
                     /locus_tag="PLK64_07040"
                     /inference="COORDINATES: protein motif:HMM:NF009589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HPV79535.1"
                     /translation="MSSTLLPPLSLEDKFTVDEGIIHLTGVQALVRLPMVQRRLDLAA
                     GLNTATFISGYPGSPLGGFDLELQRRRGLMDAHHVVHQMGVNEELGATAVMGSQLAMQ
                     LPGPRFDGVLGLWYGKANGFDRALDALRQANLAGTVRTGGALALVGDDPTAKSSPTPG
                     ASEVAAAAIMMPMLYPGDVQEVLDLGQHAVALSRSCGLWVAVKMSTTVADGSGSAVVA
                     PGRVQPIMVDIEENGRPYLHRPSTHMYGARVMEMEHSMVYGRMRAALAYARANDVNRI
                     TLRTDADRVGIISSGKTYFEMRQTLRELGLDDRELQRLGVRLLQLRMPYPLEGGIIRE
                     FAAGLEEILVLEDKRPFVELFVKDELYGLADRPLVLGKLDEDGKWLVPIHAELDTVSI
                     ARLVAERLLKRAAPGELAVLEERLERITRPRSLAPLAMSRTPYFCSGCPHNSSVAGVP
                     AGTTVGAGTGCHVLAVFMRPDEVGDILGITAMGNEGAQWIGASPFCSLPHFIQNLGDG
                     TYAHSGSLAIRASVAAGVNITYKLLYNDHVAMTGAQPAIGISGVPDLVTTLLAEGVRQ
                     VIITTEDLRRYAGVRLPDGAAVWDRSRLGEAVSVLSAVEGVTVLVHDQECATEKRRRR
                     KRGTLEEPKTRILINEAVCEGCGDCGIKSNCLSVHPVQTELGRKTQIHQPSCNLDYSC
                     VQGNCPSFVKVVPGTAVRPRRGASATLGADDLPEPTLRVPADEFGMRIAGIGGTGVVT
                     VAQTLATAALLDGRFVRGLDQTGLAQKGGPVISDLRITTSPMDQANKLTVADCDLFLA
                     CDVLVGAQQQLLTVTDSERTVAVTSTAQVPTGQMVVDTQLAFPDVDGLVARIDASTRS
                     DLNVALDAPGIAERLFGTDQVANILLVGAAYQIGALPLRAESIEAALTLNGVAVETNL
                     QAFRRGRQAVADRAALDELVGASRVPAGAADLSLSATPTSGSSGSRPGIVLPRELRAI
                     TDLVGAEPGSELARMVAVRVPMLVDYQDAAYARRYAVRLEAVRAAEAQRTPGRSALAE
                     AAAFSLFKLMAYKDEYEVARLHRDPAFQADLAERFGGDAAYSVMLLPPVLKTIGLKRK
                     VGLSRPVAKVAFGALAPMKRLRNTRFDPFGRDHVRVVERALIAEYDALLDEIIQRLSV
                     ENHAVAVELAALPDLVRGYDEVKMRNVDRYREQVTRLRVQLTDGRLIVGRLDVDGATE
                     AHREAESQAGGAAPRPAAHEHAGSSTAWAGADSERGASASDPSPRPKRRRRSMHDNLE
                     HDAASEGRIEPSTAALMEGAPPGGTSGRVQPPRRTRTGVHDEGTNEGDTGNLPG"
     gene            complement(4008..4520)
                     /locus_tag="PLK64_07045"
     CDS             complement(4008..4520)
                     /locus_tag="PLK64_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79536.1"
                     /translation="MDLGNLLGGIDTDAIADVVKVVVDNRAMIEQLGRLPELFGQFAD
                     NLQGAGQEAGSAALALVGADGTSGARGALASAATALAGIVTTLDSGIARIAETAEGAA
                     KVPLMGGPAGHLAEAAKDMGESTAQLGTLAASMGSIAEVLDEVASALRNVGVYLTDSG
                     TQARGFVGTS"
     gene            complement(4587..6068)
                     /locus_tag="PLK64_07050"
     CDS             complement(4587..6068)
                     /locus_tag="PLK64_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPV79537.1"
                     /translation="MGDARPSDRLGLVVVHDADAAPDADERWTFGELDEAVRRIAAGL
                     LGVGLVPGDRVLLRMGNTSDAALAFFGAVAAGLVAVPTSAMLTGDEVAAVVADARPSL
                     VIAAPEHAATVPHGIPLVGPEHLAKWRADLAPAPWRDSRADDPAFLVYTSGTSGEPKG
                     VLHAHRSAWGRRPMYAGWEGLRPGDVMLHAGAFNWTYTLGVGLTDPWANGGTAVVYNG
                     PRDPAVWGRLVKRCGATIFAAVPGVYRQLLASGALAPADVATLRHGLTAGEALSREMY
                     AAWQEATGLPLYEALGMSEISTYVSSGPTVPTRPGSPGKPQEGRCIAILPVEPTDPAE
                     GDAPLPTGHTGLLAIHRTDPGLMLGYWERPDEDAATRRGEWFVGGDLAHLDDDGYLIH
                     HGRADDVMNPGGFRVSPQEVEHVLAAAPGVADVGVTEVEIRAGVRVVGAFVVARPQGC
                     DPEAVRAYAAEHLAAYKVPRVVRLVSTLPRTANGKLVRRELSS"
     gene            complement(6189..7400)
                     /locus_tag="PLK64_07055"
     CDS             complement(6189..7400)
                     /locus_tag="PLK64_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPV79538.1"
                     /translation="MSNESGSEGTPAPRGHARVPGDSDRRPTWVALAQSKRVLWVLVA
                     AGLALLIGVSVFQALGSFLFLLLLAWLLSIAMEPMVGWLIRRGMSRAMASAAVLFGLM
                     AVTVGIAAMFGQVFISQAQELKDAFPDAVRQAVAWVNSTFNTSFNTDQIYTALQLSPE
                     RISELAQRFGGGLLGVFGAVVTFIFNLVTILVFAYYLSADSIKLRRTIGSYLPPRYQP
                     MLMTIWTIAIDKTGGYVVSKVILSTFSSVAYALFFLILDVPFWLPLGVLMGISGQFIP
                     IIGTYIGVVVPALFSLLRDPLDAVWIVVFATIYQQFESYWLTPKISNRTMDVHPAVAL
                     GSVFVGVALMGPIGAVIGIPVAAAVITIADTFRRRHELLPELETLQDDDGGSDGDSAD
                     AGPPTGAAETA"
     gene            complement(7611..7796)
                     /locus_tag="PLK64_07060"
     CDS             complement(7611..7796)
                     /locus_tag="PLK64_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BldC family transcriptional regulator"
                     /protein_id="HPV79539.1"
                     /translation="MLGAGEERDPLLTPGEVAGLFRVDPKTVTRWAKAGKLTAIRTLG
                     GHRRYRSSEVHRLLGGQ"
     gene            8066..8305
                     /locus_tag="PLK64_07065"
     CDS             8066..8305
                     /locus_tag="PLK64_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009213040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3073 domain-containing protein"
                     /protein_id="HPV79540.1"
                     /translation="MGRGRAKAKQTKVARELKYFSPDTDLSALERELHSATHRGGQSF
                     NQTDDVDDDEDDEEEDDDADDGEVDDDYASWAAGR"
     gene            complement(8419..8958)
                     /locus_tag="PLK64_07070"
     CDS             complement(8419..8958)
                     /locus_tag="PLK64_07070"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPV79541.1"
                     /translation="MSDATPSAGGMDPALDPPAVATVGTERELLQAWLDFHRQTLRWK
                     CSGLTPEQLCEASAPPSRMSLAGLMRHMTDVEVWWFGRVAVGDESATDRYPGQDEDLC
                     EATAPTVAADWEAFEAAIRRSDEVFATLNLDATAAWMHPRRGPVSNRWILIHMVEEWA
                     RHNGHADLIRERIDGAAGD"
     gene            complement(8994..10097)
                     /gene="purM"
                     /locus_tag="PLK64_07075"
     CDS             complement(8994..10097)
                     /gene="purM"
                     /locus_tag="PLK64_07075"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPV79542.1"
                     /translation="MTYAAAGVDVEAGDKAVELMKESIRRATRPEVLGGLGGFAGLFD
                     ASALARMTRPILATSTDGVGTKVAIAQALDRHDTIGFDLVGMVVDDIVVCGAEPLFMT
                     DYIATGKVVPERIAAIVGGIARACEQAGVALVGGETAEHPGLLDPDEYDVAGAATGVV
                     EYAGILGPQRVEAGDVVLALASSGLHSNGYSLVRRVIAAAGWSLDRHVEEFGHTLGEE
                     LLTPTRVYAADLLSLIRTPGIDIHALSHVTGGGLAANLARVLPRGSYARVDRSTWTPP
                     AVFEVIGALGRVPRSDLERTLNLGIGFVAVLPAAHAAAAEQVCRARGIPAWVLGEVGE
                     EATWTPQPDVEVARGAKGVDGGAVQVVGEHPKP"
BASE COUNT         1616 a         3586 c         3554 g         1470 t
ORIGIN      
        1 cggtcagccg gggaggttgc cggtgtctcc ctcgttggtg ccctcgtcgt gaacgccggt
       61 ccgcgtccgc cgcggcggct ggacccgtcc gctcgtcccg ccgggagggg caccttccat
      121 gagggcggcc gtgctgggct cgatccggcc ttcgctggct gcgtcgtgtt cgaggttgtc
      181 gtgcatcgag cgccgccgcc gcttgggccg gggcgagggg tccgacgcac tggcgccgcg
      241 ctcggaatcg gcaccggccc acgccgtgct cgaccctgcg tgctcatgcg ccgcaggccg
      301 aggcgcggcc ccgcccgcct ggctctcggc ctcgcggtgc gcctcggttg cgccgtcgac
      361 gtcgagccgc ccgacgatca aacggccgtc ggtgagctgg acccgcaacc gggtcacctg
      421 ctcgcgatag cggtcgacgt tgcgcatctt gacctcgtcg taccctcgca ccaggtccgg
      481 cagcgccgcc aattcgactg ccacagcatg attttcgaca ctcagccgct gaatgatctc
      541 gtccagcagc gcgtcatact cggcgatgag ggcgcgctct accacccgta catggtcacg
      601 cccgaacggg tcgaaccggg tgttccgcaa ccgcttcatc ggcgccagcg ccccgaaggc
      661 aaccttcgcc accggccgcg acaaccccac cttgcgcttg aggccgatcg tcttgagcac
      721 gggcggcagg agcatgacgg agtatgccgc gtcgcccccg aaccgctcgg ccagatcggc
      781 ctggaaggcc ggatcccgat gcagccgggc aacttcgtat tcgtccttgt aggccatgag
      841 tttgaacagc gagaacgctg cggcctcggc cagcgcgctt cggcccggcg tgcgctgggc
      901 ctcggccgcg cggacggcct ctaggcgcac ggcataccgg cgggcgtatg ccgcgtcctg
      961 gtagtccacg agcatgggaa cccgcaccgc gaccatccgg gcgagctccg aacccggctc
     1021 tgcgccaacg agatcggtga ttgcacgtag ctcgcgcggc aggacgatcc ccggccgtga
     1081 ccccgacgac cctgaggttg gcgtcgccga cagcgacagg tcggcagcgc ccgcaggcac
     1141 gcgcgaggcg ccgacaagct catcgagcgc agcccgatcg gcaacggcct ggcgcccgcg
     1201 ccggaaggct tgcaggttgg tctcgacggc cacgccgttg agggtgaggg ccgcctcaat
     1261 cgactcggcc cgcaagggca gcgccccgat ctgatatgcc gcccccacca gcaggatgtt
     1321 ggcgacctgg tcggtgccga agagccgctc ggcgatgccc ggggcgtcga gggccacgtt
     1381 gaggtccgac cgggtggagg cgtcgatccg ggccacgagc ccgtcgacgt cggggaaggc
     1441 gagttgggtg tcgacgacca tctgcccggt cggcacctga gccgtcgagg tcaccgccac
     1501 cgtgcgctcg gagtcggtca ccgtcagcaa ctgctgctga gccccgacca ggacgtcaca
     1561 ggcgaggaag agatcgcagt cggcgacggt gagcttgtta gcctgatcca tcgggctggt
     1621 cgtgatccgc aggtcggaga tgaccggccc gcccttctgg gccaacccgg tctggtccag
     1681 accgcgcacg aagcgcccgt cgagcagggc cgcagtggcc aacgtctggg ccacggtgac
     1741 gacacccgtg ccgccgatgc cggcgatccg catcccgaac tcgtcggccg gcacccgcag
     1801 ggtcggctcc ggcaggtcgt cggcgccgag ggttgctgag gcgccacggc ggggccgcac
     1861 cgcggtgccc ggaacaacct tgacgaacga cggacagttg ccctggacac acgagtagtc
     1921 gaggttgcac gacggttggt ggatctgggt cttgcggccc aactcggtct ggaccgggtg
     1981 caccgacaga cagttcgact tgatgccaca gtcgccgcag ccttcacaga ccgcctcgtt
     2041 gatgaggatc cgggtcttgg gctcctcgag tgtcccgcgc ttgcgccgac gacgcttctc
     2101 ggttgcgcat tcctggtcgt gcaccaggac ggtgactcct tcgaccgcgc tgagaacgct
     2161 cacggcctcc cccaagcggg agcggtccca caccgcagcg ccgtcgggga gtctcacacc
     2221 ggcataccgg cgcaggtcct cggtcgtgat gatgacctgg cggaccccct cggccagcag
     2281 cgtcgtcacc aggtcgggga cgcccgagat gccgatggcc ggctgcgcgc cggtcatcgc
     2341 cacgtggtcg ttgtagagca gtttgtaggt gatgttgacc ccggcggcga ccgaggcacg
     2401 gatggctaaa gaccccgagt gggcgtaggt gccgtcgccg aggttctgga tgaagtgcgg
     2461 caggctgcag aagggcgacg cgccgatcca ctgggcaccc tcgttgccca tcgcggtgat
     2521 accgaggatg tcgccgacct cgtcggggcg catgaagacg gcgagcacgt ggcagcccgt
     2581 cccggcaccg accgtcgtcc ccgcggggac tcccgccacg gaggagttgt gggggcaccc
     2641 ggagcagaag tagggggtgc gcgacatggc cagcggcgcc agggagcgcg ggcgggtgat
     2701 ccgctccagc cgctcctcca gcaccgcgag ctcaccggga gcagctcgct tgagcagccg
     2761 ttcggcgacc aaccgggcga tcgacaccgt gtctagctcg gcgtggatcg ggacgagcca
     2821 cttgccgtcc tcgtcgagtt tgcccagcac gagcggccgg tcggccagcc catagagctc
     2881 gtccttgacg aacaactcga cgaaggggcg cttgtcctcc aggacgagga tctcctccag
     2941 cccggcggcg aactcccgga tgatcccgcc ctcgagcggg tagggcatcc gcaactgcaa
     3001 caggcgcaca ccgaggcgct gcaactcccg gtcgtccagc cccaactccc gcaaggtctg
     3061 gcgcatctcg aaataggtct tgccggacga aatgatcccc acccggtcgg cgtcggtgcg
     3121 cagggtgatc cggttgacgt cgttggcccg ggcataggcc agggctgccc gcatccggcc
     3181 atagaccatg gagtgctcca tctccatgac ccgggcgccg tacatgtggg tcgagggtcg
     3241 gtgcagatag ggccgcccgt tctcctcgat gtccaccatg atcggctgga cgcgccccgg
     3301 agccaccacc gcagacccgg acccgtcggc gaccgtggtc gacatcttca ccgccaccca
     3361 cagaccacac gaccgcgaga gggccacggc gtgctgcccc aggtcgagga cctcttggac
     3421 atcgcccgga tagagcatcg gcatcatgat cgctgccgcc gcgacctcac tggcccccgg
     3481 cgtcggcgac gacttggcgg tcgggtcgtc tccgaccagg gccaacgcgc cgcccgtacg
     3541 caccgtgccg gcgaggttgg cctggcgcag cgcatccagg gcccggtcga acccgttggc
     3601 cttgccgtac cagaggccca acaccccgtc gaaacgcggg cctggcaact gcatcgccag
     3661 ttggctgccc atgacggcgg tcgccccgag ctcctcgttg actcccatct gatggacgac
     3721 gtgatgggcg tccatcagcc cccggcgccg ctgcagttcc aggtcaaaac cgcccaacgg
     3781 cgagcctgga tagcccgaga tgaaggtggc ggtgttgaga ccggccgcga ggtccagccg
     3841 tcgctgcacc atgggcaacc ggacgagggc ctgcaccccc gtgaggtgga tgatcccctc
     3901 gtcgacggtg aacttgtcct ccagcgacag cggtggaagc agggtgctgc tcatctcggc
     3961 atcctatgtc cgcggcatac cggcaaccgg tgctgcgaac gcgcggatca ggacgttccg
     4021 acgaagcccc tggcctgagt gccgctgtcc gtgaggtaga caccgacgtt ccgcaaggca
     4081 gaagcgacct cgtcgagcac ctcggcgatc gaccccatcg aggccgccag cgtgcccaac
     4141 tgcgccgtcg actcgcccat gtccttggcc gcctccgcga ggtgcccggc cgggccaccc
     4201 atgagcggca ctttggcggc cccctcggcc gtttcggcga tccgcgcaat gccgctgtca
     4261 agcgtggtga cgatgccggc gagcgccgtc gcggccgacg ccagcgcgcc gcgggcgccg
     4321 gacgtgccgt cggcgccgac cagcgcgagc gcagcggatc cggcctcctg gccggccccc
     4381 tggaggttgt cggcgaattg cccgaacaac tcgggcaggc ggcccagttg ctcaatcatc
     4441 gcgcggttgt cgacgaccac cttgacgacg tcggcgatgg cgtcggtgtc gatcccgccc
     4501 agcaggtttc ccaggtccat gcgcgcatcc tagggccgcc ggcgcggccg gttctcggcc
     4561 cagaactgcc cgggacccag cggaagtcac gagctcaatt ctcggcgcac cagtttgccg
     4621 ttggcggtcc ggggtagcgt cgagaccagt cggaccaccc gcgggacctt gtatgccgcg
     4681 aggtgctccg cggcatacgc ccggaccgcc tcggggtcgc acccctgtgg gcgcgcgacg
     4741 acgaacgcgc cgacgacccg gaccccggcc cggatctcga cctcggtgac cccgacgtcg
     4801 gccacgccgg gtgcggcggc gaggacgtgc tcgacctcct ggggcgagac ccggaatccg
     4861 ccggggttca tgacatcgtc ggcccgcccg tggtggatca ggtagccgtc gtcgtccagg
     4921 tgggccaggt cgccgccgac gaaccactca ccgcggcggg tggcggcatc ctcgtcgggc
     4981 cgctcccaat agccgagcat gaggccgggg tcggtgcgat ggatcgccag caacccggtg
     5041 tggcccgtcg gcagcggcgc gtcgccttca gccggatccg tcggctcgac cggcaggatg
     5101 gcgatgcagc ggccttcctg cggcttgccc ggcgagccgg gccgggtcgg cacggtcggc
     5161 cccgacgaga cgtaagtgga gatctcgctc atccccagcg cctcgtagag cggcagcccg
     5221 gtggcctcct gccacgcggc atacatctcg cgcgagagcg cctcgccggc ggtgaggccg
     5281 tgtcgcaggg tggccacatc ggccggtgcg agcgccccgc tcgctagcag ttgccgatag
     5341 acgcccggaa ctgccgcgaa gatcgttgcg ccacaacgct tcacgagcct cccccagacg
     5401 gccgggtcgc gggggccgtt gtacacgacc gccgtcccgc cgttggccca cgggtcggtc
     5461 agcccgacgc cgagcgtgta ggtccagttg aacgcacccg cgtggagcat gacgtcaccc
     5521 ggtcgcagac cctcccaccc ggcatacatg ggccgccgtc cccacgcgga gcggtgggcg
     5581 tgcaggacgc ccttcggctc gcccgaggtc cccgaggtgt agacgaggaa ggccgggtcg
     5641 tcggctcggc tgtcgcgcca cggagcgggg gcgaggtcgg cgcgccactt cgcgaggtgt
     5701 tccggaccga caaggggtat gccgtgcggc acggtcgctg cgtgctccgg cgccgcgatc
     5761 accagggaag ggcgggcgtc ggcgaccacc gcggccacct cgtcgccggt gagcatcgcc
     5821 gaggtcggca cggcaaccag accggcggcg acggctccga agaaggccag ggccgcgtca
     5881 ctcgtgttgc ccatccgcag caggacccga tccccgggga cgagcccgac gcccagcagc
     5941 ccggcggcga tccggcgcac cgcctcgtcc aactcgccga aggtccagcg ttcgtcggcg
     6001 tccggcgcgg cgtcggcatc gtggacgacg acaagaccga gccggtcaga cgggcgggcg
     6061 tcacccacgg ccaagcagta gcgagccatg tcgaaccgct cgggaggccg gggacctcga
     6121 aatcccagcg agaccatctt tgggtccgcc cccatggtcg ccggccggct catcgctgca
     6181 ggcgcgagtc aggcagtctc ggccgcgccc gttggtggcc cggcgtcagc agagtcaccg
     6241 tcggagcctc cgtcgtcgtc ctgcagggtc tccaactcgg ggagcaactc gtggcgccgt
     6301 cggaacgtat cggcaatggt gatcaccgcc gcggcgacgg gaatgccgat gacggccccg
     6361 atcggcccca tgagggccac gccgacgaac accgacccga gggccaccgc tgggtggaca
     6421 tccatcgtcc ggttgctgat cttgggggtc agccagtagc tctcgaactg ctggtagatc
     6481 gtggcgaaca cgacgatcca cacggcgtcg agcgggtcgc gaaggagcga gaacaaggcg
     6541 gggacgacga caccgatgta ggtgccaatg atcgggatga actggccgga gatccccatg
     6601 agcaccccaa ggggcagcca gaagggcacg tcaaggatga ggaagaacag cgcgtacgcg
     6661 actgaggaga aggtcgacag gatgaccttc gagacgacgt agccgccggt cttgtcgatc
     6721 gcgatggtcc agatggtcat gagcatcggc tggtaccggg gcggcaggta ggagccgatc
     6781 gtgcggcgca gtttgatgct gtcggccgac aggtagtaag cgaagacgag gatcgtcacg
     6841 aggttgaaga tgaacgtgac gaccgcaccg aagacaccga gcaggccgcc gccgaagcgc
     6901 tgggccaact cggagatccg ctcgggcgag agttggaggg cggtgtagat ctggtcggtg
     6961 ttgaacgacg tgttgaacgt gctgttgacc cacgcgacgg cttggcggac ggcgtcaggg
     7021 aaggcgtcct tgagctcctg cgcctgtgag atgaagacct gaccgaacat ggccgcgatg
     7081 ccgacggtga cggccatcag cccgaacagg acggcggctg acgccatggc ccgactcatg
     7141 ccgcggcgga tgagccaccc gaccatcggc tccatggcga tcgacagcag ccaggccagc
     7201 agcaggagga acaggaagct gcccagcgcc tggaagaccg acacgccgat gagcagggcg
     7261 agtcccgcgg ccacgaggac ccagaggact cgcttggact gcgcgagggc gacccaggtc
     7321 ggccgacgat cgctgtctcc cggcaccctc gcgtgccccc ggggggccgg ggtgccctca
     7381 gaaccgctct cattgctcac agaaaccact gtagttgggc cgcggcgcag ggggcagaga
     7441 tggcgcccgg tatgccgcga ggctgttcag ggcagcacga atcgcagaac gtcgttaacc
     7501 cgcattatgc ggcgaacgtt ctgtttgctc tagccgtgcg gcgccttccc ccgaaggcac
     7561 tccgatcgct ccgcaccccc acgatgcgtc acgaccgcgt tccgcttggc ctactgcccg
     7621 ccgagcagcc tgtgcacttc cgagctgcgg tagcgccggt gtccgcccaa tgtgcggatc
     7681 gccgtcaact tgcccgcctt agcccatcgg gtgaccgtct tcgggtccac tcggaagagc
     7741 cctgccactt caccaggcgt gagcaagggg tctcgctcct cgcccgctcc caacatcatt
     7801 ctttggtact ccattcggat gaccccgcgt caatcccctg cgcgggacac tctcgcacga
     7861 ccgaggcacc tctggtggga aacgctccca actgatcacc aggaaggacc cgtcagacag
     7921 aaccgccaac gtcgggtagc gttgtcccca cggagacaca catttcccgg ggccgctccg
     7981 cggcgacccg ccgtgcactg ccgatcggca cgtgaccggc gggcctggcg atgccgcccc
     8041 ggcagacgca tgagggggtc acgccatggg gcgcggccgg gccaaggcca agcaaactaa
     8101 ggtcgcccgg gagttgaagt atttctctcc cgacaccgat ctcagcgcgc ttgagcgcga
     8161 actacacagc gccacccacc gtggcggcca gtcgttcaac cagaccgacg acgtcgatga
     8221 tgacgaagac gacgaggaag aggacgacga cgctgatgac ggtgaggtgg acgacgacta
     8281 cgcgtcgtgg gcggctggtc gctgaagcga ccggccctca cccgtagcgg cacctcatgc
     8341 ggtcctcgac cgtgagttgc tgacacccga ccgccggtcg ggcccaaggc atgccctcaa
     8401 gcgccgggga ctggcgactc agtcgccggc cgcgccatcg atccgttccc ggatgaggtc
     8461 ggcatgaccg ttgtgccgcg cccactcctc gaccatgtgg atgaggatcc accggttgct
     8521 caccggcccg cgcctggggt gcatccacgc ggctgtcgcg tccaggttga gcgtggcgaa
     8581 cacctcatcc gaccgacgga tggctgcctc gaacgcctcc cagtcggctg ccacggtcgg
     8641 tgccgtcgcc tcgcagagat cttcgtcctg gccgggatag cgatcggttg ccgactcgtc
     8701 ccccaccgcg acccgtccga accaccagac ctcgacgtcg gtcatgtgcc gcatcagccc
     8761 ggccagactc atccgcgagg gcggcgcgct ggcctcgcac agctgctccg gcgtcaaccc
     8821 tgagcacttc cagcgcagcg tctgacgatg gaagtcgagc caggcctgca gcaactcgcg
     8881 ctcggtgccg acggtcgcca ctgcgggcgg gtcgagggcg gggtccatgc cgcctgcgga
     8941 cggggtcgcg tcgctcatcg ccccaccgta ggccagtgcc gggcgcccgc gcctcagggt
     9001 ttcgggtgct ccccgacgac ctgcaccgct cccccgtcga cacccttcgc cccacgggcc
     9061 acctcgacgt ccggctgcgg cgtccacgtc gcctcctcgc cgacctcccc caggacccac
     9121 gccggtatgc cgcgcgcgcg gcatacctgc tcggcggcgg ctgcgtgcgc ggcggggagg
     9181 acggccacga agccgatgcc gaggttgagg gtgcgctcaa ggtcgctgcg gggcacccgg
     9241 ccgagtgcgc cgatgacctc gaagaccgcc ggcggggtcc aggtcgaccg gtcgacccgg
     9301 gcgtagctgc cgcggggcag gacgcgggcg aggttggcgg ccaggccacc gccggtgacg
     9361 tgcgagagcg cgtggatgtc gatgccgggg gtgcggatga gcgagaggag gtcggcggca
     9421 tacacgcgcg tgggagtgag aagctcctca cccagcgtgt gcccgaactc ctcgacgtgc
     9481 cggtccagcg accagccggc cgccgcaatg acccggcgga ccagggagta gccgttggag
     9541 tgcaggcccg aggacgcgag cgcgaggacg acatcgccgg cctcgacgcg ctgtgggccg
     9601 agaataccgg cgtattcgac aaccccggtc gcggcccccg cgacgtcgta ctcgtcgggg
     9661 tccagaagcc ccgggtgctc ggccgtctcg ccgccgacga gggcgacccc ggcctgctcg
     9721 caggcgcggg cgatgccgcc gacgatggcc gcgatccgtt cggggacgac cttgccggtg
     9781 gcgatgtagt cggtcatgaa gaggggctcg gcgccacaga cgacgatgtc gtcgacgacc
     9841 attccgacca ggtcgaagcc gatggtgtcg tgccgatcga gggcctgggc gatggcgacc
     9901 ttggtgccga ccccgtcggt cgacgtggcg aggatcgggc gggtcatccg ggcgagggcg
     9961 ctggcgtcga agagcccagc gaaaccgccg aggccgccga gcacctcggg tcgggtggcc
    10021 cggcggatcg actccttcat caactcgacg gccttgtcgc ccgcttcgac gtcgactcct
    10081 gccgcggcat acgtgatggg gcccggctcc gaggtcgagg tcgaggtcga ggtcgaggag
    10141 gctggcggcg gggtcgacgg agcagtcacg tgcttagcct agggtccccg ccagagtcag
    10201 gggtgcagca gggcgttctc ggcgcc
//
LOCUS       DAOMZY010000096        10187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_6213_length_10187_cov_5.076392, whole genome shotgun sequence.
ACCESSION   DAOMZY010000096 DAOMZY010000000
VERSION     DAOMZY010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 10187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10187
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6213_length_10187_cov_5.076392"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1615)
                     /locus_tag="PLK64_07080"
     CDS             complement(<1..1615)
                     /locus_tag="PLK64_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPV79543.1"
                     /translation="MWVRFVLGLLMTVVCLGIAGRRAYFLYRVGKTFQPLEAERRVDP
                     GSAVRAEVAEVAFQRKLLRWTVPGAAHFLVFWGFMVLLFTIVEAFIGLVKPDFQIPIV
                     GNFAWFGFFEDLFALLCVAALAVFTVLRIQQAPKNLGRRSRFFGSHIGPAWFVLFMIF
                     NVVWTLLAYRGASINAQELHEPANADKMGFLHGAFASQWFASILPKTNVEFWETFFLL
                     LSLAVLLGFTVFVTYSKHMHIFASIPNVAFARRPRALGALEPVYWQGTKVDFEDPGDD
                     DVMGVGKIEDFTWKGLLDFATCTECGRCQSQCPAWNTDKPLSPKVLIMGLRDHSLAKA
                     PYLLAAKNADGEVPEGGIPEAAAAEAARPLIGPEGDETAHVGEHGITGEGVIPFDMLW
                     SCTTCGACVEQCPVDIEHVDHIVDMRRYQVLMESAFPEEAGIMLRNLEHAGDPWGLGA
                     SARLEWAETLDFELRVYEGDKIPDDVEYLYWVGCAGALDDTAKRTARAVATLLHEAGV
                     EFMVLGEAESCTGDPARRMGHELHLQMLAM"
     gene            complement(1667..2629)
                     /locus_tag="PLK64_07085"
     CDS             complement(1667..2629)
                     /locus_tag="PLK64_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPV79544.1"
                     /translation="MSEVLVIAETTAAGVRKPTLELLTAARALGEPAAVVFGEASDAV
                     VAQLGEYGATKVYAVNAAEVGDYLSLPKAEAVVQIAGTANPVAILVTSGPEGKDVAAR
                     VAVRLNSGIVPDATVVALDGGNVVATQTVFAGRWQARSQIVRGPAVITMRPNATSAEA
                     AAASPSVETVDVAVSEAAKGAKITSREPKVSTGRPELTDAAVVVAGGRGVGSEEGFAV
                     IGQTADALGAAVGASRTVTDQHWAPHELQVGQTGKTVAPSLYLAAGISGAIQHRAGMQ
                     SSKTIVVVNKDPKAPLFQLADFGVVGDLHKVLPALIDEVGKRKG"
     gene            complement(2707..3486)
                     /locus_tag="PLK64_07090"
     CDS             complement(2707..3486)
                     /locus_tag="PLK64_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPV79545.1"
                     /translation="MKIVVLVKYVPEPTATWKFAPDLTVDRAGVEGRLSELDEYAVEQ
                     AAQLAEKGVASEVVFLTVGPAKASEGLRKALAIGGDKAVHVVDDAIHGSCALSTSLVL
                     AKALEKEGFDLVLTGMASTDAELSVVPSMVADRLGINQATFAGELSVEGGAVTIRRDS
                     DVASERITASLPAIVSVTDQTGEARYPAFKAIMGAKKKPITTWSLADLGVDAAAVGLG
                     AASVRTIDVAPTPARTAGTIITDDGEGAAQLADFLAGRGFI"
     gene            complement(3618..4565)
                     /locus_tag="PLK64_07095"
     CDS             complement(3618..4565)
                     /locus_tag="PLK64_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HPV79546.1"
                     /translation="MTSVLFDHPGPKARARHRIIAAVSALLVVLALWWALAQFKEKGN
                     LDAAKWTPFLTADIWVNYLLPGLQNTLIAAALSIVFAGAFGLVFGMGRLSHIPAIRWV
                     STVVVEFFRSVPVLVMMFFAFNLYAHNGVFRSDLNPLAGVVTGLTLYNGAVVAELLRS
                     GVGSLPKGQTEAGLAIGLSRGQTLRSVLLPQAITAMLPALVSQLVVILKDTALGYLIT
                     YQELLNKFQQIGSYKSNPVPALIVIAAIFIVINYALTSLASYLERRTRQRGRATIPKG
                     AIGPAPVDTQPIARALHDADEEVLHERYRAPGRTDDEHP"
     gene            complement(4562..5227)
                     /locus_tag="PLK64_07100"
     CDS             complement(4562..5227)
                     /locus_tag="PLK64_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HPV79547.1"
                     /translation="MGELFASYNILGAFGLTILLAVFSAIGSLLIGTVVAIMRVSPID
                     FLNKVGAFYVNIVRNTPLTLIIVFCNIGFIVNLGMSFTENIARNNIIWAIIGLSFYHA
                     AFVCEALRSGVNTVPVGQAEAARSIGLTFSQSLSEVLLPQAFRGAITPLGNTLIALTK
                     NTTVATAIGVAEISGLMKEMMEFRADLIIVIFLLVAIGFVILTLPIGLLTTRLSTKLA
                     VKR"
     gene            complement(5315..6031)
                     /locus_tag="PLK64_07105"
     CDS             complement(5315..6031)
                     /locus_tag="PLK64_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPV79548.1"
                     /translation="MKIGIKFDQPGLGLKSGADYTGMDVDLAKYLAKELSLGTPEFVE
                     AVSAQRETLIQTDQVKFIVGTYSITDARKEKVSFAGPYFIAGQDLLVRADDNSITGVE
                     SLNGKKLCSVKGSTSAANVQKAVPGVNLQEYGTYTLCVDALKAKNVDALTTDDTILAG
                     YAAQEANKGLMKVVGKTFSKESYGVGLKKGDTAFCQKVTDAINKYVSSGEWQKAVDKN
                     LGPAGYKAGAGNPPTPAPCS"
     gene            complement(6177..6905)
                     /locus_tag="PLK64_07110"
     CDS             complement(6177..6905)
                     /locus_tag="PLK64_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HPV79549.1"
                     /translation="MVVITGVNKHFGDLHVLRDIDLTVGRGEVVVVLGPSGSGKSTLC
                     RTINRLEAYESGSITIDGKTLPDEGKALAQLRADVGMVFQSFNLFAHKTILENVTLGP
                     MKVRKKSKAEAEAKARELLARVGVTTQADKYPAQLSGGQQQRVAIARSLAMEPKVMLF
                     DEPTSALDPEMVNEVLDVMTSLARSGMTMIVVTHEMGFARRAADRVVFMADGEIVEVA
                     TPATFFTSPRSNRAKDFLGKILSH"
     gene            complement(7215..7601)
                     /locus_tag="PLK64_07115"
     CDS             complement(7215..7601)
                     /locus_tag="PLK64_07115"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HPV79550.1"
                     /translation="MEEGSTGAGETLEVVSSVSYDAQGNVMTAEQVAAQAAAAAEAKA
                     ATDAAAAQAAAEAAAADAAAAAAASAAAAAAAAAPVERTYTVVKGDTLSAIGKKFGVN
                     WRDIASLNQVKNPDLIHPGQVFRIPN"
     gene            7723..8961
                     /locus_tag="PLK64_07120"
     CDS             7723..8961
                     /locus_tag="PLK64_07120"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPV79551.1"
                     /translation="MAIHRRIQAAVAAAAAIAIAACTTTAPTSGDAAGPPRSGGASAV
                     SGTAAYPSAGVSAPATSGAAERPPVVPAPGPAPASTPTAPAAPAPITIAFGGDIHFER
                     HLRPLLEGGDGLAELRPLLGAADITVVNLETSITTRGTPEPKSFTFRAPASALTTLAT
                     AGVDVAGMANNHAVDFGPVGLADTLAAKAASPIPVIGVGRTAAEAFAPAVFTVRGVSV
                     AVVAATQVPELTAAKFPATESRAGVAASIDNVRLLAAVRAARARYDVVVVFLHWGIER
                     TVCPDAAQVRTARALEQAGADIIVGGHQHRVLGSGWLGRAYVGYGLGNFVWWLKTASA
                     ADAATGVLTLSIDPAAVAARAAVPRDQWDGIAPIVVADDYAPLTISVVDGMPRASAQS
                     AKRLAAWEAARGCTTLKGRP"
     gene            9069..>10187
                     /locus_tag="PLK64_07125"
     CDS             9069..>10187
                     /locus_tag="PLK64_07125"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPV79552.1"
                     /translation="MCSPDLAAIVDIVVWVEPVDGVPTAHAANHLGRTRLHPGGRQEV
                     LEGVDPIASQDPMAFLPYEGPTGEVANPSPAQSRANAYPYAAERLLSFFADPDRSPDL
                     AIVHTPRHYFPDEGGHVGEHGSLDVIQSRAPLVLSGVGVRPLGVLDEHARLVDVGPTM
                     AVLAGVDREDLRDAQGEPLDGRPLTAYLTGETPRAVVGVLWDGAHCSDLLHLAEAGEL
                     PTVARLIERGLALRGGAVAEFPSITLTNHTSILTGVGPGRHGVMGNVYYDRGTGERVV
                     PNDEKTWHRSSEWIHDCVRTIFELVPGATASIDDAIDRGADYSTMGLVREDGSERGAG
                     GMGDHLPKASTSPFLSDPRFIQRDGYFRDAWDRPLIVLG"
BASE COUNT         1565 a         3273 c         3712 g         1637 t
ORIGIN      
        1 gcatcgcgag catctggagg tggagctcgt gccccatccg gcgggccggg tcaccggtgc
       61 aggactcggc ctcgccgagc accatgaact cgaccccagc ctcgtgcaac agggtggcga
      121 cggcgcgggc ggtgcgcttc gcggtgtcgt cgagagcgcc ggcgcagccg acccagtaga
      181 ggtactcgac gtcgtcgggg atcttgtcgc cctcgtagac gcgcaactcg aagtcgaggg
      241 tctcggccca ttccagccgg gccgaggcgc ccaggcccca cgggtcgccc gcgtgctcga
      301 ggttgcgcag catgatgccg gcctcctcgg ggaaggccga ctccatgagc acctggtagc
      361 ggcgcatgtc gacgatgtgg tcgacgtgct cgatgtcgac ggggcactgc tcgacgcagg
      421 cgccgcaggt cgtgcaggac cacagcatgt cgaaggggat gacgccctcg ccggtgatgc
      481 cgtgctcgcc gacgtgcgcg gtctcgtcgc cttcggggcc gatgagcgga cgggccgctt
      541 cggccgccgc agcctcgggt atgccgccct cgggcacctc tccgtcggcg ttcttcgccg
      601 ccagcagata gggggccttg gcgagcgagt ggtcacgcag ccccatgatg aggaccttgg
      661 gggacagcgg cttgtcggtg ttccaggcgg ggcactgact ctggcagcgg ccacactcgg
      721 tgcacgtcgc gaagtccagc aggcccttcc aggtgaagtc ctcgatcttg ccgacgccca
      781 tgacgtcgtc gtcgcccggg tcctcgaagt cgacctttgt gccctgccag tagaccggct
      841 ccagtgcccc gagagcccgg gggcggcgcg caaaggcgac gttggggatc gaggcgaaga
      901 tgtgcatgtg cttgctgtag gtgacgaaca ccgtgaagcc gagcagcacc gcgagtgaca
      961 ggagcaggaa gaaggtctcc cagaactcga cgttggtctt gggcaggatc gaggcgaacc
     1021 actgcgaggc gaacgccccg tgcaggaagc ccatcttgtc ggcgttggcc ggctcgtgga
     1081 gctcctgggc gttgatcgag gctccgcggt aggccaacag ggtccacacg acgttgaaga
     1141 tcatgaacag gacgaaccac gccgggccga tgtgcgagcc gaagaagcgg ctgcgccggc
     1201 cgaggttctt cggggcctgc tggatgcgca gcaccgtgaa cacggccagc gccgccacac
     1261 acagcagggc gaagaggtcc tcgaagaacc cgaaccaggc gaagttgccg acgatcggga
     1321 tctggaagtc gggcttgacc aacccgatga aggcttcgac gatcgtgaac aacaggacca
     1381 tgaagcccca gaacacgagg aagtgcgcgg cgcccggcac ggtccagcgc aggagtttgc
     1441 gctggaaggc gacctcagcg acctccgccc gcaccgccga gccggggtcg acgcgccgct
     1501 cagcctccag cggctggaac gtcttgccga cgcgatagag gaagtacgcc cggcgcccgg
     1561 caatcccgag gcagacgacg gtcatgagca gaccgaggac gaaacggacc cacatcggga
     1621 atcgagctcc ttcgtgccgt ctggacggga gaggtacggc gccgggtcag cccttgcgct
     1681 tccctacctc gtcgatgaga gccggcaaga ccttgtgcag gtcaccgacg acgccgaagt
     1741 cggcgagctg gaagaggggg gccttggggt ccttgttgac cacgacgatg gtcttgctgc
     1801 tctgcatgcc ggcgcggtgc tggatggcac cggagatgcc ggcggcgagg tagagcgacg
     1861 gcgccacggt cttgccggtc tggcccacct gcaactcgtg cggtgcccag tgctggtcgg
     1921 tgacggtgcg cgatgctccg acggctgcgc cgagcgcgtc cgcggtctgg ccgataacgg
     1981 cgaagccttc ctcggagccg acgccacgcc caccggcgac gacaactgcc gcgtcggtga
     2041 gctcggggcg accggtggac accttgggct cgcgggaggt gatcttggcg cccttggcgg
     2101 cctcgctcac ggcgacgtcg accgtctcga ccgacgggga cgccgcagcg gcctcggccg
     2161 acgtggcgtt gggccgcatg gtgatgacgg ccgggccgcg aacgatctgg ctgcgggcct
     2221 gccagcggcc ggcgaagacg gtctgcgtcg cgacgacgtt cccgccgtcg agggccacga
     2281 cagtggcgtc gggcacgatg cctgagttga gccggaccgc gacccgagcc gcgacgtcct
     2341 tgccctcggg gccgctggtc acgaggatcg cgaccgggtt ggcggtgccg gcgatctgga
     2401 cgacggcctc ggccttgggc agcgacaggt agtcgcccac ctcggccgcg ttcacggcat
     2461 acaccttggt ggcgccgtat tcgccgagct gcgcgacgac ggcgtcagag gcctcgccga
     2521 agacgacggc ggcgggctcg ccaagcgcac gggcggcggt gagcagttcg agggtgggct
     2581 tgcggacgcc ggccgcggtg gtctcggcga taacgaggac ttccgacacg atgagtctcc
     2641 tggattggta tgccgcggcg ccgcggcggt ctgtcgggtg gggtcggcga cgtggtcgcc
     2701 ggaaggtcag atgaagccgc gaccggcgag gaagtcggcg agctgggcag cgccctcacc
     2761 gtcatcggtg atgatcgtgc cggcggtgcg cgcgggtgtc ggggcgacgt cgatggtgcg
     2821 cacgctcgcg gcgcccagac cgacggcagc cgcgtcgacc ccgaggtcgg ccagcgacca
     2881 ggtggtgatc ggcttcttct tggcacccat gatcgccttg aaggcggggt agcgggcctc
     2941 gcccgtctgg tcggtcaccg agacgatggc cggcagcgac gcggtgatcc gctcgctggc
     3001 cacgtcggag tcacggcgga tcgtcacggc cccgccctcg acggacaact cgccggcgaa
     3061 ggtggcctgg ttgatgccca ggcggtcggc gaccatcgac gggaccaccg agagctcggc
     3121 gtcggtcgaa gccatgccgg tgaggacgag gtcgaagcct tccttctcca gcgccttggc
     3181 cagcaccagc gaggtgctca gggcgcacga tccgtggatc gcgtcgtcga cgacgtgcac
     3241 tgccttgtcg cccccgatgg ccagcgcctt gcgcagacct tcgctggcct tggcgggccc
     3301 gacggtgagg aagacgacct cggaggcgac gcccttctca gccaactggg cggcctgctc
     3361 gacggcatac tcgtcaagct cggacaggcg cccttcgacg ccggcgcggt cgacggtgag
     3421 atctggggcg aacttccagg tggccgtggg ctccggcacg tacttgacga ggacgacgat
     3481 cttcatgcgt gatgacctgc cttagggtgc ggtgcggcgt tcgtgtttga gcctgccaga
     3541 gatgctcgaa tcgggaaggg ggtcataggt cccttaccga cgagtagcag gagtttcggg
     3601 tgccgccgag cgtggaccta agggtgctcg tcgtccgtcc gccccggggc gcggtagcgc
     3661 tcgtggagga cttcttcgtc ggcgtcgtgc agggcgcggg cgatcggctg ggtgtccacg
     3721 ggggcggggc cgatcgcgcc cttcgggatg gttgcgcggc cacgttggcg ggttcgccgc
     3781 tcgaggtagg aggccagcga ggtgagggcg tagttgatga cgatgaagat ggctgcgatg
     3841 acgatgaggg ccggcaccgg gttggacttg taggacccga tctgctggaa cttgttgagc
     3901 agttcttggt aggtgatgag gtagccgagg gcggtgtcct tgaggatgac gaccaactgg
     3961 ctgaccaacg ccggcaacat cgccgtgatc gcctggggca acaggacgct gcgcaaggtc
     4021 tggccgcggc tgaggccgat cgcgagcccg gcctcggtct gccccttggg caggctgccg
     4081 acgccggagc gcagcaactc ggcgacgacc gctccgttgt agagggtgag gccggtgacg
     4141 acgccggcca gcgggttgag gtcgctgcgg aagaccccgt tgtgcgcgta gaggttgaag
     4201 gcgaagaaca tcatcacgag caccggcacc gaccggaaga actcgacgac caccgtgctc
     4261 acccagcgga tcgccggaat gtgggacagg cggcccatcc cgaagaccaa cccgaaggct
     4321 ccggcgaaga cgatcgagag cgcggccgcg atgagggtgt tctgcaggcc cggcaggagg
     4381 tagttgaccc aaatgtcagc cgtgaggaac ggggtccact tggccgcgtc gaggttgccc
     4441 ttctccttga actgcgccag cgcccaccag agcgccagaa cgaccaagag ggcactgaca
     4501 gcagcgatga tccggtgccg ggcccgggcc ttggggccgg ggtggtcgaa gaggaccgag
     4561 gtcatcgctt caccgccaac ttggtcgaca ggcgagtcgt caacaggccg atcggcaggg
     4621 tgaggatgac gaaaccgatg gcgacgagaa ggaagatgac gatgatcagg tcggcgcgga
     4681 actccatcat ttccttcatc aggccgctga tctcggccac gccgatggcg gtggcgacgg
     4741 tggtgttctt cgtcagggcg atgagggtgt tgcccagcgg cgtgatcgcc ccgcggaacg
     4801 cttgtgggag caggacttcg ctcaggctct gcgagaaggt cagcccgatc gatcgggcgg
     4861 cctcggcctg gccaaccggc acggtgttga cgccgctgcg cagggcctcg cagacgaagg
     4921 ctgcgtggta gaacgacagc ccgatgatcg cccagatgat gttgttccgg gcaatgttct
     4981 cggtgaacga catgccgagg ttgacgatga agccgatgtt gcagaacacg atgatgaggg
     5041 ttagcggcgt gttgcggacg atgttgacgt agaacgcgcc gaccttgttg aggaagtcga
     5101 tgggggagac ccgcatgatc gcgacgacgg tgccgatgag cagcgatccg atggcggaga
     5161 acacggcgag caggatggtc agcccgaaag ccccgaggat gttgtagctg gcgaacagct
     5221 cacccatcgc gacgctcctt cctcgtgagt gaggagacgg ggtatgccgc ccgcgctcgc
     5281 tgcgcgggcg gcataccggc gtctgtggat gggatcagga gcagggggcg ggggtcggcg
     5341 ggttgcccgc gccggccttg taacccgccg ggccgaggtt cttgtcgacg gccttctgcc
     5401 actcaccact ggagacgtac ttgttgatgg cgtcggtgac cttctggcag aaggcggtgt
     5461 cgcccttctt gaggccgaca ccgtaactct ccttggagaa ggtcttgccg acgaccttca
     5521 tgaggccctt gttggcttcc tgcgcggcat acccggcgag gatggtgtcg tcggttgtca
     5581 gggcgtcgac gttcttggcc ttgagcgcgt ccacacagag cgtgtaggtg ccgtactcct
     5641 gcaggttgac gccggggacg gccttctgga cgttggcggc cgaggtcgac cccttgaccg
     5701 agcagagctt cttgccgttg agcgactcga ccccggtgat ggagttgtca tcggcgcgga
     5761 ccagcaggtc ctggccggcg atgaagtagg ggccggcgaa ggagaccttc tccttgcggg
     5821 cgtcggtgat ggagtaggtg ccgacgatga acttcacctg gtcggtctgg atcagcgtct
     5881 cgcgctgtgc cgacaccgcc tcgacgaact cgggtgtccc caggctgagt tccttggcga
     5941 ggtacttggc caggtcgacg tccatgccgg tgtagtcggc cccgctcttg agcccgagtc
     6001 ccggctggtc gaacttgatg ccgatcttca cggtgccgcc cgaggcggtg cttgcgccgc
     6061 cgccgcaggc ggcaagcccg agggccaccc cacggcgatc gtggcgacac tgatgcgtcc
     6121 gagcttcatt gcgtcctcct gaagtgatct atcggatcga gcagcacacg gtctgctcag
     6181 tggctgagga tcttcccgag gaagtccttg gcccggttgg agcggggaga ggtgaagaag
     6241 gtggcggggg tggcgacctc gacgatctcg ccgtcggcca tgaagaccac ccggtcggcc
     6301 gctcggcgcg cgaaccccat ctcgtgggtg acgacgatca tcgtcattcc ggatctggcc
     6361 aggctcgtca tcacgtcgag cacctcgttg accatctcgg ggtccagggc cgaggtgggc
     6421 tcgtcgaaga gcatgacctt gggctccatc gccaggctcc gggcgatggc gacccgctgc
     6481 tgctgaccgc cggacagctg ggccgggtat ttgtcggcct gggtggtgac cccgacccgc
     6541 gcgagcaact cgcgggcctt ggcctcggcc tcggccttgc tcttcttgcg gaccttcatc
     6601 gggcccagcg tgacgttctc gaggatcgtc ttgtgcgcga agaggttgaa ggactggaag
     6661 accatcccca catcggcgcg cagttgcgcc agggccttgc cctcgtcggg aagggtcttg
     6721 ccgtcgatgg tgatcgaccc ggactcatac gcttcgagcc ggttgatcgt ccggcagagg
     6781 gtggacttgc ccgaccccga ggggccgagg acgacgacga cctcgccccg accgaccgtg
     6841 aggtcgatgt cgcgcagcac atggaggtcg ccgaagtgtt tgttgacccc cgtgatgacg
     6901 accatcggct ccttggtgga cgcggcggcg agtgggggtt cgtccccctc tgctggccgt
     6961 ccggtgggat tctggtcccg cgctgcctcg gtcattcgct caacctagcg ccccaccacg
     7021 catttgggca ggggacgtcg agggctttcc gaattgccgt cacctgggca acacaagcgc
     7081 attcccacag aaacaaaaag gcaaaacacc gccgagtatg ctgcgcggcc ggtgcggact
     7141 tccggacgca gacagggggc ctcccggttc gggaggcccc ctgcgtggtg gtgcggagcc
     7201 ggcgctgact gcggtcagtt ggggatgcgg aagacctgtc cggggtggat caggtcgggg
     7261 ttcttgacct ggttgagcga ggcgatgtcg cgccagttca cgccgaactt cttgccgatc
     7321 gccgacaggg tgtccccctt gacgacggtg taggtccgct cgaccggtgc agcggcggcc
     7381 gcagcggcgg cggcgctcgc agcagcggca gcagccgcgt ctgcggcggc ggcctctgcg
     7441 gcagcctggg ctgctgcggc atctgtcgca gccttggcct cggctgcggc ggcagcctgg
     7501 gcggcgacct gctcggccgt catgacgttg ccctgggcgt catagctgac cgaggaaacg
     7561 acctccaggg tctcgccggc gccggtgctg ccttcttcca tgagtgccac gggtggctcc
     7621 ttctcctgtt tcctagccgg gccgagaagg cccggcgcca cgagtccccc cgtgggtgca
     7681 gtgagcctag cctgcaggct ccgtaggaca gactgatctg ccatggccat ccaccgtcgg
     7741 atccaggccg ccgtggccgc cgccgctgcg atcgccatcg cggcgtgcac gacgaccgcg
     7801 cccacgtcgg gtgacgcggc cggcccaccg agatcgggag gggcgagcgc ggtgtcgggg
     7861 acggcggcat acccgagcgc gggagtgtcg gcaccggcca cgagcggcgc cgccgagcga
     7921 ccgccggtgg tcccggcccc gggtccggca ccggcatcca ctccgacggc accggcggcg
     7981 cctgcgccga tcaccatcgc gtttgggggt gatattcact tcgagcgaca cctgcggccc
     8041 ttgctcgagg gtggggacgg gcttgccgaa ctgcgtccgc tgctgggcgc ggcggacatc
     8101 accgtcgtca acctcgaaac ctcgatcacc acccgcggga cgccggagcc caagtcgttc
     8161 accttccggg cgccggcctc ggcactgacg acgctcgcga ccgccggcgt cgacgtggcg
     8221 gggatggcca acaaccacgc cgtcgacttc gggccggtgg ggctggccga cacgctggcg
     8281 gccaaggcgg ccagcccgat ccccgtgatc ggcgtcgggc gcacggctgc cgaagccttc
     8341 gccccggccg tcttcaccgt ccgcggcgtg tccgtggcgg tcgtcgcggc gacccaggtc
     8401 cccgagctca ccgcagccaa gttccccgcg accgaatcca gggcaggggt ggcggccagc
     8461 atcgacaacg tgcggctcct cgcggccgtg cgcgcggcgc gggcccgtta cgacgtggtc
     8521 gtcgtcttcc tgcactgggg catcgagcgg accgtatgcc ccgacgcggc ccaggtgcgg
     8581 acggcccgcg ccctggagca ggccggggcc gacatcatcg tcggagggca ccaacaccgg
     8641 gtgctgggct ccggctggct gggtcgggcc tacgtcggct acgggctggg gaacttcgtc
     8701 tggtggctca agaccgcgtc cgcggccgac gccgcgaccg gtgtcctcac cctgtccatc
     8761 gaccccgcag cggtggcggc gcgcgccgcg gtgccgcggg atcaatggga cggaattgcc
     8821 cccattgtcg tcgcggacga ctacgctccg ttaactatct cggtcgtgga cggcatgcca
     8881 cgggcttccg cccagagcgc taagcggctg gccgcatggg aggcggctcg gggttgcacg
     8941 acgttgaagg ggagaccatg agcatgaggc cggcccagga cggggaccgg actggggatc
     9001 cgactgggga tccgactggg gatccgactg gggaccggac aggggatcgg gaccggatga
     9061 tccgggcttt gtgttcgccc gacctcgctg cgatcgtcga catcgttgtt tgggtcgagc
     9121 cggtggatgg agtgcccacc gcgcatgcgg ccaaccatct cgggcggacc aggctgcatc
     9181 ccggtggtcg tcaggaggtc cttgagggag tggaccccat cgccagccag gacccgatgg
     9241 ccttcctccc ctatgagggc ccgacgggcg aggtggccaa ccccagcccg gcccagtccc
     9301 gggccaacgc ctatccgtat gccgctgaac ggctgctgtc cttcttcgcc gacccggatc
     9361 gcagccccga cctggcgatt gtccacactc ctcggcacta cttccccgac gagggtgggc
     9421 atgtcgggga gcacgggtcc ctcgatgtca tccagtctcg tgcgccgttg gtgctctcgg
     9481 gtgtcggtgt ccgcccgctc ggcgtgctcg acgagcacgc ccggctggtc gatgttggcc
     9541 cgacgatggc ggtcctcgcg ggggtcgacc gggaagacct gcgcgacgcc cagggtgagc
     9601 cgctcgacgg acggccgctc acggcatacc tcacggggga gactccgcgc gcggtggtgg
     9661 gcgtcctctg ggacggggcg cactgttcgg acctcctgca cctggccgag gccggcgagt
     9721 tgccgacggt ggcgcggctc atcgagcgcg ggctggcgct gcgcggggga gcggtggcgg
     9781 agttcccgtc gatcacgctg acgaaccaca cctcgatcct cacgggtgtc gggccgggtc
     9841 ggcatggggt gatgggcaac gtctactacg accgcgggac cggcgagcgg gtcgtgccca
     9901 acgacgagaa gacctggcac cgatcctccg aatggatcca tgactgcgtg cggacgatct
     9961 tcgagctggt ccctggagcc accgcctcga tcgacgacgc gatcgaccgg ggcgcggact
    10021 actcgacgat ggggctcgtc cgcgaggacg ggtcggagcg gggcgccgga ggcatgggcg
    10081 accacctgcc gaaggcgtcg acctcgccgt tcttgtcaga cccgcggttc atccagcgcg
    10141 acggctactt ccgggacgcc tgggacaggc ccttgattgt actcggt
//
LOCUS       DAOMZY010000097        10165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_6232_length_10165_cov_5.352819, whole genome shotgun sequence.
ACCESSION   DAOMZY010000097 DAOMZY010000000
VERSION     DAOMZY010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 10165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10165
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6232_length_10165_cov_5.352819"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..358
                     /locus_tag="PLK64_07130"
     CDS             158..358
                     /locus_tag="PLK64_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79553.1"
                     /translation="MVLLAAADPQVGTDVLSGYVSFFVDAARLPDQPGLGPVQEHRRK
                     RAAYTMPLLRKRIEAETTDARF"
     gene            414..1025
                     /locus_tag="PLK64_07135"
     CDS             414..1025
                     /locus_tag="PLK64_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPV79554.1"
                     /translation="MRSNAPSLMPVFRSRHQADLLTLLSLHPDQDYTVTDLAARLGIP
                     LTTAHREAQRLEESGLLTGRAVGRSRLLRANTSHRAFEPLARLLLVTFGPHVVVAEEF
                     AALPGVEQVVIFGSWAARYAGVPGVAPADIDVLVVGNPPRAEVYAAAERAEARLGMPV
                     NPTIRASSRWASGDDPLVATIQGGPHLDVTAEMPPVDKPAHAT"
     gene            1055..1516
                     /locus_tag="PLK64_07140"
     CDS             1055..1516
                     /locus_tag="PLK64_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79555.1"
                     /translation="MSGWQVGAAEIDELLADRSLQQVTGTAADGGPGLARAHQTFTSA
                     QVLLANDPANAFVLAYDAARQACSALLAHQGLRPTAGGGHIAVERAVRAQFGAVFVAY
                     GGLRRRRNEVEYPSHPDEAVAHDEAAAALDAVEAIMSAAEQLLPHLTLFRA"
     gene            1632..2834
                     /locus_tag="PLK64_07145"
     CDS             1632..2834
                     /locus_tag="PLK64_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPV79556.1"
                     /translation="MDDLGDAAVALLSDLVAIDSVNPGLVDDAAGESAIVAFLANRLR
                     ANGFTVTIINAEGHANRPSLLAVGPGPADAPTVVLNGHLDTVGVSGMPSPFTPRVEGH
                     QLFGRGAADMKGGVAALIVAAERLATDRLNADRLTPYQPTHEGPTLEQPTGARGGIRP
                     VLALVTDEEDASLGSEAVIAALAALNIHPDVCLIGEPTDLTPCHTLRGFGVARVSFQG
                     RAAHSSQPHLGINALTQLGRFLHAVDQRAPHLHAEGSDLMVTVARGGTSAFVIPDSAE
                     CLVEIRTSPDGRGTTVLDEVQALLDPVWDATVELVAARDGWRLDDSGPAADLATQLAA
                     ALTTGATFDAPYWMEAPLWQAVCPTLICGPSGGSLHAVDEWVDLRQVRAFTRALVDVL
                     SSWSPDAD"
     gene            2824..4101
                     /locus_tag="PLK64_07150"
     CDS             2824..4101
                     /locus_tag="PLK64_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPV79557.1"
                     /translation="MPTDPRGLRSDAPLFDAWLTFHEQALTPFTIPGHKQRHDLVGDV
                     VAGDVPLYAGLDTMKLTTGVLLDAEARAAQLWGADVCRFSTGGATHANQALALAVAGD
                     GDQVVVSRTLHRSMLLGLVLAGLTPVWVRPEVDAATGLPLGVAPATVAAALAEHPNAR
                     AVFVGDPSYVGTVGDVAGLADAAHHHGVPLIVDAAWAAHFGFHPDLPAHPMALGADAM
                     VTSAHKTLPAWSQAAIILANTERIDASRLDAAVEATATTSPAGAILASIDASRALLAR
                     DGEALLGTAIAATRAARDHLRQVPGLTVVDGPHVDPAKLILVLSGTGADGNAIEQDLL
                     LAGMPVESADRDVLIAIVSLADTVEGVTRLADAIAASVERHRSAPRPVVGAASYSVDP
                     VTVMPPRQAFFSAATPVPVEDAVGRVSAELIAP"
     gene            complement(4896..5720)
                     /locus_tag="PLK64_07155"
     CDS             complement(4896..5720)
                     /locus_tag="PLK64_07155"
                     /inference="COORDINATES: protein motif:HMM:NF024783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HPV79558.1"
                     /translation="MDALVQHFIYPLSHAGGYVFEGGRQITPDNYWASSLVGKTDEWG
                     MSTGYRLIEPGDWVWAYFSQPIAQILGVGRVVKPVTWHARWGRYSVHIRWDRALTSAL
                     KKKPIDYAEYQQQVQGAAVRANKSTVQVLEGWLLDHGSRWVSEARAVKFAQREVLQRL
                     GQAEFRARVLKAYGGTCAITGTTEEGVLQAAHILPIGNGGRHTVANSLLLRADVHNLF
                     DLGLLTITPGMKVAVDPTVTDGIYRGLVGRPVSVPRAVNRQELKKALTEHRSRRNK"
     gene            complement(5814..6653)
                     /gene="phaZ"
                     /locus_tag="PLK64_07160"
     CDS             complement(5814..6653)
                     /gene="phaZ"
                     /locus_tag="PLK64_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0006112 - energy reserve metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly(3-hydroxyalkanoate) depolymerase"
                     /protein_id="HPV79559.1"
                     /translation="MPLSGVTGARTVTVRGHELRIWTRPGAPGRTPLVLCSGIGTGYS
                     LLLPFVEALDPDVPVIGFDAPGVGGSPIPRLPYRFPILASLIGALVRQLGYDRFDVLG
                     ISWGGGLAQQIAFQNPRRCRRVVLVATGTGSLMVPARPNVLLTMATPRRHRDPEYLMA
                     VAPSIYGGQVRNHPDLVASLAHARSRGGVSRRGYAYQMLAGAGWTSLPLLPLLRQPVL
                     ILAGDDDPIIPLANAWLLKSLIPHSSLYVYHDGHLGLVTSASVLGPVVGDFLGSAHGL
                     LEE"
     gene            complement(6670..8373)
                     /locus_tag="PLK64_07165"
     CDS             complement(6670..8373)
                     /locus_tag="PLK64_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPV79560.1"
                     /translation="MPPTVTVDPAEGTDDLAVPLDHLLVEAALGPLRRFAPDLSTARL
                     LGSLASRPVSTGRRVGALAADLAAIVAGKSTVAPDRRDRRFADEAWAKNPLLRRLVQS
                     YLASGATAAQLVSDAGLGWRDAQRVEFLLDNLTEALSPSNLPLVNPASAKAAIDTGGA
                     NFARGMSAFAKDMSAKPRVPQMVDSSGFELGRNIAVSPGAVIARTEVFELIRYQPSTP
                     KVHEVPLLIVPPTINKFYAMDLAPGRSLVEFLVGQGQQVFIISWRNPLAHHADWDSDT
                     YAAEIIKAMDITAKAAGVEKVALTGICSGGILSSLAVAHLAATDRLDRLAAFSLFVTV
                     LDQEHAGMPAALAGPRTTDLAKRASRQRGFIDGRDLAEAFAWLRPGDLVWNYWVNNYL
                     LGRRPPAFDILFWNADTVRMPAGLHADFIDIAMENRLVHAGTQTTLGTPVDLGEITTD
                     AYVVAGIADHITPWQNCYQSTQLLGGDTRFILSTSGHIAALVNPPNNPKASYRDGSTS
                     YTDANEWLENAEQHPGAWWPDYAAWLAARSGALVKAPTRLDGGGTSVLAQAPGTYVLE
                     P"
     gene            complement(8380..9864)
                     /locus_tag="PLK64_07170"
     CDS             complement(8380..9864)
                     /locus_tag="PLK64_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPV79561.1"
                     /translation="MNLAQAFVDSAAKHADRIALRTASGATTTYAELDNLTARAAGLL
                     AEAGVGVGDRVGLMLPNVPEFAVVYYGVLRAGAIVVPMNPLLKGREVAHYLDDSGANL
                     IFTGAMSADEVHAGAPEGCTVIVVDETFAPRLAAATPVTEVPERGSDDTAVILYTSGT
                     TGLPKGAELTHKNLLSNAGLMRTDLISMDKDDVVFGGLPLFHVFGQTCSLNAALLCGA
                     SIVLLPRFDAATAQALILDNNITVVEAVPTMYSALAEGSPIEAPHLRVAVSGGAALPV
                     EVLARFEERWGCTVLEGYGLSETSPAASFNHMGRPRKVGSVGEPVSGVEITVFDEDGK
                     ELPIGEVGEIAIRGDNVMKGYWNAPEATAKALRPDGWFLTGDMGRKDEDGFVFIVDRK
                     KDLIIRGGYNVYPREIEEVFYEHPDVLEVAVIGIAHTTHGEEVGAAVVVRAGSTVTAD
                     DLRTYLRERVAAYKYPRVVWLLDELPKGPTGKILKRMITAPEKS"
BASE COUNT         1714 a         3587 c         3240 g         1624 t
ORIGIN      
        1 gagcgtccga ctgaaaatcg gaaggtcggc ggttcgaccc cgcccctggc caccaccact
       61 gcgctgcccc acccgagggt atgccgcgtg accggatata cggttgccgc cggattgctt
      121 ccccgcgaga ctcgccgacc atgagccatc gtccgccgtg gtcctccttg ccgccgctga
      181 tccacaggtc ggcaccgatg tcctcagtgg ctacgtgtcg ttcttcgtcg atgcggcgcg
      241 cctgccggac cagcccgggc tgggaccggt ccaggagcac cgacgcaagc gggcggcata
      301 cacaatgccg ttgttgcgca agcgaatcga ggcggaaaca accgacgcgc gcttctgatc
      361 cgggggcgcg cgaggtattg atgctacgca tagacggatg ctatcttccg tttatgcgta
      421 gtaatgcccc ctcgctgatg ccggtgttcc ggtctcggca tcaggccgat ctgctcaccc
      481 tgctctccct gcaccccgat caggactaca ccgtcacgga cctggcagcc cggctcggaa
      541 tcccgttgac caccgcccat cgcgaggctc agcgcctaga ggagtccggt ttgctcactg
      601 ggcgtgcagt cggtcgatca cgcctcctgc gggccaatac gtcgcaccgc gccttcgaac
      661 cgctggctcg acttctgttg gtcaccttcg ggccgcacgt cgtcgtggcc gaggagttcg
      721 ccgccctccc cggcgtcgag caggtcgtga tcttcgggtc gtgggccgct cgctatgccg
      781 gcgtccctgg agtcgcgccc gcggacatcg acgtcctggt ggtcggcaac ccaccccgcg
      841 ctgaggtcta cgcggcggcc gagcgggcgg aagcccgact cggtatgccg gtcaacccga
      901 ccatccgggc ctcaagccga tgggccagcg gcgacgaccc actcgtggca acgatccaag
      961 gcggcccaca cctggatgtc actgccgaaa tgcctcctgt ggataaacca gctcacgcga
     1021 cctgacgcac cgcatcgccc agtacggtgc ctgaatgagt ggatggcagg tgggtgcagc
     1081 cgagatcgac gagttgctag ccgaccgttc gctacagcag gtcaccggca ctgccgcaga
     1141 cggaggcccc ggtctggctc gggcgcatca gacattcaca tcggcgcaag tcctgctcgc
     1201 caatgaccct gccaatgcgt tcgtgctggc ctatgacgcc gctcggcaag cctgctcagc
     1261 cctgcttgcc caccaagggt tgcgccccac ggcaggcgga ggccacattg cggttgaacg
     1321 ggcagttcgc gcccagttcg gtgcggtgtt cgtcgcctac ggagggttac gtcgacgccg
     1381 aaacgaggtc gaatatccat cccatccaga cgaggccgtc gcgcacgacg aagccgcggc
     1441 tgctctggac gccgtcgagg cgatcatgag tgccgctgag caactactcc cgcacctcac
     1501 cctgtttcgg gcctgatcct cgccacgcgc ccggccctgc ctaaccacac tggacggtgc
     1561 tgacgtttca taccagtgtc ggagtccgtg agcacacggg cggtcagccc tactcccctt
     1621 acgcttcgcg gatggatgat ctcggcgacg cggcggttgc gctcctgtcg gacctcgtcg
     1681 cgatcgacag cgtgaaccct ggcctggtcg acgatgctgc gggcgagtcg gccatcgtcg
     1741 ccttcctcgc aaaccggctg cgcgccaacg gtttcaccgt caccatcatc aacgccgaag
     1801 gccacgcaaa ccgcccgagc cttctggcag tcggccccgg gccagccgac gcacccaccg
     1861 ttgtcctcaa cggccacctc gacacggtcg gtgtctccgg tatgccgtcc cccttcaccc
     1921 cccgcgtcga aggccaccag ctcttcggcc ggggcgcagc cgacatgaag ggcggtgtcg
     1981 ccgcgctcat cgtcgccgcc gagcggctcg ccactgatcg cctaaatgcc gatcgcctga
     2041 ccccctatca gcccacccac gaaggcccaa ccctcgaaca accaacaggc gcccgcggcg
     2101 gcatacgccc cgtcctagcg ctggtcaccg acgaggagga cgcgagcctg ggcagcgaag
     2161 cggtcatcgc ggccctcgcc gccctcaaca ttcacccgga cgtctgcctc atcggtgagc
     2221 cgaccgacct gaccccgtgc cacacgctgc gcggcttcgg cgtggcccgc gtcagtttcc
     2281 aaggccgcgc ggcgcacagc tcgcaacccc atctgggcat caacgcactc acccaactcg
     2341 gccggttcct gcacgccgtg gaccagcgcg ccccccacct gcacgccgaa ggcagcgacc
     2401 tcatggtgac cgtcgcacgc ggtggcacct cggcattcgt catccccgac tccgccgagt
     2461 gcctcgtcga gatccgcacg agccccgatg gccgcggcac caccgtcctc gatgaggtcc
     2521 aggccctcct cgaccccgtc tgggacgcca ccgtcgagct cgtcgccgca cgagacggct
     2581 ggcgcctcga cgactcgggg cccgccgccg acctcgcgac ccagctcgcc gccgcactca
     2641 ccaccggcgc gaccttcgac gccccctact ggatggaagc cccactctgg caagccgttt
     2701 gcccgaccct gatctgcggc ccgtccggcg gcagcctgca cgccgttgac gaatgggtcg
     2761 acctgcgtca ggtccgcgcg ttcacccgcg cccttgtcga cgtcctgtcc tcctggagcc
     2821 ccgatgccga ctgacccgcg cggactgcgt tctgacgcac cgctgttcga cgcgtggctg
     2881 acgttccacg agcaggccct gaccccgttc accatccccg gccacaaaca gcgccacgac
     2941 ctcgtcggcg acgtcgtcgc cggcgacgtc cccctctacg cgggcctgga cacgatgaag
     3001 ctcaccaccg gagtcctgct cgacgcggag gctcgcgcgg cgcaactctg gggcgccgac
     3061 gtatgccgtt tctccaccgg cggcgcgacc cacgccaacc aggccctcgc cctggcagtg
     3121 gccggcgacg gcgaccaggt cgtcgtcagc cgcaccctgc accgctcgat gctgctcggc
     3181 ctggtcctcg ccgggctcac cccggtctgg gttcgccccg aggtcgacgc cgccaccggc
     3241 ctgccgctag gcgtcgcacc cgcgaccgtt gccgccgccc tcgccgaaca cccgaacgcc
     3301 agagccgttt tcgtcggcga tccctcctat gtcggcaccg tcggtgacgt ggctgggctc
     3361 gcggacgccg cgcatcacca cggcgtccca ctcatcgtcg acgccgcctg ggcggcccac
     3421 ttcggcttcc accccgacct gcccgcgcac cccatggcgc tgggcgccga cgcgatggtg
     3481 accagcgccc acaagaccct gcccgcctgg agccaggccg cgatcatcct ggccaatacg
     3541 gagcgcatcg acgcctcgcg cctcgacgcc gctgtcgaag cgaccgcaac caccagcccc
     3601 gcaggcgcga tcctcgccag catcgacgcc tcccgcgcct tgctcgcccg cgacggtgag
     3661 gcgctgctcg gcaccgccat cgccgccact cgggccgcgc gcgaccacct gcgtcaggtc
     3721 ccaggcctca ccgtcgtcga cggccctcac gtcgaccccg cgaagctcat cctcgtcctg
     3781 tccggcaccg gcgccgacgg caacgcgatc gagcaggacc tcctgctcgc aggtatgccg
     3841 gtcgagtcgg ccgaccgcga cgtcctcatc gcgatcgtct ccctcgccga caccgtggag
     3901 ggagtcacgc ggctcgccga tgccatcgcg gcctccgtcg aacgtcaccg cagcgcccca
     3961 cgccccgtgg tgggcgccgc gtcctactcc gtcgacccgg tgaccgtcat gccaccccgc
     4021 caggcgttct tctccgccgc caccccggtg ccggtcgagg acgccgtcgg ccgcgtgagc
     4081 gcagaactga tcgcccccta gccacccggc atacctgtcc ttgcccctag tgaggagatc
     4141 acggcagccg ccctcagtgc gctccttgcg gcccgggacg ccggcgtccg catcgcgtat
     4201 gccgccgacc ccaccctgcg caccctgcgc gtcactgccc cttaggaaca acaactacgg
     4261 aagcgtgtgg cggccccttg aaccgattcg actcgtcgtt agggactcgg gaaggcctta
     4321 cgaagtacgt ctatcaactg acgagcagtc cgctcgtcaa gttggaggga ctggctgctc
     4381 ttaggcctac tcacccgctc ctccgagccg aaggtggtca aatggacgaa cttctcacct
     4441 gaggcactca cgatcgattg gtagaagcag tccacctccg atgggtgtac tcgcacatct
     4501 tgcgcccctt gttgaagcga cctcactctg gccatctcgc acctccgaac cttgttcccc
     4561 gttggcctgg actcacaata gatgctggga tgtccgattc cgaggttggt aacaccagca
     4621 tcccttcacg atcttgcccc cggcacgaag atcaactctc tggccagccc aacccgctct
     4681 gcgaggatta acctcgccac ccaccagcgt gagcgccaac gccgacgcca agtccgggca
     4741 ccgaccggtc gccaggcaga ctcatcatgc gcttccgctc agggtgtctg ggccctcgca
     4801 ttcacatgag cccggataca gcgcaggcac tcttggtgtg gccagagaaa gtatcacaaa
     4861 tgtatctcgt aggttgagga ccaatgtcga accaactact tgttgcgtcg gctccgatgt
     4921 tctgtcagtg ccttcttcag ttcttgccta ttcaccgcgc gtggcacaga tacgggtcgc
     4981 cctacaagtc cacgatatat cccgtcagtc accgtcgggt cgaccgcaac cttcatcccg
     5041 ggagtaatcg taagaagtcc gagatcaaac aggttatgaa catcagcacg caagaggaga
     5101 gagttcgcca ccgtatgtcg accaccgttt ccgatgggaa ggatgtgagc tgcctgcagc
     5161 accccttctt cggtggtccc ggttatcgcg caagtcccgc cgtacgcctt caagacgcga
     5221 gcacgaaact ccgcttggcc gagccgctgg agcacttcgc gctgggcgaa tttgacggcc
     5281 cgagcttccg atacccaccg agagccgtga tccagaagcc atccctccag cacttgaaca
     5341 gttgatttgt tggcacgaac ggccgccccc tgtacctgct gttgatactc tgcgtaatcg
     5401 ataggcttct tcttcagagc tgaggtgagc gctcggtccc agcgaatatg aacggaatag
     5461 cgcccccagc gggcgtgcca agttacaggc ttgacaaccc tgccgacgcc gagaatctgt
     5521 gcgatgggtt ggctgaaata ggcccagacc caatcgcctg gctcgatcag ccggtagccg
     5581 gtcgacattc cccactcgtc agtcttgcca accagtgagg aagcccagta gttatctggc
     5641 gtgatctgcc gcccgccctc gaagacgtac cctccggcgt ggctgagcgg gtagatgaag
     5701 tgctggacta acgcgtccac gtggctcctc aaacttgaag tacaaatggt tgaggatagc
     5761 gttcataccc ctcttggggc agtgaagtcg attaggcggc cagttggact aggtcactct
     5821 tcgaggagac cgtgggccga gccgaggaag tcaccgacga ccgggccgag cacgctggcg
     5881 ctggtcacca gaccgaggtg accgtcgtga tagacgtaca aggacgagtg cgggatcaac
     5941 gacttcagga gccacgcgtt cgccaacgga atgatggggt cgtcgtcacc ggccaaaatc
     6001 aagaccggct gacgcaacaa cggcagcagc ggcaggctcg tccagcccgc gccagccagc
     6061 atctgatagg cgtagccccg ccgcgacacg cccccacgac tgcgggcgtg agcgagggat
     6121 gccaccaggt cggggtgatt ccgaacctga cccccataga tgctgggcgc cacggccatc
     6181 aaatactccg gatcgcggtg ccgacgcggg gtggccatcg tcagcagcac gttcggccgg
     6241 gccggcacca tgagcgaccc ggtccctgtc gccaccaaca ccacccgacg gcagcggcgc
     6301 gggttctgga acgcgatctg ctgggccaac ccaccgcccc acgagatgcc caagacgtcg
     6361 aaccggtcat acccgagctg gcgcaccaac gctccgatga gcgaggcgag gattgggaag
     6421 cgatacggca gccgagggat cggagagccg ccgacgccag gcgcgtcaaa cccgatgacc
     6481 ggcacgtccg ggtccaacgc ctcgacgaac ggaagcaaca gcgaatagcc ggtgccgatc
     6541 ccgctgcaca acacgagagg cgtccggccc ggcgcaccgg ggcgcgtcca gatccgtagc
     6601 tcgtgaccac ggacggtgac ggtccgcgcg ccggtgacac cggaaagggg catggtcaac
     6661 tccattggct cagggttcga ggacgtaggt gccaggagcc tgcgcgagca cgctcgtgcc
     6721 gccaccatcg agacgcgtgg gcgccttgac gagggcgcca ctgcgcgccg ccaaccaggc
     6781 ggcatagtcc ggccaccacg cgcccgggtg ctgctcggcg ttctcgagcc attcgttcgc
     6841 gtcggtgtag ctggtgctgc cgtcgcggta ggacgccttc gggttgttcg gcgggttgac
     6901 cagagcggcg atgtgcccgc tcgtggacag gatgaatcgc gtgtccccgc cgagcaattg
     6961 ggtgctctgg tagcagttct gccacggcgt gatgtggtcg gcgatgcccg caaccacata
     7021 ggcgtccgtc gtgatctcgc cgaggtccac gggggtcccg agcgtcgtct gcgtgcccgc
     7081 gtgcacgagt cggttctcca tcgcgatatc gatgaagtcg gcatgcaagc cggccggcat
     7141 acgcaccgtg tcggcgttcc agaacaggat gtcgaaggcc ggcgggcggc gcccgagcag
     7201 gtagttgttc acccagtagt tccacacgag gtcaccgggc cgcagccacg cgaacgcctc
     7261 cgcgaggtca cggccatcga tgaagccgcg ctgacgcgac gcgcgcttgg ccaagtcggt
     7321 ggtcctcggt ccggcaagcg ccgcgggcat tcccgcgtgc tcctggtcga gcaccgtcac
     7381 gaagagcgag aacgcggcca gtcggtcaag gcggtcagtg gcagccaggt gcgccacggc
     7441 caacgaggag agtatgccgc ctgagcagat ccccgtcagg gccaccttct cgacaccggc
     7501 cgctttggcg gtgatgtcca tggctttgat gatctcggcc gcgtaggtgt cgctgtccca
     7561 atcagcatga tgcgcgagcg gattgcgcca cgagatgatg aagacctgct ggccctgccc
     7621 gacgaggaac tccacgaggc tgcggccggg ggccaggtcc atggcgtaga acttgttgat
     7681 cgtcggcggc acgataagca gcggcacctc atggaccttg ggcgtcgacg gctgatagcg
     7741 gatcagctcg aagacctcgg tccgggcgat gacggctccg ggcgacaccg cgatgttgcg
     7801 gccgagctcg aagcccgagc tgtcgaccat ctgcggcacg cgcggcttgg cggacatgtc
     7861 cttggcgaag gcggacatgc cacgggcgaa gttggcgccg ccggtgtcga tcgcggcctt
     7921 ggccgacgcg gggttcacca gcgggaggtt gctgggagac agcgcctcgg tgaggttatc
     7981 gaggaggaac tccactcgtt gcgcgtcacg ccatcccagg cccgcgtcgg agaccaactg
     8041 cgccgcggtg gccccggagg cgagatagga ctgcacgagc ctgcgcagca gcgggttctt
     8101 ggcccatgcc tcgtcggcga agcgacggtc gcgccggtct ggagcaaccg tcgacttccc
     8161 agcgacgatc gcagccaggt cggccgcgag ggcccccacc cgccggccgg tggagaccgg
     8221 ccggctggcg agggaaccga gcagacgcgc ggtggagaga tccggggcga agcgacgcag
     8281 cgggcccagc gcggcctcga ccaacaagtg gtcgagcggc acagcgaggt cgtcggtgcc
     8341 ctcagccgga tctacggtga cggtgggcgg catacccaat cagctctttt ccggcgcagt
     8401 gatcatgcgc ttgaggatct tgcccgtggg gcccttgggg agctcgtcga gcagccagac
     8461 cacccgcggg tacttgtagg ccgcgacccg ctcgcgcagg taggtgcgca ggtcatcggc
     8521 ggtcacggtc gacccggcgc ggacgacgac tgctgcaccg acctcttcgc cgtgggtggt
     8581 gtgggcgatg ccgatgacgg cgacctccaa cacgtccggg tgctcgtaga acacctcttc
     8641 gatctcccgc gggtagacgt tgtagccgcc gcggatgatc aggtccttct tgcggtcgac
     8701 gatgaagacg aagccgtcct cgtccttgcg acccatgtca cccgtgagga accacccgtc
     8761 ggggcgcagc gccttcgccg tggcctcggg agcgttccag tagcccttca tgacgttgtc
     8821 cccacggatg gcgatctcgc cgacctcacc gatcgggagt tctttgccgt cctcgtcgaa
     8881 gacggtgatc tccactccgc tgaccggctc gccgaccgag cccaccttgc gcgggcggcc
     8941 catgtggttg aaggaggcag ccggcgaagt ctcagacagg ccgtagccct caagaaccgt
     9001 gcagccccac cgctcctcga agcgagccaa gacctcgacg gggagcgcag caccgccgct
     9061 gacggcgacg cgcaggtgcg gcgcctcgat cgggctgcct tctgccagag ccgaatacat
     9121 cgtcgggacc gcctcgacca ccgtgatgtt gttgtcgagg atgagggcct gcgccgtggc
     9181 cgcgtcgaag cgcgggagca ggacgatcga cgccccacac agcagcgcgg cgttgaggct
     9241 gcaggtctgg ccgaacacgt ggaacagcgg cagaccacca aagacgacgt cgtccttgtc
     9301 catcgagatg aggtcggtcc gcatgagccc ggcgttgctt aggaggttct tgtgggtgag
     9361 ctcggcgccc ttgggaagac cggtcgtccc agaggtgtag aggatgacgg cggtgtcgtc
     9421 cgagccccgc tcgggcacct cggtcaccgg cgtggcagcg gccagccgag gagcgaaggt
     9481 ctcgtcgacg acgatgacgg tgcagccctc cggtgctccg gcgtggacct cgtcggccga
     9541 catggccccc gtgaagatca ggttggcgcc ggagtcgtcg aggtagtgag cgacctcgcg
     9601 gcccttgagc agcgggttca tcggcacgac gatggcgccg gctcgcagga cgccgtagta
     9661 gacgacagcg aactccggga cgttcggcag catgaggccg acgcggtcac cgacaccgac
     9721 tccggcctcg gcgagcaggc cggcggcgcg agcggtgagg ttgtccaact cggcgtaggt
     9781 ggtggtggcg ccggatgcgg tgcgcagcgc gatgcggtcc gcgtgcttgg cggccgaatc
     9841 cacgaaggct tgggcgaggt tcatagcgaa ctcctgtgcg aggaaagggg gcggaagcgg
     9901 ggaagggaga ggaacggaag ggggtggagc gggtgaatcc ggtggagcgg gtgcggtccg
     9961 gtgcggggac gtgtcttaac catccggcat accggctcgt tcggcgaggt gacgcagaac
    10021 cgaactcgct gtgcgcctgc acagtaaggc gccaccacgg gcgggtatgc cgaactacca
    10081 tggcccatgg gcaacgtcgc actgagcctc gccgcgcgca ctctcagcgc gcacctcctg
    10141 gacgagatcg acgaccttgc cgccg
//
LOCUS       DAOMZY010000098        10070 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_6326_length_10070_cov_6.592012, whole genome shotgun sequence.
ACCESSION   DAOMZY010000098 DAOMZY010000000
VERSION     DAOMZY010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 10070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10070
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6326_length_10070_cov_6.592012"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..1079)
                     /locus_tag="PLK64_07175"
     CDS             complement(120..1079)
                     /locus_tag="PLK64_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79562.1"
                     /translation="MALWDELPSALRDSGELDGLRPLLDGLTGTGPVETTEPDGTWST
                     YTATQDLTGPLALDPRTGGFSSSSGSTGTPIEFPDPRVTVELGLHLTGPGGTMDDGWR
                     VILHAPSLRIRMPFLRGAMLDSFGHLRFDAANPIVRFTLPALRVRVQQLSGAAVGVDL
                     LSATTAGPAVDQVYEFIRMEPPYALVGPSDTVGFAFRTAVLDLSGTAGPSGVPAGARA
                     MPSNWQGLFLPDVRLFVAPEGLDGLSVMAGVENLWIGIGVHEGVTGLFEAEVVNRGHA
                     PAIVVRFVTEAGQQIGFTGDSAQLPEHTTVYAESSGGLGAYDV"
     gene            1277..1408
                     /gene="kdpF"
                     /locus_tag="PLK64_07180"
     CDS             1277..1408
                     /gene="kdpF"
                     /locus_tag="PLK64_07180"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HPV79563.1"
                     /translation="MLSAERRIRSDALGGMAEDIVGLVIGLSLVGYLLYALARPERF"
     gene            1415..3061
                     /gene="kdpA"
                     /locus_tag="PLK64_07185"
     CDS             1415..3061
                     /gene="kdpA"
                     /locus_tag="PLK64_07185"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HPV79564.1"
                     /translation="MSPTLQAVLYVTTVALILALAHVPLGEWMYRIFTDERDWRMERL
                     VYRLVGVDPRSEQRSSVYAVSVLAFGVVSVAFLWLLILVQGWLPWGMGRGQNWHTALN
                     TAISFTTNTNWQSYAGEAGAGHTVQMVGLTVQNFVSAATGLAVAAALMRGIARQGADR
                     LGNFWVDLTRACVRLLLPIAAIAAVLLLVGGVIQNLDAPQTITTLAGQQQAVQGGPVA
                     SQEAIKLVGTNGGGFFNANSAHPFENPNGWTNLLQIVLILLIPFALPWTYGRMVGDRR
                     QGAVVTAVMAGLFAVSVTLTTWAERAAAVGVAGSLEGKEMRFGIVPSAIFGAATTSTS
                     TGAVNSMHESYSPLGGGLLMVNMQLGEVSPGGVGTGLYGMLVMVIITVFIAGLMVGRT
                     PELLGKAIGRREITYAALASVTMPALALLFTGASLLIPAARDALLNSGSHGLSEMLYA
                     YTSAANNNGSAFAGLSADQPWLNLSLGFCMFVARFGIIAFTLALAGSLARQKRRPVTE
                     GTMPTHTPLFATLLTGIVLVLAALTFFPALALGPLAEALS"
     gene            3058..5142
                     /gene="kdpB"
                     /locus_tag="PLK64_07190"
     CDS             3058..5142
                     /gene="kdpB"
                     /locus_tag="PLK64_07190"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016663117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HPV79565.1"
                     /translation="MTTTLRPHHHDGHQGPARKPLTAQALENLPQALAKLDPRHLVRQ
                     PVIFVVWIGSVLTTALSVLQPSAFAISITLWLWATIVFANLAEAIAEGRGKAQAATLR
                     QTRTTTTARRLLDDGTEEQISGNELRLGDRVVVEAGQVIPGDGDVIDGVATVDESAIT
                     GESAPVIRESGGDRCAVTGGTTVLSDRIVVQITSKPGETFIDRMIALVEGAARQKTPN
                     EIALGILLTVLTLIFVLAVIALQPMAIYSGGRLSLVVLVALLVCLIPTTIGALLSAIG
                     IAGMDRLVQRNVLAMSGRAVEAAGDVSTLLLDKTGTITFGNRRASDVLPVEGATREEL
                     LRAAYLSSLADETPEGRSIVDYALAEGLTGEVGSAADLLRDGAQFVPFTAQTRMSGVD
                     VATGTRVRKGAGSALARWVHDEGGHMAHDVDDRVDEISTAGGTPLVVAIQEPGTAPRQ
                     LGVIFLKDIVKPGMAERFAQLRQMGIRTVMITGDNPLTAAAIAKEAGVDDFLAEATPE
                     DKMALIKAEQEGGRLVAMTGDGTNDAPALAQADVGVAMNTGTSAAKEAGNMVDLDSDP
                     TKLIDIVGIGKQLLITRGALTTFSIANDIAKYFAIIPAMFVSTFPGLAALNVMRLHSP
                     QSAMLSAVIFNALVIVALIPLSLKGVAYRPLSAAALLRRNLIIYGLGGVLTPFLGIKA
                     IDLLVSLVPGLS"
     gene            5148..5714
                     /gene="kdpC"
                     /locus_tag="PLK64_07195"
     CDS             5148..5714
                     /gene="kdpC"
                     /locus_tag="PLK64_07195"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HPV79566.1"
                     /translation="MTRQLWAATRALLVLTVLLGVAYPLGMTALALAMPDRATGSLVM
                     IDGRPAGSVLLGQATVEPQWFRARPSVSDYSGETSGGSNWGPSAAELSADLAARAADL
                     RAVNPLAPAGPIPADALTTSASGLDPHISPAYAAWQAPRVAAARGMTVEQVQRLIDAN
                     MTSGELGFLGADRVNVTTLNAALATLGR"
     gene            5738..8239
                     /locus_tag="PLK64_07200"
     CDS             5738..8239
                     /locus_tag="PLK64_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPV79567.1"
                     /translation="MARGRLRIYLGAAPGVGKTVAMLSEGARRKARGTDVVVGVVETH
                     DRAHTAAMLDGLEIVPRRTVPHRGATITEMDLPAVLARHPQVVLVDELAHTNAPGSEH
                     HKRWQDIEALLAAGIDVISTVNIQHLESLNDAVEDITGIVQQERVPDAVVRAADQIEL
                     VDMSPEALRRRMAHGNVYKSDKVDAALSHYFRVGNLAALRELALLWLADRVDEGLDLY
                     RHAHDIDATWPTRERIVVAVSGGPESEALVRRGARIAARGAGGELVVLYVARADGLAG
                     APVEALAKVRRMVEEVGGTFHTISGEDRARAILQFARAENASTILVGASRRPWWRALV
                     RPGVAAPVIEGSGDIDVHVVTHSAAGTRHAYVARAPLARRRVVEGYAWGVVGGAVLTA
                     VLWQLDARLGQPLGLPLVVQLYLVHTIVASLIGGMAPAIVTAVISSLTVNWFFTPPIG
                     NLTIAAPENALALALFIVVAAAVSLVVHRSSRRAETALAAQQESAALAELTHTLLGST
                     EQLALLLSRAIDMFGVTGAAVVRRRTKDEPDSVIAATDGFDLSGGATTTEAVDEEHDL
                     ILTGGPVPAERKRLLAAYSAHAGAILARRALQDQARSAEHLTRDNKARTALLSAVSHD
                     LRTPLAGIKAAIGSLRSHEVEFSPEDRAELEEAIDDSADRLESLIGNLLDMSRLQVGA
                     LVAHPRLTDLGEIVPAAIIGSAATGRVTWTLGEGARYAEADPGLLDRVLSNLVENAMR
                     HHDDPAKIRVTTSRIRDEVQLRVIDSGPGIPEAMREPAFQPFQRHTDSGGSGVGLGLA
                     VARGLAEAMGGTLTAEETPGGGLTMVVALPAGVSP"
     gene            8236..8928
                     /locus_tag="PLK64_07205"
     CDS             8236..8928
                     /locus_tag="PLK64_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPV79568.1"
                     /translation="MSARVLVVDDDPTILRTLRINLRARNYEVEAVTNGRDALSAFME
                     APPDLVVLDLGLPDVDGVEVLRRMRRISRVPVVVLSARQDSDDKVEALDEGADDYVTK
                     PFGMDELMARIRAAIRRGGEALASTPPFSCDDFTLDFDERTCEASGSIVHLTPTEWRL
                     LAELARHDGRIVPHENLLKAAWGPTYGRESNYLRVYVSQLRRKLEPDSSRPRHLLTEP
                     GLGYRLVRRGHD"
     gene            complement(8962..9834)
                     /locus_tag="PLK64_07210"
     CDS             complement(8962..9834)
                     /locus_tag="PLK64_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HPV79569.1"
                     /translation="MRALEVVATGPLALVEDLGRLGYAAVGVGRSGAADRAAYLLAGR
                     LLGEPVGRAAVEVTLGGLVVRARGSLTAVVTGAAAPATVDGRPVGHAAPVHLPDGAVL
                     ALGMPPTGLRSYLAVRGGIDLDPVLGSRSSDTLSGIGPAPLRVGQVVPVGEPGAGGPT
                     MDVAPVAAPEGGRVELTLLPGPRLDWLADPRALTATPWTVSGSSDRVGMRLTGPALSR
                     AERWADAELPSEGVVVGGVQVPADGQPIVFLADHPVTGGYPVVAVVRAADVDRAAQCR
                     PGQDVRLRWAVAPR"
     gene            complement(9834..>10070)
                     /locus_tag="PLK64_07215"
     CDS             complement(9834..>10070)
                     /locus_tag="PLK64_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HPV79570.1"
                     /translation="VGGDPRLVVPRRAEPRPRVAAGSVALAGPYSGIYPTDSPGGWQL
                     IGRTEVTLWDLHRDPPAVLRPGMRVRFRNVGGPP"
BASE COUNT         1507 a         3565 c         3434 g         1564 t
ORIGIN      
        1 gtcgtgacga cgggtccacc gccgaccgcg gtgagggcga cgacgaccga gatcgacccg
       61 gcgggtggcg tggtgacggg cgcgcggtcg tcgtcggtgc ggacgccgtt gacggtgatc
      121 tacacgtcgt aggcgccgag cccgccggac gactcggcat acaccgtggt gtgctcgggg
      181 agttgcgcgg agtctccggt gaagccgatc tgttggcccg cttcggtgac gaatcgcacg
      241 acgatcgcgg gggcgtggcc gcggttgacg acctcggcct cgaagagccc ggtgacgcct
      301 tcatggacgc caatgccgat ccagaggttc tccacgccgg ccatgaccga gaggccgtcg
      361 agcccttcgg gcgcgacgaa caggcggaca tcgggcagga agaggccctg ccagttgctc
      421 ggcatcgccc gggcgccggc gggcacgccg ctcgggcccg cggtcccgga caggtcgagg
      481 accgccgtgc ggaaggcgaa cccgacggtg tcggacgggc cgacgagggc gtagggcggt
      541 tccatccgga tgaattcata gacctggtcg accgcagggc cggccgtggt cgccgagagc
      601 aggtcgacgc cgacggctgc gccggagagt tgctgcaccc gaacccgcaa cgcgggcagg
      661 gtgaaccgga cgatcgggtt ggccgcgtcg aagcgcaggt gcccgaagga atcgagcatc
      721 gcgccgcgca ggaacggcat ccggatccgc agggacgggg cgtgcaggat gacccgccag
      781 ccgtcgtcca tggtgccgcc gggaccggtg aggtgcaggc ccaactcgac cgtcacccgg
      841 gggtccggga actcgatcgg ggtgcccgtc gagccgctgg agctgctgaa gccgcccgtc
      901 cgcgggtcga gggccaacgg cccggtgagg tcctgggtcg cggtataggt cgaccacgtc
      961 ccgtcgggtt cggtggtctc gaccgggccg gtgccggtca gcccgtcgag cagcggacgt
     1021 agaccgtcaa gctcgcctga gtctcggagc gcagaaggca gctcgtccca caacgccatg
     1081 gcacttcctt gggagagttg gaagttcgag cgtggacaac gccggggcgt cgtgggtcct
     1141 agtggagcac agaccagtgc gaggcgcgcg ccgaatgccc ggattgcctc tcgatttcct
     1201 cttgcagccc tctcggccgg gtcgacccgt tgccagtggg gccgtcaaga tcgtgtcaag
     1261 aagcgctagg agctccttgc tgtcggcgga gcgccggatc cggtcggatg cacttggggg
     1321 catggcagaa gacatcgtcg ggctcgtcat cggcttgagc ctcgtcggct atttgctcta
     1381 cgcgctcgcg cgccccgaga ggttctgagt cgaggtgagc cccaccctgc aggccgtcct
     1441 ctatgtgacg acggtggccc tcatcctcgc tctggcgcac gtgccgctcg gcgagtggat
     1501 gtatcggatc ttcaccgatg aacgcgactg gaggatggaa cgcctcgtct accgactcgt
     1561 cggtgtcgat ccgcgctccg aacagcgctc gtcggtctac gcggtgagcg tgctggcctt
     1621 cggcgtggtg tcggtggcct tcctctggct gctcatcctc gtccagggct ggctcccgtg
     1681 gggcatgggc cgcggccaga actggcacac cgccctcaac acggcgatct cgttcacgac
     1741 caacaccaac tggcagtcgt atgccggtga ggctggtgcc ggccacaccg tccagatggt
     1801 gggtctcacc gtccagaact tcgtgtcggc cgccaccggc ctcgccgtcg ccgcggcgct
     1861 catgcgcggc attgccaggc agggcgccga ccgactcggc aacttctggg tcgacctgac
     1921 tcgggcctgc gtgcggctgc tcctgccgat cgcggcgatc gccgccgtgc tcttgctcgt
     1981 cggtggcgtc atccagaatc ttgatgcccc gcagacgatc acgaccctcg ccggtcagca
     2041 gcaggccgtc cagggcggcc ccgtcgccag ccaagaggcg atcaagctcg ttggcaccaa
     2101 cggcggcggc ttcttcaacg ccaactcggc gcaccccttc gagaatccga acgggtggac
     2161 caatctcctc cagatcgtcc tcatcctgtt gatccccttc gccctgccat ggacctacgg
     2221 ccggatggtc ggcgaccgtc gccagggcgc tgtcgtcaca gcggtgatgg ctggattgtt
     2281 cgcggtctcg gtcaccctca cgacgtgggc cgagagggcc gccgcagtcg gcgtcgcggg
     2341 ctcgttggag ggcaaggaga tgcgattcgg gatcgtgccc tcggccatct ttggggcggc
     2401 gacgaccagc acctcgacgg gtgcggtcaa ttcgatgcac gagtcgtaca gcccgctcgg
     2461 gggtggcctg ctcatggtca acatgcagtt gggcgaggtg tcccccggtg gggtcggcac
     2521 ggggctctac gggatgctcg tcatggtcat catcaccgtg ttcatcgctg gcctgatggt
     2581 cggccgcacg cccgaactcc tcggcaaggc gatcggccgc cgcgagatca cgtatgccgc
     2641 gctcgcctcg gtgacgatgc cggccctcgc cctgctcttc accggtgcgt cgctgctgat
     2701 cccggccgcc cgagacgccc tgctcaacag cgggtcgcac gggctgtcgg agatgctcta
     2761 cgcctacacc tcggcggcca acaacaacgg ctcggcgttc gcaggcctct cggccgacca
     2821 gccctggctc aacctctcac tggggttctg catgttcgtc gcgcggttcg ggatcatcgc
     2881 cttcaccctg gcgctggcag gcagcctcgc ccggcagaag cgccgccccg tgacggaggg
     2941 cacgatgccc acccacactc ccttgttcgc gaccctgctg accggcatcg tcctcgtcct
     3001 cgccgcgttg accttcttcc cggccctggc gcttggccct ctggcggagg ctctctcatg
     3061 acgaccacgc ttcgaccgca ccaccacgac ggacaccagg ggccggcccg caagccgctc
     3121 actgcccagg ccttggagaa cctgccccag gcactggcca aactcgaccc tcgacacctg
     3181 gtccgtcagc cggtgatctt tgtcgtttgg atcggctcgg ttctgacgac ggcgctgtcc
     3241 gtgctgcagc ccagcgcctt cgcgatctcc atcaccctgt ggctgtgggc gacgatcgtc
     3301 ttcgccaacc tcgcggaggc tatcgctgag ggccgaggca aagcgcaagc ggcgactctc
     3361 cgccagaccc gcaccacgac cacggcccgg cgactgctcg atgacgggac ggaggagcag
     3421 atctctggca acgagcttcg gctgggcgac cgagtcgtcg tcgaggcggg ccaggtgatc
     3481 cctggagacg gcgacgtcat cgacggcgtc gcgaccgtcg acgagtcggc catcaccggc
     3541 gagtccgccc ccgtgatccg cgaatccggc ggcgaccgct gcgcggtcac gggcggcacg
     3601 acggtgctgt cggaccggat cgtcgtccag atcacgagca agccgggcga gaccttcatc
     3661 gaccggatga tcgcgctggt ggaaggcgca gcgaggcaga agacgccgaa cgagatcgcc
     3721 ctcgggatcc tgttgaccgt cctcacgttg atcttcgtcc tggcggtcat cgccctccaa
     3781 ccgatggcca tttactccgg cgggcgcctg tcgctcgttg tgctggtcgc cctcctcgtc
     3841 tgcctcatcc ccaccaccat cggtgcgctg ctgtcggcca tcggcatcgc cgggatggac
     3901 cggctggtcc aacgcaacgt gttggccatg tccggccgcg ccgtcgaggc ggccggtgac
     3961 gtgtcgaccc tgctgctgga caagacgggg acgatcacct tcggcaatcg ccgggcctct
     4021 gacgtccttc cggtcgaagg ggcgacgcgt gaggaactgt tgcgcgcggc atacctctcc
     4081 tcgctggccg acgagacgcc tgagggacgg tcgatcgtcg attacgcgct cgcggagggc
     4141 ctcacgggcg aagtgggttc cgccgccgat ctcctgcggg acggcgccca gttcgttccg
     4201 ttcacggcgc agacccgcat gtccggggtc gacgtcgcta cgggaacgcg cgtgcgcaag
     4261 ggcgccggga gcgcgttggc ccggtgggtg catgacgagg gtggccacat ggcacacgat
     4321 gtcgatgacc gggtcgacga gatcagcact gcgggtggaa ccccgctggt cgttgccatc
     4381 caggagccgg ggactgctcc ccggcaactc ggcgtgatct tcctcaagga catcgtcaag
     4441 ccaggcatgg ccgagcggtt cgcgcagttg cgtcagatgg gcatccgcac ggtgatgatc
     4501 accggcgaca accccctcac cgccgcggcg atcgccaagg aggccggggt agacgacttt
     4561 ctcgccgagg ccacgcccga ggacaagatg gccctcatca aggctgagca ggagggcggc
     4621 cgtctcgtcg cgatgaccgg cgacggcacg aatgacgctc cggcgcttgc gcaggccgac
     4681 gtcggcgtgg cgatgaacac cggaacctcg gcggccaagg aagccggaaa catggtggac
     4741 ctcgattcgg atccgaccaa gctcatcgac atcgtcggta tcggcaagca gttgctgatc
     4801 acccgcggag cgttgaccac cttctcgatc gccaatgaca tcgctaagta cttcgcgatc
     4861 atccccgcca tgttcgtcag caccttcccc gggctcgcgg cgctcaacgt catgcggctg
     4921 cactccccgc agtcagcgat gctgtccgcg gtcatcttca acgcgttggt catcgtcgcc
     4981 ctgatcccct tgtcgctcaa gggtgtggcc tatcgaccgc tcagcgcggc ggccctgctg
     5041 cgacgcaacc tgatcatcta cggccttggc ggcgtcctga cgccgttcct tggcatcaag
     5101 gccatcgacc tgctcgtttc cctcgtcccc ggattgtcgt gacagccatg accagacagt
     5161 tgtgggctgc gacccgcgcc ctcctcgtcc tcaccgtcct cctcggtgtc gcctacccgc
     5221 tgggcatgac cgccctcgcc ttggcgatgc ctgatcgggc taccggttcg ctcgtcatga
     5281 ttgacggtcg gcccgcgggt tcggtgctgc tgggtcaggc cacggtcgaa ccgcagtggt
     5341 tccgggcccg accgtcggtg tcggactact cgggggagac cagcggcggc agcaactggg
     5401 gcccgtccgc tgccgagttg tcagccgatc ttgcggcgcg cgcagccgac ctccgcgcgg
     5461 tcaacccgtt ggcgcccgcc ggcccgatcc ccgctgacgc cctcaccacc agcgccagcg
     5521 gcttggaccc gcatatctcc ccggcgtatg ccgcatggca ggcgcccagg gtcgccgccg
     5581 cccgcgggat gaccgttgag caggtgcaac gactgatcga cgcgaacatg acatccgggg
     5641 agctcggctt cctcggcgct gatcgcgtca acgtcaccac cctcaacgcg gccctcgcca
     5701 ccctcgggcg ctgacggcat acgggacaat cgacaccatg gcacgcggcc gactgcggat
     5761 ctacctcggg gctgcccccg gggtcggtaa gacggtcgcc atgctttcgg agggtgcccg
     5821 acgcaaggct cgtggcacgg atgtcgttgt cggggtcgtc gagactcacg atcgcgctca
     5881 caccgcggcg atgctcgacg gccttgagat cgtgccgcgc cgcacggttc cgcaccgcgg
     5941 ggcgacgatc accgagatgg acctccctgc ggtgctggcc cgtcacccgc aggtggtgct
     6001 ggtcgacgaa ctcgcccaca ccaacgctcc cggatctgag caccacaagc gctggcagga
     6061 catcgaggcg ttgctggcgg ccggcattga cgtgatctcg acggtgaaca tccagcacct
     6121 ggagtcgctc aacgacgccg tcgaggacat caccgggatc gtccagcagg agcgggtgcc
     6181 cgacgccgtc gtccgagccg ccgaccagat cgaactcgtc gacatgtcgc ccgaagcact
     6241 gcgccgtcgg atggcccacg gcaacgtcta caaatctgac aaggtcgacg ccgccctgtc
     6301 gcactacttc cgggtcggca acctcgccgc cttgcgggag ctcgcgctcc tctggcttgc
     6361 cgaccgcgtc gacgaggggc tggacctcta tcgacatgcc cacgacatcg acgccacctg
     6421 gcccacccgc gaacggatcg tcgtcgcggt ctccggcggc ccggaatccg aggccctcgt
     6481 cagacgggga gcccggattg cagcgcgcgg agcaggtggt gagctcgtcg tcctttacgt
     6541 cgcgcgagcc gacggtctgg ctggcgcgcc cgtcgaggca ctcgcgaagg tgcggcgcat
     6601 ggtcgaggag gtcggcggca cgttccacac gatctccggc gaagaccgcg cgcgcgccat
     6661 cctccagttc gctcgcgccg agaacgcctc gacgatcctc gtcggtgcca gccgccggcc
     6721 atggtggcgg gcgctcgtcc gacctggcgt tgcggccccg gtcatcgagg gatctggtga
     6781 catcgacgtc cacgtcgtca cgcatagcgc tgccggcacc cggcacgcat acgtcgcgcg
     6841 ggcgccgctg gcccgccgac gcgtggtcga gggctatgcc tggggggtgg tcggcggggc
     6901 cgtcctcacg gccgtcctgt ggcagctcga tgctcgactc ggccagcccc tggggctgcc
     6961 cctcgtcgtc cagctctacc tcgtgcacac catcgttgcg tcgctgatcg gtgggatggc
     7021 cccggcgatc gtcaccgccg tcatctcctc actcaccgtg aactggttct tcaccccgcc
     7081 gatcgggaac ttgacgatcg cggcgcccga gaacgccctg gccttggccc tgttcatcgt
     7141 tgtggcggca gccgtatcgc tggtcgtgca ccgcagttcc cgccgggcgg agacggcgtt
     7201 ggccgcgcag caggagtctg ccgccctcgc ggagttgacc cacacgttgt tgggatcgac
     7261 cgagcagctc gcgctcctgc tgagccgcgc gatcgacatg ttcggtgtca ccggggcagc
     7321 cgtcgtccgc cgccggacga aggacgagcc ggactcggtc atcgcggcca ccgatgggtt
     7381 cgacctcagc ggcggcgcca cgaccaccga ggccgtggac gaggagcacg acctcatcct
     7441 caccggaggg ccggtgcctg ccgagcgcaa gcggctgctc gcggcatact ccgcccacgc
     7501 cggagccatc ctcgcgcgcc gcgccctaca ggatcaggcg cgcagcgcgg agcacctgac
     7561 ccgggacaac aaggcccgga ccgcgctgct gtccgcggtg tcccacgacc tgcgcacccc
     7621 gttggcaggg atcaaggcgg ctatcggcag cctgcgcagt catgaagtcg agttcagccc
     7681 cgaggaccga gccgagctcg aggaggcgat cgacgactcg gccgaccggc tggagtctct
     7741 gatcgggaac ctcctggata tgtcccgcct ccaggtcggc gcgctcgtgg cgcacccgcg
     7801 gcttaccgac ctcggcgaga tcgtgccggc cgcgatcatc gggtcggccg ccaccggacg
     7861 ggtcacctgg accctgggcg agggagcgag gtatgccgag gccgaccccg gcctcctgga
     7921 ccgggtgctc agcaaccttg tcgagaacgc catgcgccac catgacgacc cggccaagat
     7981 ccgggtgaca accagtcgga tccgggacga ggtccaactt cgagtgatcg actcgggccc
     8041 agggattccc gaggcgatgc gcgagccggc cttccaacct ttccaacggc ataccgactc
     8101 tggggggagc ggcgtcggtc tcgggctggc cgtcgcgcgc gggctggccg aagcgatggg
     8161 cgggacgctc accgcggagg aaactcccgg cggtggcctc accatggtcg tcgccctccc
     8221 cgccggggtg tccccgtgag cgcccgagtg ctcgtcgtcg acgacgatcc gaccatcctg
     8281 cggacgctga ggatcaacct tcgcgcgcgc aactacgagg tcgaagcggt caccaacggc
     8341 cgcgacgcct tgagcgcctt catggaggcc ccacccgacc tcgtcgtgct ggacctgggg
     8401 ttgccggacg ttgatggcgt cgaggtgctg cgacgaatgc ggcggatcag ccgggtgccg
     8461 gttgtcgtcc tgtccgcccg gcaggacagc gatgacaagg tggaagccct ggacgagggt
     8521 gccgacgact acgtgaccaa gcccttcggg atggatgagc tgatggcccg gatccgcgcc
     8581 gcgatccgcc gcggcggtga ggcgctggcc tccactccac cgttctcgtg cgatgacttc
     8641 accctcgact tcgacgaacg gacctgcgag gcaagcggat ccatcgtgca cctcaccccg
     8701 accgagtggc gcctgctggc cgagctggcc cgccacgacg gacgcatcgt cccccacgag
     8761 aacctcctca aggctgcctg gggacccacc tacggacggg aatccaacta cctacgcgtc
     8821 tacgtgagcc aactccggcg caagttggag cccgactcat cccggccccg ccacctgctg
     8881 accgagcccg gtctcggtta ccgcctcgtg cgacgaggcc acgactgacg gcggttgcgt
     8941 cggtggggca ggctgcggga ttcagcgggg ggcgactgcc caacgcagcc gcacgtcctg
     9001 gccggggcgg cactgggctg ctcgatccac atcggcggcc cggacgaccg cgacgacggg
     9061 gtagccgccg gtgaccgggt ggtcggcgag gaacacgatc ggctgtccgt cggcgggcac
     9121 ctggacgccg cccacgacga ctccctcgct gggcagttcg gcgtccgccc agcgctcggc
     9181 ccggctcagc gccggcccgg tcaggcgcat tccgacccga tcactgctcc ccgacaccgt
     9241 ccatggcgtg gctgtcagag cccgtggatc ggcgagccag tccaggcgag gtccaggcag
     9301 cagggtgagc tcgaccctcc ctccctcggg tgccgcgaca ggggcgacgt ccatggtggg
     9361 tcctcccgcc ccgggctcgc cgacgggcac cacctgaccc acccgaagcg gcgccgggcc
     9421 gatccccgac agggtgtcgc tggaccgcga cccgaggaca gggtccagat cgatcccccc
     9481 gcggacggcg aggtagctgc gcagcccggt gggcggcata cccaacgcga ggacggcccc
     9541 gtcggggagg tgaacgggcg ccgcgtgacc gaccggtcgg ccgtcgaccg tggcaggtgc
     9601 ggccgcgccg gtgacgacgg cggtgagcga gccgcgggcc cgcacgacga ggccgccgag
     9661 ggtgacttcc acggcggcgc gcccgaccgg ctccccgagc agacgccccg cgaggaggta
     9721 tgccgcccgg tcggctgccc cggaacgccc tactcccaca gcggcatacc cgagtcgccc
     9781 gaggtcttcg accagggcga gcggcccggt ggcaaccacc tcaagcgcgc ggatcacggc
     9841 gggccgccca cgttgcgaaa gcgcacccgc atccccggcc gaagcaccgc gggagggtct
     9901 cgatgcagat cccagagggt cacctcggtg cgcccgatga gctgccaacc gccgggtgag
     9961 tcggtggggt agatcccgct gtaggggccg gcgagggcga ccgaaccggc ggcgacgcgc
    10021 gggcgaggct cggcccggcg cgggacgacg agcctcgggt cgccgccgac
//
LOCUS       DAOMZY010000099         9919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_6455_length_9919_cov_6.074615, whole genome shotgun sequence.
ACCESSION   DAOMZY010000099 DAOMZY010000000
VERSION     DAOMZY010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9919
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6455_length_9919_cov_6.074615"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2874
                     /gene="hrpA"
                     /locus_tag="PLK64_07220"
     CDS             <1..2874
                     /gene="hrpA"
                     /locus_tag="PLK64_07220"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HPV79571.1"
                     /translation="SLTVPGIRYVVVTGTARISRYSQRTKVQRLPIEPVSKASARQRS
                     GRCGRLADGICIRLYSEEDFEARPDFTDPEILRTNLASVILQMTTLGLGEVAAFPFVD
                     PPDDRNITDGVRLLEELRAFEQGEGADSGRRLTAYGKTIALLPVDPRLARMVVEADRL
                     GCLLDVLVVVAVLSIQDPRERPLEQQEAAALSHKRFADEHSDFVAYLRLWDYLRERQE
                     ELSGSAFRRMCKSEFLHYLRIREWQDLVGQLRQACKSAGFAASARSQGERDVDAIHKS
                     LLSGLLSQVGVRDEAKRDYLGARSARFSISPGSGLFRKQPQVVMAGELVETTRLWAHD
                     VARIDPVWAEELGSHLVKRQYSEPRWSSRQGSAVATERVTLYGVPLVVGRTVGLAKVD
                     PALARELFIRHALVEGDWTTHHRFFHDNRALLERLSELEARTRRRDILVGDDVLQDFY
                     EHRLPAEVVSARHFDSWWKRARRTTPDLLTFTEDLLVRDEAGDIGADAFPTTWHQGDL
                     DLPLTYQFEPGAAADGVTVHVGTEILNRVASDGFDWQVPGLREELVTALIRTLPKTTR
                     RLLVPAPEHAAATVRELRQRALGPADGPLTVVLAQVLRETHGVVIRPDDWDIARLPDH
                     LRVTFAVEDRPGHAIAAGKDLAALREQAAPQVRRQVAAAGRAITRTGMRTWAIDDVPL
                     EHDAVVSGVRVHGFPALVDEGDAVALRVLPTPAEAESEHRLGVRRLLLLGTTPPWKHV
                     LGRLTNAQKLALGHNPHGSVPALLDDVLAAAVDAIAAERVHHVPRTAAEFAAAQDAVR
                     THLGARVVAAVGLVEPILAKHLQIVRRLDGMSAPHLADLRADVRAQLAELIRPGFVAD
                     TGLARLPDLDRYLRAVLHRLDKAPLDLARDSQRLTEVLDAEAGYAALLASLRPSQRGA
                     AQVTDIAWMIEELRVSLFAQSLGTAYPISVKRIEKAIGAIPRD"
     gene            complement(2893..3324)
                     /locus_tag="PLK64_07225"
     CDS             complement(2893..3324)
                     /locus_tag="PLK64_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79572.1"
                     /translation="MTTPQDALPAPASGEIAVTISLPWTIGVSIKPNVLLDGVRMVNA
                     AGRNVYPVAPGRHMLSCEVNVSGSTFGQGDIAVDVQPGVTTEVYYAAPLSENLPGRLG
                     LTPQTPYRGGAAGCILAAVIGFLVLFVFWVVIGGFLAVGRR"
     gene            3458..4378
                     /locus_tag="PLK64_07230"
     CDS             3458..4378
                     /locus_tag="PLK64_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HPV79573.1"
                     /translation="MQDPTALYRFENDADPAEVRAGVLVAALGGFVDAGGTQRLLTAH
                     LLENHSSQVVASFDVDQLLDYRGRRPTMTFDKDRWTAYDDPSLVLHRVVDRAGTPFLL
                     LAGPEPDYQWERVTAAILQLVAALGVQLTVSVHGIPMAVPHTRPLGTTPHATTGRLLG
                     EVEPVFGTIQVPGSIAGLLELRLGEAGRDAMGYAVHVPHYLAQADFPDASLVAFDALV
                     GATGLDLARDELATSALHTREQVATELADNDEVRTLVVSLERQYDAFMEGRTRPSLLA
                     TGMGEVPSADEIAADVEAFLREVNDEGRQA"
     gene            complement(4460..6169)
                     /locus_tag="PLK64_07235"
     CDS             complement(4460..6169)
                     /locus_tag="PLK64_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HPV79574.1"
                     /translation="MVAPGNELVPLVVVGERAAYLHSGMPRVGSARDVLADAARLEAD
                     EHPRWAVPSAATSLRALVDAGVGPARSWDLAEGHRILHGGWAAGPGLVHAVLRGLAPE
                     GIPQAREADLFDLAVDPLADEHPDAPIGPDGYLRADAFTPAWADRDARLLALAAFVGD
                     LAGSQRERLGSVGARAVATAYSESAAAVLCLELERDGLPIDRPRAEALVAAAAGARPT
                     SEADAARIRGERDALVLRHAPGRRADLRNPAQVKDLLAAAGVEVATTRKWVLEPYRAT
                     HPIVAALLEWRKDERIATTYGYRWLDEQVGHDDRLRGAWTASDGAAGRMTASAGLHNL
                     PVPLRPAVAAEPGFVFVRADLGQIEPRVLAAVSGDAAFAEATLADDLYAPVAAKLRVE
                     RSVAKIAVLAAMYGQRSGAAGEALRDLQRAYPVAMGLLDEAYAAGLARRPLRTFGGRL
                     IRLDSALAPEAGQVVGEGWDAARGRFARNAIIQGAAAELFKAWAATVRHHLPSLGGQI
                     VLCLHDELLLHVPVEASEAVAALVHQTLTDSSRRWVGASAGPPVRFVADVSIINRWDE
                     AKG"
     gene            6345..7073
                     /locus_tag="PLK64_07240"
     CDS             6345..7073
                     /locus_tag="PLK64_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HPV79575.1"
                     /translation="MGTFGKGESSELSSVPRGALERAADSESEGGLTKVATELVEKLL
                     EVGIDGRGPVKSAHEVAVAARAEVRSDDAAIKVIIRKHVRAAAVGGFITGLGGFVTMP
                     VALPANVAEFYLIATRMVAAIADVRGYDVTDPQIRTAVLLTLVGVDANEVVKKAGAIP
                     GVSFGPGGTLTALASQRLPGPALMVLNKAIGFRLVAQAGRTLLGRLGKAVPVVGGAIG
                     AGVDGYLLSRIADGATREFPRRAD"
     gene            7178..7639
                     /locus_tag="PLK64_07245"
     CDS             7178..7639
                     /locus_tag="PLK64_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HPV79576.1"
                     /translation="MAAARGASSARWGMIRALGLALRAATRPGAPGMGERLSALPRMV
                     RAAMRGEYAGVTRGRLLGMLGALLYVVSPVDLVPESLFTIFGLADDALLVTWLASSLV
                     NDTEAFLTWERGLSRAGSTSAAGGPQDAPAGERTTPEDSSAHWTVQSHVVR"
     gene            7757..8959
                     /locus_tag="PLK64_07250"
     CDS             7757..8959
                     /locus_tag="PLK64_07250"
                     /inference="COORDINATES: protein motif:HMM:NF025200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4192 domain-containing protein"
                     /protein_id="HPV79577.1"
                     /translation="MTTTIRITDPVDLVKVVPYQLGYHPERSLVLIGLRRKQIGLVQR
                     LDLPTLPRDCEAAADLMAGHLRKDGCTGAVLVLYEDTEGEGAVAAAALVARLQVGRFD
                     LVEHVVVRDGHVYFGALAGRADRPVSGVLLPPDDQVPAVADYVAHGRRPAPSRGSLDE
                     RLAPEDDALSERVRLAAARLGVLRPVLGSRPKAMADWGAFLDVGDDAWFAGTLPSAAA
                     TARMCHSLRDLHLRDLVTAWLCPGTLELSAFDEDLRALAVEHLPAGRAWPEAYAAAGH
                     SPAAGGVADDAPAARPRGGAGSRRRRAASSKPRRVVTAVAGPRTDEDDRLVERLCWLA
                     RHTPEVHASAVLSVLASVTWHLGDGTLTRVALDRALALDPGYRLALLIERMVDLAIRP
                     GRALSSTG"
     regulatory      9005..9116
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00634"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class IV; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00634"
     gene            9132..>9919
                     /locus_tag="PLK64_07255"
     CDS             9132..>9919
                     /locus_tag="PLK64_07255"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HPV79578.1"
                     /translation="MTVSDDPPFVTGAWRDGDPVGHRAFVDFPELHLERGGVLPSVRV
                     AYETWGTLNPAGSNAILVEHALTGDSHVVGAAGPGHASPGWWEGLVGPGRDIDTDEWF
                     VVAANVIGGCQGSTGPASPAPDGRPWGSRFPFVTIRDQVAAEAALADALGIEAWALVI
                     GGSMGGMRVIEWAVTYPDRVAQAVILASTAAATADQIAWAQPQLLAIRSDPDFAGGDY
                     YGHSRGPDVGLGIARRIAQATYRSASELDERFGREPQSEEDPLGG"
BASE COUNT         1399 a         3563 c         3530 g         1427 t
ORIGIN      
        1 tcgttgaccg tccccggcat ccgctacgtc gtcgtcacgg gcacggcccg gatctcgcgc
       61 tacagccagc ggaccaaagt gcagcggctg ccgatcgagc cggtctccaa ggccagcgcc
      121 cgccaacgct ccgggcggtg cgggcgcctg gccgacggga tctgcatccg gctctactcc
      181 gaagaggact tcgaggctcg gccggacttc accgaccccg agatcctgcg gaccaacctc
      241 gcctcggtca tcctccaaat gaccaccctc gggctgggcg aggtggccgc gttcccgttc
      301 gtcgacccgc ccgacgatcg gaacatcacc gacggggtgc ggctgctgga ggagttgcga
      361 gccttcgaac agggggaggg ggccgactcg gggaggaggc tcacggcata cgggaagacc
      421 attgccctcc tgcccgtgga cccgcgcctg gcgcggatgg tcgtcgaagc cgaccggctt
      481 gggtgcctcc tcgacgtgct cgtcgtcgtc gcggtcctgt cgatccagga cccccgcgag
      541 cgcccgctgg agcagcagga ggcagcggcc ctgtcccaca aacgttttgc cgacgagcac
      601 tccgacttcg tcgcttacct gcggctgtgg gactacctga gggagcggca ggaggagttg
      661 tcagggagcg cgttccggcg gatgtgcaag agcgagttcc tgcactacct gcggatccgc
      721 gagtggcagg atctcgtcgg acaactgcga caggcctgca agagtgcggg attcgcggcc
      781 agcgcgcgct ctcagggcga gcgtgatgtc gacgcgatcc acaagtcgct gctgtccggg
      841 ctgctgagcc aggtcggcgt gcgcgacgag gccaagcgcg actacctcgg cgcgaggagt
      901 gcgcggttca gcatctcgcc gggctcgggg ctgttccgca agcagccgca ggttgtcatg
      961 gccggcgaac tcgtcgagac gacccggctc tgggcccacg acgtcgcgcg gatcgacccg
     1021 gtgtgggccg aggagctcgg cagccatctg gtcaaacggc agtactccga gccccgctgg
     1081 tcctcgcggc agggctcggc tgtcgccacc gaacgggtca ctctctatgg cgtcccgctt
     1141 gtcgtcggac ggaccgtcgg gctggccaag gttgacccgg cgctggctcg ggagctgttc
     1201 atccgccatg ccctcgtcga gggtgactgg acgacccacc accggttctt ccacgacaac
     1261 cgcgcactgc tcgagcgact ctcggagctc gaggctcgca cccggcgtcg cgacatcctc
     1321 gtcggcgacg acgtcctgca ggacttctac gagcatcggc tccctgcgga ggtcgtctcg
     1381 gcccgccact tcgactcgtg gtggaagcgg gcccggcgca ccactcccga cctgctcacc
     1441 ttcaccgagg acctgctggt gcgcgacgag gcaggcgaca tcggcgccga cgccttcccg
     1501 acgacctggc accagggcga cctcgacctg ccgttgacct accagttcga gcccggcgcg
     1561 gcggctgacg gagtcaccgt ccacgtcggc accgagatcc tcaatcgggt cgcctcggac
     1621 ggattcgact ggcaggtgcc gggattgcgc gaggagttgg tcaccgcact catccgcacg
     1681 ctgcccaaga cgactcgtcg gttgctcgtg ccggctccgg agcacgccgc cgcgacagtc
     1741 cgggagttgc gccagcgcgc gctcgggccg gccgacggtc cgctcaccgt cgtcctggcc
     1801 caggtgctgc gtgagaccca cggcgtcgtc atccgccccg acgactggga catcgcccgg
     1861 ctgcctgacc acctgcgagt gacgttcgcg gtggaggacc gccccgggca cgccatcgcc
     1921 gcgggcaagg acctggccgc tctgcgcgaa caggccgccc ctcaggtgcg ccggcaggtg
     1981 gcggccgccg gtcgcgccat cacgcgaacg ggcatgcgca cgtgggcgat cgacgacgtg
     2041 ccgctcgaac acgatgccgt cgtgtcaggg gtgcgcgtcc atggcttccc cgccctcgtc
     2101 gacgaggggg acgccgtcgc cctgcgggtg ctgcccaccc ccgccgaggc cgagtccgaa
     2161 caccgcctcg gggtgcggcg gctgctcctg ctcggcacca caccgccgtg gaaacacgtc
     2221 ctcgggcggc tcaccaacgc ccagaaactc gcgctcggcc acaatccaca cggctcggtc
     2281 ccggcgcttc tcgatgacgt cctcgcggcc gctgtggatg cgatcgcggc cgaacgggtc
     2341 catcacgtcc cgcgcaccgc cgccgagttc gcggccgccc aggacgcggt ccggacccac
     2401 ctgggcgctc gggtcgtggc cgcggtcggc ctcgtcgagc cgatcctcgc caagcatctg
     2461 cagatcgtgc gccgcctcga cggcatgagc gcgccgcacc tggccgacct gcgtgcggac
     2521 gtgcgagccc aactcgctga gctcatccgg ccgggcttcg tcgccgacac cggcctggcc
     2581 cggctgcccg acctcgaccg ctacctgcgg gccgtcctgc atcgcctgga caaggctccg
     2641 ctggatctgg cccgcgacag ccagcggctc accgaggtgc tcgacgccga ggccgggtat
     2701 gccgcgctgc tggcttcgct gcgcccctcc caacgggggg cggcccaggt cacggacatc
     2761 gcgtggatga tcgaggagct gcgggtgtcg ctgttcgcgc agagtctcgg gacggcatac
     2821 cccatctcgg tcaagcggat cgagaaggcg atcggcgcga tcccgcgcga ctgacgccgc
     2881 tggtcagcag cgttagcggc gaccgaccgc caggaagccc ccgatgacca cccagaagac
     2941 gaacagcacg aggaacccga tcacggcggc gaggatgcag ccggcggcgc cgccacggta
     3001 gggagtctgc ggagtgaggc cgaggcggcc aggcaggttc tccgacaagg gggcggcgta
     3061 atagacctcg gtggtcactc ctggctggac gtccacggcg atgtcgccct ggccgaaggt
     3121 ggacccactg acgttgacct cgcacgagag catgtgccga ccgggcgcca cgggatagac
     3181 gttgcggccc gcggcattga ccatccggac gccgtccagc aggacgttcg gtttgatcga
     3241 cacgccgatc gtccacggca ggctgatggt cacggcgatc tcgcccgatg ccggggcggg
     3301 gagggcgtcc tgcggagtgg tcatgggttc ccctgagctg agccgacgaa acgctcccag
     3361 cctagagccg cccctccctc gcttcgcggc ggcccgctcg gaatggctcg ggcgccctgc
     3421 gtgttgacgc gacatcagca cttgggagga tgagtacgtg caagacccga cagcgctcta
     3481 ccgattcgag aacgacgccg accccgccga ggtgagggct ggtgtgctcg ttgctgccct
     3541 cggcggtttc gtcgatgccg ggggcaccca gaggttgctc accgcacatc tcttggagaa
     3601 ccactcgtcc caggttgtcg cgagtttcga cgtcgaccag ttgctcgact accgcgggcg
     3661 gcgtccgacg atgaccttcg acaaggatcg ctggaccgcc tatgacgacc cgtcgctggt
     3721 tctgcaccgc gtggtcgatc gggccggaac gccgttcctc ctgctcgccg gtcccgagcc
     3781 cgactaccag tgggagcggg tcaccgccgc gatcctgcag ttggttgcag ccctcggcgt
     3841 ccagttgacg gtgagcgtgc acggcatacc catggccgtc ccgcacaccc gcccgctggg
     3901 cacgaccccg cacgcgacca ccggacgcct cctcggcgag gtcgagcccg tattcgggac
     3961 gatccaggtg cccgggagca tcgccggact gctcgaactt cgcctcggcg aggcgggccg
     4021 cgacgccatg gggtatgccg tccacgtccc gcactacctc gcccaggccg acttcccgga
     4081 cgcctcgctc gtggccttcg acgccctcgt cggcgcgacc gggctcgatc tggcgcgcga
     4141 cgagttggcg acctcggcgc tacacacccg cgagcaggtc gccacggaat tggcggacaa
     4201 cgacgaggtc cggacgctcg tcgtgtcgct cgagcggcag tacgacgcgt tcatggaggg
     4261 ccgcacccga ccgagcctgt tggcgaccgg aatgggcgaa gtaccttctg ccgacgagat
     4321 cgccgccgat gtcgaggcgt tcctgcgcga ggtcaacgac gaaggccgcc aggcctgatc
     4381 gacccgatct gcggagtgtt ggccacatcg gaatgtggct aacactccgc agatcgcgat
     4441 cgggctgagg cgggggcgat cagcccttgg cttcgtccca gcggttgatg atcgacacgt
     4501 cggcgacgaa acgcaccggc ggcccggcgc tcgcgccgac ccagcgtcgg gagctgtcgg
     4561 tcaacgtctg atgcacgagg gcggcaacgg cctcggaggc ctcgacgggg acgtgcagga
     4621 gcaactcgtc gtgcaggcac aggacgatct ggcccccgag cgaggggaga tggtggcgca
     4681 ccgtcgccgc ccacgccttg aacaactcgg cggcggcacc ctggatgatc gcgttgcgag
     4741 cgaagcgccc tcgagccgca tcccacccct caccgaccac ctgcccggcc tcgggggcca
     4801 acgccgagtc cagccggatc agccgccccc cgaaggtgcg cagggggcgg cgagccagcc
     4861 ccgcggcata cgcctcatcg agcaacccca tcgcgaccgg ataggcgcgc tgcaggtcac
     4921 gcagcgcctc gccggcggca cctgagcgct ggccgtacat cgcggcgagc accgcgatct
     4981 tggccaccga ccgctcgacc cgcagtttcg ccgcgaccgg cgcgtagagg tcgtccgcga
     5041 gcgtcgcctc ggcgaacgcg gcgtcgcccg acaccgccgc gagcacgcgc ggctcgatct
     5101 ggcccagatc ggcccgcacg aagacgaatc caggctccgc ggcgacagcg ggccgcaacg
     5161 ggaccggaag gttgtgcagc ccagccgagg cggtcatccg ccccgccgcg ccgtcgctcg
     5221 ccgtccacgc gccgcgcagc cggtcgtcat gccccacctg ctcgtcgagc cagcgatagc
     5281 cgtaggtcgt ggcgatccgc tcgtccttgc gccattccag gagcgcggcg acgatcggat
     5341 gggtcgcgcg gtagggctcc aacacccact tgcgggtggt cgcgacctcc actccggccg
     5401 cggcgaggag gtccttgacc tgggccgggt tgcgcaggtc ggctcggcga cccggagcgt
     5461 gacgcaggac gagcgcgtcg cgctcgccgc ggatccgcgc ggcatcggcc tccgacgtcg
     5521 ggcgtgcccc ggccgcagcg gcgaccagcg cctcggcccg cggccggtcg atcggcaggc
     5581 cgtcgcgctc taactccaga cacagcacgg ccgcggccga ctcggagtat gccgtcgcca
     5641 ccgcccgcgc gccgaccgag ccgaggcgct cgcgctggct gccagccagg tcgcccacaa
     5701 aggcggccaa cgcaagcagg cgcgcgtcgc ggtcggccca cgccggggtg aaggcatctg
     5761 cccgcaggta gccgtcgggg ccgatcggag cgtcggggtg ctcatcggcc agcgggtcga
     5821 ctgcgagatc gaacaggtcg gcctcgcgcg cctgcgggat gccctcgggc gccagaccgc
     5881 gcaggacagc gtggaccagc cccgggcccg ctgcccaccc accgtgcagg atgcggtgcc
     5941 cctccgcgag gtcccagctg cgggccgggc cgacaccggc atccaccagc gcgcgcagac
     6001 tcgtcgcggc gctcggcacc gcccatcggg ggtgttcgtc ggcctccagc cgggcggcgt
     6061 ccgcgagcac gtcgcgcgcc gagccgacgc gcggcatacc ggagtgcagg tatgccgccc
     6121 gctcgcccac gacgacgagc gggacgagct cgtttccggg ggcgaccatc cccccatcct
     6181 cgcgcagccg ttcgggacgg acgtccgatg ccgcgtgtca ggggcgtggg cgacggcgtg
     6241 gggcgaggcg ggcgcgccgg aagttgcatc gccggaccgg cgcggaaagg atgaccgcag
     6301 cagtggcgcg gccgtcggct gcgcgcgggc ggaaggacag cgtcgtgggg acattcggca
     6361 agggcgagtc gagcgagttg tcgagcgtgc ctcggggcgc tctcgaacgg gccgccgaca
     6421 gcgagtcgga ggggggcctc accaaggtgg ccaccgagtt ggtcgagaaa ctgctcgagg
     6481 tcgggattga cggccgcggg ccggtcaagt cggcgcacga ggtcgccgtg gccgcgcggg
     6541 cagaggttcg cagcgatgac gccgcgatca aggtgatcat ccgcaagcac gtccgggccg
     6601 ccgccgtcgg agggttcatc acggggttgg gcggattcgt gacgatgccc gtcgccttgc
     6661 cggccaacgt cgcggagttc tacctcatcg ccacccgcat ggtcgcggcg atcgccgacg
     6721 tgcgcggata cgacgtcacc gatccgcaga tccgcacggc agtcctgctc accctcgtcg
     6781 gcgtcgatgc caatgaggtg gtcaagaagg cgggagcgat tcccggggtg tccttcggcc
     6841 cgggcggcac cctcaccgcc cttgcgtccc agcgcctgcc cggccccgcc ctcatggtgc
     6901 tcaacaaggc gatcggcttc cggttggtcg cgcaggccgg tcgcacgctg ctgggccgcc
     6961 tcggtaaggc ggtgcccgtc gtcggcggtg ccatcggcgc gggggtcgac ggctacctgc
     7021 tgagccggat cgccgacggc gccacccgcg agttcccgcg ccgcgccgac tgagccccgt
     7081 cacgggccgg cggaatctcc ggccggtccc ggtcgttgag gtgaccgatg ggtcgttcgg
     7141 cccgtcacag gctcggtccg cgaggagtat tgaggacatg gctgctgcac ggggggcgag
     7201 ctcggctcgc tgggggatga ttcgggcctt ggggctcgca ctacgggccg cgacccggcc
     7261 gggcgcgccg gggatgggtg aacggctctc ggcgctgcca cgcatggtgc gcgcggcgat
     7321 gcgcggcgag tatgccggtg tcacccgagg gcgactgctg gggatgctgg gggcgctgct
     7381 ctacgtcgtg tcgcccgtcg atctcgtccc agagagcctc ttcacgatct tcggcctggc
     7441 cgatgacgcc ctcttggtca cctggctggc ctcgtcgctc gtcaacgaca cggaggcgtt
     7501 cctcacctgg gagcgtgggt tgtcgcgcgc cgggtcgacc tcggcggccg gtggcccgca
     7561 ggatgccccc gccggcgagc gcaccactcc ggaagactcg tcggcacact ggaccgttca
     7621 gtcgcacgtc gtgcgctgac gtcgccgggc aggcgcggcc agcggttgtc cacacccccc
     7681 tcgaccgtcg tcacatcgtc cacagccatt cgggtgatcc cgctggctgg gggcgaacgc
     7741 ggccacgatc gtcctcatga cgacgacgat tcgcatcacc gacccggtcg acctcgtgaa
     7801 ggtcgtcccg tatcagttgg gttatcaccc ggagcgttcc ctcgtgctga tcggcttgcg
     7861 ccgcaagcag atcgggctgg tgcaacgcct cgatctgccg acgctgccgc gtgactgcga
     7921 ggcggccgcg gacctcatgg ccgggcatct gcgcaaggac ggctgcaccg gcgccgtcct
     7981 tgtgctttac gaggacaccg aaggcgaggg ggcagtcgcc gccgccgcct tggtagcccg
     8041 tctgcaggtg gggcgcttcg acctcgtgga acacgtggtc gtccgggacg gccacgtgta
     8101 cttcggggcg ctggccgggc gcgccgaccg gcccgtgagc ggcgtcctcc ttccgccgga
     8161 cgaccaggtg ccggccgtcg cggactacgt cgcccacggg cgccgacccg ccccctcacg
     8221 cggttccctc gatgagcgac tggctcccga agacgacgct ttgagcgagc gggtgcgcct
     8281 cgccgcggcg cggctggggg tgctgcggcc cgtcctcggc agccggccga aggcgatggc
     8341 cgactggggc gccttcctcg acgtggggga tgacgcctgg ttcgccggga cgcttccatc
     8401 ggcggcggcc accgcccgga tgtgtcacag cctcagggat ctgcaccttc gtgatctggt
     8461 cacggcctgg ctctgccccg gaacccttga gttgagcgcc ttcgacgagg acctgcgagc
     8521 gctcgcggtc gagcacctgc ccgccgggcg cgcctggccg gaggcctacg cggctgcagg
     8581 ccacagcccc gcggccggag gggtcgccga tgatgcaccc gctgcccgac cgcgcggtgg
     8641 cgccgggtct cggcgacggc gagccgcctc gtcgaagcct cggcgggtcg tcacggcagt
     8701 ggcgggcccc cggaccgacg aggacgaccg gctggtcgag aggctgtgct ggctggcgcg
     8761 gcatacccct gaggtccatg cgagcgcggt gctgagcgtc ctggcgtccg tgacctggca
     8821 cctgggtgac ggcaccctca cgcgggtcgc cctcgaccgg gcgctcgccc tggaccccgg
     8881 ctatcggctg gccctgctca tcgagcggat ggtcgacctc gccatccgac caggccgcgc
     8941 cctctcgtcc acgggatgaa ccggtcgtgg caggccgccg cggcggcggc atagactcgg
     9001 gacaggtcat gagtgtcagc gacaagcccc ggctcgctga ccggcaaccc ttcttcgtga
     9061 tggggtgccc cgggtgaaga cccggcccgc gcgtcccgct cgggcaagcg cgagcgtgag
     9121 gagtttcgtc cgtgaccgtc agcgacgacc cgccgttcgt cacgggcgcg tggcgcgacg
     9181 gagacccggt cgggcacaga gctttcgttg acttccccga actgcacctt gagcggggag
     9241 gcgtcctgcc gtcggtccga gtcgcctacg agacgtgggg gacgctgaac ccggcgggaa
     9301 gcaacgccat cctcgtcgaa cacgccctca cgggggacag ccacgtcgtc ggtgctgctg
     9361 gaccggggca cgcatccccc ggctggtggg agggcctggt cgggcccggt cgcgacatcg
     9421 acaccgacga gtggttcgtc gtcgcggcca acgtcatcgg tggatgccag ggcagcaccg
     9481 gccccgcctc gcccgccccg gacggtcggc cctgggggag tcggttcccg ttcgtgacga
     9541 tccgcgatca ggtggctgcc gaggcggctc tggccgatgc cctcgggatc gaggcctggg
     9601 ccctggtcat cggcggatcg atgggcggga tgcgggtcat cgagtgggcg gtgacctacc
     9661 cggaccgagt ggcacaagcg gtgatcctgg cgagcactgc ggcggcgacc gccgaccaga
     9721 tcgcctgggc tcagccacaa ctgctcgcca tccggtccga cccggacttc gccgggggcg
     9781 actactacgg ccacagtcgg gggccggacg tcgggctggg gatcgctcga cggattgccc
     9841 aggcgacgta tcgcagcgcc tccgagttgg acgagcgctt cggtcgggaa ccgcagtccg
     9901 aggaagaccc cctcggcgg
//
LOCUS       DAOMZY010000100         9901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_6462_length_9901_cov_6.279403, whole genome shotgun sequence.
ACCESSION   DAOMZY010000100 DAOMZY010000000
VERSION     DAOMZY010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9901
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6462_length_9901_cov_6.279403"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="PLK64_07260"
     CDS             <1..1248
                     /locus_tag="PLK64_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPV79579.1"
                     /translation="TALAVASGARTQMYSLVAAFLMIIVLFVAGPLIANLPQAALAAV
                     VFYAASKLINVKEIKRLARFRKQELLLAMAATVGTVFFGVVTGIGIAIALSVLEMTQR
                     LARPHDGVLGRVPGLPGMHDVADYPTAQTLPGLIVYRYDAPLFFANVGDLRRRATMVV
                     DQENKAFPQSPARWFILNVEANVEVDLTAADGLAELQRDLAERGVRLGLARVKQDLLL
                     PLQRAGLMELIGTDMLFPTLPVAEKAYLIWSAQNPFTPPEPLETESADDDVPVWNVYR
                     FSEEAASATVPAEGLVSKATHAALARAQQAAAAMTSAITSHSDDSSPDADADADADAE
                     PETDADAAGAMLSPVTSSTRDEDSDADEPEADGSESEADLNAASNGTVKGKKGKKGNA
                     TDGNPSNGAVNGILTGARTHAGS"
     gene            complement(1271..2032)
                     /locus_tag="PLK64_07265"
     CDS             complement(1271..2032)
                     /locus_tag="PLK64_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HPV79580.1"
                     /translation="MGLSDVLYGVYERRLAHDLDRSNVPRHVGVMLDGNRRWAKANGF
                     DTVGGHQAGADKIQDLLAWCEETGVELVTLWLLSTDNLDRDSSELEPLLGIIEEAVND
                     LAAAQRWRLHPIGALDLLPEATATALLAAETATAGVTGLTVNVAVGYGGRREIADAVR
                     QLILEQAATGIPIEEIADKVSVEAIAQHLYTRGQPDPDLVIRTSGEQRLGGFLLWQSA
                     KSEFYFCEAYWPDFRRVDFLRALRAYANRERRFGR"
     gene            2305..2976
                     /locus_tag="PLK64_07270"
     CDS             2305..2976
                     /locus_tag="PLK64_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HPV79581.1"
                     /translation="MSAIQLPEHHVAKPRLRGWLHAGTFPVAVIAGLVLIILAPAGRA
                     RIGAVVFVITAAMLFGTSAVYHRGNWSQKVHQVLKRLDHSNIFLIIAGSYTPFALCLL
                     PTEQARTLLITVWAGALGGVAFRVLWVDAPRWLYTPIYVALGWVAVFYFTPLLAGGGA
                     AVMSLIVAGGVLYTVGAVVYGTKRPNPSPRWFGFHEVFHACTVAAFITHFVAVSLAAY
                     TVATA"
     gene            complement(3014..3304)
                     /locus_tag="PLK64_07275"
     CDS             complement(3014..3304)
                     /locus_tag="PLK64_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79582.1"
                     /translation="MGGPVPNSAVGPGLGAFLAFFALAVALYFLMRNMNARMRRMAYR
                     ERDRIAALEAAEAAKSGTSTPADSGPTATPSDAAAQGDAAAPKPKRKKPNEP"
     gene            complement(3305..4282)
                     /gene="mca"
                     /locus_tag="PLK64_07280"
     CDS             complement(3305..4282)
                     /gene="mca"
                     /locus_tag="PLK64_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012802202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010126 - mycothiol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol conjugate amidase Mca"
                     /protein_id="HPV79583.1"
                     /translation="MPPTPESAPAPDTGQRRDGFRLLAVHAHPDDESSKGAAASAMYV
                     ADGARVCVVSCTGGERGDVLNEALKDDPHIQRDIAQVRRDEMAAAAAALGVEHVWLGF
                     VDSGLPEGDPLPPLPEGCFALEPISVTTEALVRVIREFRPHVVTTYDEHGGYPHPDHI
                     MTHTVSMSAFAAAGDPAAYPHAGQPWQPVKLYYNQTHSGGRIRAFHEALLAEGLDSPY
                     GEWVEHIERRGQRHVTTRVPCAEFFAARDRALLAHATQVDPEGSWFKMPRELERSVWP
                     TEDFEAALSYVPIIEVEDDLFAGLPSVAEADDMATRQGLTLVFDGRVAV"
     gene            4357..4728
                     /locus_tag="PLK64_07285"
     CDS             4357..4728
                     /locus_tag="PLK64_07285"
                     /inference="COORDINATES: protein motif:HMM:NF025520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4307 domain-containing protein"
                     /protein_id="HPV79584.1"
                     /translation="MSSQRPRITPGTGKWWALGIAFTLAFTGFASWKVLSVADQAIDV
                     TLTAVSVVDDRTTVVTFDLHRPPAQAVVCTVQAQDQRKAVVGTVTVDLPPATERTTTH
                     QVTVKTTTRAFTGLVHDCVRA"
     gene            4813..5997
                     /locus_tag="PLK64_07290"
     CDS             4813..5997
                     /locus_tag="PLK64_07290"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HPV79585.1"
                     /translation="MTTQRPRGFSTTAIHAGQEADESTGAVVTPIYQVSTFKQDGVGG
                     LRGGYEYSRSANPTRAALEECLAALEGGSRGFAFASGLAGQDCVVRSLVGPGDHLVVP
                     TDAYGGTYRYVNRVARAHGVEHTVAPMADVAAVQAAIEPGRTKLVWVETPSNPLLGVA
                     DIAALAAVAHEAGALLVVDNTFATSYLQQPLALGADIVTHSTTKYAGGHSDVVGGAVV
                     VAQEVGVAAYADAAERIAFHQNAIGAVSGPFDAWLVLRGLKTLAVRMERHCDNAERIV
                     EFLSGHDAVSQVLYPGLAGHAGHEVAARQMRRFGGMVSFRLRRGEDAAVKACAAVSLW
                     TLGESLGGVESLIEHPGRMTHASVVGTALEVPGDLIRLSVGIEDVEDLIDDLREALDI
                     HG"
     gene            5997..7370
                     /locus_tag="PLK64_07295"
     CDS             5997..7370
                     /locus_tag="PLK64_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HPV79586.1"
                     /translation="MGSPIGHQALLVVDVGSTFTKAALVQVSADSAGDETGAVLGRAE
                     TPTTLDTDVMDGVRSLAAHLGDAAAVDDGRIVACSSAGGGLRLAVIGYERTVTAEAGY
                     RVGLSAGAKVVHVASGPLDTVALAALAEARPDIVLLVGGTDGGNAEVLQHNGIALAAS
                     TLPHDVPVVVAGNLDAQPAVAAALQRAGRPHVLGDNVLPEIGVVAPASARVAIREVFL
                     RHVIGGKGLSTDAAFARVVRAATPDAVLAGVEVLAEVLDQDVLVVDVGGATTDVYSAV
                     TPRGDDAGRRTEVVAPLRFSRTVEADLGMRWNAEGVIEAAAREHLPTSTALRRYAARL
                     GEQPAYLPSGPGEAALDLDLARLAAIVAVRRHARPPFPGEAPRALTDLGLVLGSGGVL
                     RHTPSTEARALLEAVAADHAGGWRVPRAPRIGVDAAYLLVVVGLLAPTHPDAARAVAR
                     RLALGDG"
     gene            7382..8599
                     /gene="ilvA"
                     /locus_tag="PLK64_07300"
     CDS             7382..8599
                     /gene="ilvA"
                     /locus_tag="PLK64_07300"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HPV79587.1"
                     /translation="MESLPVTLADVRAARELLAGVVTTTPLEHSRGLSERVGVPVYLK
                     CENLQRAGSFKIRGAYTRMSRLSEAERVRGVVAASAGNHAQGVALAAQLLGISATVFM
                     PVGAPLPKLAATRAYGATVQLHGDTLDEALVAALEYAASTGAVLIHPFDHPDIVAGQG
                     TCGLEILEQLPDVKTILVSTGGGGLVGGIAVAAKSLRPDVRVIGVQAEQAAAWPLSLA
                     AGTPVAVDSMQTMADGIAVGRPGPVPFEVVSNLVDDMVTVSEAALSRAVVFLLERAKV
                     VAEPAGAAAVARLLEGPGPLEGPVVAVVSGGNIDPLLLLRIVRHGLTAGGRFLQVTVR
                     VPDRPGSLARLLADLAATTANVVEVNHVRTDSGLAVDEVEIAVDLETRGTAHREEVLR
                     ALRSAGYRLVFGS"
     gene            complement(8615..9106)
                     /gene="greA"
                     /locus_tag="PLK64_07305"
     CDS             complement(8615..9106)
                     /gene="greA"
                     /locus_tag="PLK64_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPV79588.1"
                     /translation="MTETAKSAYLTPEAHDRLKGELEHLSTEGRVELSKKIEAARLEG
                     DLRENGGYHAAKEEQGKMEARIRQLTQLLESAIVGETPPPDDGIVEPGMVVTVEMFDE
                     EETFLLGHREIQDHTDLHVFSEASPLGRAVNGRTVGETVSYVAPNGKTIEVKILNTSP
                     YAG"
     gene            complement(9287..9877)
                     /locus_tag="PLK64_07310"
     CDS             complement(9287..9877)
                     /locus_tag="PLK64_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79589.1"
                     /translation="MTRRLIQLYAGLVTFAFGEALILEAGLGVIPWDVFQQGLVNHWG
                     LTMGTWSIIVAVAVLLLWIPLRQRPGIGTVSNAIVIGAALDPMLRAIEAPESLGWRAA
                     YLVAGILINGVATAAYIGARLGPGPRDGLMTGLVRLTGRSVRLVRTGLEVTVVLIGWA
                     LGGNLGLGTVLFAVSIGPVVHVFLPKLTVPGGSALH"
BASE COUNT         1394 a         3608 c         3356 g         1543 t
ORIGIN      
        1 acggcgctcg cggtggcgtc cggcgcacgc acccagatgt attcgttggt tgcggcgttc
       61 ctgatgatca tcgtcctgtt cgtcgccgga ccgttgatcg ccaacctgcc gcaggctgcc
      121 ctggccgctg tggtgttcta tgccgccagc aagctgatca acgtcaagga gatcaagcgg
      181 ctggctcgct tccgcaagca ggagttgctg ctggcgatgg ctgccaccgt cgggaccgtc
      241 ttcttcggcg tcgtcacggg gatcggcatc gctatcgccc tcagcgtcct ggagatgacc
      301 cagcgtctgg cccgccccca cgacggtgtg ctcgggcgcg tcccgggcct gcccggcatg
      361 cacgacgtcg ccgactaccc gaccgctcaa acgctcccgg gcctgatcgt ctaccgctac
      421 gacgcaccct tgttcttcgc caacgtcggc gacctgcgcc gtcgggccac catggtcgtc
      481 gaccaggaga acaaggcctt cccccagagc cccgcgcgct ggttcatcct caacgtcgag
      541 gccaacgtcg aggtcgacct caccgccgcg gacgggctgg ccgagctgca gcgtgacctc
      601 gccgagcggg gcgtgcgctt gggcttggca cgtgtcaagc aggacctcct gctccccctg
      661 cagcgggccg ggctcatgga gctcatcggc accgacatgc tcttcccgac gctccctgtc
      721 gccgagaagg cctacctcat ctggtcggca cagaacccgt tcacgccgcc ggagcccctg
      781 gagaccgagt ctgccgacga cgacgtcccc gtgtggaacg tctatcggtt ctccgaagag
      841 gctgcatcgg ccacggtgcc tgcggagggc ctcgtctcca aggccactca cgccgcgttg
      901 gcgagggccc agcaggccgc cgcagcgatg acctcggcca tcacctcgca cagcgacgac
      961 tcgtcgcccg acgccgacgc cgacgccgac gccgacgccg agcctgagac tgacgccgac
     1021 gcagccggtg ccatgctctc cccggtcacg tccagcacca gggacgagga cagtgacgcc
     1081 gacgagccgg aagcggacgg gtcggagtcg gaggccgacc tgaatgccgc cagcaacggc
     1141 accgtgaagg gcaagaaggg caagaagggc aacgccaccg acggcaaccc gagcaatggc
     1201 gcggtgaacg gcatactcac cggggctcgc acccacgcgg gctcctgacc gcgcgcgctc
     1261 gggtgaggtc tcaacggccg aagcggcgct cgcggttggc gtaggcccgc agcgcgcgca
     1321 ggaagtcgac ccgccggaag tccggccagt aggcctcgca gaagtagaac tcgctcttcg
     1381 cgctctgcca gagcaggaag cccccgaggc gctgctcgcc ggaggtgcgg atcaccaggt
     1441 cgggatcggg ttgtccgcgc gtgtagaggt gctgggcgat cgcctcgacg ctcaccttgt
     1501 cggcgatctc ctcgatgggt atgccggtcg cggcctgctc aaggatgagt tgccgcacgg
     1561 cgtcggcgat ctcgcgtcga ccgccgtaac ccacagcgac gttgaccgtg agaccagtga
     1621 cgcccgccgt cgcggtctcg gccgccagta gtgcggtggc cgtcgcctcc ggcaacaggt
     1681 caagggcgcc gatcgggtgg aggcgccagc gttgggccgc ggcgaggtcg ttgacggcct
     1741 cctcgatgat ccccaggagc ggctccaact cgctggagtc gcggtcgagg ttgtcggtcg
     1801 agagcagcca cagggtgacc aactcgacgc cggtctcctc gcaccaggcg aggaggtcct
     1861 ggatcttgtc ggccccggcc tgatgtccgc ccaccgtgtc gaagccgttg gccttggccc
     1921 agcgccggtt gccgtcgagc atgacgccga cgtgccgcgg cacgttcgac cggtcgagat
     1981 cgtgcgccag ccgacgctcg tagacgccgt agagcacgtc gctcaggccc acccgactcg
     2041 ccttccgttc gcgaacctcg ccgttgtcgg ctgttcccga cacgctactc ccgtatgccg
     2101 cgtcgctccc atcccccgcc cgcaccccga aacccccgcg aaccccctga ccccggagat
     2161 agcgctcgca cggtcatctt tacgcccccg cgaaccccct gaccccggag atagcgctcg
     2221 cacggtcatg tatgccgcga gccttgcctc acactcgtaa cttacggagt cgtaagttac
     2281 ggcaacgtaa gttatccttg actcatgagc gccatccagt tgcccgagca ccacgtcgcc
     2341 aaaccccgcc tgcgcgggtg gctgcacgcc ggcaccttcc ccgtcgcggt catcgcaggc
     2401 ctggtcctca tcatcctcgc ccctgccggc cgagcgcgga tcggagcggt ggtcttcgtc
     2461 atcacggccg ccatgctctt tggcacctcg gcggtctacc accgcggcaa ctggtcgcag
     2521 aaggtccacc aggtgctcaa gcgcctggac cactcgaaca tcttcctcat catcgccggg
     2581 tcctacacgc ccttcgccct gtgcctgctg ccgaccgaac aggctcgcac cctgctgatc
     2641 accgtgtggg ccggcgccct cggcggcgtg gccttccggg tgctgtgggt cgacgcgccc
     2701 cgctggctct acacgccgat ctacgtcgcg ctgggttggg tcgccgtctt ctacttcacg
     2761 ccgttgctgg ccggcggcgg ggccgctgtc atgtcgctca tcgtcgcggg cggcgtcctc
     2821 tacaccgtgg gtgcggtcgt ctacggcact aagcgtccca acccctcacc gcggtggttc
     2881 gggttccacg aggtcttcca cgcctgcacg gtcgccgcgt tcatcacgca cttcgtggcc
     2941 gtctcgctcg cggcatacac cgtcgcgacg gcctgacccc gacggcgagc ccgttcgctc
     3001 agggctcgtt cgctcagggc tcgttcggct tcttccgctt cggcttcggt gcggccgcat
     3061 caccctgggc cgccgcatca ctcggcgtcg ccgtcggccc cgagtccgcc ggcgtcgagg
     3121 tcccagactt cgccgcctcg gccgcctcca gggcagcgat ccgatcgcgt tcgcgatagg
     3181 ccatccggcg catccgcgca ttcatgttcc gcatgaggaa gtagagcgcg accgccagcg
     3241 cgaagaacgc gaggaacgcc ccgagccccg gcccgaccgc cgagttggga actggaccac
     3301 ccatctagac cgccacccgt ccgtcgaaca ccagcgtcaa accctgacgc gttgccatgt
     3361 cgtccgcctc cgccaccgac ggcagcccgg cgaacagatc gtcctccacc tcgatgatcg
     3421 gcacatagga cagcgccgcc tcgaaatcct cggtcggcca gaccgaccgc tccagctcgc
     3481 gcggcatctt gaaccacgag ccctcggggt cgacctgcgt cgcgtgggcg agcaacgccc
     3541 ggtcgcgagc ggcgaagaac tccgcgcacg gcacccgcgt ggtcacgtgc cgctgcccgc
     3601 gtcgctcgat gtgctccacc cactcaccgt aaggcgagtc caacccctcg gcgagcaagg
     3661 cctcgtggaa ggcccggatc cgcccgcccg agtgggtctg gttgtagtag agcttcaccg
     3721 gctgccacgg ctgccccgca tgggggtatg ccgcggggtc gccggccgct gcgaaggccg
     3781 acatcgagac ggtgtgggtc atgatgtggt cggggtgggg gtagccgccg tgctcgtcgt
     3841 aggtcgtaac gacgtgcggt cggaattcgc ggatgacccg cacgagcgcc tcggtggtca
     3901 ccgagatggg ctcgagggcg aaacaccctt cgggcaacgg cggcaacggg tcgccttcgg
     3961 gcagaccgga gtcgacgaac ccgagccaga catgctcgac tccgagagcg gcggccgctg
     4021 cggccatctc gtcgcggcgc acctgggcga tgtcgcgctg gatgtgcggg tcgtccttga
     4081 gcgcctcgtt gaggacgtcg ccacgctccc cgccggtgca ggacacgacg cagacccggg
     4141 ctccgtcggc gacatacatc gccgacgccg cagcgccctt gctcgactcg tcgtccgggt
     4201 gagcatggac ggccaggagc cggaagccgt cacgacgctg cccggtgtcg ggtgcgggcg
     4261 cgctctcggg ggtgggcggc atacctgaat cgtgtccttc gcagggaggg ggttacggtg
     4321 gaccatcatc ccaccgttcg gcggaaggct taccccgtgt cgtctcaacg tccgcggatc
     4381 accccgggga ccggcaagtg gtgggcgctg ggcatcgcgt tcaccctggc gttcaccggg
     4441 ttcgcctcgt ggaaggtcct gtcggtggcc gaccaggcca tagacgtgac gttgacggcg
     4501 gtgtctgtgg tcgacgaccg gacgacggtg gtcaccttcg acctgcaccg gccacccgca
     4561 caggccgtcg tctgcacggt ccaagcccaa gaccagcgca aggccgtcgt gggcacggtg
     4621 accgtcgacc tgccccccgc gaccgagcgc acgaccaccc atcaggtgac cgtcaagacg
     4681 acgacccggg cgttcaccgg tctcgtgcac gactgcgtcc gcgcctgatt cgccaacgcc
     4741 gagccgtcgc ctatccgtcg ctcagcccgc ttgccaccca cgaccggccg cgcggggcta
     4801 aagtcgctcg ccatgaccac gcagcgcccc cgcggcttct ccacgacggc catccatgcc
     4861 gggcaggagg ccgacgagtc caccggcgcg gtggtgacgc cgatctacca ggtgtcgacc
     4921 ttcaaacagg acggcgtcgg cgggctgcgc gggggctatg agtactcccg ctcggccaac
     4981 cccacgcgcg ccgccctcga ggagtgcctc gcggccctgg aagggggctc gcgcgggttc
     5041 gccttcgcct ccgggttggc ggggcaggac tgcgtcgtcc gctccctcgt cggtccgggt
     5101 gaccacctcg tggtccccac tgacgcctac ggcggcacct accgctatgt caaccgggtc
     5161 gcccgggcgc acggcgtcga gcacaccgtc gcccccatgg ccgatgttgc ggcggtccag
     5221 gcggccatcg agccggggcg cacgaagctc gtctgggtcg agacgccctc gaacccgctc
     5281 ctcggcgtcg ccgacatcgc cgcgctcgcc gcggtggccc acgaggccgg cgccctgctc
     5341 gtcgtcgaca acaccttcgc gacgagttac ctccaacagc cgctcgcgct cggtgccgac
     5401 atcgtcaccc acagcacgac gaagtatgcc ggtgggcaca gtgacgtcgt cggcggcgcc
     5461 gtcgtcgtcg cgcaggaggt gggtgtcgcg gcatacgccg atgccgccga gcggatcgcc
     5521 ttccaccaga acgcgatcgg agccgtttcc gggcctttcg acgcgtggct cgtgctgcgc
     5581 gggctcaaga ccctcgccgt gcggatggag cggcactgcg acaacgccga gcgcattgtc
     5641 gagttcctca gcggccacga cgccgtgagc caggtgctct atcccgggct ggccggacac
     5701 gccgggcacg aggtcgccgc ccggcagatg cgccggttcg gcggcatggt gtccttccga
     5761 ttgcgccgcg gtgaggacgc ggcggtcaag gcctgcgccg ccgtgtcgct gtggaccctt
     5821 ggcgagtcgc tcggcggcgt cgagtcgctc atcgagcacc ccggccggat gacccacgcc
     5881 tcggtcgtcg gcaccgcgct ggaggtgccc ggtgacctca tccgcctgtc ggtcggcatc
     5941 gaggacgtcg aggacctcat cgacgatctg cgcgaggccc tggacatcca cggctgatgg
     6001 gctcgccgat cggccatcag gcgctcctcg ttgttgacgt ggggtcgacc ttcaccaagg
     6061 ccgccctcgt ccaggtcagt gccgacagcg cgggcgacga gactggcgcc gtcctgggtc
     6121 gcgccgagac cccgaccacc ctcgacaccg acgtcatgga cggggtccgc tccctcgctg
     6181 cccacctcgg tgatgccgcc gcggtcgacg acggccggat cgtggcctgc tcgtcggcag
     6241 gcggcgggct gcgcctcgcg gtcatcggct atgaacgcac cgtgacggcc gaggccggct
     6301 atcgcgtggg cctctcggcc ggcgcgaagg tcgtccacgt ggcgtccggc ccgctcgaca
     6361 cggtggccct ggcggccctc gcggaggccc gccccgacat cgtcctgctc gttggcggca
     6421 ccgatggcgg caacgcagaa gtgttgcagc acaatgggat cgctctcgcc gcctcgacgc
     6481 tccctcacga tgtccccgtc gtcgtcgccg gcaacctcga cgctcaaccc gccgtcgcgg
     6541 cggccctcca gcgggccggt cggccccatg tgctcggcga caacgtgctg cccgagatcg
     6601 gggtcgtcgc ccccgcgagc gcgcgcgtgg cgatccgcga ggtgttcctc aggcacgtca
     6661 tcggaggcaa gggcctgtcc accgacgccg ccttcgcccg ggttgtgcgc gcggccacgc
     6721 ccgacgcggt cctcgccggg gtcgaggtgc tcgccgaggt tctcgaccag gacgtgctcg
     6781 tcgttgacgt cggaggcgcg accacggacg tctattcggc cgtcacccca cgaggtgacg
     6841 acgccggccg ccgcaccgaa gtcgttgcgc cgctgcggtt ctcacgcacc gtcgaggccg
     6901 acctcgggat gcgctggaac gccgagggag tcatcgaggc agccgcccgc gagcaccttc
     6961 cgacgtcgac cgccctgcgc aggtatgccg cgcgcctggg tgagcagccg gcatacctcc
     7021 cgtcagggcc gggtgaagcg gccctcgacc tggacctcgc ccggctggcg gccatcgtcg
     7081 ccgtccgacg gcacgcccgg ccgccgttcc ccggcgaggc gccccgagca ctgaccgacc
     7141 tcggtctggt gctgggctcc gggggcgtgc tgcggcatac cccgtcgacg gaggcacgag
     7201 ccctgctgga ggcggtcgcg gccgaccatg cggggggctg gcgggtgccg cgagcaccac
     7261 ggatcggggt cgacgcggca tacctgctgg tcgttgtcgg cctgctcgcg ccgacccatc
     7321 cggacgcggc ccgtgccgta gcccggaggt tggccctcgg cgacggatag ccttgcccgc
     7381 catggagtcc ctgccggtca ccctcgctga cgtccgagcc gcgcgggagt tgctcgccgg
     7441 agtcgtgacg accaccccct tggagcacag ccgcgggtta tccgagcggg tcggggtgcc
     7501 ggtctatctc aagtgcgaga acctccaacg ggccggatcg ttcaagatca gaggcgccta
     7561 cacgcggatg tcgcggctgt ccgaggcgga gcgcgtccgc ggcgtcgtgg ccgcgagcgc
     7621 cggcaaccac gcgcagggag tggcgctggc cgcgcaactg ttgggcatct ccgcgacggt
     7681 cttcatgccg gtcggcgccc ccctgcccaa gctcgcggcg acccgcgcct acggcgcgac
     7741 ggtgcagttg cacggcgaca ccctcgacga ggcgcttgtg gcggcgctgg agtatgccgc
     7801 gtcgaccggc gccgtcctca tccacccctt cgaccacccg gacatcgttg cgggacaagg
     7861 gacctgcggg ctggagatcc tcgaacaact gcccgacgtc aagacgatcc tcgtgtcgac
     7921 cggaggaggc gggctggtcg gcgggatcgc cgtggcggcg aaatcactgc ggcccgacgt
     7981 gcgagtcatc ggcgtgcagg ccgagcaggc cgctgcctgg ccgttgtcgc tggccgcggg
     8041 gacgccggtg gccgtcgact cgatgcagac gatggccgac gggatcgcag tcgggcgccc
     8101 cggaccggtg ccgttcgagg tggtgtccaa cctcgtcgac gacatggtca ccgtgagcga
     8161 ggccgcgctg tcccgcgccg tcgtcttcct cctggagcgc gcgaaggtgg tcgccgagcc
     8221 ggccggagcg gctgcggtgg cccgcttgtt ggagggtccc gggccgctgg aggggcccgt
     8281 cgtcgccgtc gtgtccggcg gcaacatcga cccgctgctc ctgctgcgga tcgtgcgcca
     8341 cggcctgacc gccggcggtc gtttcctcca ggtcaccgtg cgggtgccgg accggccagg
     8401 ttcgctggcc cgcctgctcg ccgacctggc cgcgaccacg gcgaacgtcg tcgaggtcaa
     8461 ccacgtgcgg accgattccg gcctggccgt cgacgaggtc gagatcgccg tcgatctgga
     8521 gacccgcggc accgcccacc gcgaagaggt cctgcgagcc ctgcgctcgg ccggctatcg
     8581 gctggtcttc gggtcctgag gtgcgggcgg tggctcagcc cgcgtagggg ctggtgttga
     8641 ggatcttgac ctcgatggtc ttgccgttgg gggccacgta actcacggtc tctccgacgg
     8701 tgcggccgtt gacggctcgg cccagcgggc tcgcctcgct gaagacgtgc aagtcggtgt
     8761 ggtcctggat ctcgcggtga ccaagcagga aggtctcttc ctcgtcgaac atctcgaccg
     8821 tgacgaccat gccgggctcg acgatgccgt cgtccggggg cggggtctcg ccgacgatcg
     8881 ccgactccag caactgggtc agctggcgga tccgggcctc catcttgccc tgctcctcct
     8941 tggctgcgtg gtagccaccg ttctcgcgga ggtcaccttc gaggcgcgcc gcctcgatct
     9001 tcttggacaa ctcgacgcgc ccctcggtcg agaggtgctc aagctcgccc ttgagccggt
     9061 cgtgggcctc cggggtgagg taggcgctct tcgcggtctc ggtcacggtt gttcgtctcc
     9121 ttgcctgtcc cgggcaaagg accagtgcgg ctcggttact cggggtgtcg tcgggtcgcg
     9181 cggcgctgcc aaccaggcgc cgagatcggc gcgctgtcgc agctgccggg gcgcgggccc
     9241 cggtgtgaag ttcacagttt accaagcctg ggcgaaaatg tgcccttcag tgcaatgccg
     9301 atcccccagg cacggtgagt ttgggtagaa atacgtggac gacgggcccg atcgacacgg
     9361 cgaaaaggac cgttccgagg cccagatttc ccccgagcgc ccaaccgatg agcacgacgg
     9421 tcacctccag cccggtgcgg accagccgga cggacctccc ggtcagccgc accagcccgg
     9481 tcatcagccc gtcgcgcggc ccgggcccca gccgtgcccc gatataggcg gccgtcgcca
     9541 ctccgttgat gaggatcccc gccacgaggt atgccgcccg ccaccccagc gactcgggtg
     9601 cctcgatcgc gcggagcatc gggtccaggg ccgcgccgat gacgatggcg ttgctcaccg
     9661 tgccgatgcc cggccgctga cgaagcggga tccacagcag caggaccgcg acggcgacga
     9721 tgatcgacca ggtgcccatg gtgaggcccc aatggttgac caggccctgt tggaacacgt
     9781 cccaggggat gacgccgagg cccgcctcga ggatgagcgc ctctccgaac gcgaaggtga
     9841 ccagcccggc atacaactgg atcagcctgc gcgtcagccg gcgaggtgga ggcgcggccg
     9901 g
//
LOCUS       DAOMZY010000101         9869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_6485_length_9869_cov_6.457815, whole genome shotgun sequence.
ACCESSION   DAOMZY010000101 DAOMZY010000000
VERSION     DAOMZY010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9869
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6485_length_9869_cov_6.457815"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PLK64_07315"
     CDS             complement(<1..625)
                     /locus_tag="PLK64_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79590.1"
                     /translation="MTSTQPPLTPAPTVLEVPTSVGPARVHLRRARGSRGVLLLGHGA
                     GPSITTTDLIATTEAAVGAGWAVALVEQPWLVAGGRVAARPPILDTAWVSVAAALRAR
                     GGALATARGPWLVAGRSAGARVACRTALSVGADAVLCLSFPLHPPGKPESSRAQELEG
                     ALAGGLPVSVVQGERDPFGTPAEVLAAVSLPPTRMYAVPGTHTIPRGS"
     gene            complement(622..1458)
                     /locus_tag="PLK64_07320"
     CDS             complement(622..1458)
                     /locus_tag="PLK64_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HPV79591.1"
                     /translation="MCGRYAAAKDQADLVLEFDVEVVATAEPSRSVLVKPQDPPAGAP
                     DYNVAPSKQAPVVLTRASRSDPEGPLSRQLRLLTWGLVPSWAKDVKIGMRFINARAET
                     ALESGPFARAALARRCLVPADGWYEWQASPTVLDAKGKPRKQPFFIHRGDAAPLAFAG
                     LYEFWRDPAVPDRDDPLAWLTTFTIITTAAEPGLDRIHDRQPLVLEPDNWAAWLDPAL
                     TDPAAVERMLRFERAGRFAAYPVSTAVNGSAGNGAHLLDALPAAALRGVVDPTTGEII
                     GS"
     gene            1536..2906
                     /gene="aroA"
                     /locus_tag="PLK64_07325"
     CDS             1536..2906
                     /gene="aroA"
                     /locus_tag="PLK64_07325"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPV79592.1"
                     /translation="MSIDVDQQLWPAPVAPGPLDAVVRLPGSKSLTNRYLVLAALADG
                     PSRLRAPLRSRDTLLMAAALGTLGADIRDTGALNAVAPESSGERAAYPVSDWVVTPGA
                     VRGGGHLDCGLAGTVMRFLPPVAALADGPVAFDGDAGARRRPMGPVLDALRALGVGVD
                     DDGSGCLPFTVQGRGRDGVPGGEVTLDASASSQFVSALLLAGARYDRGVTIHHDGKPV
                     PSQPHIEMTVQTLREAGVVVDDSDANTWRVAPGSIRGLDVVIEPDLSNAGPFLAAALV
                     AGGTVLVPDWPERTTQAGDLLREILAAMGARVQLGSDGLRVTGSGPIEGIDIDLHEAS
                     ELTPTVAALAALAGSPSWIRGVAHIRGHETDRLAALDTELSALGTQVTQTEDGLRIVP
                     APLRGNRFGTYHDHRMATAGAILGLRVPGVLVEDVATTAKTLPDFTGRWTAMLGASHG
                     GAEGNG"
     gene            2903..3943
                     /gene="rsgA"
                     /locus_tag="PLK64_07330"
     CDS             2903..3943
                     /gene="rsgA"
                     /locus_tag="PLK64_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPV79593.1"
                     /translation="MSWDHLDESDVRVRPGRGSRPRTKDRPAHGDALVGTVVTVDRGR
                     YGVLMAAGVGGRKVPERMVVAMKARELGRKGIVVGDDVGLVGDSSGHPDALARIVRVE
                     PRRTVLRRSADDTDPIERVLVANADQLVVVSSLTDPPPRPRLIDRCLVAAYDAGLEPL
                     LVLTKADLVPADDFIGQYAPLAVRCVITSRRPDRTLIGLEDVRAQLAGRVSVLVGHSG
                     VGKSTLVNALVPGADRSVGRVNDVTGRGRHTSTSAIALRLPNDPGWVIDTPGVRSFGL
                     AHVDVARIVSHFPDLEPGTETCPRGCTHDEPECGLDEFVANGGAGASGPARLDSFRRL
                     LRARTGDVDSDA"
     gene            3924..4688
                     /locus_tag="PLK64_07335"
     CDS             3924..4688
                     /locus_tag="PLK64_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPV79594.1"
                     /translation="MSTPTPEDAADRETASAQGSGLAAHGVSGHPSTPTARLVGALPV
                     IVGFIALAIGVTVTQETYESVQSGRGAAAFDEPVQTWMVAHRTPWLDTVATWFTHLGG
                     KVGMTILATVTVVALARRWRTWSPVLIMLWATAGSLTLTTLGKTLTARARPPAAQAVP
                     PLETSASFPSGHTLNAVLVTSIIGYLVLLHVRSRRVQVLTWCGLALFATLIGLSRIYL
                     GHHWLTDVIAGAAVGGAWAAAVILGHHLYLRLRADP"
     gene            4729..5508
                     /gene="hisN"
                     /locus_tag="PLK64_07340"
     CDS             4729..5508
                     /gene="hisN"
                     /locus_tag="PLK64_07340"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HPV79595.1"
                     /translation="MSYDDDLRLAHVLADAVERVTMARFRAEDLMVETKPDLTPVSDA
                     DRAAEELIRHQLARTRPRDAVVGEEFGTTGHGPRRWIVDPIDGTKNFVRGVPVWATLI
                     ALVDGDEPVVGLVAAPALARRWWAAKGAGAYAGRSLSAARPLHVSGVSALADASLSYA
                     SLGGWSEIDRREDFLDLMDGCWRTRAYGDFWSYMLVAEGAVDIAAEPSLQTYDMAALV
                     PIVTEAGGRFTGLDGRPGCWSGNAVATNGLLHETVLDLIGD"
     gene            complement(5518..6024)
                     /locus_tag="PLK64_07345"
     CDS             complement(5518..6024)
                     /locus_tag="PLK64_07345"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPV79596.1"
                     /translation="MRSALVVDSPIGPLRLESVDGVAVSAIRFDGRPEASDPTSAQAP
                     TAAVLVEAARQLAAYFAGSLRSFDLPLAPHGTDFQRAVWHELALIPYGTTVTYGEVAL
                     RLGLPVSASRAVGAANGANPIPVVLPCHRVIGADGTLTGYAGGLSRKQTLLHLEGVAT
                     ERDQLELF"
     gene            complement(6035..7495)
                     /locus_tag="PLK64_07350"
     CDS             complement(6035..7495)
                     /locus_tag="PLK64_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPV79597.1"
                     /translation="MTGVPQRTRMSAPDALPRPDVLVPQPYELPVPTRFRLANGASGL
                     AYDLPGQYVLSVRLSVPMPLRVEPRDLEGVATIMARTLDEGTARHTSVEFARLLERRG
                     VAIGCGVGDSGLSVELDVAKHHLAYGLDLMHQCLAEPAFPAEEVARQVSTRLAEIEQE
                     RSVASHRVAANSAAARCEAIEQERSVASHRAAAEFAATFYSPVGRSSRPVGGTAETVA
                     AITRDAVVAFHAEHVAPAEATVVVAGDLAGLDVVAELDLSLGRWGTPDTYRSPGPWQV
                     TPRADDRARIVVVDRPSSVQTELMIGAPGPDRRHPGGWAPYPLLGFLVGGSPTARIDA
                     VLREDKGYTYGIRSTFRPRRRGGVFITAGSVRSEVTGDALRLLLDVLEHGREGFTQDE
                     VDHAAQFLVLTAPSRYATADTIADEAASLSFDGLDTGFTSRNLVEIAGMDAERAGAAY
                     REFIDGRWTVVLVGDAASFLADVQALGRGDVTVVPN"
     gene            complement(7492..8793)
                     /locus_tag="PLK64_07355"
     CDS             complement(7492..8793)
                     /locus_tag="PLK64_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPV79598.1"
                     /translation="MADIAYPIAERTLPNGLRVIVSQDRTVPSVTVNLWVKVGSRDEV
                     PGRTGFAHLFEHLMFQGSANVASGEHFSALMGVGGRLNATTWFDRTNYFETVPKGALE
                     LALWLEADRQGRLLDALTQENLDNQRDVVKEEKRQRYDNAPYGHAMRDVYATLFPEGH
                     PYHHPTIGSMEDLEAATLDDVHAFFATHYGPNNTVLTLVGDISPEDGFAAVEKYFGPL
                     PPSAVARTEGSMPPLAPLTGPVRLERSEPVPNDRLYLAFRLPADATPEFHAVGLAMDA
                     IGGLSSSRLVEHLVRQERLVTGLTAHPLGFAGGVSLGLISADISAGVDPADVERAICA
                     ELERFAETGPTDIEMESAVAETERAWLSALASHDDRADHISHYAALHDDTAYINTFLA
                     QVRAVTVEEVRAAAARYLQPSSRAVVAYLMAPGPIADEELS"
     gene            complement(8863..9408)
                     /locus_tag="PLK64_07360"
     CDS             complement(8863..9408)
                     /locus_tag="PLK64_07360"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HPV79599.1"
                     /translation="MDPAPGDSRHDPAVGSAAGSAVGSTVGSAQGPRTFSWDFQVRFI
                     DLNASGHLDNLQILRAVDEARHRLLGVGEPGAAPLLVGLLADAPAHVATLVAAHRVEY
                     RRELWFAAAPLDITLWVCRVGGSSFDVATEVRQSPAEPIAAVAVTTVVVVDTTIGSPW
                     PLSAPVRAALTAYLGPAPTLR"
     gene            9441..>9869
                     /locus_tag="PLK64_07365"
     CDS             9441..>9869
                     /locus_tag="PLK64_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79600.1"
                     /translation="MTASPTGGGVRVLRATTLAVVTLALAATAHVLAGGDLPGVLALA
                     LAAGPLALGTVLLTRRRLSLAAVFAWLVPAEITLHVYFGAAAPHPLVHAPAGGHVHGG
                     SDAGMPVAGLSGLDPLAQPIGTTPLGHSSLAMLGAHALATV"
BASE COUNT         1412 a         3486 c         3564 g         1407 t
ORIGIN      
        1 ccgagccccg cgggatcgtg tgggtgccgg ggacggcata catccgggtc ggcgggagcg
       61 acacggcggc gagtacctcg gccggggtcc cgaaagggtc gcgctcgcct tgcacgaccg
      121 acaccgggag cccgccggcg agcgcgccct ccagctcctg agcgcggctc gattcgggtt
      181 ttcccggcgg gtgcagcggg aagctcaggc acagcaccgc gtcggcgccg acgctgaggg
      241 cggtccggca ggcgacccgc gcgcccgcgc ttcggccggc gaccagccag gggccgcgcg
      301 cggtcgcgag cgccccgccg cgggcccgca gagcagcggc gaccgacacc caggccgtgt
      361 cgaggatggg cggccgcgca gcgacccgcc cgcctgcgac gagccacggt tgttcgacga
      421 gcgcgaccgc ccaacccgcg ccgacggcgg cctcggtggt ggcgatgagg tcggtggtgg
      481 tgatgctcgg gccggccccg tgaccgagca ggagcacccc acgggagccg cgcgcccggc
      541 gcaggtgcac ccgcgccgga ccgacggagg tgggcacttc caggaccgtg ggcgccggag
      601 tcagtggcgg ctgggtcgat gtcatgagcc gatgatctcg ccggtcgtcg ggtcgacgac
      661 cccgcgcagc gcggccgccg ggagggcgtc gagaaggtgc gcgccgttgc ccgccgaccc
      721 gttgaccgcc gtcgagacgg ggtatgccgc gaacctcccg gcccgctcga agcgcagcat
      781 ccgctcgaca gcggccgggt cggtcagggc ggggtcgagc caggcggccc agttgtccgg
      841 ctccaggacg agcggttggc ggtcgtggat ccggtccagg ccgggctccg cggcggtcgt
      901 gatgatggtg aaggtcgtca gccaggcgag cgggtcgtcc cggtcgggga ccgcggggtc
      961 gcgccagaac tcatagagac cggcgaaagc gaggggcgcc gcatctcccc gatggatgaa
     1021 gaagggctgc ttgcggggct tgcctttggc gtcgagcacc gtcggcgacg cctgccactc
     1081 ataccaacca tctgcgggga cgaggcaccg ccgggccagc gcggcccggg cgaacgggcc
     1141 cgattcgagc gcggtctcgg cgcgggcgtt gatgaaccgc atcccgatct tgacgtcctt
     1201 tgcccaactg ggcaccaggc cccaggtgag cagtcgcagt tgccggctca agggaccttc
     1261 tgggtccgag cgcgacgccc gggtgaggac gaccggtgcc tgcttgctcg gcgcgacgtt
     1321 gtagtcgggg gcgccggccg gcggatcctg tggtttgacc aggacgctgc gcgagggctc
     1381 ggccgtggcg accacctcga cgtcgaactc cagcacgagg tcggcctggt ccttggcagc
     1441 ggcatacctc ccacacatga gggacagggt atgccgcgct accgacggca tgcagttgca
     1501 gcagcctcca gggtgcgacc aactacggtt atcgggtgag catcgatgtc gaccaacagt
     1561 tgtggcctgc gcccgtggcg cccgggccgt tggacgctgt cgtgcgcctg cccggcagca
     1621 agtcgctgac caaccgctat ctcgtgttgg ccgcgttggc cgacgggcct tcgcggttgc
     1681 gggccccgct gcgatctcgc gacacgctgc tcatggccgc ggccctcggc actctcggag
     1741 cagacatccg cgacaccggc gccctcaacg cagtggcacc cgaaagcagc ggtgagcggg
     1801 cggcataccc ggtgagcgac tgggtggtca ccccaggtgc ggtgcgcggg ggcgggcacc
     1861 tcgactgtgg actggccggc accgtcatgc gtttccttcc cccggtggcg gctctggctg
     1921 acgggccggt ggccttcgac ggcgacgcgg gtgcgcgacg acgaccgatg ggtccggtcc
     1981 tcgacgcctt gcgtgccctg ggggtcggtg tggacgacga cggctcgggc tgtctgccgt
     2041 tcaccgtcca ggggcggggg cgcgacgggg tgcccggcgg cgaggtcacc ctcgatgcct
     2101 cggcgagctc gcagttcgtg tcggcgctgt tgctggcggg cgcccgctac gaccgcgggg
     2161 tgaccatcca tcacgacggc aagccggtgc cgagccagcc gcacatcgag atgacggtgc
     2221 agacgctgcg cgaggccggg gtggtcgtcg acgactccga cgccaacacc tggcgggtgg
     2281 cgccggggtc gatccgcggt ctggatgtcg tcatcgagcc cgacctgtcc aatgccgggc
     2341 cgttccttgc cgccgccctc gtggccggcg ggacggtgct cgtgcccgac tggccggagc
     2401 gcacgacaca ggcgggcgac cttctgcgcg agatcctggc cgccatgggg gcgcgggtgc
     2461 agttgggctc cgacgggctg cgggtgacgg gcagcgggcc gatcgagggc atcgacatcg
     2521 acctgcacga agccagcgag ctcaccccca ccgtcgccgc gctcgccgca ctggctgggt
     2581 cgccatcgtg gatccgagga gtcgcccaca tccgcgggca cgagaccgat cggctggccg
     2641 ccctcgacac cgagttgagc gccctcggca cccaggtgac ccagaccgag gacgggttac
     2701 ggatcgttcc ggcaccgttg cgcggcaatc ggttcggcac ctaccacgac caccgtatgg
     2761 caactgccgg ggccattctc gggctgcgcg tcccgggtgt ccttgtcgag gatgtcgcca
     2821 cgacggccaa gacgctgccc gacttcaccg gacgttggac ggcgatgttg ggcgcgagtc
     2881 acggcggagc cgagggcaac gggtgagctg ggaccacctc gacgagtcgg acgttcgagt
     2941 ccgcccgggt cgcgggtcgc ggccacggac gaaggaccgt ccggcccacg gcgacgcctt
     3001 ggtgggcacc gtcgtcaccg tcgacagagg ccgctacggc gtgctcatgg cggccggggt
     3061 cggggggcgc aaggtccccg aacgcatggt cgtcgcgatg aaggcccgcg agttggggcg
     3121 caagggaatc gtcgtcggtg acgacgtcgg gttggtcggc gactcctccg gacaccccga
     3181 tgccctcgcc cggatcgtgc gcgtcgagcc gcgccgcacc gtgttgcggc gcagcgccga
     3241 cgacaccgac cccatcgaac gggtgctcgt cgccaacgcc gaccaactcg tcgtcgtctc
     3301 atccctcacc gacccgccac cgcggccccg gttgatcgac cgttgccttg tcgccgccta
     3361 cgacgccggg ctggagccgc tgctcgtcct cacgaaggcc gacctggtgc cggccgacga
     3421 cttcatcggg caatacgccc cgctggccgt gcgctgtgtc atcacctccc gccgacccga
     3481 tcgcaccctc atcggcctcg aagatgtgcg ggcccaactc gccggccggg tgtcggtgct
     3541 cgtcgggcat tcgggggtcg gcaagtccac cctcgtcaac gctctcgtcc ctggagccga
     3601 ccgcagcgtg ggccgggtca acgacgtcac ggggcgcgga cggcatacct ccacgtcggc
     3661 catcgccctg cgactgccga acgatccggg gtgggtgatc gacacgccag gggtgcgctc
     3721 gttcgggttg gcgcacgtcg acgtcgcccg gatcgtgtcg cacttccccg accttgagcc
     3781 gggcaccgag acctgcccgc gaggctgcac ccacgacgaa cccgagtgcg gcctggacga
     3841 attcgttgcc aacggcggcg cgggcgccag cggcccggct cgcctcgact cgttccgccg
     3901 actgctccgc gcccgcaccg gcgatgtcga ctccgacgcc tgaggacgcc gcggaccgcg
     3961 agacggcgag cgcccaggga tcgggactcg cggcccacgg ggtctccggc cacccgagca
     4021 caccaacggc gcgcttggtc ggggcgctgc ccgtcatcgt ggggttcatc gcgttggcca
     4081 tcggcgtgac ggtgacccaa gagacctacg agtcggttca gtccggacgc ggggccgccg
     4141 cgttcgacga gcccgtgcag acgtggatgg tcgcccatcg cacgccttgg ctggacacgg
     4201 tggcgacctg gttcacccac ctgggcggca aggtcgggat gacgatcctc gccaccgtca
     4261 cggtggtggc cctggcccgt cgctggcgca cctggtcacc ggtgctcatc atgctgtggg
     4321 cgacggcggg gtcgctgacg ttgacgaccc tcggcaagac gttgaccgcc cgcgcgcgtc
     4381 ccccggcggc gcaggcggtg ccaccgctcg agacatccgc gtcgttcccg agtgggcaca
     4441 cgctcaacgc cgtcctcgtg accagcatca tcggctacct cgtcctgctc catgtgcgca
     4501 gccggcgggt gcaggtgctc acctggtgcg ggttggccct gttcgcgacc ctcatcggcc
     4561 tgtcccggat ctacctcggc caccattggc tgaccgacgt catcgccggc gccgctgtcg
     4621 ggggcgcctg ggcggccgcc gtcatcctcg gccatcacct ctatctccgg ctgcgcgccg
     4681 acccctagcc gccacggtga gcgggcggca tacctctagc ctgtgaccgt gagttacgac
     4741 gacgatctcc gcctggccca cgtccttgcc gatgccgtcg agcgggtgac catggcccgg
     4801 ttccgcgccg aggacctgat ggtcgagacc aagccggacc tcaccccggt gagcgatgcc
     4861 gaccgcgcgg cggaggaact catccgccac caactggccc ggacccgccc gcgcgacgcc
     4921 gtggtgggcg aggagttcgg cacgaccggg catggcccgc gccgctggat cgtcgacccg
     4981 atcgacggga caaagaactt cgtccgcgga gtccccgtgt gggccacgct catcgccttg
     5041 gtcgacggcg acgagcccgt cgtcgggctg gtcgccgccc ccgctctcgc ccgccggtgg
     5101 tgggcggcca agggagccgg ggcgtatgcc ggtcgcagcc tgtccgccgc ccgtccgctg
     5161 cacgtgtcag gggtctctgc cctcgccgac gcgtcgctgt cctatgcctc attgggcggt
     5221 tggtcggaga tcgaccgacg cgaggacttc ctcgacctca tggacgggtg ctggcgcacc
     5281 cgcgcctatg gcgacttctg gtcctacatg ttggtggccg aaggggcggt tgacatcgct
     5341 gccgaaccgt cacttcagac ctatgacatg gctgccctcg tgccgatcgt caccgaggct
     5401 ggcggccgat tcaccgggct cgatggacgg cccggatgtt ggagcggcaa cgccgtcgcc
     5461 accaatgggc tgctgcacga gacggtgctg gacctcatcg gcgactgacg gccggggtca
     5521 gaacaactcg agctgatccc gttcggtcgc cacgccctcc aggtgcagga gggtctgctt
     5581 gcgagacagc ccaccggcat acccggtcag tgtgccgtcc gccccgatga cccggtggca
     5641 cggcagcacc acggggatcg ggttggcgcc gttagccgcg ccgaccgcgc gcgaggccga
     5701 cacgggcagc cccaaccgga gggccacctc gccgtaggtc acggtcgtgc cgtaggggat
     5761 caacgccaac tcgtgccaga cggcgcgctg gaaatcggtg ccgtgcggcg cgagcggcag
     5821 gtcgaacgag cgcagcgacc cggcgaagta ggccgccaac tgcctggccg cctcgacgag
     5881 caccgctgcc gtcggcgcct gcgctgacgt cggatcgctc gcctcgggtc gcccgtcgaa
     5941 ccggatggcg ctcacggcga ccccgtccac gctctcgagc cgcaacggcc cgatggggct
     6001 gtccacgacg agagccgagc gcatgtcgcg cagcctagtt gggcacgacc gtcacgtcgc
     6061 cccgtcccag ggcctgcacg tcggcgagga aactcgctgc gtcgccaacg aggacgaccg
     6121 tccaccgccc gtcgatgaac tcccggtatg ccgcgcccgc tcgctccgcg tccatcccgg
     6181 cgatctcgac gagattgcga ctggtgaacc cggtgtccaa cccgtcgaaa gacaggctgg
     6241 cggcttcgtc ggcgatcgtg tcggccgtcg cgtagcgact cggggcggtg agcacgagga
     6301 actgggcggc gtggtcgacc tcgtcctggg tgaagccctc gcggccatgt tcgagaacgt
     6361 cgagcagcag ccgcagggcg tcgccggtga cctccgaacg cacactcccg gcggtgatga
     6421 acacgccacc gcggcggcgc ggccggaagg tcgagcggat gccgtaggtg tagcccttgt
     6481 cctcacgcag gaccgcgtcg atccgagcgg tcggcgagcc gccgacgagg aatccgagca
     6541 acgggtaggg ggcccagccg ccgggatgcc gccggtccgg gccgggggca ccgatcatca
     6601 gctcggtctg caccgaactg ggccgatcga cgacgacgat ccgggcgcgg tcgtcggccc
     6661 tcggggtgac ctgccagggg ccggggctgc gataggtgtc gggggtcccc caccgaccca
     6721 ggctcaggtc caactcggcc accacgtcga gacccgccag gtcgccggcc acgacgaccg
     6781 tcgcctccgc gggtgcgacg tgctcggcgt ggaaggcgac gacggcgtcg cgggtgatag
     6841 ctgcgaccgt ctcggcggtg ccgccgaccg ggcgcgagga acgtcccact ggcgaataga
     6901 acgtggccgc gaactccgcc gccgcgcggt gcgaggcgac cgaacgctcc tgctcgatcg
     6961 cctcgcaccg cgcggcggcg gagttcgcgg ccacgcggtg cgaggcgacc gaacgctcct
     7021 gctcgatctc ggccagccgg gtcgagacct ggcgcgccac ctcctccgca gggaaggccg
     7081 gctcggccag gcactgatgc atgaggtcga gcccgtaggc caggtgatgc ttggcgacgt
     7141 cgagctcgac cgacaggccg gagtcgccga cgccgcaccc gatcgccacc ccgcgccgtt
     7201 cgagcaggcg cgcgaactcc acggaggtat gccgcgcggt cccctcatcg agggtccgcg
     7261 ccatgatcgt ggcgacgcct tcgaggtcgc gcggctcgac ccgcaacggc atgggcaccg
     7321 acagacgcac cgacaggacg tactgtccgg gcaggtcata ggccagcccc gacgcaccgt
     7381 tagccagccg gaaccgggtc ggcaccggga gctcgtaggg ctgcggcacc aggacgtcgg
     7441 ggcggggtag cgcgtccggg gcggacatcc gggtgcgctg cgggaccccg gtcatgacaa
     7501 ctcctcgtcg gcgatagggc ccggtgccat gaggtaggcg accacggccc gcgacgacgg
     7561 ctgcaggtag cgcgcagccg ctgcgcgcac ctcctcgacg gtcaccgctc gcacctgggc
     7621 aaggaacgtg ttgatgtatg ccgtgtcgtc atgcagcgcc gcgtagtggc tgatgtggtc
     7681 ggcccggtcg tcgtggctgg ccaacgccga aagccaggca cgctcggtct cggccaccgc
     7741 cgactccatc tcgatgtcgg tcggtccggt ctcggcgaag cgttcgagct cagcgcagat
     7801 ggcgcgctcg acgtcggcgg ggtcgacccc tgccgagatg tcggccgaaa tcaagccgag
     7861 agacacaccc cctgcgaagc ccagcggatg ggcggtgaga ccagtgacca accgctcctg
     7921 gcgcacgagg tgctcgacca gtcgagagga ggacagcccg ccgatggcat ccatggccag
     7981 gccgaccgcg tgaaactccg gcgtcgcgtc ggccggaagc cggaacgcga ggtagagccg
     8041 atcattgggc accggctcgg agcgctccag gcgcactggg cccgtcagcg gcgcgagggg
     8101 cggcatactc ccctctgtgc gggcgaccgc ggagggaggc agcggtccga agtacttctc
     8161 gacggcagcg aagccgtcct ccggagaaat gtcgccgacg agggtgagca ccgtgttgtt
     8221 cgggccgtaa tgcgtggcga agaaggcgtg gacgtcatcg agcgtcgcgg cttcgaggtc
     8281 ctccatcgac ccgatcgtcg ggtggtggta ggggtgccct tcggggaaga gcgtcgcgta
     8341 gacgtcgcgc atcgcatgac cgtagggcgc gttgtcgtag cgctggcgct tctcttcttt
     8401 gacgacgtcg cgctgattgt cgaggttctc ctgggtgagc gcgtcgagca gtcgaccctg
     8461 ccggtcggcc tcgagccaca gggcaagctc caacgccccc ttggggaccg tctcgaagta
     8521 gttcgtccga tcgaaccagg tcgtcgcgtt gagtcggccg ccgacgccca tcagcgccga
     8581 gaagtgctct cccgaggcga cattcgccga gccctgaaac atgaggtgtt cgaagaggtg
     8641 ggcgaaacca gtgcgccccg gcacctcgtc gcgcgacccg accttgaccc agaggttgac
     8701 ggtcacggat ggcaccgtgc ggtcctgcga aacgatgacg cgcaggccgt tgggcaacgt
     8761 ccgctcagcg atcgggtagg cgatgtcggc cacccgcaga ccttatcggg cgaaaggcac
     8821 ccccgtgttc cggttgccgg ggccccgccc aagcgggcgc cgtcagcgca gggtcggggc
     8881 cggccccagg tatgccgtga gcgcagccct gacgggagcc gacaggggcc agggcgatcc
     8941 gatggtcgta tcgaccacaa cgacggtcgt cacggcgacc gctgcgatcg gttccgccgg
     9001 agactggcga acctcggtgg cgacgtcgaa cgaggagccg ccgacacggc atacccagag
     9061 ggtgatgtcc agcggcgcag cggcgaacca gagctcccgg cggtattcca cgcggtgggc
     9121 tgcgacgagc gtcgcgacgt gcgccggcgc gtccgcgagc aggccgacga gcagcggtgc
     9181 ggcgccgggc tcccccacgc cgagcaggcg gtggcgggct tcgtcgacgg cccgcaggat
     9241 ctgcaggttg tccaggtgac cgctggcgtt gaggtcgatg aaccggacct ggaagtccca
     9301 ggagaaggtc cgcggaccct gggcagagcc gactgtcgag ccgactgcag agccggctgc
     9361 cgagccgacg gctgggtcgt ggcgggagtc accaggggcc gggtccatgc tcgaaaccct
     9421 aggcccttac cctgaaggcc atgacggcga gcccgacggg cggcggtgtg cgcgtgctgc
     9481 gtgcgaccac cctggccgtc gtcacgctgg ctctcgcagc gacggctcac gtgctggccg
     9541 gaggcgacct gcctggcgtc ctcgcactcg ccctcgctgc cggtcccttg gcgctcggaa
     9601 cggtcctgct gactcgacgc cggctctccc tcgccgcagt gttcgcctgg ctggttccgg
     9661 ccgagatcac cctccacgtc tacttcggag cggccgcgcc gcacccactg gtgcatgcgc
     9721 ctgctggcgg tcacgtccac ggcggctccg acgcaggtat gccggtcgcc ggactttccg
     9781 gcctcgatcc actcgcgcag ccgatcggga cgaccccgct cgggcactca agcctggcca
     9841 tgctcggcgc gcatgccctc gccaccgtg
//
LOCUS       DAOMZY010000102         9812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_6537_length_9812_cov_5.873424, whole genome shotgun sequence.
ACCESSION   DAOMZY010000102 DAOMZY010000000
VERSION     DAOMZY010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9812
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6537_length_9812_cov_5.873424"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="PLK64_07370"
     CDS             <1..174
                     /locus_tag="PLK64_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019523298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HPV79601.1"
                     /translation="VVTTAGSFDLLAEVVCEDDAHLLEVISQRIRTLPGVGATETFVY
                     LELNKQHYNWGTR"
     gene            171..1601
                     /locus_tag="PLK64_07375"
     CDS             171..1601
                     /locus_tag="PLK64_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPV79602.1"
                     /translation="MTIYPSEIWEPSSDFHTGTGKLYSEAARDHLWMHFTRHSVFEDR
                     DGAHTVPLIVKGDGAYIWDDRGNKYLDGLSGLFTVQVGHGRQELADAAMAQFQKIAYF
                     PLWSYAHPRAVELAERLAHYAPGDLNRVFFTTGGGEAVESAWKLAKQYYKLVGKPMKT
                     KVISRNIAYHGTPQGALSITGIPAAKAVFEPLVPGGHKVPNTNLYRAPEHLRDDPKAF
                     GLWAANRIAEAIEFEGPETVAAVFLEPVQNSGGCFPPPPGYFERVREICDEYDVLLVS
                     DETICAFGRIGDYFACNQLGYVPDIITTAKGVTSGYAPLGCMIVSDRLFEPFRHGLTY
                     FPHGYTFGGHPISCAVAMANLDIFEKEGLNAHVRETAPAFRASLETLLDLPIVGDVRG
                     EGFFYGIELVKDKATRETFDDGECERLLRGYLSKALFDAGIYCRADDRGDPVIQLAPP
                     LIIGQPEFDDITSRLRSVLQGAEAHL"
     gene            1598..2983
                     /locus_tag="PLK64_07380"
     CDS             1598..2983
                     /locus_tag="PLK64_07380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPV79603.1"
                     /translation="MTRPLWWEGGIPSPAAAPGVGPVGAGYAAQPPSEVDVAIVGGGF
                     TGLWTAYYLLGLDPSLSVVVLEAEHVGFGASGRNGGWVSALWPVSASTLARESGEAAA
                     RSMGAALRETVLEVGRVAADEGIDCDYRRGGVVLAARTPAQVERARGYAAAGAAWGDG
                     TVWLDLPEARDRVRMSGLLGATYNPNCARIHPRKLVDGLAAVVRSRGGLIVEGARVTS
                     ASPGRVVLESGTVVRTRHTVRATEAWTARLAGLRRRTTPVYSLMIATEPLPAGVWDEI
                     GLAQRETFADHRHVIIYGQRTADDRIAFGGRGAPYHWGSEIRPEFDSEPTVFAELEST
                     LRGLFPVLQGYAVTHRWGGPLGIARDWHPSVGYDPVARLGWAGGYVGDGVAASNLAGR
                     TLADLVLGRASGLTRLPWVGHVSPSWEPEPVRWLGVNAGLRVAGWADLEESLTGREAV
                     LGRVLSALTGH"
     gene            complement(3282..4202)
                     /locus_tag="PLK64_07385"
     CDS             complement(3282..4202)
                     /locus_tag="PLK64_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HPV79604.1"
                     /translation="MLGALQGFSTIAAVISVGFVLAHVGVLGMEARNILADLAFFVAS
                     PALLLVVLSKAPVSAVLSWGLVVAAGGALLVMAIAAAIAHWRWGMGLGETVLAALCSG
                     YVNSGNLGLPIALYVLGDITYAAPVLLLQLLVLTPVSMTVLDSQLPFAKPGVMSRVAG
                     LLRNPITVGALIGLVLSVTGRQLPSALLDPITLIGNLAVPCMLIAFGVALRLGQRPGK
                     DLGRVATLSGLKLVGQPVIAASLGAALGLRGHELFAVVVMAALPTAQNIFTYSLRYDR
                     GVYLTRDTIFVTTLASAPVILVAALVFGGA"
     gene            complement(4289..6190)
                     /locus_tag="PLK64_07390"
     CDS             complement(4289..6190)
                     /locus_tag="PLK64_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HPV79605.1"
                     /translation="MSTGAYSTAYAASLANPTAFWGEAAKDIDWITPPTTVLDDSRPP
                     FYRWFPDGTLNTCFNAIDRHVAGGRADQVAIHYDSPVTGRKDSFTYAELLAHVSRLAG
                     ALVGAGVGKGDRVLVYMPMVPEAVFAMLACARIGAIHSVVFGGFAPAEVAARIDDAKP
                     KLILSASCGIEPNRVVPYKPFLDEGIARSEHKPDQVVVLQREQARAELGERDVDWAEF
                     ISTPAADAGADCVEVAATDPLYILYTSGTTGKPKGIYRDNGGHAVALRWSMRNIYDVA
                     PGETMFTASDVGWVVGHSYIVYAPLLTGATTILYEGKPVGTPDAGAFWRIISEYGAVT
                     MFTAPTAFRAIKKEDPEATLLAGYDLSHFRQLFLAGERLDPDTYHWATDKLGKPVIDN
                     WWQTETGWPIAANCVGLERLPIKAGSPSVAVPGYDVQILDPTGEQLPAGTEGAIAIKL
                     PLPPGTFPTLWHDDERYVSSYLSAYPGYYLTGDGGFIDEDGYIYVMGRTDDVLNVAGH
                     RLSTGSIEAALAGHPAVAECAVIGVHDDFKGQIPRALVVVKSGVDVEADGDRIRKELV
                     DRVRSEVGAVASLRQVDIVAALPKTRSGKILRKTMREIADGHTPAVPATIEDAGVLDR
                     LAPVLRPHG"
     gene            6324..7451
                     /locus_tag="PLK64_07395"
     CDS             6324..7451
                     /locus_tag="PLK64_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HPV79606.1"
                     /translation="MEIDRLEQLRALLARGGALGGVRLQDLDLHAVEEQLLDRTDVTD
                     LVVLGGRLSPALTLHLIRHGAIVFPSDPKAPVEVYRARLYTADELYAGLSGPLGYAGT
                     PDARAFDWTMDRRSGRDAYVTMLRAIHDESMTDTLDEVLDGLPVVGVMGGHEAQRGSG
                     VYAAAARLGHLLAEGGCVVATGGGPGAMEAANLGAYAASPADLDAAIAALSKVPSYRP
                     DVGPWAELGLRVREHLGRRPDSGELRGLPLRSIGIPTWFYGHEPPNVFGQGIAKYFSN
                     ALREDGLLARCTAGTVVLPGAAGTVQEIFQGVTRLYYGRLADPTGTLPPLVLVGRSHW
                     QRLVPTWPLVTALAGNRPMAEVVHLVDTIEEAAAILLPSAD"
     gene            complement(7472..8671)
                     /locus_tag="PLK64_07400"
     CDS             complement(7472..8671)
                     /locus_tag="PLK64_07400"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HPV79607.1"
                     /translation="MYGSIKDELLATLAEIDAAGLYKRERQLTTAQSAHVGVGGGSPA
                     VLNFCANNYLGLADHPDVVAAAKAALDEWGFGMASVRFICGTQTQHTLLEDRLSAFLG
                     TEATILYSSCFDANGGVFEVLFGEGDAIISDELNHASIIDGVRLAKAARYRYRNADMA
                     DLRVQLEAARAAGARRVVIVTDGVFSMDGSYAPLPQICDLAEEYAAMVLVDDSHAVGF
                     VGEGGRGTPELFGVADRVDIVTGTLGKALGGASGGYVSSHREVVDLLRQRSRPYLFSN
                     SVAPAVVAGSLAALDLVASSGEARAALRANSTLFRELMTAAGFDLLPGSHPITPVMFP
                     GEDGARQATMIADHMLGNGVYVIAFSFPVVPKGKARIRVQLSAAHSEADVRACVAAFV
                     AARDAVG"
     gene            complement(8711..9244)
                     /locus_tag="PLK64_07405"
     CDS             complement(8711..9244)
                     /locus_tag="PLK64_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPV79608.1"
                     /translation="MTPSDDLMDAALAVVGEAGVPGLTWKRIDERAGAAEGATFSLYP
                     TMDLLVEAMLGRLITLDGQAWLATGTALNPTSAREFADRMAALTVSHIELMGQATRAR
                     FALFLGRPQAMAQGQAALLDVITVILDSIGVPDPAARAQHVADLVGGTALHALTVRQG
                     EPINQAELAAAFWRLLR"
     gene            complement(9319..>9812)
                     /locus_tag="PLK64_07410"
     CDS             complement(9319..>9812)
                     /locus_tag="PLK64_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPV79609.1"
                     /translation="QDTLGHRGRRGDPLFQARRTLHTGDALLTDRQRERLMVLFTPDE
                     HAQVEATWGVYQRIITAYREPDPREGKGLMQAVIDSLREGVPTVLTETRKLGRTLNQR
                     TDDVLAYFDRPGTSNGPTEAINGRLEHLRGSALGFRNLTNYIARCLFEAGGFRPRLHP
                     ALG"
BASE COUNT         1534 a         3293 c         3490 g         1495 t
ORIGIN      
        1 gtcgtgacga cggccggaag cttcgacctg ctcgccgagg tggtgtgcga ggacgacgcc
       61 cacctgctcg aggtcatctc gcagcggatc cggacgttgc cgggtgtggg agccacggag
      121 acgttcgtct atctcgaact caacaagcag cactacaact ggggcacacg atgacgatct
      181 acccgtccga gatctgggaa cccagctccg acttccacac cggcaccggg aagctctatt
      241 cggaggccgc ccgggatcac ctgtggatgc acttcacccg ccattcggtc ttcgaggatc
      301 gcgacggcgc ccacaccgtg ccgctcatcg tcaagggtga cggggcctac atctgggacg
      361 accgggggaa caagtacctc gacggactgt cgggcctgtt caccgtccag gtcggccacg
      421 gccggcagga gttggccgac gccgccatgg cacagttcca gaagatcgcc tacttcccgc
      481 tgtggtccta tgcccacccg cgggccgtcg agttggccga gcgccttgct cactacgccc
      541 ctggcgacct caaccgggtg ttcttcacga ccggcggtgg cgaggccgtc gagtcggcct
      601 ggaagctggc caagcagtac tacaagctcg tcggcaagcc gatgaagacc aaggtgatca
      661 gccgcaacat cgcttatcac ggcactcctc agggggcctt gtcgatcacc ggcattccag
      721 ccgccaaagc ggtcttcgag ccgctcgtgc ccggcggcca caaggtgccc aacaccaacc
      781 tctatcgcgc gccggaacac ctgcgcgatg acccgaaggc gttcgggctg tgggccgcga
      841 accggatcgc cgaggccatc gagttcgaag ggcccgagac cgtcgccgcc gtcttcctcg
      901 agccggtgca gaactccggg gggtgtttcc ctccgccccc cggctacttc gagcgggtcc
      961 gcgagatctg cgacgagtac gacgtgctgc tcgtctccga cgagacgatc tgcgccttcg
     1021 gtcggatcgg cgactacttc gcctgcaacc aactcggcta cgtcccagac atcatcacca
     1081 cggccaaggg cgtcacgtcc ggctatgcac cgctgggatg catgatcgtc agcgaccggc
     1141 tgttcgagcc gttccggcac gggctgacct acttcccgca tggctacacc ttcggtggcc
     1201 accccatctc gtgcgcggtc gccatggcca acctcgacat cttcgagaag gaagggctca
     1261 acgcccacgt ccgcgagacg gccccggcct tccgcgcctc cctggagacc ctgctcgacc
     1321 tgccgatcgt cggcgatgtc cgcggcgaag gcttcttcta cggcatcgag ttggtcaagg
     1381 acaaagccac ccgcgagacc ttcgacgacg gcgagtgcga gcggttgttg cgcggctacc
     1441 tgtccaaggc cctcttcgac gccggcatct actgtcgggc cgacgaccgg ggcgatcccg
     1501 tcatccaact cgctcccccg ctcatcatcg gccagcctga gttcgacgac atcacctctc
     1561 ggctgcgctc ggtcctgcag ggcgccgaag cgcacctgtg acccgcccgc tgtggtggga
     1621 gggcggcata ccctcccctg ccgcggcacc cggggtgggc cctgtgggcg cggggtatgc
     1681 cgcgcagccc ccgtccgagg tcgacgtcgc catcgtcggc ggcggcttca cgggcctgtg
     1741 gacggcgtac tacttgctcg ggctggaccc gtcgttgtcc gtcgtcgtgc tggaagccga
     1801 gcacgtcggg ttcggggcga gcgggcgcaa cggtggctgg gtgtcggcgt tgtggccggt
     1861 gtctgccagc acgttggcgc gcgaatccgg tgaggccgca gcgcgatcca tgggcgcggc
     1921 cctgcgtgag accgtcctcg aagtcggccg ggtcgccgcc gacgaaggca tcgactgcga
     1981 ctaccgacgt ggtggcgtcg tgctcgccgc gcggactccc gcgcaggtcg agcgagcccg
     2041 cgggtatgcc gctgccggcg cggcctgggg cgatggcacc gtgtggctcg acctccccga
     2101 ggcccgcgac cgggtgcgga tgtcggggtt gctgggggcg acctacaacc cgaactgcgc
     2161 gcgcatccac ccgcggaagc ttgtggacgg gcttgcggca gtggtgcgtt cgcgaggcgg
     2221 gctgattgtc gagggtgccc gggtcacgtc cgcgtcgccc ggtcgagtgg tcctggagtc
     2281 ggggacggtg gtgcggaccc ggcataccgt gcgggcgacc gaggcgtgga ccgcgcggct
     2341 ggcggggctg cgacggcgca cgaccccggt ctactccttg atgatcgcta ccgagccgct
     2401 gcccgccggt gtgtgggacg agatcgggct ggcccagcgc gagaccttcg ccgaccaccg
     2461 gcacgtcatc atctatggcc agcggacggc tgatgaccgg atcgccttcg gcggccgggg
     2521 ggcgccctat cactgggggt ccgagattcg gcccgagttc gattcggaac caacggtttt
     2581 cgcggagttg gagagcacct tgcgtgggct gttccccgtc ctgcaggggt atgccgtgac
     2641 ccaccgttgg ggcggaccgc tggggatcgc gcgagactgg catccgtcgg tgggttacga
     2701 cccggtggcg cggttggggt gggccggcgg ttatgtgggc gatggtgtgg cggcgagcaa
     2761 cctggccggg cgaacgctgg ccgatctggt gctggggcgg gcaagcgggc tgacccggct
     2821 gccgtgggtg ggccatgtct cgccgtcgtg ggagccggag ccggtgcggt ggctgggcgt
     2881 caacgccggg ctgcgagtgg cggggtgggc ggatctcgag gagtcactca cgggccgcga
     2941 ggcggtgctg ggacgggtgt tgtccgccct caccggccac tgagggggtc accctgacca
     3001 accctgacca accctgacca aaccggtcag cctgtcggcc catggcaccc cgttcgggtg
     3061 agcgaaaccg acgccagggc gccggtctcg ctcacctgag cggccgaatg gcgaagcccc
     3121 gcgctacaac accggcgagt gtggggaatt cgggcgcgac cggcataccc cttccccaga
     3181 tcaccggcga gtgtggggaa cttggggcag ccagggccgg gacctcccca gatcatcggc
     3241 gagtgtgggg aattcgggcg cgaccggcag cccagggtcg gtcaggcccc accgaagacc
     3301 agcgcggcca cgaggatcac cggcgccgag gccagggtcg tgacgaagat ggtgtcgcgg
     3361 gtgagataga cgccccggtc atagcgcagt gaataggtga agatgttctg cgcggtgggc
     3421 agcgcggcca tgacgacgac agcgaacaac tcgtgccccc gcaacccgag ggcggccccc
     3481 aacgacgcgg cgatcaccgg ctgcccgacg agtttgaggc ccgacaacgt tgccacccgg
     3541 ccgaggtcct tccccggccg ctgccccaac cgcagcgcaa ctccaaacgc gatcagcatg
     3601 cagggcaccg cgaggttgcc gatgagcgtg atcgggtcga ggagcgccga cggaagctgg
     3661 cggccggtca ccgacaggac cagaccgatc agtgccccca ccgtgatcgg gttgcgcaac
     3721 agcccggcca cccttgacat cacacccggc ttggcaaaag gcaattggga gtccaacacg
     3781 gtcatgctca ccggcgtcaa cacgagcagt tgcaggagca ggacgggcgc ggcatacgtg
     3841 atgtcgccga ggacatagag cgcgatcggc aggccgaggt tccccgagtt gacgtaaccc
     3901 gagcagagcg ccgccagcac ggtctcgccg agccccatcc cccagcgcca atgcgcgatc
     3961 gcggcggcga tggccatgac gagcagcgct ccgcctgcgg cgacgaccag cccccacgag
     4021 agcaccgccg acaccggagc cttggacagc accacgagca gcagggccgg cgaggcgacg
     4081 aagaacgcca ggtcggcgag gatgttgcgg gcttccatcc cgagcacccc gacgtgggca
     4141 aggacgaaac ccacggaaat gaccgcggca atcgtcgaga acccctggag cgcaccgagc
     4201 accgaagcat ggtgacaccg cgcgtgggag ggcggcatac ccgtccgtgg gttgacccca
     4261 cgggccggcc ggtatgccgc ccgcgcgcct atccgtgcgg gcgcagcacc ggggcgagcc
     4321 ggtcgagaac tccggcgtcc tcgatggtgg ccggcacggc tggcgtgtgg ccgtcggcga
     4381 tctcccgcat cgtcttgcgc aggatcttgc ctgatcgcgt cttgggaagg gcggcgacga
     4441 tgtcgacctg acgcagcgac gcgaccgcac cgacctctga gcggacccgg tcgacgagct
     4501 ccttgcggat ccggtcaccg tcggcctcga cgtcgacccc agacttgacg acgacgaggg
     4561 cccgcgggat ctggcccttg aagtcgtcgt gcacgccgat gaccgcgcac tcggcgacgg
     4621 ccgggtggcc ggccagcgcc gcctcgatcg agccggtcga caaccggtgt ccagcgacgt
     4681 tgaggacatc gtcggtgcgg cccatgacgt agatgtagcc gtcctcgtcg atgaagccac
     4741 cgtcaccggt gaggtagtag ccggggtagg ccgacaggta ggacgagacg taacgctcgt
     4801 cgtcgtgcca cagcgtcggg aaggtccctg ggggcagcgg gagcttgatc gcgatcgcgc
     4861 cctcggtgcc cgccggcaac tgctcgccgg tcgggtcgag gatctgcacg tcatagcccg
     4921 gcacggcgac cgacggcgac ccggccttga tcggcagtcg ctcgagcccc acgcagttgg
     4981 cggcgatcgg ccacccggtc tcggtctgcc accagttgtc gatgaccggc ttgccgagct
     5041 tgtcggtggc ccagtggtag gtgtcggggt cgaggcgctc ccccgcgagg aacaactgcc
     5101 ggaagtgcga caggtcgtag cccgcgagca gcgtcgcctc ggggtcttcc ttcttgatcg
     5161 cccggaaggc agtgggggcg gtgaacatcg tcaccgcgcc gtactccgag atgatccgcc
     5221 agaaggcgcc cgcgtcgggg gtcccgaccg gtttcccttc gtaaaggatc gtcgtcgcgc
     5281 ccgtgagcag tggcgcgtag acgatgtacg agtgcccgac gacccagccg acgtcgctgg
     5341 cggtgaacat cgtctcgccg ggcgcgacgt cgtagatgtt gcgcatcgac cagcgcaggg
     5401 cgacggcgtg cccgccgttg tcgcggtaga tgcccttggg cttcccggtg gtgcccgagg
     5461 tgtagaggat gtagagcggg tcggtagcgg cgacctcgac acagtcggct ccggcatccg
     5521 cggccggcgt cgagatgaac tcggcccagt cgacgtcccg ctcccccaac tcggcccgcg
     5581 cctgctcacg ctgcaggacg acgacctggt cgggcttgtg ctcggaccgg gcgatgccct
     5641 cgtcgaggaa gggcttgtag ggcacgaccc ggttgggctc gatgccgcac gatgccgaga
     5701 ggatgagttt gggcttggcg tcgtcgatgc gggcggcgac ctcggccggt gcgaacccgc
     5761 cgaagaccac cgagtggatc gccccgatcc gcgcacaggc cagcatcgcg aagaccgctt
     5821 cggggaccat cggcatgtag accaggaccc ggtcgccctt gccgacaccc gccccgacca
     5881 gcgcaccggc gagccgactc acgtgagcca gcaactcggc atacgtgaag gagtccttgc
     5941 ggcccgtcac cggtgagtcg tagtggatgg cgacctgatc cgcgcgcccg cccgcgacgt
     6001 gccggtcgat cgcgttgaag caggtgttga gcgtcccgtc ggggaaccac cggtagaagg
     6061 gcggccggct gtcgtccaga actgtcgtcg gtggcgtgat ccagtcgatg tccttcgccg
     6121 cctcgcccca gaaggcggtc gggttggcca ggcttgccgc gtatgcggtc gagtaagcgc
     6181 cagtgctcat gggtcatgag ggtgcccggt atgccgcccg cgcgccaccg gacgccggat
     6241 gcctaccccg tcctttgcgc ccggaacgcg cccacctgcg ccgaacggtc gtgcccgtcg
     6301 agcggctctg gcagagtgcc atcgtggaga tcgaccgcct cgagcagttg cgcgcgctgc
     6361 tggcgcgcgg cggggccctc ggtggtgtgc ggctgcagga cctcgatctg cacgccgtcg
     6421 aggagcagct gctcgaccgc acggatgtca ccgacctggt cgtgctcggc ggtcggctct
     6481 cccccgcgct caccctgcac ctcatcaggc acggcgccat cgtcttcccc agcgacccca
     6541 aggctcccgt cgaggtctac cgcgcgcgcc tctacaccgc cgacgagttg tatgccgggc
     6601 tgtccggtcc gctcggctac gccggaaccc ccgacgcgcg ggccttcgac tggacgatgg
     6661 atcgccgctc gggccgggac gcctacgtca ccatgctgcg ggcgatccac gacgagtcaa
     6721 tgaccgacac cctcgacgag gtgctcgacg ggctccccgt cgtcggggtg atgggcggcc
     6781 acgaggccca acgcggcagc ggcgtctacg cggcggcagc ccggttgggg cacctgctcg
     6841 ccgagggcgg ctgcgtggtc gccaccgggg gcggccccgg cgcgatggag gccgccaacc
     6901 tcggggcgta tgccgcgtcc cccgcggacc tcgacgccgc catcgccgcg ctgtcaaagg
     6961 tgccgtccta tcggcccgac gtcgggccct gggccgagct cgggttgcgg gtccgagagc
     7021 acttgggacg tcggcctgac agcggcgaac tgcgcgggtt gccgttgcgc agcatcggca
     7081 tcccgacctg gttctacggc cacgaaccgc ccaatgtgtt cggccagggc atcgcgaagt
     7141 acttctcgaa cgccctgcgc gaggacgggt tgctcgctcg atgcactgcg gggacagtcg
     7201 tcctgccagg ggccgccggg acggtgcagg agatcttcca aggggtcacc cggctgtatt
     7261 acggccggtt ggccgacccg acggggacgt tgccgccgct ggtgctcgtc ggtcgcagcc
     7321 actggcagcg cctcgtgccg acctggccgc tggtcaccgc cttggcaggc aatcgtccaa
     7381 tggccgaggt cgtgcatctc gtcgacacga tcgaggaagc cgcggcgatc ctgcttcctt
     7441 cagctgactg acgcagcgtc gggcggcgcc tttagcccac cgcgtcgcga gcagcaacga
     7501 aggcggcgac acaggcgcgc acgtcggcct ccgaatgagc ggccgagagt tggacccgga
     7561 tccgggcctt gcccttgggg acgacaggga aggagaacgc aatgacatag acgccgttgc
     7621 cgagcatgtg gtcggcgatc atcgtcgcct gccgggcccc gtcctcgcct gggaacatca
     7681 ccggcgtgat ggggtgcgag ccgggcagca ggtcgaagcc ggccgccgtc atcagctccc
     7741 ggaagagcgt gctgttggcg cgcagggccg ctcgggcctc accggaggat gccacgagat
     7801 cgagggcggc caacgagccg gcgacgacgg ccggggcgac cgagttggag aacaggtagg
     7861 gacgcgagcg ctgacgcagc aggtcgacca cttcccggtg gctcgagaca tagccaccag
     7921 aggcaccgcc gagggccttg cccaacgtgc cggtgacgat gtcgacccga tcggcgacgc
     7981 cgaagagctc cggcgtcccc cggccgccct ccccgacgaa cccgacggcg tgcgagtcgt
     8041 cgacgaggac catcgcggca tactcctccg cgaggtcgca gatctgcggg agtggcgcgt
     8101 aggagccgtc catcgagaag accccgtcgg tgacaatgac gacccggcga gccccagcgg
     8161 cacgcgcggc ctccaactgg acccggaggt cggccatgtc ggcgttgcgg tagcgatagc
     8221 gcgcggcctt ggccaggcgc accccgtcga tgatcgaggc gtggttgagc tcgtcggaga
     8281 tgatcgcgtc gccctcgccg aagagcacct cgaacacccc accgttggcg tcgaagcacg
     8341 aggagtagag gatcgtcgcc tcggtgccga ggaaggccga caggcgatct tcgagcaggg
     8401 tgtgctgggt ctgggtgccg cagatgaagc gcaccgatgc catcccgaaa ccccactcgt
     8461 cgagggccgc cttggctgcg gcgacgacgt cggggtgatc ggccaagccg aggtagttgt
     8521 tggcgcagaa gttgagcacg gccggcgacc caccaccgac gccgacgtga gccgactgcg
     8581 ccgtggtgag ctgacgctcg cgcttgtaga gccccgcagc gtcgatctcg gcgagagtgg
     8641 cgagaagttc gtccttgatg gacccgtaca cggaggtctc cttcgggtga gagcagtgag
     8701 cgcgtcgggg tcagcgcagc aaccgccaga aagccgcagc cagctcggcc tgattgatgg
     8761 gctcgccctg acgaacggtc aaggcgtgca acgcggtgcc accgacgagg tccgcgacgt
     8821 gttgggctcg cgcggcgggg tcggggacgc cgatggagtc aaggatgacg gtgatgacgt
     8881 ccagcagggc ggcctgaccc tgagccatcg cctgcggccg accgaggaac agggcgaacc
     8941 gcgcccgggt ggcctgcccc atcagctcga tgtgggacac ggtgagggcg gccatccggt
     9001 cggcgaactc gcgggccgag gtcgggttga gcgcggttcc ggtagcgagc caggcctgcc
     9061 cgtcgagggt gatcagtcgc cccaacatgg cctcgaccag caggtccatc gtcgggtaga
     9121 gcgagaaggt cgcgccctcc gcggccccgg cccgctcatc gatccgcttc caggtcagcc
     9181 ccggcactcc ggcctctccg acgaccgcca gagcggcgtc catgaggtcg tcggagggag
     9241 tcacggccgg aagcttattc cgtcggcgcg ggtcaagaac gcaggattgg ccagagaggt
     9301 tgccgaggat taactggctc atcccaaagc agggtgtagc cgtggtctga atccgccggc
     9361 ctcaaagagg cagcgggcga tgtagttggt gaggtttcgg aagccgaggg ctgagccgcg
     9421 taggtgctcc aggcggccgt tgatcgcttc tgttgggccg ttggatgtgc cgggtcggtc
     9481 gaagtaggcc aggacgtcgt cggtgcgctg gttgagagtc cggccgagtt tgcgagtctc
     9541 tgtcaggacg gtggggacgc cttcgcggag gctgtcgatc acggcctgca tcagcccttt
     9601 cccctcgcgt gggtcgggct cgcggtacgc ggtgatgatc cgctgataga caccccaggt
     9661 ggcttcgacc tgcgcgtgtt cgtcgggcgt gaacaacacc atgagtcgtt cccgttgtcg
     9721 gtcggtcaac aacgcgtcgc cggtatgcag tgtgcggcgg gcttggaaga gcgggtcgcc
     9781 acgtcggccg cggtggccga gggtgtcttg tt
//
LOCUS       DAOMZY010000103         9757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_6579_length_9757_cov_5.756545, whole genome shotgun sequence.
ACCESSION   DAOMZY010000103 DAOMZY010000000
VERSION     DAOMZY010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9757
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6579_length_9757_cov_5.756545"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PLK64_07415"
     CDS             complement(<1..438)
                     /locus_tag="PLK64_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HPV79610.1"
                     /translation="MAGRIRAEDILAVKERTSIEEVVREHVTLTRRGSALVGLCPFHD
                     EKTGSFNVNPHNGFYHCYGCGVGGDVIKFVSDIEHLTFTEAVERLAGKVGVELRYEDG
                     GGRREEAGGAGRRARLIEAHRVAQEFYAAYLLAAGDACAGRDFM"
     gene            complement(808..1863)
                     /locus_tag="PLK64_07420"
     CDS             complement(808..1863)
                     /locus_tag="PLK64_07420"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HPV79611.1"
                     /translation="MDSNAATLDSAECRVVLTAATSVATQLVAQLWQVQGAELGTVLG
                     MLDALAAVVCGARLAVVGEAEARGEISESQAGSTAGWVAEHAPTLAAGNGAGQVARLV
                     RECKRPDLTTVQAAALSGFVSVPVALTVVSEFARLKPRLREEAAPTVLQGLLDMGIAD
                     GPRGVRSLRPALLAAHGADGEFQADADTAERLISLSRPSTDELGALTYQLILDPVGAA
                     ALEAAIGPLSAPVPGPDGERDARSPQTRRGQALVEVCRRATSAGDRPPSGVKTTLMLT
                     MSYDDLAARIGAATVVGSAQSGSLIAPETVRRLACDAAIIPTVLGGRGEILDQGRSTR
                     LATPGQLAGTTPLRRPP"
     gene            complement(2066..3013)
                     /locus_tag="PLK64_07425"
     CDS             complement(2066..3013)
                     /locus_tag="PLK64_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPV79612.1"
                     /translation="MSEPVLALDIGGTKIAAALVTGGHVGEVCRVATPASQGPEAVVA
                     AAVAVAKEARGESRPVLVGIGSAGVIDSTNGVVTHATDALPGWAGTVLARGVSEGLGL
                     AAYALNDVHAHALGEASFGAGRGLRDVLLVAVGTGVGGAVVHRGEVIFGAHAVAGHLG
                     HIPASEAAGLPCTCGRVGHLEALASGPGLAAALRREGGSAQNGRDVVVQAGQLGAAGE
                     AARAVLTLGGVALGRVIGGLVNTLDPDVVVLAGSVADAGLIWWEAVQDGVRQEAMDAV
                     RGIPLVPAGAGAAAALLGAAHFAMTRHASKTVADDGQGL"
     gene            complement(3010..3927)
                     /locus_tag="PLK64_07430"
     CDS             complement(3010..3927)
                     /locus_tag="PLK64_07430"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016663387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPV79613.1"
                     /translation="MPRGLTGVVPPLVTPLTPEGELDVPSLERLVARLLDAGVDGIFA
                     LGSSGEVAFFEDRMRAKVLDAVTAVVAGAVPVIAGVIDMQTRRVLEHVRAAEAAGVQA
                     VVATAPFYAITGPDEIRAHFRAIGAATDLPVYAYDLPICVHTKLAAPMLVELGLEGRI
                     VGVKDSSGDDVGFRWLCELNADAGSPLTLLTGHETVVDGAYLAGADGSVPGLANVDPA
                     GYVRLHRAAMAGDWATVRVEQSRLSRLMGIARAPIGKVGPAAGVGSFKTALALLGVLS
                     TNTMSLPMASLEGADVAAVRAVLEEGGLL"
     gene            complement(3927..>4754)
                     /locus_tag="PLK64_07435"
     CDS             complement(3927..>4754)
                     /locus_tag="PLK64_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPV79614.1"
                     /translation="QTVPQDHATAGAPVIELRGVHVIHKARTGKLFRPDRVHAVKGVD
                     FTVARGETVGIVGESGCGKSTVAKVMVGLQQPTKGQVLFHGKPIGTSASARKELGRAV
                     SVVFQDPATALNPRMTVRDQLLDPLGVHGIGTPATRLARVRELVHLVGLPQSALEVLP
                     RQISGGQRQRVAIARALALEPDVIVADEPTSALDVSVRAQVLNLLTDLKEALGLGLVF
                     ISHDINTVRYVSDRIAVMYFGEIVETGPAEQIFTSPRNDYTRTLLSATPSLLEIGAS"
     assembly_gap    4755..4854
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4855..6824)
                     /locus_tag="PLK64_07440"
     CDS             complement(<4855..6824)
                     /locus_tag="PLK64_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HPV79615.1"
                     /translation="MLRPTLTKSLESRPGFRLPAIQSLPITSKIALGVIALVAAMAVF
                     APLLGVDPLASGTPVLPPGPGHPFGTDAIGRDILARVAFGARSSLVLGLVATGVALAA
                     AAVLGSIAATAGKATSEVLMRILDIVMSFPGIALAAVFVAVFGNALPVLIFAIAFLYT
                     PQLTRVVRANVLAQFGEDYVSASRVMGARPAHILIKHVARNCLAPIMVFATVLVADAI
                     VFEASLSFINAGVKPPNPSWGNILADGKQLLLSGYWWPTFFPGLMILITVLSLNILSE
                     GLTDALASPAVKRRVDVEADEKALLQGTAAAPVGAQVLGQVDGAVAKQLLTTSLVTLR
                     EQELKRTDRLVYAADSAPLLEVRDLSIRFPQAHGEVAIVDRVSFDVRPGETMALVGES
                     GCGKSITALAIMGLLSPAAQIEGEVFFDGRDLLTMDRKERIGLLGHDLAMIYQDALSS
                     LNPSMLVKSQLAQLTKRGGKRSAEDLLELVGLDPARTLKSYPHELSGGQRQRVLIAMA
                     LTRNPRLLIADEPTTALDVTVQQQVVGLLNTLREQLGFAMVFVSHDLALVAKLAHRIT
                     VMYAGQVVEQAPTSELLSNPRHEYTRGLLGSVLSIEAGAARLHQVPGTVPSPREFLPG
                     DRFAPRSSYPDLGRDERPTLRPAGPDHVYAST"
     gene            complement(6824..7789)
                     /locus_tag="PLK64_07445"
     CDS             complement(6824..7789)
                     /locus_tag="PLK64_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012577046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV79616.1"
                     /translation="MSNLLRLIGRRLVAFPLMVLGVTLLVFVVMSFSPSDPARLALGE
                     AASPDALAQYRTDHGLDDPLLVRYGSFLVGMLHGDLGTTSGNTPVTSVIAGAFPITLQ
                     LTLLGLVIAVVLALILGVVAALYRDRWPDQVIRVVSIMALATPSFWLAILLIQWLGTV
                     PGGWGMFPALVLGWVPFGEDPATYLNNVALPAFALGVPVAGSLTRVVRTAMVEELDRD
                     YVRTALGSGIPRREVIARNVLRNALITPITVLGLRVGYLMGGAVIIEIIFNIQAMGQL
                     ILDGVTRNDVYLVQGVTLTVAIAFIVVNILVDVLYVLVNPRIRSI"
     gene            complement(7928..9541)
                     /locus_tag="PLK64_07450"
     CDS             complement(7928..9541)
                     /locus_tag="PLK64_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPV79617.1"
                     /translation="MQVLDQQSSRRGFLQLAGAMGAAAAFAGTVSACAPGSSTTTGAS
                     GAAGGAKADGTITAAISYELGTNGYDPMTTSSALTIAANWHVMEGLTEVDPASREVYA
                     ALAKELPKATGTSVDIALRDGAVFHNGSAVTADDVVFSIERVLDPANKSLYRQFIPFI
                     KGVAKKDEKTVTLTLAYPSALLAARLAVVKIVPKAVVTADAKAFDAKPIGTGPWKLTD
                     NGATSKQVTFERNDAYTGSKKARAKAMKWQIIPDASTRTNAVQSKSVQAIDSVPYLSI
                     DQLKTTSQVDSVQGFGLLFAMFNNSAGNPFATKENRQAFFYAIDMAKVVQTAMLGQAS
                     PATSFVQEGHPQYVKAKTVYSHDAAKATAMFKATGLTKLRLLCTDHDWVKKCTPLIQE
                     SLKAAGLEVEFTEKKSADVYNTIDGKPEAFDVVIAPGDPSVFGNDLDLLMRWWYAGDV
                     WTDTRMHWKGSAAYTQVQSLLDAGLQASDEAKAKESWASLIDLIADEIPLYPLFHRKT
                     PTAYDKASLVDFKPIPLTGLSFVGVASTK"
BASE COUNT         1615 a         3299 c         3339 g         1404 t
ORIGIN      
        1 catgaagtcg cgccccgcgc aagcatcccc cgccgccagc aggtatgccg cgtagaactc
       61 ctgggcgacc cggtgcgcct cgataagccg ggcccggcga ccggcacccc cggcctcctc
      121 gcgccgcccc ccgccgtcct cgtaacgaag ctcgacgccg accttgccgg ccaagcgttc
      181 gaccgcctcg gtgaaggtga ggtgctcgat gtcggagaca aacttgatga cgtcaccgcc
      241 gaccccgcag ccgtagcagt ggtagaagcc gttgtgcggg ttgacgttga acgacccggt
      301 tttctcgtca tggaacgggc acagaccgac cagcgcggag ccgcgtcggg tgagggtgac
      361 gtgctcgcgg acgacctctt cgatcgaggt gcgctccttg acggcaagga tgtcctccgc
      421 ccggatcctt cccgccaccc acgccacctc ctcgcttgct gccgcgagtg tatgccgtcc
      481 cgccgacggt ccgcccgccc ctaggtgtcg tcacctggtc gcgagtcaac ccataggtct
      541 gtccacaggt ccgaggtgga ggcaggggtg tcccacttgt cgtaatcggg tgatcgacgc
      601 tgccggtgtg cgggcgggtc ccagggttgg aggcctcccg ggagttggtc gcccggggat
      661 tcgtcacccg ggttctgatc gcccggggat cggccgccgc gcggtggggg tccgtcgtcg
      721 cgacggtggt aactgccggg gatgaggtcc caggtgacag tgcccgtgtc agcgccagtg
      781 tcggcggtaa cgcgcccgat gagttggtca cggtggacga cggagtggtg tcgtccccgc
      841 gagttgcccc ggcgtcgcca gccgcgtcga gcggccttgg tcgaggatct cgccgcggcc
      901 acccaggacg gtcgggatga tcgccgcgtc acacgccaag cgacgcaccg tctccggcgc
      961 gatcagggaa ccactctgcg cggatcccac cacggtggca gccccgatcc tcgccgccag
     1021 atcgtcgtaa gacatcgtca gcatcagcgt ggtcttcacc ccggacggtg gccggtcgcc
     1081 ggcgctggtc gctcggcggc atacctccac caatgcctga ccgcgacggg tctgggggct
     1141 acgagcgtcg cgttccccgt cagggccagg gaccggcgcc gacagtggcc cgatcgcggc
     1201 ttcgagagct gcggccccga cggggtcgag gatcaactga taggtgaggg cacccagctc
     1261 gtcggtggat ggtcgcgaga gcgagatcaa ccgttcggcg gtgtcggcgt cggcctggaa
     1321 ctcaccgtcg gcaccatgcg cggccagtag tgccggccgt agtgatcgga ccccgcgtgg
     1381 gccgtcggcg atccccatat ccagcaatcc ctgcaggacg gtcggggcgg cttcttcacg
     1441 caggcgcggc ttcaaccggg cgaactcgct gacgaccgtc aacgcgacag ggacactcac
     1501 gaaaccagag agggcggcgg cctggacggt ggtcaggtcc gggcgcttgc attcgcggac
     1561 gaggcgggcg acctgaccgg caccattccc agccgccaac gtcggtgcgt gttcggcgac
     1621 ccagccagcc gtcgaccccg cctgagactc ggaaatctcc ccacgagcct ccgcctcacc
     1681 aacgaccgcg agcctggcac cgcaaacaac cgcagccagc gcgtcgagca tgcccaacac
     1741 ggtccccaac tccgcaccct ggacctgcca caactgcgcc accaactgag tagccacgga
     1801 cgtcgcagca gtcagcacaa cccggcattc agccgagtcg agagtcgccg cgttgctgtc
     1861 cataaccccg acgctaacag cgaccactga cagaccccct accccaaaag gcctctccca
     1921 caaccgaaca catcacaacc aggtaacgtt ccgggccgcc gctagggcaa tgtcggccta
     1981 gacacgcgcg ggacaccacc ggcgacagat ccagaggcct caggcctcca ggacgacacc
     2041 tcccaaacct cacctgtcac tgtcgtcaca ggccctggcc gtcgtcggca acggtcttcg
     2101 acgcgtgccg ggtcatcgcg aagtgggccg ctccgagcag cgccgcggcg gccccggcgc
     2161 ccgccggcac gaggggtatg ccgcgcaccg cgtccatcgc ctcctgccgg accccgtcct
     2221 gcaccgcttc ccaccagatc agtccggcgt cggccaccga accggcgagc accaccacat
     2281 ccggatcgag ggtgttgacc agaccgccga tcacccggcc gagggccact ccgcccaggg
     2341 tgaggaccgc tctggccgct tcgcccgccg cacccaactg cccggcctgg acgacgacgt
     2401 cccgcccgtt ctgcgccgac cctccctcgc gtcgcagcgc cgcggccagc cccgggccgc
     2461 tggccaacgc ctccaggtgc cccactcgcc cacaagtgca cggcagtccc gccgcctcgc
     2521 tcgcggggat gtgaccgagg tgaccggcca ccgcgtgagc gccgaaaatg acctccccgc
     2581 ggtgaacgac cgcgccgccc acacccgtgc ccacggccac gagcagcacg tcgcgcaggc
     2641 ccctcccggc cccgaacgaa gcctcaccca acgcgtgcgc atggacgtcg ttgagcgcgt
     2701 atgccgcgag ccccaggccc tcgctcacgc cgcgggccag tacggtcccc gcccagcccg
     2761 gcagcgcatc cgtggcatgg gtgacaacac cattcgtgga gtcgatcacc cccgccgagc
     2821 cgatcccaac cagcaccgga cggctctcgc cgcgcgcttc cttcgccacg gcgaccgcag
     2881 cggcgaccac cgcctcaggc ccctgcgagg ccggggtggc gacccggcat acctcgccga
     2941 cgtggccgcc cgtgacgaga gccgcggcga tcttcgtgcc gccgatgtcg agggcgagca
     3001 cgggctcgct cacaagagcc cgccctcctc caggaccgca cgcacggcgg ccacgtcggc
     3061 cccctccaga ctggccatgg gcagcgacat ggtgttggtc gacaggacgc cgagcaacgc
     3121 cagcgcggtc ttgaacgacc cgacccccgc ggccgggccg accttgccga tcggtgcgcg
     3181 ggcgatcccc atgagtcgac tcagccggga ctgctcgacc cgcaccgtcg cccagtcgcc
     3241 tgccatcgcc gctcgatgca gccggacgta accggccgga tccacgttgg ccagaccggg
     3301 gacggatccg tcggcgccgg cgagataggc gccgtcgacc acggtctcgt ggccggtcag
     3361 cagggtgagc ggtgagcccg cgtcggcgtt gagctcacac agccaacgga agccgacgtc
     3421 gtcgcccgag gagtccttga cgcccacgat ccgcccctcc aggccgagct cgacgagcat
     3481 cggcgcagcg agtttggtgt ggacgcagat cggcaggtcg taggcataga ccggcaggtc
     3541 ggtggccgcc ccgatcgccc ggaagtgggc cctgatctcg tcggggccgg tgatggcgta
     3601 gaagggcgcg gtagcgacca cggcctgcac cccggccgct tcggcggccc gcacgtgctc
     3661 cagcacccgg cgggtctgca tgtcgatgac cccggcgatg acggggaccg cgcctgccac
     3721 gacggcggtc actgcgtcca ggaccttggc ccgcatccga tcctcgaaga aggccacctc
     3781 gccggaggac ccgagggcga agatcccatc gaccccggcg tccaggagcc gagcgaccaa
     3841 tcgctccagc gagggcacat ccaactcacc ctcgggggtg agtggagtga ccaggggcgg
     3901 cacaacgccg gtgaggccgc ggggcatcac gaagctccga tctcgagcaa ggagggggta
     3961 gcggacaaga gggtgcgggt gtagtcgttg cggggcgacg tgaagatctg ctcggccgga
     4021 ccggtctcga cgatctcccc gaaatacatg acggcgatcc ggtcagagac gtagcgcacg
     4081 gtgttgatgt cgtgcgagat gaagaccaag ccgaggccga gggcttcttt gaggtcggtg
     4141 agcaggttga gcacctgcgc ccggaccgac acgtccaacg ccgaggtcgg ctcgtcggcc
     4201 acgatgacgt ctggctcgag cgccagggcc cgagcgatcg cgacccgttg gcgctggcca
     4261 ccggagatct gccggggcag gacctccaag gccgactggg gcaggccgac gaggtgcaca
     4321 agctcacgga cccgagccaa ccgagtcgcg ggggtgccga tgccgtgcac gcccaacgga
     4381 tcgagcagtt ggtcgcgcac ggtcatccgg gggttgagcg ccgtcgccgg gtcctggaac
     4441 accaccgaca ccgctcgacc aagctccttg cgggccgagg cggacgtccc gatcggcttg
     4501 ccgtggaaga gcacctggcc cttcgtgggc tgctgcagac ccaccatcac cttggcgacc
     4561 gtggacttcc cgcagcccga ctcccccacg atgccgacgg tctcgccgcg ggccaccgtg
     4621 aagtcgacac ccttgaccgc gtggacgcgg tcgggccgga agagcttgcc ggtgcgcgcc
     4681 ttgtggatga cgtggacgcc acgaagctcg atgacgggcg ctcccgctgt ggcgtgatcc
     4741 tgcgggaccg tctgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcggtc
     4861 gaggcgtaga cgtggtcagg gccggccgga cgcaaggtcg gccgctcgtc cctgcccaga
     4921 tcggggtagg acgaacgcgg cgcgaaccgg tcgccgggca ggaactcccg aggcgagggg
     4981 accgtgccgg gcacctgatg cagccgcgcg gctcctgcct cgatggacag gaccgacccg
     5041 agcagacccc gggtgtattc gtgccggggg ttgctcaaca actcgctcgt cggtgcctgc
     5101 tcgacgacct gaccggcata catgacggtg atccggtggg cgagtttggc gacgagcgcg
     5161 aggtcgtggc tgacgaagac catggcgaaa ccgagttgct cgcgcagggt gttgagtagc
     5221 ccgacgacct gctgctggac ggtgacgtcc agcgccgtcg tcggctcgtc ggcgatgagc
     5281 agccgcgggt tacgggtgag cgccatcgcg atgagcaccc gctggcgctg accgccggag
     5341 agctcgtgcg gataggactt caacgtgcgg gccgggtcga ggccgaccaa ctcgagcagg
     5401 tcctcggcgc tgcgcttgcc gccgcgcttg gtcaattggg ccagttgcga cttgacgagc
     5461 atcgacgggt tcagcgagct gagcgcgtcc tgatagatca tcgccaggtc gtggccgagc
     5521 aggccgatgc gctccttgcg atccatggtc agcaggtcgc ggccgtcgaa gaagacctcg
     5581 ccctcgatct gcgccgcggg cgacagcaga cccatgatcg ccagtgcggt gatcgacttg
     5641 ccgcaccccg actcgccgac cagcgccatc gtctcgccag gacgcacgtc gaaggacacc
     5701 cgatcgacga tcgcgacctc cccatgggcc tgcgggaaac ggatcgacag atcacgcacc
     5761 tcgagcaacg gcgccgagtc ggcggcatac accagtcggt cggtgcgctt gagctcctgc
     5821 tcgcgcaagg tgaccagact cgttgtcagc agttgcttgg ccaccgcgcc gtcgacctgg
     5881 ccgaggacct gagcaccgac cggagcagcg gcggtgccct gcagcagggc cttctcgtcg
     5941 gcctcgacgt cgacgcggcg cttgacggcc gggctggcca gcgcatcggt cagaccctcc
     6001 gagaggatgt tgaggctcaa cacggtgatg aggatcatca ggccggggaa gaacgtcggc
     6061 caccaatagc ccgacaggag tagctgtttg ccgtcggcga ggatgttgcc ccagctgggg
     6121 ttcgggggct tgacgcccgc gttgatgaac gacagcgacg cctcgaagac gatcgcgtcg
     6181 gcgacgagca ccgtcgcgaa caccatgatc ggcgccaggc agttgcgggc gacgtgcttg
     6241 atgaggatgt gcgccgggcg ggcacccatg acccgactcg ccgacacgta gtcctcaccg
     6301 aactgagcga gcacgttggc ccggacgacg cgggtgagct gcggcgtgta gaggaatgcg
     6361 atcgcgaaga tcagcaccgg cagggcgttg ccgaacacgg caacgaagac ggccgccagc
     6421 gcgatcccgg ggaagctcat gacgatgtcg aggatccgca tgaggacctc tgaggtcgcc
     6481 ttgccggccg tggccgcgat cgagccgagc accgcagcgg ccgcgagggc gacaccggtg
     6541 gccaccaggc cgaggaccag cgacgagcgg gcgccgaagg ccacccgggc caggatgtcg
     6601 cggccgatgg cgtcggtgcc gaacggatga cccgggccgg gagggagcac cggcgtcccg
     6661 ctggccagcg ggtcgacacc gagcagcggc gcgaacaccg ccatggccgc gacgagggcg
     6721 atgactccga gggcgatctt ggacgtgatc ggcaacgact ggatggccgg tagccggaaa
     6781 ccaggccggc tctccagcga cttggtcagg gtcgggcgca gcatcagatg ctcctgatcc
     6841 gagggttgac caggacgtag agcacgtcga cgaggatgtt gaccacgatg aacgcgatcg
     6901 cgaccgtcag cgtgacgccc tgcacgaggt agacgtcgtt gcgggtcacc ccgtcgagga
     6961 tgagttggcc catcgcctgg atgttgaaga tgatttcgat gatgaccgcg ccgcccatga
     7021 ggtagccgac ccgcaggccg agcacggtga tcggcgtgat gagcgcgttg cgcaggacgt
     7081 tgcgggcgat gacctcacgc cgtgggatcc ccgacccgag agcggtgcgc acgtagtcgc
     7141 ggtcgagttc ctcgaccatg gcggtccgca cgacccgggt gagggagccg gcaacgggca
     7201 caccgagggc gaacgccggc agcgccacgt tgttgagata ggtcgccgga tcctcgccga
     7261 acggcaccca gcccaacacc agtgcgggga acatccccca cccaccgggc acagtgccca
     7321 gccattggat gagcaggatc gccagccaga aggacggggt cgccagcgcc atgatcgaca
     7381 cgacgcggat cacctggtcg ggccagcggt cgcgatagag cgccgccacc acgccgagga
     7441 tcaaggcgag cacgacggcg atgaccagcc cgagcagggt gagttgcagc gtgatgggga
     7501 aggcgccggc gatgacactc gtcaccgggg tgtttccgct ggtcgttccc agatccccgt
     7561 gcagcatgcc gaccaagaag ctgccgtagc ggacgagcag cgggtcgtcc agcccatggt
     7621 ccgtgcggta ctgcgccagg gcatccgggc tggccgcctc tcccagcgcg agccgggcgg
     7681 ggtccgaggg cgagaaggac atgacgacga acaccagcaa cgtcacgccg agcaccatga
     7741 gtgggaaagc gaccagtctg cggccgatga ggcgcagcag gttcgacaca ggagactcct
     7801 cgttcgcgtg gggcggtcaa cggtccgcca cggggatggt gcgcaggggc cggtatgccg
     7861 cgcacgcggc ataccgggtc cccgcaccta gcaagggggc acaacggggg cacagtcccc
     7921 cgacggccta cttcgtgctc gccacaccga cgaacgacag gccggtgagc gggatcggct
     7981 tgaagtcgac caggctcgcc ttgtcgtagg cggtcggagt cttgcggtgg aagagcgggt
     8041 agagcgggat ctcgtcggcg atgaggtcga tgagcgaggc ccaactctcc ttggccttgg
     8101 cctcgtcgct ggcctgcagc ccggcgtcca ggagcgactg gacctgggtg tatgccgccg
     8161 agcccttcca gtgcatccgg gtgtcggtcc acacgtcgcc ggcgtaccac cagcgcatga
     8221 gcaggtcgag gtcgttgccg aacaccgagg ggtcaccggg agcgatgacg acgtcgaagg
     8281 cctccggctt gccgtcgatg gtgttgtaga cgtcggcgga cttcttctcg gtgaactcca
     8341 cctcgagccc ggcggccttg agcgactcct ggatgagcgg cgtgcacttc ttcacccagt
     8401 cgtggtcggt gcacagcaga cgcaacttgg tcaggccggt cgccttgaac atcgccgtcg
     8461 ccttcgcggc gtcgtggctg tagacagtct tggccttgac gtactgcggg tggccttcct
     8521 gcacgaagct cgtcgccggt gacgcctggc cgagcatggc ggtctggacg accttggcca
     8581 tgtcgatggc gtagaagaac gcctgccggt tctccttggt ggcgaagggg ttgcccgccg
     8641 agttgttgaa catcgcgaac aacagcccga agccctggac cgagtcgacc tgcgaggtgg
     8701 tcttgagctg gtcgatcgag aggtaaggca ccgagtcgat ggcctggacg ctcttggact
     8761 ggaccgcgtt ggtccgcgtg gaggcgtccg ggatgatctg ccacttcatc gccttggccc
     8821 gggccttctt ggagccggtg taggcgtcgt tgcgctcgaa ggtgacctgc ttgctcgtcg
     8881 cgccgttgtc cgtgagcttc cacgggccgg tgccgatcgg cttggcgtcg aaggccttgg
     8941 cgtcggcggt gacgacggcc ttcgggacga tcttgacgac ggcaagccgg gcagccagca
     9001 gcgccgacgg gtaggccagc gtcagcgtga ccgtcttctc atccttcttc gccacgccct
     9061 tgatgaaggg gatgaactgg cggtagagcg acttgttggc cgggtcgagg acacgctcga
     9121 tggagaacac gacgtcgtcg gcggtcacgg ccgagccgtt gtggaacacg gcgccgtccc
     9181 gcagcgcgat gtccaccgac gtcccggtgg ccttgggtag ttccttggcg agggcggcat
     9241 acacctcgcg gctggcgggg tcgacctcgg tgaggccttc catgacgtgc cagtttgctg
     9301 cgatggtgag agccgacgag gtggtcatcg ggtcgtaccc gttggtgccc agctcgtagg
     9361 agatcgcggc ggtgatcgtt ccgtccgcct tcgcgccacc ggctgcgccc gacgcgccgg
     9421 tcgtggtgga ggatcccggt gcgcacgcgc tgacggtgcc ggcgaaggcg gctgcggcac
     9481 ccatcgctcc ggcaagctgg aggaaccctc ggcggctgga ctgttggtca agaacctgca
     9541 tggtgacggc gcctttctga ggaccttacg tgggcggcag tgcccttcat ccgacatcgg
     9601 acgtctgatg tccgatgatg atagtctgac gataggcccg gaagtgccgg ggagtcaaga
     9661 actgaagcag cggaggacga cacgccgatg agcgcaccgc gcaaggcccc tgcctggtcg
     9721 acgaccgatg cgatcaagca gcacatcatc gactcgg
//
LOCUS       DAOMZY010000104         9752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_6582_length_9752_cov_5.960709, whole genome shotgun sequence.
ACCESSION   DAOMZY010000104 DAOMZY010000000
VERSION     DAOMZY010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9752
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6582_length_9752_cov_5.960709"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PLK64_07455"
     CDS             complement(<1..337)
                     /locus_tag="PLK64_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S32"
                     /protein_id="HPV79618.1"
                     /translation="MDAQQETRALIRPVKESIEDDLLAREGVVGVDIAEKVSDGKPTG
                     QLSIVVFVQKKKPGARIARGQLIPKEIDGVPTDVQELEIELQSAMVKLDDVVGLVDAT
                     AYPSLHGGIS"
     gene            556..2181
                     /locus_tag="PLK64_07460"
     CDS             556..2181
                     /locus_tag="PLK64_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPV79619.1"
                     /translation="MSAIQPPAQPPAATSLPAPSAIPPTPQLSPEVAQRYGARILDPV
                     TGMRRSDGSAPAATVYVGERLLMRTTPGRALDDTVQALNEVTKDFGVRAERIPTPPAK
                     GERALHPEAMAVLQRHWVDGVRLLPVDDQVRMPVDAWPVLAAVASRDAAAAAPLALDH
                     LMTTAPSIGGTPYWLGTPYWLGTPYWLGTPYWLGTGSAAGEYGRPGSGGRTPVSVVLP
                     DPALTAPPVGRRPVVAVPDTGLGNHPWFPAGGAATSEVRWGGILLGINGPAGGGYPPA
                     PQNPLDPVIESDTGHGTFIAGLIRQLCPQAEVLAIPVMRGDGVVPESVLLNTLTLLLA
                     RHVEALATGSTDGLVDVLSLSLGYYHESPADAAMDAPLGALLRDFGDHGVAVIAAAGN
                     SSTLAPMFPAGLSADVTGFSPDSVPLASVGALNPDGETVAFYSNDGDWVSTFRTGSSL
                     VSTFPVDVTASAQASARVDFAGRARTTQDPDDYRGGFAAWSGTSFAGPVLAGEVAAAL
                     ARDADLATVDKAAAVARGRRAMFGQVTEWKGKE"
     gene            2178..2768
                     /locus_tag="PLK64_07465"
     CDS             2178..2768
                     /locus_tag="PLK64_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPV79620.1"
                     /translation="MTPSAPPTLADRAAAAFAAYREGDRERMGELVDLLTPILWHTAR
                     GQRLDQATSEDVIQTAWIRLVEGAEAIAEPQAVLAWLITTVRRESWRVLKRDGRSIPV
                     EEFPETEPDGLLAALDPAAEAMLREDQRVLWRAVSSLSERCQALLRIIAFIDRPDYGA
                     VSEALGMPVGSIGPTRGRCLAKLRGQLAADPTWSTP"
     gene            2765..3271
                     /locus_tag="PLK64_07470"
     CDS             2765..3271
                     /locus_tag="PLK64_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPV79621.1"
                     /translation="MTPEDRSSVADGPLDGTDTLVLGAVRELFSRTDPMPSGLTDRIK
                     FELTLAALHAEVAELQELTHTGVRADSASFTPTESVTFTSSTLSLMVSISPAGEGARP
                     GTVRIDGWVTGGPATVELRAGEKSIAAEADANGRFSVDAVPRGPVRFILRPVDARGRP
                     VITPVVEI"
     gene            3272..5896
                     /locus_tag="PLK64_07475"
     CDS             3272..5896
                     /locus_tag="PLK64_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HPV79622.1"
                     /translation="MTDATDTQPRAVAEAESLRRQAVSAIEDYQPDLAASLLDQAWEL
                     LEDLPRACAALPEACETRARIRLAQSWTTFEREGQVAAAPVLADALDLARAQDRLDLV
                     ALCLMQGATMSGRSGDLPGALTLMRQAEAGLTLLPLPDQVRLVLNRGLIAAQVGQLDD
                     ARDDLGRAADLAARAGAPPMEFMARHNRGYVEYLRGDLPAALSLMESADAMDVAVSRS
                     VSLLDQARVLLEAGLLDEAAHSLRRARDLASAEGGGQDLGEIELDLARTLLLLGDPHG
                     SAELAGQARRRFHDRAAAPWRRQAQLVELEAGSAARDHPERTARIAAALAEAASRHGE
                     VHVCRRARLIEADARLDLGEIEPARAAYTLARPLLTSASLPTRLHLRLVAARLATADP
                     GSPTRRATRHLTEAADDLARTQRRVSSLDVRTALAVHSNRLALLDLGIGVEQGSPASL
                     LARTERWRAVSDRVPLVRPPRDSRAADLLTQLRRTREDLRGAPAEAQVDLRSAATDLE
                     RRIRALDWARSDSGTPDWRPARPVSYPAALAAVRAADATLVSYVPHGPDLYAVCLTPR
                     GGRVLRVARSAEVSGATRRLRADVEAVSLPSLGPMQSAVEASLATQLAALDRLLIGPI
                     AANSARSGSRSGSRSGSGSDLLNRLIIVPSRALSGIPWGMLPSRRGRPTTVARTASAW
                     VGAQALGPMRSPRVLAIAGPDLQHADSEAAAVAEIWGGALVPSAEARLGAVVDALADR
                     DVVHIAAHGRHRHQSPLFSTLRLADGPAFAYELPDAGVTASHIVLSACEVGRGTVRDG
                     DETLGLAAVLLSMGVRTVVAAVSRIPDELAAGAMTAYHRLLVTGIDSATALAQATADL
                     PTVARAFTCFGADWRAAT"
     gene            complement(6005..6565)
                     /gene="cysE"
                     /locus_tag="PLK64_07480"
     CDS             complement(6005..6565)
                     /gene="cysE"
                     /locus_tag="PLK64_07480"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HPV79623.1"
                     /translation="MIREDIDAAMLRDPAAHSRVEMFLASSGLHAIWAYRVAHSLWGN
                     PSTRLAARLLSQIVRAMTGVEIHPGATIGRRFFIDHGMGVVIGETTEIGDDVMLYHGV
                     TLGGRSLSKTKRHPTLGNRVTVGAGAKVLGPILLGDDSQIGANSVVVKDVPAGYVAVG
                     MPAQVRPAVAGLTPEEMLDHNPAIWI"
     gene            complement(6744..7676)
                     /gene="cysK"
                     /locus_tag="PLK64_07485"
     CDS             complement(6744..7676)
                     /gene="cysK"
                     /locus_tag="PLK64_07485"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HPV79624.1"
                     /translation="MPIYDDVTALIGRTPLVRINRLFPDSVATVAAKLEFYNPANSVK
                     DRIGVAIIDAAEASGELPPGGTIVEATSGNTGIALAMVGAARGYNVVLTMPESMSKER
                     RALLRAFGAELILTDPSTGMKGAVTKAEEVAAERKGAVLARQFANAANPEIHRRTTAE
                     EIWADTDGGVDIVVAGIGTGGTITGVGQVLKARKPGVQIVAVEPAESPILNGGTPGPH
                     KIQGIGANFVPEILDTTVYDEVIDVNAGQAVDYARRAATQEGLLVGISSGAALYAAGL
                     VAARPENAGKTIVVIIPSFGERYLSTVLFEGLVD"
     gene            complement(7706..8035)
                     /locus_tag="PLK64_07490"
     CDS             complement(7706..8035)
                     /locus_tag="PLK64_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79625.1"
                     /translation="MCQLPAHPAGREHTEYQWSENLAAMSTRHRATPDHWYSVCGGGW
                     QRGQAAGVGMPRAAAGRRTQRRYAVRLGERGQVSGRHTGWWTGLRDGITFGWNIHNAS
                     DVLGGDP"
     gene            8062..8367
                     /locus_tag="PLK64_07495"
     CDS             8062..8367
                     /locus_tag="PLK64_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HPV79626.1"
                     /translation="MIGFVAGAAVGYVLGTRAGRQRYEQIKRGSARIWHSDPIQHRVE
                     AAGQAVKTQAVPFVADKVGDAVKAAGRAVKDKARPALPATVAVQDDGTEVAATDLGR"
     gene            complement(8436..9488)
                     /locus_tag="PLK64_07500"
     CDS             complement(8436..9488)
                     /locus_tag="PLK64_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPV79627.1"
                     /translation="MSATIRLQPSGHEVICDSGDTVLSALEKAGYIMPNNCRAGACGE
                     CKTKVLDGAFDQGMVLDMALSQEDRANGYGLMCMAKPLSPSLDIEFGTIDALQKLFPP
                     REFVPFVLTDKILRTPTMVELQLRPLGENLRFWPGQYVMLGDERAGAPLRSYSIANAP
                     RPDGEIQLLITKVEGGRTSAWVHDTLAVGDQLLLHGPYGTFVGDPSVETPVLCLASGS
                     GLAPILGLADGALRRGFKWGTTLIFSARTQDDVFHQGLMAWWGRKHRTFRNIVTYTGS
                     QPPEGAEFTGRIPAMLGQLFPTLEKHSIFIAGNPEFVEACKVACLQLGAKPDFVHVEG
                     YFPHWPAEVPPAERLS"
BASE COUNT         1346 a         3544 c         3387 g         1475 t
ORIGIN      
        1 tgctgatccc gccgtgcagg ctcggatagg cggtggcgtc gaccaggccc acgacatcgt
       61 cgagcttgac catcgccgac tggagctcga tctcgagctc ctggacatcc gtgggcaccc
      121 cgtcgatctc cttggggatg agctgacccc tggcgatccg ggctcccggc ttcttcttct
      181 ggacgaagac gacgatggac agttgccccg tgggtttgcc gtcgctgacc ttctcggcga
      241 tgtcgacacc gaccacgccc tcgcgcgcga gtaggtcgtc ctcgatcgac tccttcaccg
      301 ggcggatcaa cgcccgggtc tcctgctgtg catccatgtc tggtgctcct tgagctgggg
      361 agagagtggt caccccgaac gggctgacac gtccggaaga gcccccgccg acgccccaga
      421 tacccgctgg cgccgattgg ctcatgtgag gggacctgtc cggtatctgg cacggtgccc
      481 ccgactcctc accgatgagc accccgaccc ctaggctcgg agatgaggcc acgcatctcc
      541 cgtgaggacc tgaccatgag cgccatccag ccccccgccc agcccccagc ggcgacgtcg
      601 ctgcctgcgc cgtccgcgat cccgccgacc cctcagctgt cgcccgaggt ggcccagcgc
      661 tacggcgcgc ggatcctcga cccggtgacg gggatgcgtc gcagcgacgg cagcgccccg
      721 gctgccaccg tctatgtcgg cgaacgactc ctcatgcgca ccacccctgg ccgggccctc
      781 gatgacacgg tgcaggcgct caacgaggtc accaaggatt tcggggtccg cgccgagcgc
      841 atccccaccc cgccggcgaa gggtgagcgt gccctgcacc cggaggcgat ggcagtgctg
      901 caacggcatt gggtcgacgg cgtgcgactc ctccccgtgg atgaccaggt tcgtatgccg
      961 gtcgacgcgt ggccggtgct ggcggccgtg gccagccgcg acgccgcagc cgcagccccg
     1021 ctggccctcg atcacctcat gacgaccgca ccgtcgatcg gcggcacccc gtattggctc
     1081 gggaccccct actggctcgg caccccctat tggctgggga ccccttactg gctcggtacc
     1141 ggttcggctg ccggcgaata cggccggccc ggttcgggcg gacgcacccc tgtcagcgtg
     1201 gtgctccccg acccggcttt gactgcaccg cccgtcgggc gtcgccccgt cgtcgccgtc
     1261 cctgacaccg ggttgggcaa ccacccgtgg ttccccgcgg gcggcgccgc cacgagcgag
     1321 gtgcgttggg gcggcatact cctcgggatc aacgggcccg caggcggcgg ctacccgccc
     1381 gccccgcaga acccgctcga cccggtcatc gagagcgaca ccggccacgg caccttcatc
     1441 gccgggctga tccgccagtt gtgcccgcag gccgaggttc tcgctattcc ggtgatgcgc
     1501 ggcgacgggg tggtccccga gagcgtgctg ctcaacaccc tgaccctgct gttggcgcgg
     1561 catgtcgagg ccctggccac cggctcgacc gacggcctgg tcgacgtgtt gtcgctctcg
     1621 ctcggttact accacgagag cccggccgac gctgcgatgg atgccccgct gggggcgcta
     1681 ttgcgcgact tcggcgacca cggcgtcgcg gtgatcgccg cagcgggcaa ctcctcgacc
     1741 ctggccccga tgttccccgc cgggctgtcc gccgacgtca ctggtttctc gcctgacagc
     1801 gtgccgctgg ccagcgtcgg cgccctcaac cccgacggcg agactgtggc cttctactcc
     1861 aacgacggag actgggtgtc gaccttccgc accgggagct cgctggtcag caccttcccc
     1921 gtcgacgtca cggcatccgc gcaggcctcg gctcgggtcg acttcgccgg tcgggcgcgc
     1981 acgacccagg accccgacga ctaccggggc ggcttcgcgg cctggagcgg caccagcttc
     2041 gccgggccgg tcctcgccgg cgaggtcgcg gccgccctgg cccgcgacgc cgacctcgcc
     2101 accgtcgaca aggccgccgc tgtggcccga ggtcggcgcg ccatgttcgg tcaggtcacc
     2161 gagtggaagg gcaaggaatg acaccctccg caccacccac tttggccgac cgcgcggccg
     2221 ccgcgttcgc ggcataccgc gagggggatc gcgagcgcat gggtgagttg gtcgacctgc
     2281 tcaccccgat cctctggcac accgcacgcg gccagcggct ggaccaagcc acctccgaag
     2341 acgtcatcca gaccgcgtgg atccgcctcg tcgaaggtgc cgaagcgatc gccgagccac
     2401 aggccgtgct ggcctggctc atcacgacgg tccggcgcga gtcgtggcga gtcctcaagc
     2461 gcgacgggcg ctccatccct gtggaggagt tccccgagac cgagccggac gggctgctcg
     2521 ccgcactcga ccccgccgcc gaggcgatgc tgcgcgagga ccagcgcgtc ctgtggcgcg
     2581 cggtcagctc gctgtcggaa cgctgccagg cgctcctgcg gatcatcgcc tttatcgacc
     2641 gccccgacta cggggccgtc tcggaagccc tgggtatgcc ggtcggctcg atcggcccca
     2701 cccgcgggcg ctgtctcgcc aaactgcgcg gccaactggc tgccgacccc acctggagca
     2761 ccccatgacc cccgaggacc gcagcagcgt ggccgacggt cccctcgacg gcacggacac
     2821 cctggtgctc ggcgctgtgc gtgagctgtt cagccgcacc gatccgatgc ccagcggcct
     2881 caccgaccgg atcaagttcg agctcaccct cgctgcgctg catgccgagg tggctgagtt
     2941 gcaggagttg acgcacacgg gggttcgcgc cgacagtgcg tcgttcacac ccaccgagtc
     3001 ggtgaccttc accagttcca cgctcagcct catggtgtcg atcagcccgg cgggtgaggg
     3061 cgctcggccg ggcacggtcc ggatcgacgg ctgggtcact ggggggcccg cgacggtcga
     3121 actgcgcgct ggcgagaagt cgatcgcggc cgaagccgat gccaacggca ggttctcggt
     3181 cgacgcggtt ccacgcgggc ccgtgcggtt catcctgcga cccgttgatg ccaggggacg
     3241 gccggtcatt acccctgtcg tcgagatcta agtgacagac gccaccgaca cccaaccgcg
     3301 ggccgtcgcc gaggccgaga gcctgcggcg tcaggccgtc tcggctatcg aggactatca
     3361 gcccgatctg gccgccagtt tgctggacca ggcgtgggaa ctccttgagg accttccccg
     3421 ggcgtgtgcg gcgttgccgg aggcgtgcga gacccgagcc cggatcaggc tggcgcagag
     3481 ctggacgacg ttcgagcggg agggccaggt ggccgccgct cccgtcctcg ccgacgcact
     3541 cgacctcgcg cgggcgcagg accgcctcga tctggtcgcc ctgtgcctca tgcagggggc
     3601 cacgatgagc gggcgcagcg gcgacctgcc cggtgccctc accctcatgc gccaggccga
     3661 ggccggcctc accctgctgc ccctccccga ccaggtgcgc cttgtcctca accgcggcct
     3721 catcgccgct caggtgggcc aactcgacga cgctcgcgac gacctgggcc gggccgccga
     3781 cctggccgcg cgcgctgggg ctccgccgat ggagttcatg gcgcggcaca accggggcta
     3841 cgtcgaatac ctgcgcggcg acctccccgc ggccctgtcc ctcatggagt cggccgacgc
     3901 gatggacgtg gccgtcagcc gcagcgtctc cctgctcgat caggcccgag tgcttctgga
     3961 ggccgggctg ctcgatgagg ccgcccactc gttgcgccgg gcccgcgacc tggccagcgc
     4021 cgagggcggc ggccaggacc tcggcgagat cgagctcgac ctcgctcgca cgctgctgct
     4081 gctcggcgac ccgcacggct cggccgagtt ggccggtcag gcccgccgcc ggttccacga
     4141 ccgcgcggct gcgccgtggc gccgccaggc ccaactcgtc gagttggagg ccgggtccgc
     4201 cgcccgcgat caccccgagc gcaccgcccg catcgccgcc gccctcgccg aagccgcgtc
     4261 ccggcacggg gaggttcacg tatgccgccg tgcccggctc atcgaggccg acgcacggtt
     4321 ggatctcggc gagatcgagc cggcgcgggc ggcatacacc ttggctcggc cgctgctcac
     4381 ctcggcgtcg cttccgacgc gactgcactt gcgtctcgtc gccgcgcggc tggcgactgc
     4441 tgatcccggc agcccgacga ggcgggcgac ccggcacctg actgaggccg ccgacgacct
     4501 ggcccgcacc cagcggcggg tctccagcct cgacgtgcgc actgcgttgg ccgtccacag
     4561 caaccgcctg gccctgctcg atctcggaat tggggtcgaa cagggctctc ccgcttcgct
     4621 gttggcccgg accgagcgat ggcgggccgt gtccgaccgg gtcccgttgg tccgcccgcc
     4681 ccgagactcc cgggccgccg acctcctcac ccaactgcgt cgcacccgcg aagacctgcg
     4741 cggcgcccct gccgaggctc aggtcgacct gcgctcggcc gccaccgacc tggagcgccg
     4801 gatccgggcg ctcgactggg cccgcagcga ctccggcacg cccgactggc gacccgcgcg
     4861 tccggttagc tatccggcgg cgctcgccgc ggtgcgcgcc gctgacgcca ctctcgtcag
     4921 ctacgtcccc cacggccccg acctgtatgc cgtgtgcctc accccccgcg gcggccgcgt
     4981 gctgcgggtg gccaggtcgg ccgaggtgag cggagccacc cgacggctgc gggccgacgt
     5041 cgaagccgtc tcactgccga gccttggccc catgcagtcg gccgtcgagg cgtcgctggc
     5101 cacccaactt gccgcgctcg accgcctgct gatcggcccg atcgccgcca actccgctcg
     5161 ctccgggtcg cgctccggct cgcgctccgg ctcgggctcc gacctgctca accggctgat
     5221 catcgtgccg tcccgagccc tgagcggcat tccgtggggg atgttgccga gccgtcgggg
     5281 ccgcccgacc acggtcgccc gcactgcctc ggcctgggtc ggcgcccagg ctctcggccc
     5341 gatgcgctcg ccgcgggtgc tggccattgc cggccccgat ctgcagcacg ctgactccga
     5401 ggcggcagcc gtggccgaga tctggggcgg cgcgttggtg ccttcggccg aggcccggct
     5461 cggtgcggtc gtcgacgcct tggccgaccg ggacgtcgtc cacatcgccg cgcacggacg
     5521 ccaccgccac cagagcccgt tgttctcgac cttgcgcctg gccgacgggc cggctttcgc
     5581 ctatgagttg cccgatgccg gggtcaccgc gtcacacatc gtcctgtcgg cctgcgaggt
     5641 cggccgaggc accgtccgtg acggcgacga aaccctgggc ctggccgccg tgttgctgag
     5701 catgggcgtg cgcacggtcg tggcggccgt gtcgcgcatc ccggacgagc tcgcggcagg
     5761 ggcgatgacg gcataccacc ggctgttggt gaccgggatc gacagtgcca cggcgctggc
     5821 tcaggcgacc gccgatcttc cgacggtggc gcgggccttc acgtgtttcg gagccgactg
     5881 gcgcgccgcg acctgagttg ccctcacgcc ccaccttgcc ccgccccgcc ctgccgattc
     5941 gaggtattcc accatcatct ggcgacggcc gaatacctcg aaccggctgg gtcgcgaccg
     6001 acgctcagat ccagatcgcc gggttgtggt cgagcatctc ctcgggcgtg aggcccgcaa
     6061 cggcgggtcg cacctgcgcc ggcatcccga cggcgacata gcccgcaggg acgtccttga
     6121 cgaccaccga gttggcgccg atctgagagt cgtcgccgag gaggatcggg ccgaggacct
     6181 tcgcgccggc cccgaccgtg acccggttgc ccagggtggg gtggcgcttg gtcttcgaca
     6241 gcgaccggcc accgagcgtg acgccgtgat agagcatgac gtcatcgccg atctcggtgg
     6301 tctcgccgat gacgacgccc atgccgtggt cgatgaagaa ccgccgcccg atcgtcgcgc
     6361 cggggtggat ctcgacaccg gtcatcgccc gcacgatctg cgagagcaac cgcgcggcca
     6421 gtcgggtcga cgggttgccc cacagcgagt gcgccacccg ataggcccag atggcgtgca
     6481 gccccgacga cgcaaggaac atctcgaccc gcgagtgcgc cgccggatcg cgcagcatcg
     6541 ccgcgtcgat gtcctcacgg atcatcgccc aggtccggcg cacgatgtgt cggcgcggcg
     6601 cggcgaccgg tccgggcggc cccaccggct cgacccggga cgtgaggccc gtgtcggggt
     6661 cggcggtgag ctcccacgac cgatcgcggc cgcggtcacg gcgcagggtg ctggtcagtg
     6721 cggcgagcat ggcggtatgc cgttcagtcg acgaggccct cgaagaggac cgtggagagg
     6781 tagcgctccc cgaagctggg gatgatgacg acgatggtct tgccggcgtt ctcggggcgc
     6841 gcggcgacca ggcctgcggc atacagggca gcgccggagg agatgccgac aagcaggccc
     6901 tcctgggtgg cggcccggcg ggcgtagtcg acggcctgcc cggcgttgac gtcgatgacc
     6961 tcgtcgtaga cggtggtgtc gaggatctcc gggacgaagt tggcgccgat gccctggatc
     7021 ttgtgcgggc cgggggtgcc gccgttgagg atcggcgact cggccggctc gacggccacg
     7081 atctggaccc ccggcttgcg ggccttgagc acctggccga ctccggtgat cgtgccaccg
     7141 gtgccgatgc ccgcgacgac gatgtcgacg ccaccatcgg tgtcggccca gatctcctcg
     7201 gccgtcgtgc ggcggtggat ctcggggttc gcggcgttgg cgaactggcg ggccagcacg
     7261 gcgcccttgc gctcggcggc gacctcttcg gccttggtga cggcgccctt catgccggtc
     7321 gacgggtcgg tgaggatcaa ctcggcaccg aaggcgcgca gcagggcccg gcgctccttg
     7381 gacatcgact cgggcatcgt caggacgacg ttgtagccgc gggcggcgcc gaccatcgcc
     7441 agggcgatgc ccgtgttgcc cgaggtggcc tcgacgatag tgccgccggg cggcagttcg
     7501 ccggacgcct cggcagcgtc gatgatcgcc acgccgatgc ggtccttgac ggagttggcc
     7561 gggttgtaga actcgagctt ggcggccacc gtggccaccg agtcggggaa caaccggttg
     7621 atccgcacga gcggggtgcg gccgatcagg gcggtgacgt cgtcgtagat gggcatgctc
     7681 ggtggtctcc cgggtgtcgg ggcggtcagg ggtcacctcc caaaacatcg gaggcgttat
     7741 ggatattcca accgaacgtt atcccatcgc gcaggcccgt ccaccagccg gtatgccgcc
     7801 cgctcacctg gccccgctca cccagccgca cggcataccg ccgctgcgtc cgcctccccg
     7861 ccgcggcgcg cggcataccc acgcccgcgg cctgtccccg ctgccaccca ccgccgcaca
     7921 ccgaatacca atggtcaggc gtcgcccggt ggcgcgtgct catggcggcg agattctccg
     7981 accattggta ttcggtgtgt tcccggccgg ccggatgggc tgggagttga cacatggcgg
     8041 gaggctgggg agggtggggg catgatcgga ttcgtcgcgg gcgctgcagt cggctatgtc
     8101 ctggggacgc gggccgggcg ccagcggtac gagcagatca agcgcggcag tgcgcggatc
     8161 tggcactccg accccatcca gcaccgggtc gaggcggccg gtcaggcggt caagacgcag
     8221 gcggtgccgt tcgtcgccga caaggtgggc gacgctgtga aggccgccgg tcgcgccgtc
     8281 aaggacaagg cccgcccggc gctgcccgcg acagtggccg tgcaggacga cggcaccgag
     8341 gtcgccgcga ccgacctggg tcgctgaggg ctgcgccctc aggtcagttg acggaattgc
     8401 gccctcaggt cagctgacgg acgtgcgccc tcgggtcagc tgaggcgctc ggcgggggga
     8461 acctcggcgg gccagtgcgg gaagtagccc tcgacgtgca cgaagtcggg cttggcccct
     8521 agttgcaagc aggcgacctt gcacgcctcg acgaactcgg ggttaccggc gatgaagatg
     8581 ctgtgcttct ccagggtcgg gaagagctga ccgagcatcg ccgggatccg gccggtgaac
     8641 tcggcccctt ccggcggctg cgagccggtg taggtgacga tgttgcggaa cgtccggtgc
     8701 ttgcgccccc accacgccat caggccctga tggaagacgt cgtcttgcgt gcgcgccgag
     8761 aagatcaggg tcgtgcccca tttgaagcca cggcgcagcg ccccgtcggc cagcccgagg
     8821 atcggcgcga ggcccgatcc ggacgccagg cacagcaccg gagtctcgac cgaggggtcg
     8881 ccgacaaacg tgccataagg cccgtgcagc agcagttggt caccgaccgc cagggtgtcg
     8941 tggacccatg ccgacgtccg gccgccctcg accttggtga tgagcaactg gatctcgccg
     9001 tcggggcggg gagcgttggc aatcgaatag gaccgcaggg gagcgcccgc ccgctcgtcg
     9061 ccgagcatga cgtactgccc cggccagaag cgcaggttct cccccaacgg acgcaactgc
     9121 aactcgacca tcgtcggcgt ccgcaggatc ttgtcggtga ggacgaacgg cacgaactcc
     9181 cgcggcggga acagcttctg cagcgcgtcg atcgtgccga attcgatgtc caggctcggg
     9241 ctcagcggct tggccatgca catgaggcca tagccgttgg cccgatcctc ttgcgagaga
     9301 gccatatcca gaaccatgcc ctggtcaaac gccccgtcga gcaccttggt cttgcactcg
     9361 ccgcacgcgc ccgcacggca gttgttgggc atgatgtagc ccgccttctc caaggccgag
     9421 agcacggtgt cgccggagtc gcagatgacc tcgtggccgg agggctgcaa gcggatggtc
     9481 gcggacatcg ttccttcttg tcagacgcgc gtcgggcggc ggtcagcggg tgtgtggggt
     9541 gaccaacagg tatgccgcgc ggccgcagag ggtcggccgc gcggcatacc aaatcctgtg
     9601 tggtggagtc aggtcagaag acggggatga tgtcgtcgag gtcgacgtcg ctgacctctt
     9661 cgccggagat gtccacctcg gggatggccg ggaaggggat cgagtagcgg tccaggacac
     9721 ccttgaggtt gaggatggcg ttgcgccagt tc
//
LOCUS       DAOMZY010000105         9747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_6586_length_9747_cov_5.641251, whole genome shotgun sequence.
ACCESSION   DAOMZY010000105 DAOMZY010000000
VERSION     DAOMZY010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9747
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6586_length_9747_cov_5.641251"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /locus_tag="PLK64_07505"
     CDS             <1..1277
                     /locus_tag="PLK64_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPV79628.1"
                     /translation="QSILFLLEANPGAGLGLLLAYMVFGKGMAKASAPGAALIHFVGG
                     IHEIYFPYVLAKPKLIIAMIAGGMTQIFVNVLFHSGLRAPAAPGSILAVYAQTPAASL
                     IGVTLSWILGAAATFFVAAFILKTDKSEDEGDLSRATADMEAMKGKKSIASATLASGA
                     GGTAVLTKPIRSIVFACDAGMGSSAMGASILRRKIQAAGFGDVTVVNKAIANLTDDVD
                     LVVSHQDLTPRARQRTGSAVHVSVDNFMGSPAYDEIVEMLGRTNAPGAAAAVVDAAVV
                     EDAPADTAELLTTRCITLSGTARTRDEAITEAGQLLVAAGAVDAAYVASMHDRERSVS
                     THMGSRLAIPHGTNEAKASIRRSAISFVRYPEGIEWNGKPTTFVIGIAGVGDEHLSLL
                     QRIAHVFLDKSKVAALEAATTPEQVQAILAAG"
     gene            1395..2273
                     /gene="htpX"
                     /locus_tag="PLK64_07510"
     CDS             1395..2273
                     /gene="htpX"
                     /locus_tag="PLK64_07510"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HPV79629.1"
                     /translation="MHNHYNGLKTAALFGAIWALLLGLGAVVSGGRYVWIFALIGLGT
                     TFYGYWNSDKLAIRAMRAYPVSEAQAPQMYRIVRELSTKAGKPMPQLFISPTDAPNAF
                     ATGRNPDHAAVCCTEGILNLLDERELRGVLGHELMHVYNRDILTSSVAAAIAGVITSV
                     AQMLMFFGMFGGGGSSDENRPNPLAMLGFALLAPIAASVIQLAISRTREFDADEDGSS
                     LTGDPLALASALRKLEVGVARAPLAPQQQVVNASHMMIANPFRAQDVSRLFSTHPPMH
                     ERVRRLEAMAGGIQGR"
     gene            complement(2331..5288)
                     /locus_tag="PLK64_07515"
     CDS             complement(2331..5288)
                     /locus_tag="PLK64_07515"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HPV79630.1"
                     /translation="MRVGVLGPLLIDGAGGETTPRGQRPRDVLAVLLQRRGHVVTPDV
                     LLDLVWGDAAVELTASTVHTVVARLRRALGSDVIETHDAGYRLGRQVRLDSDDFDAAL
                     RRAREATAAGDAAGAVTAYRDAVAVWRGPQAFEGVSDDLVMADRARLGEAHAQTLDEL
                     ALALLESGTAPALDEAWSLARALTREHPLREGSHRTAMLAAVRSGRQAEALDLYRALR
                     ARLRDELGIEPSPPTAALHQRILTQDPTLDPALDLGPTHEQRTGPEPQVTGERPPDQR
                     EMGRLPAPLTSTVGREEDLATVITLLEQGRRLVTILGPGGVGKSRLLAEVGGRLSADP
                     SLEVVYVDLSGLGATNAADIADAVAVGFRLPVSRDDPISSLVNHLCADRLVALVDEAE
                     WASTAVAEVASQILAGCPGVRLVVTSRVPLDVLGESRVLLAPLACPPEDASGAEARRA
                     PAVRLLEDRLRDHAPDLVIGDEQGADLARLARRVDGLPLALELLAGYAASRTLAELLP
                     LVDAPLDVASPEVGHRPRHRSLRDTLLWSVERLTPSERLVLRRLGVFAGSFDVSAARS
                     VVGGSLDPLTVDAGLRALVREALVQLDRRGPRSRLRLLRTVRDLALEGLDESGETQEV
                     RRRHREWFAARWRGQPPHDELIAEVGASYDDYLEALRQGLAARDACSVADLVIALGRR
                     WSFVEAAGVGVRWFDRVLAADLLDPVDTARVQVLRVGLTLHASWADSRHLIARCQEAL
                     ANDPDWLAQAWQVESIQAYQAGDVERSVTAADQMVTVARRGARWVLPEALATTAVMVA
                     AAGDREGALASADEAWALIGASPTALDFASVIPKIGLALVDADQPQRAYDILSRATTD
                     VSIRLGLRPTFTIVINLGWAALGIDRPAEALSWFARSLNSGSHRGLFLAEECVGAACA
                     LTSLGHPQAAALLDAADELSSRHALSLTPRMAAHRQAARYLQPPSGDRSLSSRTDEDL
                     AAAIGTAAREFAP"
     gene            complement(5345..8548)
                     /locus_tag="PLK64_07520"
     CDS             complement(5345..8548)
                     /locus_tag="PLK64_07520"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPV79631.1"
                     /translation="MTTQAPVSSFDITYQQPGIAGGIRVAAALHRDRLELRLSTGVLA
                     AFFAFPQLGRPHFPEAGNGSDPVMVLGPDRVTVTVVGLPSESAELVRAALADRIALVA
                     SGDPTTVIPLELGPSTPVDGGVGFPLLGRPAERQLYDVALRAGTVGWEVVAPHAVYYR
                     STWTDFGLAHITDTHVARRIDSFRPTLRDLGLTEAAARMCNMNDQFRGFVSFANRLHA
                     AGELDVIVATGDLIDYVHETDDDREGLGNAGFLRDLILGRAPGPDWPTVEELRVPILM
                     TPGNHDYRRHPYHLVFDVNLGGQDIQRVRNFSELALLEREAMALTNTLYFPGATEVPN
                     LGKSAATAMVEIDPTLRAFRQALADPGPHVARLGKHRVVLVDSAHDVGMPDSATDALW
                     ELVKEWWNGSGDEDFMTLIGGSPNCEGVNDEEYAVAVDAIESAPDDGLVVLGLHAPLI
                     NPWNGETPFFLRETQRPALAQQAAWWVQRHTGATSADLMSEHPDWFAPPGEGEPAYLK
                     RGTTQDLLDAGVSRGRTDDLLQALAGVGTRRRADVVLAGHTHRYNEISIRVLDDGTLS
                     YFLDFYTANPRAWYPNKVVRVGDVRQAAGGHLDLPTTKTYVEVDEDAIAHAEPHPMPW
                     DATHDWVTFVPPYADPLATSADPRAWWDRHKPLQLQTGALGLWENNQVSFSGLRLLSV
                     RGDVIQRVHFLPRERLDAYRWELSLEQAAAPEPRHQVLTRERTRRFGSPPAASAPLVL
                     TPAAGGNSVVYRDGEGYLVELWDVPGSAGAGRLAGRDVAPAAVGSPSGFVGPDGTAVV
                     LFRGDDRHIHSLYWAGTASAGHDALSQSCEASEAEGDPSGYVLAGITHVFYRTADGHI
                     EELWWPGAEAVRHGHITGYCDEPLAAGDPQGYPVTTTAQNIVLYRGVDGHVHSLYWSD
                     GPTGHDNLSGYCGSPLAAGDPFGYHLPHLDSHQVVYRSADGHLHEIGWAGAAPASAWD
                     VVGAAGAPPAAADPACWFVPANGTKHISYAGVDGHVHDLAWPAGTATPTWTDLTLSAL
                     APPAAAERVTGWVEPGSATCRVAFRGTDGHLHEIRWG"
     gene            complement(8712..>9747)
                     /locus_tag="PLK64_07525"
     CDS             complement(8712..>9747)
                     /locus_tag="PLK64_07525"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPV79632.1"
                     /translation="EAITVVFPDGAPERTDRPTLAAYGASLSVLPSVSRVDTPVGTYV
                     RGARASGPRPDLLSDSAAAVLVIPDVEVYSQAAQDLVTAIRRADAPAVPLVGGPTARF
                     VDVNASIASRLPLVGALIALTTFLVIFFFTGSIVLPVKALLTNAATIAAVLGVMVWVF
                     QEGHGSGLLGFTPTPLSVSIPPLMFCLAFGLSMDYEVFLLGRIKEARDRGLDDTAAVA
                     SGLGSTGRIVTAAAALMSVTFLAFSTSKVSFIQMLGLGCALAILLDATLVRGVLVPAL
                     MRLMGRWNWWAPAPLVRLHERIAPAPEGFPRPLGLARTGTDASRRNAGDARGEHHPNT
                     AGEAMSGTVR"
BASE COUNT         1485 a         3334 c         3505 g         1423 t
ORIGIN      
        1 gacagtcgat cctcttcctc ctcgaagcca acccgggcgc gggcctcggg ctgctgctcg
       61 cctacatggt gttcggcaag ggcatggcca aggcctcagc cccgggcgct gccctcatcc
      121 acttcgtcgg cggcatccac gagatctact tcccctacgt cctggccaag ccgaagttga
      181 tcatcgcgat gatcgccggt ggtatgaccc agatcttcgt caacgtcctc ttccactccg
      241 gcctgcgggc gccggccgca cctggctcga tcctcgccgt ctacgcgcag acccccgccg
      301 ccagcctgat cggtgtcacg ctctcgtgga tcctcggcgc tgcggcgacc ttcttcgtcg
      361 ccgcgttcat cctgaagacc gacaagtccg aggacgaggg tgacctcagc cgggcgacgg
      421 cggacatgga ggcgatgaag ggcaagaagt cgatcgcctc ggcgaccctc gccagtggtg
      481 cgggtggcac ggccgtgctc accaagccca ttcgctcgat cgtcttcgcc tgcgacgcgg
      541 gcatgggctc ctcggccatg ggtgcctcga tcctgcggcg caagatccag gcagcgggct
      601 tcggtgatgt gaccgtcgtc aacaaggcga ttgcgaacct caccgacgat gtcgacctgg
      661 tggtcagcca ccaggacctc accccgcggg cacgccagcg gacgggctcg gcggtccacg
      721 tgtcggtcga caacttcatg gggtcgccgg cctacgacga gatcgtcgag atgctgggcc
      781 ggaccaacgc gcctggggca gcggcagccg ttgtggatgc ggccgttgtc gaggacgccc
      841 cggccgacac cgccgagttg ctcaccactc gctgcatcac cttgtcgggc acagcccgca
      901 cgcgtgacga ggcgatcacc gaggccggcc agttgctggt ggccgcgggg gccgtcgacg
      961 cggcatacgt cgcctccatg cacgaccgcg agaggtccgt gtccacccac atgggcagcc
     1021 gtctggcgat cccgcacggg accaacgagg cgaaggcgtc gatccggcgc tcggcaatct
     1081 cgttcgtgcg ttaccccgag gggatcgagt ggaacggcaa gccgacgacc ttcgtcatcg
     1141 gcatcgccgg cgtcggggac gagcacctct cgctgctgca gcggatcgcg cacgtgttcc
     1201 tcgacaagtc caaggtggcg gcacttgagg ccgccactac gccggagcag gtccaggcca
     1261 tcctcgcggc cggctgatcg accgactctg tgggtcacgg tccgcgcgcc cttggggggg
     1321 cgcgcggacc gtccccgttt tgggggtcgc cgtcggtggg aacaacggtg cgtcgctgcc
     1381 tcgttgagcc agctatgcac aaccactaca acggtctcaa gaccgctgct ctcttcggcg
     1441 ccatctgggc gctgctgctc gggctcggcg cagtggtgtc cggggggcgc tacgtctgga
     1501 tcttcgcgct catcgggctc ggcacgacgt tctacgggta ttggaacagc gacaaactgg
     1561 cgatccgcgc gatgcgggcc tacccggtga gcgaggccca ggccccgcag atgtatcgga
     1621 tcgtccgcga gctgtcgacc aaggccggca agccaatgcc ccagttgttc atcagcccga
     1681 ccgatgcgcc caatgccttt gccaccggcc gcaatcctga tcatgcagcc gtctgctgca
     1741 ccgaggggat cctgaacctg ctcgacgaac gcgagctgcg cggggtgctc gggcacgagc
     1801 tcatgcacgt ctacaaccgc gacatcctca cctcgtcggt ggccgccgcc atcgccggtg
     1861 tcatcacctc ggtcgcccag atgctcatgt tcttcggcat gttcggtggc ggaggcagct
     1921 cagacgagaa ccgccccaac cccctcgcca tgctcggctt cgccctgctg gctccgatcg
     1981 ccgcctcggt catccagctc gcgatcagcc ggacccggga gttcgacgcc gacgaggatg
     2041 gctcgagcct caccggtgat ccgctcgccc tcgcctcagc gctgcgcaag ctcgaggtgg
     2101 gtgtcgctcg ggcgccgctc gccccgcaac agcaggtcgt caacgccagc cacatgatga
     2161 tcgccaaccc gttccgcgcc caggacgtct cgcggctctt ctccacccac ccgccgatgc
     2221 acgagcgggt ccgtcgcctc gaggcgatgg ccggcgggat ccagggacgc tgaacccacc
     2281 tgccgcgcaa cggcataagc cctcggtatg ccgtgcggct gggtccgcgg tcagggtgcg
     2341 aactccctgg ctgccgtacc gatcgcggcg gccaggtcct cgtcggtgcg gctgctgagc
     2401 gaccggtcgc ctgagggcgg ctgcaggtat cgggcggctt ggcgatgggc ggccatgcgc
     2461 ggcgtgaggc tgagcgcatg ccgggaggac aactcgtcgg cagcatcgag cagcgccgca
     2521 gcctgggggt ggccaagaga tgtcaacgca cacgccgccc cgacgcactc ttcggccaag
     2581 aacaaccctc gatgtgaccc tgagttcagc gaccgcgcga accagctcaa cgcctccgct
     2641 ggccggtcga tgcccagcgc ggcccacccc aggttgatca cgatggtgaa cgtgggccgc
     2701 aggcctagtc ggatgctgac gtccgtcgtg gctctgctga ggatgtcgta ggcccgttgc
     2761 ggttgatcgg cgtcgaccag ggccaggccg atcttcggga tgaccgaggc gaaatccagg
     2821 gccgtcggtg aggccccgat cagggcccaa gcctcgtcgg ccgaggcgag cgccccctcc
     2881 cgatcgccag ccgccgcaac catgacggca gtcgtggcga gagcctcagg caacacccac
     2941 ctggcccccc ttcgcgcgac cgtgaccatc tgatccgctg ccgtgacgct tcgctcgaca
     3001 tcacctgcct gataggcctg gatcgactcc acctgccacg cctgagcgag ccaatcgggg
     3061 tcgttcgcca gagcctcctg gcacctcgcg atgagatgcc tggagtcggc ccacgacgcg
     3121 tgcaaggtca ggccgacccg cagcacctgc acgcgggccg tgtccaccgg atcgagcagg
     3181 tcagcggcca acacccggtc aaaccaccgc acgccgactc cggcagcctc gacgaaggac
     3241 cagcgtcggc ccaacgcgat caccaggtcg gcgaccgagc aggcgtcgcg ggccgccagc
     3301 ccctggcgca gcgcttcgag gtagtcgtcg taactcgcgc ccacctcggc aatcagttcg
     3361 tcgtgcggcg gctgtccgcg ccaccgtgcc gcgaaccact ctcgatggcg gcgccgcacc
     3421 tcctgggtct cgccgctctc gtcgagcccc tcaagggcga gatcgcggac ggtgcgcagc
     3481 agccgcagtc gagatcgcgg gccgcgccga tcgagttgga cgagggcttc gcgcacgagc
     3541 gcgcgcaggc ccgcatcgac cgtcagcgga tccagcgagc caccgacgac ggaacgagcg
     3601 gcagacacgt cgaacgaccc cgcgaagacg cccaggcgtc gcaggaccag gcgttcgctc
     3661 ggggtcagcc gctcgacgct ccacagcagg gtgtcgcgca gacttcggtg gcgtggccgg
     3721 tgacccactt cggggctggc cacgtccagg ggtgcatcga cgaggggcag caactccgcg
     3781 agggtccgcg acgcggcata cccggcgagc agttcgagcg cgagggggag tccgtcaacg
     3841 cggcgagcca accgcgcgag gtcagcgccc tgctcatcgc cgatgacgag atccggcgcg
     3901 tggtcgcgca ggcgatcctc aaggagccgg acggccgggg cgcgccgggc ctcggcgccg
     3961 gaggcgtcct ccggagggca ggccagcggg gcgagcagca cccgactctc cccgaggacg
     4021 tccaggggga cgcgcgaggt gacgacgagc cggacgcccg ggcagcccgc gaggatctgg
     4081 gaggccactt ccgcgaccgc tgtcgaggcc cactccgcct cgtcgacaag cgcgacgagg
     4141 cggtctgcgc acagatggtt gaccaggctg gagatcgggt cgtcgcgaga caccgggagc
     4201 cggaagccca ccgccacggc gtcggcgatg tcggcggcgt tcgtggcgcc gaggcccgac
     4261 aggtcgacat agacgacctc cagcgaggga tccgcgctca gccgcccgcc gacctcggca
     4321 agcaggcgac tcttacccac cccgcccggc ccaaggatcg tcaccaatcg acgcccctgc
     4381 tccagcaggg tgatcaccgt ggcgaggtcc tcctccctgc ccaccgtcga ggtgagcggc
     4441 gccgggagcc gacccatctc ccgttggtcc ggcggccgct cgcccgtgac ctgcggctct
     4501 gggccggtcc gctgttcgtg ggtcgggcca aggtcgaggg cggggtcgag ggtggggtcc
     4561 tgcgtgagga tgcgttggtg caacgccgca gtcggtgggc tcggctcgat ccccagctca
     4621 tcacgcaggc gggcccgcaa ggcccgatag aggtccagcg cctcggcttg gcggccgctg
     4681 cggacggccg cgagcatggc cgtccgatgg ctcccctcgc gcaggggatg ctcccgggtc
     4741 agcgccctgg ccagggacca ggcctcgtcc agggcagggg ccgttcccga ttccaacagc
     4801 gcgagcgcga gctcgtcgag ggtctgggcg tgggcctcgc cgagcctggc tcgatcggcc
     4861 atgaccaggt cgtcgctgac cccttcgaag gcctgcggtc cgcgccacac ggcaactgca
     4921 tctcggtatg ccgtgaccgc cccggccgcg tcgccagcgg cggtggcctc gcgggcccgg
     4981 cgcagggccg cgtcgaagtc gtccgaatcc agccgcacct gccggccgag gcggtagccg
     5041 gcgtcgtggg tctcgatgac gtcactgccc agggctcgcc gcaaccgggc gacgacggta
     5101 tggaccgtcg atgccgtgag ctcgactgcg gcgtcgcccc agaccaggtc gaggagcacg
     5161 tcaggcgtga cgacatggcc gcggcgctgg agcagcacgg cgagcacgtc ccgcggccgt
     5221 tggccgcggg gcgtggtctc accgcccgct ccgtcgatga gcagcggtcc cagaacgccg
     5281 acacgcatcc gcccatgcaa tcacaggacg gttgcgtgtc gcgcccgaat tgtcgggacg
     5341 ttgctcagcc ccaacggatc tcgtggaggt gcccgtcggt gccacggaag gccacccggc
     5401 aggtcgccga ccctggctcg acccaaccgg tcacgcgctc ggcggccgcc ggcggggcga
     5461 gggcgctgag agtgagatcg gtccaggtcg gtgtcgcggt cccagccggc caggcgaggt
     5521 cgtggacgtg accgtcgaca ccggcatacg agatgtgctt ggtgccgttt gcggggacga
     5581 accagcaggc cgggtcagcc gcagcagggg gtgcgccggc agctccgacg acgtcccagg
     5641 cgctcgccgg tgcggcgccg gcccagccga tctcgtggag gtgcccgtcc gcggagcggt
     5701 agacgacctg gtgggagtcc aggtggggca ggtgatagcc gaacgggtca cccgcggcga
     5761 gcggcgatcc gcaatagccc gacaggttgt cgtgccccgt ggggccatcc gaccagtaga
     5821 gcgagtggac gtgcccgtcg acgccccgat agaggacgat gttctgggca gtcgtcgtca
     5881 ccgggtatcc ctgaggatct cccgcggcga ggggttcgtc gcaatacccg gtgatgtggc
     5941 catggcggac ggcctcggcc cccggccacc acaactcctc gatgtgcccg tcggcggtgc
     6001 ggtagaagac gtgggtgatg ccggccagga cgtagccgct ggggtcgcct tcggcctcgg
     6061 atgcctcgca cgactgcgac agcgcgtcgt gtccggcgct cgccgtcccc gcccagtaaa
     6121 gcgagtgaat gtggcggtcg tcgccgcgga acagcacgac cgcggtcccg tcggggccga
     6181 cgaatcccga cggcgagccg accgcggcag gagcgacgtc gcgcccggcg agccgcccgg
     6241 cccccgcgct gccggggacg tcccacaact cgacgaggta tccctcaccg tcgcgataga
     6301 ccacgctgtt cccgcccgcc gctggggtga ggaccagggg agcgctcgcg gccggcggcg
     6361 acccgaaccg cctggtccgc tcccgggtga gcacctggtg gcgcggctcc ggggccgccg
     6421 cctgctcgag cgacaactcc cagcggtagg cgtccaggcg ttctcggggg aggaagtgca
     6481 cccgctggat gacgtccccg cgcacgctga gcagccgcag cccgctgaag ctcacctggt
     6541 tgttctccca cagcccgagg gcaccggtct gcagttgcag cggcttgtgc cggtcccacc
     6601 acgcgcgcgg gtcggccgac gtcgccagcg gatcggcata cggcgggacg aaggtgaccc
     6661 agtcgtgggt ggcgtcccag ggcatcgggt gcggctcggc gtgcgcgatg gcgtcctcgt
     6721 cgacctcgac ataggtcttg gtggtcggga ggtcgaggtg gccgcccgcc gcttggcgaa
     6781 cgtccccgac ccgcacgacc ttgttgggat accaggcgcg tgggttggcc gtgtagaagt
     6841 cgaggaagta gctgagggtg ccgtcatcga ggacccggat cgagatctcg ttgtagcgat
     6901 gggtgtgtcc ggcgagcacg acgtcggccc gccgccgggt cccaacaccg gcgagcgctt
     6961 gcaacaggtc gtcggttcgc ccccgcgaga cccctgcatc caggaggtcc tgggtggtcc
     7021 cgcgcttgag gtatgccggt tcgccctcac cggggggcgc gaaccagtcg gggtgctcgc
     7081 tcatgaggtc ggccgaggtt gctccggtat gccgttgcac ccaccacgct gcttgctggg
     7141 cgagggcggg gcgctgggtc tcgcgcagga aaaagggcgt ctccccgttc catggattga
     7201 tgaggggggc gtgcagcccg aggacgacga gcccgtcgtc cggggccgac tcgatggcgt
     7261 cgacggcgac ggcatactcc tcgtcgttga cgccctcgca gttgggggag ccgccgatga
     7321 gcgtcatgaa gtcttcgtcg cccgagccgt tccaccactc cttgacgagc tcccacagcg
     7381 cgtctgtggc cgagtcgggc ataccgacgt cgtgcgcgct gtcgacgagg acgacgcggt
     7441 gcttgcccag tcgagcgacg tgtgggccgg ggtcggccaa tgcctggcgg aaggcgcgca
     7501 gggtcgggtc gatctccacc atcgccgtgg cggcggactt gccgaggttg ggcacctccg
     7561 ttgcgccggg gaagtagagc gtgttggtga gggccatcgc ctcgcgctcg agcagggcga
     7621 gttcgctgaa gttgcgcacc cgctggatgt cctgcccgcc gaggttcacg tcgaacacca
     7681 ggtgatacgg gtggcggcgg tagtcgtggt tgcccggagt catgaggatc ggcacgcgca
     7741 actcctcgac ggtgggccag tccggacccg gcgcccgacc caggatgagg tcgcgcagga
     7801 agcccgcgtt gcccaggccc tcgcggtcgt cgtccgtttc gtggacgtag tcgatgaggt
     7861 cgccggtggc gacgatgaca tccagctccc cggccgcgtg cagccggttg gcgaacgaca
     7921 cgaagccacg gaactggtcg ttcatgttgc acatgcgggc ggccgcttcg gtgagcccga
     7981 ggtcgcgcag ggtgggtcgg aaggagtcga tccgccgggc gacgtgggtg tcggtgatgt
     8041 gcgcgagccc gaaatcggtc cacgtcgagc ggtagtagac cgcgtgtggg gcgacgacct
     8101 cccagccgac cgtcccagcg cgcagcgcca cgtcatagag ctggcgctcg gccggtcggc
     8161 ccagcagggg gaagccgacg cctccgtcga ccggggtcga cggcccgagc tcgagcggga
     8221 tgaccgtggt ggggtcgccg gaagccacga gcgcgatccg gtcggcgagg gccgcgcgca
     8281 ccaattccgc actttccgaa gggagtccga cgactgtgac ggtcaccctg tcggggccga
     8341 ggaccatcac cgggtctgac ccgttgccgg cttcggggaa gtgcggccgc cccagttgcg
     8401 ggaaggcgaa gaaggcggcg aggacgccgg tggataggcg cagctccagt cggtcgcggt
     8461 gcaacgcggc ggcgacgcgt atgccgcccg cgattcctgg ctgctggtag gtgatgtcga
     8521 acgatgagac gggggcctgc gtggtcatgg gatcggctcc ttcgaggcga tgtggtcaga
     8581 aggtcgatgg gtcagagcgt ggggttgagt gagggggcgg gctgggccgc cggtcagagg
     8641 gggagagcct cacccggtcc gcggcttcgt ggacgggccg ggtgaggccg taagcacctt
     8701 ggtccgcggg gtcagcggac cgttcccgac atcgcctccc cagcggtgtt cgggtggtgc
     8761 tcacctcggg catcgcccgc attccgacgg gaagcgtcgg tgccggttcg ggcgagaccg
     8821 aggggtcgtg ggaaaccttc aggtgcgggc gcgatgcgtt cgtgcagtcg gaccagtggg
     8881 gcgggagccc accagttcca ccggcccatg aggcgcatga gggccggcac caggacgccc
     8941 cgcacgaggg tcgcgtcgag caggatggcc agggcgcagc cgagtccgag catctgaatg
     9001 aaggacacct tgctcgtcga gaaggcgagg aaggtcaccg acatgagggc cgcagcggcg
     9061 gtaacgatgc ggcccgtgct gccgagcccg gaggcaactg cggccgtgtc gtccagtccg
     9121 cggtcacgtg cctccttgat ccggccgagg aggaacacct cgtagtccat cgacaggccg
     9181 aaggcgaggc agaacatcag gggcgggatc gacaccgaca ggggcgtcgg ggtgaagccg
     9241 agcaggcccg acccgtgccc ctcctggaag acccacacca tcacgccgag caccgccgcg
     9301 atggtggctg cgttggtcag cagcgctttg acggggagca cgatggatcc ggtgaagaag
     9361 aagatgacca ggaaggtcgt cagggcgatg agggcgccga ccaggggcag tcggctcgcg
     9421 atcgaggcat tgacgtcgac gaaccgagcc gtcgggccac cgaccaaggg cacggccggg
     9481 gcgtctgcgc gacggatcgc cgtgacgaga tcctgagcgg cttggctgta gacctccacg
     9541 tcagggatga cgagaacggc tgcggcagag tcggatagca agtccggtcg ggggccgcta
     9601 gctcgggcac cacgcacgta cgtgccgacc ggagtgtcca ctcggctcac cgagggcagc
     9661 acggacaggc tggcgccgta tgccgcgagg gtcggacggt ccgtgcgttc cggggctccg
     9721 tcggggaaga cgacggtgat cgcctcg
//
LOCUS       DAOMZY010000106         9737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_6601_length_9737_cov_5.859843, whole genome shotgun sequence.
ACCESSION   DAOMZY010000106 DAOMZY010000000
VERSION     DAOMZY010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9737
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6601_length_9737_cov_5.859843"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="PLK64_07530"
     CDS             <1..1198
                     /locus_tag="PLK64_07530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPV79633.1"
                     /translation="AAVAVLVGAVVPSANAAQYDPTSSRGFTLAGHGYGHGRGLSQWG
                     AYGAATKGVGLVAILDFYYPGTTRSQLADAPIRVSLSTGSSTQVAVDALGSMAVTAGG
                     TKVTLPATHAATGRTISAWRLVPNPSGSGLRLDFTYTGAGGWVRDRVFSTATAEFTST
                     RGTLRLIRPDSSRREYRGSLRAVRNVLTLSTINVLPMESYLRSVVPAEMPASWPATAL
                     AAQAVAARTYAAYKRARATGASDICDTTACQVYAGKAAWSSSGSLLRAYEVTTTDSAI
                     TASRLRALRYGSGFALTEFSSSNGGYTVASSLPYQVAKPDPYDGAVRNTANSWTKSVT
                     LSALSDAWPSIGTAKALVVNSRDGNGQWGGRVTSVSIVGTHATITVTGARFTSALGLR
                     STWWRG"
     gene            1306..3126
                     /locus_tag="PLK64_07535"
     CDS             1306..3126
                     /locus_tag="PLK64_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HPV79634.1"
                     /translation="MSRPTPTDHPFRLAWAVVDCLVWAAALVGATYLRYELSFAPIDW
                     RGMAIAVIIAVILQIGFGFLLGPYAIGHMRGSFEEIVDLTKTVAATTATLCIPVFIAL
                     PILVPKSVPLIAGPFALVAMLGLRLLVRSWKTRNPVGVQPGATEDAQRVLVFGAGEGG
                     RQLVRAMVRDRSRTYLPVAIIDDDPRKQRLTVEGVRVRGTRDALEQVADEYGAEVLAI
                     AMPSADADLIRDLSDRADHVGMDVMVLPPVSDLIGRPSLGDLRNLDLADLLGRRAISL
                     DTSAIAATISGKRVLVTGAGGSIGSELCRQIMRFGPAALFPLDRDESALHAVQLSLKR
                     PGLFERDGSLLVDIRDAEAVKLMFADVRPDIVFHAAALKHLPLLEQFPYEAYKSNVLG
                     TLNVLAAAAACGVDTVVNISTDKAADPTCMLGYSKRVAERLTAGYAVDHPGRFVSVRF
                     GNVLGSRGSVVHAFTAQIERGGPVTVTHPDVERFFMLIPEACQLVLEAAAAAPGHGEV
                     MVLDMGRPQKIVDVARALIRMSGRSDVKIDFTGLRPGEKLTEDLVANAETITRTKNPL
                     VTSAQVIPLEEKLLPDRTVHDHASAMAWCRAHSVTVEPMP"
     gene            complement(3150..4652)
                     /locus_tag="PLK64_07540"
     CDS             complement(3150..4652)
                     /locus_tag="PLK64_07540"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HPV79635.1"
                     /translation="MSGTAAAPGDRLARHTAYNGVAQFLPQLVGLVTIPFLLGSLGVA
                     AYGVWALINTLVVVFVSLDGGVSVSAQRFFALYAAQRADDRAVRLTMSALALFLGLTA
                     ALVLLGGPFAQLVLAVTDLPVEVVAGAEFLLRRLGFIVGLLLTSNVLLGYLRAHNRFA
                     LIAVATALSQGALCLVLWLHRDALTLETMFYVLLAQLGTYAAVMAVGASRLLRSMRGR
                     FAGREQLREFWAYASRSIVVNVSGLALLQAGPLFVAAVAPIEQVGYLAVATQLASAVR
                     SFPMFALPPMLTVITQTFGSAGADAAVRKASSFNRVWVPIIVAYASVTAMGMWFVARG
                     FAGALPIAQAAAVILTVGNCFNLVTGVSTACGNAIGRPGLAARYSAVSATATVIVTGP
                     AAFVGGAVGAAVAVCLVQAGALIYFLRLLRLEVPDLDRGGSVMHLPSAVAAAAVAGAG
                     GWVSLSWGARSPASLLVALAAVGLGFLAYAALNWRWLGPLRALPGSSAGA"
     gene            4786..5742
                     /locus_tag="PLK64_07545"
     CDS             4786..5742
                     /locus_tag="PLK64_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79636.1"
                     /translation="MESPPESAQPIEPGAVRAGLICVLYGSATLPQIVIDFARTAPAV
                     VVDNSGDLHDDPAGLTVLRPGRNLGYSAGVNAGLAALPSGLDAVLVVNPDIVGSVEAL
                     HDLAGTVARSAAPVLAAPTGGQGTFGFLPRAAAPLVIVQYLLRSNWHPTPRHPDSRFL
                     SGAVLALNAPALDLLAPGGLLLRPDLFFMDDVDITDRARAHDVRVVEVPVSTLLTHEG
                     GTSMRRRPAVRIYFSRVSKVRYWQGRSWFWGTVLRGFFMAEAAAGEVVARRKDADASD
                     GSAAMGFALARQWLRTGASELDERILGNPETEVRGRPPEVPR"
     gene            5739..6998
                     /locus_tag="PLK64_07550"
     CDS             5739..6998
                     /locus_tag="PLK64_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79637.1"
                     /translation="MSLDRIGFALRTTTPAVLPVNALVPVVVALGVLRIPGVDWLYAA
                     LGLVLIARAGRFPRAGRRLVAVYTVCIGSALVATWADSSAQAFTTSYLWPVVAQALFA
                     FGVLAGCDPARQVRRLVGGLLGGFVALWVIGVAEIATGFKLIRVLSPESSMAEMAAAS
                     RWVTMAVFTNYNDYCLALAMLAVLLFAHLLFVPKVHPFVALARWIVLGTSAALVTIMG
                     SRGALLAGLLGAVIVAVIAMRAVRPRLVTPGRIGKAVLIVFPLALWLWTSPYVQDHST
                     ATREAILNNSLALWAADPWTALVGYGSAANYALVTDTAFPGLLMDPHNLLLEVALNFG
                     VAALAIAMWWWIDVLRNTITGRAQARSWADAGTLAIVIALPVMGVVSSRLLPYIYVTV
                     LVTAASLLHPDFSQNPGSARVSRLPNH"
     gene            complement(6984..7571)
                     /locus_tag="PLK64_07555"
     CDS             complement(6984..7571)
                     /locus_tag="PLK64_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79638.1"
                     /translation="MPALLVATLVFAGAFLISGRGTDARVVQVDARLAVLPASATRSP
                     GTLADLRVAAASYGATIESNQVLEAVAAAPGRGWTAAGVKAAVTVVVLTDSLLIDLKV
                     QSASIEDATAMANATIAALQQEAPRQLGLTVDGAAPKIAVVSVPEATLQVAGSSRSSL
                     QSLILAGLGALVAGFLAAAALSARADWTRRRSVIR"
     gene            complement(7766..>9737)
                     /locus_tag="PLK64_07560"
     CDS             complement(7766..>9737)
                     /locus_tag="PLK64_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79639.1"
                     /translation="GAGRGLMTPLWAFEQVAGPVAFVAVLWGLGFATAHLGGLRNRLF
                     TAALAVPLTVGAVAISTLVHIVVPQPFGWVTVVVVLGIVGAVLRLVRRGRPRVASRPD
                     GRSVAGLMAMLGVSSACAMLVIFLSSGGSWELVSQTWDAIFDANATRQVYETGLVNPL
                     LISDFAFPQPVHTFYPSTFHALGALYMHLSGADAVVATNVVAAVLAGTLWPSVAVIAS
                     WIVLGPRSPRPAIVVLLSGGFWALPWAPLGWGVLWASALAAVFVPIVVAGAVAFVSPN
                     ERLGRRAAGALVVGGVALIAALHPRIAAILALLLVGLGCWFVGNQVVNHVRRREWLRA
                     LALAGPAILGLLMLAQFLRRFGRTSSQFGVRVWPVERPVALEVLGYLANGPGGSIPQP
                     LTAVAILVGMTVAWRSKRLRALALLAGGAVLLDVLTATLRGVWAFDVIARFWYNDRVR
                     TMAVAAGSLVVVAAVGWQRLSSWWLTRTDARPSPNERAATYPSVAAAIVLAMGTGCAI
                     PYMSERYIQAASSPTLSLITPEEVAAFRRVAEIVPEGDRILNNANDGSALLYAYVNRK
                     PTLLIAGLAGSTPNSEYLRDQLILLEPSNICKLMRADGIRWIINNGRAYSNGVIDEAT
                     SPRLQIPPGFPLTTERMRMGHTVLFELTGCPA"
BASE COUNT         1726 a         3328 c         3138 g         1545 t
ORIGIN      
        1 agcggcagtg gctgtcctcg tcggagctgt ggttccttcg gcgaacgcag ctcagtacga
       61 tccgacgagt tcccggggct tcacgctggc cggacacggc tacggtcatg ggcgcggcct
      121 ctcgcaatgg ggtgcctacg gtgccgcgac caagggcgtg ggcctcgtcg cgatcctcga
      181 cttctactac cccgggacga ctcggtcaca gctcgccgac gcgccgatca gagtgtcgct
      241 gtcgacgggc tcatctaccc aggtggccgt cgatgcgctc ggctcgatgg cggtcaccgc
      301 tggcgggacg aaggtcacac tgccagcaac tcacgcggcc actggacgca ccattagcgc
      361 ctggcgtctg gtgccaaacc cgtcagggtc cggcctcagg ctggacttca cctacaccgg
      421 ggcgggcggt tgggttcgcg accgtgtgtt ctcgactgcg acagccgagt tcacctcaac
      481 gcgcgggact cttcggctga tccgcccaga ctcgagtcgc cgagaatacc gtggctcgct
      541 gcgcgcggtc cggaacgtcc tcaccctctc gaccatcaac gtcctgccca tggagagcta
      601 cctgcgcagt gtggtcccag cggagatgcc cgcgagttgg ccggcaaccg ctctggccgc
      661 acaagcggtc gcagcacgga cgtatgcggc atacaagcgg gctcgggcca ccggcgccag
      721 tgacatctgc gacaccacgg cctgccaggt ctatgccggc aaggcggcct ggtcgagcag
      781 cggcagtctg ttgcgggcgt acgaggtcac gacgacggac tcggccatca cggcctcccg
      841 cctgcgggcg ctgcggtacg ggagcggctt cgcattgacg gagttcagtt cttccaacgg
      901 cggctacacc gtggccagtt cactgcccta ccaggtggcc aagccggacc cctacgacgg
      961 ggccgtgcgc aacacggcca actcgtggac caagtcggta accttgagcg ccttgagtga
     1021 cgcgtggcca tcgatcggca ccgccaaggc gctcgtggtc aatagtcggg acggcaacgg
     1081 ccaatgggga ggccgagtga ccagcgtgag catcgtcggc actcacgcga cgattacggt
     1141 gaccggagca aggttcacca gcgccctcgg gctccgctcg acgtggtgga ggggatgaga
     1201 atcgacccca gcggaatgtt cgataccgtt ggctagtccg cctgcaccga gctggctagt
     1261 ccgcctgcac tgagccgtgc cgggccatga cccaggagcc gtccgatttc tcgtcccacc
     1321 cccaccgatc atcccttccg gcttgcttgg gccgtcgttg actgcctcgt ctgggcagcg
     1381 gctctcgttg gcgcgacgta tctgcgctac gagttgagtt tcgcgccgat cgactggcgt
     1441 ggcatggcca tcgcggtgat cattgccgtg attctgcaga ttggtttcgg attcctccta
     1501 ggcccctatg caatcggcca catgaggggg tccttcgagg agatcgtcga cctgaccaag
     1561 acggtggcag cgacaaccgc gacgctctgt atcccggtgt tcatcgcctt gccgatcctc
     1621 gtgcccaaga gtgtcccgct catcgccggg ccgttcgcgc tggtcgccat gctcggactg
     1681 cgcctgctcg tgcggtcctg gaagacccgt aacccggtcg gcgtgcagcc cggtgccacc
     1741 gaggatgccc agcgggtcct cgtcttcggc gctggcgaag gtggcaggca gctggtacgg
     1801 gccatggtcc gggaccgaag ccgcacctac ctaccggtgg ccatcatcga cgacgacccc
     1861 cgtaagcagc gactcacggt cgagggcgta cgggtgcgag ggacgcgtga tgctctggaa
     1921 caggtcgccg acgagtatgg ggctgaggtc ctcgctatcg cgatgccctc ggccgatgcc
     1981 gacctcatcc gtgacctcag cgaccgcgcc gaccacgtcg gaatggacgt catggtcttg
     2041 ccgccggtat cggacctcat cggccgtccg tccctcggcg acttgcgcaa tctcgaccta
     2101 gccgacctgt tggggcgtcg agcgatctcg ctggacacct cggctatcgc tgcgacgatc
     2161 tccggtaagc gggtgctggt cactggagcc ggcggctcga tcggctccga gctgtgccgc
     2221 cagatcatgc ggtttgggcc agcagcgctc ttcccgctcg accgagacga gtccgcgttg
     2281 catgcggtcc agctctcgct caagcggccc gggctgttcg agcgagatgg ctcattgctc
     2341 gtcgacatcc gcgacgccga agcggtcaag ctgatgttcg ccgacgttcg cccggacatc
     2401 gtcttccacg ccgcggccct caaacatctg cccctcttgg agcagttccc gtatgaggcc
     2461 tacaagtcca atgtgctcgg gaccctcaac gtcctcgcgg ccgcggcagc ttgtggcgtg
     2521 gacacagtgg tcaacatctc caccgacaag gcggccgacc caacctgcat gctcggctat
     2581 agcaagaggg tggctgagcg actcaccgct gggtatgccg tagaccaccc gggccgcttc
     2641 gtgtcagtcc gcttcggcaa cgtcctgggc tcaagaggct cggtcgtgca tgcgttcact
     2701 gctcaaatcg aacggggcgg tcctgtcact gtcacccacc ccgacgtcga gcgtttcttc
     2761 atgctcatcc ctgaagcgtg ccagctggtg cttgaggcgg ccgccgccgc gcccgggcac
     2821 ggcgaagtca tggtgctgga catgggccgg ccgcagaaga tcgtcgacgt tgcccgcgcc
     2881 ctgatccgca tgtcaggacg atcggacgtc aagatcgact tcacagggct ccgcccgggc
     2941 gagaagctga ccgaggacct cgtcgccaat gccgagacca tcacccggac gaagaacccc
     3001 cttgtcacca gcgctcaggt catccccctg gaggagaaac tcctcccaga ccgcacggtc
     3061 cacgatcacg cgtcggcgat ggcctggtgc cgcgcccact ccgtcaccgt cgagcccatg
     3121 ccctaagtac ccgagcggga acgcgtgaac tacgcgcccg cggaggaccc tggcagtgcg
     3181 cgaagtgggc cgagccaccg ccagttgagg gcggcatacg cgaggaagcc caagccgacc
     3241 gcggccagag cgacgagcag ggaagctggg ctacgcgctc cccacgacag ggatacccac
     3301 ccgccggcgc ccgcgactgc ggcagcggcg acggccgaag gaaggtgcat gacggacccg
     3361 ccccgatcaa ggtcaggtac ctcaagacgc aggagccgca agaagtagat cagtgccccc
     3421 gcctgcacca gacagaccgc tactgccgcc cccacagcac cgccaacgaa ggctgccggt
     3481 ccggtgacga tgacggtggc ggtggccgag accgcgctgt agcgcgcagc gagcccgggc
     3541 cggccgatgg cgttcccgca cgcggtgctg accccggtga ccaggttgaa gcagttcccc
     3601 accgtgagga tcacagccgc cgcttgggcg atcggaagtg ccccagcgaa gccgcgggcg
     3661 acgaaccaca ttcccatggc cgtcaccgag gcataggcga caatgatcgg cacccacact
     3721 cggttgaagg agctggcttt tctcactgcc gcgtcggccc ctgctgagcc gaaggtctgg
     3781 gtgatcacgg tcagcatcgg aggcaatgcg aacatcggga aactgcgaac ggccgatgcc
     3841 aactgcgtgg cgacagcgag gtatccgacc tgttcgatgg gggcgacggc cgcaacgaag
     3901 agtggtcccg cttgtaagag ggctaggcca ctgacgttca cgacgatcga ccgactcgcg
     3961 taagcccaga actcccgcag ctgctcccgt cctgcgaacc ggccccgcat cgagcgcaag
     4021 agccgactcg ctccgacggc catcacggca gcgtaggtac ccagctgcgc cagaaggaca
     4081 tagaacatcg tctccaacgt cagcgcgtct cggtgcagcc agaggaccaa gcacagggcg
     4141 ccttgggaca atgccgttgc cactgcgatc agtgcgaatc ggttgtgagc ccgtaagtaa
     4201 cccagcaaca cgttgctggt gaggagaagc ccgacgatga agcccaggcg gcggagtaag
     4261 aactccgcgc cggcgacgac ttcgaccggc agatccgtga ctgccaggac cagctgcgcg
     4321 aaggggccac cgagcaggac aagtgccgcg gtcagcccga gaaagagcgc tagggccgac
     4381 atcgtcagcc ggacggcacg atcgtcggca cgctgggcgg cgtacagggc gaagaagcgc
     4441 tgggctgaca cgctcacgcc cccatccagc gagacgaaaa cgaccacgag agtgttgatc
     4501 agagcccaca cgccgtaggc cgccacaccg agggaaccca gcaggaaggg aatcgtcacc
     4561 aggccgacga gttgtggcaa gaattgcgca accccgttgt atgccgtgtg ccgcgccaaa
     4621 cgatcgccgg gggccgctgc tgtacccgac acccgcatct ccctatgtca cgtggccaac
     4681 ggacactggc gcgaggctag cagaggtccc gcccgccccg gcgacgcggc cgacccacgc
     4741 ggcacagcta ggctgacctt gagcgatgct gaggaaggag ccagggtgga gtcgccccca
     4801 gagagcgcgc agcccatcga acccggtgcc gtccgcgctg ggctgatttg cgttctgtat
     4861 gggtcagcca ccctccccca gatcgtcatc gatttcgcgc gcaccgcccc agccgtcgtt
     4921 gtcgacaact ccggcgatct gcacgacgac cctgccggtc tcaccgtcct gcgcccaggc
     4981 cgcaacctcg ggtactcggc cggggtgaac gccggcctag ccgcgctgcc ctcgggacta
     5041 gacgccgttc tcgtcgtcaa ccctgacatc gtcgggtcgg tcgaggccct tcatgacctc
     5101 gcgggaacgg tggcccgatc ggcagcgccg gttctggcgg cgccgaccgg aggccaaggc
     5161 acgttcggct ttctccccag agccgccgcg cccctggtca tcgtccagta ccttctgcgc
     5221 tcgaactggc atccgactcc ccgacatccg gacagtcgat tcctctcggg ggcggttctg
     5281 gccctcaatg ctccagccct ggacctgctg gcccccgggg gcctgctcct gcgccctgac
     5341 ctcttcttca tggatgacgt cgacatcacg gacagagccc gtgcccacga cgtgcgcgtc
     5401 gtcgaggtcc cggtctcgac cctcctgacg catgagggcg ggacaagcat gcggcgaagg
     5461 ccggccgtgc gaatctattt ttcccgggtg tccaaagtcc gttactggca gggccgttca
     5521 tggttttggg gaacggtgct gcgcgggttc ttcatggcgg aggccgctgc cggcgaagtc
     5581 gtggcccgca ggaaggacgc tgacgcatcc gacggaagtg cagcgatggg cttcgcactg
     5641 gcgcgccaat ggctgcggac cggcgcctcc gagctcgacg aacgaatctt gggcaacccg
     5701 gagacagagg tgcgcggacg tccgcctgag gtgccccgat gagccttgac cgcatcggct
     5761 ttgccctccg aacgacaacg cccgcggtgc tccccgtgaa cgcgctcgtg cccgtcgtgg
     5821 tggctttggg cgtcctgcgc atccccggcg tcgattggct ctacgccgcc ctcggcctcg
     5881 tcctgatcgc gcgcgccggg cgattccctc gcgccggccg aaggctcgtc gcggtctaca
     5941 ccgtgtgtat cggctccgct ctcgtcgcca cctgggcaga ctcttccgcg caagctttca
     6001 ccacgagtta cctctggccg gtggtggcac aggccctgtt cgccttcggg gtcctcgcag
     6061 gctgtgaccc cgcgcggcag gtccgccgtc ttgtcggcgg cctgctcggc gggttcgttg
     6121 cgctctgggt gatcggggtc gcagagatcg ctaccggctt caagttgatc cgcgtcctga
     6181 gcccagaatc ctccatggcc gagatggcgg cggccagtcg ctgggtgacc atggccgtct
     6241 tcaccaacta caacgactac tgcctggcat tggccatgct ggccgtgctg ctgttcgcgc
     6301 acctgttgtt cgtccccaaa gtccacccct tcgtcgccct cgcccggtgg atcgtgctcg
     6361 ggacgtcggc cgccctcgtc accatcatgg gttcgcgtgg ggcacttttg gctggcctcc
     6421 tgggggcagt cattgtcgca gttatcgcaa tgcgcgccgt ccgccctcgc ctggtaacgc
     6481 ccggtcggat cgggaaagcc gtgctcattg tgtttccgtt ggcgctctgg ttgtggacga
     6541 gcccatacgt ccaggaccac agcaccgcaa ctcgagaggc gatcctcaac aactcgttgg
     6601 cactatgggc ggcagacccc tggactgccc tggtgggcta cggctcagct gcgaactacg
     6661 ccctggtgac ggacaccgcc ttccccgggc tgctgatgga cccacacaac ttgctcctcg
     6721 aagtcgccct caacttcggt gttgccgcgc tcgcgattgc catgtggtgg tggattgacg
     6781 tgctccgaaa caccatcact ggtcgggcgc aagcacgctc atgggccgac gcggggactc
     6841 tcgccatcgt tatcgcgctc cccgtgatgg gagtcgtctc gagtcgccta ctcccctaca
     6901 tctacgtcac ggtcctcgtc accgccgcca gcctcttgca cccagacttt agtcagaacc
     6961 cgggttcagc ccgcgtctcg cgcctaccga atcactgacc ggcgccgggt ccaatcagcc
     7021 cgagccgaca gcgccgcagc cgcgaggaat cctgcgacga gcgcgccgag cccggccagg
     7081 atgagcgact gaaggctgga acggctactt ccggccacct gcagggtcgc ttccgggaca
     7141 gacaccacgg cgatcttcgg cgcggcgccg tcgacggtga ggccgagttg tcgcggcgcc
     7201 tcctgctgaa gcgccgcgat cgtcgcgttg gccatggccg tcgcgtcctc aatgctcgcg
     7261 ctctgtactt tcaggtcgat gaggagtgag tcggtcaaga cgacgacggt gacggccgcc
     7321 ttgacaccgg cagccgtcca cccgcggcct ggcgcagcag ccaccgcttc gagtacttga
     7381 ttggactcga ttgttgcgcc ataggacgca gcagcgaccc gaaggtcggc caacgtgccg
     7441 ggggacctgg tcgccgaggc tggaaggacg gctagccggg cgtccacctg cacgacgcga
     7501 gcatcagtgc cgcggccgga gatcaggaag gcaccagcga agacaagcgt cgccacaagc
     7561 agtgctggca cagcgtatct ccgcaggccg ccgagatagc cgaggaggtc aactacgtcg
     7621 cttggcggtg ccactggcgt cgcaggggaa ccatcggagg tgttgttgac cgacgttgaa
     7681 ctctcatcac tgggcacggc gccaagggta cctcccgacg cgggggcgaa agtggagcgg
     7741 agtccccagg gcgcagggga gtcgatcagg ccggacaccc ggtgagttca aaaagcacgg
     7801 tgtggcccat tcgcattcgc tcggtggtca gcgggaagcc aggggggatc tggagtctgg
     7861 gagaggtcgc ctcgtcgatg acgccattag agtaggccct gccattgttg ataatccagc
     7921 gaattccgtc tgcgcgcatg agtttgcaga tgttggacgg ctccagaaga atgagctggt
     7981 cccgcaggta ctcggagttc ggggtgctcc cagcgagtcc agcgatcagc agcgtcggct
     8041 tacggttgac gtaggcgtac agcagcgcag acccgtcgtt ggcgttgttg aggatccgat
     8101 caccctcggg gacgatttcg gctacccggc ggaaggccgc tacctcttca ggggtgatca
     8161 ggctgagggt cgggctactg gccgcttgga tgtaccgctc ggacatgtag gggatcgcac
     8221 agccagtgcc catcgccaag acgatggctg cggcaaccga ggggtacgtc gcggcgcgct
     8281 cattggggga aggacgtgca tccgtccggg tcagccacca cgaactcagg cgctgccagc
     8341 cgacagcggc gacaacgacg agggagcccg ccgcgacagc catggtgcga acccggtcgt
     8401 tgtaccaaaa ccgggcaatg acgtcgaagg cccacacgcc ccgaagcgtc gccgtgagca
     8461 cgtcgaggag tacagctccc ccggccaaga gggcaagtgc acgtaacctc ttcgagcgcc
     8521 aagcgacggt cattcccacg agaatcgcaa ccgccgtgag tggttgcggg attgacccac
     8581 ccggaccgtt ggcgaggtag ccgagcacct caagagccac tgggcgctcc accggccaca
     8641 cccgaacacc gaactggctg ctcgttcgac cgaaccggcg caggaactgg gccaacatga
     8701 gaagccccaa tatcgccggc cccgccaggg ccagcgcccg cagccactct cgtcgtcgca
     8761 catggttgac tacctggttc ccgacgaacc aacacccaag tcccaccagc aacagcgcga
     8821 gtatggccgc gatccgcggg tgcagggcag caatgagcgc gacgcccccg accaccaacg
     8881 ccccggcggc gcgacgaccc aaccgctcat tcggagacac gaacgccacc gcgccagcga
     8941 ccacgattgg gacgaataca gcggcgagtg cggacgccca caagacgccc cacccgaggg
     9001 gcgcccaagg aagggcccag aaccccccgc tgagcagcac cacgatcgcc ggccgcggac
     9061 tccgagggcc taagacgatc catgacgcaa tcacggcgac gctgggccac aacgtcccgg
     9121 ccagcaccgc cgctactacg ttcgtggcca cgaccgcgtc agcgccgctg agatgcatat
     9181 agagagcgcc gagagcatgg aaggtcgatg ggtagaaggt gtgcacgggc tgggggaagg
     9241 cgaagtcgga gatcagcagg gggttcacca accccgtctc atagacctgg cgagtggcgt
     9301 tggcgtcaaa tatggcatcc cacgtctgag acacgagttc ccaggaaccg ccgcttgaca
     9361 ggaagatcac gagcatcgcg caggcggacg agacccccag catggccatg agcccggcca
     9421 ccgaccgtcc gtcgggtctg gacgccacac gggggcggcc tcgacggact agccgcagaa
     9481 ccgctccaac aattcccaac acgacgacca ccgtcaccca cccgaaaggc tgaggcacga
     9541 cgatgtgtac gagagtgctg atcgcgaccg cgccaaccgt cagcgggacc gcaagcgcgg
     9601 cggtgaacag ccggttgcgc aggccgccaa ggtgggccgt cgcgaagccc agcccccaga
     9661 ggacagcaac aaaggccacg ggccccgcaa cctgctcgaa cgcccacaag ggagtcatca
     9721 ggcccctgcc cgcccca
//
LOCUS       DAOMZY010000107         9611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_6725_length_9611_cov_5.753035, whole genome shotgun sequence.
ACCESSION   DAOMZY010000107 DAOMZY010000000
VERSION     DAOMZY010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9611
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6725_length_9611_cov_5.753035"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PLK64_07565"
     CDS             complement(<1..254)
                     /locus_tag="PLK64_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79640.1"
                     /translation="MTNEQLRMVAHLNFSSRTLDGGLRAPSQRFDISQAAQDVLIHDF
                     DSSTVLAVDPATVSASAPIRVAGVQVAQGGSVLAVSDPAAG"
     gene            complement(654..1073)
                     /locus_tag="PLK64_07570"
     CDS             complement(654..1073)
                     /locus_tag="PLK64_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HPV79641.1"
                     /translation="MKPAPNSRRIAGMPPTRSAGDDRDPALLGAQLERLLLDRGWSAD
                     VTVGSVMGRWAEIVGAEIAGHVRPVTFEGTVLSVQADSTAWATQIRLLTSSLLGRIEA
                     VVGAGIVTDLVVLGPAAPSWVKGRRRVQGPGPRDTYG"
     gene            complement(1246..2457)
                     /gene="recF"
                     /locus_tag="PLK64_07575"
     CDS             complement(1246..2457)
                     /gene="recF"
                     /locus_tag="PLK64_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HPV79642.1"
                     /translation="MHVRHLSLVDFRSYAVAELPLAPGVTTIMGLNGQGKTNLVEAIG
                     YVATLGSHRVATDQPMVRFGAAQTVIRAAIVRHDRETLVELELTPGRANRARLNRAPL
                     TRTRDVLGTLRTVLFAPEDLALVKGDPAGRRAFLDELLVARQPRWAGVRADYERILKQ
                     RNALLKSAAPVLRKGSSRRRSASLAPGENPDTATESTLHTLDIWNTHLAEVGAQLLYA
                     RLRLLRDLTPFLAAAYATVSAGVTPVSASYRASLGESVTAAIGAGEVPDLEDLRAELL
                     AAFEANRAAEVERGVTLVGPHRDDVHLALGPMPAKGYASHGESWSLALGLKLAAYQLL
                     RRDLGEDPVLVLDDVFAELDTQRRERLAELVADCEQVLVTAAVGADVPERLRSTGRTY
                     AVTLGEVTRVD"
     gene            complement(2486..3508)
                     /gene="gnd"
                     /locus_tag="PLK64_07580"
     CDS             complement(2486..3508)
                     /gene="gnd"
                     /locus_tag="PLK64_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HPV79643.1"
                     /translation="MQLGLIGLGRMGANMRQRIRAGGHEVIGYDKNPAVRDVASLATM
                     VKKLSGPRVVWIMVPAGAPTRDTVAKLAGLLEKGDLVIDGGNSRFTDDFANAKLLAQK
                     GIGYVDCGVSGGVWGLENGYGLMAGGDKKWINLAMPIFDALRPEGERDLGFVHAGEVG
                     AGHYAKMVHNGIEYAMMHAYGEGYELLAKKDIVTDVPGCFKAWSRGTVVRSWLLDLLV
                     DALEDNPTLADVSDYTNDSGEGRWTVEEAIDNAVPMPVIAASLFARFASRQPVSPALQ
                     AVAALRGQFGGHAVMTVAEGENLRATAPESAGAKASPGQAPAAAKVAAKKTTTRRAAP
                     KASPAH"
     gene            complement(3715..4848)
                     /gene="dnaN"
                     /locus_tag="PLK64_07585"
     CDS             complement(3715..4848)
                     /gene="dnaN"
                     /locus_tag="PLK64_07585"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013490886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPV79644.1"
                     /translation="MKFRVEREVLADAVAWVARGLPNRPPVPVLSGVLIEADRAGTVT
                     LSAFDYEVSAKVTVAADVDEGGTVLVTGRLLSEISRALPARPVDVSTEGNKVTVTCGT
                     SRFSLLMMQADDYPTLPAAPQPSGTIAGDVFTQAVHQVAIAADRGDTLPILTGVRMQI
                     EGDKVTLLATDRYRLAMRELTWAPARSDAAYTALIPARTLSDTAKALGAAGSVELALG
                     GNAGGDGLIGFEAGQRRATTRLLDGEYPKVTSIFPTSVDSEAVLETGALIEAVKRVAL
                     VAERNTPVRLRFTDGQVAIEAGTGDDAQASEAVESTLTGPDIEIAFNPHFLLDGLGVI
                     GTPFTRFSFTQPSRPAVMAGQREATGEADTSYRYVLMPVRFAS"
     gene            complement(5360..6832)
                     /gene="dnaA"
                     /locus_tag="PLK64_07590"
     CDS             complement(5360..6832)
                     /gene="dnaA"
                     /locus_tag="PLK64_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPV79645.1"
                     /translation="MTGGDVDLTDLWQRTLVALERTGVSPSERAFVGFARLMAVVEQT
                     ALIAVPNDYTKKVLESDVREHLVATLSGALGRDIGLAVTVDPTLEHATPSSNGVDPAN
                     GFDTANGDERHESPDAARMERFHPEGSGRHTPGVIAFPRRDGDQDRSRLNPKYTFDTF
                     VAGSSNRFAHAAANAVSEAPAKAYNPLFIYGSSGLGKTHLLHAIGHYVRQIYPGFRVR
                     YVNSEEFTNEFINSIRDDRASTFQSLYRDVDVLLIDDIQFLAGKVQTQEEFFHTFNAL
                     HNNSKQVVITSDLPPKQLSGFEERLRSRFEWGLITDVQAPDLETRIAILRKKTLQERM
                     SAPDEVLEYIATRITSNIRELEGALIRVTAFASLNRQPVDFTLAEVVLKDLVPTDQPV
                     QITIGTIQAETASYYALTVDDLCSSSRSRQLVTARQIAMYLCRELTELSLPKIGQAFG
                     GRDHTTVMHADRKIRELMSERRAIYNQVTELTARIRNHSR"
     gene            7295..7432
                     /gene="rpmH"
                     /locus_tag="PLK64_07595"
     CDS             7295..7432
                     /gene="rpmH"
                     /locus_tag="PLK64_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013428281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPV79646.1"
                     /translation="MSKRTFQPNNRRRAKTHGFRLRMRTRAGRAILAARRRKGRSELS
                     A"
     gene            7442..7798
                     /gene="rnpA"
                     /locus_tag="PLK64_07600"
     CDS             7442..7798
                     /gene="rnpA"
                     /locus_tag="PLK64_07600"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPV79647.1"
                     /translation="MLPARNRLRESGQFRAVLRGPGGSRAGGPLMVVHAAMTDPMGAR
                     APRVGFVVSGAVGGAVVRNRVKRRLRALAGTALGGIPAGCDLVVRAAPAAATASFAEL
                     QAELDRCLRRALAKVA"
     gene            7803..8105
                     /gene="yidD"
                     /locus_tag="PLK64_07605"
     CDS             7803..8105
                     /gene="yidD"
                     /locus_tag="PLK64_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPV79648.1"
                     /translation="MTLAELLGPGLRGPWTWRKAAAAPLLMLVRIYQLAISPLLPQRC
                     RFYPSCSAYAVTALTRFGPLRGGWLAARRLGRCHPWNPGGVDHVPEREACRTHAAT"
     gene            8197..9252
                     /gene="yidC"
                     /locus_tag="PLK64_07610"
     CDS             8197..9252
                     /gene="yidC"
                     /locus_tag="PLK64_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HPV79649.1"
                     /translation="MGDFINLILSPLTIGEAWVMLAWHKVWTTIGIGGGWAWALSIVG
                     LTVVVRIVLIPLFVKQIHSSRRMQLIQPEMQKIQAKYKGKTDPESRQAMTQETMDLYK
                     KTGTNPFSSCLPILLQSPFFFALFTVLNNLKGIAEGTHPAIGPITPAVAHEIESSNIF
                     GAPFSSTFMGSTDTATKIVTVVLIILMSVSTFTTQRQLMRKNLPDSALDNPFMKQQTV
                     LLYLMPIFFALSGINFPIGVLLYWLTTNVWSMGQQFYVIRRMPAPGSAAERALHERQE
                     RKGKTVTPFSIRGLERDAAAGAAADETVQDAPNTVNRQRQQPKSKKRAKRSGPATGAA
                     GTDDAEGSAADGKPTDQ"
     gene            9310..>9611
                     /locus_tag="PLK64_07615"
     CDS             9310..>9611
                     /locus_tag="PLK64_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79650.1"
                     /translation="MSEAGQDVTELVEASEPVAEVDETVVDETVVNEVELDEVEVQGE
                     SDSGDLPVGDESDDDSDDDSDDDSDDDEESDDDSDEESGDEDDADGGDDEASGPSAG"
BASE COUNT         1520 a         3147 c         3377 g         1567 t
ORIGIN      
        1 cctgctgcgg gatcactcac agcgaggacg gatccacctt gcgccacctg aactccggcg
       61 acccgaatgg gagcgctggc agacaccgtg gcgggatcaa ccgcgagaac cgtggaggag
      121 tcgaagtcgt ggatgaggac atcctgcgcg gcttgggata tgtcaaagcg ctgcgacggg
      181 gcacgcaagc ccccgtccag cgttcgagag gagaaattga ggtgggcgac cattcgcagc
      241 tgttcattgg tcacccagac gcctccgtca tgaagggaga catcggacac tgggacaccg
      301 gggttgatcg acgccaagat tccgatgatt ccgacggaga cggtggccag cgcagtccgt
      361 ccccgcggct tgaatgcttc gcctagaccc accctgactc ccctacgttg ttcgcgcacg
      421 gcgcatcgtc gtcgcgaggt gcgctctcac gtgcactgca catagaagca cttcattgag
      481 gccccaacca cattgatacc gcagaatggg cagaactatc cgtccgcaac ccaacgatgc
      541 gctccgctca ttgccccgct tccatgcctc cgagactccg gccgaagacc cggagtcccc
      601 tcgactgata cctgacggac gagtgagtgc gaatctccga gtcaacgagt cggtcagccg
      661 taggtgtcac gggggccggg gccctgcacg cggcggcggc ccttgaccca cgaaggggcc
      721 gctggcccga gtacgaccag gtcggtgacg atgccagcgc ccaccaccgc ctcgatccgg
      781 cccagcagcg acgaggtgag cagccggatc tgggtcgccc atgccgtcga gtcggcctgg
      841 acgctgagga cagttccctc gaaggtcacc ggccgcacgt gcccggcgat ctccgccccg
      901 acgatctcgg cccaccgacc catgaccgag cccacggtga cgtcggcgga ccagccgcgg
      961 tcgagcaaca gtcgctccaa ctgcgcaccc agaagcgcgg ggtctcggtc gtccccggcg
     1021 ctgcgggtgg gcggcatacc agcgatgcga cgggagttgg gagccggctt catccctggg
     1081 cgaaaaccct tgtcacgtgc ggctttacgc gctcgggcca gtgcactgcg agaggcctcg
     1141 gccgggtcgg tgcccggcag gccctcagcg tctggctcag tgtccggctc cgtgtccctg
     1201 cccgcctcca cgtcggagtc cggtacggag agcgggcccg cctcgtcagt cgacacgggt
     1261 cacctcgccg agcgtgacgg catacgtgcg gccggtggag cgcagtcgct cagggacgtc
     1321 cgcgcccacg gcggcggtga cgagcacctg ctcgcagtcg gcgacgagct cggccagccg
     1381 ctcgcgtcgc tgggtgtcca gctcggcgaa cacgtcgtcc aggacgagca ccgggtcctc
     1441 gccgaggtcg cgccgcagca gttggtatgc cgcgagcttg agtcccagcg ccagcgacca
     1501 ggattcgcca tggctggcat agcccttggc cggcatcggc cccagcgcca ggtggacgtc
     1561 gtcgcgatgc ggcccaacga gggtgacccc tcgctcgact tcggcggccc ggttggcctc
     1621 gaaggccgcg agcaactcgg ctcgcagatc ctcgaggtcg gggacctcac cggcaccgat
     1681 ggctgcggtc accgactcac ccagactcgc gcggtagctc gccgacaccg gggtgactcc
     1741 cgcgctcacc gttgcgtatg ccgccgcgag gaagggggtg aggtcgcgca acagccgcag
     1801 ccgggcatag agcagttggg cgccgacctc ggcaaggtgg gtgttccaga tgtccagggt
     1861 gtgcagggtg gattccgttg ccgtgtcagg gttttcgcca ggggcgaggc tggccgaccg
     1921 gcgccgggac gagcccttgc gcagcactgg cgccgccgac ttgagcaagg cgttgcgctg
     1981 cttgaggatc cgttcgtagt cggcccgaac cccggcccag cggggctgcc gcgcgacgag
     2041 caattcgtcg aggaacgccc gtcgacccgc cgggtcgccc ttgaccagcg cgaggtcctc
     2101 gggcgcgaag agcaccgtcc gcagggtgcc gaggacgtcg cgggtccgcg tgagcggggc
     2161 ccggttgagg cgagcccggt tggcccggcc cggcgtcaac tcaagctcga cgagggtctc
     2221 ccgatcgtgg cggacgatcg cggcgcggat gaccgtctgg gctgccccga agcgaaccat
     2281 cggctggtcg gtggccaccc ggtgactgcc gagcgtcgcc acatagccga tggcctcgac
     2341 aaggttggtc ttgccctggc cgttgaggcc catgatcgtt gtgacacccg gagcgagcgg
     2401 cagctccgcg acggcatacg agcggaagtc gacgagcgag aggtgacgga cgtgcatggc
     2461 cctgccgtca cctgcctcgg tcggatcagt gtgcgggcga tgccttgggg gcggcccggc
     2521 gcgtggtggt cttcttggca gcgaccttag ccgcagccgg tgcctgaccc ggtgacgcct
     2581 tggctccggc gctctcgggc gcggtggcgc gcaggttctc gccctcagcc accgtcatca
     2641 ccgcgtgccc accgaactgc ccgcgcaacg cagccacggc ttgcaaggcc ggcgacaccg
     2701 gctggcgcga ggcgaaccgg gcgaagagcg acgcggcgat gaccggcatc gggacggcgt
     2761 tgtcgatcgc ctcctcgacg gtccagcgac cttcgccgga gtcgttggtg tagtccgaga
     2821 cgtcggccag cgtcgggttg tcctcgaggg cgtcgacgag caggtccagc agccaggacc
     2881 gcacgaccgt ccctcggctc caggccttaa agcagccggg cacgtcggtg acgatgtcct
     2941 tcttggccag cagctcgtag ccctcgccat aggcgtgcat catggcgtac tcgatgccgt
     3001 tgtggaccat cttggcgtag tgccccgcgc cgacttcgcc cgcgtggaca aaccccaggt
     3061 cgcgctcgcc ctcgggccgc agcgcgtcga agatcggcat cgcgaggttg atccacttct
     3121 tgtcgccgcc agccatcagg ccgtagccgt tctccagccc ccacacgcca ccggagacgc
     3181 cacagtcgac gtaaccgatc cccttctgcg ccaacagttt tgcgttggcg aagtcgtcgg
     3241 tgaagcggga gttcccgccg tcgatgacca ggtcaccctt ctcgagcagc ccggccagct
     3301 tggcaaccgt gtccctcgtc ggcgcgcccg cgggcaccat gatccacacg acccggggac
     3361 cgctcagctt cttgaccatc gtggccaggc tggcgacgtc gcgcacggcc gggttcttgt
     3421 cgtagccgat cacctcgtgg ccgcccgccc ggatgcgctg gcgcatgttg gcgcccatcc
     3481 ggcccagacc gatgagaccg agttgcatga cgggtgcctc ctgaagtgac ggtcgatagg
     3541 gacgctgcgg aggtcaggct atgccccgct caccagtggc tcaggtatgc cgcgtgccag
     3601 gcgcgcgctg cgacggctgt gaagtcgccc atgaagccgt ccctgatggt gttatcacac
     3661 cacctgctgc ggcttcatga gcggctccat gcccgccctc ccacccgtga agcctcagct
     3721 ggcgaagcgg acgggcatga ggacgtagcg gtaggacgtg tcggcctcac ccgtcgcctc
     3781 ccgctgccca gccatgaccg cggggcggct gggctgggtg aaggagaagc gggtgaaggg
     3841 ggtgccgatg acgccgagac cgtcgagcag gaagtgcggg ttgaacgcga tctcgatgtc
     3901 aggcccggtg agcgtcgact cgaccgcttc ggaggcctgc gcgtcgtcac cggtgccggc
     3961 ctcgatggcc acctgaccat cggtgaaccg caaccgcacg ggcgtgttgc gctcggcgac
     4021 gagggccacg cgcttgaccg cctcgatgag agcgccggtc tcgagcacgg cctcggagtc
     4081 gaccgaggtc gggaagatcg aggtgacctt ggggtattcg ccgtcgagca accgagtcgt
     4141 cgcgcgccgc tgccccgcct cgaaaccgat cagcccgtcg ccaccggcgt tgcctccgag
     4201 ggccaactcg accgagccgg cggcaccgag ggccttcgcg gtgtcactga gggtgcgggc
     4261 cgggatgagc gcggtgtatg ccgcgtcgga ccgggccggt gcccaggtga gctcgcgcat
     4321 ggccaggcgg tagcggtcgg tcgccagcag ggtgaccttg tcgccctcga tctgcatccg
     4381 gacgccggtg aggatcggca gtgtgtcacc ccggtcggcc gcgatggcga cctggtgcac
     4441 ggcttgagtg aacacgtcgc cggcgatggt gccggagggc tgcggcgcgg ccggcagggt
     4501 ggggtagtcg tcggcctgca tcatcagcag gctgaaccgg ctcgtcccgc aggtgaccgt
     4561 gaccttgttg ccctcggtcg agacgtcaac cgggcgagcg gggagggcgc gcgagatctc
     4621 cgagagcagg cgcccggtga ccagcacggt gccgccttcg tcaacgtcgg cggcgacggt
     4681 gaccttggcg gacacctcgt agtcgaaggc tgacagcgtg accgtgccgg cccgatcggc
     4741 ttcgatcagc acgccggaca ggacgggcac cggaggtcgg ttcggcagac cgcgagccac
     4801 ccaggccacg gcatcagcga gcacctcgcg ctcaacgcgg aacttcacga tcgtctccac
     4861 cctcgtgctt cttcgatcca gaccccggct caaccggcag aacggcgcgg gcgcgcgcac
     4921 caccgccgtg tcggggggaa ctccgaccct agtcggttgg gcgggaactg caagagggaa
     4981 gggtcaccgc agttgcgcac gctcctgcct gatccccagc gttgtcgggg cattggagag
     5041 aattggtgga ctcgtcgtcg tcataggtgt tgtggattct gtggataacc ctcaagtccg
     5101 caggtcagcg gcccaagcgg cctgggtatg ccgcgtgcat gagtcggtgg gtaactcacc
     5161 gagcctgttg acgggcacca cggacccaca gccagtgcac tgtgagggtg ttgttgtccc
     5221 ccaaatggtg gacgttgtcc acagctgtcc acagccgagt gtggacaact taggggaggg
     5281 ctcagggcag aggaagctgc agggggggag cgtgaggcgg gaggagcgtg acgcgacgga
     5341 gcgtgccgaa gtgaatgggt cagcgcgagt ggttgcggat gcgcgcggtg agctcggtga
     5401 cctggttgta gatcgcccga cgttcactca tcagctcgcg gatcttgcga tcggcgtgca
     5461 tgacggtcgt gtggtcgcgg ccgccgaagg cctggccgat cttgggcagc gacagctcgg
     5521 tcagctcgcg gcacagatac atggcgatct gccgggccgt caccaactgc cgcgagcgcg
     5581 aagaactgca caggtcatcg acggtgaggg cgtaatagct cgccgtctcg gcctggatgg
     5641 tcccgatggt gatctgcacc ggctggtcgg tggggacgag gtccttgagg accacctcgg
     5701 cgagggtgaa gtcgaccggc tgccggttga ggctggcgaa cgcagtgacc cggatgaggg
     5761 ccccttcgag ctcgcggatg ttcgaggtga tccgggtggc gatgtattcc agcacctcgt
     5821 ccggggcgct catccgctcc tggagggtct ttttgcgcag gatcgcgatg cgggtctcga
     5881 ggtcgggtgc ctgcacgtcg gtgatgaggc cccactcgaa ccgactacgc agccgctcct
     5941 cgaaaccact gagttgcttg ggcggcaggt cactggtgat gacgacctgc ttgctgttgt
     6001 tgtgcagcgc attgaaggtg tggaagaact cctcctgcgt ctgcaccttg ccggcgagga
     6061 actggatgtc gtcgatgagc agcacgtcga catcgcggta gaggctctgg aaggtgctcg
     6121 cccggtcgtc acggatcgag ttgatgaact cgttcgtgaa ctcctcggag ttgacgtagc
     6181 gcacccggaa gccgggatag atctggcgga cgtagtggcc gattgcgtgc agcaggtggg
     6241 tcttgcccag ccccgaggag ccgtagatga acaacgggtt gtaggccttg gccggcgcct
     6301 cggacacggc gttggccgcg gcatgcgcga accggttgct cgacccggcg acgaaggtgt
     6361 cgaaggtgta cttcgggttg agccggctgc ggtcctggtc accgtcgcgt cgggggaagg
     6421 cgatcacccc aggggtatgc cgcccgctcc cctcaggatg gaagcgttcc atccgcgcgg
     6481 cgtcggggct ctcgtggcgc tcgtcgccgt tggcagtgtc gaacccgttg gcggggtcga
     6541 ccccgttgga gctgggggtg gcgtgctcga gggtggggtc gaccgtgacg gccagcccga
     6601 tgtcgcggcc gagcgctccg ctgagcgtcg cgacgaggtg ctcgcggacg tccgactcga
     6661 gcaccttctt ggtgtagtcg ttgggcacgg cgatgagggc ggtctgttcg acgacggcca
     6721 tcaggcgggc gaaaccgacg aaggcacgct cactggggga gacaccggtg cgttccagag
     6781 ccacgagggt gcgttgccac aggtcggtga gatcgacgtc tccgcctgtc acgtgtggct
     6841 cccctccttg ccgacgccgg gcccgatgga tcgggcctcc agacgttcga cattacccgt
     6901 catccacagc tctatccaca gggtggggac aaaccaccga tgggggaagg cctcgcagcc
     6961 ggaagcatcg tctgtccggg ggctgcgtgg agagattaca ccccttgatg ggcgtgtcgc
     7021 tcccctaaac cggaatgacg aggggggaga atgctgggtt ttcgttgctg gtcgaggtcc
     7081 cgggtttgac cctgcccgcc gacgggccgt accctgatct gcggcccatg tgtcgactct
     7141 gactccggcc gctttcgcgc gcccgtgctt gtcaccgcgg gcttccgatt gcggccggcg
     7201 gacggggtga cgcggcatac gggcgccccg cagtccaccg cagtccgccc gcaggctacc
     7261 cgccctgggc ctgccgatcg acggagacag acccgtgagc aagcgcacct tccagccgaa
     7321 caaccgccgt cgcgccaaga cgcacggctt ccgcctgcgg atgcggacgc gcgccggtcg
     7381 cgcgatcctc gccgctcgcc gtcgcaaggg ccgctccgag ctgtccgcct gaggcggata
     7441 tgtgctcccg gcccgcaatc ggctgcgtga gtcgggccag ttccgtgccg tcctccgcgg
     7501 ccctggtggg tcgcgagccg gcggaccgtt gatggtggtt cacgcagcga tgaccgatcc
     7561 gatgggtgct cgcgcccccc gggtcggttt cgtcgtgtcc ggcgccgtcg gaggcgccgt
     7621 cgtgcgtaat cgggtcaagc gccggttgcg cgcgctggcc gggactgcgc tgggcggcat
     7681 accggccggg tgtgacctgg tcgtgcgggc ggctccggcc gcggcgacag cgtcgttcgc
     7741 cgagttgcag gccgagttgg atcggtgcct gcgtcgcgcc ctggccaagg tggcgtgagc
     7801 tgatgacgct ggcagagctg ctcgggcccg gattgcgcgg tccgtggacg tggcgcaagg
     7861 cggctgcggc gcccctgctc atgctcgttc ggatttacca actggcgatc tcgccgctgt
     7921 tgccgcaacg gtgccgcttt tacccctcct gctcggcgta tgccgtcacc gccctcacgc
     7981 ggttcggtcc gctgcgcggt ggctggctgg ctgcgcgccg gctgggccgc tgccacccgt
     8041 ggaaccctgg cggcgttgac cacgttcccg agcgggaagc ctgccgcact cacgcggcga
     8101 cctagaccac ctggtccgcg agcggcatac ctggactgct gaccggaccg aaccatccgg
     8161 cgagatgaga gtctcgcctt tcgagcaagg acgacagtgg gcgacttcat caacctcatc
     8221 ctctcgccgc tgaccatcgg cgaggcctgg gtgatgcttg cctggcacaa ggtctggacg
     8281 acgatcggca tcggtggtgg ctgggcctgg gccttgtcga tcgtcgggct gacggtcgtc
     8341 gtgcgaatcg tgctcatccc gctcttcgtc aagcagatcc actcgtcccg gcggatgcag
     8401 ttgatccagc cggagatgca gaagatccag gcgaagtaca agggcaagac cgatcccgag
     8461 tctcggcagg cgatgactca ggagacgatg gatctctaca agaagaccgg gaccaacccg
     8521 ttctcctcgt gtctgccgat cctgctgcag tcgccgttct tcttcgcgct gttcacggtg
     8581 ctcaacaacc tcaagggcat cgcggagggg acccacccgg cgattgggcc gatcacgcca
     8641 gcggtggccc atgagatcga gtcgtcgaac atcttcgggg cgccgttctc ctcgaccttt
     8701 atggggtcga ccgacacggc cacgaagatc gtcacggtcg tcctcatcat cctcatgtcg
     8761 gtgtcgacgt tcacgacgca gcggcagttg atgcgcaaga acctgcccga ctcggcgctg
     8821 gacaacccgt tcatgaagca gcagacggtg ttgctctacc tcatgccgat cttcttcgcc
     8881 ctctcgggca tcaacttccc gatcggcgtt ctcctctact ggctgacgac caacgtgtgg
     8941 tcgatgggcc agcagttcta cgtcatccgc cggatgccgg cgccgggctc ggccgccgag
     9001 cgggcgctgc acgaacgcca ggagcgcaag ggcaagaccg tgaccccctt ctcgatcagg
     9061 gggctggagc gcgacgccgc cgcaggggcc gcagctgacg agacggttca ggacgcaccc
     9121 aacacggtta accgccagcg acagcaaccc aagagcaaga agcgggccaa gcgttccggc
     9181 ccggccaccg gcgcagccgg caccgacgac gccgagggat ccgcggcaga cggcaagcca
     9241 acagaccagt gaggtatgcc gtgccagcgc ggcatacgcg cggcggcggc tgacgaagga
     9301 gaagtgtcaa tgagcgaagc agggcaggac gtgacggagc tcgtcgaggc gagcgaaccg
     9361 gtggccgagg tcgacgagac cgtggttgac gagaccgtgg tcaacgaggt cgagcttgac
     9421 gaggtcgagg tgcagggtga gtccgactcg ggcgacctgc cggtcggcga tgagtctgat
     9481 gatgactccg acgacgactc ggacgacgac tcggacgacg acgaagagtc ggacgatgac
     9541 tccgatgagg agtcaggcga tgaggatgac gccgacggtg gcgacgacga ggccagcggt
     9601 ccgtccgcgg g
//
LOCUS       DAOMZY010000108         9586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_6748_length_9586_cov_6.208583, whole genome shotgun sequence.
ACCESSION   DAOMZY010000108 DAOMZY010000000
VERSION     DAOMZY010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9586
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6748_length_9586_cov_6.208583"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PLK64_07620"
     CDS             <1..356
                     /locus_tag="PLK64_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HPV79651.1"
                     /translation="SVCRGCGTELTTAATRKVGRCEDCPPTYDEATFEALRVWRGEVA
                     KTMKVPAYVVFTDATLTALAETAPTDLRGVAGVSGVGAMKLDRYGGAVLDVLAGGDPH
                     MAAEKTTAAMGSDNS"
     gene            500..748
                     /locus_tag="PLK64_07625"
     CDS             500..748
                     /locus_tag="PLK64_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79652.1"
                     /translation="MEINTMITRTTARLGDSGIGSDHLLLPTVPAVQGGTLPTPRRVV
                     IAGPAAIASNGEGVAFAAAYAASPKTDVHRSATGSPPV"
     gene            989..1276
                     /locus_tag="PLK64_07630"
     CDS             989..1276
                     /locus_tag="PLK64_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HPV79653.1"
                     /translation="MQLSTLIDTQTAAMSVGVGIPCRDYDAELWFAERPADVEFAKSL
                     CGTCPARTACLAGALERGEPWGVWGGQLLVQGTIVARKRPRGRPRKNDVAA"
     gene            1400..1540
                     /locus_tag="PLK64_07635"
     CDS             1400..1540
                     /locus_tag="PLK64_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79654.1"
                     /translation="MYQPVHEQLARVRHAEREARTGQVGIRRSARLISIQIRRQRRAP
                     HA"
     gene            1654..2232
                     /locus_tag="PLK64_07640"
     CDS             1654..2232
                     /locus_tag="PLK64_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HPV79655.1"
                     /translation="MQANLTIAQDPEADALLSSDPFALVTGMLLDQQFPMERAFAGPA
                     KLLARFGTLDPARIAEADPAAFADLAATPPAIHRYGRAMAGRIQQVAAHVVEHYDGDA
                     EAIWTTAKGPDVLIRRLVALPGFGEQKARIFAALLGKQLGVRPRGWREAIGPYAAPGT
                     FRSVADVVDAESLAKVRAFKQAAKAAAKASAQ"
     gene            2229..3683
                     /locus_tag="PLK64_07645"
     CDS             2229..3683
                     /locus_tag="PLK64_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009760593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79656.1"
                     /translation="MSEAPALGVVFPVEGDGRSTSATGRAVIADALEVVDPVGARAAR
                     DEANWRTGYPLHFRRLVEAGLGTTADAVGIADGGLTSVHSRMRWSRPEGTEVALGEAL
                     SGDLRPLETDRLAGSGEPEAAFSLPYRGARLSGDAAVAQVHDWVSRGIIEPSCGDAVE
                     QVIAHPEWLDLSDFTFVVLGAAAEMGPLRALLRWGAEVVAVDLARPDLWRGLLSDTRR
                     FGGTLRIPVAAGSAPVEERAGVDLVTEWPSLSAWLVEQPGTLVLGNYVYADGADNVRV
                     SVAVDALSMNLRAQRPDTALAFLATPTDVFAVPPADVERATAAYANARVSKALRGPLR
                     GLSGGRLLSRNYAPQANPGIADAIVPQQGPNYLLAKRIQRWRATTARAEGATVSMTVA
                     PPTRTRSVVKNRALAAAYAGAHRFGIEVFDPATANTLMAAVLVHDLRTGTKTLGHPWL
                     DEAHQAAHGGLWTAAYSARSALGLAAVLGLGSRS"
     gene            3680..3868
                     /locus_tag="PLK64_07650"
     CDS             3680..3868
                     /locus_tag="PLK64_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79657.1"
                     /translation="MTVAEDRIVCISCGSMPSPDDEGTARVEWVRGVENGRPVWTCPE
                     CSRRNLRSMEGKLDSAWW"
     gene            complement(3939..4583)
                     /locus_tag="PLK64_07655"
     CDS             complement(3939..4583)
                     /locus_tag="PLK64_07655"
                     /inference="COORDINATES: protein motif:HMM:NF025791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUKH-4 family immunity protein"
                     /protein_id="HPV79658.1"
                     /translation="MGMPPTSGTADRAGPDRIRLTPDEAAALDLPPTLHDWLTTTGLP
                     VLDGETLGVTFQHPCRVTPTPSAASTKRWPRLLDIGYEQWEPEHLRVGIDLDTLAVVG
                     YGPEPADNAFINSSVQTFITFLEHYRPVSDALKPAESSFRIIGPEEAAEMIRQLHEGT
                     LKPRPKPKAEPKPRINRKKAIDELRDTFKAADPDALKRNTYWPRIISQLRDGLI"
     gene            complement(4724..6052)
                     /locus_tag="PLK64_07660"
     CDS             complement(4724..6052)
                     /locus_tag="PLK64_07660"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HPV79659.1"
                     /translation="MTDLPRAAVTRAAKLATLPLGYAGRAALGLGKRVGGKPAEAVAA
                     EMQARAATQLFTVLGELKGGAMKFGQALSIFEAAWPEEAAAPYRAMLTKLQDSAPAMP
                     PARVKEVMAAEFGPRWRRRFLDFDETAVAAASIGQVHRATWRDGRDVAVKIQYPGAGK
                     ALLSDLSNVSKVARLATSWIPGIDIGPILEELRGRMSEELDYRLEASAHKAFAKGFEG
                     DPNVAVPGVIDGTERVLVTEWMDGIPLSSIIADGTQEQRDLAGTHYIEFLLDAPGRCG
                     LLHADPHPGNFRLLEDGRLGVLDFGAVKHLPGGLPDDMGRLLTLALDDDAGATLAGLR
                     EIGFVRDGIDVDAQRLLDYLQPFIDPLRTDTFHFSRDWIRDVFAHINDPRKPNYAVGM
                     RLNLPAEYLLIHRVWLGGIGVLCQLGATVPGRAKVAAHIPGADLPPVDEE"
     gene            complement(6057..7109)
                     /locus_tag="PLK64_07665"
     CDS             complement(6057..7109)
                     /locus_tag="PLK64_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79660.1"
                     /translation="MSQRGPRPDRSARASQPRLHPRRPPLRRGVDEVQLGVGPQALVL
                     RGLSEGQVAVLERLDGSRSLAQLHDVAADFGDPPHVVDELLTLLGRLGAMADPRSERV
                     DLLAARLGSEPRVLVGGPNALTDRLARALRQAGLEGVRVGPLALDQAELALRDREHTV
                     AGRQRPPDLVLLSSFDGLDPAEAAPWARHGIAHLAFTTEGDRVVVGPLVVPPPRGDTT
                     CIQCLELHRCDRDSGRASVLAQTSRPPAALADPRQWTDGLTALLGPGGPVRPTPMEPA
                     LLSAAAAVAALVVAAYLGGDCLPDGVTVELTSPWPRLDHRKWPRHPQCPHHRRAVAPT
                     PTIGAVVGSAAAAGRQ"
     gene            complement(7273..7437)
                     /locus_tag="PLK64_07670"
     CDS             complement(7273..7437)
                     /locus_tag="PLK64_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HPV79661.1"
                     /translation="MANDETYAGEFYCVKCKEKRTAEGKVVVSENGRRMAKAKCPVCG
                     TNLNRILGKA"
     gene            7669..8286
                     /locus_tag="PLK64_07675"
     CDS             7669..8286
                     /locus_tag="PLK64_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPV79662.1"
                     /translation="MDLDVALPFEVDAGHSPSPEDTPAVPTRGRRATSQEQPLVEVRR
                     STRRRRTVSAYRDGDRVVVLVPARFTRAEETRWVQTMTERLAASEAKRRPSDTDLAAR
                     AEALSERFLDGRARPASIRWVANQRGRWGSCTPSERTIRISSRVQGMPGYVLDYVILH
                     ELGHLLVAGHGPDFWAVLSSYPRLERARGYLDGVAAASGLALSDD"
     gene            8294..9055
                     /locus_tag="PLK64_07680"
     CDS             8294..9055
                     /locus_tag="PLK64_07680"
                     /inference="COORDINATES: protein motif:HMM:NF025615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HPV79663.1"
                     /translation="MARTAASSPARPGRRRRSPGWVPTQHGAWAMLVSPLLLGALASG
                     PGWIHLLLAAFWFAGYFAFNATSLWLKSGRKVRYLRPVQVYAALAAALGLATALADPG
                     LLRWAPLFVLPLGVGVWAAAHRRERDLLAGVTTVIGSALMTVVAYDAGGGPDLRRAAV
                     LAAGQFLYFAGTVFYVKSMIRERDNPAYLRLSVGFHVVAALALAPWAPWVAALFVVLA
                     LRAWWVPRLGWTPKQIGIAEIAGTTAVALVSLFTV"
     gene            9102..>9586
                     /locus_tag="PLK64_07685"
     CDS             9102..>9586
                     /locus_tag="PLK64_07685"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPV79664.1"
                     /translation="MPQLRSRTTTHGRNMAGARALWRATGLTDDDFGKPMVAIANSYT
                     QFVPGHVHLKDLGDLVAGAVREAGGVAREFNTIAVDDGIAMGHHGMLYSLPSREIIAD
                     SVEYMVNAHAADALVCISNCDKITPGMLLAALRLNIPTVFVSGGPMEAGTAVIGEDGT
                     AT"
BASE COUNT         1414 a         3288 c         3385 g         1499 t
ORIGIN      
        1 cttcggtatg ccgcggctgc ggcaccgagc tgaccaccgc ggccacccgc aaggtcggcc
       61 ggtgcgagga ctgcccaccg acctacgacg aggcgacctt cgaggccttg cgggtgtggc
      121 ggggcgaggt ggccaagacc atgaaggtgc cggcctacgt cgtcttcacc gacgccacgc
      181 tcaccgcgct cgccgagacc gcgcccaccg acctgcgcgg ggtcgccggg gtctccggcg
      241 tcggggccat gaaactcgac cgctacggcg gtgccgtgct cgacgtgttg gccggcggcg
      301 atccccatat ggctgccgaa aagactactg cggcaatggg ttccgacaac tcgtgacggt
      361 cgctccgaat gcgtccaaac ttttctcgtt aattcgtttg tccccggccg ccgccgaggg
      421 ttaccctcgt gaacgcacaa cctgcggggc ccgaagggcc caggcaggac gacgagagca
      481 aggaaggagg gcggctttga tggagatcaa caccatgatc acgcgcacga cggcgcgact
      541 gggcgacagc ggaatcgggt ctgaccacct cctgctgccc accgtcccgg ccgttcaagg
      601 gggcactctg cccaccccgc gtcgcgttgt catcgccggc ccggccgcta tcgccagcaa
      661 tggcgagggt gtggcctttg ccgccgctta cgccgccagc cccaagaccg atgtccaccg
      721 ttccgccacc gggagcccac ccgtctaggc ccacctagac cggactcacc tccaggccgc
      781 ggaacccgac aagggatccg cggccttcgt gtttgtcgcg ggggccgacg ccccgaaggc
      841 cactgagatg aacaccctga gatcgcgcca gaccgccaga ccgcaggacg aacaacatgc
      901 ccgggtccct caccagggat cgcccggaca acaggacaga acggcccccg ccaccgcgga
      961 cggccgacag gaggaggagg tgacaccgat gcagctgagc accctcatcg acacccagac
     1021 ggcagcgatg tccgtcggcg tcggcatccc gtgccgtgac tacgacgcgg agctctggtt
     1081 cgcggagcgc ccggcggacg tcgagttcgc caagtcgctg tgcggcacct gcccggcgcg
     1141 caccgcgtgc ctcgccgggg cccttgagcg gggcgagccg tggggcgtct ggggcgggca
     1201 attgctcgtc caaggcacca tcgtcgcccg caagcgtcct cggggccgtc cgcgcaagaa
     1261 cgacgtcgcg gcgtgaggca gggggcgggc gctcccgatc gcatcccggg agcgcccgca
     1321 aggcgcccga ccgtcctctc gctcgtccca cccgctccac cagtcatacc cagacctcgt
     1381 cctgaagggg accaccgcca tgtatcaacc agtccacgag cagctggctc gtgtgcgaca
     1441 tgccgaacgc gaggcccgaa ccggccaggt cggcatccgg cgcagcgccc ggctgatctc
     1501 catccagatc cggcgccaac gccgggcacc gcacgcctga tccacccaac tgtcggtgga
     1561 gtgttggcca catcgcagcg tggccaacac tccaccggcc tgcttctccg gcgaaacgac
     1621 ctggcggcgg gcacgcggca tacagtgacc tccgtgcagg ccaacttgac catcgcccag
     1681 gaccccgagg ccgacgcgct tctctcgtcg gacccctttg ccctagtgac ggggatgctg
     1741 ctcgatcagc aattccctat ggaaagggcg ttcgccggac cggccaaact gctggctcgg
     1801 ttcggcacgc ttgaccccgc gcgcatcgcc gaggctgacc cggctgcttt cgccgacctg
     1861 gccgccaccc cgccggcgat ccaccggtac ggccgggcca tggccggacg gatccagcag
     1921 gtggccgcac atgtggtcga gcactacgac ggggacgccg aggccatctg gaccacggcc
     1981 aaaggccccg acgtgctcat ccgtcgcctc gtcgccctcc ccggcttcgg ggagcagaag
     2041 gcccgcatct tcgctgccct gctgggcaaa caattgggcg tgcgcccccg cgggtggcgc
     2101 gaggcgatcg gcccgtatgc cgcacccggc accttccgct cggtggcgga cgtcgtggac
     2161 gccgagtccc tggccaaggt gcgagcgttc aagcaggcgg ccaaggcggc agccaaggcg
     2221 agtgcccaat gagcgaggcg ccggctcttg gcgtggtctt ccctgtcgag ggcgacggcc
     2281 gaagcacgag tgccaccggg cgtgccgtca tcgcggatgc gcttgaggtc gtcgacccgg
     2341 tgggagcgcg ggcggctcgt gacgaagcca actggcgcac cggctatcca ctgcacttcc
     2401 ggcgcctcgt tgaagccggg ctgggcacca ccgccgatgc ggtcggaatc gccgatggcg
     2461 gactgacttc cgtccacagc cggatgcgat ggagccgtcc agagggcacc gaggtcgctc
     2521 tgggcgaggc gctctcgggc gacctgcgac cgctggagac cgatcggctg gccgggtcgg
     2581 gtgagccgga ggcggccttc tccctgccgt atcgcggggc tcggctgtcc ggcgacgcgg
     2641 cggtcgccca ggtccacgac tgggtgtcgc gagggatcat cgagccgtcc tgcggcgacg
     2701 ccgtggagca ggtcatcgcc catcccgaat ggctcgacct ctcggacttc accttcgtcg
     2761 tgctgggcgc ggccgcggag atgggaccgc tgcgcgcgtt gctgcgctgg ggcgccgagg
     2821 tggtcgcggt cgatttggcc cgaccagacc tctggcgggg cctgttgtcc gacacccgcc
     2881 ggttcggtgg caccctgcgt atccccgtgg cggccgggtc cgcgcccgtc gaggagcgcg
     2941 ccggtgtcga tctggtcacc gagtggccca gcctgtctgc atggttggtg gagcagccgg
     3001 gcaccctcgt cctgggcaac tacgtctatg ccgacggcgc cgacaacgtg cgggtcagcg
     3061 tggccgtcga cgccctctcg atgaacctgc gcgcccagcg acccgacacg gcgcttgcct
     3121 tcctcgcgac tccgacggac gtgtttgctg tcccgccggc cgacgttgag cgggcgaccg
     3181 cggcatacgc gaatgcccgc gtgagcaaag cgctgcgggg gccgctgcgt gggttgtcgg
     3241 gtgggcggct gctcagccgc aactacgcac cgcaagccaa ccccgggatc gctgacgcga
     3301 tcgtgccgca gcagggcccc aattaccttc tcgccaaacg gatccagcga tggcgggcga
     3361 ctactgcacg cgcggagggc gcgaccgtgt cgatgaccgt cgccccgccg acgcggaccc
     3421 ggtcggtcgt caagaaccgg gccctggccg ctgcctacgc cggtgctcat cggttcggca
     3481 tcgaggtgtt cgacccggcg acggccaaca ccctgatggc ggctgtgctc gtccatgacc
     3541 tgcgcaccgg gacgaagacc ttgggtcatc cgtggttgga cgaggcgcac caagccgcac
     3601 acggcggtct gtggacggcg gcatactcgg cgcgaagtgc gttggggctg gcggccgtgc
     3661 tcggtctggg ctcccggtcg tgaccgtggc cgaggaccgg atcgtctgca tcagttgcgg
     3721 cagtatgccg tcccctgacg acgaggggac cgcacgggtc gagtgggtgc gcggcgtcga
     3781 gaacgggcgt ccggtgtgga cctgccccga atgcagccga cgcaacctgc gcagcatgga
     3841 aggcaaactc gactccgctt ggtggtgagc cgttgactga cccggaccgt cgccgaccga
     3901 cgtggacggc aagcggtcaa cccggcgtcg acccgcggtc agatcaggcc gtcgcgcagt
     3961 tgggagatga tccgtggcca gtaggtgttg cgtttcaacg catccgggtc cgcagccttg
     4021 aaggtgtccc gcaactcgtc gatggccttc ttgcggttga tccggggttt gggctcggcc
     4081 ttgggcttcg gccgcggctt cagggtgcct tcatgcaact ggcggatcat ttccgcggcc
     4141 tcctcagggc cgatgatccg gaacgagctc tccgcgggtt tgagggcgtc ggagacgggt
     4201 cggtagtgct cgaggaaggt gatgaaggtc tggacgctgg agttgatgaa ggcgttgtcg
     4261 gcgggttcgg ggccgtagcc gacgacggcg agggtgtcca ggtcgatgcc gacgcggagg
     4321 tgctcgggct cccattgttc gtacccgata tcgaggagcc gtggccaacg cttggtgctg
     4381 gcggcgctgg gggtgggggt gacgcggcag gggtgttgga aggtgacccc gagggtttcc
     4441 ccgtcgagaa cgggcaggcc ggtggtggtc agccagtcgt gcagggtggg aggcaggtcc
     4501 agggcggccg cctcgtcggg tgtcaggcgg atgcgatccg ggccggcgcg gtccgcggtc
     4561 ccggatgtgg gcggcatacc catgtcaggg tcgcctcgtt gcggtccggt ggtggccccc
     4621 cgagggcctg ttcggccgag tgctttgcat gggcgggcac gctatcaggg gccctgacgc
     4681 gaagccatgg cccgctcgtc gcgctgcggc ggctggagcc cgctcactcc tcatcgaccg
     4741 gcggcaggtc agcaccgggg atgtgggccg caaccttcgc tcgaccagga accgtcgcac
     4801 cgagttggca cagcacgccg atcccgccga gccagacccg gtggatgagg agatattcgg
     4861 cgggcaggtt gagccgcatc cccacggcgt agttgggttt gcgcgggtcg ttgatgtggg
     4921 caaagacatc ccggatccag tcgcgggaaa agtggaaagt gtccgtgcgt aagggatcga
     4981 tgaacggctg aaggtagtcc agcaggcgct gggcgtcgac atcgatcccg tcacggacga
     5041 acccgatctc gcgtaaaccc gcgagagtgg cccctgcgtc gtcgtccagg gccaacgtga
     5101 gcagacgtcc catgtcgtcg ggcaggccgc cgggcaggtg cttgacggcg ccgaagtcca
     5161 gcacccccag tcggccgtcc tcgagcagcc ggaagttgcc cgggtgcggg tcggcatgga
     5221 gcaagccgca acgcccagga gcgtcgagga ggaactcgat gtagtgcgtc ccggcgaggt
     5281 cacgctgttc ctgggtcccg tcggcgatga tgctcgacag gggtatgccg tccatccact
     5341 cggtcactag gacgcgctcg gtgccgtcga tgacaccggg gacggccacg ttcgggtcgc
     5401 cctcgaaacc cttggcgaag gccttgtggg ccgaggcttc gagccggtag tccaactcct
     5461 cggacatccg cccccgcaac tcctcgagga tcgggccgat gtcgatcccg gggatccacg
     5521 aggtggccaa ccgggccact ttggacacgt tagacaggtc ggacagcagc gcctttccgg
     5581 cccccgggta ttgaatcttc accgcgacgt cacgtccgtc acgccaagtg gcccgatgga
     5641 cctgaccgat cgaggcggcc gcgacggccg tttcgtcgaa gtcgaggaat ctccggcgcc
     5701 aacgcggacc gaattcggcg gccatcactt ccttgacccg agccggaggc atggctggtg
     5761 cgctgtcctg cagtttggtg agcatggccc ggtagggagc ggcagcctcc tcgggccacg
     5821 cggcctcgaa gatcgacagc gcctggccga atttcattgc gccacccttg agttcgccga
     5881 gcacggtgaa cagttgagtg gcggcccgag cctgcatctc agcggcaacg gcctcggccg
     5941 gcttgccccc gacccgtttg ccgagaccga gagccgcgcg accggcatac cccaggggga
     6001 gcgtcgcgag cttggcggcg cgggtcactg ccgccctggg gagatcggtc acgcgctcat
     6061 tgtctcccgg cggctgcggc gctgccgacc accgccccga tggtgggcgt cggcgcgaca
     6121 gccctccgat ggtgggggca ctgaggatgc cggggccact tgcggtggtc caaccgtggc
     6181 cacggggagg tgagctcgac cgtcacccca tcggggaggc agtccccgcc gaggtatgcc
     6241 gcgaccacca gcgccgcgac cgcggccgcc gcggacagca gggctggctc catcggggtg
     6301 gggcggaccg ggccccctgg gccgagtagt gccgtcagac cgtcggtcca ctgtcgtgga
     6361 tcggcaaggg ctgcgggcgg ccggcttgtc tgggccaaca cggatgcccg ccccgagtcg
     6421 cggtcgcagc ggtgcagttc caggcattgg atacacgtcg tgtccccgcg tggcggcggc
     6481 acgacgagtg gaccgacgac gacccggtcg ccctcggtcg tgaaggccag gtgagcgatg
     6541 ccatgcctag cccagggcgc cgcctccgcg gggtcgagtc cgtcaaagga actcagcagg
     6601 accagatcgg gtgggcgctg ccggcccgcg acggtgtgct cgcggtcgcg cagggcaagc
     6661 tcggcctgat ccagggccaa cggccccacc cgaaccccct cgaggcccgc ctgccgcagc
     6721 gcgcgggcca ggcgatcggt gagcgcgttt ggaccgccga caagcacccg aggttcgctc
     6781 ccgagtcgag ccgcgaggag atcgacgcgc tccgaccgtg gatcggccat cgcgcccagc
     6841 cgacccagca gggtgagcaa ctcgtcgacg acgtgtggtg ggtcaccgaa atcggctgcg
     6901 acatcatgaa gttgggcgag cgaccgggac ccgtccaggc gctcgagaac ggcgacctga
     6961 ccttccgaca gcccgcgcaa cacgagggct tgggggccga cccccaactg cacctcgtcg
     7021 acgccgcgac gcagcggagg gcgacgcggg tgcagcctgg gctggctggc acgcgccgag
     7081 cgatccggtc gggggcctcg ctgcgacatg atgcgaatct gccacagcat gggccaggtc
     7141 cgtcagcgtc atccacaggc ccgcgaatgc cggggtgtgc gtgccgggag gtaccggacg
     7201 ggtgatccgg tcagaatgca cggagggcaa cccgcgttgc gggtcgccct ccgtgtcctg
     7261 tcgagctgcg gatcaggcct tgcccaggat ccggttgagg ttggtgccgc agaccgggca
     7321 cttcgccttc gccatgcggc ggccgttctc gctgacaacg accttgccct cagcggtcct
     7381 cttctccttg cacttcacgc agtagaactc gcccgcgtag gtctcgtcgt tggccatgtg
     7441 tggtcctcct ggtcggggtg tggggctcgt gactccgccc atcggcgggt ggcatcccgg
     7501 acaaccctag accagcgaac cgggtggaac gtgaattgag cccttcccgc acgtcggccc
     7561 gtcgtgcctg ccgcaggcgg gctggttggc agcatggcca gaagtgtggg ccacagcccg
     7621 gccacgttca gccctcctct gtcggtggcg catcgtatcg tgggcggtat ggacctggac
     7681 gtggcgctgc cgttcgaggt cgatgctggg cactcgccat ccccggagga cactcctgct
     7741 gtgccgacca gagggcggcg ggcgacgagc caggagcagc cgctcgtcga ggtgcggcgc
     7801 agcacccgac gccgtcgcac cgtgagcgcc taccgcgatg gcgaccgggt cgtcgtcctc
     7861 gtccccgccc ggttcacccg ggccgaggag acgcgatggg tccagacgat gacggagcgg
     7921 ctggcggcca gcgaggccaa acgtcgtcca agcgacacgg atctggcggc ccgggccgag
     7981 gcgttgtccg agcggttcct cgacggtcgg gccaggcccg cttcgatccg gtgggtggca
     8041 aaccaacgcg gtcgctgggg gtcctgcaca ccgtccgagc gcacgatccg gatttcctct
     8101 cgcgtccaag gcatgcctgg ctacgtgttg gactacgtca tcctccacga gctcggccac
     8161 ctattggtcg ccggtcacgg gcccgacttc tgggccgtgc tgtcgagcta cccacggctc
     8221 gagcgggccc gtggttacct cgacggggtc gctgccgctt ccgggctggc cttgagcgac
     8281 gattgatccc gtgatggctc gcacggccgc ttcatcgccg gcccgccccg gtcgccgcag
     8341 acgttcgccc ggctgggttc cgacccaaca cggagcctgg gcgatgctcg tcagcccgtt
     8401 gctcctcggc gcccttgctt cgggcccggg ctggatccac ctgctgctgg cggcgttttg
     8461 gttcgcgggc tacttcgcct tcaacgccac gagcctctgg ctcaagtcgg gccgcaaggt
     8521 cagatacctg cgtccggtcc aggtgtatgc cgccctcgcc gctgctctcg ggctggccac
     8581 ggcgctggcc gatcccggct tgctgcgttg ggcgcccctc ttcgtgctgc ccttgggggt
     8641 cggcgtctgg gcagccgccc accgccggga gcgtgacctg ttggccgggg tcaccacggt
     8701 catcgggtcg gcgctcatga cggtcgtggc ctatgacgcg ggtggtggtc ctgatctgcg
     8761 ccgggccgcg gtgctggcgg ccgggcagtt cctctacttc gcgggcaccg tcttctacgt
     8821 caagtcgatg atccgcgaac gtgacaaccc ggcatacctg cggctgtcgg tgggcttcca
     8881 cgtggtcgcg gccctcgcgt tagcgccgtg ggctccatgg gtggcggcgc tgttcgtggt
     8941 gctggcgctg cgggcttggt gggtgccgcg cctggggtgg acgcccaaac agatcgggat
     9001 cgccgagatc gccgggacca ccgcggtcgc gctcgtgtcg ctcttcacgg tctgagcccg
     9061 cactaacctg gggactgaac acccgtcaag agaggtcgac catgccgcag ctgcgttccc
     9121 ggacaaccac ccacggtcgc aacatggccg gggccagggc cctgtggcgc gcgaccggcc
     9181 tcaccgacga cgacttcggc aaacccatgg tggcgatcgc caacagctat acgcagttcg
     9241 tcccgggaca tgtgcacctc aaggacctcg gtgacctcgt ggccggggcc gttcgtgagg
     9301 cggggggagt ggctcgggag ttcaacacga tcgccgtcga cgacggcatc gccatgggcc
     9361 accacgggat gctctacagc ctgcccagcc gcgagatcat cgccgactcg gtcgaataca
     9421 tggtcaacgc gcacgccgcc gatgcgctcg tctgcatcag caactgcgac aagatcacgc
     9481 cgggcatgct gctggcggcc ctgcggctga acatccccac cgtgttcgtc agcggtggcc
     9541 cgatggaggc cggcacggcg gtcatcggcg aggacggcac cgcgac
//
LOCUS       DAOMZY010000109         9583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_6753_length_9583_cov_5.475966, whole genome shotgun sequence.
ACCESSION   DAOMZY010000109 DAOMZY010000000
VERSION     DAOMZY010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9583
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6753_length_9583_cov_5.475966"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..172
                     /locus_tag="PLK64_07690"
     CDS             <1..172
                     /locus_tag="PLK64_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HPV79665.1"
                     /translation="FTYSITVGIGAGFIAFVFIKLVRGKSSAIHPLMWLVSGLFVLYF
                     AIDPLRRILGAG"
     gene            complement(268..1653)
                     /locus_tag="PLK64_07695"
     CDS             complement(268..1653)
                     /locus_tag="PLK64_07695"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HPV79666.1"
                     /translation="MTTGDPDPTASTGASPPLASPATRAEAASERRIRHRRRRLILTV
                     AAAIVVLIVGGVSGFLVVKASSMTSKVFAGGGGVAGLVGGGAPLATGPDGRVNILIFG
                     TSQDDAAHSTGSGGQGMWLTDSIQLLSINPVDHTAVMVAVPRDTWVKLGNKCVVGAAA
                     KVNAVYECATGVFDKPQAEVPDYQQTDATGARALMAAVNTVTGIQPQYWVHINYTVLR
                     TAVDAVGGIDVEIVGNGHEGIFDTNADGGCPHEEVACRAVYYPRDGIYRIDGTHALSL
                     ARARGDGNPRSCMQFGLNGGDFDRQANQQKITVALKNRAVSAGTLANPAAVSGLIDAL
                     GDNVTTNLSTDEAKTAISLAREMGDMTPISLVDPAHPVMTTGTVSGQSVVHPLAGTFD
                     YSAIHRFIDSRIHSIDPTSTATATATATSSGSATSAGPAASATPSTSAAADPTSSAKS
                     TYERPASCWER"
     gene            1771..3009
                     /locus_tag="PLK64_07700"
     CDS             1771..3009
                     /locus_tag="PLK64_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV79667.1"
                     /translation="MDRSATFASLRVHNYRRYAAGGLVSNAGTWMGRVAQDWLVLTEL
                     TDHSSTALGVATGLQFAPFLVLAPWTGMVADRFPKRRILVTTQTTLAVTALLLGILTV
                     SGVVQLWQVYALAFVQGLAQAIDNPARQAFVVEMVGPGHLANAVALNSASFNAGRLIG
                     PGVAGLIIAAYGTGVAMVVNSLSFVLLLVALLAMRADELQPAPLARGRGRIREGLVYV
                     RGRTDIKVILVLVFVLGTFGMNFQITTALMATQAFGKGPTEFGILGSIMAIGSLSAAL
                     LSARRARPRLRVLLVALAGFTVATGLAAVAPTYELFALALIPCGLAALTALTTANTLV
                     QLSVDPAMRGRVMALYMAIFLGGTPIGAPLIGRLGDWAGPRWTIGVGSVAVGLSVVIV
                     GGWLVKRENVDMLIRKRGRR"
     gene            complement(3313..4443)
                     /gene="serC"
                     /locus_tag="PLK64_07705"
     CDS             complement(3313..4443)
                     /gene="serC"
                     /locus_tag="PLK64_07705"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HPV79668.1"
                     /translation="MTDQPATLTIPTDLLPGDGRFGCGPSKVRPEQVARLSEIGGTIL
                     GTSHRQAPVKGLVGEIRSGLRDLFSLPEGYEIILGNGGSTAFWDIAAFGLVRAKAQHL
                     SFGEFSNKFASVTNTAPFLGESTIIKAAPGTLATAVAEAGADVYAWPHNETSTGVMAP
                     VQRVEGADADALVLIDATSGAGGLPVDMSQADVYYFAPQKCFASDGGLWLAAFSPAAL
                     ARVEEITASGRWVPDFYSLPTAIDNSLKNQTYNTPAVATLAMFAAQLDWMNGNGGLAF
                     TTGRTADSASRLYGWAERTAYTTPFVSDPGARSQVVGTIDFDDTVDAAAVAKVLRANG
                     IVDVEPYRKLGRNQLRIAMFPAIDPEDISALTASIDWVVGQL"
     gene            4695..5516
                     /locus_tag="PLK64_07710"
     CDS             4695..5516
                     /locus_tag="PLK64_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79669.1"
                     /translation="MHPHHHSSSADGDHPTDAEPTEGRRRGPGRRRGPDFGMGFGPAF
                     AMGAAGPDGPGMPFGPGGPGGPGGPGGPGGPGGRGRGPGGRGRGRGGPGGPGPFGRPG
                     GWQQADLPSADDVAEWFAGRLPADWFIGDAQISVDREEIVVVGELPPVDGQGDSAAAA
                     EGRISRFREQSRDERIAIATQAQERYGRKVSWGARIGERRELFTHLAVPVMTRLRQPE
                     RQVLDTLVDAGVARSRSEALAWAVALVGQHTETWLSDLRAKMAEVDKLRAQGPQL"
     gene            complement(5602..6744)
                     /locus_tag="PLK64_07715"
     CDS             complement(5602..6744)
                     /locus_tag="PLK64_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase 2"
                     /protein_id="HPV79670.1"
                     /translation="MTTQPLAAPEDRFKPGLEGIVACETEIAEPDKEGSALRYRGVDI
                     EDLVDKVSFGQVWGLLVDGAFEPGLPPAEPFPLPVHSGDVRVDVQSALAQLAPIWGFR
                     PLFDIDEEEARENLARAAVMALSFVGQSARGVHKPMVPQREVDKARTIVERFMIRWRG
                     EPDPRHVEAVDAYWVSAAEHGMNASTFTARVIASTGADVAACLSGAVGAMSGPLHGGA
                     PSRVLHMLEAVENLGDATAYVKGVLDRGERLMGFGHRVYRAEDPRARVLRATCKRLDA
                     PRYEAAHALEVAALAELRERRPDRVLETNVEFWAAVILDYAEVPPSMFTPMFTCARTA
                     GWSAHILEQKRAARLIRPAARYIGIHERRPEDVPGWPRHIDPHEVH"
     gene            complement(6849..7256)
                     /locus_tag="PLK64_07720"
     CDS             complement(6849..7256)
                     /locus_tag="PLK64_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79671.1"
                     /translation="MAKSTIVGNAVKYGVKYGPHVIVAAKALKEPATAYAQKKLAEQK
                     SRRAAAAEATTLQAGTVLRVFHQGQPVWVVYSGEEPIASHPVVEATLAQLVERADLSQ
                     RFVPEPAVTAKDRITSAGGQAAGKARAALKRTR"
     gene            7312..8016
                     /locus_tag="PLK64_07725"
     CDS             7312..8016
                     /locus_tag="PLK64_07725"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase"
                     /protein_id="HPV79672.1"
                     /translation="MTDRVDYTGEGLDEADLASTPYAQLTRWIGQAQAEGVRAAESPE
                     GLALQFATVDAAGAPNVRTVLMRFLDPRGPGFVTDAGSIKGREIAANPAVAASLGWPE
                     LFRVVRFRGLAEPIEPEALADYFSQRPWGSRVAAWASHQSEQVASRAALLAEFDRYAA
                     QFPDRGNPDDVPVPPSWSGFRIRATEVEFWAGRSSRLHDRIVFTAPPAAELGAGAGQV
                     PLLDDPSAWRVSRRQP"
     gene            complement(8268..8852)
                     /locus_tag="PLK64_07730"
     CDS             complement(8268..8852)
                     /locus_tag="PLK64_07730"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPV79673.1"
                     /translation="MARSSQTHLAVLGALSVEPMTGYAVRAAITQTLGHFWSESFGQI
                     YPTLTELIARGEIRPTEPGRTSGSRFELTAAGRTRLRELLAEPAAVSPPRNGLLLRLF
                     LGGQLGSAHCRVLVQESADRASAMLAGYATIRTETEAEIAAARAANDDTAAASAAYRL
                     LTLSAGEYAAHAHLAWAAETLATLATLPELAETP"
     gene            8925..9332
                     /locus_tag="PLK64_07735"
     CDS             8925..9332
                     /locus_tag="PLK64_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017197367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79674.1"
                     /translation="MASDAVTPVTPTWPRKAAAATYAGVAGFQVALAAGAPWGVAAWG
                     GAHEGVLPMGFRVGSVVSAGVWGLVAASLGTSWPPARWRRPVLTGLAVLAPVSFAMNL
                     ATPSPVERAIWAPITAAQTVLVLLAFRAERRAR"
     gene            9393..>9583
                     /locus_tag="PLK64_07740"
     CDS             9393..>9583
                     /locus_tag="PLK64_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV79675.1"
                     /translation="MSSTPQVTSLRPIAVAAYGPTVLSSIGTGAVLPVLALTARDLGA
                     SVGTAAVIVSLLGVGMLVG"
BASE COUNT         1295 a         3189 c         3566 g         1533 t
ORIGIN      
        1 gttcacctac tcgatcacgg tgggcatcgg cgccggcttc atcgcgttcg tcttcatcaa
       61 gttggtccgc ggcaagtcct cggcaatcca cccgctcatg tggttggtct cggggctgtt
      121 cgtcctctac ttcgccatcg acccgctgcg ccggatcctc ggcgccggct gaccgaccgg
      181 agccgccaac caccaaccag caccaccacc gacggccggt atgccgcggg cgcgagccac
      241 gcggcatacc ggccgtttcg ttgtggttca gcgctcccag caggaggcgg ggcgctcgta
      301 ggtcgacttg gctgaggatg tcgggtccgc ggcggcagat gtggacggcg tcgcagatgc
      361 cgcaggtccc gccgaggtcg ccgaccctga ggacgtcgcg gtggccgtgg ccgtggccgt
      421 cgaggtcggg tcgatcgagt ggatccgcga gtcgatgaat cggtggatcg ccgagtagtc
      481 gaacgtgccc gcaaggggat ggacgacgct ctggccggag accgtgccgg tggtcatcac
      541 gggatgcgcc gggtcgacga gcgagatcgg cgtcatgtcg cccatctccc gggccaagga
      601 gatcgccgtc ttcgcttcgt cggtgctgag gttggtggtc acgttgtcgc cgagggcgtc
      661 gatgagcccg ctgacggcgg cggggttggc cagagtgccg gccgagaccg cgcggttctt
      721 gagcgcgacg gtgatcttct gttggttggc ctggcgatcg aagtcgccgc cgttgagccc
      781 gaactgcatg caactgcgcg gattgccgtc cccacgggcc cgagccaggc tcaaggcgtg
      841 ggtcccgtcg atgcgataga tcccgtcgcg ggggtagtag accgcccggc aggcgacctc
      901 ctcatggggg cagccaccgt cggcgttggt gtcgaagatc ccctcgtgcc cgttgccgac
      961 gatctccacg tcgatgccgc ccacggcgtc gaccgcagtc cgcagcacgg tgtagttgat
     1021 gtgaacccag tactgcggct ggatcccggt gaccgtgttc acggcggcca tcaaggcccg
     1081 cgcgccggtc gcgtcggtct gttgatagtc aggcacctcg gcctggggct tgtcgaacac
     1141 tcccgtcgcg cattcgtaga ccgcgttgac cttcgcggca gcgccgacga cgcacttgtt
     1201 gccgagtttg acccacgtgt cccgcggcac ggccaccatg accgcggtgt ggtcgacggg
     1261 gttgatgctc agcagttgga tcgagtcggt gagccacatg ccctggccgc cggacccggt
     1321 ggagtgggcg gcatcatcct gggaggtgcc gaagatgagg atgttgaccc ggccgtccgg
     1381 gccggtcgcg aggggcgcgc ccccgcctac gagtccggcc actccgccgc ccccggcgaa
     1441 taccttggag gtcatcgacg acgccttgac gacgaggaat ccgctcacgc caccgacgat
     1501 caagacgacg atggcggctg cgacggtgag gatcaggcga cgtcggcgat gcctgatgcg
     1561 acgttcggac gccgcttctg cccgcgtggc gggggaggcc aagggcggtg acgcgccggt
     1621 ggacgcggtc gggtcggggt caccggttgt catggcctca agtcaatgga atcgtcgaca
     1681 gttccgttcc agggtacgcg ccggatctgt cggtcgtaga cgcagagcgc gacggccggc
     1741 tcggtcccgc agccgtgcga ggatgccgag gtggaccgca gcgcgacctt cgcctcgctg
     1801 cgggtccaca actatcgccg gtatgccgcg ggcggactgg tcagcaacgc cggcacgtgg
     1861 atgggtcggg ttgcgcagga ctggctggtc ctcaccgagt tgaccgacca ctcctcgacc
     1921 gcattgggtg tggcgaccgg gctgcagttc gcgcctttcc tcgtgctcgc accgtggacg
     1981 ggcatggtcg ccgatcgctt ccccaagcgc cggatcctcg tgacgaccca gaccacgctg
     2041 gcggtgacgg ccctgctctt gggcatcctc acggtgtccg gggtcgtcca gctctggcag
     2101 gtgtatgccc tggcgttcgt ccagggtctg gcccaggcca tcgacaaccc ggcccggcag
     2161 gcgttcgtcg tcgagatggt cgggccgggg catctggcca atgccgtcgc tctcaacagc
     2221 gcgtcgttca acgccgggcg gctcatcggc ccgggggtcg cgggactgat catcgcggca
     2281 tacgggacgg gggtggcgat ggtcgtgaac tcgctgtcct tcgtcctgct cctggtcgcc
     2341 ttgttggcga tgcgggccga cgagcttcaa cctgcgccgc tcgcgcgcgg gagggggcgg
     2401 atccgcgaag ggctggtcta tgtccgcggg cgcaccgaca tcaaggtcat cctggtgctc
     2461 gttttcgtcc tggggacgtt cgggatgaac ttccagatca ccaccgcgct tatggcgacg
     2521 caggcgtttg ggaaggggcc gaccgaattc gggatcctcg gctcgatcat ggcgatcgga
     2581 tcgctgtcag cggcgctgtt gtcggctcgt cgggcgcgtc cacggctgcg ggtcctgctc
     2641 gtcgcgctgg ccggtttcac ggtggccacg gggctcgccg ccgtcgcccc cacttacgag
     2701 ttgttcgccc tcgcgctgat cccctgcggg ctcgcggcgc tgacggcgtt gacgacggcg
     2761 aacacgctcg tccagttgtc ggtcgacccg gcgatgcgtg gccgggtgat ggccctctat
     2821 atggcgatct tcctgggcgg cacgccgatc ggggctccgc tcatcgggcg gctcggcgac
     2881 tgggcggggc cgcggtggac gatcggcgtc gggtcggtcg ccgtcgggtt gagtgtggtc
     2941 atcgtcggtg gctggctggt caagagggag aatgttgaca tgttgatccg caagcggggc
     3001 cgtcgatgaa cgtccggttc cggcagcggc tgaccttcgc cgtgttggtc ctgctcgtcg
     3061 tcctcggggt ggccgccgcc ctcgtgaggt gaccgggcgg cataccccca ggtgcgcggg
     3121 cggcataccc ccgcgttggt ccgccccgcc cccgccccca accccacaac ggagatagcg
     3181 ctcgcacggt catctttccg gcgtttgaag cccttcagac acggaaaagt gaccgtgcga
     3241 gcgctatctc cgggtcaaga ggggtcagcg ggggccaaga ggggtcaggc gggtcagacg
     3301 gggagccggc gatcagagct ggccgacgac ccagtcgatg ctggcggtga gggccgagat
     3361 gtcctcgggg tcgatggccg ggaacatcgc gatccgcagc tggttgcggc ccagcttgcg
     3421 gtagggctcc acgtcgacga tcccgttggc gcgcaacacc tttgcgacgg ccgccgcgtc
     3481 caccgtgtcg tcgaagtcga tcgtcccgac cacctgcgag cgggctcccg ggtcggagac
     3541 gaacggcgtc gtgtatgccg tgcgctcggc ccagccgtag aggcgcgagg cggagtcggc
     3601 ggtgcgaccg gtggtgaagg ccagcccgcc gttgccgttc atccagtcca actgcgccgc
     3661 gaacatcgcc agcgtggcga ctgcgggggt gttgtaggtc tggttcttca gcgagttgtc
     3721 gatcgcggtg ggcagcgaat agaagtcggg cacccagcgg ccgctcgccg tgatctcctc
     3781 gacccgggcc agggcggcgg gggagaaggc ggccaaccaa aggccaccgt cggaggcgaa
     3841 gcacttctgc ggagcgaagt agtagacgtc ggcctggctc atgtcgaccg gcaggccacc
     3901 ggcgccggag gtggcgtcga tgaggacgag cgcgtcggca tcggcgccct cgacccgctg
     3961 gaccggggcc atgacgccgg tcgaggtctc gttgtgcggc caggcgtaga cgtcggcgcc
     4021 ggcctcggcc acggcggtgg ccagggtgcc gggggcggcc ttgatgatcg tcgactcgcc
     4081 gaggaacgga gccgtgttgg tcaccgaggc gaacttgttg gagaactcac caaagctcag
     4141 gtgctgggcc ttggcgcgca ccaggccgaa cgcggcgatg tcccagaagg ccgtcgaccc
     4201 accgttgccg aggatgatct cgtagccctc ggggaggctg aacaggtcgc gcagtccgga
     4261 gcggatctca ccgaccaggc ccttgaccgg cgcctggcgg tgggacgtgc cgaggatcgt
     4321 gccgccgatc tcactgaggc gggccacctg ctcggggcgg accttgctcg ggccgcagcc
     4381 gaagcggccg tcgccgggga gcagatcggt ggggatggtg agggtggcgg gctggtcggt
     4441 cacgatcgtt caaccttcga tggcgggtcg tcgtgccggg cgccgggccg gcgcggggca
     4501 cgtcaagtgt cccaacccgg cgcggcggcg ttgccagcgg gtccaggtcg tggacgcgtg
     4561 gccccgcgcg cgcaggcggg agccgggctc gatgtggtgc gtgtcacttc ctgggtgggg
     4621 cgcctgggaa caactgccga agtgcacacg ttctgcacct gtaacgttgt aatcagtgca
     4681 agagaggaat caccatgcac ccgcatcatc actcttcgtc agcagacggc gaccacccca
     4741 ccgacgccga gcccaccgag ggccgccggc gcggcccggg acggcgccgc ggcccagact
     4801 tcgggatggg tttcggtccc gcgttcgcca tgggtgccgc aggccccgac ggtcctggta
     4861 tgccgttcgg ccccggaggc cccggaggac ccggcggccc cggcggcccc ggaggacccg
     4921 gcggtcgcgg tcgtggcccc ggcggtcgcg gccgtggtcg cggcggtccc ggtggacctg
     4981 gcccgttcgg tcgccccggc ggctggcagc aggccgatct cccgtcggcc gatgacgtcg
     5041 ccgaatggtt cgccggccgg ctcccggccg actggttcat cggggacgcg cagatcagcg
     5101 tcgaccgcga ggagatcgtc gtcgtcggtg agctgccccc agtcgacggt cagggcgaca
     5161 gcgctgctgc tgccgagggc cggatctcga ggttccggga gcagtcccgg gacgagcgga
     5221 tcgccatcgc cacccaggcg caggagcgct acggccgcaa ggtcagctgg ggcgctcgca
     5281 tcggggagcg tcgtgagctg ttcacccacc tcgccgtgcc ggtcatgacc cggctgcgtc
     5341 agcccgagcg tcaggtgctc gacaccctcg tcgacgccgg agtcgcccgg tcgcgctcgg
     5401 aggcgctggc ctgggcggtt gcgctggtcg gccagcacac cgagacctgg ctgtcggacc
     5461 tgcgggccaa gatggccgag gtcgacaaat tgcgcgccca aggcccgcag ctgtaatcgc
     5521 tgcacgcgca cggcatacca agaacagcaa agccgcggcc ccgggacggt gagtgtcccg
     5581 gggccgcggc agctgtaggc gtcagtgcac ctcgtgcggg tcgatgtggc gcggccatcc
     5641 ggggacgtcc tcgggccggc gctcatggat cccgatgtag cgggcggccg ggcggatgag
     5701 ccgcgcggcg cgcttctgct cgaggatgtg cgccgaccag cccgcggtgc gcgcgcaggt
     5761 gaacatcggc gtgaacatcg acggcggcac ctcggcgtag tcgaggatga ccgcggccca
     5821 gaactcgacg ttggtctcaa gcacccggtc gggtcggcgc tcgcgcagct cggcgagcgc
     5881 ggcgacctcc agggcgtggg cggcctcgta gcgcggcgcg tcgagccgct tgcacgtggc
     5941 gcgcaggacg cgggcccgcg ggtcctcggc gcggtagaca cggtggccaa agcccatgag
     6001 gcgctcgccg cggtccagaa cgcccttgac gtatgccgtt gcgtcaccga ggttttcgac
     6061 ggcttcgagc atgtgcagca cccgggacgg ggcgccgccg tgcagggggc cgctcatggc
     6121 gccgaccgct ccggagaggc aggcggcgac gtcggcgccg gtggaggcga tgacccgcgc
     6181 ggtgaaggtc gaggcgttca tgccgtgctc ggcggccgac acccagtagg cgtcgacggc
     6241 ctcgacgtgc cgcgggtcgg gctcgccgcg ccagcggatc atgaaccgct cgacgatggt
     6301 gcgggccttg tcgacctcac gctgggggac catcggcttg tggacgccgc gggccgactg
     6361 gccgacgaac gacagcgcca tgacggcggc ccgggcgagg ttctcgcggg cctcttcttc
     6421 gtcgatgtcg aacagcggcc ggaaacccca gatcggggca agctgggcca acgccgactg
     6481 cacgtcgacg cggacgtcgc cggagtggac cggcagcggg aacggctcgg cgggcggcag
     6541 gcccggctcg aacgcgccgt cgacaagcag cccccatacc tggccgaacg acaccttgtc
     6601 gacgaggtct tcgatgtcga ccccgcggta gcgcagggcg ctgccttcct tgtcgggttc
     6661 ggcgatctcg gtctcgcagg cgacgatccc ctccagaccc ggcttgaacc ggtcttcggg
     6721 ggcggcgagc ggttgggtgg tcacagggca actcctcgga acagtggggc agtgaagccc
     6781 tagcgattct gccccatccc cccgggcacc tgggaccgcc tgcccaggga acgggccggg
     6841 gcaggcgcct aacgcgtgcg cttgagcgcc gcgcgggcct tgccggccgc ctgtcctccc
     6901 gcggaggtga tgcggtcctt ggctgtcacg gcaggctcgg ggacgaaacg ctggctgagg
     6961 tcggctcgct cgacgagttg ggcgagggtc gcctcgacga cggggtggga cgcgattggc
     7021 tcctcgccgc tgtagacgac ccacaccggc tgaccctggt ggaagacccg cagcacggtg
     7081 ccggcctgca gggtggtcgc ctccgcagcc gccgcacgcc gcgacttctg ctcggcgagc
     7141 ttcttctggg cgtatgccgt cgccggctcc ttgagcgcct tggccgcgac gatgacgtgc
     7201 ggaccgtact tgacgccgta tttgaccgcg ttcccgacga ttgtgctctt ggccatgtgt
     7261 gcctggaccc ttccccgcgc cgtctattga cgggaagcct acgctcggag ggtgaccgac
     7321 cgcgtcgact acaccggcga ggggctcgac gaagccgacc tcgcgagcac gccctacgca
     7381 caactcaccc gctggatcgg ccaggcccag gcggaaggag tccgagccgc cgaatccccc
     7441 gaggggctgg cgctgcagtt cgccaccgtc gatgctgctg gcgcgcccaa tgtgcggacg
     7501 gtgttgatgc gtttcctcga cccgcgcggg ccgggttttg tcaccgatgc cgggtcgatc
     7561 aagggccgcg aaatcgctgc gaaccccgca gtggccgcgt cgctcggctg gccggagttg
     7621 ttccgggtcg tgcgcttccg cgggctggcc gagccgatcg agccggaggc gctcgccgac
     7681 tacttcagcc agcggccgtg gggctcgcgg gtcgccgcgt gggcgtcgca ccagtcggag
     7741 caggtcgcca gccgggccgc cttgctcgcc gaattcgaca ggtatgccgc gcagttccct
     7801 gaccggggca acccggacga cgtgccggtg ccgccgtcgt ggtcgggctt ccggatccgg
     7861 gcgaccgagg tcgagttctg ggctgggcgt tccagccgcc tgcacgaccg gatcgtgttc
     7921 acggcaccgc cggccgctga gttgggtgcc ggcgcgggcc aggttccgct cctggacgac
     7981 ccgagcgcgt ggagggtgtc acgccgccag ccgtgacggc ataccccggg gatgggaacg
     8041 gcccgtgggg aagccggtct ccgctgggcg gaggtccgac gtgcacgctg gacgaggcgt
     8101 gcctacccac gagccgcggc gactgctgga attgcggtcg cgtcgacgtc ccatagctgg
     8161 gacggtgcgc taacgcgcag ccacctcacg tgtccgatag gaactcacta cctgaccacc
     8221 tcccttcttc gtgtaccgca gacggtaagc cgcagtgcgg gcgattttca aggggtttct
     8281 gcgagctcgg ggagggtcgc gagcgtcgcc agggtttcgg cggcccaggc gaggtgggcg
     8341 tgcgcggcat actccccggc tgagagcgtg agcagccggt atgccgccga cgcggctgcc
     8401 gtgtcgtcgt tggccgcgcg cgcggcggcg atctcggcct cggtctcggt gcggatcgtg
     8461 gcgtaccccg cgagcatcgc ggaggcccgg tcggcggact cctgaacgag cacccggcag
     8521 tgggccgacc cgagctgccc tccgaggaag aggcgcagca gcaagccgtt gcgcggcggg
     8581 gagaccgcgg ccggctcggc gagcaactcc cgtaaccggg tgcggccggc ggcggtgagc
     8641 tcgaaacggc tccccgacgt gcgaccgggc tcggtggggc ggatctcgcc gcgggcgatg
     8701 agctcggtga gcgtggggta gatctggccg aagctctcgc tccagaagtg gcccagggtc
     8761 tgggtgatgg cggcgcgcac ggcatacccg gtcatcggct cgaccgacag ggcgccgagc
     8821 acggcgagat gggtttgcga ggacctggcc atgcgtttga ttatatctga cagatatgtc
     8881 aaggtgggtg ctgggcaaga tctctcacag ataggttcat gacgatggcg agcgatgcgg
     8941 tgacgccagt aacgccgacc tggccacgaa aggccgccgc ggccacctac gcgggggtcg
     9001 ccgggttcca agttgcgctc gccgcggggg cgccttgggg tgtcgcggcc tggggcggcg
     9061 ctcacgaggg cgtcctcccg atgggtttcc gcgtcggcag tgtggtctcg gccggggtgt
     9121 gggggttggt cgccgcgtcg ctgggcacgt cgtggccgcc ggcccgttgg cggcgcccgg
     9181 tcctcaccgg cttggcggtg ctcgcgccgg tgtccttcgc gatgaacctc gccacgccgt
     9241 ccccggtcga acgggccatc tgggccccga tcacggccgc gcagaccgtc ctcgtcctgc
     9301 tcgccttccg cgccgagcgc cgcgctcgct gacgcctccg ggagcgcgga accctccgcc
     9361 gcgcctcggc cgaacggcat acagtgcagc gagtgagcag caccccccag gtgaccagtc
     9421 tgcggccgat cgcggtcgcg gcatacgggc cgacggtgct gtcgtcgatc gggaccggcg
     9481 ctgtcctgcc tgtcctggcg ctcaccgctc gcgatctcgg cgcgagtgtc gggacggctg
     9541 cggtcatcgt gtcgctgctc ggggtgggga tgctcgtcgg cga
//
LOCUS       DAOMZY010000110         9504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_6824_length_9504_cov_6.571912, whole genome shotgun sequence.
ACCESSION   DAOMZY010000110 DAOMZY010000000
VERSION     DAOMZY010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9504
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6824_length_9504_cov_6.571912"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..855)
                     /locus_tag="PLK64_07745"
     CDS             complement(94..855)
                     /locus_tag="PLK64_07745"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011209034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HPV79676.1"
                     /translation="MPRNPLLPDLSGKVAVVTGGSRGLGRAMAFGLAHAGAQVVVASR
                     HGDQCEATAAELTEATGIPALGLGAHVGKWSELDGFVEEVYARMGRVDVLINNAGMSP
                     LYDTVTDVTEELFDKVIAVNLKGPFRLSALVGTRMAAGEGGSIVNISSAAAIHPRPHT
                     LPYAAAKAGLNALTVGLAHTFGPSVRVNAIMAGTFLTDVSRSWDPEVFARRAEGFALR
                     RGGQPEEIVGTALYLASDLSSYTTGAVITVDGGQP"
     gene            956..2143
                     /locus_tag="PLK64_07750"
     CDS             956..2143
                     /locus_tag="PLK64_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPV79677.1"
                     /translation="MSGFDLTAEQEQLKAVAAKVAADVYAAPAARWDLERTSLPREEV
                     ARLADLGYLGLAIPEEYGGQGGTLVDALVVIEELAKVCRPAAFQVFECNTGPIRVIQF
                     FGTEEQKQRWLTASCRGEQTMAIAISEPDAGSAATDLRTKARRDGDHWVINGAKRWIS
                     NGGHADSYLVYCRMSDQPGAKGIGAIVVPADTPGFSTGAQEKLMGFRGIPSADLFFDD
                     VRVPLDHLVIEEGGFGKLFGVFSIERLGNATMSLALGQAALDKSTAYVQERVQFGRPL
                     IDFQNVQMTIAQMAVQVEAARLLVLRASSEAGDGLPDTYQVSVAKCFANEMAKQVTDL
                     AMQLHGGNGYTEEYGLERLHRDAHGWAIAGGTPAIQRVRIASELLGRTFNQRPERAAN
                     GTS"
     gene            2140..3339
                     /locus_tag="PLK64_07755"
     CDS             2140..3339
                     /locus_tag="PLK64_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPV79678.1"
                     /translation="MTTEGALFELPARYRQLQDEARALALSCADIAGRADEADRIDPE
                     MRARLAASGLAEVTVAAAYGGRFEAVDSLAVTVVREVLAGVSAHLDSLFAMQGIGSFP
                     VSTAAAEPLRQEWLPRVARLEAIGALGLTEPDVGSDLRAITTTVRQEGDELVVDGHKS
                     FITNAGDATFYSILAREEAGYSLVFVPADTPGLSVTHPHQIIAPHVLGDVILEGVRVP
                     VGNRLGAPGAGFKLMLATLATFRVSVAGAAVGVAQAALDEAVAHASGREQFGMPLARL
                     GPVPAMLAQSWVDVESARCTAYRAAAAAARDPLSALHLSSIAKVAATEAAARVTDRSV
                     QVMGRFGLVRGSVIERLYREARPMRIYEGATEVILDSLARKLVKDAATATSAAATGSG
                     ASAGDAG"
     gene            3336..4121
                     /locus_tag="PLK64_07760"
     CDS             3336..4121
                     /locus_tag="PLK64_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPV79679.1"
                     /translation="MIIDAHAHVWPDHIAAVVLATRPSGLDSHFDGTLTGLLTTMDAA
                     GIDLSMALAVANKASTVMRTNEWIGQIPRDRLIPAGTVHPGLSPEENVASLRDNGIRA
                     VKLHPLFQDVAFADPQVIDIMHALAADGVTVLTHVGSGGDAAANERGNPVALRALIDA
                     VPTLRLVAFHYGGYHELDTAQELIVGAPVLLETSWPPSVATLDPQVVRDLIRRHGVDR
                     VVFGTDWPMTDPAAEIAAIRALGLEPDEEAAILGGNLARLLGL"
     gene            4130..5278
                     /locus_tag="PLK64_07765"
     CDS             4130..5278
                     /locus_tag="PLK64_07765"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HPV79680.1"
                     /translation="MTDAWIVGAVRSPVGRRGGALSTVHPADLGAHVLRALVERTGVD
                     PGEIDDVLWGCVGQVGAQASNIGRTTVLSAGWPEHVPAMTIDRQCGSSQQALHAAAHQ
                     IRAGDADLVVAGGVEVMSQVPIASPTRLGLEAGMAHPRGGADWAARYGDEEISQFRGA
                     ELIAKRWGLDRARMERFAVESHRRAIASTAAGDFEAEIAPLPGLAVDEGPRSDTSAER
                     LAGLTPLQPGGRITAALASQVSDGAAALLVASDRAVDRNRLTPLARVHTMAVVGSDPV
                     LMLTGPIPATATVLHRTGLSLAEIDTIEINEAFASVVLAWADETDADLDRVNPQGGAL
                     ALGHPLGATGARLMTTLVHRMGRDHLRYGLQLMCEGGGMANATVLERA"
     gene            5280..6815
                     /locus_tag="PLK64_07770"
     CDS             5280..6815
                     /locus_tag="PLK64_07770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV79681.1"
                     /translation="MTSPAEEAHGIPSAGSPPAPPAEERGIPSAELPSAPPPRGLVPC
                     LVYAALSGSVVAGLGNPIVVPVNRTLHVSLEAAQWTLTMSLLMGVVATPIVARLADGR
                     LRRRVLVGALATVAIGSLVGALVPNFPGLMVARALHGLGYALVALTVGIAREHLHGPQ
                     LHRTLAVLSTSLAVGAGVANPTVGLMVAIWDYRAGFVFAFLVAAAGAWWTFRTVPEPK
                     AGIAPVHTDVLGAVLLCSGLGFGLLAITHGVSWGWTSSGVLGLAGGAVVLLLAWVLIE
                     VRLAHPLVDVRLAVAPGILGANLAALLIGCSVFAGAAAGIILTQQTPSGGVGLGHNVA
                     ITGLLMGPMALGSLAGPPIARRLVRRLGERSVLPIGTLSCALGFGIFARWHHTELEVL
                     LMMGLMGLGVGITYSILPSLVVARVPMQRTASATGVNQVLRLLGGAVGSAGLAAVLAA
                     QVDGASGLPAESGYVMASLLAAGVSLVAAPLSYWLVPDAPAGASEAGGERTAYLPEEP
                     VTP"
     gene            6820..6960
                     /locus_tag="PLK64_07775"
     CDS             6820..6960
                     /locus_tag="PLK64_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79682.1"
                     /translation="MLAAGVRCTAPEDVAEQLIGLQEELGMTHFVDKPVFGTQVIPAV
                     TR"
     gene            6957..7793
                     /locus_tag="PLK64_07780"
     CDS             6957..7793
                     /locus_tag="PLK64_07780"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPV79683.1"
                     /translation="MKLETGQVAVVTGAASGIGRGLVEAFVARGLSVVGVDVEAGALD
                     AMVAEVNGGAGDGTGESGTGQVVGRVVDVRDPEALLALAADVVATFGRVDVVCNNAGV
                     ITSRLSTWEQPHSDVQWTTEVNFYGVVNGVRAFVPHFIAAGHGHVVNTASVAGLSTIP
                     GGGQAAYSASKHAVVGLSETLALELATVAPEVGVTVLCPGQVPTKIRDARRNRPADRP
                     ETGSSTVLPDFRLTMDVATPAEVAEMVLAAIEDGLFYLVTAPDAGQWARTRAQGVIDG
                     LS"
     gene            complement(8059..8445)
                     /locus_tag="PLK64_07785"
     CDS             complement(8059..8445)
                     /locus_tag="PLK64_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79684.1"
                     /translation="MRKEQKRAATILGTVVGLGPNVAIFRVSRTTPALDARRATVHAL
                     AELACQLRADRVVFDTIDSMSARDRAWVIEGVHLAGEPTPPFGYHHQRRHEEPLLWIP
                     DAVGWAWARGGQHRRAVERICYLVDL"
     gene            complement(8685..9440)
                     /locus_tag="PLK64_07790"
     CDS             complement(8685..9440)
                     /locus_tag="PLK64_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79685.1"
                     /translation="MPGPSETEGIGMAMLRGVLRVSPIGDYRTLPALLGVDFLVVDEP
                     GGHGSGPFHPIVEVEDLAALYEMLRTERIVVGVLTPLDDGQRHALTVRDLRGMALLTV
                     TGDLSPTHRLLTGPDVRAHVRLPFDPRTNVLDPCTWVLAEVGRKLTGESVVGIDMQPV
                     DRDRRAVFVQDQRGRRVLVGEVRVMRGSEYLDMDVTLDGGLVTNVVVEVVHDASDAMV
                     SIVHSGWVVGSEEQRGQFDGWQDALSAWAASQT"
BASE COUNT         1398 a         3340 c         3284 g         1482 t
ORIGIN      
        1 ggcatacaag cgtcaccaac ctcatacaag cgagcgcggc gcccgcccgg gcgcgcgggc
       61 ccgccagacc cgtcggccaa agtccaaacc gacctacggt tggccgccgt cgacggtgat
      121 gacggccccg gtcgtgtagc tcgacaggtc actcgccaga tacaaggccg tccccacgat
      181 ctcctccggc tgcccgccgc gccgcagcgc gaacccctcg gcccgccgcg cgaacacttc
      241 ggggtcccac gagcggctca cgtcggtgag gaacgtcccg gccatgatcg cgttgacccg
      301 aacgctcggc ccgaaggtat gcgcgaggcc cacggtgagg gcgttgaggc cggccttggc
      361 ggcggcatac gggagggtgt gtgggcgcgg gtggatggcc gcggcgctgg agatgttcac
      421 gatcgagccg ccctcccccg ccgccatccg ggtgccgacg agcgccgaca gccggaacgg
      481 ccccttgagg ttgaccgcga tgaccttgtc gaacaactcc tcggtcacgt cggtcaccgt
      541 gtcatagagc ggcgacatcc ccgcgttgtt gatgagcacg tccacgcgcc ccatccgcgc
      601 gtagacctcc tcgacgaacc cgtcgagctc ggaccacttc ccgacgtggg caccaagccc
      661 gagagcgggt atgccggtcg cctcggtgag ctcggccgcg gtggcttcgc actggtcacc
      721 atgacggctc gcgacgacga cctgggcgcc ggcgtgggcg agcccgaagg ccatggcgcg
      781 acccaggccg cggctgccgc cggtgacgac ggcgaccttg ccggacaggt cggggaggag
      841 ggggttgcgc ggcatacggg ctcctgcagg tcggttgtct cagactactt gactgacggc
      901 aattcatcgc tatggtgatc atagttatca tcaagccgcg atcaaggagg ccagcgtgag
      961 tgggttcgat ctcaccgcgg agcaggagca gctcaaggcg gtagcggcca aggtcgcagc
     1021 ggacgtctat gccgccccgg cagcccgctg ggacctcgag cgcacgtcgc ttccccgtga
     1081 ggaggttgct cgcctcgccg acctcggtta cctcggtttg gccatccccg aggagtacgg
     1141 cggccagggc ggcaccctcg tcgacgctct cgtcgtcatc gaagaactgg ccaaggtctg
     1201 ccgcccggcc gccttccagg tcttcgagtg caacaccggg ccgatccggg tcatccagtt
     1261 cttcgggacc gaggagcaga agcagcgctg gctgaccgcc tcgtgccgcg gcgagcagac
     1321 gatggccatc gccatctccg agcccgacgc cggatcggcc gcaaccgacc tgcgcacgaa
     1381 ggcccgccgc gacggcgacc actgggtcat caacggcgcc aagcgctgga tctccaacgg
     1441 tggccacgcc gacagctacc tcgtctactg ccggatgtcc gaccagccgg gcgccaaggg
     1501 catcggcgcg atcgtcgtcc ccgccgacac tcccggcttc tcgaccggtg cccaggagaa
     1561 gctcatgggc ttccgcggca tcccgagtgc cgacctgttc ttcgacgacg tccgcgttcc
     1621 cctggaccac ctggtcatcg aggagggcgg cttcggcaag ctcttcgggg tcttctcgat
     1681 cgagcggctc ggcaacgcga cgatgagcct agccctcggt caggcggcgc tggacaagtc
     1741 caccgcctat gtccaggaac gcgtgcagtt cggtcgcccg ctcatagact tccagaacgt
     1801 ccagatgacc atcgcgcaga tggccgtgca ggtcgaagcg gcacgactgc tcgtcctgcg
     1861 ggcctccagc gaggcgggcg acgggctgcc cgacacctac caggtgtcgg tcgccaagtg
     1921 cttcgccaac gagatggcca agcaggtcac cgacctggcg atgcagttgc acggcggcaa
     1981 cggctacacc gaggagtacg gcttggaacg gctgcaccgc gacgcccacg gctgggccat
     2041 cgctggaggc actccggcga tccagcgggt ccggatcgcc tccgagttgc ttggacgcac
     2101 gttcaaccag cggccggagc gcgccgccaa cggcacctcg tgaccaccga aggcgccctc
     2161 ttcgaactgc cagcccgcta ccggcagttg caggatgagg cccgcgccct cgccctgtcc
     2221 tgcgccgaca tcgcgggccg cgccgacgag gccgaccgga tcgaccccga gatgcgcgct
     2281 cgactggccg cctccgggct ggccgaggtc actgtcgcgg cggcatacgg cgggcggttc
     2341 gaagccgtcg actcacttgc ggtgaccgtg gtccgcgagg tgctggcggg cgtgagcgcc
     2401 cacctcgact cgctcttcgc gatgcagggc atcggcagtt tccccgtgtc gaccgcggcc
     2461 gccgagccgc tgcgccagga gtggttgccg cgggtcgccc ggctcgaagc gatcggcgcg
     2521 ctcggtctca ccgagcccga cgtcggctcc gacctgcgcg cgatcacgac gacggtccgc
     2581 caggagggcg acgagttggt cgtcgacggg cacaagtcgt tcatcaccaa cgccggcgac
     2641 gcgaccttct acagcatcct cgcgcgcgaa gaggccggct attcgctggt tttcgtccca
     2701 gcggacaccc ctggcctcag cgtcacgcac ccccaccaga tcatcgcccc gcacgtcctc
     2761 ggcgacgtca tcctcgaggg cgttcgggtg ccggtcggca accgcctcgg cgcgcccggg
     2821 gcgggtttca agctcatgct cgcgacgctg gcgaccttcc gggtctcggt cgcgggcgcc
     2881 gctgtcgggg tggcccaggc cgccctcgac gaggccgtcg cgcacgcatc cggacgcgag
     2941 caattcggta tgccgctcgc gcgcctcggc cccgtgcccg cgatgctcgc gcagagctgg
     3001 gtcgacgtcg agtcggcgcg gtgcacggca taccgggcgg ctgcggctgc cgcgcgcgat
     3061 ccgttgtcgg ccctgcatct ctcctcgatc gccaaggtgg ccgcgacgga agctgcggcc
     3121 cgcgtcaccg accgctcggt gcaggtgatg gggcggttcg ggttggtgcg cggcagcgtc
     3181 atcgagcggc tctaccgcga ggcgcggccg atgcggatct atgaaggcgc gaccgaggtc
     3241 atcctcgaca gcctggcccg caaactcgtc aaggatgcgg cgacggcgac ttcggccgct
     3301 gcgaccggca gcggcgcgtc ggcaggggac gccggatgat catcgacgcc cacgctcacg
     3361 tgtggcccga ccacatcgcg gccgtcgtcc tggccacccg gccgtccggg ctggacagcc
     3421 acttcgacgg cacgctcacc gggctgctga cgacgatgga cgcggctggg atcgacctgt
     3481 cgatggccct ggccgtcgcc aacaaggcct cgacggtgat gcgcaccaat gagtggatcg
     3541 gccagatccc ccgcgaccgg ctcatccctg cgggcaccgt gcatcccgga ctgtcacccg
     3601 aggaaaacgt cgcctcgttg cgggacaacg ggattcgcgc ggtcaagctg cacccgctct
     3661 tccaggacgt ggcgttcgcc gacccccagg tcatcgacat catgcacgcg ctggccgccg
     3721 acggggtcac cgtgctcacc catgtgggct ccggtggcga cgctgcggcc aacgagcgcg
     3781 gcaaccccgt tgccctgcgc gcgctcatcg acgcggtgcc gacgctgcgc ctcgtcgcat
     3841 tccactacgg cggctaccac gagctcgaca cagcccagga gctcatcgtc ggtgcgccgg
     3901 tgctgcttga gacgtcgtgg ccgccgtcgg tcgccaccct cgacccgcag gtcgtccgcg
     3961 acctcatccg tcggcatggt gtcgaccggg tcgtcttcgg caccgactgg ccgatgaccg
     4021 atcccgccgc cgagatcgcg gcgatccggg cgctcggact cgagcccgac gaagaggccg
     4081 ccatcctcgg cggcaacctt gcccgcctgc taggtctcta ggccggcgaa tgactgacgc
     4141 gtggatcgtc ggcgccgtcc gctcccccgt cggtcgacgc gggggcgccc tctcgacagt
     4201 tcaccccgcc gacctcggcg cccacgtgct gcgtgcgctc gtcgagcgga ccggggtcga
     4261 cccgggcgag atcgacgacg ttctctgggg gtgcgtcggc caagtcgggg cgcaggcctc
     4321 caacatcggg cgcaccaccg tgctgtcggc cggatggccg gagcacgtgc ctgcaatgac
     4381 gatcgaccgg cagtgcggct cgtcgcagca ggcactgcac gcggccgccc accagatccg
     4441 cgccggtgac gccgacctcg tcgtcgccgg cggtgtcgag gtgatgagcc aggttcccat
     4501 cgcctcacct acccgactcg ggctcgaggc aggcatggcc cacccgcgcg gtggagccga
     4561 ctgggcggcc cgctacggcg acgaggagat ctcgcagttc cggggcgccg aactcatcgc
     4621 caaacgctgg gggctggacc gcgcgcggat ggagcggttc gccgtcgaga gccaccgcag
     4681 ggccatcgcc tcaacggctg caggcgattt cgaggcggag atcgcaccgc tgcctgggct
     4741 cgcggtcgac gaaggccccc gctccgacac ctcggccgag aggctcgctg ggctcactcc
     4801 cctgcagccg ggcggccgga tcacggcggc cctggccagc caggtgtccg acggcgccgc
     4861 cgcactgctc gtcgcctccg atcgcgcggt cgaccggaat cggctgaccc cgctcgcgcg
     4921 cgtacacacc atggccgtcg tcggctccga cccagtcctc atgcttactg gcccgatccc
     4981 cgccacggcg acagtcctgc atcgaaccgg gctgtcgctg gccgagatcg acacgatcga
     5041 gatcaacgaa gccttcgcgt ccgtcgtcct cgcctgggcc gacgagaccg atgctgacct
     5101 ggaccgcgtc aatccccagg gcggggccct cgctctcggc cacccgttgg gtgccaccgg
     5161 cgcccggctg atgacgaccc tcgtccaccg aatgggccgc gatcacctgc gctacggcct
     5221 gcagctcatg tgcgagggtg gcggaatggc caacgccacc gtcctggaac gcgcctgagg
     5281 tgacctcacc ggccgaggag gcgcacggca taccctcggc agggtcgcca cccgcaccgc
     5341 cggcggaaga gcgcggcata ccctcggctg agctgccgtc cgctcctcca cctcgcgggc
     5401 tggtcccctg cctggtgtat gccgctctca gcggctcggt cgtcgcgggc ttgggcaacc
     5461 cgatcgtcgt cccggtcaac cggaccctgc acgtctcgct cgaggcggcc caatggacgc
     5521 tgacgatgag cctgctcatg ggggtcgttg ccaccccgat cgtcgccagg ctggccgacg
     5581 ggcggctgcg gcggcgagtg ctcgtcggcg cgctggcaac ggtggccatc gggtcgctcg
     5641 tcggtgcgct cgtgccgaat ttccccgggc tgatggtggc gcgggcgctg catgggctcg
     5701 gctatgcgct ggtcgcgctc accgtcggga tcgcccgcga gcacctgcat gggccgcaac
     5761 tgcatcggac cttggccgtg ctctcgacga gcctggccgt cggtgccggc gtggccaacc
     5821 cgacggtggg cctcatggtg gcgatctggg actaccgggc cgggttcgtc ttcgcgttcc
     5881 tcgtcgcggc agcgggggca tggtggacgt tccgcactgt ccccgagccg aaggcaggca
     5941 tcgcgccggt gcacaccgac gtcctcgggg ccgtgctgct gtgcagcggg ctgggtttcg
     6001 ggctgctggc gatcacccat ggggtgtcct ggggctggac gtccagcgga gtgctcgggt
     6061 tggccggtgg ggccgtcgtg ctgttgctgg cctgggtgct catcgaggtg cggttggctc
     6121 acccgttggt cgatgtccgg ctcgccgtcg caccggggat cctcggggcg aacctcgccg
     6181 cgctgctcat cggctgctca gtgttcgccg gggctgcagc ggggatcatc ctcacccagc
     6241 aaacgccctc cggcggagtc ggtttgggcc acaacgtcgc gatcaccggc ctgctcatgg
     6301 ggccgatggc gctgggcagc ctggccggtc cgccgatcgc ccgacggctg gtccggcgcc
     6361 tcggcgagcg gtcggtgctg ccgatcggaa cgctgtcctg cgcactgggt ttcgggatct
     6421 tcgcccgctg gcatcacacc gagttggagg tcctgctgat gatgggcctg atgggcctcg
     6481 gcgtcggcat cacctattcg atcctgccca gcctggttgt cgcccgggtg ccgatgcagc
     6541 ggaccgccag cgcgaccggg gtcaaccaag tgctccggct gctcggcgga gcagtggggt
     6601 cggcggggtt ggccgccgtg ctggcggcgc aggtcgacgg tgcgagcgga ttgccggcgg
     6661 agagcggcta tgtcatggcc agcctgctcg ccgcgggggt gtctctggtg gccgcaccgc
     6721 tgtcctactg gctggtcccc gacgccccgg caggtgcgtc ggaggcgggc ggggagcgga
     6781 cggcatacct cccggaggag ccggtcacgc cctgaacggg tgttggctgc gggagtccgg
     6841 tgcaccgcac ccgaggacgt cgctgagcag ttgatcggac tacaggagga gttgggaatg
     6901 acgcacttcg tcgacaaacc ggtgttcggc acccaggtca tcccggcggt gacccggtga
     6961 agctcgagac gggccaggtc gcggtcgtga ccggcgccgc gagcgggatc gggcgcggtt
     7021 tggtcgaggc cttcgtggcg cggggactgt cggtcgtcgg ggtcgacgtg gaagccggcg
     7081 cacttgacgc gatggtggcc gaggtcaacg gcggcgcggg tgacgggacg ggcgaatcag
     7141 ggactgggca ggtggtcggg cgggtcgtcg acgtccgcga ccccgaggcg ctgttggcgc
     7201 tggctgccga tgtcgtggcg accttcggcc gggtcgacgt tgtgtgcaat aacgccggcg
     7261 tcatcacgtc gcgtctgtcg acatgggagc agccgcacag cgacgtgcag tggacgaccg
     7321 aggtcaactt ctacggcgtc gtcaacgggg tgcgagcctt cgtgccgcac ttcatcgccg
     7381 ccggtcacgg acatgtcgtc aacaccgcct cggtcgcggg actgtcgacg atccccggag
     7441 gcgggcaggc ggcatacagt gcctcgaagc acgccgtcgt cgggctgtcg gagacgctcg
     7501 ctctggagtt ggccaccgtc gcgcccgaag tgggtgtcac cgtgctgtgc cccggccagg
     7561 tgcccaccaa gattcgcgac gcgcggcgca accggcccgc cgaccggccg gagacgggga
     7621 gcagcacagt gctgccggac ttccggctca ccatggacgt ggcgactccc gccgaggtag
     7681 ccgagatggt gctggcggcc atcgaggacg ggctcttcta cctcgtcact gctcctgacg
     7741 ccggccagtg ggcccgcacc cgcgcccaag gagtcatcga cggcctgtcc tgagttgacc
     7801 gtcggcttgc gacgccccat gtctgccctc gccgcatccc atcgtttccc ttcgcgcgtc
     7861 gcggcatcca cctgggggca ctccaacggg gagtacctca ggtacgcagt gcgtacctga
     7921 ggtacgtgat ccctgcaggg gtcctgatca caacagctag ggcaaagcgg ctatgcttga
     7981 agttacgaag gggaattacc ccccaagtag tgagcccgat acccgtccgg acggtcgcgg
     8041 tgtcgggctt cgcgctgtct acaggtcgac caggtagcag attcgctcga ccgcgcgacg
     8101 atgctgaccc cctctggccc acgcccagcc gaccgcatcg ggtatccaga gcaggggctc
     8161 ctcgtgacgg cgctggtggt gatagccgaa cgggggagtt ggctcgccgg cgaggtgaac
     8221 tccttcgatg acccacgctc ggtcacgggc ggacatggag tcgatggtgt cgaagacgac
     8281 acggtcagcg cgaagttggc atgccaactc agcaagggcg tggacggtgg cacgtcgggc
     8341 gtcgagggcc ggagtggttc tggagactcg gaagatggcc acgttggggc cgaggccgac
     8401 gacggtgccc agaatggtgg cggcgcgttt ctgttccttt cgcatgtgga ggctgcggct
     8461 gccgggcagg agcaaccctc gcatgactga gcgcatctgt ggcgcgtcgg cgctcgcgac
     8521 cacggcgccc atcagcaggt agtccccggc cgtggactcg tcgacagtag atgtgcgctc
     8581 cgggcgtggg atcccacgag aggggagtca ccgttcttgc acgcctatgc gcggggcttc
     8641 cccccaaaag cggtgctggg tcccgcgaga gtcccgagaa cgcttcaggt ttgactggct
     8701 gcccaggcac tcagcgcgtc ctgccacccg tcgaactgac cccgctgctc ttcggagccc
     8761 acgacccagc cgctgtgaac gatgctgacc atggcgtcgg aggcgtcgtg gactacctcg
     8821 acgaccacgt tggtcaccag acccccgtcg agagtgacgt ccatgtccag atactcgcta
     8881 ccgcgcatca cgcgcacttc gccgacgagt acacgccggc cacgttggtc ctgcacgaac
     8941 acagcgcgcc tgtctcggtc gacgggctgc atgtcgatcc ccacaacgga ctccccggtc
     9001 agcttcctgc ccacctcggc gaggacccat gtgcaaggat ccagaacgtt cgtgcgtggg
     9061 tcaaaaggga gccgaacgtg ggcgcgaaca tccggtcccg tcagcaatct gtgcgtaggc
     9121 gagaggtctc ccgtcaccgt gaggagagcc atcccgcgca ggtcgcgaac cgtcaacgca
     9181 tggcgctgac cgtcgtcgag gggtgtcagc accccaacaa cgatccgttc cgttcgcagc
     9241 atctcgtaca gcgcagcgag atcctccacc tcaacaatcg ggtggaaagg gccggagcca
     9301 tgccctcctg gctcgtcgac cacgaggaag tcgacgccca acaacgcggg caaggtgcgg
     9361 taatccccga tcgggctgac ccgtagaacc cccctcagca tcgccatccc gattccctcc
     9421 gtctcactgg gccccggcat gacgtgcgcc cgaagctaac tagttggttg cggcgatacg
     9481 gcggcaggct gacaaccgcc gtcg
//
LOCUS       DAOMZY010000111         9496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_6834_length_9496_cov_5.404194, whole genome shotgun sequence.
ACCESSION   DAOMZY010000111 DAOMZY010000000
VERSION     DAOMZY010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9496
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6834_length_9496_cov_5.404194"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PLK64_07795"
     CDS             <1..397
                     /locus_tag="PLK64_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79686.1"
                     /translation="RTGATEVWAWPDEVELARFFTACRERELPFKLTGGLHHVLRADH
                     PGPDGGRQHGLLNVLAAVHATAEGVPEPEVAELLGERDAAPLVDLLTALSTDAASRVR
                     CAFTAYGCCTVADPLGELAALRLIDKEHQ"
     gene            394..1635
                     /gene="fahA"
                     /locus_tag="PLK64_07800"
     CDS             394..1635
                     /gene="fahA"
                     /locus_tag="PLK64_07800"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HPV79687.1"
                     /translation="MTWLDLPADHPFGLAHLPYGVFSTVADPAPRVGVRIGDLVLDAA
                     RAAAATGYAAGAAWATPSLNAFLGQGTTAWAAGRAWLTQVLNDAAYQHAVQPHLLPLD
                     AVTLHLPITVGDYVDYYASAHHARNVGMIFRPDSPALPINWKHLPIGYHGRSGTVVVS
                     GTPFHRPCGQRKGPQDPVPVYGPSVRLDIEAELGYVVGGATSMGERISVDEAGEHLFG
                     VVLLNDWSARDIQAWEYVPLGPYLGKSFCTSISAWVTPMAALQSARVDLPRDEADPEV
                     LPYLRGTGTGVQGSGGAAFGLDVHLEVRLNGEVVSRPEYRDMYWSPAQMLAHMTVNGA
                     SVGDGDLFGSGTISGPEKGQRGSFLELSWGGQEPFELADGSSRSYLQDGDEVAITAWA
                     PGPDGTRIDLGDVSGRVRPPR"
     gene            complement(1607..2818)
                     /locus_tag="PLK64_07805"
     CDS             complement(1607..2818)
                     /locus_tag="PLK64_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HPV79688.1"
                     /translation="MRADTSAVHAGREDLVALGVHVPPIDLSSTYPIGDLDAGGAAYE
                     TLAMGGRGEAAATLVYQRLWNPNVDRFERALAQLEGTEEAVAFASGMAALTATLIACV
                     AAGTPHIVAVRPLYGGSDHVLATGLLGTQVTWATAATVGEAITDRTGLVLLETPANPT
                     LDLVDIAAVVAQAGRVPVMVDNTFATPVLQQPRRQGATLVLHSATKFIGGHGDLLGGV
                     IAASPEWAVRLRQVRALTGALLYPLAAYDLHRGLQTLPVRVRAQQDNAIALAGWLAGH
                     PAVERVHYPGMPGADPEGLLGRQLAGPGAVLAIALRGGFDAAADLVTRTRLVTHAVSL
                     GGVDSLLQHPASLTHRPVAAEAKPHPNVVRISVGLEDVRDVIADFDQALRGVGERRLV
                     DVLSGADALAR"
     gene            2928..3458
                     /locus_tag="PLK64_07810"
     CDS             2928..3458
                     /locus_tag="PLK64_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HPV79689.1"
                     /translation="MPNNPRALPPGPAPRTPAPVDDVDRAIVDALVADARVPNVQLSR
                     AVGLAESTCIGRVRSLLARGILRGFTARVDRGKLGYPIEAMVAVRLAGTDRRRVDEFG
                     EHVSRLPGVLAAYNVSGADDFLVHLVAQSPDTLRGVVLDHLSGHPGVVHVQTSLIFRT
                     HEGRHSLPAPGPVATN"
     gene            complement(3480..4361)
                     /locus_tag="PLK64_07815"
     CDS             complement(3480..4361)
                     /locus_tag="PLK64_07815"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPV79690.1"
                     /translation="MTQPSGWYDDPSDHSRLRYWDGILWTDRTVPKQSPTAADSTIGR
                     AVDPYAASSHHAPTGPGPGPYPPAAPTYGQPPTPYGQAPTPYGQPPAGRDYGAAPTYQ
                     WRPSGPTTPDGVPLAQWWQRLVAAVIDNIIVQLASLVLAFPWWRDFLVWYLDFLQTAT
                     VSSGSVDPLTLFGEINTEMARYIVPIVLIQSAVTFVYHVLFLHRNGASVGKLALGIRV
                     RRFDRPGPLTLLEASRRQALQLVFGLVGLVPFIGMVTGVVGYVDVAWLLWDQRRQCLH
                     DKIADTLVEQKPPTRRV"
     gene            4599..5294
                     /locus_tag="PLK64_07820"
     CDS             4599..5294
                     /locus_tag="PLK64_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPV79691.1"
                     /translation="MPTSHSPEAFQAHLERVAGAPLPDFHHFQRAMSWAVIEPRELLF
                     DAGTTDRRFYFIVDGIVSLATSDRPGRDWIRRFVGPGELLSSVPALAHPLPRAAAAFV
                     GLDPDSADFGPSAYMHYSARGITRVRARYVDISTFWALSNRYREWQQLALIASIASTA
                     LQERRERELLTLNAEERYRLLVQERPELVSLVPQKDLARYIGVTPVAMSRIVSRVRQG
                     LVAEGPSRAAVSG"
     gene            5331..6650
                     /locus_tag="PLK64_07825"
     CDS             5331..6650
                     /locus_tag="PLK64_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPV79692.1"
                     /translation="MTDHAEKSTYAPRPQTRIAHPQWARAATIYQLNQRQFTHEGTFQ
                     AAREHLPRLRRLGVDIIWLMPVNPIGEERRKGGLGSPYAVRDYRAVNPEFGSLRDLRS
                     FVAAAHALGMKVILDWVANHTSWDNVLVAEHPEWYARDWKGDFRPTPWWDWDDVIDLD
                     YSHAGLRRYMTESMCYWVREADVDGFRCDVAGFVPLDFWEQARAELDAIKPVFMLAEW
                     EARDLHEAAFDMTYGWSWNETMHHIAVGRSDVTALHIFYAWNESSWPRDAIRMMFVSN
                     HDKNSWEGTEFEQFGPMRESAIVLSVIGEGMPLIYNGQEAGFDKRLAFFDKDEIIWRE
                     DPQGELYRTLFALKKAHPALWNGAAGGRMVKVPNDMETSVLTFVRTQGEDSVFAVFNF
                     TGVHRSVMLGRGPQDGPWVDAIDGSPLRGREGMRIDLAAWGWRVFVR"
     gene            6723..8105
                     /locus_tag="PLK64_07830"
     CDS             6723..8105
                     /locus_tag="PLK64_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HPV79693.1"
                     /translation="MTEAPFRTVFEPFRIHSVEPLRMTTADYRRGKVREAAYNLFQLH
                     ADDVLIDLLTDSGTGAMSRDQWAAIQHGDESYAGSPSYFIFRDAVQELFPYAHVIPTH
                     QGRAAERILFQALAGPGKVIPSNTHFDTTRANIEYTGAEAVDLVIAEGRDPAALHPFK
                     GNIDLDALETILCERGSDIPCVMMTITNNSGGGQPVSLANLRAAAEIAHRHGKPLFLD
                     AARFAENAWFIHEREPGQGERDVRDIIRDIAACADGMTMSAKKDPMGNIGGWLALNDD
                     EIAGKCRNLLILTEGFPTYGGLAGRDLEALAQGLKEVVSHDFLRYRIRSTAYVADALE
                     ALGMPCIKPAGGHAVYIDAKALLPHIPPLQFPGQALAVALYEHGGIRTCEIGSAMFGR
                     HPDGSESPAAMELVRLAIPRRTYTQSHMDYVIEVAERVMAVKDSLPGYQIVVEPPQLR
                     HFTAEFAPLG"
     gene            complement(8118..8969)
                     /locus_tag="PLK64_07835"
     CDS             complement(8118..8969)
                     /locus_tag="PLK64_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HPV79694.1"
                     /translation="MSIIEGTKQAATDRLRVIAGPAYRRVKREIQWRRCGADLALERG
                     PVSGFPWVVMAHETPLIRPLSGLEHRLQVNKVTNLRLAIEPLDGRVLQPGQRLSVWWF
                     VRAPTARRGFLEGLVLSQGRLTAGVGGGLCQLTNLIHWMTIHTPLTVVERWRHTYDVF
                     PDAGRTQPFASGATCSWPSLDLQIENRTDTAYRLGLRLTDTHLVGEWRAAVEPTRSYE
                     VYEAAHEIATDLPGRFVRRNLLRRKVFDMAGEQIDDEFVAENHALMMYPPFLSDRPTT
                     PPGPSPE"
     gene            complement(9033..9359)
                     /locus_tag="PLK64_07840"
     CDS             complement(9033..9359)
                     /locus_tag="PLK64_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018776325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3039 domain-containing protein"
                     /protein_id="HPV79695.1"
                     /translation="MPRASSMPPDPFEPQPMPSEPGTGTRTSVLEREETVVEPQVQEP
                     GDHERFSHYAHKDKIMEAMVMGTPCVALCGKVWIPSRDPKKFPVCPTCKAIFEDDSAG
                     WVPEDE"
BASE COUNT         1488 a         3336 c         3197 g         1475 t
ORIGIN      
        1 ccgcacgggt gccaccgagg tctgggcctg gcccgacgag gtcgagttgg cccggttctt
       61 caccgcctgc cgtgagcgcg agctgccgtt caagctcacc ggcggcctgc accacgtgct
      121 gcgagccgac cacccgggcc ccgatggcgg ccgccagcac ggcctgctca acgtgctcgc
      181 agcggtgcac gcgaccgccg agggcgtgcc cgaacccgag gtagccgagt tgctgggcga
      241 gcgcgacgct gccccgctcg tcgacctgct caccgccctc agcaccgacg cggccagtcg
      301 cgtgcggtgc gcgttcacgg catacggatg ctgcaccgtc gcggacccgc tcggcgagct
      361 tgcggcgttg cgtctcatcg acaaggagca ccaatgacct ggctagacct ccccgccgac
      421 caccccttcg ggcttgccca cctcccctac ggagtcttct ccacggtcgc tgacccagcc
      481 ccgcgcgtcg gggtccgcat cggcgacctc gtcctggacg ccgcgcgcgc cgcggccgcg
      541 acggggtatg ccgcgggagc cgcgtgggcg acgccctcgc tcaacgcgtt cctcgggcag
      601 ggcacgacgg cgtgggccgc ggggcgcgcc tggctgaccc aggtgctcaa cgatgcggcc
      661 taccagcacg ctgtgcagcc acacctgctc ccgctggacg cggtgaccct gcacctgccg
      721 atcaccgtcg gtgactacgt cgactactac gcgagcgccc accacgcgcg caacgtcggc
      781 atgatcttcc gacccgactc accggccctt ccgatcaact ggaagcacct gcccatcggc
      841 taccacggcc gatcgggcac ggtggtcgtg tccggcaccc ccttccaccg tccctgcggc
      901 cagcgcaagg gtccccagga cccggtgccc gtctatggcc cgagcgtccg gctcgacatc
      961 gaggccgagc tcggttacgt cgtcggcggg gcgaccagca tgggcgagcg gatcagcgtc
     1021 gacgaagccg gcgagcacct cttcggggtc gtcctgctca acgactggtc ggcccgcgac
     1081 atccaggcgt gggagtacgt cccgctcggc ccctacctcg gcaagagctt ctgcacgtcg
     1141 atctcggcgt gggtgacccc gatggcggcc ctgcagtccg cccgggtcga cctgccgcgg
     1201 gacgaggccg atcccgaggt cctgccctac ctgcgcggca ccggcaccgg cgtgcagggc
     1261 tcgggtggcg ccgccttcgg gttggacgtg cacctcgagg tgcgcctcaa cggcgaggtg
     1321 gtcagccggc cggaataccg cgacatgtat tggtcccccg cccagatgct cgcccacatg
     1381 accgtcaacg gtgccagcgt gggcgacggc gacctgttcg gctcgggcac gatcagcggc
     1441 cccgagaagg ggcagcgcgg gtccttcctt gagctgtcct ggggtggcca ggagcctttc
     1501 gagttggccg acgggtcgtc gcgcagctac ctgcaggacg gtgacgaggt cgccatcacc
     1561 gcctgggcac cggggccgga cggcacccgg atcgacctcg gtgacgtcag cgggcgagtg
     1621 cgtccgcccc gctgagaacg tcgaccaacc gacgctcccc gaccccgcgc agggcctggt
     1681 cgaagtcggc gatgacgtca cggacgtcct ccaagccgac cgagatccgc acgacgttgg
     1741 ggtgcggctt ggcctcggcg gcgaccgggc gatgggtgag gctggcgggg tgctgcagca
     1801 ggctgtcgac tccgccgagg ctcacggcgt gggtcaccag ccgagtccgc gtgacgaggt
     1861 cggcagccgc gtcgaagcca ccgcgcagcg cgatcgccaa cacggctccg gggccggcca
     1921 actgccgacc cagcaggccc tcggggtccg cacccggcat accggggtag tggacgcgct
     1981 cgacggccgg gtgacctgcg agccagccag cgagggcgat ggcgttgtcc tgttgggcgc
     2041 gcacccgcac ggggagggtc tgcaggccgc ggtgcaggtc gtatgccgcg agcggataca
     2101 gcagcgcgcc ggtcaacgcg cgcacctgcc gaaggcgcac ggcccattcg gggctggccg
     2161 cgatgacgcc accgagcagg tcaccgtgcc ccccgatgaa cttcgtcgcc gagtgcagca
     2221 cgagggtcgc gccctggcga cgcggttgct gcaggacggg cgttgcgaag gtgttgtcga
     2281 ccatgaccgg cacacgaccg gcttgggcga cgacggcagc gatatccacc aggtcgaggg
     2341 tcgggttggc aggggtctcc aacagcacca acccggtccg atcggtgatc gcctcaccga
     2401 cggtcgcggc cgtggcccag gtgacctggg tgccgagcag gccagtggcc aggacgtgat
     2461 ctgacccgcc gtagagcggg cgcaccgcga cgatgtgcgg cgtgccggcg gcgacgcagg
     2521 cgatgagggt ggcggtcagc gctgccatcc cggaggcgaa ggcgacggcc tcctcggtgc
     2581 cttccaactg cgccaaggcc cgctcgaacc ggtcgacgtt ggggttccag agccgctgat
     2641 agacgagggt cgccgcggct tcaccgcgtc cacccatggc gagcgtctcg tatgccgcgc
     2701 cgccggcgtc gaggtcgccg atcgggtagg tgctcgacag gtcgataggc gggacgtgca
     2761 cgccgagggc gaccaggtct tcgcgaccgg cgtggacggc ggaggtgtct gcgcgcatac
     2821 ctccagtgtc gacaatccac ggatcaagct ccatagaccc gtggatcctc gccttcttag
     2881 gccgcgatgc cgcagattcc tcggccatgg acgtttacag tggccctgtg ccgaacaacc
     2941 cacgcgcgtt gcctccaggg ccagcgcccc gcacgccggc gcccgtcgac gatgtcgacc
     3001 gagcgatcgt ggatgccctc gtcgccgatg cccgggtccc caatgtccaa ctctcgaggg
     3061 ccgtcgggct ggcggagtcg acctgcatcg gtcgcgtccg gtcactgctg gcgcggggca
     3121 tcctgcgtgg cttcaccgct cgggtggacc gcgggaaact cggctacccc atcgaagcga
     3181 tggtcgccgt gcgcctggcc ggcaccgacc gtcgccgggt cgacgagttc ggcgagcatg
     3241 tgtcgcggct acctggcgtg ctggcggcat acaacgtcag cggcgccgac gacttcctcg
     3301 tccatctcgt cgcgcagtca ccagacaccc tgcgcggcgt ggtccttgac cacctgtcag
     3361 ggcatcctgg cgtcgtccac gtccagacgt cgctcatctt ccgcacgcac gagggacggc
     3421 acagcctccc cgcgccgggt ccggtcgcga cgaactaacc gtgcctgggc gaccggccgt
     3481 cacacccgcc gggtgggcgg cttctgctcc acgagggtgt cggcaatctt gtcgtgcagg
     3541 cactggcgac gctgatccca cagcagccag gccacgtcga cgtagccaac aacgcccgtc
     3601 accatgccga tgaacggaac caggccgacc aggccaaaca ccaactgcag ggcctgacga
     3661 cgggacgctt cgagcagggt gagcggccca ggtcgatcga accgtcggac ccggataccc
     3721 agagccagct tgccgacgct ggcgccgttc cggtggagga agagcacgtg atagacgaac
     3781 gtcaccgctg actggatcag gacgatcggg acgatatagc gagccatttc ggtgttgatc
     3841 tcgccgaata gtgtcagcgg gtcgaccgac cctgatgaca cggtcgccgt ctgcaggaag
     3901 tcgaggtacc acaccaggaa gtcacgccac caggggaacg ccagcacgag actcgcgagc
     3961 tgcacgatga tgttgtcgat cacggcggcc accagtcgct gccaccactg ggccaggggc
     4021 accccgtcgg gggtcgtcgg accggacggg cgccattgat acgtcggcgc tgctccgtag
     4081 tcgcgacccg ccggcggctg gccgtacgga gtcggagcct ggccgtaggg agttggtggc
     4141 tgcccgtagg tcggagccgc cggtgggtag gggccgggac cgggaccggt gggtgcgtgg
     4201 tggctcgacg cggcatacgg gtcgaccgcc cgcccgatcg tcgagtcggc ggccgtcgga
     4261 gattgcttcg gcacggtccg atccgtccag aggatgccgt cccaatagcg caaacggctg
     4321 tgatccgacg ggtcgtcgta ccacccggaa ggctgcgtca tgcccctacc ttcgcacgcc
     4381 gagccgctcg tcccgtccac acggaccatg acccaccgag aactcccgag tatctgggga
     4441 tcttgttggt ccctttcctc ccaaacaggc agcgcccgtc gtagactccc ccccggggat
     4501 tcgtcagcgg gatcgaaggg gacacgtgta ccggccactt ggccatgctc tgcgcggctc
     4561 ggtttccgtt caggcaatca tcaagggaga ggcgcgcggt gcccacctcg catagtccgg
     4621 aagccttcca ggctcacctg gagcgagtcg cgggggcccc gctgccggac ttccaccact
     4681 tccagcgggc gatgtcctgg gcggtgatcg agccccgtga gttgctcttc gacgcaggca
     4741 cgacggaccg acgcttctac ttcatcgtcg acggcatcgt cagcctggcg acctccgatc
     4801 gcccgggccg agactggatc cgtcgattcg tcgggccggg cgagttgctc tcctcggtgc
     4861 ccgcgttggc ccaccccctc ccccgcgcgg cggcggcctt cgtcggcctg gaccccgaca
     4921 gtgcggactt cggtccgtcg gcgtacatgc actacagcgc ccgcggcatc acccgggtcc
     4981 gtgcgaggta cgtcgacatc tcgaccttct gggcgctgtc gaaccgctat cgcgaatggc
     5041 aacagttggc cctcatcgcc tcgatcgcct cgaccgccct tcaggagagg cgcgagcgcg
     5101 agttgctcac cctcaacgcg gaggagcgct accggctcct tgtccaggag cggcccgaat
     5161 tggtcagcct cgtgccgcag aaggacctcg cgcgctacat cggcgtgacc ccggtggcga
     5221 tgagccgcat cgtgtcccgg gtccggcagg gcctggtggc ggagggcccc agccgggcgg
     5281 ccgtgagcgg gtaggcggga cccgtcgcga gcggcatacg cttagggccc gtgaccgacc
     5341 acgccgaaaa gtccacgtat gccccgcggc cgcagacgcg gatcgcgcat ccgcagtggg
     5401 ctcgcgcggc caccatctac caactcaacc aacggcagtt cacccacgag ggcaccttcc
     5461 aggcggctcg cgaacacctc ccccggctgc gccggttggg cgtggacatc atctggctga
     5521 tgccggtcaa ccccatcggc gaggaacggc gcaagggggg cctgggcagt ccgtatgccg
     5581 tgcgcgacta ccgcgcggtc aaccccgagt tcggctcgct gcgcgatctg cgctccttcg
     5641 tcgcggcggc gcacgccttg gggatgaagg tcatcctcga ctgggtggcc aaccacacgt
     5701 catgggacaa cgtgctggtc gcggagcacc ccgagtggta cgcgcgggac tggaagggcg
     5761 acttccggcc gactccgtgg tgggactggg acgacgtcat cgatctcgac tactcacatg
     5821 cggggctgcg ccgctacatg accgagtcga tgtgctactg ggtgcgggaa gccgacgtcg
     5881 acggattccg ctgcgacgta gccggtttcg tcccgctcga cttctgggag caggcacgcg
     5941 ccgagctcga cgcgatcaag cccgtcttca tgctcgccga atgggaagcc cgagacctcc
     6001 acgaggccgc cttcgacatg acctacggct ggtcctggaa cgaaacgatg catcacatcg
     6061 ccgtgggccg atcggacgtc accgccctgc acatcttcta cgcctggaac gagtcgtcct
     6121 ggccgcgcga cgccatccgg atgatgttcg tctccaacca cgacaagaac tcctgggagg
     6181 gcaccgagtt cgagcagttc gggccgatgc gcgagtccgc cattgtgttg tcggtcatcg
     6241 gcgaaggtat gccgctcatc tacaacggtc aggaggccgg cttcgacaag cgcctggcct
     6301 tcttcgacaa ggatgagatc atctggcggg aggaccccca gggcgagttg taccgcacgc
     6361 tgtttgccct caagaaggct cacccggcgc tgtggaacgg tgccgcaggt ggccgcatgg
     6421 tcaaggtccc caacgacatg gagacctcgg tgctgacctt cgtgcgcact cagggcgagg
     6481 acagcgtctt cgcggtgttc aacttcacgg gagtgcaccg cagcgtgatg ctgggtcggg
     6541 gcccccagga cggcccctgg gtcgacgcca tcgacggctc acccctgcgg ggccgcgagg
     6601 ggatgaggat cgatctggcg gcctggggtt ggcgcgtgtt cgtgcgctga accgagcctg
     6661 gcgaaagaaa cttcggcgtc gggactgacg aaggacgatt tcggcggcat acagtctcgt
     6721 tcgtgactga agccccgttc cggaccgtgt tcgagccctt ccgcatccac tcggtggagc
     6781 cattgcggat gaccaccgcc gactatcgcc gcgggaaggt gcgggaggcg gcatacaacc
     6841 tgttccaact gcatgccgat gacgtcctca tcgacctgtt gaccgactcg gggaccgggg
     6901 cgatgagccg cgaccagtgg gccgcgatcc agcacggcga cgagtcctac gccggctcgc
     6961 cgtcctactt catcttccgt gacgcggtcc aggaactgtt cccctacgcg cacgtcatcc
     7021 ccacgcacca ggggcgcgcc gccgagcgga tcctcttcca ggcgctggcc gggcccggca
     7081 aggtcatccc gtcgaacacc cacttcgaca cgacccgcgc caacatcgaa tacaccggcg
     7141 ccgaagccgt cgacctggtg atcgccgagg gccgcgaccc cgcagcgctg catcccttca
     7201 agggcaacat cgatctcgac gccctggaaa ccattctgtg cgaacgcggt tcggacatcc
     7261 cctgcgtgat gatgacgatc accaacaact ccggcggagg ccaaccggtc tcgctggcca
     7321 acctgcgggc cgccgccgag atcgcccatc gacacggcaa gccgctcttc ctcgacgcgg
     7381 ccaggttcgc cgagaacgcc tggttcatcc acgagcgcga gcccggtcag ggtgagcgcg
     7441 acgtgcgcga catcatccgc gacatcgcgg cctgcgccga cggcatgacg atgtcggcca
     7501 agaaggaccc gatgggcaac atcggcggtt ggctcgcgct caacgacgac gaaatcgccg
     7561 gcaagtgccg caacctgctc atcctcaccg aaggcttccc gacctacggc ggtctggccg
     7621 gccgcgacct ggaggcgctc gcgcaggggc tcaaggaggt cgtctcgcac gacttcctgc
     7681 gctaccggat ccgctcgacc gcttacgtcg cggatgccct ggaggcattg ggtatgccgt
     7741 gcatcaagcc cgcgggcggg catgcggtct acatcgacgc caaggccctg ctcccccaca
     7801 tcccgccgct gcagttcccc ggccaggcgc tggccgtcgc gctctatgaa cacggtggca
     7861 tccggacctg cgagatcggg tcggcgatgt tcggtcgcca ccccgacggc tccgagtcgc
     7921 cggcggcgat ggagttggtc cgcttggcga tcccccggcg cacctacacc cagagtcaca
     7981 tggactacgt catcgaagtg gccgagcggg tcatggcggt caaggactcg ttgcccggct
     8041 accagatcgt tgtcgaaccg ccgcagttgc ggcacttcac cgcggagttc gccccactcg
     8101 ggtgagccgg gggccggtca ttcgggcgat ggccccggtg gcgtggtcgg ccggtcgctg
     8161 aggaacggtg gatacatcat cagcgcatgg ttctcggcga cgaactcgtc gtcgatctgc
     8221 tcccccgcca tgtcgaacac cttgcgccgc agcaggtttc gccggacgaa ccgcccgggc
     8281 agatcggtcg cgatctcgtg ggccgcctcg tagacctcgt agcttctggt gggttccacc
     8341 gctgcccgcc actccccgac caggtgcgtg tcggtcagcc gcaaccccag ccggtatgcc
     8401 gtgtccgtcc ggttctcgat ctgcaggtca agcgacggcc atgagcaggt cgcgccgctg
     8461 gcaaacggct gggtccgccc ggcgtccggg aagacgtcat aggtatgccg ccatcgctcc
     8521 accaccgtca gcggggtgtg aattgtcatc caatggatga ggttggtgag ttggcacagg
     8581 ccgccgccga cccctgcggt gagtcgtccc tggctgagca ccagtccttc gaggaagcct
     8641 cggcgggccg tcggcgcccg cacaaaccac cagaccgaga gccgctggcc cggctgcagc
     8701 actcgcccat cgaggggttc gatggccagg cgcaggttag tcaccttgtt gacctgcagt
     8761 cggtgctcaa gcccggacag gggccggatg agcggggtct cgtgcgccat cacgacccag
     8821 gggaaccctg agacaggccc ccgctccaag gccaggtcgg cgccgcaccg acgccactgg
     8881 atctctcgct tgacccgccg gtaggccggc ccggcgatga cgcgcaggcg atccgtcgcg
     8941 gcctgcttgg tgccctcgat gatcgacatg tcgtcccctt ccgacctctc ccactttaag
     9001 caggagaagg ctgcggcgac agcgcgagtg ggctattcgt cctcggggac ccagccggcg
     9061 ctgtcgtcct cgaagatcgc cttgcacgtc gggcacaccg ggaacttctt ggggtcgcgg
     9121 ctggggatcc agaccttgcc gcacagcgcg acgcagggcg tgcccatgac catggcttcc
     9181 atgatcttgt ccttgtgggc gtagtggctg aaccgctcgt ggtcccccgg ctcctgcacc
     9241 tggggctcaa cgacggtctc ctcgcgctcc agcacgctgg tgcgcgtgcc ggtgcccggc
     9301 tcggacggca tcggttgggg ctcgaacggg tcgggcggca tcgaggaggc acgcggcata
     9361 ccgacaaccg taaccgaacc acgacgccag gcgcccctgt ggaaatcgcc cgaggagtgt
     9421 cggcgacatc gaggagactc gcgcctatga ccgtaaggag gcaccatggg tctcatgacc
     9481 gtcctggccc ggaccg
//
LOCUS       DAOMZY010000112         9490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_6840_length_9490_cov_4.706412, whole genome shotgun sequence.
ACCESSION   DAOMZY010000112 DAOMZY010000000
VERSION     DAOMZY010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9490
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6840_length_9490_cov_4.706412"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PLK64_07845"
     CDS             <1..204
                     /locus_tag="PLK64_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79696.1"
                     /translation="TAVSAGREGPLLLREGDEVVCFNADGLLLTGPGQPRRLIHGDLV
                     TIRCEPGPWWLEDGRRVVHALTH"
     gene            complement(247..3069)
                     /locus_tag="PLK64_07850"
     CDS             complement(247..3069)
                     /locus_tag="PLK64_07850"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79697.1"
                     /translation="MTDDAEPITTVWLVPHTHWDREWYEPFQRFRLRLVDLMDDVVDR
                     ALAEPEFRFTMDGQMAAVDDYLEVRPERTEAIRRLVAHGQLAIGPWRVLMDEFLCSGE
                     TMIRNLEIGLADAARLGPSMPVGYLPDMFGHCAQMPQLLLGVGIERACVWRGVPAGVK
                     RSEFAWRSPDGSTIRTEYLANSYGNAADLFADTEGTAGRLLARIRTQSQWYAAGGFLA
                     MYGTDHAAPLPGLLDQVAALNASDGPEVRVATLTEYLDAIPAPVDSPVVPGELRSHAR
                     ANILPGVLSVRWPLKEAMAVSERLLARYAEPLAALVLREVPQGYLDLAWRRVVDASCH
                     DSVTGCGVDETALQVQARLEEAGHLAQAVRDRALDLLAQASPAGSVVVVNPLPTPRDD
                     LIELTLPVPSQWPAVELVSSTGQVVATQELSRPEPVLARETVASADLQRLIHRIHDRE
                     LFGLQLTGVDIDADERRVTFALAEAGDPGFDSVATGDRLAQAAARHTPQAPWEVVTRA
                     APTRTLAAKVPVPALGWASFQVRPGQAVAEPSNAASPLVPGGSVLPILCADRTLDNGL
                     IRVEVHDDGTFSVRGADGSFATGLGRIVDGGDCGDAYNYGPPGVDALVTTPLSVTVES
                     GGSRGPVLGRLTVTRDYALPATSDHGGRSAEVVATPVVLHLEVRVDEPFVRVAVSWEN
                     RTRDHRLRLHLPTPRTASGSHAQGQLAVVSRSRHPESGPVGEYPLATFPAERFIDAGG
                     LGAILTRTTEYEITDGPDGTEIALTLLRAVGWLSRNVHPHRNEPAGPQLPTPAAHVIG
                     PGSLNLAVLPHDGSWAQAGLGRWAESMLHPVVVLRGGASEGSETDSVEGLTIDGKEVD
                     LLSLRRREDGWLQVRVVNSCDEPDTLRLSSAVRPVLAAVRCDLLGRHGEVLDVIDEGA
                     VLVPVRPWEIATIALDVGPGPLLHKQ"
     gene            complement(3066..4184)
                     /locus_tag="PLK64_07855"
     CDS             complement(3066..4184)
                     /locus_tag="PLK64_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HPV79698.1"
                     /translation="MVVITEDFRGDDAPKDDGRRDDRVKDERGEAAPSLTLQSLSVDV
                     LVVGVTYLDVVVADLDEFPGPGEEARAGGLTLCPGGIATRAVATARLGLDTVLSTSLG
                     TDRAGALVRAALGQEAVRLDLADDLAGASTPVTVSVAHDGDRRMFTRDSLAPVRQPRM
                     LLDDGMCRCIMVDLRAGQDDVFPLLVDVWTAGAYVVADVGWDPSGIWDPAVLKPLSVC
                     DLFTPNAVEAMAYTGADTPEDAARRLAEQVPLAVVTDGARGVHAASLEGTRPCEVFVP
                     AVTAYGTRQRDATGAGDVFGAALVAGTLWGLPLADRLAFGALCAALSLSSLGGAPGAP
                     GWTELQRWWSRALQQGDSPVLSRYEFLDSLLADQRCSA"
     gene            4272..5030
                     /locus_tag="PLK64_07860"
     CDS             4272..5030
                     /locus_tag="PLK64_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HPV79699.1"
                     /translation="MKYVRLRTITERLASGDSVSVLNLADELGVSPSTVRRDLQSLAA
                     SGILLRVHGGAAPVDGRDLGDRPFDLVADEDAIDKGRIAGRAATLVNDGDVVLLDIGT
                     TTRLLARELFGRPITAVTSSLAVLDELRGDTTSEVVLLGGAVRRQYHSLVGSLTEDAL
                     RQVRAATVFLSASGVSSDGAVLDTTLVEMPTKRGLLRAANRVVLLAGSSKFPGSGSFR
                     VCGMDQIDVLVTTHGADPATLKACRAQGTEVLFA"
     gene            5027..6400
                     /locus_tag="PLK64_07865"
     CDS             5027..6400
                     /locus_tag="PLK64_07865"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HPV79700.1"
                     /translation="MRLVLLGGGGFRVPLVHRALLDDEHAGRVDELVLHDVDEGRLET
                     MTRVLAQQGAGAPRPVTVRATARIDEALEGADFIFSAIRVGGLSGRTLDERIALDHAV
                     LGQETVGAGGISYGLRTVPVAVGIAERIAALAPNAWLINFTNPAGLITQAIQAVLGDR
                     AIGICDSPVGLGRRAATALGFQGADVQLGYAGLNHLGWVDSLVINGVDRLPDLLADDA
                     ALLSIEEGRLFGTEWIQSLAKLPNEYLYYYYFTREAVAAIMGEPQTRGEFLLDQQTGF
                     YQRALAAPSSAYRQWRSAWEDRETTYMREVRQPTVQRDARDFGGGYERVALALMAAIA
                     HDEPARLVLNVRNRGAVECLGVDAVVEVPCLVDAGGPVPQLSRISGGHERGLVQQVKA
                     AEELTIEAALTGSRRRAVQAFATHPLVDSVGVARDLFDAYAAAFPQLGAIFAAQPVPG
                     SPSVAGH"
     gene            6497..6570
                     /locus_tag="PLK64_07870"
     tRNA            6497..6570
                     /locus_tag="PLK64_07870"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6531..6533,aa:Val,seq:cac)
     gene            6577..7488
                     /locus_tag="PLK64_07875"
     CDS             6577..7488
                     /locus_tag="PLK64_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPV79701.1"
                     /translation="MNIPPSGAAATGSVLLGGMLFGTAGVAQAFAPSGAHPLTVGAMR
                     LSVGAIALIIFVLARGDTFGSVVRLWGSPAGLIATAGAAAYQPFFFAGVARVGVPLGT
                     LVAVGSAPIFTGLLGWVWRRERPTGTWALATTICVAGLALLTGAGAQVGSLDGIALTA
                     GAGVSIACYTVAAKSLLESGVDAIGVCASTFLLGSIVVLPFALGQGIGWALTGSGVLV
                     ALYLGLATMAVANILYARGLLHLPPATAATLGLADPVTATALGVLVLGQTVSPLGWLG
                     FLGVVVGLLLQGIWAARAGRRDQLPLG"
     gene            7586..>9490
                     /gene="thrS"
                     /locus_tag="PLK64_07880"
     CDS             7586..>9490
                     /gene="thrS"
                     /locus_tag="PLK64_07880"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPV79702.1"
                     /translation="MSASITVSVAGNERSVAEATTAADLFADDRAIVVARVNGELRDL
                     AHVLADGDAVEPVRIDDPDGLQVLRHSCAHVLAQAVQQVNPDAKLGIGPPIRDGFYYD
                     FDVTTPFTPEDLKELEKVMQRIINEGQTFVRREVSDEDALVELAGEPFKCELIGLKGG
                     SAEDAAEGAGVEVGGAQLTIYDNVRRDGTRAWGDLCRGPHIPTTKLLGNAFKVTRSSA
                     AYWRGDQANQQLQRVYGTAWPTKADLTAYLDRLAEAERRDHRKLGIELDLYSFPDELG
                     SGLPVFHPKGGVLKREMEDYARRRHIEEGFLYVGTPHISKEGLFHTSGHLPYYADTMF
                     PPMSMEGSDYYLKAMNCPMHNLIYRSRGRSYRELPLRLFEFGSVYRYEKSGVIHGLTR
                     VRGLEMDDSHSYVTPEQAPAEIKHLLNFVLSLLRDFGLDDFYLELSTRDDSGAKKGKF
                     IGSEEQWAEATQILADVAAESGLDLVPDPGGAAFYGPKISVQARDAIGRTWQMSTIQY
                     DFNQPERFGLEYQAADGSRQQPVMIHSAKFGSIERFMGVLVEHYAGAFPPWLSPVQVL
                     GVPVAEEYAGYLREVLGGLAAQGVRVELDESDDRFPKKIRNASKSKVPFVLIAGEDDR
                     AAGAVSFRFRD"
BASE COUNT         1448 a         3291 c         3299 g         1452 t
ORIGIN      
        1 acggccgtca gcgccggacg ggaggggcca ctgctgttgc gagagggcga cgaggtggtc
       61 tgtttcaacg cggacggcct cctactcacg gggcctgggc agccccggcg gctgatccat
      121 ggtgatctgg tgacgatccg gtgcgagccg gggccatggt ggctcgagga tggccgccgc
      181 gtcgtgcacg ctctgaccca ctgacgcgga ctctccgggt caccggcact gcagacacct
      241 cgaccgtcac tgcttgtgaa gaaggggtcc gggaccgacg tcgagggcga tcgtggcgat
      301 ctcccagggc cgcaccggca cgaggacggc accctcgtcg atgacgtcga gcacctcacc
      361 gtgccggccg agcaggtcgc atcggacggc cgcgagcacc gggcggacgg cggatgagag
      421 ccgcagtgtg tcgggttcgt cgcacgagtt gacgacccga acctgcagcc agccgtcctc
      481 ccggcggcgc agcgagagca ggtccacctc cttcccatca atcgtcagac cctcgaccga
      541 gtccgtctcc gacccttcgg aagcgccccc ccgaaggacg acaaccgggt ggagcatcga
      601 ctcggcccat cggccgagcc cggcctgagc ccaggacccg tcgtgcggca ggacggcaag
      661 gttcagcgac ccagggccga tgacatgagc ggccggtgtc ggcagttgcg gccccgccgg
      721 ctcgttgcgg tggggatgca cgtttctgct caaccagcca acagcgcgca ggagggttag
      781 cgcgatttcg gtgccgtccg gcccgtcagt gatctcgtat tcggtggtcc gcgtgaggat
      841 ggctccgagg cccccggcat cgatgaaccg ctcggcaggg aaggtggcca gcgggtactc
      901 gccgaccgga ccggattccg gatgccgact gcgggagacc accgcgagtt ggccctgggc
      961 gtgtgaccct gatgcggtgc gcggcgtcgg caggtgcagc cgcaggcggt ggtcacgggt
     1021 ccggttctcc caggacaccg ccacccgcac gaacggctcg tcgaccctga cctccagatg
     1081 caagacgaca ggggtggcga ccacctcggc ggaacgaccg ccatgatcac tcgtcgccgg
     1141 cagggcgtaa tcgcgcgtga ccgtcaggcg tccaagcacg ggcccgcgag accctcccga
     1201 ctcgacggtc accgacaggg gagtggtcac gagtgcatcg acgcccggcg gcccgtagtt
     1261 gtaggcgtcg ccgcagtccc caccgtcgac gatccggccc aggcccgtcg cgaacgaccc
     1321 gtccgcaccg cgcacggaga acgtcccgtc gtcatggacc tcgacccgga tgagcccgtt
     1381 gtccagggtc cggtccgcgc acaggatggg gagaaccgac ccgcccggca ccagcggact
     1441 ggccgcgttg gatggttcgg ccacggcctg gcccggccgg acctggaagc tcgcccaacc
     1501 cagcgccggc acggggactt tcgcggccag ggtccgcgta ggtgcggccc gagtgacgac
     1561 ctcccacggc gcctgcggag tatgccgcgc agccgcctgt gcgagtcggt cgcccgtggc
     1621 gacgctgtcg aaccctgggt ctccggcttc cgcgagcgcg aacgtcaccc gtcgctcgtc
     1681 ggcgtcgatg tcgacacccg tcagttgcag gccgaacagt tcacgatcgt ggatgcggtg
     1741 gatcaggcgc tgcaggtccg ccgatgcgac ggtctctcgc gccagcaccg gctccggccg
     1801 agacaactcc tgggtggcca cgacctggcc cgtcgacgag accaactcca ctgcgggcca
     1861 ctgcgaagga accggcaagg tcagctcgat caggtcatcc cgaggcgtcg gcagcgggtt
     1921 gacgacgacg acggagcccg cggggctggc ctgggccagc aggtccaggg cgcggtcgcg
     1981 aacggcctgg gcgaggtgcc cggcctcctc gagtcgggcc tgcacctgaa gggcggtctc
     2041 gtcgaccccg caaccggtga ccgagtcatg gcacgacgca tcgaccaccc gtcgccaggc
     2101 cagatcgagg taaccctggg ggacctcccg aagcaccaag gccgccagcg gctcggcata
     2161 ccgggcaagc agtcgctcgc tcaccgccat ggcctccttg agtggccacc gcacggagag
     2221 gacgccgggc aggatgttgg cacgggcgtg actgcgcaac tcaccaggga cgacgggtga
     2281 gtcgaccggc gcggggatgg cgtcgaggta ttcggtgagc gtggcgaccc ggacctccgg
     2341 cccgtccgac gcgttgaggg ccgccacttg gtcgagcagg cctgggagtg gtgcggcatg
     2401 gtcggtgccg tacatcgcga ggaagccgcc cgcggcatac cactgcgatt gggtcctgat
     2461 cctggcgagg agccgacccg cggtcccctc ggtgtcggcg aagaggtctg cggcgtttcc
     2521 gtagctgttg gctaggtatt cggtgcgaat ggtcgagccg tcgggggagc gccaggcgaa
     2581 ctcgctgcgc ttgacaccag ccgggacgcc gcgccagacg caggcgcgct cgatgcccac
     2641 gccgagcagc agttggggca tctgggcgca gtgcccgaac atgtccggga gatatcccac
     2701 aggcatgctc gggccgaggc gcgcagcgtc ggcgagcccg atctcaaggt ttcggatcat
     2761 ggtctcgccc gagcagagga actcgtccat gaggacccgc caggggccga tcgccaactg
     2821 accatgggcg accaggcgac gaatcgcctc ggtccgctcc ggtcggacct cgaggtagtc
     2881 gtcgacggcc gccatctgtc cgtccatcgt gaaccggaac tcgggctcgg cgagggctcg
     2941 gtcgacgacg tcgtccatga ggtcgaccag acgcaggcgg aatcgctgga agggctcgta
     3001 ccactcgcgg tcccagtggg tgtgggggac gagccagacc gtcgtgatcg gctccgcgtc
     3061 gtccgtcacg cggagcatct ctggtcggcc agaaggctgt cgaggaattc gtagcgggag
     3121 agcacggggg agtctccctg ttgcagagcg cgcgaccacc agcgctggag ctccgtccac
     3181 cccggagccc ccggcgcacc accgagggag gacagcgaca gggcggcgca gagggccccg
     3241 aacgccagcc ggtcagccag agggagaccc cacaacgtcc ctgccaccag cgcggcgccg
     3301 aacacgtcgc cggctcccgt ggcgtcgcgt tggcgcgtcc cgtatgccgt gacagccggc
     3361 acgaacacct cgcacggccg ggtgccttcc agcgacgcgg cgtgcacgcc ccgggccccg
     3421 tccgtgacga cggcgagggg gacctgctcg gcgagccgcc gcgccgcgtc ttcgggggtg
     3481 tccgcgccgg tgtaggccat cgcctccacg gcgttggggg tgaagaggtc gcagacgctc
     3541 aagggcttga gcaccgccgg atcccagatg cccgaagggt cccatccgac gtcggcgacg
     3601 acgtaggccc cagcggtcca gacgtcgacc aggagcggga agacgtcgtc ctgaccggcg
     3661 cggaggtcaa ccatgatgca gcggcacatc ccgtcgtcga gcagcatccg aggttgcctg
     3721 acgggagcga gggagtccct agtgaacatc cgacgatcac cgtcgtgagc gacggagacg
     3781 gtgaccggag tgctcgctcc cgccaggtcg tcggcgaggt caaggcgcac ggcctcctga
     3841 ccgagggcgg cccgcacgag ggcccccgcg cggtcggtgc cgagggaagt ggacaggacg
     3901 gtgtccagcc cgaggcgggc ggtggcgacc gcccgggtcg cgatccctcc cggacacagc
     3961 gtgaggccgc cggcccgggc ctcctcgccc gggccgggga actcgtcgag gtcggcgacg
     4021 acgacgtcga ggtaggtcac cccgacgacc aggacgtcga ccgacaggct ctggagcgtc
     4081 agcgagggag cagcctcgcc ccgctcgtct ttgactcggt cgtctcgtcg gccgtcgtcc
     4141 ttgggggcgt cgtcgcctcg gaagtcctcg gttatcacga ccatcttcgg ggccctctca
     4201 cggtggaacc agtcgcgcgt tattgcgtat tattgcgcac ttcactacag tatggctatg
     4261 ggagcggacc gatgaaatac gtgcgactgc gcacgatcac cgaacggctg gcatcgggtg
     4321 acagcgtctc ggtcctcaac ctcgctgacg aattgggggt cagcccctcc acggtgcggc
     4381 gcgatctgca gtccttggcg gcctcgggca tcctcctgcg ggtccatggt ggtgcggctc
     4441 ccgtcgatgg tcgtgacctg ggcgaccgcc cgttcgacct ggtcgccgac gaggacgcca
     4501 tcgacaaggg gcggatcgct ggtcgggcgg ccacgctcgt caacgacgga gacgtggtgc
     4561 tgctggacat cggcacgacg acccggctgt tggcccgtga gctcttcggg cgaccgatca
     4621 ccgcggtcac gtcgagtctg gccgttctcg acgaacttcg aggtgacacg acgtcggagg
     4681 tcgtcctgct gggcggagcg gtccggcgcc aataccactc gctggtgggc agccttaccg
     4741 aagacgccct ccgccaggtc cgcgccgcca cggtgttcct cagcgcgagc ggcgtcagtt
     4801 cggacggggc ggtgctggac accacgttgg tcgagatgcc gaccaagcgc ggcctcctcc
     4861 gtgcggccaa ccgggtcgtc ctgctggctg ggtccagcaa gttccccgga tccggttcct
     4921 tccgcgtgtg cgggatggat cagatcgacg tcctcgtcac cacccacgga gccgatcccg
     4981 cgacgctgaa ggcctgtcga gcccagggga cagaggtcct cttcgcatga ggctcgtcct
     5041 cctcggcggc ggtggattca gggtccccct ggtgcaccgg gcgctgttgg acgacgaaca
     5101 cgccgggcgg gtggacgaac tcgtcctgca cgacgtcgac gaggggcgcc tcgagacgat
     5161 gaccagggtg ctcgctcagc agggagccgg cgcgcctagg ccagtgaccg tccgcgcgac
     5221 tgcccgcatc gacgaggccc tcgaaggcgc tgacttcatc ttctcggcca tccgggtcgg
     5281 cgggctgagc ggccggacgc tcgacgagcg gatcgccctc gatcacgcag tgctgggcca
     5341 ggagacggtc ggcgccggcg gcatcagtta tggactgcga acggtgccgg tggcggtggg
     5401 catcgccgag cggatcgcgg ctctcgcgcc gaacgcctgg ctcatcaact tcaccaaccc
     5461 tgcgggcctc atcacccagg cgatccaagc cgttctcggt gatcgcgcga tcgggatctg
     5521 cgactcgccg gtcggcctcg gccgccgcgc cgccacagcg ctgggtttcc agggtgccga
     5581 cgtccaactg gggtatgccg gtctgaacca cctggggtgg gtggactcgc tcgtcatcaa
     5641 cggcgtcgac cgactcccgg acctcctcgc cgacgacgcc gccctgctgt cgatcgagga
     5701 gggtcggctg ttcggcaccg agtggatcca gtccctggcc aagctgccca acgagtacct
     5761 ctactactac tacttcaccc gagaggccgt cgcggccatc atgggcgagc cacagactcg
     5821 gggcgagttc ctcctcgatc agcagaccgg gttctaccag cgtgccctcg cggccccttc
     5881 cagcgcgtac cgccaatggc gctcggcctg ggaggaccgc gagacgacct acatgcgtga
     5941 ggtgcgccag cccaccgtcc agcgggacgc gcgggacttc gggggaggct acgaacgggt
     6001 cgccctcgcg ctgatggccg cgatcgcgca cgacgaaccc gctcgactgg tcctcaacgt
     6061 gaggaatcgt ggcgccgtgg agtgcctcgg ggtcgacgcc gtcgtggaag tgccctgtct
     6121 ggtggatgcc ggcggcccgg tgccccagct cagtcggatc agcggtggcc acgagcgcgg
     6181 actcgtccaa caggtcaagg cggcggagga gttgacgatc gaggcggccc tcaccgggtc
     6241 caggcgccgc gcggtccaag cgttcgccac ccatccgctc gtcgactcag tcggggtcgc
     6301 ccgggacctc ttcgacgcct acgcggcagc ctttccgcag ttaggtgcca tcttcgccgc
     6361 tcaacccgtc cctgggtcac cctccgtcgc gggtcactga ggcgaggcgg cataccccgg
     6421 tcggggtggt gattggcact cgaccggacc cgcccgctat ccttgccact cgcggccggc
     6481 gaccctggcc gcatctggat gtgtagctca gttggttaga gcgttcgctt cacacgcgag
     6541 aggtcagggg ttcgagtccc ttcacatcca cccttggtga atatcccccc atcgggcgct
     6601 gccgcaaccg gatccgtcct gctgggcggg atgttgttcg gcacggccgg cgtcgcccaa
     6661 gccttcgccc cctctggtgc ccacccgctc accgtcggag ccatgcggct gtccgtcggc
     6721 gcgatcgccc tcatcatctt cgtcctggct cgtggtgaca ccttcggttc cgtcgtccgg
     6781 ctgtggggga gcccagccgg gctgatcgcg acggccgggg ccgcggcata ccaaccgttc
     6841 ttcttcgccg gggtggcgcg ggtcggcgtg ccgctgggca cgttggtcgc cgtggggagc
     6901 gctcccatct tcaccgggct actcggctgg gtctggcggc gcgagcgccc aacgggcacc
     6961 tgggcgctgg ccaccaccat ctgcgtggcc ggactggcgc tcctgaccgg cgcgggtgcc
     7021 caggtcggga gcctcgacgg gatcgccttg accgcaggag caggagtgtc gatcgcctgc
     7081 tacacggtcg cggcgaagtc gctcctggag tcgggtgtgg atgccatcgg agtgtgcgcc
     7141 tcgaccttcc tgcttggcag catcgtcgtc ctgccgttcg cgctcggcca aggcatcggg
     7201 tgggccctga ccggcagcgg tgtcctcgtc gcgctctatc tcgggctggc caccatggcg
     7261 gtcgcgaaca tcctctacgc ccgcggcctg ctgcacctgc ccccggccac cgccgcgacc
     7321 ctggggctcg ccgacccggt caccgccacc gcgctcggag tcctcgtgct cggtcagacg
     7381 gtctcgccct tgggatggct cggcttcctc ggagtcgtcg tggggctgtt gctgcaaggg
     7441 atctgggcgg cgcgcgccgg gcggcgcgac caactccccc tgggctgagc gcgccgaaac
     7501 cggcgcgacc gtggacgcga aggcccgata accttgggta tgccgcgcgg ggcctgcccg
     7561 cgccactcac cgcagggagc ccgcagtgtc tgccagcatc accgtgtccg tcgccggaaa
     7621 cgagcgatcg gtcgcggagg cgactaccgc cgccgacctg ttcgccgatg accgagcgat
     7681 cgtcgttgcc cgggtcaacg gtgagcttcg cgacctggcc catgtcctcg ccgacggcga
     7741 cgccgtcgag ccggtgcgca tcgacgaccc cgacgggctg caggtgctgc gccactcgtg
     7801 cgcccacgtg ctcgcgcagg ccgtccagca ggtcaacccc gacgccaaac tcggcatcgg
     7861 cccgccgatc cgggacgggt tctactacga cttcgacgtg accacgccgt tcacgcccga
     7921 agacctcaaa gagctcgaaa aggtcatgca gcggatcatc aacgagggcc agaccttcgt
     7981 gcgccgcgag gtgagcgacg aggacgccct ggtcgagctg gcaggcgagc cgttcaagtg
     8041 cgagctcatc gggctcaagg gcgggtctgc cgaggacgcg gcggaaggtg ccggcgtcga
     8101 ggtcggcggc gcccagctga cgatctacga caacgtgcgc cgcgatggca cccgggcctg
     8161 gggtgacctc tgccggggcc cgcacatccc gacgaccaag ttgctcggca acgccttcaa
     8221 ggtcacacgc tccagcgcgg cctactggcg cggcgaccag gccaaccagc agttgcagcg
     8281 ggtctacgga acggcctggc cgacgaaggc cgacctcacg gcatacctcg accggctggc
     8341 cgaggcggaa cggcgcgatc accgcaaact cgggatcgag cttgacctct actccttccc
     8401 ggacgagctc ggcagcgggc ttccggtgtt ccaccccaag ggcggggtcc tcaagcggga
     8461 aatggaggac tacgcgcgac ggcggcacat cgaggagggc ttcctctacg tcggcacgcc
     8521 gcacatctcc aaggaggggc tcttccacac ctccgggcac ctgccctact acgccgacac
     8581 gatgttccct cccatgagca tggaggggtc ggattactac ctcaaagcga tgaactgtcc
     8641 gatgcacaac ctcatctacc gctcgcgggg acgttcctac cgcgagttgc cgttgcggct
     8701 gttcgaattc gggtcggtct atcggtatga aaagtcgggg gtcatccatg gcctcacccg
     8761 ggtgcgtggt ctggagatgg acgactcgca ctcctacgtc acgcccgagc aggcccccgc
     8821 tgagatcaag cacctgttga acttcgtgct gtcgctgctg cgtgacttcg gcctggacga
     8881 cttctacctc gagctgtcca cgcgcgacga ctccggcgcc aagaagggca agttcatcgg
     8941 ctcggaagag cagtgggccg aggcgacgca gatcctcgct gacgtggcgg ccgagtctgg
     9001 cctcgacctc gtgcccgacc cgggcggcgc ggcgttctac gggccgaaga tctccgtcca
     9061 ggcccgcgac gccattggcc gcacctggca gatgtcgacg atccaatacg acttcaacca
     9121 gccggagcgc ttcgggttgg aataccaggc tgccgacggc tcccgccagc aaccggtgat
     9181 gatccactcc gcgaagttcg ggtcgatcga gcgcttcatg ggggtcttgg tcgagcacta
     9241 cgcaggcgcc ttcccgccct ggctgtcccc ggtccaggtc ctcggggtgc ccgtcgccga
     9301 ggagtatgcc ggttacctcc gggaggtcct aggcggcctc gcggcgcagg gtgtccgggt
     9361 cgagttggac gagagcgacg accgcttccc caagaagatc cgcaacgcct ccaagtcgaa
     9421 ggtgccgttc gtgctcatcg cgggggagga cgaccgagcc gccggcgcgg tgtctttccg
     9481 gttccgcgac
//
LOCUS       DAOMZY010000113         9392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_6925_length_9392_cov_4.972261, whole genome shotgun sequence.
ACCESSION   DAOMZY010000113 DAOMZY010000000
VERSION     DAOMZY010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9392
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6925_length_9392_cov_4.972261"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="PLK64_07885"
     CDS             complement(<1..860)
                     /locus_tag="PLK64_07885"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074223.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HPV79703.1"
                     /translation="MPQHPATPSTALPAFVGRHIGPGEADVAHMLAVVGQPSLDALVD
                     SALPHLIRSDAPLALAAADSEAAVIAELRALAGRNRVLRSMIGLGYYGTISPPVVTRK
                     ILENPAWYTAYTPYQPEISQGRLEALLNFQTVVSDLTGLPIAGASLLDEATAAAEAMT
                     LMRRSTRLPDGAVLVVDTHTFPQTLAVVQTRARPLGIGVVVADLSTVTTGEQLRAAAG
                     GVPVFGVLVQYPGADGQVRSLSALAAAAHEIGALLTAATDLLALTILAAPGEWGADIA
                     VGSAQRFGVP"
     regulatory      complement(935..1045)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(1162..1746)
                     /locus_tag="PLK64_07890"
     CDS             complement(1162..1746)
                     /locus_tag="PLK64_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPV79704.1"
                     /translation="MSAIAPPSRPAGVGPAQGLLFDDDLPELREDEGYRGPTACRAAG
                     ITYRQLDYWARTGLVEPSVRSATGSGTQRLYGFRDILVLKVVKRLLDTGVSLQQIRQA
                     ISHLRERGVEDLARITLMSDGASVYECTSADEVIDLLQGGQGVFGIAVGRVWREVEGS
                     LSALPSERAEDDEASAPVSAGDELSARRASRMMA"
     gene            complement(1912..2433)
                     /locus_tag="PLK64_07895"
     CDS             complement(1912..2433)
                     /locus_tag="PLK64_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HPV79705.1"
                     /translation="MIEVDVLGVRVEMPTNQPIVLLRERDGERYLPIWIGAAEATAIA
                     YAQQGVVPPRPLTHDLLKDTLEALGHALVRVRIDELRDKVFYATLVVDGTEISARPSD
                     GIALALRTASPILVHEDVFTAAGITMAPEEDDEVERFREFLDQVSPEDFEAPDGPGGL
                     GGSEGERPPSPEQ"
     gene            complement(2467..3177)
                     /locus_tag="PLK64_07900"
     CDS             complement(2467..3177)
                     /locus_tag="PLK64_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPV79706.1"
                     /translation="MAARRRGLSIGLVLERLQGEFPDLTISKLRFLETEGLVAPERAP
                     SGYRLYTEEDVRRLRYVLTAQRDRFWPLKVIRDALDAMDRGLTPATDTSGERPAVPTA
                     AADPDVPAAARLAAPPSLRLTEAELMGAAALDEAMFQALRGFHLLRPDATGHYDDAAL
                     GVARAAAALAAYGVEARHLRPFRTAAEREVGLVQQIVVPALASAGGRASGADPTAEVL
                     HHCLALHAALVRAELRRG"
     gene            complement(3188..3574)
                     /locus_tag="PLK64_07905"
     CDS             complement(3188..3574)
                     /locus_tag="PLK64_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPV79707.1"
                     /translation="MRFRGLGEIPEAFDPDGGLSAEDEATIGALRHGTALLVVVRGPN
                     NGARFLLDDAEVTSGRHPESDIFLDDVTVSRRHAVFVRDGNGYAVKDVGSLNGTYVNR
                     ASVDAAVLRNGDEVQIGKYRFLYYSR"
     gene            complement(3875..4264)
                     /gene="gcvH"
                     /locus_tag="PLK64_07910"
     CDS             complement(3875..4264)
                     /gene="gcvH"
                     /locus_tag="PLK64_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPV79708.1"
                     /translation="MSELSYPEDLRYTSDHEWVAVASDGTARVGITSFAQNALGDVVY
                     VSLPAVGATVSAGDACGEVESTKSVSDVYSPLDGVVTSVNVTLDTAPELVNSDPYGDG
                     WLFEMRPSDPEAVAHLLDAAAYQAVLG"
     gene            complement(4360..5172)
                     /locus_tag="PLK64_07915"
     CDS             complement(4360..5172)
                     /locus_tag="PLK64_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HPV79709.1"
                     /translation="MAVRRPRRRPGSKAGVVTREPEEPREVTWRRLRRMFGARATRAN
                     VLATVLTLALGFAIATQIQQNQEGGLATLRQDELVRVLDDVTQRSSRLDAQVRELEVQ
                     RNALQSGADRAKAATEQAQERLDSLAILAGTVKATGPGIRVTIADSSGAVRASALLDV
                     LQELRDAGAEVVQFGTVRIVAGSYFTDAGTSITADGQSLSRPYVILAIGDPQTLASAM
                     NIPGGIVDSMKRLGASASVDQLSTVTIDALHTPKTPRYAQPVPEPTVAPTGG"
     gene            complement(5175..5507)
                     /locus_tag="PLK64_07920"
     CDS             complement(5175..5507)
                     /locus_tag="PLK64_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HPV79710.1"
                     /translation="MIPAIGLFVGVVLGLLLHPSVPLWLQPYLPIAVIAALDAVFGGV
                     RAVLDGIFVDKVFVVSFLSNVLVAAFIVFLGDQLGVGSQLSTGVVVVLGIRIFSNVAA
                     IRRHLFKA"
     gene            complement(5504..6541)
                     /locus_tag="PLK64_07925"
     CDS             complement(5504..6541)
                     /locus_tag="PLK64_07925"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HPV79711.1"
                     /translation="MSPRRVDESMALITELREGSLEAGYAAASARRVAEGQPSHTSTR
                     SPLVIAFAVIVGFLLVVSAMALRSATSVVEQARAELIGQITARQEAGDALAEKVSALE
                     AEVAGARSAALGASGQSGVLSQLAFLQSVTGVVAVTGPGLTVTLDDSADAVSGADAAR
                     GSSGFEAGRVSAADLQIVSNGLWAAGAEAISINGQRLTTRSAIRFAGQAILVDFRPLV
                     RPYVVTAIGDAKALQSSFGSSPTGSYLKSLEDNYGIPVAISGSASLTCPAGVPSALVS
                     ATARPTGIPSTTAAPTSTATSTRSTSTTTSTTKTSTSKTSTAKTSTTKTATSPITRVT
                     TSSPSTTEIRP"
     gene            complement(6538..7143)
                     /locus_tag="PLK64_07930"
     CDS             complement(6538..7143)
                     /locus_tag="PLK64_07930"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPV79712.1"
                     /translation="MEERRDIAGDETPDTDRVLTVPNALSVLRLVGVPLFLWLLLRGD
                     DGWALVTLALSGITDYLDGKIARHFHLVSRVGQLLDPIADRLYIISTLLGMAWRDIIP
                     WWIVGVLLLREAFMAVLMLALKRVGQIGLPVHFVGKGATLNLLYAFPVILLGQVPGTV
                     GEWALPIGWAFAWWGIVLYWVAAALYAAQAAPILRARGRVA"
     gene            complement(7224..7634)
                     /gene="arr"
                     /locus_tag="PLK64_07935"
     CDS             complement(7224..7634)
                     /gene="arr"
                     /locus_tag="PLK64_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014922336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase"
                     /protein_id="HPV79713.1"
                     /translation="MTGVLDPGPFFHGTKADLQVGDLLTAGFRSNYRPEVLMNHIYFT
                     ALTDGAGLAAELAAGDAAPRVYIVEPTGPFENDPNVTDKKFPGNPTRSYRSVEPLRIV
                     GELSDWVRLTPEALQEWRDRLAAILADERGVIIN"
     gene            complement(7814..8803)
                     /locus_tag="PLK64_07940"
     CDS             complement(7814..8803)
                     /locus_tag="PLK64_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPV79714.1"
                     /translation="MTTMRAVVIEAPGPPEVLSVRSVPLPVPQDGEVLIRVMAFGLNR
                     SELHFRQGVAESGSFPRIPGIEAVGVVESAPGGTFAVGAQVATLMGGMGREFDGGYAE
                     FVKVPAGQVIPFTSHLSWATLGAVPEMLQTAAGSLDVGLQAEAGQTVLIRGGTSSVGM
                     AAAVLAKLRGLTVISTTRNPAKIGSLRSLGVDHVLIDDGQIAAAVRDLMPGGVDCAVE
                     LVGVNVLKDTLLATKPGGTVCFTGMLSDEWTIRDFYPMDWLPNGVRLTAYSGGAADLT
                     PQTLQGFLDAVAAGSAVVPVGRIYRLDEIVQAHHDMQNNVVGGKGVVLTDKAG"
     gene            complement(8954..9313)
                     /locus_tag="PLK64_07945"
     CDS             complement(8954..9313)
                     /locus_tag="PLK64_07945"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HPV79715.1"
                     /translation="MRFLVDAQLPARLAEALTAAGHDAVHTSQLPDGNRATDHQVAEA
                     ADSSDRVVVTKDSDFRDGHLLAGRPRRLLVVGTGNITNNALLALFDASLVDIVEAFEE
                     ADFIELQTDSLIVHPRR"
BASE COUNT         1525 a         3158 c         3294 g         1415 t
ORIGIN      
        1 atgggcacgc cgaagcgctg ggccgagccg acggcgatgt ctgctcccca ctcccccggc
       61 gcggcaagga ttgtcagggc cagcaggtcg gtcgcagcgg tgagcagcgc gccgatctcg
      121 tgcgcggcgg cagcgagagc actcaacgac cgcacctgac cgtcggcccc ggggtactgc
      181 acgagcacgc cgaaaaccgg caccccgccg gcagcggcgc gcaactgttc accggtcgtc
      241 accgtggaga ggtcagcgac gacgacgccg atgcccagtg gccgggcccg ggtctgcacg
      301 acggcgagcg tctgggggaa ggtgtgcgtg tcgacgacca gcaccgcgcc gtcgggcagc
      361 ctggtggagc ggcgcatgag ggtcatcgcc tcggcggccg ccgtggcctc gtccagcagc
      421 gacgcaccgg cgatcggcag cccggtcagg tccgacacca ccgtctggaa gttgagcagc
      481 gcctcgagcc ggccttggga gatctccggc tgatacgggg tgtaggccgt gtaccacgcc
      541 gggttctcga ggatcttgcg cgtgacgacc ggcgggctga tggtgccgta gtagcccagc
      601 ccgatcatcg atcgcagcac gcggttgcgc ccggccagcg cacgcaactc ggcgatgacc
      661 gcggcctcgg agtcagcggc ggccagcgcc agcggcgcgt cgctcctgat gaggtgcggc
      721 agggcggagt cgacgagggc gtcgagcgag ggctgcccga cgaccgccaa catgtgcgcg
      781 acgtcggcct cccccggacc gatgtgcctc ccgacgaagg caggcagggc ggtcgagggg
      841 gtggcagggt gctgcggcat acgagcctcc gggcaacgtc gaggacgacc aacaaggccc
      901 cctctgtcga gccgccgtgg cccctccaga gtcgcctgtc cacgtggtcc ggttgcctga
      961 gaggttccgg ggtagttgcc ccttcggcgc caccttggcc aaggcccgca ggcccggcgt
     1021 ggatggactc tcccacgtgg agtgatcagc ggcactgagt gtagccgccg aacgggcggg
     1081 caccgcgtca gtcgcggccc gtccacgcag gggtatgccg cccgcgcgac ctctcgcgcg
     1141 gcataccggc tcgcggtggg gtcaggccat catccgggag gcgcgacggg ccgacagctc
     1201 atcgccggcc gacacgggcg cgctcgcctc gtcgtcttcg gcacgctcgc tcggcagcgc
     1261 ggacaacgac ccctcgacct cgcgccacac ccgacccacg gcgatcccga acactccctg
     1321 cccaccttga aggaggtcga tgacctcgtc ggcggaggtg cactcataga cggaggcgcc
     1381 gtcgctcatg agcgtgatgc gggccaggtc ttcgacaccg cgctcgcgaa ggtggctgat
     1441 ggcctgtcgg atctgctgaa gggacacgcc ggtgtcgagc agtcgcttga cgaccttgag
     1501 cacgaggatg tcacggaaac cgtagaggcg ctgggtccct gagccggtgg cgctgcgcac
     1561 ggacggctcc acgaggccag tgcgggccca gtagtccaac tgccggtagg tgatcccggc
     1621 ggcgcggcat gcggtcgggc cgcggtagcc ctcgtcctcg cgcagctccg gcagatcgtc
     1681 gtcgaagagc agcccttgcg ccggaccaac gccagcgggg cggctgggcg gggcaatcgc
     1741 gctcacgggt gtcctcctgg tggtccgaac gccaggcgcg tagttacacc cgtgctcttc
     1801 ttcccttgac ggtagggccc gagggcctca gggtcaatca tcactgcacc ttttgtcacg
     1861 cgtgtcgcag atatcacttg cgtaacataa ctctcaggta caggttgaca gttactgctc
     1921 cggggacggt gggcgctccc cttcggaccc acccagccct cccgggccgt ccggagcctc
     1981 gaagtcttcc ggggacacct ggtcgaggaa ctcgcggaag cgctcgacct cgtcgtcctc
     2041 ttcgggagcc atcgtgatgc ccgcggccgt aaagacgtcc tcgtggacga ggatcgggct
     2101 ggccgtacgc agggcgagag cgatcccatc tgagggccga gccgagatct cggtgccgtc
     2161 aaccacgagc gtcgcgtaga acaccttgtc gcgcagttca tcgatgcgca cccgcacgag
     2221 ggcgtgcccc agcgcctcca gggtgtcctt gagcaggtcg tgcgtcagtg gacgcggcgg
     2281 cacgacgccc tgctgggcat aggcgatggc tgttgcctcg gcggcgccga tccagatggg
     2341 cagatagcgc tccccgtcgc gttcgcgcaa caggacgatg ggctggttgg tgggcatctc
     2401 cacgcgaacg cccaagacgt cgacctcgat cacccggccc acgctacccc ggtggcccgc
     2461 ctcgggctat ccgcgccgca actcggcccg caccaaggcc gcgtgcaggg ccagacagtg
     2521 gtgcaggacc tcggcggtcg ggtccgcgcc cgaggcccgg ccgccggctg aggcgagggc
     2581 cgggacgacg atctgctgga ccagcccgac ctcccgctcg gccgccgtcc ggaacggccg
     2641 caggtggcgg gcctcgacgc cgtatgccgc cagcgcggcg gcggcgcggg ccaccccgag
     2701 ggcggcgtcg tcatagtggc ccgtggcatc agggcgcagc aggtggaagc cccgcaaggc
     2761 ttggaacatc gcctcgtcga gcgcggcggc ccccatcaac tcggcctcgg tgaggcgcaa
     2821 actcgggggt gcggccagcc tggcggcagc agggacgtcg gggtcggcgg ccgcggtcgg
     2881 cacggcgggc cgctcccccg acgtgtccgt agccggggtg agaccgcggt ccatggcgtc
     2941 gagggcgtcg cggatgacct tcagcggcca gaaccggtcc cgctgggcgg tgaggacgta
     3001 gcgcagtcgg cggacgtcct cctcggtgta gaggcgatag cccgacggcg cgcgctcggg
     3061 cgcgacgaga ccttcggtct cgagaaagcg aagcttcgag atcgtcaggt cggggaactc
     3121 accctgaagt cgttcgagga ccaggccgat cgagagccca cggcgacggg cagccacagc
     3181 ggccccgtca gcgggagtag tagaggaagc ggtacttgcc gatctgcact tcgtcaccgt
     3241 tgcgcaggac ggccgcgtcc actgaggcgc ggttgacata ggtgccgtta agcgaaccaa
     3301 cgtccttcac ggcataccca ttgccatccc ggacgaagac cgcgtgacgc cgcgagacgg
     3361 tgacgtcgtc gaggaagatg tcgctctcgg ggtgccgccc cgaggtcacc tcggcgtcgt
     3421 cgagaaggaa acgagccccg ttgttgggcc cccggacaac gacgagcaag gccgttccgt
     3481 gtcgcaacgc accgatggtc gcctcatcct cggcagagag ccccccgtcg gggtcgaagg
     3541 cctcggggat ctcacccagg ccgcggaacc gcatcgtcgt ggggccgccg ggacgcgacg
     3601 gaagcgagcg tcgagtcagg tcttcctgac cctccggggc gcccgtctcg tcgttctcgg
     3661 ggcgctcgtg ctcgtccgac attgcttctc cgtttccgca cacgggcttg cctggtgtct
     3721 atgcccgttt gacgtcaccg aggtccgcgg gccattcggt gatcgttcgg gaagaatacg
     3781 tcatggcgcc gacccccggc caggcgtccc gtcccgggca ttcccgggat cgaatcatac
     3841 gcccgcgacg tttctgtcgc ggcagatacg gggactatcc cagcactgcc tggtatgccg
     3901 cggcgtccag caggtgtgcg accgcctccg ggtcgctcgg acgcatttcg aacagccagc
     3961 cgtcgccata agggtcgctg ttgaccaatt cgggcgcggt gtcgagcgtg acgttgaccg
     4021 acgtgacgac tccgtcaagg ggggaataga catcgctgac cgatttggtc gattcgacct
     4081 caccgcaggc atcgccggcg ctcaccgtgg cacccaccgc cggcagggag acgtaaacca
     4141 cgtcgccgag ggcgttctgg gcgaaggagg tgatgcccac ccgagccgtg ccgtcggacg
     4201 ccacggcgac ccactcgtga tcgctggtgt agcgcaggtc ctcggggtag gacaactcgc
     4261 tcatggctct ccttcgggtg gcggcgtggt tcggacgttc tcggccggga tatcggggtg
     4321 tcgggatgtc agggtgtcgc aggcgctgcg gagccgacct tagcccccgg tcggggcgac
     4381 cgtgggttcg ggcaccggct gagcgtaacg aggggtcttc ggcgtgtgca acgcgtcgat
     4441 ggtgacggtg ctcagttggt cgaccgaggc gctggcgccg aggcgtttca tcgagtccac
     4501 gatgccgccg gggatgttca tcgccgaggc gagagtctgg gggtcgccga tggcgaggat
     4561 gacgtaggga cgcgacaacg actgcccgtc ggccgtgatc gaggttcccg cgtcggtgaa
     4621 gtaggacccg gcgacgatcc gcaccgtgcc gaactgaacg acctcggcac ccgcgtcgcg
     4681 cagctcttga aggacgtcga gcagcgccga ggcacgcacg gctccggagg agtcggcgat
     4741 ggtcacccgg atgccgggcc ccgtcgcctt gacggtgccc gcgaggatgg cgaggctgtc
     4801 cagccgctcc tgcgcctgct cggtggccgc cttggcccgg tcggcgcccg attgcagggc
     4861 gttgcgctgc acctccaact cgcggacctg ggcgtcgagt cgcgacgagc gttgcgtgac
     4921 gtcgtcgagc acgcgcacca actcgtcctg gcgcagggtg gccaggccac cttcctggtt
     4981 ctgttggatc tgggtggcga tggcgaaacc cagcgccaga gtgaggacgg tggccaagac
     5041 gttggctctg gtcgcgcggg caccgaacat ccggcgcaac cggcgccagg tgacctcgcg
     5101 cggctcctcc ggctcgcgag tgaccactcc ggccttcgat ccggggcgac gacggggtcg
     5161 gcggacggcc atcgtcaggc cttgaacagg tggcggcgga tggctgccac gttcgagaag
     5221 atccggatgc caaggacgac gacgacgccc gtggacagtt gcgagccgac gcccaactgg
     5281 tcgccgagga agacgatgaa ggcggcgaca agcacgttgg acaggaacga cacgacgaag
     5341 accttgtcga cgaagatccc gtcgaggacg gcgcggacac cgccgaacac cgcgtcgagc
     5401 gcggcgatga cggcgatcgg gagatacggc tgcagccaca gcggcacgct ggggtgcagc
     5461 aacaggccga ggacgacccc tacgaacagg ccgatggcag gaatcatggc cgtatctccg
     5521 tcgtgctcgg tgaggacgtg gtcacccggg tgatcgggga cgtggctgtc ttggttgtcg
     5581 acgtcttggc tgtggatgtc ttggacgtgg acgtcttggt tgtggacgtg gtcgtcgacg
     5641 tggatcgcgt ggacgtggca gtcgacgtgg gggccgccgt ggttgagggt atgccggtcg
     5701 gccgcgccgt cgcggacacc agcgcgctgg gcacgcccgc ggggcaggtc aaggaagcgc
     5761 tcccggagat agccacgggg atgccgtagt tgtcctcgag cgacttcagg taactaccgg
     5821 tcggcgagga cccgaacgaa ctctgcaggg ccttggcatc gccgatcgcc gtcacgacgt
     5881 agggccgcac caggggccgg aagtcgacaa ggatggcctg tccggcgaac cggatcgctg
     5941 agcgcgtggt caggcgttgc ccgttgatcg agatggcttc cgcgccagcc gcccacaacc
     6001 cgttgctgac gatctgcagg tcggcggcgc tcacccgccc cgcttcgaac ccgctggacc
     6061 cgcgcgcggc atccgcgccg ctgaccgcat cggccgagtc gtcgagggtc acggtgagcc
     6121 cgggacccgt gaccgcgacg actcccgtca cggactgcag gaaggccagt tggctcaaga
     6181 cgccggactg ccccgatgca ccgagggccg ccgatcgcgc ccctgccact tctgcctcga
     6241 gtgcgctcac cttctcggcc agcgcgtcgc cagcctcttg gcgggccgtg atctggccga
     6301 tgagctcggc gcgggcctgc tcgacgacgc tcgtcgccga gcgcagcgcc atggcggaca
     6361 cgacgaggag gaacccgacg atgaccgcga aggcgataac caacggactg cgggtggagg
     6421 tgtggctcgg ttggccctcg gcgacccggc gagccgacgc cgcggcatac cccgcctcga
     6481 gtgacccctc gcgaagctcg gtgatgaggg ccatcgactc gtcgacccgg cggggactca
     6541 tgcgaccctc ccccgggcgc gcagaatggg agcggcttgg gcggcataca gggcggcggc
     6601 gacccagtag aggacgatcc cccaccacgc gaacgcccaa ccgatgggca gagcccactc
     6661 cccgaccgtc ccaggcacct gcccgaggag gatgacgggg aaggcgtaga gcaggttgag
     6721 cgtggcgccc ttgccgacga agtgcaccgg caggccgatc tgcccgaccc gtttgagggc
     6781 gagcatgagc acggccatga aggcctcgcg cagcagcagg accccgacga tccaccaggg
     6841 gatgatgtcg cgccaggcca tgcccagcag cgtgctgatg atgtagaggc gatcggcgat
     6901 cgggtcgagc aactgcccca cgcgggagac caggtggaag tgccgagcga tcttgccatc
     6961 gagatagtcg gtgattccgg agagggcgag cgtgaccagg gcccagccgt cgtcgccgcg
     7021 cagcagcagc cagaggaaga gcggcacacc caccagtcgc agcaccgaca gggcgttggg
     7081 cacggtgagg acgcggtcgg tgtcgggcgt ctcgtcgccc gcgatgtccc tgcgctcctc
     7141 cacggccccg agcctaaccc gcgcgggtga cgatccccgc ttggtcgcct ccgccccgcc
     7201 ggtatgccgc ccgcgaccgc tcctcagttg atgatgacgc cgcgttcgtc ggcgaggatg
     7261 gcggccaacc ggtcccgcca ctcctggagg gcctcgggcg tgaggcgcac ccagtcgctc
     7321 agctcaccga cgatcctcag gggctcgacg ctgcggtatg agcgggtcgg attgccgggg
     7381 aacttcttgt cggtcacgtt ggggtcgttc tcgaagggtc cggtcggctc gacgatgtag
     7441 acgcgagggg cggcgtcgcc cgcagccaac tccgcggcga gcccagcgcc gtcggtcagc
     7501 gcggtgaagt agatgtggtt catcagcacc tctggccgat agttcgaccg gaagccggcg
     7561 gtgaggaggt cgcccacctg caggtcggcc ttcgtcccat ggaagaacgg ccccggatcc
     7621 agcacgccag tcacctgcgg cagtctacga gggcgtcctt gaagcggttc tggacgtcga
     7681 tgccggatgt gtgagtgatg tctgggttgc gcgtgcggca cggagtgcga tcagcggggt
     7741 gatcacccca tcaatcgcac tccctcgcgc actcgcatga ccagtcgaat caatccagcc
     7801 ggcccacccc tggctacccg gctttgtcgg tgaggacgac acccttgccg ccgacgacgt
     7861 tgttctgcat gtcgtgatgg gcctggacga tctcgtcaag gcggtagatc cggccaacgg
     7921 gcaccaccgc cgaccccgcg gcgaccgcgt cgagaaaccc ttgcagcgtc tgcggcgtca
     7981 gatcggccgc cccacccgaa taggccgtga gccgaacgcc gttcggcagc caatccatcg
     8041 gatagaagtc acgaatcgtc cattcgtccg acaacatgcc ggtgaagcag acggtgcctc
     8101 ctggcttggt cgcgagaagg gtgtccttca ggacgttgac acccaccagt tcgacggcac
     8161 agtcgacacc cccaggcatc aggtcgcgca ccgcagcagc gatctggccg tcgtcaatga
     8221 gcacgtggtc cacaccgagg ctgcggagcg agccgatctt ggccgggttc cgagtcgtgg
     8281 agatgacggt cagcccgcgc aacttggcca gcaccgcagc agccatcccg accgaggagg
     8341 tgccgccgcg gatgagcacc gtctgccccg cctcggcctg gaggccgaca tcgagggaac
     8401 cggctgcggt ctgcagcatc tcgggcacag cacccagcgt tgcccacgaa aggtggctgg
     8461 tgaacgggat cacttgcccc gcaggaacct tcacgaactc ggcatatccc ccgtcgaact
     8521 ctcgacccat cccgcccatc aacgtcgcca cctgagcgcc gacggcgaac gtgcctcccg
     8581 gcgcggactc gacgacgccg acggcttcga tgcccggaat cctcgggaag ctccccgact
     8641 cggccacgcc ctgcctgaaa tgaagctccg accggttcag gccgaaggcc atcaccctga
     8701 tcagcacctc gccgtcttga ggcaccggaa gtgggacgga gcgcacgctc aagacttctg
     8761 ggggtccagg ggcctcgatc accacagcgc gcatcgtggt catgctcgga tccccttgcg
     8821 gcccatgaga actcacctac cgagattgtg atggagatgt gcagttgccc tagcagacta
     8881 tgcggaatcc gccctcggac ctgcaggaac atcggtcgat cggtctctca agtcacggct
     8941 tctgactcac ctgctacctg cgtggatgca cgatcagaga gtcggtctgc agttcgatga
     9001 agtcagcctc ttcgaaggct tcgacgatgt cgacaagact tgcgtcgaac agcgctagga
     9061 gcgcgttgtt ggtgatgttg ccggtgccga cgacgagcag gcgccggggc ctccctgcga
     9121 gcaggtggcc gtcgcggaag tcgctgtcct tggtgaccac cacacgatcc gaggaatccg
     9181 ccgcctcggc cacctgatgg tccgtggcgc ggttcccgtc aggcaactga ctcgtgtgta
     9241 cggcgtcatg gcccgctgcc gttagcgcct ccgcaaggcg ggcaggcagc tgggcgtcga
     9301 cgaggaatct cacgccgcgc cgcgtgggaa cacgcggtga ccgcctgatg ccagggcacc
     9361 gaactcgagg gcggctagca agtcctcacg ct
//
LOCUS       DAOMZY010000114         9362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_6952_length_9362_cov_6.266359, whole genome shotgun sequence.
ACCESSION   DAOMZY010000114 DAOMZY010000000
VERSION     DAOMZY010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9362
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6952_length_9362_cov_6.266359"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="PLK64_07950"
     CDS             complement(<1..161)
                     /locus_tag="PLK64_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HPV79716.1"
                     /translation="MPNRLAQALSPYLRQHADNPVDWWEWSEAAFAEARHRDVPVLLS
                     VGYAACHWC"
     gene            285..1535
                     /locus_tag="PLK64_07955"
     CDS             285..1535
                     /locus_tag="PLK64_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HPV79717.1"
                     /translation="MANYVARIADHELDARLRVMGAVLIEGPKACGKTATASQIANTI
                     IRLDEDPVARAQLDFDPQALFSGRPPILFDEWQVEPEIWNRVRRQVDDREEKGQFILT
                     GSARPRDNANRHSGAGRFSVMQMRPMSLFETGHSTGEISLANLLAGERQAGLGTHLSF
                     DNLIRRIITGGWPDLLDSDEDFARDWLADYLRQVIEVDVPEMGHRRNPGNLRRLLESL
                     ARGVGQPVKLSDLAKDVGGEGGPVAHETITGYLDALRRLWLTDDSAAWRPHMRSRTRL
                     RTAPVRYFVDPSIGTAALSIGSAELKRDPLALGFHFEALAIRDLRIYAQLHRGQVDSW
                     RDANGNEVDAVLTARDNTWAAFEIKLNPRDVDAGAASVLKFAANVDTDRHKRPACLGV
                     ITSTGAGGLRPDGVHVIPIGALGP"
     gene            1624..1890
                     /locus_tag="PLK64_07960"
     CDS             1624..1890
                     /locus_tag="PLK64_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79718.1"
                     /translation="MKIFAVVLVTATAFGASWVLTRWASSPVWHRVVVLVALPAALVL
                     ASFNSDPLSGTTAVIAVLVGAAVGATFRPRVSGREGGRSTGPGQ"
     gene            complement(1847..2674)
                     /locus_tag="PLK64_07965"
     CDS             complement(1847..2674)
                     /locus_tag="PLK64_07965"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPV79719.1"
                     /translation="MSGAMGGAAGGVGEATGTVGDSGADVRVHWVTAFLDRPVAAFGL
                     AADFWCAALGATLSARRGENGEFATLLPTDGDAAIKLQGVGDPCQGGHLDLEVPDVVA
                     ARDRAVGLGARSAADLGSVQVLRSPAGLLFCFVPLRSGGVRPPVVETPGGRVRADQVA
                     LDVGPASAPAEVEFWAALTGWRVVASVRPEFTVLIPPPELAIRLLLQRLDTEPSNGPR
                     MHLDLACADVSAATAYHRSLGAQVVAVQEFWTVLRDPAGEVYCLTARDPSTGRLPGH"
     gene            complement(2671..3618)
                     /locus_tag="PLK64_07970"
     CDS             complement(2671..3618)
                     /locus_tag="PLK64_07970"
                     /inference="COORDINATES: protein motif:HMM:NF016533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HPV79720.1"
                     /translation="MALPAGLVAAAAGRPADVLLDGPSARHAIDGDSWLRGLPRTVAD
                     VCADWELTPDGPARHGACAIALPVVSADGPAMLKVTWPHHEAAGEHLALRHWGGQAAV
                     RLLRADPSLWALLLERLDADRDLHGIPMDEACEVVGDLLRHLKVPAPPTVPTLREYAA
                     RKADAYADVLAGGAGPVPRRFVDQARALAADFATDDSGGGWLLHTDLHCSNVLRGIRD
                     GRPAWVAIDPKPAAGDRAFDVVALLWDRPDELGTGADLRWGLRRRVEIVCEAAEVDED
                     RARSWAIVREMDNALEVAHDPAAADRVSLAVAIIKAMQP"
     gene            complement(3624..4664)
                     /locus_tag="PLK64_07975"
     CDS             complement(3624..4664)
                     /locus_tag="PLK64_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79721.1"
                     /translation="MSRAPLTAGLGGIPRRMPPRRTVLRAAGLAGLSAVAGIGLAGCG
                     IRLEDDAPTIPFVSRQPIPDEALLVAAYRRATALAGVAGRATGVPLAPEVTQRHTRQA
                     QVLRAILETGQVPERIISGPPGSTATATSGPTAPVDGTATTASTSAPTNAVPPPVTAG
                     ELARAARDTADASIAAAGSFVTQRALGVAIAAHDAAGAASLAAPPVWPTRDPLPAPVA
                     TPLVGVTRACEYALEVAAAHLLDTDRESLLATVAALTRREATLAAALTPDPLPALGYH
                     LPFPVTGPDDARRLVSVVLRNLVDRGLDDLDRIPQGSSALLEVVRLETEAVTLAVAHG
                     VAWPTMPGLALG"
     gene            4807..>5378
                     /locus_tag="PLK64_07980"
     CDS             4807..>5378
                     /locus_tag="PLK64_07980"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HPV79722.1"
                     /translation="MQASLVRSIVEAPLAAVDLVVEDVTVTAAGRRSVVRIAVDRSLA
                     GLANTDTTSFVAPLSLDEVADATRVVDAALDSDTRVGNAPYVLEVSSPGVDRPLTQPR
                     HLRRNVGRLVRLRLKEGAERSGRIASVSDLVIVLTPDPAADPAATAAGPVEIPVDQVR
                     DGRVIIEFGRIDEVDLDEPDDELDETDETD"
     assembly_gap    5379..5478
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5535..6698
                     /gene="nusA"
                     /locus_tag="PLK64_07985"
     CDS             5535..6698
                     /gene="nusA"
                     /locus_tag="PLK64_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPV79723.1"
                     /translation="MDIDLAALRALERERDLSLEVLIPAIEQALLTAYHRTDGSFKIA
                     RVELDRKTGHVVVLAREEGPLLPPTEEGERPTRGEPGPEFDDTPDGFGRVAAATARQV
                     IVQRLRDLEDEAILGDFKGREGDIVSGIVQQGSDPRNVLVDFGTVEGMLPQAEQVPGE
                     VYRHGERLRCYVVSVKRGPKGPQIALSRTHPNLVRSLFKLEVPEIADGTVEIAAMIRE
                     AGHRTKIAVRSRVPGVNAKGACIGPMGARVRAVMNELGGEKIDIVDYSDDPREFIAAA
                     LSPARVQSVEVVDPVLRAARAIVPDYQLSLAIGKEGQNARLAAKLTGWRIDIRSDTAV
                     AENPPTSDAPPASATRPAGDRRLQQGGGGRHTGSRGTGRGGSGAGPSGPPRRG"
     gene            6730..7128
                     /locus_tag="PLK64_07990"
     CDS             6730..7128
                     /locus_tag="PLK64_07990"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HPV79724.1"
                     /translation="MGTDRTGLRSASFPYAAQQGSPVRTCVGCRRPDSWLVLVRVVAI
                     VEAGQARLVPDVRHRLPGRGAWLHPETACLEVAVRKRAFGRALRLQVPADAAALTAYL
                     RDLVPPLEPTMSVQPQGGLDADEHAMSTQQ"
     gene            7192..>9362
                     /gene="infB"
                     /locus_tag="PLK64_07995"
     CDS             7192..>9362
                     /gene="infB"
                     /locus_tag="PLK64_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014921271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPV79725.1"
                     /translation="MAKVRVSALAKELGTDSKSLMAHLSALGEYVKSPSSTIESPVER
                     KVRETFPKPAAASAPAPAAAAPVARAATPAAPAAPAAPAPVTPSAPAPAAAAPVAPAA
                     AAPAPAPARSGVPAPSAPAPAAPAPSAPAAPAPAAPAVQAPAAQAPVAAPAAPVAPRP
                     AAAGPSAPAAPAAPTSAPSGAPSGPVPGPRPAPRPGSPRPGNNPFAPSQGMGRPPREG
                     GPARPGNNPFAPNQGMPRPQARPGAAGPGGPRPAAAGPGGPRPGGPRPNPGMMPDRAS
                     VARPGTRPAPGARPGGPGRPGGGGGGFGGRPGGGGAPGGAGAGGGGGFGGRPGAPGRG
                     GTQGAFGRGGGRPVRGRKSKRAKRQEFEQMQAPTIGGVSVPRGDGTTVVRVRRGSSLT
                     DFADKVNANPASLVTVLFHLGEMATATQSLDEDTFKLLGAELGFVVEVVSPEDEEKEL
                     FAAFNIDLDAVEDDEELLVARPPVVTVMGHVDHGKTKLLDAIRHANVIATEAGGITQH
                     IGAYQIVTQHEGIDRAITFIDTPGHEAFTAMRARGAKVTDIAILVVAADDGVMPQTIE
                     ALNHAQAADVPIVVAVNKIDKEGANPDKIRQQLTEYNLVAEEYGGDTMFVDVSAREKL
                     NIEGLLEAVLLTADAALDLRANPDTDARGVAIEANLDRGRGATATVLVQQGTLRVGDA
                     IVTGSAHGRVRALLDEHGNNLDVATPSRPVQVLGLSSVPRAG"
BASE COUNT         1442 a         3362 c         3176 g         1282 t
ORIGIN      
        1 tggcaccagt ggcacgcggc atacccgacg gacaacagga ccgggacgtc gcggtgacga
       61 gcctccgcga aggccgcctc cgaccactcc caccagtcga ccgggttgtc ggcatgttga
      121 cgcaggtagg ggctgagggc ctgcgcgagt cggtttggca tgcctgtcaa ggtaaccggt
      181 tcgggcgggt gcagttgctt gaggtcgagt gcgagctagt caaaacgtca gatccagcaa
      241 ccgcgaaagc tcgacttcaa gttaggcaaa cgatagagtt tggcatggct aactacgtcg
      301 cgcgcatcgc cgaccacgag cttgacgccc gactacgcgt gatgggcgca gtcctcatcg
      361 aggggccgaa ggcgtgcgga aagaccgcca ctgcatccca gatcgccaac acgatcatcc
      421 gcctcgacga ggaccccgta gcgcgcgccc agctcgattt cgacccccaa gccctcttct
      481 ccggaaggcc accgatcctg ttcgacgaat ggcaggtcga gccggagatc tggaaccgcg
      541 tccgtcggca ggtcgacgac cgcgaggaaa agggccagtt catcctcacc ggatccgccc
      601 gaccgcgaga caacgcgaac cgacactcgg gggccgggcg attctcggtc atgcagatgc
      661 ggccgatgag tctcttcgag accgggcact cgaccggcga gatctccctg gcgaacctcc
      721 tggcggggga acgccaggcc ggcctgggca cgcacttgag cttcgacaac ctcatccgtc
      781 gcatcatcac gggcggctgg ccggacctac tcgactcaga cgaggacttc gcacgagact
      841 ggctcgccga ctacctgcgt caagtcatcg aggtcgacgt cccagagatg ggccatcgcc
      901 gtaacccagg caacctgcgc cgccttctcg aatccctagc tcgtggcgtc ggacagcccg
      961 tcaagctcag cgatctcgcc aaggacgtcg ggggcgaggg cggcccggtc gcccacgaga
     1021 cgatcaccgg ctacctcgac gcactcaggc gtttgtggct caccgacgac tctgcagcgt
     1081 ggcgacccca tatgcgatct cgaacccgcc tgcgcaccgc gccggtgcgc tacttcgtcg
     1141 acccatcgat cggcacagcc gcactcagca tcggcagcgc cgagctcaag cgggaccctc
     1201 tcgcgctggg cttccatttc gaagcgctgg ccatccgcga cctccgaatc tacgcacagt
     1261 tgcaccgtgg gcaggtcgac tcatggcggg acgcaaacgg caacgaagtc gatgcggtcc
     1321 tgaccgcccg cgacaacacg tgggcagcct tcgaaattaa gctcaaccca cgcgatgtcg
     1381 acgccggggc agcatccgtc ctcaaattcg ccgcgaacgt cgacaccgac cgacacaagc
     1441 ggccggcctg cctgggtgtc atcacctcga cgggcgccgg tggcctccgt ccagacggtg
     1501 tccacgtcat cccgatcggc gcgctcggcc cctgaactcg ctgccgaacg tcaaacgatt
     1561 ggcgagatgc ttaacccgtt cgccgaacgc gccacatcaa gcgaccactg gaggtagcaa
     1621 cccatgaaga tctttgccgt tgtgctggtg accgcaacag ccttcggtgc ctcgtgggtg
     1681 ctgacgcgct gggcgtccag ccccgtctgg caccgcgttg ttgtgctcgt cgcccttccc
     1741 gcggccctcg ttctggccag cttcaacagc gaccctctgt cgggcacgac cgccgtcatc
     1801 gcggtcctcg tgggcgccgc ggtcggcgcg accttccgac ctcgggtcag tggccgggaa
     1861 ggcggccggt cgacgggtcc cgggcagtga ggcagtagac ctcacccgcg gggtcacgca
     1921 ggacggtcca gaactcctgc accgccacca cctgcgcccc aagcgagcga tggtatgccg
     1981 tcgcggcgct gacgtcggcg caggccaggt ccaggtgcat ccggggtccg ttgctcggtt
     2041 cggtgtccag gcgctgcagg agcaggcgga tcgccaactc gggcggtggg atgagcacgg
     2101 tgaactcggg gcgaaccgag gcaaccactc gccacccggt gagcgccgcc caaaactcca
     2161 cttcggcggg cgcgctcgcc gggcccacgt cgagggcgac ctggtcagcg cgcacccgtc
     2221 caccgggcgt ctcgacgacc ggtggacgga ccccgccgct gcgcagcgga acgaagcaga
     2281 agagcagccc ggcaggggag cgcaacacct ggaccgaccc gagatccgca gctgaccggg
     2341 ctccgaggcc gacggcgcgg tcgcgagcgg cgacgacgtc ggggacctcc agatcgaggt
     2401 gtccgccctg gcacgggtcg ccgacccctt ggagtttgat cgcagcgtcg ccgtcggtcg
     2461 gcagcagggt ggcgaactcg ccgttctcgc cccgacgcgc cgacagggtc gccccgaggg
     2521 ccgcacacca gaagtcggcg gctaagccga aggccgcaac cgggcggtcc aggaacgccg
     2581 tcacccagtg cacccgaaca tcggcgccgc tgtcaccgac cgtgcccgtc gcctcgccga
     2641 caccgcccgc ggccccgccc atcgccccgc tcatggctgc atcgccttga tgatcgccac
     2701 cgcaaggctg acgcggtccg cagccgcggg gtcgtgagcc acctcaagtg cgttgtccat
     2761 ctcgcggacg atagcccagg accgagcccg gtcctcgtcg acttccgcgg cctcgcacac
     2821 gatctccacc cgtcgacgca gcccccaccg aaggtcggcc ccggtgccca actcgtcggg
     2881 ccggtcccac aacagcgcga ccacgtcgaa ggcgcggtcg ccggccgccg gcttggggtc
     2941 gatcgccacc catgccggcc ggccgtcgcg gatcccgcgc aggacgttgg agcagtgcag
     3001 atcggtgtgc agcagccatc caccgcctga gtcatcagtg gcgaaatctg cggccaacgc
     3061 cctcgcctgg tcgacgaacc gccgaggcac cgggccggcg ccaccggcga gcacatccgc
     3121 gtacgcatcc gccttccgcg cggcatactc acgcagcgtt ggcacggtcg ggggagcggg
     3181 caccttcagg tggcgcagca ggtcaccgac gacctcgcac gcctcatcca tgggtatgcc
     3241 gtgcaggtcg cggtcggcgt cgaggcgctc gagcagcagg gcccacaggg acgggtctgc
     3301 tcgcaggagt cgaaccgccg cttggccacc ccagtgccgc aacgcgagat gctcgcccgc
     3361 ggcctcgtga tgcggccagg tgaccttgag catggccggg ccgtcggcgc tgacaaccgg
     3421 gagggcgatc gcgcaggccc cgtgccgggc cgggccgtcg ggcgtcaact cccaatcggc
     3481 gcacacgtcg gcaaccgtcc gcggcagccc gcgcaaccac gagtcgccgt cgatcgcgtg
     3541 tcgtgcggac gggccgtcca gcaggacatc cgccggacgc cccgccgccg cggcgacgag
     3601 accggcggga agcgccacca cggtcacccc agcgccagcc cgggcatcgt cggccaggcg
     3661 acgccgtggg cgacggccag cgttaccgct tcagtttcaa gccggaccac ttcgagcagg
     3721 gcagaggacc cctgcgggat ccgatcgaga tcgtccaacc cgcggtccac gaggttgcga
     3781 aggacgacgc tcaccagccg ccgagcgtcg tccggcccgg tgacggggaa gggcaggtga
     3841 taacccaaag caggaagcgg gtccggggtc agcgcggcgg ccagcgtcgc ctcgcggcgg
     3901 gtcagcgcgg cgaccgtggc gaggagcgac tcacggtccg tgtcgagcag atgcgctgca
     3961 gcgacctcga gggcgtattc gcaggctcgc gtcaccccca ccaacggcgt ggcgacgggg
     4021 gcgggcagcg ggtcgcgggt cggccagacg ggcggcgccg cgaggcttgc cgcgcccgca
     4081 gcgtcatggg cggcgatggc gacacccagg gctcgctggg tgacgaacga cccggcagcc
     4141 gcgatcgagg catcggcggt gtcgcgagcc gcgcgggcca actcccccgc ggtgaccggc
     4201 ggcggcaccg cgttcgtggg tgccgatgtg gacgccgtgg tcgcggttcc atcgacgggc
     4261 gcggtcggcc ccgaggtcgc cgtcgcggtc gagccgggcg gcccgctgat gattcgttcg
     4321 gggacctgcc ccgtttcgag gatcgcacgc agcacctggg cctgacgggt atgccgttgc
     4381 gtcacctcag gcgcgagagg cacccccgta gcccggccgg cgactccagc caacgccgtc
     4441 gcccgtcgat aggccgcgac gagcaacgcc tcgtcgggga tcggttggcg gctgacaaac
     4501 gggatggtgg gagcgtcgtc ctccaaccgg atcccacacc cggccaggcc gatgcccgcc
     4561 acggccgaca ggccggccaa ccccgccgcg cgcaacacgg tgcggcgggg cggcatacga
     4621 cggggtatgc cgcccaaacc ggcggtgagg ggggcccgag acacggcccg atcatggcat
     4681 gggacggtgc ccgcgccgct cgactgacgt cagggcagcg gtagcgccgt tacagtggtg
     4741 tcacccatcg gctggccgac gggtcccgcg agtgcggaaa atcgacaaga gcaggaggtg
     4801 tgcgccgtgc aggcaagcct tgtccgctcc attgtcgaag cgccgctggc cgcggtggat
     4861 ctggtcgtgg aggacgtcac cgtgaccgct gccgggcgtc gctcggtggt tcggatcgcg
     4921 gtcgaccgct ccctggccgg cctcgccaac actgacacca cctcgttcgt ggcgcctctg
     4981 tccttggacg aggtggccga tgccactcgg gtcgtcgatg cagccctcga ttccgacacc
     5041 cgcgtgggca acgcgcccta cgtgctcgag gtgagctcgc ccggcgtgga taggccgctg
     5101 acccagcctc gtcacctgcg ccgcaacgtc gggcgcctcg tgcggttgcg cctgaaggag
     5161 ggcgcggagc gctccgggcg gatcgcctcc gtgagcgatc tggtcatcgt cctgacaccg
     5221 gacccggctg ccgatccggc ggccaccgcc gcagggccgg tcgagatccc ggtggaccag
     5281 gtgcgcgacg gccgcgtcat catcgagttc gggcggatcg acgaggtcga cctggacgag
     5341 ccagacgacg agcttgacga gacagacgag acagacgann nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnga cgagacagac gagacagacg acgagttgga cgagccgaga
     5521 agtgaggagg gctgatggac atcgacctgg cagcgctacg ggcgctggaa cgcgaacgcg
     5581 acctcagcct tgaggtgttg atcccggcga tcgagcaggc cctgctgacc gcctatcacc
     5641 gcaccgatgg gtcgttcaag atcgcccgcg tggagttgga ccgcaagacc ggccacgtgg
     5701 ttgtcctcgc gcgcgaggag ggcccgctgc tgcccccgac cgaggagggc gaacgcccca
     5761 ctcgcggcga gcccggcccg gagttcgacg acacgccgga cggtttcggg cgagtcgctg
     5821 cggcgaccgc gcggcaggtc atcgtccagc gattgcgcga cctggaggac gaggcgatcc
     5881 tcggtgactt caagggtcgc gagggcgaca tcgtctccgg gatcgtccag caggggtcag
     5941 accctcgcaa cgtcctggtc gacttcggga cggtcgaagg catgctcccg caggccgagc
     6001 aggtgcccgg cgaggtctac cggcacggtg agcggctgcg ctgctacgtc gtcagcgtca
     6061 agcgtggtcc caagggcccg cagattgctc tgtcacgcac ccaccccaac ctcgtccggt
     6121 cgctgttcaa gcttgaggtg cccgagatcg cggacggcac cgtcgagatc gccgcgatga
     6181 tccgggaagc cggccaccgc accaagatcg cggtgcgctc ccgggtcccc ggggtcaacg
     6241 cgaagggggc atgcatcggc ccgatgggtg cccgggtccg ggccgtcatg aacgagttgg
     6301 gcggggagaa gatcgacatc gtcgactact ccgacgaccc gcgcgagttc atcgcggcgg
     6361 ccctgtcacc ggcgcgcgtg cagtcggtcg aggtcgtcga cccggtgctg cgtgccgccc
     6421 gggcaatcgt tcccgactac cagttgtcac tggcgatcgg caaggagggc cagaacgccc
     6481 gactggccgc caaactcacc ggttggcgga tcgacatccg ctccgacacc gctgtcgccg
     6541 agaaccctcc gacgtctgat gcgccgcctg ccagcgccac ccggccggcc ggtgaccgcc
     6601 gcctgcagca gggcggcggc ggacggcata ccggctcgcg cggcactgga cgcggtggct
     6661 ctggcgccgg tccgtccggc ccgccgcgcc gggggtgacc cacgggtcgc cccgccacgg
     6721 ggtagactga tggggaccga ccggactgga ctccgttcgg cgtccttccc gtatgccgcg
     6781 caacagggat cccctgtgcg cacctgcgtg ggatgtcgcc gcccggatag ctggttggtt
     6841 ctcgtgcgag tggtcgccat cgtggaagcc ggacaagccc ggttggtccc cgatgtgcga
     6901 catcggctcc ccgggcgcgg cgcatggctg cacccggaga ccgcttgtct agaggtcgcc
     6961 gtccggaaac gggcgttcgg aagagccctg cgcctgcagg tccccgccga cgccgcagcc
     7021 ctgacggcat acctgcgaga tctcgttcca ccgctcgagc cgacgatgtc cgtacaacca
     7081 caaggcgggt tagacgctga tgagcacgcg atgagcactc agcaatgagc aagccccagc
     7141 actaacgcgg tctgcgcccc tgtcgaggca cagacccaag acaggagaaa cgtggcaaag
     7201 gtccgtgtga gcgcgctcgc caaagagctc ggcaccgaca gtaagtccct gatggcgcac
     7261 ctcagtgcgc tgggggagta cgtcaagtcc ccgtcgtcga ccatcgagtc accggtcgaa
     7321 cgcaaggtcc gtgagacctt ccccaagccc gccgctgcgt cggctcccgc gcctgctgca
     7381 gctgcccccg ttgctcgtgc ggccaccccg gcggcacccg cagcaccggc ggcacccgcc
     7441 ccggtcaccc cgtcggctcc cgctccggcc gctgcggcgc ccgttgctcc cgcggcagct
     7501 gcacccgctc cggccccggc ccgctcgggc gtgccggccc cctcggcgcc ggctcccgcc
     7561 gcgccggcac cgtcggctcc cgcagcacct gctccggccg ctccggcggt ccaggctcct
     7621 gcggcccagg ctccggtcgc cgctccggca gcgccggtgg cgccgcgtcc ggccgcggca
     7681 ggtccgtcgg ctcccgcggc tccggctgcg ccgaccagcg caccgtccgg tgcgccctca
     7741 ggccccgtcc ccggcccgcg tccggctccg cgtccaggtt cgccgcgacc gggcaacaac
     7801 ccgttcgccc ccagccaggg catgggtcga cccccgcgtg aaggcggccc ggcgcgaccg
     7861 ggcaacaacc ccttcgcccc caaccagggg atgccgcgtc cgcaggcccg tccgggtgct
     7921 gctggccccg gcgggcctcg tccggcagcg gccggccccg gtgggccgcg tcccggtggt
     7981 ccgcgcccca accccgggat gatgcccgac cgtgcctcgg tcgcccgtcc cggcacccgt
     8041 ccggctcccg gtgctcgccc cggcggcccc ggtcgtcccg gtggcggtgg cggcggcttc
     8101 ggcggtcgtc ccggcggcgg cggtgcgccc ggtggtgctg gcgcaggtgg tggcggtggc
     8161 ttcggcggtc gtcccggtgc gcccggtcgt ggtggcaccc agggtgcctt cggtcgcggc
     8221 ggtggccgtc cggtgcgtgg gcgcaagtcc aagcgggcca agcgccagga attcgagcag
     8281 atgcaggccc cgacgatcgg tggcgtgagc gttccccgtg gggacggcac gaccgtcgtg
     8341 cgggtccggc gcgggtcgtc cttgaccgac ttcgccgaca aggtgaacgc caacccggca
     8401 tcgctggtca ccgtgctctt ccacctcggt gagatggcga ccgcgaccca gtcgctcgac
     8461 gaggacacct tcaagctcct cggcgccgag ctcggcttcg tcgtcgaggt cgtctcgccc
     8521 gaggacgagg agaaggagct cttcgcagcc ttcaacatcg acctcgacgc cgtcgaggac
     8581 gacgaggagc ttctcgtcgc tcggcccccg gtggtcacgg tcatgggtca cgtcgaccat
     8641 ggcaagacca agttgctcga cgccatccgg cacgccaacg tcatcgcgac tgaggccggc
     8701 ggcatcaccc agcacatcgg cgcctaccag atcgtcaccc agcacgaggg catcgatcgg
     8761 gcgatcacct tcatcgacac cccgggtcac gaggccttca cggccatgcg tgctcgtggt
     8821 gcgaaggtga ccgacatcgc catcctcgtc gtcgctgccg acgacggggt catgccccag
     8881 acgatcgagg cgctcaacca cgcgcaggcg gccgacgtgc cgatcgtcgt ggcggtcaac
     8941 aagatcgaca aagagggtgc caaccccgac aagattcgcc agcaactcac cgagtacaac
     9001 ctggtggctg aggagtacgg cggcgacacg atgttcgtcg acgtctcggc tcgcgagaag
     9061 ctcaacatcg aggggctgtt ggaagcggtg ctcctgacgg ccgatgccgc cctggatctt
     9121 cgggccaacc ccgacaccga tgctcgtggt gtggccatcg aggccaacct cgaccgtggc
     9181 cggggcgcca ccgccacggt cctggtccag cagggcacct tgcgggtcgg cgacgcgatc
     9241 gtcaccggct cggcccacgg ccgcgtccgg gccctgctcg acgagcacgg caacaacctc
     9301 gatgtcgcca ctccgtcgcg cccggtccag gtcttgggtc tgtcgtctgt gccgcgtgca
     9361 gg
//
LOCUS       DAOMZY010000115         9294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_7010_length_9294_cov_4.934300, whole genome shotgun sequence.
ACCESSION   DAOMZY010000115 DAOMZY010000000
VERSION     DAOMZY010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7010_length_9294_cov_4.934300"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PLK64_08000"
     CDS             <1..655
                     /locus_tag="PLK64_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPV79726.1"
                     /translation="ISVIHNGTDTPIGRIAARTAHPTIVVLGRLVPHKRVELAIDVLA
                     ALRDRHPGLRLRIVGDGWWREQVAAHAQAREVTDQVDLLGFVDETTKHRELASAWIAM
                     APSAKEGWGLNVVEAATHGVPTLAHHGAGGLSESILDGVTGILVHDTEGMVEAASSLL
                     SDEALRTRLGESARGHARTFTWAGTIQAWDQLLAHVASGGSPIAQIDEGLSRSALVS"
     gene            652..1410
                     /locus_tag="PLK64_08005"
     CDS             652..1410
                     /locus_tag="PLK64_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPV79727.1"
                     /translation="MTPRSTPALAATGSVSRSIQLFRAFRVEQSDPDFFYSLLARDSI
                     REVQRWCPLTGASVLDVGGGPGYFADAFTSAGARYAGVDPDAGELTARGGVAGNTMRA
                     SGLCLPFADGAFDVTYSSNVLEHVPDPEGMVAEMVRVTRPGGTVVVSWTPWLSPWGGH
                     ETSPWHYLGGVRAADRYAVRHGRRPKNDFGRSLFACSAARMLRWVRRAEEEGHLDLVD
                     MLPRYHPAWARWVVHVPGLREVVSWNVMLVVRPR"
     gene            1407..5597
                     /locus_tag="PLK64_08010"
     CDS             1407..5597
                     /locus_tag="PLK64_08010"
                     /inference="COORDINATES: protein motif:HMM:NF023275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-(1->3)-arabinofuranosyltransferase family
                     protein"
                     /protein_id="HPV79728.1"
                     /translation="MSGSGPVWRLRVATGCLLLSAMCFWQAPGLVVPDTKLDLTANPV
                     GLMARALDLWDDSAFGQLQNQAYGYLFPSGPFHALLSAAGVPAWIVQRLWWSLILGVA
                     FVGFWRLAGALRVGSPWSRYLGAALFALSPRFLSEVAVTSIEVWPMAVAPWVLTPLLD
                     PRPARMRARALRSALAFGCIGGVNAVATGAALILPVLWWVTRRPWRALLRPMLGWLGW
                     CLAAGLWWLLPLLLLGRYSPPFLDWIEDARVTTSTASPFNALQGTTPWLGYLAGPGGA
                     SWPAAYSLISQPTLIVVTGVVAVLGLSGLAHPRMPHRGWLAVSALVGLFLLTVGFSSA
                     ASGPLADAFHGLLDGPLAPLRNTHKFDLVLRIPIGLGLVHAASVLARWRPVRALRVEL
                     VAAVAGLLVVAAAAPAVAANLPRPGGYDGIPQYWHEAAAWLDDAEGQGAVLVLPSASF
                     ADFTWGSTRDEPLQALMQRPFVVRDAVPLGGAGSTRLLDEVTRRLTAGMGSPDLARVL
                     GEAGIRFLVVRNDLLPQSGVSRPVPLHEAIDESGFIRRASFGPPTGYLADAPGVTVDD
                     RMLVPYPSVEIFDGAAASAARFVPLDQVQLATAGPENLVDIAGSTPDGVVSILGSDRL
                     ALGDLAQGLPEVVTDGLRRRDVAFGSSGATTSEVLADGDPGTTGRRTIDYISDRTAAQ
                     TVTRWRGVRSVTASSSASDAGATLRLGPGTGPAAALDADPLTRWVSGTYGEAVGQWLE
                     LGLAAPQDISGLTIQVSGGYPIQAQPTRVRVSTDTGSVTTDIVETDSVVRLATPPGRT
                     TRVRVELDAVSSGAPNGFGIAELTVPGVHPVPELVIPAAAPAAPAQILSRVEVPALTG
                     CAFVATRSLCSPALVRSGEETAGIFRSLTLAAGAAYELSGTVLPGVGGNIERLLDFPG
                     RITATASSSRVQSPAARPATVADADLGTGWVAGDADPSPRLRLDLPQARTVTRLQFLL
                     DPYLAASRPTSVTVTGDDGTGSTVPVDAEGYAAISPTTGRGLDIDFLSDSGASSIDSV
                     SGFAAHLPVGVSEVRLEGADDLRAVLDPTRTVAVPCGFGPSVLVDDTRLPTQVEGTVE
                     QVLRGAPLRWSACPTTSAATVDLAAGRHTLSAAATTEFRPYQLLLRRVDAPPSAAAAS
                     TSASQSIAAAGDGGWAVPQRAQPGMLVLSHNANPGWVATDGQGQALPSVRVNGWQQGW
                     VLPAGAATVVHTRYAPQPWYAAGLAVGGVAFLALLVLWLLSTRPRGKPRNEIAESDGG
                     SVALSGALTGALTGSASGGWLLAVLLVVTFPVNAGLVGVLAVVIGAVVGYAGRRWPRL
                     GVTPVVVLGAGAVAAVAAAPWGEGGAALMSVGVQQLVLAAFAGVAASGTSGTVLPRWV
                     PSAGRPRRVSVRGGGPSA"
     gene            complement(5566..6732)
                     /locus_tag="PLK64_08015"
     CDS             complement(5566..6732)
                     /locus_tag="PLK64_08015"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPV79729.1"
                     /translation="MTDTLIDGPTQTRRTLRFPALDGLRIGGAVAVVSTHVGFTSGAS
                     LNGQQAGLLARLDAGVPLFFVVSGFLLYRPHVMAGLTQRPRPALGTYLRHRAARILPA
                     LWLAVLAAALLLHHAPLVGPSSFLRYATLTQIYTEVPAPTGLTQMWSLATEVAFYLAL
                     PALAWALDRSAPTPRAWVNRTLAVLLLLPVASAVWVAAATATGGGLPMLWLPAFLGWF
                     GLGMALAAWSGAREAGLLGPTVLDELGHAAGSVYAAAAALYLLLCTPIAGPYDLSPPT
                     VGQAVVKNLGYAVLGVLLVFPAVVGAAADRPSAIVRALGGRVGRALGDISYGVFAYHL
                     IVLALAERAFNHQTFDGQFVQLWVSTIAVTFVVAWVSYRVLERPIMRWARRLER"
     gene            complement(6729..8636)
                     /locus_tag="PLK64_08020"
     CDS             complement(6729..8636)
                     /locus_tag="PLK64_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79730.1"
                     /translation="MSHSGEPALAAVPPVMGPEGMPSEPGAAPSRSPSTTFRLGALAI
                     LVVTLVWRTWTVSAWSWIQDDWIYQIEAQRMPLGDYLTQNYNQHFMPGQFLLSWASTT
                     LAPLDYTVAIVVTVGLSALSILAWAAALAEVFGETSRALLALALVALSPIFLPTSLWW
                     AASIQVLPLQATMALVILFLCRYLRQPGVRPLVGLGIAYLLGLFFWQKALLLVIPAFF
                     LVLLLGSGSPLARLRSAWRPLGLLAALTALYVPVYLLVLAKPGSAGMGVDHTFTPRGL
                     GESLSFFFTGLFNIVLPSLVGGPWGAADNTTSIYDPIPAMLWLPMLALAVCAAILIVR
                     HRRRGGWLIGMVLTYAVVSWGLVLFSSRYQSIGVLSVREARYSADLLPVIFFALVFAV
                     TRSRIEAATATTPLRRPLPTVVTGRVDALVGLLTFAVSLSALAGNGAEWQRIEPHSPK
                     PWVTTIVRDATAVGPRSLFDGVAPDGVINPGFFPDYAHMSDMLSPLGLPLAFDAPARQ
                     VLIARGDGHLVNAAVEDLVQVPTGPVSDCGYLVKPGETAYVPVTKQLFAWTWFVQVDY
                     FSERGGTAVLRTDAKNWTIHLEPGLNQVQLPIVDSVSALAIAGAADAGTSCITKIQVG
                     NIRETRVLAAP"
     gene            8763..>9294
                     /locus_tag="PLK64_08025"
     CDS             8763..>9294
                     /locus_tag="PLK64_08025"
                     /inference="COORDINATES: protein motif:HMM:NF022715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin PorA family protein"
                     /protein_id="HPV79731.1"
                     /translation="MRRTLGAIALGLGALLLVSGFMAKPVLYTQLATVPLNYKSTSVS
                     VGRDMSVLRLWSDDAKVGHYDKLTGVTVQSTRQVLGIPGRVPADKQATTAFWQTGVKS
                     TAVGVGDLTYSMETVSFDRLTGVATNCCGDQVSIGDLEKPDAMKDITHEGGYFKFPFD
                     VQKQSYSWWDGDLGKAT"
BASE COUNT         1357 a         3194 c         3348 g         1395 t
ORIGIN      
        1 gatctcggtg atccacaacg ggaccgatac gccgatcggc cggatcgccg cccgcactgc
       61 gcatccgacg atcgtcgtgc tgggacgcct cgtgccacac aaacgcgtgg aactggccat
      121 cgacgtcctt gcggcgctgc gggatcgcca tcctggcctg cggctgcgca tcgtcggcga
      181 cgggtggtgg cgggagcagg tcgccgccca tgcgcaggcc cgcgaggtca ccgaccaggt
      241 cgatctgctc ggcttcgtcg atgagacgac caaacaccgc gagctcgcct cggcctggat
      301 cgcgatggca cccagcgcaa aggaagggtg gggcctcaac gtcgtcgagg cggcgaccca
      361 cggggtgccg acgctggccc atcacggtgc cggcgggttg tccgagtcga tcctcgacgg
      421 cgtcacgggc atcctcgtgc atgacaccga gggcatggtc gaggcagcct cctcgctgct
      481 cagcgacgag gcccttcgga cgcggttggg ggagagcgcc cgagggcacg cccgcacctt
      541 cacctgggcg ggaacgatcc aggcgtggga ccaactgctg gcgcacgtgg cgtccggggg
      601 ttcgcccatc gcgcagatcg acgagggcct gagccgctca gcgctcgtct catgaccccg
      661 cggtccacac ccgccttggc agccaccgga tcggtgtccc gctcgatcca gctcttccgg
      721 gccttccggg tcgaacagag cgatcccgac ttcttctact ccttgctcgc gcgggactcg
      781 atccgtgagg tccagcggtg gtgccctctc acaggtgcat cagtgctcga cgtcggtggc
      841 ggtccgggct acttcgcgga cgcgttcacc agcgccggtg cccggtatgc cggtgtggac
      901 cccgacgcgg gcgagttgac cgcgcgcggt ggcgtcgcgg gtaacaccat gcgcgcctcc
      961 ggcttgtgcc tgccgttcgc cgatggagcg ttcgacgtca cctactccag caacgtcctt
     1021 gagcatgtcc cggacccgga ggggatggtc gcggagatgg tccgggtgac caggcccggg
     1081 ggcacggtgg tcgtctcgtg gacgccgtgg ttgtcgccgt ggggcggtca tgagacctcc
     1141 ccgtggcact acctcggggg agtgcgagca gccgaccggt atgccgtgcg gcacggtcgg
     1201 cgcccgaaga acgacttcgg ccggtccctg ttcgcctgct cggccgcgcg gatgttgcgc
     1261 tgggttcgcc gggccgagga ggaggggcat ctcgacctcg tcgacatgct gccgcgctac
     1321 cacccggcct gggcccggtg ggttgtccac gttccaggtc tgcgcgaggt cgtcagctgg
     1381 aacgtcatgc tcgtcgtgcg tccccgatga gcggcagcgg tccggtctgg aggctgcggg
     1441 tggccaccgg ctgcctgctg ctctcggcga tgtgtttctg gcaggccccc gggctggtcg
     1501 tgcccgacac gaagctcgac ctcaccgcca acccggtcgg gctgatggcc cgggccctcg
     1561 acctctggga cgacagcgcg ttcggccaac tgcagaacca agcgtatggc tatctcttcc
     1621 ccagcggtcc cttccatgcc ctgctcagcg ccgccggggt cccggcctgg atcgtccagc
     1681 ggctgtggtg gtcgctgatc ctcggggtgg cctttgtggg cttctggcgg ctcgccggtg
     1741 ccctgcgggt cggcagcccg tggtcgcggt acctcggtgc agcgctgttc gccctatcgc
     1801 cccgctttct cagcgaggtg gcggtgacgt ccatcgaggt gtggccgatg gccgtcgcac
     1861 cctgggtgct gacgccactg ctggacccgc ggcccgcccg gatgcgggcc cgggccctgc
     1921 gctcggcgct cgcgttcggg tgcatcggcg gcgtcaacgc ggtggcgact ggcgcggccc
     1981 ttatcctgcc ggtgctctgg tgggtgaccc gacgaccctg gcgcgccctg ctgcgcccga
     2041 tgctcgggtg gctggggtgg tgtctggctg cgggcctgtg gtggttgctc cccctgctgc
     2101 tcctcggccg atacagccca ccattcctcg actggatcga ggacgcacga gtcaccacga
     2161 gcactgccag cccgttcaac gccctccagg gcaccacacc ctggctgggc tatctcgccg
     2221 gtccgggcgg ggccagttgg cccgcggcat actccttgat cagccagccg acgctcatcg
     2281 tcgtgacggg ggtcgtcgcg gtcctggggc tgtccggtct ggcgcacccg cggatgccgc
     2341 accgcggctg gctcgcggtg agcgccctcg tcggtctgtt cctcctcacc gtcgggtttt
     2401 cgtcggcggc atcggggccg ttggcggacg cattccatgg gctgctcgat ggcccgttgg
     2461 cgccactgcg gaacacccac aagttcgacc tggtcctgcg gatcccgatc ggccttggcc
     2521 tggttcacgc ggcgtcggtc ctcgcccgct ggcgtccggt gcgggcgcta cgtgtcgagc
     2581 tcgtggcggc ggtcgccggg ctgctcgtcg tcgcggcagc cgctcccgcc gtagccgcca
     2641 acctcccgcg gcccggcggg tacgacggca tcccgcagta ctggcacgag gccgccgcct
     2701 ggctcgacga cgctgagggc cagggcgccg tgctggtgct cccgtcggca tccttcgcgg
     2761 acttcacctg ggggtccacc cgcgacgaac cgcttcaggc cctcatgcaa cggcccttcg
     2821 tggtccggga cgcggtgccc ttgggcggcg cgggcagcac ccggctgctc gatgaggtga
     2881 cccgccgcct caccgcgggt atgggttcac cggacctggc tcgggtgctg ggggaggcgg
     2941 gcatccggtt cctcgtcgtg cgcaacgacc tcttgccgca gtccggggtg tcccggccgg
     3001 tgccattgca cgaggcgatc gacgagtccg gcttcatccg ccgggcgagt ttcggcccgc
     3061 cgaccggtta cctcgccgat gctcctgggg tgacggtgga cgaccggatg ctcgtgccct
     3121 atccgagcgt cgagatcttc gacggtgcgg cggcatcggc agctcggttc gtcccgctgg
     3181 atcaggtcca gctcgcgacg gccgggccgg agaacctcgt ggacatcgcc ggctcgacac
     3241 ctgacggggt tgtcagcatc ctcggatccg accgcttggc actgggggat ctggcccaag
     3301 ggctccccga ggtggtcacc gacggcctac gtcgccgcga cgtcgccttc ggcagctccg
     3361 gggcgacgac ctccgaggtc cttgcggacg gcgacccggg caccaccggc cgacgcacca
     3421 tcgactacat cagcgaccgg accgcggcgc agacggtgac gcggtggcgc ggggtccggt
     3481 ccgtgacggc gtcgtcgtcg gcttcggacg ccggcgcgac gctgcgcctg ggtccaggga
     3541 cgggcccggc ggccgcgctg gacgccgacc ccctcacccg gtgggtgagc ggtacgtacg
     3601 gcgaggcggt ggggcagtgg ctggaactcg ggctcgccgc accccaggac atctccgggc
     3661 tcacgatcca ggtgtctggc ggctacccga tccaggccca gcccacccgg gtgcgggtca
     3721 gcaccgacac cggctcggtc acgaccgaca tcgtcgagac ggacagtgtg gtccggctgg
     3781 cgacaccacc cggtaggacc acccgagtgc gggtcgagtt ggacgcggtc agctccggcg
     3841 cacccaacgg cttcgggatc gccgagctca ccgtccccgg cgttcacccc gtgcccgaac
     3901 tggtcattcc ggcggctgcc ccggctgccc cggcccagat cctcagccgc gtggaggtcc
     3961 ccgccctgac cgggtgcgcc ttcgtcgcga cccggtccct gtgctcgccc gccctcgtgc
     4021 gatccggtga ggagactgcc ggcatcttcc ggtcgttgac gcttgcggcc ggagcggcat
     4081 acgaactgtc cggcaccgtc ctgcccggcg tgggcggcaa tatcgaacga ctgttggact
     4141 tccctggccg gatcaccgcc acggccagca gtagccgcgt ccagagcccg gctgcgcggc
     4201 cggcgacagt cgccgacgcc gacctgggca ccgggtgggt ggccggcgac gccgatccga
     4261 gcccgcgact gcgactcgac ctcccgcagg ctcgcacggt gacccggctg cagttcctgc
     4321 tcgaccccta tctcgccgct tcccggccca cctcggtgac ggtgaccggc gacgacggca
     4381 ccggctcgac cgtgcccgtc gatgccgagg ggtatgccgc aatctcgccg accaccgggc
     4441 gcgggctgga catcgacttc ctgagcgaca gcggcgccag ctcgatcgac tccgtgagcg
     4501 gttttgccgc ccacctgccc gtcggggtgt cggaggtgcg gttggagggc gccgatgact
     4561 tgcgcgctgt cctggacccc acccgcaccg tcgctgtccc ctgtggcttc ggcccgagcg
     4621 tcctcgtcga tgacacacgg ctgccgaccc aggtcgaagg gaccgtcgag caggtgctcc
     4681 gtggtgcccc gctgcgctgg tcggcctgcc cgaccacgag cgccgccacc gtcgacctgg
     4741 cggcggggcg gcataccctc tcggcggccg ccacgacgga gttccggccc taccaactct
     4801 tgctgcgccg cgtcgatgcg ccgccgtccg ccgcggcggc ctcgacctcc gccagccaga
     4861 gcatcgcggc tgcgggcgac ggtgggtggg ccgtgccgca gcgcgcgcaa ccgggaatgc
     4921 tcgtcctcag ccacaacgcc aaccccggct gggtcgccac cgatgggcag gggcaggcgt
     4981 tgccctcggt ccgggtcaat ggctggcagc aggggtgggt gctccccgca ggagcagcca
     5041 ccgtggtgca cacgcgatac gccccccaac cgtggtatgc cgcgggcctc gccgtcgggg
     5101 gagtcgcctt ccttgccctg ctcgtcctgt ggctgctgag cacgcgccct cgcggaaagc
     5161 cccggaacga gatcgccgag tctgatggcg gatcggtagc gctgtccgga gcactgaccg
     5221 gagcactgac cggctcggcg tcgggcggat ggttgctggc ggttcttctg gtggtcacgt
     5281 ttcccgtcaa cgccgggctc gtcggagtcc tcgccgtggt gatcggcgcg gtggtgggct
     5341 acgcgggccg ccgctggccg cgtctgggcg tcaccccggt ggttgtgctc ggtgccgggg
     5401 cggttgccgc cgttgccgcg gccccgtggg gcgagggtgg ggccgcgctc atgtcggtgg
     5461 gggttcagca actcgtgctc gccgcgttcg caggcgtggc cgcctccggg acttctggga
     5521 cggttctccc acggtgggtg ccgagcgcgg ggcgccctcg ccgtgtcagc gttcgaggcg
     5581 gcgggcccag cgcatgatcg gtcgttcgag gacgcggtag ctgacccacg cgacgacgaa
     5641 ggtcaccgcg atggtgctca cccacaactg gacgaactgt ccgtcgaacg tctgatggtt
     5701 gaacgcccgc tcggcgaggg ctagcacgat gaggtgatag gcgaacaccc cgtaggagat
     5761 atcgcccaac gccctgccga cccggccgcc cagcgcgcgc acgatggccg aggggcggtc
     5821 cgcggctgcg cccaccacgg cgggaaacac gagaaggaca ccgagcacgg catacccgag
     5881 gttcttgacg acggcctggc ccacggtcgg tggcgacagg tcgtacggcc cggcgatagg
     5941 ggtgcacagc agcaggtaga gcgccgcggc cgcggcatac acgctgccgg cggcgtgccc
     6001 gagctcatcc aggaccgtcg gcccgagcag tcccgcctct cgcgcaccgg accacgctgc
     6061 taaggccatg ccgagcccga accaaccgag gaacgccgga agccacagca tcggcagccc
     6121 gccgccggtc gccgtcgcgg cggccaccca gaccgcggac gcgacgggga gcagtaacag
     6181 gacagcaaga gtccggttga cccaagcgcg gggggtcggc gcggagcggt cgagggccca
     6241 cgcgagggct gggagggcga ggtagaaggc cacctcggtc gccaggctcc acatctgggt
     6301 gaggccggtc ggggccggga cctcggtgta gatctgggtg agggtggcgt accgcaggaa
     6361 cgagctcggg cccacgagcg gggcgtggtg cagcaggagg gcggctgcga gcacggccag
     6421 ccacaacgcc ggaaggatgc gtgccgctcg gtggcgcagg taggtcccga gtgctggcct
     6481 cggccgctgc gtgagcccgg ccatgacgtg gggacggtag agcaggaagc cggacacgac
     6541 gaagaacagg gggaccccgg catcgagccg ggccagcagc ccggcctgct ggccgttcag
     6601 ggaggcaccc gaggtgaacc cgacgtgggt cgagacgacg gccactgcgc cgccgatgcg
     6661 cagcccgtcc agggccggga aacgcagggt ccggcgggtc tgggtgggcc cgtcgatgag
     6721 ggtgtcggtc atggggccgc caggactcgc gtctcgcgga tgttgcccac ctggatcttc
     6781 gtgatgcagg aggtgccggc gtcggcagct cccgcgatgg ccaaggcgga caccgagtcg
     6841 acgatcggta gttgcacctg gttgaggccg ggctcaaggt ggatcgtcca gttcttggcg
     6901 tccgtccgca gcaccgctgt gccgccccgc tccgagaagt agtcgacctg gacgaaccag
     6961 gtccaggcga acaactgctt cgtgaccggc acgtatgccg tctccccggg cttgacgagg
     7021 tacccgcagt cgctgaccgg gccggtgggg acctggacga ggtcctcgac ggcggcattg
     7081 acgaggtgac cgtcgccgcg ggcgatcagc acctgccggg cgggggcatc gaaagccagc
     7141 gggagcccaa ggggggacaa catgtcgctc atgtgcgcgt agtcggggaa gaaccccggg
     7201 ttgatcaccc cgtcgggcgc caccccgtca aagagcgacc gcggaccgac ggcggtggcg
     7261 tcgcgcacga tcgtggtcac ccagggtttc ggcgagtgcg gttcgatccg ctgccattcg
     7321 gcgccgttgc cggccagtgc tgacaacgag accgcgaagg tgagcaggcc gaccagtgca
     7381 tccacccgac cggtgacaac ggtggggagc gggcggcgca agggggtggt ggcggtcgcc
     7441 gcctcgatcc gggagcgggt gacggcgaag accagcgcga agaagatgac cgggagcagg
     7501 tcggcggagt agcgcgcctc ccgcacactg agcacaccga tcgactggta gcggctgctg
     7561 aacagcacca gcccccagga caccacggca tacgtgagca ccatcccgat gagccacccg
     7621 ccccggcggc ggtggcggac gatgaggatg gccgcgcaga cagccaacgc cagcatgggc
     7681 aaccagagca tggcgggaat cgggtcgtag atgctcgtcg tgttgtcggc ggcaccccat
     7741 ggcccaccca ccagcgacgg gaggacgatg ttgaacaacc cggtgaagaa gaaggacagc
     7801 gactcgccca gaccccgtgg cgtgaaggtg tggtcgacgc ccatgccggc cgacccgggt
     7861 ttggccaaga cgagcaggta gacgggcacg tagagcgccg tcagcgccgc gagaaggccc
     7921 aacgggcgcc aggccgagcg aagtcgcgcc agcggcgaac cggagccgag cagcaggacg
     7981 aggaagaagg ccgggatgac gagcagcagg gccttctgcc agaagaacag acccagcagg
     8041 taggcgatgc cgagcccgac caacggccgc accccgggct ggcgaaggta tcggcacaag
     8101 aagaggatga ccagcgccat cgtcgcctgc agggggagta cctggatgga tgccgcccac
     8161 cataggctgg tcggcaggaa gatcggcgaa agggctacca acgcaagggc cagcagggcc
     8221 cgtgaggtct cgccgaacac ttcggcgaga gcggccgccc aggccaggat cgacaacgcg
     8281 ctcaacccga ccgtgacgac gatggccacg gtgtagtcca ggggagccag ggtcgtcgag
     8341 gcccaggaca gaaggaactg gccgggcatg aagtgctggt tgtagttctg ggtgaggtag
     8401 tcgccgaggg gcatccgctg tgcctcgatc tggtagatcc agtcgtcctg gatccaactc
     8461 cacgcggaaa ccgtccacgt gcgccacacg agcgtcacca cgaggatggc gagcgcaccc
     8521 aaccggaagg tggttgacgg ggacctgctc ggcgcggcgc caggctccga cggcatacct
     8581 tccgggccca tgacaggagg gactgccgcg agggcgggct cgccactgtg cgacatgtca
     8641 cctcgatctg gccgaaaggg taggggaatg agggtgccgc tgttacgatc cgtcagattt
     8701 acgagcctac ccgtggggga cttggcaggc atcgacggtc actctagtgg ggagtaaaac
     8761 acgtgcgtcg cactctcgga gccatcgccc tcggccttgg cgccctcttg ctcgtctccg
     8821 ggttcatggc gaaacccgtc ctgtacacgc aactcgccac ggtgcccttg aactacaaga
     8881 gcacctcggt gtcggtcggc cgtgacatgt cggtcctgcg gctttggtcc gacgacgcca
     8941 aggttggtca ctacgacaag ttgaccggcg tcaccgtgca gagcacccgg caggtgctgg
     9001 gaatccccgg tcgggttcct gcggacaagc aggccaccac ggcgttctgg cagaccggtg
     9061 tcaagtcgac cgcggtcggc gtcggcgacc tcacctacag catggagacc gtgtcgttcg
     9121 accggctcac cggtgtggcc accaactgct gcggcgacca ggtctccatc ggcgacctgg
     9181 agaagcccga tgccatgaag gacatcaccc acgagggcgg ctacttcaag ttcccgttcg
     9241 acgtgcagaa gcagtcctac tcctggtggg acggtgacct gggcaaggca acgg
//
LOCUS       DAOMZY010000116         9276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_7028_length_9276_cov_6.279471, whole genome shotgun sequence.
ACCESSION   DAOMZY010000116 DAOMZY010000000
VERSION     DAOMZY010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9276
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7028_length_9276_cov_6.279471"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..1374
                     /gene="metK"
                     /locus_tag="PLK64_08030"
     CDS             157..1374
                     /gene="metK"
                     /locus_tag="PLK64_08030"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPV79732.1"
                     /translation="MSARLFTSESVTEGHPDKICDQISDAILDALLAQHPRSRVAVET
                     MVTTGLVHVAGEVTTEAYVEIPSIVRETVLAIGYDSSTKGFDGASCGVEVSIGQQSPD
                     IAQGVDTAYENRTGDGGLDVYDKQGAGDQGLMFGFACDDTAEFMPLPIHLAHRLAERL
                     TAVRKTQELPYLRPDGKTQVTIAYDGDRAVRLDTVVLSSQHAADVSIEGLLAPDIVAH
                     VIDPVLAELKDAGSDLDVSSYRALINPTGRFEVGGPMGDAGLTGRKIIVDTYGGMARH
                     GGGAFSGKDPSKVDRSAAYATRWVAKNVVAAGLARRCEVQVAYAIGAAHPVGLYVETF
                     GTETVPVDSIQRAIASVFDLRPAAIVDQLDLLRPIYQRTAAYGHFGRTDADGGFTWER
                     TDRVDALRAAVSR"
     gene            1445..2713
                     /locus_tag="PLK64_08035"
     CDS             1445..2713
                     /locus_tag="PLK64_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HPV79733.1"
                     /translation="MVSPLRRDEPGQPALLRSRGNVRPARQSSLREHNLSLVLRHIVD
                     AGTPMTRARLAKAVDLTRPAVSDLVDRLIAARLVVEAAPESVDRAGRPGVPLLPAPGS
                     FIGVGVDVAVDHIGVRALDLAGTLVTERIVEGDFRSSEPLAMLERVTSLTELVVAQLD
                     SLNATVAGVCLSVPGLTDPQGGIVRYAPNLGWREFDALAVLRRSARLRDLTLEVGNDA
                     NLAARAEARHRSRATGGSRLDQNFLFVSGEVGIGGAIVLGGDLATGLHGWSGEIGHSV
                     LDPAGPQCRCGATGCLEQYAGKDAILRRAHLPANTSVSRLLARARAGNSQATAALTEA
                     AEALGLASSSSLNLLDLDAVVLGGFYAQLFDVLGPLVADIINQRVLAARWVPVRVESA
                     VSGDYASLSGAALRVVDRVVAHPAPWLAPR"
     gene            2938..4467
                     /gene="gguA"
                     /locus_tag="PLK64_08040"
     CDS             2938..4467
                     /gene="gguA"
                     /locus_tag="PLK64_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018770239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPV79734.1"
                     /translation="MRNITKEFPGVKALENVSMVVRRGEVHAICGENGAGKSTLMKVL
                     SGVWPHGSYTGEIIYDGKPVEFKNIRQSEHAGIVIIHQELALIPELSIAENIFLGNEY
                     VKSGVIDWVETNRKARELLAHVGLNDEPQTAIKNIGVGKQQLVEIAKALNKDVRLLIL
                     DEPTAALNEDDSQHLLGLIDELRRKGITCIIISHKLNEIEQIADSITIIRDGQSVETL
                     DVKGDKVDEDRIIRGMVGREMSSRFPDYTPAIGDKFVEVRDWTVESPVIPGRMVCKGS
                     NFYVRRGEIVGFAGLMGAGRTELMRSIFGQSYGIFRSGELFLDGKPVSLRSVPAAIDA
                     GIGYVTEDRKVLGLNLLDDIKTTTVSAKLKKISKHSVVDRRAEFEAAEGYRKSLRTKT
                     PTVNEGVGKLSGGNQQKVVLAKWLFTDPELLILDEPTRGIDVGAKYEIYGIIQELAKS
                     GKAVIVVSSELPELLGLSDRIYTICEGAITGCLAKGEATQENLMKLMTTLSDRSAAAS
                     A"
     gene            4484..5722
                     /gene="gguB"
                     /locus_tag="PLK64_08045"
     CDS             4484..5722
                     /gene="gguB"
                     /locus_tag="PLK64_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPV79735.1"
                     /translation="MKKLKDIFGGDTRQFGMIFALVALIVFFQIMTNGLVLKPENVIN
                     IFNGNSYILVMAIGMVLVIIAGHIDLSVGSVAAAVGIVVALLMRDTGMHWVVAVLVGL
                     VVGAIIGAWQGFWVAYVGIPAFIVTLSGMMVFRGLNQLIGKSNTIPVGEDFQVIGAGY
                     LPEIGPNTGYNNLTVLLGLILVASVVFGAVRTRRNQVKIGAATDSATALWIRTGLISA
                     AILAVTLLMASGRKGTSFPVSGLILGALVLIYGFISTRTIIGRHVYAVGGNRHAAELS
                     GVRSKRVNFLVMMNMSILAALAGMMFVARSNASGPFDGVGWELDAIAAVFIGGAAVTG
                     GVGTVIGSIVGGLVMAVLNNGMQLLGVGADLTQIIKGLVLLIAVAFDVYNKMQGRPSI
                     IGLLMRNSKKAAAPVSDKAA"
     gene            5803..6897
                     /gene="chvE"
                     /locus_tag="PLK64_08050"
     CDS             5803..6897
                     /gene="chvE"
                     /locus_tag="PLK64_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPV79736.1"
                     /translation="MRTPIKSLLAVSAVAALALTGCGRSDSSTTATSGAAGAAAGAGT
                     LIGVALPQKTSENWVLAEQLFKDGLTAAGYKGDVQFANGGVSEQQNQIQSMVTKGAKV
                     IVVGAIDGSQLGTQLKAAKDGGATVIAYDRLIKNTAAVDYYVAYDNFKVGVLQGTALL
                     EGLKAKKPNGPYNIELFAGSPDDANAQVFFDGAMSVLTPKIADGTLKVASGQKDFTQA
                     VTPGWKAENAQKRMDTLLSANYSATDLDGVLSPNDTLARAILTSVKASGKASPVVTGQ
                     DSEVESVKSIMAGEQYSTINKDTRNLVKATIEMVTAIVKGGKPTVNDEKSYNNGVKVV
                     PANLLAPVIVTKANAKEAYANDPTLAQLVK"
     gene            7010..8245
                     /locus_tag="PLK64_08055"
     CDS             7010..8245
                     /locus_tag="PLK64_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HPV79737.1"
                     /translation="MSAEPTAAEACRLDRHASSLADLVARAVHPDGGFGWLGPDNEVV
                     LDRPVETWITCRMTHIAALEVMRRGDGAAHLRPLLDIGHDALTGRLRDHVSRGWLPAV
                     GGTAPQPTDKVAYAHAFVILAAASLTAAGHPGGPALLTEALDVFDTYFWDEDAGMSRE
                     QYDRAWTRCEDYRGVNANMHTVEAMLAAGEVLGDQRYIDRALRILRRVVHEFARDADW
                     RLPEHFDAQWQVLPDYNRDRPADQFRPFGVTIGHVLEWSRLALNARHTLGEAADAPQW
                     AFLREDAAAMFQAAVARGWDVDGAEGLVYTTDFQDVPIVRERLHWVVCEGIAAAWALH
                     EVTGDPDALAWYDRLWAYADRYLLDGVRGGWRHELSPTNQPSDAIWWGKPDLYHAYQC
                     TLVPLLPGLVSFAGAVRPE"
     gene            8287..>9276
                     /locus_tag="PLK64_08060"
     CDS             8287..>9276
                     /locus_tag="PLK64_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143997.1"
                     /note="binding of PriA to forked DNA starts the assembly
                     of the primosome, also possesses 3'-5' helicase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosome assembly protein PriA"
                     /protein_id="HPV79738.1"
                     /translation="MPIDDAPSPLPVAAVVVDSGLAHLDRPFEYAVPPELDAAAQPGV
                     RVRVRFAGRDLDGFVVARRAKAEHPGRLTPIRRVVSPEPVLTPQIHALTRAVADRYGG
                     VLGDVLRLAIPKRHATAERALPLDPPVPSPTEPSRTEKPTRAEAGPWAAYPAGEAFLR
                     RVAAGGAPGAAWLAIPGRAPEQDWPRAFAEAAAAALSAGRGAVIVVPDHRDVVRVDEA
                     LTAVLGAGRHVRLTADQGPQARYTAWLKLLRGHVRVVVGTRTAAFAPVRDLGLVAWWD
                     DGDDLLAEPRAPYPQVRTVLAIRAEQSAAALLVGGFGRSVQVQRWVAAGRVVDL"
BASE COUNT         1478 a         3183 c         3074 g         1541 t
ORIGIN      
        1 cgtcccgtcg tcacggtggg accggccacc aagtccgatg tcgccgcggc ggtgtgggac
       61 ggcatacagg ttctgcttcc cgccgcgcac tagactgaaa agccacgaca tcgcctgcgc
      121 ggtggttcct cagatcccca ccagctggag tcgagagtgt ccgcccgcct gttcacgtcc
      181 gagtcggtca ccgagggtca ccccgacaag atctgcgacc agatcagtga cgccatcctt
      241 gatgccctgc tggcccagca cccccgttcg cgcgtggccg tcgaaaccat ggtgacgacc
      301 ggcctcgtgc acgtcgccgg agaggtgacg accgaggctt acgtcgagat tccgtccatc
      361 gtccgtgaga cggtgctggc gatcggttac gactcatcga ccaagggctt cgacggagcg
      421 tcgtgcggcg tcgaggtgtc gatcggccag cagagccccg acatcgccca aggcgtcgac
      481 accgcctatg agaaccgcac cggtgacggc gggctggatg tctacgacaa gcagggtgcc
      541 ggcgaccagg gcctgatgtt cgggttcgcg tgcgacgaca ccgcggagtt catgcccctg
      601 ccgatccacc tggcccaccg gctggccgag cggctgacgg ccgtgcgcaa gacgcaggag
      661 ttgccctacc tgcgtcccga cggcaagacc caggtgacta tcgcctacga cggcgaccgg
      721 gcggttcggc tggacaccgt ggtgttgtcc agccagcacg cggccgacgt gtcgatcgaa
      781 gggctgttgg ctcccgacat cgtggcgcac gtgatcgacc cagtgctcgc cgagctcaag
      841 gacgccggca gcgacctgga cgtgtcgtcc taccgcgccc tcatcaaccc caccggtcgg
      901 tttgaggtcg gcggcccgat gggcgatgca ggcctcaccg gtcgcaagat catcgtcgac
      961 acctacggtg ggatggcgcg gcacggtggc ggcgcgttct ccggcaagga cccctcgaag
     1021 gtcgaccgct cggcggcata cgccacgcgg tgggtagcca agaacgtcgt ggctgccggg
     1081 ctggcccggc gctgcgaggt gcaggtggcc tacgccatcg gagccgctca cccggtgggt
     1141 ctgtatgtcg agacgttcgg caccgagacg gtcccggtcg actcgatcca gcgggccatc
     1201 gcgtcggtct tcgacctgcg tccggccgcg atcgtggacc agttggatct gctgcgcccg
     1261 atctatcagc ggaccgcggc ctacggtcac ttcgggcgaa ccgacgcgga cgggggcttc
     1321 acctgggagc gcaccgatcg ggtcgacgcc ttgcgcgcgg ccgtcagccg ctgatcgagc
     1381 cgtccatcgt ttgtttgttc cggggacgaa caaaatcggg gtatcgcgcg gtatggtcgg
     1441 ccccatggtg agccctcttc gacgcgatga gccgggtcag ccggctctgc tgcgcagccg
     1501 aggtaacgtc cgccctgcgc ggcagtcctc acttcgtgaa cacaaccttt cgctcgtcct
     1561 gcgccacatc gtcgacgcgg ggacgccgat gacacgcgcc cggttggcga aggcggtcga
     1621 cctcacccgt ccggcggtgt ccgatctggt cgaccgattg atcgctgcgc gactggtggt
     1681 cgaagcggca cccgagagcg tcgatcgggc tgggcgcccg ggtgtccctt tgctgcccgc
     1741 gcccggatcg ttcatcgggg tcggtgtcga cgtcgccgtc gatcacatcg gtgtccgtgc
     1801 actcgacctg gccgggaccc tagtcaccga gcggatcgtc gaaggcgact tccggtcgag
     1861 tgaaccgctc gccatgctcg agcgagtgac atcgcttacc gaacttgttg tcgcccaact
     1921 ggattcactc aacgcgacag ttgccggggt gtgtttgtcg gtccccggtc tgaccgatcc
     1981 gcagggcggg atcgtccgct atgcccccaa cctgggctgg cgagagttcg atgcgctcgc
     2041 ggtgctccga cgcagcgccc ggttgcgtga tctcacgctc gaggtgggta atgacgccaa
     2101 cctggccgcg cgcgccgagg cgcggcaccg gtcccgggcg acgggagggt cgcggctgga
     2161 ccagaacttc ctcttcgtca gtggtgaggt cggcatcggc ggcgcgatcg ttctgggcgg
     2221 cgatcttgcc actggcctgc acgggtggag cggcgagatc gggcacagcg tcctcgaccc
     2281 tgcgggtccg caatgtcgtt gtggggcaac aggttgcctg gagcagtatg ccggcaagga
     2341 cgccattctg cgccgagcgc acctcccggc caacacgtcg gtgtcgcgcc tgctggctcg
     2401 ggcgcgggcc gggaacagcc aggcgaccgc tgcgctcacc gaggccgccg aggcgctggg
     2461 actcgcgtcc tcctcctcgc tcaacctgct ggacctggac gcagtcgtgc tcggcggctt
     2521 ctatgcccaa ctgttcgacg tcctcggtcc gttggttgcc gacatcatca accagcgagt
     2581 gttggccgcg cggtgggtgc cggtgcgggt ggagtcggcc gtcagcggcg actacgcctc
     2641 cttgagtggc gcggcgctgc gtgtcgtcga ccgcgtcgtt gcccacccgg cgccctggct
     2701 ggccccgagg tgagcccctg ccggcccgtc gtttggtgat cgaatcgtta cccaccgcgg
     2761 cgcggggcgg ccttgacgag ccattgtccc agcaataagt tgatcgggat aacaaatagc
     2821 tctaggtggc ttgggcgtgg ccgagtaggt taggggccgc gccagtgccg tcacggcagg
     2881 cgctcggtgc tgagcgacgt gggaaggtgg caactggtgt cagacgtcat cctcgagatg
     2941 cgcaacatca ccaaggagtt ccccggggtc aaggcccttg agaacgtatc catggtggtc
     3001 cgccggggcg aggtgcatgc catctgcgga gagaacggtg caggcaaatc gaccctcatg
     3061 aaagtgctgt cgggggtgtg gccgcacggg agctataccg gcgagatcat ctacgacggg
     3121 aagccggtcg agttcaagaa catccggcag tcagaacatg ccgggatcgt gatcatccac
     3181 caggagctcg cgctcatccc tgagttgagc atcgcggaga acatcttcct gggcaacgag
     3241 tacgtcaaga gcggtgtcat cgactgggtc gagaccaacc gcaaggcacg cgagctcctc
     3301 gcgcacgtcg gcctcaacga cgagccccag accgcgatca agaacatcgg cgtcggcaag
     3361 cagcaactcg tcgagatcgc gaaggcactc aacaaggacg tccggctgct catcctcgat
     3421 gagcccaccg ccgctctcaa cgaggacgac tcgcagcacc tgctcggtct catcgacgaa
     3481 ctgcgccgca agggcatcac ctgcatcatc atcagccaca agctgaacga gatcgagcag
     3541 atcgccgact cgatcaccat catccgcgac ggccagtcgg tcgagacgct cgacgtcaag
     3601 ggcgacaagg tcgacgagga ccgcatcatc cgcgggatgg tcgggcgcga gatgagctcg
     3661 cggttcccgg actacacccc cgccatcggc gacaagttcg tggaggtccg cgactggacg
     3721 gtggagagcc cggtcatccc ggggcgcatg gtgtgcaagg gctccaactt ctacgtccgt
     3781 cgcggtgaga tcgtcggctt cgccgggctc atgggtgccg gtcgtaccga gctcatgcgg
     3841 tcgatcttcg gtcagtccta tggcatcttc cgctccggcg agctgttcct cgacggcaag
     3901 ccggtcagcc tgaggagcgt cccggcagcg atcgacgccg gtatcggcta cgtcaccgag
     3961 gaccgcaagg tgctgggcct gaacctgctg gacgacatca agacgactac ggtgtcggcg
     4021 aagctgaaga agatctccaa gcactcggtg gtcgaccgtc gggccgagtt cgaagcggcc
     4081 gagggctacc gcaagtcgct tcgcaccaag acccccacgg tcaacgaagg ggtcggcaag
     4141 ctctccggcg gcaaccagca gaaggtcgtc ctggccaagt ggctgttcac cgaccctgag
     4201 ctgctcatcc tcgacgagcc gacgcgtggc atcgacgtgg gcgccaagta cgagatctac
     4261 gggatcatcc aggaactcgc caagagcggg aaggcggtca tcgtcgtctc ctcggaactt
     4321 cctgagctcc tgggcctctc cgaccggatc tacacgatct gcgagggcgc catcacgggg
     4381 tgtctggcca agggcgaggc cacccaagag aacctcatga agttgatgac caccctttcc
     4441 gaccgctccg ccgccgcgtc agcctgacac ccaaggactc ttgatgaaga aactcaagga
     4501 catcttcggc ggagacaccc gccagttcgg catgatcttc gcgctcgtcg cgctcatcgt
     4561 gttcttccag atcatgacca acgggctcgt cctcaagccc gagaacgtca tcaacatctt
     4621 caacggcaac tcctacatcc tcgtcatggc gatcgggatg gtcctggtga tcatcgccgg
     4681 ccacatcgac ctgtcggtcg gctcggtggc ggccgcggtc ggcatcgtcg tggcactgtt
     4741 gatgcgtgac accgggatgc actgggttgt cgccgtgctc gtcgggctgg tcgtgggggc
     4801 gatcatcggg gcgtggcagg gcttctgggt cgcctacgtg ggcatcccgg ccttcatcgt
     4861 caccctgtcg ggaatgatgg tcttccgcgg gctcaaccag ctcatcggta agtcgaacac
     4921 catcccggtc ggtgaggact tccaggtcat cggtgccggc tacctgcccg agatcgggcc
     4981 gaacacgggc tacaacaacc tcaccgtcct gttgggcctg atcctcgtcg cgtcggtcgt
     5041 cttcggtgcc gtccgcaccc gccgcaacca ggtcaagatc ggcgcggcca ccgactcggc
     5101 cacggcgctg tggatccgga cgggcttgat cagcgcggcc atcctggcgg tcaccctcct
     5161 catggcctca ggccgtaagg gcacgtcgtt ccccgtgtcg ggcctcatcc tcggtgcgtt
     5221 ggtgctcatc tacggcttca tctcgacccg cacgatcatc ggtcggcacg tctacgcagt
     5281 cggcggcaac cgccacgcgg ctgagctctc cggcgttcgc agcaagcgcg tcaacttcct
     5341 cgtcatgatg aacatgtcca tcctcgccgc cctggccggg atgatgttcg tcgctcgctc
     5401 caacgcctcc ggtccgttcg acggggtcgg ctgggagctt gacgcgatcg ccgccgtctt
     5461 catcggtggc gccgccgtca ccggtggtgt gggcacggtc atcgggtcga tcgtcggtgg
     5521 tctggtcatg gccgtcctca acaacggcat gcagttgctc ggtgtcgggg ccgacctcac
     5581 ccagatcatc aagggcctgg tgctcctcat cgccgttgcc ttcgacgtct acaacaagat
     5641 gcaggggcgc ccgtcgatca tcgggctgct gatgcgcaac tccaagaagg ccgcggcgcc
     5701 ggtatccgac aaggcggcct gaccttcatc cacgcgcgac cgtcccggtc ctcgcgtacg
     5761 tctcacctcg aattagtctc accaaccaga gagagaaaca gaatgcgcac acccatcaag
     5821 tcccttctgg cggtcagcgc tgttgccgcc ctggccctca ccggctgcgg ccggtccgac
     5881 agcagcacca cggccacctc gggtgccgcc ggtgcggccg cgggcgccgg caccctcatc
     5941 ggtgttgccc tcccgcagaa gacgtcggag aactgggtgc tcgccgagca gctgttcaag
     6001 gacgggctga cggctgccgg ctacaagggt gacgtccagt tcgccaacgg cggcgtctcg
     6061 gagcagcaga accagatcca gtcgatggtc accaagggcg ccaaggtcat tgtcgtcggc
     6121 gcgatcgacg gctcgcagct gggcacccag ctcaaggctg ccaaggacgg cggcgcgacg
     6181 gtcatcgcgt atgaccgcct catcaagaac accgcggcgg tcgactacta cgtcgcctac
     6241 gacaacttca aggtcggtgt gctccagggc accgcgctcc tcgaggggct caaggccaag
     6301 aagcccaatg gcccctacaa catcgagctg ttcgcgggtt cgcctgacga cgccaacgcc
     6361 caggtcttct tcgatggcgc catgagcgtc ctcaccccga agatcgccga cggcaccctc
     6421 aaggtcgcct cgggtcagaa ggactttacc caggccgtca ccccgggttg gaaggccgag
     6481 aacgcccaga agcgcatgga caccctgctg tcggccaact actcggcgac cgacctcgac
     6541 ggcgtcctgt ccccgaacga caccctggcc cgtgcgatcc tcacgtcggt caaggcctcc
     6601 ggcaaggcgt ccccggtcgt caccggtcag gactccgagg tcgagtcggt caagtcgatc
     6661 atggccggcg agcagtactc cacgatcaac aaggacaccc gcaacctcgt caaggcgacc
     6721 atcgagatgg tcaccgcgat cgtcaagggt ggcaagccca cggtcaacga cgagaagtcc
     6781 tacaacaacg gcgtcaaggt cgttccggcc aacctgctgg ctccggtcat cgtgaccaag
     6841 gccaacgcca aggaggctta cgccaacgac ccgaccctcg cgcagttggt caagtgacct
     6901 gcgtctgacc tgacagtcgg ttaaaccgaa cggtgggtcc agtgctccgc ggggagcgct
     6961 ggacccaccg cgtcgtttcc cgaacgttcc accgacaagg agagccagca tgagcgccga
     7021 accgaccgca gccgaggcct gtcgcctgga ccgccacgcg agcagtctgg ccgacctcgt
     7081 ggctcgcgcc gtccatcccg atggcggctt cggctggctg gggccggaca acgaggtcgt
     7141 cctcgatcgc ccggtcgaga cctggatcac ctgccggatg acccacatcg cggcccttga
     7201 ggtgatgcgc cgtggcgatg gcgcggcgca cctgcgcccc ttgctcgaca tcggacacga
     7261 cgcgctgacc ggccgccttc gcgaccatgt gtcacggggt tggcttcctg cagtaggcgg
     7321 cacggcaccg cagcccaccg acaaggtggc ctatgcccac gccttcgtca ttctggccgc
     7381 agcgagcctg acggccgccg gtcaccccgg ggggccggcc ctgctgaccg aggccctcga
     7441 cgtgttcgac acttacttct gggacgagga cgcgggcatg tcccgcgagc agtacgaccg
     7501 cgcctggacc cggtgtgagg actaccgcgg cgtcaacgcc aacatgcaca ccgtcgaggc
     7561 gatgctcgcc gcgggggagg tgctcggcga tcagcgctac atcgaccgcg cgctgcggat
     7621 cctccgtcga gtcgtccacg agttcgcccg cgacgcggac tggcgcctgc ctgagcactt
     7681 cgacgcccaa tggcaggtgc tgcccgacta caaccgcgac cgtcccgccg atcagttccg
     7741 ccccttcggc gtgacaatcg gccacgtcct ggagtggtcg cggctggccc tcaacgcgcg
     7801 gcataccctc ggggaagccg cggacgcgcc gcagtgggcg ttcctgcggg aggacgccgc
     7861 tgcgatgttc caggccgcgg tcgctcgggg ctgggacgtc gatggagccg aggggctcgt
     7921 ctacacgacc gacttccagg acgtcccgat cgtgcgggag cgcctgcact gggtggtctg
     7981 cgagggcatc gcggccgcgt gggcgctgca cgaggtcacc ggtgatccgg acgctctggc
     8041 gtggtacgac cggttgtggg cgtatgccga tcgctacctc ctcgacgggg tccgcggagg
     8101 ctggcgacac gaactctcgc cgaccaacca accctccgac gcgatctggt ggggtaagcc
     8161 ggacctgtac cacgcctacc agtgcaccct tgtcccgttg ttgcccggcc tggtctcgtt
     8221 cgcgggagcc gtgcggccgg agtgaccgcg cgaatcggtg tctgccctgc gggttagggt
     8281 gccagcgtgc ctatcgacga cgcccccagc cccctcccgg tggcagccgt tgtcgtcgac
     8341 agtgggctcg cccacctgga ccgtccgttc gagtatgccg tcccgcccga gttggacgcc
     8401 gccgcccaac cgggggtgcg ggtcagggtc cgcttcgccg ggcgggatct ggacggcttc
     8461 gtcgtcgcgc gccgagcgaa ggccgagcac cccggccggc tcacgccgat ccgtcgggtc
     8521 gtgagccccg aacccgtcct caccccgcag atccacgccc tcaccagggc ggtcgccgac
     8581 cgctacggcg gggtcctcgg cgacgtgctg cgcctcgcga tccccaagcg tcatgccacc
     8641 gctgaacgcg cgctgcccct cgacccaccc gtgccatcgc cgaccgagcc ttcgagaacc
     8701 gagaagccga cccgcgccga ggccgggccg tgggcggcat acccggcggg tgaggcgttc
     8761 ctgcgtcggg tcgcggcggg cggcgctcca ggggctgcct ggctggccat ccctggccgt
     8821 gcccccgagc aggactggcc gcgagcgttt gccgaggcgg cggcggccgc gctgtcggcc
     8881 ggccgtgggg cggtgatcgt cgtccccgac caccgcgatg tcgtccgggt cgacgaggcg
     8941 ctcactgcgg tcctcggggc tggtcggcac gttcgcctca ccgccgacca agggccgcag
     9001 gcgcgttaca cggcctggct caagctcctg cgggggcacg tgcgcgtcgt cgtcggcacc
     9061 cggactgcgg ccttcgcgcc cgtgcgcgat ctgggcctgg tcgcgtggtg ggacgacggt
     9121 gacgatctgc tcgccgagcc ccgagcgccc tacccgcagg tgcggaccgt ccttgccatt
     9181 cgggccgagc agtcggccgc agcgctgctg gtcggcgggt tcggccggtc ggtccaggtg
     9241 cagcgctggg tggcggccgg gcgagtggtg gacctg
//
LOCUS       DAOMZY010000117         9264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_7041_length_9264_cov_6.550657, whole genome shotgun sequence.
ACCESSION   DAOMZY010000117 DAOMZY010000000
VERSION     DAOMZY010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9264
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7041_length_9264_cov_6.550657"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="PLK64_08065"
     CDS             complement(<1..739)
                     /locus_tag="PLK64_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79739.1"
                     /translation="MSRPNPAAAPLAAEQREPTKAFLWIYVLAYFGGWLGILGPAILA
                     LYTRLADLFPPAGQGTTAVSALAVVSGVGAVAATLANPFIGRLSDRTTSRFGMRTPWL
                     IISSVIFVLGSLVVAFGPANIAVVTIGWSLSQVGFNGMLAIFTAVLPDQIPERMMGRV
                     SSVLGIAMNVASLAAVWLVGLFAAGTITFDDAGVITGGDPSSPLRFIVPVAVAVATSM
                     VLVVALRPIDRHIQRSQVPPYSVAEFFG"
     gene            819..1427
                     /locus_tag="PLK64_08070"
     CDS             819..1427
                     /locus_tag="PLK64_08070"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV79740.1"
                     /translation="MTEDSRRRVGRPADEPDARDRIMAAAIAEFSRNGFNGTSLERIA
                     QGAGLSKAGVLHHFGSKAAIPFAFLGDRDDVGIRLTENESDFPDVLTSLVTVARRDVA
                     ASTETRVYAVLSGEAVGIDSPLRPWFQQRYAALQRQVAGGLRRALDGGRLRAGVDVDA
                     AAAEALAVMDGLQLQYLLDDDADTYLARFAAYIDRLVVSLSA"
     gene            1531..3771
                     /locus_tag="PLK64_08075"
     CDS             1531..3771
                     /locus_tag="PLK64_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPV79741.1"
                     /translation="MTEALLERRLSLIVEHFPIAVLLETVDRKVSQTNQAFCDMFGIP
                     APPAALVGADCEAAAVQLAPLWGDLDGFLARVHELLAAGKPVVGDRIELTDGRVLERD
                     FLMVPVDETRGEAAWIYRDVTVSDTARRVAQSEANARSELLATISHDVRTPVVGIVGL
                     VDILLQQPLDHRTRELVESVHSSAAAMTTMLDDLLDLSRADAGRLELCIEDTSICELV
                     EDVAGMVGPVAQAKSLPLIAGVKATVPDIIRTDPGRLRQVLLNLTSNAVKFSPTGAVT
                     ILADREGSDLVVRVSDTGPGMTPKAITRAFEQYVQGGAEVNRAYGGAGLGLAIANKLT
                     AALGGTITVHSRIGLGTTFAVRIPGAVVGPELSTTMTGIRAHLTGHHRAVPVVAAALE
                     RAGIEVRDQADDEGVNLEVVVASSLAHAQRIPAASRRQLILVPAALASCPPLAGTALA
                     LPWTRERLRAALRDEWVADDVRERAGLLPLGTRVLLAEDEPSNRRIISEMLTRLRAEV
                     VAVGTGLEALEALAADRFDVVLMDLTMPVMGGVEAVENIRHRVPADRCPPILALTADA
                     RVDPSLRPDSGFCGHLSKPVTSAMLSRAVAGVLKASSPEPPPTLGERPAVDVTVLHAL
                     VEDVGDTQVVLDTIELYLEELPVRLSSMTAALTGERFDLLRDEAHALKSSSRMLGAGT
                     LADQCRDLEAVTAAHSRQDSASGGTVTPMVDALQFEAFRVAQWLADFRAASYPGLDVP
                     SASSGT"
     gene            complement(3729..5480)
                     /locus_tag="PLK64_08080"
     CDS             complement(3729..5480)
                     /locus_tag="PLK64_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPV79742.1"
                     /translation="MSEQHPADAELRRQLARAEARLKRERAARRESEDIAEATLRRLY
                     LRQADVDLLSQVTSIANSAQEFESAFLDAMVLIRRASQWQVAHYFLPAKDDPTVMVSS
                     GVWSGEPDDPFLDELRSATSGMRFPPGSGIPGEAYLQGATWEEDIAASRNFPRQQWLT
                     GGSAFAFPILIGKEVVAVMEFLRSSPRRPQQGLLEIADVIGIQLGRVVERARARQSES
                     GHRTSLQDVVAQRTADLIAARNRAEAQSDARSTLFSTVSHDLQTPLHAALADIEAAAS
                     APDSGEHLARAVSHLIELGQRIRALVELAAPTDANADHPRVVDLSRVLDDVIAAHDAA
                     RRHRDTSLDVAVVFAQSDEVLVDVERFRRIIYTLLAGRLIEQSAGPVRIRLEVRATWA
                     DLTLDEPDPGSEGTTLGLARQLAHAAGGEICVHALPDRRRRIEVRIPVAVQGRHRRGI
                     GRRILLVDDIAVTRQISAAMLTRMGAVVDTAENGQQALDVLREHEYALVLMDLRMPIM
                     GGLEASRLIRAGEAGGERCDVPVVALTAHSAPGDADRSLLAGMDAHLTKPFTYDELRA
                     TVLLYVPELAEGTSRPG"
     gene            complement(5477..6025)
                     /locus_tag="PLK64_08085"
     CDS             complement(5477..6025)
                     /locus_tag="PLK64_08085"
                     /inference="COORDINATES: protein motif:HMM:NF019320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HPV79743.1"
                     /translation="MKGVIFNVIEETVRAEHGEDAWDELLDTAGLDGAYTSLGTYPDH
                     EMAVLVQTASAALGLSQEELLRHLGRVGFSRLAARNADLMIDFKDARSLLLGLNSVIH
                     PEVRKVYPGADVPVFDVVSGQGHRMSLSYRSNRGMCYLGEGLALGAADHFGETMVVTQ
                     PICQHRGGDHCELQFIWPEARR"
     gene            complement(6780..7625)
                     /locus_tag="PLK64_08090"
     CDS             complement(6780..7625)
                     /locus_tag="PLK64_08090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HPV79744.1"
                     /translation="MKISVIVPTRNSGRTLAACLGSLRRQTHEDVEIIVVDNASTDDT
                     PPIALQLAHIIVDRGPERSAQRNRGAWLASGEIVVFIDSDMVLEPTVLADLAATFEAR
                     PEVGAVIIPQRSFGVGFLASCRVLEKSLHVGLDDVEAPRAFRTEVFDLVGRWDETLSA
                     AEDWDLADRTRAAGVEIARVSSWIWHDEGKISLRDQFAKKRYDGRWVAEYLSRKPETR
                     QHVSRPGLRSQLPALLASPVRTGGLVILKSVETAGLVAGMSKAQRAGLVSPRRVSRGP
                     AGLCV"
     gene            complement(7622..7816)
                     /locus_tag="PLK64_08095"
     CDS             complement(7622..7816)
                     /locus_tag="PLK64_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79745.1"
                     /translation="MTTLTLSPALAIAPMALAPSAFAALAAVLVCTLRYAVLLPAPRG
                     RDERRNCDPIAYGDIPEEIS"
     gene            complement(8305..8997)
                     /locus_tag="PLK64_08100"
     CDS             complement(8305..8997)
                     /locus_tag="PLK64_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPV79746.1"
                     /translation="MVAPVALLIEDSKEYALIGAKLLQREGFNVLVAHDGETGLGLAR
                     ARKPELVLLDIGLPGVDGFEVCRRLRDFSDAYLIMVTGRTDEVDRVIGLTMGADDYVT
                     KPFSARELAARIAAMRRRPRVVPDPELWDFGPLRIDVAARETTLDGELLDLTKVEFDL
                     LAKLASEPRRAFTRSQLLDAVWGVDWFSDDHVVDVHVGNLRKKLGESGSRPRFVHTVR
                     GVGFRFEPNSKR"
BASE COUNT         1451 a         3108 c         3274 g         1431 t
ORIGIN      
        1 acccgaagaa ctcggccacg ctgtagggcg gcacctggga gcgctggatg tgccggtcga
       61 tcgggcgcag ggccacgacg aggaccatcg aggtggcgac ggcgacggcc acggggacaa
      121 tgaaccgcaa ggggctcgac gggtcgcctc cggtgatgac gccagcgtcg tcgaaggtaa
      181 tggtgcctgc ggcgaagagg ccgaccaacc agacggcagc gaggctggcg acgttcatcg
      241 cgatgcccag caccgaggac acccggccca tcatccgctc agggatctgg tcgggaagca
      301 cggcggtgaa gatggccagc atgccgttga agcccacctg cgagagcgac cagccgatcg
      361 tgacgacggc gatgttggcc ggtccgaagg ccacgacgag cgagccgagg acgaagatca
      421 ccgagctgat gatcagccag ggggtccgca tcccgaagcg cgaggtggtg cggtcactca
      481 ggcgcccgat gaaggggttg gccagggtgg ccgccacggc cccgacgccg gagaccaccg
      541 cgagcgcgga gaccgcggtc gtgccctggc cggccggcgg gaagaggtcg gcaaggcggg
      601 tgtagagggc gaggatggcc ggtccgagga tccccagcca gccgccgaag tacgccagga
      661 catagatcca caggaaggcc ttggtcggct cgcgctgctc ggcggcgagc ggcgcggcag
      721 cggggttcgg tcgggacatc gttgtctcct tgaagggggt gatggcgcat actacccacc
      781 aaccggttgg taagcgcaag accctttatg ctcgggtgat gaccgaggac tcccgccgcc
      841 gggtcgggcg tccggccgac gagcccgacg cccgagaccg gatcatggca gcggcgatcg
      901 cggagttctc ccgcaacggt ttcaacggca cctcactgga gcggatcgcg cagggcgcgg
      961 gtctctccaa ggcgggtgtg ttgcaccact tcggcagcaa ggccgcgatc ccgttcgcct
     1021 tcctcggaga ccgcgacgac gtgggaatcc ggttgactga gaacgaatcc gacttcccgg
     1081 acgtgttgac cagcctcgtc acggtcgccc ggcgcgatgt cgcggcctcc accgagaccc
     1141 gggtctacgc cgtgctttcc ggcgaggccg tgggcatcga cagccccctg cgtccctggt
     1201 tccagcagag gtatgccgcg ctgcagcggc aagtcgcagg tggcctgcgt cgagcgctcg
     1261 acgggggccg cctccgagcg ggggtcgacg tcgacgccgc cgccgccgag gcgttggccg
     1321 tcatggacgg tctgcagttg cagtatctgc tcgacgacga tgccgacacc tacctggcca
     1381 ggttcgcggc atacatcgac cgcctggtcg tgagcctgag cgcctgaaca cgtcgaccag
     1441 ccggagcgag cgggccatgc cagactggtg gtgaagcgga atcacacccg gttgccgggt
     1501 ggctcggtca ggatggtgtt tgtgccgaac atgacggaag cactgctaga acgacgtctc
     1561 tcgctgatcg ttgagcactt cccgatcgcc gtgctccttg agaccgtgga ccggaaggtg
     1621 tcacagacca accaggcgtt ctgtgacatg ttcggcatcc cggccccgcc cgccgccctc
     1681 gtcggtgccg actgtgaggc tgccgccgtc cagcttgctc cactctgggg cgacctcgat
     1741 ggcttcctgg cccgtgtcca cgaacttctc gctgccggca agcccgtcgt cggggaccgg
     1801 atcgagttga ccgacgggcg agtgctcgaa cgcgacttcc tcatggtccc cgtcgacgag
     1861 actcgcggcg aggccgcgtg gatctatcgc gatgtcaccg tgtcggacac cgctcggcgc
     1921 gtggcgcagt cggaggccaa cgcccgcagt gagctcctgg ccaccatcag ccacgacgtc
     1981 cgcactcccg tcgtcgggat cgtcggcctg gtcgacatcc tcctgcagca accgctggac
     2041 caccggaccc gggagttggt cgagagcgtg cacagctcgg ccgcggccat gaccaccatg
     2101 cttgatgacc tgctggacct ctcccgcgcc gacgccggtc ggctggagct gtgcatcgag
     2161 gacacgagca tctgcgagtt ggtcgaggac gtcgccggga tggtcggtcc ggtcgcccag
     2221 gccaagtccc tgccgctcat cgccggtgtc aaggccacgg tcccggacat catccgcacc
     2281 gatcccgggc ggctgcgcca ggtgctgctc aacctgacct ccaacgccgt gaagttcagc
     2341 ccgaccgggg cggtgacgat cctcgctgac cgggaggggt ccgacctcgt cgtccgggtg
     2401 tccgacaccg ggccgggcat gactcccaag gccatcaccc gcgccttcga gcaatatgtc
     2461 cagggcggcg ctgaggtcaa ccgcgcctac gggggggccg ggctcgggct ggccatcgcc
     2521 aacaagctga cggcggcgtt gggtggcacc atcaccgtcc acagccgcat cgggctgggg
     2581 accaccttcg ccgtccggat ccccggtgcc gtcgtcggcc cggagctgag caccaccatg
     2641 accggcatac gagcgcatct caccgggcac caccgtgccg tccctgtggt ggcggcagca
     2701 ctggaacgcg cgggcatcga ggtgcgagac caggccgacg acgagggggt caacctcgaa
     2761 gtggtcgtcg ccagcagcct ggcgcacgcc cagcggattc cggcggcgag ccggcgtcag
     2821 ctcatccttg tgcccgctgc cctggccagt tgtcccccgc tggctggcac cgcgctcgcc
     2881 ctgccctgga ccagggagcg attgcgcgcc gccctccgag acgaatgggt ggcggacgac
     2941 gtgcgtgagc gtgctgggct gctgcccctc ggaacccggg tcctgctcgc cgaggacgaa
     3001 ccgagcaacc gccggatcat ctccgagatg ctcacccggc ttcgggctga ggtcgttgcc
     3061 gtgggcacgg ggctcgaagc gctggaagcc ctggcggccg accgcttcga cgtcgtcctc
     3121 atggacctga cgatgcccgt catggggggc gtcgaggccg tcgagaacat ccggcatcgg
     3181 gtgccagccg accgatgccc gccgatcctc gcgctcaccg cagatgctcg ggtggacccc
     3241 agcctgcggc cggacagcgg attctgcgga cacctgtcga aacccgtcac cagcgccatg
     3301 ctcagtcgcg ccgtcgccgg ggtgctgaag gcctcctcgc cggagccacc gcccaccttg
     3361 ggcgagcggc ctgcggtcga cgtcaccgtg ctgcatgcgc tcgtcgagga tgtcggtgac
     3421 acccaggtcg tcctcgacac catcgagctc tacctcgaag agcttcccgt gcggttgagc
     3481 tcgatgacgg ctgcgctcac cggcgagcgc ttcgacctgc tgcgcgacga ggcgcatgcg
     3541 ctcaagtcct cgtcacggat gctcggtgcc gggacgttgg ccgaccagtg tcgcgacctt
     3601 gaggcggtca cggccgctca ctcccgacag gacagcgcca gcggcggaac ggtgacccct
     3661 atggtcgacg ccctgcagtt cgaggcgttc cgggtcgcgc aatggttggc ggacttccgg
     3721 gccgcgagtt acccaggcct cgacgtgcct tcggcgagct ccggcacata aagcaggacg
     3781 gtggcgcgca gttcgtcgta agtgaacggc ttggtgaggt gggcatccat cccggcgagg
     3841 aggctgcggt ccgcgtcacc cggggccgag tgagccgtca aggccaccac gggcacgtcg
     3901 cagcgttcgc cccctgcctc gccggcgcgg atcaggcgcg aggcctcgag cccgcccatg
     3961 atcggcatac gaaggtccat cagaacgagg gcgtactcgt gctcccggag cacgtcgagc
     4021 gcttgttgcc cgttctcggc cgtgtcgacc acggcgccca tccgggtgag cattgcggcg
     4081 ctgatctgac gggtcacggc gatgtcgtcc acgagaagga tccgtcggcc gatgccgcgg
     4141 cggtgccggc cctggacggc cacggggatc cgcacctcga tccggcggcg ccggtcgggc
     4201 agggcgtgca cgcagatctc gcccccggcc gcatgcgcca actgacgggc gaggcccagg
     4261 gttgttccct cgctgcccgg gtcgggctca tcgagggtga ggtcggccca ggtggcccgc
     4321 acctcgagtc ggatgcgcac cggtccggcg gactgttcga tcaatcgccc ggccagcagg
     4381 gtgtagatga tgcgacggaa ccgttcgaca tcgacgagca cctcgtccga ctgcgcgaag
     4441 acgacggcca cgtccaagga ggtgtcgcga tggcgcctgg ccgcgtcgtg agcggcgatg
     4501 acgtcgtcga gcactcgtga gaggtccacc acgcgagggt ggtcggcgtt cgcgtcggtg
     4561 ggagcggcga gctcaaccaa tgcgcgaatg cgttgcccga gctcaatgag gtgactcacc
     4621 gcccgagcca ggtgctcgcc ggaatccggt gcggacgccg ccgcctcgat gtcggcgagc
     4681 gcagcgtgta gcggggtctg caggtcgtgg ctgacggtgg agaagagggt tgaacgggca
     4741 tctgactgtg cctccgcgcg gttacgggcc gcgatgaggt cggccgtccg ctgcgccacg
     4801 acatcctgca gggaggtccg gtgtcccgac tcgctctggc gggcgcgcgc gcgctcgacg
     4861 actcggccga gctggatccc gatgacgtcg gcgatctcga gcaggccttg ctggggtcgg
     4921 cgcggactgg agcgcaggaa ctccatgact gccacgacct ccttgccgat gaggatcggg
     4981 aaggcgaagg cggagccgcc agtgagccac tgctgccgag ggaagttacg cgaggcggcg
     5041 atgtcttcct cccaggtagc gccctggagg taggcctcac ccgggatccc cgagcccgga
     5101 gggaagcgca tgccgctcgt tgccgaccgt agctcgtcga ggaacggatc atcgggctcc
     5161 ccactccaga ccccggaact caccatgacg gtcgggtcgt ccttggccgg caggaagtag
     5221 tgggcgacct gccactggct cgcgcgccga atgaggacca tggcgtcgag gaacgccgac
     5281 tcgaactcct gcgcgctgtt ggcgatggac gtcacctggc tgagcagatc gacgtcggcc
     5341 tggcgcaggt aaagccggcg aagggtggcc tcggcgatgt cctcggactc gcgccgggcg
     5401 gcccgttcgc gcttcagccg ggcttcggcc cgggccagtt gtcggcgaag ctcggcatcg
     5461 gcgggatgtt gttcactcac cgccgggcct cgggccagat gaactgcagc tcacaatggt
     5521 cgccgccgcg gtgctgacag atcggctggg tcacgaccat cgtctccccg aagtggtcag
     5581 ccgcaccgag agcgaggccc tcaccgagat agcacatgcc ccggtttgag cggtacgaca
     5641 ggctcatccg gtgcccctga ccggagacga cgtcgaacac cgggacatcc gctccgggat
     5701 agaccttgcg gacctcggga tgaatgacgg agttgaggcc gagcagcagc gaccgtgcgt
     5761 ccttaaagtc gatcatgagg tccgcattac gcgcggccag ccgactgaac cccactcggc
     5821 cgaggtgccg cagcagctcc tcctggctca ggccgagtgc cgcgctcgcc gtctggacca
     5881 gcaccgccat ctcgtggtcg gggtaggtcc ccaaggaggt gtaggcgccg tccagcccgg
     5941 cggtgtccag aagttcatcc caggcatcct cgccatgttc ggcgcgcacc gtctcctcga
     6001 tgacgttgaa gatgacaccc ttcattgcca caccctctca ttcctcgagc cgtggcggcg
     6061 gccgtcgaaa cactccacgc cggccgaggt ctgccggctg cgtcaggccc gcgtctcctt
     6121 aggtttgtgg acaaaactca aggactgctc aaggggttct caggacatcc ccacgcatgg
     6181 gcctcgggcc cttgacggcc cttaccgtgg agttatgccc acccccacgc tgccggtcac
     6241 ggctgctgaa cttctggctc ggcccccctt gcggggtcgt ttgccggtgc gaggccgggt
     6301 aagggaggcc cgggaggcct gggggggcgc cgccatcggc gagcgcctgg ccgcaatgga
     6361 cgagcggcgc gagtgcctgg gggcgcccct cgtccgagac gtcgcacggg tcctggaagg
     6421 cgcggaccgt acgaccgcgc cggtgggcgg gacccggcga cgtctgcttc tcgccaccgt
     6481 cgctcccgcc gccgttgcgc gagcggctcg atagcgccgg gttcgctacg cgcgcagcct
     6541 ttttcggcat ggcgcatcgc aacctacccg gtgtcgcctt agcccgccgg gtgctgcgcg
     6601 tcgggggcgt ggagtcggtc acagccacgc ctgtcgcagc acccggtgcc gcggtggtca
     6661 tcgaccacgc ttccggtatg ccgctcgcgg cctccccgac acgcggaagg cggtctcgct
     6721 gatcgttcag cccatcgccg tcttcgccga gaactcgccg aagacgtcga cgatgcgggt
     6781 catacgcaca gcccggccgg cccacggctg acccgccgcg ggctgaccag gcctgcccgc
     6841 tgcgccttgc tcatgcccgc caccaggccc gcagtctcca cggatttgag gataaccagg
     6901 ccgcccgtgc gcaccggcga ggccagcagg gcgggaagtt gcgagcggag gccggggcgg
     6961 ctcacgtgct ggcgagtctc gggcttccgg ctgaggtact cggccaccca tcgaccgtcg
     7021 tatcgcttct tagcgaactg gtctcgtagc gagatcttgc cctcgtcgtg ccagatccag
     7081 gaggagaccc gcgcgatctc gacgccggcg gctcgggtgc ggtccgcgag gtcccagtcc
     7141 tcggccgccg acagcgtctc gtcccagcgg ccgacgaggt cgaacacctc ggtgcggaac
     7201 gctcgcggag cctcgacatc gtccaacccg acgtgcaggc tcttctccag gacccggcaa
     7261 gaggccagga agcccacacc gaaggaacgc tgcgggatga tgactgcacc cacctcggga
     7321 cgggcttcga aggtcgcggc gaggtcggcc aggacggtcg gttcgaggac catgtcggag
     7381 tcgatgaaca cgacgatctc accgctggcg agccaggcac cccggttgcg ttgcgcgctg
     7441 cgctccggcc cgcggtcgac gatgatgtga gccagctgca gggcgattgg tggggtgtcg
     7501 tcggtcgagg cgttgtcgac cacgatgatc tcgacgtcct cgtgggtctg tctgcggagg
     7561 gatcccagac atgcggcgag cgtcctcccg gagttgcggg tggggacgat gacgctgatc
     7621 ttcatgagat ctcctcaggg atgtcgccgt aggcgatcgg gtcgcagttg cgacgttcat
     7681 cgcggccgcg gggagccggc agcagcacgg cataccggag ggtgcacacc aggacggcag
     7741 cgagcgcagc gaacgcgctc ggcgccaacg ccatcggtgc gatggcgagg gcgggtgaga
     7801 gcgtcaacgt ggtcacttga cgaatgtcgc aacgtcagat caagcgacgc tcaagcccgt
     7861 cgcaacgttg cagcaggctt ggttgggcgg gctcggtggg cggcggggaa gcggaggtcg
     7921 gccaacgact ggaccgagat tgccaccacg accggcgcgg cgagcgccgc caagcgaccg
     7981 gtcgtttggg gccgcgcacc gctcccggct cgggggcgcc agggagcggt gtgcggcatg
     8041 aacatcagcg tgcccggctg atctcaagaa ggactcaaga gaggttccgg gcttgccggt
     8101 catccgaccg cggagtgacc ttggcgacgt gctcccgcgc cggatcgatc ccgtgaacga
     8161 ccacggagga gtcgttcgag atggtgtcga ccaagacgcg gatgatcctc gactccgagt
     8221 cgcagacgac cattccggag gcacctcaag ggaggttcaa gcgcgaggca acgcgcggtt
     8281 cggtccttcg ccttttcggc gggcttagcg tttgctgttc ggctcgaagc ggaacccgac
     8341 gccgcgcacc gtgtgcacga agcgtggtct gctccctgat tcgccgagct tcttgcgcag
     8401 gttgccgacg tggacgtcca cgacgtgatc gtcgctgaac cagtcgacgc cccacaccgc
     8461 gtcgagcagc tgtgagcggg tgaaggcccg acgcggctcg gaggccagct tggccaggag
     8521 gtcgaactcg accttcgtca ggtcgaggag ctcgccgtcg agggtggtct cgcgagcggc
     8581 gacgtcgatg cgcagcgggc cgaagtccca gagctccggg tcggggacga cccgggggcg
     8641 gcggcgcatg gcggcgatcc gcgccgccag ctcgcgcgcc gagaacggct tggtgacgta
     8701 atcgtcggcg cccatcgtca ggccgatgac ccggtccacc tcgtcggtgc gcccggtgac
     8761 catgatgagg taggcatcgg agaagtcgcg taggcggcgg catacctcaa agccgtcgac
     8821 tccgggcaga ccgatgtcga ggagcacgag ctcgggtttg cgcgcgcggg ccaggcccag
     8881 ccccgtctca ccgtcgtgag cgaccaggac gttgaaaccc tcgcgctgca ggagcttggc
     8941 gccgatgagc gcgtactcct tgctgtcctc aatgaggagc gcaaccggtg cgaccatgcg
     9001 ggacattgtc gcaggagcgc ccccgctcgg cccgttggtt cgtgaggtgc ggcgtgtccg
     9061 aattgtccgg gggtggcggg ggtgtggcgg ggtggtcccg cacaggggat accaatggtc
     9121 cgatgtgggc ggcatacccg tgcgtggcag gggccgcggt cggtcaccat tggcatcccg
     9181 tatgccgcgc gctgacctgg cattcgcaca gttcggcaca ctgagtgaca cggtgaccac
     9241 cggcgctgcg agtagagtca ctcc
//
LOCUS       DAOMZY010000118         9247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_7056_length_9247_cov_6.502502, whole genome shotgun sequence.
ACCESSION   DAOMZY010000118 DAOMZY010000000
VERSION     DAOMZY010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9247
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7056_length_9247_cov_6.502502"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PLK64_08105"
     CDS             <1..517
                     /locus_tag="PLK64_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPV79747.1"
                     /translation="VAGPYFDISAPEPTGVVAVLAPQASSLLGLVSVLAPVIVSGNTA
                     VLVASQDRPLPAVTLTECLATSDVPGGVVNVITGRTAEIAPWLASHRDVNAIDLAGAT
                     DGSNAAATADGKAVTWADLERAAADNLKRVLRPAGSGAAAVEPDWAPTPDLSRMTAFL
                     ETKTVWHPKGR"
     gene            553..1242
                     /locus_tag="PLK64_08110"
     CDS             553..1242
                     /locus_tag="PLK64_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPV79748.1"
                     /translation="MGQSARRARVVVLTGPSGAGKSRLAARLHAAYGWPIVRLDDFYR
                     DGDDPDLPMVTLGGHDLPDWDDPRSWNAPAAIAALEELLDTGTTRVPTYDIATSRAIG
                     HSQATAVATDLVLAEGIFAAEIIPALVERGRLAGAFCITHGRTPNAVLRFVRDLREGR
                     KPPLVLLRRGIELWRREPRIVSRAASLGASCGSPASVLKEIRTRLIASGETTRPSDRG
                     PEPWEHVRRPG"
     gene            1247..2311
                     /locus_tag="PLK64_08115"
     CDS             1247..2311
                     /locus_tag="PLK64_08115"
                     /inference="COORDINATES: protein motif:HMM:NF021615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HPV79749.1"
                     /translation="MSGFGPPPGPPEAPVHVRRASYLPPGGLGARPAASVAANRGRYA
                     LTRGAKPGIIPLAPLRLGDLLDGSVKHIRRNPGPVLGVSALANAFSVLPVLVLVTAAF
                     AGSWLRATRVSTIMDAWAVGAALNLVGTIYATLVLTAVLSYAAAEAALGRRRRLGEIW
                     AAVRSRIWTVLGSQALVVLAAALPWALLVGMLSIAGQDSVWVVLLVGLGFGLLAIAAN
                     AFVLPRLLYSGPAAVLEGLSLRRSIARSWSLSRGRAWSLVGVLCVVVGLAFIVYWVVE
                     LPQLGIYGLLTGFLEITGPAADSIGNFNFALANLGAAILVTPFVATSMALHYLDARIR
                     KEGFDLALQRAATADMEARS"
     gene            2308..3021
                     /locus_tag="PLK64_08120"
     CDS             2308..3021
                     /locus_tag="PLK64_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HPV79750.1"
                     /translation="MSVLATASAWLTGLAQDLNPDGSEARQWLADELANDEYRDGRPL
                     LTRIIEWLVDVITDLLSGFRVTGVSGGGAPPIVVALVVVVILAGLALLLSRLRRERRT
                     VTSSDAVLGDLNLSVVEFRDRGRASIREGRWNDAVIDFTRAIAREAADRTLLSDAPSL
                     TAHEIGAQLAPVFPEHAEATARTMDLFDSVRYGRYAATEADARATQAHDETLRKARPV
                     LAGHATLPVADDNIVTRPS"
     gene            3018..4184
                     /locus_tag="PLK64_08125"
     CDS             3018..4184
                     /locus_tag="PLK64_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HPV79751.1"
                     /translation="MTTTLWRTDAPTEGPPGRRWWRIAVLVAVVIGAAYLLGRGGQPG
                     SDAAYHPENPSAKGAQAVARVLAAHGVTVIVAEGQAALQRAPIDGDTTIVVSKSSDLR
                     EVTLQALATAARPAERLVLIHPERRVVRALTPGVSMAEVAHSQNALVSTCDTPDVHPG
                     ERLSRSQSQYRDTKATASCYVNDGQAVYLVTSGPGLREVVLIGSTDPITNDRTAEVEN
                     GALALRALGHSSRLVWYVPDLRDVPPTAAEQTESFTPVWWGPMLGLLAFTMLAVFWWR
                     GRRFGRLVTEPLPVVVRAVETTESRGRMYHKARDSERAGAVLRDASGRRLTAYLGLAP
                     GTPADLVAQAVSAATGQPLDQTRWLLGGPPVTTDTDLLDLAARLAALEKEIRRS"
     gene            4181..5221
                     /locus_tag="PLK64_08130"
     CDS             4181..5221
                     /locus_tag="PLK64_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPV79752.1"
                     /translation="MTEQPWSATPPAAPAAGPSNPPAPTSPAASPGGARAALLAVRGE
                     VGKAVVGQDAAVTGILIALLSRGHVLLEGVPGVAKTLLVRAVAQALSVETTRVQFTPD
                     LMPGDITGSLIYDNATSEFSFREGPVFTNLLLADEINRTPPKTQASLLEAMEERQVSV
                     DGHPRKLPVPFLVAATQNPVEYEGTYPLPEAQLDRFLLKVVLPVPPREQEIEIVTRHA
                     AGFDPRDLVAAGIRAVASAADLAAGAAEVRQVRIAPEVAAYIVDIGRATRQSPSLSLG
                     VSPRGATALLATSRAWAWMNGRTFVTPDDVKALAHATLAHRLTLRPEAELEGVGVAAV
                     LATAIASVPVPR"
     gene            5241..6536
                     /locus_tag="PLK64_08135"
     CDS             5241..6536
                     /locus_tag="PLK64_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011775358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPV79753.1"
                     /translation="MTVSGRLPILVVLGAVAVVLEPTRRTVLLWALVCLALVLLDVLL
                     ALSPTRLVIEREPVPSVRLGESAQSGVLVTNPTRRTLHGHLRDAWVPSAGARGERHTA
                     RIKGGERRRFTTTLHPTRRGDRHADRVTVRSFGPLRLAARQRSFDVAGTIRALPPFES
                     RKHLPSRLASLRQLDGRSAVRTRGQGTEFDSLRDYVEGDDVRSIDWRATARRRNVVVR
                     TWQPERDRRVIIVLDTSRTSAGRVGDQPRLDAAMDAALLLAALASRAGDRVDFLAGDR
                     WLRARVAAAANRTTLLNDLVQAMAPLDPALLEASWPTLASAIADTSRRRALVVLLTPL
                     EPAAIQESLLPSLPALTRHHRVVIASVADPAIAALAETRETTKDAYAAAAAERTMALQ
                     DRTAAVLRRMGVAVIDASPAELPPKLADHYLMLKAQGLL"
     assembly_gap    6591..6690
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6761..7759)
                     /locus_tag="PLK64_08140"
     CDS             complement(6761..7759)
                     /locus_tag="PLK64_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HPV79754.1"
                     /translation="MDLDAYVAAHTHEWSRLEQLVGQRGKLQGAQADELLDLYQRTAT
                     HLSEIRSTAPEPSVVAYLSSMLARARSRAVGTRSTSWSDVGRFFVVTFPAALYRTRRW
                     WLTTMAINVVVGFAIGWWFVINPQFESALASPAEIQQLVTHDFADYYSEYAAGSFALK
                     VWTNNAWVTALCISFGVLGLPVLWLLWNNILNVSVIGAIMWRYDRADLFFGLILPHGL
                     LELTAVFVAAGVGLRMFWAWIEPGPQTRMTAIAREGRAAMSVALGLVVVLFISGVIEA
                     FVTPSPLPTWARIGIGILAEAVFLTYVFTLGRYAVRRGETGDVAAVDQGDTAPVVG"
     gene            7778..8626
                     /locus_tag="PLK64_08145"
     CDS             7778..8626
                     /locus_tag="PLK64_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPV79755.1"
                     /translation="MSQPMIATIGVTEDDLVTGEAVALDLPPASVGLRILAGVLDLII
                     VQVLTYVVFVAGALLSNQLDGALGASILVLLTVACLVGYPTAMLTLTRGKTVGKLAAG
                     LRVVRDDAGPITFRHALTRSLVGVIEVFAFYGVPAVIASIINPRGKRLGDMAAGTYVV
                     RERTRASLPPPVPMPPHLAVWAASADIAQLPDRLAADVRTFLPAAFGMDPNAREELGR
                     ALLAEVMEFVSPLPPPGNHPEYILAAVVADRRRRDLGRLMRDATLRDRLIPPDPLVTP
                     SSGMPR"
     gene            complement(8684..>9247)
                     /locus_tag="PLK64_08150"
     CDS             complement(8684..>9247)
                     /locus_tag="PLK64_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HPV79756.1"
                     /translation="HFTIHAGEAFGLPSIWEAIQYCSADRLGHGVRIVDDITIDGVPV
                     PLWVAQAHAAHVNTYDEVDLSTVRLGRLAAYIRDSRIPLEMCPSSNLQTGAARSIGLH
                     PISLLLRLRFRVTLNTDNRLMSGTSMSREAALLVEHAGWTLGDLRWVTINAMKSAFLP
                     FDERLAFIDDTIKPGYAALGASASAPR"
BASE COUNT         1285 a         3435 c         3060 g         1367 t
ORIGIN      
        1 ggtcgccgga ccctacttcg acatctcggc ccccgagccg accggcgtcg tcgccgtgct
       61 ggccccgcag gcgtcctcgc tgctcgggct cgtgtcggtg ctcgccccgg tgatcgtctc
      121 gggcaacacc gcggtgctcg tcgccagcca ggaccggccg ctgcccgccg tgaccctcac
      181 cgagtgcctc gcgacctccg acgtgcccgg cggcgtcgtc aacgtcatca ccggtcgcac
      241 cgccgagatc gcgccctggc tggcctcgca ccgggacgtc aacgccatcg acctggccgg
      301 cgccactgac ggctccaacg cagccgccac cgctgacggc aaggcagtga cctgggccga
      361 tctggagcgc gcagccgcag acaacctcaa gcgggtcctg cgaccggccg ggtccggcgc
      421 agcagcggtc gaacccgact gggcaccgac gccagatctg tcccggatga ccgcgttcct
      481 cgagaccaag accgtctggc accccaaggg ccgctaggcc gccgggtcgc ccgaagctag
      541 gctctgtgct ccgtgggaca gtctgcgcgt cgggcccggg tcgttgtgct caccggaccc
      601 tcgggggccg ggaagtcccg gctggcggcc aggctgcacg cggcatacgg ctggccgatc
      661 gtccgcctgg atgacttcta tcgcgacggc gacgacccag acttgccgat ggtcaccctc
      721 ggcgggcacg acctgccgga ctgggacgac ccgcgatcct ggaacgcccc ggcggccatc
      781 gccgcgctcg aggaactcct cgacaccggc acgacccgcg tcccgaccta cgacatcgcg
      841 acgtcccgcg ccatcggcca cagccaggcc accgcggtgg ccaccgacct cgtgctcgcc
      901 gaggggatct tcgccgccga gatcatcccg gcgttggtgg aacgcggccg cctcgcgggc
      961 gccttctgca ttacccacgg ccggaccccc aacgccgtgt tgcgcttcgt ccgcgacctg
     1021 cgggaggggc gcaagccgcc gctcgtcctg ctccgacgcg ggatcgagtt gtggcggcgg
     1081 gaaccgcgga tcgtctcgcg tgccgcgagt ctcggcgcgt cgtgcgggtc ccctgcctcg
     1141 gtgctcaaag agatccggac ccgcctgata gcgtcgggtg aaaccacacg accgtcggac
     1201 aggggaccag agccgtggga gcacgttcgt cgcccagggt gacgtggtga gcggcttcgg
     1261 cccgccaccc gggccacccg aggcgccggt ccacgtgcga cgggccagtt atctgccccc
     1321 gggcggcctg ggtgctcgcc cggcagcgag cgtcgcggcg aatcgcggtc gctatgccct
     1381 cacccgcggc gccaagccgg ggatcatccc cttggccccg ttgcgcttgg gcgacctgct
     1441 cgacgggtcg gtcaaacaca tccggcgcaa ccccggcccc gtgctcggcg tctccgccct
     1501 ggccaacgcc ttcagcgtcc tgccggtcct cgtcctggtc accgcggcct tcgcgggctc
     1561 gtggctgcgc gcgacccgag tgtcgacgat catggacgcg tgggccgtgg gggcggcgct
     1621 caacctcgtg ggcacgatct acgccacgct cgtgctcacc gcagtgctct cgtatgccgc
     1681 cgccgaggcc gccttgggtc gacgccgtcg cctcggcgag atctgggccg ccgtccgctc
     1741 ccggatctgg acggtgctcg gaagccaggc gctcgtcgtc ctggcggccg ccctgccctg
     1801 ggctctgctc gtcgggatgc tctcgatcgc cgggcaagac tcggtctggg tcgtcctgct
     1861 cgtcgggctc gggttcgggt tgctcgccat cgcggccaac gccttcgtcc tgccacgcct
     1921 gctctacagc ggcccggccg ccgtcctgga agggctgtcc ctacgacgtt ccattgcccg
     1981 ctcgtggtcc ttgtcccgcg ggcgcgcctg gtccctcgtc ggcgtcctct gcgtcgtcgt
     2041 cgggctggcc ttcatcgtct actgggtggt ggagttgccc cagttgggca tctacggcct
     2101 gctgaccggc ttcctcgaga tcaccggtcc ggcggccgac tcgatcggta atttcaactt
     2161 tgccttggcc aacctgggcg ccgcgatcct cgtcacgccg ttcgtcgcca cctcgatggc
     2221 gctgcattat ctggacgccc ggattcgtaa agaaggcttc gacctcgcgc tgcagcgcgc
     2281 cgccacagcc gacatggagg ctcggtcgtg agcgtccttg ccaccgcctc agcctggctc
     2341 accggtctgg cccaggacct caatcccgac ggttccgaag cccggcagtg gctggccgac
     2401 gagttggcca acgacgaata tcgcgacggg cgtccgttgc tgacgcgcat catcgagtgg
     2461 ctcgtcgatg tcatcaccga cctgttgtcc ggcttccggg tcaccggtgt ctccggtggc
     2521 ggggccccgc ccatcgtcgt cgccctcgtg gtcgtggtga tcctcgccgg cctggccctc
     2581 ttgctcagca gactccgccg cgaacgacgc acggtgacca gcagtgacgc ggtcctcggc
     2641 gacctcaacc tcagcgtggt cgagttccgc gaccgcggcc gcgcctcgat ccgcgaaggc
     2701 cggtggaacg acgcggtcat cgacttcacc agagccatcg cccgcgaggc cgccgaccgc
     2761 accctgctgt ccgacgcccc ctcgctcacc gcccacgaga tcggcgccca actcgccccc
     2821 gtcttccccg agcacgcaga ggcgaccgcc cgcacgatgg acctgttcga ctcggtccgt
     2881 tacggccggt atgccgcgac cgaggccgac gcccgggcaa cgcaggcgca tgacgagacc
     2941 ctgcgcaagg cccgtccggt cctggccggg cacgccaccc tccccgtcgc ggacgacaac
     3001 atcgtgacga ggccatcgtg acaacgacct tgtggcgcac cgacgctccg accgaaggcc
     3061 cgccgggccg acgctggtgg cgaatcgccg tcctggtcgc ggtcgtcatc ggggcggcat
     3121 acctgctcgg ccggggcggg cagcccggca gcgacgcggc ataccacccg gagaacccgt
     3181 cggcgaaagg cgcccaagcg gtcgcccggg tcttggccgc acacggcgtc acggtgatcg
     3241 tcgccgaggg ccaggccgcg cttcagcgtg cgccgatcga cggtgacacc accatcgtcg
     3301 tcagcaagtc gtccgacctg cgcgaggtca cgttgcaggc gctagcgacc gcggcccgcc
     3361 cggccgagcg cctcgtgttg atccatcccg aacgccgggt ggtgcgcgcc ctcactcctg
     3421 gcgtgagcat ggcggaggtc gcccactcac agaacgcgct ggtcagcacg tgcgacaccc
     3481 ccgacgtgca ccccggtgag cggctgagcc gctcgcaatc gcaatatcgc gacaccaagg
     3541 cgaccgcgag ttgctacgtc aacgacgggc aggccgtcta tctcgtcacg tccggccccg
     3601 ggctgcgcga ggtggtcctc atcgggagca ccgacccgat caccaacgat cgcaccgctg
     3661 aggtcgaaaa cggagcgctg gccctgcggg cgttgggcca ctcctcccgc ctggtctggt
     3721 acgtccccga cctgcgcgac gtgcctccga cagcggctga gcagaccgag tccttcacgc
     3781 ccgtgtggtg gggccccatg ctggggctgc tggcgttcac catgctggcc gtgttctggt
     3841 ggcgcggacg acggttcggg cgcctggtca ccgaacccct gccggtcgtc gtccgcgcgg
     3901 tcgagaccac cgagagccgc ggccggatgt atcacaaggc tcgcgacagc gaacgcgccg
     3961 gagcggtcct gcgcgatgcc agcggacggc ggctcacggc atacctcggc ctggcgcccg
     4021 gcacccccgc cgacctggtc gcccaagccg tgtccgcggc aaccggccaa cccctcgatc
     4081 agacccgatg gctcctgggt gggccgccgg tcaccaccga caccgacctg ttggacctcg
     4141 ccgcgcgact ggcggccctg gagaaggaga tccgtcgctc atgactgagc agccctggag
     4201 cgccacgccg cccgccgcgc ccgccgccgg cccctcgaac ccgccagccc ccacgtcgcc
     4261 tgccgcgtcg cccggtggcg cccgtgcggc gcttctcgcg gtgcgcggcg aggtcggcaa
     4321 agcggtcgtc ggtcaggacg ccgccgtgac gggcatcctc atcgccctgc tgtcccgggg
     4381 ccacgtgttg ctcgaaggcg tccctggcgt ggccaagacc ctgctcgtgc gcgcggtcgc
     4441 ccaagcgctg tcggtggaga ccacgcgggt gcagttcacg cccgacctca tgcccggcga
     4501 tatcaccggg tcgttgatct acgacaacgc caccagcgag ttctccttcc gcgagggtcc
     4561 ggtcttcacc aacctcctgc tcgcggacga gatcaaccga acgcccccca agacccaggc
     4621 atcgctcctg gaagcgatgg aggagcggca ggtgtccgtc gacgggcacc cccgcaaact
     4681 gccggtgccc ttcctcgtcg ccgcgaccca gaaccccgtg gagtacgaag gcacctaccc
     4741 gctgcccgag gcccagttgg accggttcct gctcaaggtg gtccttccgg tgccgccgcg
     4801 agagcaggag atcgagatcg tcacccgcca tgccgccggg tttgacccgc gtgacctcgt
     4861 tgctgcgggg atccgcgcgg tggcgtcggc cgccgatctc gccgcagggg ccgccgaggt
     4921 gcgtcaggtc cggatcgcgc cggaggtcgc ggcatacatc gtcgacatcg gccgcgcgac
     4981 ccgccagtcg ccctcgctgt cgctcggagt gtcgccgcgt ggcgcgaccg cgctgctggc
     5041 tacgtcccgg gcctgggcct ggatgaacgg gcgcaccttc gtcacccccg acgacgtcaa
     5101 ggcgctcgcc cacgccaccc tcgcccaccg gctcaccctg cgcccggagg ccgagctcga
     5161 aggcgtcggc gtcgcggcgg tcctcgcgac ggcgatcgcc tcggtcccgg tcccccgctg
     5221 accggcgcga agggccaacc atgaccgtca gcggacgact accgatcctc gtagtgctcg
     5281 gggcggtcgc cgtcgtgctg gagccgactc ggcgcaccgt gctcctctgg gcgctcgtct
     5341 gcctcgcgct cgtgcttctg gacgtcctgc tggccctgtc accgacccgg ttggtcatcg
     5401 aacgcgaacc tgttccctcg gtgcggctcg gcgagtcggc ccagtctggg gtcctcgtca
     5461 ccaaccccac ccgacgcacc ctgcatgggc acctgcgcga tgcttgggtg ccttcggccg
     5521 gggcccgggg cgagcggcat actgcgcgga tcaagggggg cgagcgccgc cggttcacga
     5581 cgacgctgca cccgacccgc cggggcgacc ggcacgccga ccgggtgacg gtccgctcgt
     5641 tcgggccgct gcggctggcc gccaggcagc gatcgttcga cgtcgccggg acgatccggg
     5701 cgttgccccc gttcgagtcg cgcaaacacc tgccctcccg cctggcctcg ttgcgccagt
     5761 tggatgggcg ctcggcggtt cggacccgag gacaggggac ggagttcgac tcactgcgcg
     5821 actacgtcga gggcgacgac gtgcgctcga tcgactggcg cgccacagcg cggcggcgca
     5881 atgtcgtcgt gcgcacctgg caacccgagc gcgaccggcg ggtcatcatc gtcttggaca
     5941 cctcccggac ctcggccggc cgggtcggcg accagccccg tctggacgcc gcgatggatg
     6001 ccgcgctgct gctcgccgcg ctggcttccc gcgccggcga ccgggtcgac ttcctcgccg
     6061 gcgaccgctg gctgcgggcc cgcgtcgcag cggccgccaa ccgcacgacc ctgctcaacg
     6121 acctcgttca ggcgatggcg ccgctggatc ccgctctcct ggaagcctcg tggcccaccc
     6181 tggccagcgc catcgccgac accagtcgtc gccgagccct cgtcgtgctc ctcacccccc
     6241 tcgagccggc ggcgatccag gagtcactgc tgcccagcct cccggccctc acgcgccacc
     6301 atcgggtcgt catcgcgtcg gtcgccgacc cggccatcgc cgcgctcgcc gagacccgag
     6361 agaccaccaa ggacgcgtat gccgcggctg cagccgaacg caccatggcg ctgcaggacc
     6421 gcaccgcagc cgtgctacgc aggatgggcg tggctgtcat cgacgccagc ccggccgagt
     6481 tgccgcccaa actcgccgac cactacctca tgctcaaggc gcagggcctg ctctagacag
     6541 tcccgatcgc gacagtcccg atcgcgacgg tccccggtcg cgctcagggc nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gacagtcccg atcgcgacag tcccgatcgc
     6721 gacggtcccc ggtcgcgctc agggcgagtg agcccggatc tcagccgacg acgggtgctg
     6781 tgtcgccctg gtccactgcg gcgacgtcgc ccgtctcacc gcggcggacg gcataccggc
     6841 cgagggtgaa gacgtaggtg aggaacaccg cctccgcgag gatgccgatg ccgatccggg
     6901 cccaggtcgg caacggcgag ggggtgacga aggcctcgat gacgccggag atgaacaaca
     6961 cgacgaccaa gccgagcgcg acggacatgg ccgctcgacc ttcgcgggcg atggccgtca
     7021 tccgggtctg cggccccggc tcgatccagg cccagaacat gcgcagcccg acgcccgccg
     7081 cgacgaacac cgcggtgagc tcgagcaggc cgtgcggcag gatcaggccg aagaacaggt
     7141 cagcccggtc gtagcgccac atgatcgcgc cgatgacaga gacgttgagg atgttgttcc
     7201 acagcagcca gaggaccggc agccccaaga cgccgaacga gatgcacagg gcggtgaccc
     7261 aggcgttgtt tgtccacacc ttcaatgcga aggagccggc ggcatactcg ctgtagtagt
     7321 cggcgaagtc atgggtgacg agttgctgga tctccgccgg ggacgcgagg gctgactcga
     7381 actgcgggtt gatgacgaac caccacccga tggcgaagcc gacgacgacg ttgatcgcca
     7441 tcgtcgtcag ccaccagcgg cgagtgcgat agagggccgc cgggaaggtc acgacgaaga
     7501 atcgcccgac gtcgctccac gacgtcgagc gggtcccgac ggctcgggac cgggcgcggg
     7561 cgagcatcga ggacaggtag gcgacgacgg aaggctccgg cgcggtggag cggatctcgg
     7621 acaggtgggt ggccgtgcgc tggtagaggt cgagcaattc gtcggcctgt gcgccctgga
     7681 gtttgcctcg ttgaccgacc agttgctcga gccgcgacca ctcatgagtg tgcgcggcca
     7741 cgtaggcatc caggtccaca cggttactgt atgccgtgtg agccagccaa tgatcgcgac
     7801 gatcggtgtc accgaggacg acctcgtcac cggtgaggcg gtcgccctcg acctgccccc
     7861 ggcctcggtc gggctgcgga tcctcgccgg cgtgctcgac ctgatcatcg tccaagtcct
     7921 gacctatgtc gtcttcgtcg ccggcgccct gttgtccaac caactcgacg gtgccctcgg
     7981 tgcttcgatc ctcgtgctgc tcacggtggc ctgcctcgtc ggctacccga cggccatgct
     8041 caccctgacg cggggcaaga cggtgggcaa actggccgcc ggcctgcggg tggtgcgtga
     8101 cgacgcggga ccgatcacct tccgtcacgc gttaacccgc agcctcgtcg gcgtcatcga
     8161 ggtgttcgcc ttctacgggg tccccgccgt catcgcgtcc atcatcaacc cgcgcggcaa
     8221 gcgcttgggt gacatggctg ccggcaccta cgtcgtgcgt gagcgcaccc gggcgagcct
     8281 tccgccgccg gtccccatgc cgccgcacct ggccgtgtgg gccgcgtcgg ccgacatcgc
     8341 ccaactgccc gaccggctcg ccgcagacgt gcggaccttc cttccggcgg cgttcgggat
     8401 ggacccgaac gctcgggagg agttgggccg cgcgctgctc gccgaggtca tggagttcgt
     8461 ctcgcccctg ccgccgccag gcaaccaccc ggaatacatc ctcgcggccg tggtcgccga
     8521 ccgccgccgc cgcgacctcg gccggctgat gcgcgacgcc accctgcgcg accgactcat
     8581 cccgccggac ccgctggtca ccccgtcatc aggtatgccg cgctgacccg ttcgacccgc
     8641 gggccggccc caaccgaggg ccgctcctac cgaggggccg acgctaccga ggggccgacg
     8701 cagaggcccc gagggcggca taccccggct tgatcgtgtc gtcgatgaac gcgagccgct
     8761 cgtcgaacgg caggaacgcc gacttcatcg cgttgatcgt cacccagcgg agatcgccca
     8821 acgtccaccc ggcgtgctcg acgagcagcg cagcctcgcg tgacatcgag gtgccgctca
     8881 tcaaccggtt gtcggtgttg agcgtgaccc ggaaccgcag gcgcaacaac agggagatcg
     8941 ggtgcaggcc gatgctccgc gcggcgccgg tctgcaggtt gctgctcggg cacatctcca
     9001 gggggatccg ggagtcgcgg atgtatgccg cgagcctccc gagccgcacc gtcgacaggt
     9061 ccacctcgtc gtaggtgttg acgtgcgcgg cgtgcgcctg agccacccag aggggcacgg
     9121 gcaccccgtc gatggtgatg tcgtcgacga tccgcacccc gtggccgagc cgatcagccg
     9181 agcagtactg gatggcctcc cagatgctcg gcaatccgaa ggcttcgccc gcgtggatgg
     9241 tgaagtg
//
LOCUS       DAOMZY010000119         9208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_7108_length_9208_cov_6.900688, whole genome shotgun sequence.
ACCESSION   DAOMZY010000119 DAOMZY010000000
VERSION     DAOMZY010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9208
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7108_length_9208_cov_6.900688"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PLK64_08155"
     CDS             <1..554
                     /locus_tag="PLK64_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018775675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HPV79757.1"
                     /translation="DEDDADGGDDEASGPSAGRSRGSRSRSRVALLEREGEVAADFLE
                     RLLDIADLDGDIEVDIDGDRAAVSIVDSEEGRVPRRLVGPDGKVLDALQELTRLAVQT
                     ATGERSRLMLDVAGFRADRRTALVTLAQEAIAEVRASGVARALDNMSAFERKVVHDEV
                     TAAGLVSESEGVEPHRHIVISPA"
     gene            551..1324
                     /gene="rsmG"
                     /locus_tag="PLK64_08160"
     CDS             551..1324
                     /gene="rsmG"
                     /locus_tag="PLK64_08160"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPV79758.1"
                     /translation="MTEVDVPVPPLSAAQVFGPREELARAYVGLLADTGISHGLIGPR
                     EVPRLWDRHVLNCAVIQELLPPNALCADIGSGAGLPGLALAIARPDITMHLVEPLQRR
                     ARWLDVAVAELGLSGVTVHCSRADALHGVLTVDVVTARAVAELATLGQWCLPLLPPGG
                     RLLAIKGSKAEAELAEAWPALQRLGCQAGSVRECGGSMIDPPTRVVEVIAGSAPSSGK
                     GRARPPSARAQQRKARAAAARRHRGGFSPTGADEASPSN"
     gene            1772..2671
                     /locus_tag="PLK64_08165"
     CDS             1772..2671
                     /locus_tag="PLK64_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HPV79759.1"
                     /translation="MDLQDESTPLAAAIAQDVRRRSTLVGAPLPRPPATRVLTVANQK
                     GGVGKTTSAVNVAAALAQSGMRVLVIDLDPQGNASTALGVDHRAGIDSIYDVLVDGQP
                     MSAVVVESPTVPGLFCAPSTIDLAGAEIELVSLVAREGRLATALNAHLADTAYDYVFI
                     DCPPSLGLLTVNAFVAAREVFIPIQCEYYALEGLSQLLRNVELIRTHLNPTLAVSTIL
                     LTMFDGRTRLSAQVAEEVREHFPEQTLRTTIPRSVRVSEAPSFGETVMTYDPLSSGAL
                     AYLEAASQIAQRGAESMNEGAHP"
     gene            2668..3810
                     /locus_tag="PLK64_08170"
     CDS             2668..3810
                     /locus_tag="PLK64_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPV79760.1"
                     /translation="MKEREKRRALGRGLGALIPTAPAGERPVDVFFGPAPSAITVDIG
                     TKHAAADGDPSDLTDSAETTDPTDAAAASSSSSPTDAPSAGTGPADPDNDALRPVPGA
                     TFAELPVESIRPNPRQPRTVFDEDDMAELVHSVSEIGILQPIVVRRVDDGGPLGSGHD
                     AAIATYELVMGERRWRAAQAAGLEHVPAIIRSTHDDDLLRDALLENLHRSQLNPLEEA
                     AAYRQLLDDFGCTQEELATRIGRSRPQVSNTLRLLKLPPLVQRRLAAGVLSAGHARAL
                     LGLTDAAAMERLAQRIVAEGLSVRSVEEIVAVGAVGTAPVKPKSPRAGGRRPQLDDMA
                     ASLSDHLDTRVTIALGQRKGKVVVEFASVEDLNRILDVIGVDRPKR"
     gene            3817..4488
                     /locus_tag="PLK64_08175"
     CDS             3817..4488
                     /locus_tag="PLK64_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV79761.1"
                     /translation="MAATPKRRKGGPEIVPVTADDVPALVEPCRDCAFWERGPGLVAG
                     TASTVSRDKAAWVRRVEAEWGTPGWLVRVDGQPAGHLLIAPAHLAPRSLAFPTSPVAD
                     DAILLLAVRVDPRYAGQGLGRRLVQTAAREVLSHDGRAIEAFGTTRDDQCLTPVDFLT
                     AVGFYVVREHAAYPRLRLDLRTALAWREDVEHALDRILAPVRRLGAGNPVGTANNEAP
                     GAGRP"
     gene            complement(4519..4845)
                     /gene="trxA"
                     /locus_tag="PLK64_08180"
     CDS             complement(4519..4845)
                     /gene="trxA"
                     /locus_tag="PLK64_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPV79762.1"
                     /translation="MGAIAATTDSTFATDVLANTKPVLVDFWAAWCGPCKQIAPILEE
                     IYAEYGDKIEIVKLDTDNNPATTARYGVTGIPTMNLYVGGEVVKTIVGAKPKRLLLRE
                     LEQFLG"
     gene            complement(4959..5948)
                     /gene="trxB"
                     /locus_tag="PLK64_08185"
     CDS             complement(4959..5948)
                     /gene="trxB"
                     /locus_tag="PLK64_08185"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPV79763.1"
                     /translation="MSDAVETATDTGVREVVIVGSGPAGYTAAVYAARANLHPVLFEG
                     SVTAGGALMNTTEVENFPGFEDGIQGPDLMFAMRAQAEKFGTEMVTDDIVAMDLAGDI
                     KTVTDGEGRVWRAKSVILAMGSAYRELGLPEEKRLSGHGVSWCATCDGFFFRDQDIAV
                     VGGGDSAIEEATFLTKFARSVTIIHRRDQLRASKIMADRALANPKIRFTWNAAVTALH
                     GDPKLTHVTLSDTVTGATSDLQVTGLFVAIGHDPRNELIRGVIDLDDAGYVVCEGRST
                     RTNLPGVFACGDLVDHTYRQAITAAGSGCAAALDTQHYLEALSDVAESVAAAH"
     gene            complement(6175..7146)
                     /locus_tag="PLK64_08190"
     CDS             complement(6175..7146)
                     /locus_tag="PLK64_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79764.1"
                     /translation="MSEPTRFDEDPDPTGVRILLASQPDPGPMPADVSDRILAAFAEA
                     GRRADLDADTGSQELSDELGLGVDAFDDFANLVAGQGVSGVDESGPAVPLHLVGRGTQ
                     PAGTSSETSGARTDVRTGSPSGSRSGAGMRGRVVDHRRRLTVLGGVAASVAALGLVGA
                     LLNNQGATSPTASSARAESVVGAAKDSSGLSATVDSAASANVPMAASSTGQPMHIELS
                     TRTYTKATLARLAQDMLDAPGRQLGQPAVEAPSVGPIGTLIGLTDCVTTLGESDADAV
                     FADLATFEGAPAVVIVVVDAELKHAYAVSRSCSKGDPGLLFGPVDMP"
     gene            complement(7271..7816)
                     /gene="sigM"
                     /locus_tag="PLK64_08195"
     CDS             complement(7271..7816)
                     /gene="sigM"
                     /locus_tag="PLK64_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigM"
                     /protein_id="HPV79765.1"
                     /translation="MTSGRVELAGRSDAELLAAHCAGDREAFAEIFRRHKDRMWAVAV
                     RTCGDPELASDCVQDAFIAAYRRAGSFRGESAVTTWLHRIVVNACLDRLRRTRPTSPL
                     PDYDLADRSDGHSAVEVRLDVRAALDQLPEGQRAALVLVDMHGLSVAETAEILGVAEG
                     TIKSRCARGRAAMAHLLGIRR"
     gene            complement(7813..8601)
                     /locus_tag="PLK64_08200"
     CDS             complement(7813..8601)
                     /locus_tag="PLK64_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79766.1"
                     /translation="MVAPRTLRAPDLIREDATLASVLAPVDSPLEDAVPLVPITGELG
                     RDESRLALAIVAGLVVLLAVLGIWGLPKLTSGGTTGGSVTTARVTRTATVTSSAGATG
                     AGATAGDGPAVVASLTPVAIIGAATFDASAGQVASSSAPRGYDGNAATMWRSKWYSNE
                     KFGGLKVPGIGYIVDLGQQTEVRRVTVTLPAPQDITVYVANRASLDGATAIGSSTGQS
                     GQLVFDAPAGPVSSGQLVIVFVTKLGPDGAGNFRAQVSEVAVSR"
BASE COUNT         1396 a         3223 c         3194 g         1395 t
ORIGIN      
        1 gcgatgagga tgacgccgac ggtggcgacg acgaggccag cggtccgtcc gcgggtcggt
       61 cgcgtggttc gcggtcgcgg tcccgcgtgg ctctgctcga gcgtgagggc gaggtagccg
      121 ccgacttcct ggagcggttg ctggacatcg ccgacctcga cggtgacatc gaggtcgaca
      181 tcgacgggga ccgcgctgcg gtgtcgatcg tcgactccga ggaaggccgc gttccgcgcc
      241 ggctggtggg ccctgacggc aaggttctgg atgccctcca ggagctcacc cggctcgccg
      301 tccagaccgc gaccggcgaa cgcagccggt tgatgctcga cgtggccggg ttccgggccg
      361 accgtcgcac cgcgctggtc accctggccc aagaggcgat cgccgaggtg cgcgcctcgg
      421 gtgtggcccg ggcgctggac aacatgtcgg ccttcgagcg caaggtcgtc cacgacgagg
      481 tgacggccgc cggcctggtc tcggaatccg aaggggtcga gccgcaccgg cacatcgtca
      541 tcagcccggc gtgaccgagg ttgatgtccc ggtcccgccg ctgtcggcgg cacaggtctt
      601 cggcccccgt gaagagctgg ccagggccta tgtcggtctg ctcgcggaca ccgggatcag
      661 ccacgggctg atcggcccac gcgaggtccc tcgcctctgg gaccgacacg tgctcaattg
      721 cgccgtcatc caggaactcc tgcccccgaa tgctctgtgc gccgacatcg gctcgggggc
      781 ggggctgcct ggtctggcgt tggcgatcgc tcggccggac atcaccatgc atctggtcga
      841 gcccctgcag cggcgggccc ggtggcttga cgtggctgtc gctgagctgg gtctgtcggg
      901 cgtgacggtg cattgctccc gggccgacgc gttgcacggc gtgctcaccg tggatgtcgt
      961 cacagcccga gccgtggccg agttggcgac cctgggtcag tggtgtctgc ccctactgcc
     1021 gcccggtgga cgtctgctgg ccatcaaagg gtccaaggct gaggccgagc tcgccgaggc
     1081 ttggccggcc ctgcagcgtc tggggtgcca ggccgggtcg gtccgtgagt gcggtgggtc
     1141 gatgatcgat ccgccgaccc gggtcgtgga ggtgattgcg gggtctgcgc catcgtcggg
     1201 caaaggacgg gcccgcccac ccagcgcccg cgcgcaacaa cgcaaggcgc gagccgccgc
     1261 agcgagacgg caccgtggag ggttctcgcc caccggtgcc gatgaggcat caccgagcaa
     1321 ctgacgccca ccagcaccga tctcgcccac tacgggcctg gcctgtggat gatggcgaaa
     1381 gccgaggcgg gcgctgtggt tggatgcagg gatggagcgg cgcgcacgga ccagtcgggg
     1441 actggggtgg ccttggggtt catcccctgg ccggcgaagc cctctggggt ggcccgacca
     1501 cggttcggac gatgatccgg gtgaccagca gccgttgacc agcgactcgg acccgacggt
     1561 cgatgtttca cgtgaaacac ctgacccgtt gcttggttcc gcgacccctc cggaccagac
     1621 ggacctggcc tcagccgacg acgttgtttc acgtgaaaca cccgaccggg agcttggttc
     1681 cgcgacccct ccggaccaga ccgatctggc ctcagccgac gacgttgttt cacgtgaaac
     1741 acctgacccg gcagctgaga gcagcagcct cgtggacctc caggacgaga gcaccccgct
     1801 cgcggctgcc atcgcccaag atgtgcgccg tcggtccacc ctcgtcggag cccccctgcc
     1861 gcgccccccg gccacccgag ttctcaccgt cgccaaccag aagggtggcg tgggcaagac
     1921 cacctcagcg gtgaacgtcg cggccgccct tgcgcagtcg gggatgcggg ttctcgtcat
     1981 cgacctcgac ccccaaggca acgcgagcac ggcattgggg gtggaccacc gggccggcat
     2041 cgactcgatc tatgacgtcc tcgtggacgg ccaaccgatg agcgcggtcg tcgtcgaatc
     2101 ccccaccgtg ccgggactct tctgcgcccc gtcgacgatc gacttggccg gcgccgagat
     2161 cgagctcgtc tcgctcgtcg cccgtgaggg ccgcctggcg acggcgctca atgcgcacct
     2221 ggccgacacg gcatacgact atgtcttcat cgactgtccc ccgagtctcg ggctgttgac
     2281 cgtcaacgcc ttcgttgccg cgcgcgaagt gttcatcccc atccaatgcg agtactacgc
     2341 gctggagggc ttgtcccagt tgctgcgcaa cgtcgagttg atcaggacgc atctcaaccc
     2401 cacgctcgcc gtctcgacca tcctgttgac gatgtttgac ggtcggaccc ggctgtcggc
     2461 ccaggtggcc gaagaggtgc gagagcactt ccccgagcag accctgcgca cgaccatccc
     2521 ccggtcggtc cgggtgtcgg aggctcccag cttcggcgag accgtcatga cctatgaccc
     2581 gttgagcagt ggggcgctgg cgtatctcga agccgccagt cagatcgccc agcgaggtgc
     2641 cgaatcgatg aacgagggag cacatccgtg aaggaacgag agaagcgacg cgcgttgggc
     2701 cggggcctgg gggcgctcat ccccacggcc cccgcggggg agcggcccgt cgacgtcttc
     2761 ttcgggcctg cgccgtcagc aatcaccgtc gacattggca cgaagcacgc cgcggccgac
     2821 ggcgacccga gcgacctgac tgattccgcc gagaccaccg acccgaccga cgcggctgcc
     2881 gcgtcgtcgt cgagtagccc cacggacgcc ccctcggcag gcaccggacc ggcagacccg
     2941 gacaacgacg ccctgcgacc ggtccctggg gccacgttcg ccgaactccc cgtcgagtcg
     3001 atccggccca acccacgcca gccacgcacc gtcttcgacg aagacgacat ggcggagttg
     3061 gtccactcgg tgagtgagat cggcatcctt caaccgatcg tcgtgcgccg agtcgacgat
     3121 ggtgggcccc tcggctcggg acacgacgct gctatcgcga cctatgagct ggtcatgggg
     3181 gagcggcggt ggcgagccgc gcaggcagcc ggcctcgaac atgtgccggc gatcatccgc
     3241 tccacccacg acgacgacct gctgcgcgat gcgctcctgg agaaccttca ccgcagccaa
     3301 ctcaacccgt tggaagaggc ggcggcctat cggcaactcc tggatgactt cggctgcacc
     3361 caagaggagt tggccacccg gatcgggcgt agccgcccgc aggtgagcaa caccctgcgg
     3421 ctgctcaaac tgccaccgct ggtccagcgc cgcctcgcgg ccggggtgtt gagcgccgga
     3481 catgcccgcg ccctccttgg cctcaccgac gccgcggcca tggagcgctt ggcccaacgg
     3541 atcgtcgccg agggcctctc agtccgctcc gtcgaggaga tcgtcgccgt cggggcggtg
     3601 ggaacggctc cggtcaaacc caagtctccc cgagccggtg ggcggcggcc gcaactcgac
     3661 gacatggccg cgtctctgtc cgaccacttg gacacccggg tgacgatcgc cttaggacaa
     3721 cgcaagggca aggtcgtcgt ggagttcgcc tcggtggagg acctcaaccg gatcctggac
     3781 gtgatcggcg tcgaccgacc caagcgctga ccggacatgg ccgcgacacc caagcgccgc
     3841 aagggcggtc cggagattgt gccggtgacg gccgatgacg tgcccgccct cgtcgagccg
     3901 tgtcgtgact gcgccttctg ggagcgtgga cccggcctcg tcgcggggac ggcgagcaca
     3961 gtgagcaggg acaaggctgc gtgggtgcgc agagtcgagg ccgagtgggg caccccgggg
     4021 tggctggttc gggtggacgg tcagccggct ggtcaccttc tcatcgcccc ggctcatctc
     4081 gcgccccgct ccctcgcctt cccgacctcc ccggtcgccg acgatgcgat tctcctgctc
     4141 gcggtgcggg tcgatccccg gtatgccggt cagggcctgg ggcgccgact ggtccagacc
     4201 gccgcgcggg aagtcctctc ccacgacggt cgggcgatcg aggccttcgg aaccacccgg
     4261 gacgatcagt gcctgacccc ggtcgacttc ctcaccgccg tgggcttcta cgtcgtgcgg
     4321 gagcacgcgg cataccctcg gttgcggctg gatctgcgca ccgcactggc ctggcgcgag
     4381 gacgtcgagc atgcgctcga tcggatcctc gctccggttc ggcgcctggg ggccggcaac
     4441 cccgtcggca ccgcgaacaa cgaagcgccc ggcgctggtc ggccctgagg ccgccgacac
     4501 cgggcgctgc gatgaggctc agccgaggaa ctgctccaac tcacgcagca acagccgctt
     4561 gggcttggcg ccgacgatgg tcttgacgac ctcgccgccg acgtaaaggt tcatcgtcgg
     4621 gatgccggtg acgccatagc gcgcagtcgt cgccgggttg ttgtcggtgt cgagcttgac
     4681 gatctcgatc ttgtcgccgt actcggcgta gatctcttcg aggatcgggg cgatctgctt
     4741 gcacggcccg caccaggcgg cccagaagtc aacgaggaca ggcttcgtgt tcgcgagcac
     4801 gtcggtggcg aaggtggagt cggtggtcgc ggcaatggcg cccatgtgag gtcctttcac
     4861 ggtgtgcggc ccacggactg gtatgccgtg cgcccggtct gggtctgggt catggtctgg
     4921 tcgaaaacta gcgcgggggt ctgacagtcg gtgctggctc agtgcgccgc agcgactgac
     4981 tcggcgacat cggacagagc ctcgaggtag tgctgggtgt cgagggcggc cgcacagcca
     5041 ctcccggcag cagtgatggc ctggcggtag gtgtggtcga cgaggtcgcc gcaggcgaag
     5101 acgcccggca ggttggtgcg agtcgatcgg ccctcacaga cgacgtagcc tgcgtcgtcc
     5161 agatcgatga ccccgcggat caactcgttg cgggggtcgt gaccgatggc gacgaacagg
     5221 ccggtgactt gcaggtcgct ggtggcaccg gtgacggtgt cggagagggt cacgtgggtg
     5281 agtttcgggt cgccgtgcag ggcggtgacg gcagcgttcc acgtgaaacg gatcttggga
     5341 ttggccagcg cgcggtcggc catgatcttc gacgcccgca actggtcgcg ccggtgaatg
     5401 atcgtcacgc tccgggcgaa cttggtgagg aaggtcgctt cctcgatcgc ggagtcacca
     5461 ccgccgacga cggcgatgtc ctggtcgcgg aagaagaacc cgtcgcaggt ggcgcaccac
     5521 gacacaccgt ggccggacaa gcgcttctcc tcgggcaggc ccaactcgcg gtaggccgat
     5581 cccatcgcca gaatgacgct cttggcgcgc cacacccggc cttcaccgtc ggtcaccgtc
     5641 ttgatgtcgc cagcaaggtc catggcaacg atgtcgtcgg tgaccatctc agtgccgaac
     5701 ttctcggcct gcgcgcgcat ggcgaacatc aggtcggggc cctggatgcc gtcctcgaag
     5761 ccggggaagt tctcgacctc ggtggtgttc atcaaggcgc cgccggcggt gaccgagccc
     5821 tcgaagagca cagggtgaag gttggcgcgg gcggcataca ccgcggccgt gtagcccgcg
     5881 ggaccggacc cgacgatcac gacctcgcgc acacccgtgt cggtcgcagt ctcgacagcg
     5941 tcactcaccg gaactcctcg atcgtttgcg gcgcttcacc gctgtgggct cgcgacacgg
     6001 cacatgtggt gtcgtcgcgg ggcaggagac ccaagatacc cgcccaatcc ccccaaccat
     6061 cgcggcgcac cgcagattcc ccgacggttc acctcagggg tatgccgtcc gctcaccgac
     6121 cgccgtcgcg gaaccattgg acgtcacgat cgcccctcat cgtgtgccag caggtcaggg
     6181 catgtcgacg ggaccgaaga gcagccccgg gtcgcccttg ctgcaactgc gactaacggc
     6241 atacgcgtgc ttgagctcag cgtcgacgac gacgatgacc accgcggggg cgccctcgaa
     6301 cgtggccaga tcggcgaaca ccgcgtcggc atcgctttcg cccagggtgg tcacgcagtc
     6361 ggtcagaccg atcagggtgc caatgggccc caccgagggc gcctcgacgg cgggttgccc
     6421 caactgccgg cccggcgcgt cgagcatgtc ctgcgcgagg cgggcgaggg tggccttcgt
     6481 gtaggtgcgg gtgctcaact cgatgtgcat cggctgaccg gtggagctgg ccgccatcgg
     6541 cacgttcgcg ctggccgctg agtcgacggt cgcactaaga ccgctggagt cctttgcggc
     6601 ccctacgacc gactcggcac gcgcgctcga cgcagtaggc gatgtggcgc cctggttgtt
     6661 gaggagggcg ccgaccaggc ccaaggcagc aacggaggcg gcaacacccc cgaggacggt
     6721 caaccgccga cgatgatcga cgacccgccc gcgcatacct gcgcccgagc gcgaccccga
     6781 gggtgacccc gtgcgcacgt cggtccgagc acccgacgtc tcagatgatg ttcccgctgg
     6841 ctgcgtccca cgcccgacga ggtgcaaagg caccgccggc ccggactcgt caactcccga
     6901 aaccccttgg cccgcaacaa gattcgcaaa gtcgtcaaac gcgtcgacac ccaggccgag
     6961 ttcgtcgctc aactcttgcg agccagtgtc ggcatcgagg tcggcgcgtc gaccggcctc
     7021 ggcgaaggca gcaagaatcc ggtcggacac gtccgccggc atcggccccg gatcgggctg
     7081 ggaggcaagc aggatccgca ccccggtcgg atcagggtcc tcgtcgaacc tggtcggctc
     7141 gctcatcaac actccctcgg gatgtggctc gtctggcccg ccccccgacg gaccggcccg
     7201 cagctgggca tgggtttcag gactgagacg cacgccggga cgcgctggtt cccgcggcat
     7261 accgtggcgg tcatcggcga atccccagca agtgcgccat cgcggcccgc ccgcgggcgc
     7321 accgtgactt gatggtcccc tcggccacgc cgaggatctc ggcggtctcg gcgacggaca
     7381 ggccatgcat gtcgaccagc acgagcgcgg cccgctgacc ctccggcagt tggtcgaggg
     7441 cggcgcgcac gtccaggcgc acctcgacgg cagagtggcc gtcgctgcga tcggccaggt
     7501 cgtagtcggg cagcggactc gtgggacggg tgcggcgcag ccggtcgagg caggcgttga
     7561 caacgatccg atgcagccac gtggtcactg cggactcccc gcggaaggac cccgctctcc
     7621 ggtatgccgc gatgaaggcg tcctggacgc agtccgaagc gagctcgggg tcaccgcagg
     7681 tgcgcacggc gaccgcccac atccggtcct tgtggcgtct gaagatctcg gcgaaggcct
     7741 cgcggtcgcc cgcgcagtgt gcggccagca actcggcgtc ggagcgcccg gccaactcca
     7801 ctcgacccga ggtcaccggc tgaccgccac ttcggacacc tgggcccgga agttgcccgc
     7861 gccgtcgggt ccaagcttgg tgacgaagac gatgacgagt tggccgctgg acacgggccc
     7921 ggccggggca tcgaagacga gttggccgct ctggccggtg ctggagccga tcgccgtggc
     7981 cccgtcgagc gaggcccggt tggcgacgta gaccgtgatg tcttgcgggg caggcagcgt
     8041 gacggtcacc cggcggacct ccgtctgctg gccgaggtcg acgatgtagc cgatcccggg
     8101 caccttgagg ccgccgaact tctcgttgga ataccacttc gagcgccaca tcgtcgcggc
     8161 gttcccgtcg taacccctcg gtgcgctgct cgatgccacc tgcccggcgc tcgcatcgaa
     8221 agtggcggcc ccgatgatgg ccacgggggt cagcgaagca acaaccgcag ggccgtcgcc
     8281 ggccgtggcc ccggctccgg tcgcgcccgc gctcgacgtg acggtcgccg tcctcgtgac
     8341 cctcgcggtc gtgaccgacc cgccggtcgt cccacccgac gtcagcttgg gcagccccca
     8401 gatccccagc accgccagga gcacgaccag accggcgacg atcgccagcg cgaggcggga
     8461 ctcgtcgcgc cccaactcgc cggtgatcgg aacgagcggg accgcatcct cgaggggact
     8521 gtccaccgga gcgaggaccg aagccagcgt cgcgtcctcg cggatcaggt caggggcccg
     8581 cagcgtccgc ggcgcgacca ccctcggcgc tccaggcgcc acgcggggcg ccgagggccc
     8641 aggtgccgtc ggcgtgccgc tgccgggcga gccgggaggc tgcgcggacg aggcttcggg
     8701 cgctcccgct cctggtgcgg cgggggcgga cgcgatcccc tcatcggtgg ccggttcggg
     8761 tcgggttgag agccgttcga cggtatgccg tgcccgccca cccaggcgtg acgcggcctg
     8821 agacgctgcg gtgccagcgg caccgagggc cgaccccacg ccccgggctc ccttggccat
     8881 agcggcactg accgcggcag cagtcgccgc ggcactcgtg gcacctgcgc ctccggcggc
     8941 tccgccagcc ccggtggcca acgagacgcc ctcggccgtg gcgccaccgc cagcactgga
     9001 cgacggttgc tgctgcgacg aaaagggctg cgctgcaggg ctatcgggct gaacgaggct
     9061 gggcgctgct ggacccgcgc ctgcagctgc gcctccaccc gctcctgcac cctgcccggc
     9121 actggggcct gcaccctggc cttcggcggc gctggggacc gccggtgggc gccggtcacc
     9181 ccagagatcg accgtcggcc ccttgggc
//
LOCUS       DAOMZY010000120         9194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_7126_length_9194_cov_5.828975, whole genome shotgun sequence.
ACCESSION   DAOMZY010000120 DAOMZY010000000
VERSION     DAOMZY010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9194
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7126_length_9194_cov_5.828975"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLK64_08205"
     CDS             complement(<1..244)
                     /locus_tag="PLK64_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79767.1"
                     /translation="MSEAGQDVTELVEASEPVAEVDETVVSEVELDEVEVQGESDSGD
                     VPVGDESDDDSDDDSDDDSDDDSDDDEESDDDSDEES"
     gene            complement(302..1357)
                     /gene="yidC"
                     /locus_tag="PLK64_08210"
     CDS             complement(302..1357)
                     /gene="yidC"
                     /locus_tag="PLK64_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HPV79768.1"
                     /translation="MGDFINLILSPLTIGEAWVMLAWHKVWTTIGIGGGWAWALSIVG
                     LTVVVRIVLIPLFVKQIHSSRRMQLIQPEMQKIQAKYKGKTDPESRQAMTQETMDLYK
                     KTGTNPFSSCLPILLQSPFFFALFTVLNNLKGIAEGTHPAIGPITPAVAHEIESSNIF
                     GAPFSSTFMGSTDTATKIVTVVLIILMSVSTFTTQRQLMRKNLPDSALDNPFMKQQTV
                     LLYLMPIFFALSGINFPIGVLLYWLTTNVWSMGQQFYVIRRMPAPGSAAERALHERQE
                     RKGKTVTPFSIRGLERDAAAGAAADETVQDAPNTVNRQRQQPKSKKRAKRSGPATGAA
                     GTDDAEGSAADGKPTDQ"
     gene            complement(1449..1751)
                     /gene="yidD"
                     /locus_tag="PLK64_08215"
     CDS             complement(1449..1751)
                     /gene="yidD"
                     /locus_tag="PLK64_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPV79769.1"
                     /translation="MTLAELLGPGLRGPWTWRKAAAAPLLMLVRIYQLAISPLLPQRC
                     RFYPSCSAYAVTALTRFGPLRGGWLAARRLGRCHPWNPGGVDHVPEREACRTHAAT"
     gene            complement(1756..2112)
                     /gene="rnpA"
                     /locus_tag="PLK64_08220"
     CDS             complement(1756..2112)
                     /gene="rnpA"
                     /locus_tag="PLK64_08220"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPV79770.1"
                     /translation="MLPARNRLRESGQFRAVLRGPGGSRAGGPLMVVHAAMTDPMGAR
                     APRVGFVVSGAVGGAVVRNRVKRRLRALAGTALGGIPAGCDLVVRAAPAAATASFAEL
                     QAELDRCLRRALAKVA"
     gene            complement(2122..2259)
                     /gene="rpmH"
                     /locus_tag="PLK64_08225"
     CDS             complement(2122..2259)
                     /gene="rpmH"
                     /locus_tag="PLK64_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013428281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPV79771.1"
                     /translation="MSKRTFQPNNRRRAKTHGFRLRMRTRAGRAILAARRRKGRSELS
                     A"
     gene            2722..4194
                     /gene="dnaA"
                     /locus_tag="PLK64_08230"
     CDS             2722..4194
                     /gene="dnaA"
                     /locus_tag="PLK64_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPV79772.1"
                     /translation="MTGGDVDLTDLWQRTLVALERTGVSPSERAFVGFARLMAVVEQT
                     ALIAVPNDYTKKVLESDVREHLVATLSGALGRDIGLAVTVDPTLEHATPSSNGVDPAN
                     GFDTANGDERHESPDAARMERFHPEGSGRHTPGVIAFPRRDGDQDRSRLNPKYTFDTF
                     VAGSSNRFAHAAANAVSEAPAKAYNPLFIYGSSGLGKTHLLHAIGHYVRQIYPGFRVR
                     YVNSEEFTNEFINSIRDDRASTFQSLYRDVDVLLIDDIQFLAGKVQTQEEFFHTFNAL
                     HNNSKQVVITSDLPPKQLSGFEERLRSRFEWGLITDVQAPDLETRIAILRKKTLQERM
                     SAPDEVLEYIATRITSNIRELEGALIRVTAFASLNRQPVDFTLAEVVLKDLVPTDQPV
                     QITIGTIQAETASYYALTVDDLCSSSRSRQLVTARQIAMYLCRELTELSLPKIGQAFG
                     GRDHTTVMHADRKIRELMSERRAIYNQVTELTARIRNHSR"
     gene            4706..5839
                     /gene="dnaN"
                     /locus_tag="PLK64_08235"
     CDS             4706..5839
                     /gene="dnaN"
                     /locus_tag="PLK64_08235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013490886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPV79773.1"
                     /translation="MKFRVEREVLADAVAWVARGLPNRPPVPVLSGVLIEADRAGTVT
                     LSAFDYEVSAKVTVAADVDEGGTVLVTGRLLSEISRALPARPVDVSTEGNKVTVTCGT
                     SRFSLLMMQADDYPTLPAAPQPSGTIAGDVFTQAVHQVAIAADRGDTLPILTGVRMQI
                     EGDKVTLLATDRYRLAMRELTWAPARSDAAYTALIPARTLSDTAKALGAAGSVELALG
                     GNAGGDGLIGFEAGQRRATTRLLDGEYPKVTSIFPTSVDSEAVLETGALIEAVKRVAL
                     VAERNTPVRLRFTDGQVAIEAGTGDDAQASEAVESTLTGPDIEIAFNPHFLLDGLGVI
                     GTPFTRFSFTQPSRPAVMAGQREATGEADTSYRYVLMPVRFAS"
     gene            6046..7068
                     /gene="gnd"
                     /locus_tag="PLK64_08240"
     CDS             6046..7068
                     /gene="gnd"
                     /locus_tag="PLK64_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HPV79774.1"
                     /translation="MQLGLIGLGRMGANMRQRIRAGGHEVIGYDKNPAVRDVASLATM
                     VKKLSGPRVVWIMVPAGAPTRDTVAKLAGLLEKGDLVIDGGNSRFTDDFANAKLLAQK
                     GIGYVDCGVSGGVWGLENGYGLMAGGDKKWINLAMPIFDALRPEGERDLGFVHAGEVG
                     AGHYAKMVHNGIEYAMMHAYGEGYELLAKKDIVTDVPGCFKAWSRGTVVRSWLLDLLV
                     DALEDNPTLADVSDYTNDSGEGRWTVEEAIDNAVPMPVIAASLFARFASRQPVSPALQ
                     AVAALRGQFGGHAVMTVAEGENLRATAPESAGAKASPGQAPAAAKVAAKKTTTRRAAP
                     KASPAH"
     gene            7097..8308
                     /gene="recF"
                     /locus_tag="PLK64_08245"
     CDS             7097..8308
                     /gene="recF"
                     /locus_tag="PLK64_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HPV79775.1"
                     /translation="MHVRHLSLVDFRSYAVAELPLAPGVTTIMGLNGQGKTNLVEAIG
                     YVATLGSHRVATDQPMVRFGAAQTVIRAAIVRHDRETLVELELTPGRANRARLNRAPL
                     TRTRDVLGTLRTVLFAPEDLALVKGDPAGRRAFLDELLVARQPRWAGVRADYERILKQ
                     RNALLKSAAPVLRKGSSRRRSASLAPGENPDTATESTLHTLDIWNTHLAEVGAQLLYA
                     RLRLLRDLTPFLAAAYATVSAGVTPVSASYRASLGESVTAAIGAGEVPDLEDLRAELL
                     AAFEANRAAEVERGVTLVGPHRDDVHLALGPMPAKGYASHGESWSLALGLKLAAYQLL
                     RRDLGEDPVLVLDDVFAELDTQRRERLAELVADCEQVLVTAAVGADVPERLRSTGRTY
                     AVTLGEVTRVD"
     gene            8481..8900
                     /locus_tag="PLK64_08250"
     CDS             8481..8900
                     /locus_tag="PLK64_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HPV79776.1"
                     /translation="MKPAPNSRRIAGMPPTRSAGDDRDPALLGAQLERLLLDRGWSAD
                     VTVGSVMGRWAEIVGAEIAGHVRPVTFEGTVLSVQADSTAWATQIRLLTSSLLGRIEA
                     VVGAGIVTDLVVLGPAAPSWVKGRRRVQGPGPRDTYG"
BASE COUNT         1483 a         3267 c         3023 g         1421 t
ORIGIN      
        1 ctgactcctc atcggagtca tcgtccgact cttcgtcgtc gtccgagtcg tcgtcggagt
       61 cgtcgtccga gtcgtcgtcg gagtcatcat cagactcatc gccgaccggc acgtcgcccg
      121 agtcggactc accctgcacc tcgacctcgt caagctcgac ctcgctaacc acggtctcgt
      181 cgacctcggc caccggttcg ctcgcctcga cgagctccgt cacgtcctgc cctgcttcgc
      241 tcattgacac ttctccttcg tcagccgccg ccgcgcgtat gccgcgctgg cacggcatac
      301 ctcactggtc tgttggcttg ccgtctgccg cggatccctc ggcgtcgtcg gtgccggctg
      361 cgccggtggc cgggccggaa cgcttggccc gcttcttgct cttgggttgc tgtcgctggc
      421 ggttaaccgt gttgggtgcg tcctgaaccg tctcgtcagc tgcggcccct gcggcggcgt
      481 cgcgctccag ccccctgatc gagaaggggg tcacggtctt gcccttgcgc tcctggcgtt
      541 cgtgcagcgc ccgctcggcg gccgagcccg gcgccggcat ccggcggatg acgtagaact
      601 gctggcccat cgaccacacg ttggtcgtca gccagtagag gagaacgccg atcgggaagt
      661 tgatgcccga gagggcgaag aagatcggca tgaggtagag caacaccgtc tgctgcttca
      721 tgaacgggtt gtccagcgcc gagtcgggca ggttcttgcg catcaactgc cgctgcgtcg
      781 tgaacgtcga caccgacatg aggatgatga ggacgaccgt gacgatcttc gtggccgtgt
      841 cggtcgaccc cataaaggtc gaggagaacg gcgccccgaa gatgttcgac gactcgatct
      901 catgggccac cgctggcgtg atcggcccaa tcgccgggtg ggtcccctcc gcgatgccct
      961 tgaggttgtt gagcaccgtg aacagcgcga agaagaacgg cgactgcagc aggatcggca
     1021 gacacgagga gaacgggttg gtcccggtct tcttgtagag atccatcgtc tcctgagtca
     1081 tcgcctgccg agactcggga tcggtcttgc ccttgtactt cgcctggatc ttctgcatct
     1141 ccggctggat caactgcatc cgccgggacg agtggatctg cttgacgaag agcgggatga
     1201 gcacgattcg cacgacgacc gtcagcccga cgatcgacaa ggcccaggcc cagccaccac
     1261 cgatgccgat cgtcgtccag accttgtgcc aggcaagcat cacccaggcc tcgccgatgg
     1321 tcagcggcga gaggatgagg ttgatgaagt cgcccactgt cgtccttgct cgaaaggcga
     1381 gactctcatc tcgccggatg gttcggtccg gtcagcagtc caggtatgcc gctcgcggac
     1441 caggtggtct aggtcgccgc gtgagtgcgg caggcttccc gctcgggaac gtggtcaacg
     1501 ccgccagggt tccacgggtg gcagcggccc agccggcgcg cagccagcca gccaccgcgc
     1561 agcggaccga accgcgtgag ggcggtgacg gcatacgccg agcaggaggg gtaaaagcgg
     1621 caccgttgcg gcaacagcgg cgagatcgcc agttggtaaa tccgaacgag catgagcagg
     1681 ggcgccgcag ccgccttgcg ccacgtccac ggaccgcgca atccgggccc gagcagctct
     1741 gccagcgtca tcagctcacg ccaccttggc cagggcgcga cgcaggcacc gatccaactc
     1801 ggcctgcaac tcggcgaacg acgctgtcgc cgcggccgga gccgcccgca cgaccaggtc
     1861 acacccggcc ggtatgccgc ccagcgcagt cccggccagc gcgcgcaacc ggcgcttgac
     1921 ccgattacgc acgacggcgc ctccgacggc gccggacacg acgaaaccga cccggggggc
     1981 gcgagcaccc atcggatcgg tcatcgctgc gtgaaccacc atcaacggtc cgccggctcg
     2041 cgacccacca gggccgcgga ggacggcacg gaactggccc gactcacgca gccgattgcg
     2101 ggccgggagc acatatccgc ctcaggcgga cagctcggag cggcccttgc gacggcgagc
     2161 ggcgaggatc gcgcgaccgg cgcgcgtccg catccgcagg cggaagccgt gcgtcttggc
     2221 gcgacggcgg ttgttcggct ggaaggtgcg cttgctcacg ggtctgtctc cgtcgatcgg
     2281 caggcccagg gcgggtagcc tgcgggcgga ctgcggtgga ctgcggggcg cccgtatgcc
     2341 gcgtcacccc gtccgccggc cgcaatcgga agcccgcggt gacaagcacg ggcgcgcgaa
     2401 agcggccgga gtcagagtcg acacatgggc cgcagatcag ggtacggccc gtcggcgggc
     2461 agggtcaaac ccgggacctc gaccagcaac gaaaacccag cattctcccc cctcgtcatt
     2521 ccggtttagg ggagcgacac gcccatcaag gggtgtaatc tctccacgca gcccccggac
     2581 agacgatgct tccggctgcg aggccttccc ccatcggtgg tttgtcccca ccctgtggat
     2641 agagctgtgg atgacgggta atgtcgaacg tctggaggcc cgatccatcg ggcccggcgt
     2701 cggcaaggag gggagccaca cgtgacaggc ggagacgtcg atctcaccga cctgtggcaa
     2761 cgcaccctcg tggctctgga acgcaccggt gtctccccca gtgagcgtgc cttcgtcggt
     2821 ttcgcccgcc tgatggccgt cgtcgaacag accgccctca tcgccgtgcc caacgactac
     2881 accaagaagg tgctcgagtc ggacgtccgc gagcacctcg tcgcgacgct cagcggagcg
     2941 ctcggccgcg acatcgggct ggccgtcacg gtcgacccca ccctcgagca cgccaccccc
     3001 agctccaacg gggtcgaccc cgccaacggg ttcgacactg ccaacggcga cgagcgccac
     3061 gagagccccg acgccgcgcg gatggaacgc ttccatcctg aggggagcgg gcggcatacc
     3121 cctggggtga tcgccttccc ccgacgcgac ggtgaccagg accgcagccg gctcaacccg
     3181 aagtacacct tcgacacctt cgtcgccggg tcgagcaacc ggttcgcgca tgccgcggcc
     3241 aacgccgtgt ccgaggcgcc ggccaaggcc tacaacccgt tgttcatcta cggctcctcg
     3301 gggctgggca agacccacct gctgcacgca atcggccact acgtccgcca gatctatccc
     3361 ggcttccggg tgcgctacgt caactccgag gagttcacga acgagttcat caactcgatc
     3421 cgtgacgacc gggcgagcac cttccagagc ctctaccgcg atgtcgacgt gctgctcatc
     3481 gacgacatcc agttcctcgc cggcaaggtg cagacgcagg aggagttctt ccacaccttc
     3541 aatgcgctgc acaacaacag caagcaggtc gtcatcacca gtgacctgcc gcccaagcaa
     3601 ctcagtggtt tcgaggagcg gctgcgtagt cggttcgagt ggggcctcat caccgacgtg
     3661 caggcacccg acctcgagac ccgcatcgcg atcctgcgca aaaagaccct ccaggagcgg
     3721 atgagcgccc cggacgaggt gctggaatac atcgccaccc ggatcacctc gaacatccgc
     3781 gagctcgaag gggccctcat ccgggtcact gcgttcgcca gcctcaaccg gcagccggtc
     3841 gacttcaccc tcgccgaggt ggtcctcaag gacctcgtcc ccaccgacca gccggtgcag
     3901 atcaccatcg ggaccatcca ggccgagacg gcgagctatt acgccctcac cgtcgatgac
     3961 ctgtgcagtt cttcgcgctc gcggcagttg gtgacggccc ggcagatcgc catgtatctg
     4021 tgccgcgagc tgaccgagct gtcgctgccc aagatcggcc aggccttcgg cggccgcgac
     4081 cacacgaccg tcatgcacgc cgatcgcaag atccgcgagc tgatgagtga acgtcgggcg
     4141 atctacaacc aggtcaccga gctcaccgcg cgcatccgca accactcgcg ctgacccatt
     4201 cacttcggca cgctccgtcg cgtcacgctc ctcccgcctc acgctccccc cctgcagctt
     4261 cctctgccct gagccctccc ctaagttgtc cacactcggc tgtggacagc tgtggacaac
     4321 gtccaccatt tgggggacaa caacaccctc acagtgcact ggctgtgggt ccgtggtgcc
     4381 cgtcaacagg ctcggtgagt tacccaccga ctcatgcacg cggcataccc aggccgcttg
     4441 ggccgctgac ctgcggactt gagggttatc cacagaatcc acaacaccta tgacgacgac
     4501 gagtccacca attctctcca atgccccgac aacgctgggg atcaggcagg agcgtgcgca
     4561 actgcggtga cccttccctc ttgcagttcc cgcccaaccg actagggtcg gagttccccc
     4621 cgacacggcg gtggtgcgcg cgcccgcgcc gttctgccgg ttgagccggg gtctggatcg
     4681 aagaagcacg agggtggaga cgatcgtgaa gttccgcgtt gagcgcgagg tgctcgctga
     4741 tgccgtggcc tgggtggctc gcggtctgcc gaaccgacct ccggtgcccg tcctgtccgg
     4801 cgtgctgatc gaagccgatc gggccggcac ggtcacgctg tcagccttcg actacgaggt
     4861 gtccgccaag gtcaccgtcg ccgccgacgt tgacgaaggc ggcaccgtgc tggtcaccgg
     4921 gcgcctgctc tcggagatct cgcgcgccct ccccgctcgc ccggttgacg tctcgaccga
     4981 gggcaacaag gtcacggtca cctgcgggac gagccggttc agcctgctga tgatgcaggc
     5041 cgacgactac cccaccctgc cggccgcgcc gcagccctcc ggcaccatcg ccggcgacgt
     5101 gttcactcaa gccgtgcacc aggtcgccat cgcggccgac cggggtgaca cactgccgat
     5161 cctcaccggc gtccggatgc agatcgaggg cgacaaggtc accctgctgg cgaccgaccg
     5221 ctaccgcctg gccatgcgcg agctcacctg ggcaccggcc cggtccgacg cggcatacac
     5281 cgcgctcatc ccggcccgca ccctcagtga caccgcgaag gccctcggtg ccgccggctc
     5341 ggtcgagttg gccctcggag gcaacgccgg tggcgacggg ctgatcggtt tcgaggcggg
     5401 gcagcggcgc gcgacgactc ggttgctcga cggcgaatac cccaaggtca cctcgatctt
     5461 cccgacctcg gtcgactccg aggccgtgct cgagaccggc gctctcatcg aggcggtcaa
     5521 gcgcgtggcc ctcgtcgccg agcgcaacac gcccgtgcgg ttgcggttca ccgatggtca
     5581 ggtggccatc gaggccggca ccggtgacga cgcgcaggcc tccgaagcgg tcgagtcgac
     5641 gctcaccggg cctgacatcg agatcgcgtt caacccgcac ttcctgctcg acggtctcgg
     5701 cgtcatcggc acccccttca cccgcttctc cttcacccag cccagccgcc ccgcggtcat
     5761 ggctgggcag cgggaggcga cgggtgaggc cgacacgtcc taccgctacg tcctcatgcc
     5821 cgtccgcttc gccagctgag gcttcacggg tgggagggcg ggcatggagc cgctcatgaa
     5881 gccgcagcag gtggtgtgat aacaccatca gggacggctt catgggcgac ttcacagccg
     5941 tcgcagcgcg cgcctggcac gcggcatacc tgagccactg gtgagcgggg catagcctga
     6001 cctccgcagc gtccctatcg accgtcactt caggaggcac ccgtcatgca actcggtctc
     6061 atcggtctgg gccggatggg cgccaacatg cgccagcgca tccgggcggg cggccacgag
     6121 gtgatcggct acgacaagaa cccggccgtg cgcgacgtcg ccagcctggc cacgatggtc
     6181 aagaagctga gcggtccccg ggtcgtgtgg atcatggtgc ccgcgggcgc gccgacgagg
     6241 gacacggttg ccaagctggc cgggctgctc gagaagggtg acctggtcat cgacggcggg
     6301 aactcccgct tcaccgacga cttcgccaac gcaaaactgt tggcgcagaa ggggatcggt
     6361 tacgtcgact gtggcgtctc cggtggcgtg tgggggctgg agaacggcta cggcctgatg
     6421 gctggcggcg acaagaagtg gatcaacctc gcgatgccga tcttcgacgc gctgcggccc
     6481 gagggcgagc gcgacctggg gtttgtccac gcgggcgaag tcggcgcggg gcactacgcc
     6541 aagatggtcc acaacggcat cgagtacgcc atgatgcacg cctatggcga gggctacgag
     6601 ctgctggcca agaaggacat cgtcaccgac gtgcccggct gctttaaggc ctggagccga
     6661 gggacggtcg tgcggtcctg gctgctggac ctgctcgtcg acgccctcga ggacaacccg
     6721 acgctggccg acgtctcgga ctacaccaac gactccggcg aaggtcgctg gaccgtcgag
     6781 gaggcgatcg acaacgccgt cccgatgccg gtcatcgccg cgtcgctctt cgcccggttc
     6841 gcctcgcgcc agccggtgtc gccggccttg caagccgtgg ctgcgttgcg cgggcagttc
     6901 ggtgggcacg cggtgatgac ggtggctgag ggcgagaacc tgcgcgccac cgcgcccgag
     6961 agcgccggag ccaaggcgtc accgggtcag gcaccggctg cggctaaggt cgctgccaag
     7021 aagaccacca cgcgccgggc cgcccccaag gcatcgcccg cacactgatc cgaccgaggc
     7081 aggtgacggc agggccatgc acgtccgtca cctctcgctc gtcgacttcc gctcgtatgc
     7141 cgtcgcggag ctgccgctcg ctccgggtgt cacaacgatc atgggcctca acggccaggg
     7201 caagaccaac cttgtcgagg ccatcggcta tgtggcgacg ctcggcagtc accgggtggc
     7261 caccgaccag ccgatggttc gcttcggggc agcccagacg gtcatccgcg ccgcgatcgt
     7321 ccgccacgat cgggagaccc tcgtcgagct tgagttgacg ccgggccggg ccaaccgggc
     7381 tcgcctcaac cgggccccgc tcacgcggac ccgcgacgtc ctcggcaccc tgcggacggt
     7441 gctcttcgcg cccgaggacc tcgcgctggt caagggcgac ccggcgggtc gacgggcgtt
     7501 cctcgacgaa ttgctcgtcg cgcggcagcc ccgctgggcc ggggttcggg ccgactacga
     7561 acggatcctc aagcagcgca acgccttgct caagtcggcg gcgccagtgc tgcgcaaggg
     7621 ctcgtcccgg cgccggtcgg ccagcctcgc ccctggcgaa aaccctgaca cggcaacgga
     7681 atccaccctg cacaccctgg acatctggaa cacccacctt gccgaggtcg gcgcccaact
     7741 gctctatgcc cggctgcggc tgttgcgcga cctcaccccc ttcctcgcgg cggcatacgc
     7801 aacggtgagc gcgggagtca ccccggtgtc ggcgagctac cgcgcgagtc tgggtgagtc
     7861 ggtgaccgca gccatcggtg ccggtgaggt ccccgacctc gaggatctgc gagccgagtt
     7921 gctcgcggcc ttcgaggcca accgggccgc cgaagtcgag cgaggggtca ccctcgttgg
     7981 gccgcatcgc gacgacgtcc acctggcgct ggggccgatg ccggccaagg gctatgccag
     8041 ccatggcgaa tcctggtcgc tggcgctggg actcaagctc gcggcatacc aactgctgcg
     8101 gcgcgacctc ggcgaggacc cggtgctcgt cctggacgac gtgttcgccg agctggacac
     8161 ccagcgacgc gagcggctgg ccgagctcgt cgccgactgc gagcaggtgc tcgtcaccgc
     8221 cgccgtgggc gcggacgtcc ctgagcgact gcgctccacc ggccgcacgt atgccgtcac
     8281 gctcggcgag gtgacccgtg tcgactgacg aggcgggccc gctctccgta ccggactccg
     8341 acgtggaggc gggcagggac acggagccgg acactgagcc agacgctgag ggcctgccgg
     8401 gcaccgaccc ggccgaggcc tctcgcagtg cactggcccg agcgcgtaaa gccgcacgtg
     8461 acaagggttt tcgcccaggg atgaagccgg ctcccaactc ccgtcgcatc gctggtatgc
     8521 cgcccacccg cagcgccggg gacgaccgag accccgcgct tctgggtgcg cagttggagc
     8581 gactgttgct cgaccgcggc tggtccgccg acgtcaccgt gggctcggtc atgggtcggt
     8641 gggccgagat cgtcggggcg gagatcgccg ggcacgtgcg gccggtgacc ttcgagggaa
     8701 ctgtcctcag cgtccaggcc gactcgacgg catgggcgac ccagatccgg ctgctcacct
     8761 cgtcgctgct gggccggatc gaggcggtgg tgggcgctgg catcgtcacc gacctggtcg
     8821 tactcgggcc agcggcccct tcgtgggtca agggccgccg ccgcgtgcag ggccccggcc
     8881 cccgtgacac ctacggctga ccgacggggc ggccccgcag tcgaggtggc gttcgttgcc
     8941 tgacgttcca cgtgaaacct cggccccagc ggcataccag ggcctcgcct gacccggcac
     9001 tgcgccgacg gccgcctcta ggctggacga catgtcgagc cgacgcgggg tcatggtcac
     9061 cgttcccgag ttggccgccg ctttggccgg ctccgccccg ccggcgctgc tggatgtccg
     9121 gtggactctc ggcgaagtgc ctggctcggg acggcagcgg tatgccgcgg gccaccttcc
     9181 cggtgccgcc ttcc
//
LOCUS       DAOMZY010000121         9194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_7127_length_9194_cov_5.770434, whole genome shotgun sequence.
ACCESSION   DAOMZY010000121 DAOMZY010000000
VERSION     DAOMZY010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9194
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7127_length_9194_cov_5.770434"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..3839)
                     /gene="dnaB"
                     /locus_tag="PLK64_08255"
     CDS             complement(84..3839)
                     /gene="dnaB"
                     /locus_tag="PLK64_08255"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPV79777.1"
                     /translation="MSIQELFPGALDGPGDDRLPPQDVHAEQSVLGSMLLSKDAIADA
                     VEVLRGVDFYRPAHESIYEAILDLYGRGEPADAVTVADELTKRGALARVGGGAFLHQL
                     ISSVPTAANAGYYAHIVAERAVLRRLVEAGTRIVQLGYAQSGGDVEDIVNAAQAEVYS
                     VSDKRGGEDYTKLGDILELTVDEIEVASGKSGEMTGVPTGFTDLDALTNGLHPGQMIV
                     LAARPAMGKALALDTPLATPDGWTTMGEVAVGDLVMGADGRPTRVVAATEVMVDRPCY
                     EVEFSDGSVIVADAKHQWLTDTRASRRSFQAARDQRNRTKTQRTLPTVKTTEAIAATL
                     RTDTADQRLNHSVANAAALELDERELPLSPYVLGAWLGDGNSAGAALTSADAEMVMLL
                     EAEGLVVRPQTTAMRYSLTLPIDPAEVRACVVCGDEFTPKAANVVTCGRSCGGRAKGF
                     GQSAECPDCGEPSFARTRCRTCSADHGSVQALLRKVGVLGKKHVPASYLRASEPQRRA
                     LLAGLLDTDGTVTNTGSVQFTTTSPALAEGVFELLVSLGYRVGVARKQVRGRTAASST
                     AYNLNFTTDDDVFRLSRKAILHKERRTKTFNRRGSRFITAVRAVAPVPVRCIEVEAAD
                     HLYLAGRSMIPTHNSTLGLDIARSAAIKHNQAAVVFSLEMSKTEITMRLLSAEAQIQL
                     QHMRKGTMREDDWQRLAKTMGRVSDAPLFIDDSPNMSLMEIRAKCRRLKQRHNLKLVV
                     IDYLQLMSSGKRVESRQQEVSEFSRALKLLAKELEVPVIAISQLNRGPEQRTDKRPQM
                     SDLRESGCLTADTRVLRADTGAETTLGTLYAAGARDVPVWALDDTLRYVRRPMTRVFP
                     TGVRPVFRLTLESGKEIRATANHPFLTYAGWSPLGELRTGTRVAVPRHVPAPAFFEPW
                     EDPRVILLAQVLSDPAYTVDRKHANVIGDWLDSLGVLSAKPHKRRLPKALFHVSKPQI
                     ALFISHLWSLSGSASVAKSGDRGRIRMESSSRRLVEDVSLLLLRFGISTRLTETEPAG
                     GALAAYRLEVSGEDSQRRFLQEIGLHGESRKAAKRLLEIVRGGQEVGAGTAAVPPRVV
                     EAMRRVMSVSAAPRPVQGMGVAGVGAGAAGPHAGAASDRRAAGEPLREVAAVLDSADM
                     DLYAVNDVLWDPVVSIEADGVEEVFDATVLGGHNFVANGIAVHNSIEQDADMVILLHR
                     EEAYEKETQRAGEADLIVAKHRNGPTDTIVVAFQGHYSRFTNMAQQF"
     gene            4231..4515
                     /locus_tag="PLK64_08260"
     CDS             4231..4515
                     /locus_tag="PLK64_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010552172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HPV79778.1"
                     /translation="MCRNIRQLHNFAPPATSDEVYAASLQYVRKVAGSTHPSQANREA
                     FDEAVAAVAAATAHLLEHLTTTAPPKDREVEAAKARARTAERYGRPVATG"
     gene            complement(4561..5025)
                     /locus_tag="PLK64_08265"
     CDS             complement(4561..5025)
                     /locus_tag="PLK64_08265"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HPV79779.1"
                     /translation="MDPSDLMLAYGTLRAGVPPYEEFDLPARETVRAGVRVPGWLFDL
                     GAYPAARLDLAALRGERPVTATFVADVVRFGLDRPVDEALATFDEYEEVDPDSSPNIY
                     RRLLIPLAGLGGLTDDSVGVGADLAGRWAWIYEWVGSIEDLPEVPSGDWLAR"
     gene            5156..5887
                     /locus_tag="PLK64_08270"
     CDS             5156..5887
                     /locus_tag="PLK64_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HPV79780.1"
                     /translation="MTHPRRDVIDAIRAALLAHADPVRGAGQQRYMKSTLPYLGLTSP
                     TLKAALRPVLGDPAYRISTRPEWEATVRALWDEATHREHWYAALALLQHRHYRAWRDP
                     DLLPLVRHLVVSGAWWDVVDDIATHVVRDLLLGWPEPIATVLRAWSRDDHLWIRRVAM
                     LAQVGAGARTDPVLLADVLTPNIGYAGEQVFFSRKAIGWALRDYAWTAPDWVRAYVER
                     HPDLSGLSRREALKHLSQPTDAPTT"
     gene            5914..7263
                     /locus_tag="PLK64_08275"
     CDS             5914..7263
                     /locus_tag="PLK64_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPV79781.1"
                     /translation="MATAPPANPGDARVDRREIIGLAVPAFLALVAEPIFLAADSAIV
                     GHLGTIPLAGLGVAGSVLMAAAGIFVFLAYATTSVVARAMGAGSPTTAITAGLDGLWL
                     ALGLGAIAAAVVTAAADPLTAAFGASAAAQEQATTYLRIGALGLPPMLVLLAVTGALR
                     GLLDTRTPMLVAVAGFAVNIALNLAFVHGFHWGIAGSAWGTVIAQTGMALVLTAVLVR
                     KARATHAPLRPHPGRVLAAARNGIPLVVRTLALRGAILLTVWAAAVLGDAPLAAHQIA
                     ATVWSFLAFALDALAIAAQAVVGRDLGAGRVDTARAVMRTLLRWGLLFGLAVGALTAA
                     AAGLVPRLFSSDPAVIGYAVPALLVVAAGQVVAGYVFVADGVLMGAGDFRYLAWAMVV
                     ALAAYAPVVLFVRALGVWGEPGRVLVWLWGGYTAFMLARAVSLGWRLRGDRWLIPGS"
     gene            7383..8480
                     /locus_tag="PLK64_08280"
     CDS             7383..8480
                     /locus_tag="PLK64_08280"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HPV79782.1"
                     /translation="MSPLTLLAAPLVVAGVFLLSGVAKLGDVSDGVRGLREMGVPDVF
                     VRDWVARAHPIAEIIIALGLLVLPTPWRVVAGVAAVGLMTAYTVLVIAAVRRAREVNC
                     NCFGRRSEVVNRRTAVRNLALLALAIVALVDCLVPSAPIVRMFSGLDPLAWSVVLVVT
                     AGVAWLIGRGYAASAPTEPVAALSPILGGHHHEGNGESNGDDEDDEDLERDAIPDHTL
                     TAADGTTITLTELVAQRATVLLFMEPGCGPCGVLMAEVPSWRAIMPQITLTAVRLRPV
                     PGAKGLHEHAGPWQGTGYGTEPGTGEPFPTLEGGYWADPSIIQAFEIQGMTPWAVLLG
                     ADGLIAGGPVASYPHVRAFLVEILAHLAGLA"
     gene            complement(8581..9027)
                     /gene="rplI"
                     /locus_tag="PLK64_08285"
     CDS             complement(8581..9027)
                     /gene="rplI"
                     /locus_tag="PLK64_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPV79783.1"
                     /translation="MKVILTHEVSGLGTAGDVVDVKDGYARNYLFPRKLGTSWTAGGQ
                     KQVDSILKARESRTIKSLDDAKAVKSRIEGTTVTLSAKAGNSGRLFGAVSGSDLAAAV
                     VKAGGPELDKRKIEVPGHIKTVGSYVALVRLHPEVQAKLAFEVVKA"
     gene            complement(9043..>9194)
                     /gene="rpsR"
                     /locus_tag="PLK64_08290"
     CDS             complement(9043..>9194)
                     /gene="rpsR"
                     /locus_tag="PLK64_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPV79784.1"
                     /translation="ALLRKFISDRGKIRARRVTGVTVQEQRQIANAVKNAREMALLPY
                     SSSAR"
BASE COUNT         1350 a         3315 c         3073 g         1456 t
ORIGIN      
        1 gctaattcat ggaatcagcg gcacttctta cagttttgag tgcatctcga cagccttgga
       61 tcggcgctgc accgcggcat acctcagaac tgttgggcca tgttggtgaa gcggctgtag
      121 tgaccctgga aggcgaccac gatcgtgtcg gtggggccgt tacggtgctt ggccacgatc
      181 aggtcggcct cacccgcccg ctgagtttcc ttctcgtagg cctcttcgcg gtgcaacagg
      241 atcaccatgt cggcgtcctg ctcgatcgag ttgtgcaccg cgatcccgtt ggccacgaag
      301 ttgtgcccgc ccagcaccgt cgcgtcgaag acctcctcga ccccgtcggc ctcgatcgag
      361 acgaccgggt cccagaggac gtcgttgacg gcatacaggt ccatgtcggc cgaatcgagg
      421 accgcggcaa cctcgcgcaa cggctccccc gccgcccgcc ggtcactcgc ggcaccggcg
      481 tgcggccccg cagcgcccgc accaaccccg gccacgccca tcccctgaac cggccgcggc
      541 gcggcggaca cgctcatcac ccggcgcatc gcctcgacga cccgcggcgg cacggcagcg
      601 gtccccgcgc ccacctcctg cccgccgcgg acgatctcca gcagccgctt ggccgccttg
      661 cgcgactccc cgtgcagccc gatctcctgc aggaagcgcc gctgcgagtc ctcgccggac
      721 acctccagcc ggtatgccgc cagcgcacca cccgccggct cggtctcggt caggcgcgtg
      781 gagatgccaa aacgcagcag cagcagcgac acgtcctcga cgagccgacg cgacgacgac
      841 tccatccgga tccggccgcg gtcaccagac ttggcgacac tcgccgaccc cgacagcgac
      901 cacaggtgcg agatgaacag cgcaatctgc ggcttgctca cgtggaacag cgccttgggc
      961 agccgccgct tgtgcggctt cgccgacagc accccgaggc tgtcgagcca gtcaccgatg
     1021 acgttcgcgt gcttgcggtc gaccgtgtat gccgggtcgc tcagcacctg cgccaacagg
     1081 atgacccgcg ggtcctccca cggctcgaag aacgccgggg ccggcacgtg ccgaggaacc
     1141 gcgacccgcg tgccggtgcg cagctcaccc agaggtgacc acccggcata cgtgaggaag
     1201 gggtggttgg cggtggcgcg aatctccttg cccgactcca aggtgagccg gaagaccggc
     1261 cgcacccccg tcgggaagac gcgcgtcatc ggccggcgca cgtaacgcag ggtgtcgtcc
     1321 agggcccaga cggggacgtc gcgagcgccc gcggcataca gagtgccgag cgtcgtctcc
     1381 gccccggtgt ccgcgcgcag cacgcgggtg tccgcggtca ggcagccgga ctcacgcagg
     1441 tcggacatct gcgggcgctt gtcggtgcgc tgttccggtc ctcggttgag ctgggagatg
     1501 gcgatgacag gcacttcgag ctccttcgca agcagcttga gtgccctgga gaactcggac
     1561 acctcctgtt gacggctctc cacccgcttt cctgacgaca tcagttgtaa atagtcgata
     1621 acgacgagct tgaggttgtg gcgctgcttg agccggcggc acttggcccg gatctccatg
     1681 agcgacatgt tggggctgtc gtcgatgaac agcggggcgt cggacacccg ccccatcgtc
     1741 ttggccaggc gctgccagtc gtcctcacgc atggtgccct tgcgcatgtg ctgcagctgg
     1801 atctgagcct cggccgagag cagacgcatg gtgatctcgg tcttgctcat ttcgaggctg
     1861 aacacgaccg cggcctggtt gtgcttgatc gccgccgacc gcgcgatgtc cagcccgagc
     1921 gtggagttgt gcgtggggat catcgagcgc ccggcgaggt agaggtggtc cgccgcctcc
     1981 acctcgatgc accgaaccgg caccggcgcc accgcccgca ccgccgtgat gaaccgcgag
     2041 cctcgacggt tgaaggtctt ggtgcggcgc tccttatgga ggatcgcctt gcggctcagg
     2101 cggaagacat cgtcgtcggt cgtgaagttg aggttgtatg ccgtggagct ggctgccgtg
     2161 cgtccgcgga cctgcttgcg agccacaccc acgcggtaac ccagcgacac cagcagttcg
     2221 aagactccct cagcgagcgc agggctcgtc gtggtgaact gcacgctgcc ggtgttggtg
     2281 accgtgccat ccgtgtcgag cagtcctgca agcagcgccc gacgctgagg ctcactcgcc
     2341 cgcaggtaac tggctggaac gtgcttcttg cccagcaccc cgaccttgcg cagcagggcc
     2401 tgcaccgagc cgtggtctgc agagcatgtc cggcaccgag tgcgcgcgaa agagggctcg
     2461 ccgcagtcag ggcactccgc agactgacca aagcccttcg cacgcccgcc gcacgaacgg
     2521 ccacaggtaa caacgttggc ggccttgggc gtgaactcgt cgccacacac cacgcaggcc
     2581 cgcacttcag ctgggtcaat aggcagggtc aacgagtaac gcatcgctgt ggtttggggt
     2641 cgcacgacga ggccctcggc ctcgagcagc atgaccatct cggcgtcggc gctggtgagg
     2701 gcggccccag cgctgttgcc atcccccaac caggcaccca gaacataggg cgacaggggc
     2761 agttcccgct cgtccaactc cagggctgcg gcgttcgcga ccgagtggtt cagtcgttgg
     2821 tcggccgtgt cggtacgcag ggtcgcagcg atggcttcag tcgtcttgac ggttgggagg
     2881 gtgcgctggg tcttggtgcg gttgcgctga tcccttgcgg cctggaaaga ccgacggctg
     2941 gcccgggtgt cagtgagcca ctggtgcttg gcgtcggcga cgatgaccga gccgtcggaa
     3001 aactcgacct catagcaggg gcgatccacc atgacctcgg tcgcggccac gacacgggtg
     3061 gggcggccgt cggcacccat gacaaggtca ccgaccgcga cctcacccat cgtcgtccac
     3121 ccgtcagggg tggccagcgg ggtgtcgagg gcgagcgcct ttcccatcgc cggtctcgcc
     3181 gcgaggacga tcatctggcc ggggtgcagg ccgttggtga gggcgtcgag gtcggtgaag
     3241 ccggtcggca cgccggtcat ctcgccggac ttgccggacg cgacctcgat ctcgtcgacg
     3301 gtgagctcga ggatgtcgcc gagcttggtg tagtcctcgc ccccgcgctt gtccgagacg
     3361 ctgtagacct cggcctgggc cgcgttgacg atgtcttcga cgtcgccgcc gctttgggca
     3421 tagccgagtt ggacgatccg ggtgccggcc tcgaccaggc gccgcagcac ggcccgctcg
     3481 gcgacgatgt gcgcgtagta gccggcgttg gcggccgtcg gcaccgagga gatgagttgg
     3541 tgcaggaacg ccccgccgcc gacccgcgcg agcgccccgc gcttggtcag ctcgtcggca
     3601 acggtgaccg catcggccgg ctcgccgcgg ccatagaggt cgaggatcgc ctcatagatc
     3661 gactcgtggg ccggccgata gaagtcgacg ccgcgcagca cctcgaccgc gtcggcgatg
     3721 gcgtccttgg acagcagcat cgagcccagc accgactgct cggcgtggac atcttgtgga
     3781 ggaagccgat catccccagg cccatcgagg gcaccgggaa acaactcctg gatcgacact
     3841 gcttcccctg acctgccttc cttggtttgc tcacgaccac cgactctagg aaagcgcgca
     3901 gcactgacag gccttctgac ctgcgcaaac gtgctgacga caccatccgc ccccggtggt
     3961 tctcctaccc taggtccgcg cccgcccacc ctcaaccgca ccctgtgtat gccgcgtgga
     4021 cagcctgtgg aacacgccga taccccgtgt ggataaccct ggggataacc ggcgcgggct
     4081 gtggacagtc gggggacggc ataccccggc gcggggcgac cgtgcgggaa acggcgggtg
     4141 tcgcgcgtcc accgatgtgg agaacagaaa atcccgggcg tgtccctggg ggcgatgcgc
     4201 ggcgggcgga ggcggcatac cctcgtgccc atgtgccgca acatccgcca gctccacaac
     4261 ttcgcccctc ccgcgacgag cgacgaggtg tatgccgcgt cgctgcagta tgtgcgcaag
     4321 gtcgccgggt ccacccaccc gagtcaagcc aatcgcgagg cgttcgatga ggcggtggcg
     4381 gccgtggctg cggcgaccgc gcacctgctc gagcacctga cgacgacggc gccgccgaag
     4441 gaccgtgagg tcgaggcggc caaggctcgc gctcgcaccg ccgagcgcta cggccgaccg
     4501 gtcgccaccg gctgagcttt ccatgggctg agctcctcgc cggtcagcga tcggcgaggg
     4561 tcagcgggcg agccagtcgc cgctgggcac ctcgggcagg tcctcgatcg agccgaccca
     4621 ctcatagatc caggcccacc gcccggccag atcggcgcca accccgaccg agtcgtcggt
     4681 caacccgccg agcccggcca gcgggatcag cagccggcga tagatgttgg gcgaggagtc
     4741 cgggtcgacc tcctcatact catcgaacgt cgccaaggcc tcgtccaccg gccggtcgag
     4801 cccgaagcgc accacgtcgg ccacgaacgt cgccgtcacc ggccgctcac cgcgcagcgc
     4861 cgcgaggtcc agccgcgccg ccggataggc acccaggtcg aacagccacc ccggcacccg
     4921 caccccggca cgcaccgtct cgcgggccgg caagtcgaac tcctcatagg gcggcacccc
     4981 cgcccgcagc gtcccgtagg ccagcatgag gtcgctcggg tccatgatgg gggccgcgat
     5041 ccctaccggg tcgatggggc caacccggtc ggcaggatcg acagactcgg caggctcaag
     5101 aggttcaact gactcaactg gcgggcgcac cgcagcagac tagaggaggc cgcccgtgac
     5161 ccacccccgc cgcgacgtca ttgacgcgat ccgcgccgcc ctcctcgcgc acgccgaccc
     5221 ggtccgcggc gccggccagc agcgctacat gaagtcgacc ctcccctacc tcggcctcac
     5281 ctcccccacg ctcaaggcgg cgctgcgccc ggtgctgggc gacccggcat accggatcag
     5341 cacgcggccc gagtgggagg cgaccgtccg cgcgctctgg gacgaggcga cccaccgcga
     5401 gcactggtat gccgcgctcg ccctcctgca gcaccggcac taccgcgcgt ggcgcgatcc
     5461 cgacctgctg ccgctggtgc gtcacctcgt tgtcagtggt gcgtggtggg atgtcgtcga
     5521 cgacatcgcg acccacgtcg tgcgtgacct gttgctcggc tggccggagc cgatcgccac
     5581 ggtcctgcgc gcgtggtccc gcgacgatca cctgtggatc cgtcgcgtcg cgatgctcgc
     5641 ccaggtcggt gcgggcgctc gcaccgatcc cgtgctgctc gccgacgtcc tcacgcccaa
     5701 catcgggtat gccggtgagc aggtcttctt ctcccgcaag gcgatcggct gggcgctgcg
     5761 cgactacgcc tggacggcgc ccgactgggt gcgggcttac gtcgaacgcc atcccgacct
     5821 gtccgggctg tcccgccgcg aagcgctcaa gcacctctcg caacccacgg acgccccgac
     5881 cacctgacgc atccagccgc taaggtcacc gacgtggcca cggcaccgcc tgcgaacccc
     5941 ggcgacgcac gagtcgaccg gcgggagatc atcgggctgg ccgtccctgc cttcctcgcc
     6001 cttgtcgctg agccgatctt cctggccgcc gactcggcga tcgtcggaca cctcggcacc
     6061 attccgttgg ccggccttgg ggtcgcgggg tcggtcctca tggccgccgc cggcatcttc
     6121 gtcttcctcg cctacgccac gacgagcgtc gtcgcccgag ccatgggcgc cggctccccc
     6181 accactgcca tcacggccgg gctcgacggg ctctggctcg ccctcggtct cggcgcgatc
     6241 gcggccgccg tcgttactgc cgcggccgac cccctcacgg ccgcgttcgg tgcctccgcc
     6301 gcagcccagg agcaagccac cacctacctg cggatcggcg ccctcggcct ccccccgatg
     6361 ctggtcctgc tcgccgtcac gggtgctctg cgcggactgc tcgacacccg aacccccatg
     6421 ctcgtcgccg tcgccggatt cgccgtcaac atcgccctca acctggcctt cgtccacgga
     6481 ttccattggg gcatcgccgg atccgcgtgg ggcacggtca tcgcccagac cggcatggcg
     6541 ctcgtcctca ccgccgtcct agtgcgcaag gcccgagcca cccacgcccc cttgcgcccc
     6601 catccggggc gggtcctcgc ggccgcgcgc aacggcatac cccttgtcgt gcggaccctc
     6661 gcgctgcggg gagcgatcct gctgaccgtg tgggcggcgg ccgtcctcgg tgacgcgccg
     6721 ctggccgcgc accagatagc cgcgacggtg tggagcttcc tcgcgttcgc gcttgacgcc
     6781 ctcgcgatcg ccgcgcaggc cgtcgtcggg cgcgacctcg gcgccgggcg cgtcgacacg
     6841 gcgcgcgcgg tgatgcggac cctgctgcgc tgggggctgc tcttcggcct cgccgtcggc
     6901 gcgctcacgg ctgccgcggc gggcctggtg ccgcgtctgt tctcctccga ccccgccgtc
     6961 atcgggtatg ccgtccccgc cctcctcgtc gtcgccgccg gtcaggtggt ggctggctac
     7021 gtcttcgtcg ccgacggggt gttgatgggt gccggggact tccgctatct cgcgtgggcc
     7081 atggtcgtgg cgctggcggc atacgcgccg gtcgtgttgt tcgtgcgcgc gctgggcgtg
     7141 tggggcgagc cggggcgggt gcttgtgtgg ttgtggggcg gttacacggc gttcatgctg
     7201 gcgcgcgcgg tgagcctggg ttggcgactg cgcggtgacc gctggctcat ccccggctcc
     7261 tgacccctgc ccgacccccc aagctgatcc cctgccgaat cgaggtattc cgccatcccc
     7321 cgatgatggc gaaatacctc gactgggcag agggagccag ggggcgcgga tagggtgacc
     7381 gagtgtcccc gttgaccttg ctcgccgccc cgctcgtcgt cgccggcgtg ttcctcctca
     7441 gtggggttgc caagctcggc gacgtcagcg acggggtgcg cgggctgcgc gagatgggcg
     7501 ttcccgacgt cttcgtgcgt gactgggtcg ctcgcgcgca tccgatcgcc gagatcatca
     7561 tcgccctcgg gctgctcgtc ctgccgacgc cctggcgcgt ggtggccggg gtggccgcag
     7621 tcgggctgat gacggcatac accgtgctgg tgatcgccgc ggtccggcgg gcgcgcgagg
     7681 tgaactgcaa ctgtttcggc cggcgaagtg aggtcgtcaa ccgccgcacc gccgtgcgca
     7741 acctcgctct cttggcgctc gcgatcgtcg cgctcgtcga ctgcctcgtg ccgtcggcac
     7801 ccatcgtgcg gatgttcagc ggacttgacc ccctggcgtg gagtgtcgtc ctcgtcgtca
     7861 ccgccggggt cgcctggctc atcgggcgag ggtatgccgc gagcgcaccg accgaacccg
     7921 tcgccgccct gagccccatc ctcggcggac accaccacga gggcaacggc gaaagcaacg
     7981 gcgacgacga ggacgacgag gacctcgaac gcgacgcgat ccccgaccac accctcaccg
     8041 cagccgacgg aacgacgatc acgctcacgg agctcgtcgc tcagcgcgcg acggtcttgc
     8101 tgtttatgga gccggggtgc gggccgtgcg gcgtcttgat ggccgaggtg ccgagctggc
     8161 gcgccatcat gccccagatc accctcaccg ccgtccgcct cagacccgta cccggtgcca
     8221 agggtctcca cgagcacgcc gggccctggc aggggaccgg atacgggacc gagcccggca
     8281 ccggcgagcc cttcccgacg ctcgagggcg gctactgggc cgatccgtcg atcatccaag
     8341 cgttcgagat ccagggcatg acgccgtggg cggtgctgct tggcgccgac gggctgatcg
     8401 cgggaggacc ggtggccagc tacccccacg tgcgcgcgtt cctcgtcgag atcctggccc
     8461 acctcgccgg cttggcttga cctctagccg agcccccaac tcaaaaccgg atcgaggtat
     8521 tcagccatca ggagacgatg gccgaatacc tcgatccggc agagaaactg gcagaagaac
     8581 tcaggccttg acgacctcga aagcgagctt ggcctgcacc tcggggtgca gccgcaccag
     8641 cgcgacatac gagccgaccg tcttgatgtg gccgggaacc tcgatcttgc gcttgtcgag
     8701 ctccgggcca ccggccttga cgaccgcggc agccaggtcc gacccgctca ccgcgccgaa
     8761 caggcgacca gagttgccgg ccttcgccga cagcgtgacg gtcgtgccct cgatgcggga
     8821 cttgacggcc ttggcgtcgt cgagcgactt gatggtgcgc gactcacgag ccttgaggat
     8881 cgagtcgacc tgcttctggc caccggcggt ccagctggtg cccagcttgc gggggaacag
     8941 gtagttgcgg gcgtaaccgt ccttgacgtc gacgacgtcg ccggcggtgc cgagcccgct
     9001 cacttcgtgg gtgaggatga ccttcatctg tggctcctta ggtcagcggg ccgacgacga
     9061 gtagggcagc agcgccatct cgcgggcgtt cttcacggcg ttggcgatct gccgctgctc
     9121 ctggacggtg acaccggtga cccggcgagc gcggatcttg ccgcggtcgg agatgaactt
     9181 gcgcagcagc gcag
//
LOCUS       DAOMZY010000122         9116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_7205_length_9116_cov_5.245116, whole genome shotgun sequence.
ACCESSION   DAOMZY010000122 DAOMZY010000000
VERSION     DAOMZY010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9116
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7205_length_9116_cov_5.245116"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /gene="narY"
                     /locus_tag="PLK64_08295"
     CDS             <1..788
                     /gene="narY"
                     /locus_tag="PLK64_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146608.1"
                     /note="with NarZV catalyzes the reduction of nitrate; the
                     beta subunit is an iron sulfur cluster containing electron
                     transfer subunit; one of 3 nitrate reductases in E. coli;
                     expression of nitrate reductase Z is not dependent on
                     nitrate levels; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HPV79785.1"
                     /translation="ACFLDPSDPQVQQAARAAGIPQEWVRAAERSPVWKLISRYEVAL
                     PLHPEYRTMPMVWYIPPLSPVVDVIRDSGHDAEDADNLFAAIDTLRIPVEYLANLFTA
                     GDTGPVDRALRRLAAMRSYMRERTMGNEPGPAMAQAVGMSEEDLYEMFRLLAIAKYAD
                     RYVIPTAHAEQAHALEELATECALDSDGGVGMGGSGPVGEGSGRLTPVAVENLRVLKA
                     RAAAGTSGEELVGGGRKSGRVNLLSWDGKGIPEGLFPPDRKDG"
     gene            788..1477
                     /gene="narJ"
                     /locus_tag="PLK64_08300"
     CDS             788..1477
                     /gene="narJ"
                     /locus_tag="PLK64_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007270347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HPV79786.1"
                     /translation="MFRRSRHTAAPTRLPDDQLSIAWQVCSLLLDYPSEALLARLELA
                     RRAAGELPAYLAEPVCRVVDHLESTPLGQVQRDFVETFDHTRRGCLYLTYFTTGDTRK
                     RGVALVRLKQAYRRAGVELTSGELPDHLAVVLEFGATADIEAAWRILNDYRASIEVLR
                     ISLQEKNSPWADAVVAISRTLPELAGDDAEAIAKLIEQGPPSEDVGLAPYAVDPRLNP
                     LPDYLAVGADR"
     gene            1474..2214
                     /gene="narI"
                     /locus_tag="PLK64_08305"
     CDS             1474..2214
                     /gene="narI"
                     /locus_tag="PLK64_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HPV79787.1"
                     /translation="MTTFLWVIFPYLCLTTFVVGHYWRYRYDKFGWTTRSSQLYEDRL
                     LRIGSPLFHFGMLGVLGGHIIGLVIPQGWTDALGLSRHTYHVIALAAGIPAGIAAVVG
                     LCILVYRRRTTGPVFSATTVNDKVMYAVLGAVIALGIWNTIAGSWLQFGGDYNYRDGV
                     SPYFRSIFGFQPKAALMAAAPLGFQAHALLAFVLFAMWPFTRLVHVFSAPLGYLTRPY
                     IVYRSRDPQSEQGLGSRAPKRGWERVGS"
     gene            2211..2840
                     /locus_tag="PLK64_08310"
     CDS             2211..2840
                     /locus_tag="PLK64_08310"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HPV79788.1"
                     /translation="MTGSPDGFAGHPAFDAIILAGGRGSRLGGVNKGAVRISGRTLLD
                     RALDAVGSAARVVVVGDGPVPEGVLSAREEPAFGGPAAAVVAGLHALRMPSRAPRVLV
                     LACDVPGAAEGVGLLMAACAGEEGPSGSAVGRPEVDGWCLAEPDGRLQWLFGLYRVPA
                     LERAAEALGDPSDRSMGRLLGDLHLTAVPAPWRATADIDTPEDLQRWTS"
     gene            2828..3181
                     /locus_tag="PLK64_08315"
     CDS             2828..3181
                     /locus_tag="PLK64_08315"
                     /inference="COORDINATES: protein motif:HMM:NF040429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6457 domain-containing protein"
                     /protein_id="HPV79789.1"
                     /translation="MDIVRTERTAHDLAAEQAQWQTWIDAVCADLGVPSSLVDVEAIH
                     DLSRQVAHGLARPLAPVSTFILGLALGRALGSSGAAVSGSADGSAESDGSGSSDGRTT
                     AEHRAALSDRIAAHI"
     gene            complement(3466..4581)
                     /gene="ald"
                     /locus_tag="PLK64_08320"
     CDS             complement(3466..4581)
                     /gene="ald"
                     /locus_tag="PLK64_08320"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HPV79790.1"
                     /translation="MKVGIPAEVKNHEYRVAITPVGVHELVGHGHEVMIQKGAGLGSS
                     ITDDDYISQGARIIDSADDVWGEADMVLKVKEPVAEEYHRLREGLTLFTYLHLAADKP
                     LTEELMAKKVTGIAYETVQLPSGMLPLLYPMSEVAGCLAPQVGAHAMMRAQGGRGVLM
                     GGVGGVANAKVVVLGAGVAGQNAANIALGMGADVTLLDTDLDKLRMSFWRYDNRVHQI
                     ASSKLSVREQVLGADLVIGTVLIPGAKAPKLVTDDMVAAMKPGSVLVDVAIDQGGCFE
                     GSRPTTHADPTFAVHDAVYYCVANMPGAVPNTSTWALTNATLSYAVKLADKGWKGAIG
                     ADQALALGLNTHDGFVTYKAVADDLDLPYRSLADVLA"
     gene            4766..5254
                     /locus_tag="PLK64_08325"
     CDS             4766..5254
                     /locus_tag="PLK64_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HPV79791.1"
                     /translation="MPFEAEPTAYSGLDGLDRTILDALQEDGRLTNVDLARRVGLSPS
                     PCLRRVKSLEERGYIQGYAALLDPALLGRALQVVVMVRLTDQRQQTLAAFESAVEGIE
                     DVLRCSLIAGDADYLLTVQVRDLDSYHALYTKRLGELPGVASLRSLVIMKAVKDTHAL
                     PV"
     gene            complement(5282..5719)
                     /locus_tag="PLK64_08330"
     CDS             complement(5282..5719)
                     /locus_tag="PLK64_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPV79792.1"
                     /translation="MIALDTNLLVYAHREDSPHHAAAAAALQSAITAPGGCAIPWPCL
                     HEFLAVVTHPRIYDPPTPMGTAVAAVRALVDAPSVHLLGEGVSHLDRLAALLASGAVV
                     GPRIHDARVAAICLDHGVDALWSADRDFSWFPRLTVVNPLVAG"
     gene            complement(5716..5967)
                     /locus_tag="PLK64_08335"
     CDS             complement(5716..5967)
                     /locus_tag="PLK64_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79793.1"
                     /translation="MKTTIEIPDALAEDARQVARAGGTTLRDLVVTGLMAEVERRRNA
                     PRVEFTFPTVGGRGLVVDLAPGEVIDRSYGFASGPGSSR"
     gene            6155..7402
                     /locus_tag="PLK64_08340"
     CDS             6155..7402
                     /locus_tag="PLK64_08340"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HPV79794.1"
                     /translation="MTLLAVGVSHHHLGSDHLAELEVLAPDLTRAIESSTAVEGLVVV
                     ATCNRFEAYLDATAFHPAVEAVVAALRATPLADPQAIIEALDVHPGESAVEHLFSVAC
                     GLESMVVGEAQVVGQVRQALAEADAAKRATPALHRLFQDALATAKTVASTTELGGRGR
                     SIASVGLDLLEAQHSPVAGSQALVLGTGSYAGVVVAELTRRGCTDIAVYSRTGRADSF
                     ARTHPVTPVPEPGLLDALARADLVVTCSGASSPTLTDALVKAQRRYAARVLPILDLST
                     GGELGDGVAELDEADVFTLDEIGRSAPAEHVDTLLAAQDVVKRSVAAYLHVEGGRTAA
                     PAITAMRAYVSAIIAREAEVAARRYPPEVAEAIDRSLRRVSGALLHAPSVRAAELART
                     GDLEDYTHAMATLFGIVVEADSW"
     gene            7396..8283
                     /gene="hemC"
                     /locus_tag="PLK64_08345"
     CDS             7396..8283
                     /gene="hemC"
                     /locus_tag="PLK64_08345"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HPV79795.1"
                     /translation="MVRLGTRGSLLARTQSTQVAEALYAATGLRAELVIIRSEGDDHR
                     IPLHAPSRPGIFVATLRDALLAGKVDLVVHSFKDLPSAPMPGLVIPAIPARRSPADVL
                     VTATGGGLADLAVGARVGTSSPRRAAALLRVRPDLTIVPIRGNVDTRVAAVAAGTVDA
                     VVLAQAGLERLGHLTDRMTPLPTQVMLPAPAQGALAVECRAADPLADHLAVLDDPRAR
                     LEVTAERAVLTGVEATCTTAIGALARWESGALTLRAELTEDGTRSHTHAEHSRTLANE
                     HDLDGAAALGAQVAEWLLL"
     gene            8268..9077
                     /locus_tag="PLK64_08350"
     CDS             8268..9077
                     /locus_tag="PLK64_08350"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HPV79796.1"
                     /translation="MAALVTATPTGADLLPGPVVLVRPDRNEADAAALRDAGFTVLVE
                     PYLSVTTADDPAPALALLDVLDPRRTGRDTWLVLTSARTMAHWAALVGPHLLYAALDA
                     AHEGGLRVACVGSATAATLPDGMSPNLTATNSDASALLDDLLAATAYPMLALLPGSAI
                     ARPTLADGLTAAGWAVRTAAVYDTRSVLTRPASADLLAAGTAAALVLRSPSAVAAVAG
                     FVQPNPSVTIVAVGPTTTAAVREQGWSAITPASPDSGAVARALSRALTRSQ"
BASE COUNT         1307 a         3320 c         3051 g         1438 t
ORIGIN      
        1 gcgcctgctt cctcgacccc agcgacccgc aggtccagca ggcggcccgc gccgccggga
       61 tcccgcagga gtgggtccgg gcggccgaac gctcgcccgt ctggaagttg atcagccgct
      121 atgaagtggc cctgccgctg cacccggaat accgcacgat gccgatggtt tggtacatcc
      181 cgccgctctc cccggtggtc gatgtcatca gggactccgg gcacgacgcc gaagatgccg
      241 acaacctctt cgcggcgatc gacaccctgc ggatccccgt tgagtacctc gcgaacctct
      301 tcaccgccgg ggacaccggt ccggtcgaca gggcactgcg tcgcctggca gccatgcggt
      361 cctacatgcg cgagcggacc atgggcaacg agcccggccc agcgatggcc caggccgtgg
      421 ggatgtcgga ggaagacctc tacgagatgt tccgactgct ggcgatcgcg aagtacgccg
      481 accgctatgt catcccgacc gctcacgccg aacaggccca cgccctcgag gaattggcca
      541 ccgagtgcgc gctcgacagc gacggtggcg tgggaatggg tggcagcggg ccggtcggcg
      601 aaggttccgg ccgcctcacc cctgtggccg tcgagaacct ccgagtgctc aaggcgcgcg
      661 cggccgccgg cacctccggc gaagagttgg tcggcggcgg gcgcaagagc ggccgggtca
      721 acctgctctc gtgggacggc aagggcatcc ctgaaggcct cttcccaccc gaccggaagg
      781 acggctgatg ttccggcgct cacggcatac ggccgctccc acccgactgc ccgacgacca
      841 gttgagcatc gcgtggcagg tctgctcgtt gttgctcgac tacccgagcg aggcgctcct
      901 cgcgcggttg gaactcgcgc ggcgagcggc cggcgagttg ccggcatacc tcgcggagcc
      961 ggtatgccgc gtggtggacc acctggaaag cacgcccttg gggcaggtgc agcgtgactt
     1021 cgtggagacc ttcgaccaca cccgtcgcgg ctgcctctac ctcacctact tcaccaccgg
     1081 cgacacccgc aagcgtgggg tggccctcgt gcggctcaag caggcctacc gccgggcggg
     1141 tgtggagttg accagtggcg agttgcccga ccacctggcg gtcgtgctcg agttcggcgc
     1201 gacggccgac atcgaggcgg cctggcggat cctcaacgac tatcgcgcga gcattgaggt
     1261 gctgcgaatc tcgttgcagg agaagaactc tccatgggct gacgccgtcg tcgccatcag
     1321 ccggacgctc cccgaactcg ccggtgacga cgccgaggcg atcgccaaac tcatcgagca
     1381 agggcccccc tcggaggacg tcggcctggc gccctacgcc gtggacccgc ggctcaatcc
     1441 cctgcccgac tatctcgccg ttggagccga ccgatgacga ccttcctgtg ggtcatcttc
     1501 ccctacctgt gtctcacgac cttcgtcgtc gggcactact ggcgctaccg ctacgacaag
     1561 ttcggctgga ccacccgctc cagccagctc tatgaagacc gactgctgcg gatcggctcg
     1621 ccgctcttcc atttcgggat gttgggggtg ctcggcggcc acatcatcgg tctggtgatc
     1681 cctcaggggt ggaccgatgc gctcgggctg agccggcata cctatcacgt catcgcgctg
     1741 gccgcgggca tccccgcggg catcgccgcc gtggtcggtc tgtgcatcct cgtctaccgc
     1801 cgacggacga ccggtccggt cttcagcgcg acgacggtca acgacaaggt catgtatgcc
     1861 gtcctcggcg cggtcatcgc gttgggcatc tggaacacca tcgccgggtc gtggctgcag
     1921 ttcggcggcg actacaacta ccgcgacggg gtgtcgccct acttccgcag catctttggc
     1981 ttccagccca aggctgcgct catggcggcg gcccccttgg gcttccaggc ccacgcgctg
     2041 ctcgcgttcg tgctcttcgc gatgtggccg ttcacccggc tcgtccacgt cttctccgcg
     2101 ccgctgggct acctcacccg tccctacatc gtctatcgct cacgtgatcc gcagtctgag
     2161 caaggcctgg gcagccgggc accgaagcgg ggttgggaac gagtgggctc gtgaccggta
     2221 gccctgacgg gttcgccggc cacccggcat tcgacgcgat catcctcgcg ggcggacgtg
     2281 gctcgcggct cggcggggtc aacaaggggg ccgtgcgcat ctctggtcgg accctgctcg
     2341 accgcgcact cgacgcggtc ggctcggccg cgcgcgtcgt cgtggtcggc gatggtcccg
     2401 tccccgaggg tgtgttgagt gcgcgcgaag agcctgcctt cggggggccg gccgccgccg
     2461 tggtcgccgg gctgcacgcc ttgcgtatgc cgtcccgcgc acctcgcgtg ctcgtcctgg
     2521 catgcgacgt gcctggcgca gcagagggcg ttgggctgtt gatggctgcg tgtgccggcg
     2581 aggagggtcc gtcggggagt gcggttggtc ggcccgaggt cgacggctgg tgcctggccg
     2641 agcccgatgg ccggctgcag tggctcttcg ggctctatcg cgtgcctgca ttggagcgag
     2701 cggccgaggc gctcggcgac ccgagcgacc ggtcgatggg tcggctgctc ggcgacttgc
     2761 acttgaccgc tgttcctgcc ccgtggcggg ccaccgccga catcgacacc ccggaggact
     2821 tgcaacgatg gacatcgtga ggaccgaacg cacggcacat gatctggccg ccgagcaggc
     2881 acagtggcag acctggatcg acgccgtgtg tgccgacctg ggggtcccct cgtcactcgt
     2941 ggacgtcgag gcgatccacg acctgtctcg acaggtcgcc cacgggctcg ctcgaccgct
     3001 ggcgcccgtg agcaccttca tcctcggcct ggcgctcggt cgagcactgg gctcgtccgg
     3061 ggctgccgtg tcagggtcgg ccgatgggtc cgctgagtcc gatgggtcag ggtcgtccga
     3121 tgggcgaacg accgctgagc accgggcagc actctcggat cggatcgccg cacacatctg
     3181 accccagaaa caccgagcga cctacctgcc tgtcacaaac caccggcggg tgtgccgaaa
     3241 tcgggaccac ccggcatacc cctcgcccag atcaccggcg ggtgtgggga tatcggggca
     3301 atcaaggccc gctagccaac aagacgtcgg cgggtgtgag gatcttggga ccagcgtggt
     3361 cctgaggcgg aggaacagcg agccccgggg cctgacggat caggctcccg gggctcggtc
     3421 gtccacacgg ttcggattgg ggtgggagag caccccccgt ccgaatcagg ccaggacgtc
     3481 tgccaaggag cggtaaggca ggtcgaggtc gtcggccacg gccttgtagg tgacgaaacc
     3541 gtcgtgagtg ttcaggccga gggccagggc ctggtcggcg ccgatggcac ccttccaacc
     3601 cttgtcggcg agcttcacgg catacgagag ggtcgcgttg gtgagcgccc aggtggaggt
     3661 gttcgggaca gccccaggca tgttcgcgac gcagtagtag acggcgtcgt ggaccgcgaa
     3721 ggtcgggtcg gcgtgcgtcg tcgggcggct gccctcgaag catccaccct ggtcgatggc
     3781 gacgtcgacg aggaccgagc cgggcttcat cgcggcaacc atgtcgtcgg tgacgagttt
     3841 gggggccttg gcgccgggga tgaggacggt gccgatgacg aggtcggcgc cgagcacctg
     3901 ctcacgcacg gacagcttcg aggaggcgat ctggtggacg cggttgtcgt agcgccagaa
     3961 gctcatccgc aacttgtcga ggtcggtgtc gagcagcgtg acatcggctc ccatgccgag
     4021 ggcgatgttg gccgcgttct gaccggcgac gcccgcgccg aggacgacga ccttggcgtt
     4081 ggcgacgccg ccgacgcctc ccatgaggac accgcggcca ccctgggccc gcatcatcgc
     4141 gtgcgcgccg acctgggggg ccagacagcc ggcgacctca gacatcgggt agagcagcgg
     4201 gagcatcccg gagggcaact ggaccgtctc gtaggcgatg ccggtgacct tcttggccat
     4261 cagctcctcg gtgagcggct tgtcggcggc caggtgcagg taggtgaaga gggtcagtcc
     4321 ctcgcgcagc cggtggtact cctcggcgac gggctccttg accttgagga ccatgtcggc
     4381 ctcgccccac acgtcgtcgg cggagtcgat gatccgtgcg ccctggctga tgtagtcgtc
     4441 gtcagtgatc gaggagccga gcccggcgcc cttctggatc atgacctcgt gcccgtggcc
     4501 gacgagctcg tggacgccga ccggggtgat ggcgacccgg tattcgtggt tcttgacctc
     4561 ggcggggatc ccaaccttca tgtcgtcacc atttctcgac cccacggggc cgctgtcctg
     4621 tccgaggccc cgcagggccg gccgcgtcgt cgcgggctcg tcggcaattc tagacgcggg
     4681 gctctggcat gtggtgccct tctacacccc gctctgccct attcttggca tgttctgctt
     4741 ctcataccga ctctaggagt gactcgtgcc cttcgaagct gagcccacgg catactcggg
     4801 cttggacgga ctggaccgaa cgatcctcga cgcgctgcaa gaggacggcc gactcaccaa
     4861 cgttgacctc gcgcgtcggg tggggttgtc cccctcgccc tgcctgcggc gggtcaagtc
     4921 actggaggag cgcggctaca tccaggggta tgccgcgctg ctggaccccg cgctgctcgg
     4981 ccgggcgctg caggtcgtcg tcatggtgcg ccttaccgat caacggcagc agaccctggc
     5041 ggcgttcgag tcggccgtcg agggcatcga ggacgtcctg cgctgctccc tcatcgccgg
     5101 cgacgccgac tatctgctga ccgtgcaggt gcgcgacctc gactcgtatc acgcgctcta
     5161 caccaagcgg ctcggcgaac tccccggagt ggcgtcgctg cgcagcctcg tcatcatgaa
     5221 ggcggtcaag gacacccacg ccctcccggt gtgaaggtcg ccgccctcgc actccggcca
     5281 ctcagccggc caccaaggga ttcaccaccg tgagcctcgg gaaccaggag aaatcacggt
     5341 cggctgacca cagggcatcg acgccatggt caaggcagat cgcggcaacc cgggcgtcgt
     5401 ggatgcgagg accgacgacc gcgcccgaag ccaacagggc ggcaagtcgg tcgaggtgac
     5461 tcactccctc gcccagcaga tgaaccgacg gagcatcgac cagggcccgg acggctgcca
     5521 ctgcggtgcc cattggcgtc ggagggtcat agatccgcgg gtgggtgacg acagcgagga
     5581 actcgtgcag acacggccag gggatcgcac acccgccggg ggcggtgatc gccgactgga
     5641 gtgccgcggc cgctgccgcg tggtgcggcg agtcctcgcg gtgggcgtag accagcagat
     5701 tcgtgtcgag agcgatcacc tcgatgagcc cgggcctgag gcgaatccgt aggaccgatc
     5761 gatgacctcg cccggcgcga ggtcgaccac caggccacgc cccccgacgg tggggaaggt
     5821 gaactcgacc cgtggcgcgt tcctgcgtcg ctcgacctcc gccatcaggc cggtgacgac
     5881 caagtcccgc agcgtcgtcc ccccagctcg cgccacttgc ctcgcgtctt cggcgagagc
     5941 atccgggatc tcgatcgtcg tcttcatacg gcttaccgta cttcatcacc cgtatacccg
     6001 tactcgtggt gccgccccga cgagccgccg gaggaccacc attcccgcct cggcccacac
     6061 ccggttgtcc aagccggata acgccttccc accagccgtt tttcgacact ggcggtggta
     6121 aaagcccacc ccgcgcgtgg ttaccatcga atccgtgacc ttgctcgccg tgggtgtcag
     6181 ccaccaccac ctcggctcgg accatctcgc cgagctcgag gtgctggcgc ccgatctcac
     6241 ccgagccatc gagtcttcca ctgcggtcga gggcctcgtc gtggtcgcca cgtgcaatcg
     6301 gttcgaggcg tatctcgatg ccaccgcgtt ccatccggcc gtcgaggccg tggtcgccgc
     6361 cctgcgcgcg accccactgg ccgacccgca ggcgatcatc gaagccctgg acgtccaccc
     6421 cggcgagagc gccgtcgagc acctcttctc ggtcgcttgc ggcctggagt cgatggtcgt
     6481 cggcgaggcc caggtcgtcg gccaggtccg ccaggcgctt gccgaggccg atgccgccaa
     6541 gcgcgccacc ccggcgctgc accggctgtt ccaggatgcc ctcgcaaccg ccaagacggt
     6601 ggcctcgacg accgagctcg gcggtcgcgg ccgctcgatc gcctccgtcg gcctcgacct
     6661 gctcgaggcc cagcactccc ccgtcgctgg gtcgcaggcg ctcgttctgg gcacgggcag
     6721 ctacgccggc gtcgtcgtcg ccgagctcac ccggcgcggc tgcaccgaca tcgcggtcta
     6781 ctcccggacc ggccgcgccg actccttcgc ccgcactcac cccgtcacgc cggtccccga
     6841 gcccggcctc ctcgacgccc tcgcccgggc cgacctcgtc gtcacctgca gtggcgccag
     6901 cagcccgacg ctcaccgatg ccctcgtcaa ggcccagcgc aggtatgccg cgcgggtcct
     6961 gccgatcctc gacctgtcca cgggcggcga gctcggggac ggcgtggccg agctcgatga
     7021 ggccgacgtg ttcaccctcg acgagatcgg ccgcagcgcc ccggccgagc acgtcgacac
     7081 gcttctggcc gcgcaggacg tcgtcaaacg gtcggtcgcg gcatacctgc acgtcgaagg
     7141 cggtcgcacg gcggcaccgg ccatcaccgc catgcgtgct tacgtgagcg cgatcatcgc
     7201 ccgcgaggcc gaggtcgcgg cccgccgcta tccccccgag gtcgccgagg ccatcgatcg
     7261 ctccctgcgc cgggtgagcg gcgcgctgct gcacgccccg tcggttcgcg ccgcggagct
     7321 cgcccgcaca ggcgacctcg aggactacac gcacgcgatg gcgacgttgt tcggcatcgt
     7381 cgtcgaggcc gactcatggt gaggctgggg acgcgcggca gcctcctggc ccgcactcag
     7441 tccacccagg tcgcggaagc cctgtatgcc gcgaccggcc tgcgcgccga actcgtcatc
     7501 atccgctcag agggggacga ccaccggatc ccgctgcacg ctcccagccg gccggggatc
     7561 ttcgtcgcca cgttgcgcga cgccctgctg gcgggcaagg tcgacctcgt cgttcactcg
     7621 ttcaaggacc tgcccagcgc cccgatgccc ggcctggtca ttcccgcgat ccccgcgcgc
     7681 cgatccccgg ccgacgtgct cgtgacggcg accggaggtg gtctcgccga cctcgccgtg
     7741 ggtgccaggg tcgggacgag cagcccgcga cgcgctgccg cgctgctgcg ggtccgcccc
     7801 gacctcacga tcgtgccgat ccgcggcaac gtcgacacca gggtcgcggc ggtggctgcc
     7861 gggaccgtgg acgccgtcgt cctggcccag gccgggctcg aacggctcgg ccacctcacc
     7921 gaccggatga ccccgctgcc cacccaggtg atgcttcccg cccccgccca aggcgccctc
     7981 gccgtggaat gccgggcggc cgatcccctt gccgaccacc tcgcagtgct cgacgacccg
     8041 cgcgcccggc ttgaagtgac cgccgaacgc gccgtcctca ccggcgtcga ggccacctgc
     8101 acgaccgcca tcggcgccct ggcgcgttgg gagagtgggg cgctcacgct gagggccgaa
     8161 ctcaccgagg acggcacccg cagccatacc catgcggaac acagccggac cctcgccaat
     8221 gagcatgatc tcgacggcgc cgccgccctg ggtgcccagg tggcggagtg gctgctcttg
     8281 tgacggccac cccgaccggc gcggacctcc tccctggccc cgtcgttctc gtgcggccgg
     8341 accgcaacga ggcggatgcc gctgcgctgc gtgacgccgg gttcactgtg ctcgtcgagc
     8401 cctatctgag cgtgaccacg gccgacgacc cggcaccggc cctcgccctc ctcgacgtgc
     8461 tcgacccgcg ccgcaccggc cgcgacacct ggctcgtgct cacctctgca cgcaccatgg
     8521 cgcactgggc agcgctggtc ggccctcatc tcctgtatgc cgcgctcgac gccgcccacg
     8581 agggcggcct gcgggtggcc tgcgtcggat ctgctactgc agcaaccctt cccgacggga
     8641 tgagccccaa cctcacggcc accaactcgg acgcctcggc tctgctcgac gacctcctcg
     8701 ccgcaacggc atacccgatg ctggcgctgc tgcccggaag tgccatcgct cgcccgaccc
     8761 ttgcggacgg cctcaccgca gcgggctggg cggtccgcac ggcggcggtc tatgacacgc
     8821 gatcggtgct gacccggccc gcgagcgccg acctgctcgc cgccggcacc gccgccgcgc
     8881 tcgtcctgcg ttcgccgagt gcggtcgccg ccgtcgcggg tttcgtccag cccaacccct
     8941 ccgtcaccat cgtcgccgtc ggcccgacca cgaccgcggc cgtgcgtgag caggggtggt
     9001 ccgcgatcac ccctgccagc ccagattcgg gcgccgttgc tcgcgccctc tcccgcgccc
     9061 tcaccaggag ccagtgatgc cgacgtctga gcagtcccgc gtcaccgacc aacggc
//
LOCUS       DAOMZY010000123         9110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_7212_length_9110_cov_5.208283, whole genome shotgun sequence.
ACCESSION   DAOMZY010000123 DAOMZY010000000
VERSION     DAOMZY010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9110
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7212_length_9110_cov_5.208283"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PLK64_08355"
     CDS             <1..394
                     /locus_tag="PLK64_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79797.1"
                     /translation="QADKFFRDLGVLAYLDATGRPSDAGERDRIMTALQGFEPQGALP
                     SFALQTAAPPPPPPPGAAAQAPQGYAAQPPPPSPGGAAQPPPPPGWGAAPPPPPPPPS
                     WGAAPPPPPPPNWGAAPPPPPPSPPPAG"
     gene            complement(414..1757)
                     /locus_tag="PLK64_08360"
     CDS             complement(414..1757)
                     /locus_tag="PLK64_08360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HPV79798.1"
                     /translation="MQARQVRPLDAAYLALDGPRTVGHVCLVLPLDGPITLTQLREQV
                     GERVGRLPELRQRLHQGPNALDRPWWVDDPTFDIEAHVEEFHVGRRELSELVSELAME
                     ALPRTQPLWQVHLLREGRRCYVMTKVHHSIADGSRYRDILHALFGTAQDDVPPWSPQR
                     IPSAFDLATRGLVGASRWAWTSVYAAARAIADNPQSLGGVLNSVGVHGAPPTPFNRSV
                     GPNRVWAFRTWGLAASKPTRAHFGVTVNDVMHAVVAAGLRQWLIEQNALPTSPLVALV
                     PMSVRHLTTDPSGANRLSLGLCRIPTDIEDPVERIFSAREDMAEAKERPVLGEGALEV
                     LTRLLGPTLEPASWVASTVRLLDTVRLPFNTIISNVPMGQDLFVIAGRRVTAVYPFAP
                     LGDGMGVSITIQGYQGRLDVGISACADLLPDVEHLMDLMTAEYAHVCTLASLTGG"
     gene            complement(1859..2878)
                     /locus_tag="PLK64_08365"
     CDS             complement(1859..2878)
                     /locus_tag="PLK64_08365"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HPV79799.1"
                     /translation="MSSDTPASEYRIRATAEDVRRMMSRLLLTEGSDTRRKMSAFWVL
                     LVLATVIATSGVVADSTATVIGAMIVAPLMTPILGTALSVVLANRRLVAVNFGLVIVG
                     ALCVIAIAYLIGMTSQMHLVADSNSQIAGRVSPRLIDLVGALATGAVGAFATVRSDVS
                     DTLPGVAIAISLVPPLAVVGLTLESGEPSQALGALLLFGTNVSAIIATGTVVFLSYRV
                     RQAAIDAGLPIGRLHRGALVTAFASVVILAIPLSLGSSVVLQQEVLVSKATPVARAWA
                     KAHGGQISDVTYRDNSLRILAIGLLTLDQVTGLRPDLDAAGLGDVDVLVTLVAGGTKE
                     LPGKP"
     gene            3067..3645
                     /gene="pyrR"
                     /locus_tag="PLK64_08370"
     CDS             3067..3645
                     /gene="pyrR"
                     /locus_tag="PLK64_08370"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HPV79800.1"
                     /translation="MVASEQTPSPTPPGARVVMSSGDVSRALRRVAHEILERNKGPAD
                     LVLLGIPSRGVPLAHRLASVIAEVEGVAVPVGSLDVTMHRDDLRRQPTRTVTPTKIPP
                     SGVDGKIVVLVDDVLYSGRTIRAALEALTELGRPRAVRLAVLVDRGHRELPIRADHVG
                     KNLPTSSSERVHVVLAENDGGDDEVRISGGDR"
     gene            3642..4610
                     /locus_tag="PLK64_08375"
     CDS             3642..4610
                     /locus_tag="PLK64_08375"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HPV79801.1"
                     /translation="MTRKHLLSSADLSRDEVDSILDTAASMHDVQRREVKKLPTLRGR
                     TVINLFFEDSTRTRSSFEIAGKWMSADTINITGKGSSTSKGESLRDTVMTICAMSVDA
                     LVIRHSASGACHQVAQWVDASVINAGDGTHEHPTQALLDAYTLRQRLGSLEGRHIVIV
                     GDLAHSRVFRSNVITLGRLGARVTVVAPPTLMPSGIGDWAARDGFATSWDLDQTLYAA
                     SPADRPDAVMMLRVQRERMSGGFFPTEREYTTGYGLTRERLARLYAATPDLPILHPGP
                     MNRGLEISAEAADATSSAILDQVSAGVAVRMAVLYHLLAGETEAAA"
     gene            4607..5938
                     /locus_tag="PLK64_08380"
     CDS             4607..5938
                     /locus_tag="PLK64_08380"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPV79802.1"
                     /translation="MTDLLLKGANLLGDKGADVLVREGVIAEIGSIPAAGELDGRRLT
                     VVDAAGLVLLPGLVDLHAHLREPGREDAETVASGSAAAAAGGFTAILAMANTSPVTDT
                     AEAAERVFDLGRAAGLVDVQPIGAVSKGLAGAELAELGLMARSRARVRVFSDDGKCVH
                     DARLMRRALDYVKAFGGTISQHAQDPRLADGSACCHEGEISGRLGLPAWPAIAEDVVV
                     ARDVQLARHTGSRVHVAHVSTAASVEIIRWAKAQGIAVTAEVTPHHLALGTDLLTGYD
                     PVFKVNPPLRPDEDRAAILAALVDGVVDAVATDHAPHARHDKEHAFPDAAFGMLGLET
                     ALAVVAEVAVRTGLLSWADVADRMSSAPARIAGLADQGRPIAVGEPANLVLVDPDASW
                     TVDRSTSQSLSRNNPWHGRTLPAPVVATFLRGECTAYRGRVRGQFDPEEIA"
     gene            5935..7116
                     /gene="carA"
                     /locus_tag="PLK64_08385"
     CDS             5935..7116
                     /gene="carA"
                     /locus_tag="PLK64_08385"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HPV79803.1"
                     /translation="MSPATSLRRHREPAALVLEDGRTFLGESFGAVGETLGEAVFSTG
                     MTGYQETLTDPSYHRQVVVMTAPHVGNTGWNDEDDESSRIWVAGYAVRDPALRPSNWR
                     SARSLEDELVEQGVVGICGIDTRALTRHLRERGAMRVGIFSGAAAAPSQADLLAKVLA
                     APGMAGAELASEVSTERPYVVAAHGERRFTVAAIDLGIKGMTPALMAQEGIEVHVLPA
                     TATIADVLAVGGADGSPGPDGVFFSNGPGDPATADLTLLREVLDRRIPFFGICFGNQV
                     LGRALGFGTYKLKYGHRGINQPVMDRTTGKVEVTAHNHGFAVDAPIKHDSQTPYGRVR
                     VSHICLNDEVVEGLECLDLAAFSVQYHPEAAAGPHDAAYLFDRFCALMRTHPWKGAQH
                     A"
     gene            7109..>9110
                     /gene="carB"
                     /locus_tag="PLK64_08390"
     CDS             7109..>9110
                     /gene="carB"
                     /locus_tag="PLK64_08390"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPV79804.1"
                     /translation="MPRREDITSVLVIGSGPIVIGQACEFDYSGTQACRVLRDEGIRV
                     VLVNSNPATIMTDPEFADATYVEPITPEIVEAIIAKERPDAVLATLGGQTALNCAMAL
                     HERGVLERYNCPLIGANVEAIQLGEDREKFKGVVARCGAESARSIICHTMDECLAAAE
                     ELRYPVVVRPSFTMGGLGSGFAYDEADLRRIGGAGLQYSPVTEVLLEESIIGWKEYEL
                     EVMRDHKDNVVVVCSIENLDPMGVHTGDSITVAPALTLTDREYQRLRDIGIAVIREVG
                     VDTGGCNIQFAVNPDDGRIIVIEMNPRVSRSSALASKATGFPIAKIAAKMAIGYTLDE
                     VPNDITKQTPASFEPTLDYVVVKVPRFAFEKFPAADPTLTTTMKSVGEAMSLGRNFTE
                     ALQKALRSMERKEASFHWDGPVPDAAAVEALLAQIAVPTDGRIIGVQQALRGGATVEQ
                     VYAATGIDPWFLDQIALINEVAESVRTAERLKPELLALAKRHGFSDRQLASLRAMPEA
                     VVRGVRHALGIRPVYKTVDTCAAEFAAATPYYYSSYDSETEVLPRERPAVIILGSGPN
                     RIGQGVEFDYSCVHASFALRDAGYDTVMVNCNPETVSTDYDTSSRLYFEPLTLEDVLE
                     VVHAEAAAGPIAGVVVQLGGQTPLGLAQALKDEGVLVVGTSPE"
BASE COUNT         1390 a         3258 c         3127 g         1335 t
ORIGIN      
        1 acaggcggac aagttcttcc gcgacctcgg ggtgctggcc tacctcgacg ccacgggtcg
       61 gccgtccgac gcgggggagc gagaccggat catgaccgcg ttgcagggct ttgagcccca
      121 gggcgccctg ccctcgttcg cgctgcagac cgccgcgccg ccgccgcccc ctccgcccgg
      181 cgcggctgcc caggctccgc aggggtatgc cgcccagcct ccgcctcctt ctcccggtgg
      241 cgctgcccag cctccgccac caccaggttg gggtgccgcc ccgccgcctc cgccaccacc
      301 gcccagctgg ggcgccgcgc cgcctccgcc gcccccgccg aactggggtg ctgctccgcc
      361 acctccgcct ccgtcaccac cgccggccgg gtagggacgg cagcggccgg agctcagccg
      421 ccggtgaggg acgccagcgt gcagacgtgg gcgtattcgg ccgtcatcag gtccatgagg
      481 tgctcgacat cgggcaggag atcggcgcat gcgctgatcc cgacgtccag ccgaccctga
      541 tagccctgga tggtgatgct cacccccatg ccgtcgccca acggcgcgaa cgggtagacg
      601 gccgtcaccc ggcgaccggc gatgacgaac aggtcctgcc ccatggggac gttcgagatg
      661 atcgtgttga acggcagccg gacggtgtcg agcagccgca cggtcgacgc gacccaactc
      721 gccggctcca aggtcgggcc caacaaccgg gtgaggactt caagggctcc ttcgccgagg
      781 actggtcgct ccttggcttc ggccatgtcc tcgcgggccg agaagatccg ctcgaccggg
      841 tcctcgatgt cggtcgggat ccggcacagg ccaagcgaga gccggttggc ccccgagggg
      901 tcggtcgtca gatgtcgcac cgacatcggc acgagcgcca cgagagggct ggtcggcagc
      961 gcgttctgct cgatgagcca ctgccgcagg ccggcagcca caacggcatg catgacgtcg
     1021 ttgaccgtga ccccgaagtg cgcccgcgtg ggcttgctcg ccgccagccc ccacgtgcgg
     1081 aacgcccaga cccggttggg tcccacggaa cggttgaacg gggtcggcgg cgcgccgtgg
     1141 acgccgaccg agttgaggac gccgcccagc gactgggggt tgtcggcaat ggcgcgggcg
     1201 gcggcataca ccgaggtcca ggcccagcgc gaggccccga cgagcccgcg ggtggccagg
     1261 tcgaaggcgc tggggatgcg ctggggtgac cacgggggga cgtcgtcctg cgcggtcccg
     1321 aacagcgcgt ggaggatgtc gcggtagcga gacccgtcgg cgatgctgtg gtggaccttc
     1381 gtcatgacgt agcagcggcg gccttcgcgc aggaggtgca cctgccaaag cggctgggtg
     1441 cgcggcagcg cctccatcgc caactcggac accaactcgc tgagctcgcg ccgcccgacg
     1501 tggaactcct cgacgtgggc ctcgatgtcg aaggtcgggt cgtcgaccca ccagggccgg
     1561 tcgagggcgt tgggcccctg gtgcaggcgc tggcgcaact ccggcagccg cccgacccgc
     1621 tcgccgacct gctcgcgcaa ctgggtcagc gtgatcggcc cgtcgagggg caggacgagg
     1681 cagacgtggc cgaccgtgcg gggtccgtcg agcgcgaggt atgccgcgtc caggggacgc
     1741 acctggcgag cctgcatccg ttgccgcctc cttccgagtg cgaccctagt tggttcgtcc
     1801 ccggcctgtc cctggtgagc gcgcaacgcc atatcgcaac acgacctgac ctaccccgct
     1861 acggcttgcc gggcagttcc ttcgttccgc cggcgacgag ggtcaccagc acgtcgacat
     1921 cgcccagccc ggcggcgtcg aggtccggac gcaggccggt gacctgatcg agcgtgagca
     1981 ggccgatggc gaggatccgc agggagttgt cgcggtaggt gacatcactg atctggccgc
     2041 cgtgggcctt ggcccaggcc cgggcgaccg gggtcgcctt ggagacgagc acctcctgct
     2101 ggagcacaac cgagctgccg agggacaggg ggatggcgag gatgacgacc gacgcgaagg
     2161 cggtgacgag cgcgcctcgg tgcagccgcc cgatgggcag acccgcatcg atcgccgcct
     2221 gacgcacgcg gtagctgagg aacaccacgg tgccggtggc gatgatcgcg ctgacgttgg
     2281 tgccgaagag cagcagcgca ccaagcgcct gggacggctc gcccgactcg agggtgaggc
     2341 ccacaacggc caacgggggc acgagtgaga tggcgatcgc cacgccgggc aaggtgtcgg
     2401 acacgtcgga gcgcaccgtc gcgaacgccc ccacggcgcc cgtcgccagg gccccgacga
     2461 gatcgatgag ccgcgggctg acccgcccgg cgatctgcga gttgctgtca gcgacgaggt
     2521 gcatctgcga cgtcatcccg atgagatagg cgatggcgat gacgcacaag gcccccacga
     2581 tcaccagccc gaagttgacc gcaacaagac gccggttggc gagcacgacc gacaaggcgg
     2641 tgccgaggat gggcgtcatc aggggagcga cgatcatcgc gccgatcacg gtcgccgtgg
     2701 aatcggccac gacacccgac gtggcgatga cggtggccaa gaccaagagc acccagaacg
     2761 ctgacatctt ccttctcgtg tcgctgccct cggtgaggag caggcgggac atcatccggc
     2821 gcacgtcctc ggccgtggcc cggatgcggt attcgctggc gggagtgtcg gaactcatgc
     2881 gtcaagtgtg acgcgcgacc ccccgcttcg cacgagttgt gagggcacct cccggggccc
     2941 ggctggaagc ggcggaggcg accggcatac ggaccctgcg cgaggcgacc cgacgccgag
     3001 agtagggtta cccgcgaacc agacatcctt taaccaccgt cctgtgaggc ggggaaggag
     3061 gtccgcgtgg tggcgtccga acagaccccc tcgccgacac ccccaggcgc acgtgtcgtc
     3121 atgagctccg gtgacgtttc ccgggccttg cgccgagtgg cccacgagat cctcgaacgc
     3181 aacaagggtc cggccgacct cgtgctgctg ggcatcccgt cccgtggcgt gccgctggcg
     3241 caccgcctcg cgagcgtcat cgccgaggtc gagggagtcg cggtccccgt cggctcgctc
     3301 gatgtgacca tgcaccgcga cgatctgcgc cgccagccca cccgcaccgt aaccccgacg
     3361 aagatcccgc cgtcaggggt cgacgggaag atcgtcgttc tcgtcgacga cgtgctctac
     3421 tccgggcgga cgatccgggc cgccctcgag gcgctcaccg agctcggccg cccccgggcg
     3481 gtgcgcctgg ccgtcctcgt cgaccgtggg caccgcgagc tgcccatccg cgccgaccac
     3541 gtcggcaaga acctccccac cagcagttcc gaacgcgtcc acgtcgtgct ggccgagaac
     3601 gacggcggcg acgacgaagt gcgcatcagc ggaggcgacc gatgacccgc aagcacctgt
     3661 tgtcctccgc cgacctgtcc cgcgacgaag tcgactcgat cctcgacacc gccgcctcga
     3721 tgcacgacgt gcaacgccgg gaggtcaaga agctcccgac cctgcgtggg cgcacggtca
     3781 tcaacctgtt cttcgaggac tccacccgca cccggtcgtc gttcgagatc gccggcaagt
     3841 ggatgtcggc cgacaccatc aacatcaccg gcaagggctc ctcgacgtcc aagggcgagt
     3901 cgctgcgcga cacggtcatg acgatctgcg ccatgtcggt cgacgctctc gtcatccggc
     3961 acagtgcctc aggggcctgt caccaggtcg cccagtgggt cgacgcgtcg gtcatcaacg
     4021 ccggtgacgg cacccacgag caccccaccc aggcgctcct ggatgcctac acgttgcgtc
     4081 agcggctcgg gtcgctcgag ggtcggcaca tcgtcatcgt cggcgacttg gcccactcgc
     4141 gggtgttccg ctccaacgtc atcacccttg gccggctggg tgcgcgcgtc accgtcgtcg
     4201 ccccgccgac gctgatgccc agcgggatcg gcgactgggc ggcccgcgac ggtttcgcca
     4261 cgagctggga cctcgaccag actctgtatg ccgcgtcgcc cgccgaccgc cccgacgcgg
     4321 tgatgatgct gcgggtccag cgcgagcgga tgagcggcgg gttcttcccg accgaacgcg
     4381 aatacaccac gggctacggc ctcacgaggg agcgcttggc ccgactgtat gccgccaccc
     4441 ccgacttgcc gatcctgcac cccgggccga tgaaccgagg cctggagatc tcggccgaag
     4501 ccgccgatgc gacgagctcg gcgatcctcg accaggtgtc tgccggagtg gccgtgcgga
     4561 tggcggtcct ctatcacctc ctggccggcg agacggaggc tgccgcatga ccgacctcct
     4621 gctcaagggc gcaaacctgt tgggagacaa gggcgctgac gtcctcgtcc gcgagggtgt
     4681 catcgccgaa atcgggtcta tccccgctgc gggcgaactc gacggacggc ggctcaccgt
     4741 tgtcgacgct gccgggctcg tcctgctgcc gggcctggtc gacctccatg cccaccttcg
     4801 tgagcccggt cgcgaggacg ccgagaccgt cgcgagcggc tcggccgctg cggccgcggg
     4861 tggtttcacc gcgatccttg cgatggccaa cacctctccg gtgaccgaca cggccgaggc
     4921 ggccgagcgg gtgttcgacc tcggtcgggc ggccgggctg gtcgacgtgc agccgatcgg
     4981 cgccgtctcc aaggggctgg ctggcgccga gctcgccgag ctcgggttga tggcccgctc
     5041 tcgggcccgg gtccgggtgt tctccgacga cggcaagtgc gtccacgacg cccgcctcat
     5101 gcgtcgggcc ctcgactacg tcaaggcgtt cgggggcacc atctctcagc acgcccagga
     5161 cccccggctc gccgacgggt cggcctgctg ccacgagggc gagatctccg gtcggctcgg
     5221 gctgccggcc tggcccgcga tcgcggagga cgtcgtcgtc gcgcgcgatg tccagctggc
     5281 gcggcatacc ggctcgcggg tccacgtcgc gcacgtgtcg acggcggcgt cggtcgagat
     5341 tatccgctgg gccaaggccc aggggatcgc cgtcaccgcc gaggtgactc cccaccatct
     5401 ggcgctgggc actgacctgc tgaccggcta tgacccggtc ttcaaggtca acccgccgct
     5461 gcgacccgac gaggaccgag cggccatcct ggcggcgctc gtcgacggtg tcgtcgatgc
     5521 cgtcgccacc gaccatgccc cgcacgcccg gcacgacaag gagcacgcgt tcccggatgc
     5581 ggcgttcggg atgctcggtc tggagacggc gctcgccgtc gtcgccgagg tggccgtccg
     5641 gacggggctg ctgagctggg ccgacgtggc cgaccggatg tcctccgccc ccgcgaggat
     5701 cgccgggctg gccgatcagg gccggccgat cgccgtgggc gagccggcca acctcgtgct
     5761 cgtcgacccc gacgcgtcgt ggaccgtcga ccggtccacc agccagtcgc tgtcccgcaa
     5821 caacccctgg cacgggcgca ccctgcccgc cccggtcgtc gcgacgttcc tacgcggcga
     5881 gtgcacggca taccgaggtc gcgttcgcgg ccagttcgac ccggaggaga tcgcgtgagt
     5941 cccgcaacgt cgctgcgcag gcatcgggag cccgcagcgc tggttcttga ggacggtcgc
     6001 acgttcctcg gtgagtcctt cggcgccgtg ggggagaccc tcggggaggc ggtcttctcc
     6061 acggggatga ccggctacca ggaaaccctc actgatccgt cctaccaccg gcaggtcgtc
     6121 gtcatgacgg caccgcacgt cggcaacacc ggctggaacg acgaggacga cgagtcctcg
     6181 cgcatctggg tcgccgggta tgccgtgcgc gaccctgcgc tgcgcccgtc caactggcgc
     6241 tcggcccggt ccctcgagga tgagctcgtc gagcaggggg tcgttgggat ctgcggcatc
     6301 gacacccgcg ccctcacccg tcacctgcgc gaacgcgggg cgatgcgggt cggcatcttc
     6361 agcggcgcgg cggcggcgcc gagccaggcc gacctgctgg ccaaggtctt ggccgcgccg
     6421 gggatggccg gtgccgagtt ggcctcggag gtgtcgaccg agcggccgta tgtcgttgcc
     6481 gcccatgggg aacggcgttt cacggtggct gcgatcgact tggggatcaa gggcatgacc
     6541 cccgccctga tggcccagga gggcatcgag gtccacgtgc tcccggcgac ggcgaccatc
     6601 gccgacgtgc tggcggtcgg gggagcggac gggtcacctg gccccgacgg ggtcttcttc
     6661 tccaacggcc cgggcgaccc ggcgaccgcc gatctcaccc tgctgcgcga ggtcctcgat
     6721 cggcggatcc cgttcttcgg catctgcttc ggtaaccagg tgcttggccg cgccctcggc
     6781 ttcggcacgt acaagctgaa gtacggccac cgggggatca accagccggt gatggaccgc
     6841 accaccggca aggtcgaggt gaccgcccac aaccacgggt tcgccgtcga cgccccgatc
     6901 aagcacgact cgcagacccc ttatgggcgg gtgcgggtca gccacatctg cctcaatgac
     6961 gaggttgtcg agggcctgga gtgcctcgac ctggctgcct tctccgtgca gtaccacccg
     7021 gaggccgccg cggggccgca cgacgcggca tacctcttcg accgcttctg tgcgctcatg
     7081 cgcacccacc cctggaaggg ggcccagcat gcctaggcgc gaagacatca cgagcgtcct
     7141 ggtcatcggg tccggcccga tcgtcatcgg gcaggcctgc gagttcgact actccggcac
     7201 ccaggcgtgt cgcgtgctgc gcgacgaagg gatccgcgtc gtcctggtca actccaaccc
     7261 ggcgacgatc atgaccgacc ccgagttcgc cgacgcgacc tacgtcgagc cgatcacccc
     7321 ggagatcgtc gaggcgatca tcgccaagga acgacccgac gccgtgctgg cgaccctcgg
     7381 cggtcagacg gcgctcaact gcgcgatggc cctgcacgag cgcggggtgc tggagcgcta
     7441 caactgcccg ctcatcggag ccaacgtcga ggcgatccaa ctcggcgagg accgcgagaa
     7501 gttcaagggg gtcgtcgccc gttgcggggc cgagtcggct cgctcgatca tctgccacac
     7561 gatggacgaa tgcctcgctg cggcagagga attgcgctac cccgtcgtcg tgcggccgtc
     7621 cttcaccatg ggcggtctgg ggtcgggctt cgcctacgac gaggccgacc tgcgccggat
     7681 tggcggcgcc gggctgcagt actccccggt caccgaggtg ctccttgagg agtcgatcat
     7741 cggctggaag gagtacgaac ttgaggtgat gcgcgaccac aaggacaacg tcgtggtcgt
     7801 ctgctccatc gagaacctcg accccatggg cgtccacacg ggtgactcga tcaccgtggc
     7861 tccggcgctc accctcaccg accgcgaata ccagcggctg cgcgacatcg gcatcgctgt
     7921 cattcgcgag gtcggcgtcg acaccggggg ctgcaacatc cagttcgcag tcaaccctga
     7981 cgacggccgg atcatcgtca tcgagatgaa cccgcgggtc tcgcgctcgt cggcgctggc
     8041 ctcgaaggcg accggcttcc cgatcgccaa gatcgccgcg aagatggcga tcggctacac
     8101 ccttgacgag gtccccaacg acatcaccaa gcagacgccg gcgtccttcg agccgaccct
     8161 ggactatgtc gtcgtcaagg tgccgcggtt cgcgttcgag aagttccccg ccgccgaccc
     8221 gaccctgacg acgacgatga agagcgtcgg cgaggcgatg tcgctgggcc gcaacttcac
     8281 cgaggcgctg cagaaggcgc tgcgctcgat ggagcgcaag gaggccagct ttcactggga
     8341 cggcccggtt cccgacgcgg cggcggtcga ggcgttgctc gcccagatcg cggtccccac
     8401 cgacggtcgg atcatcgggg tccagcaggc tctgcgtggc ggggcgaccg tggagcaggt
     8461 gtatgccgcg accggcatcg acccgtggtt cctcgaccag atcgccctta tcaacgaggt
     8521 cgccgagtcc gtgcgcaccg ccgagcggct caagcccgag ttgctggcgc tcgccaagcg
     8581 acacgggttc tccgaccggc agttggcctc actgcgggcc atgccggagg ccgtcgtccg
     8641 gggtgtgcgc cacgcgctcg gcatccggcc ggtctacaag accgtcgaca cctgcgccgc
     8701 cgagttcgcg gccgcgacgc cctactacta ctcgtcctac gacagcgaga ccgaggtgct
     8761 gccgcgggag cggccggccg tcatcatcct cggctccggc cccaaccgga tcggtcaagg
     8821 cgtcgagttc gactactcct gtgtccatgc ctcgttcgcg ttgcgcgacg cgggctacga
     8881 cacggtgatg gtcaactgca accccgagac cgtgtcgacc gactacgaca cctcgagccg
     8941 gctctacttc gaaccgctca cccttgagga cgtcctcgag gtcgtccacg cagaggctgc
     9001 ggcaggccca atcgcggggg tcgtcgtcca actcggcggg cagaccccac tcgggctcgc
     9061 ccaggctctc aaggacgagg gagtcctcgt cgtcggcacc tcgcccgagg
//
LOCUS       DAOMZY010000124         9077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_7250_length_9077_cov_6.170472, whole genome shotgun sequence.
ACCESSION   DAOMZY010000124 DAOMZY010000000
VERSION     DAOMZY010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9077
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7250_length_9077_cov_6.170472"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1409
                     /locus_tag="PLK64_08395"
     CDS             <1..1409
                     /locus_tag="PLK64_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HPV79805.1"
                     /translation="AVSGRHTRGMSFLGDQPLVVTREDGVATLWLNRPEKRNAVDYAM
                     WLELARLAAELGADPDVRVLVVRGVGKHFCAGGDISGLGDLPFEEYTRANELADRALA
                     DFPKPSIAFIQGSCIGGGAEIAIACDLRLADASARFGITPARLGIIYPGFAVARAVQL
                     IGESATKHLLFTAQIVDADRALRVGLIDELLATEVAEARLAELVRVMSRERSLLTQMA
                     SKEMVDSVLAHGHVTDEVQARWAAALATSPDAAEGIAAFTERRAPDFRWAPPVDWAAH
                     SDRRADPPAEPTPIEITAPVLGVDDCAAGWVGALLAPGLPRPQLYAADTIASLVETVM
                     ESWPVAVVAIDRSAGLTDGDTRAAKDLEVEAWVRTRPPAHMLNVHAETSFARMAGEPI
                     AASKKTPEGIDARMAQLARHGIARPSVLSDSGFAADDVLEACAAAWTAARFANGDARS
                     LPDAPETFSDGIPAAIWA"
     gene            complement(1507..>2177)
                     /locus_tag="PLK64_08400"
     CDS             complement(1507..>2177)
                     /locus_tag="PLK64_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV79806.1"
                     /translation="AEPPSAEPPAAETPATEPSSAEGAGQGRRARARELRRLLPAAVR
                     AALATASGAKALSGFVTLFLSFLLWDQPLPGISSVLALGLVVAAAGVGNALGSFAGNR
                     IGDRSPTVIASAGLLAAIAVGALVTAYYSVPTLILLGLVSGAFGQLAKLCLDALIQDA
                     TDDETRAQVFSWSETRLQAAWVGGGALGIAMPLIARLGFGAVTAMLVGVLVAGIVIRS
                     RSGT"
     assembly_gap    2178..2277
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2278..3296)
                     /locus_tag="PLK64_08405"
     CDS             complement(<2278..3296)
                     /locus_tag="PLK64_08405"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV79807.1"
                     /translation="MDTGEPAMRDAGADPGSRLGAAAGPGAGTGAAGEPQPRQPLRND
                     ATDQPALVEVASERARRAAAVTGRATARAGKAAGRATAGASKATAKGVVVGAKFMGRG
                     AKAGTERFRLFARSEGAAESGLSRVTEMQVLASAGDAAFTVALASTILALPLGQARGQ
                     VALFLVTTMAPFVVLAPLVGPLLDRWRHGRRWAIGTTLALRAFLSWVLAGVVADGSPW
                     LLPLALLCLMATRAYAVALSAGIPLVRPDAISLVTANSRQSVATLMGMLLGAAVAAPI
                     GRFGAPWSLRVAFLIYIGATVLAIRLPAAVDSQRTPPPDVATESPPAEPPSAEPPAAE
                     TPATEP"
     gene            3619..6102
                     /locus_tag="PLK64_08410"
     CDS             3619..6102
                     /locus_tag="PLK64_08410"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HPV79808.1"
                     /translation="MGPAFPPTHPLALSPTVAPERTVDGFVQDYLRELNFGQGAALTR
                     STVNHQYLALARTVRHHLMAAWLETAQRRREHPTKMVGYLSAEYLLGRQLGNALMATD
                     LRDVVNEAMKACGLDLRKVRVQEVEPGLGNGGLGRLAACFIDSLATMDVSCIGYGIRY
                     EYGIFRQTFVDGRQAEVPDNWLSLGAPWEFPNPERAVRVDFGGRVEDVVDEAGVTRRQ
                     WVPAWNVLGIPYNYMVPGYRNGVVNTLRLWSAEATRAFDLTIFNSGDYAQAVRAQTFA
                     ENISKVLYPEDSTPQGRELRLQQQYFFVACSLRDYLDGTLPQGFDLRKLPEQIIFQLN
                     DTHPVIAIPEFMRLLVDEKGMEWDEAWAITQKCFAYTCHTLLPEALEVWPTDLLGRLL
                     PRHLEIIYRINEEFLASVRAAYPGDEMRARRMSIISDYPQAVRMAHLATVTAVRVNGV
                     AELHSQLLRDKVLPDFSAFWPEKFTNVTNGITPRRFIRMANPALSQVITDAIGEGWVT
                     DLERLRELEPYVDDPAFRAEFRRAKAANKKRLYTLAEAFEGVSLPEGHLIDVMVKRLH
                     EYKRQLLKVLHVVSTYEGILSGAVRAEDITPRLVVFGAKAAPGYFMAKEIIYLINRMA
                     TVINNDPLVKRQLIVGFPANYNVTLAEKIIPAADLSEQISLAGKEASGTGNMKFTLNG
                     ALTIGTDDGANVEIRRLVGDDNFFLFGMREPEVVALQAKGYVPSSYYESNPTLRRAID
                     LIASGHFTEGNRDAVGPIIGDLLSNDRFLVLADYQSYIDAQARVDTAYRDEEGWTRSA
                     ILNVARAGFFSSDRSMRDYIDRVWGLRQS"
     gene            6146..6541
                     /locus_tag="PLK64_08415"
     CDS             6146..6541
                     /locus_tag="PLK64_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPV79809.1"
                     /translation="MSAVIHFEIHANDVPRAVAFYQAIFGWEVQDWSEYAGMPYFGVV
                     TRPEGEPGINGAIMARMGPGPQPGGPVGGAVLTVGCEDFDGTAEKVLQAGGTVALAKH
                     ALPGMAWQGYFLDTEGNVFGVHQPDVDAQ"
     gene            6556..6687
                     /locus_tag="PLK64_08420"
     CDS             6556..6687
                     /locus_tag="PLK64_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79810.1"
                     /translation="MSELVDLLGQRRDAAEVVEAVRQFGLDEVMDDPPSCQYVMSRR"
     gene            complement(6761..7186)
                     /locus_tag="PLK64_08425"
     CDS             complement(6761..7186)
                     /locus_tag="PLK64_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPV79811.1"
                     /translation="MKIVDTNVLLYAVSERSAHHASARGWLTQALHGPEVIGMPWVTL
                     LGFIRILTHPRVFPCPLTAAQATELVQTWLSAPAVVIPEPSARHLSLLAGLLRESGTA
                     GNLTTDAHLAALAIESSATVVTFDRDFGRFGIPVETPQI"
     gene            complement(7183..7440)
                     /locus_tag="PLK64_08430"
     CDS             complement(7183..7440)
                     /locus_tag="PLK64_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79812.1"
                     /translation="MSRTTVTLEPDVEALVHRAMRERGVSFKEALNSAIRAGLAPAAP
                     ALDLRFPTFDMGEPFVDLSHAAAFVAELEADEIGQDLARGR"
     gene            complement(7583..8452)
                     /locus_tag="PLK64_08435"
     CDS             complement(7583..8452)
                     /locus_tag="PLK64_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HPV79813.1"
                     /translation="MTIRTLHTVHRGDGFHWVGDGFYVTQLLPASPELQRLTDPFLLM
                     DYNPVKEYPPTTTPRGVGVHPHRGFETVTMAFEGAVAHHDSTGAGGVIRPGDVQWMTA
                     ASGILHKEYHEPEWAARGGRFHMMQLWVNLPAAHKMDAPGYQPLTAESMGKVKLDDGG
                     VVTLVAGELDDVRGPARTVTPISLWDVQLGADESADLAVPDGHTLMTFVLDGELTTST
                     GTIGREEMGIFNREGDRLQVTAGEVGARFLVLGGKPIDEPVVFYGPFVMNTMEEIREA
                     MVDFNNGKFGTLV"
     gene            complement(8515..8856)
                     /locus_tag="PLK64_08440"
     CDS             complement(8515..8856)
                     /locus_tag="PLK64_08440"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPV79814.1"
                     /translation="MDRTDLYNRYRTAELLFSQRRYAQAALLLEELLQDLQDLPEQTG
                     TGEARLLLARAYYHSAQLGRAERAARELVAADPSDPYAVLLLGRVLCRQARGDEGKGW
                     LRRAEALGFTV"
BASE COUNT         1485 a         3102 c         3052 g         1338 t
ORIGIN      
        1 gggcggtgag cgggcggcat actcggggca tgtctttcct cggtgaccaa ccgctcgtcg
       61 tcacccgtga ggacggcgtc gccaccctct ggctcaaccg gcccgagaag cgcaacgccg
      121 tcgactacgc gatgtggctg gaactcgcgc ggctggcggc cgagctcggc gccgaccctg
      181 acgtgcgcgt cctcgtggtg cgcggcgtgg gcaagcactt ctgcgccggc ggcgacatca
      241 gcggtctggg cgacctgccg ttcgaggagt acacccgcgc caacgagctc gccgatcggg
      301 cgctggccga cttccccaag ccgagcatcg ccttcatcca agggtcgtgc atcggcggag
      361 gcgccgagat cgccatcgcc tgcgatctgc ggctggccga cgcgagcgca cggttcggga
      421 tcacgcctgc gcgcctgggc atcatctacc ccggcttcgc ggtggcgcgc gcagtgcagc
      481 tcatcggcga atcggccacc aaacacttgc tgttcaccgc tcagatcgtc gatgccgacc
      541 gagcgctgcg ggtcgggctc atcgacgagt tgctcgcgac ggaggtggcc gaggcgcggc
      601 tggccgagct ggtccgggtc atgtcccgcg agcgctcgct gctcacccag atggccagca
      661 aggagatggt cgacagcgtg ctcgcccacg gccacgtcac cgacgaggtt caggcgcgct
      721 gggccgccgc cttggcgacc agccccgacg ccgccgaggg gatcgccgcc ttcaccgagc
      781 ggcgcgcgcc tgacttccgg tgggcaccgc cggtggactg ggcagcccac agcgaccgcc
      841 gcgcagaccc gcctgctgag cccaccccga tcgagatcac cgccccggtg ctcggagtgg
      901 acgactgcgc cgccggctgg gtcggcgccc tcctcgcacc cgggctcccc cgaccccagc
      961 tgtatgccgc ggacaccatc gcctccctcg tcgagaccgt catggagtcc tggcccgtag
     1021 cggtggtcgc catcgaccgg tcggccggtc tgacggacgg cgacacccgc gcggccaagg
     1081 atctcgaggt cgaggcgtgg gtgcgcaccc ggccacctgc ccacatgctg aacgtgcacg
     1141 ctgagacgtc tttcgcgcgg atggccggcg agccgatcgc ggcgtccaag aagacgcctg
     1201 agggcatcga cgcccggatg gctcagttgg cacgacacgg catcgcccgt cccagtgtcc
     1261 tctccgacag cggttttgcc gccgacgacg ttctcgaggc ctgtgccgcc gcgtggacgg
     1321 ccgcccggtt tgccaacggg gacgcgcggt cgttgcctga cgcacccgag accttctcgg
     1381 acggcatacc ggccgcgatc tgggcctgag gagtcttcgc gctcgtcagg tgagcaaaac
     1441 cgacggcagg acgtcggaat cgctcacccg aaatccgtca gccacccagc gccctggtgc
     1501 gcgtcgtcag gtgccggatc ggctgcggat gacgatcccg gccacgagga cgccgaccaa
     1561 catcgcggtg acggcgccga acccgagtcg ggcgatcagc ggcatggcga tccccagcgc
     1621 accgccgccg acccaggccg cctgcagtcg cgtctccgac cacgagaaca cctgcgctcg
     1681 cgtctcgtcg tcggtcgcgt cctggatgag ggcgtccaga cagagcttgg ccaactgccc
     1741 gaatgccccg ctgaccaggc caagcaggat cagcgtgggc accgagtagt aggccgtcac
     1801 gagggcaccc acggcaatcg ccgcgagcag ccccgcggag gcgatcacgg tcggggagcg
     1861 gtcgccgatc cgattgccgg cgaaggagcc cagggcgttg ccgactcccg ctgccgcgac
     1921 caccaggccc agggcgagca cgctcgagat gccggggagc ggctggtccc agagcaggaa
     1981 cgacaggaac aacgtgacga accccgacag cgccttggcc ccagacgccg tcgcgagggc
     2041 ggcgcggact gccgcaggca acaaccgccg caactcccgg gcccgggccc gccgcccttg
     2101 cccggcgccc tcggccgaag agggttcggt cgcgggggtt tcggctgcag ggggttcggc
     2161 cgaagggggt tcggccgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggt
     2281 tcggtcgcgg gggtttcggc tgcagggggt tcggccgaag ggggttcggc cggaggtgac
     2341 tcggtcgcaa catcgggcgg cggcgttcgc tgggagtcga ccgccgccgg cagccggatc
     2401 gcgagcaccg tggcgccgat atagatgagg aaggcgaccc gcagcgacca gggcgcgccg
     2461 aaccgcccga tcggcgccgc caccgctgcg ccaagcagca tgcccatcaa cgtcgcgacc
     2521 gactgccgcg agttggctgt caccagactg atcgcgtcgg gtcgcacgag gggtatgccg
     2581 gccgagaggg ccacggcata cgcgcgggtt gccatgaggc agagcaaggc gaggggcagc
     2641 aaccagggcg acccgtccgc gaccacgccg gcgagcaccc acgagaggaa ggcccggagc
     2701 gccagcgtgg tgccgatcgc ccagcgccga ccgtgccgcc aacgatccag cagggggccg
     2761 acgaggggcg caaggacgac gaacggtgcc atcgtcgtca cgaggaacaa cgcgacctgg
     2821 ccgcgtgcct ggccgagtgg gagggccagg atcgtgctgg ccagcgccac ggtgaaggct
     2881 gcgtcgcccg ccgaggcaag gacctgcatc tcggtgaccc ggctcaggcc cgactcagcg
     2941 gcgccttccg atcgggcgaa cagccggaac cgttccgtcc cagccttcgc gccccgaccc
     3001 atgaacttgg ctccgacgac gacacccttg gccgtggcct tgctcgcccc ggcggtggct
     3061 cgcccggcgg ccttgccggc ccgcgccgtc gcccgacccg tcaccgctgc cgcccgccgg
     3121 gcccgttcgc tcgccacctc gaccagcgcc ggttggtcgg tcgcgtcgtt gcgcagcggc
     3181 tgtcgcggct ggggttcccc cgcggcgcca gtgcccgcgc cggggccggc cgcggcaccc
     3241 aagcggctgc ccggatcggc ccccgcgtcg cgcatcgctg gctccccggt gtccacgccc
     3301 ccatcctgcc gcgtggacgc cgaccgggcg gcgcggcata ccggggattc ccctcgggcg
     3361 acgcgcggtc ccggggttat gccacatctg tgccccgcct tcagtcaact gtgtgactgc
     3421 ccacgttcac ctctaggggg gcgatttgcc gggaagtggc cactatcttc ggcagagacc
     3481 tcgtgcatcg atcgcactgc cgcgaccgca gtcactccag cgcgcgaaaa cccagcaccg
     3541 agaggacccc ccaccgtgcc cgttgatcgg acatctgaag gagcccacgc cgactcaccg
     3601 tcggttgact ttcgcgacat cgggccggcg ttcccgccga cgcatccgct ggccctcagc
     3661 ccgacagtgg cacccgaacg caccgtcgac ggattcgtgc aggactacct gcgtgagctg
     3721 aacttcggcc agggcgcagc actgacgcgt tccacggtca accaccaata cctcgcgttg
     3781 gccagaactg tgcgccacca cctcatggcg gcctggctgg agaccgccca acggcgccgt
     3841 gagcacccga ccaagatggt cggctatctg tccgcggaat acctgctcgg gcggcaactc
     3901 gggaacgcac tcatggccac cgatctgcgc gacgtcgtca acgaggcgat gaaggcttgt
     3961 ggactggacc tgcgcaaggt gcgcgtccag gaggtcgagc cggggctggg caacggcgga
     4021 ctgggccgtc tcgcagcgtg cttcatcgac tcgttggcca cgatggacgt cagttgcatc
     4081 ggttacggga tccgctacga gtacgggatc ttccggcaga cctttgtcga cggacgccag
     4141 gccgaggtcc ctgacaactg gctgtccctc ggggcgccat gggagttccc caaccccgag
     4201 cgggccgtgc gcgtcgactt cggtggccgg gtcgaggacg tcgtcgatga ggcgggcgtg
     4261 acccgccgcc agtgggtgcc agcctggaat gtgctgggaa tcccttacaa ctacatggtt
     4321 cccggctacc gcaacggcgt cgtcaacacc ctgcggctgt ggagcgccga ggcgacccgg
     4381 gcgttcgacc tcacgatctt caactccggc gactacgccc aggccgtgcg cgcccagacc
     4441 ttcgccgaga acatctccaa ggtgctctac ccggaggact cgactcccca ggggcgggag
     4501 ttgcgcctgc agcagcagta ctttttcgtc gcctgttcgc tgcgggacta cctcgacggg
     4561 accttgcccc agggcttcga cctgcgcaaa ctccccgagc agatcatctt ccagctcaac
     4621 gacacccacc cggtcatcgc catccccgag ttcatgcgcc tgctcgtcga cgagaagggc
     4681 atggagtggg acgaggcctg ggcgatcacc cagaagtgct tcgcctacac gtgccacacg
     4741 ttgctgcccg aggcgctcga ggtgtggccg accgatctgc tcgggcggct gctcccccgg
     4801 catttggaga tcatctaccg gatcaacgag gagttcctcg cgagtgtgcg cgcggcatac
     4861 ccgggcgacg agatgcgcgc gcggcggatg tcgatcatct ccgactaccc gcaggcggtg
     4921 cggatggcac acctcgcgac ggtcacggcg gtccgcgtca acggcgtcgc cgaactgcac
     4981 tcccagttgc tgcgtgacaa ggtgctgccg gacttctcgg cgttctggcc ggagaagttc
     5041 accaacgtca cgaacggcat caccccgcgt cgcttcatcc gaatggccaa tccggctctc
     5101 tcgcaggtga tcaccgacgc catcggagag gggtgggtca ccgacctcga gcggttgcgt
     5161 gagctggagc cgtatgtcga tgacccggct ttccgtgccg agttccgtcg cgcgaaggcc
     5221 gccaacaaga agcggctcta cacgttggcc gaggcgttcg agggtgtcag cctgccggaa
     5281 gggcacctca tcgacgtcat ggtcaagcgg ctgcacgaat acaagcggca gttgctcaag
     5341 gtgctgcacg tcgtctcgac gtacgagggc atcctgtcgg gggccgtgcg tgcggaggac
     5401 atcacgcccc gcctcgtcgt gttcggcgcc aaggcggctc cgggctactt catggccaag
     5461 gagatcatct atctcatcaa ccggatggcc accgtcatca acaatgaccc tctggtcaag
     5521 cggcagttga tcgtcggctt cccggccaac tacaacgtca ccctcgccga gaagatcatc
     5581 ccggcggccg acctgtccga gcagatctcc ctggccggca aggaggcctc cggcaccggc
     5641 aacatgaagt tcaccctcaa cggggcgctg accatcggca ccgacgacgg cgccaacgtc
     5701 gagatccggc gcctcgtcgg cgatgacaac ttcttcctct tcgggatgcg cgagcccgag
     5761 gtggtcgcgt tgcaggccaa gggctatgtg ccgagttcct actacgagag caacccgacg
     5821 ctgcgacggg cgatcgacct catcgcgagc ggccacttca ccgagggcaa ccgcgacgcg
     5881 gtcgggccga tcatcggaga cctgctgagc aacgaccgct tcctcgtgct cgccgactac
     5941 cagtcctaca tcgacgcgca ggctcgggtg gacacggcat accgggacga agaggggtgg
     6001 acccgatcgg cgatcctcaa cgtcgcccgg gccggcttct tctcctccga ccgctcgatg
     6061 cgcgactaca tcgaccgggt gtgggggctg cggcagtcgt gagagggtgg ccgcaccagc
     6121 cggcggttcc acgacaggag cgctcatgag tgcggtcatc cacttcgaga tccatgccaa
     6181 cgacgtgcct cgcgccgtcg ccttctacca ggccatcttc ggctgggagg tccaggactg
     6241 gtcggagtat gccgggatgc cctacttcgg ggtcgtgacc cgccccgagg gggaacccgg
     6301 catcaacggc gcgatcatgg cgaggatggg cccaggcccc cagccgggtg gaccggtcgg
     6361 cggcgctgtc ctgaccgtcg gttgtgagga cttcgacggc accgccgaga aggtcctgca
     6421 ggctggcggg acggtcgcgc tggccaagca cgcgttgccc gggatggcgt ggcagggcta
     6481 tttcctcgac accgagggga acgtcttcgg ggtgcatcag cccgacgtcg acgcccagta
     6541 gtcaggaagg tgcgcatgtc tgaactggtc gaccttctcg gccaacggcg agacgcggcg
     6601 gaggtcgtcg aggcggttcg gcagttcggg ttggacgagg tgatggatga cccgccatcg
     6661 tgtcagtatg tgatgtcgcg gcgctgacgg ggcggtcaaa ctggtcgtga cctttgacga
     6721 gacctcgacc ctgaagtacg tgagcctgga gacgctgtag ctagatctgc ggcgtctcga
     6781 cggggatgcc gaatcgaccg aagtcacgat cgaaggtcac gaccgtggcc gatgactcga
     6841 tggccagcgc ggccagatgc gcatcggtcg tcagattgcc ggcggtcccg gactcccgca
     6901 gcaggccggc tagcagcgac aggtgccgag ccgatggttc gggaatgacc acggctgggg
     6961 ccgacagcca ggtctggacc agctcggtcg cctgagcggc cgtaaggggg cacgggaaaa
     7021 cccgcgggtg ggtcaagatc cggatgaacc cgagcagggt gacccagggc atcccgatca
     7081 cctcgggccc atgcagagcc tgcgtgagcc agccacgcgc actggcgtga tgcgcggagc
     7141 gctcgctcac ggcatacaga agaacgttgg tgtcgacgat cttcatcggc cgcgcgcgag
     7201 gtcctggccg atctcgtcgg cctccaactc cgcgacgaaa gcggcggcat gggagaggtc
     7261 gacgaacggc tcacccatgt cgaacgtcgg gaaacgcagg tccagcgcag gagcggcggg
     7321 cgcgagcccc gcgcggatgg ccgagttgag cgcctccttg aacgacacgc cgcgctcacg
     7381 catggcccga tgcaccaacg cctccacgtc aggctccagc gtcaccgttg tccgcgacat
     7441 gggacgcatc atagcatcat gtccaatgat gctgtgatgc aaaagaagcg aagcgttggg
     7501 ccctcggggg agcaagaccg acatcaggac gtcggaatcg ctcacccgag ggccgtcgac
     7561 accgctggtc agacaccacc cgtcagacga gcgtgccgaa cttgccgttg ttgaagtcga
     7621 ccatcgcctc gcggatctcc tccatcgtgt tcatcacgaa cggaccgtag aacacgaccg
     7681 gctcgtcgat cggcttgccg ccgagcacga ggaaccgagc gcccacctcg ccggcagtga
     7741 cctgcagacg gtcaccctcg cggttgaaga tgcccatctc ctcacggccg atcgtccctg
     7801 tcgacgtcgt cagctcaccg tcgagcacga acgtcatcag cgtgtgaccg tcgggcacgg
     7861 caaggtcggc cgactcgtcg gcaccgagtt gcacatccca cagcgagatc ggggtgacgg
     7921 tgcgagcggg cccgcgcacg tcgtcgagct caccggcaac gagggtcacc acgccaccgt
     7981 cgtcgagctt gaccttgccc atcgactcgg cggtcagcgg ctggtaaccc ggcgcgtcca
     8041 tcttgtgcgc cgcaggcagg ttgacccaca gctgcatcat gtggaaacgc ccaccgcggg
     8101 ccgcccactc cggctcgtga tactccttgt ggagtatgcc gctcgcggcc gtcatccact
     8161 ggacgtcacc cgggcggatg acgccaccgg cgccggtcga gtcgtggtgg gcgacagccc
     8221 cctcgaaggc catcgtcacc gtctcgaagc cccggtgcgg gtgcacgccg accccacggg
     8281 gagtcgtggt cggcgggtac tccttgaccg ggttgtagtc catgagcagg aacgggtcgg
     8341 tgagccgctg cagctcgggc gacgccggga gcaactgggt cacatagaac ccgtcgccga
     8401 cccagtggaa tccgtcgcca cggtggacgg tgtgcagagt gcggatggtc atgacagaaa
     8461 cctctcagag aagcagttgt tggtcggggg attcagcgtg gttttggggg cgggttagac
     8521 ggtgaagccg agggcctcgg cgcgacgcag ccagcccttg ccctcgtcgc cccgcgcctg
     8581 ccggcacagg acgcgcccga ggaggagcac ggcatacgga tcgctcggat cggcggcgac
     8641 caactcgcga gccgcacgct ccgcgcggcc cagttgggcc gagtggtagt aggcgcgggc
     8701 cagcagcagg cgcgcctccc cggtgccggt ctgctcgggc agatcctgca gatcctggag
     8761 caactcctcg aggaggagag cggcctgggc atagcgacgc tgcgagaaga gcagctcggc
     8821 ggtgcggtaa cggttgtaga ggtcagtgcg gtccatgcgg tcgtccatgg cactatcaac
     8881 atcatagtga ctcggatatt cccaggcact atgagactcg gggtagactg atgcccaaca
     8941 cccgaaagga gccgtcatgc ccgacgctgt gcccgaagcc gagcccgaag ccgagcccga
     9001 acccggcacc cctgagtgca cggtggtcga gagcgtcctg gccttcctcg gccgcgcgtg
     9061 ggcaggcgcc gtcgtcg
//
LOCUS       DAOMZY010000125         9028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_7292_length_9028_cov_5.498830, whole genome shotgun sequence.
ACCESSION   DAOMZY010000125 DAOMZY010000000
VERSION     DAOMZY010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 9028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9028
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7292_length_9028_cov_5.498830"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..547
                     /locus_tag="PLK64_08445"
     CDS             104..547
                     /locus_tag="PLK64_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79815.1"
                     /translation="MSTKKRIAALAAVAASAALSVAMASPASANTSSGCTVTPLKPIF
                     AGFNSSGVKLVNYRISVSCTSGRTVSIDQQRYEEDPWPNGDDFLGSTSFSTSGVTTLS
                     NVRTLVDGELGDEEVYHNVRFRVSSNGVTSPWTSYQKSSVLTIGN"
     gene            complement(770..1453)
                     /locus_tag="PLK64_08450"
     CDS             complement(770..1453)
                     /locus_tag="PLK64_08450"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HPV79816.1"
                     /translation="MASHWSLPATRALTAYLMLAVANLVAIAVGASLLSNWTQWLLMP
                     ILTIAFVALARGATGLDPRLRTATLAALGFSWLGDLLPHLAPDSVAFLVMVGCFLLAQ
                     VAYIVGFLPHWRRSVLVTARPLAALYAVVFIALIAACAGGAGPLLGPVVVYGLTLTAM
                     AVLATGLGRLTGLGGALFMLSDSLIALKAFREWDGRALSMAVMGTYAAAEALLVMGIV
                     RQTAPPVTH"
     gene            complement(1747..2004)
                     /locus_tag="PLK64_08455"
     CDS             complement(1747..2004)
                     /locus_tag="PLK64_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HPV79817.1"
                     /translation="MRLVFDESAWEDYLWWQQQDRKVLRRINLLIQDVARHGNEGLGK
                     PEPLKHGFHGYWSRRITDEHRLIYKLVDDEVRIAACRYHYE"
     gene            complement(2001..2252)
                     /locus_tag="PLK64_08460"
     CDS             complement(2001..2252)
                     /locus_tag="PLK64_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HPV79818.1"
                     /translation="MRTMSYTESRARYAETLDSVVNDREEVVITRAGHEPVVIVSLED
                     YQALKETAYLLRNPANARRLLASIERLEAGGGQARELAE"
     gene            2325..3143
                     /locus_tag="PLK64_08465"
     CDS             2325..3143
                     /locus_tag="PLK64_08465"
                     /inference="COORDINATES: protein motif:HMM:NF020416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase/BAAT C-terminal
                     domain-containing protein"
                     /protein_id="HPV79819.1"
                     /translation="MRRIDLSGVEGERWVPETLSGVGALVLAGSSGRIDSARAELLAR
                     QGVIAESIRWFGGPGQHVGPWEIPLELFIGRIAELKRDCDRVLVLGTSFGAEAALLTG
                     CHTSEVDAVVGFAPSDVAWAGVRPDGTQTSHWTLGGAPLPFVPLDEDWVSGDDVPAFV
                     GLYEASRKRSPELVAEAAIAVEDIKEVVLVAGGDDQVWPALAMAAAIESRRTTHGLTT
                     TLVSDPDAGHRTILPGEPAVTAGIRMRRGGTENADRNLGTAAWAQIQALLRAGR"
     gene            3311..4168
                     /locus_tag="PLK64_08470"
     CDS             3311..4168
                     /locus_tag="PLK64_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79820.1"
                     /translation="MDSDHSIAVRNTYRYLRLAMIAVVLMLFLAVGIERLSSTGWQTS
                     ISAYYFTAVHAVFIAALCTIGACLIVYQGNTDTEEVVLNFSGFLAFVVAFVPTQREPL
                     YGPGLPATYEVGMGIRNNVLALIITGVVVEIARIIINRNVDRRPLSPWAKRATLIGWA
                     VIGVGILGYAAFPANFEAKGHTVAAVTMFVGIIAVIVLNALSAQSAQTGPSYVGSYKL
                     IAGLMALLVLAIVVIRLTLPEFAHIVIWIEGVLILAFAAFWLVQTKELWDVVDRRDLA
                     PNTTDPTGR"
     gene            4258..5436
                     /locus_tag="PLK64_08475"
     CDS             4258..5436
                     /locus_tag="PLK64_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009358666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 76 protein"
                     /protein_id="HPV79821.1"
                     /translation="MNQPGPSSAAAAPTASPAERAAYAAASVTTLFGRRMLGVPGTFL
                     PLVTSPGPRRWRGPWHYWWLAHYLDCLVDQALREHAAGDLAGARATTATARRLLRTIR
                     IRNVAIFTNHYYDDMAWLLLAVHRLDRLTARLSPGTSSALTHSAGRALRAAVTRGHTD
                     DLDGGLFWNDHHDFKNVAATGPAALFFARIGDRARARSLLDWLHDHLRDPATGLFVDG
                     LRIAPDGTTTLVPDVFTYNQGTVLAALVTLGDATSLDRAETLVSAIAAHLTTDPDQRV
                     LRTHGDGDGGLFTGILARYLALAAHSPALADGARQVARELVRGTAEAFWSGRQLRSAP
                     TTSGMPSAPATALPSVAVFSPDPTVSAEQTQPRGIPLELSTQLQAWMTLEAAATLATA
                     "
     gene            5480..5968
                     /locus_tag="PLK64_08480"
     CDS             5480..5968
                     /locus_tag="PLK64_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79822.1"
                     /translation="MDIGTFYSLFSATCFTLVGLWWNVVERRSGWAADPRRRRLAGGI
                     YVTFLLPGLMGLLAQVSPATPLMWRSTFAVVALIGVVTTVRLIGIERAFTLPGPFTRN
                     RWVVAVLYLAVLVLGAFPTVAAPLGAAPIQVAAVLLISLVVVGHGLTWEFMTDSSDAS
                     AD"
     gene            6091..7485
                     /locus_tag="PLK64_08485"
     CDS             6091..7485
                     /locus_tag="PLK64_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV79823.1"
                     /translation="MSTAGGAAATRAPKSAETREQKSWYWYDWANSAYVTTTATVLFA
                     PYLTSVATAAACPDLADGQRCAATLSVLGIPVSPGSLVAYTATVSTIISAIFLIFVGA
                     IADRSPHPTKLFATFAWTGALSATLMCLVTGTNWQMGVLLFVIANICLGSSLVIYDSL
                     LVRIAGPNDRDRVSSKGWAFGYLGGGLLLMLNFVLVAKPDLVGLDKGGAVRVSLLMAG
                     VWWAAFTIIPVRGLRNVTGTVAPPVAPGEGVVGGSLRQLRETFRDLALHYPQTKLFLL
                     AYLFFNDGIQTVIGNSSLYGIEELEFPQETVLGIFLFVQFVAFFGARFFGQVAARIGA
                     WKTVLSSLGVWTGIVTVAFFVPAKSLVLFLALAFFIGMVLGGSQALSRSLYSQLIPAG
                     KEAEYFSLYQAMERGTSWFGTLIFGLVYQATLSYRAAILALIVFFILGGVLLSRVKMR
                     EGIIQAGNEVPRVI"
     gene            7625..7981
                     /locus_tag="PLK64_08490"
     CDS             7625..7981
                     /locus_tag="PLK64_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HPV79824.1"
                     /translation="MADRSLRGTRLGALSMESDVNVVPSARQITVYVCPQGHRSELPF
                     SIEADIPPTWECRCGASARLLNGESVPDAKPARPPRTHWDMLLERRSIPELEELLAER
                     LALLRASRGETSKRSA"
     gene            complement(8081..8575)
                     /locus_tag="PLK64_08495"
     CDS             complement(8081..8575)
                     /locus_tag="PLK64_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HPV79825.1"
                     /translation="MAQRVTRSGGRRRWPWVLTLALLVVPTAELAIILWVGQQIGGWP
                     TLGLLVAESALGAWLVKREGSRTWQALASALNTGRMPSRQLSDAALVLVGGTLLLTPG
                     FLTDLVGFFCILPITRAIARTVLETIVTRKLLGGIVSSGDRPPRSPGGSGPGDVIEGE
                     IIDG"
     gene            complement(8560..>9028)
                     /locus_tag="PLK64_08500"
     CDS             complement(8560..>9028)
                     /locus_tag="PLK64_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dolichol-phosphate mannosyltransferase"
                     /protein_id="HPV79826.1"
                     /translation="LPSLLRAAENADLVIGARWIKGGSVVNWPIHRKVLSVGANIYTK
                     VMLGLPVNDATGGYRVYRTSALQTMDLGSVSSAGYGFQVDMTVALVRAGLVAVEVPIE
                     FVEREIGESKMSGNIVSEAFVNVGKQGLAHRAGQLRGAAARLRSPREGRWHSA"
BASE COUNT         1426 a         3265 c         2827 g         1510 t
ORIGIN      
        1 cggcccgaat tcctcactct cgcggtgtat ctcaggggtc gaaggaagcc tcttgacgtc
       61 catcggggcc acccagccct cccgactaga aggagttcca ctcatgtcca ccaagaagag
      121 gattgccgcc ctggccgccg ttgcggcctc cgctgccctg tcggtcgcga tggcatcacc
      181 ggcctccgcc aacaccagca gcggatgcac cgtcaccccg ctgaagccga tcttcgccgg
      241 tttcaacagc tcgggcgtca agctcgtcaa ctaccggatc agcgtgtcgt gcacctccgg
      301 acgcaccgtc agcatcgacc agcagcgcta tgaggaggac ccgtggccga acggcgacga
      361 cttcctgggc agcacgtcgt tcagcacctc gggcgtcacc acgttgtcga acgtccggac
      421 cctcgtggac ggcgagctcg gcgacgaaga ggtctaccac aacgtgcgct tccgggtgtc
      481 gagcaacggc gtgacctcgc cgtggacgtc ctaccagaag agttctgtcc tcaccatcgg
      541 caactgatgg cggcagcgcc gtgcacgggc cgggcaattc gctcggcccg tgccccgttg
      601 atagagggca ttcccgcctc gccctatagc cacttcttct cccgaatggc tagtcatccc
      661 gaatacctca cacccgtcgg gagctttcgt gcgggcggcc tctgaatgac ccaaagtcct
      721 cacatccgca caagacttcg aggaagcccg ggtatcgatc aggccatgtt cagtgagtga
      781 ccgggggagc agtttggcgg acgatgccca tcacgaggag tgcctcggcg gcggcatacg
      841 tgcccatcac agccatcgac agcgcccggc cgtcccactc gcggaaggct ttgagggcga
      901 tgagtgaatc cgagagcatg aacagcgccc cgccgagccc cgtcagccgc cccagcccgg
      961 tcgcgagcac cgccatggcg gtcagggtga ggccgtagac gacgaccggc cccaacagcg
     1021 gtccagcgcc gcccgcgcag gcggcgatga gcgcgatgaa caccacggca tacagggcag
     1081 cgaggggtcg cgcggtgacc aggacgctgc gacgccagtg ggggaggaag ccgacgatgt
     1141 aggccacctg cgcaagcagg aaacacccga ccatcaccaa gaacgcgacg ctgtccggcg
     1201 ccagatgggg gagtaggtca ccgagccagg agaagcccag ggcggccagg gtggctgtcc
     1261 gcagccgtgg gtcgagcccg gttgcgccgc gcgccagggc aacgaacgcg atggtgagga
     1321 tcggcatgag cagccactgg gtccagttgg acagcaggct tgcgccgaca gcgatcgcca
     1381 cgagattggc caccgcgagc atgaggtatg ccgtgagcgc gcgggtcgcg ggtagggacc
     1441 agtgggacgc catgaggaca tcatgcctgc ggtaaaccga ccgacgatga atctcctcac
     1501 cgccctcgct gggaaccaca cgctggcgac gcggaacgtg aacgacttcg acggcatacc
     1561 cggcctgaac gttgtcaacc cgttcgagtg agtggcgcgc cctgccatgc aagggggaga
     1621 ctcctcccgg tccaccagaa tcagtgttgc gacataatgt cgattatcgg cgttccgcag
     1681 gagggttggc ggagtgttgg ccacaccgga gtgtcgccat cactccgcaa agcgccgtcc
     1741 gcaccctcat tcgtagtggt accggcacgc ggcgatgcgt acctcgtcgt caaccaactt
     1801 gtagatgagc cggtgctcgt cagtgatccg gcgcgaccaa tagccgtgga agccgtgctt
     1861 gagcggctcc ggtttgccca gcccctcgtt cccgtggcgc gcgacgtcct ggatcagcag
     1921 gttgatccgc cgcaacacct tgcggtcctg ttgctgccac cacaggtagt cctcccacgc
     1981 cgactcgtcg aagacgagcc tcattcggcg agctcgcgcg cctgaccgcc gccggcctca
     2041 agccgttcga tcgacgccag caggcgccgg gcgttggccg ggttgcgcag caggtatgcc
     2101 gtctccttga gcgcctggta atcctccagc gagacgatga cgacgggttc atgcccggct
     2161 cgcgtgatga cgacctcttc acgatcgttg acgacggagt cgagcgtctc ggcatacctc
     2221 gcccgcgact cggtgtacga catggtccgc atgactgcct ccttccgtac ataatactgt
     2281 acgtgcgcga tgtagtgtcg cgtcgcacat gaaagggtcc agccatgcgg cgaatcgatc
     2341 tctcgggcgt ggagggtgaa cgttgggtcc ctgagaccct cagcggcgtc ggagccctgg
     2401 tgctcgcggg ctcaagcgga cgaatcgact cggcccgcgc cgagctgctg gcacggcagg
     2461 gcgtgatcgc cgagtccatc cgctggttcg gcggcccagg ccagcacgtc gggccgtggg
     2521 agattccact ggaactgttc atagggcgta tcgcagagct caagcgagat tgtgaccgcg
     2581 tgctggtctt ggggacctcg ttcggggccg aggccgcgct cctgaccggc tgccacacct
     2641 cagaggtcga tgcagtggtg ggattcgccc catcagacgt cgcctgggcc ggcgtccggc
     2701 cggacggcac gcagacctcg cattggacgc tcggcggcgc accgcttccg tttgtcccct
     2761 tggacgagga ttgggtgtca ggcgacgacg taccggcctt cgtcggcctc tatgaggcca
     2821 gtcggaagcg gtcccccgag cttgtggctg aggccgccat cgccgtcgaa gacatcaaag
     2881 aagtggtgct tgtcgctggt ggcgacgacc aggtgtggcc cgccctcgct atggccgccg
     2941 cgatcgaatc tcggaggacc acgcatggcc tcaccaccac gctcgtgtca gacccagatg
     3001 ccggtcatcg cacgattctc cccggcgaac ccgccgtgac cgcaggaatc cgcatgcgac
     3061 gtggcggcac tgagaacgcc gatcgcaacc ttggtaccgc ggcctgggcg cagatacagg
     3121 cccttctgcg cgctggtcgt tgacgacgga atccagcgtc tcggcatacc tcgcccgcga
     3181 ctcggtgaac gacatggtcc gcatctgcga cctctcacgt gtgaataccg cgccgcgaca
     3241 gccgcgttgc gggacactca gcgggtacct gccctgcctc tcccccgcac cacccccgga
     3301 agggctcacg atggactctg atcacagcat cgccgtccga aacacctacc gctacctgcg
     3361 cctagccatg atcgccgtcg tcctcatgct cttcctcgcc gtcggcattg aacggctgtc
     3421 gtccaccggc tggcagacct cgatcagcgc ctactacttc accgcggtcc atgccgtctt
     3481 catcgcggcg ctctgcacga tcggcgcctg cctcatcgtc taccagggca acaccgacac
     3541 ggaggaggtc gtgctcaact tctccggctt cctcgcgttc gtcgtggctt tcgtccccac
     3601 tcagcgcgag ccgctctacg gccccggcct acctgcgaca tacgaagtcg gcatggggat
     3661 ccgcaacaac gtcctggcac tcatcatcac gggcgtcgtc gtcgagatcg cccgcatcat
     3721 catcaaccgg aacgtcgatc gccggccgct cagcccctgg gccaagcgag cgaccctcat
     3781 cgggtgggcg gtcatcggcg tcggcatcct cggctacgcc gccttccccg ccaacttcga
     3841 ggccaagggc cacacggtgg ctgcagtgac gatgttcgtc ggcatcatcg ccgtcatcgt
     3901 cctcaatgcc ctgtcggcgc agagcgcaca gaccggcccg agctacgtcg gcagctacaa
     3961 gctcatcgcc gggctcatgg cgctcctcgt gctggccatc gtcgtcatcc gcctcaccct
     4021 gcccgagttc gcccacatcg tcatctggat cgaaggcgtc ctcatcctcg ccttcgcggc
     4081 cttctggctg gtgcagacca aggagctgtg ggacgtcgtc gaccggcgcg acctcgcccc
     4141 caacaccact gaccccaccg gccgctgaac cgtccccacg gggagccggc acgacccgag
     4201 tcagtatgcc gcgcgtcccc acgccgccca cctcctgacc cctggcagga tgaccgcgtg
     4261 aaccagccgg gaccctccag cgccgcagcg gcgccaacgg catccccggc cgagcgggcg
     4321 gcatacgcgg cggcctcggt gaccaccctc ttcgggcgac ggatgctcgg ggtccccggc
     4381 acgtttctcc ccctcgtgac ctcccccggc cccagaaggt ggcgcggccc gtggcactac
     4441 tggtggctcg cgcactacct cgactgcctc gtcgaccagg ccctccggga gcacgcggcc
     4501 ggtgatctcg ccggtgcccg agccaccacg gccaccgcgc ggcgactgct gcgcacgatc
     4561 cgcatccgca acgtcgcgat cttcaccaac cactactacg acgacatggc ctggctgctg
     4621 ctggccgtgc atcgcctcga ccgactcacg gcccggctct cccccggcac ctcgtccgcg
     4681 ctgacgcaca gtgccggccg cgccctgcga gccgcggtca cccggggcca caccgacgac
     4741 ctcgacggcg gactgttctg gaacgatcac cacgacttca agaacgttgc cgcgacgggc
     4801 ccggcggcgc tcttcttcgc ccggatcggt gatcgcgcgc gggcgcggtc cctgctcgac
     4861 tggctgcacg accacctgcg cgacccggcg acaggcctgt tcgtcgacgg cctacgcatc
     4921 gccccggacg gcaccaccac cctcgtgccc gacgtgttca cctacaacca gggcaccgtc
     4981 ctcgccgcgc tcgtcaccct cggcgacgcc accagcctgg accgggccga gacgctcgtg
     5041 agcgcgatcg ccgcccacct gacgaccgac cccgaccagc gggtgctgcg cacgcacggc
     5101 gacggtgacg gcggtttgtt caccggcatc ctggcccgct acctcgccct cgcggcccac
     5161 tcccccgccc tcgccgacgg tgcccggcag gtggctcgcg agctcgtgcg cggcaccgcc
     5221 gaggcgttct ggtcaggtcg ccaattacgc tccgcgccaa cgacatccgg tatgccgtcc
     5281 gcccccgcga cggcgctccc ctcggtcgcg gtgttctccc ccgacccaac ggtgtcggcc
     5341 gagcagaccc agccgcgcgg catacctctg gagttgtcga cccaactgca ggcctggatg
     5401 accctcgaag cggctgcgac cctggcgact gcgtgatgga tcggccgcgc cggcccggtc
     5461 tgcggcgcta ccttggccca tggacatcgg caccttctac tcgttgttct cggccacgtg
     5521 cttcaccctc gtcggtttgt ggtggaacgt cgttgagcga cgcagcggat gggccgccga
     5581 cccgcgtcga cggcgtctgg ccggcggcat ctacgtcacg ttcctgctcc cggggctgat
     5641 gggcctgctc gcccaggtga gcccagccac tcccctcatg tggcgctcga cctttgccgt
     5701 ggtggccctc atcggtgtcg tgaccaccgt gcggctcatc ggcatcgagc gagctttcac
     5761 cctccccggc ccgttcacgc gcaaccggtg ggtggtggcc gtgctctatc tcgccgtcct
     5821 ggtccttggt gccttcccca ctgtggccgc accgctgggt gccgccccga tccaagtggc
     5881 cgccgtcctg ctcatcagcc tggtcgtcgt cggccacggc ctgacgtggg agttcatgac
     5941 cgattcctct gacgcgtccg ccgactgaag ggcgcgccgt caccgggatg cccgggatga
     6001 cggagatgcc cgagatgccc gccggggcgc tcggcgtggc gcacggcata cccctgccgc
     6061 gatcccggcg ggtgtgacac gcttcacgac atgagcaccg ccgggggggc cgccgctacg
     6121 cgcgccccaa agtcagcaga gacccgtgag cagaagtcct ggtactggta cgactgggcg
     6181 aactcggcct acgtcacgac cacggcgacc gtgctctttg ccccgtatct cacctcggtc
     6241 gccacggccg ctgcctgccc cgacctcgcc gacgggcagc gatgcgccgc aacgctgtca
     6301 gtgttgggga tccccgtctc ccccgggtcg ctcgtggcct acaccgcaac cgtctcgacg
     6361 atcatctcgg ccatcttcct catcttcgtc ggagcgatcg cggaccgctc cccccacccc
     6421 acgaaactgt tcgcgacctt tgcctggacc ggcgccctct ccgcgaccct catgtgtctg
     6481 gtcacgggca ccaactggca gatgggtgtg ctgctgttcg tcatcgccaa tatctgtctg
     6541 ggctcgtcgc tggtcatcta cgactccctc ctcgtgcgca tcgccggtcc gaacgaccgc
     6601 gaccgggtgt cctcgaaagg ctgggccttc ggctatctcg gtggagggtt gctgctgatg
     6661 ctcaacttcg tcctcgtcgc caaacctgat ctggtcggac tggacaaggg tggcgcggtc
     6721 cgggtgagcc tgttgatggc cggggtgtgg tgggccgcct tcaccatcat ccccgtgcgc
     6781 gggctgcgca acgtcaccgg cacggttgcc ccaccagtgg ccccgggcga gggtgtcgtc
     6841 ggaggcagct tgcggcagtt gcgtgagacc ttccgggacc tggcgctgca ctacccccag
     6901 acgaagctgt tcctgctggc ctatctgttc ttcaacgacg ggatccagac cgtcatcggc
     6961 aactccagcc tctatgggat cgaggagctt gagttcccgc aggagacagt gctggggatc
     7021 ttcctctttg tccagttcgt cgccttcttc ggtgcccgct tcttcgggca ggttgccgcc
     7081 aggatcggtg cctggaagac cgtgctgagc agcctgggcg tctggacggg catcgtcacg
     7141 gtcgccttct tcgtccccgc caagtcgctc gtgctcttcc tggcgctggc gttcttcatc
     7201 ggcatggtcc tcggcgggtc acaggccctg tcgcgctcgc tctacagcca gctcatcccg
     7261 gccggcaagg aggccgagta cttcagcctc taccaggcga tggagcgcgg cacgagctgg
     7321 ttcggcacgc tgatcttcgg gttggtctac caagcgaccc tgtcctaccg cgccgccatc
     7381 ctcgcgctaa tcgtcttctt catcctcggc ggcgtgttgc tgtcccgcgt gaagatgcgc
     7441 gaaggcatca tccaggctgg caacgaggta cctcgcgtca tctgacgggc gttatgtcgg
     7501 gtgaatgctg acgacttcgg gaacaaaggc aaccctttgc gccgttggaa cgtctgagtt
     7561 ggtgagcagg gggctgcggc atccgaccgg acccctcccc ggctcgcccg tcaggaggtt
     7621 cagcatggcc gaccgttccc tccgaggcac ccgcctcggc gcactgagca tggagtccga
     7681 cgtcaacgtc gttccgagcg cacgacagat cacggtctac gtctgccccc aggggcaccg
     7741 ctccgagttg ccgttcagca ttgaggccga catccccccg acctgggagt gccgttgcgg
     7801 cgccagcgcg cgcctgctca acggcgagtc ggtgccggac gccaagccgg cgcgaccccc
     7861 gcgcacccac tgggacatgt tgctcgaacg tcgatccatc cccgagctcg aggagctgct
     7921 cgccgagcgc cttgccctgc tgcgcgcctc gcgcggagag accagcaagc gcagcgcctg
     7981 agtccgcacc cggcatacca aggaacccgc accttcaccc cggaaggtgc gggttctgcc
     8041 tgtccctccc caaacaaggc tgagcaaccg ccaagcgggc tcaaccgtcg atgatctcgc
     8101 cctcgatgac gtcgcccggc ccgctcccgc caggtgaccg agggggccgg tctcccgagc
     8161 tgacgatccc cccgaggagt ttgcgggtga cgatcgtctc gagcacggtg cgagcgatcg
     8221 cccgggtgat cggcaggatg cagaagaagc ccacgaggtc ggtgaggaag cccggggtga
     8281 gcagcaacgt gccgcccacc agcaccagcg ccgcgtcact gagctggcgt gagggcatcc
     8341 ggccggtgtt gagggccgac gcgagggcct gccaggtgcg ggacccttca cgtttgacca
     8401 gccacgcgcc caacgccgac tcggctacga gcaggccgag cgttggccag ccaccgatct
     8461 gctgaccgac ccagaggatg atggccaact cggccgtggg cacgacgagc agggccagtg
     8521 tgagcaccca aggccagcgg cgacggccgc cggagcgggt cacgcgctgt gccatcgacc
     8581 ctcccgcgga ctgcgcagcc gggcggccgc gccgcgcagt tgcccggccc gatgtgccag
     8641 accctgcttg ccgacgttga caaacgcctc gctgacgatg ttcccgctca tcttcgactc
     8701 accgatttcg cgctcgacga actcgatggg aacctcgacg gccacgaggc cggcccgcac
     8761 cagggccaca gtcatgtcga cctggaagcc atagcccgcc gagctgaccg aacccaggtc
     8821 catggtctgc agcgccgacg tgcgatagac ccggtaaccc ccggtcgcgt cgttgaccgg
     8881 cagcccgagc atgaccttgg tgtagatgtt cgccccgacg ctgaggacct tgcggtggat
     8941 cggccaattg acgaccgagc cgcccttgat ccaccgagcc ccgatgacaa ggtcggcgtt
     9001 ttccgccgcc cgaagcaggc tcggcagt
//
LOCUS       DAOMZY010000126         8872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_7439_length_8872_cov_6.151752, whole genome shotgun sequence.
ACCESSION   DAOMZY010000126 DAOMZY010000000
VERSION     DAOMZY010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 8872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8872
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7439_length_8872_cov_6.151752"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..556)
                     /locus_tag="PLK64_08505"
     CDS             complement(80..556)
                     /locus_tag="PLK64_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79827.1"
                     /translation="MTRLRIAAVVALAAGLTLVGAGGQGQAHADEVTVTITPTVVIVT
                     DATGRPIRTETIIPTPAANPADQPGPKTVTVTVAPSNPGVLTLGQNDAGQWIGMVIGA
                     VLGALGTVAVMTRRGRETPVGSDGSAPEPEADGSPEPDVSPDLAAEDLSPPPDPAT"
     gene            complement(553..1926)
                     /locus_tag="PLK64_08510"
     CDS             complement(553..1926)
                     /locus_tag="PLK64_08510"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HPV79828.1"
                     /translation="MIHRVGRAWLAPGAAFVLALVLRMVDLGRVKALVFDEAYYVPQA
                     WSLLNYGIERKFAKDGQSVFVSGDFAGAFTSGGDFTVHPPLGKWLIALGETAGASQPW
                     TWRISAAVAGALIAAIVAAMAIHLTRSSWWGGFGGVLLAVEGTSLVEGRTGILDTFLT
                     LFVVAGAAAALMDRDDPTPGRFRRWRLAAAVCLGLATGVKFSGAVFLVVFILLAIAWD
                     VRRRPLSAARSIGVAARTLVTISVPAAAAYTLSWAGWFLSPDGHGRDGSDGLLMNWIH
                     NQTDMLALGGAISSAHHWQSGPWAWLIQWRPTMFYVVTAPCEGTTGTCSQTITSLGTV
                     PIWWVGIVALVGLGWLLARHRDRDAAFILAFFAAGYLPWWFLTNRTIYAFYTVTIAPF
                     VILAIVLCLYRLQRSQAHRRVLAQPAALLLLAAAIAWLWLYWPVYIGIPIPQGESARL
                     LFWEGWQ"
     gene            complement(1944..2936)
                     /locus_tag="PLK64_08515"
     CDS             complement(1944..2936)
                     /locus_tag="PLK64_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79829.1"
                     /translation="MRIRHLVGAAALSLAATMATLPNAAGAPLPAPIVTQTFSSTETL
                     TATTVAGPVPQTVTHTQTMTTSGEVRTTEWVTVTTTSTAWLDATQTQTTTVYANAEPV
                     IMTATALFTAPGVTVTVPGVPVVTAETRTATDIQIGKPTETQTIAVACGTVPTVVVTP
                     TSVHTTTITPTQTAVTSTTTLKDLRSTVTETRSTTSVYTELATGDTAWETVTVSNEEG
                     GAWTMTTIITTTQTPTTTVTDVPVTLTNTVYDQVTDTVHPIGYVAVETGCPTTTTTTP
                     SAEIPTTTAPLLANTGSNSAPALVLGLLFILGGSAILALVYRRQPSSHRGSTRR"
     gene            3117..4148
                     /locus_tag="PLK64_08520"
     CDS             3117..4148
                     /locus_tag="PLK64_08520"
                     /inference="COORDINATES: protein motif:HMM:NF021599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 domain-containing protein"
                     /protein_id="HPV79830.1"
                     /translation="MVTFDAGETLAERFRSHMGHSSSLYGYAMRGMADDWEAGGPTRE
                     ACRGWEDSPNGTMVQLRFLAGVFRLVLAGAAPELVPYYPCLGGTLPPDGVWPVMREVV
                     GRHTEQVHEALAIVPQTNEPGRSTALLVGLFDAVEAWGLDRIRLIEVGASGGLNLHVD
                     RFRYDGPGWSWGPPDSPLVLHTQAPGVTPRAFEIVERLGCDVAPVDAASHEGALHLRS
                     FVWPWQLERHERLTGALAVAAAYPVVVERAGAGEFLQCVLADPPADGVLTVVWQSITR
                     MYWPATESARVAALIEDARSRMPLAHVSMEHADTPDAADAELAVDGRVLGVVHPHGIP
                     LWVRPGTHA"
     gene            complement(4168..5223)
                     /locus_tag="PLK64_08525"
     CDS             complement(4168..5223)
                     /locus_tag="PLK64_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HPV79831.1"
                     /translation="MKTATGNFFEDFTLGMRLVHATPRTVSDGDVALYTALYGPRFAP
                     TSGVTMARAMGLERMPLDSMLVFHVVFGKTVPDVSLNAVANLGYAAGRFGVPVYPGDT
                     ITAESEVIGLKANRDGKTGVVYVHSVGTNQDGATVLDYVRWVMVRKRDESAPAPETVL
                     PELPSAVPVGDLDVPWDLRGGHYDTALGGSPYLWDDYAVGERIDHVDGMTIEESEHML
                     ATRLYHNTAKVHFNQHVEAQGRFGRRIVYGGHIISIARALSYNGLANAVSIAAINGGT
                     HSNPTFAGDTVYAWSEVLERLELPGRTDMGALRLRTVATKDLACGEFPYRGDDGKYLP
                     NVVLDVDYTVLIPRRQG"
     gene            complement(5337..6188)
                     /locus_tag="PLK64_08530"
     CDS             complement(5337..6188)
                     /locus_tag="PLK64_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HPV79832.1"
                     /translation="MRHGLTILPEHPWREAAPMWRAAEDLGFDHAWTYDHLVWSGLPD
                     QTWFAAVPTLAAAATVTERIGLGTFVSSPNYRHPYTYARDILTLHDLTEGRFICGVGT
                     GGTLDSGILGETLTVRERVDRFAEYLAVLDRLLRADHVDHSGPWFTTVDARTLPGPVR
                     SSVPLVVAANGPRALRLAAAYGEGWVTTGTGGDDLDAWFQGVAALTARLDDALAATGR
                     AEGFARYLSLDASPRFALESADLYAEMVGRAGELGFTDVITHWPRPDGPYAGDVRVLE
                     TVAATLG"
     gene            complement(6373..8079)
                     /locus_tag="PLK64_08535"
     CDS             complement(6373..8079)
                     /locus_tag="PLK64_08535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HPV79833.1"
                     /translation="MSTPTALSPAYRSAAIDRLASGEELDILVIGGGVTGAGCALDSV
                     TRGLRTGLIEKRDFASGTSSRSSKLIHGGLRYLEMFDFRLVAEALKERGLLLTRLAPH
                     LVRPVPFLYPLTKPVVERPYVEAGLALYDSMARASRHSAGLPWHKHLTRRQALKIAPA
                     LKSTALVGAVQYYDAKVDDARHTMELARTASSYGAHVATRVAAVGFLRQGERVTGVRA
                     LDKVTGREFDILARQVVNATGVWTDDTQALVGERGQFHVRASKGVHLVVPRDRIHSKS
                     GIILRTEKSVLFVIPWGRHWIIGTTDTDWALDKAHPAATSADIDYLLGHVNRILRTEL
                     TQADVEGVYAGLRPLLAGESDQTSKLSREHVVAHPTPGLVVVAGGKYTTYRVMAIDAI
                     DEAARGLGGEVPPSTTEDIPLVGAVGYRAMWNRRARLAHESGLHVERIEKMLMRHGVL
                     TTEVLALVAADRSLGEPLPGGEDYLKAEIVYAASHEGALHLDDILGRRTRLSIESFDR
                     AITASRPAAELVAPILGWDAATVDEEVEHYRQRVEAERMSQTMPDDASADAARLQAPD
                     RG"
     gene            complement(8132..>8872)
                     /locus_tag="PLK64_08540"
     CDS             complement(8132..>8872)
                     /locus_tag="PLK64_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPV79834.1"
                     /translation="APGEAKNTYGTGNFMLLNTGTEPVRSKAGLLTTVAYQFAGEQAA
                     YALEGSIAVTGSAVQWLRDQLGIISGAADIERLAAQVPDNGGVYFVPAFSGLFAPYWR
                     SDARGAIVGLSRFNTNAHLARATLEAICYQTKDVADAMFADSGVRLDTLKVDGGVTAN
                     ALCMQIQADILGVQVSKPVVAETTALGAAYAAGLAVGFWRTTDELRANWQEDRRWSPQ
                     WTDEQRTTGYAAWRKAVSRTLDWAPVSG"
BASE COUNT         1336 a         2893 c         3257 g         1386 t
ORIGIN      
        1 ggttgtcggt cttgacctgt tgttcactcg tcaccgacta atgctcgcat gtcggtgacg
       61 atcggatcgc cgggtcggat caggttgccg ggtccggcgg gggcgagagg tcttcggcgg
      121 cgaggtcggg actgacgtcg ggctcgggtg acccgtcggc ctcgggctcg ggcgcgctcc
      181 cgtccgaacc gacgggcgtc tcccgacctc ggcgggtcat caccgcaacg gtgccgagcg
      241 cacccaacac ggcgccgatc accatgccga tccattgccc cgcgtcgttc tggcccaggg
      301 tcaacacgcc tgggttggat ggggcgacgg tgacggtcac cgtcttgggc ccaggctggt
      361 cggcggggtt cgcagccggc gtggggatga tggtctcggt gcggatgggc cgcccggtgg
      421 cgtcggtgac gatgacgacg gtcggggtga tcgtcacggt gacctcgtcg gcgtgggcct
      481 gaccctgccc gcctgcgccc accagggtca ggccagcggc gagggcgaca acggccgcga
      541 tgcgcaggcg cgtcattgcc agccctccca gaacagcagg cgggccgact cgccctgagg
      601 gatcgggatc ccgatgtaga cgggccagta gagccacagc caggcgatcg cggcggccag
      661 caggagcagg gcggccggtt gggcgagcac gcgccgatgg gcctggctgc gctggagtcg
      721 atagaggcac aggacgatgg caaggatgac gaacggggcg atggtcaccg tgtagaacgc
      781 gtagatcgtg cggttggtga ggaaccacca ggggagatat ccagcggcga agaacgccag
      841 gatgaaggcc gcgtcacgat cgcggtggcg ggccagcagc caccccagcc cgacgagggc
      901 cacgatgccg acccaccaga tcggcaccgt tcccagcgag gtgatggtct ggctgcaggt
      961 ccctgtcgtg ccctcgcagg gagcggtgac gacgtagaac atcgtcgggc gccactggat
     1021 cagccaggcc caggggccgg actgccagtg gtgagcagag ctgatcgcac cgccgagggc
     1081 gagcatgtcg gtctggttgt ggatccagtt catcagcagg ccgtcgctgc cgtcgcgacc
     1141 gtggccatcg ggggagagga accagcccgc ccaagacagg gtgtatgccg ccgcggccgg
     1201 gacgctgatg gtcaccaggg tgcgggcggc aacgccgatc gatctcgccg cactgagggg
     1261 acgccggcgc acgtcccagg cgatcgccaa caggatgaac accacgagga agaccgctcc
     1321 ggagaacttc actccggtcg ccagccccag gcatacggca gcggcgagtc gccagcgtcg
     1381 gaaccggcca ggggtggggt cgtcgcggtc catcagcgcc gcggccgctc ccgcaacgac
     1441 gaagagggtg aggaaggtgt cgaggatgcc ggtgcgaccc tcgacgagcg aggtgccctc
     1501 gacggcgagc agaacgccgc cgaacccacc ccaccacgag gatcgggtga ggtggatggc
     1561 catcgcggcg acgatcgctg caatgagcgc cccggccacc gcggccgaga tccgccacgt
     1621 ccagggttgg cttgctcccg cggtttcccc gagggcgatg agccatttgc cgaggggcgg
     1681 atggacggtg aagtcaccgc cggaggtgaa ggctcccgcg aagtcgccgc tcacgaagac
     1741 cgactgcccg tccttggcga acttgcgctc gatgccgtag ttcagcagcg accaggcctg
     1801 cggaacgtag taggcctcgt cgaacaccag ggccttcacc cgcccgagat cgaccatccg
     1861 caggacgagc gccaacacga aggccgcgcc cggcgcgagc caggcgcggc caacgcggtg
     1921 gatcatcacg gcaccaacgt agatcagcgg cgcgtcgagc ctcggtggga ggacggctgg
     1981 cggcggtaga ccagggccag gatcgccgag ccgcccagga tgaacagcaa gcccagcacg
     2041 agcgccggag cggagttcga gccggtgttg gccaggaggg gggcggtcgt cgtcggaatc
     2101 tcggcgctgg gcgtcgtcgt ggttgtcgtc gggcagccgg tctcgaccgc cacgtagccg
     2161 atcggatgca ccgtgtcggt cacctggtcg tagacggtgt tcgtcaacgt cacgggtacg
     2221 tcggtcaccg tcgtggtcgg agtctgagtc gtcgtgatga tcgtggtcat cgtccaggcg
     2281 cccccctcct cgttcgagac cgtcaccgtc tcccacgcgg tgtcgccagt ggccagttcg
     2341 gtgtaaacgg acgtcgtgga ccgagtctcc gtgaccgtcg accgcaggtc cttcagcgtg
     2401 gtcgtcgagg tgaccgccgt ctgcgtcggc gtgatggtcg tcgtgtgcac ggacgtaggg
     2461 gtgaccacga cggtgggcac tgtgccgcag gccacggcga tcgtctgggt ctcggtgggc
     2521 ttgccgatct ggatgtcggt ggcggtccgg gtctcggcgg tgacaactgg cacacccggc
     2581 acggtgacgg tcacgcctgg tgccgtgaag agggccgttg cggtcatgat gacgggttcg
     2641 gcgttggcat agacggtcgt ggtctgagtc tgcgtggcgt cgagccatgc cgtgctggtc
     2701 gtcgtcaccg tgacccactc ggtcgtgcgc acctcgcccg acgtggtcat cgtctgggtg
     2761 tgggtcaccg tctgagggac cggcccggct acggtcgtcg ccgtcaaagt ctcggtgctg
     2821 gagaaggtct gggtcacgat cggcgcgggg agtggagcac ccgccgcgtt gggcagggtc
     2881 gccatcgtcg ccgctaggga cagcgctgct gctcccacca agtgtcgtat gcgcatgggt
     2941 gcactcccgt gtcgtgtact cccagtccgc ccgcgacccc ccgcgtgcgg actcttctcc
     3001 cgtgagcgga tcgtaccgca catcgactaa cccatgttaa gaaagtctct ccaggacctg
     3061 ttcggaggtc ggtggaggag aagtcccggg tgacgagccc gtgacatgct gacgtgatgg
     3121 tgaccttcga cgccggggag acgctcgccg aacggttccg ttcccacatg ggtcactcgt
     3181 cgagcctcta cgggtatgcg atgcggggca tggccgacga ttgggaggca ggcggcccca
     3241 cacgtgaggc ctgtcgcggc tgggaggact ccccgaacgg cacgatggtg caactgcgct
     3301 tcctggccgg cgtcttccgc ctggtcctgg ccggtgcagc cccggagttg gtgccctact
     3361 acccgtgcct gggcgggacc ctgccgcccg acggggtctg gccggtcatg cgtgaggtgg
     3421 tggggcggca taccgagcag gtccacgagg cgctggcgat cgtcccgcag accaacgagc
     3481 cgggccgttc gactgccctg ctcgtcggcc tcttcgacgc ggtggaggcc tgggggctcg
     3541 atcggatccg gctcattgaa gtgggggcgt ccggtgggct caacctgcac gtcgaccggt
     3601 tccgctacga cgggccgggg tggtcgtggg gaccgcccga ctctccgctg gtgctgcaca
     3661 cccaggcgcc cggagtcacc ccccgagcgt tcgagatcgt cgagcgcctg gggtgcgacg
     3721 tggccccggt cgacgctgcc tcgcacgagg gagcgctgca cctgcggtcc ttcgtctggc
     3781 cgtggcaact cgagcgccac gagcgactca ccggcgcgct cgcggtggcg gcggcatacc
     3841 cggtggtggt cgaacgggca ggagcggggg agttcctgca gtgcgtgctc gccgatccac
     3901 ctgctgacgg ggtgttgacc gtcgtctggc agtcgatcac ccgaatgtat tggcccgcaa
     3961 cagaatccgc gcgggtcgcc gcactcatcg aggacgcccg atcgcgtatg ccgctcgcgc
     4021 acgtgagcat ggaacacgcc gacacccccg acgcggccga tgccgagttg gccgtcgacg
     4081 ggcgggtgct cggggtggtg cacccgcacg gcatacccct ctgggtgcgt ccggggaccc
     4141 acgcgtgacg cgcgggcaaa ctgcgggtca gccctggcgg cgcgggatga ggaccgtgta
     4201 gtcgacgtcc aacacgacgt tggggaggta cttcccgtcg tcgccgcggt aggggaactc
     4261 accgcaggcg aggtccttgg tggcgaccgt ccgcaaccgc agcgcgccca tgtcggtgcg
     4321 ccccggaagc tcgagtcgct ccagcacctc cgaccaggcg tagacggtgt ctccggcgaa
     4381 ggtcgggttg ctgtgcgtgc cgccgttgat cgctgcgatc gacaccgcgt tggccagtcc
     4441 gttgtaggac agtgcacgcg cgatgctgat gatgtggccg ccatagacga tccgccggcc
     4501 aaaccggccc tgcgcctcga cgtgctggtt gaagtggacc ttggcagtgt tgtggtagag
     4561 ccgggtcgcg agcatgtgct cggactcctc gatggtcatg ccgtcgacgt gatcgatccg
     4621 ctcgccgacc gcatagtcat cccagaggta ggggctgccg ccgagcgccg tgtcgtagtg
     4681 gccgccccgc aggtcccagg ggacgtcgag gtcgccgacc gggaccgcgc tcggcagttc
     4741 aggcaggacg gtctcgggag ccggcgccga ctcgtcgcgc ttgcggacca tgacccagcg
     4801 cacgtagtcc aggaccgtcg cgccgtcctg gttggtgccg accgagtgaa catagacgac
     4861 gccggtcttg ccgtcgcggt tggccttgag cccgatgacc tccgactcgg ccgtgatggt
     4921 gtcacccggg taaaccggca cgccgaagcg ccccgcggca tacccgaggt tggcgaccgc
     4981 gttgagcgag acgtccggca ccgtcttgcc gaagacgacg tggaacacga gcatgctgtc
     5041 cagcggcatc cgctccaacc ccatggcgcg agccatcgtc acgcccgagg tcggtgcgaa
     5101 ccgcggcccg tagagcgcgg tgtagagcgc gacatcgccg tcgctcaccg tgcgcggcgt
     5161 ggcatggacc aggcgcatgc ccagcgtgaa gtcctcgaag aagttgccgg tcgccgtctt
     5221 catctcgcct ctcctgggtg ctgctgcgca cgcggtggtg ctctggtggt gctccttcag
     5281 cgatcctccc actcgctgtg ccgagcgtgc ggccgtcgac ggtggcgtcg atcacgtcag
     5341 cccagcgtcg cggcgaccgt ctcaaggacg cgcacgtcac cggcatacgg gccgtccggg
     5401 cgaggccagt gagtgatgac atcggtgaag ccgagttcgc ctgcgcgacc taccatttcg
     5461 gcgtagagat cggcagattc cagggcgaaa cgcggggagg cgtccagcga caggtagcgc
     5521 gcgaaaccct ccgcgcgccc ggtcgccgcc aaggcgtcgt cgagtcgcgc cgtcagcgcg
     5581 gccacaccct ggaaccacgc gtccagatcg tcgcctcccg tgccggtcgt cacccagccc
     5641 tccccgtatg ccgcggcgag acgcagcgcg cgcggcccgt tggcagcgac gaccaggggg
     5701 accgacgagc ggaccgggcc aggcagggtc cgggcatcga ccgtggtgaa ccatggcccc
     5761 gagtggtcca catgatcggc ccgcaggagc cggtcgagga cggcgaggta ctcggcgaac
     5821 cggtcgaccc gctcgcgaac ggtcagcgtc tcgccgagga tgccggagtc gagggtgcct
     5881 cccgtgccga cgccgcagat gaagcggccc tcggtgaggt cgtgcagggt gaggatgtcg
     5941 cgggcgtagg tgtaggggtg gcggtagttg ggggagctga cgaaggtgcc caggccgatc
     6001 cgttcggtga ccgtagcggc cgctgccagg gtcggcacgg ccgcaaacca ggtctggtcg
     6061 ggtaggccgc tccagacgag gtggtcgtag gtccaggcgt ggtcgaagcc gaggtcctcg
     6121 gcggcccgcc acatgggcgc ggcttcgcgc caagggtgtt cgggaaggat ggtcaggccg
     6181 tggcgcatct gctcagtctg cccaaggttg aggctgcact cgtctgagtc tgcacatgtt
     6241 ggcggggccg catggtcacg gttccgactg cgacgtcggc tccgggtgcg gccccacatc
     6301 gactgtaacc gaaacgtgac cggaacgcgc gcggttgaag atgaacggag ggtgacgtgg
     6361 ctcaacccac gcttaacccc ggtcgggggc ctgcaggcga gccgcgtcgg ccgaggcgtc
     6421 gtcgggcatc gtctggctca tcctctcggc ctcgacccgt tgccggtagt gctcgacctc
     6481 ttcatcgacc gttgcggcgt cccagccgag gatcggggcg acgagctcgg cggccggccg
     6541 tgaggcggtg attgcgcggt cgaacgactc gatcgacaat cgcgtgcggc gcccgaggat
     6601 gtcgtccagg tgcagcgcgc cctcgtgcga cgcggcatac acgatctctg ccttgaggta
     6661 gtcctccccg ccggggagcg gctcgcccag cgatcggtcg gccgcgacga gagcgagcac
     6721 ctcggtcgtc aggaccccgt ggcgcatgag catcttctcg atccgctcga cgtgcaggcc
     6781 cgactcgtgg gcgagccgcg cgcgacggtt ccacatggcg cggtagccga ccgctccgac
     6841 gagggggatg tcttcggtcg tcgacggggg gacctcgccg ccgaggccgc gggccgcttc
     6901 gtcgatggcg tcgatggcca tgacgcggta ggtcgtgtat ttgccgccgg cgacgacgac
     6961 cagccctggc gtcgggtggg cgacgacgtg ctctcgggac aacttggagg tctggtcgga
     7021 ctcgccggcg agcaacggac gcagcccggc gtagacgcct tcgacgtcgg cctgggtgag
     7081 ctcggtgcgc aagatccggt tgacgtggcc gagcaggtag tcgatgtcgg ccgaggtcgc
     7141 tgccgggtgg gccttgtcca gcgcccaatc ggtgtcggtc gtcccgataa tccagtgccg
     7201 tccccacggg atgacgaaga gcaccgactt ctcggtgcgc aggatgatgc cgctcttgga
     7261 atggatgcgg tcgcgcggga cgacgaggtg cacgcccttg gatgcccgca cgtggaactg
     7321 gccgcgctcg ccgaccagcg cttgggtgtc atcggtccac acgccagtcg cgttgacgac
     7381 ctggcgggcc aggatgtcga actcgcggcc ggtcaccttg tcgagcgccc gaacccccgt
     7441 aacccgctcg ccctgacgca aaaaccctac tgccgcaacg cgggtcgcga cgtgggcgcc
     7501 gtaggacgat gccgtgcgag cgagctccat ggtatgccgc gcgtcgtcca ccttcgcgtc
     7561 gtagtactgc acggcaccga ccagcgcggt cgacttgagg gccggggcga tcttgagcgc
     7621 ctggcgcctg gtgaggtgct tgtgccaggg cagcccggcc gagtgccgcg acgcccgggc
     7681 catcgagtcg tagagcgcca ggccggcctc gacgtagggc cgctcgacca ccggtttggt
     7741 cagcgggtag aggaacggca ccggtcgcac caggtgaggg gcgaggcggg tgagcagcag
     7801 cccgcgctcc ttgagcgcct cggcgacgag ccggaagtcg aacatctcca ggtagcgcaa
     7861 gccgccgtgg atgagcttgc tgctgcggct ggacgtgccc gaggcgaagt cgcgcttctc
     7921 gatgagcccc gtgcgcaggc cccgggtcac cgagtccagg gcgcagccgg cgcccgtcac
     7981 cccaccgccg atgacgagga tgtcgagttc ctcgccggag gcgaggcggt cgatggcggc
     8041 tgaacgataa gcgggggaca gcgcggttgg agtgctcacc gtcccatcct cgcaaatctc
     8101 tacgccggcc ggaccggtgc cccgggtccc gtcagccgga gacgggcgcc cagtcgagag
     8161 tgcgggagac ggccttgcgc cacgcggcat acccggtggt gcgttgctcg tcggtccact
     8221 gcggcgacca gcgacggtcc tcctgccagt tggcgcgcaa ctcgtcggtc gtgcgccaga
     8281 aaccgacggc cagaccggcg gcgtaggcgg cccccagcgc ggtcgtctcg gcgaccaccg
     8341 gcttgctcac ctggacgcca aggatgtcgg cctggatctg catgcacagg gcgttggccg
     8401 tgacgccccc gtcgaccttg agggtgtcca ggcggacacc ggagtcggcg aacatcgcgt
     8461 cggccacgtc cttggtctgg tagcagatcg cctcgagggt cgcgcgggcc aggtgtgcgt
     8521 tggtgttgaa gcgcgagagc ccgacgatcg cgccgcgggc gtccgagcgc cagtagggag
     8581 cgaagaggcc ggagaaggcg gggacgaaat agaccccgcc gttgtcgggc acctgcgcgg
     8641 cgaggcgctc gatgtcggcg gctccgctga tgatgccgag ttggtcgcgc agccactgca
     8701 cggccgatcc ggtcacggcg atggagcctt ccagcgcgta tgccgcctgc tcgccggcga
     8761 actggtaggc cacggtcgtc agcagtccgg ctttcgagcg gaccggctcg gtgcccgtgt
     8821 tgaggagcat gaaattgcct gtgccgtaag tgtttttggc ctcgccggga gc
//
LOCUS       DAOMZY010000127         8866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_7445_length_8866_cov_5.901827, whole genome shotgun sequence.
ACCESSION   DAOMZY010000127 DAOMZY010000000
VERSION     DAOMZY010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 8866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8866
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7445_length_8866_cov_5.901827"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PLK64_08545"
     CDS             <1..617
                     /locus_tag="PLK64_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPV79835.1"
                     /translation="YVGYEEGGQLTEAVRRKPYSVILFDEIEKAHPDVFNVLLQVLDD
                     GRLTDGQGRTVDFRNVILVMTSNIGSQFLVDPMLTDEAKREAVMGMVRASFKPEFLNR
                     LDEVVIFDPLSTEELARIVELQVRALSARLADRRITLEVTEAAKEWLAVTGYDPAYGA
                     RPLRRLVQREIGDRLARALLAGEVRDGSTVTVDRDDEADSLILR"
     gene            725..1960
                     /locus_tag="PLK64_08550"
     CDS             725..1960
                     /locus_tag="PLK64_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPV79836.1"
                     /translation="MTNPLIASLAKAVEAAPTDVALRLHLAELLLADGQLDAALGHCA
                     TALQQDPASGPARELMGRALGGIPQPPTGPPAEATPADSGQAHGFDWSAAESEVKDIA
                     PEPMFVEGDAGAEPEVGAWDVERATVRLADVGGMREVKERILVSFLAPMQNPEMRKLY
                     GKSLRGGILLYGPPGCGKTFIGRAIAGEMGAQFINVGLSDVLDMYIGQSERNLHELFQ
                     IARRNAPVVIFLDEVDALGQKRSLTRHSAMRGTVNQLLAELDGVSDSNEGVYVVGATN
                     QPWDVDPALRRPGRFDRTLLVLPPDQEAREAILGYHLKDRPIEGINLASLAKATDGYS
                     GADLAHICESAAERAMMEGIRSGAVRMIRMDDLYAALREVRPSTGPWFDAARNVVQFA
                     NTDGTYDELRTYMKRTKKL"
     gene            1967..3067
                     /locus_tag="PLK64_08555"
     CDS             1967..3067
                     /locus_tag="PLK64_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPV79837.1"
                     /translation="MARNVTPDPSERVRTYLLLGRDAEALAACSRALTHNPTDTGLLT
                     LSSIASYRLGQHDDAIRFAMMAISSGPDSDSAHRALATALLTVGRAQEATSAAYTAAA
                     LAPHNWVNHLRYSQCLMAMPDSRDAAWQAANRAVELAPQESLAHYQVACVVYPVGPST
                     RAQLDLAERAMREALRLEPSNATYQHELARLKLVRSGVDGVGGGAASEVLAGFTGAVL
                     ADPIGVGKGALENIRLVLRRLVLVQSQMVMAAACVGILMLGGLPSRPGKVVLDSSSAL
                     RGKLGLLAVVVALCIYFVVAWRRNLVGPSQQNVIRQVVRGDRRLSVGAGLLAAALPVM
                     AAGAFLPGDGPAMAAAGTFLLASAAAILARAK"
     gene            complement(3166..5271)
                     /locus_tag="PLK64_08560"
     CDS             complement(3166..5271)
                     /locus_tag="PLK64_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-like protein EF-G2"
                     /protein_id="HPV79838.1"
                     /translation="MSKATAARELSSAPVPASPEQIRNVVLVGPGGAGKSTLFEHLVA
                     ARVPGRRPHEGEHRRSVGVEVAAVESGGLTVNLIDTPGYPDFVGELRAGLRAADAAIF
                     VISGADGIDGATALLWQECASVGMPRIIAVSKLDMPRSDYADTIATCRRVFGDAQPVY
                     LPVYTDDKTVVGSMALISQYVADGTGSRRDPSEDEAAAIEEQRGTLIEAIIEESEDDS
                     LLDRYLSGEDLDVAQLIADTKTAIGKGTFFPVLPISPATGVGMEEIFELIETAFPSPA
                     ARPAPAATTPAGGDVAGLACDPNGPLVAEVIRTSSDPYVGRLSLVRVFSGTLRAEEVV
                     HVSGHLDTFLGHELEGHPAHDDDERVGPLSSPLVDSLRTKEVAIAGDIALVAKLAKAE
                     TSDTLSAKDHPVLVEPWVLPEPLLPVAIHAKSKADEDKLASALQRLVAEDVTMRMEHN
                     AETHQVVLWAMGQAHIEELLGRLAERYGVQVEAEPFRTALRETFVKKVIVKGRHVKQS
                     GGHGQYAVCDLEIEPLPRGGGFEFVDKVVGGAVPRNFIPSVEKGARGQLDKGVLAGYP
                     LVDVRVTLFDGKAHSVDSSDMAFQTAAAMALKEAANESTVALLEPIDAVDITVSDDYV
                     GAVMSDLRGRRGQVLGTEPARESGWTIVHAEVPQTELSRYPIDLRSVSHGTGSFARRQ
                     LRYDYMPADKAKEYAKPEK"
     gene            5452..5580
                     /locus_tag="PLK64_08565"
     CDS             5452..5580
                     /locus_tag="PLK64_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79839.1"
                     /translation="MSALPRRRHGQQTDAQRGPQMLFIDLTRARDNDDADPDDGGE"
     gene            complement(5670..6461)
                     /locus_tag="PLK64_08570"
     CDS             complement(5670..6461)
                     /locus_tag="PLK64_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPV79840.1"
                     /translation="MRIATWNVNSIRSRIDRVEAWLARSDVDVLAIQETKAKADQFPM
                     ERLQACGYEVAHHGLNQWNGVAILSRVGLTEVRQGFPGMPSWGEPPAQEARALGATCG
                     GIQVWSIYAPNGRHLTDPHMDYKMEWLGALRDEAAGWLAADPAAQVVLMGDWNIAPRD
                     EDVWSMEFYADKSHVSPRERAAFDAIVDAGFADVVRPHTPGPGVYTYWDYQQLRFPKK
                     QGMRIDFVLGSPAFAARVDGAVIDREERKGTGASDHAPVVVQLTD"
     gene            6527..7303
                     /locus_tag="PLK64_08575"
     CDS             6527..7303
                     /locus_tag="PLK64_08575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPV79841.1"
                     /translation="MPTALVTGATAGIGLEFARQLAERGSDLVLVARDRQRLEGVAAD
                     LHRRFGVATEVLVADLADRAAVQVVADRLSDASRPVDLLVNNAGFGLKRGFLQNDVEV
                     EENAFDVLCRAVLVLSHAAARAMKTRGQGAIINVSSVASFVAMGSYSAAKAWVTVFSE
                     SLATELAGTGVKVMALCPGFTHTEFHQRAEMNMSKLPDALWLDADQLVTDALADLDRG
                     RVVSVPGTAYKVLVGALNVVPRGLARSVSGGIASRRRPKA"
     gene            7572..7937
                     /locus_tag="PLK64_08580"
     CDS             7572..7937
                     /locus_tag="PLK64_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV79842.1"
                     /translation="MAAEDMDDGVFKALADPTRRALLDALFATDGQTLSSLTARHPQL
                     TRFGVMKHLRILEDAGLVVTQRVGREKRHFLNPVPITAIAARWVGKFSQPYAEALVTL
                     KADLERPSGTEPPPVKESS"
     gene            7934..>8866
                     /locus_tag="PLK64_08585"
     CDS             7934..>8866
                     /locus_tag="PLK64_08585"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPV79843.1"
                     /translation="MTSTQIYQLYIRAEVEAVFQALLDPDFTRRYFHGTAFTDPPRAG
                     QPYALSTPDGTPAVDGTIEVLDPPHRLVQTWHVRYDPAMEAEPPSRVEWTLTRAGEGL
                     TLLRVVHGDLARSPLTAASVRRGWDWVLGGLKTVLETGMPLPPVASDPTDSGVETATP
                     AQDITGDWHRAQAIEINNAVWDQLAGVAAGTATTVGLVRGAYAAAYHWERAKGATPAN
                     EARARYLIGKAWLASARPDQALGYGDRTLAQCEAHGLGDFDLAYAHELRARSLAGLGR
                     DAEALQAWAAALAVEIADPEDREIVEADFADAPTL"
BASE COUNT         1349 a         2972 c         3145 g         1400 t
ORIGIN      
        1 gctacgtggg ctacgaggag ggcggccagc tcaccgaggc cgtgcggcgc aagccctaca
       61 gcgtgatcct gttcgacgag atcgagaagg cgcacccgga cgtgttcaac gtgctcctgc
      121 aggtgctcga cgacggccgg ctcaccgacg ggcaggggcg cacggtcgac ttccgcaacg
      181 tcatcctcgt catgacctcg aacatcggca gccagttcct cgtcgacccg atgttgaccg
      241 acgaggccaa gcgcgaggcc gtgatgggga tggtgcgggc gtcgttcaag cctgagttcc
      301 tcaaccggct cgacgaggtc gtcatcttcg acccgctgtc gacggaggag ttggcgcgga
      361 tcgtcgagtt gcaggtgcgg gcgctgtctg cgcggttggc cgaccggcgg atcacgctgg
      421 aggtcaccga ggccgccaag gaatggctgg cggtcacggg ttacgaccct gcctatgggg
      481 ctcgtccgtt gcgccggttg gtgcagcgcg agatcggcga ccggttggct cgggcgctgt
      541 tggccggcga ggtccgggac gggtcgacgg tcaccgtgga tcgcgacgac gaggccgact
      601 cgctcatcct gcgctgacgt tttctgctcg gtttcctttg ccgggtcgag gtattcggct
      661 atctcctgac gatggcgaaa tacctcgacc cggcgggggt ggggagcggc tctacgatca
      721 cggcatgacg aatccgctca tcgcatcgtt ggcgaaggcc gtcgaggcgg cccccaccga
      781 tgttgcgctg cggctgcacc tggccgagct gctgctggct gacggccaac tcgatgccgc
      841 gctggggcat tgcgcgaccg ctctgcaaca ggacccggcc agtgggccag cgcgggagct
      901 catgggccgg gcgctgggcg gcataccgca accgccgacg ggaccacctg ccgaggcgac
      961 gcccgcggat tcgggacagg cgcacggatt cgattggagt gcagcggagt ccgaggtgaa
     1021 ggacatcgca cccgagccga tgttcgtcga gggcgacgcc ggtgcggagc cggaggtcgg
     1081 tgcctgggac gtcgagcgag cgaccgtgcg actggccgac gtgggcggga tgcgtgaggt
     1141 caaggaacgc atcctcgtgt cgttcctagc gccgatgcag aacccggaga tgcgcaagct
     1201 ttatggcaaa agtctgcgcg gcggcatcct gctctatggg ccgccgggat gtgggaagac
     1261 gttcatcggg cgggccatcg ccggagagat gggtgcgcag ttcatcaacg tgggcttgtc
     1321 cgacgtgctg gacatgtata tcggccagtc cgagcgcaac ctgcacgagc tgttccagat
     1381 cgcccgacgc aacgcccccg tcgtcatctt cctcgacgag gtcgatgcgc tgggccagaa
     1441 gcgcagcctg acccggcact cggcgatgcg cggaacggtc aaccagttgc tcgccgagct
     1501 ggacggagtg tcggacagca acgagggggt ctacgtcgtc ggcgcgacga accagccgtg
     1561 ggacgtcgat ccggcgctgc gccggcccgg gcggttcgat cggaccctcc tggtgctgcc
     1621 tcccgaccag gaggctcggg aggcgatcct cggctaccac ctcaaggatc gacccatcga
     1681 gggcatcaac ctcgcttccc tggccaaggc aaccgacggc tactccgggg ctgacctcgc
     1741 ccatatctgc gagagtgccg ccgagcgggc gatgatggag ggaatccgca gcggcgccgt
     1801 gcggatgatc cggatggacg acctgtatgc cgccctccgc gaggtccgcc cgtcgaccgg
     1861 gccgtggttc gacgcagcgc gcaacgtcgt gcagttcgcc aacaccgacg ggacctacga
     1921 cgagttgcgc acgtatatga agcgcacgaa gaagctctga gggcgaatgg ccagaaacgt
     1981 cacgccggac ccgagcgaac gggtgcggac ctatctcttg cttggccgcg acgcggaggc
     2041 gctcgcagcg tgcagccggg ccctcaccca caacccgacc gacaccgggt tgctcacctt
     2101 gtcgtcgatc gcctcttacc gtctcgggca gcacgacgac gctattcggt tcgccatgat
     2161 ggccatctcc tcaggccccg actccgactc cgcgcatcga gcgttggcga cggccctgct
     2221 cactgtggga agggcgcagg aggcgacgtc ggcggcatac accgctgctg cgttagcgcc
     2281 gcacaactgg gtcaaccatc tgcgctactc acagtgcctc atggccatgc ccgacagccg
     2341 cgatgctgcc tggcaagcgg caaaccgggc cgtggagctg gctccgcagg agtcgctggc
     2401 gcactaccag gtggcctgcg tggtgtatcc cgtcgggccg agcacccggg cccagctcga
     2461 cctcgccgaa cgcgcgatgc gagaggcgct gcgcctggag ccttcgaacg cgacctacca
     2521 gcacgaactg gcgcggctga agctcgttcg ctccggggtg gacggggtgg gcggcggggc
     2581 ggcgtcggag gtgctggccg gcttcacggg agccgtgctc gctgacccga ttggggtggg
     2641 caagggggcg ttggagaaca tccgcctcgt gttgcgtcgg ttagtccttg tgcagagcca
     2701 gatggtcatg gcagcggcat gtgtcgggat cctcatgctc ggcggcctcc cgtccaggcc
     2761 ggggaaagtc gtgctcgaca gttcctcggc cctgcggggg aaactcgggc ttctggccgt
     2821 cgtcgtggcc ttgtgtatct actttgtcgt ggcctggcgt cggaatctcg tcggtccgag
     2881 ccagcagaac gtcatccggc aggtcgttcg tggtgatcga cggttatcgg tcggcgctgg
     2941 cctcctcgcc gcggcgctgc cggtcatggc tgcgggtgcg ttcctgccgg gcgacgggcc
     3001 ggcgatggcc gcggcaggga cattcctgtt ggcgagtgcg gcggccatcc tggcccgggc
     3061 gaaatagccg aaccccgcgc cctgcccctg ccgggtcgag gtattcggcc atcatgaggc
     3121 gatggcgaaa tacctcgacc cggcggaggg gtcggcaggg gtggcctact tctcgggctt
     3181 ggcgtactcc ttggccttgt cggccggcat gtagtcgtag cgcaactgcc ggcgggcgaa
     3241 ggaccccgtg ccgtgcgaga ccgaacgcag gtcgatcggg tagcgcgaca actcagtctg
     3301 gggcacctcg gcgtggacga tcgtccagcc cgactcgcga gccggctcgg tgcccaacac
     3361 ctggccgcgg cgaccgcgca ggtcggacat gaccgcgccc acgtagtcgt ccgacaccgt
     3421 gatgtcgacc gcgtcgatcg gctcaagcag agccaccgtc gactcgttgg cggcctcctt
     3481 gagggccatc gccgccgccg tctggaaggc catgtcggat gagtcgaccg agtgcgcctt
     3541 gccgtcgaag agcgtcacac gcacgtccac caacggataa cccgcgagaa cgcccttgtc
     3601 gagttgacca cgggcgccct tctccaccga ggggatgaag ttgcgcggca cggcgccacc
     3661 gacgaccttg tcgacgaact cgaagccacc accgcggggg agtggctcga tctccaggtc
     3721 gcacacggca tactgcccat gcccgccgga ctgcttgacg tgccggccct tgacgatgac
     3781 cttcttgacg aacgtctcgc gcaacgcggt ccggaacggc tcggcctcga cctgcacgcc
     3841 gtagcgctcc gccagccgcc cgagcaactc ctcgatgtgg gcctggccca tcgcccagag
     3901 gacgacctgg tgggtctcgg cgttgtgctc catccgcatc gtcacgtcct cggcgacgag
     3961 gcgctgcaag gccgacgcga gcttgtcctc gtcggccttg gacttggcgt ggatcgcgac
     4021 gggcagcagc ggctcgggca gcacccacgg ctcgacgagc accgggtggt ccttggccga
     4081 caaggtgtcg gaggtctcgg ccttggcgag cttggcaacc agcgcgatgt caccggcgat
     4141 ggccacctcc ttggtgcgca acgaatccac cagcggcgac gacaacgggc cgacgcgctc
     4201 gtcgtcgtcg tgggccgggt ggccttcgag ctcgtggccg aggaaggtgt cgaggtgacc
     4261 cgagacgtgg acgacctcct cggcgcgcag ggtgccggag aacacccgga ccagggagag
     4321 ccgcccgaca taggggtcac tgctcgtgcg aatgacttcg gcgacgagcg gaccgttcgg
     4381 gtcgcacgcc agcccggcga catcgccgcc ggcaggcgtg gtggctgctg gcgccgggcg
     4441 cgcagccggc gacgggaacg ccgtctcgat gagctcgaag atctcttcca tgccgacacc
     4501 ggtcgccggc gagatgggca ggacggggaa gaacgtgccc ttgccgatcg ccgtcttggt
     4561 atcggcgatg agctgggcga cgtcgaggtc ctcaccggac aggtagcggt ccaacagcga
     4621 gtcgtcctcg gactcctcga tgatcgcctc gatgagcgtg ccgcgctgct cctcaatggc
     4681 ggcggcttcg tcctcggagg gatcgcggcg gctgcccgtg ccgtcggcga cgtactggct
     4741 gatgagggcc atcgacccga cgaccgtctt gtcgtcggtg tagaccggca ggtagaccgg
     4801 ctgggcgtcg ccgaagaccc gacggcaggt ggcgatggtg tcggcatagt ccgatcgcgg
     4861 catgtccagc ttgctcacgg cgatgatccg cggcataccg acggatgcgc actcctgcca
     4921 cagcagggcg gtcgcgccgt cgatgccgtc cgcgccggag atgacgaaga tggccgcgtc
     4981 ggcggcgcgc aaccccgcgc gcagctcgcc gacgaagtcg gggtatccgg gggtgtcgat
     5041 gaggttgacc gtcagcccgc cgctctcgac ggcggcaacc tcgacgccca ctgagcgtcg
     5101 gtgctcgccc tcgtggggcc gtcgacccgg cacgcgcgcc gcgaccaggt gctcgaagag
     5161 ggtggatttg cccgcaccgc cggggccgac gagcacgaca ttgcggatct gctcggggct
     5221 cgcggggacc ggtgcggacg acagttctct ggcggcggtt gctttgctca cgggctccac
     5281 ctttctctgt gccgacccac cccgcaaggg gaccgagggc ccgcctcagc gcgcctggcc
     5341 ggatgcttcc cgacagcgta tgccgcccgc ccggcatccg gcggagcggg gcccgaacgg
     5401 gggcggtgag cttgctggaa ccctggcgtg agaccgaccc cgactgtcac catgagcgca
     5461 ttgccccgcc gacggcacgg gcagcagacc gacgctcaga gaggaccgca gatgctgttc
     5521 atcgacctca cccgcgcccg tgacaatgac gacgccgacc ctgacgacgg cggcgagtga
     5581 ccacaccggt ctgacatcgc gcccaccgct gtggtgggcg acacgcggac ggtgagcggg
     5641 cggcataccc ctgaggtatg ccgcccgctt cagtcggtga gttggacgac gaccggcgcg
     5701 tggtctgagg ccccggtgcc cttgcgctcc tcgcggtcga tgaccgcgcc gtccacgcga
     5761 gctgcgaacg ccggcgagcc gaggacgaag tcgatgcgca tgccctgctt cttggggaag
     5821 cgcagttgct ggtagtccca gtaggtgtag acgccgggcc cgggtgtgtg cggccgcacg
     5881 acgtcggcga agcccgcgtc gacgatggcg tcgaaggccg cccgctcacg cggcgacacg
     5941 tgcgacttgt cggcgtagaa ctccatgctc cacacgtctt cgtcgcgcgg cgcgatgttc
     6001 cagtcgccca tgaggacgac ctgggcggcg gggtcggccg caagccaacc ggcggcctcg
     6061 tcgcgcagcg cgccgagcca ttccatcttg tagtccatgt gcgggtcggt caggtgccgt
     6121 ccgttggggg catagatcga ccacacctgt atgccgccgc aggtcgcccc gagcgcgcgg
     6181 gcctcctggg cgggcggctc gccccaagac ggcataccgg ggaagccttg gcggacctcg
     6241 gtgagaccga cccgggagag gatggcgaca ccgttccatt ggttgaggcc gtggtgggcg
     6301 acctcgtagc cacacgcctg cagccgctcc atcgggaact ggtcggcctt ggccttggtc
     6361 tcctgaatcg ccaagacatc gacgtcggag cgagccagcc aggcctcgac gcggtcgatg
     6421 cggctgcgga tggagttgac gttccaggtg gcgatgcgca cggccccagc gtaggtgcag
     6481 ccggtgacag ggggtgggcc gcgggcgcgc gccctacgct ggcggtatgc cgaccgctct
     6541 tgtcacaggt gccaccgccg ggatcgggct ggagtttgcc cgccaactcg ccgagcgagg
     6601 ctccgacctc gtgttggtcg cgcgggatcg gcaacggttg gagggggtcg cggctgacct
     6661 gcaccggcgc ttcggtgtgg cgactgaggt gctggtggcc gatctcgccg atcgggccgc
     6721 ggtccaggtc gtcgccgacc ggctctccga cgcttcccgg ccggtcgatc tgctggtgaa
     6781 caacgctggc ttcggtctca agcggggctt cctgcagaac gacgtcgagg tcgaggagaa
     6841 cgccttcgac gtgctctgcc gagcagtcct cgtcctgtcg catgcggcgg ctcgggcgat
     6901 gaagacccgg ggtcagggag cgatcatcaa tgtgtcgtcg gtagcgagtt ttgttgcgat
     6961 gggctcgtat tcggcggcca aggcctgggt gacggtgttc tcggagagcc tggccaccga
     7021 gttggccggg acgggcgtca aggtgatggc gctgtgcccg gggttcacgc acaccgagtt
     7081 ccatcagcgg gccgagatga acatgtcgaa gctgcccgac gcgctgtggt tggacgccga
     7141 ccagctcgtg accgatgcgt tggccgacct cgaccggggc cgcgtcgtgt cggtgccggg
     7201 cacggcatac aaggtgctcg tcggggcgct caacgtggtg ccgcgcggcc tggcgcgctc
     7261 ggtgagcggc gggatcgcct cccgtcgccg gcccaaggcc tgatcccgag ctaaccccgc
     7321 ccccctgcca cggagatagc gctcgcatgg tcatttgtgg gggtcgcctc gccctcctga
     7381 cccggtgata gcgctcgcat ggtcatgtgt ggggtcgccg ggccctcctg acccggtgat
     7441 agcgctcgca tggtcacctt tgggggtctg gacggccaaa atgcccggaa agatgaccat
     7501 gcgagcgcta tctccggggg agcaggggga gggcgttgac aggtgactaa agggtcacct
     7561 attgtctcgg catggcggcc gaggacatgg acgacggggt cttcaaagcc ctcgccgacc
     7621 cgactcgacg ggcgctgctg gatgccctct tcgccactga cgggcagacg ctgagcagcc
     7681 tcaccgccag gcacccccag ctcacccggt tcggggtgat gaagcacctg cggatcctcg
     7741 aggacgccgg gctcgtcgtc acccaacgcg tcgggcggga gaagcgccac ttcctcaacc
     7801 cggtgccgat caccgccatc gccgcccgct gggtcggcaa gttcagccag ccgtatgccg
     7861 aggcactcgt cacgctcaag gccgaccttg agcgcccatc gggcaccgaa ccgccccccg
     7921 tgaaggagtc gtcatgacga gcacccagat ctaccagctc tacatccgcg ccgaggtcga
     7981 ggcggtcttc caggccctgc tcgacccgga cttcacccgg cgctacttcc acggcacggc
     8041 cttcaccgac ccgccgcggg ccggtcagcc ctacgccctg tcgacgcccg acggcacgcc
     8101 cgccgtcgac gggaccatcg aggtgctcga cccgccgcac cggctcgttc agacctggca
     8161 cgtccgctac gacccggcaa tggaggccga gccgccgagc cgcgtcgagt ggacccttac
     8221 ccgcgcaggc gaggggttga ccctgctgcg cgtcgttcat ggcgacctcg cccgcagccc
     8281 gctcacggcg gccagtgtca ggcgcggttg ggactgggtg ctcggcgggc tcaaaaccgt
     8341 tctcgaaacg ggtatgccgc tgcctcccgt cgcttccgac cccacggaca gcggggtcga
     8401 gaccgcgacc ccggcgcagg acatcaccgg agactggcat cgggcccagg cgatcgagat
     8461 caacaacgcc gtctgggacc aactcgctgg agttgcggcg ggcaccgcca ccacggtggg
     8521 cctggtccgc ggggcgtatg ccgcggcata ccactgggag cgggccaagg gagcgacgcc
     8581 ggcgaacgag gcacgcgctc gctacctcat cggcaaggcc tggctggcgt ccgcgcgacc
     8641 cgatcaggcg ctcggctatg gcgaccggac gctggcccaa tgcgaagccc acgggttggg
     8701 cgacttcgac ctggcctatg cccacgagtt gcgcgctcgg tcgctggccg gactgggccg
     8761 ggacgctgag gcgctccagg cgtgggcggc ggcgcttgcg gtcgagatcg ccgatcccga
     8821 ggaccgcgag atcgtcgagg ccgacttcgc cgatgcgccc accctg
//
LOCUS       DAOMZY010000128         8851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_7460_length_8851_cov_6.167576, whole genome shotgun sequence.
ACCESSION   DAOMZY010000128 DAOMZY010000000
VERSION     DAOMZY010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8851
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7460_length_8851_cov_6.167576"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="PLK64_08590"
     CDS             <1..688
                     /locus_tag="PLK64_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HPV79844.1"
                     /translation="LPVVRVSALKALEGDPEWAKSVQALMDAVDEYIPEPERATDKPF
                     LMPIEDVFTITGRGTVVTGRVERGVLKVNEEVEIVGIHEKSTKSTVTGVEMFRKLLDE
                     ARAGENVGLLLRGVKREDVERGQVVIKPGTMTPHTEFDAQVYILSKDEGGRHTPFYDN
                     YRPQFYFRTTDVTGVVKLPEGTDMVMPGDNTEMAVTLIQPIAMDDGQPFSIREGGRTV
                     GSGKVIKVTK"
     gene            946..>2120
                     /locus_tag="PLK64_08595"
     CDS             946..>2120
                     /locus_tag="PLK64_08595"
                     /inference="COORDINATES: protein motif:HMM:NF002759.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HPV79845.1"
                     /translation="MSLHTAFDPDSTAQGLIDFVDASPTPFHAVDSAIRLLAAEGFTA
                     LDESAPFPSQPGRYYIARGGALVAWIETGGMPPTAPFRVIGTHTDSPNLRVKPRPDLV
                     KAGWQMVAVEPYGGLLINSWLDRDLGLAGRVAVRDNSAPTGVSTRLFHDRAPLLRVAQ
                     LAIHLDRGVNERGLVLNPQQHLVPLWGVGEAPASVAAYLGEQVGADPGDVLAWDAMLH
                     DTQPSARIGRAQELIAAPRLDNLASSYAAVRALLAVAASGGTSADQGADAAYRPVIAL
                     FDHEEVGSRSERGADSPMLRGILERIVLAAGGSRDDLWRALAGTIVASADMAHATHPN
                     YSELHEPGHRIEMNGGPVLKVQTELRYATDAVGAAALHLACEQADVPLQVFVTRSDLP
                     "
     assembly_gap    2121..2220
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2221..2441
                     /locus_tag="PLK64_08600"
     CDS             <2221..2441
                     /locus_tag="PLK64_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944837.1"
                     /note="catalyzes the removal of amino acids from the N
                     termini of peptides; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HPV79846.1"
                     /translation="CEQADVPLQVFVTRSDLPCGSTIGPITAGQVGVTTVDLGAPMLS
                     MHSIRELAGVADQAMYAAALAAFLAPVR"
     gene            complement(2532..3455)
                     /locus_tag="PLK64_08605"
     CDS             complement(2532..3455)
                     /locus_tag="PLK64_08605"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPV79847.1"
                     /translation="MIDLAAVRTLDAVREHGSVVAAAAALGYTPSAVSQQIKRLERHT
                     GVQLLERVGRGVALTELGRRLADDGNRVLGDLEAIEAQLAVAHGSAPLHGQVRIAAFS
                     TGVRGLVAPALATLRHTDPGLDVRVVESDPHEALDLVAGGQVDTALVHNWGDLPLPIP
                     AHVEDVHLGIDTADVLVPRDHPLATRSVITAADLDGLVWTCAPVGSVCHGWLVHLHDA
                     HGLRPDVRYWAMEFSSQIGLVEHGNAVALVPRLGREALPSGVIAVPVDDPVTRRILQV
                     WRRTTAPSPAIRRVHDVLTSAMDAVAVAHPR"
     gene            3528..4409
                     /locus_tag="PLK64_08610"
     CDS             3528..4409
                     /locus_tag="PLK64_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPV79848.1"
                     /translation="MTPRDRLLALAVVTVWGLNFVVIDWGLPGVPPLVFVTMRFVLVL
                     LPAIFFVPRPAGRWQDIALVGLLLSVGQFGLLYSAMSAGMPPGLASLVLQIQAAFTVV
                     FAATLLRERPSRRQVIGVGLGLLGLGIVALGRGGSVPLGALGLCVAAAASWAGGNIAV
                     RRLPGVGGLSLTVWSGLVVPIPLFALALLVEGPETVMHALTHLSVPSLLSTAYTAVLA
                     SLFGYGVWNSLMGRHRAAEVAPYSLLVPVVGMTAALVFRGERPGLLALVGGALLVAGV
                     AVTTLRRRQVAGELVSR"
     gene            4757..5065
                     /gene="rpsJ"
                     /locus_tag="PLK64_08615"
     CDS             4757..5065
                     /gene="rpsJ"
                     /locus_tag="PLK64_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPV79849.1"
                     /translation="MAGQKIRIRLKSYDHEVIDSSARKIVDTVTRAGATVVGPVPLPT
                     EKNVFVVIRSPHKYKDSREHFEMRTHKRLIDIIDPTPKAVDSLMRLDLPADVNIEIKL
                     "
     gene            5076..5729
                     /gene="rplC"
                     /locus_tag="PLK64_08620"
     CDS             5076..5729
                     /gene="rplC"
                     /locus_tag="PLK64_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPV79850.1"
                     /translation="MSNATTRTVKGVLGEKLGMTQVWDADNRLVPVTVIKVGPNVVTQ
                     VRGDADGYPAVQIGYGEIDPRKVNKPMKGHFERAGVTPRRHLVELRTADASDYALGQE
                     ITVSTFEAGQLVDATATTKGKGFAGVMKRHGFHGVSSSHGAHRNHRKPGSIGACATPG
                     RVFKGVRMAGRMGGERQTTQNLAIQAVDADKGLLLIKGAVPGPRGAIVLVRSASKGA"
     gene            5735..6766
                     /gene="rplD"
                     /locus_tag="PLK64_08625"
     CDS             5735..6766
                     /gene="rplD"
                     /locus_tag="PLK64_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPV79851.1"
                     /translation="MATTTALPADIFDVQVNVPLIHQVVAAQLAAARQGTHSTKTRGL
                     VSGGGKKPYRQKGTGRARQGSTRAPQFAGGGVVHGPQPRDYSQRTPKKMKAAALRGAL
                     SDRARNGRIHVIDVLVSGDAPSTSTAAAVLAGLTDRTKFLVVLERGDQVGLKSVRNLD
                     SVHVLAVDQLNTYDVLCADDVVFTAGALATLTASSKAAATAPVAATVEKADKAEKVEK
                     AEKVEKADKAEKPAKAEKAASLVDAAEAAPAPKATKAAKVAEAPAEAAAELPAAAAAP
                     LADGSAPEGYSVKGNADSGLYHEPDGRWYDATVAEFWFKSAADAEAAGFKKAGSRAKS
                     TAKDGEDAK"
     gene            6763..7062
                     /gene="rplW"
                     /locus_tag="PLK64_08630"
     CDS             6763..7062
                     /gene="rplW"
                     /locus_tag="PLK64_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPV79852.1"
                     /translation="MSIHEKNPRDILIAPVVSEKSYGLLDEGKYTFVVDPRANKTEIK
                     IAVEAVFGVKVDKVHTLNRQGKTRRTRFGAGKRKDTKRAIVSLREGSIDIFGSVS"
     gene            7091..7927
                     /gene="rplB"
                     /locus_tag="PLK64_08635"
     CDS             7091..7927
                     /gene="rplB"
                     /locus_tag="PLK64_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPV79853.1"
                     /translation="MGIRKFKPTTPGRRGSSVADFVEVTRSTPEKSLVRPIAKTGGRN
                     NAGRITTRHIGGGHKRAYRLIDFRRHDKDGVPATVAHIEYDPNRTARIALLHFADGEK
                     RYILAPNKLKQGDVIESGPSADIKPGNALPLRNIPTGTVIHAIEIKPGGGAKVARSAG
                     AKVQLVAKDGAFAQLRMPSGEIRNVDARCRATIGEVGNAEQSNINWGKAGRMRWKGKR
                     PTVRGVAMNPIDHPHGGGEGKTSGGRHPVSPWGQQEGRTRKAHKESDKLIVRRRRSGK
                     KR"
     gene            7940..8221
                     /gene="rpsS"
                     /locus_tag="PLK64_08640"
     CDS             7940..8221
                     /gene="rpsS"
                     /locus_tag="PLK64_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPV79854.1"
                     /translation="MPRSLKKGPFIDDHLQKKVDVQNEAGTKNVIKTWSRRSVVSPAM
                     LGHTFAVHDGRKHIPVFVTESMVGHKLGEFAPTRTFKGHVKDDKKGRRR"
     gene            8251..8625
                     /gene="rplV"
                     /locus_tag="PLK64_08645"
     CDS             8251..8625
                     /gene="rplV"
                     /locus_tag="PLK64_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HPV79855.1"
                     /translation="MEAKAVARHVRVTPMKARRVIDLIRGKNATDAVATLMFAQQAAS
                     EPVRKVLQSAMANARVKADAASEAFDERLLVVSAAYVDEGATMKRFRPRAQGRASRIN
                     KRTSHITVVVTQPEIATRKGTR"
     gene            8625..>8851
                     /locus_tag="PLK64_08650"
     CDS             8625..>8851
                     /locus_tag="PLK64_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPV79856.1"
                     /translation="MGQKVNPHGFRLGITTEHKSRWFADSTKTGQRYRDYVKEDVEIR
                     RLMEKGMERAGISRVEIERTRDRVRVDIHTAR"
BASE COUNT         1429 a         3058 c         2977 g         1287 t
ORIGIN      
        1 ccttccggtc gtgcgggtct cggcgctcaa ggctctcgag ggcgaccccg agtgggccaa
       61 gtcggtccag gccctcatgg acgccgtgga cgagtacatc ccggagcccg agcgcgccac
      121 cgacaagccg ttcctcatgc ccatcgagga tgtcttcacc atcaccggcc gtggcaccgt
      181 tgtcaccggt cgtgtcgagc gtggtgtcct caaggtcaac gaggaggtcg agatcgtcgg
      241 catccacgag aagtcgacca agtcgaccgt cacgggcgtc gagatgttcc gcaagctgct
      301 cgacgaggcc cgcgccggtg agaacgtcgg cctgctgctt cgtggcgtca agcgtgagga
      361 cgtcgagcgc ggccaggtcg tcatcaagcc cggcacgatg accccccaca ccgagttcga
      421 cgcacaggtc tacatcctgt cgaaggacga aggtggccgt cacacgccgt tctacgacaa
      481 ctaccgcccc cagttctact tccgcaccac ggacgtgact ggcgtcgtga agctgcccga
      541 gggcaccgac atggtcatgc ccggtgacaa caccgagatg gccgtgaccc tgatccagcc
      601 catcgccatg gacgacggcc agccgttctc catccgcgag ggtggccgca ccgtcggctc
      661 gggcaaggtc atcaaggtca ccaagtgatc tgacgtcgcc tcgcgacagc gcacttcgtt
      721 cgatgggccc cagccactcg gctggggccc atcggcgtca catcgtgtgg ggtcccgggc
      781 cgcgtccccg gcctcggctc agccctccga gtttggccgc catggcgctc acatcggccg
      841 atggcggtga gatcaggccg cccttcacga gtttgaccgc catggcggcc aaacgccgga
      901 cagacccacc gagctgctga ccggcagcgc gggtaaggtc gatgggtgag cctgcacacc
      961 gccttcgacc ccgactccac ggcccagggg ttgatcgact tcgtcgatgc ctccccgacg
     1021 cccttccacg cggtcgactc cgcgatccgg ctgctcgcgg ctgaaggctt caccgcgctg
     1081 gacgagtcgg cgccgttccc cagccagcca ggccgctact acatcgcccg cggcggcgcc
     1141 ctcgtggcct ggatcgagac agggggtatg ccgcccaccg cgcctttccg ggtcattggc
     1201 acccacaccg actcgcccaa tctgcgggtc aaaccacgcc ccgacctggt caaggccggc
     1261 tggcagatgg tggctgtcga gccctatggc gggctgctga tcaactcgtg gctcgaccgc
     1321 gacctcgggc tcgccggacg ggtcgccgtg cgcgacaact cagccccaac tggggtgtcg
     1381 acccggctgt tccacgaccg ggcccccctc ctgcgggtcg cccagctcgc gatccatctg
     1441 gaccgcggcg tcaacgagcg gggtctggtc ctcaacccgc aacaacacct ggtgcccctg
     1501 tggggggtgg gggaggcgcc agcgagcgtc gcggcatacc tcggggagca ggtgggcgcc
     1561 gacccgggtg acgtgctcgc ctgggacgcg atgctccacg acacccagcc gtcggcccgc
     1621 atcggccgcg cccaggagct catcgccgcg ccgcgcttgg acaacctcgc gagctcgtat
     1681 gccgccgtcc gcgccctgtt ggcggtcgct gcctccggtg gcacgtcggc cgaccagggg
     1741 gccgacgcgg cataccggcc ggtcatcgcg ctgtttgacc acgaggaggt gggcagccgc
     1801 agcgagcgcg gcgccgactc accgatgctg cgcgggatcc tcgaacgcat cgtcctggcc
     1861 gccggtggga gtcgcgacga cctctggcgg gcgctcgctg ggacgatcgt cgcgtcggcc
     1921 gacatggccc acgccacgca ccccaactac tcggagctac acgagcccgg gcaccggatc
     1981 gagatgaacg gcggcccggt gctcaaggtg cagaccgagt tgcgctacgc caccgacgcg
     2041 gtgggcgcag cggcgttgca cctggcgtgc gaacaggcgg atgtgccgtt gcaggtgttc
     2101 gtcacccgca gcgacctgcc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 cgtgcgaaca ggcggatgtg ccgttgcagg tgttcgtcac ccgcagcgac ctgccctgcg
     2281 gctcgaccat cggcccgatc acggccggcc aggtgggggt gacgacggtc gacctcggcg
     2341 cgccgatgtt gtcgatgcac agcatccgcg agctcgccgg cgtcgccgac caagcgatgt
     2401 atgccgcggc gctcgctgcc ttcctcgccc cggtgcgctg accgaagccg cctcgatcgc
     2461 gggcgtcggc gccgccttga tcgcgggcgc cggtgccggc gccggtgccg ctgccgctgt
     2521 cggcgccccc gtcagcgcgg gtgggcgacg gccacggcgt ccatcgccga ggtgaggacg
     2581 tcgtggaccc ggcggatggc cgggctcggc gccgtcgtcc ggcgccagac ctgcaggatg
     2641 cgccgggtca ccgggtcgtc gaccggcacg gcgatgacgc cggacggcag cgcctcccgg
     2701 cccaaccgcg gcaccagggc gacggcgttc ccgtgttcga ccaggccgat ctgtgaggag
     2761 aattccatcg cccagtagcg cacgtcgggc cgcaggccat gggcgtcgtg caagtggacg
     2821 agccagccgt gacacacgct gccgaccggg gcgcaggtcc acaccaaccc gtccaggtcc
     2881 gcggcagtga tgaccgatcg agtggccagg ggatggtcgc gggggaccag cacatccgca
     2941 gtgtcgatcc ccaggtggac gtcctcgaca tgcgcgggga tcggcagcgg caggtcgccc
     3001 cagttgtgca cgagcgcggt gtcgacctgg cccccggcga cgaggtcgag cgcctcgtgc
     3061 gggtcgctct cgaccacccg gacgtcaagc ccgggatcgg tgtggcgcaa cgtggccagg
     3121 gcgggggcga ccagcccgcg cacccccgtg gagaaggccg cgatccgcac ctgaccgtgc
     3181 aggggagcac tcccgtgggc caccgcgagc tgcgcctcga tcgcctcgag atcgccgagg
     3241 acccggttgc cgtcgtcggc aaggcggcgg cccaactcgg tgagcgccac cccgcggccg
     3301 acccgttcga gcaactgcac tccggtatgc cgttcgagcc gcttgatctg ctgggacacc
     3361 gccgaggggg tgtagccgag cgctgcggcg gccgcgacga cggagccgtg ctcgcggacg
     3421 gcatcgagcg tccgcactgc ggcgagatcg atcatgtagc gacactaccg atacaggtgc
     3481 aggaatatgc gcttgtcctg aaacgtcaga cgcgacaggg tggacccgtg accccccgtg
     3541 atcgtctgct cgccctcgcc gtcgtcacgg tctgggggct caacttcgtc gtcatcgact
     3601 ggggtctgcc cggagtgccg ccgttggtct tcgtgacgat gcgcttcgtc ctcgtgctgc
     3661 tgccggcgat cttcttcgtt ccgcgccccg cagggcggtg gcaggacatc gccctcgtcg
     3721 ggctgctgct cagcgtcgga cagttcgggt tgctctacag cgcgatgtcc gcgggtatgc
     3781 cgcctgggct cgcctcgctg gtcctgcaga tccaagccgc cttcaccgtc gtgttcgccg
     3841 cgacgctgtt gcgggagcgg cccagccgtc gccaggtcat cggggtcggg ctcgggctgc
     3901 tggggttggg gatcgttgcg ttgggtcgcg ggggatcggt cccgttggga gcgctcgggt
     3961 tgtgtgtggc ggccgccgcg tcctgggcgg gcggcaacat cgccgtgcgc cgtctgccgg
     4021 gcgtgggcgg cctatcgctg acggtgtggt cggggctggt cgtcccgatc ccgctcttcg
     4081 ccctcgcgtt gctcgtcgaa ggacccgaga cggtcatgca cgcattgacc cacctgtcgg
     4141 tgccgagcct gctgtccacg gcatacaccg cggtgttggc ctcgctgttc ggctacggcg
     4201 tgtggaactc cctcatgggt cggcaccggg ccgccgaggt cgcgccctac tcgctcctgg
     4261 tgcccgtggt cggcatgacg gccgccctgg tgttccgcgg cgagcggccc ggtctgctgg
     4321 ccctcgtggg cggggcgctg ctcgtcgcgg gggtcgctgt gacgaccctg cggcgccgac
     4381 aggtcgcggg cgaactcgtc agtcgatgag gcgagtgacc gtggatcctc ggcgatttgg
     4441 ctgctcacca cgccatctgg cacactagac cggttgcctg acgcgcggcg tcgtgccctg
     4501 ggtggaacca gagccgatgc cgccgcctct cggccggacg cacgatctac gggtctgcgg
     4561 cggcacggac accactggtg gcccgaggga agcgcaggcg ccggctacgg ccggactgaa
     4621 gacacccgat cgttcggggc acttaggtgc ccaggctcgg gccgcggagc gggcgcgaca
     4681 cgcccgaccg cgggggtcgg aggttcgacc tggccgacca agggcaggtg ttggcgagaa
     4741 gagagacggt acgacgatgg cgggacagaa gatccgcatc cgcttgaagt cctacgacca
     4801 cgaggtcatc gacagttcgg cacgcaagat cgtcgacacg gtgacccgcg cgggtgcgac
     4861 ggtcgtcggt ccggtgccgc tgccgacgga gaagaacgtc ttcgtcgtga ttcggtcgcc
     4921 ccacaaatac aaggactccc gcgagcactt cgagatgcgc acgcacaagc gcctcatcga
     4981 catcatcgac ccgacgccca aggccgtcga ctcgctgatg cgtctcgatc tgccggcgga
     5041 tgtcaatatc gagatcaagc tctgaggaca tcacgatgag caacgcgacg acacgcacag
     5101 tgaagggtgt gctgggcgag aagctcggca tgacccaggt gtgggacgcc gataaccggc
     5161 tggttcccgt gaccgtcatc aaggtcggtc ccaatgtggt cacccaggtc cgcggcgacg
     5221 cggacgggta ccccgccgtt cagatcggct acggcgagat cgacccgcgc aaggtcaaca
     5281 agccgatgaa gggccacttc gagcgcgccg gggtcacccc gcgccggcac ctggtcgagc
     5341 tgcgcaccgc cgacgccagc gactacgccc ttgggcagga gatcacggtc tccaccttcg
     5401 aggccggtca gctggtcgac gccaccgcaa cgaccaaggg caagggcttc gccggtgtga
     5461 tgaagcgaca cggattccac ggtgtctcct cctcgcacgg tgcccaccgc aaccaccgca
     5521 agcccggttc gatcggcgcc tgcgcgaccc ccggtcgcgt cttcaagggc gtccggatgg
     5581 caggccggat gggcggcgag cgccagacca cccagaacct cgcgatccag gccgtcgacg
     5641 ccgacaaggg cctgctgctc atcaagggtg ccgttcccgg cccccggggt gcgatcgtcc
     5701 tcgttcgctc cgcgtcgaag ggagcctgac agccatggcg accaccactg cacttccggc
     5761 cgacatcttc gatgtccagg tcaacgtccc gctgatccac caggtcgtcg cggctcagct
     5821 cgcggccgcc cggcagggca cgcactccac caagacccgg ggcctggtct ccggcggtgg
     5881 caagaagccc taccgccaga agggcaccgg ccgcgcccgt cagggttcga cccgtgcgcc
     5941 ccagttcgcc ggcggtggcg tcgttcacgg cccgcagccg cgtgactact cgcagcggac
     6001 ccccaagaag atgaaggccg ccgccctgcg cggggccctc tcggaccggg cccgcaacgg
     6061 ccggatccac gtcatcgacg tgctggtctc cggtgacgcg ccctcgactt cgaccgcggc
     6121 ggcggtgctg gccggtctga ccgaccgcac caagttcctc gtcgtgcttg agcgtggcga
     6181 ccaggtcggt ctcaagagcg tgcgcaacct cgactcggtt cacgtcctgg ctgtcgacca
     6241 gctcaacacc tacgacgtgc tgtgcgccga cgacgtggtc ttcacggccg gtgcgctcgc
     6301 gacgctgact gcctcgtcga aggctgcggc gactgcccct gtggcagcaa cggtcgagaa
     6361 ggccgacaag gctgagaagg tcgaaaaggc tgagaaggtc gagaaggctg acaaggctga
     6421 gaagccggcc aaggcggaga aggccgcgtc gctggtcgac gcagccgagg ccgccccggc
     6481 gccgaaggcc accaaggcgg ccaaggtcgc cgaggctccg gctgaggccg ctgccgaatt
     6541 gccggccgct gctgcggcgc cgctggcgga cggctcggct cccgagggtt actcggtcaa
     6601 gggcaacgcc gactcgggtc tttaccacga gccggatggc cgttggtatg acgcgactgt
     6661 cgcggagttc tggttcaaga gcgctgccga cgccgaggcc gcaggcttca agaaggccgg
     6721 cagccgcgcc aagagcacgg ccaaggatgg ggaggacgcc aagtgagcat ccacgagaag
     6781 aacccgcgcg acatcctgat cgccccggtc gtctccgaga agtcctacgg cctgctcgac
     6841 gaggggaagt acaccttcgt cgtcgacccc cgcgccaaca agaccgagat caagatcgcc
     6901 gtcgaggcgg tgttcggcgt caaggtcgac aaggtccaca ccctcaaccg gcagggcaag
     6961 acgcgtcgta cccgtttcgg ggccggcaag cgcaaggaca ccaagcgagc gatcgtctcc
     7021 ctccgcgagg gctcgatcga catcttcggc agcgtctcct gacgccgcgg tagggatctg
     7081 aggaacacac atgggtatcc gcaagttcaa gcccacgacc cccggtcgcc ggggttcgtc
     7141 ggttgccgac ttcgtcgaag tgacgcggtc gacgccggag aagtcgctcg ttcgcccgat
     7201 cgccaagacc ggtggccgta acaacgccgg ccggatcacc acccggcaca tcggtggcgg
     7261 tcacaagcgc gcctaccggc tgatcgactt ccgtcgtcac gacaaggacg gcgtgccggc
     7321 gactgtcgcg cacatcgagt acgaccccaa ccgcacggca cggatcgcgc tgctgcactt
     7381 cgccgatggc gagaagcgct acatcctcgc gccgaacaag ctcaagcagg gcgacgtcat
     7441 cgagagcggc ccctcggccg acatcaagcc gggcaacgcg cttccgctgc gcaacatccc
     7501 gaccggtacc gtcatccacg ccatcgagat caagcccggt ggcggggcga aggtggcccg
     7561 ctcggccggc gcgaaggtcc agctcgtcgc caaggacggc gctttcgccc agctgcgtat
     7621 gccgtctggc gagatccgca acgtcgacgc ccgctgccgc gccaccatcg gtgaggtcgg
     7681 caacgccgag cagagcaaca tcaactgggg caaggccggc cggatgcggt ggaagggcaa
     7741 gcgcccgacc gtccgtggtg tggccatgaa cccgatcgac cacccgcacg gcggtggcga
     7801 aggcaagact tccggtgggc gtcacccggt gagcccgtgg ggccagcaag aaggccgcac
     7861 gcgcaaggcg cacaaggaaa gcgacaagct catcgtgcgt cgccgtcgtt ctggcaagaa
     7921 gcgctgatag gagcctggaa tgccgcgtag cctcaaaaag ggccccttca tcgacgatca
     7981 ccttcagaag aaggtggacg tccagaacga agcaggcacc aagaatgtca tcaagacctg
     8041 gtctcgccgg tcggttgtca gccccgcgat gctcggtcac acgttcgccg ttcacgacgg
     8101 tcgcaagcac atcccggtct tcgtgaccga gtcgatggtc ggccacaagc tcggcgagtt
     8161 cgccccgacg cgcactttca agggtcacgt caaggacgac aagaagggtc gtcgtcgctg
     8221 atggccgcca acgagaagga cgtcaacacc gtggaagcca aggccgtcgc ccgccacgtg
     8281 cgggtcacgc ccatgaaggc gcgccgggtc atcgacctca ttcgtgggaa gaacgccacg
     8341 gacgccgtcg cgaccctgat gttcgcccag caggccgcct ccgagccggt tcgcaaggtg
     8401 ctgcagagcg ccatggccaa cgctcgggtc aaggccgacg ccgcctccga ggccttcgac
     8461 gagcgcctgc tcgtcgtctc ggcggcatac gtcgacgaag gggccaccat gaagcggttc
     8521 cggccgcgtg cgcaaggccg cgcctcgcgc atcaacaagc ggacgagcca catcacggtt
     8581 gtggtcacgc agcccgagat cgccaccagg aagggaaccc gctgatggga cagaaggtca
     8641 acccgcacgg cttccgcctg ggcatcacga ccgagcacaa gagccgttgg ttcgccgact
     8701 cgaccaagac gggtcagcgc taccgcgact acgtcaagga agacgtcgag atccgtcgcc
     8761 tcatggaaaa gggcatggag cgcgccggca tctcccgcgt ggagatcgag cgcacccgtg
     8821 accgagtccg tgtcgacatc cacaccgcgc g
//
LOCUS       DAOMZY010000129         8785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_7534_length_8785_cov_5.476518, whole genome shotgun sequence.
ACCESSION   DAOMZY010000129 DAOMZY010000000
VERSION     DAOMZY010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 8785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8785
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7534_length_8785_cov_5.476518"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1348
                     /locus_tag="PLK64_08655"
     CDS             <1..1348
                     /locus_tag="PLK64_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HPV79857.1"
                     /translation="TVEVGLTQRVRALERFLDDVYGPGDLFNDEVMPRTVVTSSPHFH
                     RVVAGLTPANGVRVHVSGIDLVRDGDGRFRVLEDNVRIPSGVSYVMTNRRALTSALPE
                     VFADHRIRPVRSYPARLLAALRASAPSGVSDPTVVVLTPGVYNSAYFEHALLARMMGC
                     RLVEGRDLVCQAGRVMVRTTTGLRPVHVIYRRVDDEFLDPVHFRGDSLLGCAGLVNAA
                     RAGNVTLANALGNGVADDKLVYTYVPDLIRYYLGEEPVLPNVDTWRLEDPAHRAEVLD
                     RLAELVLKPVDGSGGKGIVIGPAATSAQLDAVRARIQANPRGWIAQPVVQLSTVPTLV
                     GGQLAPRHVDLRPFAVNDGSSVWVLPGGLTRVALGEGQLIVNSSQGGGSKDTWVLANH
                     EVGDIEPIPAPRARPAAASSVVHPQARSVAIQHSEHSQVEQQQQQQQQRAHQEDPC"
     gene            1342..2265
                     /locus_tag="PLK64_08660"
     CDS             1342..2265
                     /locus_tag="PLK64_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HPV79858.1"
                     /translation="MLSRIAESLYWIGRYLERAEDTSRLLDVQLHLSVEDPVIDARAS
                     ADRLLTVMGIDHEGPATEDLVLRRLCHDPDSPASIVSALKGARESARRSRETVSTEMW
                     EAINTTWYAVQRGELAKVRPALAFRWVRERCAVITATADGTMSHDQGWYFLVLGRSLE
                     RIDMTARLAASAATGPAATAWSSTLRACGAHHAFVRGHHDQDSDSEAAEFLLLDRLFP
                     RSIVHTLDTAMDALDRLTPPERRGLTEDEVARTLGRARAELEYRTRGEVLLDLPERMA
                     ELQITCARANAAISARYFEGAVAAEWRGGAQ"
     assembly_gap    2362..2461
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2464..3309
                     /locus_tag="PLK64_08665"
     CDS             2464..3309
                     /locus_tag="PLK64_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HPV79859.1"
                     /translation="MSATIRIVHRTGYSYAGGVTASFNEIRMLPRSTHEQQVVHSRID
                     ISPVPWTYTYTDYWGTTVTAFEVFERHESLNVVATSTVDVQRHTSEEPRLQWGDLTSL
                     DVTDTHCEMLEIGDRVAPPPDLLAAVAELRRTSATPWELAVAVVDLVHAEVKYVFGAT
                     GVHTRASEAWEQRSGVCQDMAHLVLGALRSVGIPARYVSGYLMPSRTGELGVPYAGES
                     HAWIQFWDDGWVGADPTNNIRPGDLHVEVAVGRDYRDVAPLTGIFTGSRTSDMFVSVE
                     MTRLS"
     gene            complement(3311..3646)
                     /locus_tag="PLK64_08670"
     CDS             complement(3311..3646)
                     /locus_tag="PLK64_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79860.1"
                     /translation="MRRRADGLDVLVVYVPRASTEALLAALFAAGAGAIGDYRECAWV
                     TSGLGRFRPLPGANPAIGDVGELVTIEEDRCEVVIPTGRRDEVIAALRAAHPYEEPAF
                     HVIETAPLD"
     gene            complement(3643..4422)
                     /locus_tag="PLK64_08675"
     CDS             complement(3643..4422)
                     /locus_tag="PLK64_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPV79861.1"
                     /translation="MGEFVRLEVADGIGTIRLDRPKMNALNLQVQTEIEECAREATVR
                     DDVAAVIVYGGERVFAAGADIKEMQSMSYTDMAIAVEGLHSFTTTLARMPKPSVAAIT
                     GYALGGGCELAMCCDFRLAGESAQLGQPEILLGIIPGAGGTQRLTRLVGPAKAKDLIY
                     SGRFIKADEALAIGLVDRVVPDDQVYAAAREWMSQFVGGPRFALKAAKEAIDRGGELD
                     LDSGLALEKSLFSALFATHDRFVGMESFVVNGPGKAQFEGR"
     gene            complement(4415..4891)
                     /locus_tag="PLK64_08680"
     CDS             complement(4415..4891)
                     /locus_tag="PLK64_08680"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HPV79862.1"
                     /translation="MTDPTALPNFPPPSDEHPLRGRVLDALQDLGIEPVIDSDGDVAF
                     QVGEPSQQLFVRCQDGDFPVMRIFGQWQIGAPAPEDVLARLMRCNEVNLEWQVAKCGV
                     AGDNLIVTCDQVVMPGADLGQLMQLSVTLVLQVVGAWYGSWDQGRDEAPGAGGSDG"
     gene            4926..7073
                     /gene="glgX"
                     /locus_tag="PLK64_08685"
     CDS             4926..7073
                     /gene="glgX"
                     /locus_tag="PLK64_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HPV79863.1"
                     /translation="MLHRSLTSRDHPPPPGVTLVEGGAQVCVYAGHADGVQVCLFEPG
                     DIHGETERRVPLTERVYGYWFGFVPGMGVGQRYGFRVSGPWAPHDGMRYNPAKLLLDP
                     YARAIEGEVTWVPEVYGHRTSSDLRPEDLYAIDERDSAAYVPRSVVVDGGFDWGDDRP
                     LRRPLTESVIYETHVRNLTMRLPGVPANLRGTYAGLAHPVTIDYLTRLGITAVELLPV
                     HTFTTEPEVWRRGLINHWGYNTLGFFAPHARYAAARDPQGALDEFKGMVKLLHAAGIE
                     VILDVVYNHTAEQSARGATLSWRGLDNRAYYRLDGRGDDIDVTGCGNTVDVRHVVAVR
                     MILDSLRYWVQECHVDGFRFDLAVALGRGKNDEFDHDHPLLIALRTDPVLSQVKNIAE
                     PWDLGIQGWRTGQFPPLFSEWNDRFRDRVRSFWLTDLARNLAGQPGHGVQDLATRLAG
                     SADLFHHRDRGTIASINFVAAHDGFTTADLTAYNHKHNEANREDNRDGADNNSSWNHG
                     IEGQIDDGVSMLRHRSIRNLLATLLVSTGVPMLNGGDEFGRSQHGNNNAFCQDNDTSW
                     YDWDLAPWQADLLATTQFLIQLRAQHPVLRQRDFFSGGPVHLDGSKDLEWYGADGHPM
                     TMDRWGDARVRTLQMLLDGAHHGHQSLLVVFHGDAHEGTVTLPRPPGLTAYELLWDSA
                     WDRPPAQAAWVSPDEPAVLLPASMRIYRAVDPT"
     gene            7350..8312
                     /locus_tag="PLK64_08690"
     CDS             7350..8312
                     /locus_tag="PLK64_08690"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HPV79864.1"
                     /translation="MTSTSTEPAARRTPIDLRLDRPFRRRDALLAGLTDEDLRGPRFR
                     RLLHGIYISAAVEITPRIRVLAAMRTTTHPCFASHHTAATLLGATVPDSAWTHLGTTE
                     GAMSVRRQVKLRRYAVPPALAPQSPLPCTDAAQTFLDLARCLELVDLVVLGDGLVRQG
                     HITAPALLKAARQHEGPGAALARLAAGYVRPGVDSPMETRVRMLFVLAGFPEPVVNVE
                     VFDDAGRLRYRIDLAWPSAKVAVEYDGRHHINRQDQWTYDIRRREELEAEGWRFLVLT
                     APDIYRTPGTTLDRAESLLAAGGLRVRRRPDWFAHFPSQPSLSA"
BASE COUNT         1346 a         3183 c         2827 g         1329 t
ORIGIN      
        1 gacggtcgag gtggggctga ctcaacgggt gcgggccctc gagcggttcc tcgatgacgt
       61 ctacgggccg ggcgacctct tcaacgacga ggtgatgccg cggaccgtgg tgacgagttc
      121 gccgcacttt catcgggtgg tcgccgggct gaccccggcc aatggggttc gggtgcacgt
      181 cagcggcatc gaccttgtgc gcgacggcga cggccggttc cgggtgctcg aggacaacgt
      241 gcggatcccg tccggggtct cctacgtcat gaccaaccgc cgggccctca ccagcgccct
      301 gcctgaggtc ttcgccgacc accggatccg cccggtccgc agctaccccg cccgtctgct
      361 cgccgcgctg cgggccagcg cgccgagcgg cgtgtcggac ccgaccgtcg tcgtgctcac
      421 cccgggcgtc tacaactccg cctatttcga gcacgccctc cttgcccgga tgatgggttg
      481 tcggctcgtc gagggacgcg acctggtgtg ccaggccggg cgggtcatgg tccgcacgac
      541 gaccgggctg cgtccggtcc acgtcatcta tcgaagggtc gacgacgagt tcctcgaccc
      601 ggtgcacttc cgcggcgact cgctgctggg ctgcgcagga ctggtcaacg cggctcgcgc
      661 cggcaacgtc accctggcca acgccctggg caacggcgtc gccgacgaca aactcgtcta
      721 cacctacgtc ccagacctca tccgctacta cctgggcgag gagccggtgc tgcccaacgt
      781 cgacacctgg cgtctggagg acccggccca ccgggcggag gtcctcgacc gcctcgcgga
      841 gttggtcctc aagccggtcg acggctcggg cggcaagggc atcgtcatcg gtcctgcggc
      901 gacatccgct caactcgatg ccgttcgggc ccggatccaa gccaaccccc gcgggtggat
      961 cgcccagccc gtcgtgcaac tctcgacggt gccgacgctc gtcggagggc agttggcgcc
     1021 tcgccacgtc gacctgcgtc ccttcgcggt caacgacggg tcgtccgtgt gggtgctgcc
     1081 cggagggctg acccgcgtcg ctctcggcga gggccagctc atcgtcaact ccagccaggg
     1141 cggcggttcc aaggacacct gggtgctggc caaccacgag gtcggcgaca tcgagccgat
     1201 cccggcaccc cgcgcccgtc cggcggcggc gtcatcggtc gtccatccgc aggcccgcag
     1261 cgtcgccatc caacactccg agcactccca ggtcgagcaa cagcaacagc aacagcaaca
     1321 gcgcgctcac caggaggacc catgctgagc cgcatcgccg agtcgctcta ctggatcggc
     1381 cgctacctcg agcgggcgga ggacacctcg cgccttctgg acgtccagtt gcacctgtcc
     1441 gtcgaggatc ctgtcatcga cgcccgggcg tccgccgatc ggttgctcac cgtcatgggg
     1501 atcgaccacg agggccccgc gaccgaggac ctcgtgctgc gtcgcctctg tcatgacccc
     1561 gactcacccg cgtcgatcgt ctcggccctg aagggcgccc gcgagagcgc ccggcgctcc
     1621 cgtgagaccg tgtccaccga gatgtgggag gcgatcaaca ccacctggta tgccgtgcag
     1681 cgcggcgagc tcgcgaaggt gcgccccgcg ctcgcgttcc ggtgggtgcg ggagcggtgc
     1741 gccgtcatca cggcgaccgc cgacggcacg atgtcccacg accaggggtg gtacttcctc
     1801 gtcctgggcc gcagtttgga acgcatcgac atgacggcac gtctcgcggc gtccgccgcc
     1861 accgggcctg cggccactgc gtggtcgagc acgttgcgcg cctgcggggc ccaccacgcc
     1921 ttcgtgcgcg gccaccacga ccaggactcc gacagtgagg ccgctgagtt cctcctgctg
     1981 gaccggctct tcccgcgctc gatcgtccac accctcgaca ccgccatgga tgccctcgat
     2041 cggctcaccc cgcccgagcg tcgcggcctg accgaggacg aggtcgcccg caccctgggt
     2101 cgggcccgcg ccgagctcga atatcgcact cgcggagagg tgctcctcga cctgccggaa
     2161 cggatggccg agttgcagat cacctgcgcc cgcgccaacg ccgcgatctc ggcgcgatac
     2221 ttcgagggtg ccgttgccgc cgaatggcgc ggtggagccc agtgagcggg aatgcagtga
     2281 gcaggaatgc agtgagcggg agagtgcagt gagcgccacc atccgcatcg tccaccgcac
     2341 tggctattcg tatgccggtg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 ncagtgagcg ccaccatccg catcgtccac cgcactggct attcgtatgc cggtggtgtc
     2521 accgcgtcgt tcaacgagat ccgcatgctc ccccgctcca cccacgagca gcaggtcgtg
     2581 cacagccgga tcgacatctc gccggtgccg tggacctaca cctacaccga ttactggggc
     2641 accaccgtca cggcgttcga ggtgttcgag cggcacgagt cgctcaacgt cgtcgcgacg
     2701 agcaccgttg acgtgcaacg gcatacctcc gaggagccgc gccttcagtg gggcgacctg
     2761 accagcctcg acgtcaccga cacgcactgc gagatgctcg agatcggcga ccgggtcgcg
     2821 cctccgcccg acctcctcgc tgcggttgcc gaactgcgcc ggaccagcgc cacgccctgg
     2881 gaactcgccg tggcggtcgt cgatctcgtg cacgccgagg tgaagtacgt cttcggtgcc
     2941 accggagtcc acacccgggc ctcggaggcc tgggaacagc gctccggggt ctgtcaggac
     3001 atggctcacc tcgtgttggg ggccctgcga tcggtgggca tcccggcccg ctacgtgtcg
     3061 ggctacctca tgcccagccg cacgggcgag ctgggcgtcc cctatgccgg ggagtcgcac
     3121 gcgtggatcc agttctggga cgacggctgg gtgggcgccg acccgacgaa caacatccgc
     3181 cccggcgacc tgcacgtcga ggtggccgtc gggcgcgact atcgcgatgt ggcgccgttg
     3241 accggcatct tcaccgggag ccggacctcg gacatgttcg tctccgtgga gatgacccgg
     3301 ctgtcgtagg tcagtccagc ggggcggtct cgatgacgtg gaaggccggc tcctcgtagg
     3361 ggtgggccgc tcggagggcg gcgatcacct cgtcgcgacg acccgtgggg atgaccacct
     3421 cgcagcggtc ctcctcgatc gtgacgagtt ccccgacgtc gccaatggcg gggttggcgc
     3481 caggcagggg ccggaaccgg cccaggcccg acgtcaccca ggcacactcg cgatagtcac
     3541 cgatcgcgcc agctccggcc gcgaagaggg cagccagcag cgcctcggtc gacgcccgcg
     3601 ggacatagac gaccaggacg tccaggccgt ctgcgcgccg tctcaccggc cttcgaactg
     3661 cgccttgccc ggcccgttga cgacgaagga ctccatgccg acgaaccggt cgtgggtggc
     3721 gaacaaggcc gagaagagcg acttctccag ggccagcccg gagtcgaggt cgagctcgcc
     3781 acctcggtcg atggcctcct tggccgcctt gagagcaaac cgcggaccac cgacgaactg
     3841 tgacatccac tcgcgcgcgg cggcatacac ctggtcatcc gggaccaccc ggtcgacgag
     3901 cccgatcgcg agtgcctcgt cggccttgat gaagcgaccg gagtagatga ggtccttggc
     3961 cttggccggg ccgaccaggc gggtgaggcg ctgggtgccg cccgcgccgg ggatgatgcc
     4021 cagcaggatc tcgggctggc ccagttgggc cgactcgccg gcgagccgga agtcgcagca
     4081 catcgccaac tcgcagccgc cgcccagggc atagcccgtg atcgcggcga cgctcggctt
     4141 gggcatccgg gccagcgtcg tggtgaagga gtgcaggcct tcgaccgcga tcgccatgtc
     4201 ggtgtaggac atcgactgca tttccttgat gtcggcgccg gccgcgaaga cccgctcacc
     4261 gccatagacg atgaccgccg cgacgtcgtc acgcacggtc gcctcgcggg cgcactcctc
     4321 gatctcggtc tgcacctgca ggttcagcgc gttcatcttc ggacgatcca gccggatcgt
     4381 tccgatgcca tcggcgacct cgagccgcac gaactcaccc atcactgcct cctgctcccg
     4441 gcgcctcgtc gcgcccttga tcccacgatc cgtaccacgc gccgacgacc tgcagcacga
     4501 gcgtcaccga caactgcatg agttggccga ggtcggcccc aggcatgacg acctggtcac
     4561 acgtgacgat gaggttgtcg ccggcgacgc cgcacttggc gacctgccac tcgaggttga
     4621 cctcgttgca gcgcatcagg cgggccagga cgtcctcggg cgccggcgct ccgatctgcc
     4681 attggccgaa gatccgcatc accgggaagt ccccgtcctg gcagcggacg aacagttgtt
     4741 gcgagggctc gccgacctgg aaggcgacgt cgccgtcgga gtcgatgacc ggctcgatgc
     4801 ccaggtcctg cagggcgtca agcacccgcc ctcgcagcgg atgctcgtcc gagggcggcg
     4861 ggaaattggg cagggcggtg ggatccgtca tgccacaacc gtagtgtcca cctaggctgg
     4921 agggcatgtt gcatcggtct ctcacgagtc gcgaccaccc gccgcccccg ggagtgacac
     4981 tggtcgaggg cggcgcccag gtgtgtgtgt atgccggtca cgccgatggc gtgcaggtgt
     5041 gcctcttcga gccgggagat atccacggcg agaccgaacg ccgggttccc ctgaccgagc
     5101 gcgtctatgg ctactggttc gggttcgtgc ccggcatggg cgtgggtcag cgctatgggt
     5161 tccgggtctc cgggccctgg gccccgcatg acgggatgcg ctacaacccg gccaaactgc
     5221 tgctggatcc ctacgcccgc gctatcgagg gcgaggtcac ctgggttcct gaggtctacg
     5281 gccaccgcac gtcctcggac ctgcgccccg aggatctcta cgcgatcgac gagcgggact
     5341 ccgcggcata cgtcccgcgt tcggtcgtcg tcgacggagg tttcgattgg ggcgacgacc
     5401 gcccgctgcg acggccgttg accgaatcgg tcatctacga gacgcatgtg cgcaacctca
     5461 cgatgcgcct gcccggagtg ccggcgaacc tgcgcggcac gtatgccggt ctcgcccacc
     5521 ccgtgaccat cgactacctc acgcggctcg ggatcacggc ggtggagttg ttgccggtgc
     5581 atacgttcac gaccgagccg gaggtgtggc gccgcgggct gatcaaccac tggggctaca
     5641 acacgctcgg cttcttcgcc ccccacgcga ggtatgccgc cgcgcgcgac ccacagggcg
     5701 ccctcgacga gttcaagggc atggtcaaac tgctgcacgc ggccggcatc gaggtcatcc
     5761 tcgacgtcgt ctacaaccac accgccgagc agtcggcgcg cggcgcgacg ctgtcctggc
     5821 gaggcctgga caatcgcgcc tactaccggc ttgacgggcg cggggacgac atcgacgtca
     5881 ccggctgcgg taacaccgtc gacgtgcgcc acgtcgtcgc ggttcggatg atcctcgact
     5941 cgctgcgcta ctgggtgcag gagtgccacg tcgacgggtt ccgcttcgac ctggccgtcg
     6001 cgctcggccg cggcaagaac gacgagttcg accacgatca cccgttgctc atcgcgctgc
     6061 gcaccgaccc ggtgctctcg caggtgaaga acatcgccga gccgtgggac ctgggcatcc
     6121 agggctggcg gacggggcag ttccccccac tgttctctga gtggaacgac cggttccgcg
     6181 accgggtgcg ctccttctgg ctcactgacc tcgcccgcaa cctcgccggt cagccggggc
     6241 acggggtgca ggacctggca acccggctcg ccggatcggc cgacctcttc caccaccgtg
     6301 accgaggcac gatcgcctcg atcaatttcg ttgcggcaca cgacggtttc acgacagccg
     6361 acctcacggc atacaaccac aagcacaacg aagccaaccg cgaggacaac cgcgacggcg
     6421 ccgacaacaa ctcctcgtgg aaccacggca tcgaaggcca gatcgacgac ggcgtctcga
     6481 tgctgcggca ccgctcgatc cgcaacctgt tggccaccct gctcgtgtcg accggggtgc
     6541 cgatgctcaa cggcggcgac gagttcggtc ggagccaaca cggcaacaac aacgccttct
     6601 gtcaggacaa cgacacctcc tggtacgact gggatctcgc cccctggcag gccgacctgc
     6661 tggccacgac gcagttcctc atccagttgc gggcccaaca cccggtgttg cgccagcgcg
     6721 acttcttcag tggcgggccg gtccatctcg acgggtccaa ggacctcgag tggtacggcg
     6781 ccgacggaca tcccatgacg atggatcgct ggggtgacgc tcgcgtccgg accctgcaga
     6841 tgcttctcga cggcgcccac cacgggcacc agtcgctgct cgtggtcttc cacggcgatg
     6901 cccacgaggg gaccgtgacc ctgccgcgcc cacccgggct cacggcatac gaactgctgt
     6961 gggacagcgc ctgggaccgg ccgcccgctc aggctgcctg ggtgtccccc gacgagcccg
     7021 ccgtcctgct gccggcctcg atgcgcatct accgcgccgt cgacccgacc tgacaccctc
     7081 cccttcccac cccctgccca ccccctgccc accccttccc gccccccgcc ccccttttcc
     7141 caaattcctc acactcgccg gcgatgtggg cctccacagg ccttctgccc cgacttcgcc
     7201 acacccgccg gcgatgtgtt cgcgccgcct tggtgaccgc gtctgacgcc ccacgagcgc
     7261 gccgccgccc cctgtggata actcctgcac ccactcccca acctcgggca ttgtcgtcag
     7321 gagccaccgc gaccgcagga gtgtggacct tgacctcaac cagcaccgag cccgccgccc
     7381 ggcggacccc gatcgacctc cgtctcgacc gcccgttccg ccgtcgagac gcgctgctcg
     7441 cggggctcac cgacgaggac ctcaggggcc cgaggttccg tcgattgctg cacggcatct
     7501 acatcagcgc ggcagtcgag atcactccgc ggattcgagt gctcgccgcg atgcgcacga
     7561 cgacgcaccc ctgcttcgcc tcccatcaca cggccgccac cctcctcggg gcgaccgtgc
     7621 cggacagcgc gtggacccac ctcgggacaa cggagggcgc tatgagcgtg cgccgccagg
     7681 tcaaactgcg ccggtatgcc gtcccgccgg ctctcgcgcc ccagtcaccc ctgccgtgca
     7741 cggacgccgc gcagacgttc ctcgacctgg ctcgatgtct cgagctcgtc gacctcgtgg
     7801 tcctaggcga cgggctggtc cgccagggtc acatcaccgc gcccgcgctc ctcaaagcgg
     7861 cacgccagca cgaaggtccc ggcgcggctc tcgcgcggct ggctgccgga tacgtgcgcc
     7921 ccggggtgga ctctcccatg gagacccggg tgcgcatgct cttcgtgctg gccggcttcc
     7981 ccgaacccgt ggtcaacgtc gaggtgttcg acgacgcagg caggctgcgc tatcggatcg
     8041 acctggcctg gccgtccgcg aaggtcgccg tcgaatacga cgggcgtcac cacatcaacc
     8101 gacaggatca gtggacctac gacatccgcc gccgggaaga gttggaggcg gagggctggc
     8161 ggttcctcgt gctgactgct cccgacattt accgcacgcc cgggacgacg ctcgaccgcg
     8221 cggagagcct cctcgcggct ggcggcctcc gagtccggcg gcgccccgac tggttcgccc
     8281 acttcccgag ccagccgagc ctctcggcct gacgcgtcga cgcctcgacg cctcaacgtc
     8341 cggggctggt ccgggcggat gccggacccg tgaatccgcc ccgagcctgg atctgcccag
     8401 atcaccggcg ggtgtgagga attcggggcc aaccggcata cccctccccc agaccaccgg
     8461 cgagtgtgag gaccttggga acatccgagc cccgacccgc ccagaccacc ggcgggtgtg
     8521 aggaattcgg ggccaaccgg catacccctc gcccagacca ccggcgagtg tgaggaaatc
     8581 ggggccaacc ggcatacccc tccgcccaga tcaccggcga gtgtgaggaa atcggggcca
     8641 accggcatac ccctccccca gatcaccggc gagtgtgagg aaatcgggaa aaggtcggca
     8701 gggaacgcgg gggcgggggc cagagctgga aacgcagtcg cgcggcatac cccctgagga
     8761 ggtatgccgc gcggctggcg gtgct
//
LOCUS       DAOMZY010000130         8762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_7563_length_8762_cov_5.905478, whole genome shotgun sequence.
ACCESSION   DAOMZY010000130 DAOMZY010000000
VERSION     DAOMZY010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 8762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8762
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7563_length_8762_cov_5.905478"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PLK64_08695"
     CDS             complement(<1..409)
                     /locus_tag="PLK64_08695"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HPV79865.1"
                     /translation="MAADPLTVARPFQRDLLIVIGLAASPLVALGCSRFAYALLLPAM
                     RSDLGWSFTTAGLMNTVNAVGYLVGALGTAWAAAKLGQRPAFVGSLVITVLALAATAA
                     SSSLEVLLAVRTILGIAGAGAFVLGGAITSTISR"
     gene            complement(416..6622)
                     /locus_tag="PLK64_08700"
     CDS             complement(416..6622)
                     /locus_tag="PLK64_08700"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4011 domain-containing protein"
                     /protein_id="HPV79866.1"
                     /translation="MAPLTHLEVVTAPLVNHAMAHNGIRVVRQITLVSSPDLDGGHTD
                     VVLTARLVDAHSTVLTRPWQHHVDRLDAGGRVRLDNPDITLDPAAIASLDEETGADLI
                     VEATSAGQTFVTSTTPMRVLAARQWLVDPDAPVLSLELLAAFVQPNHPALGPLVARAA
                     AYLEAETTSGSLAVDHVLPGRIDAIVEAVCAAVHASGIYYAQPPASWGYGQKVRTPGD
                     VLEQKVGTCLDTTLLVASALEHLGIHPVLWIVRGHAFLGYWRTTDTGLPDAASLQCAQ
                     AVNAVGLGLMGVVETTMLTQERRPPSDLFRRASQAPRDTYLHGDPAALIGVVDVYLAR
                     LMRVFPMPARRIREDGVVEVIEYRPPTAPTDSVAAQRDSQAPPAQSGIPAPTAATPRR
                     PEPPPRVQAWKNSLLDLTLRNRLLNSARGVTQLPLLMPGEHLGALADLLTAGTPVVVR
                     AADDLSGAVLAQEVRDAYGLPADVLRTMLRDKATVYAAADGETHRLMVARLRYRARTG
                     LQETGANPLALTLGRLDWRLGDRELTAPLLLAPVELKGIVQPFKIVPDETAGLTLNLS
                     LMEKLRIEFGFVVPGLDELPVRPAGVRGEGGVDVDEVVRRLRQALLDAGLPFTVETEA
                     RLAIVGFTGYLLWRDLNEHWERFLTQPVARHLALTPTDRFTDPAAAEVDTSLATLDGV
                     VADVPIPADGSQAEAIAFARGGRTFVLEGPPGTGKSQTITNILADQVARGRRVLFVAE
                     KGAALDVVRRRLAEVGLAPFALDLHDEHATPAQVRERLRLAIAHVPRPDQAGFQAAAT
                     DVSSSAATLASYAARLHSPGGAGLSAYSAQAQLLALGIGPIAPVPPLVVRVRSDPPTG
                     YDVGAPPLEIDACRRAVAAATGLLAGLGSTGFDAWGFASAMPADVVRLFSLVEDADLA
                     VAELERRLAGCSEPVRAALATAIIPDDLERIGWLLTNHAVSPEVLDETRSRRWREARD
                     ELEARQTHLDTLAAPVLAVLTPEALSVPVEPIRQAVREAASSFFIGRKGRLLHAAAPL
                     LVHALPGADIPPKTLPTLVEQVAAVAGETASLAHAWHSLPGLGVVPPDANLLAPQSRS
                     ALTDALTAIDRDFAILDRLMSPTANAVREARDNGEVLLDEAYAALRAASSCVAAVMAA
                     TGTPVGTVNGLHRLWAEHNVRGVLKAWQAGNRARLADRGAGATLRQWIEAVTALEPLD
                     RSGLSMAQEAILSGEVPASGAVAALDRGLAGAALEERLGTRAMAGFDGEAHDRIVDRF
                     VGASDALRGTLRGILPSRVVQERPFKPGATFGTVAALEREVGRTRGGLSVRRLVQTYG
                     EVVGELTPCVLVSPDSLARFIPPGSIDFDLVVFDEASQITVPDAIGALGRARAAVIAG
                     DSKQMPPSSFGEVGWDDSAESDRDLPVASVAPVTAVAADPSAADGGEPEATAADFRIV
                     PDEESILSEMVHAGVDRLWLSWHYRSQDESLIAFSNGHYYDNRLSSFPAYPGEVTDTG
                     LSFTRVDGTFYRTSSRAARGPGGPGGASPGGPLRTNPVEAAAVVEEVRRRWGQGERSI
                     GVVTFNLQQRTLIEKLLWDLGDETIADSLASGKDGLFVKNLENVQGDERDVIIFSTGF
                     AKTASGVLPLNFGPLNRTGGERRLNVAITRARRRVMVFSSFEPEDLRVEQTSSVGVQH
                     LRAYLEVAKYGVGHAPTGGGGLVGTAAGLLSDGDRSAPRGRIDAVDRHKDDVAAALRA
                     EGLSVTVGVGLSDFKVDLAVGPPGKPPVLAILLDGPEWAERRTTNDRDGLPTTVLQHI
                     MGWPMVMRIWLPSWLFSRDDVVATVCERAEIASRLPRHVGERRVSTGYAAAAPPQDGQ
                     PLAGAGPQGGAGAQGGAGPQDGQPPAGARPNSVPTPAAAPESYAPFIPRQEGGLSVLD
                     GVKRRSPRHAAAVQDVMRRIVAEEGPLSEARLFLLTAQCFGMTRPSVYRVRDMAAIIP
                     SDLRRDPEERFVWPDERDPLRWSGFRTWEGVLKERPLEEIPLRELANALVAIARSAMG
                     IDLDELLKQTLTVFNGTRLTEVPRRRLMAALQVAQDRGQLNVSGDIVTATDG"
     gene            6665..7042
                     /locus_tag="PLK64_08705"
     CDS             6665..7042
                     /locus_tag="PLK64_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79867.1"
                     /translation="MKLFNEIIVAFHLLGMAAIVGGWLAVRHDLKIVPAILWGARVQL
                     LTGLILAGFASMDKEDPPNNAKLGVKLLVALVVVALSESTWAAQKKAVAGAAQAAVAS
                     KVNLVGGLAVLNVLIAVLWRSYS"
     gene            complement(7137..7877)
                     /locus_tag="PLK64_08710"
     CDS             complement(7137..7877)
                     /locus_tag="PLK64_08710"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HPV79868.1"
                     /translation="MISVGNTQRSYRVYRPQGITTAAPLVMVFHGYTWNAARTEDTFG
                     WNMLADAQRFIVLYPEGLNQGFNAGGCCGISSDTHVDDVSAALAMVDDVAKTIALDTD
                     RLFVTGFSNGGAMTYRMACETDRFAAFGPVAGGLVVDCKDGKPASILHLHGLADTDVP
                     VVGDSRIWRTPVDQVLADWRTRDDCAAPGAPTTSGTSHRVTATCAAGREVGLITIDGL
                     RHAWPTAADGLDATSTLWAFFAKHPRGH"
     gene            complement(8095..>8762)
                     /locus_tag="PLK64_08715"
     CDS             complement(8095..>8762)
                     /locus_tag="PLK64_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPV79869.1"
                     /translation="GMPGMDYRLGTATDGGMVSGMMALEGGQAAPPPYWLIYFTTEDC
                     DARVAAITEAGGSVIAAPADIPGTGRFAVAADPQGAVFGLLQPLPMDAPAEHPAFDQQ
                     APGHGNWHELMSSDPEAGLAFYAEQFGWTKGDTMDMGEMGTYQIFGSGGQDLGGMMGL
                     GNSPVSAWLPYFGSTGIDQSIERIIAGGGTIHHGPAEVPGGAWIALATDPQGAWFAVV
                     GPR"
BASE COUNT         1348 a         2869 c         3270 g         1275 t
ORIGIN      
        1 acctgctgat cgtcgaggtg atcgcgccac cgaggacaaa ggcgcctgca ccagcgatgc
       61 cgaggatcgt gcggactgcc agcaggacct ctagcgaact ggacgcggcg gtcgctgcaa
      121 gagcaagcac ggtgatgacg agcgagccga cgaaggcggg gcgttggccg agtttcgctg
      181 cggcccaggc ggttccgagt gcacccacca ggtagcccac ggcgttgacc gtgttcatca
      241 gcccggccgt cgtgaacgac cacccgagat ccgaacgcat cgcgggcagc agtagtgcat
      301 aggcgaaccg cgaacaaccc agggccacga gcggggaggc ggcgaggccg atgacgatca
      361 acaggtcgcg ctggaaaggg cgtgccacag tgagcgggtc ggcggccatg accgcctagc
      421 cgtccgtggc tgtgacgatg tcgccgctga cgttcaactg cccacggtcc tgtgcgacct
      481 gcaaggcggc catcaggcgg cgccggggga cctctgtgag ccgggtgccg ttgaagaccg
      541 tcagggtctg cttgagcaac tcgtcgagat cgatgcccat cgccgaccga gcgatggcga
      601 ccaaggcgtt ggcgagctcg cgcagcggga tctcctcaag cgggcgctcc ttgaggacgc
      661 cctcccacgt gcggaaaccg ctccagcgca acgggtctcg ctcatcgggc cagacgaatc
      721 gctcctccgg atcgcgccgc aggtccgacg gaatgattgc ggccatgtcc cggacccggt
      781 agacgctggg ccgggtcatt ccgaaacact gggcggtcag caggaacagt cgagcctcag
      841 acagcgggcc ttcctcggcg acgatccggc gcatgacgtc ctggacggcg gcggcatgcc
      901 gtggactgcg acgtttaacg ccatcgagga cgctgaggcc accttcctgg cgtgggatga
      961 agggcgcgta ggactccggc gccgcggcag gggtgggaac actgttgggt ctggccccgg
     1021 ccggcggctg cccgtcctga ggtccggcac cgccctgagc tccagcacca ccctgaggac
     1081 cggcacccgc cagaggctgc ccgtcctggg gaggtgcggc ggcggcatac cccgtgctca
     1141 cccggcgctc accgacgtgg cgcggcaggc gagaggcgat ctcggcccgc tcgcacacgg
     1201 tggcgaccac atcgtcccga gagaacaacc acgagggcag ccagatccgc atgaccatcg
     1261 gccagcccat gatgtgctgc aggacagttg tggggaggcc gtcgcgatcg ttggtcgttc
     1321 ggcgctccgc ccactcgggc ccgtcgagca ggatcgcgag cacgggaggt ttcccgggcg
     1381 ggccgaccgc gagatcgacc ttgaagtcgg acagaccaac gcccacggtc acggacaggc
     1441 cctcggcccg cagggcggcg gcgacgtcgt ccttatgccg gtcgacggcg tcgatgcgtc
     1501 cgcggggagc tgagcggtcg ccgtcgctga ggaggccggc ggcagtgccg acgagcccgc
     1561 ctccgcccgt gggcgcgtgt ccgacgccat atttggccac ctcgagatag gcgcgcaggt
     1621 gttggacgcc gaccgacgag gtctgctcga cccgcaggtc ttcgggctcg aaggaactga
     1681 acaccatgac ccgacggcgc gcgcgggtga tggcgacgtt gaggcgccgc tcgcccccag
     1741 tgcggttaag cggcccgaag ttgagcggta ggacaccgga cgcggtcttg gcgaacccgg
     1801 tcgagaagat gatgacgtcg cgctcgtcgc cctggacgtt ctcgaggttc ttgacgaaca
     1861 ggccgtcctt gccgctggcc aggctgtcgg cgatggtctc gtcgccgagg tcccagagca
     1921 gtttctcgat gagagtgcgc tgttggaggt tgaaggtgac gacgccgatc gagcgctcgc
     1981 cctggcccca ccggcggcgc acctcctcga ccacggcagc agcctcgacg ggattggtcc
     2041 gcaacgggcc gccgggcgac gcgccgcccg ggccgcccgg gccgcgagcg gcgcgcgacg
     2101 aggtgcgata gaaggtgccg tcgacccggg tgaacgacaa ccccgtgtcg gtgacctcgc
     2161 ccggataagc cgggaaagag ctgagccggt tgtcgtagta gtgaccgttc gagaacgcga
     2221 tgagcgactc gtcctggctg cggtagtgcc acgacagcca cagccggtcg acgcctgcgt
     2281 ggaccatctc cgagaggatc gactcctcgt cgggcacgat ccggaagtcg gcagccgtcg
     2341 cctcgggctc gcccccatcc gcggcgctcg ggtccgccgc gaccgccgtg accggtgcga
     2401 ccgatgcgac cggcagatcc cggtcggact ccgcagagtc gtcccagccg acctccccga
     2461 aactcgatgg cggcatctgc ttggagtcgc ccgcgatgac ggcagcccgc gcccgtccga
     2521 gagcgccgat cgcatccggc accgtgatct gcgaggcctc gtcgaacacg accaggtcga
     2581 agtcgatcga ccctggcggg atgaagcgcg ccagggagtc ggggctgacg agcacgcatg
     2641 gggtgagctc cccgacgacc tcgccatagg tctgcaccaa ccgccgaacg ctcagccctc
     2701 ctcgcgtgcg acccacctcg cgttccaggg cggccacggt cccgaacgtc gctcccggct
     2761 tgaacggccg ctcctgcacc acccggctgg ggagtatgcc gcgcagggtc ccccgcagcg
     2821 cgtccgatgc cccgacgaag cggtcgacga tccggtcgtg cgcctccccg tcgaatccag
     2881 ccatcgctct cgtgcccagt cgttcctcca aggcggcacc cgccaagccc cggtcgagtg
     2941 ccgcgacggc cccggacgcg ggcacctcgc ccgacaggat tgcctcctgc gccatggaca
     3001 gaccggaccg gtccaggggt tcgagggctg tgactgcctc gatccattgg cgcaatgtgg
     3061 cacctgcgcc tctgtcggcg agccgcgccc ggttgccggc ctgccaggcc ttcagcacgc
     3121 cccggacatt gtgctcggcc cacagccggt gcagtccatt gacggtcccc acaggggtcc
     3181 cggtggcggc catgacggcg gcaacgcacg acgaggcggc gcgcagggcg gcatacgcct
     3241 catcgagcag gacctccccg ttgtcgcgcg cctcgcgaac cgcgttcgcc gtgggggaca
     3301 tcaaccggtc caggatggcg aagtctcggt cgatggcggt gagcgcatcg gtgagcgcgc
     3361 tacgactttg gggggcaagg agattcgcgt cgggtgggac tacgccgagc cccgggagag
     3421 agtgccaggc gtgcgcgagg gatgccgtct cgccggccac cgcagcgacc tgctcaacaa
     3481 gcgtaggcag ggtcttgggc gggatgtcgg cacctggaag ggcatgcacg agcagggggg
     3541 cggcggcatg cagcaggcgg cccttgcggc cgatgaagaa cgacgaggcc gcctctcgga
     3601 ccgcctggcg gatgggttcc acggggacgc tcagcgcctc gggagtcagg acggcgagca
     3661 cgggagcggc cagcgtgtcg aggtgggtct ggcgggcctc gagttcgtct cgggcctcgc
     3721 gccagcgtcg ggagcgggtc tcgtcgagga cctccgggga gacggcgtgg ttggtcagga
     3781 gccagccgat gcgctccagg tcgtcaggga tgatggccgt ggccagggcg gccctgacgg
     3841 gctcgctgca gccggccagc cggcgctcga gctcggcgac cgcaagatcg gcgtcctcga
     3901 cgaggctgaa cagcctgacg acgtcggcgg gcatggcgct ggcgaaaccc caggcatcga
     3961 atccggtgct gcccaggccc gcgagcaggc cagtggcagc ggcgacggct cgtcggcacg
     4021 cgtcgatctc cagcggtggg gcgccgacgt catagccggt gggcgggtcg ctgcggaccc
     4081 gcaccacgag gggagggacg ggggcgatcg gccctatccc gagagcgaga agttgggcct
     4141 gggcggagta ggccgacaaa ccggcgccgc cgggggagtg gaggcgggcg gcatacgagg
     4201 cgagggtggc ggccgaggac gaaacgtcgg tggccgcggc ctggaagccc gcctggtcgg
     4261 gccgaggcac gtgggcgatg gcgagccgca gccgctcacg gacctgcgcg ggcgtcgcgt
     4321 gctcgtcgtg caggtcgagc gcgaacgggg cgaggccgac ctccgccagc cgacgccgca
     4381 cgacgtcgag cgcggcgccc ttctcggcga cgaacagcac ccggcgcccc cgcgcgacct
     4441 ggtcggcgag gatgttggtg atggtctggg acttgccggt gcccggcggg ccttccagga
     4501 cgaaggtgcg cccaccgcgg gcgaaggcaa tcgcctcggc ctgcgagccg tccgcgggga
     4561 tgggcacgtc ggcgaccacg ccgtcgaggg tagccaggct cgtatccacc tctgccgcag
     4621 cgggatcggt gaaccggtcg gttggggtca gggcgaggtg gcgggcaacc ggctgggtga
     4681 ggaaccgttc ccagtgctca ttgaggtctc gccagagcag gtagccggtg aaaccgacga
     4741 tggccaggcg cgcctcggtc tcgacggtga agggcaggcc ggcatcgagg agggcttggc
     4801 gcaggcggcg cacgacctcg tcgacatcga cgccgccctc gccgcggacg cccgctggac
     4861 ggactggcag ctcgtccaat cccggcacca cgaaaccgaa ttcgatccgc agcttctcca
     4921 tcagcgagag gttgagcgtg aggccggccg tctcgtcggg gacgatcttg aagggttgga
     4981 caatgccctt gagctcgacg ggtgccagga gcagcggcgc ggtcaactcg cggtcgccga
     5041 gtcgccagtc cagccgcccg agggtgagcg ccagcgggtt ggctccggtc tcctgcagcc
     5101 cggtccgggc gcggtaacga agcctcgcca ccatgagccg atgggtctcg ccgtcggcgg
     5161 cggcatacac cgtggccttg tcgcgcagca tggtgcgcag gacgtcggcg ggcagtccgt
     5221 aggcgtcgcg gacctcttgg gcgagcaccg caccagagag gtcgtcagcc gcgcggacga
     5281 ccacgggggt gcccgcggtg agcagatcgg ccagggcgcc gaggtgctcg ccgggcatga
     5341 gcaggggcag ttgggtgacg ccgcgcgcag agttgagcag ccggttgcgc agggtgaggt
     5401 ccagcaggga gttcttccac gcttgcaccc gcgggggcgg ctcgggtcgg cgcggggtgg
     5461 ccgcggtggg agcgggtatg ccgctttgcg ccggtggggc ttgcgagtcg cgttgggccg
     5521 caacggagtc ggtgggcgcg gtcgggggtc ggtattcgat gacctcgacg acaccgtcct
     5581 cgcgaatgcg tcgcgcaggc atggggaaca cccgcatgag tcgggcgagg tagacgtcga
     5641 ccacgccgat cagcgccgcc gggtcaccgt gcagataggt gtcccgaggc gcttgcgacg
     5701 cgcgtcggaa caggtcgctg ggagggcgtc gctcctgggt gagcatggtc gtctcgacga
     5761 cacccatgag cccaagcccg accgcgttga cggcctgcgc gcactgcagg ctggccgcgt
     5821 cgggcagccc ggtgtcggtc gtgcgccaat agccgaggaa ggcgtggccg cggacgatcc
     5881 agaggaccgg gtggatgccg aggtgctcca gcgctgaggc gacgagcaac gtggtgtcca
     5941 ggcaggttcc gaccttctgc tccaggacgt cgccgggggt gcgaaccttc tggccatacc
     6001 cccacgaggc cggcggctgg gcgtagtaga tgcctgacgc gtggacggcc gcgcacaccg
     6061 cctcgacgat cgcgtcgatc cggccaggca ggacgtggtc gacggccagt gagccggagg
     6121 tcgtctccgc ttccaggtat gccgccgccc gcgccacgag ggggcccagc gccgggtggt
     6181 tgggctggac gaaggcggcg agcaactcga gggacaggac gggggcgtcc gggtcgacca
     6241 gccattggcg ggccgcgagg acccgcatcg gcgtggtcga ggtgacgaag gtctggccgg
     6301 cgctggtggc ttcgacgatg aggtcggcgc cggtctcttc gtcgaggctg gcgatggcgg
     6361 ccgggtcgag ggtgatgtcc gggttgtcga ggcgcaccct ccctccggcg tcgaggcggt
     6421 cgacgtggtg ctgccagggc cgggtgagca cggtgctgtg ggcgtcaacg agccgggcgg
     6481 tgaggacgac gtcggtgtgg ccaccgtcca ggtcggggct ggagaccagg gtgatctggc
     6541 gcacaacgcg gatcccgttg tgggccatcg cgtggttgac cagcggcgct gtgaccacct
     6601 caaggtgggt caagggcgcc atggccagac tctagaccgt ggggagtgcg gatagcctga
     6661 cgccatgaag ctgttcaacg agatcatcgt cgccttccac ctgttgggca tggccgccat
     6721 cgtcggtggg tggctggccg tccggcacga cctcaagatc gtgcccgcca tcctgtgggg
     6781 cgcgcgggtt cagttgttga ccgggctgat cctcgcgggg tttgcttcca tggataagga
     6841 ggacccgccc aacaacgcca agctgggggt caagctcctg gtcgcgttgg ttgtcgtggc
     6901 gctgtcggag tcgacctggg cggcgcagaa gaaggctgtc gcgggcgcgg cccaggccgc
     6961 cgtggcctcg aaggtcaatc tggtcggtgg gttggcggtc ctcaacgtcc tgatcgccgt
     7021 gctctggcgc tcctacagct gagccagctt gccgggccga ggtattccac cgtcaccaga
     7081 cgatggccga atacctcgac ccggcgaggc ctgcgggtga ggcaggtgcc gaggagtcag
     7141 tgcccccgcg ggtgtttcgc gaagaaggcc cacagcgtgg acgtggcgtc gaggccgtcg
     7201 gccgccgtgg gccaggcgtg gcggaggccg tcaatggtga tgagaccaac ctcgcggccc
     7261 gccgcacagg tggccgtcac gcgatgggat gtgccactgg tcgtgggcgc tccgggggcg
     7321 gcgcagtcgt cgcgcgtgcg ccagtcggct agcacctgat cgaccggagt gcgccagatc
     7381 cgactgtcgc cgacgacagg gacgtcggtg tcggccagcc catggaggtg gaggatcgag
     7441 gcaggcttgc cgtccttgca gtcgacgacc aggccccccg cgacggggcc gaacgcagcg
     7501 aagcggtcgg tctcacatgc catccggtac gtcatggcgc cgccgttgga gaagcccgtg
     7561 acgaagaggc ggtcggtgtc gagcgcgatg gtcttcgcga cgtcatccac catggccaac
     7621 gcggccgaga catcgtcgac gtgggtgtcg ctggagatgc cacagcaacc accggcgttg
     7681 aatccctggt tgagcccctc ggggtagagc acgatgaacc gttgggcgtc agccaacata
     7741 ttccacccga acgtgtcctc cgttcgggcg gcattccacg tgtagccgtg aaagaccatc
     7801 accagtggtg ccgctgtggt gatcccttgc ggacggtaga cgcgataact gcgttgggtg
     7861 ttgccgaccg agatcacccg ttccgacgat ccctctgcca gagtcgctga agtcggcgtg
     7921 ggtgtcccag ctgagggggc ggacgggctg ccgcaggcag ccaccgcgag ggtgagcgcg
     7981 accaggagca gaccgagact ccacccccgt cgcggtcgac ggggatgggc ttcgtgtttg
     8041 gcggactggg ttgactcaga ggaactcatg tccgccttgg gtgggagggg ggaatcagcg
     8101 cggtccgacg acggcgaacc aggcaccttg ggggtccgtg gccagggcga tccaggcgcc
     8161 gccgggcact tcggccgggc cgtggtggat cgtgccgcca ccggcgatga tcctctcgat
     8221 cgactggtcg atccccgtcg agccgaagta gggcagccag gccgagaccg ggctgttgcc
     8281 cagtcccatc atgccgccga ggtcctgccc accactcccg aagatctggt aggtgcccat
     8341 ctcacccatg tccatcgtgt cgcctttggt ccagccgaac tgttcggcgt agaaggcgag
     8401 tccggcctcg gggtccgagc tcatcaactc gtgccagttc ccgtgtccgg gggcttgttg
     8461 gtcgaaggcc gggtgctccg ccggggcatc catcggcagc ggctgcaaga gtccgaagac
     8521 cgcgccctgc gggtcggcag cgacggcgaa ccggcccgtg ccggggatgt cggcgggggc
     8581 cgcgatcact gagccgccag cctccgtgat ggctgcgacc cgcgcgtcgc aatcttcagt
     8641 ggtgaagtag atcagccagt agggcggcgg tgcggcctgg ccgccctcca gggccatcat
     8701 gccgctcacc atgccgccat ccgttgcggt gccgagccgg tagtccatcc ccggcattcc
     8761 gg
//
LOCUS       DAOMZY010000131         8753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_7579_length_8753_cov_5.896413, whole genome shotgun sequence.
ACCESSION   DAOMZY010000131 DAOMZY010000000
VERSION     DAOMZY010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 8753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8753
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7579_length_8753_cov_5.896413"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..471)
                     /locus_tag="PLK64_08720"
     CDS             complement(187..471)
                     /locus_tag="PLK64_08720"
                     /inference="COORDINATES: protein motif:HMM:NF022668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3017 domain-containing protein"
                     /protein_id="HPV79870.1"
                     /translation="MTTETRSALRAVRRLGLWWVVGVIAGAGLLVVGLGQVRPGGYLL
                     ATACAVGGLLRAIRPLGRAGGLAVRRRSVDVVCWLGLGATVAVAFALVRL"
     gene            complement(468..1337)
                     /locus_tag="PLK64_08725"
     CDS             complement(468..1337)
                     /locus_tag="PLK64_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HPV79871.1"
                     /translation="MTAQILDGKATLTTVKAELAARVAALAERGIVPGLGTVLVGDDP
                     GSQWYVGAKHQDCAELGIRSIRRDLPATATQAEVEAVIDELNADPGCTGFLVQQPTGL
                     DEMALLSRVDPAKDVDGLHPMNLGWLVLGRPAPLPCTPMGVIELLRRFGVPIAGAKVC
                     VVGRGLTVGRPLGLILTRKSENATVTLCHTGTRDLAAEVRQADIVVAAAGVPNIITAD
                     MVKPGAAVLDVGVSRVTDPATGKSRVAGDVAADVAEVAGWVSPNPGGVGPMTRALLLI
                     NVVLAAERQAASA"
     gene            1602..2510
                     /locus_tag="PLK64_08730"
     CDS             1602..2510
                     /locus_tag="PLK64_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79872.1"
                     /translation="MPVLTVGTGQPTATATLTPTALPPPTPTPPPIPPPTPTLTLPGG
                     GTTVFPDRRLVGFSGYPGAPALGRLGVGNLDERGADITALAGEYAAGRRPLPVFELIA
                     TVVHSRPGADGLYRTRIDEGVVRRHLEAARRHGAILLLNIQPGRAAFLDEVKHWEPWL
                     REPDVGLALDPEWAVTAGQVPGKVFGRTTGAELDAIAAYVAGIVAANRLPEKVMVYHQ
                     LSRSIVREPSGLQPHPGVTLVVSVDGIGSRAQKAATWRSIVAETPDHVHKGFKLFYDE
                     DAQIGPLMTPAEVLALTPQPEYVLYE"
     gene            complement(2526..4181)
                     /gene="purH"
                     /locus_tag="PLK64_08735"
     CDS             complement(2526..4181)
                     /gene="purH"
                     /locus_tag="PLK64_08735"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HPV79873.1"
                     /translation="MSSVSAPGPTQDVRPVRRALVSVYDKSGLEDLVRGLADAGVELV
                     STGGSAALIAGLGLPVTKVEDLTGFPECLEGRVKTLHPRVHAGLLADTRKPDHLAQLA
                     ELGVEAFELVAVNLYPFAATVASGATPDECVEQIDIGGPSMVRAAAKNHPSVAVLTSP
                     AQYAAALAAVRSGGFTFAARQRLAAEAFAHTAAYDVAVASWMGSSAYADTSDGTGFAA
                     FAGATFTRAAGLRYGENPHQQAALYLSGAPGIASATQLHGKEMSYNNYVDADAALRAC
                     RDHGGLPTCAIIKHANPCGIAVGTEEGGIAQAHRRAHACDPVSAFGGVIAVNRPVTLE
                     MAETVKDIFTEVVVAPSFDDDALAVLTAKKNIRLLTVDFAALDAAAAGDGAGRTETRP
                     VSGGLLAQSIDRVDAVVRDESGAATGGDDPAYWTLACGEPADEATLADLAFAWRAVRA
                     VKSNAILLARDGASVGIGMGQVYRVDSCHLAVSRAGEERARGAVAASDAFFPFADGPQ
                     VLLDAGVAAIVQPGGSMRDAETIAAATAAGVTMYLTGTRHFAH"
     gene            complement(4178..4780)
                     /gene="purN"
                     /locus_tag="PLK64_08740"
     CDS             complement(4178..4780)
                     /gene="purN"
                     /locus_tag="PLK64_08740"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPV79874.1"
                     /translation="MSLTPIVVLVSGTGSLLQALIDAGTDLGYGVRVAAVGADRPGTL
                     GQARAEAAGIPTFVCRVADFPSRADWDAALAQNIAAHAPAFVVSAGFMKLLGPVTLGQ
                     HTVVNTHPALLPSFPGAHAVRDALAYGVRVTGTTCHLVDAGVDTGPIIEQHAVAVEPD
                     DTEDSLHERIKVLERLMLVDVVGRLARQGFTVTGRKVTIP"
     gene            complement(4813..6006)
                     /gene="lhgO"
                     /locus_tag="PLK64_08745"
     CDS             complement(4813..6006)
                     /gene="lhgO"
                     /locus_tag="PLK64_08745"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019158526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HPV79875.1"
                     /translation="MSTIAVVGGGIVGLATARVLARRGEQVVLLEKESALAQHQTGRN
                     SGVVHAGLYYPPGSLKARMAVAGAQSMYAYARAKGIAYDNCGKLVVACDESELPGLDR
                     LAERAEANGVPARLLDPAAAREIEPYVRCVRALHVETTGIIDYPAVCRALADDIGAQG
                     GEIRLGVGVASAESTPHGVEVHTTTGETLRADALVACAGLYADRVALACGLEPDARIV
                     PFRGEYFELAPEKNYLVRNLIYPVPDPRFPFLGVHLTRMVHGGIHAGPNAVLALRREG
                     YTWRDVDVAELRESLAWPGLWALAKRNIVPGAREVVRSLSKRQFAASLSRLVPGIEAA
                     DLRPAAAGVRAQALRRDGSMVEDFLVQTQGRQVHVLNAPSPAATAALEIADHLADSVE
                     QVLAS"
     gene            complement(6016..6942)
                     /locus_tag="PLK64_08750"
     CDS             complement(6016..6942)
                     /locus_tag="PLK64_08750"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HPV79876.1"
                     /translation="MLLLLVLIAAAYAVVRNWRDVSATVTHLSWRAWIPAFLILPVAI
                     GCSTMSWQVLVDELGEPIGARRGAQIFLVGQLGKYVPGSVWAYVLQLELGRRAGVARA
                     RIFAATVFSLAVVVVAALLAGGLAVPALGEANPDLSSLRWLYLLLPVALVCLHPRILT
                     WAVRQGFRMLGRPAPDHPIRKRAVLTSLAWALASYLAFGTHLWLLVRASTPISVSALP
                     LTIGTMAIAMISGLFVFLLPSGAGVREAILVAGLGPFVGTGPAIALAAVSRVLLTIAD
                     LVTAGASAGLATMEQRRHGPYHGDPGIDADEA"
     gene            7095..8054
                     /locus_tag="PLK64_08755"
     CDS             7095..8054
                     /locus_tag="PLK64_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPV79877.1"
                     /translation="MSAPLAIALVGSGSMGLNHARVIATGPQTTLAVVIDPSESVGRA
                     VAERHGARWAPSLDALSGVDAAVVAASTEHHRALALPIILAGLPLLIEKPVCPSLAQT
                     REVVDASRAAGTPLQCGLLERFNPAVVTALRMVEAPCYVRAERHSPYASRIRTGVGWD
                     LLVHDVDLVVRAFGGQAPHSVGVQVGRFHPESATGAEDVVESTLQFQGGGIASVSASR
                     IGQRKVRSMVIQELGRMIEVDLLRRGVTVYRHTTIEGEAAHGGYRQMTEMEVPEILGV
                     EPLVAQLAHFVDLIEGRVDADTERESILPAHEVVDTVLAAGGR"
     gene            complement(8089..8751)
                     /locus_tag="PLK64_08760"
     CDS             complement(8089..8751)
                     /locus_tag="PLK64_08760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPV79878.1"
                     /translation="MAARIAEVTHGGVAATVIPFGVDPAIFWPGPERADGRPVRFGIV
                     KTLEPHYRIDVVIDAFARYVSDRDPDAATLDIYGSGALLPELQARIARLGVSDRVVVH
                     GGIAHEAVPAALRALDVFVLASETESFGVAAVEAMACGVPVIASDAPGFVEVLDSGRY
                     GLLYPRGDVGALVQHMSGLGADVEARRRLRVAGMEGARRYDWRRNVVDMFGLLTSMAS
                     RA"
BASE COUNT         1423 a         3129 c         2990 g         1211 t
ORIGIN      
        1 caagacctca tgtccgatcc gcagcgccga tccgagctgg gccgcaatgc ccggacgtgg
       61 gccaatctga accttcgcgt tgatcggttc gtgcagcaag ttctcgggct cgcgcgctga
      121 ccggccctcg tgccgacgga gcgccgcgcg ggacagcgaa tcccgcggcc cgtcgagcca
      181 gacgagtcag aggcgaacga gggcgaaggc gaccgccaca gtggcaccca gaccgagcca
      241 gcagacgacg tcgaccgagc gccgacgaac tgccagccca ccggcccgcc ccagagggcg
      301 gatcgcccgc aacagccccc cgacagcaca ggccgtggcc agaaggtaac cccccggccg
      361 gacctggccg agccccacca cgagcagccc ggcaccggcg atgacgccga cgacccacca
      421 cagtcccaag cgccgaacgg cgcgcagagc cgagcgggtt tcggtggtca tgcgctggcg
      481 gcttggcgtt ccgcggcgag cacgacgttg atgagcaaca gggcccgagt catcggcccg
      541 acgccaccgg ggttggggct gacccagccg gctacctcgg cgacgtccgc ggccacatcg
      601 ccggccaccc gcgacttgcc cgtcgcggga tcagtcaccc ggctcacgcc gacgtcgagg
      661 accgcagcgc caggcttgac catgtcggcg gtgatgatgt tgggcacccc ggccgccgcg
      721 acgacgatgt cagcttgacg cacctcggcg gccaggtcgc gagtcccggt atggcacaac
      781 gtgactgttg cattctcgct cttgcgggta aggatcagcc ccagggggcg cccgaccgtc
      841 aggccacgac cgacgacgca gaccttcgcg cccgcgatcg gcactccgaa gcgacgcagc
      901 aactcaatga cccccatggg cgtgcacggc agcggtgccg gacggcccag gacgagccag
      961 cccaggttca tcgggtgcag gccgtcaacg tccttggccg ggtcgacccg cgagagcagc
     1021 gccatctcgt ccaacccggt cggctgctgg acgaggaagc ctgtgcaccc cgggtcggcg
     1081 ttgagctcgt cgatgacggc ctcgacctcg gcctgggtcg ccgtcgccgg caaatcccgg
     1141 cggatgctgc ggatgcccaa ctcggcgcag tcctggtgtt tggccccgac ataccactga
     1201 ctgcccgggt cgtcaccgac aagaacggtg ccgaggcccg gaacgattcc gcgctcggcg
     1261 agcgccgcca cccgtgccgc caactcggcc ttgaccgtgg tgagcgtggc tttgccgtcg
     1321 aggatctgcg ccgtcatgcg ccaatccttc catgtcggcg caccccaccc cgtagcgtgg
     1381 atggttcacc gcccgccacc ctccgaggca gcatgtccca ccgcagaccg actggtatgc
     1441 cgcccgctca cggcttgctc gtccgcgctg cgctcggcgc cttccccctc acacccgcag
     1501 tcgcactcgc actcatcctg acagcgtgct cgacagcgcc ggacggttca ggccaacagc
     1561 cgtctggtcc caccggcgcg gcgtctcggg gcgaattctc cgtgcctgtt ctgacggtcg
     1621 gtacgggcca gcccacagcg accgcgacgc tcactcccac agccctgccg ccgccgacgc
     1681 ccacaccccc acccataccc ccaccgaccc ccacgctgac gctgcccggc ggcggcacga
     1741 cggtcttccc tgatcgccgc ctcgtggggt tctcgggtta tccgggggcg cctgccctgg
     1801 gccgcctcgg ggtcggcaac ctcgacgagc ggggcgccga catcaccgcc ctcgccggcg
     1861 agtatgccgc cgggcgccgc ccactccccg ttttcgaact catcgccact gtggtccaca
     1921 gccggcccgg ggccgacggg ctctaccgga cccggatcga cgagggcgtc gttcggcgtc
     1981 atctcgaggc ggcccgacgg cacggcgcca tcctcctgct caacatccag cccggccggg
     2041 cggccttcct cgacgaggtc aagcactggg agccctggct gcgcgagccg gacgtgggcc
     2101 tcgcgctcga cccggagtgg gcggtcacgg ccgggcaggt ccccgggaag gtgttcggcc
     2161 ggacgaccgg cgcggagctc gatgcgatcg cggcatacgt cgcggggatc gtcgccgcaa
     2221 accgactgcc ggagaaggtc atggtctatc accaactcag ccggtcgatc gtccgcgagc
     2281 cctccggcct gcagccgcat cccggggtga ccctcgtcgt gtcggtcgac gggatcggga
     2341 gccgggcgca gaaggcggcg acgtggcgct cgatcgtcgc cgaaaccccc gatcacgtcc
     2401 acaaggggtt caaactcttc tacgacgaag acgcccagat cggaccgctc atgaccccgg
     2461 ctgaggtcct cgcgctcacg ccccagccgg agtacgtcct ctacgagtga tcggtctcgt
     2521 cgagatcagt gggcgaagtg gcgggtcccg gtgaggtaca tcgtcacccc ggcggccgtc
     2581 gcagccgcga tggtttcggc gtcgcgcatc gacccacccg gctggacgat cgccgcgacc
     2641 cccgcatcga gcaggacctg cggcccgtcg gcgaagggga agaacgcgtc cgacgcggcc
     2701 accgcgccgc gcgcccgctc ctcccccgcc cgcgagaccg cgaggtggca cgagtcgact
     2761 cggtagacct ggcccatccc gatgcccacc gaggcgccgt cgcgggccag caggatcgcg
     2821 ttggacttca cggcacgcac cgcgcgccag gcgaaggcga ggtcggccaa agtcgcctcg
     2881 tccgcgggct caccacaggc gagcgtccag tatgccgggt cgtcaccacc cgtcgcggcc
     2941 cccgactcgt cgcgcaccac ggcgtccacc cggtcaatgc tctgagcgag caatccgcct
     3001 gataccggcc gggtttcggt gcgcccagcc ccgtcaccgg ccgccgctgc atcgagcgca
     3061 gcgaagtcga cggtgagcag tcggatgttc ttcttggccg tcagcacggc cagtgcgtcg
     3121 tcatcgaaac tcggcgccac gaccacctcg gtgaagatgt ccttgaccgt ctcggccatc
     3181 tccagcgtca ccggccggtt gacggcgata acgccgccga aggccgacac cgggtcgcag
     3241 gcgtgcgccc ggcggtgcgc ctgggcgatc ccgccttcct ccgtccccac ggcgatccca
     3301 cacgggttgg cgtgcttgat gatcgcgcag gtcggcaggc cgccatggtc acggcaggcc
     3361 cgcagcgccg cgtccgcgtc gacgtagttg ttgtaggaca tctccttgcc gtgcagctgg
     3421 gtcgccgagg cgatgccggg cgcgccactg agatagagcg ccgcctgctg gtgcgggttc
     3481 tcgccgtagc gcagccccgc ggcccgcgtg aacgtcgccc ccgcgaaggc cgcgaacccg
     3541 gtcccgtcgg aggtgtccgc gtaggcgcta ctgcccatcc acgaggccac agcgacgtcg
     3601 tatgccgcag tgtgggcaaa cgcctcggcc gccagccgct ggcgggccgc gaaggtgaac
     3661 ccgccggagc ggacggcagc cagcgcggcg gcatactgcg cggggctggt gagcaccgcg
     3721 acgctcgggt ggttcttcgc ggcggcccgg accatcgacg gcccaccgat gtcgatctgc
     3781 tcgacgcact cgtcgggggt cgccccggaa gcgacggtcg cggcgaaggg gtagaggttg
     3841 acggcgacca actcgaacgc ctcgacaccg agctcggcga gctgggccag gtggtcgggc
     3901 ttgcgggtgt cggcgagcag cccggcatgc acccgcgggt gcagcgtctt gacccgcccc
     3961 tccaggcact cggggaagcc ggtgaggtcc tcgaccttgg tcaccggcag cccgagcccg
     4021 gcgatgagcg cggccgagcc gcccgtcgag acgagctcga cccctgcgtc ggcaagcccg
     4081 cggacgaggt cctcgagccc gctcttgtcg tagacggaca ccagggcccg gcgcacgggg
     4141 cggacgtcct gggtcgggcc gggtgcgctg acgctgctca cgggatggtg accttcctgc
     4201 cggtaacggt gaaaccttga cgggccagcc ggcccacgac atcgacgagc attaggcgtt
     4261 cgagcacctt gatccgctcg tggagggagt cttcggtgtc gtcgggctcg acggcgaccg
     4321 cgtgctgctc gatgatcggc ccggtgtcga cgcccgcatc gaccaggtgg caggtcgtgc
     4381 cagtgaccct gacgccatag gccagcgcgt cgcgcaccgc atgcgcgcca gggaaggacg
     4441 gcagcagcgc ggggtgggtg ttgacgaccg tgtgctgccc gagggtcact gggccgagca
     4501 acttcataaa gcctgccgag acgacgaagg cgggcgcatg ggccgcgatg ttctgcgcga
     4561 gagcggcgtc ccagtccgcg cgcgacggga agtccgcgac gcggcaaaca aaggtgggta
     4621 tgccggccgc ctccgcccgc gcctgcccga gagttccggg gcgatcggct ccgacagccg
     4681 cgacccggac gccatagccg agatcggtgc cggcgtcgat gagcgcctgc agcaacgacc
     4741 cagtccccga gacgaggacg acgatcggag tgaggctcac gtggagcaac cctatccggc
     4801 tcagggcgga ggtcaggaag cgagcacctg ctcgacgctg tcggcgaggt ggtcggcgat
     4861 ctccagggca gcggtggcag cgggagatgg agcgttgagg acgtggacct ggcgaccctg
     4921 ggtctggacg aggaagtctt cgaccatcga gccgtctcgc cgaagcgcct gggcccgcac
     4981 acctgcggcg gcgggacgca gatcggcggc ctcgatcccc gggacgagcc gggacaggct
     5041 ggcggcgaac tgtcgcttgg acaaggaacg aacgacctcc cgagcgccgg gaacgatgtt
     5101 gcgtttggcc aaggcccaga gccccggcca ggccaacgac tcgcgcaact cggcgacatc
     5161 cacgtcgcgc cacgtgtagc cctcacgacg cagcgccagg acggcattgg gcccggcgtg
     5221 gatgccgccg tgcaccatgc gcgtgaggtg caccccgagg aagggaaacc gcggatccgg
     5281 gaccgggtag atgaggttgc gaacgaggta gttcttctcg ggtgccagtt cgaagtactc
     5341 cccccggaac gggacgatcc gcgcgtccgg ctccagtccg cacgcgaggg cgacccggtc
     5401 ggcatacagg cccgcgcagg cgaccaaggc gtcagcccgg agcgtctcgc cggtcgttgt
     5461 gtggacctcg accccgtggg gcgtcgactc ggcagaggcc acccccaccc cgagcctgat
     5521 ctcgccaccc tgagcgccga tgtcgtcggc gagcgcgcgg catacggcgg ggtagtcgat
     5581 gattccggtc gtctcgacgt gcagcgcccg gacgcatcga acgtagggct cgatctcgcg
     5641 agcagcggcc gggtcgagca gccgtgcggg gacgccgttg gcctcggcgc gctctgcaag
     5701 gcgatccagc cccggcaatt ccgactcgtc gcaggcgacg accaacttgc cgcagttgtc
     5761 gtaagcgatg ccttttgctc gggcataggc atacatcgac tgggcaccgg ccacggccat
     5821 ccgggccttg aggctgcccg gggggtagta gaggcccgcg tggacgactc cggaattgcg
     5881 cccggtctgg tgttgggcga gcgccgattc cttctccagc agcacgacct gctcgccccg
     5941 gcgggccagc acgcgcgccg tcgccagacc gacgattccc ccaccgacta cggcaatggt
     6001 gctcacacgc cgagcttatg cctcgtccgc gtcgatcccg gggtcgccgt ggtaaggccc
     6061 atgacgacgc tgctccatgg tcgccaggcc tgcggaggca cctgctgtca cgaggtcggc
     6121 gatggtgagc agcactcggg acactgccgc gagtgcgatg gccggcccgg tgccgacgaa
     6181 tgggccgagc ccggcgacga ggatggcctc gcgcacgccg gctcccgagg gcaggaggaa
     6241 gacgaacagt cccgaaatca tcgcgatcgc catcgtgccg atggtcagcg ggagcgccga
     6301 cacgctgatc ggcgtgctgg cccgcacgag caaccacagg tgcgtgccga aggcgaggta
     6361 ggacgccagt gcccaggcca gcgaggtgag cacggcgcgc ttgcggatcg ggtggtcagg
     6421 cgccgggcgg ccaagcatcc ggaacccctg ccgcacggcc caggtaagga tccgggggtg
     6481 aaggcagacc agagccacgg gcagcagaag gtagagccag cgcagactgc tcagatcggg
     6541 gttggcttca ccgagggctg gcaccgccag accaccggcc agcaacgccg cgacgacgac
     6601 gaccgccagc gagaacacgg tcgcggcgaa gatccgtgct cgcgccaccc cggcccgccg
     6661 accgagctca agctgaagca cataggccca caccgatccg gggacgtact tgccaagctg
     6721 ccccacgagg aagatctggg caccgcggcg agcgccgatg ggctccccca gctcgtcgac
     6781 cagcacctgc cagctcatcg tcgaacagcc gatcgcgacc ggcaggatga ggaaggccgg
     6841 gatccacgct cgccacgaca ggtgggtaac cgtggcgctc acgtcacgcc agttgcgcac
     6901 gaccgcgtat gccgcggcga tgagcaccag aaggagcagc acggctcgcg cgatacgcaa
     6961 cgatcgggct cgggtcacag caagccacgc gtcgggcggg tatggggcag gagcgccggg
     7021 gcgggctcaa tcacgggact agtctggccc acgcacccgc ccgggaccgc catcggtgcc
     7081 cagaggagga agttatgtct gcgccactcg ccattgctct cgttggatcg ggatcgatgg
     7141 ggctcaacca cgctcgggtc atcgcgacgg ggcctcaaac cacccttgca gtcgtcatcg
     7201 acccgtccga gagcgtcggg cgtgcagtcg ccgaacgcca cggcgcccgg tgggcgccca
     7261 gcctcgatgc tctgtcgggg gtcgacgccg ccgtcgtcgc cgcgtcaacg gagcaccacc
     7321 gtgccttggc cctgcccatc attctcgcgg ggcttccgct gctgatcgag aagccggtat
     7381 gcccgtcgct tgcccagacg cgagaagtcg tcgatgcgtc ccgggccgcg ggcacccctt
     7441 tgcagtgtgg gctacttgaa cgcttcaacc cggccgtcgt taccgccctg cggatggtcg
     7501 aagccccgtg ctatgtccgc gcggaacggc actctcctta tgcctcgcgg atcaggacgg
     7561 gcgtcggctg ggacctcctc gttcacgacg tcgacctggt cgtgcgagcc ttcggcggcc
     7621 aagcccctca ctccgtcggt gtgcaggtgg gccggttcca ccccgagtca gcaactggcg
     7681 ccgaggatgt cgtcgagtcg acattgcagt ttcagggagg ggggatcgca tcggtttcgg
     7741 ccagccgcat cgggcagcgc aaggtccgct cgatggtgat ccaggagctg ggccggatga
     7801 tcgaggtcga cctgctccgg cgcggagtca ccgtctatcg gcatacgacg atcgagggag
     7861 aggccgcaca cggtggctac cgacagatga ccgagatgga ggtcccggag atactcgggg
     7921 tggagccatt ggtcgctcag ctcgcccact ttgtcgacct catcgaggga cgggtcgacg
     7981 cggacaccga acgtgagtcg atcctgccgg cgcatgaggt cgtcgatact gtcctggccg
     8041 ccggcggccg ctagcttcca ggcggtcttg agatcgccgt cggatcggct aagcgcggct
     8101 cgccatcgac gtgagcagac caaacatgtc gaccacattg cgccgccagt cgtagcgccg
     8161 agcgccctcc atcccggcca cccgcagtcg gcgccgcgcc tcgacatcgg cgccaagacc
     8221 actcatgtgt tggaccaagg caccgacatc gccgcgtgga tagagcagcc catagcggcc
     8281 gctgtcaagc acttcaacga acccgggcgc atcggatgcg atgacgggga cgccgcacgc
     8341 catcgcctcg actgcggcga ccccaaagct ctcggtttcg ctggccagga cgaaaacgtc
     8401 caaagcgcgc aacgcagctg gcactgcctc gtgggcgatc cctccgtgca ccaccacgcg
     8461 gtcggatacc ccgagccgcg ctattcgcgc ctgcaactcg ggcaggagag caccgctgcc
     8521 gtagatgtcc aaggtggccg catcggggtc ccggtccgac acgtatcgag cgaatgcatc
     8581 gatgacaacg tcgatgcggt agtgcggctc aagagtcttc acaatgccaa accgcacggg
     8641 cctcccatcg gcacgttcag ggccgggcca gaagatggcc ggatccactc cgaagggaat
     8701 caccgtcgcc gccacgccgc cgtgggtgac ctccgcgatc cgggcggcca tca
//
LOCUS       DAOMZY010000132         8731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_7606_length_8731_cov_5.652605, whole genome shotgun sequence.
ACCESSION   DAOMZY010000132 DAOMZY010000000
VERSION     DAOMZY010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7606_length_8731_cov_5.652605"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="PLK64_08765"
     CDS             <1..778
                     /locus_tag="PLK64_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPV79879.1"
                     /translation="VAMLAGVSVDYYVRMERGSLAGVSEGVLASLADALRLDDAERDH
                     LEALARESGASGTRAASNRRTKAATPVVRPAIRQVLDAITDAPAWVRNGRHDILAMNP
                     LARALYAPVLADPRRPANTTRFIYLQPEAARELFVDYDQVAKDGAAMLRLEAGRNPHD
                     KDLIQLVGELSTRSELFRQRWASQDVRYHRSGRKRLRHPVVGQLDLDYEAMELLSDPG
                     LVLNMYTAAAGTPTSDALKILASWVAGQEQLATELAPSNG"
     gene            813..1661
                     /locus_tag="PLK64_08770"
     CDS             813..1661
                     /locus_tag="PLK64_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018761972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HPV79880.1"
                     /translation="MTDTETPSPRRRAVFLDVDGTYAVFGAAPAGHVAAVRAARDAGH
                     LVFLCTGRPMSMVPAHLLAAGFDGVVAAGGAYAVLGDEVLLDIRFPADLAARTVEVMD
                     SYGVTYLLEAPEAVYTASGTEVQLRRAYRAHLADAPSATTVVSDILASVRPVPAAGAV
                     SFAKVSVVVSSVPVEKLGAEVGEQLAVLPSSVPALGEEAGEIFLAGVHKAVGMEAIVR
                     RLGLARGDVIGFGDGLNDLEMLEYAGVGVAIEGSHERLLAVADRTAAGPEVEGLVQAF
                     AELGLR"
     gene            2251..4131
                     /locus_tag="PLK64_08775"
     CDS             2251..4131
                     /locus_tag="PLK64_08775"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HPV79881.1"
                     /translation="MASPTKYDLLAAEVIRGVGGADNVASVTHCATRLRFALKDRDKA
                     DKAAVEATRGVITVVESGGQFQVVIGNTVNNVYEAIVAKGDVKSGGEVAAGGFLAKAI
                     DLVTSIFTPFLWVLAGTGLIKAFLALAGKLSPDFATSSTYAILFAAGDALFQFLPMLL
                     AVTAAKKFKANVYTSLAIAAALIYSATIPVIADAKGVSMTLGAFDAASNLTFLGIPVV
                     MVSYLSAVVPTILAIYAQAKLEHWLERVIPETVRNFVVPLVAVAVVVPLTFLAIGPVA
                     DLIGKGVSGGVNALWSLSPVIGGAVMGALWQVFVIFGVHWGFVPVMIQDLTTQGYSLL
                     TGPLFAAVLAQGAAATAVMIKTRNKELKEIAGPAALSAVLAGITEPAIYGVTLRLKKP
                     FIYGCIGGAIGGGIAAAGGSAAKGFVIPGLITMSAAVDVGNFGLQVAGTVIAMAIAFG
                     LTMVLGFQDIPAKAALDDAAATGEVASEAVSVAAPAIGEVVSLESVPDKVFASGALGA
                     GLAVVPAVGEAFAPVSGTLITATSHAYGIKTDNGLEVLVHIGIDTVKLGGKHFSPKVV
                     QGQRVTAGQTLAEFDIAAIEKAGYNPVVVMIVTNNGPFSAAVPVASGSIRMGETALDL
                     VG"
     gene            4261..5688
                     /locus_tag="PLK64_08780"
     CDS             4261..5688
                     /locus_tag="PLK64_08780"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HPV79882.1"
                     /translation="MTSTPQTYFPDGFLWGGATAANQIEGAYTEAGKGLSIQDVMPKG
                     IFGAFTEAPTADNLKLEGIDFYHRYAEDIALFAEMGFSVFRFSIAWSRIFPLGDETEP
                     NEEGLAFYDRVLDELERHGIQPLVTISHYETPLHLARTYGGWVNRDLIGFYERYVRVL
                     FNRYGHRVKYWLTFNEINSVIHAPFMSGGIPTPLEELTKSDLYQAVHHELVASALATK
                     IGHETNPRIQVGCMVLAMPIYPLTSSPDDAIAVMNADHGNLMFSDVHTRGVYPGYALR
                     MFREHGIELDITDEDRELLTNTVDFVSFSYYMSICETADPAQKTAGQGNIMGGVPNPH
                     LPASQWGWQIDPQGLRVVLNQFWDRWQKPLFIVENGLGARDELVEVDGIPTVVDDARI
                     DYLNDHLVQVGEAIADGVNLLGYTTWGPIDLVSASTAQLSKRYGFIYVDRNDDGSGTL
                     ARYRKKSFAWYAEVIRTNGASLVRG"
     gene            5725..6591
                     /locus_tag="PLK64_08785"
     CDS             5725..6591
                     /locus_tag="PLK64_08785"
                     /inference="COORDINATES: protein motif:HMM:NF013070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HPV79883.1"
                     /translation="MQVVKVFNNNVVLGADGHGGEFVLLGRGLGFRVSPGMDIDATRV
                     ERTFVPSGVTTADRLAALVGEMPLADIELTERIVAMARSSLGSYVTDLVVVPMADHIG
                     FALRRLAEGAPEIEYPLRWEVHYLYPAEVAVARQALDLIELERGVRLPDIEAVPLALH
                     FVNAQFGAGDINATLQMTELLQRILAIIGEEYNVVIDEGAVAVARFVTHLRYLFHREQ
                     QGKKLQQTPAELHEALRAARPREYASAGRIGELLRARFGWEITNDEILYLALHVSRLI
                     DDGSTPVPAEAT"
     gene            6588..6827
                     /locus_tag="PLK64_08790"
     CDS             6588..6827
                     /locus_tag="PLK64_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose/sucrose transporter subunit IIB"
                     /protein_id="HPV79884.1"
                     /translation="MTTAEQILTAVGGPDNVASLTRCWARLRFELHDAAVVDEAAIGA
                     LPEVALVVTQHGQFQVAMRTGLLEVFDELVALLER"
     gene            6925..7194
                     /locus_tag="PLK64_08795"
     CDS             6925..7194
                     /locus_tag="PLK64_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPV79885.1"
                     /translation="MATRSVIIASSVGLHARPASIFVQAANATGLDVEIGRPGEEPVD
                     ATSILGVMSLGAGHGDEVVLTAEGDGADAALDELVALLSRDLDAE"
     gene            complement(7285..7668)
                     /locus_tag="PLK64_08800"
     CDS             complement(7285..7668)
                     /locus_tag="PLK64_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HPV79886.1"
                     /translation="MIEYLALEDVLTLIDDLGVGPVRDVGLLDSAVHRPQASVFGVDA
                     YPNIDEKAAALLESLVRNHTLIDGNKRLGWLAIVVFYGLNDITLDAPDDEAYDLVIAM
                     ASGASTYQEAAAHLSRWRVGEHPVP"
     gene            complement(7665..7868)
                     /locus_tag="PLK64_08805"
     CDS             complement(7665..7868)
                     /locus_tag="PLK64_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017823575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HPV79887.1"
                     /translation="MAMTLRLTPEDEEALTMLAQADGVSKQEATVRAIHEAASRRARG
                     DKVAGLSASARHRYADLLDRLGQ"
     gene            7986..8657
                     /locus_tag="PLK64_08810"
     CDS             7986..8657
                     /locus_tag="PLK64_08810"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPV79888.1"
                     /translation="MRWAVGPTSSKKSGAADRVAAVLREQIVKGLFPPGLRLSEEALG
                     SDLGVARNTLREAFRMLSQERLVVHRLNRGVFVRELTVAEIEDLYRLRSAIEIAALSQ
                     GGLVEPELGPLRAALAEADEAALREDWKAVGTAGLVFHSRLVQLAGSERLSAVMDGLM
                     AELRLAFHMAANVNELHARYALRNKMLFAMLEEGDITGAAVALRDYLTDSEADILGAY
                     SGVRA"
BASE COUNT         1331 a         2942 c         2926 g         1532 t
ORIGIN      
        1 ggtcgcgatg ctggcgggcg tgtcggtcga ctattacgtc cggatggagc gcggcagtct
       61 cgccggggtg tccgagggcg tcctcgcgtc gctcgccgac gcgctgcggc tcgatgacgc
      121 cgagcgcgac caccttgagg cgctcgcccg cgagtccggg gcctcgggca cgcgcgccgc
      181 gagcaaccgt cgcacgaagg cggcgactcc ggtcgtgcgg ccggccatcc gccaggttct
      241 tgacgcgatc accgacgccc cggcgtgggt ccgaaacggc cgtcacgaca tcctcgcgat
      301 gaacccgctg gctcgcgcgc tttatgcccc ggtgctcgcc gatccgcgtc gcccggccaa
      361 cacgacccgg ttcatctacc tgcagccgga ggcggcgcgc gagctgttcg tcgactacga
      421 ccaggtcgcc aaggatggtg ctgcgatgct ccggctggag gccgggcgca acccgcacga
      481 caaggacctc atccagctgg tcggcgaatt gtccaccagg agtgagcttt tccggcaacg
      541 gtgggcttcg caggacgtgc gttaccaccg gtccgggcgt aagcggctcc ggcaccccgt
      601 ggtcggtcag ctcgacctgg actacgaggc gatggagttg ttgagcgatc ctgggctggt
      661 cctcaacatg tacaccgcgg cggccggcac gccgacatcg gacgccctca agatcctcgc
      721 gtcgtgggtc gccggtcagg agcaactcgc caccgagctg gcgccgagca acgggtgagc
      781 cgacgcccga acctcggcta aggtcgaccg acgtgacgga caccgagacc cccagcccac
      841 ggcgccgtgc cgtcttcctc gacgtcgacg ggacgtatgc cgtcttcggc gccgcgcccg
      901 ctggacacgt ggctgcggtg cgcgccgccc gggacgccgg tcaccttgtc ttcctctgca
      961 ccgggcgccc gatgtcgatg gtgcccgcgc acttgctggc cgccgggttc gacggggtgg
     1021 tcgccgctgg tggtgcgtat gccgtgctcg gcgacgaggt cctgctcgac atcaggttcc
     1081 ccgctgacct cgccgcgcgg accgtcgaag tcatggacag ttacggcgtg acctatctgt
     1141 tggaggcccc cgaggcggtc tacacggcgt ccggcaccga ggtgcagttg cgccgggcct
     1201 accgtgccca cctcgccgat gcgccgtccg ccacgaccgt cgtgtccgac atcctcgcgt
     1261 cggtgcgacc ggtgccggcc gccggcgcgg tgtcgttcgc caaggtctcc gtcgtcgtgt
     1321 cgtccgtccc ggtcgagaag ttgggcgccg aggtcggcga acaactcgcg gtgctcccca
     1381 gctcggtccc ggccctcggc gaggaggcgg gtgagatctt cctcgccggt gtccacaagg
     1441 cggtcgggat ggaggccatc gtgcggcgcc tggggctcgc gcgcggggac gtcatcggct
     1501 tcggcgacgg gctcaacgac ctcgagatgc tcgagtatgc cggtgtcggc gtcgcgatcg
     1561 agggttcgca cgagcgcctg ctcgcggtcg ccgaccgcac cgccgccggc cccgaggtgg
     1621 aaggcctggt ccaggcgttc gctgagctcg gcctgcgctg acctgctgcg cgtccggatg
     1681 gcggcgggat cctttacagc cgtcggtcgg ggcgctaccg ttgccagcgg acgccctgcg
     1741 gtgtctacgc agttgggctt gtggaagttt gggattgtta ctgcgcggca ggcaaaaccc
     1801 aagtcggacc agcagcgact ctgatgggtc gcgtgcagtc gtggcttggg tttttttgtt
     1861 ccgtgaacat gcctgcggtg atgtgagtgg gccccggcct gggaagcggt ggccgggccc
     1921 tgctcgtcgg acgacgcgcg ctgtgcgctg cgcgtcgatc gacccaggga tatgccgcgt
     1981 gagctgtgag gggcttccgg ctcacgcggc atacccttgt ctgggagcct gaatcgagat
     2041 cctttacagc agtgggcggt tgccgctacc tttggtggcg gacgccgtgt ggcgtcccgg
     2101 aagtctggga ttgttactgc gcggcaggca aaacccaagt cgggccagag gtcgactctc
     2161 acgagtcgaa ggcggtcagg acttgggttt ttttgtgccc gaggcatgcc ccacgtaccc
     2221 gttcaggggt tggattcgag gaggaatcac gtggcaagtc ccactaagta cgacctgctc
     2281 gcggcggagg tcatccgcgg ggtcggcggc gccgacaacg tcgcatcagt aacgcactgc
     2341 gcaacccgac tgcggttcgc gctcaaggac cgcgacaagg ccgacaaggc cgctgtcgaa
     2401 gcgacgcgcg gtgtcatcac cgtcgtcgag tcgggcggtc agttccaggt cgtcatcggc
     2461 aacacggtca acaacgtcta cgaggcgatt gtcgccaagg gcgacgtcaa gtccggaggt
     2521 gaggtggccg cgggaggctt cctggccaag gcgatcgacc ttgtcaccag catcttcacc
     2581 ccgttcctgt gggtgctcgc gggcaccggc ctcatcaagg ccttccttgc cctcgccggc
     2641 aagctctccc cggacttcgc gacgtcttcg acctacgcga tcctcttcgc cgcgggcgac
     2701 gcgctcttcc agttcctgcc gatgctgctg gcggtgacgg ccgccaagaa gttcaaggcg
     2761 aacgtctaca cctcgctggc catcgcggct gcgctcatct actccgcgac catcccggtg
     2821 atcgcggacg ccaagggcgt gtcgatgacc ctcggcgcct tcgacgccgc cagtaacctc
     2881 accttcctcg gtatcccggt cgtcatggtg agctacctgt ctgccgtcgt cccgacgatc
     2941 ctggccatct acgcccaggc caagcttgag cactggctgg agcgcgtcat ccccgagacg
     3001 gtccgcaact tcgtcgttcc gctggtcgcc gtcgccgttg ttgttccgct gaccttcctg
     3061 gctatcggcc cggtcgccga cctcatcggc aagggtgtgt ccggcggcgt caacgcgctg
     3121 tggagcctca gccccgtcat cggtggcgca gtcatgggcg ctctgtggca ggttttcgtc
     3181 atcttcggcg tgcactgggg cttcgtcccc gtcatgatcc aggacctcac gacgcagggt
     3241 tactcgctgc tgaccggtcc gctcttcgcg gccgtcctgg cccagggtgc cgccgcgacc
     3301 gccgtcatga tcaagacccg caacaaggag ctcaaggaga ttgccggccc ggccgccctc
     3361 tccgcggtcc tcgccggcat caccgagccc gccatctacg gtgtcaccct gcgtctgaag
     3421 aagccgttca tctacggctg catcggtggc gccatcggtg gcggcatcgc tgctgcgggt
     3481 ggctcggccg cgaagggctt cgtcatcccc ggcctcatca cgatgagcgc ggccgtcgac
     3541 gtgggcaact tcgggctgca ggtcgccggc acggtcatcg ccatggccat cgccttcggc
     3601 ttgaccatgg tgctgggctt ccaggacatc ccggccaagg ccgcccttga cgatgccgct
     3661 gccaccggcg aggtggcctc cgaggccgtc tcggttgctg ccccggccat cggtgaggtc
     3721 gtctccctcg agtcggtccc ggacaaggtc ttcgcctcgg gtgcgctcgg tgccggcctg
     3781 gcggttgttc cggccgtcgg cgaggccttc gcgccggtgt cgggcacgct catcaccgcg
     3841 acctcgcacg cctacggcat caagaccgac aacggcctgg aggtgctcgt ccacatcggc
     3901 atcgacacgg tgaagctggg cggaaagcac ttctcgccca aggttgttca gggccagcgg
     3961 gtcactgccg gacagacgct cgccgagttc gacatcgccg cgatcgagaa ggccggctac
     4021 aacccggtcg tcgtcatgat cgtgacgaac aacggaccgt tctcggccgc ggtgcccgtc
     4081 gcctccggct ccatccggat gggcgagacc gcgctcgatc tcgtcggctg acccggaccg
     4141 gccggggcgg catacctgtt gccccggccg gaccacccgc gggtcccgcg tctgacccgc
     4201 cccttgatgc tgcccctctt acgggaggga agcccccact tcacgaagga ttttgtcccg
     4261 atgacgagca cccctcagac ctacttcccc gacggcttcc tctggggcgg cgcgactgcc
     4321 gcgaaccaga tcgaaggcgc ctacaccgag gccggcaagg ggctgtccat ccaggacgtc
     4381 atgcccaagg gcatcttcgg cgccttcacc gaggcaccga ccgcggacaa cctcaagctc
     4441 gagggcatcg acttctatca ccggtatgcc gaggacatcg cgctgttcgc ggagatgggc
     4501 ttctcggtgt tccggttctc gatcgcgtgg agccggatct tcccgctcgg cgacgagacc
     4561 gagcccaacg aggaggggct ggcgttctac gaccgggtcc tggacgagtt ggagcggcat
     4621 gggatccagc cgctcgtgac gatctcccac tacgaaacgc cgctgcacct cgcgcgcacc
     4681 tacggcggtt gggtcaaccg cgacctgatc ggcttctacg agcggtacgt gcgggtgttg
     4741 ttcaaccgct acggccaccg ggtgaagtac tggctgacct tcaacgagat caactcggtc
     4801 attcatgcgc cgttcatgtc cggcgggatc ccgacgccgc tggaggaact caccaagagc
     4861 gacctctacc aggccgtcca ccacgagctc gtcgccagcg cgctggccac caagatcggc
     4921 cacgagacca accctcggat ccaggtgggg tgcatggttc tggcgatgcc gatctacccc
     4981 ctgacgtcga gcccggacga cgccatcgcg gtcatgaacg ccgatcacgg caacctcatg
     5041 ttctccgacg tgcacacccg cggtgtctac ccggggtatg ccctgcggat gttccgcgag
     5101 cacggcatcg agttggacat cactgatgaa gaccgcgaat tgctcacgaa caccgtcgat
     5161 ttcgtgtcgt ttagctacta catgagcatc tgcgagaccg ccgacccggc ccagaagacc
     5221 gccggccagg gcaacatcat gggcggcgtc ccgaacccgc acctgccggc gtcccagtgg
     5281 ggctggcaga tcgaccccca gggtcttcgc gtggtgctca accagttctg ggaccggtgg
     5341 cagaagccgc tgttcatcgt cgagaacggc ctcggggcac gcgacgagct cgtcgaggtg
     5401 gacggcatac ccactgttgt tgacgacgcg cggatcgact acctcaacga ccacctcgta
     5461 caggtcggtg aggcgatcgc cgacggcgtc aacctcctgg gctacacgac gtgggggccg
     5521 atcgacctgg tgagcgcgag cacggctcag ctgtcgaagc gatacggctt catctatgtc
     5581 gaccggaacg acgacggaag tgggacgctg gcgcgatacc gcaagaagtc gttcgcgtgg
     5641 tacgccgagg tgatccgcac taacggggcg agtctggtcc gcggctgatt cctccggcac
     5701 tctggaggcg ggaaggagga gcagatgcag gtcgtcaaag ttttcaataa caacgtcgtg
     5761 ctcggggccg atggccatgg cggcgagttc gttctgctcg gccgcggtct cggcttcaga
     5821 gtgtcaccgg ggatggacat cgacgcgacc cgagtcgagc gcacgttcgt gccgagcggg
     5881 gtcaccaccg ccgaccggtt ggccgcgttg gtcggtgaga tgccgctggc cgacatcgag
     5941 ctgaccgagc ggatcgttgc gatggctcgg tcgtcgttgg gctcctacgt caccgatctc
     6001 gtcgtcgtcc cgatggccga ccacatcggc ttcgccctgc gtcggttggc cgagggggcg
     6061 cccgagatcg agtacccgct gcggtgggag gttcactacc tctacccggc ggaggtcgcg
     6121 gtggcccgcc aggcgctgga cctcatcgag ctcgaacgtg gcgtccggct gccggacatc
     6181 gaggcggtgc cgctggccct gcacttcgtc aacgcgcagt tcggggccgg cgacatcaac
     6241 gcgacgctgc agatgaccga gttgctgcag cggatcctcg cgatcatcgg ggaggagtac
     6301 aacgtcgtca tcgacgaggg ggccgtcgcg gtggcccggt tcgtcacgca cctgcgctac
     6361 ctcttccacc gggagcaaca gggcaagaag ctccagcaga cgccggcgga gttgcacgag
     6421 gcgttgcggg cggcccggcc tcgcgaatat gcctcggccg ggcggatcgg tgagctcctg
     6481 cgcgcccggt tcggttggga gatcaccaac gacgagatcc tctacctcgc gttgcacgtc
     6541 agccgtctga tcgacgacgg ctccacccct gtccccgcgg aggccacatg accacggctg
     6601 agcagatcct gaccgcggtc ggcggcccgg acaacgtcgc gagtctcacc cggtgttggg
     6661 cgaggctgcg tttcgagttg cacgacgctg ccgtggtcga cgaggccgcc atcggcgcgc
     6721 tgcccgaggt cgctctggtc gtcacacagc atggccagtt ccaggtcgcc atgcgcaccg
     6781 ggctgttgga ggtcttcgac gagttggtgg cgcttctgga gcgttgaccg acctgcgact
     6841 cgttgtccct gtccgcctcg actgctccct caatcgatcg tcttgtcacg tttcgaacgc
     6901 caatccacgt cccaaggaga acccatggcc acccgttccg tcatcatcgc gtccagcgtc
     6961 ggcctgcacg cccgccccgc ctccatcttc gtccaggcag ccaacgccac cggcctcgac
     7021 gtcgagatcg gccgtcccgg tgaggagccc gtcgacgcca cgagcatcct cggggtcatg
     7081 tccctgggtg ccggccacgg cgacgaggtc gtgcttaccg ccgagggcga cggcgccgac
     7141 gcagccctcg acgagttggt cgccttgctc tcgcgcgacc tcgacgccga gtaggcgctg
     7201 cgcaccaccc gcagaggtat gccgcccgct cacctgagcg ggcggcatac ctttgcgtcg
     7261 ttggcccctt cgcgaggaag cgtgttacgg caccgggtgc tcgccgactc gccaccggga
     7321 caggtgtgcg gcggcctcct ggtaggtcga ggcgccgctg gccatggcga tcaccaggtc
     7381 gtaggcctca tcgtcaggag cgtcgagggt gatgtcgttc aagccgtaga acacgacgat
     7441 ggcaagccag ccgagtcgct tgttcccgtc gatcaaggtg tggttgcgca ccagggactc
     7501 caacaaggcg gcggccttct cgtcgatgtt cgggtaggca tcgacaccga acacgctcgc
     7561 ctggggtcgg tgaacggctg agtcgagcag accgacatcg cggactggtc ccacgcccag
     7621 atcgtcgatc agcgtcagaa catcctcgag agcgaggtat tcaatcactg gccgaggcgg
     7681 tcgagcagat ccgcatatcg atgccgtgcc gaggccgaca gtccagccac cttgtcgccg
     7741 cgggcccgac gagaggctgc ctcgtggatc gcgcgcacgg tcgcctcctg cttgctcacg
     7801 ccgtcggcct gggccaacat ggtgagcgcc tcctcatcct cgggagtcaa ccgcagagtc
     7861 atggccatga ggtcatggta tcaagttggt atcgcaccca cggagtcaaa gcccgccgca
     7921 ggtatgccgc gtggcggggt gctcgcgcgg cgataggatc gcttcagttc gttgaacaaa
     7981 acaacgtgag gtgggcagtg gggccgacgt cgtcgaagaa gtccggtgct gccgaccggg
     8041 ttgcggcggt gctgcgggag cagatcgtca aaggcttgtt ccccccgggt ttgcggctgt
     8101 ccgaggaagc gctagggagc gatctggggg tggcgcgcaa caccctgcgt gaggcgttcc
     8161 ggatgctcag ccaggagcgc ctcgtcgttc accggctcaa ccggggcgtg ttcgtccgcg
     8221 agctcaccgt ggcggagatc gaagacctct accggttgcg ctcggctatc gagatcgcgg
     8281 cgttgtcgca ggggggcctc gtcgagcccg aactcgggcc attgcgtgcg gccctcgccg
     8341 aggccgatga ggctgccctc cgcgaggatt ggaaggcggt cgggacggct ggcctcgtct
     8401 tccattcgcg actggtccaa ctcgcaggca gcgagcggct gtctgcggtg atggacggac
     8461 tcatggccga gctgcgcctg gcctttcaca tggcggcgaa cgtcaacgag ttgcacgcgc
     8521 gctatgccct gcgcaacaag atgctcttcg cgatgctcga ggagggcgac atcacgggtg
     8581 cggcggtcgc gttgcgggac tacctgacgg attcggaggc cgacatcctc ggggcctact
     8641 ccggagtacg ggcctagtag tcagattgtt gaacaaaact tgacgcgacc gcgagcctct
     8701 gggcagactg gccacgcccc ggcttgaccc c
//
LOCUS       DAOMZY010000133         8719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_7622_length_8719_cov_6.503232, whole genome shotgun sequence.
ACCESSION   DAOMZY010000133 DAOMZY010000000
VERSION     DAOMZY010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 8719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8719
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7622_length_8719_cov_6.503232"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PLK64_08815"
     CDS             complement(<1..219)
                     /locus_tag="PLK64_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPV79889.1"
                     /translation="MRAHEAGALHADGYRGPTWLQKPADVNALHPSLWATSVGRSRDG
                     VLSVGGVSVTDLATQFGTPAYIVDEADFR"
     gene            complement(223..960)
                     /locus_tag="PLK64_08820"
     CDS             complement(223..960)
                     /locus_tag="PLK64_08820"
                     /inference="COORDINATES: protein motif:HMM:NF017555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HPV79890.1"
                     /translation="MADAVAEAAAYLSGMPATPVPLERTDRAPWVWASAQALRSRVNA
                     HGLADAVREHPAVEAVTVRADGWLEITLASEWVDRTLADLAAPAGMPPAHLPAAPPAV
                     LGRRTLDNPGFVVLLAHARACRVAGQALAGPERAGQATDQDDAGAFHPQALRDLSDPL
                     DVVLLTEILDAPRRLVATAPHQCAAALLAVAAAYLPWEERCPAIATRQGESSTPQHWA
                     RARISAAGLGVLERGMALLGITAPERI"
     gene            complement(972..1412)
                     /locus_tag="PLK64_08825"
     CDS             complement(972..1412)
                     /locus_tag="PLK64_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPV79891.1"
                     /translation="MEPTLGAGWGGIGVVLVCDDTESIRRLLRINLELDGFEVLEACD
                     GASAIALLRALQLEDRLPDVILLDAHMAPLDGWWALSEIRSCPRLSRIPVVMATADTV
                     CEDKAAMLARGLDACVQKPFDPEAVVDLVAGFVREGRAHVPQLM"
     gene            1505..1576
                     /locus_tag="PLK64_08830"
     tRNA            1505..1576
                     /locus_tag="PLK64_08830"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1538..1540,aa:Arg,seq:ccg)
     gene            complement(1658..2701)
                     /locus_tag="PLK64_08835"
     CDS             complement(1658..2701)
                     /locus_tag="PLK64_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPV79892.1"
                     /translation="MSHSMALWISLGLLLANAFFVGAEFAAMAARRSALEPLAAAGSA
                     RAATCLAALEQMGSMLATAQLGITVCSVGLGALAEAALHEVLHPVFAALPLGEAWANG
                     LSFAGALLIVVYLHVVAGEMIPKNLALAGPERAALVLVPALLMVSRVLRPVVAVMEWV
                     AKGLVRAIFRIEPKDEIASAFTVEEVTHILAESEREGLIEEHREGLVRSALEFSAKLA
                     ADVAVPVDRLVTVAKGVTPEDVERLVARTGFSRYPFLDKEGHVAGYLHLKDILYAADA
                     AARTERVPRKRLRRLATVAPTEEIEDVLATMRGNGTHLARVVDPTGEVIGVIFLEDIV
                     EELVGTISDAETR"
     gene            complement(2698..4158)
                     /locus_tag="PLK64_08840"
     CDS             complement(2698..4158)
                     /locus_tag="PLK64_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPV79893.1"
                     /translation="MGEWLLVLLAVILTVGTAIFVATEFALVALDRPTVQQAIDAGDT
                     RARGVLASLRRLSTQLSAAQVGITVTTLLVGYLAQPSIGHLLEAPLAAVLPAGSVESV
                     AAMLALVLVTLFSMVFGELVPQFLGISAPLPTAKVVAAPVRWFSMLAKPLIWVLNGSA
                     NGFLRGMGIEPQEELSGARTPQELASLVRRSALVGTMERETAERVTRTLDFGDRTAAD
                     VMTSRVRAVSIDRTASAADVVALARQTGHSRFPVVGDDWDDVDGIVHVKRALAVPPER
                     RVLVPVSALMVAHVTVPETIRLDALLVQLRQSGLQLAVVVDEYGGTAGVVTLEDVVEE
                     IVGDISDEHDWSGVSGRRAKDGSWSVPGTWRPDEVRSRLGAPVPDHAAYATLGGFLMA
                     ALGRVPERGDTVSLPGWKVRVQRMEGRRVARVRLVPVTPAVTDDPANATGREGDAGAP
                     GASRGPGVQRDPGSPRAPRGPLQPSQPASPPGEVTG"
     gene            4253..5515
                     /locus_tag="PLK64_08845"
     CDS             4253..5515
                     /locus_tag="PLK64_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPV79894.1"
                     /translation="MDFTPSPRSADLTARVRDFIDSRIDPVEATILADVQARRDRGEN
                     PWTVHPLIPELQALARTQGLWNLFLPAGHEGPDAARFGTDGGVGLSNVDYAPIAEQTG
                     RSTLAPLVFNCNAPDTGNMEVLLKYGSQEQKDTWLEPLLDGRIRSAFLMTEPDVASSD
                     ATTMAATARIEGDEVVLDGRKWWSTGVGHPDCAVGIFMGVTDPQAPKHARHSMVLVPL
                     DAPGVRIERVMPALGQWDEPLGHGEVSLTDVRLPASAIIGGPGRAFAIAQGRLGPGRV
                     HHCMRLIGLAERSLESACERASSRVAFGKPLANLGGNRERLAEARIAINQARLHVLHC
                     AWKLDAFGVEGAASEVSEIKAAVPRMACQVIDLAIQLHGGAGVSADTPLAAAYATARA
                     LRLADGPDEVHLGVVARHLLRPYQTDQR"
     assembly_gap    5521..5620
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5621..6650
                     /locus_tag="PLK64_08850"
     CDS             <5621..6650
                     /locus_tag="PLK64_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HPV79895.1"
                     /translation="WLRENATDARGLDGIPEVGQFGGGASNLTYLLRYAASPTAPAGR
                     DLVLRRPPAGTKAKGAHDMRREHDLQAALAPVFPLVPRMVACCQDHEVLGSDFYVMER
                     VAGIILRRNLPAELGLDADTTRRLCVAAVDTLADLHAVDVEAAGLAGLGRGPGYVGRQ
                     VAGWSERYRKARTPDVPDAEAVMAWLAAEQPPDLATVLIHNDFRFDNLVLDPTDPTRI
                     VGVLDWELATVGDPLMDLGSALAYWVQADDDLVMQRMRRQPTHAPGMLTRAEVVGRYT
                     ERAGLVVSPEQWRFYEAFGLFRLAVIAQQIYYRFVHGQTTNPAFAEFGAAVAYLDYRS
                     RSILKSRG"
     gene            6764..7792
                     /locus_tag="PLK64_08855"
     CDS             6764..7792
                     /locus_tag="PLK64_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPV79896.1"
                     /translation="MTTTTLSPPSGQPSSPGLPLEVRGLTKNFGSLVAVDHLDFSVEP
                     GRVTGFLGPNGAGKTTTLRMLLGLVRPTSGTATIGGSTYHDLARPLRTVGAALESSSF
                     HPGRSGRDHLRVLAAAGGIPTQRVDELLELVGIPAAARQRAGGYSMGMRQRLALAAAL
                     LGDPQVLLLDEPANGLDPEGIRWLRQFLRHLAAQGKTILVSSHMLSEVEQTVDDVVII
                     ANGRSVAQGSMDSLRGEPTVHVRAADDAALRSVLLSGGMLAEPSPEGGLTVKTHDLAR
                     VGELAFAGGIPLHELRALASDLEDLFFRLTSAPEHRNRNLGAAPTGAPPTGATPAGAS
                     LTQDGGSI"
     gene            7792..8589
                     /locus_tag="PLK64_08860"
     CDS             7792..8589
                     /locus_tag="PLK64_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV79897.1"
                     /translation="MGLAIRSEFRKFFTTRMWWGMGLAVIVASLAFPALYAFIFTSDG
                     MAEIGASAIPDSELAKTVYTGGIQLAYLLTLAIGVLTIGAEYRHKTITGTFLATPKRA
                     RVMLAKVVSLLGIGGLYGIVSVVAAFSIGAIILNAKGHAIWPDSSVGRTLALYLLVLG
                     LWALIGLGIGILIPNQIAALFIAIGVAWIVEPLLSVLLATQEWGKGIVRFFPSSATNA
                     VLGSAAQSGGGEAIPAFPWWGAALVLVAYAAIMAAVGTFLTLRRDVT"
BASE COUNT         1336 a         3091 c         2946 g         1246 t
ORIGIN      
        1 ccggaagtct gcctcgtcaa cgatgtaggc cggcgtcccg aactgcgttg ccagatcggt
       61 gaccgacacg cccccgaccg agaggacacc gtcgcgggaa cgccccacgc tcgtggccca
      121 caggctgggg tgcagggcgt tgacgtccgc cggcttctgc agccaggtcg gtccccggta
      181 gccatcggcg tgaagggcac cggcctcgtg tgcgcgcatg cctcatatcc tttcgggggc
      241 ggtgatcccg agcagcgcca ttccacgctc caagacgccc aatccggctg ctgagatgcg
      301 cgctcgggcc cagtgttggg gagtgctcga ctccccctgt cgggtcgcga tcgcggggca
      361 gcgctcctcc cagggcaggt atgccgccgc gaccgccagc agcgccgccg cacactggtg
      421 cggcgcggtg gcgaccaaac gacgcggggc atcgaggatc tcggtgagca ggaccacgtc
      481 gaggggatcg ctcagatcgc gcagggcctg cgggtggaag gcgcccgcgt cgtcctgatc
      541 ggttgcttgg ccggcgcgct ccggaccggc gagcgcctga ccggcgaccc ggcaggcccg
      601 ggcgtgggcg agcaggacaa cgaagccggg gttgtccaac gtccgccggc caaggacagc
      661 cggtggcgcg gctgggaggt gagcaggcgg catacctgcg ggcgctgcga gatcggcgag
      721 ggtgcggtcg acccactcgg aggcgagggt gatctccagc cagccgtccg cgcggaccgt
      781 caccgcctcg acggcggggt gctcacggac cgcatcggcc aacccatggg cgttcacgcg
      841 gctgcgaagg gcctgggcgc tggcccagac ccagggggcg cggtcggtgc gttcgagggg
      901 aacgggggtg gccggcatac cgctcaggta tgccgccgcc tcggcgaccg catcagccac
      961 acgcgccagt gtcacatcaa ctgcgggacg tgggcccgac cctcccggac gaaaccggcg
     1021 acgaggtcga cgacggcctc ggggtcgaac ggcttctgga cacaggcgtc caagccgcgg
     1081 gcgagcatgg ccgccttgtc ttcgcagacc gtgtcggccg tggccatgac gaccgggata
     1141 cgcgacagtc gcgggcagga acggatctcc gagagcgccc accacccgtc cagcggagcc
     1201 atgtgggcgt ccagcaggat gacgtcgggg aggcgatctt cgagctggag cgcgcgcagc
     1261 agggcgatgg ccgaggcgcc gtcgcatgcc tcaagaacct cgaagccatc aagctcaagg
     1321 ttgattctga gcagccgtcg gatggattcg gtgtcgtcgc aaacgagcac aacaccaatc
     1381 cccccccacc ctgcccccag cgtgggttcc acgttggtca gcctaggctg ataccctcct
     1441 ccaggcgtga caatcggcat catccactga tgagctgtcc acatgatgga caccgaacgt
     1501 tcaggccccc gtagctcagg ggatagagca ccgccctccg gaggcggggg cgcaggttcg
     1561 aatcctgccg ggggcacgta gtgagaaccg gtcgccgcac gagagtgcgg acggccggtt
     1621 ctcgcgtttc cgggtgtgtg gcggcttggc cgacgggtca gcgggtctca gcgtccgaga
     1681 tggtgccgac gagttcttcg acgatgtcct cgaggaagat caccccaatg acctcgccgg
     1741 tggggtcgac aacccgggcc aggtgggtgc cgttgccgcg catcgtcgcc agcacgtcct
     1801 cgatctcctc cgtgggggcg acggtggcca gacggcgcag ccgcttgcgg gggacgcgct
     1861 cagtgcgggc ggcagcgtcc gcggcataca ggatgtcctt gaggtgcagg tatcccgcga
     1921 cgtgaccctc cttgtcgaga aagggatagc gcgagaagcc ggtgcgggcg acgagccgtt
     1981 cgacgtcctc cggggtgacg cccttggcga cggtgaccag ccgatcgacg gggacggcga
     2041 cgtcggcggc cagcttggcc gagaactcca gcgccgagcg cacgagcccc tcgcgatgct
     2101 cctcgatcag cccctcgcgc tcgctctcgg cgaggatgtg ggtgacctcc tcgaccgtga
     2161 acgccgaggc gatctcgtcc ttgggctcga tccggaagat cgcccgcacc agccccttgg
     2221 cgacccattc catgacggcg accaccgggc gtagcacgcg cgacaccatg aggagcgccg
     2281 gcacgagcac gagggccgcc cgctccggcc cggcgagggc gaggtttttg gggatcatct
     2341 cgccggcaac gacgtgcaga tagacgacga tcaacagcgc accggcgaag gacagaccgt
     2401 tggcccaggc ctcccccagt ggcagagccg cgaagaccgg gtgcagcacc tcgtgcaggg
     2461 ccgcctcggc gagcgcgccc aggccgaccg agcacaccgt gatcccgagt tgggcggtgg
     2521 cgagcatcga gcccatctgc tccagggcgg cgaggcaggt cgcggctcgg gcgctgccgg
     2581 cagcggccaa cggctccagc gccgagcgtc gagccgccat cgccgcgaac tccgcgccga
     2641 cgaagaacgc attggccagg aggagcccga gggagatcca gagcgccatc gagtgactca
     2701 tcccgtcacc tccccaggtg gtgacgcggg ctggctcggc tgcagcggtc cgcgcggcgc
     2761 ccgaggactg cccggatctc gctggacacc cggccctcgg gaggcgcccg gcgcgcccgc
     2821 gtcaccttct cgccctgtgg cgttcgccgg atcgtcggtg actgccggcg tcaccggcac
     2881 gaggcggacc cgagccaccc gccgcccctc catccgctgc acccgcacct tccagccggg
     2941 cagcgacacc gtgtcaccac gttcggggac ccgccccaac gcggccatga ggaagccccc
     3001 aagcgtcgcg tatgccgcgt ggtccggcac gggggcaccg agccggctgc gcacctcgtc
     3061 agggcgccag gtgcccggca ccgaccagga cccgtccttg gcccgacgac cgctgacccc
     3121 cgaccagtcg tgctcgtcgg agatgtcgcc gacgatctcc tcgacgacgt cctcaagggt
     3181 gacgactccg gccgtgccgc cgtattcgtc gacgacgacg gccagttgaa gacctgattg
     3241 gcggagttgg acgagcaacg cgtcgaggcg gatggtctcg ggaaccgtca cgtgcgcgac
     3301 catgagcgcg gacaccggga ccaacacgcg acgctccggc ggcacggcga gggcccgctt
     3361 gacgtggacg atgccatcga cgtcatccca gtcgtccccg acgacgggga acctcgaatg
     3421 cccggtctgg cgggccaagg ccacgacatc ggcggcgctg gcggtgcgat cgatgctgac
     3481 ggcccgcacc cgtgaggtca tgacatcggc ggcggtgcgg tcgccgaaat ccaaggtgcg
     3541 agtgacgcgt tcggccgtct cgcgctccat cgtcccgacg agcgcggacc ggcggaccag
     3601 gctggccaac tcctgcgggg tgcgagcgcc ggacaactcc tcctgcggct cgatgcccat
     3661 cccgcgtagg aacccgttgg ccgagccgtt gagcacccag atgagcggct tggccagcat
     3721 cgaaaaccac cggaccggcg cggcaacgac cttggccgtc ggcaagggcg ccgagatgcc
     3781 gaggaactgc gggaccaact cgccgaacac catcgagaag agggtcacca gcaccagggc
     3841 gagcatcgcg gcgaccgact ccacggaccc ggccggcagg acggcggcca gcggcgcctc
     3901 gaggaggtga ccgatcgagg gttgggcgag gtaaccgacg agcagcgtcg tcacggtgat
     3961 cccgacctgg gccgcggaca actgcgtcga gagccgacgc agggaggcga gcactcctcg
     4021 ggcgcgggtg tcgccggcat cgatggcctg ttgaacggtc ggccggtcga gggcgaccag
     4081 ggcaaactcg gtcgcgacga agatcgcggt ccccacggtg aggatcaccg cgaggagcac
     4141 caacagccat tcgcccatag tcactccact gtaatgaacc gcgagcccta cggcggcggg
     4201 cgctcagggg agcggaagga agcgcgcatc gacgcggggt taaggtcggc ctgtggactt
     4261 caccccgtca ccgaggtctg ccgacctcac cgcccgcgtg cgggacttca tcgacagccg
     4321 gatcgacccc gtcgaggcca cgatcctcgc cgacgtccag gcccgccgtg atcgaggcga
     4381 gaacccgtgg accgttcacc ccctcatccc cgagttgcag gccctcgccc gcacccaggg
     4441 gctgtggaac ctcttcctcc cagccggcca cgagggtccg gatgccgcgc ggttcgggac
     4501 cgatggcggc gtggggttgt ccaacgtcga ctacgccccc atcgctgagc agaccgggcg
     4561 cagcaccttg gcccccttgg tcttcaactg caacgccccg gacaccggca acatggaggt
     4621 cctgctgaag tacggctcgc aggagcagaa ggacacctgg ttggagccac tgctggacgg
     4681 gaggatccgc agcgcgtttc tcatgaccga gccggacgtc gcgtcgtcgg acgcgacgac
     4741 gatggccgcc accgctcgga tcgagggaga cgaggtcgtc ctcgacgggc gcaagtggtg
     4801 gtcaaccggt gtggggcacc ccgattgcgc agtcggcatt ttcatgggcg tgaccgatcc
     4861 gcaggccccg aagcacgctc gacactcgat ggtcctcgtc ccgctcgacg cccccggcgt
     4921 gcggatcgag cgcgtgatgc cggccctggg gcaatgggac gaacccctcg gccacggtga
     4981 ggtctcgctc accgacgtcc gactgcccgc ctcggcgatc atcggcggcc ccggtcgggc
     5041 cttcgccatc gcccagggcc ggctcggtcc cggccgggtg caccactgca tgcggctcat
     5101 cggcctggcc gagcgctccc tcgaatcggc ctgcgaacga gcatccagcc gcgtggcctt
     5161 cggcaagccg ttggccaacc tcggcggcaa ccgcgagcgc ctggcggaag cgcgcatcgc
     5221 catcaaccag gcccgactgc acgtcctgca ctgcgcctgg aaactcgacg ccttcggggt
     5281 cgagggcgcc gcgtccgagg tgagcgagat caaggccgcc gtcccccgga tggcatgcca
     5341 ggtcatcgat ctggccatcc agttgcacgg cggcgccggc gtcagcgccg acaccccgct
     5401 ggcggcggca tacgcgacgg ctcgggcgct gcgcctcgcc gacgggccgg acgaggtcca
     5461 cctcggggtc gtcgcccgcc acctgttgcg gccctaccag acggaccagc ggtgacggcc
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctggctgcgc gagaacgcca
     5641 cggacgcaag ggggttggac ggcataccgg aagtcgggca gttcggcggc ggggcgtcta
     5701 acctcaccta cctccttcgg tatgccgcct cccccaccgc cccggcaggc cgcgatctcg
     5761 tcctgcgccg accgccggcg ggcaccaagg ccaagggtgc gcacgacatg cgccgtgagc
     5821 acgacctgca agcggcgctg gcccccgtct tcccgctcgt cccgcgcatg gtcgcctgct
     5881 gtcaggacca cgaggtcctc ggcagcgact tctacgtcat ggagcgggtg gcgggaatca
     5941 tcctgcggcg caacctgcct gcggagttgg ggctcgacgc cgacaccacc cggcggttgt
     6001 gcgtcgccgc ggtcgatacc ctggccgacc tgcatgccgt cgatgtcgaa gccgccgggc
     6061 tggcgggact cggccgtggc cctggctatg tcgggcgaca ggtcgccggc tggtccgagc
     6121 gctaccggaa ggcgcgcacg cccgacgtcc cggatgccga agcggtcatg gcgtggttgg
     6181 ctgccgagca accccccgac ctggcaacgg tgttgatcca caacgatttc cgcttcgaca
     6241 acctcgtcct cgacccgacc gatccgaccc ggatcgtcgg cgtcctggac tgggagttgg
     6301 ccaccgtcgg cgacccgctg atggacctgg ggtcggcgct ggcctactgg gtgcaggccg
     6361 acgacgacct cgtgatgcag cggatgcggc ggcagcccac gcacgccccg ggaatgctca
     6421 cccgggccga ggtcgtcggg cgctacaccg agcgcgccgg gctcgtcgtg tcgcccgaac
     6481 agtggcgttt ctacgaagct tttggcctgt tccggctggc tgtcatcgcc caacagatct
     6541 actaccgatt cgtccacggg cagacgacca acccagcctt cgccgagttc ggagcggccg
     6601 tcgcctacct cgactaccgc tcccggtcga tcctcaagag ccggggttga ccctggcccg
     6661 accctgatcg gcggctgaaa accgcgtgtt cgactggcac cgaggtcgcc gggcaggttt
     6721 ggatggaggg tatgactact actcagggga cgatcgaccg acgatgacga ccacgaccct
     6781 ctctcccccc agcggacaac catccagccc cggcctccct ctggaagtgc gcggactcac
     6841 caagaacttc ggcagcctgg tggccgtcga ccacctcgac ttcagcgtcg agcccggccg
     6901 cgtcaccggc ttcctcgggc ccaacggagc cggcaagacg accactctgc ggatgctgct
     6961 cgggctcgtg cgtcccacct ctggcaccgc caccatcggc gggtcgacct accacgacct
     7021 cgcccgcccg ctacgcaccg tcggggcagc gctcgaatcg agcagcttcc accctggtcg
     7081 gagcggccgc gaccacctgc gggtgctcgc cgcggccggc ggcatcccga cccagcgggt
     7141 cgatgagttg ctcgaactcg tcggcatccc ggccgccgct cggcagcgcg ccggtggcta
     7201 ctcgatgggc atgcgccagc gtctcgcgct cgccgcagcc ctgttgggtg acccgcaggt
     7261 cctgctgctc gacgagcccg ccaacggtct ggaccccgaa gggatccgtt ggctgcgcca
     7321 gttcctgcgc cacctcgcgg cgcagggcaa gacgatcctc gtctcgagcc acatgctctc
     7381 cgaggtcgag cagaccgttg acgatgtcgt catcattgcc aacggacgca gcgtcgccca
     7441 agggtcgatg gacagcctgc gcggtgagcc caccgtgcac gtgcgcgccg ccgacgacgc
     7501 ggccctgcgc agcgttctgc tcagcggcgg aatgctcgcc gagccgtccc cggagggcgg
     7561 cctgaccgtg aagacccacg acctcgcccg cgtcggggag ctcgcgttcg cgggcggcat
     7621 acccctgcac gagctgcgcg ccctcgccag tgacctggag gatctgttct tccggctcac
     7681 ctcggcgccc gaacaccgca accgcaacct cggggcagca ccgaccggcg cgccccccac
     7741 cggtgccacc cctgctgggg catccctgac tcaggacgga gggtcgatct gatgggcctc
     7801 gccatccgtt ccgagttccg taagttcttc accacccgga tgtggtgggg catgggcctg
     7861 gccgtcatcg tcgccagcct ggccttccct gctctctacg ccttcatctt caccagcgac
     7921 ggcatggccg agatcggcgc gtcggcgatc ccggactctg agttggccaa gaccgtctac
     7981 accggtggca tccagctcgc ctatctgctg accctggcga tcggcgtgct caccatcggg
     8041 gccgaatacc ggcacaagac gatcaccggc accttcctgg ctacccccaa acgcgcccgg
     8101 gtgatgcttg ccaaggtggt ctcgctgctc gggatcggtg gcctctatgg gatcgtctcg
     8161 gtggtcgcgg ccttctcgat cggcgcgatc atcctcaacg ccaaggggca cgcgatctgg
     8221 ccggattcct cggtcggccg gaccctggcg ctctacctgc tcgtcctggg cttgtgggcc
     8281 ctcatcgggc taggcatcgg catcctcatc cccaaccaga tcgctgccct cttcatcgcg
     8341 atcggcgttg cctggatcgt cgagccgttg ctcagcgtgc tgctcgccac tcaggagtgg
     8401 ggaaagggca tcgtccggtt cttcccgtcc agcgcgacca acgccgtgct cggctccgcc
     8461 gcgcagtccg gggggggaga ggcgataccc gcgttcccct ggtggggtgc cgcgctggtc
     8521 ctcgtcgcct atgccgccat catggcggcc gttgggacat tcctcacgct tcggcgcgac
     8581 gtcacctgag cggtgccgga ccccgtccgg tatgccgttc ttgcgccccc ggtcgaccgc
     8641 gtcagcacgc ggccgaccgg gggcgtatcg catggcgagg cgggtaggtg cgcagaagcg
     8701 gcccaaggta gcctggacc
//
LOCUS       DAOMZY010000134         8576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_7767_length_8576_cov_6.189766, whole genome shotgun sequence.
ACCESSION   DAOMZY010000134 DAOMZY010000000
VERSION     DAOMZY010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 8576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8576
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7767_length_8576_cov_6.189766"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="PLK64_08865"
     CDS             <1..1015
                     /locus_tag="PLK64_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPV79898.1"
                     /translation="LGPLLHDRAEFRLPYAGGCDLGTRTVEPHLAALRSFGLNVVATH
                     GFYEAHAAPHQPDKPIILTERGDTVTENVLLAAARNPGTTTIRNASPNYMVQDLCFFL
                     LKLGVQIEGIGSTTLKVTGVADIDVDVEYSPSEDPIEAMSLLAAAVVTESELTIRRVP
                     IEFMEIELATLEGMGMQVKMTPEYLSANGHTRLVDITTIPGPLKAPIDKIHPLPFPGL
                     NIDNLPFFALIAAMAEGTTMLHDWVYDNRAIYLTELTKVGAKVQLLDPHRVLIEGPTK
                     WRAAEVMCPPALRPGVVVLLAMLAAPGTSVLRNIYVINRGYEDLADRLNNLGATIEVF
                     RDI"
     gene            complement(1087..2484)
                     /locus_tag="PLK64_08870"
     CDS             complement(1087..2484)
                     /locus_tag="PLK64_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HPV79899.1"
                     /translation="MSFLIIIAALAFLMWMAYRGHSVILFAPIAALAAVLLTDPSLVA
                     PMFTGLFMDKMVGFIKNYFPVFLLGAIFGKVIEMSGFARAIVTWVIKVVGAKRAILSI
                     VLVCALLTYGGVSLFVVVFAVYPFAAEMFRMGDIPKRLIPGTIALGAFTFTMDALPGT
                     PQIQNIIPTTFFKTNAYAAPILGFVGAIFVFSLGMLYLERARRKALAKGEGYGEGHIN
                     EPEVFSGERKVNPALAVLPLVLVAVVNKVLTSKIPEWFGDKVSFSPTVVGKSDPVTVA
                     VSSNVAIWAVEGALLIGILTVIALAWKPVSSHFAHGSKEAVGGAMLAATNTASEYGFG
                     AVIAALPGFLVVANGLAKIPNTLVNEAVTVNVLAGVTGSASGGLSIALGAMANTFVEN
                     ATAAGIPMEVLHRVASMASGGMDTLPHNGAVITLLAVTGLTHKVSYKDIFAMTIIKTV
                     AVFVVIGFYYLTHLV"
     gene            complement(2568..2696)
                     /locus_tag="PLK64_08875"
     CDS             complement(2568..2696)
                     /locus_tag="PLK64_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79900.1"
                     /translation="MKADLRVDGWGAEGAGSGAARAQSITPAYSSGMWAAYMLDGR"
     gene            2695..4356
                     /locus_tag="PLK64_08880"
     CDS             2695..4356
                     /locus_tag="PLK64_08880"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HPV79901.1"
                     /translation="MTPIASPAPGSAAPGSVASGPHTSLVRSVESAMELIRSGETIAT
                     SGFVGCGTPDALLDGLARRFRESGDPAGLTLLFAAGQGDGGEQGLNRLAEPGLLRRVV
                     GGHWGLIPRVAALAVSGQIEGWNLPQGVISRLYRDMAAGLPGTVSRIGLHTFVDPRSQ
                     GGRVNERTTDDLVEVVHLGGEELLFYRATPIDVALLRGTSADEHGNITMEREALTLDN
                     LAMACAARNNGGKVIVQVERIVSAGTLDPRLVKIPHILVDAVVVAPPELHRQTYATAF
                     NPGYAHEVRIPLDLIPPMPMNERKIAARRAAMELPDGGVVNLGIGMPDGVSTVAAEEH
                     VIDRITLTTEPGIVGGMPASGGDFGAALNPDALIDQNQQFDFYQGGGLDLTVLGMAEL
                     DADGNVNVSRFGPRLTGAGGFIDISQSARTVVFVGTFTAGGMQVGISDGRLTILSEGR
                     SRKLVRHVEQVTFNGRYAASLGQRVLYVTERAVFAATPEGLRLIEVAPGIDIERDVLA
                     QMDFAPIVQDVALMDSRIFLPQPMGLAALLRPRTTSTPTLEGVSA"
     gene            4353..5213
                     /locus_tag="PLK64_08885"
     CDS             4353..5213
                     /locus_tag="PLK64_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPV79902.1"
                     /translation="MTTHPMTGRVVLITGAAGGLGRAHAHAFAALGAHVVLSDVTDPA
                     AVVAEVTAAGGSALGVVAGVDTWEGAAVSVAAAVEAFGRLDVLVNNAGIIRDKSFAKA
                     TPEMIEAVLAVHLHGAFHMTKAAWPHLTERGGSIINTTSGSGLYGNFGQVNYAAAKAG
                     LVGFTRSLALEGARAGIRANAIAPLAKSAMTETMMAPELLERLAPEWVSPLVTWLASD
                     GCTATGNVYSVGAGRYARVATLEAPGVRFDHVPTIAELTEAAAAIDDLAGAVEPRSLA
                     DQIALVAPPA"
     gene            5260..7128
                     /locus_tag="PLK64_08890"
     CDS             5260..7128
                     /locus_tag="PLK64_08890"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV79903.1"
                     /translation="MAQDGSLELLRLLADSAPTHHLDRIGAQPEAHRLARAVALRAED
                     QRRREGQLRALVDTARDLAAARDPSGVLDAIVRRARALMGTDVSYLTLYDPERGDTYM
                     RATDGAVAPTFRALRLELGVGLGGLVASSLSPWWSTDYPHDDRFAHTTTIDTGVQDEG
                     LVAICGTPLVAEGEFVGVLFAAHRTRHTFTQEEVALLGSLADLAAVSMVQARAHQETA
                     RALAALSVAHEAVRRHTEVIERSAAAHDRFTELIGAGGGVTDLTTALVGLVGGWAVLT
                     DAAGTRRSAHGTPPGDPVEAGAVDPLADTPLARAARASGGLTVEDMRYAVPVRAGHHH
                     LGTLVGVAGQLHDADWRIVERAAAVSALVLLFERDTEAARQRRVSDLVTDLLAGVLPS
                     AEATRVLRKEGLDAGVPLCVIAMRATTAPVSALVLAAGVAVGRSGIVGEAGRTAVALV
                     ADADPSAAASRLAARLRSFGEVTAAGVGPVAGMDGIPDAFGEARRTLDAMLALDRRGE
                     SAAAADLGFAGLVVGSAPDIAAYVERVIGPVIDYDRERGAELVKTLEAYFAAGGSAAR
                     ASEPLHVHVNTVVQRMSRVGRLLGADWDEPERALEVQLALRLRRLLPTRPRSRPSG"
     gene            complement(7172..7594)
                     /locus_tag="PLK64_08895"
     CDS             complement(7172..7594)
                     /locus_tag="PLK64_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79904.1"
                     /translation="MDSAPISSGSGTPQDPWRLKTPPLSSDYTMHKDVRDGVEVIVCT
                     VGKTVLLYDGRCVADLHAMLTAQGDWVELGSADEQKPAKEGTVEAWGRSADNPVGGWY
                     GLKKGLRGRFGMYVPPLLEHLGLAEVEHNPRGNRMRAI"
     gene            complement(7654..8535)
                     /locus_tag="PLK64_08900"
     CDS             complement(7654..8535)
                     /locus_tag="PLK64_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPV79905.1"
                     /translation="MTFHVHDLSVGPIADEAPVVIAVHGITANGMCWPLLADELAGRR
                     PGVRFLAPDLRGRAESPADDDHAGLDVHVADLVALAREHGGRAIFVGHSMGAFVCALL
                     AATHPDVAAGVVLVDGGLSFPFPPGLDIDATLAAALGPSLARLSMTFADEDELLTFVS
                     TNPALGSLLDGEFGEAARTYLRHDIVRGEDGLVRSSCSLDAVRADGRGMLVHPRLPGA
                     VREAVDAGVPMELLWAPRGLFDEPQGMYDEERLAVLGLPEALRVTQVPDTNHFSILLG
                     AEGIAAIGDAVERHLDA"
BASE COUNT         1331 a         3087 c         2856 g         1302 t
ORIGIN      
        1 cctcgggccg ctgctgcacg accgcgccga gttccgactg ccgtatgccg ggggctgcga
       61 cctcggcacc cgcaccgttg agccgcacct ggcggcgctg cggtcgttcg ggttgaacgt
      121 cgtggcgacc cacgggttct acgaggccca tgccgcccct caccagcccg acaagccgat
      181 catcctcacc gagcgtggcg acacggtcac cgagaacgtc ctgctggcgg ccgcccgcaa
      241 cccgggcacg acaacgatcc gcaatgccag ccccaactac atggtgcagg acctgtgctt
      301 cttcctgctc aagctcggcg tgcagatcga ggggatcgga tcgacgaccc tcaaggtcac
      361 cggtgtggcc gacatcgacg tcgacgtcga gtactccccc agcgaggacc ccatcgaggc
      421 gatgtccctg ttggcggccg cagtggtgac cgagtcggag ttgacgatcc gccgcgtgcc
      481 gatcgagttc atggagatcg agctggccac gctcgaaggc atgggcatgc aggtcaagat
      541 gacccccgag tacctctcgg caaacggcca cacccgtttg gtggacatca caacgatccc
      601 cgggccgctg aaggcgccga tcgacaagat ccacccgctg ccgttccccg gcctcaacat
      661 cgacaacttg ccgttcttcg cgctcatcgc cgcgatggcc gagggcacga cgatgttgca
      721 cgactgggtc tatgacaacc gcgccatcta cctgaccgag ctcaccaagg tcggcgccaa
      781 ggtgcagttg ctcgacccgc accgcgtgct catcgaaggg ccgacgaagt ggcgcgcggc
      841 cgaggtgatg tgcccaccgg cactccgccc aggcgtcgtc gtcctgctcg cgatgctggc
      901 cgccccgggg acctcggtgc tgcgcaacat ctacgtcatc aaccgcggct acgaagacct
      961 ggccgaccgc ctcaacaacc tcggggcgac catcgaggtc ttccgcgaca tctgaccgca
     1021 gccagaccga gcgagcagac caagcggggc cggaccactg ggtggtccgg ccccgcttcg
     1081 tcgccgtcag accaggtggg tgaggtagta gaagccgatg acgacgaaga ccgccacggt
     1141 cttgatgatc gtcatggcga agatgtcctt gtaggacacc ttgtgggtca ggccggtcac
     1201 agcaagcagg gtgatgacgg ctccgttgtg cgggagcgtg tccatgccgc cgctggccat
     1261 cgaggccacc cggtgcagga cctccatggg tatgccggcc gccgtcgcgt tctcgacgaa
     1321 ggtgttggcc atagcgccga gggcgatcga caggcctccg gaggcggagc cggtgacgcc
     1381 ggcgagcacg ttgaccgtga cagcctcgtt gacgagcgtg ttggggatct tggccaggcc
     1441 gttggcgacg acgaggaacc cgggcagggc agcgatgacc gccccgaagc cgtattccga
     1501 cgcggtgttc gtcgcggcga gcatcgcccc tccgacggcc tccttggacc cgtgggcgaa
     1561 gtggctggac accggcttcc aggcgagggc gatgacggtg aggatgccga tgagcagcgc
     1621 gccctcgacc gcccagatcg ccacgttgct ggagactgcg acggtgacgg ggtcggactt
     1681 gccgacaacg gtcggcgaga agctcacctt gtcgccgaac cactcgggga tcttcgaggt
     1741 aaggaccttg ttgacgacgg cgacgagaac gaggggcagc acggcgaggg ccgggttgac
     1801 cttgcgctcg ccgctgaaga cctcgggctc gttgatgtgc ccctcgccat agccctcgcc
     1861 cttggccaac gccttgcgcc gggcccgctc gaggtagagc atcccgaggc tgaagacgaa
     1921 gatcgcaccg acgaagccga ggatgggggc ggcatacgcg ttcgttttga agaaggtcgt
     1981 cgggatgatg ttctggatct gcggggtgcc aggcagcgcg tccatcgtga aggtgaacgc
     2041 gccgagagcg atcgtgccgg ggatgagtcg cttggggatg tcgcccatgc ggaacatctc
     2101 ggccgcgaag gggtagacgg cgaacacgac cacgaacagc gagacgccac cgtaggtgag
     2161 cagcgcgcag acgaggacga tcgagaggat cgcgcgtttg gcgccaacga ccttgatgac
     2221 ccaggtgacg atggcccggg cgaagccgga catctcgatg accttcccga agatcgcgcc
     2281 aaggaggaac accgggaagt agttcttgat gaagccgacc atcttgtcca tgaacaggcc
     2341 cgtgaacatc ggggcgacga gggacgggtc ggtgagcagg accgcggcga gagcggcgat
     2401 cggcgcgaag aggatgacgc tgtgcccgcg ataggccatc cacatgagga aggccaacgc
     2461 ggcaatgatg atgaggaagc tcatggctct cccggaggct gcggggtcag aggggatgtc
     2521 ttcatcgtgg cgaggcacgg cgtcgccgcc gatgtgtgga tccaccatta ccggccgtcg
     2581 agcatgtatg ccgcccacat gccagacgag tacgcagggg tgatggactg agcgcgagcc
     2641 gcaccgctgc ccgcgccttc cgcgccccac ccgtccactc ggaggtccgc cttcatgacc
     2701 cccatcgcct cccctgctcc cggatccgct gctcccggat ccgtcgcctc gggacctcac
     2761 acctcactcg tccggtcggt cgagtccgcc atggagctca tccgcagcgg cgagaccatc
     2821 gccacgtcag gcttcgtcgg ctgcggcacc cccgacgccc tcctcgacgg gctggctcgg
     2881 cgcttccgtg agagcggcga cccggccggg ctcaccctgc tcttcgcggc cggacaaggt
     2941 gacggaggag aacagggcct caaccggctc gccgagccgg gcctgctgcg ccgggtcgtc
     3001 ggtggccact gggggctgat tccccgtgtg gcggctctcg ccgtcagcgg tcagatcgag
     3061 gggtggaacc tcccccaggg cgtcatcagc cggctctacc gcgacatggc cgccggcctg
     3121 cccggcacgg tctctcggat cggtctgcat accttcgtcg accctcgctc ccagggtggc
     3181 cgagtcaacg agcgcacgac tgacgacctt gtcgaggtcg tgcatctcgg cggtgaggag
     3241 ctgctgttct accgcgccac ccccatcgac gtcgccctcc tgcgcggcac ctccgccgac
     3301 gagcacggca acatcaccat ggagcgcgag gcgctcaccc tcgacaacct cgccatggcc
     3361 tgcgccgccc gcaacaacgg cggcaaggtc atcgttcagg tcgagcggat cgtttccgcc
     3421 gggaccctcg acccgcgctt ggtcaagatc ccccacatcc tcgtcgatgc cgtcgtcgtc
     3481 gctccccccg agctgcaccg gcagacctac gcgaccgcct tcaaccccgg ctatgcccac
     3541 gaggtgcgca tcccgttgga cctcatcccg ccgatgccga tgaacgagcg caagatcgct
     3601 gcccgccggg cggccatgga gttgcccgat ggcggcgtcg tgaacctcgg gatcggtatg
     3661 ccggacggcg tgtcgacggt ggccgccgag gagcacgtca tcgaccggat caccctcacc
     3721 accgagccgg ggatcgtcgg cgggatgcct gcctcgggcg gcgacttcgg cgcggcgctc
     3781 aacccggacg ccctcatcga ccagaaccag cagttcgact tctaccaggg cggcggcttg
     3841 gacctcacgg tcctcggaat ggccgagctc gacgccgacg gcaacgtcaa tgtctcgcga
     3901 ttcggcccgc ggctcaccgg tgccggtggc ttcatcgaca tcagccagag cgcccggacc
     3961 gtcgtcttcg tcggcacctt caccgccggc ggcatgcagg tcggcatctc cgacggacgg
     4021 ctgacaatcc tctccgaggg ccgcagccgc aaactggtcc ggcacgtcga gcaggtcacc
     4081 ttcaacggtc ggtatgccgc gtcgctgggt cagcgggtcc tctacgtcac tgagcgcgcc
     4141 gtcttcgccg ccacccccga gggactgcgg ctcatcgagg tcgctcctgg catcgacatc
     4201 gagcgcgatg tcctcgctca gatggacttc gcaccgatcg tgcaggacgt cgccctcatg
     4261 gactcgcgga tcttcctccc tcagcccatg ggtctggccg ccctcctccg accccgcacc
     4321 acatccaccc ccacccttga aggagtctcc gcatgaccac tcacccgatg accggacgcg
     4381 tcgtcctcat caccggagca gccggcggcc tcggccgggc ccacgcccac gcgttcgccg
     4441 ctctcggcgc acacgtggtg ctcagcgatg tcaccgaccc agccgcggtc gtcgccgagg
     4501 tgacggccgc cggagggtcg gccctcgggg tcgtcgccgg ggtcgacacc tgggagggcg
     4561 ccgcggtgtc cgtcgcggcc gcggtcgagg ctttcgggcg cctcgacgtt ctggtcaaca
     4621 acgcggggat catccgcgac aagagcttcg ccaaggccac ccccgagatg atcgaggccg
     4681 tcctcgccgt ccacctgcac ggggccttcc acatgaccaa ggccgcctgg ccgcacctca
     4741 ccgaacgggg cggctcgatc atcaacacga cctcgggttc gggtctctac ggcaacttcg
     4801 gtcaggtcaa ctacgccgcc gccaaggccg gtctcgtcgg cttcaccagg tccctcgcgc
     4861 tggaaggtgc gcgggccggc atacgcgcca atgccattgc gccactggcg aagtcagcga
     4921 tgaccgaaac gatgatggcc cccgaactgc tcgagcgact cgcccctgaa tgggtgtcgc
     4981 cgctcgtcac ctggctcgcg agcgacggct gcacggccac tggcaacgtc tattcggtgg
     5041 gcgcgggtcg ctatgcccgg gtcgccaccc tcgaggcacc gggcgtgcgc ttcgaccacg
     5101 ttccgacgat cgctgagctg accgaggcag ccgccgccat cgacgacctc gccggcgcgg
     5161 tcgagccgcg ctcgctggcc gaccagatcg ccctcgtcgc gccacccgcg tgagctctca
     5221 gccgccgcgc gcggtcgccg ctcggtaggc tcgacggtcg tggcgcagga cgggtccctc
     5281 gaactgctgc ggttgctcgc cgacagcgcg ccgacccatc acctcgaccg gatcggcgcg
     5341 cagcccgagg cgcaccggtt ggctcgcgcg gtcgccctgc gcgccgagga ccagcgccgt
     5401 cgcgagggtc aacttcgagc cctcgtcgac accgcccggg atctggcggc ggcgcgcgac
     5461 ccgtcggggg tgctcgatgc gatcgttcgt cgggcccggg ccctcatggg caccgacgtc
     5521 tcgtatctca ccctctacga tcccgagcgc ggcgatacct acatgcgggc gaccgacggc
     5581 gccgtcgccc cgaccttccg cgcgctgagg ctggagctcg gggtcgggct cggcgggttg
     5641 gtcgcctcgt ccctctcgcc gtggtggagc accgactacc cccacgacga ccgcttcgcc
     5701 cacacaacga cgatcgacac gggggtccag gacgaaggtt tggtcgccat ctgcggcacc
     5761 ccgctagtcg ccgagggcga gttcgtcggg gtcctcttcg ccgcgcatcg gacgcggcat
     5821 accttcacgc aggaggaggt cgccctcctc gggtcgctcg ctgacctcgc ggccgtctcg
     5881 atggtccagg cgcgggccca tcaggagacg gcgcgagcct tggcggcgtt gtctgtcgcc
     5941 cacgaggcgg tccggcggca taccgaggtc atcgagcgct cggcggcggc ccacgaccgg
     6001 ttcaccgaac tcatcggcgc cggcggtggg gtcactgatc tcacgaccgc cctcgtcggc
     6061 ctcgtcggtg ggtgggcagt tctcaccgat gccgcgggaa cccgccgcag cgcccacgga
     6121 acacctcccg gcgaccctgt cgaagccggt gcggtcgatc ctctggccga cacgccgctc
     6181 gcgcgcgcgg ctcgggcgtc cggggggctc accgtcgaag acatgaggta tgccgtgccc
     6241 gtccgggccg gccaccacca tctcgggacg ctcgtcggcg tggccggcca acttcacgac
     6301 gctgattggc gcatcgtcga acgagccgca gcagtgtcgg ctctcgtcct gctcttcgag
     6361 cgcgacaccg aagcggcccg gcagcgccga gtctccgatc tcgtgacaga tctgctcgcc
     6421 ggggtgctgc cgtcggccga ggcgacccgg gtgctgcgca aagaggggct cgatgcgggc
     6481 gtgccgctct gtgtcatcgc catgcgggcc actaccgctc cggtgtcggc actcgtgctc
     6541 gccgcgggag tggccgtggg acgttcgggg atcgtcggcg aggccgggcg caccgccgtg
     6601 gccctcgtcg ccgacgccga cccgagcgcg gcagcgtccc ggctcgccgc tcggctgcgc
     6661 tccttcggtg aagtgaccgc cgcgggagta ggccccgtgg cggggatgga cggcataccg
     6721 gatgcctttg gggaggcccg ccgaaccctt gatgcgatgc tcgccctcga ccggcgcggg
     6781 gagagcgccg ccgctgccga cctgggcttc gctgggctcg tcgtcggctc ggctccggac
     6841 atcgccgcct acgtggagcg ggtcatcggc ccggtgatcg actacgaccg cgagcgcggg
     6901 gcagagctcg tcaagaccct ggaggcctat ttcgccgcag gcggcagcgc ggcgcgggcg
     6961 agcgagccgc tgcacgtgca cgtcaacacc gtcgtccagc ggatgagccg cgttggtcgg
     7021 ctccttggcg ccgactggga cgagccggag cgcgcccttg aggttcaact cgccctgcga
     7081 ctgcgccgac tgctccccac ccggccgcgc tcccgcccct cgggttgagc cggcggtctc
     7141 gcgctgaggt aggcggcccc gccgggtcag gctagatcgc gcgcatccga tttccgcgag
     7201 ggttgtgttc aacctcggca aggccgagat gttccagcag cggcgggacg tacatcccga
     7261 accgcccccg cagccccttc ttgagcccgt accagccacc cacgggattg tccgcagacc
     7321 gaccccacgc ctccacagtg ccttccttgg ccggcttctg ctcgtccgcc gagcccaact
     7381 ccacccagtc cccctgcgcc gtcagcatcg cgtgcaggtc ggccacgcac cgaccgtcgt
     7441 agaggagcac cgtcttgcca acggtgcaga cgatgacctc gactccatcg cgaacgtcct
     7501 tgtgcatcgt gtagtcggaa ctcaacggcg gggtcttcaa ccgccatggg tcctggggtg
     7561 tgccactgcc gctgctgatc ggtgctgagt ccatcgccgc agtatgccgc gtctgcctcg
     7621 tcaggatcgc cgccgcgcca ccaatcggag cgatcaggcg tcgaggtgcc gctcgacggc
     7681 atcgccgatc gccgcaatgc cctcggcacc cagcaggatg ctgaagtggt tggtgtcggg
     7741 cacttgggtc acccgaagag cctcgggcag cccgagcacc gccagtcgct cctcgtcata
     7801 catgccttgc ggctcgtcga agagccctcg gggcgcccaa agcaactcca tcggcacacc
     7861 cgcgtccacc gcctcacgca cagctccagg caatcgcggg tggaccaaca tgccgcgtcc
     7921 gtcggcgcgc accgcgtcga gcgaacacga actgcgcacg agcccgtcct cgccgcgcac
     7981 gatgtcgtga cgcaggtagg tgcgcgcggc ctcgccgaac tcgccgtcca gcagcgaccc
     8041 taacgcgggg ttcgtggaca cgaaggtgag caactcgtcc tcgtcggcga aggtcatcga
     8101 cagtcgcgcc agcgatgggc cgagcgccgc cgccaacgtc gcgtcgatgt ccaggcccgg
     8161 cgggaagggg aaggacagcc cgccgtcgac cagcaccacg ccagcagcca catcggggtg
     8221 cgtcgcggcc agcaacgcgc agacgaacgc ccccatcgaa tgcccgacga agatcgcccg
     8281 accgccgtgc tccctcgcca gcgcgaccag gtcggcgacg tggacgtcga gcccggcgtg
     8341 gtcgtcgtcg gccgggctct cggcccgtcc ccgcagatcg ggtgccaaga accgcacccc
     8401 gggtcgtcgc ccggccaact cgtcggcgag aagcggccag cacattccgt tggccgtgat
     8461 cccgtggacg gcgatgacaa cgggcgcctc gtcggcgatc ggcccgaccg acaggtcgtg
     8521 gacgtggaag gtcatgcctg cattgtggcg gtgatccggt cggcgatcgc ctccgg
//
LOCUS       DAOMZY010000135         8559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_7787_length_8559_cov_6.310207, whole genome shotgun sequence.
ACCESSION   DAOMZY010000135 DAOMZY010000000
VERSION     DAOMZY010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 8559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8559
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7787_length_8559_cov_6.310207"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="PLK64_08905"
     CDS             <1..806
                     /locus_tag="PLK64_08905"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV79906.1"
                     /translation="FDDAGVITGGDPSSPLRFIVPVAVAVATSMILVVALRPIDRHIQ
                     RSQVPPYSVGEFFGSFVFNPRKNPDFTWAWVSRFMFMLGIAYLLVYQAPYSSAHLKFE
                     GADLDRVVLWGTVVTVVGTVLTGYVAGQLSDRFKRRKPFVIGSAMGYAVSLVFLSLAA
                     PDNSGLPMALVGLFLGGLAQGVYFGIDLALVTDVLPDREADAAKDLGVFNIASAGPQF
                     IAPFIAPVFLGLNLLGSGANQDNFAALFVFAAIFSAIGALLVLPIKAVR"
     gene            814..3336
                     /locus_tag="PLK64_08910"
     CDS             814..3336
                     /locus_tag="PLK64_08910"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HPV79907.1"
                     /translation="MSAPEAPSTTLADPAPTLADRRQPGAGPATTGNNGLIDELLGRL
                     TLEQKVELITGASFWTTAACPEIGLRSLTLSDGPAGVRGRFWSEQHPSAALPNAVCLA
                     ATFDPELVERAGRLIGSEARRNGVDVLLGPTVNLHRSPHGGRNFEAFSEDAVLTAQTG
                     GALVDGIQSQGVAATAKHFIGNDSETERMTLDAVIDEATLRSTYALPFEELVVDHDVA
                     AVMSSYNSINGTSGTESPLLESLLRAEWGFASLVISDWGAVRSVEAAAAARTDLAMPG
                     PESPWSEGLVAAVRAGRADEADVDVKVASLLGLAARLGALSGIPPRQDPTALDDPHDP
                     SSPAVRDLLATIAARGAVLLRNEGATLPLEPSPASIAVIGPAATRLRVGGGGSATVVP
                     PHVVSVVTGIEAAFPDALVHAVEGVTQHRLLRPIGDRSTNDLGAAGYDVELLDLEGTV
                     LAAQARTGGELIYGMGFPDGVDGDLVTRIRVTMTLHETGPRRIGVAGIGDAEIWIEGQ
                     RVAAQTLRNEHDDEIAGLSRPPQLVLDISLTGGERVVIEFAPAPDTIAALRIGLEGPA
                     RSTADLLEEAVAAARAADVAVVVVGTTEAEESEGFDRESLSLGADIDALVEAVAAVNP
                     RTVVVLNVGAPVLLPWRERVAAILLAHFPGQEGGTAIGRILAGAEEPGGRLPSSWPRT
                     ADSRVPSVTPVAGTLAYQEGEAFGYRDPEGDFAFVFGDGLGFTTWSRSPSSAALLGDG
                     SLRVSASVENTGSRRGRQPVLVFVAPAGSPEALRFAGSTMVDLAPGTSTVAEVVVPSR
                     RLQRLLPSPEAGLIVSLGFSVRDREPARIEPRPVIFPPPPPV"
     assembly_gap    3357..3456
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3678..4733)
                     /locus_tag="PLK64_08915"
     CDS             complement(3678..4733)
                     /locus_tag="PLK64_08915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HPV79908.1"
                     /translation="MSTPAPALSVLDLVPVRSDQSTGDALVATRGLARVADELGFHRY
                     WLAEHHNMPAIAATNPPLLVAMVAAATEQIRVGSGGVMLPNHAPLVVAEQFALLEAAY
                     PGRIDLGIGRAPGTDQVTAWALRHGGGGVEDDAVARFPEAVANIIAMLSPEGVGLRLA
                     SGTHVLRATPRATSVPEVWLLGSSDYSAQLAAQQGLPYVFAHHFSGRGTAEALALYRD
                     NFQPSPDYPEPRTFLTVNAVVAPTREEAERLALPNLASMVALRTGQPLRAQQLVEEVE
                     ESNFSLDASDLARQMLDRWVVGTPESAEEQLCSLATMFAVDEVMVHPVAGAVRGTPTD
                     RSPAREQTLRLLRAALT"
     gene            4828..5331
                     /locus_tag="PLK64_08920"
     CDS             4828..5331
                     /locus_tag="PLK64_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79909.1"
                     /translation="MESVEQRLVTPFYLAMMGLNATEHAGDRWDDLVRVGRTATVTDV
                     KQLLAAGAWRPVVMGAWLSVAFSPKDLGPDLLLAVTRCQGSFTAPPLSVAAYLVLGAD
                     AGTALTNYVFRARDDERLGSATFVAAVVEALGGQPAVPPREEDRVDLAGMIGVAWRLR
                     TALTATS"
     gene            complement(5385..6089)
                     /locus_tag="PLK64_08925"
     CDS             complement(5385..6089)
                     /locus_tag="PLK64_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPV79910.1"
                     /translation="MPLASDTGIDLPGDAAIPNAEPPPSSYTYRWLAHLADRPLPRRE
                     VFERAITQMTFEPGEAVFDSGQDLPYLFMVLRGGIKVADPTRGNERLTGVAGVGDLVG
                     SLPSVLPAGMPDLLSARVAESTWEPSPQWGRTNRSAIAIVDTVVERMDMRVLMSLMRR
                     HAAWAMVGYHAMALHAMGQERRARDLLTLSAQERYLNFLVQAPELIGLLPQKDIAAYI
                     GVTPVGLSRIAARSRR"
     gene            complement(6249..6974)
                     /locus_tag="PLK64_08930"
     CDS             complement(6249..6974)
                     /locus_tag="PLK64_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPV79911.1"
                     /translation="MDVNAYALFQARAESPPSATRRFFELFGGGPLPDWEGLDEATES
                     ISADAGQVIFDRGTEHPYVYVVLDGSVKLLAIDERGNEHLVGLARAGELVASVGALAP
                     QGLARYVDEDLLGGNWSAESAMGTTDIRAVAIRACRLERVDFRVVHAKMREHGGTWAV
                     AVFNAALHYALVEEHRARQFLMLTAEDRYRHFLRRYPDLVGVLPQKDIGNYVGVTPVG
                     ISRIAARVRDEDRRRPIRTRAAQ"
     gene            7129..7899
                     /locus_tag="PLK64_08935"
     CDS             7129..7899
                     /locus_tag="PLK64_08935"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012802063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HPV79912.1"
                     /translation="MTSAPIAPEIPGAIIALARAARRITVLTGAGISAESGVPTFRDA
                     QTGLWTQVDPEDLATPAAWARDKPYVNAWFLWRVHLISAATPHAGHRALADWARRPDT
                     DLRIVTQNIDDLHERAGSEVLAHLHGSLFAWRCDRCERPAPTPQAPAQPRERIEPDTC
                     PHCAFGEIRPGVVWFNEALPERELADAIRVCRDADLVVVVGTSGIVQPAASLPHLAGA
                     RGIPVIEIDPRPTEFSPFATHVWRATAGAALPALVAAL"
     gene            complement(7960..8370)
                     /locus_tag="PLK64_08940"
     CDS             complement(7960..8370)
                     /locus_tag="PLK64_08940"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPV79913.1"
                     /translation="MSAPDLQAFLAREFPDIDRLGILVERVDERSITLRLPAESAMIR
                     PGGTISGPTMFSLADVTVWLLILAQIGPVALAVTTSVGMNFLRKPPPGDLVAEGELLK
                     LGRRLAIGHVRLHAEGSRDLVADASITYSIPPRG"
     gene            8451..>8559
                     /locus_tag="PLK64_08945"
     CDS             8451..>8559
                     /locus_tag="PLK64_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPV79914.1"
                     /translation="MPPRLTLQPLDESFERALVIVAHPDDIEYGTAAAVD"
BASE COUNT         1225 a         3009 c         2880 g         1345 t
ORIGIN      
        1 ccttcgacga cgctggcgtc atcaccggag gcgacccgtc gagccccttg cgcttcatcg
       61 tccccgtggc cgtcgccgtc gccacctcga tgatcctcgt cgtggccttg cgcccgatcg
      121 accggcacat ccagcgctcc caggtgccgc cctacagcgt gggcgagttc ttcgggtcct
      181 tcgtcttcaa cccccgcaag aaccccgact tcacctgggc ctgggtcagc cggttcatgt
      241 tcatgctcgg gatcgcctac ctgctcgtct accaggcccc ctactcctcc gcgcacctca
      301 agttcgaggg tgccgatctc gaccgggtcg tcctgtgggg caccgtcgtc accgtcgtgg
      361 gcacggtgct caccggctac gtcgccggcc agttgtccga ccggttcaag cgccgcaagc
      421 ctttcgtcat cggatcggcg atggggtatg ccgtgagcct ggtcttcctg tcgctcgccg
      481 caccggacaa ctccggtctg cccatggcgc tcgtcggcct gttcctcggc gggctggctc
      541 agggtgtcta cttcggcatc gacctcgccc tggtgaccga tgtcctgccc gaccgggagg
      601 ccgatgcggc caaggacctc ggggtgttca acatcgcctc ggccggcccg cagttcatcg
      661 caccgttcat cgccccggtg ttcctcggcc tgaacctgct cggctccggc gccaaccagg
      721 acaacttcgc cgcgctgttc gtcttcgccg cgatcttctc ggcgatcggt gccctgctcg
      781 tgctcccgat caaggccgtg cggtaggtcg acgatgtccg cacccgaagc cccatcgacg
      841 accctcgccg atccggcgcc gacccttgcc gaccggcgcc aacctggcgc cggcccggca
      901 acgactggga acaacggact gatcgacgaa ctgctcggcc ggctcaccct cgagcagaag
      961 gtcgagctga tcaccggcgc gagcttctgg acgacggccg cctgcccgga gatcgggctg
     1021 cgctcgctca ccctgtcgga cggtccggcg ggggtgcgcg gccggttctg gtccgagcag
     1081 cacccgtcgg ctgcgctgcc caacgcggtc tgcctcgcgg cgaccttcga ccccgagttg
     1141 gtcgagcggg ccggccgtct catcgggtcg gaagcgcggc gcaatggcgt cgacgtgctg
     1201 cttggcccga cggtcaacct gcaccgatcg ccccacggcg ggcgcaactt cgaggcgttc
     1261 agcgaggacg ccgtgctgac cgcacagacc ggtggcgctc tggttgacgg catccagagc
     1321 cagggtgtcg cagccaccgc caagcacttc atcggcaacg actccgagac cgagcggatg
     1381 acccttgatg cggtcatcga cgaggcgacg ctgcgcagca cctatgcgct gcccttcgag
     1441 gagctcgtcg tcgaccatga cgtggccgcg gtgatgagca gttacaacag catcaacggc
     1501 acctcgggta cggagtcgcc gctgctggaa tcgttgctgc gggcggagtg gggattcgcc
     1561 tcgctcgtca tctccgactg gggcgcggtt cgctcggtcg aggccgctgc cgcggcacgc
     1621 acggatctgg ccatgccggg gccggagtca ccgtggagtg agggcctggt tgcggcggtg
     1681 cgtgccggtc gcgccgacga ggccgacgtc gatgtgaagg tggcctccct gctgggtctg
     1741 gccgcacggc tcggtgcgct gagcggcata cctcctcggc aggacccgac cgcgctggac
     1801 gatccccacg acccgtcgtc cccggccgtc cgggatctcc tggcgacgat cgccgcccgc
     1861 ggcgccgtcc tgctgcgcaa cgagggcgcg accctgccgt tggagccgag tccggcgagc
     1921 atcgccgtca tcggccccgc ggccacgagg ctgcgcgtcg gcggcggggg cagcgcgacc
     1981 gtggtgccgc cccatgtcgt ctccgtcgtg acggggatcg aggcggcctt cccggacgcc
     2041 ctggtccacg ccgtcgaggg cgtcacccaa catcgcctgt tgcggccgat cggggatcga
     2101 tcgaccaacg acctgggggc agcgggatac gacgtggagc tcctcgacct ggaaggaacg
     2161 gtccttgccg ctcaggcgcg gactgggggc gagctcatct acggcatggg gttccccgat
     2221 ggagtcgacg gcgatctggt cactcgtatc cgggtgacca tgacgctgca cgagacgggg
     2281 ccgcgaagga tcggggtcgc cggaatcggg gacgccgaga tctggatcga aggtcagcgc
     2341 gttgccgcac agaccctgcg caacgagcac gacgatgaga ttgccgggct gtcgcgccca
     2401 ccgcagctcg tcttggatat ctcactcacc ggtggtgagc gcgtcgtcat cgagttcgct
     2461 cccgcccctg acaccatcgc cgcgctgcgg atcggcctcg aaggcccggc gcgatccacg
     2521 gcggacctgt tggaggaagc ggtggcggcg gcccgagccg cggacgtggc cgtggtcgtc
     2581 gtcggcacga ccgaggccga ggagtccgaa ggtttcgacc gggagagcct cagcctcggc
     2641 gccgacatcg acgccctggt cgaggccgtg gccgcggtga acccccgcac cgtcgtcgtc
     2701 ctcaacgtcg gcgcgcccgt cctgctccct tggcgggagc gggtggccgc gatcctgttg
     2761 gcccacttcc cggggcagga gggcggcacc gccatcggtc ggattcttgc gggcgccgag
     2821 gagccgggcg gtcggttgcc gagcagttgg ccgcgcaccg ccgactcccg ggttccgtcg
     2881 gtgacgccgg tggccggcac cctcgcctac caggagggtg aggcgttcgg ctaccgcgac
     2941 ccggagggcg acttcgcgtt cgtcttcggt gacgggctgg gcttcacgac ctggagccgg
     3001 tcgccctcca gcgcagcgct gttgggtgac gggtcactgc gggtcagcgc gtccgtggag
     3061 aacacgggct cgcggcgcgg gcgtcagccg gtcctcgtgt tcgtcgctcc cgccggctcg
     3121 ccggaggcgc tgcggttcgc ggggtcgacg atggtcgacc tggcaccggg gaccagcacg
     3181 gtggccgagg tcgtggtgcc cagccgccgg cttcagcgac tgctcccgag ccccgaggcg
     3241 ggcctgatcg tcagcctggg cttctcggtc cgcgaccgcg aacccgcccg gatagagccc
     3301 cgcccggtca tctttccgcc cccacccccg gtttaaggcc ggagatagcg ctcgcannnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngata gcgctcgcac ggtcatcttt
     3481 gggggctggg accgggtttg gggccggtga tagcgctcgc acggtcatgt gtgggggttt
     3541 ggcgcgggtt caaggccggt gatagcgctc gcacggtcat ctttgcgtgg gactgacgac
     3601 gccggtatgc cgcgcgcctg tccgcgagcg tgcggacggc cgcgcgcggc ataccggcga
     3661 cagacgtttg tgccccgtca ggtgagggcg gcgcgcagca ggcgaagggt ctgctcgcgg
     3721 gcgggagacc ggtcggtcgg cgtgccgcgc actgcccccg ccaccggatg aaccatcacc
     3781 tcgtcgacgg cgaacatcgt tgccagggag cacaattgct cctcggctga ctcgggtgtg
     3841 cccaccaccc agcggtccag catctgccga gccaggtcgc tggcgtcgag gctgaagttg
     3901 ctctcctcga cctcctcgac gagctgctgg gcccgcagcg gctgcccggt gcgcaacgcg
     3961 accatcgagg caaggttcgg cagagcgagg cgctcggcct cctcccgcgt gggcgccacg
     4021 accgcgttga cggtgaggaa ggtccgcggc tcggggtagt cgggtgaggg ctggaagttg
     4081 tcgcgataga gcgcgagcgc ctccgccgtg cctcgtccag agaagtggtg agcgaagacg
     4141 tagggcaggc cctgctgtgc tgcgagctgg gcggagtagt ccgacgatcc caacaaccac
     4201 acctcgggca ccgaggtcgc gcgtggcgtc gcgcggagca cgtgagttcc gctggccagc
     4261 cgaagcccga caccctccgg cgagagcatc gcgatgatgt tggcgacggc ctcagggaag
     4321 cgcgcaaccg cgtcgtcctc caccccaccg ccgccgtggc gcagggccca cgcggtgacc
     4381 tgatcggtgc ccggcgcccg gccgatcccg agatcgatcc gccctgggta tgccgcctcg
     4441 aggagggcga attgctcggc caccaccagc ggcgcgtggt tggggagcat caccccgccg
     4501 gatccgaccc ggatctgttc ggtcgccgcc gcaaccatcg ccacgagcag cggtgggttg
     4561 gtcgccgcga tcgccggcat gttgtggtgc tccgcgagcc agtagcggtg gaatccgagc
     4621 tcgtcagcca cgcgggccag accgcgggtg gcgacgagag cgtctcccgt gctctggtcg
     4681 cttcggacgg gcacgaggtc aaggactgac aaggcggggg ccggggtgct cacctcacct
     4741 gcagcagcaa cgtccccggt gctattccga gtcgggcaaa cgccctcctg cggccagcta
     4801 cccgtgaggc ggccgtatcg ttcaggtgtg gagtcggttg agcagcgctt ggtcacgccg
     4861 ttctacctgg caatgatggg cctcaacgcc accgagcacg ccggcgatcg gtgggatgac
     4921 ctggtccgag tgggacggac cgccaccgtg acggacgtca agcaactctt ggccgctggc
     4981 gcctggcggc ccgtcgtcat gggagcctgg ctcagcgtcg ccttctcccc caaggacctc
     5041 ggcccagatc tgctcctcgc cgtcactcgc tgccaagggt cattcaccgc gccgccgttg
     5101 tcggtcgcgg catacctggt gttgggagcc gacgcgggga cggccctgac caactacgtc
     5161 ttccgggcgc gcgacgatga gcggctcggg tcagcgacct tcgtcgccgc ggtggtcgag
     5221 gcgctggggg gccagccggc cgtccctcca cgcgaggagg accgcgtcga cctcgctggc
     5281 atgatcggcg tggcttggcg gctgcgaact gccctgacgg caacgtcctg acggcaacgt
     5341 cctgacggcg actcagcgac ccagcgactc agcgactcag cgactcagcg gcgcgagcgt
     5401 gcggcgatcc ggctcaaccc gaccggggtg accccgatgt aggcggcaat gtctttctgt
     5461 ggcaggagac cgatcaactc gggcgcctgg acgagaaagt tgagatagcg ctcctgtgcc
     5521 gagagggtca gcaggtcacg agcccgacgt tcctgcccca tcgcgtgcag cgccatggcg
     5581 tgatacccca ccatggccca ggccgcgtgc cggcgcatca acgacatcaa cacccgcatg
     5641 tccatccgct cgacgacggt gtcgacgatg gcgatggccg agcggttggt ccgaccccat
     5701 tgcgggctgg gttcccacgt gctctcggcg accctggcgg acaggaggtc gggcattccg
     5761 gcgggcagga cggaaggaag gctgcccacc aggtcaccga caccggccac ccccgtcagc
     5821 cgttcgtttc cccgcgtcgg gtcggcgacc ttgatccccc ctcggagcac catgaacagg
     5881 tacggcaggt cctggccgct gtcgaacacc gcctcgcccg gctcaaaagt catctgggtg
     5941 atggcccgtt cgaacacctc acggcggggc agggggcggt cggcgaggtg ggcgagccac
     6001 cgataggtgt agctggacgg aggcggttcg gcgttgggaa tcgccgcatc gcccggcagg
     6061 tcaataccgg tgtctgatgc caacggcata cgcgctcctt gcaggttctc tccccgagtc
     6121 cctcccctcc atatgcacca taccgatcgt tgcgcgttct acgaatgcca ggggtggcct
     6181 caagacaaag attcagccgt cgatgccgac gttctgtccc cttcgcgtgc tctgcgtgtt
     6241 ttgtgacgtc actgagcggc tcgcgtgcgg atcggccgtc gacgatcctc gtcgcggacg
     6301 cgcgcggcga tccgactgat cccgaccggt gtgacgccga catagttgcc gatgtccttc
     6361 tggggaagca ccccgaccag gtcggggtag cgccgcaaga agtgccgata gcgatcctca
     6421 gccgtcagca tgaggaactg gcgggcccgg tgttcctcga cgagcgcgta gtgcagcgcc
     6481 gcgttgaaga cggcaacggc ccacgtcccc ccgtgctcac gcatcttcgc gtggaccacc
     6541 cggaagtcga cccgctcaag gcggcaggcg cgaatggcga cggcccggat atcggtggtt
     6601 cccatagcgc tctcagcgct ccagttcccg ccgagcaggt cctcatcgac gtaccgtgcc
     6661 aagccttggg gtgccagggc ccccacgctg gcgaccaact cgccggctcg ggccagaccc
     6721 acgaggtgct cgttcccacg ctcatcgatc gcgagcaact tcaccgagcc atccagcacg
     6781 acgtagacgt aggggtgctc cgtgccacgg tcgaagatca cctgtcccgc gtcggcgctg
     6841 atcgactcag tggcctcgtc caggccctcc caatcgggca gcgggccacc accgaacaac
     6901 tcgaagaacc gtcgggtggc cgacggcggc gactcggccc gggcctggaa cagcgcgtag
     6961 gcgttgacgt ccatggtcgc tcctgggcag tacacggcac gtcaacgcgg tcggcgcgcc
     7021 ttcggtcccg ttccgcttcg cggtcattat cgcagcacgg tttgactccc ggggggtttt
     7081 gccgacactc tcgtcgatgc tatttcgggc gctggcatga tggcatttat gacctcggcc
     7141 cctatcgcgc cagagattcc cggcgcgatc atcgccctcg cccgagcggc ccggcgcatc
     7201 accgtcctca ccggtgcggg gatttccgcc gaatccggtg tgccgacctt ccgcgacgca
     7261 cagaccggcc tgtggaccca ggtcgacccc gaggatctgg ccactccggc cgcctgggcc
     7321 cgcgacaagc cctacgtcaa cgcctggttc ctctggcggg tgcacctcat ctcggcggcc
     7381 accccgcacg ccggccatcg tgcgctggca gactgggccc gccggccgga cacggacctg
     7441 cgcatcgtca cccaaaacat cgatgacctg cacgaacggg ccggttccga ggtcctcgcg
     7501 cacctgcacg ggagcctctt cgcctggcgc tgcgaccgct gcgagcgccc cgcgccgacg
     7561 ccgcaggccc ccgcgcagcc ccgcgagcgg atcgagccgg acacgtgccc gcactgcgcc
     7621 ttcggggaga tccgcccggg agtggtgtgg ttcaacgagg cgcttcccga gcgcgagctc
     7681 gcggacgcca ttcgggtatg ccgtgacgcc gacctggtcg tcgttgtcgg cacctcgggc
     7741 atcgtccaac cagcagcgag ccttccgcac ctggctgggg cacgcggcat accggtcatc
     7801 gagatcgacc cgcgccccac ggagttcagc ccgttcgcga cccacgtgtg gcgtgcgacg
     7861 gccggggcgg cgttacccgc cctcgtcgcg gccctctgac cccggctcgc gaacccggct
     7921 cgcgaaccgg gctcgcgaac ccggctcagc caaccccgct cagccgcgcg gcgggatcga
     7981 gtacgtgatc gacgcgtcgg ccacgaggtc gcggctgccc tcggcatgca accgcacgtg
     8041 cccgatcgcc aggcgccgac cgagcttgag cagttccccc tcggccacga gatcaccggg
     8101 cgggggtttg cgcaggaagt tcatcccgac acttgtcgtc accgcgaggg cgaccggccc
     8161 gatctgggcg aggatgagca gccacacggt gacatcggcg agcgagaaca tcgtcgggcc
     8221 cgagatcgtc ccgccgggcc ggatcatcgc cgactccgct ggcagtcgca gcgtgatgga
     8281 ccgttcgtcc acccgctcga caaggatgcc caggcgatcg atgtcgggaa actcgcgcgc
     8341 caggaacgcc tgcagatcgg gtgcgctcat cgcggtcacg acctcaagtt aatcaccgcc
     8401 atcgggggcc cgaagcgggc tatcgtccgt ccatgagcga gccctccggt atgccgcccc
     8461 gcctcaccct gcaaccgctc gacgagagct tcgagcgcgc cctcgtcatc gtggcccacc
     8521 cggacgacat cgaatacggc acggccgccg ccgtcgaca
//
LOCUS       DAOMZY010000136         8548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_7802_length_8548_cov_8.358884, whole genome shotgun sequence.
ACCESSION   DAOMZY010000136 DAOMZY010000000
VERSION     DAOMZY010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 8548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8548
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7802_length_8548_cov_8.358884"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /gene="hutI"
                     /locus_tag="PLK64_08950"
     CDS             complement(<1..1025)
                     /gene="hutI"
                     /locus_tag="PLK64_08950"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HPV79915.1"
                     /translation="MTATLITGIAELVTNDSSWSAAGQAPDVARLGIVTDAAVLVEAG
                     EVVWVGPASAAPQADSRVDVGGCAVMPGFVDSHTHLVFAGDRAAEFAARMVGTAYDGG
                     GIATTVAATRGATDDELRTLLAARIAELRRQGTTTVEIKSGYGLSVEDELRLLRLARE
                     VTPHVTFLGAHVVPAEYAADRAAYLDLVTGPMLAAVVAQGAATYVDVFCEPLSAHAFT
                     EDEARVILRAGRAAGLAVRVHGNQLGPGPGVRLACELGAVSVDHCTYLSDADVAALAE
                     TGTVATLLPGVEFSTRHPYPDARRLLDAGVAVALATDCNPGTCYTSSMPFVIALAVRE
                     LRMTPAEA"
     gene            complement(1022..2353)
                     /locus_tag="PLK64_08955"
     CDS             complement(1022..2353)
                     /locus_tag="PLK64_08955"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HPV79916.1"
                     /translation="MTSYWCEYAWLPGGLAASVRLEMMGERIVGVSADVEARPEDVRL
                     AGVTLPGLANTHSHAFHRALRGRTHGDGGTFWTWRERMYAVASRLDPARYLALARAAY
                     AEMALAGFTAVGEFHYVHHQPDGTPYADPNAMGAALIQAAAEAGIRLTLLDTIYLAGG
                     LGPDGHLPLDVVQQRFSDDSVDAWSTRVGLMSDTPTARMGAAIHSVRAVPKDALAGVV
                     AASVGRPLHVHLSEQPAENATALAFYGVTPTALLEGAGALGPMTTAVHATHLTDDDIA
                     SLASAGVTACFCPTTERDLADGIGPARALADAGVPLALGSDQHAVVDPFEEARGLESH
                     ERLVTLERGRFTPGQLTDALSRNGYAALGWPDGGRLAAEALADFVTVRLDSPRTAGAA
                     PEQILYAATGADVTTVVVGGEVVVSAGQHRLGDVGRLLTDAISALDDGAAS"
     gene            complement(2358..3617)
                     /locus_tag="PLK64_08960"
     CDS             complement(2358..3617)
                     /locus_tag="PLK64_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HPV79917.1"
                     /translation="MAPFSTPDGAAARSFDRMWADLAPIGRNPATGGYERFAWTREDH
                     TLREWFAGECGARGLEVVRDRAGNQWAWWGDPDARPGIVLGSHLDSVADGGAFDGPLG
                     VVSALAALDALRDNGFTPRMPLGIANFADEEGGRFGVACAGSRILTGALSPDRARSLT
                     DGDGVTMAQAWTAAGRDPATLGPDPDALRRVAAYVEVHVEQGRALCVDPDDPFGDPAL
                     WDPARAVAIGTEIWPHGRWQIDVFGEANHAGTTALADRNDAMLGFASVVASARMLARE
                     RGCVATVGKVIARPNAVNAIADHVTTWLDARGADPEAVRGLVQTLRDEVSEDGTTVDE
                     VSWTPATVFDTRLRDRLAAVLPEAPLLATGAGHDAGILANAGIPTAMLFVRNPTGVSH
                     SPQEFAERDDCIRGIHALAAAAAELAG"
     gene            complement(3749..5404)
                     /locus_tag="PLK64_08965"
     CDS             complement(3749..5404)
                     /locus_tag="PLK64_08965"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HPV79918.1"
                     /translation="MEGARPVRAPRGTTLTARSWQTEAPLRMLMNNLDPENAERPDDL
                     VVYGGTGRAARDWRSFDAMVRTLTTLAPDETMLVQSGKPVGVFQTHEWAPRVLIANSN
                     LVGDWATWPEFRRLEHLGLTMYGQMTAGSWIYIGTQGIVQGTYETFAAVAEKRFNGTL
                     AGTLTLTGGCGGMGGAQPLAVTMNEGVCLVVDVDPARLHRRVDHRYLDEVAHDLDDAV
                     EKVLRAKAERRAWSVGVVGNAAEIFPELLRRGVPIDIVTDQTSAHDPLSYLPEGVALE
                     EWAELAEKKPEEFTDRARASMAKQVKAMVEFQDAGAEVFDYGNSIRDEARQGGYERAF
                     EFPGFVPAYIRPLFCEGKGPFRWAALSGDPKDIAVTDKAVLDLFPDNDRLHKWIRGAQ
                     ERIAFQGLPARICWLGQGERDKAGLAFNDLVRTGAVQAPIVIGRDHLDTGSVASPYRE
                     TESMLDGSDAIADWPLLNALTAASSGATWVSIHHGGGVGIGRSIHAGQVSVADGTPLA
                     AQKLARVLTNDPGMGVIRHVDAGYEAADVVAADLGVRIPMREQ"
     gene            5459..5716
                     /locus_tag="PLK64_08970"
     CDS             5459..5716
                     /locus_tag="PLK64_08970"
                     /inference="COORDINATES: protein motif:HMM:NF019324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HPV79919.1"
                     /translation="MSLNIKNERVCRLAKEAAALTGQTQVRVLEQALERFIEERSKAA
                     EAQERFRRVQEILASYTVTDEERAATKRFMEEMYDENGLPV"
     gene            5713..6141
                     /locus_tag="PLK64_08975"
     CDS             5713..6141
                     /locus_tag="PLK64_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPV79920.1"
                     /translation="MILDSSAVCAIVLKEAEALAYSNAASEAESVRMAAPTFVEAAMV
                     IDGKGRPELTRSFDEVLDWLHVEIAPFTERQARLAREAFRDFGRGSGHRARLNLGDCF
                     AYALAKDVGEPLLYKGDDFVHTDVRAVFPRGYVEGVSPRP"
     gene            complement(6178..7731)
                     /gene="hutH"
                     /locus_tag="PLK64_08980"
     CDS             complement(6178..7731)
                     /gene="hutH"
                     /locus_tag="PLK64_08980"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HPV79921.1"
                     /translation="MSTPDHPAVTVGDGPLTIDHVVAIARHDARVVISAAAVARMAQT
                     RAVIDGLADDVEPHYGVSTGFGALATRHIPLELRTQLQRSLVRSHAAGSGAEVEREVV
                     RGLMVLRLATLATGRTGIRPVVAQTYAAMLNAGITPVVHEFGSLGCSGDLAPLAHCAL
                     AAMGEGPVRLADGTPSDAASALQDNGIEPVVLREKEGLALINGTDGMLGMLCLAISDL
                     RGLLTVADVTTAMSVEGLLGTDDVFAADLQALRPQPGQALSAANIRRVIADSPIRHSH
                     DTEDCPRVQDAYSLRCAPQVTGAARDTVEHAARIAAYELASAVDNPVVTLDGRVESNG
                     NFHGAPVAYVLDFLAIVAADVASMSERRTDRFLDRSRSNGLPAFLAGDPGVDSGHMIA
                     QYTQAAIVSEMKRLAAPASVDSIPSSAMQEDHVSMGWSAARKLRRSVDGLTRVLAIEL
                     LTAARGLDLRAPLQPSPATGAVVAALRERVAGVGPDRFLAPEIEYAVQLVASGRALAA
                     AESVTGPLA"
     gene            7832..>8548
                     /locus_tag="PLK64_08985"
     CDS             7832..>8548
                     /locus_tag="PLK64_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPV79922.1"
                     /translation="MDPNPAVAHALDVLQLLARHTEPLPAAAIARDLGLPRSSTYRIL
                     MTLADRGFVSHLRESGRFGLGAAAYELGSAYRRQAPLQRIAAPLLARLVDATTHNAHL
                     AVMHGRDVLYVVEERAAGRPRLVTDVGVRLPAELTASGLAMLSRLPAAQVRALYPAAS
                     QLIRREEHGPRTLTELRRALTQTRARGYAVESGTVTEGLASVAVAVVDHTDHPVAAVA
                     VTYPSDVVDTAGRDRLVEQVR"
BASE COUNT         1266 a         2999 c         3081 g         1202 t
ORIGIN      
        1 gcctctgcgg gggtcatccg caactcccgc acggcgagcg cgatgacgaa cggcatcgat
       61 gaggtgtagc aggtgcccgg gttgcagtcg gtcgccagcg ccacagcaac accggcgtca
      121 agcagccggc gcgcgtcggg ataggggtgc cgggtcgaga actcgacccc cggcaacagc
      181 gttgcgacag ttccggtttc ggcgagtgca gcgacgtcgg cgtcggacag gtaggtgcag
      241 tggtcgacgc tcaccgcccc gagctcgcag gccaatcgca cgcccggccc ggggcccagt
      301 tggttgccat gcacgcgcac ggccaggccg gcggcgcgac cggcccgcag gatcacccgg
      361 gcctcgtcct cggtgaaggc gtgcgccgac agcggctcgc agaacacatc gacatacgtg
      421 gccgcaccct gggcgacgac cgccgccagc atcggcccgg tcaccaggtc gaggtatgcc
      481 gcgcggtcgg cggcatactc ggccggcacg acgtgcgctc cgaggaaggt gacgtgcggc
      541 gtgacctcgc gggccaggcg cagcagtcga agctcgtcct cgacgctcag cccgtaacca
      601 ctcttgatct cgacggtcgt cgtgccctga cgccgcaact cggcgatccg cgcggccaac
      661 agcgtgcgca actcgtcgtc ggtcgccccg cgcgtcgccg ccacagtcgt cgcgatccca
      721 cccccgtcgt atgccgtgcc caccatccgg gccgcgaact cggccgcccg gtcaccggca
      781 aagacgaggt gagtgtgcga gtcgacgaag ccgggcatga cggcgcaccc gcccacgtcg
      841 acgcgcgaat ctgcctgcgg ggcagcgctg gccgggccta cccagacaac ctccccggcc
      901 tcgacgagga cggccgcgtc ggtgacgatc cccaaccgag caacgtcggg cgcctggccc
      961 gcagccgacc agctcgaatc gttggtgacg agctcggcga tccccgtgat gagggttgcg
     1021 gtcatgaagc tgctccgtcg tcgagggccg agatggcgtc cgtcaagagt cggccgacgt
     1081 cgcccagtcg gtgttgtccg gccgagacga ccacttcacc gccgacgacg accgtggtga
     1141 cgtcggcgcc ggtggcggca tagaggatct gctcgggggc ggcaccggcg gtgcgcggcg
     1201 agtccagtcg cacggtgacg aagtcggcca gcgcctccgc ggccagccgg cccccgtcgg
     1261 gccagccgag ggcggcatac ccgttgcggg acaacgcatc cgtgagttgg ccgggggtga
     1321 agcgcccgcg ctcaagagtg acgagccgct catgcgactc caaaccgcgg gcctcctcga
     1381 acgggtcgac caccgcgtgt tggtcggaac cgagggcgag cggcacaccc gcgtcggcca
     1441 aggcgcgagc tggcccgatg ccgtcggcga ggtcacgctc ggtcgtgggg cagaagcatg
     1501 cggtcacgcc ggcgctggcc agcgaggcga tgtcgtcgtc ggtgaggtgg gtcgcatgga
     1561 ccgccgtggt catgggcccg agcgccccag ccccctccag gagcgccgtc ggcgtcacgc
     1621 catagaaggc cagcgcggtc gcgttctcgg cgggctgctc ggagaggtgg acgtgcaggg
     1681 ggcgccccac tgacgccgcc acgactcccg ccagcgcgtc cttcgggacg gcacgaaccg
     1741 agtggatcgc cgcccccatg cgtgccgtcg gggtgtcgct catgagccca acccgcgtcg
     1801 accacgcgtc gaccgagtcg tcggagaacc gttgctgcac aacgtcaagc ggcagatggc
     1861 catcggggcc gaggccgccg gcgagataga tcgtgtcgag cagggtgagc cggatgccgg
     1921 cctcagcggc cgcctggatg agcgccgccc ccatggcgtt cgggtcggcg tagggggtgc
     1981 cgtcgggttg gtggtgcaca tagtggaact ccccgaccgc ggtgaatccc gccagcgcca
     2041 tctccgcgta tgccgcccgc gccagcgcca ggtaacgcgc cgggtccagg cggctcgcga
     2101 cggcatacat gcgctcgcgc caggtccaga aggtgccgcc gtccccatgg gtccgaccgc
     2161 gcagggcacg gtggaacgcg tgggagtgcg tgttggcgag ccccggcagg gtcacccctg
     2221 cgagtcggac atcctcgggc ctcgcctcga catccgccga cacgccgacg atccgctcgc
     2281 ccatcatctc gaggcggacg ctagcggcca atccgcctgg caaccaggcg tattcgcacc
     2341 agtaggaggt cacgtggtca cccagccaat tcggcagcag cggcggccaa ggcgtggata
     2401 ccccggatgc agtcgtcccg ctcggcgaac tcctggggcg agtgcgagac gccggtcggg
     2461 ttgcgcacga acagcatcgc cgtggggatc ccggcattgg cgaggatgcc tgcgtcgtgg
     2521 cccgcgccgg tggcgagtag gggagcctcc ggcaacaccg cggccagacg gtcgcggagt
     2581 ctggtgtcga aaaccgttgc gggagtccag gacacctcgt ccacagtcgt tccgtcttcg
     2641 gacacctcat cgcgcagggt ctggacgagc ccgcgcacgg cctccgggtc ggcccctcgt
     2701 gcgtcgagcc aggtcgtgac gtggtcggcg atcgcattga cggcattggg ccgggcgatg
     2761 accttgccga ccgtggccac gcacccacgt tcgcgggcca gcatccgcgc cgacgccacg
     2821 accgacgcga agccgagcat cgcgtcgttg cggtccgcca gcgccgtcgt ccccgcatgg
     2881 ttggcctcgc cgaagacatc gatctgccac cggccgtggg gccagatctc ggtgccgatc
     2941 gcgaccgccc gagcggggtc ccacagggcc gggtcgccga aggggtcgtc cgggtcgacg
     3001 cacagagccc ggccctgctc gacatgcacc tcgacgtagg ccgccacacg gcgcagggcg
     3061 tccgggtccg gccccagcgt ggccgggtcc ctacccgcgg cggtccaggc ctgcgccatc
     3121 gtcaccccgt caccatcggt caggctgcgg gcccggtccg gtgacaaggc cccggtgagg
     3181 atccgcgacc cggcgcaggc gacgccgaac cgtcccccct cctcgtcggc gaagttggcg
     3241 atgccgagcg gcatacgagg ggtgaagccg ttgtcccgca acgcatcgag cgcggccagc
     3301 gcgctgacga cgccgagcgg gccatcgaag gcgccgccgt cggcgaccga gtcgagatgg
     3361 gagccgagca cgatgccggg tcgcgcgtcg gggtcccccc accaggccca ctggttgccg
     3421 gcacggtccc gcacgacctc aagcccgcgc gccccgcact ctccggcgaa ccattcccgc
     3481 aaggtgtggt cctcacgggt ccaggcgaac cgctcgtaac cccccgtcgc ggggttgcga
     3541 ccgatgggcg cgaggtcggc ccacatccgg tcgaacgacc gggcggctgc gccgtctggc
     3601 gtgctgaagg gggccatggg gtctcctttt cgggctcagt cgcgcgcttg tatgaggttg
     3661 gtgtcacttg tatgagtgtc tagcctcatc caagcgtcac taccctcata caagctgcgc
     3721 accaccgagg tcggcgcggg cggcacactc actgctcgcg catggggatg cggacgccga
     3781 ggtcggccgc gacgacgtcc gcagcctcgt agcccgcgtc gacgtgccgg atgacgccca
     3841 tgcccgggtc gttggtgagc acgcgggcga gcttctgcgc ggccagcggt gtcccgtcgg
     3901 cgaccgacac ctgacccgcg tggatggatc gaccgatgcc gacgccgccg ccgtggtgga
     3961 tcgacaccca cgtggcgccc gacgaggcgg ccgtcagcgc gttgagcagc ggccagtcgg
     4021 cgatcgcgtc ggacccgtcg agcatcgact cggtctcgcg gtaggggctg gccaccgagc
     4081 cggtgtcgag gtggtcgcgg ccgatgacga tcggggcctg gaccgcaccg gtgcgcacga
     4141 ggtcgttgaa cgccaacccg gccttgtcgc gctcaccctg gccgagccag cagatccgcg
     4201 ccggcaggcc ctggaaggcg atgcgctcct gcgcgccgcg gatccacttg tgcaggcggt
     4261 cgttgtcggg gaacaggtcg agcacggcct tgtcggtgac ggcgatgtcc ttggggtcac
     4321 ccgagagggc ggcccagcgg aacgggccct tgccctcgca gaacagcggc cggatgtagg
     4381 ccggcacgaa gccggggaac tcgaacgccc gctcgtagcc gccctgacgg gcctcgtcac
     4441 ggatcgagtt gccgtagtcg aagacctcgg cgccggcgtc ctggaactcg accatcgcct
     4501 tgacctgctt ggccatcgag gcccgggcgc gatcggtgaa ctcctcgggc ttcttctcgg
     4561 cgagctcggc ccactcctcc agcgccacac cctcgggcag gtaggacagc gggtcgtggg
     4621 cgctggtctg gtcggtgacg atgtcgatcg gcacgccgcg gcgcagcagc tcggggaaga
     4681 tctcggcagc gttgccgacg accccgaccg accaggcccg acgctcggcc ttggcgcgca
     4741 gcaccttctc gacggcgtcg tcgaggtcgt gggcgacctc atcgaggtag cggtggtcga
     4801 cgcggcggtg caggcgggcg gggtcgacgt cgaccacgag gcagacgccc tcgttcatcg
     4861 tgaccgcgag cggctgggcg ccacccatgc cgccgcagcc gccggtgagg gtcagggtgc
     4921 cggccagggt gccgttgaac cgcttctcgg ccaccgcggc gaaggtctcg taggtgccct
     4981 ggacgatgcc ctgggtgccg atgtagatcc acgacccggc ggtcatctgg ccgtacatcg
     5041 tgaggccgag gtgctcgagg cggcggaact cgggccaggt cgcccagtcg ccgacgaggt
     5101 tggagttggc gatgagcacg cgcggcgccc actcgtgggt ctggaagacg ccgaccggct
     5161 tgcccgactg gacgagcatc gtctcgtcgg gcgcgagcgt cgtcagcgtg cgcaccatcg
     5221 cgtcgaaact gcgccagtcg cgcgccgccc gcccggtgcc gccatagacg accaggtcgt
     5281 cgggccgctc ggcgttctcg gggtcgaggt tgttcatgag catccgcaac ggagcctcgg
     5341 tctgccacga acgggcagtg agggtggtgc cgcggggggc acggacgggg cgggcgcctt
     5401 ccatggcgga ctccttcagg gtagattggt agacagtatg ggtagaaagg tgtaaccggt
     5461 gagtctcaac atcaagaacg agcgggtatg ccgcctggcc aaggaggcgg ccgcgctcac
     5521 cgggcagacc caggtgcgcg tgctcgagca agccttggag cggttcatcg aggagcgcag
     5581 caaggctgcc gaggcgcagg agcggttccg tcgggtccag gagatcttgg cctcatacac
     5641 ggtcaccgac gaggagcgag cggccacgaa acggttcatg gaggagatgt acgacgagaa
     5701 cgggctcccg gtgtgatcct cgacagttcg gcggtgtgcg ccatcgtgct gaaggaggcc
     5761 gaagcattgg cctactccaa cgccgcgagc gaagcggagt cggtgcgcat ggcggcaccc
     5821 accttcgttg aggccgccat ggtgatcgac ggcaagggcc gaccggagtt gacgaggtcg
     5881 ttcgatgagg tcctggactg gctgcacgtc gagatcgcgc cgttcaccga gcgtcaggcg
     5941 cgcctcgccc gcgaggcgtt ccgcgacttc ggccgcggct cgggccatcg ggcccggctc
     6001 aaccttggcg actgcttcgc ctacgccctg gccaaggacg tcggcgagcc gctcctctac
     6061 aagggcgacg actttgtcca caccgacgtc cgcgcggtgt tcccccgggg ttatgtcgaa
     6121 ggcgtgtcgc cccgcccctg atgggctccg gacgctgctc ggcctcatca gcacacctca
     6181 ggccaacggc ccggtgacgg actcggccgc cgcgagggcg cggcccgacg cgacgagctg
     6241 cacggcatac tcgatctcgg gtgccaggaa gcggtcagga cccaccccgg caacccgttc
     6301 gcgcagggcg gcgacgaccg cgccagtggc ggggctcggc tgcagcggcg ctcgcaggtc
     6361 caacccccgt gccgcagtga gcaactcgat ggccagcacg cgggtgagcc cgtcgaccga
     6421 gcgccgcagt ttgcgggcag ccgaccagcc catcgagacg tggtcctcct gcatcgccga
     6481 cgacgggatc gagtcgacgc tcgcgggcgc ggccagccgc ttcatctccg agacgatcgc
     6541 ggcctgggtg tattgcgcga tcatgtggcc gctgtcgaca cccgggtcgc ccgcgaggaa
     6601 ggccggcagt ccgttgctgc gggagcggtc caggaagcgg tcggtgcgcc gctcgctcat
     6661 gctcgcgacg tcggccgcga cgatcgcgag gaagtccagg acgtaggcga ccggcgcccc
     6721 gtggaagttg ccgttgctct ccacgcgccc gtcgagcgtc accacgggat tgtcgacggc
     6781 agaggcgagt tcgtatgccg cgatccgcgc cgcatgctcg accgtgtccc gcgccgcccc
     6841 cgtcacctgg ggggcgcacc gtagcgagta ggcgtcctgg acgcgggggc agtcctcggt
     6901 atcgtgcgag tgccggatcg ggctgtcggc gataacccgg cgaatgttcg cggccgacaa
     6961 ggcctggccc ggctgcggcc gcagggcctg aaggtcggcg gcgaagacgt cgtcggtgcc
     7021 gagcaggccc tcgacgctca tcgcggtcgt cacgtcggcc acggtgagca gcccgcgcag
     7081 gtccgagatc gccaggcaca gcatgccgag cattccgtcg gtgccgttga tgagggccag
     7141 gccttccttc tctcgcagga ccacaggctc aatgccgttg tcctgcaggg cgctcgcggc
     7201 gtccgaggga gttccgtcgg ccagccggac cggcccttcg cccatggcgg ccagcgcgca
     7261 gtgggccagc ggagccaggt cgcctgagca cccgagtgag ccgaattcat gcacgacggg
     7321 cgtgatcccg gcgttgagca tcgcggcata cgtctgcgcg acgacggggc ggatccccgt
     7381 gcggccggtg gcgagggtgg ccaggcgcag caccatgagc ccgcggacga cctccctctc
     7441 gacctcggca ccgctgccgg ccgcgtgcga gcgcacgaga gagcgctgca gttgggtgcg
     7501 caactcgagg gggatgtgcc gggtggccag ggcgccgaag ccggtcgaga ctccgtagtg
     7561 cggctcgaca tcgtcggcca gcccgtcgat gaccgcccgg gtctgggcca tccgggccac
     7621 ggcggcggca gagatgacga cgcgggcgtc atgccgagcg atggcgacga catggtcgat
     7681 cgtcaacggt ccgtcgccga cggtcaccgc ggggtggtct ggagtgctca tgtgcccatt
     7741 agagggtgcg gtgtcgcctt ctgggagagg ccgacaccag atcgtgtccc agatatgaga
     7801 caccctggtc aagccgtgag agcctgaccg catggatccg aacccggccg tcgcgcacgc
     7861 cctcgacgtg ctgcaactgc tggcacggca taccgagccg ctgccggccg ccgcgatcgc
     7921 ccgcgacctg ggcctgcccc ggtcgagcac ctaccgcatc ctcatgaccc tcgcggaccg
     7981 cggcttcgtc agccacctgc gcgagtcggg ccgtttcggg ctgggtgccg cggcctacga
     8041 gttgggctcc gcctatcggc ggcaggcgcc gctgcagcgg atcgccgcgc cgctgctcgc
     8101 ccgactggtc gatgcgacga cgcacaatgc gcacctcgcc gtcatgcacg gccgggacgt
     8161 cctctacgtc gtcgaggagc gcgccgccgg acgtccgcga ctggtcaccg acgtcggggt
     8221 gcgactgccc gccgagctca ccgcgagcgg cctcgcgatg ctgtcgagac tccccgccgc
     8281 acaggtgcgc gcgctgtatc ccgctgcgtc ccaactgatc cggcgcgagg agcacggccc
     8341 acgcaccctc accgagctgc gccgcgcgct gacgcaaacc cgcgccaggg ggtatgccgt
     8401 ggagtccggc accgtgaccg agggcttggc gtcggtggcc gtcgccgtcg tcgaccacac
     8461 cgaccatccg gtggccgcgg tcgccgtgac ctacccgtcc gacgtcgtcg acaccgcagg
     8521 ccgagaccgg ttggtcgagc aggtgcgc
//
LOCUS       DAOMZY010000137         8362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_8024_length_8362_cov_5.483688, whole genome shotgun sequence.
ACCESSION   DAOMZY010000137 DAOMZY010000000
VERSION     DAOMZY010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 8362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8362
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8024_length_8362_cov_5.483688"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1437)
                     /locus_tag="PLK64_08990"
     CDS             complement(<1..1437)
                     /locus_tag="PLK64_08990"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPV79923.1"
                     /translation="MTPLVPQETEPTFADFGVHPDIVASLSDLGIIHPFPIQAMTLPV
                     ALGGHDIIGQAKTGTGKTLGFGVPMIQRVVTPADEGWADLAKPGKPQALAVAPTRELA
                     VQVSSDLERAGKQRGVRVLTVYGGRAYEPQVEALKRGVEIVVGTPGRLIDLAKQGHLD
                     LSQARIVVLDEADEMLDLGFLPDVETLMSLTPAGRQTMLFSATMPGAIVALARRYMTQ
                     PTHIRAMGDETENAHTVKAIEQFIYRAHSLDKVELLSRVLQARDRGLVLIFTRTKRAA
                     ARVTDELVERGFAAAAIHGDLGQGAREQALRAFRHGNVDVLVATDVAARGIDVEHITH
                     VVNYQCPEDEKTYVHRIGRTGRAGNTGIAITLVDWEDMPRWSMIDRQLDLGIPEPVET
                     YSSSEHLYAELDIPSDVSGYLPNAKRTRAGLDAEELEDLGETGRSGGHRGPGGGGRDG
                     GRGGRDGGGRDGGRGGRDSGGRGGRGGRD"
     gene            complement(1651..3372)
                     /locus_tag="PLK64_08995"
     CDS             complement(1651..3372)
                     /locus_tag="PLK64_08995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPV79924.1"
                     /translation="MTGVGRHTHDMPDAAAFGAWVAPHLPALSRYAHRLVPAAAADDV
                     VQEALVRAWSRRATYDPARGSAAPWLLAIVADQARRHRTRGPRPTTLLADDALTAGPT
                     EPDVDLERAVARLPHRQRQAVDLYYFVGLDIASVAQTMGCAPGTVQATLPRPERDCAS
                     CWETSMTDRPDPIDEQLQGYAARWRDALPPPPAVPTDLPTHSAGFGRRGRGVAGWLPL
                     VAAAAVLAVVVVAVRLGQGGTDLGSVGGPTASATTTATSSATATGSAVNNGVVPWVPL
                     PATNPLIPVTTVPASPDPALAQGLPVCRAADLTATSDIEGATGTMYLFVQIVSRTGTA
                     ACRLEGFPTVVSLVNGAAVSIPSVHGTVVSDAPGGIYPHPVLVAPDVPAALTLFWSNW
                     CGQAVRQTHVRLTWPSSAGGVTVPGFGQSPGCQMEPGTVVKTPFGVSTFKPAEVRDEL
                     VTTAYAGVTATMDAPSVVAVGEPIRLTVTLTAPRDIPLEPCPDILVGDYTQPPDGTND
                     RYALNCAAVPYRDSAGRPYLPATVPVRFAAEVPAANVGEGTKLLWRLALPDGADPVDA
                     GSATEVR"
     gene            complement(3763..4203)
                     /locus_tag="PLK64_09000"
     CDS             complement(3763..4203)
                     /locus_tag="PLK64_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79925.1"
                     /translation="MSTAAPTAPTGTFGDHVAESHLAASASSGTAKKVELFARGASIG
                     WLGENGGQWCVVTGSDAALSLESYLYGGILYFRNAADTSRYLSVSDGYHYVGFYNWIG
                     ATGWVLNGNVLTSLYTNAQLSYWSTDNGYLYANSGYTPLTVVFH"
     gene            4459..5151
                     /locus_tag="PLK64_09005"
     CDS             4459..5151
                     /locus_tag="PLK64_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like fold-containing protein"
                     /protein_id="HPV79926.1"
                     /translation="MTDDTGVAGSAAVGEVPHEAVVDLLGALSYAQLSGFEALAADAA
                     MTPHLRAKLAFARMGVEMFHRCEQVGQRLRELGADHESAMAPFVTSVDAFHERTKPSD
                     WLEGVVKAYVGDGIVRDFYSEMATHLDPANEAFVAQMLGQAGGAEAIVHEVREAIAGD
                     ARRSGRLALWARRIMGEAIHATQSVAVERDSLTALLVGGEPGSDLNEVGRMFARISAA
                     HTVRMEALGLSA"
     gene            complement(5272..5526)
                     /locus_tag="PLK64_09010"
     CDS             complement(5272..5526)
                     /locus_tag="PLK64_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HPV79927.1"
                     /translation="MSILGAIVGGLIVGLLARFILPGRQGLSLPITAAIGIVAALIGW
                     WVAGKLGVQTTSGIDWLRWIISTIIAAVGIVIYGKVTSKA"
     gene            complement(5704..5928)
                     /locus_tag="PLK64_09015"
     CDS             complement(5704..5928)
                     /locus_tag="PLK64_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HPV79928.1"
                     /translation="MEVRIGVRDVAREIAFETNETPAAIGAAVAVALRDGSVLDLADD
                     KGRRYFIPAAVIGFVHVGEAEKGKVGFGAH"
     gene            complement(6062..6787)
                     /locus_tag="PLK64_09020"
     CDS             complement(6062..6787)
                     /locus_tag="PLK64_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPV79929.1"
                     /translation="MVNDLRSGASAAPRATPGAAASPPAPLAAEAPIRGQRLPRSARR
                     AQLLEAAQGAFVESGYYAAAMDDIAERAGVSKPVLYQHFPSKLELYLALLDDRAGELV
                     AAVRSALASSTDNKARVFATVAAYFEFVAKEGSAFRLLFESDLINEDAVSSRIDGALT
                     QCGEAFADAIAADTNMSRADAMVLAMALTGMAQVAARNWATLDTTVGRAEAARLVGQL
                     AWRGLGGFPKAPAEGAGEHPATS"
     gene            6912..8090
                     /gene="moeB"
                     /locus_tag="PLK64_09025"
     CDS             6912..8090
                     /gene="moeB"
                     /locus_tag="PLK64_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HPV79930.1"
                     /translation="MDTGSGPPPDVLDDAEARTRYARHLLLPEVGLAGQRRLAAARVL
                     VLGAGGLGSPALLYLAAAGVGTIGIADDDVVELTNLQRQIVHGFPDVGRSKVSSAAAA
                     VARVNPGVRVETFDQRVSAQTADALVAAYDVVLDGTDNFPTRYAVNDACAHAGIPLVW
                     ASVLRFDAQVAVWWAGHGPCLRCVFPHQPAEGDVPSCAEAGVLGALCGTIGSLQAAEA
                     LKLVLGIGEPLVGRMLVHDALRQSWDTLALQADPECPACGSPVGGGRGARLGAVALAL
                     PVPAEPVAVVTARELSIEMRRPHPPVLIDVRPEPERTIVSIPGAVAIDLADFRSGEAF
                     SRPELGSLGTPLVLYCKTGVRSTEATRLVAAAGWGYAANLTGGVLAWVREVDPGLATY
                     "
     gene            8162..>8362
                     /locus_tag="PLK64_09030"
     CDS             8162..>8362
                     /locus_tag="PLK64_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HPV79931.1"
                     /translation="MSDGWGRVGEGRGGELPGIPPHAEAVLVLARSIPAGQVATYGDL
                     ARLVGEGGPRQVGQVMSRYGSDA"
BASE COUNT         1212 a         2733 c         3112 g         1305 t
ORIGIN      
        1 gtcacggcca ccacgtccgc cgcgcccgcc gctgtcgcga ccgccacgac cgccgtcacg
       61 accgccaccg tcgcggccac cgcggccacc gtcgcgtcca ccgccaccgg ggccgcggtg
      121 cccgccggac cgcccggtct cgccgaggtc ctcaagctct tcggcgtcga gaccggcccg
      181 ggtgcgcttg gcgttgggga gatagccgga cacgtcggag gggatgtcga gctcggcgta
      241 gaggtgctcg gaggaggagt aggtctcgac cggctccggg atgcccagat cgagctggcg
      301 atcgatcatc gaccagcgcg gcatgtcttc ccagtccacc agggtgatgg caatgccggt
      361 gttgccggcc cgaccggtcc ggccaatccg gtggacgtag gtcttctcgt cctcggggca
      421 ctggtagttg acgacgtggg tgatgtgctc gacatcgatg ccgcgggcgg ccacatcggt
      481 cgccaccagg acgtcgacgt tgccatgccg gaaggcgcgc agcgcctgct ctcgggcgcc
      541 ctgtccgagg tctccgtgga tggcggcggc ggcgaagccg cgctcgacca actcgtccgt
      601 cactcgggcc gccgctcgct tcgtgcgggt gaagatcaac acgagccccc ggtcgcgggc
      661 ctgcaacacg cgcgagagca actcgacctt gtcgagggag tgcgcgcggt agatgaactg
      721 ctcgatcgcc ttgaccgtgt gggcgttctc ggtctcgtcg cccatcgcgc ggatgtgcgt
      781 cggctgggtc atgtagcgcc gcgccaaggc gacgatcgcg ccgggcatgg tcgccgagaa
      841 aagcatcgtc tggcggccgg ccggggtgag cgacatgagg gtctcgacgt cgggcaggaa
      901 gcccaggtcg agcatctcgt cggcctcgtc gagcacgacg atccgggcct gcgacaggtc
      961 gaggtgcccc tgcttggcca ggtcgatgag gcggcccggc gtgccgacga cgatctcgac
     1021 gcctcgcttg agggcctcga cctgcggctc gtaggcgcgg ccgccgtaga ccgtgagcac
     1081 ccggacaccg cgctgcttgc ctgcgcgctc gaggtcactg gagacctgga cggccaactc
     1141 acgggtcgga gcgacggcca gcgcctgcgg cttgcccggc ttggccaggt cggcccaccc
     1201 ctcgtcagcc ggcgtgacga cccgctggat catcgggacg ccgaagccga gggtcttgcc
     1261 ggtgccggtc ttggcctggc cgatgatgtc gtgcccgccg agggcgaccg gcaacgtcat
     1321 ggcctggatg gggaaggggt ggatgatccc gaggtcgctc aaggaggcga cgatgtcggg
     1381 gtggacgccg aagtcggcga aggtgggctc ggtttcttga ggcaccaaag gagtcacgcg
     1441 aagatgtcgc ttccgatcgg gccgagatcg cggcgcgctc agggcgtgcg gccgattcgg
     1501 cgtgaggggc cgatcggagg tcttcgtggg aggtcgccga gccactcggc agtgtcgatt
     1561 cgggccgatc gggcaccgtg gtccactcaa gtctagccga ggagactccg gccgcccgta
     1621 tgccgcccta cgtccacccg gtatgccgcc ctaccggacc tccgtggcgc tgcccgcgtc
     1681 caccgggtcc gccccgtcag gcagcgccag gcgccatagg agtttggtgc cctccccaac
     1741 gttcgctgct ggcacctcgg cagcgaagcg gacgggcaca gtggcgggca ggtacggccg
     1801 cccagccgag tcgcgatagg gcacggccgc gcagttgagg gcgtagcggt cgttcgtccc
     1861 gtccgggggt tgtgtgtagt cgccgaccag gatgtcgggg cagggctcca gcgggatgtc
     1921 gcggggcgcc gtcagcgtga cagtgaggcg aatgggctca ccgacggcca ccaccgacgg
     1981 cgcgtccatg gtcgcggtca ctccggcgta tgccgttgtc acgagttcat ctctgacctc
     2041 agcgggcttg aatgtcgaca cgccgaacgg cgtcttcacg acggttccgg gttccatctg
     2101 acagccaggc gactggccga agccgggaac ggtcacccct cccgctgaac tcggccaggt
     2161 cagccgcacg tgggtctgtc ggacggcttg cccacaccag ttagaccaga acagggtcaa
     2221 agccgccggt acatccggcg ccaccagaac cgggtgcgga tagatccccc caggggcgtc
     2281 gctgacgacc gtcccatgaa ccgacgggat cgacaccgca gcgccattga ccagcgacac
     2341 gacggtcgga aagccctcca gccggcacgc ggccgtcccg gtgcggctga cgatctgcac
     2401 gaacaggtac atcgtcccgg tcgccccctc gatgtcgctc gtcgcggtga ggtcggcggc
     2461 gcggcatacg ggaaggcctt gggcgagtgc ggggtccggt gaggcgggga cggtcgtcac
     2521 tgggatgagg gggttggtcg caggcagggg cacccacggg acaaccccgt tgttgaccgc
     2581 cgatccggtg gcggtcgcgg acgacgtggc cgtcgtcgtg gcggaggcgg tcggaccacc
     2641 cacgctgcca aggtcggtgc cgccctggcc gagtcggacg gcgacgacca cgacggcgag
     2701 caccgcagcc gcggcgacga gcggcagcca gccggcgacg ccccgtccgc ggcggccgaa
     2761 ccccgccgag tgagtgggca ggtcggtggg caccgcgggc ggtgggggga gggcgtcgcg
     2821 ccagcgcgcg gcatacccct gcagttgctc gtcgatcggg tcgggacggt cagtcatcga
     2881 agtctcccaa cagctcgcgc agtcgcgctc gggcctgggc aaggtggctt ggacggtccc
     2941 cggcgcgcag cccatcgttt gggcgacgga ggcgatgtcg aggccgacga agtagtagag
     3001 gtcgaccgcc tgacgctggc ggtgcgggag tcgggcgacg gcgcgctcga ggtcgacgtc
     3061 gggctcggtc ggccccgcgg tcagcgcgtc atcggccagc agggtggtcg gccggggccc
     3121 gcgggtgcgg tgccggcggg cctgatcggc gacgatcgcg agcaaccagg gggcggcgga
     3181 gcctcgggcc ggatcgtagg ttgcccgccg cgaccacgcc cgcacgaggg cctcctggac
     3241 gacatcgtca gcggcagccg cgggcacgag gcggtgggca tagcgggaca gcgccggcag
     3301 gtggggagcg acccaggccc cgaacgcggc tgcgtcgggc atgtcatggg tatgccgccc
     3361 cactccggtc atcgtcacac ccctcttacg ccgggagcga ggtggtccgt gtagtgcccg
     3421 cgcccaacac cactgtgtca tctctggtgc aggcggggcg gaaggtgcag ggctgacgag
     3481 ccgtgcacaa ctcagtgggg cccagccaaa cggctaggcc ccacttggtg ttgttggagg
     3541 tgtgtcgatg ttggtcgccg catttgggca gcgggcatta ctcaggcgca gctgcaggaa
     3601 tgcccgctgc aattctcgac ctgccatccc cgaaatggca tggctgagta tgcgtcggtg
     3661 aatggactgt agtgacgttc ggatcgaggc gccctcagat agtcgtgagg ggacgatcag
     3721 gtcgatcgtt agtggaagac ctctgtcagt ggaaggccac tgtcagtgga agaccaccgt
     3781 caagggcgtg tagccactgt tggcgtagag gtagccgttg tcggtcgacc agtacgacag
     3841 ttgggcgttg gtgtagagcg aggtgagcac gttgccgttc aggacccagc ctgtcgctcc
     3901 gatccagttg tagaacccga cgtagtggta gccgtcgctc accgacaggt accgagaagt
     3961 gtctgcggcg ttgcggaagt agagaatacc gccatagagg tacgactcga gcgagagggc
     4021 cgcgtcgctg ccggtgacga cgcaccactg gccgccgttc tcaccgagcc acccgatgga
     4081 cgcaccgcgg gcgaagagtt cgaccttctt ggctgtgccg gaagaggccg aagccgccag
     4141 gtgcgactct gcgacatggt caccgaacgt gccggtgggg gcggtgggtg ctgcagtgga
     4201 catggctttc tccattcgaa tgatgagttg gagttgcccg gccgggttgc cggaggtcct
     4261 catgaattgc tggggagagt gaggacgtct ctgacgtgaa tgtagcaccg ctgtgccgcc
     4321 agcgagccct acggcccaaa ggaattcggt ccttctttgc tttccgtgcg ccgagtgcgc
     4381 cgctgaaggg cgaccgcacc tgtcaatgac tcgggcggcc cgttggtcgg ttgaggcacc
     4441 gccgatacag tcagccctat gaccgatgac accggggtcg cagggagtgc tgccgtgggc
     4501 gaggtgccgc acgaggccgt cgtcgacctg ctgggagcgc tgagctatgc ccagctcagc
     4561 gggttcgagg cactcgcggc tgacgccgcg atgaccccgc acctgcgcgc caagctggcc
     4621 ttcgcccgga tgggggtgga gatgttccac cggtgcgagc aggtggggca gcggctgcgc
     4681 gaactcgggg ccgaccatga gagcgccatg gcccccttcg tcacctcggt cgacgccttc
     4741 cacgagcgca ccaagcccag cgactggctg gagggcgtcg tcaaggccta cgtcggcgac
     4801 gggatcgtcc gcgacttcta ctccgagatg gccacccacc tcgacccggc gaacgaggcg
     4861 ttcgtcgcgc agatgctcgg acaggccggt ggcgccgaag cgatcgtcca cgaggtgcgc
     4921 gaagccatcg ccggtgacgc ccggcgcagc ggccggctgg cactgtgggc tcggcggatc
     4981 atgggtgaag cgatccatgc gacccagagc gtggccgtcg aacgcgactc gctgaccgcg
     5041 ctcctggtcg gcggcgagcc ggggtcggac ctcaacgagg tgggccggat gttcgcgcgg
     5101 atctcggcgg cccacaccgt gcggatggag gcgttgggcc tgtcggcctg atcgtcccga
     5161 cggcgctggt cagcacaggc agtaagaacg cgccggggga cagcagtggg caacagcagt
     5221 ggggcccagc cgttttcggc tgggccccac tgctgttgtc ggcgcttggc ctcaggcctt
     5281 gctggtgacc ttgccgtaga tgacgatgcc gacggcggcg atgatcgtgc tgatgatcca
     5341 gcgcagccag tcgatccccg acgtggtctg aacgccgagc ttgccggcga cccaccagcc
     5401 gatgagagcg gcgacgatgc cgatcgctgc cgtgatcggc aggctcagcc cctgccgtcc
     5461 aggcaggatg aaacgtgcca gcagtccgac gatcaaaccg ccgacgatgg cccccaggat
     5521 gctcatgtcc actccttctg gccgctcccg cggccgctgt caagcccggt ggtccgggtg
     5581 ggggcgggct ccccaggatt caaaactctc acgaagtgag acgcattcct gggattgccg
     5641 ctcgtgatct tgacgtgacc tggtggcagg gccgttcggg acgagggttg ctgaggcccg
     5701 acgtcagtgc gcgccgaagc cgaccttgcc cttctcggcc tctccgacgt ggacgaagcc
     5761 gatgacggca gcggggatga agtagcgccg tcccttgtcg tcggcgaggt cgagcacgct
     5821 gccatcgcgc agcgcgacgg ccacggcggc gccgatggcg gccggggtct cgttggtctc
     5881 gaacgcaatc tcgcgggcga catcgcgcac gccgatcctg acctccacgt ggggttccct
     5941 tcgtcagttc acgtatgccg tgtgctcgcc tcgcgactcg ccgccccctc gcggaggtga
     6001 cggtggcacg gggaacccgg gcgcacggca tacccgggtt ggggtgtcga acccctcaac
     6061 tctaggaggt ggcggggtgt tccccggcgc cctcggccgg tgccttgggg aagcccccca
     6121 acccgcgcca ggcgagttgg ccgaccaggc gggccgcttc ggcgcggccc acagtggtgt
     6181 cgagggtggc ccagttgcgg gcggccacct gtgccatccc cgtcagggcc atcgccagga
     6241 ccatcgcatc ggcgcgggac atgttggtgt cggcggcgat ggcgtcggcg aaggcctcgc
     6301 cgcactgggt cagcgccccg tcgatgcggc tgctgacagc gtcctcgttg atgaggtcgc
     6361 tctcgaagag gagccggaag gccgacccct ccttggcgac gaactcgaag taggcggcga
     6421 ccgtcgcgaa gacccgtgcc ttgttgtccg tggaactggc cagcgcgctg cgaacggcgg
     6481 ccaccaactc gccggcgcga tcgtccagga gtgcgagata gagctccagc ttgctcggga
     6541 agtgctggta aagaacgggt ttggacaccc ctgcgcgttc ggcgatgtcg tccatcgcgg
     6601 cggcgtagta gcccgactcg acgaaggcgc cctgggcggc ctccagcagt tgggcccggc
     6661 gggccgagcg cggcagccgt tggccgcgga tgggtgcctc ggcagcgagc ggggcgggcg
     6721 gcgaggccgc cgctcccggc gtcgcacgcg gggcagccga cgctcccgat cgcaggtcgt
     6781 tcaccaccgt caacaccacc ttgtgcgtat cgcacgactg tgcgaacgat cgaccgccga
     6841 cgaccgtccg gccgccaggt gccatcgtac tcaccggtat cccccagccc tatgacgtaa
     6901 cctcgctagc gatggacacc ggatccggcc cgccgcccga cgtgctcgac gacgccgagg
     6961 cccgcacgcg ttatgcccgg cacctgttgc tgccggaagt gggcctcgcc ggacaacggc
     7021 ggctcgctgc ggcccgggtc ctcgtcctcg gggcgggagg gctgggctcg ccggcgctgc
     7081 tctacctcgc tgccgccggg gtcgggacca tcgggattgc cgatgacgac gtggtcgagt
     7141 tgaccaacct gcagcgccag atcgtccacg gcttccctga tgtcggccgg tccaaggtgt
     7201 cgtcggctgc ggcggctgtg gcgcgggtca accccggcgt gcgggtcgag accttcgacc
     7261 agcgggtgag tgcgcagacc gcggacgccc ttgtcgcggc atacgacgtc gtgctcgacg
     7321 ggaccgacaa cttcccgacg cggtatgccg tcaacgacgc ctgcgcgcac gcgggcatcc
     7381 ccctcgtgtg ggcgtcggtg ctgcgtttcg acgcgcaggt ggcggtgtgg tgggccgggc
     7441 acgggccatg cctgcggtgc gtgttcccgc accaaccggc cgagggcgac gtgccgtcct
     7501 gcgcggaggc gggggtgctg ggggccttgt gcgggacgat cggctcgctg caggccgcgg
     7561 aggcgctcaa actcgtgctg ggcatcggcg aacccctcgt tgggcggatg ctcgtgcacg
     7621 atgcgttgcg acagagctgg gacacgcttg cgctgcaggc agatccggag tgcccggcct
     7681 gcgggtcgcc cgtcggggga gggcgtgggg cgcggctcgg ggcggttgcg ctcgcgctcc
     7741 ctgtcccggc tgagcccgtt gcggtggtga ccgcccgcga gttgtcgatc gagatgcggc
     7801 ggccgcatcc gcccgtgctc atcgacgtgc gcccggagcc cgagcgtacg atcgtctcga
     7861 tccccggagc tgtggcgatc gatctggcgg atttccgctc gggagaagct ttttcacgac
     7921 ccgagctggg gagtctgggg actccgttgg tgctctattg caagaccggg gtgcgttcga
     7981 cggaggccac tcggctggtg gccgccgccg gctgggggta tgccgcgaac ctcaccggcg
     8041 gggtgctggc ctgggtgcgc gaggtcgacc cggggctggc gacctactga tggctgtggg
     8101 agggacgccc ggggcagggc ggtcggccgg ggagcccgcc gggtcgcggt cggacgttcc
     8161 ggtgtcggac ggttggggtc gcgtcggcga gggtcgcggc ggcgagctgc cggggattcc
     8221 gccgcatgcc gaggcggtcc tggtgctcgc gcgatccatc ccggcggggc aggtggcgac
     8281 ctatggcgat ctggcgcgat tggtcggcga gggcgggcca cgacaggtgg ggcaggtgat
     8341 gagccggtac ggctcggacg cc
//
LOCUS       DAOMZY010000138         8253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_8170_length_8253_cov_5.579288, whole genome shotgun sequence.
ACCESSION   DAOMZY010000138 DAOMZY010000000
VERSION     DAOMZY010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 8253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8253
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8170_length_8253_cov_5.579288"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="PLK64_09035"
     CDS             complement(<1..1008)
                     /locus_tag="PLK64_09035"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HPV79932.1"
                     /translation="MLIGVPRESRPGETRVAATPKTVEQLLKLGYQVAVEAGAGAAAS
                     FDDAAYAAAGATVAHPATVWGADIVTKINPPSDEEIALLRQGAILVSLAQPALNPALV
                     DKLAAAGVTAMAMDAVPRISRAQALDVLSSMANIAGYRAVIEAAHEFGSFFTGQVTAA
                     GKVPPAKVFVSGAGVAGLAAIGTANSLGAIVRATDVRPEVGEQVESMGAEFVKPDVPE
                     QEVSSDGYAKEVGADYAAAAAVMYAAQAKDCDIIVTTALIPGRPAPRLITEEMVAAMK
                     PGSVIVDMAASNGGNVAGSVADKMVVTENGVKIIGYTDLPGRLPTQASQLYGTNVVNL
                     MK"
     gene            complement(1225..2181)
                     /locus_tag="PLK64_09040"
     CDS             complement(1225..2181)
                     /locus_tag="PLK64_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HPV79933.1"
                     /translation="MPAITVPDLSVLPSIPAPGPAATQRPVVSRTTAPHGLEGEGFPV
                     KRAFAGVDLSLLDPFIMMDEMGEVDYGPGEPKGTPWHPHRGFETVTYIIDGTMDHADS
                     YGGGGTITNGATQWMTAGSGILHIEAPPEQLVASGGLFHGLQLWVNLPRTDKLATPRY
                     QDLAATDVALLCSPDGGALVRVIAGDLAGHRGPGDTHTPITMVHATLAPGARLDLPWR
                     RDFNALVYVMGGAGTVGPADAPLRAGQLAVLGTGDALTVSADPTQESRYAAGLDVVVL
                     GGAPIREPIAWAGPFVMNTRAEVVSAFEDYQAGRLGHPLPHA"
     gene            2330..2704
                     /locus_tag="PLK64_09045"
     CDS             2330..2704
                     /locus_tag="PLK64_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019619145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HPV79934.1"
                     /translation="MEPVTHLAALGDSLLATARDAESGRAASTLFAAPQLRAVVIALK
                     EGAALAEHNAPPAATLQCLSGQARLWATGREWVLDAGGYAAIPPERHGVDALTDCVLL
                     LTVSGTRAPEPVEVTLEASAPA"
     gene            complement(2870..4615)
                     /gene="argS"
                     /locus_tag="PLK64_09050"
     CDS             complement(2870..4615)
                     /gene="argS"
                     /locus_tag="PLK64_09050"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPV79935.1"
                     /translation="MPSPHDTLAPLVSAAIATAFGDELRGADPVLRPSQFADLQANFA
                     LPLAKRLGMPPREVATRVLAALDLTGVCSGIEVSGPGFVNLTLDDSWIASSVQAVGAD
                     PMLGVTGDAPRVVVIDYSAPNVAKEMHVGHLRTTVVGDALARTLEHLGHRVIRQNHVG
                     DWGTPFGMLVEHLVEVGEGSSEALVVETDPNAFYLAARTKFDAEGSFADRARARVVAL
                     QAGDPETLRLWGELVGLSKSYFNRIYAALEVTLTDTDLAGESTYNDALAGICEELEAK
                     GIATISDGALCVFLPEFTGREGKPVPLIVRKSDGGYGYGTTDLATIKHRVEDLGADRV
                     LYVVGAPQSLHFQMVFATARLAGWLPDTVEVVHVQIGNVLGDDRKILRTREGKALRLG
                     ALIEESVDAAQAVLDEARPDLDPATRHTLAREIGVGAIKYADLSVAHDSEYVFDLARM
                     VSLTGNTGPYLQYAAARVRSIFRSAGVEIEVPVGGAQRRIVVTEPAERALALALLEFG
                     SVVRGVGAELEPHRLCGYLFDLAQTFSAFYEACPVLKAPDAETRESRLGLCATTFRVM
                     EQGLALLGMATPAQM"
     gene            complement(4656..5198)
                     /locus_tag="PLK64_09055"
     CDS             complement(4656..5198)
                     /locus_tag="PLK64_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HPV79936.1"
                     /translation="MPPAPAPLGTLDWRPLADHLDLVAGPVAAAAGLVPDALVAPIDA
                     TLADTAAFCAAYQVPAEASANCVVVEGRRAERVVRAAVMVLAVDRADVNKVVRKHLDV
                     RKLSFMGSEEAQEATGMIQGGITPVGLPSDWLILVDSAVVAAGPVVIGGGVRGAKLLL
                     HGGDLAALPGAAVLDLAVPR"
     gene            5283..6137
                     /locus_tag="PLK64_09060"
     CDS             5283..6137
                     /locus_tag="PLK64_09060"
                     /inference="COORDINATES: protein motif:HMM:NF025580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HPV79937.1"
                     /translation="MSTVPYDPAAAFPIGAPEGWRADPNLADTERFWDGLHWTDETRP
                     SVRPGTYARTQQALAEGGRSGSRALGGVVQGLLTLCGLLAAALLLYTIIVFNALSVWR
                     LQPTPAAEQEVDKLLLLSTLATWADLGLRTITGILFLVWLGIRYTDTRVDHRVLRRST
                     GVAIFSWIIPVLQLWWPAQAVKDLWHASRPDAARLGGVGARLPVPSVFFVWWPAWLLA
                     GSGSTAVMTVLAQPQFELTWLIWGTIGTGVQQLATLIAAWALIVIINQVEDHLVADDP
                     TAAFDGNI"
     gene            complement(6186..8048)
                     /locus_tag="PLK64_09065"
     CDS             complement(6186..8048)
                     /locus_tag="PLK64_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPV79938.1"
                     /translation="MADGIPEVGRRASAEPDAAAWEPVVAALPWSRPPDRLWDDGPRH
                     GTWFPGGRINAATACISDHARRTPEHVALLWEGEPGDRRELTYAALDADVTALARALR
                     GLGLGVGDVVALHLGWMPETVVAMFACARIGAVFAVVPTPLPPEALADRLRAIGARLL
                     FTQDGAWRRGTVLPLKARADEALSALGGIEHTIVVRRTGIDVGWYEGDRWYHDLLVGV
                     RPGLRREPSEADTPADLPADHPLQVVSLANRRGQPLTVALGTGHTLAIALALHHAGIA
                     DGSILWCAGENSWLGTQVHGVYGPLVAGHTAVLYEGTVDVPTHARAWELMRRHGVTTL
                     VTTPSVLRAMRSWSFELAIGDEPIGAVPQLRRVVAFGEHMDAEVREWAATGLAGQPVS
                     VADGWGQVELGGIVLLDNPIDASAMPDLGRVLVDRSGEVAPDGMPGELVLTRPWAGTL
                     VGVSGPRETLDDAHWSRLPGSYTTGDVARSEPDGGLEFLGRTDEVTSVSGQLVSLSEV
                     RDVLLEHPFVSAADVVERRDQLRGRVIAAAVVLTPDGVASDPTLVARDLGRSVRESLG
                     GLARPRVLMLVDRFGDELRGVERRRALAGIPVPEGPEPRRVTWEQLLVAADHLT"
     gene            complement(8041..>8253)
                     /locus_tag="PLK64_09070"
     CDS             complement(8041..>8253)
                     /locus_tag="PLK64_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79939.1"
                     /translation="DWAKAAWLRADGPFPGAPPEVGVPVINAAGMPLATLVADLPLTA
                     SPEQIEAAVAHLTRAVAAAVRTLGHG"
BASE COUNT         1201 a         2963 c         2917 g         1172 t
ORIGIN      
        1 cttcataagg ttgacgacgt tggtgccgta gagctggctg gcctgcgtcg gcagtcgacc
       61 cggcaggtcg gtgtaaccga tgatcttcac gccgttctcg gtgacgacca tcttgtcggc
      121 caccgagccg gcgacgttgc ctccgttgga cgccgccatg tcgacgatga ccgacccagg
      181 cttcatcgcg gcgaccatct cttcggtgat cagccgaggg gccggtcgac cggggatgag
      241 cgcggtggtg acgatgatgt cgcagtcctt ggcctgcgcg gcatacatca cggcggcggc
      301 cgcggcgtag tccgcgccga cctccttggc gtagccgtcg ctgctgacct cttgctcggg
      361 cacgtcgggc ttgacgaact cggcgcccat cgactcgacc tgctcgccga cctcgggccg
      421 cacgtcggtg gcccgcacga tcgcgccgag gctgttggcc gtgccgatcg ccgcgagccc
      481 ggccacgccg gcgcctgaga cgaagacctt ggccgggggc accttgcccg ccgccgtcac
      541 ctgaccggtg aagaacgacc cgaactcgtg cgccgcctcg atcaccgcgc ggtagccggc
      601 gatgttggcc atcgacgaga gcacgtcgag ggcctgggcg cgcgagatac gcggcacggc
      661 gtccatggcc atcgccgtga cccccgccgc cgcgagtttg tcgaccagtg ccgggttgag
      721 ggccggctgg gccaggctca cgaggatcgc gccctggcga agcagcgcga tctcctcgtc
      781 ggagggtggg ttgatcttgg tgacgatgtc ggcgccccag acggtggccg gatgggcgac
      841 cgtggcgccg gctgcggcat acgcggcatc atcgaaactt gccgcggcac ccgcgcccgc
      901 ttcgacagcg acctggtacc cgagtttgag aagttgttcg acggtcttgg gggtggcagc
      961 gacccgggtc tcacccggcc gcgactcccg agggacaccg atgagcacgt gttctccctg
     1021 cgtcggcgtg tgtccccgca gcgcgagcgc actacggcat gccgggtcga cccggcgatg
     1081 tcacaaacct atggggttgg cccggaggga acaaagggac ctaggtcagt gggagaggtc
     1141 tcacaaccca tggggtatgc cgcccgctca ccttcgcgtc ccgaccaccc tgccgtccac
     1201 cccgccgggc ggccagggcg gcgatcaggc gtgcggaagc gggtggccga gccgcccggc
     1261 ctggtagtcc tcgaaggcgg agacgacctc ggcgcgagtg ttcatgacga acggcccggc
     1321 ccaggcgatc ggttcacgga tcggggcacc gccgaggacg acgacgtcca ggcccgcggc
     1381 ataccggctc tcctgggtgg ggtcggcgct gacggtgagg gcgtcccccg tgccaaggac
     1441 ggccagttgc ccggcccgca gtggcgcgtc ggccgggccc acggtgccgg cgccgcccat
     1501 gacatagacg agggcgttga agtcccgacg ccacggcagg tcgaggcgtg cgcccggcgc
     1561 gagcgtcgcg tggaccatgg tgatcggcgt gtgggtgtcc cccggcccgc ggtggccagc
     1621 gaggtcaccc gcgatgaccc gcacgagcgc acctccgtcg ggcgagcaca gcagggcgac
     1681 atcggtcgcc gcaaggtctt ggtagcgcgg ggtggccagt ttgtcggtgc gcgggaggtt
     1741 gacccacaac tgaaggccgt ggaaaagccc tccgctggcg acgagttgct cgggaggggc
     1801 ttcgatgtgc aggatccccg acccggccgt catccactgc gttgcgccgt tggtgatcgt
     1861 cccgccgccg ccgtaggagt cggcgtgatc catcgtgccg tcgatgatgt aggtcaccgt
     1921 ctcgaagccc cgatgcgggt gccacggtgt gcccttgggc tcccccggcc cgtagtcgac
     1981 ctcgcccatc tcgtccatca tgatgaacgg gtcgagcagg ctcaggtcga cccccgcgaa
     2041 cgcccgcttg accgggaagc cctcaccctc gagcccgtgc ggcgccgtcg tgcgactgac
     2101 gaccgggcgc tgcgtggcgg ccgggccggg ggccgggatc gacggcagga cgctgaggtc
     2161 cgggacggtg atggctggca tgcggccctc ctttgagggt gtgcgtggac cgaagatagt
     2221 tttgatttca actatatcct aggtgcccga tttgcggcaa ctctcccgcg tgtccaactg
     2281 gctcgcggct tgacgaccgc gcgctgcttg ggcggcatac cgtgtgggga tggaacccgt
     2341 gacacacctc gccgcactcg gtgactccct cctggccacg gcccgcgacg ccgagagcgg
     2401 gcgggccgcc tcgacgctgt tcgccgcgcc ccaactgcgc gcggtggtca tcgcgctcaa
     2461 ggagggcgcg gcgctcgccg agcacaacgc cccgcccgcc gccaccctgc agtgcctgtc
     2521 cgggcaggct cggctctggg ccactggtcg cgagtgggtc ctggatgccg gagggtatgc
     2581 cgcgatccca cccgagcggc acggcgtgga cgccctcacc gattgcgttc tcctgctcac
     2641 ggtgtctggc actcgggcac ccgaaccggt cgaggtgacg ctcgaagctt cggcacctgc
     2701 ataaccatgg ggcccggcgt tgacggccgc gagtgagtgc ctgacggagt gcgcttggtg
     2761 gggtgatcac cccgatgatc gcactccgtg gcgcacgcgg ctgaagcggc gcggcctcgc
     2821 acgcagccgc gtcaagccct cgcacacggc cgcgtctcgc cccccgacgt cacatctggg
     2881 cgggcgtcgc cattccgagg agcgccaaac cctgctccat cacccggaag gtcgtcgcgc
     2941 acagcccgag ccgcgactcg cgcgtctcgg cgtcgggggc cttgagcacc gggcacgcct
     3001 cgtagaacgc cgagaacgtc tgggccaggt cgaacaggta gccacacaac cggtgcggct
     3061 ccaactcggc cccgacgccc cgcaccactg agccgaactc cagcagcgcc agcgccaacg
     3121 cccgctcggc cggctcggtg acgacgatcc gccgctgcgc tcccccgacc ggcacctcga
     3181 tctcgacccc cgccgagcgg aagatcgagc gaacccgggc ggcggcatac tgcagatagg
     3241 ggccggtgtt gccggtgagc gagaccatcc gggcgagatc gaagacgtac tccgagtcgt
     3301 gggcgaccga gaggtcggcg tacttgatcg ccccgacgcc gatctcccgc gcgagggtat
     3361 gccgtgtcgc cgggtcgagg tcgggccgcg cctcgtcgag gaccgcctgg gcggcgtcga
     3421 ccgactcctc gatgagtgcc cccaggcgca gggccttgcc ctcccgggtg cgcaggatct
     3481 tgcggtcgtc gccgaggacg ttgccgatct ggacgtggac gacctcgacg gtgtcgggca
     3541 accacccggc caaccgggcg gtcgcaaaga ccatctggaa gtgcagcgat tgcggggcgc
     3601 cgacgacata gaggacccgg tcggccccga ggtcctcgac gcgatgcttg atcgtcgcga
     3661 gatccgttgt gccgtaacca tatccaccgt ccgacttgcg gacgatgagc ggcaccggct
     3721 tgccttcgcg gccggtgaac tcggggagga acacgcacaa cgcgccgtcg gagatcgtcg
     3781 cgatcccctt ggcctcgagc tcctcgcaga tcccggcgag cgcgtcgttg taggtcgact
     3841 cgccggccag atcagtgtcg gtgagcgtca cctcgagcgc cgcgtagatc cggttgaaat
     3901 agctcttgga caagccgacg agctcgcccc agagccgcag ggtctcgggg tcgccggcct
     3961 gcagcgccac gacgcgcgcc cgggcccggt cggcaaacga cccctcggca tcgaacttcg
     4021 tccgggccgc gagatagaag gcgttggggt cggtctcgac gaccagcgcc tcggacgagc
     4081 cctcaccgac ctcgaccagg tgctcgacga gcatgccgaa cggcgtgccc cagtcaccga
     4141 cgtggttctg ccggatcact cggtgcccga gatgctcgag cgtgcgggcg agcgcgtccc
     4201 ccaccacggt cgtgcgcagg tgcccgacgt gcatctcctt ggcgacgttg ggcgccgagt
     4261 agtcgatgac gacgacccgc ggcgcatcgc ccgtgacgcc gagcatcggg tcggccccga
     4321 cggcctgcac cgacgacgcg atccatgagt cgtcgagggt gaggttgacg aaccccgggc
     4381 cgctcacctc gataccgctg cacactccgg tcaggtcgag cgcggccagc acccgcgtcg
     4441 cgacctcgcg cggcggcata ccgaggcgct tggccagtgg caacgcgaaa ttggcctgca
     4501 ggtcggcgaa ctggctgggg cgcagcaccg ggtcggcacc gcgcaactcg tcgccgaacg
     4561 cggtcgcgat cgcggccgag accaaagggg cgagcgtgtc gtgcggggag ggcatccccc
     4621 aagggtagtg gcgcgggaca accgctttcc ccggctcagc gcgggacggc gaggtccagc
     4681 acggccgccc ccggcagcgc cgcgagatcg ccgccgtgca gcaacagttt cgcgccgcgc
     4741 acgccgccgc cgatgaccac cggccctgcc gcgaccaccg ccgagtcgac gaggatcagc
     4801 cagtcggagg gcagccccac cggcgtgatc ccgccctgga tcatcccggt ggcctcctgc
     4861 gcctcctcgg accccatgaa cgagagcttg cgcacgtcga ggtgcttgcg gaccaccttg
     4921 ttgacgtcgg cccggtcgac ggcgagcacc atgacggccg cgcgcaccac ccgctcggcc
     4981 cgacgtccct cgaccaccac acagttcgcg gaggcctccg ccggcacctg gtatgccgcg
     5041 cagaacgccg cggtgtcggc cagcgtcgcg tcgatgggcg ccaccaaagc atcgggcacg
     5101 agccccgcgg cagccgcaac cggaccggcg accaggtcga ggtggtcggc gagcggacgc
     5161 cagtcgagag tcccgagcgg agcgggtgcg ggcggcatac cggggatgct agtgggtcgt
     5221 ccgaacgggt ggatttcggg ccaggactcg acctccccgc ccaccgcgtc aaagatgggc
     5281 gcatgtcgac ggttccttac gacccggccg ccgcgttccc gatcggtgcc ccggagggct
     5341 ggcgcgccga ccccaatctc gccgacaccg agcggttctg ggacgggctg cactggaccg
     5401 acgaaactcg gccgtcggtg cgccccggca cctatgcgcg gacccagcag gcgttggccg
     5461 agggcggacg aagcggctcg cgggcgctcg gcggggtggt tcaggggctg ttgacgcttt
     5521 gcggcctctt ggccgcagcc ctgctcctct acacgatcat cgtcttcaac gccctctcgg
     5581 tgtggcggct gcagcccacg ccggccgccg agcaggaggt cgacaaactc ctcctcctgt
     5641 cgaccttggc cacctgggca gatctggggc tgcgcaccat caccgggatt ctgttcctcg
     5701 tgtggttggg cattcgatac accgacaccc gggtcgatca ccgggtgctg cggcgttcga
     5761 caggcgtggc gatcttctcc tggatcatcc ccgtcctcca gttgtggtgg ccggcccagg
     5821 ccgtcaagga cctctggcac gcgtcccgcc cagacgccgc ccggctgggg ggtgtgggcg
     5881 cgcggttgcc cgtcccctcg gtgttcttcg tgtggtggcc ggcctggctc ctggccggct
     5941 cgggatcgac ggccgtcatg acggtcctgg cgcaaccgca gttcgagctg acgtggctca
     6001 tctggggcac catcggcacc ggcgtccagc aactcgccac gctcatcgcg gcctgggccc
     6061 tcatcgtcat catcaaccag gtcgaggatc acctcgtcgc cgacgacccg acggcggcct
     6121 tcgacggcaa catctgacgg acgcgggcgc ctgcgctctg cgctctgcgc cctgcgccct
     6181 gcggctcagg tgaggtggtc ggccgcgacc aacaactgct cccaggtgac ccgccgcggt
     6241 tcggggcctt ccggcacggg tatgccggcc agagcccgtc gccgctcgac ccctcgcaac
     6301 tcgtcgccga accggtcgac aagcatgagc acccgcggcc gggccaaccc gcccaacgat
     6361 tcccggacgc tgcggccgag atcccgggcg acgagggtcg ggtcggaggc cacaccgtcc
     6421 ggggtgagga cgacggccgc agcgatgacc cgcccgcgga gttgatcgcg gcgctccacg
     6481 acgtcggcgg cggaaacgaa cgggtgctca agcaacacat cccgcacctc gctgagcgag
     6541 acgagttgcc ccgacaccga ggtgacctcg tcggtgcggc cgaggaactc caggccgcca
     6601 tcgggctcgg accgcgccac gtcgcctgtc gtatacgacc cgggcagccg agaccagtgg
     6661 gcgtcgtcga gggtctcccg cggcccggac accccgacga gtgtgccggc ccagggacgg
     6721 gtgaggacga gctcgccggg catcccgtcg ggagccacct cgccactgcg gtcgacgagc
     6781 acccggccga ggtcgggcat cgcagacgcg tcgatcgggt tgtccagcaa cacgattccg
     6841 ccgagctcga cctggcccca cccgtcggcc accgacaccg gctgtccggc caggccggtt
     6901 gccgcccact ccctgacctc ggcatccatg tgctcgccga aggcgacgac ccggcgcagc
     6961 tgaggcactg cgccgatcgg ctcgtcgccg atggccagct cgaacgacca cgaccgcatc
     7021 gcccgcagca ccgacggcgt cgtgaccaag gtggtcacgc catgtcggcg catcagttcc
     7081 caggcgcggg cgtgggtcgg gacgtcgacc gttccctcgt agaggaccgc ggtatggccg
     7141 gcgacgagcg ggccgtagac cccgtgaacc tgggtgccga gccaggagtt ctcccctgca
     7201 caccagagga tggagccgtc ggcgatgcct gcgtggtgca gggcaagggc gatcgccagc
     7261 gtgtgccccg tgccgagcgc gaccgtcaac ggctgcccgc ggcggttggc caggctgacc
     7321 acctgcagcg ggtggtcggc cggcaggtcg gccggggtgt cggcctcgga cggttcgcgg
     7381 cgcagaccgg gacggacccc gacgagcagg tcgtggtacc accggtcgcc ttcgtaccac
     7441 ccgacgtcga tccccgtgcg tcgcacgacg atggtgtgct cgatcccgcc gagcgccgac
     7501 agcgcttcgt cggcccgcgc cttcaggggc agcacggttc cgcgccgcca ggcgccgtcc
     7561 tgggtgaaga gcagtcgggc gccgatggcc cgcagtcggt cggccaacgc ttcagggggt
     7621 agcggcgtcg gcacgacggc gaagacggcc ccgatccggg cgcaggcgaa catcgccacg
     7681 accgtctcgg gcatccagcc gaggtgcaag gcgacgacgt cacccacacc gagcccgagc
     7741 ccgcgcaacg cccgggccag ggcggtcacg tcggcgtcga gggcggcata cgtgagctcg
     7801 cgacggtctc caggctcacc ttcccagagc agcgcgacgt gctccggggt gcgtcgcgcg
     7861 tggtcactga tgcaggcggt cgccgcgttg atccggcccc cggggaacca cgtgccgtgc
     7921 cgcgggccgt cgtcccagag tcggtccggt ggccgcgacc acggcagggc cgcgacgacg
     7981 ggctcccacg cggcagcgtc cggctccgcg ctggctcgcc ggccgacctc cggtatgccg
     8041 tcagccatgg ccgagcgtcc gcacggcggc cgcgacggca cgggtcaggt gggcgacggc
     8101 cgcctcgatc tgctccggcg aggcagtgag gggcaggtcg gcgacgaggg tcgcgagcgg
     8161 cataccggcg gcattgatca cggggacccc gacctcgggg ggcgcgccgg ggaacggccc
     8221 gtcggcgcgc agccaggccg ccttcgccca gtc
//
LOCUS       DAOMZY010000139         8230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_8204_length_8230_cov_4.927951, whole genome shotgun sequence.
ACCESSION   DAOMZY010000139 DAOMZY010000000
VERSION     DAOMZY010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 8230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8230
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8204_length_8230_cov_4.927951"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="PLK64_09075"
     CDS             <1..1043
                     /locus_tag="PLK64_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPV79940.1"
                     /translation="TRLMTMKQPIGPTLMITPWNFPLAMGTRKIGPAIAAGCTMVVKP
                     AAQTPLTMLLLTQVLLECGVPAGVLNVVSTAHTGEVMEPIIRDPRLRKLTFTGSTQVG
                     QRLVEQSAQGLLRLSMELGGNAPFIVFDDADLDRAVDGAMLAKMRNIGEACTAANRFL
                     IHESVAEEFGRRFAERMAALRVGKGTVKGVDVGPLVDRKARESVDALVQEAVAGGAKI
                     LTGGGPLPGKGWFYAPTVLTDVPASSRIAVEEIFGPVAPISTFATEAEAIARANDTAY
                     GLVAYAFTRDLARALRVSEELEFGMVGINQGVVSNPAAPFGGMKASGFGREGGFEGID
                     EYLEVKYVGIAP"
     gene            1146..2174
                     /locus_tag="PLK64_09080"
     CDS             1146..2174
                     /locus_tag="PLK64_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HPV79941.1"
                     /translation="MSQQGGRSGARHRGITSLAELLASADGRMQDGARIGASIWPTGL
                     EPLDGHLGGGLRGGELTLIGGPQGLGKTTMALQMVRNTVAAGRSAIVFSFEHEGRILL
                     ERLIALEAATVAGRGSVTIRQLRDVFEEGSPHGGGLIERLAQLPGAAEGIAAQEAYAA
                     RLHLHESSGSTTDMDEIREIVAQLKEAGEEPLVLVDYLQKVPILGGRHVEEERVTEVV
                     EGLKELALDADLPVVAIVAADKAALVAGHRMRLHDLRGSSALAYEADIALILNGKYDV
                     VARHHLMFDVANAERFKNWVVVTVEKNRSGLADISMEFEKRFHEGRFEPKGRMVQEQL
                     IEGRVFLE"
     gene            2214..2705
                     /locus_tag="PLK64_09085"
     CDS             2214..2705
                     /locus_tag="PLK64_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HPV79942.1"
                     /translation="MGVVTSPMSRSTRQLLQLVAVGVVALVLVAAYLLARGGSAGTAG
                     VGAQPTARPTTSTQATGGTVDPASGLVWVDESTLPAQAKATLALIRAGGPYPYPRNDD
                     QTFGNREVLLPKKSGGYYKEYTVPTPGSDDRGERRIIKGAGGELYYTSDHYQSFRRIR
                     EGR"
     gene            2708..3046
                     /locus_tag="PLK64_09090"
     CDS             2708..3046
                     /locus_tag="PLK64_09090"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HPV79943.1"
                     /translation="MLGRRADLHAVIAQAQAAGRSVRLVVGGMDKQSVLTVFADAIGA
                     PDWFGNNYDALTDALREVSDPDGRPIEIIWDGVAVLRSLDKPTYVAVRELLREVDEER
                     SDLTVTIVDR"
     gene            3188..4327
                     /locus_tag="PLK64_09095"
     CDS             3188..4327
                     /locus_tag="PLK64_09095"
                     /inference="COORDINATES: protein motif:HMM:NF023043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HPV79944.1"
                     /translation="MTYQPGDVANGYVLGEDYQWHPLPPAETAPAAPVEPAPTSDPVA
                     PPQWVAPADPITSSDPVASTGPIAASDPTAPAYSPVPYSPVGEPTSAPPALPTPPTTA
                     LPQTAPQGYPQEPAPTYAPQPYQQPGYPGQGYPQQGYQQPGYPQQGHPQQPGYPQQGY
                     AGATPPGPPTKRPFYKKWWFWLIAALLVIAVVVAVVIAALRTFGNAITSVTTVTSTAQ
                     ATPRPTTSSPKPSTTTPQSSTTTTTTPPPATGGSGQPGRDGDLQFTVTGVDCTKNSVG
                     TAPLTMKADGVFCLVSLTIDNSGTKDADLNPFFQEATDSKGDVHFPDLAALVFMDDAD
                     SFTDPVPAGGTAKGILPFDVPAGSTLTSIELHESFNSPGTKIAIP"
     gene            4346..5593
                     /gene="dxr"
                     /locus_tag="PLK64_09100"
     CDS             4346..5593
                     /gene="dxr"
                     /locus_tag="PLK64_09100"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPV79945.1"
                     /translation="MSSDLGPCRTVTLLGSTGSIGTQAITVIQANPQRYRVVGLSAGG
                     SDLRQIAAQAVEQQAEVVAVARGAIDDVRAAIAAAAAEAGDPAYQPEVLVGPDASREL
                     AARPVDVVLNGMTGAIGLEPTLAALRAGSTLALANKESLIIGGPLVKALAAPGQLVPV
                     DSEHSAIAQCLRGGRASEVRRLVLTASGGPFRGMPRADLHDVTPEQALAHPNFSMGRV
                     VTTNSATLVNKGLEVIEAHLLFDVPFERIAVVVHPQQQIHSMVEFQDGSTMAQVGPPR
                     MLVPIALGLSWPDRLPNVDVPTDWSVAQSWQFLPLDDEAFPAVALARRAGSTGGTGPA
                     VYNAANEVCVDAFHDGAIRFTDIVDTVARVLDRHLAGDAQEVCRAGTGSDSPDLDVAG
                     VLRADRWARGEARRVLGLSEEQL"
     gene            5590..6939
                     /locus_tag="PLK64_09105"
     CDS             5590..6939
                     /locus_tag="PLK64_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPV79946.1"
                     /translation="MTTVYYIAGILLIVLGVGVSIALHELGHLVPAKRFGVKCSQYMI
                     GFGPTLWSRTIGDTQVGVKAIPLGGYVRMIGMIPPRAGDAPGQLRSMSTSRMGTLVDQ
                     ARQQSMDEIEPGDENRVFYKLSVPKKVIVMLGGPLMNLALAFVMLTILMSGIGVQSLV
                     PTVSTVAQCIPQTPPTVAKPFADCVAGDPKAPSVAAGLQAGDTIVEVGGVPVQLWAEA
                     TAKIRASAGQPLDLMVERTGTRVPLTVQVGQVVRPTFTEEGKVAVRADGTVITETVGY
                     LGASASVGYVRQPLSTVPGELGRMLGATASVLLRIPEKMVGVFEAVTGQSDRDPNGPV
                     SVVGVARAGGEIVSGQYGPETGQSIAYRIIGLIFGLNLALFLFNLVPLLPLDGGQVVG
                     ALWEGVKKTWARLRGRPDPGYVDVAKALPIAYAVSTVLIAMTLLLAYADLVTPIKLG"
     gene            complement(7015..7653)
                     /locus_tag="PLK64_09110"
     CDS             complement(7015..7653)
                     /locus_tag="PLK64_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03085 family metal-binding protein"
                     /protein_id="HPV79947.1"
                     /translation="MTRYAQLERHALADTMLAVGPDAPTLCDPWRVRDLAAHLIIRET
                     RPDLAAGMFVPRLGARLERAQDELAATDFAELVERVRSGPPRWSAAGVPAVDEAMNRM
                     EFFVHHEDVLRGDPTTPRRDLDPGLAKAVWSGLIRTGGLLYRRAPVGVILVADGVGRQ
                     VVRRPSDAGSAVVRGSVAELVMYSYGRKRAADVKITGPQAAVAALTATDIAL"
     gene            7698..>8230
                     /locus_tag="PLK64_09115"
     CDS             7698..>8230
                     /locus_tag="PLK64_09115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV79948.1"
                     /translation="MIELRRPDASLHREWLDFVSDYDRPGVDGGSVRDDELAGLADPG
                     VFAAWVAQLRDQEAGRNIAPDRVPCTSRWIVREGEIVGVINLRHELNDLLLAWGGHIG
                     YAVRTSARRQGIATRALALMLGEAARLGINPVLVTCDEDNVGSRRTIEGAGGVYDGSV
                     EGKRRNWITLADTPIGYA"
BASE COUNT         1300 a         2824 c         2870 g         1236 t
ORIGIN      
        1 cgacgcgcct catgacgatg aagcagccca tcggcccgac cctcatgatc acgccgtgga
       61 acttcccgct ggccatggga acccgcaaga tcgggccggc catcgccgcc ggatgcacca
      121 tggtcgtcaa acccgccgcc cagaccccgc tgacgatgct cctgctcacc caggtgctcc
      181 tcgagtgcgg ggtccccgcc ggggttctca acgtggtgtc gacggcccac accggggagg
      241 tgatggagcc gatcatccgc gacccgcggc tgcgcaagct caccttcacc gggtccaccc
      301 aggtcgggca gcggctggtc gaacagtccg cgcaggggtt gttgcggctg tcgatggaac
      361 tcggtggcaa cgcgccgttc atcgtcttcg acgacgccga cctcgaccgg gccgtcgacg
      421 gcgccatgct ggccaagatg cgcaacatcg gagaagcctg cacggctgcc aaccgcttcc
      481 tcatccacga atcggtcgcc gaggagttcg gccggcggtt cgccgagcgg atggcggcgc
      541 tgcgggtcgg caagggcacc gtcaagggcg tcgacgtagg ccccctggtg gaccgcaagg
      601 cccgcgagag cgtcgatgcc ctcgtgcagg aggccgtggc cggcggtgcc aagatcctca
      661 ctggtggtgg accgctgccg gggaagggct ggttctacgc cccgaccgtc ctcaccgacg
      721 tccccgcgtc gtcccggatc gccgtcgagg agatcttcgg accggtcgcg ccgatctcga
      781 ccttcgcgac cgaggcggag gcgatcgcgc gagccaacga cacggcatac gggctggtcg
      841 cgtatgcctt cacccgcgat ctggcgcggg cgctgcgggt cagcgaagag ttggagttcg
      901 ggatggtcgg catcaaccaa ggtgtcgtgt ccaatccggc ggcgcccttc ggcggcatga
      961 aggccagcgg gtttggccgc gagggcggct tcgagggcat cgatgaatac ctcgaggtca
     1021 aatacgtcgg cattgccccg taacgaggcg gtcccggggt cggatcgggg cccagcatcg
     1081 tccgaacggc tcgacttggc ccccgagtga tgaccgcagc cttcggactt ggtcgaatgg
     1141 agcgtatgag tcagcaaggg ggtcgctcag gcgcgcgaca ccgggggatc acgagcctgg
     1201 ccgaactgct ggcatctgcc gatggtcgga tgcaggacgg tgcgcggatc ggagcctcga
     1261 tctggcccac cggtttggaa ccactcgacg gccaccttgg gggaggtctg cgcggcggtg
     1321 aacttacgct catcggcgga ccacaggggc tcgggaagac gacgatggcg ctgcagatgg
     1381 tgcgcaacac tgttgcggcc gggcgcagcg ccatcgtgtt ctccttcgaa cacgaaggcc
     1441 gcatcctgct tgagcggctc atcgccctgg aagcggccac ggtcgccggt cggggcagcg
     1501 tcacgatccg ccaactgcgg gatgtcttcg aggaagggtc gccgcacggg ggcggcctca
     1561 tcgagcggct cgcgcagcta cctggagcgg cggagggcat cgccgcccag gaggcgtatg
     1621 ccgcgcgcct gcacctgcac gagtccagcg gcagcacgac cgacatggac gagatccggg
     1681 agatcgtcgc tcagctcaag gaggccgggg aggaaccgct cgtcctcgtc gactacctgc
     1741 agaaagtgcc catcctgggt gggaggcacg tcgaggagga acgagtcacc gaggtcgtcg
     1801 agggtctcaa ggaactcgct ctggacgccg atctgcccgt cgtcgccatc gtcgcggcgg
     1861 acaaagcggc gctcgtggcc ggccaccgga tgcgcctgca cgacctgcgc gggtcctcgg
     1921 cgctggccta tgaggccgac atcgccctca tcctcaacgg caagtacgac gtggtggctc
     1981 gtcatcacct gatgttcgac gtggccaacg ccgaacggtt caagaactgg gtcgtcgtca
     2041 ccgtcgagaa gaaccgcagc ggcctggccg acatcagcat ggagttcgag aagcggttcc
     2101 acgaaggtcg gttcgagccc aaggggcgca tggtccagga gcagctcatc gagggccgcg
     2161 tcttcctcga gtgagccacg tcttcctcga gtgagctgtc ggctcgcggc acaatgggcg
     2221 ttgtgacctc gccgatgagc agatcgaccc gccaactgct ccagctcgtc gcggtgggtg
     2281 tcgtcgcgct cgtcctcgtg gcggcatacc tgctggcgcg tggtgggagc gctgggaccg
     2341 ccggcgtggg ggcgcagccc acggcccgcc ccacgacctc gacgcaggcg accggaggca
     2401 cggtcgatcc ggccagcggg ttggtctggg tggacgagtc gacgctgcca gcccaagcca
     2461 aagcgacgct ggcactgatc cgcgcaggcg gaccgtatcc ctatccccgc aacgacgacc
     2521 agaccttcgg caaccgcgag gtgctgctgc cgaagaagtc cggcggctac tacaaggagt
     2581 acaccgtccc gacccccggc tccgatgatc gcggagagcg gaggatcatc aagggcgcgg
     2641 gcggcgagtt gtactacacc tccgaccact accagagttt ccgacggatc cgggagggtc
     2701 gatgaggatg ttgggccgca gggccgacct gcatgcggtg atcgcgcagg cgcaggccgc
     2761 cgggcgcagt gtccgactgg tcgtcggcgg catggacaaa caatcggtgc tcaccgtctt
     2821 cgcggacgcc atcggggcgc cggactggtt cggcaacaac tacgacgcgc tcaccgacgc
     2881 tctgcgcgag gtctccgatc cggacggacg accgatcgag atcatctggg acggggtggc
     2941 cgtgctgcgc agtctcgaca aacccaccta cgtggccgtc cgcgaactgc tccgagaggt
     3001 cgacgaggag cgctctgacc tcacggtgac gatcgtcgat cgctgacctg tggcgctgtc
     3061 gagccgcccg cccgatgtga tcgcccgggg ccggacgccc gcgccgattc cgaggtcgga
     3121 cgataggctg gcagggacgg cccggggtcc cccgcgtgcc tgagtcggca cgctgaaggg
     3181 cacgcgaatg acctaccaac cgggcgacgt cgccaacgga tacgtcctgg gcgaggacta
     3241 ccagtggcat ccgctgccgc ctgccgagac ggctcccgcc gcgccggtcg agccggcccc
     3301 gacgagcgac ccggtcgcgc cgccccagtg ggtcgcgccc gcggacccga tcacctcgag
     3361 tgacccggtc gcctcgaccg gccccatcgc agccagcgac cccactgcgc cggcctattc
     3421 gcccgtgccc tacagcccgg tgggggagcc gacctcagcc ccgccggccc tgccgacccc
     3481 gcccaccaca gcgctgccgc agactgcccc acagggttat ccgcaggagc cggcgccgac
     3541 ctacgcgccg cagccctacc agcagccggg ttaccccggg cagggctacc cccagcaggg
     3601 ttaccagcag ccgggttacc cgcaacaggg ccatccccag caaccgggtt acccccagca
     3661 gggctatgcc ggggcgaccc cgcccggacc cccgacgaag cggccgttct acaagaagtg
     3721 gtggttctgg ctcatcgcgg cgctgctcgt catcgccgtc gtcgtagctg tcgtgatcgc
     3781 ggcgctgcgg acgttcggca acgccatcac gtcggtcacg acggtcacct cgaccgccca
     3841 ggcaacgccc aggccgacca cctccagccc gaagccgtcg accacgaccc cccagtcgag
     3901 cacgacgact acgacgacgc cccctcccgc gaccggcggg tccgggcagc ccggtcggga
     3961 cggtgacctg cagttcacgg tcactggggt ggactgcacg aagaacagcg tcggcaccgc
     4021 gccgctgacc atgaaggccg atggggtgtt ctgcctcgtg tcgttgacca tcgacaactc
     4081 agggaccaag gacgccgacc tcaacccctt cttccaggaa gcgaccgatt cgaagggcga
     4141 cgttcacttc cccgacctcg cggcgctcgt cttcatggac gatgccgaca gtttcaccga
     4201 cccggtgcct gcgggcggga cggccaaggg catcctgccg ttcgacgtgc cggccgggtc
     4261 caccctgacc agcatcgagt tgcacgagtc cttcaactcc ccgggcacga agatcgccat
     4321 cccctgagtg ggagacaatg cccgggtgag ctctgacctc ggaccgtgtc gcaccgtcac
     4381 cctgctgggc tcgacggggt cgatcggcac tcaggccatc acggtcatcc aagcgaaccc
     4441 gcagcgctat cgagtcgtcg ggctatccgc cggagggtcc gacctccgac agatcgccgc
     4501 gcaggccgtc gagcaacagg ccgaggtcgt tgcggtcgcc cggggtgcga ttgacgatgt
     4561 gcgcgctgcg atcgcggcgg cggccgcgga agctggcgac ccggcatacc agccggaggt
     4621 cctcgtcggc cctgacgcga gccgcgagtt ggccgcccgt ccggtcgacg tcgtcctcaa
     4681 cgggatgact ggggcgatcg ggctggagcc caccctcgcg gcgctgcgcg cagggagcac
     4741 gctggccctg gccaacaagg agtcgctcat catcggcggg ccgctggtca aggccctcgc
     4801 ggctcccggg caactggtcc cggtcgacag tgagcacagc gcgatcgctc agtgcctgcg
     4861 tgggggacgt gcgagcgaag tacggcggct cgtcctgacc gcgagcggcg ggccgttccg
     4921 cggtatgccg cgcgccgacc tacacgacgt cacccccgag caggccctcg cccaccccaa
     4981 cttctcgatg gggcgggtgg tgacgacgaa ctcggcgacc ttggtcaaca aggggctaga
     5041 ggtcattgag gcgcatctgc tcttcgacgt gccgttcgag cggatcgccg tcgtcgtcca
     5101 ccctcaacag cagatccatt cgatggtgga gttccaggac gggtcgacga tggctcaggt
     5161 ggggccgccg cggatgctcg tgccgatcgc cctcgggctg tcctggcccg atcggctgcc
     5221 gaatgtcgac gtccccaccg actggagtgt cgcccagtcg tggcagttcc tgccgctcga
     5281 cgacgaggcg ttccccgcgg tggcgctcgc ccgccgcgcg ggcagcacgg gcggcaccgg
     5341 gccggcggtc tacaacgcgg ccaacgaggt gtgcgtcgac gccttccacg acggggcgat
     5401 ccggttcacc gacatcgtcg acacggtcgc gcgggtgctt gaccggcatt tggccgggga
     5461 tgcgcaggag gtatgccgcg ccggcacggg gagtgattca ccggacctgg acgttgcagg
     5521 ggtgttgcgg gccgaccggt gggctcgtgg tgaggctcgg cgggtgctgg ggctcagtga
     5581 ggagcagttg tgacgaccgt ctactacatc gcgggcattc tgctgatcgt gctcggggtc
     5641 ggggtgtcga tcgcgcttca cgaactcggc cacctggtgc cggcgaagcg attcggcgtc
     5701 aaatgctcgc agtacatgat cggcttcggt cccaccctgt ggtcgcggac gatcggtgac
     5761 acccaggtgg gcgtcaaggc gatcccgctc gggggctacg tgcggatgat cggcatgatc
     5821 ccgccgcggg ccggcgacgc gccagggcaa ctgcgcagca tgtcgaccag ccggatgggg
     5881 accttggtcg accaggctcg ccaacagtcg atggacgaga tcgagcccgg cgacgagaac
     5941 cgcgtgttct acaagctgtc ggtgccgaag aaggtcatcg tcatgctcgg cgggccgctg
     6001 atgaacctgg cgctggcctt cgtcatgttg acgatcctca tgtcaggcat cggagtccag
     6061 tcgctcgtcc ccaccgtctc taccgtcgcc cagtgcatcc cgcagacgcc gccgacggtc
     6121 gccaagccgt tcgccgactg cgtggccggc gacccgaagg cgccctcggt ggctgctggg
     6181 ctgcaggcgg gcgacaccat cgtcgaggtg ggcggtgtgc ccgttcagtt gtgggctgag
     6241 gccaccgcga agatccgggc ttcggcgggt cagcccctgg acctcatggt cgagcgcacc
     6301 ggcacccggg tacccctgac cgtccaggtc gggcaggtcg tgcggcccac gttcaccgaa
     6361 gaagggaaag tcgccgtccg cgccgacggc acggtcatca ccgagaccgt cggctatctc
     6421 ggggcttcgg cgagcgtggg ctacgtccgt cagcccctca gtacggtgcc gggggaactc
     6481 ggccggatgc tcggcgccac cgcctcggtg ttgctgcgaa ttcccgagaa gatggtcgga
     6541 gtgttcgagg ccgtcaccgg gcagtccgac cgcgatccca acggccctgt gtcggtcgtc
     6601 ggggtcgccc gcgccggcgg agagatcgtc agcggccagt acgggccgga gaccggccaa
     6661 tcgatcgcct accggatcat cgggctgatc ttcgggctca accttgcgct gttcctcttc
     6721 aacctggtcc cgctgctgcc gttggatggc ggtcaggtcg tcggcgcgtt gtgggagggg
     6781 gtcaagaaga cttgggctcg gctacgtggt cggcccgacc ccgggtacgt cgacgtcgcc
     6841 aaggcactgc ccatcgcgta tgccgtgtcg accgttctca tcgccatgac cctgctgctg
     6901 gcctatgccg acctggtgac gccgatcaaa ctgggttagt cggctgacgc ggcatacgtg
     6961 cgccgtgcgg ctgtgcggca tacgtgcgct gtgcggccgt cccgcagtgc gctgtcagag
     7021 cgcgatgtcg gtcgctgtga gggcggcgac cgcggcctgc gggccggtga tcttgacgtc
     7081 ggcggcccgc ttgcggccgt aggagtacat gacgagctcc gcgactgagc cccgcacgac
     7141 ggcggagccg gcatcgctgg gccgacgcac gacctgacgg cccactccgt cggccacgag
     7201 gatcacgccc accggcgccc gccgatagag caacccgccc gtgcggatca ggcccgacca
     7261 gacggccttg gccaggcccg ggtcgaggtc ccggcggggt gtggtggggt caccacgaag
     7321 gacatcctcg tggtggacga aaaactccat ccggttcatc gcctcgtcga cggccggcac
     7381 cccagcggcc gaccaccgcg gcgggccgga gcggacccgc tcgacgagct cggcgaagtc
     7441 ggtcgcagcg agctcgtcct gggcacgttc gagccgagcc cccagccgcg ggacgaacat
     7501 ccccgcggcc aggtcgggcc gggtctcgcg aatgatgagg tgcgcggcca ggtcgcggac
     7561 ccgccagggg tcacacaggg tgggggcgtc cggaccgacg gcgagcatcg tgtcggcaag
     7621 ggcgtggcgt tccagttgcg catagcgggt cacaaaacgg atcgtgtcac acccggggcc
     7681 cgacctaagg tggcgaaatg atcgaactgc gccgccctga cgcgagtctg caccgagagt
     7741 ggctcgactt cgtgtccgac tacgaccgcc cgggcgtcga cggcggcagc gtcagggacg
     7801 acgagttggc cgggctggcc gatccgggtg tgttcgcggc ctgggtggcg caactgcgcg
     7861 accaagaggc gggccgcaac atcgcgcccg accgagtccc ctgcacgagc cggtggatcg
     7921 tccgcgaggg cgagatcgtc ggggtgatca acctgcgcca cgaactcaac gacctccttc
     7981 tcgcctgggg cgggcacatc gggtatgccg tgcgcacctc ggcccgtcga cagggcatcg
     8041 cgacccgcgc cctggccctc atgctcggcg aggccgcccg gttggggatc aaccccgtcc
     8101 tagtcacctg cgacgaagac aacgtcgggt cgcggcgcac catcgaaggc gcgggaggtg
     8161 tctacgacgg gtcggtcgag ggcaagcgac gcaactggat caccctggcg gacaccccca
     8221 tcgggtatgc
//
LOCUS       DAOMZY010000140         8205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_8241_length_8205_cov_6.006012, whole genome shotgun sequence.
ACCESSION   DAOMZY010000140 DAOMZY010000000
VERSION     DAOMZY010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 8205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8205
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8241_length_8205_cov_6.006012"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="PLK64_09120"
     CDS             complement(<1..635)
                     /locus_tag="PLK64_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HPV79949.1"
                     /translation="MIVDLPSTSTAAIGKQLVQLRNEVGALALGRVLTLIVVVDDDAA
                     DEAVEIATEASRQHPCRIIVVISGSRRGANRIDGQIRVGGDAGASEVVIMRLFGKLAE
                     HGQSVVTPLLLPDSPVVAWWPREAPSDLRHDPIAAMATRRVTDSAECSHPRAMLTRLA
                     ETYQPGDTDLSWTRITLWRGLLAAALDGPPYESVTESRGLPPPERTRKPAAV"
     gene            complement(632..2173)
                     /gene="zwf"
                     /locus_tag="PLK64_09125"
     CDS             complement(632..2173)
                     /gene="zwf"
                     /locus_tag="PLK64_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HPV79950.1"
                     /translation="MSPARVSAGVNPLRDRRDRRLPRIAGPCGLVLFGVTGDLARKKL
                     MPAIYDLANRGLLPPGFSLVGFARRDWADQDFGQIVHDSVRDHARTPFREDVWKSLAE
                     GIRFVPGTFDDDAAFDTLAATVHDLERSRGTGGNLAFYLSIPPGFFAQVCQQLDRSGL
                     AHSDGQSWRRVVIEKPFGHDLASARELNAIVESVFPADSVFRIDHYLGKETVQNLLAL
                     RFANQLFEPVWNANYVDHVQITMAEDVGIGGRAGYYDGIGAARDVIQNHLLQLLALTA
                     MEEPVSFDAASLRAEKEKVLSAVVVPAKIGEGTARGQYAAGWQGGELVPGYLEEEGVR
                     TGSTTETYAAVRLEIATRRWAGVPFYLRTGKRLGKRVTEIAVVFKKAPHLPFDTTATE
                     ELGQNAVVIRVQPDEGITMRFGAKVPGAQMEVRDVTMDFGYGSAFTESSPEAYERLIL
                     DVLLGEPPLFPRHEEVELSWQILDPIEAWWRRHGKPDPYPSGGWGPASSDRMLARDGR
                     VWRKP"
     gene            complement(2246..3361)
                     /gene="tal"
                     /locus_tag="PLK64_09130"
     CDS             complement(2246..3361)
                     /gene="tal"
                     /locus_tag="PLK64_09130"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HPV79951.1"
                     /translation="MTAASTDNLADLAAAGVSIWLDDLSRSRLTSGSLAALIRDQHVS
                     GVTTNPSIFQAAITSGTDYDEQLASLAAAGADADAALFAITTDDVRAGCDVFASVAAA
                     TDGVDGRVSIEVDPRLAHQTGPTIEQAKALAAAVGRDNVYIKIPATLEGLPAIAAVLA
                     EGISVNVTLIFGLQRYRAVMNAFVEGLERAHEAGHDLRTIHSVASFFVSRVDTEIDGR
                     LEAIGTDAARALKGQAAVANARLAYQAFEEVFATPRWRSLADEGARRQRPLWASTGVK
                     DPAYPDTLYVVDLVAADCVNTMPEKTLRAVADHGTVRGDTITGEYAAAQGVLDAVEAQ
                     GVSYIEVVEVLEREGVEKFEKAWVSLLADVTGALARA"
     gene            complement(3358..5517)
                     /gene="tkt"
                     /locus_tag="PLK64_09135"
     CDS             complement(3358..5517)
                     /gene="tkt"
                     /locus_tag="PLK64_09135"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPV79952.1"
                     /translation="MAGRDSHVPRPTATAVGWTDLDIRAVDTARLLAADAVQKVGNGH
                     PGTAISLAPAAYLLYQHVMTHDPSDPHWLGRDRFVLSCGHSSLTQYVQLFLSGYGVEL
                     SDLQSLRTWGSLTPGHPEVNHTPGVEITTGPLGSGLASAVGMAMAQRRQRGLLDPEAA
                     AGESIFDHHIYVLASDGDIMEGVTHEACALAGHQELGNLTVIYDQNQISIEDDTDISF
                     SEDVAARYVAYGWDVRTVDWRGATADGATYVEDIDALNAAIEAGRAITDKPSLIVLRT
                     IIAWPSPTKQNTGKSHGSALGADEIAGLKTALGFDPEVAFPVAPRVLTQARKVVARGK
                     AAHKAWDRRYAAWRKAQPERAALLDRIVERGLPTDLAAALPSWPADAKGIATRAASGK
                     VLSALAPVLPELWGGSADLAESNNTTMAGEPSFIPARRQTHEWKGGPYGRTLHFGIRE
                     FGMGAILNGIALEGLTRPYGGTFLVFSDYMRGAVRLAAIQQLPVTFVWTHDSIGLGED
                     GPTHQPIEHLAALRAMPGLDVVRPADANETAYAWLEILRNARPAALALSRQNLPVLDR
                     KVHAPARNVAKGGYVLIDGGADGRQAAEVILVATGSEVALALAAREQLERARIRTRVV
                     SMPCREWFDQQSKSYRDRVLPPTVRARVSVEAGVAQGWRDIVGDAGRIVSIEHFGASA
                     DAATLFREFGFTPEAIVAAAKASLKTARAGARKEGLT"
     gene            5657..6622
                     /locus_tag="PLK64_09140"
     CDS             5657..6622
                     /locus_tag="PLK64_09140"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HPV79953.1"
                     /translation="MTTVDPRAELASHPGSTGRGFGFVVGQYVALTKPRIIELLLITT
                     VPVMVLAERGIPSGWLVLATLVGGTLSAGAANTLNCVIDRDIDALMNRTASRPLVTGT
                     ISTRAAVMFGFTLCVVATLVLGVFVNWLSAWLALAAFAFYVGIYSVVLKRRTPQNIVW
                     GGAAGCMPVLIGWSAVTNRVDWPAWILFLVIFFWTPPHYWPLSMKFKDDYAAASVPML
                     PVVARMVVVSRQIVIYSWVMVLVSLTLIPVAPMGWVYAVGAAVSGGLFIAEAHRLHHQ
                     ALAGAPQAQLRPMRLFHYSITYLTVLFVAVAVDPLLYLAIPGLAR"
     gene            6692..7216
                     /locus_tag="PLK64_09145"
     CDS             6692..7216
                     /locus_tag="PLK64_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPV79954.1"
                     /translation="MTSRTLSRPQFTAAALALGTALTLSACGGSATPLAAPAASTAMS
                     DKTAMSDKTSMPDKTAMADKTGAPAAMAKGAYVSYADYQSSMAMYQGSKVVLFFHASW
                     CPDCRATDTALTGSAVPDGLTVVKVDYDTATELKQKYGITQQHTFVQVDPAGMSVKKW
                     TGTKDGAAIKAQTT"
     gene            7216..8016
                     /locus_tag="PLK64_09150"
     CDS             7216..8016
                     /locus_tag="PLK64_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HPV79955.1"
                     /translation="MLALLGALVAGALTTLAPCVLPLLPVIVGGAAASDLAGSAAGST
                     AGGAASPTVRTQVRWVTRNRRALVITASLGASITLFTLILKATTALLVIDPRTWSILS
                     GVILVLLGIAGAFPDLWERLSATLSLQGRSDARLRAARARGGLTGDILTGAALGPVFS
                     SCSPLYAYVVVTVLPARFGYALTLLAAYVLGLCVILLAIALLGRRLIARLGWAADPHG
                     RFRRILGLVFILVGIGIALGWDRSLQTWILEHSPIAPWELDSGFIPEG"
BASE COUNT         1186 a         2889 c         2833 g         1297 t
ORIGIN      
        1 accgctgccg gcttgcgtgt cctctcgggt ggcgggagcc cccgactctc cgtcaccgac
       61 tcgtagggcg gcccgtcgag ggccgccgcc agcaggcccc gccacagggt gatccgggtc
      121 cacgacaggt cggtgtcgcc gggctggtag gtctcggcga ggcgggtcaa catcgcccgt
      181 gggtgagagc actccgccga gtcggtcacc cgccgggtgg ccattgccgc gatggggtca
      241 tggcgcaggt cgctgggtgc ttccctcggc caccaggcca cgacggggga atcggggagc
      301 aacagcgggg tgacgacgct ctgaccatgc tcggcgagtt tgccgaacag ccgcatgatg
      361 acgacttcgc tggcaccggc gtcaccaccg acccggatct gaccgtcgat gcggttggct
      421 ccccgccggg agccactgat gacgacgatg atgcggcagg ggtgctggcg agaggcctcg
      481 gtggcgatct cgaccgcctc gtcggcagca tcgtcatcga cgacgacgat gagggtgagg
      541 acccggccca gggccagggc cccgacctcg ttgcgcagct ggaccaactg cttgccgatg
      601 gccgcggtcg aggtcgaggg gaggtcgacg atcatggctt cctccatacc cggccgtcgc
      661 gggccagcat ccggtcgctc gacgccggtc cccatccgcc cgacggatac gggtccggct
      721 tgccgtgccg ccgccaccag gcctcgatcg ggtcgagaat ctgccaggac aactcgacct
      781 cctcatgacg ggggaacagg ggtggttcgc ccagcaagac atcaaggatg agtcgctcat
      841 aagcctcggg ggagctctcg gtgaaggcac tgccgtaacc gaagtccatc gtcacgtcgc
      901 gcacctccat ctgggctccg gggaccttgg cgccgaaccg catggtgatg ccctcgtcgg
      961 gctgcacccg gatgacgacg gcgttctggc ccaactcctc ggtggcggtc gtgtcgaacg
     1021 gcaggtgcgg cgccttcttg aagacgaccg cgatctcggt gacccgcttg cccagccgtt
     1081 tcccggtgcg caggtagaac gggacgcccg cccatcgacg ggtcgcgatc tcgaggcgga
     1141 ccgcggcata cgtctcggtg gtggacccgg tgcggacccc ttcctcctcc aaatagccgg
     1201 ggaccaactc gccaccctgc cagccggcgg catactgacc gcgcgccgtc ccttccccga
     1261 tcttggcggg gaccacgaca gccgagagca ccttctcctt ctcggcgcgc aggctggccg
     1321 catcgaagga gaccggctcc tccatcgcgg tcagggcaag cagttgcagc aggtggttct
     1381 ggatgacgtc gcgggccgcg ccgatgccgt cgtagtagcc ggcccgcccg ccgatgccga
     1441 cgtcctcggc catggtgatc tgcacatggt cgacgtagtt ggcgttccag accggctcga
     1501 acaactggtt ggcgaagcgc agcgccagga ggttctgcac cgtctccttg ccgagatagt
     1561 ggtcgatccg gaagaccgag tcggccggga agaccgactc gacgatcgcg ttgagctcac
     1621 gggccgacgc gagatcgtgc ccgaacggct tctcgatgac cacccgacgc cacgattgac
     1681 cgtccgagtg ggccagcccc gagcgatcca attgttggca cacctgggcg aagaaccccg
     1741 gcgggatcga caggtagaag gcgaggttgc cgccggtgcc gcgagagcgc tccaggtcgt
     1801 gcacggtggc agccagcgtg tcgaaggccg cgtcgtcgtc gaaggtcccc ggcacgaagc
     1861 ggatgccctc ggcgagggac ttccagacgt cctcacggaa gggcgtgcgg gcgtggtcgc
     1921 gcaccgagtc gtggacgatc tggccgaagt cctgatcggc ccagtcccgc cgagcgaacc
     1981 cgaccaggct gaaacccgga ggcagcaggc cgcggttggc caggtcgtag atggccggca
     2041 tgagcttctt gcgagccagg tcgccggtca ccccgaagag caccaggcca cacggcccgg
     2101 cgatcctcgg gagccgtcga tccctgcgat cgcgcaacgg gttgactccg gcagacacgc
     2161 gggcgggact catgggcgag aacctacctg cgcggggcgc ggggacgcgg catacgtcaa
     2221 cccaggtgta tgccgcgttg ccaggtcacg cacgggcgag ggcgccggtg acgtcggcga
     2281 ggagggagac ccacgccttc tcgaacttct cgacgccttc gcgttcgagc acttcgacga
     2341 cctcgatgta ggagacgccc tgcgcctcga ccgcatcgag gacgccttgg gcggcggcat
     2401 actccccggt gatcgtatcg ccacgcacgg tgccgtgatc ggcgacagcg cgcagggtct
     2461 tctccggcat ggtgttgacg cagtcggcgg cgacgaggtc gacgacgtag agcgtgtcgg
     2521 gatatgcggg gtccttgacg ccggtggacg cccacagcgg ccgctgccgc cgggcgccct
     2581 cgtcggccag ggagcgccac cgcggggtcg cgaagacctc ctcgaaggcc tgataggcca
     2641 accgagcgtt ggcgaccgcg gcctggccct tgagggcccg cgcggcgtcg gtgccgatgg
     2701 cctccagccg accgtcgatc tcggtgtcga cccgcgacac gaagaacgac gcgaccgagt
     2761 ggatcgtgcg caggtcgtgg cccgcctcgt gagcgcgctc gagtccctcg acgaaggcgt
     2821 tcatcaccgc ccgatagcgc tggaggccaa agatcagcgt gacgttgacg ctgatgcctt
     2881 cggccaacac cgcggcaatc gccggcagcc cctcgagggt ggccgggatc ttgatgtaga
     2941 cgttgtcgcg accgaccgcc gcagcgaggg cctttgcctg ttcgatggtg ggcccggtct
     3001 ggtgggccag cctcgggtcg acctcgatgg agacccgacc gtcaacgccg tcggtggccg
     3061 cggccaccga ggcgaacacg tcgcagccgg cgcggacgtc gtcggtggtg atggcgaaga
     3121 gcgccgcatc ggcgtcggcc ccggccgcag ccaagctcgc caactgctcg tcgtaatcgg
     3181 tgcccgaagt gatggctgcc tggaagatgg acgggttggt cgtcaccccg ctgacgtgct
     3241 ggtcccggat gagggcggcc agactgccgg aggtgagtcg gctgcgggac aggtcatcca
     3301 gccagatcga gacaccggcc gccgcgaggt cggccaggtt gtcggtgctc gctgcggtca
     3361 tgtcaggcct tccttccgcg caccggcccg ggcggtcttg agcgaggcct tggccgcggc
     3421 gacgatcgcc tcgggggtga acccgaactc ccggaagagc gtcgcggcat cggccgacgc
     3481 accgaagtgc tcgatcgaca cgatccgccc ggcatcgccg acgatgtccc gccacccttg
     3541 ggcgaccccc gcctcgacgg acacgcgggc ccgcacggtc ggcggcagga cccggtcgcg
     3601 atagctcttg gactgctggt cgaaccattc acgacagggc atcgagacga cccgggtgcg
     3661 gatccgggcc cgctcgagct gctctcgggc ggccagggcc agggcgacct cggagccggt
     3721 ggccacgagg atcacctcgg cggcctgccg gccatcggcc ccgccgtcga tgaggacgta
     3781 gccacccttg gcgacattgc gggcgggcgc gtggaccttg cggtccagga cgggcaggtt
     3841 ctgacgggac agcgccagcg ccgctggccg ggcgttgcgc aggatctcca accaggcgta
     3901 tgccgtctcg ttggcgtccg cgggccgcac gacgtcgagc cccggcatcg cgcgcagagc
     3961 cgccaggtgc tcgatgggct ggtgggtcgg gccgtcctcg cccaggccga tcgagtcgtg
     4021 cgtccagacg aaggtcaccg ggagctgctg gatcgcggcc aggcgcacgg caccgcgcat
     4081 gtagtcggag aagacgagga aggtgccgcc atagggccgg gtgaggccct ccaaggcgat
     4141 gccgttgaga atcgcgccca tcccgaactc acggatgccg aagtgcaggg tgcgcccata
     4201 ggggccgccc ttccactcgt gcgtctggcg tcgggccggg atgaacgacg gctcgccggc
     4261 catcgtcgtg ttgttcgatt cggccaggtc ggccgacccg ccccacaact cgggcaggac
     4321 gggggccagg gcagagagca ccttgccgga cgcggcgcgg gtggcgatgc ccttggcgtc
     4381 ggccggccag ctcggcaggg ccgcggccag atcggttggc aggccccgct cgacgatccg
     4441 gtccagcagc gcggcgcgct cgggctgggc cttgcgccac gcggcatacc tcctgtccca
     4501 ggccttgtgc gcggccttgc cgcgggcgac gaccttgcgc gcctgggtga ggacgcgcgg
     4561 ggcgaccggg aaggcgacct ccgggtcgaa gccgagagcg gtcttgagcc cagcgatctc
     4621 gtcggcgccc agggccgagc cgtgcgactt gccggtgttc tgcttggtgg gggagggcca
     4681 ggcgatgatc gtgcgcagaa cgatgaggct cggcttgtcg gtgatcgcgc ggccggcctc
     4741 gatggctgcg ttgagggcgt cgatgtcctc gacataggtg gccccgtcgg cggtcgcgcc
     4801 ccgccagtcg accgtgcgga cgtcccagcc gtaggcgaca taacgcgccg cgacgtcctc
     4861 gctgaacgag atgtccgtgt cgtcctcgat ggagatctgg ttctggtcgt agatgaccgt
     4921 gaggttgccc aactcctggt ggccggccag cgcgcacgcc tcgtgggtga cgccttccat
     4981 gatgtcgccg tcggaggcca ggacatagat gtggtggtcg aagatgctct cgcccgcagc
     5041 cgcctccggg tcgagcaacc cgcgctggcg gcgttgcgcc atcgccattc cgactgcgct
     5101 ggccaggccc gaacccaacg ggccggtggt gatctcgacg ccgggggtgt ggttgacctc
     5161 cggatgccca ggtgtgaggc tgccccaggt gcgcagggac tgcaggtccg agagctccac
     5221 gccgtagcca gacaggaaca actggacgta ctgcgtgagg cttgagtgcc cgcaggacag
     5281 gacgaaccgg tcgcgtccca gccagtgcgg atcgctcgga tcgtgggtca tcacgtgctg
     5341 atagagcagg tatgccgcgg gggccaggga gatcgccgtg cccggatgcc cgttgccgac
     5401 cttctgcacc gcatcggccg ccaacagccg ggccgtgtcg acggctcgga tgtccagatc
     5461 ggtccacccg accgccgtcg ccgtcgggcg gggcacgtgg gagtcgcgac ctgccaagag
     5521 cttggaaggg gacgaggcgg atgtcacgga ggtgttctcc ttcacactgg acgggtcacg
     5581 tgcagcctaa tccagtgggg atcgacacct cggccccgtg ccagcaggcg tcacgccgcc
     5641 ggtagactcc ccgactgtga ccaccgtcga cccgcgcgcc gagctcgcct cgcacccggg
     5701 gtccacgggc cgcggcttcg gcttcgtcgt cggccagtac gtcgccctca ccaagccccg
     5761 gatcattgag ctgctgctca tcacgacggt ccccgtcatg gtcctagccg agcgcggcat
     5821 accgtcgggg tggttggtcc tcgccaccct ggtcggtggg acgttgtccg cgggcgccgc
     5881 caacacgctc aactgcgtca tcgaccgcga catcgacgcg ctgatgaacc gcaccgccag
     5941 ccgccccctg gtcaccggga ccatctcgac ccgcgccgcc gtgatgttcg ggttcacgct
     6001 gtgcgtcgtc gcgaccctcg tgctcggagt cttcgtcaac tggctctcgg cctggctggc
     6061 cctcgccgcc ttcgcgttct acgtgggcat ctactcggtc gtcctcaagc gccgcacccc
     6121 gcagaacatc gtctggggcg gtgccgcagg gtgtatgccg gtgctcatcg gctggtcggc
     6181 cgtcaccaac cgggtcgact ggccggcgtg gatcctcttc ctcgttatct tcttctggac
     6241 gccgccgcac tactggccgc tgtcgatgaa gttcaaggac gactacgcgg ccgcatctgt
     6301 gccgatgctg ccggtggtgg cgcgaatggt cgtcgtctcg cgccagatcg tcatctactc
     6361 gtgggtgatg gtgctcgtgt cgctcaccct catccccgtg gcccccatgg gctgggtgta
     6421 tgccgtgggc gctgcggtct ccggcggcct gttcatcgcc gaggcgcacc gactgcacca
     6481 ccaggctctg gccggtgcgc cgcaggccca actgcgcccc atgcgcctgt tccactactc
     6541 gatcacgtat ctcacggtcc tgttcgtcgc ggtcgccgtc gacccactgc tctacctcgc
     6601 gatccccggt ctggcccgct gacgtcgggc gtcaccgccc cgtaaggtct gcgcaatggc
     6661 caccgcgggg gtatgccgtg aggctggtcc catgacttca cgcaccctct cccgccccca
     6721 gttcaccgct gcagcgctgg ccctcgggac cgccctcacc ctctcggcct gcggcggtag
     6781 tgccaccccg ttggccgctc cggccgccag cacggccatg tcagacaaga ccgcgatgtc
     6841 cgacaagacg tcgatgcctg acaagaccgc gatggccgac aagaccggcg cccccgcggc
     6901 catggccaag ggtgcctacg tcagctacgc cgactaccag tcgtcgatgg cgatgtacca
     6961 gggctccaag gtcgtcctgt tcttccacgc gtcgtggtgc cccgactgtc gcgccaccga
     7021 cacggccctg accggctcgg cggtgcccga cgggctgacc gtcgtcaagg tcgactacga
     7081 caccgcgacc gagctcaagc agaagtacgg catcactcag cagcacacct tcgtccaggt
     7141 cgatccggcc ggaatgtcgg tcaagaagtg gaccggcacc aaggacggcg cagcgatcaa
     7201 ggcgcagacc acctgatgct cgcgctcctc ggggcactgg tcgccggtgc cctcacgacg
     7261 cttgcgccct gcgtcctgcc gctcctcccc gtcatcgtgg gaggggcggc agcctcggat
     7321 ctggccggca gcgccgccgg aagcaccgcc gggggagccg cgtcgcctac cgtgcgcacc
     7381 caggtccgtt gggtcacccg caaccgccgc gctctggtca tcacggcctc gctcggcgcg
     7441 tcgatcacgc tcttcaccct catcctcaag gccacgacgg cgctgctcgt catcgatccg
     7501 cgcacctggt cgatcctctc tggggtgatc ctcgtgctcc tcgggatcgc gggtgccttc
     7561 cctgacctgt gggagcgcct gtccgccacc ctcagcctgc aagggcgctc ggatgcccgg
     7621 ctccgcgccg cccgggcccg tgggggtttg accggcgaca tcctcacggg ggctgcgctc
     7681 ggcccggtgt tctcctcgtg cagcccgctg tacgcctacg tcgtcgtcac cgtcctgccc
     7741 gctcggttcg gctacgcgtt gacgctgctc gcggcatacg tgctcgggct ctgcgtcatc
     7801 ctgctggcaa tcgcgctcct cggtcgccgc ctcatcgcgc gactcggctg ggcggccgac
     7861 ccgcacggcc ggttccggcg catcctcggc ctggtcttca tcctcgtggg gatcggcatt
     7921 gccctcggtt gggaccgctc actgcagacc tggatcctcg aacactcgcc gatcgccccc
     7981 tgggagttgg actccgggtt catccccgag ggctgagcag cgcccggcgg tcagtcgcgc
     8041 gggaggacga cctcgatcgc gcacccagcc gggacgtcgg cgacacccac cgtgccgccg
     8101 tgggcggaca cgatcccctg gacgacggcc aggccaagac ccgctccgtc gccggcgcgc
     8161 ggggtccggg cgtcgtcccc gcgccaccac ggctcgaaca tccgc
//
LOCUS       DAOMZY010000141         8039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_8454_length_8039_cov_4.084544, whole genome shotgun sequence.
ACCESSION   DAOMZY010000141 DAOMZY010000000
VERSION     DAOMZY010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8454_length_8039_cov_4.084544"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="PLK64_09155"
     CDS             <1..1021
                     /locus_tag="PLK64_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79956.1"
                     /translation="GEHVTTLGVQSQPATYLGDLRHLTTLLLHLGHQPGADRLAPWVP
                     LLTASTSDRGGQRGPRWGLRPPTTAGLRAQTLSTADAMLAASDLDAAVEAFQPWVELT
                     PNTPDGVLGWLADRTVMTPTLTRLVMAARAPQRRLSHHLDTPRTEGVRMPINLLAIPQ
                     VIPHEQYVEHLTGVFDSSEVTVRLFASLSLARLHPDVTSWAAAAEALAMPGPIGVRCA
                     RACSATMLVAADEWEARTRRAIEEAPRENYRALEAKIRHSLGMSRWYDEWARRYRPGT
                     RHVSYDLGLSWLWVHVAHGHLDLSPAWRGREPSSKDRAPYRKFESSLNQEQQVELANA
                     VYKRA"
     gene            1219..2193
                     /locus_tag="PLK64_09160"
     CDS             1219..2193
                     /locus_tag="PLK64_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPV79957.1"
                     /translation="MAGRHLKEIFRAFHARDELAFRRAAMEIIEEEQAKHHNALARDL
                     QKLLVAGGGSLDPGSTIVVPAPPKDREGEWDLGEVREPDRLLDELVLSEAVIPVLESL
                     IEEVRHWPALDAAGLPRRQRLLLQGPPGCGKTTAAEAIAAELGRPLLIVRLDAVVSSY
                     LGETASNLRRILEYADQAPFVVLFDEFDALGRSRDDASEHGEMKRVVTAFLQMTDRYR
                     GPSLLLAATNHPALLDEALWRRFDEVLTFGLPTVHQLRQVLRLRLKGVAHSGLEIPRY
                     ASALKGRPHAAAEKLIIDARRNALLRDSAVVTGQDVDRVLPGITARPW"
     gene            2198..4537
                     /locus_tag="PLK64_09165"
     CDS             2198..4537
                     /locus_tag="PLK64_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPV79958.1"
                     /translation="MPEHLLLPNPRPLQSRRSSPAGGGSPNRPHGQHGGHLRQQLRTA
                     RAPRQLNAGIDPDLVFKIKAGSRPEDSAFEGRGLQVLGESIDYTYFVLASDQGASLEQ
                     AIEQYVRTGELRTFFNQIDDIEPYGPGDRTGPGIADLDGSTSGQHTVDVIIWPSGTYV
                     EAQRRASIVEQIVGENGGQTVLRSVSPRRSYLRVSVSSVGLDDLLNTSVVELVRTPPV
                     PFLDFRDWRNVDISTISRSEVASEVVGVLDDSPESAHPLLRGLVMSDESLAPPTYQWQ
                     QRGTHGTEVVGRVLFPALHEELRDAVPISAIGTVRVVRILEPDPSRPDGAPRFATYAP
                     PHLLVEEAIRHLHGTHGVRIFNLSIGYSEPFNDLHLGPLTETIDDLVRELGLVVVLPT
                     GNAPANLSARTPSGHHIIADKPEFFFAPEHRLSEPGPAALAVTVGALALSGAPAELPG
                     RVGWQAAADADEAAPFSRTGPGLGTNSRRANKPDVTHYGGNLVVSDTGNVVHNDLGAS
                     LISTSTRGSGGQVFATVNGTSYAAPAVARVAADIAYAYPDASGNLVRALLAASSAPTS
                     PAATLAELHRRNRVYGMGLPDVDRATSSDARRVTMTYDGAMPVDTVQIHPLPIPEAFR
                     LGSRRDRVISIALAFDPPVRRQRREYLAGTMKFNVYRDISPDELAEILQRQDPDDPAD
                     LVNDRRRLNLQPGVNTFTHSTLQLRTWVGRNSFVNDDETFLIAVTHKAQTWARSDPAY
                     EHQGYALAATLEDRELATADLHQILRQQLRLPTRVRLRA"
     gene            4860..5834
                     /locus_tag="PLK64_09170"
     CDS             4860..5834
                     /locus_tag="PLK64_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79959.1"
                     /translation="MPSALFGVGAAAGAVGSGLGAMGARDLVAAHERQVAAREQYEAR
                     AAASEDLRGVANQRLIEFSALQRAAFTGVVQRMVAWLRSHAKQVRESDRLLVDGLEVS
                     LSLMPSTVGREEAVAGWLTGASNTALAGLGTAGAVTTAVEKYGVAGTGARVSDLSGAA
                     KQKAAAAFRGGGPLKNGGGGIALGNLATKTAVGGISLLAAGSTCKIEGAKALTRAEEF
                     ATHIQVACAEFDVKDAKLRAVVQRVEELTDVLTRLEGQATVAMDRLEALTFDPIEHIS
                     ALSETIELVRGVQGVAQARLINERGDLSKQSEILRVRYREMNGDSSDG"
     gene            5827..6252
                     /locus_tag="PLK64_09175"
     CDS             5827..6252
                     /locus_tag="PLK64_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79960.1"
                     /translation="MADAKPIQGKVMDIKTVVAAPEAFDALTRLVDTAGKYVAIHAEE
                     SRKREALRTYRDTEIATIKAAEVTLQRYFDEAFRERREVFQSLFRAMDHALETGDTQG
                     LSTVVSGIVDVAKDSPLAAAGDLAKLRAAFDDPNQVWSF"
     gene            complement(6538..6726)
                     /locus_tag="PLK64_09180"
     CDS             complement(6538..6726)
                     /locus_tag="PLK64_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79961.1"
                     /translation="MGAACAAAAICDILVLATATHWALEQPETTPWMPSMVAVTASLL
                     RLTLTQRVARRDLLASAT"
     gene            complement(6873..7175)
                     /locus_tag="PLK64_09185"
     CDS             complement(6873..7175)
                     /locus_tag="PLK64_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HPV79962.1"
                     /translation="MATELVCPKCHGAMRTYERNTVTVDQCVDCRGIFLDRGELDQLL
                     DAEATYNQSAPPPAAVPPAGYPLDDNRQRGGHHGGGWDQGGGHNKRRKGFLGELFD"
     gene            7277..7678
                     /locus_tag="PLK64_09190"
     CDS             7277..7678
                     /locus_tag="PLK64_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPV79963.1"
                     /translation="MVEGAQVVGLDACAATQVDDERVAATRPLVVSAREASGIADVFG
                     LLSDPSRVRVLFALLEAGEMCVCDLAEMVEMSPSALSHALRLLRTAGVVTNRRDGRMV
                     RYRLADSHVRLLLDVTREHLGHGPAQAGRAI"
     gene            7678..>8039
                     /locus_tag="PLK64_09195"
     CDS             7678..>8039
                     /locus_tag="PLK64_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPV79964.1"
                     /translation="MGHDHGHASAATAGAKHRKPLIIAFGLTAAYMLVELVTGLVTGS
                     LALLSDAAHMGTDVLGLGMALAAITLAQREAPAQRTYGTYRLEVLAALANGLLLFGVA
                     GYVLYEAWQRFASPPDVPG"
BASE COUNT         1372 a         2660 c         2603 g         1404 t
ORIGIN      
        1 cggagaacac gtcaccaccc tcggcgtgca gagccagcca gccacctacc tcggggacct
       61 gcgccacctc accacactac tgctgcacct gggccaccaa cccggcgcgg acaggctcgc
      121 cccctgggtg cccctcctga cggcatccac cagtgaccgc ggcggccaac gcggcccacg
      181 gtggggacta cgcccgccaa ccactgccgg gctacgcgcg cagaccctgt ccaccgcgga
      241 cgcgatgctc gcggcatccg acctcgacgc cgcggtcgaa gcgttccagc cctgggtcga
      301 gctcaccccc aacaccccag acggtgtcct cggctggctc gccgaccgca ccgtgatgac
      361 ccccacactg acgcgcctgg tcatggccgc gcgagcacca cagcggcgcc tgtcccacca
      421 cctcgacacc cccagaactg agggagtgcg tatgccgatc aacctgctcg ccatccccca
      481 agtcatcccg cacgagcaat acgtcgaaca cctcaccggc gtcttcgact ccagcgaagt
      541 gaccgtccgc ctcttcgcgt cgctgtcgtt ggcacggctg cacccggacg tgacgtcctg
      601 ggccgcggct gccgaagccc tggccatgcc cggtccgatc ggagtgcggt gcgcccgggc
      661 ctgctcggcg accatgctcg tcgccgcgga cgaatgggag gctcggaccc ggcgggccat
      721 cgaagaggcc ccccgcgaga actaccgggc ccttgaggcc aagatccggc acagcctcgg
      781 gatgagccgc tggtacgacg aatgggcccg ccgctaccgg cccggcaccc ggcacgtctc
      841 ctacgacctc ggcctgtcct ggctttgggt ccacgtcgcc cacggtcacc tcgatctctc
      901 accggcctgg cgaggaaggg aaccgtcctc caaggaccgt gccccctacc gaaagttcga
      961 aagctccctc aaccaagagc agcaggtcga gctcgccaac gctgtctaca agcgagcgtg
     1021 acgacaaggg ttggcctgtc ggcgtcggca ccagtcgcct cagcgggccg acgaagtgcc
     1081 agtgtcgacc ttcatgctgg tgactctcga ccagcgatcg tgtctggcat cgacgccaat
     1141 aacgactgct cccacggtga accacaccgc cacgacggag atccctaccc agcctgaccc
     1201 cgggcctagg atggcttcgt ggctggccga cacttgaagg aaatctttcg cgcgttccac
     1261 gcgcgagacg agttggcctt ccgtcgggcg gcaatggaga tcatcgagga ggagcaagcc
     1321 aagcaccaca acgcgcttgc ccgcgatctt cagaagcttc tcgttgcagg cggtggttcg
     1381 ctcgaccctg gctcgacgat cgtcgtcccc gcgcctccca aggaccgaga aggcgagtgg
     1441 gacttgggtg aggtccgaga gccggaccgg ctcttggacg agctcgtgtt gagcgaggcc
     1501 gtgataccgg tcctggagag cctcatcgaa gaggtgcgcc actggccagc cctggacgca
     1561 gccggcctgc ctagacgcca gcgtctcctc cttcaaggcc cgcccggatg cggcaagacg
     1621 acagcggctg aggccatcgc tgctgagttg gggcggccgc tcctaatagt caggctggat
     1681 gctgtggtct cctcgtactt gggcgagact gcgagcaacc tccgccgaat cttggagtac
     1741 gccgaccagg cgccgttcgt ggtcctgttc gatgagttcg atgcgttggg ccgttcacgc
     1801 gacgatgcat cagagcacgg tgagatgaag cgcgtcgtca cggcatttct ccaaatgact
     1861 gaccgctacc gggggccgag cttgctgctg gctgccacaa accacccagc cttgctcgac
     1921 gaggccttgt ggcgacgctt cgacgaggta ctcacatttg gtctgccgac cgtgcaccaa
     1981 ctgcgacagg tcctcaggtt gcggctgaag ggcgtcgcgc actcgggact tgagatcccc
     2041 aggtatgcca gtgcccttaa gggtcgaccg cacgccgccg ccgagaaact gattatcgac
     2101 gcccggcgga atgcgctcct gcgggactca gccgtcgtga caggccagga cgtagatcgc
     2161 gtcctgccag gcataaccgc tcggccatgg tgacgggatg cccgaacacc ttctactgcc
     2221 gaaccctcgt ccgctgcagt cgcgacgttc aagccctgcg ggtggcggct cgccgaaccg
     2281 accgcatggc cagcacggtg ggcacctgcg ccagcagcta cgaaccgcac gtgccccgcg
     2341 ccagctgaac gctggcatcg accccgatct cgtattcaag atcaaggccg gttcccgacc
     2401 tgaggattct gcgttcgagg gacgcggact ccaggttctc ggcgagtcga tcgactacac
     2461 gtactttgtg ctcgcgagtg atcagggcgc ctccttggag caggcgatcg agcagtatgt
     2521 tcgaaccggg gaactgcgga ctttcttcaa ccagatcgac gacatcgagc catacggtcc
     2581 aggcgaccgc accggccctg gcattgccga cctagacggc agcaccagcg gacagcacac
     2641 agttgacgtc atcatctggc ccagcggcac gtacgttgaa gctcaacggc gagccagcat
     2701 cgtcgagcag atcgtgggtg agaatggcgg ccagaccgtg cttcgatctg tctcgcctcg
     2761 gcgtagttat ctgagggtaa gcgtctcttc cgtcggactc gacgacctcc tgaatacctc
     2821 tgtggtcgag ttggtgcgta cgccaccggt cccgttcttg gacttccgag attggaggaa
     2881 cgtcgacatc tcgacaatta gtcggtcgga ggtagccagc gaggtggttg gtgtccttga
     2941 cgactcgccg gagtcagcac atcccctgct ccgcggactg gttatgtcag acgagtctct
     3001 ggcgccgcct acctaccagt ggcagcagcg cggaacgcac ggcacggagg tagtcggacg
     3061 cgtcctcttc cccgcactcc atgaagagct ccgggatgcc gtgccgattt cggccatcgg
     3121 gactgtccgg gtcgttcgca tccttgagcc cgacccctcg cgtccagacg gtgcaccccg
     3181 gttcgcgact tacgcacctc cgcacctttt ggtcgaggaa gccattcgtc accttcacgg
     3241 cactcatggg gtcaggatct tcaacctgtc cattggctac tcagagccgt tcaacgacct
     3301 ccacctcggc cccctcacgg agacgatcga cgacctggtt cgtgagttag gcctcgtcgt
     3361 ggtgctacct accgggaacg cgcccgccaa cctctcggcc cgaactccaa gcgggcacca
     3421 catcatcgcc gacaagcccg agttcttctt cgcccccgag caccgtttgt cagagcccgg
     3481 tccggccgcc ttggccgtga ccgtaggagc gcttgctctg agtggtgcac ccgccgaact
     3541 tcctggccgc gtcggctggc aggcggctgc cgacgccgac gaggctgcac cgtttagcag
     3601 gactgggcct ggcctcggga ccaattcgcg acgtgccaac aagccggacg ttacgcatta
     3661 cggcggcaac ctcgtggtgt ccgacaccgg aaacgtcgtg cataacgatc ttggtgcctc
     3721 gcttatcagc acatctacac ggggttccgg tggccaagtc ttcgcgacgg tgaacgggac
     3781 cagttatgcc gccccagcgg tggccagagt ggcggccgat atcgcctacg cctaccccga
     3841 cgcctcagga aacctcgtca gggccctctt ggcggcgagc tcagcaccga cctctcccgc
     3901 ggccactctg gccgaactgc atcgccgaaa ccgcgtctat ggaatgggcc tcccggacgt
     3961 cgacagggcg acgtcaagcg atgccaggcg agtgacgatg acgtacgacg gcgccatgcc
     4021 agtcgatacc gtccagatcc accccctgcc gatcccggag gccttccgtc ttggtagccg
     4081 acgcgatcgc gtgatcagca tcgccctcgc gttcgaccca cctgttcggc gccagcggcg
     4141 cgagtacctc gcagggacca tgaagttcaa cgtgtatcgg gacatcagtc ctgacgagct
     4201 tgccgagatt ctgcagcggc aagaccctga cgatccggcc gatctggtca acgatcgccg
     4261 aaggctaaac ctccagccgg gcgtgaacac tttcacgcac agcacgctgc aacttcgtac
     4321 ttgggtcggg cgtaactcgt tcgtcaatga tgatgagacc ttcctcatcg ccgtcactca
     4381 caaagcgcag acttgggccc gcagcgaccc ggcctatgag caccagggct atgccctcgc
     4441 ggcaacgctc gaagaccgtg aactggcgac cgctgatttg caccagatct tgcgccagca
     4501 actacggctg ccgacccgcg tgcgcctccg cgcgtaaatc cggtgctcgc ctgagaggcc
     4561 cgccagatac ctgcgaactc cactcgcgct actcggcccc tttcctatcg ctagccctga
     4621 tcgcgcaggg ttccgaggcc cagttgtgcc atcgtcgccg catctgggca acatgctcat
     4681 cactcatgcc cacgagaacg gcgatcccac atcagagcca cgggatcatg catcggtccc
     4741 gcgccaaacg aggcactcgt cgatacgcgc gacccaccct cgattgggat gagtcttcgt
     4801 cctcctcgag agtgtcacaa tcgcctatgc catccctgcc tcctggcgcc ctccgcgccg
     4861 tgccttctgc tctgttcggc gtcggtgctg cggccggcgc ggtgggctca ggtctgggag
     4921 ccatgggtgc gcgtgacctc gttgctgcac atgagcggca ggtcgcggcg agggaacagt
     4981 atgaggcccg cgcggccgcg tcagaggatc tgcgcggggt cgccaaccag cgcctaatcg
     5041 agttctcggc tttgcagcga gcagcgttca cgggagtcgt gcagcgcatg gttgcctggc
     5101 ttcgctctca cgccaagcaa gtgcgggaga gcgacaggct cctcgtcgac ggtctcgagg
     5161 tctcgctctc gttgatgccg tccactgtgg gccgtgaaga ggccgtcgcc ggctggttga
     5221 cgggagcgtc gaacacggca ctcgctgggc taggtactgc cggtgccgtc accacggccg
     5281 tggagaagta cggagtggct ggcaccggtg caagggtctc cgatctttcc ggcgccgcaa
     5341 agcagaaggc tgcggcggcg tttcgtggtg gcggaccgct caagaacggc ggtggtggga
     5401 tcgccttggg caatctggca accaagaccg cagtcggtgg gatcagcttg cttgcggccg
     5461 gctccacctg caagatcgaa ggcgccaagg cactgacccg tgccgaggag tttgcgaccc
     5521 acattcaggt tgcgtgcgct gaatttgacg tcaaggacgc gaagctgcgc gcggttgttc
     5581 agcgcgtcga ggagctcacg gacgtgctga cgcgactcga aggacaggcc acagtcgcca
     5641 tggacaggct agaagccctc acattcgacc cgatcgagca catctctgcc ctcagcgaga
     5701 caatcgagtt ggtcaggggc gtgcagggcg tcgcgcaagc tcgtctgatc aacgagcgag
     5761 gggacctgtc gaagcaatct gagatcctga gggtccggta ccgagagatg aacggggaca
     5821 gcagcgatgg ctgacgctaa gccaatccag ggcaaggtca tggacatcaa gaccgtcgtc
     5881 gccgctcccg aggcgttcga tgcgctcacg cgcctagtcg acaccgcggg gaagtacgtc
     5941 gcgatccacg cggaggagtc gcgcaagcgg gaggcgctcc gcacctatcg cgataccgag
     6001 atcgccacca taaaagccgc ggaggtgaca ctgcagcgct acttcgacga ggcgttccgt
     6061 gaacgtcgtg aagtcttcca gagcctcttc cgggcgatgg atcatgcctt ggagaccgga
     6121 gacactcaag ggctaagcac agtcgtgagc gggatcgtcg atgtagcgaa ggactcgccg
     6181 ctagccgctg caggggatct ggctaagttg cgggccgcct tcgacgaccc caatcaagtc
     6241 tggtcgttct agggagtcgt ccgaggtgca ctcaagtttg cagtcggctc gcgtggatcg
     6301 tctttcgtgc aatctccggc gctcgtagtc gtgcagtttt gagtgcatct cgacaattac
     6361 aggaattagc cgacgttatg cacctcgggc gcgtcacgag ttcaggttat gggtcgaaca
     6421 gttgcaaggg gcgggcggca cggtctcgtc gacgatgacg cggccgcagt tgggaagtct
     6481 tgcccgaagt ctgtggcgag cgtggttgat gtgcgagccg cgggttggga cgttcggtca
     6541 cgtcgccgac gcgagcaggt cgcggcgggc aactcgctgg gtcagggtca gacgcagcag
     6601 gctcgcggtg acggcgacca tactcggcat ccacggggtg gtttcgggtt gctcgagcgc
     6661 ccagtgggtg gcggttgcca ggacgaggat gtcgcagatg gctgccgccg cgcaggccgc
     6721 gcccacggct cgacaggggg ggcgagcgcg gaagggaaga ccctccctgc caatccctat
     6781 cttctccgtg agtcgatgac catgccgaca ccgaccatca tcggaggatc cgcacgatgg
     6841 ctgggtgatc gtcggcgtgg tgtcggtcca actcagtcga agagctcgcc gaggaagcct
     6901 ttacggcgtt tgttgtgccc gccgccttgg tcccagccgc cgccgtggtg accgccgcgc
     6961 tggcggttgt catcgagggg gtagccagcc ggcggcacgg cggcgggcgg tggcgcgctc
     7021 tggttgtagg tggcctcggc gtcgaggagt tggtcgagct cgccacggtc gaggaagatg
     7081 ccccggcagt cgacgcactg gtcgacggtg acggtgttgc gttcgtaggt gcgcatcgcg
     7141 ccgtggcatt tggggcagac cagttcggtg gccatcggga tcgctctcct tgtgtcctgg
     7201 gccggcgtgg atcgacgacc tcggctgggt gaactactgt atgtgtgaca acacgatcat
     7261 atgttcagga gttccgatgg ttgagggtgc ccaggtagtc gggctggatg cgtgtgcggc
     7321 gacgcaggtc gacgacgagc gggtcgccgc gacgcggccg ctggtggtct cggcgcggga
     7381 ggcgtcgggg atcgcggacg tgttcgggct gctctcggac ccgagccggg tccgggtcct
     7441 gttcgccctg ttggaggcgg gcgagatgtg cgtctgcgac ctcgcggaaa tggtggagat
     7501 gtctccatcg gcgctctcgc acgcgctgcg gctgctccgc acggctgggg tggtgacgaa
     7561 ccggcgcgac ggccggatgg tgcgctaccg gctggcggac agtcacgtgc ggctgctgct
     7621 cgacgtgacc cgggaacacc tcggtcacgg gcccgcccag gctggccggg cgatctgatg
     7681 gggcacgacc acgggcatgc ctcggcagcg accgccggcg ccaagcaccg taaacccctg
     7741 attatcgcgt tcgggctgac cgccgcgtac atgctggtcg agctggtcac cggtctggtc
     7801 acgggctcgc tcgcactgtt gtcggatgcc gcgcacatgg gcaccgacgt cctcggcctg
     7861 gggatggcgc tggccgcgat caccctggcc cagcgtgagg cgccggcgca gcggacctac
     7921 ggcacctacc ggctcgaggt cctggccgcg ctggccaacg ggctgctcct gttcggggtc
     7981 gccgggtacg tcctgtacga ggcatggcag cggttcgcgt cgccccctga cgtgcctgg
//
LOCUS       DAOMZY010000142         8020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_8487_length_8020_cov_5.905336, whole genome shotgun sequence.
ACCESSION   DAOMZY010000142 DAOMZY010000000
VERSION     DAOMZY010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 8020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8020
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8487_length_8020_cov_5.905336"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLK64_09200"
     CDS             complement(<1..255)
                     /locus_tag="PLK64_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HPV79965.1"
                     /translation="MTSSLDPNVPAIVLGCGPVGQTAALCLAAWGVPVVMLDARPERD
                     PIGSKALCQQRDVLDVWDHVGAGRQIAAEGVTWTRAVTLYQ"
     gene            complement(413..1339)
                     /locus_tag="PLK64_09205"
     CDS             complement(413..1339)
                     /locus_tag="PLK64_09205"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HPV79966.1"
                     /translation="MAKATGVPAYTLQQLAAFVAVADTGTIAAAAVRLQLSPSAVRAS
                     LGDLERALGAQLCVRHRAKGVELTSGGKAVLAKARVVLHEANDLELDVRGEPGQVTGV
                     LHVGCYTTLGPTVLPPLLDAFRRRYPAARIELREDTLDRLRTAVDARDVDVTIAYDID
                     LPRHWSTAAWLTSAPRVHVAAGHRLAEQDGVRLLDLAEDPMILLDAPPSREHVLDMCR
                     GAGFEPRVALRTSNFETARALVGRGLGWALLVQRTATDVSYEGRQVIAKDVLEPLLPS
                     VPIVVAWPRDARLSRAAQAFVQLVARHAAQAG"
     gene            1382..2242
                     /locus_tag="PLK64_09210"
     CDS             1382..2242
                     /locus_tag="PLK64_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009357711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPV79967.1"
                     /translation="MRIDLHAHTTCSDGTQSPTELVAAARAAALDVVAITDHDTLTGW
                     AEAGAAAIEHGIALVPGVEISCERGGRSVHLLGYLVDPAYDPLAAELAKARDSRVTRL
                     ERMVALLAADGIPLTYAAVLAQVPPGATPGRPHIADALVAAGVVGSRDEAFERWLHDD
                     SPYYVHHYAPDPLRAVELVRAAGGVAVLAHPFAATRGAVILADEVISHLADAGLGGIE
                     ADHPDHDEAARARARALATRLGLFVTGSSDYHGSGKTTRLGDNLTDPVVLTALERAGS
                     GSAIIRPASH"
     gene            2269..3255
                     /locus_tag="PLK64_09215"
     CDS             2269..3255
                     /locus_tag="PLK64_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPV79968.1"
                     /translation="MSDAQPRAGQATQGEFAGMPTPLYAASPSRLLAWLDCPRRYRFQ
                     YLDRPRPAVRPPRAHTSIGVAAHNALRDWWDQSDRTPEAGARLVRESWVDLGFRDAAQ
                     SATWRERTQGEVTAYLSGVDPRRTPVGIERTAAFKTDALAFFGRIDRLDDRDGELVVV
                     DYKTGRRPLSEEDARTSLPLALYAAAVWRMFRRRCVRVELHHVPTGKVAAYEHSDESL
                     VRKVREAESIAADLRRVDAVYAVDGTDAACFPPRRSPLCQWCDYRAHCREGQQAGPEK
                     SSWAALEESTDDRVTDEAHFGGTGEVDLVVGVREPTGPASDGPQRPDGPIGG"
     gene            3275..4033
                     /locus_tag="PLK64_09220"
     CDS             3275..4033
                     /locus_tag="PLK64_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HPV79969.1"
                     /translation="MLAVANQKGGVAKTTTVASLGAAFAEMGKRVLLIDLDAQACLTF
                     SLGVDPDTVAVSINEVLIGQAGIREAIVECADGVHLVPSVIDLAGAEAQLLGRPGREF
                     VLRTILEDVAEDYDIVLLDCSPSLGVLTLAALTAAQGLLIPMPCEMLSHRGVGQLLDT
                     VRDVTRLLNPKLEVIGVLPTLFDGRSAHSQAVLADVGQRYGLPVIEPPIPKTIRFAEA
                     PAVGRSILTTSKSSKGARAYRAAAKSLAGRIWGA"
     gene            complement(4048..4449)
                     /locus_tag="PLK64_09225"
     CDS             complement(4048..4449)
                     /locus_tag="PLK64_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018774517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79970.1"
                     /translation="MTVTQALLGGAILLAAVLAIFGGSPLTGWVFAHVERRSGATPGQ
                     TVAGISAAAVVLRGGQTIGLLERTAVFLGILAGWPEALVGVIALKGLGRFAELRGTTD
                     GAAERFIIGSFTSLLWAAVLGGLARLALEHY"
     gene            complement(4446..5081)
                     /locus_tag="PLK64_09230"
     CDS             complement(4446..5081)
                     /locus_tag="PLK64_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HPV79971.1"
                     /translation="MFVVTADQVDSRREADRVPEALTLLAGVRPRAANPFERTAGDEI
                     QGVTDDPADLLDIVTSLIRADRWRLGIGIGPVDLPLPASPRAGRGPAFIAARAAISAA
                     HPATGQLAVRLGATPELDPHLRAVRERHTRYAESALLLLARLLRGRTAEGWEVTDLLA
                     DGLTQKEAAARLGVSPSAVSQRVRRSGWSEQVGAVELVQHLLSVADGRTDP"
     gene            complement(5146..5922)
                     /gene="aqpZ"
                     /locus_tag="PLK64_09235"
     CDS             complement(5146..5922)
                     /gene="aqpZ"
                     /locus_tag="PLK64_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HPV79972.1"
                     /translation="MQPALSKRLGAELLGTFWLVFGGCGSAIFAAKFYAPAGVADAQG
                     TEVGIGHLGVSLAFGLTVLTMAYAVGHVSGGHFNPAVTIGLWAARRFDNVRDLFAYIG
                     AQVVGGLLAGACLWGLAKSAKGSGSMVGNMAANGYGDNSPGGYSMWAVLLAEVLLTAF
                     FLFVILGVTTKHAPAGFGPLAIGLTLTLIHLISIPISNTSVNPARSTAVAFFNGAGAP
                     GQLWLFWVAPIVGALIAGWSYEAITGIDASAEDVSGSLAK"
     gene            6128..>8020
                     /locus_tag="PLK64_09240"
     CDS             6128..>8020
                     /locus_tag="PLK64_09240"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HPV79973.1"
                     /translation="MTSEIVWTQIDEAPALATYSFLPIVQAFTRGTGVDVVTSDISLS
                     GRILSAFPERLAPEQQVPDNLAALGALTLRPEANIIKLPNISASVPQLKAAIKELQDK
                     GYAVPDYPEAPATDEEKAVHAAYAKVLGSAVNPVLREGNSDRRAPLSVKNFAKKHPHR
                     LGAWDPATKARVETMTAGDFYGNEQSVVVDGGGDLRIEHVGADGSVTVLKDKVSTLPG
                     EIVDGTFMSVAALRDFYAAQIDAAKADGLLLSLHLKATMMKVSDPVMFGHAVSVFYAD
                     VFAKYADTLAALGVDPNMGMGDLEVKIAGLPATERAAIAADIAAVYAARPAIAMVNSD
                     KGITNLHVSSDTIVDASMPVVIRDSGQMWNWDGKLQATLAMVPDRSYGLMYAAVFEDC
                     RVNGALDPATMGSVPNVGLMAQKAEEYGSHPTTFIVPAAGSVRVLGPDGALLMSHEVG
                     AGDIWRMSRVRDIPIQDWVKLAVTRARITGAPACFYLDSARAHDRNVIAKVERYLGDH
                     DLTGVEYRILAPVEAIQWCLARIREGKDTISVTGNILRDYLTDLFPILELGTSAKMLS
                     VVPLLAGGGLFETGAGGSAPKHVQQFVAEDYLRWDSLGEFSALGASLEHLAQTQGNAK
                     AQVLAVA"
BASE COUNT         1218 a         2828 c         2796 g         1178 t
ORIGIN      
        1 ctggtagagc gtgacggccc gggtccaggt gacgccctcg gcggcgatct gacggccggc
       61 accgacgtgg tcccacacgt cgagcacgtc gcgctgctga cagagggcct tggatccgat
      121 cggatcgcgc tccgggcgag cgtcgagcat cacgacgggc accccccagg cggccaggca
      181 caacgcggcg gtctggccga cggggccgca accgaggacg atcgcgggga cgttcgggtc
      241 aagggaactg gtcatggtgt cctccagaag tcaccgaggc tcggctggca ctcatggtga
      301 ggcgttcaac aagcgctgaa aagttcaagt tatgtgggtt gttccgcagt tctggaacgg
      361 ttatgcccgc gcgcccggcc agccaccgac cccgcggacc cgacgacccc ccttagccgg
      421 cctgcgccgc gtgccgggcg accagttgca cgaacgcctg cgccgcccgc gacagccggg
      481 catcgcgggg ccacgcgacg acgatcggca ccgatggcag cagcggctcg aggacgtcct
      541 tggcgatcac ctgtcggcct tcgtaactca cgtccgtcgc cgtccgctgg acaagcagcg
      601 cccagcccag cccccgcccg accaacgccc gggccgtctc gaagttgctc gtccgcagcg
      661 ccacccgagg ctcgaatccg gcgccgcggc acatgtccag cacgtgctca cggctcggcg
      721 gcgcatcgag caggatcatc gggtcctcgg cgaggtcgag cagccgcact ccgtcctgct
      781 ccgccaggcg atgcccggcg gcgacgtgca cccgcggggc actggtcagc caggcggcgg
      841 tgctccaatg tcggggcagg tcgatgtcgt aggcgatcgt cacgtcgaca tcacgcgcgt
      901 ccaccgcggt gcgcagtcgg tcgagggtgt cctcgcgcaa ctcgatccgg gccgcggggt
      961 agcgccgccg gaaggcgtcg agcagcggcg gcaggacggt cggcccgagc gtcgtatagc
     1021 acccgacatg gagcaccccg gtgacttggc ccggctcgcc gcggacgtcc agctccagat
     1081 cgttggcctc gtgcaggacg acccgcgcct tggcgaggac cgcctttccg cctgaggtca
     1141 gctcgacgcc cttggcccga tggcgcacac acagttgcgc tccgagggcc cgctccaggt
     1201 cgccgagtga ggcccggacg gcagaggggg ataactgcag tcgaacggcc gccgcggcaa
     1261 tggtccccgt gtcggcgacg gcgacgaacg cggccagttg ctgcagggtg tatgccggca
     1321 cgccggtggc cttggccatg gcggcactct agcgactgct cccgccccta ggctcgcgtc
     1381 aatgcgcatc gacctgcacg cccacacgac gtgttcggac gggacccagt caccgaccga
     1441 gctcgtcgcg gctgcccgcg ccgccgccct ggacgtcgtg gccatcacgg accacgacac
     1501 actgacgggc tgggccgagg cgggagccgc cgcgatcgag cacggcatcg cgctcgtccc
     1561 cggcgtggag atctcctgcg agcgtggagg ccggtcggtc cacctcctcg gctacctcgt
     1621 cgacccggca tacgacccgc tcgcggccga actcgccaag gcgcgcgact cccgggtcac
     1681 gcggcttgaa cgcatggtcg cgctgttggc tgcggacggt atacccctga cgtatgccgc
     1741 ggtgctcgcc caggtgccgc cgggcgccac cccggggcgt ccccacatcg ccgacgccct
     1801 cgtggccgca ggggtcgtcg ggtcacggga cgaggcgttc gagcggtggc tgcacgacga
     1861 cagtccctat tacgtccacc actacgcgcc cgacccgctg cgggccgtcg agctcgtccg
     1921 ggccgccggg ggagtcgcgg tgctggctca ccccttcgcg gcgacgcggg gcgccgtcat
     1981 cctcgcggac gaggtgatct cgcacttggc tgacgctggg ctcggcggca tcgaggccga
     2041 ccaccccgac cacgacgagg cggcgcgagc ccgcgcccgc gcgctcgcaa cccggttggg
     2101 gctgttcgtc accgggtcga gtgactacca cggctccggc aagaccaccc ggctcggtga
     2161 caacctcacc gatccggtcg tcctcaccgc gctcgaacgc gccgggtccg gctcggcgat
     2221 catccgcccg gcaagccact gacaccccga ccggctagcg tggccagcgt gtcggacgcg
     2281 caaccacggg ccgggcaggc cacccagggt gagttcgccg gcatgcccac cccgctgtat
     2341 gccgccagcc cctcccgcct gctcgcctgg ctggactgcc cgcggcgcta ccgcttccaa
     2401 tacctcgatc ggccccgacc cgcggtccgt ccgccgcggg cccacacctc gatcggcgtg
     2461 gcggcccaca acgccctgcg cgactggtgg gaccagtccg accggacacc cgaggccggc
     2521 gcccggctgg ttcgcgagag ctgggtcgac ctcggcttcc gtgacgccgc ccagagcgcg
     2581 acctggcggg aacgcactca gggcgaggtc acggcatacc tctcgggggt ggacccgcgc
     2641 cgcaccccgg tggggatcga gcgcacggcc gccttcaaga ccgacgcgct ggcgttcttt
     2701 ggtcggatcg accggctcga tgaccgcgac ggcgagttgg tcgtcgtcga ctacaagacc
     2761 ggccgacgcc cgctctccga ggaggacgcg cgcacgtcgc tcccgctcgc gttgtatgcc
     2821 gcggccgtct ggcggatgtt ccgccgccgc tgcgtccggg tcgagttgca ccacgtgccg
     2881 acgggaaagg ttgcggccta cgagcactcg gacgagtccc tcgtgcgcaa ggtgcgcgaa
     2941 gccgaatcga tcgctgcaga cctgcgacgg gtcgacgccg tgtatgccgt cgacggcacc
     3001 gatgcggcgt gcttcccgcc acggcgctcg ccgttgtgtc agtggtgcga ctaccgtgcc
     3061 cattgtcggg aagggcagca ggccggtccc gagaagtcgt cctgggccgc actggaggag
     3121 agcacggatg accgcgtcac cgacgaagcg cacttcggcg gcacagggga ggtcgacctc
     3181 gtcgtcggcg tccgggagcc cacaggtcca gcgtcggacg ggccgcagcg cccggacggg
     3241 ccgatcggcg gttgaggccc gcaccgggcc gttcgtgctc gccgtcgcca accagaaggg
     3301 tggcgtggcc aagacgacga ccgtcgcgag cctgggtgcg gcgttcgcgg agatgggcaa
     3361 gcgggtgttg ctcatcgacc ttgacgcgca ggcctgcctc accttctccc tcggggtgga
     3421 ccccgacacc gtcgccgtct cgatcaacga ggtgctcatc ggtcaggcag gcatccgcga
     3481 ggccatcgtc gagtgcgccg acggcgtcca cctggtgccg tcggtcatcg acctggccgg
     3541 ggcagaggcc caactgctcg gtcgtcccgg gcgggagttc gtgttgcgca cgatcctgga
     3601 agatgtggcg gaggactacg acatcgtcct gttggactgc tcaccgagcc tgggggtgct
     3661 caccctcgcc gcgttgacgg cggcccaggg cctgctcatc cccatgccgt gcgagatgct
     3721 cagccatcga ggggtcggcc agctgctcga cacggtgcgt gacgtcaccc ggctgctcaa
     3781 ccccaaactc gaggtcatcg gggtcctgcc aacgctgttc gacgggcgct cggcgcactc
     3841 gcaggcggtg ctcgccgatg tcgggcagcg ctacggtctg ccggtcatcg agccgccgat
     3901 ccccaagacg atccggttcg ccgaggcccc ggccgtgggg cgctcgatcc tcacgacctc
     3961 gaagtcgtcc aagggcgccc gcgcctatcg ggcggccgcg aagtcccttg ccggccgaat
     4021 ttggggtgcc tgagtcacag cgcggcgtca gtagtgctca agggcgagcc gggccagccc
     4081 gcccagaacg gcagcccaga gcaggctggt gaagctgccg atgatgaacc gctcggcggc
     4141 gccgtcggtc gtgccgcgca actcggcgaa tcggcccaag cctttgaggg cgatgacgcc
     4201 cacgagggcc tcgggccagc cggcgaggat cccgaggaag acggccgtgc gctcgagcag
     4261 gccgatcgtc tgcccaccgc gcaacacgac cgcggcggcg gagatgcccg ccaccgtctg
     4321 ccccggggtt gcaccggagc ggcgctcgac atgggcgaag acccagccgg tgagcgggct
     4381 gccgccgaag atcgccagca cggcagccag caggatggcg ccgccgagca gcgcttgggt
     4441 cacggtcacg ggtcagttct accgtctgcc accgacagca ggtgctggac gagctcgacg
     4501 gcgccgacct gctcgctcca cccggagcgc cggacccgct ggctgaccgc tgacgggctg
     4561 acccccaagc gggccgccgc ctccttctgg gtcaacccgt cggccaagag gtcggtcacc
     4621 tcccagcctt cggccgtccg cccgcgcagc aggcgcgcga ggagcaagag ggctgactcg
     4681 gcatatcggg tatgccgctc gcgcaccgcc cgtaggtgag ggtccagctc cggcgtcgca
     4741 ccgaggcgga cggccagttg gccggtcgcg gggtgggctg ccgaaatcgc ggctcgggcg
     4801 gcgatgaagg cgggcccgcg gccggctcgc ggcgacgccg gcaggggcag gtcgaccggg
     4861 ccgatgccga tgccgagacg ccaacgatcg gcacggatca ggctcgtgac gatgtcgagc
     4921 aggtcggcgg gatcatcggt gactccctgg atctcgtcgc cggccgtgcg ctcgaaaggg
     4981 ttggctgctc gcgggcgtac ccccgcgagc agggtcagcg cctcagggac gcggtcggcc
     5041 tcgcggcgac tgtccacctg gtctgctgtg acgacgaaca tgtccatcct tcattccgat
     5101 aaatgaagga taacaacttc actcgctggt gtgaagcctc ttgccttact tggcgaggct
     5161 cccggacaca tcctcggcgc tggcgtcgat gccggtgatg gcctcgtagc tccagcccgc
     5221 gatgagcgca ccgacgatcg gggccaccca gaacagccac aactgcccgg gggcgccggc
     5281 tccgttgaag aaggcgaccg ccgtcgagcg ggccgggttg accgacgtgt tgctgatcgg
     5341 gatcgagatg aggtggatga gcgtcagggt gagaccgatg gccagcggcc cgaagccggc
     5401 cggcgcgtgc ttggtggtca ccccgaggat gacgaacagg aagaaggccg tgagcaggac
     5461 ctcggcgagc agcaccgccc acatcgagta gccgccgggc gagttgtcgc cgtagccgtt
     5521 cgcggccatg ttgccgacca tgcttccgga gcccttcgcg ctcttggcca atccccagag
     5581 gcacgctccg gccagcaggc cgccgacgac ctgggcgccg atgtaggcga acaggtcgcg
     5641 gacgttgtcg aagcggcggg ccgcccacag gccgatcgtc accgccgggt tgaagtgccc
     5701 tccggagacg tggcccacgg catacgccat cgtgaggacg gtgagcccga aggcgagcga
     5761 gacgccgaga tggccgatgc cgacctcggt gccctgtgcg tcggccaccc cggcgggggc
     5821 gtagaacttg gcggcgaaga tggcgctgcc acacccgccg aatacgagcc agaacgtgcc
     5881 gagtagttcg gcgccgagcc gcttgctgag tgcaggctgc atggaaggtc tcccttgtcg
     5941 ttctccgatc gggcgtttgc cgcggtcgtc ggcgtctacg ctaccggctc gccacccgcc
     6001 cggctgcgag gtggtgtgat caacccgacg agaggacggg ccgaacgcgg cataccgagg
     6061 tatcttgacg tcaagctatc ctgggtcgcg acccctgtcc ggccagcttc gaatcgacgg
     6121 agatgcgatg acctcagaaa ttgtgtggac ccagatcgac gaggcgccgg ccctggcgac
     6181 ctactccttc ctgccgatcg tccaggcgtt caccaggggg accggggtcg acgtcgtgac
     6241 gagcgacatc tcgctctccg ggcgcatcct gtcggccttc cccgagcggc tcgcgccgga
     6301 gcagcaggtg cccgacaacc tcgccgcgct gggcgcgctg acgctgcgcc ccgaggcaaa
     6361 catcatcaag ctccccaaca tctccgcctc cgtgccgcag ctcaaggccg cgatcaagga
     6421 gttgcaggac aaggggtatg ccgtccccga ctatcccgag gcgccggcga ccgacgagga
     6481 gaaggcggtg cacgcggcat acgccaaggt tcttggctcc gcggtgaacc ctgttctgcg
     6541 cgaaggaaac tccgaccgcc gcgccccctt gtcggtgaag aacttcgcca agaagcaccc
     6601 gcaccgcctc ggcgcgtggg acccggcgac caaggctcgc gtcgagacga tgactgccgg
     6661 tgacttctac ggcaacgagc agtccgtggt tgttgacggc ggtggcgacc tgcggatcga
     6721 gcacgtcggc gctgatggct cggtgaccgt cctcaaggac aaggtgtcca cgctgcccgg
     6781 cgagatcgtc gacggcacct tcatgagtgt ggcggcgctg cgggacttct atgccgcgca
     6841 gatcgatgcc gcgaaggccg acgggctgct gctgtcgctg cacctcaagg cgacgatgat
     6901 gaaggtctcc gacccggtga tgttcggtca tgcggtgtcg gtgttctatg ccgatgtctt
     6961 cgcgaagtac gccgacacgt tggccgcgct cggggtcgac ccgaacatgg gcatgggcga
     7021 cctggaggtc aagattgccg gcctgccggc gacggagcgc gcggcgatcg ccgccgacat
     7081 cgctgcggtg tatgccgcgc ggccggccat cgcgatggtc aactctgaca agggcatcac
     7141 caacctgcac gtctccagcg acacgatcgt cgacgcgtcg atgccggtcg tcatccgcga
     7201 ctccggccag atgtggaact gggacggcaa gctccaggcg accctcgcca tggtgcccga
     7261 ccgctcctac ggcctcatgt atgccgcggt gttcgaggac tgccgcgtca acggcgccct
     7321 cgacccggcg accatgggct cggtgcccaa cgtcgggctg atggcgcaga aggccgagga
     7381 gtacggctcg cacccgacga cgttcatcgt gcctgccgcc gggagcgtgc gcgtgctggg
     7441 gcctgatggc gccctgctca tgtcgcacga ggtcggtgcc ggggacatct ggcggatgtc
     7501 gcgcgtgcgg gacatcccga tccaggactg ggtcaagctc gcggtgacgc gcgctcggat
     7561 cactggcgcc ccggcgtgct tctacctcga ctccgcgcgg gcgcacgacc gcaacgtcat
     7621 cgccaaggtc gagcgctacc tgggcgacca cgacctgacg ggtgtggaat accggatcct
     7681 ggctccggtc gaggcgatcc agtggtgcct cgcgcggatc cgcgagggca aggacaccat
     7741 ctcggtgacc ggcaacatcc tgcgcgacta cctcaccgac ctcttcccga tcctcgagtt
     7801 ggggacctcg gccaagatgc tctcggtcgt gccgctgctc gccggtggtg ggctcttcga
     7861 gaccggtgcc ggtgggtcag cccccaagca cgtgcagcag ttcgtcgccg aggactacct
     7921 gcgctgggac tcactcggtg agttctcggc gttgggtgcc tcgcttgagc acctggccca
     7981 gacccagggc aacgccaagg ctcaggtgct tgcggtcgcg
//
LOCUS       DAOMZY010000143         7997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_8516_length_7997_cov_6.032863, whole genome shotgun sequence.
ACCESSION   DAOMZY010000143 DAOMZY010000000
VERSION     DAOMZY010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7997
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8516_length_7997_cov_6.032863"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3058)
                     /locus_tag="PLK64_09245"
     CDS             complement(<1..3058)
                     /locus_tag="PLK64_09245"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HPV79974.1"
                     /translation="MTAPMALADPTSPLTVTNVANPSPVASGAELTYTITIVNTGGSK
                     VSNLVLADQLNGVGTLQSPPGTPQYAVTSTQGTCTQSGQLVTCNGGTLAGGASWTVTI
                     RGVVMAANGVTINNTASITGTRSAQNFTTNASVQTLVQGGTGGGSLPDLTINKTGPTT
                     VVAGAAFDYVLTVNNVGTVATSNVAVTDTLPAGVALTSITTTSLFVCSPTNGTGVTVV
                     CTGGSVNAGQNATITLHVTAPATGPLTNTASVDPDNTIAESNELNNTSATVNTSVTGA
                     TAVPPLSIIKTDDPAVIAGAGPDPVSPGGVLVYKVRVTNVGTLRADDVVVVDGTQGLE
                     AASIVATQAVVNGTVGNGNGCFVSAPEVRCKIRTLNAGGTLTITITGTVIAPAGATLI
                     NTATVTGNVRNTGVTATATEKTTVKPAVDLTVTKSASPDPVCARSWPHANPDDLCTGG
                     LVYTFVVGNSGIQSASNVVVRDVLPAGTIYDEFANVGGADFSCSESGGVLTCTNPSIA
                     PESIESFTVTLVAPDSVGPLTNSVTVDPGNAIFEADETNNSASVTSQVSTGIDLTIFK
                     FDEPGATADPAGSIPNYPSPATGFDPIATSGTQTYTIYVDNLGPQNATDVRVVDTLPA
                     GTKFLSATSDAGFTCTHDGSATGGVVTCVGGSLLGTEAEFYPGHGGVINPANTQFATI
                     VIKAFATPFVQPVMHNEVRVDPLGAIAEINEGNNIATQNTVVETGGAGLSAFNQLTIA
                     KAQTDPVPPAAVATNGILRYTLTVGNDGTDPVSNVVVKDFLPSGSRFIAAADTDSGLG
                     TADAFFCTHDGAATGGVITCIGGDLSGTVNTIPDSGGVGDVPTSRQILITVYAPGTPG
                     AYTNVAKVDPDNLVPEGNEFDNEAQASTTVTTAGNGGANSFHQLTIGKSATPTVATSS
                     IITYTLSVANLGTDPAFGVAVRDTLPAGTTFISADDQAPGATQFTCAVSGQVITCSGA
                     TLSGTATVLPGAPATRTIVITAFAPNRPMIVTNTAYVDPDNTIPEGD"
     gene            complement(3439..3642)
                     /locus_tag="PLK64_09250"
     CDS             complement(3439..3642)
                     /locus_tag="PLK64_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPV79975.1"
                     /translation="MAQGVVKWFNAEKGFGFIAQDGGGPDVFVHYSAIQTQGYRSLDE
                     AQRVEFEVTQGPKGPQADAVRPL"
     gene            complement(3961..4902)
                     /gene="folP"
                     /locus_tag="PLK64_09255"
     CDS             complement(3961..4902)
                     /gene="folP"
                     /locus_tag="PLK64_09255"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPV79976.1"
                     /translation="MSTEPPGNTPAPLVLRGQTFDAARPAVMAIVNRTTDSFYVGNRH
                     ADLDSALAALDSAVALGAEIVDIGGVRAGQEGEAVTPGMEIDRVMPFLERARAAYPDL
                     ILSLDTWRSEVARAAAHAGVDLVNDTWAGYDPDLLATAVEIGAGYVISHTGGYPPRTD
                     PVDVTYGPGELDVVDDVVSTLRSGAVRAVAAGMPRERVLVDPTLDFGKTTTHSLRVLR
                     HTEAVTALGFPVLQALSRKDFVGETLDLPADERLEGSLAATAVAAWLGATVFRAHDVG
                     ATRRVVDMVASIRGDRPPLVSVRGIGPHSRPADGATP"
     gene            complement(5153..6046)
                     /locus_tag="PLK64_09260"
     CDS             complement(5153..6046)
                     /locus_tag="PLK64_09260"
                     /inference="COORDINATES: protein motif:HMM:NF017672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV79977.1"
                     /translation="MPPAPGSPAPGPSAPDRRRTQSTPAPAGVLGVLVGGAALLVTLA
                     ALVAWLSPATLGERPATCLSTSGARCFCEAVGTGLLRQPANSLSSLGFCVAAATTAAR
                     GRRLGRHTPERVLAPVVALTLVALGACSLAYHAQLTFAGQVLDVQGMYLLGTLLVVGA
                     LWRRGTLDATRAAAWAAALLLTLLLAQAILPDARRWLFALILLPGIVLEWRLAPRSRP
                     LAIAVLALAIAYAAWLADDRGLWCEPGSWLQGHALWHLLTALAGGLLVAHYRRTASGH
                     QNAPGANAGGETELPRVVGQT"
     gene            complement(6080..6928)
                     /locus_tag="PLK64_09265"
     CDS             complement(6080..6928)
                     /locus_tag="PLK64_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPV79978.1"
                     /translation="MHNVILNNGVEMPILGFGVFQIPDDETQAAVEAALAAGYRHLDT
                     AASYGNEEAVGRAIVASGIARSDIFVTTKLWIQSNPTQTKARAALEKSLERLGLDAVD
                     LYLIHQPFGDYYGSWRAMQTALGEGLARAIGVSNFQPDRLVDLIEHNEIAPAVNQIET
                     HPFFQRQIDQDVMRERGVQIESWGPFAEGRNGLFTNPELSEIAAARGKSVAQVVLRWL
                     IQREVVVIPKSVRPERLAENLDVFGFELTADDMSRIATLDTGASVFFDHRDPAMVSWL
                     NGRREA"
     gene            7041..7754
                     /locus_tag="PLK64_09270"
     CDS             7041..7754
                     /locus_tag="PLK64_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV79979.1"
                     /translation="MSERATYHHGDLRQALIAAAVDLISAKGVAGLSVAEAARKAGVS
                     GAAPYRHFASRTALLSAAATALATDLLEHLQVTAAGGPARGVPTVARGVPLAPALAVP
                     HAADPTDVAAAIDSLAQLTRARIDFALGRGVGLELVYAEELRTFDDPQRRDVTRRLFE
                     LYLWPAMTVTGDAVAAGRLLRQAAALAQGYLDIARSNNAVAGTTEAAELADEAAAAVR
                     VLAHAAQDRRGTVASDPPT"
BASE COUNT         1238 a         2534 c         2916 g         1309 t
ORIGIN      
        1 cgtcaccctc ggggatggtg ttgtccgggt cgacgtatgc cgtgttcgtc acgatcatcg
       61 gccggttggg cgcgaaggcc gtgatgacga tggtgcgggt cgccggagca cctggcagca
      121 ccgtggcggt gccgctgagg gttgccccgg agcaggtgat cacctgaccg ctcaccgcac
      181 aggtgaactg ggtggcgccc ggggcctggt cgtccgccga gatgaaggtc gtcccggccg
      241 gcagggtgtc gcggacggcg accccgaagg ccgggtcggt gccgaggtta gccacggaca
      301 gcgtgtaggt gatgatgctg ctcgtcgcaa cggtcggcgt ggccgacttg ccgatggtca
      361 gttggtggaa ggagttggcg ccaccgttgc cggccgtcgt gaccgtcgtg ctcgcctggg
      421 cctcgttgtc gaactcgttg ccctcgggga cgaggttgtc cgggtcgacc ttggccacgt
      481 tggtgtaggc gccgggggtg ccgggcgcat acacggtgat gaggatctga cggctggtcg
      541 gcacgtcccc gacgcctccg gagtcgggaa tcgtgttgac cgtcccggag aggtcaccgc
      601 cgatgcaggt gatgacgccg cccgttgctg ccccgtcgtg ggtgcagaag aaagcgtccg
      661 ccgtgccgag tcctgagtcg gtgtcggcag ccgctatgaa ccgggagccc gacggcagga
      721 agtccttgac gacgacgttg ctcaccggat cggtgccgtc gttgccgacg gtcagggtgt
      781 agcgcaggat gccgttcgtg gcgaccgcgg cgggcgggac cgggtcggtc tgcgccttgg
      841 cgatggtcaa ctggttgaag gcgctcaggc cggcgccacc ggtctccacg accgtgttct
      901 gggtggcgat gttgttgccc tcgttgatct cggcgatggc gccgagcggg tccacccgca
      961 cctcgttgtg catgacgggc tggacgaacg gggtcgcgaa cgccttgatg acgatcgtcg
     1021 cgaactgtgt gttggccggg ttgatgacgc cgccgtggcc ggggtagaac tcggcttcgg
     1081 tgcccagcag gctgccgccg acacaggtga cgacgccgcc ggtggcggac ccgtcgtgcg
     1141 tgcaggtgaa gccggcgtca ctggtggccg agaggaactt cgtccccgcg ggcaacgtgt
     1201 caacgactcg gacgtcggtc gcgttctgcg ggccgaggtt gtcgacgtag atcgtgtagg
     1261 tctgggtgcc gctggtcgca atcgggtcga agccggtcgc cgggctcggg tagttaggga
     1321 tgctgccggc ggggtcggct gtcgcgccgg gctcgtcgaa cttgaagatc gtcaggtcga
     1381 tgccggtgga gacctggctg gtcaccgagg cactgttgtt ggtctcgtcg gcctcgaaga
     1441 tcgcgttgcc cgggtcgacg gtgaccgagt tggtcagcgg gccaaccgag tccggcgcca
     1501 cgagggtgac ggtgaaggac tcgatggact caggggcgat gctggggttg gtgcaggtga
     1561 ggacgccacc gctctctgag cacgagaagt cggctccacc cacgttggcg aactcgtcgt
     1621 agatcgtccc cgcagggagc acgtcccgca cgacgacgtt ggacgcagac tgtatgccgc
     1681 tgttcccgac gacgaaggtg tagaccagcc cgccggtgca caggtcgtcg gggttggcgt
     1741 gcggccagga gcgggcgcag accgggtcgg gtgacgcgct cttggtcacg gtgaggtcga
     1801 cggcgggctt gaccgtcgtc ttctccgtgg cggtcgcggt gacgccggtg ttgcggacgt
     1861 tgccggtcac agtcgccgtg ttgatgaggg tcgcaccggc gggagcgatg accgtgccgg
     1921 tgatggtgat ggtgagcgtg ccgcccgcat tgagggtacg gatcttgcag cgcacctccg
     1981 gcgccgacac gaagcagccg ttgccattgc ccacggtgcc gttgacgacg gcctgcgtgg
     2041 cgacgatgct cgcggcctcc aggccttgag tgccatcaac gacgacgacg tcgtcggcgc
     2101 gcagcgtgcc gacgttggtg actcgcacct tgtagacgag cacgccaccg ggggacaccg
     2161 gatcgggtcc ggcaccggcg atgacggctg ggtcgtcggt cttgatgatc gacagcggcg
     2221 ggaccgcggt ggcgccggtg acgctcgtgt tgacggtggc cgaggtgttg ttgagttcgt
     2281 tgctctcggc gatcgtgttg tcagggtcga ccgatgccgt gttcgtcagc ggcccggtgg
     2341 ccggggcggt gacgtgcaag gtgatcgtcg cgttctgacc ggcgttgacc gaaccgcccg
     2401 tacacacgac ggtgacgccg gtgccgttcg tcggggagca gacgaacagg ctcgtcgtcg
     2461 tgatcgaggt gagagccacg ccagcgggca gggtgtcggt gacggcgacg ttggacgtcg
     2521 cgacggtccc gacgttgttg acggtcagga catagtcgaa ggcggccccc gcgacgacgg
     2581 tggtcggacc ggtcttgttg atcgtcagat cgggcaggct acctccgccg gtgccgccct
     2641 ggacgagcgt ctgaacgctc gcgttggtcg tgaagttctg cgccgaccgg gtcccggtga
     2701 tcgacgccgt gttgttgatc gtcaccccgt tggccgccat gaccaccccg cggatagtga
     2761 cggtccagct cgcgccgccc gccaacgtcc cgccgttgca ggtgaccaac tggccgcttt
     2821 gggtgcaggt gccctgggtg gaggtcacgg catactgcgg cgtccccggt ggggactgca
     2881 gggtgccgac cccgttgagc tgatcagcga ggacgaggtt ggagaccttg gagcccccgg
     2941 tattgacgat ggtgatcgtg taggtgagct cggcgcctga tgcgaccggg ctggggttgg
     3001 cgacgttcgt caccgtgagc ggactcgttg gatcagccag agccatcgga gcggtcacga
     3061 ggccgagggt gaggagtcca aaggtcgcca ggacgcttac cgtccgacga atcgtgctgc
     3121 gtagggaaat ccgggcaggg gacggcaagg caacctgtga catgttcaac cggtccttaa
     3181 ggggagagtc aggacccccg agtgtgaaca tcgccaacca agtcctcacg acctggtatg
     3241 tcaatggccc tgcgagtagt cactggacta gtcatcctcc agagcaggct cgcctggtac
     3301 ctgggaggag tgtgctcggg ttcgccagat acctgcagga accggtgatc ttccagcctg
     3361 agaacacacg tgccccccgg accgacgtcc ggggggcccg tgtcgctgac cggggtcagc
     3421 tgcgaggaac tcgcgtgatt acagcgggcg gacggcgtcc gcctgcgggc ccttggggcc
     3481 ctgggtcact tcgaactcga cgcgctgtgc ctcgtcgagc gagcggtaac cctgagtctg
     3541 gattgctgag tagtgaacga aaacgtcggg gccgccgccg tcctgggcga tgaaaccaaa
     3601 gcccttctca gcgttgaacc acttgacgac tccctgtgcc atggggacaa ctctctctat
     3661 aactggacac tggagcccgc acctcacgaa ctcccgtgct gctgccacaa cccaccgctc
     3721 gcgagcctgc gcccgagtgc ttgaacacga cgtccgctgc ccaaaggcga tgggcgtcgt
     3781 gtccgtcttg cgaggaactg caactgcaac cggcgccgac gttagcacgc agtgatcggc
     3841 actacacctg ctcagaccca ccattttttt gggctaccga cgggtagtct cacgggtcga
     3901 ccagcgcccc tcccccgacc tacgaaaagg tctgcgtcag aacctttttg ggtgcatcgc
     3961 tcagggcgtt gcgccgtccg ccggacggct gtgcgggccg ataccgcgca cgctcacgag
     4021 gggcggccga tcgcctcgga tcgaggccac catgtcgacc acccgacggg tcgcgccgac
     4081 gtcgtgggcg cggaataccg tcgccccgag ccaggccgcg acggccgttg cggccaggct
     4141 gccttcaagg cgttcgtcgg ccggcaggtc gagggtctcg ccgacgaagt ccttgcggga
     4201 caacgcctgc aacaccggga agcccagggc cgtcaccgcc tcggtatgcc gcagcaccct
     4261 cagcgagtga gtcgtcgtct tgccgaagtc caaggtgggg tcgacgagga cccgctcgcg
     4321 cggcataccc gcagcgacag cccgcacagc gcccgaccgc agcgtcgaca cgacatcgtc
     4381 gaccacgtcg agctcgccgg gaccgtaggt gacgtccacc ggatcggtgc gcggcgggta
     4441 gccgcccgtg tgcgagatga cgtacccggc cccgatctcc acggccgtcg ccaacaggtc
     4501 cgggtcgtag cccgcccagg tgtcgttgac caggtcgacc cccgcgtggg ccgccgcacg
     4561 ggccacctcg gaccgccagg tgtcgaggga gaggatgaga tcggggtatg ccgcgcgcgc
     4621 ccgttcgagg aacggcatca cccgatcaat ctccatccca ggcgtgaccg cttcgccctc
     4681 ctgcccggcg cggaccccgc cgatgtcgac gatttcagcg cccaaggcga ccgcagagtc
     4741 gagggccgcc agagcgctat cgaggtcggc gtgccggttg cccacgtaga aggaatccgt
     4801 cgtccgattg acgatggcca tcaccgcagg acgggccgcg tcgaaggtct gaccccgcag
     4861 gacgagaggt gcgggggtgt tgccgggagg ctcggtgctc acggcgtcat cgtcgcacgc
     4921 cagggtattc cccagggtcc gcgtgtgctt cggcatgctc cgccgattgg ccagcagcat
     4981 gctcgaaatg gtatgataga caacgagtct ccgatatgga ggcgcgtaac ccattgatac
     5041 gaaacgaagt tcacacgatg cccagctgga ttttctacct tccgatgttt ggccgcacca
     5101 cctcgcacgc gggggcctac cactacgacc gtcgcaacat ggtcatggga cgtcaggtct
     5161 gacccacaac tcgcggcagt tccgtttcgc ccccggcatt ggcgccgggg gcgttttggt
     5221 gtcctgacgc ggttcggcgg tagtgggcga cgagcagccc accagccagc gcggtgagca
     5281 gatgccagag ggcgtggccc tgcagccagg agcccggctc gcaccacaga ccccgatcgt
     5341 cggccagcca cgcggcatac gcgatggcga gggcgagcac cgcgatggcg agggggcgag
     5401 agcggggggc cagccgccat tcgaggacga tcccggggag caggatgagc gcgaacagcc
     5461 agcgccgcgc gtccggcagg atcgcttgcg cgagcaatag cgtgagcagg agggccgccg
     5521 cccaggctgc cgcgcgggtg gcgtcgaggg tgccacgccg ccagagggca ccgacgacga
     5581 gcagggttcc caggaggtac atgccttgca cgtcgagcac ctgcccggcg aaggtcaact
     5641 gcgcgtgata ggccagactg cacgccccga gcgcgacgag ggtcagcgcg acaacggggg
     5701 cgaggacccg ctcaggggta tgccgcccca gtcgcctccc gcgcgcggcc gtcgtggcgg
     5761 ctgcgacgca gaagccaagg ctggacaggg aattggccgg ttggcgcagc aggcccgtgc
     5821 cgacggcctc gcagaagcag cgcgcgcccg acgtgctgag gcaggtcgcc ggtcgctcgc
     5881 cgagcgtcgc cggggaaagc cacgcaacga gcgcggccag ggtgacgagc agcgcggcgc
     5941 ctccgacgag gacgccgagg acgcccgccg gggcgggtgt gctctgcgtg cgacgacggt
     6001 caggtgccga cggtcctggc gcgggcgatc ctggcgcggg cggcataccc aggagtctgt
     6061 cgcatgaccg cgcgaggagt taggcctcgc ggcgaccgtt gagccagctg accatcgcgg
     6121 ggtcacggtg gtcaaagaag acggacgcgc cggtgtcgag cgtggcgatc cggctcatgt
     6181 cgtcggcggt gagctcgaag ccgaagacgt cgaggttctc ggccagtcgc tcggggcgca
     6241 ccgacttggg aatgacgacg acctcgcgct ggatcagcca acggagcacg acttgagcga
     6301 ccgacttgcc gcgggccgcc gcgatctcgc tcaactcggg gttggtgaag aggccgttgc
     6361 ggccctcggc gaatggtccc cacgactcaa tctgcacgcc gcgctcgcgc atgacgtcct
     6421 ggtcgatctg acgctggaag aacgggtggg tctcgatctg gttgaccgcc ggcgcgatct
     6481 cgttgtgctc aatgaggtcg acgagccggt cgggctggaa gttggagacg ccgatcgcgc
     6541 gagccagacc ctcaccaagg gcggtctgca tggcccgcca cgagccgtag tagtcaccga
     6601 acggctggtg gatgaggtag aggtcgacgg cgtcgaggcc gaggcgttcg agcgacttct
     6661 cgagggccgc gcgggctttg gtctgggtcg ggttgctctg gatccacaac ttcgtcgtga
     6721 cgaagatgtc cgatcgcgcg atgccgctgg ccacgatcgc ccgccccacg gcctcctcgt
     6781 tcccgtaaga cgccgccgtg tcgaggtgtc ggtagcccgc ggcgagcgcg gcctccaccg
     6841 ccgcctgggt ctcgtcgtcg gggatctgga agacgccgaa gccgaggatc ggcatctcga
     6901 cgccgttgtt caggatgacg ttgtgcatgg gaggcctctc gccggatctt cgggtgtggt
     6961 ggtcgcgtgc caccttgcgt gcagggtcta atgtaagaga ctcttacatt gatggtcaac
     7021 cgggctgcgc gggaggtgac gtgagcgagc gagcgaccta ccaccatggc gatcttcgtc
     7081 aggcgctcat tgccgctgcc gtcgacctga tctcggcgaa gggcgtcgcc ggcctctccg
     7141 ttgcggaggc tgcgcgcaag gcaggggtga gcggggccgc cccgtaccgg cactttgcca
     7201 gccgcaccgc gctgctgtcg gcagccgcga ccgcgttggc gacggacctg ctggagcatc
     7261 tgcaagtgac ggctgccggg ggtcctgccc gtggcgtccc cactgttgcc cgcggcgtcc
     7321 cccttgcccc tgcccttgcc gtcccccatg ctgctgatcc cacggatgtc gccgccgcga
     7381 tcgactcact ggcgcagctc acccgcgcgc ggatcgactt cgccctgggg cgtggggtcg
     7441 gccttgaact cgtctatgcg gaggaattgc gaaccttcga cgacccgcag cggcgggacg
     7501 tcacccgccg cctgttcgag ctctatctct ggcctgccat gacggtgacg ggcgacgccg
     7561 tcgctgctgg acggctgttg cgtcaggctg cggcgttggc ccagggctac ctcgacattg
     7621 cacgaagcaa caacgccgtc gccggcacga ccgaagccgc ggagttggcc gacgaggcag
     7681 cagccgcggt ccgggtccta gcccacgccg ctcaggaccg gcggggcacg gtggcgagcg
     7741 atccgccgac gtagggatcg acgaagggga tcggcgcagg gatcgaaccc gccgggcgag
     7801 caaccggcag gagggctgaa aagtctgagt gcctcaggta cgcagtgcgt gcctgaggta
     7861 cgggttcttc acgggggtcc tggcaggtcg ggaaccaggc gagcgccagc gagcggaggg
     7921 cgtggtgggt cctacggcca ccgcgagcgc cagcgagcgg agggcgtggt gggtcctacg
     7981 gccaccgcga gcgccag
//
LOCUS       DAOMZY010000144         7992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_8523_length_7992_cov_7.187098, whole genome shotgun sequence.
ACCESSION   DAOMZY010000144 DAOMZY010000000
VERSION     DAOMZY010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7992
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8523_length_7992_cov_7.187098"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..636
                     /locus_tag="PLK64_09275"
     CDS             112..636
                     /locus_tag="PLK64_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPV79980.1"
                     /translation="MSARAPGEVGDRPPDDVDRIVAAWRRELPHLDVEPLLVLSRITR
                     LARRLDLARGVAFATHHLESWEFDVLSALRRAGRPYQLSPGQLVTETLVTSGTMTNRV
                     DRLEQRGLVRRGPDPGDRRGVKVRLTRVGREAVDAALSDLLAHERELLGELTPEEQAT
                     LAGMLRRLNAPLEP"
     gene            complement(689..2524)
                     /locus_tag="PLK64_09280"
     CDS             complement(689..2524)
                     /locus_tag="PLK64_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPV79981.1"
                     /translation="MGILVSLERVTVVHGVAHVLDALTVGVLGGQRIGVVGRNGGGKS
                     TLLRVLAGRQEPDSGRVTSVGGLTVGLLAQDDRLDPAATVRHALVGDRPEHEWAGDAR
                     VREVLDGLLGGVGMLAVGGPDALVGPLSGGERRRLALAALLVADPDLLLLDEPTNHLD
                     VEGVAWLAEHLATRRTRPDTALVAVTHDRWFLDAMATLTWEVDSGAVHSYDGGYAAYV
                     LAKAERSRVAAVTAERRDNLLRKELAWLRRGAPARTTKPKFRIDAANALIENEPPPRD
                     GVELVRFATRRLGKDVVDLFDATVRYGERTLLDKVTWQLAPGERVGIVGVNGAGKSTL
                     LQAISGDVSLAGGRRKVGKTVSLAHLTQEVRELDELAAYRVIEAIEAVRHSIMLGDRE
                     VTASQLATRLGFTGQRQQTRVGDLSGGERRRLQILRLLMAEPNVLLLDEPTNDLDIET
                     LTSLEDVLDGWAGTLLVVSHDRYLLERVCDRQVALLGDGRVRDLPGGVAQYLTLRAAM
                     PPTTGYAVPPAASSATASSGASGGGSGDAGPTQIQQREARKEMARIERALTRLAERED
                     RVHAQMAKEATDHLAVLRLNGDLRAIVDERDDLEMAWLAAAEIVG"
     gene            complement(2540..3478)
                     /locus_tag="PLK64_09285"
     CDS             complement(2540..3478)
                     /locus_tag="PLK64_09285"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPV79982.1"
                     /translation="MSTVSAPPRSVTVRVPAKVNCELRVGPLGADGYHELSTVFMAVG
                     LYDEVTVTRAERWSVTTTGPYAAHVPSGSDNLAMRAARLLARHMRVTRAPVAIRIDKN
                     IPVAGGMAGGSADAAGALVACEHLWGLRMDRDELMELAAELGSDVPFCLAGGIAIGSG
                     RGERLAPVLARGTYHWVFALSSQGLSTPEVFGEFDRLTTDISVPQPEPSPAMMTALRS
                     GDPEALGDALVNDLQEPAFSLRPALRAVLDAGLEFGALGGIISGSGPTVAFLTGSSEH
                     ALDLCVALTASGVVDQVTRAKGPVHGVQILPGPTRE"
     gene            complement(3489..4355)
                     /gene="rsmA"
                     /locus_tag="PLK64_09290"
     CDS             complement(3489..4355)
                     /gene="rsmA"
                     /locus_tag="PLK64_09290"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPV79983.1"
                     /translation="MTSGAALLGAAQIRELAATLGLRPTKAWGQNFVVDANTVRRIVR
                     LAEVGPDDSVVEVGPGLGSLTLALLDSGARVSAVEVDPVLAAALPDTVARHAPERAAY
                     LTVVTADALQVHSLPEPQPTALVANLPYNVSVPVVLSFLERFPTLDRVLVMVQLEVAE
                     RLVAGPGSKAYGVPSVKAAWYAAGRLAGTVPRSVFWPVPNVDSGLVELRRRPVPTTTA
                     SRDEVFAVVDAAFAQRRKTLRAALSGWAGGAAAAETVLRAAGIDPGARGEALPVTAFA
                     AIAAARRDRVTP"
     gene            complement(4352..5488)
                     /locus_tag="PLK64_09295"
     CDS             complement(4352..5488)
                     /locus_tag="PLK64_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HPV79984.1"
                     /translation="MISSLIGRAATVGAVAAIALAGFGAARLDKSIDLNVDGQLTSMH
                     VFGSTVGDALAKSGVTVGEHDLVSPAADQAIEDGSQVVVRYGRKVTVTVDGATTDYWT
                     TATTVDQAMNELGIRALSGATLSVSRSAGLGREGISFTVTNPRQVTITVDGATRAEAT
                     SAATVAELLNALGVTLGANDRVVPAADTAVTEGLAVAVQRVELRTTTATEKVAFKTVK
                     KDDATITKGQTKVQTPGVNGSQVVTFTETWVDGKLEGKTATGTDVTKAATDKVVLVGT
                     KAAPVAASAPVSAGNTSGAGINLANAAMWDRIAQCESGGRWNINTGNGYYGGLQFSYS
                     TWLSVGGADFASRADLASRAEQITVANRLYAQRGLQPWGCRSAA"
     gene            complement(5783..6649)
                     /locus_tag="PLK64_09300"
     CDS             complement(5783..6649)
                     /locus_tag="PLK64_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPV79985.1"
                     /translation="MTGGGTSGAGKAARGIPPAPDPLPIDVVDNHLHLDIARHGEERG
                     DVGETIAAAAAVGVPRMVQIGCDLPGARLTIGMIEEYAALLGGVALHPNEIARFESQA
                     ALADGLAEIERLAAHPRVRVVGETGLDYFRTGPEGQPAQQDAFRWHIDLSKRLGKTLQ
                     IHDRDAHDDVLRILDEEGAPERTVLHCFSGDVGMAQRAVERGLYLSFAGTVTFSSAKG
                     LRDALSVVPLDRVLVETDAPYLTPHPWRGAANAPYLVPVTVRAMAHVLGVAVPTLCQA
                     IAANSERLYGPW"
     gene            complement(6654..>7992)
                     /locus_tag="PLK64_09305"
     CDS             complement(6654..>7992)
                     /locus_tag="PLK64_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPV79986.1"
                     /translation="CDNCGNQMDPAELIEPRSKINGETPRFEETAHFFLDLPALAGAL
                     AEWLDGREATGLWRPNVIKFSKNILDEVRPRPMTRDIDWGIPVPLPGWEDNPNKKLYV
                     WFDAVIGYLSASIEWARRTGDPEAWRRWWNDADAVSYYFQGKDNITFHSQIWPAELLG
                     YAGRGDRGGEPGAAYGVLNLPTEVVASEFMTMEGRQFSTSRGHVIYVRDVLERYGPDP
                     LRYFICAAGPENQDSDFTWAEFVQRNNSELVAGWGNLVNRTASMIHKRFGEIPAAGPL
                     EPIDEELLAAIREGFDTVGGHIERQRFKAGISEVMRLVGLANGYVSATEPFKLKADDQ
                     RERLGTVLHVLAQAVTDLNTLTAPFLPHGANRIHQIMGGTGEFVPMPELREVDDLDSG
                     PGYPVIMGDYGNTPRWESRPVVPGTPIGPPTPVFTKFDPSVVDDEIARMRGDG"
BASE COUNT         1203 a         2744 c         2863 g         1182 t
ORIGIN      
        1 catatgacag acgcagaacc attgtgccca tgacacccgt ttcggcggaa gttctggatc
       61 tctcgaagtc aagactcttg acgctctcgc tacattgagt gggtgggtga ggtgagcgca
      121 cgtgcgccgg gcgaggtcgg ggaccgaccg cccgatgacg tcgaccgtat cgtcgcggct
      181 tggcggcgcg agttgccgca cctggacgtc gagcccctcc tcgtgctctc tcgcatcact
      241 cgactggccc gacggctgga cctggcccga ggggtcgcct tcgccacgca tcacttggaa
      301 agctgggagt tcgacgtcct ctcggcgctg cgccgggccg gacggcccta tcaactgtcc
      361 cccggccaac tcgtcaccga aaccctggtc acctcgggaa cgatgacgaa ccgcgtcgat
      421 cggctagagc aacgcggact tgtccggcgc gggcccgatc ccggtgaccg ccgtggcgtc
      481 aaggtgcgcc tcacccgggt cggccgcgag gcggtcgacg ccgccctgtc cgacctcctg
      541 gcccacgagc gggagttgct cggcgagttg acccccgagg agcaggccac cctcgccggg
      601 atgctgcggc ggctcaacgc cccgctcgag ccctgagcta ctcctgaggt gcacctggtc
      661 acggcgcatc gtcatgcccc gaccctgttc agccgacgat ctcggcagcg gccagccagg
      721 ccatctcgag gtcgtcacgt tcgtcgacga tcgcccgaag atcgccgttg aggcgcagga
      781 ccgcgaggtg atcggtcgcc tccttggcca tctgggcatg cacgcgatcc tcgcgctcgg
      841 ccagccgggt caacgcccgc tcgatccgcg ccatctcctt gcgcgcctcg cgctgttgga
      901 tctgggtcgg cccggcgtcg cccgagcctc cgcccgaagc ccccgacgac gcggtggccg
      961 acgacgcggc gggcggcacg gcatacccgg tggtgggggg catcgcggca cgcagggtga
     1021 ggtattgcgc gaccccgccg ggcaggtcgc ggacccggcc gtcaccgagc agcgcgacct
     1081 gccggtcgca gacccgctcg agcaggtagc ggtcgtgcga cacgacgagc agcgtgcccg
     1141 cccacccgtc gaggacgtcc tcgagcgagg tgagggtctc gatgtccagg tcgttggttg
     1201 gctcgtcgag gagcaggacg ttgggctcgg ccatgagcag tcgcaggatc tgcagccgcc
     1261 gccgctcgcc accggagagg tcgccgacac gggtctgctg ccgctgcccg gtgaagccca
     1321 ggcgggtggc cagttggctc gcggtcacct cgcggtctcc gagcatgatg ctgtgccgga
     1381 ccgcttcgat cgcctcgatg acccggtatg ccgcgagctc gtccagctcg cgcacctcct
     1441 gggtgaggtg ggccagggag accgtcttgc cgaccttgcg acgaccgccc gcgagtgaga
     1501 cgtcgcctga aatcgcttgc agaagagtcg atttcccggc tccgttgact ccgacgatgc
     1561 cgacgcgctc gccgggggcc agttgccagg tcaccttgtc gaggagggtc cgctcgccgt
     1621 agcgcaccgt cgcgtcgaac aggtcgacga cgtccttgcc caggcgccgg gtcgcgaacc
     1681 ggacgagttc taccccgtcg cgcggtggcg gctcgttctc gatgagggcg ttggcggcat
     1741 cgatgcggaa cttcggcttg gtcgtgcggg cgggtgcccc gcggcgcagc caggcgagtt
     1801 ccttgcgcag caggttgtcg cggcgctcgg ccgtcaccgc tgccacacgc gagcgctcgg
     1861 ccttggccag cacgtatgcc gcgtagccgc cgtcgtagct gtggacggcg ccgctgtcga
     1921 cctcccaggt gagggtggcc atggcgtcga ggaaccaccg gtcgtgggtg acggcgacga
     1981 gcgccgtgtc cggacgggtg cggcgggtcg ccaggtgctc ggcgagccag gcgaccccct
     2041 cgacgtcgag atggttggtg ggctcgtcga gcagcagcag gtcggggtcg gccacgagca
     2101 gtgcggccag cgcgagacgc cgtcgctcgc cacccgacag gggtccgacg agggcatccg
     2161 gaccgccgac ggcgagcatg cccaccccac cgagcaaacc gtcgaggacc tcccggaccc
     2221 gggcgtcacc agcccactcg tgctcgggac gatcaccgac cagggcgtgc cggacggtcg
     2281 cggcggggtc gagccggtcg tcctgggcca gcaggccgac ggtgagcccg ccgaccgagg
     2341 tgacccgtcc ggagtcgggc tcctgacggc ctgcgagcac ccgcagcagc gtcgacttgc
     2401 cgccaccgtt gcgcccgacg accccgatcc gctgtccacc gagcacgccg acggtgaggg
     2461 catcgaggac gtgcgcaacg ccgtggacga cggtgacgcg ttcaagtgag acgaggatcc
     2521 ccatgcaggt gtcagcaact cactcgcgcg tcgggccggg aaggatctgg acgccgtgaa
     2581 ccggcccctt ggcgcgggtc acctgatcga cgacccccga ggcggtcagc gcgacgcaca
     2641 ggtccagggc gtgttcggac gacccggtga ggaaggcgac agtcgggccg ctgccgctga
     2701 tgatcccgcc gagggctccg aactccagcc cagcgtcgag gaccgcccga agcgcggggc
     2761 gtagcgagaa ggctggctcc tggaggtcgt tgacgagggc atccccgagg gcctccgggt
     2821 caccggagcg cagggccgtc atcatcgccg gtgatggctc gggttgcggc accgaaatat
     2881 ccgtggtgag ccggtcgaac tccccgaaga cctcgggggt ggacaggccc tgcgagctga
     2941 gcgcgaacac ccagtggtaa gtcccgcgag ccaggaccgg cgcgagacgc tcgccgcgcc
     3001 ccgagccgat ggcgatgcca ccggcgaggc agaacgggac atcgctgccg agctcggcgg
     3061 ccaactccat caactcgtcg cggtccatcc gcaggcccca caagtgctca caagccacga
     3121 gagcgccggc tgcgtcggcg gaccctccgg ccatgccccc cgcgaccggg atgttcttgt
     3181 cgatgcggat cgccaccggc gcgcgggtga cccgcatgtg tcgggcgagc agtcgagccg
     3241 cgcgcatcgc caggttgtcg ctgccggacg ggacgtgggc ggcatacggg ccggtggtcg
     3301 tcaccgacca gcgttcggcg cgggtgaccg tgacctcgtc gtagaggccg accgccatga
     3361 agaccgtcga caactcgtgg tagccgtccg cccccagagg gccgacccgc aactcgcagt
     3421 tgaccttggc aggcacccgc accgtcaccg accggggcgg ggcgctgacg gtactcacgg
     3481 gcaccaccct agggggtcac cctgtctcga cgagcggcgg caatggcggc gaaggccgtc
     3541 acgggcaggg cctcgccgcg ggcaccgggg tcgataccgg cggctcgcag cacggtttcg
     3601 gcggcggcgg cgccaccggc ccacccggac aacgcggcac ggagggtctt gcgacgttgg
     3661 gcgaaggcgg cgtcgacgac ggcgaagacc tcgtcacggg acgcggttgt cgttgggacc
     3721 ggcctgcggc gcagctcgac gagcccggag tcgacgttgg gcaccggcca gaagacgctt
     3781 cgcgggacgg tgccggccag gcgccccgcg gcataccaag cggccttgac gctcggcacg
     3841 ccgtaggcct tgctccccgg cccggccacg agccgttcgg caacctccaa ctggaccatg
     3901 acaagcaccc ggtcgagggt cgggaagcgt tcgaggaagg acaggacgac cggcaccgac
     3961 acgttatagg gcaggttcgc cacgagcgcg gtcggctgcg gctcgggcag ggagtgcacc
     4021 tgcagagcgt cggcggtcac gaccgtgagg tatgccgccc gctccggcgc gtgtcgcgcg
     4081 accgtgtcgg gcaacgcggc tgcgaggacc gggtcgacct cgacggcgct caccctcgcc
     4141 ccgctgtcga gcagggcgag ggtgagtgag ccgagtccgg ggccgacctc gacgacgctg
     4201 tcgtcagggc cgacctcggc caggcgcacg atgcggcgca cggtgttggc gtcgacgacg
     4261 aagttctgtc cccacgcctt ggtcggccgc agcccgaggg tcgcggcgag ttcgcggatc
     4321 tgggcagcac ccagcagcgc tgccccggag gtcacgcggc cgagcggcag ccccagggct
     4381 gcagcccgcg ctgggcgtag aggcggttgg cgacggtgat ctgctccgca cggctggcca
     4441 ggtcggcccg cgaagcgaag tcggcgccgc cgacggacag ccaggtcgag tagctgaact
     4501 gcagccctcc gtagtagccg ttgccggtgt tgatgttcca gcggccaccg gactcacact
     4561 gggcgatccg gtcccacatg gcggcgttgg cgaggttgat gcccgcaccc gaggtgttac
     4621 cggcgctcac cggggcggag gcggcaaccg gggccgcctt ggtgccgacg aggacgacct
     4681 tgtcggtcgc tgccttggtg acgtcggtgc ccgtcgcggt cttgccctca agcttcccgt
     4741 caacccaggt ctccgtgaaa gtgacgacct gcgagccgtt gacgcccggg gtctggacct
     4801 tggtctggcc cttcgtgatc gtcgcgtcgt ccttcttgac ggtcttgaag gccaccttct
     4861 cggtggcagt ggtggtgcgc agctcgaccc gctggacggc gacagccagt ccttcggtga
     4921 cggcggtgtc ggcggcggga acgacgcggt cgttggcgcc gagcgtcacg ccgagcgcat
     4981 tgagcagctc ggcaaccgtc gcggccgagg tcgcctcagc gcgagtggcg ccatcgacgg
     5041 tgatcgtcac ctggcgcggg ttggtcacgg tgaaggaaat cccctcacgg ccgaggccgg
     5101 cggaacgcga gaccgacagc gtcgcgccgg acagcgcgcg gatgccaagc tcgttcatcg
     5161 cctggtccac cgtggtggcc gtcgtccagt agtcggtcgt tgcgccgtcg acggtcacgg
     5221 tgaccttgcg gccataccgc acgacgacct ggctgccgtc ctcgatggcc tggtcagccg
     5281 caggggagac gaggtcgtgc tcgccgaccg tcacaccgct cttggcgagg gcgtcgccga
     5341 cggtcgagcc gaagacgtgc atcgaggtga gctggccgtc gacgttgagg tcgatggact
     5401 tgtcgaggcg agcggcgccg aaaccggcga gggcgatggc tgcgacggca ccgacagtgg
     5461 cggcacggcc gataagactg ctgatcacaa tgctccgaac gttggacgac ccgggcgggc
     5521 acgagacgcc gtgagcggtg aaggcacgtc ccaggtgggc cgtgtcctcc gcctccgagc
     5581 cctgtcacca ggtccagaca cggcgccggg aaaggggtgg tgcctcccgg cgatctgact
     5641 caacggtgac ggacgagcgc ccgaaggtca acttttggta acggaactcc gcttggcaag
     5701 tgacgcaggt cacagaatcg gcgcctcaat aaccccatat gtcacacccg ttgggagtag
     5761 agagatccgt actcgaagcc gctcaccagg gtccgtagag gcgctcggag ttggcggcga
     5821 tcgcttggca gagggtcggc acggcaacgc cgaggacgtg ggccatggcg cggaccgtca
     5881 cagggacgag gtagggcgcg ttggccgccc cccgccacgg gtgcggggtg agatagggcg
     5941 cgtcggtctc gacaaggacc cggtccagcg gcacgaccga aagcgcatcc cgcaggccct
     6001 tggcgctgga gaaggtcacc gtgccggcga aggacagata gaggccgcgt tcgacggccc
     6061 gctgggccat ccccacatcg cccgagaagc agtgcagcac cgtgcgctcc ggcgcaccct
     6121 cctcgtcgag gatccgcagg acgtcatcat gcgcatcgcg gtcgtggatc tgcagggtct
     6181 tgcccaagcg cttggacagg tcgatgtgcc aacggaacgc gtcctgttgc gcaggctgcc
     6241 cctcggggcc ggtccggaag tagtcgagcc cggtctcccc cacgacccgc acccgagggt
     6301 gtgcggccag ccgttcgatc tcggccagcc cgtcagcgag tgccgcctgc gactcgaacc
     6361 gggcaatctc gttggggtgc aaggcaactc cgccgaggag ggcggcatac tcctcgatca
     6421 tcccgatcgt gagccgcgcg ccgggcaggt cgcacccgat ctggaccatc cgcgggaccc
     6481 cgaccgcggc agccgcggcg atggtctcgc cgacgtcgcc gcgttcctca ccgtggcgcg
     6541 cgatgtccag gtggaggtgg ttgtcgacga cgtcgatggg cagcgggtca ggagcgggag
     6601 gtatgccgcg cgcggccttc ccggcgcccg acgtccctcc cccagtcacc ggctcagccg
     6661 tccccgcgca tccgggcgat ctcgtcgtcg acgacgctcg ggtcgaactt cgtgaagacc
     6721 ggggtcggcg gcccgatggg cgtgcctggg accaccgggc gcgactccca ccgcggggtg
     6781 ttgccgtagt cacccatgat gaccgggtag cccggcccac tgtcgaggtc gtcgacctcg
     6841 cgcaactcgg gcatcgggac gaattcgcct gtgccgccca tgatctggtg gatgcggttg
     6901 gcgccgtgcg gcaggaacgg cgcggtcaga gtgttgaggt cagtcacggc ctgagccagg
     6961 acgtgcagca cggtcccgag ccgttcgcgc tggtcgtcgg ccttgagctt gaacggctcg
     7021 gtcgccgaga cgtagccgtt ggccagcccg accagacgca tgacctcgct gatgccggcc
     7081 ttgaaccgct gccgctcgat gtgcccgccg accgtgtcga acccttcgcg gatcgccgcg
     7141 agcaactcct cgtcgatcgg ctccagcggc ccggccgcgg ggatctcgcc gaagcgcttg
     7201 tggatcatcg atgcggtccg gttgacgagg ttgccccacc cggcgacgag ctcagagttg
     7261 ttgcgctgga cgaactcggc ccaggtgaag tcgctgtcct ggttctccgg gccggcggcg
     7321 cagatgaagt agcgcagcgg gtcggggccg tagcgctcga ggacgtcgcg cacatagatg
     7381 acgtggccgc ggctggtcga gaactgccgc ccctccatcg tcatgaactc gctcgccacc
     7441 acctcggtcg gcaggttgag caccccgtat gccgccccag gctcgccacc gcggtctccg
     7501 cgaccggcat acccgagcaa ctcggccggc cagatctggg agtggaaggt gatgttgtcc
     7561 ttgccctgga agtaatagga caccgcgtcg gcgtcgttcc accagcggcg ccaggcctcg
     7621 gggtcaccgg tgcggcgggc ccactcgatc gacgccgaga ggtagccgat gaccgcgtcg
     7681 aaccagacgt agagcttctt gttggggttg tcctcccacc cgggcagcgg caccgggatg
     7741 ccccagtcga tgtcgcgggt catcgggcgc ggacgcacct cgtcgaggat gttcttggag
     7801 aacttgatga cgttgggacg ccacagccca gtcgcctcgc gcccgtcgag ccactcagca
     7861 agcgcgcccg ccagcgccgg caggtcgagg aagaagtggg ctgtctcctc gaagcgcggc
     7921 gtctcaccgt tgatcttgct gcgcggctcg atgagctcgg ccgggtccat ctggttgccg
     7981 cagttgtcgc ac
//
LOCUS       DAOMZY010000145         7940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_8610_length_7940_cov_5.535067, whole genome shotgun sequence.
ACCESSION   DAOMZY010000145 DAOMZY010000000
VERSION     DAOMZY010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7940
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8610_length_7940_cov_5.535067"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..1005
                     /locus_tag="PLK64_09310"
     CDS             190..1005
                     /locus_tag="PLK64_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPV79987.1"
                     /translation="MPSFPTPPPLTSHGPARVIAMCNQKGGVGKTTTTINLGAALAEL
                     GRKVLLVDFDPQGALSVGLGIRPNDLDITIYNLLVERGHDIRDVIQHTRSENLDLIPA
                     NIDLSAAEVQLVGEVAREQILARALRPVMDDYDVIIIDCQPSLGLLTVNALTAAHGVF
                     IPLECEFFAMRGVALLVETIEKITDRLNPRLQVDGILATMYDGRTLHSREVVKSVVDH
                     FGDRVFHTVISRTVKFPDASLAAEPITSYASTSAAAAAYRQLARELIARGGAA"
     gene            1066..1995
                     /locus_tag="PLK64_09315"
     CDS             1066..1995
                     /locus_tag="PLK64_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HPV79988.1"
                     /translation="MTDALLPEAPATPGGVLTRRTSSTPFEVHLDVFEGPFDLLLGLI
                     AKHKLDITEIALARVTDEFIGHIRTIREADEEWDLSQASEFLLVAATLLDLKAARLLP
                     QADPDDDEDLALIEARDLLFARLLQYRAFKDIAATFAEQLATTGRLTPRQAGLEPRFA
                     ALLPELVMGITPEQLAMIAARAMIPKAPPTVALEHLHAAQISVRDQAAILVTRLRVER
                     TCSFRTLVADADSTLVIVARFLALLELYKEGAITFDQAEPLADLAVRWTGSDEGDIAV
                     AAEFDEDDAQLDFDDAGTDTGAASGIPMQGADE"
     gene            1992..2648
                     /gene="scpB"
                     /locus_tag="PLK64_09320"
     CDS             1992..2648
                     /gene="scpB"
                     /locus_tag="PLK64_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HPV79989.1"
                     /translation="MSEDLRVDDEAVPPDDAGSAEETEAAGQVAFDVEAFPGGARSAI
                     EAVLMVVDEPVSEVALATALEVPLADVRAHLEGLEAEYAAANRGFTLRPFGGGWRVYS
                     RSDYAPAVEKFLLDGQQARLTQASLETLAVIAYKQPVSRSRIGAVRGVNVDGVVRTLL
                     SRGLIEERGEAEGGAILYGTTPYFLQRLGLDSLDDLPPLAPYLPDVDMLDEIAEAGRR
                     "
     gene            2645..3697
                     /locus_tag="PLK64_09325"
     CDS             2645..3697
                     /locus_tag="PLK64_09325"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPV79990.1"
                     /translation="MSSAQSGGRGRSGGQSGGGRPSGGGYGGSRGAGGTGGSRGGSGS
                     SGGSGGSGMPPRGPRPEEGGPRKPTGRPTGSGKAPRFAGSTKPSQPTALPDRKVARPA
                     VPVDVHDPDGVRLQKLLAAAGIGSRRACENLIAAGRVEVDGHVVTELGVRIDPLRQSV
                     HVDGERVQLDESRVYLAFNKPLGVVSTMSDELGRPSISDFLGARQDRLFHVGRLDADT
                     EGLLLLTNDGDLAHRLQHPAYGVLKTYLAQVPGPVARDLGKRLRAGIELDDGPVAVDS
                     FKLVDSAPGRALVEVVLHEGRKHVVRRMLAAVGHPVEYLVRTDVGPIRLGDLKVGKTR
                     RLTREEVGKLYTAAGL"
     gene            3746..4840
                     /locus_tag="PLK64_09330"
     CDS             3746..4840
                     /locus_tag="PLK64_09330"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HPV79991.1"
                     /translation="MTTRVLIRGTGLIGTSLGIALTRTGYAVLLADPSPTAERLARDL
                     GAGQLAHESAEQPDLVIIAAPPDVAAGAVAQSLSRWPSAVVTDVASVKGAVVDGARAA
                     GADLTRYVGSHPMAGRERSGAGHARGDLFEGRAWVVVPAPESTESTIQAVTELARAVG
                     SVVTVMDATTHDDAVATMSHLPQIAASLVAAQLRSLDDRALGLAGQGVRDVTRVAASD
                     PRLWTQIIAGNAMSILEVLGRVHADLGELLGALSALADPDAPDDAVGSLEVVNRTITH
                     GNVGHARIPGKHGAGPAAYLPVTALVPDTPGELARLLTDVGEAGVNLEDLRIDHGLGQ
                     PFGLAEIMVVPAAAEHLQQALEAKGWRVLD"
     gene            4840..5556
                     /gene="cmk"
                     /locus_tag="PLK64_09335"
     CDS             4840..5556
                     /gene="cmk"
                     /locus_tag="PLK64_09335"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPV79992.1"
                     /translation="MSASQAAGMPLVVAIDGPSGSGKSSVSRAVATALDAAYLDTGAM
                     YRALTWWCLDQGMDVTDREAVAAVASSAPLEVGMDPDEPLIGVDGNDLTEEVRSVRVT
                     EAVSAIATNLDVRADMRRRQRALIAEGLAARGAVVAEGRDITTVVAPDAPVRILLTAS
                     EDARLARRAQQDFGTADDGAKAATRDRVVARDAADSTVSQFTTAADGVVTIDSSQLDF
                     AQTVAAVLAVVAEARMPSGG"
     gene            5549..6223
                     /locus_tag="PLK64_09340"
     CDS             5549..6223
                     /locus_tag="PLK64_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPV79993.1"
                     /translation="MADVLLAPPAPWRPRAGRWLGIAIFHTAYRGVAIDAHLVPRTGP
                     VVLVANHSGFLDGPLVFSSTPRPVSFLVKQETFHGPLGTLLRGVGQIPVDRGVGDRAA
                     LGAASAVLARGGAVGVFPEGTRGRGDVAQVNQGATWLAVQSGAAVVPVACLGTRRTGD
                     STESMPRVGTRLVAVFGQPFVIPVEAGVPGRERLRRGSELLRERLSAHVTAAAARMGM
                     PLPEDD"
     gene            6343..7773
                     /gene="der"
                     /locus_tag="PLK64_09345"
     CDS             6343..7773
                     /gene="der"
                     /locus_tag="PLK64_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPV79994.1"
                     /translation="MAVERALRAGLADFELDETDLDLLENGGDTAYGDAFAGPLPVAA
                     IIGRPNVGKSTLVNRILGRREAVVEDVPGVTRDRVAYEAEWAGRRFTLVDTGGWEVDA
                     SGIHLRVAEQAEIAIELADAVMFVVDAVVGATDTDEAVVKLLRRSGKPVVLVANKVDD
                     QRFESDATALWGLGLGQPWPVSALHGRGSGDALDALMAVLPERSMVGGAYERGGPRRV
                     ALIGRPNVGKSSLLNKVAGSERVVVDSVAGTTRDPVDELVEIAGKQWRFVDTAGIRRR
                     VHQTRGADFYASLRTQTALEKAEVAVVLIDAGEPISEQDIRVIQQVIDAGRALVVAYN
                     KWDTVDEERRHYLEREIEKELVQIPWAPRVNVSARTGRHLEKLVPALEVALDSWDSRV
                     STARLNAFLGEIVAAHPHPVRGGKQPRILFATQASTRPPRFVLFASGFVEAGYRRFIE
                     RKLRERFGFEGTPIEVSVRVREKRKR"
BASE COUNT         1130 a         2811 c         2812 g         1187 t
ORIGIN      
        1 ggtcgcaccg atcagcgaga ggctgaccga ccgcacgaac gacggggaag aggcgcagct
       61 gtgagcatcg atcgcaacct tcccggcgaa gccgtccaca tggtccccga cctgcgggac
      121 gccaccgaca ccctgctgcc gggcacggaa gacgccggga tcggaggcac cgggccgacc
      181 ggacgcccca tgccgtcctt ccccacgcca cccccgctga ccagccacgg gccggcccgc
      241 gtcatcgcca tgtgcaacca gaagggcggg gtcggcaaga cgaccacgac catcaacctc
      301 ggtgccgccc tcgcggagct cggccgcaag gtgctcctcg tcgacttcga cccgcagggc
      361 gccctgtcgg tgggtctggg gatccggccg aacgacctgg acatcacgat ctacaacctg
      421 ctcgtcgagc gcggccacga catccgcgac gtcatccagc acacccgctc ggagaacctc
      481 gacctcatcc cggccaacat cgacctctcg gccgccgagg tgcaactcgt cggcgaggtg
      541 gcccgcgagc agatcctggc tcgggccttg cgcccggtca tggacgacta cgacgtcatc
      601 atcatcgact gtcagccctc gctcgggctg ctcaccgtca acgcgctgac ggctgcccac
      661 ggggtcttca tccccctgga gtgcgaattc ttcgcgatgc gcggcgtcgc cctgctcgtg
      721 gagaccatcg agaagatcac cgatcgactc aacccccgcc tgcaggtcga cggcatcctc
      781 gccacgatgt atgacgggcg cacgctgcac agccgagagg tcgtcaagtc ggtcgtcgac
      841 cacttcggcg atcgggtctt ccacacggtc atcagccgca ccgtgaagtt ccccgacgcc
      901 agcctggcgg ccgagccgat cacgagctac gcctccacga gtgctgctgc cgcggcatac
      961 cggcaactgg cccgcgagct catcgcccgc ggcggcgcgg cctgagccga ggcatccgcc
     1021 tgcggacgcc gaacccatga ccgtgatacc catgacagtc atcccgtgac cgacgcgctc
     1081 ctgcccgagg cccccgcgac acccggtggg gtgctgaccc ggcgcacctc gagcacgccc
     1141 ttcgaggtcc acttggacgt cttcgagggg cctttcgacc tgctgctggg gctcatcgcc
     1201 aagcacaaac tcgacatcac cgagatcgcc ctggcccgcg tgaccgacga gttcattggg
     1261 cacatccgca ccatccgcga ggccgacgag gagtgggacc tctcgcaggc ctccgagttc
     1321 ctgctggtcg cggccactct gctggatctc aaggcggccc gcctgctgcc gcaggccgac
     1381 cccgacgacg acgaggacct ggcgctcatc gaggcacgcg acctgctgtt cgcgcggctg
     1441 ctgcaatacc gcgcgttcaa ggacatcgcg gcgacctttg ccgagcagtt ggccaccacg
     1501 gggcgcctga cccctcgcca ggctggtcta gagcctcgct tcgcggccct gctgcccgag
     1561 ttggtcatgg ggatcacccc cgagcaattg gcgatgatcg ctgctcgcgc gatgatcccg
     1621 aaggccccgc cgactgtcgc gctggagcat ttgcacgccg cccagatcag cgtgcgcgac
     1681 caggcggcca tcctcgtcac ccggctgagg gtcgagcgga cctgctcgtt ccggaccctc
     1741 gtcgccgatg ccgactccac actggtgatc gtggcgcggt tcctggcgct gcttgagctt
     1801 tacaaagagg gtgcgatcac cttcgaccag gccgagccgt tggccgacct cgccgtgcgc
     1861 tggaccgggt cggacgaggg cgacatcgcg gtcgccgccg agttcgacga ggacgacgcg
     1921 cagttggact tcgacgacgc ggggaccgac accggcgcgg cgagcggcat accgatgcaa
     1981 ggagcagacg aatgagtgag gacctccgcg tggacgacga ggcggtgcca ccggatgacg
     2041 ctgggtcagc cgaggagacc gaggctgccg gccaggtggc cttcgacgtt gaggcgttcc
     2101 ccggcggggc gcggtcggcg atcgaagccg tcctcatggt cgtcgacgag cccgtgagcg
     2161 aggtcgcgtt ggcgactgcc ctcgaagtgc ccctggcgga cgtccgcgcc cacttggagg
     2221 gcctggaagc tgagtatgcc gccgcgaacc gcggcttcac cctgcggccg ttcggcggcg
     2281 ggtggcgggt ctacagccgg tccgactacg ccccggccgt cgagaagttc ctcctcgacg
     2341 ggcagcaggc ccggctgacc caggcgtcgc tggagacgct ggcggtcatc gcctacaagc
     2401 agccggtctc gcgctcgcgc atcggtgccg ttcgcggcgt caacgtcgac ggtgtcgtgc
     2461 gaaccctgtt gtcgcgcggc ctgatcgagg agcgcggcga ggccgagggc ggggcgatcc
     2521 tctacggcac cacgccctat ttcctgcagc gcctcgggct ggactcgctc gatgatctgc
     2581 ccccgttggc gccctacctg cccgacgtgg acatgctgga cgagatcgcg gaagcaggtc
     2641 gacgatgagc tcggcacagt caggtgggcg gggtcgctcc ggcggtcagt ccggtggtgg
     2701 tcgcccctcg ggcgggggct acggcggctc tcgcggggca ggtggcacgg gcggctctcg
     2761 cggcggttcg ggcagttccg gcggctcagg tggatccggt atgccgccgc ggggccctcg
     2821 ccccgaagaa ggcggcccgc gcaaacccac gggacgaccg acgggcagtg gcaaggcccc
     2881 tcggttcgcc ggctcgacca agcccagcca gccgacggcc ctgcccgacc gcaaggtggc
     2941 ccgcccggcc gttccggtgg acgttcacga tcccgatgga gtgcgcctgc agaagctcct
     3001 ggccgccgcg gggatcgggt cacgccgggc ctgcgagaac ctcatcgcgg ccggccgtgt
     3061 cgaggtcgac gggcatgtcg tcaccgagct cggggtccgc atcgaccccc tccgccagtc
     3121 ggtccacgtt gacggcgagc gggtccaact cgacgagtcg cgggtctacc tcgccttcaa
     3181 caaacccctg ggcgtcgtct cgacgatgag cgacgagttg ggtcgcccgt cgatctccga
     3241 cttcctcgga gcccgccagg accggctctt ccacgtcgga cggttggatg ccgacaccga
     3301 gggtctgttg ctgctcacca acgacggcga cctcgcccac cgactgcagc acccggcata
     3361 cggcgtgctc aagacctatc tcgcgcaggt gccgggcccc gtggcccgcg acctggggaa
     3421 gcggctgcgc gccgggatcg agttggacga cgggccggtc gcggtcgact cgttcaaact
     3481 cgtcgactcg gcgccgggcc gcgcgctcgt cgaggtggtc ctgcatgaag ggcgcaagca
     3541 tgtcgtgcgc cggatgcttg ccgccgtcgg gcacccggtc gagtacctgg tgcgcaccga
     3601 tgtcggcccg atccggttgg gtgacctcaa ggtcggcaag acccgccggt tgacccgcga
     3661 agaggtcggc aagctctaca ccgctgcggg tctgtgatgc ggacggccca cgctgccgcg
     3721 ccgaccgctc ggtagcgtga ccgccgtgac aacgcgcgtg ctcatccgcg gcaccgggct
     3781 catcggcacc agcctgggga tcgcgctcac ccgcacgggg tatgccgtgc tcctcgccga
     3841 cccctcgccg accgccgagc ggctggcccg cgacctcggc gccgggcaac tcgcccacga
     3901 gagcgcggag cagcctgatc tcgtcatcat cgctgccccg ccggatgtcg cggccggcgc
     3961 cgtcgcacaa tcgctgtccc gttggccgtc tgcagtcgtc acggacgttg cgtcggtcaa
     4021 gggtgccgtc gtcgatggtg ctcgggctgc gggcgccgat ctcacccgct acgtcgggtc
     4081 ccacccgatg gccggccgcg agcgctccgg cgccgggcac gcccgcgggg accttttcga
     4141 gggccgggcc tgggtggtcg tgcctgcccc ggagtcgacc gagtcgacaa tccaggccgt
     4201 gacggagttg gcgcgggccg tcgggtcggt ggtcaccgtc atggacgcca ccacccacga
     4261 cgacgctgtg gcaacgatgt cgcacctccc gcagatcgca gcgagtctcg ttgcagccca
     4321 actgcgttca ctggacgacc gggcgctggg cctggccggg cagggcgtcc gcgacgtcac
     4381 ccgggtcgcc gcgagcgacc cgcggttgtg gacgcagatc atcgccggca acgccatgtc
     4441 gatcctcgaa gtcctcgggc gggtccacgc cgacctgggc gagttgctgg gcgccctgag
     4501 cgcgctcgcc gaccccgacg ctcccgacga cgcggtcggc agccttgaag tggtcaaccg
     4561 caccatcacg cacggcaacg tcggccacgc gcggatccct ggcaagcatg gagctgggcc
     4621 cgcggcatac ctgccggtca cggcgctcgt ccccgacacg cccggcgagc tcgcgcggct
     4681 gctcacggac gtcggcgagg cgggggtcaa cctcgaagac ctgcgcatcg accacggcct
     4741 cgggcagccc ttcgggctcg ccgagatcat ggttgtcccc gccgccgccg agcacctcca
     4801 gcaggcgctg gaggccaagg gctggcgggt gctcgactga tgagcgcctc acaagcggcg
     4861 ggtatgccgc tcgtcgtcgc cattgacggc ccgtccggct cgggcaagtc gtcggtgtcg
     4921 cgggcggtcg ccacggcgct ggatgccgcc tatctcgaca ccggtgccat gtaccgcgcg
     4981 ctcacctggt ggtgcctcga ccaggggatg gacgtcaccg atcgggaggc cgtcgccgcc
     5041 gtggcgtcga gcgcaccgct ggaggtgggg atggacccgg atgagcctct gatcggggtc
     5101 gacgggaacg acctcaccga ggaggttcgc tcggtccggg tcaccgaggc cgtctcggcc
     5161 atcgcgacca acctcgacgt gcgggccgac atgcgccggc ggcaacgggc cctcatcgcc
     5221 gaaggcctcg cggcccgggg cgccgtcgtc gctgaaggcc gcgacatcac cacggtggtc
     5281 gccccggacg cgccggtgcg gatcctcctc accgcgagcg aggacgcccg actggcccgc
     5341 cgcgcccaac aagatttcgg cactgcagac gacggggcca aggcagccac ccgcgatcgg
     5401 gtggtggcgc gcgatgccgc cgactcgacg gtgtcgcagt tcaccaccgc cgccgacggc
     5461 gtggtgacga tcgacagttc ccaactcgat ttcgcgcaga ccgtggccgc agtgcttgcg
     5521 gtggtcgccg aggcccggat gccttccggt ggctgacgtc ctcctggcgc ctcccgcgcc
     5581 gtggcggccc cgcgccggac ggtggctggg catcgcgatt ttccacacgg cataccgagg
     5641 ggtggcgatc gacgcgcacc tcgtgccgcg cacggggccg gtcgtcctgg tcgccaacca
     5701 cagtgggttc ctcgacgggc cgctcgtctt cagtagcacc ccgcggccgg tgtcgttcct
     5761 cgtcaagcag gagaccttcc acggcccgct agggaccttg ctgcgcgggg tcgggcagat
     5821 ccccgtcgat cggggtgtgg gggaccgtgc ggccctcgga gcggccagcg cggtgctggc
     5881 gcgcggggga gcggtcgggg tgttccccga aggcacccgc ggtcgcggag acgtcgccca
     5941 ggtcaatcag ggcgcgacat ggctcgcggt gcagagcgga gccgccgtcg tccctgtggc
     6001 gtgtctcggc acgcggcgta cgggggacag caccgagtcg atgccccggg tcggcacccg
     6061 attggtcgct gtcttcggcc aaccgttcgt catccccgtc gaggccgggg tgcctggtcg
     6121 ggagcggctg cgcaggggct cggaactgct gcgcgagcgc ctgtcggccc atgtcaccgc
     6181 tgcggccgcc cggatgggta tgccgctccc cgaggacgac tgaaccgcgc gtcgtcggcg
     6241 aggtgggaga atccgggggt gaacccgcag aaccccaccg accccgagct tgagctcgca
     6301 ccctccgaat cggccgcgac gactgcgacg acccctgacg agctggcagt cgagcgcgcc
     6361 ctgcgtgccg ggctggccga ctttgagctc gacgagaccg acctggacct gcttgagaac
     6421 ggcggcgaca cggcatacgg ggatgccttt gccgggcccc tcccggtggc ggcgatcatc
     6481 gggcgcccca acgtcggcaa gtccaccctc gtcaaccgga tcctcgggcg ccgtgaggcg
     6541 gtcgtcgagg acgtgccggg agtgacgcgg gaccgggtgg cttatgaggc cgagtgggcc
     6601 gggcggcgat tcaccctcgt cgacaccggc ggctgggagg tcgacgcctc cggcatccac
     6661 ctgcgggtcg ccgagcaggc cgagatcgcc atcgagttgg ccgacgccgt gatgtttgtc
     6721 gttgacgccg tcgttggtgc gaccgacacc gacgaagccg tcgtcaagtt gctgcgccgc
     6781 tcgggcaagc cggtcgtcct tgtggccaac aaggtcgacg accagcgatt cgagtcggac
     6841 gcgacggccc tgtgggggct cgggttgggg cagccgtggc cggtctccgc cctgcacggg
     6901 cgggggagcg gcgacgcgct cgacgcgctc atggccgtgt tgcccgagcg ctcgatggtc
     6961 ggcggggcgt atgagcgtgg cggtccgcgc cgggtagccc tcatcggtcg ccccaacgtc
     7021 ggcaagtcgt cgttgctcaa caaggtggcc gggtcggagc gggtcgtcgt cgactcggtg
     7081 gccgggacga cgcgggaccc ggtcgatgag ttggtcgaga tcgccgggaa gcagtggcgg
     7141 ttcgtcgaca cggcggggat ccggcggcgg gtccaccaga cccgcggcgc cgacttctat
     7201 gcctcgctgc gcacccagac ggcgctggag aaggccgagg tggctgtcgt gctcatcgac
     7261 gccggtgagc cgatctctga gcaggacatc cgggtcatcc agcaggtcat cgacgccggt
     7321 cgggccctgg tcgtggccta caacaagtgg gacacggtcg atgaagaacg gcgccactac
     7381 ctcgagcgcg agatcgagaa ggagctcgtc cagatcccgt gggccccgcg cgtcaatgtg
     7441 tcggcacgca ccggccggca cctggagaag ctcgtcccgg cgctcgaagt tgctctcgac
     7501 tcgtgggact cacgggtgtc gacggctcgg ctcaacgcgt tcctcgggga gatcgtcgcg
     7561 gctcacccgc acccggtgcg tggcggcaag cagccccgga tcctcttcgc cacgcaggcc
     7621 tcgacccggc cgccgcggtt cgtgctcttc gcgtcgggct tcgtcgaggc cggctatcgc
     7681 cggttcatcg agcgcaagtt gcgcgagcgg ttcggcttcg aggggacgcc catcgaggtg
     7741 tccgtgcggg tccgcgagaa gcgcaagcgc taggagggtg ccggggcggc tgccggttgc
     7801 gcggcatacg gattgggagc cccggtatgc cgtgcggtag ggtttcgttc gctctcgtgg
     7861 cgcccttttg gggcggtggg agggcagcca cgggctgtgg cgcagcttgg tagcgcactt
     7921 gactgggggt caaggggtcg
//
LOCUS       DAOMZY010000146         7837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_8741_length_7837_cov_6.071190, whole genome shotgun sequence.
ACCESSION   DAOMZY010000146 DAOMZY010000000
VERSION     DAOMZY010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7837
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8741_length_7837_cov_6.071190"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..1635)
                     /locus_tag="PLK64_09350"
     CDS             complement(136..1635)
                     /locus_tag="PLK64_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPV79995.1"
                     /translation="MSVPTHTTPPPPIPQEVRDWVPATRTLLVGGSMVEPRGETWDVF
                     NPATEAVIATVSGASADQVDAAVLAARNAFPAWAALSGAERGAYIHRFADALEAAADR
                     LLPSIVNEVGTPVSLAEYLQVKMAVQEHLRWAADAAATDRTVHLGRYETPWPTMSDVV
                     HEPVGVVAAITGYNYPLNLAIFKFGAALAAGCTVVLLPSPRTPLTTLLLGDLIQESGL
                     PAGVMNVIIGGADVGRQLSSHPDVDRVSFTGSDGVGALIMEQAARNLAGVTLELGGKS
                     PSIVMPDVDVAKVAVEMHLRWSRNAGQGCAALARLLVHESRFDEFVEAGASAFDQMVV
                     GDPWDRATNIGPLIRPDHQARVQSFITDSLAVGGRKLLEVTKPLPEKGWYVNPVLLGG
                     LDHSARACQEEIFGPIAVLVPFKDTEDAIRLANDTKYGLAANVWCNDPIEARRVADRV
                     RAGTVWINGGGGMRPDAPFGGFGRSGIGRELGEWGMREYLEVKHVQWRL"
     assembly_gap    1674..1773
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1774..3341)
                     /locus_tag="PLK64_09355"
     CDS             complement(<1774..3341)
                     /locus_tag="PLK64_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPV79996.1"
                     /translation="MSQPISHPMTYRDLVPTFPDPTTWTLDQVLRHHAQVRPDALALE
                     CPEESVTLTYAAGLADAERLASGMLAAGARPGDRVVIMAANSSRFVRSWWATAVSSLV
                     EVPVNTNYEGEFLRHQVTVVQPRFAVIDDTYAERFVAIKEQVRVIEEFWVIDTGAGIH
                     DDAIDLLREHGWVAGDWEELLQAEPVALTAPSPQDLGAIFFTSGTTGPSKGVAMPNSQ
                     LYFFAQEVACFTRLTPEDVYLTTTPLFHGNAQFMAAYPALLAGGRAVVRPKFSASRWI
                     DHVRDSGVTVTNFVGVMMDFAWKQAPRPDDRNNHLRCVYAAPTASSIVEEFKERYGIE
                     AFVDAFGLTETSAPIISPYGVPRPAGAAGLQASDWFDIRLVDPETDREVAVGEIGELV
                     VRPVHPWTCSMGYYGMPEKTVEAWRNLWFHTGDALRKDADGWFYFVDRYKDALRRRGE
                     NISSYEVEQAILGHPAVTECAVIGVPADVEAGEDEVLAAVVVDGPVDPAEILAWCAGR
                     IPAFAVPRFMRVVDA"
     gene            complement(3338..4393)
                     /locus_tag="PLK64_09360"
     CDS             complement(3338..4393)
                     /locus_tag="PLK64_09360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HPV79997.1"
                     /translation="MVNVGLLISDIPRSTPSKQHFSDLLRFVEAAQRNGFTYLAMGQH
                     FLYGDLRWLQPVPLFARLAAEVDPDFKLVTQIIIAPLYHPVLLAEEIATLDIVTEGRL
                     IFGAGGGYRPEEFTFLDVPYKERGARFDESLELMTKLWTQETVTHHGRFWNLDGVQPH
                     IQPVQQPHPPIWIGAHAEAGVRRAAKYGDAYACPPETPVREVARRYAIVRDGFAARGK
                     AFTSQPLRRNIQVGDTREEAMVEYARVAQGRYLTYLNKGLDVMANTDLENEFAKAVSS
                     HAVIGTDDQVAAELCDLVQTLPVDPILLRPTWPTMSSDEMIAALDRLGRTVVPALRAI
                     TPRTDIDEALLAPQEGQ"
     gene            complement(4427..5194)
                     /locus_tag="PLK64_09365"
     CDS             complement(4427..5194)
                     /locus_tag="PLK64_09365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPV79998.1"
                     /translation="MTADENPGRGVVVTGAARGMGKAIAQRFAASGHVVIGVDRDADA
                     LAATFAELGAPHIGIAGDVCDEELLARVCEQAVEAGGGLRCFVANAGIIGPGPSTEQP
                     VAQWDGLLGVNLTGVFLGARHAARVMRPGASIVATSSICGSLGFGGRAAYGASKAGIN
                     GLVWALAVEWGPLGIRVNAVAPGAIRTELAAAMIATGRVDESVYEARIPMGRRGRPEE
                     VAAAVHFLASDDASYVSGVVLPVDGAWAINGLPAQAG"
     gene            complement(5191..>5686)
                     /locus_tag="PLK64_09370"
     CDS             complement(5191..>5686)
                     /locus_tag="PLK64_09370"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPV79999.1"
                     /translation="TRAAAHTGPDTDTAIVAARSAQLYYRYARAVDDHDHEALRAMVT
                     DDVNMTRADGTREGVEAFLDVYRAFTAQAIPSSKHVVTNILATRAAAGEIHTQAYFEA
                     SMFGVESTRVIVGHYDDIHVERDGRLLLAHKIISVDRVLTLPAAEASYVNVGSTAAAD
                     GADA"
     assembly_gap    5687..5786
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5816..6580)
                     /locus_tag="PLK64_09375"
     CDS             complement(5816..6580)
                     /locus_tag="PLK64_09375"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPV80000.1"
                     /translation="MAVVVGGGSGMGRAISERLASEGAIVYVADAREQAAYEVASAIT
                     ASGSAAIPYAAIPHVVDATSVDALRALFERVEADHGVLHVFHHQVGAPGAGGIDFSEN
                     EFDDAVAVNIKSATFGASLAWPLLSRAEGKASVTFTASTAALVGSPFSPLYSLTKGAL
                     VAFARSLALVGAPVGIRVNVVAPGPVDTPMLPTFFGREPGASIADLMDSFLATVPLGR
                     PGTPGEVAGTVAFLASDDAGFITGITIPVDGGLTAR"
     gene            complement(6667..7185)
                     /locus_tag="PLK64_09380"
     CDS             complement(6667..7185)
                     /locus_tag="PLK64_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80001.1"
                     /translation="MGRVGIDDLEALQGLGLRHEERAELLATQTECTFVFSSEAGWPS
                     GVVMTYIHIDGVFWLTAVEGRGQTKGIAKDPRATIVVSNRGTDLPGRQMAAYRCVVTV
                     HRDRRTKAWFIDEFTKRLQPGNEAPWMKLLDSPNRVIFKAQPVALAVSHDSRKIPGGG
                     RNTTGSSAGGGE"
     gene            7353..>7837
                     /locus_tag="PLK64_09385"
     CDS             7353..>7837
                     /locus_tag="PLK64_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005047764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPV80002.1"
                     /translation="MSNAVIVEAVRTPMGRGKAGGALADLHPVDLLAQTLTALVARTG
                     IDPGIVDDVIIGCVGQNLEQSATPGRQAWLAAGFPEHVPSVTIERKCGSGQQAIEFAA
                     AGIVAGLYDIVIAGGVESMSRVPIMSARMGADPHGPGVRARYPGLVPQGVSAELVAHR
                     WG"
BASE COUNT         1140 a         2621 c         2725 g         1151 t
ORIGIN      
        1 acatcgtgcc cagttcgagc acgcgtatgc cgtgcagcgg gccttcgcgt ctcgtcgcac
       61 ctgccgccgg gctcgcgtcc acgggcgcgc ccccaaccga ggttgcgtcc tcagacaccg
      121 acgggccgtc cgacatcaga gccgccactg gacgtgcttg acctcgaggt attcgcgcat
      181 gccccattca ccgagctcgc gcccgatgcc ggagcggccg aacccgccga agggggcgtc
      241 cgggcgcatc ccgccgccgc cgttgatcca gaccgtgccg gcgcgcaccc ggtcggcgac
      301 ccggcgggcc tcgatcgggt cgttgcacca gacgttggca gccaggccgt acttcgtgtc
      361 gttggccagc cggatcgcgt cctcggtgtc cttgaacggc accaagaccg cgatcgggcc
      421 gaagatctcc tcctggcagg cccgcgcact gtggtcgagc ccgccgagga gcacggggtt
      481 gacgtaccaa cccttctccg gcagcggctt ggtcacctcg agcagtttgc gcccaccaac
      541 agcgagcgag tcggtgatga aggactgcac ccgagcctgg tgatccggac ggatgagcgg
      601 gccgatgttg gtcgcgcggt cccacgggtc gccgacgacc atctggtcga aggccgacgc
      661 ccccgcttcg acgaactcgt cgaatcggct ctcgtggacg agcaagcgcg ccagcgccgc
      721 acaaccctgg ccggcgttgc gcgaccagcg caggtgcatc tcgaccgcga ccttggcgac
      781 gtcgacatcg ggcatgacga tgctcggcga cttgccgccg agctccagcg tgaccccggc
      841 caggttgcgc gccgcctgct ccatgatgag cgcgccgacg ccgtcggacc cggtgaacga
      901 cacccggtcg acgtcggggt gcgaggacaa ctgccgtccc acgtcggcgc cgccgatgat
      961 gacgttcatg acgcccgcgg gcagccccga ctcctggatg aggtcgccga gcaacagggt
     1021 cgtcagcggc gtgcgcggcg acggcagcag cacaacggtg caccccgcgg ccagcgccgc
     1081 accgaacttg aagatcgcca ggttgagcgg gtagttgtag cccgtgatcg ccgcgacgac
     1141 cccgaccggc tcatgcacga cgtcgctcat cgtcggccac ggcgtctcat aacgccccag
     1201 gtggacggtg cgatcggtcg ccgccgcgtc ggccgcccag cgcaggtgct cctgcacggc
     1261 catcttgacc tgcaggtatt ccgcgagcga gaccggggtc ccgacctcgt tgacgatcga
     1321 gggcagcagt cggtctgcgg cggcttcgag cgcgtcggcg aagcggtgga tgtaggcgcc
     1381 gcgctcggca cccgacagcg ccgcccacgc cggaaaggcg ttccgcgcag ccagcacggc
     1441 ggcgtcgacc tgatcggcgc tcgccccgct gacggtcgcg atgacggcct cggtcgccgg
     1501 gttgaagacg tcccacgtct cgccgcgcgg ctcgaccatc gagccgccga cgagcagtgt
     1561 gcgggtcgcc gggacccagt cgcgcacttc ctgcgggatc ggcggcggcg gagtggtgtg
     1621 ggtgggcacg ctcatgcgtg ggcctcctcg gtctgggtcg cgggagcagg ggtnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcgtcga cgacccgcat gaaccgcggg
     1801 acggcgaagg cggggatccg gccggcgcac cacgcgagga tctcggcggg gtcgacgggg
     1861 ccatcaacga cgaccgccgc gagcacctcg tcctcgccgg cctcgacgtc ggcgggcacc
     1921 ccgatgacgg cgcactcagt gaccgccggg tggccgagga tggcctgctc gacctcgtag
     1981 gagctgatgt tctccccgcg ccgacgcagc gcgtccttat agcggtcgac gaagtagaac
     2041 cacccgtcgg cgtccttgcg cagcgcgtca ccagtgtgga accacaggtt gcgccaggcc
     2101 tccacggtct tctcgggcat cccgtaatag cccatgctgc aggtccacgg atgcaccggc
     2161 cgcacgacca actcgccgat ctcgccgacc gccacctcgc ggtcggtctc ggggtcgacc
     2221 aggcggatgt cgaaccagtc cgacgcctgc agccccgcgg cacccgcagg ccgtggcacg
     2281 ccgtagggcg agatgatcgg cgcgctcgtc tcggtcagcc cgaacgcgtc gacgaacgcc
     2341 tcgatgccat accgctcctt gaactcttcg acgatcgagg aggcggtggg ggcggcatac
     2401 acacagcgca ggtgattgtt gcggtcgtcc gggcgcggcg cctgcttcca cgcgaagtcc
     2461 atcatcacgc cgacgaagtt ggtgaccgtc acgcccgagt cgcgcacgtg gtcgatccac
     2521 cggctcgccg agaacttcgg acgcaccacc gcgcgccctc ccgcgagcaa ggcggggtat
     2581 gccgccatga actgcgcgtt cccgtggaaa agcggtgtcg tcgtgaggta gacgtcctcg
     2641 ggcgtgaggc gggtgaagca ggccacctcc tgggcgaaga agtagagctg agagttgggc
     2701 atcgcgacgc ccttgctcgg cccggtcgtg cctgaggtga agaagatcgc gccgaggtcc
     2761 tgcggcgagg gcgcggtcag cgcgacgggc tcggcctgca gtagctcctc ccaatcgccg
     2821 gccacccagc cgtgctcgcg cagcaggtcg atggcgtcgt catggatccc ggccccggtg
     2881 tcgatgaccc agaactcctc aatgacccgc acctgctcct tgatggcgac gaagcgctcg
     2941 gcatacgtgt catcgatgac ggcgaagcgt ggctggacga cggtcacctg gtgacgcagg
     3001 aactcgccct catagttggt gttgaccggc acctcgacga ggctggacac ggccgtagcc
     3061 caccaactgc gcacgaaccg cgaggagttg gcggccatga tgacgacccg atcgcccggc
     3121 cgggctccgg ccgcgagcat ccccgaagcg agccgttccg cgtcggccag ccccgcggca
     3181 tacgtgaggg tgacggactc ctccgggcac tccagggcga gcgcgtcggg ccgcacctgc
     3241 gcgtggtgac gcagcacctg gtcgagcgtc caggtcgtgg ggtcggggaa ggtcgggacg
     3301 aggtcccgat aggtcatcgg gtgactgatc ggctggctca ctggccctcc tgcggggcga
     3361 gcagcgcctc gtcgatgtcg gtgcgggggg tgatcgcgcg cagcgcgggg acgacggtgc
     3421 gaccgagccg gtccagggcc gcgatcatct cgtcgctgga catcgtcggc caggtcgggc
     3481 gcagcaggat cgggtcgacg ggcaacgtct ggacgaggtc gcacaactcg gcggccacct
     3541 gatcgtcggt cccgatgacg gcgtgcgacg acacggcctt ggcgaactcg ttctccaggt
     3601 cggtgttggc catgacgtcc aggcccttgt tgagataggt gaggtaacgc ccctgggcga
     3661 cccgggcgta ttccaccatc gcctcctcac gggtgtcgcc tacctggatg ttgcgccgca
     3721 gcggctgcga ggtgaaggcc ttaccgcgcg cggcgaatcc gtcgcggacg atcgcatagc
     3781 ggcgagcgac ctcgcgcacc ggggtctcgg gcgggcaggc ataggcgtcg ccatactttg
     3841 ccgcgcgacg gacgccggcc tcggcatggg ccccgatcca gatcggcggg tgcggctgct
     3901 ggacgggctg gatgtgcggc tggacgccgt cgaggttcca gaaccggccg tggtgggtga
     3961 cggtctcctg ggtccagagc ttggtcatca gctcgagcga ctcgtcgaag cgggcgccgc
     4021 gctccttgta aggaacgtcg aggaaggtga actcctcggg ccgatatccg ccacccgccc
     4081 cgaagatcag ccgaccttcg gtgacgatgt cgagggttgc gatctcctcg gcaagcagca
     4141 ccgggtgata gagcggcgcg atgatgatct gggtgacaag cttgaaatcg gggtcgacct
     4201 cggccgccag ccgggcgaac aagggcaccg gctgcagcca gcgcaggtcg ccgtagagga
     4261 agtgctgccc catcgccaga taggtgaacc cgttgcgttg ggccgcctcg acgaagcgca
     4321 gcaggtcgct gaagtgctgc ttggacggcg tcgaccgggg gatgtcgctg atgagcagcc
     4381 cgacgttgac catgaggttc ctcttccttc tgcttcgttc cgcggctcag ccggcctgcg
     4441 ccggaaggcc gttgatcgcc cacgcgccgt cgaccggcag aacgaccccc gacacatagc
     4501 ttgcgtcgtc cgaggcgagg aagtgcacgg cggccgcgac ctcctcggga cgaccgcgcc
     4561 gccccatggg gatgcgcgcc tcgtaaacac tctcgtcgac ccgaccggtc gcgatcatcg
     4621 cggcggcgag ctcggtgcgg atcgcgccgg gggccaccgc gttgacgcgg atcccgagcg
     4681 gcccccactc caccgccagc gcccacacca gtccgttgat ccccgccttg gacgcaccgt
     4741 atgccgcgcg cccgccgaag cccagcgacc cacagatcga gctggtcgcc acgatgctgg
     4801 cgccgggtcg catgacccga gcggcatgcc gagcgccgag gaagacgccc gtgaggttga
     4861 cgccgagcag gccgtcccat tgggccaccg gttgctcggt gctcggtccg gggccgatga
     4921 tgccggcgtt ggcgacgaag cagcgcagcc cgccaccggc ctcgaccgcc tgctcgcaga
     4981 cccgcgcgag cagctcctcg tcacagacgt cgccagcgat gccgatgtga ggggcgccga
     5041 gctcggcgaa ggtcgcggcg agcgcgtcgg cgtcgcggtc gaccccgatg acgacatggc
     5101 cggacgcggc aaagcgctgt gcgatcgcct tgcccatgcc gcgggcggcg ccggtgacga
     5161 cgaccccccg cccggggttc tcgtcggcgg tcatgcgtcc gcgccgtccg cggccgccgt
     5221 cgagccgacg ttgacgtagg acgcctcggc tgccggcagg gtgaggacgc ggtcgacgga
     5281 gatgatcttg tgggccagca gcagtcggcc atcgcgctcg acgtggatgt cgtcgtagtg
     5341 cccgacgatc acccgcgtcg actcgactcc gaacatgctg gcctcgaaat acgcctgggt
     5401 gtggatctca ccggccgccg cccgagtcgc gaggatgttg gtgacgacgt gtttgctcga
     5461 cgggatcgcc tgcgcggtga aggcccgata gacgtcgagg aacgcctcga cgccctcacg
     5521 ggtgccatcg gcgcgggtca tgttcacgtc gtcggtgacc atcgctcgca gcgcctcgtg
     5581 gtcgtggtca tcgaccgctc gggcatagcg gtagtagagc tgcgcggatc gcgcggcgac
     5641 gatcgcggtg tcggtgtcgg gacccgtgtg tgcggccgcg cgggtgnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnggtg ctgtcctgtg gggtgctgtg gtcggtcatc
     5821 tcgcggtcaa ccctccgtcg acggggatgg tgatgccagt gatgaaacct gcatcgtcac
     5881 tagcgaggaa ggccaccgtt ccggcgacct ccccgggggt tcccggccgg ccgagaggca
     5941 cggtggcgag gaaggagtcc atgaggtcgg cgatgctcgc gccgggctcg cggccgaaga
     6001 aggtcggcag catcggggtg tcgaccggcc cgggcgcgac gacgttgacc cggatcccga
     6061 ccggggcccc gacgagcgcg agtgagcggg cgaaggccac gagcgcgccc ttggtcaacg
     6121 agtagagcgg gctgaacggg ctgccgacga gggccgcggt cgaggccgtg aaggtcaccg
     6181 acgccttgcc ttcggcgcgc gagagcagcg gccaggccag cgacgcaccg aaggtcgccg
     6241 acttgatgtt aacggcgaca gcatcgtcga actcgttctc ggagaagtca attcctccgg
     6301 cgccaggcgc gccgacctgg tggtggaaca cgtgcagaac gccgtggtcg gcctcgaccc
     6361 gctcgaaaag cgcgcggagc gcgtcgacgg aggtggcgtc cacgacatgg gggatcgcgg
     6421 catacgggat ggcagcgctg ccgctcgccg tgatggcgct ggcgacctcg tatgccgcct
     6481 gctcgcgcgc atcggcaacg tagacgatcg cgccctcgga ggccaggcgc tcgctgatcg
     6541 cgcggcccat gccggaaccg ccgccgacga ccacggccac gcggccgacc aaccgtcggc
     6601 acgggggtcc gctggtgggg ctgggcgtag ggcgggtcgc cgtcagggcc gggtcctcgg
     6661 gcgcgctcac tcgccgcccc cggcgctgct gcccgtcgtg ttgcggccgc cgccggggat
     6721 cttgcgggag tcgtggctga ccgcgagcgc gaccggctgg gccttgaaga tgacccggtt
     6781 ggggctgtcg agcagcttca tccacggggc ttcgttgccc ggctgcagtc gcttggtgaa
     6841 ctcgtcgatg aaccacgcct tggtgcgccg gtcgcggtgg acggtgacga cgcagcggta
     6901 tgccgccatc tgtcgccccg gcaggtcggt ccccctgttg ctgacgacga tcgtcgcgcg
     6961 cgggtccttg gcgatgccct tggtctgacc gcgaccttcg accgcggtga gccagaagac
     7021 gccgtcgatg tggatgtagg tcatgacaac cccgctgggc cacccggcct cactggagaa
     7081 gacgaaggtg cactcggtct gcgtcgccag caactcggcg cgctcctcgt gccgcagccc
     7141 cagaccctgg agggcctcga ggtcatcgat cccgacccgt cccatgtccg cacctcctcc
     7201 acgttgttct ctagccgggg tgatgcctag cccgcgcttt gatgatattc atgttcatga
     7261 tttgcggtca agggctcccc gtggccaaga gcgattgtgt atgctaacga taatcagaga
     7321 tcgggcccgt gcgggctccc gaggaggtcg tcgtgagcaa cgcggtcatc gtcgaagcgg
     7381 tgcggacgcc catgggtcgc ggcaaggcgg gcggcgccct cgccgacctg catccggttg
     7441 acctgctcgc ccagactctc acggcgttgg tcgctcggac gggcatcgac cccggcatcg
     7501 tcgacgacgt catcatcggc tgcgtcgggc agaacctcga gcagtcggcc accccgggcc
     7561 ggcaggcgtg gctggcggcc gggttccccg agcacgtccc atcggtgacc atcgagcgca
     7621 agtgcggctc cgggcagcag gcgatcgagt tcgccgcggc agggatcgtc gcggggctct
     7681 atgacatcgt catcgccggc ggggtcgagt cgatgagccg ggtgccgatc atgtccgcgc
     7741 ggatgggcgc cgacccgcac ggccccggcg tccgggcgcg ctaccccggc ctcgtcccgc
     7801 agggggtgtc cgccgagctc gtcgcgcatc gctgggg
//
LOCUS       DAOMZY010000147         7821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_8776_length_7821_cov_6.935359, whole genome shotgun sequence.
ACCESSION   DAOMZY010000147 DAOMZY010000000
VERSION     DAOMZY010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7821
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8776_length_7821_cov_6.935359"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="PLK64_09390"
     CDS             complement(<1..1082)
                     /locus_tag="PLK64_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPV80003.1"
                     /translation="MTASFDPALPVTGPVTGLISTPAIGLNRPHHDGSALYVSTETPR
                     LGAVVSLWLRVPAVATAPGEAPFDAAYVRVLRDGEPHFVTAVRDAERSTEREVWWRAE
                     VDCRNPVTSYRWLLSGGAIGWLWVNGAGSHLRDVPDAANFRIVAVDTPLPQWPLDAVV
                     YQVFPDRFARSAAADERETPDWAVEAAWSDPVETRSRAGGSRQLYGGDLDGVAEHLDH
                     IAALGADVVYLTPFFPGRSNHRYDAHTFDAVDPLLGGDAALRRLTDAAHARGIRVLGD
                     FTSNHTGVTHEWFEAARADESAPERAFYFFGPGQEYAAWLGVPSLPKLDHSSPELRRR
                     LFEDPRGVIQRWLGPDGGLDGWRVDVA"
     gene            complement(1079..1999)
                     /locus_tag="PLK64_09395"
     CDS             complement(1079..1999)
                     /locus_tag="PLK64_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPV80004.1"
                     /translation="MTATVSTPSTPPAPSTPTHAFDAPPTAKRRDPWQVVRYLAIGAA
                     LIFALFPIWFIVAAAVNPSGNLNTTQLIPDAFGLGNYTDLTASRPFWSWYKNTLIIAG
                     IGSIAAVFIGVCAAYAFSRLRFQGRRPGLMTLLLIQMFPSLLTFVALFRIFTELGDVI
                     PQFGLNTSLGLILAYLGGAMGGNVWLLKGYFDTVPKELDEAAKVDGASHARVFFTITL
                     PLITPILVTVGMIQFIGLFNEFLLASVFLKDVDAQTLGVGLNALLTADRNKYFGAFCF
                     GAVLASAPIVLIYLSFQRQLIGGLTAGSVK"
     gene            complement(1999..3540)
                     /locus_tag="PLK64_09400"
     CDS             complement(1999..3540)
                     /locus_tag="PLK64_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPV80005.1"
                     /translation="MTKSPWIVVKWVAIAAALALLVWIAGKFAAAGSLWAVVGVAFIA
                     MCVLAIYGTTRAVPLKYLFPGLFFLVALQIWPIIFTIATSFTNYGDGHMGTKEESVKY
                     LIAQSVREVEGAPRYAMSVAVPTGADVTTGTITLLLTDPKDGATYAGTPEGLAPLTDG
                     VEKSPTGKVTKANGFTILNAREVNARSADLSALAVPTEGGGIKTSGLSEAFVGKASMQ
                     YDAAADRMIDTTTGKRYLPQNALWVPEDGQGQSLTSGWQENVGLRNYTEALTNETLRT
                     GFLKILVWNLIFPLVSVISTFLLGMGFALLFNDDRIKAKGAWRSLLILPYALPGFVTA
                     LVWRQMFNQDPNFGLINKTFGWSIDWLGDPTWAKVAILITNLWLGFPYMFLICTGALQ
                     SVPGDVKEAAKIDGASGFRTIRSIVMPLVLVAVGPLLVASYAFNFNNFALIYLLTNGG
                     PFEGNQTQVGSTDLLITYAFRLAIGGTTPNFGFAAAISMFIFIIVALISLQGFRSTAS
                     LEDVN"
     gene            complement(3660..4970)
                     /locus_tag="PLK64_09405"
     CDS             complement(3660..4970)
                     /locus_tag="PLK64_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
                     /protein_id="HPV80006.1"
                     /translation="MHMRVYGAAALAGLSALVLSACGGSTGTATSTTAATTAKAAATT
                     AAAPATTGAPVRDAAVDLVIWCDQDRKPIIDKYAAAFGAEQGIKVAVQVSTDVRKDFK
                     DATNAGKGPDVIVGAHDWLGELVQNQAVKPVNLSPTDQAKFPAEAMAAAKFNGQVYGV
                     PYAVENIGIVRNTALAPDAPKTFEEMVTKGTQLVKDGKATNAVLLTVSKTGDAYHSYP
                     FLKAFGGGVFAQKPNGDYDPTKVIVGSPESILGGEQLAKLGKDGILSTNVDGGIVDQV
                     FDAGKAPYAMTGPWAVEKAKKAGISYAISPLPTIAGKQMTPFLGVQMFYVSAKAKNDA
                     FAQEFVTKYVTRKDLQIDMYNIGKRPPALTEAYTEVAAKDADIKAWFEAGKGALPMPN
                     IPAMNSVWGPLGQATADIIAQKATAADRLKAAQTEIEANIVKAG"
     gene            5197..6306
                     /gene="ugpC"
                     /locus_tag="PLK64_09410"
     CDS             5197..6306
                     /gene="ugpC"
                     /locus_tag="PLK64_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009359150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HPV80007.1"
                     /translation="MATVRFKDATRHYPGNPKPTIDKLNIDIADGEFLVFVGPSGCGK
                     STSLRMLAGLEEVNGGQIFIGDRDVTHLSPKDRDVAMVFQNYALYPHMSVADNMGFAL
                     KIAGVSKDEIAKRVLEAAKILDLEPYLERKPKALSGGQRQRVAMGRAIVRSPQVFLMD
                     EPLSNLDAKLRVQTRTQIASLQRRLGVTTVYVTHDQTEAMTMGDRVAVLKDGYLQQCD
                     APRYMYDHPANVFVAGFIGSPAMNLLDVPVVDGGVKLGAATVAIPREILAKASGSRVT
                     LGVRPEDLDLSTDGAGLQVTVDVVEELGADAYVYGTPEGVQQIGTDGDEVGNKPFLAR
                     VDGRRPPKRGDRVHLVPKPGHIHLFDSASGVRLND"
     gene            6467..>7821
                     /locus_tag="PLK64_09415"
     CDS             6467..>7821
                     /locus_tag="PLK64_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HPV80008.1"
                     /translation="MALEITSARPDPALFDLPWSTPLEDWPESHLAALPRGISRHIVR
                     FVKLSGGVLAVKEIKADIAAREFGMLRSLVRMDLPCVTPVGVVTGRIGTDGEPLDACL
                     ITRHLQFSLPYRALYSQALRPDTAARLVDALAVLLVKLHLDGFWWGDVSLSNTLFRRD
                     AGAFAAYLVDAETGEMHARLSDGQRAHDLEIARVNIAGELMDLQAGGFLEDHTDPVET
                     ATEIVRRYESLWSELTGVERFETGDRWRVDERIRRLNALGFDVGELAMSTDIGGTTIQ
                     IQPKVVDAGHHSRRLLRLTGLDVEENQARRLLNDLDSFRAATDRLGDDESVVAHDWLV
                     NVYEPTVRAVPRELRGKLEAAEVFHEVLEHRWYLSERAGRDLPLADTIQDYVRTVLPT
                     KPDESAVLGIDTRAMPVLGGDLDLDGDSDLDEADDDTNGDVGDEPRDDSSDDSRRAKT
                     SP"
BASE COUNT         1298 a         2566 c         2727 g         1230 t
ORIGIN      
        1 gcgacgtcga cccgccaccc atccagcccg ccatcggggc cgagccagcg ctggatcacc
       61 ccgcgcgggt cctcgaacag tcggcgacgc agttccgggc tgctgtggtc gagcttgggg
      121 agcgagggga cgccgagcca cgcggcatac tcctggccgg gtccgaagaa gtagaaggcg
      181 cgctccgggg cggactcgtc cgcgcgcgcg gcctcgaacc actcgtgggt gaccccggtg
      241 tggttgctgg tgaagtctcc gaggacgcgt atgccgcgtg cgtgcgccgc gtcggtgagc
      301 cgccgcagtg ccgcgtcgcc gccaaggagc gggtcgacgg cgtcgaaggt gtgggcgtca
      361 taacggtggt tggagcgccc ggggaagaac ggcgtgaggt agacgacgtc ggcgccgagg
      421 gccgcgatgt ggtcgaggtg ctcggcgacg ccgtcgaggt caccgccgta gagctggcgg
      481 ctgccacccg cccgcgagcg ggtctccacc gggtccgacc aggccgcctc gacggcccag
      541 tcgggggtct cccgctcgtc ggcggcggcc gagcgggcga agcggtcggg gaagacctgg
      601 tagacgacgg cgtccagggg ccactgcggc agcggggtgt ccacggcgac gatccggaag
      661 ttcgcggcgt cggggacatc ccgcaggtgg ctcccggccc cgttgaccca cagccacccg
      721 atcgcgccgc cggagagcag ccagcggtag gacgtcaccg ggttgcgaca atccacctcg
      781 gcgcgccacc acacctcccg ctcggtggag cgttcggcat cgcgcacggc cgtgacgaag
      841 tggggttccc catcacgcag aacccgcacg tatgccgcgt cgaagggcgc ctccccgggg
      901 gccgtcgcga ccgcgggaac ccgcaaccac aggctaacga ccgcaccgag gcgaggcgtc
      961 tcggtcgaga cgtagagcgc cgaaccgtcg tggtggggcc ggttcagacc gatggccggc
     1021 gtgctgatca gcccggtgac cggcccggtg accggcagcg ccggatcgaa ggacgcggtc
     1081 acttcaccga cccggcggtg agaccaccga tgagctggcg ctggaaggag aggtagatga
     1141 ggacgatcgg tgctgaggcg aggaccgccc cgaagcagaa ggccccgaag tacttgttgc
     1201 ggtcggcggt cagcagcgcg ttgaggccga cgccgagggt ctgggcgtcg acatccttga
     1261 ggaagacgct ggccaggagg aactcgttga acaggccgat gaattggatc atcccgacgg
     1321 tgacgagaat cggggtgatg agcggcagag tgatggtgaa gaacacccgg gcatggctgg
     1381 ccccgtcaac cttggcggcc tcgtcgagct ccttcgggac ggtgtcgaag tagcccttga
     1441 gcagccacac gttgccgccc atggcgccgc cgaggtaggc gaggatcagg ccgagcgagg
     1501 tgttgagccc gaactgcggg atgacgtcgc cgagctcggt gaagatccgg aacagcgcga
     1561 cgaaggtgag cagcgacggg aacatctgga tgagcaacag ggtcatcagg ccgggtcgcc
     1621 gaccctggaa ccgcagccgc gagaaggcgt atgccgcgca caccccgatg aacaccgcgg
     1681 cgatcgagcc gatcccggcg atgatgaggg tgttcttgta ccaggaccag aacggccgcg
     1741 aggccgtgag atcggtgtag ttgccgagcc cgaacgcgtc ggggatgagc tgcgtggtgt
     1801 tgaggttgcc ggacgggttg accgcggcgg cgacgatgaa ccaaatcggg aagagcgcga
     1861 agatcagcgc cgccccgatg gccaggtagc gcaccacctg ccaggggtcg cgccgcttgg
     1921 ccgtaggtgg ggcgtcgaag gcatgggtcg gggtggacgg cgccggcggg gtggacggcg
     1981 tggacacggt ggcggtcatc agttcacgtc ctcgagagaa gcggtgctac ggaaaccctg
     2041 cagggagatg agggcgacga tgatgaagat gaacatcgag atcgccgctg cgaagccgaa
     2101 gttgggggtc gtgccgccga tagccaaacg gaacgcatag gtgatgagca ggtcggtcga
     2161 gccgacctgg gtctggttcc cctcgaacgg cccgccgttg gtcagcagat agatgagcgc
     2221 gaagttgttg aagttgaagg cgtaggacgc gacgagcagc ggcccgacag cgacgagcac
     2281 gagcggcatg acgatcgagc ggatcgtccg gaagccggag gcgccgtcga tcttcgcggc
     2341 ctccttgacg tcccccggca ccgactggag agctccggtg cagatgagga acatgtaggg
     2401 gaagcccaac cacaggttgg tgatgaggat ggcgaccttg gcccaggtcg gatcacccag
     2461 ccagtcgatg ctccacccga aggtcttgtt gatcagcccg aagttcgggt cctggttgaa
     2521 catctgacgc cacaccagcg ccgtgacgaa gcccggcaag gcgtaaggga ggatgagcag
     2581 cgagcgccag gcgcccttcg ccttgatccg gtcgtcattg aacagcaggg cgaaccccat
     2641 gcccagcagg aacgtcgaga tgaccgagac gagcgggaag atgaggttcc acacgaggat
     2701 cttgaggaag ccggtgcgta gcgtctcgtt ggtcaaagcc tcggtgtagt tgcgcagccc
     2761 gacgttctcc tgccagcccg acgtcaatga ctgcccttgg ccgtcttccg gcacccagag
     2821 cgcgttctgc ggcagatacc gcttgcccgt cgtggtgtcg atcatccggt cagcggcagc
     2881 gtcgtattgc atcgacgcct tgccgacgaa ggcctccgag agccctgagg tcttgatgcc
     2941 gccgccctcg gtcggcaccg ccagcgcgga caggtccgcc gaacgggcat tcacctcgcg
     3001 ggcgttaagg atcgtgaagc cgttggcctt ggtgaccttg ccggtcgggc tcttctcgac
     3061 cccgtcggtg agcggcgcga ggccctccgg cgtaccggca tacgtcgcgc cgtccttggg
     3121 gtcggtgagc aggagcgtga tggtgcccgt ggtgacgtcc gcgcccgtcg gcaccgcgac
     3181 agacatcgca tagcgcggtg ccccctcgac ctccctgacc gactgggcga tgaggtactt
     3241 gaccgactcc tctttggtcc ccatgtggcc gtcgccgtag ttcgtaaacg acgtcgcgat
     3301 cgtgaagatg atcggccaga tctgcagggc gacgaggaag aagaggcccg ggaagaggta
     3361 cttcagcggg acggctcggg tggtgccgta gatcgcgagc acacacatgg cgatgaacgc
     3421 gacgccgacg acggcccaga ggctgcccgc ggcggcgaac ttgccggcga tccacacgag
     3481 gagggcgagg gcggcggcga tggccaccca tttgacgact atccaggggc tcttggtcat
     3541 ggggactcca gggctgctgc gggggcgagg gcgagggacg agggaaagag cgcaggggta
     3601 tgccgctcgg gccgaccggg tggccggccg gcccgagcgt caggtcaggt cagcaacggt
     3661 cagccggcct tgacgatgtt cgcctcgatc tcggtctgcg ctgccttgag tcggtcggca
     3721 gccgtggcct tctgggcgat gatgtcggcc gtggcctggc cgagcggacc ccagacggag
     3781 ttcatcgcgg ggatgttcgg catcgggagg gcacccttgc cggcctcgaa ccacgccttg
     3841 atgtcagcgt ccttggccgc gacctcggtg taggcctcgg tgagggccgg cgggcgcttg
     3901 ccgatgttgt acatgtcgat ctgcaggtcc ttgcgcgtca cgtacttggt gacgaactcc
     3961 tgcgcgaagg cgtcgttctt ggccttggcc gagacgtaga acatctggac gccgaggaac
     4021 ggggtcatct gcttgccggc gatcgtcggc agcggcgaga tggcgtagga gatgccggcc
     4081 ttcttcgcct tctcgacggc ccacggaccg gtcatggcgt agggcgcctt gcccgcgtcg
     4141 aagacctggt cgacgatgcc gccgtcgacg ttggtcgaga ggatgccgtc cttgccaagc
     4201 ttggccagct gctcgccgcc gaggatcgac tcgggcgagc cgacgatgac cttggtcggg
     4261 tcgtagtcgc cattgggctt ctgggcgaag acgccgccgc cgaacgcctt gaggaacggg
     4321 taagagtggt aggcgtcacc ggtcttgctc acggtgagca gcacggcgtt ggtcgccttg
     4381 ccgtccttga ccaactgggt gcccttggtg accatctctt cgaaggtttt gggcgcgtcg
     4441 ggcgcgaggg ccgtgttgcg gacgatgccg atgttttcga cggcataggg gacgccgtag
     4501 acctggccgt tgaacttcgc ggcggccatg gcctcggccg ggaacttggc ctggtcggtc
     4561 ggcgagaggt tgaccggctt gacggcctgg ttctggacca gctcaccgag ccagtcgtgg
     4621 gcgccgacga tgacgtccgg gcccttgccc gcgttggtgg cgtccttgaa gtccttgcgg
     4681 acgtcggtgg acacctggac ggcgaccttg atgccctgct cggcgccgaa ggccgcggcg
     4741 tacttgtcga tgatgggctt gcggtcctgg tcgcaccaga tgacgaggtc aacggcggcg
     4801 tcgcggacgg gggcccccgt cgttgcgggg gcggcggcgg tcgtggccgc tgccttggcg
     4861 gtcgtagccg cggtcgtgct cgtagcggtc ccagtggacc cgccacaggc gctgaggacc
     4921 agggcggaca agccggcgag ggctgccgct ccatagacgc gcatgtgcat ggactctcct
     4981 tcgaagccgg acggcccggc ggccgtcctc accccaacga cactaggagt ctctgaaaga
     5041 aattacaagg gtcctatgaa agatgtttca cggcctcact ggacagtgac ctcatccatg
     5101 cgcaaagatg acagcggttc caccgtccgg tggcgcacat caccctttac aacggatcgt
     5161 ccggcacgtt cctgccggtg aaggagtcgt ataaccatgg caactgtccg cttcaaggac
     5221 gccacccggc actacccggg caaccccaag ccgacgatcg acaagctgaa catcgacatc
     5281 gcagacgggg agttcctcgt cttcgtcggc ccctccggat gtggcaagtc cacctccctg
     5341 cgcatgctcg ccggcctcga agaggtcaac ggcggccaga tcttcatcgg tgaccgcgac
     5401 gtcacccacc tgtcccccaa ggaccgcgac gtcgccatgg tcttccagaa ctacgcgctc
     5461 tacccgcaca tgtcggtcgc cgacaacatg ggcttcgccc tgaagatcgc tggcgtctcc
     5521 aaggacgaga tcgccaagcg cgtcctcgag gccgccaaga tcctcgacct ggagccctac
     5581 cttgagcgca agccgaaggc cctctccggt ggccagcgtc agcgtgttgc catgggccgc
     5641 gccatcgtcc gctcccccca ggtcttcctc atggacgagc cgctgtccaa cctcgacgcc
     5701 aagctgcgcg tgcagacccg cacccagatc gcgtcgctgc agcgccgcct cggcgtcacc
     5761 acggtctacg tcacccacga ccagaccgag gccatgacca tgggtgaccg cgttgcggtc
     5821 ctcaaggacg gttacctcca gcagtgcgac gccccgcgct acatgtacga ccacccggcc
     5881 aacgtcttcg tcgccggctt catcggctcg cccgcgatga acctgctcga cgtcccggtc
     5941 gttgacggcg gagtcaagct cggcgccgcc acggtcgcca tcccgcgcga gatcctggcc
     6001 aaggccagcg gcagccgggt caccctgggt gtccgccccg aagacctcga cctgtccacc
     6061 gacggagccg gcctgcaggt cacggtcgac gtcgtcgaag agctcggcgc cgacgcctac
     6121 gtctacggca cccccgaggg tgtgcagcag atcggcaccg acggtgacga ggtcggcaac
     6181 aagccgttcc tcgcccgcgt tgacggccgt cgtcccccca agcgtggcga cagggtgcac
     6241 ctggtcccga agccgggcca catccacctg ttcgactcag ccagcggggt tcgcctgaac
     6301 gactgagcgc cgccgccggt ccccgcctgc gcggaccggc atacgccaat gacggtatgc
     6361 cgcggccggc gccttcgatc cgaaggtgcc ggccgcgtgc tgtcccgtca acggaccacc
     6421 gacctgcttc gtccggcagc acccatctgg aagcgaatcg ctcactgtgg cactggaaat
     6481 cacctctgcc cgtcccgacc cggcgctgtt cgacctgccg tggtcgacgc ctctggagga
     6541 ctggccggaa agccacctcg ccgccctccc ccgcggtatc tcgcggcaca tcgtccgctt
     6601 cgtcaaactg tccggcggag tcctggccgt caaggagatc aaggctgaca tcgccgcccg
     6661 ggagttcggc atgttgcgct ccctcgtgcg gatggacctg ccgtgcgtca ctccggtcgg
     6721 cgtcgtcact gggcggatcg gcaccgatgg cgagccgttg gacgcgtgcc tcatcactcg
     6781 gcacctgcag ttctcgctgc cctatcgcgc gctctattcg caggcactgc gacccgacac
     6841 ggccgcccgc ctcgtcgacg ccctggccgt cctgctcgtc aaactgcacc tcgacgggtt
     6901 ctggtggggc gacgtctcgc tctccaacac gttgttccgc cgcgatgccg gcgcgttcgc
     6961 ggcatacctc gtggacgccg agaccggcga gatgcacgcg cgactgtcgg acggtcagcg
     7021 ggcccacgac ctggagatcg cgcgggtcaa catcgccggt gagctgatgg acctgcaggc
     7081 cggcggcttc ctcgaggacc acaccgaccc ggtcgagacg gcgaccgaga tcgtccgtcg
     7141 ttacgagtcg ctgtggtccg agctcaccgg ggtcgagcgg ttcgagaccg gcgaccgttg
     7201 gcgcgtcgac gagcggatcc ggcggctcaa cgcgctcggc ttcgacgtgg gtgagctcgc
     7261 gatgtcgacc gacatcggcg gcacgacgat ccagatccag ccgaaggtcg tcgacgccgg
     7321 ccaccacagc cgtcggctgc tgcgtctcac cggcctggac gtcgaagaga accaggctcg
     7381 acggctgctc aacgacctcg actcgttccg ggcggccacc gaccgcctgg gtgacgacga
     7441 gtccgtggtc gcccacgact ggttggtcaa cgtctacgag ccgaccgtgc gggcggtgcc
     7501 gcgcgagttg cgcggcaagt tggaggccgc ggaggtgttc cacgaggtgt tggagcaccg
     7561 gtggtatttg tccgagcgcg ccgggcgcga cctccccctt gccgacacga tccaggacta
     7621 cgtgcggacc gtgctgccga ccaagcccga cgaaagtgcc gtgctgggca tcgacacccg
     7681 cgccatgccg gtcctcggcg gcgacctcga tctcgacggc gattccgacc tcgatgaagc
     7741 ggatgacgac acgaacggcg acgttggcga tgagccccgc gatgactcca gcgatgactc
     7801 ccgcagggcg aagacctccc c
//
LOCUS       DAOMZY010000148         7815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_8790_length_7815_cov_6.146521, whole genome shotgun sequence.
ACCESSION   DAOMZY010000148 DAOMZY010000000
VERSION     DAOMZY010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7815
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8790_length_7815_cov_6.146521"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..1152)
                     /locus_tag="PLK64_09420"
     CDS             complement(16..1152)
                     /locus_tag="PLK64_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2183 domain-containing protein"
                     /protein_id="HPV80009.1"
                     /translation="MARPHASAVVEDAWNSQRERVLRKRGWGTRVISHTGYGSTDFVR
                     VFARVVLGRALGDDDEPRQDATYGGMRSPYLRRRGWRAFFTAPAMNVPVEVHVGHHVA
                     HGRSDRSGHIDLTFRGHGMPPGWHEVTVSAQGCDPVQASVYIVAPDARFGLISDIDDT
                     VITTSLPRPMIAAWNTFFRHENARRVVPGMGPLYRTIMAEHPGAPIIFVSTGAWNTAP
                     TLTRFLKRNGFPIGPLLLTDWGPTNTGWFRSGQDHKRACLDRLAKDFPKIKWLLIGDD
                     GQHDPKIYQDFLEARPKRVAAVGIRQLTPAEQVLSHGLPVSTEELTGKAPRPRTVAPI
                     VYAPDGYGLLRLLRAAGLLAPSPAGEAGLGAADGSATSDPLDSH"
     gene            1233..1628
                     /locus_tag="PLK64_09425"
     CDS             1233..1628
                     /locus_tag="PLK64_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012802102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPV80010.1"
                     /translation="MSGWTGNIERATLDNDTFRTVVATGAHLQLVVMSLAPGEEIGLE
                     VHHDRDQFLRVEEGAAMVTLGPDDEVIEKTIALGEDWACIIPAGRWHNVINCGHGPLK
                     LYSIYSPPEHPDGTVHATKADADADEHEL"
     gene            complement(1678..1809)
                     /locus_tag="PLK64_09430"
     CDS             complement(1678..1809)
                     /locus_tag="PLK64_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80011.1"
                     /translation="MDLILALATVIGALIVPMEITHRRTRSLPRLTQLERCGIRQWW"
     gene            1904..2920
                     /locus_tag="PLK64_09435"
     CDS             1904..2920
                     /locus_tag="PLK64_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV80012.1"
                     /translation="MPLRTVAVLLTERPLAFEFGVLAEVFGIDRTADGVPPFEFRVCA
                     ERPGEPLPMDNGVSLTVPFGLDGLRGADVVAVSSTQAKTFSEPVLEAVRQAHAAGATI
                     LATCSGGFVLAEAGLLDGRTATCHWYHAAEFAERYPATTVDATVLFVEDGTILTSAGT
                     AASIDACLHLVRREFGADVATRIARRMVVPPQRDGGQRQFVEQPIPTIAAESLEPLLT
                     WMMEHLEEDLPVRELARHAHLSERTFARRFLAETGTTPAKWIASQRLHHACRLLERTD
                     LPIEQVALRSGFGTAALLRDHFARNVGVPPADYRRTFRCSDDTATIPDTATIPDTADT
                     PEAS"
     gene            2924..4087
                     /locus_tag="PLK64_09440"
     CDS             2924..4087
                     /locus_tag="PLK64_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPV80013.1"
                     /translation="MPLDLHALTPDQLRARRTLKWTAYPADLLPMWVAEMDFPVCREV
                     TAALTRAVAAESFGYPSPVVHAELADALVEWQAQQHGWTVDPAGVHSVGDVMHGVDLA
                     IRYFSGPDDPVIIPTPVYMPFFDIVRLTGRPQVHVPMSWDGARWTFDLAAIDAAFAAG
                     GRTVLISSPHNPLGRVFDAAELAGLAAVVARHGGRVISDEIHAPLVFDRPHLPYAAAS
                     PEAADHSITVLSASKAWNLPGLKCAQVITTNAADTAAWKAMPYWETVGVSTLGMEASI
                     AAYREGGEWLAQVVATLAEHRDLVAQAVASWDGVTTVPNEGTYLQWLDFTRLGLDVEP
                     SAWLVEQARVLVNPGVPFGGEPRRFARLNFATTRSLLDEGLGRIEQAVRSRRP"
     gene            4169..>5745
                     /locus_tag="PLK64_09445"
     CDS             4169..>5745
                     /locus_tag="PLK64_09445"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPV80014.1"
                     /translation="MRVPFFRPAAALAVGVLVVVGSLAACTSAPEPPTPEPVAAALAA
                     GLTSGDLNAVVFDGATPAAATDSVRNAVGALSSITRTVTVESVTRAADDANSASAKLR
                     WTWNLTGADGAWAYSVDAKLARAADQSWHVAWSPTLVEPKLTATERLALERRQAARGS
                     ILGANGEVLVGLKDVYRVGIDKGRISADQAQISATALATLMGTDATAYAKTVAASGPA
                     QFVPAITLRIDDPRIAGKTAAVQAIPGGAAMPGQAMLAPTATFARALLGTVGPATAEL
                     VSKSGGRIVAGDTSGLSGLQLRYDLPLSGQPGVVVLAKGTDSGGAASSRTLFTKAPVP
                     GVSLTTTLDRTAQDAAEQVLTGAPGVASLVAIRPSTGEIVAVANSPATNGQAVATTGQ
                     AAPGSTFKIVTSLALLRAGLTPESPMSCTDTVTVDGRVFKNYDAYPASKLGSLPLRLA
                     LANSCNTAFISQRGTVSQDDLADAAAALGIGVAYDTGYSGFVGSIPRDATQTEHGASM
                     IGQGRVTASALTMAIVMA"
     assembly_gap    5746..5845
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5846..6201
                     /locus_tag="PLK64_09450"
     CDS             <5846..6201
                     /locus_tag="PLK64_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019134900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPV80015.1"
                     /translation="ATAAATAAATDSPAPKISPAPTAPPAPAKPLTPEEAQALQGMLR
                     GVVAEGSAKALAGIAEGAKTGTAEFDAAGVTKAHAWMIAFRGDLAVAVYVADGVSGSQ
                     TAGPLVSAFLQAYAG"
     gene            6802..7218
                     /locus_tag="PLK64_09455"
     CDS             6802..7218
                     /locus_tag="PLK64_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HPV80016.1"
                     /translation="MSDTQQAEVVDEGSIAPDTIEVSALLSHDLNHVWHVLSTNDGVA
                     AVLGDGATLGNKGEHWRATDGTHGVWRSYHPLEQVRLSWHASEDGPRSLVDLHLAPQG
                     EQTYVSIRHEHVEGDLDSLKARWAAALSRLEAAAAG"
     gene            7334..>7815
                     /locus_tag="PLK64_09460"
     CDS             7334..>7815
                     /locus_tag="PLK64_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HPV80017.1"
                     /translation="MTAAPASPEPSLQEPPPHAGLQRWLPILTWLPTYERRWIKDDLL
                     AGLGLTALLVPQGMAYAQLAGLPPITGLYTSIMCLVAYAVFGPSRVLVLGPDSSLGPM
                     IAASILPLVAANGDPMRAVVLASAQSVLVGIFMIAAGAAKLGFVADLLSKPTMVGYMN
                     G"
BASE COUNT         1162 a         2854 c         2563 g         1136 t
ORIGIN      
        1 ggcgacctcc tggctctagt gggagtcgag tgggtcggac gtggccgagc cgtctgcagc
       61 cccgaggccc gcctcaccgg caggggacgg cgcgagcaga cccgcggcgc gcagcaagcg
      121 caacagcccg taaccgtccg gcgcatagac gatcggcgcc accgtgcgcg gccgcggcgc
      181 cttgccggtg agttcctcgg tcgacaccgg caaaccgtgc gagagcacct gctcggccgg
      241 cgtcaactga cggatcccga cggcagccac ccgcttgggc cgcgcctcga ggaagtcctg
      301 atagatcttc ggatcgtgct ggccgtcgtc accgatgagc agccacttga tcttggggaa
      361 gtccttggcc agtcggtcca ggcacgcccg cttgtggtcc tgaccgcttc ggaaccagcc
      421 cgtgttcgtc ggcccccagt cggtgagcag cagcggcccg atggggaaac cgttgcgctt
      481 gaggaagcgg gtcagcgtcg gcgcggtgtt ccacgccccc gtcgacacga agatgatcgg
      541 cgcgcccgga tgctcggcca tgatcgtccg atagagcggc cccatccccg gcaccacccg
      601 gcgggcgttc tcgtgccgga agaaggtgtt ccacgcggca atcatcggcc ggggcagtga
      661 cgtcgtgatg accgtgtcgt cgatatcgct gatcagcccg aaccgcgcgt ccggcgccac
      721 gatgtagacg ctcgcctgca cggggtcgca cccctgggcg ctgacggtca cctcgtgcca
      781 gccgggcggc ataccgtgtc cgcggaaggt gaggtcgatg tgaccgctgc ggtcactgcg
      841 gccgtgagcc acgtggtggc cgacgtgcac ctcgaccggc acgttcatcg cgggggcggt
      901 gaagaaggcg cgccagccac gccggcgcag gtagggcgag cgcatcccgc cgtaggtcgc
      961 gtcctgacgc ggctcgtcgt cgtcgcccag cgcgcggccg agcacgaccc gggcgaacac
     1021 ccggacgaag tccgtcgagc cgtagccggt atggctgatg acccgggttc cccacccgcg
     1081 tttgcgtagg acgcgctccc gttgggagtt ccaagcgtcc tcgactaccg ccgaggcgtg
     1141 cgggcgtgcc atgggggaag gctatggcat ccggtatgcc gcggcgccgc cccccggggt
     1201 tatcgtcgag tcagcagacg aaggagaggc ccatgagtgg ctggactggg aacatcgagc
     1261 gcgcaacgct cgacaacgac accttccgga cggtggtggc gaccggcgct cacctgcaat
     1321 tggtcgtcat gagcctcgcg cccggcgagg agatcgggct cgaggtgcac cacgaccgcg
     1381 accaattcct gcgcgtcgag gagggtgccg cgatggtgac gctcgggccg gacgacgagg
     1441 tcatcgagaa gaccattgcg ctgggcgagg attgggcctg catcatcccc gccggccggt
     1501 ggcacaacgt catcaactgc ggtcatggcc cgctcaagct gtattcgatc tattcgccgc
     1561 cggagcaccc ggacggcacg gtccacgcga ccaaggccga cgcagacgcc gacgagcacg
     1621 agctgtaacg cggcggccga cgcgcgggct ggcacgccgg ccgacacgcg ggtcggctca
     1681 ccaccactgg cggatgccgc accgctccag ttgggtcagg cggggcaaac tccgcgtgcg
     1741 acggtgggtg atttccatgg ggacgatgag cgcgccgatc acggtcgcga gtgccaagat
     1801 gaggtccatg gcagtaatca tcccctcatc tgattactgc cgatagtggc aggacagtcc
     1861 ctccccgtgg cttttctgcc aaacccgcgg catactcagg ggtatgccgt tgcgcaccgt
     1921 tgccgtgctg ctcaccgaac gcccgctcgc gttcgaattc ggcgtcctcg cggaggtttt
     1981 cggcatcgac cggaccgccg acggggtgcc gccgttcgag ttccgggtct gcgccgagcg
     2041 gccaggtgag cccctcccca tggacaacgg ggtgagcctc accgtcccct tcggcctcga
     2101 cgggctgcgc ggcgccgatg ttgtcgccgt ctcctcgacc caggccaaga ccttctccga
     2161 gccggtgctt gaggccgttc gccaggccca cgccgccggc gccacgatcc tggccacgtg
     2221 ttcgggcggc ttcgtcctcg ccgaggcggg cctgctcgac ggccggacgg cgacctgcca
     2281 ctggtaccac gctgccgaat tcgccgagcg ctatcccgca accaccgtcg acgcgaccgt
     2341 cctcttcgtc gaggacggca ccatcctcac cagcgctggc accgcggcga gcatcgacgc
     2401 ctgcctgcac ctggttcgcc gcgagttcgg cgccgacgtc gcgacccgga tcgcccggcg
     2461 catggtcgtt cccccgcagc gcgacggcgg tcagcggcag ttcgtcgagc agccgatccc
     2521 cacgatcgcc gctgaaagcc tcgagccgct gctcacctgg atgatggagc acctcgagga
     2581 ggacctgccg gtccgtgagc tggcccggca cgcgcacctg tccgaacgca ccttcgcacg
     2641 tcggttcctc gccgagaccg ggacgacgcc cgcgaaatgg attgccagcc aacgacttca
     2701 ccacgcctgc cggctgctcg agcggaccga tctgccgatc gagcaggtcg ccctgcgcag
     2761 cgggttcggc acggcggcgc tgctgcgcga ccacttcgcg cgcaacgtcg gcgtcccccc
     2821 ggccgactac cggcgcacct tccgctgctc cgacgacacc gcgaccatcc ccgacactgc
     2881 gaccatcccc gacactgccg acacccccga agcgagttga cccgtgccgc tggacctgca
     2941 tgccctcacc cccgaccagt tgcgggcgcg gcggaccctc aagtggacgg cataccccgc
     3001 ggatctgttg cccatgtggg tggccgagat ggacttcccc gtatgccgtg aggtcaccgc
     3061 cgcgctcacc cgcgccgtcg cggccgagag cttcggctac ccctcgccgg tggtccatgc
     3121 cgagttggcc gacgcgctcg tcgagtggca ggcgcagcaa cacgggtgga ccgtcgatcc
     3181 ggccggggtc cacagcgtcg gcgacgtcat gcacggcgtc gacctggcga tccgctactt
     3241 cagcgggccg gacgacccgg tgatcatccc gacgcccgtc tacatgccgt tcttcgacat
     3301 cgtgcgcctc accggccgcc cgcaggtcca cgtcccgatg tcctgggacg gcgcccggtg
     3361 gaccttcgac ctggccgcca tcgacgcggc gttcgccgcc ggtggtcgca ccgtcctcat
     3421 ctcgtcaccg cacaacccgc tcggtcgggt cttcgacgcc gccgagcttg ccgggctcgc
     3481 ggccgtcgtc gcgcggcatg gcggtcgggt catctccgac gagatccacg ctccgttggt
     3541 cttcgaccgc ccacatctcc cgtatgccgc cgcctcaccc gaggcggccg accacagcat
     3601 cacggtcctc tcggcatcga aggcgtggaa cctgccgggc ctcaaatgcg cccaggtcat
     3661 cacgacgaac gccgccgaca ccgccgcgtg gaaggcaatg ccctactggg agacggtcgg
     3721 ggtctcgacc ttggggatgg aggcgagcat cgcggcatac cgagagggcg gcgagtggct
     3781 cgcccaggtg gtggcaacgc tggccgagca ccgcgatctg gtcgcgcagg cggtggccag
     3841 ctgggacggg gtgacgaccg tgcccaacga ggggacctat ctgcagtggc tcgacttcac
     3901 caggctgggg ctcgacgtcg agccgtcggc gtggctggtc gagcaggccc gcgtgctggt
     3961 caacccgggg gtgccgttcg gcggggagcc ccggcgcttt gcccggctca acttcgcaac
     4021 gactcggtcg ctgctcgacg aggggctcgg ccggatcgag caggcggtgc gcagccggcg
     4081 cccctgatcc gggagtcagg cggcaacgcc ggtggtccgt gggagcggtg cgcggccgtc
     4141 gcccctgatc cgggagagga tgtctgccat gcgcgtcccg ttctttcgac ctgcagcagc
     4201 cctcgctgtc ggcgtgctcg tcgtggtcgg ctccttggcg gcctgcacca gtgctcccga
     4261 gccccccaca cccgagcccg tcgcggccgc cctggcggct ggcctgacct cgggagacct
     4321 gaacgccgtc gtcttcgacg gcgccacgcc cgcagcagcc accgactccg tgcgcaacgc
     4381 cgtcggcgcg ctgtcgagca tcacccggac cgtgaccgtc gagagcgtca cccgagcggc
     4441 ggacgacgcc aactcggcct ccgccaagct gcgctggacc tggaacctca ccggcgccga
     4501 cggtgcctgg gcctactccg tcgacgccaa gctggcccgc gccgccgacc agagctggca
     4561 cgtcgcctgg tcacccacgc tcgtcgaacc caagctcacc gccaccgaac gtctcgccct
     4621 tgagcgtcgc caagccgctc gcggatcgat cctcggcgcc aacggcgagg tcctcgtcgg
     4681 gctcaaggac gtctatcggg tcggcatcga caagggtcgg atcagtgccg accaggcgca
     4741 gatctcagcc accgctttgg cgacgctcat ggggaccgac gccacggcat acgcgaagac
     4801 cgtggccgcc tcgggaccgg cgcagttcgt gcccgccatc accctgcgta tcgacgaccc
     4861 gcggattgcg ggcaaaaccg cggcggtcca agcgatcccg ggcggcgcgg cgatgcctgg
     4921 ccaggcgatg ctcgcgccaa cggcgacctt cgcacgggcg ctgctgggga ccgtcgggcc
     4981 cgcgacggcc gagttggtca gcaagtccgg cgggcgcatc gtcgccggcg acacctcggg
     5041 cctgtccggg ctgcagttgc gctacgacct gccgctgtcg ggccaaccgg gcgtggtcgt
     5101 cctcgccaag gggaccgaca gcggcggtgc ggccagctcg cggaccttgt tcacgaaggc
     5161 tcccgtcccc ggtgtctcgc tgacgaccac cctcgaccgc acggctcagg acgcggccga
     5221 acaggtgctc accggcgcac ccggggtggc aagcttggtc gccatccggc cctcgaccgg
     5281 cgagattgtc gccgtcgcga actcaccggc caccaatggg caggccgtcg ccacgacggg
     5341 ccaggcggcg cccggatcga cgttcaagat cgtcacgtcc ctcgccctgc tgcgcgccgg
     5401 cctgacgcct gagtcgccga tgtcctgcac cgacacggtg accgtcgacg ggcgggtctt
     5461 caagaactac gacgcctatc ccgcctcgaa actcgggtcg ctgccgttgc ggctggcgct
     5521 ggccaactcg tgcaacaccg ccttcatctc gcagcgaggg accgtctcgc aggacgacct
     5581 cgccgacgcg gcagccgcct tggggattgg ggtcgcctac gacaccggat acagcggctt
     5641 cgtcggatcg atcccccgcg acgcgacgca gaccgagcat ggcgcctcga tgatcggtca
     5701 gggccgcgtc accgcctcgg ccctgacgat ggcgatcgtc atggcnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnncggcg accgctgcag cgactgctgc agcgacggat
     5881 agtcccgcac cgaagatcag tcctgcgccg accgcacccc ctgccccggc caagcccctc
     5941 acgcccgagg aagcccaagc gctccaaggc atgctgcgcg gcgtggtcgc cgagggcagc
     6001 gccaaggcac tcgccggcat tgccgagggg gccaagacgg ggaccgccga gttcgatgcc
     6061 gccggggtca ccaaggcgca cgcctggatg atcgccttcc gcggtgacct cgcggttgcc
     6121 gtctatgtgg cggacggcgt ctccggctcc caaaccgccg gcccgttggt gagcgccttc
     6181 ctccaggcgt atgccgggta actcaccccc agccgcccct ccccgccaga cccgccagac
     6241 ccgccagacc cgcttgaccc gcttgacccg cttgatccgc ttgatccgcc agcatctccc
     6301 cgccagatcc gcttgcccac gcctggtccg agcccgccgc acctccccgc cccctgccag
     6361 atccgccatt cacgcctggc tcgagcccgg ccccaccgct tgtatgaggc tagccacgct
     6421 tgtatgccgg tagagcctca tacaagcgtc actaacctga tacaagccgc gggggcatca
     6481 ccggccaccc agaaatcccc ggcgagtgtg acgaaatcgg gaccacccgg catacccctc
     6541 gcccagatca ccggcgagtg tcaggaactt gggacaccca gcgccccgcg acccccagat
     6601 caccggcgag tgtgacgaaa tcgggaccac ccggcatacc cctcgcccac aacaccggcg
     6661 agtgtgaggg gatcggggga gcgagtcgga tcttgggggc aaacccgagg ttgcgttcct
     6721 gcggcgtttg gagtcggatg gacccagcag gaaaccgcgg ccccgccgca ggcgagcggc
     6781 cgctcgagaa ggaggcaaat gatgtccgac acgcagcagg ccgaggtggt cgacgaagga
     6841 tcgatcgcgc ccgacaccat cgaggtgagt gcgctgttgt cccacgacct gaaccacgtg
     6901 tggcacgtgc tgagcaccaa cgatggcgtg gcagcggtcc tcggagacgg cgccaccctg
     6961 ggcaacaagg gtgagcactg gcgagcgacc gacggcacgc atggtgtctg gcgcagctac
     7021 caccccttgg agcaggtgcg gctgtcctgg cacgcctccg aggacggccc gcgttcgctc
     7081 gtcgacctgc acctggcgcc ccagggcgag cagacctacg tgtcgattcg ccacgagcac
     7141 gtcgagggcg acctcgactc gctcaaggcc cgctgggcag cggcgctgag ccggctggaa
     7201 gccgccgcgg caggctaagc agtcccggcg cgatgcgtcg cggggccggg tcaacgggcc
     7261 acgcgacgcg tcgcgaccgt gtgtcgagag cgtttcccgt gacgctgagg cgcaatcgcg
     7321 gcacactcgc ctggtgaccg ccgcaccagc ctcgcctgag ccatcgctcc aggaaccgcc
     7381 gccccacgcg gggctccaac ggtggcttcc gatcctcacc tggctgccga cctacgagcg
     7441 ccgctggatc aaggacgacc tcctggcggg gttggggctc accgccctcc tcgtgccgca
     7501 aggcatggcc tacgcgcagc tcgcgggcct gccgccgatc accggcctct acacgtcgat
     7561 catgtgcctc gttgcgtatg ccgtgttcgg cccctcccgc gtcctcgtcc tcggcccgga
     7621 ctcctccctc ggcccgatga ttgccgcgtc gatcctcccc ctggtcgcgg ccaacggtga
     7681 ccccatgcgg gccgtcgtcc tcgcctcggc ccagtcggtc ctcgtcggca tcttcatgat
     7741 cgcggccggt gccgccaagc tcgggttcgt cgcggacctg ctgtccaaac cgacgatggt
     7801 cggctacatg aacgg
//
LOCUS       DAOMZY010000149         7786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_8832_length_7786_cov_5.443280, whole genome shotgun sequence.
ACCESSION   DAOMZY010000149 DAOMZY010000000
VERSION     DAOMZY010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7786
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8832_length_7786_cov_5.443280"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="PLK64_09465"
     CDS             complement(<1..917)
                     /locus_tag="PLK64_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80018.1"
                     /translation="MTSRLARSALASISLLLAGCTAMSSSPTSTSATDPASGSPTASA
                     TAAADLGSQLAQQILRDYNVRNNPAIVASAAGDPTGWKAADTGITLEQDLFGTVDARV
                     NKTLKPASPFDFTPKELIVASPEGAYPRWAVIEFGESERAGGTASPSATASASPADRR
                     VVGVFVQPVADAPWLMENHITVPRPATSVTAREASAAESADARAALQRALDYLTFGRE
                     APEVDLGSIPGSRSSLLIPAKDNIRDTTLVCSTYVPPAGVPGYGNSRAFAVEDTVVLI
                     GSISCSASVMLKRSTTTNAWQRAILGAAET"
     gene            complement(980..1195)
                     /locus_tag="PLK64_09470"
     CDS             complement(980..1195)
                     /locus_tag="PLK64_09470"
                     /inference="COORDINATES: protein motif:HMM:NF039372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6191 domain-containing protein"
                     /protein_id="HPV80019.1"
                     /translation="MIFGREGASAGGAVFGEAIALLQPGYAHTLAEKRRQESSREVPE
                     DGAPPLIDLEAGVIRITRPPAAEPPAP"
     gene            complement(1302..3545)
                     /locus_tag="PLK64_09475"
     CDS             complement(1302..3545)
                     /locus_tag="PLK64_09475"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPV80020.1"
                     /translation="MEGPEIIFAEATIDNGKFGTRTIRFETGRLAKQADGAVVAYLDD
                     DTMIMATTAAGRTPKDQFDFFPLTVDVEERMYAIGKIPGSFFRREGRPSTEAILTCRL
                     IDRPLRPSFVKGLRNEVQIIITVYALNPDHQYDVVAINAASAATQISGLPFSGPIGGV
                     RVSCIDGHWVAFPNFSDAERSTFDMVVAGRIVGDDVAIMMVEAESTEATWDLVKHQGK
                     TAPTEEVVAEGLEAAKKFIKVLCEAQADLAARSAKETIEFPRFLDYEADVYAAVEGDA
                     HDATAAALTIAGKTEREDTLDAIKSGLHAKFVGDGETVGEFAGRQKEVSAAFRALQKK
                     LVRQRILRDSVRIDGRGLRDIRALGAEVEVLPRVHGSAIFERGETQIMGVTTLNMLKM
                     EQQLDTLSPVTRKRYMHNYNFPPFSTGETGRVGSPKRREVGHGALAERALMPVLPTRE
                     EFPYAIRQVSEALGSNGSTSMGSVCASTLSLLNAGVPLRAPVAGIAMGLVSDTVDGET
                     RYAALTDILGAEDAFGDMDFKVAGTREFVTAIQLDTKLDGIPASVLASALTQAREARL
                     HILDVMGEAIDAPDEMSPFAPRIITVKVPVDKIGEVIGPKGKMINQIQDDTGADISIE
                     DDGTVYIGAVDGPSAEAARAAINAIANPQMPEVGERFMGTVVKTTTFGAFISLLPGKD
                     GLLHISEVRKLVGGKRIDNVDDVLKIGQKVQVELKEIDPRGKLSLAAVMADGEGDKAD
                     AEAPAEG"
     gene            complement(3682..3951)
                     /gene="rpsO"
                     /locus_tag="PLK64_09480"
     CDS             complement(3682..3951)
                     /gene="rpsO"
                     /locus_tag="PLK64_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPV80021.1"
                     /translation="MPLESTVKKQIMTEYATVEGDTGSPEVQIAMLTKRIQDLTEHAR
                     THPHDHHSRRGLLLLVGRRKRMLRYLEATDIERYRSLIKRLGLRR"
     gene            4097..4972
                     /locus_tag="PLK64_09485"
     CDS             4097..4972
                     /locus_tag="PLK64_09485"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HPV80022.1"
                     /translation="MHDIQWIDLEGVVNMRDVGGMPTLDGGTIASGRLIRSDNLQELS
                     DAAIRRLVDELGVTDVVDLRTHVEVAQEGAGPLVGHPSVQISHFTLYADDSAESGIPD
                     SERELPWETQARREAAARARREQANGDVRGTSGDQSSKPHIVPGGQDHDDFWSDHYLS
                     YLGQRPESVVGALRAIADAKGAVVVHCAAGKDRTGTITGLALVVAGARPEAVIADYAA
                     SAERVPQILDRLLRRPAYAANLLGKTVAEQSPRPETMTRLLAALERDFGGVLGWLSTQ
                     DWTEADTEAVRAKLR"
     gene            complement(5130..6974)
                     /gene="lepA"
                     /locus_tag="PLK64_09490"
     CDS             complement(5130..6974)
                     /gene="lepA"
                     /locus_tag="PLK64_09490"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPV80023.1"
                     /translation="MARTGLPPHGTPPDRIRNFCIIAHIDHGKSTLADRMLQLTGVVD
                     SRAMRAQYLDRMDIERERGITIKSQAVRMPWESDGVTYALNMIDTPGHVDFTYEVSRS
                     LAACEGAVLLVDAAQGIEAQTLANLYLAMENDLAIIPVLNKIDLPAAQPEKYAAELAG
                     LIGCDPSDCLQVSGKTGVGVEELLDIIVKQVPHPVGDPNAPARAMIFDSVYDTYRGVV
                     TYVRVIDGDLNPRERILMMSTKATHELLEIGVSSPEPEPSKGLGVGEVGYLITGVKDV
                     RQSRVGDTVTNAGKPARESLGGYRDPKPMVFSGLYPIDGSDYPDLREALDKLKLNDAA
                     LAYEPETSAALGFGFRCGFLGLLHLEIVRERLEREFGLDLISTQPNVVYEVTLDDRRV
                     VTVTNPSEYPDGKISEVREPVVRATILAPSEFIGAIMELCQGKRGALRGMDYLSEDRV
                     EMRYSLPLAEIVFDFFDQLKSRTRGYASLDYELDGDQVADLVKVDILLQGEQVDAFSA
                     IVHKDKAYAYGVEMAGKLRKLIPRQQFEVPIQAAIGARIIARETIRAMRKDVLAKCYG
                     GDITRKRKLLEKQKEGKKRMKMVGRVEVPQEAFIAALSSEPIDPKKKP"
     gene            7091..>7786
                     /locus_tag="PLK64_09495"
     CDS             7091..>7786
                     /locus_tag="PLK64_09495"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HPV80024.1"
                     /translation="MDILVTGGTGTLGRATVPLLQAAGHHVRVLSRRTGPGLTTGDLE
                     RRQGLKRALDGVHTVVHLATSGGARDIRAAANLSKAAVEADVEHIIYMSIVGADRVPL
                     PYYQAKVEAERQLQASGLGLTIQRATQFHELVLTVMRTQRAAPLLFVPDIPIQPVDLS
                     AVAARLTSLIGQETAGRAPDLGGPRVERFTDLAQQWQHHIDRKRRVVPVRLPGRTFAA
                     YAQGGHLAPEGAAT"
BASE COUNT         1205 a         2603 c         2673 g         1305 t
ORIGIN      
        1 gtctcggcgg cgccgagaat ggcccgctgc caggcgttgg tggtcgtgct ccgcttgagc
       61 atcacgcttg ccgagcagga gatcgagccg atcagaacca cggtgtcctc gacagcgaag
      121 gcccggctgt tgccgtagcc aggaacaccg gccggaggga cgtaggtcga gcacaccagc
      181 gtcgtgtccc tgatgttgtc cttggccggg atcaacaggc tcgatcgaga gccggggatc
      241 gacccgaggt caacctctgg ggcttcccgt ccgaaggtga gatagtccag cgcccgttgc
      301 agggcggccc gggcatcggc tgactccgcg gccgaagcct ctctcgctgt caccgaagtg
      361 gcggggcgcg gcaccgtgat gtggttctcc atgagccagg gcgcgtcggc tacgggctgg
      421 acgaacaccc caaccactcg gcggtcggcc ggggaggccg acgccgtcgc gctggggctg
      481 gccgtgccac ccgctcgttc cgattcgccg aactcgatca ccgcccaccg gggataggcc
      541 ccctccggcg atgcgacgat caactccttg ggggtgaagt cgaacggcga tgccggcttg
      601 agggtcttgt tcactcgcgc atcaacagtc ccgaacaggt cctgttcgag ggtgatcccc
      661 gtgtcggcgg ccttccaccc ggtggggtcg cccgccgcgg acgcaacgat cgccgggttg
      721 ttgcggacgt tgtagtcccg caggatctgc tgcgcgagct gcgaaccgag gtctgccgcg
      781 gcggtggccg aggcagtcgg cgaaccggag gccgggtcgg tggccgatgt cgaggtcggc
      841 gaagacgaca tcgcggtgca tcccgcgagc aggagactga tcgaagcgag ggcactgcgt
      901 gccaggcgtg aggtcatggt tcgagcagat cacgatcccc accaaccccg cagagctcat
      961 ccacagccct gtggataact cagggcgccg gtggttcagc cgctggtggc cgggtgatcc
     1021 ggatgacccc ggcctcaagg tcgatcagcg gcggggcacc gtcctcgggc acctcacggg
     1081 aggactcctg acggcgcttc tcggccaggg tgtgggcgta gcccggctgc agcaacgcga
     1141 tggcttcgcc gaacacggcg ccacccgccg acgcaccctc ccgaccgaag atcacctctc
     1201 cagggtaacc gcgggcaggc gggcacgtca ccgcaaacgc cggtggggcg gcatacggga
     1261 gatcccgtat gccgccccac cggggacgtg cgtggtgtgg ctcagccctc ggcaggagcc
     1321 tcggcgtcgg ccttgtcgcc ctcgccgtcg gccatgacag cagccagcga cagcttgccg
     1381 cgcgggtcga tctctttgag ctcgacctgg accttctggc cgatcttgag gacgtcgtcg
     1441 acgttgtcga tccgcttgcc accgacaagc ttgcgcacct cggagatgtg cagcagaccg
     1501 tccttgcccg ggagcaagga gatgaacgcc ccgaaggtcg tcgtcttgac gacggtgccc
     1561 atgaagcgct cgccgacctc gggcatctgc gggttggcga tcgcgttgat cgcggcccgc
     1621 gccgcctcgg ccgaggggcc gtcgactgcg ccgatgtaga cggtgccgtc gtcctcgatg
     1681 gagatgtccg cgccggtgtc gtcctggatc tggttgatca tcttgccctt cgggccgatg
     1741 acctcgccga tcttgtcgac cggcaccttc accgtgatga tccgcggagc gaagggcgac
     1801 atctcgtcgg gagcatcgat ggcctcgccc atgacatcga ggatgtgcag ccgggcctcg
     1861 cgggcctggg tcagcgcact cgcgagcacg gaggcgggga taccgtcgag cttggtgtcc
     1921 agctggatgg cggtgacgaa ctcgcgcgtg ccggcgacct tgaagtccat gtcaccgaac
     1981 gcgtcctcgg cgccgaggat gtcggtgaga gcggcatacc gggtctcgcc atcgaccgtg
     2041 tccgagacca agcccatcgc gatgccggcg accggcgcgc gcagcggcac accggcgttg
     2101 agcagcgaga gggtcgaggc gcagaccgag cccatcgagg tcgagccgtt ggagccgagc
     2161 gcctcggaca cctgacggat cgcgtagggg aactcctcac gcgtgggcag gacgggcatg
     2221 agcgcccgct cggccagcgc accgtggccg acctcgcgcc gcttcggcga accgacgcgg
     2281 ccggtctcac cggtggagaa cggcgggaag ttgtagttgt gcatgtagcg cttgcgggtc
     2341 accggcgaga gggtgtcgag ctgctgctcc atcttgagca tgttcagcgt ggtcaccccc
     2401 atgatctggg tctcgccacg ctcgaagatc gccgaaccgt gcacccgcgg gagcacctcg
     2461 acctcggcgc ccagggcccg gatgtcgcgc aggccacggc cgtcgatgcg gaccgagtca
     2521 cgcaggatcc gctggcggac cagcttcttc tgcagggcgc ggaaggcggc ggagacctcc
     2581 ttctggcgac cggcgaattc gccgaccgtc tcgccgtcgc cgacgaactt ggcgtgcaga
     2641 cccgacttga tcgcgtcgag ggtgtcctcg cgctcggtct tgccagcaat ggtgagcgca
     2701 gctgcggtgg cgtcgtgggc gtcgccctcg accgcggcat acacgtcggc ttcgtagtca
     2761 aggaagcggg ggaactcgat ggtctccttg gccgaacgag cggccaggtc ggcctgcgcc
     2821 tcgcacagca ccttgatgaa cttcttggcg gcctcgaggc cctcggcgac gacctcctcg
     2881 gtgggggcag tcttgccctg gtgcttgacc aggtcccagg tggcctcggt ggactcggcc
     2941 tcgaccatca tgatcgcgac gtcgtcaccg acgatgcgac cggcgacgac catgtcgaag
     3001 gtcgaccgct ccgcgtcgga gaagttcggg aaggcgaccc agtggccgtc gatgcacgac
     3061 acccggacgc caccgatcgg gccgctgaac ggcaggcccg agatctgggt ggcggcgctg
     3121 gcggcgttga tggccacgac gtcgtactgg tggtcggggt tgagggcgta gaccgtgatg
     3181 atgatctgga cctcgttgcg caggcccttg acgaagctgg ggcgcagcgg ccggtcgatg
     3241 agccggcagg tgaggatggc ctcggtgctg ggacggccct cgcggcggaa gaagctgccg
     3301 gggatcttgc cgatggcgta catacgctct tcgacgtcga ccgtcagcgg gaagaagtcg
     3361 aactggtcct tgggcgtgcg cccggcggcc gtcgtggcca tgatcatcgt gtcgtcgtcg
     3421 aggtaggcga caactgcgcc gtcagcctgc ttggccaacc ggccggtctc gaaacgaatg
     3481 gtgcgggtgc cgaacttgcc gttgtcgatg gtggcttcgg cgaaaatgat ctctggaccc
     3541 tccatgggtc ctccgttctc tcttaactcc agaccgcgtg catcgtcgtt gtgcacgggt
     3601 ttgtccgtgc cggcccgctc gcgcgggtcg acatacgaga aggcggtcgc cgcagatgcg
     3661 gtgaccgcct tgtcgtggtc gttatcgacg cagacccagt cgcttgataa gcgagcggta
     3721 gcgctcgatg tcggtggcct cgaggtagcg cagcatgcgc ttgcgccgac cgaccaggag
     3781 cagcaggcca cgccggctgt ggtggtcgtg cgggtgggtg cgcgcgtgct cggtgaggtc
     3841 ctggatgcgc ttggtgagca tcgcgatctg cacctcggga gagccggtgt cgccctcgac
     3901 cgtggcgtac tcggtcatga tctgcttctt gactgtgctt tccagcggca tagggggccg
     3961 actccttctc aaggatgttg cgcggtgctc cagggcaggt ccacctgggc gctctcgatc
     4021 cgcggccgtt caacggcgtc tctcagacta ccagcgcggt ccgagccacc ctaatcggcg
     4081 gcgtatcgtc ggtgtcgtgc acgacatcca atggatcgac ctcgaagggg tcgtcaacat
     4141 gcgcgacgtc ggcgggatgc cgacgcttga cggcgggacg atcgcatcgg gccggctgat
     4201 ccgctcggac aacctgcagg agttgtccga cgccgcgatc cgccgcctcg tcgacgagtt
     4261 gggggtcacc gacgtcgtcg acctgcgcac ccacgtcgag gtcgcccagg agggcgccgg
     4321 accgctcgtc ggtcacccca gcgtccagat cagtcacttc accttgtatg ccgatgactc
     4381 ggctgagagc ggcatacccg acagcgagcg ggagctcccc tgggagacgc aggcgcgtcg
     4441 tgaggcagca gcgcgggcgc ggcgcgagca ggccaacggc gacgtccggg gcacctcggg
     4501 tgaccagtcg tccaagccgc acatcgtgcc cggcggtcag gatcacgacg acttctggtc
     4561 cgaccactac ctgagctatc tggggcaacg accggaatcc gtcgtcggag ccctgcgcgc
     4621 catcgccgac gccaagggcg ccgtcgtcgt gcactgcgcg gcaggcaagg accgcactgg
     4681 aacgatcacc ggcctggcct tggtcgtcgc cggggctcga cccgaggccg tgatcgccga
     4741 ctacgccgcg tcggccgaga gggtcccgca gatcctcgac cgcctgctgc gccgaccggc
     4801 atacgccgcg aatctgctgg gcaagacggt ggccgagcag tccccgcgtc ccgagacgat
     4861 gacccgcttg ctggccgcgt tggagcgtga cttcggtggg gtgctgggct ggttgagcac
     4921 ccaggactgg accgaggccg acaccgaagc cgtgcgcgcc aaactccgct gagccctccc
     4981 gctccggttg cccccagaac ctcacactcg ccggtgattt cgcgcagttc ggccccgacc
     5041 tcccccaagt tccccacact cgccggtgat ctcgggccca ggtatgccgc tggctcccga
     5101 cttcatcaca ctcgccggtg atctcggggc tacggcttct tcttggggtc gatgggctcg
     5161 cttgagaggg cagcgatgaa ggcttcctgc gggacttcga cccgcccgac catcttcatc
     5221 cgcttcttgc cctccttctg cttctccagc agcttgcgct tgcgggtgat gtcgccgccg
     5281 taacacttgg cgagcacgtc cttgcgcatc gcgcggatcg tttcgcgagc gatgatgcga
     5341 gcgccgatgg ccgcctgaat cggcacctcg aactgttggc gcgggatgag cttgcgcagc
     5401 ttcccggcca tctcgacgcc ataggcatag gccttgtcct tgtggacgat ggccgagaag
     5461 gcgtcgacct gctcgccctg cagcaggatg tcgaccttga ccaggtcggc gacctgatcg
     5521 ccgtccaact cgtagtccag tgacgcatat ccccgcgtgc gggacttcaa ctggtcgaag
     5581 aagtcgaaga cgatctcggc cagcggcagc gagtagcgca tctcgacgcg gtcctcggag
     5641 aggtagtcca tgccgcgcag tgccccgcgc ttgccctggc acaactccat gatcgcgccg
     5701 atgaactcgc tcggcgcgag gatcgtggcc cgaacgaccg gctcgcggac ctcggagatc
     5761 ttcccgtcgg gatactcact ggggttcgtg accgtgacga cccgccgatc atccaacgtc
     5821 acttcataga cgacgttggg ctgggtcgag atgagatcga gcccgaactc gcgctccagc
     5881 cgctcgcgga cgatctccag gtgcagcaat ccgaggaagc cacaccggaa gccgaacccc
     5941 agcgccgccg aggtctccgg ctcataggcc agcgccgcgt cgttgagctt gagcttgtcc
     6001 agggcctccc gcaggtcggg gtagtccgac ccgtcgatgg gatacaggcc ggagaagacc
     6061 atcggcttgg ggtcccgata accacccagc gactcgcggg ccggcttgcc ggcgttcgtc
     6121 acggtgtcgc cgacccggct ctggcgaacg tccttcactc cggtgatgag gtaacccacc
     6181 tcgccgaccc cgaggccctt ggacggctcg ggctccggcg aggagacacc gatctccagc
     6241 aactcgtggg tggccttggt cgacatcatg aggatccgct cgcgcgggtt gaggtcgccg
     6301 tcgatgactc ggacgtaggt gacgaccccg cggtaggtgt catagaccga gtcgaagatc
     6361 atcgcccgag ccggcgcatt cgggtcgccg accgggtgcg ggacctgctt gacgatgatg
     6421 tcgagcaact cctccacgcc gacgccggtc ttgcccgaga cctgcaggca gtccgacggg
     6481 tcgcagccga tgagacccgc gagctccgcg gcatacttct ccggctgcgc ggcagggagg
     6541 tcgatcttgt tgagcacggg gatgatcgcc aggtcgttct ccatcgcgag atagaggttg
     6601 gcgagggtct gcgcctcgat cccctgggcg gcgtcaacga gcaggacggc accctcgcac
     6661 gcggccagcg agcgagacac ttcgtaggtg aagtcgacgt gccccggcgt gtcgatcatg
     6721 ttgagcgcgt aggtcacccc gtcggactcc cacggcatcc gcacggcctg actcttgatg
     6781 gtgatgccgc gctcgcgctc gatgtccatc cggtcgagat attgcgcgcg catcgcccgg
     6841 ctgtcgacga cgccggtgag ctggagcatc cggtcagcca acgtcgactt gccgtggtcg
     6901 atgtgggcga tgatgcagaa gttgcggatg cggtccggcg gcgtgccgtg cggcggcaac
     6961 ccggtgcggg ccatgggtga cacggtggtg gggttcctcg cgggcttcgg gggtcgactg
     7021 gattcgcaac agtgtcccac gacgccgtcg gtatgccgtc cgctcacctt cggcggctag
     7081 cgtgtcgtct gtggacatcc tcgtcaccgg cggaaccggg accctcggcc gggcgaccgt
     7141 gccgctactg caagccgccg gccaccacgt gcgggtgctc agtcgccgca ccgggccagg
     7201 gctgacgacc ggggatctgg aacggcgcca aggtctcaaa cgagccctcg acggcgtcca
     7261 caccgtggtg cacctggcga cctcgggggg agctcgcgac atccgcgcgg ccgccaacct
     7321 ctcgaaggcg gcggtcgagg ccgacgtcga gcacatcatc tacatgtcga tcgtcggcgc
     7381 cgaccgggtt cccctcccct actaccaggc caaggtcgag gccgaacggc aactgcaagc
     7441 cagtggcctg ggcctgacca tccagcgcgc cacccagttc cacgaactcg tcctgacggt
     7501 gatgcgcacc cagcgggccg ccccactgtt gttcgtgcct gacatcccga ttcaaccggt
     7561 ggacctctct gctgtcgcgg ctcgcctcac ctcgctcatc ggtcaggaaa cggccgggcg
     7621 tgccccggac ctcggcggtc ctcgcgtgga gcgcttcacc gatctcgctc agcagtggca
     7681 gcaccacatc gaccggaagc gccgggtcgt ccccgtccgg ctgccggggc gcacgttcgc
     7741 ggcatacgcc cagggcgggc acctggcacc cgagggagcg gcgacg
//
LOCUS       DAOMZY010000150         7729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_8921_length_7729_cov_5.750326, whole genome shotgun sequence.
ACCESSION   DAOMZY010000150 DAOMZY010000000
VERSION     DAOMZY010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7729
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8921_length_7729_cov_5.750326"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="PLK64_09500"
     CDS             <1..1219
                     /locus_tag="PLK64_09500"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HPV80025.1"
                     /translation="SFAGGKVVTTPAADGVALQVDDAVRKVVQAWPRVESVTAAASIT
                     APKVTKAKVDAAVTAFAAPAMSGPVTIVVGDKTAVVTPEQYAEALSMVPDESGTLNPV
                     VDKEALRGVVASATSAVVVAPKDATIVLEGDKPVIRPSVDGVSVDTASAADLLVKALT
                     APDRRVSLSTVAAKATLTTEGAQALGVREVIASFDSAYPYDPPRTNNMTLGAAAVNGT
                     LIKPGEVFSLNKVLGERTTAKGYQEAGVISNNRLTKNVGGGVSQISTVTYNLAWFAGV
                     ELTAHKAHSFYISRYPAGREATVSWPDLDNKWTNNSPYGILVQMWLSGGQVHGRMWST
                     KVYDVTAVAGPRTNIKHGRSITDSSPSCVPHAFTDGFDITVQRVFSKGGSVVKRESYS
                     TTYVPADQIICSG"
     gene            complement(1258..2007)
                     /locus_tag="PLK64_09505"
     CDS             complement(1258..2007)
                     /locus_tag="PLK64_09505"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV80026.1"
                     /translation="MTDGWRALEEVWLGDWLLRASHGFTQRGNSALVIDDPGLPLAAA
                     VSTVERWYAARDLPPRFCLVTDVAGSAADPSVSTRLAQSGYVSRSATYTMTALTADLP
                     GPPRGARDVVVDDQVTPAWLAAFAAYRRLVPGVAEAILAGSHGQLFLSVPGEGSADAP
                     PIAIARMSTYPQWAGIHAMWVAPEHRREGVATVVVAAVARLAAARDLSGVYLQVERSN
                     SVATAAYDGWGFRAHHGHVYYAPADDGLGHS"
     gene            2140..2466
                     /locus_tag="PLK64_09510"
     CDS             2140..2466
                     /locus_tag="PLK64_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010523683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HPV80027.1"
                     /translation="MTYVIALPCVDLKDKACIEECPVDCIYEGERMLYIHPDECVDCG
                     ACEPVCPVEAIYYEDDVPAEWSDYYKANVEFFDDLGSPGGAAKLGLIPKDHALVSALP
                     PQASEH"
     gene            2476..3633
                     /gene="dapC"
                     /locus_tag="PLK64_09515"
     CDS             2476..3633
                     /gene="dapC"
                     /locus_tag="PLK64_09515"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HPV80028.1"
                     /translation="MPLTPPSLPDFPWDSLVPFRRIAAAHRDGIVDLSVGTPVDPTPQ
                     AIQAALVAAADSPGYPTTVGTPALREAVVAWFERRRGVGGLSPDAVLPTIGSKELVAW
                     LPTLLGVGAGDLVGIPAVAYPTYDVGARLAGAAPIVVDGLAALGPLTPSTTPKVLWIN
                     SPGNPTGAVLPVEHLAKVVAWARRHGVVVAADECYAELDWRQPDPRRPGPRHTTPSLL
                     DPAVCGGSHEGLLAVYSLSKQSNLAGYRAAFVAGDPALIAGLVEVRRHAGMMVPGPVQ
                     AAMTVALRDDEHVADQRLRYGVRREILIEALEGVGLRIEHSEAGLYLWATRDEDCWET
                     VGWFAERGILVGSGSFYGEAGRRHVRVALTATDERIAAAAGRLSVGSHREG"
     gene            3699..5000
                     /locus_tag="PLK64_09520"
     CDS             3699..5000
                     /locus_tag="PLK64_09520"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HPV80029.1"
                     /translation="MTDTETATHASLHVGARDASLQIVPSAQGEPGLNVGALLKDTGF
                     VTYDAGFVNTAACRSAITFIDGDVGILRYRGYPIEQLAEKSSYVEAAFLLIYGELPTP
                     SQLADFDSRIRHHTMLHEDLRNFFSGFPRDAHPMPVLSSAVSALSTFYQDSLDPFDPM
                     QVEISTIRLLAKLPTIAAYAYKKSIGQPFLYPDNNLSLPENFLRMTFGTPAEPYEVDP
                     VHAKALDLLLLLHADHEQNCSTSTVRLVGSSHANLFASVSAGINALFGPLHGGANQAV
                     LEMLNSLRESGESPEAFMRRVKNKEGGVRLMGFGHRVYKNYDPRASLIKKAADDVLAK
                     LGVDDPLLGLAQELEAIALADDYFVERRLYPNVDFYTGLIYKAMGFPTKMFTVLFALG
                     RLPGWIAQWREMMTDPETKIGRPRQIYTGATERDYVGIDYR"
     gene            complement(5041..5919)
                     /locus_tag="PLK64_09525"
     CDS             complement(5041..5919)
                     /locus_tag="PLK64_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80030.1"
                     /translation="MPRTPAGLILAEQGPPSPRRRRVVRVFVAALVLVVAAAGWVGVR
                     TILASLSPEACWTTSPSARVEVTPEQAGNAATISAVAVSRGLSSRATTIAIATALQES
                     KLRNLRYGDRDSVGLFQQRPSQGWGTVEQILDPVYASNAFYNKLVKVKDYLTRPLTEV
                     AQEVQRSGAPAAYAQHEDEAQALAAALTGAAPATLVCRLDPPSAVPAAKDLRAAITNE
                     FGTPTAGSGGTVTVAAEGATAWALASWAVAHADARGVSRVEVGGRTWSRSDPQTWQST
                     PSGPQDTVTITLVTAL"
     gene            complement(5934..6863)
                     /gene="dapD"
                     /locus_tag="PLK64_09530"
     CDS             complement(5934..6863)
                     /gene="dapD"
                     /locus_tag="PLK64_09530"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HPV80031.1"
                     /translation="MSERTAWGFGLATVTKHGDVLDAWFPQPSLGTPPDDVAAPTAIA
                     ELAGKDKVRKVTQAVVLVIADLDSPPADTADAYLRLHLLSHRLVAPNSINLDGIFAAL
                     PNVVWTNHGPCAVDGFEQTRARLRAQGPVTVFGVDKFPRMVDYVVPSGVRIADADRVR
                     LGAHLASGTTVMHEGFVNFNAGTLGASMVEGRISQGVVVGDGSDIGGGASTMGTLSGG
                     GTERVRIGRRCLLGAESGLGIALGDDCVVEAGLYVTAGTKVTLPDGQVVKARELSGQD
                     NLLFLRDSVSGTVVVKSRDAHGIALNAALHAND"
     gene            6962..>7729
                     /gene="dapE"
                     /locus_tag="PLK64_09535"
     CDS             6962..>7729
                     /gene="dapE"
                     /locus_tag="PLK64_09535"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HPV80032.1"
                     /translation="MPLTSPSLDLAADVVTLTAAICDIESVSGDEGPLADAIEAALGG
                     LAHLEVVRDGHTVIARTALGRPERVVVAGHIDTVPLTRHTPNLPTRRVGDELVGRGTV
                     DMKGGVAVMLKLAAALTAPNRDVTYCFYDCEEVDSERNGLRRVAEHRPELLAADFAVL
                     MEPTDGTVEGGCKGTLRVEVRTKGIASHSARPWVGHNAIHDAGEVLARLVAYEARVVE
                     VEGLTYREALQAVLISGGTASNVIPDACAVTVNYRYSP"
BASE COUNT         1186 a         2716 c         2661 g         1166 t
ORIGIN      
        1 gtcgttcgcg ggcgggaagg tcgtcactac cccggcggcc gacggcgtgg ccctgcaagt
       61 agatgacgcc gtccgcaagg tggtccaggc ctggccgcgg gtcgagtcgg tcaccgctgc
      121 cgcgtccatc accgcaccga aggtcaccaa ggccaaggtc gatgccgcgg tgaccgcgtt
      181 cgcggcaccg gcgatgtctg gcccggtgac gatcgtcgtg ggcgacaaga ccgcagtcgt
      241 cacgcccgag cagtatgccg aggcgctctc gatggtcccg gacgagagcg gcacgctcaa
      301 tccggtcgtc gacaaggagg ccctacgcgg tgtcgtcgcg tcggcgacca gcgctgtcgt
      361 cgtggcaccc aaggacgcca cgatcgtcct cgaaggcgac aagccggtca tccgcccctc
      421 ggtcgacggg gtgtcggtcg acaccgcctc ggccgcagac ctgttggtga aggcactcac
      481 cgcccccgat cggcgcgtct cgctgtcgac ggtcgcggcc aaggcgacgt tgacgaccga
      541 aggtgcccag gccctggggg tccgcgaggt catcgccagc ttcgactcgg cctaccccta
      601 cgacccgcca cggaccaaca acatgaccct cggcgcggcg gccgtcaacg gcacgctcat
      661 caagcccggt gaggtgttca gcctcaacaa ggtcttgggg gagcgaacga cggccaaggg
      721 ttaccaggag gccggcgtca tctccaacaa tcgcctgacc aagaacgtcg gtggcggggt
      781 ctcgcagatc tcgacggtga cctacaacct cgcctggttc gcgggcgtcg agctcaccgc
      841 gcacaaggcg cactcgttct acatctcgcg ctaccccgcg ggccgcgagg ccaccgtgag
      901 ttggcccgac ctggacaaca agtggaccaa caacagcccc tacggcatcc tcgtgcagat
      961 gtggctgtcc ggcggccagg ttcacggccg gatgtggagc acgaaggtct acgacgtcac
     1021 cgccgtcgcc ggcccccgga ccaatatcaa gcacggcagg tcgatcaccg acagcagccc
     1081 gtcgtgcgtg ccgcacgcct tcacggacgg cttcgacatc accgtccagc gggtcttctc
     1141 caagggcggt tccgtggtca agcgcgagtc ctactcgacg acctacgttc ccgccgacca
     1201 gatcatctgc tccggctagc tgcgcccggc tagctgtgcc cggctagctg cgcctggcta
     1261 gctgtgccca aggccgtcgt cggccggcgc gtagtagacg tggccgtggt gggcccggaa
     1321 gccccagccg tcgtatgccg ccgtggccac cgagttgctg cgctccacct ggaggtagac
     1381 gcccgacaga tcacgcgcgg ccgcgaggcg ggccactgcg gcgacgacca ccgtggccac
     1441 cccctcacgc cggtgctccg gcgccaccca catcgcgtgg atgcccgccc actgcgggta
     1501 ggtcgacatc cgcgcgatgg cgatcggcgg ggcatcggcg gagccctcgc cgggcaccga
     1561 gaggaacagt tgaccgtgcg agccggccag gatcgcctcg gcgacgcccg gcacaaggcg
     1621 tcggtatgcc gcgaacgccg ccagccaggc cggcgtgacc tggtcgtcga cgaccacgtc
     1681 acgggcaccc ctcggcgggc ccggcaggtc ggcggtaagg gccgtcatgg tgtaggtcgc
     1741 cgagcgggac acgtagcccg actgcgccaa gcgcgtggac accgaggggt cggcggctga
     1801 ccccgccacg tccgtgacca gacagaagcg cgggggcagg tcgcgggcgg cataccaacg
     1861 ctccacggtg ctgaccgcgg cggcgagcgg caggccgggg tcgtcgatga cgagcgcgga
     1921 gttgccccgc tgggtgaagc cgtgggacgc ccgcagcagc cagtcgccga gccagacctc
     1981 ctcgagggcc cgccacccgt cggtcatcac atcttctagt gccctgacgc cgatccgcac
     2041 ggctatatcc tgccttcgtc gccggggcgc aggtgtgcag cccccgtcga ctcggcatac
     2101 taggtgcgtc aacccaggcc cgtaggagga ccgctcacca tgacctacgt catcgccctg
     2161 ccatgcgtcg atctcaagga caaggcctgc atcgaggaat gtccggtcga ctgcatctac
     2221 gagggcgagc ggatgctcta catccacccc gacgagtgcg tcgactgcgg cgcctgcgag
     2281 ccggtctgcc ccgttgaagc gatctactac gaggacgacg tcccggcgga gtggtccgac
     2341 tactacaagg ccaacgtgga gttcttcgac gacctgggca gccccggcgg tgccgccaag
     2401 ttgggcctga tccccaagga ccacgccctg gtcagcgcgc tgccgccgca ggcctccgag
     2461 cactgagcga tcgccctgcc actcacgcct ccgagccttc ccgactttcc ctgggactcg
     2521 ctcgtcccgt tccgtcggat cgctgctgcg catcgcgacg gcatcgtcga cctgtcggtc
     2581 ggcaccccgg tcgacccgac cccgcaggca atccaggctg ccctcgtggc ggcggccgac
     2641 tcgccgggct acccgacgac cgtcggcacg cccgccctgc gtgaggcggt tgtcgcgtgg
     2701 ttcgagcgtc gccgcggagt ggggggtctg tcgcccgatg cggtgctccc gacgatcggg
     2761 agcaaggagc tggtggcctg gctgccgacg ttgctcgggg tcggagccgg cgaccttgtc
     2821 gggatccctg ccgtcgcgta tccgacctac gacgtcgggg cccggttggc gggagccgcg
     2881 ccgatcgtcg tcgatgggtt ggccgctctt gggccgctga ccccgtcgac cacccccaag
     2941 gtgctctgga tcaacagccc cgggaacccg acgggcgccg tcctgccggt cgaacacctg
     3001 gcgaaggtcg tggcgtgggc acgtcgccac ggcgtcgtcg tcgcggcgga cgagtgctat
     3061 gccgagttgg actggcgaca gcccgacccc cgtcgccccg ggccacggca tacgaccccc
     3121 tccctgctgg accctgcggt gtgcggcggc agccatgagg ggcttctcgc ggtctactcg
     3181 ctgtccaagc agtccaacct cgccggctac cgggcggcgt tcgtggccgg tgacccggcc
     3241 ctcatcgccg gcctcgtcga ggtgcgcagg cacgccggga tgatggtgcc tggccccgtc
     3301 caggcagcga tgacggtggc gctgcgggac gacgagcatg tggccgacca acggctgcgc
     3361 tacggcgtgc ggcgagagat cctcatcgag gcgcttgagg gcgtgggtct gcggatcgag
     3421 cattcagagg cgggcctcta cctctgggcg acccgcgacg aggactgctg ggagaccgtg
     3481 ggctggttcg ccgagcgggg gatcctcgtc ggctcgggct ctttctacgg tgaggctggt
     3541 cgccgccatg tgcgcgtggc gctcactgcg accgacgagc gcatcgccgc tgccgccggt
     3601 cgcctgtctg tagggtcaca tcgcgagggc tgacgtgctc aggtaacgtc cggttgagcc
     3661 accgggatga gccatgttca cctgacgaag ggatggtcat gaccgacacc gagaccgcga
     3721 cccacgcctc gcttcacgtc ggcgctcgtg acgcgagcct gcagatcgtg ccgtccgccc
     3781 agggagaacc cggcctcaac gtcggcgcgc tgctcaaaga caccgggttc gtgacctatg
     3841 acgcgggctt cgtcaacacg gcagcctgcc gcagcgcgat cacctttatc gacggtgacg
     3901 tcggcatcct gcgttaccgc ggctacccca tcgagcagtt ggccgagaag tccagctatg
     3961 tcgaggcggc cttcctgctc atctacggtg agctgcccac gccgagccaa ctcgccgatt
     4021 tcgactcgcg gatccggcac cacacgatgt tgcacgagga cctgcgcaac ttcttctccg
     4081 gtttcccgcg cgatgcccac ccgatgccgg tcctctcctc ggccgtgagc gccctgtcga
     4141 ccttctacca ggactccctc gacccgttcg acccgatgca ggtcgagatc tcgacgatcc
     4201 gtctgctggc caagttgccg acgatcgccg cctacgccta caagaagagc atcggccagc
     4261 cgttcctcta ccccgataac aacctgtcgc tgcccgagaa cttcctgcgg atgaccttcg
     4321 gcacgcccgc cgagccctac gaggtcgacc cggtccacgc gaaagccctg gacctccttc
     4381 tgctcctgca cgcagaccac gagcagaact gctcgacctc aacagtccgg ctggtcggtt
     4441 cgtcgcacgc caacctcttc gcgtcggtat ccgccggtat caacgcgttg ttcggcccgt
     4501 tgcacggcgg tgccaaccag gccgtgctcg agatgctcaa cagccttcgc gagtccgggg
     4561 agtcacccga ggcgttcatg cgccgagtga agaacaagga gggcggggtc cgactgatgg
     4621 gcttcggcca ccgggtctac aagaactacg acccccgagc ctcgttgatc aagaaggccg
     4681 ctgacgacgt cctggccaaa ctcggagtgg acgacccgct gctgggcctg gcccaggagt
     4741 tggaggcgat cgccctcgcg gacgactact tcgtcgagcg ccggctttac cccaacgtcg
     4801 acttctacac ggggctcatc tacaaggcga tgggctttcc gaccaagatg ttcacggtcc
     4861 tgttcgctct cgggcggctg cccggctgga tcgcccagtg gcgcgagatg atgactgacc
     4921 ccgagaccaa gatcgggcgg ccgcggcaga tctacaccgg tgccaccgag cgggactacg
     4981 tcgggatcga ctaccgctga ccacgccggg ccctcgaccg gcgcaccagg ccggtcgagt
     5041 tcagagcgcc gtcaccaggg tgatggtgac cgtgtcctga gggccgctcg gcgtcgactg
     5101 ccaggtctgc gggtcgctcc gtgaccaggt ccggccgccc acctcgacgc ggctcacccc
     5161 acgcgcatcg gcgtgggcga cggcccacga cgccagcgcc caggcggtgg ccccctcggc
     5221 ggccaccgtg accgtcccgc cggaccccgc cgtcggcgtg ccgaactcgt tggtgatcgc
     5281 cgccctcagg tccttcgccg cagggacggc ggacggcggg tcgaggcggc ataccaaggt
     5341 cgcaggcgcc gcgcccgtca gtgcggcggc gagagcctgt gcctcgtcct cgtgctgggc
     5401 gtatgccgct ggcgcaccac tgcgctggac ctcctgagcc acctcggtga gcggccgagt
     5461 gaggtagtcc ttgaccttga cgagcttgtt gtagaacgcg ttggaggcat agacggggtc
     5521 gaggatctgc tccacggtgc cccagccctg gctcgggcgc tgctggaaga ggccgaccga
     5581 atcgcggtcg ccgtagcgca ggttgcgaag cttcgactcc tgcagggcgg tcgcgatggc
     5641 gatggtggtg gcgcggctgg acaggccgcg ggacacggcg acggcggaga tggtcgcggc
     5701 gttgccggcc tgctcggggg tgacctcgac ccgggccgac ggcgacgtcg tccagcaagc
     5761 ctccgggctg aggctcgcga ggatcgtgcg caccccgacc cagccagcag cggccacgac
     5821 gagcacgagg gcagcgacga acacgcgcac gacccggcgg cgacgaggag agggtggacc
     5881 ttgttcggcg aggatcaggc ccgcgggggt gcgcggcata cggggtcgtc cggtcagtcg
     5941 ttggcgtgca acgcggcgtt gagagcgatc ccgtgagcgt cgcgcgactt gacgacgacc
     6001 gtgcccgaga ccgagtcacg caggaagagg aggttgtcct ggccggacaa ctcgcgcgcc
     6061 ttgacgacct ggccgtcggg cagggtgacc ttggtgccgg ccgtgacata gaggccggcc
     6121 tcgacgacac aatcgtcgcc gagcgcgatg cccaggccgg actccgcgcc gagcaggcag
     6181 cgccgcccga tccggacccg ctcggtgccg ccgccggaca gggtgcccat cgtcgacgcc
     6241 ccgccgccga tgtcggaccc gtcgccgacg acgacgccct gggagatccg gccttcgacc
     6301 atcgaggcgc ctagcgtgcc ggcgttgaag ttgacgaagc cttcgtgcat gaccgtcgtg
     6361 ccagaggcga ggtgggcacc cagccggacc cgatcggcgt cggcgatccg cacacccgac
     6421 ggaacgacgt agtcgaccat ccgggggaac ttgtcgacgc cgaagaccgt caccggcccc
     6481 tgggcgcgca gccgcgcccg ggtctgctcg aagccgtcga ccgcgcacgg cccgtggttg
     6541 gtccagacga cgttgggcag ggcggcgaag atgccgtcga ggttgatcga gttgggtgcc
     6601 acgagccggt ggctgagcag gtgcagacgc agataggcgt cggcggtgtc ggcaggcggc
     6661 gagtcaaggt cggcgatgac gagcaccacg gcctgcgtga ccttgcggac cttgtccttg
     6721 ccggcgagtt cggcgatcgc ggtgggcgcg gcgacatcgt cgggcggcgt gcccaaggac
     6781 ggctgtggga accatgcgtc caacacgtca ccgtgcttgg tcaccgtggc caacccgaat
     6841 ccccacgccg tgcgctcgct catgcgtcaa gcctaggcga gtgggcggac gtcagccgcc
     6901 acgcccacac cagccgtcgc gggttggagc gggcgagcgg cataccccta gcctgagggg
     6961 tatgccgctc acctcgcctt cgctggacct tgccgccgac gtcgtcaccc tcaccgctgc
     7021 catctgcgac atcgaatcgg ttagtggcga cgagggcccg ttggccgatg ccatcgaggc
     7081 ggccctgggc gggctggccc accttgaggt cgtccgcgac ggtcacaccg tcatcgcccg
     7141 gacggcgctg gggcgcccgg agcgggtcgt cgtcgccggg cacattgaca cggtgccgct
     7201 cacgcggcat acccccaacc tgccgacccg acgcgtgggc gacgagttgg tcgggcgtgg
     7261 gaccgtcgac atgaagggcg gggtggccgt catgctcaag ttggcggcgg ctctgaccgc
     7321 gcccaaccgc gacgtcacct actgcttcta cgactgcgag gaggtcgact cggagcgcaa
     7381 cggcctgcgc cgggtcgccg agcaccgacc cgagttgctc gccgccgact tcgccgtgct
     7441 catggagccg accgacggca ccgtggaggg tgggtgcaag gggacgttgc gggtcgaggt
     7501 gcgcaccaag gggatcgcct cgcactcggc ccggccgtgg gtgggacaca acgcgatcca
     7561 cgatgctggc gaggtgctcg cgcggctggt ggcgtatgag gcgcgggttg tcgaggtcga
     7621 ggggctgacc tatcgcgagg cgctccaggc cgtgctcatc tccggcggga cggcctccaa
     7681 cgtcatcccc gacgcgtgcg cagtgacggt gaactaccgt tactcgccc
//
LOCUS       DAOMZY010000151         7688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_8979_length_7688_cov_5.874361, whole genome shotgun sequence.
ACCESSION   DAOMZY010000151 DAOMZY010000000
VERSION     DAOMZY010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7688
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8979_length_7688_cov_5.874361"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PLK64_09540"
     CDS             <1..427
                     /locus_tag="PLK64_09540"
                     /inference="COORDINATES: protein motif:HMM:NF018479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HPV80033.1"
                     /translation="FRSRSRLWGNGHIPLFIAVVAVGGGLHLAAYAIDGRSHLGPVPT
                     LLAIAIPLAAYAVLIYALFGSLTRALTRRHLWQLAASLAVALLSVVLVALGSPLSVGL
                     VVLSFVPWVTVIAQERDARRKELSSDALALGASPHHRRV"
     gene            complement(510..1979)
                     /locus_tag="PLK64_09545"
     CDS             complement(510..1979)
                     /locus_tag="PLK64_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPV80034.1"
                     /translation="MMTGFGGATADREETTAMTDQTATQAWPAHDPATRRWLPAASTP
                     VDPGRVEALLAAEWERFVRQTPASAEHNARASKSLPLGVTSSFQHWDPYPISIVTARG
                     AHVTDVDGRRLLDMSMGFGAMMVGHLHPTVVEHVRSALDTVGTLFVTPSPTATEVAEL
                     FQDRFQLDMLRFTQSGTESVMYAVRVARAYTGRKAIVKIEGGYHGGYDALQVSVKPAL
                     ADIGPADAPVPSVPHDAEAGTVYVVPFNDLAYLRRILAEHGREIAALVMEPVIENLAI
                     VVPDEGYLAGVRAACDEFGVVLIFDEVKTGLTAGYAGAAQRLGVKPDLITLAKSIGGG
                     LPLAAFGGRQEVMQVVVDGRMHHFGTYNGNPLVMAAARAVDEICTRDALQGAEDINVR
                     ALDAVDAVIEEFELPAHTVGLGIKGCVTWSTTPVRNYRDYKATDFRMAELSWLWGVNR
                     NILTPPGLDEQWLVSLAHTDEDMAALVSDFRDLAVALRA"
     gene            2212..2604
                     /locus_tag="PLK64_09550"
     CDS             2212..2604
                     /locus_tag="PLK64_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HPV80035.1"
                     /translation="MNGSTSFWDLLLWSFWIFIWISAIIIWFRCVFDLFTDHTLSGWG
                     KAGWAALLIFLPWLGAFIYIVARGRSMAQRQVDAAVSAVAQQEKYIRSVASSSISPTE
                     QIMHAKSLLDAGAISQDEFASLKAKALA"
     gene            complement(2607..3932)
                     /locus_tag="PLK64_09555"
     CDS             complement(2607..3932)
                     /locus_tag="PLK64_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80036.1"
                     /translation="MPGTDGHDEHPILSDKDDAEQRGENPPGSFGHTKRPSGDLDPNW
                     QPSDRVRGEAEELIKGFGREHRPRREDVLPYLLIRAYSPGDRGVRPTWPPRPCWESPD
                     LLLIDAAYTGPFTPARLVASPTAGRSYRVFVRVWNLGLFPAIGVHVRAWFVNPGFFGG
                     DPSNPAYAPQLIGGAMVHLEDRTRPGAMQLVELDRAWDIPAALTGHECLMASVSCPAD
                     QWSGALDANHDRHVGQRNLNILAGPMSAKDLIFGLGAMVTGTGTLELIHGGPAVTPLL
                     RALAGREKTEFGALGALGAPLAKTLLRGVPIGTGQQHLLTMFRTQKGWLVADSAKVLT
                     AGLEFGVIDKRSLGRGRPHPFATPLTTRRVIEELGVERADFFGVLTDLEPGQALVEGL
                     VMLWGLGDLGAANLAHALAEGKPAAHLLRFAHHDGELREDGGYSAVVIG"
     gene            complement(3938..6361)
                     /locus_tag="PLK64_09560"
     CDS             complement(3938..6361)
                     /locus_tag="PLK64_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80037.1"
                     /translation="MPGWYVHMEAAHDTARSMRNGTIPPGFPISPAEAMNIGNHCHTW
                     RNYLALGSLGPDLFYLLPDFKNTTGQVIRQVVQWCLDVWEVVDSEFVSKWEKWIDPIS
                     TNNSQLASQLTGGLSTQLAQVLDELTSAILSAFKGLLAQMGDWFGVLTSGVPQGFGDD
                     AFYWSDMFHYRKTYQFPFVLFQQAQAALASATTDDERADAQARIAFAVGWMSHCATDV
                     TGHPYTNAKSGGPYRDHWQRHHLVENHMDSENYVARHPGSLYAEYGTSALHFRLAFRH
                     RNDAPYNGRDDAPAYDYWTGFPAYDNGDGPTPTANRHTFFDLDTGPLPGHLVDGLLEA
                     MADVYGVDGPHILMQDPPFSATDPGTGQPDGRPNEPAMAQMWEIVYKYLKMTSSDAIS
                     LRLPPPPSVFTDHSFPTPPGGGGSGIDDDPSRGADVDDDDSFNLLDLLLALFAWIVYL
                     AQVVVWLLTILPGLILDITTFPAREVIYWAVIVPAWNLYLLARRALVMSGFAMPKPAE
                     ISFGLTTLGNEGSYDIGSALDSPLGIGGGPRPITEPSGRLNATDARGLDLAYPRGVVR
                     DLPSAISRPDLVGALGLTSPLRYAADAPSEEFKASEWIAPWRYPLTNQAGAGVAQEGA
                     ATHVGPYVVGSNSTVLLSGMVGDNGARAELEKAGSPAETEAVLDGRLPLNQHLGAPID
                     YGLYLVGRMADEVGNADFGVPDFNLDSDRGYAWHCWDWDRSDIPCVPDINPAFGEDYG
                     YPLPCTSPQMFHADHDCPPAPDRWYDESLDLAVHYLPGKGPDTCKPGTPHKPNTDPSW
                     LDRAKHGRG"
     gene            complement(6503..7120)
                     /locus_tag="PLK64_09565"
     CDS             complement(6503..7120)
                     /locus_tag="PLK64_09565"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPV80038.1"
                     /translation="MTHELRDHWDRAYALGDHTRSWFQEEAAPSVAAFDAAGVTPADS
                     VIDVGGGASRLVEGLLDLGFDDVTVLDVSPAAIQIARTRAGLRAARVTWLVTDLLEWI
                     PTRTYAVWHDRAVLHFLVTDEDQRRYAAALDRATEPGSVAVIAGFAPDGPTQCSGLPT
                     ARHDEVSLAALVGPKWELASSGREVHRTPNDAEQSFLWTTFRRIG"
     gene            7195..>7688
                     /locus_tag="PLK64_09570"
     CDS             7195..>7688
                     /locus_tag="PLK64_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPV80039.1"
                     /translation="MPRAERSGAAYPLGYMMERRTRPDRPLGQPKEAAAMFFTQYYLD
                     CLSQASYLVGDASSGLAVVVDPRRDVADYLADARAAGLRIVGVINTHFHADFVSGHLE
                     LAAETGAWIGYGEAATPEFTHRPLADGERISLGDVTLQIMATPGHTPESISVLVFEHA
                     HDDVA"
BASE COUNT         1224 a         2523 c         2743 g         1198 t
ORIGIN      
        1 cttccgcagc cggtcccggc tatggggcaa cggccatatc cccctcttca tcgcggtcgt
       61 ggccgtcggc ggcggactgc acctcgcggc atacgccatc gacggacggt cgcatctggg
      121 tccggtgccg accctcctcg cgatcgcgat ccccctcgcc gcgtatgccg tgttgatcta
      181 cgcgctgttc ggctcactga cgcgggccct cactcgcagg catctgtggc agttggccgc
      241 gtcgctcgcg gtggcgctgc tcagcgtggt cctggtggcg ctgggctcgc cgttgtcggt
      301 ggggctggtc gtgctctcgt tcgtgccgtg ggtgaccgtg atcgcgcagg agagggacgc
      361 ccgtcgcaag gaactcagca gtgacgcctt ggccctgggg gcttccccac atcaccggcg
      421 ggtgtgaggg aatccgggac gccagggccc ggcgagctgg aaatcaccgg cgggtgtgag
      481 gaaatccgga aagagggcgg ggcagcgggt caggcgcgga gtgccaccgc cagatcgcgg
      541 aagtcgctga ccaacgccgc catgtcctcg tcggtgtgcg ccagcgaaac cagccactgc
      601 tcgtccagcc cgggcggcgt gaggatgttg cggttgacgc cccagagcca ggacaactcg
      661 gccatccgga agtcggtcgc cttgtagtcg cggtagttgc gcaccggagt cgtcgaccag
      721 gtgacgcagc ccttgatgcc caaccccacc gtgtgcgccg gcaactcgaa ctcctcgatc
      781 acggcatcga cggcgtcgag ggcacggacg ttgatgtcct cggccccctg caacgcatcc
      841 cgcgtgcaga tctcgtccac cgcgcgggcg gcggccatga ccagcgggtt gccgttgtag
      901 gtgccgaagt ggtgcatccg accgtcgacg acgacctgca tgacctcctg ccgacccccg
      961 aacgcggcca gcggaagccc gccgccgatg ctcttggcca gcgtgatgag gtcgggcttg
     1021 acgccgaggc gctgagcggc accggcatac ccagcggtga gcccggtctt cacctcgtcg
     1081 aagatgagga cgacgccgaa ctcgtcacag gccgcgcgca ccccggcgag gtaaccctcg
     1141 tcagggacga cgatggcgag gttctcgatg accggctcca tgacgagggc ggcgatctcg
     1201 cggccgtgct cggcgaggat ccgacgcagg taggcgaggt cgttgaacgg cacgacgtag
     1261 acggttcccg cctcggcatc gtgcggcacc gacggcaccg gcgcgtcagc cggcccgatg
     1321 tcggccagag ccggcttgac cgacacctgc agcgcgtcgt acccaccgtg gtaaccgcct
     1381 tcgatcttca cgatcgcctt gcggccggtg taggcgcgag ccacgcgcac ggcatacatc
     1441 acggactccg tgcccgactg ggtgaagcgg agcatgtcga gttggaagcg gtcctggaac
     1501 aactcggcca cctccgtcgc ggtcggcgac ggcgtgacga agagggtgcc cacggtgtcg
     1561 agggcgctgc ggacgtgctc gacgacggtc gggtgcaggt gaccgaccat catcgcgccg
     1621 aagcccatcg acatgtcgag caagcgccgc ccgtcgacgt cggtgacgtg cgccccgcgc
     1681 gccgtgacga tcgagatggg gtaggggtcc cagtgctgga aggacgacgt gacaccgagc
     1741 ggcaggctct tgctcgcgcg ggcattgtgc tcggccgagg ccggggtctg gcggacgaac
     1801 cgctcccact cggcggccag cagggcttcg acccggccgg gatccaccgg cgtggatgca
     1861 gcgggcagcc accggcgggt cgccgggtcg tgcgcgggcc atgcctgggt cgcggtctgg
     1921 tcggtcatcg ccgtcgtctc ctcacggtcg gcggtcgccc cgccgaatcc ggtcatcatt
     1981 gcctatccgt cgccagattc ctagtcgaca gtagaaaacg actcccagcc acgcatgaga
     2041 cgacggattc cgtatgccgc gctgcagctc cgcgccacgg ctgcgctgcg cgctgcggcg
     2101 acgctccgtg ccgaccggct ggccgagcgg cggtgggtgt acgcatcctg agcgcaaagc
     2161 cagcaccggg gctactctcg aaagaccacc atttcgagag gagtgacgac gatgaacggc
     2221 agcaccagct tctgggatct tctcctgtgg agcttttgga tcttcatctg gatctcggcc
     2281 atcatcatct ggttccgctg tgtgttcgac ctcttcaccg accacaccct cagcggctgg
     2341 ggcaaggccg gttgggccgc cctgctgatc ttcctgccgt ggctcggcgc cttcatctac
     2401 atcgttgcgc gcggccgcag catggcccaa cgtcaggtcg acgccgcggt ctctgcggtg
     2461 gcccaacagg agaagtacat ccggtcggtc gcgagcagct cgatcagccc gacggagcag
     2521 atcatgcacg ccaagtcgct cctcgacgcc ggcgcgatca gccaggacga gttcgcctcc
     2581 ctcaaggcca aggctctggc ctaaactcag ccgatcacga cggccgagta gccaccgtcc
     2641 tcacggagct cgccgtcgtg gtgagcgaaa cgcagcaggt gcgctgctgg cttgccctcg
     2701 gcgagggcgt gagcgagatt ggccgcgccc aggtcgccca ggccccaaag catgaccaga
     2761 ccctcgacga gcgcctggcc cggctcaagg tcggtcagca cgccgaagaa gtccgcccgc
     2821 tcgacgccga gttcctcgat gacccgccgg gtggtcagcg gcgtggcgaa ggggtgcggc
     2881 cggccgcggc cgagggaacg cttgtcgatg accccgaact ccagcccggc cgtgagcacc
     2941 ttggcgctgt cggccaccag ccaacccttc tgggtccgga acatcgtcag cagatgctgc
     3001 tgacccgtcc cgatcgggac cccacgaagg agggtcttgg ccaacggagc acccaaagcc
     3061 cccaacgctc cgaactcggt cttctcccgc ccagccagcg cacgcagcag cggcgtgacc
     3121 gccggcccgc catggatcag ctcgagcgtc cccgtcccgg tcaccatcgc ccccagaccg
     3181 aagatcaggt ccttggcgct catcggcccg gcgaggatgt tgaggttgcg ctgcccgacg
     3241 tgccggtcat ggttggcatc cagcgcacca ctccactgat cggcggggca actcaccgag
     3301 gccatgaggc actcgtgccc cgtcagcgcc gcagggatgt cccacgcccg atccagttcg
     3361 acgagctgca tcgcaccggg ccgggtccga tcctccaggt gcaccatcgc tccgccgatc
     3421 aactgcgggg cgtatgccgg gttgctcgga tccccgccga agaagccggg attcacgaac
     3481 cacgcccgca catgcacgcc aatcgcgggg aacagcccga ggttccagac ccggacgaac
     3541 acccggtagc tgcggcccgc ggtcgggctc gccaccagcc gcgcgggggt gaacggcccg
     3601 gtgtatgccg cgtcgatgag caggaggtca ggagactccc agcacggtcg gggcggccag
     3661 gtcgggcgga ccccacggtc gcctgggctg taggcccgga tcagcaggta gggcaggacg
     3721 tcctcgcggc gcggccggtg ctcgcggccg aaccccttga tgagctcctc ggcttcaccg
     3781 cgcacccgat cggacggctg ccagttcggg tcgaggtcgc cgctgggtct cttggtgtgc
     3841 ccgaacgatc cgggcgggtt ctcgccgcgc tgctcggcgt cgtccttgtc ggagaggatc
     3901 gggtgctcgt cgtggccgtc ggtgccaggc atggcggtca gccccttcca tgcttggcgc
     3961 ggtccagcca cgacgggtcg gtgttgggct tgtggggagt gccgggcttg cacgtgtccg
     4021 gccccttgcc gggcaggtaa tgcactgcga gatcgagcga ttcgtcgtac cagcggtctg
     4081 gggccggcgg acagtcgtga tcggcgtgga acatctgcgg cgacgtgcac ggcagggggt
     4141 agccgtagtc ctcaccgaag gccgggttga tgtcggggac gcacgggatg tcgctgcggt
     4201 cccaatccca gcagtgccag gcgtatccgc ggtcggagtc gaggttgaag tcgggcaccc
     4261 cgaagtcggc gttgccgacc tcgtcggcca tccgcccgac aaggtagagc ccgtagtcga
     4321 tcggcgcccc gaggtgttgg ttcagcggca gtcggccgtc gagaaccgct tcggtctcgg
     4381 cggggctgcc cgccttctcc aactcggcac gagcgccgtt gtcgcccacc atcccggaca
     4441 gcaggacggt cgaattcgag cccacgacgt aggggcccac gtgggtcgcc gccccctcct
     4501 gagcgacccc cgcccctgct tggttcgtca aggggtagcg ccagggggcg atccactcgg
     4561 acgccttgaa ctcctcggaa ggtgcgtctg cggcataccg gagtggggag gtgagcccca
     4621 gcgcgccgac caggtcgggt cgactgatcg cggacgggag gtcacggacg acgccacggg
     4681 ggtaggcgag gtcgaggcct cgagcgtcag tggcgttgag ccgaccggac ggctcggtga
     4741 tcggccgcgg gccgccgccg atccccagcg gcgagtcgag cgccgagccg atgtcgtagc
     4801 tgccttcgtt gccgagggtc gtcaggccaa agctgatctc ggccggtttc ggcatcgcga
     4861 agccagacat cacgagcgcg cggcgggcca gcaggtagag gttccacgcc gggacgatga
     4921 cggcccaata gatgacttcg cgagccggga aggtcgtgat gtcgaggatg aggccgggca
     4981 ggatcgtcag cagccagacg acgacctggg cgagatagac gatccacgcg aagagggcga
     5041 gcagcaggtc caacaggttg aaggagtcgt catcgtcgac gtcggcgccg cgactggggt
     5101 catcatcgat ccccgacccg cccccgccgg gaggcgtcgg gaacgagtga tcggtgaaca
     5161 ccgagggcgg cggcggcagg cgcaggctga tcgcgtcgct gctggtcatc ttgaggtact
     5221 tgtagacgat ctcccacatc tgcgccattg ccggctcgtt cgggcgaccg tcgggctgcc
     5281 cggtccccgg gtcggtcgcc gagaacggcg ggtcctgcat gaggatgtgt ggcccgtcga
     5341 ccccatagac atcggccatc gcctcgagca gtccgtcgac gagatgcccc ggcaagggcc
     5401 cggtgtccag atcgaagaag gtatgccggt tcgccgtcgg cgtgggcccg tcaccgttgt
     5461 cgtaggccgg gaaaccggtc cagtagtcgt aggcgggagc gtcgtcccgg ccgttgtagg
     5521 gagcgtcgtt tcggtgccgg aaggccagcc ggaagtgcag cgcggaggtg ccgtactcgg
     5581 catacaggga gccggggtgg cgagccacgt agttctcgga gtccatgtgg ttctcgacga
     5641 gatggtgacg ctgccagtgg tcgcggtagg ggccaccgga cttggcgttg gtgtaggggt
     5701 ggccagtgac gtcggtggcg cagtgcgaca tccagccgac cgcgaaggcg atccgggcct
     5761 gggcgtcggc gcgctcgtcg tcggtggtgg cggacgcgag cgcggcctgc gcctgctgga
     5821 agaggacgaa cgggaactgg taggtcttgc ggtagtggaa catgtccgac caatagaagg
     5881 cgtcgtcgcc aaacccctgc ggcacaccgg aggtcagcac gccgaaccag tcgcccatct
     5941 gcgcgagcag ccccttgaac gcggacaaga tcgccgaggt gagctcgtcg aggacctggg
     6001 cgagctgggt cgagaggccg ccggtgagtt gggaggcgag ttgggagttg ttcgtcgaga
     6061 tcgggtcgat ccacttctcc cacttgctga cgaactcgct gtcgacgacc tcccagacgt
     6121 cgaggcacca ctggacgacc tggcggatca cctggccagt cgtgttcttg aagtcgggca
     6181 gcaggtagaa gaggtccggc ccgagggagc cgagcgcgag gtaattgcgc caggtatggc
     6241 agtggttgcc gatgttcatc gcctcggcag ggctgatggg aaagcccgga gggatcgtgc
     6301 cgttccgcat cgatcgggcg gtgtcgtggg ccgcttccat gtggacgtac caaccgggca
     6361 tggtgactcc tctgaagcgg aaccgtgcgg gagagtgcgg gagagtgcgg gagagtgcgg
     6421 gagagtgcgg gagagtgcgg gagagtgctg gacaagccct gatggagtga aggagtcccg
     6481 gtatgccggc cagatacgcc ggctagccga tgcggcggaa agtggtccag aggaaggact
     6541 gctcggcatc gtttggagtg cgatgaactt cgcgcccgct ggaggccaac tcccatttcg
     6601 gcccgacaag cgcggccagg gagacctcgt cgtgccgagc cgtgggcagc ccggagcact
     6661 gagtcgggcc gtcgggggcg aaaccggcga tgacggccac gctgcccggc tcggtggcgc
     6721 gatccagcgc ggcggcatag cggcgttgat cctcgtcggt gacgaggaag tgcaggaccg
     6781 cgcggtcgtg ccacacggca tacgtgcggg tcgggatcca ttcgagcagg tcggtgacca
     6841 gccaggtgac ccgagccgcg cgcagaccgg cacgcgtgcg ggcgatctgg atggcggccg
     6901 gcgagacgtc aaggaccgtg acgtcgtcga agccgagatc gagcaggccc tcgacgagtc
     6961 gggaggcacc gccaccgacg tcgatgaccg aatcggccgg tgtcactccg gccgcgtcga
     7021 aggcggccac cgagggggcg gcctcctcct ggaaccaact gcgcgtgtgg tcaccgagcg
     7081 cgtaggcccg gtcccagtga tcacgtaact cgtgcgtcac atctgcaggt tagccccgcg
     7141 agaggccgtt gggtatgccc cgggcaggat gggtatgccg cggacaggct gggtatgccg
     7201 cgcgcggaac gatctggcgc ggcatacccc ctggggtata tgatggagcg gcgcacccgc
     7261 cccgaccgac cgctgggcca gccgaaggag gccgccgcga tgttcttcac gcagtactac
     7321 cttgattgct tgtcgcaggc gtcctacctc gtgggcgacg cgtcgagcgg cctggccgtc
     7381 gtcgtggatc cgcgccgcga tgtggccgac tatctcgccg acgcccgggc ggcagggctc
     7441 cgcatcgtcg gggtcatcaa cacccacttc cacgccgact tcgtctcggg gcatctggag
     7501 ctggcggccg agactggcgc atggatcggc tacggcgagg cggccacccc ggagttcacg
     7561 caccgaccac tggccgacgg agagcggatc agcctcggtg acgtgaccct gcagatcatg
     7621 gccacccccg gtcacacccc cgagtcgatc agcgtcctgg tgtttgagca cgcccacgac
     7681 gatgtggc
//
LOCUS       DAOMZY010000152         7671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_9006_length_7671_cov_5.539259, whole genome shotgun sequence.
ACCESSION   DAOMZY010000152 DAOMZY010000000
VERSION     DAOMZY010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7671
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9006_length_7671_cov_5.539259"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PLK64_09575"
     CDS             <1..219
                     /locus_tag="PLK64_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HPV80040.1"
                     /translation="DPASAPQPQILALGAVFAAMALVGDSVWALVAGRARDWFARSPR
                     RLERLGAGGGALMIGLGLSVAVAGRPTT"
     gene            332..407
                     /locus_tag="PLK64_09580"
     tRNA            332..407
                     /locus_tag="PLK64_09580"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:365..367,aa:Thr,seq:tgt)
     gene            complement(445..1626)
                     /locus_tag="PLK64_09585"
     CDS             complement(445..1626)
                     /locus_tag="PLK64_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HPV80041.1"
                     /translation="MSKARLVITAVVEQHRPVREVAASYGVARSWVYELLARWRVEGE
                     AAFEPRSRRPHSCPAAVSEQAVAAVLAERDRLVAGGHDAGPETIHWHLNQSGMTPPSR
                     ASIARILTTHGRVVPAPKKKPKTAYHRFAAEQPNECWQSDFTHYRLANGVEVEVITWL
                     DDHSRMALHISAHPRVTGPVVLATFRATINEYGCPAATLTDNGMVYTVRHSAWGVRGG
                     RNAFETELATLGITQKNGRGNHPQTQGKVERFQQTLKTWLARQPTQPATLTDLQALLD
                     TFRSQYNHHRPHRALDRRTPAAAYTARPKATPAAEPPDRTHTRVRTDKVNNGKITLRH
                     GGTLYAIGIGRSHNGKPVKALIHGLNITISDATTGEILRHLTLDTTHRYQPQTTRQGE
                     P"
     gene            1941..2945
                     /locus_tag="PLK64_09590"
     CDS             1941..2945
                     /locus_tag="PLK64_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80042.1"
                     /translation="MDTVQLERLMADALGARVDVRVQEVADRRGRFTATIQAVLVEVE
                     WLGVGWPRQVRELLDRPDRPDLVVAPEFSPGAHKLLNQARMSWIDLTGAANIGLPGLL
                     IIVESKRGARMPRASLGWTAGALSVCEGLLVGAGGTVAELVDATGLAPSTVASALKFL
                     QGEHMLSAEAARGRNARRTIVDRDQILDAYAAAAARLRSPASIRVGVLWRSPARGATE
                     LGSRLEANGVAWATTGALAADVLAPYLTEVDPWEIYVDGGSLAELRHIAREVGLAEAS
                     GGRLLLRPFPTPAKDALSSEVGGHRVAAWPRVFSDLRMIGVRGEEAAEHLRERMGIGE
                     "
     gene            3451..4476
                     /locus_tag="PLK64_09595"
     CDS             3451..4476
                     /locus_tag="PLK64_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80043.1"
                     /translation="MAFFRRSKGAPTAAPPQHPVSSFWHWWAAEGHQIDPTKPSKATD
                     ALSERLGVIHPDLTWQFGAGEGSRHCLTVSSGGVAQVRPLAERWLRAAPRPDETWEFR
                     SSQQRDPNALTNVLEIAGHRLDLSDTRFHVESDADDLRIHVGVHHPAYASLAEEVRGQ
                     VTFLLLDWLLGEDDVERWVGHVESLTEAPAGLVIGREVVAAVSDIAAQREPDEWTMGQ
                     WEDRDGAPGLAMFRRGVRWIDHPMLDRHQVLSAGYAAQDNGLPADSTVLDQLRHVESE
                     LESVLGSRGLIVAIESHRGVRTWHAYTDGDDQNVDAALAEAAPRWGATLAARPDPAWT
                     EVRQFTG"
     gene            4596..5222
                     /locus_tag="PLK64_09600"
     CDS             4596..5222
                     /locus_tag="PLK64_09600"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPV80044.1"
                     /translation="MDLSFPLATIAPTLDAGVLHVLAATTSGCTGAEVHRRLGHGSDE
                     GVRKVLARLVGQGIVVVETPARYPLYRLNRSHVAAPHIEALTRVRQDIVDRIRDQVTA
                     WQVLPTHAGLFGSFARGTAHANSDIDVLLVRPETLTEREEDLWLDQVDGLDRAIKAWT
                     GNVAQIIDLTAATLGRMAREGDPLVDSWRAEDIPLHGARLIALLRELR"
     gene            5219..5623
                     /locus_tag="PLK64_09605"
     CDS             5219..5623
                     /locus_tag="PLK64_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80045.1"
                     /translation="MSPRNSGRIVLCTPADCRVRRDQARAFIEVAELVLTEGRREAHV
                     AAALAVLGGIAAADAVCGFALGQWSRGQDHTQAVALLGDVTLRDATLPTKLRRLLADK
                     DAAHYSPNLITVEKAKAMVRQAAALLTEADLS"
     gene            5770..6669
                     /locus_tag="PLK64_09610"
     CDS             5770..6669
                     /locus_tag="PLK64_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80046.1"
                     /translation="MSDPVVEPIEFTLNGRLYVLARAVVEGRLEGAEPRAIREHAVLV
                     NGEWFPARQALAVALGIPDHLVKSRTARAKLAALGFESRRASGAKVSDSGSDQASDSG
                     SDSATDQALGADSASEPGAALESPHRSGSRAQKPQGVEGARPPASGRPARQARRTPDP
                     GRPETDVQASVVAALAAAGWAISSVANTATKEHGIDVIAEREGQTIGVEVKGFPGLGY
                     ADPARADQRKRTRPSSQAVHWYSQAILAAMRLRTKHPEWRSVVALPDHPRYRTLHSET
                     AGSLAAAAIDVWWVDTAGDVSGL"
     gene            7146..7322
                     /locus_tag="PLK64_09615"
     CDS             7146..7322
                     /locus_tag="PLK64_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80047.1"
                     /translation="MAAIVAGDLAAVNPLIDALRDHINREENGLFPASAIALSGSEWD
                     EVERTTPPSAVHRA"
     gene            7315..>7671
                     /locus_tag="PLK64_09620"
     CDS             7315..>7671
                     /locus_tag="PLK64_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80048.1"
                     /translation="MPDAAREAAPDFTARRSVRTALLATAGVSMLAGLYAALLLVVDR
                     EPTAAQRLPDVHGILMVLGFLGTLVALERSVALRQRWAYAAPLALGLGGLAIVVPIPI
                     VVGRLLLIVGCALAMAV"
BASE COUNT         1157 a         2461 c         2727 g         1326 t
ORIGIN      
        1 gacccggcga gtgcgccgca gccgcagatc ctcgctctcg gtgcggtttt cgcggcgatg
       61 gccctggtcg gcgactcggt gtgggcgctg gtggcggggc gggcgcgaga ctggttcgcg
      121 cgcagcccgc ggcggctcga gcgcttgggc gccgggggag gcgcgctgat gatcgggctg
      181 gggctgtcag tcgcggtcgc cggccgcccg accacctgac gtcgcggcgg gcgatgggtg
      241 ggcggcatac cagaggcggg gtgacgaccc gcgcgggccg gtaggctttc cgcggcctcg
      301 ccgcacacca ctcggtatgc cgcgaccgcc cgccggcgtg gcgcaattgg cagcgcacct
      361 gtcttgtaaa cagggggtta ggggttcaag tcccctcgtc ggctccaatg tgatgtctca
      421 agacatcccg gacagggcga accctcaggg ttcgccctgt ctcgttgtct ggggttggta
      481 gcggtgggtg gtgtcgaggg tgaggtggcg caggatttcg ccggtggtgg cgtcgctgat
      541 ggtgatgttg aggccgtgga tgagggcttt gacgggtttc ccgttgtggg agcggccgat
      601 gccgatggcg tagagggtgc cgccgtggcg cagggtgatc ttgccgttgt tgaccttgtc
      661 ggtccgcacc cgggtgtggg ttcggtctgg gggttcggct gcgggggtgg ctttgggtcg
      721 ggcggtgtag gccgcggcgg gggtgcgtcg gtccagggcg cggtggggcc ggtggtggtt
      781 gtactggctc cggaaggtgt cgaggagggc ttgcaggtcg gtcagggtcg ctggttgggt
      841 gggctgccgg gcgagccagg tcttcagggt ctgttggaag cgttcgactt tgccttgggt
      901 ttgggggtgg ttgccgcggc cgttcttctg ggtgatgccg agggtggcga gttcggtctc
      961 gaaggcgttg cggccgccgc gtactcccca ggcggagtgg cggacggtat acaccatccc
     1021 gttgtcggtc agagtcgctg cggggcagcc gtattcgttg atggtggcgc ggaaggtggc
     1081 caggacgacc gggccggtga ctcgtgggtg ggcggagatg tgtagcgcca tccgggagtg
     1141 atcgtctagc caggtgatga cctcgacctc gacgccgttg gcgagacggt agtgggtgaa
     1201 gtcggactgc cagcattcgt tgggctgttc ggcggcgaac cggtggtagg cggtcttcgg
     1261 tttcttcttc ggggccggga cgacccggcc gtgggtggtc aggatccggg cgatcgatgc
     1321 ccgcgagggt ggggtcatcc cggattggtt gaggtgccag tggatggtct cagggccggc
     1381 gtcatgccca ccggcgacga gccggtcgcg ttcggccaac accgccgcca cggcctgctc
     1441 gctgaccgcg gccgggcagc tgtgcggtcg gcgggaccgg ggctcgaacg cggcttcccc
     1501 ctcgacccgc caccgggcta gcagttcgta cacccacgac cgcgccacgc cgtagctcgc
     1561 ggcgacctcg cggaccggac ggtgttgttc gacaacggca gtgatcacca gacgggcttt
     1621 cgacacccac cccagcgttc acccacgatg tccgggatgt cttgagacac ctgtccggga
     1681 tgtcctgaga ctcaacaccc cccttgcgca cgtaagggta gttccctgaa ctacccgtct
     1741 tggtttcccg aaactcccgg ccggagcatg atccggccgg gagttttcgt cgttctgggg
     1801 gtcgcgaggc ttgcgaggtg gcagcaagcc cccgggctgg ttcgtctttg gcgcaggcgg
     1861 gggtgggatc gtgatagttc ggagagactc catagttcgc tggatgagcg tagattcagg
     1921 aaatgccgta gtattagcgc gtggacacgg tgcaactcga gaggctgatg gctgacgccc
     1981 tgggtgcccg agtcgacgtg cgcgtccaag aagttgcaga ccggcgaggt cgattcacgg
     2041 ccacgatcca agcggtactc gtcgaagtcg agtggctcgg cgtcgggtgg ccacggcagg
     2101 tgcgcgagtt gctggaccgg cctgatcgtc ccgacctcgt cgtcgctccc gagttctccc
     2161 cgggagctca caagctcctg aaccaggctc ggatgtcgtg gatcgacctg acaggggccg
     2221 ccaacatcgg tctgccgggc ttgctcatca tcgtggagtc caagcgcggc gcacgaatgc
     2281 cgcgcgccag cttgggttgg acagcagggg cgctttctgt gtgtgagggg ctgctggtcg
     2341 gtgcgggagg cacggtggcg gaacttgtcg atgccaccgg gcttgccccc tcgacggtcg
     2401 cgagtgcgct caagttcctc caaggcgagc acatgctctc tgccgaggcg gcgagggggc
     2461 gaaatgcgcg acgaacgatc gtcgacagag accagattct cgatgcctat gcggctgcgg
     2521 cggcgcgact gcggagcccg gcgtcgatcc gcgtaggcgt cctctggcgg agtccagccc
     2581 gaggggctac tgaactgggc agtcgcctcg aagccaatgg tgtggcttgg gccacgacgg
     2641 gagccctcgc ggcggacgtg ctggccccgt acctcaccga ggtggacccg tgggagatct
     2701 atgtcgatgg tggctccctc gcagaactgc ggcacatcgc tcgtgaggtc gggttggcgg
     2761 aggcgagtgg aggccggttg ctgctgcggc catttcccac tcctgcgaag gacgcgctca
     2821 gctccgaagt tggtggccat cgcgtggccg cttggccacg cgtgttctcc gatctccgga
     2881 tgatcggagt tcggggcgag gaagctgccg agcatcttcg cgaacggatg ggaataggtg
     2941 aatgagcgag gacctggaac gaactagccg cgcaaggcgc gccgcggagc tcgccttggt
     3001 tcgtgtgtgc agccactatg gccagcgacc caacttcgtc ctgctcggtg gtctcgtgcc
     3061 agccatgctg tgctctgaca gtggacgtct ccacgccggg acgacggacg ttgacgtcca
     3121 ggttgacctt gagattgctg gaggtgctga gaacgcccgg cgtctggaga tcgccctgaa
     3181 gaacgccgac tttcacgtcg actccgagag ggtctggcgt tgggagacgc gccaccgatg
     3241 cgcggctcgt cgtcgctgcc ttcaccggaa ccatgctgaa cgcgattgcc ggttgacctg
     3301 gcggggcgcc tttcaggtgc aaacctatgt gctaccgacc ctgcatcgag ggcgaggtgg
     3361 cggccgatgc ccccatgccg acggggagac cgctgggtgg acagggacta ggcctcgccc
     3421 tgcagacgca gttcagggag actcgccacc atggcgttct tccgcagatc caagggtgcg
     3481 cccacggcgg ccccgccgca gcatccagtc agttccttct ggcactggtg ggccgcggag
     3541 ggtcaccaga tcgaccccac caagccctcg aaagccaccg acgcgctcag cgagagactg
     3601 ggggtgatcc accctgacct gacgtggcag ttcggtgctg gtgagggctc acggcactgc
     3661 ctgacggtct cgtcaggcgg cgtcgcccag gtgcggcccc tcgctgagcg ctggctacgt
     3721 gcagcgcctc gccccgacga gacgtgggag ttccggtcca gccagcagcg cgaccccaat
     3781 gccctcacca acgttctcga aatcgctggc caccgactcg atttgtcaga cacacgtttt
     3841 cacgtcgagt ccgatgccga cgacctgcgt attcacgtgg gagtgcacca cccggcatac
     3901 gccagcctcg cggaggaggt gcgtgggcag gtgaccttcc tgctgctcga ctggctcctc
     3961 ggcgaggacg acgtcgagcg gtgggtcggt cacgtcgagt ccctcactga ggcccccgcg
     4021 ggtctggtga ttgggcgcga ggttgtcgct gccgtgagcg acattgcggc gcaacgagaa
     4081 cccgacgaat ggaccatggg gcagtgggaa gaccgggacg gggcgccggg gcttgcgatg
     4141 ttccgcaggg gtgtccggtg gatcgaccat ccgatgctgg accggcatca ggtgttgtcg
     4201 gcggggtacg cggcgcagga caacggactg ccggcggact cgacggtgct ggatcagctg
     4261 aggcatgtcg agagcgagct tgagtcggtg ctcgggtcgc gcggactcat cgtcgccatc
     4321 gagtcacacc gcggggtgcg gacatggcat gcgtatacgg atggcgacga ccagaacgta
     4381 gacgccgctc ttgccgaggc tgcaccccga tggggcgcca cgcttgcggc ccgcccggac
     4441 cctgcctgga cggaggtccg gcagttcacc gggtgaagac ggccctcgcc atcaacgacg
     4501 agagcatcgt cgtctttgac cacttcctcg cgcgcgcagt gggatcgggt ccaactatag
     4561 ttgacccagg ccaataatag ttggatcatg aggacatgga cctttcgttt cccctcgcga
     4621 ccatcgcacc gactctggat gccggggtgt tgcatgtgct cgcggcaacg acgagcggct
     4681 gcactggggc cgaagtgcat cgtcgcctcg gtcacggcag tgacgaaggg gtccgcaagg
     4741 tcctggctcg cctcgtcggg cagggcatcg tggtcgttga aactccggcc cgctatccgc
     4801 tctatcgcct gaaccggtct cacgtagctg ccccccacat cgaggcgttg acgagggtgc
     4861 gacaggacat cgtggatcgg attcgcgatc aggtgaccgc ttggcaggtt ctgcccactc
     4921 acgccggcct gttcgggtcc ttcgctcgag gcaccgcaca cgccaacagc gacatcgacg
     4981 tgctcctcgt ccggcccgag acattgacgg agcgtgagga agacctgtgg ttggaccagg
     5041 tcgacggact cgatcgcgcc atcaaggcat ggaccggcaa cgtggcccaa atcattgacc
     5101 tcacagcagc cacgctcggg cggatggcgc gcgaaggcga tccgttggtc gactcgtggc
     5161 gggccgaaga catccccttg catggcgcgc gcctcatcgc gctgctgcgg gagctgcgat
     5221 gagcccccgc aactctggtc ggatcgtcct ctgcacgccc gccgactgtc gcgttcgtcg
     5281 ggaccaagcg cgtgcgttca tcgaggtcgc cgagctcgtg cttaccgagg gtcgccgcga
     5341 ggcccacgtc gccgcagccc tggctgtgct gggcggaatc gcggcagccg atgcggtctg
     5401 cgggttcgcg ctcggccagt ggagtcgggg ccaggaccac acccaggcag tcgccctcct
     5461 cggcgacgtc acgctgcggg acgccacgct ccccaccaag ctcaggcggc tgcttgccga
     5521 caaggacgcc gcgcattact ccccgaacct catcaccgtc gagaaggcga aggcaatggt
     5581 ccgccaggcg gctgccctct tgaccgaggc ggacctgagc tagtgccgga gcctgacgcg
     5641 acgtcattgt gtggctggga ctgcctgtga gggcgctgcg gccggacggt ggtcgccgcg
     5701 gcgggccgga ggcctgcggt tgcatgatgg acaccgtccg agcgcacacg aagccgagga
     5761 gaccgtcaca tgagcgatcc agtggtcgaa ccgattgagt tcacgctcaa cgggaggctc
     5821 tacgtgctgg cgcgggccgt tgtcgaaggc cggctggaag gtgccgagcc gagagcgatc
     5881 cgcgagcacg cggttctcgt gaacggggag tggttcccgg cccgacaggc cttggcggtc
     5941 gcgctcggga tccctgacca cctggtcaag tcgcgcactg cccgagccaa gttggctgcc
     6001 ctggggtttg agtctcgccg agcgtccgga gccaaggtct cggattcggg ctcagaccag
     6061 gcctcggact cagggtccga ttcggccacg gaccaggccc tgggcgcaga ttcagcctcg
     6121 gagccaggcg cggctttgga atcgccgcat cgcagcggct ctcgcgccca gaagccccaa
     6181 ggcgtggaag gcgcccgacc tccggcgtcg gggcgaccgg cccggcaggc gaggcgcacc
     6241 ccagatccgg ggcgtcctga gaccgacgtc caggcgtcgg tcgtggccgc gctcgccgcg
     6301 gcaggttggg ccatcagctc ggtggccaac acggcgacta aggagcacgg catcgacgtc
     6361 atcgccgagc gcgaaggcca gacgatcggc gtcgaggtca aagggttccc gggactgggt
     6421 tacgccgacc ccgcccgggc cgaccagcgc aaacgcactc gcccgagcag tcaggccgtc
     6481 cattggtatt cgcaagccat cctcgccgcg atgcggctgc ggaccaagca ccccgaatgg
     6541 cgcagtgtgg tcgccctccc ggaccacccg cgctaccgga cgctgcactc agaaaccgct
     6601 ggctcgctcg cagccgccgc catcgacgtc tggtgggtgg acaccgcagg agacgtcagc
     6661 ggcctctgac ggacgcacgg ctcgggatcg tgtggctggg acgtcctggg tggcccatcc
     6721 agccacacaa tcccggtccg agggccagtg tccggacggc aggagctaaa tagtaggctt
     6781 agaaccgtgg aaaatcagaa gtctctccta tgaccgtcca cgcgcggcgg atcccaacgc
     6841 acacgctgct cgcccaaccg gccgcccgct gatgtgctcc tactgcggct gcgagtcgat
     6901 caccacgatc ggcgacttca tggccgagca catcgagatc gtcaacgcca cgaccgctct
     6961 ccgtctggcg agcgccagcc acgacaccgc ggccgcgccg ccagctgccg cagtcctcgc
     7021 ccaaatgctc accacgcacg ctcaccgtga agaggtcggc ctcttccggg tgctcgcgcg
     7081 acaggaggag ttcacctccg aggtcaccac tctctgcggc gaacactggc tctctcgacg
     7141 cccaattggc ggccatcgtc gccggcgacc tggccgcggt caaccccctc atcgacgcct
     7201 tgcgcgacca catcaaccgc gaggagaacg gcctcttccc cgcgtcagcg attgccctct
     7261 cgggcagcga gtgggatgaa gtggagagga ccacgccgcc ctcggcggtg caccgtgcct
     7321 gacgccgccc gggaggcagc ccccgacttc accgcgcgtc gcagcgtgcg cactgcgctc
     7381 ctcgccaccg cgggggtcag catgctcgct ggcctgtatg ccgcgctcct cctcgtcgtc
     7441 gaccgggaac cgactgcggc gcaacgactt ccggacgtgc acggcatact catggtgctg
     7501 ggtttccttg gcaccctcgt tgcgctcgaa cggtccgtcg cgctgcggca acgctgggcg
     7561 tatgccgccc cgctcgccct cggtctcggc ggcctcgcca tcgtcgtccc aatcccgatc
     7621 gtggtcggtc gccttctgct gatcgtcggc tgtgccctgg cgatggcggt c
//
LOCUS       DAOMZY010000153         7661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_9027_length_7661_cov_6.678675, whole genome shotgun sequence.
ACCESSION   DAOMZY010000153 DAOMZY010000000
VERSION     DAOMZY010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7661
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9027_length_7661_cov_6.678675"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PLK64_09625"
     CDS             complement(<1..211)
                     /locus_tag="PLK64_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80049.1"
                     /translation="MSLARGGLRPAGVIAGVIAGLIANVAVRVIVGYIEVGIAVEIEV
                     AAEDWHGAGVDAQHGTLVGLGRQHGP"
     gene            251..904
                     /locus_tag="PLK64_09630"
     CDS             251..904
                     /locus_tag="PLK64_09630"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPV80050.1"
                     /translation="MSSDQPGPYPPQQGQPGYPPQTGQPGYPAPQAPPMQGAPMPGAP
                     AAYPIYPSAPPAQQWVQTGPPLASWGDRVLATLIDMLYQLPAMVLYMVGIFVAVINAP
                     TTSTRGVVVNRGNPGLMWLGLGLLAIGALGMMAISLYNVLALQGKTGQTWGKKRIGIK
                     VIHQETGVILSVGQTFLRQLCHYLDSAVYIGYLWPLWDPMRQTFADKLMKTVVIKIK"
     gene            complement(1051..1641)
                     /locus_tag="PLK64_09635"
     CDS             complement(1051..1641)
                     /locus_tag="PLK64_09635"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HPV80051.1"
                     /translation="MSTSSSRSGASAGGSNQSSGSAKPTKSAGSAARPSRSRDRIVPD
                     WLPKVALAICAIGFLVSIYLTIEAFTTGVTLACPEGETINCAKVTESEWSKLFGIPVA
                     PLGLAFFAGMAWLCRPSMFRRPGVRWDRLRLAGVGVGLAMVLYLVWAELFKIHAICLW
                     CTAVHVATFALFVALLFGQILIEPAPARVRYKDRPR"
     gene            complement(1638..2525)
                     /locus_tag="PLK64_09640"
     CDS             complement(1638..2525)
                     /locus_tag="PLK64_09640"
                     /inference="COORDINATES: protein motif:HMM:NF017831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF929 family protein"
                     /protein_id="HPV80052.1"
                     /translation="MGKSARAQNARLVELRAQQAKAERRSKLMIAGATVGVVAIVVIA
                     LVIAALQGGTDVTGQSSTALDQATFAKVTSVPAASLDTVGAGASDNPPKTIDAPALTK
                     DGKPRVLYVGAEYCPYCATERWPLVVALSRFGTWNNLSASFSAPAPEVNPNTATVSFH
                     GATYSSQYVSFTGYETSTNKQTNGQWGKLDTLEGEDLALFQKFNAPPYVQSSGAIPWI
                     NLGGTSIQAGASYNSAVILNKTQAEIATALADPTTDIAKGVNGAANVLTAQICKSTNQ
                     QPTAVCTAPGVVAAAAKLQ"
     gene            complement(2643..3767)
                     /gene="rlmB"
                     /locus_tag="PLK64_09645"
     CDS             complement(2643..3767)
                     /gene="rlmB"
                     /locus_tag="PLK64_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPV80053.1"
                     /translation="MAGNSQRRGAVRKSGKGPTVGSGGQRRRGLEGKGPTPKAADREK
                     HIAHKRSQRPADSGQGAGFGGSSSGGSGGSGSGGRYAARPASGGRPSSGGRPGSSGGR
                     SAGSGRPGSSRPTNSGRRGKPSSEIVAGRNSVVEALRADIPAATLYIASRIDSDDRVR
                     EALTLATRRGIPMLECPRAELDRITDGAVHQGLALQVPPYTYADPDDLIDPQTPGVPL
                     VVALDGITDPRNLGAILRSVAAFGGHGVVVPERRSVGMTASAWKTSAGAAARVPVALA
                     GNLTRALERFQKAGFVVVGLDMDGDVTLPEIDLGDDPVVIVVGSEGKGLSRLVRETCD
                     RVVSIPMAGPVESLNAGIAASVTLYEIARQRANVAARRVR"
     gene            complement(3769..5235)
                     /gene="cysS"
                     /locus_tag="PLK64_09650"
     CDS             complement(3769..5235)
                     /gene="cysS"
                     /locus_tag="PLK64_09650"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPV80054.1"
                     /translation="MSLHLFDTATRAMREFRPLEDGKVGIYICGLTTQGAPHIGHVRF
                     AVAFDVLRRWLTIGHGLDVTLVRNVTDIDDKILAKATEAGQEWWAWSYRNERATSEAL
                     DLLGVLPATYEPRATGHILEMVELMDTLVEKGHAYAAADGSGDVYFDVKSWPEYGSLT
                     GQALDDMAPAEDADPRGKHDPRDFALWKGHKSGEPDSAQWPTHFGAGRPGWHLECSAM
                     ARRYLGDAFDIHGGGIDLRFPHHENEQAQSRAAGLPFANFWLHNAWVTVRNQKMGKSL
                     GNALEVQVVTQVARPLAVRYYLTAAHYRSMIEYHEGSLAEAGAAVDRIEGLLERVDQV
                     RPLADRVGVLPPAFREAMDDDLNVSGALAVVHDTVRAANTALDDGDLDGAAEFAAAVV
                     AMTDVLGVNPLDPHWAGATTSGQGDATSALDALVQAQLAARQSARAARDFAAADAIRD
                     QLTTAGIAIEDTPSGPRWSLARHPDAAPGAPSVPQPKD"
     gene            complement(5419..6093)
                     /locus_tag="PLK64_09655"
     CDS             complement(5419..6093)
                     /locus_tag="PLK64_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPV80055.1"
                     /translation="MPPTLADVVAALPRSAAGVRVEHLVTSGTFSLDGGTWDVDNNVW
                     ILGDDTQCVVIDAPHDAGPILAAIGDRRLLAVLCTHAHDDHVTAALEVCGATGATAYL
                     HPADRVLWDRVHASAPDHDLADGDVLQVGDVEVQVLHTPGHAPGACCFLVPALGVLFS
                     GDTLFNGGPGATGRSFSDFPTIVESIEQRLLSLPADTVVLTGHGDATTIGAEQPHLGE
                     WLARGH"
     gene            complement(6099..7199)
                     /locus_tag="PLK64_09660"
     CDS             complement(6099..7199)
                     /locus_tag="PLK64_09660"
                     /EC_number="1.1.1.306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050607 - mycothiol-dependent formaldehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010127 - mycothiol-dependent
                     detoxification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)mycothiol dehydrogenase"
                     /protein_id="HPV80056.1"
                     /translation="MPQTVKGVIARSKGAPVELVDVVVPDPGPGEAVVVVQACGVCHT
                     DLHYREGGINDEFPFLLGHEAAGVVEAVGPGVTDVAPGDFVILNWRAVCGNCRACLKG
                     KPWYCFATHNATQKMTLTDGTVLSPALGIGAFIEKTLVAAGQCTKVSEADAGAVGLLG
                     CGVMAGFGAAVNTGGVHRGDSVAVIGCGGVGNAAIAGAKLVGATTIIAVDVDERKLEG
                     ARHFGATHTINSRERDAVEAIRELTGGHGADVVVEAVGRPETYEQAFYARDLAGTVVL
                     VGVPTPDKQVTLPMIEIFGRGGALKSSWYGDCLPSRDFPMLVELYRQGRFPLEDFVSE
                     RIGLGDVEAAFEKMHHGEVLRSVVEIAGGPSA"
     gene            7361..>7661
                     /locus_tag="PLK64_09665"
     CDS             7361..>7661
                     /locus_tag="PLK64_09665"
                     /inference="COORDINATES: protein motif:HMM:NF024988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HPV80057.1"
                     /translation="MPYPLFDELIHERTRLQLCGLLAAVVEAEFATLRDSLGASDSAL
                     SKHLKALEEAGYLTTRRQREGARSFTLVAMSDDGRHAFCGHVAAIQQLAGVASMRG"
BASE COUNT         1200 a         2620 c         2687 g         1154 t
ORIGIN      
        1 gcggaccgtg ctgccgacca agcccgacga gagtgccgtg ctgggcatcg acacccgcgc
       61 catgccagtc ctcggcggcg acctcgatct cgacggcgat tccgacctcg atatagccga
      121 cgatgacacg aacggcgacg ttggcgatga gccccgcgat gactccagcg atgacccccg
      181 cggggcgaag acctcccctc gctagactca agggacaacc cacccatccg tttcagttca
      241 ggggacaccc atgagttccg accagcccgg cccctacccg ccccagcagg ggcagcccgg
      301 ctatccgccg cagacagggc agcccggcta cccggccccg caagccccgc cgatgcaggg
      361 tgccccgatg ccgggtgcgc ccgcggcata ccccatctat ccgagcgcac cgcccgcgca
      421 acagtgggtc cagaccggac cgccgctggc gagctggggc gaccgagtcc tggccaccct
      481 catcgacatg ctctatcagc tgccagccat ggtgctctac atggtcggca tcttcgtcgc
      541 cgtgatcaac gcgccgacga cctccacgcg cggcgtcgtg gtgaaccgcg gcaatcccgg
      601 gctcatgtgg ctcggcctcg gcctgctggc gatcggcgca ctggggatga tggcgatctc
      661 gctctacaac gtgctggccc ttcaggggaa gaccgggcag acgtggggca agaagcggat
      721 cggcatcaag gtcatccacc aggagaccgg cgtgatcctg tcggtcgggc agaccttcct
      781 gcgccagttg tgccactacc tcgacagcgc ggtctacatc ggctacctct ggccgttgtg
      841 ggacccgatg cgtcagacct ttgccgacaa gttgatgaag accgtcgtca tcaagatcaa
      901 gtgacgcgct aactggcaac gctggttccg agtgcttgac cgagtgcttg accgagtgcg
      961 tttgaggggg tgatcacccc gccaatcgca ctccgtcgag cactcagtcg cagtcagtcg
     1021 gcaaggcggc cgctcagccg gtcaccacgc tcagcgaggg cggtccttgt agcgaacccg
     1081 tgccggtgcc ggttcgatga ggatctggcc gaacagcagc gcgacgaaga gcgcgaaggt
     1141 cgccacgtga acggccgtgc accagaggca gatcgcgtgg atcttgaaca actcggccca
     1201 cacgaggtag agcaccatcg ccaagccgac accgacgcca gccaggcgca gccgatccca
     1261 gcggacgccg gggcgccgga acatcgacgg ccgacacagc caggccatcc cggcgaagaa
     1321 cgccaggccg agcggtgcca ccgggatgcc gaacagcttc gaccactcgc tctcggtgac
     1381 cttggcgcag ttgatcgtct cgccctcggg gcaggccagc gtcaccccgg tggtgaacgc
     1441 ctcgatggtg aggtagatcg acaccaggaa gccgatcgca cagatcgcca gcgccacctt
     1501 gggcagccag tcgggcacga tccggtcgcg ggaccggctg ggccgagcgg ccgacccggc
     1561 cgacttggtc ggcttggccg acccgctcga ctggtttgac ccgccggccg acgccccgga
     1621 cctggacgac gacgtgctca ctggagtttc gctgccgccg caaccacacc cggcgccgtg
     1681 cagacggccg tcggctgctg gttggtcgac ttgcagatct gggcggtgag cacgttggcc
     1741 gcgccgttga ctcccttggc gatgtcggtc gtcgggtcag ccagcgcggt agcgatctcg
     1801 gcctgggtct tgttgaggat caccgcgctg ttgtacgacg caccggcctg gatcgaggtc
     1861 ccgcccaggt tgatccacgg gatcgcgccg ctgctctgga catacggcgg ggcgttgaac
     1921 ttctggaaca gcgccaggtc ctcgccctcg agggtgtcga gcttgcccca ctggccgttg
     1981 gtctgcttgt tcgtcgaggt ctcatagccg gtgaacgaga cgtactggct ggagtacgtc
     2041 gccccgtgga acgagaccgt cgcggtgttg gggttcactt cgggggcagg cgccgagaac
     2101 gacgcgctga ggttgttcca cgtgccgaat cgcgacaagg cgacgacgag cggccaccgc
     2161 tcggtcgcgc agtaggggca gtactcggca ccgacgtaga ggacgcgggg cttgccgtcc
     2221 ttggtcaggg cgggagcgtc gatcgtcttg ggcgggttgt cgctggcccc agcgccgacg
     2281 gtgtccagcg acgcggccgg cacactcgtc accttggcga aggtcgcctg atccaaggcg
     2341 gtcgacgact gcccggtgac gtccgtccct ccctgcaagg cggcgatgac caaggcgatg
     2401 accacgatgg caaccacacc gacggtcgcg ccggcgatca tcagcttgct gcgacgctcg
     2461 gccttggcct gctgcgcacg caactccacg agacgggcgt tctgagcccg ggcgctcttc
     2521 cccacggcat actccttgct gtcctggcac cgcgacgagc ggtggccaca tgctaaccag
     2581 cggacctagg tgtcgggcac caagccgtcg tgcacctgtc ctttcggaca gtcgggctgg
     2641 ccctatcgga cgcggcgggc cgcgacgttg gcgcgctgcc gggcgatctc atagagcgtg
     2701 acactcgcgg cgatgccggc gttgagtgac tcgaccgggc cggccatggg aatcgacacg
     2761 acccgatcac acgtctcccg cacgaggcgg gacaggccct tgccctcgga tccgacgacg
     2821 atgacgaccg ggtcgtcgcc gagatcgatc tcggggagcg tgacgtcgcc gtccatgtcg
     2881 agaccgacga cgacaaaacc tgccttctgg aaacgttcca gggcgcgggt gaggttccct
     2941 gcgagcgcga ccggaacccg cgcggctgct ccggccgagg tcttccacgc gctcgcggtc
     3001 atgccgaccg accggcgctc gggcacgacg accccgtgac cgccgaacgc tgcgactgag
     3061 cgcaggatgg cgccgaggtt gcgcgggtcg gtgatgccgt cgagggccac gaccaggggc
     3121 acgccggggg tctgcgggtc gatgagatcg tcggggtcgg catacgtgta gggcggcacc
     3181 tgcagcgcga ggccctggtg gacggcgccg tcggtgatcc ggtcgagttc cgcgcgggga
     3241 cattccagca tgggtatgcc gcgtcgcgtc gccagggtga gcgcctcacg gactcggtcg
     3301 tcggagtcga tgcggctggc gatgtagagg gtcgcggccg ggatgtcggc acgcagggcc
     3361 tcgacgaccg agttgcgccc ggcgacgatc tcggagctcg gcttgccgcg gcgaccgctg
     3421 ttggtgggcc ggctgctgcc cggacgtccc gaacccgcag atcggccgcc tgaggagcca
     3481 ggccgcccac ccgaggaggg ccgaccaccg gaggcggggc gggcggcata cctgccgccg
     3541 gaaccggagc ccccggagcc cccggaactg gagcccccga aaccggcacc ctgccccgag
     3601 tcggccgggc gttggctgcg cttgtgggcg atgtgcttct cgcggtcggc ggccttgggg
     3661 gtcgggccct taccttcaag gccgcgacgg cgttggcctc ccgagcccac ggtgggcccc
     3721 ttgccgctct tgcggaccgc cccgcggcgc tgggagttgc cggccatgtc agtccttcgg
     3781 ttggggaacg gacggggcgc cgggcgccgc gtcggggtgc cgggccagcg accatcgcgg
     3841 gccggacggg gtgtcttcga tggcgatccc tgcggtggtg agctgatcgc gaatagcatc
     3901 agcggcggcg aaatcccttg ccgcccgggc tgattgacgg gcagcgagct gtgcctggac
     3961 gagtgcgtcg agcgcgctcg tcgcatcgcc ctggccggac gtcgtcgccc ccgcccaatg
     4021 cgggtcgagc gggttgacgc cgagcacgtc ggtcatggcg acgaccgccg cagcaaactc
     4081 ggcagcccca tcgaggtcac cgtcgtccag agcggtgttg gccgcccgga ccgtgtcgtg
     4141 gacgaccgcg agggcgcctg agacattgag atcgtcgtcc atcgcctcgc ggaacgcggg
     4201 cggcagcacc ccgacgcgat cggcgagcgg gcgcacctgg tcgacccgct cgagcaaacc
     4261 ctcgatccga tcgaccgcag caccggcctc ggccaacgac ccctcgtgat actcgatcat
     4321 cgagcggtag tgcgccgccg tgaggtagta gcggactgcg agggggcggg ccacctgagt
     4381 gacgacctgg acctccaagg cgttgccgag cgacttgccc atcttctggt tgcgcaccgt
     4441 cacccaggcg ttgtgcaacc agaagttggc gaacggcagt ccggccgcgc gcgattgggc
     4501 ctgctcgttc tcatggtggg ggaagcgcag gtcgatgccg ccgccgtgga tgtcgaacgc
     4561 atcgccgagg tagcgccggg ccatcgccga gcactccagg tgccagcccg gtcggccggc
     4621 gccgaagtgg gtcggccatt gggcgctgtc cggctcgccg gacttgtgac ccttccagag
     4681 ggcgaagtcg cggggatcgt gcttgccgcg cgggtcggcg tcctcggccg gggccatgtc
     4741 atccagcgct tgcccggtca gcgagccgta ttccggccag gatttgacgt cgaagtagac
     4801 gtcgccgctg ccgtcggcgg cggcatacgc atggcccttc tcgaccagcg tgtccatgag
     4861 ctcgaccatc tccaggatgt gcccggtcgc gcgcggctcg taggtcgccg ggaggacgcc
     4921 gagcaggtcg agcgcttcgc tggtggcgcg ctcgttgcgg taagaccacg cccaccactc
     4981 ctggcccgcc tcggtcgcct tggcgaggat cttgtcatcg atgtcggtga cgttgcgcac
     5041 gagcgtgaca tccagaccgt gcccgatggt gagccagcgg cgcagcacgt cgaacgccac
     5101 cgcgaagcgc acgtggccga tgtggggggc gccctgggtc gtgagcccac agatgtagat
     5161 cccgaccttg ccgtcctcca aggggcggaa ctcgcgcatg gcgcgggtgg cggtgtcgaa
     5221 caggtgcagg ctcacccggg caagtttagg gggtatgccg ttcgccccct cccgaacgcg
     5281 acgcgcaccg ctgccggtgc atcggggagc ggacggggag gctcggagat ggaggagcgg
     5341 acatggaggc ggggcgggtc accgtgcccc tcaacccggt gacccgcccc gccgtctgac
     5401 gaggtcgacg ccgatgcgtc agtgacctcg agccaaccac tcccccaggt gcggttgttc
     5461 agccccgatc gtcgtcgcgt cgccgtgccc ggtgagcacg acggtgtcgg ccggcagtga
     5521 caacagccgc tgctcgatcg actcgacgat ggtcgggaaa tcactgaacg agcggccggt
     5581 cgcgcccggg ccgccgttga agagggtgtc gccggagaag aggacaccga gcgccggcac
     5641 caagaagcag caggcccctg gcgcatgacc cggcgtgtgc aacacctgga cctcgacgtc
     5701 gccgacctgc agcacgtcgc cgtcggccaa gtcgtggtcg ggcgcgctcg cgtggacgcg
     5761 gtcccacagc acgcggtcgg caggatgcag gtatgccgtg gcgccggtcg cgccacacac
     5821 ctccagggcc gccgtgacgt ggtcatcgtg ggcgtgggtg cacaagaccg caagcagacg
     5881 ccgatcacca atcgccgcga ggatcgggcc ggcgtcgtgc ggggcgtcga tgacgacgca
     5941 ctgagtgtcg tcaccaagga tccacacgtt gttgtcgaca tcccacgtcc cgccgtcgag
     6001 gctgaaggtc cccgacgtca cgaggtgctc gacccgcacg ccggccgccg aacggggcag
     6061 ggcggcgacg acgtcggcga gggtgggcgg cataccggtc atgcggaggg gccccccgcg
     6121 atctcgacga ccgagcggag gacctcgccg tggtgcatct tctcgaaggc ggcctcgacg
     6181 tcgcccagac cgatccgctc ggagacgaag tcctccaagg ggaagcgacc ctgacggtag
     6241 agctcgacga gcatggggaa gtcccgagag ggcaggcagt cgccgtacca actggacttg
     6301 agggcgccgc cgcgaccgaa gatctcgatc atcggcaggg tgacctgctt gtcgggcgtg
     6361 ggcaccccga cgaggacgac ggtgccggcc aggtcgcggg cgtagaacgc ctgctcatag
     6421 gtctcggggc gtccgaccgc ctcgacgacc acatcggccc cgtgcccccc ggtgagctcc
     6481 cgaatcgcct cgaccgcgtc gcgctcgcgc gagttgatcg tgtgggtcgc cccgaaatgg
     6541 cgggctccct caagttttcg ctcgtcgacg tcgacggcga tgatcgtcgt cgcgccgacg
     6601 agcttggctc cggcgatggc ggcgtttccg acacctccgc agccgatcac cgcaacggag
     6661 tccccccgat ggactccgcc ggtgttgacg gcggcaccga agcccgccat cactccgcag
     6721 ccgagcagcc ccaccgcgcc cgcatcggcc tcggaaacct tggtgcactg acccgccgcg
     6781 accagcgtct tctcgatgaa ggccccgatc cccagcgccg gcgagagcac ggttccgtcg
     6841 gtcagggtca tcttctgcgt ggcgttgtgg gtggcgaagc agtaccacgg cttgcccttg
     6901 aggcaggccc ggcaattgcc gcacaccgca cgccagttga ggatgacgaa gtcacctggg
     6961 gccacgtccg tgacgcctgg gccgacggct tcgaccactc cggcggcctc gtggcccagc
     7021 aggaacggga actcgtcgtt gatgccgccc tcgcggtagt gcaggtcggt gtggcagaca
     7081 ccgcaggcct ggacgacgac gaccgcctcg cccgggcccg gatctggcac gaccacgtcg
     7141 accaactcga cgggcgcgcc cttcgagcgg gcgatgactc ccttgacggt ctgcggcata
     7201 ccgaatcctc cagggcagca cgaagtggac gtgacgtgtc cctaggagag tgggcgcggt
     7261 gtgggacgcg caagaccaag gccctcgaaa ccggcgtttg cgctggtggg aggcggtccg
     7321 cggagtttcc ttagaggcaa ccgggcctta tcgttgagcc atgccttacc ccttgttcga
     7381 cgagttgatc cacgagcgga cccgcttgca actgtgcggg ctgctcgcgg cggtcgtcga
     7441 ggccgagttc gccaccctgc gcgacagcct cggggcgagc gactccgcgc tgtccaagca
     7501 cctcaaggcg ctggaagagg ccggctatct cacgacccgt cggcagcgcg agggcgcccg
     7561 atcgttcacc cttgtcgcga tgagcgacga cgggcggcat gcgttctgcg ggcacgtggc
     7621 ggcgatccag caactggccg gggtggcgtc gatgcgcggc a
//
LOCUS       DAOMZY010000154         7580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_9150_length_7580_cov_6.204385, whole genome shotgun sequence.
ACCESSION   DAOMZY010000154 DAOMZY010000000
VERSION     DAOMZY010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7580
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9150_length_7580_cov_6.204385"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="PLK64_09670"
     CDS             <1..742
                     /locus_tag="PLK64_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPV80058.1"
                     /translation="KWATTKHKKAAIDAKRGKLFAKLIKNIEVAARTGGGDPLGNPTL
                     WDAIYKAKKSSVPNDNIDRAVKRGSGAEAGGAEYKEITYEGYAPNGVALYIQCLTDNR
                     NRAAAEVRTALTRNGGNLADPGSVAYMFTRKGVVIVPKGSHTEDTLLEVVLDVGAEEI
                     NDLGEAFEIVSEASDFVEVRKALQAAGIDYDSAEATFIPSVEVPLDLEGARKMMRVID
                     ALEDSDDVQNVYANGDISDEVLAQLDEE"
     gene            complement(787..1188)
                     /locus_tag="PLK64_09675"
     CDS             complement(787..1188)
                     /locus_tag="PLK64_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPV80059.1"
                     /translation="MSTKSTGGRQRASSGSPFEATIGFSRGLRDGEVIAIAGTAPVWP
                     DGSVDPDPLVQARRAWAIALTALSQLGGRPQDVVRTRTYLTTTEVAEVAAEAHGEIFS
                     DVRPASTMVVVAALLDPRWVIEVELDAIVRG"
     gene            1319..1870
                     /gene="ruvC"
                     /locus_tag="PLK64_09680"
     CDS             1319..1870
                     /gene="ruvC"
                     /locus_tag="PLK64_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPV80060.1"
                     /translation="MRVLGVDPGLTRCGVGVVEGDLGRRLAMVDVTVVRSDSKTPTPE
                     RLLALDLALTELVGRHRPDAIAVERVFADVNVLSVMGTAQASAVAMLVAARHGIPVYL
                     HTPTEVKAAVTGSGRAEKAQVTAMVTRLLRLPTAPTPADAADALALAICHLWRGAATN
                     RIEQAAATVRAQQAAALAGRTGR"
     gene            1867..2466
                     /gene="ruvA"
                     /locus_tag="PLK64_09685"
     CDS             1867..2466
                     /gene="ruvA"
                     /locus_tag="PLK64_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPV80061.1"
                     /translation="MIASVRGEVSHVGLDNVVVEVGGVGVLVHVTPATAATLRTGSTA
                     YLATSLVVREDSLTLYGFADADGRSVFETVQTVTGVGPRLALAMLAVHSPDGIRAAVA
                     SGDLTALMKVPGIGRKGAERLVLELRDKMGMPSGPVGSPTAAAAGPDWAHQVREALVG
                     LGWSAKQAEDTVEAITPEAGERPDVSGLLRTALQRLART"
     gene            2463..3593
                     /gene="ruvB"
                     /locus_tag="PLK64_09690"
     CDS             2463..3593
                     /gene="ruvB"
                     /locus_tag="PLK64_09690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPV80062.1"
                     /translation="MTASDDPTATDGPGGPTWADGSWEATARVVDPSGLDRERQVEAA
                     LRPRRLAEFPGQTRVRDQLALVLEAARRRGTPPDHVLLSGPPGLGKTTLAMIIAAELE
                     QPIRVTSGPAIQHAGDLASILSSLAEGEVLFLDEIHRMSRAAEEMLYLAMEDFRVDIV
                     VGKGPGATAIPLELPPFTVVGATTRSGLLPAPLRDRFGFTGHLDFYETADLVTILRRS
                     ARLLDVRAEDAGIEEIAKRSRGTPRIANRLLRRVRDWAQIHGQDVVDLDAAHEALALF
                     DVDVRGLDRLDRAVLDALCRRFGGGPVGVANLAVAVGEEADTVETVAEPFLVREGLIV
                     RTSRGRAATALAWEHLGLAPPSQAAAYQASLLTPDDVGRFGT"
     gene            3719..4033
                     /gene="yajC"
                     /locus_tag="PLK64_09695"
     CDS             3719..4033
                     /gene="yajC"
                     /locus_tag="PLK64_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPV80063.1"
                     /translation="MTSNDLSSLLFFAIPVGFIAYIFLGQRKRVRAMQAMQASIVVGE
                     EVRTTSGLFGRVTALTDSEIHLEVSPGVVVRFDRRAVDARTSGAAPSSSNGPADAPAQ
                     GE"
     gene            4045..5844
                     /gene="secD"
                     /locus_tag="PLK64_09700"
     CDS             4045..5844
                     /gene="secD"
                     /locus_tag="PLK64_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPV80064.1"
                     /translation="MALNPRTRGPRRTLWTFAAIIIALFVLIFSVNRWGTGQLSPKLG
                     LDLEGGTQMVLEPVLADPNAEVSSGQLEKARDIIAQRVDSNGVAEAEVSTQGGRNIVI
                     SIPGQPDAKTLDQIRKPSQLRFRAVLVTGSGIPATVTTPTSTATGTQTSEPTATTPKP
                     VFTMDPAPTATSPATSGNAVVPPALVSETSPTAPAATSTPTATAAATPTPANASDLAW
                     ITPEIEAEFTALDCTKAGAINDFVDDPAKPLVTCSTDRVEKYILGPVELDGTDIADAT
                     SGYQSGNNGQPTNIVEVRLNFTGEGGQKFANVTTRLYALQAADQTRNRFAIVLDKQVI
                     SAPSTNAVIANGQASITGNFTIESARSLAQQLKFGALPMSFALQTQDDISPTLGKEQL
                     GLGLLAGLIGLLLVVGYSLFQYRLLGLVTVASLIVAAVLTYGVLTLLGWSHNFRLTMA
                     GVTGVIVAIGVTADSFIVYFERVRDEVREGRPLVTAVDSGWRRARRTILAADGVNLLA
                     ATVLYLLAASNVRGFAFTLGVTTLIDLAVVFLFTHPLVSILAHTKFFGGGHPWSGLDP
                     ERLGAKTTQPRYAGAGRFTGTPVRTAAAKEAKA"
     gene            5844..7001
                     /gene="secF"
                     /locus_tag="PLK64_09705"
     CDS             5844..7001
                     /gene="secF"
                     /locus_tag="PLK64_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HPV80065.1"
                     /translation="MINFSQFGNDLYTGRRSIDFIGRQRRWYTFSGILIVLALVGMFA
                     RGLNFGIEFSGGSEFRLTGVTDTSNYEDKAKAAVSEAGMGGNVLASVIGGATVRVQTE
                     AAGEHNEAAKAALAKAFNVDQTTVSASLIGPSWGQSVSQKAVQALITFLLLVALVMAI
                     YFRTWKMAVAGLVALLHDLIITVGIYALFGFEITPASMIGFLTILGYSLYDTVVVFDK
                     VRENTADAIGSRRFSYVQAANLAVNQTLVRSINTTVVALLPIFAILMVGVTKLGPGTL
                     LDLSLALFVGIAVGAYSSIFIATPLLVDLRRGEPALQELAKHAAKVQAKDEALVTPAG
                     AKIADAEVSGRHTGVIADAPVDDLDAVGRPVHKWAATGPRNQPKRPPRSRR"
     gene            7004..7561
                     /locus_tag="PLK64_09710"
     CDS             7004..7561
                     /locus_tag="PLK64_09710"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HPV80066.1"
                     /translation="MSDEQIRQLVLDHLRDIPDFPKPGVLFKDFTPLLADGDALRAVA
                     DHLAQRYAGRVDAVVGIEARGFILGAATAYAMGVGFVPVRKAGKLPGETYEATYTLEY
                     GTATLEIHVDAFVEGQRVLIMDDVLATGGTAAATCELVERAGGQVVAVDVVLEIGGFN
                     GRDALSVDEERDDLAAEGGGRGRHG"
BASE COUNT         1151 a         2695 c         2545 g         1189 t
ORIGIN      
        1 caagtgggcg accaccaagc acaagaaggc ggcgatcgac gccaagcgcg gcaagttgtt
       61 cgccaagctc atcaagaaca tcgaggtggc ggcccgcacc ggcggcggcg accccctcgg
      121 caaccccacc ctctgggacg ccatctacaa ggcgaagaag tcgtcggtcc ccaacgacaa
      181 catcgaccgc gcggtcaagc gcggttcggg tgctgaagcc gggggagcgg agtacaagga
      241 gatcacctac gagggctacg cccccaacgg tgttgccctc tacatccagt gcctcaccga
      301 caaccgcaac cgtgccgccg ccgaggtgcg caccgctctc acgcgcaacg gcggcaacct
      361 cgccgatccc ggctcggtgg cctacatgtt cacccgcaag ggcgtcgtca ttgtccccaa
      421 gggcagccac acggaggaca cgctcctcga ggtggtgctc gacgtgggcg ccgaggagat
      481 caacgacctc ggtgaggcgt tcgagatcgt gtcggaggcc agtgacttcg tcgaggtccg
      541 caaggcgctg caggcggccg gcatcgacta cgactcggcc gaggcgacct tcatccccag
      601 cgtcgaggtg ccgctggatc ttgagggtgc gcgcaagatg atgcgggtga tcgacgcgtt
      661 ggaggactct gacgacgtgc agaacgtcta tgccaacggc gacatcagcg acgaggttct
      721 cgctcagctc gacgaggagt aaagcccttc tcggccctcc gccggcgcac ccaccgatgc
      781 cgggcactaa ccccgcacga tggcgtccaa ctcgacctcg atgacccagc gggggtccag
      841 cagcgcagcg acaacgacca tcgtcgaagc cggtcgcacg tcgctgaaga tctcgccgtg
      901 ggcctctgct gccacctcgg cgacctcggt cgtcgtcagg taggtgcggg ttcgcacgac
      961 gtcctgcggt cggcccccca gctgtgacag ggcggtgagc gcgatcgccc aggcacgtct
     1021 ggcctggacg agcgggtccg ggtcgacgct gccgtccggc cagacggggg ccgtgcccgc
     1081 gatggcgatg acctcgccgt cgcgcaatcc ccggctgaaa ccgatggttg cctcgaacgg
     1141 tgatccggat gacgcgcgct gccgcccgcc ggtcgactta gtgctcatgg cagtgattct
     1201 ccggcacgtc ggaggcctcg gcgcaccccg tcccggcgcg tcgcgaaagg agccaccggc
     1261 cccaacgggg tagcgtgact cccgaacacg tgttcgcacc agacatggga ggtatgccgt
     1321 gcgcgtcctg ggcgtcgacc cgggcctgac ccggtgcggg gtcggtgtcg tcgagggcga
     1381 cctcggccgc cgactggcca tggtcgatgt caccgtcgtg cgcagcgact ccaagacccc
     1441 gacgcccgag cgcctattgg cgctcgacct ggccctcacc gagttggttg gccggcaccg
     1501 cccggatgcc atcgccgtcg agcgggtctt cgccgacgtc aacgtcttgt cggtcatggg
     1561 gaccgcgcag gcctcggcgg tggcgatgct cgtcgcggcg cggcacggca tacccgtcta
     1621 tttgcacact ccgaccgagg tgaaggccgc ggtcaccggc agcggccgcg ccgaaaaggc
     1681 gcaggtgacg gccatggtga cccggctgct tcggctgccg accgcaccca ccccggccga
     1741 cgccgccgac gccctcgccc tggcgatctg ccacctgtgg cgcggcgcgg cgaccaaccg
     1801 gatcgagcag gccgcggcga ccgtccgggc ccagcaggct gccgcactgg caggaaggac
     1861 cggccgatga tcgcctccgt ccgtggcgag gtcagccacg tcgggttgga caacgtcgtc
     1921 gtcgaagtgg gcggcgtcgg ggtgctcgtg cacgtcacgc ccgccaccgc ggcgaccctg
     1981 cggaccggca gcacggcata cctcgcgacc tcgctcgtcg tgcgcgagga ctcgttgacg
     2041 ctctatggct tcgccgacgc ggacggccgc tcggtcttcg aaaccgtcca gacggtgacc
     2101 ggtgtggggc cgagactcgc gctggcgatg ctcgccgtcc atagccctga cggcatccgt
     2161 gcggccgtcg cctctggcga cctcaccgcg ctgatgaagg tgcccggcat cggccgcaag
     2221 ggtgccgagc ggctggtgct cgagcttcgc gacaagatgg gtatgccgtc cggcccggtc
     2281 ggcagcccga ccgccgccgc tgcgggcccc gactgggcgc accaggtccg cgaggccttg
     2341 gtcggcctcg gatggtcggc caagcaggcc gaagacaccg tcgaggcgat cacgcccgag
     2401 gccggtgagc ggccagacgt gtcggggctg ctgcgcaccg ccctgcaacg cttggcccgc
     2461 acgtgaccgc gagcgacgac cccaccgcga cggacggtcc cggcggcccg acctgggccg
     2521 acgggtcgtg ggaggcgacg gcgcgagtcg tcgaccccag cggcttggac cgtgagcgcc
     2581 aggtcgaggc cgccttgcgc ccccggcgcc tggccgagtt ccctgggcag acgcgggtgc
     2641 gcgaccagtt ggccctcgtc cttgaggccg cgcgtcgccg tggcacgccg ccggaccacg
     2701 tgctgctctc cgggcctcct ggcctgggca agaccaccct ggcgatgatc atcgcggccg
     2761 agctcgagca accgatccgg gtgacgtcgg ggccggccat ccaacacgcc ggtgacctgg
     2821 cctcgatcct ctcctcgctg gccgagggtg aggtgctctt cctcgacgag atccaccgga
     2881 tgtcccgcgc cgccgaggag atgctctatc tggcgatgga ggacttccgg gtcgacatcg
     2941 tcgtcggaaa gggtccgggg gcgacggcca tcccgttgga gttgccgccg ttcaccgtcg
     3001 tcggcgcgac gacccgctct gggctgctgc cggctccgct gcgcgaccga ttcggcttca
     3061 ccgggcactt ggacttctac gagacggccg atctggtgac gatcctgcgc cgttcggccc
     3121 ggttgctcga cgtgcgtgct gaagacgccg ggatcgagga gatcgccaag cggtcccgcg
     3181 gaacacctcg aatcgccaac cgcctgctac gccgggtgcg tgactgggcg cagatccacg
     3241 gtcaggacgt cgtcgacctt gacgcggccc acgaggcgct ggccctcttc gacgtcgacg
     3301 tccgcggctt ggaccggctc gaccgggccg tgctcgacgc gctgtgccgg cgcttcggcg
     3361 gcggtccggt cggagtggcc aaccttgccg ttgccgtggg ggaggaggcc gacaccgtcg
     3421 agacggtcgc cgaaccgttc ctcgtgcggg aagggctcat cgtccgcacg tcccgtggcc
     3481 gagccgcgac cgccctcgcg tgggagcacc tcggcctcgc cccgccgagc caggcggcgg
     3541 cataccaggc gtcgttgctg acccccgacg acgtcggtcg tttcgggacc tgaggcgtat
     3601 gccgcgtgct ttccggcgcg gctggttcgc ctggggccgg ttgataggcc agaatgggac
     3661 gctggtctgc ctgttcggca gtggcgtgct tcaccggtga taaaggtaac gacgagacat
     3721 gacgagcaac gacctgagtt cgctgctgtt ctttgcgatc cccgtgggct tcatcgccta
     3781 catcttcctc ggccagcgca agcgggtccg cgccatgcag gcgatgcagg catcgatcgt
     3841 tgtcggtgag gaggtgcgga ccacctcggg cctcttcggt cgggtcaccg cgctgaccga
     3901 ctcggagatc cacctggagg tgtcccccgg agtcgtcgtg aggttcgacc gccgcgccgt
     3961 ggacgcccgc acgtccggtg ctgcaccgtc gtcgagtaac gggccggccg acgccccggc
     4021 ccagggcgag tgagggaacc cgtcatggca ttgaaccccc ggacgcgcgg gccccgtcgc
     4081 accctgtgga ccttcgcggc gatcatcatc gccctcttcg tgctcatctt ctcggtcaac
     4141 cgctggggga ccgggcagtt gagccccaag ctgggtctgg acctcgaggg cggcacccag
     4201 atggtccttg agccggtgct cgccgacccc aatgcggagg tgagctccgg gcagctcgag
     4261 aaggcccgcg acatcatcgc ccagcgggtc gactccaacg gcgtcgccga agccgaggtg
     4321 tcaacccagg gcgggcggaa catcgtcatc tcgatccccg gtcagcccga cgccaagacg
     4381 ctcgaccaga tccgcaagcc gtcgcagttg cgcttccgcg ctgttcttgt gaccgggtcc
     4441 ggcattcccg ccaccgtcac gacgcccact tcgacggcca ccgggacgca gacgagcgag
     4501 ccgaccgcga cgacgccgaa gccggtcttc accatggacc cggcccccac ggcgacgagc
     4561 ccggccacga gtggcaacgc cgtcgttccg ccggcgttgg tctccgagac gtccccgacg
     4621 gcgccggcag ccacgtcaac gcccacggcc actgcggcgg ccaccccgac gccggccaac
     4681 gcctccgacc tggcctggat cacgcccgag atcgaggcgg agttcacggc cctcgactgc
     4741 accaaggccg gcgcgatcaa cgacttcgtc gacgaccccg ccaagccgct ggtcacctgc
     4801 tcgacggacc gggtcgagaa gtacatcctc ggtcccgttg agctcgacgg caccgacatc
     4861 gccgacgcga cgtcgggcta ccagtcgggc aacaacggcc agccgaccaa catcgtcgag
     4921 gttcggctga acttcaccgg tgagggcggc cagaagttcg ccaacgtgac cacccggctc
     4981 tacgcgctgc aggccgccga ccagacccgc aaccggttcg ccatcgtcct ggacaagcag
     5041 gtcatctcgg cgccgtccac caacgccgtc atcgccaacg gccaggcctc gatcaccggg
     5101 aacttcacga tcgagtcggc ccgctcgctg gcccagcagt tgaagttcgg tgcgctgccc
     5161 atgtcgttcg ccctgcagac gcaggacgac atcagcccca ccctcggcaa ggagcagctc
     5221 gggttgggcc tgctggccgg cctcatcggc ctgctgctcg tcgttggcta ttcgctcttc
     5281 cagtaccgac tgcttggttt ggtcacggtg gcctcgctga tcgtggcggc cgtgctcacc
     5341 tacggggtgc tcaccctgct gggttggtcg cacaacttcc gcctgacgat ggccggcgtg
     5401 accggtgtca tcgtcgccat cggtgttacc gcagactcgt tcatcgtcta cttcgagcga
     5461 gtccgtgacg aggttcgtga gggacgaccc ttggtcacgg ccgtcgactc gggttggcgg
     5521 cgggctcggc gcacgatttt ggcggccgac ggcgtcaacc tgctcgcggc caccgtgctc
     5581 tacctgctcg cggccagcaa cgtccgcggc ttcgccttca cgctgggcgt caccaccctc
     5641 atcgacctcg ccgtggtgtt cctcttcacc cacccgctgg tgtcgatcct ggcgcacacc
     5701 aagttcttcg gtggcggaca cccgtggtcc ggccttgacc ccgagcgact cggcgccaag
     5761 acgacccagc cgaggtatgc cggtgccggt cgtttcaccg gcaccccggt ccgcaccgct
     5821 gcggctaagg aggcgaaggc ctgatgatca acttctcgca gttcggcaac gacctctaca
     5881 ccggccgtcg gtcgatcgac ttcatcggcc gccaacgtcg ttggtacacc ttctcgggca
     5941 tcctcatcgt gctggccctc gtcggcatgt tcgctcgcgg cctgaacttc ggcatcgagt
     6001 tcagtggcgg ttcggagttc cggctgaccg gagtcaccga caccagcaac tacgaggaca
     6061 aggccaaggc ggccgtcagc gaggccggga tgggcggcaa cgtcctggcc agcgtcatcg
     6121 gcggagcgac cgtgcgggtc cagaccgagg ccgccggcga gcacaacgaa gccgccaagg
     6181 ccgccctggc caaggcgttc aacgttgacc agaccactgt ctcggcatcg ctcatcgggc
     6241 cgtcctgggg acagtcggtg agccagaagg ccgtccaagc cctcatcacc ttcttgctgc
     6301 tggtcgcgct cgtcatggcg atctacttcc gcacctggaa gatggccgtg gcgggcctgg
     6361 tcgccctgct ccacgacctc atcatcacgg tcgggatcta tgccctcttc ggcttcgaga
     6421 tcaccccggc gtcgatgatc ggcttcctca ccatcctcgg ttactccctt tatgacaccg
     6481 tcgtggtctt cgacaaggtg cgggagaaca ccgccgacgc gatcggcagc cgccggttct
     6541 cctacgtcca ggcagccaac ctcgcggtca accagaccct cgtccggtcg atcaacacga
     6601 cggtcgtcgc cctgctgccg atcttcgcca tcctcatggt cggcgtgacc aaactcggcc
     6661 caggcacgct gcttgacctg tccctggcgc tgttcgtcgg tatcgccgtc ggtgcctact
     6721 cgtcgatctt catcgccacg ccgctgctgg tcgacctgcg gcgcggcgag cccgccctgc
     6781 aggagttggc caagcacgcg gccaaggtcc aggccaagga cgaggctctg gtgacccccg
     6841 cgggcgccaa gatcgccgac gccgaggtga gcgggcggca taccggagtc atcgccgacg
     6901 cgcccgtcga cgacctcgac gcggttggcc gacccgtgca caagtgggcg gccaccgggc
     6961 cacgcaacca acccaagcgc ccgccgcggt cgcgccgctg acgatgagcg acgagcagat
     7021 tcgccagttg gtcctcgacc acctgcggga catcccggac ttcccgaagc cgggggtgtt
     7081 gttcaaggac ttcaccccgc tgctcgccga cggcgacgcg ctgcgggcgg tcgctgatca
     7141 cctcgcgcag cggtatgccg gtcgcgtgga cgccgtcgtc gggatcgagg cgcgggggtt
     7201 catcctcggc gccgcgacgg catacgcgat gggtgtgggg ttcgtgcccg tgcgcaaggc
     7261 ggggaagctg ccgggggaga cctacgaagc gacctacacg ctggagtacg gcaccgcgac
     7321 cctggagatc cacgtcgatg cgttcgtcga gggtcagcgg gtgttgatca tggacgacgt
     7381 gctggcgacc ggcggcacgg ctgcggccac gtgtgagctc gtggagcggg ccggtggcca
     7441 ggtcgtcgcc gtcgatgtcg tcctcgagat cggcggattc aacgggcggg acgccctgtc
     7501 agtcgacgag gagagagacg acctcgcggc ggagggcggc gggagaggtc gtcatgggtg
     7561 actacacttc ggacatgacc
//
LOCUS       DAOMZY010000155         7539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_9224_length_7539_cov_6.295831, whole genome shotgun sequence.
ACCESSION   DAOMZY010000155 DAOMZY010000000
VERSION     DAOMZY010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7539
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9224_length_7539_cov_6.295831"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PLK64_09715"
     CDS             <1..288
                     /locus_tag="PLK64_09715"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPV80067.1"
                     /translation="PRFTWWANVVTDAGHHAAGPRSEPAREALRQADARLGVFLDELA
                     ARGELDDVAIVLTADHGFEGSDPACTGSWRSALDALGIPYRDEGPGFVYLV"
     gene            complement(276..947)
                     /locus_tag="PLK64_09720"
     CDS             complement(276..947)
                     /locus_tag="PLK64_09720"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HPV80068.1"
                     /translation="MCEPLPGALPDTLPDALPDTLDIALVGVGGQGTILASNVLAELG
                     MAAGYDVKKAEVHGMSQRGGSVVSHVRWGPEVFSPIIPAGGADILVAFEKMEAARYAA
                     LLAPGGTAIVNDFAIEPITVITGGAHYPDDAAIEAAFADGGQRLMWIHGARVAEELGN
                     KFVANVVLLGALSTLLGLPEAAWAAVLDRRVKAAHLEINHRAFAAGRAEMSAPAPTRP
                     QPQTR"
     gene            complement(940..2820)
                     /locus_tag="PLK64_09725"
     CDS             complement(940..2820)
                     /locus_tag="PLK64_09725"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPV80069.1"
                     /translation="MKRLLSGNEAIARAAWEAGAVLGAGYPGTPSTEILEAFAPYPDV
                     YAEWSPNEKVALDVAIGSAIAGRRAFATMKHVGLNVALDSFWSASMTGIEAGLVVVSA
                     DDPSLHSSQNEQDNRFLARWARVPCLDPADSQDSHDFMVAAFDLSERFDTPVLLRPTT
                     RVCHTSSPVEVGEIRLRRPVPDTYVRNLAKYDMAPANARARRPVIEHRVEALADFAET
                     FPLNRVEMRRTTIGIVTSGVAYAYAREVFPDASTLKLGMVYPLPRRLIADFAAQVDSL
                     IVLEELDPFVEDQLRLMGIRLHTPPLPPTYPHTKSIFPLMGELDPAVVRSSAVAAGLL
                     DASVASPVAASGIPYAAGSPAPASRTLSLTPVDVAPRPPVLCPGCPHRSSFYVLHKLG
                     VTVNGDIGCYTLGMAKPLDALHTAGCMGASIAVAHGGAQAGDQGRHVAVIGDSTFFHS
                     GIPALLNVLYNRSNVVTIIMDNRITGMTGHQDNPGSGHTLQRQETVEVDIERVVRALG
                     FPDDKVRTIPSYDVKALEKQLKTWLKEDGPAVLITQEECALLPTAKAAYLPLAVTDAC
                     NGCTICFRIGCPAILQSDVLDERTQRPLALIDQVMCTGCEICAQVCPRDAILFREQVA
                     HV"
     gene            3107..3634
                     /locus_tag="PLK64_09730"
     CDS             3107..3634
                     /locus_tag="PLK64_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80070.1"
                     /translation="MGLVGLVIPASASAMPYVDSTPSASSLPAGLQPLPDEDLNASSK
                     RLSLAACNALVQKGIIYHPAGWKFSCSSSRVGKSYNAWTFRRETVVKPGMTLAKTKAV
                     LAHEEAHIWSLSVLTPAQMTWFSRQLGKRSFFDGTTRTMPAEVWAASQAACAGYPQGP
                     YKRVSCSLLRETLRH"
     gene            4681..5340
                     /locus_tag="PLK64_09735"
     CDS             4681..5340
                     /locus_tag="PLK64_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80071.1"
                     /translation="MGPPAEHVFDVCDSQNAGEYLLAGSSGGFSAVVWRTTDYGRTWA
                     ELWREPEESASMDGYERFYWLTRIGNRIFMRADPGTKGTKPPMRYYDTRRQRMNKVPN
                     SAVTVGGSKFYPVILGDRVQRANQVCGDGRIAYFITDGKVFAFDGAQSSQVSSVWGVL
                     SPGDDGRIYLKDATGVYVLTGTTKTTILAQADLPGVSMTVANGRIWLGGSQSEIWSRP
                     L"
     gene            5943..>7539
                     /locus_tag="PLK64_09740"
     CDS             5943..>7539
                     /locus_tag="PLK64_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPV80072.1"
                     /translation="MAAVAVAAVATASLGIIYATGVAAVATATQQAHVGSIGWMTPVQ
                     PGSVLGQPGQNRAIEAIKLTELPTGSTISAHVSSIGWMAPVGPGMVAGTTGRGLQMEA
                     VKVTVPSGCTVTYQAYVRGQGWSASVSNGAIAGTTGKALALDGLRVNAVTCATSTTST
                     TTPPVAGTQHAFFGGAWAPAVRVGEVIGQPGQNRAIEAIKLTELPTGSTISAHVSSIG
                     WMAPVGPGMVAGTTGRGLQMEAVKVTVPSGCTVTYQAYVRGQGWSASVSNGAIAGTTG
                     KALALDGLRVNAVTCATTPPPASVKFAAVGDIGTDSENGSTAASSMLKGLGANGYNFA
                     LNLGDLGYNNNPGVEQNYCTFLKNNAGSSLQWLMVPGNHEGTDPTYGGPEGDYRKYVA
                     YCAGSTPPAGVVGNYPYDYYVDRGNVRIIMLSPNISNPAGKLTYRNGTAEQATLGTWI
                     QGAKSAGKFVVVGAHEPYLTIGEHRLTTDNLADPQFAAFLVAQRVDLSLSGHDHNISR
                     AYQLGGSVTSQTSPTVVDRDGSFV"
BASE COUNT         1314 a         2381 c         2520 g         1324 t
ORIGIN      
        1 ccgagattca cctggtgggc caacgtcgtc accgacgccg ggcaccatgc cgcaggcccc
       61 cgctccgagc cggcccggga ggcactgcgc caggccgacg cgcggctcgg ggtgttcctc
      121 gacgagcttg cggcccgcgg cgagctcgac gacgtcgcga tcgtgctcac cgccgaccat
      181 ggcttcgaag gctcagaccc ggcctgcacc gggtcgtggc gatcggcgtt ggacgcgctg
      241 gggatcccct atcgcgacga agggccgggg ttcgtctacc tcgtctgagg ctgcggtcgg
      301 gtgggtgccg gggccgacat ctcggcgcgg ccggcggcga aggcccggtg gttgatctcc
      361 aggtgagccg ccttgacccg gcgatcaagg acggcggccc aggcggcctc gggaagcccg
      421 agcagggtcg acagggcacc gagcaggacg acgttggcga cgaacttgtt gcccaactcc
      481 tcagcgaccc gtgccccgtg gatccacatc agccgttgtc cgccgtcggc gaaggccgcc
      541 tcgatggccg cgtcgtcggg gtagtgggcc ccgccggtga tgaccgtgat cggctcgatg
      601 gcgaagtcgt tgacgatggc cgtgccgccg ggagccagca gcgcggcata ccgggcggcc
      661 tccatcttct cgaaagcgac gaggatgtcc gctccgccgg cggggatgat cggcgagaac
      721 acttccgggc cccaccgcac gtgcgagacg acgctgccgc cgcgctggga catgccgtgg
      781 acttcggcct tcttcacgtc gtaacccgcg gccatgccca actcggccag gacgttgctc
      841 gcgaggatcg tcccttgccc gccgacgccg acgagcgcga tatcgagagt gtcggggagc
      901 gcgtcgggga gagtgtcggg gagcgcgccc gggagcggct cacacatgcg ccacctgctc
      961 gcggaagagg atcgcgtcgc gcgggcacac ctgggcgcag atctcgcacc cggtgcacat
     1021 cacctggtcg atgagggcca gcgggcgttg ggtgcgctcg tcgaggacgt cggactgcag
     1081 gatcgccggg cagccgatcc ggaagcagat cgtgcagccg ttgcaggcgt cggtcaccgc
     1141 cagcggcagg taggccgcct tggccgtcgg caggagggcg cattcctcct gggtgatgag
     1201 caccgccggg ccgtcctcct tgagccaggt cttgagctgc ttctccaggg ctttgacgtc
     1261 ataggacggg atggtgcgga ccttgtcgtc ggggaagccg agggctcgca cgacccgctc
     1321 gatgtcgacc tcgacggtct cctgccgctg cagagtgtgg ccggagccgg ggttgtcctg
     1381 atggccggtc atgccggtga tccggttgtc catgatgatc gtcacgacgt tgctgcggtt
     1441 gtagagcacg ttgagcagcg ccgggatgcc gctgtggaag aacgtcgagt caccgatgac
     1501 ggccacgtgg cgcccttggt cgccggcctg cgcgccgccg tgcgcgacgg cgatgctcgc
     1561 gcccatgcat cctgccgtgt gcagcgcgtc gagcggcttg gccatcccca gcgtgtagca
     1621 cccgatgtcg ccattgacgg tgacgcccaa cttgtgcagg acatagaacg acgaccggtg
     1681 cgggcacccc gggcagagga ccggcgggcg gggggccacg tcgacggggg tgagcgagag
     1741 ggtgcgggag gcgggggcgg ggctgcccgc ggcatacggt atgccgctcg cggccacggg
     1801 gctggcaacg gaagcgtcga gcaggcccgc agcgacggcg ctggagcgga cgaccgccgg
     1861 gtcaagctca cccatgaggg ggaagatcga cttcgtgtgc gggtaggtcg gggggagcgg
     1921 cggcgtgtgc agccgaatgc ccatgagccg cagttggtcc tcgacgaacg ggtcgagctc
     1981 ctcgaggacg atgagcgagt ccacttgggc cgcgaagtcg gcgatgaggc ggcgggggag
     2041 cggatagacc atgccgagtt tgagggtgct tgcgtcgggg aagacctcgc gggcgtaggc
     2101 gtacgcgacg ccgctggtga cgatgccgat cgtcgtgcga cgcatttcca cccggttcaa
     2161 ggggaaggtc tcggcaaagt cggcaagagc ctcgacccgg tgttcaatca cggggcggcg
     2221 ggctcgggcg ttggccggtg ccatgtcgta cttcgcgaga ttgcgtacgt acgtgtcggg
     2281 gaccggtcga cgcagccgga tttcgccgac ctcaaccgga gaactggtgt gacacacccg
     2341 agtggtcggg cgcagcagga cgggggtgtc gaaccgctcg gacaggtcga aggcggcgac
     2401 catgaagtcg tggctgtcct ggctgtcggc agggtcgagg caggggaccc gggcccaacg
     2461 agccaggaag cggttgtcct gctcgttctg cgatgagtgc aggctggggt cgtcggccga
     2521 gacgaccacg aggccggcct cgatgccggt catcgacgcc gaccagaacg agtcaagtgc
     2581 gacgttgagg ccgacgtgct tcatcgtggc gaaggcgcgg cgaccggcga tcgcggaccc
     2641 gatggcgacg tcgagggcga ccttctcgtt gggtgaccac tcggcataca catcggggta
     2701 gggggcgaac gcttcgagga tctccgtgct gggggtcccg gggtagcccg cgccgagcac
     2761 cgcgcctgcc tcccatgcgg ctcgcgcgat ggcttcgtta cccgacagga gacgcttcat
     2821 gggtgggtcc tcctcgaccg gcccgcggga tcgccggggc gctacttcgc acgatctaac
     2881 gcggtcgcgg cgtcgtccag tgccataggt ccctcgactc gggggttccc caaagtcgcg
     2941 ttctgacgtt gatcgaggtt aatgtcagac gtcacaaggg gtcaaaacgt tgatttgggt
     3001 ttcctattct taactaaggt gtcagtatgg aggtatgaga agcgctctcc ccgcactcag
     3061 gcaggcatcc aaaagaagcc tggccctggc catcaccttc ctcacgatgg gccttgtcgg
     3121 actggtcatc ccagcctcag cgtcggccat gccgtacgtc gactcgaccc cctccgcatc
     3181 cagtctcccc gccggtctcc aacccctgcc ggatgaggat ctgaacgcct cctccaagcg
     3241 cctgtcgctc gccgcgtgca acgcgctggt ccagaaaggc atcatctacc acccggccgg
     3301 gtggaagttc tcctgctcct ccagtcgggt cggcaagagc tacaacgcct ggaccttccg
     3361 gcgcgaaacg gtcgtcaagc caggtatgac actggccaag accaaggccg tccttgcgca
     3421 cgaagaagcc cacatctggt cgctgtcagt gctcacgcca gcgcagatga cgtggttcag
     3481 tcgacagttg ggcaagaggt ccttcttcga cggcacgacg aggacgatgc cggccgaggt
     3541 ctgggcagcg tcccaggctg cgtgtgctgg ctaccctcag ggcccgtaca agcgagtctc
     3601 gtgctcgctg cttcgagaga ccctgaggca ctgaggtcct ccgcttctct gtgtgggcgt
     3661 catctccgcc cacacagaga agcggaactc gtggggccga gtagatcgac gctgtcctat
     3721 cgcgccgatg atatgtggtc gacctttcgg aaggtcgtcg actattgagc ggaccagctg
     3781 ccaacgagcg atagcgacgc gcatcccttt ctgtcgagta gctaccccgg tctgtcatgc
     3841 ccgccatgtc gctcgctgac tactgaccct cagcgggcgg tgtggctctt gtgaacaaag
     3901 gcccgatgga taacgcctcg gcggatccgt tgggtcgtca attggggccg ccgcgtgctg
     3961 ggtgatccag cccctggagc agacctctcg tcacggccaa ccccccctcc tgggtcggga
     4021 cgagctgtct ccaggcgggc ccgacatcgt cgcatccgca gatgtgaccc cccttgcttt
     4081 acccgcgcac ttttgaacac ttttgagagg ttcgtcatga ggagcaccag tcctcgtgtt
     4141 gcagatcgcc acggcttggt cgcggcaaca ccgtatcggc ccgctcacat ggaagatgtg
     4201 aacgcttttc cgctccgcag gtcatccgtc ggcgtgtctc gccgcacgct tctcgtcggc
     4261 gtcgctgggg gcctccttgc cacagcgctt gacatcgacg ctgtcgccaa ccccctactc
     4321 gaccctacgg gaaccccgcc ggcgggcttc accttccacg gccgagcccc agatgcgctc
     4381 gcacacacgg cctacagcgg agggtgcata cacaccttga tcgggtacca gggtcgcatc
     4441 tactacggct acgggaacta cggtgcaaac tccgggtcgg cgtccggaat gggcactaac
     4501 gtttcctact tcggttcggc gggaggtttc ggttcatcat tcacctcgtt ccgaaccgag
     4561 gagattaaca cgtacaggcg gtttggtggt gcgctattcg ccccatgtat cgatccctcg
     4621 tctggagctc ccgcaggcaa tagctttgcc tcgaatctag acggcagatg ggccgccttt
     4681 gtgggtccgc cggccgagca cgtttttgac gtgtgcgact cccagaacgc gggggagtat
     4741 ctgcttgccg gctcgtccgg cggcttctcc gcagtggtgt ggcgcacgac cgattatggc
     4801 cggacgtggg ctgaactgtg gcgtgagccg gaggagtccg cgtccatgga cgggtacgag
     4861 cgtttctatt ggctgacgcg tattggtaac cgcatcttta tgcgcgctga tcccgggaca
     4921 aagggtacca agcccccgat gcgatactac gacacgagac gtcagcgcat gaacaaggta
     4981 ccaaacagtg ccgtgacggt cggtggatcg aaattctacc cggttatcct tggagaccgc
     5041 gtgcaaaggg cgaatcaggt ttgtggcgat ggacgaatcg catatttcat cactgatggc
     5101 aaagtatttg cctttgatgg cgctcaatcc agtcaggtat cttcagtgtg gggcgttctg
     5161 tcgcccggcg atgacggacg gatttacctc aaggacgcca ctggcgtcta cgtcttgacg
     5221 ggaaccacaa agaccacgat cttggctcag gccgaccttc ccggtgtgag catgacagtt
     5281 gcgaatggcc gtatctggct aggcggtagc caatccgaga tctggtctcg tccgctttga
     5341 attctcccca gcctcgcgcc caatccgctc agccccaggt ggccagccac acgagctcgc
     5401 tgggccgcca agcgcggacg gctgccaacg ctcaaccgaa gggacctgac cagccatgct
     5461 cacaccccag cccggcaccc cgtttccgcc cgcgcagcgg ccgaaggaat tcttccaaga
     5521 agggacccag cggactagcc gcgagtgcgg accgataaat gaggccaccc atcacctcat
     5581 caccgcgaga ctgcgcaccg aattctccgc cgctcgcata ggggttgctg ctctcggttg
     5641 agggcgccga tccgatggtg ccgtgcaggc gtcggttggt gaaccggtcg acccagccgg
     5701 cggcgcggtc atcttccgcc cgcacgggcg ggccctccgc ggtcagctga cgcctaccgt
     5761 cccccggtga cggtccaggc cgcgtcacgg accgcgtgga tcacaagcgc gtcactgacc
     5821 cgtgcggatg ttcgacggcg cgggggtcga gttccccccg aagaaggacg tcgcggtgac
     5881 gcacaaatct attggcgcaa gtggctctag gagggcccat gagaagggca cggattcttg
     5941 cggtggccgc agttgcagta gcggcagttg cgaccgcatc gctcggcatc atctacgcga
     6001 cgggagttgc tgcggttgcg actgcaacgc agcaggccca tgtcggaagc atcggttgga
     6061 tgaccccagt tcagccggga tcggtgctcg gccaacctgg ccagaaccgg gccatcgagg
     6121 ccatcaaact gactgaactg ccgacaggta gcaccatctc agcccatgtg tcgagtatcg
     6181 gctggatggc tccggtgggg ccaggaatgg ttgctggaac gactggtcgc ggcctacaaa
     6241 tggaggcggt gaaggtcacg gtccccagcg gctgcacggt cacgtaccag gcgtacgtcc
     6301 gtggtcaggg ctggtcggct tctgtgtcca acggagccat tgctggcacc accggcaaag
     6361 cgttggcctt ggacgggttg agggtcaacg ccgtcacatg cgccacgtcg accaccagca
     6421 ccacgacccc accggtcgcc ggaacgcagc atgcgttctt tggcggggct tgggcgccag
     6481 cggtacgcgt tggcgaggtc attggccaac ctggccaaaa ccgggccatc gaggccatca
     6541 aactgaccga actgccgaca ggtagcacca tctcagccca tgtgtcgagt atcggctgga
     6601 tggctccggt ggggccagga atggttgctg gaacgactgg tcgcggccta caaatggagg
     6661 cggtgaaggt cacggtcccc agcggctgca cggtcacgta ccaggcgtac gtccgtggtc
     6721 agggctggtc ggcttctgtg tccaacggag ccattgctgg caccaccggc aaagcgttgg
     6781 ccttggacgg gttgagggtc aacgccgtca catgcgcgac aacgccacct cctgcgagcg
     6841 tgaaattcgc cgctgtggga gatatcggga ccgactcgga gaatggttcc actgccgcga
     6901 gttccatgct caaaggacta ggagccaacg gctacaattt cgcactgaac ctgggggatc
     6961 tcgggtacaa caacaacccc ggggtcgagc agaactactg cacgttcctc aagaacaatg
     7021 ccggtagcag tcttcaatgg ctgatggtcc ccggcaacca cgagggaact gacccgacat
     7081 acggcggccc ggagggcgac tacaggaagt acgtcgccta ctgtgcagga tcaaccccgc
     7141 cagccggtgt tgtcggaaat tacccgtacg actactacgt agaccggggg aacgtcagaa
     7201 tcatcatgct ctccccaaac atcagcaatc ctgcagggaa gctgacgtac cgcaacggca
     7261 cggccgaaca ggccacgctg ggcacctgga ttcagggggc aaaatcggct ggcaagttcg
     7321 tggtcgttgg tgcccatgag ccgtacttga ccatcggcga acaccgactg accacagaca
     7381 atctggcaga ccctcagttc gcggcgttct tggtagcgca gcgggttgac ctgtcgctgt
     7441 ctgggcatga ccacaacatc agtcgtgcct atcagctcgg agggtccgtc acatcgcaga
     7501 cctctcccac agtcgtggac agggacggat cgttcgtct
//
LOCUS       DAOMZY010000156         7522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_9244_length_7522_cov_6.465113, whole genome shotgun sequence.
ACCESSION   DAOMZY010000156 DAOMZY010000000
VERSION     DAOMZY010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7522
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9244_length_7522_cov_6.465113"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..147
                     /locus_tag="PLK64_09745"
     tRNA            75..147
                     /locus_tag="PLK64_09745"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:108..110,aa:Thr,seq:ggt)
     gene            177..250
                     /locus_tag="PLK64_09750"
     tRNA            177..250
                     /locus_tag="PLK64_09750"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:211..213,aa:Met,seq:cat)
     gene            409..579
                     /gene="rpmG"
                     /locus_tag="PLK64_09755"
     CDS             409..579
                     /gene="rpmG"
                     /locus_tag="PLK64_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPV80073.1"
                     /translation="MASKTSDVRPKITMACTECKDRNYITKKNRRNDPDRLALAKFCP
                     KCGKHTEHRETR"
     gene            1091..1534
                     /locus_tag="PLK64_09760"
     CDS             1091..1534
                     /locus_tag="PLK64_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HPV80074.1"
                     /translation="MPVNPEFAGRVYPPSAPYGVSAAKIKEFAEAVGSTDPVHTDRAV
                     AQARGYRDVIAPPTFAVLIAQRAEMEFIFDPEAGVDFSRVVHGEETFVHHRPLTAGDE
                     IVGQLTVDSVKAAGGHTMIVTRAELSAGDEPVCTALSTIVVRGGE"
     gene            1534..1983
                     /locus_tag="PLK64_09765"
     CDS             1534..1983
                     /locus_tag="PLK64_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HPV80075.1"
                     /translation="MSAATMGAGSRREFSAVSVGEVLPAKTVHIDRARLVQYAGASLD
                     RNPIHWDERFAVKVGLPNVIAHGMFTMGAAVTVVTDWAGDAGAVVRYGTRFTKPVVVP
                     YETGADVVFSATVTALDEATRQATVEISALCGTDKVLGRATALVQLA"
     gene            1985..3058
                     /locus_tag="PLK64_09770"
     CDS             1985..3058
                     /locus_tag="PLK64_09770"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPV80076.1"
                     /translation="MQEAFAAPLAALTTMRVGGPADRLVTAESIDDIVDAVREVDDAD
                     EPLLLLSGGSNLVVADDGFRGTVLRIASRGVRVESLDRCSGATVRVAAGEPWDDLVVR
                     AVTEGWSGIEALSGIPGCVGSTPVQNVGAYGQEVADTIAQVRVWDRDREAVRTLTASE
                     CEFAYRDSVFKHRGARLVVLDVVFQLRLADLSAPVGYADLAAALEVAEGERVPLADAR
                     EAVLAQRRRRGMVLDAADHDTWSCGSFFTNPVLSESDYQTLVDRVADRLGPAGPAVPR
                     WSAPGGVKTSAAWLIERAGFGKGFGMPGPAALSTKHTLAVTNRGSATAAEIVALARTV
                     RDGVQDAFGVALVNEPVLVGLSL"
     gene            complement(3068..3823)
                     /locus_tag="PLK64_09775"
     CDS             complement(3068..3823)
                     /locus_tag="PLK64_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80077.1"
                     /translation="MRSMPRRRATTAAPVVVTVLTVGLLVGGCSTGSPTAAPTTATTA
                     NATATVTESATATSTDSSAATATATSTATDPAAPVPTARTATPPPAPTATAPPLTAAL
                     PTEFTPMGQVIKDDSLGHEITVLRMARGLPWPEGSSGQSAAFELLALELRLTPGTKYT
                     ATLRELDFALITGSQYPNRPDPLIDAQLALAGWALLPTEVPAGQNATGWVVFKVDPKA
                     ATSIRLDYTRPEISVAGSRTRFPKKVFSLQIVG"
     gene            complement(3869..4882)
                     /locus_tag="PLK64_09780"
     CDS             complement(3869..4882)
                     /locus_tag="PLK64_09780"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HPV80078.1"
                     /translation="MTTLPKAHLHLHFTGSMRIDTLLDLAVARGVRLPDALTDNWPPH
                     LHATDERGWFRFQRLYDLARSCVRGEEAMRRVVAEAAADDAAEGSRWLELQVEPSSYA
                     PHVGGLTPALEIVLDAAREASIDSGIGVGIVVAASRIRHPLDARTLARLAARYAGDGP
                     GTVVGFGLSNDERRGATADFASAFDIARRAGLALVPHGGELLGPTSVQETLGALRPDR
                     LGHGVRSVEDPRVLDEVAEAGVALEVCPGSNLAMGVYATPREVPLLELVGHGIPVALG
                     ADDPLLFGSRLVDQYTLARDALRCSDRQLADLARDSLRASRAPEQVRLTALRDVDAWL
                     ARG"
     gene            complement(4915..5448)
                     /locus_tag="PLK64_09785"
     CDS             complement(4915..5448)
                     /locus_tag="PLK64_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5709 domain-containing protein"
                     /protein_id="HPV80079.1"
                     /translation="MSTDEAAFDNDTIGDGLEVWSVDDEDQLQQGDTLIDRGVDDVLD
                     EGFSPPDRPRGSYAYGTTAAEQRGHETIDQRILQEEPDPYSAYGAPDDESGEQRGRKE
                     KIGGDDPDSIYADADYVGQAGRVRSGRIVASAEDGNTEFENDVFAGDVGIDGGGASAE
                     EAAMHIIDLDSDEDLED"
     gene            complement(5535..6983)
                     /locus_tag="PLK64_09790"
     CDS             complement(5535..6983)
                     /locus_tag="PLK64_09790"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80080.1"
                     /translation="MPSDPRGLRGDAPALDAWLAFQGGAPTPFTIPGHKQRLDLVGDV
                     VAGDVPLFAGLDTMSQAHGVLAAAEARAARLWGADVCRFSVGGSTHGNQALALAVGAP
                     GDRVIVSRTLHRSLLLGLVLAGLTPVWVRPDVDDLGMPRGIPPRALTDALALAPDAVA
                     VFIGDPSYVGTLTDIEGLAAVAHAHRGSRARGGADGIPLVVDAAWGAHFGFHPALPGH
                     ALARGADALVTSAHKTLPAWSQGALVLARTERIDPARLAAAVDATATTSPSGAILASI
                     DASRALVERDGPALLGDLLGQVADLRARLRAVPGLVVLDGPGVDPVKVCLVLSGTGAD
                     GTQIEADLIARRMPVELADRDTIVAMVTLADTPTTLRRLEDALVELVEVHRGTPRPTA
                     ASIAWSVAPQQVCPPRAAFFARQETVAAARAVGRTCGELVAPYPPGIPVLAPGELITE
                     ETMSALRAARAAGTRIAYAADPTLATVRVLAD"
     gene            complement(7025..>7522)
                     /locus_tag="PLK64_09795"
     CDS             complement(7025..>7522)
                     /locus_tag="PLK64_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPV80081.1"
                     /translation="NGVAKTYAMTGWRVGWMIGPKDVIAAATNLQSHLTSNVNNVAQR
                     AAITALSGDLAAVDAMKVAFDRRRRTMVAMLNEIDGITCPTPSGAFYVYPSVKGVLGQ
                     TIRGARPLTSAELASLILDEVEVAVVPGEAFGPSGYLRLSYALGDADLVEGVTRIQSL
                     LAEAH"
BASE COUNT         1111 a         2578 c         2709 g         1124 t
ORIGIN      
        1 ccgatttcta ctgtggccac gcgagcgtgt actctctgga ccgcactcct ggggcccctt
       61 gggcggccag gggcgcccca atagctcagt cggcagagcg tttccatggt aaggaaaagg
      121 tctagggttc gattccctat tggggctcgg gatcaggcgt cgcgccggat cgccacggcg
      181 ggatagctca gttggtcaga gcgcacgact cataatcgtg aggtcgcggg atcgagcccc
      241 gctcccgcta ctgcttccga tcggggccgc ccagcgtccg gtcggaccgc ggatttcccg
      301 gttcgacgcc cgatcacgta cccttgagcg ttgcgcgcgc gcagccgagt gtttgcgccc
      361 gcgtccgaca gaccagccca tcgacagacc agcagcgagg tagccaccgt ggccagcaag
      421 acgagcgatg tccggccgaa gatcaccatg gcgtgcaccg agtgcaagga ccgcaactac
      481 atcaccaaga agaaccgccg caacgacccc gaccggttgg cgctggccaa gttctgcccc
      541 aagtgcggca agcacacgga gcaccgcgag acccgctaag gctcgcagcc ggcatacgtc
      601 aaggccgcgt ctcccctggg gagccgcggc ctttgtcgtg cccgggagcg gggggaccgg
      661 cgcggccgac cgggcgacgc cccgacccgg cggcgcgggt gccccagatc acgggacgtc
      721 ggcccttccg tcggaggcga cacgctgcta atgtcttcgg tttgcttcac ccaagatgaa
      781 ctgctaacct tacgaaggtg aacaacgggt gaacggccgg tgaggtctaa accccagcgc
      841 accacgagcc gccaccacga gggtgacgac gtgacattcc caagggggga ttgacatgcg
      901 caaactcttt tctctgtacc gcagcgacta caagtcgacg ccgtgcatcg cgagcctggc
      961 cgtgcttccg ctgaagcccg aggcctgagc cgactaatgg tcgaagcccc cgtcgcctgg
     1021 cgacgggggc ttcgccgttg tcaggcttgc gacggcgccg tcgcgtcgag ggtggcctag
     1081 gcttccccgt atgccggtca atcccgagtt cgcgggacgg gtctacccgc ccagcgcccc
     1141 ctacggcgtc agcgccgcca agatcaagga gttcgcggag gcggtgggct cgaccgatcc
     1201 ggtgcacacg gatcgggctg tcgcccaggc ccgcggctac cgcgacgtca tcgccccgcc
     1261 gacgtttgcg gtgctcatcg cgcagcgcgc cgagatggag ttcatcttcg atcccgaggc
     1321 tggtgtcgac ttcagccggg tcgtccacgg cgaggagacc ttcgttcatc accgccccct
     1381 gaccgccggt gacgagatcg tcgggcaact caccgtcgac tcggtcaagg cggccggtgg
     1441 acacacgatg atcgtcaccc gcgccgagtt gtcggccgga gacgagcccg tgtgcacggc
     1501 cctgtccacg atcgttgtca ggggaggcga gtgatgtccg ctgccaccat gggggccggt
     1561 tcgcgccggg agttctccgc cgtctcggtc ggcgaggtcc tgcccgccaa gaccgtgcac
     1621 atcgaccggg cccggctagt gcagtatgcc ggcgcctccc tcgaccgtaa cccgatccac
     1681 tgggacgagc ggttcgccgt caaggtcggt ctgccgaacg tcatcgccca cggaatgttc
     1741 accatgggcg cagcggtcac ggtcgttacc gactgggccg gagatgctgg agcggtcgtg
     1801 cgttacggca cccggttcac caagcccgtc gtcgtgccct acgagaccgg tgcagacgtc
     1861 gtgttctcgg cgacggtcac cgcgctggac gaagcgaccc gccaagcgac cgtcgagatc
     1921 tccgcgctgt gcgggaccga caaggtgctc ggtcgggcca cggcgctcgt ccaactcgcc
     1981 tgacgtgcag gaggctttcg cagcgccgct cgcggcgttg accaccatgc gggtgggcgg
     2041 gcctgctgat cgcctcgtca cggccgagtc gatcgacgac atcgtcgatg ccgtccgcga
     2101 ggtcgacgac gccgacgagc cgctgctgct gttgtccggc ggctcgaacc tcgtcgtggc
     2161 cgacgacggc ttccgcggca cggtgctgcg gatcgccagt cggggcgtgc gggtcgagtc
     2221 gctcgaccgg tgcagcggcg cgacggtgcg ggtcgcggcg ggcgagccgt gggacgacct
     2281 ggtcgtccgg gccgtcaccg agggctggtc ggggatcgag gcgctgtccg ggatcccggg
     2341 gtgcgtcggg tcgacgccgg tacagaacgt cggggcctac ggccaggagg ttgccgacac
     2401 catcgcccag gtgcgcgtct gggaccggga ccgtgaagcg gtgcggaccc tgaccgcctc
     2461 cgagtgcgag ttcgcctatc gcgactcggt cttcaaacac cgaggcgccc ggttagtcgt
     2521 cctcgatgtg gtcttccaac tgcgcctggc cgacctgtcg gcgccggtgg ggtatgccga
     2581 tctcgcggcg gcgttggagg ttgccgaggg cgagcgggtg ccgttggccg atgcacgtga
     2641 ggcggttctg gcccagcggc ggcgccgtgg catggtcctc gacgccgctg accacgacac
     2701 ctggagttgt gggtccttct tcaccaatcc ggtgctctcc gagtccgact accagaccct
     2761 ggtcgatcgg gtcgcggacc ggctgggccc ggcggggccg gcggtgccgc ggtggagcgc
     2821 ccccggaggc gtcaagacca gtgcggcctg gctgattgag cgggccggct tcgggaaggg
     2881 cttcggtatg ccgggcccgg ctgccctgtc gaccaagcac accctcgcgg tgaccaaccg
     2941 cggcagcgcg accgccgcgg agatcgtcgc cttggcgcgc accgtccgcg atggggtgca
     3001 ggacgcgttc ggggtggcgc tggtcaacga gcccgtgctt gtcgggctca gcctctgact
     3061 gcgccggtca cccgacgatc tgcaggctga agaccttctt agggaatctc gtcctgctcc
     3121 cggcgaccga gatctccggg cgggtgtagt ccaaccggat cgacgtcgca gccttggggt
     3181 cgaccttgaa gacgacccac ccggtggcgt tctggccagc cgggacctca gtcggcagca
     3241 gggcccaccc ggcgagcgcc agttgtgcgt cgatgagagg gtcgggccga ttcgggtact
     3301 gcgatccggt gatgagggcg aagtccagtt cgcgcagcgt cgcggtgtac ttcgtgccgg
     3361 gtgtcaatcg cagctccaac gccaacaact cgaacgccgc agactgcccc gaggacccct
     3421 ccggccaggg caggccgcga gccatccgca gcacggtgat ctcgtggccg agcgagtcgt
     3481 ctttgatcac ttgacccatc ggcgtgaact cggtcggcag ggcggccgtg aggggaggcg
     3541 cggtcgcggt cggtgcgggt gggggagtgg ctgttcgtgc tgtcgggact ggggccgccg
     3601 ggtcggtcgc cgtgctcgtc gcggtcgccg tcgcagcgct cgaatcggtc gaggtggctg
     3661 tcgcgctctc ggtgacggtc gccgttgcgt tagcggtggt ggcggtggtt ggcgccgctg
     3721 tcggtgaccc ggtcgaacaa ccgccgacga gcaggccgac ggtcaggacg gtcaccacga
     3781 cgggggcggc ggtggtggct cggcggcgcg gcatactcct cacctgcacc agcctaaacg
     3841 gctccgggag tgcgccccga cgggtcggtc acccgcgagc cagccacgca tcgacgtcgc
     3901 gcaacgccgt gagtcgcacc tgctcaggcg cgcggctggc gcgcagcgag tcgcgggcga
     3961 ggtcggccag ttgccggtcc gagcaccgca gggcgtcccg ggcgagggtg tattgatcga
     4021 ccaggcgcga cccgaagagc aagggatcgt cggcccccag agccacgggt atgccgtgtc
     4081 ccaccaactc gagcaggggc acctcacggg gcgtcgcgta gacccccatc gcgaggttgc
     4141 tccctgggca gacctccagt gcgactccgg cctcggcgac ctcgtccagc acccgagggt
     4201 cctcgaccga tcgcaccccg tgaccgagtc gatccggtcg gagtgcaccg agggtctcct
     4261 gaacggacgt cggacccagc agctctcctc cgtgcggcac cagggccagc cccgctcgcc
     4321 gcgcgatgtc gaaggccgat gcgaaatccg ctgtcgcgcc acgccgttcg tcgttggaca
     4381 gcccgaaccc gacgaccgtc ccggggccgt cgccggcata ccgggcggcg agccgggcga
     4441 gggtgcgggc gtcgagcgga tggcggatcc gcgaggccgc gacgacgatc ccgacgccga
     4501 tgccggagtc gatcgacgcc tcgcgggccg cgtcaaggac gatctccaag gcgggcgtga
     4561 ggcccccgac atgcggcgca taggacgacg gctcgacctg cagttccagc cagcgggagc
     4621 cctcggcggc gtcgtccgcg gcggcttcgg ctaccacccg acgcatcgcc tcctcgcccc
     4681 gcacgcacga gcgggccagg tcatagaggc gttggaagcg aaaccacccg cgctcgtcgg
     4741 tggcgtgcag atgcgggggc cagttgtcgg tcagcgcatc gggcagccgc accccgcggg
     4801 cgaccgcgag gtccagaagt gtgtcgatcc gcatcgatcc ggtgaaatgc aggtgcagat
     4861 gagccttggg gagggtcgtc agggaccggg gcccggaggc tgggtcgatg gtgctcagtc
     4921 ctcgaggtcc tcgtcgctgt ccaggtcgat gatgtgcatc gccgcttcct cggccgacgc
     4981 gccgccgccg tcgatcccca cgtctccggc gaacacgtcg ttctcgaact ccgtgttgcc
     5041 gtcttcggcg ctcgcaacga tgcgaccgct gcggacccgc ccggcctgtc cgacatagtc
     5101 cgcgtcggcg tagatcgagt cggggtcgtc gccgccgatc ttctccttgc ggccccgctg
     5161 ctcgcccgac tcgtcatcgg gcgcgccgta ggcggaatac gggtcgggct cctcttgaag
     5221 gatgcgctgg tcgatcgttt cgtggccacg ctgctcggcc gccgtcgtgc cgtaggcata
     5281 cgacccgcgg gggcgatcgg gaggggagaa accctcgtcg agaacgtcgt cgacgccacg
     5341 gtcgatgagg gtgtcacctt gctgcagctg gtcctcgtcg tcgaccgacc acacctccag
     5401 cccatcgccg atggtgtcgt tgtcgaaggc agcctcgtcg gtgctcatac acaccactct
     5461 gccacttccc tgcgcatcaa gcgacgtggt gaaggcctca agatccccac ggagatcctc
     5521 cccgcgtcaa cgcctcaatc ggcgaggacg cgcaccgtcg cgagggtcgg gtcagcggca
     5581 tacgcgatgc gcgttccggc ggcacgtgcg gcccgcagcg cggacatcgt ctcctcggtg
     5641 atcagctcgc ccggagccag caccggtatg ccgggtggat agggcgccac caactcacca
     5701 cacgtccgcc cgacggcgcg agcggccgcc accgtctcct ggcgggcgaa gaacgccgcg
     5761 cgtggcgggc agacctgttg cggggcaacg ctccatgcga tcgaggcggc cgtgggccgg
     5821 ggcgtgccgc ggtggacctc gacgagctca acgagggcgt cctcgagccg acgcaacgtt
     5881 gtgggggtgt cggcgagggt caccatggca acgatggtgt cgcggtcggc caactcgacc
     5941 ggcatccgcc gcgcgatgag gtccgcctcg atctgcgtgc cgtcggcgcc ggttccggac
     6001 aggacgaggc acactttgac tgggtcgacg cctggcccgt cgagcaccac caaccccggc
     6061 accgcgcgca gccgagcccg gaggtccgcg acctgcccga ggaggtcgcc gagcaaggcc
     6121 ggcccgtcgc gctccaccag ggcgcgactg gcgtcgatgc tggcgaggat cgccccggag
     6181 gggctcgtcg tggcagtggc gtcgacggcg gcagcgaggc gggccgggtc gatccgctcg
     6241 gtccgggcca ggacgagcgc gccctgcgac cacgcaggca gcgtcttgtg tgcgctggtc
     6301 accaatgcgt cggcgccgcg agccagcgcg tggccgggca gggccgggtg gaatccgaag
     6361 tgcgcccccc aggcggcatc gacgacgagg ggtatgccgt ccgccccgcc ccgcgcccgg
     6421 ctgccgcggt gggcgtgagc cacggcggcc aggccctcga tgtcggtgag ggtcccgacg
     6481 tacgacggat ccccgatgaa gacggcgacg gcgtccggtg ccagggccaa cgcgtcggtg
     6541 agggcccgcg gaggtatgcc gcgcggcata cccaggtcgt ccacgtcggg gcgcacccag
     6601 acgggggtga ggccggcgag gaccagcccg agcagcagcg agcggtgcaa ggtgcggctg
     6661 acgatgaccc ggtcacctgg tgcgccgacc gccagggcga gggcctggtt gccgtgagtg
     6721 gacccgccga cggagaaccg acagacgtcc gcgccccaga gcctggccgc ccgcgcctct
     6781 gcagcggcca gcacgccgtg ggcctgagac atcgtgtcca gacccgcaaa gagcgggaca
     6841 tcgccggcga cgacatcgcc gacgaggtcc aggcgctgct tgtgcccagg gatggtgaag
     6901 ggggtcggcg cgcccccctg gaatgccagc caggcgtcca gggcgggcgc atcaccccgc
     6961 agcccgcgag gatcgctcgg catcgcgccg cgctcgcgtc gtcaggccgc cggccgagcg
     7021 gaggtcagtg cgcctcggcg agcaacgact ggatccgggt cactccctcg accaggtcgg
     7081 cgtcgcccag ggcataggac agccggaggt agccgctggg gccgaacgcc tcacccggga
     7141 cgaccgcgac ctcgacctcg tcgaggatca gggaggcgag ctcggcggag gtcagcgggc
     7201 gagcgccacg gatcgtctga ccgaggacac ccttgaccga cgggtagacg tagaacgctc
     7261 ccgagggggt ggggcaggtg attccgtcga tctcgttgag catggcgacc atggtccggc
     7321 ggcgacggtc gaaggcgacc ttcatcgcgt cgaccgccgc gaggtcaccg ctgagggcgg
     7381 tgatggcggc ccgctgggcg acgttgttga cgttgctcgt caggtggctc tgcaggttgg
     7441 tggctgcagc gatgacgtcc ttgggaccga tcatccagcc gacccgccac ccggtcatcg
     7501 cgtaggtctt ggcgaccccg tt
//
LOCUS       DAOMZY010000157         7483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_9293_length_7483_cov_5.845584, whole genome shotgun sequence.
ACCESSION   DAOMZY010000157 DAOMZY010000000
VERSION     DAOMZY010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9293_length_7483_cov_5.845584"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..1399
                     /locus_tag="PLK64_09800"
     CDS             212..1399
                     /locus_tag="PLK64_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV80082.1"
                     /translation="MIRFEGVTKRYAGAPGHAPSVAVDDLTLEAPTGQITVLVGPSGC
                     GKTTTLRMVNRMITPTSGSIWLDGRDTAGLDEAELRRGIGYVIQHAGLFPHRTVLDNV
                     ATVPRLLGWDKAKARARAAEMLDLVGLDAAYARRYPSQLSGGQQQRVGVARALAADPP
                     VMLMDEPFSAVDPVVREQLQDEFLRLQRELGKTILFVTHDIDEAIKLGDQVAVLRVGG
                     RLAQLDTPSRLLADPVDDFVADFVGRDRGYRALGFREAPPLALTDEPTVPLGASVGAA
                     RAATTNRWLLVVDEGGAPVGWVEPERLVAPIGREHLHRGGTVARADGSYRAVLDAALS
                     APSGRGVLVDGAGVLVGTVTANRVVAAMSRPAAAAVGEAASGGGAGGIPETARGDHAG
                     SVT"
     gene            1396..2124
                     /locus_tag="PLK64_09805"
     CDS             1396..2124
                     /locus_tag="PLK64_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPV80083.1"
                     /translation="MTWVWEHLGDIGGYAVSHALLAGIPLLVGLALALPLGWLARRFP
                     RVAPVIVGGSGLLYTIPSLALFILMPLLLGSRILDSINVYVAMTIYTVALLVRSVVDA
                     LGSVPEATALAATSMGYRRVGRALAVELPLAVPVLASGLRVAAVSNVSIVSVASLIGY
                     PQLGYYLTDGYQRSFPLEIGVGIVGCVLLALVFDLAIRGAEWALTPWARAAASSSAAG
                     SRYSAIAKRWRNTSTRQSEGVGTR"
     gene            2121..2906
                     /locus_tag="PLK64_09810"
     CDS             2121..2906
                     /locus_tag="PLK64_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPV80084.1"
                     /translation="MIAQIWQWLIAPEQWSGSRGIPTRLLEHLGFSALSLLLAAAIAI
                     PVGLWVGHTGRGRSVVTIANAARAVPSLGLLFAVAMFVGPRITSNLAFVIPSVVVLVL
                     LAIPPLLSGADSGVRSVDPAVRDAAYGMGMTGREVLWRVELPCALPLLLSGVRAATLQ
                     VIATATIAAAVSLGGLGRYLIDGLAVSDYAQMAGGAILVAALALAIDGLLALAQRLLV
                     SPGLATRPSQRRHGKFPRARGVAGGRGRVLAPAALTTHGRDLT"
     gene            2903..3835
                     /locus_tag="PLK64_09815"
     CDS             2903..3835
                     /locus_tag="PLK64_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPV80085.1"
                     /translation="MRHLRARLALTTAAVVAAGLALSACGASSNPTVAASTPAGSASG
                     ATTSVVIGSADFSESQILAEVYAAALNAKGIKATTRPNIGSREVYLKAIQDGSVDIVP
                     EYTGSLLNYLDKTATAKAPDEVYAALGKAVPTGLVALDKSAAEDKNSIVVTKDTAAKW
                     SLTQIGDLVAHQAEIVVAAPPEFQTRVQGLPGLKDVYSFVPKDFRPLKGQAVVDALVN
                     GQTQAANVFSTDPSILANGFVTLADPKALFGSDNVVPLVRKDKNSAALAAALNAVSAK
                     LDTATLTKLVSQVDVDKKDAVAVAKAWVASVGLG"
     gene            complement(4114..6414)
                     /locus_tag="PLK64_09820"
     CDS             complement(4114..6414)
                     /locus_tag="PLK64_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019174014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPV80086.1"
                     /translation="MSALQITAIVLCFTIPLLGWALLFRQSYRFYRLFQTGAPDPTRN
                     DHPATRVWTVLREFLGHTRLARLPIVAIAHWFTALGFFLLFATLVNAFFQLIWADFRL
                     PIVGGFVPFEWLIELFAGIGFIGICVLIWIRQRNHPRSAEGAAGRRSRFFGSVWWQAY
                     YVEFTIFAVMLCIMVLRTLEARLVQIDHPTESLVLHFPLSGWGAQWLWQGADKGTVEQ
                     LIYLVAMLKILVSFAWMITISLTPTMGVAWHRFLAFVNIYFKRHADGRTSLGAVQPMM
                     VAGKPFDMEAMEDLDEDTTLGVGKIEEFTWKGLLDFTTCTECGRCQSQCPAWNTDKPL
                     SPKLLVMTLRDHAHAKAPWLMATEEKRKDLPAHTQKLADAPLVGSLGYDVEHPLTAYD
                     PLGLEGVIDEDVLWSCTTCGACVEQCPVDIEHVDAIIDMRRYQNLIASAFPSEFGTLY
                     KNLEKNSNPWGLAPRMRMDWAKGLPFDVPVVGSDVEDCSSVDYLFWVGCAGAFEDRAK
                     RTSRAVAELLHTAGVTYAVLGDGEACTGDSARRSGNEMLFQMLAMQNVEMLNEVGATK
                     IVVTCAHCYNALKNEYPQVGGNYEVVHHTQLLNKLVREKRLVPVSRPGESAGSKDAST
                     AGSVTYHDPCYLGRHNGVYTPPRELLGALPGVTMTEMPRHGEKSFCCGAGGARMWVEE
                     KLGTRINLNRTSEAVATGAARIAVGCPFCKVMLSDGVNKFVETGELTEGAVEVVDVAQ
                     MLLAAVRRGEEPEAAAASAPEPVPSA"
     gene            complement(6485..6991)
                     /locus_tag="PLK64_09825"
     CDS             complement(6485..6991)
                     /locus_tag="PLK64_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPV80087.1"
                     /translation="MSATYPVKTCLWFDTEAEAAARLYTSLVPGSEILTISHYGSENQ
                     YGETGSVLEVDFRLGTQQFVALNGGPHFTHTPAASIQVFCPDQPELDRIWDGLLADGG
                     EPSQCGWLTDRFGLSWQIIPTRLGELAGDPDPGRSSRTIAAMLKMSKLDIAALEAAAD
                     ADPDTDDA"
     gene            7108..>7483
                     /locus_tag="PLK64_09830"
     CDS             7108..>7483
                     /locus_tag="PLK64_09830"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPV80088.1"
                     /translation="MSTTSVRSDAFEASAAAKIAETHPDADLLALRASFNLVRLSTLH
                     MHRLESEVHRDAGWSMAGFRVMFCVWVAHDLEPREIARFSGLSRAAVSSVLNTLERDG
                     LVERHRESSDRRLVTVRLTAEGE"
BASE COUNT         1103 a         2554 c         2618 g         1208 t
ORIGIN      
        1 cactcgctcc gagctcttcg aggtcctcga cctggctcgc gccgggctta tccacgtcga
       61 gacggagacg ttcgcgctcg atgacggccc cacggcatac gagcgactgc acgcggggac
      121 gttgcgcggc cgcgccgtca tcgtcccctg atctaccggg tctcagcgcc ttgacggagc
      181 ccctgtcaga gggctgggtt agcgtgtcgg aatgattcgc ttcgagggcg tgaccaagag
      241 gtatgccggt gcgcccggtc acgcccccag cgttgccgtc gatgacctca cgctcgaggc
      301 gccgaccggg cagatcaccg tgctcgtcgg gccgtcgggg tgtggcaaga cgacgaccct
      361 gcggatggtc aaccggatga tcaccccgac atccgggtcg atctggctcg acgggcgtga
      421 cacggctggg ctcgacgagg ccgagttgcg gcgggggatc ggctacgtca tccagcatgc
      481 cgggctcttc ccgcaccgca ccgtcctgga caacgtggcc accgtgcccc ggctgctcgg
      541 ctgggacaag gccaaggctc gcgcgcgggc ggccgagatg ctcgacctcg tggggctcga
      601 cgcggcatac gcgaggcgct atcccagcca gctctccggg gggcagcagc agcgggtggg
      661 ggtcgcgcgc gcgctggccg ccgacccgcc ggtcatgctc atggacgagc cgttctcggc
      721 ggtcgacccg gtggtccgcg agcagttgca ggacgaattc ctgcggttgc agcgcgaatt
      781 gggcaagacg atcctcttcg tcacccacga catcgacgag gcgatcaagc tcggcgacca
      841 ggtggcggtg ctgcgggtgg gtggccggct ggcccaactc gacacgccgt cgaggctgct
      901 cgccgacccg gtggacgact tcgtcgccga cttcgtgggg cgcgatcggg gctatcgggc
      961 gctgggcttc cgggaggccc cgccgctggc gttgaccgac gagccgaccg tgccgctggg
     1021 ggcgagcgtc ggggcggccc gagcggcgac cactaatcgg tggttgctag tcgtggacga
     1081 gggtggcgcg ccggtcggct gggtcgagcc tgaacggttg gtcgccccga tcggccgcga
     1141 gcacctgcat cgcggtggca cggtggcccg cgccgacggc tcctatcggg cggtgctcga
     1201 cgcggcgttg tcggcgccct cagggcgtgg cgtgctcgtc gacggcgctg gcgtgctcgt
     1261 cggcacggtc accgccaacc gggtcgtcgc ggcgatgagt cgcccggcgg ctgccgcggt
     1321 tggtgaggca gcgagcggcg ggggcgcggg cggcataccg gagactgcgc gcggtgacca
     1381 cgcggggtcc gtgacgtgac ctgggtctgg gagcacctcg gcgacatcgg tgggtatgcc
     1441 gtgtcccacg ccctgctcgc cggcatcccc ctgctcgtcg ggctggcgct cgcgctgccg
     1501 ctcggatggc tggcgcggcg gttcccgcgg gtggcgccgg tgatcgtcgg ggggagcggg
     1561 ctgctgtata cgatcccgag cctggcgctg ttcatcctca tgccgctgct gctgggcagc
     1621 cggatcctcg actcgatcaa cgtctatgtg gcgatgacga tctataccgt tgcgctgctg
     1681 gtgcgttcgg tcgtcgatgc cctcgggtcg gtgcctgagg cgacggcact ggcggcgaca
     1741 tcgatgggat atcggcgggt cgggcgggcg ctggcggtcg agttgccgct ggccgtgccg
     1801 gtgctggcgt cggggttgcg ggtcgcggcg gtgtcgaacg tcagcatcgt gtcggtggcc
     1861 tcactcatcg gctatccgca gctcggctat tacctcaccg acggctacca gcgctcgttc
     1921 ccgctcgaga tcggggtggg gatcgtcggg tgcgtgctgc tggcgctggt cttcgacctg
     1981 gcgatccgcg gcgccgaatg ggcgctgact ccgtgggccc gcgccgccgc ctcctcgtcc
     2041 gctgctgggt cgaggtattc cgccattgcc aaacgatggc ggaatacctc gacccggcag
     2101 tcggagggcg tgggcacgcg atgattgccc agatctggca gtggctcatc gcgcccgagc
     2161 agtggtcggg gtcgcgcggc ataccgacgc ggctcctgga acacctcggg ttctcggcgc
     2221 tgtccctgct cctcgcggcg gccatcgcca tccccgtcgg cttgtgggtc gggcacaccg
     2281 gccgaggccg atcggtcgtg accatcgcca acgcggcgcg cgcggtgccc agcctcggtc
     2341 tgctcttcgc cgtcgcgatg ttcgtcggcc cgcggatcac ctccaacctc gccttcgtca
     2401 tccccagcgt cgtcgtcctc gtcctgctcg ccatcccacc tttgctctct ggcgccgact
     2461 ccggggtgcg gtcggtcgac ccggcggtgc gcgacgcggc atacggaatg gggatgactg
     2521 gccgcgaggt gctgtggcgg gtcgagctgc cgtgtgcgct gcccctgctg ctctccgggg
     2581 tgcgcgcggc gacgctgcag gtgatcgcca cggcgaccat cgcggcggcg gtgagcctcg
     2641 gcgggctcgg ccgctacctc atcgatgggc tggccgtcag tgactatgcc cagatggcgg
     2701 gtggggcgat cctcgtcgcc gcgctggccc tcgctatcga cgggctgctc gccctcgccc
     2761 aacgactgct cgtgtcgccg gggctggcga cgcgtccgtc ccaacgccgc cacgggaagt
     2821 tccctcgggc ccgtggtgtt gcaggcggca ggggtcgcgt cctggcgccc gctgctctga
     2881 ccactcacgg aagagacctg acatgagaca cctacgtgcc cgcctggccc tcaccaccgc
     2941 agcggttgtt gccgccggac tcgccctgtc ggcgtgcggc gccagctcca accccaccgt
     3001 cgccgcctcg acgcccgcgg gctcggcgtc cggcgccacc acctcggtcg tcatcgggtc
     3061 ggccgacttc tccgagtcgc agatcctcgc cgaggtgtat gccgcggcgc tcaacgccaa
     3121 gggcatcaag gccacgaccc gccccaacat cggcagccgc gaggtctacc tcaaggccat
     3181 ccaggacggg tcggtcgaca tcgtccccga atacaccggg tcgctgctga actacctcga
     3241 caagaccgcc accgcgaagg cccccgacga ggtgtatgcc gcgctcggca aggccgtccc
     3301 taccggcctc gtcgccttgg acaagtccgc cgccgaggac aagaactcga tcgtcgtcac
     3361 caaggacacc gccgccaagt ggagcctcac ccagatcggt gacctcgtcg cccaccaggc
     3421 cgagatcgtc gtggccgcgc cgcccgagtt ccagacccgc gtccagggcc tgccgggcct
     3481 caaagatgtc tacagcttcg tgcccaagga cttccgcccg ctcaagggcc aggccgtcgt
     3541 cgacgccctg gtcaacggtc agacgcaggc ggcgaacgtc ttctccaccg acccgtcgat
     3601 cctcgccaac gggttcgtca cgctggccga cccgaaggcg cttttcggct ccgacaacgt
     3661 cgtgccgctg gtgcgcaagg acaagaactc ggctgcgttg gcggccgcgc tgaacgccgt
     3721 ctcggccaag ctcgacacgg ccaccctcac caagctggtg agccaggtcg acgtcgacaa
     3781 gaaggacgcg gtcgccgtgg ccaaggcctg ggtagccagc gtcggcctcg gctgaaccct
     3841 ggcggcgccg acgggccgat cgccggccca accgccgggc tcctgaactg atcgtgtggc
     3901 tgggcccccc gtgtccggcg agtggccgac ccccacaccc ccctttgaaa aaccgcgtac
     3961 ctcaggtacg caccccgtac ctgaggtacg gggaaacttc acccctcctg ccgcctccaa
     4021 ctcaggtggg accagccgac ccgcaaggcc aagagcgtgt gcagccacac gatgttccgg
     4081 ggagacaacc gccaggtatg ccgcccgctc aactcacgcg ctcggcaccg gttcgggggc
     4141 gctcgccgca gcggcctcgg gctcctcgcc gcggcgcacc gcagccagca gcatctgagc
     4201 gacgtcgaca acctcgaccg caccctcggt cagctcgccg gtctcgacga acttgttgac
     4261 gccgtccgag agcatgacct tgcagaacgg gcagccgacg gcgatccggg cagccccggt
     4321 ggcgacggcc tcggaggtgc ggttgaggtt gatccgggtg ccgagcttct cctcgaccca
     4381 catccgggca ccaccggcgc cgcagcagaa cgacttctcg ccatggcgcg gcatctcggt
     4441 catcgtcacg cccggcagcg caccgagcaa ttcgcgcggc ggggtgtaga cgccgttgtg
     4501 ccggccgagg tagcacgggt cgtgataggt gaccgagcca gcggtcgagg cgtccttgga
     4561 cccggccgac tcgccaggcc gcgacaccgg cacgaggcgc ttctcccgca ccaacttgtt
     4621 gagcaactgg gtgtggtgga cgacctcgta gttgccgccg acctgcgggt attcgttctt
     4681 cagcgcgttg tagcagtggg cgcaggtgac gacgatcttg gtcgcgccga cctcgttgag
     4741 catctcgacg ttctgcatcg cgagcatctg gaagagcatc tcgttgccgg agcggcgagc
     4801 ggagtcaccg gtgcaggcct cgccgtctcc gagcacggca tacgtgacgc cggcggtgtg
     4861 gagcaactcg gcgacggcgc gcgaggtgcg cttggcgcgg tcctcgaagg cacccgcgca
     4921 gccgacccag aacaggtagt cgaccgacga gcagtcctcg acatcggacc cgacgaccgg
     4981 gacgtcgaac ggcagcccct tggcccagtc catccgcatc cgcggggcca gcccccacgg
     5041 gttggagttc ttctcgaggt tcttgtagag cgtcccgaac tcgctcggga aggcgctcgc
     5101 gatgaggttc tggtagcggc gcatgtcgat gatcgcgtcg acgtgctcga tgtcgaccgg
     5161 gcactgctcg acgcaggcgc cacacgtggt gcacgaccac aggacgtcct cgtcgatgac
     5221 gccctccaga cccaacgggt cgtatgccgt caacgggtgc tccacgtcgt aacccagcga
     5281 tcccaccagc ggcgcgtcgg ccaacttttg ggtgtgggcg gggaggtcct tgcgcttctc
     5341 ctcggtcgcc atgagccacg gcgccttggc gtgcgcgtgg tcgcgcagcg tcatgacgag
     5401 caacttcggc gagagcggct tgtcggtgtt ccacgccggg cactggctct ggcaacggcc
     5461 acactcggtg cacgtcgtga agtcgagcag acccttccag gtgaactcct cgatcttgcc
     5521 gacgcccagc gtggtgtcct cgtcgaggtc ctccatcgcc tccatgtcga acggcttgcc
     5581 ggcgaccatc atcggctgga cggcaccgag cgaggtgcgc ccgtcggcgt gccgcttgaa
     5641 gtagatgttg acgaacgcca ggaagcggtg ccacgcgaca cccatcgtcg gggtgagcga
     5701 gatggtgatc atccacgcga agctcacgag gatcttgagc atcgcgacga ggtagatgag
     5761 ctgctcgacg gtgcccttgt cggcgccctg ccacagccat tgggcgcccc atccgctgag
     5821 cgggaagtgc aggacgagcg actcggtggg gtggtcgatc tgcacgagcc gcgcttcgag
     5881 ggtgcgcagc accatgatgc agagcatgac ggcgaagatc gtgaactcga cgtagtaggc
     5941 ctgccaccac accgacccga agaaccgcga gcggcgcccc gcggcgccct ccgcggaccg
     6001 ggggtggttg cgctgccgga tccagatgag cacgcagatc ccgatgaacc cgatccccgc
     6061 gaacagctcg atgagccact cgaacggcac gaagcccccg acgatcggca gccggaaatc
     6121 ggcccagatc aactggaaga acgcattgac gagggtcgcg aagagcagga agaaccccag
     6181 tgcggtgaac cagtgcgcga tggcgacgat cggcagccgc gcgagccggg tgtgcccgag
     6241 gaactcgcgc agcaccgtcc acacccgcgt ggccgggtgg tcgttgcgcg tcgggtccgg
     6301 cgctccggtc tggaacagcc gatagaagcg gtaggactgc cggaacagca gtgcccaccc
     6361 caacaacgga atcgtgaagc acagcacgat cgcggtgatc tgcagggcgc tcatggttgt
     6421 gaagtctagt gccggctaag cgcttgctca ccggctcggc gtgtgacgca cctcgcgcag
     6481 tgacctaggc gtcgtcggtg tcggggtcgg cgtcggcggc ggcctcgagg gcggcgatgt
     6541 cgagcttgct catcttgagc atggcggcga tcgtgcgtga actgcggccg gggtcggggt
     6601 cgccggccaa ctcgccgagc cgcgtcggga tgatctgcca ggacaggccg aagcggtcgg
     6661 tgagccagcc gcattgcgac ggctctccgc cgtcggcgag gaggccgtcc caaatccggt
     6721 ccagctcggg ctggtcgggg cagaagacct ggatcgacgc agccggggtg tgggtgaagt
     6781 gcgggccacc gttgagggca acgaattgct gtgtgccgag ccggaagtcg acctcgagca
     6841 ccgacccggt ctcgccgtat tggttctcgg agccgtagtg gctgatggtg aggatctcgc
     6901 tgccgggcac gagcgaggtg tagaggcgcg cagccgcctc agcctcggtg tcgaaccaca
     6961 ggcaggtctt gacgggatag gtcgcggaca cgtgcggctc cttcgactgg gcggatgtgc
     7021 cttcggggtg tgagggtatc ggggggagtt cggtgtggcg accaaggtca tggcttgatt
     7081 gacagtcaaa actgacatca tctaggcgtg agcacaacga gcgttcggtc cgatgccttc
     7141 gaggcatccg ccgccgcgaa gatcgccgag acccatcccg acgccgacct gctcgcgctg
     7201 cgggcctcgt tcaacctggt gcggctgtcg accctacaca tgcaccggct tgagtcggag
     7261 gtgcatcgcg acgccgggtg gagcatggcc ggcttccggg tgatgttctg cgtctgggtc
     7321 gcgcacgacc tcgagccgcg cgagatcgcc cgtttctccg ggctgtctcg ggccgccgtc
     7381 tccagcgtgc tcaacaccct cgaacgcgac ggcctggtcg aacggcaccg ggagtcctcc
     7441 gatcgccgtc tggtgacggt gcgactcacc gccgagggcg aaa
//
LOCUS       DAOMZY010000158         7437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_9354_length_7437_cov_5.785424, whole genome shotgun sequence.
ACCESSION   DAOMZY010000158 DAOMZY010000000
VERSION     DAOMZY010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7437
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9354_length_7437_cov_5.785424"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PLK64_09835"
     CDS             <1..443
                     /locus_tag="PLK64_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HPV80089.1"
                     /translation="PVQVSASVTGGDGTGGTGWLGGLGVLGWLGVAVWIVGFAFEAVG
                     DAQLRRFKADPANRGRVMDQGLWAWTRHPNYFGDAMVSWGLWLVAAGHWPGVLTVLSP
                     VLMTVLLVRVSGAKLLEKAMGARPGYADYISRTSSFLPRPPRRR"
     gene            complement(780..1472)
                     /locus_tag="PLK64_09840"
     CDS             complement(780..1472)
                     /locus_tag="PLK64_09840"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPV80090.1"
                     /translation="MTVPLGWEPIRGVIFDYHSTLAHGGDPATWLGSAWAATDRPGTP
                     EVAFGQTGYAALLDFLDHIWEHAGRVDPDNGRDRDPVRHREVFDQVVALRPEVGADLA
                     DALYASMLDQWQAYPDAAPTLAQLRGRGIRTAILSNMGVDLHPVLARQGLAPLLDAVV
                     LSWECGAVKPEAEAFHAALRALGLAPAQVLMVGDNSLDDAGAAAIGIRTLLLPRTMGA
                     GARGLGIVTRLV"
     gene            complement(1483..1623)
                     /locus_tag="PLK64_09845"
     CDS             complement(1483..1623)
                     /locus_tag="PLK64_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80091.1"
                     /translation="MDATLWDDRYAASDLVWSAEPNPTVATLPADLPPGRALDVAARR
                     SA"
     gene            1776..2987
                     /locus_tag="PLK64_09850"
     CDS             1776..2987
                     /locus_tag="PLK64_09850"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPV80092.1"
                     /translation="MAQLTSRLRGRKALTGIVVLAVLLAAGATWWVRSRSTASAASAV
                     PTSAVAASVQTLSQSVSATGTINPKVQSNLRFGSSGTVAAVDVKVGDAVTAGQQVAAI
                     TTTDLQTALDLAEANVSSAQANLTTVKASSATTTSQLNAAKSQLAAAQAKLTTAQTNL
                     TNARLTSPVAGTVATVNIAVGDQVSAGGTSSGGTGSSGSGSSGAASAAIVVITTDAWI
                     VSTSVTSADLPLLKQGMQAQITPSGSRTLVFGTVSTIGVIATSTSGVATFPVTIAVTG
                     SPKGLYAGSSASVAIIVKEVADAITVPTAAIRTEGGKTVVSKLVDGAAVTTPVQVGLV
                     QGSFTQVTSGIAEGDQVVVTGFSGPGSGSGGQSTSTRTRGSGGTGGLGGVGFPPGGQG
                     DLPAPPPGAVP"
     gene            3062..3742
                     /locus_tag="PLK64_09855"
     CDS             3062..3742
                     /locus_tag="PLK64_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV80093.1"
                     /translation="MLELRGVSKTYTSGSLDVAAVRNVDLVVNHGDYVAIMGPSGSGK
                     STLMHIIGCLDVPTSGSYWLAGVDVAGMGESDLAVVRNQRIGFVFQQFNLLPTMSAWR
                     NVELPLIYAGVSRAERRRRAIDALTRVGLADRVDHRPGELSGGQQQRVSVARALVTEP
                     DLILADEPTGNLDSTSTAEMLELFAELNQHGRTIVLITHEHDVAEAADRMVVMRDGSL
                     DEPTGARR"
     gene            3739..4968
                     /locus_tag="PLK64_09860"
     CDS             3739..4968
                     /locus_tag="PLK64_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV80094.1"
                     /translation="MTWLETLRTAYDSLRTRRLRSALTMLGILIGIAAVMLTVGLGQG
                     AQKQVTDQINKLGSNLLIVTPGSTTSSSGVRGGRGSATTLTIDDAAVLADPSVAPDIA
                     RVAPTSGGSQSLVAGTTTWTSNVVATNAEWLGVRARSVAQGRFFTAAEVTSESSVAVL
                     GSTTAAQLLGSTNAVGQTITVGGQNVTVIGVLNTEGSTTGGDQDDLVVMPLTTYASRL
                     STSANPRSVSTIYIEAKDATSLSAAYQETTNALLSAHGVTLATQDFSVSSQQAIVETA
                     TSTSRILTVLLGSIAAISLLVGGIGVMNIMLVSVTERIREIGLRKALGATPRIILRQF
                     LVEASLLGLAGGLAGLALGMLGAAILPRLVNFPVAISPVAAAAGLGVSLVIGITAGVY
                     PASRAARLAPIEALRSE"
     gene            5168..5974
                     /locus_tag="PLK64_09865"
     CDS             5168..5974
                     /locus_tag="PLK64_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80095.1"
                     /translation="MSGLIAAVDGTTMQVQSSTKQTAVGWTATTSVARTAAGTLADIV
                     AGSCVVVRSASSDGSAPDAGGGVTATSVQVSAAVNGACADVFGGGIRGGGGIPSGMPT
                     GQPGVDQLPPGATVGAPGGPGGRVPGGFGVVGLVTQVGSGTFTVASLTRVGDGGPGTA
                     SAAPAQTTPVTVTTSASTTVTTTKVAAAGDIAVGLCATAIGPTDDIGKVTATSIVLRD
                     AVNGACTSTGGFDGPGAPGAGFPGGGGPGGGGPGGGGPGGAAPTSAATNG"
     gene            5967..>7437
                     /locus_tag="PLK64_09870"
     CDS             5967..>7437
                     /locus_tag="PLK64_09870"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPV80096.1"
                     /translation="MAERVLRIKRRRWLPMVGVGGAILLVAVGGTYVANGVSAGSETA
                     ALRTGQVTTGSIEQTLTLTGSVTRVSQMTAHFPVAGTVTGVSVAVGDIVTAGQALATL
                     DTGPLNQAVLDAKSTLDQAKATLANDTTTASSSSSSSSASSSGTSSSSPTSSSSRTSA
                     SSSPSTSAGGGGGGGGQNQTTSALAAVAAAQALAKTACAPVLPVATATPTSVPTSTAT
                     STSVPTSTATSTATSTQTATSTVTSTTTTSPPTAQEIADCLGALSAVTAAQQAAGVAL
                     TSYAGSLSAAAAQAAAQAAAQAAAQAAAQAQAADQAKAAAAAAQQAAAAVRSSSGTVS
                     EAKLITDRANVTNAEAALVKAQANLAAATLTAPMAGRVGSIAWAVGDTATAANGIQIV
                     GSGAAKVTIQVPAANLAAIAVGQAARVTVTGIPLMDGTVSQVALLPTSTTGSSASTYA
                     VDVLVPNAPDAVGTGGKASVAVVTTKVDGVLTVPASAVTG"
BASE COUNT         1091 a         2688 c         2575 g         1083 t
ORIGIN      
        1 tgcccgtcca ggtgagcgcg agcgtcaccg gcggcgacgg caccggcggg accgggtggc
       61 tcggcgggct gggggtgctc ggctggctgg gggtggctgt gtggatcgtc gggttcgcgt
      121 tcgaggcggt cggggatgcg cagttgcgca ggttcaaggc cgatccggcc aatcgcggcc
      181 gagtcatgga ccaggggctg tgggcgtgga cccgtcaccc caactacttc ggcgacgcca
      241 tggtcagctg gggcctgtgg ctcgtggccg ccgggcactg gccgggcgtg ctcaccgtgc
      301 tctcacccgt gctcatgacc gtgttgctcg tgcgggtgtc tggagcgaag ctgctcgaga
      361 aggcgatggg tgcccgaccg ggctacgccg actacatctc gcggaccagt agcttcctgc
      421 cgcggccgcc ccggcgccgc tgatcgcgtg ctgccagaaa tgcacagcgg gtgggacggc
      481 aatcccccgc cgccccaccc gccatgcatt gactagccct ccagatgtgg gctgcatccg
      541 gtgcgctggt catggtggat gggccagctc tcccttctgg cccacccacc acgtgtgtgg
      601 tactgcggtt cgcccttccg gctggtcccc tccgaaccct ccggaagggc gaaccgctga
      661 cccgctcccg cctctgagat ccccccgggc ctcagttctt cgagtcggat gtaggacggt
      721 gaccccccat ggtcatgaca gacgtttcga gagattttca aaaacttttt ggagggctct
      781 cagaccaggc gggtgacgat gcccagaccc cgtgcgccag cgcccatggt gcgcggcagg
      841 agcagggtgc ggatcccaat ggcggccgcc ccggcgtcgt cgagcgagtt gtccccgacc
      901 atgaggacct gggccggcgc cagccccagc gcacgcagcg ccgcgtggaa cgcctccgcc
      961 tccggcttga ccgcgccgca ctcccacgag aggacgaccg cgtcgagcag gggggccaga
     1021 ccctggcggg ccagcaccgg gtgcaggtcc actcccatgt tggacaggat cgccgtgcgt
     1081 atgccgcgtc cgcgcaactg cgccagcgtc ggcgccgcgt ccggataggc ctgccattgg
     1141 tcgagcatcg aggcatagag ggcatcggcc aggtcggcgc ccacctctgg gcgcagggcc
     1201 acgacctggt cgaagacctc ccggtggcgg acggggtcgc gatctcggcc gttgtcgggg
     1261 tcgacccggc ccgcgtgctc ccagatgtgg tcgaggaagt ccaggagggc ggcatacccg
     1321 gtctgcccga aggcgacctc gggcgttccg ggccggtcgg tggccgccca tgcgctcccc
     1381 agccaggtgg ccgggtcacc gccgtgcgcg agggtgctgt ggtagtcgaa gatcaccccg
     1441 cggatcggtt cccaaccgag ggggacggtc acggccgaca ccttaggccg accggcgggc
     1501 ggcgacgtcc aaggcgcgac cgggagggag gtcggcgggc agggtggcga ccgtggggtt
     1561 gggttccgcc gaccacacga ggtcggaagc ggcataccgg tcgtcccaca gcgtcgcgtc
     1621 catatcggcg attctcgcgc acagccacgc ggtgtgaaac gcacaggtga cacacaggtt
     1681 gtgctcggtt gggtccaagg ttcctgtggt ggattgacgt ggagttgcga accacgccag
     1741 tagcatgctg gccgtagcgc aaccgggaag gtgccatggc ccagctcacg tccaggttgc
     1801 gaggccgcaa ggccctgacc ggcatcgttg tcctcgcagt tctcctggcc gccggcgcga
     1861 cctggtgggt gcgttcgcgc tcgaccgcgt ccgccgcctc agccgtgccg acctcggcgg
     1921 tggcagcatc ggtgcagacc ctcagccagt cggtgagcgc gaccgggacg atcaacccca
     1981 aggtccagtc caacctgcgg ttcggctcgt ccggcacggt ggcggcggtc gacgtcaagg
     2041 tcggcgatgc ggtgaccgcg ggccagcagg tcgccgcgat caccaccacc gatctgcaga
     2101 ctgccctcga cctggccgag gccaacgtct cctcggcgca ggccaacctc acgacggtca
     2161 aggccagcag cgccacgacg accagccagc tcaacgccgc gaagtcccag ctcgccgccg
     2221 cccaagcgaa attgaccacc gcccagacca acctcaccaa cgccaggctc accagcccgg
     2281 tcgcgggcac ggtcgctacc gtcaacatcg ccgtcggcga ccaggtgagt gccggcggca
     2341 cgtcctcagg cggcaccggc tcgtccggct ccgggtcatc gggcgccgcc agcgcggcga
     2401 tcgtcgtcat caccaccgac gcctggatcg tgtcgacctc ggtgacctcc gccgacctgc
     2461 cgctgctcaa gcagggaatg caggcccaga tcacgccgtc cggctcgcgg acgctcgtct
     2521 tcggcacggt ctcgacgatc ggggtcatcg cgacctccac cagcggggtc gcgaccttcc
     2581 cggtcaccat cgcggtcacc ggctctccca agggcttgta tgccgggtcg tcggcgagcg
     2641 tggccatcat cgtcaaggag gtcgcggacg cgatcaccgt gccgacggcg gccatccgca
     2701 ccgagggcgg caagactgtc gtcagcaagc tcgtcgacgg ggccgccgtg accacgcccg
     2761 tgcaagtggg cctggttcag ggcagcttca cccaggtgac cagtgggatc gccgagggcg
     2821 accaggtcgt cgtcaccggc ttctcggggc cggggtccgg ctcgggcggg cagagcacca
     2881 gcacccgcac tcgcggatcg ggtgggaccg gaggacttgg tggcgtcggc ttccctcccg
     2941 gtgggcaggg cgatctgccc gcaccgccgc ccggggcagt gccgtgaccg ccgagactcc
     3001 gtgggcgacc gatctcgccg gagacgccgc cgccgttgag cgaccgttga ccgacggcgc
     3061 cctgttggag ctgcgcggcg tcagcaagac ctacacctcg ggttccctgg acgtggccgc
     3121 tgtccggaac gtcgacctcg tcgtgaacca cggcgactac gtcgcgatca tgggcccctc
     3181 cgggtccggc aagtcgaccc tcatgcacat cattgggtgc ctcgacgtgc cgacgtccgg
     3241 aagttactgg ctggccgggg tcgacgtcgc cgggatgggc gagagcgacc tcgccgtcgt
     3301 gcgcaaccag cggatcggct tcgtcttcca gcagttcaac ctgctcccga cgatgtcggc
     3361 ctggcgcaac gtcgagctgc ccctcatcta tgccggggtg tcgcgtgccg agcggcgccg
     3421 gcgggccatc gatgcgttga cccgggtcgg cctggccgac cgggtcgacc accggccggg
     3481 tgaactctcc ggcgggcagc agcagcgggt gtcggtggct cgcgccctgg tgaccgagcc
     3541 tgacttgatc ctcgccgacg agcccaccgg caacctcgac tccacgtcca ccgccgagat
     3601 gctcgaactt ttcgccgagc tcaaccagca cggtcgcacg atcgtcctca tcacccacga
     3661 acacgatgtc gccgaggccg ccgatcggat ggtcgtcatg cgcgacggca gcctggatga
     3721 gccgacagga gcacgtcggt gacctggctg gaaacgctgc gcacggcata cgactccctg
     3781 cggacccgcc gcctgcgatc ggcgctgacg atgttgggca tcctcatcgg gatcgccgcg
     3841 gtcatgctca ccgtggggtt gggccagggc gcccagaagc aagtgaccga ccagatcaac
     3901 aagcttggct ccaacctgct catcgtcacg ccgggcagca cgacgagctc ctcgggcgtg
     3961 cgcggtggac ggggaagcgc gaccaccctg accatcgatg acgcggccgt gcttgccgac
     4021 ccgagcgtgg cgccggacat cgcccgggtc gcgccgacca gcggcggctc gcagagcctg
     4081 gtggccggca ccacgacgtg gacgagcaat gtcgttgcga caaacgccga gtggctcggc
     4141 gtccgggccc gctcggtggc acaggggagg ttcttcaccg ccgccgaggt cacctcggag
     4201 agttcggtcg ccgtgctcgg ctcgaccacc gccgcccaac tgctcggctc gaccaacgcc
     4261 gtcggccaga ccatcacggt gggcgggcag aacgtcacgg tcatcggcgt gctgaacacc
     4321 gagggctcaa ccaccggtgg tgaccaggac gacctggtcg tcatgccgtt gacgacctat
     4381 gcctcacgcc tgtccacgag cgccaacccc cgcagcgtgt cgacgatcta catcgaggcg
     4441 aaggacgcga cgagtctctc cgcggcatac caggagacga ccaacgcgct gctatcggcg
     4501 cacggcgtca cgcttgccac ccaggacttc agcgtgagca gccagcaggc gatcgtcgaa
     4561 acggcgacct cgacctcccg gatcctcacc gtcctcttgg ggagcatcgc agcgatctca
     4621 ttgctcgtcg gaggcatcgg cgtcatgaac atcatgctgg tttcggtgac cgagcggatc
     4681 cgcgagatcg gtctgcgcaa ggctctcggg gcgaccccgc ggatcatctt gcgtcagttc
     4741 ctcgtcgagg ccagcttgct tgggctcgcc ggtggtctgg ccggcctcgc gctgggcatg
     4801 ctcggcgccg cgatcctgcc ccgattggtc aacttcccgg ttgccatctc gccggtcgcc
     4861 gccgccgctg ggctcggcgt ctcgctcgtc atcggcatca ccgccggcgt ctatcccgcc
     4921 agccgcgccg cgcgcctggc ccccatcgaa gcccttcgca gtgaatagga accccatgtc
     4981 ccgtatgccg cgcacccccc ttctccgact cgcacgaggc gccgctgtgc tcgcgtccgt
     5041 cgctgtggcg ggtttcgctt tgacggcctg tggcgctggg gcgtcaacgc ccaccacgac
     5101 cccggccgtg gcgaccgcga cccgcacggc agggcccggc cccggcgggg gtcaacggat
     5161 ccccggggtg tccggactca tcgctgccgt cgatgggacg accatgcagg tccagtcgag
     5221 caccaagcaa accgccgtcg gttggacggc aacgacgagc gtcgcccgaa ctgccgcggg
     5281 caccctcgcg gacatcgtcg ctggttcgtg cgtcgtcgtt cgcagcgcct caagcgacgg
     5341 cagcgcgccc gacgcgggcg gcggcgtcac cgccacttct gtgcaggtgt ccgcggccgt
     5401 gaacggcgcc tgcgccgacg tgtttggcgg cggcatccga ggtggcgggg ggataccctc
     5461 cggtatgccg accggccagc ccggcgtcga ccagctgccg cccggagcga ccgtcggggc
     5521 accggggggc cccggtggcc gagttccggg tgggttcggg gtcgttggcc tggtcaccca
     5581 ggtcggctcg gggaccttca ccgtggcgag cctgactcgc gtcggtgacg gcggcccggg
     5641 gacggcgtcg gcggcgccgg cccagaccac gcccgtcacg gtgacaacgt ccgcatcgac
     5701 gacggtgacg accacgaagg tcgccgcagc cggcgatatc gcggtggggc tgtgcgctac
     5761 cgcgatcgga cccaccgacg acatcggcaa ggtgacggcc acctcgatcg tcctgcggga
     5821 tgccgtcaac ggcgcgtgca ctagcaccgg tgggttcgac gggccgggtg cccccggcgc
     5881 ggggttcccg ggcggcggcg gccccggtgg cggcggtccc ggtggcggcg gtcccggtgg
     5941 cgcggccccg acctcggcgg ccaccaatgg ctgaacgggt ccttcggatc aagcggagac
     6001 gctggctgcc gatggtcggt gtcggtgggg cgatcttgct cgtggccgtc ggcggcacct
     6061 atgtcgccaa cggcgtcagc gcggggtcgg agacggccgc gttgcgcacc ggccaggtca
     6121 ccactggatc gatcgagcag actctgaccc tcaccggatc ggtgacccgg gtcagccaga
     6181 tgacggcgca tttcccggtc gctggcaccg tgacgggcgt gtcggtcgcc gtcggtgaca
     6241 tcgtcacggc cggccaggcg ctcgcgacgc tggacacggg cccgctcaac caggccgtcc
     6301 tcgatgccaa gtccaccctt gaccaggcca aagccacgct ggccaacgac accacgacgg
     6361 cgtcgtcgag ctcgtcgagc tcgtccgcct cgtcgtccgg cacgagctcc agcagcccga
     6421 cgtcaagcag cagccgcaca agcgcctcct cgagcccgtc gaccagcgca ggcggcggtg
     6481 gcggtggcgg tggccagaac cagacgacca gcgcgctggc cgccgtggcc gctgcccagg
     6541 cgttggccaa gaccgcctgc gccccggtgt tgcccgtcgc gacggccact ccgacgagcg
     6601 tgccgacgag cacggccacc tcgacgagcg tgccgacgag caccgcgacc tcgaccgcca
     6661 cgtccacgca gacggcgacc tcgaccgtca cctcgaccac gacgacctcg cccccgacgg
     6721 cgcaggagat tgctgactgc ctgggtgcgc tcagcgcggt gaccgcggct cagcaggcgg
     6781 ccggcgtcgc actcacctcg tatgccggtt ctctcagtgc cgccgcggcc caagccgccg
     6841 cccaagcggc tgcgcaagcc gccgcccagg ctgcggccca ggcccaggcg gccgaccagg
     6901 ccaaggcggc tgccgctgcg gcccaacagg ccgccgccgc ggttcgtagc tcaagcggga
     6961 cagtgtccga ggccaagctc atcaccgacc gagccaacgt caccaacgcc gaggcagccc
     7021 tggtgaaagc ccaggccaac ctcgcggccg ccacactcac ggccccgatg gccgggcggg
     7081 tgggttcgat tgcatgggcc gtgggcgaca ccgcgacggc cgcgaacggc atacagatcg
     7141 tcggatccgg tgccgcgaag gtgaccatcc aggtgcctgc cgccaacctc gcggcgatcg
     7201 ccgtcgggca ggccgcgcgc gtcacggtca ccggcattcc gctgatggac ggcaccgtct
     7261 cgcaggtcgc gttgctgccc accagcacga cgggctcgtc agcctcgacg tatgccgttg
     7321 acgtccttgt ccccaacgcc cccgatgcgg tcggcacggg cggcaaggca tccgtcgccg
     7381 tcgtcacgac caaagtggac ggcgtcctca ccgtgcccgc gtcggcagtc acggggg
//
LOCUS       DAOMZY010000159         7376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_9469_length_7376_cov_5.781314, whole genome shotgun sequence.
ACCESSION   DAOMZY010000159 DAOMZY010000000
VERSION     DAOMZY010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7376
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9469_length_7376_cov_5.781314"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="PLK64_09875"
     CDS             complement(<1..790)
                     /locus_tag="PLK64_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPV80097.1"
                     /translation="MDADLVVVGAGIFGLTVAQQCAQRFGVRVVVIDRRPHIGGNAYS
                     EEEPQTGIEVHRYGAHLFHTSNERVWEYVNQFTTFTNYVHRVYTNHRGEVYPMPINLG
                     TINQFFRSAYGPEQARALIAEQAAELGNREPANFVDKGISLIGRPLYEAFIAHYTAKQ
                     WQTDPELLGADIISRLPVRFTYDNRYFSDKYEGLPTDGYTAWLERVVDDPKIDVRLST
                     DFFDESQPVHQRAVVGNVPVVYTGPIDRYFGQSEGSLGWRTLDFE"
     gene            1198..2127
                     /locus_tag="PLK64_09880"
     CDS             1198..2127
                     /locus_tag="PLK64_09880"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HPV80098.1"
                     /translation="MVTAAYLVVVPLRAWNAVERVDNSPASSPAAGAGRNFLLVGSDS
                     REGLSAAEQAELSTGPDEGGKRTDSIMIVHVSDRGNPPVIVSIPRDSYVPIPGRGNSN
                     KINAAFAFGGARLLTETVQQATGLHIDGYLEIGFGGFARVVDSLGGVDICVARDMKDE
                     LAGIDLKAGCQVLDGKNALGYVRSRHSDPRGDLGRAERQRQFLGAVMKKAATPATVLN
                     PSRYLAFADAASAGLIVGSDTSLGDAVAIMQALRAVGNGEGLSLQVPIETAALQTKNA
                     GVAVKWNTTQAKALFTALRNDESLTAPPPGTAP"
     gene            complement(2158..4002)
                     /locus_tag="PLK64_09885"
     CDS             complement(2158..4002)
                     /locus_tag="PLK64_09885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPV80099.1"
                     /translation="MASDDPAASPGIGGVVAVVVTWNRRELLTRCLDAVLGQTHRPSR
                     VVIVDNASDDGTAALLADLYAALPGVQIVRTIENIGGAGGFALGLEFALGGAGDTAWL
                     LDDDTVPTAGALQALVGARTAYRTLTGTTPVLVASRAVWTDGRDHPMNTPRPKPGASA
                     DEAAAAAAVGCIPIRTASFVSILVDLDRTREVGLPEAGFFLWNDDFEFTARLVRGHRA
                     VYCPGSTVVHETKKFGGADADPGPRFHLEVRNKVWTFGRSRGLSAPEKVLYGGATARR
                     WARTIMRSTDRSTLIRGLGQGLRAGLSGGPRPTSVVLTEALGPDPAVGSLQPRPPQPR
                     REDGFAVLMSVYAGDRADFFEAAFRSVTADQTVPPDQVVLVIDGPLPQALNSRIEEVV
                     AATQVPVTLVRLPVNGGLGPALRAGLAHSEHELVARMDADDVSLPTRFEVQVPMVRGG
                     LDLVGSALLEFEDDPQVISRRRAVHTDPAQIQTGARFRQTLNHPSVVFRASAVEAAGG
                     YQDLPSLEDYLLFARMIHAGARVGNVAEPLVLYRVGAGSYARRGGLRLLRSELALQRE
                     FRRTGFTTRLQMMRNLVIRGGYRVAPEGLRKAGYGTFFARRDRGSGRG"
     gene            complement(4088..5257)
                     /locus_tag="PLK64_09890"
     CDS             complement(4088..5257)
                     /locus_tag="PLK64_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPV80100.1"
                     /translation="MWRVNPDFDAFQLSDSHRQLRAAVRELAEAQIEPYAADVDENAR
                     YPQEAHDALVASDFFAPHVPEEYGGAGADALAVCIIIEEISRVCASSSLIPAVNKLGS
                     MPLILAASEDLKHRYLTPMAEGKSGFCYGLSEREAGSDTSGMTCTATEQPDGSWILNG
                     HKAWITNAGVNDFYTVLAVTDPDGRRGANISAFVVEKSDPGFTFSEKERKLGIKGSPT
                     RELWFDNCTIPGDRMIGSRGEGLKIALRTLDHTRITIGAQAVGIAQGALDKAVTYVKE
                     RQQFGKNIAEFQGVQFMLADMAMELEAARQLVYVAAAKSERGDRDLSFFGAAAKCFAS
                     DVAMKVTVDAVQLMGGMGYTRDFPVERMMRDAKITQIYEGTNQIQRIVMARSVLA"
     gene            5392..5673
                     /locus_tag="PLK64_09895"
     CDS             5392..5673
                     /locus_tag="PLK64_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HPV80101.1"
                     /translation="MRAIWNGTVLAESDDTVVVEGNHYFPTAALRHDLVRPSDTHTVC
                     PWKGTASYFSVEVDGQVNEDAVWYYPDPKPKAAMVADRVAFWKGVRVSD"
     gene            complement(5696..6586)
                     /locus_tag="PLK64_09900"
     CDS             complement(5696..6586)
                     /locus_tag="PLK64_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HPV80102.1"
                     /translation="MSWTVDEIPDQSGRVVLITGANSGIGLESASVLADRGAHVILGC
                     RDPHKGAAAQRQIAGSTEVLTIDLSSLASVSEAAEALGGRQIDVLVNNAGIMAVPQEA
                     SVDGHELQFATNVLGPFALTSLLLDQITDRVVWVSSMMHRAGKLDLADPSFRHHRYNA
                     WRAYSASKLADLMLSYELQRRLTLAGSTVRSYAAHPGYAQTGLQRHTAVLSWPMVHQL
                     ESLLKVAQSADQGALPILYAATAPDLPPGSYVGPAGFGEMSGPPRIVGSSAASHDRDA
                     QRTLWSLCEGLTGTSPGLPG"
     gene            complement(6591..>7376)
                     /locus_tag="PLK64_09905"
     CDS             complement(6591..>7376)
                     /locus_tag="PLK64_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HPV80103.1"
                     /translation="GQMCTCMAYRNPAYLAKVAATVDIISGGRVEMGIGAGWYEHEWR
                     AYGYGFPEARDRLGALRDGVEIFRQMWTTGSATYAGTYYTVDGAICAPRPLQGTAAPG
                     SPANGIPMWVAGGGEKVTLKIAAKYADYTNFFGDLEEFTRKNEILKAHCAALGRDEAE
                     IVRSINVNVFIGETEVELNDRLEWYADHRIRVCGLPEAAVRREIDGLHGSVGCGTPEQ
                     VVERLAALRRAGLGYTIAYFADAAYDTTSIDLFERAVIPELKG"
BASE COUNT         1286 a         2450 c         2451 g         1189 t
ORIGIN      
        1 gctcaaaatc gagggtccgc caacccaagg acccctcaga ttgcccgaag taacgatcaa
       61 tcggccccgt atagaccacc gggacgttcc ccacaacagc ccgctgatgg acgggttgac
      121 tctcgtcgaa gaaatccgtc gacaagcgga cgtcgatctt cggatcgtcg accacccgct
      181 caagccatgc ggtgtaccca tccgtgggca agccctcgta tttgtcgctg aagtagcgat
      241 tgtcataggt gaagcgcacg ggaagccggc tgataatgtc ggcaccgagc agctcgggat
      301 cggtctgcca ctgtttcgca gtatagtgcg caatgaaggc ttcgtacagg ggccgaccga
      361 tgagagaaat gcccttatcg acaaaattgg caggctccct gttgccaagt tcggctgcct
      421 gctcggcaat cagagctctc gcttgctccg gcccgtaagc cgaccggaag aattggttaa
      481 tcgttccgag attaatcggc atcgggtaga cctcgccgcg atggttggta tacacccgat
      541 gtacataatt cgtaaatgtc gtgaactgat taacgtattc ccagacgcgc tcgttggagg
      601 tgtggaagag gtgcgcgccg tagcggtgta cttcaatgcc agtttgtggc tcctcctcgc
      661 tgtacgcatt tcctccgatg tgcggtcggc ggtcaatgac gacgacgcgt acaccaaacc
      721 gttgggcgca ttgttgggcg acagtcagcc caaagatgcc tgcaccaaca acgactagat
      781 ccgcgtccac cgggccatcc tcctgagtgc agtccatcgg gcctcccggc atcctacggt
      841 cttagccggg gccaacctgc caccgctctg gggtgacgac gtgcgagact ggcacccacg
      901 ctcaccacga aagggacgtc cgccgtgtcc gcttctgacc cgcacgaagg acgcgccgtt
      961 cccataccac agtcgatagg ccctcaagcc aacagaactg aggaccaggg ctacatcgtc
     1021 gacacccgcc gtaaggtcac gttctcccgg gaacctcgcc atcaaccccc acccgtcgcg
     1081 gcaccgccgt cggcaagaag acctgggcgg gtgagccagc cgcaaccccg aaacagtcga
     1141 ccaggtcgag cacggatcta cgtacgccgc ttcgtcgctg ccgtcgcact agtgggcatg
     1201 gtgactgcgg cttacctcgt ggtcgtcccg cttagggcgt ggaacgctgt agaacgtgtc
     1261 gacaacagcc ctgcaagcag tcctgcggca ggcgccggga ggaacttcct gctggtcggg
     1321 tcggacagtc gggagggtct ttccgcggcc gaacaagcgg agctctctac tggaccggac
     1381 gagggtggca aacggaccga ctccatcatg atcgtccacg tgtcagatcg cggaaaccct
     1441 ccagtcattg tgtcgatccc ccgcgatagt tacgtcccaa tccccggaag gggaaacagc
     1501 aacaaaatca acgcagcgtt tgctttcggc ggagctcggt tgctcaccga aacggtccaa
     1561 caggccacgg gtctgcacat tgacggctac cttgagatcg gtttcggagg gttcgctcgg
     1621 gtcgtcgaca gccttggcgg agtcgatata tgcgtggcgc gtgatatgaa agacgaattg
     1681 gccgggatcg acctcaaggc cggatgtcag gtcctggacg gcaagaacgc cctcggttac
     1741 gtgcgatcgc gtcacagcga cccccggggc gatctgggcc gagccgaacg tcagcgccag
     1801 ttcctcgggg cggtgatgaa gaaggccgcc acccccgcca ccgtcctcaa tcccagccgc
     1861 tacctggcct ttgccgacgc cgcgagtgcc gggctgatcg tgggttccga cacctcgctc
     1921 ggcgacgcgg ttgccatcat gcaggcgctg cgggcggtgg gaaacggtga aggcctgtcc
     1981 ttgcaggttc cgatcgagac agcagcgctc cagacgaaga acgcgggggt ggctgtcaag
     2041 tggaacacca cccaggccaa ggccctcttc accgcgctac gcaacgacga gagcctgact
     2101 gcgccgcccc ctggcactgc tccctgaccc cgtgcgccag cgcccgcgag acgcccctca
     2161 gccccgtcct gaaccgcggt cccgacgagc gaagaaggtg ccgtagcccg ccttgcgcag
     2221 cccttcggga gcaacccgat agccgcccct aatgaccagg ttgcgcatca tttggagccg
     2281 cgtcgtgaaa cccgtgcgcc ggaactcccg ttgcagagca agttcggacc gcaacaaccg
     2341 cagcccaccg cggcgggcgt aggaccccgc tccgacgcga tacagcacga ggggttcggc
     2401 gacattgccg acccgcgccc cggcatggat catccgggcg aacagcaggt agtcctcaag
     2461 ggagggaaga tcctggtagc cgcccgcagc ctcgacggca ctggcccgga agacgaccga
     2521 cggatggttg agggtctgcc ggaaccgagc cccggtctgg atctgggccg gatcggtgtg
     2581 gaccgcacgg cgtcggctga tgacctgtgg gtcgtcctcg aactcgagta gggccgaccc
     2641 cacgaggtcc aggcccccac gcaccatcgg cacttggacc tcgaatctgg tggggagcga
     2701 cacgtcgtcg gcatccatgc gggcgaccaa ctcgtgctcg gagtgggcca gcccggctcg
     2761 cagcgccggg ccgaggcccc cgttgaccgg taggcgaacc aacgtaaccg gcacctgcgt
     2821 ggccgcgacg acctcctcga ttcgggaatt caatgcttgg ggcaacggtc cgtcgatgac
     2881 gaggacgacc tggtccgggg ggacagtctg atccgccgtg acggatcgga aggccgcctc
     2941 gaagaaatcc gcccggtcgc cggcgtagac gctcatgagg acagcgaagc cgtcctcgcg
     3001 tcggggctgt ggcggccgcg gctgcaacga gccgaccgcc ggatcgggtc ccagtgcctc
     3061 ggtgagcaca acgctggtgg gtcggggccc accgctcaga ccggcgcgca ggccttgacc
     3121 caggccccgg atcagcgtgg accggtcggt ggacctcatg atggtccgag cccaacggcg
     3181 cgcagtggca ccgccgtaga ggaccttctc cggagcgctg agcccacgac tgcgcccgaa
     3241 cgtccagact ttgttacgca cttcgaggtg gaagcgcggg ccggggtcgg cgtcggcgcc
     3301 gccgaacttc ttcgtctcgt ggacgacggt cgacccaggg cagtagacgg cccggtgccc
     3361 acggacgagc ctggcggtga actcgaagtc gtcattccac agaaagaatc ccgcttccgg
     3421 caagcccacc tcacgagtgc ggtccaggtc gacgaggatg gagacgaacg acgccgtccg
     3481 aatcgggata catcccaccg cggcggcggc cgctgcctcg tcggcggacg ctccgggttt
     3541 gggccgcggg gtgttcatcg gatggtcacg gccatcggtc cacactgcgc gactggccac
     3601 caggaccggg gtggttccgg tgagcgtccg gtatgccgtc cgcgcaccca ccagggcctg
     3661 cagggctccc gccgtgggga ccgtgtcatc gtcgagcaac caggcagtgt caccggcgcc
     3721 gcccaaggcg aactcgagcc cgagggcaaa accgcccgca ccgccgatgt tctcgatggt
     3781 ccggacgatc tggacgcccg gcagggcggc atacagatca gcgagcagcg cagcggtgcc
     3841 gtcgtcagac gcgttgtcga cgatcacgac ccgcgacggt cgatgggtct gtcccagcac
     3901 cgcatctagg cagcgggtca gcagttcccg ccggttccac gtgacgacaa cggcgacgac
     3961 cccgccgatg cctggggatg cggcggggtc gtctgaagcc atacgcgaca tcctagggag
     4021 ccgagaactg atgcccggac cacgcccggc agacccggat ggccccgcct atcggtcaac
     4081 cggatcgtca ggccaagacg ctgcgcgcca tgacgatgcg ctggatctgg ttggttcctt
     4141 cgtagatctg ggtgatcttg gcgtcgcgca tcatccgctc gacggggaag tcccgggtgt
     4201 agcccatgcc gcccatcaac tggacggcgt cgacggtcac cttcatggcc acatcgctgg
     4261 cgaagcactt ggccgcggca ccgaagaagg acaggtcgcg gtcaccgcgc tcggacttgg
     4321 ccgctgcgac atagaccaac tgccgcgccg cctcaagctc catcgccatg tcggcgagca
     4381 tgaactgcac accctggaac tcggcgatgt tcttgccgaa ctgctgacgt tccttgacgt
     4441 aggtgaccgc cttgtccagc gcgccctggg cgatgccgac ggcctgggca ccgatggtga
     4501 tgcgggtgtg gtcgagcgtg cgcagcgcga tcttcaggcc ctcaccgcgg gagccgatca
     4561 tccggtcgcc cggaatcgtg cagttgtcga accacaactc acgggtcggg ctgcccttga
     4621 tgccgagctt gcgctccttc tcactgaagg tgaagcccgg gtcgctcttc tcgacgacga
     4681 aggccgagat gttggcgccg cggcggccgt cggggtcggt gacggcgagc accgtgtaga
     4741 agtcgttgac acccgcgttg gtgatccagg ccttgtggcc gttgaggatc cacgacccgt
     4801 ccggctgctc ggtcgccgtg caggtcattc ccgacgtgtc cgacccggcc tcgcgctcgg
     4861 acaggccgta acagaacccg ctcttgccct cggccatcgg ggtcaggtag cggtgtttga
     4921 ggtcctcgct cgcggcgagg atgagcggca tactcccgag tttgttgacg gccgggatga
     4981 gggaactgga cgcgcagacc cgcgagatct cctcgatgat gatgcacacg gccaacgcgt
     5041 cggcaccggc gccgccgtac tcctcgggca cgtgcggtgc gaagaagtcg gacgccacga
     5101 gggcgtcgtg agcctcttgc gggtagcggg cgttctcgtc gacatccgcg gcatacggct
     5161 cgatctgcgc ctcggcgagc tcgcgcactg cggcgcgcag ctgacggtgg gagtcggaaa
     5221 gctggaaggc gtcgaagtcc gggttcacgc gccacacgct actccggggt atgccgcgcc
     5281 ggccggcggt gcggcgaggt tcaccctcgt gtgcgaagga acacctcgac cgagctcggc
     5341 gttcggacta gcacgtcacc cgcccgttga cgccacccga aggagcccct gatgcgtgcg
     5401 atctggaacg gaacggtcct ggccgagagt gacgacacgg tggtcgtcga aggcaaccac
     5461 tacttcccga cggcggcgct gcgacacgac ctggtgcggc cgtcggacac ccacaccgtg
     5521 tgcccatgga agggcacggc gagctacttc tcggtggagg tcgacggcca ggtcaacgag
     5581 gacgccgtct ggtactaccc cgaccccaag ccgaaagcgg ccatggtcgc cgaccgggtg
     5641 gccttctgga aaggcgttcg cgttagcgat taaccacgcg tcagcgagtg cccggtcagc
     5701 cgggcagccc gggggacgtc ccggtgagcc cctcgcacag cgaccacagg gtgcgttggg
     5761 cgtcgcgatc gtgcgaggcg gccgacgagc cgacgatccg cggcgggccc gacatctcac
     5821 cgaaccctgc gggcccgaca tacgagcccg ggggcaggtc gggtgccgtt gcggcataga
     5881 ggatcggcag ggcgccctgg tcggccgact gcgccacctt gagcaggctc tccagttgat
     5941 gcaccatcgg ccagctcagg acggcggtat gccgttgcag ccccgtttgc gcgtaaccgg
     6001 ggtgcgcggc atacgagcgg accgtcgagc cggcgagcgt gagccgacgt tgcaactcgt
     6061 aggagagcat gaggtcggcg agtttggacg cggagtaggc ccgccacgcg ttgtagcggt
     6121 ggtggcggaa gctggggtcg gcgaggtcga gcttgccggc tcggtgcatc atcgagctga
     6181 cccagacgac ccggtcggtg atctggtcga gcaacaggct ggtgagcgcg aacggcccaa
     6241 gcacattggt cgcgaactgc aactcgtgcc cgtcgaccga cgcctcctgc ggcaccgcca
     6301 tgatgccggc gttgttgacc aggacgtcga tctggcgccc acccagcgcc tcggcagcct
     6361 cgctgaccga cgccaacgac gacaggtcga tcgtcagcac ctcggtgctt ccggcgatct
     6421 ggcgctgcgc ggccgcaccc ttgtgcgggt cacggcaccc gaggatgacg tgcgcgccgc
     6481 ggtcggccag gacgctggcg gactccaggc cgatgccact gttggcaccg gtgatgagga
     6541 cgacccgccc gctctggtcg gggatctcgt cgacggtcca cgacatgggt tcagcccttc
     6601 aactcgggga tgacggcgcg ctcgaacagg tcgatcgacg tcgtgtcata ggccgcgtcg
     6661 gcgaagtagg cgatggtgta gcccagcccg gcccgacgca gtgcggccag ccgctccaca
     6721 acctgctccg gcgtgccgca gccgaccgag ccgtgcagcc cgtcaatctc cctgcggacc
     6781 gccgcctccg gcaggccgca gacccggatc cggtggtcgg cataccactc gagccggtcg
     6841 ttgagctcga cctcggtctc gccgatgaag acgttgacgt tgatcgagcg gacgatctcg
     6901 gcctcatcgc gcccgagcgc ggcgcagtgg gccttgagga tctcgttctt gcgagtgaac
     6961 tcctcgaggt cgccgaagaa gttggtgtag tcggcatact tcgcggcgat cttcagcgtc
     7021 accttctcgc cgccaccggc cacccacatg ggtatgccgt tcgccgggct ccccggagca
     7081 gcggtgcctt gcagcgggcg cggcgcgcag atggcgccgt cgacggtgta gtacgtgccg
     7141 gcatacgtcg cgctgcccgt ggtccacatc tgccggaaga tctcgacgcc gtcgcgtagg
     7201 gcgccgagcc ggtcgcgggc ctcggggaag ccgtaaccgt aggcgcgcca ctcgtgttcg
     7261 taccagccgg cgccgatgcc catctcaacg cggccaccgc tgatgatgtc gaccgtcgcc
     7321 gcgaccttgg cgaggtaggc ggggttgcgg taggccatgc aggtgcacat ctggcc
//
LOCUS       DAOMZY010000160         7360 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_9494_length_7360_cov_6.837509, whole genome shotgun sequence.
ACCESSION   DAOMZY010000160 DAOMZY010000000
VERSION     DAOMZY010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7360
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9494_length_7360_cov_6.837509"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="PLK64_09910"
     CDS             <1..886
                     /locus_tag="PLK64_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPV80104.1"
                     /translation="SPSQELLRVGLVQHAWDADADRLRARLSAGVHLAVGAGARIVFL
                     PELTLSRYPADTRPSGIPAVNAEDLESGPTVTFASELAREHGVYVHASLYERADAGDG
                     RGYNTAVLVAPSGELVARTRKTHIPVTAGYYEDLYFRPGPADDAYPVVALEDPAVRLG
                     MPTCWDEWFPEVARAYALGGAQVLAYPTAIGSEPDHPEFDTQPLWQHTIIAHAIANGL
                     FVVVPNRTGSEGIITFYGSSFIADPYGRVLVQAPRDSEAVLVADLDLDQGSDWLDLFP
                     FLRTRRPDSYAALIRTQA"
     gene            complement(924..1547)
                     /locus_tag="PLK64_09915"
     CDS             complement(924..1547)
                     /locus_tag="PLK64_09915"
                     /inference="COORDINATES: protein motif:HMM:NF006743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HPV80105.1"
                     /translation="MNLADLADLAVRARDLMAPGRRVVLGIVGPPGVGKSTLAQALVR
                     EVGERAAYLPMDGFHLPQARLRALGTRDRMGAPETFDAAGYADLVDAVVASPGMPLTA
                     PGFDRTVEEPVADGVRIDASARLVVTEGNYLLLPGGDWERARVRMAAVWQLRLADETR
                     RERLLARHIAFGRPPAEALEWMTRVDEPNARLVQSYAARADLIIDVT"
     gene            1784..3205
                     /locus_tag="PLK64_09920"
     CDS             1784..3205
                     /locus_tag="PLK64_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80106.1"
                     /translation="MTGRPTLVELCRAAVVGPAWDHRIRRGGGAKASHVALHLGARLV
                     QANWPAHAGTPPRPREPLTTAQADAARATLDRVVPLISEALDAHQRIVVATLEILDDK
                     GAPTTAAVLSEFCSVRVGQHYATSIGQPLDEADVARKLAERHLDRPEPMAAEEFFQRH
                     HTAYLRLWNQDGKEAIRTHPPSRIGLGHDERPPMIGVPGERSWQLGTDRSVYDRYRLH
                     FSDAAKLRTRRPGREGRAVEDPEHLAQLEIWRACQGYGLARPAHRVILESLARATLDE
                     STPLAQARAIWQRWCATPRRIAEGGPGTRDDDLTAAISAKVRVWAADQIAWSLRKSYG
                     ELSSMDDHVVRAIVQKVWMGLSAVEREEVGVVDSTLINGWVSTALDRGVAQVLHERAR
                     AIDTPPSARRCHRTCELLTERPDLARVLVNPDSDDLARQGACTAYEAAAGERWGTAWR
                     EQALTGTEAAAYVSGLTPLEEVG"
     gene            3202..4668
                     /locus_tag="PLK64_09925"
     CDS             3202..4668
                     /locus_tag="PLK64_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80107.1"
                     /translation="MSTAASDHVTVNRLGDTFVVGEDPGAAAGLAVLPVKGGIFVVDP
                     INPRGPGQLRLQGPFDLELVETLFGTAVAAAVDVRLDPPERAVVAMAQSHERRILGRL
                     GALRWLQRASPLALDDRLLRVEGLLLEASIADVLDVDEGSESLHDWAPSLVAWAHRCV
                     SPDLREPVSPQFLDLVADGLAEDSAGSPWSPESADHSGQDAEARAELAAYLRAGLARA
                     RADAPDAPGLPCAPIDALRPQTRAATTSSRNGQLHGSATVDWARVPRGLTLQIEDGAS
                     WRVDTTPGNAHVSVVVTAPTADPTLRLLDVAGQPGRSGVTPALQATLTHPEWMLPLAV
                     VELTLRPETDCWLGVEALGPAVLELIADLDPTAFHMDLHVPGAGRRPRNGAAASIDQA
                     TRWAVRGVASVRLAAAYRAESRAEIHRGACDAFALAGSLFKASADPRDQLAAEQCRAA
                     VRLLGRPHAHSHGPEVIDPEPELTVAERWYAAHLAAAH"
     gene            4831..>5312
                     /locus_tag="PLK64_09930"
     CDS             4831..>5312
                     /locus_tag="PLK64_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80108.1"
                     /translation="MSMQVLDPELASVVWWGTHTLRVRAHAARGASLCPTRFAPYGHA
                     DPLAQQANLRCWEDGCMGIVHSIYAATSDRLARRAGSDRQAVADRMRYARTVVASQVA
                     ELDRGRRVARGLPARPTRNDGIAGRINEALDAAAAGPTEARWLVQLFRMIRGYVCRDN
                     R"
     assembly_gap    5313..5412
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5616..6566)
                     /locus_tag="PLK64_09935"
     CDS             complement(5616..6566)
                     /locus_tag="PLK64_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80109.1"
                     /translation="MDRVARIAADLDSFHRGRGIHADDFATRVGPHLAQVVFADSDPE
                     PTYGRDLLKRWFLVASQDLPPDLRAVFLAASGLTQPGPKLTDRLGALSLSTGLASRTL
                     VRRLRQADVLVAAKLDSLSYVSEDDNPFARRGWYVERLESTAYLNEERPRFVGVREIL
                     CTLDGLEQICESLSVPRPPGDTSPVDLIVEATEGCEVDTLTRVSPSTWQVGLALPKPL
                     TVGQRHRLGVSIAMPSRAFVKPYNAFVPVRRTRSFAAVVHLPNDGSVVGAWRLDGVPP
                     TAMEDGVPIGDSFDLSAPEPLAAEWTTVRRGLGYGIGWSW"
     gene            complement(7021..>7360)
                     /locus_tag="PLK64_09940"
     CDS             complement(7021..>7360)
                     /locus_tag="PLK64_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HPV80110.1"
                     /translation="ARDDINALYAVERVSAALTEPPSSVVMDTAAWDAALGDYWAEHD
                     EVVLDADARGPAYLSIGPHPDGGRRWAVRQTIADPAGDRDWVIEADVDLDASDAVGEL
                     VLTTTALRRL"
BASE COUNT         1107 a         2586 c         2519 g         1048 t
ORIGIN      
        1 ctctccgagc caagaactgc ttcgcgtcgg cctcgtccag cacgcgtggg atgccgatgc
       61 ggaccgcctt cgcgccaggc tcagtgctgg cgttcacctc gcggtgggcg cgggcgcacg
      121 gatcgtcttc ctccccgagc tgaccctctc gcgttacccc gccgacaccc gcccgagcgg
      181 cataccagcc gtgaacgccg aagacctcga gtccgggccg acggtgacct tcgcgtccga
      241 gctggcccgc gagcacggcg tgtacgtcca cgcgagcctc tacgaacgcg ccgacgccgg
      301 cgatggccgt ggctacaaca ctgccgtcct cgttgctccg tccggggagt tggtggcccg
      361 cacccgcaag acccacatcc ccgtcaccgc cggctactac gaggacctgt acttccggcc
      421 gggaccggca gatgacgcct atcccgtggt ggcgcttgag gatcccgccg tccggctggg
      481 gatgccgacc tgctgggacg agtggttccc ggaggtggcg cgggcctacg cactcggcgg
      541 cgcccaagtc ctcgcctacc cgacggcgat tgggtcagag cccgatcacc ccgagttcga
      601 cacgcagccg ttgtggcagc acacgatcat cgcccacgcg attgccaacg ggttgttcgt
      661 cgtcgtgccc aaccgcaccg gcagcgaggg gatcatcacc ttctacggga gttcgttcat
      721 cgccgatccg tatgggcggg tcctcgtcca agcaccccgc gacagcgagg ccgtcctggt
      781 cgccgacctc gatctcgacc aaggctcaga ctggttggac ctgttcccct tcctacgcac
      841 ccgccgcccc gactcctacg ccgccctgat ccgtacccag gcctgatccg tacccagcct
      901 gatctgcgag ggggctggac ccctcaggtg acgtcgatga tgaggtcggc gcgggcggca
      961 tacgactgca cgagccgagc gttcggctcg tcgacgcgcg tcatccactc cagcgcctcg
     1021 gcgggcggcc tgccgaaggc gatgtgccgc gccagcagcc gctcccgccg agtctcatcg
     1081 gccagtcgca actgccagac cgccgccatc cgcacccggg cccgctccca gtcgccgccg
     1141 ggaagcagca ggtagtttcc ctcggtcacc accagccgcg cactggcatc gatccgcact
     1201 ccgtcggcga ccggctcctc gacggtgcgg tcgaaaccgg gggcggtgag cggcataccg
     1261 gggctggcga ccaccgcgtc gaccaggtcc gcgtaacccg cggcatcgaa cgtctcaggc
     1321 gcccccatcc ggtcgcgtgt gcccaaggcg cgcaacctcg cctggggcag gtggaaccca
     1381 tccatcggca ggtatgccgc ccgctcaccg acctcgcgga cgagggcttg ggcgagggtc
     1441 gacttcccca cgcccggtgg gccgacgatg ccgagcacga cccggcgacc tggggccatg
     1501 agatcacgcg cccggacagc gagatcggcg agatcggcga ggttcaccct cgcgagagta
     1561 gacggccgac cggtttgtcc ggagcgcccc ccttctttct gtccgtcagg gatggcatgg
     1621 cgggtgaggt cacggcaccc gacatcggaa catcaacacg gtcgggtgag catgcagggg
     1681 ggctcatccg gccgtgagcc acatcggcgt gacgaccggc caggggaggc gtgctgccga
     1741 tgagggttga tgcgcatgct cgcgaggttc tcgggagtcc ggggtgaccg gccgcccgac
     1801 gctcgtcgag ctgtgccggg ctgccgtcgt cggcccggcc tgggaccatc ggatccgccg
     1861 cggcgggggt gccaaggcga gccatgtcgc tctgcacctc ggcgcccgac tcgtccaggc
     1921 caactggccg gcccacgcgg ggactccgcc acgtccgcgc gaaccgctga cgaccgctca
     1981 ggccgacgcc gccagggcga cgctcgatcg ggtcgttccc ctcatctccg aggccctgga
     2041 cgcccatcaa cggattgtgg tcgcgaccct ggagatcctc gacgacaagg gcgcgccgac
     2101 caccgccgcc gtgctcagcg agttctgctc ggtccgggtc gggcagcact acgcgacctc
     2161 gatcgggcag ccgctggacg aggccgacgt cgcacgtaag ctcgccgaac gccacctcga
     2221 tcggcccgag ccgatggctg cggaggagtt cttccaacgg caccacacgg catacctgcg
     2281 gctgtggaac caggacggga aggaggcgat ccgcacccat ccgccgtcgc gcattggcct
     2341 cggccacgac gagcggcctc cgatgatcgg cgttcctggg gaacgctcct ggcaactcgg
     2401 gaccgaccga tccgtgtatg accgctaccg gctgcacttc agcgacgccg ccaagttgcg
     2461 gacccgtcga ccgggccggg agggacgggc ggtcgaggat cccgagcacc tggcccaact
     2521 cgagatctgg cgcgcgtgtc agggttacgg gctggcccgg cccgcccacc gggtgatcct
     2581 cgagagtttg gccagggcca ccctcgatga gtccaccccg ctggcgcagg cacgtgcgat
     2641 ctggcagcgc tggtgcgcca cgccgcgtcg gatcgccgag ggtggcccgg gcacccgcga
     2701 cgacgacctc accgcggcga tctcggccaa ggtgcgggtg tgggccgcag atcagatcgc
     2761 gtggtcgctg cgaaagtctt acggtgagct atcgagcatg gacgaccacg tcgtgcgcgc
     2821 catcgtgcaa aaggtatgga tggggctgtc ggccgtcgag cgagaggagg ttggggtcgt
     2881 ggacagcacg ttgatcaacg gttgggtgtc caccgccctc gaccgggggg tcgcgcaggt
     2941 gcttcacgaa cgggcgcgag ccatcgacac gccgccgagc gcgcgacgct gccatcggac
     3001 gtgtgagttg ctcaccgagc gacccgacct ggctcgcgtc ctggtcaacc cggactccga
     3061 cgaccttgcc cgccaggggg cgtgcacggc atacgaagcg gcggccggtg agcggtgggg
     3121 gaccgcgtgg agggagcagg ccctgaccgg gaccgaagcg gccgcctacg tgtccgggct
     3181 caccccgctg gaggaggtcg ggtgagcaca gccgcgtccg atcacgtcac cgtgaatcgg
     3241 ctgggcgaca cgttcgtggt tggtgaggac cccggcgccg ccgccgggct ggccgttctg
     3301 cccgtcaagg gcggcatctt cgtggtcgac ccgatcaacc ctcgcgggcc gggacagctg
     3361 aggcttcagg gcccgttcga cctggagctt gtcgagaccc tgttcgggac cgccgtggcc
     3421 gcggctgtcg acgtgcgcct cgacccgccc gagcgggccg tcgtggcgat ggcccaatcc
     3481 cacgaacgcc ggatcctcgg tcgcctcggg gcgttgcgct ggctgcagcg ggcctcgccg
     3541 ctggcgttgg acgatcgcct gttgcgggtc gaagggctgc tcctggaagc gtcgatcgcc
     3601 gatgtgctcg atgtggacga ggggtccgag agcctgcacg actgggcgcc ctcgctcgtg
     3661 gcgtgggcgc atcggtgcgt gtcgccggac ttgcgcgagc ccgtgagccc gcagttcctc
     3721 gacttggtcg ccgacgggct cgccgaggac tcggccgggt cgccctggtc gcccgaatcg
     3781 gcagaccaca gcggccagga cgccgaggcg agagctgagc tcgcggcata cctgcgcgcg
     3841 ggactggcgc gagcccgagc cgacgcaccc gacgcacccg gcctgccgtg cgctccgatc
     3901 gacgcgctgc gcccgcagac gcgcgctgcg accacaagct cccgtaacgg gcaactgcac
     3961 gggtccgcga ccgtcgactg ggcgcgggtg ccgcgggggc tgaccctgca gatcgaggac
     4021 ggcgcctcct ggcgggtcga cacgactccc ggcaatgccc acgtatcggt ggtcgtcacc
     4081 gcgccgactg cggacccgac cttgcgactg ctcgacgtcg ccggtcaacc gggtcgatcc
     4141 ggcgtgaccc cagccttgca ggccaccctg acccaccccg agtggatgct tcccctggcc
     4201 gtcgtcgagc tcaccctgcg ccccgagacc gactgctggc tgggggtcga ggcgctcggc
     4261 ccagcggtgc tcgagctcat cgccgatctg gaccccaccg ccttccacat ggaccttcac
     4321 gtgcccggag caggccggcg accccgcaac ggcgccgcag cgagcatcga tcaggccact
     4381 cggtgggccg tccgcggcgt ggcctcggtg cgtctcgccg cggcataccg ggcagagagc
     4441 cgagccgaga tccaccgtgg cgcctgcgac gcgttcgccc tggccgggtc actgttcaag
     4501 gcctccgccg accctcgcga ccaactcgcc gcggagcaat gccgcgccgc ggtccgcctc
     4561 ctcggcagac cccacgcgca ctcgcacggc cccgaggtca tcgaccccga gcccgagctc
     4621 accgtcgccg aacgctggta tgccgcgcac ctcgcggccg cgcactgagg ggctgacgcc
     4681 ggccaggggg tgggccggga gttggaccgg acaattaccg gacagtatcg gcccacaacc
     4741 ttcaccttgt ggacccctga caacacgatg acagggccat gccgaccacc gcgtcgacat
     4801 gcccggccca cgtgttttcg gaggtttccc gtgtcgatgc aggtcctgga ccctgagttg
     4861 gcgagcgttg tgtggtgggg cacgcacacg cttcgggtgc gtgcccatgc cgcgcgcggc
     4921 gcaagcctct gtccgacccg gttcgccccc tacggtcacg ccgacccgtt ggcgcagcag
     4981 gccaacctgc gctgctggga agacggctgc atgggcatcg tgcacagcat ctatgcggcg
     5041 accagtgacc ggctggcccg gcgggccggg tcggatcgtc aggccgttgc cgatcgaatg
     5101 cgctacgccc gcaccgtcgt cgcatcacag gtggccgagt tggaccgggg ccgcagggtt
     5161 gctcgtgggc tgccggctcg accgacccgc aacgacggga tcgccggccg gatcaacgag
     5221 gccctcgacg cggcggccgc gggcccgacc gaggcacgct ggctggtgca gttgttccgg
     5281 atgattcgcg gctacgtctg ccgcgacaac cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnctcctgcg acagttcatg acgaggtatg ccgcgtaccg ccgccgcggg
     5461 cagcacccgg caggcgcctt ccggacggcg tcactcgagg tcttcggtca ggagccgtcc
     5521 ggtgatctgc gcgacgtcat cgtcgatcta gagtcgggct tggtgggctg acgggctggc
     5581 tgtcgggctg actgggagtc ggcgaggggg tccgcctacc aggaccagcc gatgccgtag
     5641 cccaacccac gacggacggt ggtccattcg gcggccaggg gctccggcgc ggacaggtcg
     5701 aaggagtccc cgatcggcac gccgtcctcc atcgccgtcg gcgggacacc gtccaggcgc
     5761 caggcaccga cgaccgaccc gtcgttcggc aggtggacga ccgccgcgaa actgcgagtt
     5821 cgtcgcaccg gcacgaacgc gttgtagggc ttgacgaaag cccgtgacgg catcgcgatc
     5881 gagacgccga gacggtggcg ttgcccgacc gtcagcggct tcggcagcgc cagacccacc
     5941 tgccacgtgc tcgggctgac ccgggtcaac gtgtccacct cgcacccctc cgtcgcctcc
     6001 acgatgaggt cgacgggtga cgtgtcaccg ggagggcggg gcacgctcag cgactcgcag
     6061 atctgctcga gcccgtcgag cgtgcacaag atctcgcgca cgccgacaaa tcgcggccgt
     6121 tcctcgttga ggtatgccgt ggactccagc cgctcgacat accaccctcg ccgcgcgaag
     6181 ggattgtcgt cctcggagac gtagctcaac gaatcgagtt tggcggcgac gagcacatcg
     6241 gcctggcgca gccgacgcac cagggtgcgg gacgcgagac cggtgctcag ggaaagcgcg
     6301 cccaggcgat cggtcagttt gggcccgggc tgggtcaacc cgctggccgc caggaagacc
     6361 gcgcggaggt ccggtggcag gtcctggctg gcgacgagga accagcgctt gagcaggtca
     6421 cggccatagg tcggttcggg gtccgagtcg gcgaagacca cctgtgcgag atgcggaccc
     6481 accctggttg cgaagtcgtc cgcatggatt ccgcgcccac ggtggaagct gtccagatcg
     6541 gcagcaatgc gtgcgacccg atccacctcg ttctcccttg gccggtccgt gtcagccagc
     6601 tcccgtcgac cgacgaggcg aaccataccc ggacggaggc cacggcttga acctgcgctc
     6661 gaaagtgaac agggcgccgg gtgcgaattc acacccggcg cctgcggtcg ccgtgggtca
     6721 gcaacccgcg aacatgacga agcttgtgag aacggaccag aagtcgtcct tgatggagca
     6781 atgccactgc atcgcaaccc ctcctcccct gcgtgtcgag cggcgcggac gcccgacaac
     6841 cgatcccgat tggggatggg caggtgagag gagggggtcg acgaaatagg cccgcccgaa
     6901 tggcgggccc agcgcgtgcg acggcaaagg cgagagggaa ctaaagccag aaatgcgtcg
     6961 aaaaaggacc ctccggcacc gaagaccggt caggcgaagc ggggggctgg cctggctccc
     7021 ttagaggcgg cgcagggcag tggtcgtgag gacgagctcg cccacggcgt cactcgcgtc
     7081 caggtcgacg tcagcctcaa tgacccagtc gcggtcaccg gccgggtcgg cgatggtctg
     7141 ccggaccgcc caccggcgcc cgccgtcggg gtgcggcccg atcgagaggt atgccggtcc
     7201 gcgggcgtcc gcgtccagca ccacctcgtc gtgctcggcc cagtagtccc ccagcgccgc
     7261 gtcccaagcc gcggtatcca tgacgaccga actcggcggt tcggtgaggg cggcggagac
     7321 gcgctccacg gcatacaggg cgttgatgtc gtcgcgggca
//
LOCUS       DAOMZY010000161         7320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_9558_length_7320_cov_5.847213, whole genome shotgun sequence.
ACCESSION   DAOMZY010000161 DAOMZY010000000
VERSION     DAOMZY010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7320
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9558_length_7320_cov_5.847213"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            614..2785
                     /locus_tag="PLK64_09945"
     CDS             614..2785
                     /locus_tag="PLK64_09945"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPV80111.1"
                     /translation="MPTQSLRTHTEVLHLVRRLEEAEAEAALAVKSAFATVRSAMVSR
                     ALADIPLHRIRETVPDGRLRLGALERAGMTTVQDVLAYPYGLDDVAGVGEQTAHQVYA
                     AADRLRRAVEDDLAFRVDLDPSDGNTTRLIRNLCHWSLSALALRRLSPAIDAARSALI
                     PLRALPKRPDAFVGFFGLDPESDQSVRRELDSWSGWAARHAGFVEEARAGLAPRPTPD
                     EAWADFRRRAADYYAWLGEIVGLTLDDDVQAGQVPDEVADAVRRLSLDTSLVAASLRG
                     YQEFGAKYALVQRRTIIGDEMGLGKTVQAIAAIAHAQAQGDAHSLIVCPASVLINWLR
                     EVPKHSRLRVHRLHGSDRHSGLVAWRRYGGVGVTTYGQLDTLRFQPSDRLAFLVADEA
                     HYLKNQYTRRGTAVASVIPRADRVLFLTGTPLENRLEEFQSLVQYVQPDLTRTLNMSD
                     AIASARAFRDRIAPVYLRRNQADVLTELPPRIDVDDWVELTPADRRAHDRAVLQGNFM
                     ALRRAAFLGDGAQSAKVERLVELVAEAVETGRKIVVFSFFRDVLDEAAGALAEVAPVI
                     GPLTGSTRPEDRQGLIDRFTDLQVPAVLVSQVEAGGVGTNIQAASVVILCEPQVKPST
                     ELQAVARVHRMGQVERVQVHRLLGSDSVDQRMLWILAEKQRAFDLYVRDSAAAEAAPE
                     AVDISERELAMRVIAEEQQLLAARLTRGPVQHEGSSGRSAE"
     gene            2827..3531
                     /locus_tag="PLK64_09950"
     CDS             2827..3531
                     /locus_tag="PLK64_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPV80112.1"
                     /translation="MVKTLPKPTRAPALAPEARRAALVDAAVPLVLERGFDVTTKDLA
                     AAAGVAEGTIFRVFESKDDLVLAAARSVFARADHLDELAAISLEWPLEARLAAAVRIW
                     QTVARRMVSVFVAFHGAGDHQRLGNPHDLVDPTIVARAEAIIAALLAPEADRLRRPIP
                     DIVRIMGSLVLASVHPVDALSPASTPEDLVDLLLHGVLADAPTPHVSDPDETPLTVPL
                     REGPASCSAHAAYLDR"
     gene            3544..5280
                     /locus_tag="PLK64_09955"
     CDS             3544..5280
                     /locus_tag="PLK64_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV80113.1"
                     /translation="MLWRLIRPRLAPHGTAIVIIVVLQLASTIASLYLPNLNGDIIDQ
                     GIAKGDAGYILRTGGWMLGISVLQIAASVYAVYLAARTSMAFGRDTRAQVFHGVVDFS
                     AREVSQFGAPTLISRTTNDVQQAQMVVFMTLSMMVGAPIMMIGGVIMALRTDVGLSWL
                     VAVSVPLLALLVGLVLRQMLPRFRVMQDSIDSVNRILREQITGIRVVRAFVREPHEKA
                     RFAEANGTLTGASLGVGNLMALVFPLVMLVFNASTVAVLWFGAQRVDAGDMQVGALTA
                     FMSYLMQILMSVMMATMMSMMIPRAAVSAERIMKVLDTIPSVHPPVNPVTDIASPAAV
                     VFESVEFRYPGAEHSVLQDISFEARPGETTAIIGSTGAGKTTLIGLIPRLVDVTGGRL
                     LVGGVDVRDADPDTLWSRIGLIPQKPYLFSGTVASTLRYGNPDATDEELWEALRIAQA
                     EDFVRAMPEGLAAPIAQGGTNVSGGQRQRLAIARALVRRPEVYLFDDAFSALDVATDA
                     RLRAALAPVTREATVIVVAQRVSTIIDAEQIIVLEDGLVVGRGRHTELLESCPTYQEI
                     VVSQRAAEEVAA"
     gene            5277..7298
                     /locus_tag="PLK64_09960"
     CDS             5277..7298
                     /locus_tag="PLK64_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV80114.1"
                     /translation="MSEGVKTAEEKAAEARRGPRHSGPPHMAMGMPAEKSMSFGPSAR
                     RLLSWLAPERVKVTTVLVLVVLSVVLNSVGPKILGRATDLIFAGLVGRMLGTNIPAGT
                     SRADVIAAMRAQGQDRMADMLQGVDFIPGQGIDFAAIGQVLLVVLAIYVVASVLSWLG
                     TRLLVGVVQRAMLRMREQVQAKLDRLPLAYFDNQPRGELLSRVTNDIDNVSTSLQQTM
                     SQLLNSLLTVVAMLAMMFWISWLLALIALVTIPISMVVAAAIGKRSQKLFVKQWAATG
                     QLNSIIEETFTGHQLVKVFGRQREVQEQFDGKNGELYDSAFGAQFISGIIMPVMMFVG
                     NLTYVAIAVVGGLKVASGQMTLGDVQAFIQYSRQFTQPLTQVASMANLLQSGVASAER
                     VFEVLDAPEQEAEAAYPATIAEPHGEVRFEDVSFAYTVDRPLIEHLDLDVKPGHTVAI
                     VGPTGAGKTTLVNLIMRFYELDGGRITLDGVDIRDLSRQGLRERIGMVLQDTWLFAGT
                     IRDNIAYGRPGATEEEVLAASRATFVDRFVHSLPDGYDTVLDAEASNLSAGERQLVTI
                     ARAFLSEPSLLILDEATSSVDTRTELLVQKAMRALRSDRTSFVIAHRLSTIRDADVIL
                     VMEAGRIVEQGTHEELLAQHGAYARLYAAQFSGAAAEAADAAAEAAVGA"
BASE COUNT         1128 a         2455 c         2412 g         1325 t
ORIGIN      
        1 ccttgcgctt tactcatgcg gcgttcgagg aggagtgagc accgtctcgg cacccccttt
       61 tgctaacgaa cgcgaggagg ccatctggct cacaggccgg ctcactgatc tcgttgcctg
      121 ggcgaagtgg ctgatcaacg ctgcagatcg ggcgctgccc gagctgcagc ggaacctgca
      181 acggcatgtc acggcgcttc cccacgtcag cccgggcgca ttcgcggtca ccttgagcaa
      241 ggaagatctg gctgtcttcg tcggggtaac tcgggtgggg gacgtcggcc ggcttgccgt
      301 cgacctcgaa ggcgcccgat tggtccttcg tgacgtcaat caagctctag cagcgatcga
      361 gcctgccact cggcggtacg ttctctcact gcgttccaca tcgatccacg ccggcgcccg
      421 agccgcgctc ccatgggctc gttcctggga ggagtgggcc agccgaaaag ggggtctcgc
      481 gcgccttcag tgggcggccc accgactggg gggcgaacga tctgaagggg gaacagcgtt
      541 cggaccggaa tggctcgcat caagcggacc gacgttcgcg gccgccgtcc gcggccccgc
      601 tccaggtgcc tgggtgccta cccagtcatt gcgaacccac acagaggtcc tgcatctggt
      661 gcgtcgcctg gaggaggcgg aggccgaggc agcgttagca gtcaagtcgg ctttcgccac
      721 tgtgcgatcc gcgatggtga gtcgggcgct cgccgacatc cccctccaca ggattcgtga
      781 gaccgttccc gacggtcggc tgcggctggg cgccctggaa cgtgcgggca tgaccacagt
      841 tcaagatgtc ctcgcctatc cctacggact tgacgatgtg gccggtgtcg gcgagcaaac
      901 ggcacaccag gtgtatgccg ctgccgatcg gctacgtcgg gccgttgaag acgatctcgc
      961 cttccgcgta gacctggatc cctcggacgg caacaccact cggcttatcc gcaacctgtg
     1021 tcactggtcc ctgtcggcgt tggcgcttcg tcggctgagt ccggcgatcg acgcggcacg
     1081 ttccgcgctg atcccgctcc gagcgttgcc gaaaaggccg gatgccttcg tcgggttctt
     1141 tgggcttgat cctgagagtg accagtccgt gcgccgtgaa ctggattcat ggtcaggctg
     1201 ggcggctcgg cacgccggtt ttgtcgagga agcgcgcgct gggttggcgc cacgtccaac
     1261 gcccgacgag gcctgggcgg acttccggag gcgcgcggct gactattacg cctggttggg
     1321 cgagatcgtc gggctcacgc tggacgacga cgtccaggcg ggccaggtgc cggacgaggt
     1381 cgccgacgcc gtgaggcggc tctcgctgga cacgagcctg gtcgctgcct ccttgcgggg
     1441 gtatcaggag ttcggggcca aatacgcact ggtccaacga cgtacgatca tcggcgatga
     1501 gatgggcctg gggaagaccg ttcaggcgat cgcggctatc gcgcacgcac aagcgcaagg
     1561 ggacgcgcat tcactcatcg tttgcccggc cagcgtgctg atcaactggc tccgggaggt
     1621 ccccaagcac agcaggctgc gggtgcatcg acttcatgga agcgacaggc actcgggcct
     1681 ggttgcctgg cggcgttacg gaggcgtcgg tgtgacgaca tacgggcaac tggacaccct
     1741 tcggttccag ccttccgatc gcctagcatt cctcgtcgcg gatgaggcgc actatctcaa
     1801 gaaccagtac acgcggcgtg gaacggccgt tgcaagcgtg atcccacggg ccgatcgggt
     1861 gctgttcctt accgggactc ctctggagaa tcgcttggag gagttccagt ccttggtcca
     1921 gtatgttcaa ccagatctca cgcggacgtt gaacatgtcc gacgccatcg ctagcgcgcg
     1981 ggcgttccgg gatcgaatcg caccggtcta tcttcgccga aaccaggcgg acgttctcac
     2041 cgagctcccg cccaggatcg acgtcgatga ctgggtcgag ctcaccccgg cggacaggcg
     2101 agcacatgac cgcgccgtcc tccagggcaa tttcatggcg cttcgtcgag ctgccttcct
     2161 gggtgacggg gcgcagagcg cgaaggttga gcgattggtc gaactggtcg cagaggcggt
     2221 cgaaacggga cgcaagatcg tcgtcttctc cttcttccga gacgtcctcg atgaagcggc
     2281 gggcgctctc gccgaggtag cgccggtcat cgggccgttg accgggtcga ccagacccga
     2341 ggatcgccaa gggctcatcg accggttcac cgaccttcaa gttcctgccg tcctcgtgag
     2401 ccaggtcgag gctggtggag tcggaacgaa catccaagca gcctctgtgg tcatcctgtg
     2461 tgagccccag gtcaagccga gcacggaact tcaggccgtc gctcgtgtcc accggatggg
     2521 gcaggtcgaa cgggttcagg tccaccgttt gttgggctca gacagtgtgg atcagcgaat
     2581 gctgtggatc ctcgctgaga agcagcgggc cttcgatctg tatgtccgcg acagcgccgc
     2641 agctgaagcg gcgcccgagg cggttgacat ttcggagcgg gagttggcca tgcgggtcat
     2701 tgccgaggag cagcagttgc tggctgctcg ccttactcgg ggtcccgttc aacacgaagg
     2761 gtcctccggc cgcagcgcgg agtgaacgag cttgtgagtg agcactcact cacttatagt
     2821 cccgctgtgg tgaaaaccct gcccaagccg acccgagccc cggcgctggc ccccgaggct
     2881 cgccgggccg cgctcgtcga cgcagccgtg ccgctcgtcc tcgagcgtgg tttcgacgtg
     2941 accacgaagg atctcgccgc ggccgccggg gttgccgagg ggaccatctt ccgggtcttc
     3001 gagagcaagg acgacctcgt cctcgctgct gccagaagcg ttttcgcccg cgctgaccat
     3061 ctcgatgagc tcgcagccat cagcctcgag tggccgctgg aggctcgtct ggcggcggcc
     3121 gtccggatct ggcagacggt cgcccgacga atggtgtcgg tgttcgtcgc cttccacggg
     3181 gccggtgacc accagcgtct cggcaatccg cacgacctcg tcgacccgac catcgtcgcc
     3241 cgggccgagg cgatcatcgc tgcgctgctg gccccagagg ccgaccggct acggcgcccc
     3301 atccccgaca tcgtgcggat catgggctcg ctcgtgctgg ccagcgtcca cccggtcgac
     3361 gcgctcagcc ccgcctccac gcctgaggac ctcgtcgacc tcctgctgca cggtgtcctc
     3421 gccgacgccc cgacgcccca cgtctcggac cccgacgaaa cccccttgac cgtcccccta
     3481 cgtgagggcc cagcatcgtg ctcggctcac gcggcatacc tcgaccgata ggaacctgac
     3541 ccgatgctct ggcgactcat ccgccctcgc ctcgcgcccc acggcactgc gatcgtcatc
     3601 atcgtcgtgc tgcagttggc cagcaccatc gccagcctct acctgcccaa cctcaacggc
     3661 gacatcatcg accagggcat cgccaagggc gacgcgggtt acatcctgcg cacgggcggc
     3721 tggatgctcg gcatcagcgt gctgcagatc gcggccagtg tgtatgccgt ctacctcgcc
     3781 gcgcgcacgt cgatggcctt cggtcgggac acgcgggccc aggtgttcca cggtgtcgtc
     3841 gacttctcgg cccgcgaggt ctcgcagttc ggtgccccga cgctcatcag ccgcacgacc
     3901 aacgacgtgc agcaggccca aatggtcgtc tttatgaccc tgtcgatgat ggtgggggct
     3961 ccgatcatga tgatcggcgg cgtcatcatg gcgctgcgca ccgacgtcgg gctctcctgg
     4021 ctggtggccg tctcggtgcc gctgctggcg ctgctcgtcg ggctagttct gcgtcagatg
     4081 ctgccgcggt tccgagtcat gcaggactcg atcgactcgg tgaaccgcat tctgcgcgaa
     4141 cagatcacgg gcattcgtgt cgtgcgggcc ttcgtccgcg agccgcacga gaaggcgcgc
     4201 ttcgccgagg ccaacggcac gctcaccggc gcatcgctcg gggtcggcaa cctgatggcc
     4261 ctcgtcttcc cgctcgtcat gctcgtcttc aacgcctcga ccgtcgcagt gctgtggttc
     4321 ggtgctcagc gcgtcgacgc cggtgacatg caggtcggcg cactgacggc gttcatgtcc
     4381 tacctcatgc agatcctcat gagcgtgatg atggcgacga tgatgtcgat gatgatcccc
     4441 cgggccgcgg tgtcggccga gcggatcatg aaggtcctcg acaccattcc ttcggtccac
     4501 cctccggtca acccggtgac cgacatcgcg tcgcctgcgg cggtggtctt cgagagcgtc
     4561 gagttccgct accccggtgc ggaacactct gtgctgcaag acatctcgtt cgaggcccga
     4621 ccgggtgaga ccactgcgat catcgggtcg acgggtgcgg ggaagaccac gctcatcggt
     4681 ctcattcccc gcctcgtgga cgttaccggc ggtcggctgc tcgtcggcgg cgtcgatgtg
     4741 cgagacgccg atccggacac cctgtggtca cgcatcggcc tgatcccgca gaagccgtac
     4801 ctcttctccg gcaccgtcgc ctccaccttg cgttacggca accccgacgc caccgacgag
     4861 gagctctggg aggcactgcg catcgctcag gccgaagact tcgttcgcgc catgcccgag
     4921 ggtctggccg cgccgatcgc ccagggcggc accaatgtct ccggcgggca gcggcagcgc
     4981 ctggccatcg cccgggccct tgtgcgtcgt cccgaggtct acctcttcga cgacgccttc
     5041 tcggcccttg acgtcgcgac cgacgcccgg ctgcgggcgg ccctcgcgcc ggtgacgcgg
     5101 gaagccaccg tcatcgtcgt cgcccagcgc gtgtcgacca tcatcgacgc cgagcagatc
     5161 atcgtccttg aggacgggct ggtcgtcggg cgcgggcggc ataccgaatt gctcgagtcg
     5221 tgtcccactt accaggagat tgtcgtgtcc cagcgggctg ccgaggaggt ggcggcatga
     5281 gtgagggtgt gaagaccgcc gaagagaagg cggccgaggc ccggcgcggg ccgcgccaca
     5341 gcggtcctcc gcacatggcc atgggtatgc cggccgagaa gtccatgtcg ttcggcccct
     5401 ccgcgcgtcg actgttgtcg tggttggcgc ccgaacgcgt caaggtgacc accgtcctcg
     5461 tgctggtcgt gctgtcggtg gtgctcaact cggtcggccc gaagatcctc ggccgggcga
     5521 ccgacctcat cttcgccggg ctcgtcgggc ggatgctcgg caccaacatc ccggccggga
     5581 cgtcccgtgc ggacgtcatc gcggcgatgc gtgcgcaggg tcaggaccgg atggccgaca
     5641 tgctccaagg tgtcgacttc atcccaggtc aagggatcga cttcgccgcc atcgggcagg
     5701 tgctgctcgt cgtgctggcg atctatgtcg tcgcctccgt gctgtcctgg ctggggaccc
     5761 ggctgctcgt cggcgtcgtc cagcgcgcga tgcttcggat gcgtgagcag gtgcaggcca
     5821 agctggaccg tctgcccttg gcctacttcg acaatcagcc gcgtggcgag ttactcagcc
     5881 gcgtgaccaa cgacatcgac aacgtgtcaa cgtcgttgca gcagaccatg tctcagctgc
     5941 tcaactcgct gctcacggtg gtggcgatgc tcgccatgat gttctggatc tcgtggctgc
     6001 tggccctcat cgccctggtc accatcccca tctcgatggt cgtcgctgcg gcgatcggca
     6061 agcgctcgca gaagttgttc gtcaagcagt gggcggcgac tggccaactc aactcgatca
     6121 tcgaggaaac cttcaccggg caccagctcg tcaaggtgtt cggtcgtcag cgcgaggtgc
     6181 aggagcagtt cgacggcaag aacggcgagt tgtacgactc ggcgttcggg gcccagttca
     6241 tcagcgggat catcatgccg gtgatgatgt tcgtcgggaa cctcacctat gtcgccatcg
     6301 ccgtcgtcgg cggcctcaag gtcgccagcg gtcagatgac cctcggtgat gtccaggcct
     6361 tcatccagta ctcccggcag ttcacccagc ccctgaccca ggtggcgtcg atggccaacc
     6421 tcctgcagtc cggcgtggcc tcggccgagc gggtgttcga ggtccttgac gcccccgagc
     6481 aggaggccga ggcggcatac ccggcgacga tcgccgagcc ccacggggag gtgcggttcg
     6541 aggacgtctc gttcgcctac accgtggacc ggccgctcat cgagcacctc gatctcgacg
     6601 tcaagccggg tcacacggtg gccatcgtcg gcccgaccgg tgccggcaag acgaccctgg
     6661 tcaacctcat catgcggttc tacgagctcg acggtggtcg aatcacgttg gacggcgtgg
     6721 atattcgtga tctgtcccgc caggggctgc gcgagcggat cgggatggtg ctgcaggaca
     6781 cctggctgtt cgccgggacg atccgcgaca acatcgccta cggtcggccg ggcgcgaccg
     6841 aggaagaggt gctcgcggcc tcgcgggcga ccttcgtcga tcggttcgtc cactcgctgc
     6901 cggacggtta cgacacggtg ctggacgcgg aggcatccaa cctctccgcc ggcgagcggc
     6961 agttggtgac gatcgcgcgc gcgttcctct ccgagccgtc gctgctcatc ctcgacgagg
     7021 ccacctcgtc ggtcgacacg cgcaccgagt tgctcgtgca gaaggcgatg cgggcgttgc
     7081 gttcggaccg gacgtcgttc gtcatcgccc accgcctgtc gacgatccgc gacgccgacg
     7141 tcatcctcgt catggaggca gggcgcatcg tcgagcaggg gacgcacgag gagctcctcg
     7201 cccagcacgg ggcctacgcg aggctgtatg ccgcgcagtt ctccggtgct gctgccgaag
     7261 ccgcggacgc cgccgccgag gccgccgtcg gagcctgacg acggagtccg gatgtcggag
//
LOCUS       DAOMZY010000162         7288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_9599_length_7288_cov_6.114890, whole genome shotgun sequence.
ACCESSION   DAOMZY010000162 DAOMZY010000000
VERSION     DAOMZY010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7288
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9599_length_7288_cov_6.114890"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PLK64_09965"
     CDS             <1..509
                     /locus_tag="PLK64_09965"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HPV80115.1"
                     /translation="REDLDAMPDDGHRHELLDGAIVMTPSPGYRHQRMSLRLAVLLLG
                     ACPEGLEVLTAPFDVVLSPRTVVVPDLIVARSGDFTEEDLPRAPLLAVEILSPSTRRL
                     DLTLKRDLYAEAGTAAYWVLDPQVPSITTWTLRGGTFEQTGYAAAADSLTADLPFPVT
                     VCPGDLVR"
     gene            complement(522..1718)
                     /locus_tag="PLK64_09970"
     CDS             complement(522..1718)
                     /locus_tag="PLK64_09970"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HPV80116.1"
                     /translation="MSVGDEVRRWRPDTYAVRGGLTRSEFDETSEAVFLTSGFVYDSA
                     QQAEAAFKDEIDKFIYSRYGNPTVAMFEERLRLIEGAEACFGTSSGMSAVFVALAALC
                     GAGDRIVASRALFGSCFVIIDEILPRWGVERVLVDGADLDQWRAALDRPTAAVFFETP
                     SNPMQELVDIRAVSDLAHAVGAKVVVDNVFGTPVYSKPLQHGADIVVYSATKHIDGQG
                     RALGGAILGPADYIGGPVKNLMRHTGPALSPFNAWVMLKGLETMSLRVNRQHDNALAV
                     AAFLAEHPRVTRVLHPWREDHPQHDLARRQMLGGGTVITFAIDGGKDDAFAVMNALQV
                     VDISNNLGDAKSLVTHPATTTHRRLTPEARAAVGITDGTIRLSVGLEDPADLIDDLAA
                     ALDALG"
     gene            complement(1715..2128)
                     /locus_tag="PLK64_09975"
     CDS             complement(1715..2128)
                     /locus_tag="PLK64_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPV80117.1"
                     /translation="MTYAGDISPTDTFAALQADPQAVLVDVRTSAEWAYVGVPDLARV
                     GRDAILIEWVTYPGGARNDNFVDQLREAGIAEGQSVYFLCRSGVRSKHAADAATEAGL
                     GPAYNIVDGFEGQLDATHHRGVGGWKTAGLPWRQG"
     gene            complement(2223..2813)
                     /locus_tag="PLK64_09980"
     CDS             complement(2223..2813)
                     /locus_tag="PLK64_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HPV80118.1"
                     /translation="MGSESTEPSGPPTAYLAGKLLVATPSVEGDVFRRSVVLMLHHDA
                     DGAQGVVLNQPLEAGVDAVLPGWQAVTTAPQTLFRGGPVGTDSAIGIVSVPGEGEATL
                     GVQRLFGGIGVVDLDAPPLLVAPEVAGLRIFAGYAGWSEGQLEGEITRGDWYVLDAEP
                     RDAFTDDPETLWASVLRRQRDSLALVAFYPDDPSMN"
     gene            complement(2818..5265)
                     /locus_tag="PLK64_09985"
     CDS             complement(2818..5265)
                     /locus_tag="PLK64_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPV80119.1"
                     /translation="MPTPKRSGAPKKARWTTAQKTASKSGPKKPHRGQAPTESKPPRA
                     ERAPRHVARTGSGDAPTSRYAARSRDERPARFERDERPSRPARFVRDDRPERPTRFDR
                     TDRPARFERDDRPARFERDDRSARPARVDRDGNPTRPARFERDDRPRRFDRDDRPARF
                     ERDDRPRRFDRDDRPARFERDDRPRRFDRDDRPARPARDDRPARFERDDRPSRPARDD
                     RPARDDRAARFERDDRPRRFDRDDRPARDSRPARDDRPARDDRAPRFERDNRLARPGR
                     FDRDDRPARPGRFDRDGGPSRPTRFERDDRPRRFDRDDRPARRDGERAAYREPFVDPE
                     SERLEADSWTSSARTPVSTNPVTVAEDNGFASLGLPERLVERLARDGITTPFPIQDAT
                     IPDALAGRDVLGRGQTGSGKTLAFGLPMLARLLADGSGRVPRRPRALVLVPTRELAMQ
                     VSDALEPLVHVVGMRHKLVAGGLSYTTQISALERGIDLLIATPGRLIDLMDRGAVELG
                     SVQVAVLDEADHMAEMGFLPEVTRILDEVPDGGQRLLFSATLDNGVDQLVSRYLTDAV
                     THSTDDATASVTTMRHFPFLIDPQHKKPLTAELANREGRTIVFVRTKLGADRVAAQLR
                     ECGVVAAALHGGLSQGVRNRVLGAFRLGTLRVLVATDVAARGIHVDDVSLVLQVDPPA
                     DHKDYLHRSGRTARAGEEGSVVTLVLPHQRKQTERLLREAGLEEVPTRVSPGDSLVAA
                     AGGNAPTGEPVTDEAMRRLLEPERRSPAGARGPRHGSHGGFRPHHGGAPRREDRRFDT
                     RDDRRPTSRDARRSVRD"
     gene            5445..6113
                     /locus_tag="PLK64_09990"
     CDS             5445..6113
                     /locus_tag="PLK64_09990"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HPV80120.1"
                     /translation="MSMPSEHRGPRPARVEVTGAGTASATPDVVRVALGIRCDSEGVA
                     AALSAAADTVRQVSAAARAHGLADRDIASTSASVQPRWNNDGSAVAGYTAYHQLTLLV
                     RSLPSLNDLVDAVADAAGNALVIDGITLDLAERGPLAEHAREAAFDDAQDKAQQYAAL
                     AGARLGRVLAVVEGGGVPGFEPMAGRRDAMMSMKASAGGMPVEAGEHSVGATVRVAWE
                     LVPE"
     gene            6182..6616
                     /locus_tag="PLK64_09995"
     CDS             6182..6616
                     /locus_tag="PLK64_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009376803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPV80121.1"
                     /translation="MATTELTADTFERTILDNDIVFVDFWASWCGPCRQFGPVFEKTS
                     AEHPDAVFGKVDTEAEQALAGALEITSIPTIMAFREGILVFSQPGALSAPVFEDLVGQ
                     VKALDMTDVRRQIAEHEAGHETGHEAGHDGAAEPQGAGTSQA"
     gene            6671..7099
                     /locus_tag="PLK64_10000"
     CDS             6671..7099
                     /locus_tag="PLK64_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HPV80122.1"
                     /translation="MRTILNIIWVVFAGFWLWLTYLAAGVLACLLIVTIPWGIASFRM
                     ANYALWPFGRTIVDKPDAGPASTIGNIVWIVCFGWWLALGHLTTAIAQAVTIIGIPLA
                     VANVKLIPVSLVPMGKDIVRIDDLGKATPWGPGPQRSFSV"
BASE COUNT         1091 a         2552 c         2585 g         1060 t
ORIGIN      
        1 cccgtgagga tctcgacgcc atgcccgacg acgggcaccg ccacgagctt ctcgacgggg
       61 cgatcgtcat gaccccgtcg cccggctatc ggcatcagcg gatgtcgttg cgtctggccg
      121 tcctgctgtt gggtgcctgc cccgagggtt tggaagtcct gacggcgccc ttcgacgtgg
      181 tcctctcgcc gcgcaccgtc gttgtccccg acctcatcgt ggctcgcagc ggcgacttca
      241 ccgaggagga cctgccgcga gctccgctgc tcgccgtcga gatcctctca ccgagcaccc
      301 gccgcctgga cctgacgctc aagcgggacc tctacgccga ggcggggacc gccgcctact
      361 gggtgctcga tccccaagtc ccgtccatca cgacgtggac actgcgcggg ggcaccttcg
      421 agcagacggg gtatgccgcg gccgccgact cgctcacggc cgacctgccg ttcccggtca
      481 ccgtgtgtcc gggagacctc gtgcgctgag cccgagggcg ctcagccgag ggcgtccagg
      541 gccgcagcca ggtcgtcgat gaggtcggcg gggtcttcca gacccaccga gagccggatc
      601 gtcccatcgg tgatccccac ggcggcccgc gcctccggag tcagccgccg gtgcgtcgtc
      661 gtcgccggat gagtgacgag cgacttggcg tcgccgaggt tgttcgagat gtcgacgacc
      721 tgcagggcat tcatgaccgc gaaggcatcg tccttgcccc cgtcgatggc gaaggtgatg
      781 actgtcccgc caccgagcat ctgtcgacgc gccaggtcgt gctgcgggtg gtcctcgcgc
      841 caggggtgca ggacccgcgt gacccgcggg tgctcggcaa ggaaagccgc caccgcgagc
      901 gcattgtcgt gctgtcggtt gacccgcagc gacatggtct ccagaccctt gagcatcacc
      961 caggcgttga agggcgagag cgccggaccg gtgtggcgca tgaggttctt caccgggcca
     1021 ccgatgtagt cggccgggcc gaggatcgct ccgccgagcg cccgaccttg accgtcgatg
     1081 tgcttggtcg ccgagtaaac gacgatgtcg gcgccgtgct gcaaaggctt ggagtagacc
     1141 ggcgtgccga agacgttgtc gacgacgacc ttggccccca ctgcatgcgc gaggtcggac
     1201 accgcacgga tgtcgacgag ttcctgcatg gggttgctgg gtgtctcgaa aaacactgca
     1261 gccgtaggtc ggtcgagggc tgcccgccac tggtcgaggt cggccccgtc gacgagcacc
     1321 cgctcgacgc cccaccgcgg caggatctcg tcgatgatga cgaagcagga gccgaacaac
     1381 gcgcgcgagg cgacgatccg gtcacccgca ccgcagaggg cggccagggc gacgaagacg
     1441 gccgacatcc ccgacgaggt cccgaaacac gcctcggcac cctcgatgag ccgcaaccgc
     1501 tcctcgaaca tcgcgaccgt cgggttgccg taccggctgt agatgaactt gtcgatctca
     1561 tccttgaacg ccgcctcggc ctgctgcgcc gagtcgtaga cgaaccctga cgtcaggaag
     1621 accgcttcgg aggtctcgtc gaactcgcta cgggtgaggc caccgcgcac ggcatacgtg
     1681 tcaggtcgcc accggcgcac ctcgtcgccg acgctcaccc ctgccgccac ggcaggccag
     1741 cggtcttcca cccgccgaca ccccggtggt gggtcgcgtc cagttgcccc tcgaacccgt
     1801 ccacgatgtt gtatgccggt ccgagaccgg cctcggtcgc cgcgtccgcg gcgtgcttgg
     1861 agcgcacccc cgagcggcag aggaagtaga cggactggcc ttcggcgatc ccggcctcgc
     1921 gcagttggtc gacgaagttg tcgttgcgcg cgccaccggg ataggtcacc cactcgatga
     1981 ggatggcgtc cctgccgacc cgcgcgaggt cgggcacgcc gacataggcc cactccgcgc
     2041 tggtgcggac gtcgacgagg acggcctgcg ggtcagcctg cagggcggcg aaggtgtcgg
     2101 tcggagagat gtcaccggcg taggtcatgg cagacagggt aacgatcacg gccgctggtc
     2161 agccccgcgc ttccacgctt cgacctgcgg cgtttccatc cgatccgcga ggagcagcgc
     2221 agtcagttca tcgacgggtc gtcgggatag aacgccacga gggccaggct gtcgcgttgg
     2281 cgtcgtaaca cgctggccca caaggtttcc gggtcgtcgg tgaaggcgtc ccgcggctcg
     2341 gcgtccagca cgtaccagtc cccccgcgtg atctcgccct caagttgccc ttcggaccac
     2401 ccggcatacc cggcgaagat ccgcaacccg gcaacctccg gggccacgag gagtgggggc
     2461 gcgtcgaggt cgacgacccc gatgcctccg aagaggcgtt ggacccccag cgtcgcctca
     2521 ccctcgcccg gaacgctgac gatgccgatc gccgagtcgg tgcccactgg accgccccgg
     2581 aagagcgtct gcggcgccgt cgtgacggcc tgccacccgg gcagcacggc atcgacgccc
     2641 gcttcgagcg gctggttgag aacgactccc tgggcgccgt cggcgtcgtg gtggagcatg
     2701 aggacgaccg atcggcgaaa gacgtcaccc tcgaccgacg gggttgcgac gagcaacttc
     2761 cccgcgaggt atgccgtcgg cggacccgac ggctcggtcg actccgagcc cacgccccta
     2821 gtctcgcacc gaccgacgcg cgtcacggga tgtcggccga cgatcgtcac gagtgtcgaa
     2881 ccgacggtcc tcgcgccgag gtgcgccacc gtggtgcgga cggaagccgc catggctgcc
     2941 gtgccgcgga ccgcgcgcgc ccgccgggct gcgccgctcg ggctcgagca gccggcgcat
     3001 ggcctcatcg gtgaccggct ccccggtcgg tgcgttgcca ccggcggccg cgacaagcga
     3061 gtcgccgggg ctgacccgcg tggggacctc ctcgagaccg gcttcgcgca acagccgctc
     3121 agtctgcttg cgctggtggg gcaggacgag cgtgacgacg ctgccctcct cccccgcgcg
     3181 agccgtgcgc ccggagcggt gcaggtagtc cttgtggtcg gccggcgggt cgacctgcag
     3241 gacgagagac acgtcgtcga cgtggatgcc gcgagcggcg acgtcggtgg cgacgagcac
     3301 ccgcaaggtc cccaggcgga acgcgccgag gacgcggttg cggacaccct gcgacagccc
     3361 gccgtgcaga gccgccgcga caacgccgca ctcgcgcagc tgagcagcga cccggtcggc
     3421 gccgagtttg gtgcgcacga acacgatcgt gcgaccctca cggttggcca actcagcggt
     3481 gagcggcttc ttgtgctgcg ggtcaatgag gaacgggaag tggcgcatcg tcgtcaccga
     3541 ggcagtggca tcgtcggtcg agtgggtcac ggcgtcagtg aggtagcgcg aaacgagttg
     3601 gtcgaccccg ttgtcgaggg tcgccgagaa cagcagccgc tggccaccgt cgggaacctc
     3661 gtcgaggatc cgggtcacct caggcaggaa gcccatctcg gccatgtggt cggcctcgtc
     3721 gaggacggcg acctggaccg agccgagctc gacggcgccg cggtccatga ggtcgatgag
     3781 gcggccgggg gtggcgatga gcaggtcgat gccacgctcg agcgcgctga tctgggtcgt
     3841 gtaggacagt ccgccggcca ccaacttgtg gcgcataccg acaacgtgca cgagcggctc
     3901 aagggcgtcg gacacctgca tcgcgagctc acgggtcggc acgagcacca gcgcgcgcgg
     3961 ccgacgcggg acccgaccgg acccgtcggc gagcagccga gcgagcatgg gcaggccgaa
     4021 cgccagggtc tttccggagc cggtctggcc acggccaagg acgtcgcggc cggcgagcgc
     4081 gtcggggatg gtcgcgtcct ggatcgggaa cggcgtggtg atgccgtcgc gggcgagcct
     4141 ctcgacgagc cgctcgggca ggccgaggga ggcgaaaccg ttgtcctcgg ccaccgtcac
     4201 cgggttggtc gagaccggcg tgcgggccga cgaggtccag gagtcggctt cgaggcgctc
     4261 gctctccggg tcgacgaacg gctcacggta tgccgcccgc tcaccatcgc ggcgcgcggg
     4321 tcggtcgtca cggtcgaagc ggcgcggccg gtcatcccgc tcgaaccggg tcggacgcga
     4381 gggtccaccg tcgcggtcga agcgacccgg acgggccgga cggtcatcgc ggtcgaagcg
     4441 acccggacga gccaggcggt tgtcccgctc gaaccgagga gcccggtcgt cgcgggcggg
     4501 tcggtcatca cgggccggac ggctgtcacg ggccgggcgg tcgtcccggt cgaagcggcg
     4561 cggccggtca tcccgctcga accgagccgc acggtcatca cgagcgggac ggtcatcacg
     4621 ggccggacgc gaggggcggt cgtccctctc gaaccgagcg ggacggtcat cacgcgccgg
     4681 acgggccggg cggtcgtcgc ggtcgaaacg ccgtggccgg tcgtcccgct cgaagcgcgc
     4741 cgggcggtcg tcgcggtcga aacggcgcgg gcggtcatca cgctcgaagc gcgccgggcg
     4801 gtcgtcgcgg tcgaaacggc gcgggcggtc atcacgctcg aagcgcgccg gacgtgtggg
     4861 gttgccatcc cggtcgaccc gggccgggcg tgccgagcgg tcatcccgct cgaaccgggc
     4921 cggacggtca tcgcgctcaa accgagccgg gcgatcggtg cggtcgaagc gggtcggacg
     4981 ctcggggcga tcgtcgcgga cgaaccgggc cggacggctc gggcgctcgt cgcgctcgaa
     5041 acgtgcgggg cgctcgtcgc gggagcgggc ggcataccgg gaggtgggag cgtcgcccga
     5101 accggtgcgg gcgacgtgcc tcggggcgcg ctcggcgcgc ggcggcttgc tctcggtggg
     5161 ggcctgtccg cggtgcggct tcttggggcc ggacttggag gcagtcttct gggcggtggt
     5221 ccagcgggcc ttcttgggcg cgccagaacg tttaggggtg ggcatgacgt ggatcagcta
     5281 cttccagtga ctcgagtaga cgcgtctcga gccaccgcca aggtgaccgg ataagacgtg
     5341 cctcaggcgc gcggtgcgcc cggaaatcgc cgggtcatgc ccggcgaacc tacctagtct
     5401 accagcgccg ccgcggccgc ccggctgccg tcgtaaggtg agccatgagt atgccgtccg
     5461 aacaccgtgg tccccgaccc gctcgcgtcg aagtgaccgg cgccggcacc gccagtgcca
     5521 cccccgatgt ggtccgcgtc gccctgggca tccgctgcga ctccgaaggc gtggccgctg
     5581 cgctctcggc cgcggctgac accgtccgtc aggtcagcgc ggccgcccgg gcacatggcc
     5641 tggccgaccg cgacatcgcc tcgaccagcg cctccgtcca gccgcggtgg aacaacgacg
     5701 gcagcgcggt ggcgggctac acggcatacc accagctcac cctgctcgtg cggtcgctgc
     5761 cgtccctcaa cgatctcgtc gacgccgttg ccgacgccgc gggcaacgcc ctggtcatcg
     5821 acgggatcac cctcgacctc gccgagcgcg ggccgcttgc agagcatgcg cgcgaggccg
     5881 ccttcgatga cgcgcaggac aaagcgcagc agtatgccgc cctcgccggc gcccgcctcg
     5941 ggcgggtcct cgccgtggtc gagggaggcg gcgtccccgg tttcgagccg atggcggggc
     6001 gacgcgacgc gatgatgtcg atgaaggcct ccgcgggagg gatgccggtg gaggccggcg
     6061 agcactcggt gggcgccacc gttcgggtcg cctgggaact cgtccccgaa tgaccgaagc
     6121 cgtctgtggc tggacgggtt gagtctggaa tagcttccgg gcgggcgcag ttggcacagc
     6181 catggcaaca accgaactca ccgccgacac cttcgagcgg accatcctgg acaacgacat
     6241 cgtcttcgtc gacttctggg cctcctggtg tggcccgtgc cgtcagttcg ggccggtctt
     6301 cgagaagacc tccgcggagc accccgacgc cgtcttcggc aaggtcgaca ccgaggccga
     6361 gcaggccttg gccggagccc tggagatcac gtcgatcccg acgatcatgg ccttccgcga
     6421 gggcatcctc gtgttctcgc agcccggcgc cctctcggcc ccggtcttcg aggacctcgt
     6481 cggccaggtc aaggccttgg acatgaccga cgtgcgtcgc cagatcgccg agcacgaggc
     6541 cgggcatgag actgggcacg aggctgggca cgacggcgcg gccgagccgc agggtgccgg
     6601 aacctcccag gcctgagcaa cgttcacggc ataccagagc aacacctcgc accaccggaa
     6661 agaagcgccc gtgcgcacca tcctcaacat catctgggtc gtcttcgccg gcttctggct
     6721 ctggctgacc tacctcgccg ccggcgttct ggcctgtctg ctgatcgtca ccatcccgtg
     6781 ggggatcgcg tcgttccgga tggccaacta cgccctgtgg ccgttcgggc gcaccatcgt
     6841 cgacaagccc gacgccgggc cggcttcgac gatcggcaac atcgtctgga tcgtctgctt
     6901 cggctggtgg ctggccctcg gacacctcac gaccgccatc gcgcaggccg tgaccatcat
     6961 cggcatcccg ctggcggtgg ccaacgtcaa gctcatcccg gtgtcgctgg tgccgatggg
     7021 caaggacatc gtccggatcg acgacctcgg caaggcgacc ccgtggggtc ccggcccgca
     7081 gcgctccttc agcgtctgac gcgcaccagc tgacctgccc cagcactcgc cgcaacacga
     7141 aaggcgccca cccggaaagc ccggggggcg cctttcgcgc tgtccagcgt gacgtgtcca
     7201 cacggcatac gggctgctga ccgatgccga gtagtggagg tggcgggaat cgaacccgcg
     7261 tccgatgacg tgatcccagg acttctcc
//
LOCUS       DAOMZY010000163         7273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_9624_length_7273_cov_6.399418, whole genome shotgun sequence.
ACCESSION   DAOMZY010000163 DAOMZY010000000
VERSION     DAOMZY010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7273
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9624_length_7273_cov_6.399418"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="PLK64_10005"
     CDS             complement(<1..1120)
                     /locus_tag="PLK64_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HPV80123.1"
                     /translation="MRILMIGAGGVGDAAARIAVERDFFEALVVADYDLGRARATVAA
                     ASARRPGESRLVAAYVDASDAESVARLAREHQITHVFNAVDPRFVMSIFAGARAAGAN
                     YLDMAMSLSRPHETRPHELTGVKLGDEQFAQAGEWEAEGRLALVGIGVEPGLSDVFAR
                     YAADHLFTTIDELGTRDGANLVIRDETGAEVFAPGFSMWTIIEECLNPPVVWEKDRGW
                     FTLAPFSEPEIFDFPEGIGPVECVHVEHEEVLLMPRWVDAKKVTFKYGLGEQMITILR
                     TLHALGLDRTDPIRVKGVEVSPRDVVAACLPDPATIGPRMTGKTCAGVWVTGTGRDGA
                     PRATYLYHVVDNEWSMREYGAQCVVWQTAVNPIIALELL"
     gene            complement(1134..1913)
                     /locus_tag="PLK64_10010"
     CDS             complement(1134..1913)
                     /locus_tag="PLK64_10010"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV80124.1"
                     /translation="MIVALLVLFYLFLPVLYTFVFSFNNYKKSNISWNPEGSPTLEHW
                     QNPCGPPGLCESLGTSIKVGGIATLVATVLGTMLAFALVRHRFKGRGAGNILVFIPMA
                     TPEIVLGASLLTLFVQGFGQYGLKLGFWTIVFAHIMFCISFVVVTVKARLQSLDPRLE
                     EAAQDLYAGPGSTFWRVTFPLVLPGIVSAALLSFSLSFDDFIITNFVSGDTSTFPKFV
                     YVSYLRGIPAQANVIGFSMFVIAVLLVVVGQVIGSRRRSPA"
     gene            complement(1958..2875)
                     /locus_tag="PLK64_10015"
     CDS             complement(1958..2875)
                     /locus_tag="PLK64_10015"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV80125.1"
                     /translation="MSALAHIGGSPSGTSTTAAPEPTKRGGMAAYLLLLPGLIWLLVF
                     FIAPLVQLATVSLQSQFPGYPGYYYRNVDFGNYGRAIVEYWPHFVRSLLFAGLATFFA
                     FVLAYPLAYAMAFKAGKWRGVMMICVIAPFFTSFILRTIAWRQILSDEGPVVSVLNFL
                     HVLPGGHITETWVAVVAGLTYNFLPFMVLPIYASLERADPKIIEAGGDLYANGFTTFR
                     TVTLPMSMPGVLAGTLLTFIPAAGDYVNAELLGSDISTKMVGNGIESQFFKVLGGYPI
                     AAALSFTLMAVILALVFLYVRRYGTEELL"
     gene            complement(2872..3972)
                     /locus_tag="PLK64_10020"
     CDS             complement(2872..3972)
                     /locus_tag="PLK64_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV80126.1"
                     /translation="MAEFRAAKGDLVLENVTKSFTNFTAVDDLSMTVPRGAFFALLGP
                     SGCGKTTTLRMVAGLEQPTSGRVLIGDTDLTGSRPYERPVNTVFQSYALFPHLTVRDN
                     VAFGPKRRGAKDAQAQADATLALVQLTHLAGRKPTQLSGGQQQRVAVARALVNHPEVL
                     LLDEPLGALDLKLRREMQVELKRIQTEVGLTFVHVTHDQEEAMTMADTVAVMNLGKIE
                     QMGAPEVLYDLPRTKFVANFLGQSNLLSGHVDDTDGEHIIVSAAHHKLRLRKSRAAAG
                     SGDIAFGVRPEKTRIHNTDPGASAAGNSIGPGTLLDVSFTGVATQYLVDVPDAGRWAV
                     YEQNLDLDRIDSRPGDTVWISWDPGHAFGVRP"
     gene            complement(3972..5213)
                     /locus_tag="PLK64_10025"
     CDS             complement(3972..5213)
                     /locus_tag="PLK64_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HPV80127.1"
                     /translation="MRRSEPPRDPQARALASLAASSLSRRRLLSGAGLGLAGAGLAAC
                     APPLPPAATGPTGPKLPVDKSATDKTVAFANWTAYLDYDEEAKNYPTLEAFIKETGIQ
                     ASYTEDIEDNDAYVSKIKPQLQATPPQDINRDIFALTDWMANRVIRDGLVQPLDLIRM
                     PHAANLLDSLKDVSFDPGRKYSLTWQSGFAGIGYDKSKVGRELKSLSDLWADDLKGKV
                     VALSEMRDTLGLIMQSQGVDISGSFTKDQVSKAMDEVSKRIADQNIRRIKGNSYMEDL
                     KSGNAIAGIVWSGDLFILRAETENENWEFVIPDSGGTLWSDNMMIPITSTHRRNAMAL
                     MDYYYRPDVAAQVAAYVNYVCPVVGAQAELAKTDPELAESPFIFPTSEWIAEHKIQGF
                     RALDAQEDADYSAMWAKVVGN"
     gene            complement(5343..6791)
                     /locus_tag="PLK64_10030"
     CDS             complement(5343..6791)
                     /locus_tag="PLK64_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HPV80128.1"
                     /translation="MSQAPRVLRNHVGGEFVDATADARSDVVNPATGQVVATAPVSGQ
                     EDVNRAYAAASAAFEEWGRTTPRERQEALLKFAAAVEARADDFVRLEGENTGKPHALT
                     ASEEVPPMLDQIRFFAGAARVLEGRSAGEYMPGHTSWIRREPIGVIGQVTPWNYPMMM
                     AIWKIAPALAAGNTVVLKPSDTTPETTLLLAALAADCFPAGTFNVVTGDRETGRMVVS
                     NPRADMVAITGSVRAGSQVAAAAAESVKRVHLELGGKAPVVVFDDADIEAAVEGIAIA
                     GYFNAGQDCTAATRVLAGPGIYDDFVAALGEYAANSAKVGMPDDEDALFGPVNNPAQL
                     ERVTGFLNRVPAHASVVAGGSRVTDLGDGYFIAPTVLAGLRQDDEHIQKEIFGPVITV
                     QRFSDEDEAIRWANGVDYGLASSVWTKDFGRAMRMSRALDFGCVWINTHIPIVAEMPH
                     GGYKHSGYGKDLSMYGLEDYTRIKHVMANIQA"
     gene            7013..>7273
                     /locus_tag="PLK64_10035"
     CDS             7013..>7273
                     /locus_tag="PLK64_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HPV80129.1"
                     /translation="MRRSTRRPTRQVLDATAKAIIEQLQEDGRRSYAAIATAVGLSEA
                     AVRQRVQKLVDGGVVQIVAVTDPLHVGFLRQAMVGIKVQGDIG"
BASE COUNT         1249 a         2342 c         2506 g         1176 t
ORIGIN      
        1 cgagcagttc cagcgcgatg atggggttga ctgcggtctg ccacacgacg cattgggctc
       61 cgtactcacg catcgaccac tcgttgtcga ccacgtggta gaggtaggtg gcccgtggcg
      121 caccgtctct tccggtgccc gtcacccaga ccccagcgca ggtcttgccg gtcatccgcg
      181 gcccgatcgt ggccgggtcg ggcaagcagg cggccacgac gtcgcgcggc gacacctcga
      241 cccccttgac ccggatcgga tcggtgcggt cgaggccgag ggcgtgcaag gtgcgcagga
      301 tggtgatcat ctgctcaccc aggccgtact tgaaggtgac cttcttggca tcgacccagc
      361 gaggcatgag cagcacttct tcatgctcga cgtgaacaca ctcgaccggc ccgatgcctt
      421 cggggaagtc gaagatctcc ggctcactga acggcgcgag ggtgaaccag ccacggtcct
      481 tctcccagac caccggcggg ttgaggcact cctcgatgat cgtccacatc gagaagcccg
      541 gcgcgaagac ctccgcgccg gtctcgtccc ggatgacgag gttggcgcca tcgcgcgtgc
      601 cgagttcgtc gatcgtcgtg aacaggtggt cggcggcata cctcgcgaac acgtccgaca
      661 gccccggttc gacgccgatc cccaccaacg cgagccggcc ctcggcctcc cactcccccg
      721 cttgcgcaaa ctgctcgtcg ccgagtttga cgccggtcaa ctcgtgcggg cgggtctcgt
      781 gcggtcgcga caacgacatc gccatgtcaa ggtagttcgc tcctgccgcg cgcgcgccgg
      841 cgaagatcga catgacgaag cgcgggtcca cggcgttgaa gacgtgggtg atctggtgct
      901 cgcgcgccag cctcgccacc gactcggcat cggaggcatc gacgtatgcc gccaccaacc
      961 ggctctcgcc gggccttcgg gcactcgccg ccgcgaccgt ggcccgggct cggcccaggt
     1021 catagtccgc gacgacgagc gcctcgaaga agtcccgttc gacagcgatc cgggcggcgg
     1081 cgtctccgac cccgccggcc ccgatcatga ggatccgcac gagctatcta ctcctaggcc
     1141 ggcgagcgcc gacgagagcc gatgacctga cccacgacga ccagcaggac ggcgatgacg
     1201 aacatcgaga aaccgatgac gttggcctgg gccgggatgc cacgcaggta ggagacgtac
     1261 acgaacttcg ggaaggtcga cgtgtcgccc gagacgaagt tggtgatgat gaagtcgtcg
     1321 aacgacaacg agaacgacaa caacgccgcc gacacgatgc ccggaagcac gagcgggaag
     1381 gtgacccgcc agaaggtgct gcccggaccg gcatacagat cctgcgcagc ctcctccaag
     1441 cgagggtcga ggctctgcag ccgcgccttg acggtgacga cgacgaagct gatgcagaac
     1501 atgatgtggg cgaagacgat cgtccagaac ccgagcttga ggccgtactg gccgaacccc
     1561 tggacgaaga gggtgagcag tgaagcaccc aggacgatct ccggcgtcgc catcgggatg
     1621 aagacgagga tgttcccggc tccacgcccc ttgaagcgat ggcgcacgag cgcgaaggcg
     1681 agcatcgtgc cgagcacggt ggccacgagg gtggcgatcc cacccacctt gatcgacgtg
     1741 cccaacgact cgcacagtcc gggaggacca cacgggttct gccagtgctc gagggtcggt
     1801 gacccctcgg ggttccagga gatgttcgac ttcttgtagt tgttgaacga gaagacgaac
     1861 gtgtaaagca ccggaaggaa gagatagaag aggacgagca acgcgacgat catcgcgaag
     1921 cgttcggcga accagaactt cagccgctga cctgtgctca cagcagttcc tccgtcccgt
     1981 aacggcgcac gtagaggaac acgagcgcga ggatgacggc catcagggtg aaggacaggg
     2041 ccgccgcgat cggatagcca ccgagcacct tgaagaactg gctctcgatg ccgttgccga
     2101 ccatctttgt ggagatgtct gatcccagga gttcggcgtt gacgtagtca ccggccgccg
     2161 ggatgaaggt gagcagagtg ccagcgagca cacccggcat cgacatcggc agggtcacgg
     2221 tccggaacgt cgtgaagccg ttggcataga ggtcgccacc ggcctcgatg atcttgggat
     2281 cggcgcgctc gagcgaggcg tagatcggca ggaccatgaa cggcaggaag ttgtaggtca
     2341 gacccgcgac caccgccacc caggtctcgg tgatgtgccc gcccggcagg acatggagga
     2401 agttgagcac ggacacgacc gggccctcgt cggagaggat ctggcgccag gcgatggtgc
     2461 gcaggatgaa ggacgtgaag aacggcgcga tgacgcagat catcatcact ccacgccact
     2521 tgcccgcctt gaatgccatg gcataggcca acgggtaggc caggacgaac gcgaagaacg
     2581 tcgcgaggcc ggcgaacagc agcgaccgca cgaagtgcgg ccaatactcg acaatggccc
     2641 ggccgtagtt cccgaagtcg acgttgcggt agtagtagcc cggatatccc gggaactgcg
     2701 actgcaacga cacggtcgcc agttggacga ggggggcgat gaagaagacc aacagccaga
     2761 tgaggcccgg caacagcagc aggtatgccg ccatcccccc tcgcttggtg ggttccggcg
     2821 ccgccgtggt cgacgtcccg ctggggctcc caccgatgtg cgcgagggcg ctcatggcct
     2881 gaccccgaag gcgtgcccgg ggtcccagct gatccacacc gtgtcgcccg ggcggctgtc
     2941 gatccggtcc aggtcgaggt tctgctcgta gaccgcccaa cgcccggcgt cggggacgtc
     3001 gacgaggtac tgcgtggcga cgccggtgaa ggacacgtcc agcagggtgc cggggccgat
     3061 gctgtttccg gctgcgctcg cgcccgggtc ggtgttgtgg atccgtgtct tctcggggcg
     3121 gaccccgaag gcgatgtcgc cgctgcccgc cgctgcccgg ctcttgcgca accgcaactt
     3181 gtggtgagcg gcagacacga tgatgtgctc gccatcggtg tcgtcgacgt ggcccgagag
     3241 caggttggac tgacccagga agttggcgac gaacttggtc cgcggcaagt cgtagaggac
     3301 ctcgggcgca cccatctgct cgatcttgcc gaggttcatc accgcgaccg tgtcggccat
     3361 ggtcatggcc tcctcctggt cgtgggtgac gtggacgaag gtgagaccga cctcggtctg
     3421 gatccgcttg agctcgacct gcatctcgcg acgcagcttg aggtcgaggg caccgagcgg
     3481 ctcgtcgagc agcagcacct cggggtggtt gaccagtgcc cgggcgacgg caactcgctg
     3541 ctgctgaccg cccgagagct gggtcggctt gcgcccggcg aggtgggtga gttggaccag
     3601 cgcgagagtc gcatcggcct gggcctgggc gtccttggct ccccgccgct tcggaccgaa
     3661 ggcgacgttg tcccggacgg tgaggtgcgg gaagagggcg taggactgga agacggtgtt
     3721 gactggccgc tcgtagggac gggaccccgt gaggtcggtg tccccgatga gcacccggcc
     3781 gctggtcggc tgttcgaggc cggcgaccat ccgcagcgtc gtcgtcttgc cacacccgga
     3841 cgggccgagc agagcgaaga acgccccccg gggcacggtc atcgacaggt catcgacggc
     3901 ggtgaagttg gtgaagctct tcgtgacgtt ctccaggacg aggtcgccct tggctgcgcg
     3961 gaactcggcc atcagttgcc caccaccttt gcccacatcg cggagtagtc cgcgtcctct
     4021 tgggcgtcca gcgcccggaa cccctggatc ttgtgttctg cgatccactc ggaagtgggg
     4081 aagatgaacg ggctctcggc gagctcgggg tcggtcttgg ccaactccgc ctgggcgccg
     4141 acgaccggac agacgtagtt gacgtaggcc gcgacctggg ctgccacgtc ggggcggtag
     4201 tagtagtcca tcagcgccat cgcgttgcgg cgatgggtcg aggtgatggg gatcatcatg
     4261 ttgtcgctcc agagcgtgcc gccggagtcg gggatgacga actcccagtt ctcgttctcg
     4321 gtctcggccc gcaggatgaa caggtcaccg ctccagacga tgccggcgat ggcgttgccg
     4381 ctcttgaggt cctccatgta ggagttgccc ttgatccggc ggatgttctg atcggcgatc
     4441 cgcttggaca cctcgtccat ggccttggag acctggtcct tggtgaacga gccggagatg
     4501 tcgacgcctt gcgactgcat gatcaggccg agggtgtcgc gcatctctga gagggccacg
     4561 accttgccct tgagatcgtc ggcccacagg tccgacaggc tcttgagctc gcggccaacc
     4621 ttgctcttgt cgtagccgat tccggcgaaa ccgctctgcc aggtcagtga gtacttgcgg
     4681 cccgggtcga aggacacgtc cttgagggag tcgagcaggt tggcggcgtg cggcatccgg
     4741 atgaggtcca gcggctgcac cagaccgtcg cggatcaccc ggttggccat ccagtccgtg
     4801 agggcgaaga tgtcccggtt gatgtcctgc ggcggggttg cctgcaactg cggcttgatc
     4861 ttggagacgt aggcgtcgtt gtcctcgatg tcctccgtgt aggaggcctg gatgccggtc
     4921 tccttgatga aggcctccaa cgtcgggtag ttcttggcct cctcgtcgta gtcgaggtag
     4981 gccgtccagt tggcgaaggc gacggtcttg tcggtggccg acttgtcgac gggcagcttc
     5041 gggccggtcg ggccggtcgc ggccggcggc aacggcggtg cacacgcggc caggcccgca
     5101 ccggccaggc cgaggccggc gccggacagc agccgacgac gggacagaga gcttgccgcg
     5161 agcgaggcca gggcccgcgc ctgcggatca cgaggtggtt cggatcggcg catcgcaccg
     5221 ctccttcctt gagaggaatg ccgggccgtc gaggccgcgg tagggacgac gtgcggcgga
     5281 gccaggcccg ccggggagtg ggttgagaag gttccggccg agggatcggc gcggtgattg
     5341 cgtcaggcct ggatattggc catgacgtgc ttgatacggg tgtagtcctc caagccgtac
     5401 atcgacaggt ccttgccgta accgctgtgc ttgtatcccc cgtgtggcat ctcggccacg
     5461 atcggaatgt gcgtgttgat ccacacacac ccgaaatcca aggcccgcga catccgcatg
     5521 gcccgcccga agtccttcgt ccagaccgac gacgccaagc cgtagtcgac gccgttggcc
     5581 caccggatcg cctcgtcctc atcggagaag cgctgcacgg tgatgacggg cccgaagatc
     5641 tctttctgga tgtgttcgtc gtcctggcgc aggccggcga gcaccgtcgg ggcgatgaaa
     5701 tacccatcgc cgaggtcggt gacgcgggag ccgccggcca ccacagacgc gtgggccggc
     5761 acccggttga ggaagcccgt cacccgctcc aactgcgcgg ggttgttgac cggcccgaag
     5821 agcgcatcct cgtcgtcggg catgccgacc ttggccgagt tcgcggcata ctcccccagg
     5881 gcggcgacga agtcgtcgta aatcccggga ccggcgagca cccgggtggc cgccgtgcag
     5941 tcctgccccg cgttgaagta gcccgcgatg gcgatcccct cgacggccgc ctcgatgtcg
     6001 gcgtcatcga agacgacgac cggcgccttg ccgccgagct caaggtggac ccgcttgacg
     6061 ctctcggcgg cagcggcggc gacctgcgag ccggcccgca ccgacccggt gatggcgacc
     6121 atgtcggccc gggggttcga cacgaccatc cggccggtct cgcggtcccc ggtgacgacg
     6181 ttgaaggtgc cggcggggaa acagtcggcg gccaacgcag cgagcaacag ggtggtctcg
     6241 ggggtcgtgt cactcggctt gaggacgacc gtgttgcctg ccgccagggc aggggcgatc
     6301 ttccagatgg ccatcatcat cgggtagttc cagggggtga cctggccgat cacgccgatg
     6361 ggttcgcggc ggatccacga ggtgtgcccc ggcatgtact cccctgccga gcgtccctcc
     6421 agaacgcggg cggcgccggc gaagaagcgg atctggtcga gcatcggcgg cacctcctcg
     6481 ctcgcggtga gcgcgtgcgg cttgccggtg ttctcgccct ccaggcggac gaagtcgtcg
     6541 gcccgagcct ccaccgcagc ggcgaacttc agcagggcct cctgccgctc gcgcggcgtc
     6601 gtgcggcccc actcctcgaa ggccgcggaa gcggcggcat acgcccggtt gacgtcctct
     6661 tgcccggaga cgggggccgt ggcgacgacc tggccggtcg ccgggttgac gacatcgctg
     6721 cgcgcatcgg ccgtcgcgtc gacgaactcg ccgccgacgt ggttgcgcaa cacgcgtggg
     6781 gcttgagaca tagagacctc cgttgagctg gccgccggct cccggtccgg acgcggtttc
     6841 gggcaatcta gcccgctggc gaccgttttc ggtagtggca aggcatagaa actttgcatt
     6901 ctgcgctccc tggaccccaa aaacgataga ttccgaaggg tcgagcccgg ccgggccttg
     6961 ccaaccggca ccgcagcggg cacgatcaga ccccatgaca tcgggaccgt ccatgagacg
     7021 ttcgacccgc cgcccgaccc ggcaagttct cgacgccacc gcgaaggcga tcatcgagca
     7081 acttcaagag gacggacgtc ggtcgtatgc cgcgatcgcg accgcagtgg ggctgtccga
     7141 ggcggccgtc cgacaacggg tgcagaaact cgtcgacggc ggggtcgtgc agatcgtcgc
     7201 cgtcaccgac cccctgcatg tcggcttcct ccgtcaggcg atggtcggga tcaaggtcca
     7261 gggcgacatc ggc
//
LOCUS       DAOMZY010000164         7261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_9646_length_7261_cov_5.699417, whole genome shotgun sequence.
ACCESSION   DAOMZY010000164 DAOMZY010000000
VERSION     DAOMZY010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7261
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9646_length_7261_cov_5.699417"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1619)
                     /locus_tag="PLK64_10040"
     CDS             complement(<1..1619)
                     /locus_tag="PLK64_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPV80130.1"
                     /translation="MHRTEDVQVVVDLGNTHVRVMSVTPDGASPPATRVLDMDGRMPA
                     LVGCAADGTWSWGVDRARGRTVIGGFLARVDDPVPLVIGDVRVSAAHVVARQLGDAVD
                     LVAAEHGRPSLLTVIHPDDLSPHGRRQIEAALDTDLPVRWASRAAVSIHEALDRGEVA
                     VGDVVAALHVGGASCVATVWALTPTPRRLAVVSGDGSCAGGALDDLVVAHVRRSVDAH
                     EGVDPAALRASCEEAKSALSTQTSVDVGVGETRVRLVRTELEELSAPLVERRLDTLAE
                     ALRVAEVEPAALRAVLLSGGGASAPCVVEAIGARFATPARLMTRRASAIDHDPTVLAQ
                     PAPLPVATAAEGRTRRRFGARMAAAHLTARRVATPSSPHARQAPTPRMPVLVGSALVA
                     VLVVGGGAVQQAGLVGMLATALGTEGPGGVSARSLASGVVAPWLNVIPELPESVVVAI
                     EEATSTVTDGDSDNDGTTDAGDTATSTDVVGVNGPGGGAVQPGPSSTSPRTPGTTTTP
                     RPGSPAPTSSPNPTSDPTPDPDPTPEPTPDQ"
     gene            1834..2976
                     /locus_tag="PLK64_10045"
     CDS             1834..2976
                     /locus_tag="PLK64_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015938242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IniB N-terminal domain-containing protein"
                     /protein_id="HPV80131.1"
                     /translation="MAITASLLDFILNLLRDDAAAAEYNDNPDAALAAAGLGDITCAD
                     MDAVRPVVLDYADVSQGDHEREYETGGNSVSYGDAGGGHGGGHGGGGGHGGGHDDDDD
                     GHGHGGGGGHGDDDHGHAVAQLTSIVNNYTYSTTIDDRDTLTDMSVNQNIWADGDVTQ
                     LFDNDAIIASGDGAIAAGGSVAVGSFNEDSNNTDNSVEIEDSFNTDNSLELDVEIEDS
                     FNDNSDNSTSDDDGIDNDGGDNDGIDVDNSGNDNSDNSDNSDDDGIDNDGGDNDGIDV
                     DNSGNETDNSQENDNSVDNSQENDNSVDNSQENDNSVDNSSEDENSIEVEFEGSFNDD
                     NSDDDGFDVDNSGNDNSSDNSDDDGFDVDNSGNTDDNSDDDGVDLL"
     gene            3241..3990
                     /locus_tag="PLK64_10050"
     CDS             3241..3990
                     /locus_tag="PLK64_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80132.1"
                     /translation="MAFIRRVRTGSGATAVQIAEYANGRQRIVEHVGSAHTEAELGVL
                     LARACELLEDPAQGALDLAVEPSVPVASIVAAPADAALFDPSPVAAPGRAGPGKVVGT
                     HSRLLFDALAGVYAALGFDAVGDAVFRDLVIARVVEPTSLLDVARVLTDLGRAPASYP
                     TMRRTLARAHKRGYRDLVAKACFEHASTRGDISLVLYGVTTLYFEAEKEDDLRKVGYS
                     NYAEVVVMPRSSCWGGCSCWSGAREGEARAA"
     gene            complement(4007..5005)
                     /locus_tag="PLK64_10055"
     CDS             complement(4007..5005)
                     /locus_tag="PLK64_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPV80133.1"
                     /translation="MTAPTLPSLLQGFFTERLVRQLQASPQTITAYRDTIKLLLLFAA
                     QATKKAPARLTIDDLDAVMVGAFLDHLDTERGNSTATRNARLAAIHSLYRYALPLIPE
                     RAHTASQVLAIPQRRHDRAIVSYLTGPETDALLAAPDRTTWHGRRDHALLLTAVQTGL
                     RLSELTGLTIQDIITTSPGAAVHTTGKGRRERATPLTRQTTAVLLAWLPQAGPTPSSP
                     AFPTRSGTRMSADAVQRLVAKHAATAALECPSVASKHVTPHTLRHTAAMALMSAGVDT
                     SVIALWLGHQNATTTHIYLHADMTIKERALSRVPAANTTPGRYRPGDDLIDFLERL"
     gene            complement(5002..5955)
                     /locus_tag="PLK64_10060"
     CDS             complement(5002..5955)
                     /locus_tag="PLK64_10060"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPV80134.1"
                     /translation="MSTLREDVTDYLATRRAMGFKVEGLSKLLSNFVTFCEARGASHV
                     RNDLAVAWATTPIKVPVTDALIARRMDAVRVFARHQHALDPSTQIPAEGIGNRRYQPR
                     EPNVFTEAQVLALVVAADTLSPRFKAITWRTLIGLLAATGMRPGEACRLSVDDVDLAG
                     GVLQVLDTKFGKSRLVFIHPTTATVLADYLQVRRDWVGTTARACPAVFVNSRREAINP
                     DRLGATFRQVVAAAGVTAKPGHRPPRLHDLRHTYTVATIQGWYRDGHDVQARLPLLST
                     WLGHVDPASTYWYLHAVPELLAHAATRLEPQASPPTGERTP"
     gene            complement(5952..6950)
                     /locus_tag="PLK64_10065"
     CDS             complement(5952..6950)
                     /locus_tag="PLK64_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPV80135.1"
                     /translation="MDRVSSVDVRGGSASVSELLVEFAAWLDRQRGLAPVTVHNYCWN
                     ARQFLDALPESTQVSVGLLDAGTVTAFMVEFCRGRNTNSAKSMARSVRSFLRFAHATG
                     RTSTELWGAVPASSGWHLASLPKAVPGADLERLLTVAARLRFTEAGRRDYAILLVLAR
                     LGLRRGEVAGLRLDDIDWRSGELTVVGKGGRVERLPLPVEPGEAIVAWLTDGRPECDT
                     RSVFTTLRPAGQPLSAGAIGHVVGSACQRAGLARIGAHQLRHSLATAMLRAGASLPEV
                     GQVLRHRSARSTAIYAKVDEVALRPLARPWPGTAPGPALPDAAARSLALPWPGGRS"
BASE COUNT         1129 a         2442 c         2604 g         1086 t
ORIGIN      
        1 tcctgatccg gggtgggttc cggggtgggg tccgggtcgg gggtggggtc cgacgtgggg
       61 ttggggctcg acgtgggggc gggggagccg ggccgaggtg tcgtcgtcgt gccgggagtg
      121 cggggcgacg tcgagctggg gccgggttgg acggccccac cgccgggccc gttgaccccc
      181 acgacgtccg tggaggtggc ggtgtcgccg gcgtcggtcg tgccgtcgtt gtcggagtcg
      241 ccgtcggtca cggtactcgt cgcctcctcg atggccacga cgacgctctc gggcagttcg
      301 gggatgacgt tgagccacgg tgcgaccact ccggaggcca gggaccgagc cgacacgccg
      361 ccgggacctt cggtgccaag ggccgtcgcc agcatgccga ccaaccctgc ctgctggacc
      421 gcgccgcccc ccacgacgag caccgcgacc agagcggagc ccacgagcac cggcatcctg
      481 ggtgtcgggg cctggcgggc gtgcggcgat gacggtgtgg cgacccggcg ggcggtcagg
      541 tgtgccgctg ccatccgggc gccgaagcgg cggcgcgtcc ggccttcggc tgcggtggcc
      601 acgggtaggg gcgcgggctg cgcgagcacg gtggggtcgt ggtcgatggc tgacgcacga
      661 cgggtcatca gccgggccgg ggtcgcgaac ctcgcgccga tggcctcgac gacgcacggg
      721 gcactcgcgc cgccaccgct gagcagcacc gcgcgcaggg ccgcgggctc gacctcggcc
      781 acgcgaaggg cctctgccag cgtgtcgagc cggcgctcca cgaggggagc cgacagctcc
      841 tccagttccg tgcgcaccag gcgcacccgg gtctctccta ccccgacgtc gactgaggtc
      901 tgggtggaca gggccgactt cgcctcctcg cacgacgccc ggagggcggc cgggtcgacc
      961 ccctcgtggg cgtcgacact tcggcggacg tgggcgacga cgaggtcgtc gagggctcct
     1021 ccggcgcacg agccgtcgcc gctcacgaca gcgaggcgcc ggggcgtggg cgtcagggcc
     1081 cagactgtgg cgacacacga ggcccctccg acatggagcg cggccacgac gtcgcccacg
     1141 gcgacctcac cgcggtcgag agcctcatgg atgctcacag cagcccggct cgcccaccgc
     1201 accggaagat ccgtgtcgag agctgcctcg atctgccggc gcccgtgggg gctcaggtcg
     1261 tcggggtgga tcacggtgag gaggctggga cgcccgtgct ccgcggcgac gaggtcgacg
     1321 gcatccccga gctggcgagc cacgacgtgc gctgccgaca ctcgaacgtc cccgatgacc
     1381 aggggcaccg ggtcatcgac ccgggccagg aagccgccga tcaccgttcg tccccgcgcc
     1441 cggtccacac cccagctcca cgtcccgtcc gcggcgcagc cgaccagcgc cggcatccgt
     1501 ccgtccatgt cgagcacccg tgtcgcaggg ggcgatgcgc cgtccggggt cactgacatc
     1561 acccggacat gggtgttccc gaggtcaacg acgacctgga catcctctgt gcggtgcacg
     1621 tcaattctcc ctgagcattt ccccgcgttt ccgatatggc ctccggggcc acccctatcg
     1681 tggcccacga tgtggggggg gcgccacccc cctagcccct aatgggccga acggtggagg
     1741 gggggttccc ccgatgcggg tggaccgcag catccgtacg ttcgccgtgg gagaactccc
     1801 ggcaccacct cagaccaccc actggagggg atcatggcaa tcacagccag cctgctcgac
     1861 ttcattctca acctgctccg cgacgacgcc gccgccgccg agtacaacga caacccggat
     1921 gctgcccttg cggccgcggg cctcggagac atcacgtgcg ccgacatgga cgcggttcga
     1981 cccgttgtcc tcgactacgc cgacgtcagc cagggtgacc acgagcgcga gtacgagacg
     2041 ggtggcaaca gcgtctccta cggcgatgca ggcggcggcc acggcggcgg tcacggcggc
     2101 ggcggcggtc acggtggcgg ccacgacgac gacgacgatg gccacggcca cggcggcggc
     2161 ggcggccacg gggacgacga ccacggccac gccgtggcgc agctgacctc gatcgtcaac
     2221 aactacacgt actcgaccac gatcgacgac cgcgacaccc tcaccgacat gtcggtcaac
     2281 cagaacatct gggccgatgg cgacgtcacc cagctcttcg acaacgacgc gatcatcgcc
     2341 tcgggcgacg gcgcgatcgc tgccggtggc agcgtggccg tggggtcgtt caacgaggac
     2401 tcgaacaaca ccgacaactc ggtggagatc gaggactcgt tcaacaccga caactcgctc
     2461 gagctggacg tcgagatcga ggactcgttc aacgacaatt cggacaactc cacctccgac
     2521 gacgacggca tcgacaatga cggtggcgac aacgacggca tcgacgtcga caactcgggc
     2581 aacgacaact ccgacaactc ggacaactcc gacgacgacg gcatcgacaa tgacggtggc
     2641 gacaacgacg gcatcgacgt cgacaactcc ggcaacgaga ctgacaactc ccaggagaac
     2701 gacaactcgg tcgacaactc ccaggagaac gacaactcgg tcgacaactc ccaggagaac
     2761 gacaactcgg tcgacaactc cagcgaggac gagaactcga tcgaggtcga gttcgagggg
     2821 tcgttcaacg acgacaactc cgacgacgac ggcttcgacg tcgacaactc cggtaacgac
     2881 aactcgtccg acaactccga cgacgacggc ttcgacgtcg acaactccgg caacaccgac
     2941 gacaactccg acgacgacgg cgttgacctt ctctgacgga ggcaacagac ccggccgcga
     3001 cctgcggctg aggtgatcga acgaacggcc cggtgggtag cccccgccgg gccgttcgcg
     3061 cgccccgctg cccgggtcgc aggtccgaaa ggaggaatgg gggcctttcc ggttgctatg
     3121 gtcgcgccct gactttgatc aggccgacgg gcgtcgaggt cgcgcggggc tgtgacctgc
     3181 ggtgttgtgc gtgagtgtgg tcgttttcac gcactaaggg ggctggttta gggtccgtgt
     3241 gtggcgttca tcaggcgggt gcggaccggc tcgggtgcga ccgccgtgca gatcgcggag
     3301 tacgccaacg ggcgtcagcg gattgtcgag cacgtcgggt cggcgcacac cgaggccgag
     3361 ctgggggtgc tgctggccag ggcttgtgag ctgctcgagg atcccgccca gggggcgctg
     3421 gacctggcgg tggaaccgtc ggtgccggtc gcctcgatcg tcgccgcgcc cgcggacgct
     3481 gccctgttcg acccctcgcc cgtggctgcg cctggccggg ccggcccggg caaggtggtg
     3541 ggtacacaca gccggctgct gttcgacgcg ctggccggcg tgtacgccgc gctggggttc
     3601 gatgcggtcg gggacgcggt gttccgggac ttggtgatcg cccgggtggt ggagccgacg
     3661 tcgctgctgg acgttgctcg ggtcctgacg gacctgggca gggcaccagc gagctacccg
     3721 acgatgcggc gcacgttggc tcgcgcccac aagcgtggat atcgagactt ggtcgcgaag
     3781 gcctgcttcg agcacgcgtc cacccgcggt gacatctccc tggtcctcta tggcgtgacg
     3841 acgttgtact tcgaggcgga gaaggaggac gacctgcgca aggtcgggta ctccaattat
     3901 gccgaggtcg tggttatgcc gaggtcgtcc tgttggggcg gatgttcgtg ctggtcaggg
     3961 gctcgggagg gtgaggctcg cgcggcataa ctctgcctgt ggagggtcat agccgttcga
     4021 ggaagtcgat gaggtcgtcg ccgggccggt agcgcccggg tgtcgtgttc gccgcgggga
     4081 cgcgggacag ggctcgctcc ttgatcgtca tgtcggcgtg caagtagatg tgcgtggttg
     4141 tcgcgttttg gtggccgagc cacagggcga tcacggaggt gtcgacgccg gcgctcatca
     4201 gcgccatcgc cgcggtgtgc cgcagggtgt gcggggtgac gtgtttggag gcgaccgatg
     4261 ggcactcgag cgcggcggtc gccgcgtgct tggcgacgag ccgttggacg gcgtcggccg
     4321 acatccgggt tccacttcgt gttgggaacg ctgggctgct gggtgtcggg ccggcctggg
     4381 gtagccacgc gagcagcacg gcggttgtct ggcgggtcag tggggtggcg cgttctcgtc
     4441 ggcccttgcc ggtggtgtgc acggcggcgc cggggctcgt ggtgatgatg tcctggatcg
     4501 tcagcccggt cagctcggac aggcggagcc cggtctggac cgcggtcagc agcagggcgt
     4561 ggtcgcgccg tccgtgccag gtcgtgcggt ccggagccgc cagcagggcg tcggtctccg
     4621 ggccggtcag gtaggacacg atggctcggt cgtgacggcg ttgcgggatc gccaggacct
     4681 gactggccgt gtgggcccgc tcggggatga gcggcagcgc gtaccggtac agcgagtgga
     4741 tcgcggccag gcgagcgttg cgggtcgctg tgctgttgcc ccgttcggtg tcgaggtggt
     4801 ccaggaacgc gccgaccatg acggcgtcga ggtcgtcgat ggtgaggcgg gctggggcct
     4861 tcttcgtcgc ctgcgccgcg aacagcagca gcagcttgat cgtgtcccgg taggcggtga
     4921 tggtctgcgg gctggcctgc agctgccgga ccagtcgctc ggtgaagaac ccttgaagca
     4981 gtgatggcag ggtcggcgcg gtcatggcgt cctctccccg gtcggcgggc tcgcttgcgg
     5041 ctcgaggcgg gtggctgcgt gggcgagtag ttcggggacg gcgtgcaggt accagtaggt
     5101 cgaggcggga tcgacgtgac cgagccaggt cgacagcagc ggcaggcgcg cttggacgtc
     5161 gtgaccgtcg cggtaccagc cctggatcgt ggccacggtg taggtgtgtc tcagatcgtg
     5221 caacctgggc ggtctgtgtc cgggcttggc ggtgacgccg gcagcggcga cgacctgtct
     5281 gaaggtggca cccaaccggt ccgggttgat cgcctcgcgg cggctgttga cgaacacggc
     5341 cgggcaggct cgtgccgtgg tcccgaccca atcccggcgg acctgcaggt agtcggccag
     5401 gacggtggcc gtcgtcgggt ggatgaacac gagcctggac ttgccgaact tcgtatccag
     5461 gacctggagc acgccgcctg ccaggtcgac gtcgtcgacg ctgaggcggc acgcctcacc
     5521 gggacgcatc cccgtggccg cgagcagccc gatcaacgtg cgccacgtga tcgccttgaa
     5581 ccgcggcgac aaggtgtccg ctgccaccac cagggccagg acctgcgcct cggtgaacac
     5641 gttcggctct ctcggctggt atcgccggtt gccgatcccc tcggcaggga tctgcgtgga
     5701 cgggtccagc gcgtgctggt gccgtgcgaa cacgcgcacc gcgtccatcc ggcgcgcgat
     5761 cagggcgtca gtgacgggga ccttgatcgg cgtggtggcc cacgccacgg cgaggtcgtt
     5821 ccggacatgg ctggcaccac gcgcctcgca gaacgtgacg aagttcgaca gcagcttgct
     5881 cagtccctcg accttgaagc ccatcgcccg gcgggtggcc agatagtcgg tgacgtcctc
     5941 gcgcagcgtg ctcatgatcg gccccctggc cacgggagag ccaggctgcg agcggccgcg
     6001 tcaggcagtg cagggccagg cgcggtcccc ggccacggcc gggccagcgg gcgcagggca
     6061 acctcgtcga ccttggcata gatcgcggtc gacctcgcgc tgcgatgccg cagcacctga
     6121 cccacctccg gcaacgacgc cccggcccgc agcatcgcgg tggccaggct gtgacgtagc
     6181 tggtgcgcac cgatccgtgc cagcccggcc cgctggcagg cactgcccac gacgtgtccg
     6241 atcgcgccgg ccgacagcgg ctggccggcc ggccgcagcg tggtgaacac cgatcgcgtg
     6301 tcgcactcgg gccgcccatc ggtcaaccac gcgacgatcg cctctcccgg ctcgaccggc
     6361 agaggcaggc gttcgacccg gccgcccttg ccgaccacgg tcagctcgcc ggacctccag
     6421 tcgatgtcgt ccaggcgaag gcctgcgacc tcgcctcgcc gcagcccgag ccgggccagg
     6481 accagcaaga tcgcgtagtc acgccgaccc gcttccgtga accgcaaccg cgcggcaact
     6541 gtcagaagtc gctccaggtc ggcgcccggc accgccttcg gcagcgatgc cagatgccat
     6601 cccgaggacg ccggaacggc accccacaat tcggtcgagg ttcgacccgt cgcgtgagcg
     6661 aatctcagga acgatcgcac cgacctggcc atcgacttcg cggagttcgt gttgcgaccg
     6721 cgacagaact cgaccatgaa cgcggtgacg gtgccggcgt ccagcagacc caccgacacc
     6781 tgcgtcgact cgggcagcgc gtccaggaac tgtctcgcat tccagcagta gttgtgaacg
     6841 gtcaccggtg ccagtccccg ctggcgatcc aaccacgctg cgaactcgac gagcagctcg
     6901 ctcaccgaag ccgagccgcc gcggacatcg acactgctta cccggtccat ttctcctcct
     6961 cctgatgggc caaccagagc accaccagac aggcacaaca ccgagtcccc cgcgagggtt
     7021 atgccgcgcg ggcctgaccc tcgcgaaccg ctgaccagca cgaacatccg ccccaacagg
     7081 acgacctcgg cataaccacg acctcggcat aattggagtt atgccgacgt cggcataact
     7141 ccaaggaacg ccgtgtggac ccgcagatca ttgtcgggct gctggtggac cgggccgggt
     7201 tcccgctgga gatcggctgc tgggagggca acaaggccga gaccgcgacg atgctcccga
     7261 t
//
LOCUS       DAOMZY010000165         7257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_9654_length_7257_cov_5.631491, whole genome shotgun sequence.
ACCESSION   DAOMZY010000165 DAOMZY010000000
VERSION     DAOMZY010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7257
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9654_length_7257_cov_5.631491"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PLK64_10070"
     CDS             complement(<1..408)
                     /locus_tag="PLK64_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPV80136.1"
                     /translation="MRRFGEVLTTRGRAFCSAGITLMVCGYVLGFRDLTRVGVLLLGL
                     PLLAGLLARRRPPGLVVTRTSDPVQISAGEATTVTTTVANGGHRSTPLMLAEEQLDHV
                     LGERPRVLLGSLGAGERRALTYAVRPPLRGRHLL"
     gene            complement(442..1410)
                     /locus_tag="PLK64_10075"
     CDS             complement(442..1410)
                     /locus_tag="PLK64_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPV80137.1"
                     /translation="MVRTTPEALAELRAVTSRIASAMESVIEGKPAVVRTAITVLLAG
                     GHLLIEDVPGVGKTMLAKALARSIDCSVRRIQFTPDLLPSDITGVSVFNQDLRDFEFR
                     PGAIFANVVVGDEINRASPKTQSALLECMEESQVTVDGTTYSLQRPFIVMATQNPIEM
                     EGTYPLPEAQRDRFMARISMGYPSTAAEVEMLDLHGGRSPLDTLVPVTDAQTVARLVL
                     VTQDVYASPAVRQYVVDLANATRTSPAVRLGASPRAALHVLRAARAHAALEARDHVLP
                     DDVQRVAPAVLGHRLMLSGEATLAHRSPAEVLAEILRSTPVPIGRR"
     gene            1943..2383
                     /gene="mraZ"
                     /locus_tag="PLK64_10080"
     CDS             1943..2383
                     /gene="mraZ"
                     /locus_tag="PLK64_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HPV80138.1"
                     /translation="MPTPFLGTHTPKLDDKGRLILPAKFREPLAEGLVMAKGQERCIV
                     VYPMAEFYRFAAQMQTAPSSVSGVRDYTRVLASSASPEVPDKQGRITIPPMLREYAAL
                     GRDLAVIGNISRVEIWQLDAWNRYLADKEPGYASQSEEVMPGRF"
     gene            2637..3626
                     /gene="rsmH"
                     /locus_tag="PLK64_10085"
     CDS             2637..3626
                     /gene="rsmH"
                     /locus_tag="PLK64_10085"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPV80139.1"
                     /translation="MTTSPERATSDRHVPVLRDRVLELLAPALAAEGAVYVDGTLGMG
                     GHAEGVLQAFPHARLIGVDRDPQALALAGQRLAPFADRITLVHAVYDEFAQVVDALGI
                     PTVDAALFDLGVSSLHLDETDRGFAYRVDAPLDMRMDQTRGMTAADVLNGYEPADLER
                     ILRDFGEERYARSIVRAIVADRAKEPYTTSGPLVDMLRRVVPGASQRSGGHPAKRTFQ
                     ALRIEVNGELDAWRRALPAAVDRLRVGGRVAVLSYHSLEDRITKRCLAEGATSRTPIG
                     MPVERPQDAAYLKLLTRGAEVPTDAEIHDNPRAASARLRAAERTRPTTQGEHR"
     gene            3623..4225
                     /locus_tag="PLK64_10090"
     CDS             3623..4225
                     /locus_tag="PLK64_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80140.1"
                     /translation="MSQASALRHAASTSGRPAGRPSGPGARLRVVPAAIERTGTGKFA
                     AICMVLLTVGLLALLLLNTQLAQGSFRLHDLQVASGTLTDQEHELTRALDAERNPGSL
                     AKRALDMGMVPATSMAFIRLEDGVILGVAEPAAAKVLTVVNAPKTQPSAPAPVAPDPA
                     ATPAPAATPAPTATPAPADAAASDPEVPDGAPVTATVPAG"
     gene            4521..6296
                     /locus_tag="PLK64_10095"
     CDS             4521..6296
                     /locus_tag="PLK64_10095"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPV80141.1"
                     /translation="MWVAVLFVLSIFGAQLVRVQAFDASGVQLAALNKRLNSVVVPAL
                     RGRIVDTKGTVLAASVERRTITVNQNAVATYERVIEGKRVKVGVVGAAERLAPLLGMT
                     ADELVKKLMGTSGYQVIAKGVSPLVWREISAMGIPGILSEVTFEREYPSGMTGAPIVG
                     FLGDDGSVAGGVEQMMQAQLAGTPGKEIYEQARDGKVIPWAQRLSQPAVDGQDVRLTI
                     DADIQWFAQNTIGNLVHAMGAKSGTIVVMEAKTGKLRAVAPYPSFDPTDLAHTKASAL
                     GNKAFQEVYEPGSTAKVITIAAALEENVVEPMTGVIVPNRLRRADTTFKDAHDHPTLN
                     LTVAGVLAKSSNIGTILATESMPAATLEAYQRSFGLGTKSGIGYPAESAGLLRPAAIQ
                     TGSERYTTMFGQGMSVTAVQAAGVFQTIANGGVRVPPSLIEGTVAEDGTFAASTAPQG
                     VRAVSQQTSTEVSQMLEEVVGPGGTARHVAIPGYRIAGKTGTANRVADSGAGYSGYTT
                     SFIGYAPAEDPQFVVAVTLQNPTAGVPSGGSQCGPAFKDVMTYVLQAYQVPPTGAASA
                     EVPLVPAEPLVPGAPGVISDRKSTG"
     gene            6307..>7257
                     /gene="murE"
                     /locus_tag="PLK64_10100"
     CDS             6307..>7257
                     /gene="murE"
                     /locus_tag="PLK64_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl-tripeptide synthetase"
                     /protein_id="HPV80142.1"
                     /translation="MHSHRPRSLPPVALETLADRAGATVVGGGSAQGVLVTGVSLDSR
                     TVRPGDLYAALPGAHAHGATFVAGAVAAGAVAVLTDPEGAPLAAIGLGVPILLVEAPR
                     AVLGDLAALVYGHPADAMRLIGVTGTNGKTTTAYLIESALRGLGARTGLIGTVETRVG
                     DERLTSRRTTPEAPDLHAILAVMREAGTDSVVMEVSSHALAQHRVDGVVYDVALFTNL
                     SQDHLDYHAGMADYFAAKAALFSPERAQRGVVCIDDEWGVALAQRRGIPLVTVSSRRP
                     ADWTLTAGEGPEVTLVGPGEQQLLLHCHLPGTFNLVNTAMA"
BASE COUNT         1065 a         2678 c         2501 g         1013 t
ORIGIN      
        1 gagcaggtga cggccgcgca gtggtgggcg gacggcatac gtgagagcgc ggcgctcgcc
       61 cgcgccgagc gagccgagca gcacccgcgg tcgttccccg aggacgtggt cgagttgttc
      121 ctcggcgagc atgagcgggg ttgaccggtg accgccgttg gcgaccgtcg tggtcaccgt
      181 ggtggcctcg cccgcgctga tctgcacggg gtcggaggtc ctggtgacga ccaaccccgg
      241 cgggcggcga cgggcgagca gaccggccag cagcggcaac ccgagcagga gcacgccgac
      301 ccgggtgagg tcacggaagc cgagcacata gccgcagacc atgagggtga tccccgccga
      361 gcagaaggct cgcccgcggg tggtgagtac ctcgccgaaa cgtcgcatcg tcctcgcctg
      421 gccgtatgcc gcgccgcgcg gtcagcgccg cccgatgggg acgggggtcg accggaggat
      481 ctcggcgagc acctccgccg ggctccggtg ggccaacgtg gcctcgcccg acagcatgag
      541 tcggtggccg agaacggccg gggccacccg ctgtacgtcg tcgggcagga cgtggtcgcg
      601 agcctccaaa gcggcgtggg cgcgcgcggc ccgcaggacg tgcagagccg ctcgcggcga
      661 ggcacccagc cgcacggcgg gggaggtgcg cgtcgcgttg gcgaggtcga cgacgtattg
      721 ccggacggcg ggggaggcgt agacgtcctg cgtgacgagg accaaccggg cgaccgtctg
      781 ggcgtcggtg actgggacga gggtgtccaa cggcgagcga cccccgtgca ggtcgagcat
      841 ctcgacctcg gcggccgtcg acgggtaacc catcgagatc cgcgccatga accggtcgcg
      901 ctgggcctcg ggcagcgggt aggtcccctc catctcgatg gggttctggg tcgccatgac
      961 gatgaacggc cgctgcagcg agtaggtcgt gccgtcgacc gtgacctggc tctcctccat
     1021 gcactccagc agtgccgact gggtcttggg ggaggcccgg ttgatctcgt cgcccacgac
     1081 gacgttggcg aagatggcgc ccgggcggaa ctcgaagtcg cgcaggtcct ggttgaagac
     1141 gctcacgccg gtgatgtcgc tgggcagcag gtcgggggtg aactggatcc ggcgaaccga
     1201 gcagtcgatc gaccgggcca aggccttggc gagcatcgtc ttgccgacgc cggggacgtc
     1261 ctcgatgagc aggtgaccgc cggcgagcag gacggtgatc gcggtgcgga ccaccgccgg
     1321 cttgccctcg atgaccgact ccatcgcact ggcgatgcgc gaggtcacgg cgcggagctc
     1381 cgccagggct tcaggggtcg tgcgcaccac ccccgctgcg gtcgggtcgg tgtcgggtcg
     1441 ggtccgggcg tcggtcacgg ttgttcctcc gggtgctctg ccgtagcgac ggccccgccg
     1501 ggtgggcggt gccgtcctcc acttcgctcc accgggggcc tggctgggca ccccagtccg
     1561 gcgagcatag gtcggctcgc tttgaaatgc ggccgaaccc gggcatcggg tgagtaacgg
     1621 accggttgcg agggggtccg gcgcgggtcc gtgcgggtcg ccgccctcct gggtatgccg
     1681 tgcaccgcac ccgagcggct cctcgcgggg tggctgcgcg gcataccctc cacttccctc
     1741 caccctcctg acctgcggcg ttatgggtcc taccaagaaa atcgggaaat ctggggcgtc
     1801 aagtggaggg aagtggagta tcgtggggcg ccaaggagcg tggtggtgag gtctcatcgc
     1861 ccgccgggtc gtgtgggtgg gcccctcagg gggtctggga gggaaggggt ggtgcgccgt
     1921 ggtcgacgtc cccatcgccc cgctgccgac cccgttcctc ggcacgcaca ccccgaagct
     1981 ggacgacaag gggcgactga tcctgccggc gaagttccgc gagccgctgg ccgagggact
     2041 ggtcatggcc aagggccaag agcgctgcat cgtcgtctac ccgatggccg agttctaccg
     2101 gttcgccgcg cagatgcaga cggcaccgag ctcggtgtcc ggggtccgcg actacacgcg
     2161 cgtgctcgcc tccagcgcct cgccggaggt ccccgacaaa cagggccgga tcaccatccc
     2221 gccgatgctg cgcgagtatg ccgcgctcgg ccgggacctg gccgtcatcg gcaacatcag
     2281 ccgcgtcgag atctggcagt tggacgcctg gaaccgctac ctggccgaca aggagccggg
     2341 ctatgcctcg cagagcgaag aggtgatgcc cgggaggttc tgaccggctg accacctgcg
     2401 cccaggtctc cagccgcttc cgtcccgaca cgacttcccc cgtggcgggc cgacgcggtt
     2461 cgagaaccat ccgaagcgga tggggacctg agcgcagggc catatcggcc ccgacccacc
     2521 cctccgtacc caccccaccc gtccccaccc tccccgcctc gtccccgcct cgtccccgcc
     2581 gcatcacctc ccagcccccc cccgtcccac tgagccacca gacctcggag ttcgcgttga
     2641 ccacctcgcc agagcgcgcc acatcggacc ggcatgtccc ggtcctgcgc gaccgggtgc
     2701 tggagttgct cgcgcccgcg ctggccgcag agggcgccgt ctatgtcgac gggaccctcg
     2761 ggatgggtgg gcacgccgag ggcgtcctgc aggccttccc ccacgcgcga ctcatcggcg
     2821 tcgaccgcga cccgcaggcg ttggctctgg ccgggcaacg gttggccccc ttcgccgacc
     2881 ggatcaccct cgtccacgcg gtctacgacg agttcgccca ggtcgtcgac gcgttgggca
     2941 tcccgacggt ggacgcggcg ctgttcgacc tcggggtcag ctcattgcac ctggacgaga
     3001 ccgaccgtgg gttcgcctac cgcgtcgacg cgccgctgga catgcggatg gaccagaccc
     3061 gcggcatgac cgccgccgac gtcctcaacg gctacgagcc ggccgacctc gagcggattc
     3121 tgcgcgactt cggcgaggag cgctacgccc gctcgatcgt ccgcgcgatc gtcgccgacc
     3181 gcgccaagga gccttacacg acgtccgggc ccctggtcga catgctccgc cgggtcgtgc
     3241 cgggggcgtc gcagcgcagc ggcggccacc cagccaaacg gaccttccag gcgctgcgca
     3301 tcgaggtcaa cggcgagttg gacgcctggc gccgagccct cccggccgct gtcgatcgcc
     3361 tgcgcgtggg tggtcgagtc gccgtcctct cctaccactc gctcgaagac cggatcacca
     3421 agcgctgcct cgccgaaggc gcgacctcgc gcacaccgat cggtatgccg gtcgagcggc
     3481 cgcaggacgc ggcatacctc aagctcctca cccgcggcgc cgaggtgccc accgacgcgg
     3541 agatccacga caacccgcgc gcagcctcgg ctcgtctccg cgccgccgaa cggacccgtc
     3601 cgaccaccca gggggaacac cgatgagtca ggccagtgcg cttcgacacg ccgccagcac
     3661 cagcggccgg ccagcggggc gacccagcgg tccgggcgcc cgcctgcggg tcgtgcctgc
     3721 cgccatcgag cggaccggca ccgggaagtt cgcggcgatc tgcatggtcc tgctcaccgt
     3781 cggcctcctc gcgctgctgc tgctcaacac ccagctcgcc caggggtcct tccggctgca
     3841 cgatctccag gtcgcctctg gcaccctgac cgaccaggag cacgagctca ctcgcgctct
     3901 cgacgccgaa cgcaaccccg ggtcgctggc caagcgcgcc ctcgacatgg gtatggtccc
     3961 cgcgacgtcg atggccttca tccggctcga ggacggggtc atcctcgggg tcgccgagcc
     4021 ggcggcagcc aaggtgctca ccgtcgtcaa cgcaccgaag actcaaccca gcgctcccgc
     4081 ccccgtcgcc ccggatccgg ccgcgacccc tgcgccggcc gcgaccccgg cgccgaccgc
     4141 gaccccggct cctgccgatg ccgcggcgtc cgatcccgag gtccccgacg gcgcccccgt
     4201 gaccgccacg gttccggcgg ggtgacgaca tgacgttgcc gccccgtcag ggtcaacccc
     4261 ccaccaatag acgccgcccg cccgacccca gcccacaccg cgtgcccgac cccagcgcgc
     4321 gccgcgtgcc cgaccccagc gcgcgccgcg cgcccgaccc cacggcccga ccgcgcgccc
     4381 cgcgcgccgc ggcaccgacg cggcggcccg ccgcgccccc gcggcgccct gcggcgcccc
     4441 cacgtccgcc gcgatccgcg cgtccgcggc ggatccgtcg gcacctgagc gtcgggcaca
     4501 cgacgtggcg cgtgcgcacc atgtgggtcg ctgtcctctt cgtcctgtcg atcttcggcg
     4561 cccaactcgt gcgggtgcag gcgttcgacg cctccggcgt gcaactagcc gccctcaaca
     4621 agcggctcaa ctcggtggtc gtccctgcgc tgcgcggtcg tatcgtcgac accaagggga
     4681 ccgtcctggc cgcgagcgtc gagcggcgca cgatcaccgt caaccagaac gccgtcgcca
     4741 cctacgagcg ggtcatcgag gggaagcggg tcaaggtggg cgtcgtcggc gcggccgagc
     4801 gcctggcacc cctgttgggg atgacggccg acgaactcgt caagaagctc atgggcacca
     4861 gtggctacca ggtgatcgcc aagggcgtgt caccactggt gtggcgggag atcagcgcca
     4921 tggggatccc cgggatcctc agcgaggtga ccttcgagcg ggagtacccc tcggggatga
     4981 cgggcgcgcc catcgtcggc ttcctcggcg atgacggcag cgtggccggt ggcgtcgagc
     5041 agatgatgca ggcccagctt gccggcacgc cgggcaagga gatctacgag caggcgcgcg
     5101 acggcaaggt cattccatgg gcccaacggc tctcgcagcc tgccgtcgac ggccaggatg
     5161 tgcgactcac catcgacgcc gacatccaat ggttcgccca gaacaccatc ggcaacctcg
     5221 tgcacgcgat gggtgccaag tcgggcacca tcgtcgtgat ggaggccaag accggcaagt
     5281 tgcgggccgt tgcgccctac ccgagtttcg atccgaccga cctggcccac accaaggcat
     5341 ccgcgctagg caacaaggcc ttccaggagg tctatgagcc ggggtcgacg gcgaaggtca
     5401 tcaccatcgc ggccgcattg gaagagaacg tcgtcgagcc gatgacgggg gtcatcgtcc
     5461 ccaaccggct gcggcgtgcg gacacgacct tcaaggacgc ccacgatcac ccgaccctga
     5521 acctcaccgt ggccggggtg ctcgcgaagt cgagcaacat cggcacgatt ctggcgacgg
     5581 agagtatgcc ggccgccacc ctcgaggcct accagcgctc gttcgggctg gggaccaaga
     5641 gcgggatcgg ctaccccgcc gagtcggccg gcctgttgcg tcccgccgcg atccagaccg
     5701 ggtcggagcg ctacacgacg atgttcggcc agggcatgtc cgtcaccgct gtccaggcag
     5761 ccggcgtgtt tcagaccatc gccaacggcg gcgtccgtgt gccgccgagc ctcatcgaag
     5821 gcacggtcgc cgaggacgga accttcgcgg cgagcacggc cccgcaaggg gtgcgcgcgg
     5881 tgtcccaaca gacgtccacc gaggtgagcc aaatgctcga ggaggtcgtc ggaccgggcg
     5941 gcaccgcccg gcacgtggcc atcccgggtt accggatcgc cggcaagacg ggaacggcca
     6001 accgggtcgc cgacagcggc gccggctaca gcggctacac cacctccttc atcggctacg
     6061 caccggccga ggacccgcag ttcgtcgtcg cggtcaccct ccagaacccc accgccggcg
     6121 ttcccagcgg tggcagccag tgcgggcccg cgttcaagga cgtcatgacc tacgtgctgc
     6181 aggcctacca ggtgcctccg acgggtgcgg ccagcgccga ggtccccctc gtccccgctg
     6241 agcccctcgt gcctggcgca cccggcgtga tcagcgaccg caagtccacc ggctagcctg
     6301 atcgccgtgc attcccaccg gccccgatcg cttccgccgg tcgcccttga gacgctcgcc
     6361 gaccgtgcgg gcgccacggt cgtcggcggg gggagcgcgc agggggtcct cgtcaccggg
     6421 gtgagtctcg actcgcgcac cgtccgaccc ggcgacctgt atgccgccct tccgggcgcc
     6481 cacgcgcatg gcgccacctt cgtggcgggc gcggttgcgg cgggcgccgt cgccgttctt
     6541 accgacccgg agggggcgcc gctggcagcc attggccttg gcgtcccgat cctgctcgtc
     6601 gaggcgccgc gcgcggtcct cggcgaccta gccgcactgg tctatggcca tcccgcggac
     6661 gcgatgcggc tgatcggcgt caccggcacg aacggcaaaa cgaccaccgc gtatctcatc
     6721 gagtcggcgt tgcggggtct cggagcccgg actggactca tcggcacggt cgagacccgg
     6781 gtcggtgacg aacggttgac ctctcgtcgc accactcctg aggcacccga cctgcacgcg
     6841 atcctcgccg tcatgcgtga ggcggggacc gactcggtcg tcatggaggt gtccagccat
     6901 gcgttggccc agcaccgggt cgacggggtc gtctatgacg tcgccctctt caccaacctg
     6961 tcccaggacc acctcgacta ccacgcgggg atggccgact acttcgcagc caaggcggcg
     7021 ttgttctccc cggagcgcgc gcagcgcggg gtcgtctgca tcgacgacga gtggggcgtg
     7081 gcgctcgcgc aacgacgcgg catacccctg gtcaccgtct cctcgcgtcg accggccgac
     7141 tggacgctca cggcggggga ggggccggag gtaaccctcg tcggccccgg tgagcagcag
     7201 ttgctcctgc actgtcacct ccccgggacg ttcaacctcg tcaacaccgc catggcc
//
LOCUS       DAOMZY010000166         7249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_9665_length_7249_cov_5.608007, whole genome shotgun sequence.
ACCESSION   DAOMZY010000166 DAOMZY010000000
VERSION     DAOMZY010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7249
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9665_length_7249_cov_5.608007"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="PLK64_10105"
     CDS             complement(<1..796)
                     /locus_tag="PLK64_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80143.1"
                     /translation="MSTVDRSAARARVDAAYARLTEVERGWTRIVHDPATYTRDVPYA
                     ALAGLRDRHGVVPVAEPALPGWPEGTGYWLVLRHADVDRVLKDPATFSSSLGGTQMRD
                     PRTEDDLAYVRRMMLNMDPPEHSRLRRLLAKSFTPKAIGALEAQIQGHVTGILDQALD
                     STPTSRQGACDFAKDVSADLPLLTLADVMGVPAPDRWLMFDWANRVIGWQDPDYRVSA
                     AFDPSGATELGRAAYALRPSPGADGLMPDPRTREGMPDLYAYAHALG"
     gene            complement(813..1568)
                     /locus_tag="PLK64_10110"
     CDS             complement(813..1568)
                     /locus_tag="PLK64_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPV80144.1"
                     /translation="MTIPCITVGSGEHHVFVLHGWFGSADGWGLFPTYLDGERFSYHF
                     TDNRGYGARMDERGHYSLDEVADDVLALADSLDAQTFSLIGHSMGGAEVLRILAKAPG
                     RVRKLVGLTPVSASPTPFDEAGHDLFWGAAEDPGKRFGIIDFTTGNRNTATWVQQVVD
                     HSLSHSTTEGFAGALEAWAGPDFLAEIAGTQTPMLVIAGEHDPALGEATVRQTWEPHF
                     PQCEIHVMANAGHYPMFETPVQLATVIERFLAD"
     gene            complement(1608..3848)
                     /locus_tag="PLK64_10115"
     CDS             complement(1608..3848)
                     /locus_tag="PLK64_10115"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019482618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPV80145.1"
                     /translation="MTAGPDTQAPPSELRQTQLVVGGMTCASCSARVEKKLGKLTGVT
                     AAVNYATGKAFVVHPGGLAVADLVAAVESAGYTATPAPPSIPGSQPGPGEGGEPDADD
                     EHAAYLRQRLLVALGLSVPAIAISMVPALAFPGWPWVVLALTLPVVTWCAWPFHRAAW
                     VNLRHGATTMDTLVSGGVLAAFGWSLWMLLTMGAHGHFYFETAAAITTFLLAGRYAEA
                     KAKRRSGAALRALLHLGAKDVAVLRGDVEVRIPLGELLVGDEFVVRPGEKVATDGVIV
                     DGASAVDASLLTGESVPVEVGVGDPVTGATVNTSGRLRVRATRVGGDTQLAQMARLVE
                     SAQSGKAAVQRLADRVSAVFVPVVLVLAALTLIGWLVTGHSAESAFTAAVAVLVIACP
                     CALGLATPTALLVGTGRGAQLGILIKGPEVLESTRRVDTVVLDKTGTVTTGRMAVTGV
                     YAAPGQSPERVLALAGAVESGSEHPIARAVTSAASAPGLRLSEFRNHGGLGVEGVVDG
                     MPVRVGRLSFVTSAPATGQGVDVSLSEAAEEAMAQGQTPVWVGWDGTAQGVVVVADTV
                     KPSSARAVELFRGLGLQPVLLTGDNARAAAAVARHTGIAQEDVRAEVLPAEKVAVVAA
                     LQAQGKVVAMVGDGVNDAAALAQADLGIAIGTGSDVAIEASDLTLVRGDLVSAVDAVR
                     LARATLRTIKGNLFWAFAYNVAAIPLAASGRLTPMIAGAAMAFSSVFVVTNSLRLRRF
                     KPSISA"
     gene            complement(3927..4148)
                     /locus_tag="PLK64_10120"
     CDS             complement(3927..4148)
                     /locus_tag="PLK64_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HPV80146.1"
                     /translation="MATQSFQVTGMTCGHCAGHVTAELKSVAGVSDVTVDLVAGGTST
                     VHVDADHELTDAEVTAALDEAGNYALAGR"
     gene            complement(4238..4891)
                     /locus_tag="PLK64_10125"
     CDS             complement(4238..4891)
                     /locus_tag="PLK64_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80147.1"
                     /translation="MSTLDTTSATTHRRSALLAPVGVLAVVAGVIGWMLPDRTVGTGA
                     MDGMSMTHYMGLLAVNQPWNLILFMAIPVILAETLAITELVLLFRSDPPTWVRSLSRW
                     AGLIAGPVMVLVLVHLLKNAVVPLTSGGGWRGAADVIAVLTYLLAGLPLIGITLVEVG
                     AIGTDARDARKWHAIFVGVFLVLAHVAMIFGMLDPTVLGWGATHDMPGGGTMPGMNH"
     gene            complement(5214..6611)
                     /locus_tag="PLK64_10130"
     CDS             complement(5214..6611)
                     /locus_tag="PLK64_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV80148.1"
                     /translation="MSSTPTTPRSGEWLDAWDPENEATWDKALAWKTLWITTFTLTMA
                     FVAWFLPSAIVPKLNALGYTFSKEQLYWLAAIPGLSGGLMRIVWMILPPIMGTRRMVA
                     LTTALLFFPVLGWGVRVQSPTAPYWELLVLAFLAGIGGGAFSGFMPSTSYFFPRRLQG
                     TALGLQAGIGNFGVSLVQLMTPWLIGFGMVGFLGASQQMKVPGKPDVTVWYQNAAFFY
                     IPFIIVGTIIAWTMLKSVPIKANIRQQFDIFRTPDTWWMTLLYIMTFGTFSGLSAQFG
                     LLMTNLYGAGNAAIVSGSGSTATLLVAGYAVPDAVKYVFLGPLVGAGARILFSPLTDR
                     MGGARWTLVSGIGLVGSIIYTIGGLVPDTSSAEALQAGFGRFLWGMLAIFLFAGIGNA
                     STFKQMPMIFERRQAGGVIGWTGAIAAFGPFLFGVGLTLMSPTAFYWIGAAFAVLCIG
                     ITWVRYARPGAPKVS"
     gene            complement(6691..>7249)
                     /locus_tag="PLK64_10135"
     CDS             complement(6691..>7249)
                     /locus_tag="PLK64_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV80149.1"
                     /translation="WPAALLTATFIFPASLLRPLGGWVSDRVGARRVMYWTFGVMLVT
                     TGILMMPNGHIVVSHPDGSQTTHLGYQIGLPTFAILVFFLGVAMGIGKAAVYKHIPEY
                     FPDNVGSVGGLVGMLGGLGGFFLPPMFAYTKAWTGFPSSTFACLFIVTAICAVWMHWT
                     VVHMLHSGSPELADHFDTEHVSTRG"
BASE COUNT         1246 a         2503 c         2499 g         1001 t
ORIGIN      
        1 tcccgagcgc gtgcgcgtag gcatagaggt caggcatgcc ctcacgggtg cgggggtcgg
       61 gcatgaggcc gtccgctccg ggcgaggggc gcagcgcgta tgccgcccgc ccgagttcgg
      121 tcgccccgct cgggtcgaag gcggccgaga cgcggtagtc ggggtcctgc cagccaatga
      181 cccggttggc ccagtcgaac atcagccagc ggtccggcgc cggcaccccc atgacgtcgg
      241 ccagggtgag cagcggcagg tcggccgaca cgtccttggc gaagtcgcac gcaccctgcc
      301 tgctggtcgg cgtgctgtcc agggcctggt caaggatgcc cgtgacatgc ccctggatct
      361 gagcctccag cgcgccgatc gccttggggg tgaacgactt tgccaacagc cgccgcagcc
      421 gggagtgctc gggcgggtcc atgttgagca tcatccggcg cacgtaggcc aggtcgtcct
      481 cggtccgcgg atcgcgcatc tgggtgccac cgagcgacga cgagaaggtc gccggatcct
      541 tgagcacccg gtcgacgtcg gcatggcgca gcaccagcca gtagcccgtc ccttccggcc
      601 agccgggcag ggccggctcg gcgaccggga cgacaccgtg tcggtcgcgc agacccgcga
      661 gggcggcata cgggacgtct cgggtgtagg tcgcggggtc gtggacgatg cgcgtccagc
      721 cccgctcgac ctcggtgagc cgcgcgtatg ccgcgtccac ccgcgcgcgg gcggctgacc
      781 gatcgacggt gctcacctgc ggcgggccgg ggtcagtcgg cgaggaagcg ctcgatgacg
      841 gtcgcgagtt ggaccggcgt ctcgaacatc gggtagtggc cggcattggc catgacgtgg
      901 atctcgcact gggggaagtg cggctcccac gtctgacgga ccgtcgcctc gccgagcgcc
      961 gggtcatgct cgccggcgat gacgagcatc ggagtctggg tgccggcgat ctcggcgagg
     1021 aaatccggtc cggcccatgc ctcgagcgcc ccggcgaacc cctccgtcgt cgagtggctc
     1081 agcgagtggt cgacgacttg ctggacccag gtggccgtgt tgcggttgcc ggtcgtgaag
     1141 tcgatgatcc cgaaccgctt gcccgggtct tccgcggcgc cccagaacag gtcgtgcccg
     1201 gcctcgtcga acggggtcgg ggaggcgctg acgggggtca gaccgaccag cttgcgcacg
     1261 cgccccggcg ccttcgcgag gatgcgcagg acctcagccc cacccatcga atgcccgatc
     1321 aacgaaaacg tctgcgcatc aagggaatcc gccagcgcca ggacatcgtc cgcgacctcg
     1381 tcgaggctgt agtggccgcg ctcgtccatc cgggcgccat agccgcggtt gtcggtgaag
     1441 tggtagctga accgctcgcc gtcgaggtag gtcgggaaga gaccccaccc gtcggccgag
     1501 ccgaaccagc cgtgcaggac gaagacgtgg tgctcgccgg aaccgacggt gatgcagggg
     1561 atcgtcatgc atcgaggcta ttcctcgccc gccccgggcg ggagacccta ggccgagata
     1621 gacggcttga agcggcgcag ccgaaggctg ttggtgacga cgaagaccga cgagaaggcc
     1681 atcgccgcgc cggcgatcat gggggtcagc cgaccggagg cggccaacgg gatggcagcc
     1741 acgttgtagg cgaacgccca gaacaggttc cccttgatcg tccgcagggt cgcccgggcc
     1801 agccgcaccg cgtcgacggc gctgaccagg tcgccccgga ccagcgtgag gtcggacgcc
     1861 tcgatggcca catcgctgcc ggtgccgatg gcgatcccga ggtcggcctg agccagcgcg
     1921 gccgcatcgt tgacgccgtc cccgaccatc gcgaccacct tgccctgcgc ctgcagtgcc
     1981 gcgacgacgg ccaccttctc ggccggcagc acctccgcgc gcacatcctc ctgcgcgatc
     2041 ccggtatgcc gtgccactgc ggctgccgct cgcgcgttgt cgccggtgag cagcaccggt
     2101 tgcaggccca ggcctcggaa gagctcgacg gcgcgggctg atgacggctt gacggtgtcg
     2161 gcgacgacga ccacaccctg ggccgttccg tcccagccga cccagaccgg ggtctggccc
     2221 tgggccatcg cctcctcggc agcctcactc aggctcacgt ccaccccctg cccggttgcg
     2281 ggcgcggagg tgacgaagga caagcggccg acgcggaccg gcataccgtc gaccacgccc
     2341 tccaccccga gcccgccgtg gttgcggaac tcgctcaacc gcaatccagg agcagacgcg
     2401 gccgaggtga cggcgcgggc gatggggtgt tcggacccgg actcgaccgc gccggccagc
     2461 gccagcaccc tctccgggga ctggccggga gcggcataca cacccgtgac ggccatccgc
     2521 ccggtggtga ccgtgcccgt cttgtccagg acaacggtgt cgacccgacg cgtggactca
     2581 aggacctcgg ggcccttgat gaggatgccc agttgcgcgc cgcgacccgt gccgacgagc
     2641 agtgcggtcg gcgttgcgag cccgagcgcg caggggcagg cgatgacgag caccgccacc
     2701 gccgcggtga aggccgactc ggcggaatgc ccggtcacca gccacccgat gagcgtgagt
     2761 gccgccaaca ccaacaccac cgggacgaac acggccgaca cccgatcagc gagccgctgc
     2821 acggcggcct tgcccgactg cgccgactcg acgaggcgcg ccatctgggc cagttgagtg
     2881 tcgccgccga cgcgggtcgc ccgcacgcgc aggcgccccg aggtgttcac cgtcgccccg
     2941 gtcaccggat cacccacgcc gacctcgacc ggcaccgact ctccggtcag cagcgaggca
     3001 tcgaccgccg aggctccgtc gacgatgacc ccgtcggtgg cgaccttctc gccaggcctg
     3061 acgacgaact cgtcaccgac aagcaactcc cccaagggga ttcgcacttc gacgtcgcca
     3121 cggagcaccg cgacatcctt ggccccgaga tgcagcagcg cgcgcaacgc cgcccccgag
     3181 cgccgcttgg ccttcgcctc ggcataccgg ccggccagca ggaacgtcgt gatcgccgcc
     3241 gcggtctcga agtagaagtg cccgtgcgcc cccatggtca gcagcatcca cagcgaccac
     3301 ccgaacgctg cgaggacacc accggagaca agcgtgtcca tcgtcgtcgc gccgtgtcgc
     3361 aggttgaccc aggcggcccg atggaacggc cacgcacacc acgtgacgac aggcagcgtg
     3421 agcgcgagca ccacccacgg ccatcccggg aacgccagcg ccggcaccat cgagatggca
     3481 atggcgggaa cgctcagacc cagcgcgacc agcagccgct gccgcaggta tgccgcgtgc
     3541 tcgtcgtccg catccggctc cccaccctcg cccggcccgg gttgggagcc cgggatcgac
     3601 ggcggcgccg gggttgccgt gtaaccggcg gactcgacgg cagccaccaa atcggcgacg
     3661 gccaggccgc ccggatgcac gacgaacgcc ttgccggtgg cgtagttgac cgccgccgtg
     3721 accccggtga gtttgccgag cttcttctcg acccgggccg aacacgaggc acaggtcatg
     3781 ccgccgacga ccagttgggt ctgccgcagc tcggaggggg gtgcctgggt gtccggtcct
     3841 gcggtcatct cttcggttgt ccttcgtctc gcgggtggcg cgtccggtgt cggcgcgccc
     3901 accgacggga cgtcagcgac tggcgctcag cggcctgcca gcgcgtagtt gccggcttcg
     3961 tccagcgcgg cggtgacctc ggcgtcggtc agctcatggt cggcgtcgac gtgaaccgtc
     4021 gaggtgcccc cggcgaccag gtcgacggtg acgtcgctga cgcctgcaac cgacttgagt
     4081 tcggcagtga cgtgaccggc gcaatggccg cacgtcatcc cggtcacctg gaagctctgc
     4141 gtggccattc gggtctcctt cacggggttc tgcgagaacc cctccagcat accccccatg
     4201 ggtatgccgt cggtggttca cccggcaacg aacgccgtca gtggttcatt cccggcatcg
     4261 tcccgccgcc aggcatgtcg tgcgttgcgc cccagcccag gacggtggga tcgagcatcc
     4321 cgaagatcat cgcaacgtgg gccaggacga ggaacacccc gacgaagatc gcgtgccact
     4381 tgcgggcatc ccgcgcgtcc gtcccgatgg caccgacctc gacgagcgtg atgccgatga
     4441 gaggcagacc ggccagcaga taggtgagaa cggcgatgac gtcggccgcg ccgcgccagc
     4501 cgccaccgga cgtgagcggg acgacggcgt tcttcaacag gtgcaccagc acgaggacca
     4561 tcaccggtcc tgcgatcagt ccggcccacc ggctgagcga gcgcacccac gtgggcgggt
     4621 cgctgcggaa gagcaacacc aactccgtga tggccaaggt ttcggccagg atcaccggga
     4681 tggccatgaa gaggatgagg ttccagggct ggttgacggc aagcaacccc atgtagtggg
     4741 tcatggacat gccatccatc gcgccggtgc cgacggtgcg atcgggcaac atccacccga
     4801 tgacgccagc gacgacggcg agcacgccga cgggcgcgag aagggcggat cggcgatgag
     4861 tggtggcgga tgtggtgtcg agagtgctca taactgtcag cctctcgaac cctcgtcaag
     4921 accccctgca cgtctgacga agattcgatc aaggtccgcc ccaccccacg cctccccacc
     4981 acggagatag cgctcgtatg gccacctttc cgtcaattca agcctccaac acccccacac
     5041 atgaccatgc gagcgctatc tccgtggtgg ggggtgggct gggggcacgc ggaggtatgc
     5101 cgcgcggcag ccccagacgc aggtatgccg cgcgtccccc tggacgcgcg gcatacctcc
     5161 tcagctcaac tcggcctcat gctcaagccg gcgtacggct cgaacccgac cggtcagctc
     5221 accttggggg cgcctggcct ggcgtaccgg acccaggtga tcccgatgca cagcaccgcg
     5281 aaggcagcgc cgatccagta gaaggcggtc ggcgacatga gggtcaggcc gacgccgaag
     5341 aggaacggcc cgaacgccgc aatggcgccg gtccagccga tgacgccacc ggcttggcgc
     5401 cgctcgaaga tcatcggcat ctgcttgaac gttgacgcat tgccgatgcc cgcgaacagg
     5461 aagatggcga gcatgcccca aaggaagcgg ccaaagccgg cctgcagcgc ctccgcagaa
     5521 gacgtgtcgg gcaccagccc cccgatcgtg tagatgatcg agcccaccag accgatgccg
     5581 gagacgaggg tccagcgggc cccgcccatc cggtcggtca gcggcgagaa gaggatccgc
     5641 gcgccggcgc cgacgagcgg gccgaggaag acgtacttca cggcgtcggg cacggcatac
     5701 ccggcgacca gcagcgtggc cgtcgagccc gagccggaca cgatggccgc attgcccgcc
     5761 ccatagaggt tggtcatgag caggccgaac tgcgccgaca accccgagaa cgtcccgaag
     5821 gtcatgatgt aaagcagcgt catccaccag gtgtccgggg tgcggaagat gtcgaactgc
     5881 tggcggatgt tggccttgat cggcaccgac ttgagcatcg tccaggcgat gatcgtgccg
     5941 acgatgatga aggggatgta gaagaacgcg gcgttctggt accacacggt cacgtcgggc
     6001 ttgccgggca ccttcatctg ctgggacgcg ccgaggaacc ccaccatccc gaagccgatg
     6061 agccacggcg tcatcaactg cacgagcgag acaccgaagt tgccgatgcc cgcctgcaac
     6121 ccgagcgccg tcccctgcag gcgccgcggg aagaagtagg aggtcgaggg catgaagccc
     6181 gagaacgccc cgccgccgat gcccgcgagg aaggcgagca cgagcagctc ccagtagggc
     6241 gcggtcgggc tctgcacccg cacgccccag ccgaggaccg ggaagaagag cagcgcggtc
     6301 gtcagcgcga ccatcctgcg ggtgcccatg atcggcggga ggatcatcca gacgatgcgc
     6361 atgaggccac cggacaggcc cgggatggcg gccagccagt agagctgttc cttgctgaag
     6421 gtgtagccca acgcgttgag cttgggcacg atcgcgctcg gcaggaacca cgcaacgaac
     6481 gccatggtga gggtgaaggt cgtgatccac agggtcttcc aggcgagcgc cttgtcccag
     6541 gtggcttcgt tctctgggtc ccaagcgtca agccactccc ccgagcgagg ggtggtcggg
     6601 gtgctgctca tgagcggttc tccagttcag ttcgcagata acgagtttcg cgggggtatg
     6661 ccgtccgcgt gacacctcgc agcacaggca tcagccgcgg gtgctcacgt gctcggtgtc
     6721 gaagtggtcg gcgagctcgg gtgagccgct gtgcaacatg tgcacgacgg tccagtgcat
     6781 ccacaccgcg cagatggcgg tgacgatgaa caggcaggcg aaggtgctcg acgggaagcc
     6841 ggtccacgcc ttggtgtagg cgaacatcgg cgggaggaag aacccgccga gtccgccaag
     6901 catgcccacc agaccgccga ccgagccgac gttgtcgggg aagtactcgg ggatgtgctt
     6961 gtagaccgcg gccttgccga tccccatggc cactccgagg aagaacacga ggatcgcgaa
     7021 ggtcggcagc ccgatctggt agcccaggtg ggtggtctgg ctgccgtcgg ggtggctgac
     7081 gacgatgtgg ccgttcggca tcatgaggat tcctgtggtc accagcatga cgccgaaggt
     7141 ccaatacatc acccggcgag cgccgacccg gtcggacacc cagccaccga ggggcctcag
     7201 gagcgaggcc gggaagatga acgtcgcggt gagcagggcg gccggccag
//
LOCUS       DAOMZY010000167         7239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_9679_length_7239_cov_5.579343, whole genome shotgun sequence.
ACCESSION   DAOMZY010000167 DAOMZY010000000
VERSION     DAOMZY010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9679_length_7239_cov_5.579343"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(258..1061)
                     /locus_tag="PLK64_10140"
     CDS             complement(258..1061)
                     /locus_tag="PLK64_10140"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPV80150.1"
                     /translation="MELTRRGALRVGVRAVGAIVLAGCTAAPTPSPGTLGSEGALTST
                     HWPGASPRWRLVRPDPAVVPSPRLVVALHGKGGSVGDASFLGLFDQVSRLGVAVAAVD
                     CGGTYFHARRDGSDVGAMVIDDLLPVLRTQGVSPAPVGLLGWSMGGYGALWLAARYGR
                     DVIGAVGCMSSALWTSPGASAPGAFDDRADFVAHDVFARTKAFAGMPLRLDCGTSDPF
                     IAANRAFAKLVPDAVVTFDAGGHNDTYWRAHGIRQLEWFAQNLPAVSPT"
     gene            1394..1858
                     /locus_tag="PLK64_10145"
     CDS             1394..1858
                     /locus_tag="PLK64_10145"
                     /inference="COORDINATES: protein motif:HMM:NF024788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HPV80151.1"
                     /translation="MRLLRFLPAVVELALLVYCLVDCIQTERSRVRTLPKLGWILLII
                     ILPIIGGVGWLLAGRPRRDGTGDHGAFGTAIRGLGGGPDSSAPRRPLAPEDDPNFMRG
                     LDHGNPEKERLLEEWEAERARREAQEPVAPKDAEDAPGETPGSQERPTDAPG"
     gene            complement(1863..2702)
                     /locus_tag="PLK64_10150"
     CDS             complement(1863..2702)
                     /locus_tag="PLK64_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HPV80152.1"
                     /translation="MSHSPSSDEPAEFAVDSHPDAPPDGDRWSTWDGATHGPGPRPSW
                     VVTDLGAVDADLGVLKTGKEADVHVVRRWVPGDPTHDVFLAAKRFRNSKHRMFHRDAG
                     YLEGRRVRRSRENRAMARRTDFGKEMISGRWAFAEFETLGALWTAGLPVPYPVQFDGT
                     EILMEFIGADGLAAPRLADSRPPETALAGLFDQVREAMLSLAEMGWAHGDLSPYNVLL
                     DVDRIVLIDWPQIVDVIGNPHGFEFLERDARTMAAWFTRRGYAVDPDEFFAQLLGAAT
                     SRW"
     gene            2916..6227
                     /locus_tag="PLK64_10155"
     CDS             2916..6227
                     /locus_tag="PLK64_10155"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HPV80153.1"
                     /translation="MPDKSLGRGGLRVSFPSTHTVGVPVPRKDRDLGAVDTHAILLEA
                     LRDNDFEKVDEFTLTPEVRRDRDPLPRDPGKLTMELDVEGEQDAVVLVEKDGCYSWHL
                     PTGDKARRRGLPGEKRAVRFDIDLAVSDAAYANRRVERGLDDGPRTRGLFGDLLAGAV
                     RVVVMKFAAPLLVGAAVGFLERHVEPGIVHITAPDPATWQRVERLDQLGLPTDRPVRI
                     LLFIHGTFSSTLSAFGSMAVSDNGARFLESALRQYDAVIGFDHPTLSVDPMVNATDLL
                     TRVSSVTNGVSFDVITHSRGGLTTRSFAEYVLPGSGWNGRVEKAVFVAATNNGTHLAD
                     TDRWYDLIDIATNLTMVGAGVLAAMPGGAPVAAVVAGVVKGLGALVKYLGAYAVEEDG
                     VPGLAAMRPGGPFVTGINQTQPGQPTPGTPWFVAKSNFHVTVGDDSHRPPEFPKELAV
                     RLAEGLVDQVFRGDNDLVVDCESMGSIDAAVGGGFVADTYDFESNDVVYHGNYFLQPD
                     FVRRMTDWLLWRVEAAMAEPPEAAAEPPEEAAEPPEVVRGIDFGEPPPPAAPPPPVRG
                     VDVPADPGAPPAAPNVAEATLLASMTAKPSVGVPTPLRVALSRKELHVAAGEVGAVGG
                     FVAPADRPLAVNVAASANARVGLAPDQLGEFATDSFQLPPGGGTSELEFFVEAQAAGP
                     VTVTVVVQDGVKWLKSLALTATAGAPVAPVAAAVFAAPGPLQPEVLASASTPLPASAD
                     QPDFEDYFTIQVQEARFGADTKFLYVVSCPRLGISERFESLPLRARSDYIAATLADVK
                     RAWDDHGAGAAYLETLQDIGADLFGTLFPEDLRALLWANRSKLAPMVLRADEPDVPWE
                     LIHLKPANGTRQKAPAFLGQLGMLRWPFDGYPQATLRARPGRVRVLCPDRAGPAAPGA
                     GVRKEADFLEQRLGASSLPGTEAALRTLLRGGDFDLLHFSGHGVADPKDIGSAKLLLT
                     QVLEGGGAVKLRYLTARAVEQNARLRRDDGSGPLVVLNACQLGRGGDQLTGSGGFAKA
                     FLSTGAAAYVAALWSIHDTPAHDFVTSFYDRLLAGDTVAESAAAAREKARAAGDATWL
                     SYVIYARPDARLVTQ"
     gene            6305..7147
                     /locus_tag="PLK64_10160"
     CDS             6305..7147
                     /locus_tag="PLK64_10160"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019484034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPV80154.1"
                     /translation="MTNRALCVGINQFSNLPMASWLNGCVNDANDMAAMLRKRGFAPR
                     SVTVLTDDQATKANVMAALTKLVETAKEGDHIVFSFSSHGTQVPDTDGDDGRPETDGA
                     DEAFACYDMAQKGDQWDPDTVIIDDELRVLFGKLPKGVVMEAYLDTCHSGDGLRAEDL
                     LAGRRPRFVPPPTLKGLRVIEAVATKSTTTELVKAIPAGGRPVLYAGCRSDQTSADAT
                     FDGRSSGAFTYFFLKALAANPAGTRAEIHKALTKGLVDASFSQRPTLEGPPKAKKAGV
                     GQAW"
BASE COUNT         1128 a         2522 c         2477 g         1112 t
ORIGIN      
        1 cctgcccccg cgccccaccg agatagcgct cgcacggtca tctttcggct gcccaccccg
       61 ggtttgggcc tggagatagc gctcgcatgg tcatgtttcc ggcgcccacc ccgggtttgg
      121 gcccggagat agcgctcgca tggtcatgtt tccggcgccc ggccaggttt gcgcccggag
      181 atagcgctcg cacggtcatc tttcgggtgc ctggccgggt ttgggcccgg agatagcgct
      241 cgcacggtca tgtttcctca ggtgggggag accgccggga ggttttgggc gaaccactcg
      301 agctggcgta tgccgtgtgc tcgccaatag gtgtcgttgt gaccgcctgc atcgaaggtg
      361 acgaccgcgt cagggaccag cttggcgaag gcgcggttgg ccgcgatgaa cgggtcggag
      421 gtgccgcagt ccaggcgcaa cggcataccg gcaaatgctt tagtgcgggc gaagacgtcg
      481 tgggcgacga agtccgcccg gtcgtcgaag gcccccggcg cgctcgctcc gggggaggtc
      541 cacaacgccg agctcatgca gcccacagcg ccgatcacgt cacgtccata gcgcgcggcg
      601 agccagaggg cgccgtagcc gcccatcgac cagcccaaca agcccaccgg agcgggtgag
      661 acaccctggg tccgcaggac gggcaggagg tcatcgatga ccatggcccc cacgtcggag
      721 ccgtcccggc gggcgtgaaa gtaggtcccg ccgcagtcga cggcagccac cgccaccccg
      781 aggcgggaga cctggtcgaa caacccgagg aacgaggcgt ccccgacaga accgcccttc
      841 ccgtggagcg cgacgaccag ccgcgggctc gggacgacgg ccggatcggg ccgcacgagt
      901 cgccatcggg ggctcgcgcc cggccagtgg gtggaggtga gggcgccctc ggagccgagc
      961 gtccccggtg aaggcgtcgg cgcggcagtg catccggcga ggacgatcgc gccgacggcg
     1021 cgcaccccca cgcgcagcgc ccccctgcgg gtcaactcca tgctccgagg gtatgccgcc
     1081 ccctggccac gtgggtccta tcgactgccg cgccccgcgc tgtgcccgtc ggtcatatcg
     1141 acgccccgaa agatcgtcga ggttcgcggt gctcgacgat ctttgctcga cctgacgaag
     1201 gatcgtcgac ctttcaatgg cgccgaggac ggctcgggag gcggtcgcac cgacggcgcg
     1261 cacccccacg cgcagcgccc cctgcgcgtc acctccatgc cccggtgggt atgccgcccc
     1321 gtctggcaca ggtgggtcac acaggtggtg actctcgggt tactctggca gaaccaacga
     1381 gccctttcgg gaggtgcgtt tgctcaggtt cctgcccgcc gtcgtcgagc ttgcgctgct
     1441 cgtctactgc ctcgtcgact gcatccagac cgaacgatcc cgagttcgga cgctgcccaa
     1501 gctcggatgg atcctgctca tcatcattct gcccatcatc ggcggagtcg gctggctcct
     1561 ggccgggcgt cctcgacggg atgggacggg cgaccacggg gctttcggga cggcgatccg
     1621 cgggctgggt ggcggtcctg acagcagcgc ccccaggcgg ccgctggctc cggaggacga
     1681 cccgaacttc atgcgcgggc tcgatcacgg caacccggag aaggaacgcc tcctcgagga
     1741 gtgggaagcc gagcgcgcgc gccgcgaggc ccaggagcct gtggcaccga aagacgccga
     1801 ggacgcacct ggggagaccc ccgggtccca agagcgcccc actgacgccc ccggctgacc
     1861 gctcaccagc gggaggtggc cgcccctaag agctgcgcga agaactcgtc ggggtcgacg
     1921 gcatacccgc gccgggtgaa ccacgcggcc atggtgcggg catcgcgctc gaggaattcg
     1981 aagccgtgcg ggttgccgat gacgtcgacg atctgcggcc agtcgatgag gacgatccgg
     2041 tcgacgtcga gcaggacgtt gtagggggag aggtcgccat gcgcccaccc catctccgcc
     2101 aggctcagca tcgcctcgcg gacctggtcg aacagcccgg cgagggccgt ctccggcggc
     2161 cggctgtcgg ccagccgcgg tgcagccagg ccgtcggctc cgatgaactc catgaggatc
     2221 tcggtgccgt cgaactgaac ggggtaaggg acggggaggc ctgcggtcca caacgcgccg
     2281 agcgtctcga actccgcgaa ggcccaacgc cccgagatca tctccttgcc aaagtcggtg
     2341 cgccgggcca tcgcccggtt ctcccgggag cgacgcacgc gccgaccctc cagatagccg
     2401 gcgtcgcgat ggaacatccg atgcttgctg ttgcggaagc gcttggccgc gaggaagacg
     2461 tcgtgggtcg ggtcgccagg cacccagcgc cgaacgacgt ggacgtcggc ctccttgccg
     2521 gtcttgagca caccgaggtc ggcgtcgacg gcacccaggt cggtgacgac ccacgagggt
     2581 cgggggccag gcccgtgggt agctccgtcc cacgttgacc agcggtcacc gtcggggggc
     2641 gcgtcggggt gcgagtcgac ggcgaactcc gctggctcat ctgacgatgg cgaatgtgac
     2701 acgtgatgac tccgtatgcc gggtggctga ctcccagcct cgcggcatac ccccttggtg
     2761 ggcaacggat tatccgccgg gggtgagagc aggagtcagg tgcggtgtgc gccgaacttc
     2821 cgtgggaccg gatgtatcca gatccctggt caagcactcc tctcacgcga gaaggtgggt
     2881 cctgtcgatc tctccctcga cacaaaggaa ctggcgtgcc tgacaagagc cttggccgcg
     2941 gcggacttcg cgtgagcttt ccgagcaccc acaccgtcgg agttccggtg cccaggaagg
     3001 accgtgacct cggcgccgtc gacacccacg cgatcctcct ggaagcgttg cgggacaacg
     3061 atttcgagaa ggtcgacgag ttcacgctga cccctgaggt gcggcgcgat cgggacccgc
     3121 tgccgcgtga ccccggcaaa ctcaccatgg agctcgacgt cgagggcgag caggacgccg
     3181 tcgtcctggt cgagaaggac gggtgctatt cgtggcatct gccgacgggc gacaaggctc
     3241 gacggcgcgg gctgccgggg gagaagcggg cggtccgttt cgacatcgat ctggccgtga
     3301 gcgacgcggc atacgccaac cgacgcgtcg agcgcgggct cgacgacgga ccacgaaccc
     3361 gtggtctgtt cggcgacctc ctcgccggtg cggtccgggt cgtcgtcatg aagttcgccg
     3421 cgccgttgct ggtcggtgcg gccgtcggct tcctggagcg acacgtcgag ccggggatcg
     3481 tccacatcac tgcaccggat cccgccacct ggcagcgcgt cgagcgtctg gatcagctgg
     3541 gtctgccgac cgatcggccg gtgcggatcc tgctgttcat ccacggcacc ttctcctcga
     3601 cgctcagcgc gttcgggtca atggcggtgt cggacaacgg tgctcgattc cttgagtcgg
     3661 cgcttaggca atacgacgcg gtgatcggct tcgatcaccc caccctgtcg gtcgacccga
     3721 tggtcaacgc gacggacctg ctcacccggg tgtcgtcggt gaccaacggg gtcagcttcg
     3781 acgtcatcac tcacagccgc ggcgggctga cgacgcgcag tttcgcggag tacgtcctgc
     3841 ccggctcggg ctggaacggg cgggtcgaga aggcggtctt cgtcgccgcg acgaacaatg
     3901 gcactcacct ggccgacacc gaccggtggt acgacctcat tgatatcgca acgaacctca
     3961 ccatggtggg tgcgggtgtg ctcgcggcca tgccgggtgg cgctccggtc gcggctgtgg
     4021 ttgccggagt cgtcaaaggt ctcggcgcgc tggtcaaata cctcggggcg tatgccgtgg
     4081 aggaggacgg agtgccgggg ttggcggcga tgcgccccgg agggccgttc gtcactggga
     4141 tcaaccagac ccagcccggg caaccgaccc cgggaacgcc gtggttcgtc gccaagagca
     4201 acttccacgt caccgtcggt gacgacagcc atcgaccgcc ggaattcccc aaggagttgg
     4261 ccgtccggct cgccgagggg ctggtggatc aggtgttccg gggcgacaac gacctggtcg
     4321 tcgactgcga gtcgatgggg tcgatcgacg cagccgtcgg cggcgggttc gtcgcggaca
     4381 cctacgactt cgagtcgaac gacgtggtct accacggcaa ctacttcctg cagcccgact
     4441 tcgtccggcg gatgaccgac tggctgttgt ggcgagtgga ggcagcgatg gccgagccac
     4501 ctgaggcggc ggctgaaccg cccgaggagg cagccgaacc gccagaggtg gttcggggca
     4561 tcgacttcgg cgagccgcca ccaccggcgg cgcctccacc gccggtgcgt ggcgttgacg
     4621 tgcctgctga ccctggggcg cccccggccg ctcccaacgt cgcggaagcc accctcctcg
     4681 cgtcgatgac ggccaagccc tcggtcggag tgccgacccc ccttcgagtg gcgttgtccc
     4741 gcaaggaact ccacgtcgcc gctggtgagg tcggcgcagt tgggggtttc gtcgctcccg
     4801 ccgaccgccc gctggcggtc aatgtcgccg cctcggccaa cgcccgcgtc ggcctggccc
     4861 ccgaccaact cggtgaattc gcgacggata gcttccaact ccccccagga ggtggcacct
     4921 ccgagttgga gttcttcgtc gaagcccaag cggctggccc ggtcaccgtg accgtggtcg
     4981 tccaggacgg cgtcaaatgg ctcaagtctc tggccctcac ggcaacggct ggcgcgccgg
     5041 tcgcgccggt cgcggcggcg gttttcgccg ctcccggccc gctccaaccc gaggtgctcg
     5101 cgtcggcatc gacgccgctg ccggccagcg cggaccagcc cgacttcgag gactacttca
     5161 ccatccaggt ccaggaagcc cggttcggag cggacacgaa gttcctctac gtcgtcagct
     5221 gcccccgact cgggatcagc gagcggttcg agtccctgcc gctgcgcgcc cggagcgact
     5281 acatcgcggc cactctcgcc gacgtcaaac gggcgtggga cgaccacggt gcgggggcgg
     5341 catacctgga gacgctgcag gacatcggag ccgacttgtt cggcacgttg ttccccgagg
     5401 acctgcgcgc gctgctgtgg gccaaccggt ccaaactggc ccccatggtc ctgcgggcgg
     5461 acgagcccga tgtcccgtgg gagttgatcc atctcaaacc ggccaacggc actcgccaga
     5521 aggctccggc gttcctcggt caactgggca tgctgcgctg gcccttcgac ggctacccgc
     5581 aggcgaccct ccgggctcgg cccgggcggg tccgtgtcct gtgcccggac agggcgggtc
     5641 cggctgcacc gggcgccggt gtccggaagg aggccgactt cctcgagcaa cgcctcggcg
     5701 cgtcctcgct gcccggcacg gaagcggcgc tgcggaccct gttgcgtggc ggcgacttcg
     5761 acctgctgca cttctccggc cacggagtcg ccgaccccaa ggacatcggc tcggccaaac
     5821 tcctcctcac ccaggtgctc gaaggcgggg gcgcggtcaa gctgcgctac ctcacggcgc
     5881 gggcggtcga acagaacgcc cggctgcgtc gcgacgacgg gtcggggcct ctcgtggtgc
     5941 tcaacgcgtg ccaattgggc cgtggcggtg accaactcac gggttcgggg ggtttcgcca
     6001 aggccttcct gtccaccggg gcggcggcct acgtcgcggc tctctggtcc atccacgaca
     6061 ctcccgctca cgacttcgtc acgtcgttct atgaccgact gctcgcgggc gacacggtgg
     6121 ctgagtcagc ggccgcagcc cgcgagaagg cccgcgcggc tggggacgcc acctggctct
     6181 cctacgtcat ctacgcccgc ccggacgccc gactcgtcac gcagtgagcg tcaaccaccc
     6241 gcaccacccg tcctgtctct cccaccccac tcctcccgtc cagccagaga aagaggtccc
     6301 aaccatgacc aaccgtgcgc tctgcgtcgg catcaaccag ttctccaacc tgccgatggc
     6361 cagctggctc aacggctgcg tcaacgacgc caacgacatg gcggcgatgt tgcgcaagcg
     6421 cggttttgcg ccgcgatcgg tcacggtgct caccgatgat caggccacca aggccaacgt
     6481 gatggccgcg ttgaccaagc tcgtcgagac ggccaaggag ggcgaccaca tcgtcttcag
     6541 cttcagcagc cacgggaccc aggtccccga cacggacggc gacgacggtc ggcccgagac
     6601 cgacggcgcg gacgaggcgt tcgcctgcta cgacatggcg cagaagggtg accagtggga
     6661 ccccgacacc gtcatcatcg acgacgagtt gcgggtgctc ttcggcaagc tccccaaggg
     6721 cgtggtcatg gaggcctatc tcgacacctg ccactccggc gacggcctgc gtgctgagga
     6781 cctgctcgcc ggtcgccgcc cgcgcttcgt cccgccgccg accctcaagg ggctgcgggt
     6841 gatcgaggct gtggccacca agagcacgac gaccgagttg gtcaaggcga tcccggccgg
     6901 cggccgaccg gtgctctatg ccgggtgccg ctccgaccag acctcggccg acgcgacctt
     6961 cgacgggcgt tccagcggag ccttcacgta cttcttcctc aaggccctgg ccgccaaccc
     7021 tgccggcacc cgggcggaga tccacaaggc cctcaccaag ggtctggtgg acgcgtcgtt
     7081 cagccagcgg cccactctcg aaggcccccc gaaggccaag aaggccggcg tcggccaggc
     7141 ttggtgacga gttccgtcgc ctggtagggc ggcatacccc tggggatccg actagggtca
     7201 ttcgggtatg ccgcccacgg tcgaaggagc ccgcatgag
//
LOCUS       DAOMZY010000168         7227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_9696_length_7227_cov_5.276631, whole genome shotgun sequence.
ACCESSION   DAOMZY010000168 DAOMZY010000000
VERSION     DAOMZY010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7227
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9696_length_7227_cov_5.276631"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PLK64_10165"
     CDS             <1..544
                     /locus_tag="PLK64_10165"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPV80155.1"
                     /translation="LLERPYGVLSGGQRRRVDIARSLLNTPRVLFMDEPTTGLDPQTR
                     RHIWETITRLQHEHDMTIFLTTHYMEEASGSDYVVIVDEGTVAAQGSPAQMKERYAVD
                     QLSVLPKDDDGFSAVVAGLGLPAGSVRRDGPLHILTVTSTAQALRMLEVLGAHIGRFE
                     VTHGSMDDVFLSVTGKALRE"
     gene            544..1407
                     /locus_tag="PLK64_10170"
     CDS             544..1407
                     /locus_tag="PLK64_10170"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV80156.1"
                     /translation="MLGLAARNWKLFYRDRGVVFFSLLGPLIIIGLYILFLKTTVVPE
                     LPVTGSAAEELLNNWIMAGIIASATVTTCLSGYGTMIRDQQSGISKDFTTAPISRSAI
                     IGSYLLNSIVIGTIMSLLTVVVAELYVLAYGGHVMSALQLLKVLGIVVVSVVSAAGVL
                     AVVASLIRTEGAFSGANVAIGAAIGFVVGAYIPIGSLPTEVASVLKTLPSTHSAMLLR
                     QVMLEGPENLAFAGAPASVRSEFDQTMGVVFMVNGSQVTPLQSAAYLVVSGIVGFALA
                     VLIMRARRKGG"
     gene            1461..2120
                     /locus_tag="PLK64_10175"
     CDS             1461..2120
                     /locus_tag="PLK64_10175"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HPV80157.1"
                     /translation="MNVTDVAQRHTAALISVVSPERAERAARYHFVADRMLSLVAGAL
                     ITGCVGRAVGPGVALTFTRGPHGKPQCPQLAALGMDFNVSHTRGWVVCAVGRGSIGVD
                     VEWTREVAPADLAAYLHPDELTDPALSDLLTLWTVREAYVKQLGRGMSQPADTFRVVS
                     TGAGLRILAQGAPADELTVLTARHRSARLAVVSTEPVTVIKQSVSDVVAHASVRSDRS
                     A"
     gene            2120..>7227
                     /locus_tag="PLK64_10180"
     CDS             2120..>7227
                     /locus_tag="PLK64_10180"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HPV80158.1"
                     /translation="MSGIPDVAVRFLDQARATPHLVAVHHNGHELTYAQLAEQVDDLT
                     SRLRAAGVDGGPTVVLLPHGIHLVVAILATFASGSTFVPATDQWPDDYLAAVVRRAGA
                     AVVVTDAPGAVRLAALNGTRAPVAAAVRVVAIDDSHRPESPAGEPRAPGGQERAYLYF
                     TSGSTGTPKAVVGSLRGLGHFIGWEIDEFGVGPGRRTSLLTIPTFDPFLRDIFVPLCS
                     GATLCIPPSAETILDGPALLAWLEDDRIDLVHLVPTLLRELVRGGRPAAAYRPRLLLL
                     AGEPVRGSDITQARDALGDTAELVNLYGPTETTLATCWRRLTLDDATSHIVPVGRPLP
                     GSQVQVCDADGRPQPPGVEGELVIVTHHASHGYLDDPVLTDRSFEGFGGPGPVRYRTG
                     DRGYLTPDGDVVCLGRLDSQVKVRGMKVDLANVERRLRGLPAIRDAVVHQSDDGTLVA
                     AVSAPGGVTAEEIDTALVKVLPRHMVPGRYLVLPGFPTLPNGKVDRQRLRAADQVPDA
                     VSDAVSDAVSDAVTRGVVHLWREVLGAAEVHAGSNFFLLGGHSMLAARLARAIRERFA
                     VTLGAADILQHPTVAAQVALIRAASPAPGIPAADVPIGSEVSPQERAIFLADALATVP
                     TTYLMPWAYRVTGPLDVARIRSVTALLVQRHDALRTTYAFADAGIIARVRPPDAPVDV
                     VRVLDVSDNPPHDEATLLRLLDELTVPFDLSSEIPLRVVVVRLAPDDHVLLFHLHHIA
                     GDEASQVILEQEFALGYVGGDLPPAVGSYRGYAARAAYPRDAADASGRVHQHLTNLRE
                     VPRLNLPRDPSASGRQSNTGRRTRWTLPLELVEDLLRRGAEWGVTPFEITLTGFALAL
                     GGFAETSDLVLGVAAQGREDAHLENTVGVFMSAVPVRCTVDPDGSLHEATMQVAGVAR
                     AARRLGAIPADVLESPQFRRNPGLHPVFDVMVVHQDPDRPELALPGCAIEPLRMAGTT
                     SRYDLTLYLHPRSSGWDAEFEYCTDLFSEGYVRALWHALCTVLAEICHRPDLALREVE
                     LVSVGAEPLPGGIDAALSPLPPALVHERFAAHAQMTPDAPALCFGDEEFSYAQVRDRA
                     AAIAVALRRHPAFRPGAPVGVMMSRTPDLVATLLAVLASGAPYVPLDVGFPPARIAHI
                     LDRSRVGLIATDGTVVAVTPDVPSLDVTTVRATHSVEEPVAHGPVDVALTDSAYQIFT
                     SGSTGFPKGVDISHGNLVNFVDGIAEAVGLDRDLRVCCLTTVSFDIFVLEVLVPLALG
                     GVVVLADEREQLDLAALAKLLRRHRVNTLQLTPSRLSAFLDAADAEAHLQGISTFVIG
                     GEPITQDGVRRLLSFAGPPRVFNAYGPTETCVWSTVKRIESVDDLTIGRAIKNTRLYV
                     MDRRLRLRPRGLPGELCIGGHGVATGYVHDPHRTTRAFVADRYHPGERIYRTGDIVRW
                     NDRDELAFIGREDQQVKVRGYRVELREIEIALNAHPDIREAVCVLTTGHTGAAGELHA
                     AYTSAEPIPDGVLRSHLAEHLPSYCVPTGFWRLAELPVTPNAKIDRNAVKAWLTTSLG
                     SATRPDGQPNGNGVFGTGAQATDPESAFVRMTWAELLGRSTFDDDANFFDVGGNSLLL
                     VRLHNAIERRYPGRLTLPDLFGYPTVGLTATQLRTSAASAAATATASATATAVATAAA
                     PTPLPVVVLRNEFRVRSGNGTRAVDHPSPVDPTLG"
BASE COUNT         1052 a         2557 c         2413 g         1205 t
ORIGIN      
        1 cctcctcgaa cgtccctatg gcgtcctctc ggggggccag cggcggcgcg tggacatcgc
       61 ccgctcgctg ctcaacaccc cccgggtcct gttcatggat gaacccacga cggggctgga
      121 ccctcagacg cggcggcaca tttgggagac catcacgcga ctgcagcacg agcacgacat
      181 gaccatcttc ctcaccactc actacatgga ggaagcgtcc ggctccgact acgtcgtcat
      241 cgtcgacgag ggcaccgtcg cggcgcaggg ctcgccggcg cagatgaagg aacggtatgc
      301 cgtcgaccaa ctgagcgtgc ttcccaagga cgatgacgga ttcagcgcgg tcgtcgccgg
      361 actcgggctc ccagcgggga gtgttcgccg ggacggcccc ctgcacattc tgacggtgac
      421 gtccacggca caggccctga gaatgctcga ggtcctcggc gcgcatatcg gacgattcga
      481 agtcacgcac ggaagcatgg acgacgtgtt tctgtcggtc accgggaagg cgttgcgcga
      541 ataatgctgg ggctggcggc gaggaactgg aaactgttct accgggatcg gggcgtcgtc
      601 ttcttctcct tgctcggtcc gttgatcatc atcggcctct atatcctctt cctcaagacg
      661 accgttgttc ccgaactgcc cgtgacgggg tcggccgccg aagaattgct caacaactgg
      721 atcatggccg gcatcattgc ctcagcgacc gtgaccacct gcctgtcggg ctacggcacc
      781 atgatccgcg accagcagag cggcatttcc aaggacttca cgacggcacc cattagccgc
      841 agtgcgatta tcggctccta tctgctgaac agcatcgtca tcgggacgat catgagcctg
      901 ctcaccgtgg tggtggccga gctgtatgtc ctggcctatg gcgggcatgt gatgtcggcc
      961 ctccaactcc tcaaggttct cggaatcgtc gtggtgtcag tcgtgtccgc ggcgggtgtg
     1021 ctggccgtcg tcgcgagcct cattcgcacc gagggtgcgt tcagtggggc gaacgtcgcg
     1081 atcggtgccg ccatcgggtt cgtcgtcggg gcctatatcc cgatcggaag cctcccgacg
     1141 gaggttgcgt cggtgctcaa gacgctgccg tccacgcact cggcgatgct gctgcgccag
     1201 gtgatgttgg aaggacccga gaacctggcc tttgccgggg ctccggcctc ggtgaggagc
     1261 gaattcgacc agacgatggg tgtcgtcttc atggtcaacg gatcgcaggt cacccccctc
     1321 caatccgcgg cctatctcgt cgtgagcggg atcgtcgggt tcgccttggc cgtcctcatc
     1381 atgcgcgctc gcaggaaggg cggctgaccg ccacggcggt ccgccggtcg agggtcatca
     1441 tgtcggggag agtggtggtg gtgaacgtca ccgatgtcgc gcagcggcac accgctgcgc
     1501 tgatatccgt ggtgtcgccc gagcgcgccg agcgagccgc ccgctatcac ttcgttgccg
     1561 accgaatgtt gagtctcgtt gcaggggcgc tcatcaccgg gtgtgttggc cgcgccgtcg
     1621 gccccggcgt cgccctgaca ttcacacgcg gccctcatgg caagccgcag tgcccgcaat
     1681 tggcggccct gggaatggac ttcaacgtct cccacacccg tggatgggtc gtgtgtgcgg
     1741 tgggccgagg ctcgatcggc gtcgacgtcg aatggacgcg cgaggtggca cccgccgacc
     1801 tggcggcata cctgcacccg gacgagttga ccgatcccgc actgtcggac cttctgaccc
     1861 tgtggacggt ccgcgaggcg tatgtgaagc agttggggcg gggcatgtct cagccggccg
     1921 acaccttccg ggtggtctcg accggtgcgg ggctgcggat tctcgcccag ggtgcgccgg
     1981 ccgacgagct cacggtcctg accgcccggc atcgcagcgc ccggctggca gtcgtgtcca
     2041 ccgagccggt cacagtcatc aagcagtccg tgagtgacgt ggtggcgcac gcgtcggtcc
     2101 gatcggacag gtcggcctga tgagcggcat acccgatgta gccgtccgct tcttggacca
     2161 ggcccgggcg accccgcacc tggtcgccgt gcaccacaac ggtcacgagt tgacctatgc
     2221 ccagctcgcc gagcaggtcg acgacctgac cagtcggctg cgcgcggcgg gggtcgacgg
     2281 ggggccgacg gtcgtcctgc tgccccacgg catacatctg gtcgtcgcga tcctggccac
     2341 cttcgccagc ggcagcacct ttgtcccggc aaccgaccag tggcccgacg actatctggc
     2401 cgcggtcgtg cgtcgcgcag gcgcggcggt cgttgttacc gatgcaccgg gagcggtccg
     2461 gctcgctgcg ctgaacggga ccagggcacc ggtcgcggct gctgtccggg tcgtggcgat
     2521 cgacgatagc caccgaccgg agagcccggc aggcgagccg cgcgcgccgg gtggacagga
     2581 gcgggcctat ctctacttca cgtccgggtc gaccgggaca cccaaagccg tcgtcggcag
     2641 tctgcggggc ctggggcact tcatcgggtg ggagatcgat gagttcggcg tcggccctgg
     2701 gcgacgaacc agcctgctga ccatccccac cttcgatccg ttcctgcgcg acatcttcgt
     2761 tccgttgtgc tcgggagcga ccttgtgcat cccgccgtcc gcggagacca tccttgacgg
     2821 accggccctg ctggcctggc tcgaggacga caggatcgac ctggtccacc tggttccaac
     2881 cctgttgcgc gaacttgtcc gcggcgggcg gccagctgcg gcataccggc cccgcctgct
     2941 gctgctcgcc ggtgagccgg tgcgcgggag cgatatcacc caggcgcggg acgccctcgg
     3001 tgacaccgcc gagctcgtca acctctacgg tccgaccgag accacccttg ccacctgctg
     3061 gcggcggctg accctcgacg acgcaacgag ccacatcgtc cccgtcgggc gcccgctccc
     3121 cggcagccag gtgcaggtgt gcgacgccga cggccgaccc cagccgccag gcgtggaggg
     3181 cgagctcgtc atcgtcaccc accatgcctc acacggctac ctcgacgacc ccgtgctcac
     3241 cgaccgatcc ttcgaggggt tcggcggacc gggaccggtg cggtatcgaa cgggggatcg
     3301 cggctatctc accccggacg gcgacgtggt ctgcctgggt cgcctcgatt cccaggtcaa
     3361 ggtccgcggg atgaaggtcg acctcgccaa cgtcgaacgg cggctgcggg ggctaccggc
     3421 gatccgcgac gccgtcgtcc accagtcgga tgacggaacg ctggtggcgg ccgtgtcggc
     3481 cccgggtggg gtgaccgccg aagagatcga cacagcgctc gtgaaggtgc tgccgaggca
     3541 catggtgccc ggtcgctatc tcgtcctgcc cggcttcccc acgctgccca acgggaaggt
     3601 ggaccgccag cggctgcgcg ccgccgacca ggtgccggac gccgtgtccg acgccgtgtc
     3661 cgacgccgtg tccgacgccg tgacgcgtgg tgtggtgcac ctgtggcgcg aggtgctggg
     3721 cgcagcggag gtccatgcgg gaagcaactt cttcctcctc ggtgggcact cgatgctcgc
     3781 ggcccgcctg gcccgggcga tccgcgagcg gttcgcggtc actctcggcg ccgcggacat
     3841 cctccaacat cccaccgtgg cagcccaggt cgccctgatc cgcgccgcgt cacccgcccc
     3901 tgggataccg gccgcagacg tgccgatcgg atcggaggtg tcaccacagg agcgagccat
     3961 cttcttggcc gacgccctcg ccaccgtgcc gacgacctac ctcatgccgt gggcctatcg
     4021 ggtgactggc cctctggacg tcgcccggat ccggagcgtc accgccctcc tcgtccagcg
     4081 gcacgacgca ctacgaacca cgtacgcgtt cgcggacgcc ggcatcattg cgcgggtccg
     4141 ccctcccgac gccccggtgg acgtggtccg ggtcctcgac gtgtccgaca acccgccgca
     4201 cgacgaggcc acgctcctgc ggctgctcga tgaactgacc gtgcccttcg acctctcgag
     4261 cgagatcccg ctgcgcgtgg tcgtggtgcg cctggccccc gacgatcacg tgctgctgtt
     4321 ccacctgcac cacatcgccg gtgacgaggc ctcccaagtg atcctggagc aggagttcgc
     4381 gctcggctac gtcggtggcg acctgccgcc cgcggtgggg agttaccgag ggtatgccgc
     4441 tcgcgcggca tacccccgcg atgccgccga cgcgtcgggg cgggtccacc agcatctgac
     4501 caacctgcgg gaggttccgc ggctgaacct cccgcgcgat ccgtcggcat ccggacggca
     4561 gagcaacacc ggccgacgaa cccggtggac cctgccactc gagctggtcg aggacctgct
     4621 ccgccgcggc gccgagtggg gcgtgacccc cttcgagatc accctgaccg gctttgcgtt
     4681 ggcgctcggc ggcttcgccg agacgagcga cctggtactt ggggtcgcgg cacagggtcg
     4741 ggaggacgcg cacctggaga acaccgtcgg ggtgttcatg tctgcggtcc cggtgcgctg
     4801 caccgtcgac ccggacggga gtctccacga ggcgacgatg caggtagccg gcgtggctcg
     4861 ggccgctcgg cgtctcgggg cgatccccgc cgacgtcctc gaatctccac agttccggcg
     4921 caaccccggt ctgcatccgg tcttcgacgt catggtggtc catcaggacc ccgaccgacc
     4981 cgagttggcc ttgcccgggt gtgccatcga gccgctgcgg atggcgggca cgaccagtcg
     5041 ttacgacctc acgctgtatc tgcacccgcg atcgagcggt tgggacgcgg agttcgaata
     5101 ctgcaccgat ctgttcagcg agggctacgt ccgggccctg tggcacgcgc tgtgcacggt
     5161 cctggccgag atctgtcatc ggcccgacct cgcgctgcgc gaggtggagt tggtgagtgt
     5221 gggtgccgag cccctgcccg gcggcatcga cgcggccctc agtcccctcc cgcccgcgct
     5281 cgtgcacgag cggttcgcgg cacacgccca gatgacgccc gacgcccccg cgctgtgctt
     5341 cggcgacgag gagttcagct acgcacaggt gcgggatcgg gccgccgcca ttgccgtggc
     5401 ccttcgtcgc cacccggcct tccgccctgg ggcgccggtc ggcgtcatga tgtcgcggac
     5461 ccccgacctc gtggcgacgc tcctcgccgt cctggcctcg ggcgcgccct acgtgccact
     5521 ggacgtcggg tttcctcccg cgcggatcgc ccacatcctg gatcgcagtc gagtgggctt
     5581 gatcgccacc gacggaacgg tcgtcgctgt cacgcctgac gtcccgagtc tcgacgtgac
     5641 cacggtgcgg gccactcact ccgtcgagga gcctgtcgcc cacgggccgg tggacgttgc
     5701 cctgaccgac agcgcctatc agatcttcac gtccggatcg acgggcttcc ccaagggcgt
     5761 cgacatcagc cacggcaacc tcgtgaactt cgtcgacggc atcgccgaag cggtgggcct
     5821 cgaccgcgac ctgagggtct gttgcctcac caccgtctcg ttcgacatct ttgtccttga
     5881 agtgctggtc ccgttagccc tcggcggggt ggtggttctt gccgacgagc gcgaacagtt
     5941 ggacctcgcg gcgttggcca agttgcttcg gcggcaccgg gtcaacaccc tccagttgac
     6001 cccttcgcgg ctctcggcct tcctggacgc ggctgacgcc gaagctcacc tgcaggggat
     6061 ctcgactttc gtcatcggtg gcgagccgat cacccaggac ggcgtgcgcc ggctgctctc
     6121 cttcgccggc cccccgcggg tcttcaacgc ctacgggccc accgagacct gcgtctggag
     6181 caccgtcaag cgcatcgagt cggtcgatga cctcaccatc ggccgggcga tcaagaacac
     6241 ccggttgtat gtcatggacc gacgcctgcg actgcgcccc cgcgggctgc cgggtgagct
     6301 gtgcatcggt ggccacggcg tggccacggg ctatgtccac gacccgcacc gcaccactcg
     6361 cgcgttcgtc gccgaccgct accaccccgg ggaacggatc taccgcaccg gggacatcgt
     6421 caggtggaac gaccgcgacg agctcgcgtt catcggtcgg gaggaccagc aggtcaaggt
     6481 ccggggctac cgggtcgagt tgcgggagat cgagatcgcg ctgaacgcgc atccagacat
     6541 ccgcgaggcc gtgtgcgtgc tgacgacggg ccacaccggt gcggccgggg aactgcacgc
     6601 ggcatacacg tcggccgagc cgatccccga cggcgtgctg cgcagccacc tcgccgagca
     6661 cctgcccagc tactgcgtgc ccaccggctt ctggcgcctg gcggagttgc cggtcacccc
     6721 caacgcgaag atcgaccgga acgccgtcaa ggcgtggctc acgacgtcac tcggctcggc
     6781 cacgcgcccc gacggccagc ccaacggcaa cggtgtgttc ggcaccgggg cccaggcgac
     6841 cgaccccgag tccgccttcg tgcgaatgac ctgggcagag ctgctgggtc gctcgacgtt
     6901 cgatgacgat gccaacttct ttgatgtcgg cgggaattcg ctccttctcg tgcggctgca
     6961 caacgcgatc gagcggcgct accccgggcg gctgaccctg cccgacctct tcggctaccc
     7021 gaccgtgggg ctcaccgcga cccagctacg aacgtccgcg gcgagcgcag cggcgactgc
     7081 gacggcgagc gcgactgcaa cggcagtcgc aacggcggcg gcacccactc cgctcccggt
     7141 ggtggtgttg cgcaacgagt tccgcgtgcg cagtggcaat ggcactcgcg ccgtcgacca
     7201 tccgtctccc gtggacccga cgctggg
//
LOCUS       DAOMZY010000169         7219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_9708_length_7219_cov_5.614740, whole genome shotgun sequence.
ACCESSION   DAOMZY010000169 DAOMZY010000000
VERSION     DAOMZY010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7219
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9708_length_7219_cov_5.614740"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..1115
                     /locus_tag="PLK64_10185"
     CDS             237..1115
                     /locus_tag="PLK64_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HPV80159.1"
                     /translation="MSDFETIAWTVDADGIATLTLNRPDRLNSFTVTMARELEHAFRH
                     EALDDDVRAVVVTGSGRAFCAGMDLSAEGNVFGLDESLLPTPEHFWEHYADEPLHDGV
                     RDTGGRVTLAIHDLPKPVIAAVNGPAIGIGTTMTLAMDVRLASTTARFGLVFGKLGIV
                     PEAASTWFLPRIVGLAQALEWMYAAPVLTADAALAGRLVRSVHEAEDLLPAAYELARS
                     FVVGKSPVALALTRKLLYRNVGVEHPLQAHLADSLGMYYTSIGDGREGVAAFLEKRAP
                     HFNGRASDLPRITPEQ"
     gene            complement(1759..3063)
                     /locus_tag="PLK64_10190"
     CDS             complement(1759..3063)
                     /locus_tag="PLK64_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HPV80160.1"
                     /translation="MDQVTDPESGTARHTSAPGGETGDGGPSRCPAVHFDHTSAEHSL
                     DPVASYEALRAQGPVVWSPEHGGYWVLTDYASVFAAARDDETFSSARSAHGGEGLNNV
                     IPKAPVHLHIPVELDPPEHRTYRKIINAVTAPAALDGLRPRISYWTARFVDDVIEAGQ
                     ADLATVIGVPAAVTLDWLGMDASQWQQYSHTMHSVLANRPGTDAHAHALAEGIPWMSQ
                     QITDVIAARRAQPRDDIVSYLVGAQVDGAPMSDDAAYSIIELLISGGVGTTASLVSQT
                     LVHLDADRSQRAELIEHPERLERAIEEFLRAFSPTQALARTIVRDTEIAGCPMRAGDR
                     ALLAWSSANRDPQQFEAPDEVRIDRWPNRHTAFGLGVHRCAGAHIARAIAAELLGQIL
                     TRMPDYVVDRDRLRAYPQQGVNAGYEHIPATFTPGARLLPAE"
     gene            3207..4244
                     /locus_tag="PLK64_10195"
     CDS             3207..4244
                     /locus_tag="PLK64_10195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HPV80161.1"
                     /translation="MEIGVILGDVHGSLSPRDHLDALLRQVEAAQRAGMTYLTIGHHY
                     VYGDYRWLQPIPTLARLAAELDPGVRLGTTVLQTPLVHPIALAEDLATLDILSRGNLV
                     IGLGAGYREFEFVALGVNFADRFAMLEESIELMTALWTQARVTHRGRFWQVDDVEPHI
                     QPWQQPHPPLWIGAMTERGVKRSARLADGWPITPETKIPDMVRLLAIYEDERIRLGKP
                     LVRHPLRREIIPGRTTEAAFERFESMAKERLLAYARRQLATRSEDELSQEFRSVAAKE
                     TFIGTPDECIAQIRELAALVPIDPILVRAQWPRMSADQVVDYLEDLGRDIVPAVREIT
                     PVQQVIRPPVA"
     gene            4329..4694
                     /locus_tag="PLK64_10200"
     CDS             4329..4694
                     /locus_tag="PLK64_10200"
                     /inference="COORDINATES: protein motif:HMM:NF022279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2568 domain-containing protein"
                     /protein_id="HPV80162.1"
                     /translation="MPLAVGVLGALAFATELLLWAGVAVAVQAAVGGWLGWVLGLLVA
                     VLVIVAWSVWMAPRASHRIAPVLRAIVAGFGCLGVGSALLSQGPHWWGIALPMGAVVM
                     VVAELKPDRGREPSDSGGR"
     gene            complement(4696..5850)
                     /locus_tag="PLK64_10205"
     CDS             complement(4696..5850)
                     /locus_tag="PLK64_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HPV80163.1"
                     /translation="MTGPLHGVRVVELAGIGPGPFAAMLLADMGADVVRVDRVDPPSR
                     DYTPNAVIERGRRSVAVDLRRPAGAAIVRRLASGADVLVESNRPGVLERLGLGPDVLL
                     ADNPRLVIGRLTGYGQDGPLAGAVGHDLNYISLTGALHTIGRAGEPPPPPLNLVGDFG
                     GAGSALAFGVVCALWEARGSGAGQVVDANALESTAALMGLVHGLRAAGRWSLDRGANL
                     LDGGTPYYDTYTCADGRWVAVATVEEKFFLELLRGLGLDDDPDLVGAHKDRSRWPALR
                     AALMAVFASRTRDEWAVHFAPLDACVTPVLDLDEAAGHPHAVARGGYVATEGTAYPQP
                     AVSPRFSRSSTPLPGAPARPGEHTRAVLTEAGYATAQVDELLATGVARQA"
     gene            complement(5847..>7219)
                     /locus_tag="PLK64_10210"
     CDS             complement(5847..>7219)
                     /locus_tag="PLK64_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPV80164.1"
                     /translation="LARRGVRAGDPVALVMPNCPDVVLVWAALLQLGAVAVCADPRSS
                     AAELGYVLEHSRAVGAVVDPVVTARAREPLDKLPWVVESSGRPEVGRGTGLLMADPPA
                     GWATVPAQAPASIQYTSGTTSRPKAVVWTQANCLWAGRVGAAHQGLTQADTNLVHLPL
                     FHTNALSYSLLSTLFAGGHVVLQPRFSASRFWPVAVEHGATWSALVSFCVRALQTREP
                     LPGHRFRGWGNSAALDPAPWSGGVAALGWFGMTETVTHPIIGSLDPRDPPGTMGRPAR
                     EYAVAVLHPDGSPVGAGEVGDLRVRGIPGVSLFAEYLRDPEATAAAVDADGWLRTGDR
                     VRLNEGGTLSFVEREGDLLKVGGENVGAAEIERVLLQAPGVAEVAVVGAPHEMLGEVP
                     VAFVLPRAGASVIVAEAEARCVAGLSPLRRPREIRIVSELPRVTLDKVAKAVLRGILR
                     EEGVRR"
BASE COUNT         1073 a         2636 c         2361 g         1149 t
ORIGIN      
        1 acatggtgac cgggtcgatc caccgctgag gtcggcgggc gaggtcccag aggtggacat
       61 gcgcgtcgat caccgtcgct gccacgctcg cctcctgtga tgcgatccca catctaacca
      121 tatgatatgc atagataatg tcttcccccc taccgattcg aggtatttcg ccatcgtctg
      181 gtgatggcgg aatacctcga cccggcagag gggccagcca cgaaagcgag atgccgatgt
      241 cagacttcga gaccatcgcc tggaccgtcg atgcggacgg catcgccacc ctcaccctca
      301 accggcccga ccggctcaac tccttcaccg tgacgatggc tcgcgagctt gagcacgcct
      361 tccgccacga agcgctcgat gacgacgtcc gcgcggttgt cgtcaccggc agcggccggg
      421 cgttctgcgc gggtatggac ctctcggctg agggcaacgt cttcggtctc gacgagtcgc
      481 tgcttccgac ccccgagcac ttctgggagc actacgccga cgagccgctg catgacggcg
      541 tgcgcgacac cggcggccgg gtcaccctcg cgatccacga cctgcccaag ccggtcatcg
      601 ccgcggtcaa tggcccagcg atcggcatcg gcacgacgat gaccctcgcg atggacgtcc
      661 ggctcgcctc gacgacggcg cggttcggct tggtcttcgg caaacttggc atcgtccccg
      721 aggcagcgtc cacctggttc ctcccgcgga tcgtcgggct cgcgcaggcc ctcgagtgga
      781 tgtatgccgc gcccgtcctc accgccgacg cggccctcgc ggggcggctg gttcgctcgg
      841 tgcacgaggc cgaggacctg ttgcccgcgg catacgagtt ggcgcggtcg ttcgtcgtcg
      901 ggaagtctcc ggtcgcgctc gccttgaccc ggaaactgct ctaccgcaac gtcggcgttg
      961 agcacccgct gcaggcccac ctggccgact cgctcgggat gtattacacg tcgatcggcg
     1021 acgggcgcga gggcgttgcc gcgttcctgg agaagcgggc gccgcacttc aacggccgcg
     1081 cctcggacct ccctcggatc accccggagc aatgatcagg accggcgctt gacgcgctgg
     1141 ttccggatgc tgcctcgcag atgcaggggc gactgctggc ggcgaagcct cgcttgtgtt
     1201 gagttatcac gactaccctt agacagttag ggcagtcccc tgaaggattc ctccccattt
     1261 ctttcggacc tgaatgcacg cagcccgcca gaggacatca tgaacctgac ctcctaccag
     1321 aagacgcgag tgggtctggt tgcctggggt gcggctggcc tgttgctcac tctgggcgcg
     1381 gtgtccctgg ggcatgtcgc ctgggtcatt tccatggcgt tcctgctgat ctccgcggtc
     1441 gtccgggccc ctggtcgtcc gtccgccctg ttctttgggg cttccgttcc actggttctg
     1501 gttgtcgtgg gtcacgccgc gggccccggt tggtcgtgcc actccagtcc cacaggttct
     1561 gggtgcgatg agttgctcga ccctcgaccc tacttgatgg cggcgttggt gctgcttgcc
     1621 accggcactg cactcgaact tctgttgcgg cgagcgagtt cgcgtggcac ggcgatccac
     1681 agttcttgac gattcctcgt cacctctgat cgttaaggcc actgccgcag aacgcaattc
     1741 gtgcctgagg cggcgctctc actccgctgg cagcagccgc gctcccggcg tgaacgtcgc
     1801 cgggatgtgc tcgtagcccg cgttgacgcc ctgctgcgga taggcgcgca gccgatcgcg
     1861 gtcgacgacg taatcgggca tccgcgtgag gatctgcccc aacaactcgg ccgcgatggc
     1921 ccgcgcgata tgcgctccgg cgcagcgatg gacccccagc ccgaaggccg tatgccggtt
     1981 gggccaacgg tcgatccgca cctcgtcggg cgcctcgaac tgctgcggat cacggttggc
     2041 cgagctccac gcgagcagcg cccgatcgcc ggcccgcatc gggcaaccgg caatctcggt
     2101 gtctcgcaca atggttcgtg ccaacgcctg cgtcggtgag aacgcccgca ggaactcctc
     2161 gatcgcccgc tccagccgct ccgggtgctc gatgagctct gcgcgctgcg accgatcggc
     2221 gtcaaggtgc acgagtgtct ggctcaccag cgaggccgtc gtccccacgc ctcccgagat
     2281 gagcaattcg atgatgctgt atgccgcgtc gtcgctcatc ggggcaccat ccacctgcgc
     2341 gcccacgagg taggacacga tgtcgtccct cggctgcgcc cgcctggccg cgatcacgtc
     2401 ggtgatctgc tggctcatcc acgggatgcc ctcagcgagc gcgtgcgcat gcgcgtccgt
     2461 gcccggccgg ttggcgagca ccgagtgcat ggtgtgcgaa tactgttgcc attggctggc
     2521 atccatgccg agccagtcga gcgtcacggc cgccgggacc ccgatgacag tcgcgagatc
     2581 cgcctgccca gcttcgatga cgtcgtcgac gaaccgcgct gtccaatagg agatccgcgg
     2641 acgcagcccg tcgagcgccg ccggcgcggt gaccgcgttg atgatcttgc gatacgtgcg
     2701 gtgctccggt gggtcgagct ccaccgggat gtgcaggtgc accggcgcct tggggatgac
     2761 gttgttcagc ccctcgccgc cgtgcgcgga ccgcgccgac gagaaggtct cgtcatcccg
     2821 cgcggccgcg aacaccgacg catagtcggt caacacccag tagccgccgt gctccgggct
     2881 ccacaccacc ggcccctgcg cccgtagcgc ctcataggac gccactgggt ccagcgagtg
     2941 ctcggcgctt gtgtggtcga aatgcaccgc gggacagcga ctcggcccac catcgccggt
     3001 ctcgccgcca ggcgcgctgg tatgccgcgc agtcccggac tcggggtcgg tcacctgatc
     3061 catggggcac cttccacgtc gtcggcgttg actggctctg acccttgctg cgggcgctgg
     3121 cccggctctc ggacgccagc cctcccgcat cgcggattcc atgcatatga tagccatcat
     3181 ctggtgatcg cggacggagc tggcccatgg agatcggcgt catcctcggc gacgtgcacg
     3241 gctcgctttc gccgcgggac caccttgacg cgctgctgcg ccaggtcgaa gccgcccagc
     3301 gggccgggat gacctacctg accatcggcc accactacgt ctacggcgac taccgctggc
     3361 tgcaacccat tccgacgctc gcccggctgg ccgccgaatt ggaccctggg gttcggctcg
     3421 ggacgaccgt cctgcagacc ccgctcgtcc acccgatcgc cctcgccgaa gacctggcga
     3481 cgctcgacat cctcagccgg ggcaatctcg tcatcggact cggtgccggc taccgggagt
     3541 tcgagtttgt cgctctcgga gtcaatttcg ccgatcgctt tgccatgctg gaagagtcga
     3601 tcgagctgat gaccgccctg tggacccagg ctcgggtcac ccaccggggc cgcttctggc
     3661 aggtcgacga cgtcgagccg cacatccagc cctggcaaca gccgcaccca ccgttgtgga
     3721 tcggcgcgat gactgagcgg ggcgtcaagc gctcggcgcg cctcgccgac gggtggccca
     3781 tcacccccga gaccaagatc cccgacatgg tgcggctcct cgcgatctac gaggacgagc
     3841 ggatccgcct cggcaagccc ctcgttcgcc acccgttgcg ccgcgagatc atccccggcc
     3901 gcacgaccga ggcggccttc gagcggttcg agtcgatggc caaggagcgc ctgctcgcct
     3961 atgcccggcg ccaactcgcc acccgctcgg aggacgagct ctcccaggag ttccgctctg
     4021 tcgcagccaa agagaccttc atcggcacgc ccgacgaatg catcgcccag atccgtgagc
     4081 tcgccgccct cgtccccatc gacccgatcc tcgtgcgcgc ccaatggccc cggatgagcg
     4141 ccgaccaggt cgtcgactac ctcgaggacc tcggccggga catcgtcccc gccgtccgcg
     4201 agatcacgcc ggtccagcag gtcatccgcc caccggtcgc ctagggtcgc gaatgtgagc
     4261 gggtatgtcg tcctgtctgc agtggtccgc gccggggtgg ccgtgccggc cgaatcttcg
     4321 ggggcctgat gccgctcgcg gtcggcgtgc tcggggcgct cgcgtttgcg acggagctgc
     4381 tgctgtgggc gggtgtggcc gtggcggtcc aggctgcggt cgggggttgg ctgggttggg
     4441 tccttggtct gttggtggcc gtcctggtca tcgtcgcctg gtcggtgtgg atggcccctc
     4501 gagccagcca tcggatcgct cccgtactcc gcgcgatcgt ggccggattc ggctgtcttg
     4561 gcgtcgggtc agcactgctc tcgcaaggcc cgcactggtg ggggatcgct ctccccatgg
     4621 gtgccgtcgt catggtggtc gccgaactca aacctgaccg cgggcgtgag ccttccgact
     4681 cgggcggccg ctagctcacg cctgtcgggc caccccggtg gcgagcaact catcgacctg
     4741 cgcggttgca tacccggcct cggtgaggac cgcgcgggtg tgctcaccgg gacgggcggg
     4801 cgcgcccggg aggggggtcg agctccgcga gaagcgtggg gacaccgccg gctgcgggta
     4861 tgccgtgccc tccgtcgcga cgtagcctcc ccgcgcgacc gcatggggat gcccggcggc
     4921 ctcgtcgaga tccagcacgg gggtcacgca cgcatccagc ggggcgaagt gcaccgccca
     4981 ctcgtctcgg gtccgactgg cgaacaccgc catcaacgcc gcccgcaggg ctggccaccg
     5041 tgaccgatcc ttgtgcgcgc caacgagatc cgggtcatcg tcaagcccca accccctcag
     5101 caactcaagg aagaacttct cctcgaccgt cgcgaccgcc acccaacggc catccgcgca
     5161 ggtgtaggtg tcgtaatagg gcgtcccccc gtccaggagg ttggccccgc gatccaggct
     5221 ccagcgaccg gccgcgcgca gcccgtgtac caaccccatc aaggccgctg tcgactccag
     5281 cgcattggca tcgacgacct gcccggcccc cgaccctcgg gcctcccaca gtgcgcagac
     5341 gaccccgaaa gccagcgccg acccagcccc accaaagtcg ccgaccaggt tgagcggcgg
     5401 gggcgggggt tccccggccc ggccgatggt gtgcagcgcc cccgtgagcg agatgtagtt
     5461 gaggtcgtgg ccgaccgctc cggcgagcgg tccgtcctgg ccataaccgg tgagccgccc
     5521 gatgaccagt cgagggttgt cggcgagcaa cacgtccggc cccagcccca gccgctccag
     5581 caccccaggc cggttgctct cgacgagcac atctgcgcca gaggccaatc ggcgcacgat
     5641 cgccgcgccc gcgggtcggc gcaggtcgac ggccaccgag cggcgacccc gttcgatcac
     5701 ggcgttgggt gtgtagtccc gcgatggcgg gtcgactcga tcgacccgca cgacatcggc
     5761 gcccatgtcg gccagcagca tcgccgcgaa cggccccggc ccgatcccgg cgagttcgac
     5821 gacgcgcact ccgtgcaacg gcccagtcat cggcgcaccc cctcctcgcg cagtatgccg
     5881 cgcagcaccg cctttgcgac cttgtcgagc gtcacgcgtg gcaactcgct gacgatgcgg
     5941 atctcgcgtg gtcgtcgcaa ggggctcagc ccggcgacac aacgggcctc cgcctcggcg
     6001 acgatgacgc tcgccccggc gcggggcagc acgaacgcca ccggcacctc gccgagcatc
     6061 tcgtgcggtg ccccgacgac cgccacctca gcgacgcccg gcgcctgcaa cagcacccgc
     6121 tcgatctccg ccgcgccgac gttttcgccg ccgaccttga gcagatcgcc ctcccgctca
     6181 acgaaactca gcgtcccgcc ctcgttcaac cgcacccgat caccggtccg caaccagccg
     6241 tcggcatcga ccgccgcagc ggtcgcctcc ggatcgcgca gatactccgc gaacaacgac
     6301 acgccaggta tgccgcgcac ccgcaggtcg ccgacctcgc ccgcccccac gggggagccg
     6361 tcggggtgca ggacggcgac ggcatactcc cgcgcggggc gacccatcgt gcccggcggg
     6421 tcgcgcgggt ccagcgagcc gatgatcgga tgggtcaccg tctcggtcat cccgaaccag
     6481 ccgagcgccg ccaccccgcc cgaccatggc gccgggtcga gcgcagcgct gttgccccaa
     6541 ccgcggaacc ggtggccagg caacggttct cgggtctgca gcgcacgcac gcagaaagac
     6601 acgagggccg accaggtcgc cccgtgttcc acggcgaccg gccagaagcg ggaggccgag
     6661 aagcgtggct gaagcacgac gtgaccgccc gcgaagagcg tcgagagcag cgagtaggac
     6721 aacgcgttgg tgtggaagag cggcaggtgc acgaggttgg tgtcggcctg ggtcagcccc
     6781 tgatgggcag cgccgacccg gcccgcccac aggcagttgg cctgggtcca gacgacggcc
     6841 ttggggcgcg aggtcgtccc cgaggtgtat tggatcgagg ccggtgcctg ggccggcaca
     6901 gtcgcccacc ccgccggagg gtcggccatg agcaacccgg ttccccgccc cacctccggg
     6961 cgcccactgc tctccaccac ccagggcagt ttgtcgaggg gctcccgcgc acgtgccgtc
     7021 acaacggggt cgacgacggc tccgacagcg cgcgaatgct ccaagacata gcccaattcg
     7081 gctgcgctgg agcgcgggtc agcgcagacg gccaccgcgc cgagctgcag cagcgcggcc
     7141 cagaccagca cgacatcggg gcagttgggc atgacgaggg ccaccgggtc gccggctcgg
     7201 acaccccggc gagccagcc
//
LOCUS       DAOMZY010000170         7176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_9767_length_7176_cov_9.263446, whole genome shotgun sequence.
ACCESSION   DAOMZY010000170 DAOMZY010000000
VERSION     DAOMZY010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7176
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9767_length_7176_cov_9.263446"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /locus_tag="PLK64_10215"
     CDS             <1..1290
                     /locus_tag="PLK64_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80165.1"
                     /translation="GNAKAMKELGIKFKDTGDTGKNFDQVVGGLKDTVGGFAEKEAGT
                     LDGKLGILKTKFGDVQESVGEKLVPALTKLIDVAVKVVDWMGKNSDILIPLAAGLGIV
                     AVAFAAVNAVMALNPFVLVAAAVGLLVAGLIYAYRESETFRKVVDTAFTAIGKAGKWL
                     WNEALQPALKWIVNGFAWVTEGLADMLGALGKVPGFGWAKDAADDLRGVAEKARDAAE
                     GIKDIPDADPKIEARDKATAVVALIDKQIKSLRGKVVEAKAKGDDKGVRDLQAKIKAL
                     QGKRVAIVATLSASGSASWRITGGAGTGSLRAIPAARGGIFRAMAAGGINEARIYSQA
                     DGVQFNEPDTQGEGYIPLANDWRRPRAVEVWRETGRRIGAMAGGGINGSPLGGSGMTV
                     NVNATVQRGVDPLAFAREVEQELTKLARTRGGRLAFS"
     gene            1287..1532
                     /locus_tag="PLK64_10220"
     CDS             1287..1532
                     /locus_tag="PLK64_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80166.1"
                     /translation="MTYTPYHADWRDRPDPSTPITAAAIEHIEQGIVDAHNLIAGLGN
                     ALQVAPGVVARLVGVGLTSNPDPAMLQAGDFWIMDVS"
     gene            1529..2968
                     /locus_tag="PLK64_10225"
     CDS             1529..2968
                     /locus_tag="PLK64_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80167.1"
                     /translation="MIRHVELWATLDGVRSKWLHGGTKPEASIPATVTAAWSDANPPW
                     AAGTTEDVITAEWPPGVRIDTIATSGGTFQERLDTANAGDGLRVIRVGSGVHHLSGFR
                     LSGTSGSVNFAHGYYHGNMRGWLGTGAASSIIQQDSSMTAPQLAYLSSMTAAAFAPNK
                     MMMALLQPVGEGATVYCGGVTFRAVDQPALTSVAADLVSKGIYTPQPAPHGGIEVGQG
                     KRAVFSYCRFVGTSHAMYAAPPFEHGGLTSQYSPSILIDHCEVDGRRATEVDPARPWR
                     GGLVMGNNETLWEARDSWFHHCNLSRIAVNDENRATFGTYRFVRCKIDHVGNRNVDPA
                     LNGGATLGGSTNAVVAGFESVAGLVEFIDCDLSVDNPNTSGSVSQHIGFSSTGGRNPQ
                     GGRLRVVGGTFRNTAFPSIDGFLTIRVIPTTYWALDGYATTIDVRAVAGGPRKSPWVY
                     TGTWPPVASQIAAAGVTPETHYIVRPKTT"
     gene            2978..3997
                     /locus_tag="PLK64_10230"
     CDS             2978..3997
                     /locus_tag="PLK64_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80168.1"
                     /translation="MADYTFDSDAEGASASGLPQQTGLTAYAASAFHGARGVRSAGSG
                     TEGRARFAPTGTSVGVREYFRFAGATPGVIAALMWFHAAGTVRVGSVEIQSDSRIRFS
                     DASGLAPAKWTSTTTIAADTWYRLEVTLTCGATTSTGSVKLGLYLGDSTTALETYTAT
                     DLNTGAGLNLLYAYVGKFGTAAATPDFDSMGYVEPATDLIGRYTPGAASLVRPLSVLS
                     NTGGISVGGTGASTLTQALSTSDASYLVNPDSASNESVTFRLPPLTVGTSVIETPRWG
                     LWPGSASVPMRVEVLQGAAVVCSDDFTVTSETPASVSVTLSAPEVAAITDWTDLSMRV
                     TWKVA"
     gene            4001..4291
                     /locus_tag="PLK64_10235"
     CDS             4001..4291
                     /locus_tag="PLK64_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80169.1"
                     /translation="MAQIVKLYDLTVGTDQFQRVRLYDLTVGRAETGIHKVKLYDLTV
                     TAPSVHKVKLYDLTTTTDVQQATLIRYWDGSAIRYGYLAWWDGSAIVSALSE"
     gene            4301..6085
                     /locus_tag="PLK64_10240"
     CDS             4301..6085
                     /locus_tag="PLK64_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80170.1"
                     /translation="MAYPGDVLGPITYPGEDGAGSGAEDYSVIIGGHEAKDYLLGTIT
                     LTRGRQGEGAIQPSILTFAVDNSDARFTPGGTGAWSADVMRGATVTAYASGVRRFTGT
                     VATVALSWPHGVDLIASATITCADVSANWSEPIESLYHEWIKACHPLLWWPLTESNAP
                     ALEIAQGDYANRLVPRVIGEGTVDMLNWGGATGHPADEAASLLVDNLLTVSGAFLESV
                     SALDYDPSIPNVVVTLVVNTRDVDLPVLTLEDGGFVSQAVQGVSRFDIYRGTTEDAAG
                     NIGYGWAPASPPSSTGVLMMPMRTPDVIGCKVRVGAGHKLTDWDTTVVTRDGSFAHLA
                     ILPAVAPFSTYPLPYRPPTTAAGPVSDKIEYLTGLVLGDPAEVVVSGAEPVVAHLPQS
                     GASIADVLSRWSEGTGSRWVIDPSGRVSWVASNGGERVTISAEWLSPSSAPVIDGQGV
                     ANTVDLTLPSGQVWTYTTGAQPIVRRAMVGAFGDTARSKALAQWVANMPDGVRLPTAE
                     FDLLTIPLDQAMVVAALNIGDRLSITDLPTQMPDPTWTGVVEGATETWGSGAWSVAYS
                     LTPDFRTAMWTLDHPTLSQLDAGNRLGF"
     gene            6101..6595
                     /locus_tag="PLK64_10245"
     CDS             6101..6595
                     /locus_tag="PLK64_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80171.1"
                     /translation="MARRPVANEAVTSGGLTDMINYRSAVLNASSLATIAGSKTATVN
                     VGYLAIPATDYASVIDVDGQFWCELGATADWSVRFGYDTAADPGATGTGTPGATLQRD
                     YLHNPFGTPVKRTANVSGKFVLAANTAAWVRMLAVRTAGTLTIGTPIGLVLNAHRTEI
                     FPAA"
     gene            6592..7017
                     /locus_tag="PLK64_10250"
     CDS             6592..7017
                     /locus_tag="PLK64_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80172.1"
                     /translation="MTGPVARFGRRGVALILIGVAWVGFGLTLLAAPPVPDADAAIFM
                     DYIPAVWQFAAWAFTGMVAVVAGARRPWQWVGYIAATIMPILRATSFAWSTIMWLVPG
                     APEGDSRSVGAGLMWTALIGLVLVVGSWPDTTHLPEAAP"
BASE COUNT         1109 a         2309 c         2514 g         1244 t
ORIGIN      
        1 ggcaacgcga aggccatgaa agagcttggg atcaagttca aggacaccgg ggataccggc
       61 aagaacttcg atcaggtcgt gggcggtctg aaggacaccg ttggcgggtt cgcggagaag
      121 gaagcaggga cgctcgacgg caagctgggc atcctcaaga ccaagttcgg cgacgtgcag
      181 gagtcggtgg gcgagaagct cgtgcccgcc ctcacgaagc tgatcgacgt ggccgtgaag
      241 gtcgtggact ggatgggcaa gaactccgac atcctgattc cgctggccgc tggtctgggg
      301 attgtggccg tggcgttcgc tgccgtgaac gccgtcatgg cgctgaaccc gttcgtcctg
      361 gtggctgctg ccgtgggtct gctggtcgct ggcctgatct atgcctatcg cgagtcggag
      421 acgttccgca aggtggttga cacggcgttt acggcgatcg ggaaggccgg caagtggctg
      481 tggaatgagg cgctgcaacc ggcgctgaag tggatcgtga acgggttcgc gtgggtcact
      541 gaagggctcg ccgacatgct gggtgcgctc ggcaaggtgc ccgggttcgg gtgggcgaag
      601 gatgccgccg acgacctgcg gggcgtggcc gagaaggcac gcgacgccgc cgagggtatc
      661 aaggacattc ccgacgctga cccgaagatc gaggcccgcg acaaggcgac ggctgttgtt
      721 gccctgatcg acaagcagat caagtctctg cgcggcaagg tggtggaggc gaaggcgaag
      781 ggtgacgaca agggcgtccg ggacttgcag gccaagatca aggcgctgca ggggaagcgg
      841 gtagccattg tcgcgaccct atcggcgtcg ggttcggcgt cgtggcggat cactggcggg
      901 gccggtaccg gctcgctgag ggccattcct gcggcccgtg gtggcatctt ccgggccatg
      961 gccgccggtg gcatcaacga ggcgcgcatc tactcccaag ctgacggcgt tcagttcaac
     1021 gaaccggaca ctcagggcga gggttacatc ccgctcgcca acgactggcg tcggccccgg
     1081 gctgtggagg tctggcgcga gaccggccgg cgcatcggtg ccatggctgg gggcggcatc
     1141 aacggttccc cgctgggcgg atcgggcatg accgtcaacg tcaacgcgac cgtccagcgt
     1201 ggcgtcgacc cgttggcgtt cgcccgtgag gttgaacagg aactcaccaa gttggcacgt
     1261 acccgaggcg gaaggctggc attctcatga cctacacccc ctatcacgcc gactggcggg
     1321 accgtcccga cccgtcgacc ccgatcaccg ccgccgcaat cgagcacatc gagcagggca
     1381 tcgtcgacgc acacaacctg atcgccggat tgggcaacgc gttgcaggtg gcgcccggtg
     1441 tggtggcccg gctggttggg gtcggtctga cctcgaaccc tgacccggcc atgctacagg
     1501 cgggcgactt ctggattatg gacgtgtcgt gatccgtcac gtcgaactgt gggccacctt
     1561 ggatggtgtg cggtcgaagt ggctgcacgg cggcacgaaa cctgaggcgt cgatccctgc
     1621 cacggtgacc gcggcatggt cggacgccaa tccgccgtgg gcggctggga ccaccgagga
     1681 tgtcatcacg gccgagtggc cgcctggtgt gcggatcgac acgatcgcca cgtcgggggg
     1741 cacattccag gagcgccttg acactgcgaa cgccggcgac gggttgcggg tgatccgggt
     1801 cgggtcgggt gtgcaccacc tgtccgggtt ccggctgtcg ggcacgtcgg gcagtgtgaa
     1861 ctttgcccac ggctactacc acggcaacat gcgcggctgg ttgggaacgg gcgcggcgtc
     1921 cagcatcatc cagcaagatt cgtcgatgac ggcgccccag ttggcctacc tgtcttcgat
     1981 gacggccgcg gcgttcgcac cgaacaagat gatgatggcc ctcctgcagc cggtgggcga
     2041 gggggcgacg gtctactgcg ggggcgtcac gttccgggcg gtggatcagc ccgctctcac
     2101 gtcggtggcc gccgacctcg tgtcgaaagg catctacacc ccccagcctg cccctcatgg
     2161 cggcattgag gtggggcagg gcaagcgggc cgtgttctcg tactgccggt tcgtggggac
     2221 gtctcacgcg atgtacgcag ccccgccgtt cgaacatggc gggctcacgt cgcagtattc
     2281 gccgtcgatc ctgattgacc attgcgaggt cgacgggcgc cgcgcgaccg aggttgaccc
     2341 ggcccggccg tggcgtggcg gtttggtgat gggcaacaac gagaccttgt gggaggctcg
     2401 ggattcgtgg ttccatcact gcaacctgtc ccggatcgcc gtcaacgacg agaaccgggc
     2461 cacgttcggg acgtaccggt ttgtgcgctg caagatcgac catgttggga accgcaacgt
     2521 tgatccggcc ttgaacggtg gggccacgtt gggtggttcc acgaacgcgg ttgtggccgg
     2581 gttcgagtcg gtcgctgggc tggtcgagtt catcgactgc gacctgtcgg tggacaaccc
     2641 gaacacgtcc ggctcggtgt cgcagcacat cggcttctcg tccaccggtg gccgtaaccc
     2701 gcagggtggg cggctgcgtg tggtgggcgg cacgttccgc aacacggctt tcccgtccat
     2761 cgacgggttc ctgaccattc gcgtgatccc caccacctat tgggctctgg acgggtacgc
     2821 gaccacgatc gacgtgcgcg cggtcgctgg tggcccgcgc aagtcgccgt gggtgtacac
     2881 gggcacatgg ccccctgtgg cgtcgcagat cgccgccgcc ggcgtcaccc ctgagacgca
     2941 ctacatcgtc cggccgaaga ccacctagga gacccctgtg gctgattaca cgttcgactc
     3001 tgacgccgag ggcgcttccg cttccggctt gccccagcag acgggcctca ccgcctatgc
     3061 cgcgtcagcg ttccacggcg cccggggcgt ccggtcggct gggtccggca cggaaggccg
     3121 ggcacggttc gccccgacgg ggaccagcgt cggtgtccgg gaatacttcc ggttcgctgg
     3181 ggcaaccccc ggggtcatcg ccgctctcat gtggttccac gcggcgggca cagtccgggt
     3241 cgggagcgtc gagattcagt ccgactcgag gatcaggttt tcggatgcgt ccggcttggc
     3301 gcctgcgaag tggacgtcaa cgaccaccat cgccgctgat acgtggtacc gcctcgaggt
     3361 gaccctcacc tgtggcgcca ccacgtcgac ggggtcggtg aagttgggcc tgtacctggg
     3421 cgactccacg acggcgctgg aaacgtacac ggccaccgac ctgaacacgg gtgcgggcct
     3481 gaacctgctg tacgcctacg tgggcaagtt cggcaccgcc gccgccacgc ccgacttcga
     3541 ctcgatgggg tatgtggagc ccgccacgga cctgatcggc cgttacacgc ctggcgccgc
     3601 gtcgttggtg cgtccgctgt cggtgctgtc gaacacgggc ggaatctcgg tgggcgggac
     3661 gggtgcgtca accctgacgc aggccctgtc cacgtctgat gcgagctacc tcgtgaaccc
     3721 ggattcggcc agcaacgagt cggtcacgtt ccggctgccc ccgttgacgg tgggtaccag
     3781 cgtgatcgag acgccgcgct ggggtctgtg gcccgggtcg gcttcggtgc ccatgcgggt
     3841 cgaggtgttg cagggcgcag cggtcgtgtg ttccgacgac ttcaccgtga ccagcgaaac
     3901 accagcgtcg gtttcggtca ccttgtcggc gcctgaggtt gcggcgatca ccgactggac
     3961 ggacctgtcg atgcgcgtca cgtggaaggt ggcctgaccc atggcgcaaa tcgtcaagct
     4021 gtacgacctg actgtgggga ccgaccagtt ccagcgggtc aggttgtacg acctgacggt
     4081 gggacgcgcc gagacaggca ttcacaaggt gaagctgtac gacctgaccg tcacggctcc
     4141 cagcgtccac aaggtgaagc tgtacgacct caccaccacc accgacgtgc agcaggcgac
     4201 cctgatccgc tactgggatg ggtcggcgat ccgctacggc tatctggcgt ggtgggacgg
     4261 gtctgctatc gtgtccgcgc tgtccgagta ggaggctgcc atggcgtatc ccggtgacgt
     4321 tctggggccc atcacctacc cgggcgagga cggtgcaggg tccggcgctg aggactattc
     4381 cgtcatcatc ggcgggcatg aggcgaagga ctacctgctc ggcaccatca ccctcacccg
     4441 cggcaggcaa ggtgagggcg cgatccagcc gtcgatcctc acgttcgccg tcgacaactc
     4501 cgacgccagg ttcaccccgg gcgggacggg tgcgtggtcg gctgatgtga tgcgcggcgc
     4561 cacggtgacc gcctacgcgt ccggggtccg gcggttcacc ggcaccgtgg cgacggttgc
     4621 gttgtcgtgg ccgcacgggg tcgacctgat cgcgtcggcg acgatcacct gcgcggacgt
     4681 gtccgcgaac tggtccgaac cgatcgaatc cctgtatcac gagtggatca aggcgtgcca
     4741 tccgctgctg tggtggccgc ttaccgagtc gaacgcgccc gcgctggaaa tcgcccaagg
     4801 cgactacgcg aaccggctcg ttccccgcgt gatcggcgag ggcaccgtcg acatgttgaa
     4861 ctgggggggc gccacagggc atcccgccga tgaggccgcg tcgctgctgg tcgacaacct
     4921 gctcaccgtg tcgggggcgt tcctcgagtc agtgtccgca ctcgactacg acccgagcat
     4981 cccgaacgtg gttgtgaccc tcgtcgtcaa cacccgcgac gtggacctgc ccgtgctcac
     5041 cttggaggac ggcgggttcg tgtcgcaggc cgtgcagggc gtgtcccggt tcgacatcta
     5101 ccgcggcacc accgaggacg ccgccggcaa tatcgggtac gggtgggcgc ctgcgtcgcc
     5161 gccgagctcc accggtgtcc tgatgatgcc catgaggacc cctgacgtga tcggctgcaa
     5221 ggttcgggtt ggcgctgggc acaagctgac cgactgggat acgaccgtcg tcaccaggga
     5281 tgggtcattc gcacatctgg cgatcctgcc ggcggtggcc ccgttctcga cctacccgct
     5341 gccgtaccgg ccgcccacta ccgcagccgg gcccgtgtcc gacaagatcg agtacctgac
     5401 cgggctcgtg ttgggtgatc cggccgaggt ggtcgtgtcc ggcgctgagc cggttgttgc
     5461 ccacctgccc cagtcggggg cgtccatcgc tgacgtgctg tcgcgctggt cggagggcac
     5521 cgggtcgcgt tgggtgatcg acccgtccgg ccgcgtgtcg tgggttgcgt cgaacggcgg
     5581 tgaacgggtc accatcagcg ccgaatggtt gtcccccagt tcggcgcccg tcattgacgg
     5641 gcagggtgtc gccaacacgg tcgacctgac gttgcccagc gggcaggtgt ggacctacac
     5701 caccggcgcc cagccgattg tgcggcgcgc catggtgggg gcgttcggcg acacggcgcg
     5761 gtcgaaggcg ttggcgcagt gggtggcgaa catgcccgac ggcgttcggc ttcccactgc
     5821 cgagttcgac ctgctgacca tccccctcga tcaggccatg gtggtggccg cgttgaacat
     5881 cggcgaccgg ctgagcatta ccgacctgcc cacgcagatg cccgacccga catggacggg
     5941 ggttgtggag ggcgccaccg aaacgtgggg ctcgggggcg tggtcggtgg cctacagcct
     6001 caccccggat ttccggacgg cgatgtggac cctcgaccat ccgacattga gccaactcga
     6061 cgccggcaac cggctcggct tctgaaggaa ggaacacaac atggcacgca ggccagtagc
     6121 gaacgaggcc gtcacatcag gcgggctgac cgatatgatc aactaccggt cggcggtcct
     6181 gaatgcgtca tccttggcga ccattgccgg cagcaagacc gcaaccgtga acgtcggcta
     6241 tctggcgatc ccggccaccg actacgcgtc tgtgatcgac gtcgacgggc agttctggtg
     6301 cgaactgggc gccacggctg actggtcggt ccggttcggc tacgacacgg ccgcagaccc
     6361 gggagcgacc ggcaccggga cgccgggggc gacgttgcag cgcgactacc tgcacaaccc
     6421 gttcgggacg ccggtcaagc gcacggcgaa cgtgtcggga aagttcgtcc tcgccgcgaa
     6481 cacggccgca tgggtccgga tgctggccgt ccgcacggct gggacgctca ccatcgggac
     6541 gccgatcggg cttgtgttga acgctcaccg caccgagatt ttcccggccg catgaccggt
     6601 cctgtcgccc gcttcggtcg ccgcggcgtg gccctcatcc tgatcggcgt cgcctgggtc
     6661 gggttcggct tgacgctgct tgcggcgcca ccggtcccgg acgccgacgc cgcgatcttc
     6721 atggactaca tccccgcggt ctggcagttc gccgcgtggg ctttcacggg catggttgcg
     6781 gtcgtcgctg gggcgcggcg tccgtggcag tgggtcggct acatcgccgc caccatcatg
     6841 ccgatcctcc gcgcaacttc gttcgcctgg tcaaccatca tgtggttggt tccgggcgcc
     6901 cctgaggggg attcgcgctc ggtcggcgct ggcctgatgt ggactgccct gatcgggctc
     6961 gtcctggtgg tcggctcatg gccggacact acccacctcc cggaggctgc cccgtgacca
     7021 accctgcaac catcctgtcc accatcctcg gagcggtcgc tgttgtcgtc gtcggatgga
     7081 tgacgggacg cgctacccgc cgcgtcgccg ccattgaggg ggcgacaccc gcctacaccg
     7141 aactggatgc ccgcgtgcaa cggctggagg tgcgcg
//
LOCUS       DAOMZY010000171         7166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_9784_length_7166_cov_5.970750, whole genome shotgun sequence.
ACCESSION   DAOMZY010000171 DAOMZY010000000
VERSION     DAOMZY010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7166
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9784_length_7166_cov_5.970750"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PLK64_10255"
     CDS             complement(<1..304)
                     /locus_tag="PLK64_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80173.1"
                     /translation="MGRHQTDAPPVRPRWVIPLVAFAVAVGGGGGAWWAISRAGGPST
                     EAGPASSASGGTAAAVTTPCTSSVAVQVAVAGPLVPAVRTAADLATASGACATCAVT"
     gene            complement(425..1318)
                     /locus_tag="PLK64_10260"
     CDS             complement(425..1318)
                     /locus_tag="PLK64_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HPV80174.1"
                     /translation="MPHPSPVRLARVHADVLRWYAVHARPLPWRAPGTSPWGVYVSEI
                     MAQQTPVARVEPVWREWLTRWPTPADLAAATPGDAVRAWGRLGYPRRALRLHDAARVM
                     VDRHDGQVPTELTDLLALPGVGAYTAAAVACFAHGIPEAVVDTNVRRVLARAITGTAL
                     AAPTLTAAETALAVAALPADHDDALTWNVAVMELGALVCTARAPRCEVCPIRSECAWQ
                     LAGAPPDAGPARRGQAWVGTDRQARGALLQALRDAPGTVRSMTLEAVWPDPIQRARCL
                     DGLVADGLVEPLAGQRFRLPG"
     gene            complement(1323..2399)
                     /gene="disA"
                     /locus_tag="PLK64_10265"
     CDS             complement(1323..2399)
                     /gene="disA"
                     /locus_tag="PLK64_10265"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HPV80175.1"
                     /translation="MERTDDDLLIATLAAVAPGTELRDGLERILRSGTGALIVIGSDK
                     SVEAICSGGFRIDAEFSATRLRELTKMDGAVVIDKDVAKIIRANTQLVPDSRIETSES
                     GTRHRTAERVAKQTGLPVISVSKSMQTIALYIGGRRHVVENSASILSRANQALQTLER
                     YKGRLDEVTGTLSALEIEDLVTVRDVAAVLQRLEMVRRISEEIHGYAVELGTDGRLLS
                     LQLEELVGGLGNDRELVIRDYLPVGKGAKTVDAAMADLVGLSATELLDLAAGARAVGF
                     AGPADGLDTSVSPRGYRLLTRVPRLPGAIIDRLVEHFDSLQRLLAANIEDLMAVEGVG
                     ESRARAVREGLSRMAESSILERYV"
     gene            complement(2480..3871)
                     /gene="radA"
                     /locus_tag="PLK64_10270"
     CDS             complement(2480..3871)
                     /gene="radA"
                     /locus_tag="PLK64_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPV80176.1"
                     /translation="MATNNSVRARERGTAYRCSECGWSTVKWVGRCGECQAWGTVGEA
                     GDGRPRTTAPARAVPPPARIGEVDRTAAVARPTGVSEFDRVLGGGLVPGAVVLVAGEP
                     GIGKSTLLLDVAARAARSGRDVLYVSGEESAAQVRLRAERIEAMASSLFLAAETDLAT
                     ILGQIEAVTPDLVIVDSVQTIASAQVEGAAGNVAQVREVAASLIHVAKTRGIAVLLVG
                     HVTKDGSIAGPRLLEHLVDVVVQFEGERHSRLRLVRAAKNRYGPTDEVGCFDLSDVGI
                     VGLPDPSGLFLSSREVAVSGTCVTVTLEGRRPLVAEIQGLVTASTLPTPRRATHGLDA
                     SRIAMILAVLQQRAGAQIGGKDAYVATVGGVKVTEPAADLAIALAVASALRDEPFPSD
                     GIAFGEIGLAGEIRSVAGMPRRLAEAARLGFRRAWVPRGVAGSGPMPDGIRIIEVSQV
                     WEAVGRALSSSSR"
     gene            complement(3910..4509)
                     /locus_tag="PLK64_10275"
     CDS             complement(3910..4509)
                     /locus_tag="PLK64_10275"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPV80177.1"
                     /translation="MLALAGRSDWSGATVLDIGCGAGFHLPRLAESAEAVIGVEPHAP
                     LVALAQDRTAGLDNVSVRLASAQATGLPGQSVDVATARWAYFFGPGCEPGLVELTRIM
                     RPGGLAFLVDVDATRSTFGAWFRGSLPDYDPAAVERFWRRQGWVQERLEVAWEVSTRS
                     DFEAVVRLEFAPTYAGRILAEDPDRTGVDYAVNLWWRRF"
     gene            complement(4663..5460)
                     /locus_tag="PLK64_10280"
                     /pseudo
     CDS             complement(4663..5460)
                     /locus_tag="PLK64_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145095.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5516..6460
                     /locus_tag="PLK64_10285"
     CDS             5516..6460
                     /locus_tag="PLK64_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HPV80178.1"
                     /translation="MRLGVIDVGSNTVHFLVVDAYYGGHPLPAYSHKVELKLSENLLP
                     DGTIGAEFGRALTQFLSECQEIAEEQGVIDLMAFATSAIRDAQGGQELIETVSRESGV
                     DLQVLSGRDEARLTYLAVRRWFGWSSGRLLVVDIGGGSLELASGLDEEPDAAISLPLG
                     AGRLTRDLLADDLPKPKDIKAARAYVRAEIGGAVRELLRFGPPTRAVGTSKTIRSLAR
                     IAGAAPSGEGIYVTRRLRREHLGDIVKRLGRMSVAERAELPGVSATRAHQLLAGALVV
                     EGAMDLLRLDRLDIAPWALREGIILRRLDHLEADGPLS"
     gene            6502..>7166
                     /locus_tag="PLK64_10290"
     CDS             6502..>7166
                     /locus_tag="PLK64_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPV80179.1"
                     /translation="MHIRVSLSTASVYPGTCATAFELASRLGYDGIEVMVWTDVVSQE
                     AGALRALSRHYGIPVLSVHAPTLLITQRVWGPEPWTKIDRSIELAADLDAPTVVLHPP
                     FRWQKEYAAEFVDGVAAREAESGVRLAVENMFPWRARGRDLQAYLPHWDPVPMSYDNV
                     TLDLSHTATAGSDALAMAADLGERLTHVHLADGSGSVRDEHLVPGRGGQPCAEFLQLL
                     PGSG"
BASE COUNT         1041 a         2499 c         2615 g         1011 t
ORIGIN      
        1 cggtcacggc acacgtcgcg caggccccgg aggccgtggc gaggtcggcg gcagtgcgca
       61 ccgcggggac cagcggcccc gcaacggcga cctggaccgc gacgctggac gtgcacgggg
      121 tggtcaccgc agcagcggtg cccccgctgg cgctgctcgc tggccccgct tcggtggacg
      181 ggcctccagc gcgggagatc gcccaccacg cgccgcctcc gcccccgaca gccacggcga
      241 acgcgaccag tggaatgacc catcgaggcc ggacgggcgg agcgtcggtc tgatggcgac
      301 ccatgcgtgt gtgtcctccg agctgggaga gtgggcagag gtggggagag tgagcaaccg
      361 cggggctcac gctagttgtc cggagcacct acccgatagt tgcctgccga tccggtgcga
      421 gcggtcagcc tggtaagcgg aaccgttggc cggccaacgg ctcgaccagg ccgtccgcga
      481 cgagtccgtc caggcagcga gcccgttgga tcgggtcggg ccagaccgct tccagcgtca
      541 tcgagcgaac ggtgccgggc gcgtctcgca gcgcctgcag gagagcgccg cgggcctgcc
      601 tgtcggtgcc gacccaggcc tgcccacgcc gcgccgggcc tgcgtcgggc ggtgccccgg
      661 cgagctgcca ggcgcactca gatcggatcg ggcagacctc gcagcgaggg gcccgtgccg
      721 tgcagacaag ggcccccagc tccatgacgg cgacgttcca ggtgagggcg tcgtcgtggt
      781 cggcggggag ggccgcgacg gccagcgcgg tctcggccgc cgtcagggtc ggcgcggcga
      841 gggcggtgcc ggtgatagcg cgggcgagca cgcgccgcac gttggtgtcg acgacagcct
      901 ccggtatgcc gtgtgcgaag caggccacgg cggcggcggt gtaggcgccc acgccgggca
      961 gggcgagcag gtcggtcagc tcggtcggca cctgaccgtc gtgccgatcc accatcaccc
     1021 tggcggcgtc gtgcagtcgc agtgcgcggc gcgggtagcc cagccgcccc caggcccgca
     1081 ccgcatcacc gggtgtcgct gccgcgaggt cggcgggtgt gggccagcgg gtcagccact
     1141 cccgccagac cggctcgacg cgggccaccg gggtctgctg cgccatgatc tcgctgacgt
     1201 agacccccca agggctcgtc ccgggagccc gccagggcag cggtctggcg tggacggcat
     1261 accaacgaag gacgtcggcg tgcacccggg caagccggac gggggagggg tgcggcacgc
     1321 ggtcagacgt agcgctcgag gatgctcgac tcggccatcc gggacaggcc ctcgcggact
     1381 gcacgggccc ggctctcacc gaccccctcg accgccatga ggtcctcgat gttggctgca
     1441 agcaggcgtt ggagcgagtc gaagtgctcg acgagccggt cgatgatcgc tcccggcaga
     1501 cgcggcacgc gcgtgagcag gcgataaccg cgcggcgaga ccgaggtgtc caggccgtca
     1561 gccgggccgg cgaagccgac cgcgcgagcg ccggcggcca gatcgagcag ctcggtggcc
     1621 gacaggccga cgaggtcggc catcgcagcg tcgacggtct tggcgccctt gcccacgggg
     1681 aggtagtcgc ggatcaccaa ctcgcggtcg ttgcccaggc cgccgacgag ctcctccaat
     1741 tgcaggctga gcaggcggcc gtcggtgccg agctccacgg catacccatg gatctcctcg
     1801 ctgatccggc gaaccatttc caggcgctgc aggacagcgg cgacatcgcg gacggtgacg
     1861 agatcctcga tctccagggc cgacagggtg ccggtgacct cgtcgagtcg gcccttgtag
     1921 cgttcgaggg tctgcagggc ctggttggcg cgggagagga tgctcgcgga gttctcgacg
     1981 acgtgccgtc gcccgccgat gtagagggcg atcgtctgca tcgacttgct caccgagatg
     2041 acgggcaggc cggtctgctt ggccacccgc tcggccgtgc ggtgccgggt gccggactcg
     2101 gaggtctcga tccgggagtc ggggacgagt tgggtgttgg cgcggatgat cttggcgacg
     2161 tccttgtcga tgacgacggc gccgtccatc ttggtgagct cccgcagccg ggtcgccgag
     2221 aactcggcgt cgatccggaa cccgcccgag cagatcgcct ccaccgactt gtcgctgccg
     2281 atgacgatga gcgcgccggt gccgctgcgc aggatgcgct caagcccgtc gcgaagctcc
     2341 gtgcccggcg cgacggcagc cagcgtcgcg atgagcaggt cgtcgtcggt tcgttccacg
     2401 gctcggagtc tagctgtggc tgggtcgggg ggcggggtcg acggaggaca cggacgacac
     2461 ggtcggaggc cgccgcgtct cagcgcgatg aggaggacag tgcccggccc accgcctccc
     2521 acacctggct cacttcgatg atccgtatgc cgtccggcat cggcccgctg ccggccactc
     2581 ctcggggaac ccaggcgcgg cggaagccga gcctcgcggc ctcggccagg cgacgcggca
     2641 tacccgcgac tgaccggatc tcccctgcga ggccgatctc gccgaaggcg atcccgtcgg
     2701 agggaaacgg ctcgtcccgc agcgctgaag cgacggcgag ggcgatcgcg aggtcggccg
     2761 cgggctcggt gaccttgacc ccgccaacgg tcgcgacgta ggcgtccttg ccgccgatct
     2821 gggctcctgc gcgctgttgc aggaccgcga ggatcatcgc gatccgggag gcgtccaggc
     2881 cgtgggtcgc tcggcgaggg gtgggcaggg tggaggcggt gaccaggcct tggatctcgg
     2941 cgacgagggg gcggcggccc tcgagggtga cggtgacgca ggtccccgag acggccacct
     3001 ctcggctgga gaggaacagc ccgctcgggt cagggagccc gacgatgccg acgtcggaca
     3061 ggtcgaagca gcccacctcg tcggtcgggc cgtagcggtt cttggcggct cggaccaggc
     3121 gcaaccggga gtgccgctcg ccctcgaact ggacgacgac gtcgacgagg tgctccagca
     3181 ggcgcgggcc ggcgatcgac ccgtccttgg tgacgtgccc gacgagcagc acggcgatgc
     3241 ctcgggtctt ggcgacatgg atgaggctcg cggcgacctc gcggacctgg gcgacgttgc
     3301 cggcggcccc ctccacctgg gcgctggcga tcgtctgcac cgagtcgacg atgacgaggt
     3361 cgggtgtgac cgcctcgatc tggccgagga tcgtggcgag gtcggtctcg gcggcgagga
     3421 agagggacga ggccatggcc tcgatgcgct cggctcgcag ccgcacctgg gcggcggact
     3481 cctcgccgct gacgtagagg acgtctcgtc cggaccgggc cgcgcgggcg gcgacatcga
     3541 gcagcagggt cgacttgccg atccccggct ctccggccac gagcacgacc gcacccggga
     3601 ccaaaccgcc cccaagcacc cgatcgaact cgctgacccc ggtcggcctg gccaccgcgg
     3661 cggtgcggtc cacctcaccg atccgcgcgg gtggcggcac cgcgcgggcg ggggcagtcg
     3721 tgcgtggccg accatcgccc gcctcgccca ccgtccccca ggcctgacat tcgccgcagc
     3781 ggcccaccca cttgaccgtg ctccagccgc actccgagca tcggtatgcc gtgcctcgct
     3841 cgcgggctcg cactgagttg ttggtcgcca tgatcgtgac cctagggcca gggtccgaca
     3901 gcgggtctgt cagaagcggc gccaccacag gttgacggcg tagtccaccc cggtccggtc
     3961 gggatcctcg gccagaatcc gaccggcata cgtgggggcg aactccaacc gcacgaccgc
     4021 ctcgaagtct gagcgcgtgc tcacttccca cgcgacctcg agccgctcct gcacccagcc
     4081 ctgccgacgc cagaaccgct caacagcagc cgggtcgtag tcgggcagtg agccgcggaa
     4141 ccaggcgccg aacgtcgacc gggtcgcgtc gacgtccacg aggaacgcga ggccgccggg
     4201 acgcatgatg cgggtcagct cgaccaggcc tggctcgcac cccggcccga agaaataggc
     4261 ccatcgagcc gtggcgacgt cgaccgactg ccccggcagc ccggtggcct gggccgatgc
     4321 cagtcgcacg ctgacgttgt ccaggccggc agtgcggtcc tgcgccagcg cgaccagcgg
     4381 cgcgtgcggc tccaccccga tgactgcctc ggccgactcg gcgagccgcg gcaggtggaa
     4441 gccggcaccg cagccgatgt cgagcaccgt cgccccggac cagtcggagc ggccggcgag
     4501 ggccagcatg gtcgcgtcga tgaggccggc ccggtcaagg gcgcggttct cgagctcgta
     4561 gatctcgggg gagttccaga tgttaggact gcgaatggcg ccgtcggcgt ccatcgtgtt
     4621 gcccttgacc gtcaacggtt tgcgacgcag ccgccgccgg gactcggcca tcaacgtggc
     4681 cgggtgcacc agcaagggca acagcgcgcg ccccgacccc cgccgcgccg agttgacgct
     4741 gcgcaggtat gccgtgcacc tcaccacaag ttggtcgtag gcggcctcgc ccatgagctc
     4801 gacgagctcg tcgcggttgg accggaacac cggttcgagg ccgacatggg cgtccgagct
     4861 gccggagcag aaccagtcga ggtcgtgccc cccagggccc cacccacgac gatcgaactc
     4921 ggtgatcgaa atctcgagat agctcgtgcc gtcctgcagc tccaccgtgc ggtaggaccg
     4981 gcgaatgggc agctgccagc agacctcggg cttgacctcg tgaggtttgg tcccgtcctt
     5041 gacggcgcgc tggtgcaggg cgcagccggg accggccgcg aagccgggcc ggttgaggaa
     5101 gatgcaggcg ccctcgacga ccttggtctt gcgggcgtcg tcctcccgct cggtccagtt
     5161 gcgctgcgac agtgacccac cggcatacgc gaggtgtcgg aactgccact cgtcctcggt
     5221 gagggcgctc gcagcgtcgc gcacccgctg gtagtcggcc ttctccgtga agtgggcccc
     5281 gagggtgcag cagccgtcgt cggggcggcc ggcatacagg ccacggcagg cgggtgtccc
     5341 gaagacgcac gtccagcggg aggtgagcca cgtgagatcg caccggaagc gttggcgcgg
     5401 gttgtcagga tcggtgaact cgacccacgt tcgggccagg tccagtggag cttcaggcac
     5461 ggcgcacact ctaaccgcgg tcgtcgcggt ccgtggcgct cggtagcgtt cggctatgcg
     5521 cctcggagtg atcgacgtcg gttccaacac cgtccacttc ctcgtggtcg acgcctacta
     5581 cggaggacat ccgctcccgg cctactcgca caaggtcgag ctcaaactca gcgagaacct
     5641 cctgccggac gggacgatcg gggccgagtt cggcagggca ctgactcagt tcctcagcga
     5701 gtgccaggag attgccgagg agcaaggcgt catcgacctc atggcctttg ccacctcggc
     5761 gatccgcgac gcccaaggcg gtcaggagct gatcgagacc gtctcgcggg agtcgggtgt
     5821 cgatctgcaa gtgctcagcg gccgggacga ggcccgcctc acctatctcg cggtgcgccg
     5881 gtggttcggg tggtccagcg gccggctgct cgtcgtcgac atcggtggcg gatcgctcga
     5941 gctggcctcg ggtctggacg aggagcctga tgcggcgatc agcctgccgc tgggcgcggg
     6001 gcggctcacc cgggacctcc tggccgacga cctgcccaaa cccaaggaca tcaaggccgc
     6061 ccgggcctac gtgcgcgccg agatcggcgg cgcggtccgt gagttgctgc gcttcgggcc
     6121 acccacccgc gccgtcggca cgtccaagac gatccgctcg ctggcccgga tcgccggcgc
     6181 ggcgccgagc ggtgagggca tctacgtcac ccggcggctg cggcgcgaac acctcggcga
     6241 catcgtcaag cggctcggtc ggatgtcggt cgccgaacgc gccgaattgc ccggcgtctc
     6301 ggccactcga gcccatcagt tgctcgccgg cgcgctcgtc gtcgagggcg ctatggacct
     6361 gctgcgcctg gaccgactgg acattgctcc ctgggcgctg cgggaaggca tcatcctgcg
     6421 gcgcctggat cacttggaag ccgacgggcc gctgagctga cccggcgcac gcgggccagc
     6481 gggaccgttc acaatgaggg catgcacatc cgggtgagcc tgtcgacggc ttcggtctac
     6541 cccggcacgt gcgcgacggc cttcgagttg gcctcccggc tgggctatga cggcatcgag
     6601 gtcatggtct ggaccgacgt cgtctcccag gaggccggtg ccctgcgcgc cctgtcccgg
     6661 cactacggca tacccgtgct gtcggtccat gcacccacgc tgctcatcac gcaacgcgtc
     6721 tgggggccgg agccctggac caagatcgac cggtcgatcg agttggccgc cgacctggac
     6781 gcgccaacgg tcgtgctgca cccgccgttc cggtggcaga aggagtatgc cgcggagttc
     6841 gtcgacgggg tcgctgcacg cgaggccgag tcgggggtgc gcctggcggt ggagaacatg
     6901 ttcccctggc gggcccgggg ccgcgacctg caggcctacc tcccgcattg ggaccccgtg
     6961 ccgatgagtt acgacaacgt cacgctcgac ctctcgcaca cggcgaccgc cggctcggac
     7021 gccctggcga tggccgccga cctgggggag cgcctgaccc acgtccacct ggccgacggc
     7081 tcggggtcgg tgcgcgacga gcacctcgtt ccgggtcggg gcgggcagcc gtgcgcggag
     7141 ttcctgcagt tgctccccgg ctcggg
//
LOCUS       DAOMZY010000172         7121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_9868_length_7121_cov_6.848288, whole genome shotgun sequence.
ACCESSION   DAOMZY010000172 DAOMZY010000000
VERSION     DAOMZY010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9868_length_7121_cov_6.848288"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="PLK64_10295"
     CDS             complement(<1..827)
                     /locus_tag="PLK64_10295"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV80180.1"
                     /translation="MHLGAYRHVIALPAVRSVLLLGFLVRMPMFATGIVLTLHVVTTL
                     GRSYGEAGLVSAAFTIALAASAPWRGRLLDRHGLRRVVAPSIVATGLVWTTLPFLPYA
                     VLLPASVLGGLFVVPTFSILRQALLAAVPEDRRRTALSLDSVFIELSFMIAPAVAVWA
                     AATYNTQWVLLAVAILNLVAASALYVVNPPITSEAVREVGATVDAPGDKRWVSVGALA
                     VLAAAAATSIVLAGTDVSIVAALRETGDTAAIGWVLSVWGAGSLVGGLVYDAWHRSIS
                     "
     gene            complement(900..2162)
                     /locus_tag="PLK64_10300"
     CDS             complement(900..2162)
                     /locus_tag="PLK64_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HPV80181.1"
                     /translation="MTSVSVVTDTMGDEQRRRGLRQMRTVALGLLLFAAVVYVLTLGK
                     DGVWGFVNAGAEASMVGAIADWFAVTALFRHPMGLPIPHTALIPKKKDMLARSLQDFM
                     GDNFLREEIIRDRVLAAHISARAAAWALVPANSLRATNEVVGVTIAALNRLSDASVTG
                     LLDEAVLPRLREEPIAPIAGTFLGEVVRDRAHHGLVDLAVSEAHRWLLANEETFAAVL
                     EERAPWWAPTRVNEMVIKRLHVEALAWVEDIRDNPHHHARVALDDLLTQLADDLQHDP
                     ETMERAERIKVRVLDHPQLLQTATSLWAAFRTALITSLGESESPLRARIEGQLRAFAE
                     HVRDEAPLQARLDGYAADLATYFVDRYGDELMTVISHTIERWDGQETAEKVELHVGRD
                     LQFIRINGTIVGGLVGVLIHAVSLLLGR"
     gene            2283..3044
                     /locus_tag="PLK64_10305"
     CDS             2283..3044
                     /locus_tag="PLK64_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPV80182.1"
                     /translation="MAQPPTPDRRHLVLVVEDEPAIADLVVLHLHHAGFATHVERDGA
                     AAVEAVARHTPAVVILDIGLPGQDGIEVCRALRSGGDWTPVLFLTARDDHVDRVVGLE
                     LGADDYITKPFSPRELVARVQAVLRRSRPLHPDAELLRSGAVECDLAGRRVLVDGAEV
                     PFTATEFDLLTHLITTPGRVFTRTQLLRAAWGEDALLTLAGERTVDVHVAQVRAKLAP
                     HEVIRTVRGVGYAALTDRERADLPGHTDSEPRGRA"
     gene            3041..4453
                     /locus_tag="PLK64_10310"
     CDS             3041..4453
                     /locus_tag="PLK64_10310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPV80183.1"
                     /translation="MKGLLSTLGARVTALTLAVVLATATVGSIAAVLLVQRANDAAAA
                     RSLAATADVAQSLATLATSPELGLARAKRALDGVQVQVAIVRSGTPRGTVVTGDPLPR
                     RLLTEAVLAQLVAGQDVSLRVPDDDGQVFLEGRATEAGAIVLAQRRSDAVALGQDAVA
                     RMRWAFALVALVSVLVGWAVSRRMTTPLRRMAAAAGDLASGARDVAVPESGPAEVAAV
                     GAALNELARSLAHSEARQREFLLSVSHDLRTPLTAVTGYAESLADGVIPPEGVAEVGR
                     IVGAEAARLDRLVQDLLDLARLDAHEVTLTMAAVDLAGLLASAEPVWRRRCDAVGVAF
                     GLELPAVPLVVTADPDRLRQALDGLVDNALRATPAGRPVIVGAHTESGYAVLEVRDGG
                     PGLAPEDFPVAFERSVLHERYRGVREVGTGLGLAIVARIVARHGGTVAARPAPEGGAC
                     FAITLPVATPIVPTTSPVGQ"
     gene            4503..4781
                     /locus_tag="PLK64_10315"
     CDS             4503..4781
                     /locus_tag="PLK64_10315"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HPV80184.1"
                     /translation="MSTVGIRELRQEASRHVAAAAAGAAVTVTDRGVPVAKLVPLSPL
                     EHHLAQMLARHGIRPPARPRRRFVGDLRLTGPSLTAEILDARAERDAW"
     gene            4775..5197
                     /locus_tag="PLK64_10320"
     CDS             4775..5197
                     /locus_tag="PLK64_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPV80185.1"
                     /translation="MVIYLDTSALAKLVVEEAESADLAAWLDARPDTPLVTSTLTQVE
                     LLRAARRRDGSTVPRAIALLAELALIPLDDPVLDAAVLQDPAELRTLDALHTASAALL
                     GAELDALVTYDERMAVAATAVGIPVVSPGSTSAASTPR"
     gene            complement(5209..6045)
                     /locus_tag="PLK64_10325"
     CDS             complement(5209..6045)
                     /locus_tag="PLK64_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HPV80186.1"
                     /translation="MTADDGHGHGDAHGDGHGHGHGEAVPVDSSGMPWAHRQLSPSGF
                     EDDDGAADPLVREAAIAVAAEPSSAGELALMAAVATARWLVPMVATGVDTEVADGRRV
                     DGHAEMAYVSLTAPDGQRALPVFSGLDALAIWDPAARPVPVTADRAAQAAVSEGCDTL
                     LLDLGSPHAVALRPSMVWALAMAREWFPAHDDPTVRAGVTAAAALEPDVEAVDISDGT
                     PVASGILRIALTLRPGLAASDVSALATRIGERIASDGELRARIDGIAFAVRAESPAAS
                     SE"
     gene            complement(6042..6779)
                     /gene="priA"
                     /locus_tag="PLK64_10330"
     CDS             complement(6042..6779)
                     /gene="priA"
                     /locus_tag="PLK64_10330"
                     /EC_number="5.3.1.16"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase/phosphoribosylanthranilate isomerase
                     PriA"
                     /protein_id="HPV80187.1"
                     /translation="MTESPRLQLLPAVDISDGGAVQLQQGVVGSGWKFGDPWLAAKRW
                     QDDGAEWIHLVDLDAAFGRGNNRELIADIVGRLDLQVELSGGIRDADSLAVALATGCR
                     RVNVGTAALENPEWTARAIAEHGDRVAVGLDVRGTRLAARGWTSEGGDVWETLDRLDA
                     EGCSRYVVTDVTKDGMLAGPNLALLREVAARTDRPVIASGGVSTLADIAAIRALVPEG
                     VEGAIIGSALYKGAFTLPEALDLAGRP"
     gene            complement(6776..>7121)
                     /locus_tag="PLK64_10335"
     CDS             complement(6776..>7121)
                     /locus_tag="PLK64_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80188.1"
                     /translation="VPMNAKRASGTRRTTATRRRRSTTTSSRYRRRRRPGLSSTVGSA
                     IGMLAVTTLLNLSWPARIGLLVLVLVVGLAYMLWRHRAEIAPGADGIPEDAAAPPTPQ
                     DRPTPSPEENPS"
BASE COUNT         1044 a         2565 c         2492 g         1020 t
ORIGIN      
        1 gagatcgacc gatgccaggc gtcgtagacc agcccaccga ccaaggatcc cgcgccccac
       61 acgctcaaga cccagccgat cgcggcagtg tccccggttt cacgcagggc ggcgacgatg
      121 ctgacgtcgg ttccggccag cacgatactc gtcgccgcgg cggctgcgag gacggccagc
      181 gccccgacgc tcacccagcg cttgtcgccg ggcgcgtcga ccgtcgcccc gacctcgcgc
      241 acggcttcgg acgtgatcgg cgggttgacg acgtagagag cggaagcggc gaccaggttc
      301 aggatggcca ccgcgagaag gacccattgg gtgttatagg tcgcggcagc ccacaccgcc
      361 acggctggag cgatcatgaa cgacaactcg atgaagaccg agtcgagcga gagggccgtg
      421 cggcggcggt cctccggcac cgcggccagc aacgcctgac gcaggatcga gaaggtcggc
      481 acgacgaaca gcccaccgag caccgatgcc ggaagcagca cggcatacgg gaggaacggc
      541 agggtcgtcc acacgaggcc cgtggcgacg atcgacgggg cgacgacccg acgcagaccg
      601 tggcggtcca gcagccgccc tcgccagggg gcggacgccg cgagcgcgat ggtgaaggcc
      661 gcggacacca gccccgcctc gccgtaggaa cgacccagcg tggtgaccac atggagggtg
      721 agcacgatgc ccgtggcgaa catcggcatc cgcacaagga agcccaacag cagcaccgac
      781 cgcaccgccg gaagggcgat gacgtgacgg taggcgccta agtgcatcag agcagtttct
      841 caccaccggc acgcgccacc caaccgagtt actcggtatg ccgcccgctc accacgacct
      901 caccgaccca gcaggagcga caccgcgtgg atgagcacgc cgacgagccc gccgacgatc
      961 gtgccgttga tccggatgaa ctgcaggtca cgccccacat ggagttcgac cttctcggcc
     1021 gtctcctggc catcccagcg ctcgatggtg tggctgatga cggtcatcaa ctcatcgccg
     1081 tagcggtcga cgaagtaggt ggcgaggtcg gcggcatacc cgtcgagccg ggcctgcagg
     1141 ggagcctcgt cgcgcacgtg ctcagcgaac gctcgcaact gcccctcgat ccgcgcgcgc
     1201 agcggcgact cgctctcccc caacgacgtg atcagcgccg tgcggaaggc cgcccagagc
     1261 gaggtcgcgg tctgcagcag ctgcgggtgg tcaagcacgc ggactttgat ccgttcggcc
     1321 cgctccatcg tctccgggtc gtgctggagg tcatcggcga gttgggtgag caggtcgtcc
     1381 agggcgaccc gagcatggtg gtgcgggttg tcgcggatgt cctcgaccca ggcgagcgct
     1441 tcgacgtgca gtcgcttgat gaccatctcg ttgacccgcg tcggcgccca ccacggggcg
     1501 cgttcctcga ggaccgccgc gaaggtctcc tcgttggcga gcaaccaccg gtgggcttcg
     1561 ctcaccgcga ggtcgaccag cccgtggtgc gcgcggtcgc gcaccacttc gccgaggaag
     1621 gtgccggcga tgggcgcgat cggctcctcg cgcagtcgcg gcagcaccgc ctcgtccagc
     1681 agcccggtca ccgatgcgtc gctgagccgg ttgagcgcgg cgatcgtcac gccgacgacc
     1741 tcgttggtgg cccgaaggga gttggccggc acgagggccc aggccgcggc ccgcgccgag
     1801 atgtgggcgg ccagaacccg gtcgcggatg atctcctccc gcaggaagtt gtcacccatg
     1861 aagtcctgca ggctccgggc cagcatgtcc ttcttcttgg ggatgagcgc ggtgtgcggg
     1921 atcggcagac ccatcgggtg ccggaacagc gccgtcaccg cgaaccagtc ggcgatcgcg
     1981 ccgaccatcg atgcctcggc gcccgcgttg acgaaccccc agacgccgtc tttgcccagg
     2041 gtgaggacat agacgaccgc ggcgaacagc agcagaccca acgccacggt gcgcatctgc
     2101 cgcagccctc ggcgtcgctg ctcgtcgccc atcgtgtcgg tcacgacgct cacactcgtc
     2161 atgagtgtcc cttctatcgg ccggtcaacc gggcgccctg acacaaccgc cctgacacaa
     2221 ccctgacacg accgagacgc cgccgtgacc gcgccccttc ctgcggctcc tagagtggcg
     2281 ctgtggcgca gcctcccacc cccgaccgac ggcacctggt gctcgtcgtc gaggacgagc
     2341 cggccatcgc ggatctcgtc gtcctgcacc tgcaccatgc cgggttcgca acccacgtgg
     2401 agcgcgacgg tgcggcggct gtggaagccg tggcacggca tacccccgcg gtggtgatcc
     2461 tcgacatcgg cttgcctggg caggacggca tcgaggtatg ccgcgcgctc cggtccggcg
     2521 gcgactggac ccccgtgctg ttcctcaccg cgcgcgacga ccacgtcgac cgggtggtcg
     2581 ggctggagtt gggagcggac gactacatca ccaagccgtt ctcgccgcgc gagctggtcg
     2641 cgcgggtgca ggcggtgctg cgccgcagcc gacctctgca ccccgatgcc gaactcctgc
     2701 gctccggcgc cgtcgagtgc gatctggccg ggcgtcgggt cctcgtcgac ggagccgagg
     2761 ttcctttcac agcaacagaa ttcgacctgc tcacccatct gatcacgacg cccgggcggg
     2821 tcttcaccag gacccaactg ctgcgggccg cctggggcga ggacgccttg ctcaccctcg
     2881 ccggcgagcg caccgtcgat gtccacgtcg cccaggtgcg cgccaaactc gcccctcacg
     2941 aggtgatccg caccgtccgc ggagtcgggt atgccgccct gaccgaccgc gagcgcgccg
     3001 acctccccgg ccacaccgac tccgagcctc ggggccgggc gtgaagggtc tgctgtcgac
     3061 cctgggcgcg cgggtcacgg cgctcaccct ggccgtcgtc ctggcgacgg ccaccgtggg
     3121 gtcgatcgcc gcagtcctgc tcgtccaacg ggccaacgac gcggccgccg cccgttcgct
     3181 ggccgcgacg gccgatgtcg cccagtcgct ggcgacgctc gcgacctcac ccgagttggg
     3241 tctggcgcgg gccaaacggg ccttggacgg cgtgcaggtt caggtcgcca tcgtccgatc
     3301 cggcacccct cggggcacgg ttgtgaccgg agacccgctc ccccgacgcc tgctcaccga
     3361 ggcggtgctg gcgcagttgg tcgcgggtca ggacgtgtcc ctgcgggttc cggacgacga
     3421 tggccaggtc ttccttgagg gccgggccac cgaggccgga gctatcgtgc tggcgcaacg
     3481 ccgcagcgat gcggtcgcgc tcggccagga cgccgtggca cggatgcggt gggcgttcgc
     3541 cctcgtcgcg ctggtgtcag tgctcgtcgg ctgggcggtg tcccgtcgca tgaccacgcc
     3601 cctgcgccga atggcagcgg cggctggcga cctcgcctcg ggtgcccgcg atgtcgccgt
     3661 gccggagagc ggccctgccg aggtggccgc cgtgggcgcc gcgctcaacg agttggcccg
     3721 atcgctggcg cactcggagg cccggcagcg cgagttcctc ctgtccgtct cgcacgactt
     3781 gcgcactccg ctcacggccg tcaccgggta tgccgagtcg ctcgccgatg gcgtcatccc
     3841 tccggagggc gtggccgagg tcggacggat cgtcggggcc gaggccgcgc gtctggaccg
     3901 gctcgtccag gatctgctcg atctggcccg cctggacgct cacgaggtca ccctcacgat
     3961 ggccgcggtc gacctggcgg gtctgctcgc gtcggcagaa ccggtgtggc gccgccgctg
     4021 cgacgccgtc ggcgtcgcgt ttgggctgga gctccccgcg gtgccactgg tcgtcaccgc
     4081 agaccccgac cgcttgcgcc aggcactcga cggcttggtc gacaacgccc tgcgcgccac
     4141 tcccgccggt cggcccgtca tcgtgggtgc tcacaccgag agtgggtatg ccgtcctcga
     4201 ggttcgcgac ggcggccccg gcctggcgcc ggaggacttc ccggtggcgt tcgagcgatc
     4261 ggtgctgcac gagcgctatc gaggggtccg cgaggtcggg accggtctcg ggctggcgat
     4321 cgtggcccgg atcgtcgccc gccacggcgg cacggtggcg gcccggccag cgccggaggg
     4381 tggcgcctgc ttcgcgatca cccttcccgt cgcgacaccg atcgttccca ccacctcacc
     4441 ggttggacag tgaccccaac ctcggtggtt ctttgtagca catggagtgc tactctcggc
     4501 agatgagcac cgtcggcatt cgcgagctgc gccaggaagc cagccgccat gtcgcggcag
     4561 cggctgcggg ggcagccgtc acggtgaccg atcgaggcgt cccggtggcc aagttggttc
     4621 cgttgtcccc tctggaacac cacctggccc agatgctggc acggcatggc atccggccac
     4681 cggctcggcc acgtcgacgg ttcgttggtg acctccgatt gaccggcccg agtttgacgg
     4741 ccgagatcct ggatgcgcgg gccgaacgcg acgcatggtg atctatctcg acacgtcggc
     4801 cttggcgaag ttggtcgtgg aggaggccga atcggctgac ctcgcggcgt ggttggacgc
     4861 gcgcccagac acgcctctcg tcacgagcac cctgactcag gtcgagttgc ttcgggccgc
     4921 ccgacggcgg gatggatcga cggtgccgcg cgccatcgcc ctgctggcag agctggcact
     4981 catcccactg gacgacccgg tccttgatgc ggccgtcctc caggacccag ccgagctgcg
     5041 cacgctggac gccctccaca ccgccagcgc ggccctgctc ggagccgagt tggacgcgct
     5101 cgtcacctac gacgagcgga tggcggtggc agccacggcg gtcggcatac ctgtggtgtc
     5161 ccccgggtcg acctcggcgg cctcgacgcc gcgttagtca gcgcagggtc actcagacga
     5221 agcggccggc gattcggcgc gaacggcgaa ggcgatcccg tcgatccggg cgcgcaactc
     5281 cccgtcggag gcgatccgct caccgatccg ggtggccaat gcagagacgt ctgaggctgc
     5341 tagtcccggc cggagcgtca gggcgatccg caggatgccc gaagcgacgg gcgtgccgtc
     5401 gctgatgtcg acagcctcga cgtcaggctc cagcgccgca gcggcggtca cgcctgcgcg
     5461 gaccgtcggg tcgtcgtggg ccggaaacca ctcccgcgcc atagccaatg cccagaccat
     5521 gctggggcgc agcgcaacgg catgaggtga cccgaggtcg agcagcaagg tgtcgcagcc
     5581 ttcgctgact gccgcctgag ccgctcggtc ggccgtcacc ggaaccgggc gggccgccgg
     5641 gtcccagatc gcgagcgcgt ccagcccgct gaacaccggg agggcgcgct gcccgtcggg
     5701 tgcggtgagc gagacgtagg ccatctcggc gtgaccgtcg acccgacgcc cgtccgcaac
     5761 ctcggtgtcc acgccggtcg ccaccatcgg aaccagccat cgggccgtcg ccaccgcagc
     5821 catgagcgcg agctccccgg cgctgctcgg ctcggcagcc acggcgatgg cggcctccct
     5881 cacgagcggg tcggcggcgc cgtcatcgtc ctcgaaaccc gacggggaca actggcggtg
     5941 cgcccacggc ataccggagg agtcgaccgg aacggcctca ccgtgcccgt gcccgtgtcc
     6001 gtcgccgtgt gcatcgccgt ggccgtggcc gtcgtctgcg gtcacggacg gccggccaga
     6061 tcgagcgcct ccggcagggt gaacgcgccc ttgtaaagcg ccgatccgat gatcgcgccc
     6121 tcgacccctt caggcacgag agcgcgaatc gccgcgatgt cggccagggt cgagacgccg
     6181 ccggaggcga tgactggccg gtcggtccgg gccgcgacct cacgcagcag ggcgaggttg
     6241 gggccggcca acatgccgtc cttggtgacg tcggtgacga catagcgcga gcagccctcg
     6301 gcgtcgagcc ggtccagggt ctcccacacg tcgccgccct ccgaggtcca accgcgcgcg
     6361 gccagccggg tgccacggac gtcgaggccg acggcgaccc ggtcgccgtg ctcggcgatc
     6421 gcgcgggcag tccactccgg gttctccaag gcggcggtgc cgacgttgac ccgacggcag
     6481 ccggtcgcga gggcaacggc cagcgaatcg gcgtcgcgga tcccgccgga gagttccacc
     6541 tgaaggtcca gccggccgac gatgtcagcg atcaattcgc ggttgttccc acggccgaag
     6601 gccgcgtcga gatcgaccag gtggatccac tcggcgccgt cgtcctgcca acgcttcgcg
     6661 gccagccagg ggtcgccgaa cttccacccc gagccgacga cgccctgctg cagttgcacg
     6721 gcacctccgt cggagatgtc gacggcgggc agcagttgca gtcggggcga ctcggtcacg
     6781 acgggttctc ttccgggctg ggggtgggtc ggtcctgcgg ggtgggtggt gccgctgcgt
     6841 cctcgggtat gccgtccgcc cccggcgcga tctcggcccg gtgccgccag agcatgtagg
     6901 ccaggccgac cacgaggacc agcacgagca gcccgatccg agccggccac gacaggttga
     6961 gcagggtcgt cacggcgagc atcccgatcg ccgagccgac cgtcgacgac aggccgggac
     7021 ggcgacgacg ccgataccgc gaggacgtcg tcgtcgacct gcgtcgcctg gtggcggtcg
     7081 tgcggcgggt gccggaggcg cgcttggcgt tcatagggac c
//
LOCUS       DAOMZY010000173         7102 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_9909_length_7102_cov_6.634880, whole genome shotgun sequence.
ACCESSION   DAOMZY010000173 DAOMZY010000000
VERSION     DAOMZY010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7102
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9909_length_7102_cov_6.634880"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="PLK64_10340"
     CDS             <1..679
                     /locus_tag="PLK64_10340"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPV80189.1"
                     /translation="SLAEVPAARKLWSQRRTEFWLSIAATLAVAVMGVLPGIGVAVGL
                     SILNVFRRAWWPYQAVLGREKGHPGYHDMTHRPRLTSMPGLIIFRFDAPLFFANARTF
                     GEQITRLAATEPAPKWILVAAEPISDIDTTAADMLASLVADLDERGTKLAFAELKSVV
                     RGKLEAYALDGVIDNVLYFPTLNAAGKAYRAQFGTDWGDTDFPAVWPPPPGSVPATSA
                     SPTDPDE"
     gene            690..1370
                     /locus_tag="PLK64_10345"
     CDS             690..1370
                     /locus_tag="PLK64_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HPV80190.1"
                     /translation="MAHLIAVNVGTPRPDGDSEGPPTGIHKSPVEGPVDLRDPGDKGT
                     GLGSGVAGDFLGDRRHHGGTDQAVYAVARDELAHWAAEVGRDLPPGAFGENLTITGLD
                     VDGALVGEIWAIGDVVRLQVTGPRIPCATFARAMGEERWVKRFTVRGRTGAYLRVLTP
                     GPVQAGDAVVVESRPDHEITVPLMFRALTTERHLLPRLAEVGPDLSEEGRRELAAYRE
                     GTTDGRMP"
     gene            1473..1670
                     /locus_tag="PLK64_10350"
     CDS             1473..1670
                     /locus_tag="PLK64_10350"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HPV80191.1"
                     /translation="MKLNVGTTDRKIRIAIAIVAAILAVVVGGTFGVVLWVVAAIMLA
                     TGIVRVCPLYLPFKISTNKGA"
     gene            1740..2549
                     /locus_tag="PLK64_10355"
     CDS             1740..2549
                     /locus_tag="PLK64_10355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPV80192.1"
                     /translation="MGITHEEITAAVPGLLRYARVLTRDAATAEDLVQDTVVRALERA
                     ADFRGDSSAATWLHRILHHRFVDVVRASRSDPVDAEELAERVEADWRDDSYTVDAETV
                     LLRAETAEELRDAIIHLPAAYRSAVVLHDVQGLTSAAVADIQDVSLAAAKQRISRARA
                     MLVTHLARGTERRAALGGIPLRCWDARSRVSDYLDGDLDSATRVALETHLGSCPTCPA
                     LYAGLVGVRAALGALRDPDTVVPPALAARLRAAEQASVAKDVAKDSGQARR"
     gene            2515..3822
                     /locus_tag="PLK64_10360"
     CDS             2515..3822
                     /locus_tag="PLK64_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HPV80193.1"
                     /translation="MSQRIRAKPGGDMGEFRLDGLMLGVATAATQIEGGCDTTNWADW
                     ALTPGHIADGSSPVRATGHWDRWREDTDLMASLGIQTYRFGVEWARLEPQPGVFSDEA
                     FSHYRDELTALRDKGIRPLLTLHHFNNPRWFEALGAFERADNVAHFLRFVERVVTELG
                     DLVEDWCTINEPNVYATFGYLLGRFPPGVRSLPRAVRVMTNLAHAHITAYELIHALRA
                     GQPTRVTFAVHLRVFDPANPANPAHRALARAQEYLFQTALTEAMCTGRFGMPLRRPAG
                     IHEGRYYDALGINYYSRSWVSTIGDGTRPGAQLNDLGWEIYPEGLARVARWAYDRWPA
                     PIWVTENGTCDATDRFRAQYLHDHLEVMAAARATGLPFERYYHWCFVDNWEWAEGEVP
                     RFGIVDLDYATLERTVKSSGRFYADVIAEGGVTDEIAARYLSS"
     gene            complement(3807..5402)
                     /locus_tag="PLK64_10365"
     CDS             complement(3807..5402)
                     /locus_tag="PLK64_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPV80194.1"
                     /translation="MPVESAGPSAGADSGADLDRCPQVLLRNVRVVPVGGVPAPQGSV
                     DVRMRAGRVVDLAPTVTPDPHDSEVHDGEGRWIIPGLWDHHVHLAQWADTLSRLDLSG
                     TTSPDEVTDRIRRHLDTLPVNDADSVVVGFGHRSATWARQPTVAELDAVSGRHPVALV
                     SGDGHNGWLNSAALALLGAPATEGALVENDWYPVWTRIGSLPGSQEARDAAYRLAVSR
                     AAALGIVGVGDMEFGDGFLDWPRRFAAGVQDLRVRVATYPTGLDAVLAAGLRSGDVLG
                     DSGGLVRMGPLKIISDGSLNTRTAYCHEEYAGGSGLAEPRGRANYSGADLAELARRAT
                     QAGLRMAVHAIGDAAITQALDVFESTGARGTIEHAQLMQPSDVVRMARLGVGASVQPA
                     HLLDDRDVTALCWPDRADRCFPLREMVEAGVALLLGSDAPVSPLDPWLAMAAAVHRSA
                     DERDPWNAGQSLTAAQALAGSTDGCTTVTLGSRADLVVLEADPLDGAAGDSAAVGQRL
                     RTMPVAATYLGGRRTFGSGHDDR"
     gene            5540..6163
                     /locus_tag="PLK64_10370"
     CDS             5540..6163
                     /locus_tag="PLK64_10370"
                     /inference="COORDINATES: protein motif:HMM:NF040060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6318 family protein"
                     /protein_id="HPV80195.1"
                     /translation="MSSPITRHAVGRYAVGLAAVALVGACSSGPSAATATSGATAATS
                     ARSTAGATATVRPTATAAATPTYPSDVPAAAQANTPAGALAFTRYFFGRLNAAYATGQ
                     TGLLRPLGTAKCTGCGTLEDEAAAVTSGGRHTVSEPMLLREVTASNEPHIEGQTPVDV
                     LFRLRATKIVDSAGAAAGTLAEKNGIYLVSLLRDGVVWRVSSITLMQ"
     gene            6406..>7102
                     /locus_tag="PLK64_10375"
     CDS             6406..>7102
                     /locus_tag="PLK64_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80196.1"
                     /translation="MLISPAGPRIRAMRRRILAVLAVMALAVVGLQALPAHAAAPPPM
                     TPSITSTVSTIDVNGCGAARGFSRYLVPNLGFEEDCNMHDLCYGSGLPRAGCDDRFAE
                     SMSDTCQSNPICRAAAALYAEAVRKAGEGAYREATQQRLDEFINNIVACGDDRACEER
                     VRQEQAMDALVNELTACNGNRACEQEVIDRRVPKEEPKKDDPPAATSDEAPAADEPEA
                     ANPETQEPATENPP"
BASE COUNT         1105 a         2592 c         2389 g         1016 t
ORIGIN      
        1 ctcgctggcc gaggtccccg ctgcccgcaa gctctggtca cagcggcgaa cggagttctg
       61 gctgtcgatc gccgcgaccc tggccgtggc cgtcatgggc gtcctccccg gcatcggggt
      121 cgccgtcggg ctgtcgatcc tcaacgtgtt ccgccgggcc tggtggccgt atcaggcggt
      181 gctcggccgg gagaagggcc acccgggcta ccacgacatg acgcatcggc cgcggctcac
      241 gtcgatgccg gggctcatca tcttccgctt cgacgcaccg ctgttcttcg ccaacgcccg
      301 gaccttcggg gagcagatca cccgactggc cgcgaccgaa ccggcaccca agtggatcct
      361 cgtcgcagcc gagccgatct ccgacatcga cacaacggcc gccgacatgc tcgccagcct
      421 ggttgccgat ctggacgagc gcgggacgaa actggccttc gccgagctca agagcgtcgt
      481 caggggcaag cttgaggcct acgcgctcga cggggtcatc gacaacgttc tctacttccc
      541 caccctcaac gcggcgggga aggcctatcg ggcgcagttc gggaccgact ggggcgacac
      601 tgacttcccg gccgtctggc cgccgcctcc cggttccgta cctgccacat cggcctcgcc
      661 cacggatccc gacgagtgaa ggactcacca tggcccacct gatcgccgtc aacgtcggca
      721 ccccgcgacc cgacggggac agcgaagggc cgccgaccgg catacacaag tcgcccgtcg
      781 agggacccgt ggacctgcgc gaccccgggg acaaggggac ggggctcggc agcggggtcg
      841 ccggcgattt cctcggggac cggcgccacc acggcggcac cgaccaggcg gtgtatgccg
      901 tcgcgcgcga cgagcttgcg cactgggctg ccgaggtggg tcgggacctc ccgcccggag
      961 cgttcggcga gaacctcacg atcaccggcc tcgatgtcga cggtgccctc gtcggggaga
     1021 tttgggccat cggtgacgtg gtccgcttgc aggtgaccgg tccgcgcatc ccgtgtgcga
     1081 ccttcgcgag ggccatgggg gaggaacgct gggtcaagcg cttcacggtg cgcggccgca
     1141 ccggcgccta cctgcgcgtc ctcacgccgg gcccggtgca ggctggcgac gcggtcgtcg
     1201 tcgagagccg acccgaccac gagatcaccg tgccgctgat gttccgggcg ctgacgaccg
     1261 agcggcacct gttgccgcgg ctcgcggagg tcggtcccga cctgagcgag gagggccgcc
     1321 gggagctcgc ggcctaccgc gaggggacca ccgacggccg gatgccgtag cgcatcgagt
     1381 gtgacccacg acatacccgg ttcctgtcac ctttgcgcgg tccgcaccgt ccatgcggcg
     1441 ttcgaccctc tgacctaagg aaacgacgca ccatgaagct caacgtcggc accaccgacc
     1501 ggaagatccg cattgccatc gccatcgtcg ccgcgatcct cgcggtcgtc gtcggcggca
     1561 ccttcggggt ggtcctgtgg gtcgtcgcgg cgatcatgct cgccaccggc atcgtccgcg
     1621 tgtgcccgct ctacctcccc ttcaagatca gcaccaacaa gggcgcctga caccctccgg
     1681 tatgccgtcc gcccgcctcc cgctcaccgc ggggggaccg gacggcatac cgttgtcgca
     1741 tggggatcac gcacgaggag atcacggccg ccgtccctgg cctgttgcgc tacgcgcgcg
     1801 tcctgacccg ggatgccgcg acggccgagg acctcgtgca ggacaccgtg gtgcgcgccc
     1861 tggagcgcgc ggccgacttc cgtggtgact cctccgccgc gacctggctc caccggatcc
     1921 tgcaccaccg gttcgtcgac gtcgtccggg cctcgcgctc cgaccccgtc gatgccgaag
     1981 aactcgccga acgcgtcgag gccgactggc gcgacgattc ctacaccgtc gacgccgaga
     2041 ccgtactcct gcgggctgag accgccgagg aactgcgcga cgcgatcatc catctgccgg
     2101 cggcctaccg atcggctgtc gtcctgcacg acgtccaggg cctgacctcg gcggcggtcg
     2161 ccgacatcca ggacgtcagc ctggccgccg ccaaacaacg gatcagccgg gctcgtgcga
     2221 tgctcgtcac ccacctcgca cgcggcaccg aacggagggc ggccttgggc ggaatcccct
     2281 tgcgctgctg ggacgctcgc tcgcgtgtga gtgactacct cgacggagat ctcgactcgg
     2341 ccacgcgcgt cgcactcgaa acgcacctcg gctcgtgccc cacctgcccc gccctgtatg
     2401 ccggtctcgt cggcgtgcgc gccgcgctcg gcgccctgcg cgaccccgac acggtcgtcc
     2461 ccccggccct cgccgcacgg ctccgggcgg ccgagcaggc cagcgtcgca aaggatgtcg
     2521 caaaggattc gggccaagcc cggcggtgac atgggcgagt tccggctcga cggcctgatg
     2581 ctcggcgtcg ccaccgcggc cacccagatt gagggtggct gcgacacgac gaactgggcc
     2641 gactgggcgt tgactcccgg gcacatcgcc gacggatcct cgcccgtccg ggccacgggg
     2701 cactgggatc ggtggcgcga ggacaccgat ctgatggcct cactcggcat ccagacctac
     2761 cgtttcgggg tcgagtgggc tcggctggag cctcagccgg gcgtcttctc cgacgaggcc
     2821 ttcagccact atcgcgacga gctgactgcc ttgcgggaca agggaatccg gcctctgctc
     2881 acgttgcatc acttcaacaa cccccggtgg ttcgaggcgt tgggagcctt cgagcgagcc
     2941 gacaacgtgg cgcacttcct gcgcttcgtc gagcgggtcg tcaccgagct cggcgacctc
     3001 gtcgaggact ggtgcacgat caacgaaccc aacgtctatg cgacgttcgg ctacctcctc
     3061 gggcggttcc ccccgggcgt ccggtcgctg ccgcgagccg tgcgggtgat gaccaacctc
     3121 gcgcacgccc acatcacggc atacgagctc atccacgcgt tgcgcgcggg tcagccgacg
     3181 cgcgtcactt tcgcggtcca tctgcgcgtc ttcgacccag ctaacccggc caaccccgcg
     3241 caccgcgccc tcgcgcgggc ccaggaatac ctcttccaga cggccctcac cgaggcgatg
     3301 tgcacgggcc gattcggtat gccgctgcgc cgccccgcgg ggatccacga ggggcgctac
     3361 tacgacgcgc tcgggatcaa ctactactcg cgctcctggg tgagcacgat cggcgacggg
     3421 actcggccgg gggcgcagct caatgacctg gggtgggaga tctaccccga ggggctggcg
     3481 cgcgtggcgc ggtgggccta tgaccggtgg ccggccccga tctgggtcac cgagaacggc
     3541 acctgcgacg ccaccgaccg cttccgtgcc caatacctgc acgatcacct cgaggtgatg
     3601 gccgcggcgc gcgcgaccgg cctgccgttc gagcgctact accactggtg cttcgtcgac
     3661 aactgggagt gggccgaagg cgaggtgccg aggttcggga tcgtggacct ggactacgcc
     3721 accctggagc gcacagtgaa gtcgagcgga cgcttctatg ccgacgtcat cgccgagggc
     3781 ggcgtcaccg acgagatcgc cgcgcgctac ctgtcgtcgt gacccgagcc gaacgtccgc
     3841 ctgccgccga ggtaggtggc ggccaccggc atcgtccgca gccgctgccc caccgcggct
     3901 gagtcgccag ccgctccgtc caacggatca gcttccagca caacgagatc cgcacggctg
     3961 cccagcgtca cggtggtgca gccgtcggtg ctgccggcca gcgcctgcgc cgcagtgaga
     4021 gactgcccgg cgttccacgg gtcacgctcg tccgcgctgc ggtgcacagc cgccgccatc
     4081 gccagccacg ggtccagcgg cgacaccggc gcgtcggagc cgagcagcag cgcgacgccg
     4141 gcctcgacca tctcacgcag ggggaagcac cgatcggctc gatcgggcca acacaacgcg
     4201 gtgacgtcgc ggtcgtccag cagatgggcc ggctgcacac tggcccccac gccgaggcga
     4261 gccatccgca cgacatccga cggctgcatc aactgcgcgt gctcgatggt cccgcgggcg
     4321 cccgtcgact cgaacacgtc caacgcctgc gtgatcgccg cgtcaccgat ggcgtgcacc
     4381 gccatccgca ggcccgcctg ggtcgctcgc cgggccaact cggcgaggtc cgcaccggag
     4441 tagttcgcgc gaccacgcgg ctccgcgaga ccggacccac cggcatactc ctcgtggcag
     4501 taggccgtgc gggtgttgag cgagccgtcc gagatgatct tcagcggtcc catccgcacc
     4561 agcccgcccg agtcgccaag gacgtcgccg ctgcgcaggc cggctgcgag caccgcgtcc
     4621 agcccggtgg ggtaggtcgc cacacggacc cgcaggtctt gaaccccggc cgcgaatcgc
     4681 cttggccagt cgaggaatcc gtcgccgaac tccatatcac cgacgccgac gatccccagc
     4741 gcggcggcac gcgacaccgc gagtcggtat gccgcgtccc tcgcctcctg cgaccccggc
     4801 aggctgccga tccgggtcca cacgggatac cagtcgttct cgacgagggc cccttcggtc
     4861 gccggtgcgc cgagcagggc gagcgccgcc gagttgagcc acccgttgtg tccgtcaccg
     4921 ctgacgagag cgaccgggtg ccgcccggac acggcgtcga gctcggccac agtgggctgg
     4981 cgggcccagg tcgccgaccg atgaccgaag ccgacgacca cactgtccgc gtcgttcacc
     5041 ggcagtgtgt cgaggtgtcg acggatccgg tcggtgacct cgtctgggct cgtcgtgccc
     5101 gacaggtcga gacgcgagag ggtgtcggcc cactgggcca ggtggacgtg gtgatcccac
     5161 agcccaggga tgatccaccg gccctcgccg tcgtggacct cgctgtcgtg cgggtccggc
     5221 gtgaccgtcg gggccaggtc gacgacgcga ccggcgcgca tccgcacgtc gacgctcccc
     5281 tgcggtgccg gcaccccgcc gaccggaacg acccgcacgt tgcgcaggag cacctggggg
     5341 cagcggtcga ggtcggcacc ggagtcagcc ccggcgctcg gcccggcgga ctcgacaggc
     5401 atgcggaggc ctttcgcgcg gggagcagac gccctcacgc tagcgcccga accggccgct
     5461 cgcctcgccc tgcgggggca ccgagacgcc catgggagac ttgggcgcag gggaaaccag
     5521 ccgcgcaggg aggatgtcga tgtcgtcgcc gatcacgagg catgccgtcg gaaggtatgc
     5581 cgtcgggctg gctgccgtcg cactcgtcgg agcctgctcg tcaggcccca gtgctgccac
     5641 ggccacgtcc ggcgccaccg ccgcgacgag tgcccgatcg acggctggcg ccacggccac
     5701 ggtgcgcccg accgcgaccg ccgcggcgac gccgacctat ccgagcgacg ttccggccgc
     5761 agcccaggcc aacaccccgg ccggagcgtt ggcgttcacg cgctacttct tcgggcggct
     5821 caacgcggca tacgccacgg ggcagacggg actgctgcgt ccgctgggca cggcgaagtg
     5881 cacagggtgc gggacgctcg aggacgaggc ggcagcggtg acgagcggcg ggcggcatac
     5941 cgtgtccgag ccgatgttgt tgcgagaagt gacggcgagc aacgaaccgc acatcgaggg
     6001 ccagacgccg gtcgacgtgt tgttccgact gcgcgcgacc aagatcgtcg attccgcagg
     6061 agcggcggcc gggaccctgg cggagaagaa cgggatctat ctcgtgtcac tgctgcgtga
     6121 cggtgttgtg tggcgggtca gctccatcac cctcatgcag tagtgacctg acgcgctgtg
     6181 gcccgggccc tgctggttct gggccgcgag tggccccagc agacccgcca accccaccgt
     6241 tcgacgcccg ggtttgcttg ccgcatcgca tgtttcgcga cgtagatgtg ggtgtcgagg
     6301 cccccgtaaa cccaggtccc ctcaagcgtt gtgcacccat ctgttggccg agatgatcgt
     6361 agggcaaccc cgccgggggc ggggtcacaa cagaatggag tctccatgct gatctcccca
     6421 gctggaccac ggatcagggc gatgcgtcgc cggatcctgg ccgtcttggc cgtcatggca
     6481 ttggccgtcg tgggcctgca agcgctcccc gcccatgcgg cggcaccgcc accgatgaca
     6541 ccgtcgatca cctccaccgt gtcgaccatc gacgtcaacg gatgcggggc agcccgagga
     6601 ttcagccgtt acctcgtccc gaacctcggg ttcgaagagg actgcaacat gcacgacctc
     6661 tgttacggct caggcctgcc tcgcgcaggc tgcgacgacc gattcgccga gtcgatgagc
     6721 gacacctgcc agtcgaaccc gatctgcagg gccgctgccg cgctctacgc cgaggcggtc
     6781 cgaaaggccg gtgaaggcgc ctaccgcgaa gcgacccaac aacgcttgga cgagttcatc
     6841 aacaacatcg tcgcctgtgg agacgaccgg gcgtgcgagg aacgggtccg ccaagagcag
     6901 gccatggacg ccctcgtgaa cgaactcacc gcctgcaacg gaaacagggc ctgtgagcaa
     6961 gaggtgatcg accgtcgggt tcccaaggag gagccgaaga aggacgaccc gccggcagcc
     7021 acctctgacg aagcaccggc tgcggatgag ccggaggccg ccaaccccga aacccaggaa
     7081 ccggcgaccg agaacccacc gg
//
LOCUS       DAOMZY010000174         7092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_9936_length_7092_cov_7.460139, whole genome shotgun sequence.
ACCESSION   DAOMZY010000174 DAOMZY010000000
VERSION     DAOMZY010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7092
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9936_length_7092_cov_7.460139"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..1119)
                     /locus_tag="PLK64_10380"
     CDS             complement(28..1119)
                     /locus_tag="PLK64_10380"
                     /EC_number="5.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HPV80197.1"
                     /translation="MGSVRVAIVGVGNCASSLVQGVEYYRNADASASVPGLMHVQFGP
                     YHVSDVTFVAAFDVDDKKVGKDLSEAINASENNTIKICDVPTLGVEVQRGHTLDGLGK
                     YYRMTIDESAAEPVDIVATLKATKADVLVSYLPVGSENADKFYAQCAIDAGVAFVNAL
                     PVFIASDPEWAAKFEAAGVPIVGDDIKSQVGATITHRVMAKLFEDRGVTLDRTYQLNV
                     GGNMDFKNMLERERLESKKISKTQSVTSNLTGELAGKTSDRNVHIGPSDYVAWLDDRK
                     WAYVRLEGRAFGDVPLNLEYKLEVWDSPNSAGIIIDAIRAAKIAKDRGIGGALISASS
                     YLMKSPPVQRNDDLGRLKLEAFIAGTEER"
     gene            complement(1151..1732)
                     /locus_tag="PLK64_10385"
     CDS             complement(1151..1732)
                     /locus_tag="PLK64_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPV80198.1"
                     /translation="MSRRGDLLEFAVLGLLHESPMHGYELRKRLNTALGAFRALSYGS
                     LYPCLRELLGKGWIFESSSVEGDALSGSRRARIVYELTADGKERFQDMAETAGPTAWD
                     DEEFDVHFAFFARTKADVRLRILEGRRSRLEERLANIRTASVRNRERMDLYTAALQRH
                     GEESAEREVRWLEELISAERRGIATPNPHNTRA"
     gene            2059..4260
                     /locus_tag="PLK64_10390"
     CDS             2059..4260
                     /locus_tag="PLK64_10390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPV80199.1"
                     /translation="MSHDHTQPDPRATRSGRGNGAGTSAGTGSSGSKAKRSWWRAPRR
                     TWIKRVVWTVVGFLALGASAVAVAFATIQVPDVANGLAEAQTSIVYYDDGKTEMARIA
                     ELDREPLPLASIPKHLQHAVIAAEDRDFYTNAGISPTGIARSVWQAVSGSDVQGGGST
                     ITQQYVKNYFLTQDRTLDRKLREWVISIKIDRDMTKDQILEGYLNTIYFGRGAYGIKT
                     AARAYFGKDVSALTVQESAVLASVMNAPSLFDPALGAKQQTNLTKRVDYVLDGMVTMG
                     WLSAADRAPLTGLPTIAAKAPSRALSGPTGYIVAAVRRELTTKLQLSDDDIDRGGLRI
                     TTTISAPAQQAAQDAVAKDFPTTGDVKDVYAGLVAVRPGDGAIVAMYGGADYQARQFS
                     SATDAVIQGGSIFKAFGLIGALQQGISTKTQFDGNSPLRDPALGNTPVRNVGGRSYGT
                     VDLRKATASSVNTAFVRLNIKMGPSVTKTAAIAAGIPESTPGLGDDPTNVLGTASPHV
                     VDVANAYATIAAQGRRATPYLIKTVTSNAIDVTYQAVPNVVDAFSRDVAADVIDAMQQ
                     VTAAGGTGSRASAVGRPVAGKTGTSEEAKSVWFSGFTPQLSASVAMFKDVNGVPQPLT
                     DIGGLDGLTGGSYPLSIWIDFMKGALAGQDKVDFPKRVGIGDDKVHTAAPTPTAPATS
                     VPTSTPDPTNSPKPEPTPSPTNSSPTSIPPRPTNTGGPTNANKPTTPPGQG"
     gene            4325..5731
                     /locus_tag="PLK64_10395"
     CDS             4325..5731
                     /locus_tag="PLK64_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HPV80200.1"
                     /translation="MTAADRVVVPSREDPVARSASVVAGGPLGRYAVPLARGWRRLAA
                     GLVAASCLPMAASVALRGYCIDAGWNSPDQFFHMCYSDLPATFGDQNLGAGLGAFLAG
                     GVGAPTPVQPPLTALLLAFTGGLVPDGSAQARARFYFGIWAVLIALLLAVTVWFTAAA
                     VRRMPMRAAHVALSPVVALTAVVSPDLLGVALASGALWAWSRSRPVISGVLFGLAISA
                     RSYPVLLLVAIALVAVRAGRGRTVLPVAGAAIVTFAAVVALVALGNPGGAMRAYSTWV
                     GAEAGFGSPWVLGQLARPDSAQSMGFSLPEPLSPSALTLLAAAGWLVAILVGAWLAWS
                     GERRPGVAEVSLVMVAIVLVTGKSFPVQASIWLVPLVALVGIQWRDHLIWSGAEALHF
                     GAVWLYLAGSSVADRGLPSGWYLLLLLVRLLAIGWLVVQVWWQARDRMPHVVSPRETD
                     PLAGPLRDAPDALIVRVG"
     gene            5923..6210
                     /gene="rpsF"
                     /locus_tag="PLK64_10400"
     CDS             5923..6210
                     /gene="rpsF"
                     /locus_tag="PLK64_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPV80201.1"
                     /translation="MRHYELMVILDPELDDRTVAPSLDKLLAVVKKDGGSVDSVDIWG
                     RRRLAYDIKKKSEGIYAVVQMTAEPATALELDRQLNLNEVVMRTKLLRPGA"
     gene            6253..6852
                     /locus_tag="PLK64_10405"
     CDS             6253..6852
                     /locus_tag="PLK64_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPV80202.1"
                     /translation="MAGDTVITIVGNVTGDPELRFTPSGAAVANFTVASTPRAFDRQS
                     NEWKDGETLFMRCSVWRDAAENVAESLQRGTRVIVTGRLKSRSYETKEGEKRTVIEME
                     VDEVGPSLRYASAKVTKTQRGSGGGGGFGSSGTGQQGGYAADTDPWATGPSGGGQGGS
                     GGYGGQGGAGAPSGGPGQAPQGGNQGGWGSAPSYDEPPF"
     gene            6947..>7092
                     /locus_tag="PLK64_10410"
     CDS             6947..>7092
                     /locus_tag="PLK64_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPV80203.1"
                     /translation="MPKPPPRIKPGKKKANPLKAAKIVSIDYKDAALLRKFISDRGKI
                     RARRV"
BASE COUNT         1085 a         2478 c         2376 g         1153 t
ORIGIN      
        1 cgggctcgta cctcgcccgc tagatcgtca gcgctcttcg gtgccggcga tgaaggcctc
       61 gagcttcaac cgacccaggt cgtcgttgcg ctgcacgggc ggcgacttca tcaggtagga
      121 cgacgccgag atcagcgcgc cgccgatgcc gcggtccttg gcgatcttcg ccgcacggat
      181 ggcgtcgatg atgatgccgg ccgagttggg ggagtcccac acctcgagct tgtactccag
      241 gttcagcggt acgtcaccga acgcgcggcc ctcgaggcgc acataggccc acttgcggtc
      301 atcgagccag gcgacgtagt ccgacgggcc gatgtgcacg ttgcggtcgg aggtcttgcc
      361 ggccagctca ccggtgaggt tggacgtcac cgactgggtc ttggagatct tcttggactc
      421 cagacgctca cgctcgagca tgttcttgaa gtccatgttg ccgccgacgt tgagctggta
      481 ggtgcggtcc agcgtgacgc cgcggtcctc gaacagcttg gccatgacgc ggtgggtgat
      541 ggtcgcgccg acctggctct tgatgtcgtc gccgacgatc gggacaccgg ctgcttcgaa
      601 cttggccgcc cactccgggt cgctggcgat gaagaccggc agtgcgttga cgaaggccac
      661 accggcgtcg atcgcgcact gggcgtagaa cttgtcagcg ttctccgagc ccaccggcag
      721 gtaggacacg agcacgtcgg ccttggtcgc cttgagcgtg gcgacgatgt cgaccggctc
      781 ggccgccgac tcgtcgatgg tcatccggta gtacttgccg aggccgtcga gggtgtgccc
      841 gcgctgcacc tcgacaccca gggtcgggac gtcgcagatc ttgatggtgt tgttctccga
      901 cgcgttgatc gcctcggaca ggtccttgcc gaccttcttg tcgtcgacgt cgaacgcggc
      961 cacgaaggtc acgtcactca cgtggtaagg cccgaactgg acgtgcatga gacccggcac
     1021 cgaggccgag gcgtcagcat tgcggtagta ctccacgccc tgcacgagcg agctcgcgca
     1081 gttgccgacg ccaacgatgg cgacgcgcac agatcccatg agggttctcc ttgtgtgatg
     1141 gcccggccgg tcaggcgcgg gtgttgtgag ggttgggggt ggcaatcccc cgacgttcag
     1201 ccgagatcag ttcttcgagc cagcggactt cgcgttccgc cgactcttcg ccgtgccgtt
     1261 gcagcgcagc ggtatacagg tccatccgct cccggttacg caccgaggct gtgcggatgt
     1321 tggccaggcg ctcctccagc cgcgaacggc gcccctcgag gatgcgcagt cgcacatcgg
     1381 ccttggtccg ggcgaagaag gcgaagtgga cgtcgaactc ctcgtcgtcc cacgccgtcg
     1441 gcccggccgt ctcggccatg tcctggaagc gttccttgcc gtcggccgtc agctcgtaga
     1501 cgatccgcgc ccgccggctg cccgagagcg catcgccctc gacactcgac gactcgaaga
     1561 tccagccctt gccgagcagc tcgcgcagac acggatatag cgacccatag gacaacgccc
     1621 ggaacgcacc cagagccgtg ttgagtcgct tgcgcagctc gtagccgtgc atcgggctct
     1681 catggagcaa cccgaggacg gcgaactcca gcagatcacc acgtcgagac accgtcactc
     1741 acctccgcct ctggtccggt ccgttgatcc ggtatgccgt gtgcctgggt ccgcgtcgga
     1801 cccgggctgt cgttcgtcgc accttcggat gggtacgcgc gtgcgtcgat gaccagcaga
     1861 ctctagcacc agatatatcg aaccgataca tcatcccgat gtgtcgcgtg tcgcggcggg
     1921 gtgccgacgg gcagccggtg gccagtttgg gtgtgtgcga ttcggtagct cacagcccgt
     1981 ccgctcatac tggatctcct gacggggaat cgaccggaac cctccaacgg gcgacgacgg
     2041 caggaaaggc actctcgcgt gagccacgat cacacccagc cggacccgcg ggccacccgc
     2101 tccggcaggg gcaacggcgc aggcaccagc gcaggcaccg gcagctccgg cagcaaggcc
     2161 aagcgcagtt ggtggcgcgc cccccgacgg acctggatca agcgggtcgt ctggaccgtc
     2221 gtcggcttcc tcgccctcgg cgccagcgcg gtcgctgtgg ccttcgccac catccaggtg
     2281 cccgatgtcg ctaacgggct ggccgaggcg cagacctcga tcgtctacta cgacgacggc
     2341 aagaccgaga tggcccggat tgccgagctc gaccgcgagc ctcttcctct ggcgagcatt
     2401 cccaagcatc tgcagcacgc ggtcatcgcc gccgaggacc gcgacttcta caccaacgcg
     2461 gggatctcac ccaccggcat cgcccgctcc gtctggcagg ccgtcagcgg cagcgacgtc
     2521 cagggtggcg ggtcgacgat cacccagcaa tacgtgaaga actacttcct cacccaggac
     2581 cgcaccctcg accgcaagct tcgcgagtgg gtcatctcga tcaagatcga ccgggatatg
     2641 accaaggacc agatcctcga gggctacctc aacaccatct acttcgggcg cggcgcctat
     2701 ggcatcaaga cggccgcccg ggcctacttc ggcaaggacg tcagcgcgct cacggtccag
     2761 gagtcggccg tcctggccag cgtcatgaac gccccgagcc tcttcgaccc ggccctcggt
     2821 gccaagcagc agaccaacct caccaagcgg gtcgactacg tcctggacgg catggtgacg
     2881 atggggtggc tctcggccgc cgaccgcgcg ccgctcaccg gcctgccgac gatcgccgcc
     2941 aaggccccgt cgcgggccct ctccggtccg accggctaca tcgtcgccgc ggtccgccgc
     3001 gagctgacca ccaagctgca actgtccgac gatgacatcg accgcggcgg cctgcggatc
     3061 acgacgacga tcagtgcccc cgcgcagcag gccgcgcagg acgccgtcgc caaagacttc
     3121 cccacgaccg gggacgtcaa ggatgtgtat gccgggctgg tcgccgtccg ccctggcgac
     3181 ggcgccatcg tcgcgatgta tggcggtgcc gattaccagg cccgtcagtt ctcctcggcg
     3241 accgacgcgg tgatccaggg cggctcgatc ttcaaggcgt tcgggctcat cggcgccctg
     3301 cagcagggca tctccaccaa gacccagttc gacggcaaca gcccgctgcg cgaccccgcc
     3361 ctaggcaaca caccggtccg caacgtcggc ggccggtcct acggcaccgt cgacctgcgc
     3421 aaggcaaccg cctcgtcggt caacacggcg ttcgttcgac tcaacatcaa gatggggccg
     3481 tcggtgacga agacggcggc gatcgcggcc ggcataccgg agagcactcc ggggctggga
     3541 gacgacccga ccaacgtcct tgggacggcc tcgccgcacg tggtcgacgt cgccaacgcc
     3601 tacgccacga tcgcggcgca aggccgccgg gcgacgccct acctcatcaa gacggtgacc
     3661 tccaacgcca tcgacgtcac ctaccaggcg gtccccaacg tcgtcgacgc gttctcccgc
     3721 gacgtcgccg ccgacgtcat cgacgccatg cagcaggtga ccgcggccgg cggcacgggg
     3781 tcgcgcgcga gcgcggtcgg ccgtccggtg gcgggcaaga ccggcacctc cgaggaggcg
     3841 aagtcggtgt ggttcagcgg cttcaccccc cagctgtcgg catcggtcgc gatgttcaag
     3901 gacgtcaacg gggtgcccca accgctcacc gacatcggcg gtctcgacgg gctgaccggc
     3961 ggctcctacc ccttgtcgat ctggatcgac ttcatgaagg gcgcgctggc gggtcaggac
     4021 aaggtcgact tccccaagcg cgtcggcatc ggcgacgaca aggtccacac ggccgcgccg
     4081 accccgacgg cccctgcgac gtcggtgccc acgagcacgc ccgaccccac caactcgccg
     4141 aaacccgagc ccaccccgtc gccgaccaat tcgtcgccga cgagcatccc gccgcggccg
     4201 accaacaccg gcggcccgac caacgccaac aaaccgacaa cgccacccgg tcaggggtag
     4261 cgccccgcat cgacaaaccg gcgcgcaggc ggcatacctc aggcgggcgg cataccctgt
     4321 gggcgtgaca gctgcggacc gcgtcgtcgt cccctcccgg gaggaccccg ttgcgcgctc
     4381 ggcgagcgtc gtggcggggg gaccgcttgg ccggtatgcc gtcccgctgg ctcgcgggtg
     4441 gcgccgcctg gccgccggat tggtggccgc gtcgtgcctg ccgatggcgg cgtcggtggc
     4501 gctgcgcggc tactgcatcg atgcggggtg gaacagcccc gaccagttct tccacatgtg
     4561 ttactccgac cttccggcga ccttcggcga ccagaacctc ggcgccggcc tcggggcctt
     4621 cctcgctggc ggcgtgggcg cgccgactcc ggtgcagccg ccgctgaccg cgctgctgct
     4681 ggccttcacc ggcggcctcg tgcccgacgg gtcggcgcag gctcgggccc ggttctattt
     4741 cggcatctgg gcggtgctca tcgccctact cctcgccgtc accgtctggt tcacggcggc
     4801 agcggtccgg cgtatgccga tgcgcgcggc tcacgtcgct ctgtcaccgg tcgtcgcgct
     4861 caccgcggtc gtctccccgg acctgctcgg tgttgccctc gcgtcggggg ccctgtgggc
     4921 ctggtcgcgc tcgcggccgg tgatcagcgg agtgctgttc ggcctggcga tctcggcgcg
     4981 ttcctatccc gtcctgttgc tcgtggcgat cgcgctggtc gcggtgcggg cggggcgggg
     5041 gcggaccgtc ctgccggtcg cgggagcagc catcgtcacc ttcgcggcgg tggtggcgtt
     5101 ggtcgccctg ggcaatcccg gcggagcgat gcgggcctat tcgacgtggg tcggggccga
     5161 ggcggggttc ggctcgccgt gggttctcgg gcagttggcc cggcccgaca gcgctcagtc
     5221 gatgggtttc agcctgcccg aaccgttgtc cccgagcgcc ctcacgctgt tggcggccgc
     5281 cgggtggctc gtcgcaatcc ttgtcggggc ctggttggcc tggagcggtg agcgtcggcc
     5341 aggggtcgcc gaggtgtcgc tcgtcatggt ggcgatcgtc ctcgtgacgg ggaagtcctt
     5401 ccccgtgcag gcgtcgatct ggctcgtccc tctcgtcgcc ctcgtcggga tccagtggcg
     5461 cgaccacctc atctggtccg gggccgaggc cctgcatttc ggggcggtct ggctctatct
     5521 cgctgggagt tcggtggccg atcgcgggct gccctcgggg tggtacctgc tgctcctgct
     5581 ggtgcgtctg ttggcgatcg gatggctcgt ggtgcaggtc tggtggcagg cccgcgaccg
     5641 gatgccgcac gtggtgtcgc cccgggagac cgatccgttg gccgggccgc tgcgcgacgc
     5701 cccagacgcc ttgatcgtcc gcgtcggctg aaacgagcgc gattaggtcc ccctgcgggg
     5761 gtgccgtaac ctaggacggt tcgcctgcgg catacccctg tggaaaactt ccggccccct
     5821 gtggcgcggt gaggcaggat cggtggtcga ggcgcggaca catgcaaccc tcctgtcacg
     5881 gagagaccgt gaccgcgaag accagaggag gtgggtcaac gcatgcgtca ttacgaactc
     5941 atggtcatcc tggaccccga gctcgacgac cgcacggtcg ccccgtcgtt ggacaagctg
     6001 cttgcagttg tcaagaagga cggcggcagt gtcgactctg tcgacatctg gggtcgtcgg
     6061 cgcctcgcct acgacatcaa gaagaagtcc gagggcatct acgccgtcgt tcagatgact
     6121 gccgagccgg cgacggctct tgagctggac cgccagctca acctcaacga ggtggtcatg
     6181 cggaccaagc tcctgcgccc cggcgcctga gctcggcagc gcccttctct agcaagcaag
     6241 gagaggtagc acatggctgg cgacaccgtc atcaccatcg tcgggaacgt cacgggtgac
     6301 cccgagttgc ggttcacccc ttccggcgcc gccgtcgcga acttcaccgt cgcctcgacg
     6361 ccgcgcgctt tcgaccgcca gagcaacgag tggaaggacg gggaaaccct gttcatgcgc
     6421 tgctcggtgt ggcgggacgc ggccgagaac gtcgccgagt cgctgcagcg tggcacccgg
     6481 gtcatcgtca cgggccggct caagagccgt tcctacgaga ccaaggaagg cgagaagcgc
     6541 accgtcatcg agatggaggt cgacgaggtc ggcccgtcgc tgcgctacgc ctcggccaag
     6601 gtcaccaaga cccagcgcgg gtccggcggc gggggcggct tcggctcctc cggcacgggc
     6661 cagcagggcg ggtatgccgc tgacaccgac ccgtgggcga ccggcccgtc gggtgggggt
     6721 cagggcggtt ccggtggcta cggcggccag ggtggtgccg gtgctccgtc cggtggtccc
     6781 ggccaggctc cgcagggtgg caaccagggt ggctggggct cagcgcccag ctacgacgag
     6841 ccgccgttct gatcgggtgt ttcgcgacct gacccttgtg gttggcggcg cgaaacccca
     6901 tctctcacag actttttcga catagctcac aaggagaacc acagaaatgc ccaagccccc
     6961 gccgcggatc aagcccggga agaagaaggc caacccgctc aaggccgcca agatcgtcag
     7021 catcgactac aaggatgctg cgctgctgcg caagttcatc tccgaccgcg gcaagatccg
     7081 cgctcgccgg gt
//
LOCUS       DAOMZY010000175         7081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_9957_length_7081_cov_4.890122, whole genome shotgun sequence.
ACCESSION   DAOMZY010000175 DAOMZY010000000
VERSION     DAOMZY010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7081
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9957_length_7081_cov_4.890122"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="PLK64_10415"
     CDS             <1..866
                     /locus_tag="PLK64_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPV80204.1"
                     /translation="NKGVNPDEVVAIGASLQAGVLKGERKDVLLIDVTPLSLGIETEG
                     GRFTKLIERNTAIPTKRSEIFTTAADNQPSVLIQVFQGEREIAQHNKPLGTFELTGIA
                     PAPRGIPKIEVTFDIDANGIVHVSAKDMGTGKEQSMTISGGSALPKEEIERMVREAEQ
                     HAEEDRQRVEEIETRNQAEQVVYSTEKFLADNADKIPADVKGEVETALADVKTVIAKD
                     STASVEDIKAKVATLDEVRSKMGQAMYAAAAASGEGDGSADAGPQDAESAAGPQDDVV
                     DAEVVEDEDKK"
     gene            863..1540
                     /gene="grpE"
                     /locus_tag="PLK64_10420"
     CDS             863..1540
                     /gene="grpE"
                     /locus_tag="PLK64_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPV80205.1"
                     /translation="MTHGHEHDAEGRGHHGPAHPGDSGSAEPGGFDEFDHPDFADIAA
                     AAAASLREEHPEGTGIPVVSDGTAAQGDGAEPSLAPLTGDAALAAERLEDLRRLQAEY
                     VNYKKRVDRDRSVARDQGISHVLESMLPVLDEIHLAREHGDLGEGPAATIVDKLEAVL
                     AKLGIERFGAAGDEFDPNHHEALMRMEADLPEGATTTTVVQVLQPGYRLGERIVRAAR
                     VAVADPQ"
     gene            1577..2635
                     /locus_tag="PLK64_10425"
     CDS             1577..2635
                     /locus_tag="PLK64_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HPV80206.1"
                     /translation="MASQDWLSKDFYAVLGVPKEADDAAIKKAYRKLARKLHPDQNAG
                     DPKAEERFKEIGEAYAVLSDPEERHQYDQLRAMTTGGARFTAGPGGAGGGFEDVFSGM
                     FGNGGNMRFNTGGRGGAGGAGPTPDLQDLLGGLFGGAGQGGAGGFGGDPYGGFGGDPY
                     GGYGAPRGPRRGADVSARTTLDFRQAVQGDTVTLATGDGDRLTTRIPAGVKDGQKIRL
                     RGKGQPGGPGAERGDLILTVAVTPHPVFGRDGDNLTVDLPVTFAEAALGATVSVPTLS
                     GTAVRVKVAPGTPSGRVLRIKGRGVKHAKGDGDLLAKVQVIVPQRLTDEAREALERLR
                     DQEEGLDPRAELFAKAAH"
     gene            2671..3141
                     /locus_tag="PLK64_10430"
     CDS             2671..3141
                     /locus_tag="PLK64_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPV80207.1"
                     /translation="MLSDQRAGGRRAAYQPSDDSPVFVISVAAQLAGMHSQTLRQYDR
                     LGLVTPSRTRGGGRRYSTRDVALLREVQRLSQEEGISLAGIQRILRLEHEVAALRARV
                     SELTDELGAARHTVGHRVFAAGPAGDIVAMRPGQRPSRPAGPSRALVVWRPQRG"
     gene            3299..4489
                     /locus_tag="PLK64_10435"
     CDS             3299..4489
                     /locus_tag="PLK64_10435"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPV80208.1"
                     /translation="MTWLGVVASAALNYRRGSVPLIWWVLSVIVLVTFLALGGSTPRT
                     STRDRVLFTILTLSAVAACGVWGADLMSGVLLVLVAGVAGWVVPPKVSIPLMLAMTAI
                     LAFFMWTAKSTVVWALLYGSLLFFASLMVSVVVREQAARRIAATVAAELEVANARLLG
                     ANDALRDAHSRLAEASKAEERLRISRDLHDGMGNQLTALSLQLDLIGRLVDGAAVTHL
                     DQAKALTTELIRDTRGVVSQLRDTRVSPRDKIARLARSMPRPQTSLDLTEALDEVPEP
                     VAETVVRLVQEGLTNAARHADAEHAWVVVSCAPDGLRLQVRDNGRGAPTLVPGNGLSG
                     MAERVALLGGDLTWSSPSSGGFIVTASIPVTQASPDTPVEAAGPDVPDTHNTGSPMSD
                     SVVG"
     gene            4738..5409
                     /locus_tag="PLK64_10440"
     CDS             4738..5409
                     /locus_tag="PLK64_10440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPV80209.1"
                     /translation="MSEGLRLGVVDDHPAILRGILTILVELCPEITETFTTSTVPALL
                     GAHRSLDIVVLDVRLADGSDPAANVAAVVDAGLPVLLYTQEQRRGVLARCFRAGALGV
                     VDKSEEPGVLAAAIRVVAAGEPYLSSGWAAAVEDADDWPVPELAPRESEALGLYATGL
                     PLKSVARRMHLSEDTVKEYLDRVRRKYTRAGRPAYTKVDLYQRAVEDGYLPPPGEGAP
                     ADPAR"
     gene            5406..>7081
                     /locus_tag="PLK64_10445"
     CDS             5406..>7081
                     /locus_tag="PLK64_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80210.1"
                     /translation="MILTHEVLGRWLHRGACALTGVFVVAVPGLQFIDPVAYPPLLFE
                     VWLVLALATLGWALWDAYRCVRVPWGLRACVVVALAAGLTQMVGGWRNSDSLTIPPLL
                     QAMAPAFGVVGFALSPRAAVPIGLICGTAFAVSEWGVDPGLPAVASGLVVLGSGLLAG
                     GAIDLLYRAAAKVEQALDERWDAREAIARSAVRAEQTQLWDGLVHDKVLGALRLAARA
                     RDDSDRRAAGELAREALITGSAPTPLGGDGSGRALRSGVERVCHRLGLQLRWTVEERE
                     GSGPTDGAAAAVLAAAEEALVNVSRHSGSQAVTIAGSVGGDLRLDIIDAGPGFDLLAP
                     RPGRLGIDRGIIGRLARVGGSAQVLTRPGHGTTVRLVVPAAGRDLRPQLPPPLETWRQ
                     QDFVGLFLMGVALTGVYAAAAIATADRTKSGGVVAICLALVVALGLTASLAPGHRDDV
                     AALIVVGVGVVCALGMWNLRRPFDEGWATWFVGALNATVVVLTGRFRTRWGFLAVASA
                     ALGLAVGQVLAAGVVHLGVITALVPQQIAFVCAIWGIRRALDLATSAINEA"
BASE COUNT         1030 a         2401 c         2529 g         1121 t
ORIGIN      
        1 ccaacaaggg cgtcaacccg gacgaggtcg tcgccatcgg tgccagcctg caggccggtg
       61 tcctcaaggg tgagcgcaag gacgtcctgc tgatcgacgt cacgccgctg tccctgggca
      121 tcgagaccga gggtggccgg ttcaccaagc tcatcgagcg caacacggcg atcccgacca
      181 agcggtccga gatcttcacg accgcggccg acaaccagcc gtcggtgctc atccaggtct
      241 tccagggcga gcgcgagatc gcgcagcaca acaagcctct gggcaccttc gagctcaccg
      301 gcatcgctcc ggctccccgt ggcatcccga agatcgaggt caccttcgac atcgacgcca
      361 acggcatcgt gcacgtgtcc gccaaggaca tgggcaccgg caaggagcag tcgatgacga
      421 tctccggcgg cagcgcgctg cccaaggagg agatcgagcg gatggttcgc gaggccgagc
      481 agcacgccga ggaggaccgc cagcgcgtcg aggagatcga gacccgcaac caggccgagc
      541 aggtcgtcta ctccaccgag aagttcctcg ccgacaacgc cgacaagatc ccggccgacg
      601 tcaagggtga ggtcgagacc gcgctcgccg acgtcaagac ggtgatcgcc aaggactcga
      661 ccgcttccgt cgaggacatc aaggcgaagg tcgcgacgct cgacgaggtg cgctccaaga
      721 tggggcaggc catgtatgcc gcggccgcgg cttccgggga gggcgacggt tccgccgacg
      781 ccggcccgca ggacgccgag tccgctgccg gtccgcagga cgacgtggtt gacgccgagg
      841 tcgtcgagga cgaggacaag aagtgaccca cgggcacgag cacgacgcgg aggggcgggg
      901 ccatcacggc cccgcccatc cgggcgactc gggctcggcc gagcccggcg gcttcgacga
      961 gttcgaccac ccggacttcg ccgacatcgc ggcggctgcg gccgccagcc tgcgggagga
     1021 gcaccccgag gggaccggca tacctgtggt ctctgacgga accgccgccc aaggtgacgg
     1081 tgccgagccg tccctcgcgc ccttgacggg agacgcagcc ctcgcggccg agcgccttga
     1141 ggacctgcgt cggttgcagg ccgaatacgt caactacaag aagcgcgtcg accgggaccg
     1201 gtccgttgcg cgcgaccagg gcatcagcca cgtcctcgag tcgatgctgc cggtgctcga
     1261 cgagatccat ctcgcccgcg agcatggcga tctcggcgag gggcccgcgg cgacgatcgt
     1321 cgacaagctt gaggcggtgc tggccaagct gggcatcgag cggttcgggg cggcggggga
     1381 cgagttcgac cccaaccacc acgaggcact catgcggatg gaggccgacc tgccggaggg
     1441 cgccacgacc accacggtcg tccaggtgct ccaacccggt tatcgactgg gcgaacggat
     1501 cgttcgcgcg gcccgcgttg cggtcgcgga tccccaataa caccaacagt tttcattgag
     1561 aggagggcgc gtcgaattgg ctagtcagga ttggttgtcg aaggacttct acgcagtgct
     1621 cggcgtgccc aaagaggccg acgacgccgc catcaagaag gcctaccgca agctcgcccg
     1681 caagctgcac cccgaccaga acgccggtga ccccaaggcg gaggagcgct tcaaggagat
     1741 cggcgaggcg tatgccgtgc tgtccgatcc cgaggagcgt caccagtacg accagttgcg
     1801 ggcgatgacc accggcggtg ctcgattcac ggccgggcca ggtggcgccg gcggtggctt
     1861 tgaggatgtc ttctccggga tgttcggcaa cggcggaaac atgcggttca acacgggtgg
     1921 gcggggtggt gccggtggcg ccggtccgac gcccgaccta caggacctcc tcggcggcct
     1981 cttcggcggg gccggacagg gtggcgccgg cggcttcggc ggtgacccct acgggggctt
     2041 cggcggtgac ccctacggcg gctacggcgc ccctcggggt ccgcgacgcg gcgccgacgt
     2101 gtcggcacgc acgacgctcg acttccgtca ggccgtccag ggcgacacgg tgacgctggc
     2161 aacgggtgac ggtgatcggc tcaccacacg gatcccggcc ggggtcaagg acggtcagaa
     2221 gatcaggctg cgcggcaagg gtcagccggg gggacccggc gctgagcggg gcgatctcat
     2281 cctcaccgtc gccgtcaccc cgcacccggt gttcgggcgc gacggtgaca acctcacggt
     2341 cgacctgccg gtgaccttcg ccgaggcggc gctcggtgcc accgtgtcgg tcccgaccct
     2401 cagcggaacc gcggtgcggg tcaaggtcgc gccgggcacg cccagtggcc gggtgctgcg
     2461 catcaagggt cgtggggtca agcacgccaa gggtgacggt gatctgctgg ccaaggtcca
     2521 ggtgatcgtc ccgcaacgcc tcaccgacga ggcccgcgag gccctcgagc ggcttcgtga
     2581 ccaggaggag ggcctcgacc cccgggccga gctcttcgcc aaggccgccc actgaggccc
     2641 ggaaggtgac ccccatggtc ggtgaccagc atgttgagtg accagcgcgc gggaggtcgg
     2701 cgggcggcat accaaccgtc cgacgactct cccgtgttcg tcatctcggt cgccgcgcaa
     2761 ctggccggga tgcactcgca gaccctgcgc cagtacgacc ggctcgggct cgtgacgccg
     2821 tcgcggacaa ggggtggtgg gcggcgttac tccacgcgcg acgtcgcgct cctgcgcgag
     2881 gtgcagcggc tctcgcagga agagggcatc agcctggccg gcatccagcg catcctgcgc
     2941 ctcgagcatg aggtcgcggc gctgcgggcg cgagtgagcg agctgaccga cgaactcggc
     3001 gcggcgcggc ataccgtcgg gcaccgggtg ttcgcagcgg gaccggcggg cgacatcgtc
     3061 gcgatgcgtc cgggtcagcg accgtcgcgc cctgcggggc cgtcgcgggc gctggtcgtt
     3121 tggcgacccc agcgcggctg accgtgacgg cctgcaaagg cacccgcgaa taggcctgag
     3181 tgctctggta attccccctg ccgcctccta ctctggccct gtggcgggcg gtgaatggcg
     3241 ggcggtcccg tggcgaaaga acgattttgt cgcttggggc tccgcactcg gtgggacggt
     3301 gacctggctt ggggtggtgg ccagcgccgc cctgaattac cgtcggggtt ccgtcccgct
     3361 gatctggtgg gtgctcagcg tcatcgtcct agtgaccttc ttggccctcg gtggttcgac
     3421 ccctcggacc tcgacccgcg atcgggtgct cttcacgatc ctcaccctgt ccgccgtcgc
     3481 cgcctgcggg gtgtggggcg ccgacctgat gtcgggggtc ctgcttgtcc tggtcgccgg
     3541 cgtagccggt tgggtcgtcc cgcccaaggt gtcgatcccg ctcatgctcg cgatgacggc
     3601 gatcctcgcc ttcttcatgt ggacggcgaa gtccacggtg gtgtgggcgc tgctctacgg
     3661 aagcctgctg ttcttcgcct cgctcatggt ctcggtggtc gtccgcgagc aagctgcccg
     3721 gcggatcgcc gccaccgtcg ctgcggaact cgaggtggcc aacgcgcgcc tgctcggcgc
     3781 caacgatgcc ttgcgcgatg cccacagcag gctggccgag gcgagcaagg ctgaggagcg
     3841 cctgcgcatc tcacgcgacc tgcacgacgg catgggcaac caattgactg cgctgtcact
     3901 gcaactggac ctgatcggac ggctcgtcga cggcgccgcg gtcacccacc tcgaccaggc
     3961 caaggcactg accaccgagt tgatccgcga tacgcgggga gtcgtcagcc agttgcgcga
     4021 cacgcgggtg tcccctcgcg acaaaatcgc tcgcctggcc cgctcgatgc cccggcccca
     4081 gacctcgctc gatctgaccg aagccctcga tgaggtcccc gagccagtgg ccgagaccgt
     4141 cgtcaggctg gtccaggagg ggttgaccaa cgccgccagg cacgcggatg ctgagcatgc
     4201 ctgggtcgtg gtcagctgtg cgcccgacgg gctgcgtttg caggtccgcg acaacggcag
     4261 gggcgctccc actctggtcc cggggaacgg cctgtccggg atggctgagc gggtcgcact
     4321 gctcggcggt gacctgacgt ggtcaagccc gtcgagcggc gggttcatcg tgacggcctc
     4381 gatcccggtg acccaggcgt ccccggacac accggtcgaa gcggccggcc cggatgtccc
     4441 cgacactcac aacactgggt cgccgatgtc cgacagcgtg gtcggctgag tccaggcagc
     4501 gagcctgcga gtcggagctc cctcgacgtg gcagagggta tgccgcccga cttgccccag
     4561 cggggcggca taccctccga ccgtcacgtc aactcgagac tcgagcgcct ccgcaggctc
     4621 gaatcccccc tgcgacgcaa taccgagcct gctccaatct ccgcgctgga gtaagtcccc
     4681 cgtttcaggg acattcccac gtgcgatgag aattgtcgcg aatggcagac tcgcagtatg
     4741 agcgaggggc tgcggctcgg tgtcgttgac gatcacccgg cgattttgcg cggaatcctc
     4801 acgatccttg ttgagttgtg cccggagatc accgagacgt tcacgacctc gacggtcccc
     4861 gcgctcctcg gggctcatcg cagcctcgac atcgtggtcc tcgacgtgcg cctggccgac
     4921 ggcagcgacc cggctgccaa cgtggcggcg gtggtcgacg ccggccttcc ggtgctgctc
     4981 tacacgcagg agcaacgccg cggtgtgctg gcccggtgct tccgagccgg ggccctgggc
     5041 gtggtcgaca agagcgagga gccgggcgtc ctcgcggcgg cgatccgcgt cgtcgctgca
     5101 ggagagccct acctcagctc gggctgggcc gcagcggtgg aggacgccga cgattggcca
     5161 gtccccgagc tggctccgcg cgagagcgag gccttggggc tctacgccac gggccttccg
     5221 ctcaagtccg tcgctcggcg catgcacctg tcggaggaca cggtcaagga gtacctcgat
     5281 cgggtgcggc gcaaatacac gcgagccggc cgaccggcat acacgaaggt ggacctctat
     5341 cagcgggcgg tggaggacgg ctacctgccg cccccggggg agggcgcgcc cgccgacccg
     5401 gcccgatgat cctgacccat gaggtcctcg gccggtggtt gcatcgcggc gcgtgcgctc
     5461 tgacgggtgt cttcgtcgtc gcggtcccgg gtctgcagtt catcgatccg gtggcgtatc
     5521 cgccgctgct cttcgaagtg tggctggtgt tggccctcgc aacgctcggc tgggccttgt
     5581 gggacgcgta ccgctgcgtt cgcgtccctt gggggctgcg cgcctgcgtt gttgtggccc
     5641 tcgctgcggg gctgacgcag atggtggggg gctggcgcaa cagcgactcg ttgaccatcc
     5701 ctccgctgct gcaagccatg gcccccgcct tcggtgtcgt ggggtttgcg ctgtcccccc
     5761 gcgcggcggt tcccatcggg ctgatctgcg gcacggcgtt cgcggtcagc gagtggggcg
     5821 tcgatccggg gctgcccgcc gtggcttccg gcctggtcgt cctcgggtcg gggctgctcg
     5881 cgggtggggc catcgatctg ctctaccgag ccgccgccaa ggtcgagcaa gccttggacg
     5941 agcgctggga tgcccgcgaa gcgatcgccc gctctgcagt cagggccgag cagacccaac
     6001 tgtgggatgg actcgttcac gacaaggtgt tgggtgccct gcggctggca gcccgggccc
     6061 gggacgacag tgaccgacgg gcagccgggg agttggcgcg cgaggcgctc atcacgggga
     6121 gcgccccgac acccctcggc ggcgacggct ccgggcgcgc cttgcgatct ggcgttgagc
     6181 gagtctgtca tcggctcgga cttcagctgc ggtggacggt ggaggaacgc gaaggctcgg
     6241 ggccgaccga tggtgccgct gcagcggtcc tggccgctgc tgaagaggcg ctggtcaacg
     6301 tgtcccgaca ctccggatcc caggccgtta ccatcgcagg gtcggtcggc ggtgacctgc
     6361 gcctcgacat catcgacgcc gggccggggt tcgacctgct ggccccgcgc ccgggtcgcc
     6421 tgggcatcga ccgcggcatc atcggacggc tggcccgggt cggcgggtcc gctcaggtcc
     6481 tcacccgtcc cgggcatggc acgacggtcc ggttggtggt gcccgctgct ggtcgtgatc
     6541 tccggcctca actgccgcca ccgctcgaga cgtggcggca gcaggacttc gtcgggctct
     6601 tcctcatggg agttgccctc accggggtct atgccgctgc cgccatcgcc acggccgacc
     6661 ggacgaagtc gggaggtgtt gtcgcgatct gccttgcctt ggtcgtggcg ctggggttga
     6721 cggcctcgtt ggccccgggt catcgggacg acgtggccgc actgatcgtc gtcggtgttg
     6781 gagtggtctg cgcactgggg atgtggaatc tgcgcagacc gtttgacgag gggtgggcca
     6841 cctggttcgt cggggcgctc aacgccaccg tcgtcgtgct gaccgggcgg tttcggaccc
     6901 ggtgggggtt cctcgccgtc gctagtgctg cgctcggcct tgcggtgggc caggttctgg
     6961 cggccggtgt cgtccacctc ggcgtcatca cggctctcgt gccgcaacag attgccttcg
     7021 tgtgcgccat ctgggggatc cgccgggctc tcgacctcgc cacgagcgcg atcaatgagg
     7081 c
//
LOCUS       DAOMZY010000176         7071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_9987_length_7071_cov_4.219783, whole genome shotgun sequence.
ACCESSION   DAOMZY010000176 DAOMZY010000000
VERSION     DAOMZY010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7071
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9987_length_7071_cov_4.219783"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2659
                     /locus_tag="PLK64_10450"
     CDS             <1..2659
                     /locus_tag="PLK64_10450"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HPV80211.1"
                     /translation="QALAPLRDITPSLEDAGLPRSARLLECVGLTDPTPDAVVAGWGQ
                     PQTDVVIGAGFDGMFRIDLRRDGPHALVAGTTGSGKSELLQTIVASLALANRPDQLTF
                     VLVDYKGGSAFKECNKLPHTVGMVTDLDTHLVGRALTSLGAELRRREHLLSVPGAKDL
                     EDYWALCRNDPTLPTIPRLVLVIDEFASLKAELPDFVTGLVTIAQRGRSLGIHLILAT
                     QRPSGVVSADIRANTNLRIALRVTDESESRDVIDAPEAGRILASTPGRGYARLGPSGV
                     LPFQAGRVGGRRPDAPSETRTIPTPLAWGIPWSRTGIPAPGRPTTAASDTDEGETDLS
                     VLVTAVRGAVDHLGMPPQHSPWLPALPGFVTLDELRARLVERDPAAAADPFTVGWALE
                     DRPEDQAQVPATFTLGTSGHLFLVGGPRSGRSTALRTLVGALAEKVAARDLHVYGLDC
                     GNGALLPLTVLAHTGAVVRRTEVERASRLIRRLTEEVARRQSVLGAHGFADIQELRSA
                     AAPDDRLPYVLLVLDRWEGFMSDLADVDVGSLLDGMVTLLREGASVGIQVVVTGDRTL
                     NSARVASLVEAKLALRLPDRQDYASLGLKVRELPEEFPEGRGVWGERSIEAQIAVLGA
                     DASGQGQGTSLRELAAATAERDRDLPRALRPMALGALPDQVAWADVAADFDPGARPPS
                     WLPFGIGGDLVEPIGLDLDRSPVALVAGESRTGKTATLCFLAAYARRSGRAVLGFVPR
                     DNDLSADLGEGACIVGQEDPNAAVARLRDLPEGALVLVDDVEAYKDAPLTPLMNALVR
                     QAADRGFTVVLSGRSADLGAGYSGWMFEARRGRHGLVLSPSEAIDGELFGSRVARTAL
                     RSRPHAGRGVLFDSTGAQTTVQVPLVERG"
     gene            complement(2719..3018)
                     /locus_tag="PLK64_10455"
     CDS             complement(2719..3018)
                     /locus_tag="PLK64_10455"
                     /inference="COORDINATES: protein motif:HMM:NF017793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HPV80212.1"
                     /translation="MAVGDYVKINHGDIEQQATALAAMKNELEENFKRAQAQVVSLNE
                     SGAFQGLSGSSFTEEYAKWTTSITTTLQLMEQFGQHLGKTSKAFAEVDAAFSLKG"
     gene            3453..4043
                     /locus_tag="PLK64_10460"
     CDS             3453..4043
                     /locus_tag="PLK64_10460"
                     /inference="COORDINATES: protein motif:HMM:NF020250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HPV80213.1"
                     /translation="MIGLAVLLIVGSAAIAGLLAMRIDSRVPVLVAAHEIPVGAQITL
                     ADLATQRVASDGLEVLPATAADQLVGKYAAGTIPAGRLLDQAMFAQTGFLRDGAVAVG
                     VSLAPGRMPASGLRAGDRVQVVDVKDGVGVVLVENATVSSAPDTTSSSGAFGGGSTGG
                     SGSVVATLIVPPSSAPGVAASSAANRIAVVLLRRGA"
     gene            4047..4829
                     /locus_tag="PLK64_10465"
     CDS             4047..4829
                     /locus_tag="PLK64_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80214.1"
                     /translation="MALYAVCSAKGSPGVTTLVAAMALGWDRPVVMADLDAGGGDLAL
                     RYRDERGRPLDQDLGLVSLAALSRRSTTTTPIGPHLQVPLGGPPILVGVARPEQVGAL
                     GPAWGPLAQSLAAYEGDVLADCGRVTAGAAVTPVLAAADALLFVVRPTMEELYHLRER
                     LGGLAEQLGSRDMHGRRIAVAVVGSDRNRTGASDVQRLLDASGSGATVVGVFVEDAKG
                     ALRLRYDLAGVPARSVVLRSAGVVGARVREPAGHDLTAAMGV"
     gene            4832..6127
                     /locus_tag="PLK64_10470"
     CDS             4832..6127
                     /locus_tag="PLK64_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPV80215.1"
                     /translation="MTDQLLLRRLREDVADIVSRQRREDAANGVPPMSSEDERQFGRA
                     VISRVLETHARTEMAGGRTPPTMEEEAELADGIHAALFGVGRLQPLLDDPMVENVDIN
                     GCDRVFVGYADGREEMVAPIAESDDELIELVQVLAVYSGLTSRPFDTANPQLDLRLPD
                     GSRLSAVMGVTARPSLSIRRARLSRVFLDDLVVNGSMSSEIAEFLSAAVRARKNIMIA
                     GATNAGKTTLLRALANEIPRNERLITVERAMELGLGQFQDLHPNVVAFEERLPNSEGQ
                     GHIGMAELVRRSLRMNPSRVIVGEVLGDEIVTMLNAMSQGNDGSLSTIHANSSLEVFN
                     RICTYAIQSVERLPADATMMLIAGAIDFVVFVERRNEFHSGGGMRRIITSIREVNGVD
                     GRVLSSEVFAEGSDGVAVPAAAISCIDDLLAAGYSGMSR"
     gene            6153..7040
                     /locus_tag="PLK64_10475"
     CDS             6153..7040
                     /locus_tag="PLK64_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPV80216.1"
                     /translation="MFAQLLVPLVVLFGGLCGVGILLLVKGLTPTEVSLRPRSTLAAD
                     LRRMSIMAGRKVPVAVGAGLFTLVITRWIVLAVSVGLVVFFWGALFGGAAAERAAYER
                     LEGLAAWTESLRDTIAGAVGLEQAIPATANAASPPIARAINDLSDRLRVRVPMTTALM
                     RFADDLDDSTADLVVAALILNAKLRGPGLRDVLSSLASSVRSEVEMRGRISAGRRGTR
                     KSVQIVVAITGLFVIGLRVLNPDYVEPYGTPLGQLVLLLVAGIFALGFYWLRRLATFQ
                     MPARFLQTAPTDLTSGAPR"
BASE COUNT          958 a         2485 c         2468 g         1160 t
ORIGIN      
        1 gcaggccttg gctcccttgc gagacatcac gccgtcgctg gaggatgccg gcctgccgcg
       61 ctcggcccgg ttgttggagt gtgtcgggct gaccgatccg actcccgatg ccgtggtggc
      121 cggatggggg cagccgcaga ccgacgtcgt catcggcgcg ggcttcgacg gaatgttccg
      181 gatcgacctg cgccgcgatg ggccgcacgc gctggtggcc gggacgacgg gctcgggcaa
      241 gtcagagttg cttcagacca tcgtcgcttc gctcgccctg gccaatcgcc ccgatcagtt
      301 gaccttcgtg ctcgtggact acaagggcgg gtcggcgttc aaggagtgca acaagcttcc
      361 gcacaccgtc ggcatggtca ccgacctcga cacgcacctc gtcggccggg cgctgacctc
      421 ccttggtgcc gagttgcgtc ggcgcgagca cctgctcagt gtgccggggg ccaaggacct
      481 cgaggactac tgggcgttgt gtcgcaacga cccgaccttg ccgacgatcc cgcgcctggt
      541 cctggtcatc gacgagttcg ccagcctcaa ggccgagttg cccgacttcg tcaccgggct
      601 cgtcaccatc gcccaacgcg gccgatcact ggggatccac ctcatcctcg ccacccagcg
      661 ccccagcggt gtcgtgtcgg cagacatccg ggccaacacc aacctgcgga tcgccctgcg
      721 ggtcaccgac gagagcgaga gccgcgacgt catcgacgca ccggaggccg ggcggatcct
      781 cgcctcgacc cccgggcgcg gctacgcccg gctcggtccg tcgggcgtcc tgcccttcca
      841 ggccggccga gtcggcggcc ggcgacccga cgccccgagt gagacccgca ccatcccgac
      901 gccgctcgca tgggggattc cgtggtcacg caccggcata ccggcgccgg gtcggcccac
      961 cacggcggcc agcgacaccg acgaagggga gaccgacctg tcggtcctcg tcaccgccgt
     1021 ccgtggggcc gtcgaccacc tgggtatgcc gccccagcac agtccgtggc tgcccgccct
     1081 gcccgggttc gtcaccctcg acgaactccg tgcgcggttg gtggagaggg atccggccgc
     1141 cgcggccgat cccttcaccg tcggttgggc gctggaagac cggcctgagg atcaggccca
     1201 ggtgccggcg accttcaccc tggggacgtc gggacatctt ttcctcgtcg gcgggccccg
     1261 ctcaggacga tccaccgcgt tgcgcacgtt ggtcggcgcg ctggccgaga aggtggcggc
     1321 cagagacctg cacgtctacg gcctggactg cggcaacggt gccctgttgc ccctcaccgt
     1381 gttggcccac acgggggcgg tcgtgcgccg aaccgaggtg gagcgcgcca gccggttgat
     1441 ccggcgattg accgaagagg tcgcccggcg gcagagcgtg ctcggagccc acggctttgc
     1501 cgacatccag gaactgcgca gtgccgcagc gccggacgat cggttgccct atgtgctgct
     1561 cgtgctcgac cggtgggaag gcttcatgtc cgacctcgcc gacgttgatg tcggcagcct
     1621 cctggacggc atggtcacgc tgctgcgcga gggcgcatcg gtcggcatcc aggtcgtcgt
     1681 gacgggcgat cggacgctca actcggcccg ggtcgcctcg ctggtcgagg ccaaactggc
     1741 cctgcggctg cctgatcggc aggactatgc cagcctcggc ctcaaggtgc gagagctgcc
     1801 cgaggagttc cccgagggtc gtggcgtctg gggcgaacgc tccatcgaag cccagatcgc
     1861 cgtcctcggc gcggacgcga gcggccaggg tcagggaacc agcctgcgcg agctcgcggc
     1921 ggcaacggcc gagcgcgacc gcgacctgcc acgcgccctg cgtcccatgg cgttgggcgc
     1981 gctgccggac caagtcgcct gggccgacgt ggccgcggac ttcgaccccg gcgcgcggcc
     2041 gccgtcctgg ctcccgttcg ggatcggcgg cgacctggtc gagcccatcg gcctcgatct
     2101 ggatcggtcg ccggtcgcgc ttgtggcggg ggagtctcgc accggcaaga ccgcgacgct
     2161 gtgcttcctc gcggcatacg cgcggcggtc cgggcgtgcc gtcctcggtt tcgtaccccg
     2221 cgacaacgac ctgtccgcag acctgggtga gggcgcctgc atcgtcggtc aggaggaccc
     2281 caacgcggcg gtggcccggc tccgagacct ccccgagggg gccctcgtcc tcgtcgacga
     2341 cgtcgaggcc tacaaggacg cacccctgac gccgctgatg aacgcgctgg tgcgccaggc
     2401 cgccgaccgg ggcttcacgg tcgtgctctc cgggcgatcc gccgacctgg gggccggcta
     2461 cagcggctgg atgttcgagg ccaggcgggg tcggcatggg ctggtcctgt ccccgtcgga
     2521 ggcgatcgac ggggaattgt tcgggtcacg ggtggcgcgg acggcgttgc ggtcgcgtcc
     2581 gcacgccggg cgcggcgtgc tcttcgactc cacgggagca cagacgacgg tccaggtgcc
     2641 gctggtcgag cggggctgac gcacgaacgc cccggccggt cggccggggc gttcgacgtg
     2701 cggaggtgag gcgttcgctc agcccttgag gctgaaggca gcgtcgacct cggcgaaggc
     2761 cttggaggtc ttccccaggt gctggccgaa ctgctccatc agctgcagcg tcgtggtgat
     2821 cgaggtcgtc cacttggcgt actcctcggt gaacgacgat cccgacagac cctggaaggc
     2881 gccgctctcg ttgagcgaga cgacctgggc ctgcgcgcgc ttgaagttct cttcgagctc
     2941 gttcttcatg gcggcgagcg cagtggcctg ctgctcgatg tcaccgtgat tgatcttgac
     3001 gtagtctcca acagccatga gtgttccctt tcgagttggt atgtcgggaa cagccaaacc
     3061 ccctgagggc gtcggcaacg tcgccgacca gctgaggttc cctttcgtcg aggggatgcc
     3121 tcgacgacga cgacaccatg acacgggtcc ggcgcatggt caatgcgtgc tcctacggat
     3181 gtacaaagtc gtcgggacta ggtagacctg gcttgctgag taggtacacc tgctcgctga
     3241 gtaggtactc ctgcctagtg cgtaggggca tgggctgagc gcctaaactg tgcggtcggt
     3301 agccaaagac cggcggtcat gaccgccacg aatcaggggg cagcaccatc gacgacgtca
     3361 gcacctcacg cacgcgggtc gcggcgggag cggccggggc ggctcgcgcc gtgccacgac
     3421 cggtgtccgc cgctcgccgg cgccgcccag ccatgatcgg gttggcggtc ctgctcatcg
     3481 tcggttccgc agcgatcgcg ggcctgctcg cgatgcggat cgacagccgg gtcccggtgc
     3541 tcgtcgccgc tcatgagatc cctgtcgggg cgcagatcac cctcgccgac ctggcgaccc
     3601 agcgggtcgc cagcgatggc ctggaagtcc tccccgctac tgccgcagac caactcgtcg
     3661 gcaagtacgc cgcgggcacc atcccggcgg gacggctgct cgaccaggcg atgttcgccc
     3721 agaccggttt cctgcgggac ggcgcggtcg ccgtgggtgt ctcgctcgcg cccggtcgga
     3781 tgccggccag cggcctgcgc gccggagacc gggtccaggt cgtcgacgtc aaggacgggg
     3841 tcggcgtggt cctcgtcgag aacgccacag tgtcctcggc ccctgacacc acgtcctcct
     3901 caggggcgtt cgggggaggc tcgaccggcg ggtccggcag cgtcgtcgcg acgctgatcg
     3961 tcccgccgtc ctcggctccc ggggtcgccg cctcgtctgc ggccaaccgc atcgccgtgg
     4021 tcctgctgcg ccgcggggcc tgacccatgg ccctgtatgc cgtgtgctcg gccaaggggt
     4081 caccgggcgt gaccaccctc gtcgccgcga tggcgctggg ctgggaccgt cccgtcgtca
     4141 tggctgacct cgatgccggc gggggagacc tggccctgcg ctaccgcgac gagcgagggc
     4201 gtcccctcga tcaggatctc gggcttgtct cgctggccgc gttgtcgagg cgctcgacca
     4261 cgacgacgcc gatcggcccg cacctgcagg tgcctctcgg aggcccgccg atccttgtcg
     4321 gcgtcgcccg ccctgagcag gtcggtgccc tgggcccggc gtggggcccg ctggcccagt
     4381 cgttggcggc atacgaaggc gacgtcctgg ccgactgcgg tcgggtcacg gccggcgcgg
     4441 ccgtcacccc ggtcctcgcc gccgcggacg cgctgctctt cgtcgtgcgt ccgacgatgg
     4501 aggagctcta ccacctgcgt gagcgcctcg gcggtctggc cgaacagttg ggctcgcggg
     4561 acatgcacgg tcgacggatc gccgtcgctg tcgtcggctc cgaccgcaac cgcaccggcg
     4621 cttccgacgt ccagcggctg ctcgacgcgt cggggtccgg agcgactgtt gtcggggtgt
     4681 tcgtcgagga cgccaaggga gccctccgcc tgcgctacga cctggccggg gttcctgccc
     4741 ggtcggtggt cctgcgctcg gcgggcgtcg tgggcgctcg cgtgcgcgag ccggccggcc
     4801 acgatctgac cgcagcgatg ggggtctgat cgtgaccgat caactcctgc tccggcggct
     4861 gcgcgaggac gtcgccgaca tcgtttccag gcaacggcgt gaagacgccg ccaacggcgt
     4921 gccgccgatg tccagcgagg acgagcgtca gttcggccga gccgtcatct cgcgggtgct
     4981 cgagacccac gcccgcaccg agatggccgg cggtcgcacc cctccgacga tggaggagga
     5041 ggccgagctc gccgacggca tccatgcggc cctcttcggc gtcgggcggc tgcagccgct
     5101 gctggatgac cccatggtcg agaacgtcga catcaacggc tgcgaccggg tcttcgtcgg
     5161 ctatgccgac gggcgcgagg agatggtcgc gccgatcgcc gagtccgacg acgagctcat
     5221 cgagctggtc caggtccttg cggtctactc cggcctgacc agccggccct ttgatacggc
     5281 caacccgcaa ctggacctgc gcctgccgga cggaagccgg ctctcggccg tcatgggcgt
     5341 cacggcccga ccgtcgctgt cgatccgtcg cgcccgcctc tcgcgggtct tcctcgacga
     5401 tctcgtcgtc aacgggtcga tgagcagcga gattgccgag ttcctctcgg cggccgtccg
     5461 ggcccgcaag aacatcatga tcgcgggggc caccaacgcc ggaaagacca cgctgctgcg
     5521 ggccttggcc aacgagatcc cccgcaacga acgcctcatc accgttgaac gcgcgatgga
     5581 gctcgggctg ggtcagttcc aggatctgca ccccaacgtc gtcgccttcg aggagcggtt
     5641 gcccaactcc gagggccagg gtcacatcgg catggccgag ttggtgcgtc gttcgctgcg
     5701 tatgaacccc agccgggtca tcgtcgggga ggtcctcggc gacgagatcg tcacgatgct
     5761 caacgccatg agccagggca acgacgggtc gctatcgacg atccacgcca acagctcgct
     5821 cgaggtgttc aaccggatct gcacctatgc gatccaatcc gtggagcgcc tgccggccga
     5881 cgcgaccatg atgctcatcg ccggcgcgat cgacttcgtc gtcttcgtcg agcgacgtaa
     5941 cgagttccac tccggcggtg ggatgcgacg aatcatcacc tcgatccgcg aggtcaacgg
     6001 tgtcgatggc cgggtgctct cgagcgaggt cttcgccgag ggatccgacg gtgtcgccgt
     6061 cccggccgcg gcgatcagtt gcatcgatga cctgctcgcc gcgggttact cgggcatgag
     6121 ccgatgacgg cgggccgacg ccgatgaacg gcgtgttcgc acaactcctg gtcccgctcg
     6181 tcgtgctgtt cggcgggctc tgcggcgtgg ggatcctgct gctcgtcaag gggttgacgc
     6241 ccaccgaggt ctcgctgcga ccgcgcagca ccctcgcggc cgacctgcgt cggatgtcga
     6301 tcatggccgg ccgcaaggtc ccggtcgcgg tcggggccgg gctgttcacc cttgtgatca
     6361 cccgctggat cgtgctggcc gtgagcgtcg ggctggtcgt cttcttctgg ggcgccctgt
     6421 tcgggggagc ggcagccgag cgcgcggcat acgagcgcct ggagggcctc gcggcgtgga
     6481 ccgagtcgtt gcgcgacacc attgccggcg cggtcgggct ggagcaagcg atcccggcca
     6541 ccgccaatgc ggcgtcgccg cccatcgctc gggcgatcaa cgacctgtcc gatcgactgc
     6601 gggttcgtgt gccgatgacg accgccctca tgcgcttcgc cgacgacctg gacgactcga
     6661 cggcagacct cgtcgtcgcg gcgttgatcc tcaacgccaa actgcgcggg ccaggtctgc
     6721 gggacgtgct ctcgtcgctg gcgtccagtg tgcgcagcga ggtggagatg cgcggccgga
     6781 tcagcgccgg tcggcgcggc acccgcaaga gcgtgcagat cgtcgtcgcc atcaccgggt
     6841 tgttcgtcat cggtctgcgg gtgctcaacc ccgactatgt cgagccttat ggcacccccc
     6901 tcggccagct cgtgctgctg ctcgtcgcag ggatcttcgc gctcgggttc tactggctgc
     6961 gccggttggc caccttccag atgcccgctc gcttcctgca gactgcgccc accgacctga
     7021 cgtcgggagc cccccgatga ccctgacact cgtgctcatc gccggagcca t
//
LOCUS       DAOMZY010000177         7065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_10001_length_7065_cov_6.514123, whole genome shotgun sequence.
ACCESSION   DAOMZY010000177 DAOMZY010000000
VERSION     DAOMZY010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7065
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10001_length_7065_cov_6.514123"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PLK64_10480"
     CDS             complement(<1..331)
                     /locus_tag="PLK64_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017200910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HPV80217.1"
                     /translation="MDTLFAGYAARERTFDEMFEAAPNEVRSGYTDVHNRLTRMSPAD
                     VRGRADFLARIYVEQGVTFDIGGEERPFPLDIVPRLLDADTWATVEVGLTQRVRALER
                     FLDDVYGP"
     gene            564..1346
                     /locus_tag="PLK64_10485"
     CDS             564..1346
                     /locus_tag="PLK64_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPV80218.1"
                     /translation="MNIVVCVKYVPDAQAERTFNASDSTTDRVNVDGLLSELDEYAVE
                     EALKIVEAGEGEVTVLTIGPDEASDAIKKALQMGADKGVHVIDDAIHGSDALATSLLL
                     AKAIEKIGVPDLIVTGMSSTDGIMGVVPTMLAERLGLPAVTFASELTVGSGTVSARRD
                     GDVASQTIEAALPALVSVTDQINEPRYPSFKGIMAAKKKAVETWTLADLGVSADEVGL
                     DAAWTKVTSFEARPPRAKGTIVKDEGDGGAKLAGFLAERKFI"
     gene            1377..2333
                     /locus_tag="PLK64_10490"
     CDS             1377..2333
                     /locus_tag="PLK64_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPV80219.1"
                     /translation="MAEVLVLVDHVDGALRKTTTELLTIARRLGEPSAVFIGSGFDAA
                     KEGLAAYGAEKVYRIDADLSGFLVVPQAEALAAVAAQASPAAVLVASSPEGKEIAARL
                     AVKLGSGLITDAVDVQSDGAGGVSTTQTVFAGSYTVKATVTKGTPVIAVKPNSAAPEA
                     VAGAAAEVTVPFEPSALAQSAKIVSAKPKEKTGRPELTEAAIVVSGGRGTGGDFSKVE
                     AFADSLGAAVGASRAAVDAGWYPHSHQVGQTGKQVSPQLYVACGISGAIQHRAGMQTS
                     KTIVAVNKDEEAPIFELVDFGVVGDLFAVLPQATDAVAAAKA"
     gene            2437..2979
                     /locus_tag="PLK64_10495"
     CDS             2437..2979
                     /locus_tag="PLK64_10495"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPV80220.1"
                     /translation="MDLLLLRHQPTLWGLAVRLDPMGPEHYDGLRPLFVGDSDSPLAF
                     VLAEKVRVGLAVAKDRPDRADWAIVRIADQRVVGEVVLFSLDADHDAMEFRIALVGPE
                     EFGHGYGTEATRLVRDFAFGPLGLRRVSLEVGASNARARRVYDRAGFIVEGVRRQVRL
                     VDGVWDDVVDMAMLASDPRP"
     gene            complement(3012..3824)
                     /locus_tag="PLK64_10500"
     CDS             complement(3012..3824)
                     /locus_tag="PLK64_10500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HPV80221.1"
                     /translation="MTHTGPSGTRIAAFFDLDGTLIPGSANIPLAKAAFGAGLVSKRE
                     LALDLARNASFMLRGASDDSSAKVRDRILRGVKGHPGSEVERLADGFLDDLVATITPT
                     MREVLAQHRAQGHELVLVSASPTEIVLRFAVAAGMDYGVGTTAGRDEAGCFDGTLAGP
                     FCYKEGKAEVIRGLAATHGYDLAQCFAYSDSVSDEPMLAIVGNPVAVNPDHELRLMAL
                     AGGWRIVEVGRLRVAVNRIRRAARSAITSPGKAWTRGRAELTRRREPIADSE"
     gene            3981..5165
                     /locus_tag="PLK64_10505"
     CDS             3981..5165
                     /locus_tag="PLK64_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005270317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPV80222.1"
                     /translation="MPVYLDHAATTPVFPDVAAAMSAQRDRVGNASSLHTAGRAARRV
                     VEESRERLAGVLGARPSEVVFTSGGTESDNLAVAGVYRARRDADPRRRRLLVSAIEHH
                     AVLDCVQALVDREGAEVDWLPCDADGVVQVADVRAAIAADPQSVALVSVMWANNEVGT
                     VQDVAAIAAEAHAHGIPMHTDAVQAVGHLPVDFLASGVDLLTLTGHKVGGPIGSGALL
                     VRREQDVVPLVYGGGQERQIRSGTLDTVGILGLSLAVEASVGMLPVEGPRLCRLRDDL
                     IEGAMGLGYDIRPGGMWRPGDWASRSAGNAHLLVPGCEGDSLLYLLDAAGVECSTGSA
                     CQAGVPQPSHVLLAMGLSEREARGALRLTLGHTSTGADVEAVLAALPAALDRARRAAG
                     VA"
     gene            5165..6262
                     /gene="mnmA"
                     /locus_tag="PLK64_10510"
     CDS             5165..6262
                     /gene="mnmA"
                     /locus_tag="PLK64_10510"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPV80223.1"
                     /translation="MRVVAAMSGGVDSAVAAARLIDAGHEVVGVHLALSEDARAVREK
                     ARGCCTLEDSADARRVADRLGIPFYVWDLSARFRTEVVEDFVAEYAAGRTPNPCLRCN
                     ERIKFAALLDKAVALGFDAVGTGHYAQVVEGPNGRELHRAVDAAKDQSYVLGVLDAGQ
                     LAGAFFPLGGSTKAQVREEAAARGFAVARKPDSHDICFIPDGDTRGWLAGRLGSRPGV
                     VVDESGAVVGSHGGAYGYTVGQRKGLALPRPAADGLPRYVQSVDVATNTVVVAPGELL
                     GVDVIEGEHLRWCGRPPTGATRVEAQVRAHGEGLPGVATVLGAAVGQTVQVHLDASVR
                     GVAPGQSVVLYDGTRVVGSATITATGRRSRA"
     gene            6402..>7065
                     /locus_tag="PLK64_10515"
     CDS             6402..>7065
                     /locus_tag="PLK64_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HPV80224.1"
                     /translation="MTSASGIGSWPGTDVAEALRIQRELLGDHDEQADGVRGIPSLPE
                     LPARGPGGDIIGRTAGLLVDLAVDLQPAGWRFVDRPGRDAARTAAFWGEDLDELAAAF
                     DGYAGPLKVAVAGPFTLAADIWLHRGERAIVDPGATRDLSESLAEGLRTLLVRLTRLV
                     PGAELIVQVDEPSLPAVLDGRLPTASGFGRLRAIDVQDALPALQTVLAAAGEHGTIVH
                     CCA"
BASE COUNT         1007 a         2334 c         2596 g         1128 t
ORIGIN      
        1 ccggcccgta gacgtcgtcg aggaaccgct cgagggcccg cacccgttga gtcagcccca
       61 cctcgaccgt cgcccaggtg tcggcgtcga gcaaccgcgg cacgatgtcc agcgggaacg
      121 gccgttcctc gccgccgatg tcgaaggtca cgccctgctc gacatagatg cgagccagga
      181 agtccgcacg cccacgcacg tccgcaggac tcatccgggt caaccggttg tggacgtcgg
      241 tgtaccccga tcgaacctcg ttcggcgccg cctcgaacat ctcgtcgaag gtgcgctcgc
      301 gggcggcata cccggcgaat agcgtgtcca cgagggagga cgttaaggcc gaacgatttc
      361 caactcgttt cgtccgcgtt ccgtcgtgga gacatcgccc gcgcaggtat gccgtgccga
      421 cggtcacgtg ggtcgcgcgt gagtcgcgtc acgcggcata ccgtgaggtg gtgggcatca
      481 cgcccagggc ggctgggagt gtcggcctga cctgacagga tggtccagac cctaaccagc
      541 acacgggaag aggacgtgtc tagatgaaca tcgtcgtctg cgtgaagtat gtgcccgacg
      601 cgcaagcaga gcgcaccttc aacgcctccg acagcacgac cgatcgcgtc aacgtcgacg
      661 ggttgctctc cgagctcgac gagtatgccg tcgaggaggc gctcaagatc gtcgaagcgg
      721 gcgaaggtga ggtcactgtc ctcaccatcg gacccgacga ggcatccgac gcgatcaaga
      781 aggccctgca gatgggcgcc gacaagggag tgcacgtcat cgacgacgcc atccacggct
      841 ccgacgctct cgcgacctcg ctgttgctgg ccaaggcgat cgagaagatc ggcgtgcccg
      901 acctcatcgt cacggggatg tcctcgaccg acggcatcat gggcgtcgtt ccgacgatgc
      961 tcgccgagcg cctcggcctg ccggccgtga ccttcgcgtc cgagctcacc gtgggttccg
     1021 gcaccgtgtc ggcgcgccgc gacggtgacg tcgccagcca gaccatcgag gccgcgctgc
     1081 cggccctcgt gtcggtgacc gaccagatca acgagccgcg ttacccctcc ttcaagggga
     1141 tcatggcggc gaagaagaag gccgtcgaga cgtggacgct ggccgacctg ggcgtgtccg
     1201 ccgacgaggt cggcctcgat gccgcctgga ccaaggtgac ctctttcgag gcgcggccgc
     1261 cgcgcgcgaa gggcaccatc gtcaaggacg agggcgacgg cggagccaag ctcgctggct
     1321 tcctcgccga gcgcaagttc atctgagcgg cgacgcggga ataggagatc actgacatgg
     1381 ctgaagttct cgtcctcgtc gaccacgtcg acggcgcgct gcgcaagacg accaccgaac
     1441 tgcttaccat cgcccggcgg ctcggcgagc cgtcggccgt cttcatcgga tccggcttcg
     1501 atgccgccaa ggaggggctg gcggcatacg gtgcggagaa ggtctaccgc atcgacgccg
     1561 acctgtccgg cttccttgtg gtgccccagg ccgaggccct cgccgcggtc gccgcccagg
     1621 cgtccccggc agcggtgctc gtcgcgagtt cacccgaagg caaggagatc gccgcgcgct
     1681 tggcggtcaa gctgggctcc gggctcatca ccgacgcggt cgacgtgcag tccgacggag
     1741 ccggtggggt ctcgacgact cagaccgttt tcgccggttc ttacaccgtc aaggcgaccg
     1801 tcaccaaggg cacgccggtt atcgcggtca agcccaattc ggctgcgccc gaagcggttg
     1861 ctggtgctgc tgccgaggtg accgtcccct tcgagccgtc ggcgctggcc cagtcggcca
     1921 agatcgtgtc ggccaagccg aaggagaaga cgggtcgccc cgagctcacc gaagctgcca
     1981 tcgtcgtctc gggcggacgt ggcaccggtg gcgacttctc caaggtcgaa gccttcgccg
     2041 actccctcgg cgcagcggtc ggtgcgagcc gcgccgctgt cgacgccggc tggtacccgc
     2101 acagccacca ggtcggccag accggcaagc aggtcagccc ccagttgtac gttgcgtgcg
     2161 gcatctccgg tgcgatccag caccgggccg gcatgcagac ctcgaagacg atcgtcgccg
     2221 tcaacaagga cgaagaggcg ccgatcttcg agctggtcga cttcggagtg gtcggcgacc
     2281 tgttcgccgt gctcccgcag gcgaccgacg cggtggcggc ggccaaggcc tagtcgcgca
     2341 cgcggcatac gctgcggggt ccggaccggt ggtggtccgg gccccgcttc cgtatgccgg
     2401 cttgccgccg cgcgctggga ttggttgact ccgcccgtgg atctcttgct gttgcggcac
     2461 cagccgaccc tgtggggcct ggccgtacgc ctcgatccga tggggcccga acactacgac
     2521 gggctgcggc ccttgttcgt gggcgactcc gacagcccgt tggctttcgt gctggccgag
     2581 aaggtccgcg tcgggctggc cgtcgccaag gaccgacccg atcgggccga ttgggccatc
     2641 gtgcggatcg ccgatcagcg ggtcgtgggg gaggtcgtgc tgttctccct cgacgccgac
     2701 cacgacgcga tggagttccg catcgcgctc gtcggcccgg aggagttcgg ccatggctac
     2761 gggacggagg cgacccgtct ggtgcgggac ttcgccttcg ggccgctggg gttgcgccgg
     2821 gtgagcctgg aagtcggcgc gagtaatgcg cgggcgcggc gggtgtatga ccgggcgggc
     2881 ttcattgtcg agggagtgcg ccgtcaggtg cggctggtcg acggggtgtg ggacgacgtg
     2941 gtcgacatgg cgatgctggc ctcggacccg aggccctgaa agacccgagg ccctgaaaga
     3001 cccgagggct gctactcgga gtcggcgatg ggctcgcgtc gccgggtcaa ctcagctcgc
     3061 ccgcgggtcc acgccttgcc cggcgaggtg atcgccgaac gagccgcccg gcggatccgg
     3121 ttgacagcaa cccgcaggcg gccgacctcg acgatccgcc aaccgccagc caatgccatc
     3181 agccgcagtt cgtggtcggg gttgacggcg accgggttgc cgacgattgc cagcatcggc
     3241 tcgtcactca ccgagtccga atacgcgaag cactgcgcca agtcgtagcc atgggtcgcg
     3301 gcgagcccgc ggatgacctc cgccttgccc tccttgtaac agaacggccc ggccagggtg
     3361 ccgtcgaaac accccgcctc gtcccgaccg gcagtggtcc ccacgccgta gtccatgccc
     3421 gccgccaccg cgaaacgcag aacgatctcg gtcgggctcg ccgacacgag gacgagctcg
     3481 tgaccctgag cgcgatgttg ggcgagcacc tctcgcatcg tcggggtgat cgtggcgacc
     3541 aggtcgtcga gaaagccatc ggcgagccgt tcaacctcgg agccagggtg tcccttgacc
     3601 ccgcgcagga tccggtcgcg gaccttggcc gagctgtcgt cactggcgcc gcgcaacatg
     3661 aacgacgcgt tgcgcgccag gtcgagggcc aactcccgct tgctcaccag ccccgcgccg
     3721 aaggcagcct tggcgagcgg aatgttggcc gacccgggaa tgagcgtgcc gtccaggtcg
     3781 aagaacgccg cgatacgcgt gccagagggg ccggtgtggg tcatgctcgc tgagctcctc
     3841 gggacgggga cgaaccagca catcatgacg caggtcgcgg ttgcttgcca gggttctccc
     3901 tcccctttcc gccccgtgac cgaccgtcca cggtatgccg cgtcgcggcg gcatactcac
     3961 gcgcggctta ggatcggcgg gtgcccgtgt acctcgacca tgccgcgacg acgccggtct
     4021 tcccggacgt cgcggctgcg atgtcggcgc aacgggatcg ggtcggcaac gcctcgtcgt
     4081 tgcacactgc gggccgggct gcccggcggg tcgtcgagga gtcccgcgag cggttggccg
     4141 gagtgctcgg cgctcggccg tccgaggtgg tgttcacctc cggggggacc gagtcggaca
     4201 acctcgccgt ggcgggcgtc tatcgggctc gtcgagacgc agacccccgg cggcgtcggc
     4261 tgcttgtcag cgcgatcgag caccacgccg tgctcgactg cgtgcaggcg ctggtcgacc
     4321 gcgagggcgc cgaggtcgac tggctgcctt gcgatgccga tggtgtcgtt caggtcgccg
     4381 acgtgcgcgc cgccatcgcg gcagacccgc agtcggtcgc actcgtctcg gtgatgtggg
     4441 ccaacaatga ggtcggcacc gtgcaggacg tggcggcgat cgccgcggag gcgcacgcgc
     4501 acggcatacc gatgcacacg gacgcggtcc aagcggtcgg tcacctaccc gtggacttct
     4561 tggccagcgg cgtcgacctg ctcaccctga ccgggcacaa ggtgggcggg ccgatcgggt
     4621 ccggcgcctt gctcgtgcgg cgggagcagg acgtcgtgcc gttggtctac ggcggcggcc
     4681 aggagcgcca gatccgttcc gggaccctcg acactgtcgg gatcctcggg ttgtccctcg
     4741 cggtcgaggc gtccgtaggg atgcttccgg tggagggacc gaggctgtgt cggttgcggg
     4801 acgacctcat cgagggcgcg atggggctgg gttatgacat ccggcccggt gggatgtggc
     4861 ggccgggcga ctgggcgagc cggtcggccg gcaacgcgca cctgctcgtc cccggatgcg
     4921 agggtgactc gttgctctac ctgctcgacg cggccggggt cgagtgctcg acagggtcgg
     4981 cctgccaggc cggggtgccg cagcccagcc acgtcttgtt ggccatgggg ttgagcgagc
     5041 gcgaggcgcg gggagccctg cgcttgacct tggggcatac ctccacgggg gcggacgtcg
     5101 aggcggtcct ggccgcattg cctgcagcgc tcgaccgagc acgtcgggct gctggggtcg
     5161 cctgatgcgc gtcgtcgcgg cgatgagcgg cggggtcgac tcggcggtcg ccgccgcgcg
     5221 gttgatcgac gccgggcacg aggttgtcgg ggtgcacctg gcgctgtccg aggacgcccg
     5281 ggcggtgcgc gagaaggccc gaggctgctg cacgctggag gactcggccg acgcgcgccg
     5341 cgtcgccgat cggctgggca tccccttcta cgtctgggac ctgtcggcgc gcttccgcac
     5401 cgaggtggtc gaggacttcg tcgccgagta tgccgcgggc cgcaccccca acccctgcct
     5461 gcgctgcaac gagcggatca agttcgccgc gctcttggac aaggcggtgg cgctgggctt
     5521 cgatgcggtc gggaccggtc actacgcgca ggtcgtggag ggtccgaatg gtcgtgagtt
     5581 gcatcgggcc gtcgatgcgg cgaaggacca gtcctatgtg ctcggggtgc tcgatgccgg
     5641 ccagttggcc ggggcgttct tcccgctggg tgggtcgacc aaggcgcagg tgcgggaaga
     5701 ggctgcggcg cgtgggtttg cggtggctcg gaagcccgac tcgcacgaca tctgcttcat
     5761 ccccgacggt gacacgcggg ggtggctggc ggggcggttg ggctcgcgcc ctggcgtcgt
     5821 ggtcgacgag tcgggtgcgg tggtggggtc gcatggcggc gcctacgggt acacggtggg
     5881 ccagcgcaag ggactggcgc tgcctcggcc ggccgccgac gggctgccgc gttatgtgca
     5941 gtccgtcgac gtcgcgacca acacggtggt cgtcgcgccg ggggagttgc ttggggtgga
     6001 tgtcatcgag ggcgaacacc tgcgctggtg tggccggccg ccaacggggg cgactcgggt
     6061 ggaggctcag gtgagggccc acggtgaggg cttgcccggg gtggcgaccg tcttaggcgc
     6121 cgcggtgggg cagaccgtgc aggtgcatct cgacgcctcg gtgcgtgggg tcgctccggg
     6181 acagtcagtg gtgctttatg acgggactcg ggttgtcggt tcggcgacga tcacggcgac
     6241 gggtcggcgc tcccgggcgt aggcctaggg ctgcgaaacc atcggcgggt gtggggaatt
     6301 cggggccaac cggcatacct ctcgcccaga acaccggcga gtgtgaggat ttcggaggag
     6361 cccgggcgta ggccaagggg gcggcagtag gctcaagggc tgtgacctct gcgagtggga
     6421 tcggctcttg gccggggacc gatgtcgccg aggccctgcg gatccagcgg gagttgctcg
     6481 gcgatcacga cgagcaggcg gacggggtgc gcggcatacc gtcgctgcct gagttgcccg
     6541 ctcgcggacc gggcggggac atcatcggcc gcaccgccgg gttgctcgtg gacctcgccg
     6601 tcgatttgca accggcggga tggcgtttcg tcgatcgacc gggccgagac gctgcgcgga
     6661 cggccgcctt ctggggcgag gacctcgacg agttggccgc cgccttcgac gggtatgccg
     6721 gtccgctcaa ggtcgcggtg gccggaccgt tcaccctcgc ggcggacatt tggctgcatc
     6781 gcggcgaacg cgccatcgtc gatccgggtg ccacccgcga cctgtccgag tcactcgccg
     6841 agggactgcg caccctcctg gtccgactga cccggctcgt gcctggggct gagctcatcg
     6901 tccaggtcga cgagccctcc ctgcctgcgg tgcttgacgg gcgattgccc acggcatcgg
     6961 ggttcggcag gttgcgggcc atcgacgtcc aggacgcgct acctgccctg cagacggttc
     7021 tggcggcggc gggcgagcac gggacgatcg tgcattgctg cgcgg
//
LOCUS       DAOMZY010000178         7027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_10069_length_7027_cov_6.233219, whole genome shotgun sequence.
ACCESSION   DAOMZY010000178 DAOMZY010000000
VERSION     DAOMZY010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10069_length_7027_cov_6.233219"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PLK64_10520"
     CDS             complement(<1..440)
                     /locus_tag="PLK64_10520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006214294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV80225.1"
                     /translation="MTEVRVREAVPADCPAIYELVVALAVYEKEPDAVEGSVDDMRAH
                     LFGPTPQVFCHVAEVSDDAGGWSVAGIAIWYVTFSTWKVRHGIWLEDLFVRPEARGLG
                     LGRLLLTELAGIAVERGYPRFEWWVLDWNTPAQGFYESLGAAPQ"
     gene            complement(1206..2573)
                     /locus_tag="PLK64_10525"
     CDS             complement(1206..2573)
                     /locus_tag="PLK64_10525"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HPV80226.1"
                     /translation="MSTRVWWLRRDLRVADHPALLTAADGGAVVPLFVLDPALWAAAG
                     APRQARLLASLRSLDADLRDRYAVSLVVRVGSPLTVVPALATEVGAVAVHVTAETTPY
                     GRRRDTAVRAALNAQGRELVATGTAYAIGPGTVRTGGGTPYRVFTPFARAWRALGAPG
                     PAPAPSRLIGLRAVPSDDLPLVESSGVAAEGVVAGESAARVRWEEFLRDGVADYGERR
                     DRPDLDGTSRLSAALKFGEVHPRTLLADLARSPAAGSPGATTFVTELAWREFYADVLW
                     HHPASAWSDLRPMAGMVYDEPSAGFEAWRAGRTGFPFVDAGMRQLAATGWMHNRVRMV
                     TASFLVKDLHVWWPHGARHFMALLADGDLASNSHGWQWCAGTGTDAAPYFRVFNPVGQ
                     GLRFDPDGAYVRRWVPELAHLPGAAAHEPWRHPRGYAASYPTRILDHAVEREEALRRY
                     AATRT"
     gene            2593..3339
                     /locus_tag="PLK64_10530"
     CDS             2593..3339
                     /locus_tag="PLK64_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HPV80227.1"
                     /translation="MTDMIDTTEMYLRTIFELEEDGIPPLRARIAERLGHSGPTVSQT
                     VARMERDGLLAVTDHRHLELSESGRRAATRVMRKHRLAERLLVDVLGLEWELAHAEAC
                     RWEHVISDEVERRILALVGDSTRSPYGNAIPGLAELGGQVGRDTTAERDSEEPLTEVL
                     GVSDHGPIAGAKASGESVVVLTSRIGEPVQVDHTALSLLTVAGLVPGRSARVSLQGNE
                     IRAIPEGVADREPVVVPTEVAAYVFVFRRA"
     regulatory      3527..3703
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            3706..4560
                     /locus_tag="PLK64_10535"
     CDS             3706..4560
                     /locus_tag="PLK64_10535"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HPV80228.1"
                     /translation="MAKHVGRHRAPGFSPLTELTQIAGDSAKPAVRATAVMAVSTGML
                     AGMVVPAQAATIVAGVDGVSSDGQALDTPVSDLLGISGLPSTDGPGLAPAAPAPVAAT
                     GLVEAVEAAVGPVVTAPDTVAGLPTFGALGFKTKTITVPAVTQVSRSTAPRTSPAPAA
                     KAATPAPAPAPVSGGSVLSVAASLLGIPYRYGGSSPSTGFDCSGFTSYVYRIARGITI
                     PRTAEGQRKGSARVTSPVPGDLVFFGVPATHVGIYAGNGMMYDSPHTGTVSQLRKIYS
                     GNVSYGRF"
     gene            complement(4636..4905)
                     /locus_tag="PLK64_10540"
     CDS             complement(4636..4905)
                     /locus_tag="PLK64_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80229.1"
                     /translation="MSTSTTTTPTTTTERSGFWASVRDDLRERREARAIRAQMFRELE
                     GYQSRGDILDLLAAADRHEGPEAELMRGVLQSKLADVNRSSRRAS"
     gene            4984..5928
                     /locus_tag="PLK64_10545"
     CDS             4984..5928
                     /locus_tag="PLK64_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPV80230.1"
                     /translation="MDTEVLRWFQQVADGATVTEVADLWMVSQPKVSRDLMALQREVG
                     APLLVKSGRVLRLTHAGAVFKRHADAIVNRLDDALAAVDQLVDPERGTVTLAYERTLG
                     TWLVPSLVGEFLAVHPQVSFTLVVGGEDLAVLDEGRVDLAVLASRPNAPLFGGAREGG
                     GASEASVRWEPLFREPLFLAVGPGHRFEDIETVPLAELADEPWVVLPPGTVIRSALDQ
                     LCHGAGFEPKVAFECDDLATVRGFVGAGLGVSIVPAMGEDPLPRYAAAPRLVALADAG
                     ARREVGLAWSSQRRLLPAAELFRTFVLRSSGAGRTIAS"
     gene            5957..6292
                     /locus_tag="PLK64_10550"
     CDS             5957..6292
                     /locus_tag="PLK64_10550"
                     /inference="COORDINATES: protein motif:HMM:NF026933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm51 family immunity protein"
                     /protein_id="HPV80231.1"
                     /translation="MDPLNLIETAPDDVALTMYTGELVVDAMVRKLGHEPNGTFWDGV
                     AEWLVETEAPTLEGRFDYDSEGGMFCAYGDRADLEALRALMLPVVTDPKRVRSLIKAA
                     EDADFEFDD"
BASE COUNT         1014 a         2432 c         2480 g         1101 t
ORIGIN      
        1 tcctgcggtg cggcgcccag gctctcgtag aacccctgcg ccggcgtgtt ccagtccaac
       61 acccaccact cgaacctcgg gtagccccgc tccaccgcga tcccggccaa ctcggtgagc
      121 agcagccgcc ccaggcccag cccccgcgcc tcgggccgca cgaacaggtc ctccagccag
      181 atcccgtgcc ggaccttcca ggtcgagaac gtgacgtacc agatggcgat ccccgcgacg
      241 ctccacccgc ccgcgtcgtc actcacctcg gcgacatggc aaaagacctg cggcgtcggc
      301 ccgaacaggt gcgcgcgcat gtcatcgacg ctgccctcga ccgcgtcggg ctccttctca
      361 tagaccgcca gcgccacgac gagctcgtag atcgccgggc agtcggcagg gacggcttca
      421 cgcacacgca cctcagtcac gaccccgagg gtatgccgtc cgcccaccga cgtagggccg
      481 gccgttggcg catgttgctg ggtggggcga ccgccgcctc cacgccagtg tctggagcaa
      541 agccgatgag caacgtcatc tacacgtatg ccacgcccga gggcgcaccg aagggcaccc
      601 gggagcgaag ggtcctcgac tcgaggccca aggtcgacat cgacctcgaa ccgtccggcc
      661 atggggttgc ctctggcgat cggcgcgaga gtgatccgag ctgcgaggcg gtggatagct
      721 gcacttcgcc gctcttgggc gcctgtgctg gactccatca gatccgccgc ctcatgtgcg
      781 aggtcgatcg cctccgcgcc gaggttgctg gcctccgcgc ggaaatcaag agatgcggcc
      841 gacacccgga catcgggtcc tgatcgagcc ggcactcgtt gctgggctgg tcggaatgac
      901 tcgctcgacc gcccacccca ccgccgccgg cgcacatccc gagcctcggt gagccgagtg
      961 cgggacggag tgcgtttggt ggggtgatca cccccgtaat cgcactctgt cgcgccctca
     1021 agtgcggggc tggggtaacc accctgccca ggccacgaat caggcgccac cgccagtcga
     1081 gtgcgttacg gagtgcggtt ggtggggtga tcaccccctt gatcgcactc ggtcccgcgc
     1141 tcgctggggg ccagtgttag ttcggcggta tgagtgcgac cgaaaccgat gcaggagtcg
     1201 agcgctcagg tgcgggtcgc ggcataccgg cgcagggctt cctcgcgctc gacggcgtgg
     1261 tcgaggatcc gggtggggta gctcgcggca taccccctcg ggtggcgcca cggctcgtgc
     1321 gcagccgcgc cgggcaggtg ggcgagctcg gggacccaac ggcgcacata agcaccgtcg
     1381 gggtcgaacc gcagcccctg cccgaccggg ttgaagaccc ggaagtaggg ggcagcgtcg
     1441 gtgccggtgc cggcgcacca ctgccagccg tgcgagttgg aggcgaggtc accgtcggcc
     1501 agcagcgcca tgaagtgccg ggcgccgtgc ggccaccaca cgtgcaggtc tttgacgagg
     1561 aagctcgccg tcaccatccg cacccggttg tgcatccagc cggtcgcggc caactggcgc
     1621 atccccgcat cgacgaacgg gaacccggtg cgccccgccc gccacgcctc gaagcccgca
     1681 ctcggctcgt cataaaccat ccccgccatc ggccgcaggt cgctccacgc cgaggccggg
     1741 tgatgccaca acacgtcggc gtagaactcc cgccaggcca actcggtgac aaaggtcgtc
     1801 gcgcccgggc tgccggccgc aggggagcgg gcgaggtcgg ccagcaacgt gcgcggatgc
     1861 acctcgccga acttcagcgc cgcggacagc cgcgaggtgc cgtcgaggtc cggccggtcg
     1921 cggcgctcgc cgtagtcggc cacgccatcg cgcaggaact cctcccagcg tacgcgggcg
     1981 gccgattcgc cagccaccac accctccgcc gccacaccag aactctcaac cagcgggagg
     2041 tcgtcggagg ggacggcgcg cagcccgatc aggcgactcg gtgcgggcgc cggcccaggc
     2101 gcccccagcg ctcgccaagc gcgcgcgaac ggcgtgaaca cccgataggg cgtcccacct
     2161 cccgtccgca ctgtcccagg cccgatcgcg tatgccgtgc ccgtcgccac gagctctcgg
     2221 ccctgggcgt tcagagcggc ccggacagcg gtgtctcggc gacgtccata aggcgtggtc
     2281 tcggccgtga catgcacggc aaccgcaccg acctcagtcg ccagggccgg gaccacagtc
     2341 aaaggtgagc cgacccggac gacgagcgac acggcatacc tgtcgcggag gtcggcgtcg
     2401 agcgagcgga gagacgcgag caagcgggcc tgccgcggcg caccagcggc cgcccagagg
     2461 gccgggtcga ggacgaagag cggaaccacg gccccgccgt cggccgcggt gagcagggca
     2521 gggtggtcag cgacgcgcag gtcgcgacgc agccaccaca cgcgtgtgct catcggtgag
     2581 aggctagccc ccgtgaccga catgatcgac accacggaga tgtatctgcg gaccatcttc
     2641 gagctcgagg aagatgggat cccgccgctg cgcgctcgca tcgccgagcg cctggggcat
     2701 tctgggccga cggtgtcgca gacggtcgcg cggatggagc gcgacggcct cctcgccgtg
     2761 accgatcacc gccaccttga gttgtccgag tcgggtcgcc gggccgcgac ccgggtgatg
     2821 cgcaagcacc gcctcgccga gcggctcctc gtcgacgtgc tcgggttgga gtgggagttg
     2881 gcgcacgccg aggcgtgccg gtgggagcac gtcatcagcg atgaggtcga gcggcggatc
     2941 ctggctctcg tcggagattc cacgcgctcg ccctatggca acgccatccc tgggctggcc
     3001 gagctcggcg ggcaggtggg ccgcgacacg acggcggaac gagacagtga ggaacccctc
     3061 actgaggtgc tcggcgtcag tgatcacggc cccatcgcgg gggccaaggc gtccggcgag
     3121 agcgtcgtcg tgcttaccag ccggatcggg gagccggtcc aggtggatca caccgccttg
     3181 tcgctactca cggtggctgg cctcgtgcct ggtcggagcg cgcgagtctc cttgcagggc
     3241 aatgagattc gcgcgatccc tgagggcgtc gccgatcgcg aacccgttgt agtgccgacc
     3301 gaggtcgccg cctacgtgtt cgtcttccgg cgcgcctgag cgggcggtca cgacgatccg
     3361 gtatgccgcc gcgcggcata cctgctgatc gccagccgag gcggtcgtga ccttgtggac
     3421 gccattccgt gaccttggcg tgacattcgg gggtcccgtc ggctaaagtt tgggcactcg
     3481 cccagcaccg ggcgggcccc gaagcggatc gccgagtcct gccaccgccg agggtttgct
     3541 caaaccgcca ggcaggagcg ggggacccac ttagtgggcg tcgtgagacg cccttggggt
     3601 gaagttcgcg gatcggcccc tcaccgggcc gggccgagag ccgggtgatc aactcccacc
     3661 cgaacccgac agctcacctc gtaggcgttc ttggagaggt acatcgtggc aaagcacgtt
     3721 gggcgccacc gcgccccggg gttctccccc ctgaccgagc tgacccagat cgctggcgat
     3781 tccgccaagc ctgccgttcg cgccactgcc gtcatggctg tgtccaccgg catgctcgcg
     3841 ggcatggtcg tgccggcaca ggcggccacc atcgtggccg gcgtggatgg cgtgtcttcg
     3901 gacggtcagg cactcgacac tcccgtcagc gaccttcttg gcattagcgg cctgccgtcg
     3961 accgatggcc cgggcctggc gcctgcggcc ccggccccgg ttgcggccac cggtctggtt
     4021 gaggccgtcg aggctgcggt cggccccgtc gtgaccgctc ccgacaccgt tgcgggcctg
     4081 cccaccttcg gtgcgttggg gttcaagacc aagacgatca ccgtccccgc ggtcacccag
     4141 gtgtcgcgct cgaccgcgcc gcgcacctcg ccggctccgg ccgccaaggc tgcgacgccc
     4201 gccccggccc cggcgcctgt gtccggtggg tcggtcctct cggttgcggc cagcctgctc
     4261 ggcatcccct accgttacgg cggttcttcg ccgtcgaccg gtttcgactg ctctggtttc
     4321 acctcttacg tctatcggat cgcccgtggc atcacgatcc cgcgcacggc cgagggccag
     4381 cgcaagggct cggcgcgcgt cacctccccg gtgcctggcg acctcgtctt cttcggcgtc
     4441 ccggccactc acgtgggcat ctacgccggc aacggcatga tgtacgactc gccgcacacc
     4501 ggtacggtca gccagctgcg caagatctac tccggcaacg tctcgtacgg ccggttctga
     4561 tcgaggcgaa ctaggcccag agacaacgaa aggccccgcc cgggttgccg ggcggggcct
     4621 ttcgctctgg ttgggtcagc tggcccgacg cgacgagcgg ttgacgtcgg ccaacttgct
     4681 ctgcaacacg ccgcgcatca gctcggcttc ggggccctcg tgccggtcgg cggcggcgag
     4741 gaggtcgagg atgtcgccgc ggctctggta gccctcaagc tcgcggaaca tctgggcacg
     4801 gatcgcgcgg gcctcgcggc gctcgcggag gtcatcgcgg acgctggccc agaacccgct
     4861 gcgctcggtg gtggtggtcg gggtggtggt cgttgaagtg ctcatggcct caatggtgcc
     4921 ccgactagat tcatacgtcc aatgtgagga tgctattgaa tccatgcgct agcgtcatga
     4981 gtcatggaca ctgaggtgct gcgctggttc caacaggtcg ccgacggggc aaccgtcacg
     5041 gaggtggctg atctctggat ggtcagtcag cccaaggtct cgcgcgacct catggccctg
     5101 cagcgtgagg tgggggcgcc gttgctcgtc aagtccgggc gggtgctgcg cctcacccac
     5161 gccggtgccg tcttcaagcg ccacgccgac gccatcgtca accggttgga cgacgctctc
     5221 gcggccgtcg accaactcgt cgacccggag cgaggcaccg tcaccctcgc ctacgagcgg
     5281 accctgggca cctggttggt gccgtccttg gtcggggagt tcctcgcggt gcatcctcag
     5341 gtgtcgttca cgctcgttgt cggcggtgag gatctggccg tgctcgatga gggccgggtc
     5401 gatctcgctg tgctcgccag ccgacccaac gcaccgctgt tcggcggggc gagagagggg
     5461 ggtggggcga gcgaggcgag cgtgcgttgg gagccgttgt ttcgcgagcc gttgttcctc
     5521 gcggtcggcc ccggccaccg gttcgaggac atcgagacgg tgccgctggc cgagttggcc
     5581 gacgagccgt gggtggtcct gccgccgggc acggtgatcc gctcggccct cgaccagttg
     5641 tgccacgggg ccggcttcga gccgaaggtc gccttcgaat gcgacgacct cgccacggtg
     5701 cgcggcttcg tcggagcggg cttgggcgtc tcgatcgtcc ccgcaatggg ggaggacccc
     5761 ctgcctcggt atgccgccgc gcctcgcctt gtcgcgctcg ccgacgccgg ggcccgtcgc
     5821 gaagtcggtc tcgcctggtc gtcccagcgg cggttgctcc cggcggccga gttgttccga
     5881 acctttgtcc tgcgttcgtc aggggcgggt cggaccatcg catcatgagg ccgggcgggt
     5941 ctgcaacgat gctccgatgg atcccctaaa cctcatcgag accgccccgg atgatgtcgc
     6001 cttgacgatg tataccggcg agttggtcgt cgatgccatg gtgcgcaaac tcggtcacga
     6061 gccgaatggc accttctggg acggcgtggc cgaatggctc gtcgagaccg aggcgcccac
     6121 gctggaaggg cgattcgact acgacagtga aggcgggatg ttctgcgcct acggcgaccg
     6181 cgccgacctc gaagcgctgc gggccctgat gctccccgtc gtcacggacc ccaagcgggt
     6241 ccggtcactc atcaaggccg ccgaggacgc cgacttcgag ttcgacgact gacggcgcct
     6301 cggcggaaac tacggcaacg gcggcagcgg caacggcggc aacggcatcg tgcgacggac
     6361 agtgggcgac cggcgaccgg cgaccggcga ccggcgaccg gggactgggg gcccggggct
     6421 ggggaccggc atacgcctct gtcgcacgag cgcagagcgg gttaggtgac gtcgagggac
     6481 gaggcctaag cgacgcctga gtcgaaccac ggatctgcct cggggttggc gtcgtgccgt
     6541 ggcgggtgct ctgtcgactc cgacgctcgc cgaggggtgt cgtcccacag gtctccccag
     6601 ggatctgcct cggggttggc gtggtcctgg tcccgggcag agtgatccgt cgccggtggc
     6661 tcgtccggct cccgggagtc ggcccagggg tctgcttcgg gaggtgcggt gtcccgatcc
     6721 gggacagggt cgtccggcgg ccgctgatcg tgcgggtgtt cggcccggta ccaggggtct
     6781 gcttcggggt tggtgtggtc tcggtcgcgg gcagagtgat ccgtcgaggg tggctcgtcc
     6841 gggtctcggg agccagccca cagatctccc cagggatctg cggcggatgg gggctgtgcc
     6901 aacgtgaagg tgcccggtgg atcgccgggt ggttgtccac ccggtggtgg atcgccggat
     6961 ggtgggatcg cgttgtggcg ccggtcgtag ctgccgggga tgacgttcca ggtgacagcc
     7021 ccagtgg
//
LOCUS       DAOMZY010000179         7011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_10097_length_7011_cov_8.864865, whole genome shotgun sequence.
ACCESSION   DAOMZY010000179 DAOMZY010000000
VERSION     DAOMZY010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 7011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7011
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10097_length_7011_cov_8.864865"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..822)
                     /locus_tag="PLK64_10555"
                     /pseudo
     CDS             complement(175..822)
                     /locus_tag="PLK64_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(1117..2721)
                     /locus_tag="PLK64_10560"
     CDS             complement(1117..2721)
                     /locus_tag="PLK64_10560"
                     /inference="COORDINATES: protein motif:HMM:NF023218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPV80232.1"
                     /translation="MAPLPSALGTRRPAAGVGGWYSSQVDALLRDSGAWWYHTWAPHH
                     DWITTPEGCEFVPTIARRTDVTAARLQQATTHGGTLLGFDKPDKSGANSLSPTAALSL
                     WPQLEGTGLRLGAPAVSSNAQARRGWLDSFMSGAVKQRRRVDFIPLHWYPSPSALGSS
                     DPVGAAVNELRQYLQAVHGRFGLPLWLTELSVVAWGSSGQPFVPQPAVQAAFLAEADS
                     MMTALPNVERWAWFSLTPFSGGGSAPLYDDSGSVTQVGDRFRTISAAGAAKEDPAPGS
                     ATAPGVGAWYTSKVDTLLRDSGTPWYYTWAPHHDWITTPAGCEFVPMIWGTRDVNPTG
                     LQQAKANGTTLLGFNEPDESWQDNSLSPAAALSLWPQLEATGLRLGAPGVATRGHVYG
                     GWLDSFMSGLAKQKGRVDFIPLHWYSSPALLTSSKNVVDAAVNDLGQYLEAVHQRYGL
                     PLWLTEFALIGWTADLKNNIVPAQTVQADFLAAAASMMDGLSYVERWAWFSLTQYDMS
                     PGASLYDANNKATLAGERFRSIAAVK"
     gene            complement(2905..3918)
                     /gene="galE"
                     /locus_tag="PLK64_10565"
     CDS             complement(2905..3918)
                     /gene="galE"
                     /locus_tag="PLK64_10565"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003797858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPV80233.1"
                     /translation="MNVLVTGGAGFIGSHTCVELIAAGHDVVVVDDLSAGYVEAVDRL
                     GEITGTRPILEVGSIADAAFVDSVLARHRVDAIIHFAARKAVGESTEIPLEYFHTNVT
                     GTVTLLRAAHRAGVGRVVFSSSCSIYGDTRAVTLDETAPPAPANPYAWTKFACEQLIE
                     QACHYLDGMRAVSLRYFNPIGAHPSGRLGEQPRGVPRNVVPYLAEVATGQRTELQVFG
                     GDYPTPDGTAIRDYVHVVDIAKGHVAALDHVDDTDGMALFNLGTGEGTSVLQLIRSFE
                     AAAGRPIAYEVVARRPGDVPRLVADISAVQAAWGWTPEYDLTAMCEDAWRFVLSNPGG
                     YSP"
     gene            complement(3971..5794)
                     /locus_tag="PLK64_10570"
     CDS             complement(3971..5794)
                     /locus_tag="PLK64_10570"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPV80234.1"
                     /translation="MPAPSRRDPEGIRQYLNELSGGCVVDVRASGPVFGALPSRRPGT
                     RRVLVPALTRRSHRVLLGLTFAFVVTLTAFFVWWVSPVHRLSWPGFVANSVLLAIVLA
                     SPLYFLYIVNQMRRVNPNVPVPDVRVAMVVTKAPSEPWPMVQATLEAMLAQDYPGDFD
                     VWLADEDPADETIDWCYSRGVRISTRQGAKGYQRLTWPRRRRCKEGNLAYFYDHYGYA
                     AYDVVSQLDADHVPDRHYLREMVRPFWDDAVGYVAAPSMCDANGEQSWAVMGRPYEEA
                     FFHGALQLGLSADGTPVCIGSHYAVRTAALRQIGGVGPELAEDFTTSYLLNVAGWRGE
                     YAIDAAARGDGPPNFAAMVTQEFQWSRSLTKALMNLVLRTWTRLPVALGLRFIFQSGF
                     NVLLSLMFVGGVVLFSMASATGSPWVSVNILVFFGLWAAMSGWLLLIAVLLRRSGLNR
                     PVDSPIVSWQRGLYAGSRLPFILLGVVAALVESVVPRSIDFKVTPKGDVGPQPFPVRL
                     VLPFAAVAVILAGSAWMGLDSPDVIGYVGLSLLTALSQVVVATAVTVLHAVETRRNTG
                     IGRPQAMRLVGAPLIVCVLVGAAVSTVSYLYAIELWRFLAA"
     gene            complement(5826..6782)
                     /locus_tag="PLK64_10575"
     CDS             complement(5826..6782)
                     /locus_tag="PLK64_10575"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPV80235.1"
                     /translation="MRVVVTGGAGFVGSHLCDALHARGDHVVCVDDFSSGRFANIEQL
                     RQSPKFELVEQDVRTTIRVTGAVDAVVNMASLASPPAYLARPIFTLQTGSTGTQKALD
                     LAMGHDARFVQASTSEVYGDPLVHPQSEDYWGNVNPVGPRSVYDEAKRYAEALIAAHG
                     RAAGLNGGIIRIFNTYGPRLGLDDGRVVTQFITQALEGAPLTVYGDGTQTRSLCYVSD
                     LVRGFLAMIDGSIAGPVNLGNPHELTVREIAELVIDLVGSDSTIEFHDIPEDDPKRRK
                     PDISMAQAELGWSPQVDVVDGLSRTIEWIRSEGLREDLRTAV"
BASE COUNT         1226 a         2308 c         2365 g         1112 t
ORIGIN      
        1 catcgtcatc accctgggcg acgccttcgt cctcaaccga cgcgtccgtc aacacctgga
       61 atccgccaag cattcgtcga ccccagtgat ggcggcctat gacagcagaa tccgtgacac
      121 tagtggcccg tccgaggttc atcgtcatcc ggcacctcgc gaccgcgcga agctctagaa
      181 gttgatcatg tggccggcga tgccctcgac ggcctcctcg acgctctcgg acagcgtcgg
      241 gtgcgcgaag acgttgcggg cgacctcgtc agcggtgagg tcccacttct gcgcgagggt
      301 gagcaccggg agcagctcgg tgacgtccgg gcctatcatg tgggcgccaa gaatctcgtt
      361 gtgctcggcg tcagcaacga tcttgacgaa gccggcggtg tcgccaagcc cggcggcctt
      421 accgttcgcg gcgaagggga acgtcgccgt cttcacgtcg tagcccttct ccttggcctg
      481 cgcctcggag tacccgaagg aaccgatctg cgggtggcag taggtcgcgc gtgggatgaa
      541 gtcgaagtcg atcggcatcg tgtcggcgcc ggcgatagtc tcggcggcca cgacgcccat
      601 cgcctcggcg gtgtgggcga gcatgagctt ggcggtgacg tcaccgatcg cgtagacgcc
      661 atcgacgttg gtgcgcatgt actcgtcgat cttgatggca ccacggtcgg taagctcgac
      721 gccggtgttc tccaggccat agccctcggt gcggggggcg aagccgatgg cggacatgaa
      781 cttgtcggcc tcgagcgtct gctggtcgcc gcctgaagcc gggggagctg atgcaggaca
      841 tggcgcttac cttccagcag tgtggttccc tcgaaaacgt ggagggcttc cgggcaacca
      901 catgactcct atcatcccct tccaggattg agagcctcca ccaaacccgg ggcggttcac
      961 agttccgctg cgatgttgtc acccatcgcc gacgacgact gttgccctca ccccgagagc
     1021 cgctcagcgg aggcccacca cacttctggg aaaacgcgcc gaggaatcac cctcgtacac
     1081 cacgcccgtg gacttgacca ggatcggtcg gcagatctac ttcacagcag ctatggagcg
     1141 gaaccgctcc cccgcaaggg tggccttgtt gttcgcgtcg tagagcgagg ctcccggcga
     1201 catgtcgtac tgagtcagcg agaaccacgc ccagcgttcc acgtaggaga gtccgtccat
     1261 catcgacgca gcggcagcca ggaagtcggc ctggaccgtc tgggctggca cgatgttgtt
     1321 cttcaagtcc gccgtccaac cgatgagagc gaactcggtg agccagagcg gcaggccgta
     1381 gcgctgatgc accgcctcca ggtactggcc cagatcgttg actgccgcat cgacaacgtt
     1441 cttgctgctc gtgagcagag ccgggctcga gtaccagtgg agggggatga agtcgacgcg
     1501 gcccttctgc ttggccaggc cggacatgaa cgagtccagc cagccgccgt agacatggcc
     1561 acgggtggcc accccgggtg cccccagcct cagcccggtc gcctcgagct gcggccatag
     1621 ggacagggcg gcagccggcg agagggagtt gtcctgccag ctctcgtccg gctcgttgaa
     1681 gcccagcagg gtcgtgccgt tcgccttggc ctgctgcaga ccggtggggt tgacgtccct
     1741 cgtgccccag atcatcggga cgaactcgca cccagccggc gttgtgatcc agtcatggtg
     1801 cggtgcccag gtgtaatacc acggcgtgcc agagtcacga aggagcgtgt cgaccttgct
     1861 cgtgtaccag gcgcccaccc ctggcgcggt ggcgctgcca ggagcagggt cctccttggc
     1921 ggcaccggcg gcggagatcg tgcggaaccg gtcgccgacc tgcgtgaccg agccgctgtc
     1981 gtcatagagc ggcgcactcc cgccacccga gaacggcgtg agcgagaacc aagcccagcg
     2041 ctccacattc ggcaaagccg tcatcatcga gtccgcctcg gcgagaaacg cggcctggac
     2101 cgccggctgg ggtacgaatg gctgcccact gctgccccag gccaccacgc tgagctcggt
     2161 cagccagagc ggcagcccga accggccgtg gaccgcctgc aggtactgcc tcagctcgtt
     2221 gaccgccgcg ccgaccgggt cgctgctgcc gagggctgac gggcttggat accagtggag
     2281 cgggatgaag tccacgcgcc ggcgctgctt caccgcgccg gacatgaagg agtccagcca
     2341 gccacggcgc gcctgtgcgt tgctcgacac ggcgggagca cccagtcgca gcccggtccc
     2401 ctccagctgc ggccacaggg agagcgccgc ggtcggcgag aggctgttcg ccccgctctt
     2461 gtccggcttg tcgaacccga gcagggtccc gccgtgggtc gtggcctgtt gcagtcgggc
     2521 cgcggtgaca tccgtccgac gcgcgatcgt cgggacgaac tcgcagccct ccggtgtcgt
     2581 gatccagtca tggtgcggtg cccacgtgtg gtaccaccag gcgcccgagt cacgaagcag
     2641 tgcgtccacc tggctgctgt accagccgcc gacccccgca gccggtcgcc gagtgccgag
     2701 cgcggatggc aggggcgcca tcggagacac cggactggcc gacgcgcccg ctgcgccgct
     2761 ggacaggaat gtcatcgata gtgtgccgct cagcgcctgc cgtcgagata gcacgcaata
     2821 ctccggtctc tcagccttgc ccaccgagcc gaagaactgg cccctggggt gtgtgtgcct
     2881 gaaacctact gcagaccaaa cggactacgg cgaatagcca ccagggttac tcaagacgaa
     2941 ccgccaggcg tcctcacaca tcgcggtgag gtcgtactcg ggggtccatc cccaggccgc
     3001 ctgcaccgca ctgatgtcgg cgacgaggcg aggcacgtcc ccgggccggc gcgcgacgac
     3061 ctcgtaggcg atgggtcgcc ctgcggccgc ctcgaaggat cggatcagct gaaggaccga
     3121 cgtcccttcg cccgtgccca ggttgaacag tgccatgccg tcggtgtcgt cgacatggtc
     3181 cagggcggcg acgtgaccct tggcgatgtc gacgacatgc acgtagtcac ggatcgcggt
     3241 gccgtccggg gtggggtagt cacctccaaa cacctggagc tccgtgcgct gccccgtcgc
     3301 gacctccgct aggtaaggca cgacattccg ggggacgccc cgcggctgct caccgagccg
     3361 accactggga tgtgcgccga tggggttgaa gtaccgcagg ctgacggcac gcataccgtc
     3421 caggtaatgg caggcctgtt cgatgagctg ctcacaggcg aacttggtcc aggcatacgg
     3481 gttggcgggg gcgggcggcg cggtttcatc caacgtgacc gcacgtgtgt cgccgtatat
     3541 cgaacaggac gatgagaaca ccacccggcc cactccggca cgatgggctg cccgcagcag
     3601 ggtcaccgtg cccgtcacat tggtgtggaa gtactcgagc ggaatctcgg tcgactcccc
     3661 caccgccttg cgggcggcga aatggatgat cgcgtccacg cgatggcgcg ctaggactga
     3721 atccacgaaa gccgcgtccg cgatgctgcc gacctcgagg atcggacggg tgccggtgat
     3781 ttcgcccagc cggtcaactg cctccacata gccggccgag agatcgtcca cgacgaccac
     3841 gtcgtggccg gcggcgatga gttcgacgca ggtgtggctg ccgatgaagc cggcgccgcc
     3901 ggtgacgagg acgttcattg ggttcctcaa ggagggtcgg gtacgagaag agagggtggt
     3961 gctccgggcg ctaggcggcc aggaaacgcc acagctcgat cgcgtacagg tacgagacgg
     4021 tggacacggc cgctccgacc agcacacaga caatgagagg tgcgccgacc agcctcatgg
     4081 cctggggtcg tccgatcccc gtgttccgtc gggtctcgac tgcatgcagg acggtgaccg
     4141 cggtcgcgac cacaacctgc gagagagccg tgagcaagga gaggccgacg tagccgatga
     4201 cgtccgggct gtcgaggccc atccatgcgc ttcccgcgag aatgacggcc acggcggcaa
     4261 aggggaggac cagtcgcacc gggaagggct gcggtcccac gtcacccttt ggagtcacct
     4321 tgaagtcgat ggacctcggg acgacgctct cgacgagcgc ggcgacgacg ccgaggagga
     4381 tgaacggcag gcgactcccc gcatacaggc ctcgctgcca gctcacgatg ggagagtcga
     4441 ccggcctgtt gagtccgctg cgacgcagga gaaccgcgat gaggaggagc cacccgctca
     4501 tggcggccca cagcccgaag aagacgagga tgttgaccga cacccacggc gacccggttg
     4561 cgctcgccat ggagaagagg acgactccgc cgacgaacat caggctcagc aggacgttga
     4621 agccggactg gaagatgaac cgcaggccaa gggcgacggg caacctggtc caggtgcgga
     4681 gcacgaggtt catcagggcc ttggtgaggc ttcgcgacca ctggaactcc tgggtcacca
     4741 tcgccgcgaa gttcggcggt ccgtcgccgc gtgcagcggc gtcgatggcg tactcccccc
     4801 gccacccggc gacgttgagg agataggagg tcgtgaagtc ctcggcaagc tcgggtccga
     4861 ccccaccgat ctggcgcagg gcagcggtgc ggaccgcata gtgcgagccg atgcagacgg
     4921 gcgtcccatc ggcgctgagc ccgagctgca gggcaccgtg gaagaacgcc tcctcgtagg
     4981 gccgacccat gaccgcccac gactgctcgc cgttggcgtc acacatgctc ggcgcggcga
     5041 cgtagccgac cgcgtcgtcc cagaacgggc ggaccatctc gcggaggtag tgacggtcgg
     5101 ggacgtggtc cgcgtcgagc tgggacacca cgtcatacgc ggcatacccg tagtggtcgt
     5161 agaagtaggc gaggttgccc tccttgcagc gccggcgccg cggccaggtc agccgctgat
     5221 atcctttggc gccctgccgg gtggagatcc gcacgccccg gctgtagcac cagtcgatgg
     5281 tctcgtccgc ggggtcctcg tcggcgagcc agacgtcgaa gtcgcccggg tagtcctggg
     5341 cgagcatcgc ctcgagggtg gcctggacca tgggccaggg ctccgacggt gccttggtca
     5401 cgaccatggc gacccggacg tctggcaccg gcacgttggg gttgacccgt cgcatctggt
     5461 tgacgatgta caagaagtat agcggactgg ccagcacgat ggcgagcagc accgagttgg
     5521 cgacgaagcc tggccaggac agccgatgga ccggcgagac ccaccacacg aagaacgccg
     5581 tgagcgtgac gacgaaggcg aaggtgaggc cgagcaggac acgatgcgac cgtcgcgtta
     5641 gagccggcac cagcacgcgc cgggtcccag ggcggcgcga agggagagca ccgaaaaccg
     5701 gcccggaggc ccgcacgtcg acgacgcacc cgcccgagag ctcgttgagg tactggcgga
     5761 tgccctccgg gtctctgcgc gagggcgcgg gcaccccgcc caggccctcg tctctgctcc
     5821 ggacgctaga cagctgttct gaggtcttca cgcagtcctt cggagcggat ccattcgatc
     5881 gtcctcgaca gcccgtcgac gacgtcgacc tgcgggctcc atccgagctc ggcctgggcc
     5941 attgagatgt cgggcttgcg tcgcttcggg tcatcctcgg ggatgtcgtg gaactcgatc
     6001 gtggagtccg agcccacgag gtcgatgacg agttcggcga tctcccggac ggtgagctca
     6061 tgggggttgc cgaggttcac cgggcccgcg atgctcccgt cgatcatcgc taggaagcca
     6121 cgcacgaggt cggagacgta gcacaggctg cgcgtctggg tgccgtcccc gtaaacggtc
     6181 agcggggcac cttcgagcgc ctgggtgatg aactgcgtga cgacccgacc gtcgtcgaga
     6241 cccagtctcg gcccgtaggt gttgaagatc cggatgatcc caccgttgag cccggctgcc
     6301 cggccgtggg ccgcgatgag ggcctcggca tagcgcttgg cctcgtcata gacactgcgc
     6361 gggccgacgg ggttgacgtt gccccagtag tcctcggact gcgggtgcac gagggggtcc
     6421 ccgtagacct cgctcgtgga cgcctggacg aaacgggcgt catgccccat tgccaggtcc
     6481 agcgccttct gtgtccccgt cgaccccgtc tgcaaggtga agatcgggcg cgcgagatag
     6541 gcaggcgggg aggcgaggct cgccatgttg accaccgcgt ccacagcacc ggtgactctg
     6601 atggtggtcc ggacgtcctg ctcgacgagc tcgaacttcg gggattggcg gagctgctcg
     6661 atgtttgcga accggccgga cgagaagtcg tctacacaga caacgtgatc ccccctggca
     6721 tgcaacgcat cacacaaatg cgatccgacg aacccggctc ctccggtgac gactactcgc
     6781 acttaaacca caccccaaga attggtaaac gacccctgcc gctgcggatg acgataaccc
     6841 atgctggggg gtgctccaaa cccgatcggg cccgacgaga caaatgaggg atgtgtccac
     6901 tacccatttc tgcccgcccc ctgctcgtct aggctacgcc tccgatagcg tactgacctc
     6961 ggtctttcat gagggcccgc cgtccgcggc gttgagcttg tcggggtgat c
//
LOCUS       DAOMZY010000180         6989 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_10136_length_6989_cov_5.776320, whole genome shotgun sequence.
ACCESSION   DAOMZY010000180 DAOMZY010000000
VERSION     DAOMZY010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6989
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10136_length_6989_cov_5.776320"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PLK64_10580"
     CDS             complement(<1..605)
                     /locus_tag="PLK64_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HPV80236.1"
                     /translation="MTASPEARPTRVRRQPVEMWFDPMCPWAWMTSRWLMQVETVRPV
                     RVTWSVMSLAVLNEGRDLPEQYRALMEHAWGPVRVIIAAQAAHGDKVIKKLYDALGTR
                     IHLQAREDFDQVIAEALAECKLPAELAKAAHQKKYDAALKRSHKRAISLVGTDVGTPV
                     ISVGGVAFFGPVVSPAPKGEAAGRLWDGCVLVAGTPGFFEL"
     gene            757..3324
                     /gene="pepN"
                     /locus_tag="PLK64_10585"
     CDS             757..3324
                     /gene="pepN"
                     /locus_tag="PLK64_10585"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017884961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HPV80237.1"
                     /translation="MPGTNLTRDEAASRAALLHVNSYDVVLDLTTGPTTFLTSTTVRF
                     AARAAGVGTWIDFVGDSVTSVTLNGVALDPAEVFADSRIALPALAPDNVLVVDAVGRY
                     TNSGEGLHRFVDPVDNEVYLYSQFEVPDSRRVYAVFEQPDLKATFAFTATVPDHWVVV
                     SNQPAPEPVPGGEGRATWAFEPTPRISSYITALCAGPYTVRWDEVQGRDAVIPMGIFA
                     RRSITEHVDYGNLFDCTKAGILFFEKEFDGAFPFAKYDQLFVPEYNAGAMENAGCVTI
                     TDAYVFRGQPSEPLVERRALTILHELAHMWFGDLVTMKWWDDLWLNESFAEWASSVCQ
                     AEATEWPDAWTTFHSHEKTWAYQQDQQSDTHPVYADMRDLVDVTVNFDGITYAKGGSV
                     LKQLVAYVGRDAFREGLRSYFRKHAWGNTTMGDLFAEFEAGSGRDLSDWAQRWLKTSG
                     PSTLALDITTDDNGVITAAAMTQTVAGSDPTLRPHRVGIGRYDLLGGRLDRVGYVEVD
                     VDGPRTELPELVGLPRPALLLPNDEDLTYAKIALDQLSLQTAMAHIDAFDDSLARSLV
                     LGSLWEMLRDGALAPSDFADVLLRVIPVEEHTVVLRTILRTTKQIPSMLLATLRWFLP
                     EAARPAMVDKSVNVIRTALDAAVPGSDKQQQLVVAYAALARHTDDLTRLQGILDGSVV
                     VPGLSVDQDLRWALLTQIAASGGASEDDIAAELDRDPSLAGREQALKARAAIPTAQAK
                     TAAWALAIEQDTQTNAALEAIGAGFRRCDDPALLRPFVESYLDMLEPVFADRSYAIAE
                     RCVKYFYPLDIADAALRDRTRAWLADHEDAPAGLRRLVIEQLAVVETAVAIQEREGS"
     gene            3321..4244
                     /locus_tag="PLK64_10590"
     CDS             3321..4244
                     /locus_tag="PLK64_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HPV80238.1"
                     /translation="MTAYPLLVSPAAWQVTLDQLLGTPLQIFGIVLTAVIGRWLLHRL
                     IRTVVNRATAQHSDRLRILRGRAGEALADASGLADERYVQRTQTVGAVLRSVVTIVIA
                     TLAVLTIMATLGVPLAPLLASAGVGGVALGFGAQSLVRDFLSGIFMIVEDQYGVGDTI
                     DTGEVIGTVEDVSLRVTRLRDRNGVIWYVRNGEILRVGNHSQGWSIAAIAVPFALDAD
                     IAKVTQVLTAALEGLPDRDDRVIEDPSVVIESMSAGTITVRVSARCVPTVNLDVQRDL
                     RVQVKEALDAAGISMAAPVVVPSASPTTGTG"
     gene            4249..4686
                     /locus_tag="PLK64_10595"
     CDS             4249..4686
                     /locus_tag="PLK64_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HPV80239.1"
                     /translation="MSDNVQGVPEGQAPLGRETSFYDLVGGHDTFVRLVHGFYEGVAG
                     DPVLRALYPEEDLGPAEIRLRMFLEQYWGGPNTYSEERGHPRLRMRHTPYAVTPEQRD
                     RWLHHMLASVALLKLDREQEATLVDYLVRAAYMLVNAPDPEKD"
     gene            4729..6333
                     /locus_tag="PLK64_10600"
     CDS             4729..6333
                     /locus_tag="PLK64_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HPV80240.1"
                     /translation="MIYQVYPRSFSDSDGDGTGDLPGVTARLPHLADLGVDGLWLSPF
                     YRSPLADAGYDVADYRDVDPRFGTLADVDTLIARAHELGLAVLVDLVPNHSSDQHPWF
                     QAALAAGPGSPERARYLFRDGRGANGELPPNNWPSVFGGQGWTRVTEADGRPGQWYLH
                     LFDVHQPDFDWTAPEVRAEFESVLRFWLDRGVAGFRVDVAHGLIKAPGLPDLSAPLDE
                     LELLDTNATPFWDQDGVHEVYAAWRGVLDSYGSPERVLCAEAWVSPPERMARYVRSDE
                     MHQSFAFDFLGCRWDAKALRKVIDAELQTMGAVGAPITWVLSNHDVVRHASRLGTADP
                     GPVVGGIRATDPQPDRALGLRRARAATLLMLALPGAAYLYQGEELGLPEHTTLPDAVR
                     DDPVWLRSEHTHAGRDGCRVPLPWVADAPGFGFSPTGASWLPQPAAFAEYAADVQTGV
                     PGSTLELYRETLALRREHRLGRGRLTWVADAPAGTLEFDNGDVRVIVNCSAPAVALPS
                     DRVVAAQSGPLTADGMLPINTAVWLV"
     gene            6422..6724
                     /locus_tag="PLK64_10605"
     CDS             6422..6724
                     /locus_tag="PLK64_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80241.1"
                     /translation="MLVHVEPDDLAAAGYRMSGDGPRLRALAQLISGCGVAGRRACTG
                     APALGQALAALGERGSAAISTLAVATDTLAEGLGDAGRRYGDVEDRLAVMGRRVIG"
     gene            6721..>6989
                     /locus_tag="PLK64_10610"
     CDS             6721..>6989
                     /locus_tag="PLK64_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80242.1"
                     /translation="MSTRDSALAWPPGEVSEIDRIASQLALVATTVDQTVVAREGGQG
                     WGLARSWSGPAFEAARSEAAVVASRSGELAGTVTPIVAGLQGYAAA"
BASE COUNT         1010 a         2486 c         2392 g         1101 t
ORIGIN      
        1 ttgagctcga agaagcccgg cgtcccggcg accaggacac agccgtccca gaggcggccg
       61 gcggcctcac ccttgggggc cggggagacg accggcccga agaaggcgac gccccccacc
      121 gagatcacag gagttccgac gtcggtgccg acgagcgaga tggcccgctt gtggctgcgc
      181 ttgagggccg cgtcgtactt cttctggtga gccgccttgg ccaactcggc cgggagcttg
      241 cactcggcga gcgcctcggc gatgacctgg tcgaagtcct cgcgggcctg cagatggatc
      301 cgggtgccca aggcgtcgta gagcttcttg atgaccttgt cgccgtgggc tgcctgcgcg
      361 gcgatgatga cccgcaccgg cccccaggcg tgctccatga gcgcgcggta ctgctcaggc
      421 aggtcgcgac cctcgttgag gaccgccagg ctcatcaccg accaggtcac tcgcaccggg
      481 cgaaccgttt cgacctgcat gagccagcgg gaggtcatcc aggcccaggg acacatcggg
      541 tcgaaccaca tctcaacggg ctgacggcgg acccgcgtgg ggcgcgcttc gggactggcg
      601 gtcatagggc gcattgtgtc actcccccgc catccgtccc tgaagtgatc tgcccggccc
      661 gtgggaagat ggctgggcaa accaccgcgg cgcggaccgc tgacccggcc gcgcggcata
      721 cccagcagct tggccaccca cccaaggagc gtccctgtgc ccggcacgaa cctcacccgc
      781 gacgaagccg catcccgcgc tgcgttgctc cacgtcaaca gttacgacgt ggtgctcgac
      841 ctcaccaccg gacccacgac gttcctcacg tcgacgacgg tgcggttcgc tgcgcgcgca
      901 gcgggggtcg gcacgtggat cgatttcgtc ggtgactcgg tgacgtcggt gaccctcaac
      961 ggcgtcgccc tcgacccggc cgaggtcttt gcggacagca ggatcgcgct gccggcgctc
     1021 gcccccgaca acgtcctggt cgtcgacgcg gtggggcgct acaccaacag cggcgaggga
     1081 ctgcaccgct tcgtcgaccc cgtcgacaac gaggtctacc tctacagcca gttcgaggtc
     1141 cccgacagcc gccgggtgta tgccgtgttc gagcagcccg acctcaaggc caccttcgcg
     1201 ttcacggcca cggtccccga ccactgggtc gtcgtctcca accagccggc tcccgagccg
     1261 gtcccgggcg gcgagggtcg ggcgacctgg gcgttcgagc cgacgccgcg gatctcctcc
     1321 tacatcacgg cgctgtgcgc cgggccctac acggtgcgtt gggatgaggt gcagggccgc
     1381 gacgccgtca tccccatggg gatcttcgcg cgtcgctcga tcaccgagca tgtcgactac
     1441 gggaacctat tcgactgcac caaggcggga attctgttct tcgagaagga gttcgacggc
     1501 gccttcccgt tcgcgaagta cgaccagttg ttcgtccccg agtacaacgc cggggccatg
     1561 gagaacgccg gctgcgtgac gatcaccgac gcctacgtct tccgcgggca gccctccgag
     1621 ccgctcgtcg agcgccgggc gctgacgatc ctgcacgagt tggcgcacat gtggttcggc
     1681 gacctcgtga cgatgaagtg gtgggacgac ctgtggctca acgagtcgtt cgccgagtgg
     1741 gcctcgtcgg tctgccaggc cgaggcgacc gagtggccgg acgcgtggac gacgttccac
     1801 agccacgaga agacctgggc ctatcagcag gaccagcaga gcgacaccca ccccgtctat
     1861 gcggacatgc gggacctggt cgacgtgacg gtgaacttcg acgggatcac ctacgccaag
     1921 ggcgggtcgg tcctcaagca gctcgtggcc tacgtcgggc gcgacgcctt ccgcgagggc
     1981 ttgcgcagct acttccgcaa gcatgcctgg ggcaacacga cgatgggtga cctcttcgcc
     2041 gagttcgagg ccggctccgg tcgggacctg tccgactggg cccagcgctg gctcaagacc
     2101 tccggcccca gcacgctcgc actagacatc acgaccgacg acaacggtgt tatcaccgct
     2161 gccgccatga cgcagacggt cgccggctct gacccgacgc tgcgtccgca ccgggtgggt
     2221 atcgggcgct acgacctgct cggcgggcgg ctggaccgcg tcggctacgt cgaggtcgac
     2281 gtcgatggcc cgcgcaccga gctgcctgag ctcgtcgggc tcccccgccc tgcgctgctg
     2341 ctgcccaacg acgaagacct cacctacgcc aagatcgcgc ttgaccagtt gtcgctgcag
     2401 accgcgatgg cgcacatcga cgccttcgat gacagcctcg cccgcagcct cgtcctcggt
     2461 tcgctgtggg agatgctccg cgacggcgcc ctggccccgt cggatttcgc cgacgtgctg
     2521 ctgcgggtca tcccggtcga ggagcacacc gtcgtcctgc gcaccatcct gcgcaccacc
     2581 aagcagatcc cctcgatgct gctggccacc ctgcgctggt tccttcccga agccgcccgt
     2641 ccggcgatgg tcgacaagag cgtcaacgtc atccgcacgg cgctggacgc cgcggtcccc
     2701 ggcagcgaca agcaacagca actcgtcgtc gcgtatgccg ccctcgcgcg gcataccgac
     2761 gacctcaccc ggctgcaggg catcctcgac ggatccgtcg tcgtgcctgg cctgtcggtc
     2821 gaccaggacc tgcgctgggc cctgctcacc cagatcgccg cttccggcgg cgcctctgag
     2881 gacgacatcg ccgccgaact ggaccgcgac ccgtcgctgg ccggtcggga gcaggcgctc
     2941 aaggctcgcg ccgctatccc gacggcgcag gcgaagacgg ccgcgtgggc cttggccatc
     3001 gagcaggaca cccagaccaa cgccgccctc gaggccatcg gcgccggatt ccgccgctgt
     3061 gacgatccgg ccctgctgcg tccgttcgtc gagagctacc tcgacatgct cgagccggtc
     3121 ttcgcggacc gctcctatgc catcgccgag cggtgcgtga agtacttcta cccgctggac
     3181 atcgccgacg cggccctgcg cgaccggacc cgcgcgtggc tggccgacca tgaggatgcc
     3241 cccgccggcc tgcgccgact ggtgatcgag cagctggccg tggtggagac cgcggtggcg
     3301 atccaagaac gcgagggttc gtgacggcat accccttgct cgtcagcccc gccgcgtggc
     3361 aggtgaccct cgaccagttg ctcggcacgc cgctgcagat cttcggcatc gtcctcaccg
     3421 cggtcatcgg tcgctggttg ctgcaccgac tgatccggac ggtcgtcaac cgggccaccg
     3481 cccagcacag cgaccgattg cggatcctgc gcggacgggc gggtgaggcc ctcgccgacg
     3541 ccagcgggct ggcggacgag cgctacgtcc agcggaccca gaccgtcggc gccgtgctgc
     3601 gcagcgtggt caccatcgtc atcgccacct tggcggtcct caccatcatg gcgacgctcg
     3661 gcgtgcccct ggccccgttg ttggcctcgg ccggggtcgg cggggtggcg ctcggtttcg
     3721 gggcgcagag cctggtgcgc gacttcctct cggggatctt catgatcgtc gaggaccaat
     3781 acggcgtcgg ggacaccatc gacaccgggg aggtcatcgg cacggtcgag gacgtcagcc
     3841 tgcgagtcac ccgcctgcgg gatcgcaacg gggtcatctg gtacgtccgc aacggcgaga
     3901 tcctccgggt gggcaaccac tcgcaggggt ggtcgattgc cgcgatcgcg gtgcccttcg
     3961 ccctcgacgc tgacatcgcc aaggtgactc aggtgctcac cgcggccctg gaaggcctgc
     4021 ccgatcgtga tgaccgggtg atcgaggacc cctcggtcgt catcgagtcg atgtcggccg
     4081 gcacgatcac ggtgcgggtc tcggcccgat gcgtgccgac ggtcaacctc gacgtgcagc
     4141 gcgacctccg ggtgcaggtc aaggaggccc tcgacgcggc cggaatctcc atggctgccc
     4201 cagtggtcgt accatcggcg tcgccgacga cggggacagg gtgaacgagt gagcgacaac
     4261 gtgcagggcg tgcccgaggg ccaagcgccg ctgggacgcg agaccagttt ctacgacctc
     4321 gtcggcggac acgacacgtt cgtgcgactc gtccacgggt tctacgaggg cgtcgccggt
     4381 gatccggtgc tgcgggcgct gtatcccgag gaggatctcg gcccggccga gatccggttg
     4441 cggatgtttc ttgagcagta ttggggcggc cccaacacct actccgagga gcgtgggcac
     4501 ccacgcctgc ggatgcgcca cactccgtat gccgtgaccc ccgagcaacg cgatcgctgg
     4561 ctccaccaca tgctcgcctc ggtggcgctt ctgaaactgg accgcgagca ggaggcgacg
     4621 ctggtcgact acctggtgcg ggcggcatac atgctggtga acgctcctga ccccgagaag
     4681 gactgacgtg acgatgcact ccccgcgagc gactggtggc gcgacgcggt gatctaccag
     4741 gtctacccgc gctcgttcag cgactccgac ggcgacggca ccggtgacct tccgggggtg
     4801 acggcgcggc tgccccatct ggccgacctc ggcgtcgacg gtctctggct ctcaccgttc
     4861 taccgctcgc ccctcgccga cgccggttac gacgtggctg actatcgaga cgtcgaccca
     4921 cgcttcggga cgcttgcgga tgtcgacacg ctcatcgctc gggcccacga gctcgggctc
     4981 gcggtgctgg tcgacctcgt gcccaaccac tcgtccgacc aacacccgtg gttccaggcc
     5041 gcgttggccg ccggaccggg gtcgccggag cgtgcgcgtt acctcttccg tgacgggcgc
     5101 ggcgcgaacg gcgaactgcc gccgaacaat tggccgtcgg tgttcggtgg tcaggggtgg
     5161 acccgcgtca ccgaagccga cgggagaccg ggtcagtggt acctgcacct cttcgacgtc
     5221 catcagcccg acttcgactg gaccgcgccg gaggtgcgcg ccgagttcga gtcggtgctg
     5281 cggttctggc tcgatcgcgg ggtggccggc ttccgggtcg acgtcgccca cgggctcatc
     5341 aaggctccgg ggctgcccga cctgagcgct cccctggatg agctcgagtt gctcgacacg
     5401 aacgccaccc ccttctggga ccaggacggc gtgcacgagg tgtatgccgc gtggcggggc
     5461 gtgctcgact cctacgggtc gcccgagcgg gtgctctgcg ccgaagcgtg ggtgagcccg
     5521 ccggagcgga tggcccgcta tgtgcgatcc gacgagatgc accagagctt cgccttcgac
     5581 ttcctcggat gccgttggga tgcaaaggca ttgcgcaaag tcatcgacgc cgagctgcag
     5641 accatgggtg ccgtcggcgc tccgatcacg tgggtgctgt ccaaccacga cgtcgttcgg
     5701 cacgcctccc gcttgggcac ggccgaccca gggccggtcg tcggagggat ccgggcgacc
     5761 gacccgcagc ctgaccgggc gttgggcctg cggcgcgcgc gggcggcaac gctgctcatg
     5821 ctcgcgttgc ctggggcggc atacctctat cagggcgagg agctcggcct gccggagcac
     5881 accaccctcc ccgacgcggt tcgcgacgac ccggtctggc tccgcagcga acacacccac
     5941 gccggccgcg acggctgccg ggtcccgctg ccgtgggtgg ccgatgctcc tggcttcggc
     6001 ttctccccca ccggtgccag ttggctgccg cagccggccg cgtttgccga gtatgccgcc
     6061 gacgtccaga ccggcgtgcc cggctcgacc ctcgagctgt atcgggagac attggcgctg
     6121 cggcgcgagc atcgcctcgg ccggggtcgg ttgacgtggg tggcggatgc tcccgcgggg
     6181 acgctggagt tcgacaacgg cgacgtccgc gtgatcgtca actgcagcgc cccagcggtt
     6241 gccctgccct cggatcgggt cgtggccgcc cagtccgggc cgttgacggc cgacgggatg
     6301 ctgccgatca acacggccgt ctggctggtc tgacggcggg ccgcacggcg gacgaggcgg
     6361 gccgctcgca gcctgtggat aacttccgac gacggtcggc gcgagcgcat agcctgccag
     6421 catgttggtg catgtcgagc ccgatgacct cgctgccgcc ggataccgga tgtctggcga
     6481 cggccctcgg ttgcgcgccc tcgcccagct gatctccggg tgcggcgttg ccggacgccg
     6541 tgcctgcacc ggtgcgcccg cgttgggtca ggccttggcc gccctcggcg agcgcgggtc
     6601 cgccgcgatc agcaccctcg ccgtcgcgac ggacacgttg gccgaggggc tgggtgacgc
     6661 cgggcgccgc tacggcgacg tcgaggaccg gttggcggtc atgggccgac gggtgatcgg
     6721 atgagcaccc gcgacagcgc tttggcgtgg ccccctggcg aggtcagcga gatcgaccgg
     6781 atcgcaagcc aattggcgct cgttgccacg acggtcgacc agaccgtcgt cgcccgcgag
     6841 ggtggccagg ggtgggggct ggcgcgctcc tggagcggcc ccgccttcga ggccgcccgc
     6901 tccgaggccg ccgtcgtcgc ctcccgcagc ggcgagctcg ccggcaccgt gactcccatc
     6961 gtggccggac tgcaggggta tgccgcggc
//
LOCUS       DAOMZY010000181         6955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_10186_length_6955_cov_5.213623, whole genome shotgun sequence.
ACCESSION   DAOMZY010000181 DAOMZY010000000
VERSION     DAOMZY010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6955
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10186_length_6955_cov_5.213623"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(369..1346)
                     /gene="whiA"
                     /locus_tag="PLK64_10615"
     CDS             complement(369..1346)
                     /gene="whiA"
                     /locus_tag="PLK64_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HPV80243.1"
                     /translation="MAMTARVKDELSRLQIHKPCCRKAEVSATIRFAGGLHIVGGQIV
                     IEAELDTANAVRRLRRDIAELYGHRAEVVVMSGGGLRRGSRYVLRVVDDGAILARQTG
                     LVDGRGRPVRGLPPKVVSSSACDAEAAWRGAFLAHGSLTEPGRSSALEVTCPGPEAAL
                     ALVGAARRLGIAAKAREVRGVDRVVIRDGDAIAAMLTKMGAHDAVLAWEERRMRREVR
                     ATANRLANFDDANLRRSARAAVAAGARVERALEILGDEAPDHLKEAGALRLQHKQASL
                     EELGELASPPMTKDAVAGRIRRLLAMADKKAADLGIPSTEASLTPDMLE"
     gene            complement(1514..2452)
                     /gene="yvcK"
                     /locus_tag="PLK64_10620"
     CDS             complement(1514..2452)
                     /gene="yvcK"
                     /locus_tag="PLK64_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HPV80244.1"
                     /translation="MNGPRVVALGGGHGLAASLAALRLVTDRVTAVVTVADDGGSSGR
                     LRDEFNVLPPGDLRMALAALCDDRPWGQIWRDVLQHRFGGDGPLGGHAMGNLLIVALW
                     DQLGDTVAGLDLVARLLDSRGRVLPMAGEPLEIEADVVGADPDRPDDVSVVCGQHRVA
                     STRGRVSSIRLRPAHPQPCPEALAAIDDADWVLLGPGSWFTSVLPHVLVPDVRDALVR
                     TSGKRVLNLNLAVHQGETKGFRAVDHLTSLGRHAPELRLDVVLADPSVVDDADEVAAV
                     ASGLGARLVTSPIAATGRPGQHDALRLAAAYRDLMG"
     gene            complement(2449..3387)
                     /gene="rapZ"
                     /locus_tag="PLK64_10625"
     CDS             complement(2449..3387)
                     /gene="rapZ"
                     /locus_tag="PLK64_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HPV80245.1"
                     /translation="MDEHAPEHVPVEGLRAVPADGGPAPLVVVTGMSGAGRSTVAKAL
                     EDHGWHVVENLPPQLLPDLESLHERARPPADGGPSPRLAVVIDARTRWLSGQDAHLLE
                     QLTSGPHRPVLLFLDATDEALVRRFESVRRPHPLQGNGRLLDAIAAERTLMADLRSEA
                     DVIVDTSGLNVHQLHAKVSLLLDATVGSRVRLAVMSFGFKYGVPLDADFVLDMRFLPN
                     PFWVPELRAKSGIDTDVADYVLGQAGATEFLDRAIALFDPVLAGYQREGRRYVTLAIG
                     CTGGKHRSVAVTEALAARFDPERVVAFVVHRDLGRE"
     gene            complement(3403..5466)
                     /gene="uvrC"
                     /locus_tag="PLK64_10630"
     CDS             complement(3403..5466)
                     /gene="uvrC"
                     /locus_tag="PLK64_10630"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPV80246.1"
                     /translation="MADPSTYRPAPGQIPLDPGVYRFRDASGRVIYVGKAKSLRPRLS
                     SYFQDIAALHPRTATMVRTGASVEWTVVGNEVEALQLEYAWIKEFDPRFNVRYRDDKS
                     YPFLAVTLGEQFPRAQVLRGAKRKGTRYFGPYSHAWAIRETLDLLLRVFPIRTCSTGV
                     FKRAAQSGRPCLLGYLDKCAAPCVGRVTEDEHRAIAEEFCDFMAGNTTRFVRRTERAM
                     HDAAAELEFERAARLRDDLVALTKALEKNAVVLGDATDADVFAIVDDELEAAVQVFHV
                     RGGRVRGQRGWVGEKEHEDLPDLVEHLLQQVYGDQPPDAVPREVLVPVLPSDPGPVAS
                     WLATRRGAAVDLRVPRRGDKRALMETVERNAAQSLARHKLSRAGDLTARSVALQELQE
                     ALDLRDAPLRIECFDVSHVQGTNIVASMVVFEDGLARTGEYRRFLVGGPDPQPDDGPA
                     GNLHADATVDDTAAMREVLTRRFSRLVAAESAAAQEAQEAQEAAEAVALSGSSGEPGG
                     MPLAPGLDPQTGRPRKFAYRPNLVVVDGGLPQVTAAAAALRELGITDLALVGLAKRLE
                     EVWVPGQDFPIILPRTSEGLYLLQRVRDEAHRFAITFHRQRRAKAMTASVLDGIPGLG
                     PSRRAALLARFGSVKRLRAATPEEIQEVPGIGPSLATAIVVALARDTATKPASVNVTT
                     GEILD"
     gene            complement(5580..5990)
                     /locus_tag="PLK64_10635"
     CDS             complement(5580..5990)
                     /locus_tag="PLK64_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HPV80247.1"
                     /translation="MATQPSRRSILQVAGTVGAAAATAGTLAGCGGSSAAPAQTTKAA
                     GSALKAADIPVGGGKVVGQVVVTQPTAGTFKAFSAVCTHEGCLVSKIESAKITCTCHG
                     SVFDAATGAVVSGPAKGPLAAKTVTVSGDSLTLS"
     gene            6232..>6955
                     /locus_tag="PLK64_10640"
     CDS             6232..>6955
                     /locus_tag="PLK64_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HPV80248.1"
                     /translation="MQQRSIVRLGVPLAVAALALSACGSRDAGTSSTAAGTKTVKIGV
                     IAPLSGGLSALGLGIKNSVALAVKQANDSNAIAGWKFEVAAEDDEAKPETGKNAATKL
                     SSDDAVLGVVGTLNSSVAQSTVPVLAAANIAQVSPANTNPSLTQGADAAAKKRPYANY
                     FRTCTTDAVQGPFAAQYLLSVGIKSVATIHDKKTYGQGLVDAFTAEFTKGGGTVVAAE
                     TIGEQDKDFSAVISKIKPSAPAA"
BASE COUNT         1070 a         2439 c         2433 g         1013 t
ORIGIN      
        1 agttacggcc gatgcggccg aagccgttga tgcctacctt gacggtcact gtgatctaaa
       61 ccttccgact ggagtcgatg gacacgcccc cgggctcacc tcgacgacca gcggccgtcg
      121 tcggcagccc gtggtaccgc cccaacccta gtgctcccag cggcccgcgg tctaagcggt
      181 ctcaagacgc aaatcgaggt caggacgtag gtccttgccg ggccgtgacg cacgccaccg
      241 cagggtgagc agcgggtgtc gcgcagatgt ccagaaggtg acgcacggca taccccatgg
      301 ggtatgccgt gctgccgcgg gtcagcgtgc ggcgggtgcg agtcagcgtg cggcgggtgc
      361 ggcgacggtc actcgagcat gtcgggggtg aggctggcct cggtgctcgg gatcccgaga
      421 tcggcggcct tcttgtcagc catcgccaac agccggcgga tccgccccgc gaccgcgtcc
      481 ttggtcatcg gcggcgaggc caactcaccg agctcctcca gcgaggcctg cttgtgttga
      541 agacgcaacg caccggcctc cttgaggtgg tcgggagcct cgtcgccgag gatctccagg
      601 gcacgctcca cccgggctcc ggccgcgacg gcggcgcgcg ccgagcggcg gaggttcgcg
      661 tcgtcgaagt tggccagccg gttggccgtc gcgcgcacct cgcggcgcat ccgacgttct
      721 tcccacgcca gcacggcgtc gtgggcaccc atcttggtga gcatggccgc aatggcgtcg
      781 ccgtcgcgga tgaccactcg gtcgacgccg cgcacctcgc gggccttggc cgcaatgccc
      841 aagcgacggg cggcgccgac cagggccagc gctgcctcgg ggccggggca ggtgacttcg
      901 agtgcgctcg accgtccagg ctcggtcagg gagccgtggg cgaggaaggc cccgcgccag
      961 gcggcttcgg cgtcgcaggc gctggaggag accacctttg ggggcagccc tcgcacgggt
     1021 cgcccacgac cgtcgaccag gccggtctga cgcgccagga tcgccccgtc gtcgacgacg
     1081 cgcagcacgt aacggctccc ccgccgcagg ccgccaccgg acatgacgac gacttcggcc
     1141 cgatggccat agagctcggc gatatccctg cgcagccgac ggacggcgtt ggcggtgtcg
     1201 agctcagctt cgatgacgat ctggcccccg acgatgtgca ggcccccggc gaagcggatc
     1261 gtggccgaga cctcagcctt gcgacagcac ggcttgtgga tctgcagccg ggacagctcg
     1321 tccttcactc gcgccgtcat ggccattgat cgaacctgcc cttgcctcgt gtgtcaccaa
     1381 tcggatcgac ccgcccctgg gtgacgaatg ccgcgtcagc ctagccgatg ggaggttcgc
     1441 gtgcggctca agatgaacat cgatcaacat ctcgggcaac gggttgacgg ggctgcggca
     1501 ccccgcgccc gactcatccc atgaggtccc ggtatgccgc ggcgagacgg agcgcgtcgt
     1561 gctggccggg gcgcccggtg gcggcgatcg gcgaggtcac caaccgcgct cctaagccac
     1621 tggcgaccgc tgccacctcg tcggcatcgt ccacgacgct cgggtcggcc aggacgacgt
     1681 cgaggcgcaa ttcgggggca tgccgcccca acgaggtgag gtggtcgacc gcccggaaac
     1741 ccttcgtctc gccctggtgg acagcgagat tgaggttgag gacccgcttg ccgctcgtcc
     1801 ggaccagcgc atcgcggacg tcgggcacaa ggacatgagg aaggactgag gtgaaccacg
     1861 acccgggccc gaggaggacc caatcggcgt cgtcgatcgc ggccagcgct tcgggacagg
     1921 gctggggatg ggcagggcgc agccgaatgc tcgacacccg gccgcgggtc gatgccacgc
     1981 ggtgctggcc acagacgacg gacacgtcgt cgggtcgatc cgggtcggcc ccgaccacgt
     2041 ccgcctcgat ctccaagggc tcgccggcca tgggcaggac ccgcccccgg gagtccaaca
     2101 gccttgccac gaggtcgagg ccggcgaccg tatcgcccaa ctggtcccag agggcgacga
     2161 tcaacaggtt gcccatcgca tgcccgccca acgggccatc cccaccgaac cggtgttgga
     2221 gcacgtcgcg ccagatctgc ccccacggcc ggtcgtcgca gagagcggca agcgccatcc
     2281 tcaggtcgcc cggcggcagg acgttgaact cgtcgcgcag ccgaccggat gatcccccgt
     2341 cgtcggccac cgtgaccacc gccgtcaccc ggtcggtcac cagacgcagc gccgccaacg
     2401 acgccgccaa accatgcccg ccccccagcg ccaccacccg gggaccgttc attcgcggcc
     2461 gagatcgcga tgcacgacga acgccaccac gcgctccggg tcgaagcgcg cggccaacgc
     2521 ctcggtgacg gcgaccgagc gatgcttacc gccggtgcag ccgatcgcga gggtgacata
     2581 ccgccgtccc tcgcgctgat agccggccag cacggggtcg aacagtgcga tcgcccggtc
     2641 gaggaactcc gttgccccgg cctggccgag cacgtagtcg gcgacgtcgg tgtcgattcc
     2701 ggacttggcc cgcaactccg ggacccagaa cgggttcggc aggaagcgca tgtccaggac
     2761 gaagtccgca tcgaggggga cgccgtactt gaagccgaac gacatgaccg ccaagcgcac
     2821 ccgggacccg acggtggcgt cgaggagcaa ggacaccttg gcgtgcagtt ggtggacgtt
     2881 cagccccgag gtgtcgacga tgacgtccgc ctcagaccgc aggtcggcca tgagggtgcg
     2941 ttcggcggcg atcgcgtcaa ggagtcgccc gttgccctgc aaggggtgtg gtcgtcgcac
     3001 gctctcgaac cggcgcacca acgcctcgtc cgtggcatcg aggaagagca ggaccggtcg
     3061 atgcggaccg gacgtgagct gttcgagcag gtgcgcgtcc tggccggaca gccaccgggt
     3121 ccgggcatcg atgacgacgg ccagccgcgg cgaggggccg ccgtccgccg gaggccgggc
     3181 ccgctcgtgc aacgactcga ggtccggcag caactgcggc gggaggttct cgaccacatg
     3241 ccacccgtgg tcctcgagcg ccttggcgac cgtcgaacgc ccggccccgc tcatgcccgt
     3301 gacgacgacg aggggcgctg ggcctccgtc ggccggcacg gcccgcagcc cctccacagg
     3361 cacatgttcg ggtgcgtgct cgtccatgct cggtcctccc gctcagtcga ggatctctcc
     3421 ggtggtcacg ttaacgctcg caggtttcgt cgcggtgtcg cgggccagcg ccacgacgat
     3481 cgccgtcgcc agggacgggc cgatcccggg cacctcctgg atctcttcgg gcgtggccgc
     3541 ccgcaggcgc ttgaccgagc cgaacctcgc caggagcgcc gcgcgacgcg acggaccaag
     3601 accgggtatg ccgtcgagca ccgatgcggt catcgccttg gcccggcgct gtcggtggaa
     3661 ggtgatggcg aaccggtggg cttcgtcgcg gacccgttgc aggaggtaga gaccctcgct
     3721 cgtgcgcggg aggatgatcg ggaagtcctg tccgggaacc cacacctcct ccagccgctt
     3781 ggccagtccc acgagggcca ggtcggtgat ccccagctcc cgcaaggcgg ctgccgcggc
     3841 ggtgacctgt gggagtcccc cgtccacgac gacgaggttg ggtcggtagg cgaacttgcg
     3901 cgggcgcccg gtctgcgggt cgaggccggg ggcgagcggc ataccccctg gttcaccgga
     3961 cgaccccgac agggccaccg cttcggcggc ctcctgtgcc tcctgagcct cctgagcggc
     4021 agccgactcg gcggccacca gtcgggagaa ccggcgggtg aggacttcgc gcatggcagc
     4081 ggtgtcgtcg accgtggcgt cggcatggag gttcccggct ggcccgtcat cgggctgcgg
     4141 gtcggggcct cccacgagga agcgtcggta ttcgccggtg cgcgccaaac cgtcctcgaa
     4201 gacgaccatc gaagcgacga tgttggtgcc ttgcacgtgt gacacgtcga agcattcgat
     4261 ccgcagtggc gcatcgcgaa ggtcgagcgc ctcctgcaac tcctgtaggg ccacgctgcg
     4321 cgccgtgaga tcaccggcgc gactgagctt gtgtcgggcc agggactgcg cggcgttgcg
     4381 ttcgacggtc tccatcaggg ctcgcttgtc accccggcgc gggacgcgca ggtcgaccgc
     4441 tgcccctcgc cgggtggcga gccacgacgc gaccgggccc gggtcggagg gcaggacggg
     4501 gacgagcacc tcacggggaa cggcgtcggg gggctggtcg ccgtagacct gctgcaggag
     4561 gtgctcgacg aggtcgggaa ggtcttcgtg ttccttctcg cccacccacc cgcgttggcc
     4621 acggactcgc ccgccgcgga catggaagac ctgtacggcc gcttcgagct cgtcgtcgac
     4681 gatggcgaat acgtcggcgt cggtcgcgtc gcccaggacc acggcgttct tctccagcgc
     4741 cttggtgagt gccacgaggt cgtcccgcag ccgagccgcc cgctcgaact ccaactcggc
     4801 ggcggcgtcg tgcatggccc gctcggtgcg acggacgaag cgggtggtgt tgcccgccat
     4861 gaagtcgcag aactcctcgg cgatggcgcg gtgttcgtcc tcggtgaccc gcccgacgca
     4921 gggtgccgcg cacttgtcga ggtagcccag cagacagggg cgtccgctct gggccgcccg
     4981 cttgaacacg ccggtgctgc acgtgcggat cgggaacacg cgcaggagca ggtcgagcgt
     5041 ctcgcggatc gcccaggcat gcgagtaggg cccgaagtag cgggtgccct tgcgcttggc
     5101 gccgcgcagg acctgagctc gggggaactg ctcccccaag gtgaccgcga ggaacgggta
     5161 ggacttgtcg tcgcggtatc ggacgttgaa gcgcggatcg aactccttga tccacgcgta
     5221 ttccagctgc agcgcctcga cctcgttgcc gacgacggtc cactcgacgc tggcgcccgt
     5281 gcggaccatg gtcgccgtgc gggggtgcag ggcggcaatg tcctggaagt aggacgacag
     5341 gcgtgggcgc agcgacttgg ccttgccgac gtagatgacc cgcccggagg cgtcccggaa
     5401 ccgatagacc ccggggtcga gcgggatctg gcccggggcc gggcggtacg tcgatgggtc
     5461 ggccacggcc ccactgtagg aggcgggacc gacggccccc ctgcggcgct cacgcgcggg
     5521 ccatcgtccc ccgatgttcc tgttctggtc ggctctggtc gcggcctccg gccgccgact
     5581 cagctcaggg tcaaggagtc accagagacg gtgaccgtct tcgcggccag ggggcccttc
     5641 gccgggccgc tcacgaccgc tccggttgcg gcgtcgaaca cgctgccgtg gcaggtgcag
     5701 gtgatcttgg cgctctcgat cttgctcacc aggcagccct cgtgggtgca caccgcgctg
     5761 aacgccttga aggtgccggc ggtcggctgg gtgacgacga cctgtccgac gaccttgcca
     5821 ccgccgaccg ggatgtccgc cgccttgagc gcggaacccg cggctttcgt cgtctgcgcg
     5881 ggggcggctg agctgccacc acaaccggca agggtgcccg ccgtggccgc cgcagcgccg
     5941 acagttccgg cgacctgcag gatcgagcga cgactgggtt gagtggccat ctggggtcct
     6001 ccaaggcttg cgtgggtggt cagggaccaa gcatccctgg tatgccgccc cccggccacc
     6061 tgtccgttgg ggcacctgcg ggtgccccaa cggcccatcg gccccataac gacacgggaa
     6121 caacccgaag ccggttgggg acaccgggcc tccggacccg tactctgcac cctagtccgc
     6181 ggtcgtcgcg gagtccggag gatgcgccgg acaagattca caggaggaac tgtgcagcag
     6241 cgttccattg ttcgactggg cgtgccgctt gcggtcgcgg ccctggccct gtccgcgtgc
     6301 ggaagccgcg acgccggcac cagcagcacc gctgctggca ccaagacggt gaagatcggc
     6361 gtcatcgcgc cgttgtccgg tggcctgtcc gccttgggcc tgggcatcaa gaactccgtc
     6421 gcccttgccg tcaagcaggc caacgactcc aacgcgatcg ccggctggaa gttcgaggtg
     6481 gcggccgagg acgacgaggc caagcccgag accggcaaga acgccgccac gaagctctcg
     6541 tccgatgacg ccgttctcgg cgtggtcggc acgctgaact cctcggtggc gcagtcgacc
     6601 gtcccggtcc tggccgcggc gaacatcgcc caggtctcgc ccgccaacac gaacccgagc
     6661 ctcacccagg gtgccgacgc ggcagcgaag aagcggccgt acgccaacta cttccgtacc
     6721 tgcaccactg acgctgtcca gggtccgttc gcggcccagt acctcctcag tgtcggcatc
     6781 aagagcgtgg ccaccatcca cgacaagaag acctacggcc agggcctcgt tgacgccttc
     6841 accgccgagt tcaccaaggg cggcggcacc gtggttgccg ccgagaccat cggcgagcag
     6901 gacaaggact tctcggcggt catctccaag atcaagccgt cggctcctgc cgcga
//
LOCUS       DAOMZY010000182         6941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_10222_length_6941_cov_6.502033, whole genome shotgun sequence.
ACCESSION   DAOMZY010000182 DAOMZY010000000
VERSION     DAOMZY010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6941
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10222_length_6941_cov_6.502033"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            406..585
                     /locus_tag="PLK64_10645"
     CDS             406..585
                     /locus_tag="PLK64_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80249.1"
                     /translation="MTATVEGPAVELLWWLQGDPFPEEIVSVTGVDADVRALKQTFLQ
                     PVSKPPRRWFGLLPG"
     gene            complement(594..1793)
                     /locus_tag="PLK64_10650"
     CDS             complement(594..1793)
                     /locus_tag="PLK64_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HPV80250.1"
                     /translation="MKLDLTVLAIPAYVGAMAAEYAWQRKHPVEPGTRAGDYEFKDTM
                     ASLTMGLGSLVAPLVLKRLQDPVTPGRGKYAKVLMGTALAAGVATTAIDLIRRHRAGG
                     LPDPGTVDWDPRDLGAAVDSDRGAASADASLPPVLQRVGAGAAVAAVASTVVTVSSLW
                     GAETTGRALFRRSPLDLGSGAAATAVAIMGWDFIYYWNHRLDHEVRWLWAMHVVHHSS
                     ERYNLSTALRQPVAESLVLYVPYSLMALAGVRPELLEKARALNLIYQFFVHTEAVKSI
                     GWLERYLNTPSHHRVHHGSNSEYLDRNHGSILILWDKLFGTFEKEDARVVYGLTKNIN
                     SYNPATIATHEWGALFADVARSTSWRDRASFLVRGPGWAHDRRLEQLAAGVADRLPAA
                     SAGATAA"
     gene            1856..2452
                     /locus_tag="PLK64_10655"
     CDS             1856..2452
                     /locus_tag="PLK64_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV80251.1"
                     /translation="MGDIGTKGVPRADRERLLLSAAIEEFGRRGYAGASLTRIASTAG
                     VSKAMVYHLFDSKEAVALAAVEHLGAGLVKAVADAQVSAIPARRALDTLTAIFVALDE
                     NRQVWAIIHDTTLPVGSPAAVAAARYRGELALLGTTGSGAVVSGVGVDDRLDLDLIDR
                     IWQSAVATTMRWWQEHDDVSAAEMAERCARMLAILGSS"
     gene            complement(2511..3284)
                     /locus_tag="PLK64_10660"
     CDS             complement(2511..3284)
                     /locus_tag="PLK64_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPV80252.1"
                     /translation="MSSDIILGIDVGGTGIKGAPVNVSTGEQIGERHRIPTPEDSTPE
                     AVAEVVAAVAEHFADVLMPGSPVGVTFPGVVQHGIVRTAANVDHGWIDHAGEELFGQV
                     LGRPCVLVNDADAAGVAEARFGAAKDSTGFVIVTTLGTGIGVALLHNGVLVPNAELGH
                     IEMHGEVAEKLAAASAKEREDLSYKKWARRLQDYYSRIENLLWPDLFIVGGGKSKNAE
                     KFLPLLDLRTPIVPAMLENSAGIIGAAALAAEREGAGRD"
     gene            complement(3281..5659)
                     /locus_tag="PLK64_10665"
     CDS             complement(3281..5659)
                     /locus_tag="PLK64_10665"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HPV80253.1"
                     /translation="MLDTILGSEDWLLRENGCDLARANAWETLFTTGNGRIGTRGSLE
                     EGHAGALPGVYVNGVFDAHDAIVTDMVNAPDWVGVEVHVEGVRLDVHSCRVVSHERAL
                     DLRQGVLWRSTVFEDPHGRQSRLDTVRFASQVARDTLALVVEVTPLGHDATVEIGTGL
                     DGDRRNLEALPVYPSDRAFTVQGRWEKWARSQHLEAQDTAVTDSGVLQLRSRTIQSDV
                     TLAYAAVTTTAPSPDRAWTSRSSTSVTSRTTQHVSAGSTVRLEKFVVIATSRDHAAPT
                     GDVLDRATERAEAAAAQGFSSLLQDNAAAWRQLWQACDAEVVGDEELTLAVRFSIYQL
                     LIAANPDDPTVNVGAKSLSGEGYRGHVFWDTEVMLLPFYVLTQPATAKALLGYRHFTL
                     DGARRNSTENGTTGARYAWESADTGDEECPRFTPDGKNRFWTRDEEVHVSADVAYGIH
                     RYVEATGDTDFLHTQGADMLFDTARFWASRIDRADGDPRGHLKQVMGPDEFHSHVDDN
                     AFTNRLVQWHLRYAVSVFDAFVAQAPEVLADVSDRLGLGADEVETWRSLAENLAVGEP
                     NEHGVIEQFTGYFDRLDVPVTEWDENSMPKYPPGYNHFNCEDTQLLKQPDVLMLALML
                     PDLFDADSTRANFEYYEPRTLHKSSLSPAIHAIIGIEVGDTTRAQQYFDRSAFVDLRD
                     NQGNTAEGMHIASAAGTWAVLVHGFGGLRVEHGRLSINPWVPTDWQAVRYRITWRGLH
                     LAIEATREEVTITATGESAESLDVTVHGQPVTVAAGSTVTTAAPATVGGSTA"
     gene            complement(5665..6663)
                     /locus_tag="PLK64_10670"
     CDS             complement(5665..6663)
                     /locus_tag="PLK64_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HPV80254.1"
                     /translation="MSARTVAVLGPIPRDRIITHRGDVLEKYGCALYTAIALSALLGE
                     EDRVLPIVHVEKVDEQPIKDLFASYPNVDVTGIRSTTDRGDVVELRYVDQNTRIERQT
                     GFMAPIRPVDVEFALHADAFVCVPITDYEVSQSTLEYIKANSEGVILLDGHGPTSTLV
                     HDGAREHRLWADRDAWLPSIDILKMNLEEAGCSWMPGPGDDRRPGDPIAVDQLPDFAA
                     HVLGQGVKAVCVTLDEEGCVAFTLDDAGALQSHVVPRIPIEDVVDTTGCGDSFAAGMA
                     FGYLQDGDIVRACQYGNAMGAQRASASELDGYLPLAQTDAQLAQTYGGATTAPVGG"
BASE COUNT         1112 a         2289 c         2382 g         1158 t
ORIGIN      
        1 tcccgcggta gcgggtcttg gtgaaggcga agtcacgctt gacgatgagg aacggatgct
       61 cgaccttggc ccgcaccgac gcctgacgcg aggcaacggc ccggtcatgc tcgggcatcg
      121 tcttcaacgc acccttgcgc gccgcgaccc tccacgtgat ccgcgacagg tcatcatggt
      181 cggcgatctc gggccgcttg ggcgctgacc ggccccgttg cccgcgacgg catcgaccac
      241 aacctctcgc attgggcttc atcggacccg agcggcaaga cgtggcaaac ccaagccctt
      301 gccatccggg cacgggacgc cgacgccacc tggcgcatcg tcctccctca ggacaccgaa
      361 ctgcacctgg gcgacttccg catcgcaccc gggcaactcc aggaggtgac cgcgacggtc
      421 gaggggccag cggtcgaact cctgtggtgg ttgcagggag acccattccc cgaggagatc
      481 gtgtcggtca cgggagtcga tgccgacgtc cgggcgctca agcagacctt cctgcagccg
      541 gtgtcgaagc cgcctcgccg ctggttcgga ctccttccag gctgatcccg ggctcaggct
      601 gccgttgcgc ccgccgacgc tgctggcagg cgatcggcca cgcctgcagc caactgctcc
      661 agtcgccggt cgtgggccca gccgggtccg cgcaccagga acgacgcgcg atcgcgccac
      721 gacgtggatc gagcgacatc ggcgaagaga gcgccccact cgtgcgtcgc aatggtcgcc
      781 gggttgtagg agttgatgtt cttggtcagc ccatagacga cgcgagcgtc ttccttctcg
      841 aacgtcccga agagcttgtc ccacaggatg aggatgctgc cgtggttgcg gtcgaggtat
      901 tcgctgttgc tgccgtggtg cacccggtgg tgtgaaggcg tgttgaggta gcgctccaac
      961 caaccgatcg acttgaccgc ctcggtgtgc acgaagaact ggtagatgag gttgagggct
     1021 cgcgccttct cgagcagctc gggccggacg ccagccagtg ccatcagcga gtaggggaca
     1081 tagagcacga gcgactccgc gaccggctga cgcagcgccg tcgagaggtt gtagcgctca
     1141 ctggagtggt ggacgacatg catcgcccac agccagcgca cctcgtgatc gagccggtgg
     1201 ttccagtagt agatgaagtc ccaccccatg atcgccacag ccgtcgccgc cgcaccgctg
     1261 ccgaggtcca ggggcgaccg gcggaagagg gctcgacccg tggtctcggc gccccagagc
     1321 gaggacacgg tcaccaccgt cgatgccacc gcagcaacgg ccgctcccgc gccgacccgc
     1381 tggagcaccg gcgggagcga cgcgtccgcc gaggccgccc cccgatcgga gtcgacggca
     1441 gcaccgaggt cgcgagggtc ccagtccacg gtgcccgggt ccgggaggcc acctgcgcga
     1501 tggcgccgga tcaggtcgat ggcggtcgtc gccacacctg ctgcgagcgc cgtgcccatg
     1561 agcaccttcg cgtacttccc gcgcccggga gtcacggggt cctggagccg cttgagcacg
     1621 agcggagcga cgagactgcc cagccccatc gtcaggctcg ccatcgtgtc cttgaactcg
     1681 tagtcgccgg cgcgcgtgcc cggctccacc ggatgcttgc gctgccacgc gtactctgcc
     1741 gccatggccc cgacataggc ggggatggca aggacggtca ggtcgagctt catgaactga
     1801 ccctatggtc agttccatcc ggtccgcaag cgaatgagat gagagaggca gcacagtggg
     1861 cgacatcggg accaagggcg tgccccgcgc tgaccgcgag cgcctgctcc tcagcgcagc
     1921 gatcgaggag ttcggtcgcc gcggctatgc cggcgcctcc ctcacccgga tcgcctcaac
     1981 cgcaggtgtg tcgaaggcga tggtctatca cctgttcgac tccaaggagg cggtggcgct
     2041 cgcggccgtc gagcatctcg gtgcgggtct ggtgaaggct gtcgccgacg cacaggtgtc
     2101 tgccatcccg gctcgtcgcg cgctcgacac cctcaccgcc atctttgtcg cgctcgatga
     2161 gaaccgtcag gtgtgggcga tcattcacga cacgaccctc cccgtggggt cgcccgcagc
     2221 ggtggcggct gcccgctatc gcggcgagct cgccttgctc ggcaccacgg gcagtggcgc
     2281 cgtggtgtcg ggtgtcggcg tcgacgaccg gctcgacctc gacctcatcg accgcatctg
     2341 gcagtcggcc gttgccacga cgatgcgttg gtggcaggag cacgacgatg tcagcgccgc
     2401 cgagatggca gagcgctgcg cgcggatgct cgcgatcctc ggcagttcct gacgcaggct
     2461 cagatcgcgc tcggcacagg cacattcgcc gggtatgccg tccgctccag tcagtccctg
     2521 cccgcacctt cgcgctcggc cgccagagct gccgcgccga tgatcccggc ggagttctcc
     2581 agcatcgccg gcacgatcgg cgtgcgcagg tcgaggagcg gcaggaactt ctcggcgttc
     2641 ttggacttcc cgccaccgac gatgaagagg tctggccaca gcaggttctc gatgcgggag
     2701 tagtagtcct gcaaccgcct ggcccacttc ttgtaggaca ggtcctctcg ctccttggcg
     2761 ctcgcggcgg cgagcttctc ggcgacctct ccgtgcatct cgatgtgacc caactcggcg
     2821 ttcggcacga ggacaccgtt gtggagcaag gccacgccga tcccggtccc gagggtcgtg
     2881 acgatgacga acccggtcga gtccttggcg gcaccgaagc gcgcctcggc gacacctgcc
     2941 gcgtcggcat cgttgacgag gacgcagggc cgtccgagga cctggccgaa cagttcctcg
     3001 ccggcatggt cgatccaccc gtgatccacg ttggctgcgg tgcggacgat gccgtgctgg
     3061 acgacaccgg ggaaggtcac tcccaccggt gacccgggca tcagcacgtc ggcgaagtgc
     3121 tccgccactg cggcgaccac ctccgcgacg gcctccggag tcgagtcctc gggcgtcggg
     3181 atgcggtgcc gctcgccgat ctgttcgccc gtgctgacgt tgaccggcgc gcccttgatg
     3241 ccggtgccac ccacgtcgat cccgaggatg atgtcgctgc tcatgctgtg cttccaccca
     3301 cggtggccgg ggccgccgtc gtcacggtcg agccggcagc gaccgtgacc ggctggccgt
     3361 gcacggtgac gtcgagggac tctgccgact cgcctgtcgc cgtgatggtg acctcctcgc
     3421 gggtggcctc gatcgccaga tgcagtccgc gccaggtgat ccgatagcgc accgcctgcc
     3481 agtcggtggg cacccacggg ttgatcgaca accggccatg ctcgacccgg agcccgccga
     3541 acccatggac gagcaccgcc cacgtcccag ccgccgaggc gatgtgcatg ccctcggcgg
     3601 tgttgccctg gttgtctcgg aggtcgacga aggccgaacg atcaaagtac tgctgggccc
     3661 gcgtcgtgtc accaacctcg atgccgatga tggcgtggat cgccgggctg agtgaggact
     3721 tgtggagggt cctcggctcg tagtactcga agttcgcccg ggtgctgtcc gcgtcgaaca
     3781 ggtcgggcaa catcagggcg agcatgagca cgtcgggctg tttgagcagc tgcgtgtcct
     3841 cacagttgaa gtggttgtag cccggcggat acttcggcat cgagttctcg tcccactccg
     3901 tcaccggcac gtcgagccgg tcgaaatagc cggtgaactg ctcgatgacg ccgtgctcgt
     3961 tgggctcgcc caccgccagg ttctcggcga gtgagcgcca cgtctcaacc tcgtcggcac
     4021 ccagaccgag ccggtccgaa acgtcagcca acacctcggg tgcctgagcc acgaaagcgt
     4081 cgaagacact cacggcatac cggaggtgcc attgcacgag tcggttggtg aaggcgttgt
     4141 cgtcgacgtg cgagtggaac tcgtccggcc ccatgacctg cttgagatga ccacgagggt
     4201 caccgtcggc gcggtcgatc cggctcgccc agaagcgcgc cgtgtcgaag agcatgtccg
     4261 caccctgcgt gtgcaggaag tcggtgtccc cggtcgcttc gacataccgg tgtatgccgt
     4321 aggcgacatc cgcagagacg tgcacctcct cgtccctggt ccagaaccgg ttcttgccat
     4381 cgggggtgaa gcgcgggcac tcctcgtcgc cggtgtcggc cgactcccag gcgtagcgcg
     4441 cgcccgtcgt tccgttctcg gtcgaattgc ggcgggcgcc gtcgagggtg aagtgccggt
     4501 atccgaggag cgccttggcg gtggcgggct gcgtgaggac atagaacggc agcagcatga
     4561 cctcggtgtc ccagaagacg tgcccgcggt agccctcacc ggacagggac ttggcgccga
     4621 cgttgacggt cgggtcgtcc gggttcgccg cgatgagcag ttgatagatg ctgaagcgga
     4681 cggccagggt cagctcctcg tcaccgacga cctcggcgtc gcaagcctgc cacaactgcc
     4741 gccacgcggc ggcgttgtcc tggaggagtg acgagaaacc ctgtgctgca gcggcttctg
     4801 cccgctcagt ggcccgatcg aggacgtcac cggtgggcgc tgcgtgatcg cgggaggtgg
     4861 cgatcacgac aaacttctcg agccggacgg tggatccggc cgacacgtgc tgcgtggttc
     4921 ggctcgtgac cgaggtgctt gaccgactgg tccacgcacg gtcaggagac ggtgccgttg
     4981 tcgtgaccgc ggcatacgcc aaggtcacgt cgctctggat ggtgcgggag cgcaactgga
     5041 ggacgccact gtcagtgact gccgtgtcct gcgcttcgag gtgctggctg cgcgcccact
     5101 tctcccatcg accctgcacc gtgaaggcgc ggtcggatgg atagacgggg agggcttcga
     5161 ggttgcgccg gtcaccgtcg agtcctgtgc cgatctcgac ggtggcgtcg tgtccgagcg
     5221 gagtgacctc gacaacgagg gcgagggtgt cgcgagcgac ctgactggcg aatcgcactg
     5281 tgtccaggcg cgattggcgc ccgtgcggat cctcaaagac cgtcgatcgc cacagcactc
     5341 cctgcctcag gtcgagggcg cgctcgtggg agaccacccg gcacgagtgg acgtcgaggc
     5401 gcaccccctc cacgtgcacc tcgacgccga cccagtcagg ggcgttgacc atgtcggtca
     5461 cgatggcgtc gtgggcgtcg aagaccccgt tgacatagac gccgggcaga gccccagcgt
     5521 gtccttcttc caggctgcct cgggtcccga tgcgtccgtt gcccgtcgtg aagagtgtct
     5581 cccaggcgtt ggcccgggcg aggtcacaac cgttctcgcg caacagccag tcctcactgc
     5641 cgaggatcgt gtcgagcatg ctcgtcaccc gccgaccggt gccgtggtgg cgccgccata
     5701 ggtctgtgcg agctgggcat cggtctgtgc gagggggaga tagccgtcga gttcggaggc
     5761 cgaggcacgc tgggcgccca tggcattgcc gtactgacag gcgcggacga tgtcgccgtc
     5821 ctggaggtag ccgaacgcca tgcccgcagc gaaggagtcg ccgcacccgg tcgtgtcgac
     5881 gacgtcctcg atggggatgc gggggacgac gtggctctgg agggctccgg cgtcgtcgag
     5941 ggtgaaggcc acgcagccct cctcgtcgag cgtgacgcag acagccttca cgccttgacc
     6001 caacacgtga gcagcgaagt ccggcagctg atccacggcg atgggatcac cgggtctgcg
     6061 gtcatcgccg gggccgggca tccagctgca ccctgcctcc tcgaggttca tcttgaggat
     6121 gtcgatcgac ggcaaccatg cgtcacgatc ggcccagagt cgatgctctc gggctccgtc
     6181 atggaccaag gtgctcgtgg gtccgtgacc gtcgagcagg atgacgccct cgctgttggc
     6241 cttgatgtat tcgagggtcg actggctcac ctcgtagtca gtgatcggca cgcagacgaa
     6301 ggcgtcggcg tgcaacgcga actcgacgtc gacggggcgg atcggtgcca tgaagccggt
     6361 ctgtcgctcg atgcgcgtgt tctggtcgac gtagcgcagc tcgacgacat caccgcgatc
     6421 cgtggtcgag cggatcccgg tgacgtcgac gttggggtag ctcgcgaaca ggtccttgat
     6481 cggctgctcg tcgaccttct cgacatggac gatcgggagc acccggtcct cctcaccgag
     6541 cagcgccgac agggcgatgg cggtgtagag agcgcagccg tacttctcga ggacgtcacc
     6601 gcggtgggtg atgatgcggt cgcgggggat cggcccgagg acggcgacag tgcgtgcgga
     6661 catgaggtac ctccagaagg ggagcgaacg gcatacgcca aaagtggtga gggtgacatg
     6721 gggcgcgcga ggggaccggc gcgccccacg tcgaagtcga ggtcaggtct tgggtgaccc
     6781 ggtctcgagg aaggcgtcca tgtcctcgag ggactgaccc ttggtctcac ggacccactt
     6841 gctgacgaag atgaatgaca ggacggcgca cagggctttg aagccgtagg ggacaccgag
     6901 cgagaagtcc ttcagcgagg ggaagctcac cgtgatgagc c
//
LOCUS       DAOMZY010000183         6911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_10282_length_6911_cov_4.408985, whole genome shotgun sequence.
ACCESSION   DAOMZY010000183 DAOMZY010000000
VERSION     DAOMZY010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6911
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10282_length_6911_cov_4.408985"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="PLK64_10675"
     CDS             complement(<1..1371)
                     /locus_tag="PLK64_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HPV80255.1"
                     /translation="MQALIPAAPEVALEGLNLTLVLVVLVISLIALAMGAMFRRQVLA
                     ADPGTERMQEIGAAVQEGAQAYLQRQFKTLGGFVVLVFLLLFLLPAADMGVKVGRSVF
                     FVIGALFSASIGYLGMNLAVRANVRVASAARQGDREGGMRIAFRTGGTVGMATVGLGL
                     LGASIVVLLYKGEAPKVLEGFGFGAALLAMFMRVGGGIFTKAADVGADLVGKVEAGIP
                     EDDPRNAATIADNVGDNVGDCAGMAADLFESYAVTLVAALILGSVALGVTGLVFPLIV
                     PAIGAITAIIGVFITKVKGNENGLTAINRGFYISAVISAVLSLIAAFVYLPQSTDAAI
                     LGVQIDGLRIRATMAVFLGIILAGIILWLTGYFTGTTSRPTNDVARTSLTGPATVVLS
                     GIGVGFESAVYTAVIIGAAVYGAFTLGAGSTLLALFFIALAGCGLLTTVGVIVAMDTF
                     GPVSDNA"
     gene            complement(1621..1962)
                     /locus_tag="PLK64_10680"
     CDS             complement(1621..1962)
                     /locus_tag="PLK64_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPV80256.1"
                     /translation="MDLALSTTAQGERTIVHVGGEIDVYTAPQLRRRLDEPIHAGCKD
                     LVVDLGRVTFLDSTGLGVLVGRLKMVRSQGGRMRIVGATDRVLKVFTITGLDRVLEMF
                     PTLADVPDDLA"
     gene            2026..4467
                     /locus_tag="PLK64_10685"
     CDS             2026..4467
                     /locus_tag="PLK64_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPV80257.1"
                     /translation="MPTPHETRPDPAEAARADPAALLSRLASGDRQARLRHTRRIPER
                     AARTADLPSWVTPSLAGALLGEGIATLWSHQVETAEAAHGGEHVVVATGTASGKSLGY
                     LVPVLSDVLDGASAPTGRGATALYLAPTKALAHDQLDRVTRLALPGLRVATYDGDTAP
                     EERRWIREHGAYVLTNPDLVHHSLLPGHERWAPFLRALRYVVIDECHVYRGVFGSHLA
                     LVLRRLRRVAARYRSYPTFVLASATVADPGGHAARLIGMPVRPVTLDGSPRAAVTIGL
                     WEPSIRPVTDAESGVTARRRSAVAEAADLLADLVDEGVQTVAFARSRVGAEVLAQAAT
                     RRSADRGGAAADHYEAGGSRGETTADRDGTVGSGAVIAAYRGGYLPEERRILEGQLRS
                     GHLRGLAATTALELGIDVSGLDAVLLAGWPGTVSSFWQQVGRAGRAGRESLAVLVAAD
                     DPLDGFLVRNPEAIFDRPVEVAVVDPDNPHVLAPHLVCAAAELPVTDADLAPDLFGPG
                     AGPLLDRLVADGVVRRRPTGVYWARDEPPAASVSLRGIGQTVRVVERSTGRVVGLVDE
                     SRADAAVHPGAVHVHQGRSFVVTALDLDEGSALVVAGDPGWSTQARSVSEFNLGKVRE
                     QVSSGPLTAAFGEVTVHHQVMSFLRRLPDGEVIGEHPLDLPVRTLSTTGVWWTLPEEA
                     LLDVGVDASGIPGALHAAEHAAIGMLPLVASADRWDIGGVSTARHPDTGLPTVLVYDG
                     YPGGAGFAQRGYAALRDWLAATRDVVAGCACRDGCPSCVQSPKCGNGNHPLDKAGAVR
                     VLAHVVSLLERCGTE"
     gene            4536..4835
                     /locus_tag="PLK64_10690"
     CDS             4536..4835
                     /locus_tag="PLK64_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80258.1"
                     /translation="MVAFGLILLLIGVGAGGWLAWLALQSETGVTLGATGLQLTVLPI
                     TLFVAGAASMLLLWLGVRFMALGTKRRVARRKELNELRTAPPAPGGAGPRSTDGL"
     gene            complement(4849..5193)
                     /locus_tag="PLK64_10695"
     CDS             complement(4849..5193)
                     /locus_tag="PLK64_10695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80259.1"
                     /translation="MSRERGQSTVAVLGVLGALVASTVGALDLARAVNAAHVARAAAD
                     LAALAGAGAGVRGAGAGEACAQASRLAALNHAQLVACAVPGEGSVEVSVTATSGGPWP
                     RTARASARAGPG"
     gene            complement(5190..5516)
                     /locus_tag="PLK64_10700"
     CDS             complement(5190..5516)
                     /locus_tag="PLK64_10700"
                     /inference="COORDINATES: protein motif:HMM:NF041390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family type IV pilus minor pilin"
                     /protein_id="HPV80260.1"
                     /translation="MATAEFAVALPAVVLVLVMALSAIGAGIDQIRCVDAARLAARAL
                     ARGDSQGTAAALARGAAPSGASVTLSAGGDLATAMVSVSRRAPVLGFSWVVAASATAP
                     REAAPP"
     gene            complement(5560..5763)
                     /locus_tag="PLK64_10705"
     CDS             complement(5560..5763)
                     /locus_tag="PLK64_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
                     /protein_id="HPV80261.1"
                     /translation="MGKALRWRLIGGWREARRRMDAGMTTAEYAVGTLAAVAFAAALI
                     AVVKSDAVRSALARIIQSALSTG"
     gene            5999..>6911
                     /locus_tag="PLK64_10710"
     CDS             5999..>6911
                     /locus_tag="PLK64_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPV80262.1"
                     /translation="MSAAARLRRLDPSARIVVVEQGSEVSFANCGLPYFLSGEIANRS
                     DLLLRTPESLAATLSLEVRVDTLATAIDPAAREVTVRGPEGQDERIGYDALVLAPGAY
                     AVRPPIPGIDHPRVRTLRTVPDADTIRGWVQQGAARSVVLGAGFIGLEAAEALRHAGL
                     DVSIVEAAPHVLPVIDAELAAYVTDELRTHGLDVHEGVAARAILDVEGTPVVELADGT
                     VLPADLVILSVGVRPRSELAAAAGLALTEQGAIIVDDDQRTSDPHIWAVGDATASTNA
                     ITGAVAPVALATPANRGGRVAADSIAGG"
BASE COUNT         1068 a         2463 c         2418 g          962 t
ORIGIN      
        1 ggcgttgtcg gagacggggc cgaaggtgtc catcgccacg atgacgccga cggtcgtcaa
       61 cagcccacac ccagcgaggg cgatgaagaa gagcgccaac aatgtcgacc cagcgcccag
      121 cgtgaaggcg ccgtagacgg cggccccgat gatgaccgcg gtgtagacgg ccgactcgaa
      181 gcccacaccg atgcccgaga gaacgacggt ggccgggccg gtgagggacg tgcgagccac
      241 gtcgttggtc ggccgggagg tcgtgcccgt gaagtagccc gtcagccaga ggatgatgcc
      301 cgcgagaatg atgccgagga agacggccat ggtcgcgcgg atccggagcc cgtcgatctg
      361 caccccgaga atggcggcgt cggtcgactg cggcaggtag acgaatgcgg cgataagcga
      421 caacaccgcg gagatcaccg cagagatgta gaaaccgcgg ttgatggccg tcaggccgtt
      481 ctcgttgccc ttcaccttgg tgatgaagac gccgatgatg gcggtaatgg caccgatcgc
      541 cgggacgatg agcgggaaca ccaggccggt gacgccgagc gccacggaac cgaggatgag
      601 cgctgcgacc agggtcacgg catacgactc gaagaggtcg gccgccatgc cggcgcagtc
      661 gcccacgttg tcaccgacgt tgtcggcgat ggtggccgcg ttgcgcgggt cgtcctcggg
      721 aatgcccgcc tcgaccttgc cgacgaggtc ggcacccaca tcggcggcct tggtgaagat
      781 gccgccgccg acacgcatga acatcgcgag cagcgccgcg ccgaagccga agccctcgag
      841 gaccttcgga gcctcaccct tgtagagcag gacgacgatc gaggcgccga gcagacccag
      901 gccgacggtc gccatgccga cggtgccgcc ggtgcggaag gcgatccgca tgccgccttc
      961 acggtcaccc tggcgagcgg ccgaggccac ccggacgttg gcgcgaactg cgaggttcat
     1021 accgaggtag ccgatgctcg ccgagaagag agcgccgatg acgaagaaga ccgaccgtcc
     1081 caccttgacg cccatgtccg cggcggggag gaggaagagc aacaggaaga ccaggacgac
     1141 gaaaccgccg agcgtcttga actggcgctg caggtaggcc tgcgcccctt cctggaccgc
     1201 agcgccgatc tcctgcatcc gttcggtacc cgggtcggcg gcgagaacct gacgccggaa
     1261 catcgcaccc atcgccagcg cgatgaggga gatgacgagg acgacgagga caagagtgag
     1321 gttgagccct tcgagggcga cctcgggcgc ggcaggtatc agggcttgca tgcatttcct
     1381 cctagacgcc gcccatggcg gcgcacgggg tcggcgccga acggggggtt cagcggaggc
     1441 acatccgtgt gttccggggc gaggctatcg cccgcaaccg tcgcgaggcg acagtgctgg
     1501 cccgctcagg aggtgtgtaa cggctcacgc cgccgatgaa aacgcttgca gcccaagcca
     1561 atgggaacgt gggtcacgac cgggggcact gccggttggc acgggcgggg tcgaggccga
     1621 tcaggccagg tcgtcgggga cgtccgccag ggtcgggaac atctccagga cccggtcgag
     1681 ccccgtgatc gtgaacacct tgaggacccg gtcggttgca ccgacgatcc gcatccggcc
     1741 accttgggag cgcaccatct tcagcctccc gaccaggacg cccagacccg tggaatccag
     1801 aaaagtgacc cggccgaggt caacgaccag gtctttgcac ccggcgtgga tcggctcatc
     1861 gagtcgccgt cgcaattgag gtgccgtata gacgtcgatc tccccaccga cgtgcacaat
     1921 cgtgcgctcc ccctgcgccg tggtcgacaa cgccagatcc acgatcacaa ccttccgctc
     1981 actgcctttc tttgaacgtt acctcggcac cctacggtga gcacaatgcc cactccgcac
     2041 gagacgcgac ctgacccggc cgaggcggcc cgcgccgacc ccgcggcgtt gctgtcgcgc
     2101 ctggcgagcg gcgatcgtca ggcgcggctg cggcataccc ggcggatccc cgagcgagca
     2161 gcccgcacgg ccgacctgcc gtcctgggtg accccgtccc tcgcgggagc gctcctcggc
     2221 gagggcatcg ccacgttatg gagccaccag gtcgagacag ctgaggccgc gcatggcggt
     2281 gagcatgtcg tcgtcgccac ggggacggcc tcgggcaagt ccctgggtta cctcgttccg
     2341 gtgctctcgg acgtgcttga tggagcatcg gcacccacgg gacgcggcgc gactgcgctg
     2401 tatctcgcgc cgaccaaggc tctggcccac gaccaactcg accgggtgac ccggctcgcg
     2461 ttgccggggc tgcgcgtcgc gacctatgac ggcgacacag ctcccgagga gcggcggtgg
     2521 atccgcgagc acggggccta tgtgctgacc aaccccgacc tcgtccacca ctcgttgttg
     2581 cccgggcacg agcggtgggc tccgttcctg cgcgccttgc gctatgtcgt catcgacgaa
     2641 tgccacgtct accgaggggt tttcgggtcg cacctcgcgc tggtcctgcg gcggttgcgc
     2701 cgggtcgcag cccgctaccg ctcctacccc accttcgtcc tcgcctccgc gaccgtcgcc
     2761 gacccgggtg ggcacgcagc gcgcctgatc ggtatgccgg tccgcccagt cacgctcgac
     2821 ggctcgccac gcgcggcggt gaccatcggg ttgtgggagc cgtcgatccg gccggtgacc
     2881 gatgccgaat cgggggtgac cgcgcggcgg cgttcggccg tggccgaggc tgcggatctg
     2941 ttggccgatc tggtggacga gggcgtgcag acggtggcct tcgcccgatc gcgggtcgga
     3001 gcggaggtgc ttgcccaggc agccacccga cgctccgccg atcgaggcgg ggccgccgcc
     3061 gatcactacg aggccggcgg aagccggggc gagaccaccg ccgatcgtga cggaaccgtc
     3121 gggtcgggag cggtgatcgc ggcataccga ggcgggtatc tgcccgaaga acggcggatc
     3181 ctggaggggc agttgcgctc cggtcacctg cgcggcctcg cagcgacgac cgcgctggaa
     3241 ctcggcatcg atgtcagcgg cctggacgcg gtgctcctgg cgggttggcc ggggacggtg
     3301 tcgtcgttct ggcaacaggt gggccgagcc gggcgggctg gccgcgagtc gctggcggtg
     3361 ctcgtcgccg ccgacgaccc gctggacggc ttcctcgtgc gcaacccgga ggcgatcttc
     3421 gaccggcctg tcgaggtcgc ggtcgtcgac ccggacaacc cacatgtctt ggccccccac
     3481 ctcgtctgcg cggcggccga actgccggtg accgacgccg acctcgcgcc ggacctgttc
     3541 gggccgggag ccgggcctct gcttgaccgg ttggtggcag acggtgtcgt gcggcgccga
     3601 cccaccggcg tctactgggc ccgtgacgag ccaccggctg cgtcggtgtc gttgcgcggg
     3661 atcgggcaga ccgtgcgggt cgtggagcgc tcgaccggcc gggtcgtggg cctcgtcgac
     3721 gagtcgcgcg ccgacgcggc ggtccaccca ggcgccgtcc acgtccacca ggggcgcagt
     3781 ttcgtcgtca ccgcgctcga cctcgatgaa ggctcggccc tcgtcgtcgc cggcgacccg
     3841 ggctggagca cgcaggcgag gtcggtgagc gagttcaacc tggggaaggt gcgtgagcag
     3901 gtgtcgagcg gcccgttgac cgccgcgttc ggcgaggtga ccgtgcacca ccaagtgatg
     3961 tccttcctgc gccgactgcc cgacggcgag gtcatcgggg agcacccact cgacctcccg
     4021 gtgcggacgc tctcgacgac cggcgtctgg tggaccctcc ccgaggaggc gctgctcgac
     4081 gtgggcgtgg acgcgagcgg cataccgggg gccctgcacg ccgccgagca cgcggcgatc
     4141 gggatgctgc ccctcgtggc gagcgccgac cgctgggaca ttggtggggt gtcgaccgca
     4201 cggcacccgg acacggggtt gcctacggtg ctcgtctacg acggctaccc gggcggggcc
     4261 gggttcgcgc agcgcgggta tgccgccctg cgcgactggc tcgcggcgac ccgtgacgtc
     4321 gtggcggggt gcgcctgccg ggacggctgc ccgtcgtgcg tccagtcgcc caagtgtggc
     4381 aacggcaacc atccgctgga caaagcgggc gccgtgcggg tgcttgccca cgtggtgagc
     4441 ctgctggaac gttgcggcac tgagtagtgt cgccaccgaa gccgtcgaat cgccggccgc
     4501 ggtcgacggc gtgggtggga catcaggagg acaacgtggt cgcattcggg ttgatcctgc
     4561 tcctcatcgg ggtgggcgcg ggtggctggc tcgcgtggtt ggccttgcag agcgagaccg
     4621 gggtgacgct gggagcgacc gggctgcagt tgaccgtgct gccgatcacg ctgttcgtcg
     4681 ccggtgccgc gtcgatgctg ctgctgtggc tcggggtgcg attcatggcc ctcgggacca
     4741 agcgtcgggt cgcccggcgc aaggaactca acgagttgcg cacagcgccg ccggcgcccg
     4801 gtggtgcggg tccgcgcagc actgacggtc tctaaggtct cgacgggctc agccagggcc
     4861 agcccgcgcg gaggctcggg ccgtcctcgg ccacggcccg cccgaagtcg ccgtgacgga
     4921 gacttcgacc gaaccctcgc ccggcaccgc acacgccacg agctgcgcgt ggttgagcgc
     4981 ggccaaccgg gaggcctgag cgcaggcctc tcccgcgcca gcaccgcgaa cccctgcgcc
     5041 cgcacccgcc agcgcggcga gatcagcggc agcccgcgcg acgtgggcgg cgttgaccgc
     5101 tctggccagg tcgagcgctc cgaccgttga cgccaccagc gcgcccagga ctccgagcac
     5161 agccacggtg ctttgaccac gctcacggct cacggcggag ccgcctcacg gggagcagtc
     5221 gccgacgccg ccacgaccca ggagaagccc agcacgggcg cccgacgcga caccgagacc
     5281 atcgctgtcg ccaggtcgcc accggcgctc agggtcactg atgcacccga gggagccgcg
     5341 ccacgcgcca acgcggccgc ggtgccctgg gagtcgcccc tggccaacgc ccgcgcggcc
     5401 agccgggcag catcgacaca ccggatctgg tcgatcccgg ctccgatagc gctcagcgcc
     5461 atcaccaaca cgagcaccac cgcaggcagc gccaccgcaa actcggccgt cgccatcccg
     5521 cgctcgctgc gaccacgcgg acgcctcacc gggccgccct caccccgtgc tcagcgccga
     5581 ctgaataatg cgagccaagg ccgaacgcac cgcatcagat ttcacgaccg cgatcagcgc
     5641 cgcagcgaac gccaccgcgg ccagcgtgcc cacggcatac tccgctgtcg tcatgccggc
     5701 gtccatgcgg cggcgagcct cacgccagcc gccaatcagc ctccacctca aggcttttcc
     5761 catcatcaca cgtcccttca gtcgcagacc gatacctcga tcctcaccgc cgacccgacc
     5821 tgttgtcagc gcctcccgcc cggcctgtgg atgatgggac aactgaccag gtgggtgtgg
     5881 acaacccgga cccgcgcgcg ctgcataccc ctaggggtat gcttcaaccc gaactgaaga
     5941 gtcaaggagc gtggcagtcg tgagaaccgt cgtggtcatc ggtggagtcg ccggggggat
     6001 gagtgccgcc gcgcggctgc gccgattgga ccccagcgcg cgcatcgtcg tcgtcgagca
     6061 gggctcggag gtgtcgttcg ccaactgcgg gctcccctac ttcctcagcg gcgagatcgc
     6121 caaccgcagc gacctgctcc tgcgcacgcc cgagtcactc gccgccaccc tcagcctcga
     6181 ggtccgcgtc gacaccctcg ccaccgcgat cgacccggcc gcccgcgagg tcaccgtgcg
     6241 cggccccgaa ggccaagacg agcgcatcgg ctacgacgcg ctcgtcctgg cccccggggc
     6301 gtatgccgtc cgcccaccga tccccggcat cgaccacccg cgggtgcgga cccttcggac
     6361 ggtccccgac gcggacacga tccgcggctg ggttcagcag ggcgccgcac gctccgtcgt
     6421 cctgggcgcc gggttcatcg gcctggaggc cgccgaggcc ctgcgccacg ccggcctgga
     6481 cgtgagcatc gtcgaagcgg ccccccacgt tctcccagtc atcgatgccg agctcgcggc
     6541 atacgtgacc gatgaactgc gcacccacgg gctcgacgtg cacgagggag tcgccgctcg
     6601 cgcgatcctc gacgtcgagg gcaccccggt cgtcgagctc gccgacggca cggtcctgcc
     6661 cgccgacctg gtcatcctgt cggtcggggt caggccgcgc tcggagctcg ccgcggccgc
     6721 cggcctggcc ctcaccgagc agggggcgat cattgtcgat gacgaccagc gcacctccga
     6781 cccgcacatc tgggccgtcg gcgacgccac ggccagcacg aacgcgatca ctggggccgt
     6841 ggctcctgtc gccctagcca cccccgccaa ccggggtggc cgagtggccg ccgactcgat
     6901 tgcggggggt g
//
LOCUS       DAOMZY010000184         6875 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_10338_length_6875_cov_5.936804, whole genome shotgun sequence.
ACCESSION   DAOMZY010000184 DAOMZY010000000
VERSION     DAOMZY010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6875
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10338_length_6875_cov_5.936804"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PLK64_10715"
     CDS             complement(<1..561)
                     /locus_tag="PLK64_10715"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPV80263.1"
                     /translation="MTSFESINVGDQGHVAHQYATTERLRGRMSVWGPGATGLDPTDV
                     LFEELVARSPRTVLEIGCGTGAFAKRVIDALPDVDYLATDASPAMVAAASAQGVRAEV
                     RPAEATGQPTGSIDVVVAAWMLYHVPSLDAALAEVCRVLRPGGALLAVTNGEQHLATL
                     FAAAGVTPPRSTFTSENGEALLERHFD"
     gene            complement(781..3558)
                     /gene="aceE"
                     /locus_tag="PLK64_10720"
     CDS             complement(781..3558)
                     /gene="aceE"
                     /locus_tag="PLK64_10720"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HPV80264.1"
                     /translation="MTGRGTTGPILNGIPSQLPDTDPEETQEWLDSLQGVIDEQGRTR
                     ARYLMLQLLAAARGQQVGVPSLTATDYINTIPPEREPWFPGDEDIERAYRRWIRWNAA
                     VMVHRAQRPGVGVGGHISTYASAATLYEVGMNHFFRGQDHPGGGDQVFFQGHAAPGMY
                     SRAFLEGVLEEEDLDGFRQERSHVVDGKIRALSSYPHPRLMPNFWQFPTVSMGIGPMN
                     AIYQAQFNRYLLNRGIKDTSDQHVWAFLGDGEMDEPESRGLLQLAANEELDNLTFVVN
                     CNLQRLDGPVRGNGKIIQELEAFFRGAGWNVIKVVWGRGWDPLLAADRDGALANLMNQ
                     TSDGDYQTFRANDGKYVREHFFWRDPRTRKMVENWTDDEVWFKLRRGGHDYRKVYAAY
                     RAALEHTGQPTVILVKTIKGFTLGSHFAGRNATHQMKKLTLEDLKGFRDLLRIPITDE
                     QLEADPYRPPYYRPDASDPAMQYMRERRRVLGGSVPSRRVEHAPLHLPGDDAYAAVTK
                     GSGKQAVATTMAFVRLLKDLMRDKEFGQRIVPIIPDEARTFGMDSFFPTIKIYNPHGQ
                     NYTPVDHDLMLAYRESEQGQILHLGINEAGSLAAFTAAGTAYATHGQPMIPIYVFYSM
                     FGFQRTGDSIWAAADQMTRGFLIGATAGRTTLTGEGLQHADGHSPLLASTNPAVVAYD
                     PAYGYEIAHIMKDGLRRMYGEPEDPRGRNAIYYITVYNEPYVQPAQPADLDVEGVIRG
                     IYKLHAADTSAWTHTAPRAQLLASGVGVPWALEAQKLLADDFGVAADVWSVTSWSELR
                     RDAMACDEHAFLHPEEPAKVPFVTQALTGAPGPVLAVSDYMKAVQDQIGPWVPGGLTS
                     LGADGFGFSDTRQAARRAFHIDGPSLAVRTLQVLAARGEAPIEWARTAAEKYRLLDIN
                     AGTSGSAGGDA"
     gene            3773..4201
                     /locus_tag="PLK64_10725"
     CDS             3773..4201
                     /locus_tag="PLK64_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HPV80265.1"
                     /translation="MHGSAGVAAFTSGLSKLGFTPGQVVQELGWDDDVDEALRAGVEQ
                     VVGSALEDEGYADGADGVLVWFRSFDGDLVDVLVDALTNLVDRGCLVLATPRADQDGH
                     VEPSEIEDAAVAAGLHPSGVANLSREWTVQRLVAPRSQRR"
     gene            4250..4759
                     /locus_tag="PLK64_10730"
     CDS             4250..4759
                     /locus_tag="PLK64_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPV80266.1"
                     /translation="MSAEPDAVAPAAPEVGDVPPDFTLPDQYGRQVSAATWRGDRNVV
                     LVFYPFAFTGICTGELREIRDTLEDFQHDGIQVLAISCDPVPALRAWADQEGYFFPLL
                     SDFWPHGEVSRAFGVFDTDGGFATRGTFLLDRDGVVRWRMLNGPGDARDFGSYREALA
                     GLRADAARA"
     gene            4802..4874
                     /locus_tag="PLK64_10735"
     tRNA            4802..4874
                     /locus_tag="PLK64_10735"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4835..4837,aa:Val,seq:tac)
     gene            4882..5718
                     /locus_tag="PLK64_10740"
     CDS             4882..5718
                     /locus_tag="PLK64_10740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HPV80267.1"
                     /translation="MTLGQIVALLDGFYPPATALSWDRVGLVTGDPDQPIGHVRFAVD
                     PTLAVIEEAIADGADLLVTHHPLLLRGIHSVATTTAKGRAVTRAVVADLAILCAHTNA
                     DVADPGVCDALAAACGLVDTEPLDLEQGQGVGRVGRLAQPTTLRAFAEGLAAALPPTN
                     VGVRVSGDPDAQVCRVAVLGGSGDDRFGAARRSGVDVYVTADLRHHPALEAREESSFG
                     PPYLIDAGHYATEWLWLSGAAARLSQALAEQGTTVETSISTLRTDPWDFLVGVNADGG
                     TP"
     gene            5715..6455
                     /locus_tag="PLK64_10745"
     CDS             5715..6455
                     /locus_tag="PLK64_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HPV80268.1"
                     /translation="MKAQPHQQARLIEVQLLDTRLDQLAHARAHLPALTKLAGLDQAQ
                     VTLDRELALARTALSDVQREVDKAEADVQLVRARAARDQARLDSGTGTPKDLQALQSE
                     LVSLARRQGDLEEIELEVMERAESLEADVASLETRRSALLEELAQVRAERDAAFEVSD
                     AEAADVTERRSALVPEVGAELLALYDKIRASVGGTGASELRQRRCGGCRLELNQVDLS
                     RIKAAPADEVIRCEECRRILVRTAESGL"
     gene            6458..>6875
                     /locus_tag="PLK64_10750"
     CDS             6458..>6875
                     /locus_tag="PLK64_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HPV80269.1"
                     /translation="MARRFIVEADGGSRGNPGVAGFGALVRDADTGAVLLERAEPLGL
                     ASNNVAEYAGLLAGLEGALRLDPAPDIEVRMDSKLVVEQMSGRWKIKHEDMRRLAGQC
                     QDVVRRILAGGGRVRYTWIPREKNKAADALSNHAMDG"
BASE COUNT         1036 a         2334 c         2406 g         1099 t
ORIGIN      
        1 gtcgaagtgc cgctccaaca aggcctcacc gttctcggag gtgaacgtcg agcgaggtgg
       61 ggtcaccccg gctgcggcga agagcgtcgc gaggtgctgc tccccgttgg tgacggcgag
      121 cagggcacct ccggggcgca gcactcggca cacctcggct agggcggcgt caagcgatgg
      181 cacgtgatag agcatccagg ccgcgacaac cacatcgatc gagccagtcg gttgtccggt
      241 ggcctcggct ggccgcacct cagcccttac accctgggcg cttgcggcgg ccaccatcgc
      301 gggtgacgca tcggtggcga ggtagtcgac gtctggcagc gcgtcgatga cccgcttggc
      361 gaaagctccc gtgccgcaac cgatttccaa gactgtccga ggcgaccttg ccaccaactc
      421 ctcgaacagg acgtccgtcg gatccaggcc agttgcgcca ggcccccaca cggacatccg
      481 gccgcgaagc cgctccgtgg tggcgtactg gtgggcgaca tgtccctggt caccgacgtt
      541 gattgactcg aaggaggtca ccccggcagt atcccgccag cacagcgaat accaatggtt
      601 gggctctccg cggctggcca cgctcaacag ggcgcccagc tcagaccatt ggccttcggt
      661 gtgcgcagca aggcccggga agccgcgcgg cgcggagcgg gtgggaggga agccgccgcg
      721 gcaaaggaga ggcgggcggc atacggtatg ccgcccgcct tcctctagcg aaaaccagac
      781 ctacgcgtcg ccgcccgcac tgccggaggt gccggcgttg atgtcgagca ggcggtactt
      841 ctcggctgcg gtgcgagccc actcgatggg ggcttcgcca cgcgcggcga gcacctgcag
      901 ggtgcgcacc gccagtgacg gaccgtcgat gtggaaggcc cgccgcgccg cctggcgagt
      961 gtcggagaag ccgaacccgt cggcgccgag cgaggtgagt ccaccaggca cccacggacc
     1021 gatctggtcc tggacggcct tcatgtagtc cgagacggcg agcacggggc ccggcgcgcc
     1081 ggtcagggcc tgggtgacga acggcacctt ggccggctcc tcggggtgca ggaaggcgtg
     1141 ctcatcgcac gccatcgcgt cccggcgcag ctcgctccac gacgtgaccg accagacgtc
     1201 agcagccact ccgaagtcgt cggcgagcag cttctgggcc tccaacgccc acggcacgcc
     1261 aacgccagag gcgagcaact gggcccgagg cgcagtgtgc gtccaggccg acgtgtcggc
     1321 ggcatgcagc ttgtagatac cccggatgac gccctcgaca tcgaggtcgg caggctgggc
     1381 cggctgcacg tagggctcgt tgtagaccgt gatgtagtag atcgcgttgc gaccgcgcgg
     1441 gtcctcgggc tcgccgtaca tccgacgcag cccgtccttc atgatgtgcg cgatctcgta
     1501 gccgtaggcc gggtcgtagg ccacgaccgc cgggttggtc gaggccagca gcggcgagtg
     1561 accgtcggcg tgctgcaacc cctcgccggt gagcgtcgtg cggcccgccg ttgcgccgat
     1621 gaggaacccg cgcgtcatct ggtcggcggc ggcccagatc gagtcgccgg tgcgctggaa
     1681 cccgaacatc gagtagaaga cgtagatcgg gatcatcggc tggccatgcg tggcgtatgc
     1741 cgtgcccgct gcggtgaacg ccgcaaggga cccggcctcg ttgatgccca ggtgcaggat
     1801 ctgaccctgc tcggactcgc ggtaggccaa catcaggtcg tggtcgaccg gcgtgtagtt
     1861 ctggccgtgg gggttgtaga tcttgatcgt cgggaagaac gagtccatcc cgaaggtccg
     1921 cgcctcgtcc gggatgatcg gcacgatgcg ctggccgaac tccttgtccc gcatgaggtc
     1981 cttgagcagc cgcacgaacg ccatagtcgt cgcgacggcc tgcttgcctg atcccttggt
     2041 gacggcggca taggcgtcgt caccgggcag gtgcaggggg gcgtgctcga cccgacgcga
     2101 cgggaccgac ccgccgagga cgcgccggcg ctcgcgcatg tactgcatcg ccgggtcgct
     2161 ggcgtccgga cggtagtacg gcgggcggta ggggtcggcc tcaagctgct cgtccgtgat
     2221 cgggatgcgc agcaggtcac ggaagccctt gaggtcttcc agggtgagct tcttcatctg
     2281 gtgcgtggcg ttgcggccag cgaagtgcga cccgagggtg aagcccttga tggtcttgac
     2341 caggatgacg gtcggctggc cggtgtgctc cagagcggcg cggtaggcgg catacacctt
     2401 gcggtagtcg tgcccgccgc ggcgcagctt gaaccacacc tcgtcgtcgg tccagttctc
     2461 gaccatcttg cgggtgcgcg ggtcacgcca gaagaagtgc tcgcggacgt acttgccgtc
     2521 gttggcccgg aaggtctggt agtcgccgtc agaggtctgg ttcatgaggt tggccagtgc
     2581 cccgtcgcgg tcggcggcga gcagcgggtc ccacccgcgg ccccagacga ccttgatgac
     2641 gttccaaccg gcgccgcgga agaacgcctc gagctcttgg atgatcttgc cgttgccgcg
     2701 gaccgggccg tcgaggcgct gcaggttgca gttgacgacg aaggtgaggt tgtcgagctc
     2761 ctcgttggcg gccagctgca gcagcccacg cgactcgggc tcgtccatct caccgtcgcc
     2821 gaggaacgcc cacacgtgct ggtcgctcgt gtccttgatc ccgcggttga gcaggtagcg
     2881 gttgaactgc gcctggtaga tcgcgttcat cgggccgatg cccatcgaca ccgtcgggaa
     2941 ctgccagaag ttcggcatga gccgcgggtg cgggtaggac gacagcgccc ggatcttgcc
     3001 gtcgacgacg tgggagcgct cctgacggaa accgtcgagg tcttcctctt caagcacgcc
     3061 ctcgaggaag gcccgcgagt acatccccgg agccgcatgc ccctggaaga agacctggtc
     3121 accgccaccc gggtggtcct ggccgcggaa gaagtggttc atcccgactt cgtagagcgt
     3181 cgcagcggac gcataggtcg agatgtgccc gccgacgccc acgcccgggc gctgagcccg
     3241 gtggaccatc acggccgcgt tccagcggat ccaccggcgg taggcccgct cgatgtcctc
     3301 gtcaccgggg aaccagggct cacgctccgg cgggatggtg ttgatgtagt cggtcgcggt
     3361 cagcgacggc accccgacct gttgaccacg cgcggcagcc agcaactgca acatgagata
     3421 gcgcgcccgg gtccggccct gctcatcgat cactccttgg agcgagtcga gccattcctg
     3481 cgtctcctcc ggatcggtgt ccgggagttg gctcggaatg ccgttgagga tcggcccggt
     3541 ggtgccacga cctgtcacgg gggacttcct ttctgagcaa catggatgcg cccatcctgt
     3601 accccgatgc gcgctgcgtc acattcgtcc cgccagtcga tccgacgcgg acccgatttc
     3661 ggtgacgatg cggcatacgt gagcgtcaac acagcccgtc ggcgtcccgc ctcgggcaga
     3721 ctgggaccga gcgagcggct agtggagggt cgccacggca ggaggggtcg gcgtgcacgg
     3781 gtcggcgggt gtcgccgcat ttacatcggg tctcagcaaa ctcgggttca cccccgggca
     3841 ggtcgtccag gagttgggtt gggacgacga cgtcgacgag gcgctgcgcg ccggtgtcga
     3901 acaggtcgtc ggctcggccc ttgaggacga gggctacgcc gacggcgccg acggggtgct
     3961 cgtctggttt cgatccttcg acggtgatct cgtcgatgtc ctcgtcgacg cgctgaccaa
     4021 cctcgtggac cgcggctgcc tcgtcctggc cacgccccga gccgatcagg acgggcacgt
     4081 cgagcccagc gagatcgaag acgcggccgt ggctgccgga ctgcacccat cgggtgtcgc
     4141 caacctcagc cgggagtgga ccgtgcagcg actggtggcc cctcgatcgc agcgtcgctg
     4201 accctcacgt cgaccggtcg ggctcgcgcg ggtggcagaa ttgatcgtca tgagcgctga
     4261 acccgacgcc gtggcgcctg ccgcgcccga ggtcggggac gttcccccgg acttcaccct
     4321 tcccgaccag tacggccgcc aggtcagcgc ggccacctgg cgcggggacc gcaacgtcgt
     4381 cctcgtcttc tacccgttcg ccttcacggg catctgcacc ggggagttgc gcgagatccg
     4441 cgacaccctc gaagacttcc agcacgacgg catacaggtg ctcgcgatct cctgcgaccc
     4501 ggtgccggcg ttgcgcgctt gggccgacca ggaggggtac ttcttccccc tcctgtcgga
     4561 cttctggcca cacggcgagg tctcccgggc cttcggggtc ttcgacaccg acggcggctt
     4621 cgcgacgcgc ggcaccttcc tgctcgaccg ggacggtgtg gtccgttggc ggatgctcaa
     4681 cgggccgggc gacgctcgcg actttggtag ctaccgggaa gccctcgcag gcctgcgcgc
     4741 agacgccgcg cgcgcctaac tgacacaatg ggcgccgctc gcccccgtgg gcggcgagtt
     4801 ggggcctgta gctcagttgg tagagcaccg cgcttacacc gcgggtgtcg tcggttcgag
     4861 cccggccggg cccacatgac ggtgacgctt ggacagatcg tcgcgctgct cgacggcttc
     4921 tatccgccgg cgacggcgtt gtcgtgggac cgggtcggcc tggtgacggg ggaccccgac
     4981 cagccgatcg ggcacgtgcg gttcgccgtc gaccccacct tggcggtcat cgaggaggcc
     5041 atcgccgacg gggcggacct attggtcacc caccacccgc tgctgctgcg cggcatacat
     5101 tccgtcgcga cgaccacagc gaagggccgc gcggtgaccc gagccgtggt cgccgacctc
     5161 gcgatcctct gcgcccacac caacgccgac gtcgcggacc cgggcgtgtg cgacgcgctc
     5221 gccgccgcct gcgggctggt ggacaccgag ccgctcgacc tcgagcaggg ccaaggggtc
     5281 ggtcgggtgg gtcgcctcgc gcagcccacg acgttgcgtg ccttcgctga agggctcgct
     5341 gccgcccttc cgccgaccaa cgtcggcgtc cgcgtgtctg gagaccccga cgcccaggta
     5401 tgccgtgttg ccgtcctcgg cggttcgggc gacgaccgct tcggcgcggc ccgacgcagc
     5461 ggggtcgacg tctacgtcac cgccgatctg cgtcaccacc cggcgctgga ggctcgcgag
     5521 gaatcgtcct tcggcccgcc gtatctcatc gacgcgggtc actacgccac cgagtggctc
     5581 tggctgtccg gggccgccgc ccgcctgtcg caagcgctgg ctgagcaggg cactacggtg
     5641 gagacgtcga tctcgacctt gcgcaccgac ccgtgggact tcctcgtcgg tgtgaacgcc
     5701 gatggaggta ccccctgaaa gcgcaaccgc accagcaggc ccggctgatc gaggtccaac
     5761 tgctcgacac ccggctcgac caattggccc atgcccgcgc tcacctgccg gccttgacca
     5821 aactggccgg cctggaccag gcccaggtca ccctcgatcg cgagctcgcg ctggcccgga
     5881 cggcgctgtc ggacgtgcag cgcgaggtcg acaaggccga ggccgatgtc caactcgtgc
     5941 gtgcgcgcgc cgcccgtgat caggcccgcc tcgactcagg caccgggacc cccaaggacc
     6001 tgcaggccct gcagagcgaa ctcgtttcgc tggcccgccg ccaaggcgac ctcgaggaga
     6061 tcgagttgga ggtcatggag cgggccgagt cgctcgaggc cgacgtcgct tcgctcgaga
     6121 cccgtcgcag cgcgttgctg gaggagttgg cacaggtgcg cgccgaacgc gacgccgcct
     6181 tcgaggtgtc cgacgccgag gccgctgacg tcaccgaacg ccggtcagcc ctggtgccgg
     6241 aggtgggcgc ggagttgctc gccctctacg acaagattcg tgcgagtgtc ggcgggaccg
     6301 gtgcctccga gttgcgccag cgccgttgcg gcggctgccg tttggagctc aaccaggtcg
     6361 acctgtcccg catcaaggcc gcgccggccg acgaggtcat ccgctgcgag gaatgccgtc
     6421 gcatcctcgt gcgcaccgcc gagtccggac tgtaggcgtg gctagacggt tcatcgtcga
     6481 ggcggacggc ggctcacgcg gcaaccccgg ggtggccggt ttcggcgctt tggtgcgcga
     6541 cgcggacacc ggagcggtcc ttctggagcg cgccgaaccc ctggggttgg ccagcaacaa
     6601 cgtcgctgag tatgccggtc tgctcgccgg tctcgaaggt gcccttcgcc tcgacccggc
     6661 gcccgatatc gaggtcagga tggactcgaa gctcgtcgtt gagcagatgt cggggcgttg
     6721 gaagatcaag cacgaggaca tgcgccgcct cgccgggcag tgtcaggacg tcgtgcgtcg
     6781 gatcctcgcc ggaggcggcc gggttcgcta tacgtggatc ccgcgggaga agaacaaggc
     6841 agccgacgcg ctgtccaacc acgcgatgga cggca
//
LOCUS       DAOMZY010000185         6867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_10350_length_6867_cov_6.189665, whole genome shotgun sequence.
ACCESSION   DAOMZY010000185 DAOMZY010000000
VERSION     DAOMZY010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6867
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10350_length_6867_cov_6.189665"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PLK64_10755"
     CDS             complement(<1..608)
                     /locus_tag="PLK64_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPV80270.1"
                     /translation="MASSTSTVDTVVSLCKRRGFVFPCGEIYGGTRSAWDYGPLGVEL
                     KENIKRQWWKAMVHAREDVVGLDSSIILPRQVWVASGHVGTFTDPLVECLSCHKRERA
                     DHLQEAYAAKKGGGMNPDDVPMSDVPCPHCGNRGQWTEARDFNMMLKTYLGVIEDESG
                     LHYLRPETAQGIFINFKNVEGAARKKPPFGIAQVGKSFRNEIT"
     gene            742..1029
                     /locus_tag="PLK64_10760"
     CDS             742..1029
                     /locus_tag="PLK64_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80271.1"
                     /translation="MSLSFRSKVEHASVQWVTRLNRVPRWAAFIGVLALMVAGILVPK
                     VGFLFTLVIAAFLGWLIFLTWPRLAAPEKLMRIAVLALVVAVAVMQAFPKA"
     gene            complement(1048..1947)
                     /locus_tag="PLK64_10765"
     CDS             complement(1048..1947)
                     /locus_tag="PLK64_10765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV80272.1"
                     /translation="MSALEWRPLTSDDLGQIVALAQECLEADGGLPQLGTDETIQALF
                     LQGEGVTGCDETGEIVAAAAMSRDSAGRVVATGLVHPSMRRQGHGEALVDWCRAHADG
                     QPIRVVAETMSIEAESLFAANNLHRTFAEAVMRHKLKHISRVALPEGLHTEPFRDDTA
                     HAFYAAYRSSFGDRPGFPDPPEEEWLRWLVDDSGFRPEDSRVALTEDGEPAGFITVSD
                     EWIDQVGVVPEWRGQGLGAHLVVRSLTALKKAGASRVWLCVGVDNGARALYERLGFAH
                     KGTRARYEERGAVRRAAREAGLV"
     gene            2088..3053
                     /locus_tag="PLK64_10770"
     CDS             2088..3053
                     /locus_tag="PLK64_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HPV80273.1"
                     /translation="MPRTAPARPRPTSRANRTRVIRAAAWGAVAVLALVSACASPRAA
                     QESAESATAQVPVSAAFYPLAYLLEQVGGPGVRVWTITKPGVEPHDLELTPKDLVDLP
                     KMSLVAYLKGFQPAVDEAVAQQAPSAAYDVSAAADLTLGAPAGEASATDLHFWLDPIR
                     YAAVGTALGERLALADPTHAADYRARAKTFAASMEQLDKTFATGLASCATKQLVTSHA
                     AFGYLAARYGLTQVPIAGLSPDQEPSAKQLADVAATVKAAGVKTIFSETLVDPKFAQT
                     IAQETGATLALLDPVEGITDQSAAKDYPGVMLANLAALRTGLGCS"
     gene            3050..3895
                     /locus_tag="PLK64_10775"
     CDS             3050..3895
                     /locus_tag="PLK64_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HPV80274.1"
                     /translation="MSTPPAPPTPDPPATAPPVIALRGASFGYGDHAVVHDVDLTITA
                     GEVVLVLGPNGSGKSTIAKGLFGLTDLLAGEVDVFGMPRADFRDFPRLGYVPQRHTLA
                     SSVTVTVSEVVSSGRLSRQSWLGIATRADRQQVRRALELVGLADRAGIDVGALSGGQQ
                     RRVLIARALAGEPDVLVMDEPTAGVDIASQHGLVEVLARLVAEGLTLVVVTHELAAFA
                     DLVTRVVVVDAGRIAFDGSRDEFVAAGSDVLHDHDWHHHLDRPDRRPAVISAEGPLSQ
                     GGSRE"
     gene            3892..4914
                     /locus_tag="PLK64_10780"
     CDS             3892..4914
                     /locus_tag="PLK64_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPV80275.1"
                     /translation="MSEMLSIDFMQRALLAALLVGLSAPLVGVFLVQRRLALIGDGMG
                     HVALAGVAVGVWLGQSPVWTALIGAVLAAIIVELIRTSGSAHGDVALAIIFYGGIAAS
                     VVIIGKSPNGTPASLTAYLFGAITTVTAADLWVFGVLTVVILLVVGVLRWRLFAVASD
                     EEYARSAGLPVLALNLALAVLTAVTVVVSMRIVGLLLISALMIVPNAAAQLLGHSFRS
                     SLAWAVAIGVACAVGGVVVSYAADTPSGGTIVLLAIGAFVVAAVGSALRTRLGRVSLA
                     GHAEAEGHGHEHGPGCGHEVIQHGDHVDYVHDGHHHAPHGAHYDEHAEHTDPAEHPEH
                     TQQADR"
     gene            4911..5297
                     /locus_tag="PLK64_10785"
     CDS             4911..5297
                     /locus_tag="PLK64_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPV80276.1"
                     /translation="MTASGRRPTRQQAAVVAYLGETGDFVSAQELHARMRAAGNSIGL
                     ATVYRSLAALATEGALDVLRTDDGEAVYRQCSTDHHHHLVCRTCGRTVEVAGPTVETW
                     AERVAGEHGFRDVRHTVEIVGTCATC"
     gene            5320..6054
                     /locus_tag="PLK64_10790"
     CDS             5320..6054
                     /locus_tag="PLK64_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family zinc transporter"
                     /protein_id="HPV80277.1"
                     /translation="MLEAAWWGLFGGLALVAGSLVGLYGRVSTRVLGLVMAFGAGVLI
                     SAVSFELTLEAFEVGGSSAVVWGLSAGAATFFVGDWLIDQRGGHRRKSPVSQTHVSEG
                     GAIALVLGALLDGIPESAAIGSSLLGGGEVGVAMVAAVFLSNVPESMSASAGLKAAGR
                     SPRYILGLWGLVCLASAVAAALGYAVLGGASPEAVAFVQCFAAGAIITMLGDTMVPEA
                     TEHAGRLVGLFIVAGFTLAFLLSHAA"
     gene            complement(6062..>6867)
                     /locus_tag="PLK64_10795"
     CDS             complement(6062..>6867)
                     /locus_tag="PLK64_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HPV80278.1"
                     /translation="FPHPTGARPPAIPADLIPQHVAIVMDGNGRWANQRGLPRTKGHE
                     AGEAALLDVVAGAIELGIPHLSAYAFSTENWRRSPDEVRFLMGFNRDVIRRRRDQLNS
                     WGVRMRWVGRRPRLWRSVINELEIAQEMTRHNTGLTLYFCVNYGGRAEIADAVVSIAQ
                     EVAAGKLKPSGIDERTIARHLGEPTMPDVDLFVRSSGEQRTSNFLLWQSAYAEMVFLD
                     TLWPDFDRRHLWQAVQMYAARDRRYGGAIDVPEPAVHPVPAVHQEPLGL"
BASE COUNT          942 a         2532 c         2268 g         1125 t
ORIGIN      
        1 gtgatctcgt tgcggaagct cttgccgacc tgggcgatgc cgaacggcgg cttcttgcgg
       61 gccgcaccct cgacgttctt gaagttgatg aagatgccct gagccgtctc ggggcgcagg
      121 tagtgcagcc cgctctcgtc ctcgatcacg ccgaggtagg tcttgagcat catgttgaag
      181 tcgcgcgcct cggtccactg accgcggttg ccgcagtgcg ggcacgggac gtccgacatg
      241 ggaacgtcgt cggggttcat gcccccgccc ttcttggcgg catacgcctc ctgaaggtgg
      301 tcagcacgct cccgcttgtg gcacgagagg cattcgacga gcgggtcggt gaacgtgccg
      361 acgtgccccg aggcgaccca cacctggcgg ggcaggatga tcgaggagtc caggccgacg
      421 acgtcctcgc gagcgtggac catcgccttc caccactgcc gcttgatgtt ctccttgagc
      481 tcgacaccca acggcccgta gtcccaggcc gagcgcgtcc ctccgtagat ctccccgcac
      541 gggaaaacga agcccctgcg cttgcagagg ctgacgacgg tgtcgacggt ggaggtgctc
      601 gaagccatgc aggacaggtt atcggcccag cggcataccc cctgactggg tttcggcgcc
      661 gggtgtaggc cgccagcctt cggcaccggc gttcggcacc ggcgttcagc accgcgcaga
      721 cgctccctta ggctgtccgc cgtgagtctt tcgttccggt ccaaggttga gcacgcctcc
      781 gtccagtggg tgacgcggct caaccgggtt ccacggtggg cagccttcat cggcgtcctc
      841 gccctcatgg tcgccgggat cctcgtgccc aaggtcgggt tcctcttcac gctggtcatc
      901 gcggccttcc tcggctggtt gatcttcctc acctggccgc gtctggccgc ccccgagaag
      961 ctcatgcgca tcgcggtcct ggctttggtc gtcgccgtcg ccgtcatgca ggccttcccc
     1021 aaggcctgaa acccggtcag aaacccgtca gaccagcccg gcctcgcggg cggctcgacg
     1081 cacggcccca cgctcctcgt accgcgcccg ggtccccttg tgggcgaaac ccagccgctc
     1141 atagagcgcc cgcgccccgt tgtcgacgcc gacacacagc cacacccggc tcgccccggc
     1201 cttcttgagc gccgtcagcg agcggacgac gaggtgcgcc cccaaaccct ggccgcgcca
     1261 ttccggcacg acccccacct ggtcgatcca ctcgtccgac accgtgatga agcccgccgg
     1321 ctcgccgtcc tcggtcaacg cgacccgcga gtcctcgggg cggaaacccg agtcgtcgac
     1381 caaccagcgc agccactcct cctcgggcgg gtccgggaac cccggtcggt cgccgaagga
     1441 gctccggtat gccgcgtaga acgcgtgcgc cgtgtcgtcc cggaacggct cggtgtgcag
     1501 gccttcgggc agcgcgactc gggagatgtg cttgagtttg tgccgcataa ccgcctcggc
     1561 gaaggtgcgg tgcaggttgt tggcggcgaa caacgactcg gcctcgatcg acatcgtctc
     1621 ggccacgacc cggatcggct ggccgtcggc gtgcgcacga caccagtcga ccaacgcctc
     1681 cccatgacct tggcgccgca tcgacgggtg caccaggccg gtggcgacga cgcgtccggc
     1741 cgagtcgcga ctcatcgcag ccgcggcgac gatctcgccg gtctcgtcgc agccggtgac
     1801 gccttcaccc tgcaggaaca gcgcctggat ggtctcgtcg gtgcccagtt gcggcaaccc
     1861 gccgtcggcc tccagacact cctgggccag ggcaacgatc tggcccagat cgtcgctcgt
     1921 gagcgggcgc cactccagtg ctgacacagg cttcctcttc ccgctgttcc agtccggctg
     1981 cggcgggtcg ggcccaccgc gctcccacca tgagagcaca gggccctcta gcgcgtcatc
     2041 accgacccca agttgacaat gattctcatt tccgcgaccg tggaggtatg ccgcgcaccg
     2101 ctcccgcccg cccccgcccg accagccggg ccaaccggac acgtgtcatt cgcgcggccg
     2161 cttggggcgc ggtcgctgtc ctggcgttgg tgagcgcgtg cgcgtccccc cgggcggccc
     2221 aggagagcgc cgagtcggcc acggcacagg tccccgtctc cgcggcgttc tatccgctcg
     2281 cctacctcct cgagcaggtc ggcggtccag gggtgcgcgt ctggaccatc accaagccgg
     2341 gcgtcgagcc gcacgatctc gaactcaccc cgaaggacct cgtcgacctg cccaagatgt
     2401 cgctcgtcgc ctacctcaag ggcttccagc ccgccgtcga cgaggcggtc gcgcagcagg
     2461 cgccaagcgc ggcatacgac gtttccgccg cagccgacct gacgctcggc gcacccgcgg
     2521 gcgaggcgag cgcgaccgac ctgcacttct ggctcgaccc catccggtat gccgccgtcg
     2581 gcaccgccct cggggaacgc ctcgccctcg ccgaccccac ccatgccgcc gactaccgag
     2641 cccgcgccaa gacgttcgcg gcctcgatgg agcagttgga caagaccttc gcgaccggcc
     2701 tggcctcgtg tgccaccaag caactcgtca ccagccatgc cgctttcggc tacctcgccg
     2761 cgcgctacgg cctcacccag gtcccgatcg ccggcctgtc ccccgaccag gaaccgtccg
     2821 ccaaacagtt ggccgacgtc gctgcgacgg tcaaggcggc cggggtcaag acgatcttct
     2881 ccgagaccct cgtcgacccg aagttcgccc agaccatcgc ccaggagacg ggcgccacgc
     2941 tggccctgct cgacccggtt gaggggatca ccgaccagtc ggccgcgaag gactaccccg
     3001 gcgtcatgct cgccaacctc gcggccctgc gcaccggact gggctgctca tgagcacgcc
     3061 ccccgctccc cccacgcccg atccacccgc cacggctccc ccggtcatcg cgctgcgcgg
     3121 agcctccttc ggctacggcg atcacgccgt cgtccacgat gtcgacctca ccatcaccgc
     3181 cggtgaggtc gtcctcgtcc tgggccccaa cggctccggg aaatcgacaa tcgccaaagg
     3241 gctgttcggg ctcactgacc ttctcgccgg agaggtcgac gtcttcggta tgccgcgcgc
     3301 ggacttccgc gacttcccgc gcctcgggta cgtgccccag cggcataccc tcgcctcctc
     3361 agtgaccgtc acggtgtcgg aggtcgtgtc ctcgggccga ctgtcccggc agtcctggtt
     3421 gggcatcgcc acccgcgccg accggcagca ggtccgccgg gcgctcgaac tcgtcggcct
     3481 cgccgaccgg gccggcatcg atgtcggcgc cctgtccggg ggccaacagc gccgcgtgct
     3541 cattgcccgc gcattggccg gcgagcccga cgtcctcgtc atggacgaac cgacggccgg
     3601 ggtcgacatc gccagccagc acgggttggt cgaggtgctc gcgcggctcg tcgccgaggg
     3661 cctcaccctc gtcgtcgtca cccacgagtt ggccgccttc gccgacctgg tgacccgggt
     3721 cgtcgtcgtc gacgctgggc ggatcgcgtt cgacggcagc cgggacgagt tcgttgcggc
     3781 cggatcggac gtcctgcacg accacgactg gcatcaccac ctcgaccgcc ccgaccgtcg
     3841 gcctgcggtg atctccgcgg aaggccctct ctcccaggga ggttcgcgcg aatgagcgag
     3901 atgctgtcca tcgacttcat gcaacgggcc ctgctggctg ccctgctggt ggggttgtcc
     3961 gctcccctcg tcggcgtctt cctcgtccaa cggcgcctgg ccctcatcgg cgacgggatg
     4021 gggcatgtcg ccctcgccgg ggtggccgtc ggcgtgtggc tcggtcagtc acccgtgtgg
     4081 accgcgctca tcggcgcggt gctggccgcg atcatcgtcg agctcatccg cacatccggc
     4141 agcgcgcacg gtgatgtcgc gttggcgatc atcttctatg gcggcatcgc cgccagcgtc
     4201 gtcatcatcg ggaagtcccc caacggcacc ccagccagcc tcacggcata cctcttcgga
     4261 gcgatcacga cggtgacggc cgctgacctg tgggtcttcg gcgtgctgac ggtcgtcatc
     4321 ctcctcgtcg tcggggtcct gcggtggcgg ttgttcgcgg tcgccagcga cgaggagtac
     4381 gcccgatcgg ccggcctgcc cgtcctcgct ctcaacctcg cgctggcggt cctcaccgcg
     4441 gtcaccgtcg tcgtgtcgat gcggatcgtc ggcttgctac tcatcagcgc cttgatgatc
     4501 gtccccaacg cggccgccca actcctcggc cacagcttcc ggtcgtcgct cgcgtgggcc
     4561 gtggcgatcg gcgtcgcctg cgcggtcggc ggggtcgttg tgtcgtatgc cgccgacacc
     4621 ccctcgggcg gcaccatcgt gctgctggcc atcggggcgt tcgtcgtcgc ggcggtgggc
     4681 tcggcgctgc gcacccgact gggccgcgta tcgctggcgg ggcacgccga ggccgagggc
     4741 cacggccatg agcacggtcc cgggtgtggc catgaggtga tccagcacgg cgaccatgtc
     4801 gactatgtgc acgacggcca ccaccacgcg ccgcacggtg cccactacga cgaacacgcc
     4861 gagcacaccg accccgcaga acaccccgag cacacccagc aggccgaccg atgaccgcct
     4921 ccggtcggcg gccgacccgc caacaggctg cggtcgtggc ctacctcggc gagaccggcg
     4981 acttcgtgtc cgcccaggaa ctccatgccc ggatgcgcgc ggcgggcaac tcgatcgggc
     5041 ttgccacggt ctatcgctcg ctcgccgccc tcgccaccga aggcgcgctc gacgtgttgc
     5101 gcaccgacga cggcgaggcc gtctatcgcc agtgctcgac cgatcaccac caccacctcg
     5161 tctgccgcac gtgcggacgg accgtcgagg tcgccgggcc gacggtcgag acctgggccg
     5221 agcgggtggc gggcgaacac gggttccggg acgtgcggca taccgtcgag atcgtcggca
     5281 cctgcgccac ctgctgagag ccgcttctag actgcgctcg tgctcgaagc cgcgtggtgg
     5341 ggactcttcg gaggattggc gctggtcgcc gggtccctcg tcgggctcta cgggcgggtg
     5401 tcgacgcggg tgctcggcct cgtcatggcg ttcggcgcag gcgtgctcat cagcgcggtg
     5461 tccttcgaac tcacgctgga ggcgtttgag gtcggcggat cgagcgcggt cgtctggggc
     5521 ctgtctgcgg gcgcggcgac cttcttcgtc ggggactggc tcatcgacca acgcggtggc
     5581 catcggcgca agagcccggt gagccagacc cacgtcagcg agggtggggc gatcgccctc
     5641 gtgctcgggg cgctgctcga cggcatcccc gagtcggccg caatcggctc aagcctcttg
     5701 ggcggcggcg aggtgggtgt ggcgatggtg gccgcggtct tcctctcgaa cgtccccgag
     5761 tcgatgtcgg cgtccgccgg actcaaggcc gcgggtcgct cacctcgcta catcctgggc
     5821 ctctgggggc tggtgtgcct cgcgtcggcc gtcgccgccg ccctcgggta tgccgtgctc
     5881 ggcggtgcgt cgccagaggc cgtcgcgttc gtccagtgtt tcgcggccgg agcgatcatc
     5941 acgatgcttg gcgacacgat ggttcccgag gcgaccgagc acgccggacg gttggtcggc
     6001 ctgtttatcg tggcgggctt cacgctggcg ttcctgctct cccacgccgc ctgagcgctg
     6061 ctcagaggcc gagcggctcc tgatgcacgg caggcactgg atgcactgct ggctcgggga
     6121 cgtcgatcgc tcccccgtag cggcggtcgc gcgcggcata catctgcacg gcctgccaca
     6181 ggtgtcggcg gtcgaagtcg ggccacaggg tgtcgaggaa gaccatctcg gcataggccg
     6241 actgccagag caggaagttc gaggtgcgct gctcccccga cgatcgcaca aagaggtcaa
     6301 cgtcgggcat ggtcggctcg cccaggtgcc gggcaatggt gcgctcgtcg atccccgacg
     6361 gcttgagctt ccccgccgcg acctcctgag cgatgctgac gaccgcatcg gcgatctcgg
     6421 cccgaccgcc gtaattgacg cagaaataga gcgtgagccc ggtgttgtgc cgggtcatct
     6481 cctgagcgat ctcgagctcg ttgatcactg accgccacag tcgcggccgc cgacccaccc
     6541 agcgcatccg cacgccccag gagttgagct ggtcgcgtcg acgccggatg acatcgcggt
     6601 tgaagcccat gaggaagcgc acctcgtcgg gcgagcgccg ccagttctcg gtcgagaagg
     6661 cataggccga caggtgcggg atgcccagct cgatcgcccc cgcgacgaca tcgagcaggg
     6721 ccgcctcccc cgcctcgtga cccttggtgc gcggcagccc gcgttggttg gcccaccggc
     6781 cgttgccgtc catgacgatc gccacgtgct gcgggatgag gtcggcgggg atcgcgggcg
     6841 ggcgggctcc cgtggggtgc gggaagg
//
LOCUS       DAOMZY010000186         6859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_10369_length_6859_cov_6.406820, whole genome shotgun sequence.
ACCESSION   DAOMZY010000186 DAOMZY010000000
VERSION     DAOMZY010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6859
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10369_length_6859_cov_6.406820"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1380..1919
                     /locus_tag="PLK64_10800"
     CDS             1380..1919
                     /locus_tag="PLK64_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80279.1"
                     /translation="MVIVRATRKLLQRLGARPRIDIEQSTTLLGDWYATVLPWRPRQA
                     GLFVSERTLLPVLMPLAPAATLLDRFPAHLAQVLARHDVDAATVAKECIDTTDYQVAA
                     TASRSVVGSMNEFAFLAERYRQDAPDVDLTDLSVRLSSVPCGPLYPRHISPDRRTPSS
                     PQGLVDRCDAAAFGPLAAK"
     gene            complement(1999..2301)
                     /locus_tag="PLK64_10805"
     CDS             complement(1999..2301)
                     /locus_tag="PLK64_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80280.1"
                     /translation="MRATLHIEHPVRDFDAWKRVFDSFHDFRVAHDVRSVRVGRRVDA
                     ASHVAIDLVFDSEDAAAAFHRVLQEQVWSRQQATDLLAGGPSAAIHNLVEDTTISP"
     gene            2389..3336
                     /locus_tag="PLK64_10810"
     CDS             2389..3336
                     /locus_tag="PLK64_10810"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPV80281.1"
                     /translation="MDLAERLSALDVDGLTDRALRTAQLGLIAARVGFDAHVFAMTDP
                     ATGIVTSPHATVPMVAPGDLPAVIRRRYSTASVTQWRTVLAELGVTDTVSMTLQDRWG
                     RWGFLELWRCSAGFTDRERDRLAGLPPVLTASLRMAVARTFTVPDAPPTRLESGVILL
                     DDDLQVRSVTSGAGRALMRMLPPTEPGPPVPAAAYNVVAALLAHEDGIWPGEPWARVH
                     LGASRWASVRAERLEDRIAVSIGPSTTTERADLFARAHQLSPRQSEVLHLALHGMDTR
                     AIADELVIAHSTTEDHLKALLAKTDSTSRHQLLARALGG"
     gene            complement(3391..3876)
                     /locus_tag="PLK64_10815"
     CDS             complement(3391..3876)
                     /locus_tag="PLK64_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80282.1"
                     /translation="MLLSASDFEAEQEYHRQMRYLHNRLLHGYGTVLGLDVSVDDGTN
                     IHVSPGFAIDALGREIIVTEQMSLRLEPLGRDRRWVRDLAIAWREVPEDPAPSLDGPV
                     TFSRWIEEPELSLVLPDSPPTSEALILARLIGKARRGAVDVDATVRRHLGLPSEIPSA
                     E"
     gene            complement(4390..5433)
                     /locus_tag="PLK64_10820"
     CDS             complement(4390..5433)
                     /locus_tag="PLK64_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV80283.1"
                     /translation="MTSTQGPTGTTTTPTSATTTDRGVRRLISLVVGITAVIALMLLA
                     FAAPAINSGANDLPLAVSGPGPAVTQVTGALSSNRPGAFDVTTYPTAADAARAIGDRD
                     AIGGIAVAPTGITIQTAAGAGAPYAPLLRGIGTQLAASGQNVTYQEVAPLTIDDPTGA
                     GLTALGLPLIFGGLASAAALVLAYRGAVATRALTALALSVLAGFTVTAILQFGFGSFD
                     GSYWLTATAVSAGIAAISLTVLGLGLLLGPAGIGLGAVLMLFVANPLSGMATGPAWLP
                     QPWGQIGQYLPLGAAGTTIRSAAYFDGNGDTTAWIVLGCWALAGLLLALLASRRTATT
                     ALASTDEPGLRYA"
     gene            complement(5430..6125)
                     /locus_tag="PLK64_10825"
     CDS             complement(5430..6125)
                     /locus_tag="PLK64_10825"
                     /inference="COORDINATES: protein motif:HMM:NF037150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HPV80284.1"
                     /translation="MASKSPAAAGRTGRRRGQPHTRQQILDAARELFAERGYAGTSMR
                     AIGAAAGVDPALVHHYFGTKEELFRAVLDVPVDPEVLLRQIGGASGAETPARFVQTFL
                     NVWDSAETGPQMVSLARRVFADQRSTEMLRDFFGATVIGPATAALLVDTDRKDAEARV
                     ALVVSQMLGVVLLRRVVALEPLASMPVEQLTAALVPTIARYLDGALPARPTQDPPSTA
                     RPASNHRERKPTP"
BASE COUNT         1080 a         2318 c         2396 g         1065 t
ORIGIN      
        1 tgacccaacc attgcggcac agaccgcctt gaggtgtcct gcccgtcctc acgaggaaaa
       61 ccgctacctc gcataccgcc ttgttcagca gtctcctagc tgtgagaagg gtggccagac
      121 ctcagcgggc gttaggcggc gttgagacgc aggttcggcg tcgttcaagg gcagccgcgg
      181 ggcttcgcag cttcagcccg gccggtagac gccactgcat tggtcaatcc gcaggacggc
      241 gacctcccgg ctttcctcgt tgatgcggta cgaaacccgg caggtgcctt ggcgggcgga
      301 gtgagcgccg gccggttctc cgcggagcgg ctttcaggac gtcgcaccag ggtcagctgt
      361 cgctgatgtc atcctccgtc gacccgctgg gagccgcgct tggtccgggc tctcgcgtgt
      421 ctggatcttc tgccgcgtct tgaatgtcgt gcgggagagt agaagcttgg ggggcatgcg
      481 aattgtcggt ggagccctgc acgcccgcgg gcgagccgcc ctgtggcgcg ttgccgggca
      541 cgctgtcgtg aggtccccgg cgtgcgatct cccaggacag atggcggcgt gaaaggaact
      601 caaaaagcat ggccgcaacg acccaggcga cgaacagggc gaggaacgcc atgagaacga
      661 tcgaggcgac cagctcagcg ccctcctcgc ggaagagatc ggggtccagc cttgcggcga
      721 gagccagaac gcgacgaagg ccgtgagcga ggccgcaccg acgatcaccc gccccagagc
      781 cccgatgttc tgtacctcgg gccgaaggga ctccagtcct tggaccttgt tatggaactc
      841 cagaggattc agccacccat ccgggtccgc gggcacgacc tgaccctccg ccgcccggaa
      901 agttgagtcc gacagagtgg tgtacgacga cgtcggtgag cgtgtcctgc tgaggtaggc
      961 gcggtcaccg cgggatccaa ctgacggcgg tccacgctga ccacctgggc cctgtgatgc
     1021 ggcgggttcc agctcccgac ccactgagac ccactctcgc gcagcgcgta gagcctgtcc
     1081 gacagcctgt gcaagcctgg acgccgtctg cggtcacgcg acgattcctc gccgagcctt
     1141 tgctcatcga ccacccgacc agtgtggcgt cacccccagc gtcgtgtcga cgccccgtcg
     1201 cacccgagga gctccgtcac gccagaccgg agacccactg gggcccaacg ggtcaaggtc
     1261 aggctccgtg tcttggacgc cctgcaggcg cggcccggcc tcgacgtcct gcacgtacgc
     1321 ggctggcgga gccgcgcctg ctcagaactg cagacgagcc gacgcgagaa gatgaacgca
     1381 tggtcatcgt ccgggcaacg cgcaagctgc tccagcgcct gggcgcccgc cccaggatcg
     1441 acatcgagca gtcgaccacc ctactgggtg actggtacgc caccgtgctg ccgtggcggc
     1501 ctcgccaggc tgggcttttc gtcagcgaaa ggaccctgct gccagtgctg atgccactgg
     1561 ctcctgcagc cacccttctc gaccggttcc cagctcattt agctcaggtt ctggctcggc
     1621 acgatgtgga cgcggcaacg gtcgcaaagg agtgcatcga cacgactgac taccaagtcg
     1681 cagcgaccgc aagccgcagc gtcgtcgggt ccatgaacga gttcgccttc ctagccgagc
     1741 gataccgtca ggatgctccc gacgtagatt tgacggatct atcggttcgg ctgtcctcgg
     1801 tcccatgcgg cccgctatac cctcgccaca tcagccccga ccggagaact ccaagctctc
     1861 ctcaaggact tgtagaccgc tgcgatgccg ccgcgttcgg cccattggcg gcgaagtgac
     1921 gcaccagcct tggccttgag accggcaggg cgatcgagca ttcgtcaggg tgcgggccag
     1981 gcctgggccg ccaccctgtc agggcgagat ggtggtgtcc tccaccaggt tgtggatggc
     2041 tgctgagggg cccccggcca gcagatcggt ggcttgctgc cggctccaca cctgctcctg
     2101 caggacgcga tggaaggccg cggccgcgtc ctcgctgtcg aagaccaggt cgatcgccac
     2161 gtgcgacgcg gcgtcgaccc tgcggccgac ccggacggag cggacgtcgt gtgcgacccg
     2221 gaagtcgtgg aagctgtcga agacccgctt ccaggcgtcg aagtcgcgga ccgggtgttc
     2281 gatgtgcagg gtcgctctca tggccaccat cgtccagacc gggcggtgca ccctccatcc
     2341 ccgatctcgg ggatctgttc gcgacctcgc cggtcctacg ctgcaggggt ggacctcgcc
     2401 gagcggctga gcgcactgga cgttgacggg ctgacggacc gggcgcttcg caccgctcag
     2461 ctcggcctga tcgccgcccg ggtgggtttc gacgcccacg tgttcgccat gaccgacccc
     2521 gccaccggca tcgtcacctc cccgcacgcc accgttccga tggtcgcccc cggtgacctg
     2581 cccgctgtga tccggcgccg ctacagcacc gccagcgtca cccagtggcg cacggtcctg
     2641 gcggagctcg gggtcaccga tacggtgtcc atgacgttgc aggaccggtg gggacggtgg
     2701 gggttcctgg agctgtggcg ctgttcggcc ggtttcaccg atcgcgagcg tgaccggctc
     2761 gccgggctgc cccccgtgct gacagcctca ctgcggatgg cggtggcgcg cacgttcacc
     2821 gttcctgacg ctccccccac gcggctggag tccggggtga tcctgctcga cgacgacctt
     2881 caggtccgct cggtgacctc cggtgccggg cgcgccctga tgcgaatgct cccgcccaca
     2941 gagccgggcc cgccggtgcc ggccgccgcc tacaacgtgg tcgcggccct gctcgcccac
     3001 gaagacggca tctggcccgg tgagccctgg gcccgcgtgc acctcggggc aagcaggtgg
     3061 gccagcgttc gggccgagcg gctcgaagac cgcatcgcag tgtccatcgg cccctcgacc
     3121 accaccgagc gggcagacct gttcgcccgc gcccaccagc tcagcccccg acagagcgag
     3181 gtgctccacc ttgccctgca cggcatggac acccgcgcca tcgccgacga gctggtcatc
     3241 gcccactcca ccaccgagga ccacctcaag gcactgctgg caaagaccga ctccacctcc
     3301 cgccaccaac tcctcgcccg cgccctgggc gggtgaacac ccggcggtat gccgcggtcc
     3361 cggcagacgc gtgagcttcc cgcggcggtg tcactctgcg ctgggaatct cgctggggag
     3421 gccgaggtgg cgacgcaccg tggcgtcgac atcgaccgca ccgcgacgag ccttcccgat
     3481 caggcgtgcc aagatcagcg cctctgatgt gggagggcta tcgggcagga ccaaggagag
     3541 ttcgggttct tcgatccagc ggctgaacgt gacaggcccg tccaggctcg gcgcagggtc
     3601 ttccgggacc tcacgccacg caatagcgag gtcccggacc caccgccgat cgcggccaag
     3661 gggctccaga cgcagggaca tctgctcggt cacaatgatc tcccgaccga gtgcgtcgat
     3721 cgcgaacccg gggctgacgt ggatattcgt gccgtcgtcg acggagacgt ccagcccgag
     3781 caccgtgccg tatccgtgga ggaggcggtt gtggaggtac cgcatctgtc ggtgatactc
     3841 ctgctcggcc tcaaagtccg acgctgacag cagcatgccc acgctgtagt tcacacgacg
     3901 aggtggctcg ctcataccgc agtatccaat gaccctgcac actcgtcacc cagttcccgc
     3961 gttggagatg cgccgaaacg ccgcgcgcgt cacgccggtg atgtgacgca cgccttggcg
     4021 gcgctgtgac ccaagccctt ccgtctgcga cagagccatc cgcgtcaggg aggctgctgc
     4081 ggtcggggca gctgaccgag ctgcttgagc gtcgtcgggc gctgcgcgta ctgcgtcgcc
     4141 ttggccgatg cctggcactg attgaagttg atccgcggac tcggcgggcc gatcgtccgg
     4201 ggccggaggt cctcggcgag tcagcccaga accgtggggt cagcgatgcg gacctgtcga
     4261 ctgtcgtgat ccgaagccgg ctcactgcgg tccggtggac cagtgcggat gcgctgcgtc
     4321 gacgagtttg agatcaaggg acgccgcctt ggacctggta ggtagcgtcc gggttgcgca
     4381 tcggcgtgat caggcgtagc ggaggcctgg ctcatcggtg gaggcgaggg ctgtcgtcgc
     4441 tgtgcgccgt gaggctagga gtgcgagaag cagccccgcc aaggcccagc agccgaggac
     4501 gatccaggcg gtggtgtcgc cattgccgtc gaagtaggcg gctgaacgga tggtggtgcc
     4561 ggctgcgccg agcgggaggt actgcccgat ctggccccag ggctgcggta gccaggcggg
     4621 gccggtggcc atgccggaga gcgggttggc gacgaagagc atcaggacgg cgcccaggcc
     4681 gatgcccgct ggaccgagca gcagcccgag gccgagcacg gtcagtgaga tggcggcgat
     4741 tccggctgag actgcggttg cggtcagcca gtacgaaccg tcgaaggagc caaagccgaa
     4801 ctgcaggatg gcggtgacgg tgaagccggc gaggacggac agggcaaggg cggtgagcgc
     4861 gcgggtggcg accgcacctc ggtacgccag cacgagggca gcggcggagg ccaggccgcc
     4921 gaagatcagc ggcagaccca aggcggtgag gccggcgccg gtcgggtcgt cgatggtcag
     4981 gggggcgact tcctggtagg tgacgttctg tccggaggcg gcgagctggg tgccgatacc
     5041 tctcagcagc ggggcgtagg gggcgccggc accggccgcg gtctggatgg tgataccggt
     5101 gggggccacc gcgattccac cgatcgcgtc acggtcaccg atggcgcggg cggcgtccgc
     5161 ggcggtgggg taggtggtca cgtcgaaggc gcccgggcgg ttgctgctca gagcgccggt
     5221 cacctgggtg accgcgggcc cggggccgct gaccgcgagc ggaaggtcat tcgcgccgga
     5281 gttgatcgcc ggggctgcga aggcgagcag catcaacgcg atgacggcgg tgatgccgac
     5341 gaccagggag atcaggcgtc gaacgccccg gtcggtcgtc gtggcggacg tgggggtggt
     5401 ggtcgtgccg gtgggcccct gggtggaggt catggcgtcg gtttcctttc gcggtgattg
     5461 gaggcgggcc gtgccgtgga gggcgggtcc tgggtcgggc gagcgggcag ggcaccgtcg
     5521 aggtagcggg cgatggtcgg taccagtgcg gcggtgagct gctcgacggg catgctggcc
     5581 aggggctcca gcgccacgac ccggcgcagc aggaccacac cgagcatctg gctgaccacc
     5641 agggcgacgc gggcctcggc gtccttcctg tctgtgtcca ctaggagggc agcggtggca
     5701 gggccgatca cggtggcgcc gaagaagtcc ctcagcatct cggtcgagcg ctggtcggcg
     5761 aacacccgcc gggccaggct caccatctgg gggccagtct ccgcggagtc ccacacgttg
     5821 aggaaggtct gcacgaaccg agccggtgtc tccgcgccgc tggcaccccc gatctgccgc
     5881 agcaggacct cggggtccac ggggacgtcc agcactgccc gaaacagctc ttccttggtg
     5941 ccgaagtagt ggtgcacgag ggccgggtcc acgcccgctg cggcgccgat ggcccgcatc
     6001 gaggtgccgg cgtaaccgcg ttcagcgaac agctcgcgcg cggcgtccag gatctgctgg
     6061 cgtgtgtgcg gctgcccccg gcgccgcccg gtgcggccgg ctgccgccgg ggacttcgag
     6121 gccatggcca tctccgattt catcaactga tgaattcacg gtagaggacc gagcctggag
     6181 aagtcaacac ctgctgaatt tcagtgtcgg ggccagccag tgagaagtcg ccatctgagc
     6241 cgatcggcgg gctcgggagg ggccttgccc gcggcgccct cccctcgggt cccaccaacg
     6301 agcacggtcg aaagcaggac tcgccctgcc cagttaccat tcagtcccag ctgggccggc
     6361 gcccgatcct cgccgccggc aacacgggag gggacctgga gatgctcgag tacgccacgg
     6421 catccgacgg cccctccctg gccctcctcg tcgaccacga cgacgagtag cgcgagttcc
     6481 gctaacctcg gctcggccga gtcgttcacc gaccccgaac ccatcaccga cggtcgccgc
     6541 gcggctgggc tggacagtgg ccagcatgcg cgacgactgc gcccaggtct tcgcggaacc
     6601 ggagcacagc ccaagtatct gaagcatcgg aacgacaggc gcccggcgac ccactacaac
     6661 ttcgggcttg ggacccgtgc gctcttcggg gcggcgagtt gcgttcccca agtgggcgaa
     6721 gtagggcgcc cttagtgggc gcagtgacgc gtgcgcggcc cgtggtgccc acagccccac
     6781 tcttcatgat cgtcgtgaga gaccgcccct gtgcaatgtg caacgcccgg gctcgcgcaa
     6841 cctttgatgc atctcgaca
//
LOCUS       DAOMZY010000187         6858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_10371_length_6858_cov_5.671321, whole genome shotgun sequence.
ACCESSION   DAOMZY010000187 DAOMZY010000000
VERSION     DAOMZY010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6858
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10371_length_6858_cov_5.671321"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="PLK64_10830"
     CDS             <1..1175
                     /locus_tag="PLK64_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPV80285.1"
                     /translation="GERISLGDVTLQIMATPGHTPESISVLVFERADDDVAYGVLTGD
                     ALFIGDVGRPDLLASVGVSADALGRMLYDSVQHKLMGLADEVRVFPAHGAGSACGKNL
                     STEKQSTIGAQRALNYACQPMTEDEFLAVVTEGQPSAPAYFLYNATLNKQERATRGLD
                     AAIPALDDEAVAAALADGAVVHDARDAVAFTAGHLRGAVSVPADGRLSETAGMVFRPD
                     QPIVVMAPEGQEQEIALRLARIGFDRVVGYVADVEAYLVAHADQVTQASRLTVAQADT
                     ALTAQPTAQPAAGGTVAAGPAAAGPAAAGGVQLVDIRNPGETAGGTIPGARTIPLPEL
                     PRRLGELDATAPVIVYCAGAWRSSVAASLLRANGFVDVSDILGGYNAWRQAHPIAG"
     gene            complement(1236..1610)
                     /locus_tag="PLK64_10835"
     CDS             complement(1236..1610)
                     /locus_tag="PLK64_10835"
                     /inference="COORDINATES: protein motif:HMM:NF021525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HPV80286.1"
                     /translation="MSAWTASTLAAFAIVEEVRIVTQRQDGSLRRPRIIWVVALSDRL
                     FIRSVNGRDADWFRWAIATGTGQLEASGTAYDVTFTEAQEGDLDAVDAAYQGKYGRRY
                     ASIVRDLCRPVPRAATLQVHLA"
     gene            complement(1886..2320)
                     /locus_tag="PLK64_10840"
     CDS             complement(1886..2320)
                     /locus_tag="PLK64_10840"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV80287.1"
                     /translation="MTATLAGAVRVTVTEREARQAKDVLGTLGRPDGSLSVDLDGQRV
                     GPLPRDVGIVLQSVLQAMADGRTVTIGTIPEIVTTSTAASMLGISRPTLMKMIKDGEI
                     QAHKVGSHHRLRAEDVLAARRARRARERAAFDQLRELDDSTD"
     gene            complement(2615..4168)
                     /locus_tag="PLK64_10845"
     CDS             complement(2615..4168)
                     /locus_tag="PLK64_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HPV80288.1"
                     /translation="MTSPQLPRRSLLLGGLGVIGVGALAACTSGGAGHAGDAAHASNT
                     PHVGMDMGNGKTIGPVPSAGPSAGQKLVTATLTPKRTTLDLGGVTVDTWAYGDSVPGQ
                     AIRATAGDFLRVTVDNQLPADTTVHWHGIRLRNAADGVPGVTQDAIRPGARYVYEFTA
                     PDPGTFFFHPHVGLQLDRGLYAPLILDDPAEPGGYDAEWVVVLDDWLDGVGTTPDDVL
                     AKLIADGGPANGSGDMGGMGGMDHGGMGGMNHGMGQAPWGDAGDVTYPHYLINGKTPT
                     DPTTLSAKPGQRVRLRVINAASDSVFTVALGGHRMTLTHADGHPVQPTEVGAFYIGMG
                     ERYDAIVTLGDGAFPLVAAPFGKQGHALAVVRTGAGAAPGPDAMPSELGGQVFLGSRL
                     LPSAGSRLPDRAPDATVNLTLNGAMKPYLWGMNGAPYGKNSPLLARAGQRLRINVVNM
                     TMMSHPLHLHGPAFALADTGLRKDTLLLAPMESRQLDLDPEVGDWMVHCHNTYHAEAG
                     MMILLSATA"
     gene            complement(4173..4424)
                     /locus_tag="PLK64_10850"
     CDS             complement(4173..4424)
                     /locus_tag="PLK64_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HPV80289.1"
                     /translation="MMGWNGMGSGWMWLVMLAVIVGFWAVVVVLLRAVLRDRARDRDR
                     DALEPTLESPLVILDRRLVSGEIDIDEYVQLRRAITSGH"
     gene            4549..5253
                     /locus_tag="PLK64_10855"
     CDS             4549..5253
                     /locus_tag="PLK64_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPV80290.1"
                     /translation="MTASAGRVLVVEDEKDLAAMVGSYLQRAGYAVDLAFTGPDGVEA
                     ARSAEPDVVVLDLGLPGLDGIEVCRQLRTFTDCYVLMLTARTDEIDKIVGLSVGADDY
                     ITKPFSPRELVARVQVVLRRPRRPVVSGDDAQPPRVFGDLKVDLDGRQVRVAGHPVEL
                     TRTEFDILALLASSPKVAFSRRRILDEVWDPGWVGDEHVVDVHMAHLRRKLGDDPAEA
                     AYITTVRGVGYRMVTS"
     gene            5321..6433
                     /locus_tag="PLK64_10860"
     CDS             5321..6433
                     /locus_tag="PLK64_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPV80291.1"
                     /translation="MLAQGIVVGAGILTAALVAAIVGPQLFHEHLLQAGQEPNSPELM
                     HSEEAYASANTIALGAALLIALTAGFVVTWQLSRRMTQPLRDLATVATRLSNGDYSAR
                     APRPSAGPELDRLADTLNDVAERLESTEDTRRRLLADLAHEMRTPLAVIGAHLDGLED
                     GVTEWTPETARVLRDHTERLVRLSSDVAEVSRAEEGRLEERAIVEVSVGDLVRAAVEG
                     VRSEFAAKGVELVGAGGGAAQVTGATGVDVAAGLEVLVTVDRQRILQLLTNLLTNALR
                     HTPAGGTVRVDAQTSGEHVELTVTDTGDGISATHLPHVFERFYRGDTARDREHGGSGI
                     GLAIARGIAHAHRGTLTAYSAGPGMGATFTLTLPVG"
     gene            complement(6461..>6858)
                     /locus_tag="PLK64_10865"
     CDS             complement(6461..>6858)
                     /locus_tag="PLK64_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80292.1"
                     /translation="DEGYAVTPAGRIGAFATSTMTPHQALAILVALPEPKPPRIADED
                     QAWSLLEVQVAQPGGLAHREWSCHEVPDLGWLFVLLRVDVTVRFVVSRDGQIAATPRS
                     DETGAATLGPLIERQAHRIPVAHLVGPLT"
BASE COUNT         1034 a         2381 c         2377 g         1066 t
ORIGIN      
        1 acggagagcg gatcagcctc ggtgacgtga cgctgcagat catggcgacc cctggccaca
       61 cgcccgagtc gatcagcgtc ctcgtcttcg agcgcgccga cgacgacgtg gcctacgggg
      121 tgctcaccgg ggacgcgctg ttcatcggcg atgtcggccg gcccgacctg ctcgcgtcgg
      181 tcggggtaag cgccgacgcg ctcgggcgga tgctctacga ctcggtgcag cacaagctga
      241 tggggttggc cgatgaggtg cgggtgttcc cggcccacgg cgccggctcc gcgtgcggta
      301 agaacctctc caccgagaaa cagtcgacca tcggcgccca acgggcgctg aactacgcct
      361 gccaacccat gacggaggac gagttcctcg ccgtcgtcac cgagggccag ccgagcgctc
      421 ccgcctactt cctctacaac gcgacgctca acaagcagga gcgcgccacc cgcgggctcg
      481 acgccgcgat cccggccctc gatgatgagg ctgtggcggc cgctctggcc gatggagccg
      541 tggtgcacga tgcccgtgac gcggtcgctt tcacggccgg gcaccttcgt ggagcggtca
      601 gcgtgccggc cgacggtcgg ctgtccgaga ccgccggcat ggtgttccgg cccgatcagc
      661 cgatcgtcgt catggcgccc gagggtcagg agcaggagat cgcgttgcgc ctggcccgga
      721 tcggtttcga ccgcgtggtg ggctatgtcg ccgatgtcga ggcctacctg gtggcccacg
      781 ccgaccaggt cacgcaggcg agccggctca cggtcgccca ggccgacacg gctctgactg
      841 cccagccgac tgcccagccg gctgcggggg gaacagtcgc cgctggcccg gcggcggctg
      901 gcccggccgc agccggtggg gttcaactgg tcgacatccg caaccccggc gagacggccg
      961 gcggcacgat cccgggcgcc cggacgatcc cgttgcccga gctgcctcgg cggctgggtg
     1021 agctcgacgc gacggcgccc gtcatcgtct actgcgctgg cgcctggcgg tccagcgttg
     1081 cggcaagtct cttgcgcgcc aacggttttg tcgacgtgtc agacatcctt ggcggctaca
     1141 acgcgtggcg tcaggctcac cccatcgctg gctgagcccg gtccgtcggc tcagcatcgc
     1201 cacggatcga cgccggtgtc accgccggat ctcagtcagg cgaggtgcac ctgcagggtc
     1261 gctgccctcg gcacgggccg acacaagtcc ctcacgatgg aggcgtagcg acgcccatac
     1321 ttcccttggt atgccgcgtc gaccgcatcg aggtcgccct cctgggcctc ggtgaaggtc
     1381 acgtcgtatg ccgtgcccga cgcctcgagt tgcccggtcc ccgtggcgat cgcccatcgg
     1441 aaccagtcgg cgtcgcgccc gttgaccgac ctgatgaaca gccggtcgct cagcgccacc
     1501 acccagatga tccgcgggcg tcgcaggctt ccgtcctgtc gctgggtcac gatgcgcacc
     1561 tcttcaacga tggcgaacgc cgcgagcgtc gatgccgtcc acgctgacat gggacctcct
     1621 gctcgtccgg gcttgagctg cgggctgatc cacgagccta cgcggagcag ccttggcgcg
     1681 agtcgccctg tctggcggcc tggtcaagag gcgccgcgtg ggaccgggca tggggccgcg
     1741 catgaagccg ccgtaggtgt cgtgatcacg caccctacga cggcttcacg ggcgacttca
     1801 tccccggcat acccctgctg ccacctgaac agcgctcaaa cccacggggt atgccggcct
     1861 gcggcccgcc ccgtcggccg cacgttcagt cggtgctgtc gtccaactcg cgcaactggt
     1921 cgaaagcggc acgctcccgg gcacgacgcg ctctgcgtgc ggccaaaaca tcctcagccc
     1981 gaaggcggtg atgtgagcca accttgtgcg cctggatctc cccgtccttg atcatcttca
     2041 tcagtgtggg ccgcgagatg ccgagcatgc tggcagcggt cgacgtggtg acgatctccg
     2101 ggatagtgcc gatggtgacg gtgcgaccgt cagccatcgc ctgcaggacg ctctgtagga
     2161 cgatgccgac gtcgcgagga agcggcccca cacgctgccc atcgaggtcg acggacaacg
     2221 agccatcggg gcgtcccaaa gtgcccaaca catccttggc ctgccgtgcc tcccgctcag
     2281 tcacggtgac acgcacggcc ccggccaggg tcgcagtcat ggtgaacgct tcttcccctt
     2341 gcgtcttcca ggttgcttac acttagaagt gtaagtcgcc tgcggatata tgtgcaacct
     2401 cgcgaatttc agcggacggt cttgtgcgga gtcacggccc gggcgagagg ccgcgcatga
     2461 agccgccgta ggtgtcgtga tcacgcaccc tacgacggct tcacgggcga cttcatcccc
     2521 gcccgaggga ggcaagcaca cggcataccc ctggagtggt ggccgctccc gtaggcagga
     2581 ccggggagca gccggggtat gccgctcgct cgggtcaggc ggtcgcggag aggaggatca
     2641 tcatgccggc ctcggcgtga tacgtgttgt ggcagtggac catccaatcg ccgacctcgg
     2701 ggtcgaggtc gagctgacgg gactccatgg gggccagcag cagagtgtcc ttgcgcagcc
     2761 ccgtgtcggc gagcgcgaag gccggcccgt gcagatgcag ggggtggctc atcatcgtca
     2821 tgttgacgac gttgatccgc agccgttgcc cggcgcgggc gagcagcggg gagttcttgc
     2881 cgtagggcgc gccgttcatg ccccagagat agggcttcat ggccccgttg agcgtgaggt
     2941 tgaccgtggc gtcgggcgcg cggtcgggca gtcgggaccc cgccgaaggc agcagccgcg
     3001 acccgaggaa cacctggccg cccagttcgg acggcatcgc gtctggcccg ggagcggcgc
     3061 cggccccagt gcggaccacc gcgagcgcgt gcccctgctt gccgaacgga gcggccacga
     3121 ggggaaaggc gccgtcgccc agggtgacga tcgcgtcgta gcgctccccc atcccgatgt
     3181 agaacgcacc gacctcggtc ggctgcaccg ggtgcccgtc ggcatgggtg agggtcatcc
     3241 gatgcccgcc gagcgccacc gtgaagaccg agtcggacgc cgcattgatg acccgcaagc
     3301 gcacccgctg cccaggcttg gcgctcaacg tcgtcgggtc cgtgggggtc ttgccgttga
     3361 tgaggtagtg gggataggtc acgtcgccgg catcgcccca gggcgcctgg cccatgccgt
     3421 gattcatccc gcccatcccg ccatggtcca tcccgcccat gccgcccatg tcgccgctcc
     3481 cgttggccgg gccgccgtcc gcgatgagtt tggcaaggac gtcgtcgggg gtggtgccga
     3541 cgccatcgag ccaatcgtca agcacgacaa cccattcagc gtcatagcca cctggctcgg
     3601 ccgggtcgtc gaggatgagc ggcgcataga gcccacggtc gagttgcagg cccacatgcg
     3661 ggtggaagaa gaacgttccc gggtcggggg cggtgaactc atagacatag cgagcgcccg
     3721 gccggatcgc gtcctgcgtc actcccggga cgccgtcagc cgcattgcgc agtcgtatgc
     3781 cgtgccagtg caccgtggtg tccgcaggca attggttgtc gaccgtcact cgcaggaagt
     3841 cgcccgccgt ggcccgaatc gcttgtcccg gaacgctatc gccgtatgcc caggtgtcga
     3901 cggtgacccc gccgaggtcg agggtggtgc gcttgggggt gagggtagcc gtgaccaact
     3961 tctgtcccgc gctcggacca gccgacggca ccggcccgat cgtcttgccg ttacccatgt
     4021 ccatgccgac gtgcggcgtg ttgctggcgt gtgcggcgtc gccggcatgc ccggcgccgc
     4081 ccgaggtgca ggcggcaagc gcaccgacgc cgatcacacc cagacccccg agcaggaggg
     4141 atcgacgagg caactgtggg gaggtcatcg gctcagtgtc cactggtgat ggcccggcgc
     4201 aactgaacgt attcgtcaat gtcgatctcg ccgctgacca accgccgatc gaggatcacc
     4261 agcggcgatt cgagcgtggg ctccagcgcg tcgcggtctc ggtcgcgggc ccggtcgcgc
     4321 agcaccgcac gcagcaaaac gacgacaacc gcccaaaacc cgacgatgac ggccagcatc
     4381 accagccaca tccagcccga gcccatccca ttccaaccca tcatgggttg atgctcgcgc
     4441 ctgcgggtgt cggccgggcg ctgcgatacc tgaagatctc gtcaagatct gtccgcacgt
     4501 tgccgcaacg cgggccccca gcacgccacg cccgcgatga tgggtggcat gacagcgtcg
     4561 gccggccggg tgctcgtcgt cgaggatgag aaggacctcg cggcgatggt cggctcctac
     4621 ctccaacgcg ccgggtatgc cgtggacctc gccttcaccg gcccggacgg tgtcgaggcg
     4681 gcccgcagcg ccgagccgga cgtcgtcgtc ctcgacctgg gcctgcctgg cctggacggc
     4741 atcgaggtat gccgccaact gcgcaccttc accgactgct acgtgctcat gctgaccgcc
     4801 cgcaccgacg agatcgacaa gatcgtcggg ctgtcggtgg gcgccgacga ctacatcacc
     4861 aagccgttca gcccccgcga gttggtggct cgcgtccagg tggtcctgcg tcgaccccgt
     4921 cggccggtcg tgtccggcga cgacgcccag ccccccaggg ttttcggcga tctcaaggtt
     4981 gacctcgacg gtcgacaggt gcgggtcgcc ggccatcccg tcgagttgac ccgcaccgag
     5041 ttcgacatcc tcgccctgct cgccagctct ccgaaggtgg ccttcagccg ccgacggatc
     5101 ctcgacgagg tctgggatcc gggctgggtc ggcgacgagc acgtggtcga cgtccacatg
     5161 gcccatctgc gacgcaagtt gggagacgat ccggccgagg cggcatacat caccacggtc
     5221 cgaggcgtcg gctatcggat ggtgacgtcg tgaaccctgc gcgggtgacg cggtgaacct
     5281 ccgtgccgcg cgcgcgggct ggtcgctgcg cacgcggctc atgctggctc agggcatcgt
     5341 cgtcggcgcg ggcattctca ccgctgcgct cgtggcggcg atcgtcgggc cgcagttgtt
     5401 ccacgagcac ctgttgcagg cgggtcagga gcccaactcc cccgagctca tgcactcgga
     5461 ggaggcctac gcgagcgcca acaccatcgc gctcggcgcc gcactgctca tcgcgttgac
     5521 agcgggattc gtcgtcacct ggcagctgtc ccgccgaatg acccaaccgt tgcgcgacct
     5581 ggcgacggtg gccacccggc tgtcgaacgg tgactactcg gcgcgggctc cgcgtccgtc
     5641 ggccgggccc gagctcgacc ggctggccga cacgctcaac gacgtcgccg agcggttgga
     5701 gtcgaccgag gacacccgac gccggctgtt ggccgacctc gcccacgaga tgcgaacgcc
     5761 gctcgccgtc atcggtgctc acctggacgg gctcgaggac ggcgtcaccg agtggacgcc
     5821 cgagaccgct cgcgtgctgc gcgatcacac cgagcgactg gtgcggctgt ccagcgatgt
     5881 cgccgaggtg tcgcgcgccg aggagggccg cctcgaggag cgcgcgatcg tcgaggtctc
     5941 ggtgggcgac ctcgtgcgcg ctgccgtcga gggggtgcgg tcggagttcg cggccaaagg
     6001 ggtcgagttg gtgggtgccg gcggcggtgc tgcgcaggtg actggggcca cgggagttga
     6061 cgtcgcagcc ggactggagg tcctggtcac cgtggaccgc caacgcatcc tccaactgct
     6121 gacgaacctg ctcaccaacg cgctccggca cacgcccgcc ggtgggaccg tccgggtcga
     6181 cgcgcagaca tcgggcgagc acgtcgagct cacggtcacg gacaccggcg acggcatctc
     6241 cgccacgcac ctcccccacg tcttcgagcg cttctatcgc ggcgacacag cgcgcgaccg
     6301 cgaacacggc ggcagcggca tcgggctagc gatcgcccgc ggcatcgccc acgcgcaccg
     6361 ggggacgctc acggcataca gcgccggtcc ggggatgggc gcgaccttca ccctgacgct
     6421 tcccgtgggg tgacaccgcc gacgaggtat gccgtgcgcg tcacgtgagg gggccgacga
     6481 ggtgggcgac ggggatgcgg tgggcctggc gttcgatcag cgggccgagc gtggccgcgc
     6541 ccgtctcgtc gctgcggggg gtcgcggcga tctggccgtc cctgctgacg acgaacctga
     6601 cggtgacgtc cacgcgcagc aggacgaaga gccagccgag gtcgggcacc tcgtggcaac
     6661 tccattcccg gtgagcgagg ccgccgggct gggcgacctg gacctcgagc agggaccagg
     6721 cctgatcctc gtcggcgatc cgcgggggct tcggttcggg aagtgcaaca aggatcgcga
     6781 gtgcttggtg tggggtcatc gtcgaggtcg cgaaggcgcc gatcctgcct gcgggggtca
     6841 cggcataccc ctcgtcgg
//
LOCUS       DAOMZY010000188         6774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_10532_length_6774_cov_5.706206, whole genome shotgun sequence.
ACCESSION   DAOMZY010000188 DAOMZY010000000
VERSION     DAOMZY010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6774
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10532_length_6774_cov_5.706206"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="PLK64_10870"
     CDS             complement(<1..669)
                     /locus_tag="PLK64_10870"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPV80293.1"
                     /translation="MLQDLLGALGLASIYLLFALGMSLTWGTIDILNFSHGSIFMFSA
                     FTAHLLIPYVPMGFVGVALVCIVVGALMSLLVQVVAFEQIIKRARNRNTAERQILIGG
                     IGVAIIPLAIAQEVTKSNPFGFIDSGFEVTTWTFMGLRGTNILLITLTAAAALAVGTA
                     MWLRRSRHGLALRSIGVDSEVSAMMGVDRRRLAIVTMAVSGGLAGLAACLFTYTLGAI
                     TPESG"
     gene            complement(739..1935)
                     /locus_tag="PLK64_10875"
     CDS             complement(739..1935)
                     /locus_tag="PLK64_10875"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPV80294.1"
                     /translation="MDWTAVRLGRRGAVAVVAAAALGMSACSSSSGTSSTSAAAGGGL
                     PATINVVSINPQTGPAAFAGLAANKGYDLAIKEINDSKFLGSTTLTLSKVDTKGDIKT
                     AASSASTAIADKNVSAIFGSVSSQEAIAMSPLVQQAGLPTIYTQAGSDGVVVGDATYR
                     ATPLMRTYYSLVKKYVQEKGWKSVGIIHTAAAPTLVDIGTKALPAIATELGMTVTTTV
                     AVQGTTQDFAAPISQVLASKPDGVAILLVGAQNPTAMKQLRQAGYTGDVLGNSGASAG
                     NLKPAGADGAGMVWPVDFNFQQKAEASQKFVKAYQAANSGENPLNYAAEAYDAAWFLA
                     KSIKAAGSADRAKIKAAMATVASEKFDGALGNGLTWKDRDIVVPGVMIRWDGTGDVLL
                     YEGSAS"
     gene            complement(2095..3054)
                     /locus_tag="PLK64_10880"
     CDS             complement(2095..3054)
                     /locus_tag="PLK64_10880"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPV80295.1"
                     /translation="MLNAIPRLDLRDLQRARTERIHLARAGQAGWQPPEGVEISERQV
                     PAGSRPRVRVVTPTLAPSHPTDPTDPTDHPPRPALVWLHGGGHVMGTVWQDDPRLCRW
                     ALDTGAVVVAVDWRWAPEHPYPAALDDACAALRWCLAGADDLDIDPARVVVAGASSGG
                     GLAAGAALRCRDEGDPPLRAQVLIYPMLDDRGDTPSARTCTDPRVWNDESNSRAWAAY
                     LSGWSRPIPGYAAPARATDLTGLPPTWLATGALDLFRDEDLEYAARLLAAGVPTELHV
                     YAGAVHGFDLFAPNAGVSVRFRAELDAVMRAALTADTGWASLR"
     gene            complement(3147..3608)
                     /locus_tag="PLK64_10885"
     CDS             complement(3147..3608)
                     /locus_tag="PLK64_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HPV80296.1"
                     /translation="MAGLYFEDFEIGKEYVHPWSRTVTETDTILYCGLTMNPAPIHLD
                     AHYMETTIHGQRLVPSLYTGGLVGGMIVNELTLGTTLGNLGYTKFDFPKPVFHGDTIR
                     AETTILDKRESKSRTDSGIVFFEHRGINQRGEIVHIAHRAGLMLKRPVADG"
     gene            complement(3682..4059)
                     /locus_tag="PLK64_10890"
     CDS             complement(3682..4059)
                     /locus_tag="PLK64_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80297.1"
                     /translation="MVHRTKILLATRAVDRHPLTALWERIHDLDGLRDFSIRSRRMGF
                     RGQIVLHPSHVPVVNAVYTPDAEQIAFYRGLLETYEAAAARGDGAVMYGEVHVDKAHA
                     DKATEWLARVDTLASLNGGLPSA"
     gene            4114..4251
                     /locus_tag="PLK64_10895"
     CDS             4114..4251
                     /locus_tag="PLK64_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80298.1"
                     /translation="MIGPTRSEAAAISSQFWIPWMVSTGMMYSSPVTPARASKWSSSA
                     S"
     gene            complement(4601..5770)
                     /locus_tag="PLK64_10900"
     CDS             complement(4601..5770)
                     /locus_tag="PLK64_10900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPV80299.1"
                     /translation="MADYTPNPLHEDIRKAVREMMEPFDDQYWMEHDVDHAFPWEFYN
                     AVAKAGWLGITIPEEYGGGGLGVTEASIVEYEISASGGGMNACSAVHIGIFGFDPIIR
                     HGSEQLKQRFLPRVPTGDLHISFAVTEPDAGTDTTGITTFAKKVDGGFRVSGKKVWIT
                     KAQEAERMLVLVRTTKREDCVKRTDGMTLLFAEMDPNHVQVREIPKMGRNAVNTNEVF
                     IDDLFVADEDVVGEVGKGFRTLLTGLNAERIVAANAALGIGRAAVRRATAYANEREVF
                     GRPIGQNQAIAHPLAEAQMRLDAAELMVYKAAWLLDNGYECGPEANMGKFLAADAAFF
                     AADRALQTHGGFGYGKEFHVERYFREARLKRIAPISQEMVLNYLSEHVLGLPRSY"
     gene            complement(5796..6596)
                     /locus_tag="PLK64_10905"
     CDS             complement(5796..6596)
                     /locus_tag="PLK64_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPV80300.1"
                     /translation="MTQSTGSIRVSDRGGSRWVVIDNPAARNAYDQEMATRLAEVIEA
                     STDVRSVVMTGSPGAFCAGGMLAGLSAPTQHGMRELYRSSLRLFDAVRQCPRPVIAAV
                     NGAAAGGGNELVIACDLAIAAESATFGQTGPRVGSAPVTGATNVLGVQIGEKRAKEMA
                     MLCRRYTAAQALDLGLINAVVPDADLEATVQEWCEHIATLSPRYLEITKVSSNIWWNS
                     ARDSFAQGIGMLVQAIGSPDMVEGATAFLEKRRPDFRLDQGELPPDPR"
BASE COUNT         1002 a         2195 c         2461 g         1116 t
ORIGIN      
        1 gccgctctcg ggggtgatcg cgcccagggt gtaggtgaac aggcaggcgg ccagaccggc
       61 caagcctcca gacacggcca tcgtgacgat ggccagccga cggcggtcca cacccatcat
      121 cgccgagact tccgagtcga cgccgatgga gcgcagggcc agcccgtggc gcgaccggcg
      181 cagccacatc gcggtgccga cggccagggc cgcagctgcg gtgagggtga tgagaaggat
      241 gttggtgccg cgcagcccca tgaaggtcca ggtcgtcacc tcgaaccctg agtcgatgaa
      301 cccgaacggg ttgctcttgg tgacctcctg cgcgatggcc agcgggatga tcgccacgcc
      361 gatgccaccg atgaggatct gtcgctcggc cgtgttgcgg ttgcgggcgc gtttgatgat
      421 ctgttcaaag gcgaccactt ggaccaggag cgacatcagg gcgcccacga cgatgcagac
      481 gagcgcaacc ccgacgaagc ccatgggcac gtaggggatg agtaggtggg ccgtgaacgc
      541 ggagaacatg aagatcgagc cgtgggagaa gttgaggatg tcgatcgtgc cccacgtgag
      601 gctcatgccg agggcgaaga gcagatagat agacgccaac cccagcgcgc cgagcaggtc
      661 ttggagcacg gcaccgtcct ttcgtcccga agggacgggc ggccgcgggg gatgccccgc
      721 gaccgcccgt gtggatcctt agctggcgga gccttcgtag agaaggacgt cgccggtgcc
      781 atcccagcgg atcatgacgc cggggaccac gatgtcgcga tccttccagg tcagcccgtt
      841 gccgagggcg ccgtcgaact tctcgctggc caccgtggcc atggcggcct tgatcttggc
      901 acggtcggcg ctgccggcgg ccttgatcga cttggcgagg aaccaggcgg cgtcgtaggc
      961 ctcggccgcg tagttgagcg ggttctcgcc ggagttcgcc gcctggtagg ccttgacgaa
     1021 cttctgcgag gcctcggcct tctgctggaa gttgaagtcc accggccaaa ccatccccgc
     1081 accgtccgca ccggccggct tgaggttgcc cgcgctggcg cccgagttgc ccaggacgtc
     1141 accggtgtat ccggcctgac gcaactgctt catcgccgtg gggttctggg ccccgacaag
     1201 gaggatggcc acgccatcgg gcttgctggc gaggacctgc gagatcgggg ctgcgaagtc
     1261 ctgcgtggtg ccctgcacgg caacggtggt cgtcaccgtc atgccgagct ccgtggcgat
     1321 ggccggcagg gccttggtgc cgatgtcgac cagggtgggg gcggcggccg tgtggatgat
     1381 gcccacggac ttccaaccct tctcctggac gtacttcttc accagtgagt agtacgtgcg
     1441 catcaggggg gtggcgcggt aggtcgcgtc accgacgacg acgccgtcgc tgccggcctg
     1501 ggtgtagatg gtcggcaggc cggcctgttg cacgagcggc gacatggcga tcgcctcctg
     1561 gctcgacacc gagccgaaga tcgcgctgac gttcttgtcg gcgatcgccg ttgatgcgga
     1621 gctggcagcg gtcttgatgt cgcccttggt gtcgaccttg ctgagggtca gcgtggtgct
     1681 gcccaagaac ttgctgtcgt tgatctcctt gatggccagg tcgtaaccct tgttggcggc
     1741 cagtcccgcg aaggccgccg ggccggtctg cgggttgatc gagacgacgt tgatcgtggc
     1801 cgggaggcca ccgcccgccg ctgctgaagt cgagctcgtc cccgaagagc tcgaacaggc
     1861 cgacatgccc agagccgcgg cagcgactac tgctacggct ccccgtcggc cgagacgcac
     1921 agccgtccag tccatcgcgt cctcctttag aagatggtaa tcatgcacat gatccgcacg
     1981 atgcgctgac tctacgtccc gatgaccgac ctgtaaagga gttgccgtca atctgcgccg
     2041 ttccgtgatc cgggcggcat cctgccgagt gtgggtcgca gcccgccgaa ctggtcaccg
     2101 cagactggcc cagccggtgt ccgcggtgag ggcggcccgc atgacggcat cgagctcggc
     2161 ccggaaccgc acggagacac cagcattcgg cgcgaagaga tcgaagccgt ggacggcacc
     2221 ggcatacaca tgcagctcgg tgggcacccc ggcggcgagc aggcgcgcgg catactcaag
     2281 gtcctcgtcg cggaacagat cgagggcgcc ggtggccagc caggtgggcg gcaggccggt
     2341 gaggtccgtg gcgcgggcgg gcgcggcata cccggggatg ggtcgggacc accccgagag
     2401 gtatgccgcc cacgcgcggc tgttgctctc gtcgttccag acgcgggggt cggtgcacgt
     2461 gcgggccgag ggcgtgtcgc cgcggtcgtc gagcatcgga tagatgagga cctgggcgcg
     2521 caggggtggg tcgccctcgt ctcggcaacg cagagcggcg cccgcggcca gtccgccgcc
     2581 ggagctcgcg ccagcgacga cgacgcgggc cgggtcgatg tccaggtcgt cagcgccggc
     2641 gaggcaccag cgcaacgcgg cgcaagcgtc gtccagcgct gcgggataag ggtgttcggg
     2701 tgcccagcgc cagtcgacgg cgacgacgac tgcgccggtg tcgagggccc agcggcacaa
     2761 ccgcgggtcg tcctgccaga ccgtgcccat gacgtggcca ccgccatgca gccagacgag
     2821 cgcgggccga ggcgggtggt cggtggggtc ggtggggtcg gtggggtggg acggcgcgag
     2881 ggtgggcgtg acgacgcgga cccggggccg cgagccggcg ggcacctgcc gttcggagat
     2941 ctcgacgccc tcgggcggct gccacccggc ctgcccggcg cgtgcgaggt ggatgcgctc
     3001 ggtgcgggcg cgctgcaggt cacgcaggtc gagccggggg atggcgttga gcaccgcccc
     3061 gacctcaggg tcgagggggc ccggcagcag ggggtgctcc gtgggttggt cccagtcgct
     3121 cagtcgccag ccactcagcc ggtcgctcag ccgtcggcga cgggtcgctt gagcatgagg
     3181 ccggcgcggt gggcgatgtg gacgatctcg ccgcgctggt tgatgccgcg gtgctcgaag
     3241 aagacgatgc ccgagtcggt gcggctcttg gactcgcgct tgtccaggat ggtcgtctcg
     3301 gcgcggatcg tgtcgccgtg gaagaccggc ttggggaagt cgaacttcgt gtagccgagg
     3361 ttccccagcg tcgtgcccag ggtgagctcg ttgacgatca tgccgccgac caggccgccg
     3421 gtgtagaggc tggggacgag ccgctggccg tggatcgtcg tctccatgta gtgcgcgtct
     3481 aggtggatcg gcgcggggtt catggtgagc ccgcaataga ggatcgtgtc ggtttcggtg
     3541 actgtccgcg accacgggtg gacgtattcc ttaccgatct cgaagtcctc gaagtagagg
     3601 cccgccatga tggatctcct tgctgctctg ggggtttgac gctaggggtt taggcgctgg
     3661 gggtttgacg ttaggggtgg gtcaggcgga cgggaggccg ccgttgaggg aggcgagggt
     3721 gtcgacccgc gccagccact cggtggcctt gtcggcgtgc gccttgtcga cgtggacctc
     3781 gccgtacatc acggcgccgt cgccgcgcgc ggccgcggcc tcgtaggtct ccagcagccc
     3841 gcgatagaac gcgatctgct cggcatccgg ggtgtagacc gcgttgacga ccggcacatg
     3901 actggggtgc aggacgatct gcccgcggaa gcccattcgc cggctgcgga tgctgaagtc
     3961 gcgcagcccg tcgagatcat ggatgcgctc ccacagcgca gtgagcggat gccggtcgac
     4021 cgcgcgcgtg gccagcagga tcttggtgcg gtgcaccatc gactccagcc cctcgggcgt
     4081 ccactcgaag ccgaccgcct tggcgatgtc agcgtgatcg gccccacccg ctctgaggcc
     4141 gccgcgatct cctcgcagtt ctggatcccg tggatggtct ccacggggat gatgtattcc
     4201 agcccggtca ccccggcgcg cgcctcgaag tggtccagca gcgcgtcgta gcgcacgacg
     4261 tcgagcgccg agtcgacctt gggggcgaac acccccgtta gcccggggac gaccgccgcc
     4321 tcgaggtcca gcccggtcag ccgggtgccc agcgagttgg gtcgcacgaa cagtcccagc
     4381 gcgggatggg actgggagta tgccgcgacc gcctccgcca cctgctcccg ggcgccggcc
     4441 ttgaggtgct cggggaccga gtcctccagg tcgaagcaca cggcatcggg ccccgcgtcg
     4501 atcgccttgg cgaaccacga cgtcttgtgg gccggaacga acaggacgga acggtagggc
     4561 tgcacggtcg tggtctcctt cgactgccgc gaggggcaga tcagtaactg cgcggcagcc
     4621 cgaggacgtg ctcgctgagg taattgagca ccatttcctg gctgatcggg gcgatccgct
     4681 tgagccgggc ctcgcggaag tagcgctcga cgtggaactc cttgccatag ccgaacccgc
     4741 catgcgtctg cagggcccgg tcggcggcga agaaggccgc gtcggcggcg aggaacttgc
     4801 ccatgttggc ctcgggcccg cactcgtacc cgttgtccag caaccaggcc gccttgtaga
     4861 ccatcagctc ggcggcatcc aggcgcatct gcgcctcggc gagggggtgg gcgatcgcct
     4921 ggttctgccc gatcggccgt ccgaagacct cgcgttcgtt cgcgtatgcc gtcgcgcggc
     4981 gcacggccgc acgcccgatg ccgagggcgg cgttggcggc gacgatccgc tcggcgttga
     5041 gcccggtgag cagcgtccgg aagcccttgc cgacctcgcc gacgacgtcc tcgtcggcga
     5101 cgaaaaggtc gtcgatgaag acctcgttgg tgttgacggc gttgcggccc atcttgggga
     5161 tctcgcggac ctgcacgtgg ttggggtcca tctcggcgaa caacaacgtc atgccgtcgg
     5221 tgcgcttgac gcagtcctcg cgcttggtcg tgcggaccag cacgagcatc cgctcggcct
     5281 cctgcgcctt ggtgatccac accttcttgc cgctcacccg gaagccaccg tcgaccttct
     5341 tggcgaaggt ggtgatgccc gtggtgtcgg tgccggcgtc cggctcggtg accgcgaagg
     5401 agatgtgtag gtcgccggtc ggcacgcggg ggaggaagcg ctgctttaac tgctcggagc
     5461 cgtgccggat gatcgggtcg aagccgaaga tgccgatgtg cacggcgctg caggcgttca
     5521 tcccgccgcc ggaggccgag atctcgtatt cgacgatcga tgcctcggtg acgcccaggc
     5581 cgccgccgcc gtattcctcg gggatggtga tgccgagcca gcccgccttg gcgactgcgt
     5641 tgtagaactc ccacgggaag gcgtggtcga cgtcgtgttc catccagtac tggtcgtcga
     5701 agggctccat catctcccgg acggccttgc ggatgtcctc gtgcagaggg ttgggcgtgt
     5761 agtcggccat gacgtccttc gcggtggtgg gcgggtcagc gtgggtctgg ggggagctcg
     5821 ccctggtcca ggcggaagtc cgggcggcgc ttctcgagga aggccgtggc gccctcgacc
     5881 atgtcggggc tgccgatcgc ctgcacgagc atgccgatgc cctgggcgaa gctgtcgcgc
     5941 gcggagttcc accagatgtt cgagctgacc ttggtgatct ccaggtagcg ggggctgagc
     6001 gtcgcgatgt gctcgcacca ctcctgcacc gtcgcctcaa gatcggcgtc ggggacgacc
     6061 gcgttgatca gcccgaggtc cagcgcctgc gccgcggtgt aacgccggca cagcatcgcc
     6121 atctccttgg cgcgcttctc gccgatctgc acgccgagca cgttcgtcgc cccggtgacc
     6181 ggcgccgagc cgactcgcgg cccggtctgg ccgaaggtcg cgctctcggc cgcgatcgct
     6241 aggtcgcagg cgatgaccaa ctcgttgcct ccaccggccg cggcaccgtt aacggccgcg
     6301 atgaccgggc gcgggcactg gcgcacggca tcgaagagcc gcagcgacga ccgatagagc
     6361 tcacgcatcc cgtgctgggt cggcgcgctg agcccggcga gcatgccgcc ggcacagaac
     6421 gcgcccggcg agccggtcat gacgacggag cggacgtcgg tcgaggcttc gatcacctcg
     6481 gcgagacggg tcgccatctc ctggtcgtag gcgttgcgcg cggcagggtt gtcgatgacc
     6541 acccatcggc ttccgccccg atcggagacc cggatgcttc ccgtcgactg cgtcacggtc
     6601 tcataataat catatgcagc aatttggcca agggttgcgg gctcccgact tcgttgtcga
     6661 ggcctccgac gccctcggcg aaggcccctg ctgggaccct cgcgacgact cgcttggctg
     6721 ggtcgacatc cgcggccggg cggtcgcgct gaatgccggc ggtgccggca acgc
//
LOCUS       DAOMZY010000189         6766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_10540_length_6766_cov_6.000298, whole genome shotgun sequence.
ACCESSION   DAOMZY010000189 DAOMZY010000000
VERSION     DAOMZY010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6766
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10540_length_6766_cov_6.000298"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PLK64_10910"
     CDS             <1..540
                     /locus_tag="PLK64_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007270505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80301.1"
                     /translation="VEAPPLYASTMGVEGLFDRFHATEFAVPSHHTLHSGITGRGRPR
                     EEGYLIKVLNGITPIDEHTCWYYYAFCRNFGIENSAATEDLRIGLSTVLDEDAEALRL
                     QELALQTRPKGEHDVLIAQDAGVAKARRVMSQLLAAESVAAAGPASGTSEARYAASPA
                     AAAAAAPPAASASATASVG"
     gene            571..987
                     /locus_tag="PLK64_10915"
     CDS             571..987
                     /locus_tag="PLK64_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80302.1"
                     /translation="MTPAADGSVTLAACPDTADRGEDWRWRVWRGPPAAGSGGEVFVE
                     TDPDSVRATTSFVDAGPVTVSRRAGEEVVVVPLVGHHLVADSAGPWQRWLHPGDAFVL
                     EGEESETVNLSSAPGPARVAIVRLNTLNDAPLRWVP"
     gene            1023..1967
                     /locus_tag="PLK64_10920"
     CDS             1023..1967
                     /locus_tag="PLK64_10920"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPV80303.1"
                     /translation="MRPLMLVTLLATSSLALAACTNAGQASTSTTTAPAAGVAATSTT
                     AGSAAGKAALDPAVAALVPADIKAKGQLSFAMDASYAPFEYFDTDNKTIIGFDADLSQ
                     AIGTTMGIKVNDVNAAFDSILIGLTSQKYDVGMSAFSASAERAKVVDFVTYGAGGSGI
                     AVPKGNPKALSMDPMTLCGRTIAAQKGTIQGLDYLPKFSADCTAAGKGAITADLYPAQ
                     TDANLAVTSGRADAVMADSVSLALQAKATGTLELAAGPDYNPTNVAVAVPNDSPLAAP
                     IGAALKALDKNGTLAAIMAKWGLPTQFATQAKIGEIIK"
     gene            1964..>2856
                     /locus_tag="PLK64_10925"
     CDS             1964..>2856
                     /locus_tag="PLK64_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005269273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HPV80304.1"
                     /translation="MSAVPVLTPAEELTVVPKRHPGRWLAAAVILIIVVRMAISVLGN
                     ERFGWDTVNTYLRDVSIGSGLMMTLGLTVACMVVGVVLGALLAVMRLSANPVVSGAAW
                     AYVAAFRGTPVLVQLLFWYNLAALYPVITLGLPGVALDANQLISPTVAAILGLGLNEA
                     AYMSEIVRAGILSVDHGQQEAASALGLTRMETMRRIILPQAMRVIVPPTGNETIGMLK
                     TTSLVSVMAVHDLLYSAQIISARNYQIIPMLIVASIWYLVVTTILTIGQFYLERRFAR
                     GERTLPATPIQRLRRLLRTHAP"
     assembly_gap    2857..2956
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2957..3719
                     /locus_tag="PLK64_10930"
     CDS             <2957..3719
                     /locus_tag="PLK64_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HPV80305.1"
                     /translation="KSPMVIAERVHKKFGLNEVLKGVDLTIDRGEVVVMIGPSGSGKS
                     TFLRCINHLERVDAGRIWVDGQVVGYEQRGHHLHELSERAIARQRAHIGMVFQHFNLF
                     PHMTVLDNLIEAPRRVFGADREEVTARAEVLLGKVGLRDKIKAYPRHLSGGQQQRVAI
                     ARALCLQPSLMLFDEPTSALDPELVGDVLAVMKDLAASGMTMAVVTHEMGFARDVADR
                     VVFFDSGVIVESGPPDQVILNPQHERTRVFLRAVR"
     gene            3745..4707
                     /locus_tag="PLK64_10935"
     CDS             3745..4707
                     /locus_tag="PLK64_10935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005269275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HPV80306.1"
                     /translation="MPTAVESDLTLLVRQLRWESEGVVSVVLENADGSALPAWEPGAH
                     VDLHLPGSIVRQYSLCGSPDRSGQWRVGVLREESGRGGSAAVHEKVRPGTVIAARGPR
                     NNFVLRPSAAYVFIAGGIGITPILSMVAAADAAGAQWSLLYGGRTRPSMAFIDELAAY
                     GDHVLIHPQDELGLLPLGEMLRDPRPGTLVYCCGPEPLLRAAEEATAAWPAGSLVVER
                     FVPLPVDPAVSANDTTFTVEAAASGVTVAVPAGTTIVKALEAVGIFPPVSCEEGICGT
                     CETTVLGGIPDHRDSLLSEAEQAEGKTMMICVGRARGDRLVLDL"
     gene            4704..6026
                     /locus_tag="PLK64_10940"
     CDS             4704..6026
                     /locus_tag="PLK64_10940"
                     /inference="COORDINATES: protein motif:HMM:NF005913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPV80307.1"
                     /translation="MSGHPVVEPGPWWEGVAPLLSDLIRLDTQIGGPGERTAAEWVAG
                     RLTDDGLSPVLIESEPGRSTVVARVPGADPTLDPLLIHMHLDVVPAQAANWSVPPLSG
                     EIADGWVWGRGAVDMKNMVATVLTVVRRRLRTGHLPRRPLVLAFFADEENAGVLGAEW
                     AVATHPELFEGCRTAIGEGGGWSVPLAGGQRVYPLHVAEKGWAAMSVTATAPPAHASR
                     VYDDNPLRVLSEALGRLTHDRFPIHLTTQIEGLLRVAAEAAGTTFDPADPEASLHLLG
                     TEAANGAAALRHTAHATRLRAGVQSNVIPGDAWAELDCRYLPGRWDEFLGELHAALGD
                     RVQVRELARTDGLTPMTAGAGPAFFAAATQALRSQDAIASVAPIMLSGGTDATHLQRL
                     GIECFGFNPVLLPEGLDFWSLFHGVDERVPVSSLEFSARVLDDFLDRF"
     gene            complement(6210..>6766)
                     /locus_tag="PLK64_10945"
     CDS             complement(6210..>6766)
                     /locus_tag="PLK64_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPV80308.1"
                     /translation="PAGRWGLEGAEQGWAPDLFTFGKVIGGGLPLAAVAGPGALMDLL
                     APAGPVYQAGTLSGNPAASAAGLATLSLLDDAAYRRLDESAATVAAMVSTHLAAAGVP
                     HRVNAAGNLFSVFFTADPVTDYESAKRQDTAAYGRFFHELLDRGVWLPPSAYEAWFVS
                     TAHSDRELSVLQAALPAAARAAAG"
BASE COUNT          988 a         2375 c         2255 g         1048 t
ORIGIN      
        1 gtcgaggcgc ccccgctcta cgcctcgacg atgggcgtcg aggggctctt cgaccggttc
       61 cacgccaccg agttcgcggt tccgagccat cacaccctgc attcgggaat caccggacgg
      121 ggtcggccgc gcgaagaggg ctacctcatc aaggttctca acggcatcac gccgatcgac
      181 gagcacacct gctggtacta ctacgccttc tgccgcaact tcggcatcga gaactccgcg
      241 gcgaccgagg acctgaggat cggcttgagc accgtgctcg atgaggacgc cgaggcgctg
      301 cgactgcagg agttggcgct gcagacccgc cccaagggcg agcacgacgt cctcatcgcg
      361 caggacgccg gggtcgccaa ggcgcgccgc gtcatgtcgc agttgctcgc cgccgagtcc
      421 gtcgcagccg ccggccccgc gtcggggacc agcgaggcca ggtatgccgc gtcgccggcc
      481 gccgctgctg ccgccgctcc gcctgcggcc tcggcctcgg ccacggcctc ggtcggctga
      541 cctgtgtggc ttctcgtccg cgacaacgcc atgacgccgg cagccgacgg gtcggtcacc
      601 ctcgcagcgt gtcctgacac cgccgaccgc ggcgaggact ggcgctggcg ggtatggcgt
      661 ggtcctccgg cggcgggctc gggtggggag gtcttcgtcg agaccgatcc cgacagtgtc
      721 cgggccacca cgtcgttcgt tgacgccggt cccgtcaccg tcagccgacg tgccggtgag
      781 gaggttgtcg tcgtccccct ggtcggccac cacctcgtcg cggactcggc cggtccctgg
      841 cagcgctggc tgcaccccgg ggatgccttc gtcctcgagg gcgaggagtc agagaccgtc
      901 aacctctcca gtgcacctgg accggcccga gtggccatcg tccgcctcaa caccctgaac
      961 gacgcaccgc tgcgctgggt cccctgaccc agcggccaac ctccatcgaa aggtacgacc
     1021 cgatgcgccc gctcatgctt gtcacgctcc ttgccacctc atcccttgcc ctcgccgcct
     1081 gcacgaacgc gggtcaggcg tccaccagca ccacgacagc gcccgcggcc ggagtggccg
     1141 ccacatcgac cacggccggt tccgcggcgg gcaaggctgc cctcgacccg gcggtcgccg
     1201 ccctcgttcc ggctgacatc aaggccaagg gtcagctcag cttcgccatg gacgcgtcct
     1261 acgcgccgtt tgagtacttc gacaccgaca acaagacgat catcggtttc gacgccgacc
     1321 tgtcgcaggc catcggcacg acgatgggga tcaaggtcaa cgacgtcaac gccgcgttcg
     1381 actcgatcct catcgggctg accagccaga agtacgacgt gggcatgtcg gccttctcgg
     1441 ccagcgccga gcgcgccaag gtcgtggact tcgtgacgta tggcgccggt ggcagcggga
     1501 tcgccgtgcc gaagggaaac cccaaggcgc tgtcgatgga cccgatgacc ctgtgcggtc
     1561 gcacgatcgc ggctcagaag ggcaccatcc aggggctgga ctacctgccg aagttctcgg
     1621 cggattgcac ggccgcgggt aagggcgcca tcactgcgga cctctacccc gcgcagaccg
     1681 atgccaacct cgcggtgacc tcgggtcggg ctgacgctgt catggccgac agcgtgtccc
     1741 tggcgttgca ggccaaggcg accggcaccc tcgagctcgc ggccggaccc gactacaacc
     1801 cgaccaacgt cgcggtcgcc gtccccaacg actcgccgct ggccgccccg atcggggccg
     1861 ccctcaaggc cctcgacaag aacggcaccc tcgcggcgat catggccaag tggggcctgc
     1921 cgacccaatt cgccacccag gcgaagatcg gcgagatcat caaatgagtg ccgtgccggt
     1981 gctgacgccg gccgaggagc tgaccgtcgt cccgaagcgt cacccggggc ggtggttggc
     2041 cgcggcggtg atcctcatca tcgtggtccg gatggctatc tccgtcctgg gcaatgagcg
     2101 gttcggttgg gacacggtca acacgtacct gcgggacgtc tccatcggca gcgggctcat
     2161 gatgacgctc ggtctgaccg tggcgtgcat ggtggtcggc gtcgtcctcg gcgcgttgct
     2221 cgcggtcatg cggctgtcgg ccaacccggt cgtcagcggg gccgcgtggg cctacgtcgc
     2281 ggccttccga ggcacgcctg tcctcgtcca gttgctcttc tggtacaacc tcgcagctct
     2341 gtatcccgtg atcaccctcg ggctgccggg ggtcgcgctc gacgccaacc agttgatctc
     2401 gccgacggtg gcggcgatcc tcgggctggg actcaacgag gcggcataca tgtccgagat
     2461 cgtccgcgcg ggaatcctct cggtggacca cggacagcag gaggccgcga gcgctctcgg
     2521 tctcacccgg atggagacga tgcggcggat catccttccg caggccatgc gagtcatcgt
     2581 cccgccgacg ggcaacgaaa cgatcggcat gctcaagacg acctcgctcg tctcggtcat
     2641 ggcggtgcac gatctgctct actccgcgca gatcatctcg gcgcgcaact accagatcat
     2701 tccgatgctc atcgtcgcga gcatctggta tctcgtcgtg accaccatct tgaccatcgg
     2761 ccagttctac ctcgaacgca gattcgcccg aggtgaacgg accctgccgg cgaccccgat
     2821 ccaacggttg cggcgcctgc tgcgcacgca tgccccnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnncaag agccccatgg tcatcgccga gagggtgcac aagaagttcg
     3001 gcctcaacga ggtcctcaaa ggcgtggact tgacgatcga ccgaggtgag gtggtcgtca
     3061 tgatcggccc ctcggggtcg ggcaagagca ccttcctccg ctgcatcaac cacctcgaac
     3121 gggtcgacgc cggccggatc tgggttgacg ggcaggttgt cggctatgag cagcgcggcc
     3181 accacctgca cgagttgagc gagcgggcca tcgcccggca gcgggcacat atcgggatgg
     3241 tgttccaaca cttcaacctg ttcccgcaca tgacagtgct cgacaacctc atcgaggccc
     3301 ctcgtcgggt gttcggcgcc gaccgggagg aggtcactgc ccgagccgag gtgctcctcg
     3361 gcaaggtcgg tctgcgagac aagatcaagg cctaccccag gcacctctcc ggggggcagc
     3421 agcaacgcgt cgccattgcc cgagcgctgt gcctgcagcc gagcctcatg ctcttcgacg
     3481 agccgacctc ggccctggac cccgagctcg tcggggacgt cctggccgtc atgaaggacc
     3541 tcgcagcgag cgggatgacg atggccgtcg tcacccacga gatggggttc gcccgcgacg
     3601 tcgccgaccg ggtcgtgttc ttcgactccg gcgtcatcgt cgagtcgggg ccgccggacc
     3661 aggtcatcct caacccgcag cacgagcgga cccgcgtctt cctgcgcgcg gtccgctgag
     3721 tcaccgccgt ctgaaggagc cgccgtgccc accgccgtcg aatccgacct caccctcctg
     3781 gtgcgccaac ttcgttggga gagtgagggc gtcgtctccg tcgtcttgga gaatgccgac
     3841 gggtcggcct tgcccgcgtg ggagcctggc gcgcacgtcg acctgcactt gcccggctcg
     3901 atcgttcggc aatactcgct gtgcgggtcg ccggaccggt ccgggcaatg gcgggtcggg
     3961 gtgctgcgcg aggagagtgg gcgcggcggc tccgccgcgg tgcacgagaa ggtgcgcccc
     4021 ggcactgtca tcgccgcccg agggccgcgc aacaacttcg tcctgcgccc ctcggcggcc
     4081 tatgtgttca tcgccggtgg gatcggcatc accccgatcc tgtcgatggt cgcggcggca
     4141 gacgcggccg gcgcccagtg gtcgctgctt tatggcggcc gaacccgccc gtcgatggcg
     4201 ttcatcgacg agctcgcggc atacggagac cacgtcctga tccacccgca ggacgagctg
     4261 ggcctgctgc ccttgggtga gatgttgcgc gatcctcgac ccggcacctt ggtctactgc
     4321 tgcggacccg aaccgctcct gcgcgcggcg gaggaggcga ccgcggcctg gcctgcgggg
     4381 tcgctggtcg tagagcgttt cgttccgctg cccgtcgacc cggcggtgag cgcgaacgac
     4441 acaacgttca ccgtcgaggc ggcggcctcg ggtgtcacgg tcgcggtgcc ggccggaacg
     4501 acgatcgtca aggcgctcga ggcggtcggc atcttcccgc cggtgtcctg tgaagagggc
     4561 atctgcggaa cgtgcgagac gaccgtgctg ggcggcatac cggaccatcg cgactcgctg
     4621 ttgtccgaag cggaacaggc cgaggggaag accatgatga tctgcgtggg ccgcgctcgt
     4681 ggcgaccgat tggtgctgga cctgtgagcg gccacccggt ggtcgagcca gggccgtggt
     4741 gggagggcgt cgcgcccctg ttgtcggacc tcatccggct ggacacccag atcggcggtc
     4801 ccggtgagcg cacggcggcc gagtgggtgg ccggccggct caccgacgac gggctgtcgc
     4861 cggtgcttat cgagtccgag ccagggcgaa gcaccgtcgt tgccagggtg cccggtgccg
     4921 acccgaccct cgacccgttg ctcatccaca tgcacctcga cgtcgtcccg gcgcaggccg
     4981 cgaactggtc ggtgccgccc ctgtccggtg agatcgccga cggctgggtc tggggccgcg
     5041 gcgccgtcga catgaagaac atggtcgcca ccgtgctcac cgtcgtccgg cggcggctgc
     5101 ggaccggtca cctgccacgc cgtcccctgg tgctggcctt cttcgccgac gaggagaacg
     5161 ccggtgtgct gggggccgag tgggcggtgg cgacccaccc ggagctcttc gagggctgcc
     5221 gaaccgcgat cggcgaaggc ggcgggtgga gtgtgccgct cgccggcgga cagcgggtct
     5281 atccgctcca cgtcgccgaa aagggttggg ccgccatgtc ggtcacggca accgccccgc
     5341 cggcgcacgc gtcgcgggtc tacgacgaca acccgcttcg ggtgctcagc gaggcgttgg
     5401 ggcggctcac ccacgaccgt tttccgatcc acctcaccac ccagatcgag ggactgctgc
     5461 gcgtcgccgc cgaggccgcc ggaacaactt tcgacccggc cgatccggaa gcctccctgc
     5521 acctgctcgg cacggaggcc gccaatggcg ctgcggcgct gcggcatacg gcacatgcca
     5581 cccggctacg cgcgggcgtc cagagcaacg tcatcccggg tgacgcctgg gccgagctgg
     5641 actgccgcta tctgccgggg aggtgggacg agttcctcgg ggagttgcac gcggcgctcg
     5701 gcgaccgggt ccaggtgcgc gaactcgccc gcaccgacgg cttgacgccg atgacggcgg
     5761 gggccgggcc ggccttcttc gcggcggcga cccaggccct gcggtcacag gacgccatcg
     5821 cctcggtcgc gccgatcatg ctctccggcg gcaccgacgc gacccacctg cagcggctgg
     5881 gcatcgagtg cttcggcttc aatcccgtgc tgctgccgga ggggctggat ttctggtcgc
     5941 tcttccacgg cgtcgacgag cgggtgccgg tgtcgagcct ggagttctcc gcgcgggtgc
     6001 tcgacgactt cctcgaccgc ttctgagccg cgtgagcatg ctcctgtcgt gacgggccca
     6061 ggacccctgg agaaatcccg tgcctcaggt ccggactgcg tacctgaggc accggatttt
     6121 ccagccctcc gtgcgcctcg ggaggcggcg acgagcggcg agcagacctc agctcgccgc
     6181 tgaaccctct ggccgctgac ctgctcgcgt cagcccgccg cggctcgcgc agcggcaggc
     6241 agggccgcct gcagcaccga caactcgcgg tcggaatgcg cggtcgacac gaaccacgcc
     6301 tcgtaggcac tcggcggcag ccacaccccg cggtccaaca actcgtggaa gaaccgcccg
     6361 taggccgcgg tgtcctggcg cttggctgac tcatagtcag tcaccgggtc ggcggtgaag
     6421 aacacactga agaggttccc cgccgcgttg acccgatgcg ggaccccggc cgcggccagg
     6481 tgggtgctca ccatcgccgc caccgtcgcg gccgactcgt ccaggcgcct gtatgccgcg
     6541 tcgtccagga ggctgagcgt ggccagtccc gctgctgacg ccgcagggtt ccctgagagg
     6601 gtgcccgcct gatagaccgg tccggccggg gcgagcaggt ccatcagggc acccggcccg
     6661 gccactgcgg cgaggggcag cccaccaccg atgaccttgc cgaacgtgaa caggtctggt
     6721 gcccaaccct gttcggcacc ttcgagcccc cagcgaccgg ccggtc
//
LOCUS       DAOMZY010000190         6718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_10636_length_6718_cov_9.677172, whole genome shotgun sequence.
ACCESSION   DAOMZY010000190 DAOMZY010000000
VERSION     DAOMZY010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6718
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10636_length_6718_cov_9.677172"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PLK64_10950"
     CDS             <1..221
                     /locus_tag="PLK64_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80309.1"
                     /translation="PVPTEDLVDELWNGSPPATARTAIRVHVERTRAEGVEVPEDQRT
                     LTSSAHEDLDDARAHGVLPRARRLLHAL"
     gene            657..1193
                     /locus_tag="PLK64_10955"
     CDS             657..1193
                     /locus_tag="PLK64_10955"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV80310.1"
                     /translation="MPTTHPSAPSVRPLRAGEVEALIEVFDGLSAQSRFLRFNAPLPR
                     LTTLMVEQLTAQEEGRHEAFVASVDGMAVGIGRWVRYAAHPQRADVAVEVVDAYHRRG
                     IGRALMQAVTESAALAGVEFLFFCVRRDNDPARKPLAEAGVLTNVDDPEQLWIPVSRL
                     LAHLRTREPADGTLVRAG"
     gene            complement(1235..4072)
                     /locus_tag="PLK64_10960"
     CDS             complement(1235..4072)
                     /locus_tag="PLK64_10960"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HPV80311.1"
                     /translation="MLAVLVQRRGRSVPPEVLLDLVWGDAALGLNPTAVHTVVARLRR
                     QLSAPDLVRTGREGYLLPLEVDVDAVDHEHLIRSARDLRSAGQVDEAVRRYRRALALW
                     SGPLAYEGVTDELVLAERTRLEDAKATANEQLATLLLEGGTVPHLEEALAVAKDLVGE
                     FPLREGPTRLVMRAAYALGSQAEALRAYADLRARLREELGIDPSPPTQALHVQVLAQA
                     ESLVAPARPEHRRITTAIPVPASETIGREEDLERLRRWREAGHRLLTLVGPGGVGKSR
                     LLHELGREVSGELVYVDLSGLTEASAQEVAAAICIEYRLGAAGPDPVATLSAALTRAH
                     LTVLVDEAECSSTAVATIARVLMEHCPGVSMVITSRTPLGVPGERLVPVELLDCPQSG
                     EAGALARTLPAVRLLEARLTDRAPGLLITDDDGDLLGQIVRDVDGLPLGIELIAGQAA
                     TRSLTELSELTSRRLDVEDDRATGTDRHRSLRVLAQSGIDRLGPAERATFRGVGVFRG
                     SFTADAARVVVGDAATDHILRALARDAIIQLDRRGPRVRFRLLRTMRDLALELVAETG
                     PDELARLQSRHREWYADRWRGVPFHDEMLNEVQDGFEDYLQALENALTCRDAHSVAGL
                     GVTLGWYWLFHEAATTPWLDRILRSSLLSPADHAKIALMQRSLDESLTPHATAPDAAT
                     TKALLADDPEWAVLAGLVESIGAYVVGDVDTALLEAEALLEPAATTAAHALPEVLATV
                     AVMRATAGRHDAALTAAEEAWHLIGSSPGVIQLSAVVAKIGLALLDAGHPSRALAILT
                     KAIDDADRRLGVEPSGTLLLNAGWAALDCEVPEQAAYWFDRALNESPRVVQFFLAEAA
                     SGASATLCRLGVPGARGHSAAAAELCRRQGVPVFPRLTAAVDAAAASTGGADGDLEGG
                     QDVSRWVAVRDDELIAPICEAIQAVRQRTRP"
     gene            complement(4441..5523)
                     /locus_tag="PLK64_10965"
     CDS             complement(4441..5523)
                     /locus_tag="PLK64_10965"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HPV80312.1"
                     /translation="MLRTRPVVVPGLTCAATQVVYTTAATNSRTIAVSGTVLVPAMSW
                     RGPGPRPVLSYGVGVHGLGRDSAPSHLLATGLEGELAFLELALSRGWSVVVTDGEGLG
                     LPGPHTYGAGRVGGRAMLDIVRAAVAGVDGVEVGAPVLLWGYSEGGRCAAWAAEHQPI
                     YARELTLVALAAGGVPTDLAAVVEAIDGGPYSGLGLAVLVGLAHAHEDPRLWDILNAR
                     GRAAAAVAATLDVTGLVVSHPEPMAAWTTRERPWEDPLWAALLRAERNPGGTPEVPVY
                     LYHAKGDDIVPAELSRQLAAAYEAMDVHVTHVELDSGDHLTGAVDGADAAITWLAEQL
                     DAHLDLHRDPLAGPDAADELMARSTA"
     gene            5761..5925
                     /locus_tag="PLK64_10970"
     CDS             5761..5925
                     /locus_tag="PLK64_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80313.1"
                     /translation="MINTQTRHRILRIATLGGVGLTVVASGLAIRAVRSLDEAFTSAI
                     SDPYLATEES"
BASE COUNT         1070 a         2263 c         2343 g         1042 t
ORIGIN      
        1 gtccggtccc gaccgaggac ctggttgacg agctgtggaa cggcagtcct cccgccaccg
       61 cccgcaccgc gatccgggtc cacgtcgagc gcacgagggc cgaaggggtc gaggtccccg
      121 aggatcagag aactctcaca tcttctgctc acgaggacct cgacgatgcc cgagcccacg
      181 gtgtgctgcc gcgtgcgcgg cggctactgc acgcgttgtg atctgctggt tggcctgcct
      241 gggctgcatt gatcaagcgc ggattcgggc agatcgcacg cctgcccagc acgcgatacc
      301 gagctcgatg cacaggcccg gacggaagcc tccataacgt cccgccccaa ggcagggtgc
      361 ataagagcgt tcttcgttta ccgcatagcg gagatagcgc cttccgaccg ctcgggggat
      421 ttccaccact tcaccgaaag ggccgatctc tgcgacgtgg tcgttacgag accatgcagt
      481 cttcgctgca tcgcgacaac ggggaattgc cgcatgactg gatgcgatcg acacggcagt
      541 tttcgacctg gaaaccagtg aacgcgcggt gcggtggagc gcagtgtcag gtctgcgaca
      601 ggctggcgac agaccgtgga gcaaccctgt gggcaatcgt tcatcggagg aacgtcatgc
      661 ccaccaccca ccccagtgca ccgtcggtgc gaccactgcg agccggcgag gtcgaagcgc
      721 tgatcgaggt cttcgacggg ctctcagccc aatctcgctt cctgaggttc aacgcgccgt
      781 tgccacgcct caccacgttg atggtcgagc agttgaccgc gcaggaagag ggtcgccacg
      841 aggcgttcgt cgcgtctgtg gacggcatgg ccgtgggtat cggtcgctgg gtcaggtatg
      901 ccgcgcaccc ccagcgcgcg gatgtcgccg tcgaggtcgt ggacgcgtat caccggcgcg
      961 gcatcggtcg cgcgctgatg caggccgtca cggaaagcgc tgccctggcc ggtgtcgagt
     1021 tcctcttctt ctgcgtccga cgtgacaacg accccgcgcg aaagccgttg gcagaagctg
     1081 gagtcctcac caacgtcgac gacccggaac aattgtggat cccggtctcg cggctgctcg
     1141 cccacctgcg tacgcgagag ccggccgacg gcacccttgt tcgggcaggc tgattggggc
     1201 cggttcggcg gccacgaccc ggccctgact cgaatcaggg ccgggtccgt tgccggaccg
     1261 cttggatcgc ctcacagatc ggggcgatga gttcgtcgtc acgcacggcg acccagcgac
     1321 tcacgtcttg acctccctcc aggtctccgt ccgcaccccc tgtcgacgcg gcggcggcat
     1381 cgacggctgc cgtcagccgg ggaaagacgg gtacaccttg ccggcggcac aactcagcgg
     1441 cggctgcact gtgcccgcgc gcgcccggga cgcccaatcg gcacagggtg gccgatgccc
     1501 cggatgctgc ctcggcgagg aagaactgca cgacgcgcgg gctctcgttg agtgcgcggt
     1561 cgaaccaata tgccgcttgc tcggggacct cgcagtccag ggctgcccag ccggcgttga
     1621 gaagcaaggt gccgctgggc tcgactccca accgccgatc ggcgtcgtcg atggccttgg
     1681 tgaggatcgc gagcgcccgt gatggatgcc cggcgtcgag cagggcgaga ccgatcttgg
     1741 cgaccacggc actgagttga atgacgccgg gcgaggagcc gatgaggtgc caagcctcct
     1801 cggccgccgt cagcgcagcg tcgtgccgtc cagcagtggc tctcatgacg gccacggtgg
     1861 cgaggacttc gggtagggcg tgggcggcgg tggtagccgc gggttcgagg agagcctcag
     1921 cctcgaggag cgcagtgtcg acgtcgccga cgacgtacgc gccgatgctc tcgacgagac
     1981 cggccagaac ggcccactcg gggtcgtcgg cgagtagggc cttcgtcgtt gctgcgtcgg
     2041 gcgccgtggc gtgtggcgtc agcgattcat cgagggagcg ctgcatcagc gcgatcttgg
     2101 cgtggtcggc gggactgagc aggctcgagc gcaggatgcg atccagccag ggcgtggtcg
     2161 ctgcctcgtg gaacagccag taccagccca gggttacccc gagcccggcc acgctatgtg
     2221 catcacggca cgtgagagcg ttctccaggg cttggaggta gtcctcgaat ccgtcctgga
     2281 cctcgttgag catctcgtcg tggaagggga cacctcgcca gcgatcggca taccactctc
     2341 gatggcgaga ttgcagacgg gcgagctcgt ccggaccggt ctcggcgaca agttcgagcg
     2401 ccaggtcgcg catggtgcga agcaggcgga accgcacccg agggccgcgc cggtccaact
     2461 ggatgatggc gtcgcgggcc agcgcccgca ggatgtggtc ggtggcggcg tctccgacca
     2521 cgactcgtgc ggcgtccgcg gtgaaggatc cgcggaagac gccgacgcca cgaaaggtgg
     2581 cgcgctcggc aggcccgagt cggtcgatcc cggactgcgc gagcacgcgc agtgagcgat
     2641 gccgatcagt gcccgtggcc cggtcgtcct cgacgtcgag gcggcgcgag gtgagctcac
     2701 tgagttcggt caaagagcgg gtcgcggcct gtccggcgat gagttcgatg ccgagcggga
     2761 gtccgtcgac gtcacggacg atctggccga gcaggtcgcc gtcgtcgtcg gtgatgagga
     2821 gcccgggcgc gcgatcggtc aagcgtgcct ccagcaagcg aacggcgggt agggtccggg
     2881 ccagggcacc tgcctcgccc gattgagggc agtcaaggag ttcgacgggt acgagccgtt
     2941 cgcccggcac tcccagcggg gtgcgtgagg tgatgaccat ggacacgccg gggcaatgct
     3001 ccatgagcac ccgtgcgatc gtcgccacgg ccgtggacga gcactccgcc tcgtcgacga
     3061 gcacggtgag gtgggcccgg gtcagcgcgg cgctcaaggt ggcgaccggg tccgggccgg
     3121 ccgctccgag gcggtactcg atgcagatgg ccgcggcgac ctcctgcgcg gaggcttccg
     3181 tgagtccgga caggtcgacg tagaccaact ccccactgac ctcacggcca agttcgtgca
     3241 gcaaccgact cttgcccact ccgcccggac cgacgagggt gagcaaccgg tggcccgcct
     3301 cccgccacct ccggagccgt tccaggtcct cctcccgccc gatggtctcg gacgccggca
     3361 ccgggatcgc ggtggtgatc cggcggtgct cggggcgagc gggcgccacg agtgactcgg
     3421 cctgggccag cacctggaca tgcagggcct gcgtcggggg gctgggatcg atgcccaact
     3481 cctcccggag ccgggcgcgc aggtcggcat aggcgcgcag cgcctccgct tggctcccca
     3541 gtgcgtacgc cgctcgcatg accaacctgg tgggcccctc ccgcagcggg aactcaccga
     3601 ccaggtcttt cgcgaccgcc agcgcctcct caagatgcgg gaccgtcccg ccctcgagca
     3661 ggagggtggc cagctgctcg ttcgcggtgg ccttggcgtc ctcgaggcgc gtccgctccg
     3721 ccaggaccag ctcatccgtg acaccttcgt aggccagagg cccagaccac agggccagcg
     3781 cacgacggta ccgacggacg gcttcatcga cctgcccggc cgagcgcagg tcacgggcgc
     3841 tcctgatcag gtgctcgtgg tcgaccgcgt cgacatcgac ctccagcggg aggaggtagc
     3901 cctccctacc ggtccggacg aggtcggggg ccgacagttg ccgacgcagg cgagcgacga
     3961 cggtgtggac cgcggtcggg ttgagaccca aggcagcgtc gccccagacc aggtccagga
     4021 gaacctcggg tgggacgctg cgcccgcgcc gttggacaag taccgcgagc acgtccctgg
     4081 gtcgctgacc ggtcagtggt ccccggccag ctggggtctc gacctcgacc gggccgagga
     4141 ctcgaatccg cacccttcca tggaaccacc cgactagggc ccacggctac cgggttgtca
     4201 gccccgcacg gtcgccagga cctggatgta ccccaaccga gggtgcgcgg tgggccttcg
     4261 aagacccgcg cccccatgcg tcgaccccgc actcctgctc gggtggatgc acgaccggat
     4321 cgacaccagc tcattccggg gactacggca cgttgtgcgg gtcgcgttca ccgacggtcc
     4381 acgcccggaa tggatcgtga tcgagcgcgg aatcgcctcg gtgtgcccga cagacccggg
     4441 ttatgccgtg gacctcgcca tcagctcgtc ggccgcgtct ggcccggcca atggatcgcg
     4501 gtgaaggtcg agatgtgcgt cgagctgctc ggccagccag gtgatcgctg cgtcggcccc
     4561 gtcaacggca ccggtgaggt gatccccgga gtccagctcg acatgggtca cgtggacgtc
     4621 catggcttcg taggccgcgg cgagctgacg actcaactcg gcgggcacga tgtcgtcacc
     4681 ctttgcgtgg tagaggtaaa ccggcacctc gggggtgccg ccggggttgc gttcagcccg
     4741 caggagcgct gcccagagcg ggtcctccca cggacgctca cgcgtcgtcc aggccgccat
     4801 cggttccggg tgggacacca cgagtcccgt cacgtcgagg gtggcggcca ccgcggcggc
     4861 tgctcggccc cgcgcgttga gtatgtccca cagccgaggg tcctcgtgag cgtgggccag
     4921 gcccacgagg acggccaggc cgaggccgga gtaggggcca ccgtcgatcg cctcgacgac
     4981 ggctgcgagg tcggtcggga ctccaccggc cgcgagggcg accaaggtca gttcgcgcgc
     5041 atagatgggc tgatgctcgg cggcccacgc tgcgcagcga cctccttcgg agtaccccca
     5101 gagcagcacg ggagcgccga cctccactcc gtccacgccg gcgacggctg cccggacgat
     5161 gtcgagcatc gcccgccccc cgacgcggcc agctccgtag gtgtgggggc cgggtaggcc
     5221 gaggccctca ccatcggtga cgacgacgga ccagccgcgg ctgagggcaa gctccaggaa
     5281 ggcgagttcg ccctccaggc cggtcgcgag caagtggctg ggtgcgctgt cgcgtccgag
     5341 cccgtggacg ccgaccccgt acgagaggac cggccgaggt cccggtccac gccacgacat
     5401 ggcgggaacg aggacggtcc cggagacggc gatcgttctg ctgttggtcg ctgccgtggt
     5461 gtagacaacc tgggtggccg cgcaggtcag ccccggcacg acgactggcc gggtacggag
     5521 catggttcct ccgcactggg gcatcgaggc gggtgtgtcg tagaaggggt ccgtcgaggg
     5581 ggcgtgcaca acgctgtcct ttcgtcgagg tgctcttgac ctcaggcagc atcccgaacc
     5641 aggccgtcac cgatctgtca cgcacggccg accggctgac agatcggtga cagggccctc
     5701 gctcacgctg tgacctggtc ggcccccaac gaatcggcca ccaccgaaag gaccacgtag
     5761 atgatcaaca cacaaacccg acaccgcatc cttcggatcg ccacgctcgg cggcgtcgga
     5821 ctgaccgtcg tcgccagcgg cctggccatt cgcgcggtgc ggtccctcga cgaggccttc
     5881 accagcgcta tctcggaccc atatctggcg actgaggagt cctgagtcca cggggtcgtt
     5941 ggggcacgtg ccacgtgcac ggtcgcgtga cagtcggccg agccgatctg ctggggatgc
     6001 cgtgacctgt gttcgcgacg gcacagcctg gctctgtgtc caggcccaaa ccacggcgtc
     6061 ctgccacagc acgcggccac gccggagtcc gtggtcagcg tggcggttgg gaagctcctg
     6121 tggcgcaccc acgaccttgt gtggcccttc gcgagcttca agaggacgat gaggtctact
     6181 gtccagacga ctggagaccc gagaacatcg tcgtcaccgc cgatggctag ttgtggtcga
     6241 ccttgatctc gcgaaggcac agccccagaa cgcgcgccgt cgaccttgaa gtccacgact
     6301 tcggtaagcc cttcggccat aacccacgga tccgtgacga gttcatgacg gggtaccgtg
     6361 atcgttcctg aatcgggatc gagcgggagc gttgtcgctg gaccgcacag gcggtaacaa
     6421 cgcctgcacc atggactgtg aggcggcgtc ctgctcgcct gctacggctt ggtgttgtcg
     6481 atgggtccga tggcgtggga ccactgtcct gaccagctgt aagcgacccg tgaacgcgcc
     6541 accccgtcag tcaaggggag acccacaggc ccaggagtcg cccttgacgc aatcgtggag
     6601 caagccggca ggggttgcca cctgtcgcgc gaacgtgaag gtgccctgct cgtggggacc
     6661 cggtgtcctg ggcgcacccc ggttaggctt gcgtcatgtc cgtctcgctc ggtatgcc
//
LOCUS       DAOMZY010000191         6708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_10667_length_6708_cov_6.093041, whole genome shotgun sequence.
ACCESSION   DAOMZY010000191 DAOMZY010000000
VERSION     DAOMZY010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6708
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10667_length_6708_cov_6.093041"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1355
                     /locus_tag="PLK64_10975"
     CDS             <1..1355
                     /locus_tag="PLK64_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPV80314.1"
                     /translation="SPEAINLAEDRGAFGRVLAEANLPAPRHGTAYSAQEAVAVAHEI
                     GYPVLVRPSYVLGGRGMQIVYDDATLTTYVDAATIASPEHPILVDRFLDDAIEIDVDV
                     LYDGVDMYLGGVMEHIEEAGIHSGDSACTLPPATLGAAEIERVRASSLALAHGIGVRG
                     LMNVQFALAQDVLYVLEANPRASRTVPFVAKATGVPLAKAAARIMLGSSIAALREEGL
                     LPPTGDGGTLPAHAPMSVKEAVLPFNRFRTKEGQMVDALLGPEMKSTGEVMGIDTDFG
                     TAFAKSQLAAYGGLPTSGTVFVSVANRDKRAMIFPVRALAELGFTLLATSGTADVLRR
                     NGIKAEVVRKITERTGDGPVGEGDVIEATIADRIIAGDVAMVVNTPTGQTARADGYAI
                     RAAAIAADVTLITTVQELGAAVQGIESMRSAPMRVTSLQEHARALNLYGITNPDGVAR
                     "
     gene            1415..2248
                     /locus_tag="PLK64_10980"
     CDS             1415..2248
                     /locus_tag="PLK64_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HPV80315.1"
                     /translation="MRSSEPAGAYQHLVVAAPAIAALARPGQFVAATVGGATSGMLLR
                     RSFSIHAVDPDAGTVEVVVAAHAAGTAWITERRIDDVLDLVGPLGQAFPLPDGGGAAV
                     LVGGGYGSAPLFWLAQVLRQRGVHVEIVLGAASADRLFGVALAAEVADAVDVTTDDGS
                     AGTRGWVSDVLPEVLARSGATDVYACGPMAMLRAVTEAASAAGAAAHVAVEEAMACGV
                     GVCMTCVMPVIGADGRTRMVRACLDGPVFDGDRVRWDAFGDGLCRVPDDAVGAPQSGG
                     H"
     gene            2248..3207
                     /locus_tag="PLK64_10985"
     CDS             2248..3207
                     /locus_tag="PLK64_10985"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HPV80316.1"
                     /translation="MVDLSTTLAGATLPNPLMTASGCAANGKELHRFFDISRLGAFVT
                     KTVMPAARSGRGTPRMAETPSGMLNSIGLQGPGIDAFADEDLPWLASVGARVLASIAG
                     GTPDEFAQVATRLLSSPAAASVSGIEVNISCPNVASRGLVFAIDPLASAAVIAAVRAA
                     VPAHLPVFAKLSPDVTDLVTIASACLGAGADGLTLINTTLGMAIDLDRMRPHLGGITG
                     GLSGPAIRPIAVRAIWQVRAAMFAERIPTAPIIGVGGVASGRDALELAAAGASAIQVG
                     TATFNDPEAPVRVLDELAGLLDERGYAAFADVVAAAHPERRRQ"
     gene            3204..4091
                     /gene="pyrF"
                     /locus_tag="PLK64_10990"
     CDS             3204..4091
                     /gene="pyrF"
                     /locus_tag="PLK64_10990"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPV80317.1"
                     /translation="MTTSAQASSLRHPPSFGVRLHQAMRAHGPLCAGVDPHRGLMQAW
                     GLDYTVAGLERFAMTCAEAFAGRVAAVKPQSAFFEVFGSAGVAVLERLVADLRAAGTL
                     VILDVKRGDIGTTMDAYAEAFLGKDAPEPPDAITLSPYLGYGSLRPAIDLAHATGRGV
                     FVLALTSNPEGADVQHAVLHGQSVAGHVVAGATADNADAVARGELGSVGLVVGATVGD
                     AVDRLGLDLAGMGGPFLAPGVGAQGGTVESLRRVFGTALPHVLAASSREVLSAGPSVA
                     ALATRAQDTAAALAEALAS"
     gene            4247..4564
                     /gene="mihF"
                     /locus_tag="PLK64_10995"
     CDS             4247..4564
                     /gene="mihF"
                     /locus_tag="PLK64_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HPV80318.1"
                     /translation="MALPELTPQERAAALEKAALARRARAAVKNRLKYSQGSLAEVIT
                     EGKDDPVLGKLKVSALLESLPGVGPVRARQIMAEVGISESRRVRGLGVNQSAALIARF
                     DQS"
     gene            4561..5172
                     /gene="gmk"
                     /locus_tag="PLK64_11000"
     CDS             4561..5172
                     /gene="gmk"
                     /locus_tag="PLK64_11000"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPV80319.1"
                     /translation="MSGQDTLAEPFILPEGGAGRLVVLAGPTAVGKGTIAAYIRTHYP
                     QVWLSVSMTTRAPRPGEVDGVHYIFVADEEFDRLVAAGEFLEYAVVHGRAKYGTPRGP
                     VERALAAGRPALLEIDLQGARQVRDNLPDALLVFLAPPSWDELVNRLVGRGTETEEER
                     TVRLSTATRELAAAEEFDVVIVNDDVRRASEQLVSLMGSRPSA"
     gene            5221..5484
                     /gene="rpoZ"
                     /locus_tag="PLK64_11005"
     CDS             5221..5484
                     /gene="rpoZ"
                     /locus_tag="PLK64_11005"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPV80320.1"
                     /translation="MSGTVANPIGITNPPIDDLLAIADSKYALVIYSAKRARQINAYY
                     SQLQEGLLDYVGPLVETEVHEKPLSIALREINEGLLTAQATEG"
     gene            5533..>6708
                     /gene="coaBC"
                     /locus_tag="PLK64_11010"
     CDS             5533..>6708
                     /gene="coaBC"
                     /locus_tag="PLK64_11010"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HPV80321.1"
                     /translation="MRVVLGVSGGIAAYKAASLLRRFTESGHDVTVVPTEAALQFVGA
                     PTWAALSGHPVATDVWTAVDRVPHVRIGQDADLVVVAPATADLLAKAATGQAGDLLTA
                     TLLTARCPVVFAPAMHTEMWLHPATQANVATLRSRGAHVIEPSSGRLTGADTGPGRLP
                     EPEDLYAACLAVVAAADPAVRAEAAGDPRGDVRGDLRGVRVLVTAGGTREALDPVRYL
                     GNRSSGKQGVALAQVAAARGAEVTLVACNLSAAVGGSGSGGAIRVVPVESALDLQAAV
                     TTACADVDVVVMCAAVADFRPKHYVETKIKKTHAADGSDDSAPVIELVRNPDILAGLV
                     VGRGAAARPIIVGFAAETGDETGSVVELARVKLARKGCDLLVANEVGATKTFGEDTNT
                     "
BASE COUNT          925 a         2374 c         2361 g         1048 t
ORIGIN      
        1 cctcgcccga ggccatcaac ctcgccgagg accgaggtgc cttcggccgg gtcctcgcgg
       61 aggcgaacct gcccgcgccc cggcacggga cggcatacag cgcgcaggag gcggtcgcgg
      121 tcgcgcacga gatcggttat ccggtgctcg tccgcccgtc ctacgtcctg ggtgggcgcg
      181 ggatgcagat cgtctacgac gacgcgaccc tcacgaccta cgtcgacgcg gcgaccatcg
      241 cttcacccga gcacccgatc ctcgtcgacc ggtttctcga tgatgccatc gagatcgacg
      301 tcgacgtgct ttacgacggc gtcgacatgt atctcggcgg ggtcatggag cacatcgagg
      361 aggcgggcat ccactccggt gacagtgcct gcaccctccc gccggccacc ctcggggcgg
      421 ccgagatcga gcgggtgcgc gcctcgtcgc tggccttggc ccacggcatc ggcgtgcggg
      481 ggttgatgaa cgtccagttc gcgctcgctc aggacgtcct ctacgtcctc gaggccaacc
      541 cacgagcttc tcggaccgtg ccgttcgtcg ccaaggcgac cggggtgccg ctggccaagg
      601 cggcggcgcg catcatgctc ggctcgagca tcgctgcgct gcgcgaggaa ggcttgctgc
      661 cgcccacggg cgacggcggg accttgccgg cccacgcgcc gatgtcggtc aaggaagccg
      721 ttctgccgtt caaccggttc cggaccaagg aagggcagat ggtcgatgcc ctcctcggcc
      781 cggagatgaa gtcgaccggc gaggtcatgg gcatcgacac cgacttcggg acggccttcg
      841 ccaagagcca actcgccgcc tacggcgggc tgcccacgag tgggaccgtc ttcgtgtcgg
      901 tcgccaaccg tgacaagcgg gcgatgatct tcccggtccg ggcgttggcc gagctgggct
      961 tcaccctgct tgccacctcg gggaccgcag atgtgttgcg ccgcaacgga atcaaggctg
     1021 aggtcgtccg caagatcacc gagcggacgg gtgatggccc agtcggcgag ggggacgtga
     1081 tcgaggcgac catcgccgac cggatcatcg ccggagatgt tgcgatggtc gtcaacaccc
     1141 ccaccgggca gacggcccgc gcggacggct acgccatccg ggcggccgcc atcgcggccg
     1201 acgtcacgct catcacgacg gtccaagagc ttggcgctgc ggttcagggc atcgagtcga
     1261 tgcgctcggc acccatgcgg gtcacgtcct tgcaggagca cgcccgggcc ctgaacctct
     1321 acggcatcac gaaccccgat ggcgtggcgc ggtgagcggc gcggcatacc ccgtcggcgt
     1381 ctcagcccgc gacgtcgcgc acgagcgcgc cccggtgcgc tccagtgagc ccgccggtgc
     1441 ctaccagcac ctcgtcgtcg cggccccggc catcgccgcg ctggcccgcc cgggacagtt
     1501 cgtggcagcc accgtcggcg gggcgacatc ggggatgctg ctgcgtcgct ccttctcgat
     1561 ccacgccgtc gaccccgacg ccgggaccgt cgaggtcgtc gtcgcggccc acgccgcggg
     1621 gacggcgtgg atcaccgagc gccggatcga cgatgtcctc gatctcgtcg gaccgctcgg
     1681 gcaagcgttt cccctgcctg acggcggggg tgctgccgtg ctcgtcggcg gtggttacgg
     1741 aagcgcgccg ctcttctggc tggcccaggt cctgcgccag cgcggcgtcc acgtcgagat
     1801 cgtccttgga gcagccagcg ccgaccggct gttcggcgtc gccctggccg ccgaggtggc
     1861 cgatgccgtc gacgtgacga ccgacgacgg gtccgccgga acgcgcggct gggtgagcga
     1921 cgtgcttccc gaggtgttgg cccgctccgg tgccacagac gtctatgcct gcggtccgat
     1981 ggcgatgttg cgtgctgtga ccgaggctgc gagcgcggca ggcgcggctg cccacgtcgc
     2041 cgtcgaagag gcgatggcct gtggcgtcgg cgtctgcatg acctgcgtca tgccggtgat
     2101 cggagccgac ggccgcaccc gcatggtgcg tgcttgcctg gacggtccgg tcttcgatgg
     2161 cgatcgggtc cggtgggacg ccttcggcga cgggttgtgc cgggtgccgg acgacgccgt
     2221 cggagcgccg cagtcaggag ggcactgatg gtcgatctca gcacgacgct ggccggagcc
     2281 acgctgccca acccgctgat gacggcgtcc ggttgcgcgg ccaacggcaa ggaactgcac
     2341 cggttcttcg acatctcgcg cttgggcgcc ttcgtcacca agaccgtcat gcccgctgcc
     2401 cgcagcggcc gtggaacgcc ccggatggcc gagacgccgt cgggaatgct caactcgatt
     2461 ggcctccagg gtcctggcat cgacgccttc gccgacgagg acctcccgtg gctggcctcg
     2521 gttggcgccc gcgtgttggc ctcgatcgcg ggtggcacgc ctgacgaatt cgcccaggtg
     2581 gcaacgagac tgctctccag tccggccgcc gcgtcggtgt cgggcatcga ggtcaacatc
     2641 tcgtgtccca acgtcgccag ccgcgggttg gtcttcgcca tcgacccgct cgcgtccgct
     2701 gcggtcatcg ccgcagtgcg tgccgccgtc cctgcgcacc tgccggtgtt cgcgaagctc
     2761 agccccgacg tcaccgacct cgtgaccatc gcctcggcgt gcctgggcgc gggtgccgac
     2821 ggcctcacgc tcatcaacac gacgctgggg atggccatcg atctcgatcg gatgcgcccg
     2881 cacctcgggg ggatcaccgg cggcctgagc ggcccggcga tccggcccat cgcggtccga
     2941 gccatctggc aggtccgcgc cgcgatgttc gccgagcgga tccccaccgc accgatcatc
     3001 ggcgtcgggg gagtggcgtc cgggcgcgac gcgctcgagt tggctgccgc gggtgccagt
     3061 gcgatccagg tgggcacggc caccttcaac gaccccgagg ccccggtccg cgtcctagat
     3121 gagcttgcag gactgctcga cgagcgtggg tatgccgcgt tcgccgatgt cgttgcggca
     3181 gcccaccccg aacggagacg ccaatgacca catctgcgca ggcctcgtcg ctgcgccatc
     3241 cgccgtcgtt cggcgtgcgc ctgcaccagg cgatgcgggc ccatgggccg ctatgcgccg
     3301 gcgtggatcc gcaccgcggg ctgatgcagg cctggggatt ggactacacg gtggccgggt
     3361 tggagcggtt cgcgatgacc tgcgccgaag ccttcgccgg gcgggtggcc gcggtcaaac
     3421 cgcaatcggc gttcttcgag gtcttcggct cggccggggt cgccgtgctc gagcgactcg
     3481 tggccgacct gcgggctgcc ggcacgttgg tgatcctcga cgtcaagcgg ggggacatcg
     3541 ggacgaccat ggatgcctat gccgaggcgt tcctcggcaa ggacgcgccc gagcctcctg
     3601 acgccatcac cctgagcccc tacctcggat atggctccct gcggccggcg atcgacctcg
     3661 cccacgcgac cgggcgcggt gtctttgtcc tcgcgctcac gtcaaacccg gagggcgcag
     3721 acgtccagca cgctgtcctg catgggcaga gcgtcgcggg gcatgtcgtg gcgggagcca
     3781 ccgcggacaa cgcggacgcg gtggcccggg gggagctcgg ctcggtcggg ctcgtggtcg
     3841 gggcgaccgt cggggacgcc gtcgaccggt tgggtctgga cctggccggg atgggcggtc
     3901 cctttctagc tcccggggtc ggggcgcaag gaggcaccgt cgagagcctg cgccgggtct
     3961 tcggcacggc gctcccgcat gtcctggccg cgtccagccg ggaggtcctc agcgccggcc
     4021 ccagcgtcgc cgcgctggcc acccgggcgc aggacaccgc tgcggccctg gccgaggctc
     4081 tggcgtcgtg acctgcggtg ttgccgccgg gggaccgccc tgtgatcagg cagggggctg
     4141 cgcaggctca gggaggcgtg aactttcgcc tgtcgttgcg acgggccgga cgcgtcggta
     4201 ggttcggtgt ttgatgtcgt cccagagcac gatcgaggag tgaccggtgg ccctgcctga
     4261 gctgaccccg caagaacgag cggctgctct cgagaaggct gccctcgccc gccgggcccg
     4321 ggccgccgtc aagaaccgcc tgaagtactc ccagggctcc ctcgccgagg tgatcaccga
     4381 aggcaaggac gacccggtgc tgggcaaact caaggtgtcg gcgctgctcg agtcattgcc
     4441 gggggtcggc ccggtccggg cccgccagat catggccgag gtcgggatct ccgagtctcg
     4501 ccgggtccgc gggctggggg tcaaccagtc ggcggccctc atcgcccggt tcgatcagtc
     4561 gtgagcgggc aggacaccct cgccgaaccg ttcatcctcc ccgaaggagg tgccggacgg
     4621 ttggtcgttc tcgccggccc gaccgcagtc gggaagggga cgatcgcggc atacatccgc
     4681 acccactatc cccaggtgtg gctgtcggtc tcgatgacga cgcgcgcgcc gcgtcccggt
     4741 gaggttgacg gcgtccacta catcttcgtc gccgacgagg agttcgaccg gctggtcgcg
     4801 gcaggggagt tcctcgagta tgccgtcgtc cacggtcgcg cgaagtacgg caccccccgt
     4861 ggtcccgtcg agcgcgccct ggccgccggg cgtccggccc tgttggagat cgacctgcag
     4921 ggtgcccggc aggtccgcga caaccttcct gacgcgctgc tggtcttcct cgcccctccg
     4981 agttgggacg agttggtcaa ccggttggtc ggccggggga ccgagaccga ggaggagcgg
     5041 actgtccggt tgtcgaccgc cacccgcgaa ttggcggcgg ccgaagagtt cgatgtcgtc
     5101 atcgtcaacg acgatgtccg ccgggccagt gaacaactcg tatcattgat gggatcgcgt
     5161 ccttctgcgt gaaggcgccc ctagcgaccg caagcccccg atcgaaacga gatcctcgcc
     5221 gtgtccggaa ccgtggccaa ccccatcggc atcaccaacc cgcccatcga cgacctgctc
     5281 gccatcgccg acagcaagta cgcgctggtc atctactccg ccaagcgggc ccggcagatc
     5341 aacgcctact actcgcagtt gcaggagggt ctgctcgact acgtcggccc cctcgttgag
     5401 accgaggtcc acgagaagcc gctgtcgatc gcgctgcgtg agatcaacga aggcctgctc
     5461 accgcgcagg ccaccgaggg ctgagccagc agaccccgtc gggcgacgtc gaccgatcgg
     5521 agccagagca tcatgcgcgt cgtcctgggc gtgagcggcg gcatcgccgc ctacaaggcc
     5581 gcttccctgt tgcgtcggtt caccgagagc ggtcatgacg tcaccgtcgt ccccaccgag
     5641 gctgcgttgc agttcgtcgg tgcgcccacg tgggcggcgc tctcgggaca tccggtcgcc
     5701 accgacgtgt ggacggccgt cgaccgggtc ccgcatgtgc gcatcggcca ggatgccgac
     5761 cttgtcgtcg tcgccccggc gaccgccgac ctgttggcca aggcggcgac cggtcaggcc
     5821 ggtgacctcc tcaccgccac cctgctcacc gcacgctgcc cggtcgtctt cgcgccggcg
     5881 atgcataccg agatgtggct tcacccggcg acccaggcca acgtcgccac cctgcgctcg
     5941 cgcggcgcgc acgtcatcga gccttcgtcc ggccggctca cgggggccga caccggtcct
     6001 gggaggcttc cggagcccga ggacctgtat gccgcgtgcc tggccgtcgt cgcagccgcc
     6061 gacccggcag tgcgtgccga ggcagcgggc gacccgcgcg gcgacgtgcg cggcgacctg
     6121 cgcggcgtcc gcgtcctcgt caccgcgggg ggcacccgtg aggccctgga tcccgtgcgc
     6181 tacctgggca atcggtcctc cggcaaacaa ggggtggcct tggcccaggt ggccgcggcg
     6241 cgtggcgccg aggtgacgct cgtggcgtgc aacctcagcg cggcggtcgg cggttcgggc
     6301 tccggcggtg cgatccgggt cgtgccggtc gagtctgccc tcgacctgca agcggccgtg
     6361 accactgcct gcgcggatgt cgacgtcgtc gtcatgtgtg cggcagtggc ggacttccgg
     6421 cccaagcact atgtcgagac caagatcaag aagacccatg cggccgacgg atcggacgac
     6481 tcggccccgg tgatcgagtt ggtccgcaat cccgacatcc tcgccggcct cgtggtcggg
     6541 cggggcgccg ccgcccggcc gatcatcgtc ggcttcgcag ccgagaccgg cgacgaaacc
     6601 ggatcggtcg tggagttggc ccgggtcaag ctcgctcgca aggggtgtga cctgctcgtg
     6661 gccaacgagg tcggcgccac caagaccttc ggcgaagaca ccaacacg
//
LOCUS       DAOMZY010000192         6690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_10709_length_6690_cov_6.630294, whole genome shotgun sequence.
ACCESSION   DAOMZY010000192 DAOMZY010000000
VERSION     DAOMZY010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6690
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10709_length_6690_cov_6.630294"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..676)
                     /locus_tag="PLK64_11015"
     CDS             complement(152..676)
                     /locus_tag="PLK64_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPV80322.1"
                     /translation="MNGSGVDLVDPVDPVDPGRFRTAFGRFATGVSVVTCLVDGSDHA
                     MTANSLTSVSLAPPLVLVCVERETRFHEAILTAGHWGVSVLGAQARGAAAWFATRGRP
                     LAGQLDPVAHHRSSLTGVALLDDALAWFEVRTTAVYDGGDHSIVVGEVVALSLAEEPP
                     AALTFFRGQYGHLD"
     gene            complement(673..1545)
                     /gene="mshB"
                     /locus_tag="PLK64_11020"
     CDS             complement(673..1545)
                     /gene="mshB"
                     /locus_tag="PLK64_11020"
                     /EC_number="3.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-
                     D-glucopyranoside deacetylase"
                     /protein_id="HPV80323.1"
                     /translation="MARLLFVHAHPDDETLATGVAIAHYVSRGHDVEVLTATLGEEGE
                     VIPADVRHRTSDRDDTLGEFRAGELAGAMARLGARHTFLGDDGSGPRWRDSGMAGMPS
                     ADHPRAFAGADVAEAARLVAAYLRERRPDVVVTYDVDGGYGHPDHIQTRRVVQAAITG
                     LAPAERPGRFFETLTPRSWVVTDRQWLAEQVPEASGLHIPTQDEPYAVSVVEDAIVSH
                     VVRDAVAVERQASALAAHRTQVSVFGGYYSLSNDIAARLSGREAFARLDPATGERLPR
                     TSDTWFEGLLVDLP"
     gene            complement(1545..2582)
                     /locus_tag="PLK64_11025"
     CDS             complement(1545..2582)
                     /locus_tag="PLK64_11025"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HPV80324.1"
                     /translation="MTIQTSTGAAPSATPAGRPLLRLTNLTKHFPQFRQTLIKRPDNP
                     VRAVDGISFDVAPGQTVGLVGESGCGKSTAGRTLLRLLEPTAGTIEFEGRDITHAKGA
                     ELRTLRREMQMIFQDPYGSLNPRQTIGSIIAAPYDIQKITPEGGRRAAVQGLMERVGL
                     NPEHYNRYPHEFSGGQRQRIGVARAIALKPKLIVCDEPVSALDVSIQAQVVNLLEDIQ
                     EEQGLAYVFIAHDLSVVRHISDRVIVMYLGKIMEEADRDELYEAPLHPYTHALLSAVP
                     VPDPKRIEHKERILLKGDLPSPQNPPSGCVFRTRCPKAQERCTVEVPLPVELSPGHRV
                     ACHFPEARTVL"
     gene            complement(2593..3678)
                     /locus_tag="PLK64_11030"
     CDS             complement(2593..3678)
                     /locus_tag="PLK64_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV80325.1"
                     /translation="MADTSGATVTTSPSARAAGERTAYLDVRDLKVHFPTDDGLVKAV
                     DGLNFTVERGKTIGIVGESGSGKSVTSQGILGLHRRTRARASGQIWLDGEDLMTVTDE
                     RLRELRGNKMAMIFQDPLSALHPYYTIGSQIVEAIRVHHSDTSKSAAKAKAVDLLGLV
                     GIPNPARRVDQYPHEFSGGMRQRAMIAMALANDPSLLIADEPTTALDVTVQAQILDLM
                     RKLQAEFGSAIIMITHDLGVVAEMADDILVMYGGKAVEHGPVNDVFYEPEHPYTWGLL
                     TSMPRLDRARLSRLNPVPGNPPSLINVPSGCAFHPRCAFTGQVPADACRAAVPDLTAA
                     GPGHLTRCHLEPATRAELFASEIKPRL"
     gene            complement(3681..4694)
                     /locus_tag="PLK64_11035"
     CDS             complement(3681..4694)
                     /locus_tag="PLK64_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV80326.1"
                     /translation="MFFYIIRRLISVVVMLFIISMTTFLVFFASPVDPASLTCAKNCT
                     PAVIEGNRKLLGFDKPITVQYANFVKGLFVARDFPDDPEQKARAPQTVFTCAAPCLGY
                     SQRQAKSVTEIIKDGLPITASIALGAFVLWITSGVFFGIIAALNRGRWIDRLLVGVSL
                     IGYSLPSFFIGQLLLVFIAIKWQLWPIPNFVPLSEGLWPWMQSIFLPSLTLAAIFGAS
                     YVRLTRAYMLETMSEDYIRTARAKGVKESVVVRRHGLRAALTPIVTAAGLDLGGLLGG
                     AIISEQVFSFRGLGWTSIQAITNTDLPVIVAVVVVASFFIVIANLVVDVLYGVIDPRV
                     RMS"
     gene            complement(4833..6560)
                     /locus_tag="PLK64_11040"
     CDS             complement(4833..6560)
                     /locus_tag="PLK64_11040"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPV80327.1"
                     /translation="MMRMKKGAAAVALGAAVAMTAAACSSGSTPSTTTASTAAAVAKG
                     GTLYILNLGPHNGLDVQQSYVGADLQTANRVYMRYLTSYSTGANPKLVPDLATDLGTM
                     SDGGKTWKFTIVDNAKWQDGAKVTCEDVKYGGSRTFATDLISNGPTYSLQFLDIPNDA
                     DGTSSYKGPYNGTGQALYDKAVTCDGQTLTLHFKTPWADFNQATAALGIFAPFPKAQD
                     KADKSLFAPWSNGPYMLEGTFDPAKGGTWVRNPNWDAATDPIRKAYPDKIVDVEGIQG
                     ETVYQRLLADGGDDKTAITFGTAPPSVLPTIASTPGADKRMANVPAPYVDYIQPNMTS
                     TAMSNPKVRQAFAMATNRDAYITANGGPQVMVPTYAMCNKVLACYKDFNPFGAPTAGD
                     PDGAKKVLTDAGVAMPVNITVVYRKTPTRDKSFAALKETWDKAGFNVTLEGITDKYYR
                     TISGAAMKTKDAFYASWGADWPSGSTDIPPLFDGRINITPTSSNQDYGYYNSDAANKA
                     MDAAYLITDPAAREKAWGDIDEMISKDGGVIPLVNQKFTFVYGSGVKNFEVNTLFGGY
                     ADLANIAVK"
BASE COUNT         1076 a         2118 c         2377 g         1119 t
ORIGIN      
        1 cgtacgcccc cacgaggatc gcgacggcca ccacggaccg gacgacgatc cgactccact
       61 cacgcacgcc gactctcctt gccacgttga cccccgctac gtccgcacac ggtagcccac
      121 gaacctgaca ccccgttgaa tcgatcagca gtcagtcgag atgtccgtac tgcccacgga
      181 agaaggtcag cgctgccgga ggctcttcgg ccaggctgag cgcgaccacc tcgccgacga
      241 cgatgctgtg atcgccgccg tcgtagaccg cagtcgtgcg cacctcgaac cacgccaggg
      301 catcgtcgag caacgccacg ccggtcagcg aactgcgatg gtgcgcaacg ggatccaact
      361 gcccggccag cggccgacca cgcgtggcaa accaggcggc agcgccccgc gcctgggcgc
      421 cgagcacgct cacgccccag tgcccagccg tgaggatcgc ctcgtggaac cgcgtctcgc
      481 gctcgacgca aacgaggacg aggggcgggg ccagagagac cgaggtcagg gagtttgccg
      541 tcatcgcatg gtcggagccg tcgacgagac aggtgacgac gctcactccg gtggcaaagc
      601 gcccgaatgc ggtccggaac cgccccggat caaccggatc aaccggatcg accagatcga
      661 cgccgcttcc gttcacggca ggtccaccag cagaccctcg aaccaggtgt ccgacgtccg
      721 aggcagccgt tcaccggtcg ccggatccag gcgcgcgaac gcctctcggc cggacaaccg
      781 ggcggcgatg tcgttggaga ggctgtagta gccgccgaac acgctcacct gggtccggtg
      841 ggcggccagg gccgaggcct gccgctcgac tgcgacggcg tcgcggacga catggctgac
      901 gatggcgtcc tcgacgacgg acacggcata cggctcgtcc tgggtgggga tgtgcagtcc
      961 gctcgcctcg ggcacctgct cggcgagcca ctgccgatcg gtgaccaccc acgaccgcgg
     1021 tgtcagggtc tcgaagaacc gtcccggccg ctcggccggg gccagcccgg tgatcgcggc
     1081 ctgcacgacc cgccgggtct ggatgtggtc cgggtggccg tagccaccgt ccacgtcgta
     1141 cgtcaccacg acgtccggac gccgttcgcg caggtatgcc gccaccaggc gtgcggcctc
     1201 ggccacgtcg gccccggcga aggcgcgcgg gtggtcggcg gacggcatac cggccatgcc
     1261 cgagtcgcgc caccgcggac cggacccgtc atctccgagg aaggtatgcc gcgcacccag
     1321 gcgagccatc gcgccggcca actcgcccgc gcggaactct ccgagggtgt cgtcccggtc
     1381 ggacgtgcga tgccgcacat cggccgggat cacctcgccc tcctcgccga gggtcgcggt
     1441 gaggacctcg acatcgtggc cgcgcgagac gtagtgagcg atggcgacgc cggtggcaag
     1501 ggtctcgtcg tcgggatggg cgtggacgaa gagcagacgg gccattacag aacagtacgt
     1561 gcctcgggga agtgacaggc cactcggtga cccggactga gctccacggg caggggaacc
     1621 tcgacggtgc agcgctcctg ggccttcggg caacgggtgc ggaagacgca cccgctcggc
     1681 ggattctgcg ggctgggcag gtcgcccttg agcaggatcc gctccttgtg ctcgatccgc
     1741 ttggggtcgg gcaccggcac ggccgagagc agggcatggg tgtaggggtg caggggcgcc
     1801 tcgtagagct cgtcccggtc ggcctcttcc atgatcttgc ccaggtacat gacgatgacc
     1861 cggtcggaga tgtggcggac caccgagagg tcgtgcgcga tgaagacgta ggccagaccc
     1921 tgctcctcct ggatgtcctc cagaaggttg acgacctgtg cctggatcga cacgtcgagg
     1981 gccgacacgg gttcgtcgca gacgatgagc ttaggcttga gcgcgatcgc ccgggccacc
     2041 ccgatgcgct ggcgctgccc gccggagaac tcgtgcgggt aacggttgta gtgctcgggg
     2101 ttgagcccga cccgctccat gaggccctgg acggccgccc gacgaccacc ctcgggggtg
     2161 atcttctgga tgtcgtaggg cgccgcgatg atcgagccga tggtctggcg cgggttgagc
     2221 gagccgtagg ggtcctggaa gatcatctgc atctcgcgcc gcagcgtgcg caactcggca
     2281 cccttggcgt gggtgatgtc gcggccctcg aactcgatgg ttccggccgt cggctcaagc
     2341 agccgcagca gggtccgccc cgcggtcgac ttgccacagc cggactcccc caccaggccg
     2401 acggtctgcc ccggcgcgac gtcgaacgag atcccgtcga cggcacgcac cgggttgtcc
     2461 ggtcgcttga tgagggtctg gcggaactgc gggaagtgct tggtgagatt ggtcaaccgc
     2521 aacagtggcc gacccgccgg ggtggccgac ggtgcggcgc cggtgctcgt ctgaatggtc
     2581 atgcgcccct cctcacagcc tgggcttgat ctcgctggcg aacagctcgg cgcgcgtggc
     2641 cggctccaga tggcagcggg tcaggtgacc cggaccggct gccgtcaggt cggggaccgc
     2701 cgctcgacac gcgtcggcgg gcacctgacc ggtgaaggcg cagcgcgggt ggaaggcgca
     2761 gcccgacggc acgttgatga gcgagggcgg gttccccgga acgggattga ggcgactgag
     2821 ccgggcccgg tccaaccgag gcatcgacgt cagcagaccc cacgtgtagg ggtgctcggg
     2881 ctcgtaaaac acgtcgttga ccgggccgtg ttccacggct ttgccgccat acatgacgag
     2941 gatgtcgtcg gccatctcgg cgaccacgcc gaggtcgtgg gtgatcatga tgatcgccga
     3001 gccgaactca gcctgcagtt tgcgcatgag gtcaaggatc tgggcctgca ccgtcacgtc
     3061 gagcgccgtc gtcggttcgt cggcgatgag caggctgggg tcgttggcca gagccatcgc
     3121 gatcatggcc cgctgacgca tcccaccgga aaactcgtgg gggtattggt cgacccgtcg
     3181 ggccgggttg gggatgccga ccagaccgag caggtcgacc gctttggcct tggcagcgga
     3241 cttcgaggtg tcggagtggt gcacccggat cgcctcgacg atctgcgacc cgatcgtgta
     3301 gtaggggtgc agcgccgaga gcgggtcttg gaagatcatc gccatcttgt tgccgcgcag
     3361 ctcgcgcagc cgctcgtcgg tgaccgtcat gaggtcctcg ccgtcgagcc agatctggcc
     3421 ggaggcccgc gctcgggtgc gccggtgcag gccgaggatc ccttggctgg tcaccgactt
     3481 gcccgagccg gactcaccga cgatgccgat ggtcttgccc cgctcgaccg tgaagttcag
     3541 cccgtcgacg gccttgacca gaccgtcatc ggtcgggaag tgcaccttga ggtcgcgcac
     3601 atcgaggtat gccgtgcgct caccggccgc tctagcggac ggcgacgtgg tgacggtggc
     3661 tccagaggtg tcggccatgg tcagctcatc ctcacgcgcg ggtcgatgac gccgtagagg
     3721 acgtcgacga cgaggttggc gatgactatg aagaacgagg ccacgacgac caccgcaacg
     3781 atgacgggaa ggtcggtgtt ggtgatggcc tggatcgacg tccagcccag tccgcggaag
     3841 ctgaagacct gctcgctgat gatggccccg ccgagcagac cgcccaggtc gagaccggca
     3901 gcggtgacga tcggggtgag cgctgcgcgc agcccgtggc gccggacgac gaccgactcc
     3961 ttgacgccct tggctcgggc ggttcggatg tagtcctccg acatcgtctc aagcatgtag
     4021 gcgcgggtga ggcggacgta ggacgccccg aagatggcgg cgagggtgag tgagggcagg
     4081 aagatgctct gcatccacgg ccagagtccc tcggagagtg ggacgaagtt ggggatgggc
     4141 cagagctgcc acttgatggc gatgaagacg agcagcagtt ggccgatgaa gaagctcggc
     4201 agggagtagc cgatgaggga cacgccgacg agcagtcggt cgatccagcg ccctcgattg
     4261 agggccgcga tgatcccgaa gaagacaccg gacgtgatcc acaggacgaa ggcacccagg
     4321 gcgatggagg ccgtgatcgg cagaccgtcc ttgatgatct cggtcaccga cttggcctgg
     4381 cgctgcgagt aaccgaggca cggagcggcg caggtgaaca cggtctgcgg ggcgcgggcc
     4441 ttctgctcgg ggtcgtcggg gaagtcccgg gcgacgaaca ggcccttgac gaagttggcg
     4501 tactgcacgg tgatcggctt gtcgaagccc aagagcttgc ggttgccctc gatgaccgcc
     4561 ggcgtgcagt tcttcgcgca ggtgaggctc gccggatcga cgggcgaggc gaagaacacg
     4621 aggaatgtgg tcatgctgat gatgaagagc atgacgacga ccgagataag ccgcctgatg
     4681 atgtagaaga acatgggtca gggcacctcg cgggtgcgct caccttcctc tcggtccggg
     4741 gggcgactgt gccggacggg ggatgtcccc atccggcaca gtcggtagtc ctgcgacatc
     4801 ggtgccagcc gtgtcagcac cgacgtctgg gtttacttaa ccgcgatgtt cgccaggtcg
     4861 gcgtagccgc cgaacagggt gttgacctcg aagttcttga cgcccgaacc gtagacgaag
     4921 gtgaacttct ggttgaccag cgggatgacg ccgccgtcct tggagatcat ctcgtcgatg
     4981 tcgccccacg ccttctcacg ggcggccggg tcggtgatca ggtatgccgc gtccatggcc
     5041 ttgttggccg cgtcgctgtt gtagtagccg tagtcctggt tgctgctcgt cggcgtgatg
     5101 ttgatccgac catcgaacag cggcgggatg tccgtcgagc ccgacggcca gtcagcgccc
     5161 cagctggcgt agaaggcatc cttggtcttc atggccgccc cggagatggt gcggtagtac
     5221 ttgtcggtga tcccctcgag ggtcacgttg aagcctgcct tgtcccacgt ctccttcagc
     5281 gccgcgaacg acttgtcgcg ggtgggcgtc ttgcggtaga cgacggtgat gttgaccggc
     5341 atcgcgacac cagcgtcggt gagaaccttc ttcgcgccgt cggggtcacc ggcggtcgga
     5401 gcaccgaagg ggttgaagtc cttgtagcac gcgagcacct tgttgcacat cgcgtaggtg
     5461 gggaccatga cctgcggccc accgttggcg gtgatgtagg cgtcccggtt ggtcgccatg
     5521 gcgaaggcct ggcggacctt ggggttggac atcgccgtgc tcgtcatgtt cggctggatg
     5581 tagtcgacgt acggagccgg aacgttggcc atgcgcttgt cggcgcccgg cgtgctggcg
     5641 atcgtcggca gcaccgacgg cggagccgta ccgaaggtga tggcggtctt gtcgtcgcca
     5701 ccgtcggcca gcaggcgctg gtaaaccgtc tcgccctgga tgccctcgac gtcaacgatc
     5761 ttgtcggggt acgccttgcg gatcgggtcg gtcgccgcgt cccagttggg gttgcggacc
     5821 caggtgccac ccttggccgg gtcgaaggtg ccttccagca tgtacgggcc gttggaccag
     5881 ggggcgaaca ggctcttgtc agccttgtcc tgcgccttgg ggaacggagc gaagatgccc
     5941 agcgccgccg tggcctggtt gaagtcggcc cacggggtct tgaagtgcag cgtcagcgtc
     6001 tgaccgtcac aggtgacggc tttgtcatag agcgcctggc cggtgccgtt gtacgggccc
     6061 ttgtaggacg acgtgccgtc ggcgtcgttc gggatgtcga ggaactgcag cgagtaggtc
     6121 ggcccgttgg agatcaggtc ggtggcgaag gtacgcgagc cgccgtactt aacgtcctca
     6181 caggtgacct tggcgccgtc ctgccacttg gcgttgtcga cgatcgtgaa cttccacgtc
     6241 ttgccgccgt cgctcatcgt gccgaggtcg gtggcgaggt caggcaccag cttggggttc
     6301 gctcccgtcg agtagctggt gaggtagcgc atgtagaccc ggttggcggt ctgcaggtcc
     6361 gcgccgacgt aggactgctg aacgtccagg ccattgtgcg ggccgaggtt caggatgtag
     6421 agcgtcccgc ccttcgcgac tgccgcagcc gtcgacgcgg ttgtggtgct gggcgtcgag
     6481 cccgagctgc aggctgcagc tgtcattgcc accgccgcgc cgagggcgac ggcggccgcg
     6541 cccttcttca tgcgcatcat gcaatctcct tgtcgtactt ccgtcgcacg tgctacgacg
     6601 ggtcgtcggt ccgcgacggg cgtcgcgaac cgtcacttat cgctctcggg gggatagcga
     6661 taagtcgtgg gttctctgcg caccgcgcag
//
LOCUS       DAOMZY010000193         6678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_10738_length_6678_cov_6.505209, whole genome shotgun sequence.
ACCESSION   DAOMZY010000193 DAOMZY010000000
VERSION     DAOMZY010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6678
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10738_length_6678_cov_6.505209"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PLK64_11045"
     CDS             complement(<1..430)
                     /locus_tag="PLK64_11045"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPV80328.1"
                     /translation="MLAPASAVHTQRWANGLCSLGAEVHVACLAEHVPSVSTLDDRIT
                     VHTLSGRGQVAYFTQAREVAKLARRVRPDVVNAHYASGYGTLLRLAHLKPSVLSAWGS
                     DVYEFPDRGPLQRWLVAGNLRYAGAVTSSSNVMAARIAEVT"
     gene            complement(474..1235)
                     /locus_tag="PLK64_11050"
     CDS             complement(474..1235)
                     /locus_tag="PLK64_11050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPV80329.1"
                     /translation="MLGAEGALVSVVMPTYNCADFIEESIRSVQGQTHGEWELIVIDD
                     CSTDDTRERVERIAASDPRVHYHLLESNSGAAVARTLGMELARGRFIAFLDSDDLWRP
                     DKLALQLDFLNRTAGTIVCSAYDQIDEAGRSLGRVLSPKKRADYNAVLLTCPIGNSTV
                     LYDADALGKFVVPNIRKRNDDALWLQMLKKEKYILGMPDVLMSYRVRANSISANKLSL
                     IRYHWILYRDIEHLSIARSAAHIGVWVALKALRIK"
     gene            complement(1276..2442)
                     /locus_tag="PLK64_11055"
     CDS             complement(1276..2442)
                     /locus_tag="PLK64_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPV80330.1"
                     /translation="MKLTVAGLGYVGMANAVLLSQHHDVTAFDVDAQRVRLVNEGRSP
                     IADTEIEARLASGGLRLRATSDPVGAYSGAELVVVATPTNYDPETLYFDTSSVESVID
                     DVLAVAPRAIIVIKSTIPVGFTKRMQSLRPDARILFSPEFLREGHALYDNLHPSRIIV
                     ADPGPDAEVFAGLLVEGAEDADPPVMFTGSTEAEAIKLFSNTYLAMRVAYFNELDTYA
                     LQHGLDTRQIIRGVGLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYRDVPQVLIQAI
                     VESNTTRKDFIATDILASGPTTVGIYRLAMKSGSDNFRSSSVQGIMKRIKAKGVMVIV
                     YEPMLSEETFFNSEVVRDLAEFTARSDVIVCNRAAPELSDVRAKLYTRDVFGRD"
     gene            complement(2439..2912)
                     /locus_tag="PLK64_11060"
     CDS             complement(2439..2912)
                     /locus_tag="PLK64_11060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPV80331.1"
                     /translation="MAPRPAALPPNPWNEHAWVVGEPVVGEGTWIGAFTMIDASGGLT
                     IGAGCDISTGAQVYTHSSAKRCVSGRRFDVVERAPVVIGDRVFIGAGAIIQMGVTIGD
                     EAIIGAGAVVTKDVPAGAIVAGVPARVVGRVEFDSGGTPTLAYGRAMSIEGSDTP"
     gene            complement(2912..3847)
                     /locus_tag="PLK64_11065"
     CDS             complement(2912..3847)
                     /locus_tag="PLK64_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPV80332.1"
                     /translation="MSAETVFFTGGAGFIGLHVVPMLLERGYNVRIFDNMFRGDRSAV
                     EQLQAAHPGRIDLIDQDIRYGGAVHAAMAGCSVVIHAAAVSINKSQADPHESMAINTI
                     GSHNVIAAAADHGVRRLVFCSSASVYGDPKRLPMHEDDELSPQTPYCISKRTGEDLLD
                     FYQRRSGLSWIALRFFNVYGPGQKTTAYYTSVINHFVNRLKAGEPPVIDGKGEQSMDF
                     IHVHDIARSVVLALDCAGDNVPVNIGTGIDTTVAELARILIDAVGVDVQPQFSGRDVI
                     ASRRAADVTRAREVLGFEATIPVREGMTALIRDGG"
     gene            complement(3913..5058)
                     /locus_tag="PLK64_11070"
     CDS             complement(3913..5058)
                     /locus_tag="PLK64_11070"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPV80333.1"
                     /translation="MQRTVPLGQPTVGEPELAAVAEVFASGWLSGAGPTCVAFENEFA
                     RAVGTAHALTTANCGSALHLALQVLGAGPGDEVIVGDYTFPATGHAVVWTGATPVFAD
                     VRPDIWSADPAAVEAAITPRTVGILAVDVFGQPADYDELRAIADRHGLWIVEDAACAA
                     GATYHGRPAGSLADLAAFSFHGRKGITAGEGGALVGDRGDLMDQARKLHTYGIAPAVT
                     REGEATLPVPSFDEAGYNYRLSDVQAAIMRVQLGRLPDLLAARTRSAQVYAAGLGDLD
                     QLTLPVALDDRTHPWQSYVLTLDPSLDRGRVALALREQGVGCNFGTYASHVQPVYGTP
                     AGRECPVSADLFHRHLAIPMHANLTDDDLTHVVDTVRAVVGSDAVRR"
     gene            complement(5129..6310)
                     /locus_tag="PLK64_11075"
     CDS             complement(5129..6310)
                     /locus_tag="PLK64_11075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPV80334.1"
                     /translation="MRIVVVNNFFPPRVGGSSHLSDALARGYAARAHEVLVVTAAYGE
                     APAQEQRDGIRIVRFPAAMLPETRFAVSFDLSFASRPSLPRDLARLLDDFRPDVIHQH
                     GQFMDLTWATGAYARRRGIPVLLSVHTRLENPAALYRHAFRTLDATLVAPRLRRYRPR
                     IVVMDAHMRDYITARYRGGYRELVPIPVGVDPVWVRGGDAAVGRELAGVGDAPILLSV
                     GHVIPLRDRVGLVEALPAVLARHPDARLVVAGRVYYDVFLRRATELGVAYAVRPLGAV
                     PKASIPHLLAAATLESHEQGDGMGTATLEAMAAGVPIVGWGRLDNFPGVPLHDGGEVL
                     LTDRGDVVGLAQRLLRVLDDPVEAQAVGARGRALIDAHFTLDRVLDAHLATFSELLGR
                     A"
BASE COUNT         1185 a         2165 c         2227 g         1101 t
ORIGIN      
        1 gggtgacctc cgcgatccgg gcggccatca cgttgctgga ggacgtgacc gcgccggcgt
       61 agcgcaggtt gcctgcaacc aaccagcgct ggagtgggcc acggtcagga aactcgtaga
      121 cgtcgctccc ccacgcagac agaacggaag gcttgagatg ggcgagcctg agcaaggtgc
      181 catagccgct cgcgtaatgc gcattgacga catccggtcg cacccggcgc gccagtttcg
      241 cgacttcccg agcctgggtg aagtaggcaa cctggccgcg acctgacaat gtatggacgg
      301 tgattcggtc gtccaacgtg gacacgctcg gcacatgttc cgcaaggcag gctacgtgga
      361 cctccgcacc taggctgcac aggccgttgg cccaacgttg cgtgtgaact gcgctagccg
      421 gagccaacat aaggagccgc attcttagcg ccctaccccc gcctcgttgc ctatcacttg
      481 atcctcagcg ccttgagggc cacccacacg ccaatatgcg cggcagatcg ggcgatcgac
      541 aggtgctcga tgtcgcggta caggatccag tggtaccgga taagcgacag cttgttggcc
      601 gagatagagt tcgctcgcac ccggtagctc atcaaaacgt ccggcattcc gagaatgtat
      661 ttctctttct tcaacatctg aagccagagc gcgtcgtcat tccgcttccg aatgtttgga
      721 acgacaaact ttcccaacgc gtcggcgtcg tacaacacgg tggagttgcc aatggggcag
      781 gtcaagagga ccgcattgta gtccgcccgc ttcttgggcg agagcactcg cccaagggag
      841 cggccagctt catcgatctg atcgtaggca gagcaaacga tcgtccccgc ggttcgattg
      901 agaaagtcca gctggagggc tagtttgtcc ggtcgccaga ggtcgtcgct atcgagaaag
      961 gcgatgaatc gtccacgcgc aagttccatt cccagggttc tggccaccgc cgccccggaa
     1021 ttcgactcca agaggtggta gtggactcgc gggtccgatg cagcgatccg ttccacccgc
     1081 tcacgcgtgt cgtccgtgga gcaatcatcg atgacgatca actcccactc gccatgtgtt
     1141 tgaccctgaa ccgacctgat cgactcctcg atgaagtcgg cgcagttata cgttggcatc
     1201 acgacgctga cgagcgcccc ctcagcaccg agcatggttc agtcctccta ggatgggtgt
     1261 acctccccga catggtcagt ctcgaccgaa gacatccctg gtgtagagct tggcacgaac
     1321 gtcactgagc tcaggtgccg ctcgattgca gacgatcacg tctgagcgcg ccgtgaactc
     1381 agcaaggtcg cggacgactt cggagttgaa gaatgtttct tcgctgagca tcggttcgta
     1441 gacgatgacc ataaccccct tcgccttaat tctcttcatg atgccttgaa ctgacgacga
     1501 tcggaaattg tccgagcctg acttcatggc gaggcgatag atgcccacgg tggtaggccc
     1561 gctggccaag atgtccgtcg cgataaagtc cttacgagtc gtgttcgact cgacaattgc
     1621 ttgaatcaga acctgcggga cgtcccgata gttggcgagg agctgttttg tgtccttagg
     1681 aaggcagtaa ccaccgtatc cgaaactagg gttgttgtag tgggttccaa tgcgcgggtc
     1741 gagcccgacg ccgcgaatga tctgccgcgt gtccaggccg tgctgcagcg cgtaggtgtc
     1801 cagttcattg aagtaggcca cccgcatggc cagataggtg ttggagaaga gtttgatcgc
     1861 ctctgcctcc gtcgaccccg tgaacatgac tggcgggtcc gcatcctcgg cgccctcaac
     1921 gagcaggccc gcaaacactt cggcgtccgg ccctgggtcg gccacaatga tccggctggg
     1981 atgcaggttg tcatacaaag catgcccctc gcgcaggaac tctggcgaga agaggatgcg
     2041 ggcatccggc cgcagggatt gcatccgctt cgtgaagcct acgggaatcg tcgacttgat
     2101 gacgatgatt gccctcggtg caacagcaag gacgtcgtcg atgaccgatt cgacagaaga
     2161 tgtgtcgaag tagagcgtct cagggtcgta attcgtgggt gtggccacaa cgacgagctc
     2221 ggcgccggaa taggcaccga ccgggtcgct cgtcgcgcgc agcctcaatc cgccggaggc
     2281 caacctggcc tcgatctcgg tgtccgcgat tggagatcga ccctcgttca ctagtcgcac
     2341 gcgctgagcg tccacgtcga acgcagtgac gtcgtggtgc tgcgagagca gcacggcgtt
     2401 ggccatcccg acataaccga gcccggcaac cgtcaacttc atggcgtgtc cgacccttcg
     2461 atgctcatgg cacgaccata cgcaagggtt ggcgtgccgc cggaatcgaa ctcgacgcgg
     2521 cccaccaccc gagcgggaac tcccgcgacg atggccccgg ctggcacgtc tttggtgact
     2581 accgcacccg ctccgatgat cgcctcatcg ccgatcgtga cgcccatctg gatgatcgcg
     2641 ccggccccga tgaacacccg gtctccgatg acgaccggtg cgcgctccac cacgtcgaac
     2701 cgacgtccgc taacgcaccg cttcgccgag gaatgggtgt agacctgcgc ccccgtcgag
     2761 atgtcgcatc cggcgccgat cgtgaggcca ccggaggcgt caatcatcgt gaacgccccg
     2821 atccaggtgc cttccccgac gacgggctcc cccaccaccc aggcgtgttc gttccacggg
     2881 ttgggcggca gcgccgccgg ccttggggcc atcagccgcc gtcccggatg agggcggtca
     2941 tcccctcgcg caccggaatc gtcgcctcga acccgagcac ctcacgggcc cgggtgacgt
     3001 cagccgcccg tcggctggca atgacatcgc ggccgctgaa ctgcggttga acgtcgaccc
     3061 ccaccgcatc gatgaggatc cgcgccaact cggccacggt cgtgtcgatc ccggtgccga
     3121 tgttgacggg cacgttgtcg ccggcacagt cgagcgccag cacgacggac cgggcgatgt
     3181 cgtggacgtg gatgaagtcc atcgactgct cgcccttgcc gtcgatgacc ggcggctcgc
     3241 cggccttgag ccggttgacg aagtggttga tgaccgacgt gtagtaggcg gtggtcttct
     3301 ggccggggcc gtaaacgttg aagaaccgca aggcgatcca ggagagcccc gatcgccgct
     3361 ggtagaagtc cagcaggtct tcgccggtgc gcttggagat gcagtagggg gtctgcggcg
     3421 acaactcgtc gtcctcgtgc atgggcagtc gcttcgggtc gccgtagacc gacgcagacg
     3481 agcagaagac cagccggcgc acaccatgat cagccgccgc ggcgatgacg ttatggcttc
     3541 cgatggtgtt gatagccatc gactcgtgcg ggtcggcctg gctcttgttg atcgatacgg
     3601 ccgccgcgtg gatgacgacc gagcacccgg ccatcgccgc gtggacggca ccgccgtaac
     3661 ggatgtcctg gtcgatcagg tcgatccgcc cggggtgggc agcctggagt tgctcgacgg
     3721 cagaccggtc cccgcggaac atgttgtcga agatccgcac gttgtagcca cgctccagga
     3781 gcatcggaac gacgtggaga ccgatgaagc cggcacctcc agtgaagaag acggtctcgg
     3841 cgctcatgag gctgctggat cctttcgtat gccgcgtgcg cccttcccgc aggtcaggcg
     3901 cccgcgaggt ggctagcggc ggacggcgtc cgagccgacg acggctcgaa cggtgtccac
     3961 gacgtgggtg aggtcatcgt cagtgaggtt ggcgtgcatg ggaatcgcca gatggcgatg
     4021 gaacaggtcg gccgagaccg ggcactcgcg accggccggg gtgccataca cgggctggac
     4081 gtgcgaggca taggtgccga agttgcaccc caccccctgc tcgcgcagcg ccagcgcgac
     4141 ccggccacga tcgaggctcg ggtcgagggt gaggacgtac gactgccacg ggtgggtgcg
     4201 gtcgtccagc gcgacaggga gggtgagttg gtcgaggtcc ccgagccccg cggcatacac
     4261 ctgggccgac cgggtacgcg cggcgaggag gtcgggcaaa cggcccaact ggacccgcat
     4321 gatggcggcc tggacgtcgg agagtcggta gttgtagccc gcttcgtcga agctcgggac
     4381 gggcagggtc gcctcgccct cgcgcgtgac cgcgggcgcg atgccgtagg tgtgcagttt
     4441 gcgggcctgg tccatcaggt cgccccgatc cccaaccagc gcgccgccct cgcctgccgt
     4501 gatgcccttg cggccgtgga agctgaacgc cgcgagatcg gccaggctcc cggccggacg
     4561 gccgtggtag gtcgctcccg cggcgcacgc agcatcctcg acgatccaga ggccgtgccg
     4621 gtcagcgatg gcccgcaact cgtcgtagtc cgcgggctgc ccgaagacgt cgacggcgag
     4681 gatcccgacg gtgcgcggcg tgatcgctgc ctcgaccgcg gccgggtcgg ccgaccagat
     4741 gtcgggccgg acgtccgcga agacaggggt cgcgcccgtc cacaccacgg cgtgaccggt
     4801 ggcggggaag gtgtagtcgc cgacaatgac ctcgtcgccc ggtccggcgc cgaggacctg
     4861 cagggccagg tgcagggcgg agccgcagtt ggcggtcgtg agggcgtggg cggtcccgac
     4921 cgcccgagcg aactcgttct cgaaggccac gcacgtcggc ccggccccgg acaaccatcc
     4981 cgaggcgaac acctcggcga cggcagcaag ctcgggctcc ccgaccgtcg gttggccgag
     5041 gggcacggtg cgttgcatgc agactccttc gggttcgggc cagatgctac cagcgggggg
     5101 tatgccgccc tcccgcccag cgggtcgctc aggcgcgtcc gagcaactcg gagaaggtgg
     5161 cgaggtgggc gtcgagcacg cggtcgaggg tgaagtgcgc gtcgatgagg gcccttcctc
     5221 gtgcgcccac cgcctgcgcc tccaccgggt cgtcgagcac acgtagcagt cgctgcgcca
     5281 acccgacgac gtcaccccgg tcggtgagca gcacctcgcc gccatcgtga agcggcaccc
     5341 cggggaagtt gtccagccgc ccccacccga cgatcgggac gcccgccgcc atcgcttcca
     5401 acgtcgctgt ccccatcccg tcgccttgct catgactctc gagcgtcgcc gccgcaagca
     5461 ggtgcgggat gctcgctttg ggaaccgcgc caagggggcg cacggcatac gccacgccga
     5521 gctccgttgc gcggcggagg aagacgtcgt agtagacccg ccccgcgacg acgagccgcg
     5581 cgtcggggtg gcgcgcgagc accgcgggca gggcctcaac caagcccacc cggtcgcgca
     5641 gcgggatcac atgcccgacc gacaagagga tgggcgcgtc gccgaccccg gccagctcgc
     5701 gacccacggc cgcgtcgccg ccacggaccc acacggggtc caccccgacc gggatcggga
     5761 caagctcgcg gtatccacct cggtagcgag cggtgatgta gtcacgcatg tgggcatcca
     5821 tgacaacgat ccgcggccgg tagcgccgca gtcgcggcgc gacgagggtc gcgtcgagag
     5881 tgcggaaagc atgacggtag agggccgcgg ggttctccaa tcgggtgtgc accgagagga
     5941 gaacgggtat gccgcgccgc cgggcgtagg cgcccgtggc ccaggtgagg tccatgaact
     6001 ggccatgttg gtggatgacg tcgggtcgga aatcgtcgag cagccgggcc aggtcgcgcg
     6061 gcaacgacgg acggctcgcg aaggacaggt cgaagctcac cgcgaaccgg gtctccggca
     6121 acatcgctgc cgggaaacgc acgatccgga tgccgtcgcg ctgctcctgg gccggagcct
     6181 cgccgtaggc cgcggtgacg acgaggacct catgtgcgcg ggcggcatac cccctggcaa
     6241 gggcgtcgga gagatgcgaa gaaccgccga cccgcggagg gaagaagttg ttgacgacga
     6301 cgatccgcat ccggggcagc tacccgagcc tcgggactga ggaactcgcc cgggaccgac
     6361 tctgacctgt ccgcattcca caaggctaac ccaccgcggt tgcgcccctg ctggcgccgg
     6421 ccgacgtcac aggagggcct ctgcgagaga gttgccgcgc cgacacgccg tccgacacgc
     6481 cgactgatat cactttgtta tgtctcgcct gtggcgagca gccgcaacca tggtcaactt
     6541 ctgtgactga cgtgattgga gtgtttcatg gtgcgcaaga agcgtttggg aacgatgagg
     6601 gtgacgatcc gcgttctcac ggcagcggca gtggctgtcc tcgtcggagc tgtggttcct
     6661 tcggcgaacg cagctcag
//
LOCUS       DAOMZY010000194         6607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_10900_length_6607_cov_5.973748, whole genome shotgun sequence.
ACCESSION   DAOMZY010000194 DAOMZY010000000
VERSION     DAOMZY010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6607
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10900_length_6607_cov_5.973748"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..1231
                     /gene="alr"
                     /locus_tag="PLK64_11080"
     CDS             41..1231
                     /gene="alr"
                     /locus_tag="PLK64_11080"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPV80335.1"
                     /translation="MPLPPTHPSRRHRDMSAAVSVSLRPAAPTAGAVLDIDLSAVGAN
                     TRRFAALTPALLAVVKADGYGLGATQIARTALSHGAIGLGVTSLAEALALRADGLTAP
                     ILSWLNPVDGDFAAGLAAGVHLAVGSIEHLDAVCRAARRTGRAASVHLAVDCGMARDG
                     SPPLAWPALCARAGQAQQSGLVDVVGVMGQLSHAGRPRHAASQRGRAVFDWAVTTAAG
                     HGLAPEHRHLATTAACLTDPRSMHTMSRVGAGLVGVDPSSTTLLAQVVTLRAPVVAVR
                     DVVAGTGVGYGHTWRAKRATRLVTVPVGYADGIPRGAAGRAEVSVGGHRCPVVGGVNM
                     DQTVIDVGRLEIELGEPVVVLGGEGPTLVEWAEWAQTLPHEILTGLGGRVARRYSGVS
                     RDQR"
     gene            1228..2193
                     /locus_tag="PLK64_11085"
     CDS             1228..2193
                     /locus_tag="PLK64_11085"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPV80336.1"
                     /translation="MTRVAVIGGGRSSEHEVSLASAAAVADGLEQAAYDVLRLTITRD
                     GAWLADTPLPLAAAVAAVQECDVVFPAVHGPHGEDGTLAALCEVAMVPYVGCGVGAGA
                     IGMHKWATQLVAESVGVATARGRLLTDDGPIPWTGPCVVKPLRAGSSHGVTLVERPGD
                     LGAAVKAAFRCDTTVLVEQFVVGREIDLGVIEQPDGTWTVLPPLEIGRSGIFDTTTKY
                     DGSADFRVPAALADADRHALDDAALQVVDALGSRGLARVDFFLTEAGPILNEVNTMPG
                     LTPHSQLPRMASAAGLDYPDLLDLLVTTAVASRQRGRVPAVAAQC"
     assembly_gap    2294..2393
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2394..3631
                     /locus_tag="PLK64_11090"
     CDS             <2394..3631
                     /locus_tag="PLK64_11090"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPV80337.1"
                     /translation="TRHVRRWWPDAALGVLVASAGAFEMSARSIGRWWIVAVLAVAVG
                     LFRRAPVAAAALLWVLLYAHLRTGSTLLITEVTTALVAYGAARWGRTVTVWFSGLSIL
                     ALGVLGVWLVGTRLDIVLPLVPLVDAATLKRVLVDPPGVGTQVALIPLGLALVGFGPP
                     WLLGLAVRSIDEARRSRALQHLAEDHAALAHQQVEREREIAELRAGQARLARDVHDVV
                     GHSLAVILAQAQAAAYLQDPDEVRATVDRIADAARRSLQDVRTVLESTQVEGAAVRAQ
                     RPPGTLDALVTGLREAGHGVESTVIGVPQPLPPELETVAYRVAQEMLTNAVRHGDRRE
                     PVHVEQVWSTHSGDGAGQLRLEVRNTVAADAAPPQRPGLGVDGMVRRLASVGGSLDAR
                     LHDGWFVATASLPVRAVLR"
     gene            3648..4406
                     /locus_tag="PLK64_11095"
     CDS             3648..4406
                     /locus_tag="PLK64_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPV80338.1"
                     /translation="MSAEVARVDETNEVDHVDQLDPESPDVPDSADAAAPIRVLLVDD
                     QELFRAGVAVVVGADPGIDVVGEASDGREAVALVDALEPDVVLMDIRMPELDGVEATR
                     QIFAPERAARRPRPVRVIVLTTFNLDDRAATAIRYGASGFLLKDATPDVLREAIRTVH
                     LGNAVLSPADLGSLLEGDFRRRSATPAAYSQLTEREREVFAGVARGLSNAEIASTVFV
                     SESTVKTHVGAILRKLDLRDRVQIVVFAYEHALV"
     assembly_gap    4425..4524
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4525..5117)
                     /locus_tag="PLK64_11100"
     CDS             complement(<4525..5117)
                     /locus_tag="PLK64_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HPV80339.1"
                     /translation="MSPAKKHGTRYRPRRSVLYMPSSNERALEKAKSLPCDALILDLE
                     DAVAPDAKEVARDAACAAVQSGEYGRKELIIRVNALGTDWHYPDMMAACKAGPAGILV
                     PKVNSAKEVHDLVREMERYKTPKHTKLWAMIETPRSVLDVGRIAAASDRLAVLVMGTN
                     DLVKELDAQHLPGRVPIVPALALAVLSAKASGLFIIDG"
     gene            complement(5208..6293)
                     /locus_tag="PLK64_11105"
     CDS             complement(5208..6293)
                     /locus_tag="PLK64_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HPV80340.1"
                     /translation="MRHPKDFYAPLAVGAPMPMRAVPARPSRVIHFFDPSNAKMAAKI
                     PEMVGTVDVLLGNLEDAVKADNKEAARAGLVAIGKSTDFGDLTQFWTRINSLDSPWAL
                     DDLTTLVSEIGDKLDVIMVPKVQGPEDIHYVDRLLAQLEAKAGLTRPILVHAILETAR
                     GMANVEEICFASPRMQGLSLGPADLAADRRMKTTRVGGGHPGYLVREDPPGGDINGPR
                     AIFQQDLWHYTIARMVDACAMAGIFPYYGPFGDIKDVVACEDQFRNAFLLGCVGAWSL
                     HPAQIAIGKKVFSPSAADIAHARRVVAAMGDGTGAVMLDGKMEDDASLKQCLVMIELA
                     ERLAKTDPELASLYAAAPDPAAATSAG"
     gene            6408..>6607
                     /locus_tag="PLK64_11110"
     CDS             6408..>6607
                     /locus_tag="PLK64_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HPV80341.1"
                     /translation="MSLPKRVFLAQLAGLPVFDPLGDQVGRVNDIVVAMPTGYAATPT
                     GYAATPTTPRVIGLVVEVPGRKR"
BASE COUNT          880 a         2245 c         2274 g         1008 t
ORIGIN      
        1 tggaggtgcg gagaccgggg acgtccctag cgtcgaggtc atgccacttc cccccaccca
       61 cccgtcccgt cggcaccgag acatgagcgc ggccgtcagc gtttccctcc gtcccgccgc
      121 tcccaccgcg ggcgccgtcc tcgacattga cctgtcggcg gtcggcgcca acacccggcg
      181 cttcgcggcg ctgacgccag cgctgctcgc cgtcgtcaag gccgacggct acgggctggg
      241 tgccacgcag atcgcgcgaa ccgcgctgtc ccacggggca atcggcctcg gcgtgacctc
      301 gctggccgag gcactcgcgc tgcgcgcgga cggcctcacc gcaccgatcc tcagttggct
      361 caaccccgtg gacggggact tcgccgccgg cctcgccgcc ggggtgcacc tcgccgtcgg
      421 ctcgatcgag cacctcgacg cggtatgccg tgccgctcgc cgcaccggtc gggccgcgtc
      481 cgtccacctg gccgtcgact gcggcatggc ccgcgacggg tcgccgccgc tggcctggcc
      541 cgccctctgc gcccgggccg gccaggccca gcagtccggc ctggtcgacg tcgtcggagt
      601 catgggtcag ttgtcgcacg ccgggcggcc ccggcacgca gcctcccaac gcggccgggc
      661 cgtctttgac tgggcggtga cgactgcggc tgggcacggc ctcgctcccg agcaccgcca
      721 cctcgccacc accgccgcct gcctcaccga cccccgatcc atgcacacga tgagccgggt
      781 cggggccgga ttggtcggtg tcgacccctc ctcgacgacc ctgctggctc aggtcgtcac
      841 gctgagagcg ccggtcgtcg cggtgcggga tgtcgtggca ggcaccggcg tcggctacgg
      901 ccatacctgg cgggcgaagc gggccacccg actggtcacc gttccggtgg gctacgcgga
      961 cggcatacct cgcggtgctg ccgggcgtgc ggaggtgtcg gttggtggcc accgctgccc
     1021 ggtcgtcggt ggggtcaaca tggaccagac ggtcatcgac gtcgggcgac tggagatcga
     1081 gctcggggag cccgtggtcg tcctcggcgg cgaagggccg acccttgtcg agtgggccga
     1141 atgggcgcag acgctgccgc acgagatcct caccggcctg ggcggccgcg tggcgcggcg
     1201 gtattcgggt gtctcacggg accagcgatg acccgggttg ccgtcatcgg cggtggccgc
     1261 agcagcgagc acgaggtgtc gctcgcctca gccgcagcag tggccgacgg actggagcag
     1321 gcggcatacg acgtcctgcg cctgaccatc acgcgcgacg gtgcctggct ggcggacacg
     1381 cctctgccgc tggccgccgc ggtcgccgcc gtgcaggaat gcgacgtcgt gttccctgcg
     1441 gtccacgggc cgcacggtga ggacggcacc ctcgcggcac tgtgcgaggt cgcgatggtc
     1501 ccctacgtcg gttgcggggt cggggcggga gcgatcggga tgcacaaatg ggcaacccaa
     1561 ctcgtcgccg agtcggtggg cgtggccacc gcccgggggc gcctgctgac agacgacggg
     1621 ccgatcccgt ggaccggacc ctgcgtcgtc aagccactgc gggccgggtc cagtcatggg
     1681 gtcacccttg tcgagcgccc gggtgacctc ggggcggcag tgaaagcggc tttccgttgc
     1741 gacacaacgg tgctcgtcga gcagttcgtc gtcggtcgag agatcgacct cggggtgatc
     1801 gaacagcccg acggcacctg gaccgtgctg ccaccgttgg agatcggccg gtcgggcatc
     1861 ttcgacacga cgacgaagta cgacggcagc gccgacttcc gggtcccggc ggcactcgcc
     1921 gacgcagacc gacacgctct ggatgatgcc gcactgcagg tcgtcgacgc cctcggcagc
     1981 cgcggcctgg cccgtgtgga cttcttcctc accgaggccg gtccgatcct caacgaggtg
     2041 aacaccatgc cggggctcac gccgcattcc caactgcccc ggatggcctc ggcggccggg
     2101 ctcgactacc ccgacctgct cgacctgctc gtcaccaccg ctgtcgcgag ccgccagcgt
     2161 ggccgcgtcc cggccgtcgc ggcgcagtgt tgactaggca ccgtgccatt cccagacacg
     2221 ccgccacggc tgggccagtg acccgccacg tgcggcgctg gtggcccgac gccgccctcg
     2281 gcgtcctggt cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgacccg
     2401 ccacgtgcgg cgctggtggc ccgacgccgc cctcggcgtc ctggtcgctt cggcgggtgc
     2461 tttcgagatg agtgcgcggt caatcggtcg ttggtggatc gtcgccgtcc tcgcggtcgc
     2521 ggtcgggctg ttccggcgcg caccggtggc ggccgcggcg ctgttgtggg tcctgctcta
     2581 cgcgcacctg cgcactgggt ccaccctgct gatcaccgaa gtgacgacgg cgctggtggc
     2641 ctatggggcg gcccgctggg gacgcaccgt cacggtctgg ttcagcggcc tgtcgatcct
     2701 tgcgctcggt gtgctggggg tctggctggt cggcacccgg ctcgacatcg tcctgccttt
     2761 ggtgccgctc gtcgatgccg caacgctcaa gcgggtgctg gtggatccgc ccggggtggg
     2821 gacccaggtc gccctcattc cgctggggct cgccctcgtc ggcttcggcc cgccgtggct
     2881 gctcggcttg gcggtgcgat cgatcgacga ggcacgccgg tctcgcgccc tgcagcacct
     2941 cgccgaggat cacgccgccc tggcccacca gcaggtcgag cgggagcggg agatcgccga
     3001 gttgcgcgct ggccaggccc gcctggccag ggacgtccac gatgtcgtcg ggcactcgct
     3061 ggcggtgatt ctcgcgcagg ctcaggccgc ggcatacctg caggatcccg acgaggtccg
     3121 ggcgacggtc gatcggatcg ccgacgcggc gcgccggtcg ctgcaggacg tgcggaccgt
     3181 gctggagtcg acccaggtcg agggtgccgc cgtccgagca cagcgacccc ctgggacgtt
     3241 ggacgccctc gtgacaggct tgcgcgaggc cgggcacggg gtggagtcga ccgtcatcgg
     3301 tgtcccccaa ccgctaccgc ctgaactcga gacggtcgcc taccgggtcg cccaggagat
     3361 gctcaccaac gcggtgcgac acggcgatcg ccgtgagccg gtccacgtcg agcaggtctg
     3421 gagcacacac tccggcgacg gcgcgggtca actgcgactg gaggtgcgca atacggttgc
     3481 tgccgatgcc gccccgcccc agcggcccgg cctgggggtc gacgggatgg tgcgtcggct
     3541 ggcctcggtg ggcggcagcc tggacgcccg cctgcacgac ggttggttcg tcgcgaccgc
     3601 gtcgctgccg gtgcgggcgg tcctgcgatg acggtcgagg tccgcggatg agcgcagagg
     3661 tggcccgggt ggacgagacg aatgaggtgg accacgtgga ccaactggac ccggagtccc
     3721 cagacgtccc ggattccgcc gatgcggccg cgccgatccg cgtgctgctc gtcgacgacc
     3781 aggaactctt ccgggcaggg gtcgcggtcg tcgttggcgc cgacccgggg atcgacgtcg
     3841 tgggcgaagc cagcgacggc cgcgaagccg tggcgcttgt cgatgcgttg gagccggacg
     3901 tcgtgctcat ggatatccgg atgcccgaac tcgacggagt ggaggcgacc cggcagatct
     3961 tcgctccgga gcgggccgcc cgccgccccc gcccagtgcg agtcatcgtc ctgacgacct
     4021 tcaacctcga cgaccgggca gcaacggcaa tccgctacgg cgccagcggt tttctcctca
     4081 aggacgccac acccgacgtc ctgcgcgaag ccatccgcac cgtccatctc ggcaacgccg
     4141 tactctcgcc cgccgacctc ggcagccttc tggagggcga cttccggcga cgcagcgcca
     4201 cgcccgcggc atactcccag ctgaccgagc gtgagcgcga ggtcttcgcc ggtgtcgccc
     4261 gcgggctgtc caacgccgag atcgcgtcga cggtcttcgt cagcgagtcg acggtcaaga
     4321 cccacgtcgg ggcgatcctg cgcaaactcg acctccgcga tcgggtgcag atcgtcgtct
     4381 tcgcctacga gcacgccctc gtctgaggtc gcgctcgctg ggccnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnccgtcg atgatgaaca gcccggacgc cttagccgac
     4561 agcaccgcga gcgcgagcgc cggaacgatc gggacccggc cgggaaggtg ctgggcatcc
     4621 aactccttca ccaggtcgtt cgtacccatg accaacacgg ccaaccggtc ggaagccgcg
     4681 gcgatccggc cgacgtccag gaccgaccgt ggggtctcga tcatcgccca gagtttggtg
     4741 tgcttcggcg tcttgtaacg ctccatctcg cgcaccaggt cgtgaacttc cttggcggag
     4801 ttgacctttg gcacgaggat ccccgcaggg cctgccttgc aggcggccat catgtcgggg
     4861 tagtgccagt cggtgcctag cgcgttgacc cgaatgatga gttccttgcg gccgtactca
     4921 cccgactgga cggcagcgca cgcagcgtcc cgcgcaacct ccttggcatc gggcgcgacg
     4981 gcgtcctcaa gatcaagaat caacgcgtca cacgggaggc tcttggcctt ctccagcgcc
     5041 cgctcgttgg aggacggcat gtagaggacc gagcggcgcg gccggtaacg ggtgccgtgc
     5101 ttcttggctg gggacatcgg tggctccttg ggtggccgag tggtcaggtg cggtgattcg
     5161 agtggcggtg cgcgggtggt gcgatgggcg ccgagcaggt atgccggtca gccggctgac
     5221 gtcgcggccg ccggatcggg ggcggcggca tacaggctgg ctagctcggg gtcggtcttg
     5281 gcgaggcgct ccgcgagctc aatcatgacc aggcactgct tgaggctcgc gtcgtcctcc
     5341 atcttgccgt cgagcatcac ggcgccggtg ccatctccca tggcggcgac gacccgacga
     5401 gcgtgggcga tgtcggcggc cgacgggctg aagaccttct tgccgatggc gatctgagcc
     5461 gggtgcagcg accaggcgcc gacacaaccc agcaggaacg cgttgcggaa ctggtcctca
     5521 caggcgacga cgtccttgat gtcaccgaac gggccgtagt aggggaagat cccggccatc
     5581 gcgcaggcat cgaccatccg ggcgatcgtg tagtgccaca ggtcctgttg gaagatcgcg
     5641 cgggggccgt tgatgtcgcc gccgggcggg tcctcgcgga cgagatagcc cgggtgcccg
     5701 ccaccgaccc gggtcgtctt catccgccgg tcggcggcca ggtcggcggg gccgagcgag
     5761 agcccctgca tccgcggcga ggcgaagcag atctcctcga cgttggccat gcctcgggcc
     5821 gtctcgagga tcgcgtgcac gaggatcggg cgggtgagcc cggccttggc ctcgagctgg
     5881 gcgagcagcc ggtcgacgta gtggatgtcc tcggggccct gaaccttggg caccatgatg
     5941 acgtcgagtt tgtcgccgat ctcgctcacg agcgtcgtga ggtcatcgag cgcccagggg
     6001 ctgtccagcg agttgatccg ggtccagaac tgagtcaggt caccgaaatc cgtggacttg
     6061 ccgatcgcga ccagcccggc ccgcgctgcc tctttgttgt cggccttgac ggcgtcctcg
     6121 aggttgccga gaagcacgtc gacggtcccc accatctcgg ggatcttggc cgccatcttg
     6181 gcgttgctcg ggtcgaagaa atggatgacc cgcgacgggc gagcgggcac cgcgcgcatc
     6241 ggcatcggag cgccgacagc caacggtgca tagaagtcct tggggtggcg catgacactc
     6301 cttcacgaag cggcgaccgg ccgctcttcg gtgaacacgt ccagaaccga cgctagcagc
     6361 gcaccgcgca cgaccgttat gacgtcggcc acgcgtaggc tcacgctgtg agtctgccca
     6421 agcgggtttt tctagcccaa ctcgccgggc tgccggtgtt cgaccccctg ggagaccagg
     6481 tgggtcgggt gaacgacatc gtcgtggcca tgcccaccgg gtatgccgcg acccccaccg
     6541 ggtatgccgc gacgccgacc acgccccggg tcatcggctt ggtcgtcgag gttcctggcc
     6601 gcaaacg
//
LOCUS       DAOMZY010000195         6602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_10916_length_6602_cov_5.646556, whole genome shotgun sequence.
ACCESSION   DAOMZY010000195 DAOMZY010000000
VERSION     DAOMZY010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6602
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10916_length_6602_cov_5.646556"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..2064)
                     /locus_tag="PLK64_11115"
     CDS             complement(25..2064)
                     /locus_tag="PLK64_11115"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HPV80342.1"
                     /translation="MNFFSIYDHGFARVAACTVPVRLVDPAANVAATLAVARECHDEG
                     VAVAVFPELGLTGYSLDDLVLADPLLDAVEVALEELVRASAQLLPVLVVGAPLRARGR
                     VYNCAVVVHRGRVLGVAPKSYLPTYREFYESRTIAAGDDVRGTIRVGEDNVPFGPDLL
                     FRAVDVPDLVVHVEVCEDMWVPIPPSAEAALAGATVLVNVSGSPITVAKARDRAMLAE
                     SASSRCLAAYLYAAAGEGESSTDLSWDGQTMVWENGSLLAEGERFPSGPRRSVADVHL
                     ARLRQERMRQGTFDDNRRTHAARVGAFREVTFRLDPPRGDLGLRRAVDRYPFVPDDAA
                     RLAQDCFEAYSIQVSGLEQRLRSIGRPTVVIGVSGGLDSTHALIVACRAMDRLGRPRS
                     DVLAFTMPGFATSDHTKGNAIRLMESLGVTWEELDIRPAATQLLRDLGHPVGRGEEVY
                     DVTFENVQAGLRTDYLFRIANQRKGIVLGTGDLSELALGWCTYGVGDQMSHYGVNTGV
                     PKTLIQHLIRWVIASGQFSDEVGATLRSILDTTITPELVPTREGEAAQSTEAMIGPYA
                     LQDFTLYYVLRHGLRPSTIAFLAWHAWHDTASGQWPEGFPEGARTAYTLGEIRHWLEV
                     FLTRFFANQFKRSALPNGPKVVRGGSLSPRGDWRMPSDASAKVWLDELHSTVPEA"
     gene            2312..3556
                     /locus_tag="PLK64_11120"
     CDS             2312..3556
                     /locus_tag="PLK64_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HPV80343.1"
                     /translation="MPPAPAPSRGPRFEPFRALRYAPGIDLDAVIAPPYDVLSDADVA
                     ALRARDPHNIVHADIPAGTDSTRYAASAHLLRDWQETGVLVRDEVPTLSIYRMRFVDE
                     AGQRRELVGVLGGLEVVAEGAGQVLPHERTTPKASTDRLDLTRATGTNLSPVWGLSLA
                     QGLTAALAEPAEPMGSLVVDGVEHVVERVIDTDRIAAISAIIGADDVLIADGHHRYGV
                     ARRYRDEMAEAKEPESASGAGATLAFVGELVADQLAIDAIHRIVRGLTPAALREALAR
                     DFELVPAPAAQPGPALLAELNRSGRLLLIESPEVATWLVPRPGAFDDVRALDGAWLEH
                     TLAGTGAVLDYQHGVAEVLDVLHADPQALAILIRPTSLEQIERTAREGLLMPPKSTFF
                     TPKLRTGLVLRPLDEAGYVATA"
     gene            complement(3553..4617)
                     /locus_tag="PLK64_11125"
     CDS             complement(3553..4617)
                     /locus_tag="PLK64_11125"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HPV80344.1"
                     /translation="MSIVRRMSLAIGSLLVITVYGVVGYMILGYNLLDAVYQAVMTLT
                     TVGYGEVLEATPVRKAFSTSLMILGVGIALYNLTVMVGTVTEGDVRKYFERRRMDKRI
                     SGMSGHVIICGHGRVGRSAREHLKNIGSQVVVVDNVPDRVMHLEDPHLIGDCTRDEVL
                     RAAGIDRARALIATLETDADTVYLTLSARALRPDLVIIARARTVDSKEKLVLAGATRA
                     VNPQLIGGRRMAAFALNAHVAEFLDVVMHDEELDFRIEQIRIPVDSPLAGRSLGELDL
                     EQSSGARLLAHRPGPRMPFAPHPSSDTVLEPGSVLIAFGTMAQIEHLMDQASPDPGTL
                     GAGPAAPGGPVSPIIARTSA"
     gene            4806..6194
                     /locus_tag="PLK64_11130"
     CDS             4806..6194
                     /locus_tag="PLK64_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPV80345.1"
                     /translation="MGSADRDVSRVAIVGAGPSGLFAAQSLVGQSDLPVQVDLIDRLP
                     TPYGLLRYGVAPDHTSIKSVANALARTFDSNGVRFIGNVELGRDISREQLLAAYDAVV
                     YAAGAAEDAQLGVPGEDLAGSRSAREFVAWYSGHPDAPAQDLGGVRTAAVIGVGNVAV
                     DVARVLAKTADSLEVTDMPPAVLAELHRHQADDIHVIARRGPQHAAFTTVELRELVHT
                     PGLAVSVNEDAFDGIPDDLERRPKANVEILREAAATVVENPRRRLHFWFWRKPIAIEG
                     DGRVEAIVLERTRLDETGKVVGTGETQRLETELVLRSIGYRSTALAGVPFDERAAVIP
                     NVEGRIVGADGEVLPREYVVGWIKRGPTGVIGTNKSDASQTVRHLVADLVAARAGDGG
                     AAGGLTGGVTGADRPDLLTALAEQGHEVISLDGWRAIDAAEIARGAALGRDRTKLDTW
                     SALLEAGRLDPV"
     gene            6246..>6602
                     /locus_tag="PLK64_11135"
     CDS             6246..>6602
                     /locus_tag="PLK64_11135"
                     /inference="COORDINATES: protein motif:HMM:NF039605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5956 family protein"
                     /protein_id="HPV80346.1"
                     /translation="MFIVELRDTGWDMLVACALGPGATFRAVREPDPHGMYALCDGGL
                     NVHVSEGELLEIEDGINAYLAALSLPPRPAGYRWFVALPRRLSRTEITQTLGGIAGSM
                     HPPFVAPCAPGQLDELC"
BASE COUNT         1033 a         2237 c         2325 g         1007 t
ORIGIN      
        1 gggggagcgg gattggcgga cgggtcaggc ttcggggacg gtgctgtgca actcgtcgag
       61 ccagaccttc gcggacgcgt cggacggcat acgccaatcg cctcggggcg acagcgaacc
      121 gccgcggacc accttcgggc cgttgggcag cgccgagcgc ttgaactggt tggcgaagaa
      181 ccgggtgagg aagacctcca gccagtgccg gatctccccc agggtgtatg ccgtgcgcgc
      241 accttcgggg aaaccttccg gccactgccc gctcgccgtg tcgtgccagg catgccaggc
      301 caggaaggcg atcgtcgacg gtcgcaagcc gtggcgcagg acgtaataca gcgtgaaatc
      361 ctgcaaggca tacggcccga tcattgcctc ggtcgactgc gcagcctctc cctcacgggt
      421 cggcaccaac tcgggcgtga tcgtcgtgtc gaggatcgag cgcagggtcg cccccacctc
      481 atcgctgaac tggcccgagg cgatgaccca ccggatgagg tgctggatga gcgtcttggg
      541 caccccggtg ttgaccccgt agtgcgacat ctgatcgccg acgccatagg tgcaccagcc
      601 cagggcgagc tcggacaggt cgcccgtgcc gagcacgatc cccttgcgct ggttggcgat
      661 tcggaacaaa tagtcggtgc gcaggccggc ctggacgttc tcgaaggtga cgtcatagac
      721 ctcctcgccg cgccccaccg ggtgcccgag atcgcggagc aactgggtgg ccgccggccg
      781 gatgtcgagc tcctcccagg tgaccccgag cgactccatc agccggatcg cgttgccctt
      841 cgtgtgatcc gaggtcgcga aacccggcat ggtgaaggcc agcacgtccg agcgtggccg
      901 ccccaaccgg tccatagccc ggcacgcgac gatcagcgcg tgggtcgagt cgagcccacc
      961 cgacaccccg atgaccaccg tcggtcggcc gatcgagcgc agccgctgct ctaggcccga
     1021 cacctggatc gaatacgcct cgaaacagtc ctgcgccaat cgggccgcgt cgtccggcac
     1081 gaacggatag cggtcgactg cccgacgcag cccgagatcg ccccgcggcg gatccaaccg
     1141 gaaggtcacc tcgcggaacg ccccgacccg cgcggcgtga gtgcgccggt tgtcgtcgaa
     1201 cgtcccctga cgcatccgct cctgccgcaa cctcgcgagg tgcacgtcgg cgaccgaccg
     1261 tcgcggcccc gacgggaagc gctcgccctc ggccagcagc gacccgttct cccagaccat
     1321 ggtctggccg tcccatgaga ggtcggtgct cgactcgcct tctccggcgg cggcatacag
     1381 gtatgccgcg aggcagcgtg aggaggccga ctcggcgagc atcgcgcggt cgcgcgcctt
     1441 ggcgaccgtg atcggcgacc ccgacacgtt gacgagcacc gtggctccgg ccagcgcggc
     1501 ctcggcggac ggtgggatcg ggacccacat gtcctcgcag acctcgacgt ggacgacgag
     1561 gtcaggcaca tcgacggccc ggaagagcag atccggccca aacggcacgt tgtcctcgcc
     1621 gacccgaatg gtcccgcgca cgtcgtcgcc ggccgcgatg gtccgcgact catagaactc
     1681 gcgataggtc ggcaggtaag acttcggcgc caccccgagc acccgccccc ggtgcacgac
     1741 gaccgcgcag ttatagaccc gccctcgtgc gcgcaagggc gccccgacga ccaacaccgg
     1801 caacaactgg gccgatgctc gcaccaactc ctcgagcgcg acctcgactg cgtcgagcag
     1861 tgggtcggcc agcacgaggt cgtccagcga gtagccggtc agtcccaact ccgggaacac
     1921 cgcgacggca acgccctcgt cgtggcactc gcgcgccacg gccaacgtgg cggcgacatt
     1981 ggcagccggg tcgacgagac gcaccgggac cgtgcaggca gcgacccgcg cgaagccgtg
     2041 gtcatagatg gagaagaagt tcatgcggct accgcgcgcc tccctggtcc ggcggccccg
     2101 ggtcggagcc cgtctcgagt ctcccacgac ccgagggcat aggtatgccg cgtgcggtga
     2161 ggccaggggc atggcccgag cgatggacac gagggggagc gccaccgttc gccgacacgc
     2221 ggcatacatg agcattgttg cccacagtct gcggttccgc atctcgaact gctcacggag
     2281 gcaggctcac tcgttgccta gacttccagg tatgccgccc gcacctgccc cttctcgcgg
     2341 gcctcggttc gagccgttcc gggctctgcg ctacgcgccc ggaatcgacc tcgacgccgt
     2401 catcgcaccg ccctacgacg tgttgtccga cgccgacgtc gccgcacttc gggctcgcga
     2461 cccgcacaac atcgtccacg cggacatccc cgcaggcacc gactcgaccc ggtatgccgc
     2521 ctccgcgcac ctgctgcgcg actggcagga gaccggcgtc ctggtccgtg acgaggtgcc
     2581 gacgctgtcg atctatcgga tgcgtttcgt cgacgaagcc ggccagcgac gcgagttggt
     2641 cggggttctc ggcgggttgg aggtggtcgc tgagggggcc ggtcaggtgc ttccgcacga
     2701 gcggaccacg cccaaggcgt cgactgaccg tctcgatctc acccgagcca ctggcaccaa
     2761 cctctcgccg gtctgggggc tgtcgctggc gcagggtttg accgctgcgc tcgccgagcc
     2821 cgccgagccg atggggagtc tggtcgtcga cggcgtcgaa catgtcgtcg agcgggttat
     2881 cgacaccgac cggatcgccg cgatctcggc gatcatcggc gccgacgacg tgttgatcgc
     2941 cgacggtcac caccgttatg gcgtggcccg gcgctaccgc gatgagatgg cggaggccaa
     3001 agagcccgag agcgcatccg gggcgggggc cacgttggcc ttcgtgggcg agctcgtcgc
     3061 cgatcagttg gctatcgacg ccatccaccg cattgtccgg ggcctgaccc cggcagcctt
     3121 gcgggaggcg ttggcgaggg acttcgagtt ggtgcccgcc cccgccgccc agcccggccc
     3181 ggcgctgctc gccgagctca accgctcagg gcggttgttg ctcatcgagt caccggaggt
     3241 ggcgacttgg ctggtgccgc ggccgggggc tttcgacgac gtgcgggcgc tcgacggcgc
     3301 ctggctggag cacacgctgg ccgggacagg ggcggtgctg gactaccagc acggggtggc
     3361 cgaggtgctc gatgtgctgc acgccgaccc gcaggccctg gcgatcctta tccgcccgac
     3421 cagcctcgag cagatcgagc ggacggcgcg cgagggcctg ctcatgccac ccaagtcgac
     3481 gttcttcaca cccaaactgc gcaccggcct tgtgctgcgc cccctcgacg aggctggcta
     3541 cgtcgcgact gcctaagccg acgtgcgggc gatgatgggg gagacggggc caccgggggc
     3601 ggcgggccct gccccgagcg tcccggggtc gggagaggcc tgatccatga ggtgctcgat
     3661 ctgggccatc gtgccgaacg cgatcaacac cgacccgggc tccagcacgg tgtccgacga
     3721 cgggtggggg gcgaacggca tacgcgggcc tggtcgatgg gcgagcaggc gcgcgccgga
     3781 cgattgctcg aggtccaact cgccgaggct gcgtccggcc aacggcgagt cgactgggat
     3841 ccggatttgt tcgatccgga aatcgagctc ctcgtcgtgc atgacgacgt cgaggaactc
     3901 cgcgacatgg gcgttgagcg cgaaggccgc catgcggcga ccgccgatga gttgcgggtt
     3961 gacggctcgg gtcgccccgg cgaggacgag cttctccttg gagtcgacgg tgcgggcgcg
     4021 ggcgatgatg accagatcgg gacgcagggc ccgcgccgag agggtgagat agacggtgtc
     4081 ggcgtcggtc tccagggtgg cgatgagggc gcgggcacgg tcgatgccgg ccgcgcggag
     4141 cacctcgtcg cgcgtgcagt cgccgatgag gtgcgggtcc tcaaggtgca tgacccgatc
     4201 ggggacgttg tcgacgacga ccacctgcga accaatgttc ttcaggtgct cgcgcgccga
     4261 gcgacccacc cggccgtggc cacagatgat gacgtggcca ctcatgcccg agattcgctt
     4321 gtccatccga cgcctttcga agtacttccg gacatccccc tcggtgaccg tcccgaccat
     4381 caccgtgagg ttgtagaggg cgataccgac cccgaggatc atcaggctgg tcgagaacgc
     4441 cttgcgcacc ggcgtcgctt ccaagacctc gccatagccc acagtcgtca gggtcatgac
     4501 cgcttggtaa accgcgtcga gcaggttgta gccgaggatc atgtaaccca cgacgccata
     4561 gaccgtgatg acaagcagcg acccgatcgc gagggacatg cggcggacga tgctcacgtt
     4621 ggctattgaa ccgcgtcccg ggcgttccgg atagcagcgg aggtatgccg cgcggcaggc
     4681 gactcggtgc cggcgcggca tacctcgtcc ggcgtgtggg tgggacctcg gtcacgcgac
     4741 gttcgcggcg gtgcccgtta ggctcgggcc gtcaacgtcg acgctcgtgg caccggaggt
     4801 gcgcgatggg cagtgcagat cgggacgtca gccgggttgc gattgtcggg gccggcccgt
     4861 ccgggctgtt tgccgcccaa tcgctggtcg ggcagagcga ccttccggtg caggtggacc
     4921 tcatcgaccg gctgccgacg ccttatggcc tgctgcgcta cggcgtcgcg cctgaccaca
     4981 cgagcatcaa gtcggtcgcc aacgcgctgg cccgcacctt cgacagcaac ggcgtccgct
     5041 tcatcggcaa cgtcgagctc ggtcgagaca tctcgcgcga gcagcttctc gcggcctacg
     5101 acgccgttgt gtatgccgcg ggcgcggctg aggatgccca gctcggcgtc ccgggggagg
     5161 acctggcggg cagccggtcg gcgcgcgagt tcgtcgcttg gtattccggg catccggacg
     5221 cccccgcgca ggacctcggc ggcgtgcgga ctgctgcggt gatcggcgtc ggaaacgtcg
     5281 cggtcgacgt cgcccgggtc ctggccaaga cggccgactc gctcgaagtg accgacatgc
     5341 ccccggcggt gttggctgaa ctgcacaggc accaggccga cgacatccac gtcatcgcgc
     5401 ggcgcgggcc gcagcacgcg gccttcacga cggtcgagct gcgcgagctc gtccacacgc
     5461 cggggctggc ggtgagcgtc aacgaggacg cgttcgacgg catacccgat gatctggagc
     5521 gccgaccgaa ggcgaacgtc gagatcctgc gagaggcggc ggcgacggtc gtcgagaacc
     5581 cgcgtcggcg gctgcacttc tggttctggc gcaagccgat tgccatcgag ggcgacggcc
     5641 gagtcgaagc gatcgtgctg gagcggaccc ggctggacga gaccggcaaa gtggtcggca
     5701 ctggcgagac ccagcggctg gagaccgagt tggtgctgcg ctcgatcggg tatcggtcga
     5761 ccgcgctcgc cggggtgccg ttcgacgagc gtgccgcggt gatccccaac gtcgaggggc
     5821 ggatcgttgg ggcggacggg gaggtgctgc ctcgggagta tgtcgtcggg tggatcaagc
     5881 gcggcccgac cggggtcatc ggcaccaaca agtcggacgc gtcgcagacc gtgcgccacc
     5941 tcgtggccga cttggtggcc gcgcgggccg gcgatggcgg ggcggccggt gggttgactg
     6001 gtggggtgac cggggcggac cgaccggacc tgctcaccgc gttggccgag cagggccacg
     6061 aggtgatcag cctcgatggc tggcgggcca tcgacgctgc cgagatcgct cggggtgccg
     6121 ccctgggtcg cgatcgcacc aagctcgaca cctggtcggc gttgttggag gcggggcggc
     6181 tcgaccccgt ctgatccccg gcaatggggt ggcggacaac cccctgagca cttctgggag
     6241 cgatcgtgtt catcgtggaa ctccgcgaca ccggatggga catgctcgtc gcgtgcgcgc
     6301 tcggtccagg cgcgaccttc cgtgcggtca gggagcctga tccacacggc atgtatgcac
     6361 tctgcgatgg tgggttgaac gtccacgttt ccgaagggga actgctggag atcgaggacg
     6421 gcatcaacgc ctacttggcc gctctttcct tgcccccacg cccagcgggc taccgatggt
     6481 tcgtggccct ccctcggaga ctgtctcgca cggagatcac gcaaacgctg ggaggaatcg
     6541 caggctcaat gcatccgccc tttgtggccc cgtgtgctcc cggccagctc gatgagctct
     6601 gt
//
LOCUS       DAOMZY010000196         6586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_10949_length_6586_cov_4.714592, whole genome shotgun sequence.
ACCESSION   DAOMZY010000196 DAOMZY010000000
VERSION     DAOMZY010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6586
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10949_length_6586_cov_4.714592"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            416..805
                     /locus_tag="PLK64_11140"
     CDS             416..805
                     /locus_tag="PLK64_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV80347.1"
                     /translation="MDVAPDQGAAMLRLDLSRREEVMLTTAQVAKDLGKAKSTITRQV
                     NRGDLYAIKGDGQLRLPAWQFVSGAAVPGLAQALAYVPETWGPRRLRLFMTTPEEALG
                     DRSPVQWLLDNEPPQVVAQLMADESRE"
     gene            802..1455
                     /locus_tag="PLK64_11145"
     CDS             802..1455
                     /locus_tag="PLK64_11145"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HPV80348.1"
                     /translation="MTPKPQPKTPRKPPKPLTRRSSDLVGVRPTLWRIGATIGDYVLE
                     WNEARDFGPLRTMRWDPHHPPQSVQPDRAVIYASPSPITAVAEKFQKYRTIDPFTAAP
                     CLYGWSATRQLTLLDLRGNWPINNKGSASMPMGPRNVCRAWSAAILDTWPELDGLLTR
                     STMDGRDTYVLFPPARTSMPDEPRHARLLTDPAVYCYLDLWADEINYDIADAPWPPA"
     assembly_gap    1520..1619
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1620..2134)
                     /locus_tag="PLK64_11150"
     CDS             complement(<1620..2134)
                     /locus_tag="PLK64_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPV80349.1"
                     /translation="MLLKDKIAVITAGASGMGQASAQLFAEHGAHVIVVDVNEAAATA
                     TVEGIIAAGGSAEAAAVDVRDLDALERLGAKITADHGKVHILYNNVGIPGAAGMQLSE
                     ADWDTGIEINARASFFLTGHLLDALKAADGASVIFTSSTSGLVGSPFSPLYSFTKGGI
                     IALVRSYALALA"
     gene            complement(2211..4196)
                     /locus_tag="PLK64_11155"
     CDS             complement(2211..4196)
                     /locus_tag="PLK64_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPV80350.1"
                     /translation="MTIEPAGQHFPHLFAPITVGTMRLRNRVMLPPHASAIGNIYGTD
                     DEAARNIAYFEERARSGVAWIASLSTHIRNHLIPGFDPTGVGAATTGFFRLPFFVERV
                     QAFSDACHALGATVSVQCVHQGGMPHAPSARLSAPVINLVPHVMTHADIAEFVREYAE
                     SARLSMLGRADGFELHLNHDDLHEWFLSPYTNHRTDRYGGSLENRCRFATEVLQAVRD
                     VVGTSMTVGIRLNIRQEAPEGFGVAGGIELAQYFESTGLIDYVHGVVGSPWGNPSYIQ
                     PTYIDAGAFGELAGALKAAVSLPVVHTGRINAPEVAERILAAGQADVVGMARAHIADG
                     QLLVKAREGRVAEIRPCVGGNECISRRYVEGLGFACAVNPATSHEVEGPLPQAVSPRE
                     VLVVGGGPAGMELSALLAESGHRVQLWEAQDRLGGALHYATLAPRHDMYAAYLDWQVA
                     RLATAGVEVSLGRRATADDVLAVGADTVAIATGATPRRPGILGDDLPHVLDVRDVLAE
                     RAIPGRRVLVVSQDDHVAPLSVADFLSGRGHEVTVVFATATPAPLLWRYIVGAILGRL
                     DEQGVELRSMEEVTAIEAGRVHTRNVYSLRPRVIKDVDSVVLACGSVSDSTLCDDLAG
                     RHTDLHILGDAYAPRRLVFATKQAYALADLIARRPSA"
     gene            4367..5242
                     /locus_tag="PLK64_11160"
     CDS             4367..5242
                     /locus_tag="PLK64_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPV80351.1"
                     /translation="MPARQTSGGSLLLVMAAVVSVQFGGAVAATLVPVIGAAGSVVLR
                     LGIGAVVLLAVARPTLRGHSASAWRTVVLFGLVLGAMNWSFYASLDRLHIGVAVTIEF
                     IGPLTLAAVQSRRVRDLLAVAAAAAGILLISQALTVPWGDLDLIGIGLALLAGTFWAG
                     YIVLSQRTGAAFPGLNGLALAMIVALALVLPFGIWSVPLWTGDVLLKGAAVAILSSVL
                     PYSLELLALRQLSQRVFGILLSLEPAVAALAGLIVLGQRLTPTQLVGMALVVVASGLV
                     MGAGGRHTPADGAAT"
     gene            complement(5296..5877)
                     /locus_tag="PLK64_11165"
     CDS             complement(5296..5877)
                     /locus_tag="PLK64_11165"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPV80352.1"
                     /translation="MNVNDVTRTIMQCRPDAILVSSLGTATSSVRLVTSDGPHFYFGA
                     AMGSALAGALGLAEAVPDRQVVAVLGDGEFLMGASALWSVAAYRPSNLTVVVLADGAY
                     SITGGQPIPAPLHVAAVANALAETRGVTVSSTTELAATLRAEPTSPDAPGAPLVIEAT
                     ITERTWPGPSPFIDPAATVRNLRASLAQAPAPA"
     gene            complement(5874..6425)
                     /locus_tag="PLK64_11170"
     CDS             complement(5874..6425)
                     /locus_tag="PLK64_11170"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HPV80353.1"
                     /translation="MTTATKPPTPSPSVPVGLDWQLAIAQELIARGVSVAAYIPDSRL
                     NGVLAALGERDIRLRNLAREEECVAYAAGQRIAEARPVVLMQSSGLGNALNALGSLAV
                     PYRLGIPLIISMRGTLGEKNPSQVPMGRATTAWLGALGIQSFSLRQASEVGPVISGVL
                     DLAFDAGETAAVLLEPELGGGRA"
BASE COUNT         1026 a         2247 c         2208 g         1005 t
ORIGIN      
        1 ccgtctcact gggccccggc atgacgtgcg cccgaagcta actagttggt tgcgacgata
       61 cggcggcagg ctgacaaccg ccgtcggccg gcaccgacgg atcgccacac tcctcgcatc
      121 ggtcgcggag gccctcgatg catcgctttg cacctcgcat gcgttccgat gcgtaaagcg
      181 ctagaatagc tccatggctg acgtcaagaa gggaccgtct ccggtcgtat cgacagcctc
      241 gtttgaggca gaactgaatg ccctcctgct cagggccggg cacacggtgc ccggggcagt
      301 ggtcgccgca gcccttaagg ccgtggaacg tcgccgaagc cttgcgcgga ttggtcctga
      361 agcgaccacc tcaagccgaa cggagcagga cgagttcctc aaagcgagcg cgagcgtgga
      421 cgttgcgcca gatcagggcg cggcgatgct tcggctcgac ctatcccgcc gcgaggaggt
      481 catgctcacg acagcgcagg tggccaagga cctcgggaag gcgaagtcga cgatcactcg
      541 ccaggtcaac cggggtgacc tctacgcaat caagggtgat gggcagctgc ggttgccggc
      601 ctggcagttc gtgtccgggg ctgcagtccc cggactcgcc caggcactgg cctacgtccc
      661 cgagacctgg ggacctcgac ggcttcggct tttcatgaca acgcctgagg aagcactggg
      721 cgaccgcagc cccgttcagt ggctgctgga taacgagccc ccacaggtgg tcgcccaact
      781 gatggccgac gagagccgcg aatgacgccc aagccccaac cgaagacccc acgaaagccg
      841 ccgaaaccgt taacccgccg atccagcgac ctcgtcgggg tccggcccac gctctggcgc
      901 attggggcga ccatcgggga ctacgtgttg gagtggaacg aagcccgaga cttcgggccc
      961 ttgcggacga tgcggtggga cccgcaccac ccaccccaat ccgtacaacc ggaccgcgcc
     1021 gtcatctacg cttcaccgtc accgatcacc gccgtggctg aaaagttcca gaagtaccgc
     1081 accatcgacc ccttcaccgc agccccgtgc ctctacgggt ggtcggcaac acggcagttg
     1141 accctcctgg acctgcgggg caactggccg atcaacaaca agggatccgc gtcgatgccg
     1201 atgggaccga ggaacgtttg ccgggcttgg tccgccgcga tcctcgacac ctggcccgag
     1261 ctcgacgggc tcctgacacg atccacgatg gacggccgcg acacttacgt cctttttcct
     1321 ccggcgcgca cctcgatgcc cgacgaacct cgccacgcgc gactgctgac cgacccggcc
     1381 gtctactgct acctcgatct gtgggccgac gagatcaact acgacattgc cgacgcaccc
     1441 tggccacctg cctgagggga ctgccaaaac gggctggccg ctgcgccatc acctacttcg
     1501 cggtgatgcc gccgtcgacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     1621 ccagcgccag cgcgtaggag cgcaccagcg cgatgatccc gcccttggtg aacgaataga
     1681 gcgggctgaa cggcgacccg accaagcccg acgtcgagct cgtgaagatc accgacgcac
     1741 cgtccgcagc cttgagcgca tcgagcaggt gccccgtcag gaagaagctt gcccgcgcgt
     1801 tgatctcgat gccggtgtcc caatcggcct ccgagagttg cattcccgcg gctccgggga
     1861 tcccgacgtt gttgtagagg atgtgcacct tgccgtggtc agcggtgatc ttggcgccga
     1921 gccgctccaa cgcgtcgaga tcgcggacgt cgacggccgc tgcctctgcg ctgccgcccg
     1981 ccgcgatgat cccctcgacg gtcgccgtcg ccgcggcctc gttgacgtcg acgacgatga
     2041 cgtgcgcgcc gtgctcggcg aacaactgcg ccgacgcctg ccccatcccg gacgccccgg
     2101 cggtgatgac ggcgatcttg tccttgagca acatggtggt ctcctcggtg gtcgggctgg
     2161 tcaggcagta ggtctagtca ggcagtgggt cgggtcaagc agcagtcggt tcacgcagaa
     2221 ggtcggcggg cgatcaagtc ggcgagcgcg taggcctgct tcgtggcgaa caccagtcgc
     2281 cgcggcgcgt aggcgtcgcc caggatgtgc aggtcggtat gccgtccggc caggtcgtcg
     2341 cagagagtcg agtcgctcac gctgccacac gcaaggacca cagagtcgac gtccttgatg
     2401 acgcgaggcc gcagcgagta gacgttacgg gtgtgcaccc ggccggcttc gatggccgtg
     2461 acctcctcca tcgaccgcaa ctccacgccc tgctcatcga gccgtcccaa gatggccccg
     2521 acgatgtagc gccagagcaa cggggcgggg gtcgcggtcg cgaagacgac ggtcacctca
     2581 tggccacgcc cactgaggaa gtccgcgacc gacaacggcg cgacgtgatc gtcctgcgag
     2641 acgacgagca cccgtcgccc tggaatcgcc ctctccgcca acacatcccg cacgtccaac
     2701 acgtgtggca ggtcgtcacc gaggatgcct gggcgacgcg gggtggcacc ggtggcgatg
     2761 gccacggtgt cggcgccaac cgccaacaca tcgtcggccg tggcccggcg acccagggac
     2821 acctccacac cggcggtcgc caggcgcgcg acctgccagt cgaggtacgc ggcatacatg
     2881 tcgtggcggg gggcaagcgt cgcatagtgg agcgctcccc cgagacggtc ctgcgcctcc
     2941 cacaactgca cccggtgacc tgactcggcg agcagtgcgg acagttccat tccggccggg
     3001 ccgcctccga cgacgagcac ttcacggggc gagacggcct gcggcagtgg accttccact
     3061 tcgtggctgg tcgcggggtt gacggcgcac gcgaagccga gcccctccac ataccgccgg
     3121 ctgatgcact cgttgccacc aacgcagggg cggatctcgg cgacccgccc ctcgcgggcc
     3181 ttgaccagca actgcccgtc ggcgatgtgc gcccgagcca tccccacgac atcggcctga
     3241 ccagcggcga ggatccgctc agccacctcg ggcgcgttga tccggcccgt gtgcacgacc
     3301 ggcagcgaca cggcagcttt gagggccccg gccaactcgc cgaaagcacc cgcgtcgatg
     3361 taggtcggct ggatatagga cgggttgccc caggggctgc cgacgacgcc atggacatag
     3421 tcgatgaggc cggtcgactc gaagtactgc gcgagctcga tgccgccggc gaccccgaag
     3481 ccctcgggcg cctcctggcg gatgttgagc cggatgccga cggtcatcga ggttccgacg
     3541 acgtcgcgga ccgcctgcaa gacctcggtc gcgaagcgac accgattctc gagcgagccg
     3601 ccatatcggt cggttcggtg gttcgtatac ggcgacagga accactcgtg caggtcgtcg
     3661 tggttgaggt gcaactcgaa tccgtcggcg cggccgagca tcgacagccg agccgactcg
     3721 gcatactccc tgacgaactc ggcgatgtcg gcgtgggtca tgacgtgcgg gaccaggttg
     3781 atgaccgggg cgctcaggcg cgcggagggg gcgtgcggca tacccccttg gtggacgcac
     3841 tgcacgctga ccgtcgcgcc gagcgcgtgg caggcatcgg agaaggcctg gacccgctcg
     3901 acgaagaacg gcaggcggaa gaagccggtc gtggccgcac cgacgccggt ggggtcgaag
     3961 ccggggatga ggtggttgcg gatgtgggtg gacagcgagg cgatccaggc gactcccgag
     4021 cgagcgcgct cctcgaagta ggcgatgttg cgcgctgcct cgtcgtcggt gccgtagata
     4081 ttgccgatcg cggaggcgtg cggcggcagc atgacgcggt tgcgcaaccg catcgttccc
     4141 acggtgatgg gagcgaacaa gtgggggaag tgctgcccgg cgggctcgat cgtcatccgt
     4201 cgtctcccta ggtccgtgca tatgataacc atgaatcgcc caccggagcc agggtcggcg
     4261 gcgtcccaac cggggcggac ggggataggg tcgagcggtt gacctgttga tcccgaggtt
     4321 tagccccgac agttagcctc gacggcccgg acgaaggagc cctgccatgc ccgcgcggca
     4381 gacctccggc ggatcgctgc tcctggtcat ggccgcggtg gtctcggtgc agttcggcgg
     4441 ggccgttgcc gccacgctcg tgcccgtcat cggcgccgcc gggtcggtcg tgctccggct
     4501 gggcatcggc gcagtcgtcc tgctggcagt cgcgcgcccg accctacgcg ggcactcagc
     4561 ctcggcctgg cgcaccgttg tgctcttcgg cctcgtcctc ggcgcgatga actggtcgtt
     4621 ctacgcctcc ctcgaccgcc tgcacatcgg agtggcggtg acgatcgagt tcatcgggcc
     4681 gctcaccttg gccgccgtcc agtcccgccg ggtccgcgac ctgctcgccg tcgcggccgc
     4741 ggcggccggc atcctgctca tctcccaagc cctcaccgtg ccctggggcg acctggacct
     4801 catcgggatc ggcctggccc tgctggccgg caccttctgg gccggctaca tcgtgctgtc
     4861 ccagcggacc ggcgccgcgt tccccggact caacgggctg gccctggcga tgatcgtcgc
     4921 gctcgccctc gtgctgccct tcggaatctg gtccgtgccg ctgtggactg gcgacgtcct
     4981 cctcaaaggc gccgccgtcg cgatcctctc cagtgtcctg ccctattcgc tggagttgct
     5041 cgccctgcgt caactcagcc agcgagtctt cggcatcctg ctctccctcg agcccgccgt
     5101 cgccgccctc gccggcctca tcgtgctcgg acagcggctc accccaaccc aattggtcgg
     5161 catggccctc gtcgtggtgg cgagcgggct ggtcatgggt gcgggagggc ggcatacccc
     5221 cgcggatggg gcggccacct aagcgtcgcg cgcgaccgct tcggccgaac ggcatacctc
     5281 agtcccgcga agtcctcagg ctggagccgg cgcctgagcc agggacgcgc gcaggttgcg
     5341 cacggtggca gcggggtcga tgaagggcga cggaccgggc caagtgcgct cggtgatcgt
     5401 cgcctcgatg acgagcgggg cgccgggcgc gtccggcgag gtgggttcgg cgcgaagggt
     5461 tgcggcgagt tcggtcgtcg aactcaccgt aaccccacgc gtctcggcca gggcgttggc
     5521 caccgcggcg acgtgcagag gcgccgggat cggctgtccc ccggtgatgg agtaggcccc
     5581 atcggccaga accacgacgg tcaggttgct cggccggtat gccgcgacgc tccacagcgc
     5641 gctggccccc atgaggaact ccccgtctcc caggaccgcg acgacctggc ggtcaggcac
     5701 ggcctcggct agcccgaggg cgcccgccag tgccgagccc atggcggcgc cgaagtagaa
     5761 gtgtgggccg tcggaggtca ccaggcggac cgaggaggtc gcggtgccga gggaggacac
     5821 gaggatcgcg tcgggccggc actgcatgat cgtgcgggtc acatcgttga cgttcatgcc
     5881 cgtccacctc ccagctcggg ctcgaggagc actgcggccg tctcgccggc gtcgaaggcg
     5941 agatcgagca cgccggagat caccgggccg acctcggatg cctgccggag cgagaaactc
     6001 tggatcccga gggcgccgag ccacgccgtt gtcgcccggc ccatcggcac ctgcgagggg
     6061 ttcttctcgc ccagggtgcc gcgcatcgag atgatgagcg ggatgcctag gcggtagggc
     6121 acggccagcg acccgagggc gttcaaggca ttcccgaggc cggagctctg catgaggacg
     6181 acgggacggg cctcggcgat ccgttgcccg gcggcatacg cgacgcactc ctcctcgcgc
     6241 gcgaggttgc gcagccggat gtcccgctcc ccgagggccg ccaacacccc gttgagccgg
     6301 gagtccggga tgtatgccgc gacgctcacc cctcgcgcga tgagctcctg cgcgatcgct
     6361 agttgccagt ccaggccgac cggcacgctt gggctggggg tcggcggctt ggttgctgtg
     6421 gtcatgacgc tccgatcgcg ctccaccctg cggctgtcgg ggtcaagccg gggcggggcc
     6481 agtctgccca gaggctcgcg gtcgcgtcaa gttttgttca acaatcttgg ggagggcccg
     6541 cactcctgag taggccccga ggatgtcggc ctccgaatcc gtcagg
//
LOCUS       DAOMZY010000197         6577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_10974_length_6577_cov_6.099816, whole genome shotgun sequence.
ACCESSION   DAOMZY010000197 DAOMZY010000000
VERSION     DAOMZY010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6577
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10974_length_6577_cov_6.099816"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PLK64_11175"
     CDS             <1..272
                     /locus_tag="PLK64_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018778099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HPV80354.1"
                     /translation="RRAHASAPHERFLRRPYSYDDAPDAGSTSSSGLVFVAYQADPVR
                     QFVPVQARLAELDLLNLWTTPIGSAVFAVLPGAQEGEVLGQALFT"
     gene            complement(539..1027)
                     /locus_tag="PLK64_11180"
     CDS             complement(539..1027)
                     /locus_tag="PLK64_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80355.1"
                     /translation="MLKITLTDRDGDKPVASLGQRALFDRMAGDWVLSPERAKRERFA
                     YAVHDGKVVMAIEVLGVAIAGQSASPSTDDRYRLVGKPLKIGHPVYDAYVGKHAEGTR
                     NPIRYEDSTFDMGACRCGCGAETKSLFLPGHDQRAIHSRIAQVGSVAAFLDWFDETWS
                     AD"
     gene            complement(1242..2444)
                     /locus_tag="PLK64_11185"
     CDS             complement(1242..2444)
                     /locus_tag="PLK64_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80356.1"
                     /translation="MGDEIELISDGEGVVIAGEAAAVERFLAEHDWLGAALEYPPGSL
                     STWLRTAGDLAISAGQVAEHSTRYLKLTPESAQKLKDAGGLMPTKKDGISYAMLGKPG
                     DVSSWLQVEDGAAALLTNPAVLAGLGGVMSQFAQQAEAQELKALLVRMGEKLDDLRRA
                     QRDEVLAKLDAAAEAIKEAMTIRAHGGDPETLWDKVQGESSAIHEVQRYTLRALGALA
                     DKVEGKTKTGELKKVAREIEGDIGLQLSILARCFELQDEFRVVELDHVHATAPKTLAG
                     HRQGVEAARQQRRDDVLERTSRIMGRLDAAGEVAAANVLLHARSARSIVGSLNRTAET
                     IADFHAPLGVTLDREEVLLKAWTEALRDREQLKTAGIEVGQKASMAGTAAIGIGVMAV
                     AASKSREK"
     gene            complement(2643..3437)
                     /locus_tag="PLK64_11190"
     CDS             complement(2643..3437)
                     /locus_tag="PLK64_11190"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPV80357.1"
                     /translation="MALAIPVGTVLTNMTTGDNYRLEATIGQGGFGTIFAASPADGRP
                     LHDGVPFCVKVGLDADVWHGEAFYAGLLKGSTSVVRQLDAFPTFIPVRGGQRLGFAIV
                     MEYIPGGSVADHFGLGAEPWGEEQVLRKTKVLLRTLDLLHERGVSHRDITPMNVFVGN
                     RKALKLGDFGIAKTGLIRSGVRADVANWAFAPNDLQAFWRPADDVFQVGLLMATLASG
                     ELQDNTMGKVAVNRFTTKGPLRDAIKAAISVKSQRPQSAAELVRLL"
     gene            complement(3625..4923)
                     /locus_tag="PLK64_11195"
     CDS             complement(3625..4923)
                     /locus_tag="PLK64_11195"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HPV80358.1"
                     /translation="MRATTAFNKMLAIPGANVAAVTFTPEGIVVDLARRARRVRCPCG
                     WSTRAVYDRSARSWRGLDLGATRLVLRAEIRRLSCRRCGRVRTEDVPWARPGARFTRD
                     FEDVVAWLAQRMDKTAITRLLRCSWEAVANIVVRVVADQLDDTRLDEVYRIGVDEVSY
                     RKGHRYLTVVADHDRDGAVIWAGEGKNAATLNQFYDELGEQRCAQLQAVSMDLGAAFK
                     KATDAKAPQARQCVDPFHLIALANEAINKARRWAWNLERDKAHAAAQATGPHRGGRPP
                     AGSPPAPRNQARWVKNTRWALLKDPDALKPSQLEVLHELRRSGSVLYRCWQLKEGLRD
                     LYRLADPTDAPTHLDWWLAWACRSRIPAFLTLSKTVRANRDRILAAIELALSNSKLEG
                     LNSKIRLINHRGYGHHSAAALIANIYLCCGGLTIELPTGR"
     gene            complement(5145..>6577)
                     /locus_tag="PLK64_11200"
     CDS             complement(5145..>6577)
                     /locus_tag="PLK64_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HPV80359.1"
                     /translation="VVTYKTEYAQSLIDDFSVKEKAPHIAISVDMLDTGIDVPEVVNL
                     VFFKLVRSVSKFWQMIGRGTRLCEDLYGPGDHKANFYVFDFCMNLEYFNQPGAGSEGS
                     VQKSLGQRLFEARLGLVAALDHADLADEVGALRDDTAKQLHKVVVGMNLDNFVVRPER
                     EWVQRYAEWEPWTSLSAQAAGDIATHLAGLPSAVRDDDEDAKRFDLVILKIQLAKLDG
                     DELTADRLRQQVQQITSSLLNQSAIPAIAEQQALLAEVSEDEWWVDVTLPMLELARRR
                     IRGLVRFLEKTKRPIVYTNFEDEIGDGSIVTLPGVAVGTNWERFKAKARAYLKDHEDH
                     VALQKLRRNKQMTDTDLSALEEMLVASGAGGAEDISRAREESQGLGVFIRSLVGLDRE
                     AATDAFSEFLTDSRFSVAEIRFIQLIVEHLTANGVLEAKRLYEPPFTDHSPTGPDFLF
                     NDSDVDRLVVVLDQVRARAVVTQTVA"
BASE COUNT         1075 a         2158 c         2137 g         1207 t
ORIGIN      
        1 tccgccgggc gcacgccagc gccccgcacg agcggttcct ccgtcggccg tactcgtatg
       61 acgatgcgcc ggatgccggg tcgacgagtt cgtcgggtct ggtgttcgtg gcgtaccagg
      121 ccgaccccgt gcgccagttc gtgcccgtgc aggcccgcct cgccgaactc gacctgctca
      181 acctctggac caccccgatc gggtcagcgg tgttcgccgt gctgcccggt gctcaggagg
      241 gtgaggtgct gggccaggcc ctgttcacgt gacacggcgc ggaggcccac ccccgcgggc
      301 tgtgctgcca cgacatcggg gtcatgaccc cgatgtcgtg caacccaccc ggccggaaaa
      361 cggcgaagga ccccccgaca aagcgttggg gggtccttcg tcgtttggtg gctggtcaac
      421 gaaagtcgct gaccagcgca atcgcggatg ccacgggagg gttggcacgt cagcttcggt
      481 gcggttggca caggtgggtg gacggctgac gggtggcgtg ccgagacgcg ccgttcaatc
      541 agtccgcaga ccaggtttcg tcgaaccagt cgaggaaggc ggcgacggat cccacctggg
      601 cgatacgcga atggattgcc cgctggtcgt gccccggcag gaacagcgac ttcgtctcag
      661 ccccgcagcc acaccggcaa gcgcccatgt cgaaggtgga gtcctcatac cgaatcggat
      721 tccgagtgcc ttcggcgtgc ttcccaacgt aggcatcgta gacggggtgg ccgatcttca
      781 gcggcttacc cacgaggcgg tagcggtcgt ccgtgctcgg gctcgcggac tgcccggcga
      841 ttgcaacgcc gaggacttcg atggccatga ccaccttccc gtcgtggacg gcataggcaa
      901 accgctcgcg tttcgcccgc tccgggctga ggacccagtc gccagccata cggtcaaaga
      961 gggccctttg gcccagggac gccacgggct tgtcgccgtc gcggtcagtg agggtgatct
     1021 tcagcattga cctctccgta tacatttggt aaccgggagt atgaagtatt gcatgtagag
     1081 acctatactt gctactcccg gtgaccagga gtaaatcagt cgctcaatca ggccccgccc
     1141 gtcttgaacg tctggcaacg cccccacggg agccgcaccg gtcagggctc gacgacgccc
     1201 cactcatcca cagacgtgct cgtcggtgat gccgccggcg cctacttctc ccggctcttg
     1261 gaggcagcta cggccatgac accgatgccg atggcggcag tgccggccat cgatgccttc
     1321 tgtcctacct cgatgcctgc ggtcttgagc tgctcccgat ccctcaatgc ctcggtccag
     1381 gccttgagta gaacttcttc ccggtcgagg gtgactccca gcggcgcgtg gaagtcagcg
     1441 atcgtttccg ccgttcgatt gagcgagccg acgatggagc gggccgatcg ggcgtgcagg
     1501 aggacatttg ccgcggcgac ctcccccgcc gcgtcgaggc gccccatgat ccgactggtt
     1561 cgctcgagta cgtcgtcgcg gcgctgctgc cgggctgcct cgacaccttg acggtgtccc
     1621 gcgagcgtct tcggagccgt cgcgtggaca tggtccaact cgacgacgcg gaactcgtcc
     1681 tgcaactcga agcaccgggc aaggatcgac agctgtagac cgatgtcacc ctcgatctct
     1741 cgtgccacct tcttgagctc accggtcttg gtcttgccct ccaccttgtc cgccagcgct
     1801 ccgagcgctc ggagggtgta tcgctgcacc tcgtgaatgg cactcgactc gccttgcacc
     1861 ttgtcccata aggtctccgg atccccgcca tgcgctcgta tcgtcatggc ctccttgatc
     1921 gcttcagccg ccgcgtccaa cttggcaagc acttcgtcgc gctgggcccg gcgcaggtcg
     1981 tcgagcttct cgcccatccg cacgagcagc gccttgagct cctgagcctc agcctgctgg
     2041 gcaaactggc tcatcactcc accgagtccg gccagaaccg cggggttggt cagcagtgca
     2101 gcggcgccgt cctccacctg aagccacgag ctgacgtctc ccggcttgcc gagcatggcg
     2161 tagctgatgc cgtccttctt cgtcggcatg aggccacctg catccttgag cttctgcgcg
     2221 gactccggcg tcagcttcag atagcgagtc gagtgctcgg ccacttgccc agcgctgatt
     2281 gcaaggtcgc ccgcggtgcg cagccacgtg gacagactgc cgggggggta ctcaagcgcc
     2341 gcgccgagcc agtcgtgctc tgccaggaac cgctcgaccg ccgcggcctc tccggcaatg
     2401 accacgccct cgccgtcact gatcaactcg atctcgtcgc ccattcgcac ccctcaacgt
     2461 ctctcggtcc acaccgtagt cggccgggtg agctacaccg gcctcggacg gcggactcac
     2521 ccgacgcgcc gggatgatgc gagccggcgc cttgggctcc cgatggagaa cgagcccgag
     2581 cttccgtcca gccgtgaggt gcccgcgccg acgtagcgaa ggtgtgagtc aagtcccggc
     2641 tgctacagga gtctgacgag ttcagccgcg ctctgggggc gctgagactt caccgagatc
     2701 gccgccttga tcgcgtcacg cagcggaccc ttggtcgtga accggttcac ggcgaccttg
     2761 cccatcgtgt tgtcctgcag ttctccggat gcaagggtgg ccatgagaag cccgacctga
     2821 aacacgtcat ccgcaggccg ccagaaggcc tggagatcgt tgggggcgaa ggcccagttt
     2881 gctacgtcgg cgcgaacgcc cgaacggatc agcccagtct tcgcgatgcc gaagtcgccc
     2941 aacttgagag cctttcggtt tccgacgaac acgttcatgg gggtgatgtc tcggtggctc
     3001 acacccctct catgtaacag atcgagggtc ctcagcagca ccttggtctt cctgaggact
     3061 tgctcctcgc cccatggctc agcgccgagc ccgaagtggt cggcgacgct gccgccgggg
     3121 atgtactcca tgacgatcgc gaaacctaga cgctgcccac cgcgcacggg aatgaaggtt
     3181 gggaaggcgt cgagctgtcg aactacgctg gtggagccct tcagaagtcc ggcgtagaag
     3241 gcctcgccat gccagacatc tgcatctagg cccaccttca cgcagaaggg gacgccgtcg
     3301 tgaaggggac ggccgtcggc cggcgatgcg gcgaaaatcg tgccgaatcc tccctgtccg
     3361 attgtcgctt cgagtctgta gttgtcgccg gtggtcatgt tggtgagcac ggtgccgact
     3421 ggaatagcca aggccatgat cattccctcc gtagtagtcc cgatgcgtga agcatgctgc
     3481 ccgcaatgct gccgatctcc gatgactcga gctatgacgt cacgacggcc cttcaaggcc
     3541 atggacctgt cggactggac agcaagcccg gcggatccgt ggcgctttcc cgtccctcct
     3601 gtcggcgatc ataagaggtt ctcctcacct tcctgtgggt agttcgatgg tgagtccgcc
     3661 gcagcagagg tagatgttgg cgatgagggc ggcggcgctg tggtggccgt agccgcggtg
     3721 gttgatgagc cggatcttgg agttcagtcc ttcgagtttg gagttggaca gtgcgagttc
     3781 gatggcggcg aggatcctgt cgcggttggc tcgcacggtc ttggagaggg tgaggaaggc
     3841 agggatgcgg ctgcggcagg cccaggccag ccaccagtcc aggtgggtgg gtgcgtcggt
     3901 ggggtcggcg aggcggtaga ggtcgcgtag tccttccttg agttgccagc agcggtagag
     3961 caccgagccg ctgcggcgca gttcgtgcag gacctccagc tgggagggtt tgagggcgtc
     4021 ggggtccttc agcagcgccc atcgtgtgtt cttgacccag cgggcttggt tgcgcggcgc
     4081 cggtggactt cccgcgggtg gacgtccacc tcggtggggg ccggtggcct gggcggcggc
     4141 gtgggctttg tcccgttcca ggttccacgc ccagcggcgg gccttgttga tcgcctcatt
     4201 cgccagagcg atcaggtgga aggggtcaac acactgccgg gcctggggcg ctttggcgtc
     4261 ggtggccttc ttgaacgcgg ccccgagatc cattgacacg gcctgcagtt gggcgcagcg
     4321 ttgctcgccg agttcgtcgt agaactggtt cagggtggca gcgttcttgc cctcgccggc
     4381 ccagatcacc gccccgtcgc ggtcgtggtc tgcgaccacg gtcaggtacc ggtgtccctt
     4441 gcggtaggag acctcgtcca ctccgatccg gtagacctcg tccaggcggg tgtcgtcgag
     4501 ctggtcggcc acgacgcgga ccacgatgtt ggccaccgcc tcccaggaac agcgcagcag
     4561 ccgcgtgatc gcggtcttgt ccatccgctg agccagccag gccacgacgt cttcgaagtc
     4621 gcgggtgaac cgggcacccg ggcgagccca cggcacgtcc tcggtgcgca cccggccgca
     4681 gcggcgacag ctcaagcggc gaatctccgc ccgcaagacc agcctggtgg cccccaggtc
     4741 cagaccacgc cacgaccggg ccgagcggtc gtacacggcc cgggttgacc agccacatgg
     4801 gcatcgcacc cgccgggcac ggcgggccag atcgaccacg atcccctcgg gggtgaaggt
     4861 caccgcggca acgttggcac cgggaatcgc gagcatcttg ttgaatgcag tcgtggcgcg
     4921 cacgcggccg ctcctgtcct gagttcggtt ggcttggcag ctccgaacct agacagccga
     4981 cgcgtgcgcg tcacccaaca agcacccacc gctcccgata ccccacaccc ccaaccgaat
     5041 cagccgcgat cagggcccct cccgacccac aggaaggtga ggagtccctc ataagagggg
     5101 ttagatttcg ttaagaacca gacactctgt gcgggatgcc accgtcacgc gaccgtctgg
     5161 gtaacgacgg cgcgggcgcg gacctggtcg aggacgacca ccagccgatc cacatcgctg
     5221 tcgttgaaga ggaagtccgg tcccgtcggg gaatggtcgg tgaagggcgg ttcgtagagg
     5281 cgcttggcct ccagcacccc gttggccgtc aggtgctcga cgatcagctg gatgaaccgg
     5341 atctccgcga cgctgaaccg tgagtcggtg aggaactcgg agaaggcatc ggtcgctgct
     5401 tcacgatcca ggccgacgag cgagcggatg aagacgccga gcccttggga ctcctcgcgc
     5461 gctcggctga tgtcctcggc tccgccggcg ccggacgcaa caagcatctc ttccaaggcg
     5521 gacagatcgg tgtcggtcat ctgcttgttc cgacgcagtt tctgcaaggc gacgtggtcc
     5581 tcgtggtcct tcaggtaggc acgcgccttg gccttgaacc gttcccagtt ggtgccgacg
     5641 gcaactccgg gcagggtgac gatcgacccg tcgccgatct catcctcgaa gttggtgtag
     5701 acgatcgggc gcttggtctt ctcgaggaac ctgaccaggc cacgaatccg tcggcgcgct
     5761 aactcgagca tcggcagggt gacgtccacc caccactcat cctcggagac ctccgccagc
     5821 agcgcttgct gttcggcgat ggcagggatg gcgctttggt tgagcagtga tgacgtgatc
     5881 tgttggacct gctggcgcag gcgatccgcg gtcaactcat cgccgtcgag cttggcgagc
     5941 tggattttga ggatcaccag gtcgaaccgc ttggcgtcct cgtcgtcgtc ccgtacggca
     6001 gacgggagac cagccaggtg agtggcgatg tcgccggccg cctgcgccga tagagacgtc
     6061 catggctccc actccgcgta cctctggacc cattcccgct cgggacgcac cacgaagttg
     6121 tcgaggttca taccgacaac gaccttgtgc aactgcttgg ccgtgtcatc acgcagcgct
     6181 ccgacctcgt cggcgaggtc agcgtgatcg agtgccgcta cgagtcctag gcgcgcctcg
     6241 aagagtcgct gcccgagcga cttctgcacc gacccctcgg acccggcacc gggctggttg
     6301 aagtactcga ggttcatgca gaagtcgaag acgtagaaat tggccttgtg gtcaccgggc
     6361 ccgtagaggt cctcgcagag ccgggtgcca cggccgatca tctgccagaa cttcgacacc
     6421 gaccgcacga gcttgaagaa gacgaggttg acgacctcgg gcacgtcgat gccggtgtcg
     6481 agcatgtcga cgctaatcgc gatgtgcggc gccttctcct tcacggagaa gtcgtcgatg
     6541 aggctctggg cgtactcggt cttgtacgtc acgaccc
//
LOCUS       DAOMZY010000198         6551 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_11020_length_6551_cov_5.113762, whole genome shotgun sequence.
ACCESSION   DAOMZY010000198 DAOMZY010000000
VERSION     DAOMZY010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6551
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11020_length_6551_cov_5.113762"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2260)
                     /locus_tag="PLK64_11205"
     CDS             complement(<1..2260)
                     /locus_tag="PLK64_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014923536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPV80360.1"
                     /translation="MTVAFKDAAGVIDEAVVTRDREASLRLVASSSIRAFDGDGESWR
                     LAAEALRIRYAALFDPMLVVTSSDVEPLPHQITAVYGEMLPRTPLRFLLADDPGAGKT
                     IMCGLYIKELMLRGDLDRCLVVAPGSLVNQWMDELFDKFGLRFELLTKQLVEATPEGL
                     FEQHPLLIARMDQLSRSQELIDLLKSSDWDLVVVDEAHRMSASYFGGELKTTKRYQLG
                     QVLGGVTRHFLLMTATPHAGQQEGFQLFLALLDGDRFEGQFRDGVHTVDPGDLMRRMV
                     KEELLTMEGKPLFPERRAYTVPYDLSDDEQDLYEAVTLYVREEMNRADRFAAEGDRVR
                     RNTVGFALTVLQRRLASSPEAILRSLERRRARLERRRRELAGDAHLGLSFTTKMAQLL
                     GRKDDALDLEEAFEDLDGAEVEELEEDVVDAATAARTLAELDLEIAALGDLVHVASRV
                     RHSGTDRKWSELRDILDREEITEDRDGNPRKIIVFTEHRDTLNYLVDRIVTYLGRPEI
                     VVSIHGGHTREERRIAQQRFTQDPETRVLVATDAAGEGLNLQRAHLMVNYDLPWNPNR
                     IEQRFGRIHRIGQTEVCHLWNLVAKDTREGEVFIRLLDKIEEQRRAYGGDKVFDVLGD
                     AFENNPLRRLLVQAIRYGDQPEVRARLDEIIDATVGSGLDALLAERALHRQTLAATDV
                     EAIRERVEQAQARRLQPHFLEAFFRAAFADVGGRMAPREAGRFEVTNVPAALRDRDRI
                     MGVGAVVLARYER"
     gene            2587..2973
                     /locus_tag="PLK64_11210"
     CDS             2587..2973
                     /locus_tag="PLK64_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPV80361.1"
                     /translation="MSTTTNWPGDWLRGVLEMAVLRVLANEPSSYGYAISVALDRNGL
                     GTVKGGTLYPLLARLEQAAHVETRWEAGDGGPGRKYYTITDSGRSHLESAAASWCAFA
                     ETTATFLRAEAPGIPAPSPATTVPAG"
     gene            3000..3695
                     /locus_tag="PLK64_11215"
     CDS             3000..3695
                     /locus_tag="PLK64_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80362.1"
                     /translation="MLDPTKTTLPKQWASEFAMALRSRDVTGAAIGDALREAEAYCAD
                     SGQAPLEAFGPAAAYADSLTDLPTAAAHTSWAARIAPSALGLAGLTLALPTFDAWRAG
                     RSVQLPLGGLVTLIVIAATVTVFLVWPRTFRRAVPFLILVSLGMWGSVSATALLQQPV
                     ATLPTVPSALVAAGALIGSGIWQMRTLDMDVIVDPLGPTPRPGRGFLLLTAWLLPIGT
                     LVAMGIAMFAPVR"
     gene            complement(3710..5209)
                     /locus_tag="PLK64_11220"
     CDS             complement(3710..5209)
                     /locus_tag="PLK64_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPV80363.1"
                     /translation="MSPTPVPDPDLLPRLRADLTAAAYTVDGVEHTLGPVAAGALHRE
                     QRLPADLATRHRPEPAAVLVRLFALGFPVAWDDADRALPTLRADGAVQLGLARAEGAD
                     LRSTCDLRPYADESHQWWVASDLSEIATGRPLAETHVLGIGNASTTLAEWTIRRQARR
                     ALDLGVGSAVQSLHLATHVEGIVGTDVSERALAFARFNADLNDLAIELRPGSLLDPVA
                     GERFDLIVSNPPFVITPRSAGVPLYEYRDAGLVGDGVVEALVRRVGEHLEPGGVAQFL
                     GNWEIRRGADWRDRWHEWLDGTGLDAWVIQRDVQDPAQYAELWARDAGARSGAPNFDA
                     MYAAWLADFADRHIEGIGFGIITLQRPLGDREPWLDLVEVPGPVAPGVGHAVDAGLRA
                     RTAIAEGGTAYLLDTPWSAAPDVTEERHLLPGAEDPSVILARQGGGLRRTVQLDTVAA
                     GLIGVCDGSLTPRVALAAIASLLELDDAHVTEVAVPTLRALVADGFLVA"
     gene            complement(5314..5658)
                     /locus_tag="PLK64_11225"
     CDS             complement(5314..5658)
                     /locus_tag="PLK64_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012630959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPV80364.1"
                     /translation="MTTTRLELAAPCCALGAPPVSPADAEAIAPMFKALGDPVRLRLV
                     SLIAAQPEACVCDLVGAFPLSGPTISHHLKVLRAAGLVDCERRGTWVYYWVRPEALAR
                     LSSLLGRPISPA"
     gene            5731..>6551
                     /gene="arsM"
                     /locus_tag="PLK64_11230"
     CDS             5731..>6551
                     /gene="arsM"
                     /locus_tag="PLK64_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HPV80365.1"
                     /translation="MSQSSSPAGPHTSTAGLDTPAEQVRSVVQERYAAAARTTLDLLE
                     GTETAAGSCCAPSCCAEPAAADPITHTETAAAPCCGSSCCATPTGADPITRDLYEPGE
                     VPSAGALVASLGCGNPTLLADLQPGQSVLDLGSGGGLDVLLSARRVAPGGTAYGLDMT
                     PEMLELARRNQAEAGVENATFLLGTIEEVPLGDASVDVVISNCVINLSPDKDAVLREA
                     FRVLRPEGRFAVSDIVLLRPIPEHLTGIVGLWTGCVSGALLDSDYVAKLGAAGFV"
BASE COUNT         1086 a         2243 c         2196 g         1026 t
ORIGIN      
        1 cccgctcata cctggccagc acgacagccc caacacccat gatccggtca cgatctcgga
       61 gcgcagccgg gacattagtg acctcgaagc gtcctgcttc cctcggcgcc attcggccac
      121 ccacgtcggc gaaggccgcc ctgaagaacg cctcgagaaa gtgcggctgc aagcgacgag
      181 cctgtgcctg ttcgacccgc tcacggatgg cctccacatc ggttgctgcg agcgtctgcc
      241 tgtgcagggc gcgttcggcc aggagggcgt ccagcccaga gccaacggtg gcgtcgatga
      301 tctcgtccaa acgggcacga acttcgggtt ggtctccata ccggatggcc tgaaccagga
      361 gacgccgcaa ggggttgttc tcgaaggcat cccctagcac gtcgaagact ttgtcaccgc
      421 catatgcccg gcgctgctcc tcgatcttgt ccaagagcct gatgaagact tcgccctctc
      481 gggtgtcctt cgcgacgaga ttccacaggt ggcagacctc cgtctgcccg atccggtgaa
      541 tgcgaccgaa gcgctgttcg atgcgattgg ggttccaggg gaggtcatag ttgaccatga
      601 ggtgggcccg ctgcaggttg agcccttccc ctgccgcgtc ggtcgccacg agaactcggg
      661 tctcgggatc ttgggtgaac cgctgctggg cgatccgccg ctcctcgcgg gtgtgtccgc
      721 cgtggatcga taccacgatc tccggacggc cgagataagt gacgatccga tcgaccaagt
      781 agttgagggt gtccctgtgc tccgtgaaga cgatgatctt gcgggggttg ccatcgcgat
      841 cctcggttat ctcttcacgg tcgaggatgt cgcgcaactc ggaccacttg cgatccgttc
      901 ctgaatgacg gacccgcgag gccacgtgca cgaggtcacc aagggcagcg atttcaaggt
      961 caagttcggc caaggtccga gcggcggtag ccgcatcgac gacgtcctcc tccaactcct
     1021 cgacctctgc tccgtcgagg tcctcaaaag cctcctccag atccagggcg tcatccttgc
     1081 gtcccagcaa ctgagccatc ttggtggtga aggaaagccc aagatgcgcg tctccggcca
     1141 actctcggcg tcgacgctca agacgtgcgc gcctgcgctc caagctgcgc aggatggcct
     1201 cggggctgga cgccagccgc cgctgcaaga cggtcaacgc gaaaccaacc gtattccggc
     1261 gcacgcgatc gccctctgcc gcaaaacgat ccgcgcggtt catctcctcg cggacataga
     1321 gcgtgactgc ctcatacaag tcttgctcgt catcagacag gtcgtaggga acggtgtagg
     1381 cccgccgctc ggggaacagc ggcttcccct ccatggtcaa cagctcttcc ttgaccatgc
     1441 gccgcatcag gtcacccgga tcgactgtat gcacgccgtc acggaactga ccttcgaagc
     1501 ggtcgccatc cagcagagcc aggaagagtt ggaagccctc ctgctggccg gcatgcggag
     1561 ttgctgtcat gaggaggaag tgccgcgtga ccccgccgag cacctgaccc agctggtaac
     1621 gcttggtcgt cttcaactcg ccgccgaagt agctggccga catccggtgc gcctcgtcga
     1681 cgacgacgag atcccaatca gagctcttga ggaggtcgat caactcctga ctccgggaga
     1741 gctggtccat tcgcgcgatc agcagcgggt gctgctcgaa cagaccttcg ggagtggcct
     1801 cgacgagttg tttggtgagc agctcgaacc gcaggccgaa tttgtcgaac aactcgtcca
     1861 tccactgatt gaccaggctc cctggcgcaa cgacgaggca ccgatccaga tcgccgcgaa
     1921 gcatcagctc tttgatgtac agcccgcaca tgatggtctt gcccgcgcca gggtcatcgg
     1981 cgaggaggaa ccgcagcggc gtgcgcggca gcatctcgcc gtagaccgcg gtgatctggt
     2041 gcggcaacgg ctccacgtcg gaggaggtga ccacaagcat ggggtcgaac aaggcggcat
     2101 acctgattcg aagtgcctcg gcagcaagcc gccacgactc gccgtctccg tcgaatgccc
     2161 gaatcgagct gctcgccacc agccggagac ttgcctcgcg gtcgcgggtg accaccgctt
     2221 cgtcgatgac ccctgcggca tccttaaacg ccacggtcac gacattcccg ccatgccact
     2281 tgaccgacag gatgtccacg ggagcgtgaa ggcgtaaacc ttccactctc gctccgggcg
     2341 tcatcagctc caatcggaaa gcgtggacag cctcaagcgg agagttaccg gagttcgcgt
     2401 cgtcgaccac aggcataggc atagcacgcc cggccgacat aggccggacg agcgacgttc
     2461 ctttgccgcg gaagtgtcgg tgacagccct tgggccatgt cagcgctcgg cacgcgctca
     2521 gcaggacaac ttgatcgcct aggcaagtag atagacaaac tgactagatc ggcataccat
     2581 ctagtcatga gcacgacgac gaactggccc ggagactggc ttcggggcgt gctggagatg
     2641 gcggtccttc gggtgctggc caacgagccg tcgtcctacg gttacgccat ctctgtcgcg
     2701 ttggaccgca acgggttggg caccgtcaag ggcggcacgc tctatcccct gctcgcccgc
     2761 ctcgagcagg ccgcccacgt tgagacccgt tgggaggccg gcgacggcgg gccgggccgc
     2821 aagtactaca cgatcaccga ctccgggcgc tcccacctgg agtcagcagc cgcctcctgg
     2881 tgcgcgttcg ccgagaccac ggcgaccttc ctgcgcgcgg aggcacccgg catacctgca
     2941 ccctcccctg ccacaaccgt gccggcgggc tgatctcccc accccggaaa ggaaccacca
     3001 tgttggaccc cacgaagacg accctcccga agcagtgggc gagcgaattc gccatggcac
     3061 tgcgctcccg ggacgtcacc ggagcggcga tcggcgacgc cttgcgtgag gcggaggcct
     3121 actgcgcaga cagcggccag gcacctctgg aggcgttcgg tccggctgcg gcgtatgccg
     3181 attcgctcac cgacctgcca accgccgccg cgcacacgtc gtgggccgcg cggatcgccc
     3241 cgtcggcgct cggtctggcc ggcctcaccc ttgccctgcc gacgttcgac gcctggcgag
     3301 cggggcgcag cgtgcagttg ccgcttggcg gtctggtcac cctgatcgtc atcgcggcga
     3361 cggtgaccgt cttcctcgtc tggccgcgga ccttccgccg cgcggtgccg ttcctcatcc
     3421 tcgtgagcct gggcatgtgg ggctcggtgt cggccacggc gctgctccag cagccggtcg
     3481 cgaccctccc cactgtgccg agcgccctcg tcgccgccgg ggccctcatc gggtcgggca
     3541 tctggcagat gaggactctc gatatggacg tgatcgtcga tcccttggga ccgacgccgc
     3601 gccctggtcg cgggttcctg ctgctcacgg cctggctgtt gcccatcggc accctggtcg
     3661 ccatgggcat cgcgatgttc gcgccggtgc gctagatccg caggacgcct caggcgacga
     3721 ggaacccgtc ggcgaccagg gcccggaggg tgggaacggc gacctcggtg acgtgcgcgt
     3781 cgtcgagctc caacaaactg gcgatcgccg ccagcgccac ccgcggcgtc agtgagccgt
     3841 cgcagacgcc gatgagaccg gccgccacgg tgtcgagttg gacggtgcgg cgcaggccgc
     3901 cgccctgacg ggcgaggatc accgacgggt cctcggcacc cggcagcagg tgtcgctcct
     3961 cggtgacgtc cggcgccgcg ctccacggcg tgtccaacag gtatgccgtc ccgccttcgg
     4021 cgatcgcggt ccgggctcgc aagccggcgt cgaccgcatg gccaaccccc ggagccactg
     4081 gtcccgggac ctcgacgagg tcgagccacg gctcgcggtc gccgagcggc cgctgcaggg
     4141 tgatgatgcc gaagccgatg ccctcgatgt ggcggtcggc gaagtcggcc agccacgcgg
     4201 catacatcgc gtcgaaattc ggtgcgccgg aacgggcccc ggcgtcgcga gcccacaact
     4261 cggcatactg cgccgggtcc tgcacgtcac gctggatgac ccaagcgtcc aggccggtgc
     4321 cgtcgagcca ctcgtgccag cgatcgcgcc aatcggcgcc gcgacggatc tcccagttgc
     4381 cgaggaactg cgcgacgcca ccgggctcga ggtgctcccc cactcggcgg acgagcgcct
     4441 cgacgacccc gtcgccgacc aggcctgcgt cgcggtattc atagagcggc accccggccg
     4501 atcggggcgt gatgacgaag ggcgggttgc tcacgatcag gtcgaagcgt tctccggcaa
     4561 cgggatccag caaactgccg ggccgcagct cgatggccag gtcgttgaga tcggcgttga
     4621 accgggcgaa ggccagggcg cgttcggaga cgtcggtgcc gacgatgccc tcgacgtggg
     4681 tggccaggtg caacgactgg acggcgctgc caacccccag gtcgagcgcc cgacgcgcct
     4741 gccgccggat ggtccactca gcgagggtgg tcgaggcgtt cccgatcccc aagacgtggg
     4801 tctcggccag tggacgtccg gtcgcgatct cggagaggtc ggaggcgacc caccattggt
     4861 gggactcgtc ggcgtagggg cgcaggtcgc aggtcgagcg caggtcggcg ccctcagcgc
     4921 gggccagccc gagttggacg gccccgtcgg cccgcagcgt gggcagcgcg cggtcggcgt
     4981 cgtcccacgc gaccgggaag cccagggcga agagccgcac gagcaccgcg gcgggctcgg
     5041 ggcggtggcg ggtggccagg tcggccggaa ggcgctgctc gcggtgcaga gcgcccgcag
     5101 ccaccgggcc aagcgtgtgc tcgaccccgt ccaccgtgta tgccgcagca gtcaagtcgg
     5161 cgcgcaggcg cggcaggaga tcagggtcgg gcacaggcgt cgggctcacg gcgacaaggg
     5221 tatgccgccc cctgccgggt cgaggtattc ggccgttacc cggcgatggt ggaatacctc
     5281 gaatcagctg ggggtcggac tggcgaggcg gggtcaggct ggtgagatgg gacgcccgag
     5341 cagggaactg agccgggcca acgcctctgg cctcacccag taatagaccc aggtgccccg
     5401 acgttcgcag tcgaccaacc ctgcggcccg aaggaccttg aggtggtggg agatcgtcgg
     5461 cccggacaac gggaaggcgc ccacgagatc gcagacgcag gcctcgggtt gagcggcgat
     5521 gagcgacacg aggcgcagcc gcaccgggtc accgagggct ttgaacatcg gggcgatcgc
     5581 ctcggcgtcg gcgggcgaca cgggcggcgc accgagcgcg cagcaggggg ccgccaactc
     5641 caaccgggtc gttgtcatgg gcctatcttg acagatatcg aaacaagatg cagcatcgtg
     5701 tttcgacagt catcgaaaca aggagagacc atgagccagt catccagccc tgccggcccg
     5761 cacacctcga cggcgggcct cgacaccccg gcggagcaag tccgctcggt cgtccaggag
     5821 cgctatgcgg cggccgcccg cacgaccctc gacctactcg agggcaccga gaccgccgcc
     5881 ggatcctgtt gcgccccctc gtgctgcgcg gaacccgctg cggccgaccc gatcacgcac
     5941 accgagacgg ctgccgcgcc gtgctgtggg tcctcgtgct gcgcaacacc gaccggggcc
     6001 gacccgatca cgcgcgacct ctacgagccc ggcgaggtcc cgtcggccgg cgccctcgtc
     6061 gcctcgctcg gctgtggcaa cccgaccctg ttggccgacc tgcagcccgg gcagagcgtc
     6121 ctcgacctcg gctccggtgg cggactggat gtcctgctgt ccgcgcggcg agtggcaccc
     6181 ggcgggacgg catacgggct cgatatgacc ccggaaatgc tggaactggc ccgtcgcaac
     6241 caagccgagg cgggggtcga gaacgcgacg ttcctgctcg ggacgattga ggaggtgccg
     6301 ctgggcgacg cgtcggtcga cgtcgtcatc tccaactgcg tcatcaacct gtcgcccgac
     6361 aaggacgcgg tgctccgcga ggcgttccgc gtgctgcgac cggaggggcg gttcgcggtg
     6421 tcggacatcg tgctgctgcg gccgatcccg gagcacctca cgggcatcgt gggcctgtgg
     6481 accgggtgcg tgtcgggggc cctgctggac tccgactatg tggcgaaact cggcgcggcg
     6541 gggttcgttg a
//
LOCUS       DAOMZY010000199         6518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_11101_length_6518_cov_6.291196, whole genome shotgun sequence.
ACCESSION   DAOMZY010000199 DAOMZY010000000
VERSION     DAOMZY010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11101_length_6518_cov_6.291196"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PLK64_11235"
     CDS             <1..217
                     /locus_tag="PLK64_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013490972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80366.1"
                     /translation="GAAAGLTENAVGPRLARTSLLAAYRDGDRVTRTGVERARYDLEE
                     GRHKVSPPTPDEERPPLRFRARRPESR"
     gene            234..887
                     /locus_tag="PLK64_11240"
     CDS             234..887
                     /locus_tag="PLK64_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013490971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HPV80367.1"
                     /translation="MTDQNLTHLYFLLDRSGSMASIRSETEAGFNAFIAEQRTAPGRC
                     LVTLAQFDDHYEEVYAARPVNEVPPLHLAPRGTTALLDSIGRLVTTAGERLAALPEHD
                     RPGTVIVGIMTDGHENASHEWGFGAVRALIEQQTTIYSWDFLYMGADQDAIEVGAQLG
                     VSREQSLTYDRHRVGSAYASASGNISAMRAARAAGASPAAAREKLAFTEAQRADAEA"
     gene            918..1889
                     /locus_tag="PLK64_11245"
     CDS             918..1889
                     /locus_tag="PLK64_11245"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HPV80368.1"
                     /translation="MEPEDWTVEDLVRTRHTEGSRPGERSDPCRLALLIEGGSSRSAY
                     SSGMVVAIEDLGLTTVFDAVYGVSGGALNGAWLLCGRAAQVMDTWWNREVMRGVIAPR
                     RALRGRPVVDTDYLVNTVYVDLVPMDFAAILGNSITFHPIATDAESGLAVDLAPLIHD
                     ESTLRLALRSSTGIPLLAGPPVHLAGRRFMDGGLAEMVPVRTALAQGATHAVVLRTRR
                     PGEDLRPPGNMQFHIIRRYLKRHAAGAIPAWRNRYAVAQDEEKLLATHPRVRQIRPPS
                     HGPTVPGTSRGARRLKATVDLGRATARSTLTRWLANSDPVGSTHSRG"
     gene            1902..4133
                     /locus_tag="PLK64_11250"
     CDS             1902..4133
                     /locus_tag="PLK64_11250"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPV80369.1"
                     /translation="MVSPVTRRVGVLTSGGDAQGMNAAVRAVARAGIRFGAEVFAIHE
                     GYQGAVDGGDGISALGWEDVGGIQHRGGTVIGTARCAAFRERAGRKQAAYHLAAQGID
                     RLVVVGGDGSLTGANLLSTEWPQFLAELAADGVLPADIVATHPTLHIAGIVGSIDNDM
                     VGTDMTIGTDSAMHRIVEALDAISSTAASHQRTFVVEVMGRRCGYLALTAAIAGGADF
                     ALIPEAPPGDGWEEELCESLRRARERGRRESIVVVAEGARDRSGEPITADRICAVLEE
                     RLGEQARVTILGHVQRGGSPSAFDRSMATMMGYDAVDQVLTAGPNDGAQLVGVHRNRV
                     RRVPLMRAVQENQAVARMVDEGHYEQALAARGDSYADLFGLLRTLSHPRTTLPADPKR
                     IAVLHAGGLAPGMNAAVRAVTLLGSDAGHEILGVDGGFTGLCDGRLRTLTPRDVDGWA
                     GLGGAELGSRRGVLAPEQLYAVARAVDTHRIDALLVVGGLDGYESAQRIVRERGRYPS
                     LDIPVVCLPATIDNNLPGTELTVGADTALNAIVEAIDRIKQSALAAHRCFVIEVMGRE
                     CGYLTLMSALSAGAERAYLPEDDLTLTDLAADVRSMTQRFRDGHGLFLAIRAEGAHPY
                     YTTDVLARIFEAEADDLYDVRQAVLGHVQQGGNPSPFDRVLAARLAADAVAAVSAALG
                     AGRSDAVAIGLDNGEIARTPLRRVDDLVDWARRRPRDQWWLRLRRVMRDLAEPAVDTR
                     HTP"
     gene            4325..5887
                     /locus_tag="PLK64_11255"
     CDS             4325..5887
                     /locus_tag="PLK64_11255"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPV80370.1"
                     /translation="MSINAFAALGVPASLADLLAKAGIDTPTPIQAATLPDSLAGRDL
                     LGRGRTGSGKTYAFLLPILARLAPNGVAPRNARTPRAPRALIVVPTRELAVQIEAALT
                     PLARLAGLNSLTIFGGVGQSPQVRALRAGVDIVIACPGRLEDLIGQGECRLDRVEVTV
                     LDEADHMSDLGFLPAVRRLLELTPASGQRLLFSATLDGAVTVIVKRYLRNPVTHEADS
                     PESATPDVEHHVLQVDSEQRMPVLVDLTSGSRRSVVFTRTKHRAKTLARKLNALGVSS
                     VELHGNLAQNARTRNLAAFHSGEASTLVATDIAARGIHVDDVELVIHADPPSEFKAYV
                     HRSGRTARAGAKGTVITLMEREQAAEVRALVRAAGIRPTTTSVGLGHPLLSELVPGER
                     RLVPVSARPAETVAAPEGRGSGTGGGGRGARSGRSGSGGSASPSAGSGRGGAARSGGT
                     RGSAPRSGAPRAGGSAPGAPRSDAGRSGAPRSGSARSGSAGGQRSGAARAGSLSAARM
                     SEQSGGRRRSGR"
     gene            5963..6436
                     /locus_tag="PLK64_11260"
     CDS             5963..6436
                     /locus_tag="PLK64_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HPV80371.1"
                     /translation="MSQPPPVPTLLVVHHTTSPALESMLDAVLAGTRADGIDGVDVVV
                     RPALAATASDLLAAGAVLLGTPANIGYMSGALKHFFDQVYYPCLHATVGTAYGLYVHG
                     NLDTVGAARSVHAIATGLQWRRAADDVVVLGPPDAQALESCWNLGATLAASLMPQ"
BASE COUNT          910 a         2478 c         2149 g          981 t
ORIGIN      
        1 cggggccgcc gcggggctga ccgagaacgc cgtcggcccg cgcctcgcgc gcaccagcct
       61 gctcgcggca taccgggacg gcgatcgggt gacccgcacg ggcgtggagc gcgcgcgcta
      121 cgacctcgaa gaggggcgcc acaaggtgtc cccgcccacc ccggacgagg agcgaccgcc
      181 gctgcgattc cgcgcgcgtc gccccgaatc ccgttgatcc acaaggagat ccgatgaccg
      241 atcagaacct cacccacctc tacttcctgc tcgaccggtc cggctcgatg gcctcgatcc
      301 ggtccgagac ggaggctggc ttcaacgcgt tcatcgctga gcagcgcacc gcgccgggcc
      361 gttgcctggt gacccttgcg cagttcgatg accactacga ggaggtgtat gccgcgcgcc
      421 ccgtgaatga ggtcccgccc ctgcacctcg ccccccgcgg caccaccgcg ctgctcgact
      481 ccatcggccg ccttgtgacg actgcgggcg agcggctcgc cgcccttccc gagcacgacc
      541 gcccggggac ggtcatcgtc gggatcatga ccgacggtca cgagaatgct tctcacgaat
      601 ggggattcgg cgccgtccga gcactcatcg aacagcagac cacgatctac tcctgggact
      661 tcctctacat gggggcggac caggacgcga tcgaggttgg ggctcaactg ggtgtctcac
      721 gggagcagtc gctcacctac gatcgccatc gggtcggatc ggcatacgcg tcggcctcgg
      781 gcaacatctc ggccatgcgc gcagcccgcg ccgcgggcgc cagccccgcc gctgcccgcg
      841 agaagctcgc cttcaccgag gcccaacgcg ccgacgccga ggcctgaccg ccgccgcgat
      901 ccgcgtaggc tccaacggtg gagcctgagg actggacggt cgaggacctc gtgcggacgc
      961 ggcataccga gggcagccgc cctggggaac gaagcgatcc gtgccggctc gccctcctca
     1021 tcgaaggcgg cagttcccga tcagcctact cctccggcat ggtcgtcgcc attgaagacc
     1081 tcggtctcac aacggttttc gatgcggtct atggagtctc aggcggcgca ctcaacggag
     1141 cctggctgct ctgcggccgc gccgcccagg tcatggacac ctggtggaac cgcgaggtca
     1201 tgcgcggagt catcgcccca cgccgggcct tgcgcgggcg gccggtcgtt gacaccgact
     1261 atctcgtcaa caccgtctat gtcgatctcg tgccgatgga tttcgctgcc attcttggca
     1321 attcgatcac cttccacccg atcgccaccg acgcggagag cggcctggcc gtcgatctgg
     1381 cccccctcat ccacgacgag tcgaccctgc gcctggccct ccgatcctcg accggcattc
     1441 ccctgctcgc cggcccaccc gtgcacctcg cgggcaggcg gttcatggac ggtggcctgg
     1501 ccgagatggt ccccgtgcgc accgcactcg cccagggcgc gacccacgcc gtcgtcctgc
     1561 gcacgcggcg gccgggcgag gacctgcggc ccccgggcaa catgcagttc cacatcatcc
     1621 gtcgctacct caaacgccac gcagctggcg caatccccgc ctggcgcaac aggtatgccg
     1681 tcgcccagga cgaggagaag ctcctcgcca cccaccctcg ggtccgccag atccggccac
     1741 cctcgcacgg ccccaccgtg cccgggacgt cccgtggggc ccgacgactc aaggccacgg
     1801 tggacctggg ccgagcgacg gcccgctcca ccttgacccg ctggctcgcc aattccgacc
     1861 cggttggctc cactcactcc cgcgggtgaa ggatgggctc catggtttcg cctgtcacac
     1921 gacgcgtcgg ggtcctcacc agcggcggcg acgcccaggg catgaacgcc gcggtccgcg
     1981 ctgtcgcaag agccgggatc cgtttcggcg cagaggtttt cgcgatccac gagggctacc
     2041 agggggccgt tgacgggggc gacgggatct cggcactcgg ctgggaggat gtcggcggca
     2101 tacagcaccg cggtgggacg gtcatcggga cagcgcggtg cgcggccttc cgggagcgcg
     2161 cgggccgcaa gcaggcggca taccacctag cggcccaagg catcgaccgc ctggtcgtcg
     2221 tgggaggcga cggcagcctc accggcgcca acctcctgtc caccgagtgg ccgcagttcc
     2281 tcgccgaact cgccgcggac ggagtcttgc ccgccgacat cgtcgccacc cacccgaccc
     2341 tgcacatcgc cgggatcgtc ggctcgatcg acaacgacat ggtcggcacg gacatgacca
     2401 tcggcaccga cagcgccatg caccgcatcg tcgaggcgct cgatgccatc tcctcaactg
     2461 ccgccagtca ccagcggacc ttcgtcgtcg aggtgatggg gcgacgctgc ggctacctcg
     2521 cactcacggc cgccatcgcc ggtggcgccg acttcgccct catcccggaa gcaccccccg
     2581 gagacggctg ggaggaggag ctctgcgaga gcctgcgacg ggcccgcgaa cgcggtcgtc
     2641 gcgagtcgat cgtcgtcgtc gccgagggcg cgcgggaccg ctccggtgag ccgatcaccg
     2701 ccgaccggat ctgcgccgtc ctcgaggaac gcctcggcga gcaggcccga gtcaccatcc
     2761 tcggccacgt ccaacgcggc ggcagcccca gcgccttcga ccggtcgatg gccacgatga
     2821 tgggctacga cgccgtcgac caggtgctca ccgccggacc caatgatggc gcgcaactcg
     2881 ttggcgtgca ccgcaatcgg gtgcgccggg tgccactcat gcgagccgtt caggagaacc
     2941 aggccgtcgc tcggatggtc gacgagggtc actacgagca ggccctggca gcccgcggtg
     3001 acagctatgc cgacctgttc gggttgctcc gcaccctgtc gcacccccgg acgaccctgc
     3061 ccgccgaccc caagcggatc gccgtcctgc atgccggcgg gctggccccc ggcatgaatg
     3121 ccgccgtccg ggccgtcacc ctgctcggct ccgacgccgg ccacgagatc ctcggtgtcg
     3181 acggcgggtt caccggcctg tgcgacggtc gccttcgcac cctcacaccg cgcgacgtgg
     3241 acggctgggc ggggttaggc ggggccgagc tcggctcgcg tcgcggggtg ctggcccccg
     3301 agcagttgta tgccgtcgcc cgcgccgttg acacccaccg aatcgatgcg ctgctcgtcg
     3361 tcggcggact cgacgggtat gagagcgccc aacggatcgt tcgcgaacgc ggccgctatc
     3421 cgtcgctgga catccccgtc gtctgcctgc ccgccacaat cgacaacaac ctccccggca
     3481 ccgagctgac cgtcggcgcc gacaccgccc tcaacgcgat cgtcgaggcc atcgaccgga
     3541 tcaagcagtc ggccctggcg gcgcaccgct gcttcgtcat cgaggtcatg ggccgcgagt
     3601 gcggctacct caccctcatg tcggccctgt cggccggcgc cgagcgcgcc tacctcccgg
     3661 aggacgacct caccctcacg gacctcgccg ccgacgtccg gtcgatgacc cagcggttcc
     3721 gcgacgggca cgggctgttc ctcgcgatcc gcgccgaggg cgcccatccc tattacacga
     3781 ccgatgtgct ggcccggatc ttcgaggccg aggccgacga cctctatgac gtgcgccaag
     3841 ccgtcctcgg gcacgtccag cagggcggca acccttcacc gttcgaccgg gtgctcgccg
     3901 cgcgcttggc cgccgacgcg gtggccgccg tgtctgcagc actcggcgct ggacgctcgg
     3961 atgccgtggc gatcggcctg gacaacgggg agatcgctcg cactccgctg cgccgcgtgg
     4021 acgacctcgt cgactgggcg cgtcgccgcc cgcgcgacca gtggtggttg cggctgcggc
     4081 gcgtgatgcg ggaccttgcc gagcccgccg tggacacccg gcataccccc tgagcgaccc
     4141 ggcccgaacg cgggatgcgg ggaacccggc gggtatgccg tacggttgag gcagcagccc
     4201 ggccagtctc gcttcgggtt gcctggtgac gttgtagcga tctgagccgc accgctggtg
     4261 gaggagtcca cgagtcgacg tgcgccggcg agacggtccc accttccacg acggagattt
     4321 ttccttgtct atcaatgctt ttgctgctct cggcgtgccc gcctcgctgg cggacctgct
     4381 cgccaaggcc ggtatcgaca ccccgacgcc catccaagcg gccacgcttc ccgactcgtt
     4441 ggccgggcgc gacctgctcg gccgcggccg caccggatcg gggaagacct acgccttcct
     4501 cctgccgatc ctcgcgcggc ttgccccgaa cggagtggct ccgcgcaacg cccgcacccc
     4561 ccgtgcaccc cgcgccctca tcgtcgtccc cacgcgagag ctggcggtcc agatcgaggc
     4621 cgccctcacc ccgctggctc gcttggccgg cctgaactcg ctgaccatct tcggcggcgt
     4681 cggccagtcg ccccaggtgc gtgccctgcg tgccggcgtc gacattgtca tcgcttgccc
     4741 gggtcggttg gaggacctca tcggccaagg cgagtgccgc ctggaccggg tcgaggtgac
     4801 cgtcctcgat gaggccgacc acatgtctga cctcggcttc cttcccgcgg ttcgacgcct
     4861 gctcgagctc acccctgcga gcggtcagcg gctgctcttc tcggccaccc tcgacggggc
     4921 agtgaccgtc atcgtcaagc gctacctgcg caacccggtc acccacgagg ccgactcgcc
     4981 cgagtcggcg actccggacg tcgagcacca cgtgttgcag gtcgactccg agcagcggat
     5041 gccagtgctc gtggatctga ccagtggttc gcgccgctcg gtcgtcttca cgcgcaccaa
     5101 gcaccgggcc aagaccctcg cccgcaagct caacgcgctc ggagtgtcgt cggtcgagtt
     5161 gcacggcaac ctcgcccaga acgcccgcac ccgtaacctc gcggccttcc actccggcga
     5221 ggcgagcacg ctggtggcca ccgacatcgc tgcgcgcggg atccacgtcg acgacgtcga
     5281 gttggtcatc cacgccgacc cgccgagcga gttcaaggcc tatgtccacc gctccggccg
     5341 caccgcgcgc gccggcgcca agggcacggt catcaccctc atggagcggg agcaggccgc
     5401 cgaggtccgc gccctcgtca gggcagccgg gatccgtccg acgacgacct cggtcgggct
     5461 cggtcacccg ttgctgtccg agctggtgcc gggcgagcgc cggctagtcc cggtctccgc
     5521 tcgacccgcc gaaaccgttg ccgcaccgga gggccgcggt agtggcaccg gcggtggcgg
     5581 gcgtggcgca cgctcgggtc gctcgggctc aggcggctcg gcttctccgt cggccgggtc
     5641 cggccgtggt ggcgctgctc gctccggtgg cactcgtggc tcggcccccc gctcaggcgc
     5701 gccgcgtgcg ggtgggtccg ctccgggcgc cccgcgctcg gacgccggac gctccggcgc
     5761 tccgcgttcg ggatccgcgc gttccggatc cgcgggcggg cagcgttccg gcgccgcgcg
     5821 cgccggaagc ctctcggctg cccgcatgag tgagcagtcc ggcggccgtc gccgctccgg
     5881 tcgctgacct ctccaacaac agcccccatc accggggccg cgggagtgcc cgtatgccgc
     5941 gtgggcggca tacggtgatg gcgtgagcca gccacccccg gtgccgaccc tgctcgtcgt
     6001 ccaccacacg acctcgcccg cgctcgaatc gatgctcgac gcagtcctgg cgggcacccg
     6061 cgcggacggc atcgacgggg tcgacgtggt cgtgcgaccg gcgctggcgg ccaccgcctc
     6121 cgatctgctg gctgctggag cggtgttgct ggggacgccg gccaacatcg gctacatgtc
     6181 cggagccctg aagcacttct tcgatcaggt ctactacccc tgtctgcacg cgacggtggg
     6241 cacggcatac gggctctatg tccacggcaa cctcgacacg gtcggcgcgg cccgatcggt
     6301 ccacgcgatc gcgaccggat tgcagtggcg ccgggcggcc gacgatgtcg tcgtcctcgg
     6361 tccccccgac gcgcaggcgt tggagtcctg ttggaatctc ggcgccaccc tcgccgcctc
     6421 gctgatgccc cagtagcgct tctccgattg tggaagcccg acccggtcag ccggggaggt
     6481 tgccggtgtc tccctcgttg gtgccctcgt cgtgaacg
//
LOCUS       DAOMZY010000200         6515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_11108_length_6515_cov_6.176471, whole genome shotgun sequence.
ACCESSION   DAOMZY010000200 DAOMZY010000000
VERSION     DAOMZY010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6515
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11108_length_6515_cov_6.176471"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..450)
                     /locus_tag="PLK64_11265"
     CDS             complement(94..450)
                     /locus_tag="PLK64_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPV80372.1"
                     /translation="MTIARNPVVVLDCPDPKALAEFYGALLDWEVKGDDGWWSVRAEY
                     GHTIGFQKVEGFTAPRWPGQEVPQQMHIDVDVDDLDTAEAAVLGLGATKHDHQPGTTF
                     RVFLDPAGHPFCLCQG"
     gene            504..1487
                     /locus_tag="PLK64_11270"
     CDS             504..1487
                     /locus_tag="PLK64_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HPV80373.1"
                     /translation="MPSGSPAARALVALEIIERRPGISAADLAERLGVTDRAARRYVA
                     TLREAGIPVEAARGRDGGYRLGRGIRLPPLHFGASEALALVMAALDGHHDVSDPESPV
                     GSAIARILRALPTAVAAQADSMRRATSPVPDRSAARPDPEITAALVEVCSEGRLARLR
                     YRTEAGRRLEVEVEPWFVVVRHRRWYLLCRLLPTQEIRAYRVDRVDAVTPLAGRFDPP
                     RGVDPVALLESHLASGWPYDVVTVIDAPIDEVSRSLPTHLGRLEALEDGTTRLVGSTS
                     IPEMYAQGLSLCPAPFRVQDGDEVRAAVLALGQRLLAAGSGIPSPHEAATH"
     gene            complement(1524..1967)
                     /locus_tag="PLK64_11275"
                     /pseudo
     CDS             complement(1524..1967)
                     /locus_tag="PLK64_11275"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            1971..2321
                     /locus_tag="PLK64_11280"
     CDS             1971..2321
                     /locus_tag="PLK64_11280"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV80374.1"
                     /translation="MDARYGALLAIVSDMLADRELVSVAQVEQRHHVTSRTLQRWFMH
                     YVGVGPKWVLARYRMHDVVTAIDGGYAGSLTELAHRYGWYDQSHFTRDFTTLVSIPPS
                     RYRDERTSRIHDMT"
     gene            2410..2640
                     /locus_tag="PLK64_11285"
     CDS             2410..2640
                     /locus_tag="PLK64_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HPV80375.1"
                     /translation="MSTEILQVRDVPSEDAQILRARAAARKMSLSSYLRELIRDDASR
                     PELGAVLERIASRAGVEADGEDIRGFIAEGRR"
     gene            2643..3050
                     /locus_tag="PLK64_11290"
     CDS             2643..3050
                     /locus_tag="PLK64_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPV80376.1"
                     /translation="MVVVDASIVVRLLQNRRGDAALRERFSRERRLHAPALIDAEVTS
                     ALRGLLMTSKVAIRITRERAGEMLDDYADLPIERHLLQPLQRRVLELRDNFTAYDAFY
                     VALAEALDLPLLTDDGKFARSTSQPSLIETWPS"
     gene            3225..3419
                     /locus_tag="PLK64_11295"
                     /pseudo
     CDS             3225..3419
                     /locus_tag="PLK64_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020015614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
     gene            3657..4928
                     /locus_tag="PLK64_11300"
     CDS             3657..4928
                     /locus_tag="PLK64_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HPV80377.1"
                     /translation="MSLLDHAIWWHVYPLGFVGASIRPTADHLNELAHPNQAPTPGSL
                     ISWLDYAVGLGANGLQLGPVFASTSHGYDTVDHLRIDPRLGGEEMFDALVAACQQRGV
                     LLMLDGVFNHVGAQHPLVRRALAEGPDGELAGLFRLDWTPEGGRPAGWEGFTDLVEFN
                     HDDPRAVDLVVEVMEHWLARGISGWRLDVAYAVPLAFWRSVTDRVRAAYPSAVFVGEV
                     IHGDYRAFVSEGGLDSVTEYELWKAIWSSLVDHNPWEIGHTLERHDALLQSFIPMTFV
                     GNHDVTRIATKVGDAGAVVALVLLMTVGGTPSLYYGDEQAFRGEKTERLGGDDEVRPA
                     FPAAPDALAAEGWWMYRTHRELIALRRRHSWLQRARTSVVNKEGSLITYVSTGVGETE
                     QVTVVLDLGEQPSAVVTDHAGVEIFRFPSAP"
     gene            4978..5361
                     /locus_tag="PLK64_11305"
     CDS             4978..5361
                     /locus_tag="PLK64_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020015348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HPV80378.1"
                     /translation="MADRPDVPDAMLERLRAVAAGLPACHEEGAWVGTRWKVANATVA
                     HIFGGEDGLFRITLRGDPDEVMAFEHLGPPYFRSDWGRNVIGLVLDDTTDWVEVAELF
                     TISWFIQAPKHLAEQVDLPAAPGHG"
     gene            complement(5392..5814)
                     /locus_tag="PLK64_11310"
     CDS             complement(5392..5814)
                     /locus_tag="PLK64_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HPV80379.1"
                     /translation="MSDEALRDILAIPGGRGVLTTIKRDGRPQLSVVFHRWDADASTL
                     SISVTDDRAKTRNLRRDPRASYLVMGATPWKYVVAEAEAELGEVTRDPHDAVADDLVE
                     LYRALSGEHPDWEEFRAAMVAQHRLVLRLHVTRVYGQA"
     gene            complement(5966..>6515)
                     /locus_tag="PLK64_11315"
     CDS             complement(5966..>6515)
                     /locus_tag="PLK64_11315"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HPV80380.1"
                     /translation="TALLRGAFVAQGLLTEDSAIDNTIDYSTIGAAATLTAPDGTVTQ
                     VPISANRALSVDGVAIAADPHLLTVSIPGHFTSYVPVASSFVRPEGLGGRVMAPTVQA
                     AAGDVNNDSVIDILDAIAIRDARGTSTRAADINFDGTVDAADLRFVVINFLLRNPEAD
                     TAPYPQSTYRGVTLEAVKATFN"
BASE COUNT          967 a         2210 c         2272 g         1066 t
ORIGIN      
        1 cgccctgacc tcgccgcagt tgccttgccg gagaaggagc gcggcagctg cggcgagttc
       61 gaggcccgga cctttggcgg gcggcctagc gcgtcagcct tggcagaggc agaagggatg
      121 gcctgccggg tcgaggaaca cccggaaggt cgtccccggc tggtggtcat gcttggtggc
      181 gcccagcccg aggacggccg cctccgcagt gtcgaggtcg tcgacgtcga catcgatgtg
      241 catctgctga ggcacctcct gcccgggcca acgaggagcg gtgaacccct ccaccttctg
      301 gaagccgatg gtgtggccat actcggcgcg cacggaccac cagccgtcgt cccccttcac
      361 ctcccagtcg aggagggcgc cgtagaactc cgccagcgct ttggggtcgg ggcagtcgag
      421 gacgacaacg ggattgcgag cgatagtcat ggggagacgg taaagacata agcggacgtt
      481 ctgcgtccgg ataagatttc ggtatgccgt ctgggtcacc ggccgcccgc gccctggtgg
      541 cgctcgagat catcgagcgc cgacccggaa tcagcgcagc cgatttggcg gaacgcctcg
      601 gcgtgaccga tcgggctgcc cgccggtacg tcgccacgct tcgcgaggcc gggatccccg
      661 tcgaggcggc tcgaggaagg gatggcggtt accggctggg ccgaggcatc cggcttccgc
      721 cactgcactt cggggcgtcg gaggccctgg ccctggtgat ggctgcgctt gacgggcatc
      781 acgatgtgtc tgatcccgaa tcccccgtgg gctcggcgat cgctcggatc ctgcgcgccc
      841 tgccgacagc ggttgcggcg caggcggatt cgatgcggag ggcaacttcg ccggtgcccg
      901 accgctcggc cgctcggccg gatccggaga tcacggcagc tctcgtggag gtgtgcagcg
      961 agggccggct ggcgcgcctc cgctaccgga cagaggcagg tcgccggctg gaggtcgagg
     1021 tcgagccctg gttcgtcgtc gtgagacacc ggcggtggta cctcctgtgc cgactcttgc
     1081 ccacgcagga aatcagggcc taccgggtgg accgagtcga cgccgtcacc ccgctggcgg
     1141 gacggttcga cccgccccgg ggcgtcgatc ccgtcgccct gttggagagc catctggcct
     1201 cggggtggcc ctacgacgtc gtcaccgtca tcgacgcgcc catcgatgag gtctcccgat
     1261 ccctgcccac gcacctcggg cggctggaag cgctcgagga cggcacgacc cggctggtcg
     1321 ggtccacgag tattccggag atgtacgccc agggactctc cctctgtccc gcccctttcc
     1381 gggtgcagga cggcgacgaa gtccgcgcag ccgtcctcgc cctcgggcaa cgcctgctcg
     1441 cggcgggtag cggcatacca agtccgcatg aggcagccac ccactagtcg ttcgcgaacc
     1501 gcgcgtgggc cgagcggttg gccccagtta gcggcttggg tgagacagaa ggggtgaccg
     1561 ctcgggtcga gcaggacccg ccaactctcg cgcggctgct cgacgggaag cgtcgcgccg
     1621 aactccacgc accgcgccgc cgcagcgtcg aggtcatcga ccgcgaggtc caggtgaaac
     1681 tgcttgctgc cgtgctgatt cggccaggtc gttgtcaccg gcgtggtcac gtcccgcttc
     1741 gacgtcgaca tccgaggccg cgggtgggtc ttcggcgtca aattccgacc gggaggtctc
     1801 gtcgcgctca ccggcgtcgg tccggcgcgg gcctgggcgg accggaccct cccagccgcg
     1861 gggatcgttc cggacgatgt ggcgcagcac tggccggtct cgacgccgac ggtgacccgg
     1921 gcgggtggct cgcgacgacg gaggccgcgc tgggcggcat atctcatcgc gtggacgccc
     1981 gctatggtgc gctgctcgcg atcgtctcgg acatgctggc cgatcgggag ctcgtcagcg
     2041 tggcccaggt cgagcagcgc catcacgtga ccagccgcac gttgcagagg tggttcatgc
     2101 actacgtggg ggtggggccg aagtgggtgc tggcccgcta ccggatgcac gacgtcgtca
     2161 ccgcgatcga cggcgggtat gccggttcgc tcaccgagct cgcccaccgc tacggctggt
     2221 atgaccagtc gcatttcacc cgcgacttca ccaccctcgt gagtattcct cccagccggt
     2281 accgcgacga gcgaacgagc cgcatccacg atatgacgtg acgtcgcccg acgtgcgacc
     2341 tggcccagga gcgagggcag ccgagagact gcatgcagtc tgcatgcgca ggcgatagaa
     2401 tcctggttca tgagcacaga gatcttgcag gtccgcgacg tgccgagcga ggacgcccag
     2461 atcctgcggg cccgtgcggc agcgcgcaag atgtcgctgt cgagctacct gcgtgagctc
     2521 atccgcgacg acgcctcccg gcccgagttg ggggcggtcc tggagcggat cgcgtcgcgg
     2581 gccggtgtgg aggccgacgg cgaggacatc cgaggcttca tcgccgaggg acgccggtag
     2641 cggtggtcgt cgtcgacgcc tcgatcgtcg tgcggctcct gcagaaccgt cggggagatg
     2701 cggccctgcg tgagcgattc agtcgggagc ggcgtcttca tgccccggcg ttgattgacg
     2761 ccgaggtgac gtccgccctt cgggggttgc tcatgacgtc gaaggtggcg atccgcatca
     2821 ctcgggaacg cgccggggag atgctcgatg actacgccga cctcccgatc gagcgccacc
     2881 tcctgcagcc actacagcga cgggtcctcg agctgcgcga caacttcact gcctatgacg
     2941 ccttctacgt cgccctcgcc gaagccctcg acctgccgtt gctgacggac gatggcaagt
     3001 tcgcccggtc cacatcccaa ccatccctca tcgagacctg gccctcgtga tggggcgcac
     3061 cgcttggtgg ggaaatgagg tcctccaggc caacggcgct cggtcgagat acgccatttg
     3121 ccagatgtgg gtcgctacct cagcgaacga atcttgtgga tgtcggcagg accacctgcc
     3181 acgtctacct ggccccgcgg catacctcct cgcctgcaac gtcggctctg accgcgcgtg
     3241 aggcagaggt gctcgggctg ctcgacgagg gccggtccaa cggcgagatc gggcgagcgt
     3301 tgtttgtcag caccaagacg gccagcgtcc atgtgtcgaa catcctggcc aagctcggcg
     3361 cagcctcccg cggcgaggcc gtggcgttgg cccgagcggc gggcttgctg gagcagtgag
     3421 tggtgtgaca aatgacggaa ttgtcggcct gttgaccacg tgactggcag ggccgcccgt
     3481 gttgaaggcg ccgtcgttga cgacacgtgg tgggccgctg gggccggatc ccgcgtccgt
     3541 cggagacccc actcttggaa gcatctgagc agcgccgcgc acccctgact gccgggctgg
     3601 cggagtgtgt ctgccagtag cgcagagtgg ggatgcagcg ctggcaggat gacgggatgt
     3661 cgcttctcga ccacgccatc tggtggcacg tctacccgct cggtttcgtg ggtgcctcga
     3721 tccggccgac ggctgaccac ctcaacgagt tggcacaccc caaccaagcg ccgacccccg
     3781 gctccctgat ctcctggctc gactatgcgg tcggcctcgg cgccaacggg ctccagctcg
     3841 ggccggtctt cgcctcgacc agccacggct acgacaccgt cgaccacctg cgcattgacc
     3901 cgcgcctcgg cggcgaggag atgttcgacg ccctcgttgc cgcgtgccaa cagcgcggcg
     3961 tcctcctcat gctcgacggc gtcttcaacc atgtcggcgc gcagcacccg ctcgtccgcc
     4021 gcgccctggc cgaggggccc gacggcgagc tcgccggcct cttccgcctc gactggactc
     4081 ccgagggcgg ccggcccgcc ggctgggagg gtttcaccga cctcgtcgag ttcaaccatg
     4141 acgacccgcg ggccgtcgac ctcgtcgtcg aggtcatgga gcactggctc gcgcgcggca
     4201 tttcggggtg gcgactggac gtcgcgtatg ccgtgccgct cgccttctgg cgctccgtca
     4261 cggaccgggt gcgggcggca tacccctcgg cggtgtttgt cggcgaggtg atccacggtg
     4321 actaccgcgc gttcgtctcc gagggcgggc tcgactcggt gaccgagtac gagctgtgga
     4381 aggcgatctg gagctccctc gtcgaccaca atccgtggga gatcgggcac acgcttgagc
     4441 ggcacgacgc gctcctacag agcttcatcc cgatgacctt cgtcggcaac cacgacgtga
     4501 cccgcatcgc cacgaaggtc ggcgacgccg gcgcggtcgt cgccctcgtc ctgctgatga
     4561 ccgtcggcgg caccccctcg ctctactacg gcgacgagca ggcctttcgg ggcgagaaga
     4621 ccgagcggct cggcggcgat gacgaggtcc ggcccgcctt ccctgctgcg ccggacgcgc
     4681 tcgctgccga ggggtggtgg atgtatcgga cccatcggga gctgatcgcg ttgcgtcgcc
     4741 gtcattcgtg gctgcaacgg gcgcgaacct cggtggtgaa caaggagggc agcctcatca
     4801 cctacgtgtc gacgggcgtc ggcgagaccg agcaggtcac ggtcgtcctc gacctggggg
     4861 agcagccctc ggcagttgtc actgaccacg cgggggtcga gatcttccgc ttcccatcgg
     4921 ccccctgaac cggtcgcttc ggtgaccggt gtcggtgccg accggcagac tggtccggtg
     4981 gccgaccgtc ccgacgttcc cgacgcgatg cttgagcggc tgcgcgccgt ggccgccggc
     5041 ctgcccgcgt gtcacgaaga gggtgcctgg gtcggcactc ggtggaaggt cgccaacgcg
     5101 acggtcgccc acatcttcgg cggcgaggac gggctcttcc ggatcaccct gcgcggcgac
     5161 cccgacgagg tgatggcgtt cgaacacctg gggccgccct atttccgctc agattggggc
     5221 cgcaacgtca tcggcttggt gctggacgac accaccgatt gggtcgaggt cgccgagctc
     5281 ttcacgatct cgtggttcat ccaggcaccc aagcacctcg ccgagcaggt cgacctcccg
     5341 gccgcgcccg gtcacggctg acccgtcgca gccggacgcg cggcataccc ctcaggcttg
     5401 gccgtaaacc cgggtgacgt gcagccgcag caccaggcga tgctgggcca ccatcgccgc
     5461 ccggaactcc tcccagtcgg ggtgctcgcc actgagcgcg cggtagagct cgacgaggtc
     5521 gtcggccacc gcgtcgtggg gatcacgcgt cacctcgccc aactccgcct cggcctcagc
     5581 tacgacgtac ttccacgggg tcgcgcccat gacgaggtag ctggcccgag gatcgcgccg
     5641 caggttgcgg gtcttggcgc gatcgtcggt caccgagatc gacagggtgc tcgcgtcggc
     5701 gtcccagcgg tggaagacga ccgacaactg cgggcgcccg tcgcgcttga tagtggtgag
     5761 gaccccgcgg ccgccgggga tcgccaggat gtcgcgcagt gcctcgtcgc tcatgaactc
     5821 cacggtatgc cgtgcaggtc gccttcccgc cgaccccgat ggagctgatt ggaggtttgt
     5881 ccggtcggac gtgccgacca gatatacctc cacccaacga atgagcccgg tgggaagagg
     5941 ttcccaccgg gctcatcgtg tgctgtcagt tgaaggtggc cttcaccgct tcgagagtga
     6001 cgccgcggta ggtcgactgc gggtaggggg ccgtgtccgc ctccgggttg cgaaggagga
     6061 agttgatcac cacgaacctc aggtcggcgg cgtcgacggt gccgtcgaag ttgatgtccg
     6121 cggcccgggt cgaggtgcca cgtgcatccc ggatggcgat ggcgtcgagg atgtcaatga
     6181 cgctgtcgtt gttgacgtca ccggcagcag cctgcaccgt gggagccatc acccgacctc
     6241 cgaggccctc gggccggacg aaggacgagg cgaccggcac gtagctggtg aagtggccag
     6301 ggatggacac ggtcagcagg tgcgggtcgg cggcgatggc caccccgtcg accgagagcg
     6361 cccggttggc cgaaatcggc acctgggtca ccgtcccgtc gggggcggtc agggtcgccg
     6421 cagcaccgat cgtcgagtag tcgatcgtgt tgtcgatggc gctgtcctcg gtgagcagcc
     6481 cctgggcgac gaacgcgccc cgcaacaacg cggtg
//
LOCUS       DAOMZY010000201         6482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_11181_length_6482_cov_6.292672, whole genome shotgun sequence.
ACCESSION   DAOMZY010000201 DAOMZY010000000
VERSION     DAOMZY010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6482
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11181_length_6482_cov_6.292672"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="PLK64_11320"
     CDS             complement(<1..801)
                     /locus_tag="PLK64_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV80381.1"
                     /translation="MSTSHGVTHDHGQTKKATASGWIGSALEYYDFFIYAQAAALVFP
                     SIFFDKNDPKMGIIGALGTYAVGYVARPIGAFVLGAWGDKHGRKNVLVLCMLLMGAST
                     FLVAILPTYQQVGFLAPALLLVLRLVQGFAVAGEISGASSMILEHAPFGRRGYFGSFT
                     LQGTQFGQILAAAIFLPLATFMSDESFKSWGWRIPFLLSVFVVFAGYVIRRRVEETHA
                     FESEKAQGNVAQTPIAEAFRDSGMSILRVFMMMLNNCIMNTRRIDMPLS"
     gene            complement(960..1826)
                     /locus_tag="PLK64_11325"
     CDS             complement(960..1826)
                     /locus_tag="PLK64_11325"
                     /inference="COORDINATES: protein motif:HMM:NF009201.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPV80382.1"
                     /translation="MSTEPLVTHSLFLIGEGIGKSRTPALHEREGASLGMPVSYRIVD
                     ALELGLTAADLPDVLTWAKRLGCSGFNVTHPFKQAIIPYLDELSDRATALGAVNTVAI
                     RDGRTIGHNTDWCGFQRPFQAQLPDVGHRAVVQVGSGGAGAAVAYALLDHGANPLILH
                     DFFPERAQELADRMAAIFGADRVEVGTDLAASMARAEGVVNCTPTGMATHPGCAVPEE
                     LITPEHWVAEIVYFPLETELLRVARAKGCRTVDGGGMAAEQAVEAFYQFTGVHPDAQR
                     MHRHLRDMVGGA"
     gene            complement(1907..2731)
                     /locus_tag="PLK64_11330"
     CDS             complement(1907..2731)
                     /locus_tag="PLK64_11330"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPV80383.1"
                     /translation="MGTAAPQRYSLAHLTILDTPTPELVRIAHRTGYDYVSPRLICHG
                     LPGDDYSLATNPTLLAQTRAALADTGIPVHDIELARIVEGLDPRVYEPEIAVGAELGA
                     TGVLTSIWVPDRPFYLDAFGRLADLVASYGMTLNLEFVPIAAVRNLAGAVDVLRETQR
                     PNARLMIDLHHFHRSRDRVEDLDGLPPEWFEFCHLCDAPGEIPADVEKMTHILRAARS
                     YVGEGGINPAATLAHLPPLIYSIELPNEREEVERGAEGHARRCLETAKAYFAQPAA"
     gene            complement(2741..4507)
                     /locus_tag="PLK64_11335"
     CDS             complement(2741..4507)
                     /locus_tag="PLK64_11335"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HPV80384.1"
                     /translation="MTTDLVSHQLVRYLEHRGVRHVFGLCGHTNIAVLAALSRSTQIE
                     FVNTRHEQIASHAADGYARATKKASVVLSHLGPGLTNAATGVANAALDSIPMVVIAGD
                     VPSHYYGKHPHQEVNLHADADQWQIYRPFVKRAWRVDSAHLFPEILDKAFALAESGRP
                     GPVLVDVPMDIFSAQIDVALMDRVMTGTHELVRPSLDEATAETIVGNLLAAERPLLYI
                     GGGVVLADATDELAELVDHLQIPVAHTLMGKGAVADDNPCVLGMTGFWGTEFINDMTR
                     NADWILALGTRFSEADSSSWEEAYTFRMPPTRLMHIDIDPAELGRNYPLALGAVADLK
                     PALAALVRAAKKLAPAGVQRTEVLADIQRNRAAFKATCQEGATSDAWPMRPERILADV
                     RAVLPRDAIITTDVGWNKNGLAQQMDILTPGSVFTPGGFATMGFGAPAALGAKIACPD
                     RVVVSLVGDGGFGQNPALLYTAREKGIAAIWVVMNNNAFGTIAGLEKAHYDTTYGTVF
                     ENADGPVETDFAAIAAAYGVAGIRVETAADFRPALERAVAMNVPVVIDVRMVNVPTPT
                     AGHWNIMDIYSPGKSIHHASTD"
     gene            complement(4504..5385)
                     /locus_tag="PLK64_11340"
     CDS             complement(4504..5385)
                     /locus_tag="PLK64_11340"
                     /EC_number="4.1.3.34"
                     /inference="COORDINATES: protein motif:HMM:NF004864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HPV80385.1"
                     /translation="MSERTAQQLEAVVESAQDWWSTDIVDIHPGYIAMRGYPIEELIG
                     HVTFPEMIWLMLRGELPTRPQARLMEAALVSAVDHGPHAPSIAISRMAMTCGVPLNGA
                     MASAINVLDDVHGGAGQQCMELYSEIAARVDGGAALASATAEVLGEWRTQGVRYVPGF
                     GHRWHPLDPRAPRLLGLVDDAVAAGDVDGRYAAIGRAVEDAISAGRPARIPMNIDGAT
                     AVIYSELGFATELGRGIFILSRSVGILAHAWEQHSQGGRIKGPMPKTIPYRYTGPARR
                     AVPDLDLTEPTADKESR"
     gene            complement(5466..>6482)
                     /locus_tag="PLK64_11345"
     CDS             complement(5466..>6482)
                     /locus_tag="PLK64_11345"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPV80386.1"
                     /translation="IFSPHPRSKKTTFETVRLMREALEREGAPADILQCITLPSIPVS
                     QALMARSDLVIATGGQPMVRAAYSSGTPAYGVGAGNSTMIIDETADVAIAARNSRISK
                     TSDFGSGCSADGNLIIHESIWDEMITALQAEGGYLAEGSERDALAAVMWDAEKHRLPD
                     SVAVAPQKLARLAGFEIPTDRTFIFVKGDGISREHVFSMEKLTTLLAVHRYEGEFENA
                     LDAMRAIYEVGGKGHSCGIYSHDADHIHRLGLAAPVSRIMVRQPQSKANAGAFNNGMP
                     MTSSMGCGTWGGNVTSENVHLKHYLNTTWVSVPIPEDKPSDEELFGDFYDATLEPEGE
                     VITS"
BASE COUNT         1030 a         2025 c         2358 g         1069 t
ORIGIN      
        1 cgacagcggc atgtcgatcc tgcgggtgtt catgatgcag ttgttcaaca tcatcatgaa
       61 cacccgcagg atcgacatgc cgctatcgcg gaaggcctcg gcgatcgggg tttgggccac
      121 gttgccttgg gccttctcgg actcgaacgc gtgggtctcc tcgacgcggc ggcggatgac
      181 gtagccggcg aagacgacga agacactcag caggaacggg attcgccaac cccacgactt
      241 gaacgactcg tcggacatga aggtggcgag cggcaggaag atcgcggccg caaggatctg
      301 gccgaactgg gtgccctgca aggtgaagct gccgaagtag ccgcgtcgcc cgaacggagc
      361 atgctcaagg atcatcgaac tggcaccgga gatttcaccg gccaccgcga agccctggac
      421 caggcgcagc acgagcagca gcgccggggc gaggaagccg acctgctgat aggtcggcag
      481 gatcgcgacg aggaaggtcg aggcgcccat gaggagcatg cagagcacga ggacgttctt
      541 acgtccgtgc ttgtcacccc aagctccgag gacgaaggcg ccgatcggtc gcgcgacgta
      601 gccgacggca tacgtgccga gggcgccgat gatgcccatc ttcgggtcgt tcttgtcgaa
      661 gaagatcgac gggaagacca acgccgcggc ctgggcgtag atgaagaagt cgtagtactc
      721 caaggcgctg ccgatccagc cactggccgt ggccttcttg gtctgcccat ggtcgtgcgt
      781 gacgccatgc gatgtgctca ttggttacct ccgtgagagt ccgtccaggc gcaatgccgc
      841 ctagtccggg tggggtcact gcgggtgcaa cgtgcggtgc agagtcgggt gcaagggtct
      901 cgtgcgcaca gcttgagctg ggttcgggag cggtggtggg ccggggccga ccaggagggt
      961 caggctccgc caaccatgtc gcgcaggtgc cggtgcatac gttgagcgtc aggatggacg
     1021 cccgtgaact ggtagaacgc ctcgaccgcc tgttcagcgg ccattccccc accgtcgacc
     1081 gtgcggcagc ccttggcgcg cgccacgcgc aacagttcgg tctccagcgg gaagtacaca
     1141 atttcggcaa cccagtgctc gggggtgatg agctcctccg gcacggcgca ccccgggtgc
     1201 gtcgccatcc cggtgggggt gcagttgacg acgccttcgg cccgggccat cgaggccgcc
     1261 aggtccgtcc cgacctcgac ccggtcggcg ccgaagatgg cagccatccg gtcagccaac
     1321 tcctgggccc gctcggggaa gaagtcgtgc aggatgagcg ggttggctcc gtggtccagc
     1381 agggcgtagg cgactgcggc tcccgctccc ccggacccga cctggacgac ggcgcggtgg
     1441 ccgacatcgg gcagttgggc ctggaacggt cgttggaagc cgcaccagtc ggtgttgtgg
     1501 ccgatggtcc gcccgtcccg gatggcgacg gtgttgacgg cgccgagggc cgtggcccgg
     1561 tcggagagct catccaggta cgggatgatg gcctgcttga acgggtgggt gacgttgaac
     1621 ccactacagc ccagccgttt ggcccaggtg agcacgtcgg gcaggtcagc ggcggtgagc
     1681 cccagttcga gagcgtcgac gatccggtaa ctgaccggca taccgagcga agccccctcg
     1741 cgctcgtgca gcgcgggggt ccgtgacttc ccgatccctt ccccgatgag gaacaggctg
     1801 tgggtgacca gtggttcggt gctcatgcaa ggcctcctgc gacagtgcag tgacgggaaa
     1861 gggaaacagc gggtgaagcg gtttgtcctc gcgggaggcg aggcggtcaa gcagcgggct
     1921 gggcgaagta ggccttggcg gtctcgaggc agcggcgggc atgcccttcg gcaccgcgtt
     1981 cgacttcctc gcgttcgttg ggcaactcga tcgagtaaat gagcgggggc aggtgggcga
     2041 gggtggcggc cgggttgatc ccgccctccc cgacgtagga ccgagccgct cggaggatgt
     2101 gcgtcatctt ctcgacgtcc gcggggatct cccccggcgc gtcacagagg tgacagaact
     2161 cgaaccactc gggcggaagg ccgtcgagat cctcgacgcg gtcccgagac cggtggaagt
     2221 ggtgaaggtc gatcatcaag cgggcattgg gccgctgcgt ctcgcggagc acgtcgaccg
     2281 cgccggcgag gttgcgcacg gctgcgatcg ggacgaactc gaggttgagc gtcatcccat
     2341 agctggcgac caggtcggcc agccgaccga aggcgtcgag atagaacggg cgatctggca
     2401 cccagatgct cgtcagcact ccagtggcac ccaactcggc cccgacggcg atctcgggct
     2461 cgtagacccg cgggtccagg ccctcgacga tccgggccaa ctcgatgtcg tggaccggaa
     2521 tccccgtgtc cgccaacgcg gctcgggtct gggccaacag cgtcgggttg gtggccaggg
     2581 agtagtcatc cccggggaga ccatgacaga tcagccgggg gctgacgtag tcgtaacccg
     2641 ttcggtgagc gatccggacc aactcggggg tgggagtgtc caggatcgtc aggtgcgcca
     2701 acgagtagcg ctgcggggcc gcggtgccca tcgtctcgcc ttagtcggtg gaagcgtggt
     2761 ggatggactt gccggggctg tagatgtcca tgatgttcca gtgccccgcg gtgggtgtgg
     2821 ggacgttgac catgcggaca tcgatgacga ccgggacgtt cattgcgacg gcccgctcca
     2881 gggcggggcg gaagtcggcg gcggtctcga cccggatccc ggcgactccg tatgccgccg
     2941 cgatcgcggc gaagtcggtc tccaccggtc cgtcggcgtt ctcgaacacc gtcccgtagg
     3001 tggtgtcgta gtgggccttc tcgagcccgg cgatcgtgcc gaacgcgttg ttgttcatga
     3061 cgacccagat cgccgcgatg cccttctcgc gtgcggtgta gagcagcgcc gggttctggc
     3121 cgaacccgcc gtcaccgacg agcgacacga cgacccggtc gggacaggcg atcttggcac
     3181 cgagcgcggc aggggcaccg aagcccatcg tggcgaagcc accaggagtg aacaccgagc
     3241 cgggcgtgag gatgtccatc tgctgggcca agccgttctt gttccagccc acgtcggtcg
     3301 tgatgatggc gtcccgcggc agcaccgccc ggacgtcggc gaggatccgc tcgggccgca
     3361 ttggccaggc atcgctggtc gcgccctcct gacaggtggc cttgaaggcg gcccggttgc
     3421 gctggatgtc ggccagcact tcggtgcgct gcacgccggc cggagccagc ttcttggccg
     3481 ctcgaacgag cgcggccagc gccggcttga ggtcagccac ggcaccgagc gccagcgggt
     3541 agttgcggcc cagctccgcc gggtcgatgt cgatgtgcat gaggcgagtg ggcggcatac
     3601 ggaaggtgta agcctcctcc cacgagctgg agtcggcctc ggagaaccgg gtgcccaggg
     3661 cgaggatcca gtcggcgttg cgggtcatgt cgttgatgaa ctcggtcccc cagaaaccgg
     3721 tcatccccaa cacgcacggg ttgtcgtcgg cgaccgcgcc cttgcccatg agggtgtgag
     3781 cgaccgggat ctgcaggtgg tcgacgagct cggcgagttc atcggtggcg tcggcgagga
     3841 cgaccccgcc gccgatgtag agcagcgggc gctcggcggc gaggaggttg ccgacgatcg
     3901 tctcagcggt cgcctcgtcg agcgacgggc ggacgagctc gtgggtgccg gtcatcacgc
     3961 ggtccatgag ggcgacgtcg atctgtgccg agaagatgtc catcggcacg tcgacgagca
     4021 ccgggccggg gcggccgctc tcggcgaggg cgaacgcctt gtcgaggatc tcggggaaca
     4081 ggtgtgccga gtcgacccgc caggcccgct tgacgaacgg ccggtagatc tgccactgat
     4141 cggcgtcggc gtggaggttg acctcctggt gggggtgctt gccgtagtag tggctcggca
     4201 cgtcgcccgc gatgacgacc atggggatcg agtcgagcgc ggcattggcc accccggtag
     4261 cggcgttggt gaggccgggg cccaggtgcg agagcacgac cgaggccttc ttggtcgccc
     4321 gggcgtagcc gtcagcggcg tgcgaggcga tctgctcgtg ccgggtgttg acgaactcga
     4381 tctgggtgct gcgcgacaac gccgcgagga cggcgatgtt ggtgtggccg catagtccga
     4441 agacgtgccg aacaccgcgg tgctcgaggt agcggaccag ttggtgggag acgagatccg
     4501 tggtcatcga gactccttgt cggcggtcgg ttcagtcagg tcgaggtcgg gaaccgcacg
     4561 acgcgcgggc ccggtgtagc ggtaggggat ggtcttgggc atcgggccct tgatccgtcc
     4621 gccttggctg tgctgttccc aggcatgggc gaggatcccc accgagcggg agaggatgaa
     4681 gatcccccgc ccgagctcgg tggcgaaccc caactcgctg tagatcacgg ccgtcgcccc
     4741 gtcgatgttc atcgggatcc gggccggtcg gccggcgctg atggcgtcct ccacggcgcg
     4801 gccgatggcg gcataccggc cgtcgacgtc ccctgctgcc acggcgtcat cgaccaggcc
     4861 gagcagccgc ggagcccgag ggtccaacgg gtgccagcga tgtccgaagc cggggacgta
     4921 gcgcaccccc tgagtgcgcc actcccccag gacctcggcc gttgctgacg cgagggccgc
     4981 tcccccatcg accctcgcgg cgatctcgga gtagagctcc atgcattgct gtccggcacc
     5041 gccgtggacg tcgtccagga cgttgatggc cgaggccatc gcgccgttga gcggaacccc
     5101 acacgtcatc gccatccgcg agatggcgat cgacggggcg tgcgggccgt ggtcgacagc
     5161 cgacacgagg gccgcctcca tgagccgggc ctggggccgg gtcggcaact cgccgcgcaa
     5221 catcagccag atcatctcgg ggaaggtgac gtggccgatg agctcttcga tgggataccc
     5281 gcgcatggcg atgtagcccg ggtggatgtc gacgatgtcc gtggaccacc agtcctgggc
     5341 ggactcgacc accgcttcga gttgttgtgc ggtgcgttcg ctcatccgac tctccaacct
     5401 ctcgtttagg ggggtgacgt cgatcagggg gtatgccgtg ccgtgccgcg cgtggtgcgc
     5461 ggcggtcagg aagtgatgac ttcgccctca ggctcgagcg tcgcgtcgta gaagtcgccg
     5521 aacagttcct cgtcgctggg cttgtcctcg gggatcggca ccgacaccca cgtggtgttg
     5581 aggtagtgct tgaggtggac gttctccgag gtgacgttgc cgccccatgt gccacagccc
     5641 atcgaggagg tcatcggcat cccgttgttg aaggcgccgg cgttggcctt ggactggggc
     5701 tgacggacca tgatccggct caccggcgca gccagcccga ggcggtggat gtggtcggcg
     5761 tcatgggagt agatgccgca cgagtgcccc ttgccgccca cctcgtagat ggcccgcatg
     5821 gcgtccagcg cgttctcgaa ctcaccctcg tagcgatgga cggcgagcag ggtggtgagc
     5881 ttctccatcg agaagacgtg ctcacggctg atcccgtcac ccttgacgaa gatgaaggtc
     5941 cggtcggtgg ggatctcgaa gccggccagt cgggcgagct tctgcggggc gaccgccacc
     6001 gagtcgggca gccggtgctt ctcggcatcc cacatgactg cggccagcgc gtcacgctcg
     6061 gagccctcgg cgaggtagcc gccctcggcc tgcagggccg tgatcatctc gtcccagatc
     6121 gactcgtgga tgatgaggtt gccgtcggca gagcagcccg agccgaagtc cgaggtcttg
     6181 gagatccggg agtttcgcgc ggcgatcgcc acgtctgcgg tctcgtcgat gatcatcgtc
     6241 gagttgccgg cgccgacgcc gtaggcgggt gtccccgagc tgtaggccgc tcggaccatc
     6301 ggctgtccgc cggtcgcgat gaccaggtcg gagcgggcca tgagggcctg cgacacgggg
     6361 atgctcggca gggtgatgca ctgcaggatg tcggccggag cgccctcgcg ctcgagcgcc
     6421 tcacgcatga gccggaccgt ctcgaaggtg gtcttcttcg accgcgggtg cggcgagaag
     6481 at
//
LOCUS       DAOMZY010000202         6478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_11191_length_6478_cov_4.362136, whole genome shotgun sequence.
ACCESSION   DAOMZY010000202 DAOMZY010000000
VERSION     DAOMZY010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11191_length_6478_cov_4.362136"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>6478
                     /locus_tag="PLK64_11350"
     CDS             <1..>6478
                     /locus_tag="PLK64_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80387.1"
                     /translation="KSAHDSGNGCASFTISSVTGFRLNNFKVDNFDLFPSGTPLTALA
                     ATATTAPVLFPTADLASPLKGAVTNSATVGTSITVVFNDVNGVGLRPETIRDSDPEFD
                     LLVNGSPSTVTVNGIPTAVAGKVNTWTYTLSGALPADGLVTVRFRQAGFSDLGGSMNM
                     SEDEQFVLFTPTPTNPRPGPVAQLANPSGGGTITAAQINAQRYIDVTYTSVPAATGGV
                     LDPIIKSSIETSVAPFKITGAGIADLALDPVTGAPLIVGQPLLISGRAATATTVTYRY
                     FLKDKNTANTIGMFTSGAIQVEFFAERIRSGPVDGTYVSKNAAQSQSFTINASAPGAA
                     TTGGPIALGPLTLQGPSVGLADFGFADGMVVLTIAVGVDRASLNFGGSATNNTTTPTT
                     AQSNSGITVDLIGILGTFDLAVDVFGLLGGNVRVEPTGKWNLRVASLEAEIPNLAKLT
                     AEGILVGYDPAADDSQELIRINSATLTLPKFGITGSIRPYDPAQASNIAANNIRANDE
                     PMLGVIPGLSIRGNGFSLGTAELAYGLPPEAGGLTPSSGVVDEKITFGGILELADIRV
                     GVSGLTVTFGTETTFTGLIYIASGGAKLFPNGAFGATISDRLTADDRRPDGTADTEAF
                     RIAVTFAAGKVQAFQMSIDTLEVRLGTFVTLTARDFLLDTGAAGTSDRMVQFGSIGAK
                     VTIGALVIGGEGRNFAIRGDGTFEALQGFGVFLSVGSATGDSFQWPSFLPVRIDALGI
                     QWVDIQNAPEDFVLTLSATVTGIKGMSGLEFSGSIQGVKIAPALLAQGKFPIIAIDSI
                     AVQVKGKMFGGELEAGLVGGILRLDSNFAIIGTFDTTTPVAQRIFYLGISGGFSIAGM
                     AGLTIRIGLSELGPLQVFINVEVPGGVLLEPTTGLTLNDFSAGVEFFKTLPSIDDPLA
                     LRSSAFGLPTNLTADQWMAGLQSQVALQAKAISLNPSQSGFEAAFTSPMVITGSARVY
                     SIYTSQAVFNGLVMVKISTDGKILISGTLNFADNNISISGRLYADLSRVATGDVVVLF
                     LADVPDQVRLLTIYGKLKMGFRNSSGQEVTFDVVDVPDPAATVTAPTVNVAAPVGGGG
                     TIDVSLANAGSGERTIDVTFTPPAGASLDIASILDSAQEFTLTVNGVTRTITSGIPVP
                     IIAVTTSEGVIFVPLESDGTKVTYTYRKPGESSDTIETVVTLASLPTGSDLMTEAIRI
                     SGTTRFRYAIGSAQLPLGTVALVFTAGGVKNRDVTTTSGTTVGAGNAATTVSFTVTGV
                     SAVVVDPGPGGTVDIAVVNNRPWLTFIDVDFTVGSGRTIDLASILDLAPEISLGGSGL
                     GSAALDGTRVPIVLSVDATTGTVRVRFWTTGAFAASGTVTVTPLLRTWAFTATLSAAT
                     LASVTIPANTSVTTIAVTIPGVTADMASLNGLSFVDLDAAAPGVQLYAASGQIVTLDT
                     SRAIVWNGSAFLIPVIATSSTAPELEVEAQIQAGTVSWTSAQDSDYTGRTAAVAPATV
                     TGATTYIDVTFSPSMGALLTQGSINGDEILLTGVGAAGISLVSGGAVYLHGTTWRFAL
                     SGQFAIGAVNVNIKLDAFSDNSGRSPPAGSVLVQSFTVVGSTADAVTTTTAGALTALG
                     GQTVSRETINLLHYLQIRFVGAGGAAIDPSTIDGNEIELRDAAGTLISLAVPIRVGTT
                     DVFRYGLSADLATGRYTITILGGSFADVNGIANVTETETFTLVSPTAALTDPVRGQVT
                     YVDEFGTRGYVDITFTPAPGATLNVAEILALRPTFSGGGAESLTITSVTRQGTSNVFR
                     FAFTGTFTPGLVTMTLAAGAWRDSAGNASAATTNTFRLIAPAQSFFIELSGGLRLEAG
                     GLTSEPLIDLSASVLLEIDNNRKLFILTFEGQLSIIKLGTVGATSGRFVLDNSDPAHL
                     PPRLWGVASLQTNFDALLPYGLRLSASGTLQINLTNETKVETITLKGVGPGHTDLVQT
                     YTLKPYSFGLELVGLAVVSIPGTSTELMRIQGGLLLDISMAENPSLTMFMTGTLSYGS
                     GSVQITLASATAVLFIRTDAGHAGVAGSVTISAGGDIGLPNLALFSATGTVNLMFNTT
                     LQDQTLELPASFLPLLREGDPTSITVYAAPPDLTGEPPAGAAPAAYVKATVRAQLVFA
                     GVLVLDGYIGITAAGSGAGARARSSDP"
BASE COUNT         1048 a         2398 c         1973 g         1059 t
ORIGIN      
        1 gaagtcggcc cacgattcgg ggaacgggtg cgcgtccttc accatctcgt cggtcaccgg
       61 cttccggctc aacaacttca aggtcgacaa cttcgacctg ttcccgagcg ggaccccact
      121 gacggccttg gctgcgaccg cgacgactgc ccccgtcctc ttcccgacgg ccgacctggc
      181 cagcccgctc aagggcgccg tcaccaactc cgccacggtg ggcacgtcga tcaccgtcgt
      241 cttcaacgac gtcaacgggg tcgggctgcg tcccgaaacg atccgcgaca gcgacccaga
      301 gttcgacctg ctcgtcaacg ggtcgccgtc aacggtgacg gtcaacggca tacccactgc
      361 tgttgcgggc aaggtcaaca cctggaccta cacgctgtcc ggtgcgcttc cggccgacgg
      421 cctggtgacc gttcggttcc gccaggccgg gttctcagac ctgggcggat ccatgaacat
      481 gagcgaggac gagcagttcg tcctcttcac tccgacaccg accaacccgc gtccgggccc
      541 cgtcgcgcag ctggcgaacc cgagcggcgg cgggacgatc accgcggccc agatcaacgc
      601 ccagcgctac atcgatgtca cctacacctc ggtgccggcg gccaccgggg gagtgctcga
      661 cccgatcatc aagtcgtcca tcgagacctc ggttgcgccg ttcaagatca ccggcgcggg
      721 catcgccgac ctggcgctcg acccggtcac cggtgctccg ctgatcgtcg gtcagcccct
      781 gctcatcagc ggccgggcag cgacggcgac gacggtcacc taccgctact tcctcaagga
      841 caagaacacc gccaacacca tcggcatgtt caccagcggc gcgatccagg tggagttctt
      901 tgctgagcgg atccgcagcg gccccgtcga cgggacgtat gtcagcaaga acgcggccca
      961 gagccagtcc ttcaccatca acgcatccgc gcccggcgca gcgacgaccg gtggcccgat
     1021 cgcgctcggc ccgttgacgc tgcaagggcc gagcgtcggc ctggccgact tcgggttcgc
     1081 cgacggcatg gtcgtcctca ccattgcggt gggagtcgac agggccagcc tcaacttcgg
     1141 cggctcggcc accaacaaca cgaccactcc gacgacggcg cagtccaaca gcggcatcac
     1201 ggtcgacctc atcggcattc tcggcacctt cgacctcgcg gtcgatgtgt tcggcctgct
     1261 cggcggcaac gtccgggtcg agccgaccgg caagtggaac ctgcgggtcg cctctctcga
     1321 agccgagatc cccaacctgg ccaagctcac ggccgagggc atcctcgtcg gttatgaccc
     1381 ggcggcagac gacagccaag agctcatccg gatcaacagc gccaccctca ccttgccgaa
     1441 gttcgggatc acgggctcca ttcgccccta cgaccccgcg caggcgtcga atatcgcggc
     1501 caacaacatc cgcgccaacg acgagccgat gctgggcgtc atccctggcc tgtcgatccg
     1561 cggcaacggc ttcagcctcg gcaccgccga actcgcctat ggcctcccgc cagaggcggg
     1621 cgggctcacc ccaagtagcg gtgtcgtcga cgagaagatc accttcggcg gcatactcga
     1681 actcgctgac atccgcgtcg gggtgtccgg gctcacggtg accttcggca ccgaaacgac
     1741 tttcaccggg ctgatctaca tcgcctccgg tggggccaag ctcttcccca acggcgcgtt
     1801 cggcgccacg atctccgacc gactcacggc cgatgaccgc cggccggatg ggacggcaga
     1861 caccgaggcg ttccggatcg ccgtcacctt cgccgcaggc aaggtccagg ccttccagat
     1921 gagcatcgac acccttgagg tgcgcctcgg caccttcgtc acgctcaccg cccgggactt
     1981 ccttctcgac acgggcgctg cggggacctc cgaccggatg gtgcagttcg gctcgatcgg
     2041 cgcgaaagtc accatcggtg cgctcgtcat cggcggcgaa ggacgcaact tcgcgatccg
     2101 cggcgacggg accttcgaag cgctgcaagg tttcggagtg ttcctctccg tcgggtcggc
     2161 gacgggcgac tcgttccagt ggccgtcgtt cctcccggtc cgcatcgacg cgctcggcat
     2221 ccagtgggtc gacatccaga acgccccgga ggacttcgtc ctcacgctgt cggcgaccgt
     2281 gaccgggatc aagggcatgt cggggctgga gttcagcggg tcgatccagg gcgtcaagat
     2341 cgctccggcc ctgttggccc agggcaagtt cccgatcatc gccatcgact cgatcgccgt
     2401 ccaggtcaag ggcaagatgt tcggcggcga gttggaggct ggcctggtcg gcggcattct
     2461 gcgcctggac agcaacttcg cgatcatcgg caccttcgac acgaccaccc cggtcgcgca
     2521 gcgcatcttc tatctcggca tctctggagg cttctccatc gccgggatgg ccggcctgac
     2581 gatccggatc gggctctcgg agctcggccc gttgcaggtc ttcatcaacg tcgaagtccc
     2641 gggcggcgtc ctgctcgaac cgaccactgg gctgacgctc aacgacttct ccgcaggcgt
     2701 ggagttcttc aagaccctcc cgtcgatcga cgacccgctc gcgctgcgct cctcggcctt
     2761 cggtctcccg acgaacctca ccgccgacca gtggatggcc ggcctgcagt cccaggtcgc
     2821 cctccaggca aaggccatct cgctcaaccc gagccagagc gggttcgagg cggccttcac
     2881 ctcgccgatg gtcatcaccg gcagcgcgcg ggtctactcc atctacacct cgcaggccgt
     2941 cttcaacggt ctggtgatgg tgaagatctc gaccgacggc aagatcctca tctccggaac
     3001 gctcaacttc gccgacaaca acatctcgat cagcggtcgc ctgtatgccg atctgtcccg
     3061 tgtcgcgacc ggcgacgtcg tcgtgctgtt cctcgctgac gtgcccgacc aggtgcgcct
     3121 gctcacgatc tacggcaagt tgaagatggg cttccgcaac tcctccgggc aagaggtcac
     3181 cttcgacgtc gtcgacgttc cggacccggc tgccacggtg acggctccga cggtcaacgt
     3241 cgccgcgccg gtgggcgggg gcggcacgat cgacgtgtcc ctcgccaacg cgggtagcgg
     3301 agagcgcacg atcgatgtca cgttcacccc gccggccggg gcgagcctgg acatcgccag
     3361 catcctggac tccgcgcagg agttcaccct caccgtcaac ggcgtcacgc ggaccatcac
     3421 gagcggcata ccggtcccga tcattgccgt gaccacgtcc gaaggcgtca tcttcgtccc
     3481 tctcgagagc gacggcacga aggtgaccta cacgtaccgc aaaccggggg agagctccga
     3541 caccatcgag acggtggtga ccctcgccag cctcccgacc ggcagtgacc tgatgactga
     3601 ggcgatccgg atcagtggga cgacgcggtt ccgctacgcc atcggctcgg cgcaactgcc
     3661 gctgggcacc gtcgcgctcg tgttcaccgc gggtggcgtc aagaaccgtg acgtgacgac
     3721 gacctccggg acgacggtcg gagcaggcaa cgcggccacg accgtgtcgt tcaccgtcac
     3781 tggcgtcagc gcggtcgtcg tcgacccggg ccccggcggc accgtcgaca tcgccgtcgt
     3841 caacaaccgc ccctggctga ccttcatcga cgttgacttc accgtcggca gtggccgcac
     3901 catcgacctc gcgtccatcc tcgacctggc tcccgagatc agcctcggcg gctccgggct
     3961 cggcagcgcc gcactcgacg gcacccgagt cccgatcgtg ctgtcggtcg acgcgacgac
     4021 gggcacggtg cgcgtgcggt tctggacgac cggtgcgttc gccgcttccg gcacggtgac
     4081 cgtgactccg ctgctgcgca cctgggcctt caccgccacc ctcagcgccg ccacgctggc
     4141 gtcggtcacc atcccggcca acacgagtgt gacgaccatc gcggtcacca tcccgggcgt
     4201 cacggccgac atggcctcgc tcaacggcct ctcgttcgtt gacctcgacg ccgccgcgcc
     4261 aggcgtgcag ttgtatgccg cgtccggcca gatcgtcacg ctcgacacga gccgagccat
     4321 cgtctggaac ggcagcgcgt tcctcattcc ggtcatcgca acgtcgagca cggcccctga
     4381 gctggaggtc gaggctcaga tccaagccgg gaccgtctcg tggacgagcg cccaggacag
     4441 cgactacacc ggacggaccg ccgcggtcgc cccggcgacg gtcaccggcg ccacgaccta
     4501 catcgacgtg acgttcagcc cgtcgatggg tgccctgttg acccaggggt ccatcaacgg
     4561 cgacgagatc ctcctcaccg gtgtgggggc cgcaggcatc agcctggtgt ccggtggcgc
     4621 ggtctacctc cacggcacga cctggcggtt cgccctgtcc gggcagttcg ccatcggtgc
     4681 ggtcaacgtc aacatcaagc tcgacgcgtt cagcgacaac tccgggcgca gcccacctgc
     4741 tggctcggtc cttgtccaga gcttcacggt cgtcggctcg acggccgacg cggtgacgac
     4801 cacgaccgct ggtgccttga ccgccctagg tggtcagacg gtctcccgcg aaaccatcaa
     4861 cctcctgcac tacctccaga tccggttcgt cggtgccggt ggcgcagcca tcgaccccag
     4921 cacgatcgac ggcaacgaga tcgaattgcg cgatgccgct gggacgctca tcagcctcgc
     4981 ggtcccgatc cgggtcggaa ccaccgatgt cttccgctac ggcctgagcg ccgacctcgc
     5041 gaccgggcgc tacaccatca cgatcctcgg aggcagcttc gccgacgtca acggcatcgc
     5101 caacgtcacc gagaccgaga cgttcacgct cgtctcgccg accgccgccc tcaccgaccc
     5161 ggtgcggggc caggtgacct acgtcgacga gttcggcacc cgcgggtatg tcgacatcac
     5221 cttcactccg gcccccggcg ccaccctcaa cgtcgccgag atcctcgcgc tgcgccccac
     5281 cttctccggc ggcggcgccg agtcactcac catcacctcg gtgacccgtc agggcacgtc
     5341 caacgtcttc cgcttcgcct tcacaggcac gttcaccccc ggcctggtca ccatgactct
     5401 ggctgccggc gcgtggcgcg actcggcggg caacgcatca gcggccacga ccaacacgtt
     5461 ccgactcatc gccccggcgc agtccttctt catcgagttg tccggcgggc tgcgcctcga
     5521 ggcgggtggc ctgacgtcgg agccgctcat cgacctgtcg gccagcgtgc tgttggagat
     5581 cgacaacaac cgcaagctgt tcatcctcac cttcgagggg cagctgagca tcatcaagct
     5641 cgggactgtc ggagcgacct cggggcggtt cgtcctggac aacagcgacc ccgcgcacct
     5701 tcccccgcgg ttgtggggtg tggcctcgct gcagaccaac ttcgacgcgc tgcttcccta
     5761 cggcctgcgg ctgagcgcct cgggaaccct gcagatcaac ctcaccaacg agaccaaggt
     5821 cgagacgatc accctcaagg gcgtcggccc cgggcacacc gacctggtcc agacctacac
     5881 ccttaagccc tacagcttcg gccttgagtt ggtcgggctg gccgtcgtga gcatccccgg
     5941 cacgagcacc gagctcatgc gcatccaggg cgggctgctg ctggacatct ccatggccga
     6001 gaacccgtcg ctgacgatgt tcatgaccgg cacgctgtcc tacggctcgg gctcggtcca
     6061 gatcacgctg gcctcggcga ccgcggtgct gttcatccgc accgacgccg gacacgccgg
     6121 tgtcgcgggc agtgtgacga tctccgccgg cggcgacatc ggcctgccca acctggcgct
     6181 cttctcagcg accggcacgg tcaacctcat gttcaacacg acgctgcagg accagacgct
     6241 ggagttgccg gcctccttcc tgccgctgct gcgcgagggc gacccgacct ccatcaccgt
     6301 gtatgccgcc ccgccggacc tcaccggtga gccgcccgcg ggtgccgctc ccgcggcata
     6361 cgtcaaggcg accgtccgcg cgcagttggt ctttgcgggc gtccttgttc tcgacgggta
     6421 catcgggatc actgctgcgg gctctggcgc cggcgccaga gcccgcagca gtgatccc
//
LOCUS       DAOMZY010000203         6397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_11349_length_6397_cov_7.286660, whole genome shotgun sequence.
ACCESSION   DAOMZY010000203 DAOMZY010000000
VERSION     DAOMZY010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11349_length_6397_cov_7.286660"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="PLK64_11355"
     CDS             <1..1061
                     /locus_tag="PLK64_11355"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HPV80388.1"
                     /translation="IMDAGRLRSLVREFLAAPYGPPGSKPLVLGDRLLSKDGNDSGAD
                     VTALIDALNAKGYKLTNDGLFGPAVDAAVRDLQTKAGLTVDGIVGPATLAVLTEEEPP
                     VIVVPTPPEPEPTPEPVPTPQPKAATFKLGVANCQSYDGTKTEKAWRARAAIFAREGW
                     QIVCCTETSKAGRAVMLDELERLTKHDWKAEVLKVGAQTVAVLYDTKVFRGGRKARPA
                     DFNTAFGHGAIVVPLTVDGLGVDVAAVHIRPGSVASDAGKRSDMAKAAGVAGTWPFVL
                     GGDFNWNRPAIKGLVRVGAGLDTHDAAKLKALDNVFVRSGTEVTVTATDERLINPGSL
                     SDHRWLGYTVHLLKPATT"
     gene            1109..1246
                     /locus_tag="PLK64_11360"
     CDS             1109..1246
                     /locus_tag="PLK64_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80389.1"
                     /translation="MATKHTEAEVCVDRDAHLAAQHPHIVAAHEHLLAISHYRFLEVH
                     Q"
     gene            1255..1620
                     /locus_tag="PLK64_11365"
     CDS             1255..1620
                     /locus_tag="PLK64_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80390.1"
                     /translation="MRKALYGLIPLVVAALTTYGIFTDNLGVLWGNVTTLTVGFAFAA
                     SRATGDRFGDPEVRRALYVLVPAVVALIGGYWSVDVALWTSLAMAILGAALAGLNVDP
                     DEQNTADYQPKRIEEGRDS"
     gene            1620..1967
                     /locus_tag="PLK64_11370"
     CDS             1620..1967
                     /locus_tag="PLK64_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80391.1"
                     /translation="MRRLLAALAVAALVACSPLAPATRAQAATSASVVCSKWVSTYAG
                     TAGVTIYRGHQVCYGSGAKEYRSRIVCTLWGQYWHTRYGGWKRLGSGKDSYNSCGWPA
                     QISGRSDIKTETR"
     gene            1964..2086
                     /locus_tag="PLK64_11375"
     CDS             1964..2086
                     /locus_tag="PLK64_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80392.1"
                     /translation="MSVRKLDPPMQCIACGRTAQYVIDGTSWCRIHAPIQPKES"
     gene            2086..2442
                     /locus_tag="PLK64_11380"
     CDS             2086..2442
                     /locus_tag="PLK64_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80393.1"
                     /translation="MPRIPTSGLNSAVVAAGMTPGTTYYLSAHSADPGTTGASELSGG
                     GYARQAIVFGTATGGAIANNAAISIPNAGTTAVTHFGVWSASTGGTYLGGFPLASSVT
                     AASISVAAGAVTLTAS"
     gene            2459..2743
                     /locus_tag="PLK64_11385"
     CDS             2459..2743
                     /locus_tag="PLK64_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80394.1"
                     /translation="MVGFPTDKNTLDARAGNIAIQVRDSLDAASRLKAWLDTKTEADL
                     VALGYTSAEVAVIKSAFTDLAALRSVATAGQTVPAVNNFLFWSAQLCGVN"
     gene            2762..4318
                     /locus_tag="PLK64_11390"
     CDS             2762..4318
                     /locus_tag="PLK64_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80395.1"
                     /translation="MASPTLVAEYKTSSNWTTTTATQTASATWSSGDVLVVVAICNGS
                     SPTVGTPTVSGLTFSLVTSYSGNGGIYVYTATAVASSSGTVSATRSGNSEPWGFWVGR
                     HSGSAGVGAYAAGNGSGAPSHSLTTTADNSAIVEGIVDWNEAWTPSTGTWLTVNSVAG
                     TDVLVYTLPANLYPRLGRWADAGTAGAKTVGMSSPTGQSWRAIAVEIKGSGSAVTAAG
                     SVVISGAASGVGVAQSAGAVVVTGAATARAVAAAAGTVMVSGAATGSGVGLAAGTLSL
                     TGAATARATGQASGGITITGAAVATAAATATGTLTLAGAASATSAGSATAAGGLVLTG
                     AAQASAAATAAGTLTLAGAAVASSAGSATAAGGLVLSGSATASAQAVAAGAIVLAGAA
                     TASGGAVAAGSLALAGTAQANSAGSAQATGALILTGAASATGQAVALGGLLLVGAALA
                     RAAATASGGLTLTGAAAASGPPPDTTHADLTIHTGAASYTRITTGPASYTRITAGPAT
                     RRTIHPGRTL"
     gene            4315..4641
                     /locus_tag="PLK64_11395"
     CDS             4315..4641
                     /locus_tag="PLK64_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80396.1"
                     /translation="MKLHIQEQNYYALADLRATPAGSTTPITVPVTSWEASYDRGTTW
                     HAAQPHPDLPTTPCWLIRGPGFPGPGDTNPSDGVLVARSTTPLIRLSDSPETEIEAAE
                     IIEVWR"
     gene            complement(4738..5367)
                     /locus_tag="PLK64_11400"
     CDS             complement(4738..5367)
                     /locus_tag="PLK64_11400"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HPV80397.1"
                     /translation="MHAVGYLRVSTDQQGESGAGLEAQQRAISAEAERRGWIDLRSYT
                     DVASGKSTKRRPQLAAALTELSEGRAHVLVVAKLDRLSRSLLDFAELTARASREGWSI
                     VALDIGVDTSTVNGELVANIIMALAQWERRIIGQRTKDALGTVRRRGTKLGRPTVVTP
                     TAVAVIQAMRSAGASYRQVARTLNDQRLPTAQGGRQWYASTVRAIEQRQ"
     gene            5580..5801
                     /locus_tag="PLK64_11405"
     CDS             5580..5801
                     /locus_tag="PLK64_11405"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPV80398.1"
                     /translation="MTTALSERVAAHVRGLIEVRGLKKKALASALGITESQAYSRISG
                     RVAFEVDELPAVAQFLGCDLQDLLRSEAA"
     gene            5798..6070
                     /locus_tag="PLK64_11410"
     CDS             5798..6070
                     /locus_tag="PLK64_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80399.1"
                     /translation="MTTYTITLSSDELAFVLAAVREDLSQSVRYRDETLADEHPAADY
                     WVDRSIRLARLWQQITTDALQGYYPADPLVTAVLESSPVAIPPASG"
     gene            6194..>6397
                     /locus_tag="PLK64_11415"
     CDS             6194..>6397
                     /locus_tag="PLK64_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80400.1"
                     /translation="MSTTPLKVTTQEALDKALADGVTDLIVEAPDWAYLEIRHDGLVR
                     LFGSSRVEARESSHVEAWESSRVE"
BASE COUNT         1041 a         2330 c         2023 g         1003 t
ORIGIN      
        1 cgatcatgga cgccggccga ctccgctcgc tcgtgcgcga gttcctggcc gccccgtacg
       61 gtccgcccgg ctcgaagccg ttggtgctcg gggaccggct gttgtcgaag gacggcaacg
      121 actccggcgc ggacgtgact gccttgattg atgcgctgaa cgcgaagggc tacaagctca
      181 ccaacgacgg cctgttcggt ccggcggtcg acgccgctgt gcgtgacctg caaaccaagg
      241 cggggctcac ggtggacggc attgtcggcc cggccaccct cgccgtgctc acggaggagg
      301 aaccgcccgt gattgtcgta cccaccccac cagaaccgga gccgacgcct gagccggtgc
      361 cgacgccgca gcccaaggcg gccacgttca agctcggtgt cgcgaactgc cagtcatacg
      421 acggcaccaa gaccgagaag gcgtggcgtg cccgggctgc gatcttcgcc cgcgagggtt
      481 ggcagatcgt gtgctgcacc gaaaccagca aagccggccg ggctgtgatg ctcgacgaac
      541 tcgagcggct cacgaagcac gactggaaag cggaggtgct caaggtcggc gcgcagacag
      601 tcgcagtctt gtacgacacg aaagtgttca ggggtggccg caaggcccgc cccgctgatt
      661 tcaacaccgc tttcgggcac ggcgcgatcg tggtgccgct caccgtcgac ggcctcggcg
      721 ttgatgtggc agccgtgcac atccggcccg ggtcggtcgc ctcagacgcc ggcaagcggt
      781 ccgacatggc gaaagcggcg ggcgtggccg ggacgtggcc cttcgtgctg ggcggcgatt
      841 tcaactggaa ccggccagcc atcaagggcc tggtcagggt tggtgccggt cttgacacgc
      901 acgacgcggc caagctgaag gcgctcgaca acgtgttcgt gcgcagcggc acagaggtca
      961 ccgtcacagc aaccgacgaa cgactcatca acccgggcag cctgtcagac catcgctggc
     1021 tcggctacac ggtgcaccta ctcaaaccgg ccaccaccta acggccgtga tcatctactg
     1081 gtggacctgc ccttggtgca tggaccggat ggccaccaaa cacaccgagg ctgaggtgtg
     1141 cgtcgaccgt gacgcacacc tcgccgccca gcacccgcac attgttgccg cacatgagca
     1201 tctgctcgcc atctcccatt accgattcct ggaggttcac caatgacccc gaccatgcgc
     1261 aaagccctgt acgggctaat ccccctcgtt gtcgccgccc tcaccacgta cggcatcttc
     1321 accgacaacc tcggcgtgct gtggggcaac gtcacaaccc tgaccgtcgg cttcgcattc
     1381 gccgcatcga gggcgaccgg tgaccgcttc ggtgaccccg aggtacgccg cgccctgtac
     1441 gtcctcgtcc ccgccgtcgt cgccctcatc ggcggctact ggtcggtcga tgtggccctg
     1501 tggacctcgc tggcaatggc gatactcggt gccgcgttgg ccgggctgaa cgtggacccc
     1561 gacgaacaga acaccgccga ctaccagccc aagcggatcg aagaaggaag ggactcctga
     1621 tgcgccgcct cctcgccgcc ctggcggtcg ccgcgctggt cgcctgctcc ccgctcgctc
     1681 cggcgaccag ggcgcaggcg gccacgtcgg ccagcgtcgt ctgctcgaag tgggtcagca
     1741 cctacgccgg caccgccggg gtgaccatct accgcggcca tcaggtctgc tacgggtcgg
     1801 gcgcgaagga gtaccggtcg cggatcgtgt gcaccctgtg gggccagtac tggcacaccc
     1861 gctacggcgg gtggaagcgg ctcggctccg gcaaggacag ctacaactcg tgcggctggc
     1921 cggcgcagat cagcggccgg tccgacatca agacggagac gcgatgagcg tccgcaagct
     1981 cgacccgccc atgcagtgca tcgcctgcgg gcgcaccgcc cagtacgtca tcgacggcac
     2041 cagctggtgc cgtatccacg cacccatcca acccaaggag tcctgatgcc ccgcatcccc
     2101 acatccggcc tgaactcggc cgtcgtcgcc gccggcatga cgcccggcac cacctactac
     2161 ctgtcggccc attcggccga cccgggcacc accggcgcct ccgaactgtc gggcggcggc
     2221 tatgcccgcc aggcgatcgt tttcggcacg gccaccggcg gcgccatcgc caacaacgcc
     2281 gcgatcagca tccccaacgc tggcaccacc gcggtcaccc acttcggtgt ctggtcggcg
     2341 tcgacgggcg gcacctacct gggcggcttc ccgttggcct cgtcggtgac cgcggcgtcc
     2401 atctcggtcg ctgccggagc cgtcaccctc accgcctcct gatgaaagga cgatcctgat
     2461 ggtcggcttt cccacagaca aaaacacgct cgacgccagg gccggcaata tcgccatcca
     2521 agtacgcgat tctctcgacg cggcatcccg gctcaaggcg tggctcgaca ccaaaaccga
     2581 ggccgatttg gtcgcactcg gctacacctc tgccgaggtc gccgtcatca aatcggcatt
     2641 caccgacctg gctgcactac ggtcggtggc gacggcaggc cagacggtcc cggctgtgaa
     2701 caacttcctg ttctggtccg ctcagctctg cggcgtgaac tgatccccta gggggtggcc
     2761 tgtggcatca ccaacccttg tcgccgagta caagaccagc agcaactgga ccacgaccac
     2821 cgccacccag accgcctcag ccacatggag ttccggcgat gtgttggtcg tggtggcgat
     2881 ctgcaacggc tctagcccca cggtcggcac cccgaccgtg tcgggcctga ccttctcgct
     2941 ggtcacgtcc tatagcggta acggcggaat ctacgtctac accgctaccg ctgtcgcctc
     3001 gtcgtcgggc accgtctccg cgacccggag cggcaactct gagccgtggg gcttctgggt
     3061 cggccgacac tcgggcagtg ccggtgtcgg tgcgtatgcg gcaggcaacg gcagcggcgc
     3121 acccagccac agcctgacca ccaccgccga caactcggcc atcgttgagg gcatcgtcga
     3181 ctggaacgag gcatggaccc cctcgacggg cacctggctg acggtcaact cggtggccgg
     3241 taccgacgtg ctcgtctaca ccctgcccgc caacctctac ccgcgtctgg gccgctgggc
     3301 cgacgcaggc acagccggtg ccaaaacagt gggcatgtcc tcaccgaccg gccagtcgtg
     3361 gcgggcgatc gccgtcgaaa tcaagggtag cggttcggct gttactgcgg ccggttcggt
     3421 cgtcatctct ggtgcggcat ccggtgtggg cgtggcgcag tcggctggtg cggttgttgt
     3481 gaccggcgcc gctaccgccc gtgctgtggc agcggcggct ggcacagtca tggtgtcggg
     3541 ggctgctacg ggaagcggtg ttgggctggc ggctggcacg ctaagcctga ccggtgcagc
     3601 tactgcccgc gcaacggggc aggcgtccgg cggcatcacc atcacggggg cagccgtcgc
     3661 aactgcggca gcaaccgcga ccggcaccct caccctggcc ggcgcggcgt ccgccacgtc
     3721 ggcaggcagc gccacagccg ccggcgggct ggtcctgacc ggtgcggcgc aggcgtccgc
     3781 agcagccacg gcagccggga cgctcaccct cgcgggggca gcggtcgcca gctcggccgg
     3841 ctcagccacc gcagccggtg ggctcgtgct cagcggctcg gccacagcct cggcgcaggc
     3901 cgtcgcagcg ggcgccatcg tgctggccgg cgcggccacg gcgtccggcg gcgccgtggc
     3961 cgcaggcagc ctcgccctcg ccgggacggc tcaggccaac tcggcaggct cggcgcaggc
     4021 gaccggcgcc ctgatcctca ccggggctgc cagcgccacc gggcaggcgg tcgccctcgg
     4081 tggcctgcta ctcgtcggcg ccgccctcgc ccgagcggca gccacggcgt ccggcggtct
     4141 caccctcacg ggtgccgccg cggcgtccgg gccgccaccg gacacaaccc acgccgacct
     4201 caccatccac accggtgccg ccagctacac ccggatcacc accggcccgg ccagctacac
     4261 ccgcatcacc gccggacccg ccacccgccg caccatccac ccagggagga ctctgtgaag
     4321 ctgcacatcc aggagcagaa ctactacgcc ctagccgatc tgcgtgccac accagccggc
     4381 agcaccacac ccatcaccgt gccggtcacg tcatgggagg ccagctacga ccgcggcacc
     4441 acctggcatg ccgcccagcc gcaccccgac ctgcccacca caccctgctg gctgatccgc
     4501 ggccccggat tccccggacc cggcgacacc aacccatcgg acggcgtcct cgtcgcccgc
     4561 tccaccacgc cactcatccg gctgtccgac agccccgaaa ccgagatcga ggccgccgag
     4621 atcatcgagg tgtggcgctg acacgccgag gtcccctcta cagcaacagc ccccacgctc
     4681 cccagaccgg gagcgtgggg gctttcgtcg tgttcaggcc ttgcttggct gccgtgctca
     4741 ttgtcgctgc tcaatcgcgc gtactgtgct ggcgtaccac tgccgaccgc cttgggcggt
     4801 cggcagccgc tgatcgttga gcgtccgcgc cacctgcctg taggacgcgc ccgccgatcg
     4861 catcgcttga atgaccgcca cggcagtagg cgtgacgacg gttggccggc cgagcttggt
     4921 gcccctacgt cggacggtcc cgagcgcgtc cttggttcgc tggccgatga tgcgccgctc
     4981 ccactgagct agcgccatga tgatgttggc gaccagttcg ccgttgactg tggacgtgtc
     5041 cacgccaatg tccagcgcca cgatcgacca tccctcgcgg gacgcccggg ccgtgagttc
     5101 tgcaaagtcg agcagggacc ggctgaggcg gtcgagcttg gccaccacca ggacgtgagc
     5161 acgaccctcg gacagttccg tcagtgccgc tgcgagttgg ggcctccgct tggtgctctt
     5221 gccgctggct acgtcggtgt agctgcgcag atcgatccag ccacggcgtt ccgcttctgc
     5281 cgatatggcc cgctgttgtg cttctagtcc ggcacctgat tcgccctgct ggtcggtgga
     5341 gacccggaga taccctaccg cgtgcatcta tttcccctag ccgcgccgcg tttgggggcg
     5401 cattgttgta cacccccctt gcctgtttga gtgtactaca aacacccgtt agttgtacgg
     5461 tacgcacagg cgtaaaccta cgccagcacg ccacgctgag gtccaaccgc gtacgagaat
     5521 ctttggccaa tctgtacgga atccttgtac taggtacgag atatctgtac tgtgaggcca
     5581 tgaccacagc actgagtgag cgtgtagcgg ctcacgtccg ggggctgatc gaggttcgcg
     5641 gcctgaagaa gaaggccctg gcgtccgcac tcgggatcac cgagtcgcag gcatactcac
     5701 gcatctccgg ccgcgtggcc ttcgaggttg acgagctgcc cgccgtcgct cagttcctcg
     5761 gatgcgacct ccaagacctc ctgcggagcg aagccgcatg accacctaca ccatcaccct
     5821 gtccagcgac gagttggcct ttgtcctcgc ggccgtcagg gaggacctgt cccagagtgt
     5881 ccgctaccgg gacgaaaccc tggccgacga gcaccctgcc gccgactact gggtggaccg
     5941 gtcgatccgg ctggcccggc tgtggcagca gatcaccacc gacgcattac agggctacta
     6001 cccggccgat ccgctcgtga ccgccgtcct tgaatcgtcc ccggtagcca tccccccggc
     6061 atccgggtga ccccgagggg gccgtcgagc gatgggacaa gcacgacggc cccccaccaa
     6121 ccaagcgagc ccccgcggtg cgacgcgagg gctcggacag aaaatcacca gatctcctga
     6181 ggagaggata gctatgtcca ccacaccctt gaaagtcacg acacaggagg cgctcgacaa
     6241 ggcgctcgcg gatggtgtca ccgatctgat cgtcgaggcg cccgactggg cctaccttga
     6301 gatccgccat gacggcctgg tgcggctctt cgggtcgtcc cgcgtcgagg cccgggagtc
     6361 gtcccacgtc gaggcctggg agtcgtcccg cgtcgag
//
LOCUS       DAOMZY010000204         6372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_11416_length_6372_cov_5.654108, whole genome shotgun sequence.
ACCESSION   DAOMZY010000204 DAOMZY010000000
VERSION     DAOMZY010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11416_length_6372_cov_5.654108"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..1274
                     /gene="hemE"
                     /locus_tag="PLK64_11420"
     CDS             102..1274
                     /gene="hemE"
                     /locus_tag="PLK64_11420"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018134455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HPV80401.1"
                     /translation="MPTSEQSPVTDERRAVGAESSVLPPTHPLVTGRTSGSILVRAYR
                     GERTATRPIWMMRQAGRSLPEYRAARGGTAMLDACLTPELAAEITCQPVRRHRVDAGI
                     FFSDIVIPVRLAGVGVDIVPGVGPVIDHPIRSAAAVAALPELDPQALAPITEAVARSV
                     ELLGDTPLIGFAGAPFTVASYLVEGRPSRDHAITKALMHEHPDVWDALLAWVARTTGA
                     FLRAQGLAGASALQLFDSWAGTLSVEDYLAHVQPHSAAVLAQVADLGMPRIHFGTRTR
                     HLLVAMRDAGADVMGVDAQTPLDEAVALLGGRTPVQGNIDPALLDGPWAELDAHVRDV
                     LLRGSTATGHVVNLGHGVPPTTDPDVLTRIVELVHSVPDLLPDLPPGSLPHPDGAP"
     gene            1271..2788
                     /gene="hemG"
                     /locus_tag="PLK64_11425"
     CDS             1271..2788
                     /gene="hemG"
                     /locus_tag="PLK64_11425"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HPV80402.1"
                     /translation="MTAYAGLRLLVVGGGITGLAAAWEATGQGAEVLLVEASDRFGGK
                     LRTEHVDGLAIEAGPDSFVAYRPAALTLVRELGLGEEVVEVRGGRTVHLRTDGRLQPI
                     PDGMGLVLPTKLGPFVRTPVLRWRHKARALLDLVLPRRLGASDVAIGDLLRARLGEGI
                     VQRFADPMLGGIYGATVDELSLDAVLPILREHERTHRSLIIASLASGRAARAAAQSTS
                     GSGGTTRGPSSPFRSLATGLGALPDRLVDALTARGADLRRHTRVDALNPEGERTRVRL
                     SDGTEHVVDGVVLAAGAAASADLVEPYAVSAAAALRQVRHASTAVVTVAWPESAFAAP
                     PTSQGWLEAGDAPVSGVTISSAKWPGRAPAGVVLVRAFVPDRVGRLAEGTDAEILDAV
                     TRHVGSILGAHEPPTLHRVTRWVSVMPKYTVGHLDRVAAVDGGLAGQPSWTVAGSALR
                     GVGIPDCIADGRAAVSRVIGSPRATANASPTITATPATASPTITARQPTPERTPA"
     gene            2785..3552
                     /locus_tag="PLK64_11430"
     CDS             2785..3552
                     /locus_tag="PLK64_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HPV80403.1"
                     /translation="MSTQEQPTATETSAPAAAPTAGLAPATASATKVTAREINETIRY
                     TCWAVFAAAERPDSGTAKRLVEVVAGLAATDVVVRGFYDVSAMRADADVMVWLHGPTP
                     ESIQTAVRAIRRQGFGPAATFVWSGVGMHRPAEFNKGHVPAFMTGAPAQDYLVVYPFV
                     RSLEWYLLPETERRAMLVEHGMMGRAWPQVLTNTVAAFALGDYEWMLALEGPALHDIV
                     DLMRHLRAAEARRHVRVEVPFYTGRLVGIAEAVDALR"
     gene            3549..4571
                     /locus_tag="PLK64_11435"
     CDS             3549..4571
                     /locus_tag="PLK64_11435"
                     /inference="COORDINATES: protein motif:HMM:NF012964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HPV80404.1"
                     /translation="MSLAYDALVLVGFGGPAGPDDVMPFLRRVTAGRPVPQDRLDEVA
                     ARYLELGGSSPIVAQTEALLAALVERLAARGLEVPAYLGHRNSSPTVPDTLARVAADG
                     HTRVLALASSAFPSYSGCRQYRENLGAGLAAAPGLAVRALGPFPELPGLIDAAADLLA
                     SALRSAGPGATVLVTAHSLPLASAATSGPNGGGYVAALEHAAAAVVARATALAGMPRP
                     PAWRLVFQSRSGSPHVPWLEPDVCDAIREIASDATTQTIVVVPLGFLTDHTEVLWDLD
                     REASEAAAAAGLGFVRVPTIGTHPAFLDGLADLLCAHLEGRAPAPAQPAFCSDRCCPS
                     PAQMEG"
     gene            4576..5571
                     /gene="hemB"
                     /locus_tag="PLK64_11440"
     CDS             4576..5571
                     /gene="hemB"
                     /locus_tag="PLK64_11440"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HPV80405.1"
                     /translation="MRPNASFPSARPRRLRTTPAIRRLVAETRLHPAGLIQPLFVAEG
                     LTEPRPLTLMPGVVQHSVESLRVAVAEAATAGVGAVMIFGVPEERDATGSGATDPDGI
                     LAVGVRAAVEAGGGIPVIADLCLDEFTDHGHCGVLDTHGRVDNDATLQAYGDMALVLA
                     DAGAHALGASGMMDGQIGAVREVLDAAGHLDTILLAYAAKYASAFYGPFREAVGSTLT
                     GDRRTYQQDPGNAREALREVRLDLAEGADIVMVKPALAYLDVISRVAAISDVPVAAYL
                     VSGEFAMVEAAAAAGAIDRASAIVEVLTSVTRAGADLVATYWATEVAGWLNGTER"
     gene            5624..>6372
                     /locus_tag="PLK64_11445"
     CDS             5624..>6372
                     /locus_tag="PLK64_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011775401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPV80406.1"
                     /translation="MPGGVSSPVRAFGAVGGTPRFVASAAGGTITDIEGRTYVDLVGG
                     WGPAIVGHAHPAVVEAVQAAVARSASFGAPCLPELELAEAICARVAPVERVRFTSSGT
                     EAVMTAIRIARAATGRRVVLKFAGCYHGHADAFLVAAGSGVATLGLPDSPGVTPAVAA
                     DTIVVPYGDLHAVRAAFAAHPGEIAAALTEAIPANMGVVVPPVGFNAALAQVCRADGA
                     LLVLDEVMTGFRVGPAGRWGLEGAEQGWAPDL"
BASE COUNT          793 a         2416 c         2201 g          962 t
ORIGIN      
        1 tcggccgtgc gtgagcaggg gtggtcggcg gtcacccccg acagcccaga ctcgggcgcc
       61 gttgctcgcg ccctcttccg cgccctcacc aggagccagt gatgccgacg tccgagcagt
      121 cccccgtcac cgacgagcga cgagcggtcg gtgccgagtc gtcggtgttg cctccgacgc
      181 acccgctcgt cacgggccgc acgtcgggct cgatcctcgt gcgcgcctat cgcggtgaac
      241 gcaccgcgac ccggccgatc tggatgatgc gccaggccgg tcgctcgctg cccgagtacc
      301 gcgcggcccg cggcggcacg gcgatgctcg atgcctgtct cacgcccgag ctggccgccg
      361 agatcacctg ccagccggtg cgtcgtcatc gcgtcgacgc gggaatcttc ttctccgaca
      421 tcgtgattcc ggtgcgtttg gctggcgtgg gcgtcgacat cgtccccggc gtgggcccgg
      481 tcatcgacca cccgatccgc tcggccgccg cggtcgccgc cctgccggag ctcgaccccc
      541 aggccctcgc gcccatcacc gaggctgtcg ctcggtcggt tgaactgctc ggcgacaccc
      601 ctctcatcgg gttcgcgggc gcgcccttca ccgtcgcgtc ctatctcgtc gaggggcgac
      661 ccagcaggga ccacgccatc accaaggcgc tcatgcacga gcaccccgac gtgtgggatg
      721 cgctgttggc ctgggtcgct cgcaccaccg gggccttcct tcgcgcccag ggcctcgccg
      781 gggccagcgc cctgcagctg ttcgattcgt gggccggcac gctgagcgtc gaggactacc
      841 tggctcacgt gcaaccgcac tccgccgccg tcctcgccca ggtcgccgac ctcggtatgc
      901 cgcgcatcca cttcggcacg cgcacccgcc acctgctcgt ggccatgcgt gatgccgggg
      961 cagatgtcat gggggtcgat gcgcagactc cgctcgatga ggcagtcgca ctgctcggtg
     1021 ggcgcactcc cgtccagggc aacatcgacc ccgcgctgct cgatggtccg tgggccgaac
     1081 tcgacgccca cgtccgtgac gtcctgctcc ggggctcgac cgcaacgggc catgtcgtca
     1141 acctcggcca cggcgtcccg ccgacgacgg accccgacgt cctcacgcgg atcgtcgagt
     1201 tggtccactc cgtgccagac ctcctgcccg acctcccgcc cggctcgctg ccccaccccg
     1261 acggtgcgcc gtgacggcat acgccgggct gcggttgctc gtcgtgggcg gcgggatcac
     1321 cggcctggct gcggcgtggg aggcgaccgg ccagggcgcg gaggtcctgc ttgtcgaggc
     1381 gagcgaccgc ttcggcggca aactgcggac ggagcacgtc gacggcctgg ccatcgaggc
     1441 cggacccgac tcgttcgtcg cctatcggcc ggcggcgctg accctcgtgc gcgagcttgg
     1501 gctgggcgag gaggtcgtcg aggtccgcgg cggccgcacc gtgcacctgc gcaccgacgg
     1561 acgcctgcag ccgatccctg acgggatggg cctcgtgctg ccgaccaaac tcggcccttt
     1621 cgtccgcacc ccggtcctgc gctggcggca caaggcccgc gcactgcttg acctggtgct
     1681 gccgcgtcgc ctcggcgcga gcgacgtcgc gatcggcgat ctgctgcgcg cgcggcttgg
     1741 cgagggcatc gtgcaacggt tcgccgaccc gatgctcggc ggcatctacg gggcgaccgt
     1801 cgatgagctc agcctcgatg ccgtcctgcc gatcctgcgt gagcacgagc gcacccaccg
     1861 cagcctcatc atcgccagcc tcgcctccgg tcgcgccgcg cgggcggccg ctcagagcac
     1921 cagcggcagt ggagggacga cccgcggacc gtcctcgcct ttccgcagcc tcgccaccgg
     1981 gctcggcgcg ctacccgacc gactcgtcga cgccctcacc gcgcgcggcg ccgacctgcg
     2041 gcggcatacc cgcgtcgacg ccctcaaccc cgagggcgag cggacccgag tgcggctctc
     2101 cgacggcacc gagcacgtcg tcgacggggt cgtcctcgcc gccggtgccg ccgcctcggc
     2161 cgacctggtg gagccgtatg ccgtgtccgc cgccgccgcg ctgcgccagg tgcggcacgc
     2221 ctcgacggcc gtcgtcactg tggcttggcc cgagtcggcc ttcgcggccc caccgacctc
     2281 gcagggctgg ctggaagccg gggacgcccc ggtgagcggc gtgaccatct cgtcggccaa
     2341 gtggccaggg cgggctccgg ctggcgtcgt actcgttcgg gcgttcgtgc ctgaccgggt
     2401 gggccgtctg gcggagggca ccgacgcgga gatcctcgac gcggtgaccc gacacgtcgg
     2461 ctcgatcctc ggagcgcacg agccaccgac cctgcatcgg gtgacccgtt gggtgagcgt
     2521 catgccgaag tacaccgtcg ggcacctcga tcgggtcgcc gccgtcgacg gcggccttgc
     2581 gggacaaccg agttggaccg tcgccgggtc ggcgctgcgc ggggtcggca tacccgattg
     2641 catcgccgac ggacgggccg cggtgagccg tgtcatcggc tcgcccaggg caaccgcaaa
     2701 cgccagcccg acgatcacag ccaccccagc gaccgccagc ccaacgatca cggcccgaca
     2761 gcccacgccc gagaggacac ccgcatgagc acccaggagc agcccaccgc gaccgagacg
     2821 tctgcccctg ccgccgctcc gacagccggg ctggcgccag cgacggcgtc cgcgaccaag
     2881 gtgacggcgc gggagatcaa cgagacgatc cgctacacct gctgggccgt gttcgccgcc
     2941 gccgaacggc ccgactcagg caccgccaag cggctcgtcg aggtggtcgc cggcctcgcc
     3001 gcgacggacg tcgtcgtccg cgggttctac gacgtgtccg ccatgcgcgc cgacgcggac
     3061 gtcatggtgt ggctgcacgg gccaaccccg gagtcgatcc agacggcggt gcgggccatc
     3121 cgccgacaag ggttcggacc ggcggcgacc ttcgtctggt caggcgtcgg gatgcaccgc
     3181 ccggccgagt tcaacaaggg gcatgtgccg gccttcatga ccggcgctcc ggcgcaggac
     3241 tatctcgtcg tctatccgtt cgtccgctcc ctcgagtggt acctccttcc cgagaccgag
     3301 cgccgggcga tgctcgtcga gcacggcatg atgggccgcg cgtggccgca ggtcctcacc
     3361 aacacggtcg cggcgttcgc actcggcgac tacgagtgga tgctggccct cgaaggcccc
     3421 gcgttgcacg acatcgtcga cctcatgcgg cacctccggg cggccgaagc ccgacgccat
     3481 gtccgggtcg aggtcccgtt ctataccggc cgcttggtgg gcatcgccga agccgtcgac
     3541 gcactgcggt gagcctcgcc tacgacgcgc tcgtcctcgt cgggttcggc ggtccggccg
     3601 ggccggacga cgtcatgccg ttcctgcggc gggtgaccgc ggggcgcccg gtgccccagg
     3661 atcggctcga cgaggtcgcc gcccgttatc tcgagctcgg cgggagcagc cccatcgtcg
     3721 cccagaccga agccctgcta gccgctctcg tcgagcggct tgcggcgcgc ggtctggagg
     3781 tgccggcata cctcgggcat cgcaactcca gcccgacggt gccggacacg ctcgcgcgcg
     3841 ttgccgctga tgggcacacc cgggtgctcg cgctcgcatc gagcgccttc ccgtcctatt
     3901 cggggtgtcg gcagtatcgc gagaacctcg gcgccgggtt ggccgccgca ccgggtctcg
     3961 ccgttcgcgc gctcgggccc ttccctgaac tgcccggcct catcgacgcc gctgccgacc
     4021 tgctcgcgag cgcgctccgg tcggcggggc caggggcgac cgtgctcgtc acggcccact
     4081 cgctgccgct cgcctcggcg gccacgtcgg gtccgaacgg aggcgggtac gtcgcggccc
     4141 ttgagcatgc agcggcggcg gttgtcgcac gggccacggc cctcgccggt atgccgcgtc
     4201 cgcccgcctg gcgactggtc ttccagtccc gcagtgggtc accacacgtg ccctggctcg
     4261 agcctgatgt ctgcgatgcg atccgcgaga tcgcttctga cgcaacgact cagaccatcg
     4321 tcgtcgtgcc gttgggcttc ctcaccgacc acaccgaggt gctctgggac ctcgaccgtg
     4381 aggcttccga ggccgccgcg gcggccgggc tcggcttcgt ccgggtgccg acgatcggga
     4441 cccatccggc cttcctcgat gggttggccg acctcctctg cgcccacctt gagggtcgtg
     4501 ccccagcccc ggcgcagccg gccttctgct ccgaccgctg ctgccccagc ccggcgcaga
     4561 tggaaggctg accccatgcg tccgaacgcc tcgttcccca gcgcccgacc tcgccgactg
     4621 cgcacgaccc ctgcgatccg gcggctcgtc gccgagaccc gtttgcatcc ggccgggctc
     4681 atccagccgt tgttcgtcgc cgaaggcctc accgagccgc gcccgctcac gctcatgccg
     4741 ggggtcgtgc agcacagcgt cgagtccttg cgcgtcgccg tcgccgaggc ggccactgcc
     4801 ggggtgggcg cggtgatgat cttcggcgtt ccagaagagc gcgacgcgac cggctccggc
     4861 gccaccgacc cggacgggat ccttgccgtg ggagttcgcg ctgcggtcga ggcggggggc
     4921 gggattcccg tcatcgccga cctgtgcctc gatgagttca ccgaccacgg gcattgcggg
     4981 gtgctcgaca cccacggccg ggtcgacaac gacgcgacgc tgcaggccta cggcgacatg
     5041 gccctcgtcc tcgccgacgc cggggcgcac gccctcgggg ccagcggcat gatggacggg
     5101 cagatcggtg cggttcgcga ggtgctcgac gccgccggac acctcgacac gatcctcctc
     5161 gcgtatgccg cgaagtacgc ctccgccttc tacggcccgt tccgcgaggc ggtcggctcc
     5221 accctgacgg gcgaccgacg gacctaccaa caggacccag gcaacgctcg cgaggcgctg
     5281 cgggaggtcc ggctcgacct ggccgagggt gcggacatcg tcatggtcaa gccggccctg
     5341 gcctatctcg acgtcatctc acgagtcgcc gcgatctcgg acgtgccagt cgcggcatac
     5401 ctggtgtccg gggagttcgc gatggtggag gcggctgcgg ccgcgggggc gatcgaccgc
     5461 gcttcggcca tcgtcgaggt gctcacctcg gtgacgcgag ccggcgccga tctcgtggcg
     5521 acctactggg cgaccgaggt ggccggctgg cttaacggaa cggagcgctg acgtgaccaa
     5581 cggcgtcact tcggaggagt tgttcgcccg ggcccgagcc gtcatgcccg gaggggtgtc
     5641 ctcgccggtg cgcgccttcg gggcggtcgg tgggacgccg cgattcgtcg cctcagccgc
     5701 cggagggacg atcaccgaca tcgaggggcg cacctatgtc gacctcgtcg gcggctgggg
     5761 tccggcgatc gtcggccacg cccatccggc ggtggtcgag gccgtgcagg cggccgtcgc
     5821 gcgctcggcg tccttcggtg caccgtgtct gcccgagctg gaactcgccg aggctatctg
     5881 cgcccgggtt gcacctgtcg agcgggtccg gttcacgtcc tcggggacgg aggcggtcat
     5941 gacggccatt cgcatcgccc gggccgccac cggacgccgc gtcgtgctga agttcgccgg
     6001 ctgctatcac gggcacgccg acgccttcct cgtcgccgcc ggctccgggg tggccacgct
     6061 cggcctcccc gactcccccg gcgtcacgcc ggcggtcgct gccgacacga tcgtcgtccc
     6121 ttatggcgac ctccacgccg ttcgagcggc cttcgccgcc caccccggtg agatcgctgc
     6181 cgcgctcacc gaagcgatcc ccgccaacat gggggtcgtc gtccctccag tcggtttcaa
     6241 cgcggctctg gcgcaggtat gccgcgccga cggcgccctg ctcgtgctcg acgaggtgat
     6301 gaccgggttc cgggtcggac cggccggtcg ctgggggctc gaaggtgccg aacagggttg
     6361 ggcaccagac ct
//
LOCUS       DAOMZY010000205         6369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_11422_length_6369_cov_6.938866, whole genome shotgun sequence.
ACCESSION   DAOMZY010000205 DAOMZY010000000
VERSION     DAOMZY010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11422_length_6369_cov_6.938866"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLK64_11450"
     CDS             <1..229
                     /locus_tag="PLK64_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HPV80407.1"
                     /translation="PAAHHGHREADLAMLALFGSPHLDVTIAAYQDVRPLAEGWRERV
                     ALHQLYPLAVHALLFGGGYAAQTISAARRYA"
     gene            complement(271..1734)
                     /locus_tag="PLK64_11455"
     CDS             complement(271..1734)
                     /locus_tag="PLK64_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HPV80408.1"
                     /translation="MPSRVVSYRPGPPPTLAGEPGSLARRFATIREQLHVPGEFPRDV
                     LAAAADAADLVVLPTVDLTDLGFWTLDPVGSMDLDQAMVLAREGTGYRVRYAIADVPA
                     FVTPGGAIDVEARRRGQTIYLPDTRVPLHPPALSEGAASLLPDQVRPAYVWDLGLDEH
                     ATVTSTHVGRALVRSRARRDYASVQASVDAGTADEQVRLLQEIGQKRAALESARGGAS
                     LPMPEQQVVHEGPGVYRLEFRPPLAAEDWNAQISLMTGMAAAQLMLDAGVGILRTMPA
                     PQRDSVRRFRRQARALGVPWPDGQRYGDFLRGLDRHDPRHLALIHEATSLFRGAGYTP
                     FDGGPPEQQQHAAVAAPYAHVTAPLRRLVDRFGLVVCAAVSAGEPVPTWARESLALLP
                     GLMAEGDRRASAVERACTDAVEAAVMISLVGQSLSGSVVDRNGRGVPVVQLLDPAVVA
                     LAQGTAESGDQVTVRVVGADVAAGRVDLEVVPAPASV"
     gene            1756..2373
                     /locus_tag="PLK64_11460"
     CDS             1756..2373
                     /locus_tag="PLK64_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPV80409.1"
                     /translation="MTDPPNAHLELSLVSHQPVHSPGADTIALALALTRQAPPRCGSV
                     RVVAIDGPSGSGKTTLAAALAQALDAPTVHMDDLFPGWDGLAAAPALLTTQVLEPLAR
                     GERAAYRRWDWIASAWGDVVPVPATDVLVVEGCGASVGPAARYAAVRIWVEAELDVRM
                     ARGIARDGEMYRPHWQRWADQEVTVFGSDHTRTRADIVLDTTHVP"
     gene            2384..2959
                     /locus_tag="PLK64_11465"
     CDS             2384..2959
                     /locus_tag="PLK64_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HPV80410.1"
                     /translation="MEPADMADAVSASMQARTGRTVSEWVDAVTAAGIDPLDQLAVRR
                     WLKDTHGIAQNSQWTIAFAAAEAAGWVRPSADGYTDTLYAGAKAPLRPLHDAVLAVAL
                     ALGPDTEAQGRSTYIPVVRATQFAAIAPGPGGTLRVGLRFRTQVPDDARLEPAKGFAQ
                     ATHWVHLPGASLLADAAALEPLLAAAYLQNG"
     gene            2956..3399
                     /locus_tag="PLK64_11470"
     CDS             2956..3399
                     /locus_tag="PLK64_11470"
                     /inference="COORDINATES: protein motif:HMM:NF014584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HPV80411.1"
                     /translation="MTPSRRRLAARVALGGALGTSARLLVDALVGSPWGTWDAGVAVA
                     NVTGAFFLGIIALYPFRRRHPHEVRAFAGTGVMGAYTTFSALNLAMVDGSPRPEAVLG
                     LLVSIGGGCVAAWLGLRVGLSARRAVEAPGEVPGSVDPRDGEEDL"
     gene            3396..3821
                     /locus_tag="PLK64_11475"
     CDS             3396..3821
                     /locus_tag="PLK64_11475"
                     /inference="COORDINATES: protein motif:HMM:NF014584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HPV80412.1"
                     /translation="MSIGGYAVWTWLGAVAAGSAGAVLRWASVVWLHGILSRRSGATD
                     PFPLGVLVANTVASFLAGVAVAVAPVVGPQWHLVLVGGLCGGLSTLSTLAVDTVAMWQ
                     RGERWGAVGNLGATLALGLGAAMIGAGVVGSAVAAALSR"
     gene            complement(3881..4363)
                     /locus_tag="PLK64_11480"
     CDS             complement(3881..4363)
                     /locus_tag="PLK64_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPV80413.1"
                     /translation="MSEVSHPTGRRGDDDPFAPFVPRRGRIVAISLAVAAIALFTFIG
                     VFIPATAIHDFQIFDRVLFASIGWILAALFFRYATIKAVPTREGLTVRNLFLTRTVTW
                     PQVMGVQFGQGMPWPTLELNDTDTLAVMAIQRSDGPRSLQESARLNALVTGLGEARER
                     "
     gene            complement(4392..5237)
                     /gene="hisG"
                     /locus_tag="PLK64_11485"
     CDS             complement(4392..5237)
                     /gene="hisG"
                     /locus_tag="PLK64_11485"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPV80414.1"
                     /translation="MLRIAVPNKGSLAEPAADMLRQAGYKVRRDLKELIVTDPDNEVE
                     LFYLRPRDIAVYVGSGTVDAGITGRDLLLDSGSDAVEVMALGFGKSTFRYAARAGSMS
                     DVADIAGRRVATSYPGLVQGHLAGAGVSAAVVRLDGAVETAITLGVADVIADVVETGA
                     TLRSQGLAVFGDPILTSEAVLIRRAEVSEDNGVGVLRRRLLGVVTARDYVLLDYDVPA
                     AAVEAACQITPGLESPTVSPLHDPAWVAVRAMVPRKGTNRVMDELFDLGARGILVTHI
                     AACRL"
     gene            complement(5259..5522)
                     /locus_tag="PLK64_11490"
     CDS             complement(5259..5522)
                     /locus_tag="PLK64_11490"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HPV80415.1"
                     /translation="MKTFDGLFAELADKAATRPAGSGTVAQLDAGVHAIAKKIVEEAA
                     EVWMAAEHEGPERAAEEISQLLYHLQVLMLASGLTLDDVYAHL"
     gene            complement(5618..6031)
                     /locus_tag="PLK64_11495"
     CDS             complement(5618..6031)
                     /locus_tag="PLK64_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HPV80416.1"
                     /translation="MDTAPTPGSMPLAAAYSRVAVTEAAVTLLRKLTDQHGPLMFHQS
                     GGCCDGSSPMCYPDGEFILGDSDVHLGDLGIGLDRAVPMWMSASQFAYWQHTHLTVDV
                     VPGRGAGFSVEAPEGVRFLIRSRLFTDDELDALES"
     gene            complement(6036..>6369)
                     /locus_tag="PLK64_11500"
     CDS             complement(6036..>6369)
                     /locus_tag="PLK64_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPV80417.1"
                     /translation="RIFQEEIFGPVVSVTRFEDEADALKIANDTLYGLGAGVWSRDGG
                     RAFRAGRSIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRENHKMMLDHYQQTKNLLVSY
                     SPNALGFF"
BASE COUNT          956 a         2333 c         2185 g          895 t
ORIGIN      
        1 ccccgccgcc caccatggtc accgcgaggc cgacctggcg atgctcgcgc tgttcgggtc
       61 gccgcacctt gacgtgacga tcgcggcata ccaggacgtg cggccgctcg ccgaagggtg
      121 gcgtgagcgg gtcgccctgc atcagctcta tccgctggct gtccacgccc tgctcttcgg
      181 cggcgggtat gccgcgcaga ccatctcggc tgcgcgccgc tacgcctgag cctcgccccg
      241 actagaccga cgcgacgaga ccgaccccgg ctagaccgac gcgggcgccg ggacgacctc
      301 caggtccacc cgtccggccg cgacgtcggc accgaccacc cggaccgtga cctgatcacc
      361 cgactcggcc gtcccctgcg cgagcgccac gaccgccggg tcgagcaact gcacgaccgg
      421 caccccgcga ccgttacggt ccaccacgct gccagacaac gactggccga cgagcgagat
      481 catcacggcg gcctccaccg cgtcggtgca ggcccgctcg accgcggacg cgcgccggtc
      541 accctcggcc atcaggccgg gcagcagcgc gagcgactct cgcgcccacg tcggcaccgg
      601 ctcccccgcg ctcaccgcgg cgcagaccac caacccgaac cggtcgacga gccggcgcag
      661 cggagccgtc acgtgggcat acggcgcagc cacggcggcg tgctgctgct gctccggagg
      721 cccgccgtcg aagggcgtat agcccgctcc ccggaagagc gaggtcgcct cgtggatgag
      781 cgcgaggtgg cgcgggtcat gacggtccag gccgcgcagg aagtcgccgt agcgctggcc
      841 atccggccac ggcaccccga gcgcccgcgc ttgacgacgg aagcgacgca ccgaatcccg
      901 ttgcggcgca ggcatggtcc gcaggatccc cacaccggcg tcgagcatga gttgagctgc
      961 ggccattccg gtcatcaggg agatctgggc gttccagtcc tcggccgcca agggcgggcg
     1021 gaactccaaa cggtagaccc caggcccctc gtggacgacc tgttgctcgg gcatcggcaa
     1081 ggaggccccg ccgcgcgccg actccaacgc cgcccgcttc tgcccgatct cctgcagcaa
     1141 cctcacctgc tcatccgcgg tcccggcatc gaccgacgcc tggacgctcg cgtagtcgcg
     1201 tcgggcccga ctgcgcacca gggcccgccc gacgtgcgtc gacgtcaccg ttgcgtgctc
     1261 gtccagaccc agatcccaca cgtatgccgg tcgcacctga tccgggagca ggctcgccgc
     1321 tccctcggac agggccggag ggtgcagcgg cacccgggtg tccggcaggt agatggtctg
     1381 cccccggcgc cgggcctcca cgtcgatcgc tccgcccggg gtgacgaacg ccggcacatc
     1441 ggcgatcgcg tagcggaccc gataccccgt gccctcgcgc gccagcacca tcgcctggtc
     1501 gaggtccatc gacccgaccg ggtcgagggt ccagaaaccc aggtcggtga ggtcgaccgt
     1561 cggcaggacg accaggtcgg ctgcgtcagc ggctgcagcg agcacgtccc gcgggaactc
     1621 gccaggcaca tgcaactgct cgcggatcgt ggcgaacctg cgggccagcg accccggctc
     1681 gccggcaagg gtcggcggcg gacctgggcg atagctgacg acacggctgg gcatgggcac
     1741 accctaggac tcctggtgac cgacccgccc aacgcccacc tagagttgtc gctcgtgtcc
     1801 catcagcccg tccactcccc cggcgcggac accatcgcgt tggcgttggc cctgacccgt
     1861 caggcaccgc cacgatgtgg gtcggtccgg gtcgtcgcca tcgacgggcc tagcgggtcg
     1921 ggcaagacca ccctggcggc cgcgctggcg caggccctgg acgcaccgac ggtgcacatg
     1981 gacgacctct tcccggggtg ggacggcctg gccgccgcac cggccctcct caccacgcag
     2041 gtcctcgagc cgctcgcacg gggtgagcgg gcggcatacc ggcgatggga ctggatcgcg
     2101 agcgcatggg gcgatgtcgt gccggtcccg gccaccgacg tgctcgtcgt cgaaggctgc
     2161 ggcgcgagcg tcggcccggc cgcccggtat gccgcggtgc ggatctgggt cgaggcagag
     2221 ctcgacgtgc gcatggcccg cggcatcgcc cgtgacggcg agatgtaccg cccgcactgg
     2281 caacggtggg ccgaccaaga ggtgacggtc ttcgggtccg atcacacccg aacgcgagcc
     2341 gacatcgtcc tcgacaccac ccatgtgccc taacgtttct cgcatggaac ccgccgacat
     2401 ggccgatgcc gtctcggcct cgatgcaggc acgcaccggc cggaccgtgt cggaatgggt
     2461 cgacgccgtc accgccgcag ggatcgaccc cctcgaccaa ctcgccgtcc gacgctggct
     2521 gaaggacacc cacggcatcg cgcagaacag ccagtggacc atcgcgttcg cggctgccga
     2581 ggcggccggg tgggtgcgac cgagcgccga cggctacacc gacaccctgt atgccggtgc
     2641 caaggccccg ctgcggccgt tgcacgacgc ggtcctcgcg gtcgccctcg cgctcggccc
     2701 cgacaccgag gcacagggtc gatcgaccta catcccggtc gtccgggcca cccagttcgc
     2761 cgccatcgcg cccgggcccg gcggcaccct gcgggtcggc ctgcgcttcc gcacccaggt
     2821 ccccgacgac gcccgcctcg agccggccaa gggtttcgcc caggccactc actgggtgca
     2881 cctgccgggc gccagcctcc tggccgacgc cgccgccctc gaacccttgc tcgcggcggc
     2941 atacctgcag aacggatgac cccctcccgc cgccgcctgg ccgcccgggt cgccctcggc
     3001 ggcgcactcg gcacgtccgc ccggctcctc gtcgacgcac tcgtcggctc gccgtggggc
     3061 acgtgggacg ccggggtcgc cgtcgccaac gtcaccgggg cgttcttcct cggaatcatc
     3121 gccctctatc ccttccgccg ccggcacccg cacgaggtgc gtgccttcgc cggcaccggc
     3181 gtcatgggtg cctacacgac gttctccgcc ctcaacctcg cgatggtcga cggctcacca
     3241 cggcccgagg ccgtgctcgg cctgctcgtc agcatcggcg gcgggtgcgt cgcggcctgg
     3301 ttgggtctgc gggtggggct gtccgcgcgc cgcgcggtcg aggcgcccgg cgaggtcccc
     3361 gggtccgtcg acccccgcga cggcgaggag gacctgtgag catcggcggg tatgccgtgt
     3421 ggacctggct cggggcggtc gccgccggtt cggccggggc cgtgcttcgg tgggccagcg
     3481 tcgtctggct gcacgggatc ctctcccggc gcagcggcgc caccgatccc ttcccgcttg
     3541 gcgtcctcgt ggccaacacc gtggcctcgt tccttgccgg ggtggcggtg gcagtagccc
     3601 ccgtggtcgg accccaatgg cacttggtcc tcgtcggcgg gctctgtggc gggctctcaa
     3661 ccctctcgac cctggcggtc gacacggtgg cgatgtggca acgcggcgaa cgttggggcg
     3721 cggtcggcaa cctcggcgcc accctcgccc tcgggctcgg tgccgcgatg atcggagctg
     3781 gcgtcgtcgg cagcgctgtg gctgcggccc tgagccgtta gatcccgcgc cgatagatcc
     3841 cgcgccaata ggtccggcgc cgatagatcc agccggacag tcagcgctcg cgggcctctc
     3901 ccaggccggt caccagggcg ttgagccgag ccgactcctg cagcgaccga ggcccatccg
     3961 agcgttggat cgccatgacg gccaaggtgt cagtgtcgtt gagttcaagg gtcggccaag
     4021 gcatcccctg cccgaactga acccccatga cctgcggcca ggtgaccgtc ctggtgagga
     4081 agaggttgcg gacggtgagc ccctcgcggg tcggcaccgc cttgatcgtt gcgtagcgga
     4141 agaagagggc cgcgaggatc caaccgatcg aggcgaagag cacccggtcg aagatctgga
     4201 agtcgtggat cgcggtcgcc gggatgaaga ccccgatgaa ggtgaacagc gcgatggcgg
     4261 ccaccgccag gctgatcgcg acgattcggc cgcggcgagg aacgaacggc gcgaagggat
     4321 cgtcgtcgcc ccgacgcccg gtgggatgac tcacttcgct catgtcgatc tcctccggaa
     4381 cgccgagcgg gtcacagtcg acaggctgcg atgtgagtga cgaggatccc tcgggcaccg
     4441 aggtcgaaca actcgtccat cacccggttg gtccccttgc gcggcaccat cgcccgcaca
     4501 gccacccagg ccgggtcgtg caacggcgaa acggtcggcg actccagacc gggggtgatc
     4561 tggcaggccg cctcgaccgc ggcagcgggc acgtcgtagt cgagcaggac gtagtcacgt
     4621 gcagtgacga cgccgagcag gcgacgccgc agcaccccga cgccgttgtc ctcggatacc
     4681 tcggcacggc ggatgagcac cgcttccgac gtgaggatgg ggtcgccgaa gaccgccaga
     4741 ccctggctgc gcaaggtcgc ccccgtctcc acgacatcgg cgatgacgtc ggccacgccg
     4801 agggtgatcg ccgtctcgac ggctccgtcg agacgcacga ccgcagcgct cacgccagcc
     4861 ccggccaggt gcccctgcac gagccccggg tagctcgtcg ccacccgccg acccgcgatg
     4921 tcggcgacgt cgctcatcga ccccgcgcgg gcggcatacc ggaaggtcga cttgccgaaa
     4981 ccgagagcca tgacctcgac ggcatcgctg cccgagtcga gcagcaggtc gcgaccggtg
     5041 atcccggcgt cgaccgtgcc ggacccgaca tagacggcga tgtcacgcgg tcgaaggtag
     5101 aagagttcga cctcgttgtc ggggtcggtg acgatgagct ccttgaggtc ccggcggacc
     5161 ttgtatccgg cctgccggag catgtcggcg gccggctcgg ccagagagcc cttgttgggg
     5221 acggcaatgc gcagcatgag agatccaggg ggaagcggtc agaggtgggc gtagacgtcg
     5281 tcgagggtga ggcccgaggc gagcatcagc acctgcaggt ggtagagcag ctggctgatc
     5341 tcctctgcgg cccgctcggg gccttcgtgc tcggccgcca tccacacctc ggccgcctcc
     5401 tcgacgatct tcttggcgat ggcatgaacg ccggcatcga gctgcgcgac cgtgccggag
     5461 ccggccggtc gggtggcggc cttgtcggcc agctcggcga acagcccatc gaacgtcttc
     5521 acgaggaatc agcctaccca ggcgcaggta tgccgcccgc ccaccttcca gcggacggac
     5581 ggcatacctg atgggtctgg ccacccgccg atcgggctca gctctcgagc gcgtcgagtt
     5641 cgtcgtcggt gaacagccgg gacctgatga ggaaccgcac cccctccggc gcctcgacgg
     5701 agaacccggc cccgcggccg ggaacgacat cgacagtgag gtgggtgtgc tgccagtagg
     5761 cgaactgact ggccgacatc cacatcggca cggctcggtc cagcccgatg ccgagatcac
     5821 ccaggtggac gtccgagtcg ccgaggatga actcgccgtc ggggtaacac atcggagacg
     5881 agccgtcgca gcagccaccg gactggtgga acatcaacgg cccgtgctgg tcggtgagct
     5941 tgcgcagcag ggtcaccgct gcttcggtca ccgccacgcg ggagtatgcc gccgcgagcg
     6001 gcatactccc tggcgttggc gcagtatcca tcggtttaga agaagccgag ggcattgggc
     6061 gagtatgaca ccagcaggtt cttggtctgc tggtagtggt cgagcatcat cttgtggttc
     6121 tcgcggccga tgcccgactg cttgtagcca ccgaaggcgg cgtgggccgg gtaggcgtga
     6181 tagcagttgg tccacacccg gccggcctgg atcgagcgcc cggcgcggaa ggcccgaccc
     6241 ccgtcgcggc tccacacgcc ggcaccgagg ccatagaggg tgtcgttggc gatcttgagc
     6301 gcgtcagcct cgtcctcgaa ccgggtcacc gagacgaccg ggccgaagat ctcctcctgg
     6361 aagatgcgc
//
LOCUS       DAOMZY010000206         6363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_11447_length_6363_cov_7.428345, whole genome shotgun sequence.
ACCESSION   DAOMZY010000206 DAOMZY010000000
VERSION     DAOMZY010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11447_length_6363_cov_7.428345"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PLK64_11505"
     CDS             complement(<1..251)
                     /locus_tag="PLK64_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HPV80418.1"
                     /translation="MLISSLVTNPLWDFGYTFQIMQQKSVIQGLWLGTLLGTVGSMII
                     GVVLGVILAIMRLSDNPVLRGAAFVYIWFFRSMPRYVLLA"
     gene            complement(356..1285)
                     /locus_tag="PLK64_11510"
     CDS             complement(356..1285)
                     /locus_tag="PLK64_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPV80419.1"
                     /translation="MARRHTLTYLAGIAVAGLALSACGSSSLTPTATTAASKAATAAA
                     TSAATAASAIDPALVAKIPAKIKSAGKIVIGTDATYQPNEYLDTDGKTVIGMDVELFD
                     AAMAKFGLKTEWVPSAFDSIITGVGSGKFDIGVSSFTVNDERKQAATMVSYFKAGTQW
                     VVAKGNPKNIDPNNACGLKVAVQKGTVQVDDLTARSKKCTDAGKAEITPLVDPDQAKV
                     TAMVQSGKADAMMVDLPPAIAAVDLTGGTVQLLGEQYDSAPYGYVLPKAETDFAQAIV
                     DSLKKLQADGSYTKILTKWKCQGGAITDFAVNP"
     gene            1435..2277
                     /locus_tag="PLK64_11515"
     CDS             1435..2277
                     /locus_tag="PLK64_11515"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HPV80420.1"
                     /translation="MNVEPWSDVHEGDDLLVIVRGLQYLLRGHGHVIVADGLFGPATR
                     AAMTAFQGAHGIPASGVVDLPTWLALVRTTRLGSSGEFVKAVQSFQLPRWVDDDPLGV
                     DGNFGPLTEERVRWFQKSWGLSQDGAAGRETWSFLQALRPGVDLWPLVKPGSTQATNH
                     RVLAIQHLLRHHGASIVADGSYGPATGEAVRQWQLTQRATYISTTAGQLDWPGLIVSA
                     HLGDHGEHVKAIQTLLSGLVVDGNFGPLTDAAVRGLQGVFLPPADGIVGPDTWHALVV
                     PLFD"
     gene            complement(2306..2773)
                     /gene="paaI"
                     /locus_tag="PLK64_11520"
     CDS             complement(2306..2773)
                     /gene="paaI"
                     /locus_tag="PLK64_11520"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HPV80421.1"
                     /translation="MSTPAAKRTPPPLPGFDLGFLEDQWDDDQASRHIGVEIQLLELD
                     HAVFSLKITDHLVNGHGIVHGGYIFTLADTTFAYACNSRGLTTVAAGVDITFVQAAYL
                     GDVLIADARVRANWGRSGITDVTVIRESDGALVAEFRGRSRQIPPKATPTALA"
     gene            complement(2770..3951)
                     /locus_tag="PLK64_11525"
     CDS             complement(2770..3951)
                     /locus_tag="PLK64_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HPV80422.1"
                     /translation="MGADVDKLGPVGVIGAGAMGSGIAQVAASAGHPVFLCDAFEGAA
                     ERAQTRIAADLAKRAAQGRITAEDAAAIAARITPVAAATHLPACIVVIEAIAEDLDAK
                     SALFEELGRTQPATTILATNTSSLSIDAIAPAAAHPGRVLGLHFFNPPPAMKLVEVIR
                     GETTTQAVLEAATALVVSWGKVAVRCASTPGFIVNRVARPFYGEAQRMVQEGVADAAT
                     IDACLRSAGFRMGPLELTDLIGQDVNLAVGTSVWNQTSRDPRYAPTPFQQGLVAAGHL
                     GRKSGQGVYTYAADGTSPNNVPDEARVAELLASGVITNPVARTLAMLVNEAVDLVDRG
                     EAGPADVDLAMTLGTGYPKGPIAWGREIGFLKVRDQLLELAAAFPGGRYRPSPALEKI
                     S"
     gene            4050..5759
                     /gene="paaN"
                     /locus_tag="PLK64_11530"
     CDS             4050..5759
                     /gene="paaN"
                     /locus_tag="PLK64_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaN"
                     /protein_id="HPV80423.1"
                     /translation="MTTLATTPAHPLVEKYQTVLDEACEALATRSYYSRYPESPSPKV
                     YGENAAADGLAAYQQHLDAPYAALASQPSDGTEVGSEVSPYGPVLGVRYPRLDLDAAL
                     AAATAAIPAWRDAGARVRAAVCVEMIERINARSFEMANAVMHTSGQPFVMSFQAGGPH
                     AQDRALEAVIAGMVEMERIPASAIWEKPGRDAPLRMRKDYQVVPRGVALVIGCNTFPT
                     WNGYPGLFASLVTGNPVIIKPHPRAALPLAITVEIARDVLRENGFDPALVQLAAENDG
                     DGLAKTLAERPEIAIIDYTGGPFFGAWLEQHGAASGQLVFTEKAGVNTVVIDSTDNLK
                     GMLGNLAFSFTLFSGQMCTTPQNVYVQAEVPTDEGVLTFDEIADRIGAAVARFTADDA
                     KAVEMLGATVNPQVRANAAALEQIASDADGRVVLASREVAHPAYPDAVCRAPGLVALD
                     ASAEATYTKEWFGPVAFVIKTASTEASLERLVSTIREHGAMTAAVYSSDEAVLEQARA
                     AASAAGVALSENLTSSVFVNQTAAFSDYHGTGANPAANASYTDAAFVANRFRVITSRR
                     HIA"
     gene            complement(5795..6181)
                     /locus_tag="PLK64_11535"
     CDS             complement(5795..6181)
                     /locus_tag="PLK64_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HPV80424.1"
                     /translation="MKNIAIKTLVNGLALWLAAIVVDGIEFGRGAEWTETLKTVVLVA
                     LVFGLVNAVVKPILKFFATPLIWLTLGVGLLVVNALMLQLTSWLAERLTLAFHVTEFF
                     WDAVIGALIITFVSMVLHAVIPDPKN"
BASE COUNT          975 a         2119 c         2245 g         1024 t
ORIGIN      
        1 gcgaggagta cgtagcgcgg catggagcgg aagaaccaga tgtagacgaa cgccgctccg
       61 cgtagcaccg ggttgtccga gagtcgcatg atcgcgagga tcacgccaag tacgacgccg
      121 atgatcatcg aaccgacggt gcccagcagc gtgccgagcc acaaaccctg gatgaccgac
      181 ttctgctgca tgatctggaa ggtgtacccg aagtcccaca gcgggttggt caccaacgag
      241 ctgatgagca tcgcgacgac gatcacgatg atggctacgc caagccaacg gcccgggtgc
      301 cggaccggcc gggcgtgaat cacgcccggc cggtccgtcg attgagtcgt caccgtcacg
      361 ggttcactgc gaagtcggtg atggctccac cctggcactt ccacttggtc aggatcttgg
      421 tgtagctacc gtcggcctgg agcttcttga gagagtcgac gatggcctga gcgaagtcgg
      481 tctcggcctt gggcaggacg tagccgtagg gggccgagtc gtactgctcg ccgagcaact
      541 gaaccgtgcc gccggtgagg tcgactgcgg cgatggccgg cgggaggtcg accatcatgg
      601 cgtcggcctt gcccgactgc accatcgcgg tcaccttggc ctggtcgggg tcaaccaggg
      661 gggtgatttc agccttgccg gcgtcggtgc acttcttgct gcgggcggtc aggtcgtcga
      721 cctgcaccgt gcccttctgg accgcgacct tgagcccaca ggcgttgttg gggtcaatgt
      781 tcttggggtt gcccttggcc acgacccact gagtaccagc cttgaagtag ctcaccatgg
      841 tggcggcttg cttgcgctcg tcgttgaccg tgaaggacga gacaccgatg tcgaacttcc
      901 cgctgccgac gccggtgatg atcgagtcga acgccgaggg cacccattcg gtcttcaacc
      961 cgaacttggc catggcggcg tcgaagagct cgacgtccat gccgatgacc gtcttgccgt
     1021 cggtgtcgag gtactcgttg ggctggtagg tcgcgtccgt cccgatgacg atctttccgg
     1081 ccgacttgat cttggccggg atcttcgcga cgagcgccgg gtcgatggcg ctggccgcgg
     1141 tggcggcgga cgttgccgcg gcggtcgcgg ccttgctcgc agcagtcgtt gcggtggggg
     1201 tcagtgagct ggagccacaa gccgacagtg cgagtcccgc aactgcgatg ccagccaggt
     1261 aggtcagggt gtgacgacgt gccatggatg tttccttccg aggtgatccg cttgcacggc
     1321 atgtcatgcc ctgcattttc ggcatcctgc cactcgacct gcagatctgg ctatggacgc
     1381 ggctgcacgc ggcgacggcg tagcctgact gttacccatc gggagggact cgggatgaac
     1441 gttgaaccat ggtcggacgt gcacgagggc gacgatctgc tcgtcatcgt gcgaggcctt
     1501 caatacctct tgcgcggcca cggccacgtc atcgtcgccg acgggctgtt cggcccggcc
     1561 acccgggcgg cgatgaccgc cttccaaggg gcgcacggca taccggcatc gggagtcgtc
     1621 gacctgccga cttggctggc cctcgtgcgc accacccgcc tcggcagttc gggcgagttc
     1681 gtcaaggcgg tgcagagctt ccaactgccg cggtgggtcg acgacgaccc gttgggggtc
     1741 gacggcaatt tcgggccgct gaccgaggag cgcgtccggt ggttccagaa gtcatggggg
     1801 ctgagccagg acggcgccgc cggccgggag acctggtcgt tcctgcaggc gctgcgcccc
     1861 ggcgtggacc tctggccgct ggtcaagccc ggctcgacgc aggcgaccaa ccaccgggtc
     1921 ctggcgatcc aacacctgct gcgccatcac ggggcgagca tcgtcgccga cgggagttac
     1981 ggtccggcga cgggtgaggc ggtgcgccag tggcagctca cccagcgcgc gacctacatc
     2041 agcacgactg ccgggcaact cgattggccg gggctcatcg tctccgccca cctgggcgat
     2101 cacggcgagc acgtcaaggc gatccagacg ctgctcagcg ggctggtcgt cgacggcaac
     2161 ttcggtccgt tgacggacgc cgccgtgcgc gggttgcagg gggtcttcct cccgcccgcc
     2221 gacgggatcg tcggccccga cacctggcac gcgctcgtcg tcccgctctt cgactgaacg
     2281 gactctggtc tccagcgggg cggcgtcagg ccagcgccgt cggggtcgct ttgggcggga
     2341 tctgtcgcga gcggccgcgg aactcggcca cgagcgcgcc gtcggactcg cggatcaccg
     2401 tgacgtcggt gatgccgcta cggccccagt tggcgcgcac gcgggcgtcc gcgatgagga
     2461 cgtcgccgag gtatgccgcc tgcacgaagg tgatgtcgac gccggccgcc acggtcgtga
     2521 ggccgcggga gttgcaggcg taggcgaagg tcgtgtcggc gagggtgaag atgtagccgc
     2581 cgtggacgat gccgtgcccg ttgacgaggt gatcggtgat cttgagcgag aagaccgcgt
     2641 ggtcaagctc aaggagttgg atctcgacgc cgatgtgccg ggaggcctgg tcgtcgtccc
     2701 actggtcctc aaggaagccg aggtcaaagc ccggcagcgg gggcggcgtg cgcttggcgg
     2761 caggggtgct cacgagatct tctccagcgc ggggctcggc cggtagcggc cgccggggaa
     2821 ggccgcggcc aactcaagca actggtcgcg gaccttaagg aagccgatct cgcggcccca
     2881 ggcaatcgga cccttggggt agccggtgcc caacgtcatc gccaggtcga cgtcggcggg
     2941 gccggcctcg ccccggtcga cgaggtcgac ggcctcgttg acgagcatcg ccagcgtgcg
     3001 cgcgaccggg ttggtgatca ccccgctggc cagcagttca gcgacgcgtg cctcgtcggg
     3061 gacgttgttg ggggaggtgc cgtccgcggc atacgtgtag acgccctgac cggacttgcg
     3121 cccgaggtga cctgcggcga cgagtccctg ttggaaaggc gttggggcat aacgggggtc
     3181 gcgggaggtc tggttccaca cggaggtgcc gacggcgagg ttgacgtcct ggccgatgag
     3241 gtcggtgagc tccagcggcc ccatccggaa gcctgccgag cgcaggcacg cgtcgatggt
     3301 ggcggcgtcg gcgacgccct cctggaccat ccgctgcgcc tctccgtaga acgggcgcgc
     3361 cacgcggttg acgatgaacc ccggcgtcga cgcgcagcgc accgcgacct tgccccacga
     3421 gaccaccagc gcggtcgcgg cttcgaggac ggcctgggtg gtcgtctcgc cgcggatgac
     3481 ctcgacgagt ttcatggccg gcggcgggtt gaagaagtgc aggccgagca cccggcccgg
     3541 gtgcgccgca gccggggcga tggcgtcgat cgacaggctc gaggtgttgg tggcgaggat
     3601 cgtcgtggcc ggctgggtgc gcccaagctc ctcgaagagt gccgacttgg cgtcgaggtc
     3661 ctcggcgatc gcctcgatga cgacgatgca ggcgggcaga tgggtggcgg cggccaccgg
     3721 agtgatacgt gcggcgatgg cggcggcgtc ctcggcggtg atccgcccct gggcggcccg
     3781 cttggccagg tcggcggcga tccgcgtttg ggcccgctcg gcggccccct cgaacgcgtc
     3841 gcagaggaac accgggtgac ccgccgaggc agccacctgt gcgattccgg agcccatggc
     3901 ccccgcgccg atcactccga ccggccccaa cttgtcgacg tcagcaccca tggggccatc
     3961 ttggtgtacc cccgggcggc tggccacgtg aggttgccga ccacttgtcg acgtgacgcg
     4021 ctcttgcgct cgcagatatc atcaagattg tgacgacttt ggcaacgacg cccgcccacc
     4081 ccctcgtcga gaagtaccag acggtcctcg acgaggcctg tgaggccttg gccacgcgca
     4141 gctactactc ccgctacccc gagtccccgt cgccgaaggt ctacggcgag aacgccgcgg
     4201 ccgacgggct cgcggcatac cagcagcacc tcgatgcccc gtatgccgcg ctggcgagcc
     4261 agccgagcga cggcaccgag gtgggcagcg aggtctcccc ctacgggccg gtgctcgggg
     4321 tgcgctaccc gcggctggac ctcgacgcgg cgctcgcggc ggccactgcg gccatccccg
     4381 cgtggcgcga cgccggcgct cgggtgcgcg cggccgtgtg cgtcgagatg atcgagcgga
     4441 tcaatgcgcg gtccttcgag atggccaatg cggtcatgca cacctcgggc cagccgttcg
     4501 tcatgtcgtt ccaggccggt gggccgcacg cccaggaccg cgcgctcgag gcggtcatcg
     4561 ccgggatggt cgagatggag cggatccccg cgtcggcgat ctgggagaag cccgggcgtg
     4621 acgccccgct gcggatgcgc aaggactacc aggtcgtgcc gcgtggggtc gcgctggtca
     4681 tcgggtgcaa caccttcccg acgtggaacg gctatccggg cctgttcgct tcgctcgtca
     4741 cgggcaaccc ggtcatcatc aagccgcacc cgcgcgctgc gctgccgctg gccatcaccg
     4801 tcgagatcgc ccgggatgtc ctgcgcgaga acgggtttga cccggcgctc gtgcagcttg
     4861 ccgctgagaa cgacggcgac ggcctggcca agaccctcgc cgagcggccc gagatcgcga
     4921 tcatcgacta cacgggcggg ccgttctttg gcgcgtggct cgagcagcat ggcgcggcca
     4981 gcggccagct ggtcttcacc gagaaggccg gcgtcaacac ggtcgtcatc gactcgaccg
     5041 acaacctcaa ggggatgctc ggcaacctcg ccttctcgtt cacgctcttc tccgggcaga
     5101 tgtgcacgac cccgcagaac gtctatgtcc aggccgaggt cccgaccgac gagggcgtgc
     5161 tgaccttcga cgagatcgcc gaccggatcg gcgcggcagt cgcgcgattc acggctgacg
     5221 acgccaaggc cgtcgagatg ctcggcgcga cggtcaaccc gcaggtgcgg gccaacgccg
     5281 cggctctcga gcagatcgcg tccgacgctg acggtcgcgt tgtcctcgcc tcccgcgaag
     5341 ttgcgcaccc ggcatacccc gatgccgtat gccgcgcgcc cggcctcgtc gcgctggacg
     5401 cctccgcgga ggcgacctac acgaaggaat ggttcgggcc ggtggccttc gtcatcaaga
     5461 cggcctcgac cgaggcgtcg ctggagcggc tcgtctcgac gatccgtgag cacggcgcga
     5521 tgacggcggc ggtctattcg tccgacgagg ccgtgttgga gcaggcgcgc gcggcggcat
     5581 cagcggcggg cgtggcgttg tcggagaacc tcaccagctc ggtgttcgtc aaccagacgg
     5641 cggcgttctc ggactaccac ggcacgggcg ccaacccggc ggcgaacgcg tcttacacgg
     5701 atgcggcgtt cgtcgcgaac cggttccggg tcatcacgag ccggcgccac atcgcctgat
     5761 tgggttcggt cgtgcggtca cccgaccccg aggatcagtt cttggggtcg gggatgactg
     5821 cgtgcagcac catcgacacg aacgtgatga tgagcgcgcc gatcacggca tcccagaaga
     5881 actcggtgac gtggaaggcg agggtcaacc tctcggcgag ccacgaggtc aactgcagca
     5941 tgagcgcgtt gacgaccagc aggcccacgc ccagggtcag ccagatgagg ggcgtcgcga
     6001 agaacttgag aatcggcttg acgaccgcgt tgaccaggcc gaacaccagc gcgacgagga
     6061 cgaccgtctt gagcgtctcc gtccactccg ccccgcgacc gaactcgatc ccgtcgacga
     6121 cgatcgccgc caaccacagc gcgagcccgt tgacgagggt cttgatcgcg atgttcttca
     6181 tcgtcttcat ggcgtcatgg tgccaggtca cgccggcggg ccggctcccg cagctgctcc
     6241 ggtcgcgccg ggtcgtagat ttgtcgccat gagcactgct gccgacggat cccccgcccc
     6301 gaccctgcgc gccgccctcg acgcagtccc ggcctacatc cccgggaagc ccgcgagcgc
     6361 gcc
//
LOCUS       DAOMZY010000207         6352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_11485_length_6352_cov_4.821820, whole genome shotgun sequence.
ACCESSION   DAOMZY010000207 DAOMZY010000000
VERSION     DAOMZY010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11485_length_6352_cov_4.821820"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="PLK64_11540"
     CDS             complement(<1..765)
                     /locus_tag="PLK64_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HPV80425.1"
                     /translation="MRRRPRSTLAGQLFALQLAIIAVVLVAVAAVSLAQSAATFERVE
                     GRRVAALAEQVASSPLVRASLAETETRTGLATLAQSLVTQTSVSSVSFAETDLDVRVS
                     TDPSLEGAPMPLGDPRVADGASWSGTLEIDGVAHLVSQVPVLGESEDAVGAHLGSAMV
                     AERVPSTWERLQGTSSYLLTYLGIALLVGMLGSWLLSRRIKRQTRGMEPAEIAGLAEH
                     REAMLRGIAEGVIALDPQHRITLVNEVARQLLDLPEH"
     gene            872..1885
                     /locus_tag="PLK64_11545"
     CDS             872..1885
                     /locus_tag="PLK64_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HPV80426.1"
                     /translation="MRLHHIALGAILASALAVSGCGATKDQGAGDSSSAAAGSAAPAT
                     GLQYMVPNSPGGGYDTTARAAAQVMEKEGITGTVQVFNLPGAGGTVGLQRVVNEKGNG
                     KLAMQMGLGVVGAAYTQKTEASLNDTTPIAKLIEEAGAIVVPKDSPYQDINQLVEAWK
                     KDPKGLAVGGGSSPGGPDHLLPMQLAQAVGIDPKAVSFVSYDGGGELLPALLGSKIAF
                     GASGFGEFLDQVEAGDVRVLAVTSAERVDALKDVPTLKESGIDLEFTNWRGVVAPPGI
                     SEEDKAVWIDAFTKMHASAGWKAELEKRGWVDAFATGEEFGTFLKAQDTEVAEILKTL
                     GLA"
     gene            1882..2397
                     /locus_tag="PLK64_11550"
     CDS             1882..2397
                     /locus_tag="PLK64_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HPV80427.1"
                     /translation="MSVQDIAPATGVGDRAQYGVCAALALLGVVVIVDATRIGAATSS
                     NDPVGPKALPFIIGAALVLISVFYALDVARGGVGEAEGGEDVELGAPADWRTVGLLAG
                     AFVLNALLIERLGWVISGALLFAMSAYALGNRHHARGLAVGIVMSLTTFYAFAIGLGV
                     SLPAGILQGIL"
     gene            2397..3896
                     /locus_tag="PLK64_11555"
     CDS             2397..3896
                     /locus_tag="PLK64_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HPV80428.1"
                     /translation="MDNINGLLGGFADVITPMNLLIALLGVLIGTAVGVLPGIGPAMT
                     VALLLPLTYGLPVEQSLILFAGIFYGGMYGGSTTSILLNTPGESASVVTALEGNKMAK
                     SGRAAQALATAAIGSFVAGTIGTALIVLVMPQVVDFAITLGSPEFLAIILVAFVGTAA
                     ILGSSRVRGFAALLVGLTLGLVGTDFVTGQQRLTFGSPFLADNIDVVVIAVGIFAVGE
                     ALWVAAHLRRKAATVIPVGRPWLSREDWGRSWKPWLRGTALGFPFGAIPAGGAEIPTF
                     LSYLTERKLSKNPEEFGKGAIEGVAGPEAANNASAAGTMVPLLALGLPTNATAAIIIA
                     AFMSYGIEPGPLLLTKEPDLVWMLIASLFIGNFFLLVLNLPLAPTWAKLLRIPRPYLY
                     AGILFFASMGAYAVNGQPFDLYLLLALGFLGFAMRRFGIPVLPLIVGAILGPLFERHL
                     RRSLQTSGGDLSGLFGGPVAWVCYALIIAVLLWPLLARLRPSRQEGIAS"
     gene            3893..4291
                     /locus_tag="PLK64_11560"
     CDS             3893..4291
                     /locus_tag="PLK64_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPV80429.1"
                     /translation="MTVLVGYIPNPLGEATIRAGVEEARRRAEPLVVVNMSRDDVLVD
                     AHRAASDDLARVERDVAELGVEVEVVRIEQGSDPATAIVEAAAERGASVIVIGLRHRT
                     PVGKLIMGSVAQRILIDAACPVLAVKEGSA"
     gene            4288..4758
                     /gene="rraA"
                     /locus_tag="PLK64_11565"
     CDS             4288..4758
                     /gene="rraA"
                     /locus_tag="PLK64_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HPV80430.1"
                     /translation="MSVSTADLWDERGDELQSCSVQLRHFGGRTSFTGVISTVRCHRD
                     NALVKSALAEPAEGRVLVVDGGGSLESALMGDLIAASAVDNGWAGVVIHGVVRDVVAL
                     RSLDLGAVALGSNPRKSAKDGVGERDAPVSFGGVEFVPGQQIWVDEDGVVATHP"
     gene            complement(4869..5309)
                     /locus_tag="PLK64_11570"
     CDS             complement(4869..5309)
                     /locus_tag="PLK64_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPV80431.1"
                     /translation="MTRPASSETPDLGDLVMRVARTLRRRGAEAMSPWDIAPHHARAL
                     KVISRHEGIRPGELAGHLRVAPRSVTDVVDALEARGLLAREPDPGDRRATVLVLTASG
                     RQLMQEIGQARRADADTYFARLSATDRADLERILRTLDDRPDPA"
     gene            5530..>6352
                     /locus_tag="PLK64_11575"
     CDS             5530..>6352
                     /locus_tag="PLK64_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV80432.1"
                     /translation="MTIDPRDKAQLADAPVPLSRVLALFRPHRGRVAVVVAIIVVTSI
                     VSLAQPFLVREIVDVALPQQDVRLLLLAVGGMVAVAAITQLLGVLQTWLSTQVGQRVM
                     HELRTRVFTHLQRQSLGFFTRTRGGEVQSRLTNDVNGMQGVVTSTATSIAANLTTAVA
                     TAIAMVALSWRLALLSLLVIPPALWLTRKVALIRRAVTAQQQRHLADLNHQIDEGLSV
                     SGVRLAKTLGTSTRNAQRFATTSHELVDLELRSQLAGRWRMATMQIVFAVIPAVVY"
BASE COUNT          864 a         2302 c         2192 g          994 t
ORIGIN      
        1 gtgctcaggc aggtcgagca gctgccgggc cacctcgttg accagggtga tgcggtgctg
       61 ggggtccagg gcgatgaccc cctcggcgat gccgcgcagc atcgcctcac ggtgttcggc
      121 gaggccggcg atctcggccg gctccatgcc gcgggtctgg cgcttgatgc gtctcgagag
      181 cagccacgac ccgagcatcc cgacgagcag ggcgatgccc aggtaggtga gcaggtacga
      241 cgacgtgccc tggagccgtt cccacgtgga ggggacgcgc tcggcgacca tcgccgaacc
      301 gaggtgcgcg cccacggcat cctcggattc accgagcacc ggaacctgcg agacgaggtg
      361 cgcgacgccg tcgatctcca gggtgcccga ccagctcgcc ccgtcggcca cccgcgggtc
      421 gccgagcggc ataggggcgc cctcgaggga ggggtcggtg gagacgcgca cgtcgaggtc
      481 ggtctcggcg aagctcaccg aggaaaccga ggtctgcgtc acgaggctct gggcgagggt
      541 ggccaggccg gtgcgggtct cggtctcggc gaggctggcc cgcacgaggg ggctgctcgc
      601 gacctgctcg gcgagggcgg ccacccgccg cccctcgacg cgctcgaacg tcgccgccga
      661 ctgggcgagg gacacggcgg ccacggccac gaggacgacg gcgatgatcg cgagctgcag
      721 ggcgaacagc tgcccggcca gcgtcgatcg aggccgcctc cgcatgacca ctatgaactc
      781 aaccttctct gccgacacaa gcgcgacagc agcaactgcg cgcccccacg atcatggcgt
      841 ccgactctcc cagacgaaag cgatctcaac gatgagactc caccacatcg ccctcggcgc
      901 catcctcgcg tccgccctcg ccgtctccgg ctgcggggcg accaaggacc agggtgccgg
      961 cgactcgtcc tcggcagcgg ccggcagtgc agcccccgcc accggcctgc agtacatggt
     1021 tccgaactcc ccgggtggcg gttacgacac gaccgcccgc gccgcggccc aggtcatgga
     1081 gaaggagggc atcaccggca cggtgcaggt attcaacctc ccgggggccg gtggcaccgt
     1141 cggcctccag cgggtcgtca acgagaaggg caacggcaag ctggccatgc agatggggct
     1201 cggcgtcgtc ggcgcggcct acacgcagaa gaccgaggcc tcgctgaacg acaccacccc
     1261 gatcgccaag ctcatcgagg aggccggcgc gatcgtcgtg cccaaggact ccccgtacca
     1321 ggacatcaac cagctggtcg aggcgtggaa gaaggacccc aagggcctcg cggtcggtgg
     1381 cggctcctcc ccgggtgggc cggaccacct gctgccgatg cagctcgcgc aggcggtcgg
     1441 aatcgacccc aaggccgtga gcttcgtgtc ctacgacggc ggcggtgagc tgctgccggc
     1501 gctgctcggc agcaagatcg ccttcggcgc cagcgggttc ggcgagttcc tcgaccaggt
     1561 cgaggccggt gacgtgcggg tcctcgcggt gaccagcgcc gagcgcgtcg acgccctcaa
     1621 ggacgtgccc acgctcaagg agagcggcat cgacctcgag ttcacgaact ggcgcggggt
     1681 cgtggcacct cccggcatct ccgaggagga caaggcggtc tggatcgacg cgttcacgaa
     1741 gatgcacgcc tcggcgggct ggaaggccga gctcgagaag cgcggctggg tcgacgcctt
     1801 cgccaccggt gaggagttcg ggaccttcct caaggctcag gacaccgagg tcgccgagat
     1861 cctgaagacg ctcggcctgg catgagcgtc caggacatcg ctcctgcgac gggggtcggc
     1921 gaccgcgcgc agtacggcgt gtgcgcggcg ctggccctcc tcggggtggt cgtcatcgtc
     1981 gacgccaccc gcatcggggc ggccacgagc agcaacgacc cggtcgggcc gaaggccctg
     2041 ccgttcatca tcggcgccgc gctggtcctc atctcggtgt tctacgcact cgacgtcgcc
     2101 cgtggtggcg tcggtgaggc cgagggcggt gaggacgtcg agctcggcgc ccccgccgac
     2161 tggcgcaccg tcggccttct cgccggtgcc ttcgtgctca acgccctgct catcgagcgg
     2221 ctcggctggg tcatcagcgg agcgctgctg ttcgcgatgt cggcctacgc gctcggcaac
     2281 cgccaccacg cccgcggcct ggccgtcggg atcgtcatga gcctgacgac cttctacgcg
     2341 tttgcgatcg gtctcggcgt gagcctgccg gccggcatcc tgcagggaat cctgtgatgg
     2401 acaacatcaa cggcctgctc ggcggcttcg ccgacgtcat caccccgatg aacctcctca
     2461 tcgccctcct cggcgtgctc atcggcacgg ccgtgggcgt gctgcccggt atcggccccg
     2521 ccatgacggt cgcgctgctg ctgccactca cctacggcct gcccgtcgag cagtcgctca
     2581 tcctgttcgc cggcatcttc tacggcggca tgtacggcgg ctcgacgacg tcgatcctgc
     2641 tcaacacccc cggtgaatcg gcatcggtcg tcaccgccct cgagggcaac aagatggcca
     2701 agtcgggccg ggccgcgcag gccctggcca cggcggccat cggctcgttc gtcgccggca
     2761 ccatcggcac ggcgctcatc gtgctcgtca tgccgcaggt cgtcgacttc gccatcaccc
     2821 tcggctcgcc cgagttcctc gcgatcatcc tcgtggcctt cgtcggcacg gcggccatcc
     2881 tcggctcctc ccgggtgcgc ggcttcgcag ccctgctcgt cgggctcacc ctcggtctcg
     2941 tcggcaccga cttcgtcacc ggccagcagc ggttgacgtt cggcagtccg ttcctcgccg
     3001 acaacatcga cgtcgtcgtc atcgcggtcg gcatcttcgc cgtcggcgag gccctgtggg
     3061 tcgcggccca cctgcggcgc aaggcggcca cggtgatccc cgttggtcgt ccgtggctct
     3121 cgcgcgagga ctggggtcgc tcgtggaagc cctggcttcg cggcaccgcc ctcggcttcc
     3181 cgttcggggc gatccccgcc ggtggtgccg agatcccgac gttcctgtcc tacctcaccg
     3241 agcgcaagct ctcgaagaac cctgaggagt tcggcaaggg cgccatcgag ggtgtcgccg
     3301 gcccggaggc cgcgaacaac gcgtccgccg ccggcacgat ggtgccgctg ctcgccctcg
     3361 gactgccgac gaacgcgacg gccgccatca tcatcgcggc cttcatgagc tacggcatcg
     3421 agcccggccc cctcctgctc accaaggagc cggacctggt gtggatgctc atcgccagcc
     3481 tgttcatcgg caacttcttc ctgctcgtgc tgaacctgcc gctcgcgccg acctgggcca
     3541 agctgctgcg catcccgcgt ccgtacctct acgcggggat cctcttcttc gcgtcgatgg
     3601 gtgcgtatgc cgtcaacggc cagccgttcg acctctacct gctgctggcg ctgggtttcc
     3661 tcggcttcgc gatgcgtcgc ttcgggatcc cggtgctgcc gctgatcgtc ggggccatcc
     3721 tcgggccgtt gttcgagcgg cacctgcggc gctcgctgca gacctccggt ggtgacctgt
     3781 ccggcctgtt cggtgggccg gtcgcctggg tctgctacgc cctgatcatc gcggtgctgc
     3841 tctggccgct gctcgcacgc cttcgcccgt cgcgccagga aggaatcgcg tcatgaccgt
     3901 cctcgtcggc tacatcccga acccgttggg agaggccacc atccgcgccg gggtcgagga
     3961 ggcccgacgc cgggccgagc ccctcgtcgt cgtcaacatg tcgcgcgacg acgtgctcgt
     4021 cgacgcccac cgggcggcat ccgacgacct cgcccgggtg gagcgcgacg tcgccgagct
     4081 cggggtcgag gtcgaggtgg tgcgcatcga gcagggcagc gaccccgcga cggcgatcgt
     4141 cgaggccgcc gccgagcgcg gtgcctcggt catcgtcatc gggctgcggc accgcacccc
     4201 ggtgggcaag ctcatcatgg gctcggtggc gcagcgcatc ctgatcgatg ccgcgtgtcc
     4261 cgtcctggcc gtcaaggagg ggtcggcgtg agcgtctcga cggcggacct ctgggacgag
     4321 cgcggcgacg agctgcagtc ctgctcagtg cagttgcggc acttcggcgg gcgcacttcc
     4381 ttcaccgggg tgatcagcac ggtgcgctgc caccgggaca acgcgctcgt gaagtcggcc
     4441 ctggcagagc ccgcggaggg gcgggttctc gtcgtcgacg gcggcgggtc gctcgagtcg
     4501 gcgctcatgg gtgacctcat cgcggcatcc gcggtggaca acgggtgggc gggcgtcgtc
     4561 atccacggcg tcgtgcgtga cgtcgtggcg ctgcgctcgc tggatctcgg cgccgtcgcg
     4621 ctggggtcga acccgcgcaa gagcgccaag gacggggtcg gcgagcgtga cgcgcccgtg
     4681 tcgttcggag gtgtcgagtt cgtgcccggg cagcagatct gggtcgacga ggacggtgtg
     4741 gtcgccaccc acccctgacg gcccccacct ccgtccggaa cccatcccgg tcggaacttt
     4801 cccgcccgat gccagaaggt tcaccttcgc cccacttctt agctggggcg aaggtgaacc
     4861 ttctggtgtc aagcagggtc cggccggtcg tccagggtgc gcaggatgcg ctcgaggtcc
     4921 gcgcggtcgg tggcgctgag gcgggcgaag tacgtgtcgg cgtccgcgcg tcgggcctgc
     4981 ccgatctcct gcatcaactg ccgtccggat gccgtgagca ccaacaccgt cgcccgccgg
     5041 tcgccggggt cgggttctcg ggcgagcagg ccgcgcgcct cgagggcgtc gaccacgtcg
     5101 gtcaccgacc ggggcgcgac gcgcaggtgg ccggccaact cgccgggccg gatgccctcg
     5161 tggcggctga tgaccttcag ggcccgagcg tggtgcgggg cgatgtccca cggggacatg
     5221 gcctcggcgc cgcgtcggcg cagggtgcgc gccacccgca tcaccaggtc gccgaggtcg
     5281 ggggtctcgg acgaggcggg ccgggtcatg cggggaatgg tagcgcgagg tcgtgtgttt
     5341 gactcattgt gaggtaacct cacgactact tcgtccgaga ggaggacgcc cgtgacggcg
     5401 accctcactg aaacccgatc acctcacgcc ggcgcggccc acggccccgg cgtcacggca
     5461 tccaccccca tcttcggtcg cgcagaacgc ggcggccgag gcggcggcgg tggtgatcgt
     5521 ggcggcccga tgaccatcga cccccgcgac aaggcccagc tcgccgacgc ccccgtgccg
     5581 ttgtcccgag tgctcgcgct cttccgcccg caccgcggcc gggtggccgt cgtcgtcgcg
     5641 atcatcgtcg tgacgagcat cgtgtccctg gcccagccgt tcctggtgcg cgagatcgtc
     5701 gacgtcgcgc tgccccagca ggacgtccgc ctgctgttgc tcgccgtcgg cggcatggtc
     5761 gccgtcgcgg cgatcaccca gctgctcggc gtgctccaga cgtggctgtc gacccaggtc
     5821 ggtcagcgcg tcatgcacga gctgcgaacc cgcgtgttca cccacctcca gcgccagtcg
     5881 ctcggcttct tcacccgcac tcgcggcggg gaggtgcagt cgcgcctgac caacgacgtc
     5941 aacgggatgc agggcgtcgt cacctcgacc gccacgtcca tcgccgcgaa cctcacgacg
     6001 gccgtcgcca cggcgattgc gatggtcgcg ctctcgtggc gcctggcgct gctctcgctt
     6061 ctcgtcatcc cgccggccct ctggctgacg cgcaaggtcg ccctgatccg gcgagccgtg
     6121 accgcgcagc agcaacgcca cctcgccgac ctcaaccacc agatcgacga ggggctctcg
     6181 gtgagcggcg tgcgcctggc caagacgctc ggcacctcga cccgcaacgc gcagcgcttc
     6241 gcgacgacct cgcacgagct cgtcgacctc gagctgcgct cgcagctcgc cgggcgctgg
     6301 cggatggcca cgatgcagat cgtcttcgcc gtcatcccgg ctgtggtcta cc
//
LOCUS       DAOMZY010000208         6346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_11504_length_6346_cov_4.757590, whole genome shotgun sequence.
ACCESSION   DAOMZY010000208 DAOMZY010000000
VERSION     DAOMZY010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6346
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11504_length_6346_cov_4.757590"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="PLK64_11580"
     CDS             complement(<1..987)
                     /locus_tag="PLK64_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HPV80433.1"
                     /translation="MSELEAVNRYILERVQEGSFDGDSAVSLLRKLNGVDPSKDIAIV
                     GMSCRFPRADDYDEYWRNIINKVGVIGTFPEDRARDTGHAMKLKAGWLEHIGDFDAAF
                     FRISPKEALSMHPEQRIFLEAAWTCLEDAGYCGPSVEGSNVGVFVGVDHSYPMDYVAH
                     DAAHSLLDMTGSMSSVMASRIAFVLDLRGPNMVLDTACSSGLVAVHQACLSIRAGESD
                     LAIAGGLHLLSDTDASFDGVESTSGVLASFDRNSQGTVWGEGLGLVVLKAADRAIADN
                     DHIYAIIRGSAVNNDGRSNGITAPKASTQADVILRAWRDAGIDPEHLSYVEAH"
     gene            complement(996..5756)
                     /locus_tag="PLK64_11585"
     CDS             complement(996..5756)
                     /locus_tag="PLK64_11585"
                     /inference="COORDINATES: protein motif:HMM:NF026115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPV80434.1"
                     /translation="MALKEYLYTQVAERRLSVADAKQYLRELSTAAGPHDIAVIGMAG
                     RFPEAPDLTAYWDNLRAGRASIRDLPDSRKPNALDFIRRFHQDDLRREGRINPDGSLR
                     IDFARRGYLDQVDQFDAAFFGVGPREAAAMDPNQRVFLEVAYAALEDAGYGGRRVHGR
                     QVGTFVGIDHVEELKYKRLAAKDPLAVTGTWPGILASRLAYLFDLRGPSMVIDTACSS
                     GLVSVHQACRALRQQECELAIAGGLSSFYYEATTFSSEQRELESIESPDDTVRTFDRR
                     AKGTTWGEGIGVVVLKPLDAAIRDGDLVHAVIRGSAVNNDGASNGITAPNADAQEQLL
                     LSAWQDAQVDPRSITYVEAHGTGTVLGDPIEVRALTNAFRRHTPDRQFCGLGSVKPNI
                     GHLVGASGMASLLKVILMIEHGEFVPSVNIDDPNAFIDFVGSPAYVCDRTTAWTPDPG
                     GVRRAGVNSFGFSGTNAHVVLEQPPAQAAVEDGRAAYIFTVSAKTPDLLRRLIHATAT
                     RLATAVAPALSALSFTSTVGRGHYEHRVAIRAATITELVAALRALADEVPEGGPVPAG
                     VWRGECRVVSERKQVISSGELTETTRRQLSARADLAVTAALAAPSGDGLDAVCELYVA
                     GADVPWSRLFDGIPVRTAALPVYPFDPAPWWYRGEAMEPDSGAPLEEPAAAPRLGALL
                     GARALSSPDIDYFTAGLSSAHDWILTDHVILGQHIVPGTTYVELALEVAREYWPGPVT
                     LRDMAYAEPFVVEADSSCSIQIAATRTTLGLDLLFTSFADGRWHRHAECTVSPGAGPT
                     ESRDLDGLRAGLDGAGAPCFDLEPRESVIALGARWANEQIVAAADRTAIVRLDLAEPM
                     RADLVGAVFHVAMFDNAVNAISQKVGEGLYLPYFYREIQCLAPMPPSFHSIISVRPGP
                     EDGDLETITFDVDLVAPDGQVFARVRDYSIKRVDDRSAAAIRRSGGVPHYYRLAPVPT
                     HATPDAQAKPVGHIVLLSSGTPAALALADELAVLDPGLCVITPASGEAGRPLWTEVGP
                     AIGAALTHGPTHVVDAASWGRDDDPETTLLGHFTVLKAISARTTSGALRFCAVADHVY
                     GVGDDIARPTHRALAAMLAGLAHEDPDLDVLVLDVDPADDPRGAAVRVAEQLTVGSYR
                     YDRTWWDGSWYEQQLEDALPTAGEDQPVVHRDGVYVITGGTGDLGLEVARWLAAREQV
                     TIVLIGRTDWTDAARPESPHGLKIREYLAAIAASGSRAQMYAGDVADRARMAEILDEV
                     RRDLGVVRGVFHCAGLAGEGLAISKSEAAFAATLRPKIGGFGVIDDLTRSDDLDVLVL
                     FSSVLALFGDVGQTDYMAANAYLDAISTRRASRTRVMSINWPAWAEVGMAVRFGVTSQ
                     ANTVVPLTTAEAMRCLDDLFGSWLTPLLPGRLDLQELGRRLDRVRFRLNPALQRAVDE
                     RLRAATSTAAGSSGGGSALVMHEEYTPTERQLLVIWADVLQVPTLDLFDTFSDLGGQS
                     FLAIQLYKAINRAFPGLVEVADVYSFPTVEQMAAQIDKKSLAQAEPRTAHPEPGAPEA
                     GAGDSTVEISDLISQVKAGRTSIDDAVRIMIGKE"
     gene            complement(5758..>6346)
                     /locus_tag="PLK64_11590"
     CDS             complement(5758..>6346)
                     /locus_tag="PLK64_11590"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HPV80435.1"
                     /translation="VRMGLDESRNNLYSLANAEALQCFDAVLRHPRPHVVVGRPNRRV
                     LEQSSRTSMIFDDDERREQPLAEVRVTAPSAANGNGVYTTTETSVAAAWRAVLGNPVI
                     DVHANFTEAGGDSILAVRLLDALRADFGDLVDITSVFTYPTIHLMADYLDRHGQPVGA
                     ATTVSGNDPADDELDRILAGLASGDLSVQDAERLY"
BASE COUNT         1021 a         2110 c         2225 g          990 t
ORIGIN      
        1 gtgtgcctcg acgtaggaca ggtgctcggg atcgatgccg gcgtcccgcc acgcgcgcag
       61 gatcacgtcg gcctgggtcg aggccttggg ggcggtgatg ccgttgctgc ggccgtcgtt
      121 gttgacggcg ctaccgcgga tgatcgcgta gatgtggtcg ttgtcagcga tcgctcgatc
      181 ggcggccttg aggacgacca ggcccagtcc ctcgccccac accgttccct gcgagttccg
      241 atcgaaggat gcgaggacac cggaggtcga ctcgaccccg tcgaaggagg cgtcggtgtc
      301 gctgagtagg tgcagtccac cggcgatagc cagatcgctc tcgcccgccc ggatcgacag
      361 acaggcctgg tggaccgcga ccagaccgga ggaacaggcc gtgtccagca ccatgttcgg
      421 tccgcgcagg tccaggacga aggcgatccg gctggccatg accgaggaca tcgagccggt
      481 catgtccagc agggagtgag cggcgtcgtg ggcgacgtag tccatcggat aggagtggtc
      541 cacgccgacg aagaccccga cgttgctgcc ctcgacgctc ggcccgcagt agcccgcatc
      601 ctccaggcag gtccaggctg cctccaggaa gatgcgctgt tcggggtgca tgctcaaggc
      661 ttccttgggc gagatccgga agaaggccgc atcgaaatca ccgatgtgtt ccagccagcc
      721 ggccttgagc ttcatcgcat gaccggtgtc acgagcccgg tcctcgggga aggtgccgat
      781 gacgccgacc ttgttgatga tgttgcgcca gtactcgtcg tagtcatcgg cgcgagggaa
      841 gcgacacgac atccccacga tcgcgatgtc cttgctcggg tcgaccccgt tgagcttgcg
      901 caacaaggag actgccgagt ccccgtcgaa gctgccctcc tggacccgct ccaggatgta
      961 ccggttgacc gcctcaagct cgctcatcgg gacgctcact ccttcccaat catgattcgt
     1021 acggcgtcgt cgatggacgt ccgcccggct ttgacctggc tgatgaggtc gctgatctcg
     1081 accgtggagt cgccggcgcc ggcctcgggg gcgcccggct ccgggtgggc cgttcggggc
     1141 tcagcctgcg cgagggactt cttgtcgatc tgtgccgcca tctgctcgac cgtcgggaac
     1201 gaatagacgt cggcgacttc caccagcccc gggaaggccc gattgatcgc cttgtaaagc
     1261 tggatggcga ggaacgactg gccgccgagg tcgctgaacg tgtcgaagag atccagcgtc
     1321 ggaacctgca ggacgtcggc ccagatgacc aacagttggc gttcggtcgg ggtgtattcc
     1381 tcgtgcatca ccaacgccga gccaccaccg gagctgccgg cagcggtgct cgtggcggcc
     1441 cggagccgct cgtcgacggc tcgctgcagc gcgggattga gccggaaacg cacccggtcc
     1501 agccggcggc ccagctcctg caggtccaga cggcctggga gcagcggcgt cagccaggag
     1561 ccgaacaggt cgtccaggca ccgcatcgcc tccgccgtcg tgagcgggac gaccgtgttc
     1621 gcctggctgg tcaccccgaa tcgcaccgcc atgcccacct ccgcccaggc cggccagttg
     1681 atcgacatga ctcgcgtccg gctggcgcgg cgtgtggaga tcgcgtcgag gtaggcgttg
     1741 gccgccatgt agtccgtctg gcccacgtct ccgaagaggg cgaggacgga cgagaacagg
     1801 acgagcacat cgaggtcatc ggatcgcgtg aggtcatcga tgacgccgaa gccaccgatc
     1861 ttcggtcgca acgtggccgc gaaggccgcc tcgctcttgc tgatggccag accctcgccg
     1921 gccaggccag cacagtggaa gactccgcga acgacgccga gatcgcggcg aacctcgtcg
     1981 aggatctcgg ccatccgagc ccggtcggcg acgtcaccgg catacatctg cgcgcgtgac
     2041 cccgacgcgg cgatagcggc gaggtactcc cggatcttga gcccgtgcgg tgactcgggg
     2101 cgggccgcgt cggtccagtc ggtgcgcccg atgagcacga tcgtcacctg ctcgcgggct
     2161 gccaaccagc gggcgacctc gagcccgagg tcgccggtcc ctccggtgat cacgtagacg
     2221 ccgtcgcggt ggaccaccgg ctggtcctca cctgccgtcg ggagggcgtc ctcaagttgc
     2281 tgctcgtacc acgagccgtc ccaccaggtc cggtcgtatc ggtagctgcc caccgtcaac
     2341 tgctcagcca cccgcactgc ggccccgcgc ggatcgtcgg ccggatcgac gtcgaggacg
     2401 aggacgtcga ggtcagggtc ctcgtgcgcc aacccggcga gcatcgctgc gagcgcgcga
     2461 tgggtcggcc gggcgatgtc gtcgccgacg ccatagacat ggtcggcgac cgcgcagaac
     2521 cgaagcgccc ctgacgtggt gcgcgccgag atcgccttga gcaccgtgaa gtgcccgagc
     2581 aacgtcgtct cggggtcgtc gtcgcgcccc caggatgccg cgtcgacgac atgggtcggg
     2641 ccgtgtgtca acgctgcgcc gatggctggg cccacctcgg tccagagcgg ccggccggcc
     2701 tcccccgacg ccggcgtgat gacgcagagg ccggggtcga ggacggcgag ttcgtcggcc
     2761 agggcgagcg ccgccggagt gccgctgctg agcaacacga tgtgcccgac aggtttggcc
     2821 tgcgcatcgg gagtggcgtg ggtcggcacc ggggcgagcc ggtagtaatg ggggacgcct
     2881 ccggagcggc ggatcgccgc cgctgatcga tcatcgaccc gcttgatcga atagtccctg
     2941 acccgggcga agacctggcc atcgggagca acgaggtcca cgtcgaaggt gatcgtctcc
     3001 aagtcaccgt cctccggtcc gggtcggacg gagatgatgc tgtggaacga cggcggcatc
     3061 ggggccaggc actggatctc acgatagaag taggggaggt agaggccctc gccgaccttc
     3121 tggctgatgg cgttgaccgc gttgtcgaac atcgccacgt ggaacacggc accgacgaga
     3181 tccgcccgca tcggctcggc gaggtcgagg cgcacgatcg cggtgcgatc cgcagccgcg
     3241 acgatctgct cgttggccca ccgcgctccc aacgcgatga cggactcgcg cggttcgagg
     3301 tcgaagcacg gcgcccccgc gccgtcgagt cctgctcgga ggccgtcaag gtcgcggctc
     3361 tcggtgggtc cggccccggg gctgaccgtg cattcggcat gccgatgcca cctgccgtcg
     3421 gcgaagctgg tgaagagcag gtccagcccg agcgtcgtgc gagtcgctgc gatctggatc
     3481 gagcagctcg aatctgcttc tacgacaaag ggttcggcgt aggccatgtc gcgaagcgtc
     3541 acgggacccg gccaatactc ccgggcgacc tccagggcca gctcgacgta ggtggtcccg
     3601 ggaacgatgt gttggccgag gatgacatgg tcggtgagga tccagtcgtg cgcgctggac
     3661 agcccagcgg tgaagtagtc gatgtccggg gagctgagcg cgcgggcacc cagcagcgcc
     3721 cccagtcgcg gcgccgcggc cggctcctcg aggggtgcgc ccgagtcggg ttccatcgcc
     3781 tcgccgcggt accaccacgg agccgggtcg aagggataca ccggcagggc cgccgttcga
     3841 acgggtatgc cgtcaaacag gcgactccac gggacgtccg ctccggcgac atagagctcg
     3901 cagacggcgt ccaacccgtc acccgacggc gcagccaacg ccgccgtgac cgccaggtcc
     3961 gcgcgagcgg acagttgccg gcgggtggtc tcggtgagct cgcccgacga gatcacctgc
     4021 ttgcgctcgg agacgacccg gcactctccc cgccacacgc ctgcgggcac aggcccaccc
     4081 tcgggcacct cgtcggcgag ggcgcgaagg gcggcgacca gttcggtaat ggtggcggcg
     4141 cggatggcga cgcgatgctc gtagtgaccc cgcccaacgg tggaggtgaa gctcagcgcc
     4201 gacaacgccg gcgccacggc cgtggccaac cgggtggccg tcgcgtggat gagacggcgg
     4261 agcaggtccg gcgtcttcgc cgagacggtg aagatgtatg ccgcgcgccc gtcctcgacg
     4321 gccgcctgcg ccgggggttg ttcgaggacg acgtgggcgt tggtgccact gaacccgaac
     4381 gagttgaccc ccgcccgtcg gacgccgccg gggtctgggg tccacgctgt ggtccggtcg
     4441 cagacgtagg ccggcgagcc gacgaagtcg atgaacgcat tggggtcatc gatattgacg
     4501 ctcgggacaa actcgccatg ctcgatcatg aggatgacct tgagcagtga ggccatgccc
     4561 gatgctccaa cgaggtggcc gatgttgggc ttcaccgagc ccagcccgca gaactgccga
     4621 tccggcgtat gccgccggaa ggcgttggtg agcgcccgca cctcgatggg gtcgccgagc
     4681 accgtccccg ttccgtgggc ctcgacataa gtgatcgatc gcggatcgac ctgggcgtcc
     4741 tgccaggcgc tcaacagaag ttgctcctgg gcatccgcgt tgggagcagt gatcccgttg
     4801 gacgccccgt cgttgttgac cgcactcccc cggatcacgg cgtggaccag gtcgccgtcg
     4861 cggatcgcgg cgtcgagcgg tttcaacacg acgaccccga tgccctcacc ccaggtcgtg
     4921 cccttggccc ggcggtcgaa ggtgcgaacg gtgtcgtcgg gggactcgat cgattccaac
     4981 tcgcgttgct ccgacgagaa cgtcgtcgcc tcgtaataga agctgctcag ccccccggcg
     5041 atcgccaact cacactcctg ctggcgcagt gctcggcagg cctgatgcac cgacacgagg
     5101 ccggaggaac acgccgtgtc gatgaccatg ctcggacctc ggaggtcgaa caggtaggcc
     5161 aatcggctgg cgagaatgcc aggccaggtt ccggtgacgg cgagcgggtc cttcgccgca
     5221 agacgcttgt acttcagctc ttcgacgtgg tcgatgccga cgaacgtccc gacctggcgg
     5281 ccgtggacgc gtcggccgcc gtagcctgcg tcttccagcg cggcatacgc cacctccaag
     5341 aagacccgct ggttggggtc catcgcggcg gcctcgcgtg gccccacgcc gaagaaggcg
     5401 gcgtcgaact ggtcgacctg gtcgaggtat ccgcgccgcg cgaagtcgat gcgcagcgac
     5461 ccgtccgggt tgatccggcc ctcgcgccgc aggtcgtcct ggtggaagcg acggatgaag
     5521 tccagggcgt tgggcttgcg cgagtcgggc aggtcacgga tcgacgcgcg ccccgcacgg
     5581 aggttgtccc agtaggccgt caggtcgggt gcctcgggga agcggcccgc catcccgatg
     5641 acggcgatgt cgtgcgggcc ggcagcggtg gacaactctc ggaggtactg cttggcgtcg
     5701 gcgacgctga gccggcgctc cgcgacctgg gtgtagaggt actccttgag ggccatgtca
     5761 gtagagcctc tccgcgtcct gcaccgacag gtcaccgctg gccaacccgg ccaggatccg
     5821 gtcgagctcg tcgtcagccg ggtcgtttcc ggagaccgtc gtggccgccc ccaccggctg
     5881 gccgtgccgg tcgaggtagt cggccatcag gtggatcgtg ggataggtga acaccgaggt
     5941 gatgtcgacg agatcgccga agtcggcccg cagtgcgtcg agaagccgca cagcaaggat
     6001 cgaatcaccg ccagcttcag tgaaattggc gtgcacgtcg atcacgggat tcccgaggac
     6061 cgcccgccag gcggcggcga cgctggtctc ggtggtcgtg tagacgccgt tcccattggc
     6121 ggcggagggg gcggtgaccc tcacctcagc caacggctgc tcacgtcgct cgtcgtcgtc
     6181 gaagatcatc gaggtccgag acgattgctc cagcacccga cggttgggcc ggccgacgac
     6241 cacatggggc cgcggatggc gcaggacggc gtcgaaacac tgcagcgctt cggcgttggc
     6301 cagcgagtag aggttgttgc gcgactcgtc caggcccatc cggacc
//
LOCUS       DAOMZY010000209         6317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_11592_length_6317_cov_5.839189, whole genome shotgun sequence.
ACCESSION   DAOMZY010000209 DAOMZY010000000
VERSION     DAOMZY010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11592_length_6317_cov_5.839189"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2284)
                     /locus_tag="PLK64_11595"
     CDS             complement(<1..2284)
                     /locus_tag="PLK64_11595"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HPV80436.1"
                     /translation="MRLHRLQARAFGPFAELIEVDFDELADAGIFLIHGATGSGKTSL
                     LDAVCFALYGRVPGLRPAGETLRSDHAGADDVPEVTLELTVGNRRLRVTRSPDHHRPK
                     KRGNGLTPVRGQVRLDELSRDGVWTTLATRADDVGDSLGQALGMGLDQFAKVVLLPQG
                     DFAAFLRATPEERRVLLERLFDVSRYTGVEAWLTDRRRLTAATAAEARATLSADLLRV
                     DDVLARVPAEVIDGLPTWAEATEAGRLAEVAAEASTRVGAHALAALTAAATAEQAASA
                     AESAHAAAALTARAAARGEAARRVLAEFAAGSDARAAAARRLELGERAGRVAGDLRAL
                     AAAGAERDSAHTRYAASRTALARWSVDVGDSPQEALSRARDLDATLDVLAQRGAAEAT
                     RTQRQASVSVQRGVAQRALATIDAAEAALVEQVAQLQVRADALLTIAAGSGPAEASAS
                     QAGRLVTLRDDLDRTAITIASLESAGLAATRVALAQAGRLLDLRQRRLDGMAAELAAG
                     LHPGDECPVCGGTEHPAPATAVDAVTAADVDAAEEAWRPLDDRVRRIETDLAAARAHH
                     DLLVEQLGGDERDLATLRDAADVIGRAVAVAASAVAELPQVQAELAQRRQALETLAET
                     RRAALGQLAAAQALEGELAEAAEHDAAVVRELVARHTEGCPCADPQVPTADVPARHRR
                     VVAALADTAAARSASDEAQRRHAEAHEATSSVCRAEGFADVVQACAAAVPAATLVEIR
                     EGIAAKVAAAAAARATVADPE"
     gene            complement(2281..3456)
                     /locus_tag="PLK64_11600"
     CDS             complement(2281..3456)
                     /locus_tag="PLK64_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HPV80437.1"
                     /translation="MRIVHTSDWHLGRSFHQVGLLGAQVQFVEFLVDLVRTEGVEAVL
                     VSGDVYDRALPSPDTVSVLADALTRLVDAGAQVVLTSGNHDSAIRLGFASDLLARAGV
                     HIGASLSSVGTPVVLGDVAVYPLPYLEPAVVGPALGVGERSHAAVLRAAMDRVRADLT
                     SHDLPSVVMAHAFVAGGTTTESEREIAVGGVSVVPADVFNGVTYAALGHLHRPQWVRD
                     DVAYSGSPLAMSFGEADQIKSVSLLDLTATGVSVEAIPTPVPRRLAVLRGDLADLLTD
                     PTLEWARGAWCRAVLTDPVRPLAAYEQLTRRFPDLLHLEFAPRGGELHATSRYAARVP
                     SARAELDVCCDFLAHVRAGAAASDDERATLAEALDASRAARAEADDEGVADQSRGVA"
     gene            3565..5046
                     /locus_tag="PLK64_11605"
     CDS             3565..5046
                     /locus_tag="PLK64_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HPV80438.1"
                     /translation="MGQDIESITYTREQRQRFRQKVHLCLDVFERMLAYSQFDFERPM
                     TGMEIELNLTGSDFRPKMDNALVLERIADPEYQTELARYNIEFNVKPRELGGDSMLSL
                     EGDLRASLNRAHETSAEVGSKIVMVGILPTVTLDDFAGDWMSANARYHALNDAMLGAR
                     GEDVHLDIEGPTGEHLERYFDSLAPEAACTSMQLHLQVAPLSFADHWNAAQALAGAQL
                     ALAANSPFFLGKRLWAESRIPVFSQAVDTRSPELKNQGVRPRVWFGERWITSIFDLFE
                     ENVRYFPALLPETTDEDPSAVLDAGGVPALAEMRLHNGTVYRWNRPIYDIGGGRPHVR
                     VENRVLPAGPTIVDTLANAAFYYGALRMLAQEDRPVWSRMSFAAAEDNFLAGARDGLE
                     AKLYWPGRGDLPATELILRHLLPLANEGLRDWGVSTEARDRYLSVIEGRCLTGVNGAT
                     WQTATVAALEERGIPREEALRQMVATYSEHMHANTPVHTWPMP"
     gene            5043..5915
                     /locus_tag="PLK64_11610"
     CDS             5043..5915
                     /locus_tag="PLK64_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HPV80439.1"
                     /translation="MTRAFRLLNVFALADDPFSGNPLAVIEDASGLDGATMLAIARQF
                     NLSETTFVTPGGDTGGAGPTDATVRIFTPGYEMPFAGHPTLGTACVVGERLGKNHVVL
                     RVPAGDIPVDGLNVPHSGDQTWTLTAKPATITEGAVARSDLASAVGLNADDLVDEGWW
                     VDAGVDQFLTEVTSVQAVRRAAADPVAARRFILSPRGESMALVWAWTSPDTIEARFFF
                     SQDAAMLEDPATGSAAANLGSMLADRGFRGAVTVDQGAAVARPSRLHIEVTEAGRVRV
                     GGLVREIGRGTLAL"
     gene            5915..>6317
                     /locus_tag="PLK64_11615"
     CDS             5915..>6317
                     /locus_tag="PLK64_11615"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HPV80440.1"
                     /translation="MPTYVAFLRAVNIGRRQTPMARVRATLEASGFTDVQTYIQTGNV
                     LLTTTARSPEKVAQRVREVLSGDFGFDIPVVVRRPRDLVDLVAAVDALPDPFPQGRTY
                     AAFLDQEPSDAATAVLNDWSARDLQAWEYQPL"
BASE COUNT          946 a         2270 c         2247 g          854 t
ORIGIN      
        1 cctcgggatc ggcgacggtg gcccgtgccg cggccgcggc ggcaaccttc gccgcgatgc
       61 cctcccgaat ttcaaccagc gtggccgcgg gaacggccgc cgcgcaggcc tgcacgacat
      121 cggcgaagcc ctcggcgcgg catacggaag aggttgcctc gtgcgcctcg gcatgccgcc
      181 gctgtgcctc gtcggacgcc gagcgcgcgg ccgcggtgtc cgccaacgca gcgacgacgc
      241 ggcggtggcg cgctgggacg tcggccgtgg gcacctgcgg gtcggcgcac ggacacccct
      301 cggtatgccg tgccaccagc tcgcggacca cggccgcatc gtgctcggcc gcctcggcca
      361 gctcgccttc gagagcctgg gcggcggcga gctgcccgag ggctgcccgt cgggtctcgg
      421 cgagcgtctc gagggcctgg cggcgttgcg cgagctcggc ttgcacctgg gggagctcgg
      481 ccactgcgga ggcagcgacg gcgacggctc ggccgatgac gtctgcggcg tcgcgaaggg
      541 tcgccagatc gcgctcgtcg ccgccaagtt gttccacgag gaggtcatgg tgagctcggg
      601 cagcggccag atcggtctcg atgcggcgga cccgatcgtc cagggggcgc caggcctcct
      661 cggccgcgtc aacatcggca gcagtgacag catcgaccgc ggtcgccggg gctgggtgct
      721 cggtgcctcc gcagaccggg cattcgtcac ccgggtgcag gccggccgcg agctcagcgg
      781 ccatcccgtc caggcgccgt tggcgaaggt cgagcaacct cccggcctga gcgagggcga
      841 cccgggtggc ggccaagccg gccgactcaa ggctcgcgat ggtgatcgcc gtgcggtcga
      901 gatcgtcgcg gagagttacc agtcgcccgg cctgggacgc tgacgcctcg gccggcccgg
      961 atccggcggc gatggtcagc aacgcgtcgg cccggacctg caactgagcg acctgctcca
     1021 ccaacgccgc ctcggctgcg tcaatggtcg ccagcgcgcg ctgcgcgacg ccacgctgga
     1081 cggacacgct cgcctgccgt tgcgtccgcg tcgcctccgc tgcgccgcgc tgggccaaca
     1141 cgtcgagcgt ggcgtcgaga tcgcgggctc gggacagcgc ctcctggggc gagtcaccca
     1201 cgtcaaccga ccaccgggcg agggcggtac ggctcgcggc ataccgggtg tgggccgagt
     1261 ctcgctcggc cccggcggcg gcgagcgcgc gcaggtcacc agccacccgc ccggcccgct
     1321 cgcccagctc caggcgccgg gccgccgccg ctcgagcatc ggagcccgcc gcgaactctg
     1381 cgagcacccg gcgtgcggcc tcgccgcgag cagcggcccg cgccgtgaga gccgctgctg
     1441 catgggcgct ctcggccgca ctggcagcct gctcggccgt ggccgcggcc gtgagagccg
     1501 ccagggcatg ggcgccaacc cgggtcgacg cctcggcagc gacctcggcc agccggccag
     1561 cttcggtcgc ctcggcccac gtgggcagcc cgtcgatgac ctcggccgga acccgggcca
     1621 gcacgtcgtc gacccgcagc agatcggcgg agagtgtggc gcgcgcctcg gccgctgtgg
     1681 cggcggtgag gcggcggcgg tcggtcagcc aggcttcgac accggtgtag cggctgacgt
     1741 cgaagaggcg ctccagcagc acccggcgtt cttccggggt cgcccgcagg aaggccgcaa
     1801 agtcaccctg ggggaggagg accaccttgg cgaactggtc caggcccatg cccaacgctt
     1861 ggccgaggct gtcacccacg tcatcggctc gggtggccag cgtcgtccac accccgtcgc
     1921 gggacagctc gtcgaggcgc acctgaccgc ggaccggggt cagaccgttg ccacgcttct
     1981 tgggacggtg atggtccgga gaccgggtca cccggagcct gcggttgccg acggtgagct
     2041 cgagcgtcac ctcggggacg tcatcggctc ccgcgtgatc gctgcgcaac gtctcaccgg
     2101 ccgggcgcag acccggcacc cggccataga gcgcgaaaca gacggcatcc aggaggctgg
     2161 tcttgccgct cccggtggcc ccgtggatga ggaagatgcc ggcgtcagcg agctcatcga
     2221 aatcgacctc gatcagctcg gcgaacggac cgaacgcgcg cgcctgcagc cggtgcagcc
     2281 tcatgccaca ccccgggact ggtcggccac gccttcgtcg tcggcctcgg cccgggctgc
     2341 cctgctggcg tcgagggcct cggccagggt ggcgcgctcg tcatcggagg cggccgctcc
     2401 cgcgcgcaca tgggcgagga agtcgcagca gacgtcgagc tcggcgcggg cggaggggac
     2461 gcgagcggca tacctgctcg tggcgtgaag ctccccaccg cgcggggcga actcgaggtg
     2521 cagcaggtcg gggaaacggc gggtcagttg ctcgtatgcc gcgagcggcc ggaccgggtc
     2581 agtgaggacg gcccggcacc aggcaccgcg agcccactcc agggtggggt cggtcaacag
     2641 gtcggcgagg tcgccgcgca ggacggccaa ccgccggggc accggggtcg ggatcgcttc
     2701 gacggacacg ccggtggccg tgaggtcgag cagggagacc gacttgatct gatcggcttc
     2761 accgaaggac atcgccaacg gcgagccgct gtaggcgacg tcgtcgcgga cccactgggg
     2821 gcggtgcagg tgacccagag cggcatacgt cacgccgttg aagacgtcgg ccgggacgac
     2881 actcacgccg ccgacggcga tctcgcgctc agactcggtc gtcgtgccgc cggcgacaaa
     2941 ggcgtgggcc atgacgaccg agggcagatc gtggctcgtc aggtcagccc ggactcgatc
     3001 catcgcggcc cgcagcacgg cggcgtgcga ccgctcgccg acccctagcg ctggaccgac
     3061 caccgcaggc tcaagatagg gaagcggata gacggccacg tcgccgagga cgaccggggt
     3121 gccgacgctg ctcagcgacg caccgatgtg caccccggct cgggccagca ggtcggaggc
     3181 gaagccgagc cggatcgccg agtcgtggtt gccgctggtg aggacgacct gggcaccggc
     3241 gtcgactaac cgggtcagcg cgtcggcgag cacgctcacg gtgtcagggg aaggcagcgc
     3301 ccggtcgtag acatcgcccg acacgaggac ggcctcgacc ccctccgtgc ggaccagatc
     3361 gacgaggaat tcgacgaact gcacctgtgc accaagcagc cccacctggt ggaacgaacg
     3421 ccccaggtgc cagtcggagg tgtgaacgat gcgcacccgg ccaggctatg accggccacc
     3481 gacagggccc gggaggtggg tcggcgtgcc tcccgtggac ccaccatcgc gcgcgaaccg
     3541 ggcagagcgg catactctcc ggccatgggc caggacatcg agtcgatcac atacacgcgc
     3601 gagcagcgcc aacggttccg gcagaaggtg cacctgtgcc tggacgtgtt cgagcggatg
     3661 ctggcctaca gccagttcga cttcgagcgg ccgatgaccg ggatggagat cgaactcaac
     3721 ctgacgggaa gtgacttccg ccccaagatg gacaacgctc tcgtcctcga gcggatcgcc
     3781 gacccggagt atcagaccga gttggcccgc tacaacatcg agttcaacgt caagccgcgc
     3841 gagttgggcg gcgactcgat gctgtcgctc gagggcgacc tgcgcgcatc gctcaatcgg
     3901 gcccatgaga ccagcgccga ggtcgggagc aagatcgtca tggtcggcat cctccccacc
     3961 gtgaccctcg acgacttcgc gggcgactgg atgagcgcca acgcccgcta ccacgccctc
     4021 aacgacgcca tgctcggcgc ccgtggcgag gatgttcacc tggacatcga ggggcctacc
     4081 ggcgagcacc tggagcgcta tttcgactcc ctggcgcccg aggcggcctg cacgtcgatg
     4141 cagttgcacc tgcaggtggc gcccctgtcg ttcgccgacc actggaacgc cgcccaggcc
     4201 ctcgcgggcg cccagttggc cctggccgcc aactcgccgt tcttcttggg caaacggctg
     4261 tgggccgaat cccggatccc ggtgttcagc caggccgtcg acacccggtc gccggagttg
     4321 aagaaccagg gggtgcggcc gcgcgtctgg ttcggggagc ggtggatcac gtcgatcttc
     4381 gacctgttcg aggagaacgt gcggtacttc ccggcgctcc tgcccgagac caccgacgaa
     4441 gacccgtcgg ccgtgctcga tgccggaggg gtgccagccc tggctgagat gcgcctgcac
     4501 aacggcaccg tctaccggtg gaaccgcccg atctacgaca tcggcggcgg ccggccacac
     4561 gtgcgcgtcg agaaccgggt gctgcccgcc gggccgacca tcgtcgacac cctcgccaac
     4621 gccgcgttct actacggcgc cctgcggatg ctcgcccagg aggaccgccc agtgtggtcg
     4681 cgcatgagct tcgccgcggc cgaggacaac ttcctcgccg gggccaggga cggcctggag
     4741 gccaagctct actggccggg ccgaggtgac ctgccggcca ccgagctcat cctgcggcac
     4801 ctgttgccgc tggccaacga gggcctgcgg gactggggtg tctcgacgga ggcgcgggac
     4861 cgctacctgt cggtcatcga gggccgttgc ctcacggggg tcaacggcgc gacctggcag
     4921 acggcgaccg tcgcggcgct ggaggagcgc ggcatacctc gggaggaagc ccttcgccag
     4981 atggtggcga cctactctga gcacatgcat gccaacaccc ccgtccacac ctggccgatg
     5041 ccgtgacccg cgcgttccgc ctgctcaacg tcttcgcgct cgctgacgac cccttctcgg
     5101 gcaacccgct ggccgtcatc gaggacgcgt ccgggctcga cggcgccacg atgctcgcga
     5161 tcgccaggca gttcaacctg tccgagacga ccttcgtcac gcccggcggc gacacgggcg
     5221 gcgccggacc aaccgacgcg acagtgcgga tcttcacgcc cggctacgag atgccgttcg
     5281 ccggtcaccc gaccctcggc acggcctgcg tcgtcgggga gcgcctgggc aagaaccacg
     5341 tcgtcctgcg tgtccctgca ggggacatcc ccgttgacgg gctgaacgtt ccacacagcg
     5401 gcgaccagac ctggaccctc accgccaaac cggccacgat caccgaaggg gcggtcgctc
     5461 ggagcgacct cgcgtccgcc gtcgggctca acgccgacga cctcgtcgac gagggctggt
     5521 gggtcgacgc cggcgtggat cagttcctca ccgaggtgac gtcggtccag gccgtccgac
     5581 gagccgcggc cgacccggtg gcggcccggc gcttcatcct ctctccgcgc ggcgagagca
     5641 tggccctggt gtgggcatgg accagtcccg acacgatcga ggcgcggttc ttcttcagcc
     5701 aggacgccgc gatgctcgaa gacccggcga ccgggtccgc ggcggccaac ctgggcagca
     5761 tgttggccga tcggggcttc cgcggggcgg tgacggtgga ccaaggtgct gcagtggcgc
     5821 gcccgtcccg gctgcacatc gaggtcaccg aggccggccg agtccgggtc ggcggattgg
     5881 tccgcgagat cggccgcggc accctcgcgc tctgatgccg acctacgtcg cgttcctgcg
     5941 cgcggtgaac atcggtcggc gccagacccc gatggcccgc gtgcgggcca ccttggaagc
     6001 gagcgggttc accgacgtcc agacctacat ccagaccggc aacgtcctgc tcaccacgac
     6061 cgcgcgctcg ccggagaagg tcgcccagcg ggtccgcgag gtcctctccg gcgacttcgg
     6121 cttcgacatc cccgtcgtgg tccgtcggcc gcgtgatctg gtcgatctcg tcgccgccgt
     6181 cgacgccctg cctgatccgt tcccgcaggg gcgcacgtat gccgcgttcc tcgaccagga
     6241 gccgagcgac gcagcaaccg cagtgctcaa cgactggtct gcgcgcgatc ttcaggcgtg
     6301 ggaatatcaa ccgctcg
//
LOCUS       DAOMZY010000210         6299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_11639_length_6299_cov_4.464766, whole genome shotgun sequence.
ACCESSION   DAOMZY010000210 DAOMZY010000000
VERSION     DAOMZY010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6299
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11639_length_6299_cov_4.464766"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="PLK64_11620"
     CDS             <1..1007
                     /locus_tag="PLK64_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPV80441.1"
                     /translation="MLLLIVFGGLVGGIIWNLFTWLLGLPSSSSHALLGGLVGGTIGA
                     LGFAGVKWAGDGSKLDGVIGKVLLPSLLSPVIAMIVAATATRLIFMITARVEDKFRER
                     RFRWGQVGAASLVSLAHGTNDAQKTMGVITLALVAAGSVSSTAVYVPLWVKLSAALAI
                     ALGTYLGGWRIIRTMGKGLVDISSPQGLAADASSATVILASSQLGFALSTTHVATGSI
                     MGVGIGKKGASVRWSVAGRMVAAWAITFPAAAVMGAVMYWIAAGIGGIVGPLVVFLLL
                     VGLSFYMWKRSRVNAVDHKNVTDEWHDGSKPEVAVAAHDVIDPHHELDAPQPAGPAAH
                     "
     gene            1026..1331
                     /locus_tag="PLK64_11625"
     CDS             1026..1331
                     /locus_tag="PLK64_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80442.1"
                     /translation="MNMIPLALDGAWKVLVAGLLLGAGLPVLYAFAVRGLAMARGAGA
                     IIGEQTAKPFGKVIAWVLLLVILLAVSYGISFIVATGIGKVVTFDHILPWFVDKPKK"
     gene            1645..2898
                     /locus_tag="PLK64_11630"
     CDS             1645..2898
                     /locus_tag="PLK64_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPV80443.1"
                     /translation="MTTATVILVMVVLTALAFDFTNGFHDTGNAMAASIATGALSPKM
                     AVTLAATLDLVGALISVKVALTITNAVIDGIQMKTGVPNPQFIGSDNGKQGTILLMVV
                     LAGLVGGIVWNLLTWLLGLPSSSSHALLGGLVGATIAGFGFSQVNWLGHSTEKLDGVV
                     GKVLIPSLISPVIAFIVAAVGTRIVFSLTSKMGDKFRESRFRYGQVGAASMVALAHGT
                     NDAQKTMGVITLALIAAGQVDVSQEISIPFWVKLSAAVAIALGTYLGGWRIMRTLGKG
                     IVDINAPQGFAGDTSTASVILASSGLGFALSTTHVASGSIMGAGVGRKGAEVRWSVAG
                     RMASAWAITFPAAGLVGAVMFFISNLMPQLLGATVVMAILIAVSLYIWMRSRKDPVDK
                     HSVGKDWEAEPDAAHVAEAVPHAHH"
     gene            2917..3207
                     /locus_tag="PLK64_11635"
     CDS             2917..3207
                     /locus_tag="PLK64_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80444.1"
                     /translation="MNNLIIALDGAWKVLLAGLLLGAGIPTLFAMAVKGLAMAEGGEA
                     AGGASPRAAGKLMAAVFFFIILTLVVYGLAWLIFTSLGFKVGFSGPFPTFSK"
     gene            3221..3544
                     /locus_tag="PLK64_11640"
     CDS             3221..3544
                     /locus_tag="PLK64_11640"
                     /inference="COORDINATES: protein motif:HMM:NF023257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3349 domain-containing protein"
                     /protein_id="HPV80445.1"
                     /translation="MSPHTESAGRRLVNWLRAGRPEGIAQGDYIALMGVLHRKLTTAE
                     VESIAVSLVERDYDDELITEDEIRAMIDEKVHEEATEDDIARIAHLLEEAGVHVDPHV
                     PGATH"
     gene            3670..3975
                     /locus_tag="PLK64_11645"
     CDS             3670..3975
                     /locus_tag="PLK64_11645"
                     /inference="COORDINATES: protein motif:HMM:NF023257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3349 domain-containing protein"
                     /protein_id="HPV80446.1"
                     /translation="MGRYRGILRFALPGSGARPTTAVPLIALLGRRLSDQEVTDLEET
                     LAERARARGCDSLAEIDLVIAITLLTDELPTTRDIDRVRDALGRRGLTVQRRVPQPT"
     gene            complement(3995..4282)
                     /locus_tag="PLK64_11650"
     CDS             complement(3995..4282)
                     /locus_tag="PLK64_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005274838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HPV80447.1"
                     /translation="MADTAERVEAAHAPLSDRDREVLAFERQWWKYAGAKEQAIKELF
                     DMSATRYYQVLNALIDNQSALETDPMLIKRLRRLRASRQRQRSARRLGLNP"
     gene            complement(4415..5074)
                     /locus_tag="PLK64_11655"
     CDS             complement(4415..5074)
                     /locus_tag="PLK64_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPV80448.1"
                     /translation="MTARRGGLAWPTVVFDLDGTLVDTINLIVASYQHAFRTVLGREE
                     DEARIRAWIGQPLLRCFRESSPAHADALFAAYTTWNEANTERLVRGYAGVAELLLELT
                     SQGARVAVATSKRHPPAAKALALTGLTDLVPVLVTMEDTTVHKPDPTPLLLAVERAGG
                     EPGRAAYVGDAVVDVRAAQAAGMAGIGVLWGAGTEADLRAAEPLGVASNVAALRALLL
                     P"
     gene            complement(5071..5544)
                     /locus_tag="PLK64_11660"
     CDS             complement(5071..5544)
                     /locus_tag="PLK64_11660"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPV80449.1"
                     /translation="MTRPELPAQSNEPVSPELFAYLATDPVADLLGIVFEDIRPGYAR
                     ARMPVTHRLLNAPGTAHGGAMMALIDVVHAAVSNSHGTLAVAQDVHTEFLSAGVEGDA
                     LRCEGVEISKTARTAVFRIDVFADDRPGHPDGKLLATALARVFRIGTPLLAGPAS"
     gene            complement(5554..>6299)
                     /locus_tag="PLK64_11665"
     CDS             complement(5554..>6299)
                     /locus_tag="PLK64_11665"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HPV80450.1"
                     /translation="PRPAMLLSTRRALAEALGTGALVATVVGSGIMATRLTQDVAVQL
                     LVNALATIAALAVLIWALGPISGAHFNPAVTLVALVRRELGAAEACRYVIAQIVGGLA
                     GTAVGNLMFDLPAWQASMKVRSSPSLWLGEVVATAGLLSLVGGLTRSGQGRLGPALVP
                     AWIGAAYFFTSSTSFANPAVTIARSATDTFSGIAPGSVAGFIGAQLVGAALGAGLTEF
                     FHPRAGVPEPLDLPAAVHPAPERASAYGG"
BASE COUNT          982 a         2221 c         2057 g         1039 t
ORIGIN      
        1 ccatgctgtt gctcatcgtc ttcggtgggt tggtcggcgg gatcatctgg aacctgttca
       61 cctggctgct gggcctgccc tcgtcctcgt cgcacgccct cctcggcggc ctggtcggtg
      121 gcaccatcgg cgccctcggt ttcgccgggg tcaagtgggc cggcgacggc agcaagctcg
      181 acggcgtgat cggcaaggtg ctcctgccct cactcctctc gccggtcatc gccatgatcg
      241 tggcggcaac ggcaacgcgg ctcatcttca tgatcaccgc gcgggtcgaa gacaagttcc
      301 gggagcgccg gttccgctgg ggtcaggtcg gcgcggcatc gctcgtctcc ctcgcgcacg
      361 gcaccaacga cgcccagaag acgatgggcg tcatcaccct ggccctcgtt gcggccgggt
      421 cggtgagctc caccgccgtc tacgtccccc tctgggtgaa gctgtcggcc gccctcgcga
      481 tcgctctggg cacctacctc ggtggctggc ggatcatccg cacgatgggc aagggtctgg
      541 tcgacatctc ctcaccccag ggcctcgcgg cggacgcctc gtcggccacg gtcatcctcg
      601 cctccagcca gctcggcttc gcgctctcga cgacccacgt cgccacgggc tcgatcatgg
      661 gcgtcggcat cggcaagaag ggtgcctcgg tgcgttggtc ggtggccggc cgcatggtcg
      721 ccgcctgggc catcacgttc ccggccgcgg ccgtcatggg agccgtgatg tattggatcg
      781 ccgccggaat cggtgggatc gtcggccccc tcgtcgtctt cctgctcctc gtgggcctct
      841 ccttctacat gtggaagcgc tcgcgggtga atgccgtgga ccacaagaac gtcaccgacg
      901 agtggcacga cggaagcaag ccggaagtcg cagtcgccgc ccacgacgtc atcgaccccc
      961 accacgagtt ggacgcgccg cagcccgccg gcccggccgc gcactgaccg gaagaggaac
     1021 gcatcatgaa catgattccg ctcgccctcg acggggcttg gaaggttctc gtcgcggggc
     1081 tgctcctcgg agccggcctg ccggtcctct acgcgttcgc ggtccgtggc ctggccatgg
     1141 ccaggggcgc cggggccatc atcggtgagc agacagccaa gccattcggc aaggtcatcg
     1201 cctgggtgct gttgctggtg atcctgctgg ccgtgtcgta cggcatcagc ttcatcgtcg
     1261 ccacggggat cggcaaggtc gtcaccttcg accacatcct cccctggttc gtcgacaagc
     1321 cgaagaagta gccctgagca ggcccctcat cccggggggg cggcccgagt tccgaccaac
     1381 ggcaaactga cggggtatgc cgcagattcc cctccccggg gtgtgtccgg ggaggggaag
     1441 tgcgggcacc tggccgaaac gttgcggtgg cacgccaggt gcccgcacac ccttgggaag
     1501 cggccttcaa ccccctcaag ttaacgcaag atgaacgatg aaagaacggc cgctagagtc
     1561 ccgtctgcgt aaccgcgtga tgcaggacgc acccgggact ttcgtctcgg ggatcagcac
     1621 aaccggcccc cgatcatggg gcgcgtgacc accgcaaccg tcattctcgt catggtcgtt
     1681 ctcacggccc tggccttcga cttcaccaat ggcttccacg acaccgggaa cgccatggct
     1741 gcgtccatcg ccacaggtgc gctgtccccc aagatggccg tcaccctagc cgcgactctc
     1801 gacctcgtcg gagcgttgat ctccgtcaag gtcgccctta ccatcaccaa cgccgtgatc
     1861 gacggcatcc agatgaagac cggcgttccc aacccgcaat tcatcggcag cgacaacggc
     1921 aagcagggca ccatcctgct catggtcgtt ctcgccggcc tcgtcggcgg catcgtctgg
     1981 aacctcctca cctggctgct cggcctgccg tcctcctcct cacacgccct cctcggcggg
     2041 ctggtcggcg cgaccatcgc gggattcggc ttctcccaag tcaactggct cggccactcg
     2101 acagagaagc tcgacggcgt cgtcggcaag gtgctcatcc cctcgctgat ctcccccgtc
     2161 atcgccttca tcgtcgcggc cgtcggcacc cggatcgtct tctcgctcac cagcaagatg
     2221 ggtgacaagt tccgcgagtc gcggttccgc tatggccagg tcggcgcggc ctcgatggtc
     2281 gccctcgccc acggcaccaa cgacgctcag aagacgatgg gtgtcatcac cctggctctc
     2341 atcgccgccg gtcaagtcga cgtctcgcag gagatctcga tcccgttctg ggtgaagctc
     2401 tccgccgccg tcgccatcgc cctgggcacc tacctcggtg gctggcgcat catgcggacc
     2461 ctcggcaagg gcatcgtcga catcaacgcc ccgcagggct tcgccggcga cacctcgacc
     2521 gcgtcggtca tcctcgcctc cagtggcctg ggcttcgccc tctcgacgac ccacgtcgcc
     2581 tccggctcca tcatgggtgc cggcgtcggc cgcaagggcg ccgaggtccg ttggtcggtc
     2641 gccggccgca tggccagcgc gtgggccatc accttcccgg ccgcaggtct ggtcggcgcg
     2701 gtgatgttct tcatcagcaa cctgatgccc cagctgctcg gcgccacggt cgtcatggcg
     2761 atcctcatcg ccgtgtcgct ctacatctgg atgcgctcgc gcaaggaccc ggtcgacaag
     2821 cacagcgttg gcaaggactg ggaagccgag ccggacgcgg cccacgttgc cgaagccgtc
     2881 ccccacgccc accactgacc ggaaagccga ctcatcatga acaacctcat cattgcgctt
     2941 gacggtgcgt ggaaggtcct gctcgcgggt ctgctcctcg gcgccggaat ccccaccctg
     3001 ttcgccatgg ccgtcaaggg tctggcgatg gccgagggcg gcgaggccgc tggaggcgca
     3061 tccccgcggg ccgcgggcaa gctcatggcc gcggtcttct tcttcatcat cctgacgctg
     3121 gtcgtctacg gcttggcctg gctcatcttc acgagccttg gcttcaaggt cggcttcagc
     3181 ggccccttcc cgacgttctc gaagtgaggt ttcaccacta atgtccccgc acactgagag
     3241 cgccgggcgt cgtctggtca actggctgcg cgccggtcgg cccgagggca tcgcccaggg
     3301 tgactacatc gccctgatgg gcgtgctgca ccgcaagctc acgaccgccg aggtcgagtc
     3361 catcgccgtg tcgctcgtcg agcgcgacta cgacgacgag ctcatcaccg aggacgagat
     3421 ccgcgccatg atcgacgaga aggtccacga agaggcgacc gaggacgaca tcgcccgcat
     3481 cgcgcacctc cttgaagagg cgggcgtcca cgtcgacccg cacgtgccgg gagcgacgca
     3541 ctgacgtcgt cggacgttca ctgaacaaca ctgtgtggcc gaccacctct ggtggtcggc
     3601 cacacagtct ttgctacccc ttcatttctt agccgctttg gcgtcgattg cactggcgac
     3661 aatcaccgca tggggagata ccgagggata ttgaggttcg cgctcccagg ctcgggggca
     3721 aggcccacca ccgcggttcc gctgatcgcc ctgctcggcc gacggctctc cgatcaggag
     3781 gtcacggatc tcgaggaaac gctggccgag cgcgcccggg cccgcggatg tgactcgttg
     3841 gccgaaatcg atctcgtcat agccatcacc ctgctcactg atgaactgcc gacgacccgg
     3901 gacattgatc gggttcgtga cgcgctcggg cgccgcggcc tcaccgtgca gcggcgagtg
     3961 ccccagccca cgtgacgggc tgggaacaga cgcgtcaggg gttcaggccg aggcgccggg
     4021 cgctgcgctg acgctgacgg ctggcgcgca accgccgcaa ccgcttgatg agcatggggt
     4081 cggtctcaag ggcggactgg ttgtcgatca gggcgttgag gacctggtag taccgcgtcg
     4141 ccgacatgtc gaacagttcc ttgatggcct gttctttggc gccggcgtac ttccaccact
     4201 gccgttcgaa ggccagcacc tcgcggtcgc ggtcgctcaa cggcgcatgc gccgcttcaa
     4261 cccgctctgc tgtgtctgcc atccgaaccc tccgctagtg actccctcgg caactataga
     4321 ggggaatgac atcgatgtca ttcccctcgg cgcgtcgcct tgcgattccc tatcgcctgt
     4381 gacacgagtg acggatgggg ctgccgcagc gccgtcaggg cagcagcagg gcccgcagcg
     4441 ccgcgacgtt cgaggctacg ccgagcggct cggcggcccg cagatcggct tcggttccgg
     4501 cgccccagag gacaccgatc cccgccatcc ccgctgcctg cgccgcccgc acgtccacga
     4561 ccgcgtcccc gacgtatgcc gccctcccgg gctctccgcc ggcccgctcg acggcgagca
     4621 gcaacggcgt ggggtcgggc ttgtggaccg tggtgtcctc catggtcacc aggacgggga
     4681 cgagatcggt caacccggtc agcgccaggg ctttggctgc cggtggatgc cgcttggacg
     4741 tcgccacggc cacccgcgcc ccttggctcg tgagctcgag gagcaactcc gccactcccg
     4801 cgtacccccg caccagccgc tcggtgttgg cttcgttcca cgtcgtgtat gccgcgaaca
     4861 acgcgtccgc gtgggcgggt gaagactccc ggaagcatcg cagcagcggc tggccgatcc
     4921 acgcccggat ccgggcctcg tcttcctccc ggcccaggac cgtgcggaag gcatgctggt
     4981 aggacgcaac gatgaggttg atcgtgtcca ccagcgtgcc gtccagatcg aagacgaccg
     5041 tgggccacgc caacccaccg cgacgtgcgg tcacgacgcg ggtccggcga gcaaaggggt
     5101 cccgatccgg aacacccggg ccagcgccgt cgccaggagc ttgccgtcag gatggcccgg
     5161 ccggtcatcg gcgaacacgt cgatgcggaa taccgcagtc cgcgccgtct tgctgatctc
     5221 gacaccttca cagcgcaaag cgtcgccctc gacaccagcc gagaggaact cggtatgcac
     5281 gtcctgggcg acggcgagcg tgccgtggct gttgctcact gcggcatgaa cgacatcgat
     5341 caaggccatc atcgcgccgc cgtgcgcagt gccaggcgcg ttgagcaggc ggtgggtgac
     5401 cggcatacgg gctcgggcgt aacccggccg gatgtcctcg aagacgatgc cgagcaagtc
     5461 ggccaccgga tcggtcgcga gataggcgaa cagctcgggg cttaccggct cattggactg
     5521 cgctggcagc tccgggcggg tcatgccccg accctatccg ccgtaagccg acgcgcgttc
     5581 cggtgccggg tggaccgccg cgggaaggtc cagaggctca gggaccccgg cccgcgggtg
     5641 gaagaactcg gtaaggcccg ccccgagggc ggcgccgacc agttgggcac cgatgaaccc
     5701 cgcgacgctt cccggcgcga tcccggagaa ggtgtcggtc gccgagcgag cgatcgtcac
     5761 ggccgggttc gcgaaggacg tggaggaggt gaagaagtat gccgccccga tccaggctgg
     5821 cacgagggcg gggcccaacc ggccttgacc ggagcgggtg agcccaccga cgagcgaaag
     5881 caggccggcg gtcgcgacga cctcgccgag ccacaggctg ggggacgagc ggactttcat
     5941 ggaggcctgc caggccggca ggtcgaacat gaggttgccg accgccgtcc cggccagccc
     6001 tccgacgatc tgggcgatga catagcggca cgcctcggcc gcgccgagtt cgcggcgcac
     6061 gagggcgacg agcgttacgg cggggttgaa atgagcccct gagatcggcc cgagcgccca
     6121 gatgagcacg gcgagggccg cgatggtggc cagcgcgttg accagcagtt gcaccgcgac
     6181 atcctgggtc aggcgggtgg ccatgatccc ggagccgacg acggtggcga cgagggcgcc
     6241 cgttccgaga gcctcggcca gggcccggcg agtgctcagc agcattgctg gccgggggt
//
LOCUS       DAOMZY010000211         6293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_11658_length_6293_cov_5.991824, whole genome shotgun sequence.
ACCESSION   DAOMZY010000211 DAOMZY010000000
VERSION     DAOMZY010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6293
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11658_length_6293_cov_5.991824"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /gene="treS"
                     /locus_tag="PLK64_11670"
     CDS             complement(<1..1376)
                     /gene="treS"
                     /locus_tag="PLK64_11670"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HPV80451.1"
                     /translation="MTSASTVPADQTTTSGLREDPDWFRTAVFYEVLVRAFADSTGSG
                     NGDFVGLIDKLDYLHWLGVDCLWIPPFYASPLRDGGYDIADYRSVLPEFGTIEDFREL
                     VAQAHARGIRIITDFVMNHTSDAHPWFQASRSDPDGPYGDYYVWSDTDTKYAGTRIIF
                     VDTESSNWSFDVVRRQHYWHRFFSHQPDLNFESPAVQAEMFDVVRFWMDMGIDGFRLD
                     AVPYLFEEEGHNGENHPKTHEFLAQLRAMVDREYPGRILLAEANQLPAEVVDYFGTEQ
                     DPECHMCFHFPVMPRIYYSLRSQTAAPIIDVLAATPAIPPGTQWGTFLRNHDELTLEM
                     VSDDERAAMYRWYAPDPRMRANVGIRRRLAPLLDNDRSQLELINALVLSLPGSPCLYY
                     GDEIGMGDNIWLPDRDCSRTPMQWNPDRNAGFSTADPGKLYLPVVSSLVFSYQAVNVE
                     SAQAAPASP"
     gene            complement(1465..>3456)
                     /locus_tag="PLK64_11675"
     CDS             complement(1465..>3456)
                     /locus_tag="PLK64_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HPV80452.1"
                     /translation="IGRIPVTDVLPSVDGGTRAAKSVVDEQFWIQARVFREGHDAVNA
                     TAVLTDPDGVDHAFPMTSVNVGLSLWNVQVSADQAGWWTYRVEGWSDPWETWVHDGTI
                     KVEAGIDEELMLEEGARVVEHALAALPDSAPASARTELRDVVTALRDPNRPPIARLRA
                     ATAPEVHAIFAAYPFREKVSPSAAYPLLVERELALFGAWYEIFPRSEGATKDPVTGTW
                     TSGTFRTAAKRLPAIAAMGFDVVYLTPIHPIGTTARKGKNNTLVALPGDPGSPYAIGS
                     PDGGHDALHPELGTFEDFDAFVAEAGRLGMEVAMDIALQCSPDHPYVATHPEWFTTRA
                     DGTIAYAENPPKKYQDIYPLNFDNDPVGAYAEMRRMIQVWIDHGVKIFRVDNPHTKPV
                     EFWQWLIADVARDHPEVIWLAEAFTRPAMMHTLGKAGFQQSYTYYAWRNAKWELEDYC
                     RELAGDAAAYMRPSFWPTTHDILTPFMQYGGPTAWKLRAAIAATLVPTYGIYTGYELM
                     ESVARPGVEEQIDNEKYEFKDRRWADYEPGGPKDGQSLAGYLTMLNAARKAHPALHWM
                     RNLHFHHAPDDAIMCFSKSRVLPDGRRDTVIVVANLDPHSTRETQVDLDLPALGLPWG
                     SALRVHDLVTDAVWDWGQYNYVRLGADTEPVHILEVLSP"
     assembly_gap    3457..3556
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3850..>6293
                     /gene="glgP"
                     /locus_tag="PLK64_11680"
     CDS             3850..>6293
                     /gene="glgP"
                     /locus_tag="PLK64_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPV80453.1"
                     /translation="MKAIRRFNVRTVLPEPIAALGDLALNLHWSWHPSTRDLFKSIDP
                     VRWDECHQDPTKMLARLSAAELATLANDAEFVARVDVAKAALDHYLEAPRWYQEWAAA
                     QVTEESPGAPRAIAYFSPEFGITHVLPQYSGGLGILAGDHLKSASDLGVPIVGVGLFY
                     KTGYFKQSLNELGWQQEAYPVLDPDGLPLALLREDDGTPTVIEIRLPGSRVLKAHVWR
                     AQVGRVPLLLLDSDVTGNDDATRAITEALYGGGGDRRLEQELLLGIGGVRALRAWSRI
                     TGAPEPDVYHTNEGHAGFLGVERISELVRDHRLTFDEALEAVRASTVFTTHTPVPAGI
                     DRFSADRIHIYFGGDCAMTGIPLDRLLAMGAETYEGGEAGMFNMAVMGLRLAQRANGV
                     SLLHGQVSRDMFDGLWPGFDHEEVPIGSITNGVHAPTWVDRRMLELAETHLGAAVLDG
                     EGGWEQIRNVNWDQMWAVKRELRQQLVADARRRVRSSWLERGASAAELKWTDEILDPD
                     VLTIGFARRVPTYKRLTLMLRDPARLKKILLDKKRPVQLVIAGKSHPADDLGKRLIQQ
                     MVQFADDPEVRHRIVFLPNYDIEMAQTLYPGCDVWLNNPLRPFEACGTSGMKAALNGG
                     LNLSILDGWWDEWYDGQNGWAIPTADGVEDPERRDDIEASALYDLIENSVRVHFYDQN
                     ENGLPLHWMELLLHTLETLGPKVLATRMVRDYVTELYGPAARAGWSLDGPKFTGAREL
                     AAYKAKVRASWPSVRVEHVESSGVSDSPQVGDELTVRAYVDLGTLRPDEVRVQVVYGP
                     SSDRDDTIRRPTVVKL"
BASE COUNT          951 a         2046 c         2184 g         1012 t
ORIGIN      
        1 ggcgacgccg gcgcggcctg ggccgactcc acgttgaccg cctggtaact gaacacgagc
       61 gaggacacga ccgggaggta gagcttgccg gggtcggcgg tggagaagcc ggcattgcgg
      121 tcggggttcc actgcatggg cgtgcgcgaa cagtcccggt cgggcagcca gatgttgtcg
      181 cccatcccga tctcatcgcc gtaatagaga cacggtgacc cgggcagcga gagcaccaac
      241 gcgttgatca actcgagttg cgaacggtcg ttgtccagca ggggcgccaa gcgacgacgg
      301 atgccgacgt tggcccgcat ccgggggtcg ggggcgtacc agcggtacat cgcggcgcgc
      361 tcgtcgtcgc tgaccatctc gagggtgagc tcgtcgtggt tgcgcaggaa cgtgccccac
      421 tgggtgcccg gcgggatcgc cggtgtcgcg gcgaggacat cgatgatcgg ggccgcggtc
      481 tggctgcgca gggagtagta aatgcgcggc atgacgggga agtggaagca catgtggcac
      541 tcggggtcct gctcggtccc gaagtagtcg acgacctccg ccggcaactg gttggcctcg
      601 gccagcagga tccgcccggg gtattcgcgg tcgaccatgg ctcgcagctg ggcgaggaac
      661 tcgtgggtct tggggtggtt ctcgccgttg tgtccctcct cctcgaagag gtagggcacg
      721 gcatcgaggc ggaacccgtc gatccccatg tccatccaga accgcacgac gtcgaacatc
      781 tcggcctgaa cagccgggct ctcgaagttg aggtcaggct ggtgggagaa gaaccggtgc
      841 cagtagtgct ggcgccggac cacgtcgaag gaccagttcg aggactcggt gtcgacgaag
      901 atgatgcggg tcccggcata tttcgtgtcg gtgtccgacc acacgtagta gtcgccgtag
      961 ggcccgtccg ggtcgctgcg gctggcctgg aaccacgggt gcgcatcgct cgtgtggttc
     1021 atgacgaagt cggtgatgat ccgtatgccg cgcgcgtgcg cctgcgcgac caactcgcgg
     1081 aagtcctcga tcgtcccgaa ctcgggcagc accgagcggt agtcggcgat gtcgtaaccg
     1141 ccatcacgaa gcggcgaggc gtagaacggc gggatccaca ggcagtcgac gccgagccag
     1201 tgcaggtagt cgagtttgtc gatgagcccg acaaagtcgc cgttccccga tccggtggag
     1261 tcggcgaagg cccgcacgag gacctcgtag aacaccgcgg tccggaacca atcgggatcc
     1321 tcacgcagtc ccgacgttgt ggtctggtcc gcaggaacgg tggacgcgct ggtcacgact
     1381 gagacgctcc ttctgctggt gctgagatcg ggccggatgg tgctggcatg gccgagggcg
     1441 ctgccttcgc cgagggtgcc gccatcaggg cgagaggact tccaggatgt gcaccggttc
     1501 ggtgtcggca ccgagccgga cgtagttgta ctggccccag tcccagacgg cgtcggtgac
     1561 caggtcgtgc acgcgcaggg cggagcccca cggcagcccg agcgcgggca ggtcgaggtc
     1621 gacctgggtc tcgcgcgtgg agtgcgggtc gaggttggcc acgacgatga cggtgtcccg
     1681 ccgaccgtcg ggcagcaccc ggctcttgga gaagcacatg atcgcgtcgt cgggggcgtg
     1741 gtggaagtgc aggttgcgca tccaatgcag ggccgggtgg gccttgcggg ccgcgttgag
     1801 catggtgagg taaccggcca gcgactgccc gtccttgggc ccgccgggct cgtagtcggc
     1861 ccagcggcgg tccttgaact cgtacttctc gttgtcgatc tgctcttcga cgccaggccg
     1921 ggccacgctc tccatcagct cgtaaccggt gtagatgcca taggtcggca cgagcgtcgc
     1981 ggcgatggct gcccgcagct tccaggcggt cggcccgccg tattgcatga agggggtcag
     2041 gatgtcgtgg gtggtcggcc agaacgacgg acgcatgtat gccgcggcgt caccggcgag
     2101 ctcgcggcag tagtcctcca gctcccactt ggcgttgcgc caggcgtaat acgtgtagga
     2161 ctgctggaag cccgccttgc ccagcgtgtg catcatcgcg ggccgggtga aggcctctgc
     2221 cagccagatg acctccgggt ggtcgcgggc cacgtcggcg atgagccact gccagaactc
     2281 gaccggcttg gtgtgcgggt tgtcgacccg gaagatcttg accccgtggt cgatccagac
     2341 ctggatcatc cgacgcatct cggcgtaggc gccgaccggg tcgttgtcga agttcagcgg
     2401 gtagatgtcc tgatacttct tcggcgggtt ctcggcgtag gcgatggtgc cgtcggcgcg
     2461 ggtcgtgaac cactccgggt gcgtcgcgac gtaggggtgg tcgggggagc actgcagggc
     2521 gatgtccatg gccacctcca tcccgagccg gccggcttcg gccacgaagg cgtcgaagtc
     2581 ctcgaacgtc cccagctcgg ggtgcagcgc gtcgtgcccg ccatcgggcg agccgatggc
     2641 gtaggggctg cccggatcgc ccggaagcgc gacgagcgtg ttgttcttgc ccttgcgcgc
     2701 ggtcgtgccg atcgggtgga tcggggtgag gtagacgacg tcgaaaccca tcgccgcgat
     2761 cgcgggcagc cgcttggccg cggtccggaa cgtccccgac gtccacgtcc ccgtgacggg
     2821 gtccttcgtc gcgccttccg agcgcgggaa gatctcgtac cacgcaccga acagcgccag
     2881 ctcgcgctcc acgagcaagg ggtatgccgc cgacgggctc accttctcgc ggaaggggta
     2941 tgccgcgaag atcgcatgca cttctggcgc cgtcgctgcc cgaagtcggg cgatcggcgg
     3001 gcggttcggg tcgcgcagcg cggtgacgac atcgcgcaac tccgtgcggg cgctcgccgg
     3061 cgcgctgtcg ggcagggcgg ccagggcgtg ctcgacgacg cgggcgccct cctcgagcat
     3121 gagctcctcg tcgatgccgg cctcgacctt gatggtgccg tcgtggaccc aggtctccca
     3181 gggatcggac cacccttcga cgcggtaggt ccaccagccg gcctggtcgg cgctgacctg
     3241 gacgttccac agcgacaggc cgacgttgac gctcgtcatc gggaaggcgt ggtcgacccc
     3301 gtcggggtcg gtgaggacag cggtcgcgtt gaccgcgtcg tggccctcgc ggaagaccct
     3361 cgcctggatc cagaactgct cgtcgacgac cgacttggcc gctcgggtgc cgccgtcgac
     3421 cgacggcaag acgtcagtga ctgggatccg accgatnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnngtcg ccacgggcgg ttccggggct gcggtggcgg cgggcggcat
     3601 acccgcgccg gggccgcgtg gctcgcgcgg cgaggtgccc gcagcggggc ggttccgggg
     3661 ggtgcgcttg cgggcagagg aggcgctcac gaaggcgacg ctactcggtc gagtcggtcg
     3721 tcgtctgccc ccatggcccc gcaacgatca cattctcatc gttcggatgt aaccgcttgc
     3781 gcactttgtc cgttcagacc catggacggt ggcagtaaac cggcgagtag ccccctatag
     3841 tcgctccccg tgaaggccat ccgacgcttc aacgtccgca ccgtcctgcc cgagcccatt
     3901 gctgcgctcg gtgacctagc actgaacctg cactggtcat ggcaccccag cacccgggat
     3961 ctcttcaaga gcatcgaccc ggtccgttgg gatgagtgcc accaggaccc caccaagatg
     4021 cttgcccgcc tctcggcggc cgagctggcc acactcgcca acgatgccga gttcgtcgca
     4081 cgcgtcgacg tcgccaaggc ggcgctcgac cattacctcg aggctcctcg ctggtaccag
     4141 gaatgggccg cggcccaggt aaccgaggag tcgccggggg caccgcgtgc catcgcctac
     4201 ttcagccccg agttcggcat cacccacgtg ctgccgcagt actccggcgg tctgggcatc
     4261 cttgctggtg accacctcaa gtcggcgtcc gacctgggtg tgccgatcgt cggcgtcggc
     4321 ctgttctaca agaccggcta cttcaagcag tcgctcaacg agttgggctg gcagcaggag
     4381 gcctacccgg tcctggaccc cgacggtctg ccgctggcgc tgctgcgtga agacgacggc
     4441 accccgacgg tcatcgagat ccggctgccc ggctcccggg tgctcaaggc ccacgtctgg
     4501 cgggcccagg tcggtcgcgt gccgctgctg ctgctggact cggacgtcac cgggaacgac
     4561 gacgccaccc gcgccatcac cgaagccctc tacggcggcg gcggtgaccg ccgcctcgag
     4621 caggagttgc tcctgggcat cggtggcgtg cgcgcgctgc gtgcgtggtc gcggatcacc
     4681 ggggcacccg agcccgacgt ttaccacacg aacgagggcc acgccggctt cctcggggtc
     4741 gagcggatct ctgagctcgt ccgcgaccac cggttgacct tcgacgaggc gctcgaggcc
     4801 gtgcgtgcct cgacggtctt caccacccac acgccggtgc cggctggcat cgaccgcttc
     4861 tccgccgacc ggatccacat ctacttcggc ggcgactgcg cgatgaccgg catccccctg
     4921 gaccgcttgc tggccatggg cgccgagacc tacgagggcg gcgaggcggg catgttcaac
     4981 atggccgtca tggggctgcg gttggcccag cgggccaacg gcgtctcgtt gctgcacggc
     5041 caggtcagcc gcgacatgtt cgacggtctg tggccgggct tcgaccacga agaggtgccg
     5101 atcggcagca tcaccaacgg tgtccacgcc ccgacgtggg tcgaccgccg gatgcttgag
     5161 ctggctgaga cgcacctggg cgctgcagtc ctcgacggag agggcggctg ggagcagatc
     5221 cgcaacgtca actgggacca gatgtgggca gtcaagcgcg agctgcgtca gcagttggtc
     5281 gccgacgccc ggcgtcgggt tcggtcctcg tggctcgagc gcggcgcgag cgcggccgag
     5341 ctcaagtgga ccgacgagat cctcgacccc gacgtgctga ccatcggttt cgcccggcgg
     5401 gtgccgacct acaagcggct caccctcatg ctgcgcgacc cggcccggct caagaagatc
     5461 ctgctggaca agaagcgtcc cgtgcagttg gtcatcgctg gcaagtcgca cccggccgac
     5521 gacctgggca agcggctcat ccagcagatg gtgcagttcg ccgacgaccc cgaggttcgt
     5581 caccgcatcg tcttcctgcc caactacgac atcgagatgg cgcagaccct ctacccgggc
     5641 tgcgacgttt ggttgaacaa cccgctgcgc ccgttcgagg cgtgtgggac gtcgggcatg
     5701 aaggcggcgc tcaacggcgg gctcaacctc tcgatcctcg acgggtggtg ggacgagtgg
     5761 tacgacggcc agaacggttg ggccatcccg accgccgacg gcgtcgaaga ccccgagcgt
     5821 cgtgacgaca tcgaggcctc cgcgctctac gacctcatcg agaacagcgt gcgggtccac
     5881 ttctacgacc agaacgagaa cggtctgccc ctgcactgga tggagctgct cctgcacacg
     5941 ctggagaccc tcggccccaa ggtgctggcg acccggatgg tccgcgacta cgtcaccgag
     6001 ctctacggcc cggcggcgcg tgcgggctgg tccctggacg gtccgaagtt caccggcgct
     6061 cgcgagctcg cggcatacaa ggcgaaggtc cgtgcgtcgt ggccgtcggt ccgcgtcgag
     6121 cacgtggagt cctccggcgt gtccgactcg ccgcaggtcg gcgacgagct gaccgtgcgg
     6181 gcctacgtcg acctcggcac gctgcggccc gacgaggtgc gggtgcaggt cgtctacggc
     6241 ccgtcctcgg accgcgacga cacgatccgg cggcccacgg tcgtcaagct caa
//
LOCUS       DAOMZY010000212         6269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_11708_length_6269_cov_10.536209, whole genome shotgun
            sequence.
ACCESSION   DAOMZY010000212 DAOMZY010000000
VERSION     DAOMZY010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11708_length_6269_cov_10.536209"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="PLK64_11685"
     CDS             complement(<1..965)
                     /locus_tag="PLK64_11685"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPV80454.1"
                     /translation="MSESRLEFLLEKLDEIWNVYGSDKVGKGGAFERLMASYLRTAPE
                     FSDRFDEVYLWQDWPERGAMHDHGIDIVAKDLHTGGWCAVQCKFYNPQHHVSKADIDS
                     FLAASAKKQFTGRIIVSTAKDWGPTAESQLDGLTVPVTRIGVNDLLESQVDWGQIDWQ
                     EPQTYLPLTGKKQLRPHQSAAKDAAVTGLATHDRGQLIMACGTGKTFTSLKIAEQLAT
                     DNKAKATTVLFLVPSIQLVNQTFREWAQESEIPFRAFAVCSDVKVGKSGPRPKDDPED
                     IAVHDLIIPATTDTKALAAKVREAHEDDRFTVIFSTYQSIDVVAQA"
     gene            1128..3890
                     /locus_tag="PLK64_11690"
     CDS             1128..3890
                     /locus_tag="PLK64_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80455.1"
                     /translation="MGGLSTGGGSSAVAASAHAGCERFRYTDPLVIGMTRRALAARLG
                     HPDTSAGIPEARWMRAMTFESLVRHERFVSELLTTTVGRLSLVRPEAVRRANGRVSVD
                     ATATALQQAHLKSVHEGVATMVTNLAVPFVGMEDVAGATPVKPDFAIVAPRHRLGADG
                     TEASGDGRPIGSWLVMGDAKDYERVRSRIDDQRMLKGFLQVALGAASAQAWSKVPVGM
                     TVHSWGALAVPRNAYLQPEAVVECLDDHRSEVIDRVRERQQVLDSRGDTVVADHELPG
                     WVSHLQAAFDPASCPSCAMFSHCRAEVRASTEPVSRLVELGIPAQQRPGLIGLVDGTG
                     TVAVSAPASSVASLTATLTGMPQHTGRLRTDPVGMAGTVNVVIAKSDAAALGVLGIAL
                     RDGEDPSAEGWTFTVFEDPQASTTRLAVMDLIGDALDRALTATGAGDGSKRDPIHLVM
                     PDAATGDVLVSIADSLAGVEISRLRWERDVQVGRKPLTFNGEPATIPAALTANQRLAV
                     SFLLEADRSRAMALRSALIDIRRVLAAHVVPGGPRPDALRLDYLVTWATATEALDHRA
                     VSDEVAESDSTPGARLSNRRSDTIHAVSRRGRAVGEGYDAHAYGALVRDELEYKARVL
                     DRAAQALSSFQVSRLRDVHRALEADAQTVWLRRWALHASDLVRFGRVPWWWRNDQVDQ
                     LDTDSRLEAQLMALGNPQAARELATTAGTREVAIATVTQLSPCRLRIASRRIKDGSLV
                     HVAHTPAGPIVDWETTTLKVQTGSFKFGEQWLGPLTADDQTAEDGTLIWEPSMPVIFP
                     VGSELIVIDGGWFSTFKSGREVAVKRPTLDQTSSPKPICLEGDYAAAPMAHQWCCRPH
                     EEAEAEFSDDLAARRERGELNPQVWPPVIDEDHFDTVADGAPTETDAQAVTAQPPTGL
                     TMDDLE"
     gene            3898..5421
                     /locus_tag="PLK64_11695"
     CDS             3898..5421
                     /locus_tag="PLK64_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80456.1"
                     /translation="MPFTFSPPTVATSGAVHHGAAAMRNVDVTTRAIGALQDALDGAE
                     PYERLLLKAAAGAGKSFALRRLVHEAISHPDVSRVGVTAFANKQVFPLAAALGKELGA
                     SRVCLAVAPERLKDLPAGLSDVCTVAVTTKEVPGDAEVILGTTHKLQGTTPWLLKELG
                     PAANGDRFFDVLFVDEAWQLPLHRYRRIDRFAPLVVGVGDVGQLPPLDSSHNPWRGDA
                     RYNPYRAWPTAYEADERTWAAELPAVWRPTARQLGLWRAFYSDWESLDCVAAEGDRSI
                     ELGHGGLGDVARTVWDQVATGTPTLLEIADLADPEAPDVDLPLMNQLETLLNELIASS
                     FAVRQAVYDQHGMPNGETMEATVTTPGSDPLIVVLATRNQAVDDAQDVVDRLVAKHDL
                     PDGVLIASTVDSWQGQTNGITVAIHPLSGADALDEFNSSFGRLAVTCTRATHGLLLLS
                     RAGLDDLLALAPARPGTPLGEPGNRQLPRQTHQRILATFARGVVSAFETGVAEETYG"
     gene            5474..>6269
                     /locus_tag="PLK64_11700"
     CDS             5474..>6269
                     /locus_tag="PLK64_11700"
                     /inference="COORDINATES: protein motif:HMM:NF039405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6308 family protein"
                     /protein_id="HPV80457.1"
                     /translation="MERSATLSDRFTSVPDGWDKATADQVQYAMDQVWTALNHEDVVT
                     RVERYYRKDGNFAGATFLGLDPVDPYRFTSADLLSLNLLSVVAQPVAIRRLLEPSRER
                     GHLLRLLSEENLPLDADLAMADDGQLVAMADLHEALKRVLSPADVRNPNPWVTASKLC
                     ARKRPDLFPVRDNVVVKYLGLDRPKGNYQVDWQVFRHVIQDNAIRRRLDAIVDEASRS
                     PGVDVGHPNWRLRHLDVVLWMHARPQDARSRASTSPAPTTLAPWGSR"
BASE COUNT         1003 a         2057 c         2150 g         1059 t
ORIGIN      
        1 gcctgggcga cgacgtcgat cgactggtag gtcgagaaga tcaccgtgaa gcgatcgtct
       61 tcgtgcgctt cgcggacctt ggcagccagc gccttggtgt ccgtcgtcgc cgggatgatg
      121 aggtcgtgga ctgcgatgtc ctcggggtcg tccttgggtc gtggcccgct cttgccgacc
      181 ttgacgtcag agcagacggc gaaggcacgg aacgggatct cgctctcctg ggcccactcc
      241 cggaaggtct ggttgaccag ctggatgctc gggacgagga agaggaccgt cgtcgccttc
      301 gccttgttgt ccgtcgcgag ctgctcggcg atcttgaggc tcgtgaaggt cttgccggtg
      361 ccacaggcca tgatcagctg cccgcggtca tgcgtcgcaa ggccggtgac ggcggcgtcc
      421 ttcgccgcgc tctggtgagg gcggagctgc ttcttgccgg tgaggggcag gtaggtctgg
      481 ggctcctgcc agtcgatctg gccccagtcg acctggctct cgaggaggtc gttgacgccg
      541 atccgcgtca cggggacggt gaggccgtcg agctggctct cggccgtcgg gccccagtcc
      601 ttcgcggtag agacgatgat gcgcccggtg aactgcttct tggccgaggc ggcgaggaaa
      661 gagtcgatgt cggccttcga cacgtggtgc tgcgggttgt agaacttgca ctggaccgcg
      721 caccagcctc cggtgtggag gtccttggcg acgatgtcga tgccgtggtc gtgcatggca
      781 ccgcgttcgg gccagtcctg ccagaggtag acctcgtcga accggtcgga gaactccggt
      841 gcggtgcgga ggtaactggc catgagccgc tcaaaggcgc cgcccttgcc gaccttgtcg
      901 gacccgtaga cgttccagat ctcgtccagc ttttcgagca ggaactccaa ccgtgactcc
      961 gacaccccgt gccctttcgt caatatcctc ggccctttgt gtcgacacca tagccacggc
     1021 cttgtccggt cgagatgggt ggtgccgaga aggtgacaaa cagcgggaaa ggcagcgtga
     1081 attgcctatg gttcagggga cggagtccga atagggagtg acggcacgtg ggtggtttga
     1141 gtaccggtgg gggcagcagt gcggtggcgg cgtccgccca cgccggatgc gagcgcttcc
     1201 gctacaccga ccccttggtc atcgggatga cccggcgtgc gctggccgcg aggctcgggc
     1261 accccgacac ctcggccggc atccccgagg cccggtggat gcgggcgatg acgtttgagt
     1321 ccttggtccg gcacgagcgg ttcgtgtccg agctgctcac caccacggtc ggtcggttgt
     1381 ccttggtgcg cccagaggcg gtgcggcgtg ccaatgggcg ggtctcggtc gacgcgacgg
     1441 cgacagcgct ccagcaggct cacctcaagt cagtgcacga aggggtggcg acgatggtca
     1501 ccaatctcgc tgtcccgttc gtcgggatgg aggacgtcgc cggtgcgacc ccggtcaagc
     1561 ccgacttcgc catcgttgcg ccacggcatc ggctgggggc cgatggaacg gaggcctccg
     1621 gcgacggtcg gcccatcggt tcgtggctcg tcatgggtga tgcgaaggac tatgagcgtg
     1681 ttcggtcgcg gatcgatgac caacgcatgc tcaaggggtt tctccaggtc gccctcggtg
     1741 ctgcctctgc gcaggcctgg agcaaggttc cggtggggat gacggtgcat tcgtggggtg
     1801 cgctcgcggt cccccggaac gcctacctcc agcccgaggc tgtcgtcgag tgccttgatg
     1861 accaccggtc ggaggtgatt gaccgggtcc gggaacggca gcaggtcctg gacagcaggg
     1921 gagacaccgt cgtcgccgac cacgaactgc cggggtgggt gtcccacctg caggcggcgt
     1981 ttgaccccgc ctcctgtcct tcctgcgcca tgttctccca ctgccgcgca gaggtgcgtg
     2041 cttccacgga accggtttcc cgcttggtcg agctcggcat tcccgcgcag cagcggccgg
     2101 ggctcatcgg gttggtcgac ggaaccggaa cggtcgcggt atccgcaccg gcgagcagcg
     2161 tggcgtcgtt gacggccacc ctgaccggca tgccgcagca caccgggcga ctgcgaaccg
     2221 atccggtcgg gatggcggga actgtcaacg tcgtcatcgc caagtcggat gccgctgccc
     2281 tcggtgtgct cgggatcgcc cttcgcgacg gagaggaccc atcggccgag ggatggacct
     2341 tcactgtgtt cgaggacccc caggcatcga cgacgcgtct ggccgtgatg gacctcatcg
     2401 gcgacgccct ggacagggcc ctgactgcga ccggcgccgg cgacgggtcg aagcgcgacc
     2461 ccatccacct cgtgatgccg gatgccgcga cgggcgacgt tctggtctcc atcgccgatt
     2521 cgctggcagg cgtggagatc agtcggcttc gctgggagcg tgacgtccag gtgggacgaa
     2581 agcccctcac gttcaacggc gagcccgcca cgattcctgc ggcgctgacg gccaaccaac
     2641 ggctcgcggt gagcttcctg ctcgaagcgg acaggtcgcg cgccatggcg ttgaggagcg
     2701 cgctcatcga catcagacga gtcctggccg cgcacgtggt gcccggtggg cctcgcccgg
     2761 atgcgttacg cctcgactac ctggtgacgt gggcgacggc gaccgaagca ttggatcacc
     2821 gtgccgtgtc tgacgaggtc gcggagtccg actcgacccc gggcgcgcgg ctgtcgaacc
     2881 ggcgctctga cacgatccat gcggtcagtc gccgtggccg tgccgtaggc gaggggtacg
     2941 acgcgcacgc ctacggagcg ctggtacgag acgaactcga gtacaaggcg cgtgtcctcg
     3001 accgcgccgc ccaggccctc tcctccttcc aggtgtcacg actgcgcgac gtgcaccggg
     3061 cgctggaagc cgacgctcag accgtgtggc tgcgccggtg ggcgctgcac gcatcggacc
     3121 tcgtgcgatt cgggcgggtg ccgtggtggt ggcgcaacga ccaggtggac cagctcgaca
     3181 ccgactcgcg actcgaggcc caattgatgg cactgggcaa tccgcaggcc gcccgagagc
     3241 tggccacgac cgccggaacc cgcgaggtag cgatcgccac ggtgacgcag ctgtctccgt
     3301 gccgcctgcg gatcgcttct cgccgtatca aggacgggtc cttggtccac gtggcgcaca
     3361 cgccggcggg gcccatcgtc gactgggaga ccacgacgct caaagtacag acgggtagct
     3421 tcaagttcgg agagcagtgg ctcgggccgc tgaccgcgga tgatcagacg gctgaggacg
     3481 gcacgctgat ctgggaaccg tcgatgcccg tgatcttccc cgtcggctcc gagctcatcg
     3541 tcatcgacgg tgggtggttc tcgacgttca agtcaggccg agaggtggcc gtgaaacgac
     3601 cgacgctgga ccagaccagt tcaccgaagc ccatatgcct cgagggcgac tacgccgcgg
     3661 ctccgatggc ccaccagtgg tgctgtagac cccatgagga ggcggaggca gagttctccg
     3721 acgatttggc ggctcgccgc gagcgcggcg agctgaatcc tcaggtgtgg ccaccagtca
     3781 tcgacgagga ccacttcgac accgtggcgg acggcgcccc gaccgagacc gacgcacagg
     3841 ccgtcactgc ccaacctccc accggcctga ccatggacga tctggagtag gtgacagatg
     3901 cccttcacgt tctcgcctcc cacggtcgcc acttcgggtg cggtgcacca cggcgcagcc
     3961 gcgatgcgca atgtcgacgt caccaccagg gccatcggtg ccctccaaga tgctctggac
     4021 ggtgcggagc cgtacgagcg gctcctcctc aaggcagctg ctggggcggg gaagtccttc
     4081 gccctgcgcc gactggtcca cgaggcgatc tcgcatccgg acgtgtctcg cgtgggtgtg
     4141 acggccttcg ccaacaagca ggtcttcccg cttgcggccg ccctgggaaa ggaacttggc
     4201 gccagtcggg tgtgcctggc tgtcgcgccg gagcggctga aggacctgcc agccggcctg
     4261 agcgacgtct gcacggtggc ggtgaccaca aaggaggttc caggggatgc cgaggtcatc
     4321 ctcggcacga cacacaagtt gcaggggacg acaccctggc tcctcaaaga gcttggtcct
     4381 gctgccaacg gtgaccggtt cttcgacgtc ctgttcgttg atgaggcctg gcaactaccc
     4441 ctccatcgat accggcggat cgacaggttc gcgcctttgg tcgttggggt cggggacgtg
     4501 gggcagctcc cgcccctcga ctcgtcgcac aacccctggc gcggggacgc tcgctacaac
     4561 ccctacaggg cgtggccgac cgcctacgaa gcagatgagc ggacgtgggc ggccgagctc
     4621 ccggcggtgt ggcgaccgac ggcccgtcaa ctcggtctgt ggcgagcctt ctactcggac
     4681 tgggagagcc tggattgcgt ggctgccgag ggcgaccgat cgatcgagct gggccacggt
     4741 gggcttggtg acgtggcccg aacggtgtgg gaccaggtcg ccacgggcac accgaccttg
     4801 ctcgagatcg ccgacctggc ggaccccgag gcgcccgacg tggaccttcc gctgatgaac
     4861 cagctcgaaa cactgctcaa cgagctcatc gcaagctcat ttgccgtccg acaagcggtg
     4921 tacgaccagc acgggatgcc gaacggggag acgatggagg ccacggtgac aacaccgggc
     4981 tcagaccccc tgatcgtcgt gctggcgacg cgaaaccagg ctgtcgatga cgctcaggac
     5041 gtcgtcgacc ggctcgtggc caagcacgac ctacccgacg gggtgctcat cgcgtcgacc
     5101 gtcgactcgt ggcaggggca gaccaacgga atcaccgtgg ccatccaccc gctgtccggt
     5161 gcggatgccc tcgacgagtt caactcgtcc ttcggtcgcc tggcagtgac gtgcacgcgg
     5221 gcgactcatg gcctgctgct gctctccaga gccggcctcg acgacctgct tgcgctcgcg
     5281 ccagcacggc cggggacgcc gctcggtgag cccggcaacc gacagctccc gcggcagacg
     5341 caccagcgca tcctcgccac cttcgccagg ggtgtcgtct ccgcgttcga gaccggcgtc
     5401 gccgaggaga cctatgggtg acggcagcgt tgagacccca cggcccggag gggagcgtca
     5461 tctaggtccc gacatggagc ggagcgccac attgagtgat cgattcacgt ctgtcccgga
     5521 tggctgggac aaggcgactg ccgaccaggt gcagtacgcc atggatcagg tctggacggc
     5581 cctcaatcac gaggacgtcg tgacgcgcgt cgagaggtac taccgaaagg acggcaactt
     5641 cgctggcgcc accttcttgg gcctcgaccc cgtcgacccc tatcgattca cgtcggccga
     5701 cctcctgtcg ctcaacctgc tcagcgtggt tgcgcaaccg gtcgcaatcc gccgcttgct
     5761 cgagccgtcc cgcgaacggg gccacctcct acgtctgctg agcgaggaga accttccgct
     5821 cgatgcggac ctggcgatgg ccgacgacgg tcagcttgtg gcgatggctg atttacatga
     5881 agccttgaag cgcgtcttgt ctcccgcaga cgtcaggaac ccgaacccat gggtcaccgc
     5941 ctccaagctc tgcgcccgca agcgtccgga cctcttccca gtgcgggaca acgtggtggt
     6001 caagtacctc ggcctggatc ggccgaaggg caactaccaa gtcgactggc aggtgttccg
     6061 ccacgtcatt caggacaacg ccatccggcg acggttggat gcgatcgtcg atgaggcgtc
     6121 gcgaagtccc ggcgttgacg ttggccaccc gaactggcga ctccggcacc tcgacgtcgt
     6181 cctgtggatg cacgctcggc cacaggacgc gcgaagccgg gcgtcaacaa gcccggcccc
     6241 gacgaccctg gccccctggg ggagtcggg
//
LOCUS       DAOMZY010000213         6253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_11750_length_6253_cov_5.256857, whole genome shotgun sequence.
ACCESSION   DAOMZY010000213 DAOMZY010000000
VERSION     DAOMZY010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11750_length_6253_cov_5.256857"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PLK64_11705"
     CDS             complement(<1..460)
                     /locus_tag="PLK64_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80458.1"
                     /translation="MVHPTEPLIDLTSNRSDELGHRFTQKGAARACGVALNTIKRRRA
                     AGAFPNAISDDNGVWRIPLADLLAAGLNPGHPTDPEPAPEATDTATDLEVRLAVTQVQ
                     LVERERAHTAEQAAAQREADALRAQLRQIEAARDQAADHTAQIRSQTRDAL"
     gene            977..1414
                     /gene="mobC"
                     /locus_tag="PLK64_11710"
     CDS             977..1414
                     /gene="mobC"
                     /locus_tag="PLK64_11710"
                     /inference="COORDINATES: protein motif:HMM:NF017524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HPV80459.1"
                     /translation="MEQVVSPPLPKGDGGRGTSVTVRLTAEELAAVKRAAAAAGRRQH
                     SRWAREQLLAATELWDRDEPRSVAEAEARLREALRSSGVALARSELARIGSNLNQAVR
                     AANTAALSAARTGQAVDVAGLTAAVEAAREQLARLADDGEVLA"
     gene            1411..2262
                     /locus_tag="PLK64_11715"
     CDS             1411..2262
                     /locus_tag="PLK64_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80460.1"
                     /translation="MIAKITRGGSAGGLAAYLHGPGAGTEHAVEVDGQRVAGGMVVAS
                     NVPGVQVGDLDGRRWGRWLDRAAASRPEIKGPVWHCSLNLAPGDRALTSLEWADAASE
                     FVARMGIPASHPWAAVQHDDRGIHIAVSRVDDTGRVWHARHDRRTAQKARQAVETRYQ
                     LAAAPTQKTDYGAVLPAPPAVGVHVVGSDTWRVRGHDGRTVTDRLGRDGRWRLVTNGM
                     RTLRIAVARDQDPDTLDLAARIARQRAAFSTVHPPNQTTPDPTPQRSPYRPPPTRDPG
                     RGSGRGR"
     gene            complement(2576..4180)
                     /locus_tag="PLK64_11720"
     CDS             complement(2576..4180)
                     /locus_tag="PLK64_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPV80461.1"
                     /translation="MTFTPRLNRFAAWGTEGPDPVSAAERELIAQVARHRFGVDHAPR
                     TPVDPATIELREPRMVVPDSLGAVVTAARADRLAHAWGKSFPDLARAFCGLAPHTPDL
                     VAYPRDETDIERILEWASATSVAVIPYGGGSSVVGGVTPDIGDDHTGSISLDLQRLDQ
                     VLEVDPVSRAARVQAGVLGPAFADQLRPHGLALRHYPQSYQFSSVGGWIATRAGGHYA
                     TGPTHIDDFVESVRMVSPAGLWQSRRLPASGAGPDPDRILLGSEGTLGVITEAWLRVQ
                     PRPSFRAGATVRFDSLTDAVSAVRTIAQSGLDPSNLRLLDRVEALNSLVADGTCDVLV
                     LGFESADHDVGPALARALECCRDHGGAAMGEAGSSGQGSSGSAAESWRGSFTRMPFYR
                     DVLMGYGIVSETFETAVTWDGFVGLYDAVLSSVGAALTAEGLSPASVSCRFTHVYPDG
                     VAPYFTVLAKGTPGDLDRQWWPIKQAAADAIESAGGTITHHHAVGRDHVPWYRAERPE
                     PFGAALRGLRSELDPAGVMNPGVLDW"
     gene            complement(4177..4557)
                     /locus_tag="PLK64_11725"
     CDS             complement(4177..4557)
                     /locus_tag="PLK64_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80462.1"
                     /translation="MTTSSLHEFAPRVSRTGPRETGEYEELIYCIIFWSHTGPDPVDA
                     WLSDGFYLYDVNAQDALNWGRANVADGEILEVQALSVAGDDVVAYTLFGNRPTKNPQI
                     EPYPPPSVPRNALESRVRPLALRS"
     gene            complement(4626..4913)
                     /locus_tag="PLK64_11730"
     CDS             complement(4626..4913)
                     /locus_tag="PLK64_11730"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HPV80463.1"
                     /translation="MNAPKRPPLKRARRKLTATEIARRYGINPRTVRRAIAEPRDDFE
                     ARARARQAQALALIESGLTYQQAADQMGITRGTAAGLVHRARARRREESAA"
     gene            complement(4910..5788)
                     /locus_tag="PLK64_11735"
     CDS             complement(4910..5788)
                     /locus_tag="PLK64_11735"
                     /inference="COORDINATES: protein motif:HMM:NF015076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HPV80464.1"
                     /translation="MTTVGEQLDFSSQFADAWLPYRPLAGSKTGPKRRMGRDQALQQS
                     YIETNVTHVLRSLVVVDHDGGLLDWVMKDFLPPSWVAARQGSHDGHLVFALRDKVPLT
                     DAARRPPVNLLARIEHGMNTVLGGDAAYAGQFTKNPHHHDHLTLWGPAEATYSLRELA
                     DALRDLKAMPPAGHPRRNVTTSDVGRNCALFDITRRWAYPRRGDHARPATWDEVVLEH
                     ALDRNTHLIAHEFTKGPLRDGEVAAIARSVSRWTWRNITRTLSQEQARRARRLGQLRR
                     ERGITALVALDPELDA"
BASE COUNT          878 a         2020 c         2334 g         1021 t
ORIGIN      
        1 tgagggcgtc gcgggtttgg gagcggatct gggcggtgtg gtcggcggct tggtcgcggg
       61 cggcttcgat ttggcgcagt tgggcgcgta gcgcgtcggc ttcgcgttgc gcggcggctt
      121 gttctgcggt gtgggcgcgt tcgcgttcga ccagttgcac ctgtgtgacc gctagacgca
      181 cctccaggtc ggtggcggtg tcggttgcct cgggtgcggg ctcggggtcg gtggggtggc
      241 cggggttgag gccggcggcg agtaggtcgg cgagggggat gcgccacacg ccgttgtcgt
      301 cgctgatggc gttggggaac gcgccggctg cgcggcggcg tttgatcgtg ttcagtgcca
      361 ccccgcaggc gcgagcggct cccttttgag tgaaccggtg gcccagttcg tcggaccggt
      421 tgctggtgag gtcgatcagg ggctcggtgg ggtggaccat ggccgaaacg gtagccagac
      481 cccacccacg gcccccggta ggcgcgccgc gtgttgagcg ttggcccccg ccccggaccc
      541 ccccttggct gggacccagg gggggtccgt gaccccaccc acggaccccg ggggtccggg
      601 gtgggtggac ccccggtgga cccctggggt ggctggggtt gttcagcggg ccggtggccc
      661 gctgccacga aggccctggc gggcctcgtg gggcatcgag ctgcgctcga ttggccacgg
      721 gcggctggcc gggccaggtc cttcacctcg ctgcgctcgg agcggaccgc ccagccgccc
      781 tggctgccgg tgtccttgcc gcgtgcggtg ttcccaccct tcgggcaggg acacgcggcc
      841 tcagccacct agcgaggtat gactatcgac ctcgctacgc tcgcccggta gtccctcgcc
      901 cggccggtgg ccggggggtc cgcctggtcg gcgtcctggg gggctcgctg ggtcgctcgc
      961 cttggggagg ttgctggtgg agcaggtggt gtcgccgccg ttgccgaagg gtgatggtgg
     1021 gcggggtacg tcggtgacgg tgcggctgac tgccgaagag cttgcggcgg tgaagcgggc
     1081 tgcggctgcg gcggggcggc ggcagcactc ccgctgggcg cgggagcagc tgctggccgc
     1141 caccgaactg tgggaccgcg atgagccgag gtccgtggcg gaggccgagg cgcggctgcg
     1201 ggaggcgttg cgcagcagtg gggtggcgtt ggcgcgctcg gagttggccc gcatcggcag
     1261 caacctgaac caggcggtac gcgcggccaa caccgcggcg ctgagcgccg ccaggacggg
     1321 tcaggcggtc gatgtggcgg ggttgactgc ggcggtcgag gccgcccgcg agcaactggc
     1381 gaggctggcc gacgacgggg aggtcttggc gtgatcgcga agatcacccg gggtgggtcg
     1441 gcgggcggcc tggccgccta cctgcacggg cccggggccg gcaccgagca cgccgtggag
     1501 gtcgacggcc agcgggtggc cggtgggatg gtggtggcgt ccaacgtgcc cggggtccag
     1561 gtgggggacc tcgacgggcg ccggtggggg cgttggctgg accgggccgc ggccagccgc
     1621 ccagagatca aaggcccggt ctggcattgc tcgctgaacc tggcccccgg tgatcgggcg
     1681 ttgaccagcc tggaatgggc cgacgctgcc agcgagttcg tggcacgtat gggcatcccc
     1741 gcgagtcacc cgtgggcggc ggtgcagcac gacgaccgcg gcatccacat cgcggtgtcc
     1801 cgcgtcgacg acaccggacg ggtgtggcac gcccgccacg accggcgcac cgcgcagaag
     1861 gcccggcaag ccgttgagac ccgctaccaa ctggccgccg cacccaccca gaagaccgac
     1921 tacggcgccg tactacccgc accgccagcg gtcggggtcc acgttgtggg cagcgacacc
     1981 tggcgggtcc gcggccacga cggccgcacc gtcaccgacc ggctcggccg cgacggccgc
     2041 tggcggctcg tcaccaacgg catgaggacc ctacgcatcg ccgtagcccg cgaccaagac
     2101 cccgacaccc tcgacctggc cgcgcgcatt gcccgccaac gcgccgcgtt ctcgacggtg
     2161 caccccccca accaaacaac gcccgacccc accccgcagc ggtcgccata ccggccacca
     2221 cccacgcggg accccggccg cggctccggc cgcggccgct gagccagccg cgccgacaca
     2281 cgcttcaggc cgctgttccg gtcttacctg gcctacgagc gccgcgacct cgtgtctccc
     2341 ctacgcccgc tgcgcgcccc ggctcgttac gtgctcgacc ttctgacggc gttggacgag
     2401 atagtgccgg cggtgttcca actaggggtt tcctgtgaag gggcacgcgc acgcagagag
     2461 aaactgctgc tgtagatcac cagttctcgc ttgctgccgc ctggtgcgtt ctcggcctgg
     2521 tgttggcgct cgccttcttc cgttggcagc cccgggactg aggtcaggcg cgggatcacc
     2581 aatccaagac tccgggattc atgacaccag cgggatccag ttcgctgcgc agaccccgca
     2641 gtgctgcgcc gaagggttcc ggtcgctcag cgcggtacca agggacgtgg tcgcggccga
     2701 ccgcgtgatg gtgggtgatc gttccgccgg cggattcgat cgcatcggct gctgcctgtt
     2761 tgatcggcca ccactgccga tcgagatctc ccggagtgcc cttggcgagg accgtgaagt
     2821 agggggcgac accgtcgggg tagacgtgcg tgaatcgaca ggacacgctc gcgggcgata
     2881 ggccctcggc tgtcaatgcg gcgccaaccg aactgaggac ggcgtcgtac agaccgacga
     2941 acccgtccca ggtcacggct gtctcgaagg tctcggagac gatgccgtac cccatcagga
     3001 cgtcgcggta gaacggcatc cgggtgaagc tgccccgcca ggattcagcg gccgaaccgg
     3061 atgatccctg accactgctg ccggcctcgc ccatcgctgc gccgccgtgg tcgcgacagc
     3121 actcgagggc cctggccagt gcgggtccga cgtcgtgatc ggccgactcg aatcccagca
     3181 ccagcacatc acaggtgccg tcggccacca aggagttcag cgcttcgacc cgatccagta
     3241 gacgcaggtt gctcgggtcc aaccctgact gcgcgatcgt ccgcaccgct gaaaccgcat
     3301 cggtcaacga gtcgaaccgg accgtggccc ctgctcggaa cgacgggcgc ggctgcaccc
     3361 gcagccacgc ctcggtgatc acaccgaggg ttccctcgga tccgagcaga atccgatccg
     3421 gatcgggtcc tgcgccggat gctggcaggc ggcgggactg ccacagcccg gcgggcgaca
     3481 ccatccggac cgactcgacg aagtcgtcga tgtgggtggg ccccgtcgca tagtggccac
     3541 ccgcgcgggt ggcgatccac ccgcccaccg acgagaactg gtagctctgc gggtagtgcc
     3601 gcagcgccag tccgtggggt cgcagttgat ctgcgaacgc cggccccagt acacctgcct
     3661 gcacgcgggc ggcccgagag acagggtcca cctcgagtac ctgatcgagt cgctgcaggt
     3721 ccaaagagat cgagccggtg tggtcgtctc cgatgtccgg agtcacgcca ccgaccaccg
     3781 acgacccgcc cccgtagggg atcacggcca cagaggtcgc cgaggcccac tcgaggattc
     3841 gttcgatgtc cgtctcgtct cgggggtagg cgaccagatc gggggtgtgg ggggccaggc
     3901 cacagaaggc tcgggcgagg tcggggaacg acttccccca ggcgtgcgcg agtcgatcag
     3961 cccgcgccgc ggtcacgacg gcgcccaggc tgtccggtac caccatccgt ggctcgcgca
     4021 gttcgatggt ggctgggtcc accggggtgc gcggcgcgtg gtccacgccg aaccggtgcc
     4081 gggcgacctg ggcgatgagt tcccgttcgg cagcgctcac gggatccggc ccctcggtgc
     4141 cccatgcagc gaagcggttc aggcgaggcg tgaaggtcat gaacgcaacg ctagagggcg
     4201 aacgcgactc tcaagggcgt tgcgcggtac cgaaggcggt gggtagggct cgatctgcgg
     4261 attttttgta ggtcggttgc cgaacagcgt gtacgccacg acgtcatcgc ccgcgacgct
     4321 caatgcctgc acctcgagga tttcgccatc tgcaacattc gcgcgacccc agttgagcgc
     4381 atcttgagca ttcacgtcgt aaaggtagaa accgtcgctc aaccaagcgt cgaccgggtc
     4441 gggtccggtg tgggaccaaa agatgatgca gtaaatcaat tcctcgtact cgcctgtttc
     4501 ccgggggccg gtacgtgaca ctcggggcgc gaactcatga agcgaactgg tcgtcatgcc
     4561 cctcacgata ggtggggcag tcttgggttg gttttctcat ccacgtcggt cggatgcgct
     4621 cccgctcacg cagcagactc ctcgcgtcgt cgggcacgtg ctcgatggac gaggccggct
     4681 gcggtgccgc gggtgatgcc catttggtcg gcggcttgct ggtaggtcag gcctgactcg
     4741 attagcgcga gggcttgggc ttggcgggcg cgggcgcggg cctcgaagtc gtcacggggt
     4801 tcggcgatcg cccgtcgaac ggtgcggggg ttgatgccgt agcggcgggc gatttcggtg
     4861 gcggtcagct ttcggcgggc tcgcttcagg ggaggtcgct tgggggcgtt catgcatcta
     4921 gctccgggtc gagcgcaacg agcgcagtga ttcctctttc gcggcgcagc tggccgagtc
     4981 ttcgtgcacg tcgcgcttgc tcttggctga gtgtgcgggt gatgttgcgc caggtccagc
     5041 gggagacgct gcgggcgatg gcggcgactt cgccgtctcg tagtgggcct ttggtgaact
     5101 cgtgggcgat gaggtgggtg ttgcggtcaa gggcgtgttc gaggacgact tcgtcccagg
     5161 tggcggggcg ggcgtggtcc ccgcgtcggg ggtaggccca gcggcgggtg atgtcgaaca
     5221 gggcgcagtt gcggccgacg tcgctggtgg tgacgttgcg ccgggggtgg ccggcggggg
     5281 gcatggcttt gaggtcgcgc agcgcgtcag cgagttccct gagcgagtag gtggcttcgg
     5341 ctggccccca cagggtcagg tggtcgtggt ggtgggggtt cttggtgaac tgcccggcgt
     5401 aggcggcgtc accgccgagg acggtgttca tgccgtgttc gatgcgggcc agcaggttca
     5461 ccgggggccg cctggcggcg tcggtcaggg ggaccttgtc ccgcagcgcg aacacgaggt
     5521 gcccgtcgtg gctgccctgg cgggcggcca cccacgaggg gggcaggaag tccttcatga
     5581 cccagtccag caggccgccg tcgtggtcga cgaccaccag ggagcgcagc acgtgggtca
     5641 cgttggtctc gatgtaggac tgctgcaggg cctggtcgcg gcccatgcgc cgcttcggcc
     5701 cggttttgct gccagccagg ggccggtagg gcagccaagc gtcggcaaac tggctgctga
     5761 agtccagttg ctccccgact gtcgtcatgc tcagcgaccc tgccagcgaa cactctgcgt
     5821 gacgtggatt gagcgtcggc gtgtttcgac tagggcctga gcctaatcag ggtatttccc
     5881 tatttccgtc actccccgtg acgggcgtgg gtcccgggcg atactccccg tgactagggc
     5941 gtggtgaccc ggcgtgagcg cccccccgtc ctcgaaagtg caccacccgc cgggaaacct
     6001 acgaactttg ctttagggac tcctttgacc cctccgcccg ctccacagca accacggcag
     6061 ggaagcccag gaaggcgggt aggagccccg atcacggaaa gagccatacg gaagtacccg
     6121 cagggggttc ggggcttgtg aggccgcgag agcggtttta gcggatggtg agggccagca
     6181 cggtgactac ggcggcggtg gtgagggcgg ttagggcggc gactgcggcc cagagtccgg
     6241 tgatggtggc gcg
//
LOCUS       DAOMZY010000214         6205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_11872_length_6205_cov_6.220000, whole genome shotgun sequence.
ACCESSION   DAOMZY010000214 DAOMZY010000000
VERSION     DAOMZY010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6205
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11872_length_6205_cov_6.220000"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..625)
                     /locus_tag="PLK64_11740"
     CDS             complement(38..625)
                     /locus_tag="PLK64_11740"
                     /inference="COORDINATES: protein motif:HMM:NF019826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HPV80465.1"
                     /translation="MRADRLVSLVLLLRQRGQLTADALAKELEVSPRTVLRDIEALSA
                     AGVPVYAERGRHGGYALLPGFRTELTGLNNDEALALLTAGSRPGEQIFGLGTALASAM
                     RKIVDALPEGHQVAATNAAQRFLVNPETDLLSRRLSQEELPDATLMVVRRAVLAGRKL
                     RIHYASRGQPPQWHTVDPIGLVTVRDKGYLLERVC"
     gene            757..1152
                     /locus_tag="PLK64_11745"
     CDS             757..1152
                     /locus_tag="PLK64_11745"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPV80466.1"
                     /translation="MERVAVNPVTWSVEMGFNQGEVITGHTRTLYCSGQTAMGADGTP
                     QHEGDMAAQVALSLDNLEAVLAAADMSLANLVRLNVYTTDVDLLFQHYGVLASRLGAA
                     QVAPTTTMLGVTRLAIPGQMIELEGTAVA"
     gene            complement(2511..4730)
                     /locus_tag="PLK64_11750"
     CDS             complement(2511..4730)
                     /locus_tag="PLK64_11750"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HPV80467.1"
                     /translation="MMSNAEAIEELLRLFTAWQADPSASFAHGLGKFKTAFQQLTAIR
                     RWGPEDRIRETGLREQWQENLDVVGQSFSTVMVEVELWYRRDATQRATAQAHVEHVIS
                     GSGGRVLDRSQIGEISYHALLAELPIQQVLSLLADGAESIRLLTTDEVMFVNPFTPMN
                     VAPPTTEPIRELRLPAGERTDGLPRVALLDGLPFNKHDALAGRLVVDDPDGLGENYPL
                     ASRHHGTAMASLIIHGDLSADGEPLDRPLYVRPIMRPHEFMARHEQIIPDRLLTDLLH
                     RAVKRIVEGEAGRGPVAPSVRIINLSIGAQARALVRRMSPVGRLLDWLAHSYNLLFVV
                     SAGNHLGPLTIPADAISNAESARSAAIRSAYDAALLRGILPPGDALNAVTIGATHSDG
                     FGDIPIPDTVWDITHQNGPAHYSASGPGVDRSVKPDLHHVGGRALYVRPVAPPDEQET
                     VAVELAPTAITGPGLQVAAPGRAGATNNTVFTVGTSNATALVAREASHLFDILEAGSG
                     DPDDEPLPDPQYHPLLVRALLAHASSWGEWEARLRAELGLTNQQARRHLTALLGYGRL
                     ETDRLGTAATNRAVLVGGGLIARDQRHTYELPLPPSLRARAEWHRFTITLAYMVPTVG
                     QLNRYRSAKVYFATPDMQLAGGDRIDAEHNSVRRGSLQHEIVQGTRTMVFGDDGTYPV
                     HVECMDDAQRLGAGKTVRYALVVSVETAEQTSTTIHDEVRSRLRQQVRDRARGRVQS"
     gene            5094..5510
                     /locus_tag="PLK64_11755"
     CDS             5094..5510
                     /locus_tag="PLK64_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80468.1"
                     /translation="MTSTYQTAFGQAQSAWDATSSPGYFNYEPDNGDPMVEVRDGSYS
                     WGAWAQTSWQGCPTGLWAYNEVYIAFNTRTMGGLTSSQKKLVAEHEAGHAYGLDHVSA
                     TCSSSSKAVMSQGETKFSCSGTAPWADDVNGVNAKY"
     gene            5521..6102
                     /locus_tag="PLK64_11760"
     CDS             5521..6102
                     /locus_tag="PLK64_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80469.1"
                     /translation="MNKRIPILVIASALALSACGSSTPTVSMDYPTYSTVAELDRVAD
                     VVVLGQIGARTSTEYDNGGNEEVGDEGQPLGIPMAFFDFTVQKVLQGDLTDKTITVEW
                     IDTSKVTLEDSTSDLKQGQTVVLWLQHLTTQDAPGVTSVSDFWVPVSGDNGVMDVAAG
                     HATARSETLTGLKTPGQERLSISQEALAAALAS"
BASE COUNT         1023 a         1850 c         2194 g         1138 t
ORIGIN      
        1 tcctcggaat ggtgatcctg acccaccgag ccaaccatta acagacacgc tctagtaagt
       61 agcccttgtc tcgcacggtg accaggccga tcgggtccac tgtgtgccat tggggtggct
      121 ggccgcgcga ggcgtagtgg atccgcagtt tgcgtccggc gaggaccgcg cgtcgcacca
      181 ccatcaacgt ggcgtcgggc agctcttcct ggctcaggcg acgggaaagt aggtcggtct
      241 cggggttgac gaggaaccgt tgtgccgcgt tggtcgcggc gacctggtgg ccttcgggga
      301 gggcgtcaac gatcttccgc attgctgagg cgagagccgt cccgagtccg aagatctgct
      361 cgcctggcct tgagccagcg gtgagcagcg cgagtgcttc gtcgttgttg aggcccgtga
      421 gctcggtccg gaacccaggt aggagtgcgt acccaccgtg ccggccacgt tcggcataga
      481 cggggacgcc ggccgcggaa agcgcttcga tgtcgcgcag gacggtgcgc ggggagacct
      541 cgagctcttt ggccagggca tccgcggtca actggccgcg ctgacgcagc agcaggacca
      601 aggacaccag ccggtcagct cgcacgacga agacgttatc cgaatacctg acacaaggtg
      661 tcgtgattca gcgtcagggt ggtcacgcgc cgccgaaccg cggcgacgcc cgaatgcggg
      721 caaccccgat ggaatctgat tggagctgat gtggcaatcg aacgagtggc cgtcaacccg
      781 gtgacgtggt ctgtggagat ggggttcaac cagggcgagg tgatcaccgg ccacacccgg
      841 acgttgtact gctctgggca gaccgcgatg ggagccgacg gaactcctca gcatgaggga
      901 gacatggcag cacaggttgc gttgagcctg gacaacctgg aggcggtgct ggccgcggcc
      961 gacatgtcac tggccaacct cgtgcggctc aacgtgtaca ccaccgacgt cgacctgctc
     1021 ttccagcact acggagtgct ggcgtcgcgg ctgggagcag cccaggtagc gccgaccacc
     1081 accatgctcg gcgtgacacg actggcgatc cccggccaga tgatcgagct ggaaggcacc
     1141 gcggtcgcct gagcgacccc tgctgcccgc tccggtcacc gtcggcgcgg gcagccaggc
     1201 agggaggggt gatcgctgat gcaccagagc cggtcgtctt ccatgaagca tccggtggca
     1261 cgtatggacg ggccatgtaa ggctgcgagg cgattgacgc cgcgaggctg gcggggtaac
     1321 cggcgtagcg gccgaggtct cgtttcgtcg gcccccattt gcgaggtgga gatggcagct
     1381 tgaggtctcg ccaatcgggt cgctccgcgg ggccgtgacg ttcttgagct tcaacggcgc
     1441 cgtcgaggtg cagcttcact tgtacgtcgt gcaggaacgt ctgggtcttg ttgacaacgg
     1501 tcacctcgtt cgcggccagg gcgtggacgg cgaacagttc gacagcttcc gcccatgcgg
     1561 cgcggaacct ttcctcccag gcatcgatct cggcgcggta ctcgtcctcg gagcgcttct
     1621 ccggctcttc cgtggcggca aggtggctcg cggccgatgt ctggtcggca aagaggcgcg
     1681 agagggaaga cgaggctccc atggccgcgc gaaagccgtc ggatgacccc gtcgcgaatc
     1741 ctgtggttgg ggactgcttg ggttccggtt tcgggagcgc agcgaggagg cgtcggcgcg
     1801 cgttggtgac gaggtcgtcg acggtctgct ggtcgactgt aaggggaacg acggtgccaa
     1861 cgaccgcgac gtcgagttcg acgggggccg gcgggcgtga ggctccgcgt gccatcagca
     1921 gatcgagttc atcggcgttt gcctcgcggg tctcaccgtc gccccggtag tagatgcgcc
     1981 cgctctggag tccttcgccg ttggatcggc agatgaacgg tggctgacct acctgcggtg
     2041 ggtcgacgag gatgaccaag acctgattcg tcgagccttc cacggggacg cgcacgacgt
     2101 cccagcgagg gccggccgcg ccgaggaacg gctgaatcac ctgggacagt gcgagcatct
     2161 cgatcggcgg tacaccttca atgccctgct tggtaatgcc gacgatcatc acgccgtagc
     2221 cctcgaaggc ctcagccgca cggtcgggca accggttcgc ggcgccgagg atgaacttcg
     2281 ccaccttggc cttgttcact ttcgtcgcga ggtcgggcgg ccccttcagt tcgaggtagt
     2341 gacgttccgc gagatcgcca gtgtctgcga cggcagtgac gagatgcgcg gcagccagct
     2401 cgccgcgcgg agcccgagcg aggtcgatgg cggcggcccg gtcttcattc acgctcacgc
     2461 ggcctcgatt ccgcgtggtg cgggccctga gcagcctgtt gtcgtgcaca tcagctctgc
     2521 acccgaccgc gtgcccgatc acgaacctgc tggcgcagcc tcgaccgcac ctcgtcatgg
     2581 attgtggtcg atgtctgctc cgcagtctcc acggacacca cgagcgcgta tcgcacggtc
     2641 ttgccggcgc cgagccgttg ggcatcgtcc atgcactcga cgtggaccgg gtaggtgcca
     2701 tcgtcgccga acaccatcgt gcgagtgccc tgaacgatct cgtgttggag gcttccgcgt
     2761 ctgaccgagt tgtgttcggc atcgatccgg tcgccgccag cgagttgcat gtctggcgtg
     2821 gcgaagtaga ccttggcgct ccggtagcgg ttgagctggc ccacagtggg gaccatgtag
     2881 gcgagggtga tggtgaagcg gtgccactct gcgcgtgcgc gaagcgaagg cggcagcggc
     2941 agctcgtagg tgtgacgctg gtcgcgtgcg atcaggcctc cgccgaccag aactgccctg
     3001 ttcgtggccg cggttccgag ccggtcggtt tcgagccggc catagcccag gagcgcggtc
     3061 aagtggcgac gggcttgctg gttggtgagg ccgagttcgg cgcgtagccg cgcctcccat
     3121 tcgccccagc tgctggcgtg ggcgaggagg gctctcacta gtagcgggtg gtactgcggg
     3181 tccggtaggg gctcgtcatc aggatcgccg gagccggctt cgaggatgtc gaacaggtgg
     3241 ctggcttcgc gggccaccaa cgcggtggcg ttgctcgtgc cgacggtgaa gacggtgttg
     3301 ttggttgcgc ccgctcgtcc gggcgcggcc acctgtaggc ctggaccagt gatggcggtc
     3361 ggagccagtt ccaccgcgac agtttcctgt tcgtcgggcg gggcgacagg gcgcacgtag
     3421 agcgctctcc cgccaacgtg atgcaggtcg ggcttgaccg agcggtcgac gccggggccg
     3481 ctcgcgctgt agtgagcggg gccgttttgg tgggtgatgt cccagacggt gtcgggtatc
     3541 ggtatgtcgc cgaatccatc gctgtgggtg gcgccgatgg tcacagcgtt gagggcgtcg
     3601 cctggaggga gaattcctcg cagcagtgcc gcgtcgtagg cggaccggat cgcggcggat
     3661 cgtgccgact cggcgttact gatggcatcg gctgggatcg tgagcgggcc gaggtggtta
     3721 ccggcgctga cgacgaacaa caggttgtac gagtgggcca accagtcgag tagccgtccg
     3781 acggggctca tccggcgcac aagcgctctg gcttgcgcac cgatggaaag gttgatgatg
     3841 cgcacgctcg gggccaccgg gcctcgaccg gcttcccctt cgacgattcg cttgaccgcg
     3901 cggtgcagca gatcggtgag tagccgatcc gggatgatct gctcgtgtct tgccatgaac
     3961 tcgtggggtc gcatgattgg gcggacatac agcggacgat cgagcggttc accgtcggcc
     4021 gagaggtcgc cgtggatgat cagtgaagcc atagcagtgc cgtggtggcg cgaggccagc
     4081 gggtagttct cgccgagccc gtcagggtcg tcgactacca gccggccggc aagggcgtcg
     4141 tgcttattga acggcaaccc gtccagcagg gctaccctcg gcaagccgtc agtgcgctcc
     4201 ccggcaggca agcgcagctc cctgatcggt tctgtggtcg gaggggcgac gttcatgggg
     4261 gtgaacgggt taacgaacat gacttcatcg gtggtcagca gccggatcga ctcggccccg
     4321 tcggccaaca gcgagagaac ctgttggatc ggcagctccg cgagcagggc gtggtagctg
     4381 atctcgccga tctgggagcg gtcgagcaca cggccaccac tcccggagat gacgtgctcg
     4441 acgtgcgcct gtgccgtggc gcgctgtgtg gcgtcgcggc ggtaccaaag ctcgacttcg
     4501 accatcacag tgctgaagga ctgaccgacg acgtcgaggt tttcctgcca ctgctcgcgc
     4561 aggccggttt cgcggatgcg atcctctggt ccccaccggc ggatcgcggt gagctgttgg
     4621 aaagcggtct tgaacttgcc caagccgtgg gcgaacgacg ccgacgggtc tgcttgccaa
     4681 gcggtgaaca ggcgcaggag ttcctcgatc gcctcggcgt tggacatcat taggtacagg
     4741 gagtgctgga cgggcttgtc cgtatgagag tgtcaagttc acttggcccc aggtgacggc
     4801 ttgacttggc cccagtcgat gacgcgtggg tcggtaccgc acggcgagtc ttgtcaggcc
     4861 aggtcggccg ccactacctt cttgtctgag ggtcttctca gtcgacacag ggggttcgca
     4921 tgcatgttcg gcgttttgcg gtttccgcat cgcagcggtc ccgggtcgct cttggcgcgg
     4981 tagcactgtt catggccgct ctggtggtga gcgcgtcgcc agcgtcggcg tacgccttcg
     5041 agtgcggaaa gttccttggt tctagcccca ccatcagcta ccggtactaa agcatgacga
     5101 gtacctacca gaccgcgttc ggtcaggcgc agtcagcgtg ggatgcgact tcgtcgcctg
     5161 gctatttcaa ctatgaaccg gacaacggcg acccgatggt ggaagttcgg gacggttctt
     5221 acagttgggg cgcctgggct cagacatctt ggcaggggtg cccgacgggg ctgtgggctt
     5281 acaacgaggt gtacatcgcc ttcaacaccc ggactatggg agggctgacc tcatcccaga
     5341 agaagctcgt tgctgaacat gaggccggac acgcatatgg actcgatcac gtcagcgcca
     5401 catgcagttc cagcagcaag gcagtgatga gccaggggga gaccaagttc tcgtgttcgg
     5461 gaaccgcgcc ctgggccgat gacgtcaatg gcgtgaacgc caagtactga ggaggcgagg
     5521 gtgaacaagc ggattccgat tctcgtgatt gcatcggcgc tggccctgtc cgcatgcggc
     5581 agttccacgc ccactgtctc tatggactac cccacgtaca gcacggtggc agagctcgac
     5641 cgcgttgcgg acgttgttgt tcttggccag atcggcgccc ggacgtctac ggagtacgac
     5701 aacggaggca acgaggaggt cggggacgag ggacagccgt tgggcatacc gatggcgttc
     5761 ttcgacttca ctgtccaaaa ggtcctgcag ggagacctga cggacaagac cattacggtc
     5821 gaatggatcg acacctccaa ggtcacgctg gaagattcca cctctgacct gaagcagggc
     5881 cagaccgtag ttctgtggct gcagcacctg acgacgcagg atgcgccggg agtcacgtcg
     5941 gtctcggact tttgggtgcc ggtctccggc gacaacggag tgatggatgt cgcggcgggc
     6001 cacgcgaccg cccgcagcga gactttgacc ggtttaaaga ccccgggcca ggagaggctg
     6061 agcatcagcc aggaggcgct cgccgctgcc ttggcgtcgt gaaggggagc ggtcagccgg
     6121 ggtgacccgc aagccgtgat gcctgtcgcg cgtgggcgag gcggtaggag tgggtgccgg
     6181 tttcgatgat ggtgccgtgg taggt
//
LOCUS       DAOMZY010000215         6186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_11921_length_6186_cov_6.120861, whole genome shotgun sequence.
ACCESSION   DAOMZY010000215 DAOMZY010000000
VERSION     DAOMZY010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6186
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11921_length_6186_cov_6.120861"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..911
                     /gene="tsf"
                     /locus_tag="PLK64_11765"
     CDS             81..911
                     /gene="tsf"
                     /locus_tag="PLK64_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPV80470.1"
                     /translation="MANFTAADIKALREQTAAGMMDVKRALEEADGDMAQAAEILRIK
                     GLKGVTKREGRSASNGLVAAKVENGVGTLVEVNCETDFVAKGERFIALADQVLAQAVA
                     TEAADAETLLASTLPDGRTVAELLVEGNAATGEKIEVRRVARVTGDRVVSYLHKTNRD
                     LPAQIGVLVATTGGDEEIAHDVAMHIAAFSPTVLGREDVPEDVVANERRIAEATAREE
                     GKPEAALPRIIEGRVNGFFKENVLLEQAFAKDQKKSVAQVLKEAGAEASSFARFRVGN
                     "
     gene            1146..1901
                     /gene="pyrH"
                     /locus_tag="PLK64_11770"
     CDS             1146..1901
                     /gene="pyrH"
                     /locus_tag="PLK64_11770"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPV80471.1"
                     /translation="MSAPDTVAPAPDESHPAYRRVLLKLSGEVFGGGRTGVDPDVVKE
                     IAGQIAAAVTDSVQVAIVVGGGNFFRGAELQQKGMDRARADYMGMLGTVMNCLALQDF
                     LEKAGVPTRVQTAITMGQVAEAYIPLRAIRHMEKGRVVIFGAGAGMPYFSTDTVSAQR
                     ALETHCDRLLIAKSGVDGIYTADPKIDPTATKIEALTFADALGRGLKILDAAAFALCA
                     ENRLPMLVFGMEGEGNITRALRGERIGTLVTAG"
     gene            1930..2487
                     /gene="frr"
                     /locus_tag="PLK64_11775"
     CDS             1930..2487
                     /gene="frr"
                     /locus_tag="PLK64_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018771654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPV80472.1"
                     /translation="MIDETLFEAEEKMEKAVEVAKEDFAGIRTGRANPGMFNKLLVDY
                     YGSPTPMQQLASFQTPEARTIIIKPFDAKAMHGIERAIRDSDLGVNPSNDGVVIRCIL
                     PVLTEERRRDYIKLAHHKAEEARVSIRNVRRKAKTDLEHLIKDGEVGEDDGNRAEKEL
                     DQMTKKYVDTIDHLVKGKEAELLEV"
     gene            2528..3370
                     /locus_tag="PLK64_11780"
     CDS             2528..3370
                     /locus_tag="PLK64_11780"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPV80473.1"
                     /translation="MSEAPLASPSSRAGRNLPAAIGVGLLLGALIIGTHVWRKELFLV
                     LATASVSVGCWEIAKAMQRKGIRAPVFPVVLGCVVQMTGAYFLGEQALFVAFVLTAAL
                     IVMWRGSESMSGAATDVSAGVFIAAYPALLAGFAMLLLAAPDGAWRSFTFLCVTVASD
                     VGGYAVGVLAGRHPMAPRISPKKTWEGFAGSFLGCILVGIICVTVGLHGPWWVGAILG
                     VVTAPAATLGDLVESAIKRDLGIKDMSNILPGHGGLMDRLDSLMVSAPFIWATLTILL
                     PLPK"
     gene            3491..4582
                     /gene="rlmN"
                     /locus_tag="PLK64_11785"
     CDS             3491..4582
                     /gene="rlmN"
                     /locus_tag="PLK64_11785"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPV80474.1"
                     /translation="MPPRHLADLDAAGRKAAVEALGHKGFRAGQLSKHYFERLVDAPE
                     EMTDLPGAARTELVDALLPRLLTPVKHLVADEGRTVKSVWRLHDGALVESVLMRYPER
                     VTMCISSQAGCGMNCPFCATGQGGLTRNLSTAEIVEQVVAGARMLRRGEVPGGDADAE
                     RERPLRVSNVVFMGMGEALANYSRAIAAIRRLTDPAPDGLGMSARGLTMSTVGLVPAI
                     DRLAGEGIPVTLALSLHAPDDELRDELVPINTRWRVDEALDAAYRYFEATGRRVSIEY
                     ALIRDINDQSWRADLLGEKLARRGRGWVHVNPIPLNPTPGSKWTASRTGVQQQFVERL
                     RAHGIPTTVRDTRGREIDGACGQLAATTA"
     gene            4726..5772
                     /gene="tdh"
                     /locus_tag="PLK64_11790"
     CDS             4726..5772
                     /gene="tdh"
                     /locus_tag="PLK64_11790"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HPV80475.1"
                     /translation="MRALTKVNAGPGLELVDRPEPTCGPLDVKVRVLRAGLCGTDLHL
                     EQWDDWAASTVKAPMVIGHEFYGEIVEVGEDVPAGTAVAVGARVSGEGHIVCGHCRNC
                     RAGRRHVCINTIGIGVNRDGAFADFIVLPATNVWVQPDAIDPDLGAIFDPLGNATHTA
                     LQWPLVGEDVLITGAGPIGVIAAAIARHAGAKNVVVTDISDYRLGLAKAAGADLTVNV
                     ATTRIKDAQRELGMREGFDIGLEMSGAPSAVAEMLANLNHGAKVAMLGLPKDPYPIDW
                     GRVITHMITLKGIYGREMFETWYLMSSMLATSETFTSAVSSVITHRFPASQWADAFAA
                     ARSGECGKVVLNWS"
     gene            5895..>6186
                     /locus_tag="PLK64_11795"
     CDS             5895..>6186
                     /locus_tag="PLK64_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HPV80476.1"
                     /translation="MSELTFATADLTTFARVDELGLEVVGQRVEPGRAVLACRVVEPD
                     DWCRRCGCQGRVRDTVRRRLAHEPFGWRPTMLLVTVRRYRCPGCGHVWRQDTS"
BASE COUNT         1004 a         2155 c         2092 g          935 t
ORIGIN      
        1 gtcgaggccg ctgccgacga gccgcaggcc tgagccgcgc ggcataccca ttcctgaccg
       61 aaaccgcaga gagagtaacg atggcgaatt tcaccgccgc tgacatcaag gcgctgcgcg
      121 agcagaccgc cgccggaatg atggacgtca agagggccct cgaagaggcc gacggcgaca
      181 tggcccaggc cgcagagatc ctgcggatca agggcctcaa gggtgtgacc aagcgtgagg
      241 gccgatcggc ctccaacggt ttggtcgccg ccaaggtcga gaacggagtt ggcaccctcg
      301 ttgaggtcaa ctgcgagacc gacttcgtcg ccaagggcga gcgcttcatc gcgctggctg
      361 accaggtgct ggcccaggcc gtcgccacgg aggccgccga cgccgagacg ctgctggcga
      421 gcacgctgcc cgacggacgg accgtcgccg agttgctcgt cgagggcaac gcggccaccg
      481 gcgagaagat cgaggtccgt cgcgtggccc gggtgaccgg cgaccgcgtc gtgtcctacc
      541 tgcacaagac taaccgcgac ctgcccgcgc agatcggtgt tctcgtggcc accaccggtg
      601 gcgatgagga gatcgctcac gacgtcgcga tgcacatcgc ggccttctcc ccgacggtgc
      661 tcggtcgtga ggacgtcccg gaggacgtcg tcgccaacga gcgtcgcatc gccgaggcca
      721 cggcccgcga ggagggcaag cccgaggctg ccctgccgcg gatcatcgag ggccgggtca
      781 acggcttctt caaggagaac gtcctgctcg agcaggcctt cgccaaggac cagaagaagt
      841 cggtcgccca ggtgctcaag gaggccggcg ccgaggcgtc atccttcgcc cgcttccgcg
      901 tcggcaactg atcgcgtcag cgcagacacc cgcaccatcc ggtatgccgc ccgcccccgt
      961 tcgggggccg ggcggcatac cgcgttgagg gccgcagttt ccctgggggc ggctcccgtc
     1021 gcaccccgct caaccccctc gctcgcaacc ccctcgcacc acccccgccc gactgctgga
     1081 cgccgggtgc ggccggggat gtggcaagat cgaacgaccg cccaagccac aatgaaggag
     1141 ccgccatgag cgccccggac accgttgcgc ccgccccgga cgagagccac ccggcatacc
     1201 ggagggtctt gctcaagttg tcgggtgagg tcttcggcgg cggccgcacg ggcgtggacc
     1261 ccgacgtcgt caaggagatc gccggccaga tcgcggcggc cgtgaccgac agcgtccagg
     1321 tggccatcgt cgtcggcggg ggcaacttct tccgcggcgc ggagttgcag cagaagggca
     1381 tggaccgggc tcgcgccgac tacatgggca tgctgggcac ggtgatgaac tgccttgcgc
     1441 tccaggactt ccttgagaag gccggggtcc cgacgcgggt gcagaccgcg atcaccatgg
     1501 gccaggtcgc cgaggcctac atcccgctgc gtgccatccg gcacatggag aagggccgcg
     1561 tcgtcatctt cggcgcgggc gccggtatgc cgtacttctc caccgacacg gtcagcgccc
     1621 agcgcgcgtt ggagacacat tgcgaccggc tgctcatcgc caagagcggg gttgatggca
     1681 tctataccgc cgaccccaag atcgacccga ccgcgaccaa gatcgaggcg ctgaccttcg
     1741 ccgatgcgct ggggcgcggg ttgaagatcc tcgacgctgc agccttcgcg ctgtgtgccg
     1801 agaaccgcct gccgatgctc gtgttcggca tggagggcga gggaaacatc acccgtgccc
     1861 tgcgaggtga gaggatcggc acactggtga ccgccggatg acccacatgc aaggagcagg
     1921 acgaccgcga tgatcgacga gacactcttc gaggccgaag aaaagatgga aaaggccgtc
     1981 gaggttgcca aggaagactt cgccggcatc cgcaccggac gggccaaccc ggggatgttc
     2041 aacaagctcc tcgttgacta ctacgggtcg ccgacgccga tgcagcagtt ggcgtccttc
     2101 cagacccctg aggcgcggac aatcatcatc aagccgttcg acgcgaaggc gatgcacggc
     2161 atcgagcgcg cgatccgcga ctccgacctc ggggtcaacc ccagcaacga cggcgtcgtc
     2221 atccggtgca tcctcccggt cctcaccgag gagcgccgcc gcgactacat caagctcgcc
     2281 caccacaagg ccgaggaagc ccgggtgtca atccgcaacg tccggcgcaa ggccaagacc
     2341 gacctcgagc acctcatcaa ggacggcgag gtcggcgagg acgacggcaa ccgcgccgag
     2401 aaggaactcg accagatgac caagaagtac gtcgacacga tcgaccacct ggtcaagggc
     2461 aaggaggccg agctgctcga ggtgtgaagc ctcgaccctc ggcgtcccga tgagcgcccc
     2521 tcagcccatg tccgaggccc ccttggcctc cccgtcctca cgtgccgggc gcaacctccc
     2581 ggccgcgatc ggtgtcggac tgttgctggg tgccctcatc atcggcaccc acgtctggcg
     2641 taaggaactc ttcctcgtcc tcgcaacggc atcggtctcc gtcggctgct gggagatcgc
     2701 caaagcgatg cagcgcaagg ggatccgggc gcccgtcttc cccgtggtcc tcggctgcgt
     2761 cgttcagatg acgggtgcct acttcctcgg cgagcaggcg ctgtttgtcg ccttcgtgct
     2821 caccgcggcc ctgatcgtca tgtggcgcgg cagcgagagc atgagcggcg cggcgaccga
     2881 cgtcagcgcg ggcgtgttca tcgcggcata ccccgcgttg ctggcgggct tcgcgatgct
     2941 cctgctcgcc gctccggatg gcgcgtggcg tagcttcacc ttcctgtgcg tgaccgtcgc
     3001 gagcgacgtc ggagggtatg ccgtcggcgt cctcgccggg cgccacccga tggcgccgcg
     3061 gatcagcccg aagaagacct gggaaggctt cgccggctcg ttcctcggct gcatcctcgt
     3121 cggcatcatc tgcgtcaccg tcggcctgca cgggccgtgg tgggtcggcg cgatcctcgg
     3181 ggttgtcacc gcgcccgccg ccacgctggg cgacctggtc gagtcggcga tcaagcgtga
     3241 cctcgggatc aaggacatgt ccaacatcct gcccggccac ggcggcctga tggaccggct
     3301 cgactcgctg atggtctcgg cgccgttcat ctgggccacg ctgaccatcc tccttccgct
     3361 gcccaagtga ctcggtgacc cggtgaccaa cccgcccgaa gcgaccggcg ttccttccgc
     3421 cgcgcccgca ccccgtgcca cgcggcggcc cgcccccggc gagctcacct tcgaagcccc
     3481 ccggcgcggt atgccgccgc gtcacctcgc tgacctcgac gcagccgggc gcaaggcagc
     3541 cgtcgaagcc ttgggccaca agggattccg ggccggccaa ctgtccaagc actacttcga
     3601 gcggctcgtc gacgcgccgg aggagatgac cgacctgccc ggcgccgccc gcactgagct
     3661 cgtcgacgcc ctcctgcctc gtctgctcac cccggtcaag cacctcgtcg cggacgaagg
     3721 ccgcaccgtt aagtcggtgt ggcgactgca tgacggcgcc ctcgtcgaat cggtactcat
     3781 gcgttacccc gagcgggtga cgatgtgcat ctccagccag gccggctgcg ggatgaactg
     3841 ccccttctgt gcgaccggtc agggcgggct gacccgcaac ctgtcgacgg ccgagatcgt
     3901 cgagcaggtc gtcgccggag ccaggatgct gcgtcgaggt gaagtccccg gaggcgacgc
     3961 cgacgccgag cgcgagcgtc ccctgcgggt gtcgaacgtc gtcttcatgg ggatggggga
     4021 ggccctggcc aactactccc gtgccatcgc cgcgatccgc cggctcaccg acccggcgcc
     4081 cgatgggctg gggatgtcgg ctcgcgggct gaccatgtcg acggtcgggc tcgtgccggc
     4141 catcgaccga ctggccggcg agggcatccc cgtcaccttg gcgctgtcac tgcatgcccc
     4201 cgacgacgag ttgcgcgacg aactcgtgcc catcaacacg cggtggcggg tcgatgaggc
     4261 gctggacgcg gcataccgct atttcgaggc gaccggccga cgcgtctcca tcgaatacgc
     4321 cctcatccgc gacatcaacg accaatcctg gcgcgctgac ctgttggggg agaagctcgc
     4381 ccgccgcggt cgcgggtggg tgcacgtcaa ccccatcccg ctcaacccca cgcccggctc
     4441 aaaatggacg gcgtcgcgta ccggagtcca gcagcagttc gtcgagcggc tgcgggcaca
     4501 cggcataccg acgacggtcc gcgacacccg cgggcgcgag atcgacggtg cctgtggcca
     4561 gctcgcggcg acgaccgcct gacccgggct gatcctccga acgtcccaat gagtgggctc
     4621 ggcccccgct agattcggta tgccgcccgc tcacccccgc gggccgcctc cggtccgcca
     4681 gggcactcgc gagccggcgt cacggcatac gggaaggatc cccacatgcg cgccctgacc
     4741 aaggtgaacg ccggacccgg cttggaactg gtcgaccgac ccgagccgac ctgcggcccg
     4801 ctcgatgtca aggtgcgcgt gctgcgcgcc ggcctgtgcg gcaccgacct gcacctggaa
     4861 cagtgggacg actgggccgc ctcgacggtc aaggcaccga tggtcatcgg gcacgagttc
     4921 tacggcgaga tcgtcgaggt cggggaggac gtgccggcgg ggacggccgt cgccgtcggc
     4981 gcgcgggtgt cgggcgaggg gcacatcgtc tgcggccact gccgcaactg ccgggccggc
     5041 cgccgccacg tgtgcatcaa caccatcggc atcggcgtca accgggacgg agcgttcgcc
     5101 gacttcatcg tcctgcccgc gacgaatgtc tgggtccagc cggacgccat cgaccccgac
     5161 ctgggagcga tcttcgaccc gctcggcaac gcgacccata cggcgctgca atggccgctt
     5221 gtgggcgagg acgtcctcat caccggggcc gggccgatcg gggtgatcgc cgcggccatc
     5281 gcccggcacg cgggagccaa gaacgtcgtc gtcacggaca tctccgacta tcggctgggg
     5341 ctggccaaag ccgccggtgc ggatctcacg gtcaatgtgg ccacgacccg gatcaaggac
     5401 gcgcagcgcg agttgggcat gcgggaaggc ttcgacatcg gcctggagat gtccggtgcc
     5461 ccgtcggccg tggccgagat gctcgccaac ctcaaccatg gcgccaaggt cgcgatgctc
     5521 ggcctgccga aggaccccta cccgatcgac tggggtcggg tgatcaccca catgatcacc
     5581 ctcaagggca tctacggccg cgagatgttc gagacctggt acctcatgtc gtcgatgctc
     5641 gccacctccg agaccttcac gtcggcggtg tcgtcggtca tcacccatcg attcccagcc
     5701 agccagtggg ccgacgcctt cgcagcggcc cgctcaggcg agtgcggcaa ggtcgtcctt
     5761 aactggagtt aatcggctct acccaatcga gagtgtgggt gaggtcccgg cgggtcggcg
     5821 cgtcgctgac ggggtgaggg tcgaggtctt ccgatgattg aggttcctac gccttcgatc
     5881 tggaagaccc cgacgtgtct gaacttacct tcgcgactgc tgatctgacg acgttcgccc
     5941 gcgtggacga gcttggcctc gaggtcgtgg ggcaacgtgt tgagccaggt cgggcggtgc
     6001 tggcgtgtcg ggtcgtcgag ccggatgact ggtgtcggcg gtgcggctgc cagggccggg
     6061 tacgagacac ggtgcgtcgg cgcctggcgc acgagccgtt cgggtggcga ccgacgatgc
     6121 tgttggtgac ggtgcgccgg taccggtgcc cagggtgtgg gcatgtgtgg cgtcaggaca
     6181 ccagta
//
LOCUS       DAOMZY010000216         6166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_11974_length_6166_cov_4.388480, whole genome shotgun sequence.
ACCESSION   DAOMZY010000216 DAOMZY010000000
VERSION     DAOMZY010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6166
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11974_length_6166_cov_4.388480"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PLK64_11800"
     CDS             complement(<1..609)
                     /locus_tag="PLK64_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HPV80477.1"
                     /translation="MLDQLWQVIALVVVIAVIGVGLVVGLLRGRRGKAPSLPPTTRPS
                     DQPGVGRPGVGSPDGGGVATKPGPPVTTSPAVEAPELPALAEPETPAYERPESARGRL
                     ARLRARLARSNSAIGKGLLALLSRGGLDEAAWEDVEDTLLTADLGGEATMELVESLKT
                     KVKVEGTTDESVVRGWLREDLLRLVDPTMDRRVASSRVGDRPA"
     gene            complement(694..1389)
                     /locus_tag="PLK64_11805"
     CDS             complement(694..1389)
                     /locus_tag="PLK64_11805"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPV80478.1"
                     /translation="MFVTADDGVVLHAASGGEGPDVVVLSGGPGCVHYLADESIAPAG
                     FRCWFPDPRGVGRSGGGPHSMALAVADLEALRRAIGAEQWIVLGHSWGSDLAVRYALD
                     HPDHVRGVVGIAGHGLHRDQEWSAAYEAGNARQIPMPIEWVPEVYSALRASFTEWIHE
                     PELWRQLADSPVPMTFIAPGDDFRPTWPLQQLAALVPRGAFEVVPGTGHDFWSTDPHL
                     WRAVCTAACSALK"
     gene            complement(1460..2176)
                     /locus_tag="PLK64_11810"
     CDS             complement(1460..2176)
                     /locus_tag="PLK64_11810"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HPV80479.1"
                     /translation="MRANAAPVLRVRDARVAIAWWARLGFQVEFEHRFEPHLPLYVGL
                     RRDDAIVHLSEHAGDARAPGLVYVYVPDIDRIAAEFGVSVNEAPWAREVQLVDPDHNR
                     VRCGCVPPASGASPRMRVSTVNLSAADVPGLSRFYAQLLGYAVDEDNPDWHYLAASDG
                     SVALSVQREPTHQPPVWPQTAEAQQMQAHLEVRVDGDLGAAVDRAIALGATLAAYQPQ
                     DDVRVLFDPAGHPFCLWVPS"
     assembly_gap    2254..2353
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2354..3939)
                     /locus_tag="PLK64_11815"
     CDS             complement(<2354..3939)
                     /locus_tag="PLK64_11815"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HPV80480.1"
                     /translation="MTSGIIRANLDLSIRPQDDLFGYTNGTWFAQTEIPADRGRYGTF
                     DHLREQAEHDLREILDEAVASGAAPDTSMGRVALLYGAFLDEAAVQAAGLGAVAGLLA
                     EVDGVTNADDLVALLARLDRIGIGGAFHHYVNTDDRASDRYIGYLHQGGLGLPDESYY
                     REESYAETRAAYLRHVTAMLTLAGRPEPEAAAVRVLSLETRLAAGHLDKVADRDPVAT
                     YNLFTAAALADLAPHVDWAGYAAALGSPNALAEVVVRQPDYLRALSDALAEVSIGTWQ
                     DWLAWQILHSRAPFLPQEFVEENFDFYGRTLAGTPQLRERWKRGVELIDRTLGEALGE
                     LYVARHFPPAAKARMSELVSNLVEAFRRSFADRQWMGEDTKAQALAKLEQFTPKIGYP
                     ETWRDYSALVLTGDLLADVAAANAFESDRSLAKLGRPVDRGEWFMSPQTVNAYYNPGM
                     NEIVFPAAILQPPFFDVEADDAVNYGGIGGVIGHEVGHGFDDQGSQYDGSGNLRNWWT
                     DDDREAFDALGAKLVEQFNRL"
     gene            complement(4049..5668)
                     /locus_tag="PLK64_11820"
     CDS             complement(4049..5668)
                     /locus_tag="PLK64_11820"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HPV80481.1"
                     /translation="MSQDHGTEGRGRVLVIGNGPVGQTAALCLARWGIPVLVLDSRPE
                     RDPVGSKALCQQRDVLDLWDACGAGAVATEGLTWSRARTFYKDKELFCWEIPDPGRSP
                     LPPFVNISQARSEAILDEAIAAQPLIEVWWGTEVVGVAGGKSDSDGLATVVCRSGFGE
                     FEVAGEYAVVCAGSKGGPLREALGVTFPGRTFDDSFLICDIETTLKGWENERRFYFDP
                     EWNPGRQVLIHPCPNSIFRIDWQVDTDFDLAAAQADGSLDKRIRKVIGKKRDYRIDWV
                     SVYRFHARSADRFRVGRFLLAGDVAHLVAPFGARGLNSGVPDVENAAWKIAFAMKGWA
                     GPGLVGSYESERQAAARENLAVTAATMDFLVPQTKKAKHRRKEILKRARKDKKVIPLV
                     DSGRLAEAFWYVDSPLTTPDPSSFWPGRPPRGQAPTPVPGVILPDAVTVSAGHGKSRV
                     RELAREGLLILVGEAVNTHEVEGLLSGVVPAGLPVRVLHFSDLGDEDVVATGLQAVAE
                     ELWLIRPDAHTAACVRTPEALADAVHTLLGH"
     gene            complement(5720..>6166)
                     /locus_tag="PLK64_11825"
     CDS             complement(5720..>6166)
                     /locus_tag="PLK64_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPV80482.1"
                     /translation="AWLPKHKIMFAGDLVEAKAALYTGDAFHQEWASTTLDAVKAMGA
                     EAIVGGRGEIPRGEAAVNAAIEQSRGFITSIIDNVRATQAAGGTLKQAFDACHAALSP
                     TYGHWPIFEHTLPFDVARTWEELQGVERPTIWTAERDQEIWAQLQG"
BASE COUNT          993 a         2127 c         2058 g          888 t
ORIGIN      
        1 cgcaggccgg tcccccaccc gcgacgaggc gacgcggcgg tccatcgtcg ggtcgacgag
       61 gcgcaggagg tcttcgcgca gccagccccg caccaccgat tcgtcggtgg tgccctcgac
      121 cttgaccttg gtcttgagcg actcgacgag ctccatcgtg gcctcgcccc cgaggtctgc
      181 ggtcagcagc gtgtcctcga cgtcctccca cgcggcctcg tcgagaccgc cgcgggacag
      241 cagggcgagc agccccttgc cgatggccga gttgctgcga gcaagccgag cgcgcaaccg
      301 cgcgagccgc ccacgagcgc tttccggcct ctcgtatgcc ggtgtctccg gttcggccag
      361 cgccggcagc tccggtgcct cgacggcggg agaggtcgtg accggcgggc cgggcttcgt
      421 ggcgacgccg ccgccatcgg ggctgccgac ccccgggcgg ccgacgcccg gctgatcgct
      481 gggccgcgtc gtgggcggga gcgagggcgc cttgccgcgc cggccacgga gcagcccgac
      541 gaccaggcca acgccgatga cggcgatgac gacaacaaga gcgatgacct gccagagctg
      601 atcgagcatg gtcacatcct cacatgcgcc gggcgaggcc cgtcagttcg gcacgtccgc
      661 tgtcgacggt gcggcgagcc gaaccgagtc cgcctacttc aaggccgaac aagcggccgt
      721 gcacacggcg cgccacaggt gggggtccgt ggaccagaaa tcatgacccg tgcctggcac
      781 cacctcgaag gcgccccggg gaaccaacgc cgccaattgc tgcagcggcc acgtcggtcg
      841 gaagtcatcc cctggcgcga taaaggtcat cggcaccggg ctgtcggcca gctgccgcca
      901 caactcaggc tcgtggatcc attccgtgaa cgacgcccgg agggcggaat acacctctgg
      961 cacccactcg atcggcatgg ggatttgacg cgcatttccc gcctcgtagg ccgctgacca
     1021 ctcctggtcg cggtgcaggc catgtccggc gatgcccacc accccgcgga cgtgatcagg
     1081 gtgatcgagc gcgtatcgaa cggcgagatc ggagccccac gaatgtccaa gcacgatcca
     1141 ctgctcggca ccgatcgccc ggcgtagtgc ctccagatcg gcgacggcca gagccatgga
     1201 gtgtggcccc ccaccagaac gtccgactcc ccgtggatca gggaaccagc acctgaaacc
     1261 cgccggagcg atcgattcat cggcgaggta gtgcacacat cccggtcccc cggagagcac
     1321 cacgacatcg ggtccctcgc caccggaggc cgcgtgcagc actaccccgt catccgccgt
     1381 gacgaacatg gggaatcccc tcccagaaga cctcgacgtg gtggcgagtg cgcgccgacc
     1441 gccgagagca ggaccgccgt tagctcggca cccataggca gaacggatgt cccgccgggt
     1501 cgaatagcac gcgcacatca tcctgcggct ggtatgccgc gagcgtggct cccagcgcga
     1561 tcgcccggtc gacagcggct cccaggtcgc catccacccg gacttctaga tgcgcctgca
     1621 tctgttgggc ttcggcggtc tgcggccaga ccggcggctg atgggtgggc tcgcgctgca
     1681 cggacagcgc caccgagccg tccgaggcgg ccagatagtg ccagtcggga ttgtcctcgt
     1741 ccacggcata cccgagcagt tgggcgtaga agcgcgagag acccgggaca tcggcggccg
     1801 aaaggttgac cgtggagacg cgcatccgcg gggacgcacc cgaggctggc gggacgcacc
     1861 cacagcgcac ccgattgtga tcagggtcga cgagttgcac ctcgcgtgcc cacggcgcct
     1921 cgttcacgct caccccgaac tccgccgcga tccgatcgat gtcggggacg tagacataga
     1981 cgaggcctgg cgcgcgggcg tcacccgcgt gttcagagag gtggacgatc gcgtcgtcgc
     2041 gtctcagccc gacatagagc ggcaagtgcg gctcgaaacg gtgctcgaac tcgacctgga
     2101 accccagccg ggcccaccac gcgatcgcca cccgggcatc tcggacccgc agaacgggag
     2161 ccgcgttggc ccgcatcaga agacggacac ccggtcggcg gggtcgagcc acatgccgtc
     2221 gccctccccc acgccgaacg cctcgtggaa ggcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnagccggt tgaactgctc gacgagcttg gcaccgaggg cgtcgaaggc
     2401 ctcccgatca tcgtcggtcc accagttgcg caggttgccc gagccgtcgt attggctgcc
     2461 ttggtcgtcg aacccgtgcc cgacctcatg cccgatgact ccgccgatgc cgccatagtt
     2521 caccgcgtcg tcggcctcga cgtcgaagaa ggggggctgc aggatcgcgg cggggaagac
     2581 gatctcgttc atgcccgggt tgtagtaggc gttgaccgtc tgcgggctca tgaaccactc
     2641 gccacgatcg acgggccggc caagcttggc gaggctgcgg tcgctctcga aggcgttcgc
     2701 cgcggccacg tcagccagca ggtcgccggt gagcacgagg gcgctgtaat cgcgccacgt
     2761 ctcggggtag ccgatcttcg gcgtgaactg ctcgagtttg gcgagggcct gggctttggt
     2821 gtcctcgccc atccactgcc ggtcggcgaa cgagcgccgg aacgcctcga cgaggttgct
     2881 gacgagctcg ctcatccggg ccttggcagc cggcgggaaa tgccgggcga catagagctc
     2941 gcccagggcc tcgcccagcg tgcggtcgat caactcaaca ccgcgtttcc agcgctcgcg
     3001 cagttgcggc gtgccggcga gggtgcggcc gtagaagtcg aagttctcct cgacgaactc
     3061 ctgcgggagg aacggcgccc gcgagtggag gatctgccag gccagccagt cctgccacgt
     3121 cccgatcgac acctcggcca gcgcgtcgga cagggcacgc aggtagtccg gctggcgtac
     3181 gaccacttcg gcgagagcgt tgggcgaacc cagcgcagcg gcataccccg cccaatcgac
     3241 gtgcggcgcg aggtcggcga gcgcggcggc ggtgaacagg ttgtaggtgg cgaccgggtc
     3301 gcggtcggcg accttgtcga gatgacccgc ggccagccgg gtctccaggc tcaggacgcg
     3361 caccgcggcc gcctcgggct ccgggcgccc ggcgagggtc agcatcgcgg tcacatggcg
     3421 caggtatgcc gcccgcgtct cggcgtagct ctcctcgcgg tagtaggact cgtcgggcag
     3481 gccgagaccg ccctggtgga ggtagccgat gtagcgatcc gaggcgcggt catcggtgtt
     3541 gacgtagtgg tggaaggcac cgccgatgcc gatccggtcg agccgggcga gcagggccac
     3601 gaggtcgtcg gcgttggtca ccccatcgac ttcagccagc aggcccgcca cggctccgag
     3661 cccggcggcc tgcacggccg cctcatcgag gaacgcgcca tagagcaggg cgacccggcc
     3721 catcgacgta tcgggtgcgg cgcccgacgc cacggcctcg tcgaggatct cgcgcaggtc
     3781 atgctcggcc tgctcgcgca ggtggtcgaa ggtgccgtag cgacctcgat cggcggggat
     3841 ctcggtctgt gcgaaccacg tgccgttggt gtagccgaac aggtcgtcct ggggtcggat
     3901 cgacaggtcg aggttcgcgc gaatgattcc ggacgtcatg ccagtcatcc catcacaccc
     3961 gaccgacaac ccctgccggg tcgaggtatt ccaccatcgc caggtgacgg ccgaatacct
     4021 cgacccggca gagcggggca ggaggcggtc agtggccgag gagggtgtgg acggcgtcgg
     4081 ccagagcttc gggggtgcgg acgcacgccg cggtgtgcgc gtcgggccgg atcagccaca
     4141 actcctcggc caccgcctgc aagccggtgg ccacgacgtc ctcgtcaccg aggtccgaga
     4201 agtgcagcac ccgcaccggc agaccggccg gcacgacccc agacaacagc ccctcgacct
     4261 cgtgggtgtt gactgcctcg cccacgagga tgagcagccc ctcccgggcc aactcgcgca
     4321 cccggctctt gccatgcccg gcggaaaccg tcacggcatc ggggaggatg acgcccggaa
     4381 caggggtcgg tgcctgcccg cgaggcgggc gacccggcca gaaactgctc ggatcgggcg
     4441 tggtcaaggg gctgtcgacg taccagaagg cctcggccag tcggccggag tcgaccagcg
     4501 ggatgacctt cttgtccttg cgagcccgct tgaggatctc cttgcggcga tgcttggcct
     4561 tcttcgtctg cgggacaagg aagtccatcg tcgcggccgt caccgcgagg ttctcgcgag
     4621 cagcggcctg tcgctcgctc tcgtagctgc cgacgagccc aggaccagcc caccccttca
     4681 tggcgaaggc gatcttccag gcggcgttct cgacatccgg cacgccgctg ttgaggcccc
     4741 gagccccgaa cggcgccact aggtgggcga catcgcccgc gaggaggaac cgtccaaccc
     4801 ggaagcggtc tgcgctgcgc gcatggaagc gatagaccga cacccagtcg atccgatagt
     4861 cacgcttctt gccgatgacc ttacggatgc gcttgtccag cgacccgtcg gcctgcgcgg
     4921 ccgccaggtc gaagtcggtg tcgacctgcc agtcgatccg gaagatgctg ttcgggcacg
     4981 ggtggatgag cacctgacgc cccgggttcc actcggggtc gaagtagaac cggcgctcgt
     5041 tctcccaccc cttcaaagtg gtttcgatgt cgcagataag gaacgagtcg tcgaaggtcc
     5101 gccccgggaa ggtcaccccg agggcctccc gcaaggggcc acccttggaa ccggcgcaga
     5161 cgacggcata ctcccccgcg acctcgaact caccgaaacc gctgcggcac accaccgttg
     5221 ccaagccgtc actgtcgctc ttgcccccag cgacccccac gacctcggtc ccccaccaga
     5281 cctcgatgag cggttgcgca gcgatcgcct cgtcgaggat cgcctcggac cgggcctggg
     5341 agatgttgac gaagggcggc agcggggagc gccccgggtc ggggatctcc cagcagaaga
     5401 gttccttgtc cttgtagaag gtgcgcgcgc gcgaccaggt cagcccttcg gtcgcgacgg
     5461 caccagcccc gcaggcgtcc cacaggtcca ggacatcgcg ctgttgacac aacgccttgc
     5521 tcccgacggg gtcccgctcc ggccgtgagt ccagcacgag gacgggaatc ccccatcggg
     5581 ccagacatag cgccgcggtc tgaccgaccg ggccgttacc gatgacgagg acgcgcccgc
     5641 gcccctcggt gccgtgatcc tgcgacatgt cgctcctgcc gggagtggag taaccgatcg
     5701 tcggaggcta gcccacacgc tagccctgca gttgggccca aatctcctga tcgcgctcgg
     5761 cggtccagat cgtcggccgc tcgacgccct gcagttcttc ccacgtgcga gccacgtcga
     5821 acggcagggt gtgctcgaag atcggccagt gaccgtaggt cggcgacagc gcggcgtggc
     5881 acgcgtcgaa ggcctgcttg agggtgcccc ccgcggcctg cgtggcccgg acgttgtcga
     5941 tgattgacgt gatgaagcca cgggactgct cgatcgccgc gttcacggcc gcctcaccac
     6001 gagggatctc accgcgcccg ccgacgatgg cctcggcacc catcgccttg accgcgtcga
     6061 gcgtggtcga ggcccactcc tggtggaacg catcgccggt gtagagcgca gccttggcct
     6121 cgacgaggtc gccggcgaac atgatcttgt gcttgggcag ccaggc
//
LOCUS       DAOMZY010000217         6164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_11977_length_6164_cov_5.884760, whole genome shotgun sequence.
ACCESSION   DAOMZY010000217 DAOMZY010000000
VERSION     DAOMZY010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11977_length_6164_cov_5.884760"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PLK64_11830"
     CDS             complement(<1..759)
                     /locus_tag="PLK64_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV80483.1"
                     /translation="MQHQGALAGEVVAPVHDTGRGATRVLVMSTAAFTAMFAVWLMFG
                     ILGPSIQKEFGLTDVELSWITAVAVLNGSMWRLPAGMLTDRIGGRKVTVFMLAATAIP
                     AYFVSTASSYGMLLVLAFLVGFAGNLFSVGIAWNAAWYGRERQGFALGVFGAGNVGAS
                     VTKFIGPPIILGTAGSSFAFGLQGGWRLVPVIYSVLLVVLAVLTYIVVPRRDRMPGAT
                     KPVREMLTPLKQVRVWRFSLYYVAVFGAYVALSAW"
     gene            995..1747
                     /locus_tag="PLK64_11835"
     CDS             995..1747
                     /locus_tag="PLK64_11835"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV80484.1"
                     /translation="MSPEQIPVLSSDVRRRILEHLNVVAGQAPPGAPTGRSAADLAAY
                     LGLHVTTVRFHLDQLVSGGYLVTAFERTPRVGRPRKLYAAPHLDPDAETETQRHYQVL
                     AGLLAQSWPVDGGDQVTPEEAGRRWASGHADPSRASTEAAVTPGRWLAKVGDMVDQLE
                     HWGYTPELSTSAGGRAVTITLSNCPFLALARVRPDVVCGVHHGLITGALDHVGEHDVA
                     IEVRPFVGPTRCLVKVTTETPFTAAAPEGESR"
     gene            1744..5484
                     /locus_tag="PLK64_11840"
     CDS             1744..5484
                     /locus_tag="PLK64_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010552273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HPV80485.1"
                     /translation="MTTQVERPPAPEPTGLDGPLSDALIGTRRFFAKAKGATVSDDGR
                     TLHQIGGREGDSFYRDRWAHDKVVRSTHGVNCTGSCSWKVYVKDGIITWEAQQTDYPS
                     AGPDRPEYEPRGCPRGAAFSWYTYSPTRVRYPYVRGVLLEQFRAAKAVVGDPVVAWAA
                     IQQDPATRAAYTSARGKGGLVRASWDEVVEMVAAAHVYTVKRWGPDRIAGFSPIPAMS
                     PVSYASGARFLELIGAPMLSFYDWYADLPNASPQMFGDQTDVPESGDWWDAGYLIMWG
                     SNVPMTRTPDAHWMTEARYRGQKVVAVAPDYAENVKFADEWVPCRPGVDGALAMGMGH
                     VLLKEFFADRQVEFFEDYNRRYTDLPFLVSLDRHDETCYTAGKFVVAGDLDVPEKDSE
                     NALWKPVVLDARTDEVAVPHGSVGFRYGDEGLGKWNLDLGEIEPRLSMLGAHDDTALV
                     RFPRFDSEGGLVAYETRGVPVRTIDGRIVTTVLDLLLAHYGVARDGLPGQWPTGYADA
                     SVPATPAWAAEITGVPAEQITRLAREWAQNAIDTKGRGMILMGAGTNHWFHSDQIYRA
                     MLLLTTITGCQGRNGGGWAHYVGQEKVRPIMGFQHLAFALDWHRPPRHMNQTAYWYVN
                     TSQYRYDTFTADDLDAGTGVFAGKGVMDLLAQSVRLGWTPSYPTFNRSSLALADEAKA
                     AGQEPAAYVVEQLRTGALKFAVEDPEAPENHPRVLSLWRSNLLGSSAKGNEYFLKHLL
                     GTDHAVTAAEAAPDQRPDGIEWPEHAAEAKLDLLMTIDFRMTSSTMFSDIVLPAATWY
                     EKHDLSTTDMHPFVHSFSPAIDPPWEARTDWGIWKAIAKKFSSLAVDHLGTRTDVVAK
                     PLWHDTPEAMATVHGVVKDWRTGEVEPVPGKTLPVLAIAERDYPATFQKMTSIGPLLE
                     KVGMLTKGVPYDVKREIKILRRRNGVASSGVGAGQPRLKTDVHMADAILHLSATTNGH
                     LSTHGFKNVEKRTGTKLHDLAAEHEGKQVTFADTQVAPVPVITSPEWSGSESGGRRYS
                     PFTINIERLKPFHTLTGRQQFYLDHDWVIGMGESLPVHRPPLNMTTLFGETPIGGQSE
                     AHGQRSVSVRYLTPHNKWSIHSEYQDNLYMLSLSRGGQTIWMSDADADKVGINDNDWV
                     ELVNRNGVVSCRAIVTHRMPEGTIYMPHAQDRLVDVPLTETNQRRGGIHNSLTRIMIK
                     PTHIIGGYAQLAYFFNYIGPTGNNRDEVTMIRRRSSQEVTYE"
     gene            5481..>6164
                     /locus_tag="PLK64_11845"
     CDS             5481..>6164
                     /locus_tag="PLK64_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPV80486.1"
                     /translation="MKIMAQVAMVMNLDKCIGCHTCSVTCKQAWTNRAGVEYAWFNNV
                     ETRPGLGYPRGYENQDEWQGGWVRTSSGRLKLRSGGRFKKLAAIFSNPKLPSLHDYYE
                     PWTYDYDTVLNAPAQKNFPVARPFSLITGKPMNVSWSANWDDDLGGSRLHAVKDPMLK
                     GIEDKVQFEFEKTFMFYLPRICEHCLNPACAASCPSGAIYKRSEDGIVLVDQDRCRGW
                     RMCISGCPYK"
BASE COUNT         1109 a         2196 c         1954 g          905 t
ORIGIN      
        1 ccacgccgag agggcgacgt aggcaccgaa gacggccacg tagtagaggc tgaaccgcca
       61 gacccgcacc tgcttgaggg gcgtgagcat ctcgcgcacg ggcttggtgg ccccgggcat
      121 ccggtcacgc cgagggacga cgatgtaggt gaggacggcc aacaccacca acagcaccga
      181 gtagatgacg ggcacgagcc gccacccgcc ctggagcccg aacgcgaagg agctaccggc
      241 ggtcccgagg atgatcgggg ggccgatgaa cttcgtgacg gaggccccca cgttgccggc
      301 cccgaagacg ccgagcgcga agccctgccg ctcccggccg taccacgcgg cgttccacgc
      361 gatgccgacc gagaagaggt tgccggcgaa gccgacgagg aacgccagga caaggagcat
      421 gccgtagctg ctcgccgtcg agacgaagta ggccgggatc gcggtcgccg cgagcatgaa
      481 gacggtgacc ttgcggccac cgatgcggtc ggtgagcatc ccggccggca ggcgccacat
      541 cgacccgttg agcacggcca ccgccgtgat ccaggacagt tcgacgtcgg tgaggccgaa
      601 ctccttctgg atcgaggggc cgaggatgcc gaacatcagc cacaccgcga acatcgcggt
      661 gaaggctgcc gtggacatga ccaagactcg ggtcgcaccg cgaccggtgt cgtggacggg
      721 ggcaacgacc tctcccgcga gagcgccctg gtgctgcacg tcgatcactt ccttggggtt
      781 cctctaaatt acgccgagca gtccggttaa cgactcagaa aaacgctacc cccggcgcca
      841 accctccaaa ttcgcccgcg gtcctccaaa ctacgtcgcg ctagtcacac ctctgagcag
      901 ggcagatacg cacgaactga ggctcgccga cacagccgag tgtggtttga taagaacgca
      961 cccggattcc tcttaattaa ggagaccagg cctcgtgagc cccgagcaga tcccggtcct
     1021 gtcctccgac gtgcgccgac gaatcctcga acacctcaac gtcgtggccg gtcaggcgcc
     1081 ccccggagcg ccgaccgggc gcagcgccgc cgacctcgcg gcatacctcg ggttgcacgt
     1141 caccacggtc cggttccatc tcgaccaact cgtgtccggc ggctacctcg tcaccgcgtt
     1201 cgagcgcact ccgcgggtgg gacgcccccg caagttgtat gccgccccgc acctggaccc
     1261 ggacgccgag actgagaccc agcgccacta ccaggtgctg gccgggctgc tcgcccagag
     1321 ttggccggtc gatggtggcg atcaggtgac ccccgaggag gccggccgtc ggtgggcctc
     1381 cgggcacgcc gacccgagcc gcgcgtccac tgaggccgcc gtgactcccg gtcggtggct
     1441 tgccaaggtc ggcgacatgg tcgaccagtt ggagcactgg ggctacacgc cggagttgtc
     1501 gacctcggcc ggtggtcgcg ccgtcaccat cacgttgagc aactgcccct tcctcgccct
     1561 ggcccgggtg cgtcccgacg tcgtctgcgg cgtccatcac ggcctcatca ccggcgcgct
     1621 cgaccacgtc ggcgagcacg acgtcgccat cgaggtccgg cccttcgtcg gaccgacccg
     1681 ctgcttggtc aaggtcacca ccgagacccc attcaccgcc gccgctcccg aaggagagag
     1741 tcgatgacca cccaggtgga gcgtcccccc gcacccgagc cgaccggcct ggacggcccc
     1801 ctctcggacg ccctcatcgg cacgcgccgc ttcttcgcca aggccaaagg cgccacggtg
     1861 tccgacgacg gtcgcaccct gcaccagatc ggggggcgtg aaggcgacag cttctaccgg
     1921 gaccgttggg cccacgacaa ggtcgtccgc tccacccacg gcgttaactg caccgggtcc
     1981 tgctcgtgga aggtctacgt caaggacggg atcatcacct gggaggccca gcagaccgac
     2041 tacccgtcgg ccggcccgga ccgccccgaa tacgagccgc gcggctgccc tcgtggcgcg
     2101 gccttctcct ggtacaccta ctcccccacc cgggttcgct acccctacgt ccgcggcgtc
     2161 ctgctcgagc agttccgcgc cgccaaggcc gtcgtcggcg atccggtcgt cgcctgggcg
     2221 gcgatccagc aggatccggc aacgcgggcg gcatacacca gcgcgcgggg caagggcggg
     2281 ctggtgcggg cgagctggga cgaagtcgtc gagatggtcg ccgcagcgca cgtctacacc
     2341 gtcaagcgct ggggaccgga ccggatcgcg ggcttctcgc cgatccccgc gatgtccccc
     2401 gtgtcctacg cgtcgggcgc gcggttcctc gagctcatcg gcgcaccgat gctgtcgttc
     2461 tacgactggt atgccgatct gcccaacgcc tcgccgcaga tgttcggcga ccagaccgac
     2521 gtccccgaat cgggcgactg gtgggacgcg ggctacctca tcatgtgggg ctcgaacgtc
     2581 cccatgaccc gcacccccga cgcgcactgg atgaccgagg cccgctaccg gggccagaag
     2641 gtcgtggccg tggcaccgga ctacgccgag aacgtcaagt tcgccgacga gtgggttccc
     2701 tgccgaccgg gcgtcgacgg cgcgctggcg atgggcatgg gtcacgtcct gctcaaggag
     2761 ttcttcgccg accgacaggt cgagttcttc gaggactaca accgtcgtta caccgacctg
     2821 ccgttcctcg tgtcactcga ccgtcacgac gagacgtgtt acaccgcagg caaattcgtc
     2881 gtcgctggcg acctggatgt gcccgagaag gactccgaga acgcgctgtg gaagcccgtc
     2941 gtcctcgacg cccggaccga cgaggtcgcg gtgccccacg ggtcggtggg gttccgttac
     3001 ggcgacgaag gtctgggcaa gtggaacctc gacctcggcg agattgagcc ccgactctcc
     3061 atgctcggcg ctcacgatga cacggcgctg gtgcggttcc cgcggttcga ctccgagggc
     3121 ggcctggtgg cctacgagac ccgaggcgtg ccggtgcgca cgatcgacgg ccgcatcgtc
     3181 accaccgtcc ttgacctgct gctcgcacac tacggggtcg cgcgcgacgg actgccgggc
     3241 cagtggccga cggggtatgc cgatgcgtcg gttcccgcga cccccgcctg ggccgcggag
     3301 atcaccggcg tgcctgccga gcagatcacc cgactggcac gcgaatgggc gcagaacgcg
     3361 atcgacacca agggtcgcgg catgatcctc atgggtgcgg gcaccaacca ctggttccac
     3421 tccgatcaga tctatcgggc catgttgttg ctcacgacga tcaccgggtg ccaagggcgc
     3481 aacggtggcg ggtgggccca ctacgtcggc caggagaagg tccgcccgat catgggcttc
     3541 cagcacctgg ccttcgccct cgactggcac cggccgccgc gacacatgaa ccagacggcc
     3601 tattggtacg tcaacaccag ccagtaccgc tacgacacct tcacggccga cgacctggac
     3661 gccggcaccg gagtcttcgc gggcaagggc gtgatggacc tgctggccca gtcggtgcgc
     3721 ctgggctgga cgccgagtta tccgaccttc aaccgcagca gcctggccct cgccgacgag
     3781 gcgaaggcag cgggacagga gcccgcggca tacgtcgtcg agcaactgcg cacgggcgca
     3841 ctgaagttcg cggtcgagga cccggaggcg ccggagaacc acccccgtgt cctcagcctg
     3901 tggcggtcca acctgctggg ctccagcgcg aagggcaacg agtacttcct caagcacctg
     3961 ctcggcaccg accacgcggt caccgccgcc gaagccgccc ccgatcagcg acccgacggc
     4021 atcgagtggc cggagcacgc cgccgaggcc aagctcgatc tgctgatgac gatcgacttc
     4081 cggatgacga gctcgacgat gttctccgac atcgttctgc ccgccgcgac gtggtacgag
     4141 aagcacgacc tgtcgaccac cgacatgcat ccgttcgtcc actcgttcag cccggcgatc
     4201 gacccgccgt gggaggcccg caccgactgg ggcatctgga aggcgatcgc caagaagttc
     4261 tcatcgctcg cggtcgacca cctcggcacc cgcaccgacg tcgtcgccaa gcccctgtgg
     4321 cacgacacgc ccgaggcgat ggcgaccgtt cacggagtcg tcaaggactg gcgcaccgga
     4381 gaggtggagc cggtgccggg caagacgctg ccggtcctcg cgatcgccga acgggactac
     4441 ccggcgacct tccagaagat gacctccatc ggcccgctgc tggagaaggt cggcatgctc
     4501 accaagggcg tgccctacga cgtcaaacgc gaaatcaaga tcctccggcg ccgcaacggg
     4561 gtcgccagct cgggggtcgg cgccggtcag ccacgcctca agaccgacgt gcacatggcc
     4621 gatgccatcc tgcacctgtc ggcgacgacc aacgggcacc tctcgacgca cggcttcaag
     4681 aacgtcgaaa agcgcactgg cacaaagctg cacgacctgg ccgccgagca cgagggcaag
     4741 caggtgacct tcgccgacac ccaggtcgcg ccggtccctg tcatcacctc gccggaatgg
     4801 tcgggctccg agtcgggcgg acgtcgctac agcccgttca ccatcaacat cgaacggctc
     4861 aagcccttcc acaccctgac cgggcgacaa cagttctacc tcgaccacga ctgggtcatc
     4921 ggcatgggcg agtcgctgcc ggtgcaccga ccgccgctca acatgacgac cctgttcggc
     4981 gagaccccca tcggcgggca gtccgaggcg cacgggcaac gcagcgtctc agtccgttac
     5041 ctgacgccgc acaacaagtg gtcgatccac agcgagtacc aggacaacct ctacatgctc
     5101 tcgctctccc gcggcgggca gacgatctgg atgtccgacg ccgacgccga caaggtcggc
     5161 atcaacgaca acgactgggt cgagttggtc aaccgcaacg gggtcgtctc gtgccgtgcc
     5221 atcgtcaccc accggatgcc cgaaggcacg atctacatgc cgcacgcgca ggaccggttg
     5281 gtcgacgtgc ctctcaccga gaccaaccag aggcgaggcg gcatacacaa ctcgctgacc
     5341 cgaatcatga tcaagccgac gcacatcatc ggtggctacg cccagttggc ctacttcttc
     5401 aactacatcg gccccaccgg gaacaaccgc gacgaagtga cgatgatccg ccgtcgctcc
     5461 tcgcaggagg tgacctacga atgaagatca tggctcaggt agcgatggtc atgaacctcg
     5521 acaagtgcat cggctgccac acctgctcgg tgacctgcaa gcaggcgtgg accaaccgcg
     5581 cgggcgtgga atatgcctgg ttcaacaacg tcgagacccg gccgggcctt ggctatcccc
     5641 gcggctacga gaaccaggac gagtggcagg gcggttgggt acgcacctcg tcgggtcggc
     5701 tgaagttgcg ctccggcggt cggttcaaga agctcgccgc gatcttctcc aaccccaaac
     5761 tgccgtcgct gcacgactac tacgagccgt ggacctacga ctacgacacg gtgctcaacg
     5821 ccccggccca gaagaacttc cctgtcgcac ggcccttctc gctcatcacg ggcaagccga
     5881 tgaacgtctc gtggtcggcc aactgggacg acgacctcgg cggctcgcgc ctgcacgccg
     5941 tcaaggaccc gatgctcaag ggcatcgagg acaaggtcca gttcgagttc gagaagacgt
     6001 tcatgttcta cctcccgcgg atctgcgagc actgcctcaa ccccgcgtgc gctgcgtcgt
     6061 gcccgtccgg tgcgatctac aagcgctccg aagacggcat cgtgctcgtc gaccaggacc
     6121 gctgccgcgg ctggcggatg tgcatctcgg gctgcccgta caag
//
LOCUS       DAOMZY010000218         6150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_12006_length_6150_cov_6.295488, whole genome shotgun sequence.
ACCESSION   DAOMZY010000218 DAOMZY010000000
VERSION     DAOMZY010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12006_length_6150_cov_6.295488"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="PLK64_11850"
     CDS             complement(<1..1319)
                     /locus_tag="PLK64_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006214020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HPV80487.1"
                     /translation="MSEHGGFTDEESYLDDDEGNGSVKGVIRIASVAALGGFLFGYDS
                     AVINGAVAPIEEHFDVAPSVLGFAVASALLGAAAGALSAGRIADRIGRLTVMKIAAVL
                     FFVSAIGTGLAPNLPLLIFFRIVGGVGVGVASVIAPAYIAEISPARIRGRLGSLQQLA
                     IVTGIFLSLLIDFALAEMAGGADQVLWLGLEAWRWMFIAMVIPALAYGLLALTIPESP
                     RYLVSRHRIPEARKILTALLGEKNLDLTIDRIQRSLDRDTPPAWRDLKSPAGSLYPIV
                     WVGLALSIFQQFVGINVIFYYSNILWQAVGFSESGSFVITVITSVINIVTTLIAIASV
                     DRFGRKPLLLIGSAGMAVTLATMAVIFGTASVNAEGNPVLEGAAAPIALVAANLFVVA
                     FGMSWGPVVWVLLGEMFPNRIRGAALSMAAAGQWVANWLITVSFPSL"
     gene            complement(1491..2690)
                     /locus_tag="PLK64_11855"
     CDS             complement(1491..2690)
                     /locus_tag="PLK64_11855"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HPV80488.1"
                     /translation="MPHGKSSPQPGSPSALRRANRQRILSLLETGDELSQADLARATG
                     LAPATVSNIVRELLADRNLVVAGRSGRAHTLRLDAGSGIAVGIDVGHRHITVATVSRA
                     GEVLAERRADTRTNLPADDAVRIMKAAFGEVLAETGIREEALLGIGMGIPTPIDQGTR
                     RVAAPSILPGWVGVDMGELVSQQLGREVVVDNDANVGALAELTWGAGRGLESLAYIKL
                     SEGVGAGLILGGELYRGRHGIAGEIGHTTMDEFGSVCRCGNRGCLETLVAARTVINLL
                     EPVRGHDLTISDIVRWAQEGDVACARVLADTGRHVGVALANICNVFNPERILIGGELA
                     LAGQFLLTPIREVIRRQGIPAATESLTIEVSALGAHTHVLGAALLAIHTAPTTSLHKM
                     NIEDTSA"
     gene            complement(2766..3692)
                     /locus_tag="PLK64_11860"
     CDS             complement(2766..3692)
                     /locus_tag="PLK64_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020012993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HPV80489.1"
                     /translation="MSEQATVGFALVDVFASTPLTGNPAAVVPDADALSLDQMQALAR
                     EFNQSKTVFIVAVDSRFTGDSGSQGGSGLEADWQLRSFTPIGAEVGGAGHNALAACLW
                     LVDRELAADRTEFGVQIGTDLLAVKVTRPDGEPAHVTIAQSAPEFGATLDDRAALVDA
                     LGLTTADLSDDTVQVVSTGAGHLMVPLIDRATVDRVQPDTRRLAEVLRTVAGEGCYVY
                     SLDTVEGDSAAYARFFNPTMGIVEDAATGTAAGPLIAKLVAEGRVARGVSAVVEQGHT
                     LGRPSRILITVDGDAVSISGAGLIVASGTVLV"
     gene            complement(3685..4572)
                     /locus_tag="PLK64_11865"
     CDS             complement(3685..4572)
                     /locus_tag="PLK64_11865"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HPV80490.1"
                     /translation="MSTASSGVATLPVAPVTGPAAQTAWVLALGGASLLPDLDTTGST
                     AARMWGPITRTLGTLVGAIAQGHRQGTHDVVLAPLAFASAALLASLHPVTTGAVLAVT
                     IGLTLRGLALAGVGRIGAAANLLVSAIAAWILVAAGAHQIRLLPLVLAAGVLIHIAGD
                     WLTDEGVPIPILWILGHRRRLSLGLFGVNGPLERALIAPLLSLAGVLLLCSHLGIHDV
                     DSLARWCGTLLQQLPDSDDRLRDGDEGVGDERDHHGQQALAPGGFLRFAPAFARSCHL
                     CTLARLPVMGRRGVRRFDV"
     gene            complement(4629..6086)
                     /locus_tag="PLK64_11870"
     CDS             complement(4629..6086)
                     /locus_tag="PLK64_11870"
                     /inference="COORDINATES: protein motif:HMM:NF015043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HPV80491.1"
                     /translation="MTNRYLRDNFAPVHEEITSFDLDVTGRVPDHLDGRYLRIGPNPI
                     GSPGEGHHWFMGDGMVHGVRLAESDARWYRNRYVRSGSVARHLGEPSRSEGWESTDFA
                     PNTHVLEHAGHTLALVEGGSSPYVLTEELDTVGPTDFEGTLQMMGTLPAGYSAHPHQD
                     PETGELHAVSYNWLRGNQVDYTVRDTQGRVRRHVPIQVGGSPMMHDFALTENYVVIYD
                     LPVVFDKRRALQGMPLAARIPTRLMMAAIIGRNPLPDPVISRIARGSYAGGSSATLPY
                     SWDPGYPARIGLLPREGTDTDTRWFDIDPCFIFHTLNAYEDGDTVVIDVVRHDRMFAT
                     VLNGPDEGPPTLARFTLDLSGDKVREDRFDDHAQEFPRFDERRTGRRHRFGYSVGFQG
                     GLPGDSVLRHDLAAGSTLVRSLGTGREASEFCFVANDGTTDEADGVLMGYAFDRASGT
                     SDLVILDAVTLEDVAAVHLPARVPAGFHGSWNPTT"
BASE COUNT         1036 a         1955 c         2222 g          937 t
ORIGIN      
        1 ttcagcgagg ggaagctcac cgtgatgagc cagttcgcca cccactggcc ggcggcggcc
       61 atcgagagcg cggcgccgcg gatgcggttg gggaacatct cgcccaagag gacccagacg
      121 accggacccc aggacatgcc gaacgccacg acgaacaggt tggcggcgac cagggcgatg
      181 ggggcggctg caccctcgag caccgggttg ccctcggcat tcacggaggc ggtcccgaag
      241 atcaccgcca tggtggccag ggtgacggcc atgcccgcag aaccaatgag cagcaacggt
      301 ttgcgaccga agcggtccac ggaggcgatg gcgatgagcg tcgtgacgat gttgatcacc
      361 gaggtgatga cggtgatgac gaacgagcca ctctcggaga atccgacggc ctgccacagg
      421 atgttggagt agtagaagat gacgttgatg ccgacgaact gctggaagat cgagagcgcc
      481 agaccgaccc agacgatcgg atagagcgag cccgcaggtg acttgagatc cctccaggct
      541 ggcggggtgt cacggtcgag actgcgctgg atgcggtcga tcgtgaggtc gaggttcttc
      601 tcaccgagca gggccgtgag gatcttgcgc gcctcgggga tgcggtgtcg cgacacgaga
      661 tagcgcggcg actccgggat ggtcagggcg agcaggccat aggcaagtgc cgggatgacc
      721 atggcgatga acatccaccg ccaggcctcg aggccgagcc acagcacttg gtcggcgccg
      781 ccagccatct cggcgagggc gaagtcgatg agcagcgaca ggaagatgcc ggtgacgatc
      841 gcgagttgct ggagggagcc gaggcggccg cggatccggg ccggtgagat ctcggcgatg
      901 taggcaggtg cgatcaccga ggcgacaccg acaccgacgc cgccgacgat ccggaagaag
      961 atgagcagcg ggaggttggg cgcgagaccg gtgccgatcg cgctgacgaa gaagagcaca
     1021 gcagcgatct tcatgacggt gaggcgaccg atgcggtccg cgatccgacc ggcactcagc
     1081 gcgccggcgg cggcgccgag cagggccgaa gcgaccgcga agccgaggac acttggggcg
     1141 acgtcgaagt gctcctcgat cggggccacg gcaccgttga tgacggcgct gtcgtagccg
     1201 aagaggaagc cgccgagcgc agcgaccgag gcaatcctga tcacgccctt gacggagccg
     1261 ttgccctcgt cgtcatcgag gtacgactcc tcatctgtga atccaccatg ctcgctcatc
     1321 ggcacccttc cgtggggcca ccacacgatc cgcccgtccc ggaagtgcgt gtctgtgcct
     1381 gtctccggtg acaagcggcc cgggcgtgac cgggacgact tcgttgtcgt tgattcgaca
     1441 aatggtagtt tatcgacgcg cgtcgcactt gacaagaggt gaacgaaact ttacgcggat
     1501 gtgtcttcta tgttcatctt gtgaagactc gtagtaggtg ccgtatggat cgcgagcaag
     1561 gccgcgccga gcacgtgagt gtgcgctccg agggcgctca cctcgatcgt gagtgactcc
     1621 gtcgcggccg ggatgccctg acgacggatg acctcgcgga tgggggtgag gaggaactga
     1681 ccggccagcg ccaactcgcc accgatgagg atgcgctcgg ggttgaagac gttgcagatg
     1741 ttggccagcg cgaccccgac gtgccggccg gtgtcggcga gcactcgggc gcaggcgaca
     1801 tcgccctcct gcgcccaccg aacgatgtcc gaaatcgtga ggtcgtgtcc gcggaccggc
     1861 tcgagcaggt tgatgaccgt gcgggcggcg accagcgtct cgaggcagcc gcggttgcca
     1921 cagcgacaga cgctgccgaa ctcgtccatc gtggtgtggc caatctcgcc ggcgatgccg
     1981 tggcgtccgc gatagagctc gccgccgagg atgagtccgg caccgacacc ctccgagagc
     2041 ttgatgtagg caagggactc cagaccgcga ccggctcccc aggtgagctc agcaagtgct
     2101 cccacgttgg cgtcgttgtc gaccacgacc tcacgcccga gctgctgcga gacgagctcg
     2161 cccatgtcga cgccgaccca gccgggcagg atcgagggag ccgccacccg gcgcgtcccc
     2221 tgatcgatcg gcgtggggat gcccatgccg atcccgagta gcgcctcctc gcggatgcct
     2281 gtctcggcga gcacctcgcc gaaggccgcc ttcatgatgc gcaccgcgtc atcggccggc
     2341 aggttggtgc gggtgtcggc tcgccgctcg gccagcacct caccggcccg actcacggtc
     2401 gcgacggtga tgtgccgatg cccgacgtca atgcccacgg cgatgcccga gccggcgtcg
     2461 agcctgaggg tgtgggcgcg tccgctgcga ccggcgacga caagatttcg atcagcgagc
     2521 agctcgcgca cgatgttgga caccgtcgcg ggcgcgagcc cggtggctct tgccaggtcg
     2581 gcctggctga gctcgtcgcc cgtctccagc agggagagga tgcgctgccg attagccctg
     2641 cgcagggccg atggcgaccc aggttggggg gaggatttcc cgtgcggcat ggctacatcc
     2701 tgaacgacag tggcgaggat tcgtcaatct acttcactcg ggcggcggcg cgaccgccgt
     2761 cgcagttaga ccaggaccgt cccgctggcg acgatcagac cggccccgct gatcgacacg
     2821 gcgtcgccat cgacggtgat gaggatccgg ctcggtcgac ccagggtgtg cccctgctcg
     2881 acaacggctg acacgccgcg cgccacgcgt ccctctgcca ccaacttggc gatgagtgga
     2941 cccgccgccg tgccggtcgc cgcgtcctcg acgatcccca tcgtgggatt gaagaagcgg
     3001 gcgtatgccg cagagtcacc ttcgaccgta tccaggctgt agacgtaaca gccctctcct
     3061 gcaacggttc tgaggacctc ggccaggcga cgcgtgtcgg gttgcacccg gtcgacggtg
     3121 gcgcggtcga tgagcggaac catgaggtgg ccggctccgg tcgagaccac ttgcacggtg
     3181 tcgtcgctca ggtcggcggt ggtcaggccg agcgcgtcga ccagtgccgc tcggtcatcg
     3241 agggtggcgc cgaactccgg agcggactgc gcgatcgtca cgtgggccgg ctcaccgtcg
     3301 gggcgggtga ccttcaccgc cagcaggtcg gtgccgatct gcacgccgaa ctcagtgcga
     3361 tcggcagcga gctcccggtc gacgagccag aggcacgcgg ccagcgcgtt gtgcccggcc
     3421 ccgcccacct ctgcgccgat gggtgtgaac gagcgcaact gccaatcggc ctccaacccc
     3481 gagccgccct gagaccccga gtcgcccgtg aaccgcgagt cgaccgcgac gatgaagacg
     3541 gtcttggact ggttgaactc ccgcgccaac gcctgcatct ggtccagcga caacgcatcg
     3601 gcgtcaggca cgacggcggc cgggttcccg gtgagcgggg tggacgcaaa gacgtcgacg
     3661 agggcgaacc ccacggtcgc ttgttcagac atcgaatctc ctcacgccac gacggcccat
     3721 gactggcaat cgtgccagag tgcagaggtg acaggaccga gcgaacgccg gggcgaagcg
     3781 caggaatcca ccgggggcga gagcctgctg accgtgatga tcgcgctcat cgccaacacc
     3841 ctcatcgcca tcgcgaagtc tgtcgtcgct gtcagggagc tgctgcagca gagtgccgca
     3901 ccagcgcgcg agggagtcga cgtcgtggat gccaaggtgg gagcacagca gcaggactcc
     3961 ggcgagcgag agcagtgggg cgatcagcgc gcgctcgagg ggcccgttga ccccgaacag
     4021 gccgaggctg agtcgacgac ggtgaccgag gatccagagg atcgggatcg ggacgccttc
     4081 gtcggtgagc cagtcgccgg cgatgtggat cagcaccccg gcggccagga ccaggggcag
     4141 gagccggatc tgatgggcgc cggcggcgac caggatccag gctgcgatcg cggacacgag
     4201 caggttggcg gccgctccga tgcggccgac gccggccagg gcgagcccgc gcagggtcag
     4261 gccgatggtc accgccagga ccgctccggt ggtgaccggg tggagcgagg ccaggagcgc
     4321 ggcgctggcg aaggcgagcg gcgccaggac gacgtcgtgg gttccttgac ggtggccttg
     4381 ggcgatcgcg ccgacgaggg tgcccagagt gcgcgtgatc gggccccaca tgcgggcggc
     4441 ggtggatccg gtggtgtcca ggtcgggcag caggctggcg ccacccaggg cgagcaccca
     4501 tgcggtctgg gcggctgggc cggtgacggg cgcgaccggc agggtggcga cccccgagct
     4561 ggcggtcgac aggcggcggt aggacgcagg ttcgaagcct ggcgcactgt ctcggcgttg
     4621 ctcgtgcgct aggtggtggg gttccagctg ccgtggaaac cggcggggac acgcgcgggt
     4681 agatggacgg cggcgacgtc ctcgagcgtc accgcgtcga ggattaccag gtcgctggtg
     4741 ccggaggccc gatcgaacgc gtatcccatc agcacgccgt cggcctcatc agtggtcccg
     4801 tcgttggcga cgaagcagaa ctcgctcgcc tctcggccgg tgccgaggct ccgtacgagg
     4861 gtcgaaccgg ccgcgaggtc gtgacgcagc accgagtcgc cgggcaagcc cccttggaag
     4921 cccaccgagt agccgaagcg gtgtcgcctc ccggtccgcc gctcgtcgaa gcgcgggaac
     4981 tcctgcgcgt gatcgtcgaa gcggtcctcg cgaaccttgt cgccactgag gtcgagggtg
     5041 aaccgtgcca gtgtcggtgg tccttcgtcg ggcccgttga gcactgtcgc gaacatccgg
     5101 tcgtggcgca ccacgtcgat gacgactgtg tcgccgtcct cgtaggcgtt cagcgtgtgg
     5161 aagatgaagc acgggtcgat gtcgaaccat cgggtgtcgg tgtcagtgcc ctcgcgaggc
     5221 agcagtccga tccgtgcggg gtagcccggg tcccacgagt agggcagggt cgcgctcgag
     5281 ccgccggcgt aggagccgcg ggcgatccgc gagatcaccg ggtctggcaa cgggttgcgc
     5341 ccgatgatgg cggccatcat cagcctcgtg ggaatccggg cggccagagg catcccttgg
     5401 agggctcgtc gcttgtcgaa gaccacgggc aggtcgtaga tcactacgta gttctcggtc
     5461 agagcgaagt cgtgcatcat cgggctgcca ccgacctgga tcggcacgtg gcggcgcacg
     5521 cgtccctgcg tatcgcgcac cgtgtagtcg acctggttgc cccgcagcca gttgtaggag
     5581 accgcgtgga gttctccggt ctcggggtct tggtgtgggt gtgcggagta gccggccggc
     5641 agggtgccca tcatctgcag ggtgccctcg aagtcggtcg gccccaccgt gtcgagctct
     5701 tcggtcagca catagggcga ggagccaccc tccaccaggg ccagggtgtg gcccgcatgc
     5761 tccaggacgt gggtgttagg ggcgaagtcg gtgctttccc aaccctcgga tcgggacggt
     5821 tcgccaaggt gccgggccac gctgcccgag cgcacgtagc ggttgcgata ccaccgggcg
     5881 tcgctctcgg cgaggcgcac cccgtgcacc atcccgtcgc ccatgaacca gtggtgccct
     5941 tcgcccggtg acccgatcgg gttcgggccg atgcgcagat agcggccgtc gagatggtcg
     6001 ggcacccgac cggtcacgtc cagatcgaag ctggtgatct cttcgtggac gggggcgaag
     6061 ttgtccctca agtagcggtt ggtcatgacg cgtctcctta gcctcgatcc gtaagttggt
     6121 gggtggcccg ggagggtcgg atgtggctga
//
LOCUS       DAOMZY010000219         6100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_12136_length_6100_cov_6.132175, whole genome shotgun sequence.
ACCESSION   DAOMZY010000219 DAOMZY010000000
VERSION     DAOMZY010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12136_length_6100_cov_6.132175"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1822)
                     /locus_tag="PLK64_11875"
     CDS             complement(101..1822)
                     /locus_tag="PLK64_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HPV80492.1"
                     /translation="MSPLRMGSATVTVVTAAELADRAAALSHACTEGARELEPAQVDQ
                     AVNLVDKVRERTSIAGGHTVVALAGATGSGKSSLFNAIVGEQVARIGARRPTTSVPSA
                     AVWGTTSATELLDWLSVSSRHQVDGATRMDDSDAAGSDVVGSDGVAVTGPAGSLDGLV
                     LLDLPDFDSRVDAHRIEAQRVLELVDIFVWVTDPQKYADALLHEDYVRALATHDAVTV
                     VVLNQIDRLTPEAVSACRDDLRRLLVADGLAQARVFATSARTGTGVPELVQQLANAVS
                     GANATRHRLSADVVSVAGKLRAGVADNEIDLDGRADPGLVEALSRAAGVPIVLDAVAA
                     DYRRESRQAGGWLFTRWLSNIKADPLARLRLDKTVVPMKSVGEADVRAVLGRSSIPPP
                     SPAARAAVSLATRQLADRASEGLPVRWAHAVADASATGDALTDSLDQAILHTPLRARP
                     PRWWTLVGTLQWIFGAAAITGALWLTALAVVAWLQLPDLPTPTVWAVPWPFLLLAAGV
                     VLGLVTAGLSRLAARVGARRRAAKARKRLHDAISAVALDRIVGPIAAVLARHARVREF
                     LDRAAAR"
     gene            complement(1819..3498)
                     /locus_tag="PLK64_11880"
     CDS             complement(1819..3498)
                     /locus_tag="PLK64_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HPV80493.1"
                     /translation="MLLAAVNGLRDQVEASRLPLALPDAPAAGKVRLELLQQLDDYVI
                     PRLSSLDAPLLAVVGGSTGAGKSTLVNSIVGMQVTRPGVLRPTTRSPVLVHHPSDEHW
                     FVDTRVLPGLARVRGGDLAEDQSAVRLVANDALPAGLALLDAPDIDSVVASNRDLAAQ
                     LLSAADLWLFVTTAARYADAVPWTLLRQASERGTSVAIILDRVPPEAVGEIRDHLTAM
                     LKEQGLALAPLFPIPEVPLGADGLLGDAEVGRLKGWLSALANDARARALVVRHTLSGA
                     LDSIDSRIAVLAEATGEQQAATNALARVADAAYAEAADGVRKGMLDGTLLRGEVLSRW
                     QEFVGTGEFFKQVESAVSRMRDRITAAVKGQPAPGQQLGEALQTGVAALVTAEGSAAA
                     SQVARSWRGMPGGQLLLDATPALAVPSADLTTQIETLVREWQDDVLDLVRNEGRDRRT
                     TAKVAAYGVNGLGVVLMLVTFAHTAGLTGAEAGIAGGTAVLGQKLLEAIFGDQAVRSL
                     AAKARAALLDRVEDLYAAESGRFHAAIDAVSVERDQGRKLSAAALNVRAAR"
     gene            complement(3676..3748)
                     /locus_tag="PLK64_11885"
     tRNA            complement(3676..3748)
                     /locus_tag="PLK64_11885"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3713..3715),aa:Arg,seq:tct)
     gene            complement(3876..5072)
                     /locus_tag="PLK64_11890"
     CDS             complement(3876..5072)
                     /locus_tag="PLK64_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HPV80494.1"
                     /translation="MSTGGSGVPGWDSGTAAYPMPGPGVPNPTRRPERRGWVTTGVII
                     SVFLISLAVLSVFFGIDIGIGNSLVAAAVSAVPLLVVVPVFLWVDRMEAEPVRYLIFA
                     FLWGALCAAVGAIILNTGIHLIFLAAHTDNADAVTAVVSAPLVEETLKGAAILFIAWR
                     RRREFDGVIDGIVYAGMAGAGFAFSENILYLGRAFAEAGTSGLTEVFFLRCLMGPFAH
                     PLFTACTGIGIGLAATVARSAAARIAYGTVGLVVAMALHAIWNLSASVGAYLQVYLAF
                     QMPIFAVFLVGLVLLRQREAGLVRRYLSQYADAGWFTRAEVEMLSSMGQRRSARAWAR
                     GLGGRAAVRSMRAFQDSASDLALLRARIVRGSAEPGAGAHERDLLDAVTANRGVVVGQ
                     VSPRDR"
     gene            5133..5837
                     /gene="orn"
                     /locus_tag="PLK64_11895"
     CDS             5133..5837
                     /gene="orn"
                     /locus_tag="PLK64_11895"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HPV80495.1"
                     /translation="MSTNASTTPTSSPTAGSSGGDRALADRIVWIDCEMTGLSLQADA
                     LVEVAALVTDYELTVLGEGVDVVICPPPESVAQMDPFVVDMHTTSGLLTELPTGVSLR
                     EAEERVLAYVREWVPEPRKAVLAGNTVGTDRTFLARDMPELEGWLHYRVIDVSSIKEL
                     SRRWYPRAYHNSPSKSGGHRALADIRESIAELRYYREAVFVPQPGPDSATARALGERH
                     VVVHPAVNSTVADPVR"
     gene            5871..5946
                     /locus_tag="PLK64_11900"
     tRNA            5871..5946
                     /locus_tag="PLK64_11900"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5904..5906,aa:His,seq:gtg)
BASE COUNT         1004 a         2213 c         2060 g          823 t
ORIGIN      
        1 cgcggcatac cccatcgcca gcctgtggac gatgggacga acgaccacgg gggtgtggac
       61 aacacccacc gcacgagccc gacgtcgcgc gaccctcggg tcagcgcgcg gcggcccgat
      121 cgaggaactc tcggacccgg gcatgtcgag ccaacacggc agcgatcggc ccgacgatcc
      181 gatccagggc cacggcggag atcgcatcgt gcagccgttt gcgggctttc gcggcccgtc
      241 gtcgagcacc gacccgggct gccaggcggg acagcccggc ggtcaccaac ccgagaacca
      301 cgcctgccgc caacagcagg aacggccagg gcaccgccca taccgtcggc gtcggcaggt
      361 ccgggagttg cagccaggcg accacggcga gcgcggtcaa ccacagggcg cccgtgatcg
      421 cagcggcgcc gaagatccat tgcagcgtgc cgacgagcgt ccaccagcgg ggcggccggg
      481 cacgcagcgg cgtgtgcagg atcgcctggt cgagcgagtc ggtcagcgcg tcaccggtcg
      541 ccgacgcgtc agcgacggca tgggcccagc gcacgggaag gccttccgag gcccggtcgg
      601 ccaattgccg ggtcgcgagc gagacggcag ctcgggctgc tggggacggc ggcgggatcg
      661 acgaccgacc cagcacggcc cgcacgtcgg cctcccccac cgacttcatc ggcacgaccg
      721 tcttgtccaa gcgcagtcgg gcgagggggt cggccttgat gttggagagc caacgggtga
      781 acaaccaccc acccgcctgg cgtgactcgc ggcgatagtc ggccgcaacg gcatccagca
      841 cgatggggac gcccgcggcc ctggacagcg cctcgaccag ccccgggtcg gctcgtccgt
      901 cgaggtcgat ttcgttgtcc gccaccccag cgcgcaactt ccctgcgacc gacacgacat
      961 ctgcggacaa ccggtggcgg gtcgcattgg caccggacac ggcgttggcc aactgctgaa
     1021 ccaactcggg caccccggtg ccggtgcgtg ccgaggtcgc gaagacgcgg gcctgggcca
     1081 ggccgtcggc gaccaggagg cgccgcaggt cgtcgcggca ggcggacacc gcctcgggag
     1141 tgagtcggtc gatctggttc aacacgacga cggtcacggc gtcgtgggtg gcgagcgcac
     1201 ggacgtagtc ctcgtgcagc agggcgtcgg catacttctg cgggtcggtg acccagacga
     1261 agatgtcgac gagctccaag acccgctgcg cctcgatgcg gtgcgcgtcg acccgcgagt
     1321 cgaagtcggg caggtcgagc agcaccagac cgtccaagct tccggccggg ccggttaccg
     1381 ccaccccgtc tgaacccacc acgtccgaac ccgccgcatc ggagtcgtcc atccgcgtgg
     1441 cgccatcgac ctggtggcgc gaggacaccg agagccagtc gagcagttcg gtggccgacg
     1501 tcgtgcccca caccgccgcg gacggcacgg aggtcgttgg gcgacgggcg ccgatccggg
     1561 ctacctgctc accgacgatg gcgttgaaca gggacgactt gccgcttccg gtcgcacccg
     1621 ccagggccac cacggtgtgg ccgccggcga tcgacgtccg ttcccgcacc ttgtcgacga
     1681 ggttgacggc ttggtcgacc tgggccggct cgagctcgcg ggcgccctcg gtgcaggcgt
     1741 gcgacagagc agcggcccga tcggccaact ccgcagcagt cacgaccgtc accgtggcac
     1801 ttcccatccg caaggggctc atcgggcggc tcggacgttg agggcggcgg cagacagctt
     1861 gcggccctga tcgcgctcga ccgacaccgc gtcgatggcg gcgtggaacc gtcccgactc
     1921 ggcggcatac aggtcctcga cccgatccag caacgccgcc ctcgccttgg ccgcgagcga
     1981 ccgaacggct tggtcaccga agatcgcctc gagcaacttc tggccgagga ccgccgtccc
     2041 gccagcgatg ccggcctccg cgccggtcaa cccggcggtg tgggcgaagg tgacgagcat
     2101 gaggacgacg ccaagaccgt tcacgccata ggccgcgacc ttggccgtcg ttcggcgatc
     2161 gcggccctcg ttacggacga ggtccaggac atcgtcctgc cactcccgaa ccagggtttc
     2221 gatctgggtc gtcaggtccg cggacggcac cgccaacgcg ggggtcgcgt cgagcagaag
     2281 ctgaccaccc ggcatacccc gccaagagcg agccacctgc gaggccgccg ccgagccctc
     2341 ggccgtgacg agcgcagcga cacccgtctg cagcgcctcg ccgagttgct gaccaggagc
     2401 gggctgcccc ttgaccgcgg ccgtgatccg atcgcgcatc cgcgagaccg cggactccac
     2461 ctgcttgaag aactcccccg tgcccacgaa ctcctgccag cgcgacagca cctcgccgcg
     2521 caggagggtc ccgtcgagca ttcccttgcg caccccgtcg gctgcctcgg cgtatgccgc
     2581 gtccgcgacc cgcgcgagcg cgttcgtcgc cgcctgctgc tcgccggtcg cctccgcgag
     2641 gacggcgatc cgcgagtcaa tcgagtccaa ggcgccgctg agggtatgcc gcaccacgag
     2701 ggcccgcgcg cgggcgtcgt tggcgagtgc gctcagccag cccttgagac ggccgacctc
     2761 ggcgtcaccc aagagtccgt cggcgcctag cggcacctcg gggatgggga acaacggcgc
     2821 cagtgccaaa ccctgctcct tgagcatcgc cgtgaggtgg tcacggatct cgccgactgc
     2881 ctccggcggc acccggtcga ggatgatcgc caccgacgtc ccgcgctcgg atgcctgacg
     2941 caacagcgtc cacggcacgg cgtcggcata acgtgcggca gtcgtcacga acagccagag
     3001 gtcggccgcc gagagcaact gggcggcgag gtcacggttg ctcgccacca ccgagtcgat
     3061 gtcaggcgcg tccagcagcg ccaacccggc tggtagtgcg tcgttggcga ccagacgcac
     3121 cgcggactgg tcttccgcga gatcaccgcc acggacccgg gccaggcccg gaagcacgcg
     3181 cgtgtcgacg aaccaatgct cgtcggacgg gtggtggacg aggaccggtg accgcgtcgt
     3241 cggacgcaac acccccggcc tggtcacctg catcccgaca atcgagttga cgagggtcga
     3301 cttaccggca ccggtcgatc ctccgacgac cgccaacagg ggcgcgtcga gggagctgag
     3361 ccgcgggatg acgtagtcgt ccagttgctg caacaactcc agccggacct tgccggccgc
     3421 cggggcatcc gggagcgcga gcggcagacg cgaggcctcc acctggtcgc gcagcccgtt
     3481 gaccgcggcg agcagcattg acagctcacc gggcgctggg ctcgctccgg tcacaggcgc
     3541 atccttccaa gtccatcggc ccgtctcaac acatgcagcc ccgacacgcc ccgggccggg
     3601 tgtgtcgcac gttcgcacag aggttcatgt cccctgcgtt cgagcccctg ttgccttctg
     3661 ccgtgggcgc tggcgcgccc ccggcaggac tcgaacctgc aacctgcgga ttagaaggcc
     3721 gctgcactat ccgttgtgct acgggggcag ttgggccgac ggagcagccg acggtccggc
     3781 gccccagtct atgtggcgtg ttccccccgc gccgcgacga acctccgaag ccggccggac
     3841 ggcatacccc gggcacaagg cgcccaccca cgcactcagc ggtcgcgggg gctgacctgg
     3901 ccgacgacga ccccgcggtt ggccgtcacg gcgtccaaca gatcgcgctc atgggcaccg
     3961 gcgccgggct cggctgaacc ccgcacgatc cgcgcccgca gcagggcgag gtcactggca
     4021 ctgtcctgga aggcgcgcat cgaccgcacg gccgcacgtc cccccaaacc gcgggcccac
     4081 gcgcgcgccg accggcgttg ccccatcgag gacaacatct cgacctcagc tctggtgaac
     4141 caaccagcat cggcgtactg cgacaggtag cgccgcacca accccgcttc gcgctggcgc
     4201 agcaggacca acccgacaag gaagacggcg aagatcggca tctggaaggc gaggtagacc
     4261 tgcaggtagg cgcccaccga cgcactgagg ttccagatcg cgtgcagggc catcgcgacc
     4321 accaacccca cggtcccgta ggcgatccga gcggccgcgg atcgcgccac cgtcgcggcc
     4381 aggccgatcc cgatccccgt gcacgccgtg aacagcgggt gcgcgaacgg ccccatgagg
     4441 caccgcagga agaacacctc ggtcagacct gacgtccccg cctcggcgaa ggcacgcccc
     4501 agatagagga tgttctcgct gaacgcgaac cccgctccgg ccatcccggc atagacgatg
     4561 ccgtcgatca ccccgtcgaa ctcgcgtcgc cgtcgccacg caatgaagag gatcgcggcg
     4621 cccttgagcg tctcttcgac caacggggcg ctgacgacgg cagtcaccgc gtcggcgttg
     4681 tcggtgtggg ccgcaaggaa gatcaggtgg atcccggtgt tgaggatgat cgcgccgacg
     4741 gccgcgcaca gcgctcccca gaggaaggcg aagatcaggt agcggaccgg ctcggcctcc
     4801 atccggtcga cccagaggaa gaccggcacg accaccagga gcggaaccgc ggagaccgca
     4861 gccgccacca gagagttgcc gatcccgatg tcgatcccga agaacaccga caacacggcg
     4921 agcgagatga ggaagaccga gatgatcacg ccggtcgtca cccagccgcg ccgttccggg
     4981 cgcctcgtcg ggttggggac gcctggaccc ggcatcgggt atgccgccgt ccccgagtcc
     5041 cagcccggca ccccagatcc ccccgtcgac atggccgaca gactagtcca gcgcagcgca
     5101 gcccccgggg cctctaacct ggtgccgtga gcatgagcac caacgcgtcg accaccccca
     5161 cctcctcgcc gaccgccggt tcctccggcg gcgaccgagc cctcgccgat cggatcgtct
     5221 ggatcgactg cgagatgacc ggcctgtcac tgcaggccga cgcgctggtc gaggtcgccg
     5281 cgctggtcac cgactacgag ctcaccgtgc tgggcgaagg cgtcgacgtg gtcatctgtc
     5341 ccccgccgga gtcggtggcc cagatggatc ctttcgtcgt cgacatgcac acgacctcgg
     5401 gcctgctcac cgaactcccc acgggcgtct cgcttcgcga ggccgaggag cgggtgctcg
     5461 cctacgtccg ggaatgggtg cctgaacccc gcaaggcggt gttggcgggg aacaccgtcg
     5521 gcacggaccg caccttcctc gcccgggaca tgcccgagct ggaggggtgg ctgcactacc
     5581 gggtcatcga tgtctcgtcg atcaaggaac tatcccgtcg ctggtacccc cgggcgtatc
     5641 acaactcccc ctcgaagtct ggggggcacc gagccctggc cgacatccgc gagtcgatcg
     5701 cggagttgcg ctactaccgt gaggcggtct tcgtgcccca gccggggccg gactccgcaa
     5761 cggcgcgggc tctcggcgag cggcacgtcg tcgttcaccc ggcggtcaac agcactgttg
     5821 ccgacccggt acgataggcg gctgtgcgtg gctttcgggt catgtccatg gtgggtgtag
     5881 ctcagctggt agagcaccgg gttgtggtcc cgggggtcgc gggttcaagc cccgtcactc
     5941 accccatgcg tttgagcgtc gcgcgtttcg agcgtcacga cgttcgaacg actgttcagg
     6001 tcgacatcgt tcgtcgaagg ggcgacccga tggtggttgg acggttcctg gccgtcgcgg
     6061 tggcagggct tgcctatgac gggatcgccg ccatcggcgc
//
LOCUS       DAOMZY010000220         6088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_12175_length_6088_cov_6.047074, whole genome shotgun sequence.
ACCESSION   DAOMZY010000220 DAOMZY010000000
VERSION     DAOMZY010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12175_length_6088_cov_6.047074"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="PLK64_11905"
     CDS             complement(<1..1119)
                     /locus_tag="PLK64_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPV80496.1"
                     /translation="MARAVGIDLGTTNSAVAVLEGGEPTIIANAEGGRTTPSVVAFSK
                     SGDVLVGEIAKRQAVTNVDRTIRSVKRHMGTDWKSDDIDGKRYTAQEISARILQKLKR
                     DAEAYLGEAVTDAVITVPAYFDDHERQATKEAGEIAGLNVLRIINEPTAAALAYGLDK
                     GKEDELILVFDLGGGTFDVSLLEVGKDPEDGFSTIQVKATSGDNRLGGDDWDDRIVTH
                     LLSTVKNSTGVDLSKDKIAMQRLRDAAEQAKKELSSSTSATINLQYLSMSENGPIHLD
                     ETISRAQFEQLTKDLLDRCKAPFAKVIKDAGIKLADIDHVVLVGGSTRMPAVTALVKE
                     LTGREPNKGVNPDEVVAIGASLQAGVLKGERKDVLLIDV"
     gene            complement(1424..1939)
                     /locus_tag="PLK64_11910"
     CDS             complement(1424..1939)
                     /locus_tag="PLK64_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HPV80497.1"
                     /translation="MERSGPSLRRVYGVKRWLYRGDRPNAVARTLNRFWSARFGSGGR
                     LARTRDVELIVTGRSSGRSLAFPIVLADINDTWYAVSMLGANANWVRNVRVAGGAATI
                     RHGQSWDVHLAEVPVPQRAPILKRYLDVAPGARPHVPVDRSEPVEAFVPIAADFPVFR
                     VDGFAPEAPRT"
     gene            complement(1978..2184)
                     /locus_tag="PLK64_11915"
     CDS             complement(1978..2184)
                     /locus_tag="PLK64_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80498.1"
                     /translation="MSYPKVTSVVAGIDQIEAVAPGDPQHPDSAGRVGGQLLAGDLAE
                     HVELPDVAVPDAYRVAGLDSLARR"
     gene            2433..3566
                     /locus_tag="PLK64_11920"
     CDS             2433..3566
                     /locus_tag="PLK64_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80499.1"
                     /translation="MLRGTHLAAHASVRGAPALIGIFLIEMLWLMPDLLETPYVLGTG
                     VSAITLVFTWVGANLVRRRRPFAAVARFGRIEALAFVLVPTAMVLATSHSAMDVEDLA
                     LSAGDVRLLLGITVFGAQLLLLAAVFALVVTGFVSLLSLLGRELIAALSRASGVLTAV
                     IPIMLGFVFFFFFNPGVWLGIANLDTFSFVALVVFLLALGGGFSGSRSQVDLDAHAAF
                     ETTEELTAALVGTPLPDGAAGIPTPAVCPLDSTQLASLRMVAVLSRLVTAAVLGSAVF
                     GIFLVMGFIVIGADTTLAWARTPPSIVFGIDLGGGIEHMVTWQQLRVAGFLAAFSSFN
                     YMLVAATDGRLRQDAADATTAIVRQACALRLAVLGQAPTQPTS"
     gene            3640..4245
                     /locus_tag="PLK64_11925"
     CDS             3640..4245
                     /locus_tag="PLK64_11925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPV80500.1"
                     /translation="MPSALVVIGSPNPRSFCHAMAMRAVAALERNGAECDIHDLNVEG
                     FQPVAPLDEASTVGMDVEAAIDAGSDGVVRTHRRALTQADYLVVTHPNWWGKPPAIMA
                     GWLDRVLVPGVAYRLEDRVGLPTSLLRLRSVLVLNTGDTPPERERQVFGDPLDDIWRR
                     CVGAYLGGAAVHRVVASPLGGSTEAEREAWLDEVGRVADAL"
     assembly_gap    4424..4523
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4524..5644)
                     /locus_tag="PLK64_11930"
     CDS             complement(<4524..5644)
                     /locus_tag="PLK64_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPV80501.1"
                     /translation="MSTSTHLRVDGLSFSYAGSPTSRPLLTDVTFTVSAGERVGLIGE
                     NGSGKSTLLRCLAGLLAPTGGAVTATTRGGSATLGLLHQLPPFDPDDTIADAVESAVA
                     PVREAERSLSLAADALAAAPAAPDARSAAAAYAQALAVAESFGVWEVDARIATTLAGL
                     GLGRLDRGRPTGTLSGGQQTRLSLAWLLLRRPDVLLLDEPTNHLDDAAVAFLLETLRT
                     WAGPVLFASHDRSFLDEAATGLLDLDPTAGRGPAAFTGAYTAYLSQRRETRERWERRF
                     RDEQEELRRLRQAVRDSHVVGHPGAAPRSEGRGAKKFYADRNARVVSRRVSDARSRLA
                     ELERAQVRKPPAPLSFAGLTPGYAGSASERSGPVLVASGV"
BASE COUNT          883 a         2020 c         2070 g         1015 t
ORIGIN      
        1 gacgtcgatg agcaggacgt ccttgcgctc acccttgagg acaccggcct gcaggctggc
       61 gccgatggcg acgacctcgt ccgggttgac gcccttgttg ggctcccggc cggtgagctc
      121 cttgacgagc gcggtcacgg cgggcatgcg ggtcgagcca ccgacgagga ccacgtggtc
      181 gatgtcggcg agcttgatgc cggcgtcctt gatgaccttg gcgaacgggg ccttgcagcg
      241 gtcgagcagg tccttggtga gctgctcgaa ctgcgcgcgg ctgatcgtct cgtcgaggtg
      301 gatcgggccg ttctcgctca tcgagaggta ctgcaggttg atcgtcgccg aggtggagct
      361 ggacagctcc ttcttggcct gttcggccgc gtcacgcagc cgctgcatgg cgatcttgtc
      421 cttggacagg tcgacaccgg tgctgttctt cacggtcgag agcaggtggg tgacgatgcg
      481 gtcgtcccag tcgtcgccgc cgagtcggtt gtcgccgctc gtggccttga cctggatcgt
      541 gctgaagccg tcctccgggt ccttgcccac ctcgaggagg gacacgtcga acgtgccacc
      601 accgaggtcg aagacgagga tgagctcgtc ctccttgccc ttgtccagac cgtaggcgag
      661 cgcggccgca gtcggctcgt tgatgatgcg caggacgttg aggcccgcga tctcgccggc
      721 ctccttggtg gcctggcgct cgtggtcgtc gaagtaggcg gggacggtga tgacggcgtc
      781 ggtcacggcc tcgccgaggt aggcctccgc gtcgcgcttg agcttctgca ggatgcgggc
      841 gctgatctcc tgcgcggtgt aacgcttgcc gtcgatgtca tcggacttcc agtccgtgcc
      901 catgtgacgc ttgaccgacc ggatcgtccg gtcgacgttg gtgaccgcct gtcgcttggc
      961 gatctcgccg acgaggacgt caccgctctt gctgaaggcg acgaccgagg gggtcgtccg
     1021 gccgccctcc gcgttggcga tgatggtcgg ttcgccgccc tcgagaacgg cgacggccga
     1081 gttggtggta ccgaggtcga tgccgaccgc acgtgccatg tttgttgtct cctgtgttgc
     1141 tgggcggcat accaaggggt atgccgggtg ggtgagtctt cgacgctgaa tctgctccgc
     1201 gggggctggg ttgtcaaccc caggctcggc aaagttgcgc ttactaggct caactcttgg
     1261 tatgccgccc ttgttcccgc ctgggtgggg ctggtttcgc gaccctcgga cccccggatg
     1321 ccgcggaacc ctcgccactg tgaggaagtg gggccgcaac ccgccttcgg gccccgaatt
     1381 cgccggcgtg tgtggggaat tcgggtcagg ggtggcagcg aagttaggtg cgcggggcct
     1441 ctggggcgaa gccgtcgacg cggaagacgg ggaagtccgc agcgatgggg acgaaggcct
     1501 caacgggctc cgagcggtcg acggggacat gcggtcgggc tccaggagcc acgtcgagat
     1561 aacgcttgag gatcggggcc cgctgcggca cagggacttc cgcgaggtgc acgtcccacg
     1621 attggccgtg ccggatggtc gccgcgccac cagccacgcg cacgttgcgg acccagttgg
     1681 cgttcgcgcc gagcattgac acggcatacc aggtgtcgtt gatgtcggcg aggacgatcg
     1741 ggaaggcgag agatcttccg ctcgacctgc cggtgacgat caactcgaca tcgcgggtgc
     1801 gggccaggcg ccctccggag ccgaaacgag ccgaccagaa ccggttgagc gtcctggcca
     1861 cggcgttcgg gcgatcaccg cggtaaagcc accgcttgac accgtagacg cgacgcagtg
     1921 agggcccaga tctctccacc gtccgagcgt ctcacgcgcg aaggccccga cccccagtta
     1981 acggcgcgcg agtgagtcca ggcctgccac gcgataggca tcgggcaccg cgacgtcggg
     2041 caactccacg tgctccgcga gatcacccgc gagcaactgg ccgcctaccc gtcccgcgct
     2101 gtcggggtgc tgggggtcgc cgggggcgac ggcctcgatc tgatcgatcc ccgcgaccac
     2161 ggacgtcacc tttggatacg acatcaaccc ggactatgtg cacgcctgcg acaccgggta
     2221 tcgcgacggc ctcggcgagc gcctgcacct catcgagtca gcatcgagcg cacggccacg
     2281 attcgccaag acttccgcgt cgcagcctgc tgagcccggg ccgagctggc tgcaccctcc
     2341 gggaacaccc cttaggattc gccgtcgtga agttcctccg gcctgcaccg ccgacaccgg
     2401 agcggtggtt cctgcggcat ggtctcgttt ccatgctccg gggcacgcac ctggccgccc
     2461 atgccagcgt gcgtggagcg cccgcgctca tcgggatctt cctgatcgag atgctgtggc
     2521 tgatgccaga cctgctcgag actccctacg tactggggac gggagtgtcc gccatcacgc
     2581 tggttttcac gtgggttggc gccaacctcg tgcgccgccg ccgaccgttc gccgcggtcg
     2641 cccgatttgg acgaatcgaa gccctggctt tcgtgctggt gcccacagcc atggtccttg
     2701 cgacgtcgca ttcggccatg gacgtggagg acttggcgct cagcgccgga gatgtgcgac
     2761 tgctgcttgg catcaccgta tttggcgcgc agctgctgtt gctcgccgcg gtgttcgcgc
     2821 tcgtcgtcac cggattcgtg tcgttgctca gcctccttgg ccgtgagctc atcgctgcac
     2881 tctcgcgcgc gagcggcgtc ctgaccgcag tcattccgat catgttgggc ttcgtcttct
     2941 tcttcttctt caacccgggc gtctggttgg gcatcgcgaa cctcgacacc ttctccttcg
     3001 tcgcattggt cgtcttcctc ctcgccctcg gcggcggctt cagcggcagc cgaagccagg
     3061 tcgatctcga cgcacacgcg gcgttcgaga cgaccgaaga actcacggca gccttggtag
     3121 gaacgccgtt gcccgatgga gcagccggga tcccgactcc cgcggtctgc cccctcgact
     3181 cgacccaact ggcgagcctg cggatggttg cggtgctctc ccgactcgtt accgccgccg
     3241 tcctcgggtc ggccgtcttc gggatcttcc ttgtcatggg cttcatcgtc atcggcgccg
     3301 acaccacgct ggcctgggcc aggaccccgc cgtcaatcgt gtttggcatc gatctgggcg
     3361 gcggcatcga gcacatggtc acctggcagc aactccgggt agccgggttc ctcgctgcgt
     3421 tctctagctt caactacatg ctggtggcgg cgaccgacgg gcggctacgc caggatgccg
     3481 ccgacgcgac caccgccatc gtccgacaag cgtgtgccct gcggctggcc gttctcggcc
     3541 aggcgccaac ccaaccgacc tcctgacccg gcaacacggc atacccgctg acgcgaggag
     3601 atccgccggg gtggccgcgc ggagtgggac gctggacgcg tgccttcagc cctggtcgtg
     3661 atcggcagcc ccaacccgcg cagcttctgt cacgccatgg ccatgcgggc ggtcgccgcg
     3721 cttgaacgga acggcgcgga gtgcgacatc cacgacctga acgtcgaagg attccaaccg
     3781 gtggcgccgc tcgatgaagc ctcgaccgtg gggatggacg tcgaggcggc catcgatgcc
     3841 ggcagcgatg gtgtggtgag aacgcaccgt cgggcgttga cccaggccga ctatctcgtt
     3901 gtcactcacc cgaactggtg gggcaaaccg ccagcgatca tggccggctg gctcgaccgg
     3961 gtgctggtcc ccggggtggc ctatcgactg gaggaccgcg taggcctgcc gacctcgttg
     4021 ctgaggttgc gctctgtctt ggtcctcaac accggggaca cgccaccgga acgcgagagg
     4081 caggtgttcg gagatccctt ggacgacatc tggcggcgct gcgtcggcgc ctacctgggg
     4141 ggcgcggccg tccatcgggt cgtggcgagt cctctcggcg gatcgactga ggcggagcgt
     4201 gaggcctggc ttgacgaggt cggccgggtg gctgacgcct tgtgaatctc gggcgggcaa
     4261 gtcaagccga gcccaactcg agccgccgtc cggcccaacg tgagcgcagc caacgatcat
     4321 gactggccac gacgaccgcc cccgggtaat caggcagcga ctcctcgatg gcgtcgacca
     4381 gcggcagcga cagatggttc gtcggctcgt cgagcagcaa cacnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnaccccgg acgccacgag gaccggaccc gaccgctcac
     4561 tggcggaccc ggcatacccc ggggtcaggc cggcgaagct cagtggcgcg ggcggcttgc
     4621 gcacctgagc gcgctccaac tcggcgagcc gggaccgcgc gtcactgacc cggcgcgaga
     4681 cgacacgcgc gttccggtcg gcgtagaact tcttcgcgcc gcggccctcg ctgcgtggcg
     4741 cggcgcccgg gtgcccgacg acgtgcgagt cgcgcaccgc ctgccgcagc cgacggagct
     4801 cctcctgctc gtcgcggaat cgtcgctccc agcgctcccg ggtctcgcga cgctggctga
     4861 ggtatgccgt gtacgcgccg gtgaacgccg ccggcccgcg ccccgcggtc gggtcgaggt
     4921 cgagcaaacc ggtcgctgcc tcgtcgagga aggaccggtc gtgactggcg aagagcaccg
     4981 ggccggccca ggtccgcagg gtctcgagca ggaacgcgac ggcggcgtcg tcgaggtggt
     5041 tcgtcggctc gtcgagcagc agcacatccg ggcgacggag cagcagccac gcgagtgaca
     5101 gccgggtttg ttgacctccc gagagcgtgc cggtggggcg acctcgatcg agtcggccta
     5161 gcccgaggcc cgcgagcgtc gtcgcgatcc gagcgtcgac ttcccacacg ccgaagctct
     5221 cggcaacggc caacgcctgg gcgtatgccg ccgcggccga ccgtgcgtca ggcgcggcgg
     5281 gcgccgccgc gagggcgtcg gctgccaggc tgagcgatct ctcggcctcg cgcaccgggg
     5341 ccactgccga ctccacggca tcagcgatgg tgtcgtcggg gtcgaagggc ggcagttggt
     5401 gcagaagccc gagggtggcg ctgcccccgc gcgtcgtcgc cgtgaccgcg cctccggtcg
     5461 gtgccagcag gcctgccaga caacgcaata gcgtcgactt gcccgagccg ttctcgccga
     5521 tgaggccgac ccgctcgccg gccgagacgg tgaaggtgac atcggtgagc agcgggcggg
     5581 aggtgggcga accggcatac gagaaggaca gtccgtccac gcgaaggtgg gtcgaggtgc
     5641 tcatgggtcc tcgtcggagg tcgggacatc cccggcgggc acgacggccg cagcgcgggg
     5701 cggatcacga ctcaactgag cacgaggccg acgctacccg gatcgggcag agggtcgcca
     5761 gcgagttttc cgttgccgcg cggctaacct gatcacccgt tgggctcgca caccccatgg
     5821 acactgagaa cccttgtgac tgtcgggaat tcgggccccc aagggcctgc gaccatcgat
     5881 atcaccggcg agtgtgatga attcggggca gccaccaccg gcgcatatcg tggggtcacc
     5941 cgaccgattg ccttcgacgt gaggtcgtca tgacactccc cacgaacccg ctgatcctgc
     6001 acgggtgccc catccatccg ggcggcggcc gagctcccgt cgaagccatc gccatcgacg
     6061 gcgaccgcat cgtggcgacc ggcagcct
//
LOCUS       DAOMZY010000221         6049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_12290_length_6049_cov_5.797297, whole genome shotgun sequence.
ACCESSION   DAOMZY010000221 DAOMZY010000000
VERSION     DAOMZY010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12290_length_6049_cov_5.797297"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="PLK64_11935"
     CDS             complement(<1..719)
                     /locus_tag="PLK64_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004005544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HPV80502.1"
                     /translation="MDLKLTTKAQEALSAAVRASAAAGNPHTEPAHLLKALTEQSDTT
                     TPALLEAAGSSLQAASRVAEQALSGMPSVSGSSVSAPGLSRSAMAVLEAARAAMAAMG
                     DTYVSTDHLLLALAKTGRFGLDARAIETAIPTLRGGRSVDSENPEGTFDALTKYGTDL
                     TARAREGKLDPVIGRDAEIRRVVQVLSRRTKNNPVLIGEPGVGKTAVVEGLAQRIVDG
                     DVPESLRGKKLISLDLGAMVAG"
     gene            846..1211
                     /locus_tag="PLK64_11940"
     CDS             846..1211
                     /locus_tag="PLK64_11940"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV80503.1"
                     /translation="MTFWTASEGDAVVGCAALRELAPDEAELKSMRTVPSARGRGVGT
                     ALLRQVLDEARARGYRRVSLETGTHAFFTPAHQLYAAHGFLPCAPFGTYVEDPHSRFF
                     TLDLSDASAAGNTSNASSP"
     gene            complement(1216..1791)
                     /locus_tag="PLK64_11945"
     CDS             complement(1216..1791)
                     /locus_tag="PLK64_11945"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPV80504.1"
                     /translation="MERTGRQNELADAGLRLVAGEGMGGVTFRAVAAESGWSLGAVQR
                     AFGSKEDLQAAMLERLRTSVEPQVPGEPGRPTLDVWLVELVIAVAPFDDRRRAMQRQG
                     HAFAELAPFDDRIGQVIAEDDDRLRALLARLVRRAQSEGEIESSVDPEGVAWSVLALM
                     QGVASQLLYDMQPEADVRRWVDAVVQGVLHL"
     gene            1938..2471
                     /locus_tag="PLK64_11950"
     CDS             1938..2471
                     /locus_tag="PLK64_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HPV80505.1"
                     /translation="MTSRIEAIRADLTRESLDAIVNAANATLLGGGGVDGAIHAAAGP
                     RLLEACRGVRRTAYPQGLPVGYAVATGAGNLPCRWVIHTVGPNWNRGQRDEADLAACF
                     TNSLREAVRVGARSVAFPAVSAGAYGWAGPTVARVAVAAVRSAPELADLDLVRFVLFS
                     DRLLADFRAALNDEARA"
     gene            complement(2490..2738)
                     /locus_tag="PLK64_11955"
     CDS             complement(2490..2738)
                     /locus_tag="PLK64_11955"
                     /inference="COORDINATES: protein motif:HMM:NF017747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HPV80506.1"
                     /translation="MTIDGTTVDLTAGEQVRFPGEAVVAVVLSRPTRALNVMTRRGRC
                     VAQVALRPADAPARGEGTVSVDLGERIAEVRVTIAPRL"
     gene            complement(2735..2929)
                     /locus_tag="PLK64_11960"
     CDS             complement(2735..2929)
                     /locus_tag="PLK64_11960"
                     /inference="COORDINATES: protein motif:HMM:NF017747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HPV80507.1"
                     /translation="MLDPAAFDPVPWANGAGTTRELALARDAEGQTLWRVSLADLDQS
                     ASFSSVPGLDRTSPLSGRSA"
     gene            complement(2955..3224)
                     /locus_tag="PLK64_11965"
     CDS             complement(2955..3224)
                     /locus_tag="PLK64_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019484296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HPV80508.1"
                     /translation="MELTPDELAKLANRLRRAQGQIGGVLRMIEEGRDCQDIVTQLAA
                     VRRAVDRVGFSIIATGLKQCVTEAHDRGDNEVDTALMEKLFISLA"
     gene            3390..4637
                     /locus_tag="PLK64_11970"
     CDS             3390..4637
                     /locus_tag="PLK64_11970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HPV80509.1"
                     /translation="MRTLVILGGGTAGTMAANKLRARLAAEEWQIIVIDKDDNHDYQP
                     AYLFVPFGINKVKQVRKSRRSFIDDGIEVRMADIERVDAEARLVHLGDGSELGYDYLI
                     MATGTTPRPDQTPGMLGPEWRKSVHEFYTYDGMLALKKSLKDFQGGRLVMHITELPIK
                     CPVAPLEFVMLADDFLTKKGVRAATEIVYVTPLDGAFTKPVASRELGHHLSTRNIKVE
                     PDFMIESVDQEAKVIRSYDEREVAYDLLVTVPLNMGADYVAASGLGDELNYVPVDKHT
                     NQSTAYPNIFALGDGSNLPTSKAGSVAHFSVEVFVDNFCELIEGRPMTHSFDGHANCF
                     VESGNGRALLLDFNYETEPLTGHFPLPVGPMSLLKATRANHVGKLAFRHVYWNVLLPG
                     RPLPLHAEMSMLGKRVEPQPVDA"
     gene            4716..5030
                     /locus_tag="PLK64_11975"
     CDS             4716..5030
                     /locus_tag="PLK64_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HPV80510.1"
                     /translation="MPTTTIAGRQIDVDAEGFLTQPDQWDEDLALTLAANIGITLTDA
                     HWVPIHFLRKDFETQKETATLRRVSTVGGIPVKELFVLFPGKPAKKMSYIAGLQKPKG
                     CV"
     gene            5074..5598
                     /locus_tag="PLK64_11980"
     CDS             5074..5598
                     /locus_tag="PLK64_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HPV80511.1"
                     /translation="MTDMIVPSFDDETPTGRKLAIICSKGNLDMAYPGLILANAALGE
                     GIEVHLFFTFWGFDIINKETMGNLKFSYLGNTAMHMPGHFGMSMPQGMAGLPGVTAMT
                     TKMMKKQISDLDVPEVPEFLELLHGSGAKMWACRMSADMMHLELEHLYDGVEAIISAT
                     DFIELTDGAQMLFI"
     gene            5747..>6049
                     /locus_tag="PLK64_11985"
     CDS             5747..>6049
                     /locus_tag="PLK64_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80512.1"
                     /translation="MEAPVSTLAATEVVALVGTTEASPAAAALAVIEVVERMKSHLDA
                     LVLPAWRMLHAAYVEALESPDRPPMTARERERETSVARSGAVDEVMAATGLGESECR"
BASE COUNT         1014 a         2120 c         1957 g          958 t
ORIGIN      
        1 cccgcgacca tcgccccaag gtcgaggctg atgagcttct tgccgcgcag gctctcgggc
       61 acgtcgccgt cgacgatccg ctgggccagg ccctcgacca cggcggtctt gccgacaccg
      121 ggctcgccga tgaggacggg gttgttcttc gtccgccggc tgagcacctg gacgacccgc
      181 cggatctcgg cgtcacggcc gatgaccggg tcgagcttgc cctcgcgggc ccgcgcggtg
      241 aggtcggtgc cgtatttcgt cagggcatcg aaggtcccct cggggttctc cgagtcgacg
      301 ctccggccgc cgcgcaacgt cggaatggcg gtctcgatgg cgcgggcgtc cagcccgaag
      361 cgacccgtct tggccagcgc cagcaacagg tgatcggtcg agacataggt gtcgcccatg
      421 gcggccatcg cagcccgagc ggcttccaga acggccatcg ccgatcggga gagccccggt
      481 gcgctgaccg acgaaccgga gacggacggc ataccggaca acgcctgctc ggccacgcgg
      541 gaagcggcct ggaggctgga accggctgcc tcgagcagcg cgggcgtcgt cgtgtcggac
      601 tgctcggtca gtgccttgag caggtgggcg ggctcggtgt gggggttgcc ggcagcggct
      661 gacgcgcgga cggcagccga cagcgcctcc tgggccttgg tcgtcagctt gagatccatg
      721 ggtatgtcgt gtgctcctta tgaggggatg ggccttctct agggcaactc cgtcaaagtt
      781 gagtctattc cgctcaacct cggccaaggg ggttccgtcc cctagggttt ggggcccggc
      841 ccgacatcac cttctggacc gcgagcgagg gcgatgcagt cgtgggttgc gccgcactgc
      901 gcgagctcgc gccggacgag gcggagctca agtcgatgcg cacggtcccc agcgcgcgcg
      961 ggcgaggcgt cggcaccgcc ctcctacgcc aggtcctcga cgaagcgcgc gcccgcggct
     1021 accgcagggt cagcctggag accggcaccc acgcgttctt cacgcccgcg catcagctgt
     1081 atgccgcgca cggtttcctt ccctgcgcgc cgttcgggac ctacgtcgag gacccgcatt
     1141 cgcggttctt cactctcgac ctcagcgacg cgagcgccgc gggcaacacg agcaacgcat
     1201 cgagcccctg acgcgtcaga ggtgcagcac tccctgcacc acagcatcca cccaacgccg
     1261 gacgtcggcc tcgggctgca tgtcgtagag caactggctg gcgacccctt gcatgagggc
     1321 caggaccgac cacgcgacgc cctcgggatc gacgctgctc tcgatctccc cctcgctctg
     1381 ggcgcgacgc accagccgag cgagcagagc ccgcagccga tcgtcgtctt cggcgatgac
     1441 ctgaccgatc cggtcgtcga agggggccaa ctcggcgaag gcgtgccctt ggcgctgcat
     1501 cgcgcgtcgc cggtcgtcga agggggcgac cgcgatgacg agttcgacca accagacgtc
     1561 cagcgtggga cggccgggtt cgcccgggac ctgcggctcc accgacgtgc gcagccgctc
     1621 caacatcgcc gcctgaaggt cttccttgct gccaaaggcc cgttggacgg cacccagcga
     1681 ccatcccgac tcggctgcaa cggcccggaa ggtcacgccg cccatgccct cacctgcgac
     1741 cagacgcagc cccgcgtcgg ccaattcgtt ctgtcgacca gtccgctcca cggggtcact
     1801 ctatggtcgc aaatcgctca ttgttgccta aaagatctga tctctaccgt ctcccggcat
     1861 atctgtctaa acggacgatg gccacgatca cctctaccgt tgggcgcagg ccgctatcga
     1921 cacccgagga ggaagccgtg acgtcccgga ttgaggcgat ccgcgcagac ctcacccgcg
     1981 agagcctgga tgccatcgtc aacgccgcca acgcgaccct gctcgggggc ggcggggtcg
     2041 acggggcgat ccacgccgcg gccggcccgc gcctgctgga ggcgtgccgc ggggtgaggc
     2101 gcacggcata cccgcaggga ctccccgtgg ggtatgccgt ggccaccggc gccgggaacc
     2161 tcccctgccg ctgggtgatc cacacggtcg gccccaactg gaaccgcggc caacgcgacg
     2221 aagccgacct ggcggcctgc ttcacgaact cgctgcgcga ggcggtccgg gtcggcgccc
     2281 gctcggtggc cttccctgcg gtcagcgccg gcgcctacgg gtgggccgga ccgacggtgg
     2341 cgagggtggc cgtggctgcc gtgcgctcgg caccggagtt ggctgacctc gacctcgtgc
     2401 gcttcgtcct gttcagcgac cgcctgctgg ccgacttccg cgccgccctg aacgatgagg
     2461 ctcgcgctta gtcggtcagg cggaggcggt cagagtcgag gggcgatggt gacgcggacc
     2521 tcggcgatgc gctcgccgag gtcgacgctc accgtcccct cgccacgtgc cggagcgtcg
     2581 gccggccgga gggcgacctg ggcgacacac ctgccgcggc gcgtcatcac gttaagggct
     2641 cgggtcgggc gagacagcac gaccgcgacc acggcttcac cggggaaccg aacctgctcg
     2701 ccggcggtta ggtcgacggt ggtgccgtcg atcgtcaggc ggaccgaccc gagaggggtg
     2761 aagtccggtc gaggcccggc accgaggaga acgacgcgct ctgatcgagg tccgcgaggc
     2821 tgacccgcca cagggtttgg ccctcggcgt cgcgagcaag ggccagctcc cgagtggtcc
     2881 cggcaccgtt ggcccaggga acagggtcga acgccgccgg atccagcatc accgcaggct
     2941 ccgcgtcgtc acggctaggc gagggagatg aagagcttct ccatgagggc ggtgtcgacc
     3001 tcgttgtcgc ccctatcgtg ggcctcggtc acacactgct tgaggccggt ggcgatgatc
     3061 gagaagccga cccggtccac cgctcggcgg accgcggcca gctgcgtgac gatgtcctgg
     3121 cagtcgcgac cctcttcgat catccgcagc acacccccga tctgaccctg agcgcggcgc
     3181 agccggttgg ccagcttcgc caactcgtca ggcgtcagtt ccacctttgt catacccgtc
     3241 atcctcccac gactccttcg gctgatcggg gggttcgctt tgctgcacgg cacacagggg
     3301 aggaccttcg gccccgctgg acagcatacc cgcgggggta ttctctcatg tatcaggtta
     3361 ctccgcgaga actacataag gacccagcaa tgcgaaccct cgtgatcctc ggcgggggaa
     3421 ccgccggaac catggcagcc aacaagctgc gggctcgact ggcggccgag gaatggcaga
     3481 tcatcgtcat cgacaaggat gacaaccacg actaccagcc ggcctacctg ttcgtgccgt
     3541 tcggcatcaa caaggtcaag caggtgcgca agtcccgccg gtcgttcatc gatgacggca
     3601 tcgaggtgcg gatggccgac atcgagcgcg tggacgccga agcccgactc gtccacctcg
     3661 gtgacggcag cgagctcggt tacgactacc tcatcatggc cacggggacg accccccggc
     3721 cggatcagac tcccgggatg ctcggccccg agtggcgcaa gagcgtccac gagttctaca
     3781 cgtacgacgg catgttggcg ctgaagaagt cactcaagga cttccagggc ggccgactgg
     3841 tcatgcacat caccgagctg cccatcaagt gccccgtcgc cccgctcgag ttcgtcatgc
     3901 tggccgacga cttcctcacc aagaagggcg tgcgggctgc caccgagatc gtctacgtca
     3961 ccccgctgga cggcgccttc accaagccgg tcgccagccg cgagctcggc catcacctgt
     4021 ccacgcgcaa catcaaggtc gagcccgact tcatgatcga gtcggtcgac caggaggcga
     4081 aggtcatccg ctcctatgac gagcgcgagg tggcctacga cctgctggtc accgtgccgc
     4141 tcaacatggg cgccgactat gtggctgcct ccggcctggg cgacgagctc aactacgtgc
     4201 ccgtcgacaa gcacaccaac cagtccacgg catacccgaa catcttcgcg ctcggggacg
     4261 ggagcaacct gcccacgtcg aaagccggct cggtggcgca cttctcggtc gaggtcttcg
     4321 tcgacaactt ctgcgagctc atcgagggcc gcccgatgac ccactccttc gacgggcatg
     4381 ccaactgctt cgtcgagtcc ggcaacggtc gggcgctgct gctggacttc aactacgaga
     4441 ccgagccgct caccggacac ttccccctgc cggtcggccc gatgagcctg ctcaaggcca
     4501 cccgcgccaa ccacgtaggc aagctggcct tccggcacgt ctactggaat gtcctgctcc
     4561 cgggccggcc gctgccgttg cacgcggaga tgtcgatgct cggcaagcgc gtcgagcccc
     4621 agcccgtcga cgcctgaccc accgccagac aatcccgcac cgactcccat ccacgaccat
     4681 ccccaccagc agaaacccaa ggagaaccga cccccatgcc caccaccacg atcgccggcc
     4741 gtcagatcga cgtcgacgcc gagggcttcc tcacccagcc ggatcagtgg gacgaggacc
     4801 tcgccctgac cctcgctgcc aacatcggca tcacgctgac cgacgcccac tgggtgccga
     4861 tccacttctt gcgcaaggac ttcgagaccc agaaggagac cgcgacgttg cgtcgcgtgt
     4921 cgaccgtggg cggcataccg gtcaaggaac tgttcgtgct cttccccggg aagcccgcca
     4981 agaagatgtc ctacatcgcc ggcctgcaga agcccaaggg ctgcgtctga tcgcccagat
     5041 cagccagttg actacgaaga gaagaagatc acgatgaccg acatgatcgt cccgtcgttc
     5101 gacgacgaga cgccgaccgg gcgcaagctc gccatcattt gctccaaggg caacctcgac
     5161 atggcctacc ccggcctcat cctcgccaac gccgccctcg gcgaaggcat cgaggtgcac
     5221 ctgttcttca ccttctgggg tttcgacatc atcaacaagg agacgatggg caacctgaag
     5281 ttctcctacc tcggcaacac ggcgatgcac atgcccggcc acttcggcat gtcgatgccg
     5341 cagggcatgg ctggcctgcc gggcgtcacc gccatgacga ccaagatgat gaagaagcag
     5401 atttccgatc tcgacgtgcc tgaggtgcct gagttcctcg agctcctgca cggctccggc
     5461 gccaagatgt gggcctgccg gatgagcgcc gacatgatgc acctcgagtt ggagcacctt
     5521 tacgacgggg tcgaggcgat catctcggcc accgacttca tcgagctcac cgacggggca
     5581 cagatgctgt tcatctgagg tcgtcccatg cgggaggtat gccgcgcgct caccgtgagc
     5641 gggcggcata cctgcgtctg ggttgggctg agtttggcgg caattcgcct gtcctgcaat
     5701 tcgattccct ttgtcatcga acgggtgttc gggtagaatc tagagcatgg aagcccccgt
     5761 cagcaccctc gccgcgactg aggtcgtggc gttggtcggc accacggagg cgtctccggc
     5821 agccgccgcg ctggcggtga tcgaggtcgt cgagcggatg aagtcgcatc tggacgccct
     5881 ggttctgccg gcctggcgaa tgctccacgc ggcatacgtc gaggcgttgg agtcgccgga
     5941 tcggccgccg atgaccgccc gcgagcgaga gcgcgagacg tcggtcgccc gatccggagc
     6001 ggtcgacgag gtcatggccg cgaccgggct cggcgagtcg gaatgccgt
//
LOCUS       DAOMZY010000222         6048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_12295_length_6048_cov_7.276322, whole genome shotgun sequence.
ACCESSION   DAOMZY010000222 DAOMZY010000000
VERSION     DAOMZY010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12295_length_6048_cov_7.276322"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..961)
                     /locus_tag="PLK64_11990"
     CDS             complement(176..961)
                     /locus_tag="PLK64_11990"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPV80513.1"
                     /translation="MSALLLAEFLGCRLGLLLALVMLLQGAPEKLPEPYAVATLNIHG
                     SVDRNGNGNAGKPEAVTRDVVALAKQYQPRVIALQELCLRQHRSIRPALAKLGYVATM
                     AYVDVSGGCNDKAKGNKAGIAVYLKTSKISWRKSAALPWGKNAVGTTGRQPRRVLCAQ
                     GKGWREIACTTHLAPSDPDRAAQAAKARSVIAGWAKKTPIVLAGDLNMSQAAASAAFP
                     GLGIAGYRIDWALANDRVALVAAAPVPSSDHPVVVVSVTPGSP"
     gene            complement(1020..1421)
                     /locus_tag="PLK64_11995"
     CDS             complement(1020..1421)
                     /locus_tag="PLK64_11995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPV80514.1"
                     /translation="MKPYYSDPFVTLYHGDCLEVTEWLAADVLGRWPKSTSMLSAVVS
                     TRALAAGGYSGYATRAGHPHAKPLDVMESLIEACPPGIIADPFAGSGSTLVASKALGR
                     RAIGVELEERYAEIAARRLAQDVLDFGGEAS"
     gene            complement(1418..1552)
                     /locus_tag="PLK64_12000"
     CDS             complement(1418..1552)
                     /locus_tag="PLK64_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80515.1"
                     /translation="MLDLWAHLRNIRDDGTVAWVLVHKILDLGWRPMGTVPAPEGIEP
                     "
     gene            complement(1726..2478)
                     /locus_tag="PLK64_12005"
     CDS             complement(1726..2478)
                     /locus_tag="PLK64_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80516.1"
                     /translation="MTMTREELQRLYDDCQDGRYGHLVALVDGVRRFLEGDDPTVEIA
                     YLKAKLADVEAERVRLRRGRESAGGSASPGGPVMSHDDIKAALAKVNPTGEQWCIWNG
                     WGPLVGTEDIHAATRIGTSDEDSGVHGRWTEDGDLYGSRDTLEFVASAPTWLAELLAE
                     VDRLSHWKAEALPVIDGLQELGAELGIRLGGRITGPDALAAVKALTARAEAAEQANQR
                     VRAQCDSWSHGNSWNKAARRAILRALDGGDPT"
     gene            complement(2475..3065)
                     /locus_tag="PLK64_12010"
     CDS             complement(2475..3065)
                     /locus_tag="PLK64_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80517.1"
                     /translation="MILTHRPLQLPWGQPVTEQRRSRWTFKAAWPATLSLLDRELGHL
                     DADAVILQVDVGEASVRLNGQLRADARPTFPGVRLIVDTPRGTLSWQTDVCEDWQHNV
                     RSIALGLEALRAVDRYGITTTAGEQYKGWLQIEAGRSAESPVEVLARIAGESPKLPEA
                     ALLRRARAAAHPDRNGGDRSLWDQVEAAATLLGLGA"
     gene            complement(3062..3526)
                     /locus_tag="PLK64_12015"
     CDS             complement(3062..3526)
                     /locus_tag="PLK64_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80518.1"
                     /translation="MTDLISRAVLRKAVLDHLHGIDREARDALLYTMRPGDRLTAWDD
                     ETDLGMVYVTKPKPTYRITDPAAFLAWVEANRPDEIVTTVAVSGNFTELIRRQGEAVD
                     PETGEVVVPDGVSAEPVPGSPTLTVKPSAAAVAIADQQIAALPQLTQGGRAA"
     gene            complement(3672..4316)
                     /locus_tag="PLK64_12020"
     CDS             complement(3672..4316)
                     /locus_tag="PLK64_12020"
                     /inference="COORDINATES: protein motif:HMM:NF016302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HPV80519.1"
                     /translation="MSGELAKALAAFQGEAPKVAKDKTAKVPTKSGGSYSYSYADLAD
                     IAAAAYPLLSKHGLSFTCLPEVDPPRMTLRGVLLHISGERIEGVLPLTGSTPQEIGSS
                     LTYARRYMLGCLTGIVTDADDDGALASKPRAQQRREAPRESEAWPETTPPADEPITDK
                     TRAQLYALMGERHVSKPEDQRAAMGKVLGRVVESRSTLTEVEGRKLIASLRGQG"
     gene            complement(4581..5033)
                     /locus_tag="PLK64_12025"
     CDS             complement(4581..5033)
                     /locus_tag="PLK64_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80520.1"
                     /translation="MSIVYLTLAISDHYPAGTCWAWMEKAEKACGRPEGAEPHLCPRH
                     EVVARRRLDQRIAREAARNVQRKTDSISKLPKLRAQLAKVEAEIERRDPKPPTDDPAA
                     FGGVGCTTTARWQKRVMSDSNISRMATLWDEQRRLAEAVRIAERWANS"
     gene            complement(5030..5182)
                     /locus_tag="PLK64_12030"
     CDS             complement(5030..5182)
                     /locus_tag="PLK64_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80521.1"
                     /translation="MSAEVRAHADREHRRKASRAARGWLGALIVGAVAWVGLIMLGVK
                     AAEWLQ"
     gene            complement(5188..>6048)
                     /locus_tag="PLK64_12035"
     CDS             complement(5188..>6048)
                     /locus_tag="PLK64_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80522.1"
                     /translation="SSRVVARESSRVVARGSSRVEAWESSRVEARESSRVEARGSSRV
                     VARESSRVVARGSSHVEARGSSHVEAWESSHVEARESSHVEAWESSRVEARESSHVEA
                     RESSHVEARGSSRVEARESSRVEAWGSSHVEAWESSRVVARESSHVEARESSSVEAGR
                     FTAVHLWSQRVTLTGEGHIIDMTAVDLTEVKTWHAYIGADSLVRPDRDQMVVGHVSSQ
                     DHEGRLGKDGQIVIGCFEGDVAKLRKLIEGEHWPSHADADRRAKFRPRILAFADLCER
                     QLAAWAEAAK"
BASE COUNT          921 a         2047 c         2079 g         1001 t
ORIGIN      
        1 gtcgcgggac agctcggcga tcctcggcca cgcacggccg gagacgaccg gcgcgctccg
       61 gccgggggct tggggttggc ggctgcgggc ctcggcgatg gctccgaggg ccaggagctc
      121 gccggctgag aaggggcagc gatggtcggt catgactgcc agatttcggg ggtcatcatg
      181 gacttcctgg ggtgacggag acgaccacca cgggatggtc gctggagggg acgggggcgg
      241 cggcaacgag ggcgacccga tcattcgcga gtgcccagtc gatccgatag ccggcgatgc
      301 cgagcccggg aaacgcagca cttgccgcgg cctgggacat gttcaggtcg ccggccagga
      361 cgatcggagt cttcttcgcc cagccggcaa tgacgcttcg ggccttagcg gcctgggctg
      421 cgcggtcggg atcggagggt gcgaggtggg tggtgcaggc gatttctcgc catcccttgc
      481 cctgagcgca gaggacccgg cgtggctggc gaccggtggt acccaccgca ttcttacccc
      541 acggcaatgc cgcagacttc cgccaggaga tcttcgaggt cttcagatag acggcgatgc
      601 cagccttgtt gcccttggcc ttgtcgttgc atccaccgga tacgtcgaca taggccatgg
      661 ttgccacgta gccgagcttg gcgagggctg gcctgattga gcgatgctgt cgcaggcaaa
      721 gctcctggag ggcgatgact cgcggctggt attgcttggc gagtgcgacg acatcgcggg
      781 tgaccgcttc gggcttgccg gcattgccgt tgccattgcg atccacgctg ccgtggatat
      841 ttagggtggc caccgcgtag ggctccggca gtttctccgg agccccttgc agtagcatca
      901 ccagggcaag caagagcccc aggcgacagc caaggaactc cgcaaggagt aacgcgctca
      961 cgatcactca ccgcctaggg cgagcagggt gccggcgtcg aaggggcacg gatggttggt
     1021 catgacgcct ccccgccgaa gtcgaggacg tcttgggcca accggcgggc cgcgatttcc
     1081 gcgtagcgct cttcgagctc gacgccgatc gcgcgccggc cgagggcttt ggaggcgacg
     1141 agggttgagc ccgagccggc gaacgggtca gcaatgatgc cgggcggaca agcctcgata
     1201 aggctctcca tgacgtcgag cggtttcgcg tgaggatggc ccgccctcgt ggcatagccg
     1261 ctgtatcccc cagcagccag ggcgcgcgtc gagaccactg cggacagcat tgacgtcgac
     1321 ttaggccagc ggccaagcac gtcggcggcc agccactcgg tgacctccag gcaatccccg
     1381 tggtacagcg tcacgaacgg gtcggagtag tacggcttca cggctcgatc ccttctggag
     1441 caggcactgt gcccatcggt cgccagccga ggtcgagaat cttgtggacg agcacccagg
     1501 ccaccgtccc gtcgtcgcgg atgttccgca ggtgcgccca aaggtcgagc acctgcgcgc
     1561 cggtcggggg ttgctcgggc cgtgcgtagt tcttcggtga gcttcacggc tcgatccctt
     1621 ctggtatgtc gtggcctgcg ttgcggtggg cggccaggtg tgcttgtagc cgtcgttcgg
     1681 tcaggtagta gctggtgccg ccgcgcagtc ggcattagcg cgacatcatg tgggatcacc
     1741 gccgtccagg gcgcggagga tggcccggcg ggccgccttg ttccacgagt tgccgtgcga
     1801 ccaggagtca cactgcgccc ggacgcgctg gttggcctgc tcggcagcct cagcgcgcgc
     1861 ggtgagagcc ttgaccgctg cgagggcatc cgggccggtg atgcgcccac ccagccggat
     1921 gccgagttcg gcgccaagct cttgcagccc gtcgatgact gggagggcct cggccttcca
     1981 gtggctcagt cgatccacct cggccaggag ttcggccagc caagtcggcg ctgacgccac
     2041 gaactccaag gtgtcccgcg acccgtagag gtcgccgtcc tcggtccagc gtccatgcac
     2101 cccggagtcc tcgtcgctgg tgccgattcg ggtcgcggcg tggatgtcct cggtgccgac
     2161 gagcggcccc cagccattcc agatgcacca ctgctcgcca gtcgggttga ccttagccag
     2221 tgcggccttg atgtcgtcgt ggctcatgac tggacctccg ggggaagcac ttccgccagc
     2281 cgattctcgg ccgcggcgta gccgtactcg ctcagcctcg acatcggcca gcttggcttt
     2341 caggtaggcg atctcgacgg tcgggtcatc gccctcaagg aatcggcgga caccgtccac
     2401 cagggcaacc agatgcccgt agcgtccgtc ctggcagtcg tcgtagaggc gctgtagctc
     2461 ttctcgggtc atggtcatgc ccccagtccg agcagtgtgg cggcggcttc gacctggtcc
     2521 cagaggctcc ggtcgccgcc gttgcggtca gggtgagccg cggcgcgggc tcggcgcagc
     2581 agtgccgcct cgggcagctt cggtgactcg ccggcgatcc gggcgagcac ctcgaccggt
     2641 gactcggccg accggccagc ctcgatctgt agccagccct tgtactgctc gccggcggtg
     2701 gtggtgatgc cgtaccggtc gacggcacgg agcgcttcga ggccgagggc gatggagcgg
     2761 acgttgtgct gccagtcctc gcacacgtcg gtctgccacg acagggtgcc gcggggcgtg
     2821 tcgacgatca gccggacgcc ggggaaggtc ggccgggcgt ccgcgcggag ttggccgttg
     2881 agacgcacgg acgcctcgcc gacgtcgacc tgcaggatca ccgcgtcggc gtccaggtgc
     2941 cccagttcac ggtccagcaa actcagggtc gccggccagg ccgccttgaa ggtccaccgg
     3001 gagcggcgct gttcggtgac cggctgcccc cagggcagct gcaacgggcg atgggtgaga
     3061 atcatgccgc ccgacccccc tgagtgagtt gggggagggc cgcgatctgc tgatcagcga
     3121 tcgcgacggc agccgcagac ggcttcaccg tcagggtcgg gctgcccggc accggctcgg
     3181 cgctcactcc gtccgggacg acaacctcac ccgtctccgg gtccacagct tcgccctgtc
     3241 gccggatcag ctcggtgaag ttgcctgaca cggccaccgt ggtgacgatc tcgtccgggc
     3301 gattggcctc cacccaggcc aggaaggccg caggatcggt gatgcggtag gtgggcttgg
     3361 gcttagtgac gtacaccatg cccaggtcgg tttcgtcgtc ccaggcggtc agccggtcac
     3421 ccggacgcat tgtgtacagc aaggcgtcgc gggcttcccg gtctatgccg tggaggtggt
     3481 cgagcaccgc cttgcggagg acggcgcggc tgatcaggtc ggtcatcggg tcacttcctc
     3541 aagtacacct tcgcagtgcg ggcagcggat ggcttcgggg tcacagtcgg cgtgcatcac
     3601 accgccgcga gcattcacac gtgtctcgtc gtcctcttcg atcgagccga gacagatcac
     3661 acagacgggc ttcatccctg ccccctcagc gaggcgatga gtttgcggcc ttccacctcg
     3721 gtcagggtgg agcgggactc gacgacgcgg cccagcacct tgcccatcgc ggcccgctga
     3781 tcctccggct tggagacgtg ccgctcaccc atcagcgcgt acaactgggc gcgggtcttg
     3841 tcggtgatcg gctcatccgc cggcggggtt gtttcgggcc atgcctcgga ttccctaggc
     3901 gcttctcggc gctgctgggc gcgcggcttg gatgccaggg ctccgtcgtc gtcggcgtcg
     3961 gtcacgatcc ccgtcaggca tccgagcatg tagcgccgtg cgtacgtcag ggatgagccg
     4021 atctcctgag gcgtggagcc cgtcagagga aggacgccct cgatcctctc gccggagatg
     4081 tgcaacaaga cgccgcgcag cgtcatcctc ggcgggtcaa cctcggggag gcaagtgaaa
     4141 ctcagcccat gcttggacag caacgggtag gccgccgcag cgatgtccgc caggtcggca
     4201 tagctgtagc tgtagctgcc gccgctcttg gtggggacct tcgcggtctt gtccttcgcc
     4261 accttcgggg cctcgccctg gaaggcagcc agcgccttgg cgagctcgcc gctcatcgcc
     4321 gcctcctact ccgcacacac cgagggcacg gctcagcacc agcacccaaa caggtgccgc
     4381 acagatgacc gccatggcct attccggtgc cgcgacaatc ccggcaatca acctcacccg
     4441 tatcgccaca cgcatcgcag agtgtttcgt cggtcacggc ggtcatgatg acctctcttc
     4501 atgtgctcgt agtccgtggt ggcagaagtc agcgatggcg gcgatgatgg tcagcagtgc
     4561 gcaggcgaag atgaggccgg tcacgagttg gcccaccgtt cggctatccg gactgcctca
     4621 gcgagacgtc gctgctcgtc ccacagggtc gccatgcggc tgatgttcga gtcggacatg
     4681 acccgcttct gccatctggc ggtggtggtg cagccgacgc caccaaaggc ggcggggtca
     4741 tcggtgggcg gcttcggatc gcggcgttcg atctccgctt cgaccttggc cagttgggcg
     4801 cgcagcttcg gcagcttgct gatcgagtcg gtcttgcgct gcacgttccg ggcggcctcg
     4861 cgggcgatgc gctggtccag cctccggcgc gctacgacct catgccgggg gcacaggtgc
     4921 ggctcggctc cttcaggtcg cccacacgcc ttctcagcct tctccatcca cgcccagcag
     4981 gtgcctgccg ggtagtgatc ggagatcgct agagtcaggt agacgatgct cattgcagcc
     5041 actccgcagc cttgacaccc agcatgatca gaccgaccca tgcgacggca ccgacgatca
     5101 gggcccccag ccagccacgg gcagcccggc tggccttccg acgatgctca cgatccgcgt
     5161 gagcccgcac ctcggcagac acggcagtca cttcgcagcc tccgcccaag ccgcaagctg
     5221 ccgctcacac aggtcagcga acgccaggat gcgcggccga aacttggcac gccggtccgc
     5281 atcggcatgc gacggccagt gctcgccctc gatgagcttg cggagcttgg cgacatcgcc
     5341 ctcgaagcag ccgatgacga tctggccgtc cttgccaagc cggccctcgt gatcctgcga
     5401 cgacacatgg cccaccacca tctggtcacg atccggtcgc accagcgagt cggcgccgat
     5461 gtaggcatgc caggtcttga cctcggtcag atcgaccgcg gtcatgtcga tgatgtgacc
     5521 ttcgccggtc agggtgacgc gctgtgacca gaggtggacg gcggtgaagc ggccggcctc
     5581 gacgctggac gactcccggg cctcgacgtg ggacgactcc cgggccacga cgcgggacga
     5641 ctcccaggcc tcgacgtggg acgaccccca ggcctcgacg cgggacgact cccgggcctc
     5701 gacgcgggac gacccccggg cctcgacgtg ggacgactcc cgggcctcga cgtgggacga
     5761 ctcccgggcc tcgacgcggg acgactccca ggcctcgacg tgggacgact cccgggcctc
     5821 gacgtgggac gactcccagg cctcgacgtg ggacgacccc cgggcctcga cgtgggacga
     5881 cccccgggcc acgacgcggg acgactcccg ggccacgacg cgggacgacc cccgggcctc
     5941 gacgcgggac gactcccggg cctcgacgcg ggacgactcc caggcctcga cgcgggacga
     6001 cccccgggcc acgacgcggg acgactcccg ggccacgacg cgggacga
//
LOCUS       DAOMZY010000223         6015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_12389_length_6015_cov_6.055369, whole genome shotgun sequence.
ACCESSION   DAOMZY010000223 DAOMZY010000000
VERSION     DAOMZY010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12389_length_6015_cov_6.055369"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..2793)
                     /locus_tag="PLK64_12040"
     CDS             complement(169..2793)
                     /locus_tag="PLK64_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HPV80523.1"
                     /translation="MPFPLRAVRGAWLGVSHVAGSAVRKVGHSAAELEPEHRRDGVGF
                     LLIAVAIVVAAREWWGFQGVIGDVVHAVAAGTFGRIAYALPLVLLVFGLRLLRAPDDD
                     ATTNRVVVGTLTLTFAACGIAHLADGIPTPPAGADGMRAAGGIFGFLASSPLAAAVSP
                     VGALVLLLMLGLFGLLVITATPVRMIPQRLREVRDRILGRAPAPTETDDGAATGATKR
                     SRRPVIDLAAKRDGDDAFEQAAEAPGEGAGRKGLRPGQRRPEPEPAIESTDPTAHDAP
                     PVEPATRAGGGAGAGKRKAELIAPPTSTLPLRGEQLSLAGDIAYVLPDNALLAPGAPH
                     KARSAANDRVVESLTQVFEQFEIDASVTGFSRGPTVTRYEVELGLATKVERVTALSKN
                     IAYAVASADVRILSPIPGKSAIGIEIPNTDRETVSLGDVLRSQTARANDHPMVMGVGK
                     DVEGGYVIANLAKMPHLLVAGATGAGKSSFVNSMITSILMRATPEEVRMILVDPKRVE
                     LTAYEGIPHLITPIITNPKKAAEALQWVVREMDQRYDDLASFGFKHIDDFNKAVRTGK
                     VTPLPGSERVITPYPYLLIIVDELADLMMVAPRDVEESIVRITQLARAAGIHLVLATQ
                     RPSVDVVTGLIKANVPSRMAFATSSLADSRVVLDQPGAEKLVGQGDALFLPMGTSKPM
                     RVQGAWVTESEIHGVVKAVTLQLQPQYREDVVVPVAASRQVEDDIGDDLEVLLQAAEL
                     VVTTQFGSTSMLQRKLRVGFAKAGRLMDLLESRGIVGPSEGSKAREVLVKPDDLPTTL
                     ALLRGEDVPAEAWVPEGVPGVLDGDSGDTAYVLDGDAHTKAQAGGRYPSDPLAGSDDV
                     PGWDEDEGGDAWELTGRG"
     regulatory      3061..3178
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00634"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class IV; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00634"
     gene            3215..4609
                     /locus_tag="PLK64_12045"
     CDS             3215..4609
                     /locus_tag="PLK64_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPV80524.1"
                     /translation="MYAASLATAEPLTATPLTRRPQGDFSAACTQRLPGTTADGLVVP
                     TSRGPRDYANLDHAASTPALTVVAEAVERTLRTYASVHRGNGFASRVTSEWYEQAREE
                     VARFIGAREDDLVVFTRTTTDSWNLLARTLPADTTVVVFASEHHSTLLPWGTRTSGAT
                     VTLPVPRTVEDGLAWLDSALAESTGAHTLVVVSGASNVTGEVWPVEQIVAVAHRHGAR
                     VALDAAQLAPHRAVDLAASGVDYVAFSGHKLYAPYGAGVLAGRADWLDAAEPYLVGGG
                     ATARVGQCGITWATGAARHEGGSPNVVGAVALAAACAVLQERRDDLARYEHTLAERLR
                     RGLEQIPGVHTHSLFGAGSDRVGVVAFTIDGLDSSLVSTALSVEHGIGVRDGKFCAHP
                     LVDALLADGDEGTPTTAVRASLGLASRPEHVERLVSAVTELAASGPSLTYTRGPQGWA
                     PESDPRVVDLPRPW"
     gene            complement(4725..5624)
                     /locus_tag="PLK64_12050"
     CDS             complement(4725..5624)
                     /locus_tag="PLK64_12050"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPV80525.1"
                     /translation="MRWLDQATFLVAVVQAVVPVAGIVIVATTLLAAVSRRRRTALVG
                     AIASVVVLVGAAPFVIPSGRAEPGPDDVVVMSSNLQYGRADAVALTERVRALDVDILV
                     LLEVTPSAVDLLRAAGLEAMLPFVSGHPRAGADGTMIRSRFPLAELPTPTFPGEVNLS
                     QPVARVDLPGTSSAPAGRPMLIRAVHPMPPTDPNLGFWRSALDGLATWAHSQPDGIPL
                     VLAGDFNASADHPGYRQVADGLVDAHRAVGAGWVRTWPLGRRWLPPFVQIDHILARGL
                     TVTGAGSEVIAGTDHALVWASWR"
     gene            complement(5695..5994)
                     /locus_tag="PLK64_12055"
                     /pseudo
     CDS             complement(5695..5994)
                     /locus_tag="PLK64_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNase J family beta-CASP ribonuclease"
BASE COUNT          870 a         2195 c         2093 g          857 t
ORIGIN      
        1 gggtgatgcg ggccggcgaa ggcgtggccc gacaggccca gcgagagcgc ggcccgtcgc
       61 aggcgacctt cgacgacctc gatcgttcgc cctgagtttg aagtgcgcga cggagtgcgt
      121 tcaagggggt gatcacccca cccatcgcac tccgtgccgc gctcagagtc agccgcggcc
      181 ggtgagctcc caggcgtcgc cgccctcgtc ctcatcccag ccgggcacgt cgtcggagcc
      241 ggccagcggg tcgctcgggt agcgtccgcc cgcctgcgcc ttggtgtggg cgtcgccgtc
      301 cagcacgtat gccgtgtcgc cgctgtcgcc gtccaacacg cccggcacgc cttcgggcac
      361 ccaggcctcg gccgggacat cctcaccgcg caggagcgcg agggtcgtgg gcaggtcatc
      421 gggcttgacg aggacctcgc gcgccttgga gccctccgac ggcccgacga tgccgcggga
      481 ctccagcaga tccatgagcc gaccggcctt ggcgaagccg acccgcaact tgcgctgcag
      541 catcgacgtc gacccgaact gcgtcgtcac gacgagctcg gccgcctgca gcagcacctc
      601 gaggtcatcc ccgatgtcgt cctcgacctg acggctggcg gccaccggaa caacgacgtc
      661 ctcgcggtac tgcggctgca gttgcagcgt gacggccttg accacaccgt ggatctccga
      721 ctcggtcacc caggcaccct ggacacgcat cggtttggag gtccccatcg gcaggaacag
      781 tgcgtcgccc tgacccacca gcttctcggc gccgggctgg tcgagcacga cccggctatc
      841 ggccagggac gaggtggcga acgccatccg ggagggcacg ttggccttga tgaggcccgt
      901 gacgacatcg acgctggggc gctgggtagc caggaccagg tggatgccgg ctgcccgcgc
      961 caattgggtg atccggacga tcgactcttc gacgtcccga ggcgcgacca tcatgaggtc
     1021 ggccaactcg tcgacgatga tcagcaggta ggggtagggc gtgatgaccc gctcactgcc
     1081 gggcagcggg gtcaccttgc cggtgcgcac cgccttgttg aagtcgtcga tgtgcttgaa
     1141 cccgaagctg gccaggtcgt catagcgctg gtccatctcg cgcacgaccc attgcagcgc
     1201 ctcggccgcc ttcttggggt tggtgatgat cggcgtgatg aggtgcggaa tcccctcata
     1261 ggcggtcaac tccacgcgct tggggtccac gaggatcatc cggacctcct caggcgtggc
     1321 ccgcatgagg atcgaggtga tcatcgagtt gacgaagctg ctcttgcccg ccccggtcgc
     1381 cccggcgacg agcaggtgcg gcatcttggc gaggttggcg atgacatacc cgccctcgac
     1441 gtccttgccg acccccatga ccatcgggtg gtcgttggcc cgggcggtct ggctgcgcag
     1501 cacatcgccc agcgacacgg tctcgcggtc ggtgttgggg atctcgatgc cgatcgcgga
     1561 cttgccgggg atcggcgaga ggatccgcac gtcggccgac gcgacggcat acgcgatgtt
     1621 cttggacaac gcggtgacgc gctcgacctt ggtggccaga cccagctcga cctcgtagcg
     1681 cgtgaccgtc ggcccacggc tgaacccggt gacggaggcg tcgatctcga actgctcgaa
     1741 cacctgggtg agcgactcca cgacccgatc gttggccgcc gaacgcgcct tgtggggtgc
     1801 gccaggggcc aacagcgcgt tgtcgggcaa cacgtaggcg atatccccgg cgagcgacaa
     1861 ctgctccccg cgcagcggca gggtactggt cggcggcgcg atgagttccg ccttgcgctt
     1921 gcccgcgccc gctcccccac ccgcgcgggt ggccggctcg accggcggtg cgtcgtgggc
     1981 ggtgggatcg gtcgactcga tggccggctc gggctcggga cgtcgttgcc cgggccgcag
     2041 ccccttgcgc ccggcgccct cgcccggcgc ctcggcggcc tgctcgaagg cgtcatcgcc
     2101 atcgcgcttg gcagccaggt caatgacggg ccggcgcgag cgcttggtcg cccccgtcgc
     2161 ggcaccgtcg tcggtttcgg tcggcgccgg ggcccgcccg aggatccggt cacgcacctc
     2221 acgcaaccgc tgcgggatca tccgcaccgg ggtcgcggtg atgacaagca gcccgaaaag
     2281 gccgagcatg agcagcagaa cgagcgcccc gacgggcgag acggcagcgg ccagcgggct
     2341 ggaggcgagg aagccgaaga tgcctccggc cgcacgcatt ccgtcggcac cggcaggcgg
     2401 cgtcggtatg ccgtccgcca ggtgggcgat cccgcaggcg gcgaaggtca gggtgagggt
     2461 tccgaccacg acccggttgg tcgttgcgtc gtcatcggga gcgcgaagca ggcgcaggcc
     2521 gaacacgagc agcaccagcg gcagggcgta ggcgatccgt ccgaacgtcc cggcggcgac
     2581 agcatggacg acgtcgccga tgaccccctg gaagccccac cactcgcgcg ccgcaacgac
     2641 gatcgcgacc gcgatgagca ggaagccgac accgtcacga cggtgttccg gctcgagctc
     2701 ggcggcggag tggccgacct tgcggacggc gctgcccgcg acgtgcgaca ccccgagcca
     2761 ggctccgcgc acggcccgca gcgggaacgg cataccgccg cccttgggcc gccgcgagga
     2821 ccggggggcg cgccggggtg tgccggtgcg cgaccggggg tttgcggagc gcgcggacgt
     2881 gctccgggtg gtggtctgtg ggcgtgcgga cgctccagac ggtcgtgtcg ccatgatccg
     2941 gaggttacgc caccagccac gccaacccca tgcgccacac gcgtcacagc cgcgaaattc
     3001 cgtctcagaa cccggaccag gcttcacagg ccgcatcacg tgccgttatc ttctacccaa
     3061 ggtcatgagt gtcagcgcac agccccggct tgctgatcgg caaccctcct ctgcggcggg
     3121 gtgctccggg tgacgaccgg gcttgtcagc ggacctcgct gaccggcaag cgcggacccg
     3181 tcacccgggc ccacggaccc gcaggagacc tccgatgtat gccgcatcgc tcgccaccgc
     3241 cgagccgctg accgccacgc cgctcacccg ccgtccgcag ggcgacttca gcgccgcgtg
     3301 tacgcagcgg ctgcctggga cgacggccga cggcctggtc gtccccacct cccgaggccc
     3361 gcgcgactac gccaacctcg accacgccgc gtcgaccccg gccctcaccg tggttgcgga
     3421 ggccgtcgag cgcaccctgc gcacctacgc ctcggtccac cgcggcaacg gcttcgccag
     3481 ccgcgtgacc agcgagtggt acgagcaggc ccgcgaggag gtcgcccgct tcatcggcgc
     3541 ccgcgaggac gacctggtgg tcttcacccg cacgacgacc gattcgtgga acctgctggc
     3601 ccgcaccctt ccggccgaca cgaccgtagt cgtcttcgcc tccgagcacc actcgaccct
     3661 gctcccctgg ggcacgcgca cctcgggcgc gaccgtgacc ctcccggtgc cgcgcaccgt
     3721 cgaggacggg ttggcctggc tcgacagcgc cctcgccgag tcgaccgggg cgcacaccct
     3781 cgtcgtcgtc tccggcgcct cgaacgtcac cggtgaggtc tggccggtcg agcagatcgt
     3841 cgccgtggcc caccggcacg gcgcccgggt cgccctcgat gccgcgcagc ttgccccgca
     3901 ccgcgcggtc gacctcgccg cctccggcgt cgactacgtc gcgttctccg gccacaagct
     3961 ctacgccccc tacggcgcag gcgtcctcgc cggtcgggcc gactggctcg acgccgccga
     4021 gccctacctc gtcggcggtg gcgcgacggc tcgcgtcgga cagtgcggga tcacctgggc
     4081 caccggtgcg gcgcgtcacg agggcggcag ccccaacgtc gtcggcgccg tcgccctcgc
     4141 cgcggcctgc gccgtcctgc aggagcgccg ggacgacctc gcgcgttacg agcacaccct
     4201 cgccgagcgg cttcgccgcg gcctggagca gatccccggc gtgcacaccc actcgctctt
     4261 cggcgccggc tccgaccggg tcggcgtggt cgccttcaca atcgacggtc tggactcctc
     4321 cctcgtgtcg accgcgctgt cggtcgagca cgggatcggc gtccgcgacg gcaagttctg
     4381 cgcccacccg ctcgtcgacg cgctcctcgc ggacggcgac gagggcaccc ccacgacggc
     4441 cgtccgggcc agcctgggcc tggcctcccg ccccgagcac gtcgagcggc tcgtctcagc
     4501 ggtcaccgag ctcgcggcct ctggcccaag cctgacctac acccgtgggc cgcagggctg
     4561 ggcgcccgag tccgacccgc gcgtcgtcga cctcccccgc ccctggtagt cgggtcggtt
     4621 agtccggcag gttagttcgg tcggttcgtc cggcaggtta gttcggtcgg ttagttcggc
     4681 cggttcgtcc ggccggttag ttcggccgtt agtccggccg gcgctcagcg ccaactcgcc
     4741 cagacgaggg cgtgatcagt gccggcgatg acctccgagc cggcgccggt gaccgttagt
     4801 ccccgagcca ggatgtggtc gatctggacg aacggcggca gccatcgccg ccccagcggc
     4861 caggtgcgga cccacccggc gccgacggcc cggtgggcat cgacgaggcc gtcagcaact
     4921 tggcgatagc ccggatggtc ggcagaggcg ttgaagtccc ccgccaacac caggggtatg
     4981 ccgtccggct ggctatgcgc ccacgtcgcc agtccgtcga gtgctgagcg ccagaacccg
     5041 aggttcggat cggtcggggg catcgggtgg acggccctga tcaacatcgg gcgcccggca
     5101 ggggccgagc tcgtgccggg gagatccacc cgagcgaccg gctggctgag gttgacctca
     5161 ccgggaaagg tcggcgtcgg cagttctgcg agggggaacc ggctgcggat catcgtcccg
     5221 tcagcgcccg cacgaggatg ccccgagaca aagggcagca tggcctccaa ccctgcggcg
     5281 cgcagcaggt cgacggcgga cggagtcacc tccagcagga cgaggatgtc gacgtccaac
     5341 gcccgaaccc gctcggtgag ggcaaccgcg tcggcccgtc cgtattgcag attgctcgac
     5401 atgacgacga cgtcgtccgg ccccggctca gcccggcccg aggggatgac gaacggcgcg
     5461 gcaccgacga ggacgaccac cgacgcgatg gccccgacca gggcggtccg ccgccgccgc
     5521 gacacggcgg ccagcagcgt ggtcgccacg atgacgatcc ccgcgacagg gacgacggcc
     5581 tggacgacgg cgacaaggaa ggtcgcctgg tcaagccagc gcacggcata catcgtggcg
     5641 gcgccggcca gcgccgcggc gagcagcccg ctgccgagcc ggtggcgcgc caggtcaggc
     5701 ctcgatgacg atcgggatga tcatcggacg gcgccggatc ttgccgcccg cccagccgcc
     5761 gacggtgcgc cggatcacct gctggagttg atgttcgtcg gtgatgcccc cctcggtggc
     5821 cgcgatgatc gcggcctcga ccttgggaat gacctgatcg aagatctgct cgtcctcggc
     5881 gaagccgcgg gcggtgatct cggggccggc tgcgatcttg ccggtcgccg cgtcgatgac
     5941 gacgatgcag gtgatgaagc cttcgtcgcg caggatccgc cggtccttga gcagaaggct
     6001 tcatcacctg catcg
//
LOCUS       DAOMZY010000224         5999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_12437_length_5999_cov_6.965511, whole genome shotgun sequence.
ACCESSION   DAOMZY010000224 DAOMZY010000000
VERSION     DAOMZY010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12437_length_5999_cov_6.965511"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="PLK64_12060"
     CDS             <1..907
                     /locus_tag="PLK64_12060"
                     /inference="COORDINATES: protein motif:HMM:NF040070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6350 family protein"
                     /protein_id="HPV80526.1"
                     /translation="PMLVWGLAAAFTAYQLRRLVAHTDERPLRLLPGFLGGYAAAAAL
                     VGLLTLAGPVRPTPAGLLGALSVPLVAAAVVALREDDDLFDRLPQWAARALRPAGWGV
                     VTLAALAAALLLGMLVVRWPTLSSLDAAVGAHGAGEVGLIVGQLLFLPDLLVWALSFI
                     AGPGFQVAAGGTVSVSGAAPGLLPMIPALGVVPSDGLYPAAVTLVLLLPLAAGALVAW
                     HAGRQWSRLARWQDKASTVTCAVVLVDLVVLGAALLASGPAGSARLVHVGPQPWVLAG
                     AMLVELVIGAGLTLAVDVARRRWVG"
     gene            1062..1919
                     /locus_tag="PLK64_12065"
     CDS             1062..1919
                     /locus_tag="PLK64_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006215300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV80527.1"
                     /translation="MTDTANFLAPGSGGGLLDVFHRRYLLRLLVHKELKARYHGSVLG
                     MLWSYIKPAVQFGVFYFVIGVFFKMNYAVENYAVYLFSGIVAMNFFSEAFGNATRSIT
                     GNWALVKKIYLPRELFPVASVFVSFIHFIPQVLVLLVGCFVTGWHPSGWQLLAGVGAF
                     FVLAVFALGVGLIFGALNVVFRDFENVVDLILMVATWASPVIYTWTAARDALGDGALW
                     WLYQCNPLTTVVEIFHWTFWAPTTGGAAPMPAQTWTFVIIATGISVAVLAIGQLVFRR
                     FDGRFAQEL"
     gene            1920..2696
                     /locus_tag="PLK64_12070"
     CDS             1920..2696
                     /locus_tag="PLK64_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012903229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV80528.1"
                     /translation="MSDGGEVSMSSGRELDRIRINEVSKHFTLRHTRAVKEVVVKGLK
                     GRRRGLSEQFTALRDISLTVGEGEAVALLGRNGSGKSTLLKLISGVMGADEGRIEVRG
                     NIAGLIDVGAGFHPDLTGRENVFLNGAILGMTEAKIHEQFDRIVAFSEIEDFIDNEVK
                     FYSSGMFLRLAFAVAVHTDPDIFLVDEILAVGDEPFQHKCLDRIRELRDEGKCLVIVS
                     HDLDMVSRFTERGIVLDKGEVIHDGPIDDAVTLLRRRAEG"
     gene            2774..3955
                     /locus_tag="PLK64_12075"
     CDS             2774..3955
                     /locus_tag="PLK64_12075"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPV80529.1"
                     /translation="MTDFIPFALPDIGDAEIEAVVSTLRSGWLTTGPNAAALEREFVE
                     AMHPSAHVGAAQVQAVAVNSATAGLHLALEALGVGPGDEVLVPTWTFTATAEVVRYLG
                     ATPVLVDVHPDTLNIDLARAAEAVTPRTKAIMPVHFAGLPVPRGALADFAAAHGLRVI
                     EDAAHSHPVDSEGMPVGLGTSDAVVFSFYATKTMTSGEGGMVVSADPQLIARMRTMRL
                     HGISRDVFNRYTSTAPSWHYEVVAPGFKYNLTDVAASMARVQLTRSVAMRERRSAIAD
                     HYDSALAGLPLRLPSRPAPGTQHAWHLYALRLTDDAPMGRDDFIQRMSDAGVGCSVHF
                     IPLHHHPYWRDLRGADAPALPVADAQFARAVSIPIFSSMTDAQVERVGQTVRAVLEGR
                     S"
     gene            3952..4605
                     /locus_tag="PLK64_12080"
     CDS             3952..4605
                     /locus_tag="PLK64_12080"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPV80530.1"
                     /translation="MNSRITGADGAPFGRRGAGVDAKRAFDVAVAAGMLAVLSPALAV
                     IAVLIKRDDPAGPVLFTQERVGLSGIPFRIHKFRTMRPGAPGLQVTSDGDPRVTRVGA
                     VLRKTKLDELPQLWDVLVGRMSLVGPRPEVPKYVALWPAQHREVILSVRPGITDPASV
                     RWRNESEELAQAPDPEAHYVEVLLPQKAAMYAEYVRTRSFWGDVLVLFRTAVAVGRH"
     gene            4813..5409
                     /locus_tag="PLK64_12085"
     CDS             4813..5409
                     /locus_tag="PLK64_12085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPV80531.1"
                     /translation="MTVRIAPSADVSPQASIGDGSAIWHLAQVREGAVLGGNCIVGRG
                     AYVGTGVVMGDNCKLQNYALVYEPARLGHGVFVGPAVCFTNDHFPRSVTPDGQLKRGD
                     DWEAVGVTCLDGASIGARSVCVAPVTIGRWALVAAGSVVVKDVPDFALVAGVPARRIG
                     WVGRAGVPLTDTGDGRWICPETGAAYMEHDGTLTEATP"
     gene            5406..>5999
                     /locus_tag="PLK64_12090"
     CDS             5406..>5999
                     /locus_tag="PLK64_12090"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPV80532.1"
                     /translation="MTDFIPPAKPIVGDDEIAAVTRVIRSGMLAQGPEVKAFEEEFSA
                     HFGLGRACVAVNSGTSGLHLGLLSCGVGAGDEVIVPSFTFAATANSVALTGATPVFAD
                     IEPDTFCLDPASVEAAITERTVGIMPVHLYGHPASMGPLQEIATRHGLRLFEDAAQAH
                     GASLDGTPVGAFGQFAMFSLYPTKNMTSGEGGMVSVAD"
BASE COUNT          841 a         1964 c         2068 g         1126 t
ORIGIN      
        1 tccgatgctc gtgtggggac tggccgcagc gttcacggca taccaactgc ggcgtctggt
       61 cgcgcacacc gacgaacgtc ccctgcgcct ccttccgggc ttcctcggcg ggtatgccgc
      121 cgccgccgcg ctcgttggcc tgctcaccct ggccggcccg gtgcggccga cacctgccgg
      181 gctgctcggt gccctgtcgg tccctctggt cgcggccgcc gtcgtggccc tgcgggagga
      241 cgacgatctg ttcgaccggc tcccgcagtg ggccgcgcgc gccctgcggc cggccgggtg
      301 gggtgtggtg acgctcgccg ctctggccgc cgcgctgttg ctcgggatgc tcgtcgtgcg
      361 ctggccgacg ttgagctcgc tcgatgcggc cgtgggagcg cacggcgcag gcgaggtggg
      421 gctgattgtc ggccaactcc tcttcctgcc cgacctgctc gtctgggcgc tgagcttcat
      481 cgccggcccg ggtttccagg tggctgccgg cggcaccgtc agcgtctcgg gtgcggctcc
      541 cggtctgctg ccgatgatcc cggcgctcgg tgtcgtgccg tcggacggtc tctatcccgc
      601 agcggttacc ctcgtgctgc tccttccgtt ggccgcgggc gcgctggtcg cctggcacgc
      661 aggacggcag tggtcccgcc tggcccggtg gcaggacaag gccagcaccg tcacgtgcgc
      721 cgtcgtgctg gtcgacctgg tcgtcctcgg ggcggcgttg ctggccagcg gtccggccgg
      781 ctcggcgcgg ctggtgcacg tcggtccgca gccctgggtg ttggccgggg ccatgctcgt
      841 cgagctcgtg atcggggcgg gcctcacgct cgcggtcgac gtcgcccgcc gtcgctgggt
      901 gggctgacgt ggttcatccg cgctggtcgt gccctcaggt agtctgtgcg tgcttcgcgg
      961 tcggccagga cggcgtcgta ccctgcccaa cgggcaggtc gcggcggccg gatggcggag
     1021 cgaattgcac gcagaccgca tccacgtgag ggccgaatca catgacggac acggcgaact
     1081 tcctggctcc gggttccgga ggtgggctgc tcgacgtctt ccaccgccgc tacctgctgc
     1141 gacttctggt tcacaaggag ttgaaggctc ggtatcacgg gtcggtgctg gggatgttgt
     1201 ggtcgtacat caaacctgcg gtccagttcg gagtgttcta ctttgtcatc ggcgtgttct
     1261 tcaagatgaa ctatgcggtt gagaactatg cggtttacct gttctcagga atcgttgcga
     1321 tgaacttctt tagcgaagcg ttcggcaatg cgacccgctc gatcaccggc aactgggcgc
     1381 tcgtcaagaa gatctacctc ccccgcgagc tcttcccggt cgcatcggtt ttcgtgtcgt
     1441 tcattcactt catcccccag gttctcgtcc tccttgtggg gtgtttcgtc acgggatggc
     1501 acccgtcggg gtggcagctc cttgccgggg tcggcgcctt ctttgtcctc gcggtcttcg
     1561 cgctgggcgt cgggctgatt ttcggcgcgc tcaacgttgt cttccgagac ttcgagaacg
     1621 tcgtcgacct catcctcatg gtggccacct gggcctcgcc ggtcatctac acgtggaccg
     1681 cggcgcggga cgccctcggc gatggtgcct tgtggtggct ctaccaatgc aaccccctca
     1741 ccaccgtcgt ggagatcttc cactggactt tctgggcccc gacgacaggc ggcgcggccc
     1801 cgatgcccgc gcagacctgg accttcgtca tcatcgccac aggaatctcc gtggcggtcc
     1861 tggcgatcgg tcagctcgtc ttccgtcgtt tcgacggccg atttgcgcag gaactctaga
     1921 tgagcgacgg cggagaagtg tcgatgagta gcggacgtga gttggaccga attcgcatca
     1981 atgaggtgag taagcacttc accctgcggc atacccgcgc cgtgaaggag gtcgtggtga
     2041 agggcctcaa ggggaggcgc cgcggcttga gcgagcaatt caccgcgttg cgggacatct
     2101 cgctgaccgt cggcgagggc gaggcggttg cgctgcttgg ccgaaacggc tcggggaagt
     2161 cgacgctact caagttgatc tcgggcgtca tgggggcaga cgagggccgg attgaggtgc
     2221 gcggcaacat cgccggtctg attgacgtgg gagccgggtt tcacccggat ctcacggggc
     2281 gagaaaacgt cttcctcaat ggggccattc tcggcatgac cgaggcgaag atccacgagc
     2341 agttcgatcg gatcgtcgcc ttcagcgaga tcgaagattt cattgacaat gaggtgaagt
     2401 tctactcctc tggaatgttc ttgcgtctgg ccttcgcggt tgccgtgcac actgatccgg
     2461 atatcttcct cgtcgatgag atcttggctg tcggtgatga gccattccaa cacaagtgtc
     2521 tcgaccggat ccgcgagctg cgcgacgagg gtaagtgcct cgttatcgtc agtcacgact
     2581 tagacatggt ctcccggttc accgaacgcg gcatcgtgct cgacaagggc gaagtgatcc
     2641 atgatggtcc gatcgatgac gctgtgaccc tgctgcgtcg gcgtgcggaa ggctgaccgg
     2701 tgggccgcga cgacggccgg gagacgggcg ccatacattg ggttcgcacc catccccacg
     2761 gaaggcagga atcgtgaccg acttcattcc cttcgcactg ccggatatcg gtgacgcgga
     2821 gatcgaggcc gtcgtgtcga cgttgcgctc gggctggttg acaacagggc ccaacgcggc
     2881 ggccttggag cgggagttcg tcgaggcgat gcacccgagc gcccacgtgg gtgcggcgca
     2941 ggtgcaggca gttgcggtga actcagcgac cgcaggcctg cacctggcgc tggaggccct
     3001 tggggtcggc cccggcgacg aggtcctcgt cccgacctgg accttcactg cgaccgcgga
     3061 ggtcgtccgg tacctcgggg cgacccctgt cctggtcgat gtccatcccg acaccctcaa
     3121 catcgacctt gctcgcgccg cggaagccgt gaccccccgg accaaggcga tcatgcccgt
     3181 ccacttcgcg gggttgccgg tgccgcgcgg ggcgctcgca gacttcgccg cggcgcatgg
     3241 actgcgggtc atcgaggatg cggcgcactc ccacccggtc gactcggaag gtatgccggt
     3301 cggtctcggc acgagcgacg ccgtggtctt cagcttctac gcgaccaaga cgatgacctc
     3361 tggcgagggc ggaatggtcg tcagcgccga cccgcaactg attgcccgga tgcgcaccat
     3421 gcggctgcac gggatcagcc gggacgtctt caaccgctat acctcgacgg cgccgtcctg
     3481 gcactacgag gtcgtcgctc cggggttcaa gtacaacctc accgatgtcg ccgcatcaat
     3541 ggctcgggtc caactgaccc gctcggtggc gatgcgggag cgccggtcgg ctatcgcaga
     3601 tcactacgac agcgcgttgg cgggcctgcc cctgcggctt ccgtcgcgtc cggcccccgg
     3661 aacccagcat gcctggcact tgtatgccct ccggctcacc gatgatgcgc ccatggggcg
     3721 ggacgacttc atccagcgga tgtcggacgc gggcgtcggc tgctcggtgc acttcatccc
     3781 gctccaccac cacccctact ggcgagacct ccgtggcgct gacgccccgg ctctgccggt
     3841 ggctgacgcg cagtttgctc gggcggtgag cattccgatc ttctcgtcga tgaccgatgc
     3901 tcaggtcgag cgcgtcgggc agacggtgcg ggcggtgttg gaggggcggt catgaactcg
     3961 cggatcactg gtgccgacgg cgcccctttc ggccgacgcg gcgcgggggt ggacgccaag
     4021 agagcctttg acgtggctgt tgcagcgggg atgcttgcgg tgctatcgcc agccctcgcg
     4081 gtcatcgccg tactcatcaa acgagacgat cccgccggcc cggtgctgtt tacccaggaa
     4141 cgggtcgggt tgagcggcat accgtttcgg atccacaagt tccggaccat gcgccccggc
     4201 gcgcccggtt tgcaggtgac ctccgacgga gacccacggg tcacccgggt cggcgcagtc
     4261 ctgcgcaaga ccaagctcga cgagctgccc caactgtggg acgtgctcgt cggcaggatg
     4321 agcctggtcg gcccccgacc ggaggtgccg aagtacgtcg cgctctggcc ggctcagcac
     4381 cgtgaggtca ttctttcggt gcgccctggt atcaccgacc cggcgagcgt ccgctggcgc
     4441 aacgagtcgg aggagcttgc ccaggcaccc gatccggaag cccactacgt cgaggtcctc
     4501 ctgccgcaga aggctgccat gtatgccgag tatgtccgga cgaggtcgtt ctggggcgat
     4561 gtgctcgtcc tcttccggac cgcggttgcc gtgggtcgcc actaaccggc ccgttcagtg
     4621 ccgattagac tgatcgcgct ccgacggcgg tgcgtcggac gacgcgtccg acggcgggcg
     4681 acccaaggga gacaggtggc cctcgttgga gggatcacca tgggggtgga gccgcatttt
     4741 gccggcctag cccaattgtc gtggagccgc tccacctgtc gaccgacccg atgccggcca
     4801 cggaggtctg agatgacggt tcgaatcgcc cccagtgcgg acgtctcacc gcaggcttcg
     4861 atcggcgacg gctcggccat ctggcacctt gcccaggtcc gcgagggcgc ggtactcggc
     4921 ggcaactgca tcgtcggccg cggcgcctat gtcgggacgg gcgtcgtcat gggcgacaac
     4981 tgcaaactgc agaactacgc actggtctac gagccggcgc ggctcgggca cggggtgttt
     5041 gttggcccgg cggtgtgctt cacgaacgac cacttcccgc ggtcggtgac cccggatggc
     5101 cagctgaagc gaggcgacga ctgggaggct gtcggcgtga cctgcctgga tggcgcctcg
     5161 atcggcgccc ggtcggtctg cgtcgcgccg gtgacgatcg ggcgttgggc gctagtcgcc
     5221 gctggatcgg tcgtcgtcaa ggacgttcct gactttgctc tcgtcgccgg cgtgccggcc
     5281 cgccggatcg ggtgggtcgg gcgggccggc gtgccattaa cggacacggg cgacggacgc
     5341 tggatctgcc ccgagaccgg ggcggcatac atggagcacg acggcactct gacggaggca
     5401 accccatgac cgatttcatc cccccggcca agccgatcgt cggtgacgac gagatcgccg
     5461 cagtgacccg ggtgatccgc tccgggatgc tcgcccaggg ccccgaggtg aaggccttcg
     5521 aggaggagtt ctcggcgcac ttcgggttgg ggcgcgcgtg tgtcgccgtc aactcgggca
     5581 cgtcagggct gcacctgggg ttgctgtcct gtggcgtcgg ggccggtgac gaggtcattg
     5641 tgccctcgtt caccttcgcc gcgaccgcca actcggtggc cctgaccggc gccactccgg
     5701 tgtttgccga catcgagccg gacaccttct gcctcgaccc ggcctcggtc gaggcggcga
     5761 tcaccgaacg cacggtcggc atcatgcccg tgcacctcta cgggcacccg gccagcatgg
     5821 ggccgttgca ggagatcgcc acccggcacg ggctgcggtt gttcgaggac gccgcgcagg
     5881 cacatggtgc gtcgttggac gggaccccgg tgggggcctt cggccagttc gcgatgttct
     5941 cgctctaccc gaccaagaac atgacgtccg gtgagggcgg catggtctcg gttgccgac
//
LOCUS       DAOMZY010000225         5987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_12459_length_5987_cov_5.409811, whole genome shotgun sequence.
ACCESSION   DAOMZY010000225 DAOMZY010000000
VERSION     DAOMZY010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5987
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12459_length_5987_cov_5.409811"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PLK64_12095"
     CDS             complement(<1..484)
                     /locus_tag="PLK64_12095"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HPV80533.1"
                     /translation="MSNPSSHAAAHPVSPALTQGPVLAHVVRCGVVESIHRGSAVVTR
                     ADGSIEQAWGDPTGLVFPRSASKPIQAAAMVAAGLDVAPHQLALVAASHSGEAFHLAT
                     ALEILHGAGLTEAHLLNTPDYPVDEQARDEWIRAGHLKTSLAQNCSGKHSGMLATTAA
                     N"
     gene            complement(667..1440)
                     /locus_tag="PLK64_12100"
     CDS             complement(667..1440)
                     /locus_tag="PLK64_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80534.1"
                     /translation="MARKTRMDEMKKDLQKTATEARTQLMAVVGATDLAVEKIRVAQE
                     HFVQVAREMDAKSLQKDAERFQKDVKKVQKNLAKIQADLTKRLSKTAKDVQTVPANAV
                     GHSIELATKAQATVDELAERGEALVQRIRTTRSTQDLKSQVETTVAAGKGAVTTARKA
                     AVSTERAAKATIKSAAEEAKVVAAKVAETVEKDAETVASTVKASAARTRAAARTTTTT
                     AQAGVKRTTARTRATGTGVRKTAAKTTTAAKTAAKKVGD"
     gene            complement(1456..1881)
                     /locus_tag="PLK64_12105"
     CDS             complement(1456..1881)
                     /locus_tag="PLK64_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPV80535.1"
                     /translation="MTPTLKLDLGSYLREQRRAAKLSLRQLSELAGISNPYLSQIERG
                     LKKPSAEILQQIARGLEVSAESLYVRAGILDESSPTSAPSVLAAIAADGRLTDRQRQV
                     LVDVYRSFVDIADEPRTAPATGSAPDGDVATQPTETPES"
     gene            1958..3487
                     /locus_tag="PLK64_12110"
     CDS             1958..3487
                     /locus_tag="PLK64_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HPV80536.1"
                     /translation="MAGLLVAGTSSDAGKSLIVAGLCRAWARQGVRVAPFKAQNMSNN
                     SMVCADGSEIGRAQHLQAIAAGVEPSALHNPVLLKPSTDRRAFIVLRGQPGGTLEAGE
                     YVGGRTRLADAAYEAYAVLAATHDLVVCEGAGSPAEINLRAGDYVNFGLAQRFGLPVV
                     LVGDIDRGGVLASIFGHWGIVDDADRALLAGYVINKFRGDQSVLDPGLDELTRRTGLR
                     CFGVVPWLDQVWLDSEDALAFAKWPRLPGRAGTLRVAAIRLPRISNATDLDAIASEPG
                     VDVFATAEPADIEHADLVVLPGSRATVADLEWLRRRGLADALAQRAAAGRPILGICGG
                     YQMLTHEIDDPVESPSGGCAGLGLLPARVRFQPEKVLGRPRGRWRGHDVVAYEIHHGV
                     VEPLAGSGGEGGLGGEPLAGSRGERSERGAGSEPFLDGIRDGAVWGTIWHGAFENDGF
                     RRAWLAEIAAAVGSTWVPRTDAPGFSERRERMLDDLADALEACVDLPALLAAARGATG
                     P"
     gene            complement(3489..4343)
                     /locus_tag="PLK64_12115"
     CDS             complement(3489..4343)
                     /locus_tag="PLK64_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HPV80537.1"
                     /translation="MMSASSAVGTLITVAPTGAETAKADVPQLPTTLDELVETARRCE
                     AAGAGLIHIHVRDREHRPTLDAAYLRDAVVAVREATDLVVQLSTGGSVHDPLEKRLTV
                     LDADPDSCSLTCGTTNFGDDIFLNPWGFMVDLYRLAQEREVVPEFELFELGHVHALRR
                     LIDTCGLPFGGKVHVDFVTGVPGAMPGTVEALLAGVAMLPAEVTSWSATGIGRAHLPI
                     LGAALAAGGHLRVGMEDNLFVARGQVAQHNVEFVERAATLAGLMQRPAFSTTQARELL
                     GIKDRRAR"
     gene            complement(4421..5512)
                     /locus_tag="PLK64_12120"
     CDS             complement(4421..5512)
                     /locus_tag="PLK64_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HPV80538.1"
                     /translation="MTGHAVPSPLLDAPGAVAAEGVDAGVAGHYGDPMREQRLLTEGL
                     AAVDLSNRGVVTVTGPDRLSWLNSMTTQALTGIEPGVGTESLVLDPHGHVEHALHLVD
                     DGTTTWITVEPGTAPALVAWLERMRFMLRVEVADVTDRYAVIGEPFGAVAAPGEPVVW
                     VDPWPGVIDLTPSGRAGRIAAYGPVDGHPAQGRRWREAIVPREELATYIDDRPLAGVL
                     AAEALRVAAWRPRLGFETDHRTLVHEVDWLRSAVHLNKGCYRGQEAVARVHNLGKPPR
                     RLVFLHLDGSGHVLPPRGAEVTVAGGGSGPLAGRAVGFVTSVAHHFETGPIALALVKR
                     SLPADAPLIVADGIGGPGIAASQEVIVAP"
     gene            complement(5509..>5987)
                     /locus_tag="PLK64_12125"
     CDS             complement(5509..>5987)
                     /locus_tag="PLK64_12125"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPV80539.1"
                     /translation="DGRGDRRGSGGAWAAYSGLVSDLAAVLRANGMRLTPQRRRVVDA
                     LARLGHATPDAVAAEAGADGGSPLAASTIYRALEALEELGLVAHTHLDHRAPTFHLAG
                     HADHIHLVCLGCHTIDEVPVEVAAEFVAGMAAARGFRADITHMAVHGWCSSCGGPQ"
BASE COUNT          806 a         1978 c         2261 g          942 t
ORIGIN      
        1 cgttggccgc ggtcgtggcg agcatgccgg agtgcttgcc cgaacagttc tgtgcgagtg
       61 aggttttgag gtgcccggcc cggatccatt cgtcgcgcgc ctgctcgtcg acggggtagt
      121 cgggggtgtt gagcaggtgg gcctcggtga ggccggcgcc gtgcaggatc tccagggcgg
      181 tcgcgaggtg gaacgcctca ccggagtggc tggcggcgac aagcgcgagc tggtgggggg
      241 cgacatccag gccggcggcg accatggccg cggcctggat cggcttgctc gccgaccgcg
      301 ggaagaccaa cccggtgggg tcgccccacg cctgctcgat cgacccgtcg gcacgggtga
      361 caacggcgga gcctcggtgg atgctctcga cgacaccgca ccgcaccacg tgcgcgagga
      421 cagggccctg cgtgagcgcg ggcgaaacgg ggtgtgcggc tgcgtggctg gacgggttcg
      481 acacgcgcgc cagtgtgcca gaggcaggtg ccccgcggac ccgaaggcgg cgggacggca
      541 tacccccaag accccgggta tgccggtggg ccggcttccc tgggaaaccg gcccacctct
      601 gtgcaccccc ctacggggag ttccgcctgt ggtcgaggcg gatgccgccc ccgacgacag
      661 ggttgatcag tcgccgacct tcttggcagc cgtcttggcg gcggtcgtgg tcttggcggc
      721 ggtcttgcgg acaccggtgc cggtggcgcg ggtgcgagcg gtggtgcgct tgaccccggc
      781 ctgagccgtc gtggtggtgg tgcgggccgc ggcgcgggtc cgggcggcgg aggccttgac
      841 ggtcgacgcg acggtctcgg cgtccttctc gacggtctcg gcgaccttgg cggcgacgac
      901 cttggcctct tcggcggcgg acttgatggt ggccttggcg gcgcgctcgg tggacacggc
      961 ggccttgcgg gcggtggtga cggcaccctt gccagcggca acggtggtct cgacctgcga
     1021 cttgaggtcc tgggtcgagc gggtggtgcg gatgcgctgc acgagggcct cgccgcgctc
     1081 ggcaagctcg tcgaccgtgg cctgagcctt ggtggccaac tcgatgctgt gaccgacggc
     1141 gttggccggg acggtctgga cgtccttggc cgtcttggaa agccgcttgg tcaggtcggc
     1201 ctggatcttg gcgaggttct tctggacctt cttgacgtcc ttctggaagc gctcggcgtc
     1261 cttctgcagg ctcttggcgt ccatctcgcg ggcgacctgg acgaagtgct cctgggccac
     1321 gcggatcttc tcgacggcca ggtcggtcgc gccgacgacg gccatgagtt gggtgcgggc
     1381 ctcagtggcg gtcttctgca ggtccttctt catttcgtcc atgcgggtct tgcgggccat
     1441 ggtgggtaat cctcctcagg attctggtgt ctctgtcggt tgagtcgcca cgtctccgtc
     1501 gggtgcggac cccgtggccg gggcggttct gggttcgtcg gcgatgtcca cgaaggagcg
     1561 gtagacatcg acgaggacct ggcgttggcg atcggtcagc cgaccgtcgg cggcgatcgc
     1621 ggccaggaca ctgggagcgc tcgtggggct ggactcgtcg aggatgccgg cgcggacata
     1681 gagcgactcg gccgacacct ccaagccgcg agcgatctgt tgcaggatct ccgcgctggg
     1741 cttcttgagc ccgcgttcga tctgggagag gtaggggttg gagatcccgg ccagctcgga
     1801 cagctgccgc aacgagagct tggctgcccg gcgctgttcg cgcaggtagc tgcccaagtc
     1861 gagcttgagt gtcggggtca tgccaccatt atgcttgctc tagttagcaa aacgcaaact
     1921 ggagacagcg ggccgtgcgg atgcgaggat gacgcgtatg gcgggactcc tggtggcggg
     1981 cacgagctcc gacgccggga agtcgctcat cgtcgccgga ctgtgccggg cgtgggcgcg
     2041 ccaaggcgtg cgggtcgcgc cgttcaaggc ccagaacatg agcaacaact cgatggtctg
     2101 cgccgacggc tcggagatcg ggcgcgcgca gcacctgcag gcgatcgccg ctggggtcga
     2161 gccgagcgcg ctgcacaacc ccgtgctgct caagccgtcg accgaccgcc gcgcgttcat
     2221 cgtcctgcgc gggcagccgg gcggcacgtt ggaggcgggc gagtacgtcg gcgggcggac
     2281 gcgattggct gacgcggcat acgaggcgta tgccgtgctc gccgcgacgc acgacctggt
     2341 cgtctgcgag ggggccgggt cgccggcgga gatcaacctg cgggcgggcg actacgtcaa
     2401 tttcggtctg gcgcaacggt tcgggctgcc cgtcgtgctc gtcggcgaca tcgatcgcgg
     2461 tggcgttctg gcgtcgatct tcggccactg gggcatcgtc gacgacgccg atcgggcgct
     2521 cctggcgggg tatgtcatca acaagttccg cggcgaccag tcggtgctgg acccggggct
     2581 ggacgagctg acccggcgaa ccgggttgcg gtgcttcgga gttgtcccgt ggctcgacca
     2641 ggtgtggctg gactccgagg atgcgttggc cttcgccaag tggccgaggc tgcctgggcg
     2701 ggccgggacg ttgcgggttg cggcgatccg cctcccgcgg atctcgaatg ccacggacct
     2761 ggatgccatc gcgtcggagc cgggggtcga tgtgttcgcc accgccgagc ccgccgacat
     2821 cgagcatgcg gacctcgtcg tcctgccggg gagtcgggcc acggtggccg atctggagtg
     2881 gctgcggcgg cgcgggctgg ccgatgccct ggctcaacgg gcggctgcgg ggcggccgat
     2941 cctcgggatc tgcgggggtt atcagatgct cacccacgag atcgacgacc cggtcgagtc
     3001 gccgagcggg ggttgtgccg ggctcgggct gctgccggcg cgagtgcggt tccagccgga
     3061 gaaggtgttg gggcggccgc ggggcaggtg gcgtggccat gacgtggtcg cctacgagat
     3121 ccatcacggg gtggttgagc cgttggcggg ttctggcggc gagggcggtt tgggcggtga
     3181 gcccttggcg ggttctcgtg gcgagcgcag cgagaggggc gcggggagcg agccgttcct
     3241 ggacggcata cgcgatggcg ctgtgtgggg cacgatctgg cacggggcgt tcgagaacga
     3301 cggcttccgg cgcgcgtggc tcgccgagat cgccgccgcg gtggggagca cgtgggtgcc
     3361 gcgcaccgac gcgccggggt tcagcgagcg gcgcgagcgg atgctcgatg acctggccga
     3421 tgcgttggag gcgtgcgtcg atctcccggc cctgttggcc gcggccaggg gcgcgacagg
     3481 cccgtgactc agcgggctcg gcggtccttg atcccgagga gctcgcgcgc ctgggtggtc
     3541 gagaaggccg gccgctgcat cagcccggcc agcgtggccg cccgctcgac gaactcgacg
     3601 ttgtgctggg cgacctgacc gcgggcgacg aaaaggttgt cctccatgcc gacccgcaga
     3661 tgcccaccgg cggcgagcgc ggcgccgagg atcggcaggt gggcccgacc gatgccggtc
     3721 gccgaccaac tggtcacctc ggcgggcagc atcgcgacgc cggccagcag cgcctcgacg
     3781 gtgccgggca ttgcgccggg cacccccgtg acgaagtcga cgtggacctt gccgccgaac
     3841 ggcaacccgc acgtgtcgat cagccggcgc agcgcgtgga catgcccgag ctcgaagagc
     3901 tcgaactccg gcaccacctc acgctcctgg gccagccgat agaggtcgac catgaagccc
     3961 catgggttga ggaagatgtc gtcgccgaag ttggtcgtcc cgcaggtcag cgagcacgag
     4021 tccgggtcag cgtcgagcac ggtgaggcgc ttctcgagtg ggtcgtgcac ggagccgccg
     4081 gtcgagagct gcacgacgag gtcggtcgcc tcccgcacgg ccacaaccgc gtcccgcagg
     4141 tatgccgcgt cgagagtcgg gcggtgctcg cggtcccgca cgtggatgtg gatcaggcca
     4201 gcgcccgcgg cctcgcagcg ccgggccgtc tcgacgagct cgtcgagcgt ggtgggcagt
     4261 tggggcacgt cggccttggc cgtctcggcc ccggtgggag caacggtgat gagagtgcct
     4321 acagcgctgg aagcggacat catgctgtca ttcttccatt ttgccgaaga tttttcacct
     4381 tgtcacgcct cctgccggat acttgggcgc tgcggcgtgc ctacggggcg acgatgacct
     4441 cttgcgaggc ggcgatcccc ggccccccga tgccatcggc cacgatgagc ggggcatcgg
     4501 ccgggagcga gcgcttgacc agtgccaacg cgatcgggcc agtctcgaaa tggtgcgcca
     4561 ccgaggtcac gaaccccacc gctcggccgg ccagggggcc gctcccccca ccggcgaccg
     4621 tcacctcggc gccgcgcggc ggcagcacat gccctgatcc gtccaggtgc aggaacacca
     4681 gtcggcgcgg tggcttgccg aggttgtgca cgcgcgcgac cgcctcctgc ccgcggtaac
     4741 accccttgtt caggtgcacc gccgagcgca gccagtcgac ctcgtgcacg agcgtccggt
     4801 gatcggtctc gaaccccagc ctcggccgcc atgccgcaac ccgcagcgcc tcggcggcca
     4861 gcactccggc cagcggccgg tcgtcgatgt aggtcgccaa ctcctcgcga ggcacgatcg
     4921 cctcgcgcca gcgtcgccct tgcgcggggt gaccgtcgac tggtccgtat gccgcgatcc
     4981 gccccgcccg cccggacggc gtcagatcga tgacccccgg ccatgggtcg acccacacga
     5041 ccggctcacc gggcgcagcc acagccccga aaggttcgcc gatgacggca taccgatcgg
     5101 tgacgtcggc gacctcgacg cggagcatga agcgcatccg ctccaaccag gccaccagcg
     5161 cgggcgcggt gccgggctcg acggtgatcc acgtggtcgt gccgtcatcg acgaggtgca
     5221 gcgcatgctc gacgtgcccg tgcgggtcca ggacgagcga ctctgtgccg accccaggct
     5281 cgatcccggt cagcgcctgg gtcgtcatcg agttcaacca cgagagtcgg tcgggcccgg
     5341 tcaccgtgac gacgccccgg ttggacaggt cgaccgcggc taggccctcg gtgagcaacc
     5401 gctgctcgcg catcgggtcg ccgtagtgcc cggcgacccc ggcatcgact ccctcggccg
     5461 ccaccgcacc gggggcgtcg agaaggggtg aggggacggc atgcccggtc attgcggacc
     5521 tccgcacgag gagcaccagc cgtgaacggc catgtgcgtg atgtcggcgc ggaacccccg
     5581 cgccgcggcc atccccgcga cgaactccgc cgcgacctcg accggcacct cgtcgatcgt
     5641 gtgacagccg aggcagacga ggtggatgtg atcggcgtgc ccggccaagt ggaacgtggg
     5701 ggcgcgatgg tccaggtggg tgtgggcgac gaggccaagc tcctcgagcg cttcgagcgc
     5761 gcggtagatg gtcgaggcag cgagggggga gccgccgtcg gcgcccgctt cggcggcaac
     5821 cgcgtcgggg gtcgcgtgcc cgagccgggc gagcgcgtca acgacgcggc gccgttgggg
     5881 cgtcagccgc atcccgttgg ctcgcaacac cgctgcaagg tcactcacca gcccactgta
     5941 tgccgcccac gcgccgcccg atcccctgcg atcccctcgc ccgtcgc
//
LOCUS       DAOMZY010000226         5979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_12480_length_5979_cov_3.623734, whole genome shotgun sequence.
ACCESSION   DAOMZY010000226 DAOMZY010000000
VERSION     DAOMZY010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5979
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12480_length_5979_cov_3.623734"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PLK64_12130"
     CDS             complement(<1..657)
                     /locus_tag="PLK64_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HPV80540.1"
                     /translation="MSVLASQRRSAILALVEEAGAARVSELVDQLGVSDMTVRRDIER
                     LDAEGLLERVHGGAIALLPRAADEPGFNAKSSLMTAEKQAIAQAAAALVEPGATIGIS
                     AGTTTYEFARAIRNMPALTVVTNSVPVAQLLHEGSGNHVVVLTGGVRTPSDALVGPVA
                     VSALKGLHVDRLFLGAHGIDRNAGLTTPNLVEAETNRALVGASRSVCVLADHTKVGIV
                     G"
     gene            complement(654..2642)
                     /locus_tag="PLK64_12135"
     CDS             complement(654..2642)
                     /locus_tag="PLK64_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HPV80541.1"
                     /translation="MRQWPENRIAFGGDYNPEQWPREVWADDLRLMAEAGVSFVTLGV
                     FSWSWLEPSKGEYTFDWLDDIMDLLGEHGIAVDLATATATPPPWLTSAYPEILPVDRE
                     GHTLWPGSRQSWCPSSPLYREFALALTERLASRYHDHPALAMWHVSNEYACHNLPCYC
                     DTCAVAFRRWLEQRHGTLDALNDAWGTAFWSQRYTSWDDILPPRITTTFSNPTHVLDY
                     SRFGSDTLLDFYRSEYEVIRRHSPTVPITTNFMTMSQFRLLDYHDWAPHQDVVSTDHY
                     VVNSLEHPQAELSFHGDLTRGLAGGRPWMLMEHSTSAVNWQPVNPAKVPGGTILDSLA
                     HVARGADTLGFFQWRQSRSGSEKFHSALVPHAGEDSDRFREVCELGAIADRLGEVLGS
                     RVDADVAVLWDYEALWAMSGPCMPSSAIDYATAGHTIHRLLRDRGVTCDVVHPSADLS
                     RYRVVVVPTLYLVSDEDAASIAAAAAAGAHVVVTYFSGISDANDHVRLGGYPGAFRDL
                     LGVRVEEFFPLREQEGVALSDGGTGSQWSEDARATDAQVVTTYANGPLAGRPAVTRRD
                     VDAGVAWYLGTLPDDASLGSLLDTVLHESGVESVVRTSPGVEVVRRRSDSGSWLFVLN
                     HTDDACQVTASGHDLVSSTDVGPTLHLSPRTAAVVREG"
     gene            2906..4258
                     /locus_tag="PLK64_12140"
     CDS             2906..4258
                     /locus_tag="PLK64_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPV80542.1"
                     /translation="MARPTSREAQFSSTLPASVRGVDRRSLLRGAIGAGALFGGASLL
                     GACGSDPAPVASGGASAKAGPVSFGINEAKGSGPGYERLIGMADAYTKNTGAAVTTNA
                     VDHNTFQESINTYLQGTPDDVFTWFAGYRMAQFAENGLISDISDIWPIDGLSDAFKKS
                     STAPDGKQYFVPVQYYPWAVFYRKSVFEKNGWTAPATKADFDALLKDMQGKNITPFAF
                     AAKDAWAPMGTFDVLNMRLNGYDFHMELCRGKQKWDSPEVRNVFATWKTLLPYHQENS
                     LGRTWQEAATSMANGECGMYLLGTFVVDGVPPEAAADLDFFTFPALDDTIGNDALDAP
                     IDGFCVSGSSENQEPAKAFAKWLGSAEAADAANNNASAPFIAANSGASTEKYGELQKK
                     SAELVQNAKSIAQFLDRDTNADLANTVITASLQEFIKNPDNIDSITTSIQQQAASILG
                     "
     gene            4284..5204
                     /locus_tag="PLK64_12145"
     CDS             4284..5204
                     /locus_tag="PLK64_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018774003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPV80543.1"
                     /translation="MAITTTKRRSKGSTRRVEKRQSTRDRLVVMAMVGVPTLFVIWLV
                     WLPALASVALSFTRWNGFKLSGIEWVGTQNYKDIVTIYPPFWPAIQNNLIWLVFLFIL
                     PTILGMLIAVVLDREMRGSRFYQTAFYLPVVLSMALVGFIWQLFYSADQGLLNAVLGT
                     RIDWYGDPDINLWSVLVATSWRHTGYIMVIYLAGLKGVDASLREAAAVDGAGEVKTFF
                     HVILPVMRPINLIVLVIVVIESLRAFDLVWIINKGRNGLELISALVSQNVIGEATRYG
                     FGSALAVIMMLISSVFITIYLRIVFQEERR"
     gene            5201..>5979
                     /locus_tag="PLK64_12150"
     CDS             5201..>5979
                     /locus_tag="PLK64_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPV80544.1"
                     /translation="MSATTTSATTTGAAKPSVHTGAGGVMNKRRGTIATIVMLILALL
                     WLFPLLWALYNSFRSYSYTQTNGYLSFGGWTLENYQNAWDRGNFGLHLKNSLLITIPA
                     VSLTLFLSSMVAFVLARFSYRFNLTLLGVFLAANLLPPQALLIPVFKMFREIPLPTFM
                     SEGGTMLNSFWALILVNTAFQMGFCTFVLSNYMKTLPYEIYESAQLDGASVWRQYWQL
                     TMPLVRPAMAALATLQVTWIYNEFFWATVLISKGDKLPVTSA"
BASE COUNT          974 a         2032 c         2014 g          959 t
ORIGIN      
        1 gccgacgatc cccaccttgg tgtggtccgc cagcacgcac accgagcggc tggcgccgac
       61 gagggcgcgg ttggtctcgg cctcgacgag gttgggggtg gtgagcccgg cgttgcggtc
      121 gatgccgtgg gcaccgagga agaggcggtc gacgtgcagg cccttcagcg cggacacggc
      181 gaccggcccg acgagggcgt cggacggggt gcgcacccca ccggtgagca cgaccacgtg
      241 gttgccgctg ccctcgtgga gcagctgggc gaccggcacg gagttcgtca ccaccgtgag
      301 ggcgggcatg ttgcggatgg cccgggcgaa ctcgtacgtc gtcgtcccgg cggagatccc
      361 gatggtggcc ccgggctcga cgagggcggc cgcggcctgg gcgatcgcct gcttctccgc
      421 ggtcatcagc gaggacttgg cgttgaagcc gggctcgtcg gcggcccgcg gcagcagggc
      481 gatggcgcca ccgtgcaccc gctcgagcag tccctcggcg tcgaggcgct cgatgtcgcg
      541 gcgcacggtc atgtccgaca cgccgagctg gtccacgagc tcggacaccc gcgcggcgcc
      601 cgcttcctcg acgagcgcga ggatggcgga acggcgctgg ctggcgagca cgctcacccc
      661 tcccgcacca cggccgcggt gcgcggcgac aggtggagtg tcggcccgac gtcggtgctc
      721 gacacgaggt cgtggccgga tgccgtgacc tggcatgcgt cgtcggtgtg gttcagcacg
      781 aacaaccagc tgccggagtc ggatcggcgt cgcacgacct ccactcccgg cgaggtgcgc
      841 accaccgact cgacgcccga ctcgtgcagg acggtgtcca gcagggagcc gagggaggcg
      901 tcgtcgggga gggtgcccag gtaccaggcg accccggcgt cgacgtctcg acgggtcacg
      961 gccggacgtc cggcgagggg cccgttcgcg taggtcgtga cgacctgcgc gtcggtggcc
     1021 ctggcgtcct cgctccactg cgagccggtg ccgccgtccg agagcgcgac gccctcctgc
     1081 tccctcagcg ggaagaactc ctcgacgcgc acgccgagca ggtcacggaa cgcccccggg
     1141 tagcccccga gccggacgtg gtcgttggcg tcgctgatcc cggagaagta ggtcacgacc
     1201 acgtgcgcgc cggcggcggc agcggcggcg atcgacgcgg catcctcgtc ggacacgagg
     1261 tagagcgtcg ggacgacgac gacgcggtag cgcgacaggt cggccgaggg gtgcacgacg
     1321 tcacaggtga ctccgcggtc gcgcagcagc cggtggatgg tgtgccccgc ggtggcgtag
     1381 tcgatggcgc tgctcggcat gcacgggcca ctcatggccc acagggcctc gtagtcccag
     1441 aggaccgcga cgtcggcatc gacacggctg ccgaggacct cgccgagccg gtcggcgatc
     1501 gccccgagct cgcacacctc gcggaagcgg tcgctgtcct caccggcgtg ggggaccagg
     1561 gccgagtgga acttctccga gcccgagcgc gactggcgcc actggaagaa ccccagggtg
     1621 tcggcgccgc gggccacgtg ggcgagggag tccaggatcg tccctcccgg caccttggcg
     1681 gggttgacgg gctgccagtt gaccgcgctc gtcgagtgct ccatgagcat ccacgggcga
     1741 ccgccggcca gcccgcgggt gaggtcgccg tggaaggaca gctcggcctg ggggtgctcg
     1801 aggctgttga cgacgtagtg gtcggtgctc acgacgtcct ggtgcggcgc ccagtcgtgg
     1861 tagtcgagga gccggaactg gctcatcgtc atgaagttcg tcgtgatcgg cacggtgggg
     1921 gagtgccggc ggatgacctc gtactcgctg cggtagaagt cgagcagggt gtcggagccg
     1981 aagcgcgagt agtcgagcac gtgggtcggg ttcgagaacg tcgtcgtgat gcgcggcggg
     2041 aggatgtcgt cccacgaggt gtagcgctgg ctccagaagg ccgtgcccca ggcgtcgttg
     2101 agggcgtcga gggtgccgtg gcgctgctcg agccagcgcc ggaaggcgac cgcgcaggtg
     2161 tcgcagtaac acggcaggtt gtggcaggcg tactcgttgg agacgtgcca catcgcgagc
     2221 gccgggtggt cgtggtatcg cgaggcgagg cgctcggtga gggccagggc gaactcccgg
     2281 tacagcggcg aggacggaca ccaggactgg cggctgcccg gccagagggt gtggccctcg
     2341 cggtcgacgg ggaggatctc ggggtaggcc gaggtgagcc agggcggggg ggtcgcggtc
     2401 gccgtggcga ggtcgacggc gatgccgtgc tcgccgagca ggtccatgat gtcgtcgagc
     2461 cagtcgaagg tgtactcacc cttgctcggc tccagccacg accacgagaa cacgccgagc
     2521 gtgacgaagc tgacgccggc ttccgccatc aggcgcaggt cgtcggccca cacttcccgg
     2581 ggccactgct cagggttgta gtcgccgccg aaggcgattc ggttctcggg ccactgacgc
     2641 atggtggcca gtctgtgacc ttgaacaaac atctgtcaac aactatcaac agccaatctc
     2701 cctcgactcg gcgaaccgcc ctcggtgccc ggagcggccc gattcctgta cgcaacgggc
     2761 gatttgttgc acggtgggcg cgacgatcgt gtcgaatccg taaccaacac cccttgacag
     2821 cggctgcctc tggccagaac actcaggttc gtagatgttc gtttctgatc gaagttgtca
     2881 gaacagcccc gtcaaaggag acaccatggc acgcccgacc tcgcgtgagg cacagttctc
     2941 gagcaccctc cccgcctccg tgcgaggcgt cgaccggcgc agcctgctgc gcggcgccat
     3001 cggcgcgggg gccctcttcg gaggagcttc gctcctgggt gcctgcggca gcgaccccgc
     3061 cccggtggca tccggcgggg cttccgccaa ggccggcccg gtgtccttcg gcatcaacga
     3121 ggccaagggt tcgggcccgg gctacgagcg cctcatcggc atggccgacg cctacacgaa
     3181 gaacacgggc gcggccgtca cgaccaacgc cgttgaccac aacaccttcc aggagagcat
     3241 caacacctac ctgcagggca cgcccgacga cgtcttcacc tggtttgccg gctaccgcat
     3301 ggcgcagttc gccgagaacg gcctcatcag cgacatcagc gacatctggc cgatcgacgg
     3361 cctcagcgac gcgttcaaga agtcctcgac tgcccccgac ggcaagcagt acttcgtgcc
     3421 ggtccagtac tacccgtggg ccgtgttcta ccggaagtcg gtcttcgaga agaacggctg
     3481 gaccgccccc gcgaccaagg ccgacttcga cgccctcctg aaggacatgc agggcaagaa
     3541 catcaccccg ttcgccttcg ccgccaagga cgcctgggcg ccgatgggca ccttcgacgt
     3601 gctcaacatg cgcctgaacg gctacgactt ccacatggag ctgtgccggg gcaagcagaa
     3661 gtgggacagc cccgaggtgc gcaacgtgtt cgccacgtgg aagaccctgc tgccctacca
     3721 ccaggagaac tcgctcggcc gcacctggca ggaggccgcc acctcgatgg ccaacggtga
     3781 gtgcgggatg tacctgctcg gcaccttcgt cgtcgacggc gtgccgccgg aggccgcagc
     3841 cgacctcgac ttcttcacct tcccggcgct cgacgacacc atcggcaacg acgccctcga
     3901 cgcacccatc gacgggttct gcgtctcggg cagcagtgag aaccaggagc cggccaaggc
     3961 cttcgcgaag tggctcggct cggccgaggc ggccgacgcg gcgaacaaca acgcgtccgc
     4021 gccgttcatc gccgccaact ccggtgccag caccgagaag tacggcgagc tgcagaagaa
     4081 gtcggccgag ctggtccaga acgcgaagtc gatcgcccag ttcctcgacc gcgacaccaa
     4141 cgccgacctg gcgaacaccg tcatcaccgc atcgctgcag gagttcatca agaaccccga
     4201 caacatcgac tcgatcacta cgtccatcca gcagcaggcc gcgtccatcc tcggctgacg
     4261 gacgaagcga ggagaggagc cccatggcca tcaccacgac aaaacgacgc agcaagggca
     4321 gcacgcgccg cgtcgagaag cgacagagca cacgcgaccg cctggtcgtc atggcgatgg
     4381 tcggggttcc caccctgttc gtcatctggt tggtctggct cccggcgctc gcgtcggtgg
     4441 ccctgtcctt cacgaggtgg aacggcttca agctgagcgg catcgagtgg gtcggtaccc
     4501 agaactacaa ggacatcgtc accatctacc cgccgttctg gcccgccatc cagaacaacc
     4561 tcatctggtt ggtcttcctg ttcatcctgc cgacgatcct cgggatgctc atcgcggtcg
     4621 tcctcgaccg cgagatgcgc gggagccggt tctaccagac tgcgttctac ctgccggtgg
     4681 tcctgtcgat ggccctcgtg ggcttcatct ggcagctgtt ctactccgcc gaccagggcc
     4741 tgctcaacgc cgtgctcggc acgagaatcg actggtacgg cgaccccgac atcaatttgt
     4801 ggtcggtgct cgtcgccacc tcgtggcggc acaccggcta catcatggtc atctacctgg
     4861 cgggcctgaa gggcgtcgac gcgtcgctgc gtgaggcagc ggccgtcgac ggggccggcg
     4921 aggtcaagac gttcttccac gtcatccttc cggtgatgcg gccgatcaac ctgatcgtcc
     4981 tggtcatcgt cgtcatcgag tcgctgcgag cgttcgacct cgtctggatc atcaacaagg
     5041 gccgcaacgg gctcgagctc atctcggccc tggtgtccca gaacgtcatc ggcgaggcca
     5101 cccggtatgg cttcggttcg gcgctcgccg tgatcatgat gctcatctcg tcggtcttca
     5161 tcaccatcta cctgcgcatc gtcttccagg aggagcgccg atgagcgcca cgaccacctc
     5221 agccacgacc acgggcgcgg ccaagccgtc cgtccacacg ggcgcgggcg gggtgatgaa
     5281 caagcgccgc ggcaccatcg cgacgatcgt catgctcatc ctcgcgctgc tgtggctctt
     5341 ccccttgctg tgggccctgt acaactcctt ccgcagttac agctacacgc agaccaacgg
     5401 ctacctctcc ttcggcggct ggacgctgga gaactaccag aacgcctggg accgcggcaa
     5461 cttcggtctg cacctgaaga actcgctgct catcaccatc ccggcggtgt ccttgacgct
     5521 cttcctgtcc tcgatggtcg cgttcgtcct ggcgcggttc tcctaccgct tcaacctcac
     5581 cctgctcggg gtcttcctgg cggccaacct gctgcccccg caggcgctgc tcatccccgt
     5641 gttcaagatg ttccgcgaga tcccgctgcc gacgttcatg agcgagggcg gcacgatgct
     5701 gaacagcttc tgggcgctga tcctggtcaa caccgccttc cagatggggt tctgcacgtt
     5761 cgtgctgagc aactacatga agacgctgcc gtacgagatc tacgagtccg ctcagctcga
     5821 cggcgcgagc gtctggcgcc agtactggca gttgaccatg cctctggtgc gaccggcgat
     5881 ggcggccctg gctaccttgc aggtcacctg gatctacaac gagttcttct gggcgaccgt
     5941 tctcatctcc aagggtgaca agctgcccgt cacgagcgc
//
LOCUS       DAOMZY010000227         5953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_12566_length_5953_cov_5.747372, whole genome shotgun sequence.
ACCESSION   DAOMZY010000227 DAOMZY010000000
VERSION     DAOMZY010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12566_length_5953_cov_5.747372"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="PLK64_12155"
     CDS             complement(<1..847)
                     /locus_tag="PLK64_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HPV80545.1"
                     /translation="MSTIPTPAVSRALARAVRGWLDHLDVERGVARNTLVSYTRDLDR
                     YLDYLARQGVSTPENVTEAHVSGFLADLRAGSPGRMPLSASSAARTLVAVRGLHRFLA
                     LEGRTLADPAEGVRPPKTPLRLPKAIGVDDVERLLAAASLGDTPVSMRDRALFEVLYG
                     AGARISEAVGLDVDDLDLESGTVRLFGKGSKERIVPVGRYAREAVAAYLVRGRPALAA
                     RGKGTPALFLGTRGARLSRQTAWSALAAAGERAGLAGHLSPHTLRHSFATHLLEGGAD
                     VRVVQE"
     gene            complement(844..2694)
                     /locus_tag="PLK64_12160"
     CDS             complement(844..2694)
                     /locus_tag="PLK64_12160"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPV80546.1"
                     /translation="MGTLITTLSSAAPRMTPGERRLAQRLQQKLDADYLVWFDVPVGP
                     MRTHPDFVVLHPSRGVLILEVKDWALPLIQRVDREMWDIQPDGRHKRVINPILQARHG
                     AHDVVRALERDLQLVHEAGAHQGKLAFPWGYGVVFTKIDRAEFDAAGLGEVIDGHAVM
                     CADEMTESVDPEAFQERLWGMFPRRFPAKLTLPQIDRVRWIMFPDVRVDAPVASLFDA
                     AASPDSPEGDAGAAAAIELPDVMKVMDLQQEQLARSLGAGHRVVHGVAGSGKTMILGY
                     RAEYLARLVAPGAKPVLVLCFNEPLGAALSANFASRGLADRVQVRHFHRWARQQLVTF
                     GQPLPPDGPHFFDDLVGYLIRAVDRGHVPRAQYSAVLIDEGHDFRPEWLHLVVQMVDP
                     ETNSLLLLYDDAQNIYGRGRTPAFSFKSVGIQAVGRTTILRVNYRNTTQILRLAQRVA
                     GDILKPQACDEDGAPLLGALGCGRDGEEPDVRRLPTLGAEAAYVAQRFAAAARDGRAW
                     SDMAVLCHDTAVMDACASALRRVRIPHSVRRRPGDFRPEADTVKILTMRVSKGLEFPV
                     VAIPGVGRLPAPHEDDKDAARLFYVAATRATERLLVTVSGDGSLGQRLGV"
     gene            2804..3412
                     /locus_tag="PLK64_12165"
     CDS             2804..3412
                     /locus_tag="PLK64_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80547.1"
                     /translation="MKLYADLPGRRAAQVVADVLSVGWILACTNIGKAVHDATMGLRQ
                     PADGLASAGNQLEAGMTSAGDRIAQVPLVGDALQGPFRDAAGTGVALAQVGTDLSGSI
                     GRVALIAGIVVAILPILLWMLVWLPLRIRFIRRASDAANFIEGRADLDLFALRALVRV
                     PTKRLATIHADPAAAWRSRDPHVIRELANLELLQCGVRPPRA"
     gene            complement(3444..4793)
                     /gene="hisS"
                     /locus_tag="PLK64_12170"
     CDS             complement(3444..4793)
                     /gene="hisS"
                     /locus_tag="PLK64_12170"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPV80548.1"
                     /translation="MSTGKVTPISGFLEFLPEQRIVEQHFLDVIREVFELHGFASIET
                     RAVESVERLLGKGGDADKEIYGISRLAADASDSREPSLGLHFDLTVPFARYVLENAGK
                     LAFPFRRYQMQKSWRGERPQEGRYREFTQCDIDVVDVGSLSPQYEIEMPLVLTDVFDR
                     LPIGDYVVQVNDRRIPQGFYRGLGIEDVGGTLRIVDKLDKLPADTVRTMLLDSGCTAE
                     QADQCLALAGIRTEDLSFIEQVRALGVADPLIDEGLTGLAAVVDAGRTHAPGRVVADL
                     KLARGLDYYTGTVYEVRLLGFESWGAVAGGGRYDALATDGTVTYPGVGISIGVSRLLG
                     LLFRQGLVSASRSTPACVLVALTDEESRPRSLAVASALRGRGIPCEVAPRPDKFGKQI
                     RYAARRGIPYVWFPGGPESDGDSVKDIRSGEQVPATAVDWAPPAADLRPSVVRAAAD"
     gene            complement(4896..5591)
                     /locus_tag="PLK64_12175"
     CDS             complement(4896..5591)
                     /locus_tag="PLK64_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPV80549.1"
                     /translation="MLMIGFPAAAFDTNCFVLATAEGEECVVVDPGIGVTDTLSEVLR
                     ELRLRPAAVLLTHGHLDHVYSVTPVCRGGGAAAYIHGDDRYRLRDPLADLDRGMIAMM
                     EAQFGERATWTEPDDIRELRDGDSVTIAGMAFGIVHAPGHTEGSVMFTLADVPEGINV
                     EVGSTVISGDVLFAGSIGRSDLAGGDPAAMNRSLRDCVLPMPDDRLVLPGHGPLTTIG
                     RERASNPYLRGLR"
     gene            5726..>5953
                     /locus_tag="PLK64_12180"
     CDS             5726..>5953
                     /locus_tag="PLK64_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80550.1"
                     /translation="MRDKQPVLAVIGVLLAVIAFVWVANVVTAPKVADPTATGEPTST
                     ATTTATDNPSATATATPTPTPTPKVLAGCTEV"
BASE COUNT          934 a         2084 c         2060 g          875 t
ORIGIN      
        1 actcctgcac gactcggacg tcggcgccgc cttcgagcag gtgggtggcg aacgagtgcc
       61 gcagcgtgtg cggcgacaag tggccggcca ggcccgcccg ctcccctgcc gctgccagcg
      121 ccgaccacgc ggtctggcgg ctgagccgcg caccccgggt gccgaggaac agggccggag
      181 tgcctttccc gcgggccgct agcgcaggtc gcccccgcac gaggtatgcc gccaccgcct
      241 cccgtgcgta gcgccctacg gggacgatcc gctccttgga ccccttgccg aagagccgaa
      301 cggtgcccga ctccaggtcg agatcatcga cgtcgagccc gacggcctcc gagatccgcg
      361 ccccggcgcc atagaggacc tcgaacaagg cccggtcgcg catcgagacg ggggtgtccc
      421 ccagtgacgc ggcggcaagc agccgttcga cgtcgtcgac accgatggct ttaggcaacc
      481 gcagcggggt cttgggcggt cgcacgcctt cggccggatc ggccagggtg cgcccctcca
      541 acgcgaggaa ccgatgcagc ccccgtactg cgacgagggt gcgagccgcg gacgaggccg
      601 agagcggcat acgccccggt gagcccgctc gcaggtcggc gaggaagccg gacacgtgtg
      661 cctcggtgac gttttcaggc gtggaaacgc cttggcgcgc aaggtaatcc aggtagcggt
      721 cgaggtcgcg ggtgtaggag accagggtgt tgcgggcgac tccccgctcg acgtcgaggt
      781 ggtcgagcca ccctcgcacc gcgcgcgcca gcgcccgcga gacggctggc gttgggatcg
      841 tgctcatact ccgagccgtt ggcccaggga gccgtcgccg ctgaccgtga cgagcagccg
      901 ctcggtcgcc cgggtggcgg cgacgtagaa cagccgcgcc gcgtccttgt cgtcctcgtg
      961 aggggctggc aggcggccca ccccagggat cgccacgacg gggaactcca gccccttgct
     1021 cacgcgcatg gtgaggatct tgaccgtgtc ggcctcagga cggaagtcgc cgggccgacg
     1081 tcgcaccgaa tgcgggatcc gaacccgccg tagcgccgac gcacacgcgt ccatgacagc
     1141 ggtgtcgtgg cacagcacgg ccatgtccga ccaggcccgt ccgtcccgcg ccgccgcggc
     1201 aaacctctgg gcgacgtatg ccgcctccgc accgagcgtg ggcagccgcc gaacgtcggg
     1261 ctcctccccg tcccgcccgc acccgagggc gccgagaagc ggggcgccgt cctcgtcgca
     1321 tgcctgtggc ttgaggatgt cgcccgccac ccgttgggcc aggcgcagga tctgcgtggt
     1381 gttgcggtag ttgacccgaa ggatcgtcgt ccggccgacc gcctgaattc ccacgctctt
     1441 gaagctgaac gccggggtgc gcccccggcc gtagatgttc tgggcgtcgt cgtagaggag
     1501 cagcaggctg ttggtctcgg ggtcgaccat ctgcacgacg aggtgcagcc actcggggcg
     1561 gaagtcatgg ccttcgtcga tgaggacggc ggagtattgc gccctcggca cgtggccacg
     1621 gtcgacggcc cggatgaggt agccgacgag gtcgtcgaag aagtgtggtc cgtcgggtgg
     1681 cagcggctga ccgaaggtga ccagttgttg gcgggcccag cggtggaagt gtctgacctg
     1741 cacgcgatcg gccaggcccc gcgaggcgaa gttggccgac agcgcggcac cgagcggctc
     1801 gttgaagcac aacaccagca cgggcttggc gccaggggcg acgaggcggg caaggtattc
     1861 ggctcggtag cccaggatca tcgtcttgcc cgacccggcc accccgtgga ccacgcggtg
     1921 cccggccccg aggctgcgtg ccaactgctc ctgctgcagg tccatgacct tcatgacgtc
     1981 gggcagttcg atggccgcgg ccgcgcctgc gtccccctcg ggcgagtcgg ggcttgccgc
     2041 ggcgtcgaag agcgaagcaa caggtgcgtc cacccgcacg tcgggaaaca tgatccaccg
     2101 cacccgatcg atctgcggca gcgtgagttt cgccgggaag cgccgaggga acatccccca
     2161 caaccgctcc tggaacgcct cagggtcgac agattcggtc atctcgtcgg cgcacatgac
     2221 cgcgtgcccg tcgatcacct cgcccagccc ggccgcgtcg aactccgcgc ggtcgatctt
     2281 ggtgaagacc acgccgtagc cccacgggaa ggcgagcttc ccctgatgcg ctccggcctc
     2341 gtggaccaac tgaaggtcgc gctccagagc ccgcacgacg tcgtgagccc cgtgtctggc
     2401 ctgcaggatc gggttgatca cccgcttgtg tcgcccgtcc ggctggatgt cccacatctc
     2461 ccgatcgacc cgttggatga ggggcagagc ccagtccttg acctcaagga tgagcacccc
     2521 tcgactcggg tgcaggacaa cgaaatcggg gtgggtgcgc atcgggccga ccggcacgtc
     2581 gaaccacacg agatagtccg cgtcgagctt ctgctgcaag cgttgggcca gccgacgctc
     2641 gcccggtgtc atccgcgggg cagcgctgct caacgtcgtg atcaacgtgc ccatgctcgg
     2701 caggctagtc cgcgtggacg gcgacgcaac cgcaggcgac caccagagcg acggcgtagc
     2761 gtgagcctga cactgcctgc gttgccctgg tgaggagatc cccatgaagc tgtatgccga
     2821 tctgcctggg cgccgcgccg cccaggtggt cgctgacgtc ctcagcgtgg gctggatcct
     2881 cgcctgcaca aatatcggga aggccgtcca cgacgcgacc atgggcctgc gccaacccgc
     2941 cgacggactg gcgtcggccg ggaaccagtt ggaggcgggc atgacctctg cgggcgaccg
     3001 gatcgcccag gttccgctcg tcggcgatgc cctccaagga cccttccggg atgccgccgg
     3061 caccggtgtc gcgctggccc aggtcgggac cgatctcagc ggctcgatcg gtcgcgtggc
     3121 cctgatcgcc ggaatcgtgg tggcgatcct tccgatcctg ctgtggatgc tggtgtggct
     3181 gccccttcgc atccggttca tccgccgggc cagcgatgca gccaacttca tcgagggtcg
     3241 ggccgacctg gacctgttcg ccctgcgagc actggtgcgg gtgcccacga agcggttggc
     3301 cacgattcac gccgatcctg ccgctgcgtg gcgatccagg gacccccacg tgatccgtga
     3361 gttggccaac ctcgagttgc tccagtgcgg agttcgccca ccccgcgcct gatcaggcgc
     3421 tgaccggctg tcgcgttggt cggtcagtcc gccgcggcgc gcaccacgga agggcgcaga
     3481 tccgccgccg gaggtgccca gtccaccgcc gtcgccggga cctgctcgcc cgagcggatg
     3541 tccttgaccg agtcgccgtc cgactccgga ccaccgggga accagacgta ggggatgccg
     3601 cggcgcgcgg cataccggat ctgcttgccg aacttgtcgg ggcgcggcgc gacctcgcac
     3661 ggtatgccgc gtccgcgcag tgccgaggcg acggccaacg accggggccg ggactcctcg
     3721 tcggtgaggg cgaccaggac gcaggccggg gtcgaccggg aggcgctcac caagccctga
     3781 cggaacagca ggccgagcag tcgggagacg ccgatggaga tgccgacacc cggataggtc
     3841 acggtgccgt cggtggccag cgcgtcgtag cgccctcccc cggcgacggc tccccaggac
     3901 tcgaagccaa gcaggcgcac ctcgtagacc gtgccggtgt aatagtccag gccacgggcg
     3961 agcttgaggt cggcgacgac cctcccgggg gcgtgggtgc gcccggcgtc cacgacggcc
     4021 gccaacccag tcagtccctc gtcgatgagc gggtcggcca cgccgagcgc ccggacctgc
     4081 tcgatgaagg acagatcctc ggtgcggatc ccggccagcg cgaggcactg atcggcctgc
     4141 tcggcggtgc agccggagtc gagcaacatc gtccggaccg tgtccgcagg gagcttgtcg
     4201 agcttgtcga cgatccgcag ggtgcccccg acgtcctcga tgcccaggcc tcggtagaac
     4261 ccctgcggga tccgccggtc gttgacctgc acgacgtagt cgccgatcgg cagccggtcg
     4321 aagacgtcgg tgaggaccaa cggcatctcg atctcgtact gcggcgacag cgaccccacg
     4381 tcgacgacgt cgatgtcgca ctgggtgaac tcgcggtagc gcccctcctg aggccgctcg
     4441 ccgcgccagg acttctgcat ctgatagcgc cggaacggga acgcgagctt gccggcgttc
     4501 tccaggacgt agcgagcgaa gggaacggtg aggtcgaagt gcaggccgag ggagggctcg
     4561 cgcgagtcgc tggcgtcggc cgccaaccgg ctgatcccgt agatctcctt gtcggcgtcg
     4621 ccgcccttgc cgagcaaccg ctcgaccgac tcgaccgcgc gagtctcgat cgaggcgaac
     4681 ccgtgcagct cgaacacctc acggatgacg tcgaggaagt gctgctcgac gatccgctgt
     4741 tcggggagga actcgaggaa gccgctgatc ggggtgacct tgccggtgct caccgaggga
     4801 tcatcccaga cccggtgtcg ccgtcgcgcg agcgtttcgc cgctgcggcc ccatgcgatg
     4861 gggatgcaag tgctgcgacg gccggggccg atcggttagc gcagcccgcg cagatacgga
     4921 ttggaggccc gctctcgccc gatcgtcgtc aacggtccgt gcccgggaag caccagccgg
     4981 tcgtccggca tcggcaggac gcaatcgcgc agcgaccggt tcatcgccgc cgggtcgccc
     5041 cccgccaggt ccgagcggcc gatcgagccg gcgaagagga cgtcaccgga gatcaccgtg
     5101 gagccgacct cgacgttgat gccctccggg acatccgcca gggtgaacat cacggacccc
     5161 tcggtgtgtc cgggtgcgtg gacgatgccg aaagccatcc cggcgatagt caccgagtct
     5221 ccgtcgcgca attcgcggat gtcgtccggt tcggtccacg tcgcgcgctc cccgaactgc
     5281 gcctccatca tcgcgatcat gccccggtcc agatccgcga gcgggtcccg caggcgatag
     5341 cgatcgtccc catggatgta tgccgcggcg ccgccgccgc ggcataccgg cgtcaccgag
     5401 tagacatggt cgaggtgacc gtgcgtgagc aggaccgccg ccgggcgcag ccgcaactcg
     5461 cgcagcacct cggacagggt gtcggtgacc ccgataccgg ggtcgacgac gacgcactcc
     5521 tccccttcgg cggtcgccag cacgaagcag ttggtgtcga aggccgcagc agggaagccg
     5581 atcatcagca catcgagagc ctatccacgg acaaccttca cgcactcgtg accgggggtg
     5641 gtcagtgtaa gggcctcgcc tgcctagact ggtcgaccgt cggctgtcgt cgcgccttgt
     5701 cggcgcatct aggaggtccg tgctcatgcg cgacaaacaa cccgtgctcg ctgtcatcgg
     5761 cgtgttgctc gccgtgatcg ccttcgtctg ggtggccaac gtggtgacgg cacccaaggt
     5821 cgccgacccg acggccaccg gtgaaccgac cagcacggcg accacgaccg ccaccgacaa
     5881 tccgtcggcg accgccaccg cgacgcccac accgacgccg acccccaagg tgttggccgg
     5941 gtgcacggag gtt
//
LOCUS       DAOMZY010000228         5874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_12794_length_5874_cov_5.994501, whole genome shotgun sequence.
ACCESSION   DAOMZY010000228 DAOMZY010000000
VERSION     DAOMZY010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12794_length_5874_cov_5.994501"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..1069)
                     /gene="era"
                     /locus_tag="PLK64_12185"
     CDS             complement(140..1069)
                     /gene="era"
                     /locus_tag="PLK64_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HPV80551.1"
                     /translation="MTDSGEDAAYRAGFACLVGRPNAGKSTLTNALVGQKVAITSSKP
                     QTTRHTIRGISTTADSQLILIDTPGLHRPRTLLGERLNDLVREALLDVDVVGFCLPAD
                     QRVGPGDQFIAAELAELRRVKRKPVVALATKSDLVSRERLLTHLVAIDALGEWDAIVP
                     CSATRGDQVDEVRTVLVGLLPLSPPLYPSEARTDEPDDVMIAELVREAALEGVRDELP
                     HSLAVVVEEIVPREVPEGHEPMLDVRVSVFVERDSQKAIVIGRGGARLRDVGSTARAA
                     IEELLGSKVYLDLHVKVAKDWQRDPKALSRLGF"
     gene            complement(1062..>1427)
                     /locus_tag="PLK64_12190"
     CDS             complement(1062..>1427)
                     /locus_tag="PLK64_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HPV80552.1"
                     /translation="VGEIADEYDRDEPGVEPIGDGGYRLPAAMHIDDVAELFDLDIDE
                     DEVDSIGGLLTKALGRVPFAGAHAGIAGLSITAERMAHRHRIATVLVRPLVPLGETES
                     EPLVRRLAGDPDRVGGDDD"
     assembly_gap    1428..1527
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1528..1863)
                     /locus_tag="PLK64_12195"
     CDS             complement(<1528..1863)
                     /locus_tag="PLK64_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HPV80553.1"
                     /translation="MSGVVLAQVVVVTVLATVLAFCLSAAEAALQRMTRVRAEELADD
                     GRVGAAALVAVVEQPAPYLAVLAFLRVVAEATTAVLITVAVIALTDSLGTALAISILV
                     MALVSFVLVG"
     gene            complement(1880..2341)
                     /gene="ybeY"
                     /locus_tag="PLK64_12200"
     CDS             complement(1880..2341)
                     /gene="ybeY"
                     /locus_tag="PLK64_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPV80554.1"
                     /translation="MSVDVLNECGYAVDELELVACARHVMDRMKVHPDADLAISLVDE
                     AAMTVLHEKWMDLPGPTDVMSFPMDELRPGRDGVDLQEGVLGDIVLCPAVAEEQARQA
                     GHAPVEELLLLTTHGLLHLLGFDHADPDEKHEMFELQRQLLLTYLAGRGRR"
     gene            complement(2338..3393)
                     /locus_tag="PLK64_12205"
     CDS             complement(2338..3393)
                     /locus_tag="PLK64_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPV80555.1"
                     /translation="MPSASVAAALPTHAITIPPTVSMVTLLGPRDELLDTIERAFPTV
                     DVHVRGNELSLRGPGVDVAVVERLIDELLVVIDAGQPLTRDAVERAIAMLRARTAERP
                     ADVLTLNIISNRGRTIRPKTLGQKRYVDAIDMHTIVFGIGPAGTGKTYLAMAKAVAAL
                     QSKRVNRIILTRPAVEAGERLGFLPGTLSDKIDPYLRPLYDALHDMVDPDTIPRLMAS
                     GTIEVAPLAFMRGRSLNDAFVILDEAQNTSAEQMKMFLTRLGFGSKMVVTGDITQIDL
                     PGGTRSGLAHVRDILADVDDIYFTELTSADVVRHKLVSAIVDAYGASEAKEQAAREAE
                     DHGRHTPRHLAGGANRP"
     gene            complement(3485..3829)
                     /locus_tag="PLK64_12210"
     CDS             complement(3485..3829)
                     /locus_tag="PLK64_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HPV80556.1"
                     /translation="MSAPTCLFCKIVAERIPATVVARSEHSLAFRDIDPQAPTHVLVI
                     PRRHVQNVGELADAPRELADLFELAARVAAQEGLGRGYRMVTNTGSEGGQIVFHAHVH
                     VLGGRPMQWPPG"
     gene            3900..4478
                     /locus_tag="PLK64_12215"
     CDS             3900..4478
                     /locus_tag="PLK64_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HPV80557.1"
                     /translation="MQLERPGARGSARTPGAAVGVTSADEAIAILYAAHWHPLVRLAW
                     FLVHDQGTAEDVVQDAFIATHRNYDRIRDTGAAVAYLRRAVVNGARSVQRHDVVVRRE
                     AIAEAGRADAADRATAASAEAQALRHLGDGAMLAALRRLPDRQREVLVLRYFADWSEA
                     AIADALGIAPGSVKAHAHRGLAALRARLEATE"
     gene            4475..5575
                     /locus_tag="PLK64_12220"
     CDS             4475..5575
                     /locus_tag="PLK64_12220"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HPV80558.1"
                     /translation="MTAHPPGDPHVEARLRDALTTAAERVSPTDRLGAITEAVSTAPP
                     RAGTPRWLLPLAASFLVLAVGLGALTLQQRVGSNAVAASGTHTAVVIAADDPLPAPSR
                     ALPASPGASTPGSTSATRFEWSAPVYYAAPGTPVVRWALYRDFVRATMGDDGLDARVT
                     TAVNLALAQPDVWGQAMPGQDGYLRAWTPGTRVTASVAADRIALTLSAPGVGGLDADA
                     QRMAVQALVWTATAAAQRNVPVAIDVAGAGPVVGGIPAGVFRRPADTTRELAPVWITS
                     PGRFASVWDSRVVIEGQACTFEGTVHWELRLDGAMVRQGFTTATAGCPVRGTWRVDLG
                     VLAPGRYTIRAFESDAATGGPFAEQAVAFTVR"
     gene            complement(5584..>5874)
                     /locus_tag="PLK64_12225"
     CDS             complement(5584..>5874)
                     /locus_tag="PLK64_12225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPV80559.1"
                     /translation="QVRPLARTADVARLLADAALGIVLHEEADRPLASVELPPTGEIV
                     LVVGPEGGIAQTELDAFAAVGVVAVRLGPEVLRTSTAGPAALAVLNGMSRWR"
BASE COUNT          892 a         2091 c         1950 g          841 t
ORIGIN      
        1 gcggtctggg acgcctcatc gtcgggatgg gcgtggacga acaccaaagt cgacataagc
       61 gctcagcatg ccgtgtcagg caggcctcac caaaccagcc gcgcacccgg gcgcgctctc
      121 gcagacccga gtgccgggct cagaacccca accgcgacag ggccttgggg tcgcgctgcc
      181 agtccttggc gaccttgacg tgcaggtcga ggtagacctt cgacccgagc aactcctcga
      241 tcgccgcacg cgccgtcgac ccgacatcgc gcagccgcgc gccaccgcgc ccgatgacga
      301 tggccttctg gctgtcgcgc tcgacaaaga ccgacacccg cacgtcgagc atcggttcgt
      361 ggccctcagg gacctcccgc ggcacgatct cctcgacgac gaccgcgagc gagtgcggca
      421 actcgtcccg cacgccttcc aacgcagcct cacggaccaa ctcggcgatc atcacgtcgt
      481 cgggctcgtc ggtgcgcgcc tccgacggat acagcggcgg cgacagcggt aacagcccga
      541 ccaacaccgt gcgcacctcg tcgacctgat cgccgcgggt cgccgagcag ggcacgatgg
      601 cgtcccattc gcccaaggcg tcgatcgcca cgaggtgggt gagcaggcgc tcccgcgaca
      661 ccaggtcgct cttggtcgcc agcgccacca cgggtttgcg cttgacccgc cgcagctccg
      721 caagctcggc ggcgatgaac tggtcacccg gcccgacccg ctgatcggcc ggcaggcaga
      781 agcccacgac gtcgacgtcc aacagtgcct cgcggaccag gtcgttaagc cgctccccca
      841 acagggtccg cggccggtgc agacccgggg tgtcaatgag gatgagctgg ctgtcggccg
      901 tggtcgagat cccgcggatc gtatgccgcg tcgtctgcgg cttggacgac gtgatcgcca
      961 ccttctgccc gaccaaggcg ttggtcagcg tcgacttgcc cgcattgggt cgccccacga
     1021 ggcaggcaaa accggctcgg tatgccgcgt cctcacctga gtcagtcatc gtcgcctccc
     1081 acacggtcag ggtcaccagc cagccggcgc accagcggct cggactcggt ctcgcccaat
     1141 ggcaccaagg ggcgcacgag caccgtggcg atccggtgcc ggtgagccat cctctcagcc
     1201 gtgatcgaca accccgcgat cccagcatgg gcgccggcga acggcacccg tcccaacgcc
     1261 ttcgtgagca gcccgccgat gctgtcgacc tcgtcttcgt cgatgtccag gtcgaacaac
     1321 tccgcaacgt cgtcgatgtg catcgcggcg ggcaaccggt agcccccgtc cccgatcggt
     1381 tccacccctg gctcgtcccg gtcgtattcg tcggcgatct cccccacnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnacc gacgaggacg aaggacacga gggccatgac
     1561 gaggatcgag atcgccagcg ccgtccccaa cgaatcggtg agggcgatga cggcgacggt
     1621 gatgagcact gccgtggtcg cctcagcgac aacccgcaag aaggccaaga ctgccaggta
     1681 gggggcgggt tgctcgacca cggccacgag ggcagcagcg ccgacccgac cgtcgtcagc
     1741 caactcctcg gcacggaccc gggtcatgcg ctgcaaggcg gcctccgccg ccgacaagca
     1801 gaaggccaac accgtggcca acacggtgac gacgacgact tgggccagca ccacgccgct
     1861 catcgctcac cgactccaat catcgtcggc cgcggccggc gagataggtc aggagcaact
     1921 gccgctgcaa ctcgaacatc tcgtgcttct cgtcggggtc ggcgtgatcg aagcccaaca
     1981 ggtgcaggag accatgggtc gtcagcagga gcagctcctc gaccggagca tgcccggctt
     2041 ggcgggcctg ctcctcggcc accgcgggac acaggacgat gtcgccgagg acgccttctt
     2101 gcaggtcgac gccgtcccgt cccgggcgca actcgtccat ggggaaactc atgacgtcgg
     2161 tcggacccgg caggtccatc catttctcgt gcagcaccgt catcgccgcc tcgtcgacca
     2221 ggctgatggc caggtcggcg tccgggtgca ccttcatccg gtccatgaca tgacgggcgc
     2281 acgccacgag ttccagctcg tcgacggcat acccgcactc gttgaggacg tcgacgctca
     2341 cgggcggttc gctcctccgg ccaggtgccg tggggtatgc cgcccgtggt cctctgcctc
     2401 gcgcgcggcc tgctccttcg cctcggacgc gccgtaggcg tcgacgatgg cgctgaccaa
     2461 cttgtggcgc acgacatcgg cggaggtgag ctcggtgaag tagatgtcgt cgacgtcggc
     2521 gaggatgtcg cgcacgtggg cgagccccga acgggtgccg cccggcaggt cgatctgggt
     2581 gatgtcgccc gtgacgacca tcttcgagcc gaacccgagc cgggtgagga acatcttcat
     2641 ctgctcggcc gaggtgttct gagcctcgtc gaggatgacg aaggcgtcgt tgagcgagcg
     2701 accgcgcatg aaggccagtg gcgcgacctc gatggtgccc gaggccatca gccgcgggat
     2761 cgtgtcgggg tcgaccatgt cgtgcaaggc gtcgtagagc gggcgcagat aggggtcgat
     2821 cttgtcgctc agcgtcccgg gcaggaagcc caaccgctcg cccgcttcga ccgccggccg
     2881 ggtgaggatg atccggttga cccgcttgct ctgcagcgca gcgaccgcct tggccatcgc
     2941 cagataggtc ttcccggtgc cggccgggcc gatcccgaag acgatcgtgt gcatgtcgat
     3001 cgcgtcgaca taccgcttct gccccagggt tttcggccgg atcgtccgac cgcggttgct
     3061 gatgatattg agtgtcagca cgtcggccgg tcgctccgcg gtgcgggccc gcaacatcgc
     3121 gatcgctcgc tcgaccgcat cgcgtgtcag gggttgccca gcgtcgatga cgacgagcaa
     3181 ctcgtcgatc aaccgctcga cgacggccac gtcgaccccc ggcccgcgca acgacaactc
     3241 gttgccgcgg acatggacgt cgaccgtcgg gaaggcccgc tcgatcgtgt ccaacagctc
     3301 gtctcggggt ccgagcagcg tcaccatcga cacggtcgga gggatggtga tggcgtgagt
     3361 gggcagcgcc gcggcgacgg aggccgaggg catgccgcgc gggtgcggct cggtcatggc
     3421 gcagaccagt ctatgaggcg cgcggcatac cgatcgacgt agattcccac cagcttgcgg
     3481 ttcctcaccc cggaggccac tgcatcggcc ggcccccgag cacgtgcacg tgggcgtgga
     3541 agacgatctg gccgccctcg gagccggtgt tcgtcaccat ccggtagccc cggcccaggc
     3601 cttcctgcgc cgcgacccgc gccgccaact cgaacaggtc ggcgagctcc cgcggcgcat
     3661 cggccaactc gccgacgttc tggacgtgcc gccgcgggat gacgagcaca tgggtgggtg
     3721 cctgagggtc gatgtcacgg aaggcgagcg agtgttcgct tcgggcgacg acggtcgccg
     3781 ggatccgttc ggcgacgatc ttgcagaaca ggcaggtggg cgcgctcatg ctgcgagact
     3841 agtcgcgccg gcgaggtcaa ccgacgtccg cggccagccg tccagagagc gtgccgacga
     3901 tgcagctgga acgccccggt gcgcgcgggt ctgcgcgcac acccggcgcc gctgtcggcg
     3961 tcacctccgc cgatgaggcg attgccatcc tgtatgccgc ccactggcac cctctcgtgc
     4021 ggctggcctg gttcctcgtc cacgaccaag gcaccgcgga ggacgtcgtc caggacgcgt
     4081 tcatcgccac ccaccgcaac tacgaccgga tccgggacac cggcgcggcg gtcgcctatc
     4141 tgcgccgggc cgtcgtcaac ggcgcccgct ccgtgcagcg gcacgacgtt gtcgtccggc
     4201 gggaagcgat cgccgaggcc ggccgagcgg acgctgccga ccgggccacg gcggccagtg
     4261 cggaggcgca ggccctgcgc catttggggg acggcgccat gttggccgcc ctacgacgcc
     4321 tgcccgaccg ccaacgagag gtcctcgtgc tgcgctactt cgccgactgg tccgaggccg
     4381 ccattgccga cgccttgggc atcgcccccg gctcggtcaa agcgcacgcc caccgcggcc
     4441 tggctgccct gcgggcccga ttggaggcga ccgaatgacg gcccacccac caggagaccc
     4501 gcacgtggaa gcgcggctgc gcgatgccct caccacggcc gccgagcgag tctccccgac
     4561 tgatcggctc ggtgcgataa ccgaagcggt gtcgacggca cccccgcgag ccggcacacc
     4621 ccggtggctg ctcccgctgg cggcgtcgtt cctcgttctc gcagtggggc tgggcgcatt
     4681 gaccctgcag cagcgggtcg gcagcaacgc ggtggccgca tccgggaccc acacggctgt
     4741 ggtgatcgcg gccgacgacc cgctgccagc accctcacgg gcgctgcccg cgtcgcccgg
     4801 tgcatccacc ccgggctcga cgagcgcgac ccgtttcgag tggtccgccc ccgtctatta
     4861 cgcagcaccg gggacgcccg tcgtgcggtg ggcgctctat cgcgacttcg tgcgcgccac
     4921 gatgggcgat gacggcctcg atgcccgagt caccacggcc gtcaacctcg ctcttgcaca
     4981 gccagatgtc tggggccagg ccatgccggg ccaggacggc tacctgcgcg cctggacgcc
     5041 cggcacgcgc gtcacggcca gcgtcgcggc ggatcggatc gccctcaccc tcagcgcacc
     5101 tggtgtcggc gggctcgacg cggacgcgca acgaatggcc gtgcaggcgt tggtgtggac
     5161 cgccaccgcg gcggcccaac gcaacgtccc ggtagccatc gacgtggccg gcgccggccc
     5221 ggtggtgggc ggcatacctg ctggggtgtt ccggcgaccg gctgacacca cccgggagtt
     5281 ggcccccgtc tggatcacgt caccggggcg gttcgcctcg gtgtgggact cgcgcgtggt
     5341 catcgagggc caggcgtgca cgttcgaggg caccgtccat tgggagttgc ggctcgacgg
     5401 agcgatggtc cgccagggat tcacgaccgc gacggccggg tgccccgtcc gagggacctg
     5461 gcgggtcgac ctcggggtgt tggcgccggg ccgctacacg attcgagcat tcgagtcgga
     5521 tgccgcgacc ggtggcccct tcgccgagca ggcggtggcc ttcacggtcc gttgaccctc
     5581 ggctcagcgc cagcgcgaca tcccgttgag gaccgccagc gccgccgggc ccgccgtcga
     5641 ggtccgcaac acctcgggcc cgagccggac cgcgaccacc cccaccgccg cgaaggcatc
     5701 caactcggtc tgcgcgatgc cgccctcggg gccgacgacg aggacgatct cccccgtcgg
     5761 cggcaactcg acgctcgcca gcggacggtc ggcctcctcg tgcaggacga tgcccagagc
     5821 ggcatcggcc aacagccgcg ccacatcggc ggtgcgggcc agcggcctga cctg
//
LOCUS       DAOMZY010000229         5851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_12866_length_5851_cov_4.994134, whole genome shotgun sequence.
ACCESSION   DAOMZY010000229 DAOMZY010000000
VERSION     DAOMZY010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12866_length_5851_cov_4.994134"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PLK64_12230"
     CDS             <1..487
                     /locus_tag="PLK64_12230"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HPV80560.1"
                     /translation="RFQAGAGLAVLAGRFTPALRAVAGRHLLKLAVATEKTAFLVVRH
                     GDEAVTIEVVEPPAAAAHVSYRPGLRHPITTGAPGIALLAGEPPHADERPAVTQARSL
                     GWATSTSEVIEGFRAVAAPVIDTSGVCRAAIAVVFAGSGNVSGVADRVMAHALALGLD
                     L"
     gene            complement(512..748)
                     /locus_tag="PLK64_12235"
     CDS             complement(512..748)
                     /locus_tag="PLK64_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80561.1"
                     /translation="MAALVLTMVVCLLLGLAVVLAVAVPARREGKDLLTPRGEEVVAR
                     VRERTEHVASATKERTEEVLATAKDKVGEVLPSR"
     gene            complement(876..4472)
                     /gene="smc"
                     /locus_tag="PLK64_12240"
     CDS             complement(876..4472)
                     /gene="smc"
                     /locus_tag="PLK64_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HPV80562.1"
                     /translation="MYVKSVTLKGFKSFASATTLRLEPGITCIVGPNGSGKSNVVDAL
                     AWVMGEQGAKSLRGGKMEDVIFAGTAGRAPLGRAEVALTIDNTDGALPIDYSEVTISR
                     TMFRNGGSDYAINGTSCRLLDIQELLSDSGIGREMHVIVGQGQLDAVLRATPEERRGF
                     IEEAAGVLKHRKRKERALRKLETMEANLTRVQDLTTEIRRQLGPLGRQAETARKAGVI
                     QADVRDARLRLIADDLVQLTATLEQEVADETALIARRTEVETALASLGARLTALEVEA
                     DETRPQLAAAQQRWFALTRLVERFTSTATLAGERVRLLAEDEADNAGPTSGPAIPRDP
                     DALRAQAAALRTEHEELLAEIAAATAALTAAVTARTESERRYAAEQQRLAALVRAAAD
                     RREGLAKLAGQVAAKASRVEAGEAEVGRLQAGVGQILARSLEAEREFARLEASIAVDE
                     EGEEGLDAAYEKANAELTALQAEQERLREQERTADRDRQSATARAEALALSLTRRDGA
                     ATLLGSDGNPREGVLGAVSSLIEVVDGHEAAIAAALGWAADAVAVASIDAAVAGVAAL
                     RAAESGRAGMLVGATARPGASGSRAALPVGARWAADVVSAPDAIAPAVHQLLDGVALV
                     ADVAAARTLVTEGITAVTPDGDVYAPGFVVGGDRGAPSLLEMESALEAARAAAAAAGR
                     LVEQAKFALAGVRDRIADVQERVEASLERLHESDARMSAVGEQLGHLGSVVRGAKGEA
                     ERTERAIAAAVAALDADRAAHAELAQRLAAASEEVPVDAEPDPSVRDALEAEASSART
                     QETEARLTLRTREERARAVAERAETLEAAAVAELAARERQAARRERRARESRVATAVR
                     SGAAYAAARLRESAERAGEAARAGEAARAERDAERSRLRREMGGFQDELRELTDSVHR
                     DEVARAQQRLRIETLTGRAMEEYGVDAEVLVEEFGPHQLVPVIPDPNDPDTDPDALPE
                     PTPFIREAQEKRLRSAERKLAALGRVNPLALEEYAALEERHAFLTTQLEDLKASKRDL
                     LDIVREIDERVEQAFVSAFHDTAAQFEGVFSRLFPGGEGRLLLTDPDNMLTTGLEVEA
                     RPPGKKIKRLSLLSGGERSLVAVALLVAIFKARPSPFYIMDEVEAALDDANLGRLLTL
                     FEELRDSSQLIVITHQKKTMEIADALYGVTMRGDGVTTVVSQRMRDVALATP"
     gene            complement(4536..5570)
                     /locus_tag="PLK64_12245"
     CDS             complement(4536..5570)
                     /locus_tag="PLK64_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80563.1"
                     /translation="MRRPDVPGIRPHLPLRDAEGADRFQAFLVSAVASIAVTRAFLVL
                     TGYPQLGGGSIDGGLHLAHLLWGGLGMVIAILVTMLFISRTARTVAAVIGGVGFGLFI
                     DEVGKFVTGDNDYFFEPVAAIIYATFVVIFLIVRLVVSRTPLTPREQLINAVELLKDS
                     AAHDLDDVERARLVALLRAADPADLLVAPLLALVDGLPPQRPSRSWIAQGYAAARRLV
                     MSAPRLEVVKRTSVWAFTAFCGLALADALWAAGHDPSLQNRVYVAVAAGSALVAVVAL
                     VGWLRGPRGRALQLFEVALLAQVLGVQFFRLLAASFQGYLTAFVSLALIGLCRAMLFE
                     HRTRHAHDGG"
     gene            complement(5563..5823)
                     /locus_tag="PLK64_12250"
     CDS             complement(5563..5823)
                     /locus_tag="PLK64_12250"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HPV80564.1"
                     /translation="MHLDAALVHLLAERFRCTQQVGVLKATVGLPPSDPSREEQQMSR
                     LRELAAEAGLDPVFAEKFLGFIIAEVIRNHGTIAQRATIPDA"
BASE COUNT          856 a         2094 c         2079 g          822 t
ORIGIN      
        1 ccgcttccaa gccggtgccg ggctggcggt cctcgccggg cgcttcaccc cggccctgcg
       61 cgccgtcgcc ggtcggcacc tgctcaaact tgccgtggcc accgagaaga cggcgttcct
      121 cgtcgtgcga catggcgacg aggcggtcac catcgaggtc gtcgagcccc cggcggccgc
      181 ggcccacgtg agctaccgac ccggcctgcg tcacccgatc accaccggcg cgcccggaat
      241 cgccctcttg gcgggcgaac ccccgcacgc cgacgagcgg cccgcagtca cgcaggcacg
      301 gagtctgggc tgggcaacaa gcaccagcga ggtgatcgaa ggcttccgcg cggtcgcggc
      361 accggtcatc gacacctcag gggtatgccg cgcggccatc gccgttgtct tcgccggaag
      421 cggcaacgtg tcgggtgtgg ccgatcgtgt gatggcacac gccctagcgc tcggtctgga
      481 cctctgaagg cccagcgcga tcgctgacag gtcagcggct gggcaggacc tcgccgacct
      541 tgtccttggc cgtggccagg acctcttccg tgcgttcctt ggtcgccgag gcgacgtgct
      601 cagtgcgctc gcgaacgcgc gcaacgacct cctcaccccg gggggtgagc aggtcctttc
      661 cctcccgacg ggccggaaca gcgacagcga gcaccacagc caacccgagc aggaggcaca
      721 caaccatcgt gaggacgagt gcagccatgg gaccaagtgt cccaaatcgc cagccgaagc
      781 ggggcctcgg cgcgcctatg acgtgacatg gatcacggca taccggagcg ggtcgggcgc
      841 cctcgatctc acgattcggg acgcggccgt caagatcagg gggtcgcgag cgcgacgtcg
      901 cgcatccgct gactcactac ggtggtcacg ccgtccccgc gcatggtcac gccatagagg
      961 gcgtcggcga tctccatcgt cttcttctgg tgggtaatca caatgagttg gctggaatcg
     1021 cgcaactcct cgaagagcgt cagcaatcgg ccgaggttgg catcgtcgag ggccgcctcg
     1081 acctcgtcca tgatgtagaa cgggctcggc cgggccttga agatcgccac gagcagggcc
     1141 accgccacca gtgaccgctc cccaccggac agcaacgaca accgcttgat cttcttgccg
     1201 ggcgggcgcg cttcgacctc gagaccggtc gtgagcatgt tgtcggggtc ggtgagcagc
     1261 agccgcccct cgcctccggg gaacaaccgc gagaagaccc cctcgaactg cgcggccgtg
     1321 tcgtggaacg ccgacacgaa ggcctgctcg acccgctcgt cgatctcccg gacgatgtcc
     1381 aacaggtcgc gtttggaggc tttgaggtct tcgagttggg tggtgaggaa cgcgtgccgc
     1441 tcttcgagcg cggcatactc ctccaacgcg agggggttga cccgcccgag agcggcgagc
     1501 ttgcgctcgg ccgaccgcag tcgcttctcc tgggcctccc ggatgaaggg ggtcggctcg
     1561 ggcagcgcgt cggggtcggt gtcggggtcg ttggggtcgg ggatgaccgg gacgagttgg
     1621 tgcgggccga actcctcgac gagcacctcg gcgtcgacgc cgtactcctc catcgcgcgc
     1681 cccgtgagcg tctcgatccg cagccgttgc tgggcgcggg cgacctcgtc gcggtgcacc
     1741 gagtcggtga gctcgcgcaa ttcgtcctgg aacccaccca tctcacgccg cagccgggag
     1801 cgttcggcgt cgcgctcggc gcgggccgcc tcccccgcgc gggccgcctc ccccgcgcgt
     1861 tcggcgctct cccgcaaccg ggccgcagcg tatgccgccc ccgaccggac cgcggtcgcc
     1921 acccgggact ccctggcccg acgctcccga cgagcggcct ggcgctcacg cgcggcgagc
     1981 tcggcgacgg ctgcggcttc gagggtctcg gcgcgttcgg caacggcgcg ggcgcgctct
     2041 tcgcgggtgc gcagggtcaa ccgggcttcg gtctcctggg tgcgggcact gctggcctcg
     2101 gcctccaggg cgtcccgcac gctcgggtcg ggctcggcat cgacagggac ctcctccgac
     2161 gccgcagcca gtcgctgcgc caactcggcg tgcgcggctc ggtcggcgtc gagagcagcg
     2221 accgcggcgg cgatggcgcg ttcggtgcgc tcggcctcgc ccttggcgcc gcggacgacg
     2281 gagccgaggt gacccaactg ctcgccgacg gcgctcatcc gggcatcgga ctcgtgcagc
     2341 cgctccaggc tggcctcgac ccgctcctgc acgtcggcga tgcgatcccg cacgcccgcc
     2401 agcgcgaact tggcctgctc gaccaggcgt cctgcggcgg cagcagcggc acgagccgcc
     2461 tccaacgcgg actccatctc caacaggctc ggcgcgccgc gatcaccacc gacgacgaag
     2521 ccgggcgcgt agacgtcgcc gtcgggagtg accgcagtga tcccctcggt gacgagcgtt
     2581 cgggcggctg cgacatcggc gaccagggcc accccgtcga gcaactggtg cacggccggc
     2641 gcaatggcat ccggcgcgct gacgacatcc gcggcccacc gcgccccgac cggcagcgcg
     2701 gcccgactcc cggacgcacc gggtcgcgca gtcgcaccga cgagcatccc ggcccgcccg
     2761 ctctcagctg cgcgcaaagc cgccacgccc gccactgcag catcgatcga tgccaccgcg
     2821 accgcatcgg ccgcccaccc cagggcggcg gcgatcgctg cctcatgacc gtcgacgacc
     2881 tcgatgagcg acgacaccgc accgaggacg ccctcgcgcg ggttgccgtc tgagccgaga
     2941 agggtggccg cgccgtcccg tcgggtgagg gacagggcca gcgcctcggc gcgcgccgtc
     3001 gcggactgcc ggtcacggtc ggcggtgcgt tcctgctcgc gcaaccgctc ctgctcggcc
     3061 tgcaacgccg tcagctcggc attggccttc tcgtatgccg cgtcgagacc ctcctcaccc
     3121 tcctcgtcga cggcgatcga cgcctcgagc cgggcgaact cgcgctcggc ctcgagcgat
     3181 cgggcaagga tctggccgac gccggcctgc aggcgcccaa cctcggcctc gcccgcttcg
     3241 acccgggacg ccttcgccgc gacctgaccg gcgagtttgg ccaggccttc gcggcggtcg
     3301 gcggccgcgc ggacgagggc cgcgaggcgt tgctgctcag cggcataccg gcgttcggac
     3361 tcggtgcgcg cggtgacggc ggccgtcagg gctgctgtcg cggcggcgat ctcggcgagg
     3421 agttcctcgt gctcagtgcg cagggcggct gcctgggcgc gcagcgcgtc ggggtcgcga
     3481 gggatggcag gcccggacgt agggccggcg ttgtcggctt cgtcctcggc aagcaggcgc
     3541 acccgctccc ctgccagcgt ggcagtcgac gtgaaccgtt cgacgagccg ggtgagggcg
     3601 aaccagcgtt gctgagcggc ggccagttgg gggcgggtct cgtcggcctc gacctcgagc
     3661 gcggtgaggc gggctccgag cgaggccagc gcggtctcga cctcggtgcg ccgggcgatg
     3721 agtgccgtct cgtcggcgac ctcctgctca agagtcgccg tgagttggac caggtcgtcg
     3781 gcgatgagcc gaagccgcgc gtcccgcacg tcggcctgga tgacgccggc cttgcgagcg
     3841 gtctcggcct ggcggcccag cgggccgagc tgacggcgga tctcggtcgt gaggtcctgg
     3901 acccgggtga ggttggcctc catcgtctcg agtttgcgca gcgcgcgttc cttgcgcttg
     3961 cggtgcttga ggaccccggc ggcctcctcg atgaagccgc ggcgctcctc gggggtcgct
     4021 cgcaggaccg cgtcgagttg accctggccg acgatgacgt gcatctcccg gccgatgccg
     4081 ctgtcggaga gcaactcctg gatgtccagc agccggcagg acgtgccgtt gatggcgtag
     4141 tcggacccgc cgttgcggaa catcgtccgg ctgatcgtca cctcggagta gtcgatcggc
     4201 agcgcgccgt cggtgttgtc gatggtgagg gccacctcgg cccggcccaa cggggcacgc
     4261 ccggcggtgc cggcgaagat gacgtcctcc atcttgccgc cgcgcaggct cttggcgccc
     4321 tgctccccca tgacccaggc gagggcgtcg acgacgttgg acttgcccga cccgtttggg
     4381 ccgacgatgc aggtgatgcc tggttcgagg cgcaacgtcg tcgcggaggc gaacgacttg
     4441 aaccccttga gggtcacgct cttgacgtac aaggtgacgg tggcttcctg ctactggtct
     4501 ggcgaggtcg gatctggcga atggtcccgc ccaggctacc cgccgtcgtg ggcatgccgg
     4561 gtccggtgct cgaagagcat cgctcggcac agcccgatga gggccaacga cacgaaggcc
     4621 gtgaggtagc cctggaagga ggcagcgagc agccggaaga actgcacacc gaggacctgc
     4681 gccaagaggg cgacctcgaa caactgcagg gcgcggcccc ggggcccgcg gagccacccg
     4741 acgagggcca ccacagccac gagcgctgag ccggcggcca cggcgacgta gacgcggttc
     4801 tgcagcgacg ggtcgtgacc ggccgcccac agcgcatcgg ccagcgcgag gccgcaaaag
     4861 gccgtgaagg cccacaccga ggtccgcttg accacttcca ggcgtggcgc ggacatgacc
     4921 agacgccgcg cggcggcata cccctgggcg atccaggacc gcgacgggcg ctgagggggc
     4981 aggccgtcaa ccagcgccag cagtggcgcg accagcaggt cggcgggatc cgcggcgcgc
     5041 agcagggcga ccaaccgggc ccgctcgacg tcgtcgaggt cgtgcgccgc cgagtccttg
     5101 agcagttcca cggcgttgat gagttgttcg cggggcgtga ggggggtccg gctgacgacg
     5161 aggcgcacga tgaggaagat gacgacgaac gtcgcataga tgatcgccgc gaccggctcg
     5221 aagaagtagt cgttgtcccc cgtgacgaac ttgccgacct catcgatgaa caacccgaac
     5281 ccgaccccac cgatgacggc agccaccgtg cgcgctgtgc ggctgatgaa cagcatcgtc
     5341 acgaggatgg cgatcaccat gccgaggcca ccccacaaca ggtgagcgag gtgcagaccg
     5401 ccgtcgatcg acccgccgcc caactgcggg tagcccgtga ggacgaggaa ggcgcgggtc
     5461 acggcgatcg acgcgaccgc gctgacgagg aacgcctgga accggtcggc tccctcggcg
     5521 tcccgcagcg gcaggtgcgg ccggattccc ggcacgtcgg gcctacgcat caggaatggt
     5581 ggcccgctga gcgatcgtgc cgtggttgcg aatgacctcg gcgatgatga aacccaggaa
     5641 cttctcggcg aagaccgggt ccaggcccgc ctcagccgcg agctcgcgca gccgcgacat
     5701 ctgctgctcc tcgcgagacg ggtcggacgg cggcagcccc acggtcgcct tgaggacccc
     5761 cacctgctgc gtgcagcgga agcgctcagc cagcaggtgg accagggccg cgtcgaggtg
     5821 caggcgccac cggcgcgcgg cggccggccc g
//
LOCUS       DAOMZY010000230         5841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_12900_length_5841_cov_6.872624, whole genome shotgun sequence.
ACCESSION   DAOMZY010000230 DAOMZY010000000
VERSION     DAOMZY010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12900_length_5841_cov_6.872624"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PLK64_12255"
     CDS             complement(<1..440)
                     /locus_tag="PLK64_12255"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPV80565.1"
                     /translation="MQISPSILSADFANLEAELESVSNADWAHVDVMDNHFVPNLTLG
                     LPVVESLLRVSPLPIDCHLMISDPDRWAPAYAEAGAGSVTFHIEAAADPRGLARTLRS
                     LGSRAGMALKPATPWAPYEELLPDLDMVLVMTVEPGFGGQSFMA"
     gene            complement(450..1934)
                     /locus_tag="PLK64_12260"
     CDS             complement(450..1934)
                     /locus_tag="PLK64_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPV80566.1"
                     /translation="MSDERRRRGGPAQKSRQRPAERARVGDPARRAAYTVLRAVADGA
                     YANLELPTVLRAQRVSGRDAAFATELTYGTLRLQGRYDPIIALAAGRALTAIDANVLD
                     TLRLGCHQVLGMRVPAHAAVAETVALARQVNGAGAAGFVNAVLRRVSERTPQEWLDAI
                     TPEGSDAVTRLAITQSHPTWIVKALREALLGHGRSTRETVDADLGSLLAADNEAAAMS
                     LVARPGLCTVEELQRAGATPGRWARTAATLPGGDPGALAVVRDGRAAVQDEGSQLVAL
                     ALAAVPVESTGPQRWLDLCAGPGGKAGLLAALALEQGASLVANEVSEHRAELVRHTLR
                     PALAAGSPGQVQVRVGDGREIGELEPAAYDKVLVDAPCTGLGALRRRPEARWRRTPDD
                     LAVLTKLQRELLASALDATRPGGVVAYVTCSPHLSETVFAVRDVVKKRSDVEILDAGE
                     SLRDKDGHALAGSGSGWFAQLWPDVHGTDAMFLALLRKVAPSRS"
     gene            complement(1927..2865)
                     /gene="fmt"
                     /locus_tag="PLK64_12265"
     CDS             complement(1927..2865)
                     /gene="fmt"
                     /locus_tag="PLK64_12265"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPV80567.1"
                     /translation="MRLVFAGTPEVALPSLEALIGSSHEVVGVVTRPDAPAGRGRSLL
                     PSPVRVRAQEHGIPVLTPRTPRDPDFQQELAALAPDCCPVVAYGALVPQAALDIPRHG
                     WVNLHFSLLPAWRGAAPVQHAIRSGDEVTGATTFLLEQGLDTGPIFGVMTETIRPTDT
                     SGDLLGRLALAGAGLLVATLDGIEAGHLVAKPQPGEGISHAPKVEVEDARVDWGLPAL
                     AVDRLIRSCTPAPGAWTTFRGERLKLGPLGPAGEAAPTLAPGDLLVTKAAVYAGTATS
                     PVRLGLVRAHGKKEMPAADWARGVRIESGERLGSDV"
     gene            complement(2871..3413)
                     /gene="def"
                     /locus_tag="PLK64_12270"
     CDS             complement(2871..3413)
                     /gene="def"
                     /locus_tag="PLK64_12270"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPV80568.1"
                     /translation="MSVKEIRLFGDPILRTPAAAVVDFDRELSQLVKDLTDTMMDAPG
                     AGLAAPQIGVGLRVFTYFVDGVVGHLVNPSLELSVEMQDGEEGCLSFPGMKYPTPRSL
                     RVVATGQDEHGEPVLLEGSELLARAVQHETDHLDGILFIDRMDPAQRKLAMKAIRESD
                     WVGGPPIVRISPHNTFGLGV"
     gene            complement(3493..4740)
                     /locus_tag="PLK64_12275"
     CDS             complement(3493..4740)
                     /locus_tag="PLK64_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV80569.1"
                     /translation="MKLTEAVGALRERRFAWYFAARTTTSIGSAMTSVALAFAVLHIS
                     DDPGALAQVLAAHMAAMVIFLLLGGVVADRYSRTVIMQTSHALTFLTQGMAAYLVLTD
                     TAQIWHLVALESVNGAVSAFGMPAMMGVVPLVVGPDRIQQANALLSFSRSSITIVGPA
                     LAGALVVGAGPGWALLVDSALFLVAIPMLRQVALPGRDRNVTASPSVIAELREGWGEF
                     TSRTWVWLIVVAFGVLNAVHMGAIAVLGPVIAKASPVGEQGWGLALSAEAAGTVVMTL
                     ALMRTRLRRPLVAGMLGVSLIALPMLVLGLSPRTDLLIAAFFVAGAGVEVFGVGWNTS
                     LHQHIPEAVLSRVSSYDALGSFVAMPIGAMAFGWLATAYDHRSILLVSAIGYAVLSLA
                     TLAAPSVRGLRQVVADAGSETAD"
     gene            complement(4737..>5841)
                     /locus_tag="PLK64_12280"
     CDS             complement(4737..>5841)
                     /locus_tag="PLK64_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483404.1"
                     /note="binding of PriA to forked DNA starts the assembly
                     of the primosome, also possesses 3'-5' helicase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="primosome assembly protein PriA"
                     /protein_id="HPV80570.1"
                     /translation="TPATLRAAAARVVVAGEGEVGRERDSAAASAQLPPSAWRIVHEG
                     LTRGPVLVQVPRGGYRPSLSCQVCRAPVRCTACHGPMGQDDPRHPPACTWCGTGFGVA
                     GFRCPQCDSTAWRAAILGSRRTAEELGRAFPGVPVITSASGEVAGQVAATPALVIATP
                     GAEPRATQGYAACLLLDAWATLSRPELDAPVEALRRWLAAAALCRSALDGGVVVLAGV
                     PDGPPFPAVEALVRWDPVWLAERELTERAELGLPPAVVMAELVGTRHAVAAAVSDIVS
                     GADLDVTTFGPLPVAVSVSASASVAAPVAAPVAASASSAADAPHVRMVLRVPHAQAGT
                     LARALAATKAIRSARKDPEPLHVRMGWTGEGSR"
BASE COUNT          937 a         2121 c         2048 g          735 t
ORIGIN      
        1 tcggccatga acgactggcc gccgaagccc ggctcgacgg tcatgacgag caccatgtcc
       61 agatcgggca gcaactcttc gtagggcgcc cacggggtcg ccggcttgag cgccatcccg
      121 gcccgggacc ccaacgaccg cagggtgcga gccagcccgc gcgggtcggc cgccgcctcg
      181 atgtggaagg tcaccgatcc ggcacccgct tcggcatacg ccggagccca gcgatcggga
      241 tcggagatca tcaggtgaca gtcgatcggc agcgggctca cccgcaggag cgactcgacg
      301 accggcagcc ccagcgtgag gttcggcacg aagtggttgt ccatgacgtc cacgtgcgcc
      361 cagtcggcgt tgctcaccga ctcgagctcg gcctccaggt tggcgaagtc cgcggacagg
      421 atgctcggtg agatctgcac gacccaaccc taggaccggc tcggcgccac cttgcgcagc
      481 agcgcgagga acatcgcgtc ggtgccgtgc acgtccggcc agagttgggc gaaccacccg
      541 ctcccggacc ccgccagcgc atgtccgtcc ttgtcccgca acgattcccc agcgtcgagg
      601 atctcgacgt ccgaacgctt cttgaccacg tcgcgcaccg cgaacaccgt ctccgacaga
      661 tgcgggctgc aggtgacgta ggcgaccacc ccgccggggc gggttgcgtc cagggcactg
      721 gccagcaact cgcgctgcag tttggtgagc accgcgaggt cgtcgggggt tcgccgccac
      781 cgcgcttcgg ggcgacgacg cagggcgccc aggcccgtgc acggcgcatc cacgaggacc
      841 ttgtcgtatg ccgcgggctc caactcgccg atctctcggc cgtcccccac ccgcacctgc
      901 acctgccctg ggctccccgc cgcaagcgcc ggccggaggg tatgccgcac caactcggcc
      961 cgatgttcgc tcacctcgtt ggcgaccagc gaagcaccct gttcgagggc gagcgcggcc
     1021 agcagacccg ccttgccacc cgggccggcg cacaggtcga gccagcgctg cgggccggtg
     1081 ctctcgacgg ggaccgccgc taacgcgagc gcgaccaact gggagccttc gtcctgcact
     1141 gcggcgcgcc cgtcacgcac caccgcgaga gcccccgggt cgccacccgg cagggttgcc
     1201 gccgtgcgcg cccagcgccc cggggtcgct ccggcgcgtt ggagctcctc gaccgtgcac
     1261 agacccggcc gggcgacgag ggacatggct gccgcctcgt tgtcggccgc cagcagactg
     1321 cccaggtctg cgtcgacggt ctcccgtgtc gaacggccat ggccgagcag cgcctcgcgc
     1381 agcgccttga cgatccacgt cgggtgcgac tgggtgatcg cgagacgcgt caccgcgtcc
     1441 gaaccctcgg gcgtgatggc gtccagccac tcctgcgggg tccgctcgct cacccggcgc
     1501 aggaccgcgt tgacgaaacc ggccgcgccg gcgccgttga cctgcctggc taaggccacg
     1561 gtctcggcga cggcggcgtg ggccgggact cgcatcccca acacctgatg acagcccagt
     1621 cgcagggtgt ccaggacgtt ggcgtcaatc gccgtgagtg cccgcccggc cgcgagcgcg
     1681 atgatcgggt cgtagcggcc ctgcagccgc agggtcccgt aggtgagctc cgtggcgaac
     1741 gcggcgtcgc gcccagacac ccgctgggcg cgcagcaccg tggggagttc gaggttggca
     1801 taggcaccgt cagcgacggc tcgcaggacg gtgtatgccg cgcgccgcgc cggatcgccg
     1861 acccgagctc gttcagccgg ccgttgccgg gacttctgcg ccgggccgcc ccgtcgacgt
     1921 cgctcgtcag acatccgagc ccagccgctc ccccgactcg atccgcacgc cgcgcgccca
     1981 atcggccgcc ggcatctcct tcttgccgtg cgcgcggacc aggccgaggc ggaccgggga
     2041 ggtggcggta ccggcataca ccgctgcctt ggtgaccaac aggtcgccgg gagccaacgt
     2101 cggggctgcc tcgccagcgg gcccgagcgg accgagtttg aggcgttcgc cgcggaacgt
     2161 cgtccacgcc ccaggcgccg gggtgcacga gcggatcagt cggtcaacgg ccagcgcggg
     2221 gagcccccaa tcgacccggg catcctcgac ctccaccttc ggcgcatgcg agatcccttc
     2281 gcccggttgg ggtttggcca ccaggtggcc ggcctcgatg ccgtcgagag ttgccacgag
     2341 cagcccggct ccagcgagtg ccagccgccc gagcaggtcg ccgctggtgt cggtgggtcg
     2401 gatcgtctcg gtcatcacgc caaagatcgg gcccgtgtcc aacccctgct cgaggaggaa
     2461 ggtcgtggcg ccggtgacct cgtcgcccga gcggatcgcg tgctggaccg gtgcggcgcc
     2521 ccgccaggcc ggcagcagcg agaagtgcag gttgacccac ccgtgccgcg gaatgtccag
     2581 ggcagcctgc gggaccagcg cgccgtaggc gaccaccggg cagcagtccg gtgcgagcgc
     2641 cgccaactcc tgctggaagt ctgggtcgcg gggtgtgcgc ggagtgagca ccggtatgcc
     2701 gtgctcctgc gcccgcacgc gcacgggcga gggcagcagg ctgcggccgc gaccggccgg
     2761 ggcgtcgggg cgggtgacga caccgacgac ctcgtgggat gacccgatga gagcctccag
     2821 gctcggcagc gccacctcgg gtgttccagc aaagaccagg cgcatcgcgc tcacaccccc
     2881 agaccgaagg tgttgtgtgg gctgatccgc acgatcggtg gcccgccgac ccagtcggac
     2941 tcgcggatgg ccttcatggc gagcttgcgt tgggccgggt ccatccggtc gatgaagagg
     3001 atgccgtcga ggtggtcggt ctcgtgctgc acggcccggg cgagcagttc gctgccttcc
     3061 aggagcaccg gctcaccgtg ctcgtcctga ccggtcgcga cgacgcgcaa cgaccggggg
     3121 gtcgggtatt tcatgccagg gaaggagagg cagccctcct cgccgtcctg catctccacc
     3181 gacaactcca gcgacgggtt gaccaggtgc ccgacgacgc catcgacgaa gtaggtgaac
     3241 acccgcagcc cgacgccgat ctgcggcgcc gccaatccag caccgggcgc gtccatcatc
     3301 gtgtcagtga ggtccttcac cagttggctc agctcccggt caaagtcgac gaccgccgcg
     3361 gcaggggtcc gcaggatcgg gtcgccgaaa aggcggatct ccttgaccga caacgctgtt
     3421 ccttcgagag ccaggaggcc gggccaggca ccccgatcag ggctcgacac tcggcatcac
     3481 ggaggtgcca gtctagtcgg ccgtttccga tccggcgtcg gcgacgacct ggcgcagccc
     3541 ccgcaccgac ggtgcggcca gcgtggccag gctgaggacg gcatacccga tggcgctgac
     3601 gagcaggatc gagcggtggt cgtaggcggt ggcgagccac ccgaaggcca tcgccccgat
     3661 cggcatcgcc acgaaagacc cgagcgcgtc ataactggac acccgggaca acaccgcctc
     3721 cgggatgtgt tggtgcagcg aggtattcca cccgacgccg aacacctcga ccccggcacc
     3781 ggccacgaag aacgccgcaa tgagcaggtc ggtgcgaggg gacagcccga gcacgagcat
     3841 cgggagcgcg atgagcgaga ccccgagcat ccccgcgacg agcgggcgcc gaaggcgagt
     3901 ccgcatgagg gcgagggtca tgacgacggt cccggcggcc tctgccgaca acgcgaggcc
     3961 ccagccctgc tcgcccaccg gcgaggcttt cgcgatgacc gggccgagca cggcgatcgc
     4021 ccccatgtgc acggcgttga gcactccgaa tgcgacgacg atgagccaca cccaggtgcg
     4081 ggaggtgaac tccccccatc cctcgcgcaa ctcggcgatg accgagggag acgccgtgac
     4141 gttgcggtcg cggccgggca gggccacctg ccgcagcatg gggatggcga cgaggaacaa
     4201 cgccgagtcg accagcagcg cccaaccggg cccggcgccg accacgaggg ctccggcgag
     4261 cgccggaccg acgatggtga tggaacttcg gctgaaggac agcagtgcgt tggcctgctg
     4321 gatgcgatcg ggccccacga cgagggggac gacccccatc atcgctggca tgccgaaggc
     4381 cgacaccgcg ccgttgaccg actcgagggc gaccaggtgc cagatctgcg cggtgtcggt
     4441 gaggacgagg tatgccgcca tcccctgagt gaggaaggtc aaggcatgcg aggtctgcat
     4501 gatgacggtc cgcgagtagc gatcggcgac gaccccgccg agcaacagga agatgaccat
     4561 cgccgccatg tgggcggcca ggacctgggc gagggctccc gggtcatcgg agatgtgcag
     4621 gacagcaaag gccagcgcga cgctggtcat cgccgacccg atgctggtcg tcgtcctggc
     4681 ggcgaagtac cacgcgaacc gccgctcacg cagggcgcca acagcctcgg tcagcttcat
     4741 cgggacccct cccccgtcca gcccatccga acgtgcagcg gctcggggtc cttgcgggcg
     4801 ctgcgaatcg ccttggtggc cgccagtgcc cgggccagcg tgccagcctg ggcatgtggc
     4861 acccgaagga ccatgcgcac gtgcggcgcg tccgctgcac tcgacgcgga cgcagccaca
     4921 ggcgcggcca caggcgcagc cacagacgcg gacgcagaca cagacacagc gaccggcaac
     4981 gggccgaagg tggtgacgtc gaggtcggcg ccggacacga tgtcggacac cgctgccgca
     5041 acggcgtggc gggtgcccac caactccgcc atgacgaccg ccgggggcag cccgagctcg
     5101 gcccgctcgg tcaactcacg ctcggcgagc cacaccggat cccacctgac gagggcttcg
     5161 acggcgggga acggtgggcc gtcggggacc cccgcgagaa caacgacccc accgtccagc
     5221 gccgaccgac agagggcggc ggccgccagc caccgacgca acgcctcgac gggggcgtcc
     5281 aactcgggcc gactcaaggt ggcccaggca tccagcagca ggcacgcggc atacccctgg
     5341 gtcgctcgcg gctccgcccc cggcgtcgcg atgaccagcg ccggggtggc cgccacctga
     5401 cccgccacct caccggatgc cgaggtgatg accgggacgc cagggaaggc tcgtcccagc
     5461 tcctccgccg tcctgcggct gccgaggatc gccgcgcgcc aggccgtgct gtcgcactgc
     5521 ggacagcgga acccggcgac accgaatccc gtgccgcacc acgtgcaggc cggagggtgg
     5581 cgtggatcgt cctgacccat cgggccgtga caggcggtgc agcgcaccgg tgctcggcag
     5641 acctggcagg acagcgaggg gcggtaaccg ccgcggggca cctggacgag caccgggccg
     5701 cgggtcaacc cctcgtggac gatccgccag gcactcggtg gcaactgagc actggcagca
     5761 gcgctgtcgc gctcccgccc gacctcccct tcgccggcga cgacgacacg cgcggctgct
     5821 gcacggaggg tcgccggggt g
//
LOCUS       DAOMZY010000231         5827 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_12952_length_5827_cov_6.175502, whole genome shotgun sequence.
ACCESSION   DAOMZY010000231 DAOMZY010000000
VERSION     DAOMZY010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12952_length_5827_cov_6.175502"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PLK64_12285"
     CDS             complement(<1..302)
                     /locus_tag="PLK64_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPV80571.1"
                     /translation="MAEARSLPRVLVVTGTNTGVGKTVATAALAVALRARGLRVAVAK
                     PTQTGVSADEPGDLQEVCRLAGAVGLTAWEGVRLRDPLAPASAARTEGRSIPAVAD"
     gene            complement(295..1407)
                     /locus_tag="PLK64_12290"
     CDS             complement(295..1407)
                     /locus_tag="PLK64_12290"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016996168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HPV80572.1"
                     /translation="MTDFLDWLAVAAATRAERGLVRSTAVSAPGLLDLAGNDYLGLRV
                     HPRVIAGAVEAATAYGGGAGASRLVTGTLPLHGELESALATLTGSPAGLVFSTGYAAN
                     LAVMTALADADTLVVSDAHVHASLVDGSRLSRAAVAIARHNDVAHVRELLGGRPQRRA
                     VVVVESVYSVLGDAAPLADLVEVCRAHGAILVIDEAHALGVVGDRGQGMAAELGVTDH
                     PDIVLTLTLSKSLGSQGGAVLAHPAVREHLVNTARSFIYDTGLAPAAVGAAMGALDVL
                     AEQPELAKVCRDNAAALAEACGVEQPAGAVLSRAMAGPDEALAAVDRAREGGIRIGCF
                     RPPSTPDGSSRIRLTAHADHADLGPALDVLREIVGG"
     gene            1632..2114
                     /locus_tag="PLK64_12295"
     CDS             1632..2114
                     /locus_tag="PLK64_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80573.1"
                     /translation="MNTIPSDSVDIPLPGDVTAAQARAALSQASSAQLVTPRDLRLLT
                     GFVAGVGVIVGAILYLSWATLSVGNPGGFAISMSLYAAALALLLVIKSRARAIPRGFR
                     RTYNIGFAVTMTAYCTGILWIFGHQSPWPSASVVIPMSIVTAVPCLIAAALVHRTARA
                     "
     gene            2111..2416
                     /locus_tag="PLK64_12300"
     CDS             2111..2416
                     /locus_tag="PLK64_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HPV80574.1"
                     /translation="MTDQEQHARFRLDEVIHAPVRFSIVAALARVDQADFAAVRDAVQ
                     VSDSVLSKQVTLLEKAGYVEVKKGYVGKRPRTWLQLTPAGESAFSEHLLALRAIAGQ"
     gene            complement(2435..3802)
                     /locus_tag="PLK64_12305"
     CDS             complement(2435..3802)
                     /locus_tag="PLK64_12305"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012244011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HPV80575.1"
                     /translation="MSDAGFEGLLAYDRDHVWHPYSSALAPSQPYFVESAAGVRLRLR
                     LPSGEVRECIDAMSSWWCAIHGYAVPELDTAARAQLGRMSHVMFGGLTHEPGIKLARR
                     LIDLAPRTTADGESPLQHVFFADSGSVSVEVAMKMALQFHVAAGRPRTRFFTIRGGYH
                     GDTFQPMSVTDPVGGMHSLFSGILPAHVFAPKPPPAWGTGNEDGAGGSESEREAALAE
                     WESATRALYAAHADSIAAVILEPILQGAGGMSFHDPRVVQVLADLAREHGALVIFDEI
                     ATGFGRTGTMWAADQCGVVPDIMCAGKALTGGYLTLAAVLCTHEVALGVSAGEAGGLM
                     HGPTFMANPLACAVALASLELLQRNDVHRQVAGLEAGLSAGLAPARDLASVADVRVRG
                     GVGVVQLREPVRVREVAAYAIERGVWVRPFRDLVYTMPPYVTSPDDLATVCRVLTDAV
                     AAVHG"
     gene            complement(3799..4077)
                     /locus_tag="PLK64_12310"
     CDS             complement(3799..4077)
                     /locus_tag="PLK64_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80576.1"
                     /translation="MGAHDGAPLASDSLAASDGLAESDSPVAVFCPRCGIPLEHGDHT
                     VCRAALALEPPRYCPTCRRRMVVQVTPSDWTARCVEHGETTRSTWAPA"
     gene            complement(4077..5090)
                     /gene="bioB"
                     /locus_tag="PLK64_12315"
     CDS             complement(4077..5090)
                     /gene="bioB"
                     /locus_tag="PLK64_12315"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HPV80577.1"
                     /translation="MTAEAVDTDILHVAREQVLERGERLTYDQIVQVLQTSDDQLDEL
                     LAIAHEVRMRFMGPDVEVEGIISLKTGGCPEDCHFCSQSGQFTSPVRSVWLNVPELVR
                     AAKLTRDTGASEFCIVAAVRGPDAKLMEQLREAVAAIRDSVDINVAASIGILTAEQAA
                     EMADMGIHRYNHNLEAGRSFFPQVVTTHSWEERWDTCQLIKDSGMELCCGGLVGMGES
                     IEQRAELADQLGELEPHEVPLNFLNPRPGTPFGDLPVMPANDALRCIAAFRLALPRTI
                     LRYAGGRELTLGDLGTRSGLLGGINAVIVGNYLTTLGRDPKEDLALLDDLKMPIKALT
                     DTF"
     gene            complement(5188..>5827)
                     /locus_tag="PLK64_12320"
     CDS             complement(5188..>5827)
                     /locus_tag="PLK64_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV80578.1"
                     /translation="AARWWPEGQGAAYDVGPVTLSIGAGEHLVLTGANGSGKSTLLAV
                     LARHLDPAAGSYCLGGVDVTTVPLAEIRAHVAVVDDEPHIFASSLRENLRLARPDADD
                     GAIEAAIRAAGLAGWFDSLTEGLDTMLGTGGRGVSGGERARLALARAHVSNRPVVLLD
                     EPVAHLDHATALAVLADAHRAFAGRTVVMVSHRPDGLDGFDRVVDLADLADT"
BASE COUNT          878 a         2076 c         2049 g          824 t
ORIGIN      
        1 tagtcagcca cagcagggat cgaccgcccc tcggtccgag ccgcgctcgc gggcgccagc
       61 gggtcgcgaa gccgcacccc ctcccacgcc gtcaacccga cagcgccagc gagacggcat
      121 acctcctgca ggtcgccggg ctcatccgcc gagacgccgg tctgggtcgg tttggccacg
      181 gccacccgca gcccgcgtgc gcgcaacgca acggccagcg cggcggtggc gacggtcttg
      241 ccgacccccg tgttcgtccc ggtgacgacc aacacgcgcg gcagcgaacg agcctcagcc
      301 accgacgatc tcccgcagca catccagcgc cggaccgagg tcggcatggt cggcgtgcgc
      361 cgtcagccgg atccgcgagg acccgtccgg ggtcgacggg ggccggaaac acccgatccg
      421 tatgccgccc tcccgcgccc gatcgaccgc cgccagcgcc tcgtcggggc cggccatcgc
      481 ccgagagagc acggctccgg cgggctgctc gacgccgcac gcctcggcca gcgcggcggc
      541 gttgtcgcgg cacaccttgg ccaactccgg ctgctcggcg agcacgtcga gggctcccat
      601 ggccgcaccg acggcggccg gcgccaggcc cgtgtcatag atgaacgacc gggcggtgtt
      661 gaccagatgc tcgcgcactg ccgggtgagc caggaccgca ccgccttgcg agccaaggga
      721 tttcgagagg gtgagggtga gcacgatgtc cgggtgatcg gtgacgccga gctccgcggc
      781 catgccctga ccgcgatccc cgacaacccc gagcgcgtgt gcttcgtcga tgacgaggat
      841 ggcgccgtgg gcgcggcata cctcgacgag gtcggccagg ggagcggcgt cgccgaggac
      901 ggagtagacc gactcgacga cgaccaccgc ccgccgctgg ggccgaccgc cgagcaactc
      961 gcgcacgtgg gcgacgtcgt tgtgtcgcgc gatggcgacg gcagcgcgcg agagtcggga
     1021 gccgtcgacg agcgaggcgt gcacgtgggc gtcggacacg acgagggtgt cggcgtcggc
     1081 gagcgccgtc atgacggcga ggttcgcggc ataccccgtg gagaagacca gccctgcggg
     1141 cgagccggtg agggtggcga gggccgactc caactcacca tgcaggggga gcgtgcccgt
     1201 caccaggcgt gaagcccccg cgccgccgcc gtatgccgtg gccgcctcga ccgcgcccgc
     1261 gatgacgcgc gggtggacgc gcaggccgag gtagtcgttg ccggcgaggt cgagcaggcc
     1321 cggcgcgctg accgcggtcg agcggaccaa gccgcgctcg gcccgggtcg ctgcggccac
     1381 agccaaccag tcaaggaagt cggtcacggg ggcgatcctc tcactgcggg cgctcgtcgt
     1441 gcggcccctc tgtgcggccc ctgtgtgcgg cccaacccag cggcccaacc ggcggcccac
     1501 ccagcggccc acccagcgcc ctccgtcggc cctccggact gtgcagggaa aatacttgac
     1561 aatatggaaa gtagttgcct aaggtttgag caccgggcaa ccccgctcgg ctccgcatct
     1621 cggaggctca cgtgaacacc atcccgtccg actctgtcga catccccctt ccaggcgacg
     1681 tcacggccgc gcaggctcgc gccgccctca gtcaggcgtc aagcgcgcaa ctcgtgaccc
     1741 ctcgtgacct gcgtctcctc accggcttcg tcgcaggtgt cggcgtgatc gtgggggcga
     1801 tcctctatct cagctgggcc actttgtcag tgggcaaccc gggcggcttc gccatcagca
     1861 tgtccctgta tgccgccgcc ctcgccctcc tgctcgtcat caagtcccgg gccagggcca
     1921 tccctcgagg gttccggcgg acctacaaca tcggcttcgc cgtgacgatg accgcctact
     1981 gcaccgggat tctgtggatc ttcgggcatc agtcgccgtg gccgagcgcg tccgtcgtca
     2041 tcccgatgag catcgtgacg gcagtgccgt gcctcatcgc cgcggcgctg gttcaccgga
     2101 cggcacgggc atgaccgacc aggagcagca cgcgcggttc cggctcgacg aggtgatcca
     2161 cgcgccggtt cgattctcga tcgtcgcggc gctcgcccga gtcgaccagg ccgacttcgc
     2221 tgcggtccgc gacgcggtgc aggtgagcga ctcggtcctg tccaagcagg tgacgctgct
     2281 ggagaaggct ggctatgtcg aggtgaagaa gggctatgtc ggcaaacggc cccgcacctg
     2341 gctccaactg actcccgccg gggagagcgc gttcagcgag cacctgctcg ccctgcgcgc
     2401 gatcgccggc cagtgacctc agcgccgacg accgtcagcc gtggacagcg gcgacggcgt
     2461 cggtgagcac gcggcatacc gtcgcgaggt cgtcggggga ggtgacgtag gggggcatcg
     2521 tgtagacgag gtcgcggaac ggccgcaccc acaccccccg ctcgatcgcg tatgccgcga
     2581 cctcccgcac gcgcactggc tcgcgcagtt ggacgacgcc aacacccccg cgcacccgga
     2641 cgtcggcgac cgacgccagg tcgcgagccg gcgccagtcc cgccgacagc cccgcctcaa
     2701 gccccgccac ctgacgatga acatcgttgc gttgcaacaa ctccaggctc gccagggcca
     2761 ccgcgcaggc cagcggattg gccatgaacg tcggcccgtg catcagccca ccggcctcgc
     2821 cggcactgac ccccaacgca acctcgtggg tgcacaacac ggcggcgagc gtgaggtagc
     2881 ccccggtcag cgccttcccc gcacacatga tgtcgggcac cacgccgcac tggtcggccg
     2941 cccacatcgt ccccgtccgg ccgaacccgg tcgcgatctc gtcgaagatc accagcgccc
     3001 cgtgctcgcg ggccaggtcg gccagcacct gcacgacccg ggggtcgtgg aagctcatcc
     3061 caccggcccc ctgcaggatc ggctcgagga tgaccgcggc gatggagtcg gcgtgggcgg
     3121 catacagggc tcgcgtggcg ctctcccact cagcgagggc cgcctcgcgc tcgctctcgc
     3181 tgccgcccgc gccgtcttcg ttccccgtgc cccacgcggg cggcggtttg ggggcgaaga
     3241 cgtgcgcggg gagtatgccg ctgaacaggc tgtgcatccc gcccaccggg tcggtgaccg
     3301 acatgggttg gaaggtgtcg ccgtgatagc cgccgcggat cgtgaagaag cgagtgcgtg
     3361 gccgtccggc cgcaacatgg aactgcagag ccatcttcat ggcgacctcg accgagacag
     3421 agcccgagtc ggcgaaaaac acatgctgga gcggggattc accatcggct gtggtcctcg
     3481 gagccaggtc gatgagccgc cgagccagct tgatccccgg ttcatgggtg agcccgccga
     3541 acatcacgtg cgacatgcgc cccagctgag cccgcgcggc ggtgtcgagc tcgggcacgg
     3601 catacccatg gatcgcgcac caccacgagg acatggcgtc gatgcactcg cggacctcgc
     3661 cgctagggag tcgcagccgc agccgcacgc ccgccgccga ctcgacgaag tagggctggg
     3721 acggggcgag cgcggaggag taggggtgcc agacgtggtc gcggtcgtag gcgagcagcc
     3781 cctcgaaacc cgcgtcgctc acgccggcgc ccacgtgctg cgggtcgtct ccccgtgctc
     3841 gacgcagcgc gccgtccagt ccgacggggt cacctggacg accatccggc gccggcacgt
     3901 ggggcagtag cgcggcggct caagggcgag cgccgcgcgg cataccgtgt ggtcaccgtg
     3961 ctccagaggt atgccgcatc gcgggcagaa caccgcgacc gggctgtccg actcggcgag
     4021 gccgtcagat gcggccaggc tgtcggaagc gagcggagcg ccgtcgtgcg cacccatcag
     4081 aacgtgtccg tgagggcctt gatcggcatc ttgaggtcat cgagcagcgc gaggtcttcc
     4141 ttggggtcgc ggcccagcgt tgtgaggtag ttgccgacga tgaccgcgtt gatgccgccg
     4201 agcaatccgc tgcgggtgcc gaggtcgccg agggtcaact cgcgcccacc ggcataccga
     4261 aggatcgtgc ggggcagcgc gaggcggaag gcggcgatgc agcgcaaggc gtcgttggcc
     4321 ggcatgacgg ggaggtctcc gaacggcgtc ccggggcgcg ggttgaggaa gttgagcggc
     4381 acctcgtgcg gctccaactc gcccaactgg tcggcgagtt cggcgcgctg ctcgatgctc
     4441 tcgcccatgc cgacgagccc gccgcagcac agctccatgc cgctgtcttt gatgagttgg
     4501 caggtgtccc aacgctcttc ccacgagtgg gtggtgacca cctgggggaa gaacgagcgg
     4561 ccggcctcga ggttgtggtt gtaacggtgg atgcccatgt cggccatctc ggccgcctgc
     4621 tcggcggtga ggatgccgat cgacgcggcg acgttgatgt cgacgctgtc gcggatcgcg
     4681 gcgacggcct cgcgcagctg ctccatgagc ttggcgtcag gcccgcgcac ggcggcgacg
     4741 atgcagaact cgctggcgcc cgtgtcgcgg gtcaacttgg ccgcgcggac caactcgggc
     4801 acgttgagcc acaccgaacg caccggcgag gtgaactgcc ccgactgcga gcagaagtgg
     4861 cagtcctcgg ggcaaccgcc cgttttgagg ctgatgatgc cctcgacttc gacgtcgggc
     4921 cccatgaacc gcatccgcac ctcgtgcgcg atggcgagca actcgtcgag ctggtcgtca
     4981 ctggtctgca ggacctgcac gatctggtca taggtcagcc gctcgccgcg ctcgaggacc
     5041 tgctcgcgcg ccacgtggag gatgtcggtg tccactgcct ctgccgtcat gaccgcgatt
     5101 cttccgtatg ccgcgcccgc tcgctcccag ccgttggtcc cgaaccgttg tcgggggtca
     5161 gttgacctcg atttcgggcc cggccagtca ggtgtcggcg aggtcggcca ggtcgacgac
     5221 tcggtcgaag ccgtcgaggc cgtccgggcg gtggctgacc atgacgacgg tgcgcccagc
     5281 gaaggctcgg tgcgcgtcgg ccaacacggc cagcgctgtg gcgtggtcca ggtgcgcgac
     5341 cggttcgtcg agcaggacga ccggacggtt gctgacgtgg gcccgcgcaa gagccagcct
     5401 ggcgcgttcg cctccggaca cgccgcgccc gccggtgccg agcatggtgt ccaggccctc
     5461 ggtgaggctg tcgaaccatc cggcgagccc ggcggcccgg atggcggcct cgatcgcgcc
     5521 gtcgtctgcg tcgggccggg cgaggcggag gttctcgcgc agggacgacg cgaagatgtg
     5581 tggttcgtcg tcgacgacgg ccacgtgggc gcggatctcg gccagcggca ccgtggtgac
     5641 gtcgaccccg ccgaggcaat agctgcccgc cgccgggtcc agatggcgcg ccagcacggc
     5701 gagcagggtc gacttgccgc tcccgttggc gccggtgagg acaagatgct ccccggcacc
     5761 gatgctcaaa gtcaccggcc cgacgtcgta tgccgccccc tggccctctg gccaccaccg
     5821 ggcggcc
//
LOCUS       DAOMZY010000232         5812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_12993_length_5812_cov_5.907243, whole genome shotgun sequence.
ACCESSION   DAOMZY010000232 DAOMZY010000000
VERSION     DAOMZY010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12993_length_5812_cov_5.907243"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /locus_tag="PLK64_12325"
     CDS             complement(<1..1320)
                     /locus_tag="PLK64_12325"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPV80579.1"
                     /translation="MIDTFITTVMRRARAILITSLVILVAAAALGVGAISRLQSGGFD
                     DPSAESAQAATALAEKLGRPTANFLLLVTAPSGATVDDAVVADVGRAAVSRLDAEPGV
                     EAVADFWSAPAGAAAALRGSGGRTALVVAHIDGDEDDYRERIATLQPAYTTTSPEGVT
                     VQTGGAAQTVTDITLQVKKDFALAEALAIPATLLLLVVVFGSFIAGLLPMLVGAYAMV
                     GTLAVLRILTAVTDVSIFSLNLTTALGLGLGIDYALLVVNRFREELQSGDDVEGALRA
                     TMHTAGRTVAYSAITVAVALGALLAFPMYFLRSFAYAGIAVTLLTAGVSLVVLPAALR
                     LLGHRAAYRRFGKPTRYAATGLWSRVATVVMRRPVLTAAPVVVLLGVLAAPLAGVAFG
                     LPDDRSIPVSHSQGRAVAEVLRTDFTSRENEAITVVFPDGAPERTDRP"
     gene            complement(1367..1912)
                     /locus_tag="PLK64_12330"
     CDS             complement(1367..1912)
                     /locus_tag="PLK64_12330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV80580.1"
                     /translation="MPWLTPALPAFSPATHPYLTIRPLAPLEAGPLQAVFQGLSAKSR
                     QLRYHTPVSVLRPAMLRHLTDVDPGRHVAVVATFGGRPVGIGRWLRDPLRPTEAELAV
                     EVIDRCHGLGIGTALAAEAARLAVHAGVDTLVAHIDSANTAARGWARALGAAPHPDDP
                     EQVRIAAAALAPDIPRHHPAA"
     gene            2033..>3378
                     /locus_tag="PLK64_12335"
     CDS             2033..>3378
                     /locus_tag="PLK64_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HPV80581.1"
                     /translation="MPSLTRAEATRRAAQLAVQGYAVALDLDVGDERFASLTTVTFQA
                     LADGVQTWVDVKATDVHEVLLDGIPVDLAGWRDGRLALPALAGGAHTLSVDATMAYRR
                     DGSGLHRAVDPADGEAYVYVQSFLDYAPAVFACFDQPDLKAPISLRVHAPAHWIIVGN
                     GAATQVGDGEWELAATAPISTYLFTVCGGPFVSVRDSHDGIPLGLHARASMRAELERW
                     APQIFEVTKQSFDAYHALFGIRYPFGDYHQVFVPDFNAVAMENPGCVTLRDALLYRGA
                     ATPDEVLRRSRTVCHEMAHMWFGDLVTMKWWDDLWLNESFAEYLAHRVLTGATEFADA
                     WVDFGILRKPWGYAAERAPSAHPVAGAPALTAEDALSDFDGISYAKGATALRQLIAFV
                     GDEVFVGGVSNYLRDKAFGNGTFAEFLAAIEASAGRDLGDWARDWLLTTGRDTFAV"
     assembly_gap    3379..3478
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3479..4596
                     /locus_tag="PLK64_12340"
     CDS             <3479..4596
                     /locus_tag="PLK64_12340"
                     /inference="COORDINATES: protein motif:HMM:NF023266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ERAP1-like C-terminal domain-containing protein"
                     /protein_id="HPV80582.1"
                     /translation="VLTRRAPAAYPASRPHVLDVGVYAASAPPEVTRVLADGERTALP
                     DLRVALGDRLVLPNCSDLTWASIDFDEATLAALPARLGELADPLARSVCWQSLDDGVA
                     RGVVDPRLFLDAIARSWPRETHPALFANVADDALVVLSRFVPDDLRPGLRAELAETAR
                     TVIEVEPDGSPRATIAARVVARASTHEGLLRTWLDAGPSWLQGDTDFRWLVVGNLAGL
                     GRLREEELAAAEAADPTVSGRLAGLLARASVPTVAAKTWAFEQLVDPASGHTNHALVE
                     LARGLWRSPDRGLVRPFVEPFLDAIPRLTAWVGDDALTKVVRFGFPFVVEARTIELVD
                     AALSRDDLTPGVRRAFVEWSWPVREALASRSRWFPTG"
     gene            4727..>5812
                     /locus_tag="PLK64_12345"
     CDS             4727..>5812
                     /locus_tag="PLK64_12345"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPV80583.1"
                     /translation="MSPAQHAAAVRALFLNYEHLDPDVPVRLAKRTTNLFRPRAAAMG
                     PGLDVSALTGVIDVDPVAGTATVQGMATYESVVDATLAAGVMPLVVPQLRTITVGGAV
                     TGLGIEASSFRNGLPHESVRSMDILIGTGEIVTAEPTGPHADLFRTFPNSYGSLGYAV
                     SLTIDVEPVAPFVRLRHLPFDSLRSLTEAVGQIVAMRAWDGEPVDFLDGVVFARNAAY
                     LTLGRFEQTLDAAETPSDYAGQRIFYRSIRERSTDVLTVRDFLWRWDTDWFWCSRAFG
                     VQQPLVRRLWPRRLKRSDVYQRIVGLENRHGVAARVDSWRGRPARERVVQDVEIPLER
                     TADFLDWFLREVPIEPIWLCPVALRESG"
BASE COUNT          786 a         1849 c         2171 g          906 t
ORIGIN      
        1 cggacggtcc gtgcgttccg gggctccgtc ggggaagacg acggtgatcg cctcgttttc
       61 gcggctggtg aagtcggtgc gcagcacctc ggccaccgcc cggccctgcg agtggctcac
      121 cgggatggac cgatcgtcgg gcagcccgaa cgccacgcca gccaggggcg cggcgagcac
      181 accgagcagg accaccaccg gggccgcggt gagcactggg cgacgcatga cgacggtggc
      241 aacgcggctc cacaggccgg tggcggcata cctggtgggt ttgccgaagc gccggtatgc
      301 cgcgcggtga ccgagcagtc gcagtgccgc gggcaggacg acgagggaca caccggccgt
      361 gagcagggtg acggcgatac cggcatacgc gaaggaccgg aggaagtaca tcgggaaggc
      421 gagcaacgct ccgagggcga ccgcgaccgt gatcgcggag taggcgacgg tccgtccggc
      481 ggtgtgcatc gtggctcgga gcgctccctc gacgtcgtct ccggattgga gttcctcgcg
      541 gaaccggttg acgacgagca gcgcgtagtc gatgccgagc ccaagcccga gagcggtggt
      601 gaggttgagg gagaagatgc tgacgtcggt gaccgcggtg aggatgcgca ggacggcgag
      661 ggtgccgacc atggcatagg cgcctaccag catgggaagc aatccggcga tgaacgaccc
      721 gaacaccacg acgagcagca gcagggtggc ggggatggcg agggcttcgg cgagggcgaa
      781 gtccttcttc acttgcaggg tgatgtcggt gacggtctgg gcggcgccgc cggtctggac
      841 cgtgaccccc tcgggagagg tcgtcgtgta ggccggttgg agggtggcga tgcgttcgcg
      901 gtagtcgtcc tcgtcgccgt cgatatgggc caccacgagg gcagtgcgcc caccggagcc
      961 acgcagcgcg gccgcagcac ccgccggtgc gctccagaag tcggcgacgg cctcgacccc
     1021 tggttcggcg tcgaggcgac tgacggctgc tcggccgaca tccgcgacga cggcgtcgtc
     1081 caccgtcgcg cccgaggggg ccgtgacgag aaggaggaag ttggcggttg gacggccgag
     1141 tttctcggcc agcgccgtgg ctgcctgggc agactcggct gacggatcgt cgaagccgcc
     1201 cgactgcagc cggctgatgg cgccgactcc gagcgccgcg gcagcgacga ggatgaccag
     1261 gctggtgatg aggatcgcgc gagcccggcg catgacggtg gtgatgaagg tgtcgatcat
     1321 ggggagagtc cttcgtgggt ttgatgcggg gaagctgatg gaccggtcag gcggccgggt
     1381 ggtgtcgggg gatgtcgggg gcgagcgcgg ctgctgcgat gcggacctgt tcgggatcgt
     1441 ccgggtgagg ggcggcgccg agggcccggg cccaaccgcg cgccgccgtg ttggccgagt
     1501 cgatgtgcgc gacaagggtg tcgacccctg cgtgtacggc cagccgggcg gcctccgcgg
     1561 cgagcgcggt gccgatcccc agcccatggc agcggtcgat gacctcgacc gcgagctcgg
     1621 cctcggtcgg ccggagcggg tcccgcagcc agcgtccgat gccgaccggt cgcccaccga
     1681 aggtggccac gacggcgacg tggcgtccgg ggtcgacatc ggtgaggtgg cgcagcatcg
     1741 cgggtcgcag gaccgacacg ggggtgtggt agcgcagctg gcgcgatttc gcgctgagcc
     1801 cctggaagac ggcctgcagc ggaccggcct ccaggggggc caggggtcgg atcgtcaggt
     1861 agggatgagt ggcgggggag aaggccggaa gggccggggt gagccagggc atggggagag
     1921 ctccttgtga gtgctgacgt tcgccgatgg tggggtgagg ccctgccgcg cccctgtcac
     1981 cggtctgtca cgtccccggc ggacccggat gtcgggtggc agactcgcgg gtatgccgtc
     2041 tctcacccgc gccgaagcga cacgtcgcgc tgcgcagttg gccgtgcagg ggtatgccgt
     2101 ggcgctggat ctcgacgttg gcgacgaacg tttcgcgtca ctgacgacgg tgaccttcca
     2161 ggccctggcc gacggggtgc agacctgggt cgacgtcaag gcgaccgacg ttcacgaggt
     2221 gctgctggac ggcataccgg tcgaccttgc ggggtggcgc gacgggcgac tcgcgctgcc
     2281 cgccttggcc gggggtgccc acacgctgag cgtcgacgcg acgatggcct accggcgcga
     2341 cgggtcgggg ctgcaccggg cggtcgaccc ggccgacggg gaggcctatg tctatgtgca
     2401 gtcgttcctc gactatgcgc cggcggtgtt cgcctgcttc gaccagcccg acctcaaggc
     2461 cccgatcagc ctgcgggtgc acgctccggc gcactggatc atcgtcggca acggcgctgc
     2521 gacgcaggtc ggtgacggtg agtgggagct cgcggccacg gcgccgatct cgacctatct
     2581 gttcaccgtg tgtggcggtc ccttcgtctc cgtgcgggat tcgcacgacg ggatcccgct
     2641 gggcctgcac gcacgggcat cgatgcgtgc cgagttggag cgctgggcgc cacagatctt
     2701 cgaggtgacc aagcagtcgt tcgacgccta tcacgcgctc ttcgggatcc ggtatccctt
     2761 cggcgactat caccaggtgt tcgtcccgga cttcaacgcg gtcgccatgg agaacccggg
     2821 gtgcgtgacg ttgcgcgatg cgttgctcta tcgaggggcg gccacgcccg acgaggtgct
     2881 gcgccgctcg cggaccgtct gtcacgagat ggcccacatg tggttcggcg acttggtcac
     2941 catgaagtgg tgggacgacc tgtggctgaa cgagtcgttc gcggaatacc tcgcgcaccg
     3001 ggtgctcacc ggcgcaaccg aattcgccga cgcctgggtc gacttcggca tccttcgcaa
     3061 accctggggg tatgccgcgg agcgcgcacc gtccgcccac cccgtcgcgg gggcgccggc
     3121 gctgacggca gaggacgccc tgtcggactt cgacgggatc tcctatgcca agggcgccac
     3181 cgcgttgcgg caactcatcg ccttcgtggg agacgaggtc ttcgttggtg gtgtctcgaa
     3241 ctacttgcgg gacaaggctt ttggcaacgg aaccttcgcc gagtttctgg cggcgatcga
     3301 agcgtccgcg ggccgtgacc tgggcgactg ggccagggac tggctgctga cgacgggccg
     3361 ggacaccttc gcggtcgann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg
     3481 gtgctcacgc gacgggcgcc cgcggcatac cccgcctccc gcccgcacgt gctcgacgtc
     3541 ggtgtgtatg ccgcgagcgc tccacccgag gtgacccgcg tcctggccga cggggagcga
     3601 acggcgttgc cggacctgcg ggttgccctg ggcgaccggc tggtcctgcc gaactgcagc
     3661 gacctcacgt gggcgtcgat cgacttcgac gaggccacgc tggctgccct gccggcccgg
     3721 ctgggggagt tggccgaccc gctggcccgc tcggtctgct ggcagtcctt ggatgacggc
     3781 gtggcccggg gagtcgttga cccacggctg ttcctcgatg cgatcgcgcg gtcgtggccg
     3841 cgggagacgc atcctgcgct gttcgccaac gtcgccgacg acgccttggt ggtcttgtcg
     3901 cgtttcgttc ctgatgacct gcgaccgggg ctgcgtgcgg agctggccga gaccgcgagg
     3961 accgtcatcg aggtggagcc ggacgggtcg ccccgggcga cgatcgcggc ccgggtcgtg
     4021 gcccgggcgt cgacccacga ggggctgctg cggacctggc tggacgccgg gccgtcgtgg
     4081 ttgcagggcg acaccgactt ccggtggctc gttgtcggca acctcgctgg cctgggacgc
     4141 ctgcgcgagg aggagttggc ggccgccgaa gccgccgacc cgacggtgtc cgggcggctg
     4201 gccgggttgt tggcgcgggc ctcggtgccg acggtggccg ctaagacctg ggcgttcgag
     4261 caactcgtcg atcccgcgtc gggtcacacc aaccatgccc tcgtcgagtt ggcgcggggc
     4321 ctgtggcgct cgcccgaccg agggctcgtt cgcccgttcg tcgagccgtt cctcgacgcc
     4381 attccgcggt tgaccgcatg ggtgggggac gacgcgctga cgaaggtggt gcgcttcggc
     4441 ttcccgttcg tcgtcgaggc gcgcaccatc gagctcgtcg acgccgcgtt gtcccgcgac
     4501 gacctgaccc ccggcgtgcg tcgggccttc gtcgagtggt cgtggccggt gcgggaggcc
     4561 ctggcgtcgc gcagccgctg gttccccacc ggctgacgcg agggcggcgc gccgccaatc
     4621 ggggtgtgcg gcatacccac tggtaactgc ccatggcagg gtccagagag ctagcctcgg
     4681 atggagccac ccgccggcga cgacgcgagg aggtgaaagc gcggtggtgt cacctgcgca
     4741 gcacgcagcc gccgtgcgcg cactcttcct caactacgag cacctcgacc cggacgtccc
     4801 cgtgcggctg gccaagcgga cgaccaacct gttccgcccg cgggcggccg cgatgggtcc
     4861 gggcctggat gtgtcggcgc tgaccggggt gatcgacgtc gacccggtgg ccgggaccgc
     4921 gacggtgcag gggatggcga cctacgagag cgtcgtcgac gccaccctcg cggccggggt
     4981 catgccgctc gtcgtgccgc agttgcgcac catcaccgtg ggcggtgccg tgacgggcct
     5041 ggggatcgag gcgagcagtt tccggaacgg gctgccgcac gaatcggtcc gctcgatgga
     5101 cattctcatc ggcacgggcg agatcgtcac cgcggagccg acgggcccgc acgccgacct
     5161 gtttcgcacc ttccccaact cctacggctc cctcgggtat gccgtgtctc tcaccatcga
     5221 cgtcgagccg gtggcgccgt tcgtccggct gcggcacctg ccgttcgact cgttgcggtc
     5281 gctgaccgag gcggtcgggc agatcgtcgc gatgcgtgcc tgggacggcg agccggtcga
     5341 cttcctggac ggggtcgtct tcgcgaggaa cgcggcatac ctcacgttgg ggcgtttcga
     5401 gcagactctc gatgccgccg aaacgccgag cgactatgcg ggacaacgga tcttctaccg
     5461 ctcgatccgc gagcggagca ccgatgtgct caccgtgcgc gacttcctgt ggcgctggga
     5521 caccgattgg ttctggtgct cgcgggcatt cggagtgcag cagcctttgg tgcgacggct
     5581 gtggccgcga cggctcaagc gctcggatgt ctatcaacgc atcgtcgggc tcgagaaccg
     5641 ccatggggtg gcggcgcgcg tcgattcgtg gcgcggcaga cccgcgcgcg agcgggtggt
     5701 gcaggacgtc gagatcccgc tggagcggac cgctgacttc ctcgactggt tcctgcgcga
     5761 ggtgccgatc gagccgatct ggttgtgccc cgtggccctt cgtgagtcgg gc
//
LOCUS       DAOMZY010000233         5808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_13006_length_5808_cov_5.165305, whole genome shotgun sequence.
ACCESSION   DAOMZY010000233 DAOMZY010000000
VERSION     DAOMZY010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13006_length_5808_cov_5.165305"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..923)
                     /locus_tag="PLK64_12350"
     CDS             complement(57..923)
                     /locus_tag="PLK64_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPV80584.1"
                     /translation="MSALAIGLLLGAGVFCIFWSTWPQTERSAPARRASLADRVRDQL
                     VQAGFDTISPGQLLAACMVVFVIVFLGAYSMTRVVPIAICFAAMAGYGPRALVTMRAR
                     KRRGQLRDLWPDAVDNIASAVRAGLALPEALSQLAVRGPEELRPAFAAFAEDYRTTGR
                     FHDCLNRLKDRLADPVADRLVESLRIAREVGGSDLGRVLRTLSAFLREDARTRAELET
                     RQGWTVNAARLAAGAPWVVLAMMSIQQDSLRAYSSAAGTAVLAVGAAITVVAYRLMVR
                     IGRLPEDERVLR"
     gene            complement(923..2152)
                     /locus_tag="PLK64_12355"
     CDS             complement(923..2152)
                     /locus_tag="PLK64_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPV80585.1"
                     /translation="MADAVRQVEGEVRELIRRSGLDPARHPMEIDRLVRDAVTDYDER
                     SMRSGMPPLADLAGAAKSILDTVAGYGPLQRYFDDPKVEEIWVNSPDRVFIARAGQSE
                     LTNTILTADEVRDLVERMLRSSGRRVDLSSPFVDATLPDGSRLHVVIPDITRDHWHIN
                     VRKFVVRADHLDDLVRLGTLTRPAATFLEAAVVSGLNILVAGGTQAGKTTLLGCLASA
                     IPARERVVTCEEVFELKIPLRDVAGMQCRQPSLEGTGAVPLRRLVTEALRMRPSRIIV
                     GEVRQAESLDLLIALNSGLPGMCTLHANSAREAITKMCTLPLLAGENVSPRFVVPTVA
                     SSVDLVVHIALEGDGTRRVREIVAVPGRVEGDVVEVADIFTTRGGVLKRADGFPPHVD
                     RFERAGYRLTALLAQEA"
     gene            2341..>5808
                     /locus_tag="PLK64_12360"
     CDS             2341..>5808
                     /locus_tag="PLK64_12360"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HPV80586.1"
                     /translation="MAGRAVEVRVAARRGATFAELLDALDAAHALTDTSRTWSVDSVG
                     VPPTATLGLPPLLDGARLMIRPAGSTSPDTSTASAASGLLQLHAVGGPDSGLVHDLPP
                     GRHVLGRGANTPIRVGDSSLSRLHAEITVAGRSITVRDLESTNGTWVNGIPVGGEPIT
                     LAAGATLQTGDSRWQLRTPATVSAATRPDHEGHLLVSRSPRLPLPGPAAPIPFPEEPN
                     PPAGQRLPIAAATLPLVFSAVLAVVMQSPTMLLFGLMGPLLLLGSWWSDRRNGKRVTR
                     AEQRRYAAALATARALLTAALAAEREVRLAEDPDPAWLAGLARGPLTGLWDRTGDAAL
                     RVRIGLGTICARQSVAGSPHPPTMDEVPVVIRLDGGAATGIIGPREEALRLARWMIGQ
                     LSLRYSPADLSIGLLAPESEREWSWVAELPHSQAEAERDAGRGRGVILVDGTPAPAPT
                     ENVHVLAVAADAADLPSACTQIVELRAGTSSLTWAQQPVTGLEPDGVPRAWALLLAQR
                     LGPLRDAAATPHKGVIPSRTTLLDVLEIPERDGIPDLTARWRESPRSTLTPIGATASG
                     PLPIDLVRDGPHALVGGTTGSGKSELLVSLVAGLAAANRPDELSFVLVDYKGGAAFGA
                     CRDLPHVVGLVTDLDHQLTERALVSLDAELKRRERVFGEHGVADLAAYQGVCRPGDPT
                     VGRLVIVVDEFRSLAEELPEFVTGLVRVASLGRSLGVHLVIATQRPGGVVTADMRANL
                     GLRIALRVRDIVDSLDVVESPVAASIAESTPGRAILTSAATRLVEVQTALATGPSRRA
                     VEPPVRVVSIDGLAVPQSVRALDGPTQVDDLVAASRRAVLELSIPLPGSPWLPPLSGD
                     LTVASLAGGSGWPVPLGRRDVPLHQRQSDWTWDPLREGHLGIVGAPRTGRTTALLTVA
                     GQLAARFSPDEIHLYAVHAGSLGHLDALPHVGASVRCDELPRLAQLLTILTDPSASTR
                     ASSSTSSSSSETMRVLLVDDWDRVCEELDRARAGVLRDRLTALLRRGPRERLALCLTG
                     GRASLSGSIGQLVPRRLLLLPAEPVDLAMAGLSARAVPQHPTPGRGIDLADGSEVQVA
                     SVVAEPTAARCTAYLEELARNLPAREGDSPPPTVPTLPAEVSLSALQHPPGLLVVGSD
                     GREATGFDLA"
BASE COUNT          871 a         2101 c         1941 g          895 t
ORIGIN      
        1 gaccgagaag agcgcacccg ccgccgcccc ggccatcgac cacccgccca cgccactcat
       61 cgcagcaccc gctcgtcctc gggaagtcgc ccgatccgca ccatcaagcg gtaggcgacc
      121 accgtgatcg ctgcgccgac ggcgaggacc gccgtgcccg cggcactgct gtaagcacgt
      181 agcgagtcct gctggatcga catcatcgcc aggacaaccc agggggcacc tgctgccagg
      241 cgagcggcgt tgaccgtcca cccctgacgg gtctccaact cggcgcgggt gcgggcgtct
      301 tcacgcagga aggccgacag cgtgcgcagc acccgcccga ggtcagatcc accgacctcc
      361 cgggcgatcc gcagtgattc cacgaggcgg tcggcgaccg ggtcggcgag ccggtccttg
      421 agccggttga ggcagtcgtg gaagcgcccc gtcgtgcggt agtcctccgc gaacgcggcg
      481 aacgccgggc gcagttcctc aggtccgcgg acggcgagct ggctcagcgc ctcgggaagg
      541 gcaagtcccg cccggaccgc ggaggcaatg ttgtcgaccg cgtcgggcca caggtcacga
      601 agttgccccc gtcgtttgcg ggctcgcatg gtcaccaacg cccgcggccc gtaccccgcc
      661 atcgctgcga agcagatcgc gataggcacc acccgggtca tcgagtaggc accgaggaag
      721 acgatgacga agaccaccat gcaggcggcg agcaactggc cgggcgagat ggtgtcgaac
      781 ccggcctgga caagttggtc ccgcacccga tcagccaggc tggcccgacg cgcgggagcc
      841 gaacgctcgg tttgcggcca ggtcgaccag aagatgcaga agaccccggc gccgagcagc
      901 aggccgatcg caagagccga catcaggcct cctgcgccaa cagggcagtc agtcggtagc
      961 cggcccgctc gaatcggtcg acatggggcg ggaagccatc ggcgcgcttg aggacgccac
     1021 cgcgggtggt gaagatgtcg gccacctcga ccacgtcgcc ctcgacccgg ccgggcacgg
     1081 cgacgatctc ccgcactcgg cgggtcccgt caccttcaag ggcgatgtgc acgacgaggt
     1141 cgaccgaact ggccaccgtg ggcacgacga accgggggct gacgttctca ccggcgagca
     1201 gcggcagcgt gcacatcttg gtgattgctt cccgcgccga gttggcgtgc aaggtgcaca
     1261 tcccgggcaa ccctgagttg agggcgatca gcaggtcaag gctttcggcc tgacgaacct
     1321 cgccgacaat gatccgcgac gggcgcattc gcaacgcctc agtcaccaag cggcgcaacg
     1381 gaacagcgcc ggtgccctcc aggctcggct gtcgacattg cattcccgcg acgtcccgca
     1441 acgggatctt gagctcgaag acctcttcac acgtgaccac gcgttcccgc gccggaatgg
     1501 cggaggccag gcaccccagg agggtggtct tccccgcttg cgtgcctccg gcgaccagga
     1561 tgttcagccc ggagacgacc gccgcttcca agaacgtggc ggccgggcgg gtgagggtcc
     1621 cgaggcggac caggtcgtcc aggtggtcgg cccgcacgac gaacttccgc acattgatgt
     1681 gccagtggtc gcgggtgatg tcggggatga cgacgtgcag tcggctgccg tccggcaacg
     1741 tcgcatcgac gaacggcgag ctcagatcga cccgtcgccc ggaggaccgc aacatccgct
     1801 cgaccaggtc acgcacctca tcagcggtga ggatggtgtt ggtcaactcg gactgccccg
     1861 cgcgggcgat gaatacccga tcgggactgt tgacccagat ctcctcgacc ttggggtcat
     1921 cgaagtagcg ctgcaacggc ccatatccgg cgaccgtgtc gaggatggat ttggccgctc
     1981 cggcgagatc ggccaagggc ggcatgccgc tgcgcatgga gcgctcgtcg tagtcggtga
     2041 ccgcgtcccg caccagccgg tcgatctcca tgggatgacg ggccggatcg agcccggatc
     2101 gacggatgag ttcacgaacc tcaccctcga cctgacgcac tgcgtcggcc atcacgcctc
     2161 cgttcccctg agccttcttg aggcaaacat agtgattcgc ggcattcgcg catctcgtac
     2221 cggtacgtcc ctgtggataa cttctgcgcc cgtcggctcc ggtcgactac acatccggta
     2281 tgccgtgcat ccacatcagg gggaaattcc gtgcaccttc ggttgactgt ggtgaacccg
     2341 atggccggcc gtgccgtcga ggtgcgggtc gcggcccgcc gcggggcgac cttcgcagag
     2401 ttgctcgacg cgctcgacgc cgcgcacgcg ctcaccgaca ccagccgcac gtggtcggtt
     2461 gattcggtcg gtgttccccc cactgcgacc ctcggtctgc cgccgttgct cgacggagcc
     2521 cgactgatga tccgtcccgc agggtcgacc tcccccgaca cctcgaccgc ctcggccgcc
     2581 tcgggcctgc tgcaactcca tgccgtcggc gggcctgaca gcggcctggt ccacgacctc
     2641 cctccggggc gacacgtgct cggacgcgga gcgaatactc ctatccgcgt gggtgattcg
     2701 agcttgtctc gcctgcatgc cgagatcacc gtcgccggtc gatccatcac cgtccgcgac
     2761 ctggagtcaa caaacgggac ctgggtgaac ggcatacccg tggggggtga acccatcacc
     2821 ctcgcggccg gggcaacctt gcagacgggc gattctcggt ggcagttgcg cactccggca
     2881 acggtttccg cggcaacacg ccctgaccat gaaggacacc tgctggtcag ccggagccca
     2941 cgcctgccgc tccccgggcc ggccgcaccg atccccttcc ccgaggaacc gaacccacct
     3001 gcgggacagc gacttccgat cgccgccgcc accctcccgc tcgtcttctc cgcggtgctg
     3061 gcagtggtga tgcagtcccc cacgatgctg ctcttcggcc tcatggggcc cttgctgctc
     3121 ctcggctcct ggtggtcaga tcgtcgcaac gggaagcggg tcaccagagc agaacagcgc
     3181 cggtatgccg ccgcgctcgc caccgcgcgg gccctcctga ccgccgcctt agccgccgaa
     3241 cgtgaggtgc ggttggccga ggaccccgac ccggcctggc tcgcgggcct cgccaggggg
     3301 ccgctgaccg gcttgtggga ccggaccggc gacgctgcgc ttcgggtgcg gatcgggctg
     3361 gggacgatct gcgccagaca gtcggtggcc gggagtcccc atccgcccac gatggacgag
     3421 gtgcccgttg tcatccggct cgacggcggt gctgccaccg gcatcatcgg gccacgcgag
     3481 gaggccctgc ggttggcccg ttggatgatt ggccaactct ccctgcgcta ttcgcccgcc
     3541 gacctcagca tcggcttgct cgcgccggag tcggagcggg agtggtcgtg ggtcgccgaa
     3601 ctgccccact cacaggccga ggcggagcgc gacgcgggtc ggggtcgcgg ggtgatcctt
     3661 gtcgacggga ccccagcccc ggcaccgact gagaacgtgc acgtcctggc cgtggcggcc
     3721 gacgctgccg acctgccgag cgcctgcact cagatcgtcg agctgcgcgc cggcacctcg
     3781 agcctgacgt gggcgcagca gcctgtcact ggcctcgaac ccgatggggt cccgcgggcc
     3841 tgggccctcc tgctcgccca gcggctgggg ccgctgcgcg acgcggcagc gacccctcac
     3901 aagggcgtga ttccctctcg cacaaccttg ttggacgtcc tcgagattcc cgagcgagac
     3961 ggcatacccg acctcactgc ccgttggcgt gagagtcctc ggtcgacgct cactcccatc
     4021 ggggccaccg cctcggggcc gctgccgatc gacctcgtcc gcgacggccc ccacgcgttg
     4081 gtcggaggga cgacggggtc tggcaagtcg gagttgctcg tcagcctcgt ggccgggctc
     4141 gcggccgcga atcgccccga tgagctgagc ttcgtgcttg tcgactacaa ggggggtgcc
     4201 gccttcggcg cctgtcggga cctccctcac gtcgtcggcc tggtcaccga cctcgaccat
     4261 cagctgaccg agcgggcctt ggtcagcctc gatgctgagc tcaaacgacg cgagcgggtc
     4321 ttcggcgagc atggcgtcgc ggatctcgcc gcctaccaag gggtatgccg ccccggcgac
     4381 cccaccgtgg gacgtctggt catcgtcgtc gacgaattcc gcagtcttgc cgaggaattg
     4441 ccggagtttg tcaccggctt ggtgcgcgtg gcctcgcttg gccgatccct cggcgtgcac
     4501 ctcgtcatcg ctacgcagcg cccgggcgga gtcgtcaccg ccgacatgcg cgccaacctg
     4561 gggctgcgca tcgcgttgcg ggtccgcgac atcgtggact cgctcgacgt cgtcgaatcg
     4621 cccgtcgctg cctcgatcgc cgagagcacc ccagggcgag cgatcctcac cagcgcagcc
     4681 acccggctcg tcgaggtgca gaccgcgctc gcgaccggcc cgagccgccg agcggtcgag
     4741 ccgcccgtgc gggttgtgtc catcgatggg ctggccgttc cacagtcagt ccgggcgctt
     4801 gacggcccga cgcaggtgga cgacctcgtc gcagccagcc gtcgcgcggt gttggagctg
     4861 tcgattcccc tgcccgggtc gccctggttg cctccgctgt cgggtgactt gaccgtcgcg
     4921 agcctcgccg gaggttcagg atggccggtt ccgttggggc gcagagatgt tccccttcac
     4981 cagcggcaga gcgactggac ctgggacccc ctgcgtgaag gtcacctggg cattgtcggc
     5041 gcgccgcgga ccggccgcac cacggccctc ctcaccgtcg ccggacaact cgccgcccgc
     5101 ttctcccccg acgagattca cctgtatgcc gtgcacgccg gctccctggg ccatctcgac
     5161 gcactcccac atgtcggtgc ctcggtccgt tgcgacgagt tgccccggct ggcacaactc
     5221 ctgacgatcc tcaccgaccc cagcgccagc acccgcgcca gcagcagcac cagcagcagc
     5281 agcagcgaga cgatgcgcgt tctgctcgtg gacgactggg accgggtgtg cgaggagttg
     5341 gaccgggctc gagccggggt gctgcgcgac cggttgaccg cgctgctgcg tcgcggccca
     5401 cgggagcgcc tggccctctg cctcaccgga gggcgggcgt cactgtccgg gtcgatcggg
     5461 caactcgtgc cgcgtcggct cctgctcctg cccgccgagc cggtcgatct ggcgatggcc
     5521 gggctgtcgg ccagggccgt gccacaacac ccgacccctg ggcgtggcat tgacctggcc
     5581 gacggaagtg aggtccaggt cgccagcgtc gtggccgagc ccactgcggc gaggtgcacg
     5641 gcatacctgg aggagctggc ccggaatctg cctgcgcgcg agggtgactc gcccccgccc
     5701 accgtgccca ccctgcccgc agaggtgagc ctcagcgcac tccaacaccc gccgggtttg
     5761 ctggtcgtcg gcagcgacgg ccgcgaagca acgggtttcg accttgcc
//
LOCUS       DAOMZY010000234         5806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_13014_length_5806_cov_6.697096, whole genome shotgun sequence.
ACCESSION   DAOMZY010000234 DAOMZY010000000
VERSION     DAOMZY010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13014_length_5806_cov_6.697096"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..737
                     /locus_tag="PLK64_12365"
     CDS             171..737
                     /locus_tag="PLK64_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HPV80587.1"
                     /translation="MTSDDPGRATADLESASDFARVEDGFERLWTPHRMAYIEGVRPT
                     EEAGDGCPFCAAPLRSDIEGLIVARGELCFVVMNLFPYNPGHLLICPYRHVPLYVDLT
                     DEETTEFTAMTKTALAALTRTSAPMGFNIGMNQGRVAGAGVAAHLHQHVVPRWGGDSN
                     FLPIIGQTKALPLLLEDVRTRLAEVWPA"
     gene            836..1495
                     /locus_tag="PLK64_12370"
     CDS             836..1495
                     /locus_tag="PLK64_12370"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPV80588.1"
                     /translation="MLNRLLRASWTKVMTPIAKAFLAIGLTPDAVTIIGTLAVCVSAL
                     AFYPRGEFLTGTLLITLFIFSDNVDGVMARLSGRQGVWGAFLDSTLDRVGDAAIFGGL
                     VLWFAGPGANWLMAGLALACLILGMVVSYAKARAEGLGMTCNVGIAERADRLFAVLLA
                     TGLVGLGLPLWVLTVVLALLSVASFITVIQRMATVHTQAMAALRADGAGTAGDSGPTE
                     T"
     gene            1495..2451
                     /locus_tag="PLK64_12375"
     CDS             1495..2451
                     /locus_tag="PLK64_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HPV80589.1"
                     /translation="MGGLSGVLATARARGTVWGYRAGWSAVRRMPERAAYRVFDTVAD
                     VSLRKGSKGLARMRANYAKVRPELDDAALDALVREGMRSYTRYFCEAFRLPDCTHDDL
                     DRMCRAVNDAAVRANLDAGRSAVIFVPHMGNWDIAGAWSTGHLAPVTTVAERLEPEEV
                     FREFFEFRESLGMTILPLTGGPNPFNGLREAVERGDFVALVSDRDLTSNGVEVDFFGH
                     RARMAKGPALLALLTGAPLYAASIHFAPAAPGRGAAGRETVVTFSDEIPVPADGDTAA
                     KAQAMTQACAAYVEGPIREHTSDWHMMQRVFVDDLDPTRLGR"
     gene            2457..3638
                     /locus_tag="PLK64_12380"
     CDS             2457..3638
                     /locus_tag="PLK64_12380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPV80590.1"
                     /translation="MRIGIVCPYSFDIPGGVQFHVRDLAEILRSRGHTVSVLAPADEG
                     TELPDYVVSAGRAVPVSYNGSVARLNFGLMTNAKVTAWLDRGHFDLVHIHEPITPSIG
                     ALALWAADCPIVATFHTSNLRSRAMQVSYPFLRSSMEKILGRIAVSEDARRTLTTHFG
                     GDAVVIPNGVYVDRFASTLPRPQWQGQPGRPTIAFLGRMGEPRKGMPVLAAALPAVLA
                     QVPGLRLLVAGPGDADEVRAAMTPEVAAATEFLGAVDDAAKAALLRSVDVYIAPNTGG
                     ESFGIILIEAMSAGAAVLASDIPAFVRVLDGGTVGATFVNEDPDDLARQLVRLLRDDT
                     ERARLAAAGRARADLFDWSVVADAITLVYETVCEGAAAARAMPGHGVLGRLRRSGRSL
                     P"
     gene            3638..4213
                     /locus_tag="PLK64_12385"
     CDS             3638..4213
                     /locus_tag="PLK64_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80591.1"
                     /translation="MDGPNLWIVLALGLVAIGWYLTYTAARLDRLHARVQGTLAALDA
                     QLVRRAEATLELANARVLDAASALMLASAASDSLSAAEDEDTDADRQRFSNERETVES
                     RLSEALGLALTADTLASLPGAGGFGDDIVSRVRATGLRVQLARRFHNDAVTDVRRVRR
                     QWAVKWFRLAGHADMPQTVEFVDALPHGLRG"
     gene            4185..5297
                     /locus_tag="PLK64_12390"
     CDS             4185..5297
                     /locus_tag="PLK64_12390"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPV80592.1"
                     /translation="MRCRTGFEADAGMSAPSLPTPYLRLDVAKAQASIEAMAARARAL
                     DVGLRPHAKTHKSVRLGRLQLEAGAVGLTVATVGEAEVFVAGGCADIFIAYPLWVDAP
                     KARRLAAVLEWASVAVGVDSTASLDAMAAHGIRPEVVVEVDSGHHRSGVSPEAAGEIG
                     RYAADAGFVVRGVFTFPGHSYTPQTRASAAADEAIALAVAAESLAGQGIAVSVRSGGS
                     TPSAQFAVGRPGGSSGGGGLTELRPGVYVFGDAQQVELGTCAMDDVALTVVATVVSHA
                     GGRVILDAGSKALGADRAPWASGYGRLAEAPDARIVALSEHHATVEWSGGRLPQLGSR
                     VLVVPNHVCNTVNLADVYVIGDGPETWPVDARGCNS"
     gene            5342..>5806
                     /locus_tag="PLK64_12395"
     CDS             5342..>5806
                     /locus_tag="PLK64_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80593.1"
                     /translation="MENNSGVSSSTPSQEPSAAEARRALEALAADRRELADRMATPTW
                     YHPALAALVFILVAGYGFERLFLIALVLYSAGLVALVTAYRRITGVWSANLSRIGARR
                     YVAAFAVVLIVCLGLSNAARSGAVPWWWALVAGVVSGLSTLWVGPRLDAAVRS"
BASE COUNT          761 a         2033 c         2044 g          968 t
ORIGIN      
        1 accgggccgc cggcgcggtg tccttccggt tccgcgacgg gagccagcgc aacggcatac
       61 ctgttgccga ggctgccgcc gagatcctcg ccgcgatcgc ctcccggacc caggtttagg
      121 ttcggagaga cccctcaccg gtcgccgaag ccgccctcta gactcgggtc gtgacgtccg
      181 acgaccccgg cagggcgact gccgaccttg agagcgcgag cgattttgcg cgcgtcgagg
      241 acgggttcga gcggttgtgg acgccgcatc ggatggccta catcgagggc gtccggccca
      301 ccgaggaggc cggcgacggg tgcccgttct gcgcagcccc gctgcgcagc gacatcgaag
      361 ggctgatcgt cgctcgcggt gagttgtgct tcgtcgtcat gaacctcttc ccctacaacc
      421 cgggccacct gctcatctgc ccctatcggc acgtccccct ctacgtcgac ctcaccgacg
      481 aggagaccac cgagttcacg gcgatgacca agaccgccct cgccgcactc acccggacct
      541 cggctccgat gggcttcaac atcgggatga accagggccg ggtcgccggc gcgggggtgg
      601 ccgcccacct gcaccaacac gtcgtgccgc ggtggggagg cgactccaac ttcctgccga
      661 tcatcggcca gaccaaggcg ttgccgctgc tcctcgagga cgtgcggacg cgcctcgccg
      721 aggtctggcc ggcctgagcc gcacggtatg ccgcgcgccc accggtgcgc cgcggcaccg
      781 ggaccacacg gcatacccct aggctggtgg gcaccatgcc gaccccgagg ccctcatgct
      841 gaaccgactt ctgcgcgcgt cctggaccaa ggtcatgacg ccgatcgcca aggcatttct
      901 cgccatcggg ctgacgcccg acgcggtgac gatcatcggc actttggccg tgtgtgtctc
      961 ggcgctggcg ttctatcccc gcggtgagtt cctcaccggc acgttgctca tcacgctctt
     1021 catcttcagc gacaacgttg acggggtgat ggcccgcctg tccggtcggc aaggcgtctg
     1081 gggagcattc ctcgactcga ccctcgaccg ggtcggcgac gcggcgatct tcggtggcct
     1141 ggtgctgtgg ttcgccgggc ccggcgccaa ctggctgatg gccgggctcg cgctggcatg
     1201 cctcatcctc ggcatggtcg tgtcctacgc caaggcccgg gccgaaggcc tgggcatgac
     1261 ctgcaacgtg gggatcgccg agcgcgccga ccgattgttc gccgtcctgc tggccaccgg
     1321 cctggtcgga ttgggcctcc cgttgtgggt gctgaccgtc gtcctcgccc tcctgtccgt
     1381 cgccagtttc atcaccgtga tccaacggat ggccacggtg cacacccaag ccatggcggc
     1441 cctccgggcc gacggggcag gcacggccgg cgactccggg ccgacggaga cctgatgggt
     1501 ggtctgtcag gagtcctggc gaccgctcgg gcccgcggca ccgtctgggg ctaccgcgcc
     1561 ggttggagtg ccgtgcgtcg gatgccggag cgggcggcat accgggtctt cgacaccgtg
     1621 gccgacgtat cgctgcgcaa aggctccaag gggttggcgc ggatgcgcgc gaactacgcc
     1681 aaggtgcgcc ccgagctcga cgacgccgcg ctggatgccc tcgtgcgtga gggcatgcgg
     1741 tcctacacgc gctatttctg tgaggcgttc cggctgccgg actgcaccca cgacgacctc
     1801 gaccggatgt gccgcgcggt caacgacgct gccgtgcgtg cgaacctcga cgcggggcgc
     1861 tccgccgtga tcttcgtgcc gcacatgggc aactgggaca tcgccggggc gtggtcgacc
     1921 gggcacctgg ctcccgtcac gacggtcgcc gagcggttgg agcccgagga ggtcttccgg
     1981 gagttcttcg agttccgcga gtcgctcggc atgacgatcc tgcccctgac cggtgggccc
     2041 aacccgttca acgggctacg cgaagccgtc gagcgaggcg acttcgtggc cctggtctcc
     2101 gaccgtgacc tgacgagcaa cggcgtggag gtcgacttct tcggccaccg cgcccggatg
     2161 gccaagggcc ccgcgctcct cgcgctgctc accggcgccc cgctgtatgc cgcatcgatc
     2221 cacttcgcgc ccgcggcgcc ggggcgtggc gccgcaggcc gcgagaccgt cgtgaccttc
     2281 tccgacgaga tcccggtgcc cgccgacggc gacaccgcgg ccaaggccca ggcgatgacc
     2341 caggcgtgcg cggcatacgt cgaaggtccg atccgcgagc acacgtccga ctggcacatg
     2401 atgcagcggg ttttcgtcga cgacctcgac ccgacccgtc tggggcggtg accagggtgc
     2461 ggatcggcat cgtctgccct tactcgttcg acatccccgg cggggtgcag ttccacgtgc
     2521 gcgacctggc cgagatcctg cgctcacgcg gtcacaccgt gtcggtgctc gcccccgccg
     2581 acgagggcac cgaactaccc gactacgtcg tctcggccgg gcgcgccgtg ccggtgagct
     2641 acaacggctc ggtggcgcgg ctcaacttcg ggctgatgac caacgccaag gtcactgcgt
     2701 ggttggaccg tggtcacttc gatctggtcc acatccacga gccgatcacg ccgagcatcg
     2761 gtgcgctggc cctgtgggcc gccgactgcc cgatcgtggc gaccttccac acctcgaacc
     2821 tgcgctcgcg cgccatgcag gtgagctatc cgttcctgcg ctcgagcatg gagaagatcc
     2881 tcgggcggat cgccgtgtcg gaggatgccc gccgcaccct gacgacccat ttcggtggtg
     2941 acgccgtggt catccccaac ggggtgtacg tcgaccgatt cgcatcgacc ctgccccggc
     3001 cgcagtggca gggtcaacca ggtcggccga cgatcgcctt cctcggccgg atgggggagc
     3061 cccgcaaggg gatgcccgtg ctcgctgcgg cgctccctgc ggtgctcgcg caggtgcccg
     3121 ggttgcggtt gctggtggcc ggtcccggcg acgcggacga ggtgcgcgcg gcgatgaccc
     3181 ccgaggtcgc cgccgcgacc gagttcctgg gcgcggtcga cgacgccgcg aaggccgctc
     3241 tgctgcgctc ggtcgacgtc tacatcgcac cgaacaccgg tggggagagt ttcgggatca
     3301 tcctcatcga ggcgatgagc gccggggccg ccgtcctcgc cagcgacatc cccgccttcg
     3361 tgcgggtgct cgacggggga acggtggggg cgacgttcgt caacgaggac ccggacgatc
     3421 tggcccgcca actggtccgg ctgctgcgcg acgacaccga acgcgcccgc ctggcggctg
     3481 ccggccgagc ccgtgccgac ctgttcgact ggtcagtcgt ggccgacgcg atcacgctgg
     3541 tctacgaaac ggtgtgcgag ggggcggccg cggctcgagc catgcctggt catggggtcc
     3601 tcggtcgctt gcgccgcagc ggaaggagtc tgccgtgatg gatggtccca acctgtggat
     3661 cgtgctggcc ctcggcctgg tagcgatcgg gtggtacttg acctacaccg ccgcccggct
     3721 cgaccgcttg cacgcccggg tccagggcac gctggctgct ctggacgcgc aactcgtccg
     3781 gcgagccgag gccaccttgg agttggccaa cgcgcgagtc ctcgacgccg cgagcgctct
     3841 catgctggcc tccgccgcgt ccgactcgtt gtcggctgcc gaggacgagg acaccgacgc
     3901 cgaccggcag cggttcagca acgagcgcga gacggtcgag tcgcgcctgt ccgaggccct
     3961 cggcctggcc ctgaccgccg acacactggc gtcccttccc ggggcggggg gtttcggcga
     4021 cgacatcgtg tcgcgggtgc gagccaccgg gttgcgggtc cagttggccc gccgcttcca
     4081 caacgacgcg gtgaccgatg tccgccgggt ccgccgtcag tgggccgtca agtggttccg
     4141 gctggccggt cacgccgaca tgccgcagac cgtggagttc gttgatgcgc tgccgcacgg
     4201 gcttcgaggc tgacgccggg atgtcggctc cttccctgcc cacgccctac ctccgcctag
     4261 acgtggcgaa ggcccaggcg tcgatcgagg cgatggctgc tcgggcccga gcgctcgacg
     4321 ttgggctgcg accccatgcc aagacccaca agtcggtgcg gctgggtcgc ctgcaactgg
     4381 aggccggtgc ggtcgggctg actgttgcca cggtggggga ggccgaggtc ttcgtggccg
     4441 gcggctgcgc cgacatcttc atcgcctacc cgctctgggt cgacgcaccc aaggcccgac
     4501 ggttggcggc ggtgctggag tgggccagcg tggcggtcgg ggtcgactcg acggcgagcc
     4561 tggacgcgat ggccgcgcac ggcatacgcc cggaggtcgt ggtcgaggtt gactccggtc
     4621 accaccgcag cggcgtctcg cccgaggcgg cgggcgagat cggccggtat gccgcggacg
     4681 ccggtttcgt ggtccgcggc gtgttcacct tccctgggca ctcctacacg ccgcagacgc
     4741 gggcatcagc ggccgccgac gaggcgatcg cgctggccgt ggccgccgaa tcccttgcgg
     4801 gccaagggat tgcggtgtcg gttcgaagcg gggggtcgac gccatcggcg cagttcgctg
     4861 tggggcgccc ggggggaagc agcggcgggg gagggctcac tgagttgcgc cccggcgtct
     4921 atgtctttgg tgacgcccaa caggtcgagt tgggcacctg cgcgatggac gacgtcgcgc
     4981 tcaccgttgt cgcgacggtc gtctcgcacg ccggcggccg ggtgatcctc gatgcgggca
     5041 gcaaggcgct cggtgccgat cgggcgccgt gggccagcgg ttatggccgg ttggcggagg
     5101 ctcccgacgc tcgaatcgtc gccttgtccg agcaccacgc caccgtcgag tggtccggcg
     5161 ggcgactgcc gcagttgggc tcgcgggtgc tggtcgtgcc caaccacgtg tgcaacacgg
     5221 tgaaccttgc cgacgtctac gtcatcggtg acggcccgga gacttggccg gtcgacgcgc
     5281 ggggctgcaa ctcgtaaccc tggggctttc cgttccggaa accttgtgct agttttccgg
     5341 tatggaaaac aactcgggtg tgtcgtcatc gacaccgtca caggagccgt ccgcagcgga
     5401 agcccgccgg gccttggagg cgctcgccgc cgatcgtcgg gagttggccg accgcatggc
     5461 cacgcccacc tggtaccacc cggccctggc tgcgctggtc ttcatcctcg tggccggcta
     5521 tggcttcgag cggttgttcc tgatcgcttt ggtgctctat tcggcgggcc tcgtcgctct
     5581 cgtgacggca taccggcgga tcaccggtgt gtggtcagcc aacttgagtc gcatcggtgc
     5641 ccgacgctac gtggccgcct tcgccgtcgt cctcatcgtc tgccttggtc tctcgaacgc
     5701 tgcgcgctcg ggtgccgtcc cgtggtggtg ggccctggtg gcgggcgtgg tgagcggcct
     5761 gagcaccctc tgggtggggc cgcggctcga tgcggccgtg cgctcc
//
LOCUS       DAOMZY010000235         5806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_13015_length_5806_cov_6.128847, whole genome shotgun sequence.
ACCESSION   DAOMZY010000235 DAOMZY010000000
VERSION     DAOMZY010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13015_length_5806_cov_6.128847"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PLK64_12400"
     CDS             <1..660
                     /locus_tag="PLK64_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HPV80594.1"
                     /translation="APDVVEGVGRIAAKAHARGVHVAIHTHVNHVNSVTPLVAETARA
                     LMDAGLFNIRNQGVLLRGINDTPKDLLDLSFALQDNASITPYYFYMCDMIPFSEHWRL
                     SLAEAQHLQHAIMGYLPGFATPRIVCDVPFVGKRWVHQVEEYDREHGISYWRKNYRTS
                     IEAADMDALSREYVYYDPIYTLPDEGQKWWARETNAAAAHAAAFDAAALSRLASAQSL
                     T"
     gene            complement(626..2113)
                     /locus_tag="PLK64_12405"
     CDS             complement(626..2113)
                     /locus_tag="PLK64_12405"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPV80595.1"
                     /translation="MSHQRVLIVGSGFSGIAMAARLAQAGVDDFLILEKADDVGGTWR
                     DNRYPGCACDVPSRLYSLSFAPKADWSRDYAPAGEIWDYLRDCVERFGLRSRIVFGAE
                     VTEATWKRGRWEVLAADGREWTAGALVLGVGGLHVPRRPDLPGLGSFAGRVLHTADWP
                     EDDHLSGVRVGVVGTGASAVQLVPSLADRVGALEVFQRTPSWILPRDDRPWSRARQQS
                     YARMPLLGRLARWRTYVRLETRVLGFGRLDRARRLVQEQALARLAEAVPDPATREALT
                     PHYALGCKRVLLSDDYWPTFARPDVALVTAPIDRVEPWGVVTEDGEHHELDALVFATG
                     FDVRGSFDRVRITGLRGRTLADEWASSRSTHLGIEVAGFPELYLLLGPNTALGHNSVL
                     VMIEAAIEHILKALHRADEEGPHVVTRSAQARFGRWVRQRTRHTVWGSGCQSWYLDER
                     GRNVAIWPASSIRYRVATRRLRARSYERVTEDGPVRSMTGPTRVD"
     gene            2240..2725
                     /gene="ybaK"
                     /locus_tag="PLK64_12410"
     CDS             2240..2725
                     /gene="ybaK"
                     /locus_tag="PLK64_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HPV80596.1"
                     /translation="MAKASGTPATTALTRAKVAFTVHTYEHDPAAPSYGLEAAAALGV
                     DPARVFKTLLVEADGRLGVGIVAADRLLDLKAIASALGAKKAVMADPAVAERSTGYVV
                     GGISPIGQKRALPTVLDADATAYSTILVSGGRRGLDVELSPADLLAMTAGRTAPIARD
                     R"
     gene            2806..4050
                     /locus_tag="PLK64_12415"
     CDS             2806..4050
                     /locus_tag="PLK64_12415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HPV80597.1"
                     /translation="MIVRRASDRGIVVLLAALTMLGPFTVDTIFPGFPAVQREFTVDA
                     VATQQIVSVYLFTFAVMSLLHGPLSDALGRRPVIVGGLAMFTVTSVLCALAPSMTWLL
                     VGRALQGVFAGGGMIVGRTVVRDVFSGHPAQRAMSQMSMIFGVAPALAPIVGGWLLGW
                     SSWRSIFWFLAIFAVVVLAAVMLLLPETHPPTRRTPLSTRALVSAVTDSSKDPSVRRL
                     LLVSSFNFAALFTYISAAPAFIVDHLHLGPGDFGWLFVPVVTMMIVGSWLTGRLAGRI
                     SGPRFLTLGFGVAVLGGVVQLALDVVGLRTLPWVLVGPVTTGLGIALVFPIVTLVLLE
                     VRPRHRGTLSSMQSFSNTMLNAIVAGALVPLVSASLAGLTGAAVTFSIAGWLVWARHH
                     RMAHPDVSAPPDPESYEPTDRM"
     gene            complement(4127..4588)
                     /locus_tag="PLK64_12420"
     CDS             complement(4127..4588)
                     /locus_tag="PLK64_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HPV80598.1"
                     /translation="MEKLDRKLVQLLAADGRMSYTDLGKATGLSTSAVHQRVRRLEER
                     GVVQGYQAVIDPAQVGLPLTAYISLSPLDPAAPDDIPDRIRGFSEIESCHSVAGDFNY
                     ITKVRVATPLDLEDLLTRLRGAAHVQTRTTVVLSTAWEGRPVTGRDTPPKA"
     gene            4713..5732
                     /locus_tag="PLK64_12425"
     CDS             4713..5732
                     /locus_tag="PLK64_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HPV80599.1"
                     /translation="MDQAGRLLLLDSASLYFRAFYGVPDQRTDPSEPPTNAIRGFLDM
                     VATLVDRHRPTHLAACWDNDWRPQFRVQAIPSYKTHRVAAGADPSAPGGVAGAGGAAA
                     SGASGASGDGSSGLTSEEAPDDLAPQVPLIAAALAALGIARVGVDGFEADDVIGALAH
                     RHRGALAVDIVTGDRDLFQLVDDALGVRVLYTSRGGVRDAEIVDQAVLQERYAVPTGD
                     AYADLAVLRGDASDGLPGVAGIGEKTAAALLATYGSLAGIRAAVDGGDPRIKGAQRTR
                     LEAGAAYLDVAPGVVRVAVDAPLPDVDLRLPTQVADPVLMSRLAVDYGLTGAFNRVLS
                     TLGVG"
BASE COUNT          808 a         2066 c         2007 g          925 t
ORIGIN      
        1 gcccccgacg tcgtcgaggg cgtcggtcgg atcgccgcca aggcgcacgc gcgtggggtc
       61 catgtcgcga tccacaccca cgtcaaccac gtcaactcgg tcaccccgtt ggtggccgag
      121 acggctcggg cgctgatgga tgcgggcctg ttcaacatcc gcaaccaggg cgtgctgttg
      181 cgcgggatca acgacacccc gaaggatctg ctcgacctgt cgttcgcgct gcaggacaac
      241 gcgtcgatca cgccgtacta cttctacatg tgcgacatga tcccgttcag cgagcactgg
      301 aggttgtcgc tggccgaggc tcagcacctg cagcacgcga tcatgggcta cctgcctggg
      361 ttcgccacgc cgcgcatcgt ctgcgacgtg cctttcgtcg gtaagcgctg ggtgcaccag
      421 gtcgaggagt acgaccggga gcacgggatc tcctactggc gcaagaacta ccgcacgtcc
      481 atcgaggccg ccgacatgga tgcgctctcg cgcgagtacg tctactacga cccgatctac
      541 accctccccg atgagggtca gaagtggtgg gcccgcgaga ccaacgccgc cgcggcgcac
      601 gctgccgcgt tcgacgccgc tgccctcagt cgactcgcgt cggcccagtc attgacctga
      661 ccggcccgtc ctcggtgacg cgctcgtagg agcgcgcccg aaggcgtcgg gtcgccaccc
      721 ggtagcggat cgaggacgcc ggccagatgg cgacgttgcg cccgcgttcg tcgaggtacc
      781 agctctggca ccctgagccc cacaccgtgt ggcgcgtccg ttgcctgacc cagcgcccga
      841 accgggcctg ggcgctgcgg gtgacgacgt gtgggccctc ctcgtcggct cgatgcaagg
      901 ccttcaggat gtgctcgatc gcggcctcga tcatgacgag caccgagttg tggccgagcg
      961 ccgtgttcgg cccgagcagc agatagagct cggggaaacc ggcgacctcg atgcccaggt
     1021 gcgtcgagcg gctggacgcc cactcgtcgg cgagggtgcg tccccgaagg ccagtgatgc
     1081 ggacccgatc gaaactcccc cgcacgtcga atccggtcgc gaacaccagg gcgtcgagct
     1141 cgtggtgctc gccgtcttcg gtgacgaccc cccaaggctc gacccggtcg atcggtgcgg
     1201 tcaccaacgc gacgtccggg cgggcgaagg tcggccagta gtcgtcggac agcaggaccc
     1261 gtttgcaccc caacgcatag tgcggggtca gtgcttcgcg ggtcgccgga tcggggaccg
     1321 cctctgccaa gcgtgccagg gcctgctcct gcaccaaacg acgggcgcgg tccagtcgcc
     1381 cgaagcccag cacccgcgtc tccagacgca cataggtgcg ccaccgtgcc agccgcccga
     1441 gcagcggcat ccgggcgtag ctctgctggc gggcgcggga ccacgggcgg tcatcccgcg
     1501 gcaagatcca cgagggcgtc cgctggaaaa cctccaacgc gccgacccga tcggccaggc
     1561 tcggcacgag ttggacggcc gaggcgccgg tgccgaccac gccgacccga actccggaga
     1621 ggtggtcgtc ctcgggccaa tcggcggtgt gcagcacccg accggcgaac gatccgagcc
     1681 caggcagatc cggccgacgc gggacgtgca gcccgccgac accgagcacg agggcccccg
     1741 cggtccattc ccggccgtcg gccgccagca cctcccaacg gccccgcttc caggtcgcct
     1801 cagtcacctc ggcgccgaag acgatccgtg agcgcagtcc gaagcgctcg acacagtcgc
     1861 gcaggtagtc ccagatctcg ccggccggcg cgtagtcgcg gctccagtcg gccttcgggg
     1921 cgaaactcag tgaatagagg cggctgggca cgtcgcaggc acacccgggg tagcggttgt
     1981 cgcgccaggt ccccccgaca tcgtccgcct tctccagaat gaggaagtca tcgacgccgg
     2041 cctgcgccaa ccgggcagcc atggcgatac cggaaaagcc tgaccccacg atgagcaccc
     2101 gctggtgaga cacagccgca gcgtatgccg cctcggcccc ggacgtcaca ctccgggagc
     2161 agtccggcga cagcggtcgg gctggatacg gcggtgccgc ggcttcggcg atgggcggga
     2221 gacggcatac tcgcgtctca tggcgaaggc gtccggaacc ccggcgacca ccgcgctcac
     2281 tcgggcgaag gtggcgttca ccgtccacac ctacgagcac gatccggcgg cgccgtccta
     2341 tggcctggag gctgctgccg cgctcggcgt cgaccccgcg cgagtcttca agaccctgct
     2401 cgtcgaggcc gacgggcggc tcggcgtcgg catcgtcgcc gccgaccggt tgctcgatct
     2461 caaggcgatc gcgtcggccc tcggcgccaa gaaggccgtc atggccgacc cggctgtcgc
     2521 cgagcgctcg accggttacg tcgtgggcgg tatctcgccc atcgggcaaa agcgggcgtt
     2581 gccaacggta ttggatgccg acgcgacggc atactcgacg atcctcgtgt ccggcggacg
     2641 ccgtgggctc gacgtggaac tctcccccgc cgacctgctc gccatgaccg cgggccgcac
     2701 cgcacccatc gcccgagacc gctgagccgc ccaacgcgcg aggtcccgtg ggtcatcggg
     2761 tggaccgctc cgcgccgctg tggccacgcg gcgtagcgtg cttgagtgat cgtccgccga
     2821 gcatctgacc gaggaatcgt cgtcctgctg gcggccctga ccatgctggg gccgttcacg
     2881 gtcgacacga tcttccctgg cttcccggcg gtccaacgcg agttcaccgt cgatgctgtc
     2941 gccacccagc agatcgtctc ggtctacctc ttcaccttcg ccgtgatgag cctgttgcac
     3001 gggccgctgt ccgacgccct gggccgacgc ccagtcatcg tcggcgggct ggcgatgttc
     3061 acggtgacct cggtgctctg cgcgctcgcc ccctcgatga cctggctgct cgtcggccgg
     3121 gcccttcagg gggtcttcgc cggtggcggc atgattgtgg ggcgcaccgt ggtccgggac
     3181 gtgttctctg gccacccggc ccaacgcgcc atgtcgcaga tgtcgatgat cttcggggtg
     3241 gccccagctc tggcccccat cgtcgggggc tggctgcttg gctggtcgag ctggcgcagc
     3301 atcttctggt tcctcgcgat cttcgcggtg gttgtcctcg ccgcagtgat gctgctgctt
     3361 cccgagaccc acccgcccac tcgtcggacc ccgctctcga cgcgggcgct cgtgagcgcg
     3421 gtcaccgact ccagcaagga ccccagcgtg cggcgcctgc tgcttgtgtc gtccttcaac
     3481 ttcgcggcgc tgttcaccta catctccgcg gcccccgcct tcatcgtcga tcacctccat
     3541 ctggggccag gcgacttcgg ctggttgttc gtgcccgtgg tgacgatgat gatcgttgga
     3601 tcctggctga ccgggcggct ggccgggcgg atcagcgggc cccgcttcct caccctcggg
     3661 ttcggggttg ccgtgctggg cggtgtggtc cagttggcac tggacgtggt cggtctgcgg
     3721 actctcccct gggtgctggt cggtcccgtg accacgggtt tggggatcgc gctcgtcttc
     3781 cccatcgtta ccctcgtgct gcttgaggtg cggccgcgcc atcgagggac gctgtcatcg
     3841 atgcagtcgt tcagcaacac gatgctcaac gccatcgtcg ccggagcact cgtgcccctc
     3901 gtcagcgcca gccttgccgg gctgaccggc gcggcggtga ccttcagcat cgccggatgg
     3961 ctggtgtggg cacggcatca ccggatggca caccctgacg tgtccgcgcc gccggaccca
     4021 gagtcctatg agccgaccga ccggatgtag aggccgcgcg gacgtcggct gagcggcggg
     4081 ccgctgggcg gcatacctcc gggtgctagg tgcgagagcg ctgcggtcag gccttcggcg
     4141 gggtgtcgcg tccggtgacc gggcggcctt cccacgcggt ggagagcacg acggtcgtgc
     4201 gcgtctggac gtgagcggca ccgcgcaggc gggttagcag gtcctccagg tcaaggggcg
     4261 tggccaccct gaccttggtg atgtagttga agtcgccggc caccgagtgg cacgactcga
     4321 tctcggagaa gccgcggatc cggtcgggga tgtcgtcggg cgcggcgggg tcgagaggcg
     4381 acagcgagat atatgccgtg agcggcagcc ccacctgggc cgggtcgatg acggcttggt
     4441 aaccctgcac cacgccacgc tcttccagac gccggacccg ctgatgcacg gcggaggtcg
     4501 acaggcccgt cgccttgccg aggtccgtgt agctcatccg gccgtccgcc gcgagcagct
     4561 gcacgagctt ccggtcgagt ttctccatgg gggcagccta cgcagtcaac gggaccggcc
     4621 gctggcccgt ttggggggtg ggacgtcggg ccttcgcacg gtcgccgaac gcggctgtcg
     4681 cacccctgcg gaccgaacta gggtgaggcc ccatggacca ggctggcaga ctcctcctcc
     4741 ttgactcggc gagcctgtat ttccgggcct tctatggcgt gcccgatcag cgcacggacc
     4801 cgtccgagcc cccgacgaat gcgatccgcg gcttcctcga catggtggcc accctggtcg
     4861 accggcaccg gccgacccat ctggccgcat gttgggacaa cgactggcgg ccgcagttcc
     4921 gggtgcaggc gatcccgtcc tacaagaccc atcgggtcgc tgcgggtgcc gacccgagcg
     4981 ctcccggcgg cgtggccgga gccggcgggg ccgcggcgtc gggcgcgtca ggcgcgtcgg
     5041 gcgatggctc gtcggggttg acctccgaag aggctcccga cgacctcgcg ccccaggtcc
     5101 cgctcatcgc tgccgccctg gccgccctgg ggatcgcgcg cgtgggggtc gacgggtttg
     5161 aggccgacga cgtcatcggg gcgctggccc accgccaccg gggcgctctc gcggtcgaca
     5221 tcgtcaccgg cgaccgtgac ctcttccaac tggtggacga cgcgctgggt gtgcgggtcc
     5281 tctacacctc ccgcggcggg gtgcgcgacg cggagatcgt ggaccaggcc gtgctgcagg
     5341 agcggtatgc cgtgcccacc ggcgacgcct atgccgacct cgccgtcctg cgcggcgatg
     5401 cgtccgacgg gctccccgga gtggccggga tcggcgagaa gacagcagcg gccctgctgg
     5461 cgacctacgg gtcgctcgcg ggcatccggg ctgccgtcga tggcggcgac ccccggatca
     5521 agggagccca gcgcacccga ctggaggccg gggcggcata cctcgatgtc gctcctggcg
     5581 tcgtgcgggt ggcggtggac gcgccgcttc ccgacgtcga cctgcgcctg cccacccagg
     5641 tggccgaccc ggtgctcatg tcgcgcctgg ccgtcgacta cggcctcacc ggagcgttca
     5701 accgggtgct gtccaccctc ggcgtcgggt gatcgagcgg ggcgagcggg cgctgccctt
     5761 gccggagcgt tagccacctt cggatgtggc caacgctccg aggatc
//
LOCUS       DAOMZY010000236         5790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_13061_length_5790_cov_4.825283, whole genome shotgun sequence.
ACCESSION   DAOMZY010000236 DAOMZY010000000
VERSION     DAOMZY010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13061_length_5790_cov_4.825283"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..5241)
                     /locus_tag="PLK64_12430"
     CDS             complement(184..5241)
                     /locus_tag="PLK64_12430"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPV80600.1"
                     /translation="MTISTKDINAFLLQAARDGVIDRGMGAAILKQVNAKRRRTDIAI
                     VGLECAFSQATTKEQFWANLVQGRTSIRTMPRARANDVEDSLGITRSDEHAHYSAMGY
                     LPQIAEFDASFFRISPKEALVIDPKQRLFLQVLYRAFEDAGYGGAALTGTKTGVYVGV
                     DHLNDTEFPYKALVKEPGMLAQTGAMPGLMAGRPSYLFDLSGPAVVFDTACSSSLVAL
                     HAACEGLYAGECDMAVAGGVNVSIYTRGGTNFQSVESGGGAVRTFDRRASGTNWGEGV
                     AAMVLKRLDDAISDGDHVYAVVKAVALNNDGATNGVTAPSSEAQERVIVQAWKDADID
                     PGQLSYLEAHGTGTTVGDRIEMRALTNAFERFTTKRQFCGLGTAKPNIGHAVAASGMA
                     GLFKVVMAMEHGTLPPSVNFEEPNPEIGFANSPMFMVDSPLPWPKDTARLAGVSSFGF
                     SGTNSHAVLASPPPQTSPSVGDGLEVLTLSTHARTLWPALLDAYLEHLQAHPDSSLAD
                     LCYTANTGRRHSKWRVALTVATVEDLVAGLTDLRAQVAAGADLGAEPRTEGRNEAFAE
                     MSAAVDRYRQSGRTDRATLERVAELYRTGIDTDWRTFYSGSPRKRLALPGSPLLPTRF
                     WAEHSDQPTPTGAASLWQQTGRAQAARPVTSTTDWALADALVAPTPELLAGSWLVLRD
                     AGPTGDRVTAYLNDAGARVVEVTANDRGEYVGGDDRYTLDPDRPQDLDLLVEDLKRTE
                     AVPTHVVYLWSLDAPDGDLRNRPFDTLVAMMRAVSGLLGGQTSALTIVTSGAFGFDGQ
                     PSPSALVAGAAIVAAHEYPKLAIRHVDTDLDAEAATLAARILAEAIGGSEPSPIAYRG
                     DARLTRRHRSVEIADGSGGCALRSGVYLLTGGLGGIGEALAEHLLSTHHASVVLTGRT
                     ALPNETEWPTVSVATHGTEVAARIATMVALRRHEVPVEYHAVDLTDLTAMSALIDDVE
                     VRLGPITGVFHLAGVASGRMIHFLTPGTSAPVLEPKVDGTLVLDQVFADRDVEFVMLF
                     SSVLAILGPTGSSEYAAACSFEDAYARAARIRYPQRRTLSVNWDRWAHVGMAARSEAQ
                     APQGTAAARMMADGLTLEDGIGFIDPLLRSALVNVAVTSQSIDEMRAVLEGSDDAVLG
                     EGHGEPKFDRPELSADFVAPRTDVEANMAAMWEGVFSIRGIGVNDSFYELGGDSLHAI
                     SLTSVLNQAYPVEVTDLYAYPTIAELAAYVGGMGGSADLREQLQEVGRWFIDGGWRRE
                     GNAHIAERRDAYAAHVPLASTLGAALDTDAVTYREVLVLGATGFVGVHLLHEVLQETT
                     ASVHVLVRPGGAGPAERLRAAYTRFFSDDDFRWHQPRIHLLVGDAAQPHFGLAEQDYE
                     GLAGRIDCVLNSAGKVDHVGRRDDFVGPNVSAVRQILEFAATGIAKDIHHVSTRGVMG
                     LMPGNDEIFTEFDDNLGQQPVNAYTDTKLEAELLLHEARRAGQVVTIYRLNAITGDSG
                     DGTFQENIDQNAFYLQMRALYRFACLPQGDLRLWDFTPVDAACRRLVALMGHRSLHGQ
                     TYHLLRSTKVGVPEFAAAFAAIGQEKPLVTVAEFMDYLAENYDGSPITPFVREFVLHS
                     KLVEIPQLAGYDIVTDWTRAVLEHLGMPFDEDVDRDVLTTVRYARDSGFLDAVARPPA
                     AIANPPAPDFLTPAGVL"
     gene            complement(5238..>5790)
                     /locus_tag="PLK64_12435"
     CDS             complement(5238..>5790)
                     /locus_tag="PLK64_12435"
                     /inference="COORDINATES: protein motif:HMM:NF013166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HPV80601.1"
                     /translation="AGPFAVFCHSVGTLLAFEVLHSLQEHEGIEPVHVFFSGRHTPDV
                     PSKTSWHQAPDSVFLSMIRGFGGTSQQVLDTPELVELFTPILKSDYKVAETYEYAAPA
                     ELLHCPVTAVAGTRDPVVSAEQLRRWGEFTDGPFDVHQLPGGHFYWFDDVAPLCSILM
                     RGLARPSSPDRSTSARDGQVHQL"
BASE COUNT          940 a         1893 c         2038 g          919 t
ORIGIN      
        1 gctgcggagc gagcgcggca acggcagcgc gcagcagcgg ggcggtcgcc tcggagggat
       61 tcggcacgac cgacaagcct agccggtatg ccgtctggtc accttcggac gcactcggct
      121 cgggctgacc tgcccgattt cctcacaccc accgatgttt tcgcgtccct agacgctagg
      181 gagtcacaga acgccggcgg gtgtgaggaa gtcgggagca ggcgggttgg cgatcgcagc
      241 cggtggtctg gccaccgcat cgaggaagcc ggagtctcgc gcataccgga ccgtggtgag
      301 cacgtccctg tcgacatcct cgtcgaacgg cataccgagg tgttccagca ccgcgcgcgt
      361 ccagtcggtg acgatgtcgt agccggccaa ttgggggatc tcgaccagct tggaatgcag
      421 gacgaactct cggacgaacg gagtgatcgg ggatccgtcg tagttctccg ccaggtagtc
      481 catgaactcc gccacggtga ccaaaggctt ctcctgcccg atggccgcaa acgctgcggc
      541 gaactcgggg acaccgacct tcgtcgatcg cagcagatgg taggtctgcc catgcagcga
      601 tcgatgaccc atgagtgcga cgagacgtcg acaggccgca tcgaccgggg tgaagtccca
      661 cagccgaagg tcgccttgcg ggaggcaggc gaaccgatag agcgcccgca tctgcaggta
      721 gaacgcgttc tggtcgatgt tctcctggaa cgtgccgtcg cccgagtcgc cggtgatcgc
      781 gttgagccga tagatcgtca cgacctgacc ggcccgacgc gcctcgtgga gaaggagctc
      841 ggcctccaac ttggtgtcgg tgtaggcgtt caccggttgc tggccgaggt tgtcgtcgaa
      901 ctccgtgaag atctcgtcgt tccccggcat cagccccatg acgccgcggg tcgacacgtg
      961 gtggatgtcc ttggcgatcc ccgtggcagc gaactcgagg atctggcgca ccgcgctcac
     1021 gttgggcccc acgaagtcgt cccgccgccc gacatgatcg accttgccgg ccgagttgag
     1081 cacgcagtcg atccgcccgg ccagaccctc gtagtcctgc tccgccagac cgaagtgcgg
     1141 ctgggccgcg tcgcccacga ggaggtggat gcggggctgg tgccaccgga agtcgtcgtc
     1201 ggagaagaac cgggtgtatg ccgcgcgcag ccgctcggcc ggaccggcgc caccgggtcg
     1261 caccagcaca tgcacactcg cggtcgtctc ctggagcacc tcgtgcaaga ggtggacgcc
     1321 gacaaacccc gtcgcaccca ggacgaggac ctcgcggtag gtcaccgcat cggtgtccag
     1381 ggcggcgccc agggtcgacg ccaggggcac gtgggcggca tacgcgtccc gtcgttcggc
     1441 gatgtgcgcg ttaccctcgc ggcgccatcc gccgtcgatg aaccagcggc cgacctcctg
     1501 gagttgctcg cgcaggtcgg cactgccgcc catccccccg acgtatgccg ccaactcggc
     1561 gatcgtcggg taggcgtaga ggtcggtcac ctccaccgga taggcctggt tgagaaccga
     1621 cgtcaggctg atcgcgtgca gggagtcgcc gcccaactcg tagaagctgt cgttgacccc
     1681 gatgccgcgg atcgagaaga cgccctccca catcgcggcc atgttggcct cgacatcggt
     1741 acgcggcgcg acgaagtcgg cggacagctc aggccgatcg aacttcggct cgccgtggcc
     1801 ttcgccaagg accgcgtcgt cgcttccctc gagaacggcg cgcatctcat cgatcgactg
     1861 gctcgtgacg gccacgttga ccagagcgct gcgcaggagg ggatcgatga acccgatgcc
     1921 gtcctccagg gtcaggccgt cggccatcat ccgcgccgcg gccgtccctt gcggggcctg
     1981 cgcctcgctg cgcgcggcca ttcccacgtg tgcccaacgg tcccagttga cgctgagggt
     2041 gcggcgctgc ggataccgga tccgggccgc ccgggcatag gcgtcctcga aggagcacgc
     2101 ggcggcatac tcgctcgaac cggtgggacc gaggatggcc aggaccgacg agaagagcat
     2161 gacgaactcg acgtcccggt cggcgaagac ctggtccaac accagcgtgc catccacctt
     2221 gggctcgagg accggggcac tcgtcccggg cgtaaggaag tggatcatgc ggccgctggc
     2281 gacgccggcc aggtggaaga caccggtgat cgggccaagt cgcacctcga cgtcgtcgat
     2341 gagcgccgac atcgccgtga ggtcggtgag gtcgaccgcg tgatactcca cggggacctc
     2401 gtgtcggcgc aacgcgacca tcgtggcgat ccgggccgcg acctcagtgc cgtgggttgc
     2461 cacgctcacc gtcggccact cggtctcatt gggcagcgcg gtgcgcccgg tgaggacgac
     2521 gctcgcgtgg tgagtgctca gaaggtgctc ggcgagcgcc tcgccgatgc cccccaaccc
     2581 tccggtgagc agatagaccc cggatcgcag ggcgcacccc ccagagccgt cggcgatctc
     2641 caccgaacgg tgccggcggg tgagccgagc gtctccgcga taggcgatcg gcgagggctc
     2701 ggacccaccg atggcctcag cgaggatgcg cgccgccaac gtcgcggcct ccgcgtcgag
     2761 gtcggtgtcg acgtgccgga tggcaagctt cgggtattcg tgggcggcca cgatggccgc
     2821 cccggcgacc aacgcgctgg gtgatggctg gccatcgaac ccgaacgctc ccgaggtgac
     2881 gatcgtcagg gccgacgtct gcccgccgag cagcccggaa acggcccgca tcatcgccac
     2941 cagcgtgtcg aacggacggt tgcgtaggtc gccgtcgggt gcgtcgaggc tccacagata
     3001 gacgacgtgg gtcggcacgg cctcggtccg cttgaggtcc tccacgagga ggtcgaggtc
     3061 ctgcggtcga tcggggtcga gggtgtagcg gtcgtccccg cccacatact ctccgcggtc
     3121 gttggccgtc acctccacca ctcgggcacc ggcgtcgttg aggtatgccg tgacgcgatc
     3181 gcctgtggga ccggcgtcac gcaggaccag ccagctcccc gcgagcagtt ccggcgttgg
     3241 tgccaccagg gcgtctgcga gcgcccaatc cgtggtcgac gtgaccggcc gggccgcttg
     3301 agcacgcccc gtctgttgcc agagggacgc tgccccggtg ggggtcggct gatcgctgtg
     3361 ttccgcccag aaccgggtcg gcaacaacgg cgagcccggc agggcaagcc gcttgcgggg
     3421 actgcccgag tagaaggttc gccagtccgt gtcgataccg gtccggtaga gctcggcgac
     3481 gcgttcgagg gttgctcgat cggtgcgtcc ggactgtcga tagcggtcca cggcggcgga
     3541 catctcggcg aaggcctcgt tgcgaccctc agtcctgggc tcggcgccca ggtccgcccc
     3601 ggcggccacc tgggcacgca ggtcggtgag cccagccacg aggtcttcaa cggtggcgac
     3661 cgtcaaggca actcgccact tgctgtggcg ccgtccggtg ttcgcggtgt agcacaggtc
     3721 agctagcgag ctgtccggat gggcctgcag gtgctcgaga taggcgtcca gcagggccgg
     3781 ccacagggtc cgagcatgcg tcgagagggt caacacctca agcccgtcgc cgactgacgg
     3841 ggaggtctgc ggcggcggcg acgcgaggac ggcgtggctg ttcgtgccgc tgaagccgaa
     3901 ggagctcacc ccggcgagcc gagccgtgtc cttcggccat gggagggggc tgtcgaccat
     3961 gaacatcggt gagttcgcga agccgatctc ggggttcggc tcctcgaagt tgacactcgg
     4021 cggcagggtt ccgtgctcca tcgccatgac gactttgaag aggccggcca tcccggatgc
     4081 cgcgaccgcg tggccgatgt tgggcttcgc cgtgccgagg ccgcagaact ggcgtttggt
     4141 cgtgaaacgt tcgaaggcgt tcgtcagggc gcgcatctcg atccgatcac cgaccgtggt
     4201 tccggtgccg tgggcctcga gataggacaa ctgcccaggg tcgatgtcgg cgtccttcca
     4261 agcttggacg atgacccgct cctgggcttc cgaggacggc gcggtgacgc cgttcgtggc
     4321 cccgtcgttg ttgagggcga ccgccttgac caccgcgtag acgtgatccc cgtcggagat
     4381 ggcgtcatcc aggcgcttga gcaccatggc ggccaccccc tcgccccagt tcgtgccgct
     4441 ggctcggcga tcgaaggtcc gcacggcacc gccgccggac tcgacgctct ggaagttggt
     4501 cccccctcgg gtatagatgc tgacgttgac gccgccggcg accgccatgt cgcactcacc
     4561 ggcatacagt ccctcgcaag cggcgtgcag cgcgacgagc gacgaggagc acgccgtgtc
     4621 gaagacgacc gccggcccgg agaggtcgaa gaggtaggag ggtcgccccg ccatgaggcc
     4681 tggcatggca cccgtctggg ccagcatccc cggctccttg acgagtgcct tgtagggaaa
     4741 ctcggtgtcg ttgagatggt cgacgccgac atagaccccg gtcttggtgc cggtgagggc
     4801 cgcccctccg tagccggcgt cctcgaaggc gcggtagagc acctgaagga agagccgctg
     4861 cttcgggtcg atgacgaggg cttccttggg cgagatgcgg aagaacgacg cgtcgaactc
     4921 ggcgatctgg ggcaggtacc ccatcgcgga gtagtgggcg tgctcgtccg accgggtgat
     4981 cccgagcgag tcctcgacgt cgttggcgcg ggcgcggggc atggtccgga tgctcgtccg
     5041 tccttggacg aggttggccc agaactgctc cttggtcgtg gcctgggaga aggcgcactc
     5101 gagaccgacg atggcgatgt cggtgcgacg ccgcttggca ttcacctgct tgaggatcgc
     5161 cgcccccatg ccgcggtcga tgacgccgtc ccgggcggct tgcaggagga aggcgttgat
     5221 gtccttggtc gagatcgtca cagctgatgc acctgtccat cgcgggccga agtggatcga
     5281 tcggggctgg agggtcgagc cagaccgcgc atgaggatcg agcacagcgg ggcgacgtcg
     5341 tcgaaccagt agaagtggcc accgggaagc tgatggacat cgaagggtcc gtcggtgaac
     5401 tcaccccacc gccgaagctg ctcagccgag acgacggggt cccgagtgcc ggccactgcg
     5461 gtcaccgggc agtgcaggag ttcggcggga gcggcgtact cgtaggtctc ggccaccttg
     5521 tagtcggact tgaggatcgg ggtgaacaac tcgacgagct cgggggtgtc caacacctgt
     5581 tgggaggtgc cgccgaagcc acggatcatc gagaggaaga ccgaatccgg tgcctggtgc
     5641 cacgacgtct tggacggaac gtcaggcgta tgccgtccgg agaagaacac gtgaaccggc
     5701 tcgatgcctt cgtgctcctg cagggaatgc agcacttcga aggccagcag ggtgcccacg
     5761 ctgtggcaga acacggcgaa cggtccggcc
//
LOCUS       DAOMZY010000237         5775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_13116_length_5775_cov_5.643007, whole genome shotgun sequence.
ACCESSION   DAOMZY010000237 DAOMZY010000000
VERSION     DAOMZY010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13116_length_5775_cov_5.643007"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="PLK64_12440"
     CDS             complement(<1..1081)
                     /locus_tag="PLK64_12440"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HPV80602.1"
                     /translation="MSRPLWFVGADGSVVSRALGVLRLSLLALAIGQGLVANAFATAS
                     LLPYLISLVVTGGILGSTLLPQLLPQFVTSDREDPASDADKTDDKTDDVQVAPVDEAG
                     STAPAPNGGANDAAPSLRFVQRLGTRLDIDRGLAIILVGLLLLTIVCIVGASTLVRGL
                     GSGLTGSLGDLTASFAAVTLVQVFLYGVCAVFGRVLAARRRLGALGWAPAIGELVGIG
                     GLLAFMRLFQGHVSSAEWTPEMVWWLAGAATAGIAVQALVLAFAVWRSGPGTATLVAA
                     WSVSRLFGGRSVTALVTVASYAVAAQVMWRATGYDEVSRGPGQTFVAGLASLVNALVV
                     VLLPHALLTRPALGSVHQRVAAGQGE"
     gene            complement(1078..2889)
                     /gene="menD"
                     /locus_tag="PLK64_12445"
     CDS             complement(1078..2889)
                     /gene="menD"
                     /locus_tag="PLK64_12445"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HPV80603.1"
                     /translation="MTITSAARAIHVLRGLLAAGVREVVLSPGSRSAPLALALHAADA
                     AGDVRLHVRLDERSAGFLALGLAVGSRSPVAVVMTSGTAVGNLLPAVMEAHHAGRPLI
                     VVSADRPASLRGTGANQTTWQDGIFGVFAPTVDVAVDASHDAVSQAVTQAGLRLGPSH
                     LNVQFDAPLLPDPGMPWWPGLLDASDPLDPSDLVDSSSARTSGGSLEEVPLGAEAVTL
                     VAGPRTVVLAGDDAGSAARVLAESAGVPLLAEPTSGSRAGDHAIRTYRLLLGTALGAA
                     IERVIVIGHPTLSRPVTALLSRPDIALYAVRTRTGVATDPARRAIILDVPPALTPALP
                     LGQTPGLAPDADAKWLTAWQAADAALAERVDAHVEALPDLHALHVAAEVSAALSPGQS
                     LVVGSSNPIRDLDLMLPPYRPGEHRLVVANRGLAGIDGTVSTAIGVALGRAGRIPPPG
                     RGIPPSPEDGGIPRPGRSLAVMGDLTFLHDSGGLLIGPDEPRPDLTIVVVNDDGGSIF
                     ALLEQGTPDYAAAFERVFATPVHADLQALAGASHTAYRRVESRVELREALSETATGPL
                     VIEARVGRADRRETAAALGALAADLPLSWPPDQGGMP"
     gene            complement(2914..4203)
                     /locus_tag="PLK64_12450"
     CDS             complement(2914..4203)
                     /locus_tag="PLK64_12450"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HPV80604.1"
                     /translation="MSTLHGPARAASGTVAPLVVRTVEIDDPGPLLALLPPAEQPGDV
                     LSWVRRGEGLVGWGQAATFTTAGPERFADADRWWRHTAARAVVRDEVGMPSSGLVCFG
                     SLPFADDSADRASFVVPRVSVGHKGSRWWVTTVGDVPTLAVTPAPTDDPLPPRNVAFA
                     DGALSSTAWAARVAEAVARINAGDLDKVVLARDLIVQADAPIDPRWVLRALADRYDNT
                     WVFSVSGMVGATPELLVRRERGLVTSRVLAGTIRRTGDDAKDLSLAASLARSSKDLEE
                     HEYAVRSVADALAPHCSSMNVPESPFVLHLPNVMHLATDVTGVATDDASSLALAASLH
                     PSAAVGGTPTPLAVALIAELEDMDRGRYAGPVGWMDAKGDGEWGIALRSGRYVDGDPA
                     SLRIFAGCGIVAGSDPDGEVSEADAKFVPMRDALSGR"
     gene            4311..5018
                     /locus_tag="PLK64_12455"
     CDS             4311..5018
                     /locus_tag="PLK64_12455"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HPV80605.1"
                     /translation="MTRADLDKAPQDVASMFDTVAERYDLTNDVLSAGQDRRWRKATV
                     AAVGAQPGQTVLDIAAGTGTSSEPFADAGVHVVPADFSLGMLRVGQRRRPDLAFTAAD
                     ATRLPFADASFDAVTMSFGLRNVVDVDGALREFARVTKPGGRLVVCEFSQPVWGPVRA
                     AYLNYLMRALPSIARKTSSNPESYVYLAESIRAWPDQRGLAARITDAGWADVQWRNLS
                     GGIVALHRATRPAPGAP"
     gene            5088..>5775
                     /locus_tag="PLK64_12460"
     CDS             5088..>5775
                     /locus_tag="PLK64_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPV80606.1"
                     /translation="MTTGSADQAGDTADVIVVGAGPGGSATAAYLATQGVDVMLLEKA
                     TFPRDKICGDGLTPRAVRELVHLGVDTAGWQRTRGLRILGGGRNFTLDWPESATFPSY
                     GMVRTRKQFDHALAQHAVSRGARLHEGASVTAPLLDGRGRVTGVTAKVLDDAGRATGA
                     TATYRAKVVVAADGVSSRLATAVGRPKREDRVMGVAVRAYYTTPRRDDYLESHLELWT
                     TDDNGQRMLMP"
BASE COUNT          882 a         2073 c         2108 g          712 t
ORIGIN      
        1 cttcaccctg ccctgcggcc acgcgctgat gcaccgaccc gagtgccggg cgggtgagca
       61 gcgcatgcgg cagtaggacg accaccaagg cgttgacgag gctcgccaga cccgccacaa
      121 aggtctgacc cggcccgcgg gagacctcgt cgtagcccgt ggctcgccac atgacctggg
      181 ctgcgacggc gtaactcgcc acggtcacca gggccgtcac cgagcgcccc ccgaacagcc
      241 gcgacacgct ccaggcagcc acgagggtgg ccgtgcccgg cccgctgcgc cacacggcga
      301 acgcgagcac caacgcctgc acggcgattc ccgcggtggc cgcacccgcg agccaccaca
      361 ccatctcggg ggtccactcc gcggaggaga cgtgcccctg gaagagtcgc atgaaggcca
      421 gcagcccgcc gatgccgacg agttcgccga tggcgggagc ccagcccaac gcgccgagcc
      481 gccgacgggc ggcgagcacc cggccgaaga cggcgcagac gccgtagagg aagacctgga
      541 ccagggtgac cgctgcgaac gaggcggtca gatcgccgag gctgccggtg agcccgctgc
      601 ccagtccgcg cacgagggtg ctcgccccca cgatgcacac gatcgtcagc agcagcagcc
      661 cgacgaggat gatcgccagc cctcggtcga tgtccaggcg ggtccccagc cgttggacga
      721 agcggaggga aggcgccgcg tcattggcac caccgtttgg tgccggggcg gtcgagccgg
      781 cttcgtcgac aggcgcgacc tggacgtcgt cggtcttgtc gtcggtcttg tctgcgtcgc
      841 tagccgggtc ctcccggtcg gacgtgacga actgcggcag gagctggggc aacaacgtcg
      901 agccgaggat gccgccggtg acgaccagcg agatgagata gggcagcagg ctcgccgtgg
      961 cgaacgcgtt ggccacgagg ccttgcccga tagccagcgc caggagtgag agtcgaagga
     1021 cgcccaacgc ccgggacacg accgacccgt ccgccccgac gaaccacaag gggcggctca
     1081 cggcataccc ccttggtcgg gtggccacga caacggcaag tcggcagcga gtgccccgag
     1141 cgcggcagcg gtctcgcgcc ggtccgcgcg ccccacccgc gcctcgatga ccaggggtcc
     1201 ggtggcagtt tcgctgagcg cctcgcgcag ctcgacgcga gactcgaccc gtcggtatgc
     1261 cgtgtggctg gcgcccgcga gcgcctgcag gtcggcgtgc accggcgtcg cgaacacccg
     1321 ctcgaaggcg gctgcgtagt caggggtgcc ctgctcgagc agcgcgaaga tcgagccgcc
     1381 gtcgtcgttg acgacgacga tcgtcaggtc gggacggggt tcgtccggac cgatgagcag
     1441 ccccccgctg tcgtgtagga aggtcaagtc gcccatgacg gccaggctcc tgcccggtcg
     1501 aggtattccg ccatcctcag gcgatggcgg aatacctcga cccggcgggg gaatgcggcc
     1561 ggcccggccc agcgcgaccc cgatggcggt cgagacagtg ccgtcgatgc cggccaggcc
     1621 ccggttggcg acgacgagcc ggtgttcgcc tggccgataa ggggggagca tgaggtccag
     1681 gtcgcggatc gggttggacg agccgacgac gaggctctga cccggcgaca gggccgcgga
     1741 cacctcggcc gccacgtgca gggcgtgcag gtcgggcagg gcctcgacgt gcgcgtcgac
     1801 ccgttcggcc aacgcggcat cggcggcctg ccacgcagtc agccacttcg cgtcggcgtc
     1861 cggggccagt cccggggtct gtcccagagg cagcgccggc gtcagtgccg gtggcacgtc
     1921 gaggatgatc gcgcgtcggg ccgggtcggt ggcgaccccc gtgcgggtgc ggacggcata
     1981 cagggcgatg tcgggccgtg acagcagcgc ggtgacgggg cgcgagaggg tcgggtgtcc
     2041 gatgacgatg acccgctcga tggcggcccc gagcgcggtg ccgagcaaca gtcgataggt
     2101 gcggatcgcg tggtcgccgg cccgcgatcc ggaggtcggc tcggcaagca gcggcacccc
     2161 ggcggattcg gcgaggacgc gagcggccga cccggcatcg tccccggcca gcacgacggt
     2221 tcgcggccct gccaccaacg tcacggcctc ggcgccgagg ggcacctcct ccagcgatcc
     2281 gcctgaggtg cgcgccgacg acgaatcgac caggtcggaa ggatcgagag ggtcggacgc
     2341 gtcgaggagg ccgggccacc acggcatacc cggatcaggg agcagcggcg catcgaactg
     2401 gacgttgagg tgcgacggcc cgagccgcag ccccgcctgc gtgaccgcct gcgacacggc
     2461 gtcgtgcgac gcgtcgaccg cgacatcgac cgtcggtgcg aacactccga agatgccgtc
     2521 ctgccaggtc gtctggttgg cccccgtgcc gcgcagcgac gccgggcgat cggccgacac
     2581 gacgatgagc ggccgcccgg cgtggtgggc ctccatgacg gcgggcaaca gattgccgac
     2641 ggccgtcccc gaggtcatga ccaccgccac cggtgaccgc gatccgaccg ccaaacccag
     2701 cgcgaggaac ccggcggagc gctcgtcgag ccgcacgtgc aggcgcacat cgccggctgc
     2761 gtcggcggca tgcagcgcga gggcgagcgg cgccgagcgg gagccgggag agaggacgac
     2821 ctcgcgcacc cccgcggcga gaaggcctcg cagcacgtgg atggcgcgcg ccgcgctcgt
     2881 gatcgtcatc aggacgagta tgccgcgcgc gggctaacga ccgctgagcg cgtcgcgcat
     2941 ggggacgaac ttggcgtcgg cctcgctgac ctcgccgtcc gggtcggacc cggcgacgat
     3001 gccgcagccg gcgaagatcc gcagcgatgc cgggtcgccg tcgacatagc gacccgagcg
     3061 cagcgcgatg ccccactcgc cgtcgccctt ggcgtccatc cacccgacgg gcccggcgta
     3121 acgaccccgg tccatgtcct cgagctcggc gatcaacgcc actgcgagcg gggtcggggt
     3181 gccgccgacg gcggccgacg ggtgcagcga cgcagccagc gccagcgagg acgcatcgtc
     3241 ggtcgcgacc ccggtgacat cggtggccaa gtgcatgacg ttgggcaggt gtaggacgaa
     3301 cggcgactcg gggacgttca tcgacgagca gtgcggggca agggcatccg cgaccgagcg
     3361 cacggcgtat tcgtgctcct caaggtcctt gctggagcgg gccagcgacg cggcgagcga
     3421 gagatccttg gcgtcgtcgc ccgtgcgtcg gatcgtcccg gcgagcactc gcgaggtgac
     3481 cagcccgcgc tcgcggcgaa cgaggagttc gggggtcgca ccgaccatgc cggagaccga
     3541 gaacacccag gtgttgtcgt agcgatcggc caacgcccgc agcacccacc gcgggtcgat
     3601 cggcgcgtcg gcctgcacga tgaggtctct ggcgaggacg accttgtcga ggtcgcccgc
     3661 gttgatccgc gcgaccgcct cggccacgcg ggcagcccac gcggtgctcg acagggcacc
     3721 gtcggcgaag gccacgttgc ggggcggcag cgggtcgtcg gtcggggccg gcgtgacggc
     3781 aagcgtcggg acgtcgccga ccgtggtcac ccaccaccgg ctgcccttgt ggccgacgct
     3841 gacccgcggg acgacgaagc tggcgcggtc ggcggagtcg tccgcgaacg gcaggctccc
     3901 gaagcagacg agcccgctgg acggcatacc cacctcgtcg cggaccacgg cgcgagcagc
     3961 ggtatgccgc caccaccggt ctgcgtccgc gaagcgctcg ggtccggccg tcgtgaacgt
     4021 ggcggcctgc ccccacccga cgaggccctc gccgcgccgg acccacgaca acacgtcgcc
     4081 cggttgctcg gcgggaggga ggagggccag cagcggcccg gggtcgtcga tctccaccgt
     4141 gcgcacgacg agcggcgcga cggtgccgga ggcggcccgg gcgggcccgt ggagggtgga
     4201 catcgaaggt caatgttacg actcggtatg ccgcccgccc gccgcccacg cgccgcccgc
     4261 cacagtgcgg gtgctgggga ggggtcgcgg agggtggaag gatgccagcc atgacccggg
     4321 ccgatctgga caaggcaccc caggacgtcg cgtcgatgtt cgacacggtg gccgagcgtt
     4381 acgacctcac caacgacgtc ttgtcggcgg gacaggaccg gcgttggcgc aaggccacgg
     4441 tcgcggccgt cggcgcccag cccggccaga cggtgctcga tatcgccgcg gggaccggga
     4501 ccagctccga gccgttcgct gacgcagggg tccacgtcgt gccggccgac ttctcactgg
     4561 ggatgctgcg ggtcggccag cgacggcgcc ccgacctggc cttcactgcg gccgacgcga
     4621 cccggttgcc cttcgctgac gcgtccttcg acgcggtgac catgtcgttc gggctgcgca
     4681 acgtcgtcga tgtcgacggg gcgctgcggg agttcgcccg ggtgaccaag ccggggggtc
     4741 gcctcgtcgt gtgcgagttc agccagccgg tgtggggtcc ggtgcgggcg gcatacctca
     4801 actatctgat gcgggcgctg ccctcgatcg cgcggaagac gtcctccaac ccggagtcct
     4861 acgtctacct cgccgagtcg atccgcgcct ggcccgacca gcggggactc gcggcgcgga
     4921 tcaccgacgc cggctgggcc gacgtgcagt ggcgcaacct cagtggaggc atcgtcgctc
     4981 tccaccgcgc gacccgcccc gcccccggtg ccccctagac tgggcgggtt cgtgccattc
     5041 ttcacaagcg gcgccttcac cggcgcccca ccgcaccgag ggcgagagtg accaccggat
     5101 cggctgacca ggcgggcgac accgccgacg tcatcgtcgt cggagccggg ccaggaggct
     5161 cggccacggc ggcctacctg gctacccagg gcgtcgacgt catgctcctg gagaaggcga
     5221 cgttcccacg ggacaagatc tgcggtgacg gcctcacgcc ccgcgccgtg cgcgagttgg
     5281 tccacctcgg cgtcgacacg gccgggtggc aacgcacccg cggcctgcgc atcctcggcg
     5341 gcggccgcaa cttcaccctc gactggcccg agagtgcgac cttcccgtcc tatggcatgg
     5401 tccgcacccg caagcagttc gaccacgcgc tcgcgcaaca cgccgtctct cggggcgccc
     5461 gactgcatga aggcgccagc gtcaccgcgc ccctgctcga tggtcgtggc cgggtcaccg
     5521 gcgtcaccgc caaggtgctc gacgacgcgg gtcgcgccac cggagcgacc gcgacctacc
     5581 gcgccaaagt cgtcgtcgcc gcggacgggg tctcctcgcg cctggccacg gcggtcggcc
     5641 gccccaagcg cgaggaccgc gtcatgggcg tggcggtccg cgcctactac acgacccctc
     5701 ggcgcgatga ctaccttgag tcgcacctcg agctctggac caccgacgac aacggtcagc
     5761 ggatgctcat gccgg
//
LOCUS       DAOMZY010000238         5771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_13131_length_5771_cov_8.404479, whole genome shotgun sequence.
ACCESSION   DAOMZY010000238 DAOMZY010000000
VERSION     DAOMZY010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13131_length_5771_cov_8.404479"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..1372
                     /gene="xseA"
                     /locus_tag="PLK64_12465"
     CDS             173..1372
                     /gene="xseA"
                     /locus_tag="PLK64_12465"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPV80607.1"
                     /translation="MRVLSLKIRDYVDKMSAMWVEGQVVQLQRRGNTAYLTLRDPEVD
                     MSLSATISVHALNAMGSPLLEGARVVLQAKPTFWERRGTLQLDGRQIRPVGVGDLLAR
                     LEILKARLAAEGLFDRDRKRRLPFLPRRVGLITGRDSAAERDVVENARRRWPAVQFEI
                     REVAVQGTSAVAEVTAALVELDRLAGVDVIVIARGGGAVEELLPFSNETLLRAVAAAA
                     TPVVSAIGHDVDTPLLDFVADVRASTPTDAAKLVVPDAAAERDRVAALHGRARLAVRH
                     RLDVERRLLVAHRTRAVMTDPLALLTPHRSQLAALRGRATDRLVGRLEREGDRISHLR
                     AQVRALSPQSTLDRGYAVVTHPDGRVVRDREDVAPDELLRVLVARGDFGVRVIGHESP
                     PKHAAPS"
     gene            1472..1720
                     /locus_tag="PLK64_12470"
     CDS             1472..1720
                     /locus_tag="PLK64_12470"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015776297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPV80608.1"
                     /translation="MPTDPASKADPADLADLTYEQARDELITIVAQLESGQVPLEQSM
                     SLWHRGEALAAYCAHWLDDAQSKIADAGVVMRIRDDEA"
     gene            complement(1782..2354)
                     /locus_tag="PLK64_12475"
     CDS             complement(1782..2354)
                     /locus_tag="PLK64_12475"
                     /inference="COORDINATES: protein motif:HMM:NF025396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4245 domain-containing protein"
                     /protein_id="HPV80609.1"
                     /translation="MTTPSVPSSGAQPRSRYSMGSGANMARSLLVVLALVAGLIALVP
                     RVDSVRQPAVDAASVVSYAVKDSGTPFSFPAGLPSGWTATSARFATSTDSRPTWQAGW
                     TTPAGQFVGLRQTVDATPAWVATGITGGKVTGSAEVAGRTWEIRTDERNQLYAVSVSA
                     DKLTTVVSATGGMDDIRYFIERLEPAKAAS"
     gene            2470..3405
                     /locus_tag="PLK64_12480"
     CDS             2470..3405
                     /locus_tag="PLK64_12480"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HPV80610.1"
                     /translation="MAERTAVLVVGEALIDAVVKGGEITGEHVGGSPANVAFGVGALG
                     HDVSLATWIGHDEHGDRILARCADAGVTVVPGSTGAERTSVAYASIDARGQATYRFDL
                     TWDVPELGDTSEIGHVHTGSIAATIEPGGSQVVEVIRRLRAQATVSYDPNMRPSLMGS
                     PDEVRARVEDLIALSDVVKASDEDIQWLYPGAFLPDVLRLWGELGAAVAVITRGGQGA
                     VYVVRSATTAEVTTVPAKATTVIDTVGAGDSFMAGLVSGLLDAGYLGGPDARERLRAA
                     GIDDLRPCVDRATATSGVTVGHAGAYAPTRAELGI"
     gene            3469..5154
                     /locus_tag="PLK64_12485"
     CDS             3469..5154
                     /locus_tag="PLK64_12485"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPV80611.1"
                     /translation="MVEFAYEDLLPIGADETPWRLLTTEGVRTVEGPGGRQFLEVDDE
                     AMRTLTETAMHDIAHYLRPAHLAQLRKILDDPEASNNDKFVALDLLKNANIAAGGVLP
                     MCQDTGTAIVMGKRGQHVLTGGTDERAISRGVYDAYTRLNLRYSQMAPLTMWDERNTG
                     SNLPAQIELYADTAPGHEAAYKFLFMAKGGGSANKSYLYQETKAILNPTGLMRFLDEK
                     LRSLGTAACPPYHLAIVIGGTSAEFALKTAKYASTKYLDTLPTEGSMTGHGFRDTALE
                     EQVLELTRQFGIGAQFGGKYFCHDVRVIRLPRHGASLPVAVAISCSADRQCLGKITPE
                     GVFIEQLEFEPGHYLPEPTAEQLAGDDAATDDVVRIDLSRPMSEILAELTRQPVKTRL
                     SLTGTLVVARDIAHAKIKERLDAGEGMPQYLKDHPVYYAGPAKTPAGMASGSFGPTTA
                     GRMDSYVDQFQAAGGSMVMLAKGNRSQQVTDACAAHGGFYLGSIGGPAARLAQDCIKS
                     VEVLEYPELGMEAVWKIEVEDFPAFVVVDDKGNDFFASVTKPVAFTIEKRPGL"
     gene            5172..>5771
                     /locus_tag="PLK64_12490"
     CDS             5172..>5771
                     /locus_tag="PLK64_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HPV80612.1"
                     /translation="MARHRPQPGTRPETPTEAWAVLSAGNARFMAGEREHPNQDVAIR
                     HRLAGKQQPFAIFFGCADSRVAAEIIFDRGLGDLFVVRTAGHTVDSSVLGSMEFGVGV
                     LGIPLIVILGHDSCGAVNAAVDAMTSGDLPGGFVRDIVERVMPSVLAASKHGEVGIDQ
                     IEAEHVRQTAALVAQRSHLIADHIAAGSPGIIGATYNLRD"
BASE COUNT          912 a         2098 c         1914 g          847 t
ORIGIN      
        1 cgcgcgctcg tcgggaaggc ggaatacagt cgcggttgtg agtatgccgc ccgctcccga
       61 cccgcgatcc tcgtcgcgcg cgtcgggctc accggcgccc gcgagcgcgc ccggcgcccg
      121 ggcagcgctg ccggagaagg cagccgacac gaccgcggag acaccctggc cggtgcgggt
      181 gctcagcctc aagatccgcg actacgtcga caagatgtcg gcgatgtggg tcgagggcca
      241 ggtcgtgcag ttgcagcggc ggggcaacac ggcatacctc accctgcgcg accccgaggt
      301 cgacatgtcg ctgtccgcga ccatctcggt ccacgcactc aacgccatgg gcagccccct
      361 cctcgaaggt gcgcgcgtcg tgctgcaggc caagccgacg ttctgggagc ggcgcgggac
      421 cctgcaactc gacggtcggc aaatccggcc ggtcggcgtg ggcgacctgc tggcccggct
      481 cgagatcctc aaagcgcgcc tggccgccga gggcttgttc gatcgggatc gcaaacgccg
      541 gctgccgttc ctgccccgcc gggtcggact gatcactggc cgtgacagcg cggccgagcg
      601 tgacgtcgtc gagaacgccc gacgccgttg gcccgcagtg cagttcgaga tccgcgaggt
      661 agccgtccag gggacctccg cggtcgccga ggtgacggcc gcgctcgtcg agctggaccg
      721 actcgcggga gtcgacgtca tcgtcatcgc ccgtggcggc ggcgccgtcg aggagttgct
      781 gccgttcagc aacgagaccc tcctgcgcgc cgtggccgcc gccgcaacgc ccgtggtgtc
      841 cgcgatcggt cacgatgtcg acaccccgct gctcgacttt gtcgccgacg tgcgcgcgtc
      901 cacgccgacc gatgccgcga agttggtcgt ccctgatgcg gccgccgaac gcgaccgggt
      961 cgctgccctc cacggtcgag cccggctcgc cgtgcgccat cggctcgacg tcgagcggcg
     1021 gctgctcgtc gcccaccgca cccgcgcggt gatgaccgac ccgctcgccc tgctcacccc
     1081 gcatcgcagc caattggcgg ccttgcgcgg acgcgcgact gatcggctcg tcggtcgcct
     1141 cgaacgcgaa ggcgaccgga tcagccacct ccgcgcccag gtgcgcgccc tctccccgca
     1201 gtcgactctc gaccgggggt atgccgtcgt cacccacccc gacgggcggg tcgttcgcga
     1261 ccgggaggac gtcgctccgg acgaactcct gcgggtcctg gtggctcgcg gcgacttcgg
     1321 ggtccgtgtc atcggccacg agtcgccgcc gaagcacgcg gcaccgtcgt agtcagggcg
     1381 tcgcgcgctg acggctgtcg aggaccacgg gatcgtctgc aaccgggcta aggtgttccc
     1441 gctctccatc ccgagcacga gaggcaaccc tgtgcccacc gacccggcca gcaaggccga
     1501 cccggccgac ctcgctgacc tgacctacga gcaggctcgc gacgagctca tcacgatcgt
     1561 ggcccagctc gagtcgggcc aggtgccgct ggagcagagc atgtcgctgt ggcaccgcgg
     1621 cgaggcgctt gcggcctact gcgcccactg gttggacgat gcccagtcca agatcgccga
     1681 tgcgggcgtc gtcatgcgga tccgcgacga cgaggcgtga gcgcgcgtca ccacgaagac
     1741 gagcgctccg ccacgagtga gaagtgcgtc gccgcctgcg ctcagctcgc ggctttggct
     1801 ggctcgagcc gttcgatgaa gtagcggatg tcgtccatgc cgcccgtggc gctgaccacc
     1861 gtcgtgagct tgtcggccga gactgacacg gcatacagct gattgcgctc gtccgtgcgg
     1921 atctcccagg tgcgccccgc gacctcggcg ctgccggtga ccttgccgcc cgtgatgccg
     1981 gtggcgaccc aggccggcgt cgcgtcgaca gtctggcgca acccgacgaa ctgccccgcc
     2041 ggcgtcgtcc aacctgcctg ccaggtcggc cgcgagtcgg tcgaggttgc aaagcgggcg
     2101 ctcgtggccg tccaccccga ggggagcccg gcggggaacg agaacggcgt gccgctgtcc
     2161 ttcacggcat acgagacgac cgatgcggca tcgacggccg gctggcggac cgagtcgaca
     2221 cggggcacca acgcgatcaa ccccgccacg agggcgagca cgaccagcag cgatcgggcc
     2281 atgttggcac cgctgcccat gctgtagcgg ctgcgcggct gcgcgcctga cgagggcacc
     2341 gagggtgtgg tcactccccc atcgtccccg accggacaga ccgcaaccaa accgggatgt
     2401 ggaccagtgc cgagtcgcga gcgggcagcc ctaggctgag gcgctatcag ctactcccag
     2461 gaggaaaccg tggccgaacg cactgccgtt ctcgttgtgg gcgaggccct catcgacgcc
     2521 gtcgtcaagg gcggcgagat caccggcgag catgtcggcg gcagtccggc caacgtcgcg
     2581 ttcggcgtcg gcgctctcgg tcacgacgtc tcgctagcca cctggatcgg ccacgacgag
     2641 cacggtgacc gcatcctcgc gaggtgcgcc gacgcgggcg tcaccgtcgt tcccggcagc
     2701 accggagcag agcgcaccag cgtcgcctac gcgtccatcg acgcgcgcgg acaggcgacc
     2761 taccgcttcg acctcacctg ggacgtcccc gagctcggtg acaccagcga gatcggccac
     2821 gttcacaccg gaagcatcgc cgcgaccatc gagcccggcg gcagccaggt cgttgaggtc
     2881 atccgtcgcc tgcgtgctca ggccacggtc tcctacgacc ccaacatgcg cccctcgctc
     2941 atgggcagcc ccgatgaggt ccgggcccga gtcgaggacc tcatcgcgct ctccgacgtc
     3001 gtcaaggcca gcgacgagga catccagtgg ctctacccgg gcgccttcct gcccgacgtg
     3061 ctgcgcctct ggggcgagtt gggtgccgcg gtcgccgtca tcacccgcgg cggccagggc
     3121 gccgtctatg tcgtgcgctc ggcgacgacg gctgaggtca ccacggtccc cgccaaggcg
     3181 acgaccgtca tcgacacggt cggtgccggt gactccttca tggccgggct ggtgtccggg
     3241 ctgctggacg ccggctacct cggcggtccg gatgcccgag agcggctgcg cgctgccggg
     3301 atcgatgacc tgcgcccctg tgtggatcgg gctaccgcga ccagcggcgt gaccgtcggc
     3361 cacgccggcg cctacgcacc gacccgggcc gagctaggca tctgagcgcc cctcctatgc
     3421 tgggggtgaa gtgcacaacg gactgcccct aacgacgtgg gagtgatgat ggtcgagttc
     3481 gcctatgagg atctgctgcc gatcggcgcg gacgagacac cctggcgatt gttgacgacc
     3541 gagggtgtcc ggacggtcga aggacccggt ggtcggcagt tcctcgaggt cgacgacgag
     3601 gcgatgcgca ccctcacgga gacggcgatg cacgacatcg cccactacct gcgcccggct
     3661 cacctggctc agttgcgcaa gatcctcgat gaccccgagg ccagcaacaa cgacaagttc
     3721 gtcgcgctcg acctgctgaa gaacgccaac atcgccgccg gcggtgtcct gccgatgtgt
     3781 caggacaccg gcaccgccat cgtcatgggc aagcgcggcc agcacgtcct caccggcggc
     3841 accgacgagc gggcgatcag ccggggcgtc tacgacgcct acacccggct caacctgcgc
     3901 tacagccaga tggcaccgct gacgatgtgg gacgagcgca acaccggctc caacctcccg
     3961 gcccagatcg agttgtatgc cgacacggct cctggacacg aggcggcata caagttcctc
     4021 ttcatggcca agggcggcgg gtcggcgaac aagagctacc tctaccaaga gaccaaggcg
     4081 atcctcaatc ccaccggcct catgcgcttc ctcgacgaga agctgcgttc gctcggcacg
     4141 gcggcctgcc cgccctacca cctggccatc gtcatcggcg ggacctccgc tgaattcgcg
     4201 ctcaagacag ccaaatacgc gtcgacgaag tacctcgaca cactgccgac cgaagggtcc
     4261 atgacaggcc acggcttccg cgacaccgcg cttgaggagc aggtcctcga gttgacccgc
     4321 cagttcggca tcggcgccca gttcggcggc aagtacttct gtcacgacgt gcgcgtcatc
     4381 cggctcccgc ggcacggcgc cagcctgccg gtggcggtcg ccatttcgtg ttcggccgac
     4441 cggcagtgcc tgggcaagat cacccccgag ggcgtcttca tcgagcaact cgagttcgag
     4501 cccggtcact acctgcccga gccgaccgcc gaacaactcg caggagacga cgccgcgacc
     4561 gacgatgtcg tgcgcatcga cctgtcccgg ccgatgtccg agatcctcgc cgagctcacc
     4621 cggcagccgg tcaagacccg gttgtcgctc accgggacgc tcgtcgtcgc ccgtgacatc
     4681 gctcacgcca agatcaagga gcgtctcgac gccggcgagg ggatgccgca gtacctcaag
     4741 gaccacccgg tctactacgc gggcccggcc aagaccccgg ccggcatggc ctcagggtcg
     4801 ttcggcccga cgacggccgg ccggatggac tcctacgtcg atcagttcca ggcggccggt
     4861 gggtcgatgg tcatgctcgc caaaggcaac cgcagccagc aggtcaccga tgcctgcgcc
     4921 gcccacggcg gcttctatct cggctcgatc ggcggccccg cggcgcgcct ggcccaggac
     4981 tgcatcaaga gcgtcgaggt gttggagtac cccgagttgg gcatggaagc ggtctggaag
     5041 atcgaggtcg aggacttccc ggcattcgtc gtcgtcgatg acaagggcaa cgacttcttc
     5101 gcgtccgtga ccaagcccgt cgccttcacc atcgaaaagc gcccggggct gtaacggcat
     5161 acgctcgggg tgtggcacga caccgacccc agccgggcac gcgaccggaa acaccgaccg
     5221 aggcgtgggc agtcctgtct gccggaaacg cccgcttcat ggctggcgag cgcgagcacc
     5281 ccaaccagga tgtggccatc cggcaccgcc tcgccggcaa gcaacagccc ttcgcgatct
     5341 tcttcggatg cgccgactcg cgggtcgcgg ccgagatcat cttcgaccgc ggcctgggcg
     5401 atctcttcgt cgtccgcacc gcgggccaca ccgtcgactc cagcgtcctg gggtcgatgg
     5461 agttcggggt cggcgtcctc ggcatcccgc tgatcgtcat ccttggccat gactcgtgcg
     5521 gcgcggtcaa cgctgccgtc gacgccatga ccagtggcga tctgcctggc ggtttcgtgc
     5581 gcgacatcgt cgagcgcgtc atgcccagcg tgctcgccgc gagcaagcac ggcgaggtcg
     5641 ggatcgacca gatcgaggcc gagcacgtgc gccagaccgc agccctggtt gcgcaacgct
     5701 cccacctcat cgccgaccac atcgccgccg ggtcgcccgg catcatcggc gcgacctaca
     5761 acctgcgcga c
//
LOCUS       DAOMZY010000239         5755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_13175_length_5755_cov_5.386491, whole genome shotgun sequence.
ACCESSION   DAOMZY010000239 DAOMZY010000000
VERSION     DAOMZY010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5755
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13175_length_5755_cov_5.386491"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PLK64_12495"
     CDS             <1..247
                     /locus_tag="PLK64_12495"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HPV80613.1"
                     /translation="AWLRTELRRVSARGYAVNRETSEKGVTAVGRAVRDESGVAVAAV
                     SISMPSARYAATDLPRITGALALAARAIEADLSADRP"
     gene            complement(248..973)
                     /locus_tag="PLK64_12500"
     CDS             complement(248..973)
                     /locus_tag="PLK64_12500"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPV80614.1"
                     /translation="MSSSEIDGQADERGACANEFGQRVGAPVDWQPATPPRPVTLAGR
                     WCTVVPLDLDHVPDLYAALAPGPPGQWTYMTFGPCDTEADLREVVSGYLANPGFVSFA
                     ILDAAGRARGIAAYLRIDPAIGSLEVGGILLGEGLRRTAAATEAMWLLARNAFEQLGF
                     RRYEWKCDALNTPSRYAAERLGFRFEGVWRQATIYKGRNRDTAWFAMTDADWPEVSSA
                     IQAWLDPTNFDAAGRQLTPLRIG"
     gene            complement(980..1678)
                     /locus_tag="PLK64_12505"
     CDS             complement(980..1678)
                     /locus_tag="PLK64_12505"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HPV80615.1"
                     /translation="MSERVLIPVADGELPAYLWVPESGTGPGVVLVQEIFGLSPYIHR
                     RAADLAALGYVVLAPQVFWRLGAESVPDGPGMLEEGIGLVSRLDWPAAVGDMVAAFEW
                     LRARPEIGGAGVVLVGFCFGGGLAYNVAVETPVDALVSYYGSALPGLVDILPVVEAPS
                     LHHFGEADSFIDGPTVERIRGVVGQSAACEFHTYPGADHAFDNPDFVLHHPQASALAW
                     ERTVEFLGRVLSVS"
     gene            1747..3036
                     /locus_tag="PLK64_12510"
     CDS             1747..3036
                     /locus_tag="PLK64_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV80616.1"
                     /translation="MTVRDRLRSLRVDTTPLRTSPDFRRLFFAGTVFYLGGMVSYVAL
                     PWQLYHLTGSNLAVGLLGVVELVPLVAFGLWGGALADHVDRRLMLVWTGVAQVVLTAL
                     LMLNAALPEPQTWAIYAVGAVLSAAGALQRPSREALIPRTVRHDELPAAVSVSSLGVQ
                     VGLLGGPALGGILVESVGAAAAYAIDVAGLIIATALFARLGSYPPSEDATPASLRSIA
                     EGARYAVSRRDLLGTYVIDIVAMFMAMPMVLFPALANDVLKSPHTLGLLYSAGTVGSM
                     LVTATSGWTSRVHRHGMAVVVSAAVWGAAVAAAGLTTNVPIVLLCFAIAGAADMVSAL
                     FRGVIWHQTIPDSMRGRLAGIEMLSYSVGPLGGQVRSGLVADRWSVRASITSGGVLCV
                     IGVVATAAWLRDFWSYDARTDPYAAAERQRRAADGRD"
     gene            3094..3957
                     /locus_tag="PLK64_12515"
     CDS             3094..3957
                     /locus_tag="PLK64_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPV80617.1"
                     /translation="MASDAARTTGRTERLTQFQRDGLTFDVTDSGPLDGPVVVLLHGF
                     PTDRSSWDRISPLLHAAGLRTLAPDQRGYSPGARPQGRAAYRLGEVADDVVALADAVG
                     ADAVHVVGHDWGGAIAWIVAGRHPERVASLTALTTPHPFAMTEALRSRDFDQARRSWH
                     MLAFQVPVIPDRYLASRLAAVLRPSGLPAEDATRYAARFSEPGALTAALNWYRAIPLS
                     SGVVHSCRVPTTYVWGRKDPFLGPVAARLTAHSVLADYELVELDEGHWLPERAPQACA
                     RAIIARVASVD"
     gene            4052..4975
                     /locus_tag="PLK64_12520"
     CDS             4052..4975
                     /locus_tag="PLK64_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015937246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPV80618.1"
                     /translation="MTWARGMPVAPKIPTPADDLRRPAPPGRETPPRFGGVDFDSLPD
                     LIPDDTAYYRRISADTFLPTLNTQGAWNAHEQHMAPVSGLLAHALTTHEPRPELALAR
                     ITYEILGLIPAIPTTIEVRTIRPGRTIELIEATASTGGRPVVRATAWRLSRQDTTEVA
                     GGEPSPLPSPDDCVPWRPSEIWPGGYIASIELRHTPDKQPGTGRAWIRTDKAVVADEE
                     VAPIAAYLGLVDTANGMNVRVDPSEWLFPNIDLTVHLHRDPLGGPGHWVGFDTQVTFG
                     REGVGLTSTTLHDESGAVGRAEQILTLRAGR"
     gene            4989..>5755
                     /locus_tag="PLK64_12525"
     CDS             4989..>5755
                     /locus_tag="PLK64_12525"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HPV80619.1"
                     /translation="MPSAPPHPLLPWIEGYAVRLGSPLTSPTAWVTTASGAELAFVGG
                     LVVKLHHPRTDVAELRTRLALATDPALAAYLVLPVTSEVVVAPDGRAVTAWPRVEVLD
                     PADADSEGATAPWTDAGRLLAGLHRARPLGSMPVHGGWARLRRAVVRAEGGADVDPRL
                     GMVAAAGQRVLAALDLAAADAAPRPDRHLTVVHGDWHLGQLGRVDGAWRLLDIDDLGW
                     GDPAWDLARPAGFWAAGLLPTDDWEGFLAAYRAAGGPG"
BASE COUNT          790 a         2167 c         1940 g          858 t
ORIGIN      
        1 cgcgtggctg cgcaccgaac tgcgccgcgt gtcggccagg gggtatgccg tgaaccgcga
       61 gacctccgag aagggcgtca ccgccgtcgg gcgcgcggtg cgcgacgagt cgggcgtggc
      121 cgtcgccgcc gtttcgatct cgatgcccag tgcccggtat gccgccaccg acctcccccg
      181 catcaccggg gccttggcgt tggccgcccg agccatcgaa gccgacctct ccgccgaccg
      241 cccgtagtca gccgatccgc aggggggtga gctgtcggcc agcggcgtcg aagttggtcg
      301 ggtcgagcca ggcctggatc gcggagctca cctcaggcca gtcggcgtca gtcattgcga
      361 accacgccgt gtcccggttg cgacccttgt agatcgtcgc ctgccgccac accccctcga
      421 agcggaagcc gagccgctcg gcggcatacc tgctgggggt gttgagcgcg tcacacttcc
      481 actcgtagcg tcgaaaaccc agctgctcga acgcattccg cgcgagcagc cacatcgcct
      541 cggtcgccgc agccgtgcgc cgcagcccct cgccgagcag gatcccgccc acctccagcg
      601 agccgatggc cgggtcgatc cgcaggtatg ccgcgatccc ccgtgctcgt ccggccgcgt
      661 cgaggatcgc gaacgacacg aaaccggggt tggcgagata gcccgacacg acctcgcgca
      721 ggtccgcttc ggtgtcgcac ggcccgaacg tcatgtaagt ccactgcccg ggcgggccgg
      781 gggcgagggc ggcatacagg tcggggacgt ggtcgaggtc gagcgggacg acggtgcacc
      841 accggccggc gagggtgact gggcgaggcg gagtggcggg ttgccagtcg accggcgcac
      901 cgacgcgttg cccgaattcg ttggcgcacg cgccccgttc gtcggcttgg ccgtcaattt
      961 cgctgctgga caacgccgtt caactcaccg atagaacgcg cccgaggaac tcgaccgtcc
     1021 gctcccacgc gagcgccgag gcctgcgggt gatgcagcac gaagtcgggg ttgtcgaagg
     1081 catggtcggc gcccggatag gtgtggaact cgcacgccgc cgactgcccc acgaccccgc
     1141 ggatccgctc gaccgtgggc ccgtcgatga acgagtcggc ctcgccgaag tggtgcaggc
     1201 tcggcgcctc gaccaccggg agtatgtcca ccagccccgg cagcgccgac ccgtagtaac
     1261 tcaccagcgc atcgaccgga gtctccaccg caacgttgta ggccagcccg ccgccgaagc
     1321 agaacccgac gaggaccacg cccgcgccac cgatttcggg ccgcgcgcgc agccattcga
     1381 acgcggcaac catgtcaccc accgctgcgg gccagtcgag gcggctgacc agcccgatgc
     1441 cctcctcgag catccccggc ccgtccggca ccgattcggc cccgagccgc cagaacacct
     1501 gcggcgccag cacgacatag ccgagcgcgg ccagatcggc ggcccgacga tgaatgtagg
     1561 gcgacagccc gaagatctcc tgaaccagga ccacccccgg cccggtcccg ctctccggca
     1621 cccagaggta tgccggcagc tcaccatcag cgacggggat cagcacgcgt tcgctcatgg
     1681 ccgctcagta tgccgcccgc gaaactcagg cgcgcggcat accttatctg cggcacactc
     1741 actcgggtga ccgtccgtga ccgcttgcgc tccttgcgcg tggacacaac cccgctacga
     1801 acctcccccg acttccggcg gttgttcttc gcggggacgg tgttctatct cggcgggatg
     1861 gtgtcctatg tggcgctgcc gtggcagctc taccacctca ccgggtccaa cctcgcggtc
     1921 gggctgctcg gggtcgtcga gctcgtgccg ctcgtcgcgt tcgggctgtg gggcggggcg
     1981 ctggccgacc acgtcgaccg tcggctcatg ctcgtgtgga ccggtgtcgc ccaggtcgtg
     2041 ctcaccgcgc tgctcatgct caacgcggcc ctgcccgagc cgcagacctg ggcgatctac
     2101 gccgtcggag cggtgctcag cgccgcgggc gcgctgcaac gcccgagccg cgaggcgctc
     2161 atccctcgca cggtgcgcca cgacgagttg cccgccgcgg tgtctgtgtc gtctctcggc
     2221 gtgcaagtgg ggctgctcgg cggaccggcg ctgggcggca tactcgtcga atccgtcggc
     2281 gccgcagcgg catacgcgat cgacgtcgcc ggtctcatca tcgcgaccgc gctcttcgcg
     2341 cggctcggtt cctatccccc cagcgaggac gcgaccccgg ccagcctgcg gtcgatcgcc
     2401 gagggcgcga ggtatgccgt gtcccgccgc gacctcctcg gcacctacgt catcgacatc
     2461 gtcgcgatgt tcatggcgat gccgatggtg ctcttccctg cgctggccaa cgacgtcctg
     2521 aagtcgccgc acacgctcgg gctgctctat tcggcgggca ccgtcggcag catgctggtc
     2581 acagcgactt ccggatggac gagccgggtc caccggcacg ggatggccgt tgtggtgtcc
     2641 gcagcagttt ggggcgcggc ggtcgcggcg gccgggctca cgacgaacgt gccaatcgtg
     2701 ttgctgtgct tcgcgattgc gggcgccgcc gacatggtga gcgcgctgtt ccggggcgtc
     2761 atctggcacc agacgatccc cgactcgatg cgcgggcgac tggccggcat cgagatgctg
     2821 tcctactcgg tcggcccgct cggcgggcag gtgcgctccg gactggtcgc cgaccgctgg
     2881 tcggtacgcg cgtcgatcac ctcaggtggg gtgctgtgcg tcatcggcgt ggtggcgacg
     2941 gccgcctggc tgcgcgactt ctggtcctac gacgcccgca ccgatccgta tgccgctgcc
     3001 gagcgccaac gccgtgccgc cgacggccgc gactgacgta gttcgggtgg caggatgagt
     3061 gagcaccgtc cggcagcttg aaggggtccg accatggcca gtgacgcagc ccgcaccacc
     3121 ggccgcaccg agcgcctgac gcagttccag cgcgatgggc tgaccttcga cgtcaccgac
     3181 tcggggcctc tcgacggccc ggtcgtcgtg ctcctgcacg gcttccccac cgatcgctcc
     3241 agctgggacc ggatctcgcc gctgctgcat gcggccggcc tgcgcaccct ggcacccgac
     3301 cagcgcggct actcgccggg agcgcggccg caggggcggg cggcataccg gctgggcgag
     3361 gtggccgacg acgtcgtggc gctggcggac gcggtcggag ccgacgcggt ccacgtcgtc
     3421 gggcacgact ggggcggagc catcgcctgg atcgtcgcgg ggcgccaccc cgagcgggtg
     3481 gcctcgctga ccgcgctgac cacaccacac cccttcgcga tgaccgaggc gctgcggtcg
     3541 cgcgacttcg accaggcgcg ccgctcctgg cacatgctcg cgttccaggt ccccgtgatc
     3601 cctgaccgct acctcgcgtc ccggctcgcg gcggtccttc ggcccagcgg gctgccggcg
     3661 gaggatgcga cccggtatgc cgcccgcttc tccgagccgg gcgccctcac ggccgcgctc
     3721 aactggtacc gcgccatccc cctctcctcc ggtgtcgtgc actcctgccg ggtgccgacg
     3781 acctatgtct gggggcgcaa ggatccgttc ctcggtccgg tggccgcccg gttgacggcg
     3841 cattcggtgc tggccgacta cgagttggtc gagctggatg agggtcactg gctgccggag
     3901 cgagcgccgc aggcctgcgc tcgcgccatc atcgcccggg tcgcctccgt cgactgaccc
     3961 accgcccccg ggtttccccc aaaatcctca cactcgccgg cactttctgg ctccccgagc
     4021 cctcgctgcc ccaagatccc cacacccgcc gatgacctgg gcgaggggta tgccggtcgc
     4081 ccccaagatc cccacacccg ccgatgatct gcgccgcccg gccccgcccg gccgcgaaac
     4141 cccgcctaga tttggcggcg tggacttcga ctcgctgccc gacctgatcc cggacgacac
     4201 cgcctattac cgccggatca gcgccgacac cttcctcccc acgctcaaca cccaaggcgc
     4261 ttggaatgcc cacgagcaac acatggcccc cgtctccggc ctcctcgccc acgccctgac
     4321 gacacacgag ccacgccccg agctagctct cgcccggatc acctacgaga tcctcggcct
     4381 catccccgcg atccccacga ccatcgaggt ccgcacgatc cgccccggcc gcaccatcga
     4441 gctcatcgag gcgaccgcga gcaccggcgg tcgaccggtc gtccgcgcca cagcctggcg
     4501 cctgtcccgt caggacacca ccgaggtcgc gggcggcgaa ccgtcacccc tgcccagccc
     4561 cgacgactgt gtcccgtggc ggccgtcgga gatctggccc ggcggctaca tcgcgagcat
     4621 cgagttgcgg catacccctg acaagcaacc cggcaccggg cgagcctgga tccgcaccga
     4681 caaagccgtg gtcgcggacg aggaggtggc gccgatcgcg gcatacctcg ggctcgtcga
     4741 caccgccaac gggatgaacg tccgcgtcga cccgagcgag tggctcttcc ccaacatcga
     4801 cctcaccgtc cacctccacc gggacccgct cggcggcccg gggcactggg tgggcttcga
     4861 cacgcaggtg accttcgggc gcgagggcgt cggcctcacc tcgacgacgc tgcacgacga
     4921 gagcggcgcg gtcggtcgag ccgagcagat cctcaccctc cgcgccgggc gctgaccagc
     4981 cagccggtat gccgtccgcg cccccgcatc cgctcctccc ctggatcgag gggtatgccg
     5041 tccgcctcgg ttcccctctc acctcaccga ccgcttgggt caccaccgca agcggcgccg
     5101 aattggcctt cgtcggcggt ctggtcgtca aactgcatca cccccgcacc gatgtcgccg
     5161 agctgcggac gcgactggcg ctggccactg atccggcgct tgcggcatac ctcgtgctgc
     5221 cggtaaccag cgaggtcgtg gtggcgccgg acggtcgagc ggtcaccgcg tggcctcggg
     5281 tagaggtcct cgaccccgcc gacgccgaca gtgaaggggc cacggcgccc tggaccgatg
     5341 ccggccggct gctggccggg ctgcaccggg caaggccgtt ggggtcgatg ccggtgcatg
     5401 ggggctgggc gcgactacgg cgcgcggtcg tgcgcgccga gggcggggct gacgtcgacc
     5461 cgaggctggg gatggtcgcg gcggccggtc agcgcgtcct cgccgctctt gatctggctg
     5521 cggccgacgc tgccccgcgg cccgaccggc acctcactgt ggtccacggc gactggcatc
     5581 tgggccaact cggtcgcgtc gacggggcgt ggcggctgct ggacatcgat gatctcgggt
     5641 ggggcgaccc ggcgtgggac ctggcccgac cggccgggtt ctgggctgct gggctgctgc
     5701 cgacggacga ttgggagggt ttcctcgcgg cataccgtgc tgccggcggt cccgg
//
LOCUS       DAOMZY010000240         5727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_13271_length_5727_cov_5.770451, whole genome shotgun sequence.
ACCESSION   DAOMZY010000240 DAOMZY010000000
VERSION     DAOMZY010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13271_length_5727_cov_5.770451"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..861
                     /gene="nucS"
                     /locus_tag="PLK64_12530"
     CDS             166..861
                     /gene="nucS"
                     /locus_tag="PLK64_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HPV80620.1"
                     /translation="MRLVIARCAVDYEGRLQAHLPIATRLLLVKADGSVLVHSDGGSY
                     KPLNWMAPPCSMAESEPDEGEREAGVVAVWTVRHTKSGDTLYVRIHEVLHDSSHELGA
                     DPGLIKEGVESHLQALLAEHIHTLGAGWSLVRREHMTAIGPVDILARDANRQHVAIEI
                     KRRGEIDGVEQLTRYLELLNRDPLLAPVAGIFAAQEIKPQARTLAQDRGIRCVVLDYE
                     ALRGIDDTESRLF"
     gene            complement(866..1204)
                     /locus_tag="PLK64_12535"
     CDS             complement(866..1204)
                     /locus_tag="PLK64_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80621.1"
                     /translation="MSDLQCPARVFLARDDLPGARVEAAVEGEHIAGRYAVPPGGPAD
                     CTWLDGIADRHRGEAVIVVAPVEVITSWLLGAGRHVKPADVLSFEIDSDGWRARAVVT
                     GPIPIQRPSH"
     gene            complement(1304..3409)
                     /locus_tag="PLK64_12540"
     CDS             complement(1304..3409)
                     /locus_tag="PLK64_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HPV80622.1"
                     /translation="MAETPSAAARPQGEPDAPARPTRERPWVIRTYAGHSSAAASNAL
                     YRRNLEKGQTGLSVAFDLPTQTGYDPDHVLSRGEVGKVGVPISHIGDMSALFEGIPLK
                     AMNTSMTINATAMWMLAMYQVTAEAQAEAAGEDPSAWVAALTGTTQNDIIKEYLSRGT
                     YAFPPAPSLRLITDMIAYTVSQIPKWNPTNICSYHLQEAGATPVQEIAYSMCTAIAVL
                     DAVRDSGQVPPERFGEVVARISFFVNAGVRFVEEMCKMRAFVQLWDEITQERYAVSDP
                     VARRFRYGVQVNSLGLTEAQPENNVQRIVLEMLAVTLSKDARARAVQLPAWNEALGLP
                     RPWDQQWSLRIQQVLAFESDLLEYDDLFNGSVVVAAKVRELVEGARAEIARVQAMGGA
                     VAAVESGYMKSALVASHSLRRQRIEAGEDIVVGLNQFTSTEPNPLTADLDTAIQTVDP
                     AVEQAAVAAIRAWRHTRDSDPAAKARADAALARLKADAASGVNLMPATLECARAKLTT
                     GEWAEALRGEFGEYRAPTGVSGSVGVSSSATPGGDLARVRADVARTGEELGEKLRVLV
                     GKPGLDGHSNGAEQIAVRARDAGFEVIYQGIRLTPEQIVAAAVAEDVHLVGLSILSGS
                     HLELVPDVLVGLREAGAGEVPVVVGGIIPESDAATLRAQGVAAVFTPKDFGLNDIMGR
                     FVEIIRESRGLAPLVSEPA"
     gene            complement(3450..3782)
                     /locus_tag="PLK64_12545"
     CDS             complement(3450..3782)
                     /locus_tag="PLK64_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPV80623.1"
                     /translation="MEVSELAAGVRVRVGGRLDAHSVAHVREVLHGVIDAGTGPVLIE
                     LPDAEIGDATALGLLVGAHHRARRLGRELYVGEVSERTARLLRMSHLDRVLAQAPEAM
                     RTVAALTA"
     gene            4037..4669
                     /locus_tag="PLK64_12550"
     CDS             4037..4669
                     /locus_tag="PLK64_12550"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPV80624.1"
                     /translation="MVNLTRIYTRTGDDGTTALGDFSRTPKTDPRLAAYADTNEANAC
                     LGVAVAAGGLREDLRTTLVRIQNELFDVGADLCLPLRETYDYPPLRVQQPWIDDLEAD
                     CDRYLAELQPLRSFILPGGSAGAAYLHVATTVVRRAERATWAAIEHYGTDLGVERGQG
                     GVNPLTATYLNRLSDLLFVLARVANLPDGDILWQPGGGRVEAPKPRGSRD"
     gene            complement(4734..5171)
                     /locus_tag="PLK64_12555"
     CDS             complement(4734..5171)
                     /locus_tag="PLK64_12555"
                     /inference="COORDINATES: protein motif:HMM:NF022199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2550 family protein"
                     /protein_id="HPV80625.1"
                     /translation="MPDLIFSVEVVVGIVVAVVVGYFLAVILRRRAISRGRLLAMCAY
                     AREGTHGWRNGFIRFGEGDVEWYPMGGISVRPKHAWSRRSLDLGTPVPVGRDKGLDFI
                     DDGVSVLCHDGTGAFRLGMARSAYTALRSFVESAPPESASSVN"
     gene            complement(5184..5447)
                     /locus_tag="PLK64_12560"
     CDS             complement(5184..5447)
                     /locus_tag="PLK64_12560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HPV80626.1"
                     /translation="MSELQVELVAADRRVWVGAAKMVRCRTSSGELGVMPGHTPMLGI
                     LVNGELIIHQADGAKQAATIDSGFLSVEHDRVTIVADHVDVQG"
     gene            complement(5494..>5727)
                     /locus_tag="PLK64_12565"
     CDS             complement(5494..>5727)
                     /locus_tag="PLK64_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80627.1"
                     /translation="AHLGRYGDGGAHLHVFLFGRPARAGQFRGSPLLDWEENLPKIPV
                     TVAAENARFVADRIVATYGGRAHTPAAGGPEDR"
BASE COUNT          930 a         1984 c         1971 g          842 t
ORIGIN      
        1 cacccgccgc gccaggcgac gccccgccct ccccaaaaga tcagcgcatc cctagcgcgt
       61 cagtatgccg ccaagcccag gggccgaccc cgggacgtta gtcatagggt gaccgagttt
      121 ggctgcgact ggtagccgaa cggttccccg ctaggctcac cggtcgtgcg tcttgtcatc
      181 gcccgatgcg ccgtcgacta tgagggacgg ctgcaggccc acctgcccat tgccacccgg
      241 ctgctgctgg tcaaggccga cggttcggtg ctcgtgcaca gtgacggcgg ctcctacaag
      301 ccgctcaact ggatggcccc gccctgctcg atggccgagt cggagcccga cgagggcgag
      361 cgagaggcgg gggtcgtcgc ggtctggacc gtgcggcata ccaagtctgg cgacaccctc
      421 tatgtccgga tccacgaggt attgcacgac tcctcgcacg agctcggagc cgaccccggg
      481 ctgatcaagg agggcgtgga gtcgcacctg caggccttgc tcgccgagca catccacacg
      541 ctgggggcgg gatggtcgct ggtgcgccgc gagcacatga cggcgatcgg ccccgtcgac
      601 atcctggccc gcgacgccaa tcggcagcat gtggcgatcg agatcaagcg ccgcggcgag
      661 atcgacggcg tcgagcaact cacccggtac ctcgagctgc tcaatcgcga tccgctgttg
      721 gcgccggtcg cggggatctt cgccgcacag gagatcaagc cacaggcgcg cacgctggca
      781 caggaccgtg gcatccggtg cgtcgtgctc gactatgaag cgctgcgcgg catcgacgac
      841 accgagtcac gcctgttctg aacggtcagt ggctcggacg ctggataggg atcggtccgg
      901 tgacgacggc ccgagcccgc cacccgtcag agtcgatttc gaaggacagg acgtccgccg
      961 gcttgacgtg tcgaccagca ccgagcagcc aggaggtgat gacctcgacg ggcgccacca
     1021 cgatcaccgc ctcgcctcgg tggcggtcgg cgatgccgtc cagccaggtg cagtctgccg
     1081 gtccgccggg agggacggca taccgaccgg cgatatgttc gccttcgacg gctgcctcga
     1141 cgcgcgcgcc aggcagatcg tcgcgagcga ggaacacgcg tgcaggacac tgcagatcgc
     1201 tcataccatc cagtatgaac acctcaggcc cgagcgcgag gaggagagcg gttgcattct
     1261 gcctgacgcg actcgcccag cggccctcgc gaagggcctg gacctaggcc ggctcggaaa
     1321 ccagcggggc cagcccccgc gactcgcgga tgatctcgac gaaccgaccc atgatgtcgt
     1381 tgagcccgaa gtccttgggg gtgaagacgg ccgcgactcc ctgggcccgc agggtggccg
     1441 cgtccgactc ggggatgatc cccccgacga cgacgggcac ctcgccggca ccggcctcgc
     1501 gcagcccgac gagcacgtcg gggacaagct ccaggtgcga cccggaaagg atcgacagac
     1561 ccacgaggtg cacgtcctcg gcgaccgcgg ccgcgacgat ctgctcgggc gtcagtcgga
     1621 tgccctgata gatcacctcg aagccggcgt cccgggcccg caccgcgatc tgctcggcac
     1681 cgttgctgtg accgtccagg ccgggcttgc cgacgagcac ccggagcttc tcgccaagct
     1741 cctcacccgt acgggcgaca tcggcccgga cccgggccag gtcgccgcct ggcgtggcgc
     1801 tggaggagac cccgaccgat ccggaaacgc cggtgggcgc gcggtattcg ccgaactccc
     1861 cgcgcaacgc ctcggcccat tcgccggtcg tcagcttcgc ccgggcgcac tccagagtgg
     1921 cgggcatgag gttgacccca ctggccgcgt cggccttgag ccgggccaga gccgcgtcag
     1981 cgcgcgcctt cgcggcgggg tcgctgtcgc gggtatgccg ccacgcccgg atcgcggcga
     2041 cggccgcctg ctcgacggcc gggtcgacgg tctggatggc ggtgtcgagg tctgcagtca
     2101 gcgggttggg ctcggtcgag gtgaactggt tgagcccgac gacgatgtcc tccccggcct
     2161 cgatccgctg gcggcgcagg gagtgcgacg cgaccagggc ggacttcata tagccggact
     2221 ccacggcagc gacggccccg cccatggcct ggacccgggc gatctcggcc cgggcaccct
     2281 cgacgagctc acggaccttg gcggcgacga cgaccgaccc gttgaacagg tcgtcgtatt
     2341 ccaacaggtc gctttcgaag gccaacacct gctggatgcg cagcgaccac tgctggtccc
     2401 acggccgcgg taggccgagg gcttcattcc aggccggcaa ctggacggcg cgggcgcggg
     2461 cgtccttgga cagcgtcacg gccagcatct ccaggacgat ccgctggacg ttgttctccg
     2521 gctgagcctc agtgagcccg agagagttga cctgcacccc gtaacggaac cggcgcgcga
     2581 ccgggtcgga cacggcatac cgctcctggg tgatctcgtc ccacaactgg acgaacgcac
     2641 gcatcttgca catctcttcg acgaagcgca cgccagcgtt gacgaagaac gagatccgag
     2701 cgacgacctc accgaagcgt tccggcggca cctgaccgga gtcgcgcacc gcgtccagga
     2761 cggcgatcgc cgtgcacatg gagtaggcga tctcctgcac cggcgtcgca ccggcctctt
     2821 gcaggtggta tgagcagatg ttcgtcgggt tccacttggg gatctgcgag accgtgtagg
     2881 cgatcatgtc ggtgatcagc cgcagcgacg gcgcgggcgg gaacgcatac gtcccccgcg
     2941 agaggtattc cttgatgatg tcgttctgag tggtcccggt gagggccgcc acccacgcgc
     3001 tcgggtcctc gccggcggcc tcggcctggg cctcggcggt cacttggtac atcgcgagca
     3061 tccacatggc cgtcgcgttg atcgtcatcg aggtgttcat ggccttgagc gggatgccct
     3121 cgaagagcgc cgacatgtcg ccgatgtggc tgatcggcac gccgaccttg ccgacctcgc
     3181 cccgggacag gacgtggtcg gggtcgtaac cggtctgggt gggcaggtcg aaggcgaccg
     3241 agaggccggt ctggcccttc tccagattgc gccggtagag ggcgttgctg gcggcggcac
     3301 tggagtgacc ggcatacgtg cggatcaccc aggggcgctc tctggtgggt cgagcgggcg
     3361 cgtcgggttc gccctggggg cgtgcggcag cggaaggtgt ctcggccatg gatctgacgc
     3421 taccggcagg tagccccacc tgcccagggt caggccgtga gtgctgccac agtgcgcatg
     3481 gcctccggcg cctgggcgag cacccgatcc aggtgcgaca tccgcagcag ccgggcggtg
     3541 cgctcggaca cctcaccgac gtagagctcg cggcccaacc tccgggcccg gtgatgcgct
     3601 ccgacgagca ggccgagggc cgtagcgtcg ccgatctcgg cgtccggcag ctcgatgagg
     3661 accgggccgg tgccggcatc gatcactccg tgcaggacct cgcgcacgtg ggccaccgag
     3721 tgtgcgtcca gtcggccgcc gacccgcact cgcacgcccg cagccagctc cgacacctcc
     3781 acggggacac cgcgaggaga cccggcatgg ctgtgtcgag tcggcatcgt gcggtcaccc
     3841 cctgtggcga cggaaagcat gtgtcggtgg cgtcggcgcg cccttcggat cgtcacgcta
     3901 gaggaggagc cgggtgagcg acgccagaga cacgctgtga cggatgttac gaaagagaca
     3961 caagtgtatg ccgtgacgtc tgggatctgc gagcgtccga cccgcgcgtc ggcccggatc
     4021 tgacaggctg ggcgtcatgg tcaacctcac ccgcatctac acccgcaccg gcgacgacgg
     4081 gacaaccgcg ctgggcgact tcagccggac cccgaagacc gacccgcggc tggcggcata
     4141 cgccgacacg aacgaggcca acgcctgcct gggcgtcgcg gtcgctgccg gaggcctgcg
     4201 ggaagacctg cggaccacgc tggtgcgcat tcagaacgag ttgttcgacg tcggcgccga
     4261 cctgtgtttg ccgttgcgcg agacctacga ctacccgccg ctgcgggtcc agcagccgtg
     4321 gatcgacgac ctggaagccg actgtgaccg ctatctggcc gagttgcagc cgctgcgctc
     4381 gttcatcctc cccggagggt cggcgggtgc ggcataccta cacgtcgcca cgaccgtggt
     4441 gcgccgggcc gagcgagcga cgtgggcggc catcgagcac tacggaaccg atctgggggt
     4501 cgagcgcggc cagggcggcg tcaaccccct gacggcgacc tacctcaacc ggctctctga
     4561 cctgctgttc gtgttggctc gagtggccaa cctgcccgac ggcgacatcc tctggcagcc
     4621 cggcggcggc cgagtggaag cgcccaagcc ccgcggctct cgcgactgag gggtccgact
     4681 cgctgatgag gcgacttgcc gacgaggcga ctcgctgact cggcggtgag cggtcagtta
     4741 accgacgacg ccgactccgg cggcgccgac tcgacgaacg aacgcaaggc cgtataggcc
     4801 gaccgagcca tgcccaggcg gaaggcgccc gtaccgtcgt ggcacaggac cgacacgccg
     4861 tcgtcgatga aatccagacc tttgtcgcgc cctacgggga ccggggtgcc gaggtcgagg
     4921 ctgcgccgtg accaggcgtg tttcgggcgc accgagatgc cgcccattgg ataccactcg
     4981 acgtcaccct cgccgaagcg gataaacccg ttgcgccacc cgtgggtccc ctcgcgggcg
     5041 taggcgcaca tggccaacag cctcccgcgc gagatggccc ggcggcgcag gatgacggcg
     5101 aggaagtagc cgacaacgac ggccacaaca atcccgacca cgacctcgac ggaaaagatc
     5161 aggtcgggca cgagcggctc cggtcagccc tgaacgtcga cgtggtcggc gacgatcgtc
     5221 actcggtcat gctcgacgga gaggaacccg ctgtcgatgg tggcggcctg ctttgcgccg
     5281 tcggcctggt ggatgatcaa ctcgccgttg acgaggatgc ccagcatcgg cgtgtggccg
     5341 ggcatgacgc cgagttcgcc ggacgaagtg cggcagcgca ccatcttggc cgccccgacc
     5401 cagacgcgcc ggtctgcggc gaccagctcg acctgcagtt cactcacgat gccctccgga
     5461 cgatcggctt cagccctcta ctgctgcccg agtctatcgg tcctccggac ctccagctgc
     5521 cggggtgtgc gcgcgcccac cgtaggtcgc cacgatccgg tcggcgacga acctcgcgtt
     5581 ctctgccgcc acggtaaccg ggatcttggg gaggttctcc tcccagtcca gcagcggtga
     5641 cccacggaac tgaccggccc gagcaggccg cccgaagagg aacacatgca ggtgggcgcc
     5701 cccgtcgcca tatcggccga ggtgggc
//
LOCUS       DAOMZY010000241         5683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_13386_length_5683_cov_6.684080, whole genome shotgun sequence.
ACCESSION   DAOMZY010000241 DAOMZY010000000
VERSION     DAOMZY010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13386_length_5683_cov_6.684080"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="PLK64_12570"
     CDS             <1..929
                     /locus_tag="PLK64_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-24"
                     /protein_id="HPV80628.1"
                     /translation="AAALTLRLVMGVSTADLARLFLVAEPTMAARLTRARKKLAVAGV
                     AFALPTPERLAERIDVVATVAYLAFTAGYAPGSGPDVVRAELAGEAIRLTRLLRQLLQ
                     GYAVQPPSAATQSAPLLDALLALMLLQHSRREARVDDAGRLVLLPDQDRGRWKRGEIA
                     DALGLLAGLPDDPAVLGRRAAEVRLQALIAAEHAVALRAGDTDWRRIVNRYAALDRLT
                     ASPVVRLNRAVAVAEADGPEAGLALLDGLDEVMPTSHRVSTVRAELLVRAGRAEEAAA
                     AYERAIGLCTNEAEHAHLRGRREALGREPGRP"
     gene            complement(926..1564)
                     /locus_tag="PLK64_12575"
     CDS             complement(926..1564)
                     /locus_tag="PLK64_12575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HPV80629.1"
                     /translation="MNQTSSPSAPAVVDPRLTTDPRPDFFAAADQAVHLIDGLAPADL
                     DRPTPCDDYDVRTLVAHLLTVFRRITHVATGGGWAEVPQVITGVPDESLATVAAADRD
                     RLVQVWSDDAVLERILSLPPGVQVPGRIGAFRYTQELVTHAWDLAAAVGRVDQLDPAL
                     AEPVIIGAQQFVPRDAREGFPFGAVVDVADDAGPYERLVGWLGRDPNWTPSA"
     gene            1626..2684
                     /locus_tag="PLK64_12580"
     CDS             1626..2684
                     /locus_tag="PLK64_12580"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HPV80630.1"
                     /translation="MRSDRLLSLLLILQARGSAVAPEVARELEVSVRTVYRDVEALSA
                     AGVPVWTEQGRGGGIRLMPGYRTDMTGLTAAESRALVAMSGRAVPDDLGMGSALASAV
                     HKLVAAVPPSHRSAAEAARARVLVDHAGWYRRTPSAPALEVVQEAVWAQRRLLLDYRH
                     GDGTEESVTVDPYGLVAKTGVWYLVGSTGTGEDHHERMWRVDRVNTAVALDEPATEPD
                     GLDLAAVWERLRQGIERPRGLVPVEVVVRIKPDVLDRVHRVTAARRFLAPDETDPTPP
                     GMPPAATDPAATHADRTTDSDGWIRMTLCFRGEMGAVAALLAFGGDAEIVHPPSARRL
                     MAQTARAALARHTGEEPA"
     gene            2822..3853
                     /gene="fbaA"
                     /locus_tag="PLK64_12585"
     CDS             2822..3853
                     /gene="fbaA"
                     /locus_tag="PLK64_12585"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPV80631.1"
                     /translation="MPIATPEVYADMLDRAKAGAFAYPAINITSSQTINAAIRGFAEA
                     GSDGIIQVSTGGADFASGSNIKNMVTGASALAAFAAEVAKNYSVNIALHTDHCPKDKL
                     DGFVRPLLAISAERVKAGLPPLFQSHMWDGSAVPLAENLTIAQDLLALAKAAHIILEV
                     EIGVVGGEEDGVANEINEQLYTTPEDALATVEALGLGENGRFMAALTFGNVHGVYKPG
                     NVKLRPEILKQCQDAVVAAKGLAAGSKPLDLVFHGGSGSLPQEISDAVDFGVIKMNVD
                     TDTQYAFTRPVAGWMLKNYDGVLKVDGEVGNKKQYDPRAWGKEAEAAMAARVVRACQD
                     LRSAGTHQA"
     gene            3856..4323
                     /locus_tag="PLK64_12590"
     CDS             3856..4323
                     /locus_tag="PLK64_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3151 domain-containing protein"
                     /protein_id="HPV80632.1"
                     /translation="MTSNLLGMPATLLPVDPAADIVAGLREGPGHGSPDHAGSAAEGM
                     PHAALESVAAAYPTSSLAWALLAEGALDADHPVTAYAYARTGYHRGLDSLRKAGWRGQ
                     GPIPWEHEPNRGFLRALAALARAARVIGESEEEARCAGFLRESSREAADALGL"
     gene            complement(4413..4808)
                     /locus_tag="PLK64_12595"
     CDS             complement(4413..4808)
                     /locus_tag="PLK64_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010524569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPV80633.1"
                     /translation="MIVDTSAVVAILLREPGSQALVDAILDAPSAKMSAGSALELQVV
                     ASRRGGDAGRVAALTILDDLSIDITAVTAQQALDAGAAHSRFGRGSGSKARLNFGDCF
                     AYALAAESDEPLLFVGDDFTHTDLTPVLP"
     gene            complement(4805..5086)
                     /locus_tag="PLK64_12600"
     CDS             complement(4805..5086)
                     /locus_tag="PLK64_12600"
                     /inference="COORDINATES: protein motif:HMM:NF019324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HPV80634.1"
                     /translation="MALNIKNERVCRLAKEAAERTGRSQVSVLEAALEDYLAKVSAAD
                     AAKQESRAARVARVHATVEAYNVGLTDEDRAAVWRFMNDEMYDEDGLPA"
     gene            complement(5154..>5683)
                     /locus_tag="PLK64_12605"
     CDS             complement(5154..>5683)
                     /locus_tag="PLK64_12605"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HPV80635.1"
                     /translation="LGLIPRDEEWLAGRALAGVWFATIEDSAAHQHSMDTAPYAGHAG
                     VVVSPLATGRLDPPDVVLIYATPGQMVLMINGLQWSGYRKLTMSSVGESACADSWGRA
                     LATGEPSVSIPCYAERRYGGVADDEMLMATPPQFLPTMIAGLEALSRNGLRYPIAPYG
                     IQADARAGLAKSYGN"
BASE COUNT          866 a         1983 c         1990 g          844 t
ORIGIN      
        1 ccgcagcggc gctgaccctc cggctggtga tgggcgtctc gacggctgac ctcgctcggc
       61 tgttcctcgt cgccgagccg acgatggcgg cccggttgac ccgggctcgc aagaagctcg
      121 ccgtcgcggg cgttgcgttc gcccttccga cgcctgagcg gctcgccgag cggatcgacg
      181 tggtcgccac ggtcgcctac ctcgccttca ccgccggtta tgcgcccggt tccggtcccg
      241 atgtcgtgcg cgccgaacta gcgggggaag cgatccggct cacccgcctg ctgcgccaac
      301 tcctccaggg gtatgccgtt cagccgcctt ccgctgcaac gcaatccgcg cccctgctcg
      361 atgcactgct ggcgttgatg ctgctgcagc actcgcggcg cgaggctcgg gtggacgatg
      421 ccggacggct ggtgttgctg cccgaccagg accgcggccg atggaagcgc ggcgagatcg
      481 ccgatgctct cggtctgctc gcggggttgc ccgacgaccc agcggtgctg ggtcgtcggg
      541 ctgcagaggt tcggctgcag gccctcatcg cggccgagca cgcggtcgcg ctcagggccg
      601 gcgacaccga ttggcgacgg atcgtcaacc ggtatgccgc cctcgaccgc ctcacggctt
      661 cgccggtggt ccggctcaac cgtgcggtgg ccgtggctga agccgacggc ccggaggctg
      721 gcctggcctt gcttgacggc ctcgacgagg tgatgcccac gtcgcatcga gtgtcgacgg
      781 tgcgggccga gttgctggtc cgggccgggc gcgcggagga ggccgcggcg gcatacgaac
      841 gggcaattgg cttgtgcacc aacgaagccg agcatgccca cctgcggggc cgccgcgagg
      901 cactcgggcg cgagccgggc cgaccctagg cggacggcgt ccagttcggg tcgcggccca
      961 gccagccgac cagccgctcg taagggccgg cgtcgtcggc cacgtcgacc accgccccga
     1021 aggggaagcc ctcacgggca tcgcgcggga cgaactgctg ggcgccgatg atgaccggct
     1081 cggcgagtgc cgggtcgagc tgatccacgc gaccgacggc cgcagcgagg tcccacgcgt
     1141 gggtgacgag ctcctgggtg tagcgaaagg ctccgatgcg tcccggcacc tgaacgcccg
     1201 gggggaggct gaggatccgc tctaggaccg cgtcgtcgga ccagacctgc acgagccggt
     1261 cgcggtcggc ggcggcaact gtcgcgagcg actcgtcggg gacgccggtg atgacctgtg
     1321 gcacctcggc ccagccgccg cccgtggcca cgtgggtgat ccggcggaag acggtcaaga
     1381 ggtgggcgac gagggtgcgc acgtcgtagt cgtcgcaggg agtcggtcgg tcgaggtcgg
     1441 cgggcgcgag gccgtcgatg aggtggaccg cctggtcggc tgcggcgaag aagtcgggac
     1501 gggggtcggt cgtcagacgc gggtcgacga cggcgggggc gctgggggaa gaagtttggt
     1561 tcatggctcc accctgagcc ctgaacctga cacctacggt caggtttctc tgccattctc
     1621 gatccatgcg atctgaccgg ctcctcagcc tgctgctcat cctgcaggcc cgcggcagcg
     1681 ccgtcgcccc cgaggtcgca cgcgagttgg aggtcagcgt ccgcaccgtc tatcgcgacg
     1741 tcgaggcact ctcggcggcc ggcgttccgg tgtggaccga gcagggtcga ggcgggggca
     1801 tccggctcat gcccggctat cgcaccgaca tgacaggctt gaccgcggcc gagtcgcggg
     1861 ccctggtggc gatgtccggt cgagccgtgc ccgacgatct gggcatgggg tcggccctgg
     1921 cgagcgcggt ccacaaactc gttgccgcag tgccgccttc gcatcgctcg gctgccgaag
     1981 ccgcccgcgc gagggtcctc gttgaccatg ctggctggta tcgccgcacc ccttccgcac
     2041 ccgcgctcga ggtcgttcag gaggccgtgt gggcgcagcg ccgactgctc ctggactatc
     2101 ggcacggcga cggcaccgag gagtcggtca ccgtcgaccc ctatggcctg gtcgccaaga
     2161 cgggagtctg gtacctcgtc ggcagcaccg gcaccggcga ggaccaccac gaacggatgt
     2221 ggcgcgtcga ccgggtcaac accgcagtcg ccctggacga gccggccacg gagccggacg
     2281 ggctcgacct cgcggcggtg tgggaacggc tgcgacaggg gatcgaacgg ccccgcgggt
     2341 tggtgccggt cgaggtcgtc gtgcggatca agccggacgt cctcgacagg gtgcatcggg
     2401 tgaccgccgc ccgcaggttc ctcgccccgg acgagaccga ccccacacca cccggtatgc
     2461 cgcccgcagc cactgacccc gcagccaccc acgctgacag gaccaccgac tccgacggct
     2521 ggatccgcat gaccctgtgc ttccgtggcg aaatgggcgc tgtcgcagca ctattggcgt
     2581 tcggcggcga cgccgagatt gtccatccgc cctcggcccg gcggctcatg gcgcagaccg
     2641 cgagagccgc gctggcacgg catacgggag aagaaccggc gtgaggccca gcgttggtga
     2701 ggtgaacgtg aggtgacgca gaggtagccg acgtgttcgg ggcgaacgtc cctgggcaag
     2761 cgcttgccag gaagtggaag gatgggggcg gaccgaccgc cgagcgccta ggagacgccc
     2821 catgcccatc gcaacacctg aggtttacgc cgacatgctc gaccgcgcga aggctggcgc
     2881 ctttgcctac ccggcgatca acatcacctc cagccagacc atcaacgcgg cgattcgtgg
     2941 cttcgccgag gccggctccg acggcatcat ccaggtgtcc accggtggcg ccgacttcgc
     3001 gtcgggctcc aacatcaaga acatggtgac cggcgccagc gccctcgccg cgttcgccgc
     3061 cgaggtcgcc aagaactact cggtcaacat cgcgctgcac accgaccact gcccgaagga
     3121 caagctcgac ggcttcgtcc gtccgctgct ggccatctcg gccgagcggg tcaaggccgg
     3181 cctgcccccg ctgttccagt cgcacatgtg ggacggctcg gccgtgccgc tggccgagaa
     3241 cctcaccatc gcccaggacc tgctggccct cgccaaggcc gcgcacatca tccttgaggt
     3301 cgagatcggt gtcgtcggcg gcgaagagga cggcgtcgcc aacgagatca acgagcagct
     3361 ctacacgacc cccgaggacg ccctcgcgac cgtcgaggcg ctgggcctgg gcgagaacgg
     3421 ccgcttcatg gctgccctca ccttcggcaa cgtgcacggc gtctacaagc cgggcaacgt
     3481 caagctgcgc cccgagatcc tcaagcagtg ccaggacgcg gtcgtcgcgg ccaagggcct
     3541 ggctgctggc agcaagccgc tggacctcgt cttccacggc ggctccggct cgctcccgca
     3601 ggagatctcg gacgcggtcg acttcggcgt catcaagatg aacgtcgaca ccgacacgca
     3661 gtacgccttc acccgcccgg tcgccggctg gatgctcaag aactacgacg gcgtcctcaa
     3721 ggtcgacggc gaggtcggca acaagaagca gtacgacccg cgcgcctggg gcaaggaggc
     3781 cgaggccgcc atggccgccc gcgtcgtccg cgcctgccag gacctgcgct cggccggaac
     3841 gcaccaggcc tgagggtgac ctcaaacctg ctgggtatgc cggcgacgct actgccggtc
     3901 gaccccgcgg ctgacatcgt ggccgggctc cgtgaggggc ccggccacgg gtcgccagac
     3961 catgcgggaa gcgccgcaga gggtatgccg cacgcggccc tcgaatccgt tgccgcggca
     4021 tacccgacct cgtcgttggc ctgggccctg ctggccgagg gggcgctcga cgcggaccac
     4081 ccggtcacgg catacgcgta tgcccgcacg ggttatcacc gcggtctgga ctcgctgcgc
     4141 aaggccgggt ggcgtggtca gggaccgatc ccgtgggagc acgaacccaa ccgggggttc
     4201 ctgcgggcgc tggcggcgct ggcccgagcg gcccgcgtca tcggggagtc cgaggaagag
     4261 gcccgctgcg ccggcttcct gcgcgagtca tcccgcgagg ctgccgacgc tctcggcctc
     4321 tgagctgttg ttcccctttg ccccccccgc cgggtcgagg tattccacca tcgccaggtg
     4381 atggccgaat acctcgaccc agccggggtg ggctagggga ggacaggcgt gaggtcggtg
     4441 tgggtgaaat cgtcgccgac gaacagcaac ggctcgtctg actccgcggc caacgcgtag
     4501 gcgaagcagt ccccgaagtt caggcgggcc ttgctccctg aaccccggcc gaatcggctg
     4561 tgggcggccc cggcatcgag ggcctgctgc gcggtcaccg cagtaatgtc gatcgacaag
     4621 tcgtcaagaa tcgtgagggc ggccacgcgg cctgcgtcgc ccccgcgtcg actggcgacg
     4681 acctgcaact ccagagccga ccccgccgac atcttggcgc tgggggcgtc aagaatggca
     4741 tccaccaggg cttgcgagcc cggctcgcgc aacaggatgg cgacgacagc cgaggtgtcg
     4801 acgatcatgc gggcaacccg tcttcgtcgt acatctcatc attcatgaag cgccagacag
     4861 ctgcccggtc ctcgtcggtg agcccgacgt tgtaggcctc gacggttgcg tgcacccggg
     4921 ccacgcgtgc cgctcggctc tcttgcttgg cggcatcggc ggccgacacc ttcgccaaat
     4981 agtcttcgag agcagcctcc aggacgctga cctggctgcg gcccgtgcgc tcggcggcct
     5041 ccttggccag gcggcatacc cgctcgttct tgatgttgag cgccatgctt acaccttaca
     5101 cctgcatatg ttacaccttg agcccgaaca gccctcgaag gtgggctcgc gggttagttt
     5161 ccgtagctct tggccagccc cgcgcgggca tcggcctgga tgccgtaggg cgcgatcggg
     5221 tagcgaagac cgttgcggga caacgcttcc aggcccgcga tcatcgtcgg cagaaactgc
     5281 ggcggcgtcg ccatgagcat ctcgtcgtca gcaaccccgc catagcggcg ctcggcataa
     5341 caggggatcg acaccgacgg ctcgccggtg gccagcgccc ggccccagga gtcggcgcag
     5401 gccgactcgc cgaccgagct catggtgagt ttgcggtagc ccgaccactg cagcccgttg
     5461 atcatgagca ccatctgacc tggcgtggca tagatgagca cgacgtcggg tggatccagt
     5521 cgccccgtcg cgaggggaga gacgacgaca ccggcgtggc cggcatacgg ggcggtgtcc
     5581 atcgagtgct ggtgcgccgc ggagtcctcg atcgtcgcga accagacccc ggccagcgcg
     5641 cgtccggcca gccactcctc gtcgcgcggg atcaggccaa gca
//
LOCUS       DAOMZY010000242         5656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_13465_length_5656_cov_5.053383, whole genome shotgun sequence.
ACCESSION   DAOMZY010000242 DAOMZY010000000
VERSION     DAOMZY010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5656
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13465_length_5656_cov_5.053383"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PLK64_12610"
     CDS             complement(<1..536)
                     /locus_tag="PLK64_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPV80636.1"
                     /translation="MSSLFLRTLREDPADAEVPSHRLLVRAGYIRRVSPGIYTWLPLG
                     LRVLRNVEQIVREEMDQISQELLFPALLPREPFDATGRWVEYGDNIFRLKDRKGGDYL
                     LGPTHEELFTLAVKDVFSSYKDLPISLYQIQTKYRDEARPRAGLLRGREFVMKDSYSF
                     DVDEAGLAKSYDAHRDAY"
     gene            697..942
                     /locus_tag="PLK64_12615"
     CDS             697..942
                     /locus_tag="PLK64_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HPV80637.1"
                     /translation="MSITASEARRNLFPLIEQVNEDHTPIEISSRRGNAVLMSRADFD
                     ALEATAYLLRVPANAHWLLDSLAQARQGQTVEHDLAR"
     gene            939..1196
                     /locus_tag="PLK64_12620"
     CDS             939..1196
                     /locus_tag="PLK64_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008732381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HPV80638.1"
                     /translation="MRLVFTANGWADYTHWQTADRAMLRRINRLIDESLRDPTAGIGK
                     PERLRHLLAGAWSRRINDEHRLVYLVDGEDLVILTARFHYE"
     gene            complement(1213..2688)
                     /locus_tag="PLK64_12625"
     CDS             complement(1213..2688)
                     /locus_tag="PLK64_12625"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HPV80639.1"
                     /translation="MRSPRTRRRYAARLTALTVAALVLITGCTVGAGTGVGAPSTRSE
                     LPRDTIVNLFQWNWRSVGAECPRLAKVGYAGVQVSPAQEHAQVVNNPWWVDYQPVSYQ
                     IASRRGDRAAYQGMVAACHAVGVKIYADAVINHMAGGDAGTGWAGSAWTHYDYPGIYQ
                     NQDFHHCGIAANDDISDYQNRAQVQTCELVNLADLDTGSDYVRGRIAAYLNDLISLGV
                     DGFRVDAVKHLSADDMAAIVGRLSTPVERYYEVIDKGGEPISMTEYTGSGKVLVFSYG
                     DEVGSALQWGKISALRNLAGGVDGTVARVFLDNHDTQRHGGAQVLTYKQPRLYALANV
                     FMLAWPYGEPTVMSSYAFTDPDAGPPSTDQIGTTKDTVCGSDGWECEHRWQSTEGMVG
                     FHNVVKGTGVDLWWEDPSGNSIAFARDDRGYVVINNATSPLAGRSFQSRMPPGTYCDV
                     VHGALVNGTCTGPTVTVKADGWFTADVGNLDALAIHVGAKQ"
     gene            2898..4325
                     /locus_tag="PLK64_12630"
     CDS             2898..4325
                     /locus_tag="PLK64_12630"
                     /inference="COORDINATES: protein motif:HMM:NF017591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HPV80640.1"
                     /translation="MLMRSRLITTAACLALAPTLFASTALAAPGSTRGPSSSQSPYLL
                     PVAPGVMNTALLSVGDAADNGYRMVGIPDGLGAFDNGDGTITVLMNHELRSDRGIVRA
                     RGAKGAFVSKWIIEKKTLRILEGSDLIKQVVLVNGDSAIARLCSADLPPISAFYNADS
                     GKGYNGRIFMDGEEDGSDAKGRGFAHVVETGISYDLPSLGRFAYENALAHPNTGDATV
                     VVGTDDSTPGQVYVYAGQKRTEGTPVEKAGLVGGALYGIKVAGGLDEMNNPEFPLSST
                     FSLVPLAGANLTVSQLQAASEAAGVTEFWRPEDGHWDTKNPNVFWFATTANFDGPTRL
                     WKVTFADAAKPELGGTIEAALAGTEGLHMLDNLTVADKGGQLLLQEDPGNQEYLARVY
                     SYNSRTDRVTPILEHDPARFSTGGSQFLTTDEESSGVIDVSSLLGEGSYLLTTQVHKA
                     SDDAELVELGQLQLVRVAWKDNGKG"
     gene            complement(4426..5274)
                     /locus_tag="PLK64_12635"
     CDS             complement(4426..5274)
                     /locus_tag="PLK64_12635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV80641.1"
                     /translation="MLRTHTPVRALTPADRDEALALCARDPAANCFVAGRIEEGALHS
                     MPGALLGVPGQDGDLRSVAWASANLVPVECDLPSLDAIAAKVRRWRRHCASIFGPVDQ
                     VAGLWERLGPAWGPARTIRREQLLMSTGVDPGDWGVAIDPRVRLARMDELDLVVPAAA
                     AMFTEEIGYPPFVGSSANYRAVVSGLIRSGRTYVWVDQGRVLFKADVGSVGVDAAQIQ
                     GVWLAPALRGQGLATSLMAAVTSAVLRGIASEATLYVNDFNTPAIATYEAVGFRRVGT
                     FATILL"
     gene            complement(5302..>5656)
                     /locus_tag="PLK64_12640"
     CDS             complement(5302..>5656)
                     /locus_tag="PLK64_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80642.1"
                     /translation="LVALVGVGIFLGAGAVFLPSTLVGGAVLSHRRIPTWARVAAVVA
                     VLGWGVAMVRFASADNDPLGLYVATPRFLAGAGLMAGVGLAMAWGSSRVFAPWAPIRG
                     ISALPGDAGVPRIGR"
BASE COUNT          940 a         1966 c         1887 g          863 t
ORIGIN      
        1 atgtaggcgt cgcggtgggc gtcgtaggac ttggccagac cggcctcgtc gacgtcgaag
       61 gagtaggagt ccttcatgac gaactcgcgc ccgcgcagca ggccggcgcg cggccgggcc
      121 tcgtcgcggt acttcgtctg gatctgatag aggctgatcg gcaggtcctt gtaggaactg
      181 aagacgtcct tgaccgcgag ggtgaagagc tcctcgtggg tcgggccgag caggtagtcc
      241 ccgcccttgc gatccttgag ccggaagatg ttgtcgccgt actccaccca ccggccggtc
      301 gcgtcgaagg gctcgcgagg cagcagcgcc gggaacaaca gctcctggct gatctggtcc
      361 atctcctcgc gcacgatctg ctcgacgttg cgcaggaccc gcaagcccag gggcagccag
      421 gtgtagatgc ccgggctcac ccggcggatg tacccggccc gcacgagcag ccggtggctg
      481 ggcacctcgg cgtcggccgg gtcctcgcgg agggttcgca ggaacagcga ggacatgcgc
      541 aggacggtca cggtctctcc ttagggggtg ccccggcagg atatcggccc gtatgccgcc
      601 cgctcaccgc cgtttcgcgg gccctgaacg tgcgctggct ggcccggcga ggcaaagtgg
      661 taccatgagc ggtaccacag ccgccgggga gttcacatgt cgatcacagc cagcgaggcg
      721 cgtcggaacc tctttccgct catcgagcag gtcaatgagg accacacgcc catcgagatc
      781 tcctcgcgcc gcggcaatgc cgtgctcatg tcgcgagcgg actttgacgc gttggaggcc
      841 acggcatacc tcctgcgggt gcctgccaac gcgcactggc tgctcgacag cctggcccag
      901 gcccgccaag gccagacggt cgagcacgac ctcgctcgat gaggctggtc ttcaccgcca
      961 acgggtgggc tgactacacc cattggcaga ccgccgaccg ggccatgctc aggcggatca
     1021 accggctgat cgatgagtca ctgcgcgacc caaccgcggg aatcggcaaa cctgagcggc
     1081 tgcggcacct gcttgccggg gcgtggtccc gacggatcaa cgacgaacac cggttggtct
     1141 acctcgtgga cggcgaggac ctggtcatcc tgaccgcccg cttccattac gaatagcgga
     1201 tgtggacctc ggtcactgct tggcgccgac gtggatggcc agggcgtcga ggttgccgac
     1261 gtcggcggtg aaccagccgt cggccttgac cgtcaccgtc ggcccggtgc acgtcccgtt
     1321 caccagcgcg ccgtgcacga cgtcgcaata ggtgccgggc ggcatacgcg attggaagga
     1381 tcgaccggcg agcggtgagg tcgcgttgtt gatgacgacg tagccgcggt cgtcgcgcgc
     1441 gaaggcgatc gagttgccgc tcgggtcctc ccaccacagg tccacgccgg tgcccttgac
     1501 gacgttgtgg aagccgacca tgccctcggt cgactgccag cggtgctcgc actcccagcc
     1561 gtccgagccg cagaccgtgt ccttcgtcgt gccgatctga tcggtcgacg gcggccccgc
     1621 gtcggggtcg gtgaaggcat acgacgacat gaccgtgggc tcgccgtagg gccaggccaa
     1681 catgaacacg ttggccagcg catagagccg cggctgcttg taggtgagca cctgcgcgcc
     1741 accgtgccgc tgcgtgtcat ggttgtcgag gaacacccgc gccaccgtgc cgtctactcc
     1801 cccagcgaga ttgcgcagcg ccgagatctt gccccattgg agcgccgacc ccacctcgtc
     1861 gccgtagctg aagacgagca ccttgcccga ccccgtgtat tcggtcatcg agatcggctc
     1921 gccgcccttg tcgatcacct cgtagtagcg ctccaccggc gtgctgagac gccccacgat
     1981 cgcggccatg tcgtcggccg acaggtgctt gacggcgtcg accctgaagc cgtcgactcc
     2041 cagcgagatg aggtcgttga ggtatgccgc gatccgcccc cgcacatagt ccgacccggt
     2101 gtcgaggtcg gcgaggttga ccaactcgca ggtctgcacc tgggcacggt tctggtagtc
     2161 ggagatgtcg tcgttggctg cgatcccgca gtggtggaag tcctggttct ggtagatccc
     2221 cgggtagtcg taatgcgtcc acgccgagcc cgcccaccca gtgcccgcgt cgccgccggc
     2281 catgtggttg atgaccgcat cggcatagat cttcaccccc accgcgtggc aggcggccac
     2341 catgccctgg tatgccgctc ggtcacctct gcggctggcg atctggtagc tcaccggttg
     2401 gtagtccacc caccacgggt tgttgacgac ctgggcatgc tcctgggcgg gcgagacttg
     2461 gaccccggca tacccgacct tggccagcct tgggcactcc gcgccgaccg agcgccagtt
     2521 ccattggaag aggttgacga tggtgtcgcg aggcagttcg gagcgggtgc tgggcgctcc
     2581 gacgccggtg cccgcgccca ccgtgcagcc ggtgatcagc accagagccg ccacagtcag
     2641 cgcggtgagg cgggcggcat acctgcgtcg ggtgcgggga gagcgcatgc tggcacgcta
     2701 cctcgcgact ttgccttgcg ctcggccgag gcaccgcgcc agcgccgggt cagcgctggg
     2761 tcccgactct gtgcgggcca ccgtcggcgg tttctcaggt ttcacccgat gctcgccttc
     2821 ccttcggtcg ccggtccccg tcccggcacg tgggctcggt tggttagtgg cacacccgta
     2881 accctggaag gaaccacatg ttgatgcgtt cgcgcctcat caccacggcc gcctgcctgg
     2941 ccctggcacc caccctgttc gctagcaccg cgctggcagc gcctggctcg acgagaggcc
     3001 cgagctcaag tcagtccccc tacctcctcc ccgtcgcccc cggcgtcatg aacacggccc
     3061 tgctgtcggt cggtgacgcg gcggacaacg gctaccggat ggtgggcatc cccgacggcc
     3121 tcggcgcgtt cgacaacggc gacggcacca tcaccgtgct gatgaaccac gaactgcgct
     3181 ccgaccgcgg catcgtccgg gcccgcggcg ccaagggcgc gttcgtgtcg aagtggatca
     3241 tcgagaagaa gacgctccgg atcctggaag gcagcgacct gatcaagcag gtcgtcctgg
     3301 tcaacggcga ctcggcgatc gcccgactgt gctccgccga cctgcccccg atctcggcgt
     3361 tctacaacgc ggattccggt aagggctaca acggccggat cttcatggac ggcgaagagg
     3421 acggctcgga cgccaagggc cgcgggttcg cgcacgtggt cgagaccggg atctcctacg
     3481 acttgccgtc gctcggtcgc ttcgcgtatg agaatgcgct ggcccacccc aacaccgggg
     3541 acgccacggt cgttgtcggc accgacgatt ccacacctgg ccaggtctac gtctacgcag
     3601 gccagaagcg caccgagggc acgcccgtcg agaaggcggg cctcgtcggc ggcgccctct
     3661 atggcatcaa ggtcgccggc ggcctagacg agatgaacaa ccccgagttc ccgctgagca
     3721 gcaccttctc gttggtgccg ctggcgggcg ccaacctgac cgtgtcgcag ttgcaggccg
     3781 cgtccgaggc cgctggggtg accgagttct ggcgccccga ggacggccac tgggacacga
     3841 agaaccccaa cgtgttctgg ttcgccacga cggcgaactt cgacgggccg acccggctgt
     3901 ggaaggtcac gttcgccgac gccgccaagc ccgagttggg tggcaccatc gaggccgcat
     3961 tggccggcac cgagggtctg cacatgctcg acaacctgac ggtggccgac aagggcgggc
     4021 agctgctcct gcaggaggac ccgggcaacc aggagtacct ggcgcgcgtc tacagctaca
     4081 actcccggac cgaccgggtc acgccgatcc tcgagcacga ccccgcccgg ttctcaacgg
     4141 gtggctcgca attcctcacc acggatgagg agtcgtccgg tgtcatcgac gtgtcctcgc
     4201 tgctcggcga gggctcttac ctgctgacca cccaggtcca caaggcgagc gacgacgccg
     4261 agctcgtgga gcttggtcaa ctgcagctcg tccgggtcgc atggaaggac aacggcaagg
     4321 gctgagcgcc gatcgatgga cctgagtgcg tgacggagag cgtgacggag tgcgtgagag
     4381 ggggtgatca ccccctcaac cgcactccgt cacgcactct gcgtctcaga gcaggatggt
     4441 ggcgaaggtg ccgacccgtc ggaacccgac ggcctcgtag gtggcgatgg ccggggtgtt
     4501 gaagtcgttg acgtagaggg tcgcctcgga ggcgatgccg cgcagcacgg cagaggtcac
     4561 cgcggccatg agcgaggtcg ccaagccctg accgcgcaac gcaggcgcca gccacacgcc
     4621 ctggatctgc gcagcatcga cgcccaccga ccccacgtcg gccttgaaga gcacgcgccc
     4681 ctgatcgacc cacacgtagg tccgtcccga ccggatcagc ccggagacga cggcccgata
     4741 gttcgctgag gacccgacga acggggggta accgatctcc tcggtgaaca tcgcagcggc
     4801 cgccggaacg accaggtcga gctcgtccat ccgggccagc cgcacccggg gatcaatcgc
     4861 gaccccccag tcccccggat cgacgccggt cgacatgagc agttgttcgc gtctgatcgt
     4921 ccgagccggc ccccaggcgg gcccgagacg ctcccacaag ccggcgacct ggtcgaccgg
     4981 gccgaagatc gacgcgcagt gccgccgcca tcgacggacc ttggcggcga tcgcatcgag
     5041 gctcgggagg tcgcactcga cgggcaccag attggccgag gcccatgcga ccgagcgcag
     5101 gtctccgtcc tggccgggga ctccgagcag ggcacccggc atcgagtgca gggcaccctc
     5161 ttcgatccgg ccggcgacaa agcagttcgc ggccgggtcg cgcgcgcaca acgccaacgc
     5221 ctcgtcgcga tcggccggcg tcagtgcccg gacgggcgtg tgggtgcgca gcacggcata
     5281 cagagtgcca tgcctgcgcc cctaccgccc gattcgaggt actccggcgt cgccaggcaa
     5341 tgccgaaata cctcgaatcg gcgcccacgg ggcgaagact cgggaggagc cccaggccat
     5401 cgcgagcccg acaccggcca tgagccctgc accggcgagg aagcgaggcg ttgccacata
     5461 cagccccagc gggtcgttgt ccgcgctggc gaagcgaacc atcgccaccc cccagccaag
     5521 gactgcgaca accgcggcca cccgtgccca ggtcgggatg cgtcggtggg agagcaccgc
     5581 cccgcccacg agcgtcgacg gcaagaacac cgcgcctgca ccgagaaaga tgcccacccc
     5641 gacaagggca acgagg
//
LOCUS       DAOMZY010000243         5642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_13511_length_5642_cov_5.217648, whole genome shotgun sequence.
ACCESSION   DAOMZY010000243 DAOMZY010000000
VERSION     DAOMZY010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5642
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13511_length_5642_cov_5.217648"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="PLK64_12645"
     CDS             complement(<1..819)
                     /locus_tag="PLK64_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain-containing protein"
                     /protein_id="HPV80643.1"
                     /translation="MRPLWPAIWREPRPVGARPVGRADWLLVGGFEAAAVIEGLARPD
                     LAGQPYVTLLAMALMPALLWRRSHPLTTCLIGFGVAGLLSVIQLVAGTEDLGPTSMMV
                     VLILLYSLVRWGSGREILVGMPCIAAVVALGMYAAAATAPDLIGGSLLLLLIGSLAAV
                     FRARADLWQRQRREIRTQERLALARELHDTVAHHVSAIAVQAQAGGVIVRTQPAQAAQ
                     MLTAIEAEATRTLAEMRAMVRVLREDDWEDGREDGREGGRGDGRGDERAAYSPLP"
     gene            complement(816..1271)
                     /locus_tag="PLK64_12650"
     CDS             complement(816..1271)
                     /locus_tag="PLK64_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6326 family protein"
                     /protein_id="HPV80644.1"
                     /translation="MTTQTATAATTRTDPQVPLQLKLAAAWASFMFIYINVDLLGLYK
                     PGTVEGILKGKVFTFDITQTFMVGALAASAVPIMMIALSTALPARANRRTNLVAASLL
                     IPWMGFNLVGGTWPVYHGLGFALELVLLACIIRWAWTWHVTPAIPSLKR"
     gene            complement(1531..3909)
                     /locus_tag="PLK64_12655"
     CDS             complement(1531..3909)
                     /locus_tag="PLK64_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPV80645.1"
                     /translation="MRYPRPLSIVLAGALALGVAGVPMVTSAQAAQRTATAVRTVTDG
                     VTTFAVVDNPGGPTLSYIPGGGITLLTESTAAGTLTFKDFDADGQLDAFEDWRKPVNE
                     RAKALAAVLSIEQIAGLMLFSSHERNLAAGLTTAQRTYLSQDRLRNVLNASANDVTAN
                     VTWTNAAQAYAESLATAAEPYIPVNISSDPRSTAGSDTAYNAAGADISRWPSNIGLAA
                     TFSGDAMLEFAKITSQEYRAMGITTALGPQIDLATEPRWLRVDGTFGENVDLATSMAT
                     NYVEGSQNTYDANGTSVGWGADSITTMIKHWPGDGAGEGGREAHLNAGKYAVYPGNNF
                     AEHTEPFLAAKDSLAVMSSYSIAIAGDGSPLTGNRVGSAYDKVKLDLLRNADYDGVIC
                     TDWGVTTGYTDPNSRFGMAWGMEDATVEQRHYAALRAGVDMYGGNNRTAPVLAAYAMW
                     QADYAAGQNAISADERFRKSGERILRMIMAPGLFENPYLDLAASKAIVASPDKVAAGL
                     KTQADSIVLLKNDDKTIKASPAAKYKQQTVYIPSSIGYGFTNVFGATPDLTGPSLDAA
                     VAAQYFKKVLTDTPVLDANGKIVDYVMPDLRGVDVVLVGMRGPDNGDNFTGAGLTKDG
                     TFYPLSLQWAPYTADGPHVRKVSIAGDILADGSQQNRSYFGQTSQIGNEYDLVALNRT
                     LAAVEAIEKAQHKKIPVIVAVKAKTSFIPAEFESRVDALLVGYSVNGKVLIDAALGFA
                     EPHGRLPMTSPKDMDAVEAQLEDVGEDMTPYVDSLGNTYDFGFGLDWKGRIG"
     gene            4301..5383
                     /locus_tag="PLK64_12660"
     CDS             4301..5383
                     /locus_tag="PLK64_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HPV80646.1"
                     /translation="MAWSPEVPYNQLPPLPPEGIDLEPKPVLKMAIEARAALATLAQA
                     SRLLPNPGVLLNSLTLLEAQASSEIENIVTTSDALFKHAEIGGGDHATKEGLRYRSAL
                     FAGVEAMQHRPITATTAAQICSALHGREMAVRAVPGTRIANPATHEIIYAPPEGRDLI
                     LDKLAAWERFVHADDDLDPLVRMAVAHYQFEAIHPFHDGNGRTGRVLNILMLIEAGLL
                     DQPILYLSRSIITRKTDYYRLLRAVTSEQAWTDWVLYMLDVVRESAESTTRKIAAIRT
                     SQHDIAERARDATPGGRDSRFLDVLFEQPYCRISTVVDRCDVSRQTASTWLHALVGAG
                     LLTDLKIGRDVVFLNRDLLQVLTRPE"
     gene            complement(5436..>5642)
                     /locus_tag="PLK64_12665"
     CDS             complement(5436..>5642)
                     /locus_tag="PLK64_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80647.1"
                     /translation="QTGGGGENVIKHRHPMILSEPITSGPGLAFGLPLSTPSAVGLDF
                     RIPSANLSASSTCSQDVLGIVAIS"
BASE COUNT          952 a         1881 c         1872 g          937 t
ORIGIN      
        1 cggcagcggg gagtatgccg cccgctcgtc cccgcggcca tcccctcgcc cgccctcgcg
       61 cccgtcctcg cgcccgtcct cccagtcgtc ttcccgcagc acgcggacca tggcccgcat
      121 ctccgcaagg gtgcgcgtcg cctcggcctc gatcgcagtc agcatctgtg ccgcctgcgc
      181 gggctgggtc cggacgatca caccgccggc ctgagcctgc accgcgatgg ccgagacgtg
      241 gtgggcaacc gtgtcgtgca gttcgcgggc cagcgccagt cgctcctgag tgcggatttc
      301 gcggcgttgg cgctgccaga ggtcagcgcg tgcgcggaag accgctgcga gagagccgat
      361 gagcagaagg aggaggctgc caccgatgag gtccggcgca gtggccgccg cggcatacat
      421 gccgagagcc acgaccgcag cgatgcacgg catacccacg aggatctctc gccccgagcc
      481 ccagcggacc aaggagtaga gcaggatcag aacgaccatc atcgacgtgg ggccgaggtc
      541 ttcagtgccg gcgaccagct ggatgacgga gagcaacccc gcgacaccga agccgatgag
      601 gcaggtggtg agcgggtggc tgcggcgcca gaggagggct ggcatcaggg ccatggcgag
      661 cagggtgacg tagggctgcc cggcgagatc tggccgagcg aggccctcga tcactgcggc
      721 cgcctcgaac ccgccgacga gaagccagtc ggcacggccc actggccggg cgccgacggg
      781 ccgaggctcg cgccagatag cgggccagag gggtctcacc gcttcaggct agggatcgcc
      841 ggcgtgacgt gccaggtcca ggcccagcgg atgatgcacg cgaggaggac cagctcgagg
      901 gcgaacccga ggccgtggta gaccggccac gtcccgccga ccaggttgaa ccccatccag
      961 gggatgagca gggaggcggc gacgaggttc gtccgacgat tggcgcgcgc gggcagggcc
     1021 gtcgagagcg cgatcatcat gatcgggacc gctgaggctg ccagcgcgcc gaccatgaag
     1081 gtctgcgtga tgtcgaaggt gaagaccttg cccttgagga tgccctcgac ggtgcccggc
     1141 ttgtagaggc cgaggagatc gacattgatg tagatgaaca tgaagctggc ccaggcagca
     1201 gcgagtttca gctggagggg gacctgcggg tccgtgcggg tcgtcgcggc agttgctgtc
     1261 tgcgtggtca tcggggtgag cccttcgtgg tgcgtggagt gggtgactcc accacgatgg
     1321 ctgacctggc ctgcggactc atcagcccgc gggcccgtcc tggaccggcc gaaagaacgt
     1381 ccggcgaacc gtcctttcgg cgggagttca ggtcgcgcgg gagtccacgt cacggcgggc
     1441 ccggcgcaca cggcataccc atgctccgac gacgtagcgc caccgacccg ggggaacggg
     1501 gccggtggcg ctacgaacga cctgtcggcg tcagccgatc cgacccttcc agtccagacc
     1561 gaagccgaag tcgtaggtgt tgcccaggga gtcgacgtac ggcgtcatgt cttcgccgac
     1621 atcttcgagc tgagcctcga ccgcgtccat gtccttcggc gaggtcatcg gcaggcgacc
     1681 gtgcggttcg gcgaagccga gggccgcgtc gatgagcacc ttgccgttga ccgagtagcc
     1741 gaccaagagc gcgtcgacac gtgactcgaa ctcggccggg atgaaggagg tcttggcctt
     1801 cacggccacg atcaccggga tcttcttgtg ctgcgccttc tcgatcgcct cgacggcggc
     1861 gagggtgcgg ttcagcgcca cgaggtcgta ctcgttgccg atctgtgagg tctgaccgaa
     1921 gtaggaccgg ttctgctgcg acccatccgc gaggatgtca ccggcgatcg acaccttgcg
     1981 cacgtgcgga ccatcggccg tgtacggcgc ccactgcaac gacaacgggt agaaggtgcc
     2041 gtccttcgtc agccccgcgc ccgtgaagtt gtcgccattg tcgggcccgc gcatgccgac
     2101 cagcaccacg tcaacgcccc tgaggtcagg catcacatag tcaacgatct tgccgttcgc
     2161 gtcgaggacc ggcgtgtcgg tcagcacctt cttgaagtat tgagcggcca cggcagcgtc
     2221 caggctcggt ccggtcagat cgggcgtggc gccgaagacg ttggtgaagc cgtacccgat
     2281 cgagctgggg atgtagaccg tctgctgctt gtacttcgcc gccggcgacg ccttgatcgt
     2341 cttgtcgtcg ttcttcagca ggacgatcga gtcggcctgg gtcttcaggc cggccgccac
     2401 cttgtcgggg ctggccacga tcgccttgga ggccgccagg tcaaggtagg ggttctcgaa
     2461 caagcccggc gccatgatca tgcgcaggat ccgctcaccc gacttgcgga accgctcgtc
     2521 ggccgagatc gcgttctggc cggctgcgta gtcggcctgc cacatggcgt acgccgccag
     2581 caccggcgcc gtccggttgt tgccgccgta catgtccacg cccgcacgca acgccgcgta
     2641 gtggcgctgc tccacggtgg cgtcctccat gccccacgcc atgccgaacc gcgagttggg
     2701 gtcggtgtag ccggtggtca caccccagtc ggtgcagatc acgccgtcgt agtcggcgtt
     2761 ccgcagcagg tcgagcttca ccttgtcgta ggccgacccg acccggtttc cggtcaacgg
     2821 cgaaccatcg cccgcgatcg cgatcgagta cgagctcatc acggcgagcg agtctttcgc
     2881 cgcgaggaac ggctcggtgt gctcggcgaa gttgttgccg gggtagacgg catacttgcc
     2941 cgcgttgagg tgcgcctcgc ggccgccctc accggcgccg tccccgggcc agtgcttgat
     3001 catcgtcgtg atcgagtcgg caccccaccc gacactcgtc ccgttggcgt cgtaggtgtt
     3061 ctgcgatccc tcgacgtagt tggtggccat cgaggtggcc agatcgacgt tctcaccgaa
     3121 cgtgccatcg actcgcaacc agcgtggctc ggtggccagg tcgatctgcg gaccgagcgc
     3181 cgtggtgatg cccatggcgc ggtactcctg cgaggtgatc ttcgcgaact cgagcatggc
     3241 gtctcctgag aaggtggccg ccagaccgat gttcgagggc caccgcgaga tgtccgcccc
     3301 tgccgcgttg tatgccgtgt cgctcccggc ggtggaccgc gggtcggaag agatgttgac
     3361 cgggatatag ggctcggccg cggtggccaa agactcggca tacgcctgcg ccgcattcgt
     3421 ccaggtcaca tttgccgtga catcgttggc cgaggcgttc aagacattgc gcagacggtc
     3481 ctgcgaaaga taagtccttt gggctgtggt cagtccggcc gcaagatttc gctcatgaga
     3541 gctgaacaac atcagtccgg cgatctgctc gatcgacagc acggcggcca gggccttggc
     3601 ccgctcattg accggcttgc gccagtcctc gaaggcatcg agttggccgt cagcgtcgaa
     3661 gtccttgaac gtcagggttc ccgcggcggt ggactcggtc agcagcgtga tgccgcctcc
     3721 ggggatgtag ctcagcgtgg gcccgcccgg attgtccacc acggcgaacg tggtcactcc
     3781 gtcagtcacc gtgcgcacgg cagtcgctgt gcgctgcgcc gcctgggcgg aggtgaccat
     3841 gggcaccccc gcaactccga gggccaaagc tcccgcaaga actattgaca aaggacgtgg
     3901 atatcgcatc gcgactccat ctgtttgggg ccgaacttcg atgctcggcc gggagagtgt
     3961 cgcgcacgtt accggagggg cgcacttctc gcgcggcgag aatccgggac gtgcaaggcg
     4021 ccaattcttg ggacccaccg gggcccgccc cggggcccga tgccgcctgc gacggcggct
     4081 ccggcgcaca cggcatacca atgttccgcg cggtgagcat gcggtgagca cgggagggtg
     4141 ggagaccacc caccattggt atgccgtgtg ctcaccgacg cccagtgggg acgagacgtt
     4201 ctgaacggca gcgcgggctg accgtcaggc acgcggcata cgtgttaagt ttctgccaga
     4261 ttattgacgt gtttgcccaa cgtgtcgaaa gatgtgaccg atggcctgga gccccgaggt
     4321 gccctacaac cagctgcccc cgctgccgcc cgaggggatc gacctcgagc ccaagccggt
     4381 cctcaagatg gcgatcgaag cacgggccgc actggccacc cttgcgcagg cgagccggct
     4441 cctcccaaac ccaggcgtcc tgctcaacag cctcaccttg ctggaggccc aggcgagctc
     4501 ggagatcgag aacatcgtca cgacgagtga tgccctgttc aaacacgctg agatcggggg
     4561 cggcgaccac gccacgaagg aggggctgcg ttatcgaagc gccctcttcg ccggcgtcga
     4621 ggcgatgcag caccgaccca tcaccgccac gactgccgcc cagatctgct ccgccctgca
     4681 tggccgggag atggctgttc gagccgtccc cggcacccgg atcgccaatc cggcgacgca
     4741 cgagatcatc tacgcgccgc ctgagggccg agatctcatc ctcgacaaac tggccgcgtg
     4801 ggaacggttc gtccacgcgg atgacgacct tgaccctctg gtcaggatgg ccgtggcgca
     4861 ctatcagttt gaggcgatcc atcccttcca cgacggcaac ggacgaaccg ggcgagtcct
     4921 caacatcctc atgctcatcg aggcgggcct gctcgaccag cccatcctct acttgtcgcg
     4981 gtcaatcatc actcgcaaga ccgactacta ccggctccta cgtgccgtga cctccgagca
     5041 ggcgtggacc gactgggttc tctacatgct tgacgtcgtc cgtgagtcgg ccgagtccac
     5101 gacccgcaag atcgcggcga tccgcaccag ccaacacgac atcgccgaac gcgcccgcga
     5161 cgcgaccccc ggcggtcgcg actcgcgttt cctggatgtc ctcttcgagc agccctactg
     5221 tcgcatcagc accgtcgtcg atcgctgcga tgtgtcgcgg cagaccgcct ccacctggct
     5281 gcacgcgctt gtgggggcgg gcctcctcac cgacctcaag atcggccgcg acgtcgtctt
     5341 cctcaaccgt gatctgctgc aggtcctcac ccgcccggaa tagctccacg agcccggcca
     5401 gcgggtgaca ctccaaccaa cggccgctca acgtgttaag aaatcgcgac gatacctaac
     5461 acgtcttggg aacatgtcga ggaagccgac aggttcgccg acggtatccg gaagtccaag
     5521 ccgacggccg aaggcgtcga gagcggcagt ccgaacgcca gacctggacc ggatgttatc
     5581 ggttctgata agatcatcgg gtggcgatgc ttgatgacgt tctcgcctcc gccgcccgtt
     5641 tg
//
LOCUS       DAOMZY010000244         5608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_13610_length_5608_cov_6.882226, whole genome shotgun sequence.
ACCESSION   DAOMZY010000244 DAOMZY010000000
VERSION     DAOMZY010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13610_length_5608_cov_6.882226"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            575..2506
                     /locus_tag="PLK64_12670"
     CDS             575..2506
                     /locus_tag="PLK64_12670"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPV80648.1"
                     /translation="MLLKDVRIQDYRSVEDSGTVPIEIDVTCLVGKNESGKTAFLQAL
                     HLLNPLNPIQNKTQYDEVMDYPSRKSSAYKKTREANPATVVTADFTLEEHELDAVRAE
                     FGPDCLTGTTVTVKAGYYGNKTFSVGIDTGAALKHLTAAIEVPSAELKPIKAATTVAA
                     LKAALEAVAEPSAAVTALLARIAGWRKESLSHHLIDTYWSEWLPVFFYFDDYSTMRGR
                     VSLPHIKAREEARTLEESEKTFLSLLATVDADLADFETANFERLTRELEGAANGITDK
                     VFEYWTQNKELRLEIRVSSADPDDEPPLNQGPIVNVRVYNPRHRVSVPFDERSRGFVW
                     FFSFFAYFSDVEQQDGRRTVLLLDEPGLALHATAQSDFLRFIDGELAQTHQVLYSTHS
                     PFLVKPDRFDRVRTVQDVDGRGTQVSSDIFRTDSETVFPLQTALGYELAQTLFVGPDC
                     LLVEGPSDLLYLQILTQACEVAGLTGLDPRWVVTPVGGADKLGTFVSLLGANQLNVAA
                     LIDSNPKDKQRIAALQDNGHLKGRALIELSEITGTDEADIEDLFNPAFYVALVNASYQ
                     GELSKPLKASDLKSKAPRITARIEQHLRENGIANGRLNHYRPAAHLLREQTDLIPKID
                     KDTLQRADALFKRLNSLLD"
     gene            complement(2611..3369)
                     /locus_tag="PLK64_12675"
     CDS             complement(2611..3369)
                     /locus_tag="PLK64_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HPV80649.1"
                     /translation="MVELATTMFARETGFSGPDLHRLFAEYTEGLGEYTGWGGGSESR
                     WQIFQRGLKLLSFGQQVQFLLGLCEYDGNIKHGRPSDEDIAKLRALLLGQSAPGSDAA
                     AERLAALTDWKAVQDSWRQALDKIHSDPEGAITATRTLLESTCKHICDERGVSYENNW
                     DLSRLYKATAGAMEIAPDQHSEQVIKQILSGVSTVVTGLGSLRNSLSDAHGRGKRAVR
                     PAPRHAKLAVNAGFAVAGFLIDTHIERPSPTVRS"
     gene            complement(3389..>5608)
                     /locus_tag="PLK64_12680"
     CDS             complement(3389..>5608)
                     /locus_tag="PLK64_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HPV80650.1"
                     /translation="VTLARILFLMFGDDTEMWHTDAFRNVIQHETLPDGSNLAQVLND
                     LFTFLDTANPSSVPNGFEGFKYVNGGIFEERIELPPLGKDFRAAVLDACAVDWSSISP
                     AIFGSMFQSVRDAATRRALGEHYTSEENILKTLNPLFLDELRAELAAALARDTTQKKV
                     NALNKLWDRLGDIRFMDPACGCGNFIIVAYRELRAIELQVMEALYDLRDKHQLSLDAK
                     SDLKVTLDHFYGIEIDEWPARIAETAMFMIDRQCDLKLKERFGQAPERLPIQREAKIV
                     VGNALRIDWEQVLPAGNDVVVAGNPPFSGRHTRSKEQATDLANAWQVKNAGHLDYVTG
                     WHAIASRYLSGTQGVFAFVSTNSVTQGEPVALMFPKILEAGWRIKFAHRTFAWTSEAT
                     GKATVHCVILGFTQQTTDKPRLFAYDTPTSDPVEMPVVDSINPYLVDGPSIFVRKRTG
                     RPLAPHLPVMEYGSMPNDGGYLIFDGAGAAEALQDPIVAKYLRRYVGASELIHGVERW
                     CLWLVDMDPADVKRSSLLRSRIEAVRATRLASRNPDTVQRAATPHLFWFINQPTTAYL
                     CVPQVFSENRQWVTAARLNSDVIANNRVYTCPDPDGFGFAVISSAMFLTWQKTVGGRL
                     ESRYNFSGTIVWNNLPLPPVDVDVRAAMIEAGDAVIQSRSLYPDRSLADLYEPRRLPG
                     EVAAAHETLDRVVDELFGLRVDERSEAGRQHALFEQFAKMQALASPGATIRRARGRQ"
BASE COUNT         1021 a         1845 c         1729 g         1013 t
ORIGIN      
        1 aaactgtaag aagtgccgtt gattccatga attagccgac gttatgcact tcgcgcgtgt
       61 cgcatcacgg tgtttgaccc tcccccggtg cccgaccagc gggtgacccg agcaccactt
      121 ggatcccgtg accgctcgcc aggccagctg acggggcggc cattggaaac tcgacccgtc
      181 ggccaggtga ccgagcagct cgagtgggac atcgcctctg ctgacccttg cccgagcgtc
      241 acgggcagga agtgcttcgc gtggggcacc cggctgggcg ggcgctggac gactcgccgg
      301 acatgcttga tgagcgggtg ttgtcccggc cgcccgcggg cctagttcgc gttccgagag
      361 gtccgagaga gctgccccag gagtgccgcg ctggcacggt gtggacctgt gtctacccga
      421 acatttgccc ggacaagacc cgcgggtcgg cgactctcgg gctgctgact actgaacagc
      481 gcagatgtct tgtcggcccc atgtgttagc catagggtca agcaggcgcg tgccggacga
      541 gccgggacgc ccgcgccgac gaggggatag agcagtgctg ctcaaggacg ttcgtattca
      601 ggactaccgc agcgtcgagg actccggaac cgtcccgatc gagatcgacg tcacctgcct
      661 cgtcggcaag aacgagagcg gcaagaccgc gtttctccag gcgctgcacc tgctcaaccc
      721 actgaacccg attcagaaca agacgcagta cgacgaggtc atggattacc cgagcaggaa
      781 gtcctccgcc tacaagaaga ctcgcgaggc gaacccggcg accgtcgtga ccgcggactt
      841 cactctggag gagcacgagc tcgacgccgt ccgcgcggag ttcggccccg actgcctcac
      901 cggcacgacc gttacggtca aggcgggcta ctacggcaac aagacgttct cagtcggcat
      961 cgacaccggc gccgccctca agcacctcac cgctgccatc gaggttccga gcgcagaact
     1021 caagccgatc aaggccgcga cgactgtcgc cgcgctcaag gcggcgctgg aagccgtcgc
     1081 agaacccagc gccgccgtca ccgcactgct cgcgcgcatc gccggctggc gcaaagaatc
     1141 gctcagccac cacctcatcg acacctactg gagcgagtgg ctcccggtgt tcttctactt
     1201 cgacgactac agcaccatgc gcggacgcgt ctcccttccc cacatcaagg ccagggagga
     1261 ggcgaggacg ctggaggagt ccgagaagac gtttctctcc ctcctcgcca cggtggacgc
     1321 cgatcttgcc gactttgaga cggccaactt cgagcgactc acccgcgagc tcgaaggggc
     1381 cgcgaacggc atcaccgaca aggtctttga gtactggacc cagaacaagg aactccgcct
     1441 ggagatccgg gtctcctccg ccgaccccga cgacgagcct ccgcttaacc aagggcccat
     1501 cgtcaacgtc cgcgtctaca acccccgcca ccgagtcagc gtccccttcg acgagcggtc
     1561 acgcggcttc gtgtggttct tctccttctt cgcgtacttt tccgatgtcg agcagcaaga
     1621 cggacgccgg accgtgctcc tgctcgacga gcccggcctc gccctgcacg cgaccgccca
     1681 gagcgacttc ctgcgcttca tcgacggcga gctcgcccag acgcaccagg tgctctacag
     1741 cacccactcg ccgttccttg tcaagcccga ccgcttcgac cgcgtccgca ccgtccagga
     1801 cgtcgacggc aggggcaccc aggtcagctc ggacattttc cgcaccgaca gcgagacggt
     1861 cttcccgctc cagaccgcac tcggctacga actcgcccaa actcttttcg tcgggccgga
     1921 ctgccttctc gtcgaaggtc cgtctgacct gctctacctc cagatcctga cccaagcgtg
     1981 cgaggtcgcc ggcctcacag gcttggaccc ccgctgggtc gtcacccctg tcggtggcgc
     2041 cgacaagctc gggacgttcg tcagcctcct cggcgccaac cagctcaacg tcgccgcgct
     2101 catcgactcc aaccccaagg acaagcagcg catcgccgcg ctccaggaca acggccacct
     2161 caagggccgg gcactgatcg agctcagcga aatcacgggc accgacgagg ccgacatcga
     2221 agacctcttc aaccccgcct tctacgtcgc actcgtcaac gcctcatacc agggcgagct
     2281 ctccaagccc ctcaaggcgt ccgacctcaa gtccaaggca ccccgcatca ccgcccgcat
     2341 tgagcagcac ctgcgcgaaa acggcatcgc caacggccgg ctcaaccact accgccccgc
     2401 cgcacatctg ctccgtgagc agaccgacct cattcccaag atcgacaagg acacgcttca
     2461 gcgcgccgat gccctattca agcggctcaa cagcctcctc gactgagctc ggccatcatt
     2521 gggttttgca ccggggccag ttgatcagat ttcggtgcct accttcgtac agtcttcgcg
     2581 ccgcctcgac agcgcgccgt gcgaggatcg ctaacttcgc acggtcgggc tgggccgctc
     2641 tatgtgcgtg tcgatgagga atccggccac ggcgaagccc gcgttcacgg cgagtttggc
     2701 gtggcgaggg gcaggcctga cagcgcgctt cccgcggcca tgcgcgtcgc tgaggctgtt
     2761 ccgcagggac cccaggcccg tgacgacggt cgagacgcca gacaaaatct gcttgatcac
     2821 ctgttcactg tgctggtccg gggcgatctc catggctccg gcggtggcct tgtagagccg
     2881 gctcaggtcc cagttgttct cgtacgagac gccccgttca tcgcaaatgt gcttgcaggt
     2941 gctctcgagc agggtccgcg tagccgtgat cgctccctcc ggatcagagt ggatcttgtc
     3001 gagtgcctga cgccaagagt cttggaccgc cttccagtcc gtcagggcgg ccagtcgctc
     3061 ggcggcagca tccgatccgg gtgcgctctg gccgagcagc agggcacgca actttgcgat
     3121 gtcctcatcg gatgggcggc cgtgcttgat gttcccgtcg tactcgcaca ggccaagcag
     3181 gaactggact tgctgcccaa acgacaggag cttcaaaccc cgttggaaga tctgccaccg
     3241 cgactccgaa ccgccacccc agccggtgta ctcgccaagg ccctccgtgt actcggcgaa
     3301 gagtcggtga aggtcagggc cactgaagcc cgtctcgcgc gcgaacatcg ttgtcgccag
     3361 ctcgaccact cgcacgggca agtggccact actgtcgacc acgtgcgcgc ctaatcgtcg
     3421 cgccaggcga ggcaagggcc tgcatcttgg cgaactgctc gaacagggca tgttgtcgtc
     3481 cggcttcact ccgctcatcg acgcggagcc caaacagctc gtcaacgact cgatcgagcg
     3541 tctcatgcgc ggccgcgacc tcccccggaa gccgacgagg ctcgtacaag tcggctagtg
     3601 accggtcggg gtagagagat cgtgactgaa tgacggcgtc gccagcttcg atcatcgcgg
     3661 cgcggacgtc gacgtcgacc ggcggcagcg gcaggttgtt ccaaacgatg gtcccagaga
     3721 agttgtaccg agactccaat cttccgccga cggtcttctg ccaagtcagg aacatagcgc
     3781 tgcttatgac cgcaaagccg aagccatcag ggtcggggca ggtgtacacg cggttgtttg
     3841 cgatgacatc gctgttgagc cgtgcggcgg tgacccactg ccggttctcg gagaaaacct
     3901 gcgggacgca gaggtaggcc gtcgtgggct gattgatgaa ccagaacagg tgcggggtgg
     3961 ctgcgcgctg aactgtgtcg gggttcctgc tcgcaagtcg cgtcgcccgc accgcctcga
     4021 tgcgagatcg cagaagtgac gatcgtttca cgtcggctgg gtccatatcc acgagccata
     4081 ggcaccacct ttcgacgccg tggatcaact ccgaagcgcc gacatatcgc cggaggtact
     4141 tggcaacgat tggatcctgt aaggcttcag cggctcccgc accatcgaag atgaggtagc
     4201 caccatcatt cggcatcgat ccgtactcca tgacgggcag gtggggtgcg agtggcctgc
     4261 ccgtgcgctt ccgaacaaag atcgacggac cgtcgaccag gtatggattg atggagtcca
     4321 caacaggcat ctcgacggga tctgatgtcg gcgtgtcgta ggcgaacagg cgcggcttgt
     4381 cggtcgtctg ttgggtgaac ccgaggatga cgcagtggac cgttgctttc cccgttgcct
     4441 ccgacgtcca agcgaaggtg cggtgcgcga acttgattcg ccaccctgcc tccaggattt
     4501 tcgggaacat gagtgcgact ggctctccct gtgtgaccga gttggtcgac acgaacgcga
     4561 agacaccctg agtgccgctg aggtaacggc tcgcgatcgc atgccacccg gttacgtagt
     4621 ccaggtgtcc agcgttcttc acctgccaag cgttcgctag atcggttgcc tgctccttgc
     4681 tcctcgtgtg tcgcccggaa aagggtgggt ttccagcaac gacgacgtca ttgccggccg
     4741 ggaggacttg ttcccagtcg atgcgtagcg cgttgccgac gacgatcttg gcttcacgct
     4801 ggatggggag gcgttcgggt gcctggccga atcgttcctt caacttcaga tcgcactgtc
     4861 gatcgatcat gaacatcgcc gtctcggcga ttcgggctgg ccactcgtcg atctcgatgc
     4921 cgtagaagtg gtcgagggtg accttcaggt cggacttcgc gtcgagggag agctggtgct
     4981 tgtcccgtag gtcgtagagc gcctccatga cctggagctc aatggctcgg agctcacggt
     5041 aggcgacgat gatgaagttg ccgcagccac aggctgggtc catgaagcgg atgtcgccga
     5101 gtcggtccca gagtttgttg agggcgttga ccttcttctg ggtggtgtcg cgggcaaggg
     5161 cggccgcgag ctctgcacgc agttcgtcga ggaacagcgg gttcagtgtc ttgaggatgt
     5221 tctcctcgga cgtgtagtgc tcgcccagcg cgcgtcgggt tgcggcgtct cgcactgact
     5281 gaaacatgga gccgaagatg gccgggctga tgctggacca gtcaacggcg caggcgtcca
     5341 agacggccgc gcggaagtct ttgcccagtg gcggtagctc gatcctctcc tcgaagatcc
     5401 cgccgttgac gtacttgaag ccttcgaagc cgttcggcac gctgctgggg ttggccgtgt
     5461 cgaggaacgt gaagaggtcg ttcaacacct gggcaaggtt cgagccgtcg ggcaaggtct
     5521 cgtgctggat gacgtttcgg aacgcgtcgg tgtgccacat ctcggtgtcg tcgccgaaca
     5581 tgaggaagag gatccgggca agggtcac
//
LOCUS       DAOMZY010000245         5606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_13615_length_5606_cov_6.697712, whole genome shotgun sequence.
ACCESSION   DAOMZY010000245 DAOMZY010000000
VERSION     DAOMZY010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13615_length_5606_cov_6.697712"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            933..1466
                     /locus_tag="PLK64_12685"
     CDS             933..1466
                     /locus_tag="PLK64_12685"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV80651.1"
                     /translation="MIALEVPIVAFGKSFDRSRFTSGNADLDDWLRTKAGQGERSDNT
                     RTFLALDPGGERVIGYYATTTYRLELDEAARALGAGKRRYPVPAVLLARLAVDGEWAG
                     RGVGRTLLVHALRQIAEASRSVGFEVVVVHAADESAVTFYARYGFTAFVSHPRHLLMP
                     TKQLRATFEAERLGHPR"
     gene            1537..1815
                     /locus_tag="PLK64_12690"
     CDS             1537..1815
                     /locus_tag="PLK64_12690"
                     /inference="COORDINATES: protein motif:HMM:NF020265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HPV80652.1"
                     /translation="MSTKTDRLNLRCSSATLATLREAAEVQDQDLTSFILGAALDRAR
                     TVLAEDRVLRLDPHEVLQLERALDAEPAAIPQLAALLRSVRASASARV"
     gene            complement(1882..2508)
                     /locus_tag="PLK64_12695"
     CDS             complement(1882..2508)
                     /locus_tag="PLK64_12695"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HPV80653.1"
                     /translation="MPKLVDHDERRHEIARALWSVVARGGFEAASARTVAAEGNWSLG
                     ALRHYFSSQDELVEFAARELIDGAAARIATVLETMPAGLPRCRMALEQLLPLDDVRRG
                     EVRAWLAILVKAEVSPSLAELREVALDGERQLCRLVVAELSGHPYPTSPSDWPAALEY
                     AAVDLHAWIDGHCLQAASSRQLLPADAARAALDRYLRQLSVTVAPGTA"
     gene            2566..3291
                     /locus_tag="PLK64_12700"
     CDS             2566..3291
                     /locus_tag="PLK64_12700"
                     /inference="COORDINATES: protein motif:HMM:NF022589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HPV80654.1"
                     /translation="MTPELAAALVGLALVDATSFGTLVLPTLMLVQPRVRASRVVLYL
                     AAISAFYFVLGVALLFAAERVLALVAATERSRVVDMVQLVVGLALLAGSFWPDLPWVK
                     QRRQERDAAGGGRRPGWRDRVLGPEARSRTVIAVAVGAGLVEAASMLPYLGAVGMLTS
                     SVASTPERLAALASYVVVMCLPALILLAARLLLGARVDGALGRLARWLDRQSRNAIWW
                     VVGVVGFLIARDAASRIWPGSMG"
     gene            3495..4700
                     /locus_tag="PLK64_12705"
     CDS             3495..4700
                     /locus_tag="PLK64_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV80655.1"
                     /translation="MLSAYVPLFRVPGALRFIAGTALSRVGGSMFGVAIIVMLSERRG
                     SFGLAGAISAVGVGTLAVAGPFIGRLIDKHGQRRIALPFIVSSAIFGTSAAVLSIVNA
                     PVWTIFVAYALSAVLPEPGPMSRARWAHIYRDDADQLHTAMSFEQVADEGSFVIGPVV
                     AVLVSTLWFPEAGLLIAELLFTVGMVAFLTATATEPPVVAHENRPAGLAIRWPGLMLV
                     ATALTMTGVIFGSNEVIAVAFADARGNTGFSSVVLGGFALGSTLAGIVFGSRKFNSTL
                     SRRLLLAAAGMFVLEAPAFFMSDLWPLAVVMFIAGSATAPMLITSLSLAQVLVPKAMV
                     TEGMAVAITGILIGISGGAAVGGWAIEAWGAQRAYAVPILAGAAALAIIVARFRHVQR
                     AELTASAEA"
     gene            complement(4720..5082)
                     /locus_tag="PLK64_12710"
     CDS             complement(4720..5082)
                     /locus_tag="PLK64_12710"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HPV80656.1"
                     /translation="MNAVIVVSTAVIVALTAAALLQFALTKSGSIDRNAMIGIKTSAT
                     LASDSAWKAGHVAAQPALMTAAVTGAAALVTVLVAWLLAPQAELALAVVSATGLVVML
                     GLFVRVAFSANHAARNAV"
     gene            complement(5309..>5606)
                     /locus_tag="PLK64_12715"
     CDS             complement(5309..>5606)
                     /locus_tag="PLK64_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80657.1"
                     /translation="PAALRSLRAALRPGGYLCIADLDADGGAYHAGHSHFHGHHGFDR
                     EALAAQLIEGGFVDPHLRTVYHLRKPVDGVEQDFPLFLAVAQVGQVGQAGPDRA"
BASE COUNT          845 a         1805 c         1948 g         1008 t
ORIGIN      
        1 cgtttgggct gatccacggc ttcgggtttg ccagcgtgct gcgcgacttg ggcggtgatc
       61 ggttcggcgc gctgcccatc aaccgttact tggaatgtaa cccgagagtc tggctcaagt
      121 gagtcccggg ttacgccgac ctccgcgact agttgagtcg ctcctccggg gacaaggtag
      181 tcgacgggtc ctgtttggga gcatcccgac atgaggttga taagggagtt tgggtaggtg
      241 acgtcggaaa agtccacgac ccgttgttcg agagatgagt cgacgggctg aagatcaacg
      301 aggtacgttc ggccagaatt gccggtggct gcaacgccag tcccggtgtt cgagccgatg
      361 gtaggcgtca ccgtgcgggt gacggtctcc gtcgtcgtgg atagtgccgt ttgcgtcacc
      421 gtgatggtgg tggtgggaag agccactacg gaaggcagat tgcccgagcg cgccgccagc
      481 agtgttgcga cagctccgat cactccggca acaagcgttg cgcccgcgtt gagccacccc
      541 gtccgcgcgg gatgactacc gtgcccgcct ggatgtgcca ccgtcgcctc caaagaattc
      601 aactgagaat tatcgattgg atgctacgac atcgggtccc gcgcgggggt gggtgaccta
      661 actcatggcg agagcgtgcg gccgaagcga tctcggatgg gtgctgccga gcgaggcgtg
      721 ttgaggttgc acctgacgcg gggatgaaca agggtgtgtg gataggggtg accactccgg
      781 ccacggcctg cccaagattg gcgaggaggg ctttggtgtc gaccgactcg ctgggcgagg
      841 acctcagggt gggggatccg ggcagccatt ccgccgtgtt gagtgagcct gttggcgaac
      901 aatcgatgaa agtcttcgat gtggagtgac tcgtgatcgc ccttgaagtc cccatcgttg
      961 ccttcggcaa gagctttgat cgctcgcggt tcacaagtgg caatgctgac ctcgacgact
     1021 ggctcaggac caaggccggc cagggggaac gctctgacaa cacccggacg ttccttgcgc
     1081 tcgacccggg tggcgaacgg gtgatcggct actacgcgac gacgacgtac cgcctggaac
     1141 tcgacgaggc ggctcgggct ctgggtgcgg gcaagcgtcg atacccggtg ccggccgtcc
     1201 tcctggctcg gctggcggtt gacggcgagt gggcgggcag aggagttggg cgaacgctct
     1261 tggtgcatgc gctgcgccag atcgctgagg ccagccgctc ggtcgggttc gaagtcgtcg
     1321 tcgtccacgc cgcggacgag agtgccgtga ccttctacgc gcgctatggc ttcacggcct
     1381 tcgtctctca tccccggcat ctgctcatgc ccacgaagca gttgcgggcc acgtttgagg
     1441 cggaacgctt agggcatccg cgctagacgt gttaccgtag tgagtacggt atacgtacaa
     1501 ggggcgcacg aaacccctcc tgatccagga gcgagcatgt ccaccaagac cgaccgactc
     1561 aacctgcgtt gctcatcggc gacgctcgcc actctgcgtg aggcggccga ggttcaggac
     1621 caagatttga cttctttcat tctcggcgcg gcgttggacc gggctcggac cgtgttggcc
     1681 gaggaccgcg tgttgcgcct tgacccgcat gaagtgctgc agttggagcg ggcgctggac
     1741 gccgagcccg cggcgatccc tcaactcgct gccctgctgc gcagtgttcg cgcctccgca
     1801 tcggcgcggg tttgacaggc gcccgactcg actcatcacg catcggcggg ctttactgca
     1861 tgaagtgctt cacttcggaa gtcaggcggt gcctggggcg acggtgacgg agagttggcg
     1921 gaggtaacga tcaagagccg cacgggcagc gtcggcgggc aacaactggc gtgacgacgc
     1981 tgcctgtagg cagtgcccgt cgatccaggc gtgaaggtcg acggcggcat actcgagggc
     2041 ggccggccag tcgctcggtg aggtggggta ggggtggccg ctgagttcgg cgacgactaa
     2101 ccgacacagt tggcgctcgc cgtccagcgc gacctcgcgc agttccgcca ggctagggct
     2161 cacctcggcc ttgaccagga tcgccagcca ggcgcgaact tcgccgcggc gaacgtcgtc
     2221 gagagggagg agttgttcga gggccatccg gcatctcggc aaccccgctg gcatcgtttc
     2281 gaggacggtc gcgatgcgcg cggcggcgcc gtcgatgagc tctcgcgcag caaactcgac
     2341 gagctcgtcc tgcgacgaga agtagtggcg cagcgcgccc aaagaccaat tgccttcagc
     2401 ggcaacggtt cgcgcgctcg ccgcctcgaa tcctcctcgg gcgacgaccg accacaacgc
     2461 gcgtgcgatc tcgtggcgcc gctcgtcatg gtccacgagc ttgggcacgg ctcaccctcg
     2521 cacgatctct cgccgttggc aatacgaatg tgttacaaaa ggggcatgac gcccgaactt
     2581 gcggccgccc tcgtcggtct cgcgctggtc gatgcgacca gtttcgggac gctggtcctg
     2641 cccacgctca tgctggtgca gccgcgggtt cgcgccagcc gtgtggtgct ctacctcgcc
     2701 gccatctcgg ccttctactt cgtcctcggc gtcgccctcc tgttcgcggc cgaacgcgtc
     2761 ctggctctgg tcgcagccac cgagcgaagc cgggtcgtcg acatggtgca actcgtggtg
     2821 ggtctggccc tcttggcggg aagcttctgg cccgacctgc catgggtcaa gcagcgccgc
     2881 caagagcgcg acgctgcggg cggcggccgg cggcccggct ggagggatcg agtcctcggc
     2941 ccggaggccc ggtctcggac ggtcatcgca gtcgccgtcg gcgcgggcct ggttgaggcg
     3001 gccagcatgc tgccctacct cggcgccgtg gggatgctca cctcctcggt cgcatccaca
     3061 ccggagcggc tggctgcgct ggccagctac gtcgtcgtca tgtgtctgcc ggcgctcatc
     3121 ctgctcgcgg ccagactgct gctgggcgca cgggtcgacg gcgcgcttgg caggctcgcc
     3181 cgctggctcg atcgacagtc gaggaacgcg atctggtggg tcgtcggcgt ggtcggattc
     3241 ctcatcgcga gagacgccgc ctcccgaatc tggccggggt cgatgggcta acaccctcgg
     3301 ccccacccat cgtgcgcgca acgcggcagg ccagcaggta tgccgcccgc gccgggtgcg
     3361 gccagcgacc ggcgggccct gcggttgtag gcgacgcagc atacccattt gtcgggggac
     3421 caccgcgtca gcgacactgg ggggtccagc cgggcgcccg tggcgtccgg ttcactgcgt
     3481 ctacggagtc tctcgtgctg tccgcctatg tccccctgtt ccgcgtccca ggcgccctgc
     3541 gattcatcgc cgggacggcg ctgtcgcgcg tcggcgggtc gatgttcggg gtggcgatca
     3601 tcgtcatgct cagcgagcgg cgggggagtt tcggtctcgc gggggcgatc tcagcggtcg
     3661 gagtcggcac gctcgccgtc gcggggccgt tcatcgggcg gctcatcgac aagcacggac
     3721 aacgtcggat cgccctgccc ttcatcgtgt cgtccgcgat cttcgggacc tcggcggcgg
     3781 tgctgtcgat cgtcaacgcg ccggtgtgga cgatctttgt ggcctatgcc ctgtctgcgg
     3841 tgttgcccga gcccggcccg atgtcgaggg cgcgctgggc gcacatctat cgggacgacg
     3901 cggaccaact gcacacggcg atgtccttcg agcaggtcgc cgacgagggg tcgttcgtca
     3961 tcgggccggt cgtcgccgtc ctcgtctcga cgctgtggtt cccggaggcc gggctgctca
     4021 tcgccgaact gctcttcact gtggggatgg tcgccttcct caccgcgacc gcgaccgagc
     4081 cgccagtcgt cgcccacgaa aaccggcccg cgggcctcgc gatccgctgg cccggtctga
     4141 tgctcgttgc gaccgcgttg acgatgacgg gggtcatctt cgggtccaac gaggtgatcg
     4201 ccgtcgcctt cgccgatgcg cgtggcaata ccggtttttc gagcgtcgtc ctcggcgggt
     4261 tcgccctcgg gtcgacgctc gccggcatcg tcttcggaag ccggaagttc aactcgacgc
     4321 tctcccggag gctgttgctc gcagcggccg ggatgtttgt gcttgaagcg ccggccttct
     4381 tcatgagtga cctctggccg ctcgctgtgg tcatgttcat cgcagggtcg gcgaccgcgc
     4441 ccatgctcat cacgagcctg tccctggccc aggtgctcgt gcccaaggcg atggtgaccg
     4501 aggggatggc cgtcgcgatc accggcatcc tcatcggcat ctcgggaggt gcggccgtgg
     4561 gcggctgggc gatcgaggcg tggggtgccc aacgcgcgta tgccgtgccg atcctcgcgg
     4621 gggcggccgc gctcgccatc atcgtggcta ggttccggca cgtccagcgt gccgaactca
     4681 ccgcgtccgc cgaggcctga cctcgccgcg cgagaacgcc tagacggcgt tcctcgcagc
     4741 gtggttggca gagaacgcaa cccggacgaa caggccgagc atcacgacca gaccggtggc
     4801 tgagacgacc gctaacgcca actctgcttg cggtgcgagg agccaggcca cgaggacagt
     4861 gacgagtgcg gcggctcccg tcacggccgc agtcatgagg gccggctgcg ctgccacatg
     4921 gcccgctttc catgccgaat ccgaagcaag cgtcgccgac gtcttgatgc cgatcatggc
     4981 gttgcggtcg atcgagccgc tcttggtcag cgcgaactgc aggagagccg ccgcagtgag
     5041 ggcaacgatg actgctgttg agacgacgat gacggcgttc atccacaact ccttgagaag
     5101 acgcgttcct catgggtgcc ttccccaaaa agtctctcac gatggggcct gcctatcgag
     5161 gcggacggag aaggctctcg cggtagccat tcgccccgta gaaatctgta cctcaggtac
     5221 gggctgcgtg cttgaggtac gccgttctca cagcccactc caggggacct gccggacggc
     5281 atcgccccga taaggtcgcc ctggcaggct acgcccggtc cgggccggcc tgaccgacct
     5341 gaccgacctg ggcgaccgcg aggaagagcg ggaagtcctg ctcgaccccg tcgaccggtt
     5401 tgcgcaggtg gtagacggtg cgcaggtgtg ggtccacgaa gcccccctcg atcagctgcg
     5461 cggccaatgc ctcgcggtcg aagccatggt gcccatggaa gtggctgtgt cccgcgtggt
     5521 aggcgccgcc atcggcgtcg aggtcggcga tgcacagata cccacccggg cgcagggccg
     5581 cccggaggga gcgcagggcg gccggc
//
LOCUS       DAOMZY010000246         5570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_13725_length_5570_cov_6.198549, whole genome shotgun sequence.
ACCESSION   DAOMZY010000246 DAOMZY010000000
VERSION     DAOMZY010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13725_length_5570_cov_6.198549"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="PLK64_12720"
     CDS             <1..1282
                     /locus_tag="PLK64_12720"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HPV80658.1"
                     /translation="VEYLNPGGSVKDRIALRMIEAAERSGELRPGGTIVEPTSGNTGV
                     GLALVAQRKGYRCVFVCPDKVSEDKRNVMKAYGAEVVVCPTAVAPEHPDSYYNVSDRL
                     VREIEGAWKPNQYANPEGPASHYATTGPEIWGDTDGRVTHFVAGVGTGGTISGTGHYL
                     KDQAAAVGRPGQVHVIGADPEGSVYSGGTGRPYLVEGVGEDFWPTAYDPAVVDEVVAV
                     TDADSFAMTRRLAREEGLLVGGSCGMAVVAALQIARDLPADAVVVVLLPDGGRGYLSK
                     VFADQWMNSYGFLREAGERTVGEILHAKSGALPALVHTHPSETIREAIDILREYGVSQ
                     MPVVKAEPPVVSGEVAGSVSERALLEALFTGAAHLSDSVERHMEPGLPLVGAGEPVSV
                     ARTELEHADAVLVVEDGKPVGVLTRADLLGFLVG"
     gene            complement(1304..1711)
                     /locus_tag="PLK64_12725"
     CDS             complement(1304..1711)
                     /locus_tag="PLK64_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80659.1"
                     /translation="MSPITVVASGELTAIASATTAAAASAPAYLPALLLTLVVEVPAL
                     LLLARLFARVSVPLALAGAVGVNLLTHPILYAASAGFSSWTQLLAAEALVAFVEGLAL
                     AWVWRVPLRESWPLVGAFAVNALSTLAGLALYR"
     assembly_gap    1781..1880
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1881..2213
                     /locus_tag="PLK64_12730"
     CDS             <1881..2213
                     /locus_tag="PLK64_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80660.1"
                     /translation="VSSVAWPAAPEAAMRWLVAFAFLQTMHYVVWVWFLPRAAPEATA
                     AFEARWPVASSRRVWGVGVALAVALGALLLTDYGQGRTVYSGLASYHAYLEFPVLLVL
                     LTRWRRSP"
     gene            complement(2546..3751)
                     /locus_tag="PLK64_12735"
     CDS             complement(2546..3751)
                     /locus_tag="PLK64_12735"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrophosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HPV80661.1"
                     /translation="MSREIRRIALLTAGGYAPCLSSAVGGLIERYSQVLPDAEIIGYL
                     HGYHGVLAGNYLVVDQETRDKAHLLHDFGGSPIGNSRVKLTNVKDLVKRGLVKEGENP
                     LHVAAERLKADGVDVLHTIGGDDTNTTAADLAAYLHEAGYELTVVGLPKTIDNDVVPI
                     RQSLGAYTAAEEASLFAQNILAENGSNPRMLIIHEVMGRGCGWLTGKAASNYQAWHAE
                     QEWVPSFGLSAERWAIHGVYVPEIHIDLDAEAARLTKVMDEVGCVNLFLSEGAGVPDI
                     VAAMEAAGQEVPRDPFGHVKLDMINPGQYFAKQFAEKLGAEKTMVQKSGYFSRSARAN
                     AQDLELIAAMCDKAVEAAMAGIPGVVGHDEERGDELRAIEFPRIAGHKAFDTSVDWFQ
                     DVLRTTGQA"
     gene            complement(3926..4075)
                     /locus_tag="PLK64_12740"
     CDS             complement(3926..4075)
                     /locus_tag="PLK64_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80662.1"
                     /translation="MGPGPYAAPPPGVPRKSNAGKTVAIVILTVIGGSALLMIVLAGV
                     CLSQL"
     gene            complement(4309..4629)
                     /locus_tag="PLK64_12745"
     CDS             complement(4309..4629)
                     /locus_tag="PLK64_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80663.1"
                     /translation="MTNPPVTPGIPGVPVAALPEATAPFDPLRLCIFATVALVGWALG
                     PFALLFFAGLGFTGYWKARRAGLTRSRCLLRDTRLVLTYLAVLAAAACVGIWFLLTRL
                     GRLT"
     gene            complement(4626..>5570)
                     /locus_tag="PLK64_12750"
     CDS             complement(4626..>5570)
                     /locus_tag="PLK64_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018770107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPV80664.1"
                     /translation="RHRERVEVYLQFDGLTPTGSIHHRGADLLRFKKAALERLTGAGV
                     FTTLTLTAALGVNDDQIGPVIQLALQTPFVGGVTIQPVFGSGRSAGIDPVDRLTHTGV
                     LARLGEQTDGQITWRDLTALPCSHPHCASVGYFLRDDSGQWRSLVSLIGPDRLKDYLD
                     LAPDEFANRFADQDIPARLRASVKDSLLGLLSEQSSVSHPDIGRIWQDICTTCDIGIG
                     TLTTLAASRLPGQAGRLRTMLAERVLRITVKPFMDINTMIEERLTQCCVHVGTVNPGD
                     GSHQCAPFCAVQAWAPLSRTRLSTAAGSLTRPPAEVSS"
BASE COUNT          804 a         1815 c         2003 g          848 t
ORIGIN      
        1 ggtcgaatac ctcaacccgg gtgggtccgt gaaagaccgg atcgccctgc ggatgatcga
       61 ggcagccgag cgctccgggg agttgcgccc cggcggcacc atcgtcgaac cgaccagcgg
      121 caacaccggg gtgggcctgg ccctcgtcgc ccaacgcaag ggttaccggt gcgtgttcgt
      181 ctgccccgac aaggtcagtg aggacaagcg caacgtcatg aaggcttacg gcgccgaggt
      241 cgtcgtctgc ccgacggccg tcgctccgga gcaccccgac tcctattaca acgtctccga
      301 ccggctggtc cgcgagatcg agggcgcctg gaagcccaac caatacgcca accccgaagg
      361 ccccgcgagc cactacgcga cgaccggccc ggagatctgg ggcgacaccg acgggcgggt
      421 cacccacttc gtcgccggtg tcggcacggg cgggacgatt tccggcaccg gtcactacct
      481 caaggaccag gccgcggcgg tcgggcgccc cggccaagtg cacgtcatcg gtgccgaccc
      541 ggagggctcg gtctactccg gcgggaccgg gcggccctac ctcgtcgagg gggtcggcga
      601 ggacttctgg cccacggcat acgaccccgc ggtcgttgac gaagtcgtgg cggtcaccga
      661 cgcggactcg ttcgcgatga cccggcgact ggcgcgcgag gagggcctgc tcgtcggagg
      721 gtcgtgcgga atggcggtcg tcgccgccct gcagatcgcc cgcgacctgc ccgccgacgc
      781 ggtcgtcgtc gtcctgctgc ccgatggtgg gcgcggctat ctctccaagg tcttcgccga
      841 ccagtggatg aactcctacg gcttcctgcg cgaggccggt gagcggaccg tcggcgaaat
      901 cctgcatgcc aagtcgggcg cgctgcccgc gctcgtccac acccacccga gcgagacgat
      961 ccgcgaggcc atcgacatcc tgcgcgaata cggcgtctcg cagatgccgg ttgtcaaggc
     1021 cgagccgccg gtcgtgtccg gtgaggtcgc cgggtcggtg agcgaacgcg ccctgttgga
     1081 agccctgttc acgggcgccg cgcacctgag cgactcggtc gagcggcaca tggagcccgg
     1141 cttgccgctg gtcggcgcag gcgagccggt cagcgtcgcc cgcacggagc tcgagcacgc
     1201 cgacgcggtg ctcgtcgtcg aggatggcaa gccggtcgga gtgctcactc gggccgacct
     1261 gctcggcttc ctcgtcggct gactccgctc gtcggagcgc gcgtcagcgg tagagcgcca
     1321 ggcccgcgag ggtcgacaag gcgttgaccg cgaatgcgcc taccagcggc caggattcgc
     1381 gaagcggcac ccgccagacc caggccagcg cgaggccctc aacgaacgcg acgagggcct
     1441 cggcggccag cagttgcgtc caggacgaga agccggcgga cgcggcatac agaatggggt
     1501 gcgtgagcag gttgaccccg accgcgccgg ccagcgcgag cggcaccgac actcgcgcaa
     1561 acagccgcgc gagcagcagc agtgcgggca cctcgacgac cagcgtgagc agcagcgccg
     1621 gcaggtatgc cggtgcgctg gctgccgcgg cggtggtggc gctcgcgatc gcagtgagtt
     1681 cgccgctggc gaccacggtg atcgggctca tctgcttcgg gatccttcac aacgtgctcg
     1741 aattgcgtta cgtcctgggg cgattcggtg atctgctgac nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn gtgtcgtcgg tcgcgtggcc ggccgcgccg gaggccgcga
     1921 tgcgttggct ggtcgccttc gcgttcctgc agacgatgca ctacgtcgtc tgggtgtggt
     1981 tcctgccgcg cgccgctccc gaggcgacgg cggccttcga ggcgcggtgg ccggtggcga
     2041 gctcgcggcg ggtgtggggc gtgggggtcg ccctggcggt ggctctcggc gcgctcctgc
     2101 tcaccgacta tggtcagggg cgaacggtct attcggggtt ggcgagctat cacgcctatc
     2161 ttgagttccc cgtgcttctc gtcctgctca cccgttggag gcgttcgccg tgagagtgtc
     2221 cctggtcact gacgtggcaa ccgcccccat ggtcgccggc ttggtcgtct tcctcgtcct
     2281 ggggttcgtc gtcgtggtcg gactggtcat cctggccgtc ctcctggtgc gccgcagccg
     2341 tcgctagcgt tgcgcccccc caccccgggg gatgaagccg cacgtgaagc cgcggcaggt
     2401 ggtgtgatca cgccacctgc cgcggcttca tgagcggccc cacgcccggc cgagcggcat
     2461 acctggtgcc cgcttgggcc ccgtcgctca ggctgtctct cgtgcctggc tccttggcgc
     2521 ttgcttcgct gcgcgccttc gtcgctcagg cttgaccagt ggtgcgcagc acgtcctgga
     2581 accagtcgac gctcgtgtcg aaggccttgt gcccggcgat ccgggggaac tcgatggcgc
     2641 gcaactcgtc gccgcgctcc tcgtcgtggc cgacgacacc cgggatgccg gccatggcgg
     2701 cctcgacggc cttgtcgcac atcgccgcga tgagctcgag gtcctgggcg ttggcccgcg
     2761 ccgaacggct gaagtagccc gacttctgga ccatcgtctt ctcggcaccg agcttctcgg
     2821 cgaactgctt ggcgaagtac tgaccggggt tgatcatgtc gagtttgacg tggccgaacg
     2881 gatcccgcgg gacctcctgg ccggctgcct ccatggccgc gacgatgtcg gggacgccgg
     2941 cgccctcgga caggaagagg ttgacgcagc cgacctcgtc catgaccttc gtcaggcggg
     3001 cggcctcagc gtcgaggtcg atgtggatct ccgggacgta gacgccgtgg atggcccacc
     3061 gctccgcgga caacccgaag ctcggcaccc actcctgctc ggcgtgccac gcctggtagt
     3121 tgctcgcggc cttgccggtc agccaaccgc agccccggcc catgacctcg tggatgatga
     3181 gcatccgcgg gttcgagccg ttctcggcga ggatgttctg cgcgaacagc gacgcttcct
     3241 cggcagccgt gtaggcaccg agcgactggc ggatcggcac gacgtcgttg tcgatcgtct
     3301 tgggcaggcc gaccaccgtc agctcgtacc cggcctcgtg caggtatgcc gcgaggtcgg
     3361 cggccgtcgt gttcgtgtcg tcgccgccga tggtgtgcag gacgtcgacg ccgtcagcct
     3421 tgagccgctc ggccgcgacg tgcagggggt tctcgccctc cttgaccagc ccacgcttga
     3481 ccaggtcctt gacattggtc agcttgaccc gactgttgcc gatcgggctg ccgccgaagt
     3541 cgtgcagcag gtgggccttg tcgcgggtct cctggtcaac gaccaggtag ttgccggcga
     3601 gcaccccgtg gtagccgtgc aggtagccga tgatctcggc gtccggcaac acctgcgaat
     3661 agcgctcgat gaggcctccg accgcggagg acagacacgg ggcatagccc ccggcggtga
     3721 gcagcgcgat gcggcggatc tcgcgagaca tgggcgggca ccttccaggc tgtgggcagt
     3781 cgcctccaac cctattgggg tatgccgccc gcccggcgtc gggtgcggct aacggcactg
     3841 gcgcgccccg ggtggcccgc tgcgcagacg ggtttcatcg tcggttcgtc gagtcggtct
     3901 gctcgaagca tctggagcga ggtgctcaga gctgggagag gcacacgccg gcgagaacga
     3961 tcatcaacaa ggcggacccg ccgataacgg tgaggatgac aatggcaacg gtcttgcccg
     4021 cgttgctctt gcgggggacg ccgggcggcg gcgcggcata cgggccgggt cccatcggcg
     4081 gggcaccgta cgggccgggt cccatcggcg gggcaccgta cgggccgggt cctatcggcg
     4141 gggcaccgta cgggccgggt cctatcggcg gggcaccgta cggaccaggt gccatgggcg
     4201 gcggcggcgc ggcatacgga ccaggcggga tgggcggcgg cgggggcgcc tgcgccggcg
     4261 ggaactgcgg gtcgaagggc gacgggccgg aatcaggcgg gggctgggtc atgtgagtct
     4321 cccgagacgg gtgagaagga accagatgcc gacacacgcg gcggcggcga ggacggcgag
     4381 gtaggtgagg accaagcggg tgtcgcgcag caggcagcga ctgcgggtga ggccggcccg
     4441 gcgggccttc cagtagccgg tgaaccccag ccccgcgaag aacagcagcg cgaacggccc
     4501 gagggcccaa ccgaccaatg ccaccgtggc gaagatgcac aaccgcaagg gatcgaaggg
     4561 cgccgttgcc tcgggcaggg cggccacagg cactcctggt atgccgggtg tcacgggtgg
     4621 gttggtcaac tcgacacctc cgcgggtggt cgggtgaggg aaccggctgc agtgctcagg
     4681 cgagtccggg acagcggggc ccaggcttgg accgcgcaga acggcgcaca ttgatgactg
     4741 ccgtcgcccg ggttgacggt gccgacgtgg acgcagcact gggtgaggcg ttcctcgatc
     4801 atcgtattga tgtccatgaa cggcttgacg gtgatccgca acacccgctc ggcgagcatg
     4861 gtgcgcaggc ggccggcctg gcccggtagg cgcgaggcgg ccagcgtcgt cagggtgccg
     4921 atcccgatgt cacaggtggt gcagatgtcc tgccagatcc gcccgatatc agggtgggag
     4981 acggacgact gctcgctgag caggccgagc aggctgtcct tgaccgaggc gcgcagccgc
     5041 gcggggatgt cctggtcggc gaaccggttg gcgaattcgt ccggagcgag gtcgagatag
     5101 tccttgagcc ggtccggacc gataagtgac acgagcgagc gccactgacc cgagtcgtcg
     5161 cgcaggaagt agccgaccga ggcgcagtgc gggtgcgaac agggcagggc ggtgagatcg
     5221 cgccaggtga tctgcccgtc ggtctgctcg ccgagccggg ccaggacgcc ggtgtgggtg
     5281 agtcggtcga ccgggtcgat gcccgcgctg cgtcccgagc cgaagaccgg ctggatggtc
     5341 acgccgccga cgaagggcgt ctgcagggcc aactggatga ccggaccgat ctggtcatcg
     5401 ttgacgccaa gcgccgccgt cagggtcagg gtcgtgaaca cgcccgcgcc ggtgaggcgc
     5461 tcgagcgccg ccttcttgaa ccgcagcaga tcggccccgc gatggtggat cgacccggtc
     5521 ggcgtgagcc cgtcaaactg gaggtagacc tcgacccgct ctcggtgacg
//
LOCUS       DAOMZY010000247         5558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_13757_length_5558_cov_4.904597, whole genome shotgun sequence.
ACCESSION   DAOMZY010000247 DAOMZY010000000
VERSION     DAOMZY010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13757_length_5558_cov_4.904597"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /gene="gap"
                     /locus_tag="PLK64_12755"
     CDS             <1..1000
                     /gene="gap"
                     /locus_tag="PLK64_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPV80665.1"
                     /translation="KVGINGFGRIGRNFYRAVVESGADIEVVAVNDLTDNKSLAHLLK
                     YDSILGRFPGEVTYDEASITANGKTFAAWAERDPAKLDWASVGADIVIESTGFFTDAT
                     KAKAHVDGGAKKVIISAPASNEDITIVMGVNDDKYDPALHTVISNASCTTNCLAPMAK
                     ALNDGLGIVKGLMTTIHAYTQDQNLQDAPHKDLRRARAAALNMVPTTTGAAKAVALVL
                     PELKGKLDGYAMRVPTPTGSATDLTFEAARETTVAEVNAIVKAAAESGPLKGYLVYTE
                     DPIVSKDIETDPASCIFDAPLTKVIGNQVKVLGWYDNEWGYSNRLVDLCALVGSKL"
     gene            1046..2248
                     /locus_tag="PLK64_12760"
     CDS             1046..2248
                     /locus_tag="PLK64_12760"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPV80666.1"
                     /translation="MKTIAGLGDLRGKRVLVRSDLNVPLDGDKNITDDGRIRASVPTI
                     KALTEAGARVVVCAHLGRPKGAPEEKYSLAPVVGRMSELLGQPVAFATDTVGESAQAV
                     VAGLGDGDVALLENIRFNAGETSKDEGERAAFAGQLAALADAYVSDGFGVVHRKQASV
                     YDIVRLLPSAMGGLVAAEVEVLKRLTEDPARPYAVVLGGAKVADKLAVIENLMKTADR
                     LLIGGGMAFTFLAAEGYEVGSSLLDKDNIETCKGYLARAKESGVEIILPVDVVAATAF
                     SADAEHDVVPADGIPADRMGLDIGPRSAELYASKVRDAKTVFWNGPMGAFEMAPYAAG
                     TAAVAKALVEATADGALTVVGGGDSAAAVRQLGFTDDQFGHISTGGGASLEYLEGKEL
                     PGIAILNG"
     gene            2271..3059
                     /gene="tpiA"
                     /locus_tag="PLK64_12765"
     CDS             2271..3059
                     /gene="tpiA"
                     /locus_tag="PLK64_12765"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPV80667.1"
                     /translation="MSRIPLLAGNWKMNQDHLQATHLVQKLDWTLRDGKHDFGAVEVA
                     VLPPFTDLRSVQTLVEGDKLSVRYGAQDLSVHDSGAYTGEISGAFLAKLGCTYVTIGH
                     SERREYHAETDAVVNDKVKAAYRHGVTPILCCGEGLDVRKAGGQVAHVVAQLTRDLEG
                     IPAEQARTIVIAYEPIWAIGTGEVATPEDAQEVCAAIRTLLAELYSGDLADGVRVLYG
                     GSVKSSNVAAIMAKEDVDGALVGGASLDAAEFAAICRFKDHVTG"
     assembly_gap    3073..3172
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3270..3518
                     /gene="secG"
                     /locus_tag="PLK64_12770"
     CDS             3270..3518
                     /gene="secG"
                     /locus_tag="PLK64_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPV80668.1"
                     /translation="MTVVKIALQALLVLSGLFLTLLILMHKGKGGGLSDMFGGGMSSS
                     LGGSTVAERNLDRFTIAVAILWFTAVVGLGLVERFVAA"
     gene            3534..3884
                     /locus_tag="PLK64_12775"
     CDS             3534..3884
                     /locus_tag="PLK64_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HPV80669.1"
                     /translation="MASGNAIRGSRVGAGPMGEAERGEAAPRVHISYWCTNGHETRPS
                     FAQEPGMVAPEHWDCPRCGLPAGQDRENPPAPPKVEPYKTHLAYVKERRSDADGQAIL
                     NEALGALRQRGLIQ"
     gene            complement(4003..4458)
                     /locus_tag="PLK64_12780"
     CDS             complement(4003..4458)
                     /locus_tag="PLK64_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013599858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HPV80670.1"
                     /translation="MITDPAVEFVGELLATGGTTTGFDVPESVVTSLGSGKRPKVLVT
                     VGGHSWQTSIAPMGGRFMLGVSAANRAAAGLEAGQTVPVTVVLDTAPRVIEVPSDLAE
                     ALAAAPDAAAGWARLSYSHQRAHVEALLAAKKPETRASRLTKTLAMLTA"
     gene            complement(4455..5204)
                     /gene="pgl"
                     /locus_tag="PLK64_12785"
     CDS             complement(4455..5204)
                     /gene="pgl"
                     /locus_tag="PLK64_12785"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HPV80671.1"
                     /translation="MSEHPTNAPLVVVHPDKQGVSDAAGARLLLALLDTLCVRDDAHV
                     VLTGGSLGSDMLASAGRSPLARLVDWSRVHLWWGDERYLPDGDPDRNETQNRAALLDH
                     LPLDPAKIHGMAGPDRSDSAEASAAAYATELRAHGGGLFDVVLLGMGPDGHVASLFPQ
                     HPAQLVTDTIAVAVHDSPKPPPDRVSLTFECLARSRQVWLLVAGADKAPAVCRALTPG
                     AQRWDVPAAGVRGLDATLWLVDVEAASELPS"
     gene            complement(5201..>5558)
                     /locus_tag="PLK64_12790"
     CDS             complement(5201..>5558)
                     /locus_tag="PLK64_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80672.1"
                     /translation="RISLRRRGDAECLADELRRLDADETYADVLRKGLPALGTTRGAS
                     RSAAVSESKREAERERRNHAAQLRTAAAEAGSLPSTVDTTTPAKASGSTPTKPRVTRS
                     TSATARTGRTTKEQPS"
BASE COUNT          865 a         1906 c         1870 g          817 t
ORIGIN      
        1 caaggtaggc atcaacggct tcggccgcat cggccgtaac ttctaccggg ccgttgtcga
       61 gtccggcgcc gacatcgagg tcgtggctgt caacgacctg actgacaaca agagcctcgc
      121 gcacctgttg aagtacgact cgatcctcgg gcgtttcccg ggcgaggtca cctacgacga
      181 ggcctcgatc acggccaacg gcaagacctt cgccgcctgg gccgagcgcg acccggccaa
      241 gctcgactgg gccagcgtcg gcgctgacat cgtcatcgag tccaccggct tcttcaccga
      301 cgcgaccaag gccaaggccc acgtcgacgg cggcgcgaag aaggtcatca tctcggcgcc
      361 ggcgtccaac gaggacatca ccatcgtcat gggtgtcaac gacgacaagt acgacccggc
      421 cctgcacacg gtcatctcga acgcgtcgtg cacgaccaac tgcctggccc ccatggccaa
      481 ggcgctcaac gacggcctcg gcatcgtcaa gggcctgatg acgacgatcc acgcctacac
      541 gcaggaccag aacctgcagg acgccccgca caaggacctg cgtcgcgccc gcgccgccgc
      601 tctcaacatg gtcccgacga cgaccggtgc cgccaaggcc gtggcgctcg tcctgcccga
      661 gctcaagggc aagctcgacg gctacgccat gcgcgtcccg acaccgaccg gctcggcaac
      721 cgacctcacc ttcgaggccg cccgcgagac gaccgtcgcc gaggtcaacg ccatcgtcaa
      781 ggctgcggcc gagtccggcc cgctcaaggg ttacctcgtc tacaccgagg acccgatcgt
      841 gtccaaggac atcgagaccg acccggcctc gtgcatcttc gacgccccgc tgaccaaggt
      901 catcggcaac caggtcaagg tgctcggctg gtacgacaac gagtggggct acagcaaccg
      961 cctcgtggac ctctgcgccc tcgtcgggtc gaagctctga cccgctagtc ccaccagaga
     1021 caccacactc caggaggttg gcaccgtgaa gaccattgcc ggattgggcg acctgcgcgg
     1081 caagcgcgtt ctcgtccgct cggatctcaa cgttcccctc gacggtgaca agaacatcac
     1141 cgacgacggc cggatccgcg cctcggtgcc gaccatcaag gccctgaccg aggcgggcgc
     1201 acgcgttgtc gtgtgcgccc acctcgggcg gcccaagggt gcgcccgaag agaagtactc
     1261 cctcgctccg gtcgtcggcc ggatgagtga gctgctcggc cagccggtcg cctttgcgac
     1321 cgacaccgtc ggcgagtcgg cccaggccgt cgtcgcgggt ctgggtgacg gcgacgtcgc
     1381 cctgctggag aacatccgct tcaacgccgg cgagacgagc aaggacgagg gggagcgcgc
     1441 cgcgttcgcc ggtcagctcg ccgccctcgc cgacgcctac gtctccgacg gtttcggtgt
     1501 cgtgcaccgg aagcaggcgt cggtctatga catcgtgcgg ttgctgccct cggccatggg
     1561 tggtctcgtc gcggccgagg ttgaggtgct caagcgcctc accgaggacc cggcccgtcc
     1621 gtatgccgtg gtgctcggcg gcgcgaaggt cgccgacaag cttgcggtca tcgagaacct
     1681 catgaagacg gccgatcggc tgctcatcgg tggcggcatg gccttcacct tcctcgccgc
     1741 cgagggctac gaagtgggtt ccagcctgct cgacaaggac aacatcgaga cctgcaaggg
     1801 ttacctcgcc cgggccaagg agtccggcgt cgagatcatc ctgccggtcg acgtcgtcgc
     1861 cgcgaccgcg ttctcggccg acgccgagca cgacgtggtg ccggcggacg gcataccggc
     1921 tgaccggatg gggctcgaca tcggtccccg ctccgcggag ctctacgcga gcaaggtgcg
     1981 cgacgcgaag acggtcttct ggaacggccc gatgggcgcg ttcgagatgg caccgtatgc
     2041 cgcgggcacg gctgccgtcg cgaaggcgct cgtcgaggct acggccgacg gggcactgac
     2101 cgtcgtcggt ggcggcgact ccgccgcagc cgttcgccag ctgggcttca ccgacgacca
     2161 gttcggtcac atctccaccg gtggcggcgc ctccctggaa tacctcgagg gcaaggaact
     2221 gcccggcatc gccatcctca acggctgacc acgaaggagc cgcaccactt atgagccgca
     2281 ttccgctgct tgccggtaac tggaagatga accaggacca cctgcaggcg acccacctgg
     2341 tccagaagct cgactggacg cttcgcgacg gcaagcacga cttcggtgcg gtcgaggtcg
     2401 cggtcctgcc gcccttcacc gacctgcgct cggtgcagac cctcgtcgag ggcgacaagt
     2461 tgtccgtgcg ctacggcgcc caggacctgt ctgtccacga ctcgggcgcc tacaccgggg
     2521 agatctccgg ggcgttcctc gccaagctcg gctgcaccta cgtgacgatc ggccacagcg
     2581 agcggcgcga ataccacgcc gagaccgacg ccgtcgtcaa cgacaaggtc aaggccgcct
     2641 accgccacgg cgtcaccccg atcctctgct gcggcgaggg cctggacgtc cgcaaggccg
     2701 gtggccaggt cgcccacgtc gtcgctcagt tgacccgcga cctcgaaggc atccccgccg
     2761 agcaggcccg cacgatcgtc atcgcctatg agcccatctg ggccatcggc accggcgagg
     2821 tggccacgcc cgaggacgct caggaggtgt gcgcggcgat ccgcaccctg ctcgccgagc
     2881 tctactccgg cgacctggct gacggcgtcc gcgtgctcta cggcggttcg gtcaagtcct
     2941 cgaacgtcgc ggccatcatg gccaaggagg acgtcgacgg cgccctcgtc ggcggcgcct
     3001 cgctcgacgc cgccgagttc gccgcgatct gccggttcaa ggaccacgtc accggctgac
     3061 ctcgcggtca ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccggcgtg
     3181 gggccgttcg ggatctgggc ggtctacggg tattctgacc ctcggcccct cggggcactc
     3241 cgaccgaccc gaaagtgggc gcgcaactcg tgaccgtggt gaagatcgcc ttgcaggcgt
     3301 tgctggtgct ctccggcctg ttcctgaccc tgctcatcct catgcacaag ggcaagggtg
     3361 ggggcctgtc ggacatgttc ggcggcggca tgtccagctc actcggcggg tcgaccgtgg
     3421 ccgagcgcaa ccttgaccgg ttcacgatcg cggtggccat cttgtggttc acggcagtcg
     3481 tcgggctggg cctggtcgag cgtttcgtcg cggcctgaga cgggggaacg acaatggcga
     3541 gtggaaatgc gatccgtggc agccgtgttg gcgccggacc catgggcgag gccgagcgcg
     3601 gcgaggctgc tccccgcgtg cacatctcct attggtgcac gaacggccac gaaacccggc
     3661 cgtcgttcgc gcaggagccc ggcatggtgg cgcccgagca ctgggactgc ccccgctgtg
     3721 ggttgcctgc tggccaggac cgcgagaacc ctccggctcc gcccaaggtg gagccctaca
     3781 agacgcacct ggcctatgtg aaggagcggc gctccgacgc cgacggccag gccatcctca
     3841 acgaggcgct gggcgccctg cgtcagcgcg gcctgatcca gtaggtcagc ccgatcgcag
     3901 atccccggcg gatgtgagga tccggggtca atccgggcct gcctgatcgc agatccccgg
     3961 cgggtgtggg gattttgggg gagcaggggc cttcacctgc ggtcaggcgg tgagcatcgc
     4021 caaggtcttg gtcagccggg aggcccgagt ctctggcttc ttcgcagcca acaacgcctc
     4081 aacgtgggcg cgctggtgcg agtagctgag ccgggcccaa ccggcagcgg cgtcgggtgc
     4141 ggccgccaac gcctcagcca gatcgctcgg cacctcgatc accctcggcg cggtgtcgag
     4201 cacgaccgtc acggggaccg tctgaccagc ctcaaggccc gccgcggccc ggttggcggc
     4261 gctgacaccc agcatgaagc gcccgcccat cggggcgatg ctcgtctgcc acgagtgccc
     4321 acccacggtc acgagcacct tgggccgctt gcccgatccg agggaagtga cgaccgactc
     4381 cggaacgtcg aagcccgtcg tcgtcccgcc cgtggccaac aactcgccga cgaactccac
     4441 agcggggtcc gtgatcatga ggggagttcc gaggcggcct cgacatcaac gagccacaag
     4501 gtggcatcca agccacgcac accggccgca gggacgtccc agcgttgcgc gccgggggtg
     4561 agggcacggc atacggcggg ggccttgtcg gcgccagcga cgaggagcca cacctggcga
     4621 ctgcgggcga ggcactcgaa ggtgagggac acccggtccg gcggcggttt gggggagtcg
     4681 tggaccgcga cggcgatcgt gtcagtcacc aactgggccg ggtgctgcgg gaagagcgag
     4741 gcgacgtgcc cgtccggtcc cattccgagc aggacgacgt cgaacaggcc gccgccgtgg
     4801 gcgcgcaact ccgtcgcgta tgccgcggcg ctggcctccg ccgaatccga acgatccggg
     4861 ccggccatgc cgtggatctt cgccgggtcc aacggcaggt gatcgagcag ggcggcgcgg
     4921 ttctgggtct cgttgcggtc gggatcgccg tcgggaaggt agcgctcgtc gccccaccac
     4981 aggtggacgc gggaccagtc gacgagcctg gccaacggcg agcggccagc tgacgcgagc
     5041 atgtcggagc cgaggctgcc gccggtgagc acaacgtgag cgtcgtccct gacacagagg
     5101 gtgtcgagca gggccagcag cagtcgtgcc ccggcggcgt ccgagacgcc ctgcttgtcg
     5161 gggtggacga cgacgagggg tgcgttggtg gggtgctcgc tcatgacggc tgctccttcg
     5221 tggtccggcc ggtgcgcgcg gtggctgagg tcgaccgggt gacgcggggc ttcgtcgggg
     5281 tggacccgga agccttggcg ggggtggtcg tgtcgacggt tgagggcaag gagccggcct
     5341 cggcggcggc cgtccgaagt tgcgccgcat ggttgcgccg ctctcgttcg gcctctcgct
     5401 tcgactcgct caccgcggcc gagcggctgg ctcccctcgt ggtgcccaag gcgggcagac
     5461 ccttgcgcaa cacgtcggcg taggtctcgt cggcgtccag gcggcgcaat tcgtcggcca
     5521 ggcattcggc gtcgccgcgc cggcgcaggc tgatccga
//
LOCUS       DAOMZY010000248         5530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_13848_length_5530_cov_5.664292, whole genome shotgun sequence.
ACCESSION   DAOMZY010000248 DAOMZY010000000
VERSION     DAOMZY010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13848_length_5530_cov_5.664292"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /gene="nuoF"
                     /locus_tag="PLK64_12795"
     CDS             <1..1062
                     /gene="nuoF"
                     /locus_tag="PLK64_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPV80673.1"
                     /translation="ESEPGTCKDIPLMMANPHALIEGAAISQYAMGSSHAFIYLRGEV
                     VHVYRRLMRAIEEATAAGYLGSNVFGSGITLQVTAHAGAGAYICGEETALLDSLEGRR
                     GQPRLKPPFPGAAGLYARPTSVNNVETIASVPGILLNGATWFKSMGTEKSTGFGIFSL
                     SGHVANPGQFEAPLGITMRQLIDLAGGIRKGHQLKFWTPGGSSTPIFTEAHLDIPLDF
                     DSVAAAGSMLGTRALQVFDETVSVVRAVARWTDFYAHESCGKCTPCREGTWWMRQIME
                     RLEHGQGLPGDIEKLDDICDNILGRSFCALGDAATSPITSAIKHFRGEFEAGMHTPAA
                     ELFPPEKSVLFQVKEAAKA"
     gene            1059..3500
                     /locus_tag="PLK64_12800"
     CDS             1059..3500
                     /locus_tag="PLK64_12800"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HPV80674.1"
                     /translation="MTVPTSPASAAGQAPPPDAVTLTIDGVTVSVPKGTLIIRAAEQA
                     GIEIPRFCDHPGLDPVGACRQCLVDVAAPRPDGTLAPFPKPQASCTMTVSPGMVVNTQ
                     HTSPVADKAQHGVMELLLINHPLDCPVCDKGGECPLQNQAMSNGRAQTRFDDVKRTFT
                     KPINISTEVLLDRERCIVCQRCTRFSDQVAGDPFINLVERGAMQQIGIYEEQPFESYF
                     SGNTVQICPVGALTGAAYRFRSRPFDLVSTPSTCEHCASGCAIRTDHRRGVVLRRMAI
                     DDPEVNGEWSCDKGRWAFHSATLGDRLELPLLRQADGSMRAVGWPEALEAAAAGLSAA
                     KGAGVLVGGRVSAEDAYAYGKFARVVLGTNDVDFRARPHSAAETAFLAEHVVATGPAG
                     GAVTYADLSEAKAVVLVGFEPEEESPIVFLRLRRAVRKARTAVYAVAPFASHGLEKLS
                     GTLLPTVPGAEAAALADPSLAAALAGGGIILVGERMATSPGALAAAVSLAASTGARLA
                     WIPRRAGERGALEAGALGALLPGGRPVTDAVARGEVAAVWEVDSLPSAPGRDTAGIVA
                     AAASGALGALVVGGVDPADLALPQVADALAKAFVISLEVRESAVTAVADVVLPVAPHA
                     EKSGSFVNWEGRVRPFEAALSTNAMSDHRVLDMLAAELGFFMETRTHEQINDQFEALG
                     PWTGARAAANAAAAGSGATAYGSGDGDVVLATWPLLLDRGRMQDGEPFLAGTAHASVA
                     RVGATLAGRLGVTDGDAVTVSSGSGALSVPVAVTDGMVDGVVWLPTNSAGCAVRSTLG
                     VDAGARVTVSKGGAQ"
     gene            3497..4858
                     /gene="nuoH"
                     /locus_tag="PLK64_12805"
     CDS             3497..4858
                     /gene="nuoH"
                     /locus_tag="PLK64_12805"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HPV80675.1"
                     /translation="MITSTLVPTIERPLADFSDTPLWLSLVKALFIFVYLLISTLLVI
                     WFERRIIGRMQQRPGPNRNGPFGLLQTLADGMKSMFKEDNAPAAADKFIFTLAPLIVA
                     TMCFVSFGIMPLGGDVVMWGHRTPLQLTDAPAAVLLVLAVASVGVYGIVLAGWSSGST
                     YPLLGGLRSSAQVISYEIAMGLSLVAVFIMSGSMSTSAIVDAQSSTWFVISAIPSFLV
                     YVVTMVGETNRLPFDLAEGEGEIVGGFHTEYGSMRFAMFFLAEYINMFTVSALATTMF
                     LGGWQAPPFIAAIGDGMLNAGWWGLLWFTIKVWSFMFFFVWLRGSLPRVRYDQFMRLG
                     WKLLIPLTLAWVVLVAFMRAAALGWFGTSTVISPFGRGLSAASLVLIGLVLVGLFAIG
                     FLLDRTSSSHAAAPVEQPDEIDPFAGGFPVPPLPGQTLREPERGIPAVAASTLTAKEE
                     SRG"
     gene            4974..>5530
                     /gene="nuoI"
                     /locus_tag="PLK64_12810"
     CDS             4974..>5530
                     /gene="nuoI"
                     /locus_tag="PLK64_12810"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HPV80676.1"
                     /translation="MGGFGVTWATMFRKVLTEEYPEEKRPTAPRYHGRHQLNRHPDGL
                     EKCIGCELCAWACPADAILVEGGNNDDTPADAGGTGRFSPGERFGRVYQINYLRCIFC
                     GLCIEACPTRALTMTNAYELADNNRADLIFTKEQLLAPLQQGMLAPPHAMVDGMEERD
                     YYLGKVNGSTEEQRVSAAAQAANAA"
BASE COUNT          761 a         1987 c         1842 g          940 t
ORIGIN      
        1 gagtccgagc cgggcacctg caaggacatc ccgctcatga tggccaaccc gcacgccctc
       61 atcgagggcg cggcgatcag ccagtatgcg atggggtcga gccacgcgtt catctatctg
      121 cgcggcgagg tcgtccacgt ctaccggcgg ctcatgcggg ccatcgagga ggcgaccgca
      181 gcgggttacc tcgggtccaa cgtctttggc tcaggcatca cccttcaggt cacggcccac
      241 gccggtgccg gcgcctacat ctgtggcgaa gagacggccc tgctcgactc ccttgagggt
      301 cgccgcggcc aaccgcggct caagcccccg ttccccggcg cggcgggcct ctacgcccgc
      361 ccgacctcgg tcaacaacgt cgagaccatc gcctcggtgc cgggcatcct gctcaacggc
      421 gcgacctggt tcaagtcgat gggcaccgag aagtcgaccg gcttcggcat cttcagcctc
      481 tcggggcacg tcgccaaccc cggccagttc gaggcgccgc tgggcatcac catgcgccag
      541 ctcatcgacc tcgccggcgg gatccgcaag ggtcaccagc tgaagttctg gacgccgggt
      601 gggtcctcga ccccgatctt caccgaagct cacctggaca tcccgctcga cttcgactcg
      661 gtggctgccg ccgggtcgat gctcggcacg cgggcgctgc aggtgttcga cgagaccgtc
      721 tcggtcgtcc gggcagtcgc ccgctggacc gacttctacg cccacgagtc ctgcggcaag
      781 tgcacgccct gccgtgaggg cacgtggtgg atgcgccaga tcatggagcg cctcgagcat
      841 ggccagggtc tgcccggcga tatcgagaag ctcgacgaca tctgcgacaa catcttgggc
      901 cgctcgttct gcgcgctcgg cgacgcggcc accagcccga tcacctccgc gatcaagcac
      961 ttccggggag agttcgaggc cggcatgcat accccggccg ccgagttgtt cccgccggag
     1021 aagtcggtcc tgttccaggt caaggaggcc gcgaaggcat gaccgtcccg accagccccg
     1081 cttctgccgc cgggcaggcg ccgccgcccg acgcggtaac cctgacgatc gacggcgtga
     1141 ccgtgagcgt gcccaagggc acgctcatca tccgcgccgc cgaacaggcc ggcatcgaga
     1201 tccctcggtt ctgcgaccac cccggcctcg acccggtggg cgcctgccgc cagtgcctcg
     1261 tcgacgtcgc ggccccgcgc cccgacggca cgctggcgcc gttccccaag ccgcaggcct
     1321 cgtgcaccat gacggtcagc cccggcatgg tcgtcaacac ccagcacacc tcgccggtgg
     1381 ccgacaaggc tcagcacggg gtcatggagt tgctgctcat caaccacccg ctggattgcc
     1441 ccgtctgcga caagggcggc gagtgccccc tgcagaacca ggcgatgtcc aacggccgcg
     1501 cccagactcg gttcgacgac gtcaagcgca ccttcaccaa gccgatcaac atctccaccg
     1561 aggtgttgct cgaccgcgag cgctgcatcg tgtgtcagcg ctgcacgcgc ttctccgacc
     1621 aggtcgccgg tgacccgttc atcaacctcg tcgagcgcgg ggcgatgcag cagatcggca
     1681 tctacgaaga gcagccgttc gagtcctact tctccggtaa caccgtccag atctgccccg
     1741 tcggggcgtt gacgggcgcg gcataccggt tccgctcgcg gcccttcgac ctcgtgtcga
     1801 ccccgagcac ctgcgagcac tgcgcgtcgg gctgtgcgat ccgcaccgac caccgtcgcg
     1861 gcgtcgtgct gcgccggatg gcgatcgacg accccgaggt caacggcgag tggtcctgtg
     1921 acaagggccg ctgggcgttc cactcggcga cgctcggcga ccggctggag ttgccgttgc
     1981 tgcgccaggc cgacggctcg atgcgtgcgg tcggctggcc cgaggccctc gaagcggccg
     2041 ctgccggcct gagcgccgcc aagggtgccg gggtgctcgt cgggggccgg gtgtccgccg
     2101 aggacgccta cgcctacggc aagttcgccc gcgtggtgct gggcaccaac gacgtcgact
     2161 tccgggcgcg tccgcactct gcggccgaga cggccttcct ggccgagcac gtcgtcgcga
     2221 ccggccccgc cggtggcgcg gtgacctatg ccgacctctc ggaggccaag gccgtcgtcc
     2281 tcgtcgggtt tgagcccgaa gaggagtcgc cgatcgtctt cctgcggctg cggcgcgcgg
     2341 tccgcaaggc caggacggcg gtgtatgccg tggccccctt cgcctcgcac ggtctggaga
     2401 agctctccgg gaccctcctg ccgaccgtcc ccggagccga ggcggccgcg ctggccgacc
     2461 cgagtttggc tgcggctctc gcgggcgggg gcatcatcct cgtcggcgag cggatggcca
     2521 cctcgcccgg cgcgctggcc gctgcggtct ccctggccgc ctcgaccgga gcccgcctgg
     2581 cctggatccc gcgtcgcgcc ggtgagcgcg gtgctcttga ggccggtgcg ctcggcgccc
     2641 tgcttcctgg cggtcgcccg gtgaccgacg cagttgctcg cggtgaggtt gccgcggtgt
     2701 gggaggtcga ctccctgccg agtgcccctg gtcgcgacac cgctgggatc gttgctgccg
     2761 ccgccagcgg tgccctcgga gcgttggttg tcggtggtgt cgacccggcc gacctcgcgc
     2821 tgccgcaggt cgccgacgcg ctcgccaagg ccttcgtcat cagcctcgag gtgcgtgagt
     2881 ccgcagtgac cgccgtcgcc gacgtggtgc tgccggttgc cccacacgct gagaagtccg
     2941 gcagtttcgt caactgggag ggccgggtcc ggcccttcga ggcagcgctg tcgaccaacg
     3001 cgatgagcga tcaccgcgtg ttggacatgc tcgccgccga gttgggcttc ttcatggaga
     3061 cccgcaccca cgagcagatc aacgaccagt tcgaggccct cggtccgtgg accggtgccc
     3121 gggccgcggc gaacgccgcg gcggccggca gcggcgcgac ggcatacgga tcgggcgacg
     3181 gtgacgtcgt tctggcgacc tggccgctgt tgctcgaccg cggccggatg caggacggcg
     3241 agccgttcct ggctggcacc gcgcatgcga gcgtggctcg ggtcggtgcc accctggcgg
     3301 ggcgcctcgg tgtcaccgac ggtgacgcgg tcacggtctc cagcggcagt ggcgcgctca
     3361 gcgtgcccgt cgcggtgacc gacggcatgg tcgacggcgt ggtctggctg ccgaccaact
     3421 ccgcgggctg cgcggtccgc tccaccctcg gcgtcgatgc cggcgctcga gtcacggtgt
     3481 cgaagggagg tgcccagtga tcacgtcgac cctcgtcccg acgatcgaac ggcctctcgc
     3541 cgatttcagc gacacgccgc tgtggctgtc gctggtcaag gcgctgttca tcttcgtcta
     3601 cctgctcatc tcgacactgt tggtcatctg gttcgagcgg cgcatcatcg gccggatgca
     3661 gcagcgtccg ggccccaacc gcaacggccc gttcggtctg ttgcagacgc tcgccgacgg
     3721 tatgaagtcg atgttcaagg aagacaacgc gccggcggcc gccgacaagt tcatcttcac
     3781 cttggcaccg ctgatcgtcg cgaccatgtg tttcgtctcg ttcgggatca tgccgttggg
     3841 tggggacgtc gtcatgtggg ggcaccgcac cccgctgcag ttgaccgacg ctcccgcggc
     3901 cgtgctgctg gtgcttgccg tcgcgtcggt gggtgtctac ggcatcgtcc tggccggttg
     3961 gtcgtccggc tcgacctacc cgctgctcgg tgggctgcgc tcgtcggccc aggtcatctc
     4021 ctatgagatc gccatgggcc tgtcgctcgt cgccgtcttc atcatgagcg gatcgatgtc
     4081 gacctctgcc atcgtcgacg cccagagcag cacgtggttc gtcatctcgg cgatcccgtc
     4141 gttcctcgtc tatgtcgtga cgatggtcgg cgagaccaac cggttgccct tcgacctggc
     4201 cgaaggcgaa ggcgagatcg tcggtggctt ccacaccgaa tacggctcga tgcgcttcgc
     4261 gatgttcttc ctcgccgagt acatcaacat gttcacggtg tcggccctcg ccacgacgat
     4321 gttcctcggc ggctggcaag cgccgccgtt catcgccgcg atcggtgacg gcatgctcaa
     4381 cgccggctgg tggggcctgc tctggttcac catcaaggtc tggtcgttca tgttcttctt
     4441 cgtctggctg cgtggctcgc tgccccgcgt gcgttacgac cagttcatgc ggctgggctg
     4501 gaagttgctc atcccgctca ccctggcgtg ggtcgttctc gtcgcgttca tgcgcgccgc
     4561 cgccctcggc tggttcggca cctcgacggt gatcagcccc ttcggtcgcg ggctctcggc
     4621 cgcctcactg gtgctcatcg gcctggtcct tgtcgggttg ttcgcgatcg gcttcctgct
     4681 cgatcggaca tcctcgtcgc acgccgccgc accggtcgag caacccgatg agatcgaccc
     4741 gttcgcgggt ggtttccctg tgccaccgct gccggggcag accctgcgcg agcccgagcg
     4801 cggcatacct gccgttgccg cttcgaccct caccgcgaag gaggagagcc gtggctgaca
     4861 acctgccggc gaagccggac accagcggcg caaacctgcc ctcgaccacg catgccggct
     4921 ccgacgagcc caccccccgc gcgggctggc tcggcgaggc gcttccggcg tcgattggtg
     4981 gcttcggggt cacctgggcg accatgttcc gcaaggtcct caccgaggag taccccgagg
     5041 agaagcgccc cacggcgcct cgctaccacg gtcgccacca gctcaaccgg caccccgacg
     5101 ggctggagaa gtgcatcggc tgcgagctgt gtgcctgggc ctgcccggcc gacgcgatcc
     5161 tcgtcgaagg cgggaacaac gacgacacgc cggccgacgc gggcggcacc ggtcggttct
     5221 cacccggtga gcgtttcggc cgcgtctacc agatcaacta tctgcggtgc attttctgcg
     5281 ggttgtgcat cgaggcgtgc ccgacgcgag cgctgacgat gaccaacgcc tatgagttgg
     5341 ccgacaacaa ccgggccgac ctcatcttca ccaaggagca actgctcgct ccgctccagc
     5401 agggcatgct cgcaccgccg cacgcgatgg tcgacggcat ggaagagcgc gactactacc
     5461 tcggcaaggt caacgggtcc acggaggagc agcgcgtgtc ggctgctgcc caggcggcta
     5521 acgcggccga
//
LOCUS       DAOMZY010000249         5446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_14095_length_5446_cov_5.832128, whole genome shotgun sequence.
ACCESSION   DAOMZY010000249 DAOMZY010000000
VERSION     DAOMZY010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14095_length_5446_cov_5.832128"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /gene="cydB"
                     /locus_tag="PLK64_12815"
     CDS             complement(<1..592)
                     /gene="cydB"
                     /locus_tag="PLK64_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HPV80677.1"
                     /translation="MSDLSTLQIVWFVLIAVLWIGYLALEGFDFGVGMLLPILGRSHQ
                     KGAAGAADTEARKRVMLNTIGPVWDGNEVWVLTAGGAMFAAFPQWYATLFSGFYLALA
                     LILVALIVRGMGLDYRHKVDSDTWRKRWDLAITTGSFLAALLWGVGLTNIVYGSPIRP
                     NPDVKGDYFFDGNLFTLLGVVPLIGGLTFVGLFLTHG"
     gene            complement(609..2042)
                     /locus_tag="PLK64_12820"
     CDS             complement(609..2042)
                     /locus_tag="PLK64_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HPV80678.1"
                     /translation="MDVLDLARWQFAITTVYHFLFVPITIGVTALIAIMQTFWHRTGD
                     DHWLRLTKFFGKLFMINFALGLVTGIVQEFQFGMNWSLYSRFVGDIFGAPLAFEALLA
                     FFLESTFLGLWIFGWDRLPKKIHLASIWLVHIGTVFSAFFILAANSFMQNPVGYTYNA
                     QTNRAELDDFAAVLFNKVQLVTFPHVVLGAYMTAGAFVMGIALYHLRKQALDKGPDIS
                     MYRKAVRFGAIVALVASIGVLVSGDVQGKVMTEVQPMKMAAAEGLYETRSGAPFSILT
                     IGSLDGTAARPILEVPGLLSFLGTGSFSGEIQGINDLQAADKAEFGNNPQTASASYVP
                     VIWMTYWNFRLMMGAGFLTMLFAAMALWKTRGQGVVTSGMWKHIAIWTPLLPVLANSF
                     GWIFTEVGRQPWLVNGLMTTETGVSTSVSATSVWISMIIYTLLYGALAVVELRLLLRY
                     IQPGAVPVEAPKDPADRADDEPFVFAY"
     gene            complement(2241..3260)
                     /gene="galE"
                     /locus_tag="PLK64_12825"
     CDS             complement(2241..3260)
                     /gene="galE"
                     /locus_tag="PLK64_12825"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPV80679.1"
                     /translation="MRVLVSGGAGYIGSHTVVQLAAAGHDVVIVDSFANAKPTVVGRL
                     ESLTGTHLEVHGFDLTDKDKTERLFAAEKFDAVIHFAGLKAVGESVQIPLDYYVNNLE
                     STFSLLQAMRRHGCDLLVFSSSATVYGEVAPVPYQEDYEPLSASSPYGRTKVMIEHVL
                     RDVAAIDERLKVAILRYFNPVGAHPSGTIGEDPQGIPNNLMPFVAQVAVGRRERLQVF
                     GGDYPTADGTAERDYIHVEDLAAGHVAALQWIAGHDRKISTWNLGGGIGTSVLQVIAA
                     FEKASGRPIPYDIVGRRAGDLPAYWADPSRAHEELRWRAERSIDDMCADTWRWQSANP
                     GGYPE"
     gene            3778..4095
                     /locus_tag="PLK64_12830"
     CDS             3778..4095
                     /locus_tag="PLK64_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80680.1"
                     /translation="MSAAGRHVLGLAEREDVTYEFRSVMAWLAHREDLPSKWQHSMSS
                     GALITATDTQLIDLKLRMEALVADWSEACIRDQNETPDKDRWPVRVIARVFPATLGDV
                     AGA"
     gene            complement(4506..4727)
                     /locus_tag="PLK64_12835"
     CDS             complement(4506..4727)
                     /locus_tag="PLK64_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HPV80681.1"
                     /translation="MPDVLIPNVPAADLARLDAQAAQLGISLDEHLRRRLHQEACRLA
                     GPVKTADLQAVTDRIADLSDVEVMRAAWS"
     gene            4863..>5446
                     /locus_tag="PLK64_12840"
     CDS             4863..>5446
                     /locus_tag="PLK64_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80682.1"
                     /translation="MQDLEMDLLAPTFTTKAALALGVHPRDLYAWRDGGLLIELSRGV
                     FRKVDAPPATYPDLLAVTMRAPTSIVCCVSAAAVHELTDELPPAVQLAVPNGSHPPHI
                     DYPPTQVFRFASATFALGLSEVEAAPGEWVRIYDAARTVVDLMRLRHRFGETLAHTAL
                     NRSLTSGAARPADVLHCAQVLGVAGPVRLALDIA"
BASE COUNT          966 a         1704 c         1857 g          919 t
ORIGIN      
        1 cgccgtgggt gaggaacagc ccgacgaagg tgagcccacc gatgaggggc acgacgccca
       61 gcagggtgaa caggttgccg tcgaagaagt agtcaccctt gacgtccggg ttgggccgga
      121 tcgggctgcc gtagacgatg ttcgtcaggc cgacgcccca cagcaatgcg gcgaggaacg
      181 acccggtggt gatggcgagg tcccagcgct tgcgccacgt gtcggagtcg accttgtggc
      241 ggtagtccag gcccatgccg cggacaatga gggcgacgag gatgagggcc agggcgaggt
      301 agaagccgct gaagagggtg gcgtaccact gcgggaaggc cgcgaacatc gcgccgcccg
      361 ctgtgagcac ccagacctcg ttgccgtccc agaccgggcc gatggtgttg agcatgacgc
      421 gcttgcgggc ctcggtgtcg gcggcgccgg cggcgccctt ctggtggctg cggccgagga
      481 tgggcagcag catgccgacc ccgaagtcga agccttcgag ggcgagatag ccgatccaga
      541 ggacggcgat gaggacgaac cagacaatct ggagggttga caggtcagac atggtcaggg
      601 tccttggctc agtaggcgaa gacgaagggt tcgtcgtcgg cccgatcggc ggggtccttg
      661 ggtgcctcga cgggcaccgc ccccggctgg atgtagcgca gcaggagccg caactcgacg
      721 acggcaagcg cgccgtagag cagggtgtag atgatcatcg agatccacac gctggtggcc
      781 gagaccgagg tggagacgcc ggtctcggtc gtcatcaggc cgttgacgag ccagggctga
      841 cggccgacct cggtgaagat ccacccgaag gagttggcca gcaccggcag cagcggcgtc
      901 cagatggcga tgtgcttcca catgccgctg gtgacgacgc cttggccgcg ggtcttccac
      961 agcgccatgg cggcgaacaa catcgtgagg aacccggcgc ccatcatcag gcggaagttc
     1021 cagtaggtca tccagatcac cgggacgtag gacgccgagg cggtctgcgg gttgttgccg
     1081 aactcggcct tgtcagccgc ctggagatcg ttgatgcctt ggatctcgcc ggagaacgag
     1141 ccggtgccga ggaaggacag cagaccgggg acctcgagga tcggtcgggc cgccgtgccg
     1201 tcgagcgagc cgatcgtgag gatcgagaac ggcgcgccgg agcgggtctc gtacagtccc
     1261 tctgccgcag ccatcttcat cggctggact tcggtcatca ccttgccctg gacgtcgccg
     1321 gagaccagga cgccgatgct ggcgacgagg gcgacgatcg cgccgaagcg aacggccttg
     1381 cggtacatcg agatgtccgg gcccttgtcg agcgcctgct tgcgcaggtg gtagagggcg
     1441 atgcccatga cgaacgcgcc ggcggtcatg taggccccga gcacgacgtg cgggaacgtc
     1501 accagctgca ccttgttgaa cagcacggcg gcgaagtcgt cgagctcggc gcggttggtc
     1561 tgagcgttgt aggtgtagcc gaccgggttc tgcatgaacg agttggcggc gaggatgaag
     1621 aacgccgaga acaccgtgcc gatgtgcacg agccagatgg aggccaggtg gatcttcttg
     1681 ggcaaccggt cccaaccgaa gatccacagg ccgaggaagg tcgactcgag gaagaacgcc
     1741 agcagcgcct cgaaggccag cggcgcaccg aagatgtcac cgacgaaccg gctgtagagg
     1801 ctccagttca tgccgaactg gaactcctgc acgatcccgg tcaccaggcc gagggcgaag
     1861 ttgatcatga agagcttgcc gaagaacttg gtcaggcgca gccagtggtc gtcaccggtc
     1921 cggtgccaga aggtctgcat gatggcgatg agcgccgtga cgccgatcgt gatgggtacg
     1981 aagaggaagt ggtacaccgt cgtgatggcg aactgccacc gcgcaaggtc caatacatcc
     2041 attggaaggt ctcccgcatg agtgccaatg aaagggctta cccgccgacg ctaccgctgt
     2101 gcgtcggggt ggggatgcca cctcaggcct aggtgtgacg cagtagactc caatacccct
     2161 aggggtactc gggcgaaggg tgaactccgg cgccggggca ctgaccccct gattttgtcc
     2221 gtacctcagg taccgacctg ctactccggg tagccgccgg ggttggcgct ctgccaccgc
     2281 cacgtgtcgg cacacatgtc atcgatggac cgctccgcgc gccagcgcag ctcctcgtgg
     2341 gcgcggctcg ggtcggccca gtatgccggg aggtcgccgg cccgccgccc gacgatgtcg
     2401 taggggatcg gccgcccact ggccttctcg aacgccgcga tgacctgcag caccgatgtg
     2461 ccgatgcccc cgccgaggtt ccacgtgctg atcttgcgat cgtgcccggc gatccactgc
     2521 agcgcggcga cgtgtccggc ggccaggtcc tcgacgtgga tgtagtcgcg ctctgcggtg
     2581 ccgtcagccg tcgggtagtc gccaccaaac acctgcagcc gctcgcgccg gcccaccgcg
     2641 acctgcgcga caaacggcat gaggttgttg gggatgcctt gcgggtcctc gccgatcgtc
     2701 cccgacgggt gcgcgcccac cgggttgaag tagcgcagga tcgccacctt gagccgctca
     2761 tcgatcgccg ccacgtcacg caggacgtgc tcaatcatca ccttggtccg gccatagggc
     2821 gacgaggccg agagcggctc gtagtcctcc tggtagggga ccggcgccac ctcgccatag
     2881 accgtcgctg aggacgaaaa gacgagcagg tcgcacccgt gccggcgcat ggcttgcagc
     2941 agcgagaacg tcgactcgag gttgttgacg tagtagtcca gcgggatctg cacgctctcg
     3001 ccgaccgcct tgagcccggc gaagtggatg accgcgtcga acttctcggc cgcgaagagc
     3061 cgctcggtct tgtccttgtc ggtgaggtcg aacccatgca cctccaggtg ggtgccggtc
     3121 agcgactcca gccgcccgac gaccgtcggc ttcgcgttgg cgaacgagtc gacgatgacc
     3181 acgtcgtgcc ccgccgccgc caactgcacc accgtgtgcg acccgatgta tcccgcccca
     3241 ccactgacca agacgcgcat acgcacaccg tatgccgtgc ggcatacctc ccgcgcaccc
     3301 tcgcccccct tccgcccccc tgccgggtcg aggtattcca ccgtcgtctg gcgacggccg
     3361 aatacctcga ccgggcgcac gaaccatggc cgtgcgccga gaagcacatt tatacaaggc
     3421 atcttgtatg ccgtcgtgag cgccgacacg atggtcctca tgtcaaggaa gccggcggct
     3481 gaccgccacg cgacaacaga gccgcccgag tcgggatcgt cgttgattga cagggtgatt
     3541 gccttccacc atccggtgcg gcggcgattg ttcgatgcgc tgatcggtga tgggccttcg
     3601 ccagtggggg ttctggctcg gcggtgccac gttgcggtcg gctccgcgag ctaccacttg
     3661 ggtgtcctgc accggaacgg atgggtgcaa cccgctccgg agctgagtgg agacacgcgc
     3721 gaatcgtggt ggcgagcccg tcatgcggag ctggcctggt ccactcctga cctcacggtg
     3781 agtgcggcag gccgacacgt gctcggcctg gctgaacgcg aggatgtcac ctacgagttc
     3841 cgctccgtca tggcgtggct cgcgcatcgt gaggacctgc ccagcaagtg gcagcactcc
     3901 atgtcgtccg gggcgctcat caccgccacg gacacccagc tgattgacct caagctgcgg
     3961 atggaggcac tcgtcgcaga ctggtcggaa gcgtgtattc gcgatcagaa cgagactccg
     4021 gacaaagacc gttggcctgt gcgggtgatc gcacgtgtct tccctgcgac gctcggtgac
     4081 gtggcagggg cataggcccc aggtcctgtg atgaacgtgt ggagtttgta gcacgttgta
     4141 tgacaatgac gttctctgga cggtgacttg cgggggatca atgcctggcg ggcgaccgcg
     4201 gcagacctga agctgtacca agaaggagac cgcgatggcc aagaggattg acccggcact
     4261 ggccgaacga ctccggcacg agtctgaaca gacgaaggac gacgtctacc ctggcggcac
     4321 cacgggccgg aggccgaacc gacagaaggg gtactcggac tgtttgagcg ccgaggaaga
     4381 aagctgaggt tcaacgagtt gcgacagcca agcatctgcc ggcttcaact ctcgtccgat
     4441 cgtggatcct cgaacgactc gaccaggaac gctccgcctg atctgcgcat cgctgcgcca
     4501 attcgtcacg accaggcggc gcgcatgacc tcgacatcgg acagatcggc tatgcggtcg
     4561 gtgacggctt gaaggtcggc ggtcttgacc ggcccggcca ggcggcaggc ttcttggtgt
     4621 aggcgtcgac gcaggtgctc atcgagcgag atgcccagct gagcagcctg cgcatcgagc
     4681 cgagcgagat cagcggcagg gacattgggg atcaggacgt ctggcatgtg tgcctcctcg
     4741 ccaacacgtg ttgcggcatc tgaagagtaa gtctcctcgc ggacaacgat cgaggtgcca
     4801 gtgcctctac gcctcggata gaagcattac acggtggtat ctctcgtgag aggattctat
     4861 ccatgcagga tctggagatg gatctcctcg cgccgacctt caccaccaag gccgcgctgg
     4921 ccttgggggt tcacccgcgg gatctgtacg cctggcggga cggcggtctg ctgatcgagt
     4981 tgtctcgcgg tgtcttccgc aaggttgatg ccccgccggc gacatatccc gatctgctgg
     5041 cggtcaccat gcgagcgccg acgtccatcg tgtgctgcgt gtctgccgct gccgtccacg
     5101 agctgacgga cgagttgccc ccagctgtcc agctcgcggt gccgaacggt tctcatcccc
     5161 ctcacatcga ctacccgccc acgcaggtgt ttcgtttcgc gtcggcgacg ttcgcgttgg
     5221 gcctgtcgga ggtcgaggcc gcgcctggtg aatgggtgcg catctatgac gccgcccgca
     5281 cggttgttga cctgatgcga cttcgacacc ggttcgggga gacgttggcc cacacagcgt
     5341 tgaatcggtc gttgacatcg ggagcggctc gtccggcgga tgttctgcat tgcgcccagg
     5401 tgttgggtgt ggctgggccg gtccgcctgg cgctcgacat cgcgag
//
LOCUS       DAOMZY010000250         5405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_14247_length_5405_cov_5.436449, whole genome shotgun sequence.
ACCESSION   DAOMZY010000250 DAOMZY010000000
VERSION     DAOMZY010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14247_length_5405_cov_5.436449"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..533
                     /locus_tag="PLK64_12845"
     CDS             237..533
                     /locus_tag="PLK64_12845"
                     /inference="COORDINATES: protein motif:HMM:NF016875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPV80683.1"
                     /translation="MRVEYHDEVLRRLAEEREYAPKGWDPTVIRSYRRKVQLIVAAVD
                     ERDLYEMRGLRMEKLKGDRLGQTSMRLNDQFRLILKFKTEGERVAVLLEVVDYH"
     gene            554..1651
                     /locus_tag="PLK64_12850"
     CDS             554..1651
                     /locus_tag="PLK64_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015936296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HPV80684.1"
                     /translation="MNAALAEVFPAGEFLADELEARGWTQAEFAAILGRPAQFVSEII
                     SGKKEITRESAAQIGAALGTSAEMWLSLQDQYLLWLQKQDARSQSDLDDVRRRARLNA
                     KGPIPILRKAGVLTGKSLDELEGEVMRFFELKSLDDEPRLAAAAKRSNHGEAPSALQT
                     SWLYMVRHAARQKAVTSTYSAEGLQSVGATLPRILKNPGDFSGLPDMLGEVGVRLVYV
                     PALPRAKIDGCAMIVDETPVIGLSGRGKRLDKVLFALVHEIAHLTNGHVKDDAPIIET
                     IDVDDDPNDHSHEALQEREANETAARWVLPDGLPRLPDQLSGPWISQAAQDADLAPIV
                     LVGHLQHLRKLDWRTTLAKGAPNVDEVIARW"
     gene            1749..3611
                     /locus_tag="PLK64_12855"
     CDS             1749..3611
                     /locus_tag="PLK64_12855"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing HNH endonuclease family
                     protein"
                     /protein_id="HPV80685.1"
                     /translation="MKADALNPRELFGTTVHYEIPAFQRPYVWTLDDQWQPLWQDVRR
                     VAEKIIAADGDEASLEAVGGHFLGAVVFKLKSATAGDVTRQSVIDGQQRSTTLQIMLD
                     AVHEAVLSRGHEVEAEAIEELILNQAKRFAGRPERFKLWPSRSDRAAFEFAMDGAGAH
                     NGDHLIVEAHEFFRTEVGNWLAGIVDEGEQPLGDEKARVAALSDVVQSRLYVVAINLT
                     GHDDDQVIFETLNDRGTPLLKADLIKNWIFQVGEQLHANVDSWPEKYWADFDDTWWRD
                     EITQGRHMRSRIDIFLQYWLTMRRRDDVLTDEVFREFVTYAKPLMTTTTDAEALLSEL
                     RRDANTFRGFAQLSRDSVEGQFYGRVVESLELASTTPLLLWMLSENHSVPQGQVAIAL
                     GALESWVIRRTLLRSTMKDVNKMMVAILGTLDDVLVDEVGGAVRDYLANQTSDARTWP
                     SDDDLIATLPGIKLYGNVRQSRLRVVLEALEMNMRTQRHEAISLPSKLSVEHVMPQAW
                     RSHWDPEPKLSPEAAADRDKLVNTLGNLTLVTSDLNGTLSHRPWTDAATAPLKTTGEN
                     KGLGKRSLLNKYSVLLLSREIVDHHAEAWTEEDIRKRSVKLAKVLCDVWPRPVS"
     gene            3958..4869
                     /locus_tag="PLK64_12860"
     CDS             3958..4869
                     /locus_tag="PLK64_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80686.1"
                     /translation="MGARDLLAAQKRQVAAKEQHEARVAASEERRTAANQQLIHYSGL
                     QHECFAGVVRRMIVWLRAHAKVVRESDRLLVDGLEASLALMPATPGHEAVIAGWVTGA
                     SSTAIAGLGTAGAISSAVEKYGVAGTGAKITDLTGAAKEKAAAAFRGGGPIKDGGGGI
                     ALGNLATRAAVGGVTLLAAGSTCKVQGTKALTRAEEFVTHVQVALAELDLQDAKLRAV
                     VQRTDELTDVLTRLDLRAKAAMDRLESLRFDPDEHMDALGKAVELVTAVQAVAQTPLV
                     NECGDLSEESETLKIRYREMSGDNSDG"
     gene            4874..5287
                     /locus_tag="PLK64_12865"
     CDS             4874..5287
                     /locus_tag="PLK64_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80687.1"
                     /translation="MSIEGKVLDASAIVAAPEALGALTRLVDTAGKYVAIHEEEARKR
                     EELRTYRETEVATIKAAEGTLQRYFANIFSERREVYQHLFRAMDHALESGDTESLHSV
                     VTGIVDLAKDSPLAAAGDLSKLRAAFDDPNQVWSF"
BASE COUNT         1085 a         1697 c         1700 g          923 t
ORIGIN      
        1 gtcttctcgt gaggctggac gccggtgtcg ttgggcatgt tggctcctgt ggtcgatgcg
       61 gtagtgcgag cacttgcccg ctgatgctga acttagcata ctgttcttca cccaagaggt
      121 gatggttaga tgaccacaca gcgtgtcgca cgatgtcagg ccgaagtgga agcattggcc
      181 gcctaggatc cgtcatgtgc gggtcttcat ctctggggtg atggaatgag gtcacgttgc
      241 gcgtcgagta ccacgacgaa gtgctgcgcc ggctcgccga ggagcgcgag tacgcaccca
      301 aaggttggga tccgacggtc attaggtcgt atcgaaggaa ggtgcagctc atcgtcgcgg
      361 cagtggatga gcgcgacctc tacgagatgc ggggtctgcg gatggagaag ctgaaaggcg
      421 atcgactagg gcagacgtcg atgcgtctca acgatcagtt ccggctgatc ctgaagttca
      481 agaccgaagg agaacgggtg gccgttctcc ttgaagtggt cgactaccac tgaacatcga
      541 tggagagatc gagatgaacg ctgcactagc cgaggtgttc cccgcagggg agttcctcgc
      601 tgacgaactt gaggctcgtg gctggactca ggccgagttc gccgccatcc tcggtcgacc
      661 cgcgcagttc gtctccgaga tcatctctgg caagaaggag atcacccggg agtccgcagc
      721 gcagattggt gcagccctgg ggaccagcgc cgagatgtgg ctcagcctgc aggaccagta
      781 cctgctttgg cttcagaagc aagatgcgag gtcccagagc gacctggacg acgtacgtcg
      841 tcgtgcccgc ctcaacgcca agggcccaat ccccatcctg aggaaagcag gagttctgac
      901 aggcaaatcg ctggacgagc tcgagggcga ggtcatgcgg ttcttcgagt tgaagtcgct
      961 cgatgacgag ccacgactag cggccgccgc caagcgctcc aaccacggcg aagcaccgtc
     1021 cgcgctccag acctcatggc tgtacatggt ccgccacgct gcccgccaga aggcggtgac
     1081 gtccacgtac tcggcggagg gcctccaatc cgttggcgcg acgcttccgc ggatcctcaa
     1141 gaatcccggt gacttctccg ggctgcccga catgctgggc gaggtaggtg tccgactcgt
     1201 ctacgtcccc gcactcccaa gggcaaagat cgatggctgc gcgatgatcg tcgacgagac
     1261 gcccgtcatc ggcctgtctg ggcgagggaa gcgactggac aaagtgctct tcgccctggt
     1321 ccacgagatc gcccacctga ccaatggtca cgtgaaggac gacgcaccga tcatcgagac
     1381 aattgacgtc gacgacgacc ccaatgacca ctcacatgaa gcgctccagg aacgcgaagc
     1441 gaacgagacc gccgctcggt gggtcctccc tgacggtcta ccgcggctac ccgaccaact
     1501 cagcggaccg tggatcagcc aagccgcaca agatgcggac ctcgcgccaa tcgtcctcgt
     1561 gggacacctg caacatctca ggaaattgga ctggcgtacc acgttggcga agggcgcgcc
     1621 gaacgtcgat gaggtcattg ccagatggtg atcgcccgcc cccgcgagca cgcacaccct
     1681 gcgttgcagg gcacgagctc atggcgtacg cgccctccta catccggaac gaactttgag
     1741 gggaccacgt gaaggccgac gcactcaacc cgcgtgaact ctttggcacg accgttcact
     1801 atgagatccc ggcattccag cgcccatacg tttggaccct cgacgaccaa tggcagcctc
     1861 tttggcagga cgtgcgccgt gtggccgaga agatcatcgc tgccgacggg gacgaagcgt
     1921 cgctcgaggc cgtcggcggc cacttcctcg gcgcagtcgt cttcaagctg aaatcagcga
     1981 ccgcaggcga cgtcacaagg cagtctgtca tcgacggtca gcaacgcagc acgacactgc
     2041 agatcatgct cgacgcagtc catgaagcag tgctgagccg tggccacgaa gtggaggcag
     2101 aggcgatcga agaactcata ctcaaccagg caaagaggtt cgccggccga cccgaacgct
     2161 tcaagctatg gccatcacgc tcggaccgag ccgctttcga gttcgcgatg gacggggctg
     2221 gcgcgcacaa cggtgatcac ctgatcgtcg aagcccacga gttcttccgg actgaggtcg
     2281 gtaactggct ggccggcata gtcgacgagg gcgagcagcc tctcggggac gagaaggccc
     2341 gcgtcgccgc gctgtctgac gtggtccagt cccggctcta cgtcgtcgcc atcaacctca
     2401 ccggccacga cgacgaccag gtcatcttcg agaccctcaa cgaccgcggc accccgctgc
     2461 tcaaggccga cctcatcaag aactggatct tccaggtggg ggaacaactc cacgccaacg
     2521 tcgactcctg gcccgagaag tactgggccg acttcgacga cacctggtgg cgtgacgaga
     2581 tcacacaggg ccgccacatg cgctcccgga tcgacatctt cctccagtac tggctgacca
     2641 tgcgccgccg tgacgacgtc ctcaccgacg aggtgttccg ggagttcgtc acctacgcca
     2701 agcccttgat gaccacgact accgacgccg aggcactcct cagcgaactg cgccgagacg
     2761 ccaacacgtt ccgcgggttc gcccaactgt cgcgagacag tgtcgaaggc cagttctacg
     2821 ggcgagtcgt ggagtcgctc gagctagcct cgacaacccc tctgcttctg tggatgttgt
     2881 cggagaacca ctccgtcccg cagggccagg tcgcgatcgc cctcggcgcg ctcgagagct
     2941 gggttattcg acgcacgctt ctgcgtagca cgatgaaaga cgtcaacaag atgatggtcg
     3001 ccatcctggg gaccctcgat gatgtcctcg tcgacgaggt tggcggggcc gtcagggact
     3061 acctcgccaa ccagacctcg gacgcgcgaa cgtggccaag cgatgacgac ctgatcgcta
     3121 ccttgcccgg gatcaagctc tacggcaacg tgcggcaatc ccgactgcga gtagtcctcg
     3181 aggcgctgga aatgaacatg cgtacccagc gccacgaggc tatctctctg ccttccaaac
     3241 tctcggttga gcacgtgatg ccgcaggcat ggcgcagcca ttgggacccc gagcccaaac
     3301 tgtcgcccga agcagcagcg gaccgggaca aactcgtcaa caccctgggc aacctcaccc
     3361 ttgtcacctc cgacctcaat gggacgttgt cgcaccgacc ttggactgac gcagccaccg
     3421 ccccgctcaa gacaactggc gagaacaagg gcctgggaaa gcggtccctg ctgaacaagt
     3481 acagcgtgct gctgctcagt cgtgagatcg tcgaccacca cgccgaagcc tggaccgagg
     3541 aagacatccg gaagcgatcg gtgaaactgg ccaaggtcct gtgcgacgtt tggccacggc
     3601 cggtttcgtg accacccaca cgccacccga tgaactggcg aactcgcttg tctgggtctg
     3661 ctcctcgagt ccgttgctgc tgacccgacg aactaaggtc accgtgggtc gtgagcgggc
     3721 ctaagtggtc gtctatgtgg aaatggtggg agagtgccgc ccgcaacccc tgacctctgg
     3781 aacccacgga gtgaacttcg cgcaggtggt gggcgtcccg atccgccgcg tcgcggtgct
     3841 caccgagtgc cacaatcgcg tatgcctacc cttcctgccg gcgtctttcg ccccgtgccc
     3901 gctgccctgt ttggcgttgg tgctgtagcc ggcgtggtgg gctcaggact cggtgccatg
     3961 ggtgcacgtg acctccttgc tgctcagaag cgtcaagtcg cggcaaagga acaacacgag
     4021 gcccgcgtag cagcctcgga ggagcggcga actgcagcga accagcagct gatccactac
     4081 tcggggctgc aacacgagtg tttcgccggg gttgtacggc gcatgatcgt gtggctgcgc
     4141 gcacacgcca aagtggtacg tgagagcgac aggctcctcg tggacggact cgaagcctcc
     4201 ctggccctga tgccggccac tcccggccac gaagccgtca tcgccggctg ggtgaccgga
     4261 gcgtccagca cggcaatcgc aggcctgggc acagcaggtg ccattagcag cgctgtcgaa
     4321 aagtacggcg ttgccggcac tggagccaag atcactgact tgaccggggc cgcgaaggag
     4381 aaggctgctg ccgcctttcg cgggggcgga ccgatcaagg acggcggcgg cggtatagcc
     4441 ctcggcaatc tggccacccg agctgcagtg ggcggtgtga ccctgctcgc agccggctcc
     4501 acttgcaagg tccaaggcac aaaggccctc acccgcgccg aagagttcgt gacccacgtg
     4561 caagttgcgc ttgccgaact ggacctccag gacgcgaagc tgcgcgcagt ggtccagcgc
     4621 actgacgaac tcacggacgt gctgacgagg ctggacctgc gagcgaaagc cgccatggac
     4681 aggcttgaga gtctcagatt cgacccggat gagcacatgg atgcacttgg gaaggcggtt
     4741 gagctggtaa cggctgtgca ggcagtcgcg cagactcctt tggtcaatga gtgcggtgac
     4801 ttgtcagagg agtctgagac cctgaagatc cggtaccgag agatgagcgg ggacaacagc
     4861 gatggctgat ggaatgtcga tcgagggaaa ggtcttggac gccagcgcga tcgtcgccgc
     4921 gcccgaggcg ttaggagcgc tgacgcgact ggtcgacact gcgggcaagt acgtggcgat
     4981 tcacgaggaa gaggcacgca agcgggagga gctgcgcacc taccgggaaa cggaggtcgc
     5041 caccatcaag gccgccgaag gcacgcttca gcggtacttc gccaacattt tcagcgagcg
     5101 acgagaggtc tatcagcacc tcttccgcgc gatggaccac gccctggaga gtggagacac
     5161 cgagtcgctc cactcggtcg tgacgggcat cgtggacttg gccaaggact cccctctggc
     5221 ggcggctggg gatctatcga aactacgagc cgccttcgac gacccgaatc aggtctggtc
     5281 cttctgacgc cccctcgggg gaggatgacc aaccgggttc tcaaggttgt acggggtccg
     5341 cattggactg ccgttcctgc aacgttcaat gcgcgtcgct atgcagtttt ggatgcatct
     5401 cgaca
//
LOCUS       DAOMZY010000251         5399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_14261_length_5399_cov_5.934319, whole genome shotgun sequence.
ACCESSION   DAOMZY010000251 DAOMZY010000000
VERSION     DAOMZY010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14261_length_5399_cov_5.934319"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..1016)
                     /gene="prcA"
                     /locus_tag="PLK64_12870"
     CDS             complement(198..1016)
                     /gene="prcA"
                     /locus_tag="PLK64_12870"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HPV80688.1"
                     /translation="MSMPFYVSPEQLMKDKADYARKGIARGRSVVVLQYDRGIVFVAE
                     NPQSLRKISEIYDRIAFAAVGMYYEFENLRVAGIRYADLRGYSYDRSDVSARGLANAY
                     AQTLGTVFTQEPKPYEIEICVAEVGATPERDQIFRLTYDGSVADQHGFVVMGGSNQAA
                     EERLKAGWQEGMPLADALILAVAALAADPAGGEPRTLSPDQLEVAVLDRNRPRRTFRR
                     IVGPLLADLLNVDSPTSHAPEFDEDVPGHHDTLSGQPAAHPDHDDDGNQGEDSR"
     gene            complement(1045..1887)
                     /gene="prcB"
                     /locus_tag="PLK64_12875"
     CDS             complement(1045..1887)
                     /gene="prcB"
                     /locus_tag="PLK64_12875"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HPV80689.1"
                     /translation="MNRSTPPPDGRAGSLPAAYLRPGSASFTEFVAGVDPELLPGRRP
                     LPAGMSLEAPHGTTIVAAEFAGGVVVAGDRRATMGNLIANRDMEKVYAADEYSVVGIA
                     GTAGIAVELVKLYQVELEHYEKIEGTIMSLEGKANRLASMIRGNLGLAMQGLTVVPMF
                     AGFDLDERRGRIFSYDVTGGCYPERDHHSIGSGSLFARGSLKKVWRPGLSEADTVRAC
                     LEALYDAADDDSATGGPDLGRRIWPTLAVLDETGVRFLSDDDLAVRVEAIVADRFGNP
                     GGAR"
     gene            complement(1884..2081)
                     /locus_tag="PLK64_12880"
     CDS             complement(1884..2081)
                     /locus_tag="PLK64_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043806102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HPV80690.1"
                     /translation="MASQEQSRPQRREGDPDAPPPEVPVGGAQTQVSNADIDSVLDEI
                     DEVLEVNAEEFVRSFVQKGGQ"
     gene            complement(2138..3667)
                     /gene="dop"
                     /locus_tag="PLK64_12885"
     CDS             complement(2138..3667)
                     /gene="dop"
                     /locus_tag="PLK64_12885"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HPV80691.1"
                     /translation="MGVQRVMGVETEYGVAAPGDPTANPMWLSGQVVTAYARAKGLLT
                     GRGAWDYADEAPLRDARGWELGRAWADPSQLTDIDDPTMANVVLTNGARFYVDHAHPE
                     YSGPEVTTPRAVVAWDRAGDLVMQEAAWLLNPDPARPTVALYKNNTDGKGASYGTHEN
                     YLMRRETPFHDIIRHLTPFFVARQVIAGAGRVGIGQDSRVAGYQLTQRADFFETEVGL
                     ETTLKRPIINTRDEPHASADLYRRLHVIIGDANQCDVANLLKVGTTSLVLALIEDRAM
                     PGDLTVLNPVSTLHAISHDPTLRTLITLRDGRKLTALELLWIYHDACAAYLQERAGDP
                     LGGDADTREVMTLWADILTRLGTDPMSCARELDWVAKLRLLQAYRDRDGLAWTEPRLA
                     ALDIQWSDIRPNKSVFAKLRAAGQVTELVGAAEAQAAVTTPPEDTRAWFRGQCLSRYA
                     ANVVSASWDSVIFEVPGQTSLQRIPMLEPLRGTRAQVGEVLERSPDVATLLRELIRSG
                     D"
     gene            3776..4762
                     /locus_tag="PLK64_12890"
     CDS             3776..4762
                     /locus_tag="PLK64_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPV80692.1"
                     /translation="MPLRTAIFTVVLDRFGRSSILDLDESEIATARQWAAPARWPHTW
                     VTGGVSGRVSIREAEFTARDGVERALRIYAPLYSPGPLPIVAFFHGGGYVLGNTRMYD
                     PLCAFLAKSVPAVVVSVDYRQAPEHRAPKAVEDSIDGLRWAYENAAAFRGDPARIAVC
                     GDSAGGGLAALVCHAAYDEGGPAIAHQSLLYPSTDATQSFPSAAEHGGAPVLSAEKIR
                     VFTDHYLGDSVDPADPGVSAYWRKDLHGMPPALIQTADLDPLRDEGLAYAERLADAGV
                     PVRATNYLRTVHGFMSFPGATTIGEQARYELLSELRRHLNPTRLAHLVPVDV"
     gene            4821..>5399
                     /locus_tag="PLK64_12895"
     CDS             4821..>5399
                     /locus_tag="PLK64_12895"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HPV80693.1"
                     /translation="MPRMPLRTRLLTFVLDHGTHPKITDLDVPGIQHARTRVAPLRRP
                     FTWVTGGMPPDVQTRDLWFPVRDGSQRPARAYLPSTPGPHPVIVFFHGGGWVLGSTRQ
                     YDPLCGLLAARVDALVLSIDYRMGPEHHAPQAVLDCVDATRWAATAAADLGGDPARMA
                     VCGDSAGGNLAALVCHAAYDSGGPAIAHQALIY"
BASE COUNT          773 a         1912 c         1853 g          861 t
ORIGIN      
        1 agtgacagtc ttcgtcgcag cacgaatctc agcccggcga gatgccgagt cacgaatcac
       61 ttcgctgctg tccataaccc cgacgctaac agcgaccacc gacagacccc ccaccccaaa
      121 aggcctctcc cacaaccgaa cacatcacaa acaggtaacg ttcgggcggt ctgagaagca
      181 cacctcccga ggtcgaatca gcggctgtcc tcgccctggt tgccgtcgtc gtcgtggtcg
      241 gggtgggccg cgggctggcc ggacagggtg tcgtggtggc cgggcacatc ctcgtcaaac
      301 tcgggcgcat gcgaggtcgg gctgtcgacg ttgagcaggt cggccaacag cggaccgacg
      361 atccgccgga aggtccgccg gggccggttg cggtccagga cggccacttc gagctgatcg
      421 gggctgaggg ttcgaggctc tcccccggcg gggtcggccg cgagggccgc gacggcgagg
      481 atgagggcgt cggcgagcgg cataccctcc tgccaaccgg ctttgaggcg ctcttccgct
      541 gcctggtttg acccgcccat gacgacgaac ccgtgctggt cggcgaccga cccgtcgtag
      601 gtgagtcgga agatctggtc gcgctcgggg gtcgcaccca cctcggcgac gcagatctcg
      661 atctcgtagg gcttgggctc ctgggtgaaa accgtcccca gggtctgggc gtacgcattg
      721 gccagccctc gcgccgacac gtcggagcgg tcgtaggagt agccgcgcag atcggcatac
      781 cgaatgccgg caacccgcag gttctcgaac tcgtagtaca tgccgacggc ggcgaacgcg
      841 atgcggtcgt agatctcgga gatcttgcgc aggctctgcg ggttctccgc gacgaagacg
      901 atgccccggt cgtactgcag cacgacgacc gagcggccgc gggcgatgcc cttgcgcgcg
      961 tagtcggcct tgtccttcat cagttgctcg ggtgagacgt agaacggcat gctcatcgcg
     1021 cacctcctgc tcgctgggcg gcgctcatcg cgcgcccccc gggttgccga agcggtcggc
     1081 gacgatggcc tcgacccgga cggccaggtc gtcgtcactg aggaagcgca ctccggtctc
     1141 gtccagcacc gccagggtcg gccagatccg ccgcccgagg tccggtcccc cggtcgccga
     1201 gtcgtcgtcg gcggcgtcgt agagcgcctc caggcaggcc cgcacggtgt cggcctcgga
     1261 cagccccggt cgccagacct tcttgagcga tccgcgggcg aagagcgacc cggagccgat
     1321 gctgtggtgg tcgcgctcgg ggtagcagcc gccggtcacg tcgtaggaga agatccgccc
     1381 gcgccgctcg tcgaggtcga acccggcgaa catcggcacg acggtgagcc cctgcatggc
     1441 caacccgagg ttgccgcgga tcatcgaggc cagccggttg gccttgcctt cgagggacat
     1501 aatcgtgccc tcgatcttct cgtaatgctc caactcgacc tgatagagct tgaccagctc
     1561 gacggcgatc ccggccgtcc cggcgatgcc gacaacggag tactcgtccg cggcatacac
     1621 cttctccatg tcgcggttgg cgatgaggtt gcccatcgtc gcgcgccggt cccccgcgac
     1681 gaccactccg ccggcgaact cggccgcgac gatggtcgtg ccgtgcggcg cctccaggga
     1741 catcccggcg ggcaggggac gccgaccagg caacaactca gggtccacgc ccgcgacaaa
     1801 ctcggtgaac gacgcgctcc ccggcctcag gtatgccgct gggagacttc cagcgcggcc
     1861 gtccggcgga ggggtgctgc ggttcactgg ccgcccttct ggacgaagct gcggacgaac
     1921 tcctcggcgt tgacctcaag cacctcgtcg atctcgtcga ggacgctgtc gatgtcggcg
     1981 ttggacacct gggtctgcgc gccgccgacg ggaacctcgg gaggcggcgc gtcggggtca
     2041 ccctcgcgcc gctgcggacg gctctgctcc tggctggcca tgggtcctcc acctgctcgt
     2101 gcgtcgatgt cggcctcggg gaccgacact gccaaaccta gtcgccggag cggatgagtt
     2161 cacgcagcag cgtggcaacg tcggggctgc gttcgagcac ctcaccgacc tgggcgcgcg
     2221 tgccgcgcaa gggctccagc atggggatgc gttgcaacga cgtctgacca ggcacctcga
     2281 agatcaccga gtcccacgac gcggagacga cgttcgcggc ataccggctc aggcactggc
     2341 cgcggaacca cgcccgggtg tcctccggcg gggtggtgac cgccgcctgc gcctcggccg
     2401 cgccgaccaa ctcggtgacc tggccggcgg cgcggagttt ggcgaagacg ctcttgttgg
     2461 gccggatgtc actccactgg atgtcgaggg cggccagccg cggctcggtc cacgcgagcc
     2521 cgtcccggtc gcgataggcc tgcagcagac ggagtttggc gacccagtcc aactcgcggg
     2581 cgcagctcat cgggtcggtg cccaggcggg tgaggatgtc ggcccacagg gtcatcacct
     2641 cgcgcgtgtc agcgtcaccg cccagggggt cgcccgcacg ctcctgcagg tatgccgcgc
     2701 acgcgtcgtg gtagatccac aacagttcca gcgcggtgag cttgcggccg tcgcgcagcg
     2761 tgatgagggt gcgcagcgtc ggatcgtggg agatggcgtg cagggtcgac accgggttga
     2821 ggacggtgag gtccccgggc atggcgcggt cttcgatgag cgccaacacg aggctggtcg
     2881 tgccgacctt gagcaggttg gccacgtcgc attggttggc gtcgccgatg atgacgtgca
     2941 gccggcgata gaggtcggcg ctcgcgtgcg gctcgtcgcg tgtgttgatg atcggccgct
     3001 tgagcgtggt ctccaggccg acctcggtct cgaagaagtc ggcccgctga gtgagctgat
     3061 agcccgcgac ccgcgagtcc tgcccgatgc cgacccggcc ggcgcccgcg atgacctgcc
     3121 gcgccacgaa gaacggtgtg aggtgacgga tgatgtcgtg gaagggcgtc tcgcggcgca
     3181 tgaggtagtt ctcgtgggtg ccataggaag cgcccttgcc gtcggtgttg ttcttgtaga
     3241 gcgccacggt cggccgcgcc gggtcggggt tgagcagcca cgccgcctcc tgcatgacca
     3301 ggtcaccggc ccggtcccag gcgacaacgg cgcggggcgt ggtgacctcc gggccggaat
     3361 actccgggtg ggcgtggtcg acgtagaacc gcgccccgtt ggtgagcacg acgttggcca
     3421 tcgtcgggtc gtcgatgtcg gtcaactggc tcgggtcggc ccacgcgcgg cccaactccc
     3481 agccgcgcgc gtcgcgcagg ggggcctcgt cggcgtagtc ccacgcgcct cgccccgtga
     3541 gcagcccctt ggcccgcgca taggccgtga cgacctggcc ggagagccac atggggttgg
     3601 cggtggggtc accgggcgcc gcgacgccgt actcggtctc gacccccatg acccgctgga
     3661 cacccatgtc cccaccctat ggcggctgtc acctcctgcg atccgggccc cggtgtggtg
     3721 acaatggagg gtgcctcgtc ccaccgctcg tctcggccgc gtccctcgtc cccgtatgcc
     3781 gctgcgcacg gcgatcttca ccgtcgtcct cgaccggttc ggccgatcgt cgatcctcga
     3841 cctggacgag agcgagatcg cgaccgctcg gcaatgggcg gcgccggccc gctggccgca
     3901 cacctgggtg accggcgggg tgtctggtcg ggtgagtatc cgagaggctg agttcaccgc
     3961 ccgtgacggg gtcgagcggg cgctgcggat ctacgccccc ctctactccc ccgggccgct
     4021 gccgatcgtc gcgttcttcc acggcggcgg ttatgtcctg ggcaacaccc ggatgtacga
     4081 cccgttgtgt gccttcctcg ccaagagcgt tccggcggtc gtcgtgtcgg tcgactatcg
     4141 ccaggccccg gagcaccgcg cgccgaaggc cgtcgaggac agcatcgacg ggctgcgctg
     4201 ggcctacgag aacgccgcgg cgttccgcgg cgatcccgct cggatcgcgg tctgcggcga
     4261 cagcgcaggc ggcgggctag ccgcgctggt gtgccacgcg gcatatgacg agggtggtcc
     4321 ggcgatcgcg caccagtcgc tgctctaccc gtcgaccgac gcgacccagt ccttcccgtc
     4381 tgccgccgag cacggcggcg cgcccgtcct gagcgccgag aagatccgcg tgttcaccga
     4441 ccactacctc ggcgactccg tggaccccgc cgacccgggc gtctcggcct actggcgcaa
     4501 ggacctgcac ggtatgccgc ccgcgctcat ccagaccgcc gacctcgacc ccctgcgcga
     4561 cgagggcctg gcgtatgccg agcggctcgc cgatgccggc gtcccagtgc gggccaccaa
     4621 ctatttgcgc accgtccacg gtttcatgag cttcccgggg gcgacgacga tcggcgagca
     4681 ggcacggtac gagctgctca gcgagcttcg ccgccacctc aaccccactc ggcttgccca
     4741 cctcgtcccg gtcgacgtct gacctccctc acgaagttga ccgctgcgcc cggggaggcg
     4801 gctttgacag actgacccgc atgccccgga tgccattgcg gacccgtctg ctcaccttcg
     4861 tcctcgatca cggcactcac cccaagatca ccgacttgga cgttcccggc atccagcacg
     4921 cgcgcacgcg ggtcgctccg ctgcgccgtc cgttcacgtg ggtcacgggg ggtatgccgc
     4981 ccgacgtgca gacgcgcgat ctgtggttcc ccgtccgcga cgggtcacaa cgcccggccc
     5041 gggcctacct cccgtcgacc cccggcccgc atccggtcat cgtcttcttc cacggcggcg
     5101 gctgggtcct cggaagtaca cggcagtacg acccgctctg cggacttctc gccgcgcggg
     5161 tcgatgccct cgtcctgagc atcgactacc ggatgggtcc ggagcaccac gctccccagg
     5221 ctgtcctcga ctgtgtcgat gccactcgct gggccgccac ggcggcggcc gacctcggcg
     5281 gcgacccggc caggatggct gtgtgcgggg actccgcggg agggaacctg gccgcgctcg
     5341 tctgccacgc ggcatacgac tcgggtgggc cggcgatcgc gcaccaagcc ctcatctac
//
LOCUS       DAOMZY010000252         5371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_14356_length_5371_cov_5.096125, whole genome shotgun sequence.
ACCESSION   DAOMZY010000252 DAOMZY010000000
VERSION     DAOMZY010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14356_length_5371_cov_5.096125"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1355)
                     /locus_tag="PLK64_12900"
     CDS             complement(<1..>1355)
                     /locus_tag="PLK64_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPV80694.1"
                     /translation="GRLGWLLTHLGELPGSGIVYTLTVAAAEDLATTLAQAGHRVAAY
                     TGRTDPAERERLEHALRDNKVKALVATSALGMGFDKPDLGFVVHVGAPSSPVAYYQQI
                     GRAGRATERADVLLMPGAEDRDIWAYFASVSMPTEERARAVIEALTAAGRPLSTAALE
                     AIVDIRRTRLELLLKVLDVDGAVRRVSGGWLATGESWTYDAERYERVAAARRAEQQLM
                     LDYERTGECRMAFLQRCLDDPTAADCGRCDTCTAPWYAADVADTALDAARAQLTRVGV
                     PIEPRAQWPTGMDRLGVSVRGRIAANEAMAPGRALARLSDLGWGQRLREVLREDAEVP
                     DAVVRAVIRVLSEWDWDERPAGVVGMPSRRHSLLVGSLAAAIAQIGRLPLLGTLDLLD
                     GGPIGEPGGNSAFRLAGVWERLGVSPDLASRVDGIPGPVLLVDDLVSSRWSLTVAARA
                     L"
     assembly_gap    1356..1455
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1456..2068)
                     /locus_tag="PLK64_12905"
     CDS             complement(<1456..2068)
                     /locus_tag="PLK64_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPV80695.1"
                     /translation="MDDDLEGNARAALRRLVGRADVDFHEGQLESALALVRDRERVLV
                     VQRTGWGKSAVYFVATALLRANGAGPTIIVSPLLSLMRDQIAAATRAGIRAVTMNSAN
                     TLDWAQVRQALADDAVDVLLVSPERLNNPAFRAEQLPDLAARAGLLVVDEAHCVSDWG
                     HDFRPDYRRIGDLLRALPPGTPVLATTATANARVVADVERELGG"
     gene            complement(2151..2543)
                     /gene="rnpB"
                     /locus_tag="PLK64_12910"
     ncRNA           complement(2151..2543)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLK64_12910"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2604..2981
                     /locus_tag="PLK64_12915"
     CDS             2604..2981
                     /locus_tag="PLK64_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPV80696.1"
                     /translation="MIDHFGINCSDMPTAARFYDGVLGVLGHRRVMDFGAAIGYGTEA
                     PVFWISTFDGVGPNREVHVAFTAPDAAAVREFHAKALELGAEELHAPRLWPEYHPGYF
                     GAFVRDPDGNNIEAVCHAAGPTD"
     gene            complement(3008..4561)
                     /locus_tag="PLK64_12920"
     CDS             complement(3008..4561)
                     /locus_tag="PLK64_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HPV80697.1"
                     /translation="MSEQPIHSPDPVSAALALCPDEALAPLSGADQEAAALAAIVLIE
                     QLKARLDALAVSAWRGLYAGYAREAAERLVPLVEAARAGAAVRGSLPGPAALRAQAAA
                     AVRGGVVAEVMAATGLPEGECWRRLRLATAEPARSEGLIAMLAAGEADLARVARLCDE
                     ISELDAEEAGEVALRVVAPCRDGSVASHALVGRRLRRALAQHRADDPAAAAKHAERAK
                     RARRTSAELFDDGTGVVRIVGEAARVCSVMSRIDALARGLRAGGGYSEVTLDQLRSDV
                     AFELLMRGDGRVVSDRPESGPTPVGDGASIGAASLAPAAVVGTAPLIPGQRFGAAPPA
                     HVDVIVTLATLLGCDDEPGELPGFGFVSASHVRQIAHAAGSVWRRLVVDDRTGALIEV
                     STHRYRPTEAMRTHVAARDRVCRGPGCQVSPIGADLDHDVPWPAGESSTANLSVKHRR
                     HHQLKTFGLWSTRQDPETASVTWTTLAGRSYVTEPHDYLDGRHAGAWAAHAVSTRHTD
                     DPADDPPPF"
     gene            4718..>5371
                     /locus_tag="PLK64_12925"
     CDS             4718..>5371
                     /locus_tag="PLK64_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HPV80698.1"
                     /translation="MPPFRKSRADAPVGYFAWEAAGLRWLGAATDAGGVPVATVLQVG
                     PTHLDLVRLDPVPPTRAAAAAFGADLARTHDPGGADVGGADVGGANPAFGAGPPHWLG
                     DGFFGPTDQPLPLSLTPHFRWSAFYAAERLTPITQGCRDAGVFDATEAALLDRLAATI
                     DRHDTDEAPSRIHGDLWSGNVIWTSAGATLIDPAAHHGHREADLAMLALFGSPHLDVT
                     "
BASE COUNT          805 a         1966 c         1753 g          747 t
ORIGIN      
        1 gcagggctcg cgcggcgacg gtgagggacc agcgcgagct caccaggtcg tcgacgagca
       61 gcaccggccc cggtatgccg tccacccggc tcgcgaggtc gggtgacact cccagccgct
      121 cccagacccc ggcaaggcgg aaggcactgt tgccgccggg ctcgccgatg ggaccgccgt
      181 cgagcagatc gagcgtgccc agcaacggca agcgaccgat ctgcgcgatg gctgccgcga
      241 gcgagccgac gagcagggag tggcggcgcg acggcatacc aacgacgccg gccggtcgct
      301 cgtcccagtc ccattcggac agcactcgga tgacggcccg gaccaccgca tcggggacct
      361 cggcgtcctc gcggagcacc tcacgaagcc gttggcccca cccgagatcg ctcaggcgcg
      421 cgagcgcccg gccgggtgcc atcgcctcgt tggcggcgat ccggcccctg actgagacac
      481 ccaatcggtc catccccgta ggccattggg ctcgcggctc aatggggacg ccgacccggg
      541 tcagttgggc gcgagcggca tcgagggcgg tgtcggcgac atccgcggca taccaggggg
      601 cggtgcaggt gtcacagcgc ccgcagtcgg ccgcggtcgg gtcgtccagg cagcgttgca
      661 ggaacgccat ccgacactcg ccggtccgct cgtagtcgag catgagttgc tgctcggcgc
      721 gacgggcggc ggcgactcgt tcatagcgtt cggcgtcgta ggtccacgac tcccccgtcg
      781 cgagccaccc accgctgacc cgccggaccg cgccgtcgac gtcgaggacc ttgagcagca
      841 gttccaaccg agtccggcgg atgtcgacga tggcttccag ggcggcggtc gagagcggcc
      901 gccctgcggc agtgagggcc tcgatcaccg ctcgggcgcg ctcctcggtg ggcatggaga
      961 cgctggcgaa gtaggcccag atgtcgcggt cctccgcgcc gggcatgagc agcacgtcgg
     1021 cccgttcggt ggcccggccc gcgcgaccaa tctgctggta gtaggcgacc ggcgacgagg
     1081 gcgccccgac gtggacgacg aagccgaggt cgggcttgtc gaaacccatc ccgagggccg
     1141 aggtcgcgac cagtgccttc accttgttgt cgcgcagggc gtgctcgagc cgttcccgct
     1201 cggccgggtc ggtgcgaccg gtgtaggccg cgacccgatg gcccgcctgg gcgagggtgg
     1261 tggccaggtc ctcggccgcg gcgacggtga gcgtgtagac gatgccgctg ccgggcaact
     1321 cgcccaggtg cgtgagcagc caaccgagcc gtcccnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnccccg ccgagctcgc gttccacatc cgcgacaacg cgagcattcg
     1501 cggtggccgt agtggcgagc accggagtgc ccggcgggag ggctctcaag aggtcgccga
     1561 tgcgtcgata gtcggggcga aagtcgtggc cccagtcgct gacgcagtgg gcctcatcga
     1621 cgacgagcag gcccgcccgg gcagcgaggt cgggcaactg ctccgcgcgg aacgcgggat
     1681 tgttgagccg ctcggggctc accaggagga cgtcgaccgc atcgtcggcc agggcttggc
     1741 gcacctgggc ccagtcgagg gtgttcgccg aattcatcgt caccgcgcgg atccccgccc
     1801 gggtcgcagc ggcgatctgg tcacgcatga gggacagcag cggcgagacg atgatcgtcg
     1861 gcccggcgcc gttggcccgc agcagcgcgg tcgcgacgaa atagaccgcc gacttgcccc
     1921 accccgtgcg ctggacgacc agcacccgct cgcgatcccg gacgagcgcg agagccgact
     1981 cgagttgtcc ctcgtggaag tcgacatcgg cgcgtcccac gagtcgccgc agcgccgccc
     2041 gcgcgttgcc ttccagatcg tcgtccacac ccgacaccgt atgccgcccc gccgacaacg
     2101 cccgaccctc cccgccccgc ccgagatcgc cgaaattgtc gtcgaattgg tggatgagtc
     2161 ggtcggtacg ccgggttctg ttccgcagca accgtttccg gctgccgcag cgacggtcat
     2221 ccatctcggc cggccattgc tggctggctc tagcgctcta cccgcagact cgggcgggcc
     2281 gccctcaaac gcctgctgtt cgagcttgct ccgggtgggg tttaccgagc cgacctggtc
     2341 acccagatcg ctggtggtct cttacaccac cgtttcaccc ttacctcgcg ggccggcgga
     2401 ccggcatacg aggcggtttg ttttctgtgg cactgtcccg cggatcactc cgggtggccg
     2461 ttagccacca ccctgcccta cggagcccgg acgttcctcg gcacccggct cgcaccggat
     2521 gacgcgaccg cctgaccgac tcatccaccc cgagtgtatg ccgctcggca gcgatgtcag
     2581 aggtgcgccc tagcgtgggc tccatgatcg accacttcgg tatcaactgc agcgacatgc
     2641 ccacggccgc acgcttctat gacggcgtcc tcggggtcct cggccatcga cgagtcatgg
     2701 acttcggtgc ggccatcgga tacgggaccg aggcgcccgt cttctggatc agcaccttcg
     2761 acggcgtggg gcccaaccga gaggtccacg tggcgttcac ggcacctgat gccgccgccg
     2821 tgcgggagtt ccatgccaaa gccctggagt tgggcgccga ggagttgcac gcaccgaggc
     2881 tttggccgga atatcacccc ggctactttg gcgccttcgt gcgggatccc gacggcaaca
     2941 acatcgaggc cgtctgccac gccgctggcc ctactgactg aggccgttcc ggcgcgttcg
     3001 cgcgccctca gaacgggggc gggtcatcag cagggtcgtc ggtatgccgg gtgctcaccg
     3061 catgcgctgc ccaggcgccg gcatggcgcc cgtcgaggta gtcatggggt tcggtgacat
     3121 agctgcgccc ggccagagtg gtccaggtga cgctcgcggt ctcggggtcc tgacgggtcg
     3181 accacaaccc gaaggtcttg agctggtgat gtcgacggtg cttgaccgag aggttggccg
     3241 tcgagctctc accggccggc cagggaacgt cgtggtcgag atcggccccg atcggactga
     3301 cctggcatcc ggggccgcgg catacccgat cgcgcgctgc gacgtgcgtc cgcatggcct
     3361 cggtcggccg gtagcggtga gtcgacacct cgatcagcgc acccgtgcgg tcgtcaacaa
     3421 ccaaccgtcg ccacaccgat ccggccgcat gggcgatctg ccggacgtgt gacgccgaga
     3481 cgaacccaaa cccaggcaac tcgcccggct cgtcatcgca ccccaacaac gttgccaagg
     3541 tgacgatcac gtccacatgg gccgggggcg cagccccgaa cctctggcct gggatgagcg
     3601 gcgcagtacc gaccaccgcg gcgggggcga gggacgcggc gccgatgctc gcgccgtcgc
     3661 caacgggggt cggtccactc tcgggccgat cggacacaac ccgcccgtcg ccgcgcatga
     3721 gcaactcgaa agcgacatcg gaacgcagtt ggtcgagggt cacctcggag taaccaccac
     3781 cggcgcgcaa cccgcgggcc aaagcgtcga tccggctcat taccgaacac acccgggcgg
     3841 cctcgccgac gatccggacc accccggtgc cgtcgtcgaa gagctctgcc gaggtccgac
     3901 gcgcgcgctt cgcacgctcg gcgtgcttgg ctgcggcagc cgggtcatcg gcgcggtgtt
     3961 gggccagcgc gcggcgcaat ctgcgcccca ccagagcatg cgaagcgacc gagccatccc
     4021 ggcacggagc gaccacccgc agggcgacct ctccagcctc ctcggcgtcc agctcgctga
     4081 tctcgtcgca caaccgcgcg accctggcca agtccgcctc ccccgcggcg agcatcgcga
     4141 tgagcccctc ggaccgggcg ggctcagccg tggccagtcg cagccgacgc cagcactccc
     4201 cctcgggcaa tccggtggcg gccatcacct cggcgaccac acccccgcgc accgccgcag
     4261 ccgcctgcgc ccgaagcgcc gccgggccag gcagcgatcc gcgcaccgcg gcacccgccc
     4321 gcgccgcctc gacgaggggg accaaccgct cggccgcctc gcgcgcataa ccggcataca
     4381 gcccgcgcca tgccgacacc gcgagggcat cgagccgcgc cttcagctgt tcgatgagga
     4441 cgatcgctgc cagagcagcg gcctcctgat cggccccgga cagcggggca agcgcctcgt
     4501 ccgggcacag cgcgagcgcc gccgataccg ggtcgggcga gtggatcggc tgctctgaca
     4561 tgtcctgatt ctactcgaac gtctgtccca aaccaagccc aatctattta cacaccaacg
     4621 gttttcgcta agagcgaaca tcgggaaccg caccccaaac caaccccaag agtggccccc
     4681 aaggcacctc gcgaccatcg cggcataccc tcccggtatg ccgcccttcc gcaagtcccg
     4741 cgccgacgcg ccggtcggct acttcgcctg ggaggctgcc gggctgcgtt ggctcggcgc
     4801 cgccacggat gccggcggcg tccccgtcgc cacggttctc caggtcggtc cgacgcacct
     4861 cgatctcgtt cggctcgatc ccgtgccacc cacccgggcc gccgcagcgg ccttcggcgc
     4921 agacctcgcc cgcactcacg accccggcgg cgcggacgtc ggcggcgcgg acgtcggcgg
     4981 cgcgaacccc gccttcggcg ccggcccacc ccactggctc ggcgacggct tcttcggccc
     5041 cactgatcag ccgctcccgc tctccctcac tccgcacttt cgctggtccg ccttctacgc
     5101 cgccgaacgc ctcaccccca tcacgcaggg atgccgcgac gcgggggttt tcgacgcgac
     5161 cgaggccgca ctgctcgacc gattggccgc caccatcgac agacatgaca ccgacgaggc
     5221 acccagccgg atccacggcg acctctggtc gggcaatgtc atctggacgt cagccggggc
     5281 caccctcatc gaccccgccg cccaccatgg tcaccgagag gccgacctgg cgatgctcgc
     5341 gctgttcggg tcgccgcacc ttgacgtgac g
//
LOCUS       DAOMZY010000253         5334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_14479_length_5334_cov_4.642925, whole genome shotgun sequence.
ACCESSION   DAOMZY010000253 DAOMZY010000000
VERSION     DAOMZY010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14479_length_5334_cov_4.642925"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(410..1450)
                     /gene="recA"
                     /locus_tag="PLK64_12930"
     CDS             complement(410..1450)
                     /gene="recA"
                     /locus_tag="PLK64_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPV80699.1"
                     /translation="MATAQDKGKALASVMGQIEKAHGKGAVMRLGDDVRPPIEVIPTG
                     SIALDIALGIGGLPRGRVVEIYGPESSGKTTVALHAVANAQKAGGIAAFIDAEHALDP
                     EYAKNLGVDIDNLLVSQPDTGEQALEIADMLIRSGAIDVIVIDSVAALVPRAEIEGEM
                     GDSHVGLQARLMSQALRKLTGALSTTGTTAIFINQLREKIGVMFGSPETTTGGKALKF
                     YASVRLDVRRIETLKDGSEAVGNRTRVKVVKNKVSPPFKQAEFDIIYGQGISREGGLI
                     DMGVDQGFVRKAGAWYTYDGDQLGQGKENARQFLRDNPDLADELEKRIKEKLGVGPRV
                     DKPAVPDVPVDF"
     gene            complement(1713..2534)
                     /locus_tag="PLK64_12935"
     CDS             complement(1713..2534)
                     /locus_tag="PLK64_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV80700.1"
                     /translation="MSRLRRVPRAVGGALPRPAGAGLARTLVERNILSFRRQWVAFAT
                     GFAEPVFYLFSLGIGLGALVGSVTTDAGHSVSYAVFVAPAMLATSAMNAGVMDSTFNV
                     FFKLKYAKLYDSVLATPMGPRDVAVGEVTWSLLRGGAYAAGFLVVAALAGVLPSWWGL
                     FALPAAVFVAFAFSAVGMFATTYMRSWVDFDYVNLVVQPMFLLSATFFPLSAYPGWAQ
                     WLVEATPLYHGVALCRALCLGEVGVGLLWHAAYLGVMGAVGVVGAARRVDVLLLR"
     gene            complement(2531..3415)
                     /locus_tag="PLK64_12940"
     CDS             complement(2531..3415)
                     /locus_tag="PLK64_12940"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV80701.1"
                     /translation="MDHPPARDERTQPHGIPKRGYAAPAPEPHAILTATRRWVPIVEF
                     HLRVMRRVWRGMLYSRLGTPLLSLLAVGWGVGLLVDRGTAGGVPWHGSVLPYAVFVVP
                     AILASSAMMTGFGESSWPVFGAIKWQGTYHAMLATPMRTRDVLVGHVAMVGGQLAVGA
                     VAFVALAAPFGVWRSWWVLLTLPVATLTGLAFATIMTAVAARSETEGWFTAIFRVVAT
                     PLMLFSGTYFPIESLPAALLPLAWVTPLWHGVEACRALATGLIDVGPLLGHLAALVAF
                     TAVGVVWSARELERRLVR"
     gene            complement(3415..3669)
                     /locus_tag="PLK64_12945"
                     /pseudo
     CDS             complement(3415..3669)
                     /locus_tag="PLK64_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            5030..>5334
                     /locus_tag="PLK64_12950"
     CDS             5030..>5334
                     /locus_tag="PLK64_12950"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPV80702.1"
                     /translation="MAENVADSAPLHPLADYDVLVVGAGFAGATVARELAERGGKRVL
                     ILEARDHIGGNAYDRLDDAGVLIHQYGPHIFHTVDRRVFDYLSRFTTWRPYEHRVQAL
                     "
BASE COUNT          813 a         1918 c         1840 g          763 t
ORIGIN      
        1 gtcgagctgg agcgcaagct ggcccggcgc ggctgcccca cctcggtgag ccggtcgagc
       61 acctcggccg ccacttgcgg gtcgcagccg cgccgggcca gcttgcgctc cagctcgacc
      121 cgactatgcg gccgcagcgc cagcacccgc aacgcgatgc cgtgcgcaac agaccgggga
      181 tcaggctcgg agtcgtcagc gacccgcccg ttggcgaccg tcggggcccg gggcgcacca
      241 gcactcgggg catgtgagtc acgagcactt gggtccggtg gacctgccgg ggccgatagc
      301 tcagggccct gccgagcggc cgcctcggcg gctcgtaggg ctgccaacgc cgcagcctgg
      361 cgtgccgcgc ccggggagtc cgggcgcggc ataccgtcag gcgtcatgct cagaagtcga
      421 ccggaacgtc gggcacggcc ggcttgtcga cgcgcggccc gacgccgagc ttctccttga
      481 tccgcttctc gagctcgtcg gccaggtcgg ggttgtcgcg gaggaactgg cgggcgttct
      541 ccttgccctg gccgagctgg tcgccgtcgt aggtgtacca agcgccggcc ttgcgcacga
      601 agccctggtc aacgcccatg tcgatcaggc caccctcgcg ggagatgccc tggccataga
      661 tgatgtcgaa ctcggcctgc ttgaacggcg ggctcacctt gttcttgacg accttgactc
      721 gggtgcggtt gccgaccgcc tctgaaccgt ccttgagcgt ctcgatccgt cggacgtcca
      781 gccgcacgga ggcgtagaac ttcaacgcct taccgccagt agtggtttcg ggacttccga
      841 acatcacacc gatcttctcg cgcaactggt tgatgaagat cgccgtggtc ccggtggtgc
      901 tcagggcgcc agtcaacttg cgcaacgcct gcgacatgag gcgggcctgg aggccgacgt
      961 ggctgtcgcc catctcgccc tcgatctcgg cgcggggcac gagcgccgca acggagtcga
     1021 tgacgatgac atcgatcgcg ccggagcgga tgagcatgtc ggcgatctcc agcgcctgct
     1081 cccccgtgtc gggctggctg accagcaggt tgtcgatgtc gacgccgagg ttcttggcgt
     1141 actccgggtc gagggcgtgc tcggcgtcga tgaaggccgc gatgccgccc gccttctggg
     1201 cgttcgccac ggcgtgcaga gcgaccgtag tttttccact gctctcgggt ccgtagatct
     1261 cgacgacccg gccgcgcggc agaccgccga tcccaagggc gatatcgagg gcgatcgacc
     1321 cggtggggat cacctcgatg ggcggtcgga cgtcgtcacc cagacgcatc acggcaccct
     1381 tgccatgggc cttctcgatc tgccccatga cacttgccag ggccttgccc ttgtcctgcg
     1441 ctgtggccat gccacccacc atccttcgtc tcgagagtcg gccgtccata ggcgacccgc
     1501 gcgacgggcc ggtcactccc ccggtcgatg tgtcgaccgg gcaagtccag cgcccgtgtt
     1561 gtcgagtcgg acgttaggcg aacccaccga cagacctcag cccacggcct acagcctgtg
     1621 gacaatggga cgacggtgcg gacacctgtg gacaaacatt accgaacgga tgttcgagtg
     1681 ccactcgaca cgccgggccg gacaaccatc cctcagcgca gcagcaggac gtcgacgcga
     1741 cgagccgcac cgacgacacc gacggcgccc atgacgccga ggtaggcggc gtgccacagc
     1801 aggccgaccc ccacctcgcc caggcacagc gcccggcaca gggcgacccc gtgatagagc
     1861 ggggtcgcct cgacgagcca ttgggcccac cccggatagg ccgacaacgg gaagaacgtc
     1921 gccgagagca ggaacatcgg ttggaccacg aggttcacgt agtcgaagtc gacccacgag
     1981 cgcatgtagg tcgtcgcgaa catgccgacc gcggagaacg cgaacgccac gaaaaccgct
     2041 gcgggcagcg cgaacagccc ccaccaggac ggcaagacgc cggccaacgc ggcgacgacg
     2101 aggaaccctg cggcatacgc cccgccccgc agcaacgacc aggtgacctc ccccaccgcg
     2161 acgtcgcgcg gccccatggg ggtggccagg accgagtcgt agagcttcgc gtacttcaac
     2221 ttgaagaaga cgttgaaggt cgagtccatg acgcccgcgt tcatcgccga cgtcgcgagc
     2281 atcgcggggg cgacgaacac ggcatacgaa acgctgtgac ctgcgtcggt ggtcactgag
     2341 ccgaccagcg cccccaaccc gatgcccagg gagaacaggt agaagaccgg ctcggcgaac
     2401 ccggtggcga acgccaccca ctggcgccgg aaggacagga tgttgcgttc gaccagggtg
     2461 cgcgccagac ccgcgccagc cggccgcggc agcgcccctc cgacggcccg gggcacgcgc
     2521 ctgaggcggc tcatcggacc aaccgccgtt ccaactcacg ggccgaccac acgacgccca
     2581 cggcggtgaa cgccaccagc gcggccaaat gcccgagcag cgggcccacg tcgatcagcc
     2641 cggtcgccag tgctcggcag gcctcgacgc catgccatag cggcgtcacc caggccaacg
     2701 gcaggagggc ggcggggagc gactcgatgg ggaaataggt gcctgaaaac agcatcaacg
     2761 gcgttgcgac gacccgaaag atcgcggtga accagccttc ggtctcactg cgggccgcca
     2821 ccgccgtcat gatcgtcgcg aaggccagtc ctgtgagggt cgccaccgga agggtgagca
     2881 gcacccacca ggaccgccac acaccgaacg gtgccgcgag cgcaacgaac gcaaccgccc
     2941 ccaccgccag ttgcccgccc accatggcga cgtgccccac gaggacgtcg cgggttcgca
     3001 tcggggtcgc cagcatcgcg tggtaggtgc cctgccactt gatcgccccg aacaccggcc
     3061 aactggactc cccgaaaccg gtcatcatcg ccgagctcgc caggatcgcg gggacgacga
     3121 acacggcata cgggaggacg gagccgtgcc acggcacccc tccggcagtg ccgcggtcga
     3181 cgagcagccc cacgccccac ccgacggcca gcagcgagag cagcggggtc cccagccggc
     3241 tgtagagcat cccgcgccag acccgccgca tcacccgcag gtgaaactcg acgatcggca
     3301 cccaccgccg ggtggcagtg aggatcgcgt gcggctccgg cgcaggggcg gcataccccc
     3361 ttttcggtat gccgtgcggc tgggtccgct cgtcgcgcgc gggtgggtgg tccatcagtc
     3421 caccagcgtc cgaccggtga ggtgcaggaa gacgtcctcc agcgacgagc ggcgcacaag
     3481 agcggacaac ggacggatcc cgcgggcgtg ggcagcagcg agggcgccgt cgccgtcgtc
     3541 cgcgtagacg agcaggcggt cgggcagcac ctcgacccgt tcgccgatgc cctgcagcga
     3601 cggcacgtgg atgccgttgt cgtcgtcagc gaagcgcaac tcgagaacct cgcgggtcgc
     3661 gtgggtctgc gcgaaggctt cgcgctgcat ctgctcgagt tcgccggtgg tccgttcgcg
     3721 gacgacgcgg gcgaagccct cggcatccgc gagccgactc tcggcgcggg ccagctgcgc
     3781 tgcgcagcgg accctggtct cctccagcag ttggttggcc tcgttggtcg catgcaggcg
     3841 ggtctgctcg gcgtcggcct gcgccgcctc gaggacggcg gtcgccttgg cccgcacctg
     3901 gagcgcctcg gccctggtgt tggcggcctc gccctccacc cgtgcgctga gctgggtgat
     3961 ggccgcacgc agttgggtgc ggatgtcgcg gacgtgagcg gcctgttcgt cgtgggtgcg
     4021 ggccttcgcg aggcggatct cggtgtcggc ctggtccatc agttgctgga ccgtcgcctg
     4081 cgtccaggcg agctgctccc cggcggagcc gatcgtctcg gcggcctggc gttgcgcgtc
     4141 ctcgaccacg gagcgtgcgt gggcgtccgc ctgctcccgg agggcgaccc gctcggactc
     4201 ggcccgggcg gcggcctcct cggcggccga ccgcacgtcg tccgcgtgct gctgcgcctc
     4261 tgcgatgatc cgggccgcct cctgggcggt tcgctcgacg atgccggcgg cgtgcgtctc
     4321 gaggcgcgcg cgatcgagtt cacgttgcgc gtcgtgctcc gtccgcctcc gttcgaggtc
     4381 cgcctggacg gcccgagcct cctcggcctg ctgcgcgcgc tgggcagccc gttcgtcggc
     4441 ggccgcctgt cgggtccgac gggcctccga gacggcctcc gccatcagat ggccggcgtc
     4501 ggcgcgggcc gtcgcgagca ggtcagccgc cgtccgctcg gcggcggcgc tgacggcggc
     4561 accctgcgcg gcggcgtccg agagcatgtc gcgggcgtcc gcgcgcgcgg actccaggac
     4621 cgtggaggcc tcgcggacga tcccgtcgac ggttttgctg cgttcctcgg cgcgcaggag
     4681 gaggtcctcg gcccggcgtc gcttgccctc ggcgtccgag atcagttcgg cggattcggc
     4741 cagcgcctcc tggcgaaggc ggtccgccaa cacctgggcg atctcgagga cgtcggctgc
     4801 gcgtgcccta gtgtcgtcgc gaggcggcag ccccgcggtc ttctggtcca cgcgcatgat
     4861 cgccattttg gggccgagga gacctgtccg caaagccgcg gtcaagagtt accgaaccgc
     4921 cgcccaccgg cccggcgttg cgcgggtcgt gacgcggcac ggtagcctcg atgggtccga
     4981 agcgcacggg tccgcgaccc ggcccgccga ttcgagggag acacccgctg tggctgagaa
     5041 cgtcgccgac tccgcaccgt tgcacccgct ggccgactat gacgtgctgg tcgtcggggc
     5101 aggtttcgcg ggggcgaccg tcgcccgcga gctcgccgag cgtgggggca agcgtgtcct
     5161 catcctggag gcacgcgacc acatcggcgg caacgcctac gaccggctcg acgacgcggg
     5221 cgtcctgatc caccagtacg gaccgcacat cttccacacc gtcgaccggc gcgtgttcga
     5281 ctacctgtcg cgcttcacca cctggcgccc ctacgagcat cgcgtgcagg ccct
//
LOCUS       DAOMZY010000254         5223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_14910_length_5223_cov_5.918344, whole genome shotgun sequence.
ACCESSION   DAOMZY010000254 DAOMZY010000000
VERSION     DAOMZY010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14910_length_5223_cov_5.918344"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2155
                     /locus_tag="PLK64_12955"
     CDS             <1..2155
                     /locus_tag="PLK64_12955"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HPV80703.1"
                     /translation="RKHPRVQAGTGTGKSLAYLVPAVQHALDTGKPSVVATATLALQA
                     QIVDRDLPRLATALAPLLGRSPTFAMVKGRRNYLCLHKLEGGFPDDDALLDVGEVDRA
                     VGRLGQEVIRLRAWAEITESGDRDELVPGVSDRAWRQVSVDAHECLGGKCPLVTACFV
                     ERSRAAAAEVDVIVTNHSFMAIDSFEGRQMLPEHDLLVVDEAHELVERVTSTITDELT
                     AGMVSGAARRAGKRSERSDDATGAGDLLGSVLDGMPEGRVLSLPDALVLALSRVRDVA
                     RAMQSELKPEAGAEADGALQVARAAVDEVFENAERILEGRELDVVWLSHEPRRGAVLR
                     VAPMSVAMLVRDKVLADRTVVLTSATLELGGSFDAVAGTMGLRGEGAPGWTGVDVGSP
                     FDYPRQAIAYVAAHLPPPGRDGTPTEAMDELEALVLAAGGRTLGLFSSMRAAQAASEE
                     LRDRFSRQGVAIEILCQGEDQISTLVRQFARDSTTCLFGTLSLWQGVDVPGSSCQLVV
                     IDRIPFPRPDDPLASARTQAIARMGGNGFMAVSATHAALRLAQGAGRLVRRSDDRGVV
                     AFLDSRMTTARYAGFLQRSLPPFWPTTDREKVLAALRRLDEVAPPPLPVAEPTARGLG
                     GAPAAYPMGEEGGEPASDPRPVAGPPPVAPSPRTAVTGGHAWTSAQDAELRDAAEAGI
                     DLDELVEHFELEIDVIQARLAQLGLEIGEPTFGFE"
     gene            2368..2871
                     /locus_tag="PLK64_12960"
     CDS             2368..2871
                     /locus_tag="PLK64_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HPV80704.1"
                     /translation="MTTDSDFKALVRARMALTGQTYTAARADVIAERGPAADPHQPGP
                     TAPETDLQRSADDAFYGKTVRSFFDGDRLRAVPAKRKARVVVLLELLKRFEAGRTYAE
                     PEVNALLRQAHDDVAFLRRELVDYRYLERADGRYWVTTQTPERDANEAQEVPTAEAAL
                     LEAMRRS"
     gene            complement(2874..4226)
                     /locus_tag="PLK64_12965"
     CDS             complement(2874..4226)
                     /locus_tag="PLK64_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaminase"
                     /protein_id="HPV80705.1"
                     /translation="MIDRTRLAALMSSEQDRFAAAHPRSAAAYGRADHLFGRVPMTWM
                     NKKAGGFPLYFERGRGSRVWDIDGNDYIDFALGDTGAMAGHSPEPVVAAIRRRVDHLG
                     GLTTMLPTEDAEWVGAELSRRFGMDVWSFSLTATDANRWAIRLVRALTGRPKILVNSY
                     CYHGSVDESLIVVGEDGEGTSRPGNVGSPVDVTMTSRVAEFNDIEGLEKQLAHGDVAA
                     VLMEPALTNIGIVLPDPGYLDAVREVTRRHGTLLIIDETHTFSAGPGGMTQRDRLSPD
                     VVVIGKAIGGGIPTGAYGLSAELAARALSRTDLDLVDMGGVGGTLAGNPLSVAAMRAT
                     LEHVLTDDAFTGMIDTATVFVDGVQSLIDEHHLPWSINQLGARGEYRFATPYPRNGTQ
                     AAASADGELEDYLHLYLVNRGILLTPFHNMALMAPTTTVADVQRHHEVFAEALGTLPR
                     "
     gene            4238..>5223
                     /gene="holA"
                     /locus_tag="PLK64_12970"
     CDS             4238..>5223
                     /gene="holA"
                     /locus_tag="PLK64_12970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPV80706.1"
                     /translation="MPEVSPLRGRLAGVSVPPFVLITGSEGLLAERALASVLAELRHT
                     EPDLEAIRVAAAAYEGGVLGLHASPSLFGGAKAVVVEDFDEGGEELQADVLALVAAPA
                     PDLTLVVMHKSGQRGKKALDALKAARAQVIECPPIKTDRDKADFVTHEFRRQGRKVTP
                     GAVQALVEAVGKDLRELASACSQLVADTVGVVDERVVETYHAGKVEASGFRVADAALA
                     GQTSEALRLLRHAIATGVDPVPIVAVLASQLRTLVKVGAAGRGSSAGIAKTLGMAPWQ
                     VDKARRSISGWDGPGLGVAIQAVAAADVEVKGGGRDPVYAVERAILSIAAARR"
BASE COUNT          734 a         1790 c         1910 g          789 t
ORIGIN      
        1 ccggaagcac ccgcgcgtcc aggccgggac gggcaccggc aagtcgctgg cctacctcgt
       61 gcccgcggtc cagcacgccc tcgacaccgg caagccctcc gttgtcgcca ccgccactct
      121 ggcgctgcag gcccagatcg tcgaccgcga cctgccccgg ctggcgaccg ccttggcgcc
      181 gctgctgggg cgctcgccga ccttcgccat ggtcaagggc cggcgcaact atctgtgtct
      241 gcacaagctc gaaggcggct tccccgacga cgacgccctg cttgacgtcg gcgaggtcga
      301 ccgtgcggtg gggcggctgg ggcaggaggt catccggctg cgcgcgtggg cggagatcac
      361 cgagtccggc gaccgcgacg aactcgtccc gggcgtgtcc gaccgggcct ggcggcaggt
      421 gtcggtcgac gcccacgagt gcctgggcgg gaagtgtccg ctggtcaccg cctgcttcgt
      481 ggagcgctcg cgggccgcgg cggccgaggt ggacgtcatc gtcaccaacc actcgttcat
      541 ggcgatcgac tcgttcgaag gtcgccagat gttgcccgaa cacgatctgc tcgtggtcga
      601 cgaggcgcac gagttggtcg agcgggtcac ctcgacgatc accgacgagc tgaccgcggg
      661 catggtgagc ggcgcggccc ggagggccgg caagcgcagc gagcggtccg acgacgcgac
      721 cggggcgggg gacctgctcg ggtcggtcct tgacggtatg ccggagggcc gggttctcag
      781 cctgcccgat gcgctcgtcc tggccctgag ccgggtccgg gatgtcgccc gggcgatgca
      841 gagtgagctc aagcctgagg ccggggccga ggcggacggg gcgctgcagg tcgcgcgggc
      901 cgcggtcgac gaggtgttcg agaacgccga gcggatcctc gagggccgcg agctcgacgt
      961 cgtctggctc tcccacgagc cgcgccgggg cgccgtgctg cgggtcgcgc cgatgagcgt
     1021 ggcgatgctg gtgcgcgaca aagtgctcgc ggaccggacc gtcgtgctga cctcggcgac
     1081 gctggagttg gggggatcct tcgacgcggt ggccgggacg atggggctgc gcggcgaggg
     1141 tgcgcccggg tggaccggcg tcgatgtcgg ctcgcccttc gactacccgc gccaggcgat
     1201 cgcctatgtc gcggcccacc tgccgccacc cggtcgggac ggcaccccga cggaggcgat
     1261 ggacgagctg gaggccctgg tgctggccgc cggcggccgg acgctcgggc tgttctcctc
     1321 gatgcgggcc gcccaggcgg cctcggagga gctgcgcgac cgcttctccc gtcagggggt
     1381 ggccatcgag atcctgtgcc agggtgaaga ccagatctcc actctggtac ggcaattcgc
     1441 acgtgactcg acgacgtgcc tgttcgggac cttgagtctg tggcagggcg tcgacgtgcc
     1501 ggggtcgtcg tgccagttgg tcgtcatcga ccggatcccc ttcccgcgac cggatgaccc
     1561 attggcctcg gcacggactc aggcgatcgc ccggatgggc ggcaacgggt tcatggcagt
     1621 gtcggccacc catgcggcgt tgcgattggc gcaaggcgcc ggccggcttg tccgccgctc
     1681 cgacgaccgg ggcgttgtcg ccttcctcga ctcccggatg accaccgcga ggtatgccgg
     1741 cttcctccaa cgctcgctgc ctcccttctg gccgacgacc gatcgggaga aggtgctggc
     1801 tgccctgcgg cggttggacg aggtcgctcc accgccgttg ccggtcgccg agcccacggc
     1861 ccgggggctg ggaggtgcgc ccgcggcata cccgatgggg gaggagggcg gcgagccggc
     1921 gagcgatccg cgcccagtgg ccggaccccc gcccgtcgcg ccctcaccgc ggacggctgt
     1981 caccggcggt catgcctgga cgtcggcgca ggatgccgag ctgcgagacg ctgccgaggc
     2041 ggggatcgac ctggacgagt tggtcgagca cttcgagttg gagatcgacg tcatccaggc
     2101 gcggctggcg caactggggc tggaaatcgg ggagccgacc ttcggtttcg agtgacgcgc
     2161 tgcgcttcgg cgcaactcgg ttgacctggg cggccgtccg atgccaggct gacccgagcg
     2221 gcgtaacctg acgcccagcc ggtgccggcc ccagcctgcg cacatcacgg cgaaggggag
     2281 cacatcctcg ggctgacatg ctgtcgtcgc gttcgcccca caggctgcgg cgggctgggg
     2341 ccgtgcacag ccggagagcg attcccgatg accaccgatt ccgatttcaa ggccctcgtc
     2401 cgcgcccgga tggccctgac cggccagacc tacaccgccg cccgcgccga cgtgatcgcc
     2461 gaacgtggcc cggcagcgga ccctcatcag ccgggtccca ccgcacccga gaccgatctg
     2521 cagcgatcgg cggacgacgc gttctacggc aagactgtgc ggtcgttctt cgacggcgat
     2581 cggctgcgcg ccgtcccggc caagcgcaag gcgagggtcg tcgtgctcct ggagctgctc
     2641 aaacgcttcg aggcaggccg gacctacgcc gagccggagg tcaacgcgct gctgcgacag
     2701 gcccacgacg acgtggcctt cctgcgtcgg gagttggtcg actaccgcta tctcgagcgc
     2761 gcggacggcc gctactgggt caccacccag acaccggagc gggacgccaa cgaggcgcag
     2821 gaggtgccga ccgccgaggc ggccctcctg gaggcgatgc gccggtccta gcgtcagcgg
     2881 ggcagggtgc ccaaggcctc ggcaaagacc tcgtggtgac gctggacgtc ggcaaccgtc
     2941 gtcgtcggag ccatgagcgc catgttgtgg aatggcgtta ggaggatccc gcggttgacc
     3001 aggtagaggt gcagatagtc ctcgagctca ccgtcggccg atgccgccgc ctgcgttccg
     3061 ttgcgtgggt agggcgtcgc gaagcggtac tccccgcggg cgcccagctg gttgatcgac
     3121 cacggcaggt ggtgctcgtc gatgagcgac tgcactccgt cgacgaacac cgttgcggtg
     3181 tcgatcatcc cggtgaacgc gtcgtcggtc agcacgtgct cgagggtcgc acgcatcgcc
     3241 gccaccgaca gcgggttgcc ggcgagggtg ccgcccaccc cgcccatatc gaccaggtcg
     3301 aggtcggtcc gggacagggc cctcgcggcc aactcggcgg acaggccata ggcccccgtc
     3361 ggtatgccgc cgccgatcgc cttcccgatg acgacgacgt cgggggagag ccgatcccgc
     3421 tgggtcatcc cgcccggccc tgccgagaag gtatgcgtct cgtcgatgat gagcagggtg
     3481 ccgtgtcgac gggtcacctc gcggacggcg tcgaggtagc ccgggtccgg caggacgatg
     3541 ccgatgttgg tcagcgcagg ctccatcaac accgccgcca catcaccgtg agccagttgc
     3601 ttctccaggc cctcgatgtc gttgaactcg gccacccggc tggtcatcgt cacatcgacc
     3661 ggcgaaccga cgttgcccgg ccggctggtg ccctcgccat cttcgccgac gacgatgagc
     3721 gactcgtcga ccgacccgtg gtagcagtag ctgttgacga ggatcttcgg tcgcccggtg
     3781 agggctcgca ccagccggat cgcccaacgg ttggcgtcgg tcgcggtgag cgagaagctc
     3841 cagacgtcca tcccgaaccg gcgcgacaac tcggcgccca cccattcggc atcctcggtg
     3901 gggagcatcg tcgtcagccc gccgaggtga tcgactcgcc gtcgaatggc ggcaacgacc
     3961 ggctcggggg agtgccccgc catcgcgccg gtgtcaccca gggcgaagtc gatgtagtcg
     4021 ttcccgtcga tgtcccacac ccggctcccg cggccgcgtt cgaagtagag ggggaagccg
     4081 cccgctttct tgttcatcca ggtcatcggc acccgcccga agaggtggtc agcccggccg
     4141 tatgccgccg cggagcgcgg atgggcggcg gcgaagcggt cctgttcgga agacatgagg
     4201 gcggccaggc gggtgcggtc gatcatggga ggccatcatg cccgaggtgt caccgctgcg
     4261 tgggaggctt gcgggcgtga gtgttccgcc gttcgtcctc atcaccggct ctgaagggct
     4321 gctcgccgag cgcgccctgg cctcggtgct cgccgagctg cgccacaccg agccggacct
     4381 cgaagcgatc cgggtggctg ctgcggcata cgagggcggg gttctgggcc tgcatgcgag
     4441 cccgtcgctc ttcggcgggg cgaaggcggt cgtcgtcgag gacttcgacg agggcggcga
     4501 ggagttgcag gccgacgtcc tcgcgttggt cgccgcaccg gcacccgacc tcacgctcgt
     4561 cgtcatgcac aagtccggtc agcgcggcaa gaaggctctc gacgcgttga aggcggccag
     4621 ggctcaggtc atcgagtgcc cgccgatcaa gaccgaccga gacaaggccg acttcgtcac
     4681 ccatgagttc cgcaggcagg gtcgcaaggt cacgccgggt gccgttcagg cgctggtcga
     4741 ggcggtcggc aaggatctgc gcgagctcgc gtcggcctgc agccaactgg tcgccgacac
     4801 ggtcggggtc gtcgatgagc gggtggtcga gacctatcac gcgggcaagg tggaggcgag
     4861 cggcttccgg gtcgccgacg ccgctctggc cggtcagacg agcgaggcac tgcggctgct
     4921 gcgccacgcg atcgcgaccg gcgtcgaccc ggtgccgatc gtcgccgtcc tcgcctcgca
     4981 attgcgcacc ctggtcaagg tgggtgcggc cgggcgcggg tcgtcggccg ggatcgccaa
     5041 aaccctcggc atggctccct ggcaggtcga caaggcccgc cgatcgattt ccggatggga
     5101 cgggcccggg ttgggcgtcg ccatccaggc ggtcgcggcc gccgacgtcg aggtcaaggg
     5161 tggcggccgc gacccggtgt atgccgtgga gcgcgccatc ctctccatcg ccgccgcccg
     5221 tcg
//
LOCUS       DAOMZY010000255         5210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_14961_length_5210_cov_5.806596, whole genome shotgun sequence.
ACCESSION   DAOMZY010000255 DAOMZY010000000
VERSION     DAOMZY010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14961_length_5210_cov_5.806596"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /gene="ahpC"
                     /locus_tag="PLK64_12975"
     CDS             complement(<1..481)
                     /gene="ahpC"
                     /locus_tag="PLK64_12975"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HPV80707.1"
                     /translation="MALIGSEIKPFTATAFKSGSFVVVSEHDLKGKWSVVFFYPADFT
                     FVCPTELGDMADHYAELQAMGVEVFSVSTDTHFTHKAWHDSSDTIGKIDYFMVGDPTG
                     EITTNFKVMREGQGLADRGTFVVDPDGIIQVMEVTCEGVGRNAAELVRKVKAAQYVRN
                     "
     gene            complement(650..1411)
                     /locus_tag="PLK64_12980"
     CDS             complement(650..1411)
                     /locus_tag="PLK64_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80708.1"
                     /translation="MKSVLMCATVLVAAVSLAGCGGTAAPAVATATTTPAASTSAAST
                     APTTNAPPAADGIDGTGIAAKMVMALVKAGSGRYDMVSSGAAGGQQVTMTAKSEFVVV
                     NGAMDSKATMSVAGGSMEVVSVGGVMYMKGLIKSKDGKPWVKIDPNGTDEFSKRMAPQ
                     LKSAADLRSQVEVYEGSKATLIGTDAGVRHYRLTGVSSAPAQPFDIYLDASDLPVKVT
                     TKSDSMEMTITYSGFGDAVTVTAPPAAQVGPPPAM"
     gene            complement(1550..2263)
                     /gene="rplA"
                     /locus_tag="PLK64_12985"
     CDS             complement(1550..2263)
                     /gene="rplA"
                     /locus_tag="PLK64_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPV80709.1"
                     /translation="MKRSKAYRAAVDKMTPGKVYGPLEAVRLAKATSATKYDATVEVA
                     LRLGVDPRKADQMVRGTVNLPHGTGKTARVLVFANADKAEAARAAGADFVGADDLIEK
                     ITGGWLDFDAVVATPDMMGKVGRLGKVLGPRGLMPNPKTGTVTMDVAKAVTDIKGGKI
                     EFRVDKHGNLHFIIGKTSFDERALVENYSAAIDEVLRLKPSASKGRYIEKATMATTMG
                     PGIPLDFTKTRNLLAEDEA"
     gene            complement(2379..2816)
                     /gene="rplK"
                     /locus_tag="PLK64_12990"
     CDS             complement(2379..2816)
                     /gene="rplK"
                     /locus_tag="PLK64_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010058817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPV80710.1"
                     /translation="MPPKKKKITGFIKLQINAGAATPAPPVGPALGQHGVNIMEFVKA
                     YNAATEHQRGNVIPVEITVYEDRSFTFVTKTPPAAELIKKAAGIAKGSSEPHKTKVAK
                     LTADQVREIAQQKLEDLNANDVEMAARIIAGTARSMGVQTEGY"
     gene            complement(2905..3705)
                     /gene="nusG"
                     /locus_tag="PLK64_12995"
     CDS             complement(2905..3705)
                     /gene="nusG"
                     /locus_tag="PLK64_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HPV80711.1"
                     /translation="MADQDEVLDIEADETHLDDVEVVEVDGADADLVVDEADADVTAD
                     ADDAESSDEAESSEEAAEVIDPVAEFKATLRAKPGEWFVVHSYAGYENRVKANLETRI
                     ISLNMEDYIFEIDVPMEEVTEIKNGQKKLVRRVRMPGYVLVRMDLTDASWGAVRHTPG
                     VTGFVGNAHQPIPLTLDEVYRMLAPDLAPAAGPAGAGAVGTGSKPKEVRVVDFEIGES
                     VTVMEGPFETLPATISEINPDTQKLKVLVSIFGRETPVEVSFGQVAKI"
     gene            complement(3786..4055)
                     /gene="secE"
                     /locus_tag="PLK64_13000"
     CDS             complement(3786..4055)
                     /gene="secE"
                     /locus_tag="PLK64_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPV80712.1"
                     /translation="MSESSTARTGSRAVRQRPGDGNPFSRFFAALTLFIRQILDELRK
                     VVRPTGSELLNYTVVVILFVVAIMGIVAGLDVVLQRLVLWVFAGS"
     gene            complement(4091..4163)
                     /locus_tag="PLK64_13005"
     tRNA            complement(4091..4163)
                     /locus_tag="PLK64_13005"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4128..4130),aa:Trp,seq:cca)
     gene            4392..>5210
                     /locus_tag="PLK64_13010"
     CDS             4392..>5210
                     /locus_tag="PLK64_13010"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015937870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPV80713.1"
                     /translation="MVPRRVSQRIGAIAESATLAVDAKAKALKAQGRPVIGFGAGEPD
                     FPTPDYIVEAAIAAARNPANHRYTPAAGLPELREAIAAKTLRDSGYAVPATAVLVTNG
                     GKQAVYQAFASIVDPGDEVLLPAPYWTTYPEAIALAGGRAVEVFAGEDQGYLVTVEQL
                     EAARTENTKALLFCSPSNPTGAVYSPEQVRAIGRWAHDHGIWVITDEIYEHLTYDGVR
                     AASIVVEVPELADTCIILNGVAKTYAMTGWRVGWMIGPKDVIAAATNLQSHLTSN"
BASE COUNT          841 a         1723 c         1729 g          917 t
ORIGIN      
        1 ggttgcggac gtactgggcg gccttgacct tgcgcacgag ctcggcggcg ttgcgcccga
       61 ctccctcgca ggtgacctcc atgacctgga tgatcccgtc ggggtcgacg acgaaggtgc
      121 cgcggtcggc caggccctgg ccctcacgca tgaccttgaa gttggtcgtg atctcgccgg
      181 tggggtcgcc caccatgaag tagtcgatct tgccgatcgt gtcggacgag tcgtgccagg
      241 ccttgtgggt gaagtgcgtg tcggtcgaca cggagaagac ctcgacaccc atggcctgga
      301 gctcggcgta gtggtcggcc atgtcgccga gctcggtcgg gcacacgaag gtgaagtcgg
      361 ccgggtagaa gaacaccacg gaccacttgc ccttgaggtc gtgctcgctg acgacgacga
      421 acgaaccgct cttaaaggcg gtggcggtga acggcttgat ctcggacccg atgagagcca
      481 tctgtgggta cacctcgctg gttcggtacg gaaaactgat gatagtcaat ctatatcaat
      541 taacgtctcg acccaagtca tcatcaatcg cctgtgccta tgctgcccat cgtcccgatc
      601 aatgaagggt gcccttgctc gcgaccatcc cccgtgcccg ggggctcgat cacatcgccg
      661 gaggcgggcc cacctgcgcg gccggcggcg ccgtcaccgt gaccgcatcg ccgaagccac
      721 tgtaggtgat cgtcatctcc attgagtccg acttcgtcgt caccttgacc ggcaagtccg
      781 atgcgtcgag gtagatgtcg aacggctggg cgggcgcact cgacacgccc gtgaggcggt
      841 agtgccggac tccggcgtcg gtgccgatca gggtcgcctt ggacccttcg tagacctcga
      901 cctgtgagcg caggtcagcg gccgacttga gttgcggcgc catccgcttg ctgaactcat
      961 cggtcccgtt ggggtcgatc ttcacccagg gcttgccgtc cttggacttg atcaggccct
     1021 tcatgtacat cacgccgccg acgctgacga cctccatcga gccaccggcg acgctcatcg
     1081 tcgccttcga gtccatggcg ccgttgacga cgacgaactc gctcttggcg gtcatcgtca
     1141 cctgctggcc accggccgcg cccgacgaca ccatgtcgta cctgccgctg ccggccttga
     1201 ccagggccat gaccatcttc gcggcgatgc cagtgccgtc gatgccatcg gcggctgggg
     1261 gggcgttcgt ggtcggcgca gtcgatgcgg cgctggtgct cgccgccggg gtcgtggtcg
     1321 ccgtggccac cgcgggtgct gcggttccgc cacaaccggc caacgagact gctgccacga
     1381 gaactgtggc gcacatgaga acggacttca cggcataccc cctgaggaat gtgggacgac
     1441 aactgatcgg tcaccaccct aacaacgctg gccgaccttc gctccacggg gccaaacgaa
     1501 ggcgcccggg acgaggttcc gtccgcgggc gccatcgggg cgagtgaggt caggcctcgt
     1561 cctcggccag gaggttgcgg gtcttggtga agtccagcgg gatgccgggg cccatcgtcg
     1621 tggccatcgt ggccttctcg atgtaacggc ccttggaggc cgacggcttg agtcgcagga
     1681 cctcgtcgat ggctgccgag tagttctcca ccagggcgcg ctcgtcgaac gatgtcttgc
     1741 cgatgatgaa gtgcaggttg ccgtgcttgt cgacgcggaa ctcgatcttg ccgcccttga
     1801 tgtcggtgac ggccttggcg acgtccatgg tcacggtgcc cgtcttaggg ttgggcatga
     1861 ggccacgcgg gccgaggacc ttaccgaggc gaccgacctt gcccatcatg tcgggggtcg
     1921 cgacgacggc gtcgaagtcc agccagccac cggtgatctt ctcgatcagg tcgtcggcgc
     1981 cgacgaaatc ggcacccgcg gcgcgggcgg cctcggcctt gtcggcgttg gcgaagacga
     2041 ggacccgggc ggtcttgccc gtgccgtggg gcaggttgac ggtgccacgg accatctggt
     2101 cagccttacg ggggtcgacc ccgaggcgga gggcgacctc gacggtcgcg tcgtacttcg
     2161 tcgcggacgt cgccttcgcg agacggacgg cctcaagcgg gccgtacacc ttgcccgggg
     2221 tcatcttgtc aacggcggcc cggtaggcct tgctgcgctt cacttctgct ccttcaaaac
     2281 ggtggtcgtg caactggagt tgtggtctcg cgagccgcgc tcggctctcc cacacacggc
     2341 atacccaggt ggtatgccgt gtcggcgccg gccgagggtc agtaaccctc ggtctggaca
     2401 cccatcgagc gagcggtgcc ggcgatgatc cgggccgcca tctcgacgtc gttggcgttg
     2461 aggtcttcaa gcttctgctg ggcgatctcg cggacctggt cggcggtgag cttggcgacc
     2521 ttggtcttgt gcggctcgct ggagcccttg gcgatcccgg cggccttctt gatcagctcg
     2581 gcggccggcg gcgtcttggt gacgaaggtg aacgagcggt cttcgtagac cgtgatctcg
     2641 accgggatga cgttgccgcg ctggtgttcg gtggccgcgt tgtaggcctt gacgaactcc
     2701 atgatgttga cgccgtgctg acccagggcc gggccgacgg gcggggccgg ggtggcagca
     2761 ccggcgttga tctgcaactt gatgaagccg gtgatcttct tcttcttcgg gggcattgct
     2821 gttctccttg atgtttaggc cgacgccttg ggcgatgacc cggttgcagt gccgaccggc
     2881 taatgcacga tcggcgggac tcgatcagat cttggcgacc tgaccgaagg acacctcgac
     2941 cggggtctcc cggccgaaaa tcgagacgag caccttgagc ttctgggtgt cggggttgat
     3001 ctcggagatc gtcgccggaa gggtctcgaa cggaccttcc atgacggtga cggactcgcc
     3061 gatctcgaag tcgacgaccc ggacctcctt gggcttgctg ccggtgccca ccgcgcctgc
     3121 gcccgccggg ccggccgcgg gggccaggtc gggggccagc atgcggtaga cctcgtcgag
     3181 ggtcagcggg atcggctggt gcgcgttgcc gacgaagccg gtgacaccag gggtatgccg
     3241 cacggcgccc cacgaggcgt cggtgagatc catccgcacg aggacgtaac cgggcatccg
     3301 aacgcggcgg acgagcttct tctggccgtt cttgatctcg gtcacctcct ccatggggac
     3361 gtcgatctcg aagatgtagt cctccatgtt gaggctgatg atgcgggtct caaggttggc
     3421 cttgacccgg ttctcatagc ccgcgtagga gtgcacaacg aaccactcgc ccggcttggc
     3481 gcgcagggtg gccttgaact cggcgaccgg gtcgatgacc tcagcggcct cttccgacga
     3541 ctctgcctca tccgacgatt ctgcgtcgtc ggcgtcagcc gtgacgtcag cgtcagcctc
     3601 gtcgacaacc aggtcggcgt cagcgccgtc gacctcgacc acctcaacgt catcgaggtg
     3661 cgtctcgtcc gcctcgatgt cgaggacttc gtcctggtcg gccacggcct ggacctactt
     3721 tccttgcgtc ggggggcgcc ggagcacccg aatgagacct gcccgcgaca gcgggaacgt
     3781 caccgtcaac tgccggcgaa gacccacagg acgaggcgct gcaggacgac gtcgagcccc
     3841 gccacgatgc ccatgatggc aacgacgaag aggatgacga ccacggtgta gttgagcagc
     3901 tcggaccccg tcgggcggac gaccttgcgc agttcgtcga ggatctgacg aatgaacagg
     3961 gtcagcgcag cgaagaaccg gctgaacggg ttaccgtcac cgggccgctg gcggacagca
     4021 cggctgcccg tccgcgcggt gctcgactcg ctcactcggc ctcttctcga tcaggtctta
     4081 ctacgtgacg cagggcaaga gggactcgaa cccccaaccg ccggttttgg agaccggtgc
     4141 tctaccaatt gagctattgc cctccaccgg acctggggag atccggaggc gatcgactgt
     4201 caagaaagcc ttgctgcggg cacgcctgag catggtctgg aaagccaacc gaagggaaag
     4261 catacgtgag gctgggcccc ggcgcgaacc ggcatacgcc gcgggcacac tccacgggat
     4321 tcgcccgcag tgcggaccag gctcccgaac aggcgcccag gagtgggagc atgacgcccg
     4381 tgaacgatct catcgtcccc cgccgtgttt cccagcgcat cggtgccatc gccgagagcg
     4441 ccaccctcgc cgtcgacgcc aaggccaaag ccctcaaggc ccaaggccgg ccggtcatcg
     4501 gcttcggtgc cggcgagccg gacttcccga cgcccgacta catcgtcgag gcggccatcg
     4561 cggcggcccg caaccccgcc aaccatcgct acacccccgc ggccgggctc cccgagctgc
     4621 gcgaggcgat cgcggccaag acgttgcgcg acagcgggta tgccgtgccc gccaccgccg
     4681 tgctcgtcac caacggcggc aagcaggccg tctaccaggc gttcgcctcg atcgtcgacc
     4741 cgggtgacga ggtgctgctg cccgcccctt actggacgac ctacccggag gccattgcgc
     4801 tggccggcgg ccgcgccgtc gaggtcttcg ccggtgagga ccagggctac ctggtcacgg
     4861 tcgagcagtt ggaggccgcc cgcaccgaga acaccaaggc gctgctgttc tgctcgccgt
     4921 ccaaccccac gggagcggtc tactcccccg agcaagtccg cgcgatcgga cggtgggcac
     4981 acgatcacgg catctgggtc atcaccgacg agatctacga acacctcacc tatgacggcg
     5041 tccgcgccgc gagcatcgtc gtcgaggtcc ccgagctcgc tgacacgtgc atcatcctca
     5101 acggagtcgc caagacctac gcgatgaccg ggtggcgggt cggctggatg atcggcccca
     5161 aggacgtcat cgctgcagcc accaacctgc agagccacct gacgagcaac
//
LOCUS       DAOMZY010000256         5193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_15017_length_5193_cov_5.570845, whole genome shotgun sequence.
ACCESSION   DAOMZY010000256 DAOMZY010000000
VERSION     DAOMZY010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15017_length_5193_cov_5.570845"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /gene="ychF"
                     /locus_tag="PLK64_13015"
     CDS             complement(<1..1073)
                     /gene="ychF"
                     /locus_tag="PLK64_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPV80714.1"
                     /translation="MALTIGIVGLPNVGKSTLFNALTKNNVLAANYPFATIEPNVGVV
                     PLPDPRLRRLAEIFGSEKILPATVSFVDIAGIVRGASVGEGLGNKFLANIREADAICQ
                     VVRVFVDDDVTHVEGKVSPSGDIETIETELVLADLQTMEKAVPRLEKEARIVKEKRAA
                     YENALAVQKCLEAGETLYAGHAAYGIDLAEARSLQLLTAKPFIYVFNLDEDQITDAAL
                     QASLRESVAPADCVFLSAKLEADLAELDEAEARELLESVGVTEPGLDQLAHVGFHTLG
                     LQTYLTAGPKESRAWTIRQGWTAPQAAGVIHTDFQRGFIKAEVVSFADLDAAGSMVAA
                     KAAGKVRIEGKEYVMSDGDVVEFR"
     gene            complement(1086..1934)
                     /locus_tag="PLK64_13020"
     CDS             complement(1086..1934)
                     /locus_tag="PLK64_13020"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPV80715.1"
                     /translation="MGTSDFEFDRATTLAAGAGAGEWVGQLDDQWRIGNGINGGLLMA
                     IGASALRADLDEDGAHPDPLAFTAYFQSPSEPGPVTVRTEVLRVGRSMSTGQVTLVQA
                     DAEGMPHERMRALATFGDLTDREEPRLRSAPPPTMPPPEQCIGGGDETPDFLAHSTLL
                     QRMDMRLDPASSGWLVGKPSHTGVLGGWLRLADGREPDVMSVLWALDALPPVAFDLGL
                     MGWTPTLEFSAHLRRHPAPGWLQVQLTTENVAGGLMEEDARIWDSEGNLVALSRQLCG
                     WRAPRG"
     gene            complement(1961..2581)
                     /locus_tag="PLK64_13025"
     CDS             complement(1961..2581)
                     /locus_tag="PLK64_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80716.1"
                     /translation="MSWLSPVELVGYLASALVVASLAMTNVVRLRTISLIGSVIFVVY
                     GALLGSIPIILTNAAVAGLNVWFLRKELGGGRTLGAVPIDAEAPFLQDFLRTHLPDIH
                     KSQPGFETVPADAYALLLTRDGLPAGALLGERRGSELRLTLDYVMEAYRDSRIGRWLY
                     TGDGQKVLRDAGFRTVVSTPATAVHRSYLQGVGFVPDGDALARILD"
     gene            2602..3867
                     /locus_tag="PLK64_13030"
     CDS             2602..3867
                     /locus_tag="PLK64_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HPV80717.1"
                     /translation="MLIQGAVGGRWEGQGMEFRELVIGALVGGLLGWLALRSWYAARL
                     ASAVTERDVLRERVLDLEATVSAEAETAALVAPLALSLDRVAQQVHTLERDRGEQFAR
                     VAAELARVQSSTGELRDQTASLVGSLASANVRGAWGEVTLRRVLEVSGLLARCDFDEQ
                     WTGVTMSGAAVRPDVVVHLPGERHLAVDAKAPMTDFLDAQADGLTAAERAKLLRAHAR
                     SLRGHVDALASKGYWTAFEGSPEIVVCFVPGEALLAAALSADPQLHEHAMARSVVLAS
                     PATLLALLRSVALAWRQDALADGARELLTLGQDLYARLGAVGRHTEALGASLRKSVEA
                     YNVFVGSMESRVLVTARRMHELGLASESVSVPSPEPVAVAPRPLTAAELLGALDADLE
                     RGGLTRPILVDHADSVERRGPGSIETAAG"
     gene            complement(3915..4970)
                     /locus_tag="PLK64_13035"
     CDS             complement(3915..4970)
                     /locus_tag="PLK64_13035"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPV80718.1"
                     /translation="MPPTPDPSSAAPSPSPTKRVLLAAPRGYCAGVDRAVVTVEKALE
                     LYGPPVYVRKEIVHNKHVVQTLRDRGAVFVDETDQVPEGATVIFSAHGVAPIVHEEAA
                     ARSLKTIDATCPLVTKVHREAVRFANEDFDILLVGHEGHEEVVGTSGEAPEHIVLVDG
                     PHAVAGVTVRDPEKVVWLSQTTLSVDETLETVAKLRERFPKLQNPPSDDICYATQNRQ
                     LAVKQMARDADLMIVVGSRNSSNSVRLVEVALEHGAGAGHLVDYAAEIDPAWLEGVTT
                     VGVTSGASVPEILVRDVLAFLAERGYADVQSVVAAEESLLFALPNELRRDLKARSGGD
                     PAPIRRDSSPQTPDPLH"
BASE COUNT          754 a         1777 c         1866 g          796 t
ORIGIN      
        1 cggaactcga ccacgtcgcc gtcagacatc acgtattcct tgccctcgat gcgcaccttg
       61 ccggccgcct tggccgccac catcgagccg gcggcatcca ggtcggcgaa cgacacgacc
      121 tcggccttga tgaacccgcg ctggaagtcg gtgtggatga cccccgcggc ctgcggcgcc
      181 gtccaaccct gccggatcgt ccaggcccgt gactccttgg gcccggcggt gagataggtc
      241 tgcagcccca gcgtgtggaa gccgacgtgg gccagttggt ccagccccgg ctcagtcacc
      301 ccgaccgact ccagtagctc ccgcgcctcg gcctcgtcga gctcggccag gtcggcttca
      361 agtttggccg agaggaacac gcagtccgcc ggtgcgaccg actcgcgcag ggacgcctgc
      421 aacgccgcat ccgtgatctg gtcctcgtcg aggttgaaca cgtagatgaa cggcttggcc
      481 gtcagcagtt gcagcgagcg cgcctcggcg aggtcgatcc cgtaggccgc gtgaccggca
      541 tacagggtct cgcctgcctc caggcacttc tggacagcga gcgcgttctc gtatgccgcc
      601 cgcttctcct tgacgatccg cgcttccttc tccaggcgcg ggaccgcctt ctccatggtc
      661 tgcaggtcgg cgagcacgag ctcggtctcg atggtctcga tgtcgcccga cggggagacc
      721 ttgccctcga cgtgcgtgac gtcgtcgtcg acgaagaccc gcacgacctg gcagatcgcg
      781 tcggcctcgc ggatgttggc gaggaacttg ttgcccagcc cttccccaac cgacgccccg
      841 cggacgatcc ccgcgatgtc gacgaacgac acggtggcgg gcaggatctt ctccgagccg
      901 aagatctcgg cgagccgccg cagccgcgga tcgggcaacg gaacgacgcc cacgttgggc
      961 tcgatcgtcg cgaaggggta gttcgccgcg aggacgttgt tcttggtcag cgcgttgaac
     1021 agggtggact tgccgacgtt gggcaggccg acgatgccga tggtgagtgc cacaagcggg
     1081 gaagtctacc cgcgaggggc gcgccacccg cacagctgcc gggagagggc gacgagattg
     1141 ccctcggagt cccagattcg ggcatcttcc tccatgagcc cgcccgcgac gttctccgtg
     1201 gtcagctgca cctgcagcca gcccggcgcg gggtggcgac gcaggtgcgc actgaactcc
     1261 agggtcggcg tccaccccat cagcccgagg tcgaaggcga ccggcggcag ggcgtccagc
     1321 gcccagagga ccgacatgac gtcgggctcg cgaccgtcgg ccaaccgcag ccagccgccc
     1381 aacacgcctg tgtgcgaagg cttcccgacg agccagccgg acgaggccgg gtccaggcgc
     1441 atgtccatcc gctgcaggag cgtcgagtgg gccaagaagt caggggtctc gtcgccgccg
     1501 ccgatgcact gttccggggg cggcatcgtc ggtggcggcg cgctgcgcag acggggttcc
     1561 tcccggtcgg tgaggtcccc gaaggtggcc agcgcgcgca tccgctcgtg cggcataccc
     1621 tccgcgtcgg cctgcacgag cgtgacctgc ccggtggaca tcgaccggcc cacccgcagc
     1681 acctccgtgc ggacggtcac cggaccgggc tcggacgggg actggaagta tgccgtgaac
     1741 gccaaaggat cggggtgggc gccatcctcg tcgaggtcgg cgcgcagggc ggaggccccg
     1801 atcgccatga gcagaccgcc gttaatgccg ttgccgatcc gccattggtc gtcgagttgc
     1861 ccgacccact cccccgcgcc ggccccagcc gccagggtgg tcgcgcggtc gaactcgaag
     1921 tcactcgtgc ccatcgcgtc agtctgtcat cgcgcgcgat ctagtcgagg atgcgagcga
     1981 gcgcgtcgcc gtccgggacg aagccgaccc cctgcaggta tgaccggtgg accgcggtcg
     2041 ccggggtcga caccaccgtg cggaatccgg cgtcccgcaa caccttctgc ccgtcgccgg
     2101 tatagagcca ccgcccgatc cgcgagtcgc ggtaggcctc catgacgtag tcgagggtca
     2161 gccgcaactc gctaccgcgc cgctccccga ggagggcgcc ggctggcagg ccgtcgcggg
     2221 tgagcaacag ggcataggcg tcggccggga cggtctcgaa acccggctga ctcttgtgga
     2281 tgtcgggcag gtgggtgcgc aggaagtcct gcaggaacgg cgcctccgcg tcgatgggga
     2341 cggcgccgag ggtgcgcccg ccgccgagtt ccttgcgcag gaaccacacg ttgagccctg
     2401 ccacggccgc gttggtgagg atgatcggga tcgacccgag cagcgcccca tagacgacga
     2461 agatcaccga tccgatgagc gagatggtgc gcagacgcac gacgttcgtc atggcgagcg
     2521 acgcgacgac aagggccgac gcgaggtagc cgaccaactc gacggggctc agccaggaca
     2581 cgtcggcgag catacgggcg cgtgctcatc cagggggctg tcggtgggcg atgggagggt
     2641 cagggcatgg agtttcgtga gttggtgatc ggggcgctcg tcggtggcct tctcgggtgg
     2701 ctggcactgc ggtcgtggta tgccgcgcgc ctcgcgtccg ctgtcaccga gcgtgacgtg
     2761 ctgcgcgagc gggtgctcga cctggaggcg acggtgtcgg ccgaggccga gacggctgcc
     2821 ctggtggccc cactggcgtt gagcctcgat cgggtggccc agcaggtgca cacactggag
     2881 cgcgatcggg gcgagcagtt cgcccgggtg gccgccgagt tggcccgcgt gcagtcgagc
     2941 acgggcgagt tgcgtgacca gaccgcgtcc ctggtggggt cgctggcgtc cgccaacgtg
     3001 cgcggggcct ggggcgaggt cacgctgcgc cgggtgttgg aggtgtcggg gctgttggct
     3061 cgctgcgact tcgacgagca gtggacgggg gtgacgatgt ccggcgcggc ggtgcggccc
     3121 gatgtcgtgg tgcacctgcc cggcgagcgg cacctcgccg tcgacgccaa ggcaccgatg
     3181 accgacttcc tggatgccca ggctgatggg ctgactgcgg ccgagcgggc caagctcttg
     3241 cgggcccacg ccaggtcgct gcgcggtcac gtcgacgcgt tggcgtccaa gggctactgg
     3301 acggcgttcg agggctctcc ggagatcgtc gtctgcttcg ttccgggcga ggcgctgctg
     3361 gctgcggcct tgtcggccga ccctcagttg cacgagcacg cgatggcccg ctcggtcgtg
     3421 ctcgcctcgc cagcgaccct gctggccttg ttgcgctcgg tggcgttggc ctggcgacag
     3481 gatgctctcg ccgacggagc ccgcgagttg ctcacgctcg gccaggacct ctatgcccgc
     3541 ttgggtgcgg tggggcggca taccgaggcg ttgggcgcga gcctgcgcaa gtcggtggag
     3601 gcctacaacg tcttcgtcgg gtcgatggag agccgggtgc tggtcacggc tcggcggatg
     3661 catgagttgg ggctagccag cgagtcggtc agcgtgccct cccccgagcc ggtcgccgtg
     3721 gcaccccggc cgctgacggc ggccgagctc ttaggcgcgc tggacgccga tctggagcgc
     3781 ggtgggctca cccgtccgat attggtcgac cacgccgatt ccgtggagcg ccgtggcccc
     3841 gggtcgatcg agactgcagc cggctgacca ccgccgtccg gacaacggac gcagaaaggc
     3901 tcagtgccga cgggtcagtg cagggggtcg ggcgtctgcg ggctggaatc gcgccggatc
     3961 ggtgctgggt cgccgccgga gcgggccttg aggtcgcggc gcaactcgtt gggcagggcg
     4021 aagagcagcg actcctcggc cgcgacgact gactgcacat cggcataccc gcgctcggcg
     4081 aggaaggcca acacgtcgcg cacgaggatc tccggcacgc tcgcccctga ggtcaccccg
     4141 acagtcgtga cgccctccag ccaggccggg tcgatctcag cggcgtagtc gaccagatgc
     4201 ccagccccgg caccgtgctc cagcgcgacc tcgacgaggc ggaccgagtt cgaggagttg
     4261 cgtgagccca cgacgatcat caggtcggcg tcgcgagcca tctgcttgac ggccaactga
     4321 cggttctggg tggcataaca gatgtcgtcg ctcggcgggt tctgcagctt ggggaagcgc
     4381 tcgcgcagct tggccaccgt ctccagcgtc tcgtcgaccg acaacgtggt ctgcgagagc
     4441 cacacgacct tctcggggtc gcggaccgtc accccggcga cggcatgcgg accgtcgacg
     4501 agcacgatgt gctccggggc ctcccccgac gtgccgacga cctcctcgtg cccctcgtgc
     4561 ccgacgagca ggatgtcgaa gtcttcgttg gcgaagcgca cggcctcgcg gtgcaccttg
     4621 gtgaccaagg ggcaggtcgc gtcgatggtc ttgagcgacc gggcagccgc ctcctcgtgg
     4681 acgatcgggg ccactccgtg cgccgagaag atcaccgtgg cgccctcagg cacctggtcg
     4741 gtctcgtcaa cgaacacggc gccccggtcc cgcagggtct gcacgacgtg cttgttgtgg
     4801 acgatttcct tgcgcacata gaccggcggc ccatacagct ccagcgcctt ctcgaccgtg
     4861 acgacagcgc ggtcgacgcc cgcgcaatac ccgcgcggcg ccgccagcag gacccgcttg
     4921 gtggggctcg gcgagggcgc agcagaggag gggtcggggg tgggcggcat accgtcaatg
     4981 ctacgtgcgc atcgagggct cccgtccctg ccgcgggcca ggcgcttccc cccgcaccaa
     5041 ccgttgccgc gtcgaccacc acgacaggcc gcgcgctcgt cgggaaggcg gaatacagtc
     5101 gcggttgtga gtatgccgcc cgcccccgac ccgcgatcct cgtcgcgtgc gtcgggctcg
     5161 cgggtgcccg cgagcgcgcc cggcgcccgg gcg
//
LOCUS       DAOMZY010000257         5159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_15142_length_5159_cov_5.209639, whole genome shotgun sequence.
ACCESSION   DAOMZY010000257 DAOMZY010000000
VERSION     DAOMZY010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15142_length_5159_cov_5.209639"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PLK64_13040"
     CDS             complement(<1..506)
                     /locus_tag="PLK64_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HPV80719.1"
                     /translation="MPSGPLLLAFLATAAVIVAIPGPSLLFTIGRAITVGRRAALWTV
                     VGNAAGLLVQSFLVAVGLGAVLAASSAAYTVVKLAGAAYLVYLGVQAIRHRGELAASL
                     LAARGGEAAGVGSGVAASAAVDRRSLFQGLTVGLTNPKTLVFLSSLLPQFVDPASAPQ
                     PQILALGAV"
     gene            complement(532..2520)
                     /locus_tag="PLK64_13045"
     CDS             complement(532..2520)
                     /locus_tag="PLK64_13045"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPV80720.1"
                     /translation="MTAETEALSERLGPYRLIQRIGEGGMGVVHLALDPHGRAVAVKV
                     LRPHVAHDPEARARLEREVATLARIRHPRVAPIIDCDLSGDRPFVVTRYIAGDALDDR
                     VDTRGPLSAGDLLLLARGLHGALEAIHAVGVVHRDLKPGNVLLEDDGEPVVIDFGIAH
                     VAEDPRLTATGLVMGTPGYLSPEVIEGAAVTAATDWWGWAATLAFAATGRPPFGRGSM
                     NIVLTRVRAGNSDLEGVDARLEPLLSAALSPRPDDRPPAEIVMAALERYAAGGFATDV
                     MPIHGGGLGVGGVGAAGSGGPGRSVAPRHTAVLPTQPPTGAPYPVPPPGPAPAAGYAA
                     PAAGYGGPAVGGYAAPAPGYAGPAPAGYAAPAPAPGYADPAPAGYAAPAPAPGYAGPT
                     PGGYAAAPVPWPSEPPWHAPASLAGPGAALGPAARVVPSSLAGEAGARGGAEDFDGDE
                     RGAYLPGSGAADPRIGRPDRTGTLAVGGLFVVALAAALPVVALLVVLAWSLLARTADR
                     SMTSIVLRRFHYGSRRGDVPVTVALGPWHLLTGSLAAAVGLLLPLLVGIAGVFCVALA
                     LSATTASSPNPNHPIALGGGMALALLMAWWGPGGASLRRGSRSVMRGVVREGLVTSVV
                     SALFVVGGLGALVWLVLHGGSPSWAPMSGPPGFFSSWR"
     gene            complement(2644..3072)
                     /locus_tag="PLK64_13050"
     CDS             complement(2644..3072)
                     /locus_tag="PLK64_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPV80721.1"
                     /translation="MKISDVLRNKGTEVVTIRPDETVSRLLEALAEHRVGALVVSPDG
                     AAVAGIVSERDVVRHLHQRGAAVLVIPVSEIMTADVTTCTADDSVESLAHTMTDRRIR
                     HVPVVAEGALVAIVSIGDIVKHRIDSLQSERDQLVEYIQQ"
     gene            complement(3133..4221)
                     /locus_tag="PLK64_13055"
     CDS             complement(3133..4221)
                     /locus_tag="PLK64_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPV80722.1"
                     /translation="MVRARQRDIAQAAGVSEATVSRVLNDHPGVSEATRQSVMVALDV
                     LGFERPTYLRRKSAGLVGLILPELTNPIFPAFAQLISTALARRGFIPILCTQTAGGVH
                     EDDYVQQLLDHGVAGMINISGLHADSDSKPGRYFRLRELGMPLVLVNGAIDGLDVPTI
                     ANDDVAAIEMAIAHLTELGHVRIGLAAGPDRFIPVIRKLWGFRGAMARHTSLSRAEID
                     DLIVHELFTVDGGAAAAARLLDRGVTAIAAASDLMALGAVRAVRARGLRVPQDVSVIG
                     YDDSPLIAFSDPPLTTIRQNVTAMSETAVNSLVEIIGGSDSRAKEFTFRPELVVRGST
                     APCVVQARERHTPRARGLRAAKVSDERP"
     gene            complement(4225..>5159)
                     /locus_tag="PLK64_13060"
     CDS             complement(4225..>5159)
                     /locus_tag="PLK64_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPV80723.1"
                     /translation="DGGLDGWRVDVANMTGRHRGADHYHQVARDLRSASVAAKPDALV
                     IAEHCHDVSTDLTGDGWHGVMNYAGFTRPVWTWLTSPTNPDAPADFLGQPVRVPRLPG
                     ELVADAMIDFTSRIPWATLVRSFTLLGSHDTSRIRTLVGGDLALVRAAAGLLFTMPGT
                     PMVTYGDEIGMRGRFGEDGRRPMPWPAGDGTHGETWDEGLLAAYRDLAGLRRGSVALL
                     RGGLRWVYAAGDALVFLREAPGETALVHVARAAHDPVRIDVRDLLGISDSMVGFGGGG
                     ELEAFTLTLGATGPDVSVRIWATGTGGSNGWGGR"
BASE COUNT          884 a         1932 c         1730 g          613 t
ORIGIN      
        1 accgcaccga gagcgaggat ctgcggctgc ggcgcactcg ccgggtcgac gaactgcggc
       61 agcagcgacg acaggaacac cagcgttttg gggttggtca gcccaaccgt cagcccctgg
      121 aaaagactcc gccgatccac cgccgcggac gccgccacac cggatccaac cccagccgcc
      181 tcgcccccgc gcgcggccaa caacgacgcc gccaactcgc ctcggtgccg gatcgcctgc
      241 acccccagat agaccaggta tgccgccccc gccagcttca ccaccgtgta ggccgccgac
      301 gacgcagcca gcaccgcacc cagtccgacc gccaccaaaa agctctgcac gagcagcccg
      361 gccgcattgc cgaccaccgt ccacaaggcc gcccggcgcc ccaccgtgat ggcccgcccg
      421 atggtgaaca gcaggctcgg cccaggaatc gcaacgatca ccgccgccgt cgcgaggaag
      481 gcgagcagga gcggaccgct gggcatggcc gccagtatgc cgcccgcttc ctcagcgcca
      541 ggacgagaag aacccgggtg gtccagacat cggcgcccac gagggcgacc caccgtgcag
      601 caccagccac acgagcgcgc cgagcccgcc gacgacgaac aacgcgctga cgacactcgt
      661 caccaggccc tcgcgcacga cgccacgcat caccgaccgg gacccccggc gcagcgacgc
      721 cccgccgggc ccccaccacg ccatgagcag ggccagcgcc atgccgccgc cgagcgcgat
      781 cgggtggttc gggttggggg agctcgccgt tgtcgccgag agcgcgagcg ccacgcagaa
      841 caccccagcg atgccgacga gcagcgggag gaggaggccg acggccgcgg ccaacgatcc
      901 ggtcaggagg tgccatggcc ccagggcaac cgtgaccggc acgtcgccgc gccgcgagcc
      961 gtaatggaat cggcgcagca cgatcgatgt catcgaccga tcggcggtgc gggcgagcaa
     1021 ggaccacgcc aggacaacga gcagcgcgac gaccggcagc gcggccgcaa gcgcgacgac
     1081 gaacaggccg cccaccgcca gggttccggt gcggtcaggt cgaccgatgc gcgggtcggc
     1141 agcaccagag ccgggcaggt aggccccccg ctcgtcaccg tcgaagtcct ccgcgccccc
     1201 tcgtgcgccg gcctcgccgg ccagcgacga aggcaccaca cgagcggccg gccccaacgc
     1261 cgccccaggc cccgccaagg acgcgggtgc gtgccacggg ggctcgctcg gccacgggac
     1321 gggcgccgcg gcataccccc caggagtggg cccggcataa ccgggggctg gcgcgggcgc
     1381 ggcatacccc gcgggcgccg ggtcggcata accgggggct ggcgcgggcg cggcataccc
     1441 cgcgggcgcc ggtccggcat aaccaggggc aggcgcggca taccccccaa cagccggtcc
     1501 gccatacccc gcggcaggcg cggcataccc cgcggcagga gccggtccgg gcggaggcac
     1561 ggggtaaggc gcgcccgtcg gcggctgggt cggcaagacg gcggtatgcc gtggcgccac
     1621 cgagcgaccc ggcccaccag acccagccgc cccaacaccc ccaacaccca aaccaccacc
     1681 gtggatcggc atgacgtcgg tagcgaaccc accggcggca taacgttcga gcgcagccat
     1741 aacgatctct gccggtgggc gatcgtcggg gcgcggcgac aacgccgccg acagcagcgg
     1801 ctcgagccgc gcgtcgaccc cctcaaggtc actgttgccg gcgcggacgc gcgtgaggac
     1861 gatgttcatc gagccccgac cgaacggcgg gcgcccggtc gccgcgaagg ccaacgtcgc
     1921 ggcccacccc caccagtcgg tggcagcggt gacagcagcc ccctcgatga cctcgggcga
     1981 cagatacccc ggcgtcccca tgaccagccc cgtcgcggtc aaccgagggt cctcagccac
     2041 atgggcgatg ccaaagtcga tgacgacggg ctccccgtcg tcctccagca gcacattgcc
     2101 cggcttgaga tcccggtgca ccaccccgac ggcgtggatc gcctccaggg cgccatgcaa
     2161 cccacgcgcc aggagcagca aatcgccggc cgacagcggc ccacgagtgt cgacccggtc
     2221 atcgagggcg tcaccggcga tataccgcgt gacgacgaaa ggccggtccc ccgacaggtc
     2281 gcagtcgatg atcggcgcga ccctcggatg ccggatccgg gccagggtcg cgacctcacg
     2341 ctcgagccgc gcgcgtgcct ccggatcgtg tgcgacgtgc ggccgcaaca ccttgacggc
     2401 gacggcgcgc ccatgcgggt cgagagccag atggacaacg cccatccccc cttcgccgat
     2461 ccgttggatc aggcggtagg ggcccagtcg ctcagagagc gcctcggtct cggccgtcat
     2521 gggacaacgg taggtgaggg cacccctacc gttggcatgc tcggcgtgtc gcctcaccga
     2581 gatcgggtgg ccccaacgtc cccaccggga gtcccggcca cacgatccgc gtatgccgcc
     2641 cgcttactgc tggatgtact ccacgagttg gtcacgctcg gactgcagcg agtcgatgcg
     2701 gtgcttgacg atgtcgccga tgctgacgat ggccaccagg gcaccctcgg cgacgaccgg
     2761 cacgtggcgg atccgacgat cagtcatcgt gtgggccaac gactccaccg agtcgtccgc
     2821 ggtgcacgtg gtcacgtcgg cggtcatgat ctcgctcacc gggatgacca ggaccgcggc
     2881 accccgctgg tgcagatggc gaacgacgtc acgctcgctg acgatcccgg caacggccgc
     2941 accgtccggg ctgacgacga gcgcgccgac tcgatgctcg gcgagggctt cgagcaggcg
     3001 ggagacggtc tcatcaggcc ggatcgtgac gacctcggtc cctttgttac gcagcacgtc
     3061 ggagatcttc atacccggac cgtagacccc cgcgacgact caggccagcg ttagcatcac
     3121 gcgaattccc gttcagggcc gttcgtcgct caccttcgca gcgcgcagcc cccgggcccg
     3181 aggggtatgc cgttcgcgcg cctgcacgac gcacggggcc gtcgagccac gcaccaccaa
     3241 ctcgggccgg aaggtgaact ccttggcccg ggagtccgac cccccaatga tctcgaccaa
     3301 ggagttgacg gccgtttcgc tcatcgccgt gacgttctgg cggatcgtcg tcagcggcgg
     3361 atcgctgaac gcgatgagtg gtgagtcgtc gtagccgatc accgagacgt cctgcggcac
     3421 ccgcagccca cgggctcgca cagcgcggac cgctcccaac gccatgaggt ccgaggccgc
     3481 ggcgatagcc gtgacgccac ggtccaacaa ccgcgccgca gccgccgctc ccccgtcgac
     3541 cgtgaagagt tcgtggacga tgaggtcgtc gatctcggcc cggctcaacg aggtatgccg
     3601 cgccatcgcc cctcggaagc cccacaactt gcggatcacc gggatgaacc gatcggggcc
     3661 ggccgccaac ccgatccgca cgtgccccag ctcggtcagg tgggcgatcg ccatctcgat
     3721 ggcggcgaca tcatcgttgg cgatggtggg gacatcgagc ccgtcgatcg ccccgttgac
     3781 caggacgagc ggcataccca actcgcgcag tcggaagtag cggccaggtt tggagtcgga
     3841 gtcggcgtgc aggccggaga tgttgatcat cccggcgacc ccatggtcga gcaactgctg
     3901 gacgtagtcg tcctcgtgga cgccaccggc cgtctgcgtg cagaggatcg ggatgaagcc
     3961 gcgacgggcc aaggctgtcg agatgagctg cgcgaaggcc gggaagatcg ggttggtgag
     4021 ctccggcagg atgagcccca cgaggccagc actcttgcgc cgcaggtagg tcggtcgctc
     4081 gaagccgagc acgtccagcg cgaccatgac cgactgtcgg gtggcctcgg acacgccggg
     4141 atgatcgttg agcacccggc tcaccgttgc ctcggacacc cccgcagcct gggcaatatc
     4201 ccgttgccgc gcgcgcacca tcgcctacct ccctccccag ccgttcgacc ccccggtccc
     4261 cgtcgcccag atccggaccg acacgtcagg cccggtggcg ccaagggtga gcgtaaacgc
     4321 ttccaactcc cccccgccac cgaatcccac catgctgtcc gagatcccca gcaggtcccg
     4381 cacgtcgatc cgcaccgggt catgcgccgc gcgcgcgacg tgcacgagcg ccgtctcccc
     4441 cggcgcctcg cgcaggaaga cgagggcgtc gccggcggca tacacccagc gcaggccgcc
     4501 ccggagcaag gcgaccgacc cgcggcgcag gccggcgaga tcccggtatg ccgcgagcag
     4561 cccttcgtcc cacgtctcac cgtgggtgcc gtccccggcc ggccacggca tcgggcggcg
     4621 gccgtcctca ccgaagcgcc cgcgcatgcc gatctcgtcg ccataggtga ccatcggtgt
     4681 gccgggcatg gtgaacaaca gccccgccgc ggcccgaacc agcgccagat cgccgcccac
     4741 aagagtgcgg atgcgtgacg tgtcgtgcga gccgaggagc gtgaaggacc gcacgagggt
     4801 ggcccacggg atccgcgagg tgaagtcgat catcgcgtcg gcaaccagct cacccggaag
     4861 gcgcggcacc cgcaccggtt gaccaaggaa gtcggcgggg gcgtcggggt tggtcggcga
     4921 cgtgagccag gtccacaccg ggcgggtgaa cccggcgtag ttcatcaccc cgtgccaacc
     4981 gtcaccggtc aggtcggtgg acacgtcgtg gcaatgctcg gcgatgacga gcgcgtcggg
     5041 cttggccgcc acactcgccg agcgcaggtc gcgggcgacc tggtgatagt ggtcggcgcc
     5101 gcgatggcgc ccggtcatgt tggcgacgtc gactcgccag ccgtccagcc cgccgtcgg
//
LOCUS       DAOMZY010000258         5086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_15441_length_5086_cov_6.158815, whole genome shotgun sequence.
ACCESSION   DAOMZY010000258 DAOMZY010000000
VERSION     DAOMZY010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15441_length_5086_cov_6.158815"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PLK64_13065"
     CDS             complement(<1..587)
                     /locus_tag="PLK64_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80724.1"
                     /translation="MTVDDHPDRPAARSAGRPRRPLYLDSAGLEAQAGSFDPVQEAQA
                     AHESAAILVHRGRASDDPELTARLVALAEEVGLDTLAHLWATRPARSLPGALWRLYVL
                     RDWVRRDPAGASREYAAGIRYAAVNHVVAGPADPPGPEELRTLLDAVLRGVFEGDLAV
                     ALERAAAFARVVSAGRAAESEGEAAATQASDLLATA"
     gene            766..840
                     /locus_tag="PLK64_13070"
     tRNA            766..840
                     /locus_tag="PLK64_13070"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:798..800,aa:Lys,seq:ttt)
     gene            970..1653
                     /locus_tag="PLK64_13075"
     CDS             970..1653
                     /locus_tag="PLK64_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80725.1"
                     /translation="MTTEHGSGRHRPPGAGGAVGMRSMSVIGAFGIVAMVLLLVGWGL
                     WSVFGGQRPSGQPQAQAASSIGAIGASAEPQGTDPAVNATSSATDPTGLMDPTATATS
                     TAVPTSTAALTSPLGGGPSSTTTGTRTSTTSSTTTPGPAPTTTTPAPQPWGVTNVQFT
                     CTLTGRRVQAVLTFTSTENVTAILRAGESTTTKTGSGGVSVTVSGLPTPADCWAAVDG
                     RTVGTIPAT"
     gene            1744..2370
                     /locus_tag="PLK64_13080"
     CDS             1744..2370
                     /locus_tag="PLK64_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80726.1"
                     /translation="MGRHRDEAADGLDSSRRTTVIIGAIGVLAVALVFGAWWMLSSSP
                     SIGKGATALSTARVTLNEVPGATDAAGSTATFVVPTPSFVTPSLAVALGSREPEIPGE
                     TATTTTPAGTSSTTSSSSSSSSSSNGRVTVSNLSLQCGLQGRRVRATLTFHSTGQVPV
                     TLTASDRTENTVASGNVRLDVIGAAPASGTATCSAKVNGMSVGPIAAH"
     gene            2410..3462
                     /locus_tag="PLK64_13085"
     CDS             2410..3462
                     /locus_tag="PLK64_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPV80727.1"
                     /translation="MLGLAPLAAGCSPVDALRRIAMLLERSRAGTYRVQAFRTAATVV
                     AGMPPTELSSRLAAGTLGELPGLGPATVGVVTQAADGELPAYLASLQEQAAVSLAPGG
                     EGLYAALTGDLHSHSDWSDGGSPIQEMVLSAVELGQEWLALTDHSPRLKVANGLSAER
                     LRRQLGVVAGVNAALGQRFRLLSGIEVDIVDDGSLDQEPGLLAELDVVTASVHSKLRM
                     PRDPMTRRMLAAVANPRVNVLGHATGRLVEGSRGTRPQSEFDAEAVFAACVEHDVAVE
                     INSRPERCDPPDDLVVIARDLGCLFAIDSDAHAPGQLDMKAYGCERATRLGIPAERIV
                     TTWPVDQLLEWAARGR"
     gene            complement(3472..4695)
                     /locus_tag="PLK64_13090"
     CDS             complement(3472..4695)
                     /locus_tag="PLK64_13090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HPV80728.1"
                     /translation="MTRRQVPRWSELRDLVKLQRPELSPTRRHLGAALTVADLRDVAR
                     RRVPRAVFDYVDGAAETEASYRRARAAYEGIEFQPRVLQDVGALSTATTVLGAPAAVP
                     FCFAPTGFTRLMHHEGEAAVARVAGERGIPYAVSTLGTSTPEAVADEVRGLPGSRLWF
                     QLYVWRDRAAAEELMARADAAGYEALVLTVDVPVSGARLRDVRNGLSVPPALTTKTVL
                     DMARHPAWWGNVLTTAPIDFAALAGWDGTIGDKLNALFDPTMTLADLDWVRRFWRKPL
                     IVKGIQTLDDARAVTDAGADAIVLSSHGGRQLDRAPVPLRLVPAVRETLGPRAQILVD
                     SGIMSGADVVAAIALGATSTLVGRAYLFGLMAGGQAGVERVTDILTGEVRRTMALLGA
                     ATVADLNPGHVRLAP"
     gene            4921..4993
                     /locus_tag="PLK64_13095"
     tRNA            4921..4993
                     /locus_tag="PLK64_13095"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4955..4957,aa:Glu,seq:ttc)
BASE COUNT          704 a         1809 c         1823 g          750 t
ORIGIN      
        1 gccgtcgcca gcaggtcgga cgcttgggtg gccgcagcct ccccctccga ctccgcggcc
       61 cggcccgcac tgaccacccg cgcgaacgcc gcggcccgct ccaacgccac cgcgagatcc
      121 ccctcgaaaa cgccccgcag aacggcgtcg agcagggtgc gcagttcttc tggcccaggc
      181 ggatccgccg gccccgcgac gacgtggttg acggcggcat accggatgcc cgcggcatac
      241 tcacgactcg ctccggcggg gtcgcggcgc acccagtcac gtagcacgta gagccgccac
      301 agcgcgcccg ggaggctgcg cgcgggccgc gtcgcccaca ggtgcgcgag ggtgtccagc
      361 ccgacctcct cggcgagcgc cacgaggcgt gcggtgagct ccgggtcgtc cgatgcgcgc
      421 ccccgatgca cgaggatcgc ggcactctcg tgcgccgcct gcgcctcctg gacggggtcg
      481 aacgagccgg cctgcgcctc gaggcccgcg ctgtccaggt agagcggtcg ccggggtcgg
      541 ccagcgctgc gggcggcggg gcggtccggg tgatcgtcga cggtcatggc gttccttcct
      601 ggtcgcggcg gcgggtggca gcggacgggg gctgcgcggc ataccccttg gggtggggcg
      661 ggttgccgac ccctcacggt atgccgcccg cgcgcggtcc gcgggtaacg gggccggtcc
      721 gttaggctgg ctgatgcttc gggccggcgg gtgtcggcct ctgccgggtc tctagctcaa
      781 tggcagagct gcggactttt aatccgtagg ttgtgggttc gagccccacg ggacccacca
      841 tgctccctcg gccgaggcgc tgctccggcg ccgcgccgaa ccctcctgga cacccctggc
      901 cctctggcgc ctgcggattt cccacttcgc catggttcgg ctgccggtcg cgccatatcc
      961 tggccagcaa tgacgacgga gcacggatcc ggtcgccatc ggcctcccgg tgccggtggc
     1021 gccgtcggta tgcgctcgat gtcggtcatc ggcgccttcg ggatcgtcgc gatggttctc
     1081 ctgctcgtcg ggtggggcct gtggtcggtg ttcggcggcc aacgaccgtc gggccagcct
     1141 caggctcagg ccgcgtcgtc gatcggagcg atcggggcga gcgccgagcc gcagggcacc
     1201 gatccggcag tgaacgccac ctcttcggcc acggacccga cgggcctcat ggacccgacg
     1261 gccacagcga cctcgacggc cgtgccgacc tcgacggcag ccctcacgtc accgctcggc
     1321 ggcggaccgt cgagcacgac aaccggaacg cgcacgtcga ccacctcgag cacgaccacg
     1381 ccggggccgg cgccgacgac gaccactccc gcccctcaac cgtggggggt gacgaacgtg
     1441 cagttcacct gcaccttgac ggggcgtcgg gtgcaggcgg tcctcacctt cacctcgacc
     1501 gagaacgtca cggccatcct gcgggccggg gagtcgacga cgaccaagac cgggtccggg
     1561 ggcgtctccg tcaccgtgtc ggggctgccc acgcccgccg attgttgggc cgccgtcgac
     1621 gggcgtactg tcggaacgat ccccgccacc tgaccgtgtg acgttcaacc atttctccac
     1681 gggtggtgtg tcggctaatg tgacacgctg gaacggtctc ggggacacag accgggagac
     1741 tgaatgggca ggcaccgcga cgaagcggcc gacggcttgg acagtagtcg gcgcacaacc
     1801 gtcatcatcg gcgccatcgg cgtgctcgcc gtggcgctcg tcttcggagc atggtggatg
     1861 ctgtcgtcgt ccccctccat cggcaagggc gcgacggcgc tgtcgaccgc gcgagtgacc
     1921 ctcaacgaag tgcctggggc gaccgacgca gccggatcga ccgcgacgtt cgtggtcccg
     1981 acgccgagtt tcgtgacgcc gtcgctcgcg gttgcgttgg gtagccgaga gcccgagatt
     2041 ccgggcgaga ccgcgacgac aacgacccct gcagggacat cgtcgaccac ctcgtcgtcc
     2101 tcgtcctcgt catcgtcctc caacggtcgc gtgacggtct ccaacctgtc cctgcagtgc
     2161 ggactccaag gccgccgggt ccgggccacg ctgaccttcc actcgaccgg gcaggttccc
     2221 gtgacgctca ccgccagcga tcggaccgag aacaccgtgg cgtccggcaa cgtccgcctc
     2281 gatgtcatcg gtgccgcccc cgcctctggc actgcgacgt gctcggccaa ggtcaacggc
     2341 atgtcggtcg ggccgatcgc ggcccactga cgggccgggc gctcgccgcc gcgccgatag
     2401 ggtgacccaa tgctcgggct tgcccccctc gccgctggat gttctcccgt cgatgcgttg
     2461 cgccggatcg cgatgttgct ggagcgctcg cgggcaggca cttatcgcgt ccaagccttc
     2521 cgcaccgcgg caacagttgt cgcgggtatg ccgcccaccg agctctcatc gcgcctcgcc
     2581 gccgggacgc tcggggagct tccgggcctc ggaccggcca ccgtcggagt ggtgacgcag
     2641 gccgcggacg gtgagctgcc ggcatacctc gcgtccttgc aggagcaggc ggcggtctcg
     2701 ctcgccccgg gaggcgaggg cctgtatgcc gcgctgaccg gcgatctgca cagccactcg
     2761 gactggagcg acggggggtc cccgatccag gagatggtgt tgtcggccgt cgagttgggg
     2821 caggaatggc tggcgctgac cgatcactca ccgcgactga aggtggccaa cgggttgagc
     2881 gcggagcggc tgcgtcggca gctcggggtc gttgctggcg tgaatgccgc tctgggccaa
     2941 cggtttcggc tgctcagcgg cattgaggtc gacatcgtcg atgacgggtc gctcgaccag
     3001 gagccggggt tgttggcgga gctggacgtc gtcacggcat cggttcactc caagctgcgg
     3061 atgccgcgcg acccgatgac ccggaggatg ctggcggcgg tcgccaaccc gcgggtcaac
     3121 gtcctcgggc acgcgaccgg tcgactggtg gagggctcgc gggggacgcg gccgcagtcg
     3181 gagttcgacg ccgaggcggt gtttgcggcg tgcgtcgagc acgacgtcgc ggtcgagatc
     3241 aactcgcggc ctgagcgctg cgaccccccg gatgatcttg tggtgatcgc gcgcgacctg
     3301 gggtgtttgt tcgcgatcga ctcggatgcg cacgcgccgg ggcagttgga catgaaggcc
     3361 tacggctgcg agcgcgcgac gcggttggga atccccgccg agcggatcgt caccacctgg
     3421 ccggtcgacc agctgttgga gtgggccgcc cgcggccggt gagcgcggcg gtcagggcgc
     3481 gagtcggacg tggccggggt tgaggtctgc gaccgtggcc gcgccgagga gcgccatcgt
     3541 gcgccgcacc tcccccgtca ggatgtcggt cacccgctcg acgccagcct gcccgccggc
     3601 catgaggccg aagaggtagg cgcgcccgac cagcgtgctc gtcgcgccga gggcgatcgc
     3661 ggccacgaca tcggcgcccg acatgatgcc ggagtcaacg aggatctgcg cccgcggccc
     3721 cagcgtctcc cggaccgccg gcaccagccg caacggcacg ggcgcgcgat cgagttggcg
     3781 gccaccgtgg ctggacagga cgatggcgtc cgcccccgcg tcggtcaccg cgcgagcatc
     3841 gtcgagggtc tggatcccct taacgatcaa cggttttcgc cagaacctcc gcacccagtc
     3901 gaggtcggcg agggtcatcg tcgggtcgaa gagcgcgttg agtttgtcgc cgatcgtgcc
     3961 gtcccacccc gcgagagccg cgaagtcgat cggcgccgtc gtgagcacat tgccccacca
     4021 ggccgggtgc cgggccatat cgagcaccgt cttggtggtg agcgccggcg gcaccgacaa
     4081 gccgttgcgg acatcgcgca gccgcgctcc cgacaccggc acgtccacgg tgaggaccag
     4141 cgcctcgtag cccgccgcat ccgcccgagc catcaactcc tcggccgccg cccggtcgcg
     4201 ccacacatag agctggaacc acaacctcga ccccggcaag ccgcgcacct cgtccgcgac
     4261 cgcctcgggg gtggacgtgc cgagcgttga cacggcatac gggatgccgc gctcccccgc
     4321 cacgcgcgcg accgccgcct cgccctcgtg gtgcatgagc cgcgtgaagc cggtcggcgc
     4381 gaagcagaac ggcaccgcgg ccggggcgcc gaggacggtg gtcgccgtcg acagcgcgcc
     4441 gacgtcctgc aagacgcggg gctggaactc gatcccctcg tatgccgccc gcgcccgccg
     4501 gtagctcgcc tcggtctccg cggcgccgtc gacatagtcg aagacggccc gcgggacccg
     4561 gcggcgggcc acgtcgcgca gatcggccac ggtgagcgcg gcgccgaggt gccgacgggt
     4621 cggggagagc tcaggtcgct ggagtttgac gaggtcacgc agctcggacc aacgcggcac
     4681 ctgacgacga gtcatggccc cactctcccc gtcgggcggg agcggcgcca gagtggtccg
     4741 gggtgcgcgt tcgggggtgg gaggcggcgc agccgggggt gagcggcata ccctcaggac
     4801 gggttggggc gccacgaacc tgcgatttgg agggccccgg cgcggtcccc tatgcttacc
     4861 gggtcctcaa cggatccgag catggctctg aggcagcgag gatctgaggt cacatcccag
     4921 gtccccatcg tctagcggcc taggactccg ccctttcacg gcggcagcac gggttcgaat
     4981 cccgttgggg atacgttgtg caagcagttc ggtcttgggc attagcccgc tcaaggcccc
     5041 gtagcgcagt tggttagcgc gccgccctgt cacggcggag gtcgcg
//
LOCUS       DAOMZY010000259         5048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_15597_length_5048_cov_5.299619, whole genome shotgun sequence.
ACCESSION   DAOMZY010000259 DAOMZY010000000
VERSION     DAOMZY010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15597_length_5048_cov_5.299619"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="PLK64_13100"
     CDS             complement(<1..969)
                     /locus_tag="PLK64_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HPV80729.1"
                     /translation="MPGQSRFADLEPLLDRVQKPIQYVGGELNSQVKDWQCGGHGPGG
                     EELTVRWALMYPDAYEVGLPNQGVMILYEVLNERPHVLAERTYAVWPDLEALLRESGV
                     PQLTVDGHRAVVDFDLFGVSFSTELGYTNFLTAIDLAGIPLHSKDRGDGTPIVIAGGH
                     ASFNPEPIADFVDAVVVGDGEQAVLEITDLVAAWKAQGSPGGRDELLMRIARSGVAYV
                     PSCYEVDYFPDGRIRRVAPRTGLTGIPWRVSKHTVTDLDAWPYPKAPLVPLAETVHER
                     MSVEIFRGCTRGCRFCQAGMITRPVRERSITGIGEMVERGLAATGFE"
     gene            complement(1074..2930)
                     /locus_tag="PLK64_13105"
     CDS             complement(1074..2930)
                     /locus_tag="PLK64_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPV80730.1"
                     /translation="MKSAVDERVNHDILEGRARSIAALFRDRVSRSGNREAFQYFHNG
                     HLQSVTWSAARDRVYDMAAGLMSLGLTIEDRVAIASTTRYEWALADLGVMCSGGATTT
                     VYPTTMAEDVAYILGDSGSRFVFAEDDGQVEKLRTHRHDMPNVVKVIVFNGEAHSGDE
                     EGDDWVISLADLEALGRDAREARPTMVDERIDLIAPDHLAVIIYTSGTTGRPKGAELT
                     HDAIVYEAAAVHAIGILDQNDLQFLWLPLSHVFGKILLALPLQMGFPTAIDGKIDKIV
                     DNLAVIKPTFMGAAPRIFEKAYARIAATVAAETGVKRQLMDWALDVGKQVMELRAQGQ
                     APGALLGIQHAVADKLVLSKIRERFGGRVRFFISGSAALNPEIARWFGSVGLIILEGY
                     GLTETSAATCVNRPSLGGYNYGTVGWAIPTTDVKLAADGEILVKGPGVMRGYRNRPDA
                     TAEVFDEDGYFRTGDIGEIDANGFVKITDRKKDLFKTSGGKYIAPSLIEAAFKAQCPY
                     ASQILVFGDGRNYATALITLDPDAITAWGAANRMAGKSYAEISQTPEVRELLQGYIDT
                     LNQGLNRWETIKRFTVLDRDLTIDEGELTPSLKLKRKHVAEKFKANLEALYD"
     gene            complement(3074..3580)
                     /locus_tag="PLK64_13110"
     CDS             complement(3074..3580)
                     /locus_tag="PLK64_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HPV80731.1"
                     /translation="MAGPSLPVLRLPTWQELLGRPESLRRMMNVWPPYRFAGIRVLHI
                     EPDYSRALVSLKLTPWNRNYVGTQFGGSLSSMADPFWMLLVMNQLGRDYIVWDQRAEI
                     EFVRPGTGEVRTEFVVDPAVVERIRQAAAGGQKVLEWFENDIVDMSGVLVARVRRQLY
                     VRRKRDTV"
     gene            complement(3645..4601)
                     /locus_tag="PLK64_13115"
     CDS             complement(3645..4601)
                     /locus_tag="PLK64_13115"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HPV80732.1"
                     /translation="MQVWNGLDEVPSGIGPCVVTLGNFDGVHRGHRAVLGVVVDAATS
                     RGLTSVAVTFHPHPIAVLFPDRAPAELTSLAQRLDLLSATGLDAVLVLEFTREFAGQT
                     PAEFVDSVFVDALNAQLVVVGADTRFGVRNSGTVQTLIELGADRGFEVAPIADVGDEG
                     RWSSTRVRALVADGDVVAAGELLGRSPRVDGVVVHGDHRGRQLGFPTANLGDPIEGLV
                     PADGVYAGWLTRLGRPLGASDRVLPAAISIGTNPTFDGTSRRVEAYVLDRTDLDLYGE
                     RVAYEFVRMLRPTLRFDGIEPLVVQMHADVQECRETLGVPAP"
     gene            complement(4612..>5048)
                     /gene="truB"
                     /locus_tag="PLK64_13120"
     CDS             complement(4612..>5048)
                     /gene="truB"
                     /locus_tag="PLK64_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147622.1"
                     /note="catalyzes isomerization of specific uridines in RNA
                     to pseudouridine; responsible for residues in T loops of
                     many tRNAs; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPV80733.1"
                     /translation="GTTVDVDIEVDVSSGTFVRALARDLGTALGVGGHLVALRRTRVG
                     CFTIGEAHDLEALSTRSGEGGTGAHGIPLLSLAAAGRRALPVRDLSEAEARALGFGQP
                     VASSGPAEEAVAAFTPDGHLLAVLDQTKPRARTRVVFPIEDS"
BASE COUNT          835 a         1690 c         1734 g          789 t
ORIGIN      
        1 ctcgaagccg gtcgctgcga gcccccgctc caccatctcg ccgatgccgg tgatcgaccg
       61 ctcgcgcacc gggcgggtga tcatccccgc ctggcagaac cggcagcctc gggtgcaccc
      121 ccggaagatc tccaccgaca tccgctcgtg gaccgtctcg gcgagcggga cgagcggggc
      181 cttcgggtag ggccacgcgt ccaggtcagt gaccgtgtgt ttggagaccc gccacggtat
      241 gccggtcagc ccggtccgcg gcgcgacccg gcggatccgt ccgtcgggga agtagtccac
      301 ctcgtagcag gacgggacgt aggcgacacc cgagcgcgcg atccgcatga ggagttcgtc
      361 gcggcctccc gggctcccct gggccttcca ggcggccacg aggtcggtga tctccagcac
      421 cgcctgctcc ccgtcaccga cgacgaccgc atcgacgaag tcggcgatcg gctcggggtt
      481 gaacgacgcg tggcccccgg cgatgacgat tggagtgccg tcgccgcgat ccttggagtg
      541 cagcggtatg ccggctaggt cgatggcggt gaggaagttg gtgtagccca actcggtgga
      601 gaacgacacc ccgaacaggt cgaagtcgac cacggcgcga tggccgtcga cggtcaactg
      661 gggcacgccg ctctcccgca gcagcgcctc gaggtccggc cacacggcat acgtgcgctc
      721 ggccagcacg tggggacgct cgttgaggac ttcgtagagg atcatgacgc cctggttggg
      781 cagcccgacc tcgtaggcgt cggggtacat cagcgcccac cggaccgtga gttcctcgcc
      841 gccggggccg tggccaccgc actgccagtc cttgacctgc gagttcaact cgccgccgac
      901 atactggatc ggcttctgga cgcggtcgag cagcggctcc aggtcggcga agcgggactg
      961 tccaggcatc cgtcgatcct accggcgtca cgtgcagagg ggcacgaaca gcggctgggc
     1021 cggaatctgt tcgtacccct cgtcacgggt gatgcgcccg tcgggcggac ctgtcagtcg
     1081 tagagagctt ccaggttggc cttgaacttc tcggcgacgt gcttgcgctt gagcttgagg
     1141 ctgggggtca gctcgccctc gtcgatcgtc aggtcgcggt ccaggaccgt gaaccgcttg
     1201 atcgtctccc agcggttgag gccctggttg agggtgtcga tgtagccctg gagcaactcg
     1261 cgcacctcgg gggtctgact gatctcggca taggacttgc cggccatccg gttggctgcg
     1321 ccccacgcgg tgatggcgtc ggggtcgagg gtgatcagcg ccgtggcgta gttgcgacca
     1381 tcgccgaaga cgaggatctg cgaggcgtag gggcactgcg ccttgaaggc cgcctcgatc
     1441 aacgagggag cgatgtactt gccacccgag gtcttgaaca ggtccttctt gcggtcggtg
     1501 atcttgacga agccgttggc gtcgatctcg ccgatgtcac cggtgcggaa gtagccgtcc
     1561 tcgtcgaaga cctctgccgt ggcatcggga cggttgcggt agccccgcat gacgcccgga
     1621 cccttgacga ggatctcgcc gtcggcggcc agtttcacgt cggtcgtcgg gatcgcccag
     1681 ccgaccgtgc cgtagttgta cccaccgagg gacgggcggt tgacacaagt ggctgccgag
     1741 gtctcggtca ggccgtaacc ctcgaggatg atcaggccca ccgacccgaa ccagcgggcg
     1801 atctccgggt tgagcgctgc cgaacccgag atgaagaagc ggacccgccc accgaagcgc
     1861 tcacggatct tggagaggac gagcttgtcg gccacggcat gttggatacc gagcagcgct
     1921 ccgggcgcct ggccttgcgc acgcaactcc atcacctgct tgccgacgtc gagagcccaa
     1981 tccatgagct gacgcttgac gccggtctcg gccgccaccg tggcggcaat ccgggcgtag
     2041 gccttctcga agatgcgagg ggcggcaccc atgaaggtgg gcttgatgac ggccaggttg
     2101 tcgacgatct tgtcgatctt gccgtcgatc gcggtcggga agcccatctg caacggcagg
     2161 gcgagcagga tcttgccgaa cacgtgggac agcggcagcc agaggaactg caagtcgttc
     2221 tggtcgagga tcccgatggc gtggacggcc gccgcttcat agacaatcgc gtcgtgggtc
     2281 agctcggcgc ccttggggcg cccggtcgtt cccgaggtgt agatgatgac ggccaggtgg
     2341 tcgggcgcga tgagatcgat ccgctcgtcg accatcgtcg ggcgggcctc acgggcatcg
     2401 cggcccagcg cctcgaggtc ggccaacgag atcacccagt cgtcgccctc ttcgtcgcca
     2461 ctgtgcgcct caccgttgaa gacgatgacc ttgacgacgt tgggcatgtc gtgacggtgc
     2521 gtccgcagct tctcgacctg gccgtcgtcc tcggcgaaca cgaaccgcga cccggagtca
     2581 cccaggatgt aggcgacatc ctcggccatc gttgtggggt agaccgtcgt cgtcgcaccg
     2641 ccagaacaca tgacgccgag gtcggccagg gcccactcgt agcgcgtggt cgaggcgatc
     2701 gccacccggt cctcgatggt gaggccgagc gacatcaggc cggcagccat gtcgtaaacg
     2761 cggtcgcggg cggcgctcca cgtgacgctc tggagatggc cgttatggaa gtactggaat
     2821 gcttcgcggt tccccgagcg ggacacccgg tcacggaaga gagcggcgat gctgcgggct
     2881 cgtccttcca ggatgtcgtg gttgacgcgc tcgtccacgg ctgacttcat cgccacgggc
     2941 tttgactcct ttgtcgttac tctcgtcgtc ggcggacggt gcctactagt cagtagatcg
     3001 ccgttgctcg accgctcgtg ctacccccaa ccgtatgctg tcccggctcc aggcgggagc
     3061 cccagggtct aactcacacc gtgtcgcgct tgcgccggac atacagttgg cggcgcaccc
     3121 tggccacgag gacccccgac atgtcgacaa tgtcgttctc gaaccattcg agcacctttt
     3181 ggccgccggc cgcggcctgt cggatccgtt ccaccacagc gggatccacc acgaactcgg
     3241 tgcggacctc gcccgtgccc gggcggacga actcgatctc ggcccgctgg tcccagacga
     3301 tgtagtcccg gcccaactgg ttcatcacca gcagcatcca gaacggatcg gccatcgagg
     3361 acaacgaccc gccgaactgg gtccccacgt agttgcggtt ccagggcgtc aacttcaggc
     3421 tgacgagggc ccgcgagtag tcgggctcga tgtgcaggac ccggatgccg gcgaaccgat
     3481 agggcggcca gacgttcatc atgcgccgca ggctctcggg gcgaccgagc aactcctgcc
     3541 aggtgggcag ccgaaggacg ggtaaggaag gaccagccat ccccccagcc tgccatgggc
     3601 cggctgccgg cacctcagcg ggcggaaccc cagcgggcgg cacctcaggg ggctggaacg
     3661 cccaacgtct cgcgacactc ctggacatcg gcgtgcatct gcacgaccag cggctcgatg
     3721 ccgtcgaacc gcagcgtggg gcgcagcatc cggacgaact cgtaggcgac ccgctcgccg
     3781 taaaggtcga ggtcagtccg gtccaggacg tacgcctcga cccggcggga ggtcccgtcg
     3841 aaggtcgggt tcgtgccgat cgagattgcc gcgggcagca cccggtcgga ggcaccgagg
     3901 gggcggccca ggcgggtgag ccacccggca tacaccccgt ctgctgggac gaggccctcg
     3961 atggggtcgc cgagattggc tgtcgggaag ccgagttggc gcccgcggtg gtccccgtgg
     4021 acgacaaccc cgtcgacccg cggggagcgg ccaagcagct cgcctgctgc gacgacgtcg
     4081 ccgtcggcga ccagagcccg gacgcgggtc gaggaccagc ggccctcgtc accgacatcg
     4141 gcgatcggag cgacctcgaa acctcggtcg gcacccaact cgatgagggt ctggacggtt
     4201 ccgctgttgc gcaccccgaa gcgggtgtcg gctccgacga cgacgagttg cgcgttcaag
     4261 gcgtcgacga acaccgagtc gacgaactct gccggcgtct ggccagcgaa ttcgcgggtg
     4321 aactccagca cgagcaccgc gtccagcccc gtggcggaga gcaggtccag ccgttgggcg
     4381 agcgaggtga gctcggcggg tgcgcgatcg gggaagagca ccgcgatcgg atgcgggtgg
     4441 aaggtgaccg ccaccgaggt caggcctcgg gaggtcgcag cgtcgacgac aacgccaagg
     4501 accgcgcgat ggccccggtg cacgccgtcg aaattgccga gggtgacaac gcacgggccg
     4561 atgcccgaag gcacctcgtc gagaccgttc cagacctgca cggaggacac tctaggagtc
     4621 ctcgatgggg aacacgacgc gcgtccgcgc ccgaggcttg gtctgatcaa ggacggcaag
     4681 caggtgaccg tcgggagtga acgccgcaac agcctcctcc gcgggcccgc tcgaggccac
     4741 cggttggccg aaaccgaggg ctcgcgcctc cgcctcggac aggtcccgaa ccggcagggc
     4801 gcggcggcca gcggcggcca aggacagcag aggtatgccg tgcgccccag tcccgccctc
     4861 ccctgacctg gtcgacaacg cctccaggtc gtgagcctca ccaatggtga agcaccccac
     4921 ccgggtgcga cgcagggcca cgaggtgccc gccgactccg agcgccgttc ccaggtctct
     4981 ggcgagggct cggacaaagg tgccggatga cacgtccacc tcgatgtcca cgtcgaccgt
     5041 cgtcccgg
//
LOCUS       DAOMZY010000260         5044 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_15620_length_5044_cov_6.778112, whole genome shotgun sequence.
ACCESSION   DAOMZY010000260 DAOMZY010000000
VERSION     DAOMZY010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15620_length_5044_cov_6.778112"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..365)
                     /gene="ssrA"
                     /locus_tag="PLK64_13125"
     tmRNA           complement(1..365)
                     /gene="ssrA"
                     /locus_tag="PLK64_13125"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(471..2003)
                     /gene="mptB"
                     /locus_tag="PLK64_13130"
     CDS             complement(471..2003)
                     /gene="mptB"
                     /locus_tag="PLK64_13130"
                     /inference="COORDINATES: protein motif:HMM:NF038066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol phosphomannose-dependent alpha 1,6
                     mannosyltransferase MptB"
                     /protein_id="HPV80734.1"
                     /translation="MPSSPGHDRAHVAPAGWTGRIRREFDDLRYAWGDPVTRWGALGS
                     LLILIGAYGPSTMPNSNFWRDLPVIGLMQTTLPGRILATGSMLIGVVILLDAWLRLRP
                     SKGHRPLTRGTVWWWSLPLLLSPPLLSFDAFSYAAQGELVSRGFDPYKVGPGVLGDAF
                     TSQVDQMWRDTPAPYGPLSLQLQHLIVVVTGHQPYLSALLVRIPAFIGVLLLAVYLPR
                     LARRIGYDRETTIWLAVLNPLVLLHLVGGAHNDALMIGLMMWGLWVAAKTHWVPAAVI
                     IAAAASVKQPAAAAVLAVAALHAWRVGGWREQPSNATVARYAVGVVGVFLAAFVAIGL
                     LTGLGFGWITALGVPNAVRSFLSPVTTIAQGLDWVLVSLGVIPDADLVVPIAQRVGQV
                     VGLLVVIWMVFKFKARHPVRTLGWILLVLVFTGPVVHPWYILWGGLLFAMTESGQLAR
                     RIVVWATCGLAMYSVVDAAWRSSVVAVGVTAALALVWITTGHDRDLIRDKDFAKSRRT
                     PA"
     gene            complement(2046..3299)
                     /locus_tag="PLK64_13135"
     CDS             complement(2046..3299)
                     /locus_tag="PLK64_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV80735.1"
                     /translation="MTSTLSTRAAVQRRTIATLLSTQVLGGLGVGAAISVNAMLAKDV
                     SGVESLAGLAQTAAVLGTALVTLLIARVMDDHGRRIGLTLGYALGFIGTALSIVAGVT
                     RVFPLLLVGAVFLGATTAANNQSRYAATDLSLPAHRGRDLSLVVWATTVGSVLGPNLS
                     GPGKVVASAVGLPPLTGAYLFSGVGMLAAMAVMFFRLRPDPLVLARAEHLKELPAGTA
                     PTARASGRALLRENRLLRLAAIGMSLSHAVMVSVMVMTPIHMDHGHASLEIIGLVISI
                     HILGMFVFSPAVGMAVDRWGSAPILAAGGVVLLVALVLAGSSHAGASWTLGLGLFLLG
                     VGWSLGTIACSTLVTAASTPHTRPAIQGVTDLLTSLAGALGGAIAGLVVGLAGYSALN
                     IGAAVLAVGVVTTAVAVGRQTESAD"
     gene            3389..>5044
                     /locus_tag="PLK64_13140"
     CDS             3389..>5044
                     /locus_tag="PLK64_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HPV80736.1"
                     /translation="MFGKVLVANRGEIAVRAFRAATELGAKTVAVFPHEDRSSEHRLK
                     ADEAYEIGEPGHPVRAYLNPRAIVEVAVACGAQAIYPGYGFLSENPDLAEACESSGVV
                     FVGPPAAVLHLTGNKSRAIEAARRAGLPTLRGCPPGTDVEALVAGAEEIGFPVFVKAV
                     AGGGGRGMRRVDAPADLRGALAAAMREAESAFGDPTVFIEEAVIRPRHIEVQILADAV
                     GNVVHLFERDCSVQRRHQKVVEVAPAPNLDPALRDQMCADAVAFAREIGYVNAGTVEF
                     LLAENGRYVFIEMNPRIQVEHTVTEEVTDIDLVASQLRIASGETLPDLGISQDTITLR
                     GAALQCRITTEDPANEFRPDTGEITAYRSAGGSGVRLDGGTVFVGAQVSPHFDSMLVK
                     LTCRGRDFATAVTRARRALAEFRIRGVSTNIPFLQGVLEEPDFVAGRATTSFIDERPH
                     LRTARTPHDRGTKLLTYLADVTVNQPHGPAATRISPRAKLPTLDRYAAPPAGARDRLL
                     ELGPERFAAALRAQVPVAVTDTTFRDAHQRLVGIAGGRVGDRHG"
BASE COUNT          850 a         1745 c         1774 g          675 t
ORIGIN      
        1 gtggaggtgg cgggaatcga acccgcgtcc gatgacgtga tcccaggact tctccgggcg
       61 cagtgcgcta tggagtttct cggccccagg gctcgcgcgc acgcgttccc tgacaggccc
      121 agtcgagtag atgtcccacg ccgccccccg acatgacggc gcggtgagtt tcctagctga
      181 cgccaggcac tgagtcggaa actatctcag gctgacggac ttcgaggctc gctcaggcgg
      241 cgagggcgaa gtcggtgcgc tttgagttgg cacctattgg tttgcgcgga tcgttaacga
      301 gataaccgtg cgtcctcggc ccgcttctcc tgtgacgacg atcaccgtcg aaaccgatca
      361 cccccgtgag gggtgtccac gtcacgctgt gcagttgtca agaaccttca gtatgccgtg
      421 caacggctac cggggtcgac tccattcccg ggcggggcca gatcgcaagc tcaggccggg
      481 gtccgacgcg acttggcgaa gtccttgtcg cggatgaggt cccggtcatg gccggtggtg
      541 atccacacca gggccagcgc tgcggtgacc ccgacggcga cgactgaact gcgccaggca
      601 gcgtcgacga cggagtacat cgccagacca caggtcgccc agacgacgat ccgtcgggcc
      661 agttggccgg actcggtcat ggcgaagagc aggccgcccc agaggatgta ccaagggtgg
      721 acgacggggc cggtgaagac caggacgagc aagatccacc cgagggtgcg gaccgggtgc
      781 cgcgccttga acttgaacac catccagatg acgacgagca ggccgacgac ctgaccgacc
      841 cgctgcgcga tcgggacgac gaggtcggcg tcggggatga ccccgaggct gacgaggacc
      901 cagtccagtc cttgagcgat ggtcgtgacg ggcgagagga agctgcgcac ggcattgggc
      961 acgcccaggg cggtgatcca gccgaagccg agcccggtca gcagcccaat ggccacgaag
     1021 gccgcgagga acacgccgac gacgccgacg gcataccggg cgactgtggc gttgctcggt
     1081 tgctcgcgcc agcccccgac ccgccaagcg tgcaaggcgg cgacggcgag cacggctgcg
     1141 gccgcgggct gtttcaccga cgctgccgcc gcaatgatca ccgctgcggg cacccagtgc
     1201 gtcttagcgg ccacccagag cccccacatc atgaggccga tcatgagagc gtcgttgtgg
     1261 gctccgccga cgaggtgcag caggaccaag gggttgagca cggccaacca gatcgtcgtc
     1321 tctcggtcgt agccgattcg gcgggccagc ctcggcagat agaccgcgag caacagcacc
     1381 ccgatgaacg cggggatccg cacgagcagc gccgacaggt agggctggtg gccggtgacg
     1441 acgacgatga ggtgctgcag ctgcagcgac agcgggccgt agggggccgg ggtgtcccgc
     1501 cacatctggt cgacctggct ggtgaaggcg tcgcccagca cccccgggcc gaccttgtag
     1561 gggtcgaagc cacgcgagac gagctcgccc tgggcagcat acgagaatgc gtcgaaggac
     1621 agcagcggtg gcgagaggag cagcggcagc gaccaccacc acaccgtgcc gcgggtcagc
     1681 gggcggtgtc ccttggacgg ccgcagccgc aaccacgcgt ccagcaggat gacgaccccg
     1741 atgagcatgg agccagtcgc caggatccgg ccggggagcg tcgtctgcat gaggccgatg
     1801 acgggcaggt cccgccagaa gttcgagttc ggcatcgtcg acgggccgta ggcaccgatg
     1861 aggatgagca ggctgcccag cgccccccac cgggtgaccg ggtcgcccca ggcgtaacgg
     1921 agatcgtcga actcccgccg gattcgcccc gtccagccgg cgggcgcgac gtgggcgcgg
     1981 tcgtgacccg gggaggaggg catcgcgctc cactcgtcgt ggcggtcgtg gggatcgtcc
     2041 ctaccttagt ccgcagattc ggtctgccgc cccacggcga ccgccgtcgt gacgacgccc
     2101 acggcaagga ccgcggcgcc gatgttgagg gccgagtatc ccgccagccc cacgacgagc
     2161 ccagcgatcg ccccgcccaa cgcgcccgcc aggctggtga gcaggtcggt gacgccctgg
     2221 atcgcggggc gcgtgtgcgg cgtcgacgcc gcggtgacca gggtcgagca ggcgatcgtg
     2281 ccgagggacc agccgacccc gaggaggaac aagccgagcc cgagcgtcca ggaggcaccg
     2341 gcgtgggacg agccggccag cacgagcgcc acgaggagca cgactccgcc cgcggcgagg
     2401 atcggggcgc tcccccaccg gtcaacggcc atgccgacgg cgggcgagaa gacgaacatc
     2461 ccgaggatgt ggatgctgat gaccaggccg atgatctcca acgaggcgtg gccgtgatcc
     2521 atgtggatcg gagtcatcac catgaccgag accatgaccg cgtggctcag gctcatgccg
     2581 atcgccgcga gacgcagcag tcggttctcc cgcagcagag ctcgtccgga ggcccgggcc
     2641 gtgggcgccg tcccggcagg gagttccttg aggtgttcgg ccctggccag cacgagcggg
     2701 tcggggcgca accggaagaa catgaccgcc atcgcggcga gcatgccgac gccggagaac
     2761 aagtaggccc ccgtcagtgg gggcagtccg acggccgaag cgacgacctt gcccggcccg
     2821 gacaggttgg ggcccaggac cgagcccacc gtcgtggccc acaccacgag cgagaggtcc
     2881 cgcccgcggt gggccgggag cgaaaggtcg gtcgcggcat accggctctg gttgttggcc
     2941 gccgtggtcg cgccgaggaa gaccgccccg acgagaagca gcgggaacac ccgggtgacg
     3001 ccggccacga tggacagcgc cgtgccgatg aaccccagcg catagccgag cgtcagcccg
     3061 atgcgtcggc cgtggtcgtc catcacccgc gcgatcagga gcgtgaccag agcggtcccg
     3121 aggacggccg cggtctgggc caaacccgcc aacgactcga caccggacac atccttggcc
     3181 agcatggcgt tgacggagat cgcagcaccg accccgaggc caccgagcac ttgggtggac
     3241 agcaaggtcg cgatggtccg tcgctgcaca gccgcacgcg tcgagagggt cgaggtcact
     3301 cgcaagagtc tgacaggcga accgcacggc ggggcttcgc ggctgatggc tacgctgccg
     3361 caactggcac gaggaatcgg aggctgacat gttcggcaag gtcttggtgg ccaaccgcgg
     3421 agagatcgcg gtccgcgcgt tccgggcagc gaccgagttg ggcgcgaaga cggtcgcggt
     3481 gttcccccat gaggaccggt ccagcgagca tcggctcaag gccgacgagg cctacgagat
     3541 cggtgagccg ggccatcccg tccgtgccta cctgaatccg cgggccatcg tcgaggtggc
     3601 cgtcgcgtgc ggcgcccagg cgatctatcc cggatacggc ttcctgtccg agaaccccga
     3661 cctcgccgag gcgtgcgaga gctccggcgt ggtcttcgtc ggtccgcctg ctgcggtcct
     3721 gcacctcacc ggcaacaagt cgcgggccat cgaggcggct cggcgcgccg gcctgccgac
     3781 gctgcgcggt tgcccgcccg gcaccgatgt cgaggccctc gtcgcgggcg ccgaggagat
     3841 cgggttcccc gtgttcgtca aggcggtcgc cggtggcggc ggccgcggca tgcgtcgggt
     3901 cgacgctccc gcagacctgc gcggcgcgct ggccgcggcg atgcgcgaag ccgagtcggc
     3961 cttcggcgac ccgacggtct tcatcgagga ggcggtgatc cgcccccgcc acatcgaggt
     4021 gcagatcctc gccgatgcgg tcggcaacgt cgtgcacctc ttcgagcgcg actgttcggt
     4081 ccaacgccgc caccagaagg tcgtcgaggt cgctcctgcg cccaacctcg acccggcgtt
     4141 gcgcgaccag atgtgtgccg acgccgtcgc gttcgcccgc gagatcggtt acgtcaacgc
     4201 cggcacggtg gagttcctcc tcgccgagaa cggccgctac gtcttcatcg agatgaaccc
     4261 gcgcatccag gtcgagcaca cggtcaccga ggaagtaacc gacatcgacc tggtggcctc
     4321 gcaactgcgg atcgcctcgg gcgagaccct cccggacctc gggatcagcc aggacacgat
     4381 caccctgcgc ggggccgccc tgcagtgccg cattacgacc gaggacccgg cgaacgagtt
     4441 ccgccccgac accggcgaga tcacggcata ccgcagcgcg ggtggctccg gcgtgcgact
     4501 ggacggtggc acggtcttcg tcggcgctca ggtgagcccg cacttcgact cgatgctcgt
     4561 caagctcacc tgccgcgggc gcgatttcgc gaccgcggtg acccgcgcgc gccgggcgtt
     4621 ggccgagttc cgcatccgtg gcgtttccac caatatcccc ttcctgcaag gggttctcga
     4681 ggagcccgac ttcgtcgccg gccgcgcgac cacctcgttc atcgacgagc ggccgcacct
     4741 gcgcaccgct cggacgccgc acgaccgcgg cacgaagttg ctcacctacc tcgccgatgt
     4801 gacggtcaac caaccgcacg gccccgccgc gacccgcatc tcaccgcgcg cgaaactccc
     4861 cacgctcgat cggtatgccg ccccgcccgc cggtgcgcgc gaccgactgc tggagttggg
     4921 ccccgagcgg ttcgccgccg cgttgcgggc ccaggtgccc gtggcggtca ccgacacgac
     4981 cttccgcgac gcccaccagc gactggtggg catcgcggga ggtcgggtcg gtgaccgcca
     5041 cggg
//
LOCUS       DAOMZY010000261         5043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_15630_length_5043_cov_3.983761, whole genome shotgun sequence.
ACCESSION   DAOMZY010000261 DAOMZY010000000
VERSION     DAOMZY010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15630_length_5043_cov_3.983761"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            328..537
                     /locus_tag="PLK64_13145"
     CDS             328..537
                     /locus_tag="PLK64_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80737.1"
                     /translation="MTSRADQGKQPFWGPGQPLVVDQWSSQFGAAEVPVHRNLQDLAR
                     RAKSCKIPRTTTRNLDRISTPVEMH"
     gene            complement(653..3205)
                     /locus_tag="PLK64_13150"
     CDS             complement(653..3205)
                     /locus_tag="PLK64_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80738.1"
                     /translation="MTVYSDITSWAATRPWWQQQALVTLASTGDDAAFDYEALVHSML
                     STPPATPPGGWLAGIQQPLEQPASAVSLVSIHGVDNVNRLVAGQTLTFAPSGVTVIYG
                     NNGSGKSGYARLVKSLVRTRDAAEVLPDIFRSTSGSVTAALSFFEGGTVATVRLGDEA
                     PPSLQQITFYDERCGDLYVSKEGEASYRPSALALFDGLIRVCDTVRAAIDSRLADLSR
                     NRVALPELDAATPAAQFLSSLNADTTDDEIASACTVSRDAEAEIERARSEEARLRATD
                     PTTERTRIEELSGSYLRAAQLLAEARTSLDAVAERQLRTLQSEAAEARQVARAMAAER
                     TISDPLDGVGSALWRTLWLAAERYSATEVYPEHEFPNTDEGAACPLCQQHLDSAARDR
                     LTRFQRLMTDTSESIARRLEERLDTEMSEWLAAGRDGDSLALTLRDLDEPLAAAAAAQ
                     YRVWGERRDAVEVGRDALPDEPNDELEQSLRAKSAQYAADAILIDTDTFNQRVHGLVT
                     AQRELAAAIALAGAKRLIMGERDRVRQETALREARRDTDTRGISRTLGDLTAKHVTVV
                     VQDRFSRESQDLQVDSVTLRGQGVRHGSVLHKPEFVGAHLEADLPRVLSEGEQTALGL
                     AGYFVEAHLDASMSSLVLDDPVTSLDHLRRERVARRLIEFAKSRQVIVFTHDAVFAGD
                     LRRAASEEGVEYTARSVERRGLENAPGYCHDDHPWKAKDAASRLHSLREDLARMRRES
                     TGWDDDTRERAIGSWSGRLSETWERIVSQEIAGQLFDRCTEEVRPTMLKVLVKFNEED
                     DKAFQEAYKRVSRWATRHDKSTALNYVPPSFDELSKELDIAKAWFERVRKFK"
     gene            complement(3337..>5043)
                     /locus_tag="PLK64_13155"
     CDS             complement(3337..>5043)
                     /locus_tag="PLK64_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HPV80739.1"
                     /translation="LGDIRFIDPACGCGNFIIVAYRELREIELQVMEALYDLRDKHQL
                     SLDAKSDLKVTLDHFYGIEIDEWPVRIAETAMFMMDRQCDLKLQERFGQAPERLPIQR
                     EAKIVVGNALRTQWEMLLPPTQNVVVAGNPPFLGISGRSAEQTADLQWVWGSGYHGSL
                     DYVTGWYARALKYFGEAPGRFAYVSTNSITQGEPVGPLFRPILGADWRIRFAHRTFPW
                     TSDASGKAAVHCTIIGFDRGGPPARLFDYPSSKAGPTELPTNGSITPYLTFGPPVIVD
                     PSGHPLNPQVGKVAYGNKPTDGGWLIVEGDERTGVESDPIARKYLRPYVGARELLHGG
                     DRYCLWLVGAPATDIQGSPVLRERVEGVRTFRLDSDAASTRQAASTAHLFRQIAQPDT
                     SYLCIPAHVSEERPYFLAGHFGPEVIASNANFVAPDPDGLVFALISSSMFVAWQRAVG
                     GRIKSDLRFNKLLTWNTFPVPTLQSAQRSAIAGAGEAVLLARSRHSEGSLAAMYAPGA
                     MGEVLASAHAALDHEVNAAFNIRDDATELQRQSHLFGLYATATSGLLAGLASKGRVGA
                     RR"
BASE COUNT          916 a         1576 c         1604 g          947 t
ORIGIN      
        1 ctgccgagaa cctctaacaa gatgaacgcc agaaacaccg ttcatcggac agtcccgcac
       61 ctgggatgcg aaaacacccg agcagcagca ggcaaccctc gatcggctct acgaagcgaa
      121 acgccccaac ttcttcccat gcggaaccgt cgcgtcgtac ggacgaggtt gccgatgcga
      181 cgactgccgg accgcgcaca ccgagtatcg acgagcccgg cgcaacgccg cggcgcaacg
      241 cgaccgcgac gagggaggga gcccccaccg cgactcgtca cagctcccca gcgctcccca
      301 gcctgcgacc catccgcccg agaagctatg acgagtcgcg ccgaccaggg gaaacagccg
      361 ttctggggcc ctggccagcc gctggtcgtc gaccagtggt cctcgcagtt cggggcggct
      421 gaggtgccgg tgcaccgcaa cttgcaggac cttgcgcgac gggccaagtc gtgcaagata
      481 ccccgcacga cgacgcgcaa tctcgaccgc atctcgacac ctgtcgagat gcactgaatc
      541 ttgtaagaac ggcccttgat tctaagaatt agccgacgtt atgcacctca ggcgtgtccg
      601 atagttggtg ttatgtgttg accccgccct cgacaggtgc ccttggtagt ggctacttga
      661 acttcctaac gcgttcgaac caagccttgg caatgtccag ttccttcgac agttcgtcaa
      721 agctcggtgg gacgtagttc aacgcggtgg acttgtcgtg acgggtggcc caccttgaca
      781 cccgtttgta ggcctcttgg aacgctttgt cgtcttcctc gttgaacttg acgagcacct
      841 tgagcatcgt gggacggacc tcttcggtgc atctgtcgaa cagttgtccc gcgatctcct
      901 ggctgacgat gcgttcccac gtctcggaca gcctccctga ccaggaacct atcgctcgct
      961 cgcgggtgtc gtcgtcccat cctgtcgact cgcgccgcat tcgagccaga tcctcccgca
     1021 gtgagtgaag ccgagacgcc gcatccttgg ccttccatgg gtggtcgtcg tggcagtagc
     1081 ccggagcatt ttccaggcct cgacgttcga cggagcgcgc tgtgtactcg acaccctcct
     1141 cgctggctgc acgtcggagg tcccccgcga atacggcgtc gtgggtgaac acgatgactt
     1201 gccggctctt cgcgaactcg atcaggcgtc gggccactcg ctcgcgacgt aggtggtcga
     1261 gactggttac tgggtcatcg aggaccaaag atgacataga ggcgtcgaga tgagcctcca
     1321 cgaagtagcc ggcaagccca agtgctgttt gttcgccctc ggatagaacg cgcggaaggt
     1381 cggcctcaag gtgagcaccc acgaactcgg gtttgtgcaa cacggatccg tgcctcacac
     1441 cttgtcctcg gagtgtcacc gaatcgacct gcaagtcttg ggactcccgt gagaatcgat
     1501 cttggacgac cacggtgacg tgcttggcag ttaggtcacc gagggtgcgg gagattcccc
     1561 gagtgtccgt gtcccggcga gcctcccgca gcgctgtctc ctgccggacg cgatctcgct
     1621 cgcccatgat cagccgtttt gctccggcca acgcgatcgc ggcggcaagc tcgcgttgcg
     1681 ccgtcaccaa tccgtgaact ctctggttga acgtgtcggt gtctatcagt atggcgtcgg
     1741 ccgcgtactg ggcggacttg gctcgcaggc tctgctcaag ctcatcgttt ggctcatcag
     1801 gcagggcgtc gcgccccact tccacagcat ctcggcgctc cccccagact cggtattgcg
     1861 ccgcagcggc cgctgctagg ggttcgtcga gatcccgcag cgtcagagcc agcgagtcgc
     1921 cgtcacggcc ggcggcgagc cactcggaca tctctgtgtc cagtcgttcc tcaagtcggc
     1981 gagcaatgct ctcgcttgtg tccgtcatca ggcgctgaaa tcgggtgagt cgatcgcggg
     2041 ctgctgagtc gaggtgctgc tgacacagcg gacaggcagc cccctcatct gtgttgggaa
     2101 actcatgctc gggatagacc tcggtggcgg agtaccgttc cgcagcgagc cagagggtgc
     2161 gccagagagc tgaaccgacc ccgtccaatg gatctgagat ggtccgctca gccgccatcg
     2221 cgcgggcaac ttggcgagcc tcggctgcct ccgactgcag ggtgcgcagc tgacgctccg
     2281 cgacggcgtc gaggctcgtg cgggcttcgg ccagaagctg cgccgcccgc aagtatgagc
     2341 cgctcagttc ctcgatgcgc gtgcgctccg tcgtggggtc ggtcgccctc agtcgggcct
     2401 cttcggaccg agccctctcg atctccgcct cggcatctcg ggagaccgtg caagcgctgg
     2461 caatctcatc gtcggtagta tcagcgttta ggctgcttag aaactgcgct gcgggggtgg
     2521 ccgcatccag ctcaggcaga gcgacgcggt tccgtgacag gtccgcgagg cgtgagtcaa
     2581 tagccgcccg aaccgtgtcg catacacgaa tgaggccatc aaagagggca agcgcggaag
     2641 gacgatagga ggcttctccc tctttgctga cgtacaggtc gccacagcgt tcgtcgtaga
     2701 aggtgatttg ttgtagcgaa ggaggggcct catctccgag acgcaccgtc gcgaccgtgc
     2761 ccccctcgaa gaacgatagg gccgcagtca cagagccgct ggtggacctg aagatgtcag
     2821 gcagcacctc cgccgcgtct ctggtcctta ccaggctctt caccaggcgc gcgtagccgg
     2881 acttcccgct gccgttgttg ccatagatga cagtcacacc gctgggcgcg aacgtgagcg
     2941 tttgtccggc caccagccgg tttacattat ccactccgtg gattgagacg agcgagaccg
     3001 ccgaggcggg ctgttctagg ggctgttgaa tgcccgccag ccacccaccg ggtggcgtgg
     3061 ctggtggtgt cgacaacatt gagtggacga gggcttcgta gtcgaaggcg gcatcgtctc
     3121 cggtggacgc cagggtcacc aaagcctgtt gctgccacca gggcctcgtc gccgcccacg
     3181 aggtgatgtc ggaatacaca gtcatgtctt gagcctccgc ttcatccgat ccattcttgg
     3241 cgaaactcgt cttaggtgca ggagaggtat cggacgaggg agacgtggaa gtcggggttg
     3301 gagaagaaca ggtcgaagac caccggcaac tggtccctag cgtctggcac cgacacgacc
     3361 cttcgaggcc aggccagcga gcaagcccga ggttgcggtg gcgtacaggc caaacaggtg
     3421 cgactgtcgc tggagctctg ttgcgtcatc tcgaatgttg aaggcggcgt tcacctcgtg
     3481 atcgagggcc gcatgcgcgc tggccaacac ttcgcccatt gcgcccggcg cgtacatggc
     3541 tgccagtgag ccctcgctgt gacgagatct ggcgagtagc actgcttctc ctgctccggc
     3601 gatggccgag cgctgagcag actggagtgt cggaacgggg aacgtgttcc aagtaaggag
     3661 cttgttgaag cggaggtcgg acttgatccg cccaccgacc gctcgctgcc atgccacgaa
     3721 catcgacgac gatataaggg cgaaaaccag gccgtcggga tcaggtgcga cgaagttggc
     3781 attgcttgcg atgacctctg ggccaaagtg gccagcgagg aagtagggcc gctcttcgga
     3841 aacatgggcg gggatgcaga ggtacgacgt gtccggctgt gcgatttgtc ggaagaggtg
     3901 cgccgtgctg gcagcctgac gcgtgctcgc agcgtcgctg tccaggcgga atgtacgcac
     3961 gccctcaaca cgctcgcgca ggaccgggct cccctggata tcggtcgctg gcgcaccaac
     4021 gagccaaagg cagtagcggt cgccgccgtg caggagctct cgtgccccga cgtagggtcg
     4081 caggtatttt cgcgcgatgg ggtccgactc gacgccagtt cgttcatcgc cctcgacaat
     4141 cagccaacct ccatcggtcg gcttgttccc gtaggccacc ttcccaacct gagggttaag
     4201 tggatgtccg gacgggtcga caataacggg gggcccgaag gtcaggtatg gagtgatcga
     4261 cccgttcgtc ggcaactcgg tgggacccgc cttggaactg gggtagtcga agagacgcgc
     4321 gggtggcccg cctcggtcga aaccgatgat cgtgcagtga acagccgcct tgcccgaggc
     4381 gtccgatgtc catgggaacg ttcggtgtgc gaacctgatc cgccagtctg ccccgagaat
     4441 cggccggaac agtggcccga caggctcccc ctgtgtgatc gagttagtgg agacgtaggc
     4501 gaatcggccg ggcgcttccc cgaagtactt aagcgcgcgg gcgtaccatc cggtgacgta
     4561 gtcgagactg ccgtggtagc cggaccccca cacccactga aggtctgccg tctgctcggc
     4621 agaccttcct gagatgccca ggaacggcgg atttcccgcg acgacaacgt tctgggtggg
     4681 aggcaggagc atctcccact gagtgcgcaa cgcgttaccg acaacgattt tcgcctcgcg
     4741 ctggatggga aggcgctctg gcgcttgccc aaaacgctcc tggagcttga ggtcacactg
     4801 acggtccatc atgaacatcg cggtctcggc gattcgcacg ggccactcgt cgatctcgat
     4861 gccgtagaag tgatcgagcg tgaccttgag gtcggacttc gcgtcgagcg agagctggtg
     4921 cttgtcgcgg aggtcgtaca gcgcctccat gacctggagc tcgatctcac gtagctcgcg
     4981 gtaagccacg atgatgaagt tgccgcatcc gcacgcgggg tcaatgaacc ggatgtcgcc
     5041 gag
//
LOCUS       DAOMZY010000262         5034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_15656_length_5034_cov_4.643503, whole genome shotgun sequence.
ACCESSION   DAOMZY010000262 DAOMZY010000000
VERSION     DAOMZY010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15656_length_5034_cov_4.643503"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PLK64_13160"
     CDS             <1..532
                     /locus_tag="PLK64_13160"
                     /inference="COORDINATES: protein motif:HMM:NF038065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HPV80740.1"
                     /translation="RLADWFSYFTIWSNIVVAVVTSLLAWGRPRVGAALLAARLDSVL
                     LITITGLVYAVVLAPVSVQRGWENVSNTLLHQVTPVLTVLVWVVVGPRGWVSRRTVLH
                     SLAIPIAWLVVMLLHGAFVGAYPYPFVDVAVLGYARALGNVAGILVLGLLMAGGFLAA
                     ERGLARHWRRAPSLRE"
     gene            537..1796
                     /locus_tag="PLK64_13165"
     CDS             537..1796
                     /locus_tag="PLK64_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HPV80741.1"
                     /translation="METLDIDHLGTVIDSRRTDLRVVTSGNCSTPLTVLGKVDTLLAE
                     YRLHLLNAPRGIPQRDGVIPESAFVGVGMRHHPRLVYFPMRLSGVPFLYQRRLRVDVV
                     IAHTSAPIAGKVSLGLEINVLPGAIAACKAAGGIVIAQVNRQMPYTFGDGELPLDVFD
                     AIVEVDEPVLNLATSEADGTASGGGMSRADSARIIGDLVSRRVADGATLQLGIGEIPD
                     AALDGLRGKRGLGIWSEVIGDGVLELDQAGALDPARTITASALMGSAALYAWAHRNER
                     LRVLRTETTNSPSIIATQPLMTSINTALQVDLYDQANAASVNAKVYSGFGGQSDFTVG
                     ALHAPRGQALMALRSWHPKADVSTIVPLIEAPVTSFQHTAVITEQGVAEIAWRDQKSQ
                     AVQLIEHAAHPAARDDLWEEAQELGLL"
     gene            complement(1846..2232)
                     /locus_tag="PLK64_13170"
     CDS             complement(1846..2232)
                     /locus_tag="PLK64_13170"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HPV80742.1"
                     /translation="MQQSVSFKVYYEDTDSLGVVYYANYFKFMERGRSEFLTAQGRSV
                     ADWNAAGFLLVVHSVQATFKRPAVLGDTVDIVTSFTLPSVYRGRFAQRVERAGELLVD
                     GIVDVVCLDAAQNLIEFPQQLRALAD"
     gene            complement(2242..4188)
                     /locus_tag="PLK64_13175"
     CDS             complement(2242..4188)
                     /locus_tag="PLK64_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HPV80743.1"
                     /translation="MTSTAPAAVVDATASILADLSPERRELFELRLHRWWDDLLDALE
                     TLYGERAFDVAQRLVAHAAAAYRDRDPELHRLDERRILAPDWLQDPRMFGYATYADRF
                     AGTLEAVGGKVDYLEELGVTYLHLMPLLRPREGDNDGGYAVASYRELRPDLGTIEDLK
                     ALSKLLRDKGISLVLDLVLNHVAKEHDWAQRARAGESHYRDYFYVYPDRTEPDAYEAT
                     LPEVFPDFAPGNFTWDDAMGGWVWTTFNSWQWDVNWANPDVFCEYADIIFFLANAGVE
                     VVRLDAIAFTWKRLGTNSQNQPEVHALTQALRAIARIACPAVAFKAEAIVGPRDLVQY
                     LGQGEHHGKVSDLAYHNSLMVQAWSMFATQDVRLTAHALSALPPVPTTATWISYLRCH
                     DDIGWAIDDGDAAAVGLNGFAHRAFLSDWFIGAYPGSSARGLTFQANEATGDRRISGT
                     AAALTGLDAAREVGDQAAVDRAISRILLGHALIAGFGGVPVIWSGDELGLPNDPVWAG
                     EPGHEDDNRWVHRARMHWEVAARRHTPGTAEHRVFGGIAHIAHTRARLPHLHASVPSS
                     VLGVWDSGVLVFARRHPVGTMLAAYNVTDSWRPVPGWVLRQEGLSSGTEALSGAHVRI
                     EADDNAWMPPYAVWWLVDHIPAHA"
     gene            4413..>5034
                     /locus_tag="PLK64_13180"
     CDS             4413..>5034
                     /locus_tag="PLK64_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPV80744.1"
                     /translation="MTIYGRNPVLEALGDPSLTVDKVLMADSAHGPHVSEIRSAARAR
                     GVPVQLVGAERVKKIAGNGKQDQGVCADVVAPRMRPLDLALADGLRGPIIVLDGITTP
                     ANVGMILRTATAAGFSGIVVPRRGVASIDPLVVKASAGVAFKAPILKVATAVEAVRLL
                     LDARYRVAGLDADGPADLFAADLPGDTAYVMGGETAGLGAEVSELVD"
BASE COUNT          769 a         1724 c         1755 g          786 t
ORIGIN      
        1 gcggttggcc gactggttct cctacttcac gatctggtcg aacatcgttg tcgccgtggt
       61 gacctcgctg ctcgcctggg gccgtcctcg ggtgggcgcg gccttgctcg cggctcgtct
      121 cgactcggtc ctgctcatca ccatcaccgg cctggtgtat gccgtggtgc tggctcccgt
      181 ctcggtccag cgggggtggg agaacgtctc caacaccctt ctgcaccaag tgactccggt
      241 gctgacggtg ctggtgtggg tggtcgtcgg accccgcggg tgggtgagcc ggcgcactgt
      301 ccttcactcg ctcgccatcc ccatcgcttg gctcgtcgtc atgctgctgc acggggcgtt
      361 cgttggcgcc tacccctacc ccttcgtgga cgtcgccgtc ctgggctatg cccgggccct
      421 gggcaacgtc gccgggatcc tcgtgctggg cctgctcatg gccggtggct tcctcgctgc
      481 ggagcgagga ctcgcccggc attggcgtcg cgcgccgagt ttgcgagagt gagtgcgtgg
      541 agacgctcga catcgaccac ctcgggactg tcatcgacag ccggcgcacc gacctgcgcg
      601 tcgtcaccag cggcaactgc agcactccgc tgaccgtcct cggcaaggtc gacaccctcc
      661 tggcggaata ccggctgcac ctgctcaacg cgccgcgcgg cataccgcag cgcgatgggg
      721 tgatccccga gtcggcgttc gtcggggtcg ggatgcgtca ccacccccgt ttggtctact
      781 tcccgatgcg gctctcaggg gtgccgttcc tctatcagcg gcgcctgcgc gtcgacgtcg
      841 tcatcgccca cacctccgcg ccaatcgccg gaaaggtgtc cctcgggctt gagatcaacg
      901 tcctgcccgg agccatcgcg gcgtgcaagg ccgccggcgg gatcgtcatc gcgcaggtca
      961 accggcagat gccctacacg ttcggtgacg gggaactgcc gctggacgtc ttcgacgcca
     1021 tcgtcgaggt cgacgagccg gtgctcaacc tggccacgtc agaggccgac ggcacggcca
     1081 gcggcggcgg gatgtcgcgc gcggacagtg ctcggatcat cggcgacctg gtgtccaggc
     1141 gcgtcgctga cggtgccacc ctgcaactgg gcatcggcga gatccccgac gcagcgctgg
     1201 acgggctgcg cggcaagcgg ggcctgggga tctggagtga agtcatcggc gatggggtcc
     1261 tggagctgga ccaggccggg gcgttggacc cggcccgcac catcaccgcg tcggccctca
     1321 tggggtcggc cgcgctctac gcctgggcgc accgcaacga gcgactgcgt gtcctgcgca
     1381 ccgagaccac caactcgccg tcgatcatcg ccacccagcc tctgatgacg agcatcaaca
     1441 cggcgctgca ggtcgacctc tacgaccagg ccaacgcggc cagcgtcaac gccaaggtct
     1501 attcgggctt cggcgggcag agcgacttca ccgtcggtgc cctgcacgcg ccgcgcgggc
     1561 aggccctcat ggcgctgcgt tcctggcacc ccaaggccga cgtgtcgacc atcgtgccgc
     1621 tcatcgaggc accggtgacg tccttccaac acaccgccgt catcacggag cagggtgtgg
     1681 cggagatcgc ctggcgcgac cagaagagcc aagcggtgca actcatcgag catgccgcac
     1741 acccggccgc ccgcgacgac ctctgggaag aggcccagga actcggcctg ctctgagctg
     1801 ggccggctga cgcagtcggg ccggctgccg cagtacagcg gggcctcaat cggcgagggc
     1861 gcgcaactgc tgcgggaact cgatgaggtt ctgggccgcg tccaggcaca cgacgtcgac
     1921 gatcccgtcg acgagcaact cccctgcccg ttccacccgt tgggcgaacc ggccgcggta
     1981 gaccgacggc aacgtgaatg acgtgacgat gtcgacggtg tcacccaaca cggccggtcg
     2041 cttgaaggtc gcctgcaccg agtgcacgac caacaggaag cctgcggcgt tccagtcggc
     2101 caccgaccgg ccctgagcgg tcaggaactc cgaccggccc cgctccatga acttgaagta
     2161 gttggcgtag tagacgaccc cgaggctgtc ggtgtcttcg tagtagacct tgaacgagac
     2221 gctctgctgc acaccccgac cctatgcgtg cgccgggatg tggtcgacca gccaccacac
     2281 ggcatacggg ggcatccagg cgttgtcgtc ggcctcgatc cgtacgtgcg cgcccgagag
     2341 ggcctccgtc ccgctggaca ggccctcttg ccgcaggacc cagcccggca cggggcgcca
     2401 cgaatcggtg acgttgtagg cggcgagcat ggtgcccacc gggtggcgcc gcgcgaacac
     2461 gaggacaccc gagtcccaga cgccgaggac gctcgacggc accgacgcgt gcaggtgcgg
     2521 caaccgggcg cgggtgtgcg cgatatgcgc gatcccgccg aacacgcggt gctcggccgt
     2581 ccccggggta tgccgtcgcg ccgccacctc ccagtgcatc cgggctcggt ggacccaccg
     2641 gttgtcgtcc tcgtggcccg gctctccggc ccagaccggg tcgttgggga gccccaactc
     2701 gtcaccgctc cagatcaccg gcacccctcc gaagccggcg atcagagcgt gccccaacag
     2761 gattcgcgag atggcgcggt cgaccgctgc ctggtcgccg acctcccgtg cggcgtcaag
     2821 ccccgtcaac gccgccgcgg tgccactgat ccgccggtca ccggtggcct cgttggcctg
     2881 gaaggtgagg ccgcgggcgg acgagcctgg ataggcaccg atgaaccagt cggagaggaa
     2941 ggcccggtga gcgaagccgt tgagcccgac cgcagcggcg tcaccgtcgt cgatggccca
     3001 gccgatgtcg tcgtggcagc gcaggtagga gatccaggtc gcggtcgtcg ggaccggcgg
     3061 cagcgccgag agggcatggg ctgtcagccg cacgtcctgg gtggcgaaca tcgaccaggc
     3121 ctgcaccatg agcgagttgt ggtaggccaa gtcgctgacc ttgccgtggt gctcaccctg
     3181 accgagatac tgcacgaggt cgcgcgggcc gacgatcgcc tcggccttga atgcgactgc
     3241 gggacaagcg atccgggcga tggcacgcag cgcctgggtg agggcgtgca cctcgggctg
     3301 gttctggctg ttggtgccca gccgcttcca cgtgaacgcg atcgcgtcca gccggacgac
     3361 ctcgacgccg gcgttggcga ggaagaagat gatgtcggcg tattcgcaga agacatccgg
     3421 gttggcccag ttgacgtccc actgccagga gttgaacgtc gtccagaccc acccgcccat
     3481 cgcgtcgtcc caggtgaagt tgccgggggc gaagtcgggg aagacctcag gcagggtcgc
     3541 ttcgtaggcg tcgggctcgg tccggtcggg atagacgtag aagtagtcgc ggtagtgcga
     3601 ctcccccgcg cgggcccgct gggcccagtc atgttccttg gccacgtggt tgagcacgag
     3661 gtcgaggacc aggctgatgc ccttgtcgcg caggagcttc gacagcgcct tgaggtcctc
     3721 gatcgtcccg aggtcggggc gcagttcgcg gtaggaggcc acggcatacc cgccgtcgtt
     3781 gtccccctcg cgagggcgca gcagcggcat gaggtgcagg taggtaacgc cgagctcttc
     3841 gaggtagtcg accttgccgc cgacggcttc cagtgttccc gcgaagcggt cggcgtaggt
     3901 ggcgtagccg aacatccgcg ggtcctgcag ccagtcgggc gcgaggatgc gtcgttcgtc
     3961 cagtcggtgc aactcgggat cgcggtcccg gtatgccgcg gccgcatggg caaccagccg
     4021 ctgcgccacg tcgaaggccc gctcgccgta gagggtctcc agcgcgtcga ggaggtcgtc
     4081 ccaccatcgg tgcagccgca actcgaagag ctcacggcgc tcgggcgaaa ggtcggcgag
     4141 gatcgacgca gtggcgtcga ccaccgccgc aggtgccgtg gaggtcatcg ggaggagccc
     4201 tcccgccgaa gacggcgacg cgggacgcgg tcggtcatgg ctccctcaga ctcggtcaga
     4261 aacggacgcg attgcgggct cgcggagccc gagtcacagc atacgtaagc gtttacacgt
     4321 gccgctagcc ctcactcgga cgagtgaacc ggttggatag gcgggtgacc ggacccaacg
     4381 cttcgacacc gatctcaccg aaagaccggt ttgtgacgat ctacggccgc aaccccgtgc
     4441 tggaggccct cggtgacccc tcgctgaccg tcgacaaggt cctgatggcc gactcggccc
     4501 acggcccaca cgtgtcggag atccgctcgg ccgcgcgcgc ccgcggcgtc ccggtgcaac
     4561 tcgtcggcgc cgaacgggtc aagaagatcg ccggcaacgg caagcaggac cagggcgtgt
     4621 gtgccgacgt cgtcgccccg cggatgagac cgctggacct cgccctggcc gacgggctga
     4681 ggggcccgat catcgtcctg gacggcatca ccaccccggc caacgtcggg atgatcctgc
     4741 ggacggccac ggccgcgggc ttctcgggga tcgttgtgcc gcgccgggga gtcgcgtcga
     4801 tcgacccgct cgtcgtcaag gcgtccgccg gcgtcgcttt caaggccccc atcctcaagg
     4861 tggcgacggc ggtcgaggcc gtccggttgc tgctggatgc gcgctaccgg gtcgccgggc
     4921 tggatgccga cgggccggcg gacctgttcg cggcggacct gccgggcgac acggcatacg
     4981 tgatgggcgg ggagacggcc gggctgggag ccgaggtgag cgagttggtg gaca
//
LOCUS       DAOMZY010000263         5027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_15682_length_5027_cov_5.246782, whole genome shotgun sequence.
ACCESSION   DAOMZY010000263 DAOMZY010000000
VERSION     DAOMZY010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15682_length_5027_cov_5.246782"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PLK64_13185"
     CDS             complement(<1..295)
                     /locus_tag="PLK64_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80745.1"
                     /translation="MTFSEPSSSREPSAPDAPHVRAAVIAVYAVFLLAGVQFAAFAAR
                     IPQVKDLLGLSAGELGVTLLAVSAGSILGLPAAGWVVHRFGAARTVLAGVIASV"
     gene            complement(356..1720)
                     /gene="tig"
                     /locus_tag="PLK64_13190"
     CDS             complement(356..1720)
                     /gene="tig"
                     /locus_tag="PLK64_13190"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPV80746.1"
                     /translation="MKSALETLSPTRVKLTIEVSAEEFQPSLEQAYKTIGSQIQVPGF
                     RRGKIPARIIDQRLGKGAVLQEAVNDALPTFYGQALEEHSVRPLGQPEVDVTEVPLDA
                     GQGLTFTAEVDKRPEIELPDYSTLAVEVDAIEVGDADIEERLTTLRQRFGTLKGVERA
                     AATGDFVSIDLSAQIDGEEIDTVTGVSYEVGAGNMLEGMDEALVGMTAGETKTFEAPL
                     AGGDHAGAAAECTVTVQSVKERELPELDDEFAQLASEFDTLEELKADLVKDAERGKRF
                     GQGMQARDKVMEALLAAVEIPIPEGVVNDEVHSHLEGEGRLEDDEHRAEVDAETRKGL
                     AAQFLLDAIVEKEEIRVEQPELIEYLLMSAQQYGMDPNQFAQAIDAQGQIPAMVSEVA
                     RRKALAKVLEQASVTDSNGAAVDLEAVMAPRAPFGDDHEGHDHEGHDHEGHDHDHDGH
                     DHEH"
     gene            complement(1996..2072)
                     /locus_tag="PLK64_13195"
     tRNA            complement(1996..2072)
                     /locus_tag="PLK64_13195"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2036..2038),aa:Pro,seq:tgg)
     gene            2214..2287
                     /locus_tag="PLK64_13200"
     tRNA            2214..2287
                     /locus_tag="PLK64_13200"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2246..2248,aa:Gly,seq:tcc)
     gene            complement(2256..2927)
                     /locus_tag="PLK64_13205"
     CDS             complement(2256..2927)
                     /locus_tag="PLK64_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPV80747.1"
                     /translation="MPEQRVRTFHARRGRRSSLTESRLATLLPRYAVPAAVLAPPDTP
                     GRPPRVVLEVGCGHGAAAVGYAATHPDTHLIALDVHPPGVARMLAAAADEGLTNLSAE
                     LADAVEFLADRVPEGGLDAVHLFFPDPWPKARHHRRRFVSPQTLDLLASRLTPTGHVL
                     VATDIADYAAYVRRVVADHGTFVARDVPRPTWRPLAGYEAKGLDAGRAITELVLERAT
                     RIELA"
     gene            3053..3265
                     /locus_tag="PLK64_13210"
     CDS             3053..3265
                     /locus_tag="PLK64_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005269362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HPV80748.1"
                     /translation="MGLFDEIKDKAEELKDKAVELVQGNSDKVDEAVEKAGDLIDEKT
                     GGQFSDKVDMAQEKAKEVADDLDQAN"
     gene            complement(3373..4500)
                     /locus_tag="PLK64_13215"
     CDS             complement(3373..4500)
                     /locus_tag="PLK64_13215"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HPV80749.1"
                     /translation="MTAQTARREVDPGSTRVSVLRAEPWPARLRVVLQARQPVRAFGL
                     VVAGWVLLACMVAWPLNVAWSMYAPLVVLAGLFLTPGWFAVVSASYAVCLGISAFMVR
                     GWSVLQGFTLLALALTMTLMVMRTRSRERLGVQGNSGEDMLVDLRDRLLALGDMPSLP
                     TGWHAESAIRSAYGDKFSGDLAVTSLSPDGHRLEVALVDLSGKGRTAGTRSLLCSGAI
                     GGLLGQVEPERLLPAANHYLVRQEWPEGFATAVHLAVDLRTGDFTLGNAGHPAPIQFT
                     AGSGRWSVLESAGGPVLGIIPEIQFPRSAGRLGRGDALVLYTDGVIEARGHDLSRGLD
                     RLLGNAERLITSGFAGGARRLCADAQAGDSDDRAVVVIWRD"
     gene            complement(4509..4973)
                     /locus_tag="PLK64_13220"
     CDS             complement(4509..4973)
                     /locus_tag="PLK64_13220"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HPV80750.1"
                     /translation="MRVHLGGDHAAYDLQRALVDHLTAAGHEVIDHGPLAYDAMDDYP
                     VFVLRAAQAVAADPGSAGIVLGGSGNGEVMAANKVPGIRAAYAASIELAELAREHNDA
                     QIISMGGRFTDIETGCAIADAFLATDFSGDERHARRLAMVSRFEADGSLPEG"
BASE COUNT          778 a         1719 c         1808 g          722 t
ORIGIN      
        1 ggaccgaggc gatgactccg gccaggaccg tgcgagcggc gccgaaccga tggacgaccc
       61 accccgcggc cggtagcccg aggatcgagc ccgccgagac ggccaggagg gtgactccga
      121 gctcgccggc gctcaggccc agcagatcct tgacctgcgg gatccgggcc gcgaacgccg
      181 cgaactgcac ccccgcaagg aggaacacgg catacaccgc gatgacggcc gcgcgcacgt
      241 gaggtgcgtc gggcgccgac ggttcgcgag aagaggacgg ctcagagaac gtcatgaggg
      301 tgaagtgagg tgacccgggt cagcgctgcg acccggtcgg caaggttcgc cgggctcagt
      361 gctcgtggtc gtgaccgtcg tggtcgtggt cgtgaccctc atggtcgtga ccctcgtggt
      421 cgtggccctc gtggtcgtcg ccgaacggcg cgcgcggggc catgacggct tcgaggtcga
      481 cggccgcacc gttgctgtcg gtcaccgagg cctgctcaag caccttggcc aaggccttgc
      541 gccgggcgac ctcgctgacc atcgcgggga tctgaccctg cgcgtcgatg gcctgggcga
      601 actggttggg gtccatgccg tactgctggg ccgacatgag caggtattcg atgagctcgg
      661 gctgctcgac gcggatctct tccttctcga cgatggcgtc gaggaggaac tgcgcggcca
      721 ggcccttgcg ggtctcggcg tcgacctcgg cgcggtgctc gtcgtcctca aggcgcccct
      781 cgccctcgag gtggctgtgg acctcgtcgt tgacgacgcc ctcggggatc gggatctcga
      841 cggcggccaa cagggcctcc atcaccttgt cgcgggcctg catgccctga ccgaaccgct
      901 tgccgcgctc ggcgtccttg acgaggtcgg ccttgagctc ctccagggtg tcgaactcgc
      961 tcgccagctg ggcgaactcg tcgtcgagct cggggagctc gcgctccttc acggactgga
     1021 cggtgacggt gcactcggcg gctgcaccgg cgtggtcgcc gcccgcgagc ggggcctcga
     1081 acgtcttggt ctcgcccgcg gtcatcccga cgagcgcctc gtccatgcct tcgagcatgt
     1141 tgccggcgcc gacctcgtag gacaccccgg tgacggtgtc gatctcctcg ccgtcgatct
     1201 gggccgacag gtcgatcgac acgaagtcgc ccgtggcggc ggcgcgctcg acgcccttga
     1261 gggtgccgaa acgctggcgc agcgtggtga ggcgctcttc gatgtcggcg tcgccgacct
     1321 cgatcgcgtc gacctcgacg gccagggtgg agtagtcagg cagctcgatc tcggggcgct
     1381 tgtcgacctc ggcggtgaag gtgaggccct gaccggcgtc gagcggcacc tcggtcacgt
     1441 cgacctcagg ctgacccagc gggcgcaccg agtgctcctc gagggcctgc ccgtagaagg
     1501 tcgggagcgc gtcgttgacg gcctcctgca ggacggcgcc cttgcccagg cgctggtcga
     1561 tgatccgcgc cgggatcttg ccgcgccgga accccgggac ctggatctgg ctgccgatgg
     1621 tcttgtacgc ctgctccagg ctgggctgga actcctcggc ggagacctcg atggtcagct
     1681 tcacccgggt cgggctgagg gtctcgagcg cactcttcac tgcggatact ccagatcggg
     1741 aggaaggtat gccgtccggc tagctcgcgc ccgtgcgtgc acgaggcgtg gcggccggcg
     1801 cggacgacgg cgggcaatcc ggtccatgct agccgcctgt gtgcggccgg gccgaatccg
     1861 agagcggccg acggcctggg agctggcccg ggcggcggtc acgacacatg aagtcgccga
     1921 tgaagtcgtc gctactgctg ttccaacgac agccacggcg acttcatggg cgcccccacg
     1981 cgtcgctgga ccagatggtc ggggtagccg gattcgaacc gacggccttc cgctcccaaa
     2041 gcggacgcgc taccaaactg cgccataccc cggtcggtgg gcacacggca taccgtggcc
     2101 gacgcaccga gcccgctacg ggacgcgcgg acgtggtggt tcgcgcgagc cgggcgggcg
     2161 ggactaggct agccccccgt ggtggccccg tcacaatgcg acggccaccg cacgcgggcg
     2221 tagctcaatg gtagagcccc agtcttccaa actggctacg cgagttcgat tctcgtcgcc
     2281 cgctccagca ccagctcggt gatcgctcgc ccggcgtcca ggcccttggc ctcgtaaccg
     2341 gccagcggtc gccacgtcgg acgcggcaca tcccgcgcca cgaacgtccc gtggtcggcc
     2401 accacccgcc gcacgtatgc cgcgtagtcg gcgatgtcgg tcgccacgag gacgtgcccg
     2461 gtcggggtca ggcgggaggc caacaggtcg agggtctgcg gcgacacgaa ccgacgccgg
     2521 tggtgccgag ccttgggcca cgggtcgggg aagaacaggt gcaccgcatc gagaccaccc
     2581 tcgggcactc ggtcggcgag gaactccacc gcgtcggcca actcagccga gaggttggtc
     2641 agcccctcgt ccgcggcggc agcgagcatc cgggcgacgc ccggcgggtg cacgtcgagg
     2701 gcgatgaggt gcgtgtcggg gtgggtggcg gcatacccga cggcggcggc gccgtggccg
     2761 cacccgacct cgaggacgac gcgtgggggc cgcccgggcg tgtcgggagg ggcgaggacc
     2821 gcggcgggca cggcataccg gggcagcagg gtggccaacc ggctctcggt gaggctggag
     2881 cggcgccccc gtcgcgcgtg gaaggtccgg acccgctgct ctggcacgcg cacagggtat
     2941 gccgctcgcg ctcgccaggc acgcggcggg aacttccggt cgcgtgggct cgcttatggt
     3001 gggtcaaggc cgtccgcacg ggcggcgacc acgagtgggg aggagggcca acatgggcct
     3061 tttcgacgag atcaaggaca aggctgagga gctcaaggac aaggctgtcg agcttgtcca
     3121 gggcaacagc gacaaggttg acgaggctgt ggagaaggct ggcgacctga tcgacgagaa
     3181 gaccggtggt cagttctcgg acaaggtcga catggcgcag gagaaggcca aggaagtcgc
     3241 tgacgacctg gaccaggcca actgagcttt cgcttcagca gcgccgacgg ccgccgctca
     3301 ccttcgggtg agccgcggcc gtcggtgtgt ttagaggggt gtggggcggc gcggatgggc
     3361 gccgtgggcc ggtcagtcgc gccagatgac gacgacggct cggtcgtcgg agtcgccggc
     3421 ctgtgcgtcg gcgcacaacc gacgagcgcc cccggcgaac ccggacgtga tcagtcgctc
     3481 ggcattgccc aacagacggt ccaagccccg gctcagatcg tgaccgcggg cctcaatgac
     3541 cccgtcggtg taaagaacca gcgcgtcccc gcggccgagc cgccctgcgc tacgcgggaa
     3601 ttggatctcc gggatgatcc ccaggaccgg gccaccggcc gactcgagga ccgaccagcg
     3661 cccagagcct gcggtgaact ggatcggcgc cgggtgcccg gcattgccga gcgtgaagtc
     3721 gcccgtgcgc aggtcgacgg ccaggtgaac ggcggtcgcg aaaccttccg gccactcctg
     3781 gcgcacgagg tagtggttgg ccgcgggcag cagccgctca ggctccacct ggccaagcaa
     3841 cccgccgatc gctcccgaac acagcaacga ccgggtgccc gcggtgcgtc ccttcccgga
     3901 caggtcgacg agcgcgacct ccaggcgatg accgtcgggc gacaacgagg tcacggccag
     3961 atcgccggag aacttgtcgc cgtaggctga ccggatggcc gactcggcgt gccaaccggt
     4021 cggcagcgac ggcatgtcgc ccagtgccag caaccgatcg cgcaggtcga cgagcatgtc
     4081 ctcaccgctg ttgccctgca ccccgagccg ctcccgtgac cgggtgcgca tcaccatgag
     4141 ggtcatcgtc agcgcgagcg ccaacaaggt gaatccctgc agcaccgacc acccgcgcac
     4201 catgaacgcc gagatcccca ggcacacggc atacgaggcg ctgaccaccg cgaaccagcc
     4261 aggggtgagg aacaacccgg cgaggacgac gagtggcgcg tacatcgacc aggcgacgtt
     4321 gaggggccag gcgaccatgc aggccagcag gacccagccg gcgacgacca gcccgaacgc
     4381 gcgcaccggc tgcctcgcct gcagcaccac ccgcaggcgc gctggccacg gctcggcacg
     4441 cagcaccgac accctggtcg accccgggtc gacctcgcga cgtgccgtct gtgcagtcat
     4501 ctggggcgtc agccttccgg caggctcccg tcggcctcga agcgactcac catcgccagt
     4561 cggcgggcgt gccgctcgtc accggagaag tccgtcgcga ggaacgcatc ggcgatcgcg
     4621 cagccggtct cgatgtcggt gaaccggccg cccatcgaga tgatctgggc gtcgttgtgc
     4681 tcgcgggcca actcggccaa ctcgatgctc gcggcatacg cggcgcggat cccggggacc
     4741 ttgttggccg ccatgacctc gccgttgccg gacccgccga ggacgatgcc cgccgatccc
     4801 gggtcggcag ccacggcctg ggccgcccgc agcacgaaca ccgggtagtc gtccatcgcg
     4861 tcgtaggcga gcggtccgtg gtcgatgacc tcgtgtccgg ctgccgtcag atggtcgacc
     4921 agcgcgcgtt gcaagtcgta tgccgcgtgg tcgccgccga ggtgaactcg catgtgtggg
     4981 aatcgctttc agtcgaagat ggggccgatg gagcgggtgc gcttgag
//
LOCUS       DAOMZY010000264         5027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_15683_length_5027_cov_4.525744, whole genome shotgun sequence.
ACCESSION   DAOMZY010000264 DAOMZY010000000
VERSION     DAOMZY010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15683_length_5027_cov_4.525744"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1477
                     /locus_tag="PLK64_13225"
     CDS             <1..1477
                     /locus_tag="PLK64_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019159910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPV80751.1"
                     /translation="TKSRRNTEFLLLLFAVAIVLFAWVDTEVANRGAVPAGLVAIGGG
                     LLVLVLLFHLVLRWKASYADPLLLPIATLLNGLGLVMIHRLDLAAGDYGFDGDAFRQL
                     IWTGVSIIVAGALLWTLRDHRTLRRYTYTAMALGVLLLVLPLVPGLGSEQLGSRIWIR
                     VGPMSFQPGEIAKIALAVFFAGYLVQARDVLSLAGRKVLGFTFPRGRDLGPIMVAWLV
                     SVAVLVFEKDLGSSLLFFGLFVAMLYVATERVSWIAIGLMLFAAGAYAAYLLFPHLQR
                     RVMLWLDPFSSDSLAQSDQLAKGLMGMAAGGLFGTGLGKGRPWLVAFAESDFIVPSLA
                     EELGLTGLFAILLLYGLFVQRGLRTALGVRDGFGKLLALGLAFSVALQVFVVLGGVTR
                     VIPLTGLTTPFMSLGGSALLANWSITALLLRISDQARRPVLDSDGLHTIPRRKPTPEA
                     VAAPAATPAASPALAPAVATGPAAASAPPAVAAPTEAVPRP"
     gene            1474..2931
                     /locus_tag="PLK64_13230"
     CDS             1474..2931
                     /locus_tag="PLK64_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPV80752.1"
                     /translation="MNTPILRMSRLVGALFAILLISATIIQFVQAPSLRARPDNRRTL
                     LDNYSRDRGSILVGDTAIATSTPSEGELAYLRTYPQGPLYSHVTGYYSYVYGAGGGLE
                     GSSDGLLSGSSDALFYKRIADLLTGKQPTGASLELTLNAKAQEAASKALGNQRGAVVA
                     LDPRTGAILALVSHPEYDPNTLANHDSNAQKTAWDGMLDDRTRPLVDRTISGNLYPPG
                     SVFKIVTTAAALSTGAVNETTQIPGPAVMDLPQTTVGLPNHDGKSCGPGDLTTLQHAL
                     EISCNTAFGWIGLKVGGDALRDQAAKFGFGESLRIPMRVTPSVFPSSLNPPQTAQSAI
                     GQFDVRVTPLEVAMVSAAIANRGVVMRPYLVQQVVGANLEILDTHTPEQMSVAVTPEI
                     AAIISRMMVGVVDQGSGKPARINGVSVAGKTGTAEQGNGLPAHAWFTSFAPADNPQIA
                     VAVIVEDGGNAGDEAGGGRTAGPIAKAVMEAVITR"
     gene            2928..4388
                     /locus_tag="PLK64_13235"
     CDS             2928..4388
                     /locus_tag="PLK64_13235"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPV80753.1"
                     /translation="MSPMTPGLGDVLGGRYTLTDRIAAGGMGDVWAATDRVLGRTVAI
                     KIMRPNTTDEGNFAARFRDEARHTAALSHPNIAAVYDYGEDDGTAYLVMELVPGLPLS
                     SMIAEGPMPPEQVRLILGQCALALAAAHEAGVVHRDVKPANVMVTPEGKVKLTDFGIA
                     RAVDAVGHTRTGEVMGTPQYLAPEQAMGQRVTGATDLYSLGVVGHEMLTGKRPFDAGS
                     AVATALAQISEPPPPLPAHVTDPLRSAIEACLAKDPAQRPSSAASLAALLGMPVSGVA
                     HASLATSVIPLSDLGATQPLATPTPLPTQVYAHPVAPYGHYPATAAGPAYPPQPQPPD
                     RRRGMGWWWLPVIAAFLVAGLFIWQNLVVGRANTPIPSVTVTRTVPRTSTASTPATTS
                     AAPTTSTAPTTTPVKTVTLVESDYVGQTTGVVRADLTTLGFLDIATKSVPSDRPKGEV
                     LGISPTGPVPVTTTIVITVSAGAAPTASSTAGGTSG"
     gene            4410..>5027
                     /locus_tag="PLK64_13240"
     CDS             4410..>5027
                     /locus_tag="PLK64_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPV80754.1"
                     /translation="MNTRRLLGGRYEVGEVIGRGGMADVHAGFDTRLSRQVAIKLLRS
                     DLARDAVFLARFRREAQSAAGLNHPNIVAIFDSGEDDSTDPTTGVAVHFPYIVMEVVD
                     GRTLREHLTATGPMPAAEAGRIVSDVLAALDYSHSKGIVHRDIKPANVMLGRGGAIKV
                     MDFGIARALADSAATMTQTQSVVGTAQYFSPEQARPLLQNLETETM"
BASE COUNT          740 a         1956 c         1570 g          761 t
ORIGIN      
        1 gacgaagtcg cggcgcaaca ccgagttcct gctgctgctc ttcgcggtcg cgatcgtcct
       61 cttcgcctgg gtcgacacgg aggtcgccaa ccgcggggcc gtccctgccg ggctagtggc
      121 catcggcggc ggcctgctgg tcctcgtcct gctcttccac ctggtgctgc ggtggaaggc
      181 gtcgtatgcc gacccgctgc tgctgcccat cgcgaccctg ctcaacgggc tcggcctggt
      241 gatgatccac cgcctcgacc tcgcggcagg tgactacggc ttcgacggcg acgcgttccg
      301 gcaactcatc tggaccggcg tctcgatcat cgtggcgggc gcgctgctct ggaccctgcg
      361 cgaccaccgg accctgcgcc gctacaccta caccgcgatg gccctcggtg tcctgctgct
      421 cgtcctgccc ctcgtgccgg ggttgggctc ggagcaactc ggctcacgga tctggatccg
      481 ggtcggtccg atgtccttcc agcctgggga gattgcgaag atcgctctcg cggtgttctt
      541 cgctggctac ctcgtgcagg cccgcgatgt cctctccctg gccgggcgca aggtccttgg
      601 cttcaccttc ccgcgcgggc gtgacctggg cccgatcatg gtcgcctggc tggtgagcgt
      661 cgccgtcctc gtcttcgaga aggacctggg ctcgtcgctg ctcttcttcg gcctcttcgt
      721 cgcgatgctc tacgtcgcca ccgagcgggt ctcctggatc gccatcggcc tcatgctgtt
      781 cgcggccggc gcctacgcgg catacctgct cttcccgcac ctgcagcggc gggtgatgct
      841 ctggctggac ccgttctcgt ccgactccct cgcccagtcc gaccaactgg ccaagggcct
      901 catggggatg gccgcgggcg ggctgttcgg caccggtctg ggcaagggac ggccgtggct
      961 ggtcgccttc gccgagagcg acttcatcgt cccctcgctg gccgaggagc tcgggctaac
     1021 cgggctgttc gcgatcctgc tgctctacgg cctgttcgtc cagcgcggtc tgcgcacggc
     1081 cctgggggtg cgtgacggct tcggcaagtt gctcgccctg gggctggcgt tctcggtcgc
     1141 actgcaggtc ttcgtcgtcc tcggtggcgt cacgcgggtc atcccgctca ccggactgac
     1201 gacgccgttt atgtcgctcg gcgggtcggc cctgctggcc aactggtcga tcaccgcgtt
     1261 gctgctgcgg atctccgacc aggcccgacg tcccgtcctg gacagcgacg gcctgcacac
     1321 catcccgcgg cgcaagccca cacccgaagc tgtagcggcg cccgcagcca caccggcagc
     1381 ctcgcccgcg ctcgcgccag cagtcgcaac cgggcccgca gcggcatccg cacccccagc
     1441 cgtcgctgcg ccgaccgagg cggtgccacg accgtgaaca cccccatcct gcggatgtcc
     1501 cgcctcgtcg gcgccctctt cgccatcctg ctcatctcgg cgacgatcat ccagttcgtc
     1561 caagccccct cgttgcgagc gcggccggac aaccggcgca ccttgctgga caactactcc
     1621 cgcgaccgtg ggtcgatcct cgtcggggac accgcgatcg cgacctcgac gccgtccgag
     1681 ggcgagctgg cctacctgcg cacctacccc cagggaccgc tctactccca cgtcaccggc
     1741 tactactcct acgtctacgg cgcgggcggt ggcttggagg gcagctccga cggattgcta
     1801 tccggatcgt cggacgcgct gttctacaag cggatcgccg acctgctcac cggcaagcaa
     1861 cccaccggcg ccagcctgga gctcaccctc aacgccaagg cccaggaggc cgccagcaag
     1921 gccctgggca accagcgcgg tgcggtcgtc gccctcgacc cccgcaccgg cgccatcctc
     1981 gccctcgtca gccaccccga atacgacccc aacacgctgg ccaaccacga ctccaacgcc
     2041 cagaagactg cctgggacgg catgctcgac gaccggaccc gccccctggt cgaccgaacc
     2101 atcagcggca acctctaccc gcccggctca gtgttcaaga tcgtcaccac cgcagccgcg
     2161 ctgtccacgg gggccgtcaa cgagacgacg cagatccccg gcccggcggt catggatttg
     2221 ccgcagacca ccgtcggcct gcccaaccac gacggcaagt cgtgcggccc tggcgacctc
     2281 accacgttgc agcacgccct cgagatctcc tgcaacaccg ccttcggatg gatcggcctc
     2341 aaggtcggcg gcgacgcgct gcgggaccag gccgccaagt tcggtttcgg tgagagcctg
     2401 cggatcccca tgcgagtcac tcccagcgtc ttccccagct ccctcaaccc gccccagacc
     2461 gctcagtcgg ccatcgggca gttcgacgtg cgagtcaccc ccctggaggt cgccatggtg
     2521 agcgcagcca tcgccaaccg cggcgtcgtc atgcgcccct acctcgtcca gcaggtggtc
     2581 ggcgccaacc tcgagatcct cgacactcac acccccgaac agatgtcggt ggccgtcacg
     2641 cccgagatcg ccgccatcat ctcgcggatg atggtgggcg tcgtcgacca gggctccggc
     2701 aagccggccc gcatcaacgg cgtctcggtc gccggcaaga ccggcaccgc cgaacagggc
     2761 aacgggctcc cggcgcacgc gtggttcacg tcgttcgcgc ccgccgacaa cccccagatc
     2821 gccgtcgcgg tcatcgtcga ggatggcggc aacgcgggcg acgaggccgg cggcgggcgc
     2881 acggccgggc ccatcgccaa ggccgtcatg gaagcggtga tcacccgatg agcccgatga
     2941 ctccaggcct gggcgacgtc ctcggcggcc gctacacctt gaccgaccgg atcgccgccg
     3001 gcgggatggg cgacgtctgg gccgccaccg atcgcgtcct gggccgcacc gtcgccatca
     3061 agatcatgcg ccccaacacc accgacgaag gcaacttcgc cgcgcgcttc cgggacgagg
     3121 cacggcatac cgcggcgttg tcccacccca acatcgcggc ggtctacgac tacggcgaag
     3181 acgacggcac ggcatacctc gtcatggagt tggtccccgg gctgccgctg tcgagcatga
     3241 tcgccgaggg gccgatgccc cccgagcagg tccgcctgat cctcggccaa tgcgcgctcg
     3301 ctctcgctgc cgcgcacgag gccggcgtcg tgcaccgcga cgtcaagccg gccaacgtca
     3361 tggtcacgcc cgagggcaag gtcaagctca ccgacttcgg catcgcccgc gccgtcgacg
     3421 ccgtggggca cacccgcacc ggcgaggtca tggggacgcc gcaatacctc gcgcccgagc
     3481 aggccatggg gcaacgggtg accggcgcca ccgacctcta ctccctcggg gtcgtcggcc
     3541 acgaaatgct cacggggaag cgcccgttcg acgccgggtc ggccgtcgcc accgccctcg
     3601 cgcagatcag cgagccgccc ccaccgctgc cggcgcacgt caccgacccg ctgcggtcgg
     3661 ccatcgaggc ctgcctcgcc aaggaccctg cccaacgccc ctccagcgcc gcctcgctcg
     3721 ccgccctgct cggtatgccg gtcagcgggg tcgcgcacgc aagcctggcg accagcgtca
     3781 tcccgttgtc agacctgggc gccacccagc cgttggccac tcccactccg ctgccgaccc
     3841 aggtctacgc ccatccggtg gcgccctacg ggcactatcc agcgacggca gcggggccgg
     3901 cctacccgcc gcagccgcag ccacccgacc gccggcgcgg gatgggctgg tggtggctgc
     3961 cggtgatcgc cgccttcctc gtcgccggcc tgttcatctg gcagaacctc gtcgtcggcc
     4021 gggccaacac gccgatcccc tccgtgacgg tgacgaggac cgtcccccgg acgagcacgg
     4081 ccagcacgcc cgcgacgacc agcgccgccc cgacgaccag caccgccccg acgaccactc
     4141 cggtcaagac ggtgaccctc gtcgagtccg actacgtcgg tcagacgacg ggagtcgtgc
     4201 gggccgacct caccaccttg ggcttcctgg acatcgccac gaagtccgtt ccctccgacc
     4261 gtcccaaggg cgaggtcctc gggatcagtc cgaccggccc ggtgccggtc accaccacca
     4321 tcgtcatcac ggtgtccgcg ggagcggcac cgacagccag cagcaccgca ggaggcacca
     4381 gtggctagcg cactgcacgg gggatccgca tgaacactcg acgcctactc ggggggcgtt
     4441 acgaagtggg cgaggtcatc ggacgtggcg gcatggccga tgtccacgcg ggcttcgaca
     4501 cgcgcctgtc ccgacaggtg gcgatcaaac tgctgcgctc cgacctggcg cgcgatgccg
     4561 tcttcctcgc ccgcttccgc cgtgaggcgc agtcggcggc cgggctcaac caccccaaca
     4621 tcgtcgccat cttcgactcg ggggaggacg acagcaccga tcccacgacc ggggtcgcgg
     4681 tgcacttccc ctacatcgtc atggaggtcg tcgacggccg caccctgcgt gagcacctga
     4741 ctgccaccgg cccgatgccc gccgccgagg ccggacgcat cgtgtcggac gtcctcgccg
     4801 cgttggacta cagccactcc aagggcatcg tccaccggga catcaagccc gccaacgtca
     4861 tgctcggtcg cggcggcgcg atcaaggtca tggacttcgg gatcgcccga gccctcgccg
     4921 acagcgcagc gacgatgacc cagactcagt cggtcgtcgg caccgcgcag tacttctcgc
     4981 ccgagcaggc ccgcccattg ctgcagaatc tggagacaga aactatg
//
LOCUS       DAOMZY010000265         5017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_15727_length_5017_cov_4.461911, whole genome shotgun sequence.
ACCESSION   DAOMZY010000265 DAOMZY010000000
VERSION     DAOMZY010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15727_length_5017_cov_4.461911"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..946)
                     /locus_tag="PLK64_13245"
     CDS             complement(74..946)
                     /locus_tag="PLK64_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005270205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPV80755.1"
                     /translation="MSINVADLVAVDVHVHVEVDDHGHTALPQSFLDWSAKYFKTGGP
                     RTPTIDEIAARYRERKIAAVIFTVDVEKSMGQPRIPNEVVAQGAARNADILIPFASID
                     PARGAEGVKELERLIADHGVKGLKFHPSLQNFAPNDGSAYGLFEVLAAHKLPAVFHTG
                     QTGIGAGMPGGGGVKLRYSDPMLLDDVAADFPDLPVVLAHPSVPWQDQAISMATHKGN
                     VYIDLSGWSPKYFPPALVKAANGALRGKVLFASDFPVIDTDRWLADFETLDIKPEVRP
                     MILKDNAVRLLGLA"
     gene            complement(981..1889)
                     /locus_tag="PLK64_13250"
     CDS             complement(981..1889)
                     /locus_tag="PLK64_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPV80756.1"
                     /translation="MDLSGKVAIVTGSGRGLGLAYAKALAGAGAAVVVNDIDEAVAQA
                     AAAEITAAGGKAIAVAAAVGSSESAQALVDAAVETFGRLDVLVANAGVLRDKVVWKMT
                     DEDFDLVTQVHLKGTFTCARAAIVRMKEQGEGGRLILIGSPAGQYGNFGQTNYAAAKA
                     GIVTFAKVWAMELSRDKITANAVVPVAATAMTATVPFLKPYVDAMAAGEPMPEFARRE
                     LAFGTPEDVAGLVVYLASDDSAGVNGQAIGIGGDRLSLYSHPDQIVKEYAAGGWSADA
                     IADVWPSRFAPSQQSVGETLPTPPSA"
     gene            2030..2461
                     /locus_tag="PLK64_13255"
     CDS             2030..2461
                     /locus_tag="PLK64_13255"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPV80757.1"
                     /translation="MTGDQGTSIREDVVVLLARASGSALRTANAALEPFGFRARHYAT
                     VKIAAEGGGVPQRQIGAVLGLDPSAVVALVDDLESLGLVQRQPDPDDRRTRLVSPTPA
                     GLTMLEKVSPVVGRVQERSMGGLTAAERDTFVRLLRRVVNE"
     gene            complement(2484..2942)
                     /locus_tag="PLK64_13260"
     CDS             complement(2484..2942)
                     /locus_tag="PLK64_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HPV80758.1"
                     /translation="MTDSTPVAQDPPSVTPWVTLEQVNAMAAGTLHERMGVVFTEIST
                     SRAVATMPVAGNTQPYGLLHGGASVVLAEGVGSFLAVVLAGPGRAAVGVDINATHHRP
                     VMSGTVTAVATPIAVGRTTASVEIVITDDEGRRTCTCRFTAQLRDAPPSR"
     gene            2941..>5017
                     /gene="polA"
                     /locus_tag="PLK64_13265"
     CDS             2941..>5017
                     /gene="polA"
                     /locus_tag="PLK64_13265"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPV80759.1"
                     /translation="MGSRLPYVSDTPRLLLLDGHSLAYRAFYALPAENFSTHTGQHTN
                     AVYGFTSMLINVLRDEAPTHIAVAFDVSRQTFRTEAYAEYKAGRSATPREFEGQISLV
                     QEILGALRIPFVQVEGYEADDVIATLTTMATDEGMDVLICSGDRDAFQLVTDHVTVLY
                     PVRGVSELWRMTPAAVTEKYGVPPTRYSDLAALVGESSDNLPGVPGVGPKTAAKWIGM
                     YAGLDGIVANIDQIGGKAGESLREHLDSVLRNRRLNQLVHDLQVGVELGDLARHSWDR
                     EQVHEVFDALEFRVLRERLFATFEAPEPEADSGFALDGQVLGSAEVTGWLAQHAPAGV
                     RTGLQVLGRWGRGTGDARGLALATGAGAAAYVDLAGLDDVGERALAHWLADPTRPKAI
                     HDGKGPAAALAARGLDLAGVTSDTALAAYLVKPDQRDYDLADLAVRLLRRELRDDTTD
                     TGQGMLDFGEGDNEANEAMVTARAVVDLADALDVELERIGGSALLHEVELPLVGVLGR
                     MERAGIAVDIEALRDLEADFAAKVREAQEDAWEAIGRRDVNLGSPKQLQEVLFDQLGL
                     PKTKRTKTGYTTDADALTELYAKAEHPFLEALLRHRDAAKLRVTVEGLIKSVDDDGRI
                     HTTYLQTVAATGRLSSTDPNLQNIPIRTEEGRRIREMFVVGSGFESLMSADYSQIEMR
                     VMAHLSGDDG"
BASE COUNT          764 a         1645 c         1824 g          784 t
ORIGIN      
        1 ggcgccgagc gggacgccga tcagggaggc caagtcgtcg aagtgcaccg tagtcgtcat
       61 cgtgagctcc ttttcaggcc aggccgagca gccggaccgc gttgtccttg aggatcatcg
      121 gtcggacctc gggcttgatg tcgagcgtct caaagtcggc cagccagcga tcggtgtcga
      181 tgaccgggaa gtccgaggcg aagagcacct tgccacgcag cgcgccgttg gcggccttga
      241 cgagggccgg cgggaagtac ttcggcgacc agccggacag gtcgatgtag acgttgccct
      301 tgtgggtcgc catcgagatc gcctggtcct gccacggcac cgaggggtgc gcgaggacga
      361 ccgggaggtc ggggaagtca gccgcgacgt cgtccaggag catcgggtcg ctgtagcgca
      421 acttcacgcc gccaccaccc ggcatcccgg cgccgatccc ggtctgcccg gtgtggaaca
      481 cggcgggcag tttgtgcgcc gcgagcacct cgaacagccc ataggcgctg ccgtcgttgg
      541 gggcgaagtt ctgcaggctg gggtggaact tgaggccctt gaccccgtgg tcggcgatca
      601 ggcgctcgag ttccttgacc ccctcggccc cgcgcgcggg gtcgatgctg gcgaacggga
      661 tgaggatgtc ggcgttgcgc gcggcgccct gcgcaaccac ctcgttgggg atgcgcggct
      721 ggcccatcga cttctcgacg tcgacggtga agatcacggc cgcgatcttg cgctcgcggt
      781 aacgggccgc gatctcgtcg atcgtcgggg tgcgggggcc accggtcttg aagtacttcg
      841 ccgaccagtc gaggaaggac tgcggcaagg ccgtgtgtcc gtggtcgtcg acctcgacgt
      901 gcacgtgcac gtcgacggcg accaggtcgg ccacgttgat gctcatcagg aactccttgg
      961 tatgccgtgt ggctgggtga tcaggccgag gggggagtcg gaagcgtctc gccgacggac
     1021 tgctggctgg gcgcgaacct cgagggccag acgtcggcga tggcgtcggc gctccagccg
     1081 ccggcggcat actccttgac gatctggtcg gggtgggagt agagcgaaag gcggtcgccg
     1141 ccaatgccga tggcctggcc gttgacccct gccgagtcgt ccgaggcgag gtagacgacc
     1201 aagccggcga cgtcctcggg agtgccgaag gccagttcgc ggcgagcgaa ctcgggcatc
     1261 ggctcgccgg ccgccatggc gtcgacgtag ggcttgagga agggcacggt ggcggtcatc
     1321 gccgtggccg cgaccgggac gacggcgttg gccgtgatct tgtcccggga gagttccatg
     1381 gcccacacct tggcgaaggt gacgatgccg gccttggcgg cggcgtagtt cgtctggccg
     1441 aagttgccgt actggcccgc gggggagccg atgaggatga gtcgcccgcc ctcaccctgc
     1501 tccttcatcc gcacgatcgc ggcgcgggca caggtgaagg tgcccttgag gtggacctgg
     1561 gtgacgaggt cgaagtcctc gtcggtcatc ttccagacga ccttgtcacg cagcacgccg
     1621 gcgttggcga ccaggacgtc caggcggccg aaggtctcga cggccgcgtc gacgagggcc
     1681 tgggcggatt cggacgagcc gacggcggcg gcgacggcga tggccttgcc cccggctgcg
     1741 gtgatctcgg cggccgcggc ctgagcgaca gcctcgtcga tgtcgttgac gacgacggcc
     1801 gcgcctgcgc cggccagggc cttcgcgtag gcgagcccga gccccctgcc gcttcccgtg
     1861 acgatcgcga ccttgccgga caggtccatt gctgctcctt cgtggcgtgg gcattgcgtg
     1921 gggggcatgg agagccgggc gtcggcgccg cgccccgagg tatgtataag tgacaacgat
     1981 tgtgaaagtc aatcattgaa gattgtgacc tgcgtgccac aatgggggca tgacaggcga
     2041 tcagggcacg agcatccgtg aggacgtcgt ggtcctgttg gcgcgggcgt ccggttcggc
     2101 gttgcgcacc gcgaacgcgg ccctggaacc cttcggtttc cgggctcggc actacgcgac
     2161 ggtgaagatc gccgccgagg gtggcggggt cccgcagcgg cagatcggcg cagtcctcgg
     2221 cctggacccc agcgccgtcg tggccctcgt cgacgacctc gaatcgctcg gtctggtcca
     2281 gcggcaaccc gacccggacg atcggcgcac ccggctggtg tcgccgacgc cagcgggcct
     2341 gaccatgctc gagaaggtct ccccggtcgt ggggcgcgtg caggagcgtt cgatgggtgg
     2401 cctgacggcc gccgagcggg acaccttcgt gcggctgctg cgccgcgtcg tcaacgagta
     2461 gtcgtccgca cgcagccgtg acgtcagcgg ctcgggggag cgtcgcgcag ttgcgcagtg
     2521 aaccgacagg tgcaggtacg ccggccttcg tcgtcggtga tgacgatctc gaccgacgcc
     2581 gtcgtgcgtc ccacggcgat cggcgtggcc accgcggtga ccgtgcccga catcaccggc
     2641 cgatggtggg tggcgttgat gtcgaccccg accgcggccc gacccggccc ggccagcacg
     2701 accgcgagga acgaccccac cccttcggcg aggacgaccg aggcaccgcc gtgcagcaga
     2761 ccgtagggct gggtgtttcc tgcaacgggc atcgtcgcca cggcgcggga cgtggagatc
     2821 tcggtgaaca ccacccccat gcgctcgtgc agggttccgg cggccatcgc gttgacctgc
     2881 tcgagcgtga cccacggggt gactgagggc ggatcctgcg cgacgggggt gctgtcggtc
     2941 atgggctcta ggctgccata cgtgtcggac acgcctcgcc tgctgctctt ggacggtcat
     3001 tcgttggcct atcgcgcgtt ctacgccttg cccgcggaga acttctcgac ccacaccggg
     3061 cagcacacca acgccgtcta cggcttcacc tcgatgctca tcaacgtgct gcgtgacgag
     3121 gcgccgaccc acatcgcggt ggctttcgac gtgtctcgcc agaccttccg gaccgaggcc
     3181 tacgccgaat acaaggccgg ccggtcggcg acgccgaggg agttcgaggg ccagatctcc
     3241 ctcgtacaag agatcctcgg ggcgctgcgc atcccctttg tccaggtcga gggttacgaa
     3301 gccgacgacg tcatcgccac cctcacgacg atggcgaccg acgagggcat ggacgtcctc
     3361 atctgttccg gtgaccgaga cgccttccaa ctcgttaccg accatgtcac cgtcctctac
     3421 ccggtccgcg gggtgtcaga gctgtggcgg atgacccccg cggcggtgac cgagaagtac
     3481 ggtgttccgc ccacgcgcta cagcgacctt gccgcgctgg tcggggagtc ctccgacaac
     3541 ctcccgggcg tccccggggt cggccccaag accgcggcca aatggatcgg gatgtatgcc
     3601 ggcctcgacg gcatcgtcgc gaacatcgac cagatcggcg gcaaggcggg ggagtcgctg
     3661 cgcgagcatc tggactcggt gctgcgcaat cgccgcctca accagctggt gcacgacctg
     3721 caggtcggcg tggagctggg cgacctggcc cgccactcct gggaccgcga acaggtgcac
     3781 gaggtcttcg atgcgttgga gttccgcgtg ctgcgcgagc ggctgttcgc gaccttcgag
     3841 gcccccgagc cggaagccga ttccgggttc gcccttgacg ggcaggtgct gggctcggcc
     3901 gaggtgaccg ggtggctggc gcagcacgcc cccgcggggg tccgcaccgg gctgcaggtg
     3961 cttggccgct ggggccgggg caccggcgac gcgcgcgggt tggcgctggc caccggagcc
     4021 ggcgcggcgg catacgtgga tctcgccggg ctcgacgacg tgggggagcg cgcgctcgcg
     4081 cactggctgg ccgaccccac ccgcccgaaa gcgatccacg acggcaaggg gcccgccgcg
     4141 gcgctggcgg cgcgggggct ggacctcgct ggggtgacga gcgacacggc gctggcggca
     4201 tacctcgtca aaccagacca acgcgactac gacctggccg acctcgccgt gcggctgctg
     4261 cggcgggaac tgcgcgatga cacgaccgac accgggcagg gcatgctcga cttcggcgag
     4321 ggggacaacg aggcgaacga ggctatggtg accgcccgcg cggtcgtcga cctcgccgac
     4381 gcgctcgacg tggagctgga gcgcatcggc ggctcggcgc tgttgcacga ggtcgagttg
     4441 ccccttgtcg gggtgctcgg ccgaatggag cgggccggga tcgccgtcga catcgaggcg
     4501 ttgcgcgatc tggaggcaga tttcgccgcc aaggtgcgtg aggcgcagga ggatgcctgg
     4561 gaggcgatcg ggcgacgcga cgtcaacctc ggtagcccga agcaactgca ggaggtgctc
     4621 ttcgatcagc tcgggctgcc caagaccaag cgcaccaaga ccggctacac cacagacgca
     4681 gacgccctca ccgagctgta tgccaaggcc gagcatccct tcctcgaggc gttgctgcgc
     4741 catcgtgatg cggccaagct gcgggtcacg gtcgaggggc tgatcaagag tgtcgatgac
     4801 gacggacgga tccacacgac ctacctgcag accgttgccg cgactggtcg gctgagttcg
     4861 accgacccca acctgcagaa catcccgatc cgcaccgagg agggtcggcg gatccgggag
     4921 atgttcgtgg tcgggtccgg gttcgagtcc ctcatgtcgg cggactacag ccagatcgag
     4981 atgcgggtca tggcccacct gtccggcgac gacgggc
//
LOCUS       DAOMZY010000266         5011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_15755_length_5011_cov_7.629944, whole genome shotgun sequence.
ACCESSION   DAOMZY010000266 DAOMZY010000000
VERSION     DAOMZY010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15755_length_5011_cov_7.629944"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            328..594
                     /locus_tag="PLK64_13270"
     CDS             328..594
                     /locus_tag="PLK64_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HPV80760.1"
                     /translation="MAVEPLDEVRNHLSDVIDRVEREHERVTVTRNGRAVAVILSPDD
                     LTELEESLAVLSDPQALADIREADAAFAAGDVACGVDAVRALRP"
     gene            complement(610..1155)
                     /locus_tag="PLK64_13275"
     CDS             complement(610..1155)
                     /locus_tag="PLK64_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80761.1"
                     /translation="MSFDLDADQIRELLDELDRRLRTDGVAATLYIIGGAAVALQLPD
                     ADRRTQDIDGLTTNDKVAGVVSAMAAELGLPENWLNGAAAPFVPAPPRGAMDPPTSAG
                     LHVELAPLRHLLAMKLAAGRARDRADITAIAAALGIDADTAVQLTLETYGSDALAVFT
                     NAEDVRLEADAAIPQRGAGGH"
     gene            complement(1152..1709)
                     /locus_tag="PLK64_13280"
     CDS             complement(1152..1709)
                     /locus_tag="PLK64_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPV80762.1"
                     /translation="MATTEQLRARLRALIDAAGLNGSQAAFAIGRTPSRLGDYLAGRT
                     VPSAMVLLDLEEAAEQASRRTWMRAADVVDAVAEHADSDPVWAMRMLLQGRDQSLALS
                     TPARQAIWATGSPSRRLAGPWKTLTYQLLRDSARDGRPIPRWLVAATPLEQPWAPLPV
                     REDRPLHEGLAKVGVRVNERELLTA"
     gene            2327..2557
                     /locus_tag="PLK64_13285"
     CDS             2327..2557
                     /locus_tag="PLK64_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80763.1"
                     /translation="MEVVDRPVEVVRTITKVQRVIVEAPIAVAPQTVGEWSDFLAELT
                     DRLDRGRIYDRDVPALRPAVAALVDAFNRRWA"
     gene            2905..3966
                     /locus_tag="PLK64_13290"
     CDS             2905..3966
                     /locus_tag="PLK64_13290"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HPV80764.1"
                     /translation="MNSLTDLREVVDVVIGVDTHVHTHSAAVVDAATGGVLGEITVEA
                     TSDGYGELVEFANQHATLRAWAIEGTGGHGAGLSRHLMELSEIVIEVDRPKRAARRNG
                     AKSDPLDAIRAAREAMARPRLGTPRTGGERQALSVLLAARRSAVNASTEAQLQVFSLV
                     IAAPEPIRVRFRGQKLAAMLNTAANLRVHASWDTETTSTVVALRTLARRAHAMLKEAR
                     ELQKAMVAIIRSWRPDLLEEFGVGPIVAATVLCAWSHPGRIHSEAAFAMLAGVAPIPA
                     NSGQVTTRYRLNRYGDRQLNNALHTVVASRIQYQPATRDYVARRTAQGKTGREIKRCL
                     ARYVARDLYRLLESGAPAA"
     gene            4421..>5011
                     /locus_tag="PLK64_13295"
     CDS             4421..>5011
                     /locus_tag="PLK64_13295"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80765.1"
                     /translation="MVTAVELEHTEIRNRLINTERLRVEDIWMMRGEIPGDHINWTVF
                     LARAGMGNATSAALVGLAARNLEANLVAFVGSAGGLKPNDQRHFDVVVASRIHNPYAG
                     KQVPADAESKLLGRDKTYVVPAPLVAVVNACIEDCEWTTSTRSQHYNANHPHAFVAPI
                     VSVDAVQADTRGPVLLEILNRFQDAAALDMESFGLAA"
BASE COUNT          872 a         1752 c         1587 g          800 t
ORIGIN      
        1 ggcgaaggga ctcgaaccct caaccccctg tttacaagga gtgtgggatt tcgtttctcc
       61 ccgcccctga cctgcgacga tgccaaattc cggcgtcgga agtggcctcg tgcgacagcc
      121 gtgcgacaga gatgtccatc tatgtacacc tgtgtcgtgc gtcagcgtcc aggtcctctg
      181 ggaacgcagc aggaactttc atgcgccccg ctcggctcct ccttcgacga tacgggacca
      241 gtcaggcggg cccaaggcgt tgtgcacaga cggacgacct ggcttctggc cgcagggcga
      301 atcgccgcgg acgccacact ggtgtccatg gccgtcgaac cgcttgacga agtacgcaac
      361 cacctcagcg acgtcatcga ccgcgtcgag cgcgagcacg agcgagtgac ggtcacccgc
      421 aacgggcgcg cggtggccgt catcctcagc cccgacgacc tcaccgagct cgaagagtcg
      481 ctggcggtcc tcagcgaccc gcaagccctg gccgacatcc gtgaagcgga tgccgcgttc
      541 gccgccggtg atgtcgcctg cggagtcgat gccgtccgtg ctctgcgtcc gtgagcaacg
      601 gccctcgcct cagtgccctc cagcgccacg ttgtgggatg gccgcatcgg cttccagccg
      661 cacgtcctcg gcgttggtga agactgccag cgcgtcgctt ccataggtct cgagcgtcag
      721 ctgcacggca gtgtcagcgt caatgccgag cgcggcggcg atggccgtga tgtccgctcg
      781 atctcgggca cgtccggctg ccagtttcat ggccaagagg tgcctcaagg gggccagctc
      841 cacgtgcagc ccagcggaag ttggggggtc catggcgcca cgcgggggcg ccgggacaaa
      901 cggcgcagcc gccccgttca accagttctc cggaaggccc aactcggccg ccatcgcgct
      961 gaccacgccc gcgactttgt cattagtcgt gagtccgtcg atgtcctgcg tgcgccgatc
     1021 agcatcgggc agctgcaggg ctaccgccgc gcccccgatg atgtagaggg tggccgcgac
     1081 accgtctgtg cgcagccggc ggtccaactc atcgaggagt tcgcggatct gatccgcatc
     1141 gaggtcgaaa ctcatgcggt caggagctca cgctcgttga ccctgacgcc aaccttggcc
     1201 agaccctcgt gcagcggccg gtcctctcga accggcaagg gtgcccacgg ctgctccaaa
     1261 ggagtcgcgg cgacgagcca cctggggatc ggtcgcccgt cacgcgcact gtccctcagc
     1321 aactggtagg tgagcgtctt ccacgggccg gccagccgac ggctcgggga tcccgtggcc
     1381 cagatggcct gcctggccgg cgtgctgagc gcgagtgact ggtctcggcc ctggagcagc
     1441 atccgcatag cccagaccgg gtcactgtcg gcatgctccg caacggcgtc gaccacgtcg
     1501 gccgcgcgca tccacgtgcg cctggacgcc tgctctgcgg cttcctcgag atcgaggaga
     1561 accatcgccg aggggaccgt gcgcccggcg aggtagtcgc cgagccggga cggggtacgc
     1621 ccgatcgcga acgcggcctg gctgccgttc aaacccgcgg cgtcgatcag cgcgcgaagg
     1681 cgcgcacgaa gctgctcggt cgtggccatg ccccaagaat acccatattc ggttacttat
     1741 cgaatcgaac tcgtccttac cacatccgca gcccccgccc tcccgccaac aggccgccgt
     1801 cccgcactcc ccgcgaggca acaccccagc cgacaccccc cgagcctccc cgaacggcct
     1861 tcgttcctac ccgacgaatc cgcacgatgt cgtggcccgc acaccccatg cattgtcggc
     1921 cggcatccca gtcgttgtga cgactcgccg atccgagacc gccccagaaa tgtggccccc
     1981 aaggggcctc ccgaatgcgt agggaagtaa ggacaccaac caggaggcga cgatggaacg
     2041 gaaactcggg aagcaggcga agcggcaggc ggcacgccta gccatgctcg acggaatccc
     2101 cgcgaaccgg aaggatcctc gccgtcgagc cgcgcagcag gtacccacca cggatcccaa
     2161 ggccgcgcgc cggctgccgc gcgctccggg gggcacggtg tcctgtggct ggtgcggcgc
     2221 aaccgtcacc gtgcccaacc gcggccggat ccccaagtgg tgctcagcca cctgccgtca
     2281 ccgcgcctgg gagcagcgtc gggccgccgc ctcaggacgc gcagccatgg aggtcgtcga
     2341 ccgaccagtg gaggttgtac ggacgatcac gaaggtgcag agggtcatcg tcgaggcgcc
     2401 catcgctgtc gcaccgcaga cggtgggcga gtggtccgac tttctcgcgg agctcaccga
     2461 ccggctcgac cgcggtcgca tctacgaccg cgacgtgcca gccctccggc cggccgtcgc
     2521 cgctctagtc gacgccttca accggcgttg ggcctgatgc ccgtccgaca cggccatccg
     2581 cctttcacca aacgaggtca gggccctcgt cagtagggct gacaggtggt cgattcgcgg
     2641 cgctgagact gtgaggatcg ccaacgcagt ctaaaggtgc tgggctgtgg ttggtgacca
     2701 cgggtgtgac ccccgctgag actggcttcg tgccttggtg cgacttgtcc gctcgcggtc
     2761 accggccccg gcccggttgg aggagctggc ctgatcagga gcttgaccac ctggcgttaa
     2821 cggcgccggg cgtgtctcat cacagtcctg ccgagcctcc atcccggacc ggaaccacgt
     2881 cccgtccgga gaaggagaga acgcatgaac agtctgaccg atctgcgtga ggtggtcgat
     2941 gtcgtcatcg gcgtcgacac ccacgtccat acccactccg cggccgtggt cgacgctgcc
     3001 acgggtggtg tcctgggcga gatcaccgtc gaggcgacaa gcgacggcta cggcgagctg
     3061 gtggagttcg ccaaccagca tgcgacgttg cgggcctggg cgatcgaggg gaccggtggt
     3121 cacggcgccg gtctgagccg tcacctgatg gaactgtcgg agatcgtcat cgaggtggac
     3181 cgaccgaagc gggctgcgcg tcgcaacggc gcgaagtccg atccgctcga tgcgatccgc
     3241 gctgcgcgtg aggcgatggc ccggccgcgg ctgggaaccc cccgcaccgg gggcgaacgc
     3301 caagcactgt cagtgctttt ggctgctcgc cgctccgcgg tcaatgcctc caccgaggcc
     3361 caactgcagg tgttcagcct cgtgatcgcc gcacccgaac ccatccgggt caggttccgc
     3421 ggccagaagc tagccgcgat gctcaacacc gcagcgaacc tgcgagtgca cgcttcctgg
     3481 gacaccgaga caaccagcac cgtggtggcg ctgaggacct tggcgcgtcg cgctcacgcg
     3541 atgctcaaag aggcacgcga actgcagaag gcgatggtgg ccatcatccg cagctggcgc
     3601 cctgacctgc tcgaggagtt cggcgtcgga ccaatcgtgg ccgccaccgt gttgtgcgcc
     3661 tggtcccacc ccggccggat ccactccgag gcggcgttcg cgatgctcgc cggcgtggca
     3721 ccgatcccag cgaacagcgg ccaagtcacc acccgctacc ggctcaaccg ttacggcgac
     3781 cgccagctca acaacgcact gcacaccgtg gtcgcgagcc ggatccagta ccagccagcc
     3841 acccgcgact acgtcgctcg ccgcactgcc caaggcaaga caggtcgaga gatcaaacgc
     3901 tgccttgccc gctacgtcgc ccgcgacctc taccgcctac ttgaaagcgg ggcaccagct
     3961 gcttgacaaa gcataggagc gtcgcgcctc gatccgcact gtcctgccga tgcgcggaga
     4021 acctgtcgcg cgtccttcgc gaagagtgac gctcccaatc gttcctcgac cggtgcagac
     4081 gtgttcacgg cgacctcgct aggtgcacaa gttcatgttc actagaggat gtctattgca
     4141 catgccgtgc gcaaaatgtc agactggttc catggccctc tcaccgcttc actcatcaat
     4201 cgttcggatg ctcaggcgtt cggcagcgcc gttgagcggt aggcaggttg ccaacgtcgt
     4261 aaatgtggct cccaacaccg cactcaaagc gctcaagagc ctgcaggtgg gcggcctagt
     4321 cgaatctcgc cgcgagggcc gagcgacgct gtggaccaca accgctgagg tgtccctact
     4381 cccagagctc agtgggccta cccagacgcg ggtagcactc gtggtcacgg ccgtcgaact
     4441 ggagcacact gagatccgca atcgcctaat caacacggag cggctgcggg tggaagacat
     4501 ctggatgatg cggggcgaga ttccaggcga tcacatcaac tggaccgtct tcctcgcccg
     4561 agcggggatg gggaacgcaa cctctgcggc cctcgtcggc cttgccgctc gcaaccttga
     4621 ggccaatcta gttgcgttcg tgggttcggc cggcggcctg aagcccaacg accagcgcca
     4681 ctttgacgtc gtggtggcga gccgcattca caacccctac gcgggcaagc aggtgccggc
     4741 cgatgccgag tcgaaactcc tgggccgaga caagacctac gtcgttcccg cacccctcgt
     4801 cgcggtggtg aacgcatgca tcgaggactg cgaatggacc acatcaacgc gcagccagca
     4861 ctacaacgcc aatcaccctc acgccttcgt ggctccgatt gtgtcggttg acgcagttca
     4921 ggctgacact cgcggtccgg tcctgctcga gattctcaac cgattccagg acgcggccgc
     4981 cctcgacatg gagtcattcg gcctcgccgc c
//
LOCUS       DAOMZY010000267         5008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_15777_length_5008_cov_6.457702, whole genome shotgun sequence.
ACCESSION   DAOMZY010000267 DAOMZY010000000
VERSION     DAOMZY010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15777_length_5008_cov_6.457702"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1551)
                     /locus_tag="PLK64_13300"
     CDS             complement(<1..1551)
                     /locus_tag="PLK64_13300"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPV80766.1"
                     /translation="MSDVRVMRARRFHTMEPGFPTAEAVAVRGDRILAVGSFDAVVAA
                     LGDAPFEVDDTLSDYVVLPGLMDQHLHPLTGAAAMSMEIASTEDWVLPGHTSPAAHTP
                     EEYLARLKAAERALTDTGDDGWLLTWGYHPLWHGPLDRTVLDQVSTTRPILVWHRSCH
                     EVYLNTTAIAQAGVTAEWLATQSGHATSQVDIAAGHSWEAGFMDLVITRVATYMLPRD
                     QLARGLHLLVAYLHQHGVTAINEPGLIMAVEPVDLYQEILGADDTPFTSTFLVDGRSQ
                     LSAGLDPSEVVANAEALIARAPEGKVRLLNRHVKLFADGAIISQRMQMLEPYIDEDGN
                     PDPSHHGEWIIEPEVLDRYYRAYWDAGWQVSTHVTGDLGLQVLLDVIERCMIATPRND
                     HRCVIVHFSNSTEAQVDRIARLGCLVSANPYYPVGFADRFGQHGVGPERADVMVRNES
                     VLARNIPLAFHSDLPMAPAEPLFLAWCAVTRRTETGRIAAPEQRISIDEALRAVTMGP
                     AYTWRTRAY"
     gene            complement(1681..4032)
                     /locus_tag="PLK64_13305"
     CDS             complement(1681..4032)
                     /locus_tag="PLK64_13305"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HPV80767.1"
                     /translation="MTRRGANPALAALASLAALLGLTTLVVTQTWYARTIWFVFVAVG
                     IGALIRRFVSRSGWLIVPAQVLGVLLMATWQYAGSTTWWGVPTWSTVGRFTELFTLFG
                     TTVAESSAPLLANVGVEVTLALIGAGLAILVDALAVTRAAPAAAGLPLLTAFLAAAAN
                     SGSALPPAYFIIAALAWLLMLGRQAQVGMRGWASAAARPMAPISGAALDSAPELRFGT
                     IARRLGAVGLVAAIALPAVLPHLPPRYLLDGLARSADGAGRGVGKVGFNSTLDVGLSL
                     TSGGTGTILTYRTNAPAAAPLRVLAATAYDGTNWSRPAPTLGRSARLDLADTVVRSER
                     TIVVESNALDPPSLATPQPILSADLSGVSWQVDVATSDVSVQTRPNAYSTTYLEPVLT
                     ADLLREGIDGRPGPDRLPTNRTMSAALAVDPRSEAAIRALTTTVTAGAATPYDEAVAI
                     QEWLRSKGGFTYSLTLPEAPLVSGVVGDPISAFLQTKTGYCVQFTSAMVMMSRAAGIP
                     ARVAIGFLPGSQDGGLWTVTAGDAHSWPELYFPGAGWVRFEPTPSVRTGTAPSWTVPP
                     VTPTPLATSTARSTEDPLDRPDRDAPDDADDTTTARTELDLPILDRVLSWVAQIPHLF
                     LLVVGLVVVATLVLPVTAALLRRVRSRRGGPPNEVIEAHWKLFTSELHDLGITTPAGG
                     TLRDSQAHVARAGYLGEPARQSLATVVDTVELARYARPGTSLAVDLEPLTRSVISDVA
                     RTRSWRHRARALLLPGDARRWWGRAVASLRSAPRAVRERIRRG"
     gene            complement(4029..>5008)
                     /locus_tag="PLK64_13310"
     CDS             complement(4029..>5008)
                     /locus_tag="PLK64_13310"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPV80768.1"
                     /translation="PRVLLGSLGSGERRALTYAVRPPLRGRHLLGPLTVQLRDPFGLS
                     QRFVEAGEPTEVLVLPRVYPLGGNRPPGTGVGAEGEIPFMVALHGEDDQSIREYRDGD
                     DLRRIHWPATARTGELMVRQEDRPARRRAIVLLDPRVDAHAGVGVGSSFEWAVSAAAS
                     MVVHLSGLGYAVHLLTPDTVGDGPDALPIDPAQALAVLAVAEPDSGLALARLTRAALG
                     LLSGGGLLVAVLAAHDESALRSVATLRHPGATALGIVLDTGSFAGRPAGGGANAGGGA
                     VASEAARAVDLLRGAGWRATTVTRDDTVEHAWSQVGSRRDAQVALGASR"
BASE COUNT          798 a         1682 c         1872 g          656 t
ORIGIN      
        1 gtaggcacgg gtccgccagg tgtaggcggg ccccatggtc acggcccgca gagcctcatc
       61 gatgctgatc cgctgctcgg gcgcggcaat ccggccggtc tcggtgcggc gcgtgaccgc
      121 gcaccacgcg aggaagagcg gttcggccgg cgccatcggc aggtcggagt ggaaggccag
      181 gggaatgttg cgggccagca ccgactcgtt acgcaccatg acatcggcgc gctcggggcc
      241 gaccccgtgt tgcccgaagc gatcagcgaa gccgacgggg tagtaggggt tggccgagac
      301 caggcagccg aggcgtgcga tccgatcgac ctgtgcctcg gtggaattgg agaagtgcac
      361 gatgacgcag cggtgatcat tgcgcggggt ggcgatcatg caacgctcga tcacgtcgag
      421 cagcacctgg agcccgagat ctccggtgac gtgggtgctg acctgccacc cggcgtccca
      481 gtaggcgcgg taataccggt cgagaacctc tggctcgatg atccattcgc cgtggtggct
      541 cggatctgga ttcccgtcct cgtcgatgta cggctcgagc atctgcatgc gttgggagat
      601 gatcgccccg tcggcgaaga gtttgacgtg acgattgagc aggcggacct tgccctcggg
      661 agcgcgagcg attaatgctt ccgcgttggc gacgacctcg gaggggtcca gcccggcgga
      721 cagttggctg cgtccgtcga ccaagaaggt ggaggtgaac ggcgtgtcgt cggcaccaag
      781 gatctcctgg tagaggtcga ctggctccac cgccatgatg agccccggct cgttgatcgc
      841 cgtcaccccg tgttggtgca gataggcgac caacaggtgc agtccgcggg cgagttggtc
      901 acgggggagc atgtaggtgg ccaccctggt gatgacgagg tccatgaacc ccgcttccca
      961 cgagtggcca gcggcgatgt cgacctgact cgtcgcatgc cctgattgcg tggcgagcca
     1021 ctcggcggtg accccggcct gcgcgatcgc ggtggtgttg aggtagacct catgacacga
     1081 ccggtgccac accaggatcg ggcgggtcgt gctcacctgg tcgaggaccg tgcggtcgag
     1141 cggaccatgc cacaacgggt ggtagcccca cgtgagcagc catccgtcgt cgcccgtgtc
     1201 agtgagggcc cgttcggcgg ccttcaagcg cgcgaggtac tcctcgggag tgtgggcggc
     1261 cggagaggtg tggccaggaa gcacccaatc ctcagtcgac gcgatctcca tcgacatggc
     1321 cgccgccccc gtcagggggt gcaggtgctg atccatgagg cctggcaaga cgacgtagtc
     1381 gctcagagtg tcatcgacct cgaagggcgc gtcgcccagt gccgcaacga ctgcgtcgaa
     1441 cgaacccacc gcgaggatgc ggtcccctcg gaccgcgacc gcttcggcag tggggaatcc
     1501 gggctccatc gtgtggaacc gccgagcacg cattactcgc acatcgctca tgtcaaccat
     1561 catccaccgg cagggaagac ccgtcctggc atctcggagg aactccgacc gatcgctgcg
     1621 tcgatggcgc ctcgcgggat ggcggcatac cgaccatcgg gtatgccgcc ctcccgcggg
     1681 tcagcctcgc cggatccgct cgcgaaccgc gcgcggggcg gaccgcaggg acgctacggc
     1741 ccggccccac cagcggcggg catcgccagg cagcaggagc gcgcgggcgc ggtggcgcca
     1801 ggagcgggtg cgcgccacgt cggagatgac gctgcgggtc aacggctcga ggtcgacggc
     1861 cagagaggtg ccgggccggg cgtaacgggc caactcgacg gtgtcgacga ctgtggcgag
     1921 ggactgccga gcgggctcgc cgaggtagcc ggcccgcgcc acgtgggcct ggctgtcgcg
     1981 cagcgttcca ccagcgggag tcgtgatccc gaggtcgtgc aactcgctgg tgaacagctt
     2041 ccagtgcgcc tcgatgacct cgttcggcgg gccgccgcgc cgggagcgga cccggcgcag
     2101 cagcgccgcc gtcaccggca gcacaagcgt tgcgacgacg accaggccga cgacgagcag
     2161 gaagaggtgg gggatctgcg cgacccacga gagcacgcgg tcgaggatcg ggaggtccaa
     2221 ctcggtccgc gcggtggtcg tgtcgtccgc gtcatccggc gcatcccggt cgggtcgatc
     2281 gagcgggtcc tcggtggatc gggccgtcga ggtcgcgagg ggggtgggcg tcaccggggg
     2341 aaccgtccac gacggcgccg tcccggtccg cacgctcggc gtcggctcga accgcaccca
     2401 gccggccccg gggaagtaca gctccggcca ggagtgggcg tcaccggccg tgaccgtcca
     2461 gaggccgccg tcctggctgc cggggaggaa gccgatggcc acgcgggccg ggattcccgc
     2521 ggcccgcgac atcatcacca tggccgaggt gaactgcacg caatagcccg tcttggtctg
     2581 caggaacgcg gagatcgggt cgcccacgac cccgctgacc agcggagcct ccgggagcgt
     2641 cagcgagtag gtgaaaccgc ccttggaccg cagccactcc tggatggcca cggcttcgtc
     2701 gtagggagtg gcggcccctg ccgtgacggt cgtcgtcagc gcgcggatgg cggcctccga
     2761 gcgcggatcg accgcgagcg cggcggacat cgtccggttg gtggggaggc ggtcaggccc
     2821 cggtctgccg tcgatcccct cgcgcaggag gtcggccgtg agcaccggct cgaggtaggt
     2881 ggtcgagtag gcgttcgggc gggtctgcac ggagacgtcg gaggtcgcga catcgacctg
     2941 ccacgagaca ccgctgaggt cggccgacag gatcggctgc ggggtggcca acgatggcgg
     3001 atccagggcg ttgctctcaa cgacgatcgt gcgttcggat cggaccaccg tgtcggcgag
     3061 gtccaaccgg gcactgcgac cgagggtggg cgccgggcgg ctccagttgg tgccgtcgta
     3121 tgccgtggcc gccagcaccc gcaggggcgc cgctgccgga gcattggtgc ggtaggtgag
     3181 gatcgtgccc gtgcccccgg aggtgaggct caacccgacg tcgagggtgg agttgaaccc
     3241 gaccttgccg acaccgcggc ctgcgccgtc ggcgctgcgg gccagcccgt ccaagaggta
     3301 ccgaggggga aggtgtggga gcaccgcggg aagggcgatc gccgccacca gcccgaccgc
     3361 gccgagccgc cgggcgatcg ttccgaagcg aagctctggc gcggagtcga gtgcagcgcc
     3421 agagatgggg gccatagggc gcgccgccgc cgaggcccac ccgcgcatcc ccacctgggc
     3481 ctgacgaccc aacatcaaca gccaggcgag cgcagcaatg atgaagtagg ccgggggcaa
     3541 cgccgagccg ctgttggcgg cggcggcgag gaacgcggtg agcaggggca ggccggccgc
     3601 cgccggcgcc gcccgggtga cggcgagggc atcgacgagg atggccaggc ccgctccaat
     3661 gagggccaag gtgacctcga cgccgacgtt ggccaggagg ggagccgagg actcggcgac
     3721 cgtggtgccg aacagcgtga acaactcggt gaaccgcccc acggtcgacc aggtgggcac
     3781 gccccaccac gtcgtcgaac cggcatactg ccaggtcgcc atgagcagca cgcccagcac
     3841 ctgcgcgggc acgatgagcc acccggaccg ggagacgaac cgtcggatca acgcgccgat
     3901 cccgaccgcc acgaagacga accagatggt ccgggcatac caggtctggg tcaccaccag
     3961 ggtggtcagc cccagcagcg ccgcgaggct ggccagggcc gcgagggccg ggttcgcgcc
     4021 acggcgggtc atcggctcgc ccccaaggcc acttgggcat cgcgtcggga gccgacctgg
     4081 ctccacgcgt gctccacggt gtcgtcgcgg gtcaccgtgg tggcccgcca accggccccg
     4141 cgcaacagat cgaccgcacg cgcggcctcc gaggcaaccg caccgcctcc cgcgttggcg
     4201 ccgcctcctg ccgggcgacc ggcgaacgat ccggtgtcga ggacgatgcc gagggcggtc
     4261 gctccgggat ggcgcagcgt ggccaccgac cgcaacgccg actcgtcgtg ggcggcgagg
     4321 acggcgacga gcaggccgcc cccagagagc agtccgagcg cagcccgggt gagtcgggcc
     4381 aacgccagcc cgctgtccgg ctcggcgacg gcgaggacgg ccaacgcctg cgccgggtcg
     4441 atcgggagcg catcgggccc gtccccgacg gtgtcggggg tgaggaggtg gacggcatac
     4501 ccgaggccgg acaggtgcac caccatcgag gcggcggcac tcaccgccca ctcgaaggaa
     4561 ctgccgacgc cgaccccggc gtgggcgtcg acccgcgggt cgagcaggac gatggcgcgg
     4621 cgcctggccg gtcgatcctc ctggcgcacc atgagttcgc ccgtgcgcgc agtggccggc
     4681 caatggatgc gccgcaggtc gtcgccgtcg cggtattcgc ggatcgactg gtcgtcctcg
     4741 ccgtgcaacg ccaccatgaa gggaatctca ccctcggcgc caacccccgt gcccggcggt
     4801 cgattgccgc cgagcgggta gacgcggggc aggacgagga cctcggtcgg ctcgccggcc
     4861 tcgacgaacc gctggctgag cccgaacggg tcgcgcagtt gcacggtgag cggcccgagc
     4921 aggtgacggc cgcgcagtgg tgggcggacg gcatacgtga gagcgcggcg ttcgcccgag
     4981 ccgagcgagc cgagcagcac ccgcggcc
//
LOCUS       DAOMZY010000268         4982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_15887_length_4982_cov_5.803126, whole genome shotgun sequence.
ACCESSION   DAOMZY010000268 DAOMZY010000000
VERSION     DAOMZY010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15887_length_4982_cov_5.803126"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2006
                     /locus_tag="PLK64_13315"
     CDS             <1..2006
                     /locus_tag="PLK64_13315"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HPV80769.1"
                     /translation="ASLKVRDRLANALGVDVASLPFVGELVQVRADQGQWRGAAERVL
                     RGFALSVLVPSELYAAASRWVDEHHLGARFVYYRVSDVVNRRPSVAVEDNTLAQVLEI
                     REGPHAAWLTAELRSRADHARVHHVDELREHRKAVTLLGQVKTDRRHEKDDRFALEDR
                     TRWVLGWSNADKVDALIERGATVQAQLTAARDALDAAKVDTDRHDAEAASVAQVLAVS
                     SWSLVDWRSAAQRIDELARERDVLQRGNAEVEALHARLAEVERARAAQGAVVTDLTEK
                     VGALKGEHRRNADQQRFAEADLDAVATDQLADLRTAYELLESRHDAPATLEACSLAER
                     DLQREFSASIQSHQEKQSAARERAAKRIAAFRQAWPSDTTELGADVLAAAEYRALRDR
                     ITGDDLPRFEAEFKRQLNTNTINDIAGFQAWLDQSAQTIRSRIDTINESLSAIDYSPG
                     TRIRLLTEPSTNQEVRAFRDDLRACTDDALSTDDQYSEERFRRVKAIIERFRGREGLT
                     EVDKRWTELVTDVRSWFVFAASEREADSGVEREHYTDSDGKSGGQKEKLAYTILAASL
                     AYQFGLEWGVPKSRDFRFAVIDEAFGRGSDASTRYALDLFAKLGLQLLVVTPLQKVHV
                     IEPYVSAVGFVENRTGQRSRLQTLTIEEYHAERAEHGRAGRPS"
     gene            2003..3169
                     /locus_tag="PLK64_13320"
     CDS             2003..3169
                     /locus_tag="PLK64_13320"
                     /inference="COORDINATES: protein motif:HMM:NF023223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2220 family protein"
                     /protein_id="HPV80770.1"
                     /translation="MSAWTTSADVRARAQKRWDAGELLSAHATGAPAPVLDLPVRGPT
                     PREVSAQLSQVRSWRDALVAGSRGGTCYDLTERSIGGRVIGSASLPDRVKVTAYDQWW
                     RLLGVTEQVRAFDALLARTREAHPALVDWVARSPLRALAVSTDWEHLLAAHAWLVESA
                     GQGRHLREVTAPGVDTKFIEGHTRVLGEWLDATVAFDPRHSAARRFARRYGFAEAPQL
                     LRLRVDAGLGVLPPGVSEVGLRVAEAGALAVAPSQVLIVENLTTYLAVPVPEGGVVVW
                     GQGFDAGRLGRMPWLGVALRVRYWGDLDTHGFAILDLVRSQVPQVTSVLMDLDTLLHH
                     RSRWVPEPKQTRADLVHLTGGERALYEDLVEDVHGPSVRLEQERIDWAWVLERL"
     gene            3507..4430
                     /gene="mmuM"
                     /locus_tag="PLK64_13325"
     CDS             3507..4430
                     /gene="mmuM"
                     /locus_tag="PLK64_13325"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047150 - betaine-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HPV80771.1"
                     /translation="MNLTEPVILDGGLSNALEARGVDLSSDLWTARLLLDDPDQIAAV
                     HRDYYLAGADVVTTASYQASVPTLMAAGLTEREAERALTSSVTLATQVRDEIAAETTR
                     VRPPLVAASVGPYGAALADGSEYRGRYGVSAATLRDFHAPRLELLAAAAPDLLAVETI
                     PDADEAEVLVPLLDDLGLPTWFSYSVAGTATRAGQPLSEAYAVLADSTSIIAAGINCS
                     APNDVMDAVRLATQATARPSVAYPNLGETWDSATHTWSGDAAFDTDLAADWVAAGARL
                     VGGCCRVGTDQITRLAKRLVPASAPGSTPLQ"
     gene            4486..>4982
                     /locus_tag="PLK64_13330"
     CDS             4486..>4982
                     /locus_tag="PLK64_13330"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPV80772.1"
                     /translation="MSFLVAYSDTPAGRDALALGVRAAHAADTTLDIVVVRHAENATP
                     PQDGFTRYLEATIDTWLAKAGDKARATLGDDHVRVHQRLAESTAQGLFDAASDLGSEV
                     IFVGGAKDGLRGRLALSSLASVLVHASPVAVALAPRKSRKLHLAGISRISVGIEPGHL
                     GPVEV"
BASE COUNT          759 a         1805 c         1672 g          746 t
ORIGIN      
        1 ccgccagcct gaaggtgcgg gatcgcctcg cgaatgcgct cggtgttgat gtcgccagct
       61 tgccgttcgt gggcgaactc gttcaggtcc gagctgatca ggggcagtgg cgtggggcag
      121 ccgagcgcgt ccttcgaggg ttcgccctgt cggttctcgt ccccagtgag ctgtatgccg
      181 ctgcgtcccg atgggttgac gagcatcacc tcggtgccag gttcgtctac taccgcgtga
      241 gcgacgtcgt gaaccggcga ccgtccgtgg ccgtggagga caacaccttg gcgcaggtgc
      301 ttgagatccg ggagggaccg cacgccgcct ggctcaccgc tgaactgcga tcgcgggccg
      361 accacgcacg ggtgcaccac gtcgacgagt tgcgtgagca ccgcaaggcg gtgaccctcc
      421 tcgggcaggt aaagacggac cgccgtcacg agaaggacga ccggttcgcg ctcgaggacc
      481 gcacgcggtg ggtcctcggc tggtccaacg ccgacaaggt cgacgccctc atcgaacgcg
      541 gtgccaccgt gcaggcccag ttgaccgccg cccgtgacgc cttggacgcg gccaaggtcg
      601 acaccgatcg acatgatgcc gaggccgcca gcgtcgcaca ggtcctggcc gtatcaagct
      661 ggtcgctcgt cgactggcgc tccgccgccc agcgcatcga cgagctcgcg cgcgagcgcg
      721 acgtgctcca gcgcggcaac gccgaggtcg aggcgctgca cgctcggctc gccgaggtcg
      781 agcgggcgcg cgccgctcag ggcgccgtgg tcaccgacct caccgagaag gttggcgccc
      841 tcaagggtga gcaccgcagg aacgccgatc agcaacggtt cgccgaggca gacctcgatg
      901 cagtggcgac cgaccagctc gccgacctgc gaacggcata cgaactgctt gagagtcgac
      961 atgacgctcc ggcgaccctg gaggcctgct cactggccga acgcgatctc caacgtgagt
     1021 tctctgcgtc catccagtcc caccaggaga agcagtcagc ggcgcgtgag cgggccgcca
     1081 aacgcattgc cgcgttccgt caggcctggc ccagcgacac caccgaactc ggcgccgacg
     1141 tgctcgcggc cgccgagtac cgcgcgctgc gcgaccggat caccggtgat gacctgcccc
     1201 gcttcgaggc cgagttcaag cggcagctca acacgaacac gatcaacgac atcgccgggt
     1261 tccaggcctg gctcgaccag tcggcccaga cgatccgctc gcgcatcgac accatcaacg
     1321 agtcactgtc cgcgatcgac tacagccccg gcacacgcat ccggctgctt accgaaccga
     1381 gcaccaacca ggaggttcgg gcgttccgcg acgacctgcg ggcctgcacc gacgacgcgc
     1441 tgagcaccga cgaccagtac tccgaggaac gcttccgccg cgtcaaggcc atcatcgagc
     1501 gcttccgcgg gcgcgagggt ttgaccgagg tcgacaagcg ctggaccgag cttgtcaccg
     1561 acgtccgcag ctggttcgtc ttcgcggcat ccgaacgcga ggccgacagc ggcgttgagc
     1621 gtgagcacta caccgactcc gacggcaagt ccggtgggca gaaggagaag ctcgcctaca
     1681 cgatcctcgc ggcgtcgtta gcctaccagt tcgggctgga gtggggtgtg cccaagtccc
     1741 gggacttccg gttcgccgtc atcgacgagg ccttcggccg tggctcggac gcctccaccc
     1801 gctatgccct ggacctgttc gccaagctgg gcctgcaact gctcgtggtg acgcctctgc
     1861 agaaggtcca tgtcatcgag ccctacgtca gcgccgtcgg gttcgtcgag aaccgcacgg
     1921 ggcagcggtc ccgcctccag acgctcacca tcgaggagta ccacgccgag cgcgccgagc
     1981 acgggcgtgc gggtcgcccc tcgtgagcgc gtggacgacg tcggcggatg ttcgcgctcg
     2041 tgcccaaaag cgctgggatg ccggtgagtt gctcagtgca cacgcgaccg gggcaccggc
     2101 tcccgtgctt gacctaccgg tgcgcgggcc gaccccgcga gaggtgtcgg cgcagctgtc
     2161 gcaggtgcgg tcgtggcgcg acgcgctcgt ggccggcagt cgcggcggca cttgctacga
     2221 cctgaccgaa cggtccatcg ggggtcgggt catcgggtcc gcaagcctgc ccgaccgggt
     2281 gaaggtcacg gcatacgacc agtggtggcg gctgctcggc gtgacggagc aggtgcgcgc
     2341 gttcgacgca ctgctggcgc gcacccggga agcccacccc gcgctggtcg actgggtggc
     2401 ccggtctccc ttgcgggcgt tggcagtgtc gaccgactgg gaacacctgc tcgcggcgca
     2461 tgcctggctc gtcgagtctg cagggcaggg tcgccacctg cgtgaggtga ccgcacccgg
     2521 cgtggacacg aagttcatcg aggggcacac gcgcgtgctg ggcgagtggc tggacgcgac
     2581 ggtggcgttt gacccacgcc attcggcggc gcggcgtttc gcccggaggt acggcttcgc
     2641 cgaagctcct cagttgctgc ggctacgagt tgatgccggt ctgggcgtgc tgcctcccgg
     2701 agttagtgag gtgggactcc gggtggcgga ggcaggagcg cttgccgtgg cgccctctca
     2761 ggtgctcatc gtcgagaacc tcacgaccta cctcgcggtg cccgttcctg agggaggtgt
     2821 ggtcgtctgg gggcagggct ttgatgccgg ccgcctcggt cggatgccgt ggctcggcgt
     2881 tgcgctccgc gtgcgctact ggggtgacct ggacacccac ggtttcgcga tcctcgacct
     2941 cgtgcgctcg caggtgccgc aggtgacgtc ggtgttgatg gacctcgaca ccctgctgca
     3001 ccaccgctca cgctgggtgc cggagcccaa gcagacccgc gccgacctgg tgcacctcac
     3061 tgggggtgag cgggcgttgt acgaagacct cgtcgaggat gtccacggcc cctccgtgcg
     3121 tctcgagcag gagcggatcg actgggcctg ggtgctcgag cggctgtgac cgaaacctcc
     3181 catcacccct tcaacccgtt cctggccagg ggatcccttc caagcaaagc gcggcgacag
     3241 cggtctgggg ggcaacggca tccgaactca gcgagcgcgg cggtgtctct gtggaggccg
     3301 acggtctcgc gagcgtggtc gatgtgcggt cgtcgtgcat ccacgcaatc ctttggcgca
     3361 tgccctcagc atcaagccga ccatcgacga ggacgcccgc atcgcgtacg agcggttcct
     3421 ccagggtgcg ccgctgccgg gtcgtgagca cggctgaacc gctccgacaa gagcggccgc
     3481 aggagcccgg tgggaggatc ggcgccatga acctcactga gccggtgatc cttgacggtg
     3541 gcctgtccaa cgccctcgag gcccgaggcg tcgacctgtc ctccgacctc tggacggctc
     3601 gtctgctgct cgatgaccca gaccagatcg ccgccgtcca ccgcgactac tacctcgccg
     3661 gtgccgacgt cgtcacgacg gcgagctacc aggccagcgt cccgaccctg atggcggccg
     3721 ggctcaccga gcgcgaggcc gagcgggccc tcacctcgag cgtcaccctg gccacgcagg
     3781 tgcgcgacga gatcgccgcc gaaaccaccc gtgtcaggcc gcccctcgtc gccgcctccg
     3841 tcggcccgta cggcgccgcg ctcgccgacg gctccgagta ccgcggccgc tacggcgtca
     3901 gcgcggccac cctgcgtgac ttccacgcgc cacgactcga actcctcgcc gcagcagccc
     3961 cggacctcct cgccgtcgag accatccctg acgccgacga ggcggaggtg ctcgtccccc
     4021 tcctcgacga cctcggcctg ccgacctggt tctcctactc cgtcgccggc accgccaccc
     4081 gcgcgggcca gccgctcagc gaggcctacg cggtcctggc cgacagcacg agcatcatcg
     4141 ccgccggcat caactgctcc gcaccgaacg acgtgatgga tgccgttcgc ctcgcgaccc
     4201 aggccaccgc gcgcccgtcc gtggcctacc cgaacctcgg tgagacctgg gacagtgcga
     4261 cccacacctg gagcggggac gccgcgttcg acaccgacct cgccgccgac tgggtcgccg
     4321 ccggcgcccg cctcgtcggt gggtgctgcc gcgtcggaac cgaccagatc acccggctgg
     4381 cgaagcgtct cgttccagcc agcgccccag gctcaacgcc actacagtga cgcccagtct
     4441 gtttgcgggc acaccgcgga ccaccggggc aaaggggctc tcagcatgtc gtttctcgtc
     4501 gcgtactccg acacccccgc ggggcgagac gccctggcgc taggggtccg agcagcccac
     4561 gccgccgaca ccaccttgga catcgtcgtg gtgcgccacg ccgagaacgc cacgcccccg
     4621 caggacgggt tcacccgcta cctcgaggcc accatcgaca cctggttggc caaggccggc
     4681 gacaaggccc gcgccaccct cggcgacgac cacgtgaggg tgcaccagcg cctcgccgag
     4741 agcaccgcgc agggactgtt cgacgcggca tccgatctcg ggtccgaggt catcttcgtc
     4801 gggggcgcca aggacggctt gcgcggccgg ctcgccctgt cgtcgctggc cagcgtcctc
     4861 gtgcacgcca gcccggtcgc cgtggcgctt gccccgcgca agagccgcaa gctgcacctc
     4921 gccggcatct cccgcatctc ggtgggcatc gaacccggcc acctcggccc cgtcgaggtg
     4981 ca
//
LOCUS       DAOMZY010000269         4979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_15902_length_4979_cov_4.769293, whole genome shotgun sequence.
ACCESSION   DAOMZY010000269 DAOMZY010000000
VERSION     DAOMZY010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15902_length_4979_cov_4.769293"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PLK64_13335"
     CDS             <1..724
                     /locus_tag="PLK64_13335"
                     /inference="COORDINATES: protein motif:HMM:NF012948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HPV80773.1"
                     /translation="PETVDTPIPSTVYTSVTNSSALGDDPTVLTLQTPVASLVLLKSI
                     TNVVDTTGNGLIGVGDTVTYTFKVTNTGNVTLAPVTITDEKLGLTNWSCLATLAPGQT
                     ANCTSTANYIIQTVDVQNQGIVNTATATGTPPTRPAGAPVIPPATDVSDTGTYPTTPG
                     HVSTVTNPATNETTIPTSITTTVTNSPTDPTSDPTVLSLAPYTLPRTGGVGVNLYTSG
                     GALIVGGGLVWLLLGLRRRHAA"
     gene            complement(840..3236)
                     /locus_tag="PLK64_13340"
     CDS             complement(840..3236)
                     /locus_tag="PLK64_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPV80774.1"
                     /translation="MAQHQGTHPADTHPADTHDLIRVHGARENNLKNVDVELPKRRLT
                     VFTGVSGSGKSSLVFNTIAAESQRMINETYSAFVQGFMPNLARPDVDLLDGLTTAIIV
                     DQQRLGADPRSTVGTATDALALLRILFSRLGQPHIGSAQAFSFNVASISGAGAVTIQR
                     GGQAVKERREFSITGGMCPRCEGRGAVTDFDLTALYDDSKSLREGALSIPGYSMDGWY
                     GRIFAGSGFFDMDKPIRDFTKRELADLLHKEPTKIKVEGINLTYEGIIPKIQKSFLAK
                     DVEAMQPHIRAFVERAVTFQTCPECAGTRLNEGARSSKIAGINIADACRMQISDLADW
                     VATLDEPSVAPLLATLRHTLDSFVEIGLGYLSLERPAGTLSGGEAQRTKMIRHLGSAL
                     TDVTYVFDEPTAGLHPHDIQRMNELLIRLRDKGNTVLVVEHKPEVIEIADHIVDLGPG
                     AGTAGGEVVFEGSVAALRASDTVTGRHLDDRSRLKDSVRMPSGSLPVRGAAANNLREV
                     DVDIPLGVLVVLTGVAGSGKSSLVDGSVSGLDGVVSIDQAPIRGSRRSNPATYTGMLE
                     PIRKAFAKANGVKPALFSSNSEGACPACNGAGVIYTDLAMMAGVATTCEECEGRRFQA
                     AVLDYRLGGLNIAEVLDLPVAQAAEFFGGSSGSGQAAIPAAAAILGRLADVGLGYVRL
                     GQPLTTLSGGERQRLKLASHMGEKGQIYVLDEPTSGLHLADVQQLLGLLDRLVDSGKS
                     VVVVEHHQAVMAHADWIIDLGPGAGHDGGRIVFEGTPADLVAAKSTLTGTHLAAYIKA
                     "
     assembly_gap    3248..3347
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3566..>4979)
                     /locus_tag="PLK64_13345"
     CDS             complement(3566..>4979)
                     /locus_tag="PLK64_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80775.1"
                     /translation="REHHLGAVLCSDGGVGMVTPGQGLPTADDARKACETPVVPGGGG
                     GGDKGNGGTLPDLLGTSSGMPSASSISCSAQGGANPYAQEAAKEPRVTDGDIVAGLIG
                     SWLTGVGVMYGTAKGAGVAPTAAGGGLALGAGSVGIIAWGKAAANALGELMEGRYRDA
                     AGELAGDAAHFAQAAEANAQAAEQAAAASGSPEAQTAAKEARAAAEAAAKAAEAAKGA
                     AKQAAEAKTRAEVAAAYEAAKKADTEAKKKAEEAAKAREKAQSAGQTGTTNTGGTGGS
                     GTTTTPSGQSAESTCEKVRRQVWECEQGGWKSFSCQELARMLQGCRVDLTVALVDGDS
                     SLCGLPTVSTEDLVRARDEACGLLVGHPMPGVDPCTTTVEGSPEIRGSCDPTIAYGGC
                     PDEPDVVVMDDDTICFPAPPSAPPMPCVDPTPGGGPIVGETKTWSPSPSGFLQDSFGG
                     VFVVSRGGIIVIGQGYPPHP"
BASE COUNT          733 a         1733 c         1625 g          788 t
ORIGIN      
        1 ccccgagacg gtcgacactc cgatcccgtc gacggtctac acctccgtca ccaattcctc
       61 ggcgctgggc gatgacccaa cggtcctcac cctccagacg ccggtggcga gcctcgtcct
      121 gctcaagtcg atcaccaatg tggtcgacac gacgggcaac ggcctgatcg gcgtaggcga
      181 cacggtgacc tacaccttca aggtgacgaa cacgggcaac gtcaccctcg cgcccgtgac
      241 gatcaccgac gagaagttgg ggctgaccaa ctggtcctgt ctggcgacgc tggcgccggg
      301 ccagacggcg aactgcacat ccacggcgaa ctacatcatc cagaccgtgg atgtgcagaa
      361 ccaaggcatc gtcaacacgg cgaccgcaac cggcaccccg ccaaccaggc ccgcgggcgc
      421 gccggtcatc cccccggcca cggacgtgtc tgacaccggg acctacccga cgacgccggg
      481 gcacgtaagc acggtgacca acccggccac caacgagacc acgattccga ccagcatcac
      541 gaccacggtg accaacagcc cgaccgaccc gacgagcgac ccgaccgtcc tctcgctggc
      601 gccctacacg ttgccgcgca ccggtggcgt cggggtgaac ctctacacct cgggaggcgc
      661 gttgatcgtc gggggcggac tggtctggtt gctcctaggc ctgcgccgcc gccacgccgc
      721 ctgactgaca acgcgctcag acgccggcgc gcggcatacc agggagagcg acaaggtatg
      781 ccgcgcgtcg gtccgtttct gcgggcactt cgcccgctct gccgcgcgcg ggctcgcagt
      841 taggccttga tgtatgccgc gaggtgcgtt cccgtgaggg tggacttcgc ggcgaccagg
      901 tcggcaggcg tgccctcgaa gacgatccgg cccccgtcat gtcccgcgcc gggcccgagg
      961 tcgatgatcc agtcggcgtg cgccatgacc gcctggtggt gctcgacgac aacgactgac
     1021 ttgccggagt cgacgagccg gtcgagcaga ccgagcaact gctggacatc ggccaggtgc
     1081 agaccagatg tcggctcgtc gagcacgtaa atctggccct tctcgcccat gtggctggcg
     1141 agcttgaggc gctgacgctc gccgcccgag agcgtggtga ggggctggcc gagccggaca
     1201 tagcccaacc cgacgtcggc caaccggccg aggatcgccg cggcggccgg gatcgccgcc
     1261 tgcccggagc cggagctgcc tccgaagaac tcggccgcct gggcaaccgg cagatcgaga
     1321 acctcggcga tgttgagccc accgagccgg tagtccagca ccgccgcctg gaaccgccgc
     1381 ccctcgcact cctcacaggt cgtcgccacc cctgccatca tcgccaggtc ggtgtagatg
     1441 accccggcgc cgttgcaggc cgggcaggcg ccctccgagt tcgagctgaa cagagccggc
     1501 ttcacgccgt tcgccttggc gaaagccttg cggatcggct cgagcatccc cgtataggtc
     1561 gccgggttgc tgcgccgaga cccacggatg ggtgcctggt cgatcgatac caccccgtcg
     1621 agcccggaca ccgagccgtc gacgagcgag ctcttgcccg acccggccac accggtcagg
     1681 acgacgagca cccccaacgg gatgtcgaca tcgacctcgc gcaggttgtt ggccgcggcg
     1741 ccgcgcacgg gaagcgagcc ggacggcata cgcaccgagt ctttgagccg cgagcggtcg
     1801 tccaggtgcc tcccggtgac cgtgtcactc gcccgcagcg ccgcgaccga cccctcgaag
     1861 accacctcgc cgcccgcggt ccccgcgccc ggaccgaggt cgacgatgtg gtcggcgatc
     1921 tcgatgactt cgggcttgtg ctcgacgacg aggacggtgt ttcccttgtc ccgcaaccgg
     1981 atcagcagtt cgttcatccg ctggatgtcg tgcgggtgca ggcccgcggt cggctcgtcg
     2041 aagacgtagg tgacgtcggt caacgccgac cccaggtgcc ggatcatctt ggtccgctgt
     2101 gcctcgccgc cggagagggt gcccgccggc cgctcaaggc tgaggtagcc gagcccgatc
     2161 tcgacaaacg agtcgagggt atgccgcagg gtcgccagca gcggcgcgac cgacggctcg
     2221 tcgagggtcg ccacccagtc ggcgaggtcg ctgatctgca tccggcaggc gtcggcgatg
     2281 ttgatgccgg cgatcttcga cgagcgcgcg ccctcattga ggcgggtccc cgcacactcg
     2341 gggcaggtct ggaaggtgac cgcccgctcg acgaaggccc ggatgtgcgg ctgcatcgcc
     2401 tcaacgtctt tggcgaggaa agacttctgg atcttgggga tgatgccctc gtaggtgagg
     2461 ttgatgccct cgaccttgat cttggtgggc tccttgtgca gcaggtcggc caactcgcgc
     2521 ttggtgaaat cgcggatcgg cttgtccatg tcaaagaacc cgctgccggc gaagatccgg
     2581 ccgtaccacc cgtccatgct gtagcccggg atcgacagcg cgccctcacg cagcgacttg
     2641 ctgtcgtcat agagggccgt gagatcgaag tcggtcaccg ccccgcggcc ctcgcagcgc
     2701 gggcacatcc caccagtgat cgagaactcg cgccgttcct tgacggcctg gccaccgcgt
     2761 tggatcgtca ctgcgcctgc gccgctgatc gatgccacat tgaacgaaaa cgcttgtgct
     2821 gagccgatat gcggctgccc gagccggctg aagaggatcc gcagcagcgc gagggcgtcg
     2881 gtggcggtgc ccaccgtcga gcgcgggtcg gcgccgagcc gctgttggtc gacgatgatc
     2941 gccgtcgtca gaccgtcgag caggtcgacg tcggggcgcg ccaggttggg catgaagccc
     3001 tggacaaacg cgctgtaagt ctcgttgatc atccgctgcg actcggccgc gatcgtgttg
     3061 aagaccagcg agctcttgcc cgaccccgac acccccgtga acacggtgag ccgacgcttg
     3121 ggcagctcga cgtcgacgtt cttgaggttg ttctcccgcg caccatggac cctgatgagg
     3181 tcgtgggtgt cggcagggtg ggtgtcagca gggtgggtcc cttggtgctg cgccatcaac
     3241 ggcccttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngca gggtgggtgt
     3361 cagcagggtg ggtcccttgg tgctgcgcca tcaacggccc ttcgctcggt gctcccgtca
     3421 ggcgctcaga ctaaccgcgg tgagcacacg gcataccgtt gcttggtgcc ggcggggaac
     3481 ctcacgctca cacgcgctcc cgtcggcgac cattggtatg ccgtgtgccc agcgcgcgac
     3541 tcggcgggca ccggccttgg tcacctcacg ggtggggtgg gtacccctga ccgatgacga
     3601 tgatcccccc tcggctcacg acgaacaccc cgccgaagct gtcctggagg aagccgctcg
     3661 ggcttgggct ccacgtcttg gtctcgccga cgatcggtcc gccgccgggc gttgggtcga
     3721 cgcacggcat cggcggtgcg ctcggaggcg cagggaagca aatggtgtcg tcgtccatga
     3781 ccacgacgtc cggctcgtcg gggcaaccgc cgtaggcgat cgtgggatcg caactgcccc
     3841 ggatctccgg cgacccttcg acggtcgtcg tacaggggtc cacgcctggc atcggatgcc
     3901 cgacgagcag cccgcacgcc tcgtcgcggg cccgcaccag gtcctcggtt gacacggtgg
     3961 gtagcccaca cagggacgag tcgccatcga cgagggccac ggtcaggtcg acccggcagc
     4021 cctgcaacat ccgcgccaac tcctggcacg agaagctctt ccacccgcct tgctcgcact
     4081 cccagacctg gcggcggacc ttctcacagg tgctctccgc ggactggccg ctcggggttg
     4141 tcgtcgtgcc ggaccccccg gtgccgccgg tgttcgtcgt gccggtctgg ccggcacttt
     4201 gggccttctc gcgggccttg gccgcttcct cggccttctt cttggcctcg gtgtcggcct
     4261 tcttggccgc ttcgtaggca gcggcaacct cggcgcgagt cttggcctca gcggcttgct
     4321 tggcggcacc cttggcggct tcggcggcct tggccgcggc ttcggcggca gcccgggcct
     4381 ccttggctgc ggtctgggcc tcggggctgc ccgacgcggc agcggcttgt tcagcggcct
     4441 gcgcgttggc ctcggcggcc tgcgcgaagt gggcggcatc cccggccaat tcccctgcag
     4501 cgtctcggta tcgaccttcc attagctcac ccagagcatt cgctgccgcc ttcccccagg
     4561 cgatgatgcc gaccgagccg gcgccgaggg ccagtccgcc acccgccgcg gtgggggcca
     4621 caccagcgcc ctttgcggtg ccatacatca ccccaacacc tgtcagccat gagccgatca
     4681 gcccggccac gatgtcaccg tcggtcactc gcggctcttt cgcggcctct tgtgcgtagg
     4741 ggttcgcgcc gccctgggca ctacacgaga tcgagctcgc ggacggcata ccggaggagg
     4801 ttcccaacag gtcggggagg gtgccgccgt tccccttgtc cccgccgcca ccgccgccgg
     4861 gcacgacggg cgtctcgcag gccttgcggg cgtcgtcggc cgtggggagg ccttggcccg
     4921 gggtgaccat gcccaccccg ccgtcggagc agaggaccgc tccgaggtgg tgttcacgg
//
LOCUS       DAOMZY010000270         4953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_16007_length_4953_cov_5.631278, whole genome shotgun sequence.
ACCESSION   DAOMZY010000270 DAOMZY010000000
VERSION     DAOMZY010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16007_length_4953_cov_5.631278"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PLK64_13350"
     CDS             complement(<1..403)
                     /locus_tag="PLK64_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HPV80776.1"
                     /translation="MRVAIVGAGSVGRSIARELLHNGHQVLLIDRDADDVVASRVPDA
                     TWLLADACEISALEEAHLEACDVVVAATGDDKANLVVSLLAKTEFGVPRTVARVNNPR
                     NEWMFDEAWGVDVAVSTPRLMTALVEEAVSIG"
     gene            complement(440..1102)
                     /locus_tag="PLK64_13355"
     CDS             complement(440..1102)
                     /locus_tag="PLK64_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HPV80777.1"
                     /translation="MHFVIMGCGRVGATLARSLSDLGHDVAVIDMDPTSFRRLGSSFE
                     GRTITGMGFDRGVLVAAGIREAYAFAAVSSGDNSNILAARVARETFGVEHVAARIYDP
                     RRAAVYQRLGIPTVATVRWTADQIIHRLLPTGSVPAFTDPSGQVVLAEVPVAAAWVGR
                     RVTTIERLASTRVAFLTRLGEGMVPDADTVLQDGDLMHVCAPATELDRLERVFAARPP
                     HE"
     gene            1239..3203
                     /locus_tag="PLK64_13360"
     CDS             1239..3203
                     /locus_tag="PLK64_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HPV80778.1"
                     /translation="MRSDRLGETLLPKKLALPIFASDALSSVAYAPDEILIMLGLAGG
                     AFAAMHSWQITLGVIFVMVIVVASYRQNVHAYPSGGGDYEVATENLGPKAGLTVASAL
                     LVDYVLTVAVSISSGVQNARAALPFISGHEVVIAVGLIVLLMAMNLRGVRESGKAFAI
                     PVYAFMFGIIGMGVWGFIKGLTGTLGQAPSAGLELVAEPGHDAVGLAAMFLLLRAFSS
                     GCAALTGVEAISNGVPAFKKPKSANAAATLLLMGVISVTMLASIIALSRMTGVQMADS
                     SAVGEVLLRDGVPVGDTYVQDTVIGQLAKTVFSDFPIGLYFVTAVTGIILVLAANTAF
                     NGFPVLGSILAKDGFLPRQLQSRGDRLAFSNGIAILAAAAIFLVIAYNADVTQLIQLY
                     IVGVFVSFSMSQTGMIRHWNRLLKEETDPKARSKMFRARIINAVGAAMSITVLLVVLV
                     TKFQAGAKYAILAMAVLYVMMLAINRHYRHVSQELALRDDLREAILPSRVHALVLVSK
                     LHKPAMRAIAYARATRPSVLEALTVEVDPEETAALGREWIARDVPVTLKIVASPYREI
                     TRPIIDYVKSLRTDNPRDVVIVYVPEYVVGHWWEQLLHNQSALRLRVRLHFIPGVMVA
                     SVPWQLRSSEGQSDDWDDRSVPGRAGNIAP"
     gene            3200..4483
                     /locus_tag="PLK64_13365"
     CDS             3200..4483
                     /locus_tag="PLK64_13365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HPV80779.1"
                     /translation="MSPVSRSSGRQRRYAGPRKPAGPRGESLVGEVFEVEVGPVAHGG
                     FCVARHDGRVVFVRHTLPGERVRAKVTEGGSEDRFLRADAVEVLSPSADRVDPVCRHA
                     GPGGCGGCDWQHVDLAAQRRLKGAVVAEQLWRLAGIDRSVEVLPVPGDVDGFGWRTRV
                     EFAVDRAGRAGLRPTRSHAVLPIMQCPIADPRVLASGVLADDWSGAKAVDVVAPSGGG
                     AVVVVPIPAAAGSVPEIRERVESASWTGEFDVSARGFWQVHPGAAATFVDRVLAELNP
                     RPGERVLDLYAGVGLFAVPLADAVGPGGAVLAVEGDVLACELAVRNAGNRTWIEVVPD
                     RVEEVLASTSPDAWSSADLVVLDPPRTGAGRAVIEAVARLQPRAVAYVACDPAALARD
                     LGYAREAGYVLGGLTAYDAFPMTHHVECIAVLKPT"
     gene            complement(4530..4928)
                     /locus_tag="PLK64_13370"
     CDS             complement(4530..4928)
                     /locus_tag="PLK64_13370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HPV80780.1"
                     /translation="MDPNVFISATITPNGALGLIIRLIDEGTLVPVVTQHLVDEVVYV
                     LARPKLSRYVKPGAGAAFEDLIRRLAEWHADIANPPSVTRDPKDDYLVALALGTAADA
                     LTSGDDDLHAAKNLGIEVLTPRELLTLFGL"
BASE COUNT          720 a         1641 c         1737 g          855 t
ORIGIN      
        1 caccgatgct gacggcctcc tcgacgagcg cggtcatcag acgaggggtc gacacggcga
       61 cgtccacgcc ccaggcctca tcgaacatcc actcgttccg gggattgttg acgcgggcga
      121 cggtccgggg gacgccgaac tccgtcttgg ccagcagcga gacgacgagg ttggccttgt
      181 cgtcaccggt tgcggcgacg acgacgtcac aggcctccag gtgggcttcc tcgagggcag
      241 agatctcaca ggcgtcggcg agcagccagg tggcgtcggg gacgcgggag gccacgacat
      301 cgtcggcgtc acggtcgatg agcaacacct gatggccgtt gtgcaggagc tcgcgagcga
      361 tggatcgccc gacgctgccg gctccgacga tggcgacgcg catgggatgt ccttggctgg
      421 gctggagatg gcggggtggt tattcgtgcg gcgggcgcgc ggcgaagacg cgctccaacc
      481 ggtcgagctc ggtcgcgggc gcgcagacgt gcatgagatc gccgtcctgc aagaccgtgt
      541 cggcgtccgg caccatgcct tcgccgagcc gggtgaggaa ggcgacccgc gtcgaggcca
      601 gccgttcgat cgtcgtgacg cgacgaccga cccaggccgc ggccacgggg acctcggcga
      661 ggaccacctg gccgctgggg tcggtgaacg caggcaccga gccggtcggc agcaggcggt
      721 ggatgatctg atcggccgtc cagcgcaccg tcgcgacggt cgggatgccg aggcgctgat
      781 agaccgcggc gcggcgcggg tcatagatgc gcgcggcgac gtgctcgacc ccgaaggtct
      841 cgcgggcgac ccgggcggcc aggatgttgg agttgtcacc gctggagacc gccgcgaagg
      901 catatgcctc tcgaatcccg gccgccacga gaactccgcg gtcgaagccc atgccggtga
      961 tggtccgccc ctcgaacgaa ctgcccagcc gccggaacga ggtggggtcc atgtcgatga
     1021 cggcgacgtc gtgtccgagg tcggacagac tccgcgcgag ggtcgcacct acgcgcccgc
     1081 agcccatgat gacgaagtgc acgctccgac gctacaccgg cggtaacgcg catctcacac
     1141 cggcgacgac ggtgcgcagg tatgccgcgc aacggcatac catgcgattt gtgcctgctc
     1201 tcggccaagt agccaagcgg atcctcgtcg gtcgtgccat gcgcagcgat cggctcgggg
     1261 agaccctcct gcccaagaag ctcgccctgc cgatcttcgc cagtgacgcc ctgtcgtcgg
     1321 tagcctatgc ccccgacgag atcctcatca tgttggggtt ggctggcggt gccttcgccg
     1381 ccatgcactc gtggcagatc acgctcggtg tcatcttcgt catggtcatc gtcgtggcgt
     1441 cctaccgcca gaacgtccat gcctacccgt ccggtggcgg cgactacgag gtggcgacgg
     1501 agaacctcgg ccccaaggcc gggctgaccg tcgcctcggc cctgctggtc gactacgtcc
     1561 tcacggtcgc ggtgtcgatc tcgtccggcg tccaaaacgc gagggcggcc cttcccttta
     1621 tctccgggca tgaggtcgtc atcgcagtgg ggctcatcgt gctcctcatg gcgatgaact
     1681 tgcgcggagt tcgcgaatcg gggaaggcct tcgcgattcc tgtctacgcc ttcatgtttg
     1741 gcatcatcgg catgggtgtg tgggggttca tcaaggggtt gaccggcacc ttgggccagg
     1801 ccccgagcgc cggcctcgaa ctcgtcgccg agccagggca cgacgcggtg gggctggccg
     1861 ccatgttcct gctgctgcgg gccttctcct ctgggtgcgc cgcgctcacc ggggtggagg
     1921 cgatctccaa cggggtccct gccttcaaga agcccaagag cgccaatgcg gcggcgaccc
     1981 tgctgctcat gggtgtcatc tcggtcacca tgcttgcgtc gatcatcgcg ctgagccgga
     2041 tgaccggggt gcagatggcc gacagcagtg cggtgggtga ggtcctgctg cgcgacggtg
     2101 tgccggtcgg tgatacctac gtccaggaca ccgtcatcgg ccagttggcc aagacggtct
     2161 tctccgactt cccgatcggg ctctacttcg tcacggccgt caccgggatc atcctcgtcc
     2221 tcgcggctaa cacggcgttc aacggcttcc cggtgctggg ctcgatcctc gcgaaggacg
     2281 gcttcctgcc gcggcagttg cagtcgcgcg gtgaccggct ggccttctcc aacggcattg
     2341 cgatcctcgc cgcggccgca atcttcctcg tcatcgccta caacgccgac gtcacccagc
     2401 tcatccagct ctacatcgtc ggcgtcttcg tgtcgttctc aatgagccag acgggcatga
     2461 tccggcactg gaaccggctg ctcaaggagg agaccgaccc caaggcccgg tcgaagatgt
     2521 tccgggcccg gatcatcaac gcggtcggcg ccgccatgtc gatcaccgtg ctgctcgtcg
     2581 tgctggtcac gaagttccag gccggcgcga agtacgccat cctcgctatg gccgtcctct
     2641 acgtcatgat gttggcgatc aaccgccact accggcacgt gagccaggag ctggccctgc
     2701 gcgatgacct gcgcgaggcg atcctgccca gccgggtgca tgcgctggtc ctggtctcca
     2761 agttgcacaa gccggccatg cgggccatcg cttatgcccg cgcgacccgt ccctcggtgc
     2821 ttgaggcgct caccgtcgaa gtcgacccgg aggagactgc ggcgttgggt cgcgagtgga
     2881 tcgctcgtga cgtcccggtg accctcaaga tcgtcgcgtc tccctaccgc gagatcaccc
     2941 ggccgatcat cgactacgtc aagtcgctac gcaccgacaa cccccgggac gtcgtcatcg
     3001 tctacgtgcc ggaatacgtc gtcggccact ggtgggagca actcctgcac aaccagagtg
     3061 cgctgcgtct gcgggtgcgt ctgcacttca tccctggggt catggtcgcc agcgtgccct
     3121 ggcagttgcg ctcgtccgag gggcagtcgg acgactggga cgaccgctcg gtccctggcc
     3181 gcgccggcaa catcgccccg tgagccccgt ctcccgttcg tccggccgac agcgccggta
     3241 tgccggtccg cgcaagcccg cgggtccccg cggagagtcg ctcgtcggcg aggtcttcga
     3301 ggtcgaggtc gggccggtcg cccatggtgg cttctgcgtc gcccggcacg acggccgcgt
     3361 cgtctttgtg cggcatacct tgccggggga gcgagtgcgc gcgaaggtga ccgaaggagg
     3421 cagcgaggac cgcttcctgc gggctgacgc cgtcgaggtc ctctccccgt cggcggaccg
     3481 tgtggatccg gtatgccgcc acgccggccc gggcgggtgc ggcggatgcg actggcagca
     3541 cgtcgacctg gccgcccagc gccggctcaa aggtgcagtc gtggctgaac aactgtggcg
     3601 gctcgccggc atcgaccgct cggtcgaggt gcttccggtg ccaggtgacg tggacggctt
     3661 cggctggcgc acgagggtgg agttcgcggt cgatcgggcc gggcgggcgg ggctgcggcc
     3721 gacccgctcg catgcggtcc tcccgatcat gcagtgcccg atcgccgacc cgcgggtgct
     3781 ggcgagcggg gtgctcgcgg acgactggtc cggtgcgaag gcggtcgacg tcgtcgcgcc
     3841 ctcaggtgga ggagccgtgg tcgtcgtgcc gattccggct gctgccggca gcgttcccga
     3901 gatccgcgaa cgagtcgaat ccgcctcgtg gacaggtgag ttcgacgtct cagcgcgtgg
     3961 cttctggcag gtgcacccgg gcgcggcggc cacctttgtc gaccgggtcc tcgccgagct
     4021 caacccgcgc ccgggggagc gggtgctcga cctgtatgcc ggtgtcggcc tgttcgctgt
     4081 gccgctggcc gatgcggtgg gcccgggcgg tgcggtgctg gccgtcgagg gggatgtcct
     4141 cgcctgcgag ttggccgtgc gtaatgcagg caaccgcacc tggattgagg tcgtccctga
     4201 ccgggttgag gaggtgctgg cctcaacgtc gccggacgcg tggtccagcg ccgatctggt
     4261 cgtcctcgac ccacctcgga cgggggctgg gcgcgcggtc atcgaggccg tggctcggtt
     4321 gcagccgcgg gccgtcgcct atgtcgcctg tgatccggcc gcactggctc gagatctggg
     4381 ctatgcgcgc gaggccggct acgtgttggg tgggctcacg gcatacgacg ccttcccgat
     4441 gacccaccac gtggagtgca tcgccgtgct gaagccaacc tgatccgacc ggcgatgacg
     4501 cgcggcgtgc gaactagcgc ccgtccgtgt cagaggccga aaagggtcag gagttcgcgc
     4561 ggtgtgagga cttcaatgcc gagattcttg gccgcatgca ggtcgtcgtc gccgctggtg
     4621 agggcgtccg ctgccgtgcc gagcgcaagt gcgacgaggt agtcgtcctt cgggtcgcgt
     4681 gtgaccgatg ggggattggc gatgtctgcg tgccactcgg cgagccggcg aatcagatcc
     4741 tcgaacgcgg cacctgcccc cggcttgacg tacctagata gcttcggccg agcgagcacg
     4801 tacacgacct catccaccag atgttgggtc acgaccggaa cgagcgttcc ttcgtcgatc
     4861 aatcggatga tgaggccgag ggcaccgttg ggcgtgatcg tggccgagat gaacacattg
     4921 gggtcgatga cgacccgacg cggtggagca ccg
//
LOCUS       DAOMZY010000271         4947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_16038_length_4947_cov_6.801717, whole genome shotgun sequence.
ACCESSION   DAOMZY010000271 DAOMZY010000000
VERSION     DAOMZY010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16038_length_4947_cov_6.801717"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1588)
                     /gene="purF"
                     /locus_tag="PLK64_13375"
     CDS             complement(<1..1588)
                     /gene="purF"
                     /locus_tag="PLK64_13375"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPV80781.1"
                     /translation="MAGGDGLLTHDLMPGEKGPQDACGVFGVWAPGEDVAKLAYFGLY
                     ALQHRGQESAGIATGDGHKVLVYKDMGLVSQVFNESALQSLTGHVAIGHCRYSTTGGS
                     TWENAQPTLGGTDTTTVALAHNGNLINSAELRDLVAERHGHLDRHPGELGRGNTTDTA
                     LVTALLSDPDRSLEAVALDVLPRLRGAFCFVFMDEHTLYAARDPHGIRPLALGRLDRG
                     WVVASETAALQTVGASVIREIEPGELVVIDEQGLRSHRFAEPEPKGCVFEYVYLARPD
                     AVINQRVVHEARVAMGARLAIEHPVDADLVIGVPDSGVPAAVGYAQASGIPFGQGFVK
                     NAYVGRTFIQPSQTLRQLGIRLKLNPLAHTIRGKRLVVVDDSIVRGNTQRAQVRMLRE
                     AGAAEVHVRISSPPVNWPCFYGIDFATRAELIATGLGVEEIRASIGADSLGYISLEGM
                     IEATNQPADRLCAACFTGTYPVDLPEDGRIGKHLFEPLPIDLSHSVRDVDPAPLSGAD
                     IGARAGGIRVDVEGVGLGVDG"
     gene            complement(1625..1882)
                     /locus_tag="PLK64_13380"
     CDS             complement(1625..1882)
                     /locus_tag="PLK64_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80782.1"
                     /translation="MTSPGTPPPSQRRRRAPRLVPFIATGAILGYVVGLLLAARPVAD
                     EAAQRLQNYSFNSQAGYLGLFGALMGALLGAVVFVVLDRRS"
     gene            complement(1879..2256)
                     /locus_tag="PLK64_13385"
     CDS             complement(1879..2256)
                     /locus_tag="PLK64_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol carrier family protein"
                     /protein_id="HPV80783.1"
                     /translation="MPPRRRIPPAEGLIALEVCRAAGFASAPREALATAVRFTLEELA
                     TLAPGRSVEVRVPPFGATQCVPGPVHRRGTPASVVETDAVTWLSVAVGDLGWAEALDS
                     GAVRASGERAALGEWLPLGRLSG"
     gene            2300..3346
                     /locus_tag="PLK64_13390"
     CDS             2300..3346
                     /locus_tag="PLK64_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HPV80784.1"
                     /translation="MRATTLHGAFDIRVSDLPDPKLHRPTDALVRVTASCVCGSDLWP
                     YRGINASKPGQRIGHEFVGIVEEVGSEVATVRPGDFVIAPFAWSDNTCRVCRVGIQTS
                     CENGGWWGGRDREGLPVDAGQGEFVRVPLADGTLVATPEVPSADLVPDLLTLSDVMGT
                     GWHCALSAGVRPGDTVAVVGDGAVGLCALLSARLQGATRIIAMSRHEARSAIAVEFGA
                     TEVVAERGEAGVARVRDLTDGFGVDAALECVGTAESMRTALDLVRPGGRIGFVGVPHD
                     VTVPIEDLFARNVHIGGGVAPVREYLPALRDLVLAGEIHPGRVFDLTVPLADVAEAYA
                     AMDQRRATKAMLLP"
     gene            3343..3774
                     /locus_tag="PLK64_13395"
     CDS             3343..3774
                     /locus_tag="PLK64_13395"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HPV80785.1"
                     /translation="MSNHGGEIAGTLIGLFWVLLGWWMIAVGRRRGRVGGDWVPAVGT
                     IVDKSGGTEGLFLRNPHIRYAAPDGSEHIVASRSHGDLWEPGQSVDILVDPTEPGRAA
                     LLTHAARATPYVVIGWFIIVVGLLTLIASLMLAVWVPPPST"
     gene            3845..4876
                     /locus_tag="PLK64_13400"
     CDS             3845..4876
                     /locus_tag="PLK64_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HPV80786.1"
                     /translation="MTGSAEGPSSESVRFEPDGHGGWTVMLDGQPQSHVDLADPGLIT
                     FEYVQHFALAIGACSPTAPAPLRVTHVGGAGLTLARWVQHTRPVSPQIVLEPDAALTA
                     AVRERLPLPRGHRIRVRPLDGRTGVRGLATASADVLVLDAYAAGRVPAELATPEFLAD
                     VARVLAPGGLLLANLADEPGLRWAARFLATLASVEGFGGPAVLAVLATHDVLKGRRFG
                     NLVAVASRGADDLANPAPTDAGPARSDSARLSPTDAEANRSGSARLSPTDAEANRPDS
                     SGLSRTGDGQPRLDLAALRRAGASAPIPTGIRSGPEVTRLIAGARPFADADAQPSPVP
                     PDPGAWRVR"
BASE COUNT          694 a         1813 c         1718 g          722 t
ORIGIN      
        1 caccgtcgac accgaggccc acgccttcga catcgactcg tatgccgccc gcgcgcgcgc
       61 cgatgtccgc ccccgacagg ggggccgggt cgacgtcccg caccgagtgg gagaggtcga
      121 tgggcaacgg ctcgaacagg tgcttgccga tccgaccgtc ctccggcagg tccaccgggt
      181 aggtgccggt gaaacacgcc gcgcacagcc ggtcagccgg ctggttcgtg gcctcgatca
      241 tcccctccag ggagatgtag cccaacgagt cggccccgat cgaggcccgg atctcctcga
      301 cccccaggcc agtggcgatg agctcggcgc gggtcgcgaa gtcgatgccg tagaagcacg
      361 gccagttgac cggcggcgag gagatccgca cgtggacctc ggccgccccc gcctcacgca
      421 gcatccggac ctgggcccgc tgggtgttgc cgcgcacgat cgagtcgtcg acgacgacca
      481 gccgcttgcc ccggatcgtg tgggccagcg ggttgagctt gagccggatg cccaactggc
      541 gcaacgtttg cgagggctgg atgaaggtcc gcccgacata ggcgttcttg acgaaaccct
      601 gcccgaacgg gatccccgac gcctgggcat aaccgacggc ggccggaaca ccggagtcgg
      661 gcaccccgat gaccagatcg gcatcgacgg gatgctcgat ggccaaccgc gcgcccatcg
      721 ccacgcgcgc ctcatgcacg acccgctgat tgatgacggc atcgggtcgg gcgaggtaga
      781 cgtattcgaa aacgcacccc ttgggctcag gctcggcgaa gcggtgcgac cgcagcccct
      841 gctcgtcgat gacgacgagc tcaccgggct cgatctcacg gatcaccgac gcgccgacag
      901 tctgcagggc agccgtctcc gacgccacga cccagccgcg gtcgagccga cccagcgcca
      961 gggggcggat gccgtggggg tcgcgggcgg catacagggt gtgctcgtcc atgaagacga
     1021 agcagaaggc gccccgcaac cgcgggagca cgtccaacgc cacagcttcg agcgagcggt
     1081 cggggtcgga gagcagtgcc gtcaccaggg ccgtgtcggt ggtgttgccg cgccccagct
     1141 ctccgggatg ccggtcgagg tgcccgtgcc gttccgcgac gaggtcacgc aactcggccg
     1201 agttgatgag gttgccgttg tgggccagcg caaccgtcgt cgtgtcggtg ccgccgagcg
     1261 tgggctgcgc gttctcccag gtcgacccgc cggtcgtcga gtagcggcag tggccgatcg
     1321 cgacgtgccc ggtgagcgac tgcagcgccg actcgttgaa gacctggctg accagcccca
     1381 tgtccttgta gacgagcacc ttgtggccgt cgccagtcgc gattccggcg gactcctggc
     1441 cgcggtgttg caacgcgtag agcccgaagt aggcgagctt ggccacgtcc tccccgggtg
     1501 cccagactcc gaagacccca caggcgtcct gtgggccctt ctcccccggc atgagatcgt
     1561 gcgttaacag cccgtcgcca cctgccacgt gcgcaagtgt cccacaggta tgccgtgctg
     1621 ccggtcagct cctgcggtcg agcaccacga acacgacggc ccccaacagc gcgcccatga
     1681 gcgcgccgaa cagcccgaga tacccggcct gcgagttgaa cgagtagttc tgcagtcgct
     1741 gggcggcctc gtctgccaca ggtcgggcgg ccaacagcag cccgacgaca tatccgagga
     1801 tggcgccggt cgcgatgaac ggcacgaggc gcggggcgcg acggcgtcgc tggctcggcg
     1861 gcggggtccc tggtgacgtc acccggacag cctacccagc ggcagccact cgcccagcgc
     1921 cgcccgctcc cccgacgcgc gcaccgcccc ggagtcgagc gcctcggccc aaccgaggtc
     1981 cccgaccgct acggacagcc aggtcacggc gtcggtctcg acaacggagg ccggggttcc
     2041 ccgccggtgg acgggccccg gcacgcactg ggtcgccccg aacggaggca cccgcacctc
     2101 gaccgatcgt ccgggcgcca gcgtggccaa ctcctcaagg gtgaaccgca cagcggtcgc
     2161 gagcgcctcc cggggggccg acgcgaaccc tgccgcacgg catacctcca gggcgatgag
     2221 gccctcggcg ggcgggatgc ggcggcgggg tggcaccccc acatggtgtc accgcgcgca
     2281 gacacgggag gatggagcca tgcgcgcgac caccctgcac ggggccttcg acatccgggt
     2341 gtccgacctg cccgacccca agctccaccg gccgaccgac gccctcgtgc gggtgacggc
     2401 ctcctgcgtg tgcgggtcgg acctgtggcc ctaccgcggg atcaacgcga gcaagccagg
     2461 gcagcggatc ggccacgagt tcgtcggcat cgtcgaggag gtcggctcgg aggtcgccac
     2521 tgtgcgcccc ggcgacttcg tcatcgcccc cttcgcgtgg tcggacaaca cctgtcgggt
     2581 atgccgtgtc ggcatccaga cctcgtgcga gaacggcggt tggtggggag gccgcgaccg
     2641 cgagggcttg ccggtcgacg ccgggcaggg cgagttcgtg cgggtgccgc tggccgatgg
     2701 caccctcgtt gcgacccccg aggtgccctc ggccgacctc gtgcccgacc tgctcaccct
     2761 ctcggacgtg atgggcactg gctggcactg cgcgctcagc gcgggcgtgc gaccgggcga
     2821 caccgtggcc gtcgtcggtg acggcgcggt cggattgtgc gcactgctgt cggcccggtt
     2881 gcagggcgcg acgcgaatca tcgcgatgtc gcgtcacgag gcccgttcgg cgatcgcggt
     2941 ggagtttggg gcgaccgagg tcgtggctga gcggggcgag gccggcgtgg ctcgggtccg
     3001 cgacctcacg gacggcttcg gtgtcgacgc ggccctggag tgtgtgggca ccgccgagtc
     3061 gatgcgcacc gccctggatc tcgtgcggcc tggtggccgg atcggcttcg tcggcgtccc
     3121 ccacgacgtc acggtgccga tcgaggacct cttcgcccgc aacgtccaca tcggcggtgg
     3181 cgtggcgccc gtgcgcgaat atctgcccgc gttgcgcgac ctcgtcctcg ctggcgagat
     3241 ccacccggga cgcgtcttcg accttacggt ccccctcgcg gacgtcgccg aggcgtatgc
     3301 cgcgatggac cagcgccgcg ccaccaaggc gatgctcctg ccatgagcaa ccacggaggc
     3361 gagatcgccg ggaccctcat cggcctgttc tgggtgctct tggggtggtg gatgatcgcg
     3421 gtcggtcgac gccgcggccg cgtcggcggc gattgggtgc cggcggtcgg gaccatcgtc
     3481 gacaagagcg ggggcaccga gggtctgttc ctgcgcaacc cgcacatccg gtatgccgcc
     3541 cccgacggct ccgagcacat cgtcgccagc cgctcgcacg gcgacctttg ggagcccggc
     3601 cagtcggtcg acatcctcgt cgaccccacc gaaccgggtc gcgcggcttt gctcacccac
     3661 gccgctcggg cgacgcccta cgtcgtcatc gggtggttca tcatcgtcgt cggtctgctc
     3721 acgctcatcg cgtcgctgat gctcgctgtc tgggtgccgc cgccatcgac gtgactcggc
     3781 tgactcggct gactcggctg actcggctga ctcggcggat gccgcaacgg cgaggcgcga
     3841 ccccgtgact ggatcagccg aagggccatc gtccgagtcg gtgcggttcg agccggacgg
     3901 ccacggcggg tggacggtca tgctcgacgg ccagccccag tcccatgtcg acctcgccga
     3961 ccccgggctg atcaccttcg aatacgtcca acacttcgcc ctcgccatcg gcgcgtgctc
     4021 gcccacggcc ccggcgccgc tgcgggtcac ccacgtcggt ggcgccgggc tcacgctggc
     4081 tcgctgggtg cagcacaccc ggccggtctc gccgcagatc gtcctggagc ccgacgccgc
     4141 actcaccgcg gccgtccgcg agcggttgcc gttgcctcgc gggcaccgga tccgggtgcg
     4201 tcccctcgac ggccgcaccg gcgttcgggg gctggcgaca gcgagcgccg acgtcctcgt
     4261 cctcgatgcg tatgccgcgg gccgagtccc ggccgagctc gccactccgg agttcctcgc
     4321 cgacgtggct cgagtgctgg cccctggtgg gttgctgctc gccaacctgg ccgacgagcc
     4381 cggattgcgt tgggcggcaa ggttcctcgc gacgctggcc tcagttgagg ggttcggtgg
     4441 gccagcggtg ttggcggtgt tggcgaccca cgacgtcctc aaggggcgcc gtttcggcaa
     4501 cctcgtcgcg gtggcatccc ggggagccga cgacctggcc aatcctgcac ccactgatgc
     4561 tggacccgct cgttcggact ccgcgaggct ctcacccacc gacgccgaag ccaatcgctc
     4621 gggctccgcg aggctctcac ccaccgacgc cgaagccaat cgaccagact cctccggcct
     4681 atcgcggacc ggggatggcc agccccgcct ggacctcgcc gcgctgcgtc gggccggtgc
     4741 gagcgcgccg atcccgaccg gcatacggtc cgggcctgag gtgacccgac tcatcgcagg
     4801 ggcgcgccca ttcgccgacg cggatgcgca accctcaccc gtcccgcccg accccggggc
     4861 ctggcgcgtc cgctgacgct gatccgcctc gcccgcgcct cccccctccg gccgggaaac
     4921 tcgacactcg gtgagttccc tccgagc
//
LOCUS       DAOMZY010000272         4940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_16069_length_4940_cov_5.025998, whole genome shotgun sequence.
ACCESSION   DAOMZY010000272 DAOMZY010000000
VERSION     DAOMZY010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16069_length_4940_cov_5.025998"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PLK64_13405"
     CDS             <1..577
                     /locus_tag="PLK64_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPV80787.1"
                     /translation="ALTGFHLHRGALAAMQRPVLPAVGGLLKNARRVLVLEDIVDHTN
                     VGACFRSAAALGVDAVLVTPRCADPLYRRAVRVSMGTVFQVPWTRIEPWPLGGAELLR
                     ENGFTVAAFALTETAMTLDELSADPPERLALVLGTEGDGLTRHTIAAADLAVRIPMAG
                     GVDSLNVAAACAVALWALRPSAIGASGAATV"
     gene            589..1530
                     /gene="rocF"
                     /locus_tag="PLK64_13410"
     CDS             589..1530
                     /gene="rocF"
                     /locus_tag="PLK64_13410"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HPV80788.1"
                     /translation="MSTPTKTVDLIGAPTDVGAAHRGASMGPEAMRVAGIQQTLAQFG
                     VSVNDLGNLAGPPNPWLPPVDGYRHLTEVQAWNQAVHDAVYGSLSGGRLPILLGGDHC
                     LAIGSISAVARHCRDQGLTLRVLWLDAHADFNTMGTTPTGNIHGMPVAILRGMGPVEL
                     TSIGGRTPAIDAGTVRQIGIRSVDVDEKRLVHEDGMEVFDMRNIDEHGMRKVMRKALE
                     GVDEGTHLHVSFDVDFLDPMIAPGVGTTVQGGPTYREAQLVMEMIADSGRLGSLDVME
                     LNPAKDHANRTAEVATDLIDSMFGKSTLLRLKGTPAG"
     gene            complement(1597..2787)
                     /locus_tag="PLK64_13415"
     CDS             complement(1597..2787)
                     /locus_tag="PLK64_13415"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HPV80789.1"
                     /translation="MSALVLVLNAGSSSLKYQLLDADSGTVSASGVVERIGEPDAKAV
                     ARHTVSGEATTRDVDAPDHHAAMRVVLDAFATDGPPLRPQDLYAVGHRVVHGGDLFRS
                     PAIVDDAVIDAIEDLVPLAPLHNPGNLAGIRAARAAFPTTPQVAVFDTAFHQTLPPHA
                     FTYAVPREWRERHRVRRYGFHGTSHAFVSRRLATLLGRDVSETNVVVLHLGNGASAAA
                     VAGGACIDTSMGLSPLEGLVMGTRPGDLDPALPSHLAREGVPIEDYDRALNAGSGLRG
                     LTGTNDFREVERLIEAGDPDAALALDIVTYRIAKYVGSYAVALGRLDGIAFTAGIGEH
                     SPRLRAEVLARLGMLGIRVDPDRNSGGPAERVVTSDDSAVPAWVVPTNEELEIARQTL
                     AVLR"
     gene            complement(2784..>3053)
                     /locus_tag="PLK64_13420"
     CDS             complement(2784..>3053)
                     /locus_tag="PLK64_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HPV80790.1"
                     /translation="IQYDAAVDPAVAATKATGSPVAGRATVLIFPDLNTGNNTYKAVQ
                     RSAGAVAIGPILQGLRQPVNDLSRGALVQDIVNTVAITAIQAVGR"
     assembly_gap    3054..3153
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3154..4631)
                     /locus_tag="PLK64_13425"
     CDS             complement(<3154..4631)
                     /locus_tag="PLK64_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HPV80791.1"
                     /translation="MASSIYLAGTGRDSGKSAIALGLLDQAIRRLPSVAVFRPLVRSG
                     DSSLGTTLDLLLSRLPAPPPRQDCIGVTYQQMHDDPDRAMATIVERYHQLAAGHQAVL
                     IVGSDFTDVGAPTEFSVNARIAANLGAPMLLVASGLGLTPAEVRGAVELAIDDLHDNH
                     ATVLGVVANRVSPEHLEATRTALAGVAGTHPVYAVPAHPLLSAPSVRDQMTAAGGRLL
                     YGEDSLLDREATGLLIGAMTLPNILGRLSEGAVLITPGDRSDVLLGVLLAHHSRTFPS
                     LAGIVLNGGLPIDPQIHTIVEGLKLSLPVIEVGLGTSDTARVLGAVTGRITRTSERKI
                     QTAIALVDEVIDGTKLLREIQIAPTEVVTPVMFEHRLIDRARRADRHIVLPEGEEDRI
                     LIAADQLLRRKVARLTLLGDADLIRSKAQRLQLDLAGADILNPATDPLRERFAHEMVE
                     LRKAKGVPYDLAWDSARDVSYFGTMMVHDGLADGMVSGSVNTT"
     gene            4799..4882
                     /locus_tag="PLK64_13430"
     tRNA            4799..4882
                     /locus_tag="PLK64_13430"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4832..4834,aa:Leu,seq:gag)
BASE COUNT          717 a         1552 c         1861 g          710 t
ORIGIN      
        1 ggcgctgacg ggcttccacc tgcaccgcgg cgcgctggcg gcgatgcagc gccccgttct
       61 ccccgcggtg ggcggcctgc tcaagaacgc ccggcgagtg ctcgtgctcg aagacatcgt
      121 cgaccacacc aacgtcgggg cttgtttccg aagcgccgca gccctcggcg tcgatgccgt
      181 ccttgtcacc ccgcgctgcg cggacccgct ctatcgccgc gccgtgcgag tgtcgatggg
      241 cacggtcttc caggtgccct ggacgcggat cgagccgtgg ccgctcggcg gtgcggaact
      301 cttgcgagag aacggtttca ccgtcgccgc cttcgcgctc accgagaccg ccatgaccct
      361 cgacgagctc tcggccgacc cgccggagcg gctcgcgctg gttctgggga cggaagggga
      421 tgggctgaca cggcatacca tcgcggcggc cgacctcgcg gtccgcatcc cgatggctgg
      481 gggagtggac tccctcaacg tcgcggcggc ctgcgcggtg gcgctctggg cgctgcgccc
      541 gagcgcgata ggtgccagcg gggccgcgac tgtgtgagga tgctcgccgt gagcaccccc
      601 acgaagacag tcgatctcat cggagcccct acagacgtcg gcgcggcaca ccgcggagcg
      661 tcgatgggcc cagaagccat gcgcgtcgcc ggaatccagc agacgctcgc ccagttcggg
      721 gtgagcgtca acgacctcgg caacctcgcc ggaccgccca acccctggct gccgccggtg
      781 gatggttacc ggcacttgac cgaggtgcag gcgtggaacc aggcggtcca cgacgcggtc
      841 tacgggtcgc tgtccggcgg gcggctgccg atcctcctgg gcggcgacca ctgcctggcg
      901 atcggctcga tcagcgctgt cgcgcgccac tgccgcgacc agggcctcac gctgcgcgtg
      961 ctgtggctcg acgcgcacgc cgacttcaac actatgggca ccaccccgac cggaaacatc
     1021 cacggtatgc cggtcgcgat ccttcgcggc atgggcccgg tcgagctgac ctccatcggc
     1081 ggtcggacgc cggcgatcga cgcagggacg gtgcggcaga tcgggattcg cagcgtcgac
     1141 gtcgacgaga agcggctcgt gcatgaggac ggcatggagg tcttcgacat gcgcaacatc
     1201 gacgagcacg ggatgcgcaa ggtcatgcgc aaggccctgg agggcgtcga tgagggcacc
     1261 cacctgcacg tgagcttcga tgtcgacttc ctcgacccga tgatcgcgcc cggggtcggg
     1321 acgacggtgc agggcggccc gacctatcgg gaggcccagt tggtcatgga gatgatcgcg
     1381 gacagcggcc ggctcggctc gctcgacgtc atggagctca acccggccaa ggaccacgcc
     1441 aaccggaccg ccgaggtggc gacagacctc atcgactcga tgttcggcaa gagcacgctg
     1501 ctgcgcctca agggcacacc cgcgggctga tctggacgtg aacgcgcggg cccatcgggg
     1561 cccgcgcgac cgtcgcgttc agcgcgggac ggcgtgtcag cgcaggacgg cgagagtctg
     1621 ccgggcgatc tccaactcct cgttggtggg gaccacccac gcaggcacgg cgctgtcgtc
     1681 gctcgtcacc acgcgttccg ccggcccgcc ggagttccgg tccggatcga cacggatccc
     1741 cagcatcccg agccgagcca acacttcggc ccgcagccgc ggggagtgct caccgatgcc
     1801 agccgtgaag gcgatgccgt cgagccgacc cagcgcgacg gcatacgagc cgacgtattt
     1861 cgcgatgcgg taggtgacga tgtcgagcgc gagggcggcg tcagggtcgc ccgcttcgat
     1921 gagccgctcc acctcgcgga agtcgttggt gccggtgagg ccgcgcagcc cgctgcccgc
     1981 gttgagcgcc cggtcgtagt cctcgatcgg gacgccctcg cgcgcgaggt ggctcggcag
     2041 ggcggggtcg aggtcgcccg gccgggtccc catgacgagg ccctccagcg ggctgagccc
     2101 catcgacgtg tcgatgcagg cccctccggc gacggcagcc gcgctggccc cattgcccag
     2161 gtgcaggacg acgacgttgg tctcgctcac gtcgcggccc agcagggttg ccagccggcg
     2221 cgagacgaag gcgtgcgacg tgccgtggaa gccgtagcga cggactcggt gccgctcgcg
     2281 ccattcgcgg ggcacggcat acgtaaaggc gtgcggcggc agggtctggt ggaaggcggt
     2341 gtcgaagacg gcgacctggg gggtcgtagg gaaggcggcg cgggcggcgc ggatccccgc
     2401 gaggttgccg ggattgtgca gcggcgcgag gggcacgagg tcctcgatcg cgtcgatgac
     2461 ggcgtcgtcg acgatcgcgg gggagcggaa gaggtcgccg ccgtggacga cccggtggcc
     2521 cacggcatac aggtcctgcg ggcgcagggg cgggccgtcg gtggcgaagg cgtcgaggac
     2581 gacgcgcatt gccgcgtggt ggtcgggggc gtcgacatcg cgtgtcgttg cctcgcccga
     2641 gacagtatgc cgtgcaacgg ctttcgcgtc cggctcaccg atgcgttcga caacaccgga
     2701 ggcggagacc gtcccagagt cggcgtcaag cagctggtat ttgagagagg acgagccagc
     2761 gttgaggacc aggacgaggg cgctcatcgg ccgactgcct ggatcgccgt gatggcgacg
     2821 gtgttgacga tgtcctgcac gagcgcgccg cgggagaggt cgttgaccgg ctggcgcagg
     2881 ccttggagga tcgggccgat cgcgaccgcg ccggcgctgc gctggacggc cttgtaggtg
     2941 ttgttgccgg tgttgaggtc cgggaagatc agcaccgttg cgcgaccggc gacgggggag
     3001 ccggtggcct tggtggccgc gacggccggg tcgacggcgg cgtcgtattg gatnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtcgtgt tgaccgagcc cgacaccatg
     3181 ccgtcggcga ggccgtcgtg gaccatcatc gtgccgaagt aggacacgtc acgggcggag
     3241 tcccaggcca ggtcgtaagg gacgcccttg gccttgcgca gctcgaccat ctcgtgggcg
     3301 aaccgttcgc gcagcgggtc ggtggccggg ttgaggatgt cggcgccggc gaggtccaac
     3361 tgcaggcgct gggccttgga gcggatgagg tcagcgtcgc cgagcagggt cagccgggcg
     3421 accttgcggc gcagcagttg gtcggcggcg atgaggatcc ggtcctcctc gccctcgggc
     3481 aggacgatgt gccggtcggc tcgccgggcc cgatcgatga gccggtgctc gaacatcacc
     3541 ggggtgacga cctcggtcgg ggcgatctgg atctcccgca acagtttggt gccgtcgatg
     3601 acctcgtcga ccagggcgat ggcggtctgg atcttgcgct cggacgtgcg ggtgatccgg
     3661 ccggtgacgg cgccgaggac ccgcgcggtg tcggaggtgc cgaggccgac ctcgatgacc
     3721 ggcagcgaca gtttgaggcc ctcgacgatc gtgtggatct gcgggtcgat cggcaggccg
     3781 ccgttgagca cgatgccggc cagcgacggg aaggtgcgcg agtggtgagc caggagcacc
     3841 ccgaggagca cgtcggaccg gtcgccggga gtgatgagga cggcgccttc ggacagtcgc
     3901 ccgaggatgt tgggcagggt catcgcgccg atcaacaggc cggtggcctc gcggtcgagc
     3961 agtgagtcct cgccataaag gagccgtccg cctgccgcgg tcatctggtc acggacgctc
     4021 ggcgcggaca gcagcgggtg ggcgggcacg gcatacaccg ggtgggtgcc ggccaccccg
     4081 gcgagggcgg tgcgggttgc ctcaaggtgc tcgggggaga cgcggttggc gacgacgccg
     4141 aggacggtgg cgtggttgtc gtggaggtcg tcgatggcga gttccacggc gccgcggacc
     4201 tcggcggggg tgaggccgag cccagacgcg acgaggagca tgggagctcc gaggttggcg
     4261 gcgatccggg cgttgaccga gaactccgtg ggcgcgccga catcggtgaa gtccgagccg
     4321 acgatgagga cggcctggtg gccggcggcc aactggtggt agcgctcgac aatcgtcgcc
     4381 atcgcgcggt cggggtcgtc gtgcatctgc tggtaggtga cgccgatgca atcctgccgc
     4441 ggcgggggag cgggcagccg cgagagcagg agatcgaggg tcgtgcccag ggaggagtcg
     4501 ccggagcgga cgagcggccg gaagaccgcc acgctaggca ggcgccggat ggcctggtcg
     4561 agcagtccaa gggcgatcgc cgacttgccg ctgtcgcggc cggtgcccgc gaggtagatg
     4621 ctggaggcca cgggcacagg ctagtgggat gggggtcgag ggcgcgtggg tgaccaggga
     4681 cggtgctcgt tccgggccag tcccggcctg gctgagtcgg gttcgaggta tgccgccctc
     4741 ccgtgctaac gttgccggtc gcgaggcacc ccgaggtttg gggccgccga gcacacctgt
     4801 ccgggtggcg gaatggcaga cgcgctagct tgaggtgcta gtgcccttaa cgggcgtggg
     4861 ggttcaagtc cccctccgga cacagatgtg atgtctcaag acatcccgga cagggcgaac
     4921 cctcagggtt cgccctgtct
//
LOCUS       DAOMZY010000273         4933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_16098_length_4933_cov_6.011070, whole genome shotgun sequence.
ACCESSION   DAOMZY010000273 DAOMZY010000000
VERSION     DAOMZY010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16098_length_4933_cov_6.011070"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PLK64_13435"
     CDS             complement(<1..474)
                     /locus_tag="PLK64_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HPV80792.1"
                     /translation="MVMPNIGAFIAWGLITAIFIKSGWLNSGGSGGLAANSWVAQIGG
                     WGDFDGKGIVGPMITYLLPILIGYTGGKIIYDVRGGVVGAIATMGVIAGTSIPMFMGA
                     MVMGPLGGWSIKKLDALWDGKIRPGFEMLVNNFSAGIWGMVLAVFGFFAMGPLIIT"
     gene            721..1779
                     /locus_tag="PLK64_13440"
     CDS             721..1779
                     /locus_tag="PLK64_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent dehydrogenase"
                     /protein_id="HPV80793.1"
                     /translation="MKALRFYAPDDLRLEDVPEPECGPDEVKMRVRNCSTCGTDVKIR
                     HNGHQNLTPPRIIGHEIAGEIVEVGAEVNASYGTQWQVGDRVQVIAAVPCGDCYECSK
                     GWMEVCQNQTSVGYQYDGGFAEFMIVPRQVLKVDGLNRIPDNVGFDEASAAEPFACAI
                     NAQEQLGIEPGDTVVVFGAGPIGCMHIRIARGVHQCGPVYLVDVNAERLAMSADAVHP
                     DEVINAADGDVVARVLELTNGRGADVIITATAANITQEQAIAMAARNGRISFFGGLPK
                     TNPTITCDSNLVHYRQLHIHGANGSAPSHNKKALEYISTGQVPVKDLITRHVALDDVL
                     SAFDIVARGEAIKVTVEP"
     gene            1936..2697
                     /locus_tag="PLK64_13445"
     CDS             1936..2697
                     /locus_tag="PLK64_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HPV80794.1"
                     /translation="MYAPERQQAILATARADGRVEVAALAETLKVTPETVRRDLTALE
                     RLGVLRRVHGGALPVERLEVEPTLATRRDQFAEEKLRIAARALDELPREGTVLLDSGS
                     TTLALAEMFPRDRELTVVTNSIAIASALHGHHGVDLYVLGGRIRTRTGAAVGSWATTA
                     LAGVSVDVAILGTNGFSVRRGMTTPDQAEAVAKRAMVAAGRRCVVLADSSKAELVHFH
                     RFAELDDVALLITDRRLDEETAERLEASGTDVVRA"
     gene            2829..3779
                     /gene="pfkB"
                     /locus_tag="PLK64_13450"
     CDS             2829..3779
                     /gene="pfkB"
                     /locus_tag="PLK64_13450"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009380977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HPV80795.1"
                     /translation="MIVTLTPNPSIDRTVSVPALEHGEVNRASDSRIDPGGKGINVAR
                     ALATNGTEALAVLPSGGPEGHLLQALLRRSGTAYTAVPIAGTTRMNIAVIEPDGTTTK
                     LNEPGPTLSADEFDALLATVDNHLDPDLRWIVGCGSLTPGMPEGIYADLVRRGHAHGI
                     KVAIDSSGAPFTAAVPAGPDLIKPNHEELEELVGRPLHTLAEVGEAARTLVDGGIETV
                     IVSLGKHGALLVNAVAVAHAIAHVAHPVSTVGAGDSLVAGYLHAVCRGAGPREALATG
                     VAWGAAAVSLPGSRMPGPDDIAAITVTSTDTPDLTLSLTD"
     gene            3828..4277
                     /locus_tag="PLK64_13455"
     CDS             3828..4277
                     /locus_tag="PLK64_13455"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HPV80796.1"
                     /translation="MSLISESQVILDLDYPDRHQATRALAETLVADGRCTDLDLFLSD
                     VRKREEQMATGLPGGIGIPHARSGGITTPSLVFGRSRNGIDWGAEDGPATLIFLIAAP
                     DGGGDAHLAILAKLARKLMRKDFRQSLSDAATAAEVVEIVEREVVNA"
     gene            4274..>4933
                     /locus_tag="PLK64_13460"
     CDS             4274..>4933
                     /locus_tag="PLK64_13460"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIB"
                     /protein_id="HPV80797.1"
                     /translation="MKFVGVTSCPTGIAHTYMAAESLEQAAKDAGHEMIVETQGSAGS
                     DPIPQSVIDAADAVVFAHDLPVKDAARFAGKPTIDVGVKRAISDGPGLIAQAAAAAAD
                     FKANPAKAAAAAAAAPTGGLATKVDSSASLGTQVRQWLMTGVSYMIPFVAAGGILIAL
                     GFMLATFALGKDGPVLITAYDINLPKDVAHSIMDSFNPLSLTWWAALLFRVGVAAFGF
                     LV"
BASE COUNT          831 a         1812 c         1533 g          757 t
ORIGIN      
        1 cgtgatgatg agtgggccca tggcgaagaa gccgaagacc gcgaggacca tcccccagat
       61 gcccgccgag aagttgttga cgagcatctc gaagcccggc cggatcttgc cgtcccacag
      121 ggcgtcaagc tttttgatcg accagccacc gagcggaccc atgaccatgg cacccatgaa
      181 catcgggatg ctcgtcccgg cgatgacacc cattgtcgcg atggcaccga cgacgccccc
      241 gcggacgtcg tagatgatct tgccgccggt gtagccgatg aggatcggca acaggtaggt
      301 gatcatcggg ccgacgatgc ccttgccgtc gaagtcgccc cacccgccga tctgggcgac
      361 ccagctgttg gcggcgagcc cgccgctgcc accggagttg agccacccgg acttgatgaa
      421 tatcgcagtg atgagtcccc aggcgatgaa cgcaccgatg ttgggcatga ccatgttgga
      481 caggaacgtc ccgaacttct gaacgtggac acgggccgac gttcgttggg cctcggtggt
      541 ctgcgttgac gacacctttg ccagctccct tcgctgtgac ccacgacata ccgcgggtct
      601 gtgtttgcct ttatataggc ccgttcttga ccgtgtcaag gttccatgcc atgattcagg
      661 cacaaacaca catgattggg gtcgcggagc ggcccgatta gccccaatgg aggagcatcg
      721 atgaaggccc tgcgcttcta cgcccccgac gacctgcgcc tggaggacgt ccccgagccc
      781 gagtgtggcc cggacgaggt caagatgcgg gtccgcaact gctccacctg tggcacggac
      841 gtgaagatcc gccacaacgg gcaccagaac ctcaccccgc cgcgcatcat cggtcacgag
      901 atcgccggcg agatcgtcga ggtcggcgcc gaggtcaacg cgagttatgg cacgcagtgg
      961 caggtcggcg accgggtcca ggtcatcgcc gcggtgccgt gcggcgactg ctacgagtgt
     1021 tcgaagggtt ggatggaggt ctgccagaac cagacgtccg tcgggtacca gtacgacggc
     1081 ggcttcgcgg agttcatgat cgtcccccgc caggtcctca aggtcgacgg actcaaccgg
     1141 atcccggaca acgtcggctt cgacgaagcc tcggccgccg agccgttcgc ttgcgcgatc
     1201 aacgcccagg agcagttggg catcgagccc ggcgacaccg tcgtggtctt cggcgccggc
     1261 ccgatcggct gcatgcacat ccggatcgcc cgcggcgtgc accagtgcgg gccggtctat
     1321 ctcgtcgatg tcaacgccga gcgcctggcc atgtcggccg acgccgtcca ccccgacgag
     1381 gtcatcaacg cggccgacgg cgatgtcgtg gcccgcgtgt tggagctgac caacggccgc
     1441 ggcgcggacg tcatcatcac ggcgacggcc gccaacatca cccaggagca ggcgatcgcc
     1501 atggctgctc gcaacgggcg gatctcgttc ttcggtgggc tgcccaagac caacccgacg
     1561 atcacctgcg actcgaacct cgttcactac cgccagttgc acatccacgg cgccaacggt
     1621 tcggcgccca gccacaacaa gaaggccctg gagtacatct cgaccggcca ggtccccgtc
     1681 aaggacctca tcacccggca tgtcgccctc gacgacgtcc tgagtgcctt tgacatcgtc
     1741 gcgcgcggcg aggcgatcaa ggtcacggtc gagccctgac ccggaacggc cgggggtcgg
     1801 ccccacgaag gcagatcccc ggctagatcc gatcgggccg gttcgggcag aatcccacac
     1861 atggcttgtc cggaggaccg atcaccgacc ccaggctcgg caacggcgcc gagcccagac
     1921 gaggagcgtg ccccgttgta tgccccagaa cgtcaacagg ccatcctggc cacggcgaga
     1981 gccgacggcc gagtggaggt cgcggccctg gccgagaccc tcaaggtcac gcccgagacc
     2041 gtgcgccgcg acctcacggc gctcgagcgt ctcggcgttc tgcgccgcgt ccatggtgga
     2101 gccctgcccg tcgaacggct cgaagtcgaa ccaacgctgg ccacccgacg cgaccagttc
     2161 gccgaggaga aactgcggat cgccgcccgc gccctggacg agctcccgcg cgaaggcact
     2221 gtcctgctcg actcgggatc gacgacgctg gccctcgccg agatgtttcc gcgtgatcgc
     2281 gagttgacgg tcgtcaccaa ctcgatcgcc atcgcctcgg ccctgcacgg ccatcacggc
     2341 gtggacctct atgtcttggg aggccggatc cgcacgcgca cgggcgcggc ggtcggctcc
     2401 tgggccacga cggccctcgc cggcgtcagc gtcgacgtcg cgattctcgg gaccaatggc
     2461 ttctcggtcc ggcgcgggat gaccactccc gaccaggccg aggccgtggc caagcgcgcc
     2521 atggtcgcgg ccgggcggcg atgtgtcgtg ctcgccgact cgtccaaggc cgaactcgtc
     2581 cacttccacc ggttcgccga gctcgacgat gtcgcgctgc tcatcaccga ccgtcgcctg
     2641 gacgaggaga ccgccgagcg gctcgaggcc agcgggacgg acgtggtccg ggcctgaacg
     2701 gcgccaggca cgctcagcaa ccacgctggc gagcaactcg gacacaaaat ccggctcaag
     2761 actatttaca tcgctttgtt tgtgtgggaa tatttgtgca agcgcttgcg caccctcagg
     2821 aggccaggat gatcgtcacc ctcaccccca acccgagcat cgaccgcacc gtctcggtgc
     2881 cggctctcga gcacggcgaa gtcaaccggg cgtccgacag ccggatcgac ccgggcggca
     2941 agggcatcaa cgtcgcccgc gcgctggcca ccaacggcac cgaggccctc gccgtgcttc
     3001 cctccggcgg acccgagggg cacctgctgc aggccctcct gcgccgctcg ggcacggcat
     3061 acaccgcggt ccccatcgcc gggacgactc ggatgaacat cgcggtcatc gaacccgacg
     3121 gcacgaccac caagctcaac gagcccggtc cgaccctctc cgccgacgag ttcgacgccc
     3181 tcctcgcgac ggtcgacaac cacctcgacc ctgacctgcg ctggatcgtc ggctgcggca
     3241 gcctcacccc ggggatgccc gaaggcatct atgccgacct cgtccggcgc ggccacgcgc
     3301 atggcatcaa ggtcgccatc gactcctccg gggcgccgtt caccgccgcc gtgcccgccg
     3361 gccccgacct catcaagccc aaccacgagg agctcgaaga gctcgtcggt cgcccgctgc
     3421 acaccctcgc cgaggtcggt gaggccgcgc gcacgctggt cgacggcggc atcgagacgg
     3481 tcatcgtcag cctcggtaag cacggcgctt tgctggtcaa cgccgtcgcc gtcgcccacg
     3541 cgatcgcgca cgtcgcccac ccggtgtcga ccgtcggcgc tggagactcg ctcgtggccg
     3601 gctacctcca cgcggtatgc cgcggcgccg gcccccgtga agcgctcgcc accggggtcg
     3661 cctggggcgc cgctgccgtc agtcttcccg gcagccggat gcctggcccg gacgacatcg
     3721 ccgccattac ggtgaccagc accgatactc ccgacctcac cttgtcgctc accgattgac
     3781 cccagttttg ccgagcccga cgattcacat ctaaggagtc cctcaccatg tccctcatca
     3841 gcgagagcca ggtcatcctc gacctggact atcccgaccg gcaccaggcg acgcgcgccc
     3901 tggccgagac cctcgtggcc gacggccgat gcaccgacct cgatctgttc ctctccgacg
     3961 tccgcaagcg tgaagagcag atggcgaccg ggctgcccgg cggcatcggg atcccgcacg
     4021 cccgctcagg cgggatcacc accccgtcgt tggtcttcgg gcgctcgcgc aacggcatcg
     4081 actggggcgc cgaggacggc ccggccaccc tcatcttcct catcgctgca cccgatggcg
     4141 gtggggatgc gcacttggcc atcctcgcca agctcgcgcg caagctcatg cgtaaggatt
     4201 tccgccagtc cctgagcgac gcagccaccg cggccgaggt cgtcgagatc gtcgaacgag
     4261 aggttgtgaa cgcatgaagt tcgtcggagt gacctcgtgc cccacgggca tcgcacacac
     4321 ctacatggcc gccgagtcct tggagcaggc agccaaggac gccggccacg agatgatcgt
     4381 cgagacccaa ggctcggccg gatctgaccc gatcccccag tcggtcatcg acgccgctga
     4441 cgccgtggtc ttcgcccacg acctcccggt caaggacgct gcccgcttcg ccggcaagcc
     4501 gaccatcgac gtgggcgtca agcgcgcgat cagcgacggc ccgggcctca tcgcccaagc
     4561 cgcggctgcc gccgcggact tcaaggccaa ccccgccaag gccgctgccg cagccgccgc
     4621 ggccccgacc ggtggcctcg cgaccaaggt cgactcgtcg gccagcctcg gcacccaggt
     4681 ccgccagtgg ctcatgaccg gtgtctccta catgatcccg ttcgtcgccg ccggtggcat
     4741 cctcatcgcc cttggcttca tgctcgcgac cttcgccctc ggcaaggacg gccctgtcct
     4801 catcacggca tacgacatca acctgcccaa ggatgtcgcg cacagcatca tggacagctt
     4861 caacccgctg agcctcacct ggtgggcggc gctgctcttc cgcgtcggtg ttgcggcctt
     4921 cggcttcctc gtc
//
LOCUS       DAOMZY010000274         4901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_16263_length_4901_cov_4.726991, whole genome shotgun sequence.
ACCESSION   DAOMZY010000274 DAOMZY010000000
VERSION     DAOMZY010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16263_length_4901_cov_4.726991"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PLK64_13465"
     CDS             complement(<1..650)
                     /locus_tag="PLK64_13465"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008693.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HPV80798.1"
                     /translation="MTAPLFLVAPGSLAGAMAGTTVSIGGSEGHHAVSVHRLRIGEEV
                     LVGDGAGVRVSAEVVGVERHTLLTRVIEVQVRPPADPRFVLVQALAKGDRDEQAIEAA
                     TELGVDAVVPWQASRSVVQWRADKVARGQARWESIVVAAAKQSRRFQVPQVRPLARTA
                     DVVRLLGDAALGIVLHEEAQAPLASLDLPPTGEIVLVVGPEGGIAQTELDAFAAVGG"
     gene            complement(694..1818)
                     /gene="dnaJ"
                     /locus_tag="PLK64_13470"
     CDS             complement(694..1818)
                     /gene="dnaJ"
                     /locus_tag="PLK64_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPV80799.1"
                     /translation="MSDYYQDLGVERSATAEEIKRAYRRLARQLHPDVNSGAEAEEQF
                     KTVSRAYEVLSDPDKRRNYDLGVDPFAAGGGAGFSGQGFSFSDVMDAFFGGAATQRGP
                     RSRAHRGQDALVRIQIDLHDAVFGSEEELTIETAVRCSSCQGDGCQPGTGVSTCVLCH
                     GRGEVQTVQRSFLGQVMTTRPCQACQGYGSTINHPCFECSGEGRVRTRRTLTLKVPAG
                     VDSGTRIQLAGEGEVGTGGGSAGDLYVEVAVANHATYTRRGDDLHCRVELPMTAAALG
                     TTIDLQTFDGTRPFDVRPGTQAGDTMTLRGLGVTHLRGTGRGDLIVHADVQTPTRLTE
                     GQQDLLRQLAQLRGEERPIGRMAPADKGLLGKLRDAFKNA"
     gene            complement(1854..2888)
                     /gene="hrcA"
                     /locus_tag="PLK64_13475"
     CDS             complement(1854..2888)
                     /gene="hrcA"
                     /locus_tag="PLK64_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HPV80800.1"
                     /translation="MSDERRLSVLRAIVQDYVRTSEPVGSKSLVERHHFGVSAATVRN
                     DMAILEEEGLIHAPHTSAGRVPTDAGYRVFVDRLSAVKPMSGAERSAISHFLQGAVDL
                     DDVVDRTVRLLASLTHQVAVVQYPSLTRSAVRHIELVPMARDRLMVILIANTGRIEQR
                     VVDAHCRLDDETGAALVSRIRSEVLGEAAGRSFPDAAARLQSLPERFEPADRDLVRIV
                     LRTLDDALAEEREERVVLAGTSNLARFGADFPLTIAPVLEALEEHVVLLKLFGQLRQG
                     QGGAVAVTIGHEHEHQGLAGASVVSAAYGTGAELVAGLGILGPTRMDYPNAMATVRAV
                     AHYVSELLEP"
     gene            3032..3910
                     /locus_tag="PLK64_13480"
     CDS             3032..3910
                     /locus_tag="PLK64_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3097 domain-containing protein"
                     /protein_id="HPV80801.1"
                     /translation="MDRYGRDVLADAARMPRRPVAAPVAAEPGLVVEDVETGWCGAVV
                     RVEKAGGMHVVHLEDRRGRTKGFPLGPGFLLEGRPVVLTPPVAADRAKLEAVRKAAAR
                     TASGSVAVVGQPARVAVGSRIWVEGRHDAELVEKVWGADLRVEGVVVEQLDGIDDLVA
                     SVRSFAPGPGRRLGILVDHLVPGTKESAIAAAVRALPAATQGHALLLGHPYVDVWQSV
                     KPARLGWTQWPVIPRGRSWKEGICQALGWPCDTPADLAFAWKRILGTVGSYADLEPTL
                     LAAVEELIDFVTAPGA"
     gene            3939..4478
                     /locus_tag="PLK64_13485"
     CDS             3939..4478
                     /locus_tag="PLK64_13485"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HPV80802.1"
                     /translation="MSDQPANDPTRPVDVPPPPPPAWSGSAPEAPAAPVPAPPAYQAP
                     PAAYQTPAAPPPPAYPPQGYAAPVAGVVPLSPAESRQWGMLSHVGGIILGFVGPLIVM
                     LVFGPRDAFTKDQSTEALNFQLTLLIGYIAGTILTFILPLPVGLIVWVLGLVFAIMGA
                     LAANKGEAYRYPFSLRLVK"
     gene            complement(4520..>4901)
                     /locus_tag="PLK64_13490"
     CDS             complement(4520..>4901)
                     /locus_tag="PLK64_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80803.1"
                     /translation="AGPGAHSHVGGVRWWNVKHPRAYAARLGDAGIGAPRGSAALGPV
                     LSPAAAREVLTSEQQADERVLLGVRLVDGLPIADLAAGAAREVAGLVAEGLVDGRAAL
                     AERRVRLTLTGRLLADTVVRRLVG"
BASE COUNT          684 a         1806 c         1692 g          719 t
ORIGIN      
        1 ccgcctaccg ccgcgaaggc atccaactcg gtctgtgcga tgccgccctc ggggccgacg
       61 acgagcacga tctccccggt cggcggcagg tcgaggctcg ccaacggcgc ctgtgcctcc
      121 tcgtgcagga cgatgccgag agcggcgtcg cccagcagcc gcaccacatc ggcggtgcgc
      181 gccagaggcc tgacctgggg gacctggaac cggcgcgact gcttcgccgc cgccaccacg
      241 atcgactccc aacgcgcctg gccacgggcg actttgtcgg cgcgccactg aacgacgctg
      301 cgggacgcct gccacgggac gacggcatcg acgccgagct cggtcgcggc ctcgatcgcc
      361 tgctcgtccc ggtcaccctt ggccagcgcc tggaccagga cgaacctcgg gtccgcgggc
      421 gggcgcacct gcacctcgat gaccctggtg aggagggtat gccgttcgac gccgaccacc
      481 tcggcgctca cccggacccc ggccccgtca ccgacaagca cctcttcccc gatgcgcaac
      541 cggtggaccg agaccgcgtg gtggccttcc gagccgccga tcgacaccgt ggtgcccgcc
      601 atcgccccgg ccagagagcc aggtgcgacg aggaacagcg gggcggtcac ggcataccgg
      661 cttcgtcagg cgttggtcgc tgggagttcg tcgtcaggcg ttcttgaagg cgtcgcgcaa
      721 cttgccgagc aggcccttgt cggcaggcgc catccgcccg atcggacgtt cctcgccacg
      781 caactgggcc aactgacgca acaggtcctg ctggccctcg gtgagccggg tcggagtctg
      841 gacgtcggca tggacgatga ggtcgccgcg gccggtgccg cgcaggtggg tcaccccaag
      901 gccacgcagc gtcatcgtgt cgcccgcctg ggtgcctggc cgcacgtcga aggggcgggt
      961 gccgtcgaag gtctgcaggt cgatcgtcgt ccccagggcc gcggccgtca tcggcaactc
     1021 gacccggcag tgcaggtcgt ccccccgccg cgtgtaggtg gcgtggttcg ccacggcgac
     1081 ctcgacatac aggtcgcccg ccgagccgcc gccggtgccg acctcgcctt cgccggccag
     1141 ttggatgcgg gtgcccgagt cgaccccggc cggaaccttg agggtgaggg tgcgccgggt
     1201 gcggacccga ccctcgcccg agcactcgaa acacgggtgg ttgatcgtcg agccgtagcc
     1261 ctggcaggcc tggcagggcc gagtcgtcat cacctgcccg aggaaggacc gctgcaccgt
     1321 ctgcacctcg ccgcggccgt ggcacaggac gcaggtcgac acgccggtcc ccggctggca
     1381 accgtccccc tggcaggacg agcatcggac ggcggtctcg atggtgagct cctcctcaga
     1441 gccgaagacc gcgtcgtgca ggtcgatctg gatccggacg agggcgtcct ggccgcggtg
     1501 cgcccgcgag cgcgggccgc gctgggtggc cgcgcccccg aagaaggcgt ccatgacgtc
     1561 gctgaacgag aagccctgcc cgctgaagcc agcgccccca cccgcggcga aggggtcgac
     1621 accgaggtcg tagttgcgcc gcttgtccgg gtcagagagc acctcgtagg cccgagacac
     1681 cgtcttgaac tgttcctcgg cctcggcgcc cgagttgaca tcagggtgca actggcgggc
     1741 gagccgtcgg taggcacgct tgatctcctc cgccgtggcg gagcgttcga cgccgaggtc
     1801 ctggtagtag tcgctcacaa ttccttctcg gtgcagtggc tgtcggtgct cgatcagggc
     1861 tcaagcagct cggagacgta atgggctacg gcgcggacgg tcgccatggc gttggggtaa
     1921 tccatccggg tggggccgag gatgcccagg cccgcaacga gctcggcacc cgtgccgtag
     1981 gccgccgaga caaccgacgc cccggccagg ccctggtgtt cgtgctcatg accgatggtc
     2041 accgcgaccg cgccgccctg gccctggcgc agttggccga acagcttgag cagcacgacg
     2101 tgctcctcga gcgcttcgag caccggtgcg atcgtcagcg ggaagtcggc gccgaaccgg
     2161 gcgaggttgg acgtccccgc caggacgacc cgctcctccc gctcctccgc gagggcgtcg
     2221 tccagcgtgc gcagcacgat gcgaaccaga tcgcgatcgg ccggctcgaa acgctcgggg
     2281 agggattgca ggcgggccgc agcgtccggg aaggaccgtc cggcggcctc gccgagcacc
     2341 tcggagcgga tccgggacac gagggcggcg ccggtctcgt cgtccaggcg gcagtgggcg
     2401 tcgacgacgc gctgttcgat ccgcccggtg ttggcgatga ggatgaccat gagccgatca
     2461 cgggccatcg gcacgagctc gatgtgtcgc accgccgacc gggtgagcga cgggtattgg
     2521 acgacggcga cctggtgggt gagcgacgcg agcaggcgta cggtccggtc cacgacgtcg
     2581 tcaagatcga cggcaccctg caagaagtgg ctgatcgctg accgttcggc accggacatc
     2641 ggcttgaccg cactgagccg gtcgacgaac acccgatagc ccgcgtcggt cggcacccga
     2701 ccggccgacg tgtgcggcgc atggatgagc ccctcctcct cgaggatggc catgtcattg
     2761 cgcacggtcg ccgccgaaac gccgaagtgg tggcgttcga cgaggctctt ggaccccacc
     2821 ggctcggacg tgcggacata gtcctggacg atggctcgca acaccgacag ccgccgctcg
     2881 tcgctcatcg ccctccctcg tcccgctcgt cggtcctacg cccacccgtc tcggcagcgc
     2941 actggcactc tcaccacgtg agtgccaagt ctacgtgggt ctggcggcgc gtccacccgc
     3001 tcggcgcgcg gacgcggcat accctgccgg gatggatcgc tacggtcgcg acgtcctcgc
     3061 cgacgcagca cgtatgccgc gtcgccccgt cgccgctccc gttgccgcgg agccgggcct
     3121 ggtcgtcgag gacgtcgaga ccggctggtg cggcgcggtc gtgcgcgtgg agaaggccgg
     3181 tggcatgcat gtcgtccacc tcgaggaccg ccgcggccgg accaagggct tcccgctcgg
     3241 gccgggcttc ctccttgaag ggcgtcccgt ggtcctcacg ccgccggtgg cggccgaccg
     3301 cgccaagttg gaggcggtcc gcaaggctgc ggcgcgcacg gccagcggtt cggtggccgt
     3361 ggtcggccaa cccgcccggg tcgccgtcgg ctcccggatc tgggtcgagg gacgtcacga
     3421 tgccgagctc gtcgagaagg tctggggtgc cgacctgcgg gtcgaaggcg tcgtcgtcga
     3481 gcagttggac ggcatcgacg acctcgtggc cagcgtgcgg tccttcgccc cgggtccggg
     3541 acggcggctc gggatccttg tcgaccacct cgttccgggc accaaggagt cggccatcgc
     3601 ggcggcagtg cgggcgctcc ccgctgccac gcaagggcat gccctgctgc tcggccaccc
     3661 gtatgtcgac gtctggcagt cggtcaagcc ggcccggttg gggtggaccc aatggcccgt
     3721 catcccgcgc ggcaggtcct ggaaggaagg aatctgccag gcgctgggct ggccctgcga
     3781 cactcccgcc gaccttgcct ttgcctggaa acgcatcctt ggcacggtgg gctcgtatgc
     3841 tgatctggag ccaaccctgc tcgcggccgt cgaagagctc atcgacttcg tcaccgcacc
     3901 aggcgcctag tttcaggcgt ccgacaccgg aggtaccaat gtcagaccag cccgccaacg
     3961 acccgacccg ccccgtcgac gttccgcccc cgccgccgcc cgcctggagc ggctcggccc
     4021 ccgaggcacc cgccgcgccg gtcccggcac cccccgccta ccaggctccg cccgccgcct
     4081 accagacccc ggcagcgccg cccccgcccg cctacccgcc gcaggggtat gccgcccccg
     4141 tcgccggagt cgtgccgctc agccctgccg agtcacgcca gtgggggatg ctctcgcatg
     4201 tcggcggcat catcctcggc ttcgtcggcc cgctcatcgt catgctcgtg ttcggccccc
     4261 gggacgcctt caccaaggac cagtccaccg aggccctcaa cttccagctc accctgctga
     4321 tcggctacat cgccgggacg atcctcacgt tcatcctccc ccttcccgtc gggctcatcg
     4381 tctgggtcct cgggctggtt ttcgcgatca tgggcgcgtt ggccgccaac aagggcgagg
     4441 cctaccgcta cccgttctcc ctgcgcctgg tcaagtagtc gcaggccgct gacagtttcc
     4501 gcgtgcgccg gttggtggat cagcccacca gccggcgcac cactgtgtcg gccaacaatc
     4561 ggccggtcag ggtgaggcgg acccggcgct cggccagggc ggcccggccg tcgacgagtc
     4621 cctcggcgac cagcccagcc acttcacgcg cggccccggc ggcgaggtcg gcgatcggca
     4681 ggccatcgac cagccgcacg ccgagcagca cgcgttcgtc ggcctgctgc tcgctggtga
     4741 ggacctcgcg ggcggccgcc gggctgagga cgggtccgag tgcagcgcta ccacgcggcg
     4801 cgccgatgcc cgcgtcgccc aggcgggcgg catacgccct cgggtgtttg acgttccacc
     4861 accgcacgcc gccgacgtgg ctatgcgctc cgggaccggc g
//
LOCUS       DAOMZY010000275         4839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_16527_length_4839_cov_7.966764, whole genome shotgun sequence.
ACCESSION   DAOMZY010000275 DAOMZY010000000
VERSION     DAOMZY010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16527_length_4839_cov_7.966764"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            913..4212
                     /locus_tag="PLK64_13495"
     CDS             913..4212
                     /locus_tag="PLK64_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019159031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPV80804.1"
                     /translation="MPKIFDNLTPESQLGVALRDYFGGSYESLDVATGYLDLRGWGHL
                     GDIIGGKPFAEGDPPVARVLVGMVAPSDSQAILSSLQAELAEQGAGAAIHDNSKALDQ
                     KERLVRHLRTQLMRGLPTKSGQATLQLMKRQLESGRVQIKVFTRRPLHGKAYILRGGG
                     GPAPLWSYLGSSNFTGAGLFNNLELNIDIADQDPNQKLASWFEDRWDDKYSLTITKEI
                     IELIEESWSGEEQATPFEVYLKVCHALSQDARDGLGYVLPKSMQDLLLDYQVTAVRTL
                     ARRIVRRGGTMLGDVVGLGKTLTAVATALMLQAAEDYTTLVLCPKNLESMWQEHLDAY
                     GVEGARVVPYSMADKVLPDLKLYKLVICDESHNLRNDTTRAHEAISEYVRRNSSKVLL
                     LTATPYNLAFADVANQLSLYIEEDEDLGIVPSAAMAKDHTLADKVDGKTNTLVAFKRS
                     EESDDWRRLMSDHLVRRTRSFIKKSAKKKLITLTDGTVQEREYLQFADGTEFFFPTRV
                     PHPLTHTFGKDDPASLMEDDTTLDAIAKLTLPRYRLSDYEDTRATKTAEDKKYLDDIR
                     SGRGNVSGFVRIGLFKRLSSSGHSFILSLQRQRARNELFIHAIDHKLQVPLGSFTDRQ
                     IAVSDDDLESDEPVGGSLASRYQELRDSAPAKTKWLNSTVFKTTLRHDLIKDNELITS
                     LLVRFGSWDSTRDTKVNALVDLLRGDHEGQKVLIFSEYKDTAEYVADALEAAGVDNVG
                     LAAGDVADPSDVARRFSPRSNRLPGQSVSEVAEPADPIDVLVATDVLSEGQNLQDSHV
                     IVNYDLPWAIIRLIQRAGRVDRVGQESDTVHVYLITHDKVEQQIRLRQRIRARLSAAA
                     EAFGSDEQFFGTDDEINLLHNFYNGNLSDGDDDGEGEADAVSEAWLAWSNAQNSHPEV
                     TKRVLALPDLIHSTRDKYARESTGGVACFVRTESGVDAFAVSESVAGAERLLTPAEAL
                     RIFEAEPTTPTASPRDDHFEREAELVRGPLTIEARAAGNLRGVRKRIWERFGGTFYAE
                     QAEDALNALHERPLTTHATNQLSIALRNKYPDQDILDLLDRLHREDRLVIGSSESDEL
                     RIVCSIGVTDQ"
     gene            4209..>4839
                     /locus_tag="PLK64_13500"
     CDS             4209..>4839
                     /locus_tag="PLK64_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HPV80805.1"
                     /translation="MSQDLLPKVDRGAFQEVFIEDLNWLAPDVKQSISVTDDDGRQVS
                     AHNVASYKGLRVWVCDERPGSVLEAELDRLIAKTTTDRLVIFDDEKEQVWRWPVRRAK
                     DGSVTSRLTSHRHKKGETDPKFAARLDIIRMPFDVILDANAVLGRVREAFDTEAQNES
                     KRASKLMANMYAAVEKAYPATVAPKVRDHEISVTLARILFLMFGDDTEMW"
BASE COUNT          961 a         1624 c         1468 g          786 t
ORIGIN      
        1 aacaaccact cggtccccaa ctcgggagcc agcaccacag gggctggccg cttccgggca
       61 ggcgaaatag actcgtgcac gcgacggcac cttcgcttct gggcaacagt tgcatcggga
      121 ccgtcgccga ggggtacccg ttggcgcggg tgcccctcat ccgtgtgcgg atgaggtctc
      181 ggatcttgtc cgagtccgag cctacggcga ggctcggcct gaatcaaggg ttcgttgctg
      241 aataagcgac acacacaggt gcgcaagcct ggctgtatat cttggcgatg tcggttcacc
      301 acggcgcccg gaacagcggg ccggacaggc gagaacacga tgtcgcaggt cgcgccgtct
      361 ctgtcgctta ttcagcatgc gaatgagact ttgacctacc ctttgatcgt ggccgagtcc
      421 ggtagggtcg aatcgacggg tctgaccaac ctgtcactga acacgccggg tgccaccaac
      481 gctcggcgcc agggtctgac caaccccagc gaaggacgtc taccggctgt gccccagcga
      541 atcaacactg ccgttgcccc ggacgaagcc accgtcccca aagtggccag cgaatggcca
      601 accgccgtcc agccccagga gtcattcctg tgcatgaatc cgcgatgccc cgaagcggtg
      661 cggatcaccc ggaacaacac tcgtggacgc ccacagctgt tctgcggcac aaaatgtcgg
      721 cgcgcatacg actacgagcg cgctcagctc cggctcgatc atgaacggct caaggcagcc
      781 gtggcccgcc cagggggcac cttccgtgag cgtcaagttg tcgaggccgc cctcgccagc
      841 gtcgagaggt gcctacttca ctacacgtac cctggcccca ctgcgacaca aggctgaggc
      901 aggatcacca ctgtgccgaa gatctttgac aacctcacgc cggagagtca gctgggcgta
      961 gcccttcggg actactttgg cgggtcctac gagtcgttgg acgtcgcgac tggctatctc
     1021 gacctgcgcg gctggggcca cctcggagac atcatcggcg gcaagccctt cgccgagggg
     1081 gacccgcctg tcgctcgggt cctcgtgggg atggttgccc cgtccgactc ccaggccatc
     1141 ctgagctcgc tccaagcaga gcttgcggag caaggggccg gcgcggccat ccacgacaac
     1201 agcaaggcgc tcgatcagaa agagcggctc gtgcgccacc tgcgaacgca gttgatgcgc
     1261 gggctgccga ccaagagcgg gcaggccacc cttcagctca tgaagcggca gttggagtcg
     1321 gggcgtgtcc agatcaaggt gttcaccagg cgaccgcttc acggcaaggc gtacatcctt
     1381 cgcgggggcg gtggccctgc cccgctgtgg tcctacctcg ggtcctccaa cttcacgggc
     1441 gccggcctgt tcaacaacct tgagctcaac atcgacatcg cggaccagga ccccaaccag
     1501 aagctggcgt cgtggttcga ggaccgctgg gacgacaagt actccctgac gatcactaag
     1561 gagatcatcg agctgatcga ggagtcgtgg tccggtgagg agcaggccac accattcgag
     1621 gtttacctca aggtttgtca cgccttgtca caagatgctc gcgacggcct ggggtacgtg
     1681 cttcccaagt cgatgcagga ccttctgctc gactaccagg tcacggctgt gcgaaccctg
     1741 gctcgtcgga tcgttcgccg cggcggcacc atgttgggcg acgtcgtcgg gttgggcaag
     1801 acgctcacgg ccgtggccac tgccctgatg cttcaagccg ccgaggacta caccaccttg
     1861 gtgctgtgcc cgaagaactt ggagtcgatg tggcaggagc acttggacgc ctacggggtg
     1921 gagggcgcgc gcgtcgttcc ctactcgatg gctgacaagg tccttccaga cctgaagctg
     1981 tacaaactcg tcatctgcga tgagtcacac aaccttcgca atgacaccac cagggcccac
     2041 gaggcgatca gtgagtacgt ccggcgcaac tccagcaagg tcctgctcct caccgcgact
     2101 ccctacaacc tggccttcgc cgacgtcgcc aaccaactgt ccctgtacat cgaagaggac
     2161 gaggacctgg ggattgtccc ctcggcggcg atggccaagg accacaccct ggccgacaag
     2221 gtcgacggca agacgaacac gctcgtggcg ttcaagcgct ccgaagaatc tgacgactgg
     2281 cgccggctca tgtccgacca cctcgtgcgt cgcacccgca gcttcatcaa gaagtccgcc
     2341 aagaagaagc tgatcaccct cacggacggg acagtccaag agcgcgagta cctccagttc
     2401 gccgacggca ccgagttctt cttcccgacc cgggtcccgc atccactcac gcacacgttc
     2461 gggaaggacg acccggcatc gttgatggag gacgacacca ctctcgacgc gatcgcgaaa
     2521 ctgacactgc cgcggtatcg gctctccgac tacgaggaca cccgagcaac caagactgcc
     2581 gaggacaaga agtacctgga cgacatccgc tcaggccgag gcaacgtcag cggcttcgtg
     2641 cgcatcggcc tgttcaagcg cctctcgtcg tccgggcatt cgttcatcct gtccctgcag
     2701 cgtcagcgag cccggaacga gctcttcatc cacgcgatcg accataagct gcaggtcccg
     2761 ctggggagct tcaccgatag gcagatcgcc gtcagcgacg acgacctcga gagcgacgag
     2821 ccggtaggtg ggtcgctggc cagtcgttac caggagctgc gcgacagcgc accggcgaag
     2881 accaagtggc tcaactccac ggtcttcaag accacgctgc ggcacgacct catcaaggac
     2941 aacgagctga tcacgtccct cctggtccgc ttcggcagct gggactccac gcgtgacacg
     3001 aaagtgaacg cgctggtcga cctgctacgc ggtgaccacg agggccagaa ggtgctcatc
     3061 ttcagcgagt acaaggacac cgccgagtac gtcgcggacg cgcttgaggc cgcgggtgtc
     3121 gacaacgtcg ggttggcggc gggagacgtg gctgacccct ccgatgtcgc ccgccgattc
     3181 tccccacgct ccaatcggct cccaggccaa agcgtcagcg aggtggccga gcccgctgac
     3241 cccatcgacg tcctcgttgc cacggacgtc ctctcagaag gccagaacct tcaagacagt
     3301 cacgtgatcg tcaactacga cctgccgtgg gcgatcatcc ggctcatcca gcgcgccggt
     3361 cgagtggacc gtgtcggaca ggagtccgac acggtccacg tctacctgat cacccacgac
     3421 aaggtcgagc agcagattcg gctgcgccag cgcatccgtg cgcgtctcag tgcggccgcc
     3481 gaagcattcg gctccgatga gcagttcttc ggaaccgacg acgagatcaa cctcctccac
     3541 aacttctaca acggcaacct ctctgacggc gatgacgacg gagagggcga agcagatgcc
     3601 gtgagcgagg cctggctggc gtggagcaac gcccagaaca gccaccccga ggtgacgaag
     3661 agggtgctcg cgctcccgga cctcatccat tccacgcgcg acaagtacgc ccgtgagtcc
     3721 actggtggcg tggcctgctt cgtgcgcacc gagtcagggg tcgacgcatt cgcggtcagc
     3781 gagtccgttg cgggcgctga gcggctgctc acaccagccg aggccctgcg catcttcgaa
     3841 gccgagccaa cgacccccac tgcatcaccc cgtgacgacc acttcgagcg cgaggccgag
     3901 ctggttcgag gtccactgac catcgaggcg cgtgccgccg gaaacctccg cggtgtccgc
     3961 aaaaggatct gggagcgatt cggcgggacc ttctacgccg aacaggccga ggatgcactc
     4021 aacgccctgc acgaacggcc actgacaacc catgccacca accagttgtc gatcgcgctg
     4081 cgaaacaagt accccgacca ggacatcctt gacctgctcg accgacttca ccgcgaagac
     4141 cgtctcgtca tcggctccag cgagtcagac gagctacgaa tcgtctgctc gatcggagtg
     4201 actgaccagt gagccaggac ctgctgccga aggtggaccg cggcgccttc caagaggtct
     4261 tcatcgagga cctcaactgg ctggcgcccg acgtgaagca gtcgatcagc gtgactgacg
     4321 acgatggccg ccaggtcagc gcccacaacg ttgcgtccta caagggcctt cgcgtgtggg
     4381 tctgcgatga gcgcccggga tctgtgctgg aggcggaact cgaccgcctg atcgcgaaga
     4441 ccaccaccga ccgactcgtc atcttcgacg acgagaagga gcaggtctgg cgctggccag
     4501 tgcggcgggc caaggatggc tcggtcacgt cacggcttac cagccaccgg cacaagaagg
     4561 gggagaccga ccccaagttc gccgctcgcc ttgacatcat ccggatgccg ttcgacgtca
     4621 tcctcgacgc caacgctgtg ctgggacggg ttcgggaggc cttcgacacc gaagctcaga
     4681 atgagtccaa acgggcatcc aagctcatgg ccaacatgta cgccgccgtg gagaaggcct
     4741 acccggcaac ggtggccccc aaggtccggg accacgagat ctcggtgacc cttgcccgga
     4801 tcctcttcct catgttcggc gacgacaccg agatgtggc
//
LOCUS       DAOMZY010000276         4835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_16541_length_4835_cov_5.642469, whole genome shotgun sequence.
ACCESSION   DAOMZY010000276 DAOMZY010000000
VERSION     DAOMZY010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16541_length_4835_cov_5.642469"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..807
                     /locus_tag="PLK64_13505"
                     /pseudo
     CDS             136..807
                     /locus_tag="PLK64_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777272.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1003..4041
                     /locus_tag="PLK64_13510"
     CDS             1003..4041
                     /locus_tag="PLK64_13510"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPV80806.1"
                     /translation="MATITTPPLATAPAAPSRDGVGEWRAGWRVALRLARRDVRRHVG
                     RSLLIVLMVAVPVLLLVGGNIVFSSQDLTAAERIPYAMGQTQAVATYSGGIQIPPQVE
                     PWFGYYGQGMPKSDLATPVPGWGTDLATHAQALGALAGATAIPITEDGGYTTIGRRQI
                     DLQVRGVDAARYSTQTQGIVRLESGRWPTSSTEAVVTKVGVFHGLPSSGTLAVTDRSG
                     TLTTYTIVGVGEGWIAAYSLQPVDLITLPAAGALENGVAAYLIDRPTPVTWDEVKRLA
                     TYGLQVTSRQVLLNPPSREELALPDGVQSMADNAAAGQAITSAIMAIGLLLETTLLVG
                     PAFAVSAARQRRTLALAASNGATTAQLRRTVLAQALVLGVLAALVGAVVGVLGAWAVI
                     RWSGIYRPDTMFGPFDVPVLPVAIIVGCAVASAVIAAMIPSRGLGRLDIVAVMRGQSV
                     SPPALVRTPIAGIVLAGLGAVGVFWAVAFTSGAERTWIDDVVPLVAMGGAIMLVIGAL
                     LLVPMVLVLAGRVARSAPVAIRMALRDAARQRGRATSTVAAILGGTALLSAILVVAAS
                     DTAYRAKTYVPQLPMGQALLSPTTAMDGSLADPRWTQNVTDIAHGIDPALQVHEIALV
                     DVSRTGGAIQKPIGPESPMVMPFFVAVRTGCALAQALDMGAPIGADGPPDMSCLSLNG
                     RGMSEGRSAILVGTVAQLVQTYTLDATAEATLRAGGLVVAGDKAVTPTWTKMPEGGWG
                     SSGPILGQVDIVDGRVSLARGTATYGSEAGPTLSNVTTLSLAATPVPAATLNATDAGV
                     PGPAFHGMTMVGALMTVETAKSLSLPTQTIETRILDPRGPLSAEAGAALSQSFADSNL
                     GWFYVERGFQPYDTVLAAIVLSFIGLIILVATLVSTALSTAETQSMMGTFAAVGATRS
                     TRRNLAAAQAGSLGVIGALLGTLVGFVPGIALARSFTRYPTESVAYGEYTQGAPALLD
                     PTVVIPWLQLAIPVIAVPALAALLAWLAIRKAPTVTRRLT"
     gene            4192..4809
                     /locus_tag="PLK64_13515"
     CDS             4192..4809
                     /locus_tag="PLK64_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial proteasome activator family protein"
                     /protein_id="HPV80807.1"
                     /translation="MSDQPDTSGSGADENRIVVITPDGMGVATSGHGDDEGHDSRDSR
                     EVTNPADLVEQPAKVMRIGSMIKNLLDEVRNAPLDEAGRLRLAEIHRRSIDELKDGLA
                     PELVEELDRIALPFGAGAPSDAELRIAQAQLVGWLEGLFHGIQTALVAQQMAAQQQLA
                     GMRRALPPGHPDAGPAGPGMPPGFPGGPGQGQGEQGHPGPTGQYL"
BASE COUNT          679 a         1788 c         1633 g          735 t
ORIGIN      
        1 ccggtcgcgc gtcggcgacg agcttcccta ccatgtcacg ggatggtaca accagatcgc
       61 ccgcgcccag gcggaggccc gcatctccgc gacaccctcc cacccagctc acaccgtccc
      121 aacaggggga accccatgag cgacctcgtc ctgcgtatga ccgacgtcta ccgcgtccac
      181 ggctccggcc cgaccgcggt ccgcgccctc gacggcgtga gcctctccgt ccaggcgggc
      241 gagctcgtcg ccgtcatggg tccgagcggc agcggcaagt cgacgctgct caacctcgcc
      301 ggcgccctcg acctgcccac cgcgggcgtt gtcgagatcg acgggcagtc gacctcctcg
      361 ctgcgtcccg ccgacctcgc cgccctgcgc cggcgtcggg tcggtgtggt cttccaagac
      421 ctcaacctca tcccgtcact caccgccgcc gaaaacatcg gcctgcccct tgaactcgac
      481 ggccgtcggg tgagcgaggc ccatcgcgcc gccgtcggcg ccctgcacga gttgggcttg
      541 gacgagctcg ccgaccgcta ccccgaccag atgtccggag ggcagcagca gcgcgtcgcg
      601 atcgcccgcg cgctcgtcgg cgaccgccgc ctggtcctcg ccgacgaacc caccggcgcg
      661 ctcgactcgc tcaccggcga gggcgtgctc gccgtgctgc gggcccgttg cgacgcgggc
      721 gcggccgggc tgctggtcac ccacgaggcg cggcacgccg cctgggccga ccgggtcgtc
      781 tacctgcgcg acggtcgggt cgtcgactcc accgaggcct acagcgccgc gagtatgccg
      841 cgcgcgcggc atacccctgc gccgtcgggc acgtcgcctt cccctgggtc aagcccggtc
      901 gcgggtatgc cgcccgcccc cggccccagc ggggccgtca cgcctcccgg ggccaccgcg
      961 tcggtcggcg cccacagcga gcccgggcgc gagggctgag cgatggcgac catcaccact
     1021 cctcccctgg ccactgcccc tgcagccccg agccgcgacg gcgtcggcga gtggcgggcc
     1081 ggctggcgag tcgctctgcg cctggcccgg cgcgacgtgc gtcgccacgt cgggcgcagc
     1141 ctgctcatcg ttctgatggt tgctgtgccg gtgctgctgc tggtcggcgg caacatcgtc
     1201 ttctccagcc aggacctgac cgcggccgag cggatcccct acgcgatggg ccagacccag
     1261 gcggttgcca cctactccgg cggtatccag atcccgcccc aggtcgaacc ctggttcggc
     1321 tactacggcc aggggatgcc caagtccgac ctagccaccc cggtcccggg atggggcacg
     1381 gacctggcca cccacgccca ggcactcggc gctttggcag gcgccacggc gatcccgatc
     1441 accgaagatg gcggctacac gacgattggg cgacggcaga tcgatctgca agtgcgcggc
     1501 gttgacgccg cgcggtactc gactcaaacc cagggcatcg tccggctcga gtcgggccgc
     1561 tggccgactt ccagcacgga ggccgtcgtc accaaggtcg gggtcttcca cgggctgccc
     1621 tccagcggaa cgcttgcggt tacggaccgg tccggcactc tcacgaccta caccatcgtc
     1681 ggtgtcggcg agggatggat cgccgcctac tccctgcagc cggtcgacct catcaccctc
     1741 ccggccgcag gtgccctgga gaacggcgtc gcggcatacc tcatcgaccg tcccaccccc
     1801 gtgacgtggg acgaggtgaa gcggctggcg acctacggac tgcaggtgac cagccgccag
     1861 gtcctcctca acccgccgtc ccgcgaggag ttggcgctcc cggacggcgt ccagtcgatg
     1921 gccgataacg cagcggcggg gcaggcgatc acctcggcga tcatggccat cgggctgctg
     1981 ctggagacga ccctcctcgt tgggccagcc ttcgccgtca gcgcggcccg gcagcgacgg
     2041 accctcgccc tcgcggcgag caacggggcg acaaccgccc agttgcgccg gaccgtgctg
     2101 gcccaggcgc tggtgctcgg tgtcttggcc gccctcgtcg gtgccgttgt cggtgtgctc
     2161 ggcgcgtggg cggtcatcag gtggtctggg atctatcgac cggacacgat gttcggcccg
     2221 ttcgacgtgc cggtcctgcc ggtcgcgatc atcgtcgggt gcgccgtggc gtccgccgtc
     2281 atcgccgcga tgatcccctc gcgcgggctg ggtcggctgg acatcgtcgc ggtcatgcgt
     2341 ggccagagcg tgtcgccacc ggcgctcgtg cgcacgccca ttgcgggcat tgtgctcgcc
     2401 ggactggggg ccgttggggt cttctgggca gtggcgttca cctccggcgc cgagcggacc
     2461 tggatcgacg atgtcgtgcc gctggtcgcg atgggtggag cgatcatgct cgtcattggc
     2521 gccctcctgc tggtgccgat ggtgctcgtg ctcgccggcc gcgttgcccg gtccgcgccc
     2581 gtggccatcc ggatggcgtt gcgcgacgcc gcgcggcagc ggggacgggc tacctcgacg
     2641 gtggcggcga tcctcggcgg gaccgccctg ttgtcggcga tcctcgtcgt ggctgccagc
     2701 gacacggcct atcgagcgaa gacctacgtg ccccagttgc ccatgggtca ggccctgctc
     2761 agccccacga ccgcgatgga cggcagcctg gcggacccac ggtggaccca gaacgtcact
     2821 gacatcgcac acgggatcga cccagccctg caggtccacg aaatcgcgct cgttgacgtg
     2881 agccgcaccg gcggcgccat ccagaagccg attgggccag agtccccgat ggtcatgccg
     2941 ttcttcgtcg cggtgcggac cggatgcgcc ctcgcacagg cgttggatat gggggccccg
     3001 atcggcgccg acggcccacc cgacatgagt tgcctctcgc tcaacggcag gggtatgagc
     3061 gaaggacggt ccgccatcct cgtgggaacg gttgctcaac tcgttcagac ctacacgttg
     3121 gacgccacgg ccgaggcgac cctgcgagcc ggcggccttg tcgtcgcggg cgacaaggcg
     3181 gtgaccccga cctggacgaa gatgcctgag ggcgggtggg gctcctccgg cccgatcctg
     3241 ggccaggtgg acatcgtcga tgggcgggtc tccctcgccc gaggcacggc gacctacggc
     3301 tccgaggcag ggccgacgtt gagcaacgtg acgaccttgt cgttggctgc caccccagtg
     3361 ccggccgcga ccctcaacgc gaccgacgcc ggagtccctg gcccggcctt ccatggaatg
     3421 accatggtgg gcgcgctcat gaccgtcgag accgcgaagt ccctgtcgct gccgacgcag
     3481 accatcgaga cgaggatcct cgacccgcgt ggcccgctca gcgccgaggc tggggcagcc
     3541 ctctcgcaga gcttcgccga cagcaacctc ggctggttct atgtcgagcg gggcttccag
     3601 ccctacgaca cggtgttggc ggccatcgtc ctgtcgttca tcggcctgat catcctcgtg
     3661 gccaccctcg tctcgacggc gctgtcgacg gccgaaactc agtcgatgat ggggactttc
     3721 gcggcggtcg gagccacgcg atcgacccga cgcaatctcg ctgcagcgca ggcaggttcg
     3781 ctaggtgtca tcggtgcact gctggggacg ctcgtgggct tcgtaccggg gatcgccctg
     3841 gctcggtcct tcacgcgcta tccgacggag tccgtggcat acggcgaata cacacaggga
     3901 gcacccgccc tcctggatcc gacggtcgtc attccgtggt tgcagttggc catcccagtc
     3961 atcgcggtcc ctgccctggc cgcgctgttg gcctggttgg cgatccgcaa ggccccgacg
     4021 gtgacccgcc gcctgacctg agcgcttctg cacgcaaccg tttggccgac tgcgacgtct
     4081 caaccgacac aggggatcga cctccgcgga cgcgtgggcg gcatacacag ggggtatgcc
     4141 gcccaccgag tcccggagcc cccgccgggc cgagtgcggc aagatggagc catgagcgac
     4201 cagcccgaca cctctggatc cggcgccgac gagaaccgga tcgtcgtcat cacgcccgat
     4261 gggatgggcg ttgccacctc gggacacggc gacgatgagg gtcacgactc acgcgactca
     4321 cgcgaggtga ccaacccggc cgacctggtc gagcagccgg ccaaggtcat gcggattggc
     4381 tcgatgatca agaacctcct cgacgaggtg cgcaacgccc cgttggacga ggcgggtcgg
     4441 ctgcggctgg ccgagattca ccgccgctcg atcgacgaac tcaaggacgg tttggcgccg
     4501 gagttggtcg aggaactcga ccggatcgcg ctgccgttcg gcgcgggagc accaagcgac
     4561 gccgagctgc ggatcgcgca ggcccagctc gtcggctggc tcgagggcct tttccacggg
     4621 atccagaccg cgttggtcgc ccagcagatg gccgcccaac agcagttggc cgggatgcgc
     4681 cgcgcgctgc cgccgggaca ccccgacgct ggcccagcag gccccggtat gccgcccggc
     4741 ttccccggcg gccccggcca gggtcaaggc gaacaaggcc accccggccc gaccggccaa
     4801 tacctctagc cctcgccccc accttcggtc ttggc
//
LOCUS       DAOMZY010000277         4816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_16638_length_4816_cov_5.068263, whole genome shotgun sequence.
ACCESSION   DAOMZY010000277 DAOMZY010000000
VERSION     DAOMZY010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16638_length_4816_cov_5.068263"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PLK64_13520"
     CDS             <1..506
                     /locus_tag="PLK64_13520"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPV80808.1"
                     /translation="AFPPGSVIGLWRFGYHLGGPTQDWQEMVPQRGLNDVVGGKDQRA
                     LLAEGFATLAARPGGSTGLYDTILAAYEQAKAGYDPTRINSIVLLTDGKNEDPRSITL
                     DDLLAKLAAAKDPARPIVVITVAIGPDADAATLARVTEVTGGRSYTATAPEQITGVFV
                     DALLNRS"
     gene            618..1424
                     /locus_tag="PLK64_13525"
     CDS             618..1424
                     /locus_tag="PLK64_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HPV80809.1"
                     /translation="MRAYLRTYWLLARAGFRRQATYRMALAAGIFTNSVFGVVRASIL
                     VGAVTGAGAAVAGYDALAVSSYAWWGQVLLGSVSMWGWSEVADRVRTGDIAADFLRPV
                     PPYLTYLAVDLGRAALALLARGAPMLLIGALYSGITFATGAVEWMLGLTAVALAVAVG
                     FLGNYLVNCAAFWLVEIRGLLMAYSVVATLLAGLYVPVHWFPEWLRTVAAATPFPSLI
                     QTPIDILSGYAAGPAAVRAVAVQVGWVLALGVAGALATAAGRRRLEVQGG"
     gene            1417..2220
                     /locus_tag="PLK64_13530"
     CDS             1417..2220
                     /locus_tag="PLK64_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HPV80810.1"
                     /translation="MASGFTAYRAVLRSRVRAQQGYRVSFATDLFATVLTGLAELLEV
                     YVIFHNTPRLGGLDFDAALLVFALANLTFAAADLLVGHCDRLPTYVREGRIDVFYLRP
                     LSILGQLMTSEVSLRRLGRISVAVVVLGIALVRGEVAPTPTTVGMLALSIVCGTALMA
                     GLFVAVAGLQFWLVDGREFANAFTYGGAYAAQQCSLVFPDPVRIFFTFVVPAAFTAYL
                     PTLVILGEPGGDFADPALAWWLPAATAWVWGVALLTWRHGVRHYQGAGG"
     gene            2223..3212
                     /locus_tag="PLK64_13535"
     CDS             2223..3212
                     /locus_tag="PLK64_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPV80811.1"
                     /translation="MSVEPVRPVSASGPVSAPIVAVEGLTRVFRRRGAPALTAVDAMT
                     FRIAPGEAVGYIGANGAGKSTTIKMLTGILAPTSGTARTVGLDPMRDRRELARRVGVV
                     FGQRSQLWWDLPLRESFDLLAAIHRLDRLRATRRTGQLVELLDLGPTLAQPVRSLSLG
                     QRMRAEVAAALLHEPELVILDEPTIGLDVLSKARLREFLIQTRREHGTTLLLTTHDMG
                     DIERLCDRVLVVDHGRLAFDGTLAGLAGTVGARRVLVVDLDRPSEPLHGIPHTQWLGA
                     EGEGVRQRLAFDPADTTAAAVLAAVSERVTLRDLSIEEPDIEDVVRRIYSASS"
     gene            complement(3238..3738)
                     /locus_tag="PLK64_13540"
     CDS             complement(3238..3738)
                     /locus_tag="PLK64_13540"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018780104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HPV80812.1"
                     /translation="MTCLSTRPARTSDVRAIREIVAPLVAKRRLVAKDAVAYYEGIQQ
                     FRVVQDGDTVIGCGALHVMWEDVAEVRTLAVAAAYLGRGAGHLLLEALIEDARTLGVQ
                     RVFCLTFETTFFADHGFQVIEGQAVPPDVYAEVVRSYDEGVAEFLDLERVKPNTLGNT
                     RMLLTL"
     gene            complement(3764..4183)
                     /locus_tag="PLK64_13545"
     CDS             complement(3764..4183)
                     /locus_tag="PLK64_13545"
                     /inference="COORDINATES: protein motif:HMM:NF039679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6188 family protein"
                     /protein_id="HPV80813.1"
                     /translation="MADVGLEPLLREEGAHYVLPLVGREVDQLCIDYAVTLVVDGTDT
                     IRLESPFTLHVDGTTQVVDPERLETVAAVLQLFRAVVTRAEAGKDGSLTIDFDSDRWL
                     RAEAIEDGEAWEIGGGLPPVTPSYFIAAQSGGGVRLS"
     gene            complement(4231..>4816)
                     /locus_tag="PLK64_13550"
     CDS             complement(4231..>4816)
                     /locus_tag="PLK64_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPV80814.1"
                     /translation="DFKNTVIIMTTNLGTRDITKGSLGFSAGPDTRSEYERMKNKVTD
                     ELKNHFRPEFLNRVDDVIVFPQLTQDEIVAIVDLEIAKLDKRLRDKDMGLELTMAAKA
                     LLAKKGYDPVLGARPLRRTIQREIEDMLSERILYGELRSGEFVLVDATGDEKDSTFTF
                     TGSLRKDTIPDIPDVPPIDEVGGPQDAQASASGT"
BASE COUNT          651 a         1684 c         1646 g          835 t
ORIGIN      
        1 cggctttccc gcccgggtcg gtcatcggcc tgtggcggtt cggctaccac ctgggcggcc
       61 ccacccagga ctggcaggag atggtgccgc agcggggcct caacgacgtc gtcgggggca
      121 aggaccagcg cgccctgctc gccgagggct tcgcgaccct cgctgcgcga ccaggtggga
      181 gcaccgggct ctacgacacg atcttggcgg cctacgagca ggccaaggct ggctacgacc
      241 ccacccggat caactcgatc gtgctgttga ccgacggcaa gaacgaggac ccccgatcga
      301 tcaccctgga cgacctgctg gccaagctgg ctgccgccaa ggaccccgcc agacccatcg
      361 tcgtcatcac cgtcgccatt ggtccggacg ccgatgcggc caccttggcg cgcgtcaccg
      421 aggtcacggg cggtcggtcc tacaccgcaa cggcgcccga gcagatcacc ggtgtcttcg
      481 tcgacgccct cctcaaccgg tcctgatcgc cgcgacgcag atccggcggg tcgggccggt
      541 ccctgtggcc ggtccgggcg gtcgccgcgg ccggtccggt tgctgcgcgg cataccccgg
      601 cgtagggtcg cgagtttgtg cgcgcctacc tccggaccta ctggctgttg gccagagccg
      661 ggttccgtcg gcaggcgacc tatcggatgg cgctggccgc ggggatcttc accaactcgg
      721 tcttcggggt cgtccgagcc tcgatcctcg tcggcgcggt gacgggcgcg ggcgctgccg
      781 tggccggtta cgacgccctg gccgtctcgt cctacgcctg gtggggtcag gtgcttctcg
      841 gctcagtgtc catgtggggg tggagcgagg tcgccgaccg ggtccggacg ggtgacatcg
      901 ccgccgactt cctgcggccc gtgcccccct acctcaccta cctcgccgtc gacctcggcc
      961 gagccgccct ggcgttgctc gcccgtgggg cgccgatgct cctgatcggc gccctctact
     1021 ccgggatcac cttcgccacc ggcgccgtcg agtggatgct ggggctgacc gccgttgcgc
     1081 tggccgtcgc cgtcggcttc ctcggcaact atctcgtcaa ctgtgccgcc ttctggctgg
     1141 tggagattcg cggcctgctg atggcctact cggtcgtggc cacgctcctg gccggcctct
     1201 acgtgccggt ccactggttc cccgagtggt tgcgcaccgt cgcggccgcc acccccttcc
     1261 cgtcgctcat ccagaccccg atcgacatcc tcagtgggta tgccgcgggg cccgcggccg
     1321 tccgcgccgt cgccgtgcag gtcggttggg tgctcgcgct gggcgtcgcc ggtgcgctcg
     1381 ccactgcggc cggacgacgc cggttggagg tccagggtgg ctagcgggtt cacggcttac
     1441 cgagcggtcc tgcgttcgcg ggtccgcgct cagcagggct accgcgtgtc gttcgcgacc
     1501 gacctcttcg ccaccgtgct caccggcttg gccgagctcc tcgaggtcta cgtcatcttc
     1561 cacaacactc cgcgcctcgg tggcctggac ttcgatgcgg ccctgctcgt cttcgcgctg
     1621 gccaacctca ccttcgctgc cgccgacctc ctggtggggc attgcgaccg actgcccacc
     1681 tacgtgcggg aaggccggat cgacgtcttc tacctgcggc ccctgtcgat cctcggtcag
     1741 ttgatgacga gcgaggtgtc gctgcgtcgg ctcgggcgga tctccgtggc cgtcgtcgtg
     1801 ctcggcatcg cactggtccg gggcgaggtc gcgccgaccc ccacgacggt cgggatgctc
     1861 gccctgtcga tcgtctgcgg aacggcgttg atggccggcc tcttcgtcgc cgtggccggg
     1921 ttgcagttct ggctcgtcga cggacgcgag ttcgccaacg cgttcaccta cggcggcgcg
     1981 tatgccgcgc agcagtgttc gctggtcttc cccgatccgg tgcggatctt cttcacgttc
     2041 gtcgtgcccg cagcgttcac ggcatacctg ccgaccctgg tgatcctcgg ggagccgggc
     2101 ggcgacttcg ccgatccggc gctggcgtgg tggctgcccg ccgcgaccgc gtgggtctgg
     2161 ggggtcgcgc tgctgacctg gcggcacggc gtgcggcact atcaaggcgc aggaggatga
     2221 gcatgagcgt cgaacccgtt cggccagtgt cggcatcagg gccggtgtcg gcaccgatcg
     2281 tggccgtcga gggcctcacg agggtgttcc gccgtcgcgg cgctcccgcg ctcaccgccg
     2341 tcgacgcgat gaccttccgg atcgcccctg gcgaggcggt cggctatatc ggagccaatg
     2401 gggctggcaa gtccacgacc atcaagatgc tcaccgggat cctcgcgccc acctcaggca
     2461 cggcgcgcac cgtcggcctg gacccgatgc gggatcgacg cgagctggcc cggcgggtcg
     2521 gggtcgtgtt cgggcagcgc tctcagttgt ggtgggacct ccctctgcgg gagtcattcg
     2581 acctgctcgc cgcgatccac cggctcgacc ggttgcgcgc cacccggcgg acgggccaac
     2641 tcgtcgagtt gctcgacctc gggccgacgc tggcgcaacc ggtgcggtcg ctgtcgctgg
     2701 gccaacggat gcgcgccgag gttgccgctg cgctgctgca cgagccggag ttggtcatcc
     2761 tcgacgagcc gaccatcggc ctggacgtgc tgtccaaggc ccgactgcgg gagttcctca
     2821 tccagacccg ccgcgagcac ggcacgaccc tgctcctgac gacccacgac atgggagaca
     2881 tcgagcgcct ctgcgaccgg gtgctcgtcg tcgaccatgg gcggctggcc ttcgatggga
     2941 cgttggctgg cctggccggc accgtcgggg ccaggagggt gctcgtcgtc gacctggacc
     3001 ggcccagtga gccgctgcac ggcataccgc acacgcagtg gcttggcgcc gaaggtgagg
     3061 gcgtgcgaca gcggttggcc ttcgacccgg ccgacacgac cgcggcggcc gtcctcgccg
     3121 cggtctccga gcgggtgacg ctacgcgacc tgagcatcga ggagccggac atcgaggacg
     3181 tcgtccggcg gatctactcc gcgtccagtt gaccgtcggg gcgcctggac gtgccggtca
     3241 gagggtcagc agcatccggg tgttgccgag ggtgttgggc ttgacccgct cgagatcgag
     3301 gaactccgcc acgccctcgt cgtaggaccg gaccacctcg gcatacacat ccggcgggac
     3361 ggcctgcccc tcgatcacct ggaagccgtg gtcggcgaag aacgtcgtct cgaaggtgag
     3421 gcagaagacc cgctgcacgc ccaaggtccg cgcgtcctca atgagcgcct ccagcagcag
     3481 gtggccggct ccgcggccca ggtatgccgc cgctaccgcg agcgtgcgga cctcggcgac
     3541 gtcctcccac atgacgtgca gggcgccgca gccgatcacc gtgtcgccgt cctgcacgac
     3601 gcggaactgc tggattccct cgtagtaggc gaccgcgtcc ttggccacga gtcgtcgctt
     3661 cgccacgagg ggggcaacga tctcgcgaat ggcccgcacg tcgctcgtgc gagccggtcg
     3721 ggtactcagg caggtcacga cagacgatcg taaggggtcc ggctcaggac aggcggacgc
     3781 ctccgccgga ctgggccgcg atgaagtagc tcggggtgac cggcggaagg ccgccgccga
     3841 tctcccaggc ctcgccgtcc tcgatggcct cggcacgcaa ccagcggtcg ctgtcgaagt
     3901 cgatggtcaa cgacccgtcc ttgcccgcct cggcgcgcgt gacgacagcc cggaacaact
     3961 gcagcaccgc ggcgaccgtt tccagtcgct cggggtcgac gacctgggtg gtcccgtcca
     4021 cgtgcaacgt gaacgggctc tccaggcgga tggtgtcggt gccgtcgacg acaagcgtca
     4081 cggcgtagtc gatacagagc tggtcgacct cgcgcccgac gaggggcagg acataatgcg
     4141 ccccctcctc gcggaggagg ggttcaagcc ccacgtcggc catgacctga gggtacgaca
     4201 cccaccgacc tcctgagtgc ttctccggct tcaggttcca gaagcgctgg cctgcgcatc
     4261 ttgcggccca ccgacctcgt cgatcggagg aacgtccgga atgtcaggaa tcgtatcctt
     4321 tcgcagcgac ccggtaaacg tgaaggtgga gtccttctcg tcgccagtgg catcgaccag
     4381 gacaaactca ccgctacgga gctctccgta gagaatcctt tcggagagca tgtcctcaat
     4441 ctcacgctgg atcgtgcgcc gcagaggccg cgcaccgagc accgggtcgt agcccttctt
     4501 ggccagcaag gccttggcgg ccatggtcaa ctcgaggccc atgtccttgt cgcgcaagcg
     4561 cttgtcgagc ttggcgatct cgaggtcaac gatggcgacg atctcgtcct gggtgagctg
     4621 cgggaagacg atgacgtcgt cgacacggtt gaggaactcg ggccggaagt ggttcttcag
     4681 ctcgtcggtg accttgttct tcatccgctc gtattccgag cgggtgtccg gtccggccga
     4741 gaagccgagc gaccccttgg tgatgtcccg ggtgccgagg ttggtcgtca tgatgatgac
     4801 ggtgttcttg aagtcg
//
LOCUS       DAOMZY010000278         4804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_16716_length_4804_cov_5.935144, whole genome shotgun sequence.
ACCESSION   DAOMZY010000278 DAOMZY010000000
VERSION     DAOMZY010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16716_length_4804_cov_5.935144"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PLK64_13555"
     CDS             complement(<1..399)
                     /locus_tag="PLK64_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80815.1"
                     /translation="MVTTLRGTDPGSVSRLGGLLRTTADALSGDLAALPATSPLAQLA
                     TVTADQVDAIGSALQVHAQELAEAAAAYSRLQERAAALGLIVTDWTVSEPFGLIGSDV
                     AARRGVERPQVQAQADRLASRLARGRAQLAR"
     gene            complement(393..752)
                     /locus_tag="PLK64_13560"
     CDS             complement(393..752)
                     /locus_tag="PLK64_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80816.1"
                     /translation="MPDGERPAHRSTRRDHGAPADVLAQVRQHALDASESLFAVDPML
                     GDPLVQRGVDEFVEQVVDALRVIAQLSQQRDPSWAGAEEHRAGSDVQHRATPDVERPG
                     GRDATRRAGSDMDRPRW"
     gene            876..>4804
                     /locus_tag="PLK64_13565"
     CDS             876..>4804
                     /locus_tag="PLK64_13565"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HPV80817.1"
                     /translation="MTTRNGLFTMTISLEQSRGNVLRSAAGLAQKGSGDPVEPLLTRY
                     YRHVSTEDLLARSPEDLLGAALSHLDLAHDRPVGTASVLVDNPTVESQGWSSGHTAIQ
                     IVIDDMPFLVDSVTMALTRRGLGVHLLVHPQLIVRRDARGVLEEVLDVDKAPSGEFGV
                     GVESWMHIEVDRMSNAADRDSLSAELQSLLGNVRDAVEDWPKMRERCVEISDGLRAAA
                     PAGIDGPEVAQGVRFLDWLAANHFTFLGYRQYVLGDDGGETVLHPVTGTGLGLLRYDA
                     AGSTAFARLSPPAQARARDRELLIITKANSRATVHRPVYLDYVGVKTFDAAGDVTGEH
                     RFLGLFAAAAYTASIMDIPIVGDRAREVLDRVGYSGDSHSGKDLLQVLESYPRDELFQ
                     TDADMLAEIAETVVHLHERIRTRLFLRKDVYGRFMSALVYLPRDRYNTAVRLRMEALL
                     RKAFAADSVDYTTRVSESPLAQVHFVLRVPKGAQVKDVDAAALESEIVDATRTWDEDL
                     LESARSEHGEELGARFAALYGKAFPEAYKEDFTPRVAIADVRRIEVLGTDDAVEFNMY
                     AEAGGPRDERRFKLYTRSPLSLTRVLPVFTHMGVEVTDERPYVLHRADGARVHVYDFG
                     LRATSAALWGEGAEREHCRARFQEAFSAIWRGRAESDGFNALVLQAGLAWREVLVLRA
                     LAKYLRQTQSTFSQDYVESALSANAGIARDLVELFAIRMDPTRYAGLPGAERTDAAQA
                     VVERITDSLVQVQSLDHDRIVRALLAIVLAATRTNHYQVDAEGEPKSYLSVKLDPKAL
                     PDLPAPRPMFEIWVYSPRVEGVHLRFGKVARGGLRWSDRREDFRTEVLGLVKAQMVKN
                     AVIVPTGSKGCFFPKQLPEPAVDRDAWLAEGIAAYQIFISGLLDVTDNRVGGEIVPPA
                     NVVRHDADDSYLVVAADKGTASFSDIANAKAQDYGFWLDDAFASGGSAGYDHKGMGIT
                     ARGAWESVKRHFREMGHDTQTQDFTCVGIGDMSGDVFGNGMLLSEHIRLVAAFDHRHV
                     FIDPTPDAASSFLERQRLFALPRSSWADYDRSLISAGGGVFERSAKAVPVSPEMAAVL
                     SIDPTITALTPAELMKAILLAKVDLLWNGGIGTYVKSIGETNAEVGDRANDSIRVNGR
                     ALRCRVVGEGGNLGCTQLGRIEAAQAGVRINTDAIDNSAGVDTSDHEVNIKILLGQLV
                     RDGDMTIKQRNTLLASMTDEVAAQVLRDNYEQNVLLGNARAQTLVMLPVHQRFIHYLE
                     QNGDLDRGLEYLPGDAEIVGRQADGRGLTSPEFSVLVAYSKLDLKAAL"
BASE COUNT          746 a         1708 c         1600 g          750 t
ORIGIN      
        1 gcgggcgagt tgggcccggc cccgagctag tcgggacgcc agccgatccg cctgggcctg
       61 cacctgcgga cgctcgacgc cgcgtcgggc ggccacgtcg gagccgatca ggccgaaggg
      121 ctccgagacc gtccaatcgg tgacgatcaa tcccagggcc gccgcgcgct cctgcagccg
      181 ggagtatgcc gccgccgcct cggcgagttc ttgggcatgg acctgcagcg ccgagccgat
      241 cgcatcgacc tggtcggcgg tgactgtggc caactgcgcc aatgggctgg ttgcaggcag
      301 ggccgcgaga tcgccggaga gggcgtccgc cgtcgtgcgc agcaggccgc ccagcctcga
      361 caccgagccg gggtccgttc cccgcagtgt cgtcaccatc ggggtcgatc catgtccgac
      421 ccggcgcgtc gtgtcgcgtc gcgcccacca ggccgctcta cgtccggcgt agcgcggtgt
      481 tgcacgtccg aaccggcgcg gtgctcctcg gccccggccc acgaagggtc ccgctgctgc
      541 gacagttggg cgatcacccg cagcgcatcc acgacctgct cgacgaactc gtccaccccg
      601 cgctgaacca gtgggtcgcc gagcatcggg tcgacggcga acaggctctc ggacgcgtca
      661 agggcgtgct ggcgcacctg cgcgaggaca tcggcaggcg cgccgtggtc gcggcgtgtg
      721 gaccggtgcg ctgggcgctc accatcgggc acgaacccac cctagacaac ctcgccccta
      781 ccccgttgcg gttgtcccca ggccgaaggt cccgcacaga ctcgaccctt tgtctcacct
      841 gccgatacgc tcggtgaggg cacaacgacg tgcccatgac gacgagaaac gggttgttca
      901 ccatgaccat ctcgcttgag cagtctcgcg gcaacgtgct gcgcagcgct gcgggcttgg
      961 ctcagaaggg ttccggggat cccgttgagc ctttgctgac gcgctactac cggcacgtgt
     1021 ccacggagga cctgctcgcg cggtccccgg aagacctgtt gggggcggcg ctgtcacacc
     1081 tcgacctcgc ccatgatcgg ccggtcggca cggcgagcgt gctcgtcgac aaccccacgg
     1141 tcgagtcgca gggctggagt tcggggcaca cggcgatcca gatcgtcatc gacgacatgc
     1201 cgttcctcgt cgactcggtg actatggcgc tgacccgccg gggcctgggc gtccacctgc
     1261 tcgttcaccc gcagctgatc gtccgccggg atgctcgtgg cgtgcttgag gaggtcctcg
     1321 acgtcgacaa ggctccgtct ggcgaattcg gtgtcggggt cgagtcgtgg atgcacatcg
     1381 aggtcgaccg gatgagcaac gcggccgacc gagactcgct gtccgccgag ttgcagagcc
     1441 tgctcggcaa cgtccgcgac gccgtcgagg actggccgaa gatgcgtgag cggtgcgtcg
     1501 agatcagcga cgggctgcgc gccgcagcgc cggcaggcat cgatggcccc gaggtggccc
     1561 aaggggtgcg attcctcgac tggctggccg ccaaccactt caccttcctc ggttaccgcc
     1621 agtacgtcct cggcgacgat ggcggcgaga cggtcctgca cccggtgacc ggaacgggcc
     1681 tcgggctgct gcgctacgac gccgccggat cgacggcctt cgcgcgcctc agcccgccgg
     1741 cccaggcccg ggcccgtgac cgcgagctgc tcatcatcac caaggcgaac tctcgcgcca
     1801 cggtccaccg gcccgtctac ctcgactacg tcggggtcaa gaccttcgac gcagcgggcg
     1861 acgtcaccgg cgagcaccgc ttcctcgggc tcttcgctgc cgcggcatac acggcctcga
     1921 tcatggacat ccccatcgtc ggcgaccggg cccgcgaggt cctggaccgg gtcggctaca
     1981 gcggtgacag ccactccgga aaggacctcc tccaggtcct cgagagctac ccccgcgacg
     2041 agctcttcca gaccgacgcc gacatgctgg ccgagatcgc cgagaccgtc gtccacctgc
     2101 acgagcggat ccgcacccga ctgttcctgc gcaaggacgt ctacggccgg ttcatgtcag
     2161 cgctggtcta cctcccgcgg gaccgctaca acacggcggt ccggttgcgc atggaagcgc
     2221 tgctgcgcaa ggcgttcgcc gccgacagcg tcgactacac gacccgggtg tccgagtcgc
     2281 cgctggccca ggtgcacttc gttctgcggg tgcccaaggg cgcccaggtg aaggacgtcg
     2341 acgctgcggc gttggagtcc gagatcgtcg acgccacccg cacgtgggac gaggacctcc
     2401 tggagagcgc ccgctccgag cacggcgagg agctcggcgc ccggttcgca gcgctctacg
     2461 gcaaagcctt ccccgaggcc tacaaagagg acttcacccc ccgggttgcc atcgcggacg
     2521 tccgtcggat cgaggtcctc ggcaccgacg acgccgtcga gttcaacatg tatgccgagg
     2581 cgggcgggcc gcgcgacgag cgccgcttca agctctacac gcgcagtccg ctctcgctga
     2641 cccgggtgct tccggtcttc acccacatgg gggtcgaggt caccgacgag cgtccctacg
     2701 tcctgcaccg ggccgatggt gcccgggtgc acgtctatga cttcgggctg cgggccacgt
     2761 cggccgcgtt gtggggcgag ggcgccgaac gcgagcactg ccgggcgcgc ttccaggagg
     2821 cgttcagcgc gatctggcgg ggtcgcgccg agtccgacgg tttcaacgcc ctcgtcctgc
     2881 aggcggggct ggcctggcgg gaggtcctcg tccttcgagc gttggccaag tacctgcgcc
     2941 agactcagtc gaccttctcc caggactacg tcgagtccgc gctgtcggcc aacgcgggca
     3001 tcgcccgcga tctcgtcgag ctgttcgcga tccgcatgga ccccacacgg tatgccggtc
     3061 tcccgggtgc cgagcgcacg gatgccgcgc aggctgtggt cgagcggatc actgactcgc
     3121 ttgtccaggt gcagagcctg gaccacgacc ggatcgtccg tgccctgctg gcgatcgtcc
     3181 tcgcggcgac ccggaccaac cactaccagg tcgacgccga gggcgaaccc aagagctacc
     3241 tgtcggtcaa gctcgacccg aaggccctgc ccgacctgcc cgcgcctcgc ccgatgttcg
     3301 agatctgggt ctacagcccc cgggtcgagg gcgttcacct gcgcttcggc aaggtcgcgc
     3361 gtggcggtct gcgctggagc gaccgacgcg aggacttccg caccgaggtg ctcgggctgg
     3421 tcaaggccca gatggtgaag aacgccgtca tcgtgccgac cggctccaag gggtgcttct
     3481 tcccgaagca acttcccgag ccggccgtcg atcgagacgc gtggctggcc gaggggatcg
     3541 cggcatacca gatcttcatc tccggtctgc tggacgtgac tgacaaccgc gtcgggggcg
     3601 agatcgtgcc gcccgccaac gtcgtgcggc acgacgccga cgactcctac ctcgtggtgg
     3661 ccgccgacaa gggcaccgcg tccttctccg acatcgccaa tgccaaggct caggactacg
     3721 ggttctggct cgacgacgcg ttcgcctccg gcggctcggc gggctatgac cacaagggca
     3781 tggggatcac cgcccgcgga gcctgggaat cggtcaagcg gcacttccgc gagatgggcc
     3841 acgacaccca gacgcaggac ttcacctgcg tcgggatcgg cgacatgtcc ggcgacgtct
     3901 tcggcaacgg catgttgttg tctgaacaca tccggctcgt cgcagccttc gaccaccggc
     3961 acgtgttcat cgaccccacc cccgacgccg cgagttcgtt cctggagcgg caacggttgt
     4021 tcgccctgcc gcgctcgtcg tgggccgact acgaccgttc gctgatcagc gccggcggag
     4081 gcgtcttcga gcggtcggcc aaggccgtgc cggtcagccc cgagatggcc gcggtactct
     4141 cgatcgatcc cacgatcacg gccctcaccc ccgcggagct gatgaaggcg atcctgctgg
     4201 ccaaggtcga cctgctgtgg aacggcggca tcggcaccta cgtcaagtcc atcggcgaga
     4261 ccaacgccga ggtgggcgac cgggccaacg actcgatccg cgtcaacggc cgcgcgctgc
     4321 gctgccgggt cgtcggcgaa ggcggcaacc tcgggtgcac ccagttgggc cgaatcgagg
     4381 ccgctcaggc gggggtgcgc atcaacaccg atgcgatcga caactccgca ggcgtcgata
     4441 ccagcgacca tgaggtcaat atcaagatcc tcctcgggca gttggtgcgt gacggcgata
     4501 tgacgatcaa gcagcgcaac acactgctgg cctcgatgac cgatgaggtg gcagctcagg
     4561 tcctgcggga caactacgag cagaacgtcc tgcttggcaa cgcccgcgct cagacgctcg
     4621 tgatgctgcc cgtccaccag cgcttcatcc actacctcga gcagaacggc gacctcgacc
     4681 ggggcctgga gtatctgccc ggcgacgccg agattgtcgg tcggcaggcc gacgggcggg
     4741 gcctgacctc gccggagttc tcggtcctcg tcgcctattc caaactcgac ctcaaggcgg
     4801 ccct
//
LOCUS       DAOMZY010000279         4801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_16727_length_4801_cov_4.097767, whole genome shotgun sequence.
ACCESSION   DAOMZY010000279 DAOMZY010000000
VERSION     DAOMZY010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16727_length_4801_cov_4.097767"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="PLK64_13570"
     CDS             complement(<1..838)
                     /locus_tag="PLK64_13570"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF014347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HPV80818.1"
                     /translation="MTIALSSLLATGVPPLDAAATAAADARQAQLTKPAGSMGRLESL
                     GSRLAGMYAVCPPPIPEPAVVAVFAGDHGVHAQGVSPWPQEVTVQMVANVAAGGAVVN
                     ALARQVGASVCVIDVGVAGALPPGVDAVGARIVDGTADFTTGPALTREQAEAAVLVGA
                     QTADRLIDEGARLLVTGDLGIGNTTASAALIAVFAGADPGAVTGPGAGSDARMLAHKI
                     AVIRAGIERHQPDPADPIGVLAAFGGAEHAALAGFLVAAAARRVPVILDGVIAGAAAL
                     AAG"
     gene            878..1420
                     /locus_tag="PLK64_13575"
     CDS             878..1420
                     /locus_tag="PLK64_13575"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HPV80819.1"
                     /translation="MSVTVVTGGVRSGKSRHAEALLAERDDVTYIATGQPADADADDA
                     EWRERVARHQAARPTTWTTVETLDLVPLLDMPGGPLLVECLGTWVTGLVDRAGLWDDL
                     PAAAALVEAEGAALAEALTRTSRDVVLVTNEVGWSLVPLTPSGRFFTDALGRVNTAVA
                     GVADHAVLVVLGRALTLGSL"
     gene            1417..2220
                     /locus_tag="PLK64_13580"
     CDS             1417..2220
                     /locus_tag="PLK64_13580"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HPV80820.1"
                     /translation="MSTGRDGWRLAVGTLTTIPVRPPSVVDRAVAGRAMLLAPLAALP
                     LALIPLLAHTAIRWLGMPPLLAAALTVAALALANRGLHVDGLADTADGLAASYDRDRA
                     LEVMRRGDVGPAGVATVVLVLLIQVVALADLLQLPSWDRPLLAALAVAASRHTLAWVC
                     GRAFPAARPEGLGAAVAGSVPSGAAAAAGLALAAVGAVAISVLAPTDTPWSSVGWYAA
                     PVTIAVAVVAALAVALRARSRLGGVTGDVLGAGVEISLAAALTVAVCLG"
     gene            2239..2922
                     /locus_tag="PLK64_13585"
     CDS             2239..2922
                     /locus_tag="PLK64_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011773045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HPV80821.1"
                     /translation="MYVPAHFAMTDDQVSAVLARIGSAELITHHSDTGLVATNLPFLH
                     DPSVGPQGALLGHVARNNRQWSDPVDGEALVIVTETDHYVSPAWLPSKAEHGKVVPTW
                     NYVTVHVYGELIAHDDIEWTREVVRRLTERHELGADQPWSVEDAPEGYIDAMLRAIVG
                     IELRITRIVAKAKMAQNKTPADVEAIASVLAAQGDSRGEDWLREISLPAAERRAALIA
                     EVGAQRERR"
     gene            complement(2919..4067)
                     /locus_tag="PLK64_13590"
     CDS             complement(2919..4067)
                     /locus_tag="PLK64_13590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HPV80822.1"
                     /translation="MRLAQALGVQEWMVHHRRHLHQHPEVGLDLPQTHDYVASVLRDL
                     GYEVEVHRAAGVTARVAGSAPDGTVSVLRADMDALPVTERVELSFRSLHEGAMHACGH
                     DLHMAMLLGAAQVLADHPPRRDTVLAFQPGEEADRGALPLLEHTNLGFAGPATAFAVH
                     VHALAPPGTILVRPGVFMAYGDWFTIELAGPGGHASQPHLVGNPVEAAGEITLRLRDC
                     VAEVGRGEAVVATVTESRSGNTVNVIPAEGRLRGTLRTLTPQTRTALIGAMHTLVADV
                     AEQHRLTGRLTIHEGYPAVVNDAGFINRISTHLGQTELADQVTPMPDPSMVIEDFAYF
                     LHRWPGAMVYLGACGPGNTSFNHADDVTYDESALAVGAALHLMAADGF"
     gene            complement(4105..>4801)
                     /locus_tag="PLK64_13595"
     CDS             complement(4105..>4801)
                     /locus_tag="PLK64_13595"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPV80823.1"
                     /translation="RSYLEHLRTAGIEASGVREIPGVDTGAAMILVDDLGENLIIVDP
                     GANSRLEPQSVEGSIRAHEPTVLLAQLEVPIAALEAAVAAVDARTIVVLNPAPMPADP
                     TLLAGVLRRVDVLVPNRRELGQFAGCEEPRNLQEVTRCLTSLPFHGTVVVTLGADGAV
                     VAEPGRSLVHVQAKVVEPVDTSGAGDAFCGALVVGLAAGADLADAVRRAVDVASESTL
                     VRGAQLAVSPTVP"
BASE COUNT          665 a         1688 c         1763 g          685 t
ORIGIN      
        1 gccccgccgc cagcgcagcc gccccggcga tcacgccatc gaggatcacc ggcacccgcc
       61 gagccgcagc cgcaacgagg aagcccgcga gcgcggcgtg ctcagcaccg ccgaaggcgg
      121 ccaggacacc gatcgggtcg gcgggatcgg gctggtgccg ctcgatcccc gcccggataa
      181 cggcgatctt gtgggccagc attcgggcat cgctgcccgc gcctgggccc gtgaccgcgc
      241 ccgggtcggc acctgcgaag accgcgatga gcgcagccga ggcggtggtg ttgccgatcc
      301 ccaggtcgcc ggtgaccaac aatcgagcgc cctcgtcgat gagccggtca gcggtctggg
      361 cgccgacgag cacggcagcc tccgcctgct cgcgggtgag ggccggtccg gtcgtgaagt
      421 cggccgtgcc gtcaacgatc cgcgcaccga ccgcgtcgac cccgggcggc aacgccccgg
      481 ccacgccgac gtcgatgaca cagacgctcg cccccacctg acgggccaac gcgttgacga
      541 ccgccccgcc cgcggcgacg ttggcgacca tctgcacggt cacctcctgc ggccacggcg
      601 acaccccttg ggcgtgcacc ccgtggtcgc cggcgaagac cgcgacgacg gccggttcgg
      661 ggatgggggg tgggcacacg gcatacatcc cggccagccg tgaccccagc gactcgagcc
      721 gccccatcga gccggccggt ttggtgagtt gcgcctgacg cgcatcggcg gcggcggtgg
      781 cggcggcgtc cagcggcggg acacccgtgg cgaggaggga gctcagagca atggtcacaa
      841 gatccatcct gcccgacgtc gggcatagcc tggtcgcgtg tcggtgacag tcgtgacggg
      901 tggggtgcgc agcggcaaga gccgccatgc cgaagcgctg cttgccgagc gcgacgacgt
      961 cacctatatc gccaccggcc aacccgccga cgccgacgcc gacgacgctg agtggcgcga
     1021 gcgggtcgcg cgccaccaag ccgcccgccc aacgacctgg acgaccgtcg agaccctcga
     1081 tctggtccca ctcctcgata tgccgggtgg tccccttctc gtcgaatgtc tcggcacgtg
     1141 ggtcaccggc ctggtcgacc gggccggcct ctgggacgac ctccccgcgg cggcggccct
     1201 cgtcgaggcc gagggcgcgg cgctggccga ggccctcacc cgcacgtcgc gcgacgtcgt
     1261 cctcgtcacc aacgaggtcg gttggtccct tgtcccgctc acgccgtcgg gccggttctt
     1321 caccgatgca ctcgggcgag tcaacacggc cgtagcgggg gtggccgacc acgctgtgct
     1381 cgtcgtgctg ggccgcgcgc tgacgttggg gtcgctgtga gcaccggccg cgacggctgg
     1441 cggctcgccg tgggcaccct gacgaccatc ccggtccggc caccgtcggt cgtcgatcgg
     1501 gccgtggccg gtcgcgccat gctgctcgct cccctcgccg ccctgccgct ggctctcatc
     1561 cccctcctcg cgcacacagc aatccgctgg ctcggtatgc cgcccctgct cgccgccgcg
     1621 ctcactgtcg ctgcgctcgc gctggccaac cggggcctgc acgtcgatgg gctggctgat
     1681 acggccgacg gcttggctgc ctcctacgac cgggaccggg cgcttgaggt catgcgccgc
     1741 ggcgacgtcg gcccggccgg ggtggcgacg gtcgtgctcg tcctgctcat ccaggtcgtc
     1801 gccctcgccg acctgctgca gttgccgagt tgggaccggc ccctgctggc ggcgctcgcg
     1861 gtggcggcgt cacggcatac cctcgcgtgg gtctgtgggc gcgccttccc ggcggctcgc
     1921 cccgagggcc tcggcgcggc cgtggccggg agcgtcccct cgggtgcggc ggctgcggcg
     1981 gggctcgcgc tggccgcggt gggggccgtc gcgatctccg tcctcgcccc caccgacacg
     2041 ccgtggtcgt cggtcggctg gtatgccgcc cccgtcacca tcgcggtggc tgtcgtcgcg
     2101 gcgctggcgg tggcgctgcg ggcccgctca cggctcggcg gcgtgacggg cgacgtgctc
     2161 ggcgccgggg tcgagatctc gctggcggcg gcgctcaccg tggcggtctg cctcggctga
     2221 acgtgcgatg gtggaggcgt gtacgttcct gcgcacttcg cgatgaccga cgatcaggtg
     2281 agcgcggtgc tggcccggat cgggtcggcc gaactcatca cccaccacag cgacacgggg
     2341 ctcgtcgcca cgaacctgcc attcctgcac gatcccagcg tcggcccgca gggcgcgttg
     2401 ctcggccacg tggcccggaa caaccggcag tggtccgacc cggtggacgg cgaggcgctg
     2461 gtcatcgtca ccgagaccga ccactacgtg agcccggcct ggctgccgtc gaaggccgag
     2521 cacggcaagg tcgttccgac ctggaactac gtcacggtcc atgtgtatgg cgagttgatc
     2581 gcccacgatg acatcgagtg gacgcgcgag gtggtgcgcc ggctcaccga gcggcacgaa
     2641 ctcggggcgg accagccgtg gtcggtcgag gatgcgcccg agggctacat cgacgcgatg
     2701 ctccgggcca ttgtcggtat cgaactgcgg atcacccgga tcgtcgccaa ggcgaagatg
     2761 gcccagaaca agacgcccgc cgatgtcgag gcgatcgcgt ctgtccttgc ggcacaaggg
     2821 gactcacggg gcgaagactg gctgcgcgag atctcgctgc cggcggccga gcgacgtgcg
     2881 gcactcatcg ccgaggtcgg cgcccagcga gagcggcgtt agaagccgtc ggctgccatg
     2941 aggtgcaggg cggctccgac ggccagtgcg ctctcgtcgt aggtgacgtc gtcggcgtgg
     3001 ttgaaggacg tgttgccggg cccgcaggca ccgaggtaga ccatggcgcc cggccaccgg
     3061 tgcaagaagt aggcgaagtc ctcgatgacc atcgacgggt ccggcatggg ggtgacctgg
     3121 tcggccaact cggtttggcc caggtgcgtg ctgatccggt tgatgaagcc ggcgtcgttg
     3181 acgaccgcgg ggtagccctc gtggatggtg aggcgtccgg tcagacgatg ctgctcggcg
     3241 acgtcagcca cgagggtgtg catcgcgccg atgagcgcgg tccgggtctg tggagtcagg
     3301 gtgcgcaggg tgccgcgaag gcggccttcg gctggaatca cgttgacggt gttgccgctc
     3361 cggctctcag tgacggtggc cacgaccgcc tcgccgcgtc caacctcggc cacgcagtcg
     3421 cgaagccgca gggtgatctc gcccgcggct tcgaccgggt tgcccacgag gtgcggctgg
     3481 ctggcatggc cgcctggccc ggcaagctcg atcgtgaacc agtcgccgta ggccatgaag
     3541 acaccgggac gcaccaggat ggtgccgggc ggcgcgagcg cgtgcacgtg gacggcgaac
     3601 gccgtggccg gaccggcgaa ccccagattg gtgtgttcaa ggagcgggag cgccccgcga
     3661 tcggcctctt ctcccggttg gaaggccaac acggtgtcgc ggcgcggcgg atggtcggcc
     3721 agcacctggg cggcgccgag gagcatggcc atgtgcaggt cgtgaccgca ggcgtgcatc
     3781 gcgccctcgt gaagtgagcg gaaggacagc tcgacccgct cggtgaccgg gagtgcgtcc
     3841 atgtcggccc gcaggaccga gacagtcccg tcgggcgccg agccggcgac gcgagcggtg
     3901 accccggccg cccggtggac ctcgacctcg tagccgaggt cgcgcaggac ggaggcgacg
     3961 tagtcgtggg tctgcgggag gtcgaggccg acctccggat gctggtgcag gtgacgacgg
     4021 tggtgcacca tccactcctg gactccgagc gcttgcgcaa gcctcatcgg gagaccagcc
     4081 ggtcggtggc gaacaggggt gacatcaggg caccgtgggt gacacagcca actgggctcc
     4141 acggacgagg gtgctctcgc tggccacgtc gacggcacga cggactgcgt cggcgaggtc
     4201 ggcgcctgcg gccaacccga ccacgagcgc tccgcagaaa gcgtcgcccg cgcctgaggt
     4261 gtccacgggc tccacgacct tggcctggac gtgaacgagc gacctccccg gctccgcgac
     4321 gaccgcacca tcagcgccaa gggtgaccac gacggttccg tggaacggca acgaggtcaa
     4381 acacctggtg acctcctgca ggttgcgcgg ctcctcgcac ccggcgaact ggccgagttc
     4441 ccggcggttg gggacgagga catcgacgcg gcgcagcacc ccggccagca gcgtcgggtc
     4501 ggctggcatg ggagcgggat tgagcaccac gatggtccgc gcgtccaccg ccgcgacggc
     4561 ggcctccagg gctgcgatcg ggacctcgag ttgggccagc aggacggtcg gctcgtgcgc
     4621 gcggatgctg ccctcgaccg actggggctc cagccgggag ttggcgccgg ggtcgacgat
     4681 gatgaggttc tcgccgaggt cgtcgacgag gatcatcgcg gctccggtgt cgacgccagg
     4741 gatctccctg accccgctgg cctcgatacc ggccgtgcgc agatgctcca ggtaactacg
     4801 c
//
LOCUS       DAOMZY010000280         4778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_16829_length_4778_cov_5.962736, whole genome shotgun sequence.
ACCESSION   DAOMZY010000280 DAOMZY010000000
VERSION     DAOMZY010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16829_length_4778_cov_5.962736"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="PLK64_13600"
     CDS             complement(<1..529)
                     /locus_tag="PLK64_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015937390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HPV80824.1"
                     /translation="MLAIVCPGQGSQAPGLLAPWLELPSVHDRMQWFTTVAGIDLLEH
                     GTESDADTIRDTAIAQPLIVASGLAALLELFPKPSEAFTQVSVGAGHSVGEITAAAGT
                     GVITAEQALVFVRERGRQMAKASAVTPTGMSAVVGGDAQEVAAAIAAQGLTAANMNTA
                     GQVVAALDAQWREHAV"
     gene            complement(609..1853)
                     /locus_tag="PLK64_13605"
     CDS             complement(609..1853)
                     /locus_tag="PLK64_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV80825.1"
                     /translation="MNSAVARRRPGPQTRRAIERGAGTLSDAALRRIETQHDWYRALP
                     PAERSWVGLVAQAGIAAFVAWLADESGTAPVTADVFGTAPRELTSAITLRQTLDLIRS
                     VVDVVEDEVHVLAVPGDEQAVREGVLRYSREIAFAAAQVYAQAAEARGAWDARLEALV
                     VDAVLRGEADDSMQSRATALGWGSVSDVTVVAGRTPSGSSASAVDAIRRAAARRQVEA
                     LTAVQGRRLVIILGRTTRPAEIVTAVADLFGDGPIVLGPTVPHLFAAGRSARAAINGL
                     AAADGWPQAPRPVSADELLPERVLVGDVHARRVLVERIATPLRAHEVLLDTATAYLDS
                     GGLESAARVLFVHPNTVRYRLGKIAELTGYDLTHPRDGYAVRLALALARLSGSPPAAR
                     RPVPTPAVPRDGAAGDGIPQSL"
     gene            complement(1935..3356)
                     /gene="argG"
                     /locus_tag="PLK64_13610"
     CDS             complement(1935..3356)
                     /gene="argG"
                     /locus_tag="PLK64_13610"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPV80826.1"
                     /translation="MSKVLTSLPVGERVGIAFSGGLDTSVAVAWMRDKGAIPCTYTAD
                     IGQYDEPDIDSVPGRAGAYGAELSRLVDCRAALVEEGLSALACGAFHIRSGGKQYFNT
                     TPLGRAVTGTLLVRAMREDGVDIWGDGSTYKGNDIERFYRYGLIANPNLRIYKPWLDA
                     DFVNELGGRTEMSQWMAAHGLPYRDSVEKAYSTDANLWGATHEAKRLEHLDTSIEIVE
                     PIMGVRFWDAAVDVPSEDVTIAFVQGRPVSINGQEFTDAVALVHEANVIGGRHGLGMA
                     DQIENRIIEAKSRGIYEAPGMALLFIAYERLLSAIHNEDTIANYHYEGRRLGRLLYEG
                     RWFDPQSLMLRESLQRWVASAVTGEVVLRLRRGDDYSILDTRGPAFSYHPDKLSMERT
                     EDAAFGPLDRIGQLTMRNLDIADTRAKLELYAAQGQLLGGHAELIGRLEPGGAGAIAA
                     NPEVADEGDSEGLDRAAMESGTD"
     gene            complement(3497..4402)
                     /locus_tag="PLK64_13615"
     CDS             complement(3497..4402)
                     /locus_tag="PLK64_13615"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HPV80827.1"
                     /translation="MPEILPHAAVSRLLTERGRVASWGDLRTVADRGTLETLLAAGHL
                     QRVARGRYAAPVEIPARLAAERVAGVASHRTAAQHYGLAMKWVPSQPCVTVRRGRSLG
                     GRGKGLHLSYRSLPPSDIDGWITTPLRTVLDCAATLPFDEALTIADSALRDRKITRAQ
                     LVDRAATWPTRGRQRVLRVAAHADPRASGPIESVLRAICLEIPGLRVTAQVRVDRLGK
                     FLALVDLADERLRIVIEAEGFEFHGDREGFDRDCRRYDELVAKGWVVLRFTWEHIMRR
                     DRWVAQILAATVAHRMTMVEARLAC"
     gene            complement(4551..>4778)
                     /locus_tag="PLK64_13620"
     CDS             complement(4551..>4778)
                     /locus_tag="PLK64_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80828.1"
                     /translation="TKSELIFDITDRHFDRITRTDIETIATFPDHASAAAYLATVDAT
                     WAEALPAFEGARQDTGFTTVFTATAPRAATR"
BASE COUNT          748 a         1608 c         1703 g          719 t
ORIGIN      
        1 ccacagcgtg ctcgcgccac tgggcatcga gcgcggcgac aacctggccg gcggtgttca
       61 tgttggcggc ggtgagcccc tgggccgcaa tggccgccgc gacctcttgg gcgtccccgc
      121 cgacgaccgc gctcatcccg gtcggagtca ccgcgctcgc cttcgccatc tgacgaccgc
      181 gctcacgaac gaacaccagc gcctgctcgg cggtgatgac cccggtccct gccgcggccg
      241 tgatctcgcc gacggagtgc cccgccccga ccgacacctg ggtgaaggcc tcggagggct
      301 tcgggaagag ctcaagcagc gcggcgaggc cggacgcaac gatcagcggt tgggcgatcg
      361 ccgtgtcccg gatcgtgtcg gcgtcggact cggtgccgtg ttcgagcagg tcgatgccag
      421 cgacggtggt gaaccactgc atccggtcgt ggacggaggg cagctccaac cacggagcga
      481 gcagtcctgg ggcctgtgag ccctggccgg ggcagacgat cgcgagcacg tcatcagcgt
      541 gtcacgtagc cgtgatcgtc ttgtgacctg aaaccgacga agttccgggg ggcaggtttg
      601 gaggtttcct acaaagattg gggtatgccg tctccggccg ctccgtcgcg gggcaccgcg
      661 ggcgtcggaa ccggacggcg ggccgccgga ggggacccgg agaggcgggc gagggcgaga
      721 gccaggcgga cggcataccc gtcgcggggg tgggtgaggt cgtaaccggt gagttcggcg
      781 atcttcccga ggcggtagcg cacggtgttg ggatggacga agaggacccg ggccgccgac
      841 tcgagacccc cagagtcgag gtatgccgtg gccgtgtcga gcagcacttc gtgggcgcgc
      901 aacggcgtcg cgattcgttc gacgagcacc cgtcgggcgt gaacgtcacc gacgaggacg
      961 cgctccggga ggagctcgtc ggccgacacc ggccggggcg cctgcggcca cccatcggca
     1021 gcggcgagac cgttgatcgc ggcgcgcgca gagcgtccgg cggcgaagag gtggggcacc
     1081 gtcgggccga gaacgatcgg gccgtcgccg aagaggtcgg ccactgcggt gacgatctcg
     1141 gccgggcggg tcgtgcgccc gaggatgatg accagacgtc gaccctggac cgcggtgagc
     1201 gcttcgacct gccggcgcgc ggcggctcga cggatggcat cgaccgcgga tgcactactg
     1261 ccagaaggag ttcggcccgc gacgacggtg acgtccgaca ccgagcccca gcccagagcc
     1321 gtggcacgcg actgcatcga gtcgtcggct tccccgcgca ggacggcatc gacgacgagt
     1381 gcttccagcc gggcgtccca cgcgccgcga gcctcggcgg cttgggcata gacctgcgct
     1441 gcagcgaagg cgatctccct ggagtagcgc agcaccccct cgcgcaccgc ctgctcgtcg
     1501 cccggcacgg ccaacacatg gacctcgtcc tcgacgacgt cgacgacgct gcggatgagg
     1561 tcgagagtct ggcgcaaggt gatggccgac gtgagctccc gaggtgccgt gccgaagacg
     1621 tcagcggtca ccggcgcggt gcccgactcg tcagccagcc acgcgacgaa ggcggcgatc
     1681 ccggcctggg cgaccagacc gacccacgag cgttcggcag gcgggagcgc gcggtaccag
     1741 tcgtgctggg tctcgatcct gcgtaatgcg gcatccgaga gggtgccggc cccacgctcg
     1801 atcgcgcgcc gggtctgtgg ccccgggcgg cgacgggcaa cggcgctgtt cacgccagca
     1861 tcctatttgt atgccgccca caaccgtcgt ttagtcggtg gccagttgct tcgctgcgcg
     1921 gcgtcgtcgc tcagtcagtc ggtgccactc tccatggccg cgcggtccaa gccctccgag
     1981 tcgccttcgt cggccacctc ggggtttgcg gcaatcgcac cagcgccgcc cggctccaac
     2041 cggccgatga gctcggcgtg accgccgagc aactggccct gcgcggcata cagctccagt
     2101 ttggcgcgcg tgtctgcgat gtcgaggttg cgcatggtga gttggccgat ccggtccagc
     2161 gggccgaacg cggcgtcctc ggtgcgctcc atcgacaact tgtcggggtg gtaggagaac
     2221 gccggcccac gcgtgtcgag gatcgagtag tcgtcgccgc gacgcagccg cagcacgacc
     2281 tcaccggtca ccgcagaggc gacccaccgc tgcagcgact cgcgaagcat gagcgactgg
     2341 gggtcgaacc aacgcccttc gtagagcagc cgcccaagcc gccgcccttc gtagtggtag
     2401 ttggcgatgg tgtcctcgtt gtggatcgcc gacagcagcc gctcgtaggc gatgaacaac
     2461 agcgccatgc cgggggcctc atagatcccg cggctcttgg cctcgatgat ccggttctcg
     2521 atctggtcgg ccatgccgag cccgtggcgg ccaccgatga cgttggcctc gtggaccaga
     2581 gccaccgcgt cggtgaactc ctgcccgttg atcgacacgg ggcgcccctg gacgaaggcg
     2641 atcgtgacgt cctcggaggg cacgtcaacg gccgcgtccc agaaccgcac gcccatgatc
     2701 ggttcgacga tctcgatcga ggtgtccagg tgctccaggc gcttggcctc atgggtcgcg
     2761 ccccacaggt tggcgtcggt cgaataggcc ttctccaccg agtcgcggta gggcaggcca
     2821 tgcgccgcca tccactgcga catctcggtg cgcccaccga gctcgttgac gaagtcggcg
     2881 tccagccacg gcttgtagat ccgcaggttg gggttggcga tcaggccgta gcggtagaac
     2941 cgctcgatgt cgttgccctt gtaggtcgac ccgtcgcccc agatgtcgac gccgtcctcg
     3001 cgcatggcgc gcaccaacag agtgccggtg acggcgcggc ccaggggagt ggtgttgaag
     3061 tactgcttgc cgcccgagcg gatgtggaac gccccgcagg ccagcgccga cagcccctcc
     3121 tcgacgagcg cggcgcggca gtcgaccagc cgcgagagct ccgcgccata ggccccggcg
     3181 cgccccggca ccgagtcgat gtcgggctcg tcgtactgcc cgatgtcggc ggtgtacgtg
     3241 caggggatcg ctcccttgtc acgcatccag gcgacggcta ccgacgtgtc gagaccgccg
     3301 gagaatgcga tgccgacgcg ttcgccgacc gggagtgagg tgaggacctt ggacacgcgc
     3361 gcaagcctag aaccgcagcg gtatgccgcg tccccccgtc cgcgctccgg tcacccatca
     3421 ccctcatcac ctcagtgacc tcggaagccg gcgcacacgg cataccaatg gtcggggagc
     3481 cgaccaccgc tcgtgctcag caggcgaggc gggcttcgac cattgtcatt cggtgtgcga
     3541 cggtggctgc gaggatctgg gcgacccagc gatcacgacg catgatgtgc tcccaggtga
     3601 agcgcagcac cacccaccct ttggctacca gctcgtcgta gcggcggcaa tcgcggtcga
     3661 acccctccct gtcgccgtgg aattcgaaac cctcagcctc gatgacgatg cgcagccgct
     3721 cgtcggccag gtcgaccagc gcgaggaact tccccaaccg gtcaactcgc acctgcgcgg
     3781 tgaccctcaa acctgggatc tcgaggcaga tggcgcgcag gaccgattcg atgggcccgg
     3841 aagcccgagg gtcggcgtgg gcggccactc gcagcactcg ctgccgccca cgcgtcggcc
     3901 aggtggcggc gcggtcgacg agctgggcac gggtgatctt gcggtcgcga agcgcggagt
     3961 cggcgatcgt tagtgcctcg tcgaagggga gggtcgcggc gcagtccagg acggttcgca
     4021 gcggcgtggt gatccatccg tcgatgtcag aaggtggcag cgatcgatag gacaggtgca
     4081 gccccttgcc gcggccaccg agactccgcc cgcgtcggac ggtgacgcaa ggctggcttg
     4141 gcacccactt catggcgagc ccgtagtgct gcgccgccgt ccgatgggag gcaacgccgg
     4201 cgacgcgttc agcggcgagc cgggctggga tctcgacggg ggcggcatac cggccacgcg
     4261 cgacgcgctg gaggtgaccg gcggcgagga gggtttcgag ggtgccgcgg tcggcgacgg
     4321 ttcgaaggtc gccccacgac gccacccgac cgcgctcggt gagcagacgg ctcaccgcgg
     4381 cgtgggggag gatttcgggc acccgacaag agtgccgcgt gcgccagcgt tggcgctgac
     4441 gttatccaca gcctgcacac cgaagaccaa tggtctgggc ttccggggtt gaccacgctc
     4501 aacaggcgtg ccggccgcag accaatggca ttccgtctgc cggacgtcgg tcagcgagtg
     4561 gccgcccgag gtgcggtcgc cgtgaacacg gtcgtgaatc cggtgtcctg acgggcacct
     4621 tcgaaggcag gaagcgcctc cgcccacgtc gcgtccacgg tggcgaggta tgccgcagcg
     4681 ctcgcgtgat cggggaaggt ggcgatggtc tcgatgtccg ttcgggtgat ccggtcgaag
     4741 tgccggtctg taatgtcaaa aatcagttcg ctcttggt
//
LOCUS       DAOMZY010000281         4715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_17116_length_4715_cov_5.609871, whole genome shotgun sequence.
ACCESSION   DAOMZY010000281 DAOMZY010000000
VERSION     DAOMZY010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17116_length_4715_cov_5.609871"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..923
                     /locus_tag="PLK64_13625"
     CDS             147..923
                     /locus_tag="PLK64_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017834017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPV80829.1"
                     /translation="MSIHEGSWVPGTPCWVDISVPDLARSQAFYRGVLGWEFTPGSEE
                     FGGYTTALVNGRGAAGMSPPMPGADPAPTFWTVYLATADSAASEAAITAAGGQTIFPS
                     MQLADLGRMGVYADSTGAVFGTWESGSHTGYDVANEPGAVGWCEGMVGDFEAGKAFYA
                     AAFGFTYTDMSADGMSYAMFSVPGGEQPAGGLGLETERPPYWSVTFEVDGTDAAVARV
                     LENGGGVILEPFDFEYGRLAVVTGPDGEPFGIITSAPMPV"
     gene            937..1809
                     /locus_tag="PLK64_13630"
     CDS             937..1809
                     /locus_tag="PLK64_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HPV80830.1"
                     /translation="MPRAQSAGGIPRARRGRLRPPPHGDPVARQTGGVTVDDLGYAVP
                     LTSPIERVVSLVPSLTEVFAVSAPGLLVGATDWCVHPVSLDVVRVRGTKNPDVPRIAT
                     LAPDLVVASMEENREADVTTLRELGIPVWVTDIRTVDGALTSIERLLEIIEAPHCGWV
                     AEAREAWRDPVQVAGDSRLRAVVAIWRRPWMFLGSDTYAGDVLRRLGVDNVLEHSPWR
                     YPRVDFDELPAHDLVVLPDEPYRFTPDDGPEAFPGAATALVDGQALTWYGPRMVQAPT
                     LLAEQLGKSTTDAG"
     gene            1838..2545
                     /locus_tag="PLK64_13635"
     CDS             1838..2545
                     /locus_tag="PLK64_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80831.1"
                     /translation="MASLGSITRVTVPHPWLLAPFEGPRATAEALSLLPSAPWLLATS
                     AVAGHPVLAVPGLSGGNAWTVVLRRFLAIKGYAVHTPDKGTMRGAHATVVARLADRVS
                     ELASEAGQPVSIIGWSVGGAFTRQVALAQPESVRSLITLGAPLSGSWYPEGSAAGGPL
                     PVPTTAIYSRTDGTFDWRRCVQRPGPMTENVEIVSSHLGMATNPMTVHVVADRLGQAD
                     GRWRPYHSAWHLGEDDR"
     gene            complement(2566..>4715)
                     /gene="fdhF"
                     /locus_tag="PLK64_13640"
     CDS             complement(2566..>4715)
                     /gene="fdhF"
                     /locus_tag="PLK64_13640"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HPV80832.1"
                     /translation="AYGYAAHRDRVLEPMVRDHIDDPWQVVSWDTAIERVATGFRAIQ
                     ERYGFDAIGGISSSRCTNEEVYVVQKMVRAAFGNNNVDTCARVCHSPTGYGLGQAFGT
                     SAGTQDFESVDQADVILVIGANPTDAHPVFGSRIKRRLRNGAALIVADPRRIELVRSP
                     HVEAAYHLPVQPGTNVAFVNAMAHVIVTEGLTDGRFLTERCEDVTDYLAFIARPEHSP
                     EATEEVTGIPAPDLRAAARRYAAGPNSAIYYGLGVTEHSQGSTMVMGLANLAMLTGML
                     GRDGVGVNPLRGQNNVQGSCDMGSFPHELPGYRHVSGAEVRGIYERLWGVPLLAEPGL
                     RIPNMFDAALGGTFRGLYVQGEDIAQSDPNTRHVEAALRALDLVVVQDLFLNETARFA
                     HVFLPGTSFLEKDGTFTNAERRINRVRPVIANRVGKAEWEVVCDIATAMGYPMGYAAA
                     ADIMAEIAATTPTFAGVSFERLDAVGSLQWPVPDVESLGTPIMHVDSFARGRGRLVET
                     VYVPTTERTTRKFPLVLTTGRILSQYNVGAQTRRTENSTWHAADVLEIHPADAEVRGI
                     HSGDLVGLASRVGATTLRALVSDRMPPGVVYTTFHHPVTGANVVTTDNSDWATNCPEY
                     KVTAVEVTVATALDTPAPGHIVATSILSRADDIARQFAGLSPAEGAAAVATHLTRFWE
                     PRMLAELASRVRSGDPVDPLVVSALAEVESAAH"
BASE COUNT          668 a         1597 c         1708 g          742 t
ORIGIN      
        1 gccggtcagc cggtgcttcc ggcccggccc ttcgccactg ctctcgaccg gttgggcgtc
       61 gcgctggggt gcgcagccga ctgagcagag gcgtgtcggc gcccagagtt agggtgacct
      121 catcatcagc tcgcggaagg aaagctatga gcattcatga gggcagctgg gtgccgggga
      181 caccctgctg ggtcgacatc tctgttccag acctggcgcg cagccaggcg ttctatcggg
      241 gggtcctggg gtgggagttc accccgggca gcgaggagtt cggcggttac acaacggctc
      301 tcgtcaacgg ccggggggcc gctggcatgt cgccgccgat gccgggtgcc gacccggcgc
      361 cgacgttctg gacggtctat ctcgccacgg ctgactcggc cgcctccgag gcagcgatca
      421 cggccgccgg gggacagacg atcttcccct ccatgcagtt ggccgacctc ggccggatgg
      481 gtgtttacgc cgattcgacc ggcgcggtct tcggcacctg ggagtccggg tcgcacaccg
      541 gctacgacgt cgccaacgag ccgggcgcgg tcggctggtg tgagggcatg gtgggtgatt
      601 tcgaggcggg caaggcgttc tacgctgccg ccttcggctt cacctacacg gacatgtcgg
      661 ccgacggcat gtcctacgcg atgttctcgg tgcccggcgg cgaacagccc gccggtggcc
      721 tcggcctcga gaccgagcgc ccgccctatt ggtccgtgac cttcgaggtc gacggcaccg
      781 acgccgcggt cgcgcgggtc ctcgagaacg gcggcggggt catcctcgag cccttcgact
      841 tcgagtacgg acgcctggcg gtcgtcaccg gccccgacgg cgagccgttc ggcatcatca
      901 ccagcgcgcc gatgcccgtc tgacgagggc aggggtatgc cgcgcgcaca gtccgcgggc
      961 ggcatacctc gggctcggcg tgggcgactg cgccctccgc cgcacggcga ccccgttgcc
     1021 cggcagactg gcggggtgac cgtcgacgac ctcgggtatg ccgtgcccct cacctcaccg
     1081 atcgaacggg tggtgagcct cgtcccgtcg ctgaccgagg ttttcgctgt gagtgcgccg
     1141 ggcctgctgg tcggggcgac cgactggtgc gtccatccgg tgtccttgga cgtcgtgcgg
     1201 gtgcgcggca ccaagaaccc cgacgtgccg cggatcgcca cactcgcccc cgacctggtc
     1261 gtcgccagca tggaagagaa ccgcgaggcg gacgtcacca ccctgcggga gctcggaatc
     1321 ccggtgtggg tgaccgacat tcgcaccgtc gacggtgcct tgacctccat cgagcggttg
     1381 ttggagatca tcgaggcacc ccactgcggg tgggtggccg aggcccgcga ggcctggcgt
     1441 gacccggtcc aggtggccgg tgactcacgg ctgcgggcgg tcgtagcgat ctggcgccgg
     1501 ccgtggatgt tcctcggcag cgacacctac gccggcgacg tcctgcgtcg attgggcgtc
     1561 gacaacgtcc ttgagcacag tccctggcgt tacccgcgcg tggacttcga cgagttgccg
     1621 gcccacgacc tcgtcgtcct gcccgacgag ccctaccgct tcacgccgga cgacggtccg
     1681 gaggccttcc caggtgccgc gactgcgctt gtcgacggtc aggccctcac gtggtacggc
     1741 ccccgaatgg tccaggcgcc gaccctgctg gccgaacagt tgggcaagtc caccaccgac
     1801 gccggctagg ggttagggtc gggccggagg tggcggcatg gcgtcgttgg gaagcatcac
     1861 ccgggtgacc gtgccacatc cgtggctcct cgccccgttc gagggacccc gcgcgaccgc
     1921 cgaagcgctg tcgctgctgc cgtcggcgcc ctggctgctg gcgacgagcg ccgtggcggg
     1981 tcatccggtt ctcgccgttc ccgggctgtc ggggggcaac gcgtggaccg tggtcctgcg
     2041 caggttcctc gcgatcaagg ggtatgccgt gcacaccccg gacaagggca ccatgcgcgg
     2101 cgctcacgcc accgtggttg ctcgcctcgc cgaccgggtg agcgagctgg cgagtgaggc
     2161 aggccagccg gtgagcatca tcgggtggag cgtcggcggt gcgttcaccc ggcaggtggc
     2221 actcgcccag ccggagtcgg tgcgcagcct catcaccctg ggcgcgccgc tctcggggtc
     2281 ctggtatccc gaggggtcgg ctgcgggtgg cccgctgccg gtgccgacca ccgcgatcta
     2341 ttcacgcacc gacggcacct tcgactggcg ccgatgtgtg cagcggccgg gcccgatgac
     2401 cgagaacgtc gagatcgtct ccagccacct ggggatggcg accaacccga tgaccgtcca
     2461 cgttgtcgcc gaccgcctcg gtcaagccga cggacgctgg cgtccctacc actcggcctg
     2521 gcacttgggc gaggacgacc gctgagccgg tgaccaacgt ccggctcagt gcgcggccga
     2581 ctccacctcg gcaagagcgc tgacgacgag ggggtcgacc gggtcgccgg accgcacccg
     2641 cgaggccaac tcggcgagca tccgcggctc ccagaacctg gtgaggtggg tggccacggc
     2701 ggcggcgccc tcggccggcg agagccccgc gaactgccgg gcgatgtcgt cggcccggga
     2761 gaggatgctc gtggccacga tgtgcccagg cgccggtgtg tccaacgcgg tcgccacggt
     2821 cacctcgacc gccgtcacct tgtattcggg gcagttcgtc gcccagtcgg agttgtccgt
     2881 cgtcacgacg ttcgcgccgg tcaccgggtg gtggaaggtc gtgtagacca cgccgggagg
     2941 catccggtcg gacacaagcg cccgcagcgt cgtcgcgccg acgcgggaag ccagcccgac
     3001 gagatcaccg gagtggatgc cccgcacctc ggcgtccgcc ggatggatct ccaggacgtc
     3061 ggccgcatgc caggtcgagt tctcggtgcg acgggtctgg gctccgacgt tgtattggct
     3121 gaggatccgg ccggtggtca gcacgagcgg gaacttgcgg gtggtgcgct cggtcgtggg
     3181 gacgtagacg gtctcgacga gccggccccg acctcgggcg aacgagtcga cgtgcatgat
     3241 cggggtacct agcgactcga cgtcggggac gggccactgc agtgacccga cggcgtcgag
     3301 gcgctcgaac gagacgcctg cgaaggtcgg cgtcgtcgcg gcgatctccg ccatgatgtc
     3361 cgcggcggcg gcatacccca tggggtagcc catggccgtc gcgatgtcgc acacgacctc
     3421 ccactcggcc ttgccgactc ggttcgcgat gacggggcgg acccggttga tccggcgttc
     3481 ggcgttggtg aacgtcccgt ccttctcgag gaacgacgtt ccgggcagga agacgtgggc
     3541 gaagcgcgcc gtctcgttaa ggaacaggtc ctggacgacg accaagtcga gggcccgcag
     3601 cgcggcttcg acatggcggg tgttggggtc ggactgggcg atgtcttcgc cctggacata
     3661 gagccctcgg aacgttccgc cgagggccgc atcgaacatg ttcgggatcc gcaggcccgg
     3721 ctcggccaat agcggaacac cccacagccg ttcgtagatc cctcggacct cggccccgga
     3781 cacgtgccga tagccgggca actcgtgggg gaaggacccc atgtcgcacg acccctggac
     3841 gttgttctga ccgcgcagcg ggttgacccc caccccgtcg cggccgagca tcccggtgag
     3901 catcgcgagg ttggccaggc ccatgaccat cgtcgagccc tggctgtgct cggtcacccc
     3961 gaggccgtag tagatcgcgg agttggggcc cgcggcatac cggcgggcgg cggcacgcag
     4021 atcgggcgcc ggtatgccgg tgacctcctc cgttgcctcc ggcgagtgct cggggcgggc
     4081 gatgaaggcg aggtagtcgg tgacgtcctc acaccgctcg gtgaggaacc gtccgtcggt
     4141 cagcccctcg gtgacgatga cgtgggccat cgcgttgacg aaggcgacgt tcgtgcccgg
     4201 ctggacgggc aggtggtatg ccgcctcgac atgcggagag cggaccaact cgatccgtcg
     4261 ggggtcggcg acgatgagcg ccgctccgtt gcgcaggcgc cgcttgatcc gcgagccgaa
     4321 gaccgggtgg gcatcggtgg ggttggcgcc gatgacgagg atgacgtcgg cctggtcgac
     4381 cgactcgaag tcctgggtgc cggccgacgt gccgaacgcc tggccgagcc catacccggt
     4441 gggggagtgg cagacccgag cgcaggtgtc gacgttgttg ttgccgaagg cggcccgcac
     4501 catcttttga acgacgtaga cctcctcgtt ggtgcaccgc gacgaggaga tgcccccgat
     4561 ggcgtcgaag ccgtagcgct cctggatcgc ccggaaaccg gtggccaccc gctcgatcgc
     4621 cgtgtcccag gacacgacct gccaggggtc gtcgatgtgg tcgcggacca tcggctcgag
     4681 cacccggtcg cggtgggcgg catacccata ggcga
//
LOCUS       DAOMZY010000282         4703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_17172_length_4703_cov_6.431799, whole genome shotgun sequence.
ACCESSION   DAOMZY010000282 DAOMZY010000000
VERSION     DAOMZY010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17172_length_4703_cov_6.431799"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..1681)
                     /locus_tag="PLK64_13645"
     CDS             complement(53..1681)
                     /locus_tag="PLK64_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPV80833.1"
                     /translation="MTLSIATGETDLPLLHETISANLDATAARFPEHEALVDCATGKR
                     WTYAAFAADVDTLARGFAGSGVVKGDRVGIWAPNGAEWTLVQYATAKVGAILVNINPA
                     YRTHELQYVLNQAAIKVLVAAPSFKTSDYAGMIEEVRDEVATLGQVVLIGRESWDELW
                     AAAEQVSQAELEAIAAGLSPDDPINIQYTSGTTGFPKGATLSHVNILNNGFHVGEVCR
                     YTEADRICIPVPFYHCFGMVMGNLAATTHGACMVIPAPGFDPALTLAAVRDERCTSLY
                     GVPTMFVAEWALIEAGAASESDLATVRTGIMAGAPCPAELMKKVIAAGINEMTICYGM
                     TETSPVSTQTRTDDSFDRKVGTVGRCMPHLQIGIIDPSDGSFVERGGSGEFVTKGYSV
                     MLGYWNQPDKTAESIVDGWMRTGDLAVMDEDGYVQITGRIKDMVIRGGENIYPREIEE
                     FLYTHPDILDAQVIGVPDPKYGEELCAWVRMKPGTTPVSAASIREFATGKLAHYKIPR
                     YVEIVEDFPMTVTGKVRKVEMRELSAAHLGLDAH"
     gene            2109..3422
                     /locus_tag="PLK64_13650"
     CDS             2109..3422
                     /locus_tag="PLK64_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HPV80834.1"
                     /translation="MDTGDTAWLLVSSALVLLMAPGLALFYGGMVRSKSVLNMMMMTF
                     GALAVILVLWTLVGYSLAFGDDIGGGVLGNPLQYAGLGQLLSPEADVVGTVPAILFAV
                     FQGLFCVITGALVSGAIADRARFGAWLVFIGAWTLLVYAPVAHWVFDFSAGDHVGGWL
                     ANQVGVIDFAGGTAVEICSGASGLALALVLGKRIGFGRDPMRPHNLTLVMLGAGLLWF
                     GWFGFNAGSALGAGHKAAVVFVTTLLAGAAGTLGWLVLERFRDGKATSLGAASGMVAG
                     LVAITPSGGSLTPIGALLMGGVAGVVCAAAVGLKFRFGYDDSLDVVGVHLVGGLIGTL
                     GIGLLGTSAAPTAVDGLFYGGGVTQLGKQAIGAAVVLVYAFVVSGALGYAVERLIGFR
                     LIPEHEVSGIDLAIHGETAYDFHTVTGGRAGGHGILRSLSHPEED"
     gene            3424..3762
                     /locus_tag="PLK64_13655"
     CDS             3424..3762
                     /locus_tag="PLK64_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HPV80835.1"
                     /translation="MKLVTAVVKPHKWDEVRAALETVGVAGMTVSEVSGSGRQKGHTE
                     VYRGAEYDVTLVPKVRIEVLVDDHDVDDIVRTIVSAARTGRIGDGKVWVVPVDSVVRV
                     RTGERDEDAL"
     gene            complement(3784..>4703)
                     /gene="maeA"
                     /locus_tag="PLK64_13660"
     CDS             complement(3784..>4703)
                     /gene="maeA"
                     /locus_tag="PLK64_13660"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxaloacetate-decarboxylating malate
                     dehydrogenase"
                     /protein_id="HPV80836.1"
                     /translation="YRHTHCTFNDDIQGTAAVALAAVLAGVNITGMPLADHRIVIFGG
                     GTAGIGIADLLRDELIRAGVSSEDAVAQFYALGSKGLFVDDMPMLREFQRPYARSRAE
                     VDQWNLVDGSTRGIRLADVVRNVRPTIMIGTSTMGGAFTKAIVKDMAAHCERPIIMPL
                     SNPTSRAEATAADLVEWTDGHALIATGSPFDPVDHREVRHFIAQANNALIFPGLGLGV
                     AACQATRVTDRMIAAAAEALAARVNTYRPGAALLPSMSELRPVSSHVALAVAAAAAEE
                     GVARQPLTDPINEIYSRMWQPAYSPVEVV"
BASE COUNT          674 a         1599 c         1622 g          808 t
ORIGIN      
        1 ccaccgctcg cgcagtgcct tcagcgtggc cacgtcaccc ccgatttcgc gctcagtggg
       61 cgtcgaggcc caggtgcgcg gccgagagtt cccgcatctc gaccttgcgg accttgccgg
      121 tcacggtcat ggggaagtcc tcgacgatct cgacatagcg ggggatcttg tagtgggcga
      181 gcttcccggt ggcgaactcg cggatcgacg ccgccgacac cggggtcgtt ccgggcttca
      241 tccgcaccca ggcgcacagt tcctcgccgt acttcgggtc gggcacgccg atgacctgag
      301 cgtcgaggat gtccgggtgg gtgtagagga actcctcgat ctcgcgcggg tagatgttct
      361 ccccgccgcg gatgaccatg tccttgatcc gaccggtgat ctggacatag ccgtcctcgt
      421 ccatcaccgc caggtcgccc gtgcgcatcc atccgtcgac gatcgactcg gcggtcttgt
      481 cgggctggtt ccaatagccg agcatcactg aatagccctt ggtgacaaac tcaccggacc
      541 cgccgcgctc gacgaacgac ccgtcgctcg ggtcgatgat cccgatctgc aggtgcggca
      601 tacaccgccc gacggtcccc accttgcgat cgaacgagtc gtcggtgcga gtctgggtcg
      661 acaccggcga ggtctcggtc attccgtagc agatcgtcat ctcgttgata ccggcggcga
      721 tgaccttctt catcaactcg gccgggcacg gcgctcccgc catgatcccg gtgcgcacgg
      781 tcgccaggtc gctctcgctc gcggcaccgg cctcgatgag cgcccattcg gcgacgaaca
      841 tcgtcggcac gccatagagg ctcgtgcatc gctcgtcacg caccgcggcg agggtgagcg
      901 ccgggtcgaa gcccggcgcc gggatgacca tgcacgcccc atgggtggtc gccgcgaggt
      961 tgcccatgac catgccgaag cagtgataga agggcacggg aatgcagatc cggtcggcct
     1021 cggtgtagcg gcagacctcg cccacgtgga acccgttgtt gaggatgttg acgtggctga
     1081 gcgtcgcacc cttggggaat cccgtggtgc ccgaggtgta ttggatgttg atcgggtcgt
     1141 cgggcgagag ccccgccgcg atcgcctcca actccgcctg gctgacctgc tcggctgccg
     1201 cccacaactc gtcccaggac tcgcgcccga tgagaacgac ctgcccaagg gtcgccacct
     1261 cgtcacggac ctcctcgatc atccccgcat agtcgctcgt cttgaacgac ggagccgcga
     1321 caaggacctt gatcgccgcc tggttgagga cgtactgcaa ctcgtgggtc cgataggccg
     1381 ggttgatgtt gacgaggatc gcaccgacct tggccgttgc gtattgcacg agcgtccact
     1441 cggcgccgtt gggcgcccag atcccgaccc ggtcgccctt gacgacccca ctgccggcga
     1501 acccgcgggc caacgtgtcg acgtcggcgg cgaacgcggc atacgtccag cgcttgcccg
     1561 ttgcgcagtc gaccaacgcc tcgtgttcgg ggaagcgggc ggccgtggcg tcgaggttgg
     1621 ccgagatggt ctcgtgcagg agcgggaggt cggtttcgcc ggtcgcgatc gacagggtca
     1681 tgggagcggc ctttcgtccg aggtgtccag ggcagtccat cgcccaaggg agtcaaacgc
     1741 aacccgacta tggtcacctt tgccgcatgg cggcatacgg cgcaggtatg ccgcgtcgct
     1801 gccgacgttg tctgcgtaag tcatctaccg gctgttcggg aatccgcagc gccaggcacg
     1861 ctgcccgggc taagcgaggg cccgacggtg tgcgttggac ggggtgatca ccccactgat
     1921 cgcactccgt ggcgcactcg ccggaggtgg gaccgtccag gcgaaccaga ttcggaccgg
     1981 ggccctggcg cggacgggac tgagcggcat acctggagcg cgctaccgtg ggcgcgccca
     2041 acccgcccct gcccgcaagg gggaccgccg tgagcacgtt gcccgccttc accacactcg
     2101 tcaccgagat cgacaccggc gacaccgcct ggttgctggt ctccagcgcc ctcgtgctgc
     2161 tcatggcacc cggtctggcc ctgttctacg gcgggatggt tcgctccaag agcgtcctca
     2221 acatgatgat gatgaccttc ggcgcgctgg cggtcatcct cgtcctctgg acgctcgtgg
     2281 gctactcact cgccttcggg gacgacatcg gtgggggggt gctcggcaac ccgctgcagt
     2341 atgccggtct cggccaactg ctctcccccg aggctgacgt cgtgggcacc gtccccgcga
     2401 tcctcttcgc ggtcttccag ggcttgttct gtgtcatcac cggcgccctc gtctccgggg
     2461 cgatcgccga ccgggcccgg ttcggtgcct ggttggtctt catcggcgcc tggaccctgc
     2521 tggtctatgc gccagtggcc cactgggtgt tcgacttctc cgccggtgac catgtcggcg
     2581 gctggctggc caaccaggtc ggggtcatcg acttcgccgg cggcacggca gtcgagatct
     2641 gttcgggcgc ttccggattg gccctcgctc tggtgctcgg caagcggatc ggcttcggcc
     2701 gcgacccgat gcgcccgcac aacctcaccc tcgtcatgct cggcgccggg ttgctgtggt
     2761 tcggctggtt cggcttcaac gccggctcgg cgctgggggc cgggcacaag gccgcggtcg
     2821 tcttcgtgac gacgctgctg gccggagcgg cgggcaccct cggctggctc gtgctcgaac
     2881 gcttccgcga cggcaaggcg acctcgctcg gcgccgcgtc cggcatggtc gctggcctcg
     2941 tggcgatcac cccgtcgggt ggctccctca ccccgatcgg cgcgctcctc atgggcggcg
     3001 tggccggcgt tgtctgcgcg gcggccgtcg ggctgaagtt ccgcttcggc tacgacgact
     3061 cgctcgacgt cgtcggtgtc cacctcgtcg gtggcctcat cggcactctg ggcattggcc
     3121 tgctcggcac ctcggccgcc cccaccgccg tcgacgggct gttctatggc ggcggggtca
     3181 cccaactggg caagcaggcg atcggcgccg ccgtcgtcct cgtctacgcc ttcgtcgtct
     3241 ccggggccct ggggtatgcc gtggagcgcc tcatcggctt ccggctcatc cccgagcacg
     3301 aggtgagcgg catcgacctg gccatccatg gcgagaccgc ctacgacttc cataccgtga
     3361 cgggcgggcg cgccggtgga cacggcatac tccgctcgct gtcccatcca gaggaggact
     3421 gacgtgaagc tcgtcaccgc ggtggtcaag ccgcacaagt gggacgaggt gcgcgccgcc
     3481 ctcgaaaccg tcggcgtggc cgggatgacc gtgtcggagg tgagcgggtc gggccggcag
     3541 aagggccaca ccgaggtcta tcgcggcgcg gaatatgacg tcacgctcgt gccgaaggtg
     3601 cgcatcgagg tgctcgtcga tgaccacgac gtggacgaca tcgtccgcac catcgtcagc
     3661 gccgcacgca ccggccggat cggcgacggc aaggtgtggg tcgtgcccgt cgacagcgtc
     3721 gtgcgggtgc gcaccggcga gcgcgacgag gacgcgctct agaggcgcgt cggactgccg
     3781 aactcagacg acctcgaccg gggagtaggc cggctgccac atgcggctgt agatctcgtt
     3841 gatcgggtcg gtgaggggtt ggcgggcgac gccctcctcg gcggcagcgg cggccacggc
     3901 gagcgcgacg tgcgaggaga ccggccgaag ctcactcatc gacggcagca acgcggcacc
     3961 cgggcgatag gtgttgaccc gcgcggcgag agcctccgcg gcggccgcga tcatccggtc
     4021 ggtcacccgg gtcgcttggc acgcggcgac cccgagcccg agcccaggga agatcagggc
     4081 gttgttcgcc tgggcgatga agtggcgcac ctcgcggtgg tcgacagggt cgaaggggct
     4141 gccggtggcg atgagggcgt ggccgtcggt ccactcaacg aggtcggcgg ccgtggcctc
     4201 ggcgcgggaa gtggggttgg acagcggcat gatgatcggc cgttcgcagt gcgccgccat
     4261 gtccttgacg atggctttgg tgaaggcccc gcccatcgtc gaggtcccga tcatgatcgt
     4321 cgggcggacg ttgcgcacga cgtcggccaa ccggatcccg cgcgtcgagc cgtcgacgag
     4381 gttccactgg tcgacctcgg cccgggaccg ggcatagggc cgctggaact cccggagcat
     4441 gggcatgtcg tcgacgaaca gccccttgct ccccagagcg tagaactggg cgaccgcatc
     4501 ctcgctcgac accccggcac ggatgagttc gtcgcgcagc aggtcggcga tgccgatccc
     4561 ggccgtcccg ccaccgaaga tgacgatccg gtggtccgcg agcggcatac cggtgatgtt
     4621 cactcccgcg aggacagcgg cgagagcaac cgcggccgtc ccctggatgt cgtcgttgaa
     4681 ggtgcagtgg gtatgccggt act
//
LOCUS       DAOMZY010000283         4694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_17221_length_4694_cov_5.904074, whole genome shotgun sequence.
ACCESSION   DAOMZY010000283 DAOMZY010000000
VERSION     DAOMZY010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17221_length_4694_cov_5.904074"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="PLK64_13665"
     CDS             complement(<1..824)
                     /locus_tag="PLK64_13665"
                     /inference="COORDINATES: protein motif:HMM:NF018479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HPV80837.1"
                     /translation="MGMPSHRLRPMTGRDPSERGRASSTLELLFDLTVVIGLGTAAGQ
                     LAHYLAEGHATVAVIGFLMSAFAVVWAWMNWSWFASAYDTDDWAFRLLTLVQMVGALI
                     LSLGVPAVFSSFESGGQIDNQTMVSGYVVMRVPMIIQWLRAARSDPAHRGICLVYAQT
                     FAAAQTGWVIVAIAPLTFWPSLLLALALASTELLGPILLSRRHGGTPWHPHHIAERYG
                     LLIIIALGEGLIGTMATLSVLFDASGWSVDFVVVGLAGVGLTFGIWWAYFLFPSGAL"
     gene            complement(919..1581)
                     /locus_tag="PLK64_13670"
     CDS             complement(919..1581)
                     /locus_tag="PLK64_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80838.1"
                     /translation="MKDILPGRGRRAGVVTLALALVTSVTGLAGLAGCGSGGGSTTSA
                     AASTASGAAAQSPAVQSPTAAAPATTPAGGTGASNGSGSQPTWCQPPQGPMEILLGKG
                     TVFEITGDDKRCRFAATGGKRGVVDVSNLTAFDPTVTYEATRKGERAVRCNAPLVEPA
                     GVGEQSWLNATCFAQTFTINLYAAKAGTIYEFIYTGDPSIPVTAAQASTELLQVAKVI
                     LH"
     gene            complement(1632..>3438)
                     /locus_tag="PLK64_13675"
     CDS             complement(1632..>3438)
                     /locus_tag="PLK64_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80839.1"
                     /translation="WAVWDPQLRAESGVSEANLRRQVRLTSVDALNVEVAIPTFTVTL
                     GEPTAAADPQAPLTGRLALTGHQMVAAAGYAPGTDCRTVDPAIRSTTLPVTRQASATR
                     YWTPDAGIPQSREAVVNLTMPHLAAGEAVDICVFWYQLPSRSFDDIVVTAIERHTVVP
                     PSRAATEIAFVGVGRGGEAPSTPAGSLAAELARWTFQVIGNRTLTAACGFPSGNTPDP
                     VILHPVGDPGFLCRVEDSSYLFEPRDIELFTTDLAANAPEGRTRLRIDNRGCTPTDCG
                     TPRQARIELSDGRWMELVVRKVPFATAPLLPGGRIATEWSADQWRIDPSGFQGVAPTL
                     RTPVRQSAPALDTFGLRVVPTPDSPTDSLDVHWQADRDVTLVVLGHPLVPASLAPTCT
                     DTWVEPTAVANSGTVTLPGLCSNTDYGLRVTVTDAATGASQVYDWLRGGARVDVLTGL
                     TFNLVGRTRARPVPPEFLVAWSVSVAEPTGSTSPTGVGTGAVEGTLAALSVRVGEHTL
                     VSRTDPATTCTRVAPGTTLASGFDGGQPLGQLRVAVSFTLVAPGTSGCAGPPGTTNFS
                     VQQDVIWDGRSEQTFRLSAPDGRTVALTVRPATRD"
     assembly_gap    3439..3448
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3449..4666)
                     /locus_tag="PLK64_13680"
     CDS             complement(<3449..4666)
                     /locus_tag="PLK64_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80840.1"
                     /translation="MSAAPAGAGRHRRVIGALVVLVMLVAGALVGRALRPATPSLPTA
                     YPVLPASSVTARDGRVSPVTAQASSPAGYAVAPAEYVASAAPPPAVAVGAGLTVGAGR
                     PSPNPGGAPDLRADPEAGVSGSAPPSSAPPAAPDLADEAALALVTERYDELTGATGAR
                     PTATGSTAPSSAPTTSGSSEGGPAYPGIPTDLSKVPLRAFTDPCVLDASSAACGRGLS
                     AIVLGIRAMPEFQTQWVGEIRRSDLYAGECARQFPTAPIDWASDTIFAAVVNQPVVAR
                     LDLQLNTPGVFGSLIRVSHRGAVVTSEAALPSADLIAAWIDAFERGAPTPGIPLCMKV
                     SRQTMEINRDGCLGEFNYNRWLDCSAPFHLSVHTGCSSTSVAPLCSQVGDLLGGREIP
                     QVRQGPFGFQYSYD"
BASE COUNT          741 a         1574 c         1703 g          666 t
ORIGIN      
        1 agggcgccgc tggggaagag gaagtaggcc caccagatgc cgaaggtcaa cccgactccg
       61 gcgagcccga cgacgacgaa gtcgaccgac cacccggatg cgtcgaacag caccgacagc
      121 gtggccatcg tgccgatgag gccctcgccg agggcgatga tgatgagcag cccgtaacgc
      181 tcggcgatgt gatgcggatg ccagggcgtc ccgccgtgtc gccggctgag gaggatcggc
      241 ccgagcagct ccgtgctggc gagagcaagc gcgagcaaca ggctgggcca gaacgtcagg
      301 ggagcgatag cgacgatcac ccacccggtt tgcgcggcgg cgaacgtctg ggcatagacc
      361 aggcagatgc cgcgatgggc cgggtcggac cgggcggcgc gcaaccactg gatgatcatc
      421 ggcacccgca tgacgacgta accgctcacc atggtctggt tgtcgatctg gccgccgctc
      481 tcgaacgacg agaaaacggc cggcaccccg agcgagagga tcagcgcgcc gaccatctgc
      541 acgagggtga gcagtcggaa cgcccagtcg tcggtgtcat aggccgacgc gaaccacgac
      601 cagttcatcc aggcccacac gaccgcgaac gccgacatga ggaagccgat caccgcgacc
      661 gtcgcgtgcc cctcggcgag gtagtgcgcc aattgtcctg cagcagtgcc taatccgatg
      721 acaacggtga ggtcgaaaag caactcgagc gtgctcgacg cccggccccg ctcactcggg
      781 tcgcggccgg tcatcgggcg caggcggtgc gacggcatac ccatccgtgc agtgtaagga
      841 gtggactcac cgctgctcga ggcgcgcggc ataccccctg ggtatgccgc gagccgccgc
      901 tcgtgggcgt ccccgtggtc agtgcaggat gaccttggcg acctgaagca gctcggtgct
      961 ggcctgggcg gccgtcaccg ggatcgacgg gtcgcccgtg tagatgaatt cgtagatcgt
     1021 gccggccttg gcggcataca ggttgatcgt gaaggtttgc gcgaaacacg tggcgttgag
     1081 ccaggactgc tctcccacgc ccgcgggctc gaccagcggg gcattgcagc ggacggctcg
     1141 ttcgcccttg cgggttgcct cgtaggtgac cgtggggtcg aacgcggtga ggttgctgac
     1201 gtccacgaca ccccgcttgc cgccggttgc cgcgaaccga caccgcttgt cgtcgccggt
     1261 gatctcgaag acggtgccct tgccgagcag gatctccatc gggccttgcg gaggctggca
     1321 ccaggtcggc tgactgcctg agccgttgct ggcaccggtt cctcccgcgg gggtcgttgc
     1381 aggcgccgct gccgtggggg actggaccgc tggggattga gcggccgcgc cgctggctgt
     1441 cgatgcggcg gccgaggtgg tcgacccgcc accggacccg caccccgcta ggccggccag
     1501 gccggtcacg ctggtgacga gcgcgagcgc gagggtcacg acgccggctc gccgaccacg
     1561 gcccggcagg atgtccttca tagcgttctc cttcggtgaa tttcttctga catcaaggga
     1621 attcgtttcg gtcaatcccg tgtcgcaggg cgcacggtga gcgcgacggt gcggccatcg
     1681 ggagcgctca gccggaacgt ctgttcgctg cgcccgtccc agatcacgtc ctgttgcacg
     1741 ctgaagttcg tggtgcccgg cggtccggca cagccgctgg tcccgggtgc cacgagagtg
     1801 aaggacacgg cgacccgcaa ctgacccagt ggctggccac cgtcgaaccc gctggcaagg
     1861 gtcgtgccgg gggcgactcg cgtgcaggtg gtcgccggat cggtgcggga caccagggtg
     1921 tgctcgccga cccggaccga gagggcagcc agggttccct cgaccgcacc cgtgccgaca
     1981 cccgtcgggg aagtcgaccc ggtcggctcg gcaacgctga ccgaccacgc gaccaggaac
     2041 tccggcggga caggccgggc tcgggtgcgt ccgacgaggt tgaaggtcag cccggtcagc
     2101 acatcgaccc tggctccacc ccggagccag tcatagacct gcgacgcccc ggtcgcagcg
     2161 tcggtgactg tcacgcgcag gccgtagtcg gtgttggaac acagcccagg cagcgtcacc
     2221 gtgcccgagt tggcgacggc ggtcggctcc acccaggtgt ccgtgcaggt cggggccagg
     2281 ctcgcgggga cgagcggatg gccgagaacg accagggtga cgtctcgatc ggcctgccag
     2341 tgcacgtcca acgagtcggt cgggctgtcc ggagtcggca cgacccgcag gccgaaggtg
     2401 tccaaggccg gtgcgctctg ccggactgga gtgcgcagcg tcggtgccac gccctggaat
     2461 cccgaggggt cgatgcgcca ctggtctgcc gaccactcag tggcgatccg accacccggc
     2521 agcagcggcg cagtggcgaa cggcaccttg cgcacgacca actccatcca gcggccgtcg
     2581 ctgagctcga tccgcgcctg ccggggggtc ccgcaatcgg tcggtgtgca gcctcggttg
     2641 tcgatccgca gccgggtgcg cccctccggc gcattggcgg ccaggtcagt ggtgaacagc
     2701 tcgatatccc gcggctcgaa caggtaactg ctgtcctcca cccgacacag gaacccgggg
     2761 tcaccgacgg ggtgcaggat gaccgggtcg ggggtgttgc ccgaggggaa tccgcacgcg
     2821 gccgtgaggg tccgattgcc gatcacctgg aaggtccagc gcgccagttc cgcggccagc
     2881 gaaccggcgg gcgtgctggg cgcctcaccg ccgcgaccca ccccgacgaa cgcgatctcg
     2941 gttgccgccc gagacggagg cacgacggta tgccgctcga tcgccgtcac gacgatgtcg
     3001 tcgaaggagc ggctcggcag ctggtaccag aagacgcaaa tgtcgacggc ctcgccggcg
     3061 gccaggtgcg gcatcgtcag gttgacgacg gcttcgcggc tctgcgggat gcctgcgtcc
     3121 ggagtccagt agcgagtcgc actcgcctgg cgggtcaccg gcaacgtcgt gctgcggatg
     3181 gccgggtcga cggtgcggca gtcggtcccg ggggcatagc cggccgcggc gaccatctga
     3241 tgtccggtca gcgcgagtcg cccggtgagc ggagcctgcg ggtcggctgc ggcggtgggc
     3301 tcgccgaggg tcacggtgaa ggtggggatg gccacctcga cgttgagggc gtccacggaa
     3361 gtcaaccgca cctggcgacg gaggttggcc tcggagaccc cggactccgc gcgcagctgc
     3421 gggtcccaca ccgcccatnn nnnnnnnngt cataggagta ttggaagccg aagggtcctt
     3481 gacggacctg cgggatctcg cgacctccga gcaggtcgcc cacctggctg cacagcggtg
     3541 cgaccgacgt ggacgagcac ccggtgtgca ccgacaggtg gaacggcgcc gagcaatcga
     3601 gccaccggtt gtagttgaac tcaccgaggc acccatctcg gttgatctcc atggtctggc
     3661 gggacacctt catgcacagc gggattcccg gcgtgggcgc cccacgttcg aacgcatcga
     3721 tccaggccgc gatcaggtcg gccgagggga gggcggcctc gctggtgacc actgcacccc
     3781 ggtgcgacac ccggatgagt gagccgaaga cgccaggagt gttgagttgc aggtccagcc
     3841 gtgcgacgac cggttggttg acgacggcag cgaagatcgt gtcgctcgcc caatcgatgg
     3901 gggctgtcgg gaactgccgg gcgcactcac cggcatacag gtcgctgcga cggatctcac
     3961 cgacccactg ggtctggaac tccggcatcg cccggatccc caggacaatc gccgacaggc
     4021 cccgaccgca ggccgcagac gaggcgtcca agacgcacgg gtcggtgaag gcccgcagcg
     4081 gaaccttgct caggtcagtc ggtatccccg gatacgccgg gcctccctca ctggagccac
     4141 tcgtcgtggg ggcgctggac ggcgcggtcg acccggtggc cgtcggacgc gccccggtgg
     4201 ctccggtcaa ttcgtcgtag cgttcggtga cgagcgccag tgccgcctcg tcggccaaat
     4261 cgggtgccgc aggcggggcg ctgctcggcg gggcactccc gctcaccccc gcctccggat
     4321 cggcccgcag atcaggagcg ccgccggggt tggggctggg gcgcccggcg ccgaccgtca
     4381 gcccggcacc gaccgcaacc gccggcggcg gtgcggcgct ggcgacgtac tccgcaggcg
     4441 cgacggcata ccctgcaggg ctgctcgcct gcgctgtgac gggcgagacc ctcccgtcgc
     4501 gcgcggtgac cgacgacgcc gggagcacgg ggtatgccgt gggcaggctc ggcgtcgcgg
     4561 ggcgcagcgc gcgcccgacc agggcgccag cgacgagcat gacgaggacg acgagcgcgc
     4621 cgatcacgcg gcggtgtcgc cccgctcctg cgggggctgc ggacacgggt tggcgggcag
     4681 gggtgcttgt catg
//
LOCUS       DAOMZY010000284         4681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_17277_length_4681_cov_5.955901, whole genome shotgun sequence.
ACCESSION   DAOMZY010000284 DAOMZY010000000
VERSION     DAOMZY010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17277_length_4681_cov_5.955901"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1761)
                     /gene="mshD"
                     /locus_tag="PLK64_13685"
     CDS             complement(<1..1761)
                     /gene="mshD"
                     /locus_tag="PLK64_13685"
                     /EC_number="2.3.1.189"
                     /inference="COORDINATES: protein motif:HMM:TIGR03448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035447 - mycothiol synthase activity
                     [Evidence IEA];
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol synthase"
                     /protein_id="HPV80841.1"
                     /translation="MPSHPLAATPLASAPLAVRSLRPERAEAVRGLVAAAEAADGAPP
                     LSEAFLLGLASTSGAGHLLLYAGSQLVGYAQLTDPDDPDSAAEIVVHPQHRREGIATA
                     LLSAMPSGVRIWAHGASAAATAFVKARGLTPVRDLHILGLTLDDSLPPAELPEGLVVR
                     PFRPGTDEQAWVAVNAAAFASHPEQGRLTVADLRARMAQPWFDPAGLLMVVPSRAGEG
                     PTVAGFHWTKVHDGPTDDPATRRGEVYAVAVHPAYQGRGLGRSMTLLGLHHLRETGIG
                     EAFLYVDGDNEAARRTYDSLGFQSLANETMFTHTPPVVSGTMVPMSASADARVESEAP
                     VPPLTEPLTAAPLGGRMPDSAAADEVEARPVVALDLPQERYLEREISWLQFNERVLQL
                     AMDETVPLIERAKFLAIFSSNLDEFFMVRVAGLKRRIATGLAVRSAAGLEPRELLDRI
                     QHAAQELMRMHSWVFWDQIRPALHEEGISIVRWGDLNEAERAPLHGLFREQIFPVLTP
                     LAVDPAHPFPYISGLSLNLAVVLVNPKTGAEHFARVKVPPVLPRFLRVFEPGDDHGTQ
                     RARFIPLEDVIAVHLDQLFTG"
     gene            complement(1790..2500)
                     /locus_tag="PLK64_13690"
     CDS             complement(1790..2500)
                     /locus_tag="PLK64_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPV80842.1"
                     /translation="MAMLLLLTNTVAPSAEVLPALALLGHQVRILPAEPAALVHAPPG
                     DAVLVDARRDLAHAKSLCRVLRATGISTPLMAVLTEGGLAALNADWGLDDVLLDTAGP
                     AEVDARLRLAFTRVETEEAQEAGPIRAGDLTIDEVSYSARFRGRALDLTYKEFELLKF
                     LAQHPGRVFSRAQLLQEVWGYDYFGGTRTVDVHVRRLRAKLGPEHETLIGTVRNVGYR
                     FVPERPRDTIVAEVDAGV"
     gene            2619..2876
                     /locus_tag="PLK64_13695"
     CDS             2619..2876
                     /locus_tag="PLK64_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HPV80843.1"
                     /translation="MPSGTVLLRYWAAARAAAGVDTEAVPVPVEGESVGPLLDAAVTR
                     HPELARVLKVASVLVDGRAADRDTRVAPAGVVEVLPPFAGG"
     gene            2893..3717
                     /locus_tag="PLK64_13700"
     CDS             2893..3717
                     /locus_tag="PLK64_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80844.1"
                     /translation="MSDPSAATSHRRGPHVGRFGDVDSTRLPGLVTVAALTTTGALAA
                     TSLAGPGLLAGLLGILALIVALGWPILSSPPALGQVQVGLAVGGLLIAATVGLTTGSH
                     RLVWLPVAVAIGMLVSFFLQLLRSDDRHGLTEAMSVVATGLACVGSGAALVPLILTEP
                     GVRFALIGLAGLAAGVVAELTGRMPRVGAKAVFVVMLAGGLGGWLAAVLLGSAGSAGL
                     GAGMLLASFSYSARRIFGAVPGSRETPGQVALGVATVLLPTVLLLALGALAKVYSG"
     gene            complement(3744..4127)
                     /locus_tag="PLK64_13705"
     CDS             complement(3744..4127)
                     /locus_tag="PLK64_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HPV80845.1"
                     /translation="MARSLVVKLTALGDTWPPPTAELERVAQALTVATTALASGIPTS
                     VWLTGEATRLATPGYAARLTLPHAAPFEDLVDLLLGAEVVTVCTQCAARRGLTEADLL
                     PGARIAGAAGFVEQALAEGAQALVY"
     gene            4171..4671
                     /locus_tag="PLK64_13710"
     CDS             4171..4671
                     /locus_tag="PLK64_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HPV80846.1"
                     /translation="MFELDVNLPERLRPLAWLIGRWEGAGVLGYPTIESMHYGQEIRC
                     WHDGRAFLQWEARAWILDSENGERVRPASIETGFWRPGEDGREVELLLAHPTGILEMY
                     YGQMEPAKITLRTDSVMRSPHAKEYNAATRLYGLVEGDLMYTMDMAAVGEPMTPHLSA
                     QLKRVG"
BASE COUNT          655 a         1564 c         1761 g          701 t
ORIGIN      
        1 gccggtgaac aactggtcga ggtggaccgc gatgacgtct tccaggggga tgaaccgggc
       61 ccgctgggtg ccgtggtcat cgccgggctc gaacacccga aggaaccggg gcagcaccgg
      121 cggcaccttg acccgggcga agtgctccgc accggtcttg gggttgacca ggacgacggc
      181 gaggttgagc gaaagaccag agatgtaggg gaacgggtgg gccgggtcga cggccagcgg
      241 ggtgagcacc gggaagatct gctcgcggaa cagcccgtgc aacggcgcgc gctcggcctc
      301 gttgaggtcg ccccagcgca cgatgctgat gccctcttcg tgcagcgccg gacggatctg
      361 gtcccagaag acccacgagt gcatccgcat gagttcttgt gccgcgtgct ggatccggtc
      421 gagcaactcg cgcggctcaa gcccggccgc cgagcggacg gcgaggcccg tggcgatgcg
      481 gcgcttgagc ccggcgaccc ggaccatgaa gaactcgtcg aggttgctgg agaagatcgc
      541 gaggaacttc gcccgttcga tgagggggac ggtctcgtcc atcgccaact gcagcacgcg
      601 ctcgttgaac tgcagccacg agatctcgcg ctcgaggtag cgctcctgag gcagatcgag
      661 ggcgaccacg ggccgcgcct cgacctcatc ggccgccgcg gagtcgggca tccgacctcc
      721 gagcggcgcc gcagtgaggg gctcggtcaa cggtgggacg ggtgcttcgc tctcgactcg
      781 cgcgtcggcg ctggcgctca tggggaccat cgttccacta acaacaggtg gcgtgtgcgt
      841 gaacatcgtc tcgttcgcga ggctctggaa ccccagcgaa tcgtaggtgc gacgggccgc
      901 ctcgttgtct ccgtcgacgt aaaggaacgc ttcgccgatc cccgtttccc gcaggtgatg
      961 cagccccagc agggtcatcg agcggcccag tccgcggcct tggtatgccg ggtgcacggc
     1021 cacggcatac acctcaccgc ggcgcgtggc tgggtcgtcg gtgggaccgt cgtggacctt
     1081 cgtccagtgg aagccggcga ccgtggggcc ctcgcccgcg cgggacggga cgaccatgag
     1141 tagcccggcc gggtcgaacc acggctgagc catccgggcg cgcaggtcgg cgaccgtcag
     1201 ccgaccctgc tcggggtggg aggcgaaggc agcggcgttg acggcgaccc aggcctgctc
     1261 gtcggtcccc ggccggaagg gacgcacgac gagcccctcg ggaagctcgg cgggcggcaa
     1321 cgagtcgtcg agagtgaggc cgaggatgtg caggtcgcgc accggcgtca ggcctcgcgc
     1381 cttgacgaac gccgttgcgg cggcgctggc cccgtgggcc cagattcgca ctcccgacgg
     1441 catggccgac aacagggccg tcgcaatgcc ctcgcgccgg tgctgggggt gaacgacgat
     1501 ctcggccgcg ctgtcggggt cgtcggggtc ggtgagctgg gcgtacccga cgagttgcga
     1561 accggcatac aggagcaggt gtccggcgcc cgaggtggag gccaacccga gcaggaaagc
     1621 ctcggagagc ggaggagcgc cgtcggcggc ctcggccgct gccacgagac cgcgaaccgc
     1681 ctcggcccgc tcagggcgca gggagcggac ggccaggggt gcggaggcga gcggtgttgc
     1741 ggcgaggggg tgcgacggca tacccgattc catcatgaac gggtcggtgt cagaccccgg
     1801 catccacctc ggcgacgatg gtgtcgcggg gacgctcagg gacgaagcgg taaccgacgt
     1861 tgcggaccgt cccgatgaga gtctcgtgct cgggcccgag cttggcgcgc aggcgtcgga
     1921 cgtggacgtc gaccgtgcgg gtgccgccga agtagtcgta gccccagacc tcctgcagca
     1981 actgggcgcg ggagaagacc cggcctgggt gctgcgcgag gaacttgagc aactcgaact
     2041 ctttgtaggt gaggtcgagg gcacgcccac ggaaccgcgc ggagtaactc acctcgtcga
     2101 tcgtcagatc gccggcgcgg atcggcccgg cctcctgcgc ctcctcggtc tcgactcgag
     2161 tgaacgccag ccgcagacgc gcgtcgacct cggccggacc tgccgtgtcg agcaggacgt
     2221 cgtccaggcc ccagtcggcg ttgagggcgg cgaggccgcc ttcggtgagg acggccatga
     2281 ggggggtgga gatcccggtg gcccgcagga cccgacacag cgacttggcg tgggcgaggt
     2341 cgcgacgggc gtcgacgagc acagcgtcgc cgggcggagc gtgcaccagc gcggccggct
     2401 cggccggcag gatgcgaacc tggtggccca acagggcgag cgccggcagc acctcagcgc
     2461 tgggcgccac ggtgttggtc agcagcaaca acatggccac agggtgagga taatcgaggc
     2521 gtccgacatg cctcgggtct gtcctgtgag tgagaccgct tgcataggct gtgccagtgg
     2581 tccgcgcgca ccgtcgcggg tggtcgaaag gggtcggtat gccgtccggc acagtgctgc
     2641 tgcgctactg ggctgccgct cgggcggctg cgggcgtcga caccgaagcc gtcccggtgc
     2701 ccgtcgaggg cgagtcggtc gggccgttgc tcgacgcggc cgtcacgcgt caccccgagc
     2761 tggcccgcgt cctgaaggtg gcctccgtgc tcgtcgacgg gcgggccgcc gaccgtgaca
     2821 cgcgggtggc tccggcgggg gtcgtcgagg tccttccacc gtttgcgggc gggtgatgcg
     2881 acgatgtcgg gcgtgagtga cccctccgcg gccaccagcc accgtcgtgg cccccacgtc
     2941 gggcggttcg gcgacgtcga ctcgacccgg ttgcccggcc tggtcaccgt tgctgccctg
     3001 acgacgaccg gggcgctggc ggcaacctcc ctggcggggc cagggctgct cgccgggctg
     3061 ctcggcatcc tcgcgctcat cgtcgccctg gggtggccga tcctcagcag cccacccgcg
     3121 ctggggcagg ttcaagtcgg cctcgccgtc ggcggtctgc ttattgcggc gacggtcggc
     3181 ctgacgaccg ggtcgcaccg actcgtctgg cttcccgtcg cggtggccat cgggatgctc
     3241 gtgtcgttct tcctgcaact gctgcgatcc gacgaccggc acggtctcac cgaggcgatg
     3301 tcggtggtcg cgaccgggct ggcgtgcgtc gggtccggcg cggccctcgt gcccctcatc
     3361 ctcaccgagc ctggcgtgcg gttcgccctc atcgggttgg ccggtctcgc tgccggagtg
     3421 gttgccgagc tcaccggtcg gatgccgcgc gtcggggcca aggcggtctt cgtcgtcatg
     3481 ctcgccggcg gtttgggcgg gtggctggct gcggtgctgc tcgggtcggc cgggtcggct
     3541 ggcctcggcg ccggcatgct cctcgcgtcc ttctcctact cggcccggcg catcttcggc
     3601 gccgtccccg gctctcggga gacccccgga caggtggccc tcggggtcgc gaccgtgctg
     3661 ctgcccaccg tgttgctcct cgcccttggt gcgctggcca aggtctattc cggctgacgt
     3721 ctgatcccgc tgggcgcggc tggtcagtag accagggcct gggctccctc ggcgagggcc
     3781 tgctcgacga accccgcagc cccggcgatc cgggcaccgg gcagcaggtc ggcttcggtc
     3841 aaaccccgcc gcgccgcgca ctgggtgcac accgtgacga cctcggcgcc gagcagcagg
     3901 tcgaccaggt cctcgaacgg cgctgcgtgc gggagggtga ggcgcgcggc atacccgggg
     3961 gtcgcgaggc gcgtcgcctc cccggtgagc cacacgcttg tcggtatgcc gctcgctagc
     4021 gccgtcgtcg cgaccgtcag cgcctgggcg acgcgttcga gctcggcggt cgggggcggc
     4081 caggtgtcgc cgagggcggt gagtttgacg acgagggaac gggccatggg gccagactac
     4141 gatcggggaa ccggtccgga aggggtcctc atgttcgagc tcgacgtcaa tctgcccgag
     4201 cggctgcggc ccctggcctg gctcatcggt cggtgggagg gcgccggagt gctgggctat
     4261 ccgaccatcg agtcgatgca ctacggccag gaaatccgct gctggcatga cgggcgagcc
     4321 ttcctgcaat gggaggcgcg ggcctggatc ctcgactccg agaacggcga gcgggtgcgg
     4381 ccggcatcga tcgagaccgg cttctggcgg ccgggcgagg acgggcgcga ggtcgagctg
     4441 ctgctggcgc acccgacggg gatcctcgag atgtactacg ggcagatgga gccggccaag
     4501 atcaccctgc gcaccgactc ggtgatgcgc agcccgcacg ccaaggagta caacgcggcg
     4561 acccggcttt atgggctggt cgagggcgac ctcatgtaca cgatggacat ggcagccgtc
     4621 ggcgagccga tgaccccgca cctctcggcc caactcaagc gcgtcggctg accgccgcct
     4681 t
//
LOCUS       DAOMZY010000285         4619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_17590_length_4619_cov_5.410824, whole genome shotgun sequence.
ACCESSION   DAOMZY010000285 DAOMZY010000000
VERSION     DAOMZY010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17590_length_4619_cov_5.410824"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..753
                     /gene="pyrE"
                     /locus_tag="PLK64_13715"
     CDS             199..753
                     /gene="pyrE"
                     /locus_tag="PLK64_13715"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPV80847.1"
                     /translation="MTDIAADRARLLEIVKAKAIVHGRVTLSSGREADYYVDLRRITL
                     DGEAAPLVGRVMRDLTSDLDFDAVGGLTLGADPVATSMLHAASAAGGRLDAFVVRKSG
                     KAHGLQQRIEGPSISGRRVLIVEDTSTTGASPLEAANAAREAGATVVAVATIADRATG
                     AAAVFAEAGLPYRFVYGLAELGLA"
     gene            789..1484
                     /locus_tag="PLK64_13720"
     CDS             789..1484
                     /locus_tag="PLK64_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPV80848.1"
                     /translation="MIPLIIIGVIVLGLVAWFFSAYNGLIKLRNLTQEAWAQIDVELK
                     RRHDLIGNLVETVKGYAAHERGTLEAVTQARAAAMAPGLSPAATAKSEAALTQALVRL
                     NAVSEAYPDLKANQNFSALQAELGATEDRIASARRYYNANVRELNTKVESIPTNFVAG
                     MASVTKAEYFELDGPAEREAPKVSFDPAGGSAGGSVGQAGQAAPLPPAQPAPSPIQDS
                     PAYPQTPPPPAAG"
     gene            complement(1517..2155)
                     /locus_tag="PLK64_13725"
     CDS             complement(1517..2155)
                     /locus_tag="PLK64_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HPV80849.1"
                     /translation="MPPALGPDWLDPNYLFETYGSGFFWLSLGIVFVECGLFFPFLPG
                     DSLLFAIGLFISGGKPHLPSLAIALPTLTVVAFLGNVCGYEIGRAMGPALYKRTGRIL
                     KQEYFDKTHAFFEKHGTKALVIGRFVPIVRTFITLVAGVSAMNRRTFFVWSAVGAVLW
                     VLSITLAGYFLGAAFPAIGKNLEIAVIAIIGVSLLPMVFEWWRHRRAAASAD"
     gene            2356..3246
                     /locus_tag="PLK64_13730"
     CDS             2356..3246
                     /locus_tag="PLK64_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPV80850.1"
                     /translation="MLRSRMLAVSGSALLLAGLAACGGSTSTGPTTSAVATKAPSASA
                     TTDACAIASLPLTTAGKFTIGTDDPAYEPWFKDGKPANGQGYEAAVAAAVAAKMGFAA
                     ADVQWVVVPFTNAITPGKKNFDVDINQVSISEERRKAVDFSSGYYDVAQAVITYKGSP
                     IDGKTAVADLAGAKLGAQVGTTSYKAITDQIKPTAAPAVFDTNDLAVQALKNKQIDGI
                     VADVPTAFYMTAAQLDNGVIVGQLPAAGGAQEQFGLVLTKGSGLTACVSKAVDALRAD
                     GTLAKLATQWLTNAGAPALK"
     gene            3315..4181
                     /locus_tag="PLK64_13735"
     CDS             3315..4181
                     /locus_tag="PLK64_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HPV80851.1"
                     /translation="MDAPGGLTDRWVPSALEQERRAYRRSRTVRSAAIASISTILVVG
                     ALGWVLTHSSGWPRVQETFFDWDKAVASFPAVLSGLWLNIRVLLVCAVLITVLGLTIA
                     VMRTLRGPVFFPLRLFGAAFTDLFRGLPLLLVLLLLGFGVPALGLDGLPQEAVFWGCA
                     ALVLSYSAYVAEVFRAGIESVHPSQRAAARSLGLTHTQSLRFVIVPQAVRRVLPPLLN
                     DFVSLQKDSGLIAVLGVVDAIRAAQIETARDFNFTPYVVAGVLFVCLTVPMARFTDWV
                     ARRQGFQGSGLV"
     gene            4222..>4619
                     /locus_tag="PLK64_13740"
     CDS             4222..>4619
                     /locus_tag="PLK64_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPV80852.1"
                     /translation="MSLLSVRGLRKSFGSNVVLDGFDLEVSAGECVVLIGASGSGKST
                     LLRCVNLLETVDDGVIHLGTEDITDPRVDPDRVRSRMGMVFQAYNLFPHLSVLDNITL
                     APMRVHGRPRADATAAARAILERVGLADKAS"
BASE COUNT          709 a         1679 c         1523 g          708 t
ORIGIN      
        1 cgatcccgag gaccgcgaga tcgtcgaggc cgacttcgcc gatgcgccca ccctggcctg
       61 acgcccggcg gacctggcgc cggctgcaat tgattggatg cgtatccggt cggacgtgcc
      121 gaccggttat acctcgaccc gtcggctgac tatcgcagcg gccacgcaca ggctgggcac
      181 gcggcatact ggctgcccgt gactgacatc gctgccgacc gggcccgcct gctggagatc
      241 gtcaaggcca aggccatcgt tcatggccga gtgaccctgt catcggggcg tgaggcggac
      301 tactacgtcg acctgcgccg gatcaccctc gacggcgagg ccgcccccct ggtcgggcgg
      361 gtcatgcgcg atctgaccag cgacctcgac ttcgacgccg tcggcgggct gaccctcggc
      421 gccgacccgg tcgcgacctc gatgctgcac gcggcatccg cagcaggcgg ccgcctcgac
      481 gccttcgtcg tccgcaagtc gggcaaggcc cacggcctgc agcagcggat cgaaggcccc
      541 tcgatcagcg gtcgtcgggt gctcatcgtc gaggacacct cgaccaccgg agcctcgccg
      601 ctggaggctg ccaacgccgc ccgcgaggcc ggcgcaaccg tcgtcgccgt cgccacgatc
      661 gccgaccggg cgaccggagc ggcggctgtt ttcgccgagg cggggctgcc ctatcgcttc
      721 gtctacgggc tcgccgagct cggactcgcc taggatcggg ctcaacccac cggctaaggg
      781 agcctcgcat gatcccgctc atcatcatcg gcgtcatcgt tctgggactc gtcgcctggt
      841 tcttcagcgc ctacaacggc ctcatcaagc tgcgcaacct cacccaggag gcctgggccc
      901 agatcgacgt cgagctcaag cgacggcacg acctcatcgg caacctcgtg gagaccgtca
      961 aggggtatgc cgcgcacgag cgaggcacgc tcgaagccgt cacccaggcc cgtgcggccg
     1021 cgatggcgcc ggggctgagc ccggccgcaa cggccaagtc ggaggccgcc ttgacccagg
     1081 ccctcgtgcg gctcaacgcc gtcagcgagg cctaccccga cctcaaggcc aaccagaact
     1141 tctcggcgct gcaggctgag ctcggcgcca cggaggaccg gatcgccagc gcccggcgct
     1201 actacaacgc caacgtccgc gagctcaaca ccaaggtcga gtcgatcccg acgaacttcg
     1261 tcgcaggcat ggcgtcggtg accaaggccg agtacttcga gttggacggc cccgccgagc
     1321 gcgaggcccc gaaggtgtcc ttcgacccgg ccggtggctc ggctggtggc tcagtcggtc
     1381 aggccggcca ggccgcgccg ctgccgcccg cccaaccggc gccctccccg atccaggact
     1441 caccggcata cccgcagacg cctccgccgc ccgcagccgg ctagccgccg gctagccggg
     1501 cttgccgccc gggcgctcag tcagctgacg cggcggcccg gcggtggcgc caccactcga
     1561 aaaccatcgg gagcagtgac accccgatga tggcgatcac cgcgatctcg aggttcttgc
     1621 cgatcgccgg gaacgccgcg ccaaggaagt agccggcgag cgtgatcgac agcacccaca
     1681 gcaccgcgcc gacggcgctc cacacgaaga aggtccgccg gttcatcgcg ctgacgccgg
     1741 cgacgagggt gatgaaggtc cgcacgatcg gtacgaaccg gccaatgacc agcgccttcg
     1801 tgccgtgctt ctcgaagaag gcgtgcgtct tgtcgaagta ctcctgcttg aggatccgcc
     1861 ccgtccgctt gtagagcgcc ggccccatcg cccgcccgat ctcatacccg cacacgttcc
     1921 cgaggaacgc tacgacggtg agcgtcggca gcgcgatcgc cagcgagggc aggtgtggct
     1981 tgccccccga gatgaacagc ccaatcgcga acagcagcga gtcgccgggc aggaatggga
     2041 agaacagccc gcactcgacg aacacaatgc cgagcgagag ccagaagaat ccgctcccgt
     2101 aggtctcgaa cagatagttc gggtcgagcc agtcggggcc gagggcagga ggcatcacga
     2161 ccccgagggt atgccgcccg cctggctgcc ccgtaatcct cacctgtgcg cgcgccgtgt
     2221 tcgcggacac cagcgcgggc cttccacaaa caacggacca agcaaccgcc gctccgatgg
     2281 ccccctacga tgacgggtgt cagccggggt cgggacgatc tcggcgcctt gaattcgatg
     2341 tcgaagggaa cgttcatgtt gcgctcgcga atgctcgccg tctccgggtc cgccctgttg
     2401 ctggccggcc tggccgcctg cggggggagc acctcgacgg gaccgacgac gtcggccgtt
     2461 gcgaccaagg ccccttcggc gagcgccacc accgatgcct gcgccattgc gtcgctgccg
     2521 ctgaccaccg cgggcaagtt caccatcgga accgacgacc cggcatacga gccctggttc
     2581 aaggacggga agccggccaa cgggcagggc tatgaggcgg ccgtcgccgc cgctgtggcc
     2641 gcgaagatgg gcttcgccgc cgccgacgtc cagtgggtcg tcgtgccgtt cacgaacgcc
     2701 atcacccccg gcaagaagaa cttcgacgtc gacatcaacc aggtgtcgat ctccgaggag
     2761 cgtcgcaagg cagtggactt ctcgtccggc tactacgacg tcgcccaagc cgtcatcact
     2821 tacaagggtt cgccgatcga cggcaagacg gctgtggccg acctcgccgg cgccaagctc
     2881 ggcgcccagg tgggcacgac ctcctacaag gcgatcaccg accagatcaa gccgacggcg
     2941 gccccggccg tgttcgacac caacgacctg gccgtccagg cgctgaagaa caagcagatc
     3001 gacggcatcg tcgcggacgt cccgaccgcc ttctacatga ccgcggcgca gttggacaac
     3061 ggcgtcatcg tcgggcagtt gccggcggct ggcggcgcgc aggagcagtt cgggctggtc
     3121 ctcaccaagg ggtccgggct gaccgcctgc gtctcgaagg cggtcgacgc actgcgggcc
     3181 gacggcaccc tggccaagct cgcgacgcag tggctcacca acgccggcgc cccggcgctc
     3241 aagtaaccac ccgcacacct caccacacgg cataccccct cgggatcttc gtggcgaacc
     3301 ctcccgacga gcgtgtggac gcacccggcg gtctgaccga ccgctgggtg ccgtccgcgc
     3361 tggagcagga gcgccgcgcc taccgccgct cgcggacggt gcggtcggcg gccatcgcat
     3421 cgatcagcac gatcctcgtc gtcggcgcac tcgggtgggt gctcacgcac tcgtccgggt
     3481 ggccgcgggt ccaggagacg ttcttcgatt gggacaaggc cgtcgcgtcg ttcccggcgg
     3541 tgctcagcgg gctatggctc aacatccggg tgctgctggt ctgcgcggtc ctcatcacgg
     3601 tgttgggcct gacgatcgcc gtcatgcgga ccctgcgcgg cccggtcttc ttccccttgc
     3661 ggctgttcgg ggcggcgttc accgacctgt tccgtggtct gccgctgctg ctcgtcctgc
     3721 tgctgctggg tttcggcgtg cccgcgttgg gtctggacgg gttgccgcag gaggcggtgt
     3781 tctggggctg cgcggcgttg gtgctcagct actccgccta tgtcgccgag gtgttccgcg
     3841 ccggcatcga gtcggtccat ccgtcgcagc gcgcggccgc ccggtcgctc ggcctgaccc
     3901 acacccagtc cctgcggttc gtcatcgtgc ctcaggcggt gcgccgggtc ttgccgccgt
     3961 tgctcaacga cttcgtgtcc ctgcagaagg actccgggct catcgcggtc ctcggcgtcg
     4021 tcgacgcgat ccgcgcggcc cagatcgaga cggcccgcga cttcaacttc acgccgtatg
     4081 tcgtcgccgg cgtcctgttc gtttgcctca ccgtccccat ggcacgcttc accgactggg
     4141 tggcccgccg ccagggcttc caggggagcg ggctggtatg aacggcttcg ccgtcggccg
     4201 ctcccgccac cgaggtatgc cgtgagcctc ctgtccgtcc gcgggttgcg gaagtcgttc
     4261 ggcagcaacg tcgtcctcga cggtttcgac ctggaggtgt cggccggcga atgcgtcgtc
     4321 ctcatcggcg cctccgggtc gggcaagtcg accctgttgc ggtgcgtcaa cctgttggag
     4381 accgtcgacg acggcgtcat ccacctcggc accgaggaca tcaccgaccc gcgggtcgac
     4441 cctgaccggg tgcggtcgcg gatggggatg gtctttcagg cctacaacct gttcccgcac
     4501 ctgagcgtcc tggacaacat caccctggcc ccgatgcggg tccacggccg acctcgggcc
     4561 gacgcgaccg ccgctgcccg ggcgatcctt gagcgggtcg ggctggccga caaggcgtc
//
LOCUS       DAOMZY010000286         4610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_17633_length_4610_cov_4.479693, whole genome shotgun sequence.
ACCESSION   DAOMZY010000286 DAOMZY010000000
VERSION     DAOMZY010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17633_length_4610_cov_4.479693"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PLK64_13745"
     CDS             complement(<1..652)
                     /locus_tag="PLK64_13745"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV80853.1"
                     /translation="MLGLDEAELAAYRMLVTGPSGTATEVSGALGVSVGKATRVIASL
                     EAKGLVARSGTAIDHVVASPPDIALGALIVEHQEGIRQAELEMRALRERYLAASSSRA
                     EQVVDVVRGREAVRQRFNQLQRGARREVLEFVKGDVVAVSVDENVEEEAAISRGVTYR
                     FLLERQTLERPGLMAAVSESIRDGVELRVTRELPIRLIVVDREIAMIPLAGATDDQS"
     gene            782..>1317
                     /locus_tag="PLK64_13750"
     CDS             782..>1317
                     /locus_tag="PLK64_13750"
                     /inference="COORDINATES: protein motif:HMM:NF017709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I9 family protein"
                     /protein_id="HPV80854.1"
                     /translation="MKRHQPPGKTLVTAALAISAFGVPLILSASPTQAAGAPPAPAAA
                     SALSQEQRTALTRLTGLSAPAIYVDAKTRASDDSVDVIVELRQGPAASEQARAAAAGR
                     VLSAATAQREAKEAQGRFKQFLTDTFAAKSVTYNIRKTYSDAYNGFALSISGKQLDRL
                     LESADVAAVWEDALVREAT"
     assembly_gap    1318..1417
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1418..>4610
                     /locus_tag="PLK64_13755"
     CDS             <1418..>4610
                     /locus_tag="PLK64_13755"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPV80855.1"
                     /translation="ESADVAAVWEDALVREATTPAMTTTPSNAAAAPAVVDASAEGVR
                     RLHAEGVTGKGLKVAILDTGIDYNHPALKDVYKGGYDFVNDDSDPMETTYADWKASGR
                     AEKDFNGNTYYTRHGSHVAGIIAGKPVAGADAMGIAPEADLYSYRVLGPYGGGTTSNI
                     LAGMDRALKDGANVVNMSLGANLNNPHSPLSAAADNLTLAGVTTVLAAGNDGQNGAFS
                     LGSPAASALSITVGANDTPVTVPTLKAAVGSGTADLRLLAQPFGDALTPLVGSTSDII
                     DVGRGLSADYSGKVVTGKVVFMRRGDSALENKITLAKTKGAKAVLLANNLADEGFIPY
                     YLGEGLNFLPTFTLTMAGGTALTDQLAAGATQVTLSDLGTTTFGGGILATFSSRGPAK
                     LTNDIKPEITAPGVSILSSVPPSSVDTAHLDDYTNAYQRLSGTSMATPYAAGVAALVL
                     QARPGLKPHEVKVAFMNTADALATEYGVFEMGAGQLDPYQAVHAATELVVQDRISIAD
                     GNSSHDWIPDLTGGLGFGLHATGEVVHDKRDVTVSNKTDQDITYSVRTVYSTSAGVAD
                     AAANGVRVSVPSDLRVKAKANMGLTVRLDVPATAAKGFYSGYVLIEGGNAAYRMPFGF
                     RVADKGIAALTALKPVMSTRTENVYSPQLSFAVNLKSHMRNLDAYIVDPASGQDIGYL
                     GALDPIGMREDVNYGPFAWTGKYFPITKDPRAPLGNRLTVLGEGHYQLRMVGTDDSDQ
                     QYSAITHFYVDNTPPKFTTALDGQRVVEVPNGATKYALNGSVIDGEVDKIKAAGIAIT
                     QADNLVLRFTFSTIQPSSAHAPQADGTFALTPSFGPSPSATDRYAAIDMAGNVGGRRE
                     FLVIKEGAPYIDSKSDKLALAPDQMATHSFTGQNADQWGEVKVTLRYSISNTELGEIR
                     KPAALAAYSTSPLVITKNSALDYPTVQITIPLDGPQLATGDNLPLLEVETIAKDGNWA
                     AATGFMTVSVTAKKRDGTSVNVNRLFDSVEMLNPTALLRGAFVAQGLLTEDSAIDNTI
                     DYSTIGAAASLTAPDGTVTQVPVSANRALSVDGV"
BASE COUNT          789 a         1708 c         1372 g          641 t
ORIGIN      
        1 cggactggtc gtcggtggcg ccggcgagcg ggatcatcgc gatctcgcgg tcgactacga
       61 tgaggcggat cggcaactcg cgggtcactc ggagctcgac tccgtcgcgg atcgactccg
      121 acacggccgc catcagaccg ggccgctcca gggtctggcg ctcaaggagg aatcggtagg
      181 tcaccccgcg actgatcgcg gcctcctcct cgacgttctc atcgacggac accgcaacca
      241 cgtcgccctt gacgaactcc aacacctccc gccgggcgcc gcgctgcaac tggttgaacc
      301 gttggcgcac cgcctcgcgg cctcgcacga cgtcgaccac ctgctcggcc cggctgctgg
      361 acgcggcgag gtaacgctcg cgcagcgccc gcatctccag ctccgcctgc cggatgccct
      421 cctggtgctc gacgatgagg gcgccgagcg cgatgtccgg cggcgacgcg acgacgtggt
      481 cgatggccgt tccggagcgc gcgacgagcc ccttggcctc caacgaggcg atcacccggg
      541 tggctttgcc caccgacaca ccgagggcgc cggacacctc cgtcgccgtt ccggagggcc
      601 cggtgacgag cattcggtat gccgcgagct cggcttcgtc tagtcccaac acactcagca
      661 agcctgcacc ctctttcggt gtctgaccgt ggcgagttcc cgccgtgtcg tattcacgat
      721 gttgtcacgc ctgtgggctg gtgtcactat caagacaccc cccactcagt tcggagccca
      781 catgaaacga caccagcccc ccggtaagac gcttgtcacc gctgcactgg cgatctcggc
      841 attcggcgtc cccctgatcc tgtcggcctc tcccacgcag gcagccggcg ctcccccggc
      901 ccccgcggca gcatcggccc tgagccaaga acaacgcacg gcgctcaccc gcctgaccgg
      961 cctttcggcc ccggccatct atgtcgacgc caagactcgg gcctccgacg actccgtcga
     1021 cgtcatcgtc gagttgcgcc aaggccccgc cgccagcgag caggctcggg ccgccgccgc
     1081 cggccgggtg ctctccgcag ccaccgccca gcgagaggcc aaggaggccc agggccgctt
     1141 caagcagttc ctcaccgaca cgttcgccgc caagtcggtg acctacaaca tccgcaagac
     1201 ctactccgac gcctacaacg gcttcgccct gtcgatctcg ggcaagcaac tcgatcggct
     1261 gcttgagtcc gccgacgtgg cagcggtctg ggaggacgcc ctcgtccgcg aggcgacnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttg agtccgccga cgtggcagcg
     1441 gtctgggagg acgccctcgt ccgcgaggcg acgacgccag ccatgacaac caccccgtcc
     1501 aacgcggccg cggctcccgc cgtcgttgac gcctcggccg agggcgtgcg ccgcctgcat
     1561 gccgaaggcg tgaccggcaa gggcctcaag gtcgccatcc tcgacaccgg catcgactac
     1621 aaccaccccg cgctcaagga cgtctacaag gggggctacg acttcgtcaa cgacgacagc
     1681 gaccccatgg agacgacgta tgccgactgg aaggcttccg gccgcgccga gaaggacttc
     1741 aacggcaaca cctactacac gcggcacggc tctcacgtcg ccggcatcat cgcgggcaag
     1801 ccggtcgccg gcgcagacgc gatggggatc gcccccgagg ccgacctcta ctcctaccgc
     1861 gtgctcggcc cctacggcgg tggcacgacc tcgaacatcc tcgcggggat ggaccgggcc
     1921 ctcaaggacg gcgcgaacgt cgtcaacatg tcgctcggcg ccaacctcaa caacccccac
     1981 agcccgctca gtgccgcggc tgacaacctc acgctcgccg gtgtgaccac ggtcctcgct
     2041 gcgggcaacg acggccagaa cggcgcgttc tccctcggct caccggcggc atcagcgctg
     2101 tccatcaccg tcggcgccaa cgacaccccc gtcaccgtcc cgaccctcaa ggcagcggtc
     2161 gggtccggca cggccgacct ccgactgctc gcgcagccct tcggcgacgc gctcacgccg
     2221 ctcgtcggga gcaccagcga catcatcgac gtcggccgcg gcctcagcgc cgactacagc
     2281 ggcaaggtcg tcacgggcaa ggtcgtgttc atgcgccgcg gcgacagcgc gctcgagaac
     2341 aagatcaccc tggccaagac caagggcgcc aaggccgtcc tgctcgccaa caacctcgcc
     2401 gacgagggct tcatccccta ctacctcggc gagggcctga acttcctgcc caccttcacc
     2461 ttgacgatgg ccggcgggac cgcactgacc gaccagctcg ccgccggcgc tacccaggtg
     2521 acgctcagcg acctcggcac gacgaccttc ggcggcggca tactcgccac cttcagttcg
     2581 cgcggcccgg ccaagctgac caacgacatc aagccggaga tcaccgcccc cggcgtgagc
     2641 atcctgtcct ctgtgccgcc gtcgtctgtc gacaccgcgc acctggacga ctacaccaac
     2701 gcctaccagc gactctcggg cacctccatg gccacgccgt atgccgcggg cgtcgccgcc
     2761 ctcgtgctgc aggcccggcc cgggctcaag ccccatgagg tcaaggtcgc cttcatgaac
     2821 accgcggacg cgctcgccac cgagtacggc gtcttcgaga tgggggccgg ccagctcgac
     2881 ccttaccagg ccgtgcacgc cgccaccgag ctcgtggtcc aggaccggat cagcatcgcc
     2941 gatggcaaca gcagccacga ctggatccct gacctcaccg gcgggctcgg cttcggcctg
     3001 cacgccacgg gcgaggtcgt tcacgacaag cgcgacgtca ccgtgtccaa caagaccgac
     3061 caggacatca cctactcggt gcgcaccgtc tacagcacgt ccgccggagt ggccgatgcc
     3121 gctgcgaacg gggtccgcgt gagcgtgccg tccgacctgc gggtcaaggc gaaggcgaac
     3181 atgggcctca ccgtccgtct cgacgtcccg gccaccgccg cgaaggggtt ctactcgggc
     3241 tacgtgctca tcgaaggcgg caacgcggca taccgtatgc cgttcggctt ccgggtcgcc
     3301 gacaagggga tcgccgcgct gacggcgctc aagcccgtga tgtcgacccg gacggagaac
     3361 gtctactccc cgcagttgtc cttcgcggtc aacctcaagt cgcacatgcg caacctcgac
     3421 gcctacatcg tcgacccggc gagcggtcag gacatcggct acctcggagc cctcgacccg
     3481 atcgggatgc gtgaggacgt caactacggg cccttcgcct ggaccggcaa gtacttcccg
     3541 atcacaaagg acccgcgggc gccgctcggc aaccgcctga ccgtgctcgg tgagggtcac
     3601 taccagctgc ggatggtcgg caccgatgac tccgaccagc agtactcggc gatcacccac
     3661 ttctacgtcg acaacactcc gcccaagttc accaccgcgc tcgacgggca gcgggtcgtg
     3721 gaggtgccca acggcgccac gaagtacgcc ctcaacggca gcgtcatcga cggtgaggtg
     3781 gacaagatca aggccgccgg catcgcgatc acccaggccg acaacctggt gctccggttc
     3841 accttctcga cgatccagcc gagctcggcc cacgccccac aagcggacgg cacctttgcg
     3901 ctgaccccga gcttcggccc gagcccgtcc gcgacggacc gctacgccgc cattgacatg
     3961 gccggcaacg tcggcgggcg gcgggagttc ctcgtcatca aggagggcgc gccctacatc
     4021 gacagcaaga gcgacaagtt ggcgctggca cctgaccaga tggcgaccca ctccttcacc
     4081 gggcagaacg ccgaccaatg gggtgaggtc aaggtgaccc tgcgctacag catcagcaac
     4141 accgagctgg gcgagatccg caagcccgca gcgctcgcgg catacagcac ctccccgctg
     4201 gtcatcacga agaactcggc tctggactac ccgacggtcc agatcaccat cccgctcgac
     4261 ggaccgcagc tggcgaccgg cgacaacctc cccctgctcg aagtggagac catcgccaag
     4321 gacggcaact gggctgcggc gaccgggttc atgacggtca gtgtcacggc gaagaagagg
     4381 gacggcacgt cggtcaacgt caaccgcctc ttcgactcgg tggagatgct caaccccacc
     4441 gcgttgttgc ggggcgcgtt cgtcgcccag gggctgctca ctgaggacag cgccatcgac
     4501 aacacgatcg actactcgac gatcggtgct gctgcgagcc tcactgcccc agacgggacg
     4561 gtgacccagg tgccggtttc ggccaacagg gcgctctcgg tcgacggggt
//
LOCUS       DAOMZY010000287         4557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_17874_length_4557_cov_7.755886, whole genome shotgun sequence.
ACCESSION   DAOMZY010000287 DAOMZY010000000
VERSION     DAOMZY010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17874_length_4557_cov_7.755886"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..983)
                     /locus_tag="PLK64_13760"
     CDS             complement(222..983)
                     /locus_tag="PLK64_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80856.1"
                     /translation="MRRFGAYIDETGDRGLGTNSSPIFGMAAIVIRETDLPSLREATA
                     QLRSDFGVPKDSTMSWKQHVKTHDRRKRAADVLSRVEGMTVCYVYADKSALTPGSYRD
                     NPTRFYNWVAFKTYKSVVWAARNMGANDLRIRFGHVRRHDHTTTHEYITREAAKDAKI
                     PDAIVTAMTWHGADRYTASQAADLCGGFLQAALWPHGAFGYTEPSYLLTMWPRIRVVN
                     GCAIPLGIMSMPNTDLLTRQPWFPCNGCPKTCDDA"
     gene            complement(1047..1949)
                     /locus_tag="PLK64_13765"
     CDS             complement(1047..1949)
                     /locus_tag="PLK64_13765"
                     /inference="COORDINATES: protein motif:HMM:NF025625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HPV80857.1"
                     /translation="MSGKQIKLFLVDGTPGGLTTAEITNWTGHVLTASRSDVAELLRR
                     DEASRTGVYLLLGEDSETSGGTQCYVGETDSIADRIKQHLRDKDWWDRVVVITSKDEN
                     LTKSHVRYLESRLISLAHQAARVTLSNGTAPPQTRLPEADRSDMDYFVSQLQIVLPVL
                     GVNAIRTRPAPAMPYGSPTADASPVFSLVNKRTGTDARAQVIDGEFTLLAGSRVVPAW
                     TATGTADSTRRSYAGYRAQHDHLVADGSIEVTDGVGVTTRDLVFGSPSTAGAVAIGRS
                     CNGRISWVTVDGIQFGAWEARGVE"
     gene            complement(2140..2730)
                     /locus_tag="PLK64_13770"
     CDS             complement(2140..2730)
                     /locus_tag="PLK64_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80858.1"
                     /translation="MPATEFSEFTYGYAVMQETEKFLTQHAGSLTMAPDQPSLITENI
                     VGYDAKFVTVDFAVLLQFKVPFYTSRQHSRGACGPGHCTWSAWGREHFRVGVEVGSNQ
                     QSAMRRYEREIDNGAYRGLSCYVTPAYWQRHEHNGHYLSGQVLDQSAAPLPSHFDGLS
                     GKHKFSYRAPSGPCVVMSEPREGPTSRCAHCSKARH"
     gene            complement(2732..>4557)
                     /locus_tag="PLK64_13775"
     CDS             complement(2732..>4557)
                     /locus_tag="PLK64_13775"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="damage-inducible protein"
                     /protein_id="HPV80859.1"
                     /translation="VGNPPYSVGQTSGNDNNANLRYPSLDARIEATYAARSTATLKNS
                     LYDSYIRAIRWASDRVGDAGVVGFVTNGGFVDSNTADGLRQSLIDEFSAVYVYNLRGN
                     QRTAGELSRREGGKVFGSGSRNTVAITFLVRTPGHSGPATLHYRDIGDYLTREDKLKL
                     VDADHLATVPWQQITPNAAGDWINQRGEEFDTFQPLGSKAPDSVFTLATSGLKSNRDA
                     WVYNFSKAVLTETVDRMVTFYNAEVERYESATAGGGPKPPVEGFINTDPTKFSWDRAD
                     RQRLPKGRTYTLRDGALRVSAHRPFARQAVWFDTELNNTVYQLPRLFPTAAHENYGVV
                     LTDAGSHFEFCAIATDLLPNLHLLDTGKFFARYTYEAVDSRAAQGALDFGTSDDVVIE
                     GYRRLDNITDETLTAYRAWYGDDVTKDGIFAFVYGLLHSPDYRSRFAADLKRSLPRIP
                     RVEAGGFAAFAEAGQQLLDLHIGYEDVEPWPLTVTGDQPPAGEDPYAWFRVEKLKWGG
                     SARDKDRSRIVYNPRVSITGIPDEAHEYLLGSRTAVEWVIDRYQVKTDKPSGIVNDPN
                     DYAREVGNPRYILDLIAKVTRVSVDTVEIVKALPPLRIVEG"
BASE COUNT          699 a         1451 c         1567 g          840 t
ORIGIN      
        1 cgacgggtta cctcgagctc aaggagttcc agcgcgtcga ggtcgtcgtc gcgccctacc
       61 agcgcagctg acctcgcgcg aggagtcaca cggggctgcg gcggtgcctg ctgagaggta
      121 gccttggcgt cgaagccctc aggaacctgc cgcggatcgc ccgcggtgct gccaccgctc
      181 tccttcagtg gatcggcccg cggaggttcc tgagggtttc gtcatgcgtc gtcgcacgtc
      241 ttggggcatc cgttgcatgg gaaccaaggc tgtctggtca gcaagtcggt gttcggcatg
      301 ctcatgatcc ccaacgggat tgcgcaccca ttgaccacgc ggatgcgcgg ccacatcgtc
      361 aacaggtatg acggttccgt gtagccgaag gcgccgtgtg gccagagcgc cgcctgcagg
      421 aaccctccgc agaggtccgc ggcttggctc gctgtgtatc gatctgcgcc gtgccatgtc
      481 atcgccgtca cgatggcgtc ggggatcttg gcgtccttcg cagcctcgcg ggtgatgtac
      541 tcgtgcgttg tggtgtggtc gtgtctgcgg acatgcccga acctgatcct caggtcgttc
      601 gcacccatgt tgcgagccgc ccagaccacc gacttgtagg tcttgaacgc cacccagttg
      661 tagaagcgcg tggggttgtc gcggtaggag cccggcgtca atgccgactt gtccgcatac
      721 acgtagcaga cggtcatgcc ctccactcgg ctgagcacgt cggctgcacg cttgcgccgg
      781 tcgtgcgtct tcacgtgctg cttccaggac atcgtcgagt ccttcgggac accgaagtcg
      841 ctgcgcaact gagcggtggc ttcgcgaagc gacggcaggt cagtctcacg aatcacgatg
      901 gccgccatcc cgaagatggg tgaggagttg gtgcccagcc cccggtcacc cgtctcatcg
      961 atgtacgccc cgaaccgcct cacacccgga ccgtacccat ggcatcccac cggcgcgcgc
     1021 tgagcgggat gccggtggac agttgcctat tcgacgccgc gggcctccca cgcgccgaac
     1081 tggatgccgt ccacggtgac ccaggagatg cggccgttgc aggagcggcc gatcgcgacc
     1141 gctcctgctg tcgacggcga cccgaacacg aggtctcgcg tcgtcacgcc gacgccgtcc
     1201 gtgacctcga tggagccgtc ggcaacaagg tggtcgtgct gggcgcgata gccggcgtag
     1261 gaccggcggg tgctgtcggc cgttccggtg gccgtccacg ccggcaccac gcgagagccg
     1321 gcgagcaggg tgaactcacc gtcgatcacc tgggctctcg catctgtgcc ggtgcgcttg
     1381 ttgacgaggg agaacaccgg ggaagcgtcg gcggtggggg agccgtaggg catcgccggt
     1441 gctgggcggg tgcggatggc gttgacaccg aggaccggca gcacgatctg cagctggctg
     1501 acgaagtagt ccatgtcgga gcggtcagcc tcgggcagcc gcgtctgcgg cggtgcggtg
     1561 ccgttgctca gggtgactct cgcggcctgg tgggccagcg agatgagccg tgactcgagg
     1621 tacctcacgt gcgacttggt gaggttctcg tccttggacg tgatgacgac gacccggtcc
     1681 caccagtcct tgtcgcgcag gtgctgcttg atcctgtcgg cgatcgagtc ggtctcgccg
     1741 acgtagcact gggtgccgcc cgacgtctcg gagtcctcac cgagcagcag gtagaccccg
     1801 gtgcgcgacg cctcgtcgcg acgcaggagt tcggcgacgt ccgatcggga tgccgtgagc
     1861 acgtgccccg tccagttcgt gatctccgcc gtcgtgagac cacccggtgt cccgtcgacg
     1921 aggaacagct tgatctgctt cccgctcact gcgcacccgc attgacgtgc cccatgagga
     1981 cgaacgcagc gccgagcaac gcggcggagt ccttgaggta ggcaacggca cctcgggccg
     2041 agcgcatctc gtacgcgccc cggtccagag agactgcccg aaccgcaggg ttctcggcga
     2101 caaaggcgtc aagccgtggc ctgcgcgagg tgcggtcatc tagtgtcgcg ccttggagca
     2161 atgcgcgcag cgacttgtgg gcccttcgcg tggctcggac atcacgacgc aggggccgct
     2221 gggcgctcga taactgaact tgtgtttgcc ggacagacca tcgaaatggg acggcagcgg
     2281 agctgccgac tggtcgagta cctgtccact gaggtagtgg ccgttgtgct cgtggcgttg
     2341 ccagtaggcg ggtgtcacgt agcacgacaa ccctcggtac gcgccgttgt cgatctcgcg
     2401 ctcgtacctg cgcatcgcgg attgctgatt cgagcccacc tcgaccccga cccggaagtg
     2461 ttcacgtccc caggccgacc aggtgcaatg acccggccca cacgcgccgc gcgagtgctg
     2521 gcggctggtg tagaacggca ccttgaactg cagcagcacg gcgaagtcga ccgtcacgaa
     2581 cttggcgtcg tacccgacga tgttctcggt gatgagtgac ggttggtccg gtgccatggt
     2641 caagctgccg gcatgctgcg tcaggaactt ctcggtctct tgcattactg cgtagccgta
     2701 ggtgaactcg ctgaactcgg tggccggcat gtcagccctc cacgattcgc aggggtggga
     2761 gggctttgac gatctcgacg gtgtcgacgc tgacgcgggt gaccttggcg atgaggtcga
     2821 ggatgtagcg cgggttgccg acctcgcggg cgtagtcgtt cgggtcgttg acgatgcccg
     2881 agggcttgtc ggtcttgacc tggtaacggt cgatcaccca ctccaccgcg gtgcgcgagc
     2941 ccagcaggta ctcgtgcgcc tcatcgggga tgccggtgat gctcaccctc gggttgtaga
     3001 cgatccgcga ccggtccttg tcgcgggcgc ttccgcccca cttgagcttc tccacccgga
     3061 accacgcata cgggtcctcg cctgcgggcg gctggtcgcc ggtcacggtc agcggccacg
     3121 gctcgacatc ctcgtagccg atgtgcaggt cgagcagctg ttggccggcc tcggcaaagg
     3181 cggcgaagcc acccgcctcg accctcggga tgcgcggtag cgagcgcttg aggtccgcgg
     3241 cgaaccgcga ccggtagtcg ggcgagtgca gtagcccgta gacgaacgcg aagatgccgt
     3301 ccttcgtcac gtcgtcgccg taccaggccc ggtatgccgt gagcgtctcg tcggtgatgt
     3361 tgtccagccg ccggtagccc tcgatgacga cgtcgtcgct ggtgccgaaa tcaagggccc
     3421 cctgcgccgc ccgggagtcc actgcctcgt aggtgtagcg cgcgaagaac ttgcccgtgt
     3481 cgagcaggtg caggttgggc agaaggtccg tggcgatggc gcagaactcg aagtgagacc
     3541 cggcatcggt gagcacgacg ccgtagttct cgtgcgccgc tgtcgggaac aggcggggca
     3601 gttggtacac ggtgttgttc aactcggtgt cgaaccagac cgcctgccgc gcgaatggtc
     3661 ggtgcgcgct gacccgcagg gcaccatcgc ggagtgtgta cgtacgtccc ttcggaaggc
     3721 gttgacgatc agcgcggtcc cacgagaact tcgtcgggtc ggtgttgatg aagccttcga
     3781 ccggaggctt cggcccaccg cctgctgtcg cactctcgta tcgctcgacc tcggcgttgt
     3841 agaaggtcac catgcggtcc acggtctcgg tgagtacggc cttgctgaag ttgtagaccc
     3901 aggcgtccct gttcgacttg agaccgcttg tcgccagcgt gaagacggag tcaggcgcct
     3961 ttgaacccag cggctggaag gtgtcgaact cctccccgcg ctggttgatc cagtcacccg
     4021 cggcgttcgg ggtgatctgc tgccagggca cggtggcgag gtggtcggcg tcgacgagct
     4081 tgagcttgtc ctcacgcgtc aggtagtcgc cgatgtctcg gtagtgcagg gtcgctggcc
     4141 cgctgtggcc cggagtgcgc acgaggaagg tgatcgccac ggtgttgcgg gacccgctgc
     4201 cgaacacctt gccgccctcc cggcgagaga gctccccggc ggtgcgctgg ttgccacgca
     4261 ggttgtagac gtacacggcc gagaactcgt cgatcaggct ctggcgcagt ccgtcggcgg
     4321 tgttggagtc gacgaagccg ccgttggtca cgaacccgac gaccccggca tccccgaccc
     4381 ggtcactcgc ccagcggatc gcgcggatgt aggagtcgta gaggctgttc ttcaacgtcg
     4441 cagttgagcg ggcggcatac gtcgcctcga tccgggcgtc caggctcgga tagcgcaggt
     4501 tcgcgttgtt gtcgttgccg gaggtctgcc ccaccgagta cggcgggttg ccgacga
//
LOCUS       DAOMZY010000288         4514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_18105_length_4514_cov_6.366226, whole genome shotgun sequence.
ACCESSION   DAOMZY010000288 DAOMZY010000000
VERSION     DAOMZY010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18105_length_4514_cov_6.366226"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="PLK64_13780"
     CDS             <1..917
                     /locus_tag="PLK64_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HPV80860.1"
                     /translation="DVEAGGAMLWQHLFWFFGHPEVYIIALPFFGIVSEVVPAFSRKP
                     LFGYKTMVGSTVAIMALSMAVWAHHMYATGQVLLPFFAIMSMLIAVPTGVKFFNWIGT
                     MWRGSLTLSTPMIWSIGFIVTFLFGGMTGVLLASPALDFHVTDTYFIVAHFHYTVFGT
                     VVFAMFSGFYFWWPKLTGRMLDERLGKIHFWMLFIGFHTTFLVQHLLGMQGMARRYAD
                     YLPEEGFALGNQISSVGAFLLGASTLPFLYNVWKTWRTAPLVEVDDPWGYGGGLEWAT
                     SCPPPRHNFTSLPRIRSERPAFDLHHPT"
     gene            complement(1374..3299)
                     /locus_tag="PLK64_13785"
     CDS             complement(1374..3299)
                     /locus_tag="PLK64_13785"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPV80861.1"
                     /translation="MATQCTANVFITNQTDGTAQIQLSHMNNSNGNQAGAWTAAPGQT
                     VGPLVVHFVTGFGTGGILDWWTVAMSVENGSTPGIYQNTGTLAFPHWKECQLQSGDVN
                     KDLHFTVSTTDFSTNLASGGCHDGMTRLSNYSPIRNVFVLMLENRSFDHMWGRSGIPG
                     LTVPAPGTSNSYNGTSYPVSSPAPTAMTGDPGHEFADVLEQLAGAGATYPPGGPYPPI
                     TMSGFASNYATTTDEALPPPTAAHIGDVMMEFDTPTQMPTSYALATSFAICDHWFCSI
                     PGPTWPNRFFVHGASSAGLEHAPTTAQIVEWETVAGFVLPHGSIYDALTANKLQWRLY
                     NDNTDAYSDNPQNGSLFGALPQVTALKNVTLLEVNSLTHFASDLQGPYPYQYTFIEPN
                     YGDVTSNTYVGGSSQHPMDDIYGGEALIKGVYEAIRNSPLWETSLLIITYDEHGGFYD
                     SVAPPPAAPPADGSGTAYNDTGFAFDLLGVRVPAIVVSPLIAAGTVDHTVYDHTSALA
                     TVERLFGFGALTARDAAANDLRHLFTQTTARTDCPTTLPAPVARMGRVLPDGASEAAA
                     AAEDDLPLEDSGNLPAFLATARKAAYDTYATTPQERAIVDEEYAAITTRGEAKAFVAR
                     VMAKATLVRDSQPRPVM"
     gene            3552..>4514
                     /locus_tag="PLK64_13790"
     CDS             3552..>4514
                     /locus_tag="PLK64_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPV80862.1"
                     /translation="MSTVPRLTVPASADADEVYAAYLAWAEAQGLTLYPHQDEAIIEV
                     LSGAHVVLATPTGSGKTLVATAAHLAALAADRVSFYTAPIKALVNEKFFALCDIFGAD
                     QVGLLTGDASVNPDAPVICCTAEVLANIALREGAAADIGLVVMDEFHYYGDPQRGWAW
                     QVPLLELTDVQFVLMSATLGDMSKIVADLERRTGRPVAQVTTAERPVPLTYTWSLTPL
                     AEVVEEIVTTRRAPVYVVHPSQAAAAEHAQSLRGLTLLTHAERDEIADRIGPVRFASG
                     FGKVLSALLRKGIGVHHAGMLPRYRRIVEQLAQAGLLKVICGTDT"
BASE COUNT          679 a         1423 c         1583 g          829 t
ORIGIN      
        1 tcgatgtcga ggccggcggg gcgatgctct ggcaacacct gttctggttc ttcgggcacc
       61 cggaggtcta catcatcgcc ctgccgttct tcggcatcgt cagcgaggtc gtgccggcgt
      121 tcagtcgcaa gccgctgttc ggttacaaga cgatggtggg gtcgacggtc gcgatcatgg
      181 ccctgtcaat ggccgtgtgg gcgcaccaca tgtacgccac gggccaggtg ctgttgccgt
      241 tcttcgcgat catgtcgatg ctcatcgcgg tgccgacggg ggtgaagttc ttcaactgga
      301 tcggcacgat gtggcgaggt tcactcaccc tgtccacacc catgatctgg tcgatcgggt
      361 tcatcgtgac gttcctcttc ggcggcatga ccggggtgct gctggccagc ccggcgttgg
      421 acttccacgt caccgacacc tacttcatcg tggcccactt ccactacacg gtgttcggca
      481 cggtggtgtt cgcgatgttc tctggcttct acttctggtg gcccaagctc accgggcgga
      541 tgctcgacga gcgactcggg aagatccact tctggatgct cttcatcggg ttccacacga
      601 cgttcctcgt ccagcacctg ttggggatgc agggcatggc gcgccgctat gccgattacc
      661 tccctgagga ggggttcgcc ctcggcaacc agatctcctc ggttggtgcg ttcctgctcg
      721 gtgcttcgac gctgccgttc ctctacaacg tctggaagac ctggcggacc gcgcccctgg
      781 tcgaggtcga tgatccttgg ggatatggcg gagggctcga gtgggcgacg tcttgtccgc
      841 cgccgcgcca caacttcacc agcctgccgc ggatccgctc cgagcgcccc gccttcgacc
      901 tccaccaccc cacctgaccg aggacagcca cggagatagc gctagcacgg tcatttgtgg
      961 gggtccaagg acggtttggg gcccggagat agcgctcgca cggtcatctt tgccgggtcg
     1021 agggtggcgg gctggagata gcgctcgcat ggtcatttgt gggggcccag gatgggttga
     1081 ccgccggtga tagcgctcgc atggtcattt gtgggggccc aggatgggtt gaccgccggt
     1141 gatagcgctc gccggtcatc tttgccgggc gaggcaggct catggggggc cggggcactc
     1201 acatcggggc ctgggctagg cgcgggcgtg acggagtgcg tttgaggggg tgatcacccc
     1261 gccaaacgca ctccgtcgag cacgctgccg cggcgctgct cgaaccaacc ggcagcgggc
     1321 ccacaacagc agtgggccga ccaacggcag cgggccgacc aggggcagcg gacctacatc
     1381 accgggcgcg gctgtgagtc gcggacgagg gtcgccttgg ccatcaccct ggcgacgaac
     1441 gccttcgcct ccccacgtgt ggtgatcgcg gcatactcct cgtcgacgat cgcccgctcc
     1501 tggggcgtgg tcgcataggt gtcgtatgcc gccttgcgag ccgtcgcgag gaaggccggc
     1561 aggttgccgc tgtcctccag aggcaggtcg tcctctgccg cggccgccgc ctcgctcgcg
     1621 ccgtcgggca ggacccgacc catccgggcg accggcgccg gcagcgtcgt cgggcagtcg
     1681 gtccgtgccg tggtctgcgt gaacaggtgc cgcaggtcgt tggccgccgc gtcgcgggca
     1741 gtcagcgcgc cgaagccgaa cagtcgctcc accgtggcga gcgccgaggt gtggtcatag
     1801 accgtgtggt cgaccgtccc cgcggcgatg aggggtgaga cgacgatggc cggcacgcgc
     1861 acgcccagca ggtcgaaggc aaagcccgtg tcgttgtatg ccgtcccgct cccgtccgcg
     1921 ggcggggcag ccggcggcgg cgcgaccgag tcatagaagc cgccatgctc gtcgtaagta
     1981 atgatgagca ggctcgtctc ccagagcggg gagttgcgga tcgcctcgta cacgcccttg
     2041 atgagcgcct cgccgccgta gatgtcatcc atcgggtgct gcgaggaccc gccgacgtag
     2101 gtattgctgg tgacatcgcc gtagttgggc tcgatgaacg tgtattggta gggatagggg
     2161 ccctgcaggt cgctggcgaa gtgggtcagc gagttgactt ccaggagggt cacgttcttc
     2221 agcgcagtca cctggggaag ggcgccgaac agcgaaccgt tctgagggtt gtcgctgtag
     2281 gcgtccgtgt tgtcgttgta gaggcgccac tggagtttgt tggccgtgag cgcgtcgtag
     2341 atgctcccgt ggggcagcac gaaaccggca actgtctccc actcgacgat ctgtgcggtc
     2401 gtcggagcgt gctccaggcc ggccgacgat gccccgtgga cgaagaaccg gttgggccag
     2461 gtcggccccg ggatcgaaca gaaccagtgg tcacagatgg cgaatgaagt ggccaaggcg
     2521 taggacgtcg gcatctgggt gggggtgtcg aattccatca tgacgtcgcc gatgtgggcg
     2581 gccgtcggcg gcgggagagc ctcgtccgtc gtggtggcgt agttgctcgc gaacccactc
     2641 atcgtgatcg gcggatacgg cccgcctggg gggtaggtcg cgccagcgcc ggccaattgc
     2701 tccaggacgt cagcgaattc gtggccagga tcgccagtca tcgccgtcgg ggccggtgag
     2761 ctcacggggt aggacgtccc gttgtaggag ttgctggttc ctggggcggg gaccgtcaga
     2821 cccggtatgc cggaccggcc ccacatgtgg tcgaaggacc ggttctcgag catgaggaca
     2881 aagacgttgc ggatggggga gtagttcgac aaccgggtca tgccgtcatg gcagccgccg
     2941 gacgccagat tcgtggagaa gtcggtcgtg ctgacggtga agtggaggtc cttgttgacg
     3001 tcgccgctct ggagttggca ttccttccag tgcgggaagg ccagcgttcc ggtgttctgg
     3061 tagatcccgg gagtcgagcc gttctcgacg ctcatcgcca ccgtccacca gtccagaatg
     3121 cccccagtgc cgaagccggt cacgaagtgg acgacgagcg ggccgacagt ctgtcccggc
     3181 gctgccgtcc atgccccggc ttggttgccg ttggagttgt tcatgtggga gagctggatc
     3241 tgggcggtgc cgtcggtctg attcgtgatg aagacgtttg cagtgcactg tgtcgccatg
     3301 gtggaactcc tcatcgggtc ggccaatcgc cccgagcgcg gctgaacggt gctgggggag
     3361 ggggagagca ctcgggcccg agtgatgaag cgctagctcc cccagttgat cacggttcgt
     3421 aagtgagtgg acccttacca ctcgaatttc ctgacgttcc cgccgaattc gtcccgcgtg
     3481 cccaaggctg ggcgttgcgg gcgggcgcgg acggcatact tggtgggcgt gggcctgcga
     3541 ggatgggtgg gatgtccacc gtcccgcgcc tcaccgtccc cgcgtccgcc gacgccgacg
     3601 aggtgtatgc cgcgtacctc gcctgggcgg aggcccaggg gctcaccctc tatccccacc
     3661 aggacgaggc gatcatcgag gtgctgtccg gcgcacacgt cgtgctggca acgcccaccg
     3721 gctccgggaa gacgctcgtc gccacggccg cccatctcgc ggctctcgcg gccgaccggg
     3781 tgagtttcta caccgcgccg atcaaggcgc tggtcaacga gaagttcttt gccctgtgcg
     3841 acatcttcgg cgccgaccag gtggggctgc tgaccggtga cgcctcggtc aaccccgacg
     3901 cgcccgtgat ctgttgcacc gcagaggtgt tggccaacat cgccctgcgt gagggagcgg
     3961 cggcggacat cgggctggtc gtcatggacg agttccacta ctacggcgac ccgcagcggg
     4021 gctgggcctg gcaggtgccg ctgctggagt tgaccgacgt ccaattcgtg ctgatgtcag
     4081 ccacattggg cgacatgagc aagatcgtcg cagacctcga gcgacgcacc ggccggccgg
     4141 tggcgcaggt gacgactgcc gaacgcccgg tgccgctgac ctacacgtgg tcactgaccc
     4201 cactcgcgga ggtcgtcgag gagatcgtca ccacgcgccg ggcccccgtc tacgtcgtcc
     4261 atccgtcgca ggccgccgcc gccgagcacg cccagtcgct ccgtggcctg accctgctga
     4321 cccacgccga gcgcgacgag atcgccgacc ggatcgggcc ggtgcgtttc gcgtccgggt
     4381 tcggcaaggt gctgtcagcc ttgctgcgca agggaattgg cgttcaccac gcggggatgc
     4441 tgccgcgcta tcggcggatc gtcgagcaac tcgcgcaggc cggcctgctc aaggtcatct
     4501 gcgggaccga cacc
//
LOCUS       DAOMZY010000289         4494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_18203_length_4494_cov_4.127506, whole genome shotgun sequence.
ACCESSION   DAOMZY010000289 DAOMZY010000000
VERSION     DAOMZY010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18203_length_4494_cov_4.127506"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1004
                     /locus_tag="PLK64_13795"
     CDS             81..1004
                     /locus_tag="PLK64_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPV80863.1"
                     /translation="MTLTLVLIAGAICGVGILALVLLLDVRPARGPAALARLDLDRRR
                     FRREAVLEVDALHAYESMRLRRVGAALRESLEARGIRLPAGLQADLAITGRSVENHLA
                     ISLASGLAGLMLPLLLVAPFAAFFGLGMGLVPLWIALLLGALGAILPSLSVQSRAADL
                     RRDFRHVVSSFLDLVAMNLSGGRGVPEALEVASSVSTYWGMSRIREKLRNARLQGVTP
                     WSALGELGQELAIDELRDLASALTLVAEDGAKVRDSLFARASSMRQRELADAEGRAAA
                     RSQSMVVAQMLLAIGFLTFLIFPAVARLLSS"
     gene            1156..1374
                     /locus_tag="PLK64_13800"
     CDS             1156..1374
                     /locus_tag="PLK64_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80864.1"
                     /translation="MYVKSVGAAHYLEAALRARLGHARADRGASVIEWVIISAVLVIL
                     VGVVGGILYTKINSAANAVNLAPAVGGP"
     gene            1337..1789
                     /locus_tag="PLK64_13805"
     CDS             1337..1789
                     /locus_tag="PLK64_13805"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HPV80865.1"
                     /translation="MRSTSLRPSVAPDRRRGDAQPHGGRRERGSSVIELTLYMPILML
                     VVFVAVQFSLTYLGNQVVSSVARETARAVRQGADTGQANAVAVDYAQKIGSGVISNLS
                     VTVTHPDADTVRVRVAADTLHLVSISSLSRVSAEVQGPMETFRPDTGP"
     gene            1791..2198
                     /locus_tag="PLK64_13810"
     CDS             1791..2198
                     /locus_tag="PLK64_13810"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HPV80866.1"
                     /translation="MRARRGRERGSMAIEVIILVPILFSIIMLIVAGGRLVQRQGQVD
                     SAARDAVRAASMERSIDAARTAASQTANVTLGGQPCQTADLSGSDFRAGGLVRVRLTC
                     TVSYADLGLVGMPGQTTVTSVSTAPLDIFRRAE"
     gene            2195..2680
                     /locus_tag="PLK64_13815"
     CDS             2195..2680
                     /locus_tag="PLK64_13815"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HPV80867.1"
                     /translation="MTRAARRQPAFSSQLRLRRRRATERGASTIFVIGMVAVLFAVAG
                     LAIDGGRVLNGKDRAYDVAEQAARAGANQLNIAAVRSAGGGQVLLDETAARNAAAAFV
                     ASVPGYSVEGIATSPTQVTVRVRGTVNSVLLSLAGFSTFTVRGDATASPVTGITSGAI
                     P"
     gene            2677..>4494
                     /locus_tag="PLK64_13820"
     CDS             2677..>4494
                     /locus_tag="PLK64_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80868.1"
                     /translation="MSGQSERGLLERTTTTTPPSGPERFRPAPAPAQRAPLGVRLLSA
                     LGLLALVIGVPALLFWLSGPPPIPTRLPTREDIAGQIGIEQVVTILVAIVWLAWLQFV
                     ACVIVEAFAAVRDGGIPKALPFSGPSQRLARVLVGALLLSGVVAGQVSAVVSAVSAEN
                     LRATSSISQSVATSSAVAPTTEAPATATAATQLPSTAVGAHPDGPKVYTVAPPVGRHH
                     DSLWDIAERHLGDGRRYNEIFALNEGRAQPDGRALHLARLIQPGWQLVMPEDAIGVER
                     MHLESAPVPSSAASESAATRAVPGTERAEAGTAGDLAQGAGSVGAVSTAAASTAAAST
                     SAASSASSVDQGGTGGILDVSSPAAPVNPLVAAFAMSGTFAACLLAAVLRQRRRSGGG
                     GQAGDDALDVEVGLRAGADPRRLAFVDGALRALATDCSATGLPVPQIYAARADDETFT
                     VLLSPSRADAPDRWSVHDDGARWEMQRSMLGRGAATGPAAYPALVSLGRDRQGRDVFV
                     DLEAAGGPIQVSGDGVVVGQVITALAVQLATVPWTDGVTVSAFGLPKHVRAIAADRIR
                     VVDNPQDVVRRFGERSGVQGQGVLTGRVVRPVDVLPEYLL"
BASE COUNT          581 a         1611 c         1559 g          743 t
ORIGIN      
        1 ctgcgccggt tggcgacctt ccagatgccc gcccgcttcc tgcaggctcc gcccaccgac
       61 gtgacgtcgg gagccccccg atgaccctga cactcgtgct catcgccgga gccatctgcg
      121 gcgtcggcat cctcgcgctc gtgctcctct tggatgtccg accggcccgg ggaccggctg
      181 ccctggcgcg gctcgatctg gaccggcgcc ggttccggcg ggaggccgtc ctcgaggtcg
      241 acgccctgca cgcctacgaa tcgatgcggc tacggcgcgt cggtgcggcc ctgcgcgaga
      301 gcctcgaagc ccgtggcatc cggttgccgg ccggactgca ggccgacctg gccatcaccg
      361 gacgttcggt cgagaaccac ctggcgatct cgctggcctc ggggctcgcc ggactgatgc
      421 tgcccctgct gctcgtcgcc ccgttcgcgg ccttcttcgg gcttggaatg ggcctggtgc
      481 cgctgtggat cgccctgctc ctcggagccc tcggcgcgat cctgcccagc ctgagcgtgc
      541 agtcccgggc agccgacctg cggcgggact tccggcacgt cgtcagcagc tttttggatc
      601 tggtcgcgat gaacctcagc ggcgggcgcg gagtccctga ggccctcgag gtcgcgagtt
      661 cggtgtccac ctattggggg atgtcccgga tccgggagaa gctgcgtaac gcgcggctcc
      721 agggcgtcac cccgtggtcg gccctgggtg agctcggcca ggagttggcg atcgacgaac
      781 tgcgtgatct ggcgtccgcg ctgaccctcg tggccgagga cggcgccaag gtgcgtgact
      841 cgctgttcgc ccgcgcgtcc tcgatgcgcc agcgcgagtt ggccgatgcc gaggggcgcg
      901 ccgccgcccg ctcgcaatcg atggtggtgg cgcagatgct gttggcgatc ggcttcctca
      961 ccttcctcat ctttcccgcc gtcgcacgac tgctcagcag ttgagtgccg ggggaggacg
     1021 ggctcaccga gcccgtcaag tgccgccagg gcgccagcgc tcgaagcggt ttcgcaaggt
     1081 cacactgatt cggggtaacc tgaggcgttc ggtcacgccg caggtcattc tcggcaactg
     1141 ctgaaagggg acggcatgta cgtcaagagt gtcggagctg cgcactatct ggaggccgcc
     1201 ctgcgtgccc ggctggggca cgctcgggcc gaccggggtg cctcggtcat cgagtgggtg
     1261 atcatcagcg ctgtgttggt gatcctcgtc ggggtcgtcg gaggcatcct ctatacgaag
     1321 atcaactcgg cagcgaatgc ggtcaacctc gctccggccg tcggtggccc ctgaccgtcg
     1381 gcgcggggac gcgcagccac acggggggcg tcgcgagcga gggtccagcg tcatcgagct
     1441 gaccctctat atgcccatcc tcatgctggt cgtcttcgtt gccgtccagt tctcgctgac
     1501 ttacctgggc aaccaagtgg tgtcgtcggt ggctcgagag accgcgcgcg cggtgcgaca
     1561 aggtgccgac accggtcagg cgaacgcggt cgcggtggac tacgcccaga agatcggttc
     1621 cggggtgatc tccaacctgt ccgtaacggt cacgcacccc gacgccgaca cggtccgtgt
     1681 ccgggtcgcc gctgacacgc tgcacctggt gagcatctct tcgctgagcc gggtgagcgc
     1741 ggaggtccag gggccgatgg agaccttccg cccggacaca gggccctgac gtgcgcgcgc
     1801 gtcgtggtcg ggagcgcggg tcgatggcca tcgaggtcat cattctcgtg ccgatcctct
     1861 tctcaataat catgctcatc gtcgcgggcg ggcgcctcgt ccaacgccag ggccaggtgg
     1921 attccgcggc gcgagatgcc gttcgggcgg cgtcgatgga gcggtctatc gacgcggcga
     1981 ggacggcagc cagccagacg gccaacgtca ccctgggcgg ccaaccgtgc cagacggccg
     2041 acctctcggg ctcggacttt cgggccggtg ggctcgtgcg ggtgcgtctg acctgcaccg
     2101 tctcctacgc cgatctcggc ctggtcggaa tgccgggcca gacgaccgtg acctcggtga
     2161 gcaccgcccc cctcgacatc ttccggaggg ccgagtgacc cgcgcagccc ggcgacaacc
     2221 ggccttctca tctcaactgc ggctgcgccg tcgacgtgcc accgagcgcg gcgcctcgac
     2281 gatcttcgtc atcggcatgg ttgccgtcct cttcgccgtg gcggggttgg cgatcgatgg
     2341 tggccgcgtg ctcaacggca aggaccgggc ctacgacgtc gccgagcagg ctgctcgcgc
     2401 cggggccaac cagctcaaca tcgccgcagt gcgctccgcg ggcggcggcc aagtgctcct
     2461 cgacgaaacc gctgctcgca acgccgcagc cgccttcgtg gcgtccgtcc ccggctattc
     2521 ggtcgaaggg atcgcgacct cgccgaccca ggtgaccgtg cgcgtccgag ggacggtcaa
     2581 cagcgtgctg ttgagcctgg ctgggttcag caccttcacc gttcggggag acgcaaccgc
     2641 gtcacccgtc accggcatca cctcgggagc tattccatga gtggacagag cgaacgcggc
     2701 ctgttggagc ggaccacgac caccacgcca ccgagcggac cggagcggtt ccgcccggct
     2761 cccgcccccg cgcagcgggc cccgctgggc gtgcggttgc tgagcgcgct tggcctgctc
     2821 gcattggtca tcggggtccc ggccctgctg ttctggctgt ccggaccgcc gccgatcccc
     2881 acgcggttgc ccacccgtga ggacatcgcg ggtcagatcg gcatcgagca ggtcgtgacg
     2941 atcctcgtcg ccatcgtctg gctcgcgtgg ttgcagttcg tcgcctgtgt catcgtcgag
     3001 gccttcgccg cggtgcgcga tggcggcata cccaaggcgt tgcccttctc cgggcccagc
     3061 cagcggttgg cgcgagtctt ggtcggggct ctgctgctgt cgggagtcgt cgccggacag
     3121 gtgtccgccg tcgtctcggc cgtctctgcg gagaacctcc gcgcgacctc gtcgatctcg
     3181 cagtcggtcg cgacctcaag cgccgttgcc cccacgaccg aagccccggc gacagcaacc
     3241 gcggccaccc agctgccctc gacagcggtc ggtgcccacc ccgacggccc gaaggtctac
     3301 accgtcgccc caccggtcgg tcgccatcac gactccctgt gggacatcgc cgaacgccat
     3361 ctgggtgacg gtcgccgcta caacgagatc ttcgccctca acgagggccg cgcgcagccc
     3421 gacggtcggg ccctgcacct ggcccggctg atccagccgg gctggcaact ggtgatgccc
     3481 gaggatgcga tcggcgtgga gcgcatgcac ctggagtcgg ctcctgtgcc atcgtccgct
     3541 gcgagcgagt ctgcggccac gcgtgccgtc cccggcaccg aacgggccga ggccggcacc
     3601 gccggagacc tcgcccaagg ggccggctcc gtcggcgctg tcagcaccgc agcagccagc
     3661 accgcggccg cgagcacctc ggccgccagt agcgcgtcct cggtcgacca gggtggcacc
     3721 ggcggcatcc tcgacgtgag cagcccggct gccccggtca acccgctggt ggcggcgttc
     3781 gccatgagcg gcaccttcgc cgcgtgcctg ctggccgcgg tcctgcgcca acgccgccgc
     3841 agcggaggtg gcgggcaggc cggtgacgac gctctcgacg tcgaggtcgg cctgcgcgcc
     3901 ggcgccgacc cccgtcgcct ggccttcgtc gacggcgccc tgcgggcctt ggccaccgac
     3961 tgctccgcca ccgggctgcc cgtgccccag atctacgctg ctcgcgccga tgacgagacc
     4021 ttcaccgtgc tgctgtcccc ttcccgcgcc gatgcgcccg accggtggtc ggtgcacgac
     4081 gacggcgcgc gttgggagat gcagcgctcg atgctcggtc gaggagcggc aaccggcccc
     4141 gcggcatacc cggcgctggt gtccttgggc cgcgaccgtc aggggcgcga cgtgttcgtc
     4201 gatctcgagg cagccggagg gcccatccag gtcagcggtg acggggtggt cgtcggtcag
     4261 gtcatcacgg ccctggccgt ccagctggcc acggtgccgt ggacggacgg cgtcacggtg
     4321 agcgccttcg ggttgcccaa gcacgttcgg gccatcgctg cggaccggat tcgcgtcgtc
     4381 gacaacccgc aggacgtcgt tcgtcggttc ggcgagcgct ccggcgtcca gggccagggc
     4441 gtgctcaccg gccgggtcgt tcggcccgtg gacgtgctgc cggagtatct cttg
//
LOCUS       DAOMZY010000290         4465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_18366_length_4465_cov_6.264626, whole genome shotgun sequence.
ACCESSION   DAOMZY010000290 DAOMZY010000000
VERSION     DAOMZY010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18366_length_4465_cov_6.264626"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1932
                     /gene="murJ"
                     /locus_tag="PLK64_13825"
     CDS             172..1932
                     /gene="murJ"
                     /locus_tag="PLK64_13825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HPV80869.1"
                     /translation="MPNAEEPQSGSSGADPQTAPPVETRRSRRESGRGNGRGAPGSGG
                     SVARNSASMALGTLGSRVLGFIRTAMLTAVIGGALAADSFTVANSLPTQVYVLINGGL
                     LSALIVPQITKAMARQDGSRDFTDRLLTLAFLVLAGVTAICLPAAPWIIDLLSANKSP
                     DFLSLTTALAYICMPQVFFYGLYSVLGQVLNAYGRFSAYAWAPAWANVIQIAGLAYFI
                     REWGQQPTLTTWTPTMVWVLAGTTTLGIVVQGLCLIVPLMRSGFRYRPRFGWRGYGFG
                     DLSRMAGWTIAALTVSQLGGLVTTRAMTVGASQAPNVAGNGIQQYAYSLYTLPHSLIT
                     VSIVTALFPAMSRAFQEGDLAGLRRWVVTGLTAPAVLVIPAATVLIALGRPMVSALFP
                     GLRVVPALGVDEPGNVALVLALMSIGILPFGITALKQRYCFARGDGWLNFWQVGVMTL
                     GNLITAVVAMVATPPEYVVAVVAAGGTISNIVGAGLFLLVARRQLDGLALRGVWFLWL
                     RLTVASGVAGLAAWAVASYIAAPTSRWLLQAIALIVGGVLFVLAFVVASRLLRIGEVT
                     EVLTRLTSRITGRLGARSAR"
     gene            complement(1942..2682)
                     /locus_tag="PLK64_13830"
     CDS             complement(1942..2682)
                     /locus_tag="PLK64_13830"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016664279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HPV80870.1"
                     /translation="MSAEGWSSPIVGVMGGLGPAATVSFLDLLVRLTKADRDQDHIDA
                     IVLQHATTPDRTAALLDPTAPDPTPALVADAQRLERMGADFLVLPCNSANIFTEPVER
                     AISIELLSIVQETASVALALADGPIAVFATEGTIHAGTYHEAITRGGGIAWTPPRPLQ
                     ATISAIIYDQVKAGRPVDLGALHASIDVAREAGCSHVIFGCTELSVVYDREGLSERVD
                     VVDSLRTLAEATILKAGRALVERGTAPA"
     gene            complement(2679..3959)
                     /locus_tag="PLK64_13835"
     CDS             complement(2679..3959)
                     /locus_tag="PLK64_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80871.1"
                     /translation="MFTRIAEPGGGSAPEFVPVILGGHVGAYSLARAFHEGYGVRSLV
                     VTGGDTWVTDHSTIMEKVHCPDLLDEDTLVRTLTGAPFTDITVPMLLLATSDTLVEKV
                     VAVADRVGPGFRVPYVGVEVLERATRKHTFDEVAQKAGVSTPATAIIDLGGDDWHEVQ
                     APGPFPLILKATAPTAWHGVSFEGQAKVHEVASEAELQELVARIHAAGYRDKLVLQDR
                     IPGDDTGMRILTCYCDRDSNVRFASWGQTLIEEHSPGQLGVPAAILTGVNSEVVAQAT
                     RLLAELRWVGYANFDLKYDPRDGSVKFFELNPRLGRSNYYVTGTGHNAVSYYVDEYIR
                     DTLGPVGPVLESTAETIYSVVPVRTIKWALQGHPELWEKVHEVIQAGGLRNPLHYRAE
                     KDPRRWVKVALGGLSYDRKFHRYYDPLGEIGSGT"
     gene            complement(3940..>4465)
                     /locus_tag="PLK64_13840"
     CDS             complement(3940..>4465)
                     /locus_tag="PLK64_13840"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HPV80872.1"
                     /translation="NITISLVRLDPALARAAAQKLAAESTSQLGKLRSKPHSEKQDRQ
                     IETLEQSVANCAALVAEMDAIEAVTPRGPYLAGSVFATCGSRGSYLYAASSNDYRNLR
                     PNYLMVWELMKRASAAGVATFDFGGIDASEGLEEFKRQWNPQRLEYAGEFTLRLRPLA
                     GRLVEALVRVYKNR"
BASE COUNT          642 a         1532 c         1540 g          751 t
ORIGIN      
        1 gcttgggcgt cggcatgggc ccgaagggat cacgaagtac accgagagcc agaccatcgc
       61 cgctcagcac ctcgtgccga tcgcgccgtt cggcggtctg gacgatcggc agttcgccac
      121 agcgttgacc gtgggcctca aactcctgcg gcgcgccggg ctgcggtgac cgtgccgaac
      181 gccgaggaac cgcagtcggg gtcctcgggc gctgatccac agacagcgcc accggtcgaa
      241 acccgacgca gtcggcgcga gagtggccgt gggaacggtc gcggtgcccc cggcagcggt
      301 gggtcggtcg cccggaacag cgcctcgatg gcgctcggca cgctcggctc tcgggtcctg
      361 gggttcattc gcacggcgat gctcactgcg gtgatcggcg gcgccctcgc cgcagactcg
      421 ttcaccgtcg cgaactcgct gccgacgcag gtctacgtcc tcatcaacgg cggtctgctc
      481 tcggcgctca tcgtcccgca gatcaccaag gcgatggccc ggcaggacgg gtcgcgggac
      541 ttcaccgacc ggctgctcac cttggcgttc cttgtcctgg ccggggtcac cgccatctgc
      601 ctgccggcgg cgccctggat catcgacctg ctcagtgcca acaaaagccc ggacttctta
      661 tcgctgacga cggcgctcgc ctatatctgc atgccccagg tgttcttcta cgggctctat
      721 tcggtgctcg ggcaggtcct caacgcctac gggcggttct cggcgtatgc gtgggcgccg
      781 gcctgggcca acgtcatcca gatcgccggg ctcgcctatt tcatccggga atggggacaa
      841 caaccgaccc tgaccacgtg gacgcccacc atggtgtggg tgctggccgg gacgaccacc
      901 ctgggcatcg tcgtgcaagg gctctgcctg atcgttccgc tgatgcgcag cgggttccgc
      961 taccggccac gattcggttg gcgcggttac ggtttcgggg acctgtcccg gatggcgggg
     1021 tggacgatcg cggcgctcac ggtgtcgcaa ctcggggggc tggtgacgac tcgggcgatg
     1081 accgtcggtg ccagccaagc cccgaacgtg gccggcaacg gcatccagca gtacgcctac
     1141 agcctctaca ccctgccgca ctcactcatc acggtctcga tcgtcacggc gctgttcccc
     1201 gccatgtcgc gggccttcca ggagggcgac ctcgcgggcc tgcgacggtg ggtggtcacg
     1261 gggctgaccg ctcccgcggt gctcgtcatc ccggcggcca ccgtgctcat cgccctgggg
     1321 cgcccgatgg tgtccgccct tttccctggg ctgcgagtcg tccctgcgct cggcgtggat
     1381 gagcccggca acgtggccct ggtgctggcc ctcatgtcca tcggcatcct gccgttcggc
     1441 atcactgccc tcaaacagcg ctactgcttc gcgcgcggcg acggctggct caacttctgg
     1501 caggtcgggg tgatgaccct cggcaacctc atcaccgcag tggtcgccat ggtggccacg
     1561 cctcccgagt atgtcgtcgc cgtcgtggcc gccggtggca ccatctcgaa catcgtcggt
     1621 gcggggctgt tcctgctggt ggcccggcga caactcgacg ggctcgccct gcggggggtc
     1681 tggttcctct ggctgcggct gacggttgcg tcgggtgtcg cgggcctcgc ggcgtgggcg
     1741 gtggcgtcct acatcgcggc gcccacgagt cgatggctcc tgcaggccat cgccctgatc
     1801 gtcggcggcg tcctcttcgt cctcgccttc gtggtcgcct cgcgcctgct gcgcatcggc
     1861 gaggtcaccg aggtcctcac ccgtttgacc tcccgaatca ccggccggct cggagcccgt
     1921 tctgcgcggt gactgagccg atcaggcggg tgcggtgccg cgctcgacga gggctcgccc
     1981 ggccttgagg atcgtcgcct cggcgagggt ccgcagcgag tcgacgacgt ccacgcgctc
     2041 agacaggccc tcgcggtcat agaccacgga cagttcggtg cagccgaaga tgacgtgcga
     2101 gcaaccggcc tctcgggcga cgtcgatgct ggcatgcagc gcgcctaggt cgaccggccg
     2161 accggccttg acctggtcat agatgatcgc ggagatcgtg gcctgcagcg ggcgtggggg
     2221 agtccacgcg atccccccgc cacgcgtgat ggcctcgtgg taggtgccgg cgtggatcgt
     2281 cccctcggtg gcgaagacgg cgatgggccc gtcagccagg gccagggcga cgctggcggt
     2341 ctcctgcacg atggacagca actcgatgct gatggcgcgt tccaccggct cggtgaagat
     2401 gttcgccgag ttgcacggca ggaccaagaa gtcggcgccc atccgctcca accgctgggc
     2461 gtcggcgacc aacgctggcg tcgggtccgg ggcggttggg tcgagcaggg ccgccgtgcg
     2521 gtcgggtgtc gtggcgtgct gcaggacgat ggcgtcgatg tggtcctggt cgcggtcggc
     2581 cttcgtcagc cggacgagca ggtcgaggaa cgagaccgtc gctgcggggc caaggccacc
     2641 catcaccccg acgatggggc tggaccagcc ctcagcgctc acgtgccgct tccgatctct
     2701 ccgagcgggt cgtagtaacg gtggaacttg cggtcgtagc tgaggccgcc gagggcgacc
     2761 ttgacccatc gccgcgggtc cttctcggcc cggtagtgca gcgggttgcg caggcccccg
     2821 gcctggatga cctcgtggac cttctcccac aactcggggt gaccctgcag cgcccacttg
     2881 atggtgcgga ccgggacgac cgaatagatg gtctcagcgg tggactcgag gacggggccg
     2941 acggggccga gggtgtcgcg gatgtactcg tcgacgtagt aggacaccgc gttgtggccg
     3001 gtccccgtga cgtagtagtt cgaccggccc aaccgggggt tgagctcgaa gaacttcacc
     3061 gagccgtcgc gcgggtcgta cttgaggtcg aagttcgcat agccgaccca ccgcaactcc
     3121 gccaacaacc gcgtcgcctg ggcgaccacc tcgctgttga cgccggtgag aatggctgca
     3181 gggacgccca actggccggg ggagtgctcc tcgatgaggg tctgccccca ggacgcgaac
     3241 cgcacgttgg agtcgcggtc gcagtagcac gtgaggatcc gcatgcccgt gtcgtcgccg
     3301 gggatccggt cctgcaggac cagcttgtcg cggtacccgg ccgcgtggat gcgcgccacc
     3361 aactcctgca actcggcctc ggaggccacc tcgtgcacct tggcctggcc ctcgaaactc
     3421 accccatgcc acgccgtcgg cgcggtcgcc ttaaggatta gcgggaacgg gccgggtgcc
     3481 tgaacctcgt gccagtcatc gccccccagg tcgatgatgg cggtcgccgg cgtgctgacc
     3541 ccggccttct gggccacctc gtcgaacgtg tgcttgcggg tggcccgctc caacacctcg
     3601 acgccgacgt agggcacccg gaacccgggg cccacccgat cagcgacggc gacgaccttc
     3661 tccacgaggg tgtccgaggt cgcgagcagc aacatcggaa cggtgatgtc cgtgaacggc
     3721 gcccccgtga gggtgcgcac aagggtgtcc tcgtcgagca ggtcggggca gtggaccttc
     3781 tccatgatgg tggagtggtc cgtgacccag gtgtcgccgc cggtgacgac gagcgaccgc
     3841 accccataac cctcgtggaa ggcccgagcc agcgaatagg cgccgacgtg gccaccaagg
     3901 atgacgggga cgaactcggg agcggacccg ccgccgggtt cagcgattct tgtaaacacg
     3961 gaccaacgcc tccacgagcc gacctgccag cggtcgcagc cgcagggtga actccccggc
     4021 gtactccaac cgctgcgggt tccattgccg tttgaactct tccagaccct cggacgcgtc
     4081 gatgccaccg aagtcgaagg tcgcgacgcc cgcggcgctc gcgcgcttca tcaactccca
     4141 caccatgagg tagttgggcc gcaggttgcg gtagtcgttg gacgacgcgg catacaggta
     4201 actgccgcgc gagccacacg tggcgaagac cgaccccgcg aggtagggcc cgcgcggggt
     4261 gacggcctcg atcgcgtcca tctcggccac gagcgccgcg cagttggcga cgctctgctc
     4321 gagcgtctcg atctggcggt cctgcttctc cgaatggggt ttgctgcgca gcttgcccag
     4381 ctggctggtc gactcggccg ccaacttctg cgcggccgcc cgcgccagag cggggtcgag
     4441 gcgcaccagc gagatggtga tgttg
//
LOCUS       DAOMZY010000291         4465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_18368_length_4465_cov_4.887302, whole genome shotgun sequence.
ACCESSION   DAOMZY010000291 DAOMZY010000000
VERSION     DAOMZY010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18368_length_4465_cov_4.887302"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..1350)
                     /locus_tag="PLK64_13845"
     CDS             complement(190..1350)
                     /locus_tag="PLK64_13845"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPV80873.1"
                     /translation="MPVSRLQHIPGIGVDHVGDRADAAHDPELLRMENLDTDLRPPAV
                     AVERTKAAVDEDAANSYLPFQGGVSLRQAAAAHVGRRAGRSYDPSRECVITAGGLNGI
                     LNTLLATLEPGDEVVLVDPIYAGLVNRVRLAGGIPRFVPLTPTPTGWALDPQSLAEAV
                     TARTAAVLVMSPAMPTGSVLGRPHWDALAQALERHPAWLIYDAAMERIRFDTGERIHP
                     AQIPGLAERTITVGAASKELRMIGWRVGWVVGPASIIADINTVGLTNVVCQVGLAQEA
                     VAAALSAPDASADVEHAVDTWRQRRDVILRELSGLPVIPPDGGWSLLIDTTQLGMSPA
                     DASERLFTRGRVAATPMTGWGPSGEKYLRLVFANEPVERLGDLRSRFDAAWG"
     gene            1635..1880
                     /locus_tag="PLK64_13850"
     CDS             1635..1880
                     /locus_tag="PLK64_13850"
                     /inference="COORDINATES: protein motif:HMM:NF018599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HPV80874.1"
                     /translation="MLELRPNCECCDRDLPPGDTEAMICTYECTWCRHCVNDCLRGRC
                     PNCGGDLQRPVRPAAHLEQNPPSAQRVHQIGLHLADQ"
     gene            1960..2913
                     /locus_tag="PLK64_13855"
     CDS             1960..2913
                     /locus_tag="PLK64_13855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HPV80875.1"
                     /translation="MKIVIPGGTGQVGGILRRALAARGHEVVVLSRHAGALEPGVRHT
                     PWDGRSLGAWASEIDGADAVINLAGRTVSCRYTEENLRQMMDSRVESTRVVGEAIAKA
                     AAPPRVWLQMSTATIYADRRDAPNDESTGIIGGHEPGVPGYWEYSVRIAQTWEATQLT
                     ADVPETRKVQLRAAMVMSPDRGGVFDVLLRMVRLGLGGPVAGGRQYVSWIHDEDFVRA
                     VELLLVREELSGPVNLAAPEPLPQRDLMRVLRRAWGQPVGLPATKWMAEIGAFALRSD
                     TELLLKSRRVVPGRLLDEGLEFAYGQWGAAAEDLVRRVRSR"
     gene            complement(3055..3495)
                     /locus_tag="PLK64_13860"
     CDS             complement(3055..3495)
                     /locus_tag="PLK64_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPV80876.1"
                     /translation="MGVAFRNVDVDPSAPVERWPYEAIVTVIERGTLEDWLILIRAIT
                     ADPWGPVARQVEDYLSYEQPYGVGPLLTRAIARARRQAEATERALVAEEVGQLVEQSG
                     LSLTEFASRMGTSVSRLSTYRSGRVTPSAALMTRMRRVSRSQPR"
     gene            complement(3495..4178)
                     /locus_tag="PLK64_13865"
     CDS             complement(3495..4178)
                     /locus_tag="PLK64_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80877.1"
                     /translation="MLDDVLASAARLQEIVPGAVLVGGAAAAMYAGHRESFDHDHVLD
                     DLAERYAEVVEAIEATDGWVTSVRASSPPLTLLGSLDGVEAGLRQLRRTRAVEVEEVR
                     VGTRVLRVPTLPECLRIKAFLVVQRNQVRDYLDTVALADRLGVPDSAAVLADLDAYYR
                     DRSDGPDAVVTALVQRLSEPNPRDSRVTTQLAAYKGLVARWQQWGTVVDDCAALADAI
                     VERRSQGAA"
BASE COUNT          690 a         1466 c         1606 g          703 t
ORIGIN      
        1 cctgcctcgt gcgcacgccc gcagctcacg ctccaaccaa gagcctcatt tagttggaag
       61 ttggagcacg gtgtgcaagt tcagttggac cgtcagtggc gtggttcgcc gggtgcctgg
      121 cacgagctca agagcgagta gcgaaccctg cgtgagtgag cggtgggcac acggcatacc
      181 ccctcgttct tagccccagg cggcgtcgaa ccggctgcgc agatcgccca accgctccac
      241 cggttcgttg gcgaacacga ggcgcaggta cttctcaccg gacggccccc acccggtcat
      301 cggcgtggcc gcgacccggc cgcgcgtgaa caaccgctcc gacgcgtcgg cgggcgacat
      361 cccgagctgg gtggtgtcga tgagcaggga ccagcctccg tccgggggga tcaccgggag
      421 tccggacagc tccctcagga tgacgtcgcg ccgttgccgc caggtgtcga cggcgtgctc
      481 cacgtcggcc gaggcatcgg gggcggacag ggccgcggcc actgcctcct gggctagccc
      541 gacctggcac acgacgttcg tcagcccgac cgtgttgatg tccgcgatga tgctcgccgg
      601 ccccacgacc cagccgaccc gccagccgat catccggagc tccttggagg ccgccccgac
      661 cgtgatcgtc cgctcggcga ggccggggat ctgcgcgggg tggatccgct cgcccgtgtc
      721 gaaccggatc cgctccatcg cggcgtcgta gatcagccag gcggggtggc gctccagcgc
      781 ctgggccagc gcgtcccagt gagggcgccc gaggaccgag ccggtcggca tggccggcga
      841 catcacgagg acggccgccg tcctggccgt gaccgcctcg gcgaggctct gcgggtcgag
      901 cgcccagccg gtcggggtgg gggtcaacgg cacgaacctt ggtatgccgc ccgcgagcct
      961 cacgcggttg accaaccccg cgtagatcgg gtcgaccagc acgacctcgt cgcccggttc
     1021 gagggtcgcc agcagggtgt tgaggatgcc gttgaggccg ccggcggtga tcacgcactc
     1081 gcggctcgga tcgtagctgc gtcccgcgcg acggccgacg tgggccgcgg cggcctgtcg
     1141 gagggagacg ccaccctgga aggggaggta actgttcgcg gcgtcctcgt cgacggccgc
     1201 cttggtccgt tccacggcga cggccggggg acgcagatcg gtgtcgaggt tctccatgcg
     1261 cagcagctcg ggatcgtgcg ccgcgtcggc gcggtcaccc acgtggtcaa ccccgatgcc
     1321 ggggatgtgc tgaaggcgac tcaccggcat ggttcctcct caggttccgt ggacagcacg
     1381 ctactgatgc gggcggcata ccgtccctca gtcgcgaagt gcagtctcca ccgcgtctcg
     1441 acacgaggtg tcgagcaggg ctgacgggcc ggggtcagcg ttggccgcgg cgttgcctgg
     1501 ccgcgattgc catgtagtgg cccgctggat agtggactcc gtagcgctgc cagtggtctt
     1561 cgatgcggtc ctcgcagatc catcgcactt cgtaggtggt gcgctgcgta ggtcctgatt
     1621 gtcagactcc cgccatgttg gaactgcgtc ccaattgcga gtgctgcgac cgggaccttc
     1681 ctcccggcga cacggaggcg atgatctgca cctacgagtg cacctggtgc cgccactgcg
     1741 ttaatgactg tcttcggggt cgttgtccca actgcggagg ggatcttcaa cggccagtgc
     1801 gtccagcggc acatcttgag cagaacccac catcagccca acgcgtgcat cagatcgggc
     1861 tgcatctggc tgaccagtag cgcagcgcac gctggctccc ggtatgccgt caacgacgtc
     1921 agtccgtggt caagcacgcc acccacctac tctggcggca tgaagatcgt catccccggg
     1981 gggaccggtc aggtcggtgg gatcctgcgg cgggcgctcg ctgcgcgagg gcatgaggtg
     2041 gtggtgctca gcaggcatgc gggtgcgctg gagccgggcg tgcggcatac cccatgggac
     2101 gggcggagct tgggggcgtg ggcgagcgag atcgacgggg ccgacgccgt catcaatctg
     2161 gccgggcgaa cggtgagctg tcgctacacc gaggagaacc ttcggcaaat gatggattcg
     2221 agagtggagt ccacccgggt cgtgggggag gcgatcgcga aggctgccgc gccgccgagg
     2281 gtgtggctcc agatgagcac ggcgacgatc tatgccgacc gtcgggacgc gccgaacgat
     2341 gagtcgacag ggatcatcgg cggtcacgag cccggggtgc cgggatactg ggagtacagc
     2401 gtccggatcg cgcaaacctg ggaggcgacc cagctcacgg cggacgttcc ggagacgcgg
     2461 aaggtccagc tgcgggcagc gatggtgatg agcccggacc gcggaggcgt cttcgacgtg
     2521 ctgttgcgga tggtccggct ggggctcgga gggcccgtgg cggggggccg gcagtacgtg
     2581 tcgtggatcc acgacgagga ctttgtgcgt gcagtggagc tcttgttggt gcgggaggaa
     2641 ctctcgggtc ccgtcaacct cgcggcgccg gagccgctcc cgcagcgaga cttgatgcga
     2701 gtgctgcgtc gcgcctgggg gcagccggtc gggctcccgg ccacgaagtg gatggccgag
     2761 atcggggcct tcgcgttgcg aagcgacact gaactgctgc tcaagagccg acgagtggtg
     2821 ccggggaggc tgctcgacga gggcttggag ttcgcgtacg ggcagtgggg tgcggcggcc
     2881 gaggacctcg tccggcgcgt tcggtcgcgt tgagccagcg gtgggcacac ggcataccaa
     2941 tggttggcgg tcacccgcgc ccacgtgctc accgccctgc cgcgccgtgg accattggta
     3001 tgccgtgtgc gccgggcgac gcaggcaccg accgcagaac gggacgacgc cgactcagcg
     3061 cggctgcgag cgggacactc gccgcatccg ggtcatcagg gccgcggagg gagtcacccg
     3121 ccccgaccgg taggtcgaga gtcggctgac cgatgtcccc atccggctgg cgaactcggt
     3181 gagcgacagg cccgactgct cgacgagctg accgacttcc tcggcaacca acgcccgctc
     3241 ggtggcctcc gcctggcgcc gagcccgggc gatcgcccgg gtcagcaacg gcccgacgcc
     3301 gtagggctgc tcgtaggaga gatagtcctc cacctgccgc gccaccgggc cccatgggtc
     3361 ggcggtgata gcccggatga ggatcagcca gtcctccagg gtgccccgtt cgatgacggt
     3421 gacgatggcc tcatacggcc agcgctcgac gggcgcgctc gggtcgacgt cgacattgcg
     3481 gaacgcgacg cccatcaggc agcaccctgc gagcggcgct cgacaatcgc gtcggccagc
     3541 gccgcacagt cgtccacgac cgtgccccac tgctgccacc gggcaaccag gcccttgtat
     3601 gccgccaact gggtcgtcac tcgggagtcg cgtgggttgg gttcggatag ccgctggacc
     3661 agagcggtca cgacggcgtc cggtccgtca gaccgatcgc ggtagtacgc gtcgaggtcc
     3721 gcgagaacgg cggcggagtc ggggactccg agccggtcgg cgagggccac cgtgtcgagg
     3781 tagtcgcgga cctgattgcg ctgcacgaca agaaaggcct tgatgcgcag gcactctggc
     3841 agagtcggaa cgcgcaggac acgtgtgccg acgcggactt cctcgacctc gacggctcgc
     3901 gtgcggcgca actgacgcag cccggcctcg actccgtcga gcgacccgag cagcgtgaga
     3961 ggaggggagc tggcgcgcac gctggtgacc cagccgtccg ttgcctcgat cgcctcgacg
     4021 acctcggcat atcgctcggc caggtcgtcc aggacgtgat cgtggtcgaa ggactctcgg
     4081 tgaccggcat acatggccgc cgccgcaccg cccacgagca ccgctccggg cacgatctcc
     4141 tgcaaacggg cggcggaggc gagaacgtca tcgagcatcg ccactcgatg atcttatcag
     4201 aaccgataac atccggcccc aggtttgacg tttcggactg ccgctctcga cgccttcgcc
     4261 gtcggcttgg acttccggat accgtcggcg aacctgtcgg cttccccgac atgttcccaa
     4321 gacgtgtgag gtttcgtcgc gattcttaac acgttgggcg gccgctggtt gggtgtcaac
     4381 cggctggctg tgctcgtgga gctagtccgc gcgggtgagg acctgcagca gatcacggtt
     4441 gaggaagacg acgtcgcggc cgatc
//
LOCUS       DAOMZY010000292         4451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_18448_length_4451_cov_5.372157, whole genome shotgun sequence.
ACCESSION   DAOMZY010000292 DAOMZY010000000
VERSION     DAOMZY010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18448_length_4451_cov_5.372157"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="PLK64_13870"
     CDS             complement(<1..1155)
                     /locus_tag="PLK64_13870"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPV80878.1"
                     /translation="MTSTDLRVDIEEVRTKQDRLAAARAMLHTHFVGIDAVIDELCDA
                     IAVWYLMPEVLSRPLVVNLWGMTGVGKTDLVRRLVAALGLQERFVELELSSTDAQSRF
                     DLVADRLADAGVLDGRPAVLLVDEIQRFRTITPSGDPVAQTRFSDFWELLSDGRLARR
                     ESSDLDWLIATMRAARDEDARREGKGKSKGKRPSIGLWEARSLRRTLGLAEPVEQLQW
                     RTHDEALAMLESARAERRLVEPVDCSRFLVIVSGNLDEAFTMASATGEADIDADIFAA
                     HTARVGMVDIKAALRQRFTPEQVARFGNVHVIYQSLRRADFQTLVAREIARLCDAAYR
                     RFGVRVAVTPAVHELVYRNGVFPVQGVRPVLSSVGDIVGTPLATLLMDAVL"
     gene            complement(1170..1916)
                     /locus_tag="PLK64_13875"
     CDS             complement(1170..1916)
                     /locus_tag="PLK64_13875"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPV80879.1"
                     /translation="MRTRLVLVHGSRVSGVQWSAHRRWLEPEFDVITPDLPSHGTRAA
                     EPFTMDAAVAAVAEAVDEAAPDQPVVLVGHSLGGYVAMTYAAAYPRRLAGLVTIGAAG
                     VPQGLGAAAYTLLGNVTEKAGRERITAINDRMLRRMAAPEVFEAIAADGYWFEGVQPS
                     WAAVMAGGRPELLSGVECPVLIVRGQFDQLGINARRYAAAAPNARIVTVPRASHLLPL
                     TRPWQTSAIIANFAREVTGTENRWSADSPA"
     gene            2152..3675
                     /locus_tag="PLK64_13880"
     CDS             2152..3675
                     /locus_tag="PLK64_13880"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HPV80880.1"
                     /translation="MDSSEATRDSASCWAAVKAGTAAVTELVGLLWQVQGADLGALLE
                     ALDAMTAVAGGARVAVVAEAESRGEISASQAGSTAGWVAEHAPTLAAGGGAGQVARLV
                     RECARPDLRSVQAAAVSGAVSVPAALTVVTEYARLKPRLREEAAPTVLQGLLDMGIAD
                     GPKGVRSLRPALLAAHGADGEFQADADTAERLVSLSRPSTDELGALTYQLILDPAGAA
                     ALESAIGPLSRPVPGPDGERDPRSPQVRRGQALIEVCRRATSAGDSPPSGVKTTLMLT
                     MSYEDLAARTGAATVVGSTQGGSLIAPETVRRLACDANVIPTVLGSRGEVLDLGRSTR
                     LATPGQLAALWLRDRGCSFPGCTTPAHWCDAHHLVHWADGGTSGLTNLALLCGRHHSV
                     VHRDHLIGHITGASAVTGAVTWNVIPGSYDRRHHAIPPPGDPRPGGQPPCDQPPGDSP
                     PPLNRPAPDQHEWDNPAPDADPWADLWQPSGRSSTGEPEVPDANPEANPWHDSGPAA"
     gene            complement(3766..>4451)
                     /locus_tag="PLK64_13885"
     CDS             complement(3766..>4451)
                     /locus_tag="PLK64_13885"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019483536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPV80881.1"
                     /translation="RWNWYLDLGLSADNLRLYEHAKEKLSHYSKRTVDIEYRFNFTGS
                     EWGELEGIANRTDFDLSSHSQHSGQDLSYFDQATGERFVPYVIEPAAGLSRSLMTFLV
                     DAYAEDEAPNTKGGVDKRIVLRLDPRLAPVKAAVLPLSRNADLSPKARDLAATLRRNW
                     NVEFDDAGAIGKRYRRQDEIGTPYCITVDFDTLEDHAVTIRERDTMSQERISIDQVQG
                     WLAQRLVGC"
BASE COUNT          691 a         1517 c         1547 g          696 t
ORIGIN      
        1 gagcacggca tccatgagca gggttgccaa gggagttccc acgatgtcgc cgacgctgga
       61 gaggaccggc cgcacccctt gaacggggaa aacgccgttg cgatagacca attcgtggac
      121 agcgggagtc accgccacgc ggaccccgaa gcgccggtat gccgcgtcgc acagtcgcgc
      181 gatctccctc gcgacgagcg tttggaagtc ggcgcgacgc aggctctggt agatgacgtg
      241 gacgttgccg aacctcgcga cctgctcggg cgtgaaccgc tggcgcaggg ctgccttgat
      301 gtcgaccatc cccacgcgtg cggtgtgggc ggcgaagatg tcggcgtcga tgtcggcctc
      361 accggtggca ctggccatgg tgaacgcctc gtcgaggttc ccactgacga tgaccaggaa
      421 ccgcgagcag tccacgggct ccacgagacg gcgttcggcc cgggcgctct cgagcatcgc
      481 caacgcctcg tcgtgcgtcc gccattgcag ttgctcgacc ggctcggcca atcccagtgt
      541 gcggcgcaga gatcgggcct cccagagacc gatgctcggc cgcttgccct tggacttgcc
      601 cttaccctcg cggcgcgcat cctcatcgcg ggcggcgcgc atcgtcgcga tcagccagtc
      661 gaggtcggag ctctccctgc gggccaggcg gccgtcgctg agcaactccc agaagtcgct
      721 gaaccgcgtc tgggcaacag ggtcaccact cggggtgatg gtgcggaagc gctggatctc
      781 gtcgacgagc aacaccgccg gccggccatc gaggacgccg gcatcggcga ggcggtctgc
      841 gacgaggtcg aaccgcgact gcgcgtcagt gctggacaac tcgagttcga cgaaccgctc
      901 ctgcaacccc agagctgcga cgagacgccg gaccagatcg gtcttgccga ccccggtcat
      961 cccccagagg ttgacgacga gcgggcgaga gagcacctcg ggcatgagat accagacggc
     1021 gatggcgtcg cagagctcat cgatgacggc gtcgatcccg acgaaatggg tgtgcagcat
     1081 cgcccgagca gccgccagcc ggtcctgctt ggtcctcacc tcctcgatgt cgacccgaag
     1141 gtccgtcgac gtcacaatca accatcgtgt caggccggcg agtctgcgct ccaacgattt
     1201 tcggtgccgg tgacctcgcg ggcgaagttc gcgatgatcg ccgaggtctg ccacggacgg
     1261 gtcagcggca ggaggtgcga ggcgcgcggg accgtgacga tgcgcgcgtt cggcgcggcg
     1321 gcggcatacc ggcgggcgtt gatgccgagt tggtcgaact ggccgcgcac gatgagcacg
     1381 gggcactcca cgccactgag caactcgggt cgcccgcccg ccatcaccgc cgcccacgac
     1441 ggctgcaccc cctcgaacca gtacccgtcg gccgcaatcg cctcgaacac ctcaggcgcc
     1501 gccatccgcc gcagcatccg gtcgttgatc gccgtgatcc gctctcgccc cgccttctcg
     1561 gtgacgttcc ccaggagggt gtatgccgcg gcgcccagcc cctggggcac ccccgccgcc
     1621 ccgatcgtca cgagtccggc caaccgcctc gggtatgccg cggcatacgt catcgcgaca
     1681 tagccaccga ggctgtggcc gacgaggacg accggctggt cgggcgcggc ttcgtcgaca
     1741 gcctccgcga cggcggccac tgcggcatcc atcgtgaagg gttcggccgc ccgggtgccg
     1801 tggctgggga ggtcgggggt gatgacgtcg aactcgggct ccagccagcg ccgatgggcc
     1861 gaccattgca cgccgctcac tcgagagccg tggacgagga cgaggcgggt gcgcacgctc
     1921 tcaggctacg tgccgcgcat ggcactaccg cagagcggct gggcggcgag gtctccgaca
     1981 tccagcgacc gcttcaaact cttcggcggg ccctgcattc ggtgctcgcc gaacccgggt
     2041 tagcccgcga acgaccgagc tgtgatcgtt acctgcttgt gatgtgttct gttgtgggag
     2101 aggccttttg gggtgggggg tctgtcagtg gtcgctgtta gcgtcggggc tatggacagc
     2161 agcgaagcga ctcgtgactc ggcatcatgc tgggctgcgg tcaaggctgg aaccgccgct
     2221 gtcacggagt tggtgggcct gttatggcag gtccagggag ccgacttggg ggccctgctc
     2281 gaagcgttgg acgcgatgac cgcggtcgct ggtggcgctc gggtcgcagt ggtggcggaa
     2341 gccgagtctc gcggtgagat ctccgcgtcg caggcggggt cgacggctgg gtgggtggct
     2401 gagcacgcgc cgactctggc ggccggcggt ggtgccggtc aggttgcccg tctcgtccgt
     2461 gagtgtgctc gcccggacct gaggtccgtc caggccgcgg ctgtctcggg ggcggtgagt
     2521 gtgccggccg cgttgacggt ggtcaccgaa tacgcccgcc tgaagccacg tctgcgggaa
     2581 gaagccgccc cgaccgtcct gcaagggttg ttggacatgg ggatcgcgga cggaccgaag
     2641 ggtgtccggt cgctgcgtcc cgcattgctg gcggcacacg gcgctgatgg ggagttccag
     2701 gctgatgcag acaccgccga acgcctcgtc tccctgtctc gcccgtcgac agacgagctc
     2761 ggcgccctca cctaccagtt gatcctggac cccgccggag cggccgccct cgaatcagcc
     2821 atcggcccct tgtcgaggcc ggtccccggc cccgatggtg aacgcgaccc gcgcagccct
     2881 caggtgcgtc gtgggcaggc gctgattgag gtatgccgcc gcgccaccag cgccggcgac
     2941 agcccaccgt ccggggtgaa gaccacgttg atgctgacga tgtcttacga ggacttggcg
     3001 gctcgcaccg gcgcggcgac cgtggtcggc tcaactcaag gcggttcgct gatcgcgccc
     3061 gagacggtgc gtcggttggc gtgcgacgcg aacgtcatcc ccaccgttct gggtagccgc
     3121 ggcgaggtcc tcgacctcgg ccgatccacc cggctcgcga cacccggaca gttggcggcg
     3181 ttgtggttac gggaccgtgg gtgcagtttc cccggctgca cgaccccggc acattggtgc
     3241 gacgcccacc atttggtcca ctgggccgac ggcggcacgt ccggcctcac caacctcgcc
     3301 ctgttgtgcg ggcgacacca ctcggtcgtc caccgcgacc acctcatcgg ccacatcacc
     3361 ggcgcgagcg ctgtcactgg ggctgtcacc tggaacgtca tccccggcag ttacgaccgc
     3421 cgacaccacg cgatcccacc acccggcgat ccgcgaccgg gtggccaacc accatgtgac
     3481 caaccgccgg gtgactcgcc cccgcccctg aaccgtccag cacccgacca gcacgagtgg
     3541 gacaaccctg cccccgacgc agacccctgg gccgacttgt ggcagcccag cggccgatcg
     3601 tcaaccggcg aacccgaggt gcccgacgcc aaccccgagg caaacccttg gcacgacagc
     3661 ggcccggcgg cctgaggtgc gcctcagcgg catacaggaa ggcgcagagc cgaaccgcac
     3721 ggagtcagca gccagcagcc agcagtggtc agcaagcggc agggctcagc agccgacgag
     3781 acgctgggcc agccagccct gaacctggtc gatgctgatc cgctcctgcg acatggtgtc
     3841 gcgctcgcga atggtcacgg cgtggtcctc gagcgtgtcg aagtcgaccg tgatgcagta
     3901 gggcgtgccg atctcgtcct ggcggcggta gcgcttgccg atcgcaccgg cgtcgtcgaa
     3961 ctcgacgttc cagttgcggc gcagcgtcgc cgcgaggtcg cgggccttgg gcgagaggtc
     4021 ggcgttgcgc gacagcggca ggaccgcggc cttgaccggg gccagccgcg ggtccaaccg
     4081 caggacgatc cgcttgtcca ccccaccctt ggtgttgggg gcctcgtcct cggcgtaggc
     4141 gtcgacgagg aaggtcatca ggctgcgcga caggcccgcc gccggctcga tgacgtaagg
     4201 aacgaagcgc tcaccggtcg cctggtcgaa gtaggacagg tcctgcccgg agtgctggct
     4261 gtgcgacgac aggtcgaagt cggtccggtt ggcgatgcct tcgagctcgc cccattcgga
     4321 gccggtgaag ttgaagcggt actcgatgtc gacggtgcgc ttcgagtagt gcgacagctt
     4381 ctccttcgcg tgctcgtaga ggcggaggtt gtcggcggac aggccgaggt cgaggtacca
     4441 gttccagcgg t
//
LOCUS       DAOMZY010000293         4446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_18474_length_4446_cov_5.292189, whole genome shotgun sequence.
ACCESSION   DAOMZY010000293 DAOMZY010000000
VERSION     DAOMZY010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18474_length_4446_cov_5.292189"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(286..1632)
                     /gene="ccrA"
                     /locus_tag="PLK64_13890"
     CDS             complement(286..1632)
                     /gene="ccrA"
                     /locus_tag="PLK64_13890"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR01751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HPV80882.1"
                     /translation="MTKELYDLGEQPPLGEIPAKMHAWLIRPERFGPPLEAFQKEVVD
                     VPTIADDEVLVYVMAAGINYNNVWAGLGIPVDVVGARNKAGEPERFHIGGSDASGIVY
                     AVGKDVTNVKVGDEVVMHCGKWDRDCPTVQRGGDPMYSPSFRIWGYETNYGSFAQFTK
                     VQGQQCMPKPTHLTWEAASAYTLVAATAWRMLHGWGENAVKEGDVALIWGGAGGLGSM
                     AIQIVKAAGGIPIAVVSGEDKFDYCMKLGAKGCINRNDFDHWGMLPHWKDDAAYGTWL
                     KGARKFGSAIWDVLGERRAPNIVFEHPGESTIPSSIFVCDTGGMVVVCAGTTGYNATV
                     DLRYLWMRQKRLQGSHFANSVQANQMNQLALAGQLDPCLSQAFTYDEIPLAHDLMYRN
                     KHPHGNMAVLVGATDFGQGITAPEPVAMPHPTLPDGDAHTTPRILPHATDSELLPE"
     gene            complement(1828..2265)
                     /gene="mce"
                     /locus_tag="PLK64_13895"
     CDS             complement(1828..2265)
                     /gene="mce"
                     /locus_tag="PLK64_13895"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HPV80883.1"
                     /translation="MDAPLFTMIDHVGIAVADLDAAIAFYRDTYGMALLHEETNVEQG
                     VREAMMAVGHSGSCIQLLAPLSPESPIGKFLTRSGPGIQQMAYRVEDIDAVCATLRER
                     GVRLLYDVPKRGTSDSRVNFIHPKDAGGVLVELVEPATDTPHH"
     gene            2697..3893
                     /locus_tag="PLK64_13900"
     CDS             2697..3893
                     /locus_tag="PLK64_13900"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HPV80884.1"
                     /translation="MSSNTSVIVAGARTGMGRLLGSLKDFSATDLGGIAIKGALEKSG
                     ITGDQVDYVIMGHVLQAGCGQVTARQAAVKGGIPMDVPSITINKVCLSGINAIATADQ
                     LIRSGECEIVVAGGMESMTNAPHLLEKSREGFKYGTVKMRDHMDYDALWDVYTDQGMG
                     NLTESANSGDREFTREEQDAFSARSHELAAKAWKDGLFDDEVVPVMIPQRKGDPIEFK
                     ADEGVRAATTAESLAGLRPAFRKDGTITAGSASQISDGAAAVVVMSKAKAEALGLTWL
                     AEIGAAGYVAGPDSTLQSQPAHAIVKACAKEGITPADLDLLELNEAFAAVGLASTKEL
                     GVDPDKVNVNGGAIAMGHPVGMSGARIVLHLANELKRRGGGVGAAALCGGGGQGDALI
                     VRVPKA"
     gene            4030..>4446
                     /locus_tag="PLK64_13905"
     CDS             4030..>4446
                     /locus_tag="PLK64_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPV80885.1"
                     /translation="MPPRPVDVPALVTAARAGSPRAVARLISLVEDAHPALREVMAAL
                     APHTGKAHVIGLTGSPGVGKSTSTSALVAAFRARGKRVGILAVDPSSPFSGGALLGDR
                     VRMQEHALDPEVYIRSMASRGHLGGLAWTTPQAIRVL"
BASE COUNT          661 a         1525 c         1538 g          722 t
ORIGIN      
        1 cggcgccggg gttggcacag ggatcaccga tccctcaccc gccccgtcct cgagcgtgaa
       61 ctcgggttcc tccgacagtc gggcgcgctg gcgttgccgg aagtccagcc aacgctgcca
      121 caccgagccg gtgtcgccca tcacccacct ccacacgccg acgacgtatc cacgatcgtc
      181 gagcctaagg gagccgtgtc ccgcaggcgc ggcggcgcgg catacccggt cggtatgccg
      241 cgccgagacg ctcgtccgct acgcgcctgc gcgcgcagcg ccgggtcact ccgggagcaa
      301 ctcgctgtcg gtcgcgtggg ggaggatccg gggcgtcgtg tgggcgtcac cgtcgggcag
      361 ggtcgggtgc ggcatggcca ccggctcggg tgcggtgatg ccctgaccga agtccgtggc
      421 gccgacgagg accgccatgt ttccgtgcgg gtgcttgttg cggtacatga ggtcgtgggc
      481 cagggggatc tcgtcgtagg tgaaggcctg cgacaggcac gggtcgagct gcccggccaa
      541 cgccaactgg ttcatctggt tggcttgaac ggagttggcg aagtgcgagc cttgcaggcg
      601 cttctggcgc atccacaggt agcgcagatc gacggtcgcg ttgtagcccg tggtgcccgc
      661 gcagacgacg accatgccgc cggtgtcgca gacgaagatt gagctgggaa tcgtgctctc
      721 gcccgggtgc tcgaagacga tgttcggtgc gcgccgctcg cccaggacgt cccagatcgc
      781 cgaaccgaac ttgcgcgcac ccttgagcca ggtcccgtat gccgcgtcgt ccttccagtg
      841 gggcagcatc ccccagtggt cgaagtcgtt gcggttgatg cagcccttgg cgccgagctt
      901 catgcagtag tcgaacttgt cctcgcccga aaccacagca atgggtatgc cgccggccgc
      961 cttgacgatc tgaatcgcca tcgagccaag gccgcccgcg ccaccccaga tgagggcgac
     1021 gtcgccttcc ttgacggcgt tctcgcccca gccgtgcagc atccgccacg ctgtcgctgc
     1081 gacgagggtg taggccgagg cggcttccca ggtcaggtgt gtgggcttgg gcatgcactg
     1141 ctggccctgg accttggtga actgggcgaa gctgccgtag ttggtttcgt agccccagat
     1201 ccggaagctc ggcgaataca tcgggtcgcc gccgcgctgg accgtggggc agtcgcggtc
     1261 ccacttgccg cagtgcatga cgacctcgtc gccgaccttg acgttggtga cgtccttgcc
     1321 gacggcatac acgatgcccg aggcgtcgct gccgccgatg tggaagcgct ccggctcgcc
     1381 tgccttgttg cgggcgccga cgacgtcgac cgggatgccg aggccggccc acacgttgtt
     1441 gtagttgatg ccggctgcca tgacgtagac gagcacctcg tcgtcggcga tcgtcgggac
     1501 atcgacgacc tccttctgga aggcctccag cggcgggccg aagcgctcag ggcggatcag
     1561 ccaggcatgc atcttcgcgg ggatctcgcc caggggaggc tgctcgccca ggtcgtagag
     1621 ttccttggtc atcgtgggtc ctctctgcaa aggcgggccg cccgcgggct gggcggccag
     1681 cgctggagat cgacgacgcg ccaacttttc tggcgctctt cgctcgaccg tactcgcaag
     1741 ccgaaggcct gctattgggc acaacccgtg acgttgccca cctgagaagc gtggcggcgc
     1801 agcaccgggt cgggtggtga gcgaccgtca gtgatggggt gtatcggtgg cgggctcgac
     1861 gagctcgacg agcaccccgc cggcgtcctt ggggtggatg aagttgaccc ggctgtcgct
     1921 cgtcccacgc ttggggacgt cgtagagcag ccgcacaccg cgctcgcgca gggtggcgca
     1981 gacggcgtcg atgtcctcga cccggtaggc catctgctgg atccccgggc cggagcgcgt
     2041 gaggaacttg ccgatcggcg actccggcga caagggggcg agtagttgga tgcacgaccc
     2101 cgagtgcccg acggccatca tcgcctcgcg caccccctgc tccacgttgg tctcctcgtg
     2161 cagcagggcc atgccgtagg tgtcgcggta gaaggcgatg gccgcatcga ggtcggcgac
     2221 ggcaatgccg acgtggtcga tcatcgtgaa gaggggggcg tccatacctc tgagcctacg
     2281 gccgcggcat accgtcgggc acgccggaag ggcctcgaag gcggcggtgt gacggccttc
     2341 atgctgccgc cgacccgcct gcggataccg cagttgagtc actctcagac aacgaaaatc
     2401 gacagtctcg gcaagagttc cgttcgaatc ctcggaaaac tctggcgctc acccggtctc
     2461 gccgtctacc gtccgacccg agcctgtgcc gcaggaggga acgctcaggc cccggaccct
     2521 ggggtggggg attcgcgcgg cataccggag ggccgaccgc ccgccggtat gccgcgcggt
     2581 cgcacctgtg tgtggtgcgt cccaccagag ctccccgggt ccaccgtcac tcgccgcgga
     2641 tacagtgatc gatatctgac gtctctcgct cctgatcgcc ccctggaggc tgcgctatga
     2701 gctcgaacac ctctgtcatc gtcgctggtg cccgcaccgg catgggccgg ctgctcggct
     2761 cgctcaagga cttctcggcg accgacctgg gcggcatcgc catcaagggc gccctggaga
     2821 agtccggcat cacgggcgac caggtcgact acgtcatcat gggccacgtc ctgcaggccg
     2881 gctgcggcca ggtgacggcg cgccaggcgg cggtcaaggg cggcatcccc atggacgtcc
     2941 cgtcgatcac gatcaacaag gtctgcctgt cgggcatcaa cgccatcgcc acggccgacc
     3001 agctcatccg ctcgggtgag tgcgagatcg tcgtcgctgg cggcatggag tcgatgacca
     3061 atgcgcccca cctgctggag aagagccgcg agggcttcaa gtacggcacg gtcaagatgc
     3121 gcgaccacat ggactacgac gccctgtggg acgtctacac cgaccagggc atgggcaacc
     3181 tcaccgagtc ggccaacagc ggtgaccgcg agttcacccg cgaagagcag gacgccttct
     3241 ccgcacgcag ccacgagttg gccgccaagg catggaagga cggcctgttc gacgacgagg
     3301 tcgtccccgt gatgatcccg cagcgcaagg gcgacccgat cgagttcaag gccgatgagg
     3361 gcgtgcgcgc cgcgacgacg gccgagagcc ttgccgggct gcggccggcc ttccgcaagg
     3421 acggcacgat caccgcgggc agcgcctcgc agatctccga cggggccgct gcggtcgtcg
     3481 tcatgagcaa ggcgaaggcc gaggctctcg ggttgacctg gctggccgag atcggcgctg
     3541 ccggctatgt cgctggcccc gactcgacgc tgcagtccca gcccgcccac gcgatcgtca
     3601 aggcgtgcgc caaggagggc atcacgcccg ccgacctcga cctgctcgag ctcaacgagg
     3661 ccttcgcggc cgtcggtctg gcctccacca aggagctggg cgtcgacccc gacaaggtca
     3721 acgtcaatgg cggcgccatc gccatgggcc acccggtcgg catgtcgggc gcccggatcg
     3781 tcctgcacct ggccaacgag ctcaagcgcc gcggcggcgg cgtcggtgcg gctgccctct
     3841 gcggcggcgg cggccagggc gacgcgctca tcgtgcgggt cccgaaggcc tgatcttctc
     3901 ccgggggaac cgcacggcgt gctcccgggt tgtcatcgtg tatgccgcgc ggctgcccga
     3961 ccccctgcgg gcaccgcgcg gcataccctg tcggtcgccg tgtccttcac cccgccccga
     4021 gaggttggta tgccgccccg cccggtcgat gtccccgcac tggtcaccgc tgcccgagcc
     4081 gggtcacccc gcgccgtcgc gcgcctgatc tcgttggtcg aggatgccca tcccgcactg
     4141 cgtgaggtga tggcggcgct ggcccctcac acgggcaagg cccacgtcat cggtctcacg
     4201 ggctctcccg gggtcggcaa gtcgacctcg acgagcgcgt tggtggcggc gttccgggcc
     4261 cgtggcaagc gggtggggat cctcgcggtc gacccgtcct cgccgttctc cggtggtgcg
     4321 ctgctcggtg accgggtgcg gatgcaggag cacgcgctcg accccgaggt ctatatccgc
     4381 tcgatggcct ctcgcgggca ccttggtggg ctggcgtgga ccacgccgca agcgatccgg
     4441 gtgctc
//
LOCUS       DAOMZY010000294         4394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_18764_length_4394_cov_5.735423, whole genome shotgun sequence.
ACCESSION   DAOMZY010000294 DAOMZY010000000
VERSION     DAOMZY010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18764_length_4394_cov_5.735423"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PLK64_13910"
     CDS             complement(<1..586)
                     /locus_tag="PLK64_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HPV80886.1"
                     /translation="MSPILIAADLLAIALLVFGMFLPRHGRRDLVAAYLGVNVGVFAV
                     AAALTASGVASAGVGLGLFGVLSIIRLRSEELTQTEIAYYFGALAIGLLAGLGADAVP
                     MIGALMALVVVTLWIGDHPRIATRSVRQTLVLDRAHGDRSALHAHVERVLGGTVHEIS
                     VVKTDLVNDTTLVSVRHTPLPLVGRAGSVRPGGGG"
     regulatory      753..883
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            996..1709
                     /locus_tag="PLK64_13915"
     CDS             996..1709
                     /locus_tag="PLK64_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0034257 - nicotinamide riboside
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter family
                     protein"
                     /protein_id="HPV80887.1"
                     /translation="MNNGNVFQQLIAATFPVGGYQMHWLELIGILIGAASAYLGMKRW
                     VWAWPVGILANVMLFFVYLGALFGADERIPLFGQAGRQIFFIVTSLYGWWRWNESKRA
                     RGVDNAGPAITPRWATSRERIGIVVGWLIGTVIVHQAFVALSSWPAQWWFYWCDAWIF
                     VGSVLATYSMARGWNEFWLCWIAVDLIGVPLGFATGYVPTAVLYIGYGAFVIYGFTQW
                     VKATREESGSAPAESPAVR"
     gene            1860..3185
                     /locus_tag="PLK64_13920"
     CDS             1860..3185
                     /locus_tag="PLK64_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HPV80888.1"
                     /translation="MATLRSGRGGLAGHRDAFLTSGDAGGGVRSVVADSWMRSSASGV
                     DPDRHLAPIRMDRPEVQTYRSAHPLAQVFPLLYDVLGRAAVDCDCVMAVGDADGTLLW
                     VTGSPTVMRRAETIHFVEGAGWDEGHAGTNAPGMALHLDAATHVRSEEHFNRLVQPWT
                     CAAAPIHDPSTQQILGLVDITGGPDVATPQSLALVRAAARMAESELARLQATGALWTP
                     AAPGRRGSGDASLGPDPSAAFALRGLGQPELIAAHGGRTHRLSRRHGDILVALADHPD
                     GLTAEQLEVLVYAGQVTSSTMRAEMTRLRSILGPEVLTSRPYRLIAGAQTDWHAVAAY
                     LAAGRLRAAMQAYRGPLLPMSQSPAVVDQREILTGQVRSALIASAEADLMVAWTRSRW
                     GADDLDMWERQAETLPAGSPLRPIAVAEAARLERELVAAPIVLGRQTRA"
     gene            3323..>4394
                     /locus_tag="PLK64_13925"
     CDS             3323..>4394
                     /locus_tag="PLK64_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015937949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPV80889.1"
                     /translation="MTIFAPPGSDGSPVSVASRYDNFIGGQWVPPVKGQYFENVTPVT
                     GRVFTEIARSTAEDIELALDAAHGAAPAWGRTSAADRAVVLNKIADRIEANLADLALA
                     ETWDNGKPIRETLAADLPLAVDHFRYFAGAIRAQEGGISEIDADTIAYHFHEPLGVVG
                     QIIPWNFPLLMATWKLAPALAAGNAVVLKPAEQTPWSILKLMELIADLLPDGVLNVVN
                     GFGVEAGKPLASSSRIAKIAFTGETTTGRLIMQYASQNLIPVTLELGGKSPNIFFSDV
                     ADERDAFYDKALEGFAMFALNQGEVCTCPSRALIQSSIYDNFLIDAVERVGKITQGNP
                     LDTDTMIGAQASNDQLEKILSYI"
BASE COUNT          668 a         1544 c         1475 g          707 t
ORIGIN      
        1 acccccctcc ccctggtcgc acgctgccgg cgcgtccgac cagggggagg ggggtatgcc
       61 gcaccgagac gagcgtggtg tcgttgacca ggtcggtctt cacgaccgag atctcgtgga
      121 cggtgccgcc gaggacgcgc tcgacgtggg cgtgcagggc cgaacgatct ccgtgcgccc
      181 ggtccaggac cagcgtctgg cggacgctgc gggtggcgat ccgcgggtgg tccccgatcc
      241 acagcgtcac cacgaccaag gccatgaggg cgccgatcat cgggacggcg tcggcgccga
      301 gcccggccag cagcccgatc gcgagggcgc cgaagtaata ggcgatctcg gtctgggtga
      361 gttcctcgga ccgcagccgg atgatggaca ggaccccgaa gaggccgagc ccgacaccgg
      421 cgctggccac ccccgatgcg gtgagggcgg cggctacggc gaataccccc acgttgacgc
      481 cgaggtaggc ggcgacgaga tcacgtcggc cgtgccgagg caggaacatg ccgaagacga
      541 gcaaggcgat agccagaagg tcggcggcga tgaggatggg ggacatggtg ggctccgtgt
      601 gtggttgtga cgcgcggtgg cttgatgtcc ttagtcagcc gtcccgagtt gttccgatcc
      661 ggtgaggtca ctgtgggacg tctgtgagac gttgccgggt ccgcgtggtg gtcgggcgcc
      721 tggacccggc gtgctcgcgg catacactgt gggcacgtgc tccggggtcg gtgtaactcc
      781 gaaccggcgg tgacagtccg cgacccgacc gcatccagcg gccggttgac ctggtgggat
      841 tccaggaccg acggtgaaag tccggatggt aggcagcacg tttcgtggcg cgacgacgcc
      901 cggtatgccg ctgtgtcggc ttccggggcc ggcgtggtac ggggtgtccc cggagttcgt
      961 ccctgtcaga acgaactcaa ggacgaacac caatcatgaa caacggcaac gtttttcagc
     1021 agctcatcgc tgcgacgttc ccggtcggtg gctatcagat gcactggctg gaactcatcg
     1081 gcatcctcat cggtgccgcc tccgcatatc tgggcatgaa gcgctgggtc tgggcctggc
     1141 cggtcggcat cctcgcgaac gtcatgctct tcttcgtcta cctcggagcc ctgttcggcg
     1201 ccgacgagcg gatcccgctg ttcggccagg ccgggcggca gatcttcttc atcgtcacga
     1261 gcctctacgg gtggtggcgg tggaacgagt ccaagcgggc tcgtggcgtc gacaacgccg
     1321 gaccggcgat caccccgcgg tgggcgacga gtcgtgagcg gatcggcatc gtcgtcggtt
     1381 ggctcatcgg cacggtcatc gtccaccaag ccttcgtcgc gctgtcctct tggccggcgc
     1441 agtggtggtt ctactggtgc gacgcgtgga tcttcgtcgg ctcggtgctc gccacctact
     1501 cgatggcccg tgggtggaac gagttctggc tgtgctggat cgccgtcgac ctcatcggcg
     1561 tcccgctcgg cttcgcgacc ggctacgtcc cgaccgccgt cctctacatc ggttacggcg
     1621 ccttcgtgat ctacggcttc acccagtggg tcaaggccac tcgcgaggag agcggctcgg
     1681 cacccgccga gtcgcctgcc gttcgctgag caaggtcact ggcttctgac cgggatcgcc
     1741 ggcagacgac gagaaggtcg tcgagcggtc ggatccgacc acaggtcgga tccggccgct
     1801 cggcgcatcg gggtgccgcc ctgctgtcgg cagcgcccgt cctgggtttc actgtgacgg
     1861 tggccacatt gcgatcgggc cgcggtgggc tggccggtca ccgcgacgcc ttcctcacct
     1921 ccggggacgc tggcggtggc gttcgcagcg tcgtcgccga ctcgtggatg cgctccagcg
     1981 ccagcggcgt agacccagac cggcacttgg ccccgatccg gatggatcga cctgaggtcc
     2041 agacctaccg atcggcccac ccgctcgccc aggtcttccc cttgctctac gacgtcctgg
     2101 gccgggctgc cgtcgactgt gattgcgtca tggccgtcgg ggacgccgac ggcacgctgc
     2161 tgtgggtgac cgggtcgccg acggtcatgc gccgagccga gaccatccac tttgtcgagg
     2221 gcgccgggtg ggacgagggc catgcaggca ccaatgcccc cggcatggcc ctgcatctgg
     2281 acgccgccac ccacgtgcgc tcagaggagc atttcaaccg cctggtgcag ccctggacct
     2341 gcgcggcagc accgatccac gatccgtcga cacagcagat cttgggcctg gtggacatca
     2401 ccggtggtcc ggacgtcgcc accccgcaga gcctggccct ggtgcgcgcc gcggcccgga
     2461 tggccgagtc ggagttggcc cggctgcagg cgacgggcgc gctgtggacc ccggccgctc
     2521 ccggacgccg cggctcgggt gacgcctcgc tgggccccga cccgtccgcc gccttcgccc
     2581 tgcgcgggct gggccagccc gaactcatcg ccgcccacgg cggacgcacc caccgcctga
     2641 gccggcgaca cggcgacatc ctcgtcgcgc tcgccgacca tccggatggc ctcactgccg
     2701 agcaactcga agttctcgtg tatgccgggc aggtcacctc atcgacgatg cgcgccgaga
     2761 tgacccggct tcgctcgatc ctcggtcccg aggtcctcac gtcgcgtccc tatcgcctca
     2821 tcgctggcgc ccagaccgac tggcacgcgg tcgcggcata cctggcggcc gggcggctgc
     2881 gcgccgcgat gcaggcctac cgcggtccgc tgctcccgat gtcgcagtcg cccgcagtcg
     2941 tcgatcagcg cgagattctc acgggacagg tccgcagtgc cctcatcgcc tcggccgagg
     3001 ccgatctcat ggtcgcctgg acccggtcgc gctggggggc cgacgacctg gacatgtggg
     3061 agcgtcaggc cgaaaccctg cccgctggct cgccgctgcg gccgatcgcg gtggccgaag
     3121 ccgcccgact cgagcgggag ttggtcgcag caccgatcgt gctggggcgc cagacccgcg
     3181 cctagccgga tcaggtgaga cctcgccaac gtgcatgcag cgttgttgca acgtcgcgct
     3241 gcatagcctc cggacacgag ccgtcgccgt gacggccgtc acagtcgcag agtgacctgc
     3301 gcaaccttcg gaggatgcac caatgacgat cttcgctccg cccggcagcg acggcagccc
     3361 ggtctccgtc gccagccgct acgacaactt catcggcggc cagtgggtgc ccccggtcaa
     3421 ggggcaatac ttcgagaacg tcaccccggt caccggtcgg gtcttcaccg agatcgcccg
     3481 cagcaccgcc gaggacatcg aactggcgct ggacgccgcg cacggcgccg cacccgcgtg
     3541 gggccgaacc tcggccgccg accgcgccgt cgtcctcaac aagatcgccg accggatcga
     3601 ggccaacctc gccgacctcg cgctggccga gacctgggac aacggcaaac cgatccgcga
     3661 gaccttggcg gccgacctgc ccctggccgt tgaccacttc cgctacttcg ccggtgccat
     3721 ccgggcgcag gaggggggca tctccgagat cgacgcagac accatcgcct accacttcca
     3781 cgagccgctg ggcgtcgtcg gccagattat cccctggaac ttcccgctgc tcatggcgac
     3841 gtggaagctc gcgccggccc tggcggcagg caacgcggtc gtcctcaagc cggccgagca
     3901 gaccccatgg tcgatcctca agctcatgga actcatcgct gacctgctgc ccgatggcgt
     3961 cctcaacgtc gtcaacggct tcggtgtcga ggccggcaag cccctcgcgt cctcgtcccg
     4021 catcgccaag atcgccttca ccggcgagac gacgaccggc cggctgatca tgcagtacgc
     4081 cagccaaaac ctcatcccgg tcaccctgga gctgggtggc aagagcccga acatcttctt
     4141 ctccgacgtt gccgacgagc gggatgcgtt ctacgacaag gcactcgagg gcttcgcgat
     4201 gttcgcgctc aaccagggcg aggtatgcac gtgcccgtcg cgggcgctga tccagtcgtc
     4261 gatctacgac aacttcctca tcgacgccgt cgagcgggtc ggcaagatca cccagggcaa
     4321 cccgctggac accgacacga tgatcggcgc ccaggcgagc aacgaccagc tcgagaagat
     4381 cctgtcctac atcg
//
LOCUS       DAOMZY010000295         4390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_18783_length_4390_cov_7.973241, whole genome shotgun sequence.
ACCESSION   DAOMZY010000295 DAOMZY010000000
VERSION     DAOMZY010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18783_length_4390_cov_7.973241"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..766
                     /locus_tag="PLK64_13930"
     CDS             131..766
                     /locus_tag="PLK64_13930"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPV80890.1"
                     /translation="MNLSSPMASVIPSVHGAVLAVLARTDQPLSGRGVAALTHGAASQ
                     RRVNDVLGVLVDAGIVLRERHPPAHLYRLNLAHVAAEGIMALSRQRELLLERIREEVA
                     SWDVAPVSASLFGSAARGEAGPHSDVDILVVASRHGPREAAIWDAQLDQLAQHVVDWS
                     GNHCEVLELTEAELADARVRNDRLVTELRTDAIALGGRDIRLLLTASQASA"
     gene            complement(1119..2528)
                     /locus_tag="PLK64_13935"
     CDS             complement(1119..2528)
                     /locus_tag="PLK64_13935"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HPV80891.1"
                     /translation="MGIFSRLFGGQRTGSGPTQGSPASAAGGRDQPRFAVLDVETTGL
                     NADRHRIIEIAVVTTDPWGRVLDQWTTRVNPQGPVGATHIHGITAEDVAGAPVFADVV
                     DALNGRLRGAAMAAHHARFDVAFLRAEYARAGWSLPHVPALCTLAASEVHLPSLERRR
                     LADCCTAIGHPLEGAHSALGDALGTAALLAAYMDPRRGVTPLQEHVDLPRAALMVAWP
                     SHPSSVLRSPHVSSTPSAGYQSRTRVVTPSTAPPAPLVQLVAHFSLVDALDEGAPSGS
                     LAYLEKLAVVLEDGLLTEDEISDLESVAVAEGLTPRDTAAAHHAFVLALAHEALRDGL
                     VSRSERAELHAVAALLEVPKASVTKVLDVAERSRNLRLSTGLRPLPSDWPHGEPLRVG
                     DKVAFTGCDDGVRAGLEARSEQLGVRIMSNVSTKTAMLVSDGTFEGTKAVKAQALGTR
                     IVDPTAYAVLLDHLQPALA"
     gene            complement(2562..>4390)
                     /locus_tag="PLK64_13940"
     CDS             complement(2562..>4390)
                     /locus_tag="PLK64_13940"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="damage-inducible protein"
                     /protein_id="HPV80892.1"
                     /translation="VGNPPYSVGQTSGNDNNANLHYPSLDARIEATYADRSSATNKNS
                     LYDSYIRAIRWASDRVGDAGVVGFVTNGGFVDSNTADGLRQSLIDEFSAVYIYNLRGN
                     QRTAGELSRREGGKVFGGGSRNTVAITFLVRTPGHSGPATLHYRDIGDYLTREDKLAI
                     VDGDHLASIDWQQVTPNAAGDWINQRGEEFDTFQPIGSKTPGAIFCVYSGGVKTNRDA
                     WVYNFDKTALTETVSRMVAFYNAEVARYEAAGAPKPPVDAFINTDPTKFSWNASDGKR
                     LVTGERYTAAPRRQRLSSYRPFARQWLWDDPRLLDRYYQLAQCFPTAAHENYGYVLTD
                     AGSHFEFCAIATDLLPNLHLLDTGKFFARYTYEPVDSGAAQGSLDFGHSDDVVIDGYR
                     RRDNITDETLTAYRGWYGDDATKDGIFAFVYGLLHSPDYRARFAADLKRSLPRIPRVE
                     AGDFAAFAEAGQRLLDLHIGYERAEPWPLTVTGDEPPAGEDPYAWFRVEKLKWGGSAR
                     DRDRSRIVYNARVAISGIPDEAHEYLLGSRTALEWVVDRYQVKTDKPSGIVNDPNDYA
                     REVGNPRYILDLIAKVTRVSVETVQIVNALPPLRIVDTERGR"
BASE COUNT          670 a         1465 c         1512 g          743 t
ORIGIN      
        1 ttacctcgag ctcaaggagt tccagcgcgt cgaggtcgtc gtcgcgccgt accagaggag
       61 ctgacaccca ccgtattgtg ctttttttcg ctcatctgag tgatgatgtg ctcattgagg
      121 tggtgagtcg atgaacctgt ccagccccat ggcgtcggtc atcccgagcg tgcacggcgc
      181 ggtgctcgcc gtgctggccc gaaccgatca gccgctgtct gggcgcggag tcgcggcgct
      241 cactcatggg gcagcgagcc agcgacgcgt caacgacgtc ctcggtgtac tcgtcgatgc
      301 tggcatcgtg ctccgcgagc ggcacccacc cgcgcacctg tatcgcttga acctggccca
      361 cgtcgctgcc gagggcatca tggcgctgag ccgccagcgg gagttgttgc tcgagcgcat
      421 ccgcgaagag gtcgcttcgt gggacgttgc accggtgtcg gcgagcctgt tcggctccgc
      481 ggcacgcggc gaggccggcc cgcacagtga cgtggacatc ctggttgtcg ccagcaggca
      541 cgggccacgt gaggcggcga tctgggatgc gcagctcgac cagctggcgc agcacgtcgt
      601 cgactggtca ggcaatcact gcgaggtcct cgaactgacc gaggccgaac tcgccgacgc
      661 tcgggtccgc aacgaccgtc tcgtcactga actgcgcacc gacgccatcg ccctgggcgg
      721 ccgcgacatc cggttgctgc tcacggcgtc gcaggcctcg gcatgacgac ccgcgacacg
      781 gcgcgcacgc acctgcgcaa ggcccgggag ttcctcgagg ctgccacgtc gcgctgaccc
      841 gcgggcgccc ttcggcctcc tgattgcgcc tgacgttgga tgccgcgccg gttgcctctc
      901 ggggcccgta cgattggggc gagtcctcag gaacctgccg cggatcgccc gcggtgctgc
      961 caccgctctc cttcagtgga tcggcccgcg gaggttcctg agtgcttcgg cgttcctcgt
     1021 cgcgggccgt tcagcactcg tcggacgcac gctgacgcag gtgtcgtcct tggcgcagcg
     1081 gagcgtggcg gcccctgagc gcgtcccgtc gccggaccct acgcgagggc gggttgcagg
     1141 tgatcaagca ggacggcgta ggccgtcgga tcgacgatgc gcgtgccgag cgcttgggcc
     1201 ttgacggcct tggtgccctc aaaagtgcca tcgctgacca acatggccgt cttcgtcgag
     1261 acgttggaca tgatccggac gccgagctgt tcactgcggg cctcaaggcc ggctcgcacc
     1321 ccgtcatcgc agcccgtgaa ggccaccttg tcgcccactc gcagcggctc cccgtgcggc
     1381 cagtccgacg gcaggggccg gagcccggtc gagagccgca gattgcggga tcgctcggca
     1441 acatcgagca ccttcgtgac actcgccttc gggacttcga ggagtgcggc aaccgcgtgc
     1501 agttcggcac gctcggagcg actgaccagc ccgtcccgca gggcttcatg cgcgagggcc
     1561 agcacaaacg cgtgatgtgc tgcagcggtg tcacgtgggg tcaggccctc cgcgaccgcg
     1621 actgactcga gatcggagat ctcatcctcc gtcagcaggc catcctccag caccacggca
     1681 agcttttcca ggtaagccaa gctaccgctc ggcgctccct cgtcgagggc gtcgacgagg
     1741 gagaagtggg cgacgagctg caccaagggg gcgggcggag cagtcgatgg ggtgacgact
     1801 cgcgtgcgcg actgataccc agctgacggt gtggaggaga cgtggggcga ccggaggacg
     1861 ctgctcggat gtgacggcca ggccaccatc agggcagccc gggggagatc cacatgctcc
     1921 tgaagcggag tgacgccccg gcgcggatcc atgtaggcgg ccagcagcgc tgcggtgccc
     1981 agcgcatcac ccaaggctga gtgggctccc tccagcgggt gcccgatggc cgtgcaacag
     2041 tcggccagtc gacggcgctc gagggacgga aggtgcacct cggatgccgc gagcgtgcac
     2101 aacgctggga cgtgaggcaa ggaccagcct gcccgcgcgt actccgcacg caggaaggca
     2161 acgtcgaatc gggcatggtg ggccgccatg gccgctcctc gcaggcggcc attcagcgca
     2221 tcgaccacgt cagcgaacac cggcgcaccg gcgacgtcct cagctgtgat cccgtggatg
     2281 tgggtggctc cgacgggtcc ttgtgggttg acccgggttg tccactgatc gagaacgcgg
     2341 ccccacgggt cggtggtcac gacggcgatc tcgatgatgc gatgacggtc agcgttcagg
     2401 cccgtggtct ccacatcgag caccgcaaac cgtggctggt cacgcccccc ggcggccgac
     2461 gcaggcgaac cctgcgtcgg cccggaaccg gtgcgctgac cgccgaacag tcgactgaag
     2521 atgcccatca gttctccctc accccagcgt ctgacggcag gtcatctgcc ccgctcagtg
     2581 tcaacgatcc tcaggggtgg gagggcgttg acgatctgga ccgtttcgac gctcacgcgg
     2641 gtgaccttgg cgatgaggtc gaggatgtag cgtgggttgc cgacctcgcg ggcgtagtcg
     2701 ttcgggtcgt tgacgatgcc cgacggcttg tcggtcttga cctggtagcg gtcgaccacc
     2761 cactccagcg ccgtccgcga gcccaacagg tactcgtgcg cctcatccgg gatgccgctg
     2821 atcgccaccc tcgcgttgta gacgatccgt gaccggtccc tgtcgcgggc actgccgccc
     2881 cacttgagct tctccacccg gaaccacgca tacgggtcct cgccggcggg cggttcgtcc
     2941 ccggtcacgg tcagcggcca cggctcggcg cgctcgtagc cgatgtgcag gtcgagcagc
     3001 cgctgaccgg cctcggcaaa cgcggcgaag tcacccgcct cgaccctcgg gatgcgcggc
     3061 agcgaccgct tgaggtccgc ggcgaaccga gcccggtagt cgggcgagtg cagcagcccg
     3121 tagacgaacg cgaagatgcc gtccttggtc gcgtcgtcgc cataccagcc ccggtatgcc
     3181 gtgagcgtct cgtcggtgat gttgtcccgc cgccggtaac cgtcgatgac cacgtcgtcg
     3241 ctgtggccga agtcgagcga cccctgcgcc gccccggagt cgacgggctc gtaggtgtac
     3301 cgcgcgaaga acttgcccgt gtcgagcagg tgcaggttgg gcagcaggtc cgtggcgatg
     3361 gcgcagaact cgaagtgaga cccggcgtct gtgagtacgt agccgtagtt ctcgtgcgcc
     3421 gcggtcggga agcactgagc aagttggtag tacctgtcaa ggagccgagg gtcatcccag
     3481 agccactgcc gagcgaacgg ccgatacgaa ctcaatcgtt ggcgacgagg agcagcggtg
     3541 tagcgctcac ccgtcaccag tcgctttcca tcagatgcgt tccacgagaa cttcgtcggg
     3601 tcggtgttga tgaacgcgtc gacgggtggc ttcggcgcac cggctgcctc gtagcgcgcg
     3661 acctcggcgt tgtagaacgc caccatgcgg ctcacggtct cggtcagcgc ggtcttgtcg
     3721 aagttgtaga cccacgcgtc ccggttggtc ttcaccccgc ccgagtagac gcagaagatc
     3781 gcgcccgggg tcttgctgcc gatgggctgg aaggtgtcga actcctcgcc gcgctggttg
     3841 atccagtctc ccgccgcgtt cggggtgacc tgctgccagt cgatgctcgc gaggtggtcg
     3901 ccgtcgacga tcgcgagctt gtcctcgcgg gtcaggtagt caccgatgtc gcggtagtgc
     3961 agcgtcgccg ggccgctatg accgggcgtg cgcacgagga aggtgatcgc caccgtgttc
     4021 cgggagccgc cgccgaacac cttgccgccc tccctgcgcg agagctcccc ggcggtgcgc
     4081 tggttgccgc gcaggttgta gatgtacacg gcggagaact cgtcgatgag gctctggcgc
     4141 agcccgtcgg cggtgttgga gtcgacgaag ccaccgttgg tcacgaaccc gacgaccccg
     4201 gcatccccga cccggtcgct cgcccagcgg atcgcgcgga tgtacgagtc gtagaggctg
     4261 ttcttgttcg tcgcgctcga ccggtcggca tacgtcgcct cgatccgggc gtccaggctc
     4321 gggtagtgca ggttggcgtt gttgtcgttg ccggaggtct gccccaccga gtacggcggg
     4381 ttgccgacga
//
LOCUS       DAOMZY010000296         4378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_18856_length_4378_cov_5.351608, whole genome shotgun sequence.
ACCESSION   DAOMZY010000296 DAOMZY010000000
VERSION     DAOMZY010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18856_length_4378_cov_5.351608"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1319
                     /locus_tag="PLK64_13945"
     CDS             <1..1319
                     /locus_tag="PLK64_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HPV80893.1"
                     /translation="FVMKDSYSFDLDEAGLDKSYQQHRDAYIKIFDRLGFHYVIVQAM
                     AGAMGGSKSEEFLATAENGEDTYVRCESCGYAANVEAVRVPAPAPIATEGMPSALVID
                     TPGTPTIESLVAFLNEAHPRADRAWTAADTLKNVVVLLSYPDGRTEPLVIGVPGDREV
                     DEKRLAAQIEPAVAVPFTDAEFAAHPGLVRGYIGPEALGADPERASATGIRYLTDPRV
                     SEGSAWVTGANLDGKHVIGLVAGRDFRSDGTIEAAEVKVGDVCPSCGQGDGLVAARGI
                     EMGHIFQLGTKYAKALDLKVLDESGKLVTVVMGSYGIGVSRAVACVAEGNHDQFGLVW
                     PREIAPADVHVVATGKDEEVFVAADTLARELAVQGLEVIYDDRRGVSPGVKFKDSELV
                     GVPTIVVVGRGLVDGKVEIKDRRSGERREVAVGEAVVEVCREVRGS"
     gene            1316..2107
                     /locus_tag="PLK64_13950"
     CDS             1316..2107
                     /locus_tag="PLK64_13950"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPV80894.1"
                     /translation="MTSGQMPGAVEAVIFDWGGTITPWHTVDLREQWHVFARGMGAIA
                     CSLNDTASAVLKAEDAAWRRGREEGTSARLDDILRTAGLDPDHPATHAGLAAYREFWE
                     PHTFTHPNIPEVWEWLRDNGIRVGVLSNTIWSRDYHREIFERDGVLHLVDADVYSSET
                     PWVKPRPEIFRAAAAAVGADPSACVYVGDRSYEDVHGPQSVGMRAIWIPHSDIPAEQQ
                     VSHEATPDAIAHELTDLVDIVSAWSFDGSSAGRSFGGPESPLGDA"
     gene            2100..2879
                     /locus_tag="PLK64_13955"
     CDS             2100..2879
                     /locus_tag="PLK64_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HPV80895.1"
                     /translation="MPDSPTVFALLVAAGLLAGWVDAVVGGGGLIQLPALLLGLPGAA
                     PAQLLATNKIASICGTSVSAATYYRRVRPDLRTALPMAGAAYVGAIVGALIGLHIPKS
                     AFNPVILALLVVVGAYTLLRPHLGTVTELRHSGTRHTSYAVLVGLVIGAYDGALGPGT
                     GSFLVFALVGWLGYAFLQASAKAKIANFATNLGALTVFVPQGQVLWVTGLAMGVANLV
                     GGYLGARTAVRLGSGFVRAVFMAVVGAFIVRLGGSVFGVWS"
     gene            3105..>4378
                     /locus_tag="PLK64_13960"
     CDS             3105..>4378
                     /locus_tag="PLK64_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HPV80896.1"
                     /translation="MSAATITPAHNGTAAGQAPARRPVGVPRTPQWWRDLAGTFTWLT
                     MLFVVALWVRNGGIQEFRSLAGGLSTLGRVTGMVSAQLLLIQVLLMARIPMVERSYGQ
                     DELARRHRLVGLWSFNLMWAHLELIWLGYAASSGAGLWGTIVDLVLNYPGMLLALAGM
                     VALCMVVVTSFRASRAALRYESWHLLHLYAYLGVGLALPHQLWTGQDFVGNLAATAFW
                     WTLWGAAIACTLTWRVGLPIWRTARHRLVVERVYPEGPGVTSVVVSGRDLDRIPVGAG
                     QFFQWRFLGNPGASRAHPYSLSAAPDGRSLRITAAHLGDGSAALAHLKPGSRVMIEGP
                     YGRLHGGVRRRRKVLLIASGIGVAPMRALLEELPQGPGDITLVYRAREQTDLIFTDEL
                     LALARARGARVFTVMGRRTTRRPSWLPESAAHL"
BASE COUNT          599 a         1501 c         1545 g          733 t
ORIGIN      
        1 agttcgtcat gaaggactcc tactccttcg acctcgacga ggccgggctg gacaagagtt
       61 accagcagca ccgcgacgcc tacatcaaga tcttcgaccg gctcggcttc cactacgtca
      121 tcgtccaggc gatggccggc gccatgggcg gcagcaagag cgaggagttc ctggccaccg
      181 cggagaacgg cgaggacacc tacgtccgct gcgagagctg cgggtatgcc gcgaacgtcg
      241 aggccgtgcg cgtccccgct cccgcgccga tcgcgacgga aggtatgccg tccgcgctcg
      301 tcatcgacac gcctggcacc ccgacgatcg agagcctggt ggccttcctc aacgaggcgc
      361 acccccgggc cgaccgcgcg tggaccgcag ccgacacgct caagaacgtc gtcgtgctgc
      421 tgagctaccc cgacgggcgc acggagccgc tggtcatcgg cgtccccggt gaccgcgagg
      481 tcgacgagaa gcgactggcc gctcagatcg agccggccgt cgccgtgccc ttcaccgacg
      541 ccgagtttgc ggcgcacccg ggtctggtgc gtggctacat cgggccggag gcgcttggcg
      601 ccgaccctga gcgggcgtcc gcgacgggga tccggtatct caccgacccc cgggtctccg
      661 agggcagtgc gtgggtgacc ggcgcgaacc tcgacggcaa gcacgtcatc gggctggtcg
      721 ccgggcgcga cttccgctcc gacggcacca tcgaggccgc cgaggtcaag gtcggcgacg
      781 tctgcccgtc gtgtggccag ggcgacggac tggtcgcggc gcgtggcatc gagatggggc
      841 acatcttcca actcggcacg aagtacgcca aggccctgga cctcaaggtc ctcgacgagt
      901 cgggcaagct cgtcaccgtc gtcatgggct cctatggcat cggggtctcc cgtgccgtcg
      961 cctgcgtcgc cgagggcaac cacgaccagt tcggtctggt ctggccgcgg gagatcgcgc
     1021 ccgccgatgt ccacgtcgtc gccaccggca aggacgagga ggtctttgtc gccgctgaca
     1081 cgttggctcg cgagttggca gtccagggcc tggaggtcat ctacgacgac cgccgcgggg
     1141 tctctccggg tgtgaagttc aaggactccg agctcgtcgg cgttccgacg atcgtcgtcg
     1201 tcgggcgcgg cctggtcgac gggaaggtcg agatcaagga ccgccggtcg ggggagcgac
     1261 gcgaggtcgc ggtcggcgag gccgtcgtcg aggtatgccg cgaggtccgc ggctcgtgac
     1321 gtccggccag atgcccggcg cggtcgaggc cgtcatcttc gactggggcg gcacgatcac
     1381 cccgtggcac accgtcgacc tgcgtgagca gtggcacgtc tttgctcgcg gaatgggtgc
     1441 gatcgcctgc tcgctcaacg acaccgcgtc ggccgtgctc aaggcggagg acgcggcgtg
     1501 gcgacgtggt cgagaggagg ggaccagcgc gcggctcgat gacatcctgc gcacggccgg
     1561 tctcgacccc gaccacccgg cgacgcacgc cgggctggcg gcataccggg agttctggga
     1621 gccgcacacg ttcactcacc cgaacattcc ggaagtctgg gagtggttgc gcgacaacgg
     1681 aatccgcgtt ggggtcctgt ccaacacgat ctggtcgcgc gactaccacc gcgagatctt
     1741 cgagcgcgac ggggtgcttc acctcgttga cgccgacgtc tactccagcg agaccccctg
     1801 ggtgaagccg cgaccggaga tcttccgtgc cgcggcggca gcggtcgggg ccgatccttc
     1861 ggcctgtgtc tatgtcggtg accgctccta tgaggacgtc cacgggcccc agtccgtcgg
     1921 gatgcgtgcg atctggatcc cgcactcgga catcccggcc gagcagcagg tctcgcacga
     1981 agccaccccc gatgcgatcg cgcacgagct gaccgacctg gtcgacatcg tgtccgcctg
     2041 gtccttcgat gggtcgtccg cgggcaggtc gttcggcggg ccggagtccc cgctcgggga
     2101 tgcctgacag cccaaccgtc ttcgcgctgc tcgtcgcggc tggcctgttg gccggctggg
     2161 tcgacgcggt ggtgggtggc ggcgggttga tccaactgcc cgcgttgctc ctcgggctcc
     2221 cgggcgccgc gccggcccaa ctgctggcca ccaacaagat cgcctcgatc tgcgggacct
     2281 cggtgagcgc ggcgacgtat taccggcgcg tgcggccgga cctgcgcacc gccctgccca
     2341 tggccggcgc cgcctacgtc ggtgccatcg ttggggcgct catcgggctg cacattccca
     2401 agagcgcgtt caacccggtc atcctcgcgc tgctcgtcgt cgtcggggcc tacacgctgt
     2461 tgcgtcccca cctcgggacg gtgaccgaac tgcggcactc cggcacgcgg catacctcgt
     2521 atgccgtcct cgtcggccta gtcatcggcg cctacgacgg ggcgctgggg ccggggacgg
     2581 ggtcgttcct ggtgtttgcg ttggtggggt ggctggggta cgcgttcctg caggccagcg
     2641 cgaaggccaa gatcgcgaac ttcgccacca atctcggggc gctgacggtg ttcgtgccgc
     2701 agggccaggt gctgtgggtg acgggactgg cgatgggtgt ggcgaacctg gtcggcggct
     2761 accttggtgc gcgcacggcc gtgcggctgg gaagtggttt cgtgcgtgcg gtgttcatgg
     2821 cggtggtcgg cgccttcatc gtgcgcctcg gtggctcggt cttcggggtc tggtcgtaga
     2881 aatgaccgtg cgagcgctat ctccggggag aatcggggga gcgggccgca aagatgaccg
     2941 tgcgagcgct atctccgtgg gaaaacgggg attgatcggg gggaggcgtg ggggtgtggg
     3001 tgcctggtga gagcatcgcg ggaggtgacg aacgcggcat acctcatgcc ccggaccgag
     3061 ccgactcaca ggaaactcct aggttcgccc ctcaggatcg acacatgtcc gcagcgacca
     3121 tcactcccgc gcacaacggg accgcggccg gccaagcgcc tgcccgccgc cccgtcggtg
     3181 ttccgcggac cccgcaatgg tggcgcgatc tggctgggac cttcacctgg ctcaccatgc
     3241 tgttcgtcgt cgccctctgg gtgcgcaacg gtgggatcca ggagttcagg tcgctggccg
     3301 gagggctgag cacgctcggc cgcgtgacgg ggatggtgtc ggcgcaactg ctgctcatcc
     3361 aggtgctgct catggcgcgc atcccgatgg tcgaacgctc ctacggacag gacgagttgg
     3421 ccaggcgtca ccggctggtc ggcctgtggt cgttcaacct catgtgggcc cacctcgagc
     3481 tcatctggct cgggtatgcc gcctcgtccg gagcgggcct ctgggggacg atcgttgacc
     3541 tcgtgctcaa ctacccggga atgcttctgg ccctggcggg gatggtcgcc ctgtgcatgg
     3601 tggtcgtcac gtcgttccgg gcgtcccgag cggcgctgcg ctatgagtca tggcatctgt
     3661 tgcacctcta cgcctacctc ggcgtggggc tggccctgcc gcaccaactc tggaccggtc
     3721 aggacttcgt cggcaacctc gctgcgaccg ccttctggtg gaccctctgg ggcgcggcga
     3781 tcgcctgcac gctcacctgg cgggtcggcc tgccgatctg gcggacggcg cgtcaccgac
     3841 tcgtcgtcga gcgggtctac cccgagggcc ccggcgtcac gtcggtggtc gtcagcggtc
     3901 gtgacctgga ccggatcccc gtcggcgccg ggcagttctt ccagtggcgg ttcctcggca
     3961 accccggtgc ctctcgcgcc cacccctact cgctgtccgc cgcacccgac ggccgctccc
     4021 tgcggatcac cgcggcgcac ctgggcgacg ggtcggccgc gctcgcccac ctcaagcccg
     4081 gctctcgggt gatgatcgaa ggcccctacg ggcgactgca cggcggggtg cgccggcgca
     4141 ggaaggtgct gctcatcgcc tccggcatcg gggtcgcgcc catgcgggcg ctcctggagg
     4201 agttgcccca aggcccgggg gacatcaccc tcgtctatcg ggctcgcgaa cagaccgacc
     4261 tcatcttcac cgacgaactt ctcgccttgg cccgagcccg cggtgcgcgc gtcttcacgg
     4321 tcatggggcg ccgcacgacc cgtcggccca gttggctccc cgagagcgcc gcccacct
//
LOCUS       DAOMZY010000297         4374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_18881_length_4374_cov_5.263950, whole genome shotgun sequence.
ACCESSION   DAOMZY010000297 DAOMZY010000000
VERSION     DAOMZY010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18881_length_4374_cov_5.263950"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PLK64_13965"
     CDS             <1..492
                     /locus_tag="PLK64_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV80897.1"
                     /translation="AGTLLMPRGLLVLDEPTNGLDPQGTREVRALIGSLGSEGTTVLV
                     SSHLLSEVAQVCSHLGVMREGRLVAQGPITELQGAATRATVRTDDGEQAAYVLRELGA
                     TGVSVDAEHAQVRGELGAIAPEKAVAALVHAGVAVRGFSSGAPDLEDWFVGLTGEGFD
                     VSG"
     gene            479..1417
                     /locus_tag="PLK64_13970"
     CDS             479..1417
                     /locus_tag="PLK64_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV80898.1"
                     /translation="MSAAEVSTAGAVTAGAVTAGAVTAGAVTAGATTAGVPSRPVPGW
                     AWRFFWSEVRLVAGRRRNQAGLAVLAAVPVVVAFAVKASAPRPGRGPDFLSSITSNGL
                     FVALTALTAEIAMFLPLAVAMLCGDAIAGEANQGTLRYLLTVPVTRTRLLFVKYASLV
                     FGALWGVTLVAVTGVLVGAALFGVGPLTTLSGSQLEFVPALGRLGLVVLYLTACLASL
                     AALGLFVSTLTEQPMAATITVMIVVILSWVLDGVPQVAWLHPWLIVHEWMSFGDLLRD
                     PVSLDGAGRGLWVALGYAVVFLAAAWARLSGKDITS"
     gene            complement(1612..3282)
                     /locus_tag="PLK64_13975"
     CDS             complement(1612..3282)
                     /locus_tag="PLK64_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HPV80899.1"
                     /translation="MKSTMQTTPLLISSMLSYGSTVHANAKVITWTGEGSRTTTFAEV
                     GTKSAQLAHALRGLGITGDQRVATFMWNNAEHLIAYLAVPSMGAVLHALNIRLFPPQI
                     IYVATHADNKVVIVDNTLAKPFSGLLPHLKTLRHVIVNGPVDAETWAALQAPEHIEGV
                     HDFNALIADQPTSFDWPEDIDENDASSMCYTSGTTGNPKGVAYSHRCNYLHATAVQNG
                     LSIRQGDHLLVVVPLFHANAWGFPYLAMISGATLVMPDRFLQAEPLAKMIEAEKVTGG
                     AGVPTIWNDLLQYLDKNPVDVSSCSMLMVGGSAAPPALIRSFKDKHNIDIVHGWGMTE
                     MSPVGSLAVPPVEVEVGSEAYWRYRNAQGRLLPGVEGRLLGPDDEVQPWDGESVGELE
                     VRGPWITASYYANGTESAAEQADMESKFHDGWLRTGDVGTLTADGFLVLTDRAKDVIK
                     SGGEWISSVDLENALMGHPDVREASVVGVPDEKWGERPLASVVLAEGSTTTVSDLREW
                     LGDKIPRWQVPERWAVIDEVPKTSVGKFDKKVLRARYAEGGMEVTTLG"
     gene            3423..4301
                     /locus_tag="PLK64_13980"
     CDS             3423..4301
                     /locus_tag="PLK64_13980"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097159 - organic cyclic compound binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5-like heme/steroid binding
                     domain-containing protein"
                     /protein_id="HPV80900.1"
                     /translation="MLTVALSTESGPFDTIAGLPVHPLVVHGAVVLLPLAALGVLALV
                     LVRRWRGVFGWLTLAGLAAGAGAAIVAEESGEALAARVGMPAEHAEWGERLPPVAVAL
                     FIVTLVWFLVARRAARRQGAGGAPQGASAMVTVGGILAAVLAVATIAVTVLVGHSGAT
                     AVWSGKIVATSASATTAATPSATSATTPSTSSSSSSSSSSSSSSSSSTTSGSGAAAYA
                     MTQVATHNTSASCWAAVSGSVYDLTAWIGEHPGGRDRIIGLCGTDATAAFAAQHLGQG
                     EPAEELTRFKIGTLAG"
BASE COUNT          600 a         1406 c         1588 g          780 t
ORIGIN      
        1 gcgggcaccc tgctcatgcc gcgcggcctc ctcgtcctcg acgaacccac caacgggctg
       61 gacccccaag gcacccgtga ggtgcgggcc ctcatcgggt cgctcggcag cgaaggtacg
      121 acggtgctcg tctccagcca cctgctctca gaggtcgccc aggtctgctc gcatctcggt
      181 gtgatgcgcg aaggccggct ggtcgcccaa ggcccgatca ccgagttgca gggcgcggcc
      241 acccgggcga cggtgcggac cgacgacggt gagcaggcgg catacgtgct gcgtgagctc
      301 ggcgcgacgg gggtgagcgt cgatgcggag catgctcagg tgcgaggtga gctcggcgcg
      361 atcgccccgg agaaggcggt agcggcgctc gtccacgcgg gggtcgcggt ccgcggattc
      421 tccagtgggg caccggatct cgaggattgg ttcgttggcc tgacagggga gggttttgat
      481 gtcagcggct gaggtgtcga cggctggcgc ggtgacggct ggcgcggtga ctgctggtgc
      541 ggtgactgct ggtgcggtga ctgctggcgc tacgacggct ggcgtgccgt cgcggccggt
      601 gccgggctgg gcctggcggt tcttctggtc ggaggtgcgc cttgtcgcgg ggcgtcggcg
      661 caatcaggcg ggcctggcgg tgttggccgc cgtcccggtc gtcgtggcgt tcgcggtgaa
      721 ggcctcggca ccccggccgg gtcgtggccc ggacttcctg tcgagcatca cctccaacgg
      781 actgttcgtc gcgctcacgg cgttgaccgc ggagatcgca atgttcctgc cgcttgcggt
      841 ggcgatgttg tgcggtgacg ccatcgcggg cgaggccaac caggggacat tgcgctatct
      901 gctcaccgtg cccgtcacgc gcacccggtt gctgttcgtc aagtacgcct cgctcgtttt
      961 cggcgcgctt tggggggtca ctctcgtggc ggtcaccggg gtgctcgtgg gcgcggcgct
     1021 gttcggggtc gggccgctga cgaccctctc gggcagccaa ctggagttcg tcccggctct
     1081 cggtcgactc gggctggtcg tcctctatct cacggcctgc ctcgcgtcgt tggcggcgct
     1141 ggggctgttc gtgtcgacgc tcaccgagca gccgatggcc gcgacgatca cggtgatgat
     1201 cgtcgtcatc ctctcgtggg tcctcgatgg tgtcccgcag gtggcgtggc tgcacccgtg
     1261 gttgatcgtc cacgaatgga tgtccttcgg agacctgctg cgcgatccag tctcgctcga
     1321 cggtgccggc cgcgggctct gggtcgcgct cggttatgcc gtcgtcttcc tcgccgctgc
     1381 ctgggcccgg ctgtcgggca aggacatcac cagctgagcc agtgccgaca ccggcggtgg
     1441 ggaggggcca ggggggctga gatttccccg tacttcaggt acgcagtgcg tacctgaagt
     1501 acgtcgtttg aatagccccc ctgggaggtg gacaagcggg ctggcgagca gacggcccgg
     1561 tgcgcagagg gccgaagtga gcacccggca taccgggaag gcctgaggca atcagcccag
     1621 ggtggtgacc tccattccgc cttcggcgta acgagcgcgc aagaccttct tgtcgaactt
     1681 gccgacgctc gtcttgggca cctcgtcgat gacggcccag cgctcgggca cctgccagcg
     1741 cgggatcttg tcgccgagcc actcgcgcag gtcggacacg gtcgtcgtgc tgccctcggc
     1801 gagcacgacg ctggccagcg gacgctcacc ccacttctca tcggggacac cgacgacgga
     1861 cgcctcgcgg acatcggggt ggcccatgag ggcgttctct aggtcgacgg aggagatcca
     1921 ctcgccgccg gacttgatga cgtccttcgc gcggtcggtg aggacgagga agccgtcggc
     1981 ggtcagggtg ccgacgtcgc cggtgcgcag ccagccgtca tggaacttcg actccatgtc
     2041 ggcctgctcc gccgccgact cggtgccatt ggcgtaatag gaggcggtga tccacggacc
     2101 gcggacctcg agctcgccga cgctctcgcc gtcccacggc tgcacctcgt cgtccggccc
     2161 gagcaggcgg ccttcgacgc cgggcagcag gcgcccttgg gcattgcggt agcgccagta
     2221 ggcctcggag ccgacctcga cctcgacggg cggaaccgcc agcgagccga cgggcgacat
     2281 ctcggtcatg ccccagccgt ggacgatgtc gatgttgtgc ttgtccttga acgagcgaat
     2341 gagcgccggc ggggcggcgg atccgccgac catgagcatc gagcacgacg agacgtcgac
     2401 ggggttcttg tcgagatact gcagcaggtc gttccagatc gttgggacgc ctgcgccacc
     2461 ggtgaccttt tcggcctcga tcatcttcgc cagcggctcg gcctgcagga accggtcggg
     2521 catgaccagc gtcgcgccgg agatcatcgc gaggtagggg aacccccacg cattggcgtg
     2581 gaagagcggg acgacgacga gcaggtggtc gccctggcgg atcgagagcc cgttctgcac
     2641 agccgtggcg tggaggtagt tgcagcggtg cgaataggcg acgcccttgg ggtttccggt
     2701 cgttccggag gtgtaacaca tcgaggacgc atcgttctca tcgatgtctt cgggccagtc
     2761 gaagctggtg ggctggtcgg cgatgagggc gttgaagtcg tgcacgccct cgatgtgctc
     2821 gggggcctgc agtgccgccc aggtctcggc gtcgactgga ccgttgacga tgacgtgacg
     2881 gagggtcttg aggtggggga gcagcccgct gaacggtttg gccagcgtgt tgtcgacgat
     2941 gaccactttg ttgtcggcat gcgtcgcgac ataaatgatc tgcggcggga agaggcgaat
     3001 gttgagcgca tgcaggacag cgcccatcga ggggaccgcg agataggcga tcaggtgctc
     3061 ggcgttgttc cacatgaacg tcgccacccg ctggtcgccg gtgatgccca agcctcgcag
     3121 ggcgtgcgcg agctgcgccg acttggtgcc gacctcggcg aatgtcgtgg tgcgagaacc
     3181 ctcccccgtc cacgtgatga ccttggcgtt ggcgtggacc gtggagccgt agctcagcat
     3241 cgaggagatg agcagtggcg tcgtctgcat ggtgctcttc atgcccctca gcctgccagg
     3301 gtcaccctcg tttgacgaga gccaggcggt cgtccgcgcc catgaaattg gacatatggg
     3361 taggtgccac tgcgaccaaa gcaggctgtg cgctcacaga aggtgaaggt acgttgacac
     3421 acatgctgac cgttgcactg tcaaccgaat cgggcccgtt cgacacgatc gccggcctgc
     3481 cggtccatcc gttggtcgtt cacggagccg tcgtcctgct tcccctggcg gccctagggg
     3541 tcctcgctct cgtgctcgtg cggcgctggc gcggggtctt cggctggctg acgctcgccg
     3601 gtctggcggc aggtgctgga gcggccatcg tcgcggaaga gagcggcgag gcactcgccg
     3661 cacgcgtcgg tatgccggcc gagcacgccg agtggggcga gcggctgccg cccgttgccg
     3721 ttgcgctctt catcgtcacc ctcgtgtggt tcctcgtggc gcgtcgggct gctcggcgcc
     3781 agggtgcagg cggggctccc cagggcgcgt ccgcgatggt gaccgtgggc ggcatactcg
     3841 ctgcggtgtt ggccgtggcg acgatcgcgg tcacggtcct cgtcggacac agcggcgcca
     3901 cggccgtgtg gagcggaaag atcgtcgcga cgtcggcgtc ggcaacgacg gcagccactc
     3961 cgtcggcgac gtcggccact accccgtcga ccagctcgag ctcgtcgtct tcgtcctcgt
     4021 cgtcctcgtc ttcgtcgtcc tcgacgacgt ccggcagcgg cgcggcggca tacgccatga
     4081 cgcaggtggc cacccacaac acgtcggcct cgtgctgggc cgcagtcagc ggttcggtct
     4141 atgacctcac tgcctggatc ggcgagcacc ccggcggccg ggaccggatc atcggtctgt
     4201 gcggcaccga cgcgaccgcg gctttcgctg cgcaacacct cgggcaaggc gagccggccg
     4261 aggaactgac gcgcttcaag atcgggacgc ttgccggctg acggcggagg tccgctgctg
     4321 cgtcgacgtc gccccggatc ccggcggcga cggcgtggcg gcggcatacc tctg
//
LOCUS       DAOMZY010000298         4356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_18985_length_4356_cov_5.890026, whole genome shotgun sequence.
ACCESSION   DAOMZY010000298 DAOMZY010000000
VERSION     DAOMZY010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18985_length_4356_cov_5.890026"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PLK64_13985"
     CDS             complement(<1..675)
                     /locus_tag="PLK64_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPV80901.1"
                     /translation="MTTKAPTVTGEPTETTFTVGQVADLLGVTVRTLHHYDGIGLVVP
                     SERSRAGYRLYTTADLTRLQHVVVYRRLEFPLEQIATLLEEGATDPRASAEHLRRQRA
                     AVLTRLDELRDLVTAIDTALEATMNDRPATREEMRDLFGDGFSDDYQAEAQERWGESA
                     AWKQSAARTARYTKADWVEVKAEMAAVGEGFAAAMDAGMPPTSEAAMDAAEAHRASIE
                     RFYDCSS"
     gene            complement(770..>1246)
                     /locus_tag="PLK64_13990"
     CDS             complement(770..>1246)
                     /locus_tag="PLK64_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPV80902.1"
                     /translation="DVVLMDLSMPGIDGIEGTARLRDSGEETRVVVLTSFVEHERVTA
                     ALAAGAVGYLLKDSDPRDVLEAIRSAAAGYAPLDARVTRALLPSAKPVDPAAALSVRE
                     REVLVLVAQGMANKQVARALGISERTVKAHLGNVFRHIGVADRTSAALWARDHNVG"
     assembly_gap    1247..1346
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1347..1701)
                     /locus_tag="PLK64_13995"
     CDS             complement(<1347..1701)
                     /locus_tag="PLK64_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPV80903.1"
                     /translation="MIATPVSPPAESPLDTTPEPSVVAADDRVELAPAPASTAAPTTG
                     GTTGGATRATTVVLVDDHRLVRAGLRGIIDAAPDLTVVGEAADGAEALAVVRATQPDV
                     VLMDLSMPGIDGIEGT"
     gene            complement(1698..3101)
                     /locus_tag="PLK64_14000"
     CDS             complement(1698..3101)
                     /locus_tag="PLK64_14000"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPV80904.1"
                     /translation="MGAEGTSSPAPAAGWVTLADGQPGERAQALPTPGRTLAQLVIAA
                     LIVLGLVLVGTSFAASRLAEREAVNDAAHTANLLAQAVIQPALTDALVAGDPAAFAAF
                     DRVVRDSVLPNNIVRVKLWHPDGTIVYADETRLVGQKFTLDPEQLEVLTVPQTRAEVS
                     ELDRSENEFERYGGKLLEVYRPVWTPGGEALLFEVYGAYAPVAERTSDLWRGLAGLIA
                     SSLLLLFVLMTPILWKVLDRLGQAHAHREDLLRRAVDASDEERRRIAATLHDGPVQEL
                     VASSLVVAGAAEQASSAGHAQLADDIREAGGTVRASIASLRSLLVDLYPDRLADAGLA
                     AAVDDLGGPLRSRGTTVTIDLDPVAVAQLDEESTRLIYRVTRECLRNVAKHAGACTVS
                     VTLAPAGQGRHTELLIADNGVGFDVAASRARHGHFGLRVLTDLAEGAGAVLQVASAPG
                     AGTRWRLLLPDQPGGMP"
     gene            3283..3726
                     /locus_tag="PLK64_14005"
     CDS             3283..3726
                     /locus_tag="PLK64_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80905.1"
                     /translation="MSSELEPELSSESSESSEVKEDELVEALEDDRDEVLDDDPVVLE
                     DEALVLDEVDVRGRSPPWSSPGSAVRVVRSPPLSSAVPAGVLDELLTSCTEAGTAVGN
                     DWANDSLPSNPRAAGTTAAVAAIAITMRFMVGSLQVVGRGGPATT"
     gene            complement(3814..4308)
                     /locus_tag="PLK64_14010"
     CDS             complement(3814..4308)
                     /locus_tag="PLK64_14010"
                     /inference="COORDINATES: protein motif:HMM:NF039475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6508 domain-containing protein"
                     /protein_id="HPV80906.1"
                     /translation="MARDLRPKDLRSLDPLTTDQVTQAARLHACLQAHHGEWGHWEGG
                     KVGADGAQTPHRVILTPIAQETYDWLLTSGVMPDFNWLAWPAKRELVQGNVSTLEPDV
                     ARALLTALIVQEHSSDGSLLGIFRHGIITNLLGVLGTSPHHPELEASPDPVDDGHPLV
                     LEPI"
BASE COUNT          620 a         1453 c         1530 g          653 t
ORIGIN      
        1 cgacgaacag tcgtagaagc gctcgatcga cgcccgatgc gcctcggcgg cgtccatcgc
       61 agcctcgctg gtgggcggca taccggcgtc catagccgcc gcgaaaccct cgccgacggc
      121 cgccatctct gccttgacct cgacccagtc ggccttggtg tagcgcgcgg tccgggccgc
      181 ggactgcttc cacgccgccg actcacccca acgttcctgc gcctcggcct ggtagtcgtc
      241 ggagaacccg tcgccgaaca ggtctctcat ctcctcgcgg gttgcgggtc ggtcgttcat
      301 cgttgcctcc agagcggtgt cgatggccgt gacgaggtcc cgcaactcgt ccagccgcgt
      361 caggaccgcg gcccgttgcc gacgcaagtg ctcggcgctg gcccgcggat cggtggcgcc
      421 ctcctcgagc aaggtggcga tctgctcgag cgggaactcc aaccggcggt agacgacgac
      481 gtgctgcaag cgggtgaggt cagcagtggt gtagagccga tagcccgcgc gcgagcgctc
      541 gctcggcacg accaacccga tcccgtcgta gtggtgcagc gtccgcaccg tcaccccgag
      601 caggtcggcc acctggccca ccgtgaaggt ggtctcggtg ggttcgcccg tcacagtggg
      661 tgccttcgtc gtcatgcgaa ccagcgtcgc ggctcacgtc gggtgagggt caagtccgtt
      721 ccccgcactg tatgccgccc gcctcgcctt ggtgcctcac gcaggcgtgc tagccgacgt
      781 tgtggtcgcg ggcccacagc gcggcgctgg tgcgatcggc cacgccgatg tgccggaaga
      841 cgttgcccag gtgggccttc acggtgcgtt cggagatccc cagggctcga gcgacctgct
      901 tgttggccat gccctgggcg acgagcacca acacctcccg ctcccgcacg ctcagcgccg
      961 cggccggatc aaccggcttg gcgctcggca gcagcgcccg cgtcactcgg gcatccaggg
     1021 gggcgtaccc ggcggcggcg gagcggatcg cttcgaggac gtcgcgaggg tcgctgtcct
     1081 tgagcagata gccgacggcc ccggccgcca acgctgcggt cacccgctca tgctcgacga
     1141 acgacgtgag cacgacaacc ctggtctcct caccggagtc ccgcaggcgg gcggtccctt
     1201 cgatcccgtc gatgcccggc atcgacaggt ccatgaggac gacgtcnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnncggt cccttcgatc ccgtcgatgc ccggcatcga
     1381 caggtccatg aggacgacgt cgggctgggt ggctcgcaca acggcgaggg cctccgcacc
     1441 gtcggcggcc tccccgacga cagtcaggtc gggggccgcg tcgatgatgc cgcgcaaccc
     1501 agcgcggacc aatcggtggt catcgacgag gacgacggtt gtcgcccggg tcgccccgcc
     1561 ggtcgtccct ccggtcgtcg gcgcggccgt tgacgcgggc gcgggcgcga gctcaacccg
     1621 gtcgtcggcc gcaaccaccg acggctcggg ggtggtgtcg agcggcgact cggcgggcgg
     1681 ggacacggga gtggcgatca cggcatacct ccaggctggt cgggcaggag caggcgccaa
     1741 cgtgtgcctg ctcccggggc cgaggcgact tggagcacgg ctccggcgcc ttcggcgagg
     1801 tcggtgagca cccgcaggcc gaagtggcca tgtcgggcgc ggcttgctgc gacgtcgaac
     1861 ccgacgccgt tgtcagcgat gagcaactcg gtatgccgcc cttgccccgc aggcgcgagc
     1921 gtcaccgaga cggtgcaggc accggcgtgc ttggcgacgt tacgcaggca ctcccgggtc
     1981 acgcggtaga tcaaccgggt cgactcctcg tcgagctggg ccacggcgac cgggtccaga
     2041 tcgatggtca ccgtggtgcc acgcgagcgc agcggacccc cgaggtcgtc cacggcggcg
     2101 gccagtcccg cgtcggcgag ccggtccgga tacaggtcga ccaacagcga gcgcaggctg
     2161 gcgatgctcg ctcgaaccgt tccgccggct tctcggatgt cgtcggcgag ctgggcgtgg
     2221 ccggccgagc tggcttgttc ggcagctcca gccacgacaa gcgagctggc gacgagctcc
     2281 tggaccgggc cgtcgtgcag ggtcgccgcg atccggcggc gctcctcgtc ggaggcgtcg
     2341 acggcgcggc gcagcaggtc ttcgcggtgg gcgtgggcct gtccgagccg gtcgaggacc
     2401 ttccagagga tcggggtcat gaggacgaag agcagcagca agctgctcgc aatgagcccg
     2461 gccaaccccc gccagaggtc gctcgtccgt tcagcgaccg gggcgtaggc gccgtagacc
     2521 tcgaagagca acgcctcacc gcccggggtc cagaccggcc gatagacctc aagcagcttg
     2581 ccgccgtagc gctcgaactc gttctccgaa cgatccagct cggagacctc ggcccgggtc
     2641 tgcgggacgg tcaagacttc gagctgctcg gggtcgaggg tgaacttctg accgaccagc
     2701 ctggtctcat cggcatagac gatggtgccg tccgggtgcc acagcttgac tctgacgatg
     2761 ttgttgggca ggacgctgtc cctgacgact cggtcgaagg cggcgaacgc ggccgggtcg
     2821 ccggccacga gggcgtcggt gagggccggc tggatgaccg cctgcgccag cagattcgcg
     2881 gtgtgcgccg cgtcgttgac cgcctcgcgc tcagccaggc ggctggccgc gaaggacgtg
     2941 ccgaccagga ccaggcccaa cacgatcaac gcagcgatga cgagttgggc gagcgtgcgt
     3001 cctggggtgg gcaaggcctg ggcgcgctcg ccgggttgtc cgtcggcgag ggtgacccag
     3061 cccgcggcgg gcgccgggga cgaagtgccc tcggcgccca cccatggtgt cgatgacgcg
     3121 gtcagcgttc gcacctttca gtcgtcggag ccgtggccac cggagccgct gtcgtcaccg
     3181 gagtcgtggc cagaaccatc atcgctgccg ccgcccgagg aacccgaacc cgagcccgag
     3241 ttgtcgtccg agccggagcc agagcccgag ccagagcccg agttgtcgtc cgagctggag
     3301 cccgagttgt cgtcggagtc gtccgagtcg tcggaggtga aggaggacga gctggtcgag
     3361 gcgctcgagg atgaccggga cgaggtgctc gacgacgatc cggtcgtgct cgaggacgag
     3421 gcgctcgtgc tcgatgaggt ggacgtgcgc ggccggtcgc cgccgtggtc gtctcccggc
     3481 tcggcggtgc gggtggtccg gtcgccgccg ttgtcgtcag cggtccccgc tggagtgctc
     3541 gacgagctgc tgacgagttg cactgaggcc gggactgcgg tgggcaacga ctgggcgaac
     3601 gattcgttgc cgagcaatcc cagggctgcg ggcacgactg ccgccgttgc tgcgatcgcc
     3661 ataacgatgc gcttcatggt gggttccctt caggtggtgg gccggggtgg tccggccacg
     3721 acctaaaggt gcgccaggag cgcgggcagc cgccatcggg ctttggtcgt agtacccacg
     3781 caggggtatg ccgccaccca gccgtctgct gggtcagatg ggttccagga ccagcgggtg
     3841 gccgtcgtca accgggtctg ggctcgcctc aagctcaggg tggtgcggac tggtgccgag
     3901 aaccccgagc aggttggtga tgatcccgtg ccggaagatc cccagcagcg acccgtcgga
     3961 cgagtgctcc tgcacgatga gggccgtgag caacgcccgg gcgacgtcgg gctccaacgt
     4021 cgacacgttg ccctggacca actcacgctt ggccggccac gccagccagt tgaagtccgg
     4081 catgacccct gaggtcagca gccagtcgta ggtctcctgc gcaatcggcg tgaggatgac
     4141 gcgatgaggg gtctgcgccc cgtcggctcc gaccttgccg ccctcccagt gcccccactc
     4201 accgtggtgg gcctgcaggc aggcgtgcaa ccgagcggcc tgggtgacct gatcagtggt
     4261 cagggggtcc agagatcgca ggtccttcgg tcgcagatca cgcgccatgg ttcctcctca
     4321 ctcgcgctcg gtccaccgcc tcacggaggt gcgaac
//
LOCUS       DAOMZY010000299         4351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_19024_length_4351_cov_4.813780, whole genome shotgun sequence.
ACCESSION   DAOMZY010000299 DAOMZY010000000
VERSION     DAOMZY010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19024_length_4351_cov_4.813780"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /gene="glgA"
                     /locus_tag="PLK64_14015"
     CDS             <1..1060
                     /gene="glgA"
                     /locus_tag="PLK64_14015"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HPV80907.1"
                     /translation="DEAGTTAYPDLPELASANAALQTFGVDVTIAQDCQGADIVHSHT
                     WYANLGGHLGGLLAGAPHVITAHSLEPMRPWKAEQLGGGYRVSSFAERTAYEGAAAVI
                     AVSAGMRADILRSYPSLDPAKVPVVHNGIDSQMWQPIHDADVCRKHGMDPDRPAVTFV
                     GRITRQKGLPYLMRAAARLPEEVQVVLCAGAPDTPEIKAEVEGLLADLRAQRDGVVWI
                     PEMLPRSEVIALLTASTVFACPSVYEPLGIVNLEAMACELPVVATATGGIPEVVVPGE
                     TGWLVPIEQVQDGTGTPVDPEKFVGDLADALTNAVSDRERARTMGLAGRKRAVEQFSW
                     ESIGDKTMAVYQDVLGRS"
     assembly_gap    1122..1221
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1222..1739)
                     /locus_tag="PLK64_14020"
     CDS             complement(<1222..1739)
                     /locus_tag="PLK64_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80908.1"
                     /translation="MLALPIVVRCALWGTRWLGGRGDLDDVVGNVHSGAVTDIAGPFE
                     ATLRDWAERGETAVFLALPRPGLLTGMPRAPLAVTAAATDAGQAAYAPTVGGALVPAL
                     TRFGPEGDTGVLATWAAYETEPVPRHIAEAPDVGDLSRRLTEAVQEATTRLATVGGIP
                     WRGGEATPPPRA"
     gene            1878..>2356
                     /locus_tag="PLK64_14025"
     CDS             1878..>2356
                     /locus_tag="PLK64_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPV80909.1"
                     /translation="MSDVLAFAGVSVVRGHNRLLDDVTWEVEEGERWVILGPNGAGKT
                     TLLQIAAARMHPTTGVVGILGEVLGTVDVFELRPRVGLASASIAERIPGGESVGNVVV
                     TASYGIIGRWRESYDRQDYARAMGLLEALGVAHLADRLYGTLSEGERKRVQIARALMT
                     "
     assembly_gap    2357..2456
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2631..3092
                     /locus_tag="PLK64_14030"
     CDS             2631..3092
                     /locus_tag="PLK64_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPV80910.1"
                     /translation="MFQGSSWLIWVGVALALGAIEAATVDFIFLMLAGGALAGAVAAA
                     FGADFPGQAIVASVSAVALLVGVRPWVKRRFNVKTERPTVGIAGNVGRTATVLETVTL
                     AGGRINLSGETWSARSQGGDAILPGDKVEVIAIDGATAVVAPVTLPPQPLP"
     gene            3165..4229
                     /locus_tag="PLK64_14035"
     CDS             3165..4229
                     /locus_tag="PLK64_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPV80911.1"
                     /translation="MPRIETTIVLLLLLVFAITVVVRTIRIVPQQTAVIIERLGSYSR
                     TLDDGMHVLVPFIDKPRATIDLREQVVTFPPQPVITSDNLVVSIDTVIYYTVTDAKSA
                     VYEIANFIQGIEQLTVTTLRNVIGSLDLEETLTSRDQINGQLRGVLDQETGRWGIRVN
                     RVELKAIDPPHSVQDSMEKQMRAERDKRAAVLTAEGVKQSAILTAQGEKEAQILRAEG
                     SAQARVLEAQGQAQAIQQVFDAIHRGKPTQRLLAYQYLQVLPQLARGDANKMWIIPSE
                     LTDALRGVSSALGGNSSGAALEEEWVEPAPAPAAAPPIQRAKQFGEPEIVGDVHPPTH
                     PVTQPVAPPPPPPRTPDSAQ"
BASE COUNT          617 a         1449 c         1417 g          668 t
ORIGIN      
        1 cgacgaagcc ggcaccacgg cataccccga tctgcccgaa ttggcctctg ccaacgcggc
       61 attgcagacg ttcggggttg acgtcaccat tgcccaggac tgtcagggcg cagacatcgt
      121 tcactcgcac acctggtacg ccaacctggg cgggcacctc ggtggcctgc tcgccggagc
      181 cccgcacgtc atcacggccc acagccttga gccgatgcgc ccgtggaagg ccgagcagct
      241 cggcgggggc taccgcgtga gctcgttcgc cgagcgcacg gcctacgaag gggctgcggc
      301 ggtcatcgcc gtgagcgccg ggatgcgcgc cgacatcttg cgcagctatc cgtcgctcga
      361 cccggccaag gtgccagttg tgcacaacgg gatcgactcg cagatgtggc agccgatcca
      421 cgacgccgac gtctgccgca agcacggcat ggacccggac cggcccgcag tgaccttcgt
      481 cggccggatc acccggcaga agggcctgcc ctatctcatg cgggctgccg cgcgtctgcc
      541 cgaggaggtc caggtcgtcc tctgcgcagg cgcccccgac acgcccgaga tcaaggccga
      601 ggtcgagggt ctgctggctg acctgcgcgc ccagcgcgac ggcgtcgtct ggatccccga
      661 gatgctgccg cgcagtgagg tcatcgccct gctcacggcg tcgacggtct tcgcctgccc
      721 gtcggtctac gagcccctcg gcatcgtcaa ccttgaggcg atggcctgcg agttgcccgt
      781 cgtcgcgacg gcaaccggcg gcattcccga ggtcgtcgtc cccggcgaga ccggctggct
      841 cgtgccgatc gagcaggtcc aggacggcac cgggacgccc gtcgacccag agaagttcgt
      901 cggcgacctc gccgatgctc tcaccaacgc ggtgagcgac cgcgagcggg ctcggacgat
      961 ggggttggcc ggccgcaagc gtgcggtcga gcagttctca tgggagtcga tcggcgacaa
     1021 gacgatggcc gtctatcagg acgtcctcgg tcgctcgtga tctcgctcgt gatctcaagg
     1081 gggcgcctcg gccgctcgtg atgcgccgcc gaggcccctc gnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nctcgcccgc ggcggtggcg tcgcctcacc accgcgccac
     1261 ggtatgccgc ccaccgtcgc cagccgggtg gtcgcctcct gcaccgcttc ggtcagtcgt
     1321 cggctgagat cacccacatc cggggcctcg gcgatgtggc gggggaccgg ctcggtctcg
     1381 tatgccgccc acgtcgccag cacgccggtg tcgccttccg ggccgaagcg ggtcagcgcc
     1441 ggcacgagcg ccccaccgac cgtcggcgcg tatgccgcct ggccggcgtc cgtcgccgcc
     1501 gcggtcacgg caaggggggc acgcggcata cccgtgagaa gtcctgggcg ggggagggcg
     1561 aggaagacgg ccgtctcgcc gcgctcggcc cagtcgcgca gggtcgcctc gaagggcccg
     1621 gcgatgtcgg tgacggcgcc tgagtgaacg ttgccgacga catcgtcgag gtcgcctcga
     1681 ccgcccagcc atcgggtgcc ccagagggca caccggacga cgatcgggag cgcgagcacc
     1741 ggcacaccat aaccatcgac tcggccgaac gcccgcctcc gccggcgggt ttggacctgt
     1801 ggttgaagtg gatgggggtg ccgacacgct cccttggcgc ggggccgcga actcctcgcg
     1861 ggctagggtg ggtgaccatg agtgatgtgc tcgccttcgc gggcgtcagc gtggtgcgcg
     1921 gccataaccg cctcctcgac gacgtcacct gggaggtcga ggaaggcgaa cgctgggtca
     1981 tcctcggccc caacggggcc ggcaagacca ctctgttgca gatcgccgcc gcgcgcatgc
     2041 accccaccac cggtgtcgtc gggatcctcg gcgaggtgtt gggcaccgtt gatgtcttcg
     2101 agttgcggcc gcgggtcggt ctggccagtg cctcgatcgc cgagcggatc cccggcggtg
     2161 agagcgtcgg caacgtcgtc gtcaccgcgt cctacgggat catcgggcgc tggcgtgaaa
     2221 gctatgaccg ccaggactac gcgcgagcga tggggctgct cgaggctctc ggcgtcgccc
     2281 atctggctga tcggctgtat ggcacgttgt ccgagggcga gcgcaagcgg gtgcagatcg
     2341 ctcgggcgct gatgacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttcg
     2461 gtctgccgtt ggtcgtcgag cggcacggca agcggtggag tgcccgcgcg gccgacccgg
     2521 actcgacacc ttcagcaggc tgacccaccc caaggaacct ctgtcgtggg gtaggcgtcg
     2581 tagggtgaag gagtcggtcg tctcggccgg ctctgcagag aggtgaagcg gtgtttcagg
     2641 ggtctagttg gctcatctgg gtcggagtgg ccttggcgct cggtgcgatc gaggccgcga
     2701 ccgtcgactt catcttcctc atgttggccg gcggtgcatt ggctggagca gtcgccgccg
     2761 ccttcggagc ggacttcccg ggccaagcga tcgtcgcgtc ggtctcggcg gtcgcgctcc
     2821 tcgtcggagt ccggccgtgg gtcaagcggc gattcaacgt caagaccgag cgccccacgg
     2881 tgggcatcgc cggaaacgtc gggcgcacgg caaccgtcct tgagaccgtg actcttgcgg
     2941 gtggccggat caacctctcc ggtgagacct ggtcggcccg ctcccagggc ggggacgcga
     3001 tcctgcccgg cgacaaggtc gaggtcatcg ccatcgacgg ggcgacggcc gtggtcgcgc
     3061 ccgtgactct gccaccccag cccctgccct gatcggcggg ctcccactcc tgcgcgttcg
     3121 accgttcgac cggcgagtag ccgccaagcc ccatcggagg aaccatgccc agaatcgaaa
     3181 caacgatcgt cctgctcctg ctgctcgtgt tcgcgatcac tgtcgtcgtc cggaccattc
     3241 ggatcgtgcc gcagcagacg gcggtgatca tcgagcggct cgggtcctac tcccgcaccc
     3301 tcgacgacgg catgcacgtc ctcgtgccgt tcatcgacaa gccccgagcg accatcgacc
     3361 tacgtgagca ggtcgtcacc ttccctccgc agccggttat caccagcgac aacctcgtcg
     3421 tgtccatcga cacggtcatc tactacacgg tgaccgacgc caagtcggcg gtctacgaga
     3481 tcgccaactt catccagggc atcgagcagt tgaccgtcac cacgctgcgt aacgtcatcg
     3541 gctccctcga ccttgaggag accctcacct cgcgtgacca gatcaacggg cagttgcgtg
     3601 gggtcctgga ccaggagact ggccgctggg gcatccgggt caaccgcgtt gagctcaagg
     3661 ccatcgaccc gccgcactcc gtgcaggact cgatggagaa gcagatgcgc gccgagcgtg
     3721 acaagcgtgc agcggtcctc accgccgagg gtgtcaagca gtcggcgatc ctcaccgctc
     3781 agggtgagaa ggaagcccag atcctgcggg ccgagggttc ggcccaggct cgggttcttg
     3841 aggctcaggg tcaggcgcag gccatccagc aggtcttcga tgcgatccac cgcggcaagc
     3901 caacccagcg gctgctggct taccaatacc tgcaggtgtt gccgcagttg gcccgcggcg
     3961 acgccaacaa gatgtggatc atcccctcgg agctcaccga cgccctgcgc ggtgtctcgt
     4021 cggcgctggg cggtaactcc tccggtgctg cgctcgagga agagtgggtc gagcccgctc
     4081 ccgctccggc cgcggctccg cctatccagc gcgccaagca gttcggtgag ccggagatcg
     4141 tcggggatgt tcacccgccg acccaccccg tcacgcagcc tgtcgcgccg ccgccgcctc
     4201 ctccgcggac tccggattcg gctcagtagg ccgcgccccg agttgcttcc ttagccagac
     4261 ctggatgccc tcggtgtcgg tgtcgggggc gggtgcgtgg gctcgccccc gcgttcaagc
     4321 ctttagtcgg gagtacacct aggccgccgg g
//
LOCUS       DAOMZY010000300         4340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_19100_length_4340_cov_5.224504, whole genome shotgun sequence.
ACCESSION   DAOMZY010000300 DAOMZY010000000
VERSION     DAOMZY010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19100_length_4340_cov_5.224504"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PLK64_14040"
     CDS             <1..260
                     /locus_tag="PLK64_14040"
                     /inference="COORDINATES: protein motif:HMM:NF018657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HPV80912.1"
                     /translation="TMVGAPVRTILGWVPTSGDQPIGVCLFTYNGTVGVGVCSDTRMI
                     PDPERVTELIRGHIDRLVAGAELRDEPPTPATSRRPTRSKA"
     gene            271..2331
                     /locus_tag="PLK64_14045"
     CDS             271..2331
                     /locus_tag="PLK64_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HPV80913.1"
                     /translation="MPRKRLVVVNITLQGPEWDFDEKVTFAGRDFRLIRVGTTGDLDA
                     AEKLVWQWSLKADAVAVTGVREAQASGVFKGDPLEVRQVLETTARVPVRDGSMLADVL
                     QEWAIRRVQAEMPGYFNNARTVVLSGKNYERTIRVLREFTANIDFVDPALRTDVPIAL
                     NTNPVVAKAAEVGMWTWRLTPGFFKDHSQGPVEWVSDKLAHRAARDADIIVGSYAELM
                     HFGLEDLAGKTVVTNAVSDGRLAELTSRGVDLIIDATPRPFQFMVVTAMLEAMAAAIN
                     TEERELTTDDLLELIQAAELEPRLLRPNGDKRKSRFAFVIHPLSAQYFNNVEAIGALS
                     RVPGMGDVVEKAMAYIPPFIYSHVTGIESPTGAEAEGWLISVGGTPKEMLAHSPEFTY
                     SRLLEAADLARKLGAQVMGLGAFTKVVGDAGVTVAKRAPLPVTTGNSYSASGALWAAH
                     AALRKLGIARVDDHGIIHGKAMVVGATGAIGSVCARLLALVTDELWLVSPETAKLLAI
                     KKDIESENPRAVLHVTARPGDALAEMDLIVTATSAAGNKVLDIMKVKPGCVITDVARP
                     LDMSAEDVAKRPDVLVIESGEIELPGDVHMGNIGLPAGVAYACLAETVVLALEGRYET
                     FTVGRNIEWPKVKEIYQLGLKHGMKLATISGVGGVFTDDDFARVREAALAARATASSD
                     ANAT"
     gene            2404..3240
                     /locus_tag="PLK64_14050"
     CDS             2404..3240
                     /locus_tag="PLK64_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019484019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPV80914.1"
                     /translation="MKSVQGKTVLITGAAMGMGRLFAERAIAEKAKAVVLWDLNEVAL
                     QSTLEELTGRGSEVTAYLVDVGDLAAVEASAAAVLAEVGAVDVLVNNAGVVRGNQYFW
                     ESDSGRDTRLTVAVNTLAPMYVARAFLPGMIAGSGEARLLNLASAAGFTANPRMAVYA
                     ASKWAVIGWSDSVRLELKQAGVEHVKVTTVCPYYVNTGMFDGAKSAPLLPILEPAHVV
                     DEAWRAMCAGGSFVVLPRTVMLSEVLKGLVPISVRDFISDNVIGVYHTMEDFTGRAEQ
                     QA"
     gene            3279..4166
                     /locus_tag="PLK64_14055"
     CDS             3279..4166
                     /locus_tag="PLK64_14055"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPV80915.1"
                     /translation="MAQRNHRVPVSYGASIMALEVLPARPVAEGVPTVVLAHGWCLDH
                     TSWHKVVADLQARRNVRVVLFDQRGHGKSSMGEVGDPTVRILGDDLREVIDAVAPEGD
                     LVLAGHSMGGMSVMAYAGMHYEHFTERVKGTVLASTAASIEGRTPVPLERVIMALASR
                     APGIQPRVFIPRMVEGKLIFGPGAKREDIRHAVDQIKHTKMPTIGRFFYAIAGHDEMD
                     ALGHFVDVPTHIIAGNHDRLIPVPHAEALRKRIPGADLNVLDGVGHMTTYEASSIICD
                     AIVGLVDSSVPPKPRRARR"
BASE COUNT          725 a         1469 c         1451 g          695 t
ORIGIN      
        1 tcaccatggt cggcgccccg gtccgcacca tcctgggctg ggtccccaca tccggcgacc
       61 agccgatcgg cgtctgcctg ttcacctaca acgggaccgt tggggtcggg gtctgctcgg
      121 acacccggat gatccccgac cccgagcggg tcaccgagct catccgcggt cacatcgacc
      181 gactcgtcgc gggggccgaa ctgcgcgacg agccaccaac ccccgcaacg tctcgccgcc
      241 ccacccgctc gaaggcctag gagatccggt atgccgcgca aacggcttgt cgtcgtcaac
      301 atcaccctgc agggccccga gtgggacttc gacgaaaagg tgacctttgc cggtcgggac
      361 ttccgactca tccgggtcgg gacgaccggc gacctcgacg ctgccgagaa gctcgtgtgg
      421 cagtggtcgc tcaaggccga cgccgtcgcg gtcaccggtg tccgtgaggc gcaggcctcc
      481 ggcgtcttca agggtgaccc tctcgaagtg cgtcaggtcc ttgagacgac cgcacgggtg
      541 cccgtccgcg atgggtcgat gctcgctgat gtgttgcagg agtgggcgat ccgccgggtg
      601 caagccgaga tgcctggcta cttcaacaat gccaggacgg tcgttctcag cgggaagaac
      661 tacgagcgga cgatccgggt cctgcgggag ttcaccgcca acatcgactt cgtcgacccg
      721 gccctgcgga ccgacgtccc catcgcgttg aacaccaacc cggtggtcgc caaggcggcc
      781 gaggtcggga tgtggacctg gcgcctcacc ccggggttct tcaaggacca cagccagggg
      841 ccggtcgagt gggtgagcga caaactcgcc caccgggccg cgcgggacgc ggacatcatc
      901 gtcgggagtt acgccgagct gatgcatttc gggctcgaag acctcgctgg caagaccgtc
      961 gtgaccaacg ccgtgtccga tggccgactg gccgagctca ccagccgtgg tgtcgacctg
     1021 atcatcgacg cgacgccacg ccccttccag ttcatggtgg tcaccgcgat gctcgaggcg
     1081 atggccgcag ccatcaacac cgaggagcgg gagctcacca ccgacgacct cctcgaactg
     1141 atccaggccg ccgagctcga accccggctg ctgcgtccca acggcgacaa gcgcaagagc
     1201 cgattcgcct tcgtcatcca tccgttgagc gcgcagtact tcaacaacgt cgaagcgatc
     1261 ggtgccttga gccgggttcc cgggatgggc gacgtcgtcg agaaggcgat ggcctacatc
     1321 ccgccgttca tctacagcca cgtcacgggc atcgaatcac ccaccggtgc cgaggccgag
     1381 ggctggttga tctcggtggg cggcacgccc aaggagatgc ttgcccacag cccggagttc
     1441 acctactcac gtctgctgga ggctgccgac ctggcccgga agttgggcgc ccaggtgatg
     1501 gggctcggtg ccttcaccaa ggtcgtcggc gacgccggcg tcaccgtggc caagcgggca
     1561 ccgctgccgg tgacgacggg gaacagctac agcgcctccg gggcactctg ggcagcccac
     1621 gcggccctgc gcaaactggg catcgcccgc gtcgacgacc acggcatcat ccacggcaag
     1681 gccatggtcg tcggggccac tggggcgatc ggctcggtct gtgcccggct gttggcgctc
     1741 gtcaccgacg agttgtggtt ggtctcgccc gagaccgcca agttgctggc catcaagaag
     1801 gacatcgaga gcgagaaccc ccgggccgtc ttgcacgtca ccgctcgacc gggcgacgca
     1861 ctcgccgaga tggacctcat cgtcaccgcc acctccgccg cggggaacaa ggtgctcgac
     1921 atcatgaagg tcaagccagg gtgcgtcatc accgacgtgg cccggccgtt ggacatgtct
     1981 gccgaggatg tcgccaaacg tcccgacgtg ctcgtcatcg agtccggcga gatcgagttg
     2041 cccggtgacg tccacatggg caacatcggc ctgcctgctg gcgtcgccta tgcctgcctc
     2101 gccgagaccg tcgtcctcgc gctcgaaggg cgctacgaga cgttcacggt cggacggaac
     2161 atcgagtggc ccaaggtcaa ggagatctac caactcgggc tcaagcacgg catgaagttg
     2221 gcgacaatct ccggcgtcgg cggcgtattc accgacgacg atttcgcccg agttcgcgag
     2281 gccgccctgg ccgcacgcgc caccgcgtca agcgacgcca acgcgacgta ggccacgtcg
     2341 cctgggccat gtcactaggt gctcttcctg tcaggatggg gagagacccg atggaggagt
     2401 gtcgtgaagt cagtccaagg aaagacggtc ctgatcaccg gtgccgcgat gggcatgggg
     2461 cgcttgttcg ccgagcgagc catcgccgaa aaggccaagg cggtggtcct ctgggatctc
     2521 aacgaggtcg ctctgcagag caccctcgaa gagttgacgg gacgcggaag tgaggtcacg
     2581 gcatacctcg tcgacgtcgg cgatctcgcc gcggtggagg ccagcgccgc tgccgtcctg
     2641 gccgaggtcg gggcagtgga cgtcctggtc aacaacgccg gtgtcgtccg cggcaaccag
     2701 tacttctggg agtccgactc ggggcgggac actcgcctga cagtggcggt caacaccctc
     2761 gcccccatgt atgtcgcgcg ggccttcctt ccggggatga tcgccggcag cggggaggcg
     2821 cgcctgctca acctcgcctc ggccgccggt ttcacggcca acccgcggat ggccgtgtat
     2881 gccgcgtcca agtgggcggt catcggctgg tccgactcgg tgcggctgga gctcaagcag
     2941 gccggggtcg agcacgtcaa ggtgacgacc gtgtgccctt attacgtcaa caccggcatg
     3001 ttcgacggcg ccaagtccgc gccgctcctg ccgatcctcg agccggcgca tgtcgtcgac
     3061 gaggcctggc gggcgatgtg tgccggcgga tcgttcgtcg tcctgccgcg caccgtcatg
     3121 ctcagcgagg tgctcaaggg cttggtgccg atcagcgttc gcgacttcat ctcggacaac
     3181 gtcatcgggg tctaccacac gatggaggac ttcaccggcc gcgccgagca gcaggcgtga
     3241 ccaggtcgtc ggacccagca ggtcggaagg actgagcctt ggcgcagcgc aatcaccgcg
     3301 tcccggtctc ctacggtgcc agcatcatgg cgctggaggt cctccctgcg cgcccggtcg
     3361 cggagggtgt gccgaccgtc gtgctggccc acgggtggtg cctggaccac acgagttggc
     3421 acaaggttgt cgccgacctt caggcccggc ggaacgtccg ggtggtgctc ttcgaccagc
     3481 gggggcacgg caagtcgagc atgggcgaag tcggcgaccc cacggtccgc atcctcggtg
     3541 acgacctgcg cgaggtcatc gacgcggtgg cgcctgaggg tgatctcgtc ctggccgggc
     3601 actccatggg tggcatgtcg gtgatggcct acgccgggat gcactacgag cacttcaccg
     3661 agcgggtcaa gggcaccgtg ctcgcgtcga cggccgcgtc gatcgagggt cgcaccccgg
     3721 tgccgctgga gcgggtcatc atggcgttgg cctcgcgggc tccgggcatc cagcctcggg
     3781 tcttcatccc ccgcatggtc gaggggaagc tcatcttcgg gccgggcgcc aagcgtgagg
     3841 acatccggca cgcagtcgac cagatcaagc acacgaagat gccgacgatc ggccggttct
     3901 tctatgcgat cgccggtcat gacgagatgg acgccttggg ccatttcgtc gacgtgccga
     3961 cccacatcat cgccggcaac cacgaccggc tgatccccgt cccgcacgcc gaggccctgc
     4021 gcaaacggat ccccggcgcc gacctcaacg tcctcgacgg ggtgggtcac atgacgacct
     4081 acgaggcgtc gagcatcatc tgcgacgcga tcgtcggcct agtcgactcc agcgtgcccc
     4141 cgaagccccg ccgcgcccgc cgctgatcac cgcgctgggt cgggcgcgcc gagtcggctt
     4201 gcatgaggtt agtgacgctt gtatgaggct gtaacggcat acaagcgtca ctagcctcat
     4261 acaagcggtg agcagctgcg cggaaggcgt gcggagggcg tggctaacgc ctcaccagcc
     4321 gcgggcgagc cactcggcga
//
LOCUS       DAOMZY010000301         4336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_19127_length_4336_cov_6.404345, whole genome shotgun sequence.
ACCESSION   DAOMZY010000301 DAOMZY010000000
VERSION     DAOMZY010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19127_length_4336_cov_6.404345"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1414)
                     /locus_tag="PLK64_14060"
     CDS             complement(<1..1414)
                     /locus_tag="PLK64_14060"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPV80916.1"
                     /translation="MRAEREISSGRKSRARVLAVLSTSTRLRIAIAAVVLVVNALLGD
                     GSLVLPWALLVAGVDVVAGFLLAHEDVLLPHRSQRAAVAATVIGAFAAGLSLLAGAGA
                     LPLFVIPLYRAGERGGRGGVAMTGGALAVGAGIAQTVGPPAVLPLGQVVLWGALAVSL
                     GTLAAWEDRITPRRRLRDPIADEAAIVLGRLAQLSAGMDGGLDHTVMAERILDHLPSK
                     PGLRGVVLAGTPVSVAIPIALRGAARVPWPEPREDDHNTRQRAWTLGVPGSDVEDNRA
                     VVAVPLRDVRGEQVGMLVADWPARTPATAAEVLEITEAAERYSTGLAVAMSYAHLREQ
                     AGMDERRHLARAMHDGIAQEIAAVGFHIDMVRYAAQQAGDSTAPELANLRAEVARILI
                     DLRNDITDLRVGLRPEHGLGAAVSARLQQFGATTGAVVTVVLRESGFRLPAATEVSLY
                     RLLLDVLADASRHRANHVEVE"
     gene            1556..2284
                     /locus_tag="PLK64_14065"
     CDS             1556..2284
                     /locus_tag="PLK64_14065"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HPV80917.1"
                     /translation="MDFWRRTRHGAAALLDLVLPAECAGCGGAGVCWCATCACDLAAA
                     CFPLPRHTPPSPCPPGMPPVVAAARYTGALRAAIVAVKDGGRADARPLLTPLLAQAIL
                     VAAARREDLALVPMPSSRAAVRRRGEASVTVLCRQADAALGHPHEVVEALSPSRRVAD
                     QAGLGSAERRANLVGAYAVPPRWRDTVAGRDVLLVDDVVTTGATLTEGARALRAAGAH
                     VVGAAVVAATQRRGRADVPSGLTR"
     gene            2393..3028
                     /gene="raiA"
                     /locus_tag="PLK64_14070"
     CDS             2393..3028
                     /gene="raiA"
                     /locus_tag="PLK64_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPV80918.1"
                     /translation="MDIVVTGRHIQVSERFRAHLDDRLAKVAQMAPKVSRVDVVVTHE
                     RTARNSEFVELTCRDRGSIVRAEAASEDKYNALDQAVEKLLERLRRVNGRRRAAVRAG
                     RPDVGLAAVAAAPAREEAQAAPVEAPPDQATGPFGAVGDSPIEVREKVHPSEPMTLAE
                     AVNRMELVGHDFYLFNDADTGRASVVYRRRGWSYGVIHLEPAVAHDTQASG"
     gene            3063..3755
                     /locus_tag="PLK64_14075"
     CDS             3063..3755
                     /locus_tag="PLK64_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPV80919.1"
                     /translation="MTDPWPRDHPIRVLIADDHALFRRGLRTVLELDGDIVIVGEADD
                     GMAAVEKAEATVPDVVLMDVRMPRCGGIEACQAIRERVPSARIVMLTMSDEESDLFEA
                     VRAGANGYVLKDVPGDEIAAGIRAVQSGDSLISPSMASKLLAEFAQMRRRDVEVVDSH
                     LPKLTDREVEVLGLVARGLGNREIGRELFISENTVKNHVRNILEKLQLHTRMEAAMYA
                     VQQNLIHPPPAG"
     gene            3795..>4336
                     /locus_tag="PLK64_14080"
     CDS             3795..>4336
                     /locus_tag="PLK64_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006215411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HPV80920.1"
                     /translation="MRELTGAQARRIALAAQGLTAPRPASGSVGTRHLGGVIDRLGVV
                     QIDSVNVVARSHYLPFFSRLGPYDREALDRMRDAAPRRLVEYWAHEAALVAPATWPLF
                     AGRMRAAESEAWGGMRQVHAEHPGLVEAVLREVTDRGPLTSRQVEAALAHDVPRSRED
                     WGWNWSAVKRALEWLFWSGQV"
BASE COUNT          638 a         1491 c         1543 g          664 t
ORIGIN      
        1 gttcgacctc gacgtggttg gcgcggtggc gcgaggcatc ggcgagtacg tccagcaaca
       61 gccggtagag gctcacttcc gtcgctgccg gcaaccggaa gcctgactcg cgcagcacaa
      121 ccgtgacgac ggcaccagtg gtggcaccga actgctgtag ccgggccgac acagcggccc
      181 cgaggccgtg ctcagggcgc aatccgaccc gtagatcggt gatgtcgttg cgcaggtcga
      241 tgaggatgcg cgcgacctct gctctcaggt tggccagttc cggtgccgtc gagtctccgg
      301 cctgctgcgc ggcataccgg accatgtcga tgtggaagcc gaccgccgcg atctcctggg
      361 cgatgccgtc gtgcatggcg cgagccaggt gccgacgctc gtccatcccg gcttgctcgc
      421 gcaggtgggc gtaggacatc gcgacggcga gaccagtcga atagcgctcg gcggcctcgg
      481 tgatctccag gacctcggca gcggtcgcag gcgttcgggc cggccagtcg gccacaagca
      541 tcccgacctg ctcaccgcgg acatcgcgca gcgggaccgc gacgaccgca cggttgtcct
      601 ccacatcgct gccaggcacc cccaaggtcc aggcccgctg ccgcgtgttg tggtcatcct
      661 cgcgaggctc gggccacgga actcgagctg ctccgcgcag agcgatgggg atggccaccg
      721 acaccggggt gcctgcgagg acgactcccc gcaggcccgg cttgctcggc aggtggtcga
      781 ggatccgttc ggccatgacc gtgtggtcga gaccgccatc catgcccgca gacagttgcg
      841 cgaggcggcc gagcacgatg gcggcctcgt cagcgatcgg gtcgcggagg cgccggcggg
      901 gagtgatgcg gtcctcccac gcggccagcg tgccgaggct caccgctagc gctccccaga
      961 gcaccacttg gcccaggggc aacacggctg ggggaccgac ggtctgggcg atcccggcgc
     1021 cgacagccag cgcgcccccg gtcatcgcca ccccgccgcg accaccgcgc tcgccagcgc
     1081 gatagagagg gatgacgaac aacggcaggg cacctgcgcc tgcgagcaac gacaggcccg
     1141 cggcgaacgc gccgatcacg gtcgcggcga ctgcggctcg ttgggatcgg tggggcagca
     1201 ggacatcttc atgggccagc aggaagccag ccaccacgtc gactccggcg accagcagcg
     1261 cccagggcag cacgaggctg ccgtcaccca ggagggcatt gacgacaagg acgactgctg
     1321 cgatggctat gcgcagtcgg gtcgacgtac tcaggactgc cagcacccgc gctctggact
     1381 ttctccccga ggagatctcc cgttccgccc tcatgatgac acctcgcccg tggtccgagg
     1441 gtggaattga cgggttcggt gccccttgtc cacaccgcgt gctcccgtcg tcccactgtc
     1501 cacaggcaga cccggcggac ggggcgcccc gtcctgcaat ggtcaccatc aacccatgga
     1561 cttctggcgg cggacacgac acggcgcagc ggcgctgctg gacctcgtcc tcccggccga
     1621 atgcgcgggt tgcggtgggg cgggcgtttg ttggtgtgcc acctgcgcgt gcgacctggc
     1681 cgccgcctgc ttcccgctgc cgcggcatac cccgccgtcg ccatgcccac ccggtatgcc
     1741 gcccgtcgtc gccgccgccc gatacaccgg tgcactccgg gccgcgatcg tggccgtcaa
     1801 ggacggtggc cgggccgacg cgcgcccgct gctcacgccg ctgctcgccc aggcgatcct
     1861 cgtcgccgcc gcccgacgtg aggacctcgc gctggtgccg atgccctcct cgcgagcggc
     1921 cgttcgccgt cgcggggagg cgagcgtcac ggtgctgtgc cggcaggccg acgcggcgct
     1981 tggtcatccg cacgaggtgg tggaggccct ttccccctcg cgtcgcgtcg ccgaccaagc
     2041 aggtctcggc agcgccgagc ggagggccaa cctcgttggt gcgtatgccg ttccgccgcg
     2101 gtggcgcgac acggttgccg gccgcgacgt cctgctcgtt gacgacgtcg tcacgacggg
     2161 ggcgacgctc accgaaggcg cgcgggcgct gcgcgcggcg ggcgcgcatg tcgtgggggc
     2221 ggctgttgtc gcagcgacgc aacgccgtgg gcgcgccgac gtgccctctg gcttgactcg
     2281 gtgaagcggg ctacggtgga aggacatcgt ccgagacgtc tgggggtgat gatcaggttt
     2341 gcggggtctg cccggcccgc cacctcgtca cgtcgcacgt caaggaggaa cagtggacat
     2401 cgtcgtcacc ggtcgtcaca tccaggtttc agagcgtttc cgggcacacc tggacgacag
     2461 gctggccaag gtcgcacaga tggcgcccaa ggtctcacgg gtcgatgtcg tcgtcaccca
     2521 tgagcgcacg gctcgcaact cggagtttgt cgagctcacc tgccgcgatc gggggtcgat
     2581 cgtgcgcgcc gaagcggcca gtgaggacaa gtacaacgcc ctcgaccaag ccgtcgagaa
     2641 gctcctggag cggctgcggc gggtcaacgg gcggcgccgg gctgcggtgc gcgcggggcg
     2701 ccccgacgtg ggcctggctg cggtggctgc ggcgccggcg cgagaggagg cgcaggccgc
     2761 accggtcgag gctccgcccg accaggccac cggcccgttc ggagctgtcg gcgactcgcc
     2821 cattgaggtg cgcgagaagg tccacccgag cgagcccatg actctggccg aggcggtgaa
     2881 ccggatggag ttggtcgggc atgacttcta cctgttcaac gacgccgaca ctggccgcgc
     2941 gagcgtcgtc tatcggcgcc gtgggtggtc ctacggggtc attcatctgg agcctgcagt
     3001 cgcgcacgac actcaggcat ccggctgagg cgcgcgtcac atgggcatga catcatgccc
     3061 atgtgaccga cccatggccc cgtgaccacc cgattcgggt gctcatcgcc gatgaccatg
     3121 cacttttcag gcgtggcctg cgcacggtcc ttgagctcga cggtgacatc gtcatcgttg
     3181 gcgaggccga cgatggcatg gctgctgtcg agaaggccga ggccaccgtc cccgacgtcg
     3241 tcctcatgga tgtccgaatg cctcgctgcg ggggcatcga ggcctgccag gcgatccgag
     3301 agcgggtgcc gtcagcgcgc atcgtcatgt tgacgatgtc cgacgaggag tcggatctct
     3361 tcgaagcggt gcgcgctggc gccaacgggt acgtcctcaa ggacgtcccc ggcgatgaga
     3421 ttgcggcggg catccgcgct gtgcagagcg gcgattcgct catctcgccg tccatggcct
     3481 ccaaactgct tgccgagttc gcccagatgc gccgccgcga cgtcgaggtc gtcgacagcc
     3541 acctgcccaa gctcacggat cgcgaggtcg aggtgctcgg cctggtggcc cgcggcctgg
     3601 gcaaccgcga gatcggccgc gagttgttca tctcggagaa caccgtgaag aaccacgtcc
     3661 gcaacatcct ggagaagctc cagttgcaca cccggatgga agcggcgatg tatgccgtgc
     3721 agcagaacct cattcacccg ccaccggccg ggtgagcggc ggcatacctg ctgggggagt
     3781 gcctcgggtg agcgatgcgt gagctgactg gagcgcaggc ccggcggatc gcgttggccg
     3841 ctcaagggtt gaccgcgccg cgtcccgcat ccggctcggt tggcacccgg cacctgggcg
     3901 gcgtcattga ccggctcggc gtcgtccaga tcgacagcgt caacgtcgtg gcgcgcagtc
     3961 attacctgcc gttcttcagc cgcctcggcc cctatgaccg cgaggcgttg gatcggatgc
     4021 gcgacgccgc gccgcgtcgt ctcgtcgaat actgggcgca cgaggcggcc ctcgtcgcgc
     4081 ccgcgacctg gccgctgttc gccggtcgga tgcgggctgc ggagtccgag gcgtggggag
     4141 gcatgcgtca ggtccacgcc gaacacccag gcctcgtcga ggccgtgctg cgggaggtga
     4201 ctgatcgcgg cccgctgacc tcgcgccagg tggaggccgc gctcgcgcac gacgtaccgc
     4261 gatcccggga ggactggggg tggaactggt cggcggtgaa gcgggcgttg gagtggctct
     4321 tctggtcggg gcaggt
//
LOCUS       DAOMZY010000302         4332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_19151_length_4332_cov_4.684124, whole genome shotgun sequence.
ACCESSION   DAOMZY010000302 DAOMZY010000000
VERSION     DAOMZY010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19151_length_4332_cov_4.684124"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..231)
                     /locus_tag="PLK64_14085"
     CDS             complement(43..231)
                     /locus_tag="PLK64_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80921.1"
                     /translation="MSLAFLLTSLAIVATPGTGAILTISTGLRGGRRLAVITAVGCTL
                     GIVPHLAACSSAPRYSWR"
     gene            complement(228..617)
                     /locus_tag="PLK64_14090"
     CDS             complement(228..617)
                     /locus_tag="PLK64_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HPV80922.1"
                     /translation="MPEYLISFNDEWVAEHTPEQIDGKATASRALIQEMREAGVLLFA
                     NGALDRSTVLCSVRAADGEPVFADGPAVERDEYLGGFTVIDVPDDQSARHWAGRLAVV
                     LDWPQEVHRFRGPGEGASHQSGRDRQL"
     gene            complement(672..1583)
                     /locus_tag="PLK64_14095"
     CDS             complement(672..1583)
                     /locus_tag="PLK64_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80923.1"
                     /translation="MCDLLDTPEPTWRAARATVETTGYGAALLSHQDPDGLWAGGVFV
                     PDGFTRELWQHEGQPWTASAYAMNDLREWGLEPTSEAAVRTVRFVGANGRWDEGDQPF
                     WQGEVEECINGRTLADGAYFGVDMAPLAARLVAEQQPDGGWNCERANGSVRSSFDSTI
                     NVLEGLLEFERATRGTTESQAARRGGEEFLLERGLFRRRTTGEPADAAYILLGHPRRW
                     HYDVLRALDYFRASAILAGTAQAPVDPRLADALVHLRGRRLPDGRWPLDVRHRGRQWF
                     EVDEGVGMPSRWVTLSALRVLRWADMQ"
     gene            complement(1815..2369)
                     /locus_tag="PLK64_14100"
                     /pseudo
     CDS             complement(1815..2369)
                     /locus_tag="PLK64_14100"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
     gene            complement(2603..2992)
                     /locus_tag="PLK64_14105"
     CDS             complement(2603..2992)
                     /locus_tag="PLK64_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80924.1"
                     /translation="MATDADVERIAMALPEVELGTFWGGPAYVVAGKAIANRRVPRRD
                     EGTVDPATDEPYQDLLVIHLGDRETKEQVLATFDPDVVFTIPHFARSTAVLAHLDAIA
                     IDDLEDLLDLAWTGRAPKRLVAERDAR"
     gene            3129..4100
                     /locus_tag="PLK64_14110"
     CDS             3129..4100
                     /locus_tag="PLK64_14110"
                     /inference="COORDINATES: protein motif:HMM:NF020795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwqG family protein"
                     /protein_id="HPV80925.1"
                     /translation="MWDRLFGKGWAKAAAPEDVEAPVEELEWEEAPASVEQFTARLSA
                     VGLGEHTDALVALVRPSLRLVADEDVDDPQAAESAESAGLRIGGRPLLAPGVDWPRRK
                     DGAPLSLVAQISLADVAELADGEDQADFPTEGSLAFFYDAVDQSVWGFSPTDSEGWAV
                     LHCPDGVGALRDYPAELDEEGRFSPIALTPELEQTYPPAASFEVEQLVGATKAEKYWP
                     VLGEDEGAGTITRFLGHPDVIQGEMQVECQLASNGVYVGDSKGYTSPDGIRLRPGAAE
                     WRLLLQVDSHEDAGMMWGDSGRLYWWIREADLAEGRWDATWLVLQCY"
     gene            4091..>4332
                     /locus_tag="PLK64_14115"
     CDS             4091..>4332
                     /locus_tag="PLK64_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPV80926.1"
                     /translation="MLLKLLVLGGTAFLGRAIAGYAVAQGHEVTCLARGSAPAPTGAR
                     LVVGDRDRDDTFEALADDAWDAVIDVTRHPGQARRAV"
BASE COUNT          656 a         1453 c         1574 g          649 t
ORIGIN      
        1 tcgccgcagc gaaccagccg tacccagcga agacaaccaa ggtcatcgcc atgaataccg
       61 cggagcggac gagcacgcgg ccaggtgcgg gacgatgccc agggtgcagc cgaccgcggt
      121 gatcaccgcg aggcggcgcc ctcctcgcag gccggtactg atcgtcagga tcgctccggt
      181 ccccggcgtc gcgacgatcg ccagggacgt caacaggaag gcgaggctca tagctgacgg
      241 tcccggccgg actgatggct ggccccctca cccggcccgc ggaaccggtg gacctcctgc
      301 ggccagtcga gcacgacggc gagccgcccc gcccagtgcc gcgcgctctg gtcgtctggg
      361 acgtcgataa ccgtgaatcc gccgagatac tcgtcccttt cgacggcggg gccgtcggcg
      421 aagaccggct cgccatccgc ggcccgcacg ctgcacagga ccgtggaccg gtcgagcgca
      481 ccgttggcga acagcaggac ccccgcctcg cgcatctctt gaatcaaagc cctcgaagca
      541 gtagctttcc catcgatctg ctcgggcgta tgctccgcca cccactcgtc gttgaacgag
      601 atcaggtact ccggcacgtc gactccttgc gagtggtgga cagcctcggc ctgcaccaac
      661 agcccctcgg cctactgcat gtcggcccat cgaagcaccc gcagcgcgga cagcgtgacc
      721 cagcgggacg gcatacccac accctcgtcg acctcgaacc actgccgccc gcgatgccgc
      781 acgtccagcg gccagcgccc atccgggagg cgacggccgc ggaggtggac gagcgcgtcg
      841 gccaggcgcg gatcgacagg ggcctgcgca gtgccggcga ggatcgcgct ggcacggaag
      901 tagtcgaggg cgcgcaacac gtcgtagtgc caccgacgag ggtgcccaag caggatgtat
      961 gccgcgtccg ccggctcgcc cgtagttcgg cggcggaaca gcccccgctc gaggaggaac
     1021 tcctcaccgc cacgccgggc cgcttgagac tcggtcgtgc ccctggtcgc ccgctcgaac
     1081 tccagcagcc cctcgaggac gttgatcgtc gagtcgaagg acgagcggac cgatccgttg
     1141 gcgcgttcgc agttccaccc gccgtcgggt tgctgctcag ccacgagccg cgcggcaagt
     1201 ggcgccatgt cgaccccgaa gtaggcgccg tcggcgaggg tccggccgtt gatgcactcc
     1261 tcgacctcgc cctgccagaa cggctggtcg ccctcgtccc accggccgtt cgcgccgaca
     1321 aaccgcaccg ttcgcacggc ggcttcggag gtcggctcca gaccccattc gcgcaggtcg
     1381 ttcatcgcgt atgccgatgc cgtccagggc tgtccctcgt gttgccacaa ctcgcgagtg
     1441 aagccgtcgg ggacgaagac accgcccgcc cagaggccgt cggggtcctg atgggacagg
     1501 agggccgcgc cgtaaccagt ggtttcgacc gtggcgcggg cggcgcgcca ggtcggctcg
     1561 ggcgtgtcga gcaggtcgca catgacctgc caacggatgg ccgggtcgcc gtcgagcagc
     1621 cagtcgatca cctcgtcacc aagcccgttt gccgaaaggt gggattcggt cgcttcggga
     1681 cgattcgaag cgaccgtttg ccacccctcg gtggcgtcgg tggcgtcggt ggcgtgggcg
     1741 gcaggcgcgt agggcgcgga actcgtcgga attgcggtga gggcgtcgag gtcgcggacg
     1801 gcatacctga ggtgttccca ctcctcgtcg aggatggtcc ggaggcacga gagcaccgtc
     1861 ttctcatgct caggtgcgtg ggggttgtgc cgaggcgcgg caagagtgtc ggcggacacc
     1921 tgcgcaagga acgtggcgac catcgcctgc cgttcggccc gcgcctgcaa gaccacctcg
     1981 aacgtcggct ccgcgtcggc gaattcggac ccgtcgtatg ccgcggaccc accgccgtcg
     2041 tcgttgggca ggccagccgg gtggtagggg tgctcgcgct gcaggaccgc cttgccgagc
     2101 caggtgtcgg tggccatgac gaggtggcgc agggtctggg cgaaggacca ctcgccggcg
     2161 accgaggcgt ccacggtgcc gggcggcata ccggtcgccc gttggagggt cgcggcccag
     2221 gccgccgcca gcgcggccca ggcggcgcgg aggccttcgg ggtcggtggc ggtgcgcagt
     2281 tcgcgcccgg ggaaccggcg gttcagctcg gcatcgacca gggggacgac gtcgacgccg
     2341 ttgacgagca ggcggctgcc gtcctccatg agccacgggg agtcgatctc catgccggcc
     2401 acctcgctgc cgcggacgac gacctcgccg aggtcacagt cgacgaagcg cgaaccgcgc
     2461 agcgtgcggt gagtgaaggt ccgaccagcg agcggttcgg gcggagtgtc agccatggca
     2521 tgactgtgcc atggtgcgcc gacagtgggg cgacaattcg ggcgcggaac tgatggcgtg
     2581 cgtcagtgtg agtcagtgtg agtcagcggg cgtcgcgctc ggcgacgagc cgcttcgggg
     2641 cgcgaccggt ccaggcgagg tcgagaaggt cctccaggtc gtcgatcgcg atcgcgtcca
     2701 ggtgcgcgag gacggccgtc gagcgagcga agtgcgggat cgtgaagacg acgtccgggt
     2761 caaaggttgc caggacctgc tctttcgtct cacggtcgcc gaggtggatg acgaggaggt
     2821 cttgatacgg ctcgtcggtg gctgggtcga cggtcccttc gtcgcggcgc ggcacccgcc
     2881 ggttcgcgat cgccttgccc gcgacgacgt atgccggccc gccccagaag gtgcctaact
     2941 ccacctcggg caaggccatc gcgattcgct ccacgtcagc gtccgtcgcc atcgagccag
     3001 tatgccgtcc ggcgcggcat accggattgt gtggctggaa ctcccccacg ggacgttgga
     3061 gccacacgat cttcgccgca ctgagttgcg cggacgggcc tggtgcacgc gcggggcaga
     3121 atcagaccat gtgggatcgg ttgttcggga agggctgggc caaggccgct gcgccggagg
     3181 acgtcgaggc gcccgtcgag gagctcgagt gggaggaggc accggcgtcg gtcgagcagt
     3241 tcaccgcccg gctgtcggcg gtggggctgg gcgagcacac ggatgccctc gtcgccctgg
     3301 tccgccccag ccttcggctc gtggccgacg aggacgttga cgacccgcag gcggcggagt
     3361 cggcggagtc ggccggcttg cgcatcggcg ggcggccgct gctggccccc ggggtggact
     3421 ggccgcgacg caaggacggc gcacccctga gcctggtcgc gcagatctcg ctagcggacg
     3481 tcgccgagct ggcggacggg gaggaccagg cggacttccc gaccgagggc tcgctggcgt
     3541 tcttctacga cgcggtcgac caatcggtct gggggttcag cccgaccgac tcggaggggt
     3601 gggcggtgct gcattgcccg gacggggtcg gcgcgctgcg cgactatccg gctgagctcg
     3661 acgaggaggg ccggttctcg ccgattgctc tcactcccga gttggagcag acctacccgc
     3721 cggccgcgtc gttcgaggtc gagcagcttg tgggcgccac gaaggccgag aagtactggc
     3781 ccgtcctcgg cgaggacgag ggcgccggga cgatcacccg gttcttgggc catcccgacg
     3841 tcatccaagg cgagatgcag gtcgaatgcc agttggcgag caacggtgtc tacgtgggtg
     3901 attcaaaggg ttacacgtct cctgatggca tccgattgcg gcctggtgcg gccgagtggc
     3961 gcctcctcct gcaggtcgac agccacgaag acgccgggat gatgtgggga gactccggcc
     4021 ggctctactg gtggatccgc gaggccgatc tcgccgaagg ccgttgggac gcaacctggc
     4081 tcgtcctcca gtgctactga aacttctcgt cctcggcggc accgcgttcc tcggccgagc
     4141 gatcgctggg tatgccgtgg cgcagggtca cgaggtcacc tgcctggcgc ggggcagcgc
     4201 gcctgctccc acaggggcac gactcgtcgt gggggatcgg gaccgggacg acactttcga
     4261 ggcactcgcc gacgacgcgt gggatgcggt catcgatgtg actcggcacc ccggtcaggc
     4321 ccgacgggcg gt
//
LOCUS       DAOMZY010000303         4278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_19479_length_4278_cov_6.147289, whole genome shotgun sequence.
ACCESSION   DAOMZY010000303 DAOMZY010000000
VERSION     DAOMZY010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19479_length_4278_cov_6.147289"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="PLK64_14120"
     CDS             complement(<1..767)
                     /locus_tag="PLK64_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HPV80927.1"
                     /translation="MAEPAVVRQWRSARPVQLAAILAPFRRGGGDTTFRVGPDGSVVR
                     GIQTPDGPATLELNCAPKDSEISARAWGPGAAWAVEQAPALVGEGDDPSGFVGLHPPV
                     VDAIRRFPGWRVPRSGLVLDALVPAIIEQKVTGKEAFGGYHRLVRIHGDVAPGPWGAA
                     GLRVPPDAAGWRAIPSWEFLRASVDASRSDTIQRVCRVAGSLERLTSGSTEAGRAGLR
                     SVSGVGVWTVAEVAQRAWGDADAVSFGDYHVARTIGWT"
     gene            800..1513
                     /locus_tag="PLK64_14125"
     CDS             800..1513
                     /locus_tag="PLK64_14125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03089 family protein"
                     /protein_id="HPV80928.1"
                     /translation="MPTRSSMPADVLAAMLRLDPGRPRITCYDDASGERIELSAKTFA
                     NWVAKAANLLQDSGDVTVGNTVGLDLPAHWRTAYWAMAAWSVGCIVVVGPEARDADVV
                     VTVSPDLAAECVTDGRYAVLVTLAALARGNAQTPQDVLDEAKELAGYGDRFDPWDTPE
                     ADEPALVGDGDDAAYAQVVVRRDWPERPRVLVSGDRLGSALRAFLSAWAADGSVLLVL
                     NPAGDQDRRRTIENVTATA"
     gene            complement(1519..2160)
                     /locus_tag="PLK64_14130"
     CDS             complement(1519..2160)
                     /locus_tag="PLK64_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HPV80929.1"
                     /translation="MALTREEMAARAARELHDGDYVNLGIGLPTLVPNYVPDDVEIVL
                     QSENGILGVGAYPFEGEEDPDLINAGKETVTIRRGASFFDSATSFGMIRGGKVNAAIL
                     GAMQVSAAGDIANWMIPGKMVKGMGGAMDLVHGAQHVIVLMEHVARDGAHKIVQECSL
                     PYTGKRVVHRIITDLAVLDVTPDGLVLRELAPGVTLDEVRSATGPDFQVELDG"
     gene            complement(2164..2973)
                     /locus_tag="PLK64_14135"
     CDS             complement(2164..2973)
                     /locus_tag="PLK64_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HPV80930.1"
                     /translation="MDKVVSSAAAALAGIESGMSLAVGGFGLCGIPSVLIQALHDSGV
                     DHLEVISNNCGVDDWGLGILLRDRRIRRMISSYVGENKEFERQYLSGELEVELTPQGT
                     LAERMRAGGSGIPAFFTMTGVGTQVAEGGMPWRYAADGTVAVASPAKEVREFDWMGRR
                     REFVLEQAIVADYALVRAARGDRHGNLVFKESTRNFNPLAAMAGRVTIAEVEQLVEPG
                     ELDPDQIHLPGIFVQRVLALSPEQASVKRIERRTVRPKPAAPSAAPTDQEA"
     gene            complement(3083..3937)
                     /locus_tag="PLK64_14140"
     CDS             complement(3083..3937)
                     /locus_tag="PLK64_14140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HPV80931.1"
                     /translation="MTASQSSPRVSVVVPTFHNAQFVEATIESILAQTFTDFELLVSD
                     HSSTDGTWDRLQRFAADPRVRLWQTPTGGGAPANWAAVTDAARGEFVKLVCGDDLLYP
                     TCLREQVDAMDAHPSATLVCARRDLIDAQGGIVMGGRGMSRIRGFHSGRAAIRRTVAL
                     GTNIFGEPASVLMRREALVGAGGWWPDFPYVIDEATYALVLLRGDLYAVPHSLAAFRI
                     SAGQWSVDLADQQAAQVIAFHEELDRRVPGLLTRADLARGKAMARGLSTARRLVYLAM
                     RRRMTTSE"
     gene            complement(3934..>4278)
                     /locus_tag="PLK64_14145"
     CDS             complement(3934..>4278)
                     /locus_tag="PLK64_14145"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HPV80932.1"
                     /translation="AVAPAWGSVLHNTVTLACSHVVSVIIAFVLYRTLVFRVTGHLWR
                     DLARFESVYLVSLAANWLLLNLLTVVIGLSVIVAQALVVAVIAMVSYFGHKHFSFRRP
                     DPAAPTPPGGAE"
BASE COUNT          675 a         1515 c         1470 g          618 t
ORIGIN      
        1 gtccagccga tggtgcgagc cacgtgatag tccccgaagc tcaccgcgtc ggcgtcgccc
       61 caggcccgtt gggccacctc ggcaaccgtc cacaccccga ccccggaaac cgatcgcagc
      121 cccgcccggc ccgcctcggt cgaccccgag gtgagccgct cgagggagcc ggcgacacgg
      181 catacccgct ggatcgtgtc cgagcgactg gcgtcgaccg acgcgcgcag gaactcccat
      241 gacgggatcg cccgccagcc cgcagcatcc ggtgggaccc gcagcccagc ggcgccccac
      301 ggccctggcg ccacatcgcc atggatccgg accagccggt ggtagccgcc gaacgcctcc
      361 ttgcccgtca ccttctgctc gatgatggcc ggcacgaggg cgtcgaggac caacccgctg
      421 cgcggcaccc gccaccccgg gaagcgccgg atcgcatcca cgacaggcgg gtgcaacccg
      481 acgaacccgc tcgggtcatc gccctcaccg accaacgccg gcgcctgctc caccgcccag
      541 gccgcgccag gcccccaagc tcgcgccgag atctcggaat cctttggggc acaattaagt
      601 tcgagggtgg ccggcccatc aggggtctgt atgccgcgca ccaccgaccc gtccggcccc
      661 acccggaacg tcgtgtcgcc accaccacga cggaacggcg cgaggatcgc cgcgagttgc
      721 acaggccggg cgctgcgcca ctgacgcacg actgcaggct cggccatgcc gcggactcta
      781 ccgacgtagg ctgacgctcg tgccgacccg atcctccatg cccgccgacg tcctcgccgc
      841 gatgttgcgc ttagacccgg ggcgccctcg catcacgtgt tacgacgatg cgagcggtga
      901 gcggatcgag ttgtccgcca agacctttgc caactgggtc gccaaggccg ccaacctctt
      961 gcaggacagc ggcgacgtca ccgtcggcaa caccgtcggc ctggacctgc ccgcccattg
     1021 gcgcacggcc tactgggcga tggcggcctg gtcggtgggc tgcatcgtcg tcgtcggccc
     1081 agaggcccgg gacgccgacg tcgtcgtcac cgtctccccc gaccttgccg ccgagtgcgt
     1141 caccgacggc cggtatgccg tgctcgtcac cctcgcggcg ctggcccgcg gcaacgcgca
     1201 gacgccgcag gacgtcctcg acgaggccaa ggaactggcc ggttatggcg accggttcga
     1261 cccgtgggac accccggagg cggacgagcc cgccctggtc ggcgacggtg acgacgcggc
     1321 atacgcgcag gtcgtcgtcc gtcgcgactg gccggagcgg ccacgggtcc tggtcagcgg
     1381 cgaccggctc gggtccgcgc tgcgagcgtt cctcagtgcc tgggccgccg acggatcggt
     1441 gctgctcgtg ctcaacccgg cgggcgatca ggaccgtcga cggacaatcg aaaatgtcac
     1501 tgccacagcc tgattcggtc agccgtcgag ctcgacctgg aagtcggggc cggtggcgga
     1561 gcgcacctcg tcgagagtga ccccgggcgc gagctcgcgc agaacgagcc cgtcgggggt
     1621 gacgtcgagg accgcgaggt cggtgatgat ccggtggacg acccgcttgc cggtgtaggg
     1681 cagcgagcac tcctgcacga tcttgtgggc accgtcgcga gcgacgtgct ccataaggac
     1741 gatgacgtgc tgggcgccgt ggacgaggtc catcgccccg cccatcccct tgaccatctt
     1801 gccggggatc atccagttgg cgatgtcgcc agccgccgac acctgcatgg cgccgaggat
     1861 cgccgcgttg accttgccgc cgcggatcat cccgaagctc gtcgcggagt cgaagaacga
     1921 cgctccacgc cggattgtca cggtctcctt accggcgttg atgaggtcgg ggtcctcctc
     1981 gccctcgaag ggataggcgc ccacgccgag gatgccgttc tcggactgca ggacgatctc
     2041 gacgtcgtcg gggacgtagt tgggcacgag ggtcggcagg ccgatgccga ggttgacgta
     2101 gtcgccgtcg tgcagctcgc gagcggctcg ggccgccatc tcctcacggg tgagcgccat
     2161 ggctcaggcc tcctggtcgg tcggtgctgc gcttggcgcg gcgggcttgg gtcgcacggt
     2221 gcggcgttcg atccgcttga ccgaggcctg ctcggggctc agggccagca cccgttggac
     2281 gaagatcccg ggcaggtgga tttggtcggg gtccaactcc cccggctcga cgagctgctc
     2341 gacctcggca atggtcaccc ggccggccat ggcagccagc gggttgaagt tgcgggtcga
     2401 ctccttgaag acaaggttgc cgtgccggtc gccgcgtgcg gctcggacga gggcgtaatc
     2461 ggcgacgatg gcttgctcga ggacgaactc gcgtcggcgc cccatccagt cgaactcacg
     2521 gacctccttg gccggcgagg cgacggcgac ggtgccgtcc gcggcatacc tccagggcat
     2581 gcccccctca gcgacctgcg tcccgacccc ggtcatcgtg aagaacgccg gtatgccgct
     2641 ccccccggct cgcatccgct cggccagcgt gccctggggg gtgagctcga cctcgagttc
     2701 gccggagagg tattgccgct cgaactcctt gttctcaccc acgtaggagg agatcatgcg
     2761 ccgaatccgc ctgtctcgca agaggattcc caacccccag tcgtcgactc cgcagttgtt
     2821 ggagatgacc tccaggtggt cgacgccgct gtcgtgcagg gcttggatga gcaccgaggg
     2881 gatcccgcag aggccgaacc cgccaacggc gagcgacatc cccgactcga tgccggcgag
     2941 ggcggcggca gcggaagaga cgactttgtc catgcccccg actctagccg cgcgtcgcca
     3001 gcggaatcag gccgggagcc ccagcacacg ttgtcggtgc gtggagttca cgcggtggaa
     3061 ccgcgccagc gcgcgcgttc ggctactcgc tcgtcgtcat ccggcgtcgc atcgcgagat
     3121 agaccagccg cctggcggtg ctcagtcccc tggccatcgc cttgccccgg gccaggtcgg
     3181 cacgggtgag cagacccggc acccgtcggt ccaactcttc gtggaacgcg atcacctggg
     3241 cagcctgttg atcggccagg tcgacactcc actgccctgc gctgatccgg aacgcagcca
     3301 ggctgtgggg cacggcatac aagtccccgc gcagcaggac cagcgcgtag gtggcctcgt
     3361 cgatgacgta ggggaagtct ggccaccagc cgcccgcgcc gacgagggcc tcgcgtcgca
     3421 tgaggaccga ggccggctcg ccgaagatgt tcgtgccgag ggcgaccgtg cgccggatcg
     3481 cggctcgccc actgtggaac ccacgaatgc ggctcatccc gcggcccccc atgacgatcc
     3541 cgccctgcgc gtcgatgagg tcgcgtcgcg cgcacacgag ggtcgcgctc gggtgggcgt
     3601 ccatcgcgtc gacctgctca cgcagacagg tgggatacag caggtcgtcg ccgcagacga
     3661 gcttgacgaa ctccccccgc gcggcgtcgg tgacggcagc ccagttggcc ggcgcaccgc
     3721 ctccggtcgg cgtctgccac agccgcaccc gagggtcggc ggcgaaccgc tgcagtcgat
     3781 cccaggtgcc gtccgtcgag ctgtgatcgc tcaccaacaa ctcgaagtcg gtgaaggtct
     3841 gggcgaggat cgactcgatc gtcgcctcca cgaactgcgc gttgtggaac gtggggacga
     3901 cgaccgagac ccgcgggctc gactgtgagg ccgtcactcc gcacctcccg gcggggtcgg
     3961 ggccgcgggg tcagggcggc ggaacgagaa gtgtttgtgc ccgaagtagc tcaccatggc
     4021 gatgacggcg acgaccaagg cctgcgcaac gatcaccgag aggccgatga cgacggtgag
     4081 gaggttgagc agcagccagt tggcggcgag cgacaccaga tagacgctct cgaaccgggc
     4141 caggtctcgc cagaggtgcc cggtgacgcg gaacacaagg gtgcggtaga gcacgaaggc
     4201 gatgatgacc gacaccacgt gggagcaggc cagcgtgacg gtgttgtgaa ggacgctgcc
     4261 ccaggcaggg gcgacggc
//
LOCUS       DAOMZY010000304         4246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_19680_length_4246_cov_6.071582, whole genome shotgun sequence.
ACCESSION   DAOMZY010000304 DAOMZY010000000
VERSION     DAOMZY010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19680_length_4246_cov_6.071582"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..1793
                     /locus_tag="PLK64_14150"
     CDS             213..1793
                     /locus_tag="PLK64_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HPV80933.1"
                     /translation="MTDATSSATPESAAPQPASSAPTQTPSATSANAAGAAPDGKVAE
                     PEPTDELVTTRHTVTLSDGQVLDYSATTGRLVLREEVYEDGVFGGHPAKAQVSITSYV
                     REGLSGRERAARPVTFAFNGGPGSSSVWLHLGLLGPRRVVMGDVGALLPPPYALADNA
                     ETLLAESDLVFIDPVSTGYSRAVKGGKPGVYHGYQGDLESVAEVIRLWTSRNGRWMSP
                     KFLAGESYGTLRAAALADHLQTRYGMYLNGVILISSVLDLSSIDFAEQRNDRAHAAYL
                     PLYAAVAHYHGKHPGRSLDEVVAEASEYAARDYPWALARGARLRADERAEVAATVARL
                     AGVSADYVDRAELRIEHWRFFGELLRDERLTVGRLDSRFTGPAASSIAEEMDADPSFD
                     AIVGPYAAAMNHYVRAELEFGSDLHYEQISERVNPWSYKDFEGRPIDVSPRLERAMRA
                     NPHLQVHVAYGIYDGATPPAAADDVFAVMRLTEEARGRIEHRTYPAGHMMYVHEPSRL
                     AQSADLASFVRRASGLSA"
     gene            complement(1872..2384)
                     /locus_tag="PLK64_14155"
     CDS             complement(1872..2384)
                     /locus_tag="PLK64_14155"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV80934.1"
                     /translation="MTIVVRPCESLADVAAIEASVPTGHSAYHAQRFHRNAEGAVYLL
                     AWRDDLAVGHVLVTPVSKYELVRARLGTFPEVNALGVAAAYRRCGVARTLMEAAAGLA
                     VSMGGTRLGLAVEPENQPALALYRALGFRRHPDVDPVDVWTWIDEAGVEHEERDQCTY
                     WTWPCQPGVG"
     gene            complement(2410..3381)
                     /locus_tag="PLK64_14160"
     CDS             complement(2410..3381)
                     /locus_tag="PLK64_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80935.1"
                     /translation="MTAGRTGDRPRVGDVAVPVDVTRPFTRRAGLATGLTDEQLQSTA
                     YRRIFRGVYADAATLVTAPARLEAARLIGPADAFATHHTAARLLGGIVPDTPVTHLGT
                     TSQRRGRVTDLRVHRYAALPPLVRIAGQLVTAPTQTFLDLAGSLDLVDLVVLGDSLVN
                     AGQIVPEDLRAASAASHTRWARSARRAAAYVRRGAESAPETRLRMLVVLAGLPEPQTQ
                     VPVLGSHGRVLYRLDMGWEEVKVALEYDGRHHIERQEKWHADLLRREDCESREWRFVV
                     ATSPDLYVGPHQTLGRVRTTLSDRGFVLPRLRDDWRVHFPSRETLTG"
     gene            complement(3821..>4246)
                     /locus_tag="PLK64_14165"
     CDS             complement(3821..>4246)
                     /locus_tag="PLK64_14165"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HPV80936.1"
                     /translation="RWDSLGEFSALGASLEHLAQTQGNAKAQVLAVALDKAIATFLDE
                     NKSPARKVGQIDNRGSHFYLALFWAEELAAQSDAAELAARFAPVAAALRSNESTINEE
                     LLAVQGKPVDMGGYYRPDTAKTGAAMRPSATLNAVIDAL"
BASE COUNT          688 a         1482 c         1437 g          639 t
ORIGIN      
        1 gtgcgcgatc gcgctggtcg atgccgcctc cgcgagctcc gtcgaggcgg cggtgctggc
       61 ggcgttcgcc ggggccgggt ggtctagccc ggttcccttc cttgtcacgc cggccgatgg
      121 agcagggcgc gaccagtaat gcggcgggac gggcgcgacc ggcaacccag cgtgacgggc
      181 cgacgtgaag cggccacggc tagggtgagg tgatgaccga cgcgacctcg tccgcgacgc
      241 ccgagtccgc ggcgccgcag cccgcctctt cggcacccac ccagactccc tcggccacga
      301 gcgccaacgc agccggggct gcaccggacg gcaaggttgc cgagcccgag ccgaccgacg
      361 aactggtgac gacacggcat accgtgacgc tctccgatgg gcaggtcctg gactacagcg
      421 cgacgaccgg gcgactggtg ttgcgcgagg aggtctacga ggacggcgtg ttcggcggcc
      481 acccggccaa ggcgcaggtg tcgatcacgt cctacgtccg cgaaggtctc tccgggcggg
      541 agcgggcggc gcggcccgtg acgttcgcgt tcaacggcgg gccagggtcg tcgtcggtgt
      601 ggctgcacct gggcctgctc gggccgcggc gggtggtcat gggcgatgtc ggtgccctgc
      661 tgccgccgcc gtatgccctg gccgacaacg ccgagacgct gctcgccgag agcgacctgg
      721 tgttcatcga cccggtgtcg accggctact cgcgggcggt caagggcggg aaacccggtg
      781 tctaccacgg ctatcagggc gaccttgaga gcgtcgccga ggtcatccgc ctgtggacct
      841 cgcgcaacgg ccggtggatg tcgcccaagt tcctcgccgg cgagtcctac ggcaccctgc
      901 gcgcggcggc actcgccgac cacctgcaga cccgctacgg catgtacctc aacggcgtca
      961 tcctcatctc gtcagtcttg gacctgtcgt caatcgactt cgccgagcag cgcaacgacc
     1021 gggcgcacgc ggcatacctc cccttgtatg ccgccgtcgc ccactatcac ggcaagcatc
     1081 ccggccgttc cctcgacgag gtggtcgccg aggcgagcga gtatgccgcc cgcgactacc
     1141 cgtgggcgct ggcgcgtggg gctcggctgc gcgccgacga acgtgcagag gtcgcggcga
     1201 cggtagcgcg gctcgcgggg gtgtcggccg actacgtcga tcgcgccgag ctgcggatcg
     1261 agcactggcg gttcttcggg gagttgttgc gggacgagcg gctgacggtc gggcggttgg
     1321 actcgcggtt caccgggccg gcggccagca gcatcgccga ggagatggac gcggacccgt
     1381 ccttcgacgc gatcgtcggg ccgtatgccg cggcgatgaa ccactacgtg cgcgctgagc
     1441 tggagttcgg cagcgacctg cactacgagc agatctccga gcgggtgaac ccgtggtcct
     1501 acaaggattt cgagggtcgg ccgatcgatg tctcgccacg gttggagcgg gcgatgcggg
     1561 ccaacccgca cctgcaggtg catgtggcct atgggatcta tgacggggcc actcccccgg
     1621 cggcggcgga tgacgtgttc gctgtgatgc ggctgaccga ggaggctcgt gggcggattg
     1681 agcaccggac ctatccggcc gggcacatga tgtacgtcca cgagcccagc cgcctagccc
     1741 agtccgccga cctggcgtca ttcgtccgcc gagcatctgg cctgtccgcc tgacttcccg
     1801 cgcctctgtc caccacaccc cggcctgttg acccggagat agcgctcgca cggtcatctt
     1861 tgccggccga gttaccccac tccaggctgg cagggccacg tccagtaagt gcactggtcc
     1921 cgttcttcgt gctccacgcc ggcttcatcg atccacgtcc acacgtcgac cggatcgacg
     1981 tccggatgcc gccggaaacc gagagcccga tacagggcca gtgcgggctg gttctcaggt
     2041 tcaacggcga gccccaaccg ggtgccgccc atggacaccg ccagccctgc cgccgcctcc
     2101 atgagtgtgc gggcaactcc acaacgccgg tatgccgcgg ccacgccgag cgcgttcacc
     2161 tccggaaacg ttccgagccg agcccgaacc agctcgtact tcgacaccgg agtgaccaac
     2221 acgtggccca cggccagatc atcacgccag gccagcaggt acacggcccc ttcggcgttc
     2281 cggtgaaagc gctgcgcgtg ataggccgag tgcccagtgg gcacggaagc ctcgatggcc
     2341 gcgacatcgg ccaaggactc gcacggcctc acgacgatcg tcaccgcaag agtctgcctg
     2401 atcagtcggt catccggtca gtgtttcccg gctcggaaag tgcacccgcc agtcatcccg
     2461 caggcgaggg aggacgaagc ctcggtcgct caacgtggtc ctcacccgcc ccagagtctg
     2521 gtgaggaccg acgtagaggt ccggcgacgt ggcgaccaca aacctccact cgcgcgactc
     2581 gcagtcttca cggcgcagca ggtccgcatg ccatttctcc tggcgttcga tgtggtgtcg
     2641 accgtcatat tcaagggcaa ctttgacctc ctcccacccc atgtcgaggc gatagagcac
     2701 ccgcccgtgg ctgccgagca ccggcacttg cgtctgcggc tcaggcaacc ccgccaacac
     2761 gacgagcatc cgcagccgag tctcaggcgc cgactccgct ccccgacgca cgtatgccgc
     2821 cgcgcgtcgt gccgaccgcg cccacctcgt gtgagaggcg gcgctcgcgg ctcgaaggtc
     2881 ctcggggacg atctgcccgg cattcaccag gctgtcgccg agcacgacga gatcgacgag
     2941 atccagcgac cccgccaggt cgagaaaggt ttgcgtcggc gcggtcacca gctgcccggc
     3001 gatgcggaca aggggcggga gggcggcata ccgatgcacg cggaggtcag tcacccgacc
     3061 gcggcgctgg ctcgtggtcc cgagatgcgt caccggggtg tcgggcacaa tcccacccag
     3121 caaacgggcg gccgtgtggt gcgtcgcgaa ggcgtccgcc ggtccgatga gccgagccgc
     3181 ctcaagccgg gcaggcgcgg tgacaagggt ggctgcgtcg gcatagacgc cgcggaagat
     3241 ccgccggtat gccgtgctct gcagctgttc gtcggtgagt cccgtcgcta accccgctcg
     3301 ccgggtgaac gggcgtgtga cgtcgacagg caccgcgaca tcgcccactc gtgggcgatc
     3361 gccggttcgt cccgctgtca tggaatgaag cctggcacgg ctcaagattc gccccccgag
     3421 gttatccaca ggcagcgatg ccgcttggct gggtccctct gatcgcgacc gacgacggtt
     3481 gggagccttg tccccggcaa agatgaccgt gcgagcgcta tcaccggacc aaaacccggg
     3541 gtcaccgccg caaagatgac catgcgagcg ctatctccgg gccaaacccc ggggtcaccg
     3601 ccgcaaagat gaccatgcga gcgctatccg ggtcaaatcg cccgccagac ccccaaagat
     3661 gaccgtgcga gcgctatcac cggaccaaaa cccagggtcg gggccccaaa gatgaccgtg
     3721 cgagcgctat ctccgggcca aaccccggga tggaaactca ggcgcaggta tgccgcccgc
     3781 gcggggtgcg cgggcggcat accaagacag cgggtgagga ctacagcgcg tcgatgaccg
     3841 cgttcagcgt tgcgctcggg cgcatggcgg caccggtctt ggcggtgtcg gggcggtagt
     3901 agccgcccat gtcgaccggc ttgccctgca cggcgagcaa ctcctcgttg atcgtcgact
     3961 cgttcgaccg cagcgcggcc gccaccggag cgaaccgagc cgccaactcg gcggcatctg
     4021 actgcgccgc caactcctcg gcccagaaca gcgcgaggta gaagtgcgag ccgcggttgt
     4081 cgatctgacc gaccttgcgg gccggcgact tgttctcgtc gaggaaggtc gcgatcgcct
     4141 tgtcgagcgc gaccgcaagc acctgagcct tggcgttgcc ctgggtctgg gccaggtgct
     4201 cgagcgaggc acccaacgcc gagaactcac cgagcgagtc ccaccg
//
LOCUS       DAOMZY010000305         4244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_19693_length_4244_cov_4.969205, whole genome shotgun sequence.
ACCESSION   DAOMZY010000305 DAOMZY010000000
VERSION     DAOMZY010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19693_length_4244_cov_4.969205"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLK64_14170"
     CDS             <1..206
                     /locus_tag="PLK64_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80937.1"
                     /translation="RATARLFSSASRAARASKQILRDSSGWPVAERFDLMREISLPVR
                     ASEDAREGARAFTEKRPPVWRGI"
     gene            727..1494
                     /locus_tag="PLK64_14175"
     CDS             727..1494
                     /locus_tag="PLK64_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HPV80938.1"
                     /translation="MASTTPSRPAAPARPPKAAMMVAQRIVRDSLRDGLKPGDLMSPE
                     RTMLEKYQTGRGTLREALRLLEFQGVIALKPGPRGGPVLQNPDASHLASTVVLLMQIN
                     EAPFRTIVEVRSAMEPMISSLAAVRMNEDDLADLAATIDQMRTDMDDQFSFLEANKRF
                     HDVIAWSSGNVLFGYIVDSLLGIMDGTVIGIHYPTHRRQAILVAHQEIYEALKSRDPA
                     ASQARMQEHIDAYVKYAERKYPQLLQETTPWDQRYFS"
     gene            complement(1599..2327)
                     /locus_tag="PLK64_14180"
     CDS             complement(1599..2327)
                     /locus_tag="PLK64_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV80939.1"
                     /translation="MLEITDLYAGYGQIEAVRGLSLSVDPGRVTLLLGANGAGKSTTL
                     RTVAGLHPPTSGTVVLNGVDVTGQPAHKMVRHGVSLVPEGRRVLAPMTVLENLRLGAY
                     TADKAAYARTLAEVNAMFPILADRSNGAAGLLSGGEQQMLAFGRALMSQPKIMLLDEP
                     SMGLAPAVTDRILANVRTMANSGIGILMVEQNAEAGLEVADHVIAVARGEVVFAGPAE
                     AARANASVLRAFLGEAALAGESGA"
     gene            complement(2320..3108)
                     /locus_tag="PLK64_14185"
     CDS             complement(2320..3108)
                     /locus_tag="PLK64_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPV80940.1"
                     /translation="MSAADATATRPVLEIRDLAVHFGGVKAVDRLSMRLDEGRIYGII
                     GPNGSGKSTLLGAVTRLTPLTSGEILFDGEPFHTVHASQIAAKRIARTFQTVRLLPDV
                     SVRDNIQLGADAARGDVDERRGWWARNFGTHTSPAVASAIARTRLGGFEDYRPAEMSY
                     GTQRRVEIARALALDPRLLLLDEPTAGMNQRERGEISTLLKELRDEGLTQLLVEHDVQ
                     MMVDTCDYVFAMNLGTVIAQGRPEDVVRDPQVQEAYLGKKFRHA"
     gene            complement(3105..4037)
                     /locus_tag="PLK64_14190"
     CDS             complement(3105..4037)
                     /locus_tag="PLK64_14190"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPV80941.1"
                     /translation="MSDFYAANLVLIQSTFIGILLALSVQVPMRAGVFSFAGAGSYGI
                     GAYVSAILVLKTGMPSLLAILISGIVAMVVGLLLGLLVAHLGGLYLAMATVSFDLIVS
                     VLAINGGDLTGGVTGLFGVLSDVTIFHLIGLALLGIVGVALTERGRVGRRVDAVRDDP
                     ELAQSVGISVRRYRLAAFVVSGLLGSVAGSLNSLVRSTISPSDIGFSLIVLALTMIIV
                     GGAQSWKGAVLGAVIFTWLPPLLAGFGQLQHVVYGVIVATAAIFVPGGLYGIYSEARY
                     RRTRAKRKTPQREAPAHEQALAISVPNGDAGRSA"
     gene            complement(4034..>4244)
                     /locus_tag="PLK64_14195"
     CDS             complement(4034..>4244)
                     /locus_tag="PLK64_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPV80942.1"
                     /translation="IKAFACIILGGVGSTGGVVLGSVLLAVTETVVLTRTSGLWVEAV
                     AFGLIFLVLLVKPSGLFGRKEVRRT"
BASE COUNT          708 a         1421 c         1482 g          633 t
ORIGIN      
        1 ggcgggcgac ggccaggctg ttttcgagcg cctcccgggc ggcgcgggcg agcaagcaga
       61 tcttgcgcga ctcgtcgggc tggccggtgg cggagcgttt cgatctgatg cgtgagatca
      121 gcctgccggt ccgggcctcc gaagacgctc gggagggtgc tcgcgccttc accgagaaac
      181 gccccccggt ctggcgcggc atctgagcca cattcgtcca ctcaggtgag tgaaaccgac
      241 gtcctgacgt cggtttcact cacctgaagg gtctgggcgc tcgctgccaa ggtgcttacg
      301 gcagtccgcc tcggcttccc ggagtgttgg ccacactctg gtgtggccaa cactccggga
      361 accactaggc cccgagcggc gggactggcc cgacgttgaa tcgcgtcctc acgggactga
      421 aaccgacgtt ctggtggcgg tcttgctcac ccgagggcga ctgagcagga ccaagaccgg
      481 gctgcgccca tctcgcgata cagcgcccgg gggaactcgc cagcgagctc caactcacga
      541 atctgcggac ggacgcgcgc gaggccttag tttctgactg cggcagccat ctcatcagcg
      601 gtatcgtcac cagttggtgc cactcgcgac aattcagaca ttgcggtgca cctcctcagc
      661 agccatctgg cgcgccctcg gccctttgct gttaatgttt accattgttc tgcagagagg
      721 catgagatgg ccagcaccac tccctcgcgg ccggcggcac cggcccggcc cccgaaggcc
      781 gccatgatgg tcgcccagcg catcgtccgc gacagcctgc gtgacggact caagcccggc
      841 gacctcatgt cgcccgagcg caccatgctt gagaagtacc agaccggccg tggcacgctg
      901 cgagaagcgt tgcggttgtt ggagtttcag ggagtcatcg ccctcaagcc gggcccccgt
      961 ggcgggccgg tcctgcagaa ccccgacgcc tctcacctgg ccagcaccgt cgttctgctc
     1021 atgcagatca acgaggcgcc gttccgcacg atcgtcgagg tccgctccgc gatggaaccg
     1081 atgatcagct cgctcgccgc cgtgcggatg aacgaggacg acctggccga cctcgccgcg
     1141 acgatcgacc agatgcgcac cgacatggac gaccaattct cgttcctcga ggccaacaag
     1201 cggttccacg acgtcatcgc ctggtcgtcg ggcaacgtgc tcttcggtta catcgtcgac
     1261 tcgctgctcg gaatcatgga cggcacggtc atcggcatcc actacccgac gcaccggcgc
     1321 caggcgatcc tcgtggccca ccaggagatc tacgaggctc tcaagtcccg cgacccggcg
     1381 gcgtcgcagg ctcggatgca ggagcacatc gacgcctacg tgaagtatgc cgagcggaag
     1441 tacccacagt tgctgcagga gacgactccc tgggaccagc gctacttcag ctgaccggct
     1501 ggggtgggcg ctgcccgccc cagcgccgat gactctgcgg cttgctgatc cactgccgcg
     1561 gcgcgtggcc gggcaggtca ggtcaggtca ggtcagggtc acgcgcccga ctcgccggcc
     1621 aacgccgcct cgccgaggaa ggcccgcagc accgaggcgt tcgcccgagc cgcttcggca
     1681 gggccggcaa agacgacctc accacgagca acggcgatga cgtggtcggc gacctccagg
     1741 cccgcctccg cgttctgctc caccatgagg atcccgatgc ccgagttggc catcgtgcgc
     1801 acgttcgcga ggatccggtc ggtgaccgcc ggcgccagcc ccatggacgg ctcgtccaga
     1861 agcatgatct tgggctggga catcagcgca cgaccgaagg cgagcatctg ctgctccccg
     1921 ccggagagca gcccggcggc gccgttgctg cggtcggcga ggatggggaa catcgcgttc
     1981 acctcggcaa gcgtgcgcgc gtatgccgcc ttgtcggctg tgtaggcgcc gagccgaagg
     2041 ttctccaaga ccgtcatggg cgcgaggacc cgacggcctt cgggcaccag cgagaccccg
     2101 tgtcgcacca tcttgtgcgc gggctggccg gtgacgtcga ccccgttgag cacgacggtg
     2161 cccgacgtgg gcgggtgcag gccggcgacg gtccgcaggg tggtcgactt gccggcgccg
     2221 ttggccccca gcagcagggt gacccggccc gggtcgacgc tcaggctcaa gccacgcacg
     2281 gcctcgatct ggccgtagcc ggcatacagg tcggtgatct caagcatggc ggaacttctt
     2341 cccgaggtag gcctcttgga cctgcgggtc gcggacgacg tcctccggcc ggccctgggc
     2401 gatgacggtg ccgaggttca tcgcgaagac gtagtcgcac gtgtcgacca tcatctgcac
     2461 gtcgtgttcg acgagcagct gggtgaggcc ctcgtcgcgc agttccttca gcagggtgga
     2521 gatctcgccg cgctcccgct ggttcatgcc ggcggtgggc tcgtcgagca gcaagaggcg
     2581 ggggtccagg gccagggcgc gggcgatctc cacccggcgc tgggtgccgt aagacatctc
     2641 cgctgggcgg tagtcctcga agccgccgag ccgggtgcgg gcgatggccg aggcgaccgc
     2701 gggggaggtg tgggtgccga agttgcgagc ccaccagccg cggcgttcgt cgacatcgcc
     2761 gcgggccgcg tcggcgccga gctggatgtt gtcgcggacg ctgacgtccg ggagcagccg
     2821 caccgtctgg aaggtccggg cgatccgctt ggccgcgatc tgcgaggcgt ggaccgtgtg
     2881 gaacggctcg ccgtcgaaga ggatctcccc cgaggtcagc ggcgtcagcc gggtgacggc
     2941 gcccaggagg gtgctcttgc ccgagccgtt cggtccgatg atcccgtaga tgcgaccctc
     3001 gtccaggcgc atcgacaacc ggtcgacggc cttgactccg ccgaagtgga ccgcgaggtc
     3061 gcggatctcc aggaccggcc gggtggccgt cgcgtctgcc gcgctcatgc cgacctccct
     3121 gcgtcgccgt tggggaccga gatggccagg gcttgttcgt gagccggtgc ctcgcgctgc
     3181 ggcgtctttc gcttggcgcg ggtgcgccgg taacgggcct cgctgtagat gccgtagagc
     3241 ccgccaggca cgaagatcgc cgcggtcgcc acgatgacgc catagacgac gtgctgcaac
     3301 tggccgaagc ccgccagcaa cggcggcaac caggtgaaga tgacggcccc gaggacggcg
     3361 cccttccacg actgggcgcc tccgacgatg atcatcgtca gggccaggac gatgaggctg
     3421 aagccgatgt ccgaggggga gatggtgctg cggaccagcg agttgaggga gccggccacc
     3481 gagccgagga gcccgctgac gacgaaggcc gccaaccggt agcgccgcac gctgatgccg
     3541 accgactgcg ccaactccgg gtcgtcgcgg accgcgtcga cccgtcggcc cacccggccg
     3601 cgctcggtga gggcgacccc gacgatgccc agcagggcca ggccgatcag gtggaagatc
     3661 gtcacgtcgg acagcactcc gaacaggccg gtgacgccgc cggtgaggtc gccaccattg
     3721 atcgcaagga ccgagacgat gaggtcgaag gacaccgtcg ccatggcgag gtagagccca
     3781 ccgaggtggg ccaccagcag cccgagcagg aggccgacca ccatcgcgac gatgccggag
     3841 atgaggatgg cgagcaggga cggcataccc gttttgagaa cgaggatggc cgagacgtag
     3901 gcgccaatgc cgtaggaccc tgcgccggca aaggagaaga ctcccgcgcg catggggacc
     3961 tggacgctga gcgcgagcag gatgccgatg aaggtcgact ggatgaggac gaggttggcc
     4021 gcgtagaagt cgctcatgtg cgacgcacct ccttgcggcc gaacagtcca gatggcttga
     4081 cgagcaggac gaggaagatg aggccgaagg ccaccgcttc gacccagagg ccgctcgtgc
     4141 gggtgaggac gaccgtctcg gtgacggcca gcaggaccga gccgaggacg acgccgccgg
     4201 tcgagccgac gccgccgagg atgatgcagg cgaaggcctt gatg
//
LOCUS       DAOMZY010000306         4222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_19838_length_4222_cov_8.424286, whole genome shotgun sequence.
ACCESSION   DAOMZY010000306 DAOMZY010000000
VERSION     DAOMZY010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19838_length_4222_cov_8.424286"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..1277
                     /locus_tag="PLK64_14200"
     CDS             177..1277
                     /locus_tag="PLK64_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPV80943.1"
                     /translation="MGLRVLPGGAAAYTLSTARDVEDFAQELVDQYALAMVAAGLSDG
                     HIRNSRAVVIEFARSMTGPLWEATPQHADQFLAEQRRLGRSVSTRAGKAGTLSAFYGF
                     VIDRYAGDIRRATGVQVEQPVDEYNRQSGASLGHVRVPPSDEEIDTLFSGWRESVPHA
                     RKYLPAARDYFAASLWRRLGLRITESVMLDIRDWRPDLGGLGKLHVRFGKGSRGRGPK
                     PRLVPAINGADRLIDWWLAEVRPQYGADWADPDAPMLPSERLDQQLGRCGRARADVLR
                     RTLNSQVDQWLPAWSGRMSPHVLRHYCASSLYGAGMDLKAIQELLGHLWLATTSGYIH
                     VRSDHIERAWGQANERLEDRFGQRRLSGPGTY"
     gene            1360..1722
                     /locus_tag="PLK64_14205"
     CDS             1360..1722
                     /locus_tag="PLK64_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011776830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPV80944.1"
                     /translation="MQWNLRLRAAERGIWKSAELRRMLAEAGLEISAGKMSSWWAGTP
                     ITMRLDDLDVLCAVLGCTTNDLMGLEPEKVASRRPRSAEAANGQDHAGHPGGEGGADP
                     RTPTVPPRLGTSRSRPPL"
     gene            2241..3563
                     /locus_tag="PLK64_14210"
     CDS             2241..3563
                     /locus_tag="PLK64_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80945.1"
                     /translation="MVFKRRVTPMPDYVTWTGRWSRKNKNQLPYRPGTGFDENVREQL
                     TLFDMAPDPEALRQRALVEDSDLTRYCAAIVADHAHRYGWSVRQRNTVMRTLRMLQAV
                     RPSPTAKIRASDIVALRRYDGTISSTIDVLAEAGLLIEDVPTRVEKYFTATFIADGAL
                     PQQMEQHLRLWLQVMLGGSRHSPRQVPRDPATVELHIRGLAPVVQCWAQAGHQSFAEI
                     TKGDILTALAALPQRTSHRHFAETGLRSLFKILKGRRLVFANPMRGIALTPVATNIPL
                     PLDTALIRAELNNPNPAVALAVALVAFHALTGKQVRELKLTDIVDGRLRLGSRDIPLG
                     APVRTRLTAWLDHRNTTWPASLNPHVLINRRTAPRLVPGGHAFPWKNSALRPQVLRED
                     RILHEIHATGGDLRRLCDLFGLSVAGATRYLSTIEHADLRFGEDTPAT"
     gene            3609..4043
                     /locus_tag="PLK64_14215"
     CDS             3609..4043
                     /locus_tag="PLK64_14215"
                     /inference="COORDINATES: protein motif:HMM:NF039954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5990 family protein"
                     /protein_id="HPV80946.1"
                     /translation="MLLEIRGHNLPGRSWHLEGRPCHNVHVGIQHGKEPVELVPGDSR
                     TALWETEIHVLERDSGIDFRGPTVQGRRGERFVYLTWGDVGDDGAFTMFARAKLMLHD
                     LEPLVSAEPPSQHVIATVDLTDECGGPRCARLRSPALELRAR"
BASE COUNT          700 a         1496 c         1390 g          636 t
ORIGIN      
        1 tcaggcggct atctctatgc atgacggttg gttccgagga tctgggaatg tgatccgcgt
       61 tcgtgcaggt ggtggtgctt gccaagctgg gagagattcg ttgatcattg gtcgtgtttc
      121 ctgacggttt cggtatatac tcggctcatg gtcggcatca gtgaactagc cttctgatgg
      181 gtctgcgcgt gctgccgggt ggcgcggccg cgtatacgtt gtcgacggcg cgggatgttg
      241 aggacttcgc gcaggagctt gtcgaccagt acgcgttggc gatggtggcc gcgggtttga
      301 gcgacgggca tatccgcaac agccgagctg tcgtgatcga gttcgccagg tccatgaccg
      361 ggccgttgtg ggaggccaca ccgcagcacg ccgaccagtt cctcgctgag caacggcgac
      421 tgggtcgcag tgtgtccacc cgtgccggca aggcgggcac gctgtcggcg ttctacgggt
      481 tcgtcatcga ccggtacgcc ggtgacatcc ggcgcgcgac cggtgtccag gtcgagcagc
      541 cggtcgacga gtacaaccgc cagtccggtg cctcgttggg gcacgtgcgg gtgccgccct
      601 cggacgagga gattgacacg ctgttcagcg ggtggcgcga gtcggtcccg cacgcacgca
      661 agtacctgcc ggcagcacgc gactacttcg cggcctcgct gtggcgtcgc ctcgggctgc
      721 ggatcaccga gtcggtgatg ctcgatatcc gcgactggcg ccccgacctg ggtgggctcg
      781 gcaagttgca cgtccgcttc ggcaagggca gccgcggccg cggaccgaag cctcgtctgg
      841 tgccggcgat caatggagct gaccggctca tcgactggtg gctggccgag gtccgcccgc
      901 agtacggggc ggactgggcc gaccccgacg cgccgatgtt gccctcggag cggctcgacc
      961 agcagttggg ccgctgcggt cgggcgcgtg cggacgtgct tcgcaggacg ctgaacagcc
     1021 aggtcgatca gtggcttccg gcgtggtcgg gccggatgag cccgcacgtg ctgaggcact
     1081 attgcgcctc ctcgctctat ggggccggga tggacctcaa ggcgatccag gagctcttgg
     1141 gccatctgtg gctggcgacg acctcgggct acatccacgt ccgcagcgac cacatcgaac
     1201 gagcgtgggg tcaggccaac gaacgactcg aggaccggtt cgggcagcga cgactgtccg
     1261 gcccgggcac ctactgacgc gaccgacaac ccgatcgacg acccgcacga acaactcgcc
     1321 aagcgcccca cgcaaggtcc gaaggaggag aggaagcaga tgcagtggaa cctgcggctg
     1381 cgcgccgccg aacgagggat ctggaagtcg gccgagctgc gacggatgct cgccgaggcc
     1441 gggctggaga tctccgcggg gaagatgtcc tcgtggtggg ccggcacccc gatcaccatg
     1501 cggctggatg acctggacgt gctgtgcgcg gtgctcgggt gcacgacgaa cgacctgatg
     1561 ggccttgagc cggagaaggt cgcctctcgt cgtccccgca gcgccgaggc tgccaacggc
     1621 caggaccacg ccggacaccc gggcggtgag ggtggagccg atcctcgcac gcccacggtg
     1681 ccgccacgac tggggacgtc gcgttcgagg ccgccgctgt gagggtgctg gcctggttgg
     1741 gttgaacagc gagcgagaca aggcacgcgg gcatgccggg accgggaccc aacgcgccgc
     1801 tgcggctgcc gccgaagtgc aatcgctgtc agctcaaccg tgtcgcgtgg accagccccc
     1861 gcgtcgacgt ctgctacgag tgccttcccg gcggcccgtt cgcagcgcct gcctgcgtgc
     1921 gctgcggcag ccaccaggac tacttcagcc agggcctgtg cggccgctgc catccccgca
     1981 gccccgaaca cgtcggctcc tgccggggct gtctggcctg gggtgtgtac ccgcgctaca
     2041 actggacctg ctggtcgtgc aggtggtggg aagcccacta ccccagaggc acgtgcgcac
     2101 actgcggccg tctgacgcac gttagcgaca cccaggcctg tcgcctctgc ttggagaacg
     2161 cccgccgcga acagagaccg gggcgcgccc ctgacgtagc cggtgcgaac cgacacggtc
     2221 agcagctgtt cttcgccaac atggtgttca agcggcgcgt caccccgatg cccgactacg
     2281 tcacctggac cggccgctgg tcgcgcaaga acaagaacca gttgccctac cggcccggca
     2341 ccgggttcga cgagaacgtt cgcgagcagc tgaccctgtt cgacatggcg cccgaccctg
     2401 aggcgctgcg ccagcgcgcc ctggtcgagg acagcgacct gacccgctac tgcgccgcca
     2461 tcgtggccga ccacgcccac cgctacggct ggagcgtgcg acagcgcaac acggtgatgc
     2521 ggaccctgcg gatgctgcag gccgtgcggc ccagcccgac cgccaagatc cgggccagcg
     2581 acatcgtggc gttgcgccgc tacgacggca cgatctcctc caccatcgac gtcctggccg
     2641 aggccggcct gctcatcgag gacgtcccca cccgggtgga gaagtacttc accgcgacgt
     2701 tcatcgccga cggcgccctg ccgcagcaga tggaacagca cctgcgcctg tggctgcagg
     2761 tcatgctcgg cggctcgcgc cactcgcccc gccaggtccc acgcgacccg gccacagtcg
     2821 agctgcacat ccgcggcctc gcgccggtgg tgcagtgctg ggcccaggcc ggacaccagt
     2881 ccttcgccga gatcaccaag ggcgacatcc tcacggccct ggccgcattg ccgcaaagga
     2941 ccagccaccg gcacttcgcc gagaccgggt tgagatcgct gttcaagatc ctcaagggac
     3001 gccgcctcgt gttcgccaac cccatgcgag gcatcgccct caccccggtc gccactaaca
     3061 tcccgctgcc gctggacacc gccctgatcc gcgccgagct gaacaacccc aaccctgcgg
     3121 tcgcgctggc agtcgcactg gtcgccttcc acgccctgac cggcaagcag gtccgcgagc
     3181 tgaagctgac cgacatcgtc gatggacgcc tgcgcctcgg tagccgggac atcccgctcg
     3241 gcgcgcccgt gcggacacgg ctcaccgcct ggctcgacca ccgcaacacc acctggcctg
     3301 ccagcctcaa cccgcacgtg ttgatcaacc gacgcaccgc accccggctc gttcccggtg
     3361 gtcacgcctt cccctggaag aacagcgcgc tgcgccccca ggtcctgcgt gaggaccgca
     3421 tcctgcacga gatccacgcc accggaggag acctgcggcg cctctgcgac ctgttcggcc
     3481 tcagcgtcgc cggagcaacc cgctacctga gcaccatcga gcacgctgac ctgcgattcg
     3541 gcgaggacac acccgcaacc tgagccaacc tcagggtcgc aacgcgcccg cgctggcatc
     3601 atggtcgtgt gctgctcgag atccgtggac acaacctccc cggccggtcc tggcacctcg
     3661 agggccggcc gtgccacaac gtccatgtcg gcatccagca cggcaaagag cccgttgaac
     3721 tggttcccgg cgacagccgg accgctctct gggagacgga gatccacgtc ctcgaacgcg
     3781 actcgggcat cgacttccga gggccgacag tgcaaggccg ccgcggagaa cgattcgtct
     3841 atctgacttg gggcgacgtc ggcgacgacg gcgcattcac gatgttcgcc cgcgccaagc
     3901 tgatgctcca cgacctcgaa ccgctggtat cggccgaacc gccgagccaa cacgtcatcg
     3961 ccacagtcga cctcaccgac gagtgcgggg gaccgcgatg cgcgcgactt cgttcgcccg
     4021 cgctggagct tcgcgcccgc tgatcctgcg tcgtcagccc cgaaggggcc gcccccgtcc
     4081 agcagaacgt caagaacccc gccccggttg gcaacggcca ggcaattcgg cgaacatgac
     4141 gacacacacc ccgccaaccc gccgaacggg tgcaagttcg ccgagaaacc gtcagaaatc
     4201 acgcgaacag tcatagggcg cc
//
LOCUS       DAOMZY010000307         4158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_20255_length_4158_cov_5.526688, whole genome shotgun sequence.
ACCESSION   DAOMZY010000307 DAOMZY010000000
VERSION     DAOMZY010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20255_length_4158_cov_5.526688"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..1129
                     /gene="ccsB"
                     /locus_tag="PLK64_14220"
     CDS             44..1129
                     /gene="ccsB"
                     /locus_tag="PLK64_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HPV80947.1"
                     /translation="MPFSTALPAVAIVGAAPSPELASAANLALYSAMAVFTIAMLLYT
                     AYLAALGPVSRAGQAGERAKELVAVGADATGASSGSSGSGGVVGSADGAAAAVDADDA
                     DDAAVAGPSLRARKLAGSAYGMTCLATLVLVGSVVLRGLAVMRPPWGNMFEFATAGAT
                     AAAVAYCLLARRNRWEWLGLFVVGPVLLTLGTALLAFYTEAAELMPALKSVWLIIHVT
                     VAILAVGVFVLGFSVGVVYLVKSRREATTPDRRTFWETLPSAASLERTAYGLNMVGFI
                     LWTFTLVAGAIWAQKAWGSYWTWDPKEVWTFVVWVVYAAYMHARATAGWEPRKAMWIA
                     IAGFVTVVLNFAVVNVYFVGQHSYSGL"
     gene            1160..1513
                     /locus_tag="PLK64_14225"
     CDS             1160..1513
                     /locus_tag="PLK64_14225"
                     /inference="COORDINATES: protein motif:HMM:NF025378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4229 domain-containing protein"
                     /protein_id="HPV80948.1"
                     /translation="MLKYTVVRLGLFAICFGIVWAVWLRGLDSDFGSLIRSYPDQMFW
                     GLVIAAVSSMAVSFFALRQMREDISTGINERVNRRLSAKGADTDPASSEGAQRRRSAA
                     DDDADAEDAEADSQP"
     gene            complement(1601..2479)
                     /locus_tag="PLK64_14230"
     CDS             complement(1601..2479)
                     /locus_tag="PLK64_14230"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HPV80949.1"
                     /translation="MATLAQWVAGARPRTLPAAVSPVLVGTGAAYAVGRGDAGLAVLA
                     LAVALSLQVAVNYANDYSDGVRGTDAERVGPVRLVGQGLAVPGEVKLAAYLGFFLAAL
                     FGLALVALSQIWIFIPIGIACIIAAWRYTGGEHPYGYRGLGEVMVFVFFGLVATLGTQ
                     VTQAGGLTWAGLLGAIGIGCLASAILVANNLRDIPSDTVAGKHTLAVRLGDARTRRLW
                     TGLVGVAFAVAVGCAVWRVGSLVALLAVGLAVGPWRTLRSGAVGRALVPVLVATGRLQ
                     LGYAVLLALGLALTPR"
     gene            complement(2602..3792)
                     /gene="menE"
                     /locus_tag="PLK64_14235"
     CDS             complement(2602..3792)
                     /gene="menE"
                     /locus_tag="PLK64_14235"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HPV80950.1"
                     /translation="MRLQPIPLPFGPALLAAVGPLAAVLDGSGPPLLPYAAGSPPPVL
                     PPVTDADLPDDLALVVSTSGSTGIPKRTMLTANNLLASASAAHDVLSGPGSWLLALPA
                     HHIAGLQVIVRAALGSAGEPAVMDLTDGFTVEGFVAAALAMPPTSDPTYVSLVPTQVA
                     RLLADEAGIETLAAFDAVLCGAAAIPAAVRTRAHDLGIHLVTTFGMTETSGGCVYDGK
                     PLPVSEVHIDNDRHVVLGGATVAAGYLGDPGMTADHFTDDADGVRWFRAEDLGYLDDN
                     GRLHVTGRADEIINTGGVKVAPGPVEDALVRYLPGVRDAVVVGVPDPTWGQVVAAAVT
                     LLPSAGSARSAGVPTVEDARAALRGILPDAALPRLLQVVDAFPAKGPGKPDRAAISAS
                     FAPS"
     gene            complement(3806..>4158)
                     /locus_tag="PLK64_14240"
     CDS             complement(3806..>4158)
                     /locus_tag="PLK64_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPV80951.1"
                     /translation="YLAKMVGQKFAREIFFLGRAYTAEDMHRMGAVNLVADHAEVEAV
                     ALQVAAEINGKSPTAQRMLKFAFNLLDDGLMGQQVFAGEATRLAYMTDEAVEGRDSFL
                     QKRDPDWSAFPWYF"
BASE COUNT          623 a         1384 c         1538 g          613 t
ORIGIN      
        1 cctccggtgc cgcccccgcg gcccgtcccg aaaggacgtg agtatgccgt tctccacggc
       61 cctgcccgcc gtcgcgatcg tcggtgcggc accgtcgccg gagttggcca gcgccgccaa
      121 cctcgcgctc tactcggcga tggccgtgtt caccatcgcg atgctgctct acacggcata
      181 cctcgcggcg ttggggccgg tgtcccgggc ggggcaggcc ggcgagcggg ccaaggagct
      241 cgtggccgtc ggtgccgacg cgacgggcgc gtccagcggt tccagcggtt cgggtggtgt
      301 tgtcggtagt gctgatggcg cggcagcggc ggtcgacgcg gacgatgcgg acgatgccgc
      361 cgtggccggg ccgtcgctgc gggcccggaa gttggccggc agcgcctacg gcatgacctg
      421 cctggccacc ctcgtgctgg tggggtcggt tgtcctgcgc gggctggccg tcatgcggcc
      481 gccgtggggc aacatgtttg agtttgcgac cgccggggcg accgcggccg ccgtcgccta
      541 ctgtctgctg gcccgacgca accggtggga gtggctcggc ctcttcgtcg tcgggccggt
      601 gctgttgacc ctcggcacgg cgctgctggc cttctacacc gaggcggccg agctgatgcc
      661 ggccctcaag agcgtctggc tgatcatcca cgtcaccgtc gccatcctcg cggtcggggt
      721 gttcgtcctc gggttttcgg tgggtgtcgt ctatctggtc aagtcccgcc gggaagcgac
      781 gaccccggac cggcgcacct tctgggagac gctgccgtcg gccgcgtcac tggagcgcac
      841 ggcatacggg ctcaacatgg tgggcttcat cctgtggacc ttcacgctcg tggccggcgc
      901 gatctgggcg cagaaggcgt ggggctccta ctggacgtgg gaccccaagg aggtctggac
      961 gttcgtcgtg tgggtggtgt atgccgccta catgcacgcc cgcgcgaccg ccggttggga
     1021 gccgcgcaag gcgatgtgga ttgccatcgc cggcttcgtc accgtggtgc tcaacttcgc
     1081 ggtcgtcaac gtctatttcg tcggccagca ctcctattcg ggtctgtgag cctgatcgcg
     1141 ccgtgtcgag agggtcgtcg tgctgaagta caccgtggtg cgcctcggat tgtttgccat
     1201 ctgcttcggg atcgtctggg cggtgtggtt gcgcgggttg gacagcgatt ttggatcgct
     1261 cattcgcagc tatccggatc agatgttctg ggggctggtc atcgccgcag tgtcctcgat
     1321 ggcggtgtcc ttcttcgcgc tgcgccagat gcgtgaggac atctcgactg gcatcaacga
     1381 gcgggtcaat cggcggttga gtgccaaggg cgcggacacc gacccggcca gctccgaggg
     1441 cgctcagcgc cgccgctcgg ctgccgacga cgacgccgat gccgaggacg ccgaagccga
     1501 ctcccaaccc tgatcgcctc ccgccccctc tgccgggtcg aggtattccg gcatcgtctg
     1561 gtaacggcgg aatacctcga cccggcaggg gtcagcgggg tcagcgaggg gtgagggcca
     1621 ggccgagagc cagcaggacg gcatacccca attgcaggcg tccggtcgcg accaggacgg
     1681 gcacgagcgc ccgcccaacc gcacccgagc gcagcgtccg ccacgggccg accgcgagcc
     1741 cgacggccag cagcgcgacg agcgacccca cccgccagac cgcgcacccg acggcgaccg
     1801 caaacgcgac gcccacgagc cccgtccaca gccgacgcgt ccgggcatca cccagccgca
     1861 ccgcgagcgt gtgtttgccg gccaccgtgt cggaggggat gtcccgcagg ttgttggcga
     1921 cgaggatggc cgacgccagg cacccgatgc cgatcgcgcc gagcaacccg gcccacgtga
     1981 ggccgcccgc ctgggtgacc tgcgtcccga gggttgcgac caacccgaag aagacgaaca
     2041 ccatgacctc gccgagcccg cgatagccgt aggggtgctc gccgccggtg tagcgccagg
     2101 ccgcgatgat gcaggcgatg ccgatgggga tgaagatcca gatctgtgac agggcgacga
     2161 gggccagccc gaagagcgcg gccaggaaga acccgaggta tgccgcgagc ttcacttcgc
     2221 cgggaacagc cagtccctgc ccgaccaggc gcaccggccc gacccgctcg gcgtcggtgc
     2281 cgcggactcc gtcggaatag tcgttggcgt agttgaccgc tacctgtagg gacaacgcca
     2341 cggccagcgc gagcacggcg aggccggcgt caccccgccc aacggcatac gccgcgccgg
     2401 tgcccacgag gacgggggag acggcggcgg ggagtgtgcg cgggcgggca ccggcgaccc
     2461 attgggcgag cgtggccatg acggtgagaa gtccttcgga gcgtgaggcg agtgggtggt
     2521 gaacctgctg ttcccgcggc gtcgatgcgg cgggtggcga gcggcgaaag gcgcagtgtg
     2581 cgcctccacc ctacgggcgg ctcacgacgg cgcgaaggag gcgctgatcg cggcgcggtc
     2641 gggtttgcct ggacccttgg ccgggaaagc gtcgacaacc tgcaacagtc ggggcagggc
     2701 ggcgtcgggg aggatgccac gcaaggccgc ccgggcgtcc tccaccgtgg ggacgccagc
     2761 cgagcgtgca cttccggccg agggcagcag ggtgaccgcg gcggcgacga cctggcccca
     2821 cgtcggatcg gggacaccga caacgacggc atcgcgcacg cccggcaggt agcgcacgag
     2881 ggcgtcctcg accgggcccg gggcgacctt gacgccaccc gtgttgatga tctcgtcggc
     2941 tcgaccggtc acatgaagtc ggccgttgtc gtcgagatag cccaggtcct cggcgcggaa
     3001 ccaccgcacc ccatcggcgt cgtcggtgaa gtggtcggcg gtcatgccgg ggtcaccgag
     3061 atagccggcc gcgaccgtcg cgccgccgag gacgacgtgg cggtcgttgt cgatatggac
     3121 ctcgctgacc ggcagcggct tcccgtcata gacgcaaccg cccgaggtct cggtcatccc
     3181 gaacgtcgtc accaggtgga tgccgaggtc gtgggcgcgg gtgcgcaccg cagccgggat
     3241 cgccgcagcc ccgcagagga cggcgtcgaa ggcggccagg gtctcgatgc cggcttcgtc
     3301 ggctagcaac cgagccacct gggtcgggac gagcgacaca taggtcgggt cggaggtggg
     3361 gggcatcgcg agcgcagcgg cgacgaaacc ctcgaccgtg aaaccgtcgg tcagatccat
     3421 cacggccggt tcacccgcgg accccaacgc ggcccggacg atgacttgca ggccggcgat
     3481 gtggtgggcg ggcagggcca gcagccatga gccaggcccg gacaggacgt cgtgcgccgc
     3541 gctggccgat gcgagcaggt tgttggcggt gagcatggtc cgcttgggga tccccgtcga
     3601 ccccgacgtc gacacgacga gcgcgaggtc gtcgggaagg tccgcgtccg tcaccggcgg
     3661 cagcaccggg ggaggtgagc ccgcggcata cgggagcagc ggtggccccg acccgtcgag
     3721 gacggccgcg agggggccta ccgcagccag gagcgcgggt ccgaaaggca gggggatcgg
     3781 ctgcaatcgc atgaggtatg ccgggtcaga agtaccacgg gaaggcggac cagtcggggt
     3841 cgcgcttttg caggaaggag tcgcggcctt cgacggcctc gtcggtcatg taggccagcc
     3901 gggtggcctc gccggcgaac acctgctgtc ccatgagccc gtcgtcgagc aggttgaagg
     3961 cgaacttgag catccgctgg gcggtcggcg acttgccgtt gatctcggcg gcgacctgca
     4021 gcgcgacagc ctcgacctcg gcgtggtcgg cgaccaggtt gaccgcgccc atccggtgca
     4081 tgtcctcagc ggtataggca cgaccgagga agaagatctc gcgggcgaac ttctggccga
     4141 ccatcttggc gaggtagg
//
LOCUS       DAOMZY010000308         4154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_20285_length_4154_cov_4.351305, whole genome shotgun sequence.
ACCESSION   DAOMZY010000308 DAOMZY010000000
VERSION     DAOMZY010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20285_length_4154_cov_4.351305"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..981
                     /locus_tag="PLK64_14245"
     CDS             79..981
                     /locus_tag="PLK64_14245"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPV80952.1"
                     /translation="MTKKQSRVQSFSDAVVVGVDGHDSTHAALLWAAAEAGRRKTRLV
                     VMTIQEAYTHDLPFGAGVDEATAIADADTDVTARLAAQAARDAVPGLKVSTVRPEGQA
                     ASRLIKASKSAALIVLGSHGRHKRAYAALGTTASTTAMHAKCPVVVVHPDRSAAHAAG
                     PAHVVVGVDGSRDSSLAIDAAADLAGKDGHVNVVNAWWFSMAESGSLVGGDVVEEDKI
                     RARHARGVRNIAAAAQKRHPKVTFTAVSVEGEAVAALVEQAKEADLLVVGARGRGGFG
                     GLLLGSNALKALAASPVPVAVIHK"
     gene            complement(1037..1867)
                     /locus_tag="PLK64_14250"
     CDS             complement(1037..1867)
                     /locus_tag="PLK64_14250"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPV80953.1"
                     /translation="MDDYLDINRANWDERAPAHAVSEGYAVRRLVSDPAALSGVVEFD
                     RPLLGDIAGLRGVHLQCHIGTDTLSLHRLGARMSGLDFSPAALHEARALAAEAGAEID
                     YVESTVDGAVAAFGAGGFDLVYTGIGALCWLPRIRDWAQTVATLLRPGGRLFIREGHP
                     MLWAMADPTGPESPVAVGYTYFEQPDAVVWDEPGTYVETEVEFKHNVTHSWNHGLGEI
                     VTALLDVGMVITGLVEHDSVPWEALPGLMVLDERGEWRLRENPERLAASYTLQAVKAR
                     "
     gene            complement(1917..2801)
                     /locus_tag="PLK64_14255"
     CDS             complement(1917..2801)
                     /locus_tag="PLK64_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HPV80954.1"
                     /translation="MPEGHTLHRLAGSLNGAFAGTLPTASSPQGRFAEGAAYLTGREL
                     HEAAAWGKHLFVEFEGAAYLHVHLGLIGTFDVTPFGLDESDAAPPPPVGAVRLRLATP
                     THVADLRGATVCELATPDQVDAVIARLGPDPLRADADPDRAWRRIAKSPRSIADLLMD
                     QAVLAGVGNVYRSEILFRHRVDPHRPGREIRPQTWKALWRDLLLLMPIGVQLGQIVTM
                     SDQVDAVLAGDFDPSAVPTSPTGVSAWRPNPHGALERRYYVYKRQGEACRVCGSRVRM
                     ADVAGRNLYWCGRCQRRR"
     assembly_gap    2839..2938
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2939..3849)
                     /locus_tag="PLK64_14260"
     CDS             complement(<2939..3849)
                     /locus_tag="PLK64_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HPV80955.1"
                     /translation="MRAMWKGAVSFGLVNVPVKLYSATENHDISFRQVHRADGGQIRY
                     QRVCSVCGDVVAFDDVAKGYETPDGQMVVLTDEDLAALPNQSSKEVAVAKFVPAGQID
                     PMLFDKPYYLEPEKAATKAYALLRDALLAADRVALVTVSIRQRMTMAVLRVHDDVIVL
                     QTLMWPDEIRAADQVNLGDTGHASDKELAMASMLVDSLSGDYDPAEFEDDYAGAVAAL
                     VDSKVTGAAVIEAPAAAADTGEVVDLLEALQRSVDRAKAARGEPVTPAVPAASAAAAP
                     AQRAAAAKTTPAKTAATKATAAKTAAA"
BASE COUNT          610 a         1463 c         1373 g          608 t
ORIGIN      
        1 acgttccgct cgtcacatct gagccgatca ggccctcaga cccagttgtc agatcactga
       61 caatcggata atggctgcat gacgaagaaa cagtccagag tccagtcgtt ctccgatgct
      121 gtcgtggtcg gcgtggatgg ccacgacagc acgcatgccg cgctgctgtg ggcggccgcg
      181 gaggctggcc gtcgcaagac ccgtttggtg gtcatgacga tccaggaggc ctacacccac
      241 gacctgccct tcggagcagg ggtcgatgag gccaccgcga tcgccgacgc cgacaccgat
      301 gtcacggcac gcctggccgc ccaggccgca cgcgacgccg ttccgggcct caaggtgagc
      361 accgtgcgcc ccgagggcca ggccgccagc cgcctcatca aggcctccaa gtcggccgcc
      421 ctgatcgtgc tcggaagcca cggcagacac aagcgggcgt atgccgcgct cggcaccacc
      481 gcctccacca cggcgatgca cgccaagtgc ccggtcgtcg tcgttcaccc cgaccggtcc
      541 gccgcccacg cggccgggcc ggcgcatgtc gtcgtcgggg tcgacggctc gcgtgactcc
      601 tcgctggcca tcgacgccgc cgcggacctg gccggcaagg acggccacgt caacgtcgtc
      661 aacgcctggt ggttcagcat ggctgagagc ggctccctcg tcggcggtga cgtggtcgag
      721 gaggacaaga tccgggctcg gcacgctcgt ggggtccgca acatcgccgc cgctgcccag
      781 aagcgtcacc ccaaggtgac cttcaccgcg gtgtcggtcg agggcgaggc cgtggcggcg
      841 ctcgtcgagc aggccaagga ggccgacctc ctcgtcgtcg gagcccgtgg gcgcggcggc
      901 ttcggcgggc tgctcctcgg gtccaacgcc ctcaaagccc tggctgcctc ccccgtgccc
      961 gtcgcggtga tccacaagta ggcatatgga cgaggtgggg tggtgcctgc gggggcgcca
     1021 ccccaccgcc gttccgtcag cgtgccttga cggcctgcaa cgtgtaactg gcggccagac
     1081 gctcggggtt ctcccgcagc cgccactcgc cgcgctcgtc gaggaccatg aggccgggca
     1141 gcgcctccca cggaacgctg tcgtgctcca cgagcccggt gatcaccatc ccgacgtcca
     1201 acagcgccgt gacaatctcg ccgagcccgt ggttccacga gtgggtgacg ttgtgcttga
     1261 actcgacctc ggtctcgaca taggtcccgg gctcgtccca caccaccgcg tcgggctgct
     1321 cgaagtaggt gtaaccgacc gccacggggc tctccggccc ggtcgggtcg gccatcgccc
     1381 acagcatcgg gtggccctcg cggatgaaca gccgaccgcc tgggcgcagc agggtcgcca
     1441 ccgtctgggc ccagtcgcgg atccgcggca gccagcacag cgccccgatg ccggtataga
     1501 ccaggtcgaa gcctccggcg ccaaacgcgg ccaccgcccc gtcgaccgtc gactcgacat
     1561 agtcgatctc ggcgcccgcc tcggcggcca gggccctcgc ctcgtggagg gctgcgggtg
     1621 agaagtccaa gccgctcatc cgggccccga gtcggtgcag ggacagcgtg tcggtgccga
     1681 tgtggcactg caggtggact ccgcgcaaac ccgcgatgtc gcccagcagc gggcggtcga
     1741 actcgaccac gccggagagg gcggccgggt cgctgaccag ccggcgcacg gcatacccct
     1801 cggagaccgc gtgtgcggga gcgcgctcgt cccagttcgc gcggttgatg tccaggtagt
     1861 cgtccatggt cgccgagtct aggtatgccg cccgcgggct cagcgacgat tacgactcag
     1921 cggcggcgtt ggcagcggcc acaccagtag aggttgcgac cggccacgtc ggccatccgc
     1981 acccgagagc cacagacccg acacgcctcg ccctgacgct tgtagacgta gtagcgccgc
     2041 tcgagcgccc cgtgcgggtt gggacgccaa gccgacaccc cggtgggcga ggtcggcacc
     2101 gcgctcgggt cgaagtctcc cgccagcacc gcgtcgacct gatcgctcat cgtgacgatc
     2161 tggccgagtt ggacaccgat gggcatgagc agcaacagat cgcgccacag ggccttccac
     2221 gtctgcggcc ggatctcgcg gccgggccgg tgggggtcga cgcgatgccg gaagaggatc
     2281 tcgctgcgat agacgttgcc cacgccggcg aggaccgcct ggtccatgag caggtcggcg
     2341 atgctgcgcg ggctcttggc gatccgtcgc caggcccggt ctgggtcagc gtcggcccgc
     2401 aacgggtcag gcccgagccg ggcgatgacc gcgtcgacct gatcgggcgt ggccagctcg
     2461 cacacggttg cgccgcgcag gtccgcgaca tgggtcggcg tggccaatcg cagccggacc
     2521 gcacccacgg gcggcggcgg cgcggcgtcc gactcgtcca ggccgaacgg ggtcacgtcg
     2581 aaggtgccaa tgagaccgag atgcacatgc aggtaggccg cgccctcgaa ctcgacgaac
     2641 aggtgcttcc cccacgccgc cgcctcgtgc aactcgcgac cggtgaggta tgccgctccc
     2701 tcggcgaagc gtccctgcgg gctcgacgcc gtgggcagag tgccagcgaa cgcaccgttg
     2761 agtgaccccg ccagccggtg cagggtatgg ccttcaggca tccggtcagc cggccttgcg
     2821 cgtcgcggtc ttcttggtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt
     2941 ggctgccgct gttttggccg ccgtcgcctt ggtggcagcg gtcttcgctg gcgtggtctt
     3001 ggctgccgca gccctctgcg ccggcgctgc ggcggccgag gcggccggaa cggccggggt
     3061 taccggctcg ccgcgagcgg ccttggctcg gtcgacgctg cgctgtaggg cttccaggag
     3121 gtcgacgacc tcgcccgtgt cggcagcagc cgccggggcc tcgatcacgg cggcgcctgt
     3181 caccttggag tcgaccaggg cggccaccgc cccggcgtag tcgtcctcga actcggccgg
     3241 gtcgtagtcg ccggacagcg agtccaccag catgctcgcc atcgccagtt ccttgtcgct
     3301 ggcatgcccg gtgtcgccga ggttgacctg gtcggccgcc cggatctcgt cgggccacat
     3361 gagggtctgc aggacgatga cgtcgtcgtg gacccgcagc accgccatgg tcatccgctg
     3421 acggatcgac acggtcacca gggccacccg gtcggcggcc agcagcgcgt cgcgcaacag
     3481 cgcatacgcc ttggtggcgg ccttctcggg ctcgaggtaa tacggcttgt cgaagagcat
     3541 cgggtcgatc tgaccggccg gcacgaactt ggcgaccgcg acctccttgc tcgactggtt
     3601 gggcagcgcg gccaggtcct cgtcggtgag cacgaccatc tgcccgtcgg gcgtctcgta
     3661 gcccttggcg acgtcgtcga aggccacgac gtcaccgcaa acgctgcaca cccgctggta
     3721 gcggatctgc ccgccatcgg cccggtggac ctggcggaag gagatgtcat ggttctcggt
     3781 cgccgagtag agcttgacgg gcacattgac cagcccgaaa gacaccgcgc ccttccacat
     3841 cgcccgcatc gaaccccacc cttccccgtg gccacgtacg tcaccagcat ggctcaccgc
     3901 tcgcctcccc acaacgccga cgtccatcgg gtatgccgcc cgtccacctc ccgatcaccc
     3961 gccgggacgc tgccgtttgc ccaaggacgg cataatcgcc cctgtggtga tgacgtggac
     4021 ggcgcgtgcg ctgaccgagg aggagcacga ggtcgtgcgc tccaatggca aggccgccgc
     4081 gaccctcatc gaggacgaac ctcctgatcc caagcgcggt gtcgatctcc acaccgcacg
     4141 gcatggactc cact
//
LOCUS       DAOMZY010000309         4119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_20490_length_4119_cov_4.367864, whole genome shotgun sequence.
ACCESSION   DAOMZY010000309 DAOMZY010000000
VERSION     DAOMZY010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20490_length_4119_cov_4.367864"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /gene="nadA"
                     /locus_tag="PLK64_14265"
     CDS             complement(<1..1103)
                     /gene="nadA"
                     /locus_tag="PLK64_14265"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HPV80956.1"
                     /translation="MSIETVTPKHTPLGLLVLGQGRDLHSERGVECPGDLPAPSDPDL
                     VRRARAAKAALGDKVFVLGHHYQRDEVIEFADVTGDSFKLAQQAAARPEAEWIVFCGV
                     HFMAESADILTTDAQTVVLPDLAAGCSMADMATAAQVDDCWDDLIDAGVAEHTIPVSY
                     MNSSAAIKAFTGRHGGTICTSSNARTALEWALEQTGGVEGTGKVLFLPDQHLGRNTAV
                     RELGLSLDDCVVYDPHRPGGGLTKEAMQAARVILWRGHCSVHGRFTVDAVQAARREIP
                     GVRVIVHPECQYEVCEAADVIGSTEQIIKAVAAAPAGTSWVVGTELNLVKRLSTMFPE
                     QSIAFLEKNVCYCSTMNRIDLPHLVWTLESLAEG"
     gene            1189..2775
                     /locus_tag="PLK64_14270"
     CDS             1189..2775
                     /locus_tag="PLK64_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HPV80957.1"
                     /translation="MLDLLRLLSQQPILLLAILLGLGSLLGSFRYKGVHLGPAAVLFG
                     AIAISSLGTAYGLKLELPEVVGTLGLVLFTYTVGLLSGPNFFASLRRGWVAMVAVVGV
                     FVAVGFVGVLGGRLLGLSSPVIAGTFAGALTNPPALAAATERAGDTTGPTIGYSIAYL
                     YGVAAMLVAAMLVLRRSSDDRARDPLTNLTIRVDLDAPICAHEIVRDSGGTVVFSRIQ
                     HGEENPIEVIDEHEILQAGDLVTVVGPAGAVSGVAERLGHRSSHALQSQRRDLDFRRI
                     TVSQAALAGRTIAELGLDERFGAVATRVRRGDLDMVASEDFVLQMGDRVRVTAPSGRL
                     KEVSAYLGDSERGLSDINPIGFALGLAVGVALGLVHIPAPGGGFTLGAAAGTLLVGLV
                     FGRLVRVGPLVVSMSTSSATSLSTFGMITFLAYAGSRAGERFVASVTSDLGWKVLVLG
                     ALLTTVAAVAIAFVGRSVLKTNSRQLAGMLAGAQTQPAVLAFANDRTGFDLRVGLGYA
                     MVYPAAMIAKILLAQVIAGL"
     gene            2810..4048
                     /locus_tag="PLK64_14275"
     CDS             2810..4048
                     /locus_tag="PLK64_14275"
                     /inference="COORDINATES: protein motif:HMM:NF014634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HPV80958.1"
                     /translation="MSPVRVLIAPEGYAGLLAPAEAAAAIARGWSLGAPHDSIEVLPV
                     SDGGPGFIDTVARALGGEVQVVMVDDPWGRPTPATMLVSTDGATVYLEAAQAGGGQVQ
                     QPGFGAQATQATHSPDGTAGDDPAAASSGGVGDLLEAALRLRPRRIVLGLTGSLVLDA
                     GLGMLGRLIPDQPSTAAGRAALSGGAAHLVLLRADNLGALDTAIGRFGATQLVALGDL
                     HQPLLGLAGVAATHGPALGADPEHAQLIEAALGHAVDLVRRARPERRDLLSGTSVRLD
                     RQPGAGAGAGLGFGLAVLGAALRDGATEGPQVVGLAQAVARADLVVTGSTILGWEALR
                     SSASAAVAHAAQEGGIPAIALCGRVEAGRRETMAAGYSGTYAVASSLATWPAFAADPA
                     RALAVRAESVARTWSPPTPA"
BASE COUNT          529 a         1460 c         1460 g          670 t
ORIGIN      
        1 ccctcggcga gcgactcaag cgtccacacc aggtggggca ggtcgatgcg gttcatcgtc
       61 gagcagtagc agacgttctt ctcgaggaag gcgatcgact gctcggggaa catggtcgac
      121 aggcgcttga ccaggttgag ttcggtgccg accacccagg aggtcccggc cggagcagcc
      181 gccacggcct tgatgatctg ctcggtcgag ccgatgacgt cggccgcctc gcagacctcg
      241 tactggcact cggggtggac gatcactcgg accccgggga tctcgcgccg ggccgcttgc
      301 accgcgtcga ccgtgaaccg gccgtggacc gagcagtgcc cccgccacag gatgacccgg
      361 gcggcctgca tggcctcctt ggtgagcccg ccgccggggc ggtgcgggtc gtagacgacg
      421 cagtcgtcca gcgacagacc caactcccgg accgccgtgt tgcgacccag gtgctggtcg
      481 ggcaggaaca acaccttgcc ggtgccctcg acgccgccgg tctgctccaa ggcccactcc
      541 agcgcagtgc gggcgttgga cgacgtgcag atcgtgccgc cgtgccgacc cgtgaacgcc
      601 ttgatcgcgg ccgaggagtt catatacgac acggggatgg tgtgctccgc gaccccggcg
      661 tcgatgaggt cgtcccagca gtcgtcgacc tgggccgcgg tggccatgtc ggccatcgag
      721 cagccggccg ccagatcggg gagcacgacc gtctgggcat cggtggtgag gatgtcggcc
      781 gattcggcca tgaagtgcac gccgcagaag acgatccact ctgcctccgg gcgggcggcg
      841 gcctgctgcg ccagcttgaa cgagtcgccg gtcacgtccg cgaactcgat gacctcgtcg
      901 cgctgatagt ggtgcccgag cacgaacacc ttgtccccga gcgccgcctt ggccgcgcgg
      961 gcgcgtcgga ccagatcggg gtcggacggg gcgggcaggt cgccggggca ttcgacgccg
     1021 cgttcgctgt gcaggtcgcg gccctgcccc aggacgagca ggccgagggg agtgtgcttc
     1081 ggggtcaccg tttcgatgct cacaaaggca agggtatgcc gtccgcagca gtccgcggcc
     1141 cgctgcacgg ttggcgctgg gacccttcga cgtgcaaggg tggagtacgt gcttgacctg
     1201 cttcgcttgc tgtcccaaca accgatcctg ctgcttgcca tccttctggg gctcggctcg
     1261 ctgttgggat ccttccgata taagggagtt cacctcgggc cggcggcggt cctcttcggc
     1321 gcgatcgcga tctccagcct tgggacggca tacgggctca agctcgagct tcccgaagtg
     1381 gtggggacgc tcgggctggt cctgtttacc tacacggtag gcctgctgtc gggacccaac
     1441 ttcttcgcct cgctgcgccg cggctgggtg gcgatggtcg cggtcgtcgg cgtgtttgtg
     1501 gcagtcggtt tcgtgggcgt cctcggtggc cggctgctcg gcctgagcag cccggtcatc
     1561 gctgggacgt tcgccggtgc gctgaccaat ccacccgcac ttgctgcagc gaccgaacgc
     1621 gcgggcgaca cgacgggtcc aaccatcggc tactccatcg cctaccttta cggcgttgcc
     1681 gccatgctcg ttgccgcgat gctcgtcctg cgccgaagca gtgacgatcg cgctcgtgat
     1741 ccgctgacca acctcaccat ccgggtcgac ctcgacgcgc cgatctgcgc ccacgagatc
     1801 gtccgggaca gtggcggcac cgtcgtcttc tcgcgcatcc agcacggcga ggagaacccc
     1861 atcgaggtga tcgacgagca cgagattctg caggcaggag acctcgtcac ggtcgtcggc
     1921 ccggcgggtg ccgtgagcgg cgtcgccgag cgcctgggcc atcggtcctc gcacgcgctg
     1981 cagtcccagc gtcgcgacct cgacttccgc cggatcaccg tgtcgcaggc agcgctggcg
     2041 ggccggacga tcgccgagct ggggctcgac gagcgcttcg gtgcggtcgc cacgcgggtg
     2101 cgtcgcggcg acctcgacat ggtggccagc gaagacttcg tcctgcagat gggcgaccgg
     2161 gtgcgggtga ccgctccgtc aggtcggctc aaggaggtca gcgcctacct cggcgacagc
     2221 gagcgcggcc tgtcggacat caaccccatt gggttcgccc tcggccttgc tgtcggagtt
     2281 gccctcgggt tggtccacat ccctgcgccc ggcggtggct tcaccctcgg tgctgcggct
     2341 gggaccctgc tcgtcgggtt ggtcttcggg cgcctggtgc gggtgggacc cctggtcgtc
     2401 tcgatgtcca cgtcctcggc gacctcgttg tcgaccttcg gcatgatcac cttcctcgcg
     2461 tatgccggtt cgcgagccgg tgagcggttc gtcgcgtcgg tcacctcgga cctggggtgg
     2521 aaggtgctcg tccttggggc gctcctcacc accgtggccg ccgtggcgat cgcgttcgtc
     2581 ggccggtcgg tgctcaagac caactcgcgg cagttggcgg ggatgctcgc cggcgcgcag
     2641 acccagccgg cggtgctcgc cttcgccaac gaccggaccg gcttcgacct ccgggtcggg
     2701 ctgggctacg cgatggtcta tcccgcggcg atgatcgcca agatcctcct ggcacaagtg
     2761 atcgccgggt tgtagcccac ctcccgcaga cggcccgact cgtggtgtga tgtcgcccgt
     2821 gcgtgtgctc atcgccccgg aggggtatgc cggcctgctc gcccctgccg aggcggctgc
     2881 ggcgatcgcg cgcggctggt ccttgggggc accgcacgac agcatcgagg tgctgccggt
     2941 gtcggacggc gggccgggct tcatcgacac cgtggccagg gccttgggcg gcgaggtgca
     3001 ggtagtgatg gtcgatgacc cctggggtcg cccgaccccg gcgacgatgc tcgtcagcac
     3061 ggacggcgcc acggtctatc tcgaagccgc ccaagccggc ggagggcagg tgcagcagcc
     3121 cggtttcggc gcccaggcca cccaggccac ccacagcccc gatggcacgg ccggcgatga
     3181 ccccgccgcc gccagctcgg gcggcgtggg tgacctgctg gaggcggctc tgcgcctgcg
     3241 cccacgacgg atcgtcctgg gcctcaccgg atcgctggtg ctcgacgccg gtctcgggat
     3301 gctgggccgg ctcatccccg accagccgtc cactgcggcg ggccgggctg cgctgagcgg
     3361 tggtgctgcc cacctggtgc tcctgcgcgc agacaacctc ggagccctcg acacggcgat
     3421 cgggcgattc ggcgccaccc agctcgtcgc cctcggcgac ctgcaccagc cgctgctcgg
     3481 cctcgccggt gtcgcggcga cccacggccc tgcgctcggc gccgaccccg agcacgccca
     3541 actcatcgag gcggccctgg gccacgctgt cgatctggtc cgccgagccc gtcccgagcg
     3601 gcgggacctg ctgtccggca cctccgtgcg gctggatcgt cagccgggcg ccggagccgg
     3661 agcagggctc ggtttcggtc tggccgtgct cggagccgcg ctgcgtgacg gcgcgaccga
     3721 gggaccgcag gtcgtcggcc tggcccaagc cgtcgctcgg gccgacctcg tcgtcaccgg
     3781 ctcgacgatc ctgggctggg aggccttgcg gtctagtgcg agcgcggcgg tcgcacacgc
     3841 ggcccaggag ggcggcatac cggccatcgc cctctgcggg cgcgtggagg cgggccgtcg
     3901 cgagaccatg gccgcgggct attccgggac gtatgccgtc gcgagctccc tggccacctg
     3961 gcccgccttc gccgccgacc cggcccgcgc cctggccgtc cgagccgaga gtgttgcccg
     4021 gacctggtcg ccgccgaccc ccgcctgacg cggcacctga ctcgctgcca cacgcgggcg
     4081 ccggtgcgtc acagacgcgc acaaccggga cgtacggac
//
LOCUS       DAOMZY010000310         4030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_21148_length_4030_cov_5.193962, whole genome shotgun sequence.
ACCESSION   DAOMZY010000310 DAOMZY010000000
VERSION     DAOMZY010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21148_length_4030_cov_5.193962"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PLK64_14280"
     CDS             complement(<1..549)
                     /locus_tag="PLK64_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPV80959.1"
                     /translation="MPLRDFRIHRYVNAFCPHCHAERPDQPLADVRRLSGWLAARDGK
                     VWLERGCPEHGFVRTLYDESAEILTYLEQWTATTKEHVSDVMGNFAPVPAAYLDGMPH
                     MQTQHTCILLADITDTCNLRCPTCFAESAPARATTAPLAEVLASVDDRLAKERGRLDV
                     VMLSGGEPTLHPDLFAVLDEAKS"
     gene            complement(576..1388)
                     /locus_tag="PLK64_14285"
     CDS             complement(576..1388)
                     /locus_tag="PLK64_14285"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPV80960.1"
                     /translation="MFPTLGDLFGIVLAVPTHDTFVGLGVMVAVAVFIVETRRRGVAD
                     YRLLYVVTGALVGGAIFMRLGPLLQHLDLGANPSLAQQWVYGNRSVLGGLFGAWLGVH
                     VSKRLAGYSPRTGDLFAPAVAIGMAIGRFGCLFTELPGSPNPLGFGPVLDDATAARLS
                     GVAGTPLHPSYAYEIAFHAIAFVVLWRVLRLRMQAPGETLVVYLAAYGVFRFCVEFTR
                     GEEAVWAGLDRSQLFLLATVPILLARVVWQWRRGAYVLAPRHTPAQLAGSLP"
     gene            complement(1398..1889)
                     /locus_tag="PLK64_14290"
     CDS             complement(1398..1889)
                     /locus_tag="PLK64_14290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV80961.1"
                     /translation="MQIRDAKPDDLPAIAPIFREIVADGETYAYPEDLTDAQIAAAWM
                     EPPPARCIVAYAADGTILGTAKAGPNRPGRGDHIATASFMVSPTAQGQGVGRALAEEV
                     LRWATDQGYAAMQFNAVVETNMGAVALWQQLGFRILTTVPGTFRSRHHGPVGLHVMFR
                     ELG"
     gene            1921..3150
                     /locus_tag="PLK64_14295"
     CDS             1921..3150
                     /locus_tag="PLK64_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HPV80962.1"
                     /translation="MADSTSGADDLVLEATEQRVLGSLLEKQVTVPSTYPMSLNAIRS
                     ACNQATSREPVTDFDEATVQESLRGLRLRELVRVVHGDRVLKYHQLLGQRLELADDER
                     ALITVLLLRGPQSPGELKTRTDRLHAFADRTEVEECLARLASREVPLVVELPKAPGHH
                     DARWVHLLGPVELPAAVAAAVAAPVVDREVVLAGGAAQRDVNVTAAYDAVATAYALEF
                     GTELEGKPFDRWLLARVAQLAGIDPVADVGCGPGHVTRFLAEAGASVSGFDVSPGMVE
                     EARLGSPDLGFEVADFRGLMRPRLAPAWGAIVAWYAVIHLAESELASAVAGLARVLRP
                     GGWLALATHVGDAVHHVEEFLGEPVQLDFVLHDAGAVREAVAAAGLEVVEWYVRGPIA
                     GAEAQTDRIYILARKPD"
     gene            complement(3280..3978)
                     /gene="msrA"
                     /locus_tag="PLK64_14300"
     CDS             complement(3280..3978)
                     /gene="msrA"
                     /locus_tag="PLK64_14300"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HPV80963.1"
                     /translation="MGFLFGRRTSFVTREEALPGRHTRPYAVSPTHVVLGTPLEGPWP
                     AGSEVVYLAMGCFWGVERIFWRLPGVIVTAAGYMGGYTPNPTYEETCTGATAHAETVQ
                     VVYDPAVTSISALLKVFWENHDPTQGNRQGNDVGTAYRSAIYWTTPEQEAAALATQDA
                     FQKVLRDNGFGAITTEIRSAADAGPFYLAEDYHQGYLHKNPAGYCNHGPNGMTCPVGI
                     ANLPAQQEIRPPSA"
BASE COUNT          616 a         1322 c         1475 g          617 t
ORIGIN      
        1 ggacttcgcc tcgtcgagga cggcgaacag atcggggtgc agcgtgggct cgccaccgga
       61 gagcatgacg acgtccaggc gccctcgctc cttggcgagg cgatcatcga cggaggcgag
      121 cacctccgcg aggggtgctg tcgttgcccg tgccggcgcc gactcagcga aacacgtggg
      181 gcagcgcagg ttgcacgtat cggtgatgtc cgccagcaag atgcaggtgt gctgggtctg
      241 catgtgcggc ataccgtcga ggtatgccgc ggggaccggc gcgaagttgc ccatgacgtc
      301 ggagacgtgt tccttggtcg tcgcggtcca ctgctcgagg taggtgagga tctcggccga
      361 ctcgtcatag agcgtccgga cgaatccgtg ctcggggcaa ccgcgttcga gccagacctt
      421 gccgtcgcgc gcggccagcc accccgacag gcgacgcacg tcggccaggg gctggtcggg
      481 tcgctcggcg tggcagtggg ggcagaacgc gttgacgtaa cggtgaattc ggaagtctcg
      541 caacggcata ccggggccga cgctcgggcg ggcactcacg gcaggctccc tgcgagctgc
      601 gccggagtat gccgtggcgc gaggacatag gcgccgcgcc gccactgcca gacgacccgg
      661 gccaggagga tgggaacggt cgcgaggagg aacagttggg agcggtccag gccggcccag
      721 acggcctcct cgccgcgggt gaactcgacg cagaaccgga agacgccgta tgccgcgagg
      781 tagacgacga gggtctcgcc gggcgcctgc atccgaagcc gaaggactcg ccagaggacg
      841 acgaaggcga tggcgtggaa ggcgatctca taggcgtagg acgggtgcag aggcgttccg
      901 gcgaccccgc tgagccgggc tgctgtcgcg tcgtccagga cgggtccgaa gcccagcggg
      961 ttgggtgaac cgggcagctc ggtgaacagg cagccgaagc gtccgatcgc catcccgatg
     1021 gccacggcgg gggcgaacag atcgccggtg cggggggagt agccggccag ccgcttgctc
     1081 acatgaacgc cgagccaggc accgaacagg ccgccaagga ccgagcggtt gccatagacc
     1141 cattgctgcg ccagcgacgg gttcgccccg agatcgaggt gctgcagcag aggacccagg
     1201 cgcatgaaga tcgccccgcc cacgagggcc ccggtgacga catagagcag gcggtagtcg
     1261 gcgacgcccc ggcggcgcgt ctccacgatg aagacggcga cggccaccat caccccgagg
     1321 ccgacgaacg tgtcgtgggt cggcaccgct agaacgatgc cgaacagatc ccccagggtt
     1381 ggaaacactg ccccacgcta tccgagctct cggaacatga cgtgcagccc gaccggcccg
     1441 tgatggcggc tgcggaaggt ccccgggacg gtggtgagga ttcggaagcc gagttgctgc
     1501 cacagcgcga cggcgcccat gttcgtctcg acgaccgcgt tgaactgcat cgcggcatag
     1561 ccctggtcgg tggcccaccg caggacctcc tcggcgagcg cgcggccgac gccttgcccc
     1621 tgggcggtcg gcgagaccat gaacgaggcg gtcgcgatgt ggtcgccgcg gccgggccgg
     1681 ttgggcccgg ctttggcggt cccgaggatg gtgccgtccg cggcatacgc gacgatgcag
     1741 cgcgccggcg gaggttccat ccacgcggcg gcgatctgcg cgtcggtgag gtcttccggg
     1801 taggcgtagg tctcgccgtc ggcgacgatc tcgcgaaaga tcggggcaat ggcggggagg
     1861 tcatcgggct tcgcgtcgcg gatctgcacg ttggcagtat gccgcgctag attcccgagc
     1921 atggctgact cgacgtccgg cgctgatgac ctcgtgctcg aagcgaccga gcaacgcgtc
     1981 ctcgggagcc tgctggagaa gcaggtcacc gtcccctcga cctaccccat gtcgctcaac
     2041 gcgattcggt cagcctgcaa ccaggccacc agtcgcgagc cggtcacgga ctttgacgag
     2101 gcgaccgtcc aggagagtct gcggggtttg cggctgcgtg agctcgtgcg ggtcgtgcat
     2161 ggcgaccggg tcctgaagta tcaccaactc ctcgggcagc ggctcgagct cgccgacgac
     2221 gagcgggccc tcatcaccgt gctgttgctg cgcggcccgc agtctccggg cgagctcaag
     2281 acccgcaccg atcggttgca cgcctttgcc gaccgcaccg aggtcgagga gtgcctggcg
     2341 aggctcgcct cgcgcgaggt tccgctggtg gtcgagttgc ccaaggcgcc tgggcatcac
     2401 gacgcccgct gggtgcatct gctcggcccg gtcgagttgc ctgcggccgt tgctgctgcg
     2461 gtcgccgcgc cggtcgtgga ccgggaggtc gtcctcgccg gcggcgcggc ccagcgcgac
     2521 gtgaacgtga cggcggccta cgacgcggtc gcgacggcat acgccctgga gttcgggacc
     2581 gagctggaag gcaagccgtt cgaccgctgg ctgttggcgc gagtggcgca gttggcgggg
     2641 atcgacccgg tggccgacgt ggggtgcggc ccggggcacg tcacccggtt cctcgccgag
     2701 gcgggtgctt cggtgagcgg attcgacgtg tccccgggga tggtcgagga ggcgcgactg
     2761 ggatcgccgg acctcggttt cgaggtcgca gacttccgtg ggctcatgcg ccctcggctc
     2821 gcccccgcgt ggggcgccat cgtcgcctgg tatgccgtca tccatcttgc cgagtccgaa
     2881 ctggcttctg cggtggctgg attggcgcgg gtgctgcgcc ccggaggctg gctcgcgctg
     2941 gccactcatg tcggcgacgc ggtccaccat gtggaggagt tcctcgggga gccggtgcag
     3001 ttggacttcg tcctgcacga tgccggggcg gtgcgcgaag cggtcgcggc ggccgggctc
     3061 gaggtggtcg agtggtatgt ccgtggtccg atcgccgggg ccgaagcgca gaccgaccgg
     3121 atctacatcc tcgcccgcaa acccgactga ccgctccgag ctcggccccg ggaatgggtt
     3181 gggtgcgggc ggggatgagg ccgctcatga agccgtcgta ggtggtgtga tcacgacacc
     3241 tacgacggct tcataggcgg cttcccgccc gcgccgaact caggcgctcg gcggacggat
     3301 ctcctgctgc gcgggcaagt tcgcgatgcc gaccgggcag gtcatgccgt tggggccgtg
     3361 gttgcagtag cccgcggggt tcttgtgcag atagccctgg tggtagtcct cggcgagata
     3421 gaacggcccc gcgtctgccg ccgagcgaat ctcggtcgtg atcgccccaa agccgttgtc
     3481 ccgcagcacc ttctggaaag catcctgggt ggccagcgcc gctgcctcct gctcgggcgt
     3541 cgtccagtag atcgccgacc ggtaggccgt gccgacgtcg ttgccctgcc ggttcccctg
     3601 cgtcgggtcg tggttctccc agaacacctt gagcagcgcc gagatcgacg tgaccgcagg
     3661 gtcatagacc acctggaccg tctcggcgtg cgccgtcgcc ccggtgcagg tctcctcata
     3721 cgtcgggttc ggcgtgtaac cacccatgta gcccgccgcc gtaacgatca cgcccggcag
     3781 ccgccagaag atccgctcga cgccccagaa gcaccccatc gcgagataga cgacctcgga
     3841 gcccgccggc cacgggcctt cgagcggcgt tccgaggacg acgtgggtgg gggagacggc
     3901 atatgggcgg gtatgccgcc ctggcagcgc ctcctcgcgc gtgacgaagg atgtccgacg
     3961 gccgaaaagg aaacccatcg ggtcagtatc gccgataccc tccccggatg gtcccggccg
     4021 cgcctccacc
//
LOCUS       DAOMZY010000311         4029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_21153_length_4029_cov_7.060393, whole genome shotgun sequence.
ACCESSION   DAOMZY010000311 DAOMZY010000000
VERSION     DAOMZY010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21153_length_4029_cov_7.060393"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1201)
                     /locus_tag="PLK64_14305"
     CDS             complement(<1..1201)
                     /locus_tag="PLK64_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPV80964.1"
                     /translation="MAASRNASQNVTTARTASGRLSFAQIHEPLEVPDLLALQTESFD
                     WLLGNERWQARVAAAKASGRTDVPDRSGLEEIFEEISPIEDFSGSMSLSFRDHRFEET
                     RKTIESCKERDQTYDAPLFVTAEFMNTNTGEIKSQTVFMGDFPLMTERGTFIINGTER
                     VVVSQLVRSPGAYFERTADKTSDKDIYTAKLIPSRGAWLEFEIDKRDMVGVRVDRKRK
                     QSVTVLLKALGWTNDQILENFGDYESMRLTLEKDHTQGQDDALLDIYRKLRPGEPPTK
                     EAAQALLDNLYFNPKRYDLAKVGRYKLGKKLGVEAPLSASTLSIDDIVATIRYIVALH
                     AGETTLPGRRHGEAVELLVETDDIDHFGNRRLRSVGELIQNQVRTGLSRMERVVRERM
                     TTQDVEAI"
     gene            1709..2008
                     /locus_tag="PLK64_14310"
     CDS             1709..2008
                     /locus_tag="PLK64_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HPV80965.1"
                     /translation="MARIAELSADMDALFEASRDSNADDEHDPEGQTIAYERSQLAAI
                     TAQTRDRLDEVEAALERVSDGTYGRCEVCGEPIPLARLEARPAARTCVRHAETRR"
     gene            complement(2166..2555)
                     /gene="rplL"
                     /locus_tag="PLK64_14315"
     CDS             complement(2166..2555)
                     /gene="rplL"
                     /locus_tag="PLK64_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006548361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPV80966.1"
                     /translation="MAKLSTEELLEAFKEMTLIELSEFVKQFEETFGVTAAAPVAVGV
                     AAVGGGSADAPAEEEKDEFDVVLVAAGDKKIQVIKEVRTLTSLGLKEAKDLVDGAPKA
                     VLEKVDKVAAEKAKELLEGAGATVELK"
     gene            complement(2613..3266)
                     /gene="rplJ"
                     /locus_tag="PLK64_14320"
     CDS             complement(2613..3266)
                     /gene="rplJ"
                     /locus_tag="PLK64_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPV80967.1"
                     /translation="MATAAKSAAIAELTDAFRSSGAAVLTEYRGLTVKQLTQLRSSMR
                     DHATYAVVKNTLTEIAAKEAGVTAFDGQLAGPSAIAFITGDPVEAAKTLRDFAKANPL
                     LVIKSGMLDGKPLTADDIRKLADLESREVLLAKLAGALQASLSKAVYLFAAPLSQAAR
                     TVDALRAKVAESAPVADAPAAAAAPQDEVPQDEAPADAAPADADAVAAVQDSSTDDA"
     gene            complement(3627..>4029)
                     /locus_tag="PLK64_14325"
     CDS             complement(3627..>4029)
                     /locus_tag="PLK64_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048290.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HPV80968.1"
                     /translation="HLIDRANLLTLSALEMTVLVGGLRVLGANYQGSRLGVLTDRTDT
                     LSNDFFTNLLDLGIHWTPTDEAQDTFEGRDRATGEVRWTGSRVDLVFGSNSELRALAE
                     VYASDDAQASFVHDFVAAWDKVMNLGRYGRD"
BASE COUNT          648 a         1318 c         1387 g          676 t
ORIGIN      
        1 tgatcgcctc gacgtcctgg gtggtcatcc gctcgcgaac cacacgctcc atccgggaca
       61 ggcccgtgcg gacctggttc tggatgagct cgccgacgct gcgcagacga cggttgccga
      121 agtggtcgat gtcgtcggtc tcgacgagca gctcgaccgc ctcgccgtgg cgacgaccgg
      181 gcagggtcgt ctcgccggcg tgcagggcca cgatgtagcg gatggtcgcg acgatgtcgt
      241 cgatcgacag cgtggaggcc gacagcggcg cctcgacccc gagcttcttg cccagcttgt
      301 accggccaac cttggccagg tcatagcgct tggggttgaa gtagaggttg tccagcaggg
      361 cctgcgcggc ctccttggtc ggcggctcgc ccgggcgcag cttgcggtag atgtccagca
      421 gcgcgtcgtc ttggccctgg gtgtggtcct tctccagggt cagacgcatc gactcgtagt
      481 ccccgaagtt ctcgaggatc tggtcgttgg tccagccgag cgccttgagc agcacggtga
      541 ccgactgctt gcgcttgcgg tcgacacgga cgccgaccat gtcgcgcttg tcgatctcga
      601 actccagcca ggcgccgcgg ctcgggatga gcttcgcggt gtagatgtcc ttgtcggacg
      661 tcttgtcggc agtgcgctcg aagtaggcgc cgggcgaccg gaccaactgc gagacgacga
      721 cccgctcggt gccgttgatg atgaacgtcc cgcgctcggt catgagcggg aagtcaccca
      781 tgaagaccgt ctggctcttg atctcgccag tgttggtgtt catgaactcg gcggtcacga
      841 acaagggcgc gtcgtaggtc tggtcgcgct ccttgcagga ctcgatggtc ttgcgggtct
      901 cctcgaaccg gtggtcgcgg aaagacagcg acatcgatcc cgagaagtcc tcgatggggg
      961 agatttcctc gaagatctct tcgaggccgg agcggtcggg aacgtcagtg cgcccggatg
     1021 ccttggccgc cgcgacgcga gcctgccagc gctcgttgcc gagcagccag tcgaagctct
     1081 ccgtttgcag cgccaacaga tctggaacct cgagtggctc gtggatctgg gcgaagctca
     1141 gtcgacctga ggccgtccga gcggttgtga cgttctggga cgcgttgcgc gaggcagcca
     1201 acgggggtcc ttccacaggc gtgctgttcg tggttgacgc gtcgccctca cccagactcc
     1261 atgccgacgg accgcaacgt gacgaatgca cacagcagcg ctcagggtcg gtagggggtc
     1321 agggtatggg cagcgcaaag tatgagcata gccggaagaa ggcacgactg tccagctgag
     1381 tcaagtgccc caggcgtgcg attcgctcag cgaccgcccg gtgacgggac ttcggtagtc
     1441 atgaggatga ctggtatcga cccagacgtc aaggtaagcc gcctcacgtc ctccccgcgt
     1501 gtcgccggag cctccaacgc ggctccccca cacctgcgct gacggcctgg ttgcgccgca
     1561 cctgtctacc cctcgaatat ccttgcggca caatccatct agttcccaga cacaaggatg
     1621 gctgatgccg cgtcgcgacc catctcccga tccacggccc gagattgctg gcgtagtcgc
     1681 cctcctgcgc gaggacctcg agtccctcat ggccaggatt gcggaactct ccgcggacat
     1741 ggatgccctg ttcgaggcct cgcgcgactc caacgccgat gacgaacacg acccggaagg
     1801 ccagacgatc gcttacgagc gctcccaact cgcggcgatc acggcccaga cccgcgatcg
     1861 gctcgacgag gtggaagcag ccctggagcg ggtcagcgac gggacctacg gcaggtgcga
     1921 ggtgtgtggt gaacccatcc cgctcgcccg attggaggcg cgcccggcgg cccggacgtg
     1981 cgtccggcac gccgagacgc gccgctagca gccgcaccga ccacgaccac gcgcatgagc
     2041 acgcgaaagg gggccggcac tggcctcagc gcgggcacgc tactgcgcca acgacaacgc
     2101 ggggcgggtc cgaagtggac ccgccccgcg tcagcagtgc gtgagacggc gtatgccgcg
     2161 tcagctcact tgagctcgac ggtggcgccg gcgccctcga ggagctcctt ggccttctcg
     2221 gcggcaacct tgtcgacctt ctccaggacg gccttgggcg cgccgtcgac gaggtccttg
     2281 gcctccttca ggccgaggga ggtgagggtg cgaacctcct tgatgacctg gatcttcttg
     2341 tcgccggcgg caacgaggac gacgtcgaac tcgtccttct cctcctcggc cggggcgtca
     2401 gccgagccgc cgccaaccgc ggcaacgccg acggcaaccg gagcagcagc ggtgaccccg
     2461 aaggtctcct caaactgctt gacgaactcg gagagctcga tgagggtcat ttccttgaaa
     2521 gcctcgagga gctcctcggt gctgagcttc gccatggtgg cgatcctttc tcttggtgcc
     2581 agtgagtggc gggtgatgga agacgtcgcc actcaggcgt cgtcggtgga gctgtcctgc
     2641 acagcggcga ccgcgtcggc gtcagccggc gcagcgtccg cgggagcctc gtcctgcgga
     2701 acctcgtcct gcggggccgc ggccgcggcg ggcgcgtcag cgaccggagc ggactcggca
     2761 accttggcgc gcaaggcgtc aacggtgcgc gcggcctgcg aaagcggagc cgcgaacagg
     2821 tagacggcct tggacagcga ggcctgcagg gcgcctgcca gcttggccag gagcacctca
     2881 cgggactcaa ggtcggccag cttgcggatg tcgtcggcgg tgagcggctt gccgtcaagc
     2941 atgccgctct tgatgacgag cagggggttg gccttggcga agtcccgcaa cgtcttggcc
     3001 gcttcgaccg ggtcaccggt gatgaaggcg atagccgacg ggcccgcgag ttgcccgtcg
     3061 aaggccgtga ctccggcctc cttcgcagcg atctcggtga gggtgttctt caccacggcg
     3121 taggtggcgt ggtcgcgcat cgaggagcgc agctgggtca gctgcttcac ggtcagaccg
     3181 cggtactcgg tgagcacggc cgcgcccgaa ctacggaacg cgtccgtcag ctcggcgatg
     3241 gctgctgact tggcagcggt ggccatcggg cctcctttac ctagcggtgc gacgccggtt
     3301 cgggctgcga agtcccccac gagaaaagcc ccggcgcgag gcgcacgggg cttcgcggca
     3361 taccaagagg tatgccgggt gcttgcgtgg cctgcgctgg tcgcccgcct cgtggcggga
     3421 ccttcggtca tcccggcctc cagtttcctg gcggacggca cgacaaccgg cggtcattgg
     3481 tcgttgctca gggtagtcgg gcgcaggccg gcagccaaat ccgctaccga acggcgtccg
     3541 gtcgaccggc ctccagacgg acggacaggc tcacggacgg gcccggtggc gtaaccacca
     3601 ccgggcccgt ccgtcaggcg acaggctcag tcgcggccgt accgcccgag gttcatcacc
     3661 ttgtcccacg cagcgacgaa gtcgtggacg aagctcgcct gggcgtcgtc gctcgcatag
     3721 acctcggcaa gtgctcgcaa ctcggagttg gaaccgaaga ccaggtcgac ccgactgccg
     3781 gtccatcgga cctcgccggt ggcccggtcc cggccctcga acgtgtcctg ggcctcgtcg
     3841 gtcggcgtcc agtggatgcc gaggtcgagc aggttagtga agaagtcgtt cgagagggtg
     3901 tcggtgcgat cggtgaggac accgagacgg gacccctggt agttggcacc cagcacccgc
     3961 agcccgccga cgagcaccgt catctcgaga gcactgagcg tgagcaggtt agcccggtcg
     4021 atgaggtgg
//
LOCUS       DAOMZY010000312         4002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_21343_length_4002_cov_7.204459, whole genome shotgun sequence.
ACCESSION   DAOMZY010000312 DAOMZY010000000
VERSION     DAOMZY010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21343_length_4002_cov_7.204459"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PLK64_14330"
     CDS             complement(<1..393)
                     /locus_tag="PLK64_14330"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HPV80969.1"
                     /translation="MRVFRATLGVIGELLVTAGVVVLLFVVWQVGYSSLIEGRAQAGV
                     VAELEALGPDGTSLPELPEGPLVGGVAPTLDDGSIMAIIRIPRFGTDWARPVYEGITP
                     GTLAKGPGHYPRSSMPGQIGNMAIAGHRT"
     gene            complement(390..1130)
                     /locus_tag="PLK64_14335"
     CDS             complement(390..1130)
                     /locus_tag="PLK64_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HPV80970.1"
                     /translation="MPPASPRSRPGYWRLLAPAVFALAGLLFATSFQAARGTDLRSDR
                     GLPGLIDGATRDVAAKSAAITTLQTELDALTAAAVPSTTGLAPITDVINLLAPVVGTR
                     AVQGPAVTVALTDSKRNSANLPASVRPDDLVVHQQDVQAVVNALWRGGAEAMMIQDQR
                     IIATSAVRCVGNTLILQGRVYSPPFVISAIGRIDALNEALDQDPQIAIYKEWVDAVGL
                     GYRVTTEAKATFPAYSGPVSQLVAKVAR"
     gene            1240..1542
                     /locus_tag="PLK64_14340"
     CDS             1240..1542
                     /locus_tag="PLK64_14340"
                     /inference="COORDINATES: protein motif:HMM:NF018487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HPV80971.1"
                     /translation="MSESQDPTDKKPVVVPPAAKVAQARQAAEAEKRRQAKALDAPNP
                     SWWAPVFVTLLLAGLLWIVVFYISQGLWPVAAFNYWNLVVGFALLIAGFAMTMKWK"
     gene            complement(1646..2401)
                     /locus_tag="PLK64_14345"
     CDS             complement(1646..2401)
                     /locus_tag="PLK64_14345"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPV80972.1"
                     /translation="MDCVRESARTVRPTRTAFGGRTIDGNPLATKAIIAICAAVYVLQ
                     FLRPVTTEDLAFVPVIGWHEPWRALTGAFLHSPSTVLHLGLNMLMLWQIGPYLESLLG
                     RARFLALYLVSAVGGSAGVLLLAPAPRSILFTSAEAAQYAAWATPVVGASGAVFGLFG
                     ALLVLNRHLGRSSTPLYAVLAVNIVFGFIYPGIAWQAHLGGLVTGTAVAGVFAVLAHR
                     DRRSWQWPAMALVFALVIAAMVIDYLGVPDIYR"
     gene            complement(2606..3127)
                     /locus_tag="PLK64_14350"
     CDS             complement(2606..3127)
                     /locus_tag="PLK64_14350"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPV80973.1"
                     /translation="MKATLHTNHGDIVVTLFPNEAPKTVANFVGLATGEKDYRDDAKR
                     TNPTKFYDGLVFHRIISGFMIQGGCPLGQGYGGPGFTFDDEPHPDKTFDRPYLLAMAN
                     AGKRMGKGTNGSQFFITVSTPGYLNFKHTIFGEVADAPSRAIVDAIAAVRTDRNDRPL
                     EPVVIESVTVDQD"
     gene            3365..>4002
                     /locus_tag="PLK64_14355"
     CDS             3365..>4002
                     /locus_tag="PLK64_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80974.1"
                     /translation="MSRKRASDKTEDLSVFGRLAAARGERGDGTVFGALAQLRESVAP
                     AAQRGRTRAMQAAAPLADRAREVATPLAERAKDATAPYAGRARDAADAAREAAAPYAG
                     RAREAAAPYAGRARDAAEAAREAAAPYAEKAKEAAGAAREAAAPYAERAKEAAEAGRE
                     WARPRVEHGVEVAAPRLQAAVDEIAPKVDQARDSLVEDLLPKVSAAIAAVAAA"
BASE COUNT          647 a         1332 c         1461 g          562 t
ORIGIN      
        1 ggtgcggtgg ccagcgatcg ccatgttgcc gatctggccg ggcatcgatg accgtgggta
       61 gtggcccggt cccttcgcca gggtgccggg ggtgatccct tcgtagaccg gcctggccca
      121 gtcggtgccg aaccgcggga ttcggatgat cgccatgatt gagccgtcat caagcgtggg
      181 cgcaacgccc ccgaccaggg ggccttccgg aagctcaggg aggctcgtgc cgtctggccc
      241 taacgcctcc aactccgcga cgacccccgc ttgcgcccgg ccttcgatga ggctgctgta
      301 gcccacctgc cagacgacga agagcagcac gacgacgccc gcggtgacca gcagttcgcc
      361 gatcacgccg agcgttgcgc ggaagaccct cacctcgcca ccttcgccac cagttgggac
      421 acgggacctg agtatgccgg gaaggtcgcc ttcgcctcgg tcgtcacccg atagccgaga
      481 ccgaccgcgt cgacccactc cttgtagatg gcgatctgcg ggtcctggtc gagcgcctcg
      541 ttgagggcgt cgatgcgccc gatcgccgag atgacgaacg gcggcgaata gacccggccc
      601 tgcaggatca acgtgttgcc cacgcaccga acggccgagg tcgcgatgat ccgttggtcc
      661 tggatcatca tcgcctcggc cccgccccgc cacagcgcgt tgacgaccgc ttggacgtcc
      721 tgctggtgga cgacgaggtc atcgggtcgc accgatgccg gcaggttggc cgagttgcgc
      781 ttggagtcgg tgagcgccac cgtaaccgcc ggcccctgca cggctcgggt gccgacgacc
      841 ggtgcgagga ggttgatgac gtcggtgatc ggggccaggc cggtcgtcga gggcaccgcg
      901 gcggcggtga gggcgtcgag ctcggtctgc agggtcgtga tggccgccga cttggcggcc
      961 acgtcgcggg tggccccgtc aatgagacca ggcaagccac ggtcgctgcg cagatcggtg
     1021 ccacgggcgg cctggaagct ggtggcgaag agcaggccgg ccagcgcgaa caccgctggg
     1081 gccagcaggc gccagtagcc tggacgggat cgaggggagg cgggcggcat accgacacct
     1141 cctgaggtac tagtcgagcc cgcgcgcaag cgagcggcac gcgagtgcgt gctccatcac
     1201 ctacgctaag gcacaccagc aagaacgcaa ggagacctcg tgtccgagtc gcaggacccc
     1261 accgacaaga agccggtcgt tgtcccgccg gcagccaagg ttgcccaggc gcgccaggcg
     1321 gccgaggccg agaagcgccg ccaggctaag gccctcgatg cgcccaaccc gtcgtggtgg
     1381 gcgccggtct tcgtcacgct cctgcttgcg gggctgctgt ggatcgtcgt cttctacatc
     1441 tcgcagggcc tctggccggt tgcggcgttc aactactgga acctcgtcgt cggcttcgcg
     1501 ctgctcatcg ccgggttcgc catgacgatg aagtggaagt gacctcggcc cactcatcgg
     1561 gtgtccggcg agttacatcc gtgtagttct ccccagctgt ggacaagcct gtggataact
     1621 tcgccggccg ctccgccggg gccgctcagc ggtagatgtc gggcacgccg aggtagtcga
     1681 taaccatcgc ggcgatgacc agagcaaaca ccagggccat cgccggccac tgccacgagc
     1741 gccgatcccg atgggccagc acggcgaaca ccccggcaac ggccgtgccc gtgacgaggc
     1801 cacccaagtg ggcctgccag gcgatcccgg gatagatgaa cccgaagacg atgttgacgg
     1861 ccagcacggc atacagcggc gtcgaggatc gaccgagatg ccggttgagc acgaggagcg
     1921 ccccgaacag cccgaagacc gccccggagg cgccgacgac gggagtggcc caggcggcat
     1981 actgcgcggc ctcggccgag gtgaacagga tgctgcgcgg cgcgggggcc agcagcagca
     2041 cgccggccga cccgccgacg gccgagacga ggtagagcgc caggaagcgg gcccggccga
     2101 gcaggctctc gagatagggc ccgatctgcc agagcatgag catgttgagc ccgaggtgca
     2161 acaccgtgga cgggctgtgc aggaacgcgc cggtgagggc gcgccacggc tcgtgccacc
     2221 cgatgacggg gacgaaggcg aggtcctcgg tcgtcaccgg gcgcaggaac tgcaagacat
     2281 agaccgcggc gcagatggcg atgatcgcct tggtggccag ggggttgccg tcgatggtgc
     2341 gcccaccgaa ggcggtgcgc gtcggacgga ccgtgcgggc cgactcgcgc acacaatcca
     2401 cgcattgcac gcccacgggc gccggccgtt ggcactccgg gcacaccggc cgacgacacc
     2461 gttgacaact cacccacgac tctcggtcgg ggtggcgggg gcagaccggg cggggggtcg
     2521 ccccaccgac gccctcaggc agggtgggat agctcatgta tgccgcccgc tcacctcggt
     2581 cgcgcgcctg cccactgcgg gtggctcagt cctggtcgac ggtgaccgac tcgatgacga
     2641 ccggctcaag cgggcggtcg ttgcggtcgg tgcgcacggc ggcgatcgcg tcgacgatcg
     2701 cacggctcgg ggcgtcggcc acctcgccga agatcgtgtg cttgaagttg aggtaacccg
     2761 gcgtcgagac ggtgatgaag aactgcgagc cgttggtgcc cttgcccatc cgcttcccgg
     2821 cgttggccat ggccagcagg taggggcggt cgaaggtctt gtcggggtgc ggctcgtcgt
     2881 cgaaggtgaa gcccggcccg ccatagccct gacccaacgg gcagccgccc tggatcatga
     2941 agccggaaat gatccggtgg aagaccagac cgtcgtagaa cttggtcggg ttcgtccgct
     3001 tggcgtcgtc gcggtagtcc ttctcgcccg tcgcgagccc cacgaagttg gccaccgtct
     3061 tgggcgcctc gttcgggaag agggtgacga cgatgtcgcc gtggttggtg tggagggttg
     3121 ccttcatggc gaacatcctc gcacggcata cccagaggta tgccgggtgt cccagacggc
     3181 gctccagcgt gcgttcgggg gaggcgggtg tgtgtctgga cccgggacag ggcaggatgt
     3241 gaccactgac cccggcagcg gcgggcgttg acgacagtgt cggcgacaac gacgatgagg
     3301 cgacgacgcc gcgaggggtc caggtgcttg gatggacgac caccacgaac caccaggagg
     3361 atcagtgtcc cggaagcgtg cgagtgacaa gaccgaggac ctgtcggtgt ttgggcggct
     3421 ggccgcggcc cgcggcgagc gcggtgatgg caccgtcttc ggtgcgctgg cccagctgag
     3481 ggagtcggtg gcaccggcgg cccagcgcgg gcgcacccga gcgatgcagg ccgctgcccc
     3541 cctcgccgac cgggctcgcg aggtggctac tcccttggcc gagcgggcca aggacgccac
     3601 ggccccgtat gccggtcggg cgcgcgacgc cgccgatgcc gcgcgtgagg cagccgcacc
     3661 gtatgccggt cgcgcgcgtg aggcagccgc accgtacgcc ggtcgggccc gcgacgccgc
     3721 ggaggccgcc cgcgaagcgg ccgcacctta tgccgagaag gctaaggaag ccgcaggggc
     3781 tgctcgcgaa gcggcggcgc cgtatgccga gcgggccaag gaagccgcgg aggccgggcg
     3841 ggagtgggct cggccgcgcg tcgagcatgg agtcgaggtc gccgccccac ggctgcaggc
     3901 cgcggtcgat gaaatcgccc cgaaggtgga tcaggcccgc gacagcctcg tcgaggacct
     3961 tctgcccaag gtgtccgccg ccatcgccgc cgttgccgcc gc
//
LOCUS       DAOMZY010000313         3959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_21672_length_3959_cov_4.703381, whole genome shotgun sequence.
ACCESSION   DAOMZY010000313 DAOMZY010000000
VERSION     DAOMZY010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21672_length_3959_cov_4.703381"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PLK64_14360"
     CDS             <1..337
                     /locus_tag="PLK64_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HPV80975.1"
                     /translation="SSIEWDIHRIQTRGMMTGGLYNVSLRGTGYVAITTKGDPVALDV
                     AGAPTFGDAQAVVMWTSGVSMDIRVDTGGLSSMIRGGTGETFQMAFGGHGYVLIQPDE
                     GVVSGGHQA"
     gene            complement(365..778)
                     /gene="nusB"
                     /locus_tag="PLK64_14365"
     CDS             complement(365..778)
                     /gene="nusB"
                     /locus_tag="PLK64_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPV80976.1"
                     /translation="MGARTKSRKRALDILFEADQRGINAQQLLIERLARPATPAPLAD
                     YTITLVEGVVRCWSEINEMLATYSQGWSVERMPAVDRAALRLATWEIVYSDEVPDAVA
                     IDEAVELVKSLSTDESPAFVNGLLARIAEVKHTFG"
     gene            complement(784..1347)
                     /gene="efp"
                     /locus_tag="PLK64_14370"
     CDS             complement(784..1347)
                     /gene="efp"
                     /locus_tag="PLK64_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPV80977.1"
                     /translation="MATTNDLKNGLVLNIDGQLWAVIEFQHVKPGKGPAFVRTKLKNV
                     LSGKVVDRTFNAGVKVETANVDKRTMQYLYKDGSDFVFMDTDTYDQLHVSEAVVAGGA
                     DYLLENQNAIVATHDGVPLYVELPASVVLEITFTEPGLQGDRSTGGTKPATLETGAQI
                     QVPLFLESGTRVKVDTRDGSYLGRVND"
     gene            1779..2438
                     /locus_tag="PLK64_14375"
     CDS             1779..2438
                     /locus_tag="PLK64_14375"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPV80978.1"
                     /translation="MFRITIPTRHAAVVYHAGRLVDVLPAGRTRRRWGHHYVLVDLRE
                     RQQVIPPQDIPTADGLGVRVSAVARWAITDPVAFLEVSQTPADTLHLATQLALREAVG
                     ALAAADLVVALRRLDVTAPVDAATRQVGIATLGVAVRDVVLPHDLRIATAEVVTARLR
                     GAAALEAARAETAALRALANGARLLDEHPALAQLRLVQSAPPGTRLVLQLGGTPEPAG
                     D"
     gene            complement(2535..>3959)
                     /locus_tag="PLK64_14380"
     CDS             complement(2535..>3959)
                     /locus_tag="PLK64_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80979.1"
                     /translation="DNLVLALLGRRASERDRALVDVAVGAHVNLTGTVTLAAVDAARA
                     AGNTPNTVIAAALAIAGPGRAEASRHAIHGFIDLFTGSGLSVDDTETDLAAYPAPPEL
                     RALAIGADPDPIAEAMLAGLAERGVTSMPLRWLATQGGHPQVPGVVAAIAGTLVWKSL
                     AHKEISRITAEELPWWLELFGTMIGAAAPTAAHTAEGFCGIPMAMVLGQASVTELAAA
                     ALLGERPSADILDGFAMLTGLLLTNGPGAITAQGAKGAVSADGPQTPERIQLNKAMIG
                     FLTHAGFAHGGNGFEGIAFLLERFAGTGLVDPGDPAHGVDLDALAREYAVAFAELKTT
                     RKELGADRATIPCINHPVFKGKDVNVDPREEFVRTVMAQRGDYNVFHDYYRRLVVALR
                     DAGATTNVFCVNVDALIATGLLKMLWSRYRDGSFTEGQLENAAFTVFLFGRMIGCAAE
                     IDDHQNRGRNMDTRSPAKVLSFVS"
BASE COUNT          572 a         1377 c         1384 g          626 t
ORIGIN      
        1 gtcctcgatc gagtgggaca tccaccggat ccaaacccgc ggcatgatga ccggcggtct
       61 ctataacgtg tcgctgcgtg gcaccggcta cgtcgccatc accactaagg gcgaccccgt
      121 ggccctcgac gtcgccgggg cgccgacctt cggcgatgcc caggccgtcg tcatgtggac
      181 ctccggtgtc tcgatggaca tccgggtcga caccggcggg ctcagctcga tgatccgcgg
      241 cggcaccggc gagaccttcc agatggcctt cggcggccac gggtacgtcc tcatccagcc
      301 tgacgagggt gtcgtcagcg ggggtcacca ggcgtagcgc ccgggaaacg gacgacggca
      361 ccggtcagcc gaaggtgtgt ttgacctcgg cgatccgggc cagcaggccg ttgacgaacg
      421 ccggcgattc gtcagtcgac aggctcttga ccaactcgac cgcctcgtcg atggcgaccg
      481 cgtcagggac ctcgtcgctg taaacgatct cccaggtcgc taggcgcaag gctgcgcggt
      541 cgacggccgg catccgctcg acgctccacc cttggctata ggtcgcgagc atctcgttga
      601 tctctgacca acaccgcacg actccctcga caagggtgat cgtgtagtcc gccaacggcg
      661 ccggagtcgc cggacgcgcc agccgctcga tgagcagttg ctgcgcgttg atcccgcgct
      721 ggtcggcctc gaaaaggatg tccagcgcgc gtttgcgtga cttcgtgcgg gcgcccacca
      781 gcgtcagtcg ttgacgcggc cgaggtagga gccgtcgcgg gtgtcgacct tgacccgggt
      841 gccgctctcg aggaagagcg ggacctggat ctgggcaccg gtctcaagcg tggccggctt
      901 ggtgccgccg gtcgagcggt caccctgcaa gcccggttcg gtgaaggtga tctccaggac
      961 caccgacgcg ggcaactcga catagagggg caccccgtcg tgggtggcga cgatggcgtt
     1021 ctggttctcc agcaggtagt cggcgccacc ggcgacgacc gcctcagaca cgtgcagctg
     1081 gtcgtaggtg tcggtgtcca tgaagacgaa gtccgacccg tccttgtaga ggtactgcat
     1141 cgtgcgcttg tcgacgttgg ccgtctcgac cttgacgccc gcgttgaacg tgcggtcgac
     1201 gaccttgccg gacaggacgt tcttgagctt ggtccgcacg aacgcggggc ccttgccggg
     1261 cttgacgtgc tggaactcga tgacggccca caactgcccg tcgatgttca gcacgaggcc
     1321 gttcttgagg tcgttcgttg ttgccacggg tggtcgcttc cttgctcgcg ggtatgccgg
     1381 gcgcgccagc gcccgtcgaa gatctggtgg gattcggctc cagtctaccg gaaaaccgtt
     1441 tgccgcctcg cgctcgcgcc tggcaccctc gacacgtcgg ccgagggagc agccggggcg
     1501 gcataccggt tcccggtcct gcgcacgacc tggccgacac aggtcttggg aacctgctag
     1561 tcggctcacg ccaccagggg tgcgactccc ctttctggtg acgccgggcg gatcacccga
     1621 cccaacggcc ctgcggccca cccccgcgct gccccggcag tgcggtgggt gtcgccccgc
     1681 ccgacggtcg ccgcccggcg ccactccctg ccccttgccc ctgccccttg cccctacccc
     1741 acgcccctac ccaacgatgc accaaggagg tgcccgtcat gttccgaatc acgatcccga
     1801 cccgtcacgc cgcggtcgtc taccacgccg gccggttggt cgacgtcctg cccgccgggc
     1861 gcacccggcg tcggtggggg caccactacg tgctggtcga cctgcgcgaa cgccagcagg
     1921 tcatcccgcc ccaggacatc ccgaccgccg acggtctggg cgttcgcgtc tcggcggtcg
     1981 cgcgttgggc gatcaccgac ccggtcgcct tcctcgaggt ctcccagacg ccggccgaca
     2041 cccttcacct ggcgacccag ttggcgctgc gggaggcggt gggcgccctc gccgcggctg
     2101 acctggtggt cgcgctgcgg cggctggacg tcacggcgcc ggtcgacgcg gcaactcgtc
     2161 aggtcggcat cgcgacgctt ggcgttgcgg tgcgcgacgt cgtcctgccg cacgacctgc
     2221 ggatcgcgac cgccgaggtg gtcacggccc gcctgcgggg cgcagccgcg ctcgaggcgg
     2281 cccgcgccga gaccgcggcc ctgcgggcgt tggccaacgg ggcccggctg ctcgacgagc
     2341 acccggcgct ggctcagttg cgcctcgtcc agtccgcgcc gcccggcact cgccttgtgc
     2401 tgcaactggg cggaacgccg gagccggcgg gcgactgacc acgcccgacg ctcgccggtt
     2461 gtgggcgatt ggcggcctgc cgggtatgcc gtgcgcgcgc agtgtgcaca cggcataccc
     2521 ggccctggcc ggggtcagga gacgaaggag agcaccttcg cgggcgagcg ggtgtccatg
     2581 ttgcgcccgc ggttctggtg gtcgtcgatc tcggcagcac acccgatcat ccggccaaag
     2641 aggaacaccg tgaaggcggc gttctccaac tgaccttcgg tgaatgaccc gtcccggtac
     2701 cggctccaga gcatcttgag caggcccgtc gcgatgagcg catcgacgtt gacgcagaac
     2761 acgttggtgg tcgcgccggc gtcgcgcagc gcgacgacga gtcgacggta gtagtcgtgg
     2821 aagacgttgt agtcaccacg ctgcgccatg accgtccgga cgaactcctc gcgcgggtcg
     2881 acgttgacgt ccttgccctt gaagaccggg tggttgatgc aggggatggt cgcgcgatcg
     2941 gcacccaact ccttgcgggt cgtcttcaac tcggcgaagg cgacggcata ctccctcgcg
     3001 agggcgtcga ggtcgacgcc gtgagcgggg tcgcccgggt cgacgaggcc ggtgcccgcg
     3061 aaccgctcca gcaggaacgc gatcccctcg aagccgttgc ccccgtgggc aaaaccggca
     3121 tgcgtgagga aaccgatcat cgccttgttg agctggatcc gctcgggggt ctgtgggccg
     3181 tcggccgaga ccgccccctt ggcgccctgg gcggtgatgg cacccgggcc gttggtgagc
     3241 aacagtccgg tgagcatcgc gaagccgtcc aagatgtcgg cggagggacg ctcgcccagc
     3301 agggctgcgg cagcgagctc cgtcaccgac gcctgaccga gaaccatcgc catgggtatg
     3361 ccgcagaaac cctcggcggt gtgcgccgcg gtcggtgcgg cggcgccgat catcgtgccg
     3421 aacagctcca accaccaggg cagttcctct gccgtgatcc gagagatctc cttgtgcgcc
     3481 aaggacttcc agacgagagt cccagcgatg gcggccacga caccggggac ctggggatgc
     3541 ccgccctggg tcgccaacca gcgcagcggc atcgaggtca cgccgcgttc ggccaggccc
     3601 gcgagcatcg cctcggcgat cgggtcggga tcggcgccga tcgccagggc gcgcaactcg
     3661 ggcggcgccg ggtatgccgc gaggtcggtc tccgtgtcgt cgaccgacag cccgctgccg
     3721 gtgaagaggt cgatgaagcc atggatggca tgtcgcgagg cctcggcgcg gcccggcccg
     3781 gcgatggcga gcgcggcggc gatgaccgtg ttgggggtgt taccggcagc ccgggccgcg
     3841 tcgacggctg cgagggtgac ggtgccggtg aggttgacgt gggcaccaac cgccacgtcg
     3901 acgagcgccc ggtcgcgctc gctcgcgcga cggccgagca gggccaggac gaggttgtc
//
LOCUS       DAOMZY010000314         3955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_21700_length_3955_cov_6.125128, whole genome shotgun sequence.
ACCESSION   DAOMZY010000314 DAOMZY010000000
VERSION     DAOMZY010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21700_length_3955_cov_6.125128"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /gene="galK"
                     /locus_tag="PLK64_14385"
     CDS             complement(<1..1051)
                     /gene="galK"
                     /locus_tag="PLK64_14385"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HPV80980.1"
                     /translation="MAMAPIDRALRLFQDAFGGEPAGVWSAPGRVNLIGEHTDYNGGL
                     ALPIALPQRTYAAVRVRPDGVLRVVSDGMPGGPVSVPLAEVGPGSPGGWAAYVAGVFW
                     ALRDAGYAVPGADVALASSVPVGAGLSSSAAVECSVGAALSDLADLGLLADLDGRRRL
                     ATVCQQAENDIAGAPTGGMDQLASMLGTAGHALLLDCRDGSTQAVPFDLAAHGLALLV
                     VDTRAPHALVDGQYAARRHTCQEAATALGVSSLREVGLPYLADTLARLPDDVTRRRVR
                     HVVTEIDRVGRFADAVEAADWRLAGTLMVASHESLRDDYEVSCQELDLVVESAMAAGA
                     WGARMTGGGFGGCAIA"
     gene            1107..1712
                     /locus_tag="PLK64_14390"
     CDS             1107..1712
                     /locus_tag="PLK64_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HPV80981.1"
                     /translation="MSADLRSRDAVTMGPGADWSADAQDHRQTIGVAIRVPAPFGPLL
                     QAKRAEFKDPLADLIPAHVTLLGPTVIHQVARPALVEHLEQVSREVGPFPMALRGTGT
                     FRPVSDVVFVQVARGISGCERLEQRIRSGEYAAELTFPYHPHVTVAHDVAEADLDRAF
                     DDLADFRADFDVEEIWLFEQDAAGQWRAVRSFRLTGEEQTR"
     gene            1709..2899
                     /locus_tag="PLK64_14395"
     CDS             1709..2899
                     /locus_tag="PLK64_14395"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HPV80982.1"
                     /translation="MTAAEASRAEPEAGRAEAGRAELSAIRRLWARAQRTEAWRAWQR
                     YGVAQGGLLAAGIGYFSFFSLFPAVALAAVVFGFILQGRPDLLAAIGDALNAALPGFV
                     KTDANPSGLVALTAPETATLSWTGAVAIVTLVAGGLGWIGSLGEGLRAMFGVRKHPGN
                     IVVAKVRDLVVLGLLGVALLVSATLTSAVGAAGAWSAQHLGLGEHPWLVGIAGVLVSL
                     LVDMAIMVVLLRVLTGLKLPWPVVRAGALIGGGAMTLLKLVGAQLVTRATSNPVFGSL
                     VVVVGLLFWLNLMAKVVLLSAAWAAGDLDDSGMPSTRAAAAASTQAAGRDMAGPDDAD
                     GALDGASGGIDAGDSRARAVNGIPNLGVRSKDRVTLAAGAVLGASAAIAGGAVRRLAR
                     PRQR"
     gene            complement(2890..>3955)
                     /locus_tag="PLK64_14400"
     CDS             complement(2890..>3955)
                     /locus_tag="PLK64_14400"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80983.1"
                     /translation="NPHAWLLPASTLKTLTALVVIPALDPDRVVIGEEEDTAADGSRV
                     GITVGGTYTVGDLLNGLILSSGNDAAYALARTYGGRPALLAAMNARAGSLGAWDTVAA
                     DPSGLDAPGQHSSAYDLALIARAVMSLPGYASRASLPVAMFPGGLVPASPTTKAGGKV
                     VAPTASLTSGPPFQIDNHNQLLGVYPGVIGVKNGYTSKARNTYIGAVRLNGRTLLITT
                     MGSPEPQAPSAVALLDWGFAYAAQLHPVGTLVADGSAPQPPELGGATASASPSTTSAE
                     PWTGTGASSPDAAARAGATAAAATPIERVVSAALSGWWGGLPEAARWGLLGALALTLA
                     AAAWLGVRRWRRRARGVYQR"
BASE COUNT          552 a         1390 c         1438 g          575 t
ORIGIN      
        1 gcgcgatcgc gcacccgccg aacccgccgc ctgtcatccg tgcgccccat gccccagcag
       61 ccatcgccga ctcgacgacg agatcgagct cctgacaact cacctcgtag tcgtcgcgga
      121 gggactcgtg cgaggccacc atgagcgtcc ccgcgagccg ccagtcagcg gcttccacgg
      181 cgtcggcgaa ccggccgacg cggtcgatct cggtgacgac gtgacggact cgacggcggg
      241 tcacgtcgtc ggggagccgt gcgagcgtgt cggcgaggta aggaagcccg acctcacgca
      301 ggctgctcac cccgagcgcc gtggcggctt cctggcaggt atgccgtcgc gcggcatact
      361 gcccgtccac gagggcgtgc ggggcgcgcg tgtcgacgac gagcaacgcc aacccgtgag
      421 cggccaggtc gaaaggaacc gcctgggtcg acccgtcacg gcagtccaac agcagggcgt
      481 gtccggccgt gccgagcatc gacgccagtt ggtccatgcc gccggtgggt gccccggcga
      541 tgtcgttctc ggcctgctgg cagaccgtgg ccagccgacg acgtccgtcg aggtcggcga
      601 gcaggccgag gtcggcgagg tcggacagcg cggcgccgac cgaacactcc acggccgccg
      661 acgaggacag gccggctccg acgggcaccg aactggctag agccacatcg gcgccgggca
      721 cggcataccc cgcgtcacga agggcccaga agactccggc gacgtaggcc gcccacccgc
      781 cgggggagcc ggggcccacc tcggcgagcg gcacgctgac ggggccaccc ggcataccgt
      841 cgctgacgac ccgcaggacg ccgtcggggc gcacgcggac ggccgcgtag gtgcgttgcg
      901 ggagcgcgat gggcagcgcc aggccgccgt tgtagtcggt gtgctcaccg atgaggttga
      961 cccgaccggg tgccgaccag accccggcgg gctcgccgcc gaacgcgtcc tggaagagtc
     1021 gcagcgctcg atcgatcggt gccatcgcca tgtcgggaag cgtagcgatg acgaacgggc
     1081 ccaacgaagt cgatacgctg acacccatgt ccgccgacct gaggtctcga gacgccgtca
     1141 cgatggggcc tggtgcggac tggtccgcgg acgcgcagga tcaccgccag accatcggcg
     1201 tggccatccg cgtccccgct ccgttcggtc cgttgttgca ggccaaacgg gccgagttca
     1261 aggacccgct cgccgatctc atcccggccc acgtcacact cctgggcccg accgtcatcc
     1321 accaggtggc gcgtccggct ctggtcgagc acctggagca ggtgagccgc gaggtgggcc
     1381 cgttcccgat ggcgttacgc gggacgggca cgttccggcc ggtctcggat gtcgtgttcg
     1441 tccaggtggc ccgaggcatc tccggatgcg agcggcttga gcagcgcatc cgcagcgggg
     1501 agtatgccgc ggagctcacc ttcccctacc acccgcacgt cacggtcgcc cacgacgttg
     1561 ccgaagccga cctcgaccgg gccttcgacg acctcgccga tttccgtgcc gacttcgacg
     1621 tcgaggagat ctggctcttc gagcaggatg ctgcgggaca gtggcgggcg gtgcgatcgt
     1681 tccggctcac cggtgaggag cagacccgat gaccgcggct gaagccagtc gtgcggagcc
     1741 tgaagccggt cgcgcggaag caggtcgcgc cgagctcagc gcgatccgcc ggctatgggc
     1801 ccgggcgcag cgcaccgaag cctggcgggc ctggcagcgc tatggggtgg cccagggggg
     1861 cctgttggcc gccgggatcg gctacttctc gttcttctcg ctctttcctg ccgtggcact
     1921 ggcagcggtg gtcttcggct tcatcctgca gggccggccc gacctgctcg cggccatcgg
     1981 cgatgccctc aacgccgcgc tcccgggctt cgtcaagacc gacgccaacc cgagcgggct
     2041 ggtggcgttg accgctcccg aaaccgccac cctcagctgg acgggagcgg tcgcgatcgt
     2101 caccctcgtg gccggtggtc tcggctggat cggctcgcta ggcgaagggt tgcgggcgat
     2161 gttcggggtg cgcaaacatc ccggcaacat cgtcgtcgcc aaagtgcgcg acctggtcgt
     2221 cctgggactg ttgggggtgg cccttctcgt gtcggccacc ctcacctcgg ccgtcggtgc
     2281 ggccggagcg tggagcgccc agcacctcgg gctgggcgag catccatggc tggtcggcat
     2341 cgccggggtc ctcgtctcgc tgctcgtcga catggcgatc atggtcgtcc tgctgcgcgt
     2401 gctcaccggt ctgaaactgc cctggccggt cgtccgggcc ggcgccctga tcggcggcgg
     2461 cgccatgacg ctgctcaaac tcgttggggc acaactcgtg acgcgggcca cgagcaaccc
     2521 ggtcttcggg tcgctcgtcg ttgtcgttgg gctgttgttc tggctcaacc tgatggccaa
     2581 ggtcgtgctc ctctcggcag cgtgggcggc cggtgacctc gacgactcgg gtatgccgtc
     2641 cacccgcgct gccgcagccg cctcgaccca ggcggccggc cgagacatgg cggggcctga
     2701 tgacgccgac ggggcgctcg atggggccag cggcgggatc gatgccggcg actcgcgggc
     2761 ccgagcggtg aacggcatac ccaacctggg agtccggagc aaggaccggg tcacgctcgc
     2821 tgcgggcgcc gtgctgggcg ccagcgccgc gatcgcgggc ggcgcagttc gccgcctggc
     2881 gcgccctcgt cagcgctgat agacccctcg ggcgcgtcgg cgccaacgcc ggacacccag
     2941 ccaggctgca gcggcgagcg tcaacgccag cgccccgagc aggccccaac gcgccgcctc
     3001 ggggagccct ccccaccacc cggacagcgc ggcgctaacg acgcgttcga tcggggttgc
     3061 ggctgcggcg gtggcgccgg ccctcgcggc ggcatccgga gagctcgccc ccgtccccgt
     3121 ccaaggctcg gccgacgtag tcgacggcga cgctgacgcc gtcgctccgc ccaactccgg
     3181 aggctgaggt gcggatccgt cggcgacgag ggtgccgacg gggtgcagtt gcgcggcata
     3241 cgcgaagccc cagtcgagca acgcgacagc cgacggagcc tgcggctcgg ggctgcccat
     3301 cgtcgtgatg agcaaggtgc gcccattgag gcggacggct ccgatgtagg tgttccgcgc
     3361 cttgctcgtg tagccgttct tgacgccgat cacgccggga tagaccccga ggagttggtt
     3421 gtggttgtcg atctggaagg gcggcccgct ggtcaggctg gcggtggggg ccacgacctt
     3481 gcccccggcc ttcgtggtcg gcgatgccgg gaccagccct ccggggaaca tcgcaacggg
     3541 cagcgacgcc cgggaggcgt agcccggcag gctcatgacc gctcgggcga tgagcgccag
     3601 gtcgtaggcg ctggaatgct gtcccggggc gtccagtccc gagggatcgg cggccacggt
     3661 gtcccacgca ccgaggcttc cggctcgggc gttcatggcc gcaagcaggg cggggcgccc
     3721 gccgtaggtg cgagccaggg cgtatgccgc gtcgttgccg ctgctgagga tgagcccgtt
     3781 gagcaggtcg cccacggtgt aggtgccacc caccgtgatg cccactcggg agccgtcggc
     3841 cgcggtgtcc tcctcctcgc cgatgacgac ccggtcgggg tccagcgccg ggatgacgac
     3901 cagtgcggtg agggtcttga gcgtgctcgc cggaagcagc caggcgtgcg ggttc
//
LOCUS       DAOMZY010000315         3954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_21707_length_3954_cov_5.009490, whole genome shotgun sequence.
ACCESSION   DAOMZY010000315 DAOMZY010000000
VERSION     DAOMZY010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21707_length_3954_cov_5.009490"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="PLK64_14405"
     CDS             complement(<1..1049)
                     /locus_tag="PLK64_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80984.1"
                     /translation="MSERACWDCGGNRYDFTTTPGGLLCMACRRRRHYHPATCPQCGD
                     LRPLAFHHDDRIVCASCAGVASVFACRDCGREDQPYGLQHCARCILRERLTSLLTDPA
                     TGAVHHRLQPVFDELVNSERPQTGIWWLRKKPGIGPALLGQMARGETPISHDTFRALP
                     ATRTREYVRMLLTATGVLPPVDYPIERVLAWLDGLLDPLPDPHADLVRRYAHWHVLRG
                     MRRAASRGHLTRSTTTAARNQIRAAVRFLTWLDTHNVTVADATQHHLEDYQQSPASDR
                     SADGSFIRWLRDSRINTRLRVATLPATQPAVTISDEQRWEHVHRLLHDPSLRSYTRLG
                     GLFTLLFAQPLHRIAA"
     gene            complement(1046..1375)
                     /locus_tag="PLK64_14410"
     CDS             complement(1046..1375)
                     /locus_tag="PLK64_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010157051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPV80985.1"
                     /translation="MTTRVSFYWNLRQVMAENGIFQTTDLVAPLAERGIHLSRQMIHR
                     VVTKTPQRINVELLAALCDILNCNAGDLLQLRREHVRDLPIAVNDNGPGIGDLKPVRA
                     TIRRPHE"
     gene            complement(1375..2475)
                     /locus_tag="PLK64_14415"
     CDS             complement(1375..2475)
                     /locus_tag="PLK64_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPV80986.1"
                     /translation="MTVPGAAHLVLAPGIAHLNEPVAVFEAMVSGWARQQRSRLLVDA
                     TVEPRIALIRRFTEVTDSYPWVWTAGDVEDFTLELMIGPDRLAPSTIRGYHHTLRLFC
                     DYLLDGRYGWVEQCEQRFGMIPSQVCHDFNTAAHLVEYEGRPERRPFTYDELQHLFDY
                     VDTRVERAQRSGRKGALAALRDAQMIKTAYAFGLRRRELCMLDVADLRPNPSMPAWGT
                     YGAIHVRYGKSSGGGVPRRRTVLSVPEFDWVIEGLRQWVDQARPLLQPGPRQELWLTE
                     RQQRVSVKTMDHRFAYLRKQAELPAELTLHCLRHSYVTHLVEFGYPERFVTEQVGHSY
                     ASTTAIYTSVSNDFKTKTLQAALSRVYGAKED"
     gene            2780..3097
                     /locus_tag="PLK64_14420"
     CDS             2780..3097
                     /locus_tag="PLK64_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80987.1"
                     /translation="MGRQQDRVAARQAIHEARIARRREQREREAAVEQLAVQVQVELR
                     AGARGLAEAEARAGALIEQMITVHGLTARDVATWCADGMTAREASRLRRVAVESTDED
                     PVA"
     gene            3154..3492
                     /locus_tag="PLK64_14425"
     CDS             3154..3492
                     /locus_tag="PLK64_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80988.1"
                     /translation="MMEPRTAGESVERVAGLLVTAQERAGLATWDLDQPRAFLRESAV
                     ELAAWQALDLLPLGTQVSPVAVAGAGVVAVLVEAEAELQRFAIGEYPRGTSALVARVG
                     DLLSEYRCPA"
     gene            3480..>3954
                     /locus_tag="PLK64_14430"
     CDS             3480..>3954
                     /locus_tag="PLK64_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80989.1"
                     /translation="MPGLNHSRADRLARALLSDPPTEPAAAILGLAYILQAAGERSSF
                     PVLQAVLGEHVGRAHDVGLALGEVTQPVRLLDASVQQPGCQGLEPLSTDGINLVGQES
                     GRVVGQQPPPLQQAPPVVNVRVESWAADGGQQPRRPCAGGINVYEIEHCRFSRNLS"
BASE COUNT          617 a         1165 c         1508 g          664 t
ORIGIN      
        1 gctgcgatcc ggtgcagggg ctgggcgaat agcagggtga acagcccgcc gaggcgcgtg
       61 tagcttcgca ggctggggtc gtggaggagc cggtggacgt gttcccagcg ttgctcgtcg
      121 ctgatggtga ccgcgggctg ggtcgcgggc agtgtggcga ctcgtaggcg ggtgttgatc
      181 cggctgtcgc ggagccagcg gatgaaggag ccgtcggcgg agcggtcgct ggccgggctc
      241 tgttggtagt cctcgaggtg gtgctgggtg gcgtcggcga cggtgacgtt gtgggtgtcg
      301 agccaggtga ggaatcggac ggcggcccgg atctggttgc gggcggcggt ggttgtggat
      361 ctggtgaggt gtccgcggct ggcggcccgc cgcatgccgc gcagcacatg ccagtgggcg
      421 tagcggcgga cgaggtcggc gtgggggtcg ggcagcgggt cgaggagccc gtcgagccag
      481 gcgagcaccc gttcgatggg gtagtcgacg ggtgggagga cgccggtcgc cgttagcagc
      541 atgcggacgt attcgcgggt gcgggtggcg ggcagggccc ggaaggtgtc atggctgatc
      601 ggggtttcgc cgcgggccat ctggccgagc agggcggggc cgatgcctgg cttcttgcgg
      661 agccaccaga tgccggtttg gggtcgttcg gagttgacca gttcgtcgaa cacgggctgt
      721 agtcggtggt ggacggcgcc ggtggcaggg tcggtgagca ggctggtgag gcgttcgcgc
      781 agaatgcagc gggcgcagtg ctggagaccg tagggctggt cttcgcggcc gcagtcgcgg
      841 caggcgaaga ccgatgcgac gccggcgcag gaggcgcaga cgatccggtc gtcgtggtgg
      901 aaggccaggg gcctcaggtc gccgcattgt gggcaggtcg ctgggtggta gtggcggcgt
      961 cgccggcagg ccatacacag cagtccgccg ggggtggtgg tgaagtcgta acggttcccg
     1021 ccgcagtccc agcaggcccg ttcgctcact cgtgggggcg acggatggtg gccctgacgg
     1081 gcttcaggtc gccgatcccc ggcccgttgt cgttgaccgc gatggggagg tcgcggacgt
     1141 gttcgcggcg cagttgcagc aggtcgccgg cgttgcagtt gaggatgtca cacagggcgg
     1201 cgagcagctc gacgttgatc cgttgcggtg tcttcgtcac gacccggtgg atcatttgac
     1261 gggacaggtg gatgccgcgc tctgccagcg gggcgaccag gtcggtggtc tggaagatgc
     1321 cgttctcggc catcacctga cgcaggttcc agtagaaact cacccgtgtg gtcatcagtc
     1381 ctccttggcc ccgtagacgc gggagagcgc ggcttggagc gttttggtct tgaaatcgtt
     1441 cgataccgag gtgtagatcg ccgtcgtcga cgcataggag tggccgacct gttcggtcac
     1501 gaaccgttcg gggtaaccga actcgaccag gtgggtcacg tacgagtgcc gcaggcagtg
     1561 cagagtcaac tcggccggaa gttcggcctg ttttcgcagg taggcgaacc gatggtccat
     1621 cgtcttgacc gagacacgct gttggcgttc ggtcaaccac agctcctgac gtggcccggg
     1681 ttgaagcagc ggccgtgcct ggtcgaccca ttgacgcagc ccctcgatca cccagtcgaa
     1741 ctcgggaacc gacaacacgg tccgtcgccg ggggacccca ccgccgctgg acttgccgta
     1801 acgcacatgg atcgcgccgt aagtgcccca cgccggcatg ctcgggttcg gccgaagatc
     1861 ggccacgtcc agcatgcaca actcgcgtcg tcggagcccg aacgcgtagg cggtcttgat
     1921 catttgagcg tcgcggagcg ccgccaacgc acccttccgg cccgagcgtt gggcacgctc
     1981 gacgcgggtg tcgacgtagt cgaacaggtg ctgcagctcg tcgtaggtga acggtcggcg
     2041 ttcgggtcgc ccttcgtact cgaccaggtg ggcggcggtg ttgaagtcgt ggcacacctg
     2101 ggaggggatc atcccgaaac gctgttcgca ctgctccacc cagccgtagc ggccatccag
     2161 cagatagtca cagaacaacc gcagggtgtg gtggtagccg cggatcgtcg agggcgccaa
     2221 ccggtcagga ccgatcatca actccagcgt gaagtcctcg acgtcgcctg cggtccacac
     2281 ccatgggtac gagtcggtca cctcggtgaa cctccggatc aacgcgatcc tcggctcgac
     2341 cgtcgcatcc accagcagcc gggaccgctg ctgccgagcc caacccgaca ccatcgcctc
     2401 gaacaccgcg acgggctcgt tcaagtgcgc gatcccgggg gccaggacca gatgagcagc
     2461 accgggaacg gtcaccaccc acccccagcc gtaaacttag aagtgacaat gacaatggaa
     2521 agttaccacg gtcatggcga cccgaacgcc acaaggcctg acgaaaccgg agacgggacc
     2581 acccacgacc cattgggtag acgcgcactc cgcaccgatc aactcagggt tgattcggga
     2641 cgctacgtgc ctcagtgcaa gaggtgtgtg gcgcttgaga ggccatgttc ccccgggggc
     2701 ttggttggtg ggtgcgctga gcacggcggg gtgccgggct ggcttgcgtc ttcgacattg
     2761 gtttggagag gagtcggaga tggggcgaca gcaggacagg gtggcggcac gacaggcgat
     2821 tcacgaggcg cggatcgcga ggcgtcgtga gcaacgggag cgtgaagcgg cggtggagca
     2881 gctggccgtc caggtacagg tggagctacg cgccggggcc cgggggcttg ccgaggccga
     2941 agcccgcgcc ggggcactga tcgagcagat gatcaccgtc cacgggctca ccgcccgtga
     3001 cgtggcgacc tggtgcgctg acgggatgac cgcccgggaa gcgagccgcc tgcgtcgtgt
     3061 tgcggtggag agcaccgacg aggatccggt ggcttgagtc gatacaggcc gcccgaacct
     3121 gggtgcggtc gaagcaacca ggaggcggcg acggtgatgg aaccacgtac agcgggcgag
     3181 agtgtggagc gggtcgcggg cttgttggtg acggcgcagg agcgcgccgg gttggcgacc
     3241 tgggatctgg atcagccgcg ggccttcctg cgggagtcgg ccgtcgagct ggcggcgtgg
     3301 caggcgcttg acctgctgcc gctcgggacg caggtgtctc cggtcgcggt ggccggtgcg
     3361 ggtgtggtgg cggtgctggt ggaggcggag gctgagctgc agcgcttcgc gatcggcgag
     3421 tatccgcggg gcacgagtgc tctggtcgcc cgggtggggg atctgctgtc ggagtaccga
     3481 tgcccggcct gaaccattcc cgcgcagacc gtctggcccg cgcgctgttg tccgatccgc
     3541 ctactgagcc ggctgcggcg atcctcggct tggcctacat cctgcaggcc gcgggggagc
     3601 gcagcagctt cccggtgctg caggcggttc tgggagaaca cgttggacga gcgcatgatg
     3661 tcggtctcgc cctgggtgag gtaacccagc cggtgcgcct cctcgatgcg tctgtccagc
     3721 agcccgggtg tcaaggcctg gagcccctca gcacggatgg catcaacctc gtggggcagg
     3781 agtcgggtcg cgtggtaggt cagcaaccac cgcccctcca gcaggccccg ccagtcgtga
     3841 atgtcagggt cgaatcctgg gccgctgacg gcggccagca gccgcgccgg ccatgtgctg
     3901 ggggcatcaa tgtctacgag atcgagcact gtaggttctc gcggaacttg tcac
//
LOCUS       DAOMZY010000316         3948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_21751_length_3948_cov_5.664269, whole genome shotgun sequence.
ACCESSION   DAOMZY010000316 DAOMZY010000000
VERSION     DAOMZY010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21751_length_3948_cov_5.664269"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="PLK64_14435"
     CDS             <1..1000
                     /locus_tag="PLK64_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HPV80990.1"
                     /translation="LAVARRTPGFTGADLANVLNEAALLTARTNKRAIDNQALDEAID
                     RVIAGPQKRTRIMSAKERKITAYHEGGHAIVAAAMNHTDPVTKVTILPRGRALGYTMV
                     MPIDDKYSTTRNEILDQLAYALGGRVAEEMVFHDPTTGAANDIEKATGMARKMVTEFG
                     MSERIGAIKLGQSTGEVFLGRDMGHQRDYSEQVAGVVDEEVRKLIENAHDEAWHVIND
                     NRDILDKLVLDLLEKETLGTKELAEIFAPLVKRPLRPTWLSSDARAISERPPVLTPAE
                     TAASSNGHVPSMPPPGSAPQDQGAAAAGYPPPVGEPTSPSGQAGSGSTGGPAPQGH"
     gene            1006..1578
                     /gene="folE"
                     /locus_tag="PLK64_14440"
     CDS             1006..1578
                     /gene="folE"
                     /locus_tag="PLK64_14440"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HPV80991.1"
                     /translation="MPVDHPRAQAAVRELLCAIGEDPDREGLRETPARVARALGEFFA
                     GLDQRPEDILSTTFDIDHDEMILVRDIEVYSTCEHHLVPFHGVAHVGYIPAKDGKVTG
                     LSKIARLVDVFAKRPQVQERLTTQVADALVDHLGVQGCIVVLECEHLCMSMRGVKKPG
                     SRTITSAVRGQLRNPATRAEAMALLLGGKR"
     gene            1665..2471
                     /gene="folP"
                     /locus_tag="PLK64_14445"
     CDS             1665..2471
                     /gene="folP"
                     /locus_tag="PLK64_14445"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPV80992.1"
                     /translation="MGVVNVTPDSFSDGGQWFEPDAAITHARGLLADGADLLDIGGES
                     TRPGASRPSVHEELSRVIPVVSALAAEGARVSIDTMRAEVAGAALAAGAALVNDVSGG
                     LADPQMLPLVAAAQVPFVVMHWRGHSVDMQDRAAYADVVAEVCRELASRRDAALAAGI
                     RADRLVLDPGLGFAKLAEHNWALLAGLPALRDLGQPILLGASRKAFLGKVGRPEHATP
                     RPAAERDVATAATTVLAGLAGVWAVRVHDVRSTRDALDVLEATQAHGGTP"
     gene            2468..3361
                     /gene="folK"
                     /locus_tag="PLK64_14450"
     CDS             2468..3361
                     /gene="folK"
                     /locus_tag="PLK64_14450"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPV80993.1"
                     /translation="MTDRIVVEGIRGLGYHGVFEHERRDGQQFLVDVAIELDLRRPGR
                     SDNLVDTVHYGEVAAQVLARIEGEPFDLIEALAEVIAADVLSLPGVEAVDVVVHKPQA
                     PVGVPVTDVRVEIRRERGTRVVIALGSNVGDRVATLTEAVEALAEVPRLALIAVSDLV
                     ESDPFGGPDQPAYLNGVVLANFAGSPATLLRALHAIEHRNGRTREIRWGARTLDLDLV
                     QFGVPGSDGERLSDTPALLLPHPRAHERNFVLRPWAHLDPEATLRVGPTVAHPVRLVA
                     DVLVELGLSGVRPGPVWSPAW"
     gene            3355..3849
                     /locus_tag="PLK64_14455"
     CDS             3355..3849
                     /locus_tag="PLK64_14455"
                     /inference="COORDINATES: protein motif:HMM:NF022819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3180 domain-containing protein"
                     /protein_id="HPV80994.1"
                     /translation="MVTSGGVRLRQLVVVTVVVGLLGLVVLRLLGGTVPSPGWPGVVL
                     LLFMAVGVYFAALPVKRLRERRVLSPGSALRAARSLVLAQAAALTGAGLLGWYAAQVV
                     VLLPNVDVDSQRGRMWLLVAHAVAAIVLVVSGLLAQRICRIDPPDAGDPGDRATNPGG
                     VAPA"
BASE COUNT          562 a         1432 c         1361 g          593 t
ORIGIN      
        1 gttggccgtc gcccgccgca cgcccggctt caccggcgcc gacctggcca acgtcctcaa
       61 cgaggccgcg ctgctcaccg cccgcaccaa caagcgggcg atcgacaacc aggccctgga
      121 cgaagccatc gaccgggtca tcgccggccc gcagaagcgc acccggatca tgtccgccaa
      181 ggaacgcaag atcacggcat accacgaggg tggtcacgcc atcgtggccg cggcgatgaa
      241 ccacaccgac cccgtgacca aggtgacgat cctgccgcgc ggccgggccc tcggttacac
      301 gatggtcatg ccgatcgacg acaagtactc caccacccgc aacgagatcc tcgaccaact
      361 cgcctacgcc ctcggcggcc gggtcgccga ggagatggtc ttccacgacc cgaccaccgg
      421 cgcggccaac gacatcgaga aggccaccgg gatggcccgc aagatggtca ccgagttcgg
      481 gatgagcgag cggatcggcg ctatcaagct cggtcagagc acgggcgagg tcttcctcgg
      541 ccgcgacatg ggccaccagc gcgactactc cgagcaggtt gccggcgtcg tcgacgaaga
      601 ggtgcgcaag ctcatcgaga acgctcacga cgaggcctgg cacgtcatca atgacaaccg
      661 cgacatcctc gacaagctcg tcctggacct cctcgagaag gagaccctgg gcaccaagga
      721 gctcgccgag atcttcgcgc ccctcgtcaa gcgcccgctg cgcccgacct ggctgtccag
      781 cgacgcccgc gccatcagcg agcgcccgcc ggtgctcacg cccgccgaga cggctgcgtc
      841 gagcaacggt cacgtgccct cgatgccgcc gcccggaagc gcaccgcagg accagggagc
      901 ggccgcggcc ggttacccgc cgcccgttgg cgagccgacc agtccctcgg ggcaggcggg
      961 ttccggcagc actggcggtc ccgccccaca ggggcactga gccgtatgcc ggtcgatcac
     1021 ccgcgcgcgc aggccgccgt gcgcgagttg ctctgtgcca tcggcgagga ccccgatcgg
     1081 gaaggcctac gcgagacacc ggccagggtg gctcgggctc tcggcgagtt cttcgcgggc
     1141 cttgaccagc gtccggagga catcctctcg acgaccttcg acatcgacca cgacgagatg
     1201 atcctcgtgc gcgacatcga ggtctacagc acgtgtgagc accacctcgt gcccttccac
     1261 ggggtcgccc atgtgggcta catccccgcc aaggacggga aggtcacggg gctgagcaag
     1321 atcgcccgcc tcgtcgacgt gttcgccaag cgtccgcagg tccaggagcg gctcacgacg
     1381 caggtcgccg atgccctcgt cgatcacctc ggcgtccagg gctgcatcgt cgtcctcgag
     1441 tgcgagcacc tgtgcatgtc catgcggggg gtcaagaaac ccggatcccg cacgatcacc
     1501 tcggcggtgc gcggccagtt gcgcaacccc gccacccgtg cggaggccat ggcgctgctg
     1561 ctcggcggga agcggtgacc gcccccgcac cctcgcagtg gctggcctcg gtgagcgggc
     1621 ggcatacccc tgggccgttc tccgacggcg cccgaacgtt ggtcatgggc gtcgtcaacg
     1681 tcacccccga ctcgttcagc gacggcggtc agtggttcga gcccgacgcg gcgatcacgc
     1741 acgcacgcgg cctgctcgcc gacggggccg acctgctcga catcgggggt gagtccaccc
     1801 ggccgggggc gtcgcgtccc agcgtccacg aggagttgag tcgggtgatt cccgtcgtgt
     1861 cggcgctggc cgccgaggga gcccgagtgt cgatcgacac gatgcgggcg gaggtcgcgg
     1921 gcgctgcgct cgccgccgga gcggcgctcg tcaacgatgt cagcgggggg ctcgccgacc
     1981 cgcagatgtt gccccttgtc gcggcggcgc aggttccgtt cgtcgtcatg cactggcgag
     2041 ggcacagcgt cgacatgcag gaccgcgcgg catacgccga cgtcgtcgcg gaggtatgcc
     2101 gcgagctcgc ctcccgtcgc gacgccgctc tcgccgcggg gatccgcgcc gaccggctcg
     2161 tgctcgaccc cgggctcggc ttcgccaagc tcgccgagca caactgggcg ctgctcgccg
     2221 gcctgccggc gctgcgcgac ctcgggcaac cgatcctgct cggggcctcc cgaaaggcct
     2281 tcctcgggaa ggtcgggcgg cccgagcacg cgaccccaag gccggccgcc gagcgcgacg
     2341 tcgccaccgc ggcgaccacg gtgctggcgg ggctcgctgg cgtgtgggcc gtgcgggtgc
     2401 acgacgtccg ctcgacgagg gacgcgttgg atgtcctcga agcgacccaa gcccacggag
     2461 gcaccccatg accgaccgca tcgtcgtcga aggcatccgg ggccttggct atcacggggt
     2521 gttcgagcac gagcgccgcg acgggcaaca gttcctcgtc gacgttgcca tcgagttgga
     2581 cctgcgtcgg cctgggcgct cggacaacct cgtcgacacg gttcactacg gcgaggttgc
     2641 tgctcaggtg ttggcccgca tcgaggggga gcccttcgac ctcatcgagg cgctcgccga
     2701 ggtcatcgcc gccgatgtcc tgagccttcc tggggttgaa gccgtcgatg tcgtcgtcca
     2761 caaaccgcag gcgccggtcg gggtcccggt caccgacgtc cgggtcgaga tccgtcggga
     2821 gcgtggcact cgggtggtca tcgcgttggg ctccaacgtg ggtgatcgag tggcgaccct
     2881 gaccgaggcc gtcgaggcgc tcgccgaggt gccgcggttg gccctcatcg cggtgtcgga
     2941 cctcgtcgag tcggaccctt tcggcggacc ggaccaaccg gcatacctca atggcgtggt
     3001 gctcgcgaac tttgccggct cacccgccac cctgctgcgg gccctgcacg cgatcgagca
     3061 ccgcaacggg cgcacgcgtg agatccgttg gggagcaagg acattggacc tcgatctggt
     3121 tcagttcgga gtgccgggga gtgatggcga gcggctgtcg gacacgcccg ctctgctgct
     3181 gccgcaccct cgggcgcacg agcgcaactt cgtcctgcgc ccgtgggcgc acctcgaccc
     3241 cgaggcaacc ctgcgggtcg ggcccaccgt cgcccacccc gtccgcctcg tggctgacgt
     3301 cctcgtcgag ttgggcctgt ccggcgtccg gccgggcccg gtctggagcc cggcatggtg
     3361 acctcgggag gcgtgcggct gcgtcagttg gtcgtcgtca cggtggtcgt cggcctgctc
     3421 ggcctggtcg tgttacggct gctcggtggc accgtcccca gcccggggtg gccgggagtg
     3481 gtcctcctgc tgttcatggc tgtcggcgtc tacttcgccg ccctgccggt caagcggctg
     3541 cgcgagcgtc gcgtgctctc tccgggcagt gccctgcgcg ccgcccgatc cctcgtcctg
     3601 gcgcaggcgg cggctttgac cggggccggc ttgctcggct ggtatgccgc gcaggtagtg
     3661 gtcctgctgc ccaacgtgga cgtcgactct caacgcggcc gtatgtggct cctcgtcgct
     3721 cacgcggtgg cagcgatcgt gctcgtggtg agtggccttc tcgcgcaacg gatttgtcgg
     3781 atcgacccac ccgacgccgg ggaccccggt gaccgagcga ccaaccctgg aggcgtggca
     3841 cccgcctgac gacccggtag gtcgggctca gtcagcgatc gcctggtggc gcctgaggcg
     3901 tctgcgctcc cggcgccgac gccgttgcgc gcgccagccg gccatccc
//
LOCUS       DAOMZY010000317         3920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_21959_length_3920_cov_5.336352, whole genome shotgun sequence.
ACCESSION   DAOMZY010000317 DAOMZY010000000
VERSION     DAOMZY010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21959_length_3920_cov_5.336352"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PLK64_14460"
     CDS             <1..512
                     /locus_tag="PLK64_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HPV80995.1"
                     /translation="RAEGLWVRAYVSMCFGDPWEGDVPVGQVVDAARRLMDLGCDELS
                     LGDTIGVATTGQVPALLDGLDAAGIGRERIAVHFHDTYGQALANTMAALRDGVTIVDS
                     SAGGLGGCPYAKSATGNLATEDLVWALHGAGVETGVDLEALVATSVWLAGLLGRPSAS
                     RVVTALAGS"
     gene            complement(681..2972)
                     /locus_tag="PLK64_14465"
     CDS             complement(681..2972)
                     /locus_tag="PLK64_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HPV80996.1"
                     /translation="MQRRRGTTPRAPHPAARSLADDLRHRTDEQLTTLLLARPDLARP
                     TPADLVALAARATTRTSVQRALERLDRGQLQTVEALVVAAEPNRTGTAAVEDVAALLG
                     GDPTEALDRLWSVGLVWWTSSGPHPVRAVTEVLGPHPGGLGLPAAEVSSEYAAHTPPL
                     PDDLPALLAEAPVAARAILDTLTWGPPIGAVPPSGPAHQGASWLVTHGLCAPSGIGHI
                     TLVREVGLALRGGRLHREPALQAPALTDAQVDPAVADRVAGAGARDLLGLLDQLGDLW
                     TALPPRVLRSGALGVRDLAGLARSLDVETAQAGWLAEVALAAGLIADDGELTPAFSPT
                     PEFDVWRSRSAGARWATVAQAWWESPRAAYLVGTAPNGGATVGALGPEVRWPAVRGVR
                     ADVLGELAGLPAGTAPSAESVIERLLWRRPLGDRRALTDAAEAVLREAEWLGARALGT
                     LTSLGRALVDRVPTAELAATADATLPASVDHVLLQADLTAIAPGPLDGALGRFMRLVS
                     DVESRGGATVLRLSGESVRRALDTGWTADEVLDTLRDSSRTPVPQPLDYLVRDVARRH
                     GQTRVGAATAYIRSDDEAVLAALLADRSLARAVLRRIAPTVLVSQADPATLVEVLRAG
                     GYSPARESFDGTVVVERPATRRARPPAARPAPEPTLSPVDAALVRSVVATLRNAPERP
                     VAGAAPPVTLSGSPQETSALLRQAVSDSLAVWIGYADSNGRTARHLVRPVRIDGGRVY
                     AVSGESDSEQLFMLHRITGATTG"
     gene            3104..3739
                     /locus_tag="PLK64_14470"
     CDS             3104..3739
                     /locus_tag="PLK64_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HPV80997.1"
                     /translation="MVFTGFPPQALDFYAGLEADNSKAFWTANKGTWESAARDPMLAL
                     LDELEDEFGPATPFRPYRDVRFSADKSPYKTHLGAVAGPTKGVGCYVQLSAEGLLVGG
                     GFHSHGSAQTARYRAAVDAPASGTALEAIVGGLRDKGFAVRGASVKTAPRGFAKDHPR
                     IELLRMAELMVIREIGTPEWLASPRVVTEVAEAWRAVGPLSDWVVEHVGAD"
BASE COUNT          569 a         1345 c         1490 g          516 t
ORIGIN      
        1 ctcgcgcgga gggcctgtgg gtgcgggcct acgtctcgat gtgtttcggc gacccgtggg
       61 agggcgatgt cccggtgggt caggtcgtcg acgccgctcg ccgcctcatg gacctcgggt
      121 gcgacgaact cagcctgggg gacaccatcg gggtggcgac cacgggccag gtgcccgcgc
      181 tgctcgacgg gctcgatgcg gccgggatcg gtcgcgagcg gatcgccgtg catttccacg
      241 acacttacgg gcaggcgttg gccaacacca tggcggcgct gcgcgacggt gtgacgatcg
      301 tcgactcgtc agccggtggg ctgggtggct gtccttatgc caagtcggcg accggcaacc
      361 tcgcgaccga ggacctcgtc tgggccctgc atggtgcggg tgtcgagacc ggggtcgacc
      421 tcgaagccct cgtcgcaacg agcgtgtggc tggctggact gctcgggcgt ccctccgcgt
      481 cgcgggtcgt caccgccctc gcggggtcct gacgccctgg gcgagaagcg gcaggagggg
      541 tgaaatttcc ccgtacttca ggcacggagt gagtacctga agtaccagtt cttctcaggg
      601 ggtatggggg tcggcccgcg gcctgaagtt gagcctttca gggcgtatgg gggcgccccg
      661 cggcagtgcc gagtgatcgg tcagccggtg gtggcgccgg tgatccggtg cagcatgaac
      721 aactgctcgg agtcggactc gccggacacg gcatacaccc ggccgccgtc gatgcgcaca
      781 gggcggacga ggtgacgtgc ggtgcggccg ttgctgtcgg catacccgat ccacaccgcg
      841 agcgagtcgg aaactgcttg gcgcagtagc gcgctcgtct cctgcggcga gccggacaac
      901 gtgaccggcg gtgccgctcc ggcgaccggc cgctcgggtg cgttgcgcag ggtggcgact
      961 acggagcgca ccagcgccgc gtcgaccggc gacagcgtgg gctcaggagc agggcgtgcg
     1021 gccggggggc gcgcccgccg cgtggccggg cgctcgacga ccaccgtccc gtcgaaggac
     1081 tcgcgcgccg gcgagtagcc gcccgcccgc agcacctcga cgagggtggc ggggtcggcc
     1141 tgcgagacga gcaccgtcgg ggcgatccgg cgcagcactg cgcgggccag gctgcggtcg
     1201 gccagcagcg ccgcaaggac cgcctcgtca tcagagcgga tgtatgccgt cgcggcgccg
     1261 acccgcgtct gcccatgccg ccgggcgacg tcgcggacga ggtagtccag cggttggggg
     1321 acgggcgtcc ggctggagtc gcgcaaggtg tcgagaacct cgtcggcggt ccacccggtg
     1381 tcgagggccc ggcgcaccga ctcgccggac agccgcagaa ccgttgcgcc accgcgggat
     1441 tcgacatcgg agaccaagcg catgaagcgg ccgagcgcgc cgtcgagcgg cccgggggcg
     1501 atcgcggtga ggtcggcctg caacagcacg tggtcgacgg acgccggaag cgtcgcatcg
     1561 gccgtcgccg ccaactcggc cgtcggcacc cgatcgacga gcgcccgacc gaggctcgtc
     1621 agggtgccca gggcccgagc cccgagccat tcggcctcgc gcagcaccgc ctcggcagcg
     1681 tcggtcaacg cccgtcggtc gcccaacggc cgacgccaga gcagccgctc gatcactgac
     1741 tccgcgctcg gtgcagtccc cgccggaagc ccggccaact cgccaagcac atccgcgcgc
     1801 acgccgcgca ccgccggcca gcggacctca ggcccgagtg cgcccacggt cgccccgccg
     1861 ttcggtgccg tccccacgag gtatgccgcg cgcggactct cccaccaggc ctgggcgacc
     1921 gtcgcccacc gggccccagc cgagcgtgag cgccagacgt cgaactcagg ggtcggcgag
     1981 aaggccgggg tgagctcgcc gtcgtcagcg atcagcccgg cggcgagcgc cacctcggcc
     2041 agccatccgg cctgggcggt ctcgacatcg agggagcgag ccaggccggc gaggtcgcgg
     2101 acgccgaggg cacccgagcg aagcactcgt ggcggcaaag ccgtccagag gtcaccgagt
     2161 tgatcgagca ggcccagcag atcacgcgcc ccggccccgg ccacccgatc ggcgacggcc
     2221 ggatcgacct gagcgtcggt cagcgcaggg gcctgcaacg cgggttcgcg atgcagccga
     2281 cccccacgca gggccaaccc cacctcgcgc acgagggtga tgtgcccgat cccgctcggc
     2341 gcacacagcc cgtgcgtgac cagccacgag gcaccctgat gagccggacc cgacggcgga
     2401 acggctccga tggggggacc ccacgtgagc gtgtcgagga ttgctcgggc tgccaccggc
     2461 gcctcggcga gcagggcggg aaggtcgtcg gggagcggag gggtgtgggc ggcatactcc
     2521 gaggagacct cggccgcggg gaggccgagc ccgcccgggt gtggcccgag cacttccgtc
     2581 acggcgcgca cggggtgcgg cccggaggag gtccaccaga cgaggccgac cgaccagagt
     2641 cggtcgagtg cctcggtggg gtcgcccccg aggagggccg cgacgtcctc gaccgcggcg
     2701 gtgccggtgc ggttcggctc ggcggcgacg acgagggcct cgacggtctg caactgaccg
     2761 cggtccaggc gctcaagagc ccgctggacg ctcgtgcggg tcgttgcccg agcggcgagc
     2821 gcgacgaggt cggccggagt cggtcgggcc aggtcggggc gggcaagcaa cagtgtcgtg
     2881 agttgctcgt cggtgcgatg acggaggtcg tcggccaggc tgcgcgccgc cggatgcggt
     2941 gcgcgtgggg tcgtacctcg cctccgctgc atcagatcat cgtcgcacgc cccgaccatc
     3001 gggtatgccg cccgcccacc cccagggacc gcgcggggag gccgcccgcc caccgtgcct
     3061 gctcggagtt gcctactcgg aaggtggctg tcagggtgcg gagatggtct tcacggggtt
     3121 cccgccacag gctctggact tctatgccgg gctggaggct gacaacagca aggcgttctg
     3181 gacggcgaac aagggcacct gggagtcggc cgcgcgcgac ccgatgctcg ccctgctgga
     3241 cgagttggag gacgagttcg gtccggcgac gccgtttcgg ccctatcggg acgtgcggtt
     3301 cagcgcagac aagtccccct acaagaccca tctcggagcg gtggccggac cgaccaaggg
     3361 ggtcgggtgt tatgtccagc tcagcgccga ggggttgctc gtcggcggag gcttccactc
     3421 acacggatcg gcacaaaccg ctcgctaccg ggctgcggtc gacgcgccgg cctcgggaac
     3481 ggcactggag gccatcgtcg ggggcctacg ggacaagggt tttgccgtgc ggggcgccag
     3541 cgtgaagacg gcgccgcgag ggttcgccaa agaccatccg cggatcgagc tgttgcggat
     3601 ggccgaattg atggtcatcc gggagatcgg cacgcccgag tggctggcca gcccccgggt
     3661 cgtcaccgag gtcgccgagg cctggcgcgc cgtcgggccc ttgtcggact gggttgtcga
     3721 gcacgtcgga gccgactgag cgcttgaccg ggctgaccgg ccgaggggcc gacgggctgc
     3781 tcatcgacat gggctgcgaa tcgacttgcc agccccatta ggcttggcct cgcgcgacgt
     3841 gcgcggatgg ccggacccgc cggcccacga gctcgaggtg agacggtgcc cacaggcaag
     3901 gtcaagtggt acgacgcgga
//
LOCUS       DAOMZY010000318         3919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_21965_length_3919_cov_5.565476, whole genome shotgun sequence.
ACCESSION   DAOMZY010000318 DAOMZY010000000
VERSION     DAOMZY010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21965_length_3919_cov_5.565476"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="PLK64_14475"
     CDS             complement(<1..1206)
                     /locus_tag="PLK64_14475"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPV80998.1"
                     /translation="MRLARVEISGYRRLVKASTNIDGRITAFVGSNEAGKTSIFNALS
                     WLSSGGALAVVAQSRSKRPAPPQHVVRAFFALAPNDLETLKDLDLAELPRTIVAFRTA
                     DGTLGHEIRPAPRRATKPFSDAAKRLASTATRLEGRIESVASDEVGPQEWFAHVKDAL
                     AEPDSQWGQEWIQAARSLEDWLREVPPGRSTKSRDAVLADALAQVRDLVTQPHPREAT
                     LARLSARVPEFVVFGDANRVLETVHEMSADQFRQNPPPALRDLLRIAGVTLTELWQHT
                     ASGDTSSRESLLEEGNARLLEFFSQSWNQSNVAVRLNTNSSQLEVLVKELRESGPVTQ
                     VSERSDGLRIFIALAAFLASGGWKVPPVLLIDEAETHLHFDAQADLVGVLLKPRSVSW
                     WVAREGRMWL"
     gene            complement(1262..2041)
                     /locus_tag="PLK64_14480"
     CDS             complement(1262..2041)
                     /locus_tag="PLK64_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV80999.1"
                     /translation="MIATQTAMPFDTIENDLVGRLIRTNAVEASVTELNAAVAVARAF
                     LAGAAVTEATPWRPEHGRLATARLTEWIASKQTSSPAREVREVRPVRWPEPSERFETR
                     PAADRQVVTSSREFVRGYPYAGYAKSVYEVNTFDAFSTEFILADLFEKSGSVRAWVRI
                     DETVPLRITYLAGAVQRQYEPDFIVIDDAGVHWIVEGKRDGEMTSPMVIAKRDAAAAW
                     VKTVNGSDEVHETWAYLLASESVCSAAGSWDGLKSGGQVHR"
     gene            complement(2094..>3919)
                     /locus_tag="PLK64_14485"
     CDS             complement(2094..>3919)
                     /locus_tag="PLK64_14485"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPV81000.1"
                     /translation="AYRRIDGRIGVVSIDYDPDPSVERTAYRRIDGRIGVVSIDYDPA
                     LVEQVSYNLDLREPNQQALDALAKALDTAEPGAEFIADLATGVGKTYIASGLLDYLYE
                     SGVRNVLIVTPGSTIQRKTIDNLTPGHPKYLRGLQSRPMVITLDTVERGEVGAALADP
                     DRFKVFVFTVQSLLRPNKDDRRRAFQPHETLGTAVRTYLESADDLVVIADEHHVYYSG
                     SAKKFEAAITELCPLATIGLTATPHAASESRVVYRYPLSEAIADGWVKIPVLVTRQDG
                     IHDVRTQLADGLTLLDAKREAMRAYCAQVRGRKFAEPVMFLVASTIDEATEYRDLLIG
                     SDMLGSAEQVLLVTSEEPDATLALLDRLEDPDSPVRAVVSVSILKEGWDVKNIYVIAS
                     VRSMESNLLTEQILGRGLRLPFGQRTGNPMLDTVEVLSHRSFASLLKQAKSLLEQTLG
                     DRVDEASVVVNPRLGQHASGAAVGEQGELPTDFAFPTSGTVEFELPGAAATDPNQDAL
                     FDVEDIDDGGESSRHTGLMVSTLDDRVGAGAASVETLRRVLRPRTPGGVTIPLFLPSV
                     VTRWERDPFTLASINLDSVEALGQTFAKDNAPTLTRKAYRR"
BASE COUNT          675 a         1317 c         1231 g          696 t
ORIGIN      
        1 cagccacatc cgaccctccc gggccaccca ccaacttacg gatcgaggct taagcagaac
       61 gccgaccaga tccgcttgcg cgtcaaagtg cagatgggtc tctgcctcgt cgatgagaag
      121 cactggggga accttccatc ctcccgaggc cagaaatgcg gctagggcga tgaagatcct
      181 gaggccgtcg ctcctctcgc tcacctgggt gactggccct gactcgcgca gctccttgac
      241 cagaacctca agttgcgagc tgttcgtgtt gagtcggacg gccacattgg actgattcca
      301 ggactggctg aagaactcca agagtcgggc gttgccctct tcgaggagcg actcacggga
      361 cgacgtgtcg ccactcgccg tgtgttgcca aagctccgtc aaggtcaccc cggcgatgcg
      421 taggaggtcg cgaagcgcag ggggagggtt ctgccgaaac tggtcggccg acatctcgtg
      481 gacggtctca agaactctgt tcgcgtcacc gaagacgacg aactcgggaa cgcgcgccga
      541 tagacgagcc agtgtcgcct cgcgcggatg cggttgggtt acaagatccc gaacctgcgc
      601 cagggcgtcg gcgagtactg cgtcacgaga cttggtcgac cgtcctggag gcacctcgcg
      661 aagccagtcc tcgaggctcc ttgctgcttg tatccactcc tggccccatt gagaatcggg
      721 ttcggccaga gcatccttga cgtgggcgaa ccattcctgc ggccccactt catccgacgc
      781 gacgctctct attctcccct cgagtcgcgt tgcggtgctg gccaggcgct tcgctgcatc
      841 gctgaacggc ttggtggcgc gtcgcggggc ggggcggatc tcatgcccaa gcgtcccgtc
      901 cgcggttcga aacgcgacga tcgtgcgcgg cagttccgca aggtcgagat ctttgagagt
      961 ctcgagatcg ttgggggcca gggcgaagaa ggccctaact acgtgctggg gtggagcggg
     1021 acgcttgctc ctgctctgtg cgaccaccgc aagcgcacca ccgctgctca accaactgag
     1081 ggcgttgaag atgctcgtct ttccggcctc gttcgagcca acgaaggcgg tgattcgtcc
     1141 gtcgatgttg gtggacgcct tcacgagacg tctgtagccg gagatctcca cgcgggcgag
     1201 tcgcatcaga tcccactccc tcgggagttg accttggcgc cgttggcgaa gtgtcttgtg
     1261 gtcatcggtg aacctgtcca ccggacttga gcccgtccca tgagccggcg gcgctgcaca
     1321 ccgattcgga ggcgagcagg taggcccagg tctcgtggac ctcgtcggag ccgttgactg
     1381 tctttaccca cgccgccgcc gcgtcgcgct tggcgatgac catgggcgag gtcatctcgc
     1441 cgtcgcgctt accctcaacg atccagtgca cgcccgcgtc gtcgatgacg atgaagtcgg
     1501 gttcgtactg tcgctgcacc gcgccagcga ggtaggtgat ccgcaaaggc acggtctcgt
     1561 cgatccgcac ccacgcccgc accgagccgg acttctcgaa caggtcggcc aggatgaact
     1621 cggtcgagaa ggcgtcgaag gtgttgacct cgtacaccga cttcgcgtac ccggcatacg
     1681 ggtagccgcg gacgaactct cgtgatgacg tgacgacctg ccggtccgcc gcaggccggg
     1741 tctcgaaccg ctccgacggc tcgggccagc gcacaggccg aacctcgcgg acctccctcg
     1801 ccggcgagga ggtctgcttg gacgcgatcc actcggtcag acgcgcggtc gcgagccggc
     1861 catgctcggg ccgccacggt gtcgcctcgg tgaccgcagc gccggccagg aacgcccggg
     1921 ctacagcgac cgcggcgttg agctcggtca ccgacgcctc gacggcgttc gtccggatga
     1981 ggcggccgac gaggtcgttc tcgatcgtgt cgaacggcat cgccgtctga gtcgcgatca
     2041 cctgctcgac ctgttcgcgg ataacgacat gcgcctcacc cgatgaatca cgctcagcgt
     2101 cgataggctt tgcgcgtcaa cgtcggagcg ttgtccttgg cgaaggtctg gccgagcgct
     2161 tcgaccgagt cgaggttgat cgaggccagt gtgaacgggt ctcgctccca ccgcgtcacc
     2221 accgacggca ggaacaaggg gatcgtcacg ccaccaggcg tccgcggcct cagcacccgc
     2281 cgcagcgtct ccaccgatgc cgcgcccgcg ccgacgcggt catcgagcgt ggagaccatc
     2341 agtcccgtat gccgtgagga ctcccccccg tcgtcaatgt cctcgacgtc gaacagagcg
     2401 tcctgattcg gatctgtggc cgccgcgccc ggcagctcga actcgactgt ccccgacgtg
     2461 gggaacgcga agtctgtcgg aagctcgccc tgctccccga cggcagcacc agatgcgtgt
     2521 tggccgagac ggggattgac gacgacggac gcctcgtcca cccggtcacc aagggtttgt
     2581 tcaagcagcg acttggcctg cttcaacagc gacgcgaagg atcggtgcga gaggacctcg
     2641 accgtatcga gcatcgggtt gccggtccgc tgtccgaacg gcagccgcag cccgcggccg
     2701 aggatctgct cggtgagcag gttggactcc atcgaccgga cagaggcgat gacgtagatg
     2761 ttcttgacgt cccacccctc cttgagtatc gaaaccgaca ccacagcacg caccggcgag
     2821 tccgggtcct cgagtcgatc gagcaaagcc aatgtggcgt ccggctcctc tgacgtcacg
     2881 aggagcacct gctctgccga ccccaacatg tccgaaccga tcaacaggtc gcgatactcc
     2941 gtcgcctcat cgatcgtcga cgcgacaagg aacatcaccg gctcggcgaa cttccgcccc
     3001 cgcacctgtg cgcagtaggc cctcatcgcc tcccgcttcg cgtcgagcag cgtcagaccg
     3061 tccgcgagct gggtccggac gtcatggatg ccgtcctgcc tcgtcacgag cacggggatc
     3121 ttcacccacc catcggcgat cgcctctgac aacggatagc ggtagacgac cctcgactcc
     3181 gacgctgcgt gcggagtggc cgtcagtccg atggtcgcca gcgggcacag ctcggtgatc
     3241 gccgcctcga acttcttcgc cgaacccgag tagtagacgt gatgctcatc cgcgataact
     3301 accaggtcat cggcggactc caagtaggtc cgcaccgccg tcccgagcgt ctcgtgcggc
     3361 tggaacgccc gccgccgatc gtccttgttg ggccgcaaca gcgactgcac cgtgaaaacg
     3421 aagaccttga accgatccgg gtccgccaac gccgcaccga cctcgccgcg ctcgacagtg
     3481 tcgagagtga tgaccatggg acgcgactgc aggccgcgca ggtacttcgg atgccccggc
     3541 gtcaggttgt cgatcgtctt gcgctggatc gtcgaccccg gcgtcacgat caggacgttc
     3601 cgcacgcctg actcgtagag gtagtcgagc agaccgctgg cgatgtaggt cttgcccacc
     3661 ccagtcgcca ggtcagcgat gaactccgca ccgggctcgg cggtgtccag cgccttggcc
     3721 aaggcatcga gggcctgctg gttcggctcg cgaaggtcca ggttgtaact cacctgctcg
     3781 acgagggccg ggtcgtagtc gatgctcacg accccgatcc ttccgtcgat tcttcggtat
     3841 gccgtgcgct cgacggaagg atcggggtcg tagtcgatgc tcacgacccc gatccttccg
     3901 tcgattcttc ggtatgccg
//
LOCUS       DAOMZY010000319         3914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_22004_length_3914_cov_5.384296, whole genome shotgun sequence.
ACCESSION   DAOMZY010000319 DAOMZY010000000
VERSION     DAOMZY010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22004_length_3914_cov_5.384296"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="PLK64_14490"
     CDS             <1..722
                     /locus_tag="PLK64_14490"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145733.1"
                     /note="catalyzes the reversible transfer of the terminal
                     phosphate of ATP to form a long chain polyphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HPV81001.1"
                     /translation="VGTGNYNPKTARLYEDLGILTCDPQVGEDLSRLFNQLSGIAPRS
                     RFRRLLVAPRTVRSGLIELVDAEIERHEADGGGAIRLKMNSIVDEQLIDALYRASQAG
                     VPVDVWVRGICAIRPQVPGLSDNLRVRSTLGRFLEHSRIFWFAGGGSPQVYIGSADMM
                     HRNLDRRVEALVQIVDTSHVAELTNMFNMGLADTTSRFELGADGTWTRVYRDADGQPL
                     NDLQTLMWDHHNKARRKARRR"
     gene            731..1693
                     /locus_tag="PLK64_14495"
     CDS             731..1693
                     /locus_tag="PLK64_14495"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPV81002.1"
                     /translation="MSTINAAGTVPWRVNGDALEVALVHRPRYDDWSWSKGKLEPGER
                     WPVAAFRETQEETGLQVRLGMPLPPTTYRVLDRDGVPATKWVRYWAAQVTGGDGSLTH
                     EIDEVAWLDATSADTRLDYSHDREQLRALLRAYQRGLLDTWPLLVVRHALATPRSAWK
                     GDDRRRPLSPAGHVQVPLITDTLAAYGVEALTTSPSTRCVQTLAGYAKRSGLDPHMRN
                     AISEEGFAKRGAKRVYRVVDEVLATGVPTAVCSHGPVLPTILEALVGRLAPVDELIPG
                     ADTSLAEAADLGIEKGEVFIAHVCGVGDAARIIAVERITSKAEV"
     gene            1910..2998
                     /gene="pstS"
                     /locus_tag="PLK64_14500"
     CDS             1910..2998
                     /gene="pstS"
                     /locus_tag="PLK64_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HPV81003.1"
                     /translation="MTTRSSAIIIVAMSGALALGACGSDATKTGTAATGTAASDCPTG
                     TLKAEGSSAQKNAIEAAIKSFQTTCSGTTVNYAATGSGAGIKQFIAGQVDFGGSDSTL
                     KDDEAASAATTCASPAWHLPMVTGPVGIAYNLPSVSSLVLNADVTAKIFLGQITMWND
                     PAIAALNSGTTLPATKISVFFRSDESGTTDNFTKYLKAAAGTAWTVESGKKWNGTVGE
                     GKPQNAGVATAAKSVEGGISYMEWSYVTQNKLGLAKIDNGSGPVELTSASVGKMIETA
                     TQKGTGNDLSLTLDYATKTPGAYPINLVTYEIVCSKYKDAAKGKLVKSFLTHFVSADV
                     QKSLEALGYAALPSSVSAKITAAVAAIG"
     gene            3053..>3914
                     /gene="pstC"
                     /locus_tag="PLK64_14505"
     CDS             3053..>3914
                     /gene="pstC"
                     /locus_tag="PLK64_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPV81004.1"
                     /translation="MTEPAGSSWTATVTAPRRGGDRWFGLASHGAGVAVVALVTLVGV
                     FLVSQAIPALSKNEANFLTSREWSVGGDNPRFGIMELLWTTTTASIIAMAIAVPIGVC
                     VALFITEYAPQWLRRPAATLVDLLAAVPSIVYGLWGLIIFRPVVRPLEDWLSTRLGWL
                     PLFAPTGITGGTIMFIGVVLAIMILPIVTAISREVFAQTPTTHKEGALALGATRWEMI
                     RTAVLPFGKPGVISAAMLALGRALGETIAVTFIVSTLAAGSDWTWSMLSGGETFASRI
                     ANNAAEFDSPQ"
BASE COUNT          647 a         1379 c         1257 g          631 t
ORIGIN      
        1 acgtcggcac cggcaactac aaccccaaga cggcccgcct ctacgaggac ctcggcatcc
       61 tcacctgcga cccgcaggtc ggcgaggacc tctcgcggct gttcaaccag ctctcgggca
      121 tcgcgccgcg cagccgtttc cggcggttgc tcgtggcgcc gcggacggtg cgttccgggc
      181 tcatcgagtt ggtcgacgcc gagattgagc ggcacgaggc cgatggcggc ggcgccatcc
      241 ggctgaagat gaactccatc gtcgacgagc aactcatcga tgccctctac cgcgcgtcgc
      301 aagccggcgt ccccgtcgac gtctgggtcc gtgggatctg cgccatccgg ccgcaggtgc
      361 ccggcctcag cgacaaccta cgggtccgtt ccacgctcgg gcgattcctg gaacactcgc
      421 gaatcttctg gttcgccggt ggcgggagcc cgcaggtcta tatcggcagc gccgacatga
      481 tgcaccgcaa cctcgaccgt cgcgtcgagg ccttggtgca gatcgtcgac acgagccacg
      541 tcgccgagct gaccaacatg ttcaacatgg ggctggccga caccacctcc cgcttcgagc
      601 tcggtgccga cggcacgtgg acccgggtct accgggatgc cgacggccag ccgctcaacg
      661 acctgcagac cctcatgtgg gaccaccaca acaaggcccg ccgcaaagcc cggcgtcggt
      721 agagccgtcg atgtccacca tcaacgctgc ggggacggtc ccgtggcgcg tcaacggtga
      781 tgccctggaa gtggcgctcg tccaccgacc ccgctacgac gactggtcgt ggtccaaggg
      841 caagctcgag ccgggtgagc gctggccggt tgctgcgttc cgcgaaacgc aggaggagac
      901 ggggctgcag gtccgtctgg gtatgccgct ccccccgacc acctatcgcg tcctggatcg
      961 cgacggcgtc cccgccacca agtgggtccg ctattgggca gcccaggtca ccggtggaga
     1021 cggcagcctc acccacgaga tcgacgaggt ggcttggctc gacgcgacgt cggccgacac
     1081 ccgactggac tactcacacg accgcgaaca gttgcgggcg ctgcttcggg cctatcagcg
     1141 cgggttgctc gacacctggc cgctgctcgt cgtgcggcat gccctggcga ccccacggtc
     1201 ggcgtggaag ggcgatgacc gcaggcgtcc gttgtccccc gcggggcacg tccaggtgcc
     1261 gctcatcacc gacacgttgg cggcatacgg cgtcgaagcg ctgacgacct caccgtcgac
     1321 ccgatgcgtg cagaccctcg ccggatatgc caagcgcagc ggcctggacc cgcacatgcg
     1381 caacgccatt tccgaggaag gcttcgccaa gcgcggcgcg aagcgggtct accgggtcgt
     1441 ggatgaggtg ctggccaccg gagttcctac ggctgtgtgc agccacgggc cggtgctccc
     1501 cacgatcctc gaagccctcg tgggccgcct ggcacccgtt gacgagttga tccccggcgc
     1561 cgacacctcc ctcgccgagg ccgccgacct cgggatcgag aagggcgagg tgttcatcgc
     1621 ccacgtctgt ggtgtgggcg atgcagcgcg gatcatcgct gttgagcgaa tcacctccaa
     1681 ggccgaggtc tagccagcgc gcgaactgta gatgaccgtg tggcgaactt cgggttcgcc
     1741 accgaattgg cctgcaaacg ttcatgtttc gttcaacgcg acagcgcctg gcgtcatctg
     1801 acgtccctag ggtcgggcac ggagggcgga cgatgcggtc gcctccgaat caaccgaatc
     1861 cagccgaggc agaccgtctc gctgaccctg tgagagggac acccccgtga tgaccacccg
     1921 aagcagcgcc atcatcatcg tcgcgatgtc cggggccttg gccctgggcg cttgtggctc
     1981 cgacgccacg aagactggca ccgcggccac cggcaccgct gcgtccgact gcccgacggg
     2041 caccctcaag gccgagggat cctcggccca gaagaacgcc atcgaagcag cgatcaagag
     2101 cttccagacc acgtgcagcg gcaccaccgt caactacgcg gccaccggtt ccggtgccgg
     2161 catcaagcag ttcatcgccg gtcaggtcga cttcggcggc tccgactcca cgctcaagga
     2221 cgacgaggcg gcttccgctg cgacgacctg cgcctccccg gcctggcacc tgccgatggt
     2281 gaccggtccg gtcggcatcg cctacaacct gccgtcggtc tcctcgctcg tcctcaacgc
     2341 cgacgtcacc gcgaagatct tcctcggcca gatcaccatg tggaacgacc cggcgatcgc
     2401 cgcgctcaac tctggtacga ccctgccggc gacgaagatc agcgtcttct tccgctcgga
     2461 tgagtcggga acgaccgaca acttcacgaa gtacctcaag gccgctgcgg gcaccgcctg
     2521 gacggtcgag tcgggcaaga agtggaacgg aacggtcggc gagggcaagc cgcagaacgc
     2581 cggggtggcc acggccgcca agagcgtcga gggcggcatc tcctacatgg agtggtccta
     2641 cgtcacccag aacaagctcg gcctagccaa gatcgacaac gggtccggtc cggtggagct
     2701 caccagcgca tcggtgggca agatgatcga gacggccacc cagaagggga ccggcaacga
     2761 cctctcgctc acgctggact acgccaccaa gacccccggg gcctacccga tcaacctggt
     2821 gacctacgag atcgtctgct cgaagtacaa ggacgcggcc aagggcaagc tggtgaagtc
     2881 gttccttacg cacttcgtct cggccgacgt gcagaagtcg cttgaggccc tgggctacgc
     2941 agcgctcccc tcctcggtct ccgccaagat cacggccgcc gtcgcggcta ttggctgagg
     3001 ctgcggattt cttcaggaca ttgacgtgtc tgcaaccaca actccaccag agttgaccga
     3061 accagcgggt tcctcgtgga ccgccaccgt cacggcccct cgacggggtg gggaccgctg
     3121 gttcgggctg gcctcgcacg gggccggcgt ggcggtggtg gccctcgtga cgctcgtcgg
     3181 cgtgttcctg gtctcgcagg ccatccccgc gttgtcgaag aacgaggcca acttcctcac
     3241 ctcccgtgag tggtcggtcg gcggggacaa cccgcggttc ggcatcatgg agttgctctg
     3301 gacgacgacg acggcgtcga tcatcgcgat ggccatcgcc gtccccattg gggtgtgcgt
     3361 ggcgctgttc atcaccgaat acgcgccgca gtggctgcgg cggcccgctg cgaccctggt
     3421 cgatctgctc gcagcagtcc cctcgatcgt ctacggcctc tgggggctca tcatcttccg
     3481 cccggtcgtg cggccgctcg aggactggct ctcgacccgt ctcgggtggc tgccgctgtt
     3541 cgcaccgacc ggaatcaccg gcggcacgat catgttcatc ggcgtggtcc tggccatcat
     3601 gatcctgccc atcgtcaccg cgatctcccg agaggtgttc gcccaaacgc ccaccactca
     3661 caaggagggc gcgctggcgc tgggggccac gcggtgggag atgatccgca ccgctgtcct
     3721 gccgttcggc aagcccggcg ttatcagcgc cgccatgttg gccctgggcc gtgccctcgg
     3781 cgagacgatc gccgtcacgt tcatcgtgtc gacgttggcg gccggctccg actggacctg
     3841 gtcgatgctc agcggcgggg agacgttcgc ctcccgcatc gccaacaacg ccgccgagtt
     3901 cgactcgccg caga
//
LOCUS       DAOMZY010000320         3913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_22015_length_3913_cov_4.811820, whole genome shotgun sequence.
ACCESSION   DAOMZY010000320 DAOMZY010000000
VERSION     DAOMZY010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22015_length_3913_cov_4.811820"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..148)
                     /locus_tag="PLK64_14510"
     tRNA            complement(76..148)
                     /locus_tag="PLK64_14510"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(113..115),aa:Gly,seq:gcc)
     gene            complement(266..1354)
                     /locus_tag="PLK64_14515"
     CDS             complement(266..1354)
                     /locus_tag="PLK64_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HPV81005.1"
                     /translation="MSKSKSKGAKKAAKKKESDKAAKAVKAVKVSKATKGSGKGASKS
                     KASKRGSSKAEPLASSPAALAAPNVYDVPVDVEPVEKKLRPDEPITSVLRVRRGFVLA
                     DFDPESTPGFDEGRKEGEEALSAFAPEMGAWQERLFAETKGGGKRSLLIVLQGLDSAG
                     KGGIVRHVMSNCDPAGIKATAFKAPTEEERQHDFLWRVRKALPGPGQIGVFDRSHYED
                     VLITKVRKLVPAGEITKRYAVINAFEREVIDSGTEILKVFMYISRDEQKARLMERLDR
                     PDKRFKYTPGDTDNRAYWDDYMKAFQVMLARTSTVAAPWYVIPANNKWYARYAVQQLI
                     LEKLRAMSPDWPVPDYDIEVEKARLADS"
     gene            1440..2198
                     /locus_tag="PLK64_14520"
     CDS             1440..2198
                     /locus_tag="PLK64_14520"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HPV81006.1"
                     /translation="MDLLIAPPEIQRHHPVGSRLDLRGLAAAYARPTGAQRWVSVNMV
                     STIDGAATGADGVTGSINSPADKIVFDLLRALSDIVVIGAGTARTEGYGPLTLDPEYA
                     ALRADLGLAPTLPLALVTRSGDIPDRLRQSAPGSVMVITAASCPALAHLRNDLGEEHV
                     IVAGEAEVDLAGAANTLARRGFQRIHSEGGPRLLGDLLRADVVDELCLTLAPHLVGGR
                     HKRIVEADDLRVSLVPHVLLHADGDLIGRWLVSR"
     gene            2258..3319
                     /locus_tag="PLK64_14525"
     CDS             2258..3319
                     /locus_tag="PLK64_14525"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HPV81007.1"
                     /translation="MRQPIDLPVQPMLAKAVAEVPVGDNLAYEPKWDGFRCIVLRDGD
                     DIELASRGTKPLTRYFPEVVEHVRRLLPERCAVDTEIVVRSGGPGAERLDWERLSQRI
                     HPAESRVRKLSVETPAELVCFDLLAEGDESLLGMPFGQRRARLEAALGHLADTDPIHL
                     TRMTTDPAIARDWFERFEGAGLDGVVAKPLDAAYAPGKRTMLKVKHKRTADAVVFGYR
                     VHTSGQGVGSLLLGLYGEWNGERLLIPVGGIAAFPMATRRALIDDFAPLVVLDDEGQA
                     RQAATERSRFSSNKDSSFVPVRPERVVEVAFDQMEGHRFRHAVTFLRWRPDRDPQSCE
                     LDQVDRAPAYDLGEVLTVG"
     gene            complement(3353..>3913)
                     /locus_tag="PLK64_14530"
     CDS             complement(3353..>3913)
                     /locus_tag="PLK64_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018770985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HPV81008.1"
                     /translation="GSRGVHVFCPIEPAREFLDVRHGVIAIARELERRMPDEVTTAWW
                     KEERGQRIFVDYNQACRDRTIAAAYSPRPLPGAPVSMPVSWESLRDIVPGDFTVRTVP
                     GLVGAQPDPWTAYDDAVGDLGVAMSWWERDVAEGLPEMAFPPDYPKMPGEPPRVQPSK
                     KRYEDEDYVPGGTGYLRAHPERPPRT"
BASE COUNT          598 a         1417 c         1252 g          646 t
ORIGIN      
        1 tgaacccgtg tacgcggctt tgcaggccgc tgcctcgcct ctcggccact ccaccgtgaa
       61 ggcggtctac ctccgagcgg acgacgaggc tcgaactcgc gacctcaacc ttggcaaggt
      121 tgcgctctac caactgagct acgtccgcag gaacaccacc ggtgtaactt ccccgtctgg
      181 tgcgaaagta gacactagcg gacggaccgg ggcgtgtcca aaccgccccg atccagcccg
      241 ccaggcctcg ccctgaccga ccggctcagc tgtccgcgag ccgggccttc tcgacctcga
      301 tgtcgtagtc cggcaccggc cagtctgggc tcatcgcccg caacttctcg aggatcagct
      361 gctgcacggc ataccgcgcg taccacttgt tgttcgccgg gatgacgtac cacggggcgg
      421 ccacggtcga ggtgcgcgcg agcatcacct ggaacgcctt catgtagtcg tcccagtagg
      481 cacggttgtc ggtgtcgccc ggcgtgtact tgaacctctt gtcggggcgg tccagccgct
      541 ccatgagccg ggccttctgc tcgtcgcgag agatgtacat gaacaccttg aggatctcgg
      601 tgcccgagtc gatcacctcg cgctcgaagg cgttgatgac cgcgtagcgc ttggtgatct
      661 cgcccgcggg gacgagcttg cgcaccttgg tgatcagcac gtcctcgtag tgcgaacggt
      721 cgaagacgcc gatctgccca ggccccggca gggccttacg gacgcgccac aagaagtcgt
      781 gctggcgctc ctcctcggtg ggcgccttga acgcggtggc cttgatgccg gccgggtcgc
      841 agttgctcat cacgtgccgg acgatgccgc ccttgcctgc gctgtccagg ccctgcagga
      901 cgatgagcag gctgcgcttg ccgccgccct tggtctcggc gaagagccgc tcctgccacg
      961 cgcccatctc cggggcgaag gcggacagtg cctcctcacc ctccttgcgg ccttcatcga
     1021 aaccgggagt cgactcgggg tcgaagtcgg ccagcacgaa gccgcgacgc acgcgaagca
     1081 ccgaggtgat cggctcgtcg gggcgcagct tcttctcgac cggctcgacg tcgaccggca
     1141 cgtcgtagac gttcggcgcg gccagcgcag cgggagagga ggcaagtggt tcggccttcg
     1201 aagagccccg cttggacgcc ttgctcttgc tggcgccttt acccgagccc ttggtggcct
     1261 tggagacctt gacggccttg accgccttag cggccttgtc ggattccttc ttcttggctg
     1321 ccttcttggc gcccttgctc ttgctctttg acatgcagac atcttgccgt aacgtccccg
     1381 tcagttcgag ggcaccttcg cgaacacgcc gacacgggct cggcccgcat acttcggtga
     1441 tggacctgct catcgcgccc ccggaaatcc agcgtcatca tccggtcggg agccggctgg
     1501 atctgcgagg tctcgccgcg gcatacgccc gaccgaccgg cgcacaacgc tgggtgagcg
     1561 tcaacatggt gagcaccatc gacggcgcgg cgaccggcgc cgacggcgtc acgggatcga
     1621 tcaacagccc cgctgacaag atcgttttcg acctgctgcg cgcgctctcg gacatcgtcg
     1681 tcatcggcgc ggggacggcg cgcacggagg gttatggccc gctcaccctc gacccggagt
     1741 atgccgcgct gcgcgccgat ctcggcctcg cgccgacgct accgctcgcc ctggtgaccc
     1801 ggtcgggaga catccctgat cggttgcgac agagcgcacc cggcagcgtc atggtcatca
     1861 ccgccgcctc gtgccctgcc ctcgcgcacc tgcgcaacga cctcggtgag gaacacgtca
     1921 tcgtggcggg tgaggccgaa gttgaccttg caggagccgc aaacaccttg gcgcgcaggg
     1981 gtttccaacg catccactca gagggcggcc cgcgcctgct cggcgacttg ctgcgggccg
     2041 acgtcgtcga cgaactgtgc ctgacgctgg caccgcacct cgtcggtggc cgacacaagc
     2101 gcatcgtcga ggccgacgat ctgcgcgtct cgctcgtccc gcatgtcctg ctgcacgccg
     2161 acggcgacct catcgggcgg tggctcgtca gccgctgacc cggcaagcct ggccgtcgct
     2221 cacgaccgac cctcgcgtca tggcgcacaa tggcccgatg cgccagccca tcgatctccc
     2281 cgtccagccg atgcttgcca aggcggtcgc cgaggtgccg gtcggcgaca acctggccta
     2341 cgagcccaag tgggacgggt tccgctgcat cgttctgcgc gacggcgacg acatcgagtt
     2401 ggccagccgc ggaaccaaac cactcacccg ctacttcccc gaggtcgtcg agcacgtccg
     2461 ccggttgctg ccggagcgct gcgcggtcga caccgagatc gtcgtgcgct ccggaggccc
     2521 tggcgccgaa cgcctcgatt gggaacgcct gtcccaacgc atccatccgg ccgaatcacg
     2581 ggttcgcaaa ctgtccgtcg aaacccccgc tgagttggtc tgtttcgacc tcctcgccga
     2641 aggcgacgag agcctgctcg gtatgccgtt cggccagcgc cgcgcgcgcc tcgaggctgc
     2701 gttgggccac ctcgccgaca ccgacccgat ccacctcacg cggatgacaa ccgaccccgc
     2761 gatcgcccgc gactggttcg aacggttcga gggtgccggc ctggacggag tcgtcgcgaa
     2821 gccgctcgac gcggcatacg ccccggggaa gcgcaccatg ctcaaggtga agcacaagcg
     2881 cacggccgac gcggtggtct tcggataccg cgtccacacc tcagggcagg gggtggggtc
     2941 actcctgctg gggctctatg gcgagtggaa cggcgagcgc ctgctcatcc ccgtcggcgg
     3001 gatcgcggcc ttcccgatgg ccactcggcg cgcgctgatc gacgacttcg ccccgctcgt
     3061 cgtcctcgac gacgagggcc aggcccggca ggcggcaacc gagcggtcac gcttctcgtc
     3121 caacaaggac agctccttcg tgccggtgcg ccccgaacgg gtcgtcgagg tggccttcga
     3181 ccagatggag ggacaccggt tccggcacgc ggtgacgttc ctgcgctggc gacccgaccg
     3241 cgacccgcag tcctgcgaac tggaccaggt cgaccgggcc ccggcctacg acctcggcga
     3301 ggtgctcacc gtcggctgac ggccgaggtc tgcccacgcg gagccaacgc cgttaggtgc
     3361 gcggcggtcg ctcgggatgc gcgcgcaggt agcccgtgcc gcccggcacg tagtcctcgt
     3421 cttcgtaacg cttcttgctc ggttgcaccc gcggcggctc ccccggcatc ttcggatagt
     3481 ccggcggaaa cgccatctcg ggcaacccct cagcgacgtc acgttcccac cacgacatcg
     3541 ccactccgag gtcccccacc gcgtcgtcgt atgccgtcca cggatccggc tgcgcgccaa
     3601 caagacccgg aacggttcgc accgtgaagt caccaggaac gatgtcccgc aacgactccc
     3661 acgacaccgg catcgacacc ggcgcaccgg ggaggggccg cgggctgtag gcagcagcga
     3721 tggtccggtc ccggcaggcc tggttgtagt cgacgaagat ccgttggccc cgctcttcct
     3781 tccaccacgc ggtggtcacc tcgtcgggca tccggcgctc caactcgcgg gcgatcgcga
     3841 tcaccccgtg gcgcacgtcg aggaactccc gcgctggctc gatcgggcag aagacgtgga
     3901 cgccccggct acc
//
LOCUS       DAOMZY010000321         3911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_22039_length_3911_cov_5.720954, whole genome shotgun sequence.
ACCESSION   DAOMZY010000321 DAOMZY010000000
VERSION     DAOMZY010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22039_length_3911_cov_5.720954"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="PLK64_14535"
     CDS             <1..1047
                     /locus_tag="PLK64_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81009.1"
                     /translation="GGHLLSPVQIALGTVITDPRMMNGALMSVTNPFGDNQAGLSGVH
                     TNWADPSAGPFMATSLWAALHQEAMLQPKTAAAVIAAFGTFIDGQSKQAQHMGYLYNP
                     ATGIFDLPNTGITSVQLFQAEAARHFLGTNLQADLDGLQKALEAELAQLGKDKEQAKQ
                     ILGQLVDWARDPKLIVSDVTDKLVDFAIDKAVDWTFPTTKDIEAQYEARIQSLSSSLD
                     SPIVKPSVWTDINMIATQLVVDLGTDRGPTTVVTAGGADGHPTPTSHNGDPTAYVGHV
                     SIYVPGDPAHPITDDFLARDSAGKATGVLNISQMNPYQRQAYLNWLLDPAVQNFLGTR
                     IDALKVAAGLAAGR"
     gene            complement(1059..2114)
                     /locus_tag="PLK64_14540"
     CDS             complement(1059..2114)
                     /locus_tag="PLK64_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81010.1"
                     /translation="MPRIFRGVLVALATVVVGVSGCTTTPSGPPQTTITWPQETSDAL
                     RLLLVGSGSTLYAVWGDRSVVIAGPQGTPTPTRLPKDDQQPGSETPQQIAVDPSGQVW
                     LIRSNQQVTTFLGVDHPASAAVSELGRGSITLPTGRVVPDRLVSEDGLAILDGTTLLA
                     STYTGGRTLNPDQDVLINRVTASGHDEVAGRWRTNPPDPLSGVPEGTSVPAGSVDLGV
                     VRRVAALAPDRIAFVVGTVSGTIKRDSRLSLAVLEAGTVRRVPIPGLCPSDYDATPVR
                     LSDRVVLLGAPAPRDNGECRSVSAARTWLRVDVVDGTSQVLATDTETLAVVGDRIVTA
                     TTTHVVDTWTTRITWGS"
     gene            complement(2150..2542)
                     /locus_tag="PLK64_14545"
     CDS             complement(2150..2542)
                     /locus_tag="PLK64_14545"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HPV81011.1"
                     /translation="MPSPDWVALQRAAIEVRDTAYAPYSGFPVGVAGLVDDGRVVVGC
                     NVENASYGVGLCAECGMVSALVAGGGGRLVAVSCVGPEGDTLMPCGRCRQLLWEHGGP
                     DCLVATSTGPRPMSELLPDAFGPANLGR"
     gene            complement(2590..3855)
                     /locus_tag="PLK64_14550"
     CDS             complement(2590..3855)
                     /locus_tag="PLK64_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV81012.1"
                     /translation="MSTSTASAPTATTVARRARRNRLLLLAGLGLVALSITRVISGEG
                     VLTASGTINAMVWLTLPVALAGLGGLISERAGVVNIGLEGMMMLGVWGAGFAGWRWGP
                     LAALVAALIGGALGGLLHALATVTFGVDHIVSGVAINLLAAGTVRFLSELTFTPSTGG
                     GPTQSPPMSGNIPTFDIPVLASGPDVLGSVERTGIPVLADAAGVLRGLMVGITPYELL
                     GLAMFPLTAYLLWRTALGLRLRSAGENPWAAESLGVNVVRVKYLAVVWGGALAGLGGL
                     FLVMFTGSYREGMTNGRGYIGLAAMIFGNWRPGGLAAGATLFGYADAVRLRSQNSLAV
                     LALFIFAGIVVIALGVLWLRRGRRLPGAIFIGIGVTFLLGYLGLDSLPNEVTTLTPYI
                     VTLVVLSVSSQNLRMPKADGMVYRKGEGH"
BASE COUNT          726 a         1314 c         1321 g          550 t
ORIGIN      
        1 ggtgggcacc tgctctcacc ggtccagatt gccttgggga ccgtcatcac cgacccgcgg
       61 atgatgaacg gcgcccttat gtcggtgacc aacccgttcg gcgacaacca agccgggttg
      121 tcgggcgtgc acacgaactg ggcagatccg tcggctgggc ccttcatggc cacgtccctc
      181 tgggccgcgt tacatcaaga agccatgctg cagcccaaga cggcagctgc cgtcatcgcg
      241 gcgttcggga cgttcatcga cgggcagagc aaacaagcgc agcacatggg ctacctctac
      301 aaccccgcga cagggatatt cgacctcccc aacaccggaa tcacgtcagt gcagttgttc
      361 caggcggagg cggcccggca cttcctcggc acgaacctgc aggcggacct cgacggattg
      421 cagaaggccc tagaggccga gcttgcccaa ctcggtaagg acaaggagca ggccaaacag
      481 atcctcggtc agctcgtgga ctgggcacgg gatcccaagt tgatcgtctc tgacgtcaca
      541 gacaaactcg tggacttcgc catcgacaag gcggtcgact ggaccttccc aacgaccaag
      601 gacattgagg cccagtacga agcccggatc caatctctat ccagcagcct tgactccccg
      661 atcgtgaagc cctcggtctg gacagacatc aacatgattg ccacgcaact cgtcgtcgat
      721 ctgggtaccg atcgcgggcc gaccacggtc gtcaccgcgg gcggcgccga tggccatccg
      781 acgcccacgt cccacaacgg ggatccaacg gcctatgtcg gccacgtgag catctatgtc
      841 ccgggagacc cggctcaccc gattaccgac gacttcctcg cccgtgactc agcgggaaag
      901 gcgaccggcg tgctgaacat ctcgcagatg aacccctatc agcggcaggc ctacctcaac
      961 tggctcctcg accctgctgt ccagaacttc ctcggcaccc gaatcgacgc gctgaaggtg
     1021 gccgccgggt tggcagccgg gaggtaacgc gcacgccgtc acgatcccca ggtgatcctg
     1081 gtcgtccagg tgtccacgac gtgcgtcgtg gtggcggtga cgatgcgatc cccgacgact
     1141 gccagggtct ccgtgtcagt ggccagcacc tggctggtcc cgtccaccac gtcgacgcgc
     1201 agccaggtcc gtgccgcgga cacgctccgg cactcgccgt tgtccctcgg cgcgggagca
     1261 ccgagcagga cgacccgatc ggatagccgg accggcgttg cgtcgtaatc cgagggacaa
     1321 agccctggaa tggggacgcg acgcaccgtc ccggcctcga gcacggccaa ggacagtcgt
     1381 gagtcccgtt tgatcgtgcc cgagacggtc ccgacgacga acgcgatccg atcaggggcg
     1441 agtgccgcaa cccttcggac gacaccgaga tcgacggagc ctgcaggcac ggacgtcccc
     1501 tctgggacgc cggacaaggg gtctggcggg ttggtccgcc acctcccggc aacctcgtcg
     1561 tggccggacg cagtgacccg attgatgagg acgtcctggt cagggttcaa tgtccgcccg
     1621 ccggtgtacg tcgaggccag cagcgtcgtc ccgtcgagaa tcgctagtcc gtcctctgac
     1681 accaggcgat ctggaaccac ccggccggtc ggaagggtga tcgagcctcg gcccagctcc
     1741 gagaccgcag cggacgctgg atggtcgacc cccaggaagg tggtcacttg ctgattggac
     1801 cggatcaacc agacctgtcc ggatgggtcg accgcgatct gttgcggagt ctcgctgccc
     1861 ggttgctggt catccttcgg caaccgggtg ggggtagggg tgccttgagg tccagcgatg
     1921 accactgaac ggtcgcccca cacggcatac agggtggaac cggacccgac gagcaacagc
     1981 cgcaacgcgt cggacgtctc ctgtggccag gtgatggtcg tctgcggcgg tccgctggga
     2041 gtcgtcgtgc atcccgaaac gccgaccacg acggtcgcca gcgcgacgag gacccccctg
     2101 aagatccttg gcatgacgcc caccctaacc gacggacctg ctggcgcagt cagcgtccga
     2161 ggttggcggg accgaacgcg tcgggaagca attcggacat cggcctcggc ccggtcgacg
     2221 tcgcgacgag gcagtcgggc ccgccgtgtt cccagagcag ttgtcggcag cggccgcacg
     2281 gcatgagcgt gtcgccttcg ggcccgacgc acgacacggc caccaaccga ccgccgccgc
     2341 cagccaccag ggccgacacc atcccgcact cggcgcacag ccccacgccg tagctcgcgt
     2401 tctccacatt gcagcccacg acaacccggc cgtcgtcgac caggcccgcc acgccgacgg
     2461 ggaaccccga atacggcgcg tatgccgtgt cgcgcacctc gatggccgct cgttgcagcg
     2521 cgacccagtc cggcgacggc atacccgagg ggtatgccgc gtccgcgcgg tcaggcgggg
     2581 gcggctgggt caatggcctt cgcccttgcg atagaccatg ccgtcggcct tgggcatgcg
     2641 taggttctgc gaggagaccg ataggacgac caaggtgacg atgtacggcg tcagggtggt
     2701 gacctcgttc ggaagggaat ccaggccgag gtagcccagc aggaaggtca cgccgatgcc
     2761 gatgaagatc gcaccgggca gtcggcggcc acggcgcaac cacaggacac ccaacgcgat
     2821 gacgacgatg ccggcgaaga tgaagagcgc gaggacggcg agggagttct gcgaacgaag
     2881 gcggacggca tcggcatacc cgaagagggt ggcgcctgcg gcgagcccgc cgggccgcca
     2941 gttgccgaag atcatcgccg cgaggccgat gtaaccgcga ccgttggtca tgccctcgcg
     3001 gtaactgccc gtgaacatga caaggaagag gccaccgagg cccgcgagcg cgccgcccca
     3061 gacaacggcg aggtacttga cccggacgac gttgacaccg agggactccg cagcccacgg
     3121 gttctcacct gcggagcgca gccgcagacc cagcgcggtc cgccagagca ggtatgccgt
     3181 gagcgggaac atcgccaggc cgaggagctc gtagggggtg atgccgacca tcagcccgcg
     3241 caggacgccc gcggcatcgg cgagcacggg tatgcccgtc cgttccacac tgcccagcac
     3301 gtcgggcccg gatgcgagga cggggatgtc gaaggtcgga atgttgccgg acatgggggg
     3361 cgactgggtc ggcccaccgc ccgtgcttgg ggtgaaggtg agctcggaga ggaagcgaac
     3421 cgtgcctgcg gcaagaaggt tgatggcgac accgctgacg atgtggtcga caccgaaggt
     3481 gacggtcgca agggcatgca gcaggccgcc gagggcacca ccgatgagag cggccacgag
     3541 cgcggccagt ggtccccagc gccacccggc gaagccggcc ccccagacac cgagcatcat
     3601 catgccctcg aggccgatgt tgacgacgcc ggcccgctcg ctgatcaggc cgccgaggcc
     3661 ggccagggcg acgggaaggg tgagccacac catcgcattg atggtgcccg acgcggtgag
     3721 gacgccctca cccgagatga cccgggtgat cgagagggcg accaatccga ggccggcgag
     3781 gagcagcagg cggttgcgtc gagcgcgccg ggcgacggtc gtggcagtgg gcgctgatgc
     3841 ggtggaagtg ctcatgccga cgctcctgcg gttgccggtc cgggggtgcc gctcaccgag
     3901 gtggtggggg c
//
LOCUS       DAOMZY010000322         3911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_22041_length_3911_cov_5.530861, whole genome shotgun sequence.
ACCESSION   DAOMZY010000322 DAOMZY010000000
VERSION     DAOMZY010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22041_length_3911_cov_5.530861"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PLK64_14555"
     CDS             complement(<1..438)
                     /locus_tag="PLK64_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPV81013.1"
                     /translation="MSTHVEVRKTYKLYIGGNFPRSESGRSYAVHAAPSRSGRPGAFL
                     ANAAAGSRKDARDAVVAARGAFPKWAGATAYNRGQVLYRVAEVMQGRAAQFEQEVAAA
                     EGLTAARARATVEAAIDRWVWYAGWSDKLAQVLGGLNPVAGPYF"
     gene            complement(435..1880)
                     /locus_tag="PLK64_14560"
     CDS             complement(435..1880)
                     /locus_tag="PLK64_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPV81014.1"
                     /translation="MATFDYAPAPESRAVVDLQPSYGLFIGGEFVDGSGEAFKTINPA
                     TEETLAEVAEASADDVDRAVKAARAAYRGVWGRMSGADRGKYLFRIARIMQERSRELA
                     VLETLDNGKPIKESRDVDVPLAAAHFFYHAGWADKLGYAALGPDPRPLGVVGQVIPWN
                     FPLLMLAWKIAPALACGNTVVLKPAETTPLTALLFAQICQQADLPPGVVNIVTGAGAT
                     GQAIVDHPGIDKIAFTGSTRVGKMIAREIAGTRKRATLELGGKAANIVFDDAPLDQTI
                     EGIVNGIFFNQGQVCCAGSRLLVQESIADDVERRLKRRLATLRVGDPMDKNTDVGAIN
                     SRRQLDRITELSAAGEAEGAARWDTDCPLPKNGFWFRPTVFTGVSQSHRIAQEEIFGP
                     VLSVLTFRTPAEAVAKANNTPYGLSAGVWTEKGSRILWMADQLRAGVVWANTFNQFDP
                     TSPFGGYKESGYGREGGRHGLEAYVRTGGAA"
     gene            complement(1896..2885)
                     /gene="deoC"
                     /locus_tag="PLK64_14565"
     CDS             complement(1896..2885)
                     /gene="deoC"
                     /locus_tag="PLK64_14565"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPV81015.1"
                     /translation="MPDPLARARDLLGVSRLSDTALTRWLHALPGVDQVGAEARAASL
                     GTRSIKAASKAWAIDLAISMIDLTTLEGADTPGKVRGLAARALNPDPADPSTPSPAAV
                     CVYGDMVPFAREVLQGSTVRIAAVATAFPSGRASMPVKLADTRDVVANGADEIDMVID
                     RGAFLAGHYRQVFDQIVAVKEACRRPDGTSARLKVIMETGELVTYDNVRRASWLSMLA
                     GGDFIKTSTGKISPAATLPVTLIMLEAVRDWREATGQQLGVKPAGGIRTSKDAIKFLV
                     AVNEVAGPDWLDNHWFRFGASSLLGDLILQRQRLAIGAYSGADYVPDDSPGIY"
     gene            2994..3692
                     /locus_tag="PLK64_14570"
     CDS             2994..3692
                     /locus_tag="PLK64_14570"
                     /inference="COORDINATES: protein motif:HMM:NF022216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPV81016.1"
                     /translation="MSASTPASGGPPHESGGPGGSDSGHLVHVLRPRGSVFLGIVAIV
                     AASVMLAFSGGAGLGFVGAMALLATWSFVLLVRPHVRVSIEGVAVHNPFRRTFVPWRV
                     LEDAGSRWNLELYAAERTIRVWAISNPMIRPRVGMSLGFGGIGAPRAPGDAAASADPF
                     AKAKAEGALRHGATSAPAAARLIEAAREDWLDAVDEGIVAEPPNPRIVARWDLWDIAL
                     VVVPAIFILVAVLT"
BASE COUNT          529 a         1268 c         1464 g          650 t
ORIGIN      
        1 gaagtagggc ccggcgaccg ggttgagccc gccgaggacc tgggcgagtt tgtcggacca
       61 cccggcatac caaacccagc ggtcgatggc ggcctcgacg gtggcgcgtg ctcgggcggc
      121 cgtgaggccc tcggcggcgg cgacctcctg ctcgaactgc gcggcgcgcc cctgcatcac
      181 ctcggcgacc cgatagagca cctgacccct gttgtatgcc gtcgcgccgg cccacttggg
      241 gaaggcgccc cgggccgcga cgacggcatc acgggcgtcc ttgcgcgagc ccgcggcggc
      301 gttggcgagg aaggccccgg gacggccgct gcggctggga gccgcgtgga cggcatacga
      361 ccgaccgctc tcactgcgcg ggaaattgcc gccgatgtag agcttgtagg tcttgcggac
      421 ctcgacgtgc gtgctcacgc cgcacccccc gtccggacgt aggcttccag gccgtggcgg
      481 ccgccctcgc ggccgtaacc ggactctttg taaccgccga acggcgaggt cgggtcgaac
      541 tggttgaacg tgttggccca gacgacgccg gcccggagtt ggtcggccat ccagaggatc
      601 cgcgagccct tctcggtcca gacgcccgcg gacaggccgt agggcgtgtt gttggccttg
      661 gcgaccgcct cggccggggt gcggaaggtg agcaccgaca ggaccggccc gaagatctcc
      721 tcctgggcga tccggtggct ctggctgacc ccggtgaaga cggtcgggcg gaaccagaaa
      781 ccgttcttgg gcagcgggca gtcggtgtcc caccgggccg cgccctcggc ctcgccggcg
      841 gcgctcagct cggtgatccg gtcgagttgg cggcggctgt tgatggcgcc gacgtcggtg
      901 ttcttgtcca tcgggtcacc gacccgcagc gtggccaggc ggcgcttgag tcggcgttcg
      961 acgtcgtcag cgatcgactc ctggacgagc aatcggcttc cggcgcaaca gacttggccc
     1021 tggttgaaga agatcccgtt gacgatgccc tcgatggtct gatccagcgg tgcgtcgtcg
     1081 aagacgatgt tggcggcctt gccgccgagc tcgagggtgg cgcgcttgcg ggtgccggcg
     1141 atctcgcggg cgatcatctt gccgaccctc gtcgagccgg tgaaggcgat cttgtcgatg
     1201 cccgggtggt cgacgatcgc ctggccggtt gcgccggcgc cggtgacgat gttgacgacg
     1261 cccggcggca gatcggcttg ctggcagatc tgcgcgaaga gcagcgcggt caggggagtg
     1321 gtctcggcgg gcttgaggac gacggtgttg ccgcaggcca gagccggggc gatcttccac
     1381 gcgagcatga gcagtgggaa gttccacggg atgacctggc cgacgacgcc cagcgggcgc
     1441 ggatctgggc ccagggcggc atacccgagt ttgtcggccc accccgcgtg gtagaagaag
     1501 tgcgccgccg ccagcggcac gtcgacatcg cggctctcct tgatcggctt gccgttgtcg
     1561 agggtttcga ggacggccag ctcgcgggag cgctcctgca tgatgcgggc gatccggaag
     1621 aggtacttcc cgcggtcggc gccagacatg cgaccccaca cgccgcggta tgccgcccgc
     1681 gcggctttga cggcgcggtc cacgtcgtcg gcgctcgcct ccgcgacctc ggcgagggtc
     1741 tcctcggtgg ccgggttgat ggtcttgaac gcctcgccag agccgtcgac gaactcgccg
     1801 ccgatgaaca ggccatagga gggctgcagg tcgacgacgg cccgtgactc gggggccggt
     1861 gcgtagtcga aggtcgccat gtcaggtcct cagagtcagt agatgccggg ggagtcgtcc
     1921 gggacgtagt cggccccgga gtaggcgccg atggccagcc gttgccgctg caggatgagg
     1981 tcgcccaaca ggctggacgc tccgaagcgg aaccagtggt tgtccagcca gtcgggcccg
     2041 gcgacctcgt tgacggccac gaggaacttg atcgcgtcct tgctggtgcg gatgcccccg
     2101 gcgggcttca cgccgagttg ttggccggtg gcctcccgcc agtcgcggac ggcctcaagc
     2161 atgatgaggg tgacggggag ggtcgcggcg ggggagatct tgccggtgct cgtcttgatg
     2221 aagtcgcctc cggcgagcat cgacagccat gaggcccggc ggacgttgtc ataggtgacc
     2281 agttcgccgg tctccatgat gaccttgagc cgggcgctcg ttccgtcggg tcggcggcag
     2341 gcctccttga ccgcgacgat ctggtcgaac acctgccgat agtgcccggc gaggaaggca
     2401 ccgcggtcga tgaccatgtc aatctcgtcg gcaccgttgg cgaccacgtc gcgcgtgtcg
     2461 gcgagcttga ccggcatact tgcgcggcct gaagggaacg cggtggcgac ggcggcgatg
     2521 cgaactgtgc tgccctgcaa cacttcccgc gcaaagggga ccatgtcgcc gtagacgcac
     2581 accgcagccg ggctgggggt gctcgggtcg gcggggtcgg ggttgagcgc gcgggcagcc
     2641 aagccgcgga ccttgccggg ggtgtcggcg ccttcgagcg tggtgaggtc gatcatcgag
     2701 atcgccaggt cgatcgccca ggccttggat gccgccttga tcgagcgtgt ccccagcgag
     2761 gccgcgcggg cctcggcccc gacctggtcg accccgggga gggcgtgcag ccagcgggtg
     2821 agggcggtgt cggagaggcg ggagacgccg agcaggtccc gcgcccgggc caaaggatcc
     2881 gggatcgcgt cgacggcggg gtcgaggcga gtgccaacaa cggtgtcggg gacgctcacc
     2941 gaagcgagtc taggcccacc gtcaacggcg cgcgccgtgt catgatggcg cggatgagcg
     3001 cctccactcc tgcctccggc ggcccgcccc acgagtctgg cggtcccggc ggttctgact
     3061 ccggccacct ggttcacgtc ctgcggccac ggggttctgt cttcctcggg atcgtggcca
     3121 tcgtcgctgc ctcggtcatg ctggccttct ccggcggcgc gggactgggc ttcgtcggtg
     3181 cgatggcact gctggcgacg tggtcgttcg tcttgctcgt gcggccgcac gtccgcgtgt
     3241 cgatcgaggg ggttgcggtc cacaacccgt tccggcgcac cttcgtcccg tggcgagtgt
     3301 tggaggacgc cgggtcgcgc tggaaccttg agttgtatgc cgcggagcgc accatccgcg
     3361 tctgggcgat ctccaacccg atgatccgcc cgcgagtggg catgtcgctg gggttcggcg
     3421 ggatcggggc cccgagggcg ccgggggatg ccgcggcgtc tgcggacccg ttcgcgaagg
     3481 cgaaggccga aggagcgctg cgacatgggg cgacctccgc gccggccgcg gcccgcctca
     3541 tcgaggcggc gcgtgaggat tggttggacg cggtcgacga ggggatcgtt gccgagccgc
     3601 cgaatcctcg gatcgtcgct cgctgggacc tgtgggacat cgccctggtg gtcgtccccg
     3661 cgatcttcat cctcgtcgct gtcctgacct gatagacccc cgtggttgcg agaccaacga
     3721 gaggtgggcg gtggccatcg atggctgagt tggcgggggc gatcatcgac cttgttcttt
     3781 ccgtcctcac ggcccgggcg atgtcgtggc gcccgttcga cggcgttcgc gcacggcgaa
     3841 ggacgacgga attcgcacgt ggtcagcagg ttgccgtccc gttccgctgg gaggacgttc
     3901 ctctgctgct g
//
LOCUS       DAOMZY010000323         3900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_22130_length_3900_cov_5.985176, whole genome shotgun sequence.
ACCESSION   DAOMZY010000323 DAOMZY010000000
VERSION     DAOMZY010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22130_length_3900_cov_5.985176"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PLK64_14575"
     CDS             complement(<1..215)
                     /locus_tag="PLK64_14575"
                     /inference="COORDINATES: protein motif:HMM:NF013166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HPV81017.1"
                     /translation="MTDRLKLFCFPFAGATAAVYHRFRRYLPPRIDVVPVEMAGHGSR
                     MKEPLIDSVSGHVDDLLPRLTDQMAAGP"
     gene            complement(226..>3900)
                     /locus_tag="PLK64_14580"
     CDS             complement(226..>3900)
                     /locus_tag="PLK64_14580"
                     /inference="COORDINATES: protein motif:HMM:NF026115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPV81018.1"
                     /translation="ATGTVLGDPIEVDGLTTAFARHTSRKQFCALGSVKPNLGHGVSA
                     ASMSSLLKVLLAMRARKLPPNINFEEPNPYIAFHESPLYVSDVLQPWTPNGPRLLAAV
                     SSFGFGRTNAHVVLEEAPAREVPADPAPAPDPDRLVFALSARTEAALWHYVRAVHDHL
                     VDHPQLSPADVCFTLAVGRMQFEHRLAFEFAGRAELLARLAELSSPDRHLAGVHVGRH
                     RVISANMIAQDAHDLTGTQQRALTAQAAALLTGDPLGAEARSDLCDLFVGGASVDWAA
                     TFSTPGLGRVPLPTYPFQRKHFWIEPEPESPTLVEPDALHPLVEKLLARSRSEDIYGT
                     RFEVGKQWILTEHVINGHNVIPGTAFLEMMHAACAQTCGQPQLELADVVFHASATVRP
                     DEPLDVQITVRTVEGAFEIEILSEDPDLGWVTHATGRGIPLAATRPPTVLDPAITRAR
                     CDEVLPFDLSKEYGGFRFGPRWRNVTEIVYNSRQEIFTDLVMAEDFLADLDEYFLHPA
                     LLDNAINIGSEFLIQNVTHTMFLPFACKSIKIYAPLPQSFVSVQVIRNPIKNDMGAAS
                     IDVTLVDAAGRVILTLEKYSLKRVNHELFVNSVYHRVGFKETPLPPTAVGGSAHSLAA
                     STVLLVADQPDQGLVIAEASRQLEAVGAKVVVAATDADLDLVFAALGDDRLGAVVHAG
                     AFGRRSPRSAQDLTSQVDAGTHSLVRLAQALARRESAGQLPLLVLVDAVSPVGGNADV
                     QNPVGACVGALAKVLSAEHARLTVGCLDTQAEVVPSLVDEIAALQDGALVVWRGAHRY
                     AAVVEEAQLETFPAEPVEIRDTGVYLITGGLDGIAAAIIEGLTTEERHPRFAVLSRTP
                     IPDDPAEWDAAANAGPPAVAARVARLREMRERGIDVHPVAGDVADLDGMRQVCAQLRD
                     RFGAINGVVHGAGLPAGSYVVNTSLEEFDRVLRPKVAGTWVLEEVTREDDLAFFLLSS
                     SLISVFGPAGQGAYAAANAYLNATASMRRARGQKSTAIAWSVWSESGMATAYDADQVR
                     GVFHSLTDAAGAEAAVGVLDRDVAVLLVGGLDHGRIREAGGAIGLPLSDALRAKLRMG
                     QPRKQERPRRREVVVLSSEPLTDTEVAVANIWGTLQALDEVSVHEPFSDSGGDSLVIS
                     QFVNALNEHQQTVLDISDVYANPTVRQLAGYLDGRQAPPLPEAAPTAERVLTVEEIMD
                     GLDRGELSMDEAQQLLERVE"
BASE COUNT          637 a         1279 c         1391 g          593 t
ORIGIN      
        1 ggtccggccg ccatctggtc ggtcagccgg ggcagcagat catcgacatg acccgacacc
       61 gagtcgatga gaggctcctt catccggctc ccgtgtccgg ccatctcgac ggggacgaca
      121 tcgatccgcg ggggcagata ccgccggaac cggtggtaga cggccgccgt ggcgccggcg
      181 aaggggaagc agaacaactt caggcgatca gtcatcgggc cgacctcact ccactcgttc
      241 cagcagctgc tgagcctcat ccatgctgag ctcgccgcga tcgagcccgt ccatgatctc
      301 ttcgacggtg agcacccgct cggcggtcgg cgctgcctcc ggcagtggcg gcgcctgccg
      361 gccgtcgagg tagccggcca actggcgcac cgtcgggttc gcatagacgt ccgagatgtc
      421 cagcacggtc tgctggtgtt cgttgagcgc gttgacgaac tgcgagatga ccaacgagtc
      481 acccccggag tcgctgaacg gctcgtggac actcacttcg tcgagggcct gcagggtgcc
      541 ccagatgttc gcgacggcaa cctcggtgtc ggtcaacggt tcggagctga ggacgaccac
      601 ctcgcgacgg cgagggcgtt cctgctttcg cggctggccc atgcgcagct tggcccgcaa
      661 ggcgtcagac agcggcaggc cgatggctcc tccggcctca cggatccggc cgtggtccaa
      721 accgcccacg agcaggacgg caacgtcacg atcgagaacg cccaccgcgg cctcggcccc
      781 ggcggcgtcg gtcaggctat ggaagacgcc gcgcacctga tccgcgtcgt atgccgtcgc
      841 catgccgctt tcgctccaca ccgaccaggc aatcgctgtg gacttctgcc cacgagcccg
      901 acgcatcgac gccgtcgcgt tgagataggc gttggcggcg gcatacgccc cctggccggc
      961 gggaccgaag acggagatga gggacgagga gagcaggaag aaggcgaggt cgtcctcccg
     1021 ggtgacctcc tccagcaccc acgtgcccgc aaccttggga cgcagcaccc gatcgaactc
     1081 ttcgagcgag gtgttgacga cgtaggagcc ggcgggcagc ccggcgccgt gaaccacgcc
     1141 gttgatcgcc ccgaaccggt cccgaagttg cgcgcacacc tgcctcatcc cgtcgaggtc
     1201 agccacgtca cccgcgaccg ggtggacgtc gatccctcgc tcgcgcatct cgcgcaaccg
     1261 ggcaacgcgg gccgccactg ccggggggcc ggcgttggcc gccgcgtccc actcggcggg
     1321 gtcgtccggg atcggggtcc ggctgaggac ggcgaacctg gggtgacgct cctccgtcgt
     1381 gaggccctcg atgatcgctg cggcgatccc gtcgagcccg cccgtgatga ggtagacgcc
     1441 agtgtcccgg atctccaccg gctcggccgg gaaggtctcc agttgcgcct cctcgacgac
     1501 cgcggcatac cgatgagcgc ctcgccagac cacgagggcg ccgtcctgca gcgctgcgat
     1561 ctcatcgacg agagatggca cgacctcggc ttgcgtatcc aggcacccca ccgtgagtcg
     1621 cgcgtgctcg gcggacaaca ccttggcgag cgcacccacg caggcgccga cggggttctg
     1681 cacgtcggcg ttcccaccga ccggcgagac cgcgtcgacg agcacgagca gcggcaactg
     1741 accggccgac tcgcgtcggg cgagcgcctg cgccaggcgg accaggctgt gcgttccggc
     1801 gtcgacctgg ctggtgagat cctgcgccga ccggggtgac cgtcggccga aagcgccggc
     1861 gtggacgacg gcgcccaacc ggtcatcgcc gagtgccgcg aacaccaggt cgaggtcggc
     1921 atcggtggcc gcgacgacga ccttcgcgcc gacggcctcc aactggcggc tggcctccgc
     1981 gatgaccagc ccctggtcgg gttggtcggc cacgagcaag acggtggagg cggccagcga
     2041 gtgggcgctg ccgccgacag cggtcggagg cagcggggtc tccttgaacc cgacgcggtg
     2101 atagacgctg ttgacgaaca gttcgtggtt gacccgtttg agggagtact tctcgagggt
     2161 gaggatcacc cggcccgccg catcgacgag ggtgacgtcg atcgacgcag cacccatgtc
     2221 gttcttgatg gggttgcgga tcacctggac gctgacgaac gactgaggca acggggcgta
     2281 gatcttgatg ctcttgcagg cgaacggcag gaacatcgtg tgggtgacgt tctggatgag
     2341 gaactccgac ccgatgttga tggcgttgtc caacagcgcg gggtgcagga agtactcgtc
     2401 gagatcggcg aggaaatcct cggccatgac caggtcggtg aagatctcct gccgggagtt
     2461 gtagacgatc tccgtgacgt tgcgccaccg tggcccgaac cggaagccgc cgtattcctt
     2521 ggagaggtcg aacggcagca cctcgtcgca ccgagcgcgc gtgatcgccg ggtccaggac
     2581 ggtcggtggc cgggttgcag ccagaggtat gccgcgtccg gtcgcgtgag tgacccagcc
     2641 aaggtcgggg tcctcactga ggatctcgat ctcgaaggca ccctcgacgg tgcgcaccgt
     2701 gatctgcacg tccagcggct cgtccggacg cactgtcgcc gaggcatgga agaccacgtc
     2761 cgccagctca agctggggct gcccgcacgt ctgcgcgcag gcggcatgca tcatctcgag
     2821 gaaggccgtt ccgggaatga cgttgtggcc gttgatgacg tgttcggtga ggatccactg
     2881 cttcccgacc tcgaaccgcg tgccgtagat gtcctctgag cgagatcggg cgagcagctt
     2941 ttcgacgagt gggtgcagcg cgtcgggctc gaccagcgtg ggtgactccg gctccggctc
     3001 gatccagaag tgcttgcgct ggaaggggta ggtggggagc ggcacgcgcc ccagccccgg
     3061 tgtcgagaac gtggccgccc agtcgacaga ggcaccgccg acgaagaggt cgcacaggtc
     3121 cgagcgcgcc tcggctccga gcggatcacc cgtgagcagc gcggcggcct gcgccgtgag
     3181 ggcgcgctgt tgggtgccgg tgaggtcgtg cgcgtcctgc gcgatcatgt tggccgagat
     3241 cacccggtgc cggccgacat ggacgcccgc caggtggcgg tcaggactgg acaactccgc
     3301 cagccgggcc aggagttcgg cacggccggc gaactcgaag gccagccggt gctcgaactg
     3361 catccggccg acagcgaggg tgaagcagac gtcggccggg ctcagctgcg ggtggtcgac
     3421 aagatggtcg tgcacggcgc gcacatagtg ccacaaggct gcttcggtgc gcgccgagag
     3481 tgcgaagacg aggcggtcgg gatcgggggc cggggcggga tcggccggga cctcgcgggc
     3541 gggcgcctcc tcgaggacca catgggcgtt cgtccgcccg aacccgaacg agctcactgc
     3601 ggccagcagc cggggaccgt tcggggtcca cggttgcagg acatcgctga cgtacaacgg
     3661 tgattcgtgg aaggcgatgt aggggttggg ctcctcgaag ttgatgttgg gcgggagctt
     3721 ccgggcccgc atggcgagga gcaccttgag cagtgaggac atcgaggctg cgctcacgcc
     3781 gtgccccaga ttgggcttga ccgagcccag ggcgcagaac tgcttgcgcg aggtatgccg
     3841 tgcgaaggcg gtcgtcagac cgtcgacctc gatggggtca ccgagcacgg ttcccgtggc
//
LOCUS       DAOMZY010000324         3895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_22172_length_3895_cov_5.239583, whole genome shotgun sequence.
ACCESSION   DAOMZY010000324 DAOMZY010000000
VERSION     DAOMZY010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22172_length_3895_cov_5.239583"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLK64_14585"
     CDS             complement(<1..485)
                     /locus_tag="PLK64_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81019.1"
                     /translation="MRWLAVGEAAIPEGTGWLSEQEAARAATMRFTKRRTEYRLRRLA
                     GKRAVASVLGRPVNPTSLAGIEVLNRRGGAPYVVVDGEHAQVDVSLTDRAGWAVCLVG
                     PEGELTGGTLGVDLEAVEPRSRGFVTDYLTAYEQEYVRSQGELGSEGWDVAANLLWSA
                     K"
     gene            complement(638..710)
                     /locus_tag="PLK64_14590"
     tRNA            complement(638..710)
                     /locus_tag="PLK64_14590"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(675..677),aa:Asn,seq:gtt)
     gene            complement(792..1145)
                     /locus_tag="PLK64_14595"
     CDS             complement(792..1145)
                     /locus_tag="PLK64_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81020.1"
                     /translation="MSKIAFPTTPARRWATAAAAAAVVGAGAVGIHLADAGAATIAAT
                     TSTVPDGTRIQQLPANGDAGGAAAGQVNGAPPPNGQAGTKPGARGAQTGSGGFQQAQA
                     PGRGSGGGQAMTQGS"
     gene            complement(1213..2208)
                     /locus_tag="PLK64_14600"
     CDS             complement(1213..2208)
                     /locus_tag="PLK64_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPV81021.1"
                     /translation="MDSQTVTRPVPVVQAEFRAIGTTNRILATDLTVLTEATAIAREH
                     LDALDLAVSRFRPDSEVCRLAARAAGGPASTLASPTFAAALRAALHAARVTDGLVDPT
                     VGSAVVANGYDADLDAVRDRASFQRTRLADVPGWENVEIDASDRVTVPAGCLIDLGAT
                     AKAFAADTIAGLLADRLPGGFLVNLGGDIAVSGPLPAGGWRVGVQTADGAVAQVVTTT
                     GQGLATSSTRLRTWATDPGHNRAHHIVDPRTGLTAPSTWAQVSCAGASALEANAASTA
                     AIVLGPDAPAWLQAHGIPARLDGCDGEVTLTCGWPVPDSDASQARAEIANALASR"
     gene            complement(2222..2473)
                     /locus_tag="PLK64_14605"
     CDS             complement(2222..2473)
                     /locus_tag="PLK64_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81022.1"
                     /translation="MSTPRSTSPGTVRRWSAAALAAVAVTAGGFGIQHAAAQTSTVTA
                     SATTTSSTTTNSSSTSSGLTAVAAPASSSGSAQSTTSGS"
     gene            complement(2638..3168)
                     /locus_tag="PLK64_14610"
     CDS             complement(2638..3168)
                     /locus_tag="PLK64_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81023.1"
                     /translation="MRLPGWRRTDPDHTPPPEVLAVLAESVGPDRVLASARSDQTWLL
                     ATTHRFAAVFADGTVRWLRPWHEVDAASWGRESETLTVTFVDGGRPTMLPMASARTFL
                     MTLRERVQASVVASVDLPLEGARKARAVIRQNLATGELIEQLVLGRGTRPSESIDAAA
                     AIAFAELRDDTGLPPR"
     gene            complement(3183..>3895)
                     /locus_tag="PLK64_14615"
     CDS             complement(3183..>3895)
                     /locus_tag="PLK64_14615"
                     /inference="COORDINATES: protein motif:HMM:NF019883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPV81024.1"
                     /translation="EAKGRAGSAAAGRAAGERNSGDRGSGASGSGASGSGGGGSADPR
                     HEGGDRHEVGEESLEPSDAELAASLPLPNLRDPVVQAERQLLQCLLQFPDVVDPLSLP
                     LLEVGDFSAPAHQVVFSTVAGVGLPAPTMSAPAWHSALAEAAPLAIHPMLAELAVAPL
                     PVLLDPTTGRPAARYVDSLVTRVREVALSRQIADTLGQLRRQDASPDSDPDRRRATAI
                     RLQDLQRALAALRDRDDE"
BASE COUNT          581 a         1375 c         1378 g          561 t
ORIGIN      
        1 tccttcgccg accacagcag gttggcggcc acgtcccagc cctcgctgcc gagttcgccc
       61 tgggagcgca catactcctg ctcgtatgcc gtgaggtagt cggtcacgaa cccgcggctg
      121 cgcggctcca cggcctccag atcgaccccc agcgtgccgc ccgtcaattc gccctcgggc
      181 ccgaccagac acaccgccca gcccgcccgg tcggtcaatg agacatccac ctgagcgtgc
      241 tcgccgtcaa cgacgacgta tggcgctccc ccacgccgat tgagcacctc gatcccggcc
      301 aggctggtgg ggttcaccgg acggcccagg acggatgcga ccgcacgctt gccggccagt
      361 cgccgcagcc gatactcggt gcgccgcttc gtgaagcgca tcgtcgccgc gcgggcggcc
      421 tcctgctcgg agagccagcc cgtcccctcg gggatcgcgg cctcaccgac cgccagccat
      481 cgcatgccgc gatcatcccc cacactcggg atgccaggcg agccgacgga cgcatcggag
      541 gcggcggaac ggcataccac cgcacgaacc ggacgccacg aagaggtgtg ccggagacga
      601 gtgcgggacc ggccccgaag gaccggtccc gcgtctgctc ccccacttgg actcgaacca
      661 agaacctgcc ggttaacagc cggctgctct gccaattgag ctatggagga atggtgcccg
      721 gttaggttag caaaccgagg ccccacaggc taaacggcat cccgtatgcc gcgacgcctg
      781 gcctcccgcg ctcaggaacc ttgggtcatg gcctgtccgc cgccactccc tcgaccgggc
      841 gcctgtgcct gctggaagcc tccggagccg gtctgtgcgc cgcgagcacc gggcttcgtc
      901 cctgcttggc cattcggcgg cggagccccg ttcacctgac cggctgcagc gccgcccgcg
      961 tcgccgttgg ccgggagttg ctggatccgc gtgccgtccg gcactgtcga tgtcgtcgcc
     1021 gcgatcgttg ccgccccggc gtcggcgagg tggattccga ccgcgccggc tccgacgacg
     1081 gccgccgctg ctgctgccgt cgcccaacgt cgggcaggtg tcgtggggaa ggcgatcttg
     1141 ctcatggtgc gctccttcgg aaggggttgg atcgttgacc tgagcgtcgc ccggccggat
     1201 gtgccagacc tgtcagcggg atgcgagcgc gttggcaatc tcggcccgcg cctgtgaagc
     1261 gtcactgtcc gggaccggcc aaccacacgt gagcgtgacc tcgccgtcgc agccgtccag
     1321 gcgagccggt atgccgtgcg cctgcagcca ggcgggcgcg tccgggccga ggacgatggc
     1381 tgccgtcgaa gcggcgttgg cctccagggc ggaagcaccg gcacagctca cctgggccca
     1441 cgtcgacggc gccgtgaggc cggtcctcgg gtcgacgatg tggtgggcgc ggttgtggcc
     1501 agggtcagtg gcccaggtgc gcagtcgcgt cgatgaggtc gccaggccct ggccggtcgt
     1561 cgtcacgacc tgggccacgg caccgtcggc ggtctgcaca cccaccctcc agccgcctgc
     1621 ggggagtggc ccgctgaccg cgatgtcgcc gccgaggttg acgaggaatc cccccggcag
     1681 ccggtcagcc aacagccccg cgatggtgtc ggcggcgaac gccttggccg tcgcgcccag
     1741 gtcaatgagg caaccggcag gcacggtgac ccggtccgac gcatcgatct cgacgttctc
     1801 ccaccccggc acgtccgcca accgggtgcg ctgaaaggaa gcccggtcgc gaacggcgtc
     1861 caggtcggcg tcatacccgt tggccaccac cgccgatccc acggtgggat cgacgagccc
     1921 atcggtgacc cgtgcggcat gcagagcggc ccggagggca gcggcgaagg tcggagaagc
     1981 cagcgtcgac gctggcccac ctgcggcgcg agcggcgagg cggcatacct ccgagtcggg
     2041 tcggaaccgc gagactgcga ggtcgagcgc gtcgaggtgc tcgcgcgcga tcgcggtcgc
     2101 ctcagtaagg accgtgaggt cggtcgcaag gatccggttc gtcgtgccga tcgcacggaa
     2161 ctcggcctgc acgaccggaa ctgggcgggt caccgtctgc gaatccatga tggccgaccg
     2221 ctcaggagcc cgaggtcgtg gactgtgcgg agccgctgct gctcgcggga gccgcgaccg
     2281 cggtgaggcc gctggaggtc gaggaggagt ttgtggtcgt tgaggacgtg gtcgtggccg
     2341 aggccgtcac ggtgctcgtc tgtgcggccg catgttggat gccgaacccg cctgccgtca
     2401 cggccaccgc ggccagcgca gcggcggacc aacgacggac ggtgcctggg gaggtggatc
     2461 gcggggtgct catcgtcggg cctttcattg gtaggtggtc ctaccgtccc cgagcacctt
     2521 gtgccgccgc tgtccggctc cggtggggcg ggcatgcatc cgctgtcggt tgcctgtgcc
     2581 cagcgtcgcc tatgtcgaag gggtatgccg cggcatcacg tccgcgtcgc tgccggctca
     2641 gcgagggggc aggccggtgt cgtcgcgaag ctcggcgaag gcgatcgcag ccgccgcgtc
     2701 gatgctctcc gacgggcggg tgccgcggcc aaggaccagt tgttcgatga gctcgccggt
     2761 ggcgaggttc tggcggatga ccgcacgcgc cttccgggca ccttcgaggg gcagatcgac
     2821 cgatgcgacg acgctggcct ggacccgctc gcgcagggtc atgaggaagg tgcgggcgct
     2881 ggccatcggc agcatggtcg ggcgaccgcc atcaacgaaa gtgactgtga gagtctccga
     2941 ttcgcgcccc cacgacgccg cgtcgacctc gtgccagggc cgcagccaac gcactgtgcc
     3001 atcggcgaac acggccgcaa agcgatgggt tgtggcgagc agccaggtct gatcggagcg
     3061 cgcactcgcc aggactcggt cgggtccgac tgactcggcg agcaccgcca gcacctcggg
     3121 aggcggcgtg tggtccgggt cggtccgtcg ccatccgggc agtcgcatag gcaccatcct
     3181 tctcactcgt cgtcgcggtc gcgcaaggca gcgagcgctc gttgcagatc ttgcaggcgg
     3241 atcgccgtgg ctcggcgccg gtcggggtcc gagtcgggcg aggcgtcctg tcggcgcaat
     3301 tggcccaggg tgtcggcgat ttggcgcgag agggccacct cgcggacccg cgtgaccaac
     3361 gagtcgacgt agcgagccgc cgggcgaccg gtggtcgggt cgagcaggac cggaagtgga
     3421 gccactgcca actcggcgag catcgggtgg atcgccaacg gagcggcctc ggcgagggca
     3481 ctgtgccacg cgggcgcgga catcgtgggc gcgggcaacc cgacaccggc gaccgttgag
     3541 aagaccacct gatgggccgg agccgagaag tccccgacct ccagcagagg cagcgagagc
     3601 gggtcgacca catccgggaa ttgcaggaga cactgcaaca actggcgctc cgcctggacg
     3661 accgggtcac gcagattcgg aagcggcagg gatgcggcaa gctcggcgtc gctcggctca
     3721 agggactcct caccgacctc gtgccggtca ccgccctcgt gcctcggatc agccgagcca
     3781 ccgccacccg aaccactcgc acccgaacca ctcgcacccg aaccgcgatc ccccgaattg
     3841 cgttctcccg cagcccgccc ggccgcagca gagccggcgc gccccttcgc ctcct
//
LOCUS       DAOMZY010000325         3879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_22304_length_3879_cov_6.136245, whole genome shotgun sequence.
ACCESSION   DAOMZY010000325 DAOMZY010000000
VERSION     DAOMZY010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22304_length_3879_cov_6.136245"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PLK64_14620"
     CDS             complement(<1..377)
                     /locus_tag="PLK64_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010156925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81025.1"
                     /translation="MPSALPDGDPAPATGRLPAGALDAIAATPFGVYVHVPFCRVRCG
                     YCDFNTYAIPELGAHGAAEVSDYADQVLRELDLAVDVLTDGGSGPGPRQVETVFVGGG
                     TPTLLPAADLAKVILGRGDEGRAA"
     gene            1011..1457
                     /locus_tag="PLK64_14625"
     CDS             1011..1457
                     /locus_tag="PLK64_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPV81026.1"
                     /translation="MVEMTRADAVREIAAGYALHRGPLLPVLHAVQDRFGAIERGDLV
                     AVADVLNLSIADVHGVMTFFHDFRAAPPPTHVVAICRGEACQSVGAERLMADVRADLQ
                     EASDVEVHEVFCLGNCALGPSGTVDGRLFGHLTASRVGASLPGRSS"
     gene            1454..3037
                     /locus_tag="PLK64_14630"
     CDS             1454..3037
                     /locus_tag="PLK64_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPV81027.1"
                     /translation="MTRFFVPGDAAAVSVGADEVAAALTGQPGVEVVRTGSRGMLWLE
                     PLVEVDTADGRVGFANVSPGQVGALLADATSVCSREPTASAGPAPEADHPAYLGPLDE
                     HPWMTRQARLTTARLGVVDPADPDDYARHGGWVGLRRALGMAPEAVVEEVVASGLRGR
                     GGAGFPAGVKWRTVAGGPGPLKFVTINADEGDSGTFADRMLMEGDPFALIEGMAITAW
                     AVGASEGYVFIRSEYPHAIATMRRAVETARTGGWLGPDILGSGLAFDVEVRVGAGAYI
                     CGEETSMLSTIEGRRGEVRAKPPIPALSGLFGRPTVVNNVLTVAAVPSILADGPEAYA
                     AHGVDRSRGTQVFQLAGNVARGGLVEVDFGITLRDLVEGFGGGTRSGRPVRAVQVGGP
                     LGAYVPADRLDVPLAYEAMADAGFMLGHGGVVVFDDTVDMGRMARFAMEFCAKESCGK
                     CTPCRIGSQRGVETIDRLVAGGTESDRRANLALLQDLCTTMERGSLCAMGGLTPMPVR
                     SALTYFPEDFGLSADGEPR"
     gene            3034..>3879
                     /locus_tag="PLK64_14635"
     CDS             3034..>3879
                     /locus_tag="PLK64_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPV81028.1"
                     /translation="MTLAPERDLGTAAVVGEPTATVTIDGIPVRVAPGTSVLRAASLA
                     GVEIPRLCATDRLEAFGSCRLCLVEVDGVRGTPASCTTLCSDGMSVATDTPMVRQLRH
                     GVMELYLSDHPADCAGCERGSCDLGRLAADAGVAEVRYGLGRSHADATTDVSNPYFAF
                     DPAACIVCSRCVRACGEVQGTFALSVAGRGFDSQISPSGTDFLASECVSCGACVQACP
                     TSALEEKSLVSLGMPSRAVVTTCAYCGVGCSFRAEVRDDPEGTTLVRMVPWKDGGANE
                     GHSCVK"
BASE COUNT          518 a         1337 c         1408 g          616 t
ORIGIN      
        1 gctgcgcgac cttcatcgcc gcggccgagg attaccttcg caaggtcggc ggccggcaag
       61 agggtgggcg tgccaccacc gacgaacacg gtctcgacct ggcgcgggcc tggcccgctg
      121 ccgccgtcgg tgaggacgtc gacggccagg tccaattccc gcagcacctg gtcggcatag
      181 tcgctgacct cggccgctcc gtgggcaccc aactcaggga tcgcgtaggt gttgaaatcg
      241 cagtagccac agcgcacgcg gcagaagggg acgtggacgt agacgccgaa cggcgtggcc
      301 gcgatcgcgt cgagagcgcc ggccgggagg cgacccgtag cgggggcggg gtccccgtcg
      361 gggagggcgg acggcatacc cgaatcctct cagcccgggc ggtcgaatca tgcgcggtcg
      421 aaacgcgcgc gggtcgaatc atgcgcgggt cgaatcgtgg acgggagtct tgcgcctcgg
      481 gaccgggacg acgctgcggc ggcgtgtcgc accgacacgc cgtgggtccc gcgaaagcct
      541 tcagaagtct cgcccaaccc cttgtgaacc ggcggtgacg ggactagaaa tggggatacg
      601 ccaacggttc gccggaggcc agcaaggcgc caccatcggc ccaaaactct ttcgggagtg
      661 gcggattcga ggtgctgagc gaagggtcgg acctcggtcc ggccacgctg cgagcgcagg
      721 ttcgcggcag cccgaccttc gggtcggagg cgtcgagctt cgcgcccgac gcacggtggt
      781 cggcttcggc cgagcgccag gaagggcccc ggaacctcat acgttccggg gcccttcttc
      841 ctgccccact gactccttga ccgctgcgct ggccgagccc ccagttgcct gcggggaaag
      901 cccgggctga gcacaaaacc cagtcactgc tcgcggtgca ctgccgctgc agacgcccgg
      961 cttcctagac tgcggacact cacatcaacg gcgaggaggg tggcgcacgc atggtggaga
     1021 tgacccgcgc tgacgcggtc cgcgagatcg ctgcggggta tgcccttcac cgaggccccc
     1081 tgctgcccgt gctgcacgcg gtgcaggacc ggttcggagc catcgagcgt ggcgacctgg
     1141 ttgccgtcgc cgatgtcctc aacctctcga tcgccgatgt ccacggcgtc atgaccttct
     1201 tccacgactt ccgcgccgct cccccgccga cgcacgtcgt cgcgatctgt cgcggcgagg
     1261 cctgccagtc ggtcggcgct gagcgactca tggccgacgt ccgcgccgac ctgcaggagg
     1321 catcggacgt cgaggtgcac gaggtcttct gtctgggcaa ctgcgctctc ggcccgagcg
     1381 gcaccgtcga cggtcgcctg ttcgggcacc tcaccgcttc gagggtgggc gcgtcgctgc
     1441 ccgggcggtc gtcatgaccc ggttcttcgt ccccggtgac gccgctgccg tgtcggtcgg
     1501 cgcggacgag gtcgctgcgg ccctgaccgg gcagcccggc gttgaagtcg tccgcaccgg
     1561 gtctcgcggg atgctctggc tggaacccct cgtcgaggtc gatacggccg acggtcgggt
     1621 gggcttcgcc aacgtctcac caggccaggt cggcgcgctg ctcgccgacg cgacctcggt
     1681 atgttcgcgc gagccaactg cttcggccgg gccggccccg gaagccgacc acccggcata
     1741 cctcggccct ctggacgagc acccgtggat gacccgacag gctcgcctga ccacggcgcg
     1801 cttgggcgtc gtcgacccgg ccgatcccga cgactacgcg cggcacggcg ggtgggtcgg
     1861 gctgcggcgg gcgctgggca tggcccccga ggccgttgtc gaggaggtcg tcgcgtcggg
     1921 gctgcgtggc cggggcggtg cgggcttccc cgccggggtg aagtggcgga ccgtcgcggg
     1981 cgggcccggg ccgctgaagt tcgtcaccat caacgccgac gagggcgact cgggcacgtt
     2041 cgccgaccgg atgctcatgg aaggagaccc cttcgcgctg atcgagggca tggcgatcac
     2101 cgcctgggct gtcggtgcga gcgagggcta cgtcttcatc cgttcggaat acccgcacgc
     2161 catcgccacg atgcgccgag ccgtcgagac ggcgcgaacg ggtgggtggc tcgggccgga
     2221 cattctcggg tcagggctgg cgttcgacgt ggaggtgcgg gtcggcgccg gcgcgtatat
     2281 ctgcggcgag gagacctcga tgctctccac catcgaaggt cgccgcggcg aggtgcgcgc
     2341 caagccaccg atccccgcgc tgtcggggtt gttcgggcga ccgaccgtcg tcaacaacgt
     2401 cctcacggtt gcggcggtcc cgtccatcct cgccgacggt cccgaggcgt atgccgccca
     2461 cggcgtcgac cgctcgcgcg gcacccaggt gttccaactg gccggcaacg tcgctcgtgg
     2521 cggcctggtc gaggtcgact tcgggatcac cctgcgcgat ctcgtcgagg gtttcggcgg
     2581 cggcacccgc tcgggacgtc cggtccgagc cgtccaggtg ggcggaccgc tgggggctta
     2641 cgtgcccgcc gatcgactgg acgtgccgct ggcctacgag gcgatggccg atgcgggttt
     2701 catgctgggg cacggcgggg tggtcgtctt cgacgacacc gtcgacatgg gccggatggc
     2761 ccgcttcgcc atggagttct gcgccaagga gtcgtgcggc aagtgcacgc cctgtcggat
     2821 cggctcgcag cgcggcgtcg agacgatcga ccggctcgtc gccgggggga ccgagtcgga
     2881 tcgccgggcg aacctggctc tcctgcaaga cctgtgcacg accatggaac gcggctccct
     2941 gtgtgcgatg ggcgggctga cgccgatgcc ggtgcgcagc gccctcacct atttccccga
     3001 ggatttcggg ctcagtgcgg acggagagcc ccgatgaccc tcgcccccga acgtgacctc
     3061 ggcacagcgg cggtggtcgg tgagccgacc gcgacggtga cgatcgacgg catacccgtg
     3121 cgggtcgccc ccggcacctc ggtgctgcgt gctgcttccc tcgccggggt cgagatcccc
     3181 cgcttgtgcg ccaccgatcg gctggaggcc ttcgggtcgt gccggctctg cctcgtcgag
     3241 gtcgacggcg ttcgtggcac tcccgcgtcg tgcaccaccc tgtgctccga cgggatgagc
     3301 gtggcgaccg acaccccgat ggtccggcag ctgcggcacg gggtgatgga gctctacctg
     3361 tccgaccacc cggccgactg cgcaggctgc gagcgcggct cctgcgacct gggccggctg
     3421 gcggcagacg ccggcgttgc cgaggttcgc tacggcctcg ggcgctccca cgccgacgcg
     3481 accaccgacg tgagcaaccc ctacttcgcg ttcgacccgg cggcctgcat cgtctgctcg
     3541 cgctgcgtgc gggcctgcgg tgaggttcaa ggcaccttcg cgctgagcgt cgcaggtcgt
     3601 ggcttcgact cgcagatcag ccctagcggc acggatttcc ttgcctccga gtgcgtttcg
     3661 tgtggcgcct gcgtgcaggc ctgccccacg tcggccctgg aggaaaagtc gctcgtttcg
     3721 ttggggatgc cctcgcgggc tgtggtgacc acctgcgcgt attgcggtgt cgggtgttcc
     3781 ttccgcgccg aggtgcgcga tgaccccgag gggacgacgc tcgtgcggat ggtcccgtgg
     3841 aaggacggcg gggccaacga gggtcactcg tgcgtcaag
//
LOCUS       DAOMZY010000326         3868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_22395_length_3868_cov_5.311828, whole genome shotgun sequence.
ACCESSION   DAOMZY010000326 DAOMZY010000000
VERSION     DAOMZY010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22395_length_3868_cov_5.311828"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="PLK64_14640"
     CDS             complement(<1..709)
                     /locus_tag="PLK64_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171961.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HPV81029.1"
                     /translation="MTDHDAAVGDPTSDLSEASCPVAHARPQHPTEGSGNRTWWPNQL
                     NLRILATNPPAGNPMAAGFDYAAAFGALDLPSVKRDIESVLTTSQPWWPADYGHYGPL
                     MIRMAWHSAGTYRVSDGRGGAGAGQQRFAPLNSWPDNANLDKARRLLWPIKKKYGRAL
                     SWADLMILAGNVALESMGFTTAGFGGGRADVWQADEEVYWGPEDTWLGDERYTGDRDL
                     ENPLAAVQMGLIYVNPEG"
     gene            complement(706..1188)
                     /locus_tag="PLK64_14645"
     CDS             complement(706..1188)
                     /locus_tag="PLK64_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009883574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPV81030.1"
                     /translation="MPAHTEVARALRDAGLRVTQPRLAVLAAVHDHPHSDTEGLIVAA
                     RAELAGVSHQAVYDVLRALTDAGLVRRIQPPGSIARYEARVGDNHHHLVCRHCASIVD
                     VDCAVGERPCLTASDDHGYVIDEAEVVYWGTCPACLAGSPPPSSSDTRSTHHPEGSEP
                     "
     gene            complement(1269..2177)
                     /locus_tag="PLK64_14650"
     CDS             complement(1269..2177)
                     /locus_tag="PLK64_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HPV81031.1"
                     /translation="MNLRDLQYLVAVADHRHFGRAATACAVSQPTLSTQLKKLEVELG
                     VQLVERGGRAVILTPVGDQVVARARAVLAEGEAIRRIARQAQDPRAGRLALGAFPTIA
                     PYLFPHVLGELRRQLPDVELLLVEDKTAALLTQLRDGSLDAVVVALPVDEEGLHAEPL
                     FREALLLAAPATHPLGRDTSPVGLEDLAGESVLLLSRGHCLREQALAVCRAAGAVERP
                     GFRATSLDTLRHMVEAGLGLTILPRLSLPEARELPPEVVVREFREPTPYRDLALVWRL
                     GSVRDALLPEVAQLLRRLPTGLVTPL"
     gene            complement(2233..3813)
                     /gene="ahpF"
                     /locus_tag="PLK64_14655"
     CDS             complement(2233..3813)
                     /gene="ahpF"
                     /locus_tag="PLK64_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HPV81032.1"
                     /translation="MLDPNLTAALKSYLERLTLPVELEASLDDSRASAQTRELLGEIA
                     ALSALVTVTSGDDARKPSFAVKRAGTDIQVRFAGVPLGHEFTSLVLALLQVGGVAPKI
                     SEETAAAIRAIEGEHHFETYMSLTCQNCPDVVQALNAMSVLNPRITHVTIEGGAFQGE
                     IESRKVLAVPTVYRNGELFGQGRMTLEEIVGKLDSGAAARDAQRLEAMDPFDVLVVGG
                     GPAGAAAAIYASRKGIRTGIVAERFGGQVMDTMAIENFISVTHTEGPKLAAALEEHVR
                     SYEVEVMELQRAAALTPAAEPGGLAQVRLDNGAVLSARTVILATGARWRHMGVPGEED
                     YRNKGVTFCPHCDGPLFKGKRVAVIGGGNSGIEAAIDLAGVVGHVTVLEFMDELRADA
                     VLQRKLYSLPNVEVILSAKTTEVLGNGEQVTGLDYEDRTTGASRHLDLEGIFVQIGLL
                     PNTEWLADALTLTPRREIVIDDRGQTSATGIFAAGDCTTTPYKQIVISLGAGATAALG
                     AFDHLIRSTAPEASLVDA"
BASE COUNT          577 a         1327 c         1390 g          574 t
ORIGIN      
        1 ggccttcggg gttgacgtag atcagaccca tctgcacggc ggccagcggg ttctccaggt
       61 cgcggtcgcc ggtgtagcgc tcgtctccga gccaggtgtc ctcgggcccc cagtagacct
      121 cctcgtccgc ctgccagacg tcggcccggc ccccgccgaa cccggcggtg gtgaacccca
      181 tcgactcaag ggcgacgttg ccggcgagga tcatcaggtc ggcccaggac aaggcgcgcc
      241 cgtacttctt cttgatcggc cacagcagcc ggcgtgcctt gtcgaggttg gcgttgtccg
      301 gccaactgtt gagcggcgcg aaccgctgct gtccggcccc ggcgccccct cgaccatcgc
      361 tgacccgata ggtcccggcg ctgtgccagg ccatccggat catcaacggc ccgtagtggc
      421 cgtagtcggc gggccaccac ggctgcgagg tggtcagcac cgactcgatg tcgcgcttga
      481 cgctcggcag gtcgagcgca ccgaacgccg ctgcgtagtc gaagcctgcg gccatggggt
      541 tcccggcggg cgggttggtc gccaggattc gaaggttgag ttggttgggc caccaggtcc
      601 ggttgccgga cccctcggtg gggtgctggg gacgggcgtg cgccacgggg cagctggctt
      661 cgctcaggtc ggacgtcggg tcgccaacag cagcgtcgtg atcggtcatg gctcacttcc
      721 ttcggggtgg tgggtggagc gggtgtcgga ggaactgggc ggggggctcc cggcgaggca
      781 cgccgggcag gtcccccaat agacgacctc agcttcgtcg atgacgtagc cgtggtcgtc
      841 ggacgcggtg aggcaggggc gctcgcccac cgcacagtcg acatcgacga tcgaggcgca
      901 gtgccggcag acgagatggt ggtggttgtc gcccacgcgg gcttcgtagc gggcgatgga
      961 ccccgggggt tggatccggc gcaccagccc ggcgtccgtc agcgcgcgca ggacgtcgta
     1021 gaccgcctga tgggacaccc ccgccagctc ggcccgagcg gcaacgatca agccctcggt
     1081 gtcggagtgc ggatggtcgt gaacagccgc caacacggcc agtcgcggtt gggtgacccg
     1141 cagcccggca tcgcgcaacg cgcgggccac ctccgtgtga gcgggcatag cgccactatc
     1201 ccccgttatc ttgaaccggt caagatctcg cggctgtcca gcttcccgga cgcgcagctc
     1261 ggctaccgct agagcggcgt caccaggccg gtcggcagcc ggcgcaagag ttgagccacc
     1321 tccggcaaca gggcgtcccg gacgctgccc agccgccaga ccagggccag atcgcgatag
     1381 ggcgtcggct cgcggaactc gcgcacgacc acctcgggcg gcaactcccg cgcttccggg
     1441 agcgacaggc ggggcaggat cgtcaggccg aggcccgcct cgaccatgtg ccgcaaggtg
     1501 tccaggctgg tcgcccggaa gccggggcgc tcgacggcgc cggccgcgcg gcatacggca
     1561 agggcctgct cgcgcaggca gtggccgcga ctgagcagca gcacgctctc ccccgcgagg
     1621 tcctccaacc cgaccggcga ggtgtcccgg ccgagggggt gcgtcgccgg cgcggccaac
     1681 agcagggcct cccggaagag cggttcggcg tgcaagccct cctcgtccac cggcagggcg
     1741 acgacgaccg catcgaggga tccgtcgcgc agttgagtga gcagggcagc ggtcttgtcc
     1801 tcgacgagca gcaactcgac gtcgggcagt tgcctgcgca gctcaccgag gacgtgcggg
     1861 aagaggtagg gcgcgatcgt ggggaacgcc ccgagggcca gccgcccggc ccgcgggtcc
     1921 tgagcctgac gggcgatccg gcggatcgct tcgccttcgg ccagcaccgc tcgggcccgg
     1981 gcgacgacct ggtcaccgac gggggtgagg atgacggccc ggccgccccg ctcaaccaac
     2041 tgaaccccca actcgacctc gagcttcttc agctgggtcg agagcgtcgg ctggctcacc
     2101 gcacacgccg tcgccgcccg tccgaagtgc cggtggtcgg ccacggcgac gagatattgc
     2161 aggtcacgaa ggttcatggc cgcctctgca ccgcctcagt atgccgccag ccggccggat
     2221 cgcctccggc catcaggcgt cgacgaggct ggcctccggc gccgttgacc ggatgaggtg
     2281 gtcgaaggcc ccgagagcag cggtcgcccc ggcgccgagg ctgatgacga tctgcttgta
     2341 gggggtcgtg gtgcagtcgc cggcggcgaa gatcccggtg gctgacgtct gaccgcggtc
     2401 gtcgatgacg atctccctgc gcggggtgag ggtgagcgca tcggcgagcc actcggtgtt
     2461 gggcagcaag ccgatctgga cgaagatccc ctcgagatcc aggtgcctcg acgccccggt
     2521 cgtgcggtcc tcgtagtcca agccggtgac ctgctcaccg ttgccgagca cctcggtcgt
     2581 cttggcgctg aggatgacct cgacgttggg caggctgtag agcttgcgct gcaggacggc
     2641 gtcggcccgc aactcgtcca tgaactccag gacggtgacg tgcccgacga cgccggccag
     2701 gtcgatggcg gcctcgatgc ctgagttgcc gccgccgatg acagcgaccc gcttgccctt
     2761 gaagagcggc ccgtcgcagt gcgggcagaa ggtgacgccc ttgttgcggt agtcctcctc
     2821 gcccgggacg cccatgtgcc gccagcgggc accggtggcg aggatgacgg tgcgagccga
     2881 gagcaccgcg ccgttgtcca ggcgcacctg cgcgagcccg cccggctccg cggctggggt
     2941 gagtgcagcg gcccgctgca actccatgac ctcgacctcg taggagcgca cgtgctcttc
     3001 gagggccgcg gccagcttgg ggccttcggt gtgggtaacg gagatgaagt tctcgatcgc
     3061 catggtgtcc atgacctgtc caccgaaccg ctccgcgacg atgccggtgc gaattccctt
     3121 gcgggaggcg tagattgccg cggccgcgcc agccgggcca ccaccgacga cgaggacgtc
     3181 gaagggatcc atcgcctcga gccgctgcgc gtcgcgagcc gccgctccgc tgtcgagctt
     3241 gccgacgatc tcctcgaggg tcatccggcc ctgaccgaac aactcgccgt tccgatagac
     3301 ggtcggcacc gccagcacct tgcgcgactc gatctcgccc tggaaggccc caccctcgat
     3361 ggtgacgtgg gtgatccgcg ggttgaggac gctcatcgcg ttgagcgcct gcacgacgtc
     3421 ggggcagttc tggcaagtga gggacatgta ggtctcgaag tggtgctccc cctcgatcgc
     3481 ccggatcgcc gccgcagtct cctccgagat cttcggggcg acgccgccaa cctgcagcag
     3541 ggcgaggacc aacgacgtga actcgtgccc gagcggcacg ccggcgaagc gcacctggat
     3601 gtcggtgccg gcccgcttga cggcgaagga cggcttgcgg gcatcgtcgc cggacgtcac
     3661 cgtaaccagc gcggacaacg ccgcgatctc ccccagcaac tcacgcgtct gggccgacgc
     3721 gcgggaatcg tcgaggctgg cctccagctc gaccggcagg gtgagccgct ccaggtagga
     3781 cttcagcgcg gcagtgaggt tcgggtcgag catggtcggc tcctagcggg gcgcgggcga
     3841 acgtcggagg tgcgggagaa gccatccg
//
LOCUS       DAOMZY010000327         3851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_22534_length_3851_cov_6.011328, whole genome shotgun sequence.
ACCESSION   DAOMZY010000327 DAOMZY010000000
VERSION     DAOMZY010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22534_length_3851_cov_6.011328"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1934)
                     /gene="secA"
                     /locus_tag="PLK64_14660"
     CDS             complement(<1..1934)
                     /gene="secA"
                     /locus_tag="PLK64_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPV81033.1"
                     /translation="MAKVFEKLLRAGEGRVVKRLEAVAAQVNILEEDFEKLTDAELRA
                     ETDTFKRRLAEGESLDAILPEAFAAVREASKRTLGKRHFDVQIMGGVALHQGNVAEMR
                     TGEGKTLVATLPTYLNALAGKGVHVITVNDYLAEYQSELMGRIHRMLGLETGCILSAM
                     TPDQRRAEYAKDITYGTNNEFGFDYLRDNMAWSTDELVQRGHHYAVVDEVDSILIDEA
                     RTPLIISGPADQATKWYVEFAKIVQRLNRGELESGEGDYEVDEKKRTIGVLEQGIAKV
                     EDLLGIDNLYESVNTPLIGFLNNAIKAKELFKRDKDYVNMDGEILIVDEHTGRMLPGR
                     RYNEGMHQAIEAKEGVEIKNENQTLATITLQNYFRMYSKLAGMTGTAQTEAAELNSIY
                     KLGVITIPTNMPMIRMDQADLIYRTADAKFDAVVEDIVERHAEGQPILVGTVSVEKSE
                     HLSSLLRRRGVRHEVLNAKQHAREASIVAEAGRKGAVTVATNMAGRGTDIMLGGNSEF
                     RAVQELKRRGLDPEETPQEYEDAWDDALAAAEAAVAKEHDEVKSLGGLYVLGTERHES
                     RRIDNQLRGRSGRQGDPGESRFYLSLQDELMLRFNAGMVDRVMQTARIEDDVPIESKI
                     VSRSIAGAQSQVEAQNYEIR"
     gene            complement(2006..2839)
                     /locus_tag="PLK64_14665"
     CDS             complement(2006..2839)
                     /locus_tag="PLK64_14665"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPV81034.1"
                     /translation="MATATPTASSPPTSVEGGFAEHGALVAALTALPRGKNVAFGVGV
                     LDAATGRRFVFDVGAPFEMASTVKVDILVATFLRAADAGRGLTAGEKSLASAMIRNSD
                     NAAASTLFRAAGRAPGMAKAYQRMGMTATKASSAWGLTTTTPGDRLVVLEALARGGGA
                     LTAEAGAEILALMRSVTTSQRWGVGGVAGAGETVAVKNGWLPRSNQGGAMGHLDLRPA
                     HRALDRPAAGRVQPGACQPGRRYRFRREGADAGARGIEGLTGSHVRGTRATARGVGEV
                     T"
     gene            3247..>3851
                     /locus_tag="PLK64_14670"
     CDS             3247..>3851
                     /locus_tag="PLK64_14670"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPV81035.1"
                     /translation="MPPAPLPADPSASPAVTYPDCVPELSDGVVTLRAHRPQDAERIV
                     QQCVDGDSRAFLPLPSPYGLPEAQAFLDKVAAAWLDEWGAREWAITDAADPSGQFLGS
                     INLHERSPYRAEVGFGLHPEGRGRGLVARAVRLLAARAFDEGAEVLRWRAVAGNWASR
                     RVAWACGFEAPTTLIGGRLDSDGHPGDEWHAVLRPGEPMAPR"
BASE COUNT          560 a         1361 c         1285 g          645 t
ORIGIN      
        1 ttgcggatct cgtagttctg cgcctcgacc tggctctggg cgcccgcgat cgaacggctg
       61 acgatcttgg actcgatcgg gacgtcatcc tcgatccggg cggtctgcat gactcggtcg
      121 accatgccgg cgttgaaccg gagcatgagt tcgtcctgca gggacaggta gaaccgcgac
      181 tcccccgggt caccctgacg tccggaccgg ccgcgcaact ggttgtcgat gcgccgcgac
      241 tcgtgccgct cggtgccaag gacgtagagg ccgcccagcg acttgacctc gtcgtgctcc
      301 ttggcgacgg ctgcttcagc cgcagccaac gcgtcatccc acgcgtcttc gtactcctgc
      361 ggggtctcct ccgggtccag gccacggcgc ttgagctcct ggacggcccg gaactcggag
      421 ttgccgccca gcatgatgtc ggtgccacga ccggccatgt tggtggcgac cgtgacggcg
      481 cccttacggc cggcctcggc gacgatcgat gcctcacggg cgtgctgctt ggcgttgagc
      541 acctcgtggc ggaccccgcg ccgacgcaac aggctcgaca ggtgctcgct cttctcgacc
      601 gacaccgtgc cgacgaggat cggctggccc tcggcgtgcc gttcgacgat gtcctcgacg
      661 accgcgtcga acttcgcgtc ggcggtgcgg tagatgaggt cggcctggtc catccggatc
      721 atcggcatgt tcgtcgggat cgtgatgacg cccagtttgt agatcgagtt gagctcggca
      781 gcctcggtct gggccgtgcc cgtcatgccg gcgagcttgg agtacatccg gaagtagttc
      841 tgcagggtga tcgtcgcgag cgtctggttc tcgttcttga tctcgacgcc ctctttggcc
      901 tcgatggcct ggtgcatccc ctcgttgtac cgacggcccg ggagcatgcg cccggtgtgc
      961 tcatcgacga tgaggatctc accgtccatg ttcacgtagt ccttgtcccg cttgaacaac
     1021 tccttggcct tgatggcgtt gttgaggaag ccgatgagcg gggtattgac cgattcatac
     1081 aggttgtcga tgcccagcag gtcctcgacc ttggcgatgc cctgctcgag gacgccgatc
     1141 gtgcgcttct tctcgtcgac ctcgtagtcg ccttcgccgg actccaactc gccacggttg
     1201 agccgctgga cgatcttggc gaactcgaca taccacttgg tcgcctgatc ggccggcccc
     1261 gagatgatga gcggcgtccg ggcctcgtca atgaggatcg agtcgacctc gtcaacgacg
     1321 gcgtagtggt gcccgcgctg gaccaactcg tccgtcgacc aggccatgtt gtcgcgcaag
     1381 tagtcgaagc cgaactcgtt gttcgtgccg taggtgatgt ccttggcata ttcggcgcgg
     1441 cgctggtcgg gggtcatcgc ggagaggatg cagccggtct ccagcccgag catccggtgg
     1501 atgcggccca tcaactccga ctggtactcg gccaggtagt cgttgaccgt gatgacgtgg
     1561 acgcccttgc ccgcgagcgc gttgaggtag gtcggcaggg tcgcgacgag ggtcttgccc
     1621 tcaccggtgc gcatttcggc gacgttgccc tggtgcaggg caaccccgcc catgatctgc
     1681 acgtcgaagt gccgcttgcc gagggtccgc ttggaggcct cccgaaccgc ggcgaaggcc
     1741 tcgggaagga tcgcgtcgag cgactcgccc tcagccaggc gccgcttgaa ggtgtcggtc
     1801 tcggcgcgca actcggcgtc ggtcagcttc tcgaagtcct cctcgaggat attgacctgg
     1861 gccgccaccg cttcgagccg cttgacgacc cggccctcgc cggcacgcag caacttctcg
     1921 aacaccttgg ccacaagaaa ctcctcgaac gacgaacggc ggtcggctca cgtagaacca
     1981 gagacccagg gtagtgcccg ggctctcagg tcacctcacc aacgcctctt gccgtcgccc
     2041 tggttccgcg cacgtgcgag cccgtcagac cttcaattcc ccgcgcgcca gcgtcagcac
     2101 cttctcgacg aaatcgatac cggcggcctg gctggcatgc ccccggctgc acacggccag
     2161 ccgcaggtcg gtcgagggcc cggtgagcag gccggaggtc gagatgaccc atcgcccccc
     2221 cctggttgct ccgaggcagc cagccgttct tgaccgcgac cgtctcaccg gccccggcca
     2281 ccccgcccac gccccaccgt tggctggtcg tcaccgagcg catcagcgcg aggatctcgg
     2341 cgccagcctc ggccgtcagc gccccaccac ctcgcgccag ggcctcaagg acgaccaacc
     2401 ggtcgcccgg cgtcgtcgtt gtcaggcccc aggccgacga ggctttcgtc gccgtcatcc
     2461 ccatgcgctg gtaggccttg gccatccccg gcgcccgtcc ggccgcgcgg aacagtgtcg
     2521 atgccgccgc gttatcggag ttgcggatca tcgccgacgc gaggctcttc tcgccagccg
     2581 tgagaccccg acctgcgtcg gcggcgcgta ggaacgtcgc cacgagaatg tccaccttga
     2641 ccgtgctggc catctcgaac ggcgctccca cgtcgaagac gaaccgtcgc ccggtcgcgg
     2701 cgtccaagac gccgacgccg aaggcgacat tcttcccccg cggcaaggcg gtcaaggcgg
     2761 cgacgagagc gccgtgctcg gcgaacccgc cctcgaccga cgtgggagga gaggaggccg
     2821 tcggggtggc tgttgccacc gggctcgtcg ccgaagcagt cactgtcgcg gtcgcgggcg
     2881 gcaccgttgc ccgggccgct gtcgtaacct gagaggtcgt cggggtggcg accggcgtct
     2941 gcgcggccgg ggccgatccc ggcgaccgat cggtgagcac ttcgacaagc ccgcccacca
     3001 gcgcacggcc atcgcgacgc gacgagtcac gtgggcgcct gccagagccg acggagcgag
     3061 cgcctgaccc cgggcgcgat ggcgcccccg ggacggctgc ggcgcggagg acggcacgtc
     3121 ggacacggcg caagtatggc gacacctgct gtgtcggcgc gcctcggggc caacccagca
     3181 acccccgacc accgcggttg agcaaagcgg ttgctcaacg gcataccggc tcgacaccct
     3241 tggggtatgc cgcccgcgcc ccttcctgcc gacccctccg cttcgcccgc agtgacctac
     3301 ccggactgcg tcccggagtt gtccgacggc gtcgtcaccc tgcgcgcgca ccgaccgcag
     3361 gacgccgagc gcatcgtgca gcagtgcgtg gacggcgaca gccgggcctt cctcccgctt
     3421 ccctccccct acgggttgcc cgaggcgcag gcattcctcg acaaggtcgc tgcggcctgg
     3481 ctcgacgagt ggggcgcccg ggaatgggcc atcacggacg cggccgaccc gagcgggcag
     3541 ttcctcggca gcatcaacct gcacgagcgc agcccctatc gcgccgaggt cggtttcggg
     3601 ctgcaccccg agggacgcgg gcgcgggctg gtcgcccggg cggtccgact gttggcggcc
     3661 agagccttcg atgagggagc ggaggtgctc cggtggcgag cggtcgccgg caactgggcc
     3721 tcccgcaggg tggcctgggc ctgtggcttc gaggccccga cgaccctgat cgggggacga
     3781 ctggacagcg acgggcatcc cggcgacgaa tggcatgccg tgctgcgccc cggcgagccg
     3841 atggccccgc g
//
LOCUS       DAOMZY010000328         3845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_22589_length_3845_cov_5.498417, whole genome shotgun sequence.
ACCESSION   DAOMZY010000328 DAOMZY010000000
VERSION     DAOMZY010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22589_length_3845_cov_5.498417"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..1330
                     /locus_tag="PLK64_14675"
     CDS             104..1330
                     /locus_tag="PLK64_14675"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HPV81036.1"
                     /translation="MDASPAAGGSRREGPLHGIRVLELGTMYAAPTCGRMLRDFGAEV
                     TKVEDPGAGDYARQWTPMKNGQSMGFARLNAGKRSVGIDVRSEAGRDLVRRLAADVDV
                     VIESFRPGRLEAWGLGYDVLSVDNPRLVLTRVSGFGQTGPYSGKPGFGTVAETGSGFA
                     FINGWPETPPTSPPFGFADSIAGISAAMGTAMALYRRERTGAGDVVDVALYEPLMFII
                     GDMIINYTGTGFVQGRVGNGTGSASPRGVYQAADGKWLSIAASNQGIAIRLFHAMGRP
                     DILEDPRFATNAARMEHNDAIQAIVSAWVAARPRHEVLAVLDEHEVVCSQVNDASDIV
                     ADPHFSERTLVELTGSEALGRVLTPGPILHMGGYDGPVYAGVPTIGEHTDEVLLAAGL
                     GQAELDRLRSEGVIGS"
     gene            complement(1517..1651)
                     /locus_tag="PLK64_14680"
     CDS             complement(1517..1651)
                     /locus_tag="PLK64_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81037.1"
                     /translation="MGWVWLAVDSGRSLDPYAGVEMSLNDGTPIAQRFVATAWELGAA
                     "
     gene            complement(1736..1969)
                     /locus_tag="PLK64_14685"
     CDS             complement(1736..1969)
                     /locus_tag="PLK64_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81038.1"
                     /translation="MDTSLIRMASEIRESWPFALYEPGDPPVTVQVWERLGLLPTERS
                     GGVHQPTEAPLERYAEASLRRLDGCLEAGHFEP"
     gene            2108..2839
                     /locus_tag="PLK64_14690"
     CDS             2108..2839
                     /locus_tag="PLK64_14690"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HPV81039.1"
                     /translation="MRVGRPSKETMGFTELGQAVRARRRTAGMTRADLANRSGLSANT
                     VMKVEQGQTRDPGVFKVAALCRALSVSIDDLVTEAERNELAWEEPTMVNGIVSVGYEG
                     RQIDDFVAGLQRAGVRTVADVRLNAISRKPGFSKTRLRDALAAVGIGYVHMRSLGNSK
                     NNRQPFWDGHPEEGRRVFREALQSTGAQASLGELSQLARDHVVAVLCFEADVERCHRK
                     VIIDQVVKDTATPVADLPAYESLRG"
     gene            complement(2818..3663)
                     /locus_tag="PLK64_14695"
     CDS             complement(2818..3663)
                     /locus_tag="PLK64_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81040.1"
                     /translation="MVTLFGDDFGHPVEKPIAPARHRVLITVKAAPNPSATHGETVCV
                     AGVILGDLGATGWIRLYPINFRHLPQATEQFRKYDIVSVDCRPAGEARLESWQPNMAT
                     LRVESFLPPWRRRRDIIDPLIEVSMCGLRNRAKADAQAPSLALVRPAEILGFRTERHP
                     GWSRDEQAKIDAYVNQLELDVFEEAQDKTPLTAPRFRGVYKWRCSEPSCGTHEQSIID
                     WEFVAFQRHLWNRSDADLQRELHRRFFEEICSAKNDVAFYVGNQAKRPQTFSILGVYY
                     PRRDS"
BASE COUNT          667 a         1209 c         1319 g          650 t
ORIGIN      
        1 tccagtggcg gctctgatgc cggacggggc gactgtgaca gcggggtctg cgggcggggc
       61 ggctgacccg tcggcggagc ggtcgactgc gaggggcgcg tccgtggacg cgagcccggc
      121 ggcaggtggg tcgagacgcg aaggcccgct gcacggcata cgcgtgctcg aactgggcac
      181 gatgtatgcc gcgcccacct gcgggcggat gctgcgcgac ttcggcgcgg aggtgaccaa
      241 ggtcgaggac ccgggcgcgg gcgactatgc gcgccaatgg actccgatga agaacggcca
      301 gtcgatgggg ttcgcgcggc tcaatgccgg caagcgctcg gtgggcatcg acgtgcggtc
      361 ggaggccggg cgcgatctcg tgcggcgact cgcggccgac gtcgatgtcg tcatcgagtc
      421 gttccggccg gggcggttgg aggcgtgggg gctgggctat gacgtgctgt cggtcgacaa
      481 cccgaggctg gtgctcacgc gggtgtcggg cttcggacag accgggccat atagcgggaa
      541 gccaggcttc gggacggttg ccgagacggg cagcggcttt gccttcatca acgggtggcc
      601 ggagactccg ccgacgtcgc cgccgttcgg tttcgccgac tcgatcgccg gcatctcggc
      661 ggcgatgggg acggcgatgg cgctctatcg acgcgaacgc accggggctg gtgacgtcgt
      721 cgatgtggcg ctctatgagc cgctgatgtt catcatcggc gacatgatca tcaactacac
      781 ggggacgggg ttcgtccagg ggcgggtcgg caacggcacc ggctcggcgt cgccgcgcgg
      841 ggtctatcag gccgccgacg gcaagtggct ctcgatcgcc gcgagcaacc agggcatcgc
      901 catccggctg ttccacgcga tgggtcggcc ggacatcctc gaggacccgc gcttcgccac
      961 caacgccgcc cggatggagc acaacgacgc catccaggcg atcgtcagcg catgggtggc
     1021 agcgcggccg cggcatgagg tgctcgcggt cctcgacgag catgaagtgg tctgctcgca
     1081 ggtcaacgac gcgagcgaca tcgtcgcgga cccgcacttc tcagagcgca ctttggtgga
     1141 gctgacgggg agcgaggcgc tcggacgggt gctcacgccc ggcccgatcc tgcatatggg
     1201 tggttacgac ggccccgtgt atgccggtgt ccccaccatc ggggaacaca ccgacgaggt
     1261 gctactcgcc gccgggttgg gccaggccga gctcgaccgg ctgcggtccg aaggcgtcat
     1321 cggcagctga gtccgtggct ggactgggct gggctggggg gttggctgag ctgggctggc
     1381 ggccgttgct ggggagttgg gaactcgggc cgggtcagcg tgcttggtgt gccgtcgctt
     1441 gcatgaggct agtgacgctt ggatgaggct ctaccggcat acaagcgtca ctagcctcat
     1501 acaagcgagc ggcgggtcag gccgcgccca gttcccacgc ggtggccacg aagcgctgag
     1561 cgatcggcgt cccgtcgttg agcgacatct cgactccggc gtaggggtcg aggctgcgcc
     1621 cgctgtcaac cgctagccac acccagccca tgggctcaag cagccagtgg gctccgggca
     1681 ggaccccgat caggaagtag cacaggtaga tcgagatggc tcgagccgcc tccgatcaag
     1741 gctcgaaatg acccgcttcg agacagccgt cgagccgacg aagggacgct tcggcatacc
     1801 tctccagagg cgcctcggtc ggctggtgga cgccgccgga ccgttcagtt ggcagcagcc
     1861 ccaatcgctc ccacacctgc acggtcacag gcgggtcgcc cggctcgtag agcgcgaagg
     1921 gccaagactc gcggatctca ctcgccatcc ggatcaggct ggtatccacc agttgaggct
     1981 acaactggat gggggatcac ctcgcctttg ccgcccaaag gctgagtccg acagcctcat
     2041 gggcaacgga gctggtgccc cagaacaggt tgcaggatga gtaaatattt actgctagtc
     2101 tcacaacatg cgcgtcggtc gtccctcgaa ggagacgatg gggttcactg aactcggtca
     2161 ggccgtgcgc gcccgacggc ggacggcggg gatgactcga gcggacctag caaaccggtc
     2221 tggactgtca gccaacacgg tgatgaaggt tgagcagggc cagacccgcg accccggcgt
     2281 tttcaaggtc gccgcactgt gccgtgccct gtccgtgtcg atcgacgatc tcgtcactga
     2341 ggccgaacgc aatgagctcg cctgggagga accaaccatg gtcaatggaa tcgtctcggt
     2401 cgggtacgag gggcggcaga tcgacgactt cgtcgccggc ctgcaacgag cgggggtgcg
     2461 caccgtcgcc gacgttcgcc tcaacgccat cagccgcaag ccgggattct cgaagactcg
     2521 gctccgggac gccttggccg ccgtcggcat cggctacgtc cacatgcgca gcctcgggaa
     2581 ctcgaagaac aaccgccagc cgttttggga tggtcacccg gaggaagggc ggagggtgtt
     2641 ccgagaggct ctgcaatcga cgggcgccca agcctccctt ggagagcttt cccagttggc
     2701 ccgcgatcac gtggtcgcgg tgctctgctt cgaggccgat gtcgagaggt gtcatcgcaa
     2761 ggtcatcatc gatcaagtag tgaaggacac agccaccccc gttgccgatc tccccgccta
     2821 cgaatccctt cgcgggtagt agacgccgag gatgctgaag gtttgtggtc gcttcgcctg
     2881 attgcccacg tagaacgcaa cgtcgttctt cgccgaacag atctcctcga agaagcggcg
     2941 gtggagctcc ctctgtaagt ccgcgtctga tcgattccac aggtgccgct ggaaggcaac
     3001 gaactcccag tcgataatcg actgttcgtg ggtgccgcac gatggttcac tgcagcgcca
     3061 cttgtagaca cccctgaacc gaggagccgt cagtggcgtc ttgtcctggg cttcctcgaa
     3121 aacgtcaagc tcaagctgat tgacgtaggc gtcgatcttt gcctgctcat cccgactcca
     3181 tccagggtgt cgttccgtcc tgaacccgag gatttcagct ggtcgaacga gcgccagaga
     3241 tggcgcctgg gcgtcggcct tggcgcggtt gcgaagaccg cacatcgaca cctcaatgag
     3301 cggatcgatg atgtcccgac gcctcctcca cggcggcagg aagctctcca cacgcagggt
     3361 cgccatgttg ggttgccagg actccaaccg agcttcgccg gcgggccggc agtcgacaga
     3421 aacgatgtcg tacttccgga actgttctgt cgcctgcggc aggtgccgaa agttgatcgg
     3481 atagaggcga atccagcctg tcgcgcccag gtctcccagg atgacgccgg caacacagac
     3541 cgtctcacca tgcgtagcag acgggttagg agctgccttg acggtaatca agacccggtg
     3601 tctcgcgggc gcgatcggct tctccaccgg atgaccaaaa tcgtcgccga acaacgtcac
     3661 cataatgcgc ggcacctccc ctgtcggttg tcagccacgc taaccgcggc cactgacatc
     3721 tccaggctgc cgccgcggag tcgctcgggg ggtcgcggct tggatgaggc tggtgacgct
     3781 tggatgaggc tctaccggca tacaagcgtc accaacctca tacaagcgag cgcggcgccc
     3841 gcccg
//
LOCUS       DAOMZY010000329         3829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_22732_length_3829_cov_4.141494, whole genome shotgun sequence.
ACCESSION   DAOMZY010000329 DAOMZY010000000
VERSION     DAOMZY010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22732_length_3829_cov_4.141494"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLK64_14700"
     CDS             complement(<1..255)
                     /locus_tag="PLK64_14700"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HPV81041.1"
                     /translation="MQLRELLGVLRARWRTVAACLLVVVGLTAAVTVTTTPNYTASAR
                     VYLTVERPADLARGDAGGVSVMRYNDLRTLAEVLYSPTVME"
     gene            392..2452
                     /locus_tag="PLK64_14705"
     CDS             392..2452
                     /locus_tag="PLK64_14705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPV81042.1"
                     /translation="MSHSAVVDAWRERERLLRRDGLDVRTISARVWDEAGMPVRLVPR
                     PGEPVEGVATIGRHPALFLFDPRPLWRALGERWDVIDLHEEPFALATAEVLLLLAVRA
                     RHTPYLLYSAQNLDKRYPLPFRVLARRTLRHAAGVSVCNAAAGDLVVRRGFPGRPQVI
                     PLGVDPAVFAPDPARCPPTPGGPVVVGYAGRLAAHKGVDVLLDAAATQPRLTLRLAGS
                     GPDEAGLRERVTALGLAERVSFVGALDTDALADFYRGLDVLAVPSLTTDGWVEQFGRV
                     AVEAMACGIPVVASDSGALPDVVGGAGRLVPPGDAAALGRALVTICDDPTEWASLRAN
                     GFARSQEATWGAVAARYRRLYHLAVHPGHAAHSAQGAHPTEARAVEVIVVAYGTPELL
                     GRALASVPGLPVTVVDNSGSADVERVCREAGVRYLDPGANLGFGGGVNVGLADRALPG
                     ADVLLLNPDAALTPGAVAALSARLAADAGLASVGPRQVDETGREARVGWPFPTPARSW
                     VVALGISRALGLVLGRRPGRAGGDGQPGQGAERIDYAIGSVLLLRAEALEQVGGFDED
                     FFLYAEETDWAKRAALLGWRHTVVAEASALHAGAATSVDTAARETHFAAGLERYLRKH
                     HGAGGWAVARSGEVVGAAVRAVVLPGDRGAAARARLRLLRTGPLRAERTLVPSRRRPR
                     VSAP"
     gene            2449..3777
                     /locus_tag="PLK64_14710"
     CDS             2449..3777
                     /locus_tag="PLK64_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HPV81043.1"
                     /translation="MTTVWRVGQWTLRASGVVVAVGLIAIAGLLVPSQPLVSAGIALG
                     ALALGLTLLDPIALPTLAIPALLVVSRVGAGGTNLAMSDFVLAAAFIPAVLLCQRPVS
                     REFASVIGLNAVYQFATLLTVVANPYLANVVEWFHAWMLVSGALFVGWAIGRGGRGPL
                     AMKLLIAGTIVMAVGTLATALVQYAKGDFGPVYPAWPYPMHKNFAGTVLGLAAVVVYL
                     RPSWFGWSARLANWLLVILLAGVAVTLSRQALLGLVVVFFLVVIWPRVDRRRSRAVLV
                     VGAALLGVVGVMVQDQLQSGNRFNSTLQRVDWFQSSATVWLTDPWLGVGLRWWYTDRF
                     EARFQPPNIELELLTSAGVVGLVAFFVMFGGTLHVLRRIDPAYGLLPSMVLICRLAQA
                     QFDLFWAAVLGSLPWLIVGLALGALAHDQAERAENQVRDLVAPKEHGVTR"
BASE COUNT          456 a         1307 c         1383 g          683 t
ORIGIN      
        1 ctccatgacg gtcggggagt agagcacctc ggcaagtgtc ctgaggtcgt tgtagcgcat
       61 gaccgacact ccgccggcgt cgccgcgggc taggtcggcg ggtcgctcga ccgtgaggta
      121 gacgcgcgcg gacgccgtgt agttcggcgt cgtcgtcacc gtcaccgcgg cggtgaggcc
      181 gacgacgacg agcaggcagg ctgcgacggt cctccaccgc gcgcggagga cgccgagcaa
      241 ctctcgcaac tgcacgtcga tgcactcccc ggcgtcgaga aacccgagcg ctcatcctcc
      301 cacacctggt ccgaactgcc ctgaattcga tgaaagtcgc tctaggctga ctcacaagac
      361 gtgtgggagg gcatcggtgc gggttctgcg ggtgagccac agtgccgtcg tcgacgcctg
      421 gcgcgagcgc gaacggctgt tgcggcgcga cggcctcgac gtccggacca tctcggcgcg
      481 ggtctgggac gaggccggta tgccggtccg tctggtcccg cgccccggcg agccggtcga
      541 gggggtcgcc accatcggcc gccaccccgc cctgttcctc ttcgacccgc ggccgctgtg
      601 gcgggcgctg ggggagcgtt gggacgtcat cgacctgcac gaggagccgt tcgccctggc
      661 caccgccgag gtgctgctgc tgctcgcggt gcgcgcgcgg cataccccct atctgctcta
      721 tagcgcgcag aacctcgaca agcgctaccc gctgcccttc cgggtgcttg ccaggcggac
      781 gctgcggcac gctgccggag tgagcgtgtg caacgccgcg gccggcgatc tcgtggtgcg
      841 gcgcgggttt ccgggtcgcc cgcaagtgat cccgctcggc gtggaccccg ccgtcttcgc
      901 ccctgacccc gcgcgttgcc ccccgacccc gggcggtccc gtcgtcgtcg ggtatgccgg
      961 tcggctggct gcgcacaagg gcgtcgacgt gctgctggac gcggcagcga cccagccccg
     1021 gctgacgctg cggctggctg gatccgggcc cgacgaggca ggcctgcgcg agcgggtgac
     1081 cgcgctgggg ctggccgagc gggtgagctt cgtcggcgcg ctcgacacgg atgcgttggc
     1141 cgacttctat cgcgggctgg acgtgctcgc cgtcccgtcg ctgacgactg acggctgggt
     1201 ggagcagttc gggcgggtcg ccgtcgaggc gatggcgtgc ggcatacccg tggtggcgag
     1261 tgacagcggc gccctgcctg atgttgtcgg cggggccggt cggctggtcc ctccggggga
     1321 cgcggcggcg ttgggccgcg ccctggtgac gatctgtgac gaccccaccg agtgggcatc
     1381 cttgcgggcc aacggattcg cccgctcgca agaggcgacc tggggtgcgg tcgcggcccg
     1441 gtaccggcgg ctctaccacc tcgccgtcca ccctggccat gcggcccact ctgcccaagg
     1501 cgcccacccg accgaggctc gggccgtcga ggtcatcgtc gtcgcctacg ggacccccga
     1561 gttgctcggc cgggccctcg cctcggtccc ggggctaccc gtgaccgtcg tcgacaactc
     1621 cgggtcggcc gacgtcgagc gggtctgccg cgaggccggc gtccgatacc tcgacccggg
     1681 agccaacctc ggcttcggcg gaggcgtcaa cgtcgggctg gccgatcggg cgcttccggg
     1741 cgccgacgtg ctcttgctca accccgatgc ggccctgacc cccggcgcgg tggccgcgct
     1801 gtcggcgcga ctggccgccg atgcggggct ggcgagcgtc gggccccggc aggtggacga
     1861 gaccggacgg gaagcgcggg tcggctggcc gttcccgact cccgcgcgca gttgggtcgt
     1921 cgcactgggg atctcgcgag ctcttggtct tgtgctcggt cgtaggcctg gtcgggccgg
     1981 gggcgatggg cagccagggc agggggccga acgcatcgac tacgccatcg gctcggtgtt
     2041 gctgctgcgg gccgaggccc tcgagcaggt gggcggcttc gacgaagact tcttcctcta
     2101 tgccgaggag accgactggg ccaagcgggc cgccctgctg ggctggcggc ataccgtcgt
     2161 cgccgaggcg agcgccctgc acgccggagc ggcgacgagc gttgacacgg cggcacgtga
     2221 gacgcacttt gctgccgggc tcgaacgcta cttgcgcaag caccatggcg cggggggctg
     2281 ggccgtggcc cgctccggcg aggtcgtcgg ggccgcggtg cgtgcggtcg tgctccctgg
     2341 ggaccgagga gcggcggcgc gcgcgcggtt gcggttgctc cgcaccggac cgctgcgagc
     2401 cgaacggacg ctggtccctt cgcgccgccg accccgggtg agtgccccat gacgacggtg
     2461 tggcgggtgg gccaatggac gctccgcgcg tcgggcgtgg tcgttgccgt gggcctgatc
     2521 gccattgccg gcctgctggt cccgagccaa cccctggtat cggcgggcat cgctttgggc
     2581 gcgttggcgc tggggctgac cctgctcgat ccgatcgcct tgccgaccct tgccatcccc
     2641 gccctcctcg tggtttcacg tgtcggcgcg gggggaacga acctggccat gtccgacttc
     2701 gtgcttgcgg ctgccttcat tcccgcagtg ctgctgtgcc agcgtccggt gagtcgggag
     2761 ttcgcttccg tcatcgggct taacgcggtc tatcaattcg caacgctgct gaccgttgtg
     2821 gcgaacccct acctggccaa tgtcgtggag tggttccacg cgtggatgct cgtcagcgga
     2881 gccctgttcg tcgggtgggc gatcggccgc ggtggccgtg ggccactggc catgaaactc
     2941 ttgattgccg gcactatcgt catggctgtg ggaaccctgg caactgctct cgtgcagtac
     3001 gccaaaggag atttcgggcc cgtctatcca gcctggccct accccatgca caagaacttc
     3061 gccgggacgg tcctggggtt agctgccgtt gtcgtctatc tgcggccgag ctggttcggg
     3121 tggagcgccc gactggccaa ctggctgctc gtcatcctgt tagccggggt cgccgtgact
     3181 ctttcgcgtc aggctctgct gggtttggtc gttgtcttct tcctcgtcgt catctggcca
     3241 cgcgtggacc gccgccgctc ccgggcggtc cttgtggtcg gcgctgccct ccttggcgtt
     3301 gtcggggtca tggttcagga ccagctgcag tccgggaacc gcttcaactc gacgcttcag
     3361 cgggtggact ggttccagtc ctccgcaact gtctggctga ccgacccgtg gctcggagtc
     3421 ggcttgcgat ggtggtacac cgaccggttc gaggcgcggt ttcaaccacc caatatcgag
     3481 ttggagttgc tcacgtcggc aggtgtcgtc gggctcgttg ccttcttcgt catgttcggc
     3541 ggcactctcc acgtgctgcg ccggatagac cctgcgtacg gtctccttcc gtccatggtg
     3601 ctcatctgtc gactagccca ggcgcagttc gatctgttct gggccgcagt gctcggatcg
     3661 ctcccgtggt tgatcgtggg gctcgccctc ggtgcgctgg cccacgacca ggcggaacga
     3721 gcggagaacc aggtgcgcga cctcgttgct cccaaggagc atggggtgac caggtgatcc
     3781 gcgtcctcca agtcacaccg gaaattggcc ccggaacggg cgtcggctc
//
LOCUS       DAOMZY010000330         3818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_22820_length_3818_cov_5.014882, whole genome shotgun sequence.
ACCESSION   DAOMZY010000330 DAOMZY010000000
VERSION     DAOMZY010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22820_length_3818_cov_5.014882"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PLK64_14715"
     CDS             <1..310
                     /locus_tag="PLK64_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81044.1"
                     /translation="LDDLGWGDPAWDLARPAGFWAAGLLSTDDWEGFLAAYRAAGGPG
                     VPVTGDPWPALDLPARAAVVIAATRAVGAVGAVGARRSGADTHSDPTAEALLDACRKM
                     "
     gene            320..667
                     /locus_tag="PLK64_14720"
     CDS             320..667
                     /locus_tag="PLK64_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009071874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HPV81045.1"
                     /translation="MSQIPPFPQDSATPAQQPSALVCPKCRGTMRTYDRNGIHVEQCE
                     TCRGLFLDFGEFEALARLEANVHAAAAAPPAPPPQPAPGYQTGYPSYGPEWGYRGGHH
                     YRKRGLSGLFFSS"
     gene            complement(717..1742)
                     /locus_tag="PLK64_14725"
     CDS             complement(717..1742)
                     /locus_tag="PLK64_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPV81046.1"
                     /translation="MSDVLGVLDPAAAPSLRWGIIAPGGIANKFAEAVSQFTAGQVVA
                     VSSRDEGRARSFAERHGIPRAYAGHDALGADPEVDAVYVASPHSAHREHAVAMARAGK
                     HVLIEKALARNLVEVNEIFDSARGAGVFAMEAMWTRHLPHIAAVRRWIADGRIGEVVS
                     VVADHGQSLDLGPDHRLKAPELAGGALLDLGVYPVAFVVDLLGAPTSVHAAGHRTETG
                     VDASVALTLGYPDPVFAQASCTLKVKTPTTAVVCGTGGRIELDGDFYGGTVARLLEPG
                     KVREVVEEFDGRVANGFQFEIAEVARCVADGQLESERMRWDDSRAVMSVLDDARRQMG
                     VRYPGEA"
     gene            complement(1739..2188)
                     /locus_tag="PLK64_14730"
     CDS             complement(1739..2188)
                     /locus_tag="PLK64_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPV81047.1"
                     /translation="MTSTDPSLGLRSVRLERVAAGRYAVTNPRGGTVTLGRGQDSDYT
                     PVELLLAAIAGCTSVDVDEVTTRRSEPESFVVDCSGVKVSDNLGNHLSEVEVTFRISF
                     PDTESGAAAAALVPRLVSLSHEKLCTVSRTVMLPTPVRTVIADEGQE"
     gene            2234..2896
                     /locus_tag="PLK64_14735"
     CDS             2234..2896
                     /locus_tag="PLK64_14735"
                     /inference="COORDINATES: protein motif:HMM:NF025198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HPV81048.1"
                     /translation="MSTDPTASEPSPLDPTAPLSSGYDPAATTPMSPPPPVTPPTAPA
                     GPWDSTPAAPPGPVAPPVAAAPPVPDYGQPAAYGQPAPYGQQAPYGQQAPYGQQPPYG
                     QQPPYGQQPGYGQPSPYAAPPAYGGAAYAGIEHPQGTLILILGILSIVFCGLTGPFAW
                     VMGRKAMREIDTSGQPYSNRGQVQAGMITGIIGSVILVLGVLYLVFVFFIVGMAASSS
                     IR"
     gene            complement(2976..>3818)
                     /locus_tag="PLK64_14740"
     CDS             complement(2976..>3818)
                     /locus_tag="PLK64_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPV81049.1"
                     /translation="PAPDYPLWTAATALAGGTPVHYLCDESEGWVPDLADMAAKITPR
                     TKAIVVINPNNPTGAVYPDHVLRGIIALAREHELVLMADEIYDKILYDDATHTALASL
                     APDVFCLNFNGLSKAYRVAGFRSGWLALSGPKHHARSFIQGLNILANMRLCANVPAQH
                     AIATALGGYQSITELILPGGRLLAQRNLAWELINSIPGVSCTKPGGALYLFPRLDPEV
                     HEIHDDERFALDLVRQAKILVVQGTGFNWPRPDHFRIVTLPHVQELEEAIGRIGNFLA
                     SYRQ"
BASE COUNT          576 a         1370 c         1308 g          564 t
ORIGIN      
        1 cctcgacgat ctcgggtggg gcgacccggc gtgggacctg gcccgaccgg ctgggttctg
       61 ggctgctggg ctgctgtcga cggacgactg ggagggtttc ctcgcggcat accgtgctgc
      121 cggcggtccc ggcgtgccgg tgacgggcga cccgtggccg gcgctcgacc tgcctgcgcg
      181 agcggcagtc gtcatcgccg cgacccgcgc ggtcggcgct gtcggggctg tcggcgcgag
      241 gcggtcagga gctgacacgc acagcgaccc cacagcggag gcactgctgg atgcctgccg
      301 gaagatgtga actctcgcca tgagccagat cccaccgttc ccgcaggact ccgccacccc
      361 cgcccagcag ccgagcgccc tcgtgtgccc caagtgtcgc gggacgatgc ggacctacga
      421 ccgcaacgga attcacgtcg agcagtgcga gacctgtcgg ggcctcttcc tcgactttgg
      481 cgagttcgag gcgctcgcgc ggctggaagc caacgtgcac gcggctgccg cagcgccgcc
      541 cgcccctccc ccgcagcctg cgccgggcta tcagaccggt tacccgagtt atggcccgga
      601 gtggggctac cgcggtggcc accactaccg caagcgcggc ctgtccggcc tcttcttctc
      661 cagctaaccc cccgcacccc ctgcccccct tgccgggggc gggggcaaga cagaaatcag
      721 gcctcgccgg ggtaacgcac ccccatctgg cggcgagcgt cgtcgagcac gctcatgacg
      781 gcgcgcgagt catcccagcg catccgctcg ctctcgagct gcccgtccgc cacgcaccgc
      841 gcgacctcgg cgatctcgaa ctggaacccg ttggccaccc gaccgtcgaa ctcctcgacg
      901 acctcgcgga ccttgcctgg ctcaagcagt cgagccaccg tcccgccata gaagtcgcca
      961 tcgagctcga tccgcccgcc ggtcccacag accacggccg tcgtcggcgt cttgaccttg
     1021 agcgtgcacg acgcctgcgc gaacaccggg tcggggtagc ccaacgtcag cgccacactc
     1081 gcgtcgaccc ccgtctcggt gcggtgcccg gccgcatgaa cgctcgtcgg cgcaccgagc
     1141 aggtccacca cgaaggccac cggatagacc ccgaggtcaa gcagcgcgcc gccggccaac
     1201 tcgggcgcct tgagccggtg gtccggcccg aggtccaggc tctgaccgtg gtcggcgacg
     1261 acggagacga cctcgccgat cctcccgtca gcgatccagc gacgcacggc ggcgatgtgc
     1321 ggcaggtgcc gggtccacat cgcctccatc gcaaacacgc ccgcgccgcg cgccgagtcg
     1381 aagatctcgt tgacctcaac gaggttgcgc gccaacgcct tttcgatgag gacgtgcttg
     1441 ccggcccgcg ccatggcgac cgcgtgttcg cggtgggccg agtgcggcga cgcgacatag
     1501 accgcgtcga cctcggggtc ggcgccgagg gcgtcgtgcc cggcatacgc cctcggtatg
     1561 ccgtgccgct ccgcgaacga gcgcgcccga ccctcatcgc gtgaggacac cgcgacgacc
     1621 tgcccggcgg tgaactggct gaccgcctcg gcgaacttgt tggcgatgcc acccggcgcg
     1681 atgatgcccc agcgcaggct cggtgcagca gcggggtcca agacgcccag tacgtcgctc
     1741 attcctggcc ctcgtcggcg atgacggtgc gcaccggggt gggcagcatg accgtccggc
     1801 tgaccgtgca cagcttttcg tgcgagagcg agaccagccg agggacgagc gccgcggcag
     1861 ccgccccgga ctcggtgtcc gggaaggaga ttcggaaggt cacctcgacc tccgacaggt
     1921 ggttgccgag gttgtcggag accttgaccc cactgcagtc cacgacgaag gactccggct
     1981 cgctgcgccg ggtggtcacc tcatcgacgt caacgctcgt gcagccggcg atcgctgcga
     2041 gcaacaactc caccggcgtg tagtcggagt cttggccacg gccgagcgtg accgtgccgc
     2101 cgcgggggtt ggtcacggca taccggccgg cggcgacgcg ctccaagcgg accgaacgca
     2161 ggccaaggga agggtcagtc gaggtcatgg ggacagcctg ccacgggcaa cgggggcaag
     2221 tagggtggtg ttcatgagca ctgatccgac ggcgagcgag cccagcccgc tcgaccccac
     2281 ggcccccctc tcctccggat acgatccggc ggcgaccacg ccgatgtccc cgcccccgcc
     2341 ggtgacgccg cccacggcac ccgccggtcc ctgggacagc accccggccg cgccccctgg
     2401 ccccgtcgcc ccgcccgttg ccgcagcccc gccggttccc gactacgggc agcccgccgc
     2461 ctacggccaa cccgcccctt acggccagca ggccccctac gggcaacagg ctccgtacgg
     2521 ccagcagcct ccctacgggc aacagccgcc gtatggccag cagccgggtt acggccagcc
     2581 ctccccgtat gccgcgccgc ccgcctatgg cggtgctgcc tacgccggca tcgagcaccc
     2641 gcagggcacc ttgatcctca tcctcggcat cctctcgatc gtcttctgcg gtctcaccgg
     2701 acccttcgcc tgggtgatgg gtcgcaaggc catgcgggag atcgacacca gcggccagcc
     2761 ctacagcaac cgcggccagg tccaggccgg catgatcacc ggcatcattg gctcagtcat
     2821 cctcgtcctc ggagtcctct acctcgtttt cgttttcttc atcgtcggga tggcggccag
     2881 cagcagcatt cgctgaccca ccctcaaccc cgcgaacgtg agggggcggc ataccgagtc
     2941 ggtatgccgc cccctcacgt tggtgcgcga atcggctact gccggtagct ggccaggaag
     3001 ttgccgatgc gtccgatggc ctcttcgagc tcttggacgt gtggcagcgt gacgatccgg
     3061 aagtgatcgg gccgcggcca gttgaagccg gtgccctgca cgacgaggat cttggcctgg
     3121 cggaccagat cgagggcgaa gcgctcgtcg tcgtggatct cgtggacctc ggggtcgagc
     3181 cgggggaaca ggtagagggc gccgccgggc ttggtgcacg agacgccggg gatcgagttg
     3241 atgagctccc acgcgaggtt gcgctgggcg agcaggcggc cgccggggag gatcagctcg
     3301 gtgatcgact ggtagccgcc gagcgccgtg gcgatcgcat gctgcgcagg cacattggcg
     3361 catagccgca tgttcgccag gatgttgagg ccctggatga aggaccgggc gtggtgcttg
     3421 gggcccgaga gcgccagcca gccggaccgg aaaccggcga cccgataggc cttggacagc
     3481 ccgttgaagt tgaggcagaa gacgtcgggc gccagggacg ccagggccgt gtgggttgcg
     3541 tcgtcgtaga ggatcttgtc gtagatctcg tcggccatga ggacgagttc gtgctcgcgg
     3601 gccagggcga tgatgccgcg caggacatgg tcgggataga ccgcgccggt ggggttattg
     3661 gggttgatga cgacgatcgc cttggtgcgc ggggtgatct tggccgccat gtcggccagg
     3721 tcggggaccc acccctcgct ctcgtcgcac aggtagtgca ccggggtgcc accagcgagc
     3781 gcggtcgcgg cggtccacag cgggtagtcc ggcgcggg
//
LOCUS       DAOMZY010000331         3803 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_22932_length_3803_cov_5.432231, whole genome shotgun sequence.
ACCESSION   DAOMZY010000331 DAOMZY010000000
VERSION     DAOMZY010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22932_length_3803_cov_5.432231"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="PLK64_14745"
     CDS             <1..1134
                     /locus_tag="PLK64_14745"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV81050.1"
                     /translation="LAAEMAVKIRAQEPAYRDGGMVSGDELQRVCRGNLHLVLGELAG
                     LHEFDPTAVRAVGRQRAEQGLPIDVVLHAFRVGTRHLWEALVERADDEIRAELLLSAA
                     DIWAVSDRLALEVTDAYRAAESARTQLDRQVREAVLDALLLGDGEQSRMWDSAQLLRM
                     PQEGRFVVIAAECPGPGQEAVPSAESRLKERNVSSAWRLDAGLQEGVVALQPRFGEQA
                     LANALAAGARGRVGVSRPYRHLDETSQALVEARLAFQTVPPRTIGVASFGHHPLDVFI
                     VSSPIAAEQLARAVLAPILDLAPAERDPLLDTVRTWLAVAGSTKEAAARLHMHRNGVR
                     YRLNRFEELVGRRLAVPTQLAEIHLALQAVTVLGLSAPPTSSL"
     gene            complement(1445..2356)
                     /locus_tag="PLK64_14750"
     CDS             complement(1445..2356)
                     /locus_tag="PLK64_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPV81051.1"
                     /translation="MSTRPDLRLLELLVALAEAGSLSGAGRAMGMAQPNVSRALSRLE
                     RQLSVQLVTRTPGGSSLTPEGVVVLEWARAALDAVDRVVIGAKSLAAQQDTSLHVAAS
                     LTVAEYLAPSWLSRLRRARPDVRVSLAVSNSDLVLEQVISGAVDLGFVEAPTVPRTVS
                     SRVVARDELIVVVGPTHPWATRLDPVGPNELAATDLVMRESGSGTRQTLERALRVTGV
                     EVGDTHLELASTAAVKSAAATGDTPAVLSQLAVATELGSGSLVRVPVEGLRLGRRLRA
                     VWPAGMPLGGVAAELVRLVAEAPPAHP"
     gene            2453..3577
                     /locus_tag="PLK64_14755"
     CDS             2453..3577
                     /locus_tag="PLK64_14755"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HPV81052.1"
                     /translation="MTNTVQTPPAHKAAPNHVPIDRPMAPEPDPAPAPDPAPAPDPAP
                     VPGRPLGPGRPLIPGLALAAGGGALGLAFHSVVPALSGVLIALATGMAASGALRSRPH
                     LAPGLEFGSRRVLRFGVALLGFQLVAGQALALGWQTLAGVALIVAGGIVGTMLIGDAL
                     GVPTGRRLLIACGFSICGAAAVAAVDGVVESNEEDVATAIGLVVAFGSMAMLLLPSLA
                     TLFGLDARDAGAWMGGSIHEVGQVIVAGGIVGGVALEIAVLVKLTRVLMLGPVLVALT
                     MRARRSQLKVSGAPAPLFPFFVIAFAVLVVLGSVLTVPTPIRSGVSFIQEFSLATAMF
                     ALGAGVDVRGLRKLGAPEVLLGVLSSALVAVLATSLVLVA"
BASE COUNT          566 a         1434 c         1223 g          580 t
ORIGIN      
        1 cttgccgccg agatggcggt gaagattcgc gcgcaggagc cggcctaccg ggatggcggg
       61 atggtctcgg gcgacgaact ccaacgcgtc tgtcgcggga acctgcacct ggtcctgggt
      121 gagcttgccg gcctgcacga gttcgacccc accgcggtcc gggccgttgg ccgccagcgc
      181 gcggagcagg ggttgccgat cgacgttgtc ctgcacgcct tccgcgtcgg cacgcgacac
      241 ctctgggagg cccttgtcga gcgcgctgac gacgagatcc gcgcagagtt gctgctctcg
      301 gccgccgaca tctgggcggt ctctgatcgc ctcgctctgg aagtgaccga cgcctaccgc
      361 gctgccgagt ccgcccgcac ccaactggac cgtcaggtcc gcgaggccgt gctcgatgcg
      421 ttgttgctcg gcgacgggga acagagccgc atgtgggact cggcccaatt gctccgtatg
      481 ccgcaggagg gccggttcgt cgtcatcgct gcggagtgcc ctggcccggg gcaggaggcc
      541 gtgccgtcag ccgagtcccg actcaaggag cgcaacgtct cctcagcctg gcgactcgac
      601 gccggcctgc aggaaggtgt cgtcgccctg caaccccgtt ttggtgagca agccctggcg
      661 aatgccctcg ccgcgggcgc ccgggggcgg gtcggggtga gcaggcccta ccgacacctt
      721 gatgagacca gtcaggccct ggtggaagcc cggctcgcct tccagaccgt tccccctcgg
      781 acgatcggtg tggcctcctt cggccaccac cccttggacg tcttcatcgt cagctcaccg
      841 atcgccgccg agcagttggc ccgcgccgtc ctcgcgccaa tcctcgacct ggcgcccgcc
      901 gaacgggacc cgctgctgga cacggtgcgc acgtggctgg ctgtggccgg ctccaccaag
      961 gaggctgcgg cgcgtttgca tatgcaccgc aacggagttc gctatcggct caaccggttc
     1021 gaggagctcg ttgggcgtcg attggccgtg cccactcagc tcgccgagat ccacctcgcc
     1081 ctacaggccg ttaccgtcct cggcctcagc gctccgccga cgtcgtccct ctgaacctgc
     1141 cgtcacccaa gcccgcgccc gggcaagccg cccacgctca cgccttgcgc ggcatacctc
     1201 gcgcggagcg ggtatgccgc ccgctctcgg cttgcgcgtg cgtcggagag tgcgtttggc
     1261 ggcgcgataa ccccctcgat cgcactctgt cgcgcccaca cccctgagcc acggcacaag
     1321 tgaccatgcg agcgctatct ccgggccgaa acgccggggt ctgcccccaa agatgaccat
     1381 gcgagcgcta tctccgggcc gaaaccccgg ggtctgcccc caaagatgac catgcgagcg
     1441 ttatctacgg gtgcgctggc ggcgcctcgg cgacgaggcg gaccagttcg gcagcaactc
     1501 cgcccagcgg catacccgcc ggccacaccg cccgcaaccg gcgacccagc cgaagcccct
     1561 caaccggtac tcggaccaac gacccactgc cgagctcggt ggcgaccgca agctggctga
     1621 ggaccgctgg ggtgtcgccg gtggcggcag ccgacttcac agccgcggtg ctggccaact
     1681 cgagatgggt gtcacccacc tcgaccccgg tgacgcggag agcccgctca agggtctgcc
     1741 gggtgcccga cccgctctcg cgcatcacca ggtcggtggc ggccaactcg ttcggcccaa
     1801 cgggatcaag gcgcgtagcc caagggtggg ttgggcccac gaccacaatc aactcgtcgc
     1861 gggccacgac acgggacgag acggtccgcg gcacagtagg ggcttcgacg aagcccaggt
     1921 cgaccgcacc actgatcacc tgctccagca ccagatccga gttggacacc gccaggctta
     1981 cccgaacgtc gggtcgggcc cgccggagcc gagagagcca cgagggagcc agatactcgg
     2041 cgacggtgag actggccgcg acatgcaggc tcgtgtcctg ctgcgcagca aggctttttg
     2101 cgccgatgac cacacggtcg acggcatcga gcgcagcccg agcccactca agaacgacca
     2161 caccttcagg cgtgagcgac gaaccgccgg gagtccgcgt gaccaactgg acggacaact
     2221 gccgctccaa acgcgacagc gctcggctga cgttcggctg ggccatgccc atcgcccgcc
     2281 ctgcaccact cagcgagccc gcctccgcca acgcaacgag caactcgagc aagcggaggt
     2341 ccggacgcgt cgacacccaa gcacagtagc gcggcagggt atatcaccag cgcataaggt
     2401 caggtcgaag tggtctctag cggcgccgtc gcggctcggc gacgctgacg atgtgaccaa
     2461 caccgtccag actccccccg cccacaaggc ggcaccaaac cacgtgccga tcgaccgacc
     2521 catggcgccc gagccagacc cagcgcccgc cccagaccca gcacccgccc cagacccagc
     2581 acccgtccca ggccggcccc tcggcccagg ccggcccctc attccaggcc tggcgctcgc
     2641 cgccggcggt ggcgcgctcg gccttgcctt ccacagcgtc gtcccggccc tgagcggagt
     2701 cctcatcgcc ctcgccaccg gcatggcggc gtccggcgcc ctgcgctcac ggccgcacct
     2761 cgctcccggt ctggagttcg gatcgcggag ggtcctgcga ttcggggtgg ccctgctcgg
     2821 gttccagttg gtcgccggac aggcgctcgc cctcggctgg caaaccctgg cgggcgtcgc
     2881 gctcatcgtg gccgggggga tcgttgggac catgctgatc ggcgacgctc tcggggtccc
     2941 gaccgggcga cgcctgctca tcgcctgcgg cttctcgatc tgcggcgcgg cagccgtggc
     3001 agcggtcgac ggcgtcgtcg agtcgaacga ggaggacgtc gccaccgcca ttggcctcgt
     3061 cgtcgccttc ggcagcatgg cgatgctcct gctgccttct ctggcaacct tgttcggcct
     3121 cgacgcgcgt gatgctggcg cctggatggg cggcagcatc cacgaagtcg gccaagtgat
     3181 cgtggccggc ggcatcgtcg gcggcgtcgc ccttgagatc gccgtcctcg tcaagttgac
     3241 ccgtgtcctc atgctcgggc cggtgctcgt cgcgctcacc atgcgggccc gccgcagtca
     3301 gctcaaggtc tcaggcgcgc cggctcccct cttccccttc ttcgtgatcg cgttcgccgt
     3361 tctggtcgtc ctcggtagcg tcctcaccgt ccccacaccc atccggtcag gggtgagctt
     3421 catccaagag ttctcgttgg ccacggcgat gttcgccctc ggtgccggcg tcgacgtgcg
     3481 cgggctgcgc aaactcggcg ccccggaggt gctcctcggc gttctctcct ccgcgctcgt
     3541 cgcggtgctc gccacctcgc tcgtcttggt cgcctagccg gccacgcggc atacccgccg
     3601 cggccgcgca cggagagcac gcggcatacc gaacagctca gtcccagccc cgctcctcgc
     3661 ggccagaggt atgccgcccg cacccccccg cccgcaaaag atgaccatgc gagcgctatc
     3721 tccgggccga aaccccgggg tctgccccaa agatgaccat gcgagcgcta tctccggccc
     3781 cgagctaggt gggtcgccac tcg
//
LOCUS       DAOMZY010000332         3799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_22964_length_3799_cov_5.818643, whole genome shotgun sequence.
ACCESSION   DAOMZY010000332 DAOMZY010000000
VERSION     DAOMZY010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22964_length_3799_cov_5.818643"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PLK64_14760"
     CDS             complement(<1..517)
                     /locus_tag="PLK64_14760"
                     /inference="COORDINATES: protein motif:HMM:NF014712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HPV81053.1"
                     /translation="MPSETEPGLVRVLPCGETALLIEVADTLTAVALARQFDHLRAAG
                     GPPWSDVHDVVTGERTVLVIAGHVTTMTELRPAILESVSAARLPVRELGTGGGQTPEI
                     VAPLEIPVVYDGPDLLDVAALTGLSPAQVVDAHTGAEWDVGFSGFAPGFAYLVGGDPR
                     LVVPRRAEPRPR"
     gene            complement(501..1277)
                     /locus_tag="PLK64_14765"
     CDS             complement(501..1277)
                     /locus_tag="PLK64_14765"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HPV81054.1"
                     /translation="MSDNRVVHIDLNADLGESFGAWTMGDDLALLDVVTSANIACGFH
                     AGDPLTMTRTVGAALDRGVTVGAHVSYRDLVGFGRRFIDASEEEVRADVLVQLAALDG
                     VCRAAGGSVTYLKPHGALYHAAARHEPHARAIVAAVAAYDGSITVLGMAGSRLLALAA
                     EAELPTATEAFADRGYLADGTLVPRGEPGDLLHDPDEIAARVVGMARDGAVPSVEGTA
                     VPISADSVCVHGDTPGAVAIARAVRAALEQAGVAVRAFGD"
     gene            1470..3716
                     /locus_tag="PLK64_14770"
     CDS             1470..3716
                     /locus_tag="PLK64_14770"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPV81055.1"
                     /translation="MSASLAARRWTHRAFGGAVALALAGSAAMTAQAATGSAPIDPNS
                     DGVVATSGVDSSSALIQLVGDPLSTSPDVDHGNGRVKLDGQKTKSVKAALAAQRNALK
                     SWLRANAPKAKVTGEYDFALNAIAVRLNGTSVATLRQAPGVASVQLQGLFHPTADDPD
                     LARIDGLLGWSVAGATSVPSDPSSWAGAGVQVGIIDSGVDASHPCFSDERFPATRQLG
                     DTHFTNNKVIVAKVFNNKAGSKGYTAEAIGEHGTHVGGTVACNLQTPAVVNGVDITYD
                     PSGVAPGAQLGSYNVFPGDDGDARSEDILNAIEAAVADGMDVLNMSLGGGSHGIQDLL
                     TVAVDNVDRAGIVVAVSAGNEGPGVMTIGSPGMAERALTSGASTVGHFVGLSIKGEGT
                     EVYAATGDFPAPTAPISATLEAVKNADGTLGNGCAAGAVSAAVPAGSIALISRGTCTF
                     GTKVANAKAAGAVAAIIVNNVAGDPIAMAADTALPADVQQLPAVMTALSTKGALLALD
                     GQPVTIGSDQAYRVTTSNDIMAGFSSQGPVDVSYRVKPDVVAPGVNVLSSIPMSYCDN
                     DVEGCWAFFQGTSMSSPHLAGMAAVVRSANPGWDAWQVRSAIANTAKLGVLTSFTDGT
                     TVDVNVQKVGNGLADLDAAVLAQVALTRPSVSFGAVPNTAGQSLTETVTVTNLTDGAL
                     TLPLSVSDGPGEGAFTVSPASVNLPAGGSATVTVTFTSAKGSALGNTQATLMVGSAAH
                     AALYAFLK"
BASE COUNT          581 a         1360 c         1318 g          540 t
ORIGIN      
        1 cgcgcgggcg aggctcggcc cggcgcggga cgacgagcct cgggtcgccg ccgaccagat
       61 aggcgaagcc gggggcgaac ccggagaacc ccacgtccca ctcggccccc gtgtgcgcgt
      121 cgaccacctg tgcggggctc agcccggtga gggctgccac gtcgagcaga tccggcccgt
      181 cgtagacgac ggggatctcg aggggggcga cgatctcggg ggtttggccg ccgccggtgc
      241 cgagctcgcg cacgggaagc ctggctgcac tcacggattc gaggatcgcc ggtcgcagct
      301 cggtcatcgt ggtcacgtgc ccggcgatga cgaggacggt gcgctcgccg gtgaccacgt
      361 cgtggacgtc gctccaaggc ggcccgccgg cagcccgtaa gtgatcgaac tgtcgggcca
      421 gggccaccgc ggtcagcgtg tcggcgacct cgatgagcag cgccgtctcg ccacacggca
      481 ggacgcggac gaggcccggc tcagtctccg aaggcacgaa cggccacccc ggcctgctcc
      541 agggcagcgc ggaccgcgcg tgcgatggca acggccccgg gagtgtcacc gtggacgcac
      601 accgagtcgg ccgagatcgg cacggcggtt ccctcgaccg agggcacggc gccgtcgcga
      661 gccatgccga cgacgcgggc agcgatctcg tcggggtcat ggagcaggtc gcccggctca
      721 ccgcgaggca cgagcgtgcc gtcggccagg tagccccggt cggcgaacgc ctcagtcgcc
      781 gtgggcagct cggcctcggc ggcgagcgcc agcaggcggc tgcccgccat cccgaggacg
      841 gtgatcgagc cgtcgtatgc cgcgaccgca gcgacgatcg cgcgcgcgtg cggctcgtgt
      901 cgggcggcag cgtggtagag cgcgccgtgg ggcttgaggt aggtgacgct cccaccggct
      961 gcacggcata ccccgtcgag ggccgcgagc tggacgagga cgtcggcgcg gacctcctcc
     1021 tcgctggcgt cgatgaagcg gcgaccgaag ccgaccaggt cgcggtagga cacgtgggcg
     1081 cccacggtga cgccccggtc gagggccgcc ccgaccgtcc gagtcatggt gagggggtcg
     1141 ccggcatgga agccgcaggc gatgttggca ctggtgacga cgtccaacaa ggccaggtcg
     1201 tcgcccatcg tccaggcgcc gaaggactcg cccaggtcgg cgttgaggtc gatgtgcacg
     1261 acacgattgt ccgacatgga ccggtgtggg gcagcggcct cgcgaccgac cggcccttgg
     1321 ccgcaggatt cgttgctcag gtgcctgttg accggagctt atgcgggcta gcattccccc
     1381 agcggctcgg ggcgcgcgct gcatgcttct gcgcgtggcc ccggtgggag cggacgctcg
     1441 acccggactc aaggacgaga ggatccctcg tgagtgcatc actggctgcg cgtcgctgga
     1501 cgcatcgagc gttcggaggc gcggtcgccc tggccctggc ggggagtgcg gcgatgaccg
     1561 cgcaggccgc gaccggctcg gccccgatcg acccgaactc agacggcgtc gtcgcgacct
     1621 caggggtgga ctcgtccagc gccctgatcc aactcgtcgg cgacccgctc tcgacctcac
     1681 ccgatgtcga ccacggcaac ggccgggtca agctcgacgg gcagaagacc aagagcgtca
     1741 aggcggcgct cgccgcccag cgcaacgccc tcaagtcgtg gctgcgcgcc aacgccccca
     1801 aggcgaaggt gaccggcgaa tacgactttg ccctgaacgc gatcgccgtg cgtctcaatg
     1861 gcacgtcggt cgccacgctg cgtcaggcgc ccggcgtggc ctcggtgcag ttgcaggggc
     1921 tcttccaccc gacggcggac gacccggacc tggctcgaat cgacggcctg ctcggttggt
     1981 cggttgccgg cgccacctcg gtgcccagtg acccgtcatc gtgggccggc gcgggcgtgc
     2041 aggtcggcat catcgactcc ggtgtcgatg ccagccaccc ttgtttcagt gatgaacgtt
     2101 tccctgccac ccggcagctc ggcgacacgc acttcaccaa caacaaggtc atcgtcgcga
     2161 aggtcttcaa caacaaggcc ggcagcaagg gatacaccgc tgaggcgatc ggcgagcacg
     2221 gcacccacgt cggtggcacg gtcgcctgca acctgcagac cccggccgtc gtcaacggcg
     2281 tcgacatcac ctatgacccc agcggcgtgg cccccggagc ccagctcggc agctacaacg
     2341 tcttccccgg tgacgatggt gacgctcgca gcgaggacat cctcaacgcc atcgaggccg
     2401 cggtcgctga cgggatggac gtgctcaaca tgtccctcgg tggcggctcg cacggcatcc
     2461 aggacctgct gaccgtcgct gtcgacaacg tcgaccgcgc gggcatcgtt gtcgccgtct
     2521 cggcgggcaa cgaaggcccc ggcgtcatga ccatcggctc cccaggcatg gccgagcgcg
     2581 cgctcacctc aggcgcctcg accgtcgggc acttcgtcgg cctctccatc aagggcgaag
     2641 gcaccgaggt gtatgccgcg accggcgact tccccgcccc cacggcaccg atctccgcca
     2701 cgctggaggc ggtcaagaac gctgatggca cgctcggcaa cgggtgtgcg gcaggcgccg
     2761 tctccgcagc cgtgccggca gggtcgatcg ccctcatctc gcgtggtaca tgcaccttcg
     2821 ggaccaaggt cgccaacgcc aaggcagctg gtgcagtcgc ggcgatcatc gtcaacaacg
     2881 tcgccggtga cccgatcgcc atggccgccg acaccgccct gccggccgac gtacagcaac
     2941 tgcctgccgt catgacggcc ctgtcgacca agggcgccct gctggcgctg gatggccagc
     3001 cggtgacgat cggttccgac caggcctacc gggtcacgac cagcaacgac atcatggccg
     3061 ggttctccag ccagggtccg gtcgacgtga gctaccgcgt caagccggac gtcgtcgccc
     3121 ccggcgtcaa cgtcctgtcc tcgatcccga tgtcctactg cgacaacgac gtcgagggct
     3181 gctgggcgtt cttccagggc acgtcgatgt ccagcccgca cctggccgga atggctgccg
     3241 tcgtccgctc ggcgaacccc ggttgggatg cctggcaggt ccgctcggcg atcgccaaca
     3301 ccgccaagct gggcgtgctg acgagcttca ccgatggcac cacggtggac gtcaacgtcc
     3361 agaaggtcgg caacggcctg gctgacctcg acgccgccgt cctggcgcag gtcgctctca
     3421 cgcggcccag cgtgtccttc ggtgcggtgc ccaacacggc cggccagtcg ctgaccgaga
     3481 cggtgaccgt gacgaacctg accgatgggg cgctgaccct gccgctgtcg gtctcagacg
     3541 gcccgggcga aggcgccttc acggtctcgc ccgcgtcggt gaacctgccc gctggtggca
     3601 gcgccacggt gacggtgacg ttcacgtcgg ctaagggttc ggcgctcggc aacacccagg
     3661 cgacgttgat ggtcggctcg gcagcgcacg ccgcgctgta cgccttcctc aagtagttgc
     3721 tcgcagcgtt acctcagacg cccccgcccg gacctggtcc aggcgggggc gtctgccgtt
     3781 gcccccgaca ccctcgaca
//
LOCUS       DAOMZY010000333         3796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_22991_length_3796_cov_6.335204, whole genome shotgun sequence.
ACCESSION   DAOMZY010000333 DAOMZY010000000
VERSION     DAOMZY010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22991_length_3796_cov_6.335204"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..1184)
                     /locus_tag="PLK64_14775"
     CDS             complement(234..1184)
                     /locus_tag="PLK64_14775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPV81056.1"
                     /translation="MTRAEVTRAQVTRPQVTRAHAITTVGLVHIYRSEGHDVAALSGV
                     DLSVRAGEVVGLLGPSGSGKSTLLALLGGLFRPSAGKIFVGDRELSALTAAELDDFQA
                     RDTSLMLQGARRNFVPYLTVRENVEFAQAQARRLGAQVDPVERTLGLVGASDFADARP
                     GRLSQGQAQMAALAVAMAPSPGVLLADEPTSALDRRAREVIIEALLRINAEQGTTVVV
                     VTHDPEVAARLPRTVTIRDGRIGGEGRSGQEYAVVTADGFVPLPVHLREELPPGTLLR
                     IERVENGYLLAPEVLGHEVLGHEVLGHEVSGHESDGATGA"
     gene            complement(1181..>3796)
                     /locus_tag="PLK64_14780"
     CDS             complement(1181..>3796)
                     /locus_tag="PLK64_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81057.1"
                     /translation="SPRTTLHVVGIYEQVDGSYWFGERLTGQASARLGFDTMLTPLTT
                     LTERVTAVDGQLTTWFERHYALDLPLLTDTVGVDQIGPLGATVADLVRYPMGVERSGT
                     HVADTVTARSGLPAIAEQVRVGSAQAGITVPLLMAQASLLLVAVLWLVLVAAADQRRS
                     EVAVARLRGRGSSGARRILLAETLPPIILGAPLGAALAAGAVSAARHTLLPSQPPFEV
                     PVGAVVALAAALALMLTLAVVSVRRVCREPVAALLRTVPPRRGSVRLGVLEAMLVAAA
                     AAAFVALVTGSVGGAVGQIAPTLLALAIGVIAARVLSAVLAAGGRRLLLAGRSTSGAA
                     LLTASRRGTTRWLVPVVTIALCIVVVSADSLAVGARNWSDRAGAEVGAATVLTLDSAD
                     LSAVAAAVRAVDPTGEHVTPVALLQPAAEGGTTTVGVVPGAFRRIALWPGVDVAALPW
                     DRLTAPTAPPLVATGSRISYHVTAPALTVVGPAIRPAPTSLALALRVVRADGSVESVP
                     LGTIPATGIDADQDVAVGCSEGCRISGIGVLGPPGAAGVSGTVAVSELAVDGRPFDLG
                     GAGSWHAPAPVDPGGTPSGGATVSGSFAPGSTTLTYDTNGADKAFLAHASRPEVVVAL
                     TTPTSTPSATAATFGGSYVDGSSLLLTSAGGVAFVPGGPAAASIVNLDNLLAQGWRGR
                     GSATLTAYLDTADPDAVARVTTALQANGIHVVATRHAAAVAAAYGETAAAWSLKLALA
                     VGVLAILVAGVGIVVLASTARRARRRDYAALALVGQRPRSLALLAQLETVPVIVASAL
                     IGAAVGWWAAPVAVSMMPLFPAASATFPLDLRTAVAPALVAGLGGLVALTLVGVATSH
                     AAARRADLQQLREAG"
BASE COUNT          627 a         1347 c         1370 g          452 t
ORIGIN      
        1 ccgagtcacg agtcgcttcg ctgctgtcca taaccccgac gctaacagcg accactgaca
       61 ggcaataggt ctcaaacagc ctcttccaca ggcaaacaca tcacagaatg ataacgatct
      121 gacggtcggc tgtcgtcgtc agccgtcgcc gtctgccgca gtcggctggt cagtcgggcg
      181 agtcgtccca cctcaccggc gcccaacggc gcggccgacc gcgtcggcac gccctaagcg
      241 cctgtcgcac cgtccgactc gtgacccgaa acctcatgac ccaaaacctc atgacccaag
      301 acctcatgac ccaagacctc gggagctaac agatagccgt tctcgacccg ctcgatgcgc
      361 aacagcgtgc ccggcggcaa ctcctcccgc aggtgcaccg gaagggggac gaacccgtcg
      421 gccgtgacga cggcatactc ctggccgctg cggccctcac ccccgatccg tccgtcgcgg
      481 atcgtcaccg tgcgcgggag gcgggcggcg acctcggggt cgtgggtgac gacaacgaca
      541 gtcgtgccct gctccgcgtt gatccgcaat aacgcctcga tgatgacctc gcgagcgcgc
      601 cggtccagtg cgctggtcgg ctcgtcggcc aacagcaccc caggcgacgg tgccatcgcc
      661 acggcaagcg ccgccatctg cgcctgtccc tgcgacaagc gacccgggcg cgcgtcggcg
      721 aagtcgctgg ccccgaccag ccccagggtc cgctcgaccg ggtcgacctg cgccccgagc
      781 cggcgggcct gcgcctgcgc gaactcgacg ttctcacgca ccgtcagata gggcacgaag
      841 ttgcgtcgcg ccccttggag catgagcgac gtatccctcg cctggaagtc atccaattcg
      901 gctgcggtga gcgcggatag ctcgcggtca ccaacgaaga tcttgcctgc gctcggtcgg
      961 aagagaccgc cgagcagcgc aagcaacgtc gacttccccg accccgacgg accgagcagc
     1021 ccgaccactt cgcccgcgcg gacactgagg tcgaccccag acagggcagc gacatcgtgc
     1081 ccctcagacc ggtagatgtg gaccagcccc acagtcgtga tcgcgtgggc gcgggtgacc
     1141 tgggggcggg tgacctgggc gcgggtgacc tcggcgcgag tcatccggcc tcccgcaact
     1201 gctgcagatc ggcccggcgc gcggccgcgt ggcttgtggc gacgccgacg agagtgaggg
     1261 cgaccaggcc gccgagaccg gcgacgagcg cgggcgccac cgccgtgcgc aggtcgaggg
     1321 ggaaggtcgc cgacgcggcc gggaagagcg gcatcatcga cacggccacc ggggccgccc
     1381 accacccgac ggcggcgccg atcagcgctg acgcgacgat gacgggcacg gtctcgagct
     1441 gggccagcag cgcgaggctg cgggggcgtt gcccgacgag ggccagcgcg gcatagtcac
     1501 gtctgcgggc gcgacgggca gtcgaggcga ggacgacgat ccccaccccg gcgacaagga
     1561 tcgccaggac acccaccgca agggccagtt tgagactcca cgcggcggcg gtctccccgt
     1621 atgccgcggc caccgccgcc gcatgccgcg tcgccaccac gtgaatcccg ttggcctgca
     1681 aggccgttgt cactcgcgcg accgcgtcgg ggtcggccgt gtcgaggtat gccgtgagcg
     1741 tggcggagcc gcgcccgcgc caaccttggg caagcaggtt gtcgaggttg acgatgctcg
     1801 ctgcggcggg gccgccgggg acgaatgcca ccccgccagc ggacgtgagc aacagcgagc
     1861 ttccgtcgac gtaggaccca ccgaaggtgg cggcagtggc gctcggtgtg gaggtcgggg
     1921 tggtcagcgc gaccacgacc tcgggccgcg aggcgtgagc gaggaacgcc ttgtcggcgc
     1981 cgttggtgtc ataggtcagc gtggtcgatc cgggcgcgaa actcccgctg accgtggctc
     2041 cgcccgaggg agttccgccc gggtctacgg gcgccggtgc gtgccacgag cctgcgccgc
     2101 cgaggtcgaa cgggcgcccg tcgacggcca actcgctcac cgccacggtg ccggaaacgc
     2161 ccgccgctcc aggtgggccc aggaccccga tcccgctgat ccgacagccc tccgagcagc
     2221 cgacggcaac atcctgatcg gcatcaatgc cagtcgcggg gatcgtcccg agggggaccg
     2281 actcgaccga cccgtcggct cggacgacgc gcagggccag ggccaacgag gtcggggctg
     2341 gccggatcgc gggcccgacg accgtcaagg ccggagcggt gacgtgatag gagatccggc
     2401 tcccggtggc aaccaacggg ggagcggtcg gggccgtgag ccggtcccac ggcagggcgg
     2461 cgacgtcgac tccgggccac agggcgatcc gacggaatgc ccccgggaca acgccgacgg
     2521 tggtcgtgcc gccctccgcg gccggctgaa ggagagcaac cggcgtgacg tgctcgcccg
     2581 tcgggtcgac ggcgcgcact gctgcggcga cggcggacag gtcggcggag tcgagggtca
     2641 gcacggtggc ggcacccacc tctgcgccgg cccgatcgct ccagttgcga gccccgacgg
     2701 ccagcgaatc cgcgctgacg acaacgatgc acagcgcaat ggtgacgaca gggacgagcc
     2761 accgagtggt cccgcggcgg ctggcggtca gcaaggcagc acctgacgtc gaccgtccgg
     2821 cgagcaggag tcgccggccc ccggcggcga ggacggccga caggacgcgc gccgcgatca
     2881 ccccgatagc aagcgcgaga agggtcggcg cgatctgccc gaccgcgccg ccgaccgatc
     2941 cggtaaccaa ggcgacgaac gccgccgcag ccgccgcgac gagcattgct tcgaggacgc
     3001 ccagccgcac gcttccccgc cgcggcggga cggtgcgcag caaggcggcg acgggctcgc
     3061 ggcatactct ccggaccgag acgaccgcga gagtgagcat gagcgcgagc gctgcggcga
     3121 gcgcgacgac cgctccgacc ggcacctcga agggcggctg ggacggaagc aaggtatgcc
     3181 gtgcggccga caccgccccc gccgccaggg ctgctcccag gggagcgccg aggatgatcg
     3241 gtgggagggt ctcggcgagc aggatccgac gagcgccact cgagccgcgg ccgcgcagcc
     3301 gggccacggc gacctcgctg cggcgctggt cggcggcggc gacgaggacc agccagagga
     3361 cagcgacgag cagcaggctc gcctgggcca tgagcagcgg caccgtgatg ccggcctgag
     3421 cgctgccgac gcggacctgc tcggcgatcg cgggcaggcc gctgcgcgcg gtgaccgtgt
     3481 cggcgacatg ggtgccggat cgttcgacgc ccatcgggta gcggacgagg tcggccacgg
     3541 tcgctccgag cgggccgatc tgatcgactc ccacggtgtc ggtgagcagc ggcagatcga
     3601 gggcgtagtg ccgctcgaac cacgtggtca actggccgtc gacggcagtc acccgctcgg
     3661 tgagcgtggt cagcggggtg agcatcgtgt cgaatccgag cctggcagag gcctgcccgg
     3721 tcaaacgctc gccgaaccag tagctcccgt cgacctgttc gtagatgccg acgacgtgga
     3781 gggtggtgcg ggggga
//
LOCUS       DAOMZY010000334         3792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_23021_length_3792_cov_6.123093, whole genome shotgun sequence.
ACCESSION   DAOMZY010000334 DAOMZY010000000
VERSION     DAOMZY010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23021_length_3792_cov_6.123093"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..529)
                     /locus_tag="PLK64_14785"
     CDS             complement(185..529)
                     /locus_tag="PLK64_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HPV81058.1"
                     /translation="MYPTNLAKPLQIRRALGIYRVLAIVAGIALFILVVEMVMKYGMH
                     QENWFTKNWSYIHGFIYMAYAASIANLGLKLAWPMMRIVVNLLSGFVPLAPFFAERKI
                     TAETNGILARVY"
     gene            complement(532..1359)
                     /locus_tag="PLK64_14790"
     CDS             complement(532..1359)
                     /locus_tag="PLK64_14790"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HPV81059.1"
                     /translation="MLRTALTPRWLVWLVVVLGLGAGFVWLGLWQMNVAREDAAAEVL
                     REAAARPVLELTATIQPHAAFPADGSNQRMQAVGRFEPARQVLVADRRLAGADGFWVV
                     QPFVVQETGARIPVVRGFVRSLAAADAVAANSAAGSGATEPLTLVGSLAPTESSSAGK
                     KRLPDGQIGSIDVGALLNDWGGEVYNAFLFDIRETPDQGVTGPTGASASGGMERVPPP
                     DLPSGLTLRNAAYALQWWVFAAFGLWMWVKMVRDDYRRHQPVPPLGAGPTADVGATQ"
     gene            1440..3188
                     /locus_tag="PLK64_14795"
     CDS             1440..3188
                     /locus_tag="PLK64_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPV81060.1"
                     /translation="MRPLPYDHPGTPVLTSPMAFLGWVGRRQWRTLLGGVTFGVIWMV
                     AQALIPATLAKAIDEGIIPGDTGALLRWSGALLGLAAVTALAGAARHQLAVANWLRAA
                     FRSVQLIGWHSADTGEALPRDTPTGDVVATVASDSMRLGGAYDTTARFAGAIVSYIVV
                     AVILLRASMPLGLLVLIGVPVLVGLLSFIVRPLQRRQAQQREESGRLIGLGADTVAGL
                     RVLRGIGGETTFLARYAAQSQQVREVGYRVAGVQAALDAAQVLLPGIFVVLVTWLGAR
                     FAIDGRITPGQLVAFYGYSAFLVTPLRTATEFVDRLTRAHIGARKIIRVLKVEPDHVD
                     HADVVTPGATEPPADSALRDERSGVVVEPGQFLAVVSGRPEESAALADRLGRFGTDVE
                     GVTLGGIPLTHLPLATVRRRILVSETDPRLFTGVLRDELDPWGAHSDEEILAALTIAS
                     GEDVLEALADGLDSEVEERGRSFSGGQRQRLALTRALLANAPILVLVEPTSAVDAHTE
                     ARIADRLATFRAGRTTVVMTASPLVLDRADRVLLLQDDAILAEGTHHELMHRRDGASA
                     AYRAVVVRGADEEETR"
     gene            3188..>3792
                     /locus_tag="PLK64_14800"
     CDS             3188..>3792
                     /locus_tag="PLK64_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HPV81061.1"
                     /translation="MSVSAGVPSARRTLPVATLASVRAQTAALLREHRGALIEVVGLH
                     AIAAVAGLAAPFVVGRLVDEVTAGTAYESVDRQALWLAGAILAQTVLVWAARRSSFRL
                     GERVFAQLREQFMTRVMALPLSTVERAGTGDLVSRTTNDVEALSHVVRFGIPSIFVAV
                     VTLAITVAAAVATGWLVALPVVVGVPIIWLSTRRYLRFAPDG"
BASE COUNT          524 a         1363 c         1317 g          588 t
ORIGIN      
        1 accgacatcg tgtgcgggac gacctcgctg tagaccttcg cctcgcggac gcggcgcgcg
       61 atgagttggg catactgcgc gccgaaatcg acgacaagga ccggccggga ctgcaacgtc
      121 tcactcaccc gccaagccta ccgaggcgct ggccccccgc gtggcggtcg ccgcggtgtc
      181 gctctcagta aacccgagcg aggatgccgt tggtctccgc cgtgatcttg cgctcggcga
      241 agaacggcgc cagcggcacg aacccgctga gtaggttgac gacgatccgc atcatcggcc
      301 aggcgagttt gaggccgaga ttggcgatcg acgcggcata ggccatatag atgaagccgt
      361 ggatgtagga ccagttcttc gtgaaccagt tctcctggtg catcccgtac ttcatgacca
      421 tctcgacgac gaggatgaac aacgcgatgc cggcgacgat ggcgagcacc cggtagatcc
      481 ccagcgcccg gcggatctgc aacggtttgg ccaggttggt cggatacatg ctcactgcgt
      541 ggctcctaca tcggcggtcg gcccagcacc cagcggaggc accggctgat gacgacggta
      601 gtcgtcgcgc accatcttca cccacatcca caggccgaac gccgcaaaga cccaccactg
      661 cagggcgtat gccgcgttgc gcagggtcag cccggacggc aggtcggggg gcggcacccg
      721 ctccatcccg cccgatgccg aggctcccgt gggccccgtc accccttggt ccggcgtctc
      781 gcggatgtcg aagaggaagg cgttgtagac ctcgccgccc cagtcgttga gcagggcgcc
      841 gacgtcgatc gatccgatct gcccgtcggg cagacgcttc ttgccagcgg acgacgactc
      901 ggtgggggcc aggctcccca cgagggtgag cggctcagta gcgcccgaac cagccgcgga
      961 gttcgccgcc acggcgtcgg ccgcagcgag cgatcgaacg aagccgcgca ccacggggat
     1021 ccgcgcgccg gtctcttgca cgacgaacgg ctgcacgacc cagaacccgt cagcgccggc
     1081 cagccgacgg tcggcgacga gcacctggcg cgcgggctcg aaacggccca cggcctgcat
     1141 ccgttggttg gacccatcgg cagggaacgc cgcgtgcggc tggatcgtcg cggtcaactc
     1201 gagcaccggg cgggccgcgg cctcgcgcag gacctccgcg gcggcgtcct cgcgggcgac
     1261 gttcatctgc cacagcccga gccagacgaa cccggcgccc agccccagca ccacgacaag
     1321 ccacaccaac cagcgagggg tgagggcggt ccgcagcacg cctcccaggc tacggcgcgc
     1381 ggcatacccc ggagaaaacg gggtgaagcg gtctggcggc gcgggtagcg tggatccttg
     1441 tgcgccctct gccctacgac catcccggga ccccggtgct caccagcccg atggccttcc
     1501 ttggctgggt gggccggcgg cagtggcgca ccctcctggg cggagtgacc ttcggtgtca
     1561 tctggatggt ggcccaggcg ctgatcccgg cgaccctcgc caaggccatc gacgaaggga
     1621 tcattccggg cgacaccggg gccttgctgc gttggtcggg tgccctcttg ggtctggctg
     1681 ccgtcacggc gctcgccggc gcggcccgcc accaactcgc ggtcgccaac tggctgcggg
     1741 cggcgttccg gtccgtgcaa ctcatcggct ggcacagcgc cgacacgggc gaggcgctgc
     1801 ctcgtgacac ccccaccggc gacgtggtcg ccacagtcgc ctcggactcg atgcggctgg
     1861 gtggggccta cgacaccacc gcgcggttcg ccggagcgat cgtgtcctac atcgtcgtcg
     1921 ccgtcatcct ccttcgcgcc tcgatgccgc tcggcctgct tgtcctcatc ggcgtgccgg
     1981 tcctcgtcgg tttgctgtcc ttcatcgtcc ggccgctgca gcgccgccag gcccagcagc
     2041 gcgaggagtc cggtcggctg atcggcctcg gggccgacac cgtggctggg ttgcgggtgc
     2101 tgcgcggcat cgggggcgag acgacgttcc ttgcccggta tgccgcccag agccagcagg
     2161 tgcgcgaggt cggctatcgg gtggccggcg tccaggctgc cctcgatgcg gcccaggtgc
     2221 tcctgccggg catctttgtt gtcctcgtca cgtggcttgg ggctcggttc gcgatcgacg
     2281 gccggatcac gcccggtcag ttggttgcgt tctacggcta ctcggccttc ctcgtcaccc
     2341 cgctgcgcac cgcgaccgag ttcgtcgacc ggttgacccg cgcccacatc ggcgcccgca
     2401 agatcatccg ggtgctcaag gtcgagcctg atcacgtcga tcacgccgat gtcgtgactc
     2461 ctggtgccac cgagcctccc gccgactccg ccctgcgcga cgaacgcagc ggcgtcgtcg
     2521 tcgagcccgg acagttcctc gcggtggtca gcggacgccc cgaggagtcg gccgcgctcg
     2581 ccgaccggtt ggggcgcttc ggaaccgacg tcgagggggt gacgctgggc ggcatacccc
     2641 tcactcactt gccgctcgcg acggtgcggc gccggatcct cgtgagcgag accgacccgc
     2701 gcctgttcac cggggtgctg cgcgacgagc tcgacccgtg gggcgcccac tccgacgagg
     2761 agatcctcgc ggccctgacg atcgccagcg gcgaggacgt cctggaggcg ctcgctgacg
     2821 gcctggacag cgaggtcgaa gagcgcggcc ggtcgttctc cggcggccaa cgccaacgcc
     2881 tggcgctgac ccgggcgcta ctggccaacg cgccgatcct cgtgctcgtc gagccgacca
     2941 gtgccgtcga cgcccacacc gaggcccgca tcgccgaccg gctggcgacc ttccgggccg
     3001 ggcggacgac ggtcgtcatg accgccagcc cgctcgtcct cgatcgggcc gaccgcgttc
     3061 tgctgctgca ggacgacgcc atcctcgctg aggggaccca ccacgagctc atgcaccgac
     3121 gcgacggtgc gagcgcggca taccgtgcgg tggtcgtgcg cggcgccgac gaggaggaga
     3181 cccgctgatg tccgtctccg ccggagtccc gagcgcccga cgcactctgc ccgtggccac
     3241 gctggcctcg gtgcgtgccc agacggcggc gctgttgcgc gagcaccgcg gcgcgctcat
     3301 cgaggtcgtc gggctgcatg ccatcgccgc ggtcgcaggg ctggcggcgc cgttcgtcgt
     3361 ggggcggctc gtcgacgagg tcactgcggg cacggcatac gagagcgtcg accggcaggc
     3421 cctgtggctg gccggtgcga tcctcgccca gacagtgctt gtgtgggctg cgcggcggtc
     3481 gtcgttccgg ctcggcgagc gggtcttcgc ccagttgcgt gagcagttca tgacgcgggt
     3541 gatggcgctg ccgctctcga ccgtcgagcg ggcgggcacc ggtgacctcg tatcgcggac
     3601 gaccaacgac gttgaggcgc tcagccacgt cgtccggttc ggcatcccgt cgatcttcgt
     3661 cgcggtggtc acgctggcga tcactgtcgc ggccgcggtg gccaccgggt ggctcgtcgc
     3721 gctgcccgtc gtcgtcggcg tgccgatcat ctggctctcg acccggcgct atctgcgctt
     3781 tgcccccgac gg
//
LOCUS       DAOMZY010000335         3785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_23088_length_3785_cov_4.257641, whole genome shotgun sequence.
ACCESSION   DAOMZY010000335 DAOMZY010000000
VERSION     DAOMZY010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23088_length_3785_cov_4.257641"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PLK64_14805"
     CDS             <1..559
                     /locus_tag="PLK64_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amine oxidase"
                     /protein_id="HPV81062.1"
                     /translation="VRVVQRHADAVSVIDASGGRTEVERVVIATHPDQALTLLADPVP
                     LEASLLGSFRYATNEAVLHRDPSLLPTASGAKASWNYLVQGDGDGPPLVTYWMNRLQG
                     LPSTDQLFVTLNGSDRIHPESVIATMSYAHPLYDLAAVRAQRRLPEISSGRTAFAGAY
                     HGWGFHEDGARSGVAAAAALGVTWG"
     gene            564..2558
                     /locus_tag="PLK64_14810"
     CDS             564..2558
                     /locus_tag="PLK64_14810"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 family protein"
                     /protein_id="HPV81063.1"
                     /translation="MTEPLLPVPALVVGTVRHTRFRPLRASFAHRHYQWLVDLDHPPR
                     LPARLRRLSELRAEDHLDGRASTLAELKALVLQEIRAGGVELPDSTRVLLLAHARVFG
                     HVFDPLSVFWCLAPDGRVVAAILEVRNTYAGRHTYVLTPDPQGKAEVDKDFLVSPFND
                     TTGRYAVRLHLTRDRVSVAIRLERNGAQLLSATVTGRPVPLTGASLTRVLVRHPLMPQ
                     RVSALIRVHGVALWLRRLPVLREKPANGADAQAAPGVDPRIAPAGRHTRVTRGVVARN
                     LVRLLVRSLPIQLRLPGGGTWGAGGANAPVLQVNRPAALFRRLETHPKIGLGEAFMAG
                     DWEAGEGTDLADLLRPFAARVDRLLPRPVLALRGIVERAIPHHHRNTPDGARANIAAH
                     YDLSNDLFAGFLDETMTYSSALFEGSRPWETQDLATAQRRKIDAALDAANVRAGTRML
                     EIGTGWGELALRAAARGASVTTITLSREQQALARQRVLEAGLADRVDVQLRDYREVTG
                     QFDAVVSIEMIEAVGEEFWPAYVETIDRVLAPGGSAVVQSILMDHDRFLATRNSFGWI
                     QKHIFPGGIIPSLEALEEVATRHTSLRVKGIRTFGAHYAHTLRRWRRTFTDNWPQIAT
                     HGFDDVFRREWEFYLAYCEAGFATGYLDVAQITFGRSGPS"
     gene            2555..3541
                     /locus_tag="PLK64_14815"
     CDS             2555..3541
                     /locus_tag="PLK64_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPV81064.1"
                     /translation="MTHPTWTDRLARLVDTALDRSVVLGYPATGHAVRRRLPTWPKDP
                     PRNALSGKHVLVTGASSGLGVRTAVDLAMLGAHVHLVVRDPMKAAPVREQLDEVAAGR
                     HTTWRCDLSDQASVQAFAAAYLATGLPVDGLVHNAGIMPPTRQEDAQGRELTMSVHVL
                     GPVALTEALLPALAAARDGARVVFVTSGGMYSQALDLDDLDFRPEPYRGAVAYARSKR
                     AQVELLPILARRWGRHGISVYAAHPGWAASPGVTTSLPAFARLTAPVLRRGAAGVDTT
                     TWLIAATPAPPSGGLWHDRSERPTSYLSRTRASDAERQELWQWVARHADIAD"
BASE COUNT          531 a         1333 c         1299 g          622 t
ORIGIN      
        1 ggtccgggtc gtgcagcggc atgctgacgc agtctcggtg atcgatgcgt cgggaggccg
       61 caccgaggtc gaacgggtcg tcatcgccac ccaccccgat caggcgctga cgttgctggc
      121 tgacccggtg ccgttggagg cctcgttgct tggctcgttc cggtacgcca cgaacgaggc
      181 tgtcctgcat cgcgatccgt ccctgctgcc gaccgcgtcc ggggcgaaag cgtcgtggaa
      241 ctacctcgtc cagggcgacg gcgatgggcc gccattggtc acctactgga tgaaccgtct
      301 gcagggactg ccgagcaccg accagttgtt cgtcaccctc aacggttccg accggatcca
      361 ccccgagagt gtcattgcga cgatgtccta cgcccaccct ctctatgacc tagcggcggt
      421 tcgcgcacag cgccggctgc ctgagatcag cagcggcaga acggcattcg ccggggccta
      481 tcacgggtgg ggattccacg aggacggcgc ccgctccggc gtggccgccg cggcggctct
      541 cggggtgacg tgggggtgac ctggtgactg aacccctcct gccggtgccg gcgctcgttg
      601 tcggcaccgt gcggcatacc cgcttccgcc ccttgcgggc ttcgttcgcc caccggcact
      661 accaatggct cgtcgacctc gaccacccac cgcgcctgcc ggcccggctg cgtcgcctgt
      721 ccgaactgcg cgccgaggac catctcgatg gtcgagcgtc gaccctggcg gagctgaagg
      781 cccttgtcct gcaggagatt cgcgctggtg gggtcgagct gcccgactcg acaagggtgt
      841 tgctccttgc tcatgcccga gtgttcggac acgtctttga cccgttgtcg gtcttctggt
      901 gtctggcgcc ggacgggcgg gtcgtcgcgg ccattctgga ggtccgcaac acctacgccg
      961 ggcgccacac ctacgtcctg acccccgacc cgcagggcaa ggccgaggtc gacaaggact
     1021 tcctcgtctc gccgttcaac gacaccaccg gcaggtatgc cgtgcgcctg cacctcacgc
     1081 gcgatcgggt gtcggtggcg atccgcttgg agcgcaacgg tgctcaactc ctcagtgcca
     1141 ccgtcaccgg tcgcccggtc ccgctgaccg gggcctcctt gacccgggtc cttgtccggc
     1201 acccgttgat gccgcagcgg gtgagcgccc tgatccgggt ccacggtgtt gccctctggt
     1261 tgcgccgact gccggtcctg cgtgagaagc cggcgaacgg tgcggacgct caggctgccc
     1321 ctggggtcga ccctcgaatc gcgccggcag ggcggcatac ccgggtcacg cgtggtgttg
     1381 tggcccgcaa cctggtccgc ctgctggtgc gcagcctccc tatccagctg cgtcttccgg
     1441 gcggtggcac gtggggcgcg ggcggtgcga atgctcctgt gctgcaagtc aaccggccgg
     1501 ccgcgctgtt ccggcgactc gagacccacc cgaagatcgg cctcggcgag gccttcatgg
     1561 cgggtgactg ggaggcgggc gaaggaaccg atctggccga cctgctcagg ccgttcgccg
     1621 cgcgggtgga ccggctcttg ccgcgaccgg tcctggcgct gcgcggcatt gttgagcggg
     1681 cgatcccgca ccaccaccgc aacaccccgg acggggcgcg cgccaacatc gccgctcact
     1741 acgacctgag caacgacctg ttcgcgggct tcctcgacga gacgatgacc tacagcagtg
     1801 cgctgttcga gggctccagg ccgtgggaaa cgcaggatct cgcgactgcc caacgccgca
     1861 agatcgacgc agccctcgat gccgccaacg tccgggcggg cacgcggatg ctcgaaatcg
     1921 gcacggggtg gggcgaattg gcgttgcggg ctgcggcccg aggcgcgtcg gtcaccacga
     1981 tcaccctctc tcgggagcag caggcgctag ctcggcagcg ggtgctggag gccgggctag
     2041 ccgaccgggt ggacgtgcag ttgcgcgact accgcgaggt gaccgggcag ttcgacgcgg
     2101 tcgtcagcat cgagatgatc gaggcggtcg gcgaggagtt ttggcccgcc tacgttgaaa
     2161 cgatcgaccg agtgctggcg cctgggggat cggcagtcgt ccagtcgatc ctcatggatc
     2221 acgaccgctt tctcgcgacc cgcaactcgt tcggctggat ccagaagcac atcttcccgg
     2281 gcgggatcat cccgtcgctg gaggcgctcg aggaggtggc tacgcggcat acctcgttgc
     2341 gggtcaaggg gattcgcacg ttcggtgcgc actatgccca caccctgcgg cgctggcggc
     2401 ggaccttcac cgacaactgg ccgcagatcg ccacccacgg cttcgacgac gtcttccgcc
     2461 gtgagtggga gttctatctc gcctactgcg aggcggggtt cgccaccggc tacctcgacg
     2521 tcgcccagat cactttcggc aggagcggac cgtcatgacc cacccgacgt ggaccgaccg
     2581 gctggcccgg ctcgttgaca cggcactgga tcggagcgtc gtgctcggct acccggcgac
     2641 cggccacgcc gtgcgccggc gactgccgac ctggccgaaa gacccgcccc gcaacgctct
     2701 gagcggcaag cacgtcctgg tcactggggc gtcgtcgggc ctgggggtcc gaacggccgt
     2761 ggatctcgca atgctcggtg cccacgtgca ccttgtcgtg cgcgacccga tgaaggccgc
     2821 cccggtccgc gagcaactcg acgaggtggc ggcgggacgg cataccacct ggcggtgtga
     2881 cctctccgac caggcgagcg tgcaggcctt cgcggcggcc tatctcgcga cggggctgcc
     2941 ggtcgacgga ctggtccaca acgccgggat catgccgccg acccgccagg aggatgccca
     3001 gggtcgggag ctaacgatgt cggtgcacgt cctcggcccg gtcgcgttga ccgaggcgct
     3061 gctgcccgcc ctggccgccg ctcgtgacgg ggcgcgggtc gtgttcgtca cctccggcgg
     3121 gatgtactcg caggcgctgg acctggacga cctggacttc cgccccgagc cctatcgcgg
     3181 cgcggtcgct tacgcgcgca gcaagcgggc ccaggtggag ttgctcccga tcctcgcccg
     3241 ccgctggggt cgccacggca tcagcgtgta tgccgcccac cccggttggg cggccagccc
     3301 gggcgtcacc acctcgctgc cggccttcgc acggctgacc gctccggtgc tgcgccgcgg
     3361 cgcggctggc gtcgacacga cgacctggct catcgcggcc acccccgcgc cgccgtcagg
     3421 ggggttgtgg cacgaccggt ctgagcgtcc gacgagctat ctgtcccgca cccgggccag
     3481 cgatgccgag cgccaggagt tgtggcagtg ggtcgcccgg cacgcagaca tcgccgactg
     3541 aatcgcccgg ccgccaaaac gtggacttcg gccatgtcgt acgacaggct gtgcccagca
     3601 agccgggatc gccggcagtc gggaggcact cgtggcaatc gtgcgctaca tcgtggacga
     3661 cgtggacacg tccctgactt ttttggaaac cctcggcttc accctcgtgg accgcatggg
     3721 acctcccttc gccatcgtgg agcgggacgg ccttgagctg tgggtcagcg gccccggaac
     3781 atcgg
//
LOCUS       DAOMZY010000336         3784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_23097_length_3784_cov_4.496380, whole genome shotgun sequence.
ACCESSION   DAOMZY010000336 DAOMZY010000000
VERSION     DAOMZY010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23097_length_3784_cov_4.496380"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /gene="arsB"
                     /locus_tag="PLK64_14820"
     CDS             <1..895
                     /gene="arsB"
                     /locus_tag="PLK64_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HPV81065.1"
                     /translation="GLLLMMYPVLAKVRYDEIGLVTADRRMIAASLLLNWVVGPLVMF
                     ALAWWLLGDHPAYRTGVILVGLARCIAMVLLWNDLACGDREMAAVLVALNAVFQVLMY
                     AVLGYFYLQVLPGWLGWEQAALDVSMWGIASIVLVFLGIPLVAGWLTRWWGERARGRA
                     WYEGVFLPRIGPIALYGLLFTVVVLFALQGHTITSEPLAVVRIALPLLVYFAVMWVLG
                     YAVGRLLGLTYARTTTMAFTAASNDFELAIAVAIGVFGVASGQALAGVVGPLIEVPVL
                     VGLVYVALWLRGRVRWPESSG"
     gene            1011..1688
                     /locus_tag="PLK64_14825"
     CDS             1011..1688
                     /locus_tag="PLK64_14825"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPV81066.1"
                     /translation="MTARPLAHLVHPSWGDALGPLEPTVTALGAFLREEVAAGRGYLP
                     AGEHVLRAFTQPLDAVRVLVVGQDPYPTPGHPVGLSFSVAPEVRPLPPSLVNIFAELQ
                     TDLGVPRPSTGDLTPWADRGVLLLNRVLTVGPGKPGSHRGRGWEDVTAAAIAALVARG
                     GPLVAILWGRDARSLAPALGDVARIESAHPSPLSARSGFFGSRPFSRANDLLVGQGAE
                     PIDWSLP"
     gene            1734..2921
                     /locus_tag="PLK64_14830"
     CDS             1734..2921
                     /locus_tag="PLK64_14830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017197353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPV81067.1"
                     /translation="MSEVFLYDAVRTPFGRYAGALAGIRPDDLGALVIRETVRRAPSL
                     EPERIDEIFFGCANGAGEDNRNVGRMSGLLAGVPTSVPATTLNRLCGSSLDAAMVASR
                     QIALGEADVVVVGGVESMSRAPWVLPKTERPYPAGSMELASTTLGWRLVNPAMPKEWT
                     VSLGEATEQLREKYAVTREAMDEFAARSHFLADAAWSSGFFADQVVAVPGVGLDRDES
                     IRCETTVERLAGLKPSFRSAETGTVTAGNASPLNDGASAALLGSAKADALTGLSPVAR
                     IAGRGAAANDPQFFGYAPVEAANQALARAGITWDDVDAVELNEAFAAQALACVAAWGV
                     DPEIVNQHGGAIAIGHPLGASGTRILGTLARSLQVSGGKWGVAAICIGVGQGLAVVIE
                     NVS"
     gene            3242..>3784
                     /locus_tag="PLK64_14835"
     CDS             3242..>3784
                     /locus_tag="PLK64_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPV81068.1"
                     /translation="MAATRLMPSDESRALIEMTREVCRKELAPRVDAFERDGGFPEQA
                     FRTLGGLGLLSLPYPEEFGGGGQPYEVYLQVVEEIATVWMSVAVGVSVHALTCYPIAE
                     YGTEEQRHEFLPDMLSGDWLGGYCLSEPDSGSDVGSMTTQAERTDDSPSGTGGGTGTS
                     TGGGWRIDGTKAWISHAGHAN"
BASE COUNT          496 a         1300 c         1352 g          636 t
ORIGIN      
        1 cgggctgttg ctgatgatgt accccgtcct ggccaaggtc cgctacgacg agatcggcct
       61 ggtcaccgcc gaccgccgga tgatcgcggc atcgctgctg ctcaactggg tagtcggtcc
      121 cctggtgatg ttcgccctgg cctggtggct gctcggtgac cacccggcat accggaccgg
      181 ggtgatcctc gtcgggctgg cgcggtgcat cgccatggtg ctcttgtgga acgacctcgc
      241 gtgcggcgac cgcgagatgg cggccgtcct cgtggccctc aacgcggtgt tccaggtgct
      301 catgtatgcc gtgctcggct acttctatct gcaggtcctg ccggggtggc tggggtggga
      361 gcaggcggcg ctcgacgtgt cgatgtgggg catcgcctcg atcgtcctcg tcttcctcgg
      421 catcccgctc gtcgccgggt ggctgacccg gtggtggggc gagcgagccc gcgggcgggc
      481 ctggtacgag ggcgtgttcc tgccccggat cggcccgatc gcgctctacg ggctgctgtt
      541 caccgtcgtc gtgctgttcg cgttgcaggg tcacacgatc acctccgagc cgctcgcggt
      601 ggtccgaatc gccctgccgc tgctcgtcta cttcgcggtc atgtgggtgc tcgggtatgc
      661 cgtggggcgg cttctgggac tgacctacgc ccgcacgacg acgatggctt tcaccgccgc
      721 cagcaacgac ttcgagttgg cgatcgccgt cgccatcggg gtgttcggcg ttgcgtcggg
      781 ccaggccctg gctggggtcg tcggcccgct catcgaagtg cctgtcctcg tcggcctggt
      841 ctatgtcgcg ctctggttgc ggggtcgggt gcgctggccc gaaagctccg gctgagcccg
      901 acgggccggg acggtcgacc ggacctgcgg gcgaggtcga ccgaccggcg accggcgggt
      961 gcgtcacacc ggcgggcaca cggcataccg gggcgggtaa gttctgcgac gtgactgcac
     1021 ggccgctggc gcacctcgtc cacccctcgt ggggtgacgc gctcgggccg ttggagccga
     1081 ccgtgaccgc gctgggtgcc ttcttgcgcg aggaggtggc ggccgggcgc ggctacctgc
     1141 cggccgggga acacgtcctg cgggccttca cccaaccgct tgacgcggtg cgcgtgctcg
     1201 tcgtcgggca ggacccctac ccgacgcccg ggcacccggt ggggctgagt ttctcggtgg
     1261 cgcctgaggt gcggccgttg ccaccgagcc tggtgaatat cttcgccgag ttgcagaccg
     1321 acctcggcgt gccacggccg agcaccggcg acctgacgcc ctgggccgat cgcggagtcc
     1381 tgctgctcaa ccgggtcctc accgtcggcc cgggcaagcc gggcagccac cgcgggcggg
     1441 gctgggaaga cgtcaccgcc gccgccattg ccgcgctggt cgcccggggt ggcccgctgg
     1501 tcgcgatcct ctggggtcgc gatgcgcgct cgctcgcccc ggcgctcggg gacgtcgccc
     1561 gcatcgagag cgcccatccc tcgcccctgt cggcccgctc cggcttcttc ggatcgcggc
     1621 cgttcagtcg cgccaacgac ctgctcgtgg gtcaaggcgc cgagcccatt gactggagcc
     1681 tgccgtgagc gggctggagt cacgccgtcc cacccagcat ggaggttgca gtcatgagtg
     1741 aggtcttcct ctacgacgcg gtccgcacgc ccttcggccg gtatgccggt gcgctggccg
     1801 gcatccgacc cgacgacctc ggggcgctcg tcatccgcga gacggtccgc cgggctccct
     1861 cgctcgaacc ggagcggatc gacgagatct tcttcggctg cgctaacggt gctggcgagg
     1921 acaaccgcaa cgtcggccgg atgtccggac tgctcgctgg ggtgccgaca tcggtgcccg
     1981 ccacgacgct caaccgcctg tgcgggtcga gcctggacgc tgccatggtc gcctcccggc
     2041 agatcgccct cggcgaggcc gacgtcgtcg tcgtcggcgg ggtcgagtcg atgagccgcg
     2101 ccccgtgggt gctgcccaag accgagcggc cctatcccgc cggctcgatg gagttggcct
     2161 cgaccacgct cggctggcgg ttggtcaacc cggcgatgcc gaaggagtgg accgtctcgc
     2221 tcggcgaggc caccgagcaa ctgcgcgaga agtatgccgt gacccgcgag gcgatggacg
     2281 agttcgccgc ccgctcacac ttcctcgccg atgccgcctg gtcgagcggc ttcttcgccg
     2341 atcaggtcgt cgctgtgccc ggggtcgggc tggatcgcga cgagtcgatc cggtgcgaga
     2401 cgacggttga gcggttggcc gggcttaagc cgtcgttccg gtccgccgag acggggaccg
     2461 tgacggccgg caacgcctcg ccgctcaacg acggtgcgtc cgcagcgctg ttggggtcgg
     2521 ccaaggccga tgcgctcacg ggtctgagcc cggtggcgcg gatcgcaggt cgtggggccg
     2581 ccgcaaacga cccgcagttc ttcggctacg ccccggtcga ggcggccaac caggctctgg
     2641 ctcgggccgg catcacgtgg gacgacgtcg acgccgtcga actcaacgag gcgttcgccg
     2701 ctcaggcctt ggcctgtgtc gcggcgtggg gcgtggaccc cgagatcgtc aaccagcacg
     2761 gcggggcgat cgccatcggg cacccgttgg gcgcatccgg cacccggatc cttggcaccc
     2821 tcgcccgatc gctgcaggtc agcggcggca aatggggagt cgccgcgatc tgcatcggag
     2881 tcggccaagg cctcgccgtc gtcatcgaga acgtctcctg acccacgcga gctgagcgca
     2941 cggcatacct cccctggctc agccacggag atagcgctcg catggtcatc tttccggggt
     3001 ttttggcctc aacccgacgg agaagtgacc gtgcgagcgc tatctccgga gtggggtccg
     3061 aaacaagggg tgtccacaga gatagcggtt gcctggtcat ctttgggggc atctcggccc
     3121 gaatctgacg gaaaagtgac cgtgcgagcg ctatctccgg gtttgaggcc ggggtgggag
     3181 ccaggcgggt gtgacatgtg caccaccaca aatgcttgga cgtcctagta ttctcggcat
     3241 catggcagca acgaggctga tgccgtcgga cgagtcccga gcgctcatcg agatgacccg
     3301 ggaggtatgc cgcaaagagc tcgctccccg ggtcgacgcc ttcgagcgcg acggtggctt
     3361 ccccgagcag gcgttccgaa ccctgggcgg cctggggctg ctctcgctgc cctaccccga
     3421 ggagttcggc ggcggcgggc agccctacga ggtctacctg caggtcgtcg aggagatcgc
     3481 caccgtctgg atgtcggttg cggtgggcgt cagcgttcac gcgctcacgt gctaccccat
     3541 cgccgaatac ggcaccgagg agcagcgcca cgagttcctg cccgacatgc tcagcggcga
     3601 ctggctcggc ggctactgcc tctcggagcc cgactcgggg tcggatgtcg gctcgatgac
     3661 cacccaggcc gagcgcaccg acgacagccc gagcggcacc ggcggcggca ccggcaccag
     3721 caccggcggc ggctggcgaa tcgacggcac caaggcgtgg atctcccatg cggggcacgc
     3781 caac
//
LOCUS       DAOMZY010000337         3751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_23417_length_3751_cov_5.524621, whole genome shotgun sequence.
ACCESSION   DAOMZY010000337 DAOMZY010000000
VERSION     DAOMZY010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23417_length_3751_cov_5.524621"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="PLK64_14840"
     CDS             <1..788
                     /locus_tag="PLK64_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017200425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HPV81069.1"
                     /translation="AAAARATVADPEVERALEAPLPDLAALAAKMNDARGQRTAAARE
                     HALAEQAARDIDVLSRQVVANADAAAAATRVAASTAALADVVSGLGSDNTLRMKLSAF
                     VLAGRLERVVELANERLQRMGDGRFHLAHSDRLASGGRRSGLGLVVQDEWTGQVRDTA
                     TLSGGESFMASLALALGLADAVREESGGIDLHTLFIDEGFGSLDGESLEQVMEVLDQL
                     QDGGRAVGVVSHVPALRDRIPAQVQVDKTRRGSTVRRVTGPAA"
     gene            complement(789..2879)
                     /locus_tag="PLK64_14845"
     CDS             complement(789..2879)
                     /locus_tag="PLK64_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HPV81070.1"
                     /translation="MGKKHRREQKLAKQLAKELAKDEQRVGSPIPESLRPRPALSYEL
                     DDAKESVALSALRRVTPGLTYLLTQYPRGMIRRDAVAGVAVAAYLIPQVMAYSAIVNV
                     PPVASLWTALAAIIAYAVLGSSRILSSGPESTIALMTGVAIAPLAASNPDRAVALSAA
                     LALCVAGWCLIARVLRAGIIAELLSTPLLVGYLAGGAVLMIVGQLGKVTGTSVDGESI
                     VEQLQSFFSVVGRTQMPTLIVGVATLALILVIITFRPKWPAPLIAVVLATVASVLLHL
                     EAQGVKVVGTVPSGLPMPHLPMVTGEEFKAMIWAGLGIAIVGYSDIMLISRGFPLTPG
                     EGETKADVRADPQQELVAMAGVHAFVGFFSGYPVSASGSRTALAVAGGARSQAYSLVA
                     GLCVVAVLFFAGPLMAGLPSAALGAVVFYAAGKLVSIPDFKRLWAFRRREFFLAIIAM
                     LGTVVIGILQGVLFAIALSLLEMLHRLARPHEGVLGRVPGIPGMHDVADYPDARTIPG
                     CIFYRYDAPLFFANVGDLRERVDRVISVENQAYPQTPVRWFILNVEANVEVDITAADA
                     LRELAQELADRGIELGLARVKFDLYEPLDRAGVIDVIGKDMLFATLPVAEEAYLKWAV
                     AQANSGSVEPDLEVVQAAVDSVPAHSLLPWDRPDDEEDDDGPRPNLGPAEARVAVLDP
                     ETARPDQPADGAAR"
     gene            complement(2980..>3751)
                     /locus_tag="PLK64_14850"
     CDS             complement(2980..>3751)
                     /locus_tag="PLK64_14850"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155859.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HPV81071.1"
                     /translation="NLHKTFCIPHGGGGPGVGPVAVRSHLAPYLPNHPLAPECGPSTG
                     VGPVSAAPYGSASVLPISWAYIRLMGGAGLTRATQVAILNANYIAARLREHFPVLYAG
                     ASGLVAHECILDLRGITAETGVTVDDVAKRLVDYGFHAPTMSFPVAGTLMVEPTESED
                     LGELDRFIAAMISIRDEINAVRDGVITAEASPLRSAPHTAYALTGEWDRAYDRTTAVF
                     PPGVDPTDKYWPPVARIDGAYGDRHLVCSCPAPEAFED"
BASE COUNT          628 a         1263 c         1331 g          529 t
ORIGIN      
        1 ccgcggccgc agcacgggcc accgtcgccg atcccgaggt cgagcgcgcg ctggaagccc
       61 cattgcccga cctcgcggcc ttggcagcca agatgaacga cgcccggggc cagcgcaccg
      121 ccgcggcacg cgagcacgcc ctcgccgagc aggccgcgcg cgacatcgac gtgctttcgc
      181 ggcaggtggt tgccaacgcc gatgccgcgg cagccgcaac gcgggtggcg gcctcgaccg
      241 ccgcgctggc cgatgtcgtg tcggggctcg gctcggacaa cacgctgcgg atgaagctct
      301 cggccttcgt gctggccggg cggttggagc gggtcgtcga gttggccaac gagcgactcc
      361 agcggatggg cgacgggcgc ttccacctgg cccacagcga ccggctcgcg tccgggggac
      421 ggcgcagtgg cctgggcctg gtcgttcagg acgagtggac cggacaggtc cgcgacaccg
      481 ccaccttgtc cggcggcgag tcgttcatgg cctccctggc tctggccctc gggttggccg
      541 acgcggtccg cgaggagagc ggcggcatcg acctgcacac gctgttcatt gacgaggggt
      601 tcggcagcct cgacggcgag agcctcgaac aggtgatgga ggtgcttgac caactgcagg
      661 acggtgggcg cgcggtcggg gtcgtcagcc acgttccggc cttgcgtgac cggatcccgg
      721 ctcaggtgca ggtcgacaag accaggcggg gctcgacggt ccggcgagtc accggcccgg
      781 ccgcctgatc acctggccgc accgtcggcg ggttggtccg ggcgggcggt ttcgggatcg
      841 agcacggcca ctctcgcttc ggcgggcccg aggttcggcc gcgggccgtc gtcatcctcc
      901 tcatcatcgg ggcgatccca cggcagcagg ctgtgcgccg gcacggagtc gaccgcggcc
      961 tgcaccactt cgagatcggg ctcgaccgat ccggagttcg cttgggccac agcccacttg
     1021 agataggcct cttcggccac gggcagggtg gcgaagagca tgtccttccc gatgacatcg
     1081 atgacgccgg cccggtcaag cggctcgtag aggtcgaact tcacccgagc caggccaagc
     1141 tcgatgcctc ggtcggccaa ctcctgggcc aactcgcgca gggcgtccgc ggcggtgatg
     1201 tcgacctcga cattggcctc cacgttgagg atgaaccagc gcaccggagt ctgcgggtag
     1261 gcctggttct ccacggaaat gacccggtcg acccgttcgc gcaggtcccc cacattggcg
     1321 aagaacagcg gcgcgtcata gcgatagaag atgcatccag gaatcgtgcg ggcatcggga
     1381 tagtcggcga catcgtgcat ccccgggatc ccaggaaccc gcccgaggac gccctcgtgg
     1441 ggcctggcga ggcggtggag catctccagc aggctcaggg cgatcgcgaa gagcacgccc
     1501 tgcaggatgc cgatgacgac ggtgcccagc atggcgatga tggcgaggaa gaactcgcgg
     1561 cgccggaagg cccacagccg tttgaagtcg gggatcgaca cgagtttccc ggcggcatag
     1621 aacaccaccg cgcccagcgc ggccgatggc agacctgcca taagtgggcc ggcgaagaac
     1681 aacaccgcaa caacgcacag cccggccacg agcgaatacg cctggctgcg tgcgcctcct
     1741 gccacggcca gtgcggtgcg cgagcccgac gcagacaccg ggtaaccgct aaagaagccg
     1801 acgaaggcat gcacccccgc catcgcgacc aactcctgtt gggggtcggc gcgcacatcg
     1861 gccttggtct ccccctcgcc cggggtcagc gggaagccgc gcgagatgag catgatgtcg
     1921 ctgtagccga cgatggcgat gccgagcccg gcccagatca tcgccttgaa ttcctcgccc
     1981 gtgaccatcg gcaggtgcgg catcggtagc cccgacggca cggtcccgac gaccttcacc
     2041 ccctgggctt ccaggtgcag caggaccgac gcgactgtgg cgagtacgac cgcgatgagc
     2101 ggggcaggcc acttgggccg gaacgtgatg atgaccagga tgagcgccag ggtggcgacg
     2161 ccgacgatga gcgtgggcat ctgcgtccgc ccgacgacgg agaagaacga ctgcagttgc
     2221 tcgacgatgc tctcgccgtc gacggaggta cccgtcacct tgcccagttg gccgacgatc
     2281 atcagcaccg caccaccggc gaggtagccg acgagcagcg gggtcgacaa caactcggcg
     2341 atgatccccg ctcggagcac ccgggcgatg agacaccacc cggcgacgca cagggcgagc
     2401 gccgccgaca acgcgacagc ccggtcaggg ttgctcgctg ccagcggggc gatggccacg
     2461 ccggtcatca gcgcgatcgt cgattcaggt cctgacgaga ggatccgcga gctgccgagc
     2521 acggcatacg cgatgatcgc ggcgagcgcg gtccacaggc tcgcgacggg cgggacgttg
     2581 acgatggctg agtaggccat cacctgcggg atgaggtatg ccgcgaccgc tacgcccgcg
     2641 acagcgtccc ggcggatcat cccgcgggga tactgcgtca gcaggtaggt gaggccaggg
     2701 gtgacccgcc gcaacgcgga cagggcaacg ctctccttgg cgtcgtccaa ctcgtaggac
     2761 agcgccggac gcggacgcag gctttcgggg atcggtgacc cgacgcgctg ctcgtccttg
     2821 gccaactcct tggccaactg cttggcaagc ttctgctcgc ggcggtgctt cttgcccacc
     2881 cgggaactcc tcaagtcgtg ccgcagcgtt gtgtggcgtt caccttgtgt ttgcccacag
     2941 gctatcggca ggaccctatc aagacagaaa cgcgcccagt cagtcctcga aagcctccgg
     3001 cgccgggcac gagcagacca ggtgccggtc accgtaggca ccgtcgatgc gcgccaccgg
     3061 cggccagtac ttgtcggtcg ggtccacccc gggcgggaag accgcggtcg ttcggtcgta
     3121 ggcgcggtcc cactcgcccg tcagcgcgta tgccgtgtgc ggagccgagc gcaacgggct
     3181 ggcttcggcc gtgatgaccc cgtcacgcac ggcgttgatc tcgtcgcgga tcgagatcat
     3241 cgcggcgatg aaccgatcca actcgcccag gtcctcactc tcggtcggtt cgaccatgag
     3301 cgtcccggcc acggggaaag acatcgtggg ggcgtggaag ccgtagtcga cgaggcgctt
     3361 ggcgacgtcg tcgacagtga cgcccgtctc cgcggtgatg ccccgaaggt cgaggatgca
     3421 ctcgtgagcc acgagaccgg acgcaccggc atacaacacc gggaagtgct cgcgcaggcg
     3481 ggccgcgatg tagttggcgt tgaggatggc cacctgggtg gcgcgagtca gcccggcgcc
     3541 gcccatgagc cggatgtagg cccacgagat cgggagcacg ctcgccgagc cgtagggggc
     3601 cgccgacacc gggccgacgc cggtcgaggg accgcattcg ggggccagcg ggtggttggg
     3661 gaggtagggc gcgaggtgcg agcgcaccgc aaccgggccg acgcccggcc cgccgccgcc
     3721 atgcgggatg cagaaggtct tgtgcaggtt g
//
LOCUS       DAOMZY010000338         3724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_23642_length_3724_cov_7.418643, whole genome shotgun sequence.
ACCESSION   DAOMZY010000338 DAOMZY010000000
VERSION     DAOMZY010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23642_length_3724_cov_7.418643"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..626
                     /gene="def"
                     /locus_tag="PLK64_14855"
     CDS             18..626
                     /gene="def"
                     /locus_tag="PLK64_14855"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPV81072.1"
                     /translation="MAIRPVVIHGEPVLHRVASRVERFDDELAELVQDMYDTMDAAHG
                     VGLAAPQIGVGLRVFVYDMANQDGVPARGVVVNPILAVSKVPSGRPDPDEESEGCLSV
                     PGEHFPLRRAETAKVNGQDLTGAPFEFTATDWFARCMQHEFDHLNGKLYVDRLDDKQT
                     RKARKAIKNNGWGKPGHSWMPGADPDPFGHGDIDADGDDHDL"
     gene            598..1575
                     /gene="miaA"
                     /locus_tag="PLK64_14860"
     CDS             598..1575
                     /gene="miaA"
                     /locus_tag="PLK64_14860"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPV81073.1"
                     /translation="MPTVTTTTSDVPISPLVVAVVGPTATGKSALAVALALALDGEIV
                     NADASALYRGMDIGTAKPTLAERRGIPHHQLDVLEVTQEASVAAYQQDARADVEAILA
                     RGRLPILAGGSGLYVRAALDHLDIPPTDPAVRARLEAEATADPDGPSHLRSRLAQLDP
                     VAAQRIEPNNVRRIVRALEVIELTGRPFSATMPERRHLLPTVMIGLTLDRASLDARIE
                     ARTRAMWDAGLVAEVEGLLDRGLAQGRTARTALGYAQAIDQLRGALTQTEAIEATAAA
                     TRRFARRQESWFRPDPRIIWLPAEAPDLLGRAQEAVKAASQTSAMPENG"
     gene            1577..2464
                     /gene="dapF"
                     /locus_tag="PLK64_14865"
     CDS             1577..2464
                     /gene="dapF"
                     /locus_tag="PLK64_14865"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPV81074.1"
                     /translation="MSGLAFTKGHGTENDFVLVADPDGRLDLRPEQVRALADRRAGIG
                     ADGVIRVVPTHLADDPDVRAQSGFAPWFMDYRNADGSLSEMCGNGTRVFAAYLVREGL
                     QAPGAFAIATRAGVKAVRTTDTGYAVDLGPARVDESEGAAESGFNAVVHPHGGPELPG
                     LRVDVGNPHTVVVLPPHVALDGLDLTVAPHVAPHPPEGTNVEFVQVVGPGHIAMRVHE
                     RGVGETRSCGTGAVAAATATRWWDGPDDAQHRWTVDVPGGRLTVTFTEAGTAELAGPA
                     VLVADGTWTEGPGSGSVSL"
     gene            complement(2454..3056)
                     /locus_tag="PLK64_14870"
     CDS             complement(2454..3056)
                     /locus_tag="PLK64_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPV81075.1"
                     /translation="MSEHEHYFTATPASPEERRTLTVDLAGRRVTITSAGGIFSPDRV
                     DVGTAVLLAEVPDPPLTGSLLDLGCGWGPVALTLGMLSPAADVYAVDVNERALQLARD
                     NAASLGLNRIQVCRPDEVPADVRFAQIWSNPPIRVGKAVLHDLLRTWLPRLEPGGTAY
                     LVVAKNLGSDSLQRWIATDLGMPCERYVTSRGFRVLTVTG"
     gene            3117..>3724
                     /locus_tag="PLK64_14875"
     CDS             3117..>3724
                     /locus_tag="PLK64_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPV81076.1"
                     /translation="MTRRTDLLAGKATSLADDDLDPHGWGSAAYPGGPGTDFDGDQLD
                     REERAALRRVAGLSTELEDVTEVEYRQLRLERVVLAAVWTEGTVQDAENSIRELAALA
                     ETAGSTVLDGVIQRRQAPDPATFLGKGKAVELRDIVLAEGADTVICDGELAPGQRRAL
                     EDVVKVKVIDRTALILDIFAQHAQSREGKAQVELAQLQYLLPR"
BASE COUNT          582 a         1330 c         1287 g          525 t
ORIGIN      
        1 catgcttgac ttgggccatg gccatccgtc ccgtcgtcat ccacggtgag cccgtcctgc
       61 accgcgtcgc cagccgtgtc gagcgtttcg atgatgagtt ggccgagttg gtccaggaca
      121 tgtacgacac gatggacgcc gcacacggcg tcggcctcgc cgctccgcag atcggcgtgg
      181 gcctgcgggt gttcgtctac gacatggcca accaggacgg cgtccccgcc cgcggagtgg
      241 tcgtcaaccc catcctcgcc gtctccaagg tgcccagcgg ccgtcccgac cccgacgaag
      301 agtccgaggg ctgcctctcc gtccccggcg aacacttccc gttgcgtcgg gccgagacgg
      361 ccaaggtcaa cggtcaggac ctgacggggg cgccgttcga gttcaccgcc accgactggt
      421 tcgcccggtg catgcagcac gagttcgacc atctcaacgg caagctctac gtcgaccggc
      481 tcgatgacaa gcagacgcgc aaggcacgca aggccatcaa gaacaacggg tggggcaagc
      541 cgggacatag ctggatgccg ggcgccgacc ccgacccgtt cgggcacggc gacatcgatg
      601 ccgacggtga cgaccacgac ctctgacgtg ccgatcagcc cgctcgtcgt ggccgtcgtc
      661 ggtccgacgg ccaccggcaa gtcggctttg gcggtcgcat tggccctggc cctcgacggc
      721 gagatcgtca acgccgacgc cagcgcgctc taccgcggga tggacatcgg caccgccaaa
      781 ccgaccctcg ccgaacgtcg cggcatcccg caccaccaac tcgacgtcct ggaggtcacc
      841 caggaggcga gcgtcgcggc ataccagcag gacgcccggg ccgacgtgga ggcgatcctc
      901 gcgcgcggcc ggctcccgat cctcgccggt gggtcggggc tctatgtgcg ggctgcgctc
      961 gaccacctcg acatcccgcc gaccgacccg gccgtccgag cccggctgga ggccgaagcc
     1021 acggccgacc ccgacggacc cagccacctc cgttcccgct tggcgcagct ggatcccgtt
     1081 gcggcgcaac ggatcgagcc caacaacgtc cggcgcatcg tccgggcgtt ggaggtgatc
     1141 gagctgaccg gccgaccctt cagtgcgacg atgcccgagc ggcgccacct gctcccgacc
     1201 gtcatgatcg ggctgacgct cgaccgcgcc agcctcgatg cgaggatcga ggcacgcacc
     1261 cgcgcgatgt gggatgccgg gctggtggcc gaggtggagg gcctgcttga ccgcggcctc
     1321 gcccagggcc gcaccgctcg caccgcactg ggttacgccc aggcgatcga ccaactgcgc
     1381 ggcgcgctca cccagacgga agccattgaa gcgaccgccg ccgccacccg gaggttcgcg
     1441 cggcggcagg agtcctggtt ccgacccgac ccgcgcatca tctggttgcc ggccgaggca
     1501 cccgacctgc tcgggcgcgc ccaggaggcc gtcaaagcgg cgagccagac gtcggcgatg
     1561 cctgagaatg gctgacatga gtgggctggc cttcaccaag ggacacggga cggaaaacga
     1621 cttcgtcctc gtcgccgacc ctgacgggcg cctcgacctg cgcccggagc aggtccgcgc
     1681 cctcgccgac cgccgggccg gcatcggtgc cgacggcgtc atcagggtcg tcccgaccca
     1741 cctcgccgac gaccccgacg tgcgggcgca gtcggggttc gcgccgtggt tcatggacta
     1801 ccgcaacgcc gacggatcgc tgagcgagat gtgcggcaac gggacgcggg tgttcgcggc
     1861 atacctcgtc cgcgagggcc tgcaggcgcc cggcgccttc gcgatcgcta cccgtgcggg
     1921 cgtcaaggca gtccgcacca ccgacactgg gtatgccgtg gacctgggtc ccgcccgggt
     1981 tgacgagtcc gaaggggccg ccgagagcgg gttcaacgcc gtggtccacc cgcacggggg
     2041 tcccgagttg cccgggcttc gtgtcgatgt cggcaacccg cacaccgtcg tcgtcctgcc
     2101 gccgcatgtc gcgttggacg gccttgacct caccgtcgcc ccccacgtgg caccccaccc
     2161 gcccgagggc accaacgtcg agttcgtgca ggtcgtcggc ccaggtcaca tcgccatgcg
     2221 ggtccatgag cggggcgtgg gggagacccg ctcctgcggc acgggtgccg tcgcggcggc
     2281 cacggcgacc cggtggtggg acggtcccga cgatgcccaa caccggtgga ccgtcgacgt
     2341 gcccggtggc cggctcacgg tgaccttcac cgaggccggg accgctgaac ttgccggtcc
     2401 cgcggtgctt gtcgccgacg gcacctggac cgaagggccg gggagcgggt cggtcagcct
     2461 gtgaccgtga gcacccggaa gccgcgcgag gtcacgtagc gctcacacgg cataccgagg
     2521 tcggtggcga tccaccgctg caaggagtca cttccgaggt tcttcgcgac gacgaggtat
     2581 gccgtcccgc cgggttccag ccgcggcagc caggtgcgca gcaggtcgtg caggaccgcc
     2641 ttgcccacgc ggatcggcgg gttggaccag atctgtgcga accggacgtc ggcaggaact
     2701 tcgtcagggc ggcatacctg aatccgattg agccccaacg atgctgcgtt gtcgcgggcg
     2761 agttgcaggg cccgctcgtt gacgtcgacg gcgtagacgt cggccgcggg ggagagcatc
     2821 ccgagcgtga gggcgaccgg tccccacccg cagccgaggt ccaggagcga tcccgtgagg
     2881 ggagggtcag ggacctcggc gagcaggacc gccgtgccca catcgacgcg gtcaggcgaa
     2941 aagatgcctc ccgcgctcgt gatcgtgacg cggcgaccgg ccaggtcaac ggtgagggtg
     3001 cgtcgctcct ctggggaggc cggcgtcgcg gtgaagtagt gttcgtgctc gctcatcgcc
     3061 cctaaaccta aacggttggt cgagtcggcg tttgccgtgc gaggcttaga cccacgatga
     3121 cacgacgaac tgatctgctg gccggcaagg ccacctcgct tgccgatgac gatctcgacc
     3181 cgcacgggtg ggggagcgcg gcctaccccg gcggccctgg caccgacttc gacggtgacc
     3241 aactcgatcg cgaggagcga gccgccctgc ggcgcgttgc ggggctctcg accgagcttg
     3301 aggacgtcac cgaggtcgaa taccgccagt tgcgcctcga acgggtcgtg ctggccgccg
     3361 tctggaccga ggggaccgtc caggacgccg agaactcgat ccgcgagttg gccgcgctcg
     3421 ccgagaccgc cggctcgacg gtgctcgacg gggtgatcca gcgtcgccag gcccccgatc
     3481 cggcgacctt cctcggcaag ggcaaggctg tggagttgcg tgacatcgtc ctggccgaag
     3541 gcgccgacac ggtgatctgc gacggcgaac tagcccccgg ccagcggcgc gcgctggagg
     3601 atgtcgtcaa ggtcaaggtc atcgaccgca ccgcgttgat cctcgacatc ttcgcccagc
     3661 acgcccagag cagggagggc aaggcccagg tcgagctcgc ccaactgcag tacctcctgc
     3721 cccg
//
LOCUS       DAOMZY010000339         3695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_23895_length_3695_cov_6.691758, whole genome shotgun sequence.
ACCESSION   DAOMZY010000339 DAOMZY010000000
VERSION     DAOMZY010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23895_length_3695_cov_6.691758"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="PLK64_14880"
     CDS             <1..584
                     /locus_tag="PLK64_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPV81077.1"
                     /translation="YDISFQHASNPLLRRMRRFGGTDDFLELLARVRAATPHAGVRSN
                     VIVGFPGETEEDLGQLELFLAMGRLDVVGVFGYSDEEGTEGAGLGGKLPADVVAERVA
                     RVTDLVEQLTAQRAEERVGEQVVVLVEEIDEDGIAVGRAEIQGPDVDGTTRLQDGASG
                     MPSGMPLGRVAVGDLVAAVVVGSEGVDLVAEAR"
     gene            629..1255
                     /gene="pgsA"
                     /locus_tag="PLK64_14885"
     CDS             629..1255
                     /gene="pgsA"
                     /locus_tag="PLK64_14885"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HPV81078.1"
                     /translation="MAQPSHGTSPWNLPNALTVLRILLVPVFGSMLLAQNGADAGQRF
                     AALAVFVVAMITDRIDGDIARSRGLVTDVGKILDPIADKALLGMAFVGLSLIGLVPWW
                     VTLLVLAREVGVTVVRFVVIRHGVMPAGRGGKIKTFAQAVALSLLILPAWVLPAPQIW
                     QWTAYSVLAIAVALTVVTGLDYLVQAHRLRRTSPRAMAKRAAREAKKR"
     gene            1252..1731
                     /locus_tag="PLK64_14890"
     CDS             1252..1731
                     /locus_tag="PLK64_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HPV81079.1"
                     /translation="MRATQVLADLAVRGETIACAESLTGGALTARLIDVPGASRVVRG
                     GVVAYAADVKAAVLGVSAARLASHGTVDPQVAAEMALGVCRVLNSDWGVATTGVAGPE
                     PSEGKRVGTAYVAVVRAGGEPVIHELHLAGNRAKIRAAVVDAALEFIDAALAADDAQ"
     gene            1837..2136
                     /locus_tag="PLK64_14895"
     CDS             1837..2136
                     /locus_tag="PLK64_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPV81080.1"
                     /translation="MVLMRRELGDVLREERRAQKRSLRDVSASAAVSLGYLSEIERGE
                     KEASSELLGAICEALGLPLSALLRELSVRVAQAEGFATPVVLPVPSLGDVSASAA"
     gene            2155..3285
                     /locus_tag="PLK64_14900"
     CDS             2155..3285
                     /locus_tag="PLK64_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HPV81081.1"
                     /translation="MARIRAVRSSVLSIPLHTPFVTALRRTSTIDTLIVCLDLADGTT
                     GLGEAPQVWAVTGDSVASATACVEQVLAPVLVGADSDDWDALAAGVARACARNFGAKA
                     AVETALLDAVARAAGMPLAYLFADVAGVPRPVSRGVGLGGGAAGVFEVATDVTISAGS
                     PEALRSAAQTRVAEGFTRLKLKVGTDAATDVARVRAVREAAGPGIGVRLDANQGWSRE
                     DAVAVIRALAEADLGVEIVEQPVAGDDVEGLAWVRERVDLPIMADETVYGATDLERVI
                     RLEAADAVNVKLAKCGGPLTGLRLLARAREAGLGTMVGSMMESHVGVATAASMVAAVG
                     TSAVSDLDAAWWATHSPYAGGPTYGPGVIQLPDALGLGMSLA"
     gene            3503..>3695
                     /locus_tag="PLK64_14905"
     CDS             3503..>3695
                     /locus_tag="PLK64_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81082.1"
                     /translation="MDSSEATRDSASRRAEIRAATRTATELVASLWQVQGAELGSLLG
                     ELDALAAVACGARVAVVGEA"
BASE COUNT          490 a         1188 c         1396 g          621 t
ORIGIN      
        1 actacgacat ctccttccaa cacgcgtcga accccctgct gcgtcggatg cgccggttcg
       61 gcgggaccga cgacttcctt gagttgctgg cgcgggtgcg ggcggcgacc ccgcacgccg
      121 gcgtccgctc caacgtcatc gtcggcttcc ctggcgagac cgaggaggac ctcggccagt
      181 tggagctctt cctcgcgatg gggcggctgg acgtcgtggg agtgttcggc tactccgacg
      241 aggaaggcac cgagggggcg gggctgggcg gcaaactgcc ggccgacgtc gtggccgagc
      301 gcgtcgcgcg ggtcaccgat ctcgtcgagc agctcaccgc acagcgcgcg gaggagcggg
      361 tgggggagca ggtcgtcgtt cttgtcgagg agatcgacga ggacggaatc gcggtggggc
      421 gggcggagat tcaaggcccc gacgtcgacg gcacgacccg actgcaggac ggcgcatcgg
      481 gtatgccgtc gggtatgccg cttggccggg tcgccgtcgg cgacctcgtc gccgcggtcg
      541 tggtcgggtc cgaaggcgtc gacctggtgg cggaggcccg atgagggagc ccgtgccgcc
      601 gcgaccgatg actgggccac cggatccgat ggcccagccc tcccacggca ccagcccctg
      661 gaacctgccc aacgccctca ctgtcctgcg catcctgctc gtccccgtct tcgggtcgat
      721 gttgctcgcg cagaacgggg ccgatgccgg acaacgcttc gcggccctcg cagtcttcgt
      781 cgtcgccatg atcaccgacc ggatcgacgg cgacatcgcc cggtcgcgcg gcctggtgac
      841 tgatgtcggc aagatcctcg acccgatcgc cgacaaggcc ctgctgggga tggcgttcgt
      901 cggtctctcg ctcatcgggt tggtgccgtg gtgggtgacg ctcctcgtgc tcgcgcgcga
      961 ggtcggcgtc accgtcgtgc ggttcgtcgt tatccggcac ggcgtcatgc cggcagggcg
     1021 cgggggcaag atcaagacgt tcgcgcaggc cgttgcgctg tcgttgttga tcctgcccgc
     1081 gtgggtgctg ccagctccac agatctggca gtggacggca tacagcgtgt tggcgatcgc
     1141 cgtcgcgctc acggtggtga cgggtctgga ctacctcgtg caggcgcacc ggctccggcg
     1201 caccagcccc cgcgccatgg ccaagcgggc cgcgcgcgag gccaagaagc gatgagggcg
     1261 acccaggtcc tcgccgatct ggccgtgcgc ggcgagacga tcgcgtgcgc cgagtcgctc
     1321 accggaggcg cgctcacggc ccggctcatc gacgtccccg gtgcttcgcg tgtggtgcgc
     1381 ggcggggtgg tcgcctatgc agcggacgtc aaggccgcgg tgctcggtgt ctcggcggcg
     1441 cggctggcgt cgcacgggac ggtcgacccg caggtcgcgg cggagatggc cctcggggta
     1501 tgccgtgtgc tcaacagcga ctggggggtc gccaccacgg gggtcgccgg gccggaaccg
     1561 tccgagggca aacgggtggg gacggcatac gtcgcggtcg tccgggcggg cggcgagccg
     1621 gtgatccacg agttgcacct cgctggcaat cgcgccaaga tccgggccgc cgtggtggac
     1681 gccgcgttgg agttcatcga cgcggcgttg gccgcggacg acgctcagta ggcgctcgac
     1741 tcgcgcgcca cccgagggct ttgcactggc gtcggggctc tcttcgcgta tcgtgggggg
     1801 cactgacgtt tgtgccagat ggggacagga ggcgaaatgg tgctcatgcg ccgcgaactc
     1861 ggcgatgtgc tgcgcgagga acgtcgagcc cagaagcgca gcctgcgtga cgtctcggcc
     1921 tccgctgccg tatcgctcgg ctatctcagc gagatcgagc gaggcgagaa ggaagcgtcc
     1981 tccgagttgc tcggagccat ctgtgaagcg ctcgggctgc cgttgtcggc gctgctgcgc
     2041 gagttgtcgg tgcgggtcgc ccaggccgag ggtttcgcga ctcctgtcgt gctgccggtg
     2101 ccgtcgctcg gcgacgtctc cgcctccgcc gcctgaccgg cgggcaacac ctccgtggcg
     2161 cggattcgcg cggtccgcag cagcgtgctg tcgattccac tgcacacgcc cttcgtcacc
     2221 gcgctgcggc gcacgagcac gatcgacacg ctcatcgtgt gtctagacct cgctgacggc
     2281 accaccggcc tcggtgaagc gccacaggtg tgggcggtga ccggcgactc ggtcgcgagt
     2341 gccactgcct gtgtcgagca ggtcctcgcg ccggtgctcg tcggtgcgga cagtgatgac
     2401 tgggatgcgc ttgcggcggg ggtggcccgg gcgtgcgccc gcaacttcgg ggccaaggcc
     2461 gccgtcgaga ccgccttgct cgacgcggtc gcccgcgcag ccggtatgcc gctcgcttac
     2521 cttttcgccg acgttgccgg ggtgccgcgg ccggtctccc gcggagttgg tctgggtggc
     2581 ggggctgctg gggtgttcga ggtggcgacc gacgtgacca tctcggccgg gtcaccagag
     2641 gcgctgcgca gcgctgcgca gacccgggtc gcagagggtt tcacccgcct caaactcaag
     2701 gtcggcaccg acgccgcgac tgacgtcgca cgggtgcgtg ctgtccgtga ggcggcgggg
     2761 ccaggcatcg gggttcgact ggacgccaac caagggtggt cgcgcgaaga tgcggttgcg
     2821 gtgatccggg cactggccga ggcagatctg ggcgtcgaga tcgtcgagca gccggtcgcc
     2881 ggcgacgatg tcgaggggct ggcgtgggtg cgtgagcgcg tggacctgcc gatcatggcc
     2941 gacgagacgg tctatggagc caccgacctc gagagggtca ttcgtctgga ggccgccgat
     3001 gcggtcaacg tcaagctcgc caagtgcggg ggaccgctga cggggcttcg actgcttgcc
     3061 cgggcgcgcg aggctgggct ggggacgatg gtcggctcga tgatggagtc gcacgtcgga
     3121 gtggccacgg ctgcatccat ggttgccgcg gtcggcacca gcgcggtcag cgacctggac
     3181 gccgcgtggt gggccaccca ctcgccgtat gccggtgggc ccacctacgg tcccggcgtc
     3241 attcagctcc ccgacgccct aggcctaggg atgtccctgg cctagtgccg tgggccttca
     3301 aacgaccccg cggcaccacc ttcgaatcat cggcgaggcg gccctttacc atgccggacc
     3361 tggcgaccaa ccgaccccac gggcagggcc aactagtcgg gaaagggtgg tgaggatcgt
     3421 tacccgactg tgatgtattc gcctgtggga gaggcctttt ggggcggggg gtctgtcagt
     3481 ggtcgctgtt agcgtcgggg ttatggacag cagcgaagca actcgggact cggcatctcg
     3541 ccgggctgag atccgtgctg cgacgaggac tgccactgag ttggtggcgt cgttgtggca
     3601 ggtccaggga gccgagttag ggtcgttgtt gggtgagttg gacgctctcg ctgcggtcgc
     3661 gtgtggtgct cgggtcgcgg tcgttggtga agccg
//
LOCUS       DAOMZY010000340         3691 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_23939_length_3691_cov_3.863861, whole genome shotgun sequence.
ACCESSION   DAOMZY010000340 DAOMZY010000000
VERSION     DAOMZY010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23939_length_3691_cov_3.863861"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLK64_14910"
     CDS             complement(<1..180)
                     /locus_tag="PLK64_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HPV81083.1"
                     /translation="MSASYGELIAALESLPANPRIVASGNGAIPFELLHIVDNTLPAF
                     TLNMLAAHAGLPLREG"
     gene            270..>1264
                     /locus_tag="PLK64_14915"
     CDS             270..>1264
                     /locus_tag="PLK64_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPV81084.1"
                     /translation="MPASLRRLDPLLGDAILRDPDIAATYAVDSSYGASAPETFTVVR
                     ARDTQDVVAVLEEAQAHGIPVVPQGARTSLTGASCATEGAIVLNVEALRGIDIDPVEG
                     HARVGPGVVNADLKAAVREAGLFYPPDPASADTCTIGGNVATNAGGLCCIKYGVTADY
                     VKSLEIVLPGGEQIRTGKLTAKSTAGYDLTGLFVGSEGTLGVVTSALVRLIPAPDLAL
                     TALATFDSLDEAARAVLALRADRHRPALLEFLDRASIAAIQAMNDFGFPSGCEAALLV
                     QSDRPGHAAEDVERYAATLADLGAAEIAVADNAAEAELLMKGRRMLNYALEAKGGR"
     assembly_gap    1265..1364
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1387..1920
                     /locus_tag="PLK64_14920"
     CDS             1387..1920
                     /locus_tag="PLK64_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase family protein"
                     /protein_id="HPV81085.1"
                     /translation="MQEIETRSGWLTRDQLRDARERLPILYVNLVPVRVDERSQVTQI
                     GLLLRASETGEIQRELVSGRVLYHERLRDAVLRHIERDLGPMALPQVPIAPQPFAVAE
                     YFPTPGVTPFHDPRQHAVALAYVVVLMGDCQPQGNALDLAWLTPAEASELATTPEMVG
                     GHGVLLRQALAHLGHPI"
     gene            1934..2308
                     /locus_tag="PLK64_14925"
     CDS             1934..2308
                     /locus_tag="PLK64_14925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HPV81086.1"
                     /translation="MTSQPAAPTPPPSTPSAADLAARIRATTFPRIRYHKGYDRYDVD
                     WLLRKVATELERGVAKPQLADELAKAEFSTTTVRSGYDERTVDDFVDEVVGALRALLG
                     PVPPQRTKSTPLALVRRLLKRP"
     gene            complement(2309..2785)
                     /locus_tag="PLK64_14930"
     CDS             complement(2309..2785)
                     /locus_tag="PLK64_14930"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV81087.1"
                     /translation="MSPTHDPVGSAVRPAGPDDLAAVTDCRRRWVAERTDASDPGFED
                     RMADWWVREADRRATWLAWAGEQPIGMAATVIFERMPAPGALTARWGYVSQVWVDAAY
                     RRRGVASTLMGAVIAWARREGLVRLVLNPSDESRPMYAALGFREASDLLRLDLSGR"
     gene            complement(2782..>3691)
                     /locus_tag="PLK64_14935"
     CDS             complement(2782..>3691)
                     /locus_tag="PLK64_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HPV81088.1"
                     /translation="RVDAFNIDLANELTRGGRRSGLTFAPEGGSERLRKVINKQVSEQ
                     DLVNTVAAAFASGWRQVKLYFMCGLPTETDEDVLQIASLAKQVIETGRQVSGRRDVRC
                     TVSIGGFVPKPHTPFQWAAQLGAEATDARLQLLRDTIRADRRYAAAIGFRYHDGQPGI
                     VEGLLSRGDRRVGAVIEAVWRDGGRFDGWSEHFSYERWMRAAETVFADSPVSVDWFTT
                     RERDRIEVLPWDHIDSGLDRDWLWDDWQDALGEHEVADCRWSGCFDCGVCPEMGTQIE
                     MGPTGRTLLPLAVLSPGRARLLAPSP"
BASE COUNT          555 a         1312 c         1196 g          528 t
ORIGIN      
        1 gccctcgcgc agcggcaggc cggcatgcgc agcgagcatg ttgagggtga acgctgggag
       61 tgtgttatcg acgatgtgca gcagttcaaa cggaatggcg ccgttgccgc tggcgacgat
      121 ccgtgggttg gctggaagcg attcgagtgc ggcgatcaac tcaccgtacg aggccgacat
      181 ggctcaacta tcccgcacct ggacactctc gtcagaccaa ggctgcaggg ctcgtgagcc
      241 acgtctcatc tgacgttagg gtcagaccca tgcctgcttc cctgcggcgc cttgaccccc
      301 tcctcgggga tgccatcctt cgcgaccccg acattgccgc gacatatgcc gtcgactcct
      361 cctacggtgc gagcgccccc gagaccttca cggtggtgcg ggcccgcgac acccaggatg
      421 tcgtcgccgt gctggaggag gcgcaggcgc acggcatacc cgtggtccct cagggagccc
      481 gcacgtcgct caccggagcc tcctgcgcga ccgagggcgc catcgtcctc aacgtggaag
      541 ccttgcgggg catcgacatc gaccccgtcg aggggcatgc ccgcgtcggt cccggcgtcg
      601 tcaatgccga cctcaaggca gcggttcgcg aggcgggcct cttctatccg ccggacccgg
      661 cgtcagcgga cacctgcacg atcggcggca acgtggccac caacgcaggc ggcctgtgct
      721 gcatcaagta cggcgtgacc gccgactacg tcaagtcgtt ggagatcgtc ctccccggag
      781 gcgagcagat ccgcaccggg aagctcacgg cgaagagcac cgccggctat gacctcaccg
      841 gcctgttcgt cggctcggaa ggcaccctgg gcgtcgtcac gtcggcactg gtgcgcctga
      901 tcccggcgcc ggacctcgcg ctcacggccc tggcgacctt cgactcgctg gacgaagcgg
      961 cccgcgcagt cctcgcgctc cgcgctgatc gccatcgacc ggcgctgctg gagttcctcg
     1021 atcgcgcctc gatcgcggcc atccaggcga tgaacgactt cggcttcccc tcgggctgcg
     1081 aggcggccct gctcgtgcag tccgaccgcc cgggccacgc cgcggaggac gtcgagaggt
     1141 atgccgcgac gctagccgac ctgggcgcgg ctgagatcgc tgtcgcggac aacgccgccg
     1201 aggctgaact cctcatgaag ggccggcgga tgctcaacta cgccctggag gccaagggcg
     1261 gacgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgcgac tgacagaatc
     1381 gggaccatgc aggagatcga gacccgcagc ggatggctca cccgtgacca gctgcgcgac
     1441 gcgcgggaac ggttgccgat cctctacgtc aacctggtgc ccgtccgggt cgacgaacgc
     1501 agccaggtca cccagatcgg gctgctgctg cgcgccagcg agaccggcga gatccaacgg
     1561 gagctcgtct cgggacgggt gctctatcac gagcggctgc gcgatgcggt gctgcgccac
     1621 atcgagcgtg acctcggccc gatggcgttg ccgcaggtcc cgatcgctcc gcaaccgttc
     1681 gcggtcgctg agtacttccc cacgcccggt gtcacgccct tccacgaccc ccgccagcat
     1741 gccgtggcgc ttgcctacgt cgtcgtcctc atgggcgact gccagcccca aggcaacgcc
     1801 ctggacctgg cctggctcac ccctgcagag gccagcgagt tggctaccac gcccgagatg
     1861 gtcggcggtc atggggtgct gttgcgccag gcgctggctc acctcggcca tccgatctga
     1921 tcgtcgaccg cccgtgacga gccaacccgc tgcgcccact ccgcccccgt cgaccccgtc
     1981 ggctgcggac cttgcggccc ggatccgggc aacgaccttc ccccggatca ggtaccacaa
     2041 gggttacgac cgctacgacg tcgactggtt gctgcgcaag gtcgccaccg agctcgagcg
     2101 cggggtcgcc aagccgcagc tggccgacga actcgccaaa gcggagttca gcaccaccac
     2161 cgtccgctcc ggttacgacg agcggaccgt ggacgacttc gtcgacgagg tggtgggcgc
     2221 gttgcgcgcc ctgctcggcc cggtcccacc gcagcggacg aagtccacac cgctcgccct
     2281 cgtccggcgg ctgctcaaac gcccctgatc accggccgga cagatcgagc cgcagcaggt
     2341 cgctcgcctc gcggaagccg agcgcggcat acatcgggcg ggactcgtcg ctgggattga
     2401 ggacgaggcg cacgagacct tcgcgtcgtg cccaagcgat gaccgcaccc atgagggtgc
     2461 tcgccacccc acgtcgccgg tatgccgcgt cgacccacac ctgggacacg taaccccacc
     2521 gtgcggtcag cgcccccggt gcgggcatcc gctcgaagat gacggtcgcg gccatcccga
     2581 tgggctgctc ccccgcccag gccagccagg tggccctccg atcggcctca cggacccacc
     2641 agtcggccat ccggtcctcg aagccgggat ccgaggcgtc ggtgcgttcg gccacccagc
     2701 gccgacggca gtcagtgacc gcggcgaggt cgtccggccc ggccgggcgg acggcagacc
     2761 cgacggggtc gtgggtcggg ctcacggtga cggtgcgagc aaccgcgcgc ggcccgggct
     2821 gagcactgcc aacggcagca gcgtgcgacc ggtcggaccc atctcgatct gggtgcccat
     2881 ctcggggcag accccacagt cgaagcaccc actccaccgg cagtcggcga cctcgtgctc
     2941 ccccaacgcg tcctgccagt cgtcccacaa ccagtcgcgg tcgaggcccg agtcgatgtg
     3001 gtcccagggc aggacttcga tccggtcgcg ctcacgagtg gtgaaccagt cgaccgacac
     3061 cggcgagtcg gcgaagacgg tctccgcggc ccgcatccag cgctcgtacg agaagtgttc
     3121 gctccacccg tcgaatcggc cgccgtcgcg ccagacggcc tcgatgacgg caccgacgcg
     3181 gcggtcaccc cgggagagca ggccctcgac gatgcccggc tgcccgtcgt ggtagcggaa
     3241 gccgatcgct gcggcatacc ggcggtcggc gcggatggtg tcgcgcagca gttgcaggcg
     3301 ggcgtcggtc gcttcggcac cgagttgcgc cgcccattgg aagggggtgt gcggcttggg
     3361 cacgaacccg ccgatcgaca ccgtgcatcg gacgtcgcgg cgcccgctca cctggcgacc
     3421 ggtctcgatg acctgcttgg caagcgaggc gatctgcagt acgtcctcgt cggtctcggt
     3481 cggcagcccg cacatgaagt agagcttcac ctgccgccag cccgacgcga aggccgccgc
     3541 gaccgtgttg acgaggtcct gctcggagac ctgcttgttg atgaccttgc gcagccgctc
     3601 cgagccgccc tcgggcgcga aggtgagtcc cgaccggcgc cccccgcggg tcagctcgtt
     3661 ggccaggtcg atgttgaagg cgtccacccg g
//
LOCUS       DAOMZY010000341         3689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_23962_length_3689_cov_5.098789, whole genome shotgun sequence.
ACCESSION   DAOMZY010000341 DAOMZY010000000
VERSION     DAOMZY010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23962_length_3689_cov_5.098789"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1793)
                     /gene="pabB"
                     /locus_tag="PLK64_14940"
     CDS             complement(<1..1793)
                     /gene="pabB"
                     /locus_tag="PLK64_14940"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HPV81089.1"
                     /translation="MREAFVDFPWAPAGAAGPLAFGAPVEIVEARCLDEVRPALDRVA
                     AACADGRYAVGFVSYEAAPAFDPAMAVRSGSGVPLVWFGLHDAPLAEGAAREPAAYAC
                     SVWEAGTDEVTYAASVAAVREAIASGDTYQVNHTMRLRARFDGDPLGAWADLRRAQRD
                     GWFAYVDTGAHAVASVSPELFFTWDGHEVVTRPMKGTRARSRDGALDAARAADLRASA
                     KDRAENLMIVDLLRNDLGRVCRPGSVRVPSLFDVEAYPTVWQMTSTVTGESRPGLGLG
                     DLFGGLFPCGSITGAPKIRTMALITELEDRPRGVYCGAVGVVFPGGAAAFNVAIRTLV
                     VDQADGRARYDVGGGVVWDSDGRDEHAEALAKAAVLDMTQRDFALLETLRVETDEVVL
                     LDRHVERMARSAEWFGWPFDPDRARALVRSDGAGPARARLLLRPSGDLRVEFTSLTDI
                     FGMPPAPDRTAPPPRAVVLARSPVWSGDPWLRHKSTRRGVYERHRREATAYLQAAGWD
                     AGAPFDVLLWNERGELTELTIGNLMADVAGTLVTPPVACGLLPGTLRAHLLASGTVSE
                     RVLRVQELPSATALWLVNAVRGWVPITLVEPP"
     gene            1925..2305
                     /gene="hisI"
                     /locus_tag="PLK64_14945"
     CDS             1925..2305
                     /gene="hisI"
                     /locus_tag="PLK64_14945"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HPV81090.1"
                     /translation="MAAVSLRASSLDPAVAARLKRDDQGLVAAIVQQHDTGEVLMLGW
                     MDDEALARTLTTGRVTFWSRSRGEYWRKGDTSGHVQHVRSVAVDCDGDALLVRVDQVG
                     AACHTGDRTCFDARPLAVVGGHDE"
     gene            2298..>3689
                     /locus_tag="PLK64_14950"
     CDS             2298..>3689
                     /locus_tag="PLK64_14950"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HPV81091.1"
                     /translation="MSDIHAELTFGSTWPSLDVFGELARTRRVIPVVRRLLADGETPL
                     GVYRKLAKGEPGTFLLESAEHGGVWSRYSIVGAHSAATLTERDGQAHWIGMPPAGVPT
                     DGDPTIALRDTVAALATARIGGLPPLTGGMVGAITYDAVRRWEKVPDTGRDELNLPEL
                     AMVLATDLAVLDHSDGSVLLVANAINYDDTDERMEWAWRDAVARLDRMTAELGEPAPS
                     TVAVIEPVTLTVTSSHEPEAYLDNVERCKESIRAGDAFQIVVSQRFSTPCDADPIDVY
                     RALRAANPSPYMYLLRLPHPDGTAYDIVGSSPEALIKVTGREAITHPIAGSRPRGKTP
                     EDDVRLADDLAADTKERAEHVMLVDLSRNDLQRVCRAGTVDTVEFMTVRRYSHIMHLE
                     STVVGELKEGVAAYDVLVATFPAGTLSGAPKPRAMALIDEYEGLKRGIYGGVVGYLDF
                     AGDLDMAIAIRTAV"
BASE COUNT          584 a         1379 c         1237 g          489 t
ORIGIN      
        1 ggcggctcca ccagcgtgat cggcacccag ccacgcacgg cgttgaccag ccagagcgcc
       61 gtggcgctcg gcaactcctg cacccgaaga actcgctcgc tgaccgttcc tgacgccaac
      121 aggtgagctc gcaaggtgcc gggcagcagc ccgcacgcga ccggcggggt caccaaggtc
      181 ccggcgacat cggccatcag gttgccgatg gtcagctcgg tgagctcgcc gcgctcgttc
      241 cacagcagga cgtcgaacgg cgcccctgcg tcccatcccg ccgcctgcag gtatgccgtc
      301 gcctcgcgcc ggtggcgctc gtagacccct cgtcgggtgc tcttgtgccg caaccacgga
      361 tcaccggacc acaccgggct ccgagccagc accaccgccc gaggtggggg agcggtgcgg
      421 tcgggggcgg gcggcatacc gaaaatgtcc gtgagagagg tgaactcgac gcgaagatcg
      481 cccgaagggc gcagcagcag gcgagcccgg gccgggccgg caccgtccga acggaccaac
      541 gctcgcgctc ggtcggggtc gaagggccac ccgaaccact cggccgacct cgccatccgc
      601 tccacgtgcc gatcgagcag aaccacctcg tcggtctcga cccggagcgt ctccagaagg
      661 gcgaagtcgc gctgggtcat gtcgagcaca gcggccttgg ccagcgcctc agcgtgctcg
      721 tcgcgaccgt ccgagtccca gacgacaccg cctccgacgt cgtaacgcgc ccgaccgtcc
      781 gcctggtcga cgaccagcgt gcggatggcg acgttgaagg ccgccgcccc accgggaaag
      841 accacaccca ccgcgccgca atagacccca cgcggccgat cctcaagctc cgtgatgagc
      901 gccatcgtgc ggatcttcgg tgcccccgtg atcgatccgc aggggaagag accgccaaac
      961 aggtcgccca accccagccc cggacggctc tcgccggtca ccgtcgaggt catctgccac
     1021 acggttggat aggcctcgac gtcgaaaagg cttggcacgc ggaccgatcc gggcctgcag
     1081 acccgtccca ggtcgttgcg cagcaggtcg acgatcatga ggttctcggc ccggtccttg
     1141 gcggaggcgc gcaggtccgc ggctcgggcg gcgtccagcg ccccgtcgcg agatcgcgcg
     1201 cgggtgccct tcatcgggcg ggtgaccacc tcgtggccgt cccaggtgaa gaacagttcg
     1261 ggggacaccg aagcgaccgc atgggcaccc gtgtcgacgt aggcgaacca cccgtcgcgc
     1321 tgcgcccggc gcaggtccgc ccaggccccc agcggatccc cgtcgaagcg cgcccgcagc
     1381 cgcatcgtgt ggttcacctg ataggtgtca ccactggcga tggcctcgcg gacggcggcg
     1441 acggacgcgg catacgtcac ctcgtcggtg cccgcctccc acaccgagca cgcgtatgcc
     1501 gcgggctccc gtgccgcccc ctcggcgagg ggagcgtcgt gcaggccgaa ccagacgagg
     1561 ggcacgccgg acccgctgcg gaccgccatc gccggatcga acgcaggggc ggcctcatac
     1621 gacacgaacc cgacggcata ccgaccgtcc gcgcacgctg ccgcgacccg atccagcgcg
     1681 ggtcggacct cgtcgaggca gcgcgcctcg acgatctcga cgggcgcgcc gaacgccagc
     1741 ggccccgcag ccccggccgg ggcccaggga aagtcgacga acgcctccct cacggcgccc
     1801 aaggctaggg cacatcgacc ccgaccagag tgtggccgaa cgaggttcct gcgccgcgtt
     1861 ccactgcacc catcgctccg ccgccatggg acgccgccgc caccgcccca gcggagcggg
     1921 cagaatggca gccgtgtccc ttcgcgccag ctccctcgac ccggccgtcg ccgcccgcct
     1981 caagcgcgac gaccagggcc tcgtggcagc catcgtccag cagcacgaca ccggtgaggt
     2041 gctcatgctc gggtggatgg atgacgaggc cctcgctcgc acgctgacga cgggccgggt
     2101 gaccttctgg tcgcgctcgc gcggtgagta ctggcgcaag ggcgacacct ccgggcacgt
     2161 ccaacacgtc cgttccgtgg ccgtggactg cgacggcgac gccctgcttg tgcgcgtcga
     2221 ccaggtgggc gcggcctgcc acaccggcga ccgcacctgc ttcgacgcgc ggccgctggc
     2281 cgtggtggga ggccacgatg agtgacatcc acgccgagtt gaccttcggc agcacctggc
     2341 cgagcctgga cgtcttcgga gagttggctc gcacccgtcg ggtcatcccc gtcgtgcggc
     2401 ggctgctggc cgacggtgag acgccgctcg gggtctaccg caagctggcc aagggcgagc
     2461 cgggcacgtt cctgctcgag tcggccgagc acggtggggt ctggtcgcgc tactcgatcg
     2521 tcggggccca cagcgcggcg accctcaccg agcgggacgg ccaggctcac tggatcggta
     2581 tgccgcccgc cggggtcccg accgacggcg acccgacgat cgccttgcgc gacaccgtcg
     2641 ccgccctcgc gaccgcgagg atcggcggtc tgcccccgct caccggcggg atggtcgggg
     2701 ccatcaccta cgacgcggtc cggcggtggg agaaggtgcc cgacaccggc cgcgacgagt
     2761 tgaacctccc cgaactggcc atggtgctgg ccaccgatct cgcggtcctc gaccactccg
     2821 acggctcggt gctgctcgtc gccaacgcga tcaactacga cgacactgac gagcggatgg
     2881 agtgggcctg gcgtgatgcg gtggctcggc tcgaccggat gaccgccgaa ctcggtgagc
     2941 cggcgccctc gacggtcgct gtgatcgagc cggtgaccct caccgtcacc agttcccacg
     3001 agccggaggc ctacctcgac aacgtcgagc ggtgcaagga gtcgatccgc gccggcgatg
     3061 ccttccagat cgttgtctcg caacggttct cgacgccgtg cgacgccgac cccatcgacg
     3121 tctaccgggc actgcgggcc gccaacccca gcccctacat gtacctgctg cgcctgccgc
     3181 acccggacgg cacggcatac gacatcgtgg ggtccagccc ggaggccctc atcaaggtga
     3241 ccgggcgcga ggcgatcacc cacccgatcg ccggttcgcg cccccgtggc aagacccccg
     3301 aggacgacgt ccgcctggcc gacgatcttg ccgccgacac caaggaacgc gccgagcacg
     3361 tcatgctcgt cgacctctcg cgcaacgacc tccagcgggt atgccgcgcg ggcaccgtcg
     3421 acacggtcga gttcatgacg gtccgccgct acagccacat catgcatctg gagtccacgg
     3481 tcgtcggcga gctcaaggag ggcgtggcgg catacgacgt gctcgtggcg accttccctg
     3541 cggggaccct ttcgggagca cccaagccac gtgcgatggc cctcatcgac gagtacgaag
     3601 ggctcaagcg ggggatctac gggggagtgg tgggttatct cgacttcgcc ggtgacctcg
     3661 acatggcgat cgcgatccgc accgccgtc
//
LOCUS       DAOMZY010000342         3675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_24088_length_3675_cov_6.552210, whole genome shotgun sequence.
ACCESSION   DAOMZY010000342 DAOMZY010000000
VERSION     DAOMZY010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24088_length_3675_cov_6.552210"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="PLK64_14955"
     CDS             <1..1147
                     /locus_tag="PLK64_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81092.1"
                     /translation="VLTGRVVRPVDVLPEYLLAATPLEADVALALASVTSGGRHPFGF
                     VGAGQVPGAAWVAHVDEAGTLTLPLLDIEVSAHRVVPRTLDALAELFMAASQPQAGPP
                     AGAAAAPGAPAVALAPPPAVVQDDALLRTAAASVSLLGPLTLSAPGRIDPSQIPLAEE
                     IVAYLAVNPGGVHSGVLAAAIWPRGVGQAVASATIDRVRDWLGTDADGNYRLMLDPSG
                     RLRLSDAVGVDWFAFCTLTQRAGAARSAQEESELLRRALRLVRGQMLEASAPGRFSWL
                     ARTTLTRTVEQSVVSVAHRLVVLAEREPDPEGAAVAAALGLRMAPLSQLLWRDLIAAQ
                     HAGHGSEAMKGAVAEMVRVLDGADVPMEGETEALIAHLSGPGSAAQA"
     gene            1219..2529
                     /locus_tag="PLK64_14960"
     CDS             1219..2529
                     /locus_tag="PLK64_14960"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HPV81093.1"
                     /translation="MTTPASAAPEPFDAVDVIVTKRNKGDLSDGQIDWVIDAYTRGVV
                     ADEQMSSLAMAILLNGMTRREIARWTGAMIASGERMDFSSLSRPTADKHSTGGVGDMI
                     TLPLAPLVAACGVAVPQLSGRGLGHTGGTLDKLESIPGWRASVSNEQMLRQLEEVGAV
                     ICAAGAGLAPADKKLYALRDVTGTVEAIPLIASSIMSKKIAEGTGALVLDVKVGSGAF
                     MKDVAQAQELARTMVALGTDAGVKTVAMLTNMQTPLGLTAGNALEVRQSVEVLAGGGP
                     PDVVDLTLTLAREMLAAAGREEIDPADALADGRAMDVWHRMIQAQGGDSHALLPWARE
                     TYEVRAPGTGVLTQLDALKVGVAAWRLGAGRARKEDPVQAGAGVVMHAKPGAYVRAGD
                     PLLTLHTDTPERFERALEALEGAWFIAPEGSRPDLLPLVIDRIS"
     gene            2588..2941
                     /locus_tag="PLK64_14965"
     CDS             2588..2941
                     /locus_tag="PLK64_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPV81094.1"
                     /translation="MPHVTTAPTRIPVPGGKVIDEHVGAASTGTDAVSVAHMVAPPGW
                     EEPYQTPQFDEITVVVRGSIHVDCDGVRHEVHAGQSIVTTAGERIRYAAGPDGAEYVA
                     VCLPAFTPDRVHRED"
     gene            3007..>3675
                     /locus_tag="PLK64_14970"
     CDS             3007..>3675
                     /locus_tag="PLK64_14970"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase family protein"
                     /protein_id="HPV81095.1"
                     /translation="MIAAPKVLLHDHLDGGARSQTVIEIAEQIGYADLPSTDVAELDQ
                     WFRDSADSGSLVRYLQTFDQTVAVMQTEEGLHRIAKECVLDLATDGVVYAESRYAPEQ
                     HLAEGLTLEQVVESVNAGFRDGEREAAAAGHPIQVTALLTAMRHAAKSSEIARLAVRY
                     RDDGVAGFDIAGAEDGHPPTRHLDAFEYLQRENAHFTIHAGEAFGLPSIWEAIQYCSA
                     DRLGH"
BASE COUNT          522 a         1313 c         1300 g          540 t
ORIGIN      
        1 cgtgctcacc ggccgggtcg ttcggcccgt ggacgtgctg ccggagtatc tcttggcggc
       61 gaccccgctt gaggccgatg tggcgctcgc gctggcgtcg gtcacgtcgg gaggtcggca
      121 cccgttcggt ttcgtcggcg ccggtcaggt gcccggcgcc gcgtgggtcg cccatgtcga
      181 tgaggccggg accttgacgt tgcccctgct ggacatcgag gtcagcgccc accgggtggt
      241 gccgcggacg ctcgacgcgt tggcggagtt gttcatggct gcctcgcagc cccaggccgg
      301 tccgcccgcg ggcgctgcgg ccgcgccggg ggctcccgcc gtggcactcg cgccccctcc
      361 tgccgtggtg caggacgacg ccctgctgcg caccgccgcc gcgtcggtca gcctgctcgg
      421 cccgctcacg ttgtctgcac ccggccggat cgacccgagc cagatcccgt tggccgagga
      481 gatcgttgcc tacctggcgg tgaatcccgg cggggtccac agcggtgtcc tggcggcggc
      541 gatctggccc cgcggggtcg gccaggcggt cgcgtcggca acgatcgacc gcgtgcgcga
      601 ctggctgggc accgacgcgg acggcaacta tcggctcatg ctcgacccgt ccgggcggct
      661 gcggctctcg gacgccgtcg gcgtcgactg gtttgcgttc tgcaccctca cgcagcgggc
      721 cggtgcggct cgatccgcgc aagaggaatc cgagttgctg cgtcgggctc tgcgcctcgt
      781 ccgcggccag atgctcgaag cgagtgcacc ggggcggttc tcgtggctgg cccgcacgac
      841 gctcacccgc accgtggagc agtccgtcgt ctcggtggcg caccggcttg ttgtcctggc
      901 cgagcgcgag cccgaccccg agggagcggc cgtcgcggct gcgctcgggt tgcggatggc
      961 gccgctgtcc caactgctgt ggcgtgacct gatcgccgcc caacacgccg ggcacggcag
     1021 cgaggccatg aagggcgcgg ttgccgagat ggtccgggtc ttggacggtg cggacgtccc
     1081 gatggagggc gagaccgagg ccctcatcgc ccacctctcc gggcctgggt cggccgcgca
     1141 ggcctgaccg cgcgcgtccg ctcacggcat accggctaga cctcgtatgc cgcgtgatgg
     1201 tgagccatga tgggcgccat gacgaccccc gccagcgcag cccccgagcc gttcgacgcc
     1261 gtcgatgtca tcgtcaccaa acgcaacaag ggcgacttga gcgacgggca gatcgactgg
     1321 gtcatcgacg cttacacgcg gggcgttgtc gccgatgagc agatgtcctc gctcgcgatg
     1381 gcgatcctgc tcaacgggat gacccgccgc gagatcgccc ggtggacggg ggcgatgatc
     1441 gcctcaggcg agcggatgga cttctcgtcg ctgtcgcgtc ccactgcgga caagcactcc
     1501 accggcgggg tcggcgacat gatcacgctg cccctggcac ccctcgtcgc ggcgtgcgga
     1561 gtggccgtgc cgcaactgtc cggccgcggt ctcggccaca ccggtggcac cctcgacaag
     1621 cttgagtcca tcccggggtg gcgcgcctcg gtgagcaacg agcagatgct gcgccaactc
     1681 gaggaggtcg gcgcggtcat ctgcgcggcg ggcgccggcc tcgcaccggc cgacaagaag
     1741 ctttatgccc tccgcgatgt cacgggcacc gtcgaggcga tcccgctcat cgccagctcg
     1801 atcatgagca agaagatcgc cgaggggacc ggggcgctcg tgctcgacgt caaggtcggc
     1861 tccggtgcct tcatgaagga cgtagcccag gcccaggaac tggcccggac catggtggcg
     1921 ctggggaccg acgctggggt caagacggtt gcgatgctca ccaacatgca gaccccgctg
     1981 ggcctcaccg cgggcaacgc ccttgaggtg cgtcagtcgg tcgaggtgct cgccggcggt
     2041 ggtcctccgg acgtcgtcga cctgaccctc acgctggccc gggagatgct ggcagccgcc
     2101 ggccgcgagg agatcgaccc ggccgacgcg ttggcggacg ggcgcgcgat ggacgtgtgg
     2161 caccggatga tccaggcgca gggcggcgac tcgcacgcgc tgctgccgtg ggcgcgggag
     2221 acctatgagg tgcgggcgcc cggcacgggg gtgctgaccc aactcgacgc cctcaaggtc
     2281 ggggtggccg cctggcggct cggtgctggc cgggctcgca aggaagaccc ggtgcaggcc
     2341 ggggccggtg tcgtcatgca cgccaaaccg ggtgcctacg tccgggccgg cgacccgctg
     2401 ctcaccctgc acaccgacac ccccgaacgc ttcgagcgcg cgctggaggc gttggagggt
     2461 gcgtggttca tcgcaccgga agggtcccgt cccgacctgc tgcccctcgt catcgaccgg
     2521 atcagctgac ccgcggaccg ggccacacgg catacgcggg ggctcgctaa ccctctaggc
     2581 tgcgaggatg ccgcacgtca ccaccgcgcc gacgcggatc ccggtccctg ggggcaaggt
     2641 catcgacgaa cacgtgggag ccgcctcgac cggcaccgac gccgtgtcag tcgctcacat
     2701 ggtcgccccg cccgggtggg aagagcctta ccagacacca caattcgacg aaatcaccgt
     2761 cgtcgtcagg ggctcgatcc acgtggactg cgacggcgtc cgtcacgagg tccacgccgg
     2821 acagtcgatc gtcacgaccg caggcgaacg catccggtat gccgccggcc ccgacggcgc
     2881 agagtatgtc gccgtctgcc tgcccgcctt cacccccgac cgtgtccacc gagaggactg
     2941 accgtgagcc cgcggcctga cccgagcccg agcccgagcc cgcgctccgg tcctgatctc
     3001 gcgacgatca tcgcggcccc gaaggtgctc ctgcacgacc acctcgacgg gggagcgcgc
     3061 tcccagacgg tcatcgagat cgccgaacag atcgggtatg ccgacctgcc gagcaccgac
     3121 gtcgccgagt tggaccagtg gttccgcgac agcgccgact cgggctcgct cgtgcgctat
     3181 ctgcagacct tcgaccagac ggtggcggtc atgcagaccg aggaagggct gcaccggatc
     3241 gccaaggagt gcgtcctcga cctcgccacc gacggcgtcg tctatgccga gagtcgctat
     3301 gcccccgagc agcacctggc cgagggcctg accctcgagc aggtcgtcga gtcggtcaac
     3361 gctggcttcc gggacggcga gcgcgaggcc gcggcagccg gtcacccgat ccaggtcacg
     3421 gcgctgctca cggcgatgcg ccatgcggcc aagagctcgg agatcgcccg gttggccgtg
     3481 cgttaccgcg acgacggggt ggccggattc gacatcgccg gggccgagga cggtcacccg
     3541 ccgacccgcc acctggacgc gttcgaatac ctgcagcgcg agaacgccca cttcaccatc
     3601 cacgcgggcg aggccttcgg gttgccgagc atctgggagg ccatccagta ctgctcggct
     3661 gatcggctcg gccac
//
LOCUS       DAOMZY010000343         3674 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_24100_length_3674_cov_4.169936, whole genome shotgun sequence.
ACCESSION   DAOMZY010000343 DAOMZY010000000
VERSION     DAOMZY010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24100_length_3674_cov_4.169936"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PLK64_14975"
     CDS             complement(<1..268)
                     /locus_tag="PLK64_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease
                     domain-containing protein"
                     /protein_id="HPV81096.1"
                     /translation="MSHADAGMPRPRTALPGARSGLLTEVLIVLGLSLGASAIWSVLR
                     IVERLTRAVPLGQQTSSLNTSVTPDRPWLDLAYQLTGIGLALMPV"
     gene            314..931
                     /locus_tag="PLK64_14980"
     CDS             314..931
                     /locus_tag="PLK64_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81097.1"
                     /translation="MRQRVRFEGQIAGVGSTSGVRVVIGHWVRSPFGAFADAMVERAD
                     GWRILIVPSERARELVCGTYVFDESRVEPVSLVEHSGGAEWEFTSPSLHLRLGIGPRL
                     PLGRLLRMVPPPVATSPGWASLVDPVARIVLRGVRTRGTAGQGRREFYGATDLRSVLS
                     AEGELDGIPLGELAPVDPPCRFGFSSTPRQPSVTTVVTTIESVLA"
     gene            928..1800
                     /gene="rsmI"
                     /locus_tag="PLK64_14985"
     CDS             928..1800
                     /gene="rsmI"
                     /locus_tag="PLK64_14985"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPV81098.1"
                     /translation="MTGVLVLAATPIGDPRDAAPRLGEELANADVVAAEDTRRLRRLT
                     TDLGVSIAGRVLSYHEHNEAARTPELVEQLLSGARVLVVTDAGMPSVSDPGYRLVAAA
                     VEAGVRVTCVPGPSAVLMALAVSGLPVDRFCFEGFLPRKPGERARALTALADEPRTMV
                     FFEAPHRTAATLAAMAAVWGVERPGAVCRELTKTYEEVRRGGLGELAEWAEAGVLGEV
                     TLVVAGRPARAPFEAGGAEFAAVVHGIQERVAAGERLKDVCADVAAATGLAKRTLYDA
                     AVSSRGGTAPRPPA"
     gene            complement(1760..2233)
                     /locus_tag="PLK64_14990"
     CDS             complement(1760..2233)
                     /locus_tag="PLK64_14990"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPV81099.1"
                     /translation="MADVRETLHMDAPAELVYDLVADLPRMGEWSTECERVVWRGTAT
                     KAEHGAGFIGHNRAGTVRWITFGKVTAAERGRYLAFDIFVGPIQVSRWEYVFAPDEDG
                     QGCTVAEQWTDRRSAVQRTLGNRVVGERVEKNREGIRQTLRTLKRVAEAQFHPAS"
     gene            2312..2815
                     /locus_tag="PLK64_14995"
     CDS             2312..2815
                     /locus_tag="PLK64_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPV81100.1"
                     /translation="MTAPEIPQLREPANLVCPRARWLWLAGGVGEAVLLAALQVGWWF
                     IDQRGSRTPHIIVAVVLGVLVVAYLVVMPIWRYRVHRWEVTSSAVYTQTGWISQERRI
                     APLSRVQTVDLTRGPIAQLLRLATVRVTTASAAGHLVVHGLDQDEAAALVERLTAAAA
                     AERGDAT"
     gene            2812..>3674
                     /locus_tag="PLK64_15000"
     CDS             2812..>3674
                     /locus_tag="PLK64_15000"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPV81101.1"
                     /translation="MSDGIPPILAGDPQWRRLDARMLLVHPVQEAVRFLPALLVVFLS
                     GRSSDRSQWWDLGILAIIVVLGIMRWVTTRYRIWGGQIELRQGLVNRRLLTTPADRVR
                     TVDVTAPIWHRALGLARVEIGTAGGGLGAHRIVLDALAAPVAAQLRGELLHRVATADG
                     PADVPADAPDSPDAEPRVSVGPAVDEPEEVLVRLDPAWVRYAPLTTSGLVSAAAIWGV
                     SAQYIGQVDELKDRVEGAVDRVAALGVGVAALAGFLSALIAVSVLAVSGYVLTYWGFR
                     LTRHTGGTLHI"
BASE COUNT          461 a         1255 c         1364 g          594 t
ORIGIN      
        1 cgaccggcat gagggccagg ccgatgccgg tcagttggta ggccaggtcc agccagggcc
       61 ggtccggcgt gaccgacgtg ttgagcgagg aggtctgctg gcccagaggg accgcacggg
      121 tgagccgttc gacgatccgc agcaccgacc agatcgccga cgcgccgagc gagagcccga
      181 gcacgatgag cacttcggtc agcaggccgc tgcgcgcgcc gggcagggcg gtgcgcggac
      241 gcggcatacc tgcgtcggcg tggctcatga gatcggacct tagccgcccc cgctgagggg
      301 ggaggatgca cgggtgaggc aacgtgtgcg gttcgagggg cagatcgccg gggtcggctc
      361 gacgagtggg gtccgggtcg tgatcggaca ttgggttcgc agccccttcg gcgccttcgc
      421 cgatgccatg gtcgagcggg ccgacggctg gcggatcctc atcgtcccct ccgagcgggc
      481 gcgtgagttg gtctgtggca cctatgtgtt cgacgagagc cgcgtggagc cggtgagcct
      541 ggtcgagcat tccggcggcg ccgagtggga gttcacgtcg ccctccctgc acctgcggct
      601 cgggatcggc ccgcgactcc cgctcggtcg gctgctgcgg atggtcccgc cccccgtggc
      661 cacgagcccc gggtgggcgt cgctggtcga cccggtggcg cgaatcgtgt tgcgcggcgt
      721 ccggacccgc gggacagccg gtcaaggccg ccgggagttc tacggcgcta ccgacctgcg
      781 ctcggtcctg agcgcggaag gtgaattgga cggcataccc ctgggggagt tggccccggt
      841 cgacccgccg tgccggttcg ggttctcttc caccccacgg caacccagcg tcacgaccgt
      901 cgtgacgacg atcgagtcgg tgctcgcatg accggcgtcc tcgtcctggc tgcgacgccg
      961 atcggcgacc cacgggacgc cgccccccgg ctgggcgaag aactcgcgaa cgccgacgtg
     1021 gtcgcggcgg aagacacccg ccgactgcgg cggctcacga ccgacctcgg ggtgagcatc
     1081 gccggccggg tgctcagcta tcacgagcac aacgaggctg cccgcactcc cgagctggtc
     1141 gagcagttgc tctctggcgc aagggttctc gtggtcacag acgccggtat gccgtccgtc
     1201 tcggaccctg gctaccgact tgttgccgct gcggtcgagg ctggcgtccg ggtcacctgt
     1261 gtccccggcc cgagcgccgt gctcatggcg ctcgcggtca gcgggctccc ggtcgaccgc
     1321 ttctgcttcg agggtttcct gccgcgcaaa ccgggagagc gggcccgggc gctgactgcg
     1381 ctcgcggacg agccgcggac catggtcttc ttcgaggcac ctcaccgcac cgccgcgacg
     1441 ctcgctgcga tggctgcggt ctggggggtc gaacgtcccg gtgccgtatg ccgcgagctc
     1501 accaagacct acgaagaggt ccgccgcggc gggctgggtg agctcgccga gtgggccgag
     1561 gccggggtcc tcggcgaggt gacccttgtc gtcgcgggcc gaccggcccg agccccgttt
     1621 gaggcgggcg gtgcggagtt cgcggcggtg gtgcacggca tacaggagcg ggttgccgcg
     1681 ggggagcggc tcaaggacgt ctgcgccgac gtcgcggcag cgaccggtct ggcgaagcgg
     1741 acgctctacg acgcggcggt cagctcgcgg ggtggaactg cgcctcggcc acccgcttga
     1801 gggttcgcag ggtctggcgg atgccctcgc ggttcttctc gacgcgctcg ccgacgaccc
     1861 ggttgcccag cgtccgctgg acggccgagc gccggtcggt ccactgctcg gccaccgtgc
     1921 agccctgacc gtcctcgtcg ggggcgaaga cgtattccca tcgcgacacc tggatcggcc
     1981 cgacgaagat gtcgaaggcg aggtagcggc cccgctcggc ggcggtcacc ttgccgaagg
     2041 tgatccaccg aaccgtgccg gcccggttgt gcccgatgaa gccggccccg tgctcggcct
     2101 tcgtcgctgt gccacgccag acgacccgtt cacactcggt ggaccactca cccatccggg
     2161 gcaggtcggc gacgaggtcg tagaccagct cggcgggcgc gtccatgtgc agggtctcgc
     2221 gcacgtcggc catgggacca tcctgtccca gccgcctgtg tgccgccgct tcggggtgcc
     2281 gtgtgtcggg cacgtgtgat ctgctggaca catgaccgcc ccggagattc cgcagttgcg
     2341 cgagcccgca aacctcgtgt gcccccgcgc gcggtggttg tggctggcgg gcggtgtggg
     2401 ggaggccgtg ctgctggccg cccttcaggt ggggtggtgg ttcatcgacc aacgtggctc
     2461 ccgaaccccg cacatcatcg tcgccgtggt gctcggggtg ctcgttgtcg cctacctcgt
     2521 cgtcatgccg atctggcggt atcgggtcca tcggtgggaa gtcacgtcga gcgccgtcta
     2581 tacccagacc ggttggatca gccaggagcg gaggatcgcc ccgctctccc gtgtgcagac
     2641 cgtcgacctc acccgggggc caatcgccca actgttgcgg ctggcaaccg tgcgggtcac
     2701 caccgcctcc gccgccggcc acctggtcgt ccacggcctg gatcaggacg aggcagcggc
     2761 cctggttgag cggttgaccg ccgcagcggc cgccgagcgc ggggacgcga cgtgagcgac
     2821 ggcatacctc cgatccttgc tggcgacccg cagtggcgcc gcctcgatgc gcggatgctc
     2881 cttgtccacc cggtgcagga ggcggtgcgg ttcctcccgg ccctgctggt ggtcttcctg
     2941 tccgggcgct ccagcgatcg cagccagtgg tgggacctcg ggatcctcgc catcatcgtg
     3001 gtgctgggca tcatgcgctg ggtcacgacc cgatatcgga tctggggtgg ccagatcgag
     3061 ttgcgccagg ggctggtcaa ccggcggctg ctgacgactc cggccgatcg agtccgcacg
     3121 gttgacgtca cggcgccgat ctggcaccgg gctcttgggc tggcccgggt ggagatcgga
     3181 accgcgggcg gtgggttggg cgctcatcgg atcgtcctcg acgccctggc cgcgccggtg
     3241 gcggcgcagc tgcgcgggga gttgctgcac cgggtggcga ccgccgacgg gccggccgac
     3301 gtgccggccg acgcgcccga ctcgcccgac gccgagccga gagtctcggt agggccggcg
     3361 gtcgacgagc ccgaagaggt cctcgtcagg ctggacccgg cgtgggtccg ctacgccccc
     3421 ttgacgacca gtgggctggt gtcggcagcc gccatctggg gcgtctccgc gcaatacatc
     3481 ggccaggtcg atgaactcaa ggatcgggtc gagggtgccg tcgaccgggt cgccgcgctc
     3541 ggcgtcgggg tggcggcgct cgccggtttc ctttcggcgc tgatcgccgt gtcggttctg
     3601 gccgtgtcgg ggtatgtctt gacctactgg gggttccggc tcacgcggca taccggcggc
     3661 accttgcaca tcag
//
LOCUS       DAOMZY010000344         3673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_24107_length_3673_cov_5.573521, whole genome shotgun sequence.
ACCESSION   DAOMZY010000344 DAOMZY010000000
VERSION     DAOMZY010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24107_length_3673_cov_5.573521"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..1360
                     /gene="pdhA"
                     /locus_tag="PLK64_15005"
     CDS             260..1360
                     /gene="pdhA"
                     /locus_tag="PLK64_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HPV81102.1"
                     /translation="MIQVLTPEGERVTSAPREYLEIIDALTDEDLRGFYRDLVMIRRF
                     DAEATALQRQGELGLWASLLGQEAAQVGSGRALRPQDYAFPTYREHGVAWCRGVDPVN
                     LMGMFRGVNHGGWDPNEKNFHLYTIIIGAQTLHATGYAMGVQRDGAVGTGDDERDTAV
                     IAYFGDGATSQGDVSESLIFAGVNNSPVVFFCQNNQWAISEPNEKQTRAPLYHRARGF
                     GFPGLRVDGNDVLAVYAVTKVALDAARSGQGPSFIEAFTYRMGAHTTSDDPTKYRMSA
                     EVEVWKLRDPIERLKAWLAHEGKADGAFFAQVAAEADELGADVRARCLALPDPAGTDM
                     FDHVYGERHPVMERERAEFAAYQAGFEGANHG"
     gene            1353..2363
                     /locus_tag="PLK64_15010"
     CDS             1353..2363
                     /locus_tag="PLK64_15010"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HPV81103.1"
                     /translation="MAERTRTDGPAAYPVTTTLAKGITSGLRAAMEHDPKVVLMGEDI
                     GKLGGVFRVTEGLQKDFGEDRVIDTPLAEAGIMGTAVGLALRGYKPVVEIQFDGFVYN
                     AFDHMVSSIAKMRARSLGHVGMPIVVRIPFGGGIGAVEHHSESNEAYFAHTAGLRVVA
                     CSNPHDAHWMIQQAIACPDPVIFYEPKRRYWDKGEIAAEPGRGLFQAATLRQGSDVTV
                     LAYGPMVRTCLQAAQAAAEEGRSLEVIDLRSLSPLDTGAIIESVRRTGRCVVVHEAST
                     FLGLGAEIAALIQQECFYSLQAPVARVGGYNVPYPPSRIEEEFLPDLDRVLDAVDRLM
                     AY"
     gene            2377..>3673
                     /locus_tag="PLK64_15015"
     CDS             2377..>3673
                     /locus_tag="PLK64_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HPV81104.1"
                     /translation="MSVREFLLPDPGEGLTEAEIVTWRVAVGDVVKVNDIVVEIETAK
                     SLVELPIPWAGTVAALLAPEGATVEVGSAIIAIDDPLYAGPPPSASGPAAASSSLTAM
                     AGNLVGGADSSTPSTSLGAMAAKLDGGGAGAGPATGGTSGGTTGGTSGGADEAVAEGL
                     IGGTTSTGRTAVLVGYGIKQTEAVRRPRRKDELARTVARPPDKEGGHIEQPPSAHMGG
                     LVAAHAPEVRRDTRMLTELAPVVAPLAPAPGAPVLAKPPVRKYAKDLGVDLAQVPTPT
                     GIVTRADIDAYIHGGAPDETVTAAAYASGMPSRADARGETRVPIKGVRKMTAAAMVGS
                     AFTAPHVTEWIMVDVTRTMELVDRLKADKEFKDVKVTPLLVLAKAMCLAARRHPEINA
                     AWDEAAQEIVVKHYVNLGIAAATPRGLIVPNIKDADLMTM"
BASE COUNT          582 a         1307 c         1245 g          539 t
ORIGIN      
        1 gggggactgg gcaggtggaa cggaagccac cgacattctg tgcgggtccg acatatgaga
       61 accgcccgca tgtggcctat cgtgagccgc tagcagagcg acagtggcaa cgacgccaag
      121 ggcgcccccg ccggcgatcc cggcacgcgc tcagcggccc gacgagaccc ggccaccctg
      181 ggtcagaagg agagctgtgt tcgatctacc gatccccgac ccatcgccgg ggcgccgtct
      241 cggcgacggc gggcccgaca tgatccaagt cctcacgccc gagggtgagc gagtcacctc
      301 ggcgccgcgt gagtacctcg agatcatcga cgcactgacc gacgaggacc tgcgcgggtt
      361 ctatcgcgac ctcgtcatga tccggcgctt cgacgccgag gccaccgccc tgcagcgcca
      421 gggtgagctt ggcctctggg ccagcctgct cgggcaggag gccgcccagg tcggctccgg
      481 tcgcgcgctg cgcccccagg actatgcctt cccgacctac cgcgagcacg gcgtcgcctg
      541 gtgccgcggg gtcgacccgg tcaacctcat ggggatgttc cgcggggtca accacggcgg
      601 gtgggacccc aacgagaaga acttccacct ctacacgatc atcatcggcg cccagaccct
      661 gcacgccacc ggctatgcca tgggtgtcca gcgcgatggc gcggtcggca ccggtgacga
      721 cgagcgggac acggccgtca tcgcctactt cggcgacggg gccaccagcc agggcgacgt
      781 cagcgagtca ctgatcttcg ccggcgtcaa caactccccg gtcgtcttct tctgccagaa
      841 caaccagtgg gcgatctccg agcccaacga gaagcagacc cgcgcgccgc tttaccaccg
      901 cgcgcgcggc ttcggcttcc ccggcctgcg ggtcgacggc aacgacgtcc tcgcggtgta
      961 tgccgtgacg aaggttgcgc tcgacgccgc ccgatcgggg caaggaccga gcttcatcga
     1021 ggcgttcacc tatcggatgg gcgcccacac gacctccgac gacccgacga agtaccgcat
     1081 gtccgccgag gtcgaggtgt ggaaactgcg cgaccccatc gagcggctca aggcctggct
     1141 cgcccacgaa ggcaaggccg acggtgcttt cttcgcccaa gttgcggcgg aggccgacga
     1201 gctcggcgcc gacgtccggg cccgctgcct cgccctgccc gacccggccg gcaccgacat
     1261 gttcgaccac gtctacggcg agcggcaccc ggtgatggag cgcgagcgag ccgagttcgc
     1321 ggcataccag gcgggttttg agggagcgaa ccatggctga gcgcacgcgc acggacggcc
     1381 ccgcggcata cccggtcacg accaccctcg cgaaggggat cacgagcggc ctgcgggcgg
     1441 cgatggagca cgaccccaag gtcgtcctca tgggcgagga catcggcaag ctcggcggcg
     1501 tgttccgcgt gaccgagggc ctgcagaagg acttcggcga ggaccgcgtc atcgacaccc
     1561 cgctggccga ggccggcatc atggggacgg cggtcgggct ggcactgcgc ggttacaagc
     1621 cggtggtgga gatccagttc gacgggttcg tctacaacgc gttcgaccac atggtgtcct
     1681 cgatcgccaa gatgcgggcg cgctcgctcg ggcatgtcgg gatgccgatc gtcgtgcgga
     1741 tccccttcgg cggcggcatc ggtgcggtcg agcaccacag cgagagcaac gaggcctact
     1801 tcgctcacac ggcggggctg cgggtcgtgg cctgctccaa cccgcacgac gcgcactgga
     1861 tgatccagca ggccattgcc tgccccgacc cggtcatctt ctacgagccc aagcggcgct
     1921 attgggacaa gggcgagatc gctgccgagc cgggtcgcgg cctcttccag gcggcgaccc
     1981 tgcgtcaggg ctccgacgtc acggtgctgg cctatgggcc gatggtgcgc acctgcctgc
     2041 aggccgctca ggccgctgcc gaggagggtc gcagccttga ggtcatcgat ctgcgctcgc
     2101 tctcgccgct ggacacgggg gccatcatcg agtcggtgcg ccgcaccggg cgttgtgtcg
     2161 tggtccatga ggcctcgacg ttcctcggcc tcggtgccga gattgccgcg ctcatccagc
     2221 aggagtgttt ctactcactg caggcgccgg ttgcccgcgt cgggggctac aacgtccctt
     2281 atccgcccag tcggatcgag gaggagttcc tccccgatct cgaccgcgtg ctcgatgccg
     2341 tcgaccgtct gatggcgtac tgaaagaggc tggctcgtga gtgtccgtga gttcctcctg
     2401 cctgacccgg gcgagggtct gaccgaagcc gagatcgtca cctggcgggt cgccgtcggc
     2461 gatgtggtga aggtcaacga catcgtcgtc gagatcgaga cggccaagtc gctcgtcgag
     2521 ttgcccattc cttgggcggg caccgtcgcc gcgctgctcg ccccggaggg ggcgaccgtc
     2581 gaggtcggca gcgcgatcat cgccatcgac gacccgttgt atgccggtcc gccaccgtcc
     2641 gcctccggcc ccgccgccgc ctcttccagt ttgaccgcca tggcgggcaa tctggtcggt
     2701 ggcgctgata gctcaacgcc ttccaccagt ttgggcgcca tggcggccaa acttgacggg
     2761 ggtggggctg gggctgggcc cgcaaccggc ggcacctccg gcggcacaac cggcggcacc
     2821 tccggaggcg ccgacgaggc ggtcgccgag gggctcatcg gtggcaccac gtcaaccggc
     2881 cgcacggcgg tgctcgtcgg ttacggcatc aagcagaccg aagccgtccg tcgccctcgc
     2941 cgcaaggacg agttggcccg caccgtcgct cgcccgccgg acaaggaggg cggccacatc
     3001 gagcagccgc cgtcggcgca catgggcggc ctggtcgccg cccacgcccc ggaagtgcgg
     3061 cgtgacaccc ggatgctcac cgagctcgcg ccggtcgtcg ctccgctcgc gcccgccccg
     3121 ggcgcgccgg tgctcgccaa gcccccggtt cgcaagtacg ccaaggatct cggcgtcgac
     3181 ctcgcccagg tccccacccc gaccgggatc gtcacccggg ccgacatcga cgcctacatc
     3241 cacggcggcg ctcccgacga gacggtgacg gccgcggcat acgcctcagg tatgccgtcg
     3301 cgcgcggacg cccgcggcga gacccgagtc ccgatcaagg gcgttcgcaa gatgaccgcc
     3361 gcggcaatgg tgggatcggc gttcacggct ccgcacgtca ccgagtggat catggtcgac
     3421 gtcacccgga ccatggagct cgtcgaccgg ctcaaggccg acaaggagtt caaggacgtc
     3481 aaggtgacgc ccctgctcgt gctggccaag gcgatgtgtc tggctgcgcg ccgtcatccc
     3541 gagatcaacg cggcctggga cgaggcagcg caggagatcg tcgtcaagca ctacgtcaac
     3601 ctcggcatcg ccgcggcaac cccgcggggg ctcatcgtcc ccaacatcaa ggacgccgac
     3661 ctcatgacga tgc
//
LOCUS       DAOMZY010000345         3666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_24194_length_3666_cov_5.370258, whole genome shotgun sequence.
ACCESSION   DAOMZY010000345 DAOMZY010000000
VERSION     DAOMZY010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24194_length_3666_cov_5.370258"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2257
                     /locus_tag="PLK64_15020"
     CDS             <1..2257
                     /locus_tag="PLK64_15020"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HPV81105.1"
                     /translation="DLQQTEERLRAFLHASPGDGRHTRTAVLALTCASVALTTTCLAG
                     HDSIRSAGSLLTWAGDRASVVVEAVADSDPRRVRAREDRSGEAEIVVLRARIVRVTAR
                     GATSQVDAPVLVIGGVDWVGLQWQEGFRARGRLAPAEAGDDVVAILRATGPPELTAEA
                     PLVARGAAYVRGRFREATARLPPDAAGLVPALVIGDTSATPVDLSEAMTVTGMSHLSA
                     VSGSNVAIVLAAAIGAARLAGVRRRWRPVLAVVVLAGFVVLARPEPSVVRAAVMGVVG
                     LVGLSASRRRAGVPALATSIVVLLCWDPWLARAYGFALSVLATLGLLILARPWGERLG
                     RLLPGRLARAGPTLAVPLAAQVMCAPVVVLLQASVSLVAVPANLVAAPFVAPATILGV
                     ATAVVSVIWVDAAAWLAWLAAIPTLAIAGVARVAASMPFGSLPWPAGPPGALLLGGLS
                     VLGLVTAPWLRFRLLQRPLAGLAIIAVTTAAAVPTRNVGWPMAGWSLVACDVGQGDGI
                     VLSTSAGHAVLVDAGLDPAVIDGCLRRLDVRVLDAVILTHFHADHVNGLPGALRGREV
                     REILTSAVQDPVFQVGAVGDWARAVGVAVKPLFAGDRLEWPGITARVWWPARVLHEGS
                     IPNNGSVVLTADVKGVHVVLLGDIEREAARAVGAAIRRDPQVPGWEVDVVKVAHHGSA
                     NIDPAVLDALPGAVAVISVGAENDYGHPAPSTLTDLRSRGFQILRTDLDGDIAVGRPS
                     EGTLMVATRGP"
     gene            complement(2258..2782)
                     /locus_tag="PLK64_15025"
     CDS             complement(2258..2782)
                     /locus_tag="PLK64_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HPV81106.1"
                     /translation="MDLRPGFPTVVDEVTVRLIASVVLVIGIVALVSGAWWLYAVLAA
                     DFILRSVFGPRLSPIAQVVLRTIRPRVPAAGRPTPGAPKRFAASIGAVLTSAATILAI
                     LHATSGSAAAGTAVFAIGAIMVVFPALESAAGLCVGCVIFAQLIRLGLVPERICLECA
                     DITRRTAVASGQPA"
     gene            complement(2859..>3666)
                     /locus_tag="PLK64_15030"
     CDS             complement(2859..>3666)
                     /locus_tag="PLK64_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HPV81107.1"
                     /translation="EQRTGTALVERLGRNVRLTEAGEVLARHAVTVLAALDAAEEEVA
                     AIAGLRSGRVRLMAFPSSSASLVPRALALLKKRYPEVSVRFSEGEPPESLAALRAGDC
                     DLAVAFAYEGTDVGRGEDLDPFIIRPLMDDEVRLAMPPDHPLAAQSVINLADVADDPW
                     IAGCPRCRGHLVTLAHGAGFSPNVAFETEDYVAVLGLVRAGLGVALVPDLILEAAHED
                     GIVARPLSPPSRRQIHAVTTEDLQRVPAVAATLEALEESARSRAKVPVRS"
BASE COUNT          485 a         1226 c         1383 g          572 t
ORIGIN      
        1 cgacttgcag cagactgagg aacgacttcg ggcgttcttg cacgcgagcc ctggggatgg
       61 gcggcatacg agaaccgcgg tgttggccct cacctgcgcg tccgtcgcgc tgacgacgac
      121 gtgtttggcc ggccacgact cgattcgcag tgccgggtcg ctgctgacct gggcgggcga
      181 ccgggccagc gtcgtcgtcg aggcggtggc ggacagcgac cctcgccggg tgcgggcgag
      241 ggaggatcgg tcgggcgagg ccgagatagt cgtcctgcgg gccaggatcg ttcgggtcac
      301 tgctcgcggc gccaccagcc aggtcgacgc ccccgtgctg gtcatcggtg gcgtcgattg
      361 ggtcgggctg cagtggcagg agggcttccg ggcccgaggg cggctggctc ccgccgaagc
      421 gggggacgat gtcgtcgcca tccttcgggc cacggggccg ccggagctca ccgccgaggc
      481 gccgctggtg gcgagggggg cggcatacgt ccggggtcgg ttccgcgagg ccacggcgcg
      541 gctccccccg gatgcggcgg gcctcgtccc cgcgttggtc atcggggaca cgtcggcgac
      601 acccgtcgac ctgtccgaag cgatgaccgt caccggcatg tcgcacctgt ccgcggtgag
      661 cggctcgaac gtggccattg tcctggccgc ggccatcgga gccgcgcgac ttgcgggagt
      721 ccggcgtcga tggcgaccgg tcctcgcagt ggtcgtcctg gccggcttcg tggtgctggc
      781 gcgcccggag ccgagcgtcg tgcgagcggc cgttatgggg gtggttgggc tcgtcggttt
      841 gagtgcctcc cgtcgccgcg ctggcgtgcc ggcgctcgct acctcgatcg tcgtcctgct
      901 gtgttgggac ccgtggctcg cccgggccta cggattcgcg ttgtcggtgc tggccacgct
      961 cgggctgctg atcctcgctc gtccctgggg cgaacgattg gggcggctgc tgcctgggcg
     1021 gttggcccgc gcgggaccga ccctggcggt gccactggct gctcaagtca tgtgtgcccc
     1081 ggtcgtcgtc ctcctgcagg cctcggtctc tctggtggcg gttccggcca acctggtcgc
     1141 cgcaccgttc gtcgcgccgg ccacgatcct cggcgtggcg acagccgtcg tttccgtgat
     1201 ctgggtggat gccgccgcgt ggctcgcgtg gctggctgcg atcccgactc tcgccatcgc
     1261 gggtgtggcc cgcgtggccg cgagtatgcc gttcggcagc cttccgtggc cggccgggcc
     1321 gcctggtgcc ctactgctcg gtggcttgtc ggtgctcggg ctcgttactg ctccgtggct
     1381 gcgcttccgc ctgctgcagc gccctcttgc gggtctggcg atcatcgcgg tgaccaccgc
     1441 cgccgccgtc ccgacgcgga acgtcgggtg gccgatggct ggctggtccc tggtggcctg
     1501 cgacgtcggg cagggcgacg gcatcgtctt gtccacgtct gccgggcatg ccgtgctcgt
     1561 cgacgccggg ctggacccgg cagtgatcga cggctgtctg cgccgactgg acgtgcgagt
     1621 cctcgacgcc gtgatcctca cccacttcca tgccgaccac gtcaacggcc tgcctggggc
     1681 gcttcggggc cgggaggtgc gcgagatcct cacctctgcg gtgcaagacc cggtcttcca
     1741 ggtgggagcc gtcggcgact gggcccgggc cgtcggggtt gcggtcaagc cgctcttcgc
     1801 cggagatcgg ctggagtggc cagggatcac ggcgcgggtg tggtggcccg cccgagtcct
     1861 gcacgaggga tcgatcccca acaacgggtc ggtcgtcctg accgccgacg tcaagggcgt
     1921 ccacgtggtg ctgctgggcg acatcgagcg ggaggctgcc cgcgctgtcg gtgcggcgat
     1981 ccgtcgagat ccgcaagtgc caggctggga ggtcgatgtt gtcaaggtgg cccatcatgg
     2041 gtcggccaac atcgacccag ccgtgcttga cgcgcttccc ggtgccgtcg cggtgatcag
     2101 tgtgggggcg gagaacgact acggccatcc cgcgccgtcc acgctcaccg acctgcgcag
     2161 tcgaggcttt cagatcctgc ggaccgacct cgacggggac atcgcggtcg gtcggccgag
     2221 cgaaggcaca ctcatggtgg ccactcgcgg cccgtgatca ggcaggctga ccggaggcca
     2281 cagccgtccg gcgggtgatg tcggcgcact ccaggcagat gcgctccggc accaggccga
     2341 gccggatcag ctgagcgaag atgacacacc cgacgcacag cccagccgct gactccaacg
     2401 cgggaaagac gaccatgatc gcgccgatgg cgaagacggc ggtccccgca gcggccgatc
     2461 cgctcgtcgc gtggaggatg gccaggatcg tcgcggccga cgtcaggaca gcaccgatgc
     2521 tcgccgcgaa ccgcttgggg gctccaggcg tcgggcggcc cgcagccggc acgcgaggcc
     2581 gaatcgtgcg cagcacgacc tgggcgatcg ggctcaacct cggcccgaag acactgcgca
     2641 ggatgaagtc ggcggcgagg acggcgtaca gccaccaggc ccccgagacg agggcgacga
     2701 tgccgatcac gagcacaacg ctcgcgatca ggcgaacggt gacctcgtcg acgacggtgg
     2761 ggaacccagg gcgaagatcc atgtcattga ttatggaacg aataagggtt ccttatgtga
     2821 cgtgcccgcg atgtggacag ccgggccgcc gctgcaccct aggaacgcac ggggaccttg
     2881 gcgcgggagc gggcgctctc ttccagtgcc tcgagcgtgg cggcgacggc cgggacccgc
     2941 tgcagatcct cggtcgtcac cgcgtggatc tggcggcggg acggcgggct gagtggccgg
     3001 gcgacgatgc cgtcctcgtg ggcggcttcg aggatgaggt cggggacgag cgcgaccccg
     3061 aggccggcgc gcaccaaccc gaggacggcg acgtagtctt cggtctcgaa ggcgacgttg
     3121 ggggagaacc ctgcgccgtg agcaagcgtg accaggtgac ctcggcagcg cgggcagccg
     3181 gcgatccacg ggtcgtcggc gacatcggcg aggttgatga ccgattgagc ggccaacggg
     3241 tggtccggcg gcatggcgag gcggacctcg tcgtccatga gtggccggat gatgaaggga
     3301 tcgaggtctt cgccgcggcc gacgtcggtg ccctcgtagg cgaacgcgac ggccagatcg
     3361 cagtcaccag cgcgcagcgc ggcgagcgat tcgggcggct cgccctcgga gaaacgcacg
     3421 gacacctcgg ggtagcgctt cttgagcagc gccaaggcgc gcggcacaag cgacgctgac
     3481 gacgagggga acgccatgag ccgcaccctc ccggagcgca ggccggcgat cgccgcgacc
     3541 tcctcctctg cggcatcgag ggccgccagg acggtgacgg cgtgccgggc caggacttcg
     3601 ccggcttcgg tgaggcgcac gttgcgtccg agccgctcca cgagcgccgt gcccgtgcgc
     3661 tgttcc
//
LOCUS       DAOMZY010000346         3643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_24411_length_3643_cov_6.858696, whole genome shotgun sequence.
ACCESSION   DAOMZY010000346 DAOMZY010000000
VERSION     DAOMZY010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24411_length_3643_cov_6.858696"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..200
                     /locus_tag="PLK64_15035"
     CDS             6..200
                     /locus_tag="PLK64_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015488919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="HPV81108.1"
                     /translation="MTTSTVATVTEAERERRVAEAIHSGEMEGLSVSPAGRADAQEYI
                     AGRIDSDELVARARARYGLD"
     gene            187..765
                     /locus_tag="PLK64_15040"
     CDS             187..765
                     /locus_tag="PLK64_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020015802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPV81109.1"
                     /translation="MASTEFVDPYLDPESGILRNKVGARTKVALEDAEGDLSFARLMQ
                     LMDHPPKATGDVDELRAIHRHLFQDVYVWAGEARIVDVRKNVEGADWFLPVLMIGRAA
                     GYAAEELRSDNHLRGLDRESFIERLAYHYDQFNYIHPFREGNGRTQRVFWNRVARDAS
                     WQLDWRGVHGSTNWILRSVRATVPLVGEPVFR"
     gene            complement(846..1589)
                     /locus_tag="PLK64_15045"
     CDS             complement(846..1589)
                     /locus_tag="PLK64_15045"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPV81110.1"
                     /translation="MIDIETLTRLKMMRLSGMAEYFENLADTPTAATMTGPELVKQAV
                     DWEYERRRDSKLHRLRRQAGLAQPGADLADLKAMPGRSVDSELIARLAVGSYLLKRQD
                     VILQGPTGAGKTYVACALANKACQQYRKVLYLSAGELFDRITIAERTGDKKRCLDTLV
                     KVELLIIDDWFLTPPTRPQVQQLHTLIDRRHKTASTIYCTQLPPGQWHDRMEEKILAD
                     AIVDRITTNAHATVLDCDDSMRKHFTHLE"
     gene            complement(1586..3121)
                     /gene="istA"
                     /locus_tag="PLK64_15050"
     CDS             complement(1586..3121)
                     /gene="istA"
                     /locus_tag="PLK64_15050"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HPV81111.1"
                     /translation="MVDYKQILRLRAEGVSQRGIADALGCSRNTVAAVFTAADSAGVG
                     FGQVADLGADEVRTLLLPEPAKPDSDRAAPDFEYVHRELGRPSVTLLVLWNEYVAKCR
                     ATGEVPYQYSFFNEQYRRWVKSTGASMRIQRSPGESVEVDWAGDPMVFADPLTGELRQ
                     AWVFVAALSYSAYSYVEALPDMTLGSWIDAHVHAFEHFGGVGRLLVPDNLRTGVSRSD
                     RYEPALNPAYARLAEHYGTAVIPARVKRPRDKAVVEGSVRFVAGQVAAVLRNRQFVGL
                     AELNEAIFDEVAAINARPFQKREDSRLMVFERDEKPLLIPLPPVRFELADLRKAKAGP
                     NYHVQVDRNFYSVPARLIGQSLDVRLTSATVEVFDGLERVASHPRFTGVFGRYSTLPA
                     HMPEQHRSRLVDWTPERFEQWASTIGPHTVAAIRAILASRKIVEQSYRSCLGVMSLAK
                     KPGGIARLEDTCSRALAATPAPSYTLIKKLWAGWTPAAPPPAPSLGDAGFVRGADYYG
                     SRA"
BASE COUNT          598 a         1189 c         1241 g          615 t
ORIGIN      
        1 ccggtatgac cacatcgacg gtcgcgacgg tcaccgaggc ggagcgcgag cggcgcgtcg
       61 ccgaggcgat ccacagcggt gagatggaag gcctgagcgt gtcgccggct ggccgagctg
      121 acgcccagga atacatcgcg gggcgcatcg actccgacga gctggtggct cgcgcacgcg
      181 cacgctatgg cctcgactga gttcgtcgac ccgtacctcg accccgaaag cgggatcctg
      241 cggaacaagg tcggcgcacg aacgaaggtc gccctcgagg acgccgaggg cgacctttca
      301 tttgcccgcc tgatgcagct catggatcat ccgccgaagg cgactggtga tgtcgacgag
      361 ctgcgcgcga tccatcgcca cctgttccag gacgtctacg tgtgggccgg cgaggcgcgc
      421 atcgtcgacg tgcgtaagaa cgtcgaggga gccgactggt tcttgccggt cttgatgatc
      481 ggccgtgcgg ccggctacgc cgccgaggag ttgcggtccg acaaccatct ccgcgggctc
      541 gaccgtgaga gcttcatcga gcgcctggcg taccactacg accagttcaa ctacatccac
      601 ccgttccggg agggcaacgg tcgtacgcag cgagtgttct ggaaccgtgt cgcccgtgac
      661 gcgagctggc agctcgactg gcgtggcgtt cacgggtcga cgaactggat attgcgctcg
      721 gtcagagcca ctgttccgct ggtgggtgag ccagtgttcc ggtgaccggt gggccacttc
      781 gacggggttg ggtgagccgt ggcgacgtca ggggcgggcc ttgatggccc gtccctgacg
      841 cttggtcact ccaggtgggt gaagtgcttg cgcatggagt cgtcacagtc gaggacggtg
      901 gcgtgcgcgt tggtggtgat ccggtcgacg atcgcgtcgg cgaggatctt ctcctccatc
      961 cggtcgtgcc actgcccggg tggcagttgg gtgcagtaga tcgtcgaggc ggtcttgtgg
     1021 cgccggtcga tgagggtatg gagctgttgg acttgtggcc gggtcggtgg cgtgaggaac
     1081 cagtcgtcga tgatcaacaa ctcgaccttg acgagggtgt cgaggcagcg tttcttgtcg
     1141 ccggtgcgtt cggcgatcgt gatccggtcg aacagttccc cggccgacag gtagaggacc
     1201 ttgcggtatt gctggcaggc cttgttcgcc agggcgcagg cgacgtaggt cttcccggct
     1261 ccggtgggtc cttgcaggat gacgtcttgg cgtttgagca ggtaggagcc gacagccagc
     1321 cgagcgatca gttcgctgtc gacgctgcgg ccgggcatgg ccttgaggtc ggccagatcg
     1381 gctccgggtt gcgccaggcc tgcttgccgg cggagccggt gcagcttgct gtcgcgccgt
     1441 ctctcgtatt cccagtcgac ggcctgtttg accagctcgg ggccggtcat ggtggccgcg
     1501 gtgggggtgt cggcgaggtt ctcgaagtac tcggccatcc cggacaggcg catcatcttg
     1561 agccgggtga gggtttcgat gtcgatcatg ctcggctccc gtagtagtcg gcgccgcgca
     1621 cgaacccggc gtcgcccagc gatggcgcgg ggggtggtgc ggcgggtgtc cagccggccc
     1681 acagcttctt gatcagcgtg tacgacgggg cgggggtggc tgccaacgct cgactgcagg
     1741 tgtcctcgag gcgggcgatg ccgccgggct tcttggccag cgacatcacc cccagacacg
     1801 accggtagga ctgctcgacg atcttgcggg acgcgaggat cgcccggatc gcggccaccg
     1861 tgtgggggcc gatggtcgag gcccactgct cgaaccgttc cggggtccag tccacgagcc
     1921 ggctgcgatg ctgctcgggc atgtgcgcgg gcagggtgga gtaccggccg aacacgccgg
     1981 tgaaccgtgg atgcgacgcg acgcgctcca agccgtcgaa cacctccacg gtggccgaag
     2041 tgagccggac atccaaggac tgcccgatca gccgggcggg gaccgagtag aagttccgat
     2101 cgacctggac gtggtagttc ggtcccgcct tggccttgcg gaggtccgcc agttcgaacc
     2161 ggaccggtgg tagcgggatc agcagtggtt tctcgtcgcg ttcgaacacc atcaggcggg
     2221 agtcctcccg cttctggaac ggtcgggcgt tgatggcggc gacctcgtcg aagatggcct
     2281 cgttcaactc ggccaacccg acgaactgcc ggttgcggag caccgcggcg acctgcccgg
     2341 cgacgaaccg gacactgcct tcgacgaccg ccttgtcgcg gggacgcttc acgcgggccg
     2401 ggatgaccgc cgtcccgtaa tgctccgcca accgcgcgta cgccgggttc aacgccggct
     2461 cgtaccggtc cgacctggac accccggtac gcaggttgtc cgggacgagg agccggccga
     2521 cacccccgaa atgctcgaac gcgtgcacgt gcgcgtcgat ccacgacccc aacgtcatgt
     2581 cgggcaaggc ctccacatag gagtaggccg agtacgacaa cgcggccacg aacacccacg
     2641 cctgcctcag ttcgccggtc agcgggtcgg cgaacaccat cggatcgccg gcccagtcga
     2701 cctcgaccga ctcgcccggg ctgcgttgga tccgcatcga cgcccccgtc gacttcaccc
     2761 accggcggta ctgttcgttg aagaacgagt actggtacgg cacctcgccg gtggcgcggc
     2821 acttggccac gtactcgttc cacaacacca gcagtgtcac cgacggccgt cccagctccc
     2881 ggtgcacata ctcgaaatct ggtgcggccc ggtccgagtc cggtttggcc ggctccggca
     2941 gcaacagggt gcggacctcg tcagccccga ggtccgccac ctgcccgaac ccgacaccgg
     3001 ccgagtccgc cgcagtgaac accgccgcca ccgtgttacg cgaacacccc aacgcgtcag
     3061 caatcccgcg ctggctcaca ccctcggcac ggagcctgag gatctgtttg tagtcgacca
     3121 tgacggacga ctcctctcgt caacgcccgt gccccagcgg cacgaacgcc gacgaacgtt
     3181 ccacacgcgc agaccagcac cggcgccacg gctcaccccg gaccgtcacg gtggcccact
     3241 ggtcaccgga acactggctc acccaccaac ggaacagtgg ctctcaccca gcgcaatatg
     3301 cacctcgtcg gctcgttgcg ttggccgata gcttcggagg ggagtggatg tgccggctgg
     3361 tcgcggaacg aagaaacccc cggtcgcatc atggttcaag accagagagg cagaatccgg
     3421 ggactcctgc gtttagcgta cgtcccatgc tcagcaacgg ccaacagcaa ctggcatggc
     3481 tggcatgacg ccaccctcca tcgcagcgct cggtgacttt cgcgcaggag gctgcgctcg
     3541 gcagatcgcg gcaaaggaac ccgccgagac ggtgtctgat tcccaggggg caacctcacc
     3601 gacaagcctg aacaggaggg cagttcagtc gtctgtcccg tcg
//
LOCUS       DAOMZY010000347         3633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_24515_length_3633_cov_3.375908, whole genome shotgun sequence.
ACCESSION   DAOMZY010000347 DAOMZY010000000
VERSION     DAOMZY010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24515_length_3633_cov_3.375908"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PLK64_15055"
     CDS             complement(<1..373)
                     /locus_tag="PLK64_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019311245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC family protein"
                     /protein_id="HPV81112.1"
                     /translation="MSHSTTPFTLLPSPSSDRAIESIRRSSPHSQPPEYNIRRNWPGL
                     SNNTIMIWAQRPDATLVAGYRAWQAKGRQVRRGETAIKVYGPVTMREPELDPGGKSVR
                     GPDGKPVMQVRIVGVKPVSVFD"
     gene            461..1546
                     /locus_tag="PLK64_15060"
     CDS             461..1546
                     /locus_tag="PLK64_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPV81113.1"
                     /translation="MSAVPGSAEALLSGGMSLLAPEETVFTAMLDGWQVQQRSRLLVA
                     TTIKQRAFAVRRFHRFAGDYPWQWTPADVEEWTSELVGGGVSHSTIRHYQMAVSLFCG
                     YVTDPRYRWTELCEQQFGSHPVQVFHEWNTVVHRAEYEGRPGNRPFTREELQVFFDYC
                     DQRVVDVAGGGRKGWLAAYRDATLFKVIYAWGLRRREAAMLETVDWSANAAADQFGRY
                     GALSVRYGKAVKGSPPRRRTVLTTMGWAAEAVAEWVTDIRPLYATSGIGLWPTERQGK
                     ISTDTLSQRFQTYREAAGLDPTLGPHCLRHSYATHLLEDGYDHLFVQQQLGHAWGSTT
                     AIYTSVGADYKNQALRRALSRALDQPR"
     gene            1623..1913
                     /locus_tag="PLK64_15065"
     CDS             1623..1913
                     /locus_tag="PLK64_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007273216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPV81114.1"
                     /translation="MADAGMFATTDLVPLLAERGVVLSPAQVYRLVTGTPERLSLRTL
                     VALCDILGCTPNQLIEPVVEARTARATGTTGATPATTTAKKRTPRRAEITRE"
     gene            1994..3313
                     /locus_tag="PLK64_15070"
     CDS             1994..3313
                     /locus_tag="PLK64_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81115.1"
                     /translation="MRDTCTDCARPNQPLDHRLRCQWCRRNDHKHCHDCRRPGAGLVG
                     YDGVQVCQHCALRRELDKVIPAHPDGTLAALRPVVLAAEPLTTRRWLRRAHTLLDDLN
                     QERTRLDHASLDQLPQRRAVEHLRALLIATNILPPDPAGPVRRLEASLGQALAHLDPA
                     HRRTIRRWARWKLLPPLRQRVDQNRDLGTSVANARRRIEQTAAFVATLEHRGRTLTET
                     TQHDIDQWFSGPGASRWNARPFLAWCQQHHHLPTRLELPPQYRGKATAPTDAEQRWTI
                     ADQLANDDHLDPADRVAGLLVVLYAQPLSRIVTLTRNDVLTDNDGRLQLRLGPDPLDL
                     PEPLATLIQTLPVTRRRSTAQQLPNPWLFAGGHAGAHTNATNLGARLRRIGIEPRRMR
                     LAAAEQLSREIPPAMLAGVLGLRTANLSRHTTESGGNWANYAATPRT"
BASE COUNT          593 a         1425 c         1079 g          536 t
ORIGIN      
        1 catcgaacac gctcaccggc ttcaccccga ctatccgcac ctgcatgacc ggcttcccgt
       61 cggggccgcg caccgacttg ccacccgggt cgagctcggg ttctctcatg gtgacgggtc
      121 cgtaaacctt gatggcggtt tcgccgcggc ggacttggcg gcctttggcc tgccaggctc
      181 ggtagccggc gaccagtgtc gcgtcgggcc tctgcgccca aatcatgatc gtgttgttgg
      241 aaaggcccgg ccaattgcgt ctgatgttgt attccggcgg ttgactatgc gggctggagc
      301 gtcggatgga ctctatcgcg cgatcggagc tgggacttgg caacagcgta aatggcgttg
      361 tggaatggct catgctggtt acctggttgc atctgatgcg tgatacgttc atgtgcttgt
      421 gagggctgtt gagggggttg ccgctggttc ggcggggggc gtgtccgctg tgcccgggtc
      481 ggctgaggcg ctgctgagcg gcgggatgtc gctgctggct cccgaggaga cggtgttcac
      541 cgcgatgctg gacggctggc aggtgcagca gaggtccagg ctgctggtcg cgaccacgat
      601 caagcagcgg gccttcgcgg tccgccggtt ccaccggttc gccggcgact atccgtggca
      661 gtggaccccg gccgatgttg aggagtggac ctccgaactg gtcggcggcg gggtgtcgca
      721 ttcgaccatc cgccactacc agatggccgt gtcgttgttc tgtggctatg tcaccgaccc
      781 gcgttaccgc tggacggagc tgtgcgagca gcagttcggc tcgcatccgg ttcaggtctt
      841 ccacgagtgg aacacggtgg tgcaccgggc cgagtacgag ggccgtccgg ggaaccggcc
      901 cttcacccgc gaggagcttc aggtgttctt cgactactgc gaccagcggg tcgtcgacgt
      961 ggccggcggc ggtcggaagg gctggctggc ggcctatcgg gacgcgaccc tgttcaaggt
     1021 gatctacgcc tggggcctgc gccgccggga agcggccatg ctcgagaccg tcgactggtc
     1081 ggccaacgcc gcggctgacc agttcggccg ctacggcgcc ttgtcggtgc gctatggcaa
     1141 ggcggtgaag ggatccccac cgcgccgtcg gaccgtgctg accacgatgg gttgggccgc
     1201 cgaggcggtc gccgagtggg tcaccgacat ccggcccctc tacgccacgt ccgggatcgg
     1261 gttgtggccg accgaacggc agggaaagat ctccaccgac accctgagcc aacgcttcca
     1321 gacctaccgg gaagcggccg gcctggaccc caccctcggg ccgcactgcc tgcggcactc
     1381 ctacgccacc cacctgcttg aggacggcta cgaccacctg ttcgtgcaac agcaactcgg
     1441 ccacgcctgg ggctcgacta ctgccatcta caccagcgtc ggagccgact acaagaacca
     1501 ggcgctgcgc cgcgccctgt ctcgcgccct cgaccagccc cgctgatccc gaccattgga
     1561 ggaacctgat gccccgagcc cggaaacctg ccctcggata ccgctggcac ctgcgcggcc
     1621 tgatggccga cgccggcatg ttcgccacca ccgacctggt cccgctgctg gccgaacgtg
     1681 gcgtcgtgct ctctccagcc caggtatatc ggctggttac cggcacccct gaacggctca
     1741 gcctgcgcac cctggtcgcc ctctgcgaca tcctgggctg caccccgaac cagctcatcg
     1801 aacccgtcgt cgaggcgcgg accgcacgcg ccaccggcac caccggcgcg accccagcca
     1861 ccaccaccgc gaagaaacgc accccgcgac gagccgagat cacccgcgag tgaccccgct
     1921 ctgccgaatc tgcgcccgtg agacccggcc cgacaggaaa acgtgctggc gctgcctcaa
     1981 agggatcggc agggtgcgcg acacctgcac cgactgcgcg cggcccaacc aacccctcga
     2041 ccaccgcctc cgctgccagt ggtgccgccg caacgaccac aaacactgcc atgactgccg
     2101 ccgacccggc gccggcctgg tcggctacga cggagtccag gtctgccagc actgcgccct
     2161 gcgccgcgaa ctcgacaagg tcatccccgc ccaccccgac ggcaccctgg ccgcgctgcg
     2221 ccccgtcgtc ctcgccgccg agcccctcac cacccggcgc tggctccgcc gcgcccacac
     2281 cctcctcgac gacctgaacc aggaacgcac ccgtctcgac cacgccagcc tcgaccagct
     2341 tccccaacgc cgcgccgtcg aacacctccg agcgctgctg atcgccacca acatcctgcc
     2401 gcccgaccct gccgggcccg tccgtcggct cgaagccagc ctcggccagg ccctcgccca
     2461 cctcgacccc gcacatcgcc gcaccatcag acgatgggcc cgctggaagc tcctgccacc
     2521 gctgcggcaa cgagtcgacc aaaaccgtga cctcggcacc tccgttgcca acgcccgccg
     2581 ccggatcgag cagaccgcag ccttcgtggc caccctggaa caccgcggac gcaccctgac
     2641 cgagacgacc caacacgaca tcgaccaatg gttctccgga ccaggcgcgt cccgctggaa
     2701 cgcccgcccc ttcctcgcct ggtgccaaca acaccaccac ctgcccaccc ggctcgaact
     2761 gccaccccag taccggggca aagccaccgc ccccaccgac gccgaacaac gctggaccat
     2821 cgccgaccaa ctcgccaacg acgaccacct cgaccccgcc gaccgggtcg ccggcctgct
     2881 cgtcgtcctc tacgcccaac cgctcagccg catcgtgacc ctcacccgca acgacgtcct
     2941 caccgacaac gacggacgcc tgcaactgcg cctcggaccc gacccgctcg acctccccga
     3001 accactcgcc accctgatcc agaccctgcc cgtcacccga cgccgcagca ccgcccaaca
     3061 gctccccaac ccctggctct tcgccggcgg gcacgctgga gcccacacca acgccaccaa
     3121 cctcggcgcc cgcctgcgac gcatcggcat cgaaccccgc cgcatgcggc tcgccgccgc
     3181 cgaacaactc agccgcgaaa tcccacccgc catgctcgcc ggcgtcctcg gcctgcgcac
     3241 cgccaacctc tcccgacaca ccaccgaatc cggcggaaac tgggccaact acgcagccac
     3301 cccccgcacc tgacagccac cactcacggc gttcgagtat gccggtactg ccagtcctgt
     3361 actggtggaa gctgttggcg aacgtcagcc acgacgtcca cgcgtccatc gaggcaagct
     3421 tgccgacctg ctcggccagc tgggtgtgca tcgtctccag agtcgcctgc cgggcggcct
     3481 ccgcttcctc acggtcgtac ccggaccgtc gaccggtggt gttcgccact ggactcaccg
     3541 gccctgcagc agggcgactt gggcgagcag gtcgtcgatc accgccgggt cgccgccaag
     3601 atcgcggaga tcctcgacct gacacagcag gtc
//
LOCUS       DAOMZY010000348         3626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_24585_length_3626_cov_7.003640, whole genome shotgun sequence.
ACCESSION   DAOMZY010000348 DAOMZY010000000
VERSION     DAOMZY010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24585_length_3626_cov_7.003640"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1507
                     /locus_tag="PLK64_15075"
     CDS             <1..1507
                     /locus_tag="PLK64_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015936312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HPV81116.1"
                     /translation="VILGLVFLKYVSDAYDERRAAVEADLRAQGMDDDQVAMLIDDPE
                     EYQGSGVFVVPARARWEFLAERAKGQQASMNEHSKTVGQLIDEAMDAVMIANPALSGT
                     LPRIFNRDNVDQKRLGELLDLFNNARFSRMGEGRARDLLGEVYEYFLEKFARAEGKRG
                     GEFYTPASVVKVLVEVLQPDHGRVYDPCCGSGGMFVQAEKFIYDHHGDPQNIKVYGQE
                     LNERTWRMAKMNLAIHGIENSGIHTRWGDTFVLDYHADKQMDFVMANPPFNIKDWNRI
                     EKDPRWRYGVPPAGNANYAWIQHILSKLTDDGRAGVVMANGSMSSNSGGEGEIRARIV
                     EADLVSCMVALPTQLFRSTGIPVCLWFFAKDKSAGKQGATDRTGQVLFIDARELGFMV
                     DRAERSLAAEDIARIAETFHAWRGSSSAVEQGLSYADVPGFCASVSLADIKAAEYALT
                     PGRYVGAAEIEDDGEDIDAKIERLTADLFAAFDESSRLERAVRQQMERLHV"
     gene            1500..2588
                     /locus_tag="PLK64_15080"
     CDS             1500..2588
                     /locus_tag="PLK64_15080"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HPV81117.1"
                     /translation="MSDWYSRPLGDLITLQRGFDITRDQQARDGAVPVVSSGGISSYH
                     DTAMANGPGVVIGRKGTLGRVHFVDVPYWPHDTTLWVKDFKGNDPRFVYYALMMLDTE
                     FLNVGSASPTLNRNHLHPLPVSWPSLPEQRAIAEVLGALDDKIAANTTLVQVADALAG
                     SLTRRALSSARVPLREVAALTMGSSPPGTSYNEEGNGAVFYQGVRDFGVRFPQNRVWT
                     DAPVRMAAKRDTLVSVRAPVGRTNLAAEETCIGRGLAGVHSRSGHAMSLFHLLRDDPT
                     VWEPFEAEGTVFGSINKHQLEGLLVPVVAAEAADSLESQLLAVEGRIAAALAENVALA
                     KARDELLPLLMSGKVRVKDAEKVVEGVV"
     gene            2588..>3626
                     /locus_tag="PLK64_15085"
     CDS             2588..>3626
                     /locus_tag="PLK64_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HPV81118.1"
                     /translation="MSGLNGMSEDDWEHLALDHLAESDVWEPRHGTTIAPGSGLRTSW
                     ADPVMPSVLMAALVRLNPEVPTQHLQQALADILAPKSQDAITENCRIHGYLVDGYRGL
                     SYTDTDGREENPTIRLLSERADANVYHAVNQVIVRAGDHQRRFDIVLYVNGMPLAVLE
                     LKRAGSAHADVAAAHAQLETYVRELPLAFRFCVVTVASDGILAKYGTPFTPLNHYAPW
                     NVDDDGKLLTLDDTLDGEHRTGLEVLLDGLFNAERFLQIIRDYTAFDSTTKGLVKRIA
                     KPHQYFAVSNAVASTIAAVKSNGKAGVVWHTQGSGKSMEMELYTARVIRHPALLNPTI
                     VVITDRTELDGQ"
BASE COUNT          630 a         1198 c         1208 g          590 t
ORIGIN      
        1 cgtcatcctc gggctggtgt tcctcaagta cgtctccgac gcctacgacg agcgtcgcgc
       61 cgccgtcgag gccgaccttc gtgcgcaagg gatggatgac gaccaggtcg cgatgctcat
      121 cgacgacccg gaggagtacc agggctctgg ggtcttcgtc gtccccgccc gggcccggtg
      181 ggagttcctc gccgagcggg ccaaggggca gcaggcgtcg atgaacgagc actccaagac
      241 cgtcggccag ctcatcgacg aagccatgga tgccgtgatg atcgccaacc cggccctctc
      301 cggcaccctg ccccgcatct tcaaccgcga caacgtcgac cagaagcgcc tcggtgagct
      361 gctcgacctg ttcaacaacg cccgcttcag ccggatgggg gaggggcgcg cccgcgacct
      421 gctcggtgag gtctacgagt acttcctcga gaagttcgcc cgggccgagg gcaagcgggg
      481 cggggagttc tacacgccgg ccagtgtcgt caaggtgctc gtcgaggtgc tccagcccga
      541 ccacggccgc gtgtacgacc cgtgctgcgg ctcggggggc atgttcgtgc aggcggagaa
      601 gttcatctac gaccaccacg gcgacccgca gaacatcaag gtctacgggc aggagctcaa
      661 cgagcgcacc tggcggatgg cgaagatgaa cctcgcgatc cacggcatcg agaactcggg
      721 catccacacc cggtggggtg acaccttcgt gctcgactac cacgcggaca agcagatgga
      781 cttcgtgatg gcgaaccccc cgttcaacat caaggactgg aaccgcatcg agaaggaccc
      841 gcgctggcgc tacggcgtgc cgcccgcggg gaacgccaac tacgcgtgga tccagcacat
      901 cctgtccaag ctcaccgacg acggccgggc cggcgtcgtc atggccaacg ggtcgatgtc
      961 gagcaacagt ggcggggagg gcgagatccg ggcccggatc gtcgaggccg acctcgtgtc
     1021 gtgcatggtc gccctgccga cgcagctctt ccgaagcacc ggaatccctg tgtgcctgtg
     1081 gttcttcgcg aaggacaagt cggccggcaa gcagggggcg accgaccgga ccggtcaggt
     1141 gctcttcatc gacgcccgcg agctcggctt catggtcgac cgagcggagc ggtcgctggc
     1201 ggccgaggac atcgcgcgga tcgcggagac cttccacgcg tggcgcggtt ccagttccgc
     1261 tgtggagcag ggtctctcgt atgccgacgt gccgggattc tgcgcgtcgg tgagcttggc
     1321 ggacatcaag gctgccgagt acgccctgac gcccggccgg tacgtcggcg cagcagagat
     1381 cgaggacgac ggcgaggaca tcgacgccaa gatcgagcgc ttgaccgccg atctcttcgc
     1441 ggccttcgac gagtcgagcc gcctcgaacg tgcggtgcgt caacagatgg agcggctcca
     1501 tgtctgactg gtacagccgc ccgttgggcg acttgattac gctccagcgc gggtttgaca
     1561 tcactcgaga ccagcaggcc cgagatggcg cggtcccagt tgtttcgtcg ggcggaatct
     1621 cctcgtatca cgacacggca atggccaatg gccccggggt tgtcattggg cggaagggca
     1681 ctttgggtcg tgtccacttc gtggatgtgc cgtattggcc gcacgacacc actctgtggg
     1741 tgaaggactt caagggcaat gacccgaggt tcgtctacta cgccctgatg atgcttgaca
     1801 ctgagtttct gaacgtcgga tctgcaagcc cgaccctcaa ccgcaaccac cttcatcccc
     1861 taccggtcag ttggccgagc ctgcctgagc aaagggcgat tgcggaggtg ttgggggcgc
     1921 tcgacgacaa gatcgccgcc aacaccactt tggttcaggt cgccgatgct ttggctggca
     1981 gtctgacgcg gcgtgcgcta tcgagtgcgc gagtgcctct gcgagaggtc gcggcgctca
     2041 ccatggggtc gtcaccgccc gggaccagct acaacgagga gggcaacggc gctgtcttct
     2101 accagggcgt ccgagacttc ggggttcgct tcccgcagaa cagggtgtgg accgatgcgc
     2161 cggtcaggat ggccgcgaag cgtgacacgc tggtcagcgt cagggcgcca gtcggccgga
     2221 cgaacctggc ggcggaggag acctgcatcg gccggggctt ggctggcgtc cactcccgct
     2281 ccggtcacgc gatgtccctg ttccacctgc ttcgcgacga tcccacggtg tgggagccct
     2341 tcgaggctga ggggacagtc ttcggctcga tcaacaagca ccaattggag ggcctgcttg
     2401 tcccggtcgt tgccgcggag gctgccgatt cattggagag ccagttgcta gccgttgagg
     2461 gacggatcgc cgcagccttg gccgagaacg ttgcgctcgc caaggctcgt gacgagttgt
     2521 tgccgctgct gatgtcgggc aaggttcggg tcaaggacgc cgagaaggtc gtcgaggggg
     2581 tcgtgtgatg tccggtctga acggcatgag cgaggacgac tgggagcacc tggccctcga
     2641 ccacctcgcc gagtccgatg tgtgggagcc gcgccacggc acgacgatcg cccccggctc
     2701 gggcctgcgg acgtcgtggg ccgacccggt gatgccgtcg gtgctcatgg cggcactcgt
     2761 tcggctcaac cccgaggtgc cgacgcagca cctccagcag gcgctcgccg acatcctcgc
     2821 cccgaaatcg caggacgcga tcaccgagaa ctgccgcatc cacggctacc tcgtcgacgg
     2881 gtaccgcggt ctgtcctaca ccgacaccga cggccgggag gagaacccga cgattcggct
     2941 gctcagcgag cgggctgatg ccaacgtcta ccacgcggtc aaccaggtca tcgtccgcgc
     3001 cggcgaccac cagcgccggt tcgacatcgt gctctacgtc aacggcatgc cgctggccgt
     3061 cctcgagctc aagcgtgccg gctccgccca cgccgacgtc gccgctgcgc acgcccagct
     3121 cgagacctac gttcgtgaac tgccgttggc gttccggttc tgcgtggtca cggtggcgag
     3181 tgacggcatc ctcgcgaagt acggaacccc gttcaccccg ctcaaccact acgccccgtg
     3241 gaacgtcgac gacgacggaa agctgctcac cctcgacgac accctcgacg gcgagcaccg
     3301 caccggactc gaggtgctgc tggacgggct gttcaacgcc gagcggttcc tgcagatcat
     3361 tcgcgactac acggccttcg acagcacgac caagggcctg gtgaagcgga tcgccaagcc
     3421 gcaccagtac ttcgcggtga gcaacgccgt cgcgagcacc atcgccgcgg tcaagagcaa
     3481 cggcaaggcc ggcgttgtgt ggcacacgca gggctcgggc aagtcgatgg agatggagct
     3541 gtacaccgcg cgggtgatcc ggcacccggc gctgctcaac ccgacgatcg tcgtcatcac
     3601 cgaccgcacc gagctcgacg ggcagc
//
LOCUS       DAOMZY010000349         3625 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_24599_length_3625_cov_4.413165, whole genome shotgun sequence.
ACCESSION   DAOMZY010000349 DAOMZY010000000
VERSION     DAOMZY010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24599_length_3625_cov_4.413165"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..781
                     /locus_tag="PLK64_15090"
                     /pseudo
     CDS             1..781
                     /locus_tag="PLK64_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772436.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
     gene            785..>1779
                     /locus_tag="PLK64_15095"
     CDS             785..>1779
                     /locus_tag="PLK64_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPV81119.1"
                     /translation="MSETTTAAPVAAAVDFAALFPEEVVTKALVRDVTMPGQAGTMAL
                     VTLDNGFDHTKPNTFGPASIAGLQACFDGLRARAEAGEIQAVGLTGKPFIFAVGADLS
                     GVPKVTQREQALAIARAGHAAYATLMDLPVPTFAFVNGAAMGGGVEIALACDYRTISS
                     GVPAVALPECFLGLVPGWGGCYLLPNLVGPAKALTMIVENALNTNKMLKGPAAFELGV
                     ADAMFAPAEFLEASIGWAAKVVSGAVTVTRPEVPRDEAAWTGAIAASTMLADLKTGKQ
                     SPAPYRAMKLVAAARTATRDEAFAAEDEALADLLMGDELRAGLYAFDLVQKRAKRP"
     assembly_gap    1780..1879
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1880..2860
                     /locus_tag="PLK64_15100"
     CDS             <1880..2860
                     /locus_tag="PLK64_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018778672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HPV81120.1"
                     /translation="AQLDGLAKKGRLNPDKLNRFKALVSGSTSKAGFADAEFVIEAVF
                     EEMGVKKQVFAECEAVVSDTCVLATNTSSLSISQMAADLAHPERVVGFHFFNPVAVMP
                     LLEIIPGSTTDPATLATAFAVGAKLKKTCILVKDSPSFIVNRLLGRFMGEVGRVADAG
                     TPVEVIDQAFMGFAPMPPFVLLGLVGPAIALHNGETLIKAFPDRFYVSEGLKAIVEAK
                     KPGFYTFAGGKPALDPEVLALLPQPENPVVLTAPEVRERVLGALAQEARMMLDEGVAQ
                     APMDIDLAMITGAGFQFWNGGLTPLLDRSGVAEQVAGARFLPAGAASVPA"
     gene            2968..>3625
                     /locus_tag="PLK64_15105"
     CDS             2968..>3625
                     /locus_tag="PLK64_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV81121.1"
                     /translation="MSEDYRSRIGTLIRDARRHKGLTQTELGRLLGTSQSAVVRIEQG
                     HQNLSLEMLARIGEALDRELVTLGNSRPQHLRIVGGAKLSGAIDVKTSKNAAVALLCA
                     TLLNRGRTTLRNLARIEEVNRIIEVLSSMGVKVRWLPDSNDLEIVPPSALDLEGIDEE
                     AARRTRTVIMFLGPLLHDRAEFRLPYAGGCDLGTRTVEPHLAALRSFGLDVVATHGFY
                     E"
BASE COUNT          545 a         1258 c         1161 g          561 t
ORIGIN      
        1 cccgtccgcg ctggtcatgg gccagaccgc cgagaacctc cacgaccgct tccctcagct
       61 gaccaaggcc cgctgcgatg ccttcgccct cggctcccag ggcaagctcg ccgccgccta
      121 tgccgcaggc aagatccagc ccgacctcgt ctccgtcgcc actcggcaca aggagttggg
      181 ctacggcctc gcgaccgtcg acgagccccc gaggcccagc accaccatgg ccgacctcga
      241 agcgctcaag acgcccttcc gggtgcgcgg caacgtcacc gccggcaaca gcgcgggcct
      301 caacgatggc gccaccgcgt gcctgctcgc ctccgaggac gccgccgtgg ccatgggtat
      361 gccggtccgc atgcggttgg catcgttcgc cttcgtcggc gtcgagcccg aggtcatggg
      421 catcgggccg gtgcctgcca ccgagaaggc cctggccaag gcgggcctga cgatcgagga
      481 catcgggctg ttcgagctca acgaggcctt cgccgtgtag gtcgtcgcct tcctcgacca
      541 cttcggcatc gccgacgacg accctcgggt caacgaatac ggcggcgcga tcgccatcgg
      601 tcaccccctc gcgtcttcgg gcgtccgcct gatgacccaa ctggcccgcc agttcgaaga
      661 gcaccccgag gtgcgttacg gcctgactgc gatgtgtatc ggcatcggca tgggcggcgc
      721 cgtcatctgg gagaaccctc accacgccga ctacgtcgcg tcggccagca aggaggcctg
      781 agccatgagc gagaccacta ctgccgcccc tgtcgccgct gccgtcgact tcgccgcgct
      841 cttcccggag gaggtcgtca ccaaggccct cgttcgagac gtgaccatgc cgggtcaggc
      901 aggcacgatg gcgctggtca ccctcgacaa cggcttcgac cacaccaagc ccaacacctt
      961 cggcccggcg agcatcgccg ggttgcaggc ctgcttcgac ggcttgcgtg ctcgcgccga
     1021 ggccggagag atccaggcgg tcggcctcac cggaaagccg ttcatcttcg cggtcggcgc
     1081 cgacctctcc ggagtcccca aggtgaccca gcgcgagcag gcgctggcga tcgctcgagc
     1141 cgggcacgcg gcatacgcca ccctcatgga cctcccggtc ccgaccttcg cgttcgtcaa
     1201 cggcgcggcc atgggcggtg gggtcgagat cgccctggcg tgcgactacc gcacgatctc
     1261 ctcgggcgtc cccgcggtgg cgctgcccga gtgcttcctc gggttggtcc ccggctgggg
     1321 tggctgctac ctgttgccca acctggtcgg cccggccaag gccttgacga tgatcgtcga
     1381 gaacgcgctc aacaccaaca agatgctcaa gggcccggcg gcgttcgagc tgggtgtggc
     1441 cgatgcgatg ttcgcgccgg cggagttcct cgaggcatcc atcggttggg cagccaaggt
     1501 cgtttcgggc gccgtgaccg tgacccggcc ggaggtcccc cgcgacgagg ccgcctggac
     1561 gggcgccatc gcggcgtcga cgatgctggc cgacctcaag acgggcaagc agtcaccggc
     1621 tccgtaccgg gcgatgaagc ttgtcgcagc cgcgcgcacc gccacccgcg acgaggcctt
     1681 cgccgccgag gacgaggcgc tggccgacct tctcatgggc gacgagctcc gcgccggcct
     1741 ctatgccttc gacctggtgc agaagcgcgc caagcgcccn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnng cccagctcga cgggctggcc aagaagggcc gactgaaccc
     1921 cgacaagctc aaccggttca aggcgctggt cagcgggtcg acgagcaagg ccggcttcgc
     1981 cgatgccgag ttcgtcatcg aggccgtctt cgaggagatg ggcgtcaaga agcaggtctt
     2041 cgccgagtgc gaggccgtcg tctccgacac ctgcgtcctg gcgaccaaca cctccagcct
     2101 ctcgatctcg caaatggcgg ccgacctcgc ccaccccgag cgagtggtgg gcttccactt
     2161 cttcaacccc gtcgccgtta tgccgttgtt ggagatcatc cccggctcga ccaccgatcc
     2221 ggcgaccttg gccacggcgt tcgccgtcgg agccaagctg aagaagacgt gcatcctcgt
     2281 caaggactcg ccgtccttca tcgtcaaccg gctgctcgga cggttcatgg gtgaggtggg
     2341 tcgggtcgcc gacgccggca cgcccgtcga ggtcattgac caggccttca tgggcttcgc
     2401 gccgatgccg cccttcgtcc tgctcggact ggtcggtccg gcgatcgcgc tgcacaacgg
     2461 cgagaccctc atcaaggcct tccccgaccg gttctacgtc tccgagggtc tcaaggcgat
     2521 cgttgaggcc aagaagcctg gcttctacac ctttgccggc ggcaagccgg ccctcgaccc
     2581 cgaggtgctc gctctgctcc cccagcctga gaacccggtc gtcctcacgg cgcctgaggt
     2641 gcgcgaacgg gtgctcggcg ccttggcgca ggaggcccgg atgatgctcg atgagggggt
     2701 ggcccaggcg ccgatggaca tcgacctggc catgatcacc ggtgccggct tccagttctg
     2761 gaacggcggg ctcaccccgc tgctcgaccg cagcggagtc gccgagcagg tcgccggagc
     2821 ccggttcctc cccgccggag ccgcgagcgt tcctgcctag ggacaaatcc gtcaggaggt
     2881 atgccgcccg cagaagcggg cggcatacct gcgcctgagg cgccccacgc ggctcgcgac
     2941 atctcatctg cgagatacga tggcggcatg tcggaggact atcgcagccg gattggcacg
     3001 ctcatccggg atgccagacg gcacaagggg ctcactcaga ccgagttggg ccgcttgctg
     3061 gggacgagtc agagcgcggt ggtgcggatc gagcagggcc accagaactt gtcgctggag
     3121 atgctcgccc gcattggaga agccttggac cgcgagttgg tgaccctcgg gaattctcgc
     3181 ccgcagcatc tgcggatcgt cggtggggcc aagttgtcgg gcgccatcga cgtcaagacc
     3241 tccaagaacg ccgcggtcgc cctgctgtgc gcgacgctgc tcaaccgcgg ccgcacgacg
     3301 ttgcgcaacc tggcccggat cgaggaggtc aaccgcatca tcgaggtgtt gtcctcgatg
     3361 ggggtcaagg tgcggtggct gccggactcc aacgacctgg agatcgtgcc gcccagcgca
     3421 ctggacctgg aggggatcga cgaggaggct gctcgacgca cccgcacggt catcatgttc
     3481 ctcgggccgc tgctgcacga ccgcgccgag ttccgactgc cctatgccgg aggctgcgac
     3541 ctcggcaccc gcaccgttga gccgcacctg gcggcgctgc gatccttcgg gttggatgtc
     3601 gtggcgaccc acgggttcta cgagg
//
LOCUS       DAOMZY010000350         3622 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_24627_length_3622_cov_6.281189, whole genome shotgun sequence.
ACCESSION   DAOMZY010000350 DAOMZY010000000
VERSION     DAOMZY010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24627_length_3622_cov_6.281189"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="PLK64_15110"
     CDS             complement(<1..1112)
                     /locus_tag="PLK64_15110"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPV81122.1"
                     /translation="MTSPRVRIAAAGALVLAAALSIPAASASADPRHPHGQSARVPIQ
                     LLSFNDYHGHLEATDPALPSKWDPSQSPTGGVEYLSATVKALRAEVGDSNSLTVAAGD
                     LIGGSPFLSGMFHDEPSVESLETLGLDVSSVGNHEFDEGSAELLRMQRGGCHPVDGCY
                     YPDQPYDGASFTWLAANVVKKSNGKPLLPPVAVRAVKGVKIGFIGMTLEATPTLVNPA
                     GVASVDFKDEIETANAQAAALKKRGVKTIVVLLHEGGVNPSGISSCAGISGPILAIAQ
                     GITHEVDAIITGHTHEPYICQIPDADGVPRLVTSAASYGQVVTETTLVVDKATGEVDR
                     SASVAENHLVLRSIGKDPAETAVIAKWNTLAAPMAAR"
     gene            complement(1258..1767)
                     /locus_tag="PLK64_15115"
     CDS             complement(1258..1767)
                     /locus_tag="PLK64_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81123.1"
                     /translation="MSSQPGRVPFGALTLDYPLSIGVFGTYSEAQRAVDALSDAGFPV
                     QNCLIVGTDLRQLERVTGRLTWGRVLLGGALSGLWLGLFVGLIFSLFVTTQGGVPPVF
                     STMVFGAVFGAVWAAIGYAATRGRRDFTSVSRIVPSRFEVLAEHKVAQQARELLAKAG
                     IVTAGPPTV"
     gene            complement(1850..3052)
                     /locus_tag="PLK64_15120"
     CDS             complement(1850..3052)
                     /locus_tag="PLK64_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPV81124.1"
                     /translation="MSRLQTTDGLTDDQKELLKLVKEFVDEQIIPVATELEHRDEYPQ
                     AIVDGMKEMGIFGLMIPEEYGGLGESLLTYALCVEEIARGWMSVSGIINTHFIVAYML
                     LQHGTEEQKQRYLPRMATGEVRGAFSMSEPALGSDVSAIKTKAVKDEQGDGWTINGQK
                     MWLTNGGSSNLVAVLVRTETGADSVYKNMTTFLVEKESGFGETAPGLTVPGKIEKMGY
                     KGVDTTELVFEDYRTNDAQILGGVPGKGFYQMMDGVEVGRVNVAARACGLSMRAFELG
                     IRYSQQRETFGKKIAEHQGILFRLADMAVKVEASHQMMVKAARLKDAGKRNDLEAGMA
                     KYLAAENCADVVEQSFRIHGGYGYSKEYEIERLYREAPMLLIGEGTAEIQKMIIGRRL
                     LEDYALKR"
     gene            complement(3198..3572)
                     /locus_tag="PLK64_15125"
     CDS             complement(3198..3572)
                     /locus_tag="PLK64_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HPV81125.1"
                     /translation="MSHGKDVLDALRDETAALREKIPGVYEGFAQTHKAALGDGALST
                     KAKELIAFGIAVSKQCDGCIASHARGAARAGATLEEAAEAIGVAILMNGGPATVYGPR
                     AYAAFSEFHAQFARLRGAQTPG"
BASE COUNT          587 a         1223 c         1186 g          626 t
ORIGIN      
        1 cgggctgcca tcggcgcggc cagggtgttc cacttggcga tgaccgcggt ttccgccggg
       61 tccttgccga tcgagcgcag cacgaggtgg ttctccgcca ccgacgcgga ccggtcgacc
      121 tcaccggtgg ccttgtcaac gacgagggtc gtctcggtga cgacctggcc gtaggaggcc
      181 gcgctggtga ccagccgcgg gacaccgtcg gcgtcaggga tctggcagat gtagggctcg
      241 tgggtgtgcc cggtgatgat cgcgtcgacc tcgtgggtga tgccctgggc gatagccagg
      301 atcgggcccg agatgccggc gcacgagctg atgccactcg ggttgacgcc gccttcgtgc
      361 aggaggacga cgatggtttt gacgccgcgc ttcttcagcg cggccgcctg ggcgttggcc
      421 gtctcgatct cgtccttgaa gtcaaccgac gccacgcctg ccgggttgac caatgtcggc
      481 gtggcctcga gggtcatccc gatgaatccg atcttgacac ccttgaccgc gcgcaccgcc
      541 acagggggca gcaacggctt gccattggac ttcttcacaa cgttggccgc gagccacgtg
      601 aacgacgccc cgtcataggg ctggtccggg tagtagcacc cgtccaccgg gtggcagccg
      661 ccgcgctgca tgcgcagcaa ttcagccgag ccttcgtcga actcgtggtt gccgacgctg
      721 ctgacgtcca gcccaagggt ctccagggat tccaccgacg gctcgtcgtg gaacattccg
      781 gagaggaacg gcgacccgcc gatgaggtcg cccgcagcga cggtgaggct gttggagtcg
      841 ccgacctcgg cccgaagagc cttgacggtt gccgagagat attccacacc gccggtaggc
      901 gattggctag ggtcccactt gctcggcaga gcgggatcgg tggcttccag gtgcccgtgg
      961 tagtcgttga acgacagcaa ctggatcggc acccgggccg actgcccgtg tgggtgcctc
     1021 ggatcggccg aggcggacgc ggcggggatc gacagcgcag cggcgaggac cagagccccg
     1081 gccgcagcga tgcgcacgcg tggtgacgtc atggaagtcc ttcccttcga ggggtccgcc
     1141 gttcggcggc ccactaccga ggcaggctag cccggtaccg cacggaacgc gcggtgtacc
     1201 ggaatgaacg ttgcccggcc agtggtgggc aacgcgtcga caggtatgcc ggtcggctca
     1261 gaccgtgggc ggccccgccg tgacgatccc cgccttcgcg agcaactccc gggcctgctg
     1321 cgcgaccttg tgctcggcca gcacctcgaa cctgctcgga acgatgcggc tcacggaggt
     1381 gaagtcgcgt cggccgcggg tcgcggcata cccgatggcg gcccagaccg ccccgaaaac
     1441 cgcgccgaac accatcgtcg agaagaccgg cgggaccccg ccctgcgtgg tgacgaacaa
     1501 cgagaagatc aacccgacga atagcccaag ccacaagcct gagagtgccc cgccgagcag
     1561 gacccgcccc caggtgagtc gaccggtcac ccgctcgagt tgacgcaggt cggtgccgac
     1621 gatgagacag ttctgcacag ggaacccggc atccgacagg gcatcgactg ctcgctgggc
     1681 ctcggagtag gtcccgaaga ccccgatcga cagcgggtag tcgagggtga gggcaccgaa
     1741 gggaacgcga ccgggttgag aactcatagc gcaagtctca cacggctggc cgtcggtcgg
     1801 tccccgtgtg acgccggccg atcaccactc cccgactcgt gccggcccat caccgcttga
     1861 gggcgtagtc ctccaacagg cgtcggccga tgatcatctt ctggatctcc gccgtgccct
     1921 caccgatgag cagcatgggc gcctcgcggt agaggcgctc aatctcgtac tccttggaat
     1981 agccatagcc gccgtggatc cggaaggact gctcgacgac gtccgcacag ttctcggcgg
     2041 cgaggtattt cgccatgccg gcctcgaggt cattgcgctt acccgcgtcc ttgagccgcg
     2101 ccgccttgac catcatctgg tggctcgcct cgaccttgac cgccatatcg gccagccgga
     2161 agaggatgcc ttggtgttcg gcgatcttct tgccgaaggt ctcgcgctgc tgggagtagc
     2221 ggatgccgag ctcgaaggct cgcatcgaca ggccgcaggc gcgagcggcg acgttgaccc
     2281 ggcccacctc gacgccgtcc atcatctggt agaagccctt gccggggact ccgccgagga
     2341 tctgcgcgtc gttcgtgcgg tagtcctcga acaccaattc ggtggtgtcg actcccttgt
     2401 agcccatctt ctcgatcttg cccggaacgg tgagccccgg tgcggtctcg ccgaacccgc
     2461 tctccttctc gacgaggaag gttgtcatgt tcttatagac cgagtcggcc ccggtctcgg
     2521 tgcgcacgag gaccgcgacg aggttgctgg acccaccgtt ggtgagccac atcttctgcc
     2581 cgttgatggt ccagccatcg ccctgttcgt ccttgacggc cttggtcttg atcgccgaca
     2641 catccgaccc gagggccggc tcgctcatcg agaaggcacc gcgcacctcg ccggtcgcca
     2701 ttcgcgggag gtaccgctgc ttctgctcct cggtaccgtg ctgcagcaac atgtaggcaa
     2761 cgatgaaatg cgtgttgata atgccgctga ccgacatcca gccgcgcgcg atctcctcga
     2821 cgcacagggc gtaggtgagc agcgattcac cgaggccgcc gtactcctcg ggaatcatca
     2881 gcccgaagat tcccatctcc ttcataccgt cgacgatggc ctgtggatat tcgtcgcggt
     2941 gctccagctc ggtcgccacg gggatgatct gctcatcgac gaactccttg acgagcttga
     3001 gcagctcctt ctggtcgtcg gtgagaccgt cggtcgtctg gagtcgtgac atggctacct
     3061 cgcgggttca ggcgtgcgga caggagactg ctgaacagca caggacacta ctgccgagta
     3121 tgccgcccgt caccgttggc aggatgacgc cgccatgtga gtcccggcac cctgcgtcgc
     3181 ccccagactc ccctcgatca gcccggcgtc tgcgcgccgc gcaaccgagc gaactgggcg
     3241 tggaactcgc tgaacgcggc atacgcgcgc ggaccataga ccgtcgccgg cccaccgttc
     3301 atgaggatgg ccacgccgat cgcctcggcc gcctcctcca acgtggctcc ggcgcgagcc
     3361 gcgccgcgcg catgcgaggc gatgcacccg tcgcactgct tgctcaccgc gatcccgaag
     3421 gcgatgagct ccttggcctt ggtcgagagc gcgccatcgc cgagggcggc cttgtgggtc
     3481 tgcgcgaagc cctcgtagac gccggggatc ttctcgcgca gggctgctgt ctcgtcgcgc
     3541 agcgcgtcga gaacgtcctt gccgtggctc atgcgggctc cttcgaccgt gggcggcata
     3601 cccgaatgac cctagtcgga tc
//
LOCUS       DAOMZY010000351         3612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_24721_length_3612_cov_5.623278, whole genome shotgun sequence.
ACCESSION   DAOMZY010000351 DAOMZY010000000
VERSION     DAOMZY010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24721_length_3612_cov_5.623278"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="PLK64_15130"
     CDS             complement(<1..562)
                     /locus_tag="PLK64_15130"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HPV81126.1"
                     /translation="MAESAAYAAAPVAAAAVSDPVIAKVTDIVADLTGYPADLLDPDL
                     DLEADLGVDTVKQAEVFAAVREHYGVERDDAMKLRDFPTLRHVAGWVRGKAGLPEPTA
                     APVAAPAAPVVAAAAPVASPVVSAPVAPAYAEPAPVAAPAGDSVIDKVIEIVAQLTGY
                     PADLLDPDLDLEADLGVDTVKQAEVFA"
     gene            complement(511..>3612)
                     /locus_tag="PLK64_15135"
     CDS             complement(511..>3612)
                     /locus_tag="PLK64_15135"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HPV81127.1"
                     /translation="FVEVGPKKALHGFVEDVLGSHHDDVLALFTNFPKLADDAAFNQA
                     LCGLYAAGLGLRPAAPVAAPVAAAPVVAASAPAPVAASAAAAPAAAGAAGAPSDSVIT
                     ELGKLFAGVLEQGMKLYGFPGGAAGAPGAGDTAYPAAAPAAPEGAPTAPAQEPVVITG
                     AGLGLPGVDPMFDDANVARILGGQQFISQLPATVRTKMARMRVTRLVKDAATGSGSFQ
                     VIDNEADVVKLAGQHAPLDVVAQYGIDPGRDAALDTTTRMALGAGFDALRDAGIPLVM
                     HYKKTTIGSQLPDRWGLPHSMRDETGIIFASAFPGYHNLIDEVSHYSEDRARREHLLA
                     LEGVRTHLNGSEPVCAEIDALIAQLKRELDENPFDFDRRFLFRVLSMGHSQFAEIIGA
                     RGPNTQVNAACASTTQAVSIAEDWIRSGRARRVIVISADDATGEQLMPWVGTGFLASG
                     AAATDERVEDAATPFDRRRHGMIIGAGAAALVVESAEAARERGIQPIAQVLGSVIANS
                     AFHGTRLDIDHISGVMETVVTEAERWGIDRHVIAPSLMFMSHETYTPARGGSAAAEIN
                     ALRKAFGADADKIVITNTKGFTGHAMGAGVEDVVAVKALETGLVPPVPNYKEPDPDLG
                     ALNLSKGGSYPVGYALRLAAGFGSQVAMSLLRYVPMPDGRRRAPNELGYAYRIVDQAA
                     WQRWLDSLSGHTGSQLEVDHRRLRIVDVGAPPVAVVSTTSVPVPYAAMNRSTGSAPAA
                     AVAVAPVVVAPAAAAAAPAPVVAPAPAPAPVAAPVAAPAPVPATPAAPAVVTDPVLDT
                     VTGIVAGLTGYPSDLLDPDLDLEADLGVDTVKQAEVFAAVREHYNVERDDTMKLRDFP
                     TLRHVAGWVRGKAGLPEPTAAAAPAPAAVAAPAAVAAPAAAAVVTDPVLDKVTEIVAG
                     LTGYPSDLLDPDLDLEADLGVDTVKQAEVFAAVREHYNVERDDTMKLRDFPTLRHVAG
                     WVRGKAGLPELTAAAAPAVSSGSPALPRTHPSSPPLQHRLRPLPRWPSPRHTLPPRWP
                     LPR"
BASE COUNT          537 a         1168 c         1414 g          493 t
ORIGIN      
        1 cggcgaagac ctcggcctgc ttgacggtgt cgacgccgag gtcggcctca aggtcaaggt
       61 cggggtccag caggtcggcc gggtagccgg tcagctgcgc cacgatctcg atgaccttgt
      121 cgatgactga atcgcccgca ggcgccgcga ccggtgccgg ctcggcatag gccggtgcca
      181 cgggggccga gacgacaggg gaggcgaccg gagccgcagc agcgaccaca ggggcggccg
      241 gtgccgcgac cggggcggcg gtgggctcgg ggagcccagc cttgccacga acccacccgg
      301 cgacgtggcg cagggtcggg aagtcgcgca gcttcatcgc gtcgtcgcgc tccacgccgt
      361 agtgctcacg caccgcggcg aagacctcgg cctgcttgac agtgtcgacg cccaggtcgg
      421 cctcaaggtc gaggtcgggg tccaagaggt cggccgggta gccggtcagg tcggcgacga
      481 tgtcggtcac cttcgcgatg accgggtcgc tcaccgcggc agcggccacc ggggcggcag
      541 cgtatgccgc ggactcggcc atcggggcag cggtcggagc cggtgctgca gcggcggtga
      601 gctcgggtgg gttcgtggca aggccggact ccccgagctc accgccggtg ctgcagcggc
      661 ggtgagctcg gggagcccgg ccttgccacg aacccacccg gcgacgtggc gcagggtcgg
      721 gaagtcgcgc agcttcatcg tgtcgtcgcg ctcgacgttg taatgctcac gaacggcggc
      781 aaagacctcg gcctgcttga cggtgtcgac gccgaggtcg gcttcgaggt caaggtcggg
      841 gtcgagcagg tcggacgggt agcccgtcag gccggcgacg atctcggtca ccttgtccag
      901 gaccgggtcg gtgacgaccg ctgcagccgc cggggcggcg accgccgcgg gtgcggcaac
      961 agcggccgga gccggtgcag cagcggcggt gggctcgggg agcccggcct tgccacgaac
     1021 ccacccggcg acgtggcgca gggtcgggaa gtcgcgcagc ttcatcgtgt cgtcgcgttc
     1081 gacgttgtag tgctcgcgga cggcggcgaa gacctcggcc tgcttgacgg tgtcgactcc
     1141 gaggtcggct tcgaggtcga ggtcggggtc gagcaggtcg gacgggtagc ccgtcaggcc
     1201 ggcgacgatc cccgtcacgg tgtccaggac cgggtcggtg acgaccgccg gagcggccgg
     1261 agtagccggg accggcgccg gagcggcgac gggtgcagcc acgggggccg gagccggggc
     1321 gggtgccacg actggcgcgg gagcagccgc agccgccgca ggggcgacga cgacgggcgc
     1381 gaccgcaacc gcagcagcag gtgcgctgcc cgtggaacgg ttcatcgcgg catacgggac
     1441 cggcaccgag gtggtggaga cgacggcgac cgggggggca ccgacatcga cgatccgcag
     1501 acgacggtgg tcgacctcga gttggctgcc ggtgtggccg gacagcgagt ccagccagcg
     1561 ctgccacgcg gcctggtcga cgatccggta ggcgtaaccc aactcgttgg gcgcgcgccg
     1621 acgaccgtcg ggcatgggga cgtaacgcag cagcgacatg gcaacctgcg agccgaagcc
     1681 cgccgccagg cgcagggcgt agcccaccgg gtaggaaccg cccttggaca ggttgagtgc
     1741 gccgaggtcg gggtcgggct ccttgtagtt ggggacgggc gggacgagcc cagtctccaa
     1801 cgccttgacg gcgacgacat cctcgacacc ggcgcccatg gcgtgaccgg tgaagccctt
     1861 ggtgttggtg atgacgatct tgtcggcatc ggcaccgaag gccttgcgca gggcgttgat
     1921 ctcagctgcg gcgctgccgc cacgggccgg agtgtaggtc tcgtgggaca tgaacatcaa
     1981 cgacggcgcg atcacgtgcc ggtcgatgcc ccaacgctcg gcctcggtga caacagtctc
     2041 catgacgcca ctgatgtggt cgatgtccaa ccgggtgccg tggaaggcgc tgttggcgat
     2101 gactgagcca agcacctggg cgatcggctg gatgccacgc tcgcgagcgg cctcggccga
     2161 ctcgacgacc agggccgccg cacccgcgcc gatgatcatg ccgtgccgac gccggtcgaa
     2221 cggcgtggcc gcatcctcga cccgctcgtc ggtggcggcg gcgccggagg cgaggaagcc
     2281 ggtgcccacc caaggcatga gctgctcgcc agtggcgtcg tcagcggaga tgacgatgac
     2341 ccgacgggcc cgcccggagc ggatccagtc ctcggcgatc gagacggcct gggtggtgct
     2401 ggcgcaggca gcgttgacct gcgtgttggg gccgcgggcg ccgatgatct cggcgaactg
     2461 cgagtgcccc atcgacagca cccggaagag gaaccggcgg tcgaagtcga acgggttctc
     2521 gtcgagctca cgcttgagct gggcgatcag ggcgtcgatc tcggcgcaga ccggctcgct
     2581 gccattgagg tgggtccgga cgccttcgag ggcgagcagg tgctcgcggc gggcgcggtc
     2641 ctcgctgtag tggctgacct cgtcgatgag gttgtggtag ccggggaacg ccgaggcgaa
     2701 gatgatcccg gtctcgtccc gcatcgagtg cggcaggccc caacggtcgg gcaactgcga
     2761 gccgatggtc gtcttcttgt agtgcatgac gagcgggatg cccgcgtcac gcagcgcgtc
     2821 gaagccggcc ccgagggcca tccgcgtggt cgtgtcgagg gcggcgtcgc ggcccgggtc
     2881 gatgccgtac tgcgctacga cgtccagcgg ggcgtgctgt ccggcgagtt tgacgacgtc
     2941 ggcctcgttg tcgatgacct ggaagctgcc cgaccccgtt gcggcgtcct tgaccagccg
     3001 ggtgacccgc atccgggcca tcttggtgcg gactgtcgcc gggagctggc tgatgaactg
     3061 ctggccgccg aggatccggg cgacgttggc gtcgtcgaac atcgggtcga caccgggcag
     3121 cccgagcccg gcgccggtga tgacgaccgg ctcctgcgcg ggggcggtcg gcgcaccttc
     3181 tggggcggcc ggggccgccg cagggtatgc cgtgtcgcca gcaccggggg cgcctgcggc
     3241 accgcccggg aagccgtaga gcttcatgcc ctgttcgagg acaccggcga agagcttgcc
     3301 caactcggtg atgaccgagt ccgagggcgc accggcagca ccggcagcgg ctggcgcggc
     3361 ggcggcagag gcggcaaccg gggccggagc cgatgcagcg accaccggag ccgcagccac
     3421 gggggcagcg accggagcgg ccgggcgcag acccagcccg gcggcataca gaccgcagag
     3481 ggcctggttg aacgcggcgt cgtcggcgag cttcgggaag ttggtgaaga gggcgagcac
     3541 gtcgtcgtgg tggctgccga ggacgtcctc gacgaagccg tgcagggcct tcttggggcc
     3601 gacctcgacg aa
//
LOCUS       DAOMZY010000352         3598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_24876_length_3598_cov_6.901496, whole genome shotgun sequence.
ACCESSION   DAOMZY010000352 DAOMZY010000000
VERSION     DAOMZY010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24876_length_3598_cov_6.901496"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..2089
                     /locus_tag="PLK64_15140"
     CDS             218..2089
                     /locus_tag="PLK64_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HPV81128.1"
                     /translation="MSVEVELAEVRDFLAAHHPFDDLPRQQLERVATTLKIEYFRRGT
                     RIISRGEDNHHLYVVRSGAVELHDMHGGFMDRGETGTSFGSYTLVQGNPANFEVVALE
                     DTLCLVMDAGTFHDLAAAYPIIGNYFDIQRTSRMRGAVASLHVTSSGGAILKTKVTDI
                     ITRPAVSVEASESIRAAASVMSQRRVSCLLVMDEGKLVGIVTDRDLRNRVVAAGVDTS
                     LPVSQVMSADPATGSADALAFQILMEMTSRNIHHLPILGCDGTPEGVVTTTDLMRLEQ
                     TNPIYLVGDITQATDVAAVAEVSKRLAPVVELLVTQDASAEDIGRIVTAVGDAVERRL
                     LGLAEEHLGPPPVPYCWVSLGSRARQEQALAADQDNALILDNDVQPEHAAYFEALATF
                     VCDALVECGYPRCPGDVMATNPRWRVPLSQWRAEFTTWLTEPVPDAILRASIFFDMRP
                     VYGDPRLFQKLRKHFLKQAPAARLFLAHLTKRATENEPPLGFFRGFVLEKHGANRATL
                     DIKRGGVGAIVEIARVLALSVGSRAVNTQTRIAAAVDSGALSAERGQDLHDAFEFISY
                     VRLRHQAAQVRADRETDNFVAPDDLSSFEKRHLREAFAIVRSAQSSLSHRYPSTYIS"
     gene            2098..2781
                     /locus_tag="PLK64_15145"
     CDS             2098..2781
                     /locus_tag="PLK64_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HPV81129.1"
                     /translation="MFATPSADKQRRRALRSAPSGPLRDYLQTPFPDPRTEVGDLQLL
                     AVDLETTGLHPKNDRLLSIGFVPIDGLSIDLSGARRFVVQAGVEVGQSAAIHGLTDDT
                     VAAGTPIDEALPVVLEALRGRVLLAHYTDIEERFLSAACERVYGTRLPVARIDTLELH
                     RRLIVGWGQEPMRLALRLWAARERYGLPDYRAHECLIDAIACAELYLAQVAEMRSHGD
                     SVTLARLVS"
     gene            complement(2841..3152)
                     /locus_tag="PLK64_15150"
     CDS             complement(2841..3152)
                     /locus_tag="PLK64_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81130.1"
                     /translation="MPRPNRRRDESTPLDLERATGGVPRREGYAGQEWFVRPVRGARA
                     DSAGAPRAYRCPGCQQEVGGQAHVVVWPADGLGGVEQRRHWHTTCWANRHTRPPLGSY
                     R"
     gene            complement(3336..>3598)
                     /locus_tag="PLK64_15155"
     CDS             complement(3336..>3598)
                     /locus_tag="PLK64_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPV81131.1"
                     /translation="KNRRSRHQPGQWLKITIERLNREIKRRARVVGIFPNSPAVIRLV
                     GAVLADAHDEWQVADRRYLSETSMALLYKTSDTDTAAITSGL"
BASE COUNT          518 a         1304 c         1206 g          570 t
ORIGIN      
        1 ccctcgccgg cggcaccctg gctggggtgc tgggagccct gttggcgatc ccgaccgccg
       61 ccggcctgtt gctgctctac gaagaggtcc tcgtccctcg ccagcagcgc cactgatcgc
      121 gaccgctcgt cggccccggt ggcacgctcg ccgcagcgtc gcgccagagc gctagggacg
      181 cctgacgcgg cgcctgggcg actgacagga tgacgccatg tccgtggagg tcgagctcgc
      241 cgaggtccgc gacttcctcg cggcacatca ccccttcgat gacctgccgc gccagcaact
      301 cgaacgcgtc gccacgacgc tgaagatcga gtacttccga cgcgggacgc ggatcatctc
      361 ccgcggcgag gacaaccatc acctctatgt cgtgcgctcg ggggccgtgg agttgcatga
      421 catgcatggc gggttcatgg accgcggcga gacgggcacg tccttcggct cctacacgct
      481 cgtgcagggg aatccggcca acttcgaggt ggtcgccctc gaggacaccc tgtgcctggt
      541 gatggatgcc gggaccttcc acgacctcgc cgccgcctac ccgatcatcg gcaactactt
      601 tgacatccag cggaccagcc ggatgcgcgg cgccgtcgcc tcgttgcacg tgacgagctc
      661 cggtggcgcg atcctcaaga cgaaggtcac cgacatcatc acccggccgg cggtgtccgt
      721 cgaggcgtcg gagtcgatcc gcgcggcggc atccgtcatg agtcagcgtc gggtctcgtg
      781 cctgctcgtc atggacgagg gcaaactcgt cggcatcgtc acggatcgcg acctgcgcaa
      841 ccgcgtcgtc gcggccggcg tcgacaccag cctgccggtg agccaggtga tgtcggcgga
      901 ccccgccacc ggatcggccg acgcgttggc gttccagatc ctcatggaga tgaccagccg
      961 caacatccac catctgccaa tcctcggctg cgacggcacg cccgagggcg tcgtcacgac
     1021 caccgacctc atgcgcttgg aacagaccaa ccccatctac ctcgtgggtg acatcaccca
     1081 ggcgaccgat gtcgcggcgg tggccgaggt cagcaaacgg ctggcgccgg tggtcgagtt
     1141 gctcgtcacc caggacgcgt cggccgagga catcgggcgg atcgtcaccg ccgtcggcga
     1201 cgcggtcgaa cgccggctgc tcggcctggc cgaggaacac ctcggcccac cgccggtccc
     1261 ctactgctgg gtctcgctgg gctcgcgggc ccgccaggag caggccctgg cggccgacca
     1321 ggacaacgcc ctcatcctgg acaacgacgt ccaacccgag cacgcggcat acttcgaggc
     1381 gctggcgacc ttcgtgtgcg atgccctcgt cgagtgcggc tacccccggt gcccgggaga
     1441 cgtcatggcg accaacccgc gctggcgggt cccgctctcg cagtggcgag ccgagttcac
     1501 cacctggctg accgagccgg tgcccgacgc catcctccga gcgagcatct tcttcgacat
     1561 gcggcccgtc tatggcgacc cgaggctgtt ccagaagttg cgcaagcact tcctcaagca
     1621 ggctccggcg gcccgactct tcctggccca cctcaccaag cgggccaccg agaacgagcc
     1681 cccgttgggc ttcttccgcg gcttcgtgtt ggagaagcac ggcgccaacc gggccactct
     1741 cgacatcaag cggggcggtg tgggcgcgat cgtcgagatc gcccgcgtgc tggccctgtc
     1801 ggtggggtcg cgggcggtca acacccagac ccggatcgcg gccgccgtcg actccggtgc
     1861 gctgagtgcc gagcgcggcc aggacctgca cgacgcgttc gagttcatct cctatgtgcg
     1921 gctgcgccac caagcggcgc aggtgcgggc cgaccgcgag accgacaact tcgtggcccc
     1981 cgacgacctg agctcgttcg agaagcgcca cctgcgtgag gcgttcgcca tcgtgcgctc
     2041 ggcccagtcg tcgctgtcgc accgctaccc ctcgacgtac atctcctagg ggcccgcgtg
     2101 ttcgccactc cttcggccga caagcaacgc cgtcgcgcgc tgcgcagcgc gccgtcgggg
     2161 ccgttgcggg actacctgca gacccccttc cccgaccccc gcaccgaggt cggtgacctg
     2221 cagttgttgg ccgtcgacct cgagacgacc ggcctgcacc ccaagaacga ccgcctgctc
     2281 tcgatcggct tcgtgcccat cgacgggttg tcgatcgacc tgtccggagc ccggcggttc
     2341 gtcgtgcagg ccggggtcga ggtcggtcag agcgcggcga tccacgggct gaccgacgac
     2401 accgtggccg ccgggacccc gatcgacgag gcgctgccgg tcgtgctgga ggccctgcgc
     2461 ggccgggtcc tgctcgcgca ctacaccgac atcgaggaac gcttcctgtc ggctgcctgc
     2521 gagcgggtct acggcactcg gttgccggtc gcccggatcg acacgctcga gttgcatcgc
     2581 cggttgatcg tcgggtgggg gcaggagccg atgcgcctag ccctgcgcct gtgggccgcc
     2641 cgcgagcgct acggcctgcc cgactatcgc gcccacgagt gcctcatcga tgccatcgcg
     2701 tgcgcggagc tctacctcgc ccaggtggcc gagatgcgct cccacggcga ctcggtgacc
     2761 ctcgcgcggc tggtgtcctg agcgatcgcg gtccgggtgc ctgacgccct gacggcccgc
     2821 gagggcgagg gcgggtcacc tcagcgatag gaccccaacg gcggcctggt atgccggttg
     2881 gcccagcatg tcgtgtgcca gtgccggcgc tgctcgacgc cacccaaccc gtcggccggc
     2941 cagacgacga catgggcctg gccgcccacc tcttgctggc agcccgggca acgataggcc
     3001 cgcggcgcgc ctgcggagtc cgcccgcgct ccccggacgg ggcggacgaa ccactcctga
     3061 ccggcatacc cctcgcgtct ggggactccc ccggtcgccc gctcaaggtc caacggtgtg
     3121 gactcgtcgc ggcggcggtt ggggcgaggc atgcacgcca tcctcccagg tcgccagggc
     3181 ggcgaggccg ccggacggtc cgcgagaacg cctcgcccac cggggccagt gtttccggag
     3241 cgctggccac accagagtgt gcccaacact ccgggaaccc gaccccgccg atagagcccc
     3301 ggcgggtggt gggctttgag gttggatcct cggtgctaca ggccgctagt gattgcggcg
     3361 gtgtcagtat cgctggtctt gtacagcagt gccatggagg tctcggatag gtagcggcgg
     3421 tcagcgactt gccattcgtc gtgggcgtcg gctaagaccg caccgacgag gcggatcacc
     3481 gccgggctgt tggggaagat cccgacgacg cgggctcgtc tcttgatctc gcggttcagc
     3541 cgttcgatcg ttatcttgag ccactggccg ggctggtgcc ttgacctgcg gtttttcg
//
LOCUS       DAOMZY010000353         3595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_24910_length_3595_cov_5.493503, whole genome shotgun sequence.
ACCESSION   DAOMZY010000353 DAOMZY010000000
VERSION     DAOMZY010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24910_length_3595_cov_5.493503"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="PLK64_15160"
     CDS             complement(<1..967)
                     /locus_tag="PLK64_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HPV81132.1"
                     /translation="MCLTGVDYFSTLGYQPGIAALAAGALAPLATLVLVLVTLLGALP
                     MYRRVAQESPHGDGSLSMLERLLTYWPSKLLVLVLIGFVATGFVITITLSAADASAHL
                     IENPFLKSVLSGQQVTVTLVLLAFLGAIFLKGFKEAIGIAVVLVAAYLGLSAVVVGRG
                     LMEVAAHSEHVGEWTSAITSTYASPMAVIGAALLVFPALALGLSGFETGVVVMPLVKG
                     DPTDTPAAPTGRIRNARKLLTTAALIMSVMLLGSSLVTTLLIPAADFQPGGEANGRAL
                     AYLDHDLLGDVFGTAYDISTIGILWFAGASAMAGLLNIVPRYLPRY"
     gene            complement(1209..2636)
                     /locus_tag="PLK64_15165"
     CDS             complement(1209..2636)
                     /locus_tag="PLK64_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HPV81133.1"
                     /translation="MGAFSWKLKGDGHTLAPGDVVAPDERLAWGKTIGLGAQHVVAMF
                     GATFVFPLLMGLNAQLAIMMSGIATIIFLLMVSGKVPSYLGTSASFVGVAAAIRAQPG
                     ADSRTVTGAILIGGVVLALVGLLVHVAGSGIIHKVLPPVVTGAVVMLIGFNLAPVVAN
                     VYWPQDQWVALLVMTFAIVCAVALKGFLGRISIFLSLVFGFVLSWLFDRGFGQITSFD
                     AGAGKVTTHWRVNLDGVASSPWLGFPPATTTGADGKELVGLHAPTISFAAVLVVLPAI
                     IALIAENTGHVKAVAEMTGADLDHVMGRAIAADGVATAVASSVGGSPTTTYAENIGVM
                     AATRIYSTAAYVVAAFIAIIFGLSPKFGALVAATPGGVLGGITVVLYGMIGLLGAKIW
                     KENNVDFANPINLVPVAAGIILGIGNTALKVSDTFELAGIALGTIVTIAAYHLARAFA
                     HADLRAAHDGGAMLTVGHEGIDKVK"
     gene            complement(2794..3552)
                     /locus_tag="PLK64_15170"
     CDS             complement(2794..3552)
                     /locus_tag="PLK64_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPV81134.1"
                     /translation="MSEPSGMPPRLTLQPLDESFDSALVIVAHPDDIEYGTAAAVDKW
                     TKAGKTVTYLLATRGEAGIDSIAPEEAGPLRAQEERDSASEVGVDIVEFLDYDDGVVV
                     YGTDLRRDIARVIRHRRPQVIVTLTHDESFAGGMTNQADHRAVGLAAIDAARDAANRW
                     IFRELLEQEGLEPWGGITAVLSSAANGPTHYVDVTGHLEPAIASLEAHHLYNTNLPDD
                     FPKPRDLVTGILTGTAAALGIEGVEHALTFRRYL"
BASE COUNT          610 a         1113 c         1348 g          524 t
ORIGIN      
        1 cgtagcgggg gaggtagcgc gggacgatgt tcagcagccc ggccatcgcc gacgctccgg
       61 cgaaccacag aatgccgatc gtcgagatgt cgtaggccgt gccgaagacg tcaccgagca
      121 ggtcgtggtc gagataggcc agggcccggc cgttggcctc accgccgggc tggaagtcgg
      181 ccgcgggaat caacaacgtc gtcaccagcg acgagcccag caacatgacg ctcatgatga
      241 gcgcggccgt cgtcagcagt ttgcgggcgt tgcggatccg gccggtcgga gcggcgggcg
      301 tgtcggtggg gtcgcccttg accagcggca tgacgacaac gcccgtctcg aagcccgaca
      361 gacccagagc cagcgccggg aagaccagca gggccgcccc gatgaccgcc atcggcgacg
      421 cataggtgga cgtgatggcg ctcgtccact cgccgacgtg ctccgagtgt gcggcgacct
      481 ccatgagacc gcgcccgacg acgaccgcag acagaccgag gtatgccgcg accagcacga
      541 ccgcgatccc gatggcttct ttgaagccct tgaggaagat ggcaccgagg aaggcgagca
      601 ggaccaacgt gacggtgacc tgctgaccgc tgaggacgct cttgaggaac gggttctcga
      661 tgaggtgagc gctcgcgtcg gccgccgaca gggtgatcgt gatgacaaag ccggtcgcga
      721 cgaagccgat gagcacgagg acgagcagtt tgctcggcca ataggtgagc agccgttcga
      781 gcatcgacag gctgccatcg ccgtgcgggc tctcctgcgc gacccgccga tacatcggca
      841 gggcccccag cagggtgacg agcacgagaa cgagggtggc gagtggggcc agggctccgg
      901 ccgcaagcgc agcgatgccc ggctggtagc ccaaggtcga gaaatagtcg acgccggtga
      961 ggcacatgac ccgccaccac ggctgggtgt gctggtgctg ggcgacctcg ctctcttcct
     1021 cgtagaaccc gcccggctca cgatgcccgg cgtcgaggaa ccacgagagg aatgcattgc
     1081 gtcgctcggt tgccgcgttg gtgatgctcg tcacggcgtg agcgtaacgg tcaggcgcgc
     1141 ggcatacctg cagtgagcaa ggtatgccgc gcgcccgggg gcctgcgatg tggtgtggtg
     1201 ccgagggact acttcacctt gtcgatgcct tcgtgaccga cggtgagcat cgccccgccg
     1261 tcgtgggcgg cgcgcaggtc ggcgtgcgcg aaggcacggg ccaggtggta tgccgcgata
     1321 gtcacgatcg tgcccagcgc gatgccggcc agctcgaagg tgtcgctcac cttgagggcc
     1381 gtgttgccga tgccgaggat gatgccggcc gccaccggga cgaggttgat cgggttggcg
     1441 aagtcgacgt tgttctcttt ccagatcttg gcgccgagca ggccgatcat cccgtagagg
     1501 acgacggtga tgccgccgag gacgccgccc ggggtggccg cgacgagggc accgaacttc
     1561 ggcgagaggc cgaagatgat ggcgatgaag gccgcgacga cgtaggcggc cgtcgaatag
     1621 atccgggtgg cggccatgac gccgatgttc tcggcgtagg tcgtcgtcgg ggagccgccg
     1681 accgaggagg cgaccgcggt ggcgacaccg tcggccgcga tcgcgcgacc catgacgtgg
     1741 tcgaggtcgg caccggtcat ctcggcgacg gccttgacgt ggccggtgtt ctccgcgatg
     1801 agggcgatga tcgcgggaag gacgacgagg accgcggcga aggagatcgt cggggcgtgg
     1861 aggccgacga gttccttgcc gtcggcgccg gtggtcgtcg ccggcgggaa gcccagccac
     1921 ggcgagctcg cgaccccgtc gaggttgacc cgccagtgcg tcgtcacctt gccggcgccc
     1981 gcgtcgaagc tggtgatctg gccgaagccc cggtcgaaca gccaggacag cacgaagccg
     2041 aagaccaggc tgaggaagat cgagatccgc ccgaggaagc ccttgagcgc gaccgcgcac
     2101 acgatggcga aggtcatgac caacagggcg acccactggt cctgcggcca gtaaacgttg
     2161 gcgacgaccg gcgcgaggtt gaagccgatg agcatgacga ccgcgccggt gacgaccgga
     2221 ggcagcacct tgtggatgat gcccgagccg gccacatgga cgagcagacc gacgagcgcg
     2281 aggacgacac cgccgatgag gatggcgccg gtgacggtgc gggagtcggc gcccggctgg
     2341 gcccggatcg cggcggcgac cccgacgaac gacgccgacg tgccgaggta ggacggaacc
     2401 ttgccgctca ccatgagcag gaagatgatc gtcgcgatgc cgctcatcat gatcgccaac
     2461 tgagcgttga ggcccatgag cagcgggaac acgaaggtgg cgccgaacat cgcgaccacg
     2521 tgttgggcac cgagcccgat cgtcttgccc caggcgaggc gttcatcggg ggcgaccacg
     2581 tcgccggggg cgagggtgtg cccgtcgccc ttgagtttcc aactgaatgc gcccatggat
     2641 gctgggctcc ttcccgtcac gagatagtcg ttccggggtt tcaccctctc acgtccaggg
     2701 gtatgccgcg tgcctgccac gcggcatacc cctggggtgg gtgagcggca gggcgagcgg
     2761 gcagggcgag cgggcagggt gagcgggcgg gggtcagagg tagcggcgga aggtgagggc
     2821 gtgctcgacg ccctcgatgc ccagagcggc ggcggtcccg gtgagaatgc cggtgacgag
     2881 gtcgcggggc ttggggaagt catcggggag gttggtgttg tagaggtgat gggcctcaag
     2941 cgaggcgatc gccggctcga ggtgcccggt gacgtccacg tagtgggtcg gcccgttggc
     3001 ggcgctggac aggacggcgg tgatgccgcc ccacggctca aggccctcct gctcaagcaa
     3061 ctcgcggaag atccaccggt tggcggcgtc ccgcgcagcg tcgatcgcgg ccaggccgac
     3121 cgcccggtgg tccgcctggt tggtcatccc tccggcgaac gactcatcgt gggtcagagt
     3181 gacgatgacc tgcgggcggc ggtggcggat cacccgggcg atgtcgcgac gtagatcggt
     3241 gccgtagacg accaccccgt cgtcgtagtc gaggaactcc acgatgtcga cgccgacctc
     3301 gctggccgaa tcgcgctcct cctgggcgcg cagcggacca gcctcctcgg gggcgatgct
     3361 gtcgatgccg gcctctccgc gggtggccag caggtaggtc accgtcttgc cggccttggt
     3421 ccacttgtcg acggcggcgg cggtgccgta ctcgatgtcg tccgggtggg cgacgatgac
     3481 gagggcgctg tcgaaactct cgtcgagcgg ttgcaaggtg aggcggggcg gcataccgga
     3541 aggctcgctc atggacggac gatagcccgc ttcgggcccc cgatggcggt gatta
//
LOCUS       DAOMZY010000354         3566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_25193_length_3566_cov_4.835659, whole genome shotgun sequence.
ACCESSION   DAOMZY010000354 DAOMZY010000000
VERSION     DAOMZY010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25193_length_3566_cov_4.835659"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(244..1650)
                     /locus_tag="PLK64_15175"
     CDS             complement(244..1650)
                     /locus_tag="PLK64_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter family
                     protein"
                     /protein_id="HPV81135.1"
                     /translation="MSVSFVLEALVVLGSIVMGTRAGGVGVGLWGGLGTFVLVFIFGE
                     APGEPPAAAMAIILAVVTTAALMQAAGGIDWMVAVAAQVIEKRPKQITLIAPLTAFLF
                     SVGAGTSNIIYPLLPVIYDVSYKNGIRPSRPMSITVVQAGVALAASPVSAAMAAMLTL
                     TDVAPYDLGLAQILAITFPACIIGIIVTSLVVNRMGLELADDPEVQARIASGQLAPPG
                     IDGAGAGAVGAGGAGGGGAGAGGESAAAKLTYTEAGRNSAIAFLLGVVAIVAFGIFPG
                     LRPSFEVGGKTSSVDMTSTIVLVMFVVGVLILFIGKPTVGTVPSQSVFKAGMISAIAL
                     YGIAWLTSTFIAAHEAFILKSIGTWVGSWPFIFALAVFLVSALTTSQSTATRTIVPIG
                     LAAGLPVGVVTGMWAGALGGVYTLPANGTQIAAANFDLTGTTKLGGKLFDHSFFVPML
                     VLSVVTIIVGAAIGLLLF"
     gene            1913..>3566
                     /locus_tag="PLK64_15180"
     CDS             1913..>3566
                     /locus_tag="PLK64_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81136.1"
                     /translation="MSLSLVTGHIRRQRVRRVVPRPDVERRLDEVPAGGLGVLIAPTG
                     SGKSVLVSQWLASTPGQRVAVLTLAARHTDIAVLARDLVATIRSVAPQVDPRLAGLAA
                     GGHATLGHPLVDALLDGLAAAPHDSVLVLEDAHALSNHPVIGDLGHLFTALPPTARGV
                     VAMRSDPPWSLHQLRLDDRLVEIRGADLAFAEADAQALLEDVSRTSLTDAQVALLVER
                     TDGWAAGLQLAAISLRTSRDIPGFVESFAGSDHLVSEYLLHEVIDEQEPAVRQFLLQT
                     SVLDWLSADLCDAVTGLENAAGMLDGLFHRSMFLIPLDEAGTTFRYQHLFADALRSRL
                     RIENPHSVSALHHRAARWLLAHGHEEEAVGHLLDAGDPAGAFRVISRIGHHFVERGEA
                     ATLVRWLSTIAAEDPDGPVEVEISLLAAQLAAGEGTAATETHRRLSRRPDLTLGERTA
                     ADALSAFLVFRDLPPPQVLQVTDRVRVALQGLDSDEVVDFLGIGGADSVQAVAEYTSG
                     LAHFVAGDLDRAAAVLERARTLPGTRYVVWHVYVISTLALVCA"
BASE COUNT          572 a         1253 c         1191 g          550 t
ORIGIN      
        1 gctcgacaaa gtgatgtgct caggtgagta ggaccattcc gcttggcgtg gtcgcgacga
       61 cccgcacgct gcgggtgggt ggcaccggtg ttgcgctgcc cctcattggc caagccgaat
      121 gtgggcgtcg gataagcgac cgggaagggt gccacgcgag gctacgcgaa ggtcgccgcg
      181 gctgctttgg cggcatcggc ctcgccggtc ggcgacgggg agtggcgggc agagaagacg
      241 ccgtcagaac agaagcaggc cgatggctgc cccgacgatg atcgtgacta ccgacagcac
      301 gagcatgggc acgaagaagc tgtggtcgaa caacttgcca cccagcttgg tcgtccccgt
      361 gaggtcgaag ttggccgccg cgatctgggt gccgttggcc ggcagcgtgt agacgccgcc
      421 cagggcaccg gcccacatcc ccgtcacgac tccgaccggc agacctgctg ccaggccgat
      481 gggcacgatg gtccgcgtgg cggtggattg actcgtcgtc agggccgaca cgaggaagac
      541 ggcgagggcg aagatgaagg gccaggaccc cacccacgtg ccgatcgatt tgaggatgaa
      601 ggcctcgtgg gcggcgatga aggtcgaagt gagccaggcg atcccataga gggcgatcgc
      661 cgagatcatc ccggccttga agaccgactg gctgggcacc gtgcccaccg tcggcttgcc
      721 gatgaagagg atcagcacgc cgaccacgaa catcaccaac acgatcgtgc tcgtcatgtc
      781 gactgacgag gtcttgccgc cgacctcgaa agacggccgc aggcccggga agatcccgaa
      841 cgcgacgatg gccacgaccc ccagcaggaa cgcgatcgct gagttccgcc ctgcctcggt
      901 gtaggtcagt ttggccgccg ccgactcgcc gcccgcaccc gcgccgcccc cacctgcgcc
      961 acccgcaccc acggcacccg cgcccgcgcc gtcaatccca ggcggcgcca actgtccgga
     1021 agcgatcctc gcctgcacct cgggatcgtc cgccagctcc aaccccatcc gattcaccac
     1081 gagcgacgtc acgatgatgc caataatgca ggcggggaac gtgattgcga ggatctgagc
     1141 cagccccagg tcatagggcg cgacgtcggt caaggtcaac atggccgcca tcgctgccga
     1201 cacgggcgag gccgccaaag cgactcccgc ctgcaccacc gtgatggaca tgggtcgcga
     1261 gggacgtatg ccgttcttat acgacacgtc gtaaatgacc ggcaacagcg gatagatgat
     1321 gttgctcgtc cccgcgccga cggagaagag gaacgccgtc aggggagcga tgagggtgat
     1381 ctgcttgggg cgcttctcga tgacctgggc cgcgacggcg accatccaat cgatcccccc
     1441 ggccgcctgc atgagggcgg cggtcgtgac cacggccagg atgatcgcca tggcggcggc
     1501 cggcggctcg ccgggcgcct caccgaagat gaagacgagg acgaaggtgc cgaggcctcc
     1561 ccagaggccc acgcccaccc caccggcccg agtgcccatg acgatggagc cgagaacgac
     1621 cagggcctca aggacgaagc tgacgctcat ggcgtctcct tccgcgcgcg gagtggagca
     1681 ctcaggaacc cgtccgtcga ccccgagtac tgcgaacgta tccgcgggcc gtggagggcc
     1741 tcgtcaccca cgaagtgtga gttccacgcc cggaaaggcg tcgcttcacc ctccaggccc
     1801 gcggccgccc ccgaccgcga tccggtatgc cgcccgcccc cgttcgcggc gtcgggaccg
     1861 aaaccgtcgg atcgagtcgc agtcggaggc ctctgctggc agcatttccg atatgtcact
     1921 ctccctggtg actggacata ttcgacggca acgcgtgcgc agggtcgtgc cgcggccgga
     1981 cgttgagcga cgtctcgacg aggttccggc cggagggctg ggtgtcctga tcgcgccgac
     2041 cgggtcaggc aagtcggtgc tcgtcagtca gtggttggcc tccacccccg gccaacgggt
     2101 ggccgtgctg accctggccg cgcggcatac cgacatcgca gtccttgccc gcgacctcgt
     2161 ggcgacgatc cgctctgtgg cgccgcaggt cgatccgcgc ctcgctggcc tcgccgcggg
     2221 cgggcacgcg accctcggcc accccctcgt cgacgccctg ctggacggac ttgccgcggc
     2281 accacacgac agcgttctcg tcctcgagga cgcccacgcg ttgtccaacc accccgtgat
     2341 cggcgacctg gggcacctct tcaccgccct gccgccgaca gcccgcgggg tcgtggcgat
     2401 gaggagtgac ccgccctggt ctctgcacca gcttcggctg gacgaccggc tggtcgagat
     2461 ccgcggggcc gatctggcgt ttgcggaggc cgacgcccag gccttgttgg aggacgtgtc
     2521 gcgaacctcc ctcaccgatg cgcaggtggc gctcctcgtc gagcgcaccg acgggtgggc
     2581 ggcggggctg cagttggccg cgatctcgtt gcgaacctct cgggacatcc cggggttcgt
     2641 cgagtcgttc gccggcagtg accacctggt ctcggagtac ctgttgcacg aggtgatcga
     2701 cgagcaggag ccggccgtgc gccaattcct cctgcagacc tccgtcctgg actggttgtc
     2761 ggccgatctg tgcgacgccg tcacgggcct ggagaacgcc gcgggaatgc ttgacgggct
     2821 ctttcaccgg tcgatgtttc tcatccccct ggacgaggcc ggcacgacct tccgctatca
     2881 gcacctcttc gccgacgcgc tgcgatcccg gctgcggatc gaaaacccgc actcggtgag
     2941 cgccctgcac caccgggccg cccgctggct gttggctcac gggcacgagg aggaagcggt
     3001 cggccatctg ctcgatgccg gcgacccggc cggggcgttc cgagtcatct cccgcatcgg
     3061 ccaccacttc gtcgaacggg gtgaggccgc aaccctcgtc cgttggctga gcaccatcgc
     3121 tgccgaggat cccgatggcc cggtcgaggt cgagatcagc ctgctcgccg cccagttggc
     3181 tgcgggtgag gggacggcag cgaccgagac ccatcgccgg ctcagccgcc gtcccgatct
     3241 caccctgggg gagcggaccg cagccgacgc gctctctgcc ttcctggtct tccgcgacct
     3301 gccgccgccg caggtcctgc aggtcactga tcgggtgcgc gtcgccctgc aagggctcga
     3361 ctctgatgag gtcgtcgact tccttggcat cgggggagcc gactccgtcc aggccgtcgc
     3421 cgaatacacc tcgggtctgg cgcatttcgt cgccggcgac ctcgaccgcg ccgccgccgt
     3481 gttggagcgc gctcggacac tgcccgggac ccgctacgtc gtctggcacg tctatgtgat
     3541 cagcaccctg gccctggtct gcgcct
//
LOCUS       DAOMZY010000355         3558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_25268_length_3558_cov_7.500428, whole genome shotgun sequence.
ACCESSION   DAOMZY010000355 DAOMZY010000000
VERSION     DAOMZY010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25268_length_3558_cov_7.500428"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..802
                     /locus_tag="PLK64_15185"
     CDS             146..802
                     /locus_tag="PLK64_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81137.1"
                     /translation="MTRPTPFDLKEPDLMVQLVTRWAIRLVVVIAAVVVALAGFTMVK
                     DKGLLSPFGINSEGQDTQVIQAVQRVQEVALLKLVIQGITSEKAERELFGKSIPGTGQ
                     SVFLQYDFNAKLGIDGEDVTVSKTGEHTYLVTIPTFMVIGYDDPTFKVATEDGGILSW
                     VTTDIDKVDMVNEVFDEAAQKQYLEEQQDQLEDQAELFYSSLITSIDPEAVTTFEFRG
                     "
     gene            complement(799..1179)
                     /locus_tag="PLK64_15190"
     CDS             complement(799..1179)
                     /locus_tag="PLK64_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81138.1"
                     /translation="MTEPGAAAASLIWATCGRRWGFRFLLTAGLPDPLATYERAFVVL
                     GDESQGCHREGELVALRFSDPLGRRDTAGRVIPHDFVVFGDAAKGVASVEDGLRLVWP
                     VVADVFAQVWDADGPPSAADLFAG"
     gene            complement(1176..2366)
                     /locus_tag="PLK64_15195"
     CDS             complement(1176..2366)
                     /locus_tag="PLK64_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HPV81139.1"
                     /translation="MAKSPRVREQHIAVFGERGSGKTVLVSSFFGPTQEGSYSNELWN
                     LIADETGQGNRLYQNYLGMRDRATAPATTKFASTTYYFTLKLKSGDNSASKRRPFDAL
                     RLAWHDYPGEWFEETPSSDEEAQRRVDTFRSLLRSDVAVFLVDGQKLVDYQGEEERYL
                     KSLFTNFRQGLLRLKDDLLVDDARLVEFPRIWILALSKADLFPDWDVYAFRDLVIGKA
                     AGDIDRLRETIEEFVDTPQALSVGEDFMLLSSAKFELTPTGSELGEIDVTQRVGLDLI
                     MPVASLLPLERRVLWNERLEIPRKVLDAMADGAEDIAAALKGPLGIGLVKVLSKLPKG
                     GRVASAAAVPVFLAAADAAGPKLKELNVQARANLDYLTATLTQFKLDLDQGVKDRLLI
                     RSLK"
     gene            complement(2459..>3558)
                     /locus_tag="PLK64_15200"
     CDS             complement(2459..>3558)
                     /locus_tag="PLK64_15200"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPV81140.1"
                     /translation="RTDGVDGSALRGGDEPVRSHSALYVDVGYLLASAATRVTGTSLR
                     SGVNVDYGRLIDALIAQVEDHSGLPVLRVNWYDSGGRPGGQADATQERIGMLPRVKLR
                     LGRLSYAGEQKGVDVRLGLDLATHGRNRVVDIVYLVSGDDDLTEAVEEAQGHGAQVVV
                     LAVPDRAGSAYGVSRHLQREADDLVLIDGQAIDAAVHKPMPVPAVSAPAVDQHGEPHS
                     TVEAVVEAEVEVRPATTAGPTPADLVRATARTARPPAARVAAPTSVMAWSTSTGEVRP
                     EWLAVDDADGDQPDDVVDEVCRKVFTSWRLTASDSDLRVLLEGRPTIPGAIDRALLVD
                     LSGSTGVYDIDEPTRFRLRRHFWVVVDTEVR"
BASE COUNT          676 a         1171 c         1106 g          605 t
ORIGIN      
        1 gctccgaaga ctccgattcg catggggtcc agtctagaga gccgtccgga tgccggtcgc
       61 tcccgccacc atgcgggcgg cgcgctccct cgcgagaacc gagaacaggg aaccgcgcgt
      121 ctgtgattga ccgcaagcag atacggtgac gcgaccaacc ccgttcgatc tgaaggaacc
      181 tgacctcatg gtgcaactcg tgacgaggtg ggcgattcgc cttgtggtcg tcattgccgc
      241 cgtcgtggtg gctttggcag gtttcacgat ggtgaaggac aaggggctgc tctcgccctt
      301 cgggatcaac tccgagggcc aggacacaca ggtgatccag gccgttcaac gggtccagga
      361 agtcgccctc ctgaaactcg tcatccaagg gatcacctcg gagaaggctg agagggagtt
      421 gttcggcaag agcatccccg gtactggtca gtccgtcttc ctgcagtacg acttcaatgc
      481 gaagctcggg atcgacggcg aagacgtgac tgtcagcaag accggagagc acacgtacct
      541 cgtcaccatc ccaaccttca tggtcatcgg ctacgacgac ccgaccttca aggttgccac
      601 ggaggatggc ggcatcttga gctgggtcac cacggacatc gacaaggtcg acatggtcaa
      661 cgaggtcttc gacgaggccg cacagaagca gtacctcgag gagcagcagg accagctcga
      721 ggatcaggct gaactcttct actccagcct gatcaccagc atcgaccccg aagccgtgac
      781 gacattcgag ttccggggtt agccagcgaa caggtcggcc gcagacggtg ggccatcggc
      841 atcccacacc tgggcgaaga cgtccgcgac gactggccag acaagcctca gtccgtcctc
      901 cacggacgcc accccctttg ccgcgtcacc gaacaccacg aagtcgtgcg ggatcacccg
      961 ccccgcggta tctcgccggc ccaaggggtc tgagaatcgc agcgccacca actcgccctc
     1021 ccggtggcaa ccctgcgact cgtcccccag aacgacaaaa gcccgttcgt aggttgccag
     1081 gggatcggga agacccgcag tcagcaagaa gcggaagccc caacgacgcc cacacgtggc
     1141 ccagatgagc gacgcggcgg cagctccggg ctcagtcact tcagactccg gatgagcaag
     1201 cgatccttga ctccttggtc aaggtccaac ttgaactggg tcagggtcgc cgtcaggtag
     1261 tcaaggttcg cgcgcgcttg gacgttgagc tccttgagtt tggggcccgc tgcgtcagcc
     1321 gccgcgagga acacaggcac ggcagccgcg ctggcaacac ggccgccctt cggtagcttt
     1381 gacaacacct tgaccaagcc gatccccagg ggccccttca gcgctgccgc gatgtcctcg
     1441 gcaccatcgg ccatggcgtc gagcaccttc cgcgggatct ccaagcgttc gttccacaga
     1501 acccgtcgct ctagcggcaa cagcgacgcc accggcatga tcaggtcaag gccgacgcgc
     1561 tgagtcacgt caatctcccc gagctccgag cccgtcggag tcagttcgaa cttcgccgac
     1621 gacagcaaca tgaagtcctc gccgacggac aaggcctggg gcgtgtccac gaactcctcg
     1681 atggtttcgc gaaggcggtc gatgtcgccg gcggccttac cgatgacgag gtcacggaag
     1741 gcgtagacgt cccagtctgg gaacaggtcc gccttcgaca gcgccagaat ccagatccgc
     1801 gggaactcga ccaatcgggc gtcgtcgacg agcagatcat ccttgaggcg cagcaggccc
     1861 tgacggaaat tggtgaacag cgacttgagg tatcgctctt cctccccttg gtagtcgact
     1921 agcttctggc cgtcgacgag aaagacggcc acatctgaac ggagcaggga tcggaaggtg
     1981 tccacgcgcc gctgcgcttc ctcgtcactg ctcggcgtct cctcgaacca ctccccgggg
     2041 tagtcgtgcc acgcgagtcg caacgcgtcg aaggggcgcc gctttgaggc tgagttgtca
     2101 ccactcttca gcttgagcgt gaagtagtag gtggtgctcg cgaacttcgt cgtggcaggg
     2161 gcggtggcac ggtctcgcat gccgaggtag ttctgataca gccgattgcc ttggccggtc
     2221 tcatcggcga tgaggttcca cagctcgttg gagtacgacc cttcttgagt tggtccgaag
     2281 aaggacgaca ccaagacggt cttgcctgag cccctttccc caaaaaccgc gatgtgctgc
     2341 tctcggaccc gcggggattt cgccatgccg cgaaggtacc cgatcgagtg tcagggtgcg
     2401 gtgagcaggt ggcgctgact ccacactcca cgagagcctg caagccgatc accgaccctc
     2461 atcgaacctc cgtgtcgacg acgacccaga agtgccgtcg taggcgaaag cgagtgggct
     2521 catcgatgtc atacacacca gtcgagccgg acaagtcgac gagcagagcc ctgtcgatcg
     2581 caccgggaat ggtgggccgc ccctcgagca gcacccggag gtcagagtcg gatgccgtga
     2641 gtcgccagga tgtgaagact tttcggcaca cctcgtccac gacatcgtcg ggctggtcgc
     2701 cgtctgcatc gtccacggcc agccactcgg ggcggacttc tcccgtcgat gtggaccacg
     2761 ccatcacgct ggtcggagcc gccacccgag cagccggtgg acgggctgtt cgcgctgtgg
     2821 cgcgtacgag gtcagccggt gtgggtccag cggtcgtcgc cggcctcacc tccacctcag
     2881 cctcgacgac cgcctcaaca gtgctgtgcg gctcgccgtg ctgatcgaca gccggtgcgc
     2941 tgaccgcggg gaccggcatg ggcttgtgca ccgccgcgtc gatcgcctgc ccatcgatga
     3001 gcacgaggtc gtcggcttcc cgctgaaggt gcctgctgac cccgtatgca gaaccggcgc
     3061 gatcagggac ggcgagaaca accacctggg cgccgtgacc ctgcgcctcc tcgacggcct
     3121 cggtcaggtc gtcgtcgccg gagaccaggt agacgatgtc gacgacgcga ttgcgcccat
     3181 gagtggcgag gtcaagaccc agacgcacgt cgacgccctt ctgctctccg gcgtacgaca
     3241 gtcgaccgag tcgcagtttc acccggggca acattccgat ccgctcttgc gtggcgtcag
     3301 cctgcccgcc gggtcgccca ccggagtcgt accagttgac acgcaacacg ggcagcccgc
     3361 tgtggtcctc gacttgtgcg atgagggcgt cgatcaagcg tccgtagtcg acgttgaccc
     3421 cgctgcgcag ggaggtaccg gtgactcgcg tcgccgcgga agccagcaga tagccgacgt
     3481 cgacgtagag ggcgctatgt gacctcacgg gttcatcccc tccgcgtagc gcggagccat
     3541 cgactccatc tgttcgat
//
LOCUS       DAOMZY010000356         3534 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_25509_length_3534_cov_5.363323, whole genome shotgun sequence.
ACCESSION   DAOMZY010000356 DAOMZY010000000
VERSION     DAOMZY010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25509_length_3534_cov_5.363323"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..461
                     /locus_tag="PLK64_15205"
     CDS             240..461
                     /locus_tag="PLK64_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HPV81141.1"
                     /translation="MTEPLPRPDSQDPGAAFAAVVALRRKANRLEVDTVVEAIGQGWT
                     WAAVGEALGISAQAAHKKFAATVRARAAR"
     gene            533..1054
                     /locus_tag="PLK64_15210"
     CDS             533..1054
                     /locus_tag="PLK64_15210"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HPV81142.1"
                     /translation="MGLFNAGRDVRTINALLTGAEHEARRGGDALPGPEHLLLSALNL
                     ADGTAALALGRFGVDGAAVHAALERVHADALSDIGVSEPDPAAEAGADEPLGRAGGVF
                     RSTPQAQQVFRDAVALSKQASPSRLLGAHVVAAVCAVEHGTSARVLAALGIDRSELRT
                     AALTQAAGDGSRR"
     gene            complement(1115..3454)
                     /locus_tag="PLK64_15215"
     CDS             complement(1115..3454)
                     /locus_tag="PLK64_15215"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HPV81143.1"
                     /translation="MTDQVARTDPALATVTVPGAGLTGAPPTGPQPRLRDRLARLGTP
                     RAPVNMALEPILQSLREHHPKAEVALLQRAFEVAERAHQGQTRRSGDDYITHPLSVTQ
                     ILADLGLTPPTLAAALLHDTVEDTDYSLEDLRRDFGGEIAQLVDGVTKLDKVTWGEAA
                     KAETVRKMVVAMARDIRVLVIKLADRLHNARTWKYVSRESASAKAQETLEIYAPLAHR
                     LGMNTIKWELEDLAFQALYPKMYDEIVDLVAARAPAREEFLARVCSEVAEDLKVAGIK
                     ATVTGRPKHYYSVYQKMIVRGRDFEEIYDLVGVRVLVETVRDCYGALGSLHARWNPVP
                     GRFKDYIAIPKFNMYQSLHTTVIGPQGKPVEIQIRTHTMHRRAEYGVAAHYKYKEDHK
                     RGGRGAPVESDSLAETAWLRRLLDWQREIADPGEFLDSLRFDLGGGEVFVFTPKGAVV
                     SLPAGSTPVDFAYAVHTEVGHHCIGGRVNGRLAPLDSVLENGDSVEILTSKADGAGPS
                     RDWLAFVKSPRARNKIRQWFTKERREEAIEQGKDAIAKYLRKQGLPLHRLMSAESLTA
                     VAGDLRYSDIDSLFAAVGDSNVSAQHVVSRLVATLGGEEATSEDLAEATVPSRARSRV
                     RSGDPGVVVVGTDDVWVKVARCCTPVPGDAIIGFITRGNGVSVHREDCTNADALRREP
                     DRLIEVAWDPSAASVFLVQLQVEALDRNRLLSDITRAISDHHVNILAASVQTTRNRVA
                     TSRFTFEMGDPGHLDHVIAGILKVEGVYDVYRITGTKRD"
BASE COUNT          523 a         1179 c         1263 g          569 t
ORIGIN      
        1 ccgcacaggc atccggcaac gccaagaagg tggccgacgc ccaagccgca ctcgatgccc
       61 gtcgccagtg gctcgaccag gcccgttcgg gtctggcgga gtttggcggc cgctgaccca
      121 cgcgcgccag cgacgcggca tacccaggcg gggtatgccg cgtcgttgcg ttaggggccg
      181 tctccccatc gtcatcaact caggttgaca cccaggtgtt atcaacgtag attgatggca
      241 tgacggagcc actgcctcgc cctgactcgc aggatcctgg ggccgcgttc gcggcggtcg
      301 tcgccttgcg ccgcaaggcc aatcggctcg aggtcgacac cgtggtcgag gcgatcggcc
      361 agggctggac ctgggccgcc gtgggcgagg cgctgggcat cagtgcccaa gcggctcaca
      421 agaagttcgc ggccacggtc cgggccagag cggcccgctg atccactggc gatccgccag
      481 gcgcccgctg acgaatgccg tcggcactat gaccacacac aggaggacaa cgatgggact
      541 gttcaacgcc ggccgggatg tgcgcacgat caacgccctg ctcaccgggg ccgaacatga
      601 ggcgcgacgg ggcggggacg cgctccctgg gccggagcac ctcttgctgt ccgcactcaa
      661 tctggcggac gggaccgcgg ccctggccct cgggcgtttc ggcgtcgacg gtgcggccgt
      721 gcatgccgca ttggagcgtg ttcatgccga tgccctctca gacatcggtg tgtcggagcc
      781 cgacccggcc gcggaggcgg gcgctgacga gccgttggga cgcgcgggcg gtgtcttccg
      841 ctcgaccccg caggcgcagc aggtgttccg cgacgcggtg gccctatcga agcaggcctc
      901 gccatcccga ctgctcggtg cgcacgtcgt ggcggccgtc tgcgcggtcg agcacggcac
      961 ctcagcccgc gtcctggccg ccctagggat cgaccgcagc gagttgcgca cggcagccct
     1021 gacgcaggct gccggcgatg ggtcccgtcg ctgacccgag agccgacatg cgagaggcga
     1081 gcgcgtcgcg tcgtcgcgag cgctcgggca gctctcagtc gcgcttggtg ccggtgatcc
     1141 ggtagacgtc gtagacgccc tcgaccttga ggatgccggc gatgacgtgg tcgaggtgcc
     1201 ccgggtcgcc catctcgaag gtgaagcgtg aggtcgcgac ccggttgcgg gtggtctgca
     1261 cggatgcagc gaggatgttg acgtggtgat cggagatcgc ccgggtgatg tccgacagca
     1321 gccggttgcg gtcgagggcc tcgacctgca gttgtacgag gaaaacgctc gcggctgacg
     1381 ggtcccaggc gacctcgatg agccggtccg gctcgcgccg cagtgcgtcg gcattggtgc
     1441 agtcctcgcg gtggaccgac acgccgttcc cacgggtgat gaagccgatg atggcgtcgc
     1501 cgggcaccgg ggtgcagcag cgggcgacct tgacccacac gtcgtcggtg ccgacgacga
     1561 cgacgcccgg gtcgccgctg cgcacccgac tgcgggcacg cgacggcacg gtggcctcgg
     1621 cgaggtcctc gctggtcgcc tcctcgccgc cgagcgtggc cacgagccgg gacacgacat
     1681 gctgggccga gacgttcgag tcgccgacgg ctgcgaagag gctgtcgatg tcggagtagc
     1741 gcaggtcgcc ggcgacggcg gtcaggctct cggcggacat caaccgatgc agcgggagac
     1801 cctgcttgcg caggtacttg gcgatcgcat ccttgccctg ctcgatcgcc tcctcacgcc
     1861 gttccttggt gaaccactgg cggatcttgt tgcgggcgcg agggctcttg acgaacgcga
     1921 gccagtcccg cgacggtccg gcgccgtcgg ccttcgaggt gaggatctcg accgagtcgc
     1981 cgttctccag gaccgagtcc aagggcgcca accgaccgtt gactcgacca ccgatgcagt
     2041 ggtgcccgac ctcggtgtgc acggcatacg cgaagtccac gggagtcgac ccggcgggaa
     2101 gtgagacgac cgcgcctttg ggggtgaaga cgaagacctc gccgccgccg aggtcgaagc
     2161 gcagcgagtc gagaaactcc cccgggtcgg cgatctcgcg ctgccagtcg agcaggcggc
     2221 gcagccaggc ggtctcggcg agcgagtcgg actcgacggg agcgccgcgg ccgccgcgct
     2281 tgtggtcttc tttgtacttg tagtgcgcag ccacgccgta ttcggcccgt cggtgcatcg
     2341 tgtgggtgcg gatctggatc tcgaccggct tgccctgcgg cccgatgacg gtggtgtgca
     2401 gggactggta catgttgaac ttcgggatcg cgatgtagtc cttgaaccgg cccggcaccg
     2461 ggttccaccg cgcgtgcaat gacccgagcg cgccgtaaca gtcgcgcacc gtctcgacga
     2521 gcacccgcac gccgaccagg tcgtagatct cctcgaagtc gcgcccgcgc acgatcatct
     2581 tctggtagac ggagtagtag tgcttgggcc gaccggtgac cgtcgccttg atcccggcca
     2641 ccttgaggtc ctcggcgacc tccgaacaga ccctggcgag gaactcctcc cgcgcggggg
     2701 cgcgagcggc cacgaggtcg acgatctcgt cgtacatctt ggggtagagc gcctggaagg
     2761 ccaggtcctc caactcccac ttgatggtgt tcatgcccag ccggtgcgcc agcggggcgt
     2821 agatctccag ggtctcctgg gccttggcgg acgccgactc tcgggagacg tatttccagg
     2881 tgcgggcatt gtgcagccgg tcggcgagct tgatgacgag cacccggatg tcacgggcca
     2941 tcgcgacgac catcttgcgg accgtttcgg ccttggccgc ctcgccccag gtcaccttgt
     3001 cgagcttggt cacgccgtcg accagttgcg cgatctcgcc accgaagtcg cggcgcaggt
     3061 cctcgaggct gtagtccgtg tcctccaccg tgtcgtgcaa cagcgccgcc gccagcgtgg
     3121 gcggggtcaa cccgaggtcg gcaaggatct gggtcaccga cagcgggtgg gtgatgtagt
     3181 cgtcgccgct gcgcctggtc tgcccctgat gggctcgctc ggcgacctcg aaggcccgct
     3241 gcagcagcgc gacctcagcc ttggggtggt gctcacgcag gctttgcagg atcggctcga
     3301 gggccatgtt gacgggagct cggggtgtcc ccaaccgcgc caggcgatcg cgcaacctcg
     3361 gctgtgggcc ggtcggaggc gcacccgtga gtccggcgcc ggggaccgtg acggtcgcca
     3421 gggccgggtc ggtgcgtgcg acctggtcgg tcatgtccga agtgtagtca cccatgacga
     3481 cctctcccgc cgccctccgc cgggtgggag tcagtagacg aggagagaga cgac
//
LOCUS       DAOMZY010000357         3526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_25601_length_3526_cov_5.900029, whole genome shotgun sequence.
ACCESSION   DAOMZY010000357 DAOMZY010000000
VERSION     DAOMZY010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25601_length_3526_cov_5.900029"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PLK64_15220"
     CDS             complement(<1..586)
                     /locus_tag="PLK64_15220"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV81144.1"
                     /translation="MSDLLDDAVGALRGWLLWWWPTSAHGDVPAPEEFRLRDGTRVWI
                     RPILPSDRELHAVNYERLSADSKFHRFLTPMTHLSEEMLTRLVDDVDGVDHVAYYVFG
                     DSDDPEVSMLPIAIGRIKRDPERPDTADVAVTVMDPWHGRGVATALLPVLVARRPEGV
                     TGIVTAVATDNPASIAMLKRLGPTEVIPMGGGALE"
     gene            674..1978
                     /gene="paaF"
                     /locus_tag="PLK64_15225"
     CDS             674..1978
                     /gene="paaF"
                     /locus_tag="PLK64_15225"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009882061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HPV81145.1"
                     /translation="MTTDLRDEGERWSLDELRVRQLERLKDTVHRVYANVPHYKKSWD
                     EAGVHPDDIRELSDLAKLPFTDKLTLRDNYPFGLFAVPREQVVRIHASSGTTGKPTVV
                     GYTREDIDMWAGVMARSIRAAGGRPGDVLHVAYGYGLFTGGLGAHYGAEKLGCTVVPI
                     SGGMTERQVQLIQDFGCKIMMVTPSYFLSVLDEFERQGIDPASTTLKIGIFGAEPWTP
                     AMRTEIESRCHMDAVDIYGLSEVVGPGVSCESAETKDGLHIWEDHFYPEIIDPLTGEV
                     LPDGEQGELVFTSLTKQAFPVIRYRTKDLTRLLPGTAFPTMRRMEKVTGRTDDMMIIR
                     GVNVFPTQIEEIILAEPGLAPYFQCVLTQPGNMLELTVLAESTKTLGDADRVELEKRI
                     GKRIKDNIGTTAKIEVRAPGGIERSVGKARRIVDTRKSPFPT"
     gene            2091..3401
                     /gene="paaF"
                     /locus_tag="PLK64_15230"
     CDS             2091..3401
                     /gene="paaF"
                     /locus_tag="PLK64_15230"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009379670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HPV81146.1"
                     /translation="MATASVYSPDADAGLHWSLDELRARQLERLQATVRRVYAAVPHY
                     RRVFDEAGVHPDDIRTLADIAKLPFTDKSTLRDNYPFGLFAVPREQVSRIHASSGTTG
                     KPTVVGYTAGDLDMWTNLMARSLYAAGVRTGDVVHNSYGYGLFTGGMGYHYGAERLGC
                     TVVPIAGGMTERQVQLIRDFEPRATLVTPSYFLSILDEMDRQGIDPRESSLRIGVFGA
                     EPWTPAMREAVETRSSISAIDEYGLSEVIGPGVAGESAETKDGLNIWEDCFYPEIIDP
                     VTGEVLPDGSEGELVFTSLTKEAFPVIRYRTKDLSRLMPGTAYPTMRRMAKVTGRTDD
                     MMIIRGVNVFPTQIEEIILREPGLAPYFQCVLTQPGNMVELTVVVETLVHEGEERANS
                     IAADISHEIKARIGTSAKVEIRDPGGIERSVGKAKRIVDLRPKG"
BASE COUNT          623 a         1238 c         1123 g          542 t
ORIGIN      
        1 cctcgagcgc tccaccgccc atcggaatga cctcggtggg gcccaaccgc ttcaacatgg
       61 cgatcgaggc cgggttgtcc gtggcaaccg ccgtgacgat gccggtgaca ccctcgggac
      121 gacgagccac cagcacgggc aacagcgctg tcgccacacc gcggccgtgc caagggtcca
      181 tcaccgtgac tgcgacgtcg gcggtgtccg gtcgctccgg gtcacgcttg atccgcccga
      241 tagcgatggg cagcatcgac acttccgggt cgtcgctgtc gccgaacacg tagtaggcca
      301 cgtggtccac gccgtcgacg tcatcgacga ggcgggtgag catctcctcg gacagatgcg
      361 tcatcggagt gaggaaccgg tgaaacttcg agtcggccga cagccgctcg tagttcacgg
      421 catgcagttc gcgatcgctg ggcagaatgg gacgaatcca cacccgagtc ccgtcgcgca
      481 agcggaactc ctcgggcgcg ggcacgtcgc catgggcgct cgtcggccac caccacaaca
      541 gccagccccg cagcgccccc accgcgtcat ccaagagatc tgacatctca gggcaagggt
      601 atccaacccg gtgtggcgcg aacgacagac gcgttgtgtt gtgcaccgca tacctcatag
      661 acagaatcac cccatgacga ccgacctcag agacgagggc gagcgctggt cactcgacga
      721 gcttcgagtc cgccagttgg agcgcctcaa ggacactgtc caccgcgtgt acgcgaacgt
      781 tccccactac aagaagtcgt gggacgaggc gggcgtccac cccgatgaca tccgcgaact
      841 gtccgacctg gccaagctcc cgttcaccga caagctgacg ttgcgcgaca actacccgtt
      901 cgggttgttc gccgtgcctc gtgagcaggt cgtccggatc cacgccagca gtggcaccac
      961 cggcaagccg accgtcgtcg gttacacccg cgaagacatc gacatgtggg ccggggtcat
     1021 ggcccgctcc atccgggccg ccggcggtcg tcccggtgac gtcttgcacg tcgcctacgg
     1081 ctatggcctg ttcaccggtg gcctcggtgc ccactacggc gccgagaagc tcgggtgcac
     1141 cgtggtcccg atctccggcg gcatgaccga gcgccaggtc cagctcatcc aggacttcgg
     1201 ctgcaagatc atgatggtca cgccgagcta cttcctctca gtcctcgacg agttcgagcg
     1261 ccagggcatc gacccggcct ccacgaccct gaagatcggc atcttcggtg ccgagccgtg
     1321 gacccccgcc atgcgcaccg agatcgaatc ccgttgccac atggacgcgg tcgacatcta
     1381 cggtctgtcc gaggtggtcg gccccggcgt ctcctgcgag tctgccgaga cgaaggacgg
     1441 cctgcacatc tgggaggacc acttctaccc cgagatcatc gatcccctca cgggcgaggt
     1501 cctccccgac ggcgagcagg gcgagttggt cttcaccagc ctcaccaagc aggccttccc
     1561 ggtcatccgc taccgcacca aggacctcac ccggctgctg cccggcacgg ccttcccgac
     1621 gatgcgccgg atggagaagg tcaccggtcg caccgacgac atgatgatca tccgcggcgt
     1681 caatgtcttc ccgacccaga tcgaggagat catcctcgcc gagcccggcc tcgcgcccta
     1741 cttccagtgc gtcctgaccc agcccggcaa catgctcgag ctcaccgttc tcgccgagag
     1801 caccaagacc ctcggcgacg ccgaccgggt cgagttggag aagcggatcg gcaagcggat
     1861 caaggacaac atcggcacca ccgccaagat cgaggtccgc gcaccgggcg gcatcgagcg
     1921 ttcggtcggc aaggcccgcc ggatcgtcga cacccgcaag tccccgttcc ccacctgaac
     1981 gacccgcgcg agcccaccga ccgcgtcacc aacccagccg cattcggtat gccgcgcatc
     2041 gccccctgag gcggtgcgcg gcataccgtg cgccgaagag gagcaccccg atggcgaccg
     2101 cttccgtcta ctcgcccgac gccgacgccg gcctgcactg gtctctcgac gagctgcgcg
     2161 cgcgtcaact ggagcggctg caagccaccg tgcggcgggt gtatgccgcc gtcccccact
     2221 accgccgcgt gttcgacgag gcgggggtgc accccgacga catccggacg ctcgccgaca
     2281 tcgccaaact gcccttcacc gacaagagca cgctgcgcga caactacccg ttcgggctgt
     2341 tcgccgtgcc gcgcgagcag gtgtcgcgca tccacgcgag cagcgggacg accggcaagc
     2401 cgaccgttgt cggctacacc gcgggggacc tcgacatgtg gaccaacctc atggcgcgtt
     2461 ccctgtatgc cgcgggcgtc cggaccggtg acgtcgtgca caactcgtat ggctatgggc
     2521 tgttcaccgg tgggatgggc tatcactacg gcgccgagcg gctggggtgc acggtcgtgc
     2581 cgatcgccgg cgggatgacc gagcggcagg tgcagctcat ccgtgacttc gagccgcggg
     2641 ccacgctcgt gacgccgagc tatttcctgt cgatcctcga tgagatggat cgtcaaggca
     2701 tcgacccgcg tgagtcgagt ctgcggatcg gggtgttcgg cgccgagccg tggacgccgg
     2761 cgatgcgcga ggcggtcgag acgcgcagca gcatctcggc gatcgacgag tatgggctgt
     2821 ccgaggtcat cggccccggg gttgccggcg agtcggccga gaccaaggac gggctcaaca
     2881 tctgggagga ctgcttctat cccgagatca tcgacccggt gaccggcgag gtgctgccgg
     2941 atgggtccga gggtgagttg gtcttcacca gcctcaccaa ggaggccttc cccgtcatcc
     3001 gctaccgcac gaaggacttg tcacggctca tgccgggcac ggcatacccg acgatgcggc
     3061 ggatggcgaa ggtgaccggg cggaccgacg acatgatgat catccgcggg gtcaacgtct
     3121 tcccgaccca gatcgaggag atcatcctgc gcgagccggg gttggcgccc tacttccagt
     3181 gcgtgctgac ccagccgggg aacatggtcg agttgaccgt cgtggtcgag acgctggtgc
     3241 atgagggcga ggagcgggcc aactcgattg ccgccgacat ttcgcacgag atcaaggctc
     3301 ggatcggcac gtcggccaag gtcgagatcc gcgacccggg tggcatcgag cggtccgtcg
     3361 gcaaggccaa gcgcatcgtc gacctccgcc ccaagggcta agcactgtcc cctgccctca
     3421 cacgggagag cacgagccac tgcgctgctt cgtgtcgtcg atcagcgtgc gctcgcgatg
     3481 tcgagcgcca ggcggaccgg cccagccaca cccaacacct gggcgc
//
LOCUS       DAOMZY010000358         3517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_25692_length_3517_cov_6.216349, whole genome shotgun sequence.
ACCESSION   DAOMZY010000358 DAOMZY010000000
VERSION     DAOMZY010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25692_length_3517_cov_6.216349"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PLK64_15235"
     CDS             complement(<1..320)
                     /locus_tag="PLK64_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine pyrophosphorylase"
                     /protein_id="HPV81147.1"
                     /translation="MSTLSPGVAALLAKGVQMPLPETVHVGPEVDPDRVSGRGVTIHP
                     GCRVSGSETVVSAGVTLGAEGPVTAIDVRLGPGASIAAGYAAKTVLLEGASLGSGAHA
                     REGTL"
     gene            457..3351
                     /gene="topA"
                     /locus_tag="PLK64_15240"
     CDS             457..3351
                     /gene="topA"
                     /locus_tag="PLK64_15240"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009883364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPV81148.1"
                     /translation="MAAGKGRKLVIVESPAKAKKIAEYLGGDFEVEASVGHIRDLPNP
                     RDLPPDLKKGPFGKFAVDVDNGFEPYYVVDADKRKKVADLKRLVKESSELYLATDEDR
                     EGEAIAWHLLEALQPRIPVKRMVFHEITKEAIQRAVIDTRELDTRLVDAQETRRILDR
                     LYGYEVSPVLWRKVRQGLSAGRVQSVATRMVVERERERMAFVAASYWDVVAEVTPRAS
                     REPFTARLTGVDGRKVATGRDFGDDGRLKSAAVHLDQAAAHAIAGRLREADVTVTDVA
                     EKPYTRRPAAPFTTSTLQQEASRKLRMSSKHAMRVAQRLYENGYITYMRTDSVTLSGA
                     AVSAARRQARDLYGADYVPGSPRVYTAKVKNAQEAHEAIRPAGDHFRTPAQVAGELRG
                     EDFALYELIWKRTIASQMTDARGSTATVKLGASLRLPAAGVVELSASGTVITFRGFLA
                     AYEEGVDEDRLARAREDEAERRLPQLAVGVPMEVLDAQADGHETTPPPRFTEATLVKA
                     MEEKGIGRPSTYASTVGTIQDRGYVRTRGSALIPTWLAFAVTRLLEEHFTELVDYDFT
                     ASMEEDLDRIANGDEHRAEWLRRFYFGDEASSGQGLRELVEDLGEIDAREISTIHLGD
                     GMVVRVGRYGPYVEELPVEGSTPQGVTEIDPATPAATPRRATITDDIAPDEMTPAKAR
                     ELLDAAGDDGRVLGNDPLTGHEIVAKAGRYGPYVTEVIPDPLEAGAGTKHAKKNAKGA
                     KDVKPRTASLFKDMELATIELDVALKLLSLPRVVGLDAEGVEITAQNGRYGPYLKRGA
                     DSRSLVTEAQLFDVTLEEALAIYAQPKTRGGRTAAAPLREFGPDPVSGRPIVLKDGRF
                     GPYVTDGETNATLRKADDPATMPTERAHELIAEKRARGPVTRKRAAPARKATTKTAAK
                     ATTAKTTTAKTTARKTAATKTTAAKTAARKTAATKTTARVAKSTAPRVAD"
BASE COUNT          607 a         1267 c         1170 g          473 t
ORIGIN      
        1 agcgtcccct cgcgggcatg cgctcccgac ccgagcgagg cgccttccag gagaacggtt
       61 ttggcggcat acccggcggc gatcgacgcc ccgggtccga gccgcacgtc gatcgcggtg
      121 accgggccct ccgccccgag cgtgacgccg gccgagacga ccgtctcgga gcctgacacc
      181 cgacacccgg gatggatggt caccccgcgg ccactcacgc ggtcggggtc gacctccggc
      241 ccgacgtgaa cggtctccgg caggggcatc tggacgccct tggcgagcag ggcggcgacc
      301 cccggcgaca gcgtgctcat gaccagccag tatgccgccc gccgaggggg ccgttggaat
      361 ccccccagcc gggtccgcta cggtggcgcg ggcactgacg tccgagcagc gacgaatccg
      421 cggcacgtgg tgctcaggaa cagcaagggg tatgccgtgg cagcagggaa gggccgcaag
      481 ctcgtcatcg tcgagtcgcc ggccaaggcc aagaagatcg ccgagtacct cggtggtgac
      541 ttcgaggtcg aagcatccgt cggtcacatc cgtgatctgc ccaacccgag agaccttccg
      601 ccggacctca agaaggggcc gttcggcaag ttcgccgtcg atgtcgacaa cgggttcgag
      661 ccgtactacg tcgtggacgc ggacaagcgc aagaaggtcg ccgacctcaa acgcctggtc
      721 aaagagtcct ccgagctcta cctcgccacc gatgaggacc gcgaaggcga agcgatcgcc
      781 tggcacctgt tggaggcgct gcagccgcgg atccccgtca agcgcatggt gttccacgag
      841 atcaccaagg aagccatcca gcgcgccgtc atcgacaccc gggagctgga cacccggctg
      901 gtcgatgcgc aggagacccg ccgcatcctc gaccgcctct acggctacga ggtctcgccg
      961 gtgttgtggc gcaaggtccg ccagggtctg tcggccggtc gtgtgcagtc ggtcgcgacg
     1021 cggatggtcg tcgagcgcga acgcgagcgg atggcgttcg tcgccgcgtc ctactgggat
     1081 gtcgtggccg aggtcacccc gagggccagc cgcgagccgt tcactgcccg cctcaccggc
     1141 gtcgacggcc ggaaggtggc caccggccgt gacttcggcg acgacggccg gctcaagagt
     1201 gccgcagtcc atctcgacca ggccgccgcc cacgcgatcg ccggccggtt gcgcgaagcc
     1261 gacgtcaccg tcaccgacgt cgccgagaag ccctacaccc gtcgcccggc cgcgccgttc
     1321 accacctcga cgctgcagca ggaggcctcc cgcaagctgc ggatgtcgtc caagcacgcg
     1381 atgcgcgtcg cccaacggct ctatgagaac ggctacatca cctatatgcg gaccgactcg
     1441 gtcaccctgt cgggtgccgc cgtctcggcc gcgcgacgcc aggcccgcga cctctacggc
     1501 gccgactatg tgccgggctc gcctcgcgtc tacacggcga aggtgaagaa cgcccaggag
     1561 gcccacgagg ccatccgccc cgccggtgac cacttccgca ccccggcgca ggtcgcagga
     1621 gagttgcgcg gcgaggactt cgcgctctac gagttgatct ggaagcgcac gatcgcctcc
     1681 cagatgaccg acgcgcgcgg atccaccgcg accgtcaaac tcggcgcctc cttgaggctg
     1741 cctgccgccg gtgtcgtcga actgtcggcg agcgggacgg tcatcacctt ccgtggcttc
     1801 ctcgcggcat acgaggaagg cgtcgacgag gaccgcctgg cccgcgcccg cgaggacgag
     1861 gccgagcgcc gcctcccgca gttggccgtg ggcgtgccga tggaggtgct cgacgcgcag
     1921 gcggacggtc acgagaccac gccgccgccg cgtttcaccg aggccaccct cgtcaaagcc
     1981 atggaggaga aggggattgg tcgaccgagc acctacgcct ccacggtcgg caccatccag
     2041 gaccgcggct atgtgcgcac ccgcggctcg gccctcatcc ccacgtggtt ggcgtttgcg
     2101 gtgacccggc tgctcgaaga gcacttcacc gagttggtgg actacgactt caccgcctcg
     2161 atggaggagg acctcgaccg catcgccaac ggtgacgagc accgggcgga atggctgcgg
     2221 cggttctact tcggtgacga agcctcgtcg ggccagggcc tccgcgagct cgtcgaagac
     2281 ctcggcgaga tcgatgcccg cgagatttct acgatccacc tcggcgacgg gatggtcgtg
     2341 cgggtcgggc gctacggccc gtatgtcgag gagcttcccg tcgagggctc gacgccccag
     2401 ggtgtgaccg agatagatcc ggcgacccct gcggccacgc ctcgacgagc gacgatcacc
     2461 gacgacatcg cccccgacga gatgacgccg gccaaggccc gcgaactgct cgacgccgcc
     2521 ggcgacgacg gccgggtgct cgggaacgac ccgctaaccg gccacgagat cgtcgccaag
     2581 gcgggccgct acggccccta cgtcaccgag gtcatccccg acccgcttga ggccggcgcc
     2641 gggaccaagc acgccaagaa gaacgccaag ggcgccaagg acgtcaagcc gcggacggcg
     2701 tcgctgttca aagacatgga gctcgccacg atcgagctgg acgtcgccct caagctgctc
     2761 tccttgcctc gcgtcgtcgg tctcgacgcc gaaggggtcg agatcaccgc gcagaacggc
     2821 cgctacggcc cctatctcaa gcgcggcgcc gactcccggt ccttggtgac cgaggcgcaa
     2881 ctcttcgacg tcaccctcga ggaggcgctg gcgatctacg cccagcccaa gacgcgggga
     2941 ggccgcactg ccgccgcgcc gttgcgcgag ttcggtcccg accccgtctc cggccgtccg
     3001 atcgtcctca aggacggtcg cttcgggcct tacgtcaccg atggcgagac caacgccacg
     3061 ctgcgcaagg ccgacgatcc ggcgacgatg cccaccgagc gcgcgcacga gctcatcgcc
     3121 gagaagcggg cccgtggtcc ggtcacccgc aagcgggccg ctccggcgcg caaggcgacg
     3181 accaagaccg ccgccaaggc gacgacagcc aagacgacga cagccaagac gaccgcgcgc
     3241 aagaccgcgg cgaccaagac gaccgcggcc aagacggcag cgcgcaagac cgcggccacg
     3301 aagacgaccg ctcgggtcgc caagtccacg gcacctcgcg tcgcagactg aggtcgtccg
     3361 ggagtatgcc gtgcggcacc tcctgctcga atctcgtttc gctgcaacga aatttgtcgc
     3421 cggtcggagc catttgccga acgttaatcc acggttcaac gaccagggct acccgcgtgt
     3481 gggttcgcga gtaacctgag gaggttggcc agagtcg
//
LOCUS       DAOMZY010000359         3503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_25839_length_3503_cov_5.118619, whole genome shotgun sequence.
ACCESSION   DAOMZY010000359 DAOMZY010000000
VERSION     DAOMZY010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25839_length_3503_cov_5.118619"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PLK64_15245"
     CDS             complement(<1..637)
                     /locus_tag="PLK64_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81149.1"
                     /translation="MTLTDVGLLSQPVLAGLAFIAALAVNDSRRSAIAGTLNALLGLA
                     TAATGVGALLGEPGSLTFATGIPGIAHLGQLALAPDRLGGIFLVVVGVVGALSAWFGV
                     GYAHGATATRAAWAAYPIFLWGMSIVPAAGDAVTFLLGWELMALGSTVLLHSEHATRP
                     QVRSAVLWYAAMTHLSFVLLLAGFAVLIASANGTTWEALAASPPTGVSASLA"
     gene            complement(634..1122)
                     /locus_tag="PLK64_15250"
     CDS             complement(634..1122)
                     /locus_tag="PLK64_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HPV81150.1"
                     /translation="MSALDIAKLLRRVGRITETDTAASPSDYTPPAALAGSVQVRHVD
                     CGSCNGCEIELAGAFGPVYDAERYGARLVASPRHADVLAVTGAPTRNLAEPLRRSVEA
                     APRPRVVLAIGDCAAGEGAYAQGYAVVGGVADIVPVDLVVRGCPPPPDAIVAALRQVT
                     GK"
     gene            complement(1119..1484)
                     /locus_tag="PLK64_15255"
     CDS             complement(1119..1484)
                     /locus_tag="PLK64_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPV81151.1"
                     /translation="MHVPVPLYQAKAELFRTLGHPARIRVLELLSERDYAVHELLAAI
                     ELEPSSLSQQLAILRRTGLVRQRRVGGEVVYSIVVPATKDLLSAGRNVLRAVLADQDQ
                     LADLLDPPVLKAPRRGGRG"
     gene            1904..2884
                     /locus_tag="PLK64_15260"
     CDS             1904..2884
                     /locus_tag="PLK64_15260"
                     /inference="COORDINATES: protein motif:HMM:NF022586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HPV81152.1"
                     /translation="MVSETVAHNGLPGPLPGSPPRRAGGVRRTTSLDLTRPLGPDGPI
                     EVNGRARDVRTADAGAMTVLDKALLSLTVTDGIVTRLRSFPERAAAPRLVGRQALVGW
                     RTGLWRELHDDVESGSALHLLLDDLPGGMVVGGFTRRRALAAAGEPVPQPGRRLDVCA
                     GWAADSRAAQIMADTGTPPPPVTPEAPDLVRDDDPNGWHTLAALPPWAMSRRRRVDAH
                     LDGAQIEVDAMFRDSFIDIEGRERVLHEYAVHATLEANEGRVLAVQPAPRVLPHLECP
                     VAGASTQRLIGMPCADLRDLVSSTLFGPSTCTHLNDLMRSIADVPALLTR"
     gene            2962..>3503
                     /locus_tag="PLK64_15265"
     CDS             2962..>3503
                     /locus_tag="PLK64_15265"
                     /inference="COORDINATES: protein motif:HMM:NF015658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HPV81153.1"
                     /translation="MRVIRGALLAALLGVVSGCGATAGGSPPTSGSATGAGLSSTATS
                     TYSSRGADILFAQMTLLHHEQTVTLADLATTKAGIRSEVRELARDVRAIEEPDIAVLR
                     AWLLEWNAPERLNVHSGHPMKGILLDVEMAGMEALDGAAFESAWLVRMIAHQDGAAEF
                     AGSVVRRTGDDRVRTMARRV"
BASE COUNT          507 a         1185 c         1315 g          496 t
ORIGIN      
        1 aggccagcga agcgctcacc ccggtcggcg gcgaggcggc gagggcctcc caggtcgtgc
       61 cgttggccga cgcgatgagg accgcgaagc cggccaggag caggacgaaa ctcaggtgcg
      121 tcatcgcggc ataccaaagg acggcggagc gcacctgcgg ccgggtcgcg tgctcggagt
      181 gcagcaggac cgtcgacccg agcgccatca actcccaccc cagcaggaag gtcaccgcgt
      241 cgccggccgc gggcacgatc gacatgcccc agaggaagat cgggtatgcc gcccacgcgg
      301 cacgcgtggc tgtggcgccg tgggcgtagc cgactccgaa ccaggcgctg agggccccga
      361 cgaccccgac gacgacgagg aagatacccc cgagtcggtc gggggcaagg gcgagttggc
      421 cgaggtgggc gatcccgggg atgccggtgg cgaaggtcag cgagccgggc tcaccgagca
      481 gggctccgac gccggtggcg gccgtggcca ggccgaggag cgcgttgaga gtgccggcga
      541 tggccgagcg gcgggagtcg ttgacggcca gcgcagcgat gaaggccagg cctgccagca
      601 ccggctggct gagcagcccg acgtcggtga gggtcatttg ccagtgacct gtcgcagtgc
      661 ggcgacgatg gcgtcaggag gcggcgggca gccgcgcacg acgaggtcga cggggacgat
      721 gtcggcaacc ccgccgacga cggcataccc ctgggcgtag gcgccctccc cggcggcgca
      781 gtcgccgatg gcgaggacga ccctcgggcg gggtgcggct tcgacgctgc ggcgcaacgg
      841 ctcggcgagg ttgcgggtcg gtgcgccagt gacggccagg acgtcggcgt gccggggcga
      901 ggcgaccagc cgggcgccgt agcgttcggc gtcgtagacc gggccaaagg cgcccgccag
      961 ctcgatctcg cacccattgc atgagccgca atcgacgtgc ctgacctgga cgctcccggc
     1021 gagggccgcg ggtggcgtgt agtcggatgg tgatgcggcg gtgtcggttt cggtgatccg
     1081 accgacgcga cgcaggagtt tggcgatgtc gagcgcgctc atccgcggcc accccgccga
     1141 ggggccttga gcacgggagg atcgagcagg tcggccagct ggtcctggtc ggcgaggacc
     1201 gcgcgcagga cgtttcggcc ggcgctcagc agatctttgg tggccgggac gacgatcgaa
     1261 tagaccacct cgccccccac tcgacgctgg cgcacgaggc cggtgcgccg gaggatggcg
     1321 agttgctgcg agaggctgct cggctcgagc tcgatggcgg ccaggagttc gtggacggcg
     1381 tagtcgcgct cggagagcag ctccagcacc cggatccgcg cggggtggcc cagggtgcgg
     1441 aagagctcgg ccttggcctg atagagcggg accggcacgt gcatcacccc ttcacctgtt
     1501 catgtgacca attgcgcaac tattcaagtc tggcacatga ggaagggtcg gccaagtggg
     1561 ccggtcagcg ctgaggcgtt ggggtcctgg actgggtctc gcggcaggtg cagcgctctg
     1621 acacggggaa ctgggccatg acggcgtcga cgtgcatgcc gcacccccgc caggtgacct
     1681 tgttgcaggt ggagcagcga gccggactgc acatcgtgga gcctccttgt cgtcgtgtgg
     1741 ggctatggtg acatataccc ctgggggtat tcaagtcgag ccccggcgag ttgcgtcatg
     1801 ccagacgcgc aacctatctc gcgttcggca tgacgcacct gccgttgtgt cggcggtgga
     1861 caggccgtgg gcgggcggtg ggcgggcggc ataccctcgg gggatggtga gcgagacggt
     1921 ggcgcacaac gggcttcccg gccccctgcc cggctcgccg ccgcgtcgag cggggggcgt
     1981 gcggcgcacg accagcctcg acctgactcg gccactcggc cccgacgggc ccatcgaggt
     2041 caacggccgg gctcgcgacg tgcgcaccgc cgacgcgggc gccatgaccg tgctcgacaa
     2101 ggccctgctg tcgctgacgg tcaccgatgg catcgtcact cggctgcgga gttttccgga
     2161 gcgggccgcc gccccgcgcc tcgtcggtcg ccaggcgctc gtgggctggc ggaccggtct
     2221 gtggcgcgag ttgcacgacg acgtcgagtc ggggtcggcc ttgcacctgc tcctcgacga
     2281 ccttcccggc ggcatggtcg tcggcgggtt cacccggcgg cgggcgctcg ccgcggcggg
     2341 cgagccagtg ccccaacccg ggcgccgcct ggacgtctgc gccgggtggg cggccgattc
     2401 gcgggccgcc cagatcatgg ccgacaccgg caccccgcct ccgccggtca cccccgaggc
     2461 tcccgacctg gtccgtgacg acgaccccaa cggctggcac acgctggccg cgctgccgcc
     2521 gtgggcgatg tcgcgtcgcc gccgggtcga tgcccacctc gacggggccc agatcgaggt
     2581 cgacgcgatg ttccgcgact cgttcatcga catcgagggg cgcgagcggg tcctgcacga
     2641 atacgcggtc cacgccacct tggaggccaa cgagggtcgc gtcctcgccg tccaaccggc
     2701 accgcgggtg ctcccacacc tggaatgtcc cgtcgccgga gcatccaccc agcgcctcat
     2761 cggtatgccg tgtgccgacc ttcgcgacct cgtctccagc acgctgttcg ggccgtcgac
     2821 ctgcacccat ctcaacgacc tcatgcgctc gatcgccgac gtccctgccc tgctgacccg
     2881 ttgagttcct gcgcccgagg ggcgtcgggc gcgtggggca atcgccgtcg tgcggccggg
     2941 caaataggac agactggccg catgcgggtc attcggggcg cccttctggc ggcccttctc
     3001 ggggtggtga gcgggtgtgg ggcgacagcg ggcggtagcc ccccgacctc cgggtccgcg
     3061 acaggtgccg ggctgtcgtc cacggccacc tcgacgtatt ccagcagggg cgctgacatc
     3121 ctgttcgccc agatgacgct gctgcatcac gagcagaccg tcacccttgc cgacctcgcg
     3181 accaccaagg ccggcatccg ctctgaggtg cgcgagttgg cgcgggacgt gcgggcgatc
     3241 gaggagccag acatcgccgt cctgcgcgcc tggttgctcg aatggaacgc ccccgagcga
     3301 ctcaacgtgc acagcgggca cccgatgaag gggatccttc tggacgtcga gatggccggg
     3361 atggaagcct tggacggcgc cgccttcgag agcgcctggc tggtgcggat gatcgctcac
     3421 caggacggtg ctgccgagtt cgccgggtcg gtggtgcggc ggaccggcga cgaccgggtc
     3481 cggacgatgg cccgcagggt gat
//
LOCUS       DAOMZY010000360         3502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_25855_length_3502_cov_4.389904, whole genome shotgun sequence.
ACCESSION   DAOMZY010000360 DAOMZY010000000
VERSION     DAOMZY010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25855_length_3502_cov_4.389904"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PLK64_15270"
     CDS             complement(<1..181)
                     /locus_tag="PLK64_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPV81154.1"
                     /translation="MRVVIAEDSTLLREGLVRLLALDGIETVAAVDTAPALIDAVDTL
                     APDLAVIDVRMPPTFS"
     gene            complement(169..1446)
                     /locus_tag="PLK64_15275"
     CDS             complement(169..1446)
                     /locus_tag="PLK64_15275"
                     /inference="COORDINATES: protein motif:HMM:NF025168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPV81155.1"
                     /translation="MATTRHTEGQQPAGAIGLRYRGIRPGYAVLHVATGGLIGIFAFT
                     ALITLASLTVGLLPTVIGAVVCAFLLVGFNTGLAALHRSRMRAFLGLHIVAPTPPNRH
                     GWAGLLQVLTRAAFWKSLTYNAIVGLVLFILATVLLAFLAIGVILATGTVTPLPVPMP
                     PLWPDQGHSPVATVTGFALAVGAILLVPYVARAVAYGDGLVAVALLGRTSTDELAEQV
                     VHLAQTRTDTVDAADAERRRIERDLHDGTQQRLTALAMNLGVARATLPDLSPPAQAAL
                     EEAHAEAKAALVELRGIVRGLHPAVLDDRGLDAALSGLAARSPVPVSLDVHVPGRLSR
                     PVEAVAYFVVSEALTNVVKHSGASRAQVVARVDVGTLRLRIVDDGAGGADDSRGTGLT
                     GLRQRVASVDGTFTVESPLGGPTVLEAWIPCGS"
     gene            complement(1537..2907)
                     /locus_tag="PLK64_15280"
     CDS             complement(1537..2907)
                     /locus_tag="PLK64_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPV81156.1"
                     /translation="MSELDTQGSSPEPWYSAAPTTTLPPTTTLPPTTTLPQTQSALPP
                     PPPGALYPPAGHTAYPPIPSPAPTPTPTFMAPKPPREKRRVGDLVVVALLSAGLASGG
                     TYAVARATAGTTATTTAASQASNNPPAAAPVIQANGTAPDWTATAKAVSPSVVAIKVA
                     GASGEGEGSGVVIDKAGHVLTNNHVATGAGAGATISVTLDDGRTYAATIAGTDPSTDL
                     AVLTLTNPPSDLTPIVVGDSDALVVGQAVMAVGNPLGLAGTVTTGIVSALNRPVTTGS
                     ADATRGQSGSDTVVTNAIQTSAAINPGNSGGALVTSDGRLVGINSSIASLGSSNSQSG
                     NIGIGFAIPSNEAKTIADQLIATGSAQHPYLGVSSTDGTVADGSSQRTAAVIRTVSPG
                     TPAASAGLQTGDAVVAMNGDRIDSSLSLVAAIREQAVGKEVTLTIVRNGSREDVKVTL
                     TSRPAN"
     gene            3083..>3502
                     /locus_tag="PLK64_15285"
     CDS             3083..>3502
                     /locus_tag="PLK64_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81157.1"
                     /translation="MQTQGVDVGALLRRVAPMWLAWVLLAVPALWMVVVYLSLADAQF
                     PTDVQLYDDAGNPISQEGPGNQVGAFGRIYLGLLLGLGNATLTFELPLLGLIGAVGVC
                     VLWAGQRRQVGFRSAAALGAAISLALTAVGPWLILARE"
BASE COUNT          479 a         1116 c         1368 g          539 t
ORIGIN      
        1 cggagaaggt cggtggcatc cgcacgtcga tgacggcgag gtcgggggcc agcgtgtcga
       61 cggcgtcgat gagcgccggc gcagtgtcga cggccgccac ggtctctatg ccgtcgaggg
      121 ccagcagccg gacgagcccc tcgcgcagca gggtggagtc ctcggcgatc acgacccgca
      181 tgggatccac gcctccagca cggtggggcc tccgaggggg ctttcgacgg tgaaggtgcc
      241 gtcgaccgag gccacccgtt gccgaaggcc ggtgagcccg gttccgcggg agtcgtccgc
      301 tccgccggca ccgtcgtcga cgatccgcaa ccgcagcgtt ccgacatcga ccctcgcgac
      361 gacctgggcc cggctggctc cagagtgctt gaccacgttg gtcagcgcct cagagaccac
      421 gaaatacgcg accgcctcga ccgggcggga caaccggccg ggcacgtgga catcaagcga
      481 gacggggacg ggggaccggg ccgccagccc ggagagggca gcgtcgagtc ctcggtcgtc
      541 gagcacggcc gggtgcaggc cccgcacgat gccccgcaac tcgacgaggg cggccttggc
      601 ctcggcatgg gcctcctcca acgcggcctg ggccggtggg gacaggtccg gaagggtcgc
      661 ccgagccacc ccgaggttca tcgccagagc cgtcagccgc tgttgggtcc cgtcatgcag
      721 gtcccgctcg atgcgccgtc gctcagcatc ggccgcatcg acggtgtcgg tgcgggtctg
      781 ggccaggtgg acgacttgct cggccagctc gtcggtcgag gtgcggccaa gcagggcgac
      841 ggcgacgagt ccatcgccat aagccacggc gcgagccacg tacggtacga ggaggatggc
      901 gcccacggcc agggcgaagc cggtgactgt ggcgaccggg ctatgtcctt ggtcgggcca
      961 gagcggaggc atcgggaccg gcaacggggt caccgtgccg gtcgccagga tcacaccgat
     1021 tgccaggaag gccagcaaga cggtcgccag gatgaacagc accagcccga cgatggcgtt
     1081 gtaggtcaac gacttccaga acgctgcccg cgtcaggacc tggagcaaac cggcccaacc
     1141 gtggcgattc ggcggtgtcg gcgcgacgat gtgcagcccg aggaacgccc tcatccggga
     1201 gcggtggagg gccgccaggc cggtgttgaa ccccaccagg aggaaggcgc acaccacggc
     1261 gccgatgacc gtgggcagca gcccgacggt caacgacgcc aacgtgatga gggccgtgaa
     1321 ggcgaagatg ccgatcaggc cgcccgtggc gacgtgcagc acggcatacc cgggccggat
     1381 gccgcggtaa cgcaagccga tcgcgcccgc gggctgctgc ccttcggtat gccgtgtggt
     1441 tgccatggcc ccacgctacg ggccggctgg gtggctgtct ctgcagtagg ggaccgtttg
     1501 tggggtggtg caggcaccac cccacaaacg gtcaggtcag ttcgcgggcc gcgacgtcag
     1561 ggtgaccttg acgtcctcac gggagccgtt tcggacgatt gtcagggtga cctccttgcc
     1621 gaccgcctgc tcgcggatgg cggcgaccag gctgagcgac gagtcgatcc ggtcgccgtt
     1681 catcgcgacg acggcgtcgc cggtctgcag tccggcggac gcggcggggg tcccggggct
     1741 caccgtgcgg atgactgccg cagtgcgctg ggacgaaccg tcagccaccg tcccgtcggt
     1801 ggaggacaca ccgaggtagg ggtgctgcgc cgaaccggtg gcgatgagtt ggtcggcgat
     1861 cgtcttggcc tcgttggagg ggatggcgaa gccgatgccg atgttgccgc tctggctgtt
     1921 ggaggaaccg agcgatgcga tggacgagtt gatcccgacc agtcggccgt cggaggtgac
     1981 gagggcgccg ccggagttgc cggggttgat ggcggccgag gtctggatgg cgttggtgac
     2041 cacggtgtcc gagccgctct ggccacgcgt tgcgtcggcc gaacccgtcg tcaccgggcg
     2101 gttgagcgcc gagacgatgc cggtcgtcac cgtgcccgcc agccccagcg ggttgcccac
     2161 cgccatgacc gcctggccga cgacgagggc atcgctgtcg ccgacaacga tcggcgtgag
     2221 gtcgctcggg gggttggtga gggtgagcac cgcgaggtcg gtggaggggt cggtcccggc
     2281 gatcgtcgcg gcatacgtgc gaccgtcgtc gagggtgacc gagatggtgg cgccggcgcc
     2341 ggctccggtg gccacgtggt tgttcgtgag gacgtgaccg gccttgtcga tgacgacgcc
     2401 ggatccctcg ccctcacccg aggcgccggc caccttgatg gcgacgacgc tcggtgagac
     2461 ggccttcgcg gtggcggtcc agtcgggggc ggtgccgttg gcctggatga ccggggcggc
     2521 cgccgggggg ttgttcgagg cctgcgaggc cgcggtggtc gtcgccgtgg tgcccgcggt
     2581 ggcgcgggcc acggcatagg tgccgccgga ggcaagcccc gcggacagca gggcgacgac
     2641 gacgaggtct ccgacgcggc gcttctcgcg gggcggcttc ggtgccatga acgtgggagt
     2701 gggcgtgggc gcggggctcg ggatcggcgg gtatgccgtg tgccccgcgg gtgggtacag
     2761 cgcgccgggg ggcggcggcg ggagcgccga ctgcgtctga ggcagcgtcg tggtgggagg
     2821 cagcgtggtc gtgggaggca gcgtggtcgt gggcgctgcc gagtaccacg gctcgggcga
     2881 cgagccctgg gtgtcgagtt cgctcactgg agttctcctt ggcgcggatt ccgccggtct
     2941 ggcggctccc tttactgcac cttcggcggt tgggcgttgg ctgagggcaa cctgtgacca
     3001 tcctgtgggt gtgccggcat ggctgggatc ggtggccatc accgcccggg cccggccgcg
     3061 tggatgccgt accgtgctgg ccatgcagac tcagggggtc gatgtcggcg cgttgctgcg
     3121 cagggtggcg ccgatgtggt tggcgtgggt gctgttggcc gtgccggctc tgtggatggt
     3181 cgtcgtctat ctgtcccttg cggacgcgca gttccccacg gacgtccagc tctacgacga
     3241 cgccggcaac cccatcagtc aggaggggcc cggcaatcag gttggggcct tcggccgcat
     3301 ctacctcggc ctgctcctcg ggttgggaaa cgccacgctg acgttcgaac tgcccctcct
     3361 cggcttgatc ggcgcggtcg gtgtctgcgt gctgtgggcc ggtcagcgca ggcaggttgg
     3421 gtttcgctcg gcggcggcgc tgggggcggc catctcgctg gccctcacgg cggtcggccc
     3481 ttggctcatc ttggcgcggg ag
//
LOCUS       DAOMZY010000361         3489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_26003_length_3489_cov_8.789458, whole genome shotgun sequence.
ACCESSION   DAOMZY010000361 DAOMZY010000000
VERSION     DAOMZY010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26003_length_3489_cov_8.789458"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..110
                     /locus_tag="PLK64_15290"
     CDS             <1..110
                     /locus_tag="PLK64_15290"
                     /inference="COORDINATES: protein motif:HMM:NF020906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1990 family protein"
                     /protein_id="HPV81158.1"
                     /translation="AYSRPASTLAKFGGPISRAVQRFMTQRYLEALDRL"
     gene            complement(154..564)
                     /locus_tag="PLK64_15295"
     CDS             complement(154..564)
                     /locus_tag="PLK64_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HPV81159.1"
                     /translation="MSTSWLIDKSALVRLGWSPDAEEWANRVERGLVAISSVTLLEAG
                     FSARSAVELARAMGSPPLAAMPVEYLTPAAEDRAVEVQRLLAERGQHRAPSIPDLLIA
                     AAAELNQRTVLHLDKDFDLIAELTAQPVERLRIE"
     gene            complement(561..782)
                     /locus_tag="PLK64_15300"
     CDS             complement(561..782)
                     /locus_tag="PLK64_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HPV81160.1"
                     /translation="MPDVLIRNFPAEDLNLLDEQAARLGISRTEYLRRHLHREARRSA
                     GSVTAGDLRAVAELLPDLADDAIMSDAWS"
     gene            complement(1660..1965)
                     /locus_tag="PLK64_15305"
     CDS             complement(1660..1965)
                     /locus_tag="PLK64_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81161.1"
                     /translation="MICVQFIFKPGTYDEDFHRLDGQIDDYARTLPGFVRTETWRNPE
                     QGLVNAIYYFTDQKSVAYLARFPVHREAKGQVHRWYEGYRIVVTEVKATYGDGRLPE"
     gene            complement(1962..2711)
                     /locus_tag="PLK64_15310"
     CDS             complement(1962..2711)
                     /locus_tag="PLK64_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HPV81162.1"
                     /translation="MAELDSSAPLAHAHVSEEVFDLRPDYRALLVTAEGIDPRSGDAA
                     SEELLRQAEAHATALLAAGSVEQLPHVAAWREAYRGFGAKPQRTRNSLEALTRRAPSG
                     LPRVNALTDTYNAISVLHQVPIGGEDLVRYAGSPRLVRATGAEPFDTTADGQPVIENP
                     DVGEVVWCDDAGVTCRRWNWRQGRRTALRPDTTNALFIIDALVPVTDADLHAVSAALS
                     NHLRGLGPAVRIRSRLIDRAASSLADREEPS"
     gene            complement(2704..3309)
                     /locus_tag="PLK64_15315"
     CDS             complement(2704..3309)
                     /locus_tag="PLK64_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HPV81163.1"
                     /translation="MDEQAAALASVIGSRVRAERHGKGWTLDHLAGSAGVSRRAVVNV
                     EQGVANPSVGTLLRISDALGVGLPALVSPAEPLSVPVTRAGDGAVLWSGEHGGRGVLV
                     AGTEPPDVAELWDWTMSAGERHDSVAHAAGTQELLQVLVGTLTLVVADQRKTLSAGDA
                     VAFPGDVSHGYANESGATVQFTLAVFEPGVGSAPKADRSHG"
BASE COUNT          568 a         1174 c         1161 g          586 t
ORIGIN      
        1 ccgcgtactc gcggccggcc tcaacactgg ccaagttcgg tggcccgatc agccgggccg
       61 tgcaacgctt catgacccag cgttacctcg aggcattgga tcgcctgtag ctaacatcct
      121 gaactgccgc gatccgtatg agtgtggctg gttctattcg atgcggaggc gttcgacggg
      181 ttgggcggtg agttcggcga tgaggtcgaa atctttgtcc aggtgcagga cggtgcgctg
      241 gttcagttct gcggcggcgg cgatcagcag gtcggggatg gacggggcgc gatgctggcc
      301 gcgttctgcc aacaggcgtt gcacctcgac tgctcggtcc tcggccgcgg gagtcaggta
      361 ctccacgggc atcgctgcca acggtggtga ccccatcgct cgggccagct ccacggctga
      421 acgggcggag aagccggcct cgagcagggt gacgctgctg atggcgacga gtcctcgctc
      481 gacccggtta gcccactcct cagcgtcggg gctccaaccc agccgaacga gggcggactt
      541 gtcgatcagc caggacgtgc tcatgaccag gcatcggaca tgatcgcgtc gtcagcgagg
      601 tctggcagga gctctgccac ggcacgaagg tcacccgctg tcaccgagcc tgccgagcgg
      661 cgggcctcac ggtgaaggtg ccggcgcagg tactcggtgc gagagatgcc cagtcgggca
      721 gcctgctcat cgagcaggtt gaggtcttct gccgggaagt tgcgaatcag gacgtcgggc
      781 atggtgcacc tcctagatat ctaacgatat cactccctcg agacgcaccc acatgccgca
      841 tatctgaagc attgaggcct gcggtcactt cggaggaacg tccttctcgg ctgggctcat
      901 ctcgacggtc tgcccggtcg atcggtcaac aacggtgcgc gtgccaagcg gcgcgttcag
      961 gaccactgtg acgccatccg cgcagtcgcc ggcgttccga ccgaaccaac gcccgctcgt
     1021 gacttggact cgcacctcgg tatcggactc catcacttcg gcagtcgggt tctcgttgca
     1081 tgacgcgacg ttcagctcca agcgcgtgga cgcggggtcg ccgtaggccg cgaagaccga
     1141 tcgactccgg ccatcgcacg ccgtcgcggc caggaccacg caagccccaa cgaccatcgc
     1201 tccggccctg cggtgaagtc ccacgttccg aaggtagcga ccaacccgac aacaccgctc
     1261 ccctcacggc ctcaaacgtc cggaactgcc gcgatccggt gcgccggtgc gcagcgccac
     1321 tatcgtcggg cacccagaac aacaagtggt ttccgctact ggatcagggc agtgctgggg
     1381 cccgcgacct tcgttggcgc cacctatctg ccccacggcg gctggtggat gctcgcgtgg
     1441 atggcgctca tcgtcttggg tgggtggcta gcgttctggc cattcttcgt acccgagact
     1501 cccgccgcgg accaagcaga ctgacagtgg cgtgatccga acatgcgcgc gtcaggcacc
     1561 tccgccgagc tgacgtccgc caccggcgcg accttccgct aaatgccgcc ggtcgggccg
     1621 tgtcggtggt cggcgtccag gcgccctacg ggcgggtgct cactcgggaa ggcgcccgtc
     1681 gccgtaggtg gccttcacct cagtgacgac gatgcggtag ccctcgtacc agcggtggac
     1741 ctgccccttg gcttcacggt ggacaggaaa gcgggcgagg taggcgacgg acttctggtc
     1801 ggtgaagtag tagatcgcgt tgaccagccc ctgctcgggg ttgcgccagg tctcggtcct
     1861 gacgaacccc ggcagggtcc tggcgtagtc gtcgatctgt ccgtcgaggc ggtggaagtc
     1921 ctcgtcgtag gtgccgggct tgaagatgaa ctggacgcag atcacgacgg ttcctcgcgg
     1981 tcggcaagtg aggacgcggc acggtcgatg agccggctgc ggatgcggac ggccgggccg
     2041 aggccccgga ggtggttcga cagcgcagcc gagaccgcgt gaaggtcggc atcggtcacc
     2101 gggacgaggg catcgatgat gaacagcgcg ttcgtcgtgt cgggtcgcag ggcggtgcgt
     2161 cgtccttggc gccagttcca ccgccgacac gtcacccccg cgtcgtcaca ccagacgact
     2221 tctccgacat cggggttctc gatgacaggc tgaccgtcgg cggtggtgtc gaaaggctcg
     2281 gccccggtgg cgcgcaccag cctcgggctg ccggcatacc tgacgaggtc ctctccgccg
     2341 atggggacct ggtgcagaac ggagatggcg ttgtacgtgt cggtcagggc gttcacgcga
     2401 ggcagacccg acggggctcg ccgggtcagc gcttcgaggc tgttgcgggt gcgttgcggc
     2461 ttggcgccga acccgcggta cgcctcccgc cacgccgcga cgtgcggaag ctgctccacg
     2521 gacccggcgg cgagcagggc ggtcgcatgg gcttcggcct gccgaagcag ctcttcactc
     2581 gccgcgtcgc cggatcgagg gtcaatgccc tcggcagtca ccaggagagc ccggtagtca
     2641 gggcgcaggt cgaagacctc ctcgctgacg tgggcgtgtg caagtggtgc cgaagagtcg
     2701 agctcagcca tgcgaacggt ccgccttggg agccgagccc acgccaggct cgaagaccgc
     2761 cagggtgaac tgcaccgtgg cgccgctttc gttggcgtac ccgtgcgaga cgtctcccgg
     2821 gaacgccacc gcatcaccgg cggacaaggt cttcctctgg tcggcgacga ccaacgtcaa
     2881 cgtcccgacg aggacctgga ggagctcctg ggttcctgct gcgtgcgcca cgctgtcgtg
     2941 ccgctcgccg gcggacatcg tccaatccca cagctcggcc acgtccggcg gttccgtgcc
     3001 ggcgaccaga accccacggc caccatgctc cccgctccac agcactgccc cgtcgccggc
     3061 gcgcgtcacc ggcaccgaca acggttccgc agggctcacc agagccggca gtcccacgcc
     3121 caacgcatca ctgatgcgca gcaacgtccc gacgctgggg ttggccaccc cctgctcgac
     3181 gttcacgacg gcccgacggc tcacgcctgc ggaaccagcc agatggtcca gggtccaccc
     3241 cttgccgtgt cgctccgcgc gaacccggga cccgatcacc gatgcgagag ccgctgcctg
     3301 ttcgtccaca agtgcatcat agtgcactca tcgtgcagtt tcggccgact gctgccgacg
     3361 tgcaagaggc gtccgctgat gccgcaacca gcgagggttg taccgctgga caccaagcta
     3421 cattctggtc atagttgtga ctatgacgag catttcactg gccgacgcca aggcacacct
     3481 gtccgacgt
//
LOCUS       DAOMZY010000362         3459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_26296_length_3459_cov_8.433901, whole genome shotgun sequence.
ACCESSION   DAOMZY010000362 DAOMZY010000000
VERSION     DAOMZY010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26296_length_3459_cov_8.433901"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..703
                     /locus_tag="PLK64_15320"
     CDS             176..703
                     /locus_tag="PLK64_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV81164.1"
                     /translation="MSESIDPITVRRLKDADWPEFRRVRLAALGESPEAFSATLAEEE
                     ASDEASWLERVQRSPRFLAESGAVVGMASLGRYQGAEGESENSGELYGLWVTPALRGT
                     GVATALVNAASRQASDDGRSHVVYWVSTSNGRAVAFASGLGFRPTDDRRPMRGVGHAD
                     EEEIAMTLPLGLDRG"
     gene            848..2041
                     /locus_tag="PLK64_15325"
     CDS             848..2041
                     /locus_tag="PLK64_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HPV81165.1"
                     /translation="MPSQARVVWDPDFTKYNFGPSHPMQPVRLALTARLCEEFGIFTD
                     GDVEVLAPDVVADAWLQTVHEPYFIDAVKAASNDPEHTNEHLGIGTDDVPTFAGMHEV
                     SARIVEGTRAVCEAVWTGETAHGVNFCGGLHHAMAANASGFCVYNDIAVGIQWLLDHG
                     ADRVAYVDVDVHHGDGVESIFWNDPRVLTVSIHETGRALFPGTGWATDIGGEAALGHA
                     VNVSLPPGLSDSGWLRALHSVAMPVVRAFRPQVLVTQHGADTHFQDPLAHFALSLDAQ
                     REAMVALHRLSHEVCGGKWVALGGGGYEVIDVVPRAWTHLTAIAAHRPILPGTAVPQA
                     WRDYVESLFGRPGPARMGDLPDGEPIWWRSWDLGYDPGNEVDRAVMATRQAVFPLHGL
                     DVWFD"
     gene            complement(2100..3368)
                     /locus_tag="PLK64_15330"
     CDS             complement(2100..3368)
                     /locus_tag="PLK64_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HPV81166.1"
                     /translation="MATRRQITKAQVDRYRTGTKAEKSALLDAICQVTGWHRDHARKA
                     LRQRLAGPAPVRAPRVPTFQYDQAVIDALVTCWSVLDGPTGKRLRPGLPTLVPTMLAH
                     QELTCPPQVIDQLLGMSAATIDRRLRPYRTGLVATKGQSMTRPGSMLKSAIPMKTWHD
                     WDDTRPGFLQIDLVGHDGGDNNGAFHYTLDATDVATGWTESITVRTKGERIVSAGLEQ
                     LRLAFPFAVLGIHSDNGSEFINHHLLRWCDTRQITFSRGRSNHSNDQAHIEQKNWSEV
                     RRAVGYYRYDTTRELDLLNELWPLQSILDNLFLPQQKLATKTRNGAKVTKTYDQGATP
                     YTRLTRDHPDALHPHDHAALHARLTEINPAGLRRQISAIQATLIELARRRGKVEQRPK
                     ANATYLSRRKMTTPKRATTDESTTHNTRAS"
BASE COUNT          472 a         1057 c         1279 g          651 t
ORIGIN      
        1 caacaccgcg gcccgcgccc tctatgatcg gcacggcttc caggtggtcg ctgatgccgt
       61 gctgagcgag gatcgccggg cgagcggctg cgagctgacc atggtgcgcc gcctgcgatg
      121 agagcgcccg cggacaaccc cctgcgcagc cgtcccgccg tgaaagggtg agcccatgag
      181 cgagagcatc gatccgatca ccgtgcgccg gctgaaggac gccgactggc cggaattccg
      241 acgggtgcgt ctggccgccc tcggtgagtc gcccgaggca ttctcggcga ctctggcgga
      301 ggaggaggcc tcggacgagg cgtcctggct cgagcgggtg cagcggtcgc ctcgcttcct
      361 cgccgagtcc ggcgccgtcg tgggcatggc cagcctcggc cgctaccaag gcgcggaggg
      421 cgagtcggag aattcgggcg agctctacgg gctgtgggtc acgcccgcgt tgcgtggcac
      481 gggggtggcg acagccctgg tcaacgccgc cagccgtcag gcaagcgacg acggacggtc
      541 ccacgtcgtc tattgggtga gcacgtcgaa cggccgagcg gtcgccttcg cgtcgggctt
      601 gggcttccgg cccaccgacg accgccgtcc gatgcgtggc gttggccacg ctgacgagga
      661 ggagatcgcc atgaccttgc cgctggggtt ggaccgcggt tgaccgatcg ctcgggtctg
      721 cgtgcgtgcg cgaccacctg gaagaacggc ataccgaatc gcgttccgct gcgcggcatc
      781 ggtgcccgtg agaggtctgt gtcggccgtc gtcgtgaccg cagcggagaa ggggtagcgt
      841 cgtccttatg cctagccagg cccgggtcgt ctgggacccg gatttcacga agtacaactt
      901 cgggccctcg caccccatgc agccggtccg cctggccctg accgctcggc tctgtgagga
      961 gttcggcatc ttcaccgacg gcgacgtcga ggtgctggct cctgacgtcg tcgccgacgc
     1021 ctggctgcag acggtgcacg agccctactt catcgatgcg gtgaaggcgg cttcgaacga
     1081 ccccgagcac accaacgagc acctgggcat cggcaccgac gacgtgccga cgttcgcagg
     1141 gatgcacgag gtgagcgcgc gcatcgtcga gggcactcgg gccgtgtgcg aggccgtctg
     1201 gaccggtgag accgcccacg gcgtcaactt ctgcggcggc ctgcatcacg cgatggcggc
     1261 caacgcctcg ggcttctgtg tctacaacga catcgcggtc ggcatccagt ggctgctgga
     1321 ccatggggcc gaccgggtgg cttacgtcga cgtcgacgtc caccacggcg acggcgtcga
     1381 gtcgatcttc tggaacgacc cccgggtgct caccgtgtcg atccacgaga cggggcgggc
     1441 gctcttccct ggcaccgggt gggcgaccga catcggcggc gaggcggccc tgggccatgc
     1501 ggtcaacgtg tcgctgccgc cagggctgtc cgactccgga tggctgcgcg cgttgcactc
     1561 ggtggcgatg ccggtggttc gggccttccg cccgcaggtg ctcgtcaccc agcacggggc
     1621 ggacacgcac ttccaggacc cgttggccca cttcgccctc tcgctggatg cgcagcggga
     1681 agcgatggtc gccctgcaca gactcagcca cgaggtgtgc ggtgggaagt gggtggcgtt
     1741 gggtggcggc ggctacgagg tcatcgatgt cgtcccgcgc gcctggacgc atctgacggc
     1801 gatcgccgcg catcgcccga tccttccggg aaccgccgtg ccgcaggcct ggcgggacta
     1861 cgtcgagtcg ttgttcggtc gtccggggcc cgcgcggatg ggtgaccttc ccgatgggga
     1921 gccgatctgg tggcgctcct gggatctggg ctacgacccc ggcaacgagg tcgaccgagc
     1981 cgtgatggcg acccgacagg ccgtcttccc gctgcatggt ctggacgtct ggttcgactg
     2041 atcggcgtgt cggctggact tttcgtcaca agctgatctc gcgaccatgt tgactcatgt
     2101 caagatgccc gcgtgttgtg ggtcgttgac tcatcggtag ttgcccgctt gggggtggtc
     2161 attttccgtc tgctcaggta ggtggcgttg gctttgggtc gttgttcgac tttgccgcgg
     2221 cggcgggcta gttcgatcag ggtggcttgg atggcgctga tctgtcggcg gaggccggct
     2281 gggttgattt cggtgagtcg ggcgtgcagg gcggcgtggt cgtgggggtg cagggcgtcg
     2341 gggtggtcgc gggtgagtct ggtgtagggg gtcgcgccct ggtcgtaggt tttggtgact
     2401 ttggcgccgt tgcgggtctt ggtggcgagt ttctgttggg ggaggaacag gttgtccagg
     2461 atcgactgca ggggccacag ttcgttgagt aggtcgagtt ctcgggtggt gtcgtagcgg
     2521 tagtagccga cggctcggcg gacctcggac cagttcttct gttcgatgtg ggcttggtcg
     2581 ttgctgtggt tggagcggcc gcggctgaag gtgatctgac gggtgtcgca ccagcgcagg
     2641 aggtggtggt tgatgaactc ggacccgttg tcgctgtgga tgcccaggac cgcgaacggg
     2701 aacgccagtc gtagttgttc caggccggcg gagacgatgc gttctccctt ggtgcgcacc
     2761 gtgattgatt cggtccatcc ggtggcgacg tcggtggcgt ccagggtgta gtggaacgcg
     2821 ccgttgttgt cgccgccgtc gtggccgacc aggtcgattt gcaggaaccc ggggcgggtg
     2881 tcgtcccagt cgtgccaggt cttcatgggg atcgcggact tgagcatgct gccggggcgg
     2941 gtcatcgact ggcctttggt ggcgaccaga ccggtccgat agggacgcag ccgccggtcg
     3001 atggtggccg cggacatgcc cagcaactgg tcgatcacct gcggggggca ggtcagttcc
     3061 tgatgggcca gcatggttgg taccagtgtc ggcaggcctg ggcgtagtcg tttgccggtg
     3121 gggccgtcca gcacgctcca acaagtcacc agggcgtcga tgaccgcctg gtcgtattgg
     3181 aaggtcggca cccggggggc ccgtaccggt gccgggccgg ccaaccgttg acgtaacgcc
     3241 ttacgggcat ggtcgcggtg ccagccggtg acctggcaga tcgcgtccaa cagggccgac
     3301 ttctcggcct tcgtgccagt gcgataccga tcgacctgcg ccttggtgat ctgtcgccgt
     3361 gtcgccaacg tgagctccat ccgtcatcgt gacccacgcg ggcatccttg gacgagtcaa
     3421 cgacccccat cacgcgggca acttggatga gtcaactcg
//
LOCUS       DAOMZY010000363         3437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_26522_length_3437_cov_6.195742, whole genome shotgun sequence.
ACCESSION   DAOMZY010000363 DAOMZY010000000
VERSION     DAOMZY010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26522_length_3437_cov_6.195742"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="PLK64_15335"
     CDS             complement(<1..789)
                     /locus_tag="PLK64_15335"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HPV81167.1"
                     /translation="MDRQQEFVLRTIEERDIRFVRLWFTDVVGTLKSVAVAPAELEGA
                     FAEGIGFDGSAIEGFARVYEADMLVKPDPSTFQVLPWRGENPGTARMFCDLLTPDGQP
                     SFADPRHVLRRALSRAANLGFTFYTHPEIEFFLLHPPKGKRITPVPVDDGGYFDHVPH
                     GTAHDFRRAAITLLENMGISVEFSHHEGAPGQNEIDLRYADALSTADNIMTFRTVVKE
                     VALEQGVRASFMPKPFREHPGSGMHTHLSLFEGDTNAFHEAGAPY"
     gene            complement(876..1091)
                     /locus_tag="PLK64_15340"
     CDS             complement(876..1091)
                     /locus_tag="PLK64_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminopeptidase"
                     /protein_id="HPV81168.1"
                     /translation="MQYWYNVAKGVVENDDDRGPGEDVLGPYDTADEAARALESAHER
                     TEKWDAEDKAWNESGATTTWDDEELED"
     gene            1196..2059
                     /gene="map"
                     /locus_tag="PLK64_15345"
     CDS             1196..2059
                     /gene="map"
                     /locus_tag="PLK64_15345"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPV81169.1"
                     /translation="MPVSFASVAPHPVGPRRSVPVGIPRPEYVDKPAPARYQGSEVHD
                     AEMIERIRVAGRVAAQAMAAAAAAIAPGVTTDELDRIGHEFLLDHGAYPSTLGYRGFP
                     KSLCTSVNEVVCHGIPDSRRLQDGDIVNIDITAYLNGVHGDTDATYPVGEVDEESRLL
                     IERTHEAMMRGIKAVAPGRPINVIGRVISSYARRFGYGVVRDFTGHGIAEAFHSGLII
                     PHYDAAPSYATVMQPGMTFTIEPMLNLGTHEWDMWDDGWTVVTRDRRRSAQFEHTLVV
                     TETGSEILTLP"
     gene            2094..2846
                     /locus_tag="PLK64_15350"
     CDS             2094..2846
                     /locus_tag="PLK64_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPV81170.1"
                     /translation="MTSRVALGIDIGGSGIKGAPVDLDRGVLLADRVKIETPAESTPQ
                     AVAEIVRQIATHFIDTLPADAPIGVTIPGVVQHGVVRTAANIDKAWLDCPGEALFSAT
                     LGRRCLLVNDADAAGVAELRHGAAQDVEGLVIVTTLGTGIGIALLHNGVLIPNAELGH
                     LELDGHDAETRAAASAREREGLSWNKWAGRLQRYYTHLENLLWPDLFVVGGGVSRKSE
                     KFLPLLSLRTPIVPAELQNAAGIIGAAALAAD"
     gene            complement(2866..>3437)
                     /locus_tag="PLK64_15355"
     CDS             complement(2866..>3437)
                     /locus_tag="PLK64_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPV81171.1"
                     /translation="SGFEDLTDLDAILVTHQHADHLDQKRLPALVRANPGAAVHADPM
                     SAALLVEAGLAVGVLAQGADLHLGDVRVRPVGALHAFNHQWIPTVANVGVRLDAAGEP
                     SLFHPGDAYDGEPGDIDVLAVPINAPWCAVRDSIDFVRRLAPRSMVPIHDGLVAPPGR
                     ALYLTHIATNGGDDLTLHDLAGRGAVSIP"
BASE COUNT          545 a         1166 c         1172 g          554 t
ORIGIN      
        1 gtagggagca ccggcctcgt ggaaggcgtt ggtgtcgccc tcgaagaggc tcaggtgggt
       61 gtgcatgccc gacccggggt gctcgcggaa cggcttgggc atgaacgacg cccggacgcc
      121 ctgctcgagg gcgacctcct tgacgacggt gcggaaggtc atgatgttgt cggccgtcga
      181 gagtgcgtcg gcatagcgca ggtcgatctc gttctgcccg ggcgcgccct cgtgatggct
      241 gaactccacc gagatgccca tgttctccag gagcgtgatc gcggcccggc ggaagtcgtg
      301 ggcggtgccg tgtgggacgt ggtcgaagta gccgccgtcg tcgaccggca cgggggtgat
      361 ccgcttgccc ttcggcgggt gcagcaggaa gaactcgatc tcggggtggg tgtagaaggt
      421 gaaccccaag ttggcggccc ggctcaaggc ccggcgcagg acgtggcgcg ggtcggcgaa
      481 actgggctgc ccgtcagggg tgagcaggtc gcagaacatt cgcgcggtgc ccgggttctc
      541 cccgcgccac ggcagcacct ggaaggtcga cgggtcgggc ttaacgagca tgtcggcctc
      601 gtagacgcgg gcgaatccct cgatggccga gccgtcgaag ccgatcccct cggcgaaggc
      661 accctcaagc tcggcgggcg caaccgccac cgacttcaac gtcccgacga cgtcggtgaa
      721 ccacaaccgg acgaaccgga tgtcgcgctc ttcgatcgtg cgcaggacga actcttgctg
      781 acggtccatg tgacgatcct gccgcaccgg tgttaccggc gcgtttcagg ccccgacttt
      841 ggtcagtcca gggccagtac cggatgagcc cgggatcagt cctcgagttc ctcgtcgtcc
      901 cacgtcgtgg tggcgccaga ttcgttccac gccttgtcct cggcgtccca cttctcggtg
      961 cgctcgtggg cgctctccag ggcgcgggcc gcttcgtcgg cagtgtcgta cgggcccagc
     1021 acgtcctcgc ccggaccgcg gtcatcgtcg ttctccacga cgcccttggc cacgttgtac
     1081 cagtactgca cggcatacct cccggtcttg tgggaacgac agacacaccg tatgccgtcc
     1141 ccgcaacttc gcgcgcgtgc cccctgtctt cgcgcagatg catagactcc gcggtatgcc
     1201 ggtgagcttc gcgagcgtcg cgccgcaccc agtcggcccg cgccgcagcg tccctgtcgg
     1261 catccctagg ccggagtacg tcgacaagcc tgccccagcg cggtatcagg ggtccgaggt
     1321 ccacgacgcc gagatgatcg aacggatccg ggtggccggg cgagtggctg cgcaggcgat
     1381 ggcggctgcc gccgcggcga tcgcgcccgg cgtgacgacc gacgaactcg accggatcgg
     1441 gcacgagttc ctgctggatc acggggccta tccctcgacg ctcggctacc gcggattccc
     1501 caagtcgttg tgcacatcgg ttaacgaggt cgtgtgtcac ggcatccccg attcccggcg
     1561 gctgcaggac ggcgacatcg tcaacatcga catcacggca tacctcaacg gggtccacgg
     1621 agacaccgac gcgacctacc ccgtgggcga ggtcgacgag gagtcgcggc tgctcatcga
     1681 gcgcacccac gaggcgatga tgcgcgggat caaggccgtg gcgccgggtc gaccgatcaa
     1741 cgttatcggc cgggtgatct cttcctacgc aaggagattc ggctacgggg tcgtgcgcga
     1801 cttcaccggc cacgggatcg ccgaggcctt ccacagcggc ctgatcatcc cccactacga
     1861 cgccgcgccg tcctacgcca ccgtcatgca accgggcatg acgttcacca tcgaaccgat
     1921 gctcaacctc ggcacccacg agtgggacat gtgggacgac gggtggaccg tcgtcacgcg
     1981 ggaccggcgc cgttcggcgc agttcgaaca caccctggtg gtcaccgaga cgggtagtga
     2041 gattctcacc ctcccgtaga caagctcgcg cccccatagg gaagtatgtg cccatgacct
     2101 cgcgtgtggc cctcggaatc gacatcggcg gatcgggcat caagggagct ccggtcgacc
     2161 tcgaccgcgg ggtgttgctc gcagaccgag tgaagatcga gactccggcg gagtccacgc
     2221 cccaagcggt cgcggagatc gtccgccaga tcgctacgca tttcatcgac accctccccg
     2281 ctgacgcgcc gatcggcgtc accatccccg gggtcgtgca gcacggcgtc gtgcgcacgg
     2341 ctgccaacat cgacaaggcc tggctcgact gcccaggcga ggcgctgttc agcgccacgt
     2401 tgggtcggcg ttgcctgctg gtcaacgacg ccgacgccgc gggtgtcgcc gagttgcgcc
     2461 acggcgcggc ccaggacgtc gaaggtttgg tgatcgtcac gaccctcgga accggcatcg
     2521 gtatcgccct gctgcacaac ggcgtgctca tccccaacgc ggaactcggc caccttgagc
     2581 tcgacggcca cgacgccgag acccgcgccg cagccagcgc tcgcgagcgt gaggggttgt
     2641 cctggaacaa gtgggccggg cgtctgcagc gctactacac ccacctggag aacctcctct
     2701 ggccggacct gttcgtcgtc ggcggcgggg tctcgcgcaa gtcggagaag ttccttccgt
     2761 tgctgtcgct gcgcacgccc atcgtcccgg ccgagttgca gaacgccgcc ggcatcatcg
     2821 gcgctgccgc tctcgccgcc gactgaggcc gctacggcgg aactgttaag ggatggagac
     2881 tgcgcctcgc ccggcgaggt cgtgcagggt gaggtcatcg ccgccgttgg tggcgatatg
     2941 ggtgagatag agggctcgtc cgggcggagc gactaacccg tcgtggatcg ggaccatcga
     3001 gcgcggagcg agccggcgca cgaagtcgat ggagtctcgg acggcacacc acggggcgtt
     3061 gatcggcacg gccaacacgt cgatgtctcc gggctcgccg tcgtaggcat cgcccgggtg
     3121 gaacaggctc ggctcccccg ccgcgtcaag gcgcaccccg acgttggcga cggtcgggat
     3181 ccattgatgg ttgaacgcgt gcagcgcccc caccgggcgc acgcggacgt cgccgaggtg
     3241 caggtcagcg ccctgcgcaa gcactccgac ggccagcccg gcctcgacca gcaacgccgc
     3301 actcatcggg tcggcatgca ccgcggcccc ggggttggcc cggaccagtg ccggaagcct
     3361 cttctggtcg aggtggtcgg cgtgttggtg ggtgacgagg atcgcatcca ggtcggtgag
     3421 gtcctcgaaa cccgacg
//
LOCUS       DAOMZY010000364         3411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_26793_length_3411_cov_4.643623, whole genome shotgun sequence.
ACCESSION   DAOMZY010000364 DAOMZY010000000
VERSION     DAOMZY010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26793_length_3411_cov_4.643623"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..448
                     /locus_tag="PLK64_15360"
     CDS             233..448
                     /locus_tag="PLK64_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81172.1"
                     /translation="MSTQWRWDLLDEAGNPMVGPEPASPSFPTQSEAESWIGEVWPEL
                     VDANIDAVTLREGETIVYGPMSLRPAP"
     gene            445..1767
                     /locus_tag="PLK64_15365"
     CDS             445..1767
                     /locus_tag="PLK64_15365"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPV81173.1"
                     /translation="MNPQLQARIDHLVADRQRAWRAPNVSVGLVRDGQLVHTVHVGAA
                     RLDPVTAATDDTPFMIGSVTKTFTAVVIMALRDEGRLDLDDRLDAHLPGISHGELTIR
                     LMLGHLSGLQREPVGRVWESMDNPDADRLLAELAQAEQVLAPHEVFHYSNLAFALLGQ
                     VIERLERRPWEDVVRQRVLHPLGMTRTGLTPAADRARGYLVHPFSGVATEEPVADLKA
                     TAPMGGLWSTVADLSRYAAFVADPDPAVLAPATVATMCRPQVILDPDAWTLGYGLSFG
                     MVRRGDRIYVGHGGAMPGFLTGLRVSRPDRLGAVVWANSSAGAEPIVLAADLLDLVLD
                     AEPTAASPWTPEASVPELDEFLGSWWSEGEELVFSVRDGRLWCAPRGAGALFESRFIP
                     DGPDRFRVDLGRERGEALDVERDASGRISRMYLATYAVTREPLAFAEL"
     gene            complement(1827..1910)
                     /locus_tag="PLK64_15370"
     CDS             complement(1827..1910)
                     /locus_tag="PLK64_15370"
                     /inference="COORDINATES: protein motif:HMM:NF022686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3043 domain-containing protein"
                     /protein_id="HPV81174.1"
                     /translation="MYAVMRAYQIRRSRLPRPMIKRGEYPV"
     assembly_gap    1925..2024
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2025..2557)
                     /locus_tag="PLK64_15375"
     CDS             complement(<2025..2557)
                     /locus_tag="PLK64_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3043 domain-containing protein"
                     /protein_id="HPV81175.1"
                     /translation="MFWSKKTQPAPVADDRPERPGAKNRPTPKRRDQEAANKHPVVNT
                     DRKAAARSDREARRVAMAESRAAMVTGDESKMPPRDKGPIKRYIRDYVDARFSLGEAL
                     LPMMLLALLLSFIKEVWALTTVFLLVYGLLFMSIADVFFMWRKIKAGVAAKFGADAMP
                     AGAMMYAVMRAYQIRRSR"
     gene            complement(2622..>3411)
                     /locus_tag="PLK64_15380"
     CDS             complement(2622..>3411)
                     /locus_tag="PLK64_15380"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPV81176.1"
                     /translation="QLARHVPDFSRMRVMPGTDVPLPPPMTVLDVGAGQGTQAIRLAR
                     LGHRVTALEPDPRMRDVAAAALALEEPGVRSRMDILAGGLGTLEAAVGWTEYDVVLCH
                     GVFMYLPSAEPAVSELTSHVASHGLLSLVVRNSAGLALRPALQGRWDEVGVLLDEVAA
                     AEEEGRDPLYTNEIGVRARADDVGTLAEVCRVAGLLPVAHYGVRLVSDQVPLDTPTPT
                     PEELEALVTTEVRLGDLDPYRQVCTLAHLVFSPDLRDGLNRPNL"
BASE COUNT          486 a         1157 c         1146 g          522 t
ORIGIN      
        1 ccggggccct gggtgatcgg cttccaggac gacaacccgg gcgcgcggga gttctggacc
       61 gcggttgcca ccgaggcggt cggggatgcg tggacgttgg aacaccaccc cgtgccgggc
      121 ctgccgcacc tgccgccgga ctcgttcatc gggttcacgg tccccggctg agcacgtgga
      181 gggctcgcca tcgcgcattc cagcgcccac cccgtgggtt agcgtggggg ctatgagcac
      241 gcagtggcgc tgggacctcc tcgacgaggc cgggaacccc atggtcggac ctgagccggc
      301 ctctccgtcg ttccccaccc aatccgaggc cgagtcgtgg atcggtgaag tctggcctga
      361 gctcgtcgac gccaacatcg acgcggtgac cttgcgcgag ggcgagacga tcgtctacgg
      421 cccgatgtcg ctgcgacccg ccccgtgaac ccgcagcttc aagcccgcat cgaccatctc
      481 gtcgccgacc ggcaacgggc gtggcgtgct ccgaacgtct cggtcgggct ggtgcgcgac
      541 gggcagctcg tccacaccgt gcatgtcggc gccgcccggt tggacccagt cacggccgcc
      601 accgacgaca caccgttcat gattgggtcg gtcaccaaga ccttcacggc cgtggtgatc
      661 atggcgttgc gcgacgaggg gcgtctggat ctcgatgatc ggctcgatgc tcacctgccc
      721 ggaatctccc acggagagct gacgatccgc ctcatgttgg ggcatctgtc cggtctgcag
      781 cgcgagcccg tcggtcgggt ctgggagagc atggacaacc ccgatgccga ccggttgctc
      841 gctgagttgg cgcaggcgga gcaggtgctg gcaccgcatg aggtctttca ctactccaat
      901 ctcgccttcg ccctgcttgg ccaggtgatc gagcggctcg aacgccgccc ctgggaagac
      961 gtcgtccggc agcgcgtcct acacccgctg gggatgaccc gcacggggct cactccggcg
     1021 gccgaccggg cgcgcggcta cctcgttcac cccttctccg gggtggccac cgaggagccg
     1081 gtggccgacc tcaaggccac ggcccccatg ggcgggctct ggtcgacggt cgccgacctc
     1141 agccggtatg ccgcgttcgt cgccgacccc gacccggccg tcctcgcacc ggccaccgtc
     1201 gccacgatgt gccggccgca ggtcatcctt gaccccgacg catggacgct gggatatggc
     1261 ttgtccttcg ggatggtccg acggggcgac cgcatctacg tcgggcacgg gggagcgatg
     1321 ccgggcttcc tcaccgggct tcgggtcagt cggcctgatc ggctcggcgc cgtggtgtgg
     1381 gccaactcct cagccggtgc cgaacccatc gtcctggccg ccgacctgct cgacctggtc
     1441 ctcgatgccg agcccacggc ggcatccccc tggacgcccg aggcgtcggt gccggagctc
     1501 gacgagttcc tcgggtcgtg gtggtccgag ggcgaagagt tggtgttttc cgtccgcgac
     1561 gggcgcctct ggtgcgcgcc gcgtggcgcg ggggcgctgt tcgagagccg gttcatcccc
     1621 gacgggcctg accgcttccg cgtcgacctc ggtcgcgagc gcggcgaagc cctcgacgtg
     1681 gagcgcgacg cgagcggccg gatctcccgt atgtatctcg cgacctacgc cgtcacccgc
     1741 gaaccgctgg ccttcgccga actctgaggc gccgagcgcc aaaccgggtc agatcggtca
     1801 gtccaggtga gtccacgcag cccaggtcag accgggtatt cgccccgctt gatcatcggg
     1861 cgcggcagcc ggctgcgccg gatctggtag gcgcgcatca cggcatacat catcgcgccg
     1921 gcggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncggctg cgccggatct
     2041 ggtaggcgcg catcacggca tacatcatcg cgccggcggg catcgcgtcg gcgccgaact
     2101 tcgccgccac ccccgccttg atcttgcgcc acatgaagaa cacgtcggcg atcgacatga
     2161 acaacaaccc gtagaccagc aggaagacgg tggtcagcgc ccagacctct ttgatgaacg
     2221 acaagagcag cgcaaggagc atcatcggca gcagggcctc gcccaggctg aaccgggcgt
     2281 cgacgtagtc gcggatatag cgcttgatgg ggcccttgtc gcggggcggc atcttcgact
     2341 cgtcgccggt gaccatcgcg gctcgcgact cggccatcgc cacccggcgg gcctcgcgat
     2401 cactgcgggc cgccgccttg cggtcggtgt tgacgaccgg gtgcttgttg gctgcctcct
     2461 ggtcgcgccg cttgggcgtg ggtcggttct tggcgccggg acgctcgggg cggtcgtcgg
     2521 cgacgggggc gggctgcgtc ttcttgctcc agaacacgag ggcaactgta tgccgtccag
     2581 ccgcggcacc cgtccgtccg acaactgtcg gagggtccgg ttcacaggtt tgggcggttc
     2641 agcccgtctc ggaggtccgg cgagaagacc agatgggcca aggtgcacac ctgccggtag
     2701 gggtcgagat cgccgaggcg cacctcggtg gtcaccagag cctcaagctc ttccggggtt
     2761 ggagtcgggg tgtcgagggg cacctggtcg gagaccagtc ggaccccgta gtgagcgacc
     2821 gggagcaggc cggccactcg gcatacctcg gccagtgtgc ccacgtcatc ggcccgagcg
     2881 cggaccccga tctcgttggt gtagagcgga tctcggccct cttcctccgc ggcagcaact
     2941 tcgtccagca acacgccgac ctcgtcccat cgaccctgca acgcaggccg cagggccagc
     3001 ccggccgagt tgcgcacgac cagtgagagc aggccatgtg aggcgacgtg cgacgtgagc
     3061 tccgaaacag caggctccgc ggaggggagg tacatgaaga cgccgtggca tagcacgacg
     3121 tcgtattcag tccagcccac cgcggcctcc agggtgccga gaccgcccgc gaggatatcc
     3181 atccgcgagc gcaccccagg ctcctccaga gctagggcgg ccgccgccac gtcgcgcatc
     3241 cgcgggtcgg gctcgagcgc agtcacccgg tgcccgagtc gggccagccg gatcgcctgg
     3301 gtgccttgac cggcaccgac gtcaaggacc gtcatcggtg gcggcagcgg gacgtccgtc
     3361 cccggcatga cccgcatgcg tgagaagtcg ggaacgtgcc gggcaagctg g
//
LOCUS       DAOMZY010000365         3403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_26871_length_3403_cov_4.209976, whole genome shotgun sequence.
ACCESSION   DAOMZY010000365 DAOMZY010000000
VERSION     DAOMZY010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26871_length_3403_cov_4.209976"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PLK64_15385"
     CDS             complement(<1..331)
                     /locus_tag="PLK64_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81177.1"
                     /translation="MTRADRLGFWVVLTAISGLLIENGTSSSPWLFFNPFGLLYVLPL
                     YGLHVVLLTRAVLAAPRPSLPMLYVAGALLGLYEAYVTKVLWSPTWGSTDGAVVGGLH
                     VFQTAVLV"
     gene            complement(394..891)
                     /locus_tag="PLK64_15390"
     CDS             complement(394..891)
                     /locus_tag="PLK64_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HPV81178.1"
                     /translation="MADSSFDVVSKYDKQEIANAVNTAAKEIANRYDFKNVGASIELS
                     GESIVMKANSEDRVKAILDVLQTWLIKRKVSLKHLDGADKTPRLSGKEYRLDVGLKEG
                     ISQEVAKKLTKLIRDEGPKTVKAQIQGDELRVSSKSKDDLQSVMNLLRGHDDIEVALQ
                     FTNYR"
     gene            1021..1104
                     /locus_tag="PLK64_15395"
     tRNA            1021..1104
                     /locus_tag="PLK64_15395"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1055..1057,aa:Tyr,seq:gta)
     gene            2374..2826
                     /locus_tag="PLK64_15400"
     CDS             2374..2826
                     /locus_tag="PLK64_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81179.1"
                     /translation="MVRAKLILVDLVSGLLAALLLVVWASPLREWGDPFSLSWGMAAW
                     LAVAFIGGMAVGRAQARLADRALRSGPLSAIAVGLIAGLPVFVLTHAFIQFTRIMPVP
                     GGGDSLDPSLGELASGLAFWSAFVLGAMAGVGQAKGRPSHPAVAADRT"
     gene            3218..3373
                     /locus_tag="PLK64_15405"
     CDS             3218..3373
                     /locus_tag="PLK64_15405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81180.1"
                     /translation="MSGDRVVFVGEAMAAIVQGIAEIRSVIGQPPVVVGSLAVNCSRE
                     ARHTLSL"
BASE COUNT          512 a         1091 c         1192 g          608 t
ORIGIN      
        1 gcacgagcac cgcggtctgg aagacgtgca gcccgccgac gaccgcaccg tcggtcgacc
       61 cccacgtcgg tgaccaaagg actttggtga cgtaggcctc gtacaggccg aggagggcgc
      121 cggcgacgta gagcatcggg aggctcgggc gcggggccgc gaggacggcg cgggtcagca
      181 ggacgacatg cagcccgtag agcggcagga cgtacagcag cccgaacggg ttgaagaaca
      241 gccacggcga ggaggacgtg ccgttctcga tgagcagccc actgatcgcg gtgaggacga
      301 cccagaagcc gaggcggtcg gcgcgggtca tggctggcta cggcgtcggg gctgtcgtgg
      361 gctggtcgtg gctggccgtg aagtcggcgc gggtcagcgg tagttggtga actgcagggc
      421 gacctcgatg tcatcgtggc cgcgcaggag gttcatgacc gactgcaggt cgtccttgga
      481 cttgctcgac acccgcaact cgtcgccctg gatctgcgcc ttgaccgtct tggggccctc
      541 gtcgcggatg agcttggtga gcttcttggc gacctcctgg ctgatgccct ccttgaggcc
      601 cacgtccagg cggtattcct tgccggacag gcgcggcgtc ttgtcggcgc cgtcgaggtg
      661 cttgagcgag accttgcgct tgatcagcca ggtctgcagg acgtccagga tcgccttgac
      721 ccggtcttcg gagttggcct tcatgacgat gctctcgccg gacagctcga tcgaggcgcc
      781 gacgttcttg aagtcgtagc ggttggcgat ctccttggct gccgtgttga cggcattggc
      841 gatctcctgc ttgtcgtact tgctcacgac atcgaacgag ctgtcggcca tgggtcggtt
      901 ctcctgttgg tcggggcgcg ggccgggtgg gttggggtcg ggccgcgccg ggttggtggg
      961 tggctggcag gcaagtttcg tatgccgccg ggctggctgc tatcctcgca tgcgcaccac
     1021 ggcaggttgc ccgagcggcc aatgggagcg gactgtaaat ccgtcgcgaa agcttccaag
     1081 gttcgaatcc ttgacctgcc accacgtgca ccgaaggcct ctgaccagcg gaaacgccgg
     1141 tcagaggcct tcgctgtatc cccgtggggg ccatcgctcc gggcttgtgc ttgggaggtc
     1201 tcacggcgag ggtgcaagcc tctgacctgc gcaaacgagc ggcgagccgg cccctgtgca
     1261 gaatgcgtgc agaagttggg cctcgctcgg cgtaccagcc ggtgtcgcgc gccgttgacg
     1321 tgcagcaaca ccggggaggg ggtatgcaat cggagcggaa tccgggaccg tccccattgt
     1381 catggcgtcg gagtcggagg ctgccgactg gcacagctcg gtcgacgttg gggtgcaatg
     1441 ctcaagcgca tctcggccgt gtaggggccg caaggcgctt gaggccggcg ccccctagcc
     1501 ggcgatgcgg tcgcgggtct ttctcgtgac acgagggcac cgatacgtcg cgccgccacc
     1561 cgctttcgca gcgcaccgac cggagcccgt gctcatcgac aggtatatgc aggcgaaccc
     1621 accagaccta tcgggcgaga cggcatgccg tgaaatcggc aaccgaacgg gccctgtggt
     1681 ggggacccgc gcaggcgcac ccgcttgagg tactcgtgcg cggtcgcgat cttcacgccc
     1741 atgtgctgcg caacgcgctt catcggcagg cccgtggcgt agaggcggag cgcctccacc
     1801 tcgcgcgggg ccaggtgggg caccgaccag ctcgggtctc cctcgagcgc gacggcccag
     1861 tgcggctcag gtagggctct cccgccgcgg tgcatcatca ggcccgctcg gcatcgcccg
     1921 gtagtccccg aaccgtcgac gtgtggagga aacggccgcc ctttacgttc cctgcatcgg
     1981 caccgacacg atcaaggaga acaatgatgc ctatcaactc ccacaaacct acgctccgca
     2041 gtgcggcggc aagcctcgcc gtggcggcac ttgcaacgat cggcggcgtc gccgctgagt
     2101 ccactgcagc agttccatgg aactgcccca ttagcggttc cggcatcagc cgtgggagaa
     2161 ctaacaaatt gcagaagctg gagatgtcgg gtctgcgcgg gccagccaag tacgccgcag
     2221 cagaagtcac ctgggccggc gaggaccggc cttcgcatcg gcctgaggca gccctgtggc
     2281 ggcgctgtga cgcaagatga gcaggccgcc ctctggatga cgtgtgggca ctgccacccc
     2341 ggtgcgcgcg ggccgtgcca gtacggtgac gctgtggttc gggcgaaact cattctggtc
     2401 gacctggtaa gcggcctgct cgccgccttg ttactcgtcg tgtgggcgag tcccctgagg
     2461 gagtgggggg atcccttcag cctcagttgg ggaatggccg cttggctggc cgtggcgttc
     2521 attggcggca tggccgtggg ccgggcgcag gctcgcctcg ccgaccgagc gctacgttca
     2581 gggccgctca gtgccatagc cgtcgggctg atcgccgggc ttccggtttt cgtgcttacc
     2641 cacgcgttca tccaattcac gaggatcatg cccgttcccg gaggtggaga ctcgctcgac
     2701 ccttcccttg gggagttggc tagtggcttg gccttctggt cggcgttcgt actcggcgcc
     2761 atggccggtg tcggccaagc gaagggacga ccgtcgcacc ccgcggtcgc cgctgaccga
     2821 acctgaccac ggccgcagag gccggtgacc cgatgtccag acgcccccgc ccagcacgcg
     2881 gcgctatgac gcacctcgtc tgcgggcgct tgaacgctaa gcgggctaat cgctgcatgg
     2941 agggtggtat ctccagtcct caacttcttc gtccctgacc agtctgtcct tcggcgcgcg
     3001 gtgaccctgc ttggcggagt tgctttctcg gcggaggccg cggcgacact tcgggttccc
     3061 ccggtggcgg ctgcgacggc gcatcgcgtt gatcgggcca atagtcggct tggatggccc
     3121 ctggtgcaac ccttgttcgt cgccctcgat ctggccctcg acgtgggccg cggccgggag
     3181 atcctgcagg ggtggacccc ggatgatggg tggccgcgtg tctggtgacc gcgtcgtctt
     3241 cgtcggtgag gcgatggcag ccatcgtgca aggcatcgcc gaaatccgat ccgtcattgg
     3301 ccaaccgccg gtcgtcgtcg gcagccttgc cgtgaactgc agccgggagg cacggcatac
     3361 cctctcgctg tgaaccgcgc agacctgacg gccgccgagc aac
//
LOCUS       DAOMZY010000366         3390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_27012_length_3390_cov_5.332234, whole genome shotgun sequence.
ACCESSION   DAOMZY010000366 DAOMZY010000000
VERSION     DAOMZY010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27012_length_3390_cov_5.332234"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PLK64_15410"
     CDS             complement(<1..380)
                     /locus_tag="PLK64_15410"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HPV81181.1"
                     /translation="MTPPGDPTGDPTVDPTVDPAESDAATVTVDARGLRCPLPVLRLA
                     QALAQQPPGTAVRLLATDPAAHTDVAAFVRMRGLLLLGVRVEGEHTAYLVRGVSATAP
                     EAAPSGPPAADPGMPPAAPPARPTT"
     gene            complement(377..1579)
                     /locus_tag="PLK64_15415"
     CDS             complement(377..1579)
                     /locus_tag="PLK64_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPV81182.1"
                     /translation="MAYPSFGPSTEPPFRHSRRALLDAAQGPVHPAARETMAAAWEAG
                     WADPRRLHAEGRRARRLLDEARSVLAQGLGVAPETLSFTPGGPTALRLGLEGLRYAAR
                     RTGARLVAATVEHSAILLPGRHAAAAAGDPGQFGPVPVDALGRVDLAAWATALDSPGT
                     AVAALQSANGEVGTRQPLAAAHEECRRRGIPLLVDATAGLGRDATPESYDVLVGDARS
                     FAGPPGIGLLVLPPRTRWRLPGPPTEVEGGRVEIEPVLPLALGAAEAWAQTVAARDAD
                     ARAARALVDTIRAAAAAIPDTEVVGDPLDRLPHVVTCSFLLADGEALVTELDRRGIAV
                     ASGSACTSSTLEPSHVLAAMGALTHGNIRVTLPLESVSPQRTDDVAALVAALPDAVAA
                     VRAQLQAR"
     gene            1678..2589
                     /gene="coxB"
                     /locus_tag="PLK64_15420"
     CDS             1678..2589
                     /gene="coxB"
                     /locus_tag="PLK64_15420"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HPV81183.1"
                     /translation="MRKHADTSARVTRRGLTIAGLAASATLLLSSCANVNSSASSGWL
                     PEGTAGAHVTSETGRITTLWVGTWIAGLAVGALVWGLAIWCMIAYRRKKDDPELPPQL
                     RYNVPIELLYTVVPVLMIAVLFYYTARDEAALMDVSTKPDYTINVVGKQWSWDFNYVD
                     DNVYSSGVHSELNPNGGVTGTPQPVLYLPVNKRVEFVLNARDVIHSFWVPAFLVKLDM
                     IPGKTNRLQITPTQVGEFQGKCAELCGAYHSQMLFKVKIVSQEDFDAEMAKLKSLGQE
                     GQLPNTLNREPLETKDLKMVPTPTGSK"
     gene            2589..>3390
                     /locus_tag="PLK64_15425"
     CDS             2589..>3390
                     /locus_tag="PLK64_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HPV81184.1"
                     /translation="MTATIERPEAAARVQHPRPRPGQTFYSWITTTDHKKIGNLYFIT
                     SFVFFLLAGAMALMIRAELTAPGLQVVDNPEQFNQLFTMHGLIMLLLFATPLFAGFAN
                     ALVPLQIGAPDVAFPRLNMFAYWLYVLGGLVAALGFLTPQGAASFGWFMYVPLSNSIY
                     SPGLGTDLGVFGLTLTGFGTILGSVNFITTIVCMRAPGMTMFRMPVFTWTVLVTALLA
                     LIIFPVLAAALFGLGGDRRFGMHIFDVEAGGAMLWQHLFWFFGHPEVYV"
BASE COUNT          500 a         1168 c         1190 g          532 t
ORIGIN      
        1 gtcgttgggc gtgcgggagg tgccgcgggc ggcatacctg ggtctgctgc gggaggccct
       61 gaaggcgccg cttccggcgc cgtggcgctg actccgcgca cgaggtatgc cgtgtgctcg
      121 ccctcgacgc gcacgccgag caggagcagc ccccgcatcc ggacgaaggc cgcaacgtcg
      181 gtgtgcgccg cggggtcggt cgcgagcagc cgcactgcgg tgcccggcgg ctgttgcgcg
      241 agggcctgag ccagccgcag caccggcagg ggacagcgca gcccgcgggc gtcgacggtg
      301 accgttgcgg cgtcgctttc ggccgggtcg acggtggggt cgacggtggg gtcgccggtg
      361 gggtcgccgg gcggcgtcat cgggcctgca actgggctcg gaccgcagca acggcgtcgg
      421 gaagtgcggc cacgagcgcg gcgacatcgt cggtccgctg gggcgacacc gactcgaggg
      481 gcaaggtcac ccggatgttg ccgtgggtga gggcgcccat cgccgcgagc acgtggctcg
      541 gctcaagggt gctcgatgtg cacgccgagc cggacgcgac cgcgatgccg cgccgatcga
      601 gttcggtgac gagggcttcg ccgtcggcca agaggaacga acacgtcacg acatggggga
      661 gtcggtcgag cggatctccg accacctcgg tgtcagggat cgctgccgcc gcggcccgga
      721 tcgtgtcgac gagggcgcga gccgcgcggg cgtcggcgtc gcgtgcggcg accgtctgcg
      781 cccaggcttc cgcggcaccc agcgccaagg ggagcaccgg ctcgatttcg acccggccgc
      841 cctccacctc ggtgggcggg cccggcaggc gccagcgggt ccgcggcggg aggaccagca
      901 gaccgatacc cgggggaccg gcgaacgagc gggcatcccc gaccagaacg tcgtaagact
      961 cgggggtggc gtcccggccc agacccgccg tggcatcgac aagcaggggt atgccgcgcc
     1021 gccggcactc ctcgtgagcg gccgcgaggg gctggcgggt gccgacctcg ccgttggcgc
     1081 tctgcagcgc ggccacggcc gtgccggggg agtccagggc cgtcgcccac gcggcgaggt
     1141 cgacccgccc gagggcatcg accgggaccg gaccgaactg tccagggtcg cccgcggccg
     1201 cggcggcgtg tcgcccgggc agcaggatcg ccgagtgttc gaccgtggcg gcgaccagtc
     1261 gcgctccggt gcggcgggcg gcataccgca gaccctccag cccgaggcgc aaggcggtcg
     1321 gccccccggg ggtgaagctc agtgtctcgg gggcgacgcc gaggccctgc gcgagcacgg
     1381 atctggcctc atcaagcagt cgccgggccc ggcggccctc ggcgtgcagg cgccgcggat
     1441 cggcccaccc agcctcccag gccgcggcca tcgtctcgcg tgccgccggg tggacgggac
     1501 cctgcgcggc atcgaggagc gcccggcggg agtgcctgaa aggaggctcc gttgagggcc
     1561 cgaatgaggg atacgccacg tcgagaggtt atcggcaccc ctgccgtgat ccgtcgattc
     1621 catcgcggta gggtggcccc acacacgagc agtcactctg tattcggaag gtgcccgttg
     1681 cgtaagcacg cagacacctc cgctcgcgtc acgcggcggg gattgaccat tgccgggctg
     1741 gcggcctccg ccacgctcct cctttccagt tgtgccaatg tcaacagcag cgcctccagt
     1801 gggtggctgc cggagggcac ggctggagcg cacgtcacga gcgagacggg tcggatcacc
     1861 acgctgtggg tgggcacctg gatcgccggc ctcgcggtcg gcgccctcgt ctggggcttg
     1921 gcgatctggt gcatgatcgc ctaccggcgc aagaaggacg atccggaact gccgccccag
     1981 ttgcgctaca acgtgccgat cgagttgctc tatacggtcg tgccggtcct catgatcgcc
     2041 gtgttgttct actacacggc gcgagacgag gccgcgctca tggacgtctc gaccaagccg
     2101 gactacacga tcaatgtcgt cggcaagcag tggagctggg acttcaacta cgtcgatgac
     2161 aacgtctact ccagtggcgt ccactccgag ctcaacccca acgggggagt gacggggacg
     2221 ccgcagccgg tgctctacct gccggtcaac aagcgcgtcg agttcgtcct caacgcccgc
     2281 gacgtcatcc actcgttctg ggtgcccgcg ttcctggtca agctcgacat gatccccggc
     2341 aagaccaacc ggttgcagat caccccgacc caggttggcg agttccaggg caagtgcgcc
     2401 gagttgtgcg gtgcctacca ctcgcagatg ctcttcaagg tcaagatcgt ctcgcaggag
     2461 gacttcgacg ccgagatggc caagctcaag agcctcggcc aggagggtca gctgcccaac
     2521 acgctcaacc gtgagcccct cgaaaccaag gacctcaaga tggtcccgac cccgaccggg
     2581 agcaagtgat gactgcgacg atcgagcgcc ccgaggccgc ggcccgggtg cagcacccgc
     2641 ggccacggcc agggcagacg ttctacagct ggatcacgac gaccgatcac aagaagatcg
     2701 gcaacctcta cttcatcacc tcgttcgtct tcttcctgct cgcaggggcg atggctctga
     2761 tgatccgggc cgagttgact gctcccggcc tgcaggtcgt cgacaaccca gagcagttca
     2821 accagctgtt caccatgcac ggcctgatca tgttgctgct gttcgcgacg ccgctgttcg
     2881 ccggtttcgc taacgcgctg gtgccgctgc agatcggtgc accggacgtc gcgttcccgc
     2941 ggctgaacat gttcgcctac tggctgtacg tgctcggtgg cctcgtggcg gcgctcggct
     3001 tcctcacccc ccagggtgcg gcgtcgttcg gctggttcat gtatgtgccg ctgtccaact
     3061 cgatctacag ccccggcctc ggcaccgacc tcggcgtctt cggcctgacc ctcaccggtt
     3121 tcggcaccat cctcgggtcg gtcaacttca tcacgacgat cgtctgcatg cgcgcaccag
     3181 gcatgacgat gttccgtatg ccggtcttca cctggaccgt gctcgtgacg gcgctgctcg
     3241 cgctcatcat cttcccggtg ctggcggccg cgctcttcgg gctcggtggc gaccggcgct
     3301 tcgggatgca catcttcgat gtcgaggccg gcggtgcgat gctctggcag cacctgttct
     3361 ggttcttcgg ccacccggag gtctacgtcc
//
LOCUS       DAOMZY010000367         3349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_27507_length_3349_cov_7.304493, whole genome shotgun sequence.
ACCESSION   DAOMZY010000367 DAOMZY010000000
VERSION     DAOMZY010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27507_length_3349_cov_7.304493"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1343
                     /locus_tag="PLK64_15430"
     CDS             114..1343
                     /locus_tag="PLK64_15430"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPV81185.1"
                     /translation="MVSTASDSLRVRFVGPLAPHSAGLADEFSRLGYSVKSAVIVVQF
                     AAHLSRWLALEQMELSEVTDAVIDRFLAVRRAEYTSHYSRRALQPLLAYLRCEGLVPP
                     EVKPVAVSGIEVLLTRYRRYLVSDRGLSGPVADAYSHWVTPFVTDVLGGTDPVARVAV
                     VTAADVAGFLASRLPSMTRKTAQMTACSLRSFFRFAHAEKLTGVMLADAIPAVAHRRL
                     SGLPEPLAQAQVTALLGVCDRTTRVGRRDFAVITLLCRLGLRCGEVSGLLLSDLDWRA
                     GVVTIHGKGGRVDRLPLPVDVGQAIVAYLRDGRPETTARTVFVRAVAPFTPLASASLS
                     CIVARAAERAGLGTVHAHRLRHTMASRVLNAGASLEEVSHLLRHASPATTAIYAKTDL
                     TRLATISRPWPLSGRVS"
     gene            1340..2281
                     /locus_tag="PLK64_15435"
     CDS             1340..2281
                     /locus_tag="PLK64_15435"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPV81186.1"
                     /translation="MTSSLEGVSDLVSQYLRVRRALGYKLNNAEYILDRFVAYLEVRG
                     ADRVSVADAVGFATAPPERAGRTQALRLSAVRCFTRWAHCQNPDIEVPPARLLPARPS
                     RVAPYIYTARQLDALLEAAGRLQPEGRAATYQALIGLMAATGIRTGEAIGLDTSSLDR
                     HTGILTVIGKYGKRRMLPLHGTVTDALTGYLSQRRHFAGTDTDHALLVSTRGTRLHPS
                     TVHATFRALTVEAGLGTVSSACRPRLHDLRHTFAVSTMLDAYRRGDDPAVVLPVLSTW
                     LGHTEPRDTYWYLTGTAELLEAAATRLDTTDKERALP"
     gene            2278..3264
                     /locus_tag="PLK64_15440"
     CDS             2278..3264
                     /locus_tag="PLK64_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007270300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPV81187.1"
                     /translation="MTGLAPLLQAFFTTRLTGQYGASVHTIAAYRDTWRLLLRYAATT
                     TGTAPAALNLTQINSDLISGFLTYLETDRGNTIRTRNARLAAVHSLFTYAAYQYPEHA
                     DIIRRVLAIPAKRAPRTEITYLTPPEVTALLNAPDPSTATGRRDHAMIQVAVTTGMRV
                     AELTALRVADTHLGTGAHVVCHGKGRKERITPLDRQTVRVLRNLISHSTPDAFVFPTR
                     DGTRMSHDAVTARLALHLVTATAACPTLTGKRITPHVLRHTAAMRLLNAGIDSTVIAL
                     WLGHESIETTQVYLHANIKTKEDALARTRPTGASPGRYTVTDDTLLAFLDGL"
BASE COUNT          570 a         1211 c         1013 g          555 t
ORIGIN      
        1 ttttatgccg atgtcctggt tatgccgatg tgagcgggtc agtgcgctgg tgacgtcggc
       61 ataattcggt ggctggcggg atgctggttc ttccctgatt ggaaggacaa gtgatggtgt
      121 ctactgcttc tgattcgttg cgtgttcgct tcgtgggtcc gctggctccg cattcggcgg
      181 ggctggctga tgagttctcg aggctcggct actcggtgaa gtcggcggtg atagtggtcc
      241 agttcgcggc gcatctgtcg cgctggcttg cgttggaaca gatggagctg agcgaggtga
      301 ccgacgcggt gatcgatcgc ttcctggctg tccgccgcgc tgagtacacc agccattact
      361 cacggcgggc gctgcagccg ttgctggcgt atctgcgctg cgaggggctg gtgccgcctg
      421 aggtcaagcc ggtggcagtc tccgggatcg aggtgctgtt gacccgttac cgtcgttacc
      481 tggtcagtga tcgtgggttg agtggtccgg tcgcggacgc gtactcgcat tgggtgacgc
      541 ctttcgtcac cgacgtcctc ggcggcacgg accccgtagc cagggttgct gtggtgacgg
      601 cggccgatgt ggccgggttc ctcgcttcac ggttgccgtc gatgacgcgg aagacagcgc
      661 agatgaccgc atgttcgctg cggtccttct tccggttcgc gcacgccgag aagctcaccg
      721 gtgtgatgct cgcggacgcg attcccgcgg tcgcgcaccg acgactgtcg gggttgccgg
      781 agccgttggc gcaggcgcag gtgacggcgt tgctcggggt ctgcgaccgg acaactcggg
      841 tcgggcgacg ggacttcgct gtgatcacac tgctgtgtcg gctcgggcta cgctgcggcg
      901 aggtgtccgg gttgctgctg tcggatctgg actggcgcgc tggagtcgtg acgatccacg
      961 ggaaaggcgg ccgcgtcgac cgactaccgc taccggtcga cgtcggtcag gcgatcgtcg
     1021 cctacctgcg ggacggccgg ccggagacca ccgcccggac cgtgttcgtc agagccgtgg
     1081 ctcccttcac gcctctggct agcgcgagtc tgagttgcat cgtcgcccga gcggcggaac
     1141 gcgctgggct gggcacggtc cacgcgcacc ggttgcgtca caccatggcg agccgggtcc
     1201 tcaacgccgg ggccagcctt gaggaggtct cgcatctgtt gcggcacgcg agcccagcca
     1261 ccacggcgat ctacgccaag accgatctga cccggctggc gacgatcagc cggccctggc
     1321 cgttgtcggg gagggtgtca tgacctcgtc actggaaggc gtgtccgatc tggtcagcca
     1381 gtatctccgg gtgaggcgcg cgttgggcta caagctcaac aacgccgagt acatcctcga
     1441 tcggttcgtc gcctacctgg aggttcgagg cgctgaccgg gtgagcgttg ccgatgctgt
     1501 cgggttcgcg accgcgcccc ccgagcgcgc cggccggacg caggcgctgc ggctgtcagc
     1561 ggttcgctgc ttcacccgct gggcacactg ccagaacccc gacatcgaag tcccaccggc
     1621 gaggcttctc ccggcacgcc cctccagggt cgccccctac atctacaccg cacggcagct
     1681 cgacgcgctg ctggaagccg cagggaggct gcagcctgag ggacgagccg cgacctacca
     1741 ggctctgatc gggttgatgg ccgccacggg gatccgcacc ggcgaggcga tcggtttgga
     1801 taccagcagc ctcgaccggc acaccgggat ccttaccgtc atcggcaagt acggcaagcg
     1861 ccggatgctt cccctgcacg gcaccgtcac cgacgcactg accggctacc tcagccagcg
     1921 acgccacttc gctggcaccg acaccgacca cgcactgctt gtctccaccc gtgggacacg
     1981 gctgcacccg tccactgttc acgccacgtt ccgggctctc accgtcgagg ccgggctggg
     2041 aaccgtgtcc tcggcctgca ggccacggtt gcatgacctg cgccacacct tcgccgtctc
     2101 cacgatgctt gacgcatacc gacgaggcga cgaccccgcc gtggttctgc cggtcctgtc
     2161 gacctggctc ggtcacacgg aaccccgcga cacctactgg tatctcaccg gcaccgcgga
     2221 actcctcgaa gcggcagcca cacggctcga caccaccgac aaggaacgag ccctgccatg
     2281 accggactcg ccccgctcct acaagcgttc ttcaccaccc gactgaccgg tcaatacggc
     2341 gccagcgttc acaccatcgc cgcttaccgc gacacctggc ggctgctgct gcgatacgcc
     2401 gccacaacaa ccgggacagc gcccgccgcc ctcaacctga cgcagatcaa cagcgacctg
     2461 atcagcggat tcctcaccta cctagaaacc gaccgcggga acacgatcag gacccgcaac
     2521 gccagactgg ccgcagtcca ctccctattc acctacgccg cttaccagta ccccgagcac
     2581 gccgatatca tccgcagagt cctcgcgatc cccgccaaac gagccccacg caccgagatc
     2641 acctacctca ccccacccga ggtcacagca ctgctcaacg cacccgaccc ctcaacggcg
     2701 acaggacgcc gcgaccacgc catgatccag gtcgcggtca ccaccggcat gcgagtcgcc
     2761 gagctcaccg cactccgagt cgccgacacc cacctcggga ccggcgccca cgtcgtctgt
     2821 cacggcaagg gacgaaagga gcgcatcaca ccactggacc gtcaaaccgt ccgcgtgctg
     2881 cgcaacctga tctcccactc cacccctgac gcgttcgtgt tcccgacccg cgatggaaca
     2941 aggatgagtc acgacgccgt cacagcacgc ctcgcgctcc acctcgtcac cgcgaccgcc
     3001 gcctgcccca cgctcaccgg gaagagaatc accccgcacg tcctacgcca caccgcagca
     3061 atgcgcctcc tcaacgcagg catcgacagc accgttatcg cgctctggct cggccacgag
     3121 agcatcgaga ccacgcaggt ctacctgcac gccaacatca agaccaagga agacgccctc
     3181 gcacgcactc gacccaccgg cgccagcccc gggcgctaca cagtcaccga cgacaccctc
     3241 ctcgccttcc tcgacggcct ctgattatgc cgacatccca accgccccaa cccaacccac
     3301 gagcgcactg acccgctcac atcggcataa ccaggacatc ggcataaaa
//
LOCUS       DAOMZY010000368         3331 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_27693_length_3331_cov_4.570208, whole genome shotgun sequence.
ACCESSION   DAOMZY010000368 DAOMZY010000000
VERSION     DAOMZY010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27693_length_3331_cov_4.570208"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /locus_tag="PLK64_15445"
     CDS             <1..134
                     /locus_tag="PLK64_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HPV81188.1"
                     /translation="DYHQQYLHKNPNGYCNHGPFMGLSCPVGVANLPAQTDVMPPSE"
     gene            complement(234..383)
                     /locus_tag="PLK64_15450"
     CDS             complement(234..383)
                     /locus_tag="PLK64_15450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81189.1"
                     /translation="MPPPPTVMPAWPCVNPAHAGSRLLPGTVVVIVLVLQALSRVEVT
                     LLFGQ"
     gene            complement(1123..2187)
                     /locus_tag="PLK64_15455"
     CDS             complement(1123..2187)
                     /locus_tag="PLK64_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81190.1"
                     /translation="MNTTTLDPASAEHVATEAPTDPAADPYALAEAAVDKARAVVSQR
                     LRERDDADAALPGLRSRLARGDDSVTASDLAAAAMIEDRAELLLKAAESSLKRTTAAG
                     PFRPSTATLLVDAVATALAINVDDVALVDAPNAVGKVDSASLRQAAPTTAAESLTGAV
                     ACREVEVYYHRSPKFRAADWVALDDALTKAGFYAEVASRGSSERNGIVTDRAVINVRA
                     AFPAVLVLPGKTDPSNWDSNHFVNVVLAEADHAVPPHVDLSRTGVVGRRSVRGSEVVT
                     VVTATIKGKGGRDTTGQALPVGGVMAAIAAAPQALIGRPFSGLGHLAKVEVIPTADTT
                     PERRGELTHRIAATFTATAA"
     gene            complement(2591..3151)
                     /locus_tag="PLK64_15460"
     CDS             complement(2591..3151)
                     /locus_tag="PLK64_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81191.1"
                     /translation="MRTDDLAVEVEHETADAGEDGREVQGFVIHPEWLLGGPLRRSPA
                     RWLADWELDKARPETRRLAAAFVRGGFTECGAQVKPKSATYIARAAGVSSRSAERFLS
                     KGRTVGKLTPTGLRVGLVEGKRHTPLYHLTQPTGLRMEDVIPDEMRVEVAAPVAKFPA
                     ESAWPSSPWEAVTEAAPEWARAGSVG"
BASE COUNT          600 a         1064 c         1172 g          495 t
ORIGIN      
        1 aggactacca ccagcagtac ctgcacaaga acccgaacgg gtactgcaac cacggaccat
       61 tcatggggtt gtcctgcccg gtcggcgtgg cgaacctgcc tgctcaaacc gacgtcatgc
      121 ccccgagcga gtagcagact ccgcacgttc tcaagtagca gcacctctga cagggaggcc
      181 ccgccgtcac aacatcgtga gcagcggggc caacctgtag agcaggggca gggctactgc
      241 ccgaacagca gcgtgacctc tacccgtgag agcgcctgta ggaccaggac gatcacgacg
      301 accgtgccgg ggagcaggcg actaccggcg tgcgcggggt tcacgcacgg ccaggcgggc
      361 attactgtgg gcgggggtgg catagagcct cctacgatcc gagtcccccg gtgcctgtaa
      421 acaggtctca cgtgtcaagg caccgggggc gaggactgtc ctcgtaatca cgactgtagg
      481 ccgggagcag gacaagcggg gatgccccga tgagggtgcc gccggcaacc gtcacagaga
      541 gttccctaat gtgtcgtcaa gtaacgtcaa gtgacgtaat gcgacaccgg agcgtcacgg
      601 gtattcgctc ggagctcggc gcgtctcggg cgtgtcgcgc cctctggggc ccgcgctcgt
      661 gggcggtcgg ccggtggctc gattcgatga gtgaggctgc gagagcgcca cggacgggac
      721 gccatggccc ttgtcacagt gttcgtgagc acggcaggca ccccaggggt atgacccccc
      781 agcgcagcgg ctcccccact gcggagtttt agcgtccagg cgacagcgca ggttctaggg
      841 tcgatacccc taggggtacg tgactggctc tcaggtggca cgagggcgtg acctgtcggt
      901 atgccgtcgg cagcccgtcg ggagcctcag ggaaaccctg cccaaacagg gggaaataga
      961 cgaaaacccc caccccaccc gcggccagga agcgaaccgg gggtgaggcg ggggtatcgg
     1021 agtcgcggca ggaccaccat ccaagaatgg gcctatcgcg ctctggcgtg ctcccggtca
     1081 tcgacaacca cgacgacgca ccgggagcgg gatcaggtgg acctatgcgg ccgtagcggt
     1141 gaacgtggcg gcgatccggt gggtgagctc gccccggcgc tcaggggtgg tgtcggcggt
     1201 ggggatgacc tcgaccttgg ccaggtgacc gagaccagag aacggccggc cgatgagggc
     1261 ctgaggtgcc gcggcgattg cagccatcac cccgcctacc ggcagagcct gcccggtggt
     1321 gtctcgacca cccttgccct tgatcgtggc ggtcaccacg gtgacgacct cggagccgcg
     1381 caccgatcga cgaccgacga cgccggtacg cgacagatcg acgtgagggg gcacggcgtg
     1441 gtcagcctcg gccaggacca cattgacgaa atggttggag tcccagttgc tcgggtccgt
     1501 cttccccggc agcacgagca cggcagggaa cgcggcacgg acgttgatga cggcacggtc
     1561 ggtaacgatg ccgttccgct cggagctgcc gcgggaggca acctcggcgt agaacccggc
     1621 cttggtcaag gcgtcgtcga gcgccaccca gtcagcggca cggaacttgg gcgagcggtg
     1681 gtagtagacc tcgacctcac ggcaggcgac agcacctgtc agtgactcgg ccgcggtggt
     1741 cggggcagcc tgtcgcagag acgcgctgtc caccttgccc acggcgttgg gagcatccac
     1801 gagggccacg tcatcgacgt tgatggccag ggccgtggca acggcatcca cgagcagggt
     1861 ggcggtcgac ggacggaagg gacccgcggc agtggtccgc ttgagcgagg actccgcagc
     1921 cttgagcagg agttcggccc ggtcctcgat catcgcagcc gcagccaggt cggaggccgt
     1981 cacgctgtca tcgccgcgag cgagtcggga gcgcagacca ggaagcgcag cgtcggcatc
     2041 gtcgcgctct cgcagccgct gagagacgac ggcacgggcc ttgtcgacgg cagcctcagc
     2101 gagcgcgtac gggtccgcag cggggtccgt gggggcctcg gtggcgacgt gctcggcgga
     2161 ggcagggtca agggtggtgg tgttcatggt tgtggttcct tcactggtag cggatgccag
     2221 gacaggcatc ggcaggtggt gcagacgcca gaccgaacta tcggagaacg gtggcggtgg
     2281 tgatgtcgag caatggcgac ggagggacga tggcgacagc caccgaccga gcgcagcgag
     2341 ggaggtgcag cggcgacgac ggagcgaagc gacgaaggag tcagggggaa gaggcaggga
     2401 caaaccagta gccggtagca gttcgtcccc gctggggtcg aagaccccca cccacaaaca
     2461 gttgatcgac aagcagttag ccgttggtcc ctacctacct acctgtcacc gccacgacgg
     2521 gtggggcgct ccgcgccccg ccagggcgaa cggacaagcg ggctggtggc ttgcccttag
     2581 ttaccaatac ttatccgaca cttccggcgc gagcccattc gggagcggcc tcggtgactg
     2641 cctcccacgg actcgacggc cacgccgatt cggcggggaa cttggcgact ggggcggcga
     2701 cctcgacgcg catctcgtca gggatcacat cctccattcg gaggccggtc ggttgggtca
     2761 ggtgatagag cggggtgtgc cgcttgccct cgaccaggcc aaccctcagg ccggtcggcg
     2821 tcaacttgcc cacggttcgg cccttgctga ggaaccgctc cgcagaccgc gagctgacgc
     2881 cagcagctcg agcaatgtag gtggctgact tgggcttgac ttgggcgccg cactcggtga
     2941 aaccaccgcg cacgaacgcc gccgccagtc gccgcgtctc aggtcgcgcc ttgtcgagct
     3001 cccagtctgc gagccaccgt gccggcgagc gtcggagcgg accaccgagc agccactcgg
     3061 gatggatcac gaacccttgg acctcgcgcc cgtcctcgcc agcgtcggca gtctcgtgct
     3121 ctacctctac ggccagatcg tcagttcgca ccgttcgccc cttcccgtcc catggctgtc
     3181 atggaccgtt ctaggtcggt gaactgacac aggtctgtgt gtcccgtcat cggggaggcc
     3241 ctgacgagag cgcacgccgg taggcgcacg atgtggagca cgtcccgtcg atgacacggc
     3301 cgggaggcat ggacccaccg cagaccaggc a
//
LOCUS       DAOMZY010000369         3328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_27723_length_3328_cov_4.975558, whole genome shotgun sequence.
ACCESSION   DAOMZY010000369 DAOMZY010000000
VERSION     DAOMZY010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27723_length_3328_cov_4.975558"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..594
                     /locus_tag="PLK64_15465"
     CDS             238..594
                     /locus_tag="PLK64_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HPV81192.1"
                     /translation="MSVQDQTTTTTHGVVLTDVAASKVKSLLAQEGRDDLRLRIGVQP
                     GGCSGLIYQLYFDERTLDGDALRDFDGVEVVVDRMSAPYLDGATIDFADTIEKQGFTI
                     DNPNAGSSCACGDSFS"
     gene            complement(692..1228)
                     /locus_tag="PLK64_15470"
     CDS             complement(692..1228)
                     /locus_tag="PLK64_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81193.1"
                     /translation="MSAYEWFRIRRTMLAYDYWLDVEGVTGRARKDLRAELVGNLTDA
                     TAAVGYPAARRSLGSVRTLATAAAPDRDSRPRWVVGSYAAIVVFAIGAFAALGLITAW
                     ADGVMAAGGGQGLWIGHPFFLPWVEITVRQGETLQILATFNPWSVVVPPLVAFLIGSR
                     IWRLATRQSSPTPAAQVR"
     gene            complement(1230..1577)
                     /locus_tag="PLK64_15475"
     CDS             complement(1230..1577)
                     /locus_tag="PLK64_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPV81194.1"
                     /translation="MTTHDPQMIKGVLGLLLLQLLDEGDGYGYGIVTRLQENGFEDLA
                     EGTVYPALARLEAAGHLDSYLLRSAAGPARKYYRITAGGRTELIRARRDWDALVERVR
                     LLTPTQLTAQTGA"
     gene            1680..2666
                     /locus_tag="PLK64_15480"
     CDS             1680..2666
                     /locus_tag="PLK64_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HPV81195.1"
                     /translation="MRVAVTGSIATDHLMTFGGKFADSLVVEQLDKISLSFLADDLQI
                     RRGGIAANIAFGMANLGQHPVLIGSVGKDFDDYRSWLERHGVDCQSIHVSGKSHTARF
                     VCTTDVDMAQIATFYPGAMTEARGIELKPVADRVGGIQLVLIGPNDPVAMQRHTDECR
                     TRGIPFAADPSQQLAWLDGRGIRDLIEGAAYLFTNEYEAHMIAQKAGWDERDLADAVS
                     VQVTTKGKDGATIRQKGHDPIHVTVVPGVQPLDPTGVGDAFRGGFLTGLAAGLDHRRC
                     AELGSTLAAYVIETVGTQEYSLTTSAFVQRIDEAYGADAATAIRSHIRCPKP"
     gene            2690..>3328
                     /gene="aat"
                     /locus_tag="PLK64_15485"
     CDS             2690..>3328
                     /gene="aat"
                     /locus_tag="PLK64_15485"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HPV81196.1"
                     /translation="MPLDPGPSRYAFDIERADAGEDLVGAGADLEPATILAAYRAGAF
                     PMGLGRHGAGPMGWWSPDPRGVLRPEDLRVSRSLRRSCARLQVTVDTAFDAVVRGCAD
                     PDREGRWITRPVTRAYTRLHALGWAHSVEVWSGDQLVGGLYGIAIGGLFAGESMFHRV
                     TDASKVALVALRELITADGDPRRIIDVQWVTPHLASLGVREVPRAAYLGLLRE"
BASE COUNT          530 a         1142 c         1139 g          517 t
ORIGIN      
        1 caggacgcct acgccgaggc cgtcgcccgc acctggtcac ccccaccgcg atcctgagcg
       61 accgagcagt cagggcctgc gcgcagcgag acgggaccac acgaagtagg ggaatccgcg
      121 ctgcacgtcg tacattgggt cgacgccgta cgcggggaac aaccgtcgcc cggccgcggt
      181 tgtcccagtc aacgtcttag tctcgaccgc cagcacccga ccacgaggaa ccccactatg
      241 agcgtccagg accagaccac caccacgacc cacggcgtcg tcctcaccga cgtcgcggcc
      301 tcgaaggtca agagcctgtt ggcccaggag ggccgcgacg acctgcgcct tcgcatcggc
      361 gtccagcccg gtggctgctc aggtctcatc taccagctct acttcgacga gcgcaccctt
      421 gatggcgacg cgctgcgcga cttcgacggg gtcgaggtcg tcgtcgaccg gatgagcgcc
      481 ccttacctcg atggcgcgac gatcgacttc gccgacacca tcgagaagca gggcttcacc
      541 atcgacaacc ccaacgcagg cagctcgtgc gcatgcggtg acagcttcag ctgacctgct
      601 cccgcagagc gaacgaaggc ccccggacca ttgaggtctc gggggccttc gcgtatgccg
      661 tgacgcgagg tcccgacacg acgtccagac gtcagcgcac ctgggcagca ggcgtggggg
      721 aggactgccg ggtggccagt cgccagatcc gcgacccgat gaggaaggcg acgagcgggg
      781 ggaccaccac gctccagggg ttgaaggtgg cgaggatctg cagcgtctcg ccctggcgga
      841 ccgtgatctc gacccagggg aggaagaagg gatgcccgat ccacagcccc tgaccgccgc
      901 ctgccgccat gacaccgtca gcccacgccg tgatcaggcc gagcgcggca aaggctccga
      961 tcgcgaagac gacgatcgcg gcatacgacc cgacgaccca gcggggccgg ctgtcccggt
     1021 ccggcgctgc ggcagtggcg agggtgcgga cactgcccag cgaccgccga gcggccgggt
     1081 aaccgaccgc ggcggtggcg tccgtgagat tcccgacgag ttccgcgcgc aggtccttgc
     1141 gggctcgacc tgtcacgcct tcgacatcga gccagtagtc gtaggcgagc atcgtgcggc
     1201 ggatccggaa ccactcgtat gcggacatgt caggcccctg tctgtgcggt gagttgagtc
     1261 ggcgtgagga gtcggacgcg ctcgacgagt gcgtcccagt cgcgacgggc gcggatcagc
     1321 tccgtgcggc ctccggcggt gatccggtag tacttgcggg ccggcccagc ggcggagcgc
     1381 aggaggtagg aatcgaggtg gccggccgcc tccaaccggg ccagcgcggg atacacggtg
     1441 ccttcggcga ggtcctcgaa gccgttctcc tgcaatcggg tgacgatgcc gtagccgtag
     1501 ccgtcaccct cgtcgagcag ttgcagcagg agcagcccga gcaccccttt gatcatctgt
     1561 gggtcgtgcg ttgtcatggc tatgagagta tgactagtac ctagacatgt caagtagtgg
     1621 tgattcctca gttccggccc tgcgatccag ggggtatgcc gtccactagc gttgcgggca
     1681 tgcgcgtcgc cgtgacgggg tccatcgcca ccgaccacct gatgaccttc ggtgggaagt
     1741 tcgccgactc cctcgtcgtc gaacagctgg acaagatctc gttgtccttc ctcgccgacg
     1801 acctgcagat ccgtcgcggg ggcatcgccg ccaacatcgc cttcggcatg gccaacctcg
     1861 gccagcaccc cgtgctgatc ggctcggtcg gcaaggactt cgacgactat cgatcctggc
     1921 tggagcgcca cggggtggac tgccagtcga tccacgtgtc cgggaagtcc cacaccgccc
     1981 ggttcgtgtg cacgaccgat gtcgacatgg ctcagatcgc gacgttctat cccggcgcga
     2041 tgaccgaggc ccgtggcatc gagctcaagc cggtggccga tcgggtcggc gggatccagt
     2101 tggtcctcat cggccccaac gacccggtgg cgatgcaacg ccataccgac gagtgccgca
     2161 cgcgcggcat accttttgcg gccgatccgt cccagcaact ggcgtggttg gacgggcgcg
     2221 gtatccgtga cctcatcgag ggcgccgcct acctgttcac caacgagtac gaagcgcaca
     2281 tgatcgccca gaaggccggc tgggacgagc gcgacctcgc cgatgccgtc agcgtccagg
     2341 tgacgacgaa gggcaaggac ggcgcgacga tccgccagaa gggccacgac ccgatccacg
     2401 tcactgtcgt cccgggcgtc cagccgctgg acccgacggg ggtcggtgac gccttccgcg
     2461 gcggcttcct caccgggttg gcggcaggcc tggaccaccg tcggtgcgcc gagctcggct
     2521 cgacgctcgc ggcatacgtc atcgagacgg tcggcaccca ggagtattcg ctgacgacca
     2581 gcgccttcgt ccagcgcatc gacgaggcgt acggagccga cgccgccacg gcgatccgct
     2641 ctcacatccg ctgtcccaaa ccctgacgga cccgggtcag ccatccgcca tgccgcttga
     2701 tccggggccg tcgcgctatg ccttcgacat cgagcgcgca gacgcggggg aggatctcgt
     2761 cggcgccggt gccgatctcg agccggcgac catcctcgct gcctaccgcg ccggggcgtt
     2821 ccccatgggg ttgggtcggc atggtgcggg gccgatgggg tggtggtcgc cggatccgcg
     2881 cggcgtgctc cgacccgagg acctgagggt cagccgctcg ctgcggcgct cctgcgctcg
     2941 gctgcaggtg acggtcgaca cggccttcga tgcggtcgtc cgcggctgtg ccgaccccga
     3001 ccgcgagggc cggtggatca cccgtccggt gacccgggcc tacacccgac tgcatgcgct
     3061 cgggtgggcg cactcggtgg aggtctggtc cggggatcaa ctcgtcggcg ggctctacgg
     3121 catcgcgatc ggtgggttgt tcgcggggga gtcgatgttc caccgggtga ccgacgcctc
     3181 caaggtcgcg ctggtcgcct tgcgcgagct catcacggcc gacggcgatc ctcggcggat
     3241 catcgacgtc cagtgggtca cgcctcacct ggcgtcgttg ggcgtgcggg aggtgccgcg
     3301 ggcggcatac ctgggtctgc tgcgggag
//
LOCUS       DAOMZY010000370         3323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_27771_length_3323_cov_5.130967, whole genome shotgun sequence.
ACCESSION   DAOMZY010000370 DAOMZY010000000
VERSION     DAOMZY010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27771_length_3323_cov_5.130967"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PLK64_15490"
     CDS             <1..694
                     /locus_tag="PLK64_15490"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV81197.1"
                     /translation="DSGMSILRVFMMQLFNIIAVTTSVFGATYATNKEYGVGMAASVY
                     LWIPILGNICAVAVIPFVGNLSDKIGRRPLIMFATITCGLLSFGYLWSVSQKNVPLAI
                     AFSLLMWGTVYQGQNAVYPAFYPEQFPTRTRVTGMAIAHNMGTAISSAMAFVLAWVAP
                     PGTQNVWLVVGAITMGICIISFFGAYFSPETSRFRSEDLGVPGAKPVPEAEYLRLRQA
                     AIDSAKAQKVAA"
     gene            807..989
                     /locus_tag="PLK64_15495"
     CDS             807..989
                     /locus_tag="PLK64_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81198.1"
                     /translation="MSSTTNPIVGLVVGVLLIPLGWIVARDGGASMGPFGWLLLGVGV
                     ASTVGNGLLMLRARRR"
     gene            1078..1734
                     /locus_tag="PLK64_15500"
     CDS             1078..1734
                     /locus_tag="PLK64_15500"
                     /inference="COORDINATES: protein motif:HMM:NF037007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPV81199.1"
                     /translation="MSNSPKAARKQRDAARTQADLLTVATEEFARIGYFGARVDEIAA
                     KSATTKRMIYYYFGDKEGLFTAVLEKAYADIRETEQALDLAHMPPLDALTVLIRHTFD
                     YHEAHPGLARLVASENALDASHLRLSQRAAGVNLPIIALVADLLARGRSEGSIVRDVD
                     PLDLHLMMTSMALFRITNAPTIEATFGLRMGTHEHKERQIALVTEMVLTWLTTPDSRD
                     "
     gene            1806..3083
                     /locus_tag="PLK64_15505"
     CDS             1806..3083
                     /locus_tag="PLK64_15505"
                     /inference="COORDINATES: protein motif:HMM:NF020031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP citrate lyase citrate-binding
                     domain-containing protein"
                     /protein_id="HPV81200.1"
                     /translation="MNLTGMLYGAKLLQFAGFPAAEVLGPAATEEEIKDLISRHGEIL
                     IKPVFKGGVGKKGKAGLIGRAKDLPTALREKERLYFAEHQVGTTLAKANGVTYEAMIP
                     AEHEVYFSLTDSTHFRAPTMTITHHGGVAIEELDKSLVASVPFEALTGMRAFVVSNAL
                     SQLQAPSEIVSPLVQHLPSLWELFHEFGMTTLELNPIRMRADKRGRLTPVACDFKCAF
                     DRDDPRVARLDLPRSLFSSSYSDFEHEVNQLRTYQGQSDVYVVNEKGTILAPTFGGGA
                     NSLVSEMLGDDAIISSDFGGNPPYVKMKELARICYKYWLPQTNVLFIIGGKANNTDIF
                     ETFRAMADALREHYATHGPTPLYAVIGRGGPNLIRGMGVLRDTFESLKIPYKMFGFDS
                     DMSEVINYARAADAWMKASGREQIARQQQALAG"
     gene            3108..>3323
                     /locus_tag="PLK64_15510"
     CDS             3108..>3323
                     /locus_tag="PLK64_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81201.1"
                     /translation="MYKKGVGEFKFYVGISSLAQIATKDDRVCVLNILGGESSDVTPV
                     SHEYSGGNVVFGTSPGKGGSHMPTNLGD"
BASE COUNT          563 a         1170 c         1024 g          566 t
ORIGIN      
        1 cgacagcggc atgtcgatcc tgcgggtgtt catgatgcag ttgttcaaca tcatcgccgt
       61 gacgacctcg gtcttcggtg ccacctatgc gacgaacaag gaatacggcg tcggcatggc
      121 ggccagcgtc tacctgtgga tccccatcct cggcaacatc tgcgcggtcg cggtcatccc
      181 gttcgtcggc aacctctcgg acaagatcgg tcgtcgaccg ctcatcatgt tcgcgacgat
      241 cacctgtggg ctgctgtcct tcggctacct ctggtcggtg agccagaaga acgtcccgct
      301 ggccattgcc ttctcgctgc ttatgtgggg cacggtctac cagggccaga acgccgtcta
      361 cccggccttc tacccggagc agttccccac tcgcacccgg gtgaccggaa tggcgatcgc
      421 ccacaacatg ggcaccgcga tctcttcggc gatggcgttc gtcctcgcct gggtggcacc
      481 gcccggcacg cagaacgtct ggcttgtcgt cggagccatc acgatgggta tctgcatcat
      541 ctccttcttc ggtgcctact tctcgccgga gacctcccgc ttccgcagtg aggacctcgg
      601 cgtccccggt gccaagccgg ttcccgaggc ggagtacctc cggctgcgtc aggctgcgat
      661 cgactcggcg aaggcccaga aggtcgccgc ttagtcggcg gaccgccgcg tgagcggcat
      721 accgttgtgc ccttggcccg tgcgccgggc gggacgttct cccacgtccc gcccggtggg
      781 ccccaccccc acacctggag cccactatga gctcaaccac caaccccatc gtcggtctcg
      841 tcgtcggggt cctgctcatc ccgctcgggt ggatcgtcgc ccgtgacggc ggcgcctcca
      901 tgggcccgtt cggttggctg ctgttgggcg tcggagtggc cagcaccgtc ggcaacgggc
      961 tgctcatgct gcgagcacgg cggcgctgag gccggtatgc cgccgcctgc cccggccagc
     1021 tctgcgcgcc ccgtcggctc gcctccgaca cctggggtcc ggcctacagt tggatccgtg
     1081 tcgaattccc ccaaggccgc ccgcaagcag cgcgatgccg cccggaccca ggccgacctg
     1141 ctgaccgtcg ctaccgagga attcgcgcgg atcggctact tcggggctcg cgtcgacgag
     1201 atcgccgcca aatcggccac gaccaagcgg atgatctact actacttcgg tgacaaggag
     1261 gggctgttca ccgcagttct ggagaaggcc tacgccgata tccgggagac cgagcaggcc
     1321 ctggacctgg cccacatgcc gcccttggac gcgctcaccg tgctcatccg gcataccttc
     1381 gactatcacg aggcccatcc ggggctggcc cgtctggtgg cctccgagaa cgccctggac
     1441 gcctcgcacc tgcgcctgtc ccagcgagcg gccggggtca acctgccgat catcgcgctg
     1501 gttgcggacc tgctcgcgcg cggtcgctcc gagggcagca tcgtgcgcga cgtggacccg
     1561 ctggacctgc acctcatgat gacgtccatg gcgctgttcc gcatcaccaa cgcacccacc
     1621 attgaggcga ccttcgggtt gcggatgggc actcacgagc acaaggaacg ccagatcgct
     1681 ctcgtgaccg aaatggtgct cacctggctc accacgcctg actcacggga ctagcgtccc
     1741 gggatgggtc cggccggaag gcttaccctg tcaaaggatc aacggcgacc cgctggagta
     1801 acaggatgaa tctcaccgga atgctctatg gagcgaaact tcttcagttc gcgggcttcc
     1861 ccgcggcgga ggtgttgggc ccggcagcaa ccgaggaaga gatcaaggat ctcatctccc
     1921 ggcacggtga gatcctcatc aaaccggtct tcaagggtgg cgtcggcaag aagggcaagg
     1981 ccggcctgat cggccgggcc aaggacctgc cgaccgcgct gcgcgagaag gaacggctct
     2041 acttcgccga gcatcaggtc ggcacgacgc tcgccaaggc caacggggtg acctacgagg
     2101 cgatgatccc cgcggagcac gaggtctact tctccctcac cgactcgacc cacttccggg
     2161 cccccacgat gacgatcacc caccatggcg gcgtcgccat cgaggagttg gacaagtcgc
     2221 tcgtcgcgtc ggtgcccttc gaggcgctca ccggcatgcg cgccttcgtc gtctcgaacg
     2281 ccctgtcgca gttgcaggcg cccagcgaga tcgtctcgcc gctcgtgcag cacctgccca
     2341 gcctgtggga gctcttccac gagttcggga tgaccaccct cgagctcaac ccgatccgga
     2401 tgcgcgcgga caagcgcggc cggctcacgc cggtggcctg cgatttcaag tgcgccttcg
     2461 accgcgacga cccccgggtg gcccgcctcg acctgccgcg ctcgctgttc tcctcgagct
     2521 actccgactt cgagcacgag gtcaaccaac tgcgcaccta ccaaggccag agcgacgtct
     2581 acgtcgtcaa cgagaagggg acgatcctcg ccccgacctt cggcggcggc gccaactcgc
     2641 tcgtgtccga gatgctcggc gacgacgcga tcatctcctc ggacttcggc ggcaacccgc
     2701 cctacgtcaa gatgaaggaa ctcgcgcgga tctgttacaa gtactggctg ccgcagacca
     2761 acgtgctgtt catcatcggc ggcaaggcca acaacaccga catcttcgag accttccggg
     2821 ccatggccga cgccctgcgc gagcactacg cgacgcacgg gccaacgccc ctgtatgccg
     2881 tgatcggacg cggcgggccg aacctcatcc gggggatggg cgtcctgcgg gacaccttcg
     2941 agagcctcaa gatcccctac aagatgttcg gcttcgactc agacatgtcc gaggtcatca
     3001 actacgcccg cgccgctgac gcctggatga aggcctcggg gcgcgagcag atcgcccgcc
     3061 aacagcaggc tttggccggc tgacgcggcg acgaaggaga cctgcagatg tacaagaagg
     3121 gcgtcggcga gttcaagttc tacgtcggca tcagttcact ggcccagatc gccaccaagg
     3181 acgaccgggt gtgtgtgctc aacatcctcg gaggtgagtc gagcgatgtc accccggtga
     3241 gccatgagta ctccgggggc aacgtcgtct tcggcacctc gcccggcaag ggcgggtcgc
     3301 acatgccgac caacctgggc gac
//
LOCUS       DAOMZY010000371         3315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_27891_length_3315_cov_6.851227, whole genome shotgun sequence.
ACCESSION   DAOMZY010000371 DAOMZY010000000
VERSION     DAOMZY010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27891_length_3315_cov_6.851227"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PLK64_15515"
     CDS             <1..404
                     /locus_tag="PLK64_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81202.1"
                     /translation="PMGTDHTCPLHLAGDRLCYKGCGCRCDDCTEMHNWSYRGPRPAA
                     PEMYLQDLSDGNCAGFPQFTELPDAAQYRACITCPVMAICPQPTPWPRGECCREGHDV
                     EHRHPNSKHCTECERERGRRDKSIGRRKKVA"
     gene            625..1398
                     /locus_tag="PLK64_15520"
     CDS             625..1398
                     /locus_tag="PLK64_15520"
                     /inference="COORDINATES: protein motif:HMM:NF025107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV81203.1"
                     /translation="MPVRLPRLHQAPSAGPAVRRRARTSGGGSGVSIRLTSQVWDSGR
                     YEQTALLVLLALADQANDEGVCWPAVSTIAARARCSARYVRTLLGEFEKDGWLSIEHR
                     DGKPSMYRLTIPTPEPQITPEPQITPEPQMRTPLNPSSATPEPQITQTIKNHQRTTNT
                     GREDVAGDFEAWYAAYPKKVARALALKAYRAARKKVDAQTLLAGVDVARLMARGRQYC
                     PNPASWLNQERWLDEHDQPEHEQSNVYRLPDGGQIERFW"
     gene            1395..2609
                     /locus_tag="PLK64_15525"
     CDS             1395..2609
                     /locus_tag="PLK64_15525"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPV81204.1"
                     /translation="MNPLDPADAELSLLACIFQQPRWLDVLDVTEADFYQPRHGELWN
                     RFAELHRDDSKPDPVNVGPHLSTLFIEVATNVLAVAGNAEHYAGVVKDASVRRSTLNA
                     LDGLRSRLNDLSIPLDQALAEVERALTKGADLEAAAAKLETLDEFLDRPRPPQRWVIP
                     DLLTVGDRLVLTGAEGFGKSVCMRQIGVMAACGLHPFALRNIPPQKVLLVDCENPEEI
                     MADVLGTLRDVARRRSVDAGDRFWLIRYPQGLDLSKPKDRLELHRQCMLIRPDLLLIG
                     PAYKLYVGGGNQREEDLARQVTSALDGLREEFGFALILEHHSPHASGTGERVVRPIGS
                     SLWLRWPEFGLGLAPNGETEEERARSFRDRKAVLRPWRGARADRPWPKFLHASQEPGA
                     LPWADPHALHHR"
     gene            2617..2823
                     /locus_tag="PLK64_15530"
     CDS             2617..2823
                     /locus_tag="PLK64_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81205.1"
                     /translation="MTDDPLMLMAEYATDTPYHPIQCVKFTNPLGIPDHAVVLVLDNG
                     YGTEDPPPIDFFRNLWDQHARKEL"
     gene            2827..3243
                     /gene="ssb"
                     /locus_tag="PLK64_15535"
     CDS             2827..3243
                     /gene="ssb"
                     /locus_tag="PLK64_15535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPV81206.1"
                     /translation="MSNSLTIVGRLAFDIEVRFSPSGDAVASGRVADTPRHKNRQTGE
                     WEDGETLWLGFSLWGKKAEALADAAKKGDLVLVTGTLTQRTFEGKHGEQRTVIEVKAT
                     EVAVVPRVQSGAGWSKDRSAAADPWQQPATNSEPPF"
BASE COUNT          634 a         1163 c         1026 g          492 t
ORIGIN      
        1 caccgatggg gactgaccac acctgcccgc tgcatctggc cggcgaccgt ctctgctaca
       61 agggatgcgg ctgccgctgc gacgactgca ccgagatgca caactggagc taccgaggac
      121 cacgaccagc cgcccccgag atgtatctgc aagacctgtc ggacggcaac tgcgccggct
      181 tcccacagtt caccgaactg cccgatgccg cccaatatcg ggcgtgcatc acctgcccgg
      241 tgatggcgat ctgcccgcag ccgacaccgt ggccgcgagg cgaatgctgc cgcgaagggc
      301 acgacgtcga gcatcgccat ccgaactcca agcactgcac ggaatgcgag cgggaacggg
      361 gtcggcggga taagtcgatc ggacgccgga agaaggtcgc ctagtgagcg cgaaaaacgg
      421 gccgctggtt cgcggtgtaa cgacggacgc ctgtacgatc gggggaagta aggacgaccc
      481 tgaggccggt gcgctaacac cgggggttcc tcagggcctc gccctcgatc cgcagcaacg
      541 gagaggacta ccgccaatgg tagacgaatc gctcgacccc tgtaagaccc gcgtccgcaa
      601 gctcgcgggc cgctggaact ggtcatgccc gtgcggttac cacggctcca ccaagcacca
      661 tcggctggcc ctgctgttcg ccgtcgagca cgcacatcgg gtggaggtag cggcgtgagc
      721 atccgactca cgagccaagt ctgggactct ggccgctacg agcagacggc cttgttggtg
      781 ctgctcgcac tcgcggacca ggccaacgac gaaggtgtgt gctggccggc agtctcgacg
      841 atcgcggcca gagcccggtg ctccgcgagg tacgtccgga cactcctggg cgagtttgaa
      901 aaggatggct ggctctcgat tgagcatcgg gatgggaagc cctcgatgta ccggctgacc
      961 atcccaaccc ctgaacccca gatcacccct gaaccccaga tcacccctga accccagatg
     1021 cgcacacccc tgaaccccag ttccgcgacc cctgaacccc agatcaccca aaccataaag
     1081 aaccaccaac gaaccaccaa cactggtcgc gaagacgtcg ccggcgactt cgaggcttgg
     1141 tacgcggcat acccgaagaa agtcgctcgg gctctggcat tgaaggccta ccgggccgct
     1201 cgcaagaagg tcgacgccca gaccctcctg gccggcgttg acgtcgcccg tttgatggcg
     1261 aggggccggc agtactgccc gaaccctgcg tcctggctga accaagagcg gtggctggac
     1321 gagcacgacc agccggagca cgagcagtcg aacgtttacc ggctccccga cggcgggcag
     1381 atcgagaggt tctggtgaac ccgctcgatc cggccgacgc cgagctgtcg ctcctggcct
     1441 gcatcttcca gcagccgagg tggctcgacg tgctcgacgt caccgaagcc gacttctacc
     1501 agccgcgcca tggtgagcta tggaaccggt tcgccgaact gcaccgcgac gacagcaagc
     1561 ccgacccggt gaacgtcggc ccccacttgt cgacgctgtt catcgaagtc gcaaccaacg
     1621 tcctcgccgt cgccgggaat gccgagcatt acgccggagt cgtgaaagac gcctccgtcc
     1681 ggcgctcgac cctgaatgcg ctcgacgggc tccgcagccg gctgaatgat ctgagcattc
     1741 ctcttgatca agccctggcc gaagtcgagc gggcattgac gaaaggcgct gacctggaag
     1801 cggcagctgc gaaactcgaa acactggatg agttcctgga tcgcccccga ccgccgcagc
     1861 ggtgggtgat cccggacctg ctcactgttg gcgatcgtct ggtgctgacc ggagccgagg
     1921 gttttggcaa atcggtctgc atgcggcaaa tcggagtaat ggccgcctgc ggattgcacc
     1981 cgttcgcgtt gcgaaacatt ccgccacaga aggtgctgct ggtggactgt gagaaccccg
     2041 aggagatcat ggctgacgtg ctcggcaccc ttcgggacgt cgcgcgccgc cgctcggtcg
     2101 atgccggcga ccggttctgg ctgatccgct acccgcaagg gctcgacctg tccaagccga
     2161 aggaccggct ggagctgcac cgccagtgca tgctgatccg ccccgatctg ctgctgatcg
     2221 gcccggcgta caagctgtac gtcggtggcg gcaaccaacg cgaagaagac ctcgcccgtc
     2281 aagtcacgtc tgcgctcgac ggactccggg aagagttcgg gttcgcgctg atcctcgaac
     2341 accactcccc ccacgcctcc ggcacaggcg agcgggtcgt ccggccaatc ggatcgtcgc
     2401 tatggctgcg ctggcccgag ttcgggctcg gcctcgcgcc gaacggcgag accgaggaag
     2461 agcgagcccg atcgttccgc gaccgcaagg ccgtcctacg gccgtggcgc ggagcccgcg
     2521 ccgatcggcc ctggcccaag ttcctgcacg catcccaaga gcccggcgcg ctgccgtggg
     2581 ccgacccgca cgcactccac caccgatagg agacccatga ccgacgaccc gctcatgctg
     2641 atggccgagt acgccaccga cacgccctac cacccgatcc agtgcgtgaa gttcaccaac
     2701 ccgctgggca tccccgatca cgccgtcgtc ctagtgctcg acaacggcta cggcaccgaa
     2761 gatccgccgc cgatcgactt cttccgcaac ctgtgggacc agcacgcccg aaaggaactc
     2821 tgaccaatgt ccaactcgct caccatcgtc ggccgcctgg ccttcgacat cgaggttcga
     2881 ttcagcccgt ccggtgacgc tgtggcgtcc ggccgcgtcg ccgacacccc gcgccacaag
     2941 aaccgccaga ccggcgaatg ggaggacggt gaaaccctgt ggctcggctt ctccctgtgg
     3001 ggcaagaagg ccgaagcgct cgccgacgcc gccaagaagg gcgacctcgt gctggtcacc
     3061 ggcaccctca cccaacgcac cttcgagggc aagcatggcg agcagcggac cgtcatcgag
     3121 gtcaaggcca ccgaggtcgc cgtcgtcccg cgtgtccagt cgggggctgg ttggtcgaag
     3181 gaccggtcgg cggccgccga tccgtggcag cagcccgcca ccaacagcga gccccccttc
     3241 tgaccgtgcg caccgtcatc accgacccgg cgcgaatccg cgccatcaga aaccaggagg
     3301 accaatgagc agcat
//
LOCUS       DAOMZY010000372         3299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_28081_length_3299_cov_5.688348, whole genome shotgun sequence.
ACCESSION   DAOMZY010000372 DAOMZY010000000
VERSION     DAOMZY010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28081_length_3299_cov_5.688348"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /locus_tag="PLK64_15540"
     CDS             <1..1278
                     /locus_tag="PLK64_15540"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HPV81207.1"
                     /translation="SVGFPLTEGVSDVAWPCSDTKYIAHFPETREIWSYGSGYGGNAL
                     LGKKCYALRIASAMARDEGWMAEHMLILKLTSPKGKVHYICGAFPSQCGKTNLAMLEP
                     TIPGWKAEMIGDDISWMRFGPDGRLYAVNPEFGLFGVAPGTGMSTNPMAMRTIEEGNS
                     IFTNVARTDDGDVWWEGISKEKPAHLIDWKNRDWTPDNGELSSHPNSRFTTPAKQCPM
                     IAAEYDNPDGVPISAILFGGRRASTVPLVYESRNWTHGTFVGATLSSETTAAATGAVG
                     VVRRDPMAMLPFIGYHAGDYMQHWINIGKQADESKLPKIYQVNWFRKDKDGHWLWPGF
                     GDNSRVLKWVVERLEGEADAVETPIGLVPAAGAIDTEGLDMAPDDIDKAVAVDLDEWR
                     AELPLIEEWFAKIGDRLPAQLHAELDGLKERLA"
     gene            1428..2111
                     /locus_tag="PLK64_15545"
     CDS             1428..2111
                     /locus_tag="PLK64_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPV81208.1"
                     /translation="MNPPPPTTGDADAGMPPAPAGSQPGRGIPPSPDDGRIPRPGGAP
                     APGSADAAFESLYRTCAPRVFAYVRRHVPPADCDDVVAEVFVVAWRRRLTVPPDPLPW
                     LIVVARNVLRGRRRLAVRADNVWWEAVRSLAAPAYSASPEASIDEREALLEGLARCSR
                     AEREALLLVAWDGLTAAQAADVAGCSTRAFTVRLSRARARLNGALGERPHRAAPLLLT
                     PSTPLAQEH"
     gene            2113..3123
                     /locus_tag="PLK64_15550"
     CDS             2113..3123
                     /locus_tag="PLK64_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81209.1"
                     /translation="MTTTDSLTRLATIRPDEDTLAHDWSPARRADVLARVHAATSDAP
                     AAATVTPLTRRRLFPARRLVAASVLLAVGFAGASVVTSGMPSASALESLARTARAQAA
                     TAIPAGQFAHLVTTSTQTGFQVSAPDPVKAAAGDLPGDPARTETRVLESWTSADGITW
                     RHDTESMSSAAAAGATTTRDWRFCSSGQFSPSSVAALPTTASALEASVRASAQGSNSA
                     DEAVFVALSDLLRLGYADPALRAAGIDVLAGIPGVTTSTVTRNGQPALVVVFRDEAHR
                     PGVAQTLTFDPGTSVLVAEGMTAPDTAYSAVHSQQQFTAEVPAAVVANAENACDTATT
                     KP"
BASE COUNT          505 a         1323 c         1009 g          462 t
ORIGIN      
        1 tcggtcggct tcccgctcac cgagggcgtc agcgacgtcg cgtggccgtg cagcgacacc
       61 aagtacatcg cccacttccc cgagacccgc gagatctggt cctacggctc gggctacggc
      121 ggcaacgccc tgctgggcaa gaagtgctac gccctgcgca tcgcctcggc catggcccgc
      181 gacgagggct ggatggccga gcacatgctc atcctcaagc tcacctcccc caaggggaag
      241 gtccactaca tctgtggcgc gttcccgagc cagtgcggca agaccaacct ggcgatgctc
      301 gagcccacca tccccgggtg gaaggccgag atgattggtg acgacatctc ctggatgcgc
      361 ttcggtcccg acggtcgcct gtatgccgtg aaccccgagt tcggtctctt cggcgtcgcc
      421 cccggcaccg gcatgagcac caacccgatg gcgatgcgca ccatcgaaga gggcaactcg
      481 atcttcacca acgtcgcccg caccgacgac ggcgacgtgt ggtgggaagg catctccaag
      541 gagaagccgg cgcacctcat cgactggaag aaccgcgact ggacgcccga caacggcgag
      601 ctctcctcgc acccgaacag ccggttcacg acgcctgcca agcagtgccc gatgatcgcg
      661 gccgagtacg acaaccccga cggtgtcccg atctcggcga tcctcttcgg tggccgtcgc
      721 gccagcaccg tcccgttggt ctacgaatcg cgcaactgga cccacggcac gttcgtcggc
      781 gccacgctct cctcggagac gaccgccgcc gccaccggtg cggtcggtgt cgtgcgccgc
      841 gacccgatgg ccatgctgcc cttcatcggc taccacgccg gtgactacat gcagcactgg
      901 atcaacatcg gcaagcaggc cgacgagtcc aagctcccga agatctacca ggtcaactgg
      961 ttccgcaagg acaaggacgg ccactggctg tggcccggat tcggcgacaa cagccgcgtc
     1021 ctcaagtggg tcgtcgagcg cctcgaaggc gaagccgacg cggtcgaaac ccccatcggt
     1081 ctcgttcccg ccgccggcgc catcgacacc gagggccttg acatggctcc cgacgacatc
     1141 gacaaggccg tcgccgtgga cctcgacgag tggcgcgccg agctgccgct catcgaggag
     1201 tggttcgcca agatcggtga ccggctgccc gcgcagctgc acgccgagct cgacggcctc
     1261 aaggagcgcc tggcctgatc ggcctgcgtg atcagcatca accctgacga gggccccggc
     1321 attcatgccg gggccctcgt tcgttgtgcg acgctcgttc cgtgcgccac gcagcggggg
     1381 accgaaacgg ccgtgtgaca tccggccccg agcggacatt cctaggtgtg aacccaccac
     1441 cgcccaccac cggcgacgca gacgcaggta tgccgcccgc ccccgccgga tcccagccgg
     1501 gtcgaggtat tccaccatcg cctgacgatg gccgaatacc tcgaccgggc ggcgcccccg
     1561 cccccggcag cgccgacgcg gcgttcgagt cgctctaccg cacctgcgcc ccgcgcgtct
     1621 tcgcctacgt ccgccgccac gtcccgccgg ccgattgcga cgacgtcgtt gccgaggtgt
     1681 tcgtcgtcgc ctggcgccga cgcctcaccg tcccacccga cccgctcccc tggctgatcg
     1741 tcgtcgcccg caacgtcttg cgcggccgcc gccggttggc cgtcagagcc gacaacgtct
     1801 ggtgggaggc cgtgcgcagc cttgccgcac cggcatactc cgcctctccg gaagcctcga
     1861 tcgacgaacg cgaggcgctc ctggaaggcc tggcccgctg tagccgggcc gagcgcgaag
     1921 ccctcctgct cgtcgcctgg gacggcctca ccgccgccca agccgccgac gtcgccggct
     1981 gctcgacgcg cgccttcacc gtgcggctct cccgcgcccg cgcccggctc aacggagccc
     2041 tcggcgagcg cccgcaccgg gccgctcccc tgctactcac cccgtcgacc cctctcgccc
     2101 aggagcactg acatgacgac caccgactcc ctcacccgcc tcgccaccat ccgccccgac
     2161 gaggacaccc tcgcccacga ctggtccccc gcccgccgcg ccgacgtgct cgcccgcgtc
     2221 cacgccgcca cctccgacgc cccggccgcc gccacggtca cccccctgac gcgccgtcgg
     2281 ctcttcccgg cccgccgcct cgtcgcagcc agcgtgttgc tggccgtcgg cttcgctggc
     2341 gccagcgtcg tcacctcagg tatgccgtcc gcctccgccc tggagtccct cgcccggacc
     2401 gcgcgggccc aggccgcgac ggcgatcccg gccggacagt tcgcgcatct cgtgacgacc
     2461 tcgacccaga ccggtttcca ggtgtccgcg ccggaccccg tcaaggcggc cgccggtgac
     2521 ctgccgggcg atcccgcgcg cacagagacc cgggtcctcg agagttggac ctccgccgac
     2581 gggatcacct ggcggcacga caccgagtcg atgagctcgg cagccgcggc gggcgcgacg
     2641 acgacgcggg actggaggtt ctgctcctcg ggccagttct caccgtcgag cgtcgcggcc
     2701 ctgccgacca ccgcgagcgc cctcgaagcg tccgtgcggg cctcggccca gggctcgaac
     2761 tcggccgacg aggcggtgtt cgtcgcgctc agtgacctgc tgcgcctggg ttacgccgac
     2821 ccggccttgc gcgctgctgg gatcgacgtc ctcgctggca tccccggcgt gaccacctcg
     2881 acggtgaccc gcaacggcca acccgcgctc gtcgtcgtct tccgcgacga ggcccaccgg
     2941 ccgggggtgg cccagacgct caccttcgac ccgggcacga gtgtccttgt cgcagaaggg
     3001 atgacggcgc cggacacggc atactccgcc gtccactccc agcagcagtt caccgccgag
     3061 gtgcctgctg cggtcgtcgc caacgccgag aacgcctgcg acaccgccac caccaaaccc
     3121 tgaccccatc accaaaccct gaccccaccc caccccctga tccccgccgg gtcgaggtat
     3181 tccaccatcg cctgacgatg gccacatacc tcgacccggc tgtgcgggtg cagaatccgc
     3241 agccaaaacc aggtgacgcg gcccgcgacc gagggtcacc ctggaaggca tgaccctgg
//
LOCUS       DAOMZY010000373         3275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_28368_length_3275_cov_5.515839, whole genome shotgun sequence.
ACCESSION   DAOMZY010000373 DAOMZY010000000
VERSION     DAOMZY010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28368_length_3275_cov_5.515839"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="PLK64_15555"
     CDS             complement(<1..930)
                     /locus_tag="PLK64_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPV81210.1"
                     /translation="MKKRTLGQGLEVSAIGLGCMGMSSSFPPFPDRADMVVLIRQAVD
                     AGVTFFDTAQVYGLFTNEDLVGEALEPVRDQVVIATKFGFELSTGQPTGVDSRPATIR
                     SSVDDSLRRLRTDTIDLLYQHRVDPNVPMEDVAGTVGELIAAGKVRHFGLSEAGAANI
                     RRAHAVTPVTAHQSEYSLWWREPEHTILPLLRELGIGLVPFSPLGKGFLTGTIDEDTT
                     FGEGDFRNTVPRFADVEARRANQTFVDLIARIAQRRGVTPAQVALAWLLAQGRQIVPI
                     PGTTKLSRVTENLGARKVVLTPADLALIDKADFQ"
     gene            1360..1629
                     /locus_tag="PLK64_15560"
     CDS             1360..1629
                     /locus_tag="PLK64_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013490926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV81211.1"
                     /translation="MLTTVRDLGAAVRHRRREARLTQAMLAQRAGVSRQWLSGFESGK
                     NPAVELGKVLDVLTALDLALELVSAPPPETGTDVDPFAGFFAERT"
     gene            1626..>1800
                     /locus_tag="PLK64_15565"
     CDS             1626..>1800
                     /locus_tag="PLK64_15565"
                     /inference="COORDINATES: protein motif:HMM:NF025041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HPV81212.1"
                     /translation="MTDHLNVYLDGVLAGRLTQTSGGALSFSYDAAYVTRRVPTPLSL
                     SMPLSGSRHSNRVV"
     assembly_gap    1801..1900
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1916..3082
                     /locus_tag="PLK64_15570"
     CDS             1916..3082
                     /locus_tag="PLK64_15570"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HPV81213.1"
                     /translation="MPLSGSRHSNRVVAAWLEGLLPDNLGVREEWGRRFEVSPRNPFA
                     LLRHVGRDAAGAVQVLPADVDPPDAAQRTGKVRRLSHREVNDLLVGLTVRDAGWEVGA
                     GAGRWSLAGVQNKVALHRLDDGDWGVPEDSTPTTHILKPTRAETRFADLHVNEFVCLR
                     AAGHLGLRTAHVELVDFGGAKTLVSTRYDRGRGPDGAWLRLHQEDLLQAMSYPPSKKY
                     QSDGGPSVKNVAALFATLALADRDVVRSSFFDALAFNVLVGGTDAHAKNYSLLLRGSR
                     VALAPLYDVASYAPYLSKGEAARSSMKMGRTWQVRDLTAEDWADVGISLGLDPDDALS
                     RIERLRVGLPEALAAAADEAPEPFAGGARRVADAIARQGHLKARHLPGATRRRG"
BASE COUNT          491 a         1081 c         1085 g          518 t
ORIGIN      
        1 ctggaagtcg gccttgtcga tgagggcgag gtcggcgggg gtgagaacga ccttgcgcgc
       61 gccgaggttc tcggtgacgc gggagagttt cgtggtgccg gggatcggca cgatctggcg
      121 gccctgagcc agcagccagg ccagggcgac ctgggccgga gtgacgccgc gacgttgggc
      181 gatgcgcgcg atgaggtcga cgaaggtctg gttggcgcgg cgtgcctcga cgtccgcgaa
      241 ccgcggcacg gtgttgcgga agtcgccctc accgaacgtc gtgtcctcat cgatggtgcc
      301 ggtgaggaag cctttgccga gcgggctgaa cggcaccagg ccgatgccca gctcgcgcag
      361 cagcggcagg atcgtgtgct cgggctcgcg ccaccagagc gagtattcgc tctggtgagc
      421 cgtcaccggc gtgaccgcgt gcgcgcgacg gatgttggcg gcgcccgcct cggagagccc
      481 gaagtgacgc accttgcccg cggcgatgag ctcgccgacc gttccggcga cgtcctccat
      541 cggcacgttg gggtcgacgc ggtgctggta gagcaggtcg atcgtgtcgg tgcgcagccg
      601 gcggagcgag tcatcgacgc tgctgcgaat cgttgcggga cggctgtcga cgcccgtcgg
      661 ctggccggtg gagagctcga agccgaactt cgtggcgatg acaacctggt cgcgcaccgg
      721 ctcaagcgcc tcgccgacaa ggtcctcgtt ggtgaagagg ccatagacct gcgccgtgtc
      781 gaagaacgtc accccggcgt cgacggcctg ccggatgagg acgaccatgt cggcgcggtc
      841 cgggaagggc gggaagctcg agctcatccc catgcacccc agcccgatcg cggagacctc
      901 caagccttgg ccgagggttc gcttcttcat ctcgtcgtct ccatgtctgt gcatgaccgc
      961 gtggcccacg gacggacgag gacagctatg gcctcagggg gttgtcctgc cacggtaacc
     1021 gaccgcaggc acccccgacg ccgatgaggt atgccgcgca tccccgcccc cgacacgaag
     1081 ccctcccgga ctgcgctcat gaaaggcgag tgcgacccca tctgagccag ccccccgggg
     1141 atgaagccgc gcatgaagtc gccgtagcgt gcgtgatcac cccacctgcc acggccccat
     1201 gaacggcttc tcgcccgcag cctcccgcgg cacgcgccgt agcgatacgg ccgcgtaccg
     1261 gattgtcgtc taacggcgac attttcggat gtcgtggtac tctgacattt ctagatgtcg
     1321 ttcagccgcg acaaatccac gcacgcgacg gagggcagga tgctcaccac ggtccgagac
     1381 ctcggggcgg cagttcggca ccggcgccgc gaggctcgcc tcacgcaagc gatgctggcg
     1441 cagcgggctg gggtctctcg ccagtggctc tccggcttcg agtcgggcaa gaacccggcc
     1501 gtcgagttgg gcaaggtcct ggacgtcctc acggcactcg accttgccct cgaactggtc
     1561 tcggcaccgc ccccagagac agggacagac gtggacccct tcgccggctt cttcgcggag
     1621 cgcacatgac ggaccacctc aacgtctacc tcgacggagt cctcgccggt cgcctgaccc
     1681 agacctcggg tggggcgctg agcttcagct acgacgcggc atacgtgacg cgtcgcgtgc
     1741 ccaccccgct ctccctgtcg atgccactca gtggctcgcg gcacagcaac cgcgtcgtcg
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccgctctccc tgtcgatgcc
     1921 actcagtggc tcgcggcaca gcaaccgcgt cgtcgcggca tggcttgagg gcctgctccc
     1981 cgacaacctc ggcgtccgag aggagtgggg caggaggttc gaagtttcgc cacgaaaccc
     2041 gttcgccctc cttcgccacg tggggcgcga tgccgcagga gccgttcagg ttctccccgc
     2101 tgatgtcgac ccacctgatg cagcgcagcg aaccgggaaa gtccgtcggc tctcccaccg
     2161 cgaggtcaac gatcttctcg tagggctgac cgtccgcgat gccggctggg aagtgggggc
     2221 aggcgcgggc cgctggagcc tcgccggcgt ccagaacaag gtggcgctgc atcgcctcga
     2281 tgatggtgac tggggcgtcc ctgaggactc gaccccgacg acccacatcc tcaagccaac
     2341 gcgtgcggag acgcgcttcg cggatctgca cgtgaacgag ttcgtctgcc ttcgggcggc
     2401 cggccacctc gggcttcgaa ccgcccacgt cgagttggtc gacttcggtg gagcgaagac
     2461 gctggtctcc acccggtacg accggggccg tggtccggac ggcgcgtggc ttcgtctgca
     2521 tcaggaagac ctgctgcagg cgatgagcta cccgccctcg aagaagtacc agtcggatgg
     2581 gggccccagc gtcaagaacg tcgccgccct cttcgccacg ttggcgctcg ccgaccgcga
     2641 tgtcgtccga tccagcttct tcgacgccct cgcgttcaac gtcctggtcg gcggaaccga
     2701 cgcccacgcg aagaactact ccctcctgct gcgcgggtct cgggtcgccc tcgcacccct
     2761 ctacgacgtt gcgtcatacg ccccgtatct cagcaaaggt gaagctgcgc ggagctctat
     2821 gaagatgggg cgcacctggc aggttcgcga cctgacggcc gaggactggg ccgacgtcgg
     2881 gatctccctc gggctcgatc ccgacgacgc tctgtcccgt attgagcgtc tgagagtggg
     2941 gctgcctgag gccctcgctg ccgccgctga cgaagcgccc gagcctttcg ccgggggcgc
     3001 gcgtcgagtc gcggacgcga tagcccgtca gggtcacctg aaagcccgac acctccctgg
     3061 agcaactcgg cggcgcggct aggcgatcgc ggatcccctc cggccgaggc tggctgcggt
     3121 gattcggcct ggaccccagg gtcatcgcgt gtagggaagg gtgtcgtgtc cggtcgcagc
     3181 catgaggtcc tcgtaccacg cgaccaagct gcgggccttg ggcccgtcgg cgtgcaggtg
     3241 aagggtgtcg ccggcgatga gaagttgtgg gtggc
//
LOCUS       DAOMZY010000374         3275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_28374_length_3275_cov_4.067391, whole genome shotgun sequence.
ACCESSION   DAOMZY010000374 DAOMZY010000000
VERSION     DAOMZY010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28374_length_3275_cov_4.067391"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PLK64_15575"
     CDS             complement(<1..259)
                     /locus_tag="PLK64_15575"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPV81214.1"
                     /translation="MPDERVTVFVRGHVQGVGFRWWARARALELGLVGYARNLDDGRV
                     EVLAQGPTEAIDAMVRLLSEQPSSLRRPGRVAGVVAQYAQPI"
     gene            complement(281..2212)
                     /locus_tag="PLK64_15580"
     CDS             complement(281..2212)
                     /locus_tag="PLK64_15580"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPV81215.1"
                     /translation="MTAAQASAPLALPTAASAHDWLSDRAATGLAKARSLVADLKAAP
                     PADALATLKAWNDVDQALGSVSSPASLFAEVHPDEAVRERADVAGQEVQKLATELGLD
                     RALFEVFAALDPAGLDADAARFLNKTLRDFRRSGVDRDEPTRERIRAINERLTVLGQE
                     FSKNIRNGVRSIEVTPDRLEGMPQDWLDAHPADERGLVTVTTDYPDVVPLRTFCADAQ
                     ARRDLTMEFLTVGWPANDALLHELFDLRHELAQLLGYETWADYDAEVKMIKEGGAIPV
                     FIEKIADAALESGVRDREVVLARMRADVPDATAIDSADLAYYANLVSKEQLEVDAQLV
                     RTYLDFTKVRAGLLEVTGRLFGLRYEPAADAVVWDEAVAAYDVYRADGDEPLGRIYLD
                     LHPREGKYKHAAQFDLVTGVAGRQLPEGVLVCNFSRGLMEHDHVVTLFHEFGHLVHHV
                     LAGHQEWFRFSGVATEWDFVEAPSQMLEEWAWDAEILQTFATNEAGEPIPADLVGRMR
                     RADDFGKGYLARTQMFYAALSYWFHTERPADLTARTRELQAQYSLFPYIEGTHFTCAF
                     GHLEGYSSAYYTYMWSLVIAKDLFSAFDRSNMFDAEVAGRYRDRVLAPGGSKDAADLV
                     ADFLGRPYTFDAYAAWLAE"
     gene            complement(2569..>3275)
                     /locus_tag="PLK64_15585"
     CDS             complement(2569..>3275)
                     /locus_tag="PLK64_15585"
                     /inference="COORDINATES: protein motif:HMM:NF025748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK motif protein"
                     /protein_id="HPV81216.1"
                     /translation="ILELAIKRLELSEAALLGLAGELLLLEALVRRTDDAFVGQLVAA
                     WDGWRRSARDLTWDGTGVEVKTTTRPTSSHIVQGTDQVEPALGDDTTPGEDRLLLLSV
                     GLQLSTPSQNSFTVPQLTGRIAERLEATGNSGQVNDFLAHVAEYGAESGLGYRHATMA
                     ADAPFQAEFTPAFVRGYDMGDPAVQVIRRNDVVTHQHVDAGSLSFRIDLPATVSLGNP
                     VDGLQRIADVILGEQP"
BASE COUNT          506 a         1047 c         1206 g          516 t
ORIGIN      
        1 cgatgggctg ggcgtattgc gcgaccacac cagcgacccg accggggcgg cggagggacg
       61 acggttgctc ggacagcaag cgcaccatcg cgtcgatggc ctcggttggc ccctgggcga
      121 gcacttcgac ccggccgtca tcgaggttgc gggcgtagcc gaccagcccg agttccaacg
      181 cgcgggcgcg cgcccaccag cggaacccga ccccttggac gtgcccccgc acgaaaaccg
      241 tcacccgctc gtcaggcatg actgctcacg tgcctggctg ctactccgcc agccaggcgg
      301 catacgcgtc gaaggtgtag gggcgaccga ggaagtcggc gaccaggtcg gcggcgtcct
      361 tgctgccccc cggggcgagc acgcggtcgc ggtagcggcc ggccacctcc gcgtcgaaca
      421 tgttcgagcg gtcgaaggcc gagaacaggt ccttggcgat gaccaggctc cacatatagg
      481 tgtagtaggc gctggagtag ccctccaggt gcccgaacgc gcaggtgaag tgagtgccct
      541 cgatgtaggg gaacagcgag tactgcgcct gcaactcacg cgtgcgcgcg gtgaggtcgg
      601 ccgggcgttc ggtgtggaac cagtaggaca gcgcggcata gaacatctgg gtgcgcgcga
      661 ggtagccctt gccgaagtcg tcggcccgac gcatccgccc gacgaggtcg gctgggatcg
      721 gctcgccggc ctcgttggtg gcgaaggtct gcagaatctc ggcgtcccag gcccattcct
      781 cgagcatctg gctgggggcc tctacgaagt cccactcggt cgcgaccccg gagaagcgga
      841 accactcctg gtgcccggcg aggacgtggt gcacgaggtg gccgaactcg tggaagagcg
      901 tcacgacgtg gtcgtgctcc atcagcccgc gggagaagtt gcagacgaga acgccctcgg
      961 gcagctggcg gcccgcgacc ccggtgacga ggtcgaactg cgcggcgtgc ttgtactttc
     1021 cctcgcgcgg gtgcaggtcg aggtagatcc gcccgagcgg ctcgtccccg tcggcgcgat
     1081 agacgtcgta tgccgcgacc gcctcgtccc agacgacggc gtcggcggcc ggctcgtagc
     1141 gcaggccgaa gaggcgaccg gtcacctcca agagaccggc ccggaccttg gtgaagtcga
     1201 ggtaggtccg caccaactgg gcgtcgacct ccaactgctc cttgctgacg aggttggcgt
     1261 agtaggcgag gtcggccgag tcgatcgcgg tggcgtccgg gacgtcggcg cgcatccggg
     1321 ccaggacgac ctctcggtca cgcacaccgg actcgagggc ggcgtcggcg atcttctcga
     1381 tgaagacggg gatcgccccg ccctctttga tcatcttgac ctcagcgtcg tagtcggccc
     1441 acgtctcgta gccgagcaac tgggccaact cgtggcgcag gtcgaacagc tcgtgcagca
     1501 gtgcgtcgtt ggccggccag ccgacggtga ggaactccat cgtcaggtca cgtcgggctt
     1561 gggcgtcggc gcagaacgtg cgcagcggca ccacgtcggg gtagtcggtc gtcaccgtga
     1621 ccaggccccg ctcgtcggcg gggtgggcgt cgagccagtc ctgcggcata ccctccaacc
     1681 ggtcaggggt gacctcgatc gagcggaccc cgttgcggat gttcttggag aactcctggc
     1741 cgagcaccgt gagccgctcg ttgatcgcgc ggatccgctc gcgagtcggc tcgtcgcggt
     1801 cgacaccgga gcggcggaag tcgcgcagcg tcttgttgag gaaccgggcc gcgtcggcgt
     1861 ccaggccagc cgggtcgagc gcggcgaaga cctcgaacaa cgcgcggtcc aggccgagct
     1921 cggtggcgag cttctggacc tcctggccgg ccacgtcggc ccgctcgcga accgcctcat
     1981 cggggtggac ctcggcgaag agggacgcag gtgaggagac cgaccccagg gcctggtcga
     2041 cgtcgttcca tgccttcaag gtcgccagtg cgtctgcggg cggggccgcc ttgaggtcag
     2101 cgacgaggct gcgggccttc gcgaggccgg ttgcggcccg atcggagagc cagtcgtggg
     2161 ccgaagcggc ggtgggcagg gcaaggggcg cggatgcctg agcagcggtc accgctggag
     2221 cctatgtcac cgcaccgacg tcgcacgtgg tctctgccca ccatggtgtt cgcgggcgtc
     2281 cggctcgcgc gtatgccgcc agcgaccggt cgggtgcaca cccgccacgg tcacagagtt
     2341 cattgcgatt ggcaacgagg cgagtttctc gcgttgcggc tgcggctgat gagcttggcg
     2401 tcgcaggtct agaagggggc ctgccggagt tcacacagga cgaatcttcg agcaggtggc
     2461 caaggcggtt cgatccctgg ggtgaggcga ggtgctccgc agtcatggcg ggtcctttcg
     2521 tggcgaatct gccggggtcc ccggtgcctc ggcggcctcg cccgggagct acggctgctc
     2581 gcccagaata acgtcggcga tgcgttggag gccgtctacg gggttgccga ggctgacggt
     2641 cgccggcagg tcaatgcgga aggacagcga ccctgcgtcg acgtgctggt gggtgacgac
     2701 atcgttccgg cggatcacct gaacggcggg atcgcccatg tcgtatcccc ggacgaaggc
     2761 gggggtgaac tctgcctgga agggtgcgtc cgcggccata gtggcgtgac ggtagccgag
     2821 gccggactcg gcgccgtact cggcgacgtg ggccagaaag tcgttgacct gaccggaatt
     2881 gccggtggct tctagtcgct cggcaatgcg cccggtgagt tgcggcacag tgaaggagtt
     2941 ctggctcggg gtcgagagtt ggagtccaac gctcaacagc aggagacggt cctcgcctgg
     3001 cgtggtgtcg tcacccagcg cgggttccac ctggtcggtg ccctgcacaa tgtggctgga
     3061 cgtggggcgc gtcgtggtct tgacctcgac gccggtgccg tcccaggtga ggtcgcgcgc
     3121 cgaacgccgc cacccgtccc aagcggcgac gagttggccg acgaatgcgt cgtcggtgcg
     3181 acgcacaagg gcttccagca ggaggagttc gcccgccagc ccgaggaggg ctgcctcgga
     3241 gagttccagc cgcttgatgg cgagctcgag gatgg
//
LOCUS       DAOMZY010000375         3256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_28639_length_3256_cov_6.031240, whole genome shotgun sequence.
ACCESSION   DAOMZY010000375 DAOMZY010000000
VERSION     DAOMZY010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28639_length_3256_cov_6.031240"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PLK64_15590"
     CDS             complement(<1..604)
                     /locus_tag="PLK64_15590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPV81217.1"
                     /translation="MSDFLCPASAQYLAAQRWALGGVDRWACIRTGDIAWLHEHFLVP
                     SARTSFIHFPSAGSTSDFGDPLPFALPASFVYASGQAHRDWDTLVAALRIAPVAAVIS
                     VPDAVANGVAATLPDRVTVLPLVAPHHGRALALRASVVVVALKDTHLPAGPLVLVDAL
                     AAGAAIVATDANGSRDYVVAGSTASVVPAGDAPALARAIQD"
     gene            complement(885..1577)
                     /locus_tag="PLK64_15595"
     CDS             complement(885..1577)
                     /locus_tag="PLK64_15595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HPV81218.1"
                     /translation="MFGIAGLAVEPTYHGLAAHRERLIRTHSIGHVLDVGANTGQYAK
                     ALLASGYQGLVTSFEPNPRAAKELRATAAGRPAWTVHEVALTSSEGRMTLHVTDDSQS
                     TSLLVPTADYAFMQKNHEVEVDTQTLDAFGPFASPADAKGLLLKMDVQGFEGDVLRGA
                     ARTLESCSVVECELSTVELYAGQALMEEVIAQLRRAGLFPVALVRGFTDPTSHNVIQY
                     DGIFARSGSTRG"
     gene            complement(1694..2674)
                     /locus_tag="PLK64_15600"
     CDS             complement(1694..2674)
                     /locus_tag="PLK64_15600"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HPV81219.1"
                     /translation="MSTAPVKVAVTGAAGQIGYSLLFRIASGALLGPDTPVQLRLLEI
                     TPALKALEGVVMELDDCAFPTLAGVEIGDDPEKVFEGTNLAMLVGAMPRKEGMDRADL
                     LAANGKIFTGQGAALNKVAADDVKILVTGNPANTNALIASANAPDIPKERFNALTRLD
                     HNRAKTQLAQKLGVSVEAITQLAIWGNHDDSMYPDLYNTKVNGTTAADLVDEAWITDT
                     FIPTVATRGGAIIKARGLSSAASAANATIDHMRDWALGTSDWVSMSVPSDGSYGLTEG
                     LISSFPCVCVNGGYEIVQGIELSDFSLERIKASVARLESERDQVKALGLI"
     gene            complement(2839..>3256)
                     /locus_tag="PLK64_15605"
     CDS             complement(2839..>3256)
                     /locus_tag="PLK64_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81220.1"
                     /translation="GGRGGRDSGGRGGRGGRDSGRDSGRDGGRDGSREGAVRNGTDGG
                     EASPAASEGEAPRRQRNRRRTRAGEEGSAAPREGAAAPDASSNGSGRSAASAAGDASN
                     LREGGETRRPRRRRRRGSGQGGGDATATAAATSAAE"
BASE COUNT          522 a         1077 c         1137 g          520 t
ORIGIN      
        1 ggtcttggat ggcccgtgcg agagccggcg catccccggc tgggactaca gacgcggtgg
       61 aacctgcgac aacgtagtcc cggctgccgt tggcgtctgt cgcgacgatg gcggctcctg
      121 ccgcgagggc atcgacgagg accagggggc cagccggaag atgggtgtcc ttcagcgcga
      181 caacgacgac cgaggcacgc agcgccagcg ctcgaccgtg gtggggtgca accagcggaa
      241 ggacggtcac ccgatcaggc aaggtcgcag caacaccgtt ggctacggcg tcgggaacgc
      301 tgatcacggc ggcgacaggg gcgatcctca gggccgcgac gagggtgtcc cagtcgcgat
      361 gggcttgccc gctggcgtag acgaaggagg cgggcagcgc aaacggaagt ggatctccaa
      421 agtcggatgt gctgcccgca gagggaaagt ggatgaatga cgttcgcgcg ctcggcacca
      481 ggaagtgctc atgcagccac gcgatgtcgc cggttcgaat gcaggcccaa cggtcgactc
      541 cacctagcgc ccaccgttgc gctgccaggt actgggcact ggctgggcac aggaaatcac
      601 tcacgacaag gtggtcgggc ccaagccgcg gcgcgagcct tcgcatcgcc cctgccacga
      661 aggcttgcgc gcccacggtg tgcaacacaa gcccttggga tcgccgcgcg gcccacatga
      721 cgcgcaggac gtccatgctc aggtgaacca gtcgagttcg ccgcccgact ccccagtctg
      781 atcgcatgcg gaccacacga acgtctcccg cagcggcctc gaaagctgag accacgacat
      841 cgggcgtggt ccacgccaca gccacgatga tgccgtcgtg gctctcagcc acgtgtggat
      901 ccggaccgcg cgaagatgcc gtcgtattgg atgacgttgt gtgaagttgg gtcggtgaag
      961 ccccgcacga gtgccaccgg gaagagcccg gctcgtcgga gctgcgcgat cacctcttcc
     1021 atgagggctt gacccgcata gagctcgacc gtggacaatt cacactcaac gacgctgcag
     1081 ctttccaggg tgcgtgctgc gcccctcaaa acgtcgcctt cgaaaccctg aacatccatc
     1141 ttgaggagga gtcccttcgc gtcagcgggc gacgcgaagg gaccgaatgc atcgagggtt
     1201 tgagtatcga cctcgacctc gtggttcttt tgcatgaagg cgtagtccgc ggtgggcacc
     1261 aacaacgacg tactctgcga gtcatccgta acgtgcaggg tcatcctgcc ctcgctcgag
     1321 gtgagcgcca cctcatgcac cgtccaggca gggcgaccgg ccgctgtcgc tcgcaactcc
     1381 ttagccgcac ggggattggg ctcaaatgag gtaaccagcc cttgataccc actcgccagc
     1441 agcgccttcg cgtactgccc ggtgttcgcg ccgacgtcaa ggacatggcc gatcgagtgc
     1501 gtgcgaataa gccgctcccg gtgcgcagcc aagccgtggt aggtcggctc gaccgcgagc
     1561 ccggcgatcc caaacacgtc tgcggcgagg cgacgtcgag cgtcacgatc accgagaagt
     1621 gctcgagaca gggtccaatc cgaggctgcc atctccttct gcgactcccg tcgtagtcat
     1681 ggcccaactg gtctcagatg agacccagtg ccttgacctg gtcgcgctcg gactccaagc
     1741 gggccacaga ggccttgatc cgctcgagcg agaagtcgga cagctcgatg ccctggacaa
     1801 tctcgtagcc gccgttgacg cacacgcacg ggaacgagga gatgagcccc tcggtgaggc
     1861 cgtaggagcc gtccgacggc accgacatcg acacccagtc gctggtgccg agggcccagt
     1921 cgcgcatgtg gtcgatcgtc gcgttggccg ccgaggctgc cgacgacagg ccacgggcct
     1981 tgatgatggc gccgccacgg gtggcgaccg tcgggatgaa ggtgtcggtg atccaggcct
     2041 cgtcgacgag gtcggcggcg gtcgtgccgt tgaccttggt gttgtagagg tcggggtaca
     2101 tcgagtcgtc gtggttgccc cagatcgcga gctgggtgat cgcttcgacg ctgacgccga
     2161 gcttctgggc cagctgggtc ttggcccggt tgtggtccag gcgggtcagc gcgttgaagc
     2221 gctccttggg gatgtcgggg gcgttggcgc tggcgatgag cgcattggtg ttggccgggt
     2281 tgcccgtgac gaggatcttg acgtcgtcag cggcgacctt gttgagggcc gcgccctggc
     2341 cggtgaagat cttgccgttg gccgcgagca ggtcggcgcg gtccatgcct tccttgcgcg
     2401 gcatggcgcc gacgagcatg gccaggttgg tgccctcgaa gaccttctcc gggtcgtcac
     2461 cgatctcgac gccggcgagg gtcgggaacg cgcagtcgtc gagctccatg acgacgccct
     2521 cgagtgcctt gagggccggc gtgatctcga gcagacgcag ctgcacgggg gtgtcggggc
     2581 cgagaagggc gccgctggcg atgcggaaca gcaggctgta gccgatctgg ccggcggcgc
     2641 cggtgacggc caccttgacg ggggcggtgc tcacgggatc tccctttgcg cggttggagt
     2701 ggggtcgtcc cgacgctacc agcagccgtc ggcacgcccc tcaaccgcgt ctcaggcagg
     2761 gggtatgccg tggatcacca aagggccgcc ccaccctgag tgggtgggac ggccccattc
     2821 cgcgctagga cggtcggtct actccgcggc ggaagtggcc gcagcggtcg cggtcgcgtc
     2881 gccgccgccc tggcccgatc cgcgccgacg gcgccgacgc ggacgacgag tttcgccgcc
     2941 ctcgcgaagg ttcgacgcgt cgcccgctgc gctggcggcg gaacggcccg atccgttgct
     3001 cgatgcgtcg ggggctgcgg caccctcgcg gggggccgcg gagccctcct cgccggcgcg
     3061 ggtccgacgt cggttgcgct ggcggcgcgg tgcctcaccc tcgctagcgg ccggggacgc
     3121 ctcgccgccg tcagtgccat tgcggacggc accctcgcgg ctgccgtccc gaccaccgtc
     3181 acggccgctg tcacggccgc tgtcacggcc accacgtccg ccgcgcccgc cgctgtcgcg
     3241 accgccacga ccgccg
//
LOCUS       DAOMZY010000376         3248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_28750_length_3248_cov_4.837457, whole genome shotgun sequence.
ACCESSION   DAOMZY010000376 DAOMZY010000000
VERSION     DAOMZY010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28750_length_3248_cov_4.837457"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PLK64_15610"
     CDS             complement(<1..291)
                     /locus_tag="PLK64_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81221.1"
                     /translation="MATVTPALWRRWGPWALGLVGALAALGLQTQLYTGQVRTVATIF
                     MFVALALSWNLIGGYTGYASFGQVVFFGLGGYFAAVTMTHLHWSFWVALPLAG"
     gene            complement(291..1175)
                     /locus_tag="PLK64_15615"
     CDS             complement(291..1175)
                     /locus_tag="PLK64_15615"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPV81222.1"
                     /translation="MTGFLQATVYGLLQGGLLALVAVGFSLVWGVMNVVNLSHGAFVV
                     LGAYVGWQLNTSLGLDPFLGMVIVALVLFCIGYAVQRFLINLVVNGPIFITLLLTFGL
                     ELLLVQGMNIVFTANYQSIPTSYAGNAIVLGGIQVPLGRLLAFLLAVGITVLLVKVMA
                     NTRIGMSIMATGMDRGAARLMGIRARHVYALTFGIATALAGMAGTMVGTVGTFNPAAA
                     GGFTLLSFVIAVLGGLGNMYGAMVGGLLMGLVQAWGGYYLSGTWVNALAFLVLVIVLM
                     FRPSGLVGKAFYAARVEV"
     gene            complement(1172..2404)
                     /locus_tag="PLK64_15620"
     CDS             complement(1172..2404)
                     /locus_tag="PLK64_15620"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HPV81223.1"
                     /translation="MKTSRILATATIAAVSVALTGCGSKGEAGGDAGASSGTLTIGAS
                     ISLTGKTAREGVLTQEGYQLCQEKVNAKGGVKVGDKMLKLDIKYQDDTSKPDTAASLV
                     DQFNDQGIKLILGSYGSANTEAQAAVIERNNQVMVDSSGADNNIFAKGYKRTFGVLSP
                     ATEYAASIVKAVAELASPKPTSVVVLSADDGFSKRAADGAAKAAKDLGFTVLETQYFP
                     NGATDVSASLNKVKASNPDVIIGSVHLAEGVAIIKQAKELGVVPKVFGETVAPPTPDF
                     VTTLGKDAEGVLGSSQWTKSAAGSDDYFGTAKQYDADIQAKFKHEAGYHNAEASAACL
                     AFVLAIQKAGSTDANKVRDALAALDAPSFFAQLKFDAVGMNSTKPMSVIQIQGGKQVT
                     VWPKTSAEAALIWPGTTK"
     gene            complement(2522..3193)
                     /locus_tag="PLK64_15625"
     CDS             complement(2522..3193)
                     /locus_tag="PLK64_15625"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPV81224.1"
                     /translation="MRPDEIASAVRRAVLERVIHPGQVLNQEELAKWFGVSRIPLREA
                     LRTLVGEGLIIIKPGLGAIVTELDLEAVDELYGLRLRLEPPLAADIVEHVQPTDVSEL
                     RRMSDRLVDVMEERGEAWSSTAYAFNRRLYEVSARRHTVRLVVQVLNLVEPYARMHAQ
                     IPGARDHTTEVAGALVRALEKGNAVKVERRIHEGVESTRSVLITAMASVTNVAEQPPG
                     GESGH"
BASE COUNT          569 a         1048 c         1090 g          541 t
ORIGIN      
        1 tcccgcgagg ggcagcgcca cccagaagct ccagtgcagg tgggtcatcg tcaccgcggc
       61 gaagtagccg ccgagcccga agaacaccac ctgtccgaag gaggcgtagc cggtgtagcc
      121 accaatgagg ttccaggaga gcgcgagtgc gacgaacatg aagatcgtcg ccaccgtgcg
      181 gacctggccg gtgtagagct gggtctgcag cccgagggcc gccaacgcac cgaccaagcc
      241 cagggcccac ggcccccagc ggcgccagag ggccggcgtc acggtggcca tcagacctcc
      301 actcgggctg cgtagaacgc cttcccgacc aggccggacg ggcggaacat gaggacgatg
      361 accaggacga ggaaggccaa cgcgttgacc caggtgccgg agaggtagta accgccccag
      421 gcctggacca ggcccatcag cagaccgccc accatcgcgc catacatgtt gcccagtccg
      481 ccgagcacgg cgatgacgaa cgacagcaac gtgaagccgc ccgccgcagc cgggttgaag
      541 gtgccgaccg tgcccaccat ggtgccggcc atcccggcca gcgccgtggc gatcccgaag
      601 gtcagggcgt agacgtgccg cgccctgatc cccatcagcc gcgcggcgcc gcggtccatc
      661 cccgtggcca tgatcgacat accgatccgg gtgttggcca tcaccttcac cagcaggacg
      721 gtgatcccga cggcgaggag gaaggccagg agtcggccga gcggcacctg tatgccgccc
      781 agcacgatcg cgttaccggc atacgaggtc gggatgctct ggtagttcgc ggtgaagacg
      841 atgttcatcc cctgcacgag cagcagttcc aacccaaagg tgagcagcag ggtgatgaag
      901 atcggcccgt tgacgacgag gttgatgagg aagcgctgca cggcataccc gatgcagaac
      961 agcacgaggg cgacgatcac catccccagg aaggggtcaa gccccaagga agtgttgagt
     1021 tgccacccga cataggcgcc gaggacgacg aacgctccgt gcgagaggtt gacgacgttc
     1081 atcacccccc agaccaggga gaagccgacg gccacgaggg cgagcagacc tccctgcaac
     1141 aacccgtaga cggtcgcttg gaggaaaccg gtcacttggt cgttcccggc cagatgagtg
     1201 cggcttcggc ggaggtcttg ggccacacgg tgacctgctt gccgccctgg atctggatga
     1261 cactcatcgg cttggtggag ttcatgccga ccgcgtcgaa cttcagctgg gcgaagaacg
     1321 acggggcgtc gagagcggcc agagcgtcgc gcaccttgtt ggcgtcggtc gagccggcct
     1381 tctggatggc caggacgaag gccaggcacg cggcgctggc ttcggcgttg tggtagcccg
     1441 cctcgtgctt gaacttggct tggatgtcgg cgtcgtactg cttagcggtg ccgaagtagt
     1501 catccgagcc ggcggccgac ttggtccact ggctcgaccc gaggactccc tcagcgtcct
     1561 tccccagggt ggtgacgaag tcgggcgtgg gcggtgcgac ggtctcgccg aacaccttcg
     1621 gcaccacacc gagttccttg gcctgcttga tgatggcgac gccttcggcc aggtgcaccg
     1681 agccgatgat gacgtccggg ttcgacgcct tcaccttgtt gagcgacgcg ctcacgtcgg
     1741 tggcgccatt ggggaagtac tgggtctcca gcacggtgaa cccgaggtcc ttggcggcct
     1801 tggcggcgcc gtcagcggca cgcttggaga agccgtcgtc ggccgaaagg accacgaccg
     1861 aggtcggctt ggggctggcc agttcggcga ccgccttgac gatcgacgcg gcatactccg
     1921 tggccggcga gagcaccccg aaggtgcgct tgtagccctt ggcgaagatg ttgttgtcgg
     1981 cgccgctgga gtcgaccatg acctggttgt tgcgctcgat gaccgcagcc tgagcctcgg
     2041 tgttggccga gccgtaggac ccgaggatga gcttgatgcc ctggtcgttg aactggtcga
     2101 cgagcgatgc ggcggtgtcg ggcttggacg tgtcgtcctg atacttgatg tccagcttga
     2161 gcatcttgtc gccgaccttg acgccgccct tggcgttgac cttctcctgg cagagctggt
     2221 agccctcctg ggtcaggacg ccttctcggg ccgtcttgcc ggtcaaggag atcgatgcgc
     2281 cgatcgtcaa ggtccctgag ctggcaccgg cgtcgccgcc tgcttcgccc ttggaaccgc
     2341 acccggtgag cgcgacgctc acggctgcga tcgttgctgt tgcgaggatc cgggaagtct
     2401 tcatcgacat acctttcggc ccgagcgagc agataattca ctgtatacag ggctcgcgaa
     2461 tacaaacagg gaccgttaat tcgatggtcg ggaacagcgc cgaacggcag ttcgctgccg
     2521 gtcagtggcc ggattcgccg ccgggcggtt gttcggcgac gttcgtcacc gacgccattg
     2581 ccgtgatcag gacgctgcgg gtggactcga cgccctcgtg gatccgccgc tcgaccttca
     2641 cggcgttgcc cttctcaagg gctcgcacca gggccccggc cacctcggtc gtgtggtcac
     2701 gggcaccagg gatctgggcg tgcatccggg catagggctc gacgagattg aggacctgaa
     2761 cgaccaaccg cacggtatgc cgcctggccg acacctcgta gagccgtcgg ttgaacgcgt
     2821 aggcggtgct cgaccacgcc tccccgcgct cctccatgac gtcgaccagt cggtcggaca
     2881 tccgccgcaa ttcgctgacg tccgtgggtt gtacgtgctc gacgatgtcg gcggccagcg
     2941 gtggttccaa ccgcaggcgc aggccataga gctcatccac ggcttcgaga tccagctcgg
     3001 tgacgatggc gccgaggccg ggcttgatga tgatcagccc ctcaccgacg agggtgcgca
     3061 gggcctcgcg cagggggatg cggctcacgc cgaaccactt ggccagctct tcctggttga
     3121 gcacctgacc tgggtggatg acgcgttcga gcaccgcgcg gcgcaccgca ctggcgatct
     3181 catccggacg catcgacgcc ttctccgttg gtttccgccg cactggagca tacgacaact
     3241 tggcagcg
//
LOCUS       DAOMZY010000377         3247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_28763_length_3247_cov_5.846805, whole genome shotgun sequence.
ACCESSION   DAOMZY010000377 DAOMZY010000000
VERSION     DAOMZY010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28763_length_3247_cov_5.846805"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..494
                     /locus_tag="PLK64_15630"
     CDS             180..494
                     /locus_tag="PLK64_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPV81225.1"
                     /translation="MVDVVRSSTFDRWLSKLRDRRAAARVLIRINRLAAGNPGDVKPV
                     GGGISELRIDYGPGYRVYYLREGDRLILLLCGGDKSTQSADIAAAHDIAQQWRANQGG
                     DR"
     gene            491..793
                     /locus_tag="PLK64_15635"
     CDS             491..793
                     /locus_tag="PLK64_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007272458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HPV81226.1"
                     /translation="MSDKSETFSPFDAADYLATFDDVAAYLEAVIDEGDDDPVLIAQA
                     LGAIARSGNMSELARRSGMSREGLYKALSADGNPSFATVLKVSRALGLRLHFESIA"
     gene            937..1287
                     /locus_tag="PLK64_15640"
     CDS             937..1287
                     /locus_tag="PLK64_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81227.1"
                     /translation="MADETNWVRGALPLAISPWLVIAGVVPAGLMDEPLLFGLGCIAG
                     GLAIESARRATPGADSSDWLMVFSRGQFAGMGDLTAVRWCPRAALSGLAVLIAMFSFL
                     VVMSVLSFLGGVDE"
     gene            complement(1395..2048)
                     /locus_tag="PLK64_15645"
     CDS             complement(1395..2048)
                     /locus_tag="PLK64_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81228.1"
                     /translation="MYATVDFFDGGTDLGFTALSAVLGLSFTVGPFVPVVRLEEHTVY
                     ARGLVFHRVLALRDIVDVRPGYSGLSIETEDGSVFEATGVGEKWNIARWMGRRTAADS
                     VAELILDAAAVAQQGQGPGAGTRVADSALAVSTDRARSASSDSSKNHARGLVWLNRVV
                     QAISSLAVGAFLVQTVRGVDIPTWVVLLVGLSVLVSVGTQLLVMRASSSTQEPGSSA"
     gene            complement(2406..3020)
                     /locus_tag="PLK64_15650"
     CDS             complement(2406..3020)
                     /locus_tag="PLK64_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81229.1"
                     /translation="MDFFPDPPPPHEEEPEEGYQPVWSSAPEDVLPGVVPVELLIARS
                     ASTVVMLTGIRAFPEGLAMTLAIRLRGPLERRDLHSEVFDGPYQHSMGEQWQAERLKW
                     GFEFSDGRRATNVDPPAWDAEDERPWKDKSWRPDRPVLNGQGGGGGQRAIDRDYWLWP
                     LPPPGRLRVVFQWPQQGIETTTHDVDAQPFLDAAARSQPVWGPE"
BASE COUNT          574 a         1074 c         1059 g          540 t
ORIGIN      
        1 tagagcaaga gccttctaat ctcgaggtcg caggttcgag tcctgccggg ggcgccgtcg
       61 agaacatgtc ctgacctgca agaatacgat tgctcattcc gaagcgtgca ctaagcgtgc
      121 actagtgccg gctcctcgtg cccttgctcg gaacagcatc tgttaactat ggttgacaca
      181 tggtcgacgt cgtcaggtcc tcgacgttcg atcggtggct aagcaaactg agggatcgcc
      241 gggctgccgc ccgggtcctg atccggatca atcggctcgc cgcgggcaac cccggcgacg
      301 ttaaaccagt cggaggcggc atctccgagc tccgcatcga ctacggaccg ggctaccggg
      361 tctactacct gcgcgaggga gaccggctga tcctgttgct ctgcggcggt gacaagtcga
      421 cccagagtgc tgacatcgcg gccgcgcacg acatcgccca acagtggcga gcaaaccaag
      481 gaggagaccg atgagcgaca agagtgagac cttctccccc ttcgacgctg ccgactacct
      541 ggccaccttc gacgacgtcg cggcctacct cgaggccgtg atcgacgaag gcgacgacga
      601 ccccgtcctg atcgcccagg cactgggggc catcgcgcgt tcgggcaaca tgagcgaact
      661 cgcccgccga tccgggatga gccgcgaggg tctctacaag gccctgtccg ccgacggcaa
      721 ccccagcttc gccaccgtcc tcaaggtgtc ccgggccctg ggtctccggc tgcacttcga
      781 gtccatcgcc tgagggaagc gacgccgtcc gcgccagctc accatccgtg gcagatctgg
      841 taaacgcctc acgctcatgg gcggatcccg agggccgctc gcgagcgaac gcaactgcca
      901 gaccgaccgg tcgacgctac tggctagtct cgccccgtgg ccgacgagac gaactgggta
      961 cgaggggctc ttccgctcgc gatctcacca tggctggtca tcgcgggtgt cgtgccagca
     1021 gggctgatgg acgagccact cttgtttggt ctcggatgta tcgcgggcgg tctggcgatc
     1081 gagagcgcac gccgcgcgac acctggagcc gactcgtccg actggttgat ggtgttcagt
     1141 cgagggcaat ttgccgggat gggcgacttg actgcagtgc gatggtgccc aagggcggct
     1201 ctgtcgggac tggcggttct catcgcgatg ttctcgtttc tggtggtgat gtcggttctc
     1261 tcgttcctcg gcggggtcga cgagtgatca cccattgcgg cagttccgga agcatgggtg
     1321 gcctcccgac gccgccaccg tcgcctaagg gttgtggccg tgaggcggcg caatgtgagt
     1381 gcgtgtcggc gaagtcaggc cgacgaccca ggctcttgcg tggatgagct ggcgcgcatg
     1441 acgagcagct gtgtgccgac ggaaaccaag accgacaacc ccacaagcag caccacccat
     1501 gtggggatgt cgacgcctcg gacggtctga actagaaacg ctccgacagc gagactcgag
     1561 atcgcttgga ccacccggtt gagccacacg agccctcggg cgtggttctt ggaagagtcg
     1621 gacgacgcgg aacgggcccg gtcagtcgag accgcaagcg ccgagtccgc cacccgcgtt
     1681 cctgcgcccg ggccctggcc ctgttgggcc actgcagcgg catcgagaat cagctcggcc
     1741 accgagtcgg cggcggtgcg cctacccatc caccgggcga tgttccactt ctcgcccaca
     1801 cccgtggcct cgaagaccga gccgtcctcg gtctcgatgc tcaagcccga ataccccggg
     1861 cgaacatcga caatgtcccg caaagccaac actcgatgaa aaaccaggcc gcgagcgtag
     1921 acggtgtgct cctcaaggcg cacgactggc acaaatggcc caaccgtgaa ggagaggcct
     1981 agcaccgcag acagcgccgt gaaccccagg tccgtccctc cgtcgaagaa gtcaacggtt
     2041 gcgtacaaga aaaacagacc gatcgccggg aagacgattc ggtaccacag aggcgttcgc
     2101 catgatccgg tgcggatgtc gccagacgca cctgcacccc gcgcagcgac tcgccgaaca
     2161 cgtcgggact cccacccctt gactgatccg gaattggtaa cgccgctttc gtccacgccc
     2221 gagatgctac gggtccgctg cccgagttgt gctgtcttgg ccacccgacc atggcccccc
     2281 cgacgggcat ccaggttgcg gcatgaccgg aagcatcttc accgtggcga gacttggcat
     2341 cagcacggac ggcgcgagcg taccgccggt cagctccctg gtcgtccccc aatacggctt
     2401 ggtgcctact ccgggcccca tacgggctgg gagcgtgcgg cggcgtcaag gaacggctgc
     2461 gcgtccacgt cgtgagtggt ggtctcgatg ccctgttgag gccactgaaa gaccacccgc
     2521 aggcgaccgg gcggcggcag gggccacaac cagtagtcac ggtcgatcgc gcgttggccg
     2581 cctccgcctc cttggccatt gagcacggga cgatccgggc gccaggactt gtccttccag
     2641 gggcgttcgt cctcggcgtc ccaagcgggt ggatcgacgt tggtggcccg gcgaccgtca
     2701 gagaattcaa agccccactt caagcgctcg gcctgccact gctcgcccat gctgtgctgg
     2761 taggggccgt caaagacctc agagtgcagg tcccggcgct ccaggggacc acgcagtcgg
     2821 atggccaagg tcatggccaa cccctcgggg aaggcgcgga tgccggtgag catcacgacc
     2881 gtgctcgccg accgggcgat gagcagttcc acgggcacca ccccgggcag gacgtcttcg
     2941 ggggcgctgg accacacggg ctggtagccc tcctcgggct cttcctcgtg cggtgggggc
     3001 gggtcgggga agaagtccat cgggtgccac ctcctgtgct cggccgtcca agtgcttcgt
     3061 gaagtcgtgc tcagcatgtc gtgcttcggc ttcgccgtca cgacattgcc ggtgagcggt
     3121 cgaggggacg gcggtgcgct tcgcgcgtgc ggccgaagcg tgcgacgcgc aaggactgag
     3181 cacgcgcgaa tcgcggcaaa tccggataag ccctcacgct ggtgtgcgga cgaggccgga
     3241 ccctgat
//
LOCUS       DAOMZY010000378         3227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_29029_length_3227_cov_4.986759, whole genome shotgun sequence.
ACCESSION   DAOMZY010000378 DAOMZY010000000
VERSION     DAOMZY010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29029_length_3227_cov_4.986759"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..641)
                     /locus_tag="PLK64_15655"
     CDS             complement(132..641)
                     /locus_tag="PLK64_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HPV81230.1"
                     /translation="MTRASTPDAPVTPVAVQDSYPPDVAVCYGCGRLNDDGLHIRTFW
                     DGTEGTATFQPKPFHTAITGVVYGGLLASLIDCHAVGTAAAARYAADDREVGSEPVLR
                     YVTGLLQVSFKAPTPMGGPLTLRAWPVEVGERKVIVDAEVIADGQVTVTGHVIAVRLR
                     HDSTGSVAP"
     gene            681..1847
                     /locus_tag="PLK64_15660"
     CDS             681..1847
                     /locus_tag="PLK64_15660"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPV81231.1"
                     /translation="MSRPVGNPALAPFGTTIFATMSALALRHGAINLGQGFPDSDGPQ
                     EVLDAAVAAIRGGRNQYPPGPGTPELLAAIAAHQQRFYGIELDPSREILVTVGATEAI
                     AATILALVQPGDEVVTFEPYYDSYAASIALAGGVRRTSVLRFPDFAVDEASLRAAFSA
                     RTRMVLLNTPHNPTGKVFTRAELDLVCALAREHDAWVVTDEVYEHLIFDGVEHIAVST
                     LPGMWDRTLTISSAGKTFSTTGWKVGWVSGPAEAVAAVRAVKQFLTYVGSGPFQPAVA
                     TGLGLPDEVYAGLAGSLARKRDILCDGLAAAGLPVSRPAGTYFVVADAAALGATDALE
                     FCHWLPEAAGVVGVPVSVFHDDPDAARTLVRFAFCKRDEVLTEAVRRLAGLRVG"
     gene            1961..2545
                     /locus_tag="PLK64_15665"
     CDS             1961..2545
                     /locus_tag="PLK64_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPV81232.1"
                     /translation="MQWFASVGLVLALGVGAAAANTPAAVIQPRPPTAGTQGTAVTAV
                     TAVTPGTALAPGTAVSRWVWPIEPNHVVVHGFDPPATRWSSGHRGVDIAAAVGTSVLA
                     PTDGQVSFVGMVAGRPVLVLTHAGGLRSTFEPVSTTAAVGQLVRRGEAVGTVAAYPGH
                     CAPASCLHWGVLRGATYLDPLALLAGRVILLPMR"
     gene            complement(2554..>3227)
                     /locus_tag="PLK64_15670"
     CDS             complement(2554..>3227)
                     /locus_tag="PLK64_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3152 domain-containing protein"
                     /protein_id="HPV81233.1"
                     /translation="AVPPTASTGTASEPAPSESMGEVEEGAPEPTAAPGAALAPVQAS
                     AKGTMHPIAIPDAPWASAGRAVRLSIEVEDGLSIDEALFAATVAGILSDPQGWQTADG
                     VRFVPVSPAQIAAGAGVDVRVTLATPTLTGKLCGPLNVTAANVSCWWGGRAVLNLHRW
                     VRGSATYGTDLTSYRTYLVNHEVGHGLGHRHVSCPAPGRPAPIMVQQTLGLEGCRAWP
                     WPTQQ"
BASE COUNT          443 a         1100 c         1186 g          498 t
ORIGIN      
        1 cgcacgaatc tcgttgtcgc gagcggcgat ccgttcgaga gctcgctcca cggcaaacct
       61 cgcagtatgc cgcccgctgc ggaccgcgtc agccgtctcg accgcggtgg tggcccgaag
      121 ttgggcgtca gtcatggggc aacggagccg gtgctgtcgt ggcgcagccg aacggcgatg
      181 acatggccag tgaccgtgac ctggccgtcg gcgatcacct cggcatcgac gatgaccttg
      241 cgttccccca cctcgaccgg ccaggcccga agcgtgagcg ggccacccat gggggtcgga
      301 gccttgaagc tcacctgcag caggcccgtg acatagcgca gcaccggctc gctgccgacc
      361 tctcggtcat ccgcggcata ccgggcggcg gctgccgtgc cgaccgcgtg gcagtcgatg
      421 agcgacgcga gcagcccgcc atagacgacg ccggtgatgg ccgtgtgaaa cggtttcggc
      481 tggaaggtcg ccgtgccctc ggtgccgtcc cagaaggtcc ggatgtgcag gccgtcgtcg
      541 ttgagccggc cacagccgta acagaccgca acgtccggtg ggtaggagtc ctggaccgcg
      601 acgggtgtta cgggcgcatc aggcgtcgac gcacgagtca tggggacacg ctagccgcgc
      661 gagcggcata cggtgagggc atgagccgtc ccgtcggaaa cccggcactc gcgcccttcg
      721 gcaccacgat cttcgccacg atgtcagcgc tcgcgctgcg acacggggcg atcaacctcg
      781 ggcaggggtt ccccgactcc gacgggccgc aggaggtgct cgacgccgcg gtggcggcga
      841 tccggggcgg gcgcaaccaa tacccgcccg gcccgggaac gcctgagctg ctcgccgcga
      901 ttgcggcaca ccagcagcgg ttctacggga tcgagctgga cccctcgcgg gagattctcg
      961 tgaccgtcgg ggcgaccgag gcgatcgccg cgacgatcct cgcactcgtg cagcccggcg
     1021 acgaggtggt caccttcgag ccctactacg actcgtatgc cgcatcgatc gccctcgccg
     1081 gcggggtgcg gcggacgtcg gtgctgcgct tccccgactt cgcggttgac gaggcgtcgt
     1141 tgcgcgcggc gttctcggca cgcacgcgga tggtgctact caacaccccg cacaacccga
     1201 cggggaaggt cttcacccgc gccgagctcg acctcgtgtg tgccctcgcg cgtgaacacg
     1261 acgcgtgggt ggtgaccgac gaggtctacg agcacctcat cttcgacggc gtcgagcaca
     1321 tcgcggtgtc gacgctgccg gggatgtggg atcggacgct caccatctcg tcggccggca
     1381 agaccttctc gacgaccggg tggaaggtcg ggtgggtgtc cgggccggcc gaggcggtgg
     1441 ccgccgtgcg cgcggtcaaa cagtttctca cctacgtcgg atccgggccg ttccagcccg
     1501 ccgtcgccac cgggctcggt ctgcccgacg aggtgtatgc cggtctcgcc ggctccctgg
     1561 cgcgcaagcg cgacatcctc tgcgacgggt tggctgctgc cgggctgccc gtgtcgcggc
     1621 cggccgggac ctacttcgtc gtggctgatg ctgcggcgtt gggcgccacc gatgcgttgg
     1681 agttctgtca ctggctgccc gaagccgccg gggtggtcgg ggtgccggta tcggtcttcc
     1741 acgacgaccc cgacgcggct cggaccttgg tgcgtttcgc cttctgcaag cgcgacgagg
     1801 ttctcaccga ggcggtgcgg cgactggctg gcctccgggt cggctgagcc cgggcctcag
     1861 ggcgcccggt ttatccacac cccgtcgtcg ggttgtcccg tcatccacac gctgggagtg
     1921 acggttggac gggcccgacc ggatgtcgca gggtcgaggc atgcaatggt tcgcatcggt
     1981 cggcctggtg ctggcgttgg gggtgggtgc cgccgcggcg aacacgcccg cggcggtgat
     2041 ccagccgcgt ccgcccaccg cgggcacgca ggggaccgcg gtgaccgccg tgactgcggt
     2101 gacgccgggg acggccctgg cgccggggac tgcggtgagc cggtgggtct ggccgatcga
     2161 gccgaaccac gtcgtcgtgc acggtttcga cccgcccgcg acccggtgga gcagcggaca
     2221 ccgaggcgtc gacatcgctg cggcggtcgg gaccagcgtc ctcgcgccga ccgacggaca
     2281 ggtgagcttc gtcggcatgg tcgccgggcg tccggtgctc gtcctcacac atgccggcgg
     2341 gcttcggtcg accttcgagc cggtgtcgac gacggccgcc gtgggtcaac tggtgcgccg
     2401 cggcgaggcg gtcgggacgg tcgcggcata cccagggcat tgcgcgccgg ccagttgcct
     2461 gcattggggg gtgttgcgcg gcgccaccta cctcgatccg ctcgccctgc tcgccggacg
     2521 ggtgatcctg ctgccgatgc gatagggcgc gggttactgc tgggtgggcc agggccaggc
     2581 ccggcagcct tcgagcccga gggtctgttg aaccatgatc ggcgccggtc gaccaggggc
     2641 aggacaagag acgtgtcggt gacccagccc atggccgacc tcatggttga cgaggtaggt
     2701 gcggtaggac gtgaggtcgg tcccgtaggt cgccgagccg cggacccaac gatggaggtt
     2761 gagcacggcg cgaccgcccc accaacacga cacattggct gcggtgacgt tcagcggccc
     2821 acacaacttg ccggtcaggg tgggggtggc gagggtgacg cgcacatcca cgccggcgcc
     2881 cgccgcgatc tgcgccggag acacgggcac gaagcgcact ccatcggctg tctgccatcc
     2941 ctgaggatca ctgaggatcc cggcgaccgt cgcggcgaag agcgcctcgt cgatgctcag
     3001 gccgtcttcg acctcgatgc tcaaccggac cgcacgcccc gcgctcgccc agggcgcgtc
     3061 gggaatggcg atcgggtgca tggtcccctt ggcggaggcc tgcaccgggg cgagcgcggc
     3121 acccggggcg gcggtcggtt cgggggcgcc ttcctcgacc tcacccatcg actcggacgg
     3181 ggcgggctcg gacgctgtgc cggtggacgc cgtcgggggg actgctt
//
LOCUS       DAOMZY010000379         3216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_29176_length_3216_cov_5.076558, whole genome shotgun sequence.
ACCESSION   DAOMZY010000379 DAOMZY010000000
VERSION     DAOMZY010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29176_length_3216_cov_5.076558"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..416
                     /locus_tag="PLK64_15675"
     CDS             21..416
                     /locus_tag="PLK64_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HPV81234.1"
                     /translation="MPTGKVKWYDAEKGFGFLSDDDGGDVFLHANALPEGVSTLKGGT
                     RVEFGIVEGRRGAQALSVKVLDPAPSVVASQRAAHRKPSEDMVLIIEDLIKLLDNTSE
                     SLRRGKYPDKNHAGKVAQVLRAVAADLEA"
     gene            416..1162
                     /locus_tag="PLK64_15680"
     CDS             416..1162
                     /locus_tag="PLK64_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3027 domain-containing protein"
                     /protein_id="HPV81235.1"
                     /translation="MSQVKSDSALAGAVDLARDAAIAIAEPGAVGAHLEVVMEAERLA
                     THYFACDHTAYRGWRWSVSVARAPRQKFATVCETSLVPGADAILAPEWLPYAARIAPG
                     DVGAGDVLPYREDDPLLQAGFEATGDDEVDQLAFAELGLGRKRVLSPEGRVAAAERWY
                     AGSHGPRSEVAEKATDRCVSCGYYLPMAGALRRSFGVCANEWSPSDGAVVSLDHGCGA
                     HSEIDVEAVPAVVVPEPILDEFSVDILPRD"
     gene            complement(1220..1459)
                     /locus_tag="PLK64_15685"
     CDS             complement(1220..1459)
                     /locus_tag="PLK64_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2530 domain-containing protein"
                     /protein_id="HPV81236.1"
                     /translation="MTEPSAEREPLRPLAVPMRRIVEWGLAAWAVALVLTLLVPSLHT
                     GERSWWPRCAAAGLVLGVIGYAYLRRGRGNAAGAG"
     gene            1651..3147
                     /locus_tag="PLK64_15690"
     CDS             1651..3147
                     /locus_tag="PLK64_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HPV81237.1"
                     /translation="MAKAAPTRTPVPTSGIDRFFKITERGSSVEREIRGGVVTFFTMA
                     YIVVLNPLILGFVKDANGQFLGGGAAPNLAAIAAGTALVAGLMTIFMGVFANFPLALA
                     TGLGLNAFVAFSIATKMTWADAMGLVVLEGIIILVLVLTGFRTAVFHAVPAQLKIAIS
                     VGIGLFIAMIGLVDAGFIRRTGVGPVPVELGVGGTLSGWPVLVFAIGLLLVIGLWVAK
                     VRGAILLSIIITTVLAIIVEAIAKVGPTVIGQNPDGSNKTNPLGWNLNVPALPDKVIA
                     TPDLSTVGQFNLFGSFGKAGFVAAALLVFTLMLADFFDTMGTMTAIGAEAGLNDTEGT
                     PPNTQKILIVDSVAAAAGGAAGVSSNTSYIESASGVGEGARTGLASVVTGALFLLSMV
                     FAPLVEIIPNEAAVPALVLVGFLMMQQVKGIDWDNLEIAIPAFLTIILMPFTYSITVG
                     IGAGFIAYVFIKMVRGKSSAIHPLMWVVSGLFVLYFAIDPLKRILGAG"
BASE COUNT          480 a         1163 c         1053 g          520 t
ORIGIN      
        1 cacgagctcg aggtgagacg gtgcccacag gcaaggtcaa gtggtacgac gcggaaaagg
       61 gtttcggctt cctctccgac gacgatggcg gcgacgtctt tctgcatgcc aacgcgcttc
      121 ctgaaggtgt gagcaccctc aagggcggca cccgggtcga gttcggcatc gttgagggtc
      181 gccgcggcgc tcaggcgctc tcggtcaagg tgctcgaccc ggctccgtcg gtcgtcgcct
      241 cgcagcgggc tgcgcaccgc aagcccagcg aagacatggt cctcatcatc gaagacctca
      301 tcaagctgct cgacaacacg tcggagtcgt tgcgccgggg gaagtacccc gacaagaacc
      361 acgccggcaa ggtcgcccaa gtcctgcgcg ccgtcgccgc cgacctggag gcgtgatgag
      421 tcaggtcaag tccgactcgg cgctcgccgg ggcggtcgac ctggcccgtg acgcagccat
      481 agccatcgcc gagcccggcg ccgtcggcgc tcacctcgag gtcgtcatgg aggccgagcg
      541 gctggcgacg cactacttcg cgtgcgacca cacggcatac cgaggctggc gctggtctgt
      601 gagcgtggcc cgcgcgccgc gccagaagtt cgcgacggtc tgcgagacgt ccctcgtgcc
      661 cggcgccgac gcgatcctcg cacccgagtg gctcccgtat gccgcgcgga tcgcgcccgg
      721 cgacgttggg gcgggcgacg tcctgcccta ccgcgaggac gacccgctgc tgcaggctgg
      781 cttcgaggcc acgggggacg acgaggtcga ccaactcgcc ttcgccgagc tcggcctcgg
      841 tcgcaagcgg gtcctctcac ccgaggggcg cgtcgccgcc gccgagcgtt ggtatgccgg
      901 ttcgcacggc cctcgctccg aggtcgccga gaaggccacc gaccgctgtg tgagctgtgg
      961 ctactacctg ccgatggccg gagcgttgcg ccgctcgttc ggagtgtgtg ccaacgaatg
     1021 gtcgccttcg gacggcgctg tcgtcagcct cgaccacggc tgcggtgcgc actcggagat
     1081 cgacgtcgaa gccgttcccg ccgtcgtggt cccggagccc atccttgacg agttctcggt
     1141 ggacatcctg ccacgcgact gaggtcagcc ggcttctggc tcgtggctca gccggctccc
     1201 cgctcagtcg aaccctggct cagccagctc cggcggcgtt gccgcggccg cggcgcagat
     1261 aggcatagcc gatcacgccg agcaccaacc cggcggcggc acaccgcggc caccaggacc
     1321 gctcgccggt gtgcaggctc gggacgagca gggtgagcac cagcgcgacc gcccaggccg
     1381 ccaggcccca ctcgacgatc cggcgcatcg gcaccgccaa cggccgcagc ggctcgcgct
     1441 cggcgctggg ctcggtcatg acgggcagcc tatccgcagc cggaggggct caaaccggcg
     1501 aggcgggagc aggcggaaag ccagcaggaa gcaggctgga ggcgagtggg ggaggcgtgc
     1561 ggacggcata cagggacaga tcgggcgctg cgcacggcgg gtggtgagcc gacacacgtc
     1621 ggaaacctgc ccgccgctag cctgtttgac atggcgaaag cagcgcccac gcgcacccct
     1681 gttccgacga gcggcatcga ccgcttcttc aagatcaccg aacgcggctc cagcgtcgaa
     1741 cgcgagatcc gcggcggtgt cgtcacgttc ttcacgatgg cctacatcgt ggtcctcaac
     1801 ccgctcatcc tcggcttcgt caaggacgcc aacgggcagt tcctcggcgg cggtgccgcg
     1861 ccgaacctgg cggcgatcgc ggcgggcacg gctctggtcg cgggcctgat gacgatcttc
     1921 atgggggtct tcgccaactt cccgctggcg ctggcgacgg gcctgggcct caacgccttc
     1981 gtcgccttct ccatcgccac gaagatgacc tgggccgacg cgatggggct ggtcgtcctc
     2041 gaaggcatca tcatccttgt cctcgtgctc accggcttcc ggaccgcggt gttccacgcc
     2101 gtccccgccc aactgaagat cgccatctcc gtcggcatcg gcctgttcat cgccatgatc
     2161 ggcctggtcg atgcgggctt catccgccgc accggcgtcg gcccggtccc cgtcgagctc
     2221 ggtgtcgggg gcacgctgag cggctggccg gtcctcgtct tcgccatcgg gctgctgctc
     2281 gtcatcggcc tgtgggtcgc caaggtccgc ggcgcgatcc tgctgtcgat catcatcacc
     2341 acggtcctgg cgatcatcgt cgaggccatc gccaaggtcg gcccgaccgt catcggccag
     2401 aacccggacg gctccaacaa gaccaacccg ctcggctgga acctcaacgt gcctgcgctg
     2461 cccgacaagg tcatcgcgac gcctgacttg tcgaccgtcg gccagttcaa cctcttcggg
     2521 tcgttcggca aggccggctt cgtggccgcg gcgctgctcg tgttcaccct catgctcgcc
     2581 gacttcttcg acacgatggg cacgatgacc gccatcggcg ccgaggccgg gctcaacgac
     2641 accgagggca cgccgcccaa cacccagaag atcctcatcg tcgactcggt cgccgccgcc
     2701 gcgggtggcg ctgcgggcgt ctcgtccaac acgtcctaca tcgagtcggc gtccggcgtc
     2761 ggtgagggcg cacgcacggg tctggcgtcc gtcgtcaccg gcgcactgtt cctgctgtcg
     2821 atggtcttcg ccccgctcgt ggagatcatc cccaacgagg ccgcagtgcc cgcgctcgtg
     2881 ctcgtcggct tcctcatgat gcagcaggtc aagggcatcg actgggacaa cctggagatc
     2941 gccatcccgg cgttcctcac gatcatcctc atgccgttca cctactcgat caccgtcggt
     3001 atcggggccg gcttcatcgc ctacgtcttc atcaaaatgg tccgcggcaa gtcctccgca
     3061 atccacccgc tgatgtgggt cgtctcgggg ctcttcgtcc tgtacttcgc catcgacccg
     3121 ctcaagcgga tcctcggcgc cggctgatcg accggcccca cccagcacca ccaccgacgg
     3181 ccggtatgcc gcgggcgcga gccacgcggc ataccg
//
LOCUS       DAOMZY010000380         3214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_29203_length_3214_cov_5.178854, whole genome shotgun sequence.
ACCESSION   DAOMZY010000380 DAOMZY010000000
VERSION     DAOMZY010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29203_length_3214_cov_5.178854"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PLK64_15695"
     CDS             complement(<1..738)
                     /locus_tag="PLK64_15695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HPV81238.1"
                     /translation="MPASLSFAAYGEGIGNASAVVRANAVSAGLTAPVPSCPGWTVLD
                     LVLHLGTAHRWASAIIEGVPQDRWPTEKSGRAEGLASGDVLEWFDDGMAHLLHVLASA
                     PADLKAFFFLKDAGRPREAWARRQAHETTIHGVDAMAARLGRVPTASQTWVTAALAAD
                     GVDELLTGFVPRKSGKLRTAPGEPPLTVLVRATDIDRAWTLRVSADPVITTTGTRAQE
                     EAHGIPDVVWEGTAAALYLGLWNRGDEI"
     gene            864..1811
                     /locus_tag="PLK64_15700"
     CDS             864..1811
                     /locus_tag="PLK64_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HPV81239.1"
                     /translation="MEASDLLRTSLLQLAKMHRVRDVVERAPVSRAVVRRFVAGSAVA
                     DAVRVAGELAAVGRSSTIDYLGEDTTQLDQARHTRDTYLTLLRELSARGLSHGGKAEV
                     SVKLTALGLHLPDGPAVALGHAREIAQAAVNAGTTLTVDMEDHTTTDSTLRIVAQLRE
                     DFPSVGAVLQAYLHRTEADCRELANAGSRIRLCKGAYQEPETVAYQDGAEVDRAYVRC
                     LKILMAGDGYPMVASHDPRLVEIAAALAGRAGREPKTFEYQMLYGIRPDEQQRIADRG
                     DQMRVYIPFGEEWYGYLMRRMAERPANVAFFLRGVATKG"
     gene            1938..2744
                     /gene="proC"
                     /locus_tag="PLK64_15705"
     CDS             1938..2744
                     /gene="proC"
                     /locus_tag="PLK64_15705"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HPV81240.1"
                     /translation="MATTAIFGAGVMGETLLSGLLRSGRPADDIVITEKRGDHAATLR
                     ERYGVRVLDNAQAAEVAEVLVLVVKPQDMDGLLGEIREHIAPGNLVVSLAAGIPTAYL
                     ESRLPEGSSVVRVMPNTPALVDQGMAAISPGRNCTADHLAEAEALLASCGKVLQIAEK
                     HQDAVTAISGSGPAYIFYVVESMIEAGVLLGLPRATSTELVVQTLYGAATMLRETGEH
                     PTVLRERVSSPGGTTMAAIRQLEDHKVRAAFMTAMEAAAARSAELAAGLT"
     gene            2741..>3214
                     /locus_tag="PLK64_15710"
     CDS             2741..>3214
                     /locus_tag="PLK64_15710"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV81241.1"
                     /translation="MTDAVSAGLPVGLPAALSVVRLAPDDWEVHRDLRLEALATDPEA
                     FGATYADNAAYDEATWRARLAAVTYWQVREAGMPLGMVGLWDPLADGVADEAGVQGDA
                     GEGADDERVPFVIGMFVRAAARGRGVGSALIDQAIAEAGERGCAQLALDVHAGNTA"
BASE COUNT          472 a         1107 c         1170 g          465 t
ORIGIN      
        1 gatctcgtca cctcggttcc acaacccgag gtagagcgcc gccgcagtgc cttcccacac
       61 gacatccggt atgccgtgcg cctcctcctg cgcgcgggtt ccggtcgtgg tgatgaccgg
      121 gtcggcagac acccgcaacg tccaggcccg gtcgatgtcg gtggctcgga ccaacaccgt
      181 caacggcggc tcgccgggag cggtgcggag cttgccggac ttgcgcggga cgaacccggt
      241 gagcaactcg tcgaccccgt cggcggccaa cgctgccgtc acccaggtct ggctggcggt
      301 gggaacccgg cccaggcggg cagccatggc atccacgccg tggatcgtcg tctcgtgcgc
      361 ctgccgcctg gcccaggcct cgcgtggccg tccggcgtcc ttgaggaaga agaacgcctt
      421 gaggtctgcg ggtgcgctgg ccaggacgtg caggaggtgg gccatgccgt cgtcgaacca
      481 ctccaggacg tcgccgctgg ccaaaccctc cgcgcggccc gacttctcgg tcggccagcg
      541 gtcctgcggc acgccctcga tgatggcgct cgcccaccgg tgggccgtgc cgaggtgcag
      601 gaccaggtcg aggacggtcc acccggggca ggacgggacg ggcgcagtga ggccggcgct
      661 cacggcgttg gctcgcacga cggccgaggc gttgccgatc ccctccccgt aggcggcgaa
      721 ggacaggctc gcaggcatga agcaactgta cgagaccgac ccagcgccgt cacggccgcg
      781 cgtcgccacc gcgcgttgcc agcgtgggtt ccgcgagcgc ttcgacaccc caccaccccc
      841 gcggcggtcg tagagttgtc gccgtggaag ccagtgacct cctgcgcact tctctcctgc
      901 agttggccaa gatgcaccgg gtgcgcgacg tcgtcgagcg ggcgccggtc agccgagcgg
      961 tcgtgcgacg cttcgtcgcc ggatcggcgg tggccgacgc ggtccgggtg gccggcgagc
     1021 tcgccgcggt cggccggtcg agcaccatcg actacctcgg cgaggacacc acccaactgg
     1081 accaggcgcg gcatacccgc gacacctatc tcaccctcct gcgcgagttg tccgcgcggg
     1141 gtctgagcca cggtggcaag gccgaggtgt cggtgaagtt gaccgccttg ggcttgcacc
     1201 tgcccgacgg ccccgcggtc gcgctgggcc acgcgaggga gatcgcccaa gcggcggtca
     1261 atgccgggac gacgctgacc gtcgacatgg aagaccacac gacgaccgac tcgacgctgc
     1321 gcatcgtcgc gcaactgcgt gaggacttcc cctccgtggg ggccgtgctg caggcctacc
     1381 tgcaccgcac cgaggccgac tgccgcgagt tggcgaacgc gggctcgcgg atccggttgt
     1441 gcaagggggc ctaccaggag ccggaaacgg tcgcctacca ggacggggcc gaggtcgacc
     1501 gggcctacgt gcgctgcctg aagatcctca tggccggcga cggatacccc atggtggcca
     1561 gccacgatcc tcggctcgtc gagatcgccg cggcactggc tggccgggcc gggcgggagc
     1621 ccaagacctt cgagtaccag atgctctacg gcatccgccc cgacgagcag cagcgcatcg
     1681 ccgaccgcgg cgaccagatg cgcgtctaca tccccttcgg cgaggagtgg tacggctacc
     1741 tcatgcgccg gatggccgag cgccccgcca acgtcgcgtt cttccttcgc ggggtggcca
     1801 ccaagggctg agggtatgcc gcgcgccgat aaagctcggc aggcagactc tcccggtcgg
     1861 taggctcgcg gcgccgacgt gcggcggcag aatcgttgcg cgggaactcg gcgagacctc
     1921 gacggaagtg acacatcatg gcgacgacgg cgatcttcgg agcgggcgtg atgggggaga
     1981 ccctgctgtc cgggctgctg cgctcggggc gtccggccga tgacatcgtc atcaccgaga
     2041 agcggggcga ccatgccgca accttgcggg agcgctacgg cgtgcgggtc ctggacaacg
     2101 cgcaggcggc cgaggtggcc gaggtcctcg tgctcgtcgt caagccgcag gacatggacg
     2161 gcctgctcgg ggagatccgt gagcacatcg cgccgggcaa cctggtcgtg tccctggcag
     2221 ccggcatccc cacggcatac ctcgagagtc ggctgcccga gggctcctcg gtggttcggg
     2281 tcatgcccaa cacgcctgcc cttgtcgacc aggggatggc ggcgatctcc ccgggacgca
     2341 actgcaccgc ggaccacctc gccgaggcgg aggcactgct cgcctcctgt ggcaaggtcc
     2401 tgcagatcgc ggagaagcac caggacgccg tcacggcgat ctccggcagc ggaccggcat
     2461 acatcttcta tgtcgtcgag tcgatgatcg aggcgggggt gctgctgggt ctgccgcgcg
     2521 ccacctcgac cgagctcgtc gtgcagaccc tttacggggc ggcgacgatg ctgcgtgaaa
     2581 cgggcgagca cccgaccgtc ctgcgcgagc gggtgtccag ccccggcggc acgacgatgg
     2641 cggcgatccg ccaactcgag gaccacaagg tgcgggcagc cttcatgacc gctatggagg
     2701 cggccgcggc gcgctcggcg gagctcgctg ccggactgac atgacggacg cggtgtcggc
     2761 gggtctcccg gtgggtctcc cggcggcgct ctcggtggtt cggctggccc ccgatgactg
     2821 ggaggtccat cgggatctgc gcctggaagc tctggcgacc gaccccgaag ccttcggcgc
     2881 gacctacgcc gacaacgcgg catacgacga ggcgacctgg cgcgcgcggt tggcggcggt
     2941 gacctactgg caggtccgcg aagcgggtat gccgttgggc atggtcggtc tgtgggaccc
     3001 gctcgccgat ggtgtcgccg acgaggctgg ggtgcagggt gatgcggggg agggtgctga
     3061 tgacgagcga gtgccgttcg tcatcgggat gttcgtccgc gccgcggcgc gtgggcgcgg
     3121 tgtgggctcg gccctcatcg accaggcgat cgccgaggcg ggtgagcgcg gctgtgcgca
     3181 actggccctc gacgtccacg ccggcaacac cgcg
//
LOCUS       DAOMZY010000381         3198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_29421_length_3198_cov_5.836144, whole genome shotgun sequence.
ACCESSION   DAOMZY010000381 DAOMZY010000000
VERSION     DAOMZY010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29421_length_3198_cov_5.836144"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="PLK64_15715"
     CDS             <1..1089
                     /locus_tag="PLK64_15715"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HPV81242.1"
                     /translation="GSSDNLVCSPYSVAAALAMTANGARGKTATEMLAVLGGLTVDDL
                     DAGLAALSATFASRAGAKTKSDGNKADIALDIANSLWGQRGTTWEQPFLDALAKWFGA
                     GMNLVDYVAATEAARARINGWTAAATHNKINDLLPEGALDPSTRLVLVNAVYLKAPWE
                     EPFAASATQKAPFTLAAGGTVQADMMRGSFERASAATGPGYTAVSLPYAGGELAMTLV
                     LPGRSNSESLIEWLKSGLLSAALADLRPRPVNLAMPKWTFRSRLALKPVLSALGMPSA
                     FEGSADFSGITREVQLAIDEVFHQGFVAVDEEGTEAAAATAVVMSEVSAPADPLQVTL
                     DRPFLFVVHDVASRTPLFIGRVADPTRT"
     gene            1157..1438
                     /locus_tag="PLK64_15720"
     CDS             1157..1438
                     /locus_tag="PLK64_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HPV81243.1"
                     /translation="MHGYSERKDAHLKRLRRIEGQVRGVSRMVEEDQYCIDVLTQLSA
                     VTRALQSVALGLLDDHMAHCVVAAAREGGVEQETKLAEASAAIARLVRS"
     gene            1453..3057
                     /locus_tag="PLK64_15725"
     CDS             1453..3057
                     /locus_tag="PLK64_15725"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HPV81244.1"
                     /translation="MPRTSPPSVPASPTASASSPREAPFAVIVQAADAVAATTSRTAK
                     IGLLADVLGRVAVDEVEPAIGFLTASVRQGRLGVGWRTIADLAERASAPTDHASLTVT
                     EVDAAIERLAALGGTGSAAARTEELTRLWGRATADEQRLLTGILLGELRIGALEGVLT
                     DAVAKAAGRPLEEVRRAAMLTGSLGRTAYLALTGADLARVGLTVGVPLLPMLAATASS
                     PGEAVTALGPAPVSVEVKLDGARIQVHRHGGPGSRVEVFSRTLADITARVPELVEVVS
                     GFPGGSLILDGETLTLDENGAPRPFQETLSRFGSTGARAEILRPWFFDILHADGVDLI
                     DEPLSVRAERLREAVGEHGILALETADPVAAETFSQEALAAGHEGVLVKALDSPYAAG
                     RRGRQWLKVKPVHTYDLVVLAAEWGSGRRTGWLSNLHLGARDPGVPVGEPGAFVMVGK
                     TFKGLTDEVLRWQTTRLLELETKRTRYAVHVTPALVVEIAIDGVQRSSRYPGGLALRF
                     ARVKGYREDKAVADADTIEALRALLR"
BASE COUNT          454 a         1109 c         1145 g          490 t
ORIGIN      
        1 gggagttccg acaacctcgt ctgctcgccc tattcggtgg cagctgcgct cgcgatgact
       61 gccaacggtg cccgcggcaa gaccgcgacc gagatgcttg ccgtcctggg cgggttgacg
      121 gtcgacgatc tcgatgccgg gctggctgcg ctgtcggcga ccttcgccag ccgcgccgga
      181 gccaagacca agtccgacgg caacaaggcc gacatcgccc tggacatcgc caactccctg
      241 tggggacaac ggggcacgac gtgggagcag ccgttcctgg acgccctggc gaagtggttc
      301 ggcgccggga tgaacctcgt cgactacgtg gccgccaccg aagccgcccg cgctcgcatc
      361 aatggatgga cggcggcggc gacccacaac aagatcaacg atctcctccc ggagggtgcg
      421 ctcgacccgt cgacccgact cgtcctggtc aatgccgtat atctcaaggc gccgtgggag
      481 gagcctttcg ccgcctcggc cacccagaag gcgcccttca ccctggcggc cggcggcacc
      541 gtgcaggccg acatgatgag gggctcgttc gagcgggcgt cggcggccac cggccccggc
      601 tacaccgccg tctcgctccc gtatgccggg ggcgaacttg ccatgaccct cgtgctcccg
      661 ggccgctcaa actccgagtc cctcatcgag tggctcaagt ctggcctcct gagcgcggcg
      721 ctcgcagacc tgcgcccgcg cccggtcaac ctcgccatgc cgaagtggac cttccgcagt
      781 cgccttgcgc tcaagccggt gttgtcggcg ctggggatgc cgagcgcttt cgaggggtca
      841 gccgacttct cagggatcac ccgagaagtc caactcgcca tcgacgaggt gttccaccag
      901 ggattcgtcg ccgtcgatga ggaaggcacc gaggccgctg ccgccactgc tgtggtcatg
      961 agtgaggtct cggcgcccgc ggatcctctc caggtgacct tggaccgccc gttcctcttc
     1021 gtcgtccacg acgtcgccag tcgcacaccc ctgttcatcg gacgcgtcgc cgacccgact
     1081 cgcacctagg tcgcgccaca ccgcgaacgg gagctgcgcg gcataccctg tgggggtatc
     1141 acgagcagga ggcgacatgc acgggtattc cgagcgcaag gatgcgcacc tcaagcggct
     1201 gcgacgtatc gaggggcagg tccggggggt gtcccggatg gtcgaggagg accagtactg
     1261 catcgatgtc ctcacccagt tgtcggcggt gacccgcgcg ctgcagtccg tcgcgctggg
     1321 gctgctcgac gaccacatgg cgcactgcgt cgtcgccgcc gcgcgggagg gcggcgtcga
     1381 gcaggagacc aaactcgcgg aggcctccgc agccatcgcg cgcctcgtgc gcagctgagt
     1441 tccttcgccg gtatgccgcg cacctcgccg ccgtctgtgc ccgcgtcccc gaccgcctcg
     1501 gcgtcgtcgc cgcgtgaggc gccgttcgcc gtgatcgtcc aggccgctga cgccgttgcg
     1561 gcgaccacct cgcggacggc gaagatcggg ctgctggccg acgtcctggg cagagtggcg
     1621 gtcgacgagg tcgagccggc gatcggcttc ctcaccgcct cggtgcgcca ggggcgcctg
     1681 ggtgtcggct ggcggaccat cgccgacctc gccgaaagag cctcggcccc caccgatcac
     1741 gcctctctca cggtcaccga ggtcgacgcc gcgatcgagc ggctggccgc tctcggaggc
     1801 accggatcgg cggcggcgcg gaccgaagaa ctcacccggc tctggggtcg cgcgacggcc
     1861 gacgaacagc ggttgctcac gggaatcctc ttgggagagt tgcggatcgg ggcgctagaa
     1921 ggggtgctca ccgatgcggt ggcgaaggct gccgggcgcc ctctggagga ggtccggcgg
     1981 gcggcgatgc tgaccgggag cctggggcgg acggcatacc tcgcgctgac cggtgccgac
     2041 ctcgcacggg tgggcctcac ggtgggcgtc cccctgctgc cgatgctggc ggcgacggcc
     2101 tcctcgcctg gcgaagcggt gacggcactc gggccggcgc ccgtctcggt cgaggtcaag
     2161 ctcgacggcg ctcggatcca ggtgcaccgc catggcgggc cgggcagtcg ggtcgaggtg
     2221 ttcagccgga ccctggccga catcaccgcg cgggtgccgg agctcgttga ggtcgtgtcc
     2281 ggcttcccgg gcggatcgct catcctcgac ggcgagacgc tcacgctcga tgagaacggc
     2341 gcgccgcggc cgttccaaga gacgctgagc cgcttcggct cgacgggtgc gcgcgccgag
     2401 atcctgcggc cgtggttctt cgacatcctg cacgccgacg gtgtcgacct catcgacgag
     2461 ccgttgtcgg tgcgtgcgga gcggctgcgc gaggcggtgg gggagcacgg catactcgcg
     2521 ctggagacgg ccgaccccgt tgcggcggag acgttttcgc aggaggcgct cgccgccggt
     2581 cacgaaggcg tgctggtcaa ggcgctcgat tccccgtatg ccgcggggcg ccgcggccgt
     2641 cagtggctca aggtcaagcc ggttcacacc tacgacctcg tcgtgcttgc ggccgagtgg
     2701 ggctcggggc ggcgcaccgg gtggctgtcg aacctccacc tcggagcgcg cgacccgggg
     2761 gtgccggtcg gggagccggg cgccttcgtc atggtcggca agacgttcaa ggggctcacc
     2821 gacgaggtgc tccgttggca gacaacgaga ctgctcgagc tggagaccaa gcgcacccgg
     2881 tatgccgtgc acgtcacccc cgcgctcgtc gtcgagatcg ccatcgacgg cgtgcagcgc
     2941 tcgtcgcgct atccgggtgg gctggcgctg cggttcgcgc gggtcaaggg ctatcgggag
     3001 gacaaagcgg tggccgatgc cgacaccatc gaggcgttgc gcgcgctcct gcgctgagcg
     3061 agagggccag agccgcgtgg aggcacgcga cgcatctcag gtgtggctga gcgggctcga
     3121 ctcgccgatg atcaactcgg gcgccaaccg gatgtcctgg atggcagccc gacggcctct
     3181 agcgccgctc tcgctggg
//
LOCUS       DAOMZY010000382         3193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_29486_length_3193_cov_4.530911, whole genome shotgun sequence.
ACCESSION   DAOMZY010000382 DAOMZY010000000
VERSION     DAOMZY010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29486_length_3193_cov_4.530911"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PLK64_15730"
     CDS             <1..539
                     /locus_tag="PLK64_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81245.1"
                     /translation="ESIVGNLTAQLSSRVRWTETMAYLESLRISALVEIGPKQVLANL
                     MRSEKPLIRSFAMDIAADREAWADYRVPTLDEVSLIGRCLAVAVCTRNHNFDDEQYRR
                     GVIEPYNEIKALQDFLELEQRLPDRQDSMRALLLLKTILDTKGTPPEEQRSRVVQITQ
                     ETGTYGSLRTDLQTAGLL"
     gene            572..>3193
                     /locus_tag="PLK64_15735"
     CDS             572..>3193
                     /locus_tag="PLK64_15735"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPV81246.1"
                     /translation="MDFSAGPVFGARSDSESSGSGGFGTPEPIAIIGAAGRFALAADL
                     EEYWANLRYGRNGHRRFPGDRARAGARAARDKAGAGLGTSYPAGYLDDIAAFDHAFFE
                     ISFREACLMDPNQRIFLEEAYRALESAGYTGPALRDFDTGVYVGHSADLTAAYADFVR
                     DEHPTVFPEISVAGNMSSVIAGRLAYHLDLRGPCVVVDTACSSSLVAVHIASEALRSG
                     QIRMAVVGSVKVNIAPLGPGPDDELGIRSAHHQTRTFDAAADGISSGEGAAAVVLKRL
                     DDALADGDRILAVIRASAVNQDGRTVGITAPNPRAQEELLSGCWEAAGIDVEDLVYLE
                     AHGTGTTLGDPIEVSAMSAALRRHTSRRGFCAVGSVKPSLGHLDHLAGLASLVKVVAM
                     LGHRTIPPTIHFETPNRHIDFVDSGLYVADVLHPMPAEVERPLCGVSSFGLAGTNCHV
                     LLEHYPPTVTTGSPGPHLLAISAKTSAQRSALVGDYIVALTASQGIDDDLAAVCRTAA
                     DGRPAFPMRLACVADSSAQLVDALQRWLADDAEPSVLANSVRAREVPSGGDLPESVPR
                     DGAARRAVLTDVARRFVAGDDSALALVREPGEGRSRLPHHPLRRIPCWAAEIDQPPEG
                     VRLERLVDTGDETFFRIALDLSTDWVLAEHRVDSDCVLPGAAIVDAVVTAVRTRGRGS
                     AGPIVLERVFFLRPVTVPLGSSAALMVALTRDHSGEAFRVLGSIDGGPWDTHVIGAVR
                     YPEDAVAADIAVPPDTALRLVGPDEERPVAVSIGGHWTRISRELREQDAMTSWGRFHV
                     PDSDAAAFAGFHLYPPLLDRALNALNTHIGSGTYLPFSVDRLVVHRPLDRTCVARLTR
                     RPSDGLDRSLLLDIVLTD"
BASE COUNT          469 a         1128 c         1067 g          529 t
ORIGIN      
        1 aggagagcat cgtcggcaac ctcaccgccc agctctccag ccgggtccgg tggaccgaga
       61 cgatggcgta cctcgagtcg ctgcggatct cggcgctggt cgagatcggc cccaagcagg
      121 tgctcgccaa cctcatgcgc agcgagaagc cgctgatccg cagcttcgcc atggacatcg
      181 ctgcggaccg agaggcctgg gcggactacc gggtcccgac gctggacgag gtctcgctca
      241 tcggtcgctg cctggccgtc gcggtctgca cccgcaatca caacttcgac gacgagcagt
      301 accgacgcgg cgtgatcgag ccctacaacg agatcaaagc gctgcaggac ttcctggaac
      361 tggagcagcg tctccccgac cggcaggact cgatgcgcgc cctgttgctg ctcaagacga
      421 tcctcgacac caaggggaca ccccctgagg agcagcgctc gcgagtcgtg cagatcactc
      481 aggagaccgg cacctacggg tcgttgcgga ccgacctgca gaccgctggt ctcctctagg
      541 agggtgggac gggcgaatga ctgtcggggc gatggacttc tcagccggac cggtgttcgg
      601 cgccaggtcg gactctgagt cgtcgggaag cggtggcttc gggacgccgg agccgatcgc
      661 catcattggt gcggccggcc ggttcgcgct ggctgcagac ctcgaggagt actgggccaa
      721 cctccgctac ggacgaaacg gccatcgtcg gttcccgggc gaccgggcgc gcgcgggggc
      781 gagggcggcg cgcgacaagg cgggcgccgg actcgggacg agctacccgg ccgggtatct
      841 cgacgacatc gccgcgttcg accatgcctt cttcgagatc tccttccgcg aggcctgcct
      901 catggatccc aaccagcgga tctttctcga ggaggcgtat cgagcgctgg agtcagcggg
      961 ctacaccggc ccggccctac gcgacttcga caccggcgtc tatgtcggtc acagcgcgga
     1021 tctcaccgct gcttatgcgg acttcgttcg cgacgaacac cccaccgtgt ttccggagat
     1081 ttccgtcgcc gggaacatga gttcggtcat cgccggccgg ctggcctatc acctcgacct
     1141 ccggggccct tgcgtcgtcg tcgacacggc ctgctcgtca agcctggtcg ccgtccacat
     1201 cgcctcagaa gcgctgcgca gtgggcagat ccggatggcc gtcgtcggga gcgtcaaggt
     1261 caacatcgcc cccctcgggc ccggccccga cgacgagttg gggatccgct cagcccacca
     1321 ccagacccgg acgttcgacg ccgcggccga tggaatcagc tcgggtgagg gtgcggcggc
     1381 cgtggtcctc aagcgactgg acgacgctct cgccgacggg gatcgcatcc tggcggtgat
     1441 ccgcgcgagc gccgtcaacc aggacgggcg cacggtcgga atcaccgctc ccaacccccg
     1501 tgcccaggag gaactgctct cgggttgctg ggaggcagcc gggatcgatg tggaggacct
     1561 ggtctacctg gaagcccacg gcacgggaac cacactgggg gacccgatcg aggtgagcgc
     1621 gatgagtgcc gcgctgcgac ggcatacctc tcgtcggggt ttctgcgcgg tcggctcggt
     1681 gaagccgtcc ctgggacacc tggaccacct tgccggcctc gcgtccctcg tcaaggtcgt
     1741 cgcgatgctt ggtcaccgga cgatcccccc gacgatccat ttcgagactc ccaaccgaca
     1801 catcgacttc gtcgactcgg ggctctatgt ggccgacgtc ctgcacccca tgccggcgga
     1861 ggtcgagcgc ccgctgtgtg gggtgagctc cttcggcctc gccggaacga actgtcacgt
     1921 cctcctcgag cactaccccc cgaccgtcac caccggttcc cccggacctc acctgctggc
     1981 gatctcggcc aagacctcgg cgcagcgttc ggcactggtg ggggactaca tcgtcgcgct
     2041 caccgcgtca caggggatcg acgacgacct cgccgcggta tgccgcacgg cggccgatgg
     2101 gcgccccgcc ttcccgatgc gactggcctg cgtcgccgac tcgtcggcgc agttggtcga
     2161 cgcgctccag cggtggctgg ccgacgacgc cgaaccgtcc gttctggcca attccgttcg
     2221 cgcccgcgag gttccgtccg gtggcgacct gcccgaatcg gttccgcgcg acggtgctgc
     2281 ccgccgtgcc gtgctgacgg acgtggcgag gcggttcgtt gccggcgacg acagtgcgct
     2341 ggcgctcgtg cgcgaaccag gggagggacg atcccgcctt ccgcatcatc cgctccggcg
     2401 catcccctgc tgggcggcgg agatcgacca gccgccggaa ggtgtgcgcc tggaacgtct
     2461 tgtcgatacc ggcgacgaga ccttcttccg gatcgccctg gacctgtcca ccgactgggt
     2521 gttggccgag caccgggtcg actccgattg cgtgctaccc ggcgccgcga tcgtcgacgc
     2581 cgtggtgacc gcggtgcgca cccgcggtcg gggatcggcc ggcccgatcg tccttgagcg
     2641 ggtcttcttc ctgcggcccg tgacggtccc gttgggcagc tcggcggcgc tgatggtggc
     2701 gttgacccgg gatcactcgg gggaagcctt ccgggtcctg gggagtatcg acggcggccc
     2761 atgggacacc catgtcatcg gggcggtgcg atacccggag gacgccgtcg cggccgacat
     2821 cgccgtgccg ccggataccg cattgcggct ggtcggcccc gatgaggagc gacccgtggc
     2881 cgtctccatc ggcgggcact ggacccggat cagtcgggag ttgcgcgagc aggacgcgat
     2941 gacgtcctgg ggtcgattcc acgtgcccga ctcggacgca gccgctttcg cggggttcca
     3001 cctctaccca ccgttgctcg atcgggctct caacgcgctc aacacccata tcggctccgg
     3061 cacctacctc ccgttctcgg tggatcgcct cgtcgtccat cgccccctcg accgcacctg
     3121 cgtggcccga ctgacccgcc gtcccagcga tgggctggat cgctccctgc tgcttgacat
     3181 cgttctgacc gac
//
LOCUS       DAOMZY010000383         3186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_29576_length_3186_cov_6.687640, whole genome shotgun sequence.
ACCESSION   DAOMZY010000383 DAOMZY010000000
VERSION     DAOMZY010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29576_length_3186_cov_6.687640"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            320..502
                     /locus_tag="PLK64_15740"
     CDS             320..502
                     /locus_tag="PLK64_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81247.1"
                     /translation="MTAARARDEMVGLDGTTRLSGAALTVVRDEMAKGPRAPVVMLLV
                     PALVARTGRLLGVGLG"
     gene            547..1311
                     /locus_tag="PLK64_15745"
     CDS             547..1311
                     /locus_tag="PLK64_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81248.1"
                     /translation="MSSRQLDRSVRAQLRTLSQENAEGVGAHLVMVAQLLDSNIQAAE
                     AHAETAVRRAGRVPAAREALGLVAYRKGDWARALNEFRTVRRLSGSSHLLPLMVDCER
                     ALGRPDRALDLAASPEVGSLEQAERIELAIVVSGIRRDAGQFESAAAGLETPTLTSAA
                     RRPWTARLRYAYAEAKLALGDVDAARLWFARAADADDDLATDAAERLEELDGIAYTDL
                     LDGDEGGDPDADVSDDVTDDEGQGPTDSAGRFGGRR"
     gene            1325..2410
                     /locus_tag="PLK64_15750"
     CDS             1325..2410
                     /locus_tag="PLK64_15750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HPV81249.1"
                     /translation="MGGASGQPSLTAAYLGVICDLDGVVYRGPDAIPYAVSALNDVFR
                     LRPLCFATNNASRTPGEVATQLRSLGLTVEDAAVVTSAQAGAQLLAAELPSGSAVLAV
                     GGPGVPAALAAVGLVVTREPEEARAVLQGWGRDVRVDDLARAAVAIAHGARWVATNTD
                     RTLPTPQGLVPGNGTLVSAVAEATGREPEVVGKPHAPLYLAAAERLAAAPERLLAVGD
                     RLDTDIIGATAVGADSLWVLGGVDGFLSLVGSPARPTYAAHDLRALLDPPVRVERLDP
                     ASWACGDVRLSIVSGRVSAAADGVTIDMSAVPGNQLAALAATVVLDLRGQSASYDLAV
                     SLATEFDRVLGHSAGVNRHRVEDTRPQ"
     gene            2442..>3186
                     /locus_tag="PLK64_15755"
     CDS             2442..>3186
                     /locus_tag="PLK64_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81250.1"
                     /translation="MSFDGIRGYVQFATGIGDATRARAAEVAQGLVTVLSRTSPSEMA
                     GQVSTLAEELLSAAKSNRESLIHLIRGEVSELIESSSSLAKVADLDSMRAALTQLGSD
                     LDGLRQQVGVQSPAALVSSGAAAVVTAASSVRPGGSRPAPKPAARSDVPAKVKRVPSR
                     PRGSAASTANDAPVVAPQASAVEGPVVRKVSPVSAPEAPSAPEVRKLTAKKAAPAKKV
                     APEKVAAKKAAPAKKAVVKKAAPAKVAQAP"
BASE COUNT          457 a         1022 c         1172 g          535 t
ORIGIN      
        1 ttcagcgccg atggtactgc actggtgacg gtgtgggaga gtaggacgca gccgaacata
       61 ctttaacgag ggcgcccccg gagaaatccg gctgggcgcc ctcgtgtcgt ttgctgggct
      121 ctacgataga ggccggttca agcccttcgg taggacggat tcgtagtgga caggcccgac
      181 agtggccgcg gtgctcgcgg tggcggctct tcctcatctg gtggtggagc cccgaggcgt
      241 gacgggggcg cgcgtgacgg tggcccccgc cgcgatagcg gagcccgtga cggagccccg
      301 cgtgacggag cgccgaagcg tgacagcggc cagggcgcgc gacgagatgg tgggcctcga
      361 cgggacgact cgtctcagcg gggcggcact gaccgtggtc cgcgacgaga tggccaaggg
      421 cccgcgcgct ccggtggtca tgctcctcgt gccggcgctg gtcgcccgga cggggaggct
      481 cctcggggtg gggctcggct agcgcctaag tcggttcgcc ttccggagcc ggctattccc
      541 gagggtgtga gcagtcgtca actggatcgt tcggtccgcg cccagctgcg cacgctcagc
      601 caggagaacg ccgaaggcgt gggcgctcac ctggtgatgg tggcgcaatt gctcgatagc
      661 aacattcaag ctgcggaggc gcatgccgag accgcggttc gtcgtgccgg tcgggttccc
      721 gccgcacgag aggctttggg tctggtcgcc tatcgcaagg gcgattgggc gcgtgccctc
      781 aatgaattcc gcaccgtccg aaggttgtcg ggctcatccc atctgttgcc gttgatggtc
      841 gattgcgagc gagcgctggg ccggcccgat cgggcgctgg atctcgctgc atccccagag
      901 gtcggatcgt tggagcaggc cgagcgcatc gagttggcga tcgtcgtgtc cggcatccgt
      961 cgggacgccg gccagttcga atcggcggca gcgggtctgg agacccccac gttgaccagc
     1021 gccgcgcgac gaccgtggac cgcgcggctg cgttatgcgt atgccgaggc caaactcgcc
     1081 ttgggcgacg tcgacgctgc gcgactgtgg ttcgcccgcg cagcggatgc tgacgacgat
     1141 ctggccaccg acgctgccga gcggctcgag gagcttgacg ggattgccta cacggatctc
     1201 ttagacggcg acgagggcgg cgaccccgac gcggacgtgt ccgacgatgt cacggacgat
     1261 gagggccaag gccccacgga ttcggccggc cggttcggtg gccggcgtta gtcacgatgg
     1321 ggccgtggga ggggcgagcg gccaaccgtc gctgacggcg gcatacctcg gcgtcatctg
     1381 cgacctcgat ggggtggttt atcggggacc ggacgcgatc ccgtatgccg tctcggcact
     1441 gaacgacgtg tttcgcctgc gtccactgtg ttttgccacc aacaatgcgt cccggactcc
     1501 gggggaggtg gcgacccagt tgcggagcct cggcctgacc gtcgaggacg ctgccgtggt
     1561 cactagtgcc caggccggcg cgcagcttct cgcggctgaa cttccctctg gtagcgcggt
     1621 cctcgccgtc ggtgggccag gggtgccagc agccttggcc gctgtcggac tggtggtgac
     1681 ccgggagcct gaagaggccc gcgcggtgct gcagggctgg ggacgtgacg ttcgtgtcga
     1741 tgaccttgcc agggccgccg tggcgatcgc ccatggcgct cgctgggtgg cgaccaacac
     1801 cgatcgcacg cttcccaccc ctcaagggct ggttcccggc aacggcacgc tggtgtccgc
     1861 ggtcgctgag gccacgggtc gcgaaccgga agtggtcggc aagccgcatg cgcccctcta
     1921 cctcgccgcg gccgagcgct tggccgctgc ccctgaacgg ctcctcgcgg tcggcgacag
     1981 gctcgacaca gacatcatcg gcgcgaccgc ggtcggcgcc gattcgctgt gggtcctcgg
     2041 gggcgtcgac gggttcctct cactggtcgg ttcaccagca cgacccacgt atgccgcgca
     2101 cgacctgcgc gcgctccttg acccgccggt ccgggtcgag cgactcgacc cggcgtcgtg
     2161 ggcgtgcggc gacgtccgcc tgtcgattgt ctccgggagg gtgtcggcgg cggcggatgg
     2221 cgtcaccatc gatatgagtg ctgtgcctgg taaccagttg gccgcgctcg cagcgactgt
     2281 ggtgctcgat ctgcgcggcc aaagcgcgtc atacgacctg gccgtgagtc tcgccactga
     2341 gtttgatcgt gttctcggcc atagcgccgg ggttaaccgc catcgggtcg aggacactcg
     2401 accccagtag cgtgtgggta cacccatcga ggaggagcga catgtcgttt gacggcatcc
     2461 gtggttatgt ccagttcgcg accggcatcg gtgacgcgac cagagcgcgt gccgctgagg
     2521 tggcgcaagg tctggtgacg gtcctgagca ggacgtcgcc gtcggagatg gcgggtcagg
     2581 tgtcgaccct ggccgaagaa ctcctcagtg cggcaaagtc caatcgggag agcctcattc
     2641 acctcattcg tggtgaggtg agcgagctca tcgagtcgtc ctcgtcgctg gccaaggtcg
     2701 ccgacctcga ctccatgcgg gccgccctga ctcagctcgg aagcgacctc gacgggctgc
     2761 ggcagcaggt gggagtgcag tctccggccg cgctggtgtc ctccggtgcc gccgcggtcg
     2821 tcaccgccgc atcgagcgtt cgaccgggtg gttcgcgtcc ggctccgaag cccgccgcgc
     2881 gcagcgacgt tccggccaag gtgaagcgag ttccgtcccg gccgcgcggg tcggctgcca
     2941 gcactgcgaa cgacgcaccg gtcgtcgccc cgcaggcgtc agccgtcgag gggccggttg
     3001 tccgcaaggt ttcgccggtg tcggcccccg aggctccgag cgccccggag gttcgtaagt
     3061 tgacggcgaa gaaggcggcg cccgcgaaga aggtggcacc tgagaaggtg gccgcgaaga
     3121 aggctgcgcc ggcgaagaag gctgtggtca agaaggcggc tcctgcgaag gtggcgcagg
     3181 cgcccg
//
LOCUS       DAOMZY010000384         3186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_29577_length_3186_cov_5.692431, whole genome shotgun sequence.
ACCESSION   DAOMZY010000384 DAOMZY010000000
VERSION     DAOMZY010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29577_length_3186_cov_5.692431"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..286
                     /locus_tag="PLK64_15760"
                     /pseudo
     CDS             1..286
                     /locus_tag="PLK64_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493073.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            297..1331
                     /gene="speB"
                     /locus_tag="PLK64_15765"
     CDS             297..1331
                     /gene="speB"
                     /locus_tag="PLK64_15765"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HPV81251.1"
                     /translation="MSRYGAQFGPDVTFLGVPPCDLEDESTYAGADIVILGAPFDGGT
                     SHRPGTRFGPMAIRTTDYLAHDGSRPSLALRVDGLQDLRVVDAGDVELPPGDIETAMA
                     RIEAAVERIARSGAIPLILGGDHSIAFPDAKGVANVLGHGRISMIHFDAHADTGDIEF
                     GSLWGHGQPMRRLIESGALRGDRFLQVGLRGYWPGPETLDWMAAQRMRSYEMTEIGHR
                     GLATCLTEAFTIATDECDGVFLSVDIDVCDPGHAPGTGTPEPGGLSARELLDSVRRIC
                     LELPVVGIDVVEVSPPYDHADITAALANRVVLEAISAIARKRRDAAYGTSWNPATPLL
                     ADRHQPRQEP"
     gene            1335..2675
                     /gene="gabT"
                     /locus_tag="PLK64_15770"
     CDS             1335..2675
                     /gene="gabT"
                     /locus_tag="PLK64_15770"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HPV81252.1"
                     /translation="MTTPIEQRRILRTAIPGPASAALHERKLASVSAGVSTGLPAYVV
                     AGQGGILVDADGNQLIDFGSGIAVTSVGNGNQRVIDAVSKQIAEFTHTCFMVNPYEQY
                     VAVAEKLNELTPGSHEKRTALFNSGAEAVENAVKVARHFTGKDGIVAFDHAYHGRTNL
                     TLAMTAKNMPYKHRFGPFASELYRVPMAYPYRWPGGPQHCADEAFNYIVDQIHAQIGE
                     ENTAAVIIEPIQGEGGFIVPPAGFLKELAQWCAANNILFIADEVQSGFCRSGDWFASD
                     YEGIVPDLITTAKGIAGGMPLSGLTGRADIMDTIHPGGLGGTYGGNPVACAAALASIE
                     TMEELNLAARAREIEAILRADLEKLAATYPQVGEIRGRGAMIAMELVTDRETKEPAAA
                     LTAAINKACHAQGLVTLTCGTLGNVFRFLPPLVISDDLLHEGMSILADAFAASV"
     gene            2714..>3186
                     /locus_tag="PLK64_15775"
     CDS             2714..>3186
                     /locus_tag="PLK64_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018777331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPV81253.1"
                     /translation="MAMTQGALLESVPKGLFIDGAWRDASDGATLPVDDPATGKILAH
                     VASATVADGKAALDAAVAAQRDWARTAPRDRGEILRRAYEAIIARTDDFALLMTLEMG
                     KPLAEARGEVVYGAEFFRWFSEEAVRIHGRYAVAPNGATRLMTMKQPIGPTLMITP"
BASE COUNT          527 a         1150 c         1029 g          480 t
ORIGIN      
        1 cacctgaagg gacggtgcgc ctcgggccac ctacctctac cacgtggtcg acaacgagtg
       61 gtcgatgcgc gaatacggag cccagtgcgt cgtctggcag accgcaatca accccatcat
      121 cgcgctggaa ctgctcgcca ccggagtgtg gttgggcgcg ggtgtgcgtg gtccggaggc
      181 gttcgacgcg gtgccgttcc tcgacctgtt gtgcactccg aagccagcgg gatacggctc
      241 gccgtgggcg atccaagacc gaacgccgcc tgactcactc tcctaggatt cccaccatga
      301 gccgctacgg cgcgcaattc gggcccgacg tcaccttcct cggcgtcccc ccgtgcgacc
      361 tcgaggacga gagcacgtat gccggtgccg acatcgtcat cctcggcgcg cctttcgacg
      421 ggggcacctc gcaccggccg ggaacccggt tcggccccat ggccattcgc accaccgact
      481 acctcgccca cgacgggtct cggccctcgc tcgcgctgcg ggtcgacggg ttgcaggacc
      541 tgcgtgtcgt cgacgctggc gatgtcgaac tgccgcccgg agacatcgag acagcgatgg
      601 cgcggatcga ggcggccgtc gaacggatcg cccgctccgg ggccatccca ctcatcctcg
      661 gtggcgacca ctcgatcgcc tttccggatg ccaagggtgt cgcgaacgtc ttgggccacg
      721 gccggatctc gatgatccac ttcgatgccc acgccgacac cggtgacatc gagttcgggt
      781 cgttgtgggg acacggccaa ccgatgcggc gactcatcga gtcgggggca ttgcgcggcg
      841 accgcttcct gcaggtcggc ctgcgcggct actggccggg accagagacc ctcgactgga
      901 tggccgcgca gcggatgcgc tcctacgaga tgaccgagat cggccatcgg gggctcgcga
      961 cctgcctcac cgaggccttc accatcgcca ccgatgagtg cgacggggtg ttcctctccg
     1021 tcgacatcga cgtgtgcgac ccgggccacg cgcccggcac aggaaccccg gagcctggcg
     1081 gcctcagcgc ccgcgaactg ctcgactcgg tccggaggat ctgcctggaa cttccggttg
     1141 tgggcatcga cgtcgtcgag gtcagcccgc cctacgacca cgccgacatc actgcggcgc
     1201 tggccaatcg cgttgtcctc gaggcgatct ccgcgatcgc gcgcaaacgc cgcgacgcgg
     1261 catacgggac ctcgtggaac ccggccaccc ccttgcttgc cgatcgacac cagccccgac
     1321 aggagcccta gaagatgacc acccccatcg agcaacgccg aatcctgcgc acggcgatcc
     1381 ccggaccggc ctccgccgcg ttgcacgagc gcaagctcgc ctccgtgtcg gccggggtga
     1441 gcaccgggct gccggcctat gtcgtcgcgg gtcagggcgg catcctcgtg gacgccgacg
     1501 gcaaccagct catcgacttc ggctcgggca tcgcggtgac ctcggtcggc aacggcaacc
     1561 agcgggtcat cgacgcggtg agcaagcaga tcgccgagtt cacccacacg tgcttcatgg
     1621 tcaaccctta cgagcaatac gtcgcggtcg ccgagaagct caacgagctg actcccggca
     1681 gccacgagaa gcgcacggcg ctgttcaact ccggtgccga ggccgtcgag aacgccgtca
     1741 aggtggcgcg gcacttcacc ggcaaggacg gcatcgtcgc gttcgaccac gcctaccacg
     1801 gccggaccaa cctcaccctg gccatgaccg ccaagaacat gccctacaag caccggttcg
     1861 ggcccttcgc gagcgagctc tatcgggtgc cgatggccta cccctaccgc tggccgggtg
     1921 gcccgcagca ctgtgccgac gaggccttca actacatcgt cgaccagatc cacgcccaga
     1981 tcggcgagga gaacaccgcg gccgtcatca tcgagccgat ccagggcgag ggcggcttca
     2041 tcgtgccgcc ggccggcttc ctcaaggagc tcgcgcagtg gtgtgcggcc aacaacatcc
     2101 tgttcatcgc cgacgaggtc cagtcgggtt tctgccgcag tggtgactgg ttcgccagcg
     2161 actacgaagg gatcgtcccc gacctcatca ccaccgccaa gggcatcgcc ggaggtatgc
     2221 cgttgtcggg gctcaccggg cgcgcggaca tcatggacac cattcacccc ggcggcctcg
     2281 gcggcaccta cggcggcaac ccggtggcgt gcgcagcggc cctcgcctca atcgagacga
     2341 tggaggagct caacctggcg gctcgggccc gcgagatcga ggcgatcctt cgggccgacc
     2401 tggagaagct cgcggcgacc tacccgcagg tcggggagat ccgcggccgt ggcgcgatga
     2461 tcgcgatgga gttggtcacc gaccgcgaga ccaaggagcc cgccgcggcc ctcacggccg
     2521 ccatcaacaa ggcgtgtcac gcccagggcc tggtgaccct cacctgcggc accctcggca
     2581 acgtcttccg gttcctgccg cctctggtga tcagcgacga cctgctccac gagggcatga
     2641 gcatcctcgc cgacgcgttc gccgcgtccg tctgaccact gcctgaacta ctgcgacaca
     2701 aggagatcca gccatggcca tgactcaggg tgcacttctg gaatcggtgc ccaagggcct
     2761 gttcatcgac ggggcgtggc gggatgcgtc cgacggtgcg acgctgccgg tcgatgaccc
     2821 agccaccggg aagatcctcg cgcacgtcgc gagcgcgacc gtggccgatg gcaaggcggc
     2881 cctggacgcg gcggttgctg cccagcgaga ctgggcgcgc accgcacccc gcgaccgcgg
     2941 tgagattctc cggcgggcct acgaggcgat catcgcgcgc accgacgact tcgcgctgct
     3001 catgaccctc gagatgggca agccgctcgc cgaggcccgc ggcgaggtcg tctacggcgc
     3061 cgagttcttc cgctggttca gcgaggaggc ggtccggatc cacggccggt atgccgtggc
     3121 ccccaatggc gcgacgcgcc tcatgacgat gaagcagccc atcggcccga ccctcatgat
     3181 cacgcc
//
LOCUS       DAOMZY010000385         3149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_30095_length_3149_cov_8.456044, whole genome shotgun sequence.
ACCESSION   DAOMZY010000385 DAOMZY010000000
VERSION     DAOMZY010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30095_length_3149_cov_8.456044"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..1434
                     /locus_tag="PLK64_15780"
     CDS             268..1434
                     /locus_tag="PLK64_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPV81254.1"
                     /translation="MKIAVVGIGYVGLGNAAVLAARHDVVMLDIDEHKVTQVNDRVSP
                     LDEAELAQFLVTQPLSLKATSDASTAFEGADVVIIATPTDYDPERNFFDTSTVESVAH
                     DVARFAPRATVVIKSTIPVGFTRRLREELPSLSIYFSPEFLREGTALQDCLHPSRIVV
                     GGSDERARQIGALLLEASADPETPVLFTGSTEAEAIKLFANTYLAMRVAFFNELDSYA
                     MSHDLGPREIIEGVGLDPRIGQHYNNPSFGYGGYCLPKDTKQLLANYESVPQNIMGAI
                     VDSNTTRMDFIADDILSREPKVVGLHRLVMKAGSDNFRTSSIQEIMRRLKAEGVTVIV
                     YEPTLDADEFHDCEVVRDVAELKASADIIIANRRTPELDDVPGKVYTRDIYGRD"
     gene            1534..2523
                     /locus_tag="PLK64_15785"
     CDS             1534..2523
                     /locus_tag="PLK64_15785"
                     /inference="COORDINATES: protein motif:HMM:NF023218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPV81255.1"
                     /translation="MKHTFHERQWRHGQRDSVRRRSLLAGGAGVLASAFAACGSPTPT
                     ASPAPVASPTPSTPSLATPSATATPSPTRPPPPPGPLGGVGTWRGAPDASQALADSGA
                     RWFYTWAPEHPEIKAPEGCEFVPMIQDERQLTDSSLALAKANGDTLLGFNEPELALQA
                     NMSVDAALEAWPRLEETGLRLGAPALAGNADNPNSWFAGFMRGVESRNHRVDFVPVHW
                     YLAPGLRESYSLDRAVDDLAGHITRVHDTYGLPIWVSEFSLITWYPARSDAMPPEDQA
                     EFLAAAAEMMTSLAYVERWAWFSLTPPPYAPAVGAYDDSGQITPVGRQIRQLT"
BASE COUNT          540 a         1080 c         1010 g          519 t
ORIGIN      
        1 cactttggtg caatctaggg gcgtgtcagc ccctcaatcg tgaagggccg aggctgacgg
       61 catgctcgac taaccgaaga acccgctttg aactgcggtg atatcgatct tcgatgcgga
      121 tgcgggacgc gactcggtga gccagtgcgc cagagatgga tgttcacggc ccgccgaagt
      181 tcctcgtctg cgggaggttg tccgaacggg tgatgcccac tttgcgcgga cttggtctcg
      241 tctttacctc cgtgttatgt tcccccgatg aagatcgcgg ttgtgggtat cggatatgtc
      301 ggactaggga acgcggccgt gcttgcggcg cgccatgacg tggtcatgct ggacatcgac
      361 gagcacaagg tgacgcaggt gaacgaccgc gtctcgccac tcgatgaggc tgagttggca
      421 cagttcctgg tcacccagcc gctgtcgctc aaggcgacga gcgacgcctc gacggcgttc
      481 gagggagcgg acgtcgtcat catcgctacg cccacggact atgatccgga gcgcaacttc
      541 ttcgacacct ccacggtgga gagcgtcgcg cacgacgtgg cccgcttcgc cccgcgagcg
      601 acggtcgtca tcaagtccac gatcccggtc ggcttcaccc gccggctgcg cgaggagctg
      661 ccgagcctct ccatctactt ctccccggag ttcctgcgcg agggcactgc cctgcaggac
      721 tgcctccacc cgagccggat cgtcgtcggc gggagcgacg agcgtgcacg acagatcggt
      781 gccctgctcc tggaggcctc ggccgacccg gagaccccgg tgctgttcac cggctccacg
      841 gaggctgagg ccatcaagct cttcgccaac acctatttgg caatgcgggt cgcgttcttc
      901 aacgagctcg actcgtatgc gatgtcccac gacctgggcc cgcgggagat catcgagggc
      961 gtggggctgg acccgcggat cgggcagcac tacaacaacc cgagcttcgg ctacggcggg
     1021 tattgcctgc ctaaggacac gaagcagctg ttggccaact acgagtccgt gccgcagaac
     1081 atcatgggcg ccatcgtcga ctcgaacacg acccggatgg acttcatcgc cgacgacatc
     1141 ctctcgcgcg agccaaaggt cgtggggctg caccggctgg tgatgaaggc agggtcggac
     1201 aacttccgca cctcctcgat tcaggagatc atgaggcggc tcaaggccga aggggtcacc
     1261 gtcatcgtct atgagccgac cctggacgcg gacgagtttc atgactgcga ggtcgtgcgt
     1321 gacgtggctg agctcaaggc gagcgccgac atcatcatcg ccaaccggcg cacacccgag
     1381 ctcgacgacg ttccaggcaa ggtgtacacc cgcgacatct atggacgcga ctgaggtcgc
     1441 tcaccagtcg tttgccagga tagatctcgc aagagcgtcc tcagccgccc ctgcagagca
     1501 tccatccgcg tcgagacagc cgggatcccg agaatgaagc acacattcca tgagcgccag
     1561 tggcggcacg gacagcgcga ctccgtgcgt cgccgctccc tgctcgccgg cggggccggc
     1621 gtgctcgcca gtgccttcgc agcctgcggc agcccgaccc ccacggcaag tccggcgcct
     1681 gtcgctagcc ccacgccgtc aaccccgtct ctcgcaaccc cgtcggccac cgccacgccg
     1741 tccccgaccc gtcctccacc ccctcccggc cccctaggtg gggtcgggac gtggcgaggc
     1801 gcaccggacg ccagccaggc cctggccgac tccggcgccc gctggttcta cacctgggca
     1861 ccggagcacc cggagatcaa ggcgcccgag gggtgcgagt tcgtcccgat gatccaggac
     1921 gagaggcagc tcaccgacag ttccctcgct ctggcgaagg cgaacggtga caccctcctc
     1981 ggatttaacg agcccgagct ggccctccag gccaacatgt ccgtcgacgc tgcgctggag
     2041 gcatggcccc ggctcgagga gaccggcctg cgcctgggcg cacccgcgct ggcgggcaac
     2101 gccgacaacc cgaatagctg gttcgccggg ttcatgcggg gcgtggagtc ccggaaccac
     2161 cgggtggact tcgtcccggt gcactggtat ctcgctcccg gccttcgtga gtcctactcc
     2221 ctcgaccggg ccgtcgacga cctcgcggga cacatcaccc gagtccacga cacctatggg
     2281 ctgcccatct gggtctccga gttcagcctc atcacgtggt accccgcccg ctccgacgcc
     2341 atgccgccgg aggaccaggc ggagttcctc gctgcagcag cggagatgat gacgtcactc
     2401 gcctatgtcg agcgctgggc ttggttctcc ctcacgcctc ccccgtatgc cccggccgtc
     2461 ggcgcgtacg acgacagcgg ccagatcacg ccggtcggac ggcagatccg acagttgacc
     2521 tgacggtcag caaggcgttc gcgccgtcac tcggccaccc tgccccacct tcccgccagc
     2581 gactgcagcc gaccctggtc atggacaccg cgacgcagtc cctagcgtgc acccgtgcct
     2641 ggtccggcac ggcactgcga cgcagggagt ggctccggcc cggaggacca gctcgaaggc
     2701 ctcgggaatg gtggacgctg gcgtgcttga agaccgggtt cgctctccgg caggccgtca
     2761 cagccgacga aaaacgcatc ggccacgccg cggttcttga tctcggtgag cacgagcatc
     2821 gctgaacgtt cgccgaacgg gatgaactcc tcctgcgcgg cccgccacag tcgagggatg
     2881 gatgggtaac ggtttgcgcc actcgtcctc gagctcctcg aatgcggccc aggctgcttc
     2941 ggcagtggcg gcggtacgga acgctttcat gtcgcgggcg gggtggtccc agtacctgcg
     3001 ggaggcgtac cggaaggaag gcggcccgga tcaggtggat cacgaaggaa tagatgccgg
     3061 aaacaccgac atcggacgac cctgttcggc tgctgctgtt gacggacgcg tcggttgagg
     3121 acgaaggcgt cgcccagggt gatgacgat
//
LOCUS       DAOMZY010000386         3147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_30127_length_3147_cov_6.157180, whole genome shotgun sequence.
ACCESSION   DAOMZY010000386 DAOMZY010000000
VERSION     DAOMZY010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30127_length_3147_cov_6.157180"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /gene="pdxY"
                     /locus_tag="PLK64_15790"
     CDS             <1..538
                     /gene="pdxY"
                     /locus_tag="PLK64_15790"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HPV81256.1"
                     /translation="AVYCCDPVMGDVGRGFFVREGIPEFLRDSVVPSADVVTPNQFEL
                     EFLAGRTISTEADLLDAVAAVRATGPSTVLVTSVLTEQTPAGSIQMACVSDDGAWIVT
                     TPMLDMAVRGGGDATAAIFLAHFLTEGPRVALSRTAASMYAVLEATHKGGFDEMRLIA
                     EQDSIAHPVEVFEVRSLG"
     gene            639..1775
                     /locus_tag="PLK64_15795"
     CDS             639..1775
                     /locus_tag="PLK64_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81257.1"
                     /translation="MPIVFVHGVAVRDPDDPQYAAAQRLTRGADWTVVEAALREHVAP
                     AVRPSAPERVALIRVYWGDLGAQPTEVVEGPPLTLREVESPAALTPDELGEALQRMLF
                     EKLPPAQWPAVVDAVWATVADPAIRAQLAARSPKRQASWLVKQVRARLVETAPELSRA
                     FGQAGADLAAAGRRNIRWAMGGVRRPLEELVPVFVGDVLRYLDGRGVPGAPGPIVQRV
                     LAGLAEARDAECGAGEPLVVLTHSMGGQLVYDALTAYADEVPGGMPRVDLWCASASQI
                     GLFAELGMFVAEGPPSVVGVPADRLGYLWNAWSSSDVLSFRAEGWVAGAHDTDFTFSG
                     SLGANHMSYLTDPTFYRTLAAKVRAHCRSAPARHGLAQYGEISD"
     gene            1803..3086
                     /locus_tag="PLK64_15800"
     CDS             1803..3086
                     /locus_tag="PLK64_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPV81258.1"
                     /translation="MDPIRNPYAPGAGQRPPELAGRDEQLHAFDVTLERVSRGRPERS
                     VVLTGLRGVGKTVLLNALRSAAVRAHWGTGKLEARPDQPLRRPLSAALHMAVRELGHP
                     QPDEVAQVLGVIKSFAQRESTESSGGRGAGGAGARGSAGASRLRDRWSVGIDVPAVSG
                     RADSGDIEIDLVELLSDVGGLAADRGVGVAIFIDEMQDLGADDVSALCAAAHEISQSG
                     LPVVIVGAGLPHLPAVLSASKSYSERLFRYSRIDRLDADAAEIALRAPAAEEGASWSS
                     DALGEMYAVTAGYPYFVQAYGKAVWDAAVGPEITVDDVRMAAPEAEAELAVGFFGSRY
                     ERATPAEREYLRAMADAAYRNGVVSEGGGASSASAELDAVPTAEVAALLGKRPQSLSP
                     ARDSLMKKGLVYSSERGQIAFTVPHFGRYLRAHPE"
BASE COUNT          415 a         1014 c         1209 g          509 t
ORIGIN      
        1 cgcggtctac tgctgcgacc ccgtcatggg cgatgtcggg cgcggcttct tcgtgcgcga
       61 gggcatcccg gagttcctgc gcgactcggt cgtgccgagt gccgacgtgg tgacgcccaa
      121 ccagttcgag ttggaattcc ttgccgggcg gacgatttcg actgaggccg acctcctcga
      181 cgcggtcgcc gcggtgcgcg cgaccggccc ctcgacggtt ctcgtgacga gcgtgctgac
      241 cgagcagacc cccgccgggt cgatccagat ggcctgcgtc agcgacgacg gagcctggat
      301 cgtcaccacg ccgatgctcg acatggcggt ccgcggcggc ggcgatgcca cggccgcgat
      361 cttcctcgcg cacttcctca ccgagggacc acgagttgcg ttgtcccgca ccgcagccag
      421 catgtatgcc gtgctcgaag ccacccacaa gggcggcttc gacgagatgc gcctcattgc
      481 ggagcaagac tcgattgccc accctgtcga ggtcttcgag gtccgcagcc tgggctgacc
      541 ggcccacgag tcccgtgcgg gcgcgaaggt gacaggcccc cgcaaggtga gcgggcggca
      601 taccgtggag gcagtcctta gcagacgaaa ggtgagcgat gccgatcgtg ttcgtccacg
      661 gcgtggccgt gcgagacccc gacgacccgc agtatgccgc cgcgcaacgc ctgacccgcg
      721 gggccgactg gaccgttgtc gaggccgcgt tgcgtgagca tgtcgcgcct gcggtgcggc
      781 cctcggcgcc ggagcgggtc gcgctcattc gggtctattg gggtgacctc ggggcgcagc
      841 cgaccgaggt ggttgaggga ccgccgctga ccctgcgcga ggtcgagtcg cccgcggcac
      901 tgacgccgga tgagctcgga gaagcgttgc agcgcatgct tttcgagaag ctgccgccgg
      961 cccagtggcc cgcggttgtc gacgccgtgt gggcgaccgt ggccgacccg gcgatccgcg
     1021 cacaactggc cgcgcggtcg ccgaagcggc aggcgtcgtg gctggtgaag caggtccgcg
     1081 cgcggctcgt cgagacggcc cccgagctct cgcgcgcgtt cggccaggcg ggcgccgacc
     1141 tcgccgcggc cggtcggcgc aacatccggt gggcgatggg tggggtgcgt cggccgctgg
     1201 aggagttggt gccggtcttc gtcggcgacg tgctgcgcta cctcgacggg cgtggggtgc
     1261 cgggggcgcc tgggccgatc gtgcagcggg tgctggcggg gttggccgag gcgcgcgacg
     1321 ccgagtgcgg cgccggcgag ccgctcgtcg tgctgacgca cagcatgggc gggcagttgg
     1381 tctacgacgc actgaccgcc tacgcggacg aggtccctgg aggtatgccg cgcgtcgacc
     1441 tctggtgcgc gagtgcgtcg cagatcggct tgttcgcgga gttggggatg tttgtggccg
     1501 aagggccgcc gtcggtggtg ggggtgcctg ccgatcggtt gggctatctg tggaacgcct
     1561 ggagctcgtc ggacgtgttg agtttccgcg cggagggctg ggtggctggg gcgcacgaca
     1621 cggacttcac cttctccggg tcgctcgggg ccaaccacat gtcctacctg accgatccga
     1681 ccttctatcg gaccttggcg gcgaaggtcc gggcgcactg tcgatcggca ccggcgcgac
     1741 acggactagc gcagtatggg gaaatctcgg actgacgcta gacagcggca tacagtccga
     1801 tcatggaccc gatccgcaac ccctatgcgc ctggggctgg gcagcggccg cccgagttgg
     1861 ccggccgcga cgagcagttg cacgccttcg acgtgacgct cgagcgagtc tctcgcgggc
     1921 gccccgagcg ctcggtcgtg ctgaccgggc tgcgcggtgt cggcaagacc gtgctgctca
     1981 acgcgctgcg atcggcggcc gtgcgggcgc attgggggac cggcaaactc gaagctcggc
     2041 cggaccaacc gctacggcgc ccgctgtcgg cggcgttgca catggcggtc cgcgagttgg
     2101 gccatccgca gccggacgag gtcgcccagg tgctcggggt gatcaagtcg ttcgcccagc
     2161 gtgagtcgac tgagtccagt ggcggtcgcg gcgctggcgg ggccggtgcg cgggggagcg
     2221 cgggggccag tcgcctgcgc gaccgttgga gcgttggcat cgacgtgccc gcggtgtcgg
     2281 gtcgagccga ctcgggcgac atcgagatcg acctcgtcga gttgctcagc gacgtcggcg
     2341 ggctcgcggc cgaccggggc gtgggggtgg cgatcttcat cgacgagatg caggacctcg
     2401 gcgctgacga tgtgtcggca ttgtgcgcag cggcccacga gatctcccag tcggggctgc
     2461 cggtggtcat cgtgggggcg gggttgccgc acctaccggc agtgctgtca gcgtcaaagt
     2521 catactcgga gaggctgttt cgctactccc gcattgaccg gctcgacgcg gacgctgcgg
     2581 agatcgcgct gcgcgcaccg gctgccgagg aaggcgcctc ctggtcgtcg gacgccttgg
     2641 gggagatgta tgccgtgacc gccggctatc cctacttcgt gcaggcctat ggcaaggccg
     2701 tgtgggacgc ggccgtcggg ccggagatca ctgtcgatga cgtgcggatg gcggcgccgg
     2761 aggcggaggc cgagttggcg gtgggcttct tcgggtcgcg gtatgagcgt gctaccccgg
     2821 cggagcggga gtacctgcga gcgatggcgg acgcggcata ccggaatggg gtggtgagcg
     2881 agggtggcgg tgcgtctagc gcgtcggccg agttggatgc cgtgccgacc gctgaggtgg
     2941 ccgcgctgct gggcaagcgg ccgcagtcgc tgtcgccggc gcgggactcg ctgatgaaga
     3001 aggggctggt ctattccagc gagcgcggcc agatcgcctt caccgtcccc cacttcggcc
     3061 gctacctccg cgcccacccc gagtagctgt cgcgccctga cctcgccgca gttgccttgc
     3121 cggagaagga gcgcggcagc tgcggcg
//
LOCUS       DAOMZY010000387         3112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_30617_length_3112_cov_6.494275, whole genome shotgun sequence.
ACCESSION   DAOMZY010000387 DAOMZY010000000
VERSION     DAOMZY010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30617_length_3112_cov_6.494275"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..1689
                     /gene="purB"
                     /locus_tag="PLK64_15805"
     CDS             280..1689
                     /gene="purB"
                     /locus_tag="PLK64_15805"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPV81259.1"
                     /translation="MPSLGDVEPPIALGALDGRYRAAVAPLVDHLSEAALNRERVRVE
                     IEWLIHLTSYAVVPGTRALTGTEHEALRAIVTNFGAAEIAALADIERVTVHDVKAVEY
                     FLKERLAAIAPDPADAGLGELIHFACTSEDVNNLAYALMVKGAVEQVWLPRATALVDQ
                     LATMAREHAATPLLAHTHGQPATPTTLGKELAVMVYRLRRQLERIGRTAYLGKLNGAT
                     GTYGAHLAAVPDHDWQATSRGFVESLGLQWNPLTTQIESHDWQAELYADVARFNRILH
                     GLAQDMWTYISMGYFAQVRGQGTVGSSTMPHKVNPIRFENAEANLEVSTALLDVLAAT
                     LVESRMQRDLTDSSMQRNIGTAFGHSTLAIDNVARGLAGLDAVPERMAADLDANWEVL
                     AEPIQSAMRALTARGVPGMDNPYERLKELTRGRRIGASELREFVAGLGLPADVEARLQ
                     ALTPATYVGIAPELVDHLG"
     gene            complement(1787..2422)
                     /locus_tag="PLK64_15810"
     CDS             complement(1787..2422)
                     /locus_tag="PLK64_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPV81260.1"
                     /translation="MRVDHVSYAAEHDGLHATAERLAKLIGVKPVDGGVHPRFGTRNV
                     ILPLANERYVEVVEVLDHPASDKAPFGQAVRARSANGGGWLGWVVRVDGIEEIGSRLG
                     REPADGYRHTPDGRELRWRQLGVSGLICDPQLPFYVEWAEGTRHPSTDGATQVGLAGL
                     TIAGDPQRVRAWLGLPEDYKPSTLEFTFVAPHGNPGLLAVTFGTPDGPVTV"
     gene            complement(2505..>3112)
                     /locus_tag="PLK64_15815"
     CDS             complement(2505..>3112)
                     /locus_tag="PLK64_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HPV81261.1"
                     /translation="PEFVSAARAAGSEIVTLSRVAFEKVAYREGPDGLLGVLPAVDRP
                     LSGLTLPVDPLVLLVEGVEKPGNLGAMLRTADAAGVDAVVAADPVTDWGNPNVVRASK
                     GTVYAVPVASASTAETLAWCRARGIRLVVTTPETDVLYSDISLSGAVAIAVGAEKHGA
                     SEELLAAADVRVRIPMSGRANSLNVAASAAIVLFEAVRQRR"
BASE COUNT          553 a         1137 c          994 g          428 t
ORIGIN      
        1 cttcaccggc gacttccgca tcaacggcgg accctggacc caaatccccg acacagtgac
       61 ggtcaccggg cccaccacca ccatcaccgt ccacactgcc aaacccgtcc tcgtccacta
      121 accaacaccc tcacccccgc ccattcgagg tatctcgcca tcatcaggta acagccgaat
      181 acctcgaccc ggcaggagac ccggcaggag acccggcagg agacctggca aggcggacgg
      241 ggcgggaggg cggcataccg gatctgggaa gatgaccaca tgccttccct cggtgacgtc
      301 gaacccccga tcgctcttgg cgccctcgac gggcgctacc gcgcggcggt ggctccgctg
      361 gtcgatcacc tctccgaggc ggcgctcaac cgcgagcgcg tgcgcgtcga gatcgagtgg
      421 ctcatccacc tcacctcgta tgccgtcgtc cccggcaccc gcgccctcac cggcactgag
      481 cacgaggcct tgcgcgccat cgtcacaaac ttcggagcgg ccgaaatcgc cgccttggcc
      541 gacatcgagc gagtgacggt ccacgacgtc aaggccgtcg aatacttcct caaggaacgg
      601 ctcgccgcca tcgcccctga cccggccgac gcagggctcg gcgagttgat ccacttcgcc
      661 tgcacgagcg aggacgtcaa caacctcgct tacgcactca tggtcaaagg ggccgtcgag
      721 caggtctggt tgccccgcgc cacggcgctc gtcgaccaac tcgcgaccat ggctcgcgag
      781 cacgccgcca cgcccctcct cgcgcacact cacggccagc cggccacgcc gacgacgctc
      841 ggcaaggaac tcgcggtcat ggtctaccgc ctgcgccgcc aactcgagcg catcgggcgc
      901 acggcatacc tcggcaagct caacggcgcg acggggacct acggcgcgca cctggccgcg
      961 gtccccgacc acgactggca ggcgacgtca cgcggcttcg tggaaagcct tgggctgcaa
     1021 tggaacccgc tgacgaccca gattgagagc cacgactggc aggccgagct ctatgccgac
     1081 gtcgcgcggt tcaaccggat cctgcacggc ttggcgcagg acatgtggac ctacatctcg
     1141 atgggctact ttgcccaggt ccggggccag gggacggtcg gatcgtcgac tatgccgcac
     1201 aaggtcaacc ccatccgttt cgagaacgcc gaggccaacc tcgaggtctc cacagccctg
     1261 ctcgatgtcc tggccgccac cctcgtcgag agccggatgc agcgcgacct caccgactcg
     1321 tcgatgcagc gcaacatcgg aacggccttc gggcactcga cgctcgcgat cgacaacgtc
     1381 gcccgcgggc tggccgggct cgacgccgtg ccggagcgga tggcggcgga tctcgacgcc
     1441 aactgggagg tgcttgccga gccgattcag tcggcgatgc gggcgctgac cgcgcggggc
     1501 gtgccgggga tggataaccc ctacgagcgg ctcaaggagc tcacccgggg gcgccggatc
     1561 ggggcgagcg agttgcgcga attcgttgcg ggactaggcc tgccggccga cgtcgaggcc
     1621 cgcctgcagg cgctgacccc cgcgacctac gtaggcatcg cgccggagtt ggtcgaccac
     1681 ctcggctgac gcatgctcct cgacgaacga tcgtcaggag cccgcctcac caactcgcca
     1741 tgcacgggac cagaccgcgc cggcaaaccc accaaagagg cggggctcag acggtgacgg
     1801 ggccgtcggg cgtgccgaac gtgaccgcga gaagacccgg gttgccgtgc ggcgcaacga
     1861 aagtgaactc caacgtcgac ggcttgtagt cctcgggaag ccccagccag gcccggaccc
     1921 gctgcgggtc gccggcgatg gtcagcccgg ccaacccgac ctgcgtggcg ccatcggtcg
     1981 acgggtggcg ggtgccttcg gcccactcga cgtagaaggg gagctgtggg tcgcagatga
     2041 gcccgctgac gcccagctgg cgccagcgca actcacgacc gtcaggggta tgccggtatc
     2101 cgtcggcggg ctcgcgaccc aaccggctgc cgatctcctc gatgccatcg acccggacca
     2161 cccagccgag ccagccgcca ccgttggcgg accgggcgcg gaccgcctga ccgaaggggg
     2221 ccttgtcgga agcggggtgg tcaagcacct cgacgacctc gacgtagcgt tcgttggcga
     2281 ggggcaggat gacgttgcga gtgccgaacc gagggtggac gcccccgtcg accggcttca
     2341 ctccgatcag cttggccaac cgctcagcgg tggcatgaag gccgtcgtgc tccgccgcgt
     2401 aggagacgtg gtcaacgcgc atgccgccca tcgtgacaca ggtttgaggg tgcttttgac
     2461 cactgtcggc cccggcgccg ggggccaccg caatgtaagc gctactacct tcgctgccgc
     2521 actgcttcga acagaacgat cgccgctgac gctgcaacgt tcaacgaatt ggccctgcca
     2581 gacatgggaa ttcgcacgcg aacgtccgcc gccgcgagca attcttcgga tgcgccgtgc
     2641 ttctccgcac cgaccgcgat cgcgaccgcc ccgctcaaag agatgtccga atagaggaca
     2701 tccgtctcag gggttgtcac cacgaggcgt atgccgcgcg ccctgcacca cgccaacgtc
     2761 tcggccgtcg acgcggaggc gacgggcacg gcatacacgg tgcccttgct ggcccgcacg
     2821 acgttggggt tgccccagtc ggtcaccgga tccgccgcca caacggcgtc gaccccggcc
     2881 gcatcggcgg tgcggagcat cgccccgagg ttcccaggct tctcgacacc ctcgacgagc
     2941 agcaccagcg ggtcaacggg aagggtcaaa cccgagagcg gccgatcgac cgccggaagc
     3001 accccgagga gaccatcggg cccttcgcga taggcgacct tctcgaaggc cacccgcgac
     3061 aaggtgacga tctccgaccc cgccgcccgg gccgccgaga cgaactcagg ct
//
LOCUS       DAOMZY010000388         3109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_30666_length_3109_cov_5.567453, whole genome shotgun sequence.
ACCESSION   DAOMZY010000388 DAOMZY010000000
VERSION     DAOMZY010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30666_length_3109_cov_5.567453"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..2860
                     /gene="gyrA"
                     /locus_tag="PLK64_15820"
     CDS             200..2860
                     /gene="gyrA"
                     /locus_tag="PLK64_15820"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013490894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPV81262.1"
                     /translation="MSEQPPIEPPDDEETTPVEEPAHELGTTPPPHTGGDKIEPIDLN
                     TEMQRSYIDYAMAVIVSRALPDVRDGLKPVHRRVLYAMYDGGYRPDRGYNKCSRVVGD
                     VMGQYHPHGDSAIYDALVRLVQPWSLRYPLVDGQGNFGSPGNDGAAAPRYTECRMAQL
                     SMEMVRDIEQDTVDKKPNYDGKTEEPVVLPARFPNLLVNGSSGIAVGMATNIPPHNLR
                     EVASGAQWYLSNPTAGREELLAQLMTMVKGPDFPTGALIMGRKGIEDAYRTGRGSIIM
                     RAAVAVEEIQNRVCLVVTELPYQVNPDMLAQKIAELVREGRLAGIADIRDETSGRTGQ
                     RLVIVLKRDAVAKVVLNNLYKHTQLQTTFGANMLALVDEVPRTLPLDAFIRHWVDHQI
                     DVIVRRTAYRLRKAEAEIHILRGYLKALDQLDAVIALIRASATVDIARTGLIELLDID
                     EVQANAILALQLRRLAALERQKIIDDHNEIEARILDLQDILAKPERQRSIVSTELAEI
                     VDKFGDDRRTRIEFFEGDMSVEDLIPEEDVVVTITRGGYAKRTKTDLYRSQRRGGKGV
                     RGASLRGEDVVEHFFTTTTHHWLLFFTNLGRVYRAKGYELPEGGRDAKGQHVANLMAF
                     QPGEEIAQVLAITDYQQAPYLVLATRAGLVKKTRLSEYDSPRTGGLIAVNLRDDDVLV
                     GAGLAAAEDDLLLVSRKGQSVRFRADDEHLRPMGRATSGVTGMRFRDGDRLLAMSVVQ
                     AGTEPDVFVVFENGLAKRTPIAEYRLQGRGGTGIQVAKLSDKGGELVGALTVTAEDEV
                     MVVMEKGKIVRSRVDEVRHTGRSTMGVKFATPDRGDAIVAVARNAEGSSEDDNGVPSD
                     ENIPAEGPGDESVAPAEADASTDEQPGGSE"
     gene            2857..>3109
                     /locus_tag="PLK64_15825"
     CDS             2857..>3109
                     /locus_tag="PLK64_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81263.1"
                     /translation="MSTPGGTPKPTSPTPASGAAVTTATSAAGPAAAPSTAATVVTKA
                     VTDATAPRTRPNPAVSPSTSPSSSPSSNGGVGTGAGGGAG"
BASE COUNT          526 a         1032 c         1086 g          465 t
ORIGIN      
        1 atctacacgg cattctacac tcttcccctg ctgcctcccg tctctccgtc accgccgccc
       61 tggcgcggca tacctctgcc ggctcccgct gcgccccgcc gcccccggtt tgcggagtgt
      121 tagccacatc ccggtgtggg caacactccg tgaaggcgcg gcatacccct tggatgtcgt
      181 ttaaccgtga ggaaacccga tgagtgagca gccgccgatc gagccccccg acgacgagga
      241 gaccaccccc gtcgaggagc ccgcgcacga gctcggcacc accccgccgc cgcacaccgg
      301 cggcgacaag atcgagccga tcgatctcaa caccgagatg cagcggtcgt atatcgacta
      361 tgcgatggcg gtcatcgtca gtcgggcgct gccggacgtg cgcgatgggc tcaagccggt
      421 gcaccggagg gtgctgtatg cgatgtatga cgggggctac cggcccgacc gtgggtacaa
      481 caagtgcagc cgggttgtcg gcgacgtcat ggggcagtac cacccgcacg gtgactcggc
      541 gatctatgac gcgttggtgc ggctcgtgca gccgtggtcg ctgcggtatc ccttggtcga
      601 cggccagggc aacttcggca gcccgggcaa cgatggggcg gcggcgccgc gatacaccga
      661 atgccggatg gcgcagttgt cgatggagat ggtccgcgac atcgagcagg acaccgtcga
      721 caagaagccg aactatgacg gcaagacgga ggagccggtc gtcctgccgg cccgcttccc
      781 caacctgctg gtcaacggct cgtcgggcat cgccgtcggc atggcgacga acatcccgcc
      841 gcacaacctg cgcgaggtgg cctcgggggc gcagtggtat ctcagcaacc cgacggccgg
      901 gcgtgaggag ttgctcgcgc agctcatgac gatggtcaag gggcccgact tcccgaccgg
      961 cgcgctgatc atggggcgca agggcatcga ggacgcctac cggaccgggc gcgggtcgat
     1021 catcatgcgc gcggccgtgg cggtcgagga gatccagaac cgggtgtgcc tggtcgtcac
     1081 cgagctgccg tatcaggtca accccgacat gctggcgcag aagatcgccg agctggtgcg
     1141 cgaggggcgc ctggccggca ttgccgacat ccgcgacgag acgtccgggc gcaccgggca
     1201 gcggctggtc atcgtgctca agcgcgacgc cgtcgccaag gttgtcctca acaacctcta
     1261 caagcacacg cagctgcaga cgaccttcgg cgccaacatg ctggcgctgg tcgacgaggt
     1321 gccgcgaacg ctgccgctgg atgccttcat tcggcactgg gtcgaccacc agatcgacgt
     1381 gatcgtgagg cgcacggcat accggctgcg caaggccgag gccgagatcc acatcttgcg
     1441 cgggtacctc aaggcactgg accagctcga tgccgtgatc gcgttgatcc gcgcgtcggc
     1501 gaccgttgac atcgcgcgca ccgggttgat cgagttgctc gacatcgatg aggtgcaggc
     1561 caacgcgatc ctggcgttgc agctgcgccg gctggccgcg ttggagcggc agaagatcat
     1621 cgacgaccac aacgagatcg aggcgcggat cctggatttg caggacatcc tcgccaagcc
     1681 cgagcggcag cggtcgatcg tgtcgaccga gctcgccgag atcgtcgaca agttcggtga
     1741 cgaccggcgg acgcggatcg agttcttcga gggcgacatg tccgtcgagg acctcatccc
     1801 cgaggaggac gtcgtcgtga cgatcacccg gggtggttat gccaagcgga ccaagaccga
     1861 cctctatcgg tcgcagcggc gtggcggcaa gggcgtgcgt ggggcatcgc tgcgtggcga
     1921 ggatgtcgtc gagcacttct tcacgacgac aacgcatcac tggctgctct tcttcaccaa
     1981 cctcggccgg gtctatcggg ccaaggggta tgagctgccc gagggtgggc gcgacgccaa
     2041 gggtcagcac gtggccaacc tgatggcctt ccagccgggc gaggagatcg cccaggtgtt
     2101 ggcgatcacc gactaccagc aggcgcccta tctcgtgttg gcgactcgag ccggcctggt
     2161 caagaagacc cgcctgtccg aatacgactc gccgcgcacg ggtggcctca tcgcggtcaa
     2221 cctgcgcgac gacgacgtgc tcgtcggggc gggactcgcg gctgccgagg acgacctgct
     2281 cctggtgtcg cgcaagggtc agtcggtgcg gttccgggcc gacgacgagc acctgcggcc
     2341 gatgggtcgg gcaacgtccg gcgtcaccgg catgcggttc cgcgacggcg accggctgct
     2401 ggcgatgtcg gtcgtgcagg ccgggaccga gccggatgtc ttcgtcgtct tcgagaacgg
     2461 cctggccaag cggaccccca tcgccgagta tcggctgcag ggtcggggcg gcaccggcat
     2521 ccaggtggcc aagctgtccg acaagggtgg cgagctcgtc ggcgccttga cggtgaccgc
     2581 cgaggacgag gtcatggtcg tcatggagaa gggcaagatc gtccgctccc gcgtggacga
     2641 agtgcggcat accggtcgct cgacgatggg cgtcaagttc gcgacgcctg accgcgggga
     2701 tgcgattgtg gccgtcgcgc gcaacgccga gggcagctcc gaggacgaca atggcgtacc
     2761 gtctgacgag aacatccctg ccgaggggcc gggagacgag tctgtagcgc ctgccgaggc
     2821 ggacgcgagc accgacgagc agcctggagg tagcgagtga gcacacccgg gggcactccc
     2881 aagcccacct cgccgacccc tgcttccggc gcggccgtga cgacggcgac gtccgccgca
     2941 gggcccgccg cggcaccgtc gaccgcagcc acggtggtca cgaaggctgt cactgacgcc
     3001 actgctccac ggacccggcc caacccggcc gtcagcccgt cgaccagccc ttcctctagc
     3061 ccgtcgtcca acggcggggt cggcacgggg gcagggggcg gggcgggcg
//
LOCUS       DAOMZY010000389         3108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_30678_length_3108_cov_5.679004, whole genome shotgun sequence.
ACCESSION   DAOMZY010000389 DAOMZY010000000
VERSION     DAOMZY010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30678_length_3108_cov_5.679004"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PLK64_15830"
     CDS             <1..614
                     /locus_tag="PLK64_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPV81264.1"
                     /translation="LCFYAALSTGTMGVVAPIAAVGSGIPVLLGVLSGEKPSALAWWG
                     IAVAIAGVVLASGPEMKEGLGARPVLLACLAAVGFGVALFALDRGARVSLLHTLWGMR
                     LTSAIGYAALALAARSLGGVARRQVPVLAAVGAGDLAANALFGMASSMGMVSVAAVLG
                     SLYPVMTILLARYFLNERLRRIQVIGSTLALCGVVLIAAGGRG"
     gene            complement(643..1719)
                     /locus_tag="PLK64_15835"
     CDS             complement(643..1719)
                     /locus_tag="PLK64_15835"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HPV81265.1"
                     /translation="MFSYAVSRRLRPTALTVAALLALGACSSGSSPTSTNAAREGGVA
                     VAPAPAPGAAVGAAAAADTKLSAPDVASVGEAALVDTKIIRTASIYLTVTSVETAAAA
                     VRGVAAGLGGAVASETVNATEVAPSADPAIGGKATAPVRRVGNYGQLVLSIPTDRLDA
                     ALDQLSKLGTVVQRASASDDVTAAYVDTESRIATKKASIERVRALMSQTQNISQIVEL
                     ESQLAQREADLESLQAQLASMTKRIAMATVTVTVSSNPEVIPPVEQDGFMAGLRSGWN
                     AFLASLTVGLTVLGALLPWLVVLALIAIPVRSWLRRRTERQEATPTTSPSWAASTPAA
                     PVREPEPAAATAGPAASEGTSEKA"
     gene            complement(1829..2293)
                     /gene="msrB"
                     /locus_tag="PLK64_15840"
     CDS             complement(1829..2293)
                     /gene="msrB"
                     /locus_tag="PLK64_15840"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013349322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HPV81266.1"
                     /translation="MTDPTSTQSVPPADAVPTPGRYAVQLTDTQWRAKLSAAEFHVLR
                     EAGTERPFTGEYTDTKTDGVYSCRACGAELFRSESKFDSHCGWPSFFEPLAGDRVEYI
                     EDRSIPGRPRVEVRCAGCGSHLGHVFEGEGYGTPTDQRYCINSVCLTLEPRT"
     gene            2464..>3108
                     /locus_tag="PLK64_15845"
     CDS             2464..>3108
                     /locus_tag="PLK64_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HPV81267.1"
                     /translation="MADAQVECPTPDGGTRAVRISSPDRVLWPQDGITKLDLAHYIRD
                     VGPAMLRRVGGRPMTLQRFPTGIEGEEFYSKNPPKGLPAHVRTVLMTYPSGRKHPQLV
                     VDEIATAVWGVQMNTVTFHPWPVRATDAEGALDRPDEVRIDLDPQPGCGFREAVEAAY
                     VLREVLAEAGLTGFPKTSGNRGVHVFCPIEPAREFLDVRHGVIAIARELERRMPD"
BASE COUNT          419 a         1033 c         1148 g          508 t
ORIGIN      
        1 tgttgtgttt ctatgccgcg ctctccacgg ggaccatggg tgtcgtcgct cccattgccg
       61 ccgtcggctc gggcatcccc gtgttgctcg gggtgctcag cggcgagaag ccctccgcct
      121 tggcctggtg ggggatcgcc gtcgccatcg ccggagtggt gctggcgagc gggccggaga
      181 tgaaggaggg cctgggcgcc cgacccgtgt tgttggcgtg tctggccgcg gtcggcttcg
      241 gcgtcgctct gttcgccctc gaccgcggcg cgcgggtgtc gctgctgcac accttgtggg
      301 ggatgcgcct caccagcgcc atcgggtatg ccgcgctggc gctcgccgcg cgctccctcg
      361 gtggggtcgc ccgccgccag gtgccggtgc tggcggccgt cggcgcaggt gatcttgccg
      421 ccaacgcgct cttcgggatg gcctcgtcga tgggcatggt cagcgtcgcc gccgtcctcg
      481 gatcgctcta ccccgtgatg acgatcctgc tggcccgcta cttcctgaac gaacggctgc
      541 gccggatcca ggtgatcggc tcgaccttgg ccctctgcgg agtcgttctg atcgccgcag
      601 gcgggcgggg ctgaccccgc ccgccacggc acgtcggctg attcaggcct tctcgctcgt
      661 gccctcggag gccgccggtc cggcggtggc tgctgccggc tccggctcgc ggacgggcgc
      721 tgcgggtgtc gaggcagccc atgacggtga cgtggtgggt gtcgcctcct gacgctcggt
      781 gcgccgacgc agccaggacc ggaccgggat cgcaatgagg gccagaacga cgagccaggg
      841 gagcagggcc cccaggacgg tcaggcccac cgtgaggctg gccaagaagg cgttccagcc
      901 tgaccgcaga cctgccatga agccgtcctg ctcgaccggt gggatcactt cggggttgga
      961 gctcaccgtc acggtgacgg tcgccatcgc gattcgtttc gtcatcgacg ccagctgcgc
     1021 ctgcaacgac tcaaggtcgg cttcgcgctg ggccagttgg ctctccaact cgacgatctg
     1081 gctgatgttc tgggtctggc tcatcagcgc tcgcacccgc tcgatgctcg ccttcttcgt
     1141 cgcgatgcgc gactcggtgt cgacgtatgc cgcggtgacg tcatcgctcg cgctggctcg
     1201 ctgcacgacg gtgccgagtt tcgagagttg atccaaggcg gcgtcgagcc ggtcggtcgg
     1261 gatcgacagc accaactggc cgtagttgcc gacccgccgc accggcgcgg ttgccttccc
     1321 accgatcgcg ggatcggcgc tcggtgccac ttcggttgcg ttgaccgtct cgctggcgac
     1381 cgctccgccc aagccggccg caacgccacg cacggcggct gcggcagtct cgacggaggt
     1441 gacggtgaga tagatgctcg cggtgcggat gatcttggtg tccacgagtg ctgcttcgcc
     1501 cactgacgcg acgtcaggag ccgacagttt ggtgtcggcc gccgcagcgg caccgacagc
     1561 ggcgccgggc gccggagccg gcgcaacggc cactccgccc tcgcgggcgg cgttcgtgct
     1621 ggtcggtgac gacccgctgg agcaggcccc gagggcgagg agcgcggcaa cggtgagagc
     1681 ggttggtcgc aaccggcggg acacggcata cgaaaacatc ggggtcctcc tcgggcgggc
     1741 cggacggggc cggcgatgga gcgttcggcc cttggagggg gagccgcccg ggtgtggttc
     1801 ccaggcgcgc gtctcgatca ggtctcgatc aggtgcgagg ttccaaggtc aggcagaccg
     1861 agttgatgca gtagcgctga tcggtgggcg tcccatagcc ctcgccctcg aagacgtgcc
     1921 ccaggtggga gccgcagccg gcgcaccgga cctcgacgcg gggccgaccg gggatcgagc
     1981 ggtcctcgat gtattcgacg cggtccccgg ccagcggctc gaagaacgac ggccacccgc
     2041 agtgcgaatc gaacttcgac tcgctgcgga acagttccgc gccgcaggcc cggcaggagt
     2101 agaccccgtc tgtcttcgtg tcggtgtact cgccggtgaa cgggcgttcc gtgcccgcct
     2161 cgcgcaacac atggaactcg gccgccgaga gcttggcccg ccactgggtg tcggtgagtt
     2221 gcacggcata ccgccccggg gtggggacgg cgtcggcggg cgggacggac tgcgtggagg
     2281 tggggtcggt catggtgggg ataacgcacg aaggccccgg gatcgtcccc ggggccttcg
     2341 tgcgctcgga tccgagccgg tcagatgccg gtgagggctg aactcgaagt cgttgacgtc
     2401 agcggcaacg acgaccacgg ggggagaggc tgcgtgtgcc tggtgcgtgc aatagtgacc
     2461 gacatggccg atgctcaggt ggaatgcccg acccctgacg ggggaacgcg ggcggtgcgg
     2521 atctccagcc cggaccgggt gctgtggccg caggatggca tcaccaaact cgatctggcg
     2581 cactacatcc gggacgtcgg gccggcgatg ctgcgccggg tcggcggtcg cccgatgacg
     2641 ttgcagcgct tcccgaccgg catcgagggg gaggagttct actccaagaa cccgccaaaa
     2701 ggcctgccgg cccacgtccg caccgtgctg atgacctacc cctccgggcg caagcatccc
     2761 cagctcgtcg ttgacgagat cgccacggcg gtctggggcg tgcagatgaa caccgtgacc
     2821 ttccacccgt ggccggtgcg cgccaccgat gccgaggggg cgctggaccg acccgacgag
     2881 gtccgcatcg acctggaccc gcagccgggg tgcggcttcc gcgaggcggt ggaggcggca
     2941 tacgtgctgc gtgaggtgct cgcggaggcg gggctgacgg gcttcccgaa gacgtcgggc
     3001 aaccggggcg tgcatgtctt ctgcccgatc gagcccgcgc gggagttcct cgatgtgcgc
     3061 cacggggtga tcgcgatcgc ccgcgagttg gagcgccgga tgcccgac
//
LOCUS       DAOMZY010000390         3049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_31519_length_3049_cov_3.291917, whole genome shotgun sequence.
ACCESSION   DAOMZY010000390 DAOMZY010000000
VERSION     DAOMZY010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31519_length_3049_cov_3.291917"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PLK64_15850"
     CDS             complement(<1..379)
                     /locus_tag="PLK64_15850"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HPV81268.1"
                     /translation="MSKPKNPTHPPPSLTITDADIRPTTELLWRIHRTSGLHPAAWND
                     FRTYGPLPTMRWDPHPQPTGIHLGRGVLYASIDLRTAVAEVFQSTRRIDPFTGDPHVT
                     TFIPRRPLRLLRLYSQDATWLLNH"
     gene            complement(376..945)
                     /locus_tag="PLK64_15855"
     CDS             complement(376..945)
                     /locus_tag="PLK64_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81269.1"
                     /translation="MLSIAEVFDRYDLELSESDFARELARSLADAPAPTDVSARLLTD
                     LQESGGLTDPARLSTWSQRDDRDLRVAVQSTMLSDLLAATYSASQTAELLSISRSRLS
                     HKTKSVPLLTALVGRHRRYYRWQFSEGHIVPGLGILADHLTGDEHPLDIATMMTSRSD
                     DLDGRTPVDFLVTGGNPVLVARLLEGLSF"
     gene            complement(1067..1450)
                     /locus_tag="PLK64_15860"
     CDS             complement(1067..1450)
                     /locus_tag="PLK64_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81270.1"
                     /translation="MNASDVARAAILPWLAGAVFLTLVQVPFQFYRAWHDPIRAGMEP
                     PILGTVALTGLTFALAGTRPHATSEGGRWIVAALVTLVAIAGVLIIGALTDNSWGPTV
                     WAGLSGYFLFVPVFVGLALRRADKR"
     gene            complement(1494..1799)
                     /locus_tag="PLK64_15865"
     CDS             complement(1494..1799)
                     /locus_tag="PLK64_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81271.1"
                     /translation="MICCQFIFKPGTYDEDFHRLDGQIDEFARALPGFERTETWFSED
                     RSVVNAVYYFADLQSVRELARFPQHQEAKSQVTRWYDGYQIIVSEITATYGDGRLPR"
     gene            complement(1796..2560)
                     /locus_tag="PLK64_15870"
     CDS             complement(1796..2560)
                     /locus_tag="PLK64_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HPV81272.1"
                     /translation="MTTPLHAANDGAHLHAVLTGTYLDGALLDERVANLRPDYRALLI
                     VATGVRGGPSDAASDEALTRAETLAKARLNGAPPESLPEIAAWRDAFLGFGVKPRQAR
                     SSVEALLRRVDAGLPRIDRLTDLYNAISIEHLIPLGGENLDRYVGPPRLVRADGDETF
                     ETVADGIPTTVTVDPGEVVWRDDLGVTCRRWNWRQCVRTRLDPSTTNVLFILDGLDPV
                     GTDRLEAAGAALVDALTRHAPDAAATTRLIGAGSPR"
     gene            complement(2557..>3049)
                     /locus_tag="PLK64_15875"
     CDS             complement(2557..>3049)
                     /locus_tag="PLK64_15875"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HPV81273.1"
                     /translation="VSRRLLVNVEQGSTNPTIGTLLRLSDALGIGLPALVDTSGDPST
                     PVTVRRAADQTPVWTSPSGGSATMVAGTAPPDSCELWDWRLGPGDAHTSEAHRAGTRE
                     LLLVLSGTVDLQVGDTEQRLKTGDSATFDGATPHAHRNASATRPARFTLAVFEPNLSK
                     EHG"
BASE COUNT          434 a          883 c         1238 g          494 t
ORIGIN      
        1 cgtggttcag cagccacgtg gcgtcctggg agtagaggcg caacagccgc aggggccggc
       61 gcgggatgaa cgtcgtcaca tgggggtcgc cggtgaatgg gtcgatgcgg cgcgtcgatt
      121 ggaagacctc ggccaccgcg gtgcgtaggt cgatcgaggc ataaagaact ccccggccga
      181 gatgtatgcc ggtcggctgg gggtgggggt cccaccgcat cgtgggcagc ggaccgtagg
      241 tgcggaaatc gttccaggcg gcgggatgga gcccggaggt tcggtggatg cgccacagca
      301 gttcggtggt gggccggatg tctgcgtcgg tgatcgtgag gctcggaggc gggtgggtcg
      361 ggttcttggg cttgctcaga aggacaaccc ttcgagcagg cgcgcgacga gcaccgggtt
      421 gccgccggtg accaggaagt ccaccggggt gcggccgtcg aggtcgtcgg agcgtgacgt
      481 catcatggtg gcgatgtcca gcgggtgttc gtcgccggtc agatggtcgg ccaggattcc
      541 tagcccgggg acgatgtgtc cctcgctgaa ctgccagcgg tagtagcgac gatgcctgcc
      601 gaccaacgcg gtgagcagcg ggacggactt ggtcttgtgg gacagccgcg agcggctgat
      661 gctgagcagc tccgcggtct gggacgccga gtaggtcgcc gccagcaagt cgctgagcat
      721 ggtcgattgc acggccaccc gcaggtctcg gtcgtcgcgt tggctccacg tgctcagccg
      781 ggccgggtcg gtgagcccgc ccgactcctg caggtcggtc agcagccgcg ccgacacgtc
      841 tgtgggtgcc ggggcgtcag ccagcgagcg ggccaactcc cgggcgaagt cgctctcgct
      901 gagctccagg tcgtaccggt cgaacacttc tgcgatgctc aacatcgtct acctccgacc
      961 cctaggttac acccgagttg cgcttagttg cgggtgcgga tcgtggtggg cgggcggcat
     1021 acccccgcct gcctgctggt gtggctgagc ggcgaccggc ggggtgtcag cgtttgtctg
     1081 cgcggcgcaa ggcgaggcca acaaagacgg ggacgaagag gaagtagccg gacagccctg
     1141 cccagacggt gggtccccag gagttgtccg tcagggcacc gatgatcagg acgcctgcaa
     1201 tggctacgag ggtgacgagg gcggcaacga tccagcgtcc gccctcgctg gtcgcgtgcg
     1261 gccgggttcc ggccagggca aacgtcaggc cggtgagggc gaccgtgccc aggatcggcg
     1321 gctccatccc tgcccggatg gggtcgtgcc aggctcggta aaactgaaat ggcacctgaa
     1381 ccaacgtcag aaagacggct cctgcaagcc aggggaggat ggcggcgcgg gccacgtcgg
     1441 aagcgttcac ccttcgacgt tcgaggccat aggcaagtct cgcaccgcct cgatcagcgg
     1501 gggaggcggc cgtcgccgta ggtggcggtg atctcgctga cgatgatctg gtagccgtcg
     1561 taccagcggg tgacctggct cttggcctcc tggtgttgcg ggaagcgggc gagctcgcgg
     1621 accgattgga ggtcggcgaa atagtagacg gcgttgacga cgctgcggtc ctcggagaac
     1681 caggtctctg tgcgctcgaa tccgggcagt gcgcgcgcga attcgtcgat ctgcccgtcg
     1741 aggcgatgga agtcctcgtc gtaggtcccg ggcttgaaaa tgaattggca gcagatcatc
     1801 gcgggctccc tgcgccgatg aggcgagtgg tggcggcggc gtcgggggcg tgccgggtga
     1861 gggcgtcgac gagtgcggcg ccggcggctt cgaggcggtc ggtgccgacg gggtcgaggc
     1921 cgtcgaggat gaacaggacg ttggtggtgg acgggtccag gcgggtgcgg acgcactgcc
     1981 gccagttcca gcggcggcag gtgaccccga ggtcgtcgcg ccacaccacc tctccggggt
     2041 cgaccgtcac agtggtgggt atgccgtccg cgaccgtttc gaaggtctcg tcaccgtcgg
     2101 cgcggaccag gcggggcggg ccgacgtagc ggtcgaggtt ctccccgccg agggggatga
     2161 ggtgctcgat cgagatggcg ttgtagaggt cggtgagccg gtcgatgcgg gggagcccgg
     2221 cgtcgacgcg gcgaaggagg gcttcgacgc tgctgcgggc ttggcggggt ttgaccccga
     2281 agccgaggaa cgcgtcgcgc caggcggcga tctcggggag cgactcgggg ggcgcgccgt
     2341 tgaggcgggc cttggcgagg gtctcggcgc gggtgagggc ctcgtcgctc gcggcgtcgc
     2401 tggggccgcc gcgcacgccg gtggcgacga tgagcagcgc gcggtaatcg gggcgcaggt
     2461 ttgcgactct ctcgtccagg agcgcgccgt cgaggtaggt gccggtgagg acggcgtgga
     2521 ggtgcgcgcc gtcgttggcg gcgtgcaagg gggtggtcat ccgtgctcct tggagaggtt
     2581 gggctcaaag acggcgagcg tgaatcgtgc cggcctggtg gcggaggcgt tgcggtgggc
     2641 gtggggggtg gcgccgtcga aggtcgccga gtcgccggtc ttgagccgtt gctcggtgtc
     2701 gccgacctgc agatcaaccg tgccggagag gacgaggagc agttctcggg tgccggcgcg
     2761 gtgggcctcg ctggtgtgag cgtcgccggg gccgagccgc cagtcccata gctcgcagct
     2821 gtcgggtggg gcggtgccgg cgaccatcgt ggccgacccg ccgctggggc tggtccacac
     2881 gggggtctgg tctgcggcgc ggcgcacggt gacgggggtg ctcgggtcgc cgctggtgtc
     2941 gaccagtgcc gggaggccga tgccgagggc gtccgagaga cgcagaaggg tgccgatcgt
     3001 ggggttggtg ctgccctgct caacgttgac gagcagcctg cggctgacg
//
LOCUS       DAOMZY010000391         3041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_31630_length_3041_cov_7.526792, whole genome shotgun sequence.
ACCESSION   DAOMZY010000391 DAOMZY010000000
VERSION     DAOMZY010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31630_length_3041_cov_7.526792"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..228)
                     /locus_tag="PLK64_15880"
     CDS             complement(40..228)
                     /locus_tag="PLK64_15880"
                     /inference="COORDINATES: protein motif:HMM:NF025569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPCC family cysteine-rich protein"
                     /protein_id="HPV81274.1"
                     /translation="MCVKLGDHAHGSVQDGPVSFPCPCCGYLTFGGSPGSYEICEICF
                     WEDDAVQLRWPDKPALRT"
     gene            complement(260..646)
                     /locus_tag="PLK64_15885"
     CDS             complement(260..646)
                     /locus_tag="PLK64_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81275.1"
                     /translation="MTHLARTLLAVAVHLLPSQCRSRYDDEFDAELLSLSRRRRLGYA
                     LSILAAAPRLRWALLEVLCGGRASLRCWLGKHHDRRLHPNPDNHEVIALQCRRCGRVR
                     DPKQYLPRKQRLDDVAWGGAFLAGGR"
     gene            complement(643..975)
                     /locus_tag="PLK64_15890"
     CDS             complement(643..975)
                     /locus_tag="PLK64_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPV81276.1"
                     /translation="MTAPTRAVLEVFLADPDTPRYGLEIGSHAVLPSGTVHPILARLE
                     GLGWLDSGWEDVDPSAAGRPRRRFYRLTAAGAAQAEAAIEAADARRSHLRARRPTPLV
                     PGFGPAQS"
     gene            complement(1079..1351)
                     /locus_tag="PLK64_15895"
     CDS             complement(1079..1351)
                     /locus_tag="PLK64_15895"
                     /inference="COORDINATES: protein motif:HMM:NF033691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HPV81277.1"
                     /translation="MDRAYYEEIAGLLHGLLIRLGDRLPSKDVALIAEFIDANELGLA
                     LEQLADVLSEDAQPLSSDERADMLALADRMQIGNRVPRALEFCPAR"
     gene            1552..2766
                     /locus_tag="PLK64_15900"
     CDS             1552..2766
                     /locus_tag="PLK64_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81278.1"
                     /translation="MADVTGQVQLSPDALSRLLDQDAMRAILSPDAKVPGASNPEVQL
                     AEFWEVIDIVRSCAGKVFTSPAEAEAELFGTASGGGFGIRQDLRDAVSGLLVSIDAAP
                     NQGGVAPSGGHATSGGKWLAAATVAQVSERLGNYVYVLVDPRDSRPFYVGKGIGLRMM
                     SHGLEALDAHGDTDGKKVARIKEIRAAGLEHEIWIVRYGLTRSEYTAVEAAVIDTLGS
                     FPVVPSAKAYRPLELREELTNMRREDAKGKGMILLERLVDELAAPPLTTAAPLLLITL
                     SAWTDEQMDVAGGRTRHGNGFKREWYEPATRDMHIHELEMSTTAWWKLSRKSVEARGI
                     HHAVAVHRGVTRGLFAIKTDSWASANGRSGFVGEPVLAGDLYDEVVGPHGHRVTTKQR
                     GTQNPIAYWPRA"
BASE COUNT          544 a          982 c         1024 g          491 t
ORIGIN      
        1 gggccctacc ccgtcaccac gcagggcacc cgtgcccggt caagttcgaa gagccggctt
       61 gtctggccag cgcaactgaa cggcgtcgtc ctcccagaag cagatctcgc agatctcgta
      121 tgagcccggc gagccaccga aggtgaggta gccgcagcat gggcagggga aggacacagg
      181 accatcttgg acggagccgt gcgcgtggtc accaagtttc acgcacatgc cgcgactcgg
      241 gttgggcgcg cggttggcgt cagcgaccgc ccgcgaggaa ggcgccgccc caggcgacgt
      301 cgtcgaggcg ttgcttacgc ggcaggtact gcttggggtc gcgcacccgg ccgcaccgtc
      361 ggcactgcaa ggcgatgacc tcgtggttgt cggggtttgg gtgcaggcgc cggtcgtggt
      421 gcttccccag ccagcaccgc agcgatgccc ggccaccgca gagcacttcc agcaacgccc
      481 atcgaagccg gggagctgct gccaggatcg acaacgcgta tccgagccga cgccggcggg
      541 aaagggacag cagctcggcg tcgaactcgt cgtcgtagcg cgatcgacac tggctcggca
      601 gcaggtgcac cgccaccgcc agaagggtgc gggccaggtg ggtcatgact gcgccggccc
      661 gaagccaggc accaggggcg tcggccgtcg cgccctgagg tgcgagcggc gcgcgtcggc
      721 agcctcgatg gccgcctcgg cctgggccgc gcccgcggcc gtaagccggt agaagcgtcg
      781 tctcggccgg ccggccgccg aggggtcgac atcctcccag ccgctgtcga gccagccgag
      841 cccttcgagc cgcgcgagta tcgggtgcac cgtgccgctg ggcaggacgg cgtgcgaacc
      901 gatctcgagg ccgtagcgcg gggtatccgg gtcggcgagg aacacctcga gcacggcgcg
      961 ggtcggtgca gtcatacgtg ggtggtgcgt gaccatggtc taactctacc tagggcacct
     1021 gcacttgtcc atggatgcgc caaacgcccg gatctgccgc gcatcgtgcc gatgggcgtt
     1081 acctcgctgg acagaactcc aaggcgcgag gaactcgatt ccctatctgc atccgatccg
     1141 ccagagcgag catgtcggca cgctcatcgg atgacagcgg ctgcgcgtcc tcgctgagca
     1201 cgtcggccaa ctgttccaag gccagaccca gttcgttggc gtcgatgaac tcggcgatca
     1261 gcgcgacgtc cttgctggga aggcggtcgc ccagccggat cagcaggccg tgcaggaggc
     1321 cggcgatctc ctcgtagtag gcgcgatcca ctttcggatc gtgccacagc agtacacgct
     1381 catgccgcga tgtgactcac gtcgaatctt ctttgacgtc gctgcaactc gatgcgacca
     1441 cgtccacgta cggccctccg ctccaccgtg gggtcacgtg agcggcccac acgccagcga
     1501 atggatagcc ttctactgtg ccaacagatt cactaggtcg agaggagcgg catggctgat
     1561 gtgacgggac aggtgcagtt gagcccagat gcgctaagcc gactgctgga tcaagacgct
     1621 atgcgggcga tcctgagtcc tgatgccaag gtccctgggg cctccaatcc cgaggtccaa
     1681 ctggcggagt tctgggaagt cattgacatc gtccgatcgt gcgctggcaa ggtgttcacc
     1741 tcgccagcgg aagccgaagc cgagcttttc gggaccgcga gcggtggcgg tttcggcatt
     1801 cgacaagatc ttcgtgacgc ggtctctggc cttctggtgt ccatcgacgc cgcacccaac
     1861 caagggggag ttgcgccatc gggagggcat gcaacctccg gtggcaaatg gctggctgcg
     1921 gccactgttg cgcaggtgtc ggagcgactg ggcaactacg tgtacgtact cgtggacccc
     1981 cgggactccc ggccgttcta cgtgggtaag ggcattggtc tacggatgat gtcccacggc
     2041 ctggaagccc tcgatgcaca tggggacacg gacggcaaga aggtggcacg gatcaaggag
     2101 atccgcgccg ccggccttga gcacgagata tggatcgttc ggtacggcct gacgcgctcg
     2161 gagtacacag ccgtcgaggc cgccgtgatc gacactttgg ggtcgttccc cgttgtgccg
     2221 tccgctaagg cgtaccgccc gctggagcta cgcgaggaac tcaccaatat gagacgcgag
     2281 gacgcaaagg gaaaggggat gatccttctc gagcgtctag tcgacgaact tgcggcccct
     2341 cctctgacaa ccgctgctcc cctgctgctg atcaccctga gcgcttggac cgatgaacag
     2401 atggacgttg ccggtggccg gacaaggcac ggcaatggct tcaagcggga gtggtacgag
     2461 cctgccacac gggacatgca catccacgag ctcgagatgt cgaccacggc ctggtggaaa
     2521 ctcagccgaa agtcagtcga ggccagaggc atccaccacg ccgtggcagt gcaccgggga
     2581 gtgacccgag gcttgttcgc gatcaaaacg gacagctggg cgtccgccaa cggtcgaagc
     2641 ggcttcgtgg gtgagcccgt cttggctggc gatctctacg acgaggtcgt gggtcctcac
     2701 ggccaccgag tcaccacaaa gcaacgcggc acccagaacc cgatcgccta ctggccacgc
     2761 gcataacaac gtccgcccac cgcctgcaag cagtcgctgc tctcgacctg gctaggacag
     2821 agcaatcgcg ctccaaccga tggatgcagc tgcagtcgtc tcaggcgctt cgccgttccc
     2881 cgcgctgctc gcggcgtagt gacgcaccca ggactgtccg cgcgtgtgac ccagcgtgcg
     2941 ccgagttccg tgcacggccg gcaccgagcg agtggcgccg gctgggggcc taggagcgtg
     3001 ggtcagtaac acgccagtcg ggttccgctg ctgaagtggg c
//
LOCUS       DAOMZY010000392         3041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_31635_length_3041_cov_5.069324, whole genome shotgun sequence.
ACCESSION   DAOMZY010000392 DAOMZY010000000
VERSION     DAOMZY010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31635_length_3041_cov_5.069324"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PLK64_15905"
     CDS             complement(<1..474)
                     /locus_tag="PLK64_15905"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HPV81279.1"
                     /translation="MTDNATPRGEDWAGVVDDLNRLLRLKTTPVGLSRFATLAELEAV
                     PKVRRPDGIHTFDQVVAMAARLGWTVGATADDFVQKQCSAVLGLIPRDEEWLAGRALA
                     GVWFATIEDSAAHQRSMDTAPYAGHAGVVVSPLATGRLDPPDVVLIYATPGQMVLM"
     gene            complement(512..1210)
                     /locus_tag="PLK64_15910"
     CDS             complement(512..1210)
                     /locus_tag="PLK64_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81280.1"
                     /translation="MLTWPEEPEVTDWEPVDDATLAGRLGALIPTSGAALVLIDGRSG
                     NGKSTLADRVCRLFGAGLIHTDDVAWHLHATDWADALVGGVLEPWRRGDAVSFRPPGW
                     VARDRPGSIELAPTRILVVEGVGAGRAELARQADVVIWVQSDQAEARRRGVVRDVALG
                     RPPTEAEAFWDEWASSEDPFLAADRPWTRADFIVNGTPPRADDRTWLAPGPIGTATGP
                     LDPPNEPIGTSPVA"
     gene            1342..1686
                     /locus_tag="PLK64_15915"
     CDS             1342..1686
                     /locus_tag="PLK64_15915"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HPV81281.1"
                     /translation="MAQYLFSVWHIGAYPTPSEEEMAAMFAAVGAFNDKMRDAGALVF
                     GCGIEDPASSVIVDGTGAEPTTRPGRFAAGPEILGGFWVIEAPSVEAAHELAAEASAA
                     CGVPLEIRPLQG"
     gene            1812..2402
                     /locus_tag="PLK64_15920"
     CDS             1812..2402
                     /locus_tag="PLK64_15920"
                     /inference="COORDINATES: protein motif:HMM:NF022995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HPV81282.1"
                     /translation="MTHIHGYTMGHVGAATAAAAVSGEGLSQLRASVLFGDDDRAALR
                     RAGELLDDQIDALLDVWYGFVGSQPHLLAQFSNPSGAPIGDYLDKVRARFGQWVRDTC
                     AGDYGDDWLAYQHEIGRRHARVAKNTTDGADSTAYVPMRDLIALIVPITVTVRPFLAG
                     RANADDDVDAMMGAWLKAVVLSVALWTQPYVADGQW"
     gene            2477..2974
                     /locus_tag="PLK64_15925"
     CDS             2477..2974
                     /locus_tag="PLK64_15925"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPV81283.1"
                     /translation="MSTAQGLSPIQVRILGLVAAAAVRPSELAGRLGVTRATVTDAVR
                     ALEGKGLVAVATDPLDGRSRLLRVTEDGQAVAGRVASWGMPVTHHLAALSSGDQGALL
                     SGLLHLLTGLQADGLVPRARMCLTCRFFREASDAGGVAHCELLRADLPDTSLRLDCPD
                     HEASG"
BASE COUNT          457 a         1088 c         1063 g          433 t
ORIGIN      
        1 catgagcacc atctggcctg gcgtggcata gatgagcacg acgtcgggtg gatctagccg
       61 ccccgtcgcg aggggagaca cgacgacacc ggcgtggccg gcatacgggg ccgtgtccat
      121 cgagcgctgg tgcgccgcgg agtcctcgat cgtcgcgaac cagaccccgg caagcgcgcg
      181 tccggccagc cactcctcgt cgcgcgggat caggccaagc accgccgaac actgcttctg
      241 gacgaagtcg tcggccgtcg cgcccacggt ccacccgagt cgcgccgcca tcgcgaccac
      301 ctggtcaaac gtgtgtatgc cgtccggccg gcgaaccttc ggcaccgcct ccaactcggc
      361 gagggtcgcg aaccgggaga gccccactgg ggtggtcttg agccggagca accggttgag
      421 gtcgtcgacg accccggccc agtcctcgcc tcgcggcgtc gcgttgtcgg tcatgctccg
      481 acgctaatgc gcgggcaacc atcgtggtcc gttaagcgac gggcgaggtg ccgatcggct
      541 cgttcggtgg gtcgagcggc ccggtcgccg tgccgatcgg gccgggggcg agccaggtcc
      601 ggtcgtcggc gcgtggggga gtcccgttaa cgatgaagtc ggcccgggtc cacggccgat
      661 cggcggcgag gaaggggtcc tccgaactgg cccactcgtc ccagaaggcc tcagcctccg
      721 tcgggggccg accgagcgca acgtcccgca cgacacctcg tcggcgcgcc tcggcctgat
      781 ccgattggac ccagatgacg acatcggcct gacgcgccaa ctccgcccga ccggcgccga
      841 ccccctcgac gacgaggatc cgggtgggcg ccagctcgat ggaaccgggc cgatcacggg
      901 ccacccaccc gggcggccgg aacgacacgg cgtccccgcg gcgccagggc tcgagcaccc
      961 caccgacgag agcgtcggcc cagtccgtgg catggaggtg ccaggccacg tcgtcggtgt
     1021 ggatgagtcc ggctccgaac aggcggcaca cccggtcggc cagggtcgac ttcccgttgc
     1081 ccgaccgtcc gtcgatgagc accagggcag cacccgaggt cgggatcagc gcgccgaggc
     1141 gccctgcgag ggtcgcatcg tcgacgggct cccagtcggt cacctcaggt tcttcgggcc
     1201 aagtcagcac ccgatggagt atgccgcctt gccgcggggt cgtccgtcgc cacgcctggc
     1261 cacactgggg aggctgctcg accggtgtcg gcgggacggc ataccgtgag ggcgcactca
     1321 acccgaggaa agaggcgacc tatggcgcag tacctgttct ccgtgtggca catcggtgcc
     1381 tacccgactc cgtccgagga ggagatggcc gcaatgttcg cggccgtcgg cgccttcaac
     1441 gacaagatgc gtgacgcggg cgcgctggtc ttcggttgcg ggatcgaaga cccggcctcg
     1501 tcggtcatcg tcgacggcac gggagccgag cccacgacgc gaccgggtcg gttcgctgcc
     1561 ggccccgaga tcctcggcgg cttctgggtg atcgaggcac cgagcgtcga ggcggcgcac
     1621 gagttggccg ccgaggcctc cgcagcctgc ggtgtcccac tggagatccg ccccctgcag
     1681 ggctgaccgc aagcagccga accgccccga cggaccacaa cagaccgcgc agctcgccgc
     1741 gaggtctgga caatcccttc agcatcagca atagtgagga gtccttagca acatcgatca
     1801 caggaggaac gatgacgcac atccacgggt acaccatggg ccacgtcggg gccgcaaccg
     1861 cagcagcggc ggtgtcgggc gaggggctca gccaactgcg cgccagcgtc ctgttcggcg
     1921 acgacgaccg cgccgccctg cgccgggccg gcgagttgct cgacgaccag atcgacgccc
     1981 tcctggacgt ttggtacggc ttcgtcggca gccagccgca cctgctggcc cagttcagca
     2041 acccgtccgg cgcccccatc ggcgactacc tcgacaaggt ccgtgctcgt ttcgggcagt
     2101 gggtccgcga cacctgcgcg ggcgactacg gcgatgactg gctggcctac cagcacgaga
     2161 tcggccgtcg ccacgcccgg gtcgccaaga acaccaccga cggagccgac tccacggcat
     2221 acgtgccgat gcgtgacctc atcgcgctca tcgtgccgat caccgtgacg gtccgcccgt
     2281 tcttggcggg tcgcgctaac gccgatgacg acgtggacgc catgatgggc gcgtggctca
     2341 aggcggtcgt gctcagcgtc gcgctgtgga cccagcccta cgtcgcggac gggcagtggt
     2401 agcaggcgtc ccctccgcga cgatcaccga cgccctggag cggctcggcc aggtgcagcg
     2461 tgtcctcctt caagaggtgt cgactgcaca ggggctgagc ccgatccagg tgcgcatcct
     2521 cgggctcgtg gcggccgccg cggtgcggcc gtccgagctg gcagggcgcc tcggggtcac
     2581 ccgagcgacc gtcaccgatg ccgtgcgggc tctggagggc aagggcctcg tggccgtcgc
     2641 gacagacccc ctcgacggcc gcagccgcct gctgcgcgtc accgaggacg gccaggccgt
     2701 cgcgggtcgt gtcgcgtctt ggggtatgcc ggtcactcac catctcgcgg cgctttcctc
     2761 gggcgaccag ggggcactgc tctcgggcct cctgcacctg ttgaccggcc tgcaggcgga
     2821 cgggctcgtg ccgcgcgccc ggatgtgcct cacgtgtcgg ttcttccggg aagcatccga
     2881 cgctggtggg gtcgcgcact gcgagcttct ccgggccgac ctgcccgaca cctccctgcg
     2941 cttggactgc ccggaccacg aagcgtccgg ctgacttcgg cgagcggggc gaacagggcg
     3001 ccccgacgag agtgcggtgc acggcatacg gacgcggtga c
//
LOCUS       DAOMZY010000393         3033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_31737_length_3033_cov_5.494291, whole genome shotgun sequence.
ACCESSION   DAOMZY010000393 DAOMZY010000000
VERSION     DAOMZY010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31737_length_3033_cov_5.494291"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PLK64_15930"
     CDS             complement(<1..361)
                     /locus_tag="PLK64_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HPV81284.1"
                     /translation="MVKSLEAIGVDTVFGLPGGAILPAYDPLMDSTKVRHILVRHEQG
                     AGHAAEGYAVASGRIGVCMATSGPGATNLVTPIADAYMDSVPMVAITGQVNSAAIGTD
                     AFQEADIRGITMPITKHN"
     gene            complement(749..1555)
                     /locus_tag="PLK64_15935"
     CDS             complement(749..1555)
                     /locus_tag="PLK64_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(G),N(G)-dimethylarginine
                     dimethylaminohydrolase"
                     /protein_id="HPV81285.1"
                     /translation="MLRRLALVRQPSPRLADGIVTHIDRESVDPVLARDQWEAYVGAL
                     RANGWDIVQAPAAPDCPDGVFIEDQVLVYGDLALLCRSGAPERRAEQVGLAGLLTDVG
                     YRVARIEAPGLLDGGDVLKHDSRVWVGDAGPTGRSNRAGVEQVADLLAPLGVRVEAVP
                     LSGVLHLKSAVTALPDGRILAGPSLSAVTAEWPGWGTSSRGASRLLAVPEESGAHVVI
                     LDADTVLLAASAPQTAEVLTGQGLRVVTVDISEFEKLEGCVTCLSVRLRR"
     gene            complement(1669..>3033)
                     /gene="ilvD"
                     /locus_tag="PLK64_15940"
     CDS             complement(1669..>3033)
                     /gene="ilvD"
                     /locus_tag="PLK64_15940"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPV81286.1"
                     /translation="TRLDLIDAMSKAVDDHVSDADIATIERSACPTCGSCSGMFTANS
                     MNCLLEVVGLALPGNGSTLATAAPRKGLFESAGRLVVELAKRWYAVDGTEDASVLPRS
                     IVTREALSNAMRVDIAMGGSTNTILHLLAAAQEADIAFDQHDIDALSRVTPCLCKVAP
                     NTNRYYMEDVHRAGGIVAIMGELNRAGMLDDSVHAVHAPSLQAWLDEWDIRGGKTSDA
                     AYELFHAAPGGVRTTEAFSSTNRWATLDTDAREGCIRSVEHAYTVDGGLAVLTGNLAP
                     DGCIVKTAGVPEHQWTFRGPAKVAESQDAAVEMILAGNVVAGDVVVVRYEGPKGGPGM
                     QEMLYPTSYLKGVGLGPKCALITDGRFSGGSSGLSVGHVSPEAASGGAIGLVRDGDEI
                     EFSIPERRVTLHVSDDELDRRRLEQDAAGWQPVAREREVSAALRLFALFAQSADKGAA
                     RKVP"
BASE COUNT          468 a         1034 c         1075 g          456 t
ORIGIN      
        1 agttgtgctt ggtgatcggc atcgtgatgc cgcggatgtc ggcctcctgg aaggcatccg
       61 tgccgatggc ggcgctgttc acctggccgg tgatggcgac catcgggacc gagtccatat
      121 aggcgtcggc gatcggggtg accaggttgg tcgctcccgg gccggaggtc gccatgcaca
      181 cgccgatccg gccggacgcc acggcatacc cctcggctgc gtgacccgcg ccctgctcgt
      241 gccgcacgag gatgtgacgg accttggtgg agtccatcag ggggtcgtag gcggggagga
      301 tcgcgccgcc gggcagcccg aagacggtgt cgacgccgat ggcctcgagc gacttgacca
      361 gcgactgggc acccgtgcag ggctccggaa cggccggaac cgcaggggcc gccggggcgc
      421 tcgggcgctg ccggttctgg gcagcgaggt cggccgggga cgccttcgtg gacgtcaggc
      481 tgtcggtcac ggtgttcacc acttcttcgt tgcgtcggac gtgcgaactg atgccggtgg
      541 ggttctcgtc ccacgaaaaa gcccttcagt ccaccggcag gacggaaggg cgcgcgcgtg
      601 actacagagg cgtcacacgc gcctgttgag tacgaggacc tgggtcacaa gaccacggtc
      661 ctttgcgggg gtatgccgtg tcaaccgcgg acgggcctcg tctcaccatc cgagccacgg
      721 agttcacgcc gggcacctgc gagttgggtc agcggcgcag gcgcaccgac aggcaggtga
      781 cgcagccctc aagcttctcg aactcgctga tgtcgacggt gacgacccgc aagccctggc
      841 cggtcaacac ctcggcggtc tgcggcgccg aggccgccag cagcacggtg tcggcgtcga
      901 ggatgacgac atgagctccg gactcctccg gaactgccag gaggcgggag gcacccctcg
      961 agctggtgcc ccagccgggc cattctgcgg tgacggcgct caggctgggg ccggcaagga
     1021 tgcggccgtc gggcagcgca gtcacggccg atttcaggtg caggaccccg gacaacggca
     1081 ccgcttcgac gcggacaccc agcggggcca gcaggtcggc cacctgctcg acaccggccc
     1141 ggttggacct gcccgtcggt cccgcgtcgc cgacccagac ccggctgtcg tgcttgagga
     1201 cgtcacctcc gtcgagcagt ccaggcgcct cgatgcgggc cacccggtag cccacgtcgg
     1261 tcaggagacc ggccaggccg acttgttcgg cccggcgttc tggagcgccg ctgcggcaca
     1321 ggagcgccag gtcgccgtag acgaggacct ggtcctcgat gaacaccccg tcgggacagt
     1381 ccggggccgc cggcgcctgg actatgtccc agccgttggc ccgcaaggca ccgacgtagg
     1441 cctcccactg gtcgcgggcg aggaccgggt cgacgctctc gcggtcgata tgagtgacga
     1501 tgccgtcggc caggcgcggg gacggctgac ggacgagagc aaggcgacgg agcatgcggg
     1561 cattctgccg tggagctcgc gcggcgcccg gtatctcggc gggtgctcag cgagaccgca
     1621 acaactccag cacctcgtca agtttgccct cgaccaggcg ggcagacctc acgggacctt
     1681 gcgggcggct cccttgtcgg cggactgggc aaagagcgcg aacaggcgca gcgctgccga
     1741 gacctcccgc tcccgggcca ccggctgcca ccctgcggcg tcctgctcca gacgtcggcg
     1801 gtcgagttcg tcgtccgaca cgtgcagcgt gacccggcgc tcggggatgg agaactcgat
     1861 ctcatcgccg tcacggacca gcccgatggc tccaccggag gccgcctccg gtgacacgtg
     1921 acccaccgac aaccccgacg agccgcccga gaaccgcccg tcggtgatca gcgcgcactt
     1981 cggccccagg ccgacgccct tgaggtagga cgtcgggtag agcatctcct gcatcccggg
     2041 cccacccttg gggccttcgt agcggacgac gacgacgtcc ccggcgacca cgttcccggc
     2101 gaggatcatc tcgacggcag catcctggct ctcggccacc ttggccggcc cacggaaggt
     2161 ccactggtgt tcgggcacac cggccgtctt gacgatgcag ccgtccgggg cgaggtttcc
     2221 agtcagcacg gccaaaccgc catcgacggt gtaggcgtgc tccaccgagc gaatgcagcc
     2281 ttcgcgtgcg tcggtgtcga gggtcgccca gcggttggtc gaggagaacg cctcggtcgt
     2341 gcgcactccg ccgggggccg cgtggaagag ctcgtatgcc gcgtcgctcg tcttcccccc
     2401 gcggatgtcc cactcatcga gccaggcctg cagcgacgga gcatggacgg catgcaccga
     2461 gtcgtcaagc atcccggccc ggttcaactc gcccatgatc gcgacgatgc caccggcccg
     2521 gtggacgtcc tccatgtagt agcggttggt gttcggggcg accttgcaca ggcacggagt
     2581 caccctggag agggcgtcga tgtcgtgttg gtcgaaggcg atgtcggcct cctgagcagc
     2641 cgcgagcagg tgcaggatcg tgttggtcga tccgcccatg gcaatgtcca cgcgcatcgc
     2701 gttggacagc gcttcgcggg tgacgatcga gcgcggcagg acgctcgcgt cctcggtgcc
     2761 gtccacggca taccagcgct tggccagctc gacgacgagg cgaccggcgc tctcgaagag
     2821 gcccttgcga ggggcggccg tggccagggt cgagccgttg cccggcaggg ccaggccgac
     2881 gacctcgagg aggcagttca tcgagttcgc cgtgaacatg cccgagcagg agccacaggt
     2941 cgggcaggcg gagcgctcga tcgtcgcgat gtcggcatcg gagacgtggt cgtcgaccgc
     3001 cttgctcatc gcatcgatga ggtccagcct cgt
//
LOCUS       DAOMZY010000394         3031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_31769_length_3031_cov_5.484543, whole genome shotgun sequence.
ACCESSION   DAOMZY010000394 DAOMZY010000000
VERSION     DAOMZY010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31769_length_3031_cov_5.484543"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PLK64_15945"
     CDS             <1..782
                     /locus_tag="PLK64_15945"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPV81287.1"
                     /translation="TYWGFRLTRHTGGTLHISRGLITARATSLEERRIRGVEIGQPLG
                     LRLAGAARLSAATSGLGQEGARSGSDWLTPPAPAEVVDRTAVEVVGDEVAVRGPLLSH
                     GPAARRRRFTRAILPAAVLCAATLLLTRATDDLPTALVVAGCLPLLASPWLAADRYAA
                     LGHALTPRHLIVRSGTFSRRRVVLARDGVIGVTIRESFFQRRAGLATVTATTAVGRQH
                     YDAIDVPVELSTGLAADLLPGPFAELLDAEHVPQPSSSDRA"
     gene            complement(895..1656)
                     /locus_tag="PLK64_15950"
     CDS             complement(895..1656)
                     /locus_tag="PLK64_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPV81288.1"
                     /translation="MALSTQAGDFVLKGGVLMAAFADRRPTRDIDLLASGFANEIAEC
                     EGRVRDIANIPFDDGLTFDRSSVKGEFIRDEADYTGVRVHISATLASAQLGIHVDISF
                     GDPIWPGPTETDLPRLLGGTVQLLGYPDHMVLAEKLVTAIQRGTANTRWRDFVDVYAI
                     AEGRPIAQRDLRKSLATVALHRGANLRPLAQALAGMAEAAQPQWAAWRRRQRLDGTTP
                     EKFQDLLDAVIRFADPALGDEPTSRAWNPQRREWL"
     gene            complement(1785..2339)
                     /locus_tag="PLK64_15955"
     CDS             complement(1785..2339)
                     /locus_tag="PLK64_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81289.1"
                     /translation="MLTLSSAAQAGLRKDQIYALTESGELERIGRGVFVDPTRIDPAW
                     TSLAAATALKPAATLCLTSALVHHGLSDAIAFTTDIALPRGSRHPAGFHHVSWHSFDP
                     DTFHAGRTVTDQSGLMLAVYSAERTIIDCFRLAHHQGADQAYEALRRWVRQPGNQPSE
                     LLALAAASFPRNLPRVRAALEVLL"
     gene            2509..>3031
                     /locus_tag="PLK64_15960"
     CDS             2509..>3031
                     /locus_tag="PLK64_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPV81290.1"
                     /translation="MSKVLSAVAWPYANGPRHIGHVAGFGVPSDVFSRYMRMAGHDVL
                     MVSGTDEHGTPILVQADKEGVTPRALADRFNRVIAEDLVGLGLAYDLFTRTTTPNHYS
                     VSQELFRGNHANGYMIEQTQLVAISPSTGRTLPDRYIEGTCPICGYGEARGDQCDNCG
                     NQMDPAELIDPRSK"
BASE COUNT          453 a         1001 c         1064 g          513 t
ORIGIN      
        1 tgacctactg ggggttccgg ctcacgcggc ataccggcgg caccttgcac atcagccgcg
       61 gcctcatcac cgcgcgggcg acgagcctgg aggagcgacg gatccgcggc gtcgagatcg
      121 gccagccgct gggtctgcgc ctcgcgggtg cggcccggct cagcgcagcg acgagcggac
      181 tgggccagga gggtgcccgc agcggcagcg attggctcac ccctccggcg cccgccgagg
      241 tcgtcgaccg caccgccgtc gaggtggtcg gagacgaggt ggcggttcgc ggtccactcc
      301 tgtcacacgg ccccgcggct cggcgtcggc ggttcacccg cgccatcctc cctgcggccg
      361 tcctctgcgc cgcaaccctg ttgctgaccc gtgccaccga cgacctgccg acggcgctcg
      421 tcgtcgcggg ctgcctcccg ctgctcgcct cgccctggct cgccgccgac cggtatgccg
      481 cgctcggcca cgcgctgacc ccgcggcatc tgatcgtccg atcggggacg ttcagccggc
      541 gccgcgtggt cctcgcccgg gacggcgtca tcggggtgac gatccgggag tcgttcttcc
      601 aacgccgcgc tgggctggcc accgtgacgg cgacgacggc tgtggggcgg cagcactacg
      661 acgcgatcga cgtgccggtg gagttgtcga ccggcttggc cgccgacctg ctgcccggtc
      721 cgttcgccga gttgctggac gcggaacacg tccctcagcc aagctcgagt gaccgcgcct
      781 agcttcctac cgagggccga acggccgtca gcccacctga gcaggggcat cagccgcgcg
      841 ggggcttctt cgcttcccgc acttcaacgc ccgggggctg aatccccagg acggtcacaa
      901 ccattccctt ctctgcggat tccatgcccg gctcgtgggt tcgtcgccaa gtgccgggtc
      961 ggcgaaccgg atcactgcgt cgagtaggtc ctggaacttc tccggtgtgg tgccgtcgag
     1021 gcgttgcctg cgtcgccagg ccgcccactg gggctgagcg gcttcggcca tgccggcgag
     1081 ggcttgggca agggggcgga gatttgcccc tcggtgcagg gcgacggtcg ccagtgattt
     1141 gcgcagatct cgttgcgcga tgggccggcc ctcagcgatc gcgtacacgt cgacgaagtc
     1201 gcgccagcgg gtgttggcgg ttccgcgctg gatggcggtg acgagcttct cggcgaggac
     1261 catgtggtcg gggtacccca ggagctggac cgttccaccg aggaggcggg gaagatcggt
     1321 ttctgtcggc ccgggccaga tcggatcgcc gaagctgatg tcgacgtgga ttcccagttg
     1381 cgcgctggcc agggttgcgc tgatgtggac gcggacaccg gtgtagtcgg cttcgtctcg
     1441 gatgaactcg cccttgactg aggaccggtc gaatgtcagg ccatcgtcga aggggatgtt
     1501 ggcgatgtcg cggacgcggc cctcgcactc ggcgatctcg ttggcgaagc cggatgccaa
     1561 cagatcgatg tcgcgggtgg gccgcctgtc ggcgaaagct gccatcagca cgcctccctt
     1621 gagaacgaag tcacccgctt gcgtcgagag ggccaaccgg gcgagaaacc cctcgagggt
     1681 gtacatcgtg aggtactccg cgacatcccg gccctgcctt cgcgcaagat tgcgaagatc
     1741 gttgtacgcc cgtcctgccg gtgtgtctcg ggttggcctg ggcgtcacag gaggacttcc
     1801 agggccgcgc ggacgcgggg caggttcctc gggaatgagg cggcggccag tgccaacagc
     1861 tccgatggtt ggttccccgg ctgacggacc cagcggcgca gcgcctcgta ggcctggtct
     1921 gccccctggt gatgggccaa gcggaaacag tcgatgatcg tgcgttcagc ggagtagacg
     1981 gcaagcatga gaccgctttg gtcggtcacg gtcctgccgg cgtggaaggt gtcagggtcg
     2041 aacgaatgcc agctcacgtg gtgaaacccg gcgggatggc ggctgccgcg cgggagcgcg
     2101 atgtcggtag tgaatgcgat ggcatcgctc aggccgtggt gcactagggc gcttgtcagg
     2161 cacagggtgg ccgccggctt gagtgccgtc gccgcggcca gcgatgtcca ggcaggatca
     2221 atccgggtgg ggtcgacgaa gaccccgcgc ccgatccttt cgagttcgcc ggactccgtg
     2281 agcgcgtaga tctggtcctt ccgaagtccc gcctgcgctg cggaggagag ggtgagcact
     2341 ggactgccca tggtcgctcc taactgtcta caggtgaatc atatctgtaa acagttagag
     2401 acggtgtgaa ggttggagct catcgcgacg gagcgcccga ctccgcgcca ctggctgttg
     2461 gccgtgagcc cggaaacgtg gttgctgcag acctctagga tcgagcccat gagcaaggtc
     2521 ctgtccgccg tcgcctggcc gtatgccaac ggcccgcgcc atatcggcca tgtcgccggt
     2581 tttggtgtgc cctccgacgt gttcagccgc tacatgcgga tggccggcca tgacgtgctc
     2641 atggtgtcgg gcaccgacga gcacgggacg ccgatcctcg tccaggccga caaggagggc
     2701 gtcaccccgc gcgcgttggc cgaccgcttc aaccgggtca tcgccgagga cctcgtcggt
     2761 ttgggcctgg cctatgacct gttcacccgg acgacgacgc ccaaccacta ctccgtgagc
     2821 caggagttgt tccggggcaa ccacgccaac ggctacatga tcgagcagac ccaactcgtg
     2881 gcgatcagcc cgtcgacggg gcggacgctt ccggaccgct atatcgaggg cacctgtccg
     2941 atctgtggtt acggcgaggc ccgcggcgac cagtgcgaca actgcggcaa ccagatggac
     3001 ccggccgagc tcatcgaccc gcgcagcaag a
//
LOCUS       DAOMZY010000395         3019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_31964_length_3019_cov_5.918016, whole genome shotgun sequence.
ACCESSION   DAOMZY010000395 DAOMZY010000000
VERSION     DAOMZY010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31964_length_3019_cov_5.918016"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..2069
                     /locus_tag="PLK64_15965"
     CDS             90..2069
                     /locus_tag="PLK64_15965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPV81291.1"
                     /translation="MSAPAVLWGQGWVDADDSTLLALVRDGNAEAYAELWRRHLPAAY
                     GVAHRYRGRTSAEDVVAEASLRVYDLIRAGKGPLTNFRSYFLSAVRTIAVDHARGDLR
                     AVPTEDASLEAAAPATAAYDPSPTVDHALVRAAFARLAERDQQVLWQTAVEGTTPAAV
                     ADSMGLTANTVSVIALRARDSLRAKYLDAHADRAISRAQDAECAWVLGQMGRFVRGKL
                     PVRQRARVEEHLRSCRHAQGLAYEMAEVNRALPAVIVPLIFLAGSSLVAVWAGAAGAA
                     GGVGASGASVGPREEGKAAVAAALAANVGTKIAALGVAAVLATGYAAAAGVGGGGGGA
                     AGGSGNGGAGPVAAAPVVPGAVPAQPRTAAPSQEAAPSQTAVPSPTAVPSATSSTTTT
                     ARSSSTTTTSASTTTSSTSTTPTPTPTPSPSTTPSPSTTPTPTTSSPTSTTPPVPSIP
                     LVTPVAAAFGEPFPVEIVVPLVGVSSVVLKSVPVGQLSLTVTPAVAAADATSWVCDDQ
                     GSQITCTGSGSTTFSVLATDSRQGLGAARMASTSDASKPATVTVELTDPDGTVTLAEV
                     ILGSVTEPSPTTQPTATSTPTGTTDPTGTTEPSVTTDPSVTTDPSVTTDPNVNNSPTA
                     TSDPAATSTPTLTTVVVTVSPSGAVVVTDTTRPVG"
     gene            complement(2088..2693)
                     /locus_tag="PLK64_15970"
     CDS             complement(2088..2693)
                     /locus_tag="PLK64_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPV81292.1"
                     /translation="MTGKERREQLVDVGRAVFAAKGFEAATVEEIAAKAGVSKPVVYE
                     HFGGKEGLYAVVVDREMVALLQAVTSALETPGPTRAVVESAALALLDYVEGSTDGFRI
                     LVRDSPAGQSTGSFASLIGDIATQVEAILAAEFSRRKLDPKVAPIYAQMLVGMVALTG
                     QWWLEHRRFKKADVAAHLVNLAWNGLIALDNKPTLHTAKTR"
     gene            2842..2920
                     /locus_tag="PLK64_15975"
     tRNA            2842..2920
                     /locus_tag="PLK64_15975"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2877..2879,aa:Gln,seq:ttg)
BASE COUNT          429 a         1081 c         1023 g          486 t
ORIGIN      
        1 agaacttccg ccgaaacggg tgtcatgggc acaatggttc tgcgtctgtc atatgcaggg
       61 gtctagcttg aggaagggag gggtctgaca tgtccgcgcc agccgtactg tgggggcagg
      121 gatgggttga cgctgatgac agcaccctcc tggccctggt tcgtgatggc aacgccgagg
      181 cgtatgccga gttgtggcgc cgccacctcc cggcggccta tggcgtcgcc caccgctacc
      241 gcgggcggac ctcggccgag gacgtcgtcg ccgaggccag ccttcgggtc tatgacctca
      301 tccgggccgg taagggcccg ctgaccaact tccggtcgta cttcttgtcg gcagtgcgca
      361 ccatcgccgt cgaccatgcc cgaggtgacc tgcgtgccgt cccgaccgag gacgcttctc
      421 tggaggcagc ggctccggcc acggcggcat acgacccgag ccccaccgtc gaccacgccc
      481 tggtccgcgc ggccttcgct cgactcgccg agcgtgatca gcaggtcctc tggcagaccg
      541 cagtcgaggg caccacgcct gccgccgtcg ccgactccat ggggctgacc gccaacaccg
      601 tcagcgtcat cgcgctgcgg gctcgcgaca gccttcgtgc gaagtacctt gacgcacatg
      661 ccgatcgggc gatcagccgg gcccaagacg ccgaatgcgc atgggttctg ggccagatgg
      721 gtcgcttcgt ccggggcaag ctcccggtcc gtcagcgcgc ccgcgtcgaa gaacacctgc
      781 gctcgtgtcg gcacgcccag ggcctggcct acgagatggc cgaggtcaac cgcgccctgc
      841 ctgcggtcat cgttccgctg atcttcctgg ctgggtcgtc actcgtcgcc gtctgggccg
      901 gtgctgccgg tgctgccggt ggcgtgggtg cgtccggcgc ctctgtgggg ccccgcgagg
      961 agggcaaggc ggcggtcgcg gccgcgctcg ccgccaacgt cgggaccaag atcgctgcgt
     1021 tgggtgtggc cgccgtgctt gccacggggt atgccgcggc ggcgggcgtc ggtggcggcg
     1081 gaggtggcgc tgctggaggg tccggcaatg ggggagccgg tcccgtcgct gctgcgccag
     1141 tggtgcccgg tgcggtgcct gcgcagccgc gcaccgccgc gccctcgcaa gaagcggcgc
     1201 cgagccagac cgcggtgccg tcgccgacgg cggttccttc tgcgacatcg agcacgacca
     1261 cgaccgcgcg gtcatcgagc accacgacga catcggcctc gacaacgacc tcctcgacgt
     1321 caacgacgcc gacgccgacg ccgacgccgt ccccgtcaac gacgccgtcc ccgtcaacga
     1381 cgccgacgcc gaccacttcg tctccgacga gcaccacacc tccggtgccg tcgatacccc
     1441 tggtgacgcc ggttgctgcg gcgttcgggg agcccttccc ggtcgagatc gtcgttcccc
     1501 tcgtcggggt ctcgtcggtg gtcctcaagt cggtccccgt cggccagctc tcgctcacgg
     1561 tgaccccggc ggtcgccgct gccgacgcca cgagttgggt ctgcgacgac cagggctcgc
     1621 agatcacctg cacgggctct gggtcgacga cgttctcggt cttggcgacg gactcccgcc
     1681 agggtctcgg cgctgcacgg atggctagca cgtccgacgc gtcgaagcct gccactgtga
     1741 cggttgagct gaccgatccc gacgggacgg tcacgctggc cgaagtcatc ctcggttcag
     1801 tgaccgaacc gtcgcccacg acccaaccga cggccacgtc gactcccacg ggcaccaccg
     1861 acccgacggg gacgacggag ccctcggtca cgacggaccc ctcggtcacg acggaccctt
     1921 cggtcacgac ggaccccaat gtcaacaaca gcccgactgc gaccagtgat cctgcggcca
     1981 cgtcgacccc gactctgacg accgttgtcg tgacggtcag cccgtcgggg gccgtcgtgg
     2041 tgaccgatac gacccgcccg gtcggctgac gtcgagtccc gcgaaggtca gcgggtcttg
     2101 gcggtgtgca gggtcggctt gttgtccagc gcgatcagcc cgttccaggc caggttgacc
     2161 aggtgtgccg cgacgtcggc cttcttgaac cggcggtgct ccagccacca ctgacccgtg
     2221 agggcaacca tccccacgag catctgcgcg tagatcggtg ccaccttggg gtcgagcttc
     2281 cggcgggaga actccgcggc caggatggcc tccacctggg tggcgatgtc accgatgaga
     2341 ctggcgaacg acccggtcga ctgcccggca ggggagtcgc gcacgaggat gcggaacccg
     2401 tcggtcgacc cttcgacgta gtccagcagc gccaacgccg ccgactccac gacggcgcgg
     2461 gtcgggcccg gcgtctccag cgcggaggtg accgcctgca gaagtgccac catctcgcgg
     2521 tcgacgacga cggcatacag gccctctttg ccgccgaagt gctcatagac gacgggctta
     2581 ctcacccccg ctttcgccgc gatctcctcg actgtggcgg cctcgaaacc cttggcggcg
     2641 aacactgccc gccccacgtc gacgagttgc tcccgccgct ccttgcccgt catccgggcg
     2701 cggcggctgg ggggcgcact ctcacgtggg gtcacggtgc cattgtgccc ggtggtatgc
     2761 cgtgcgcccg ccacccgtgt gacgggtaac ctaggcgggt ccttcgtcga tccggcacgc
     2821 gccgggccgc gagggtgccg atccccggtt ggtctgctgg cagggcccgc ctgactttga
     2881 atcaggatta gcgacgctgg ttcgattcca gcccggggag caacaccgct gtcacgcgct
     2941 ggcgaccggc tactcgcaca ggtgcgggtt gtaggtgatc tggacgaccg tggtgttggc
     3001 cttgtccagg gaccaccag
//
LOCUS       DAOMZY010000396         3013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_32059_length_3013_cov_6.101420, whole genome shotgun sequence.
ACCESSION   DAOMZY010000396 DAOMZY010000000
VERSION     DAOMZY010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32059_length_3013_cov_6.101420"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="PLK64_15980"
     CDS             <1..722
                     /locus_tag="PLK64_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPV81293.1"
                     /translation="NINTAGQVVAAGTLEQLRALAAAPPAKARVIPLQVAGAFHTQHM
                     APAVDRLAAYARAMTTREAHTKLISNRDGHVVRSGREVLNRLVTQVSSPVRWDLCMQT
                     MLDLGVTGLIEIPPAGTLVGLAKRAMPGVELLAIKGPEDLDAARRMVADHGDRPDPSA
                     DPEWSLVIAPTKGTLSFGSVAVGDQISRGIVLAQVRTADGDVAVTAPHSGWLSQWLVR
                     DGDDVGPGQPVLRLAPKGALS"
     gene            719..1744
                     /locus_tag="PLK64_15985"
     CDS             719..1744
                     /locus_tag="PLK64_15985"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPV81294.1"
                     /translation="MTVEALRPKGSGSIHPGTGASHARIWGLGGFRPERVVPNSELVE
                     AIDSSDEWIQERSGIKSRRWAGPNDTVVSMSEAAARDAIAAAGIEISQIEAIIVSTVT
                     HPYQTPAAAPIIADRLGLRCAAFDISAACAGYCHGISLANDMVRGGSAKFVLVIGVEK
                     LTDFTDLTDRGTAFIFADGAGAAVVGPSDFPGIGPTIWGSDGSQWETIRQTESWLDVR
                     DQGLGHPTIGMAGQSVFRWAVWSMAPVARQAIEAAGITPDDLDAFIPHQANMRIIDAM
                     LKQLKLPADIPVARDIAETANTSAASIPLATERMLREGEIPHGGLALQIGFGAGLVYA
                     AQVIVLP"
     gene            1820..2068
                     /locus_tag="PLK64_15990"
     CDS             1820..2068
                     /locus_tag="PLK64_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPV81295.1"
                     /translation="MAQSTQEILEGLAEIVNEETGIDTGSVELDKSFTDDLDIDSLSM
                     MTIVVNAEEKFNVRIPDDEVKNLKTVRDAVDFIAAAQG"
     gene            2219..>3013
                     /locus_tag="PLK64_15995"
     CDS             2219..>3013
                     /locus_tag="PLK64_15995"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HPV81296.1"
                     /translation="MSSSDIRVVVTGLGATTPVGGTVPETWEAILAGRSGARTMDFDW
                     VAKYELPVTFAAQIHTPPDQVLAKVETRRLDPSSQYALIAAREAWADAGAPELDPERL
                     AVAVGSGIGGVWTLLSQWDTLREKGPRRVYPLSVPMLMPNGPSAAISLELGARAGAHT
                     PVSACASGAEGMGYAVQMIRSGRADVVVAGGTEAAIHPMPIAGFAAMQALSTRNDDPA
                     AASRPYDKDRDGFVLGEGAAIIVLESEAHARARGAKIYAELAGVGMS"
BASE COUNT          479 a         1105 c          987 g          442 t
ORIGIN      
        1 ccaacatcaa caccgccggg caggtcgtcg ccgcagggac gctcgagcag ttgcgggccc
       61 tcgccgccgc gccaccggcc aaggcccgcg tcatcccctt gcaggtggcg ggcgcgttcc
      121 acacccaaca catggccccc gccgtggatc ggctcgcggc ctacgcccgc gcgatgacca
      181 cccgcgaggc ccacaccaaa ctcatctcca accgcgacgg gcacgtcgtg cgttccgggc
      241 gcgaggtgct caaccgcctc gtgacccagg tcagctcgcc ggtgcgctgg gacctgtgca
      301 tgcagacgat gctcgacctc ggcgtcaccg gcctcatcga gatcccgcct gccggcaccc
      361 tcgtcggtct ggcgaagcgg gccatgcccg gggtcgagtt gctggcgatc aaggggcccg
      421 aagacctcga cgcggcccgg cgcatggtcg ccgaccatgg cgatcgcccg gacccgtcag
      481 ccgatcccga gtggagcctg gtcatcgccc cgaccaaggg cacgctgagc ttcggttcgg
      541 tcgccgtcgg cgaccagatc agccggggca tcgtcctggc gcaggtgcgc accgcagacg
      601 gcgacgtcgc agtgaccgcg ccgcacagcg gctggctgag ccaatggctc gtccgcgatg
      661 gagatgacgt cggcccgggc caaccggtcc tgcggttggc cccgaaggga gcactgtcgt
      721 gactgtcgag gcacttcggc ccaagggctc cggctcgata cacccgggaa cgggtgcgag
      781 ccacgcccgc atctggggcc tcggcgggtt ccggcccgag cgggtcgtgc ccaactccga
      841 gctcgtggag gccatcgact cctccgacga gtggatccag gagcggtccg gcatcaagtc
      901 acggcgttgg gctggtccga acgacaccgt ggtgtcgatg tccgaggcgg ccgcgcgtga
      961 tgccattgcg gctgcaggca tcgagatctc ccagatcgag gcgatcatcg tctcgacggt
     1021 gacccacccc tatcagacgc cggcggcagc gccgatcatc gccgaccggc tcgggctgcg
     1081 ctgcgccgcc ttcgacatct cggccgcctg cgccggctat tgccacggca tctccttggc
     1141 caacgacatg gtccgcggcg gcagcgcgaa gttcgtcctc gtcatcggcg tcgagaagct
     1201 caccgacttc accgacctca ccgaccgtgg cacggcgttt atcttcgccg acggcgccgg
     1261 tgcggccgtg gtcggcccct ctgacttccc gggtatcggc ccgacgatct ggggatcgga
     1321 cggctcgcag tgggagacca tccgccagac cgagtcgtgg ctggatgtgc gcgaccaggg
     1381 cctggggcac cccaccatcg gcatggccgg tcagtcggtg ttccgctggg ccgtgtggag
     1441 catggcaccg gtggcccggc aggcgatcga ggccgccggc atcaccccgg acgacctgga
     1501 cgcctttatc ccccaccagg ccaacatgcg gatcatcgac gcgatgctca agcagctcaa
     1561 gctcccggcc gacatccccg tcgcccgcga catcgccgaa accgccaaca cctcggcggc
     1621 atcgatccct ctggccaccg agcggatgct gcgcgaaggt gagatcccgc acggcgggct
     1681 cgctctgcag atcggcttcg gggccggcct ggtgtatgcc gcgcaggtca tcgtcctgcc
     1741 ctgacagatt cccgcgcctc gcgggtcaac gaggcaccca gatcctcgaa aagttttgtc
     1801 cacgaaggag atacacacca tggcacagag cacccaggag atcctcgaag gtctcgccga
     1861 gatcgtcaac gaagagaccg ggatcgacac cggctcggtc gagttggaca agtcgttcac
     1921 cgacgacttg gacatcgact cgctgtcgat gatgacgatc gtcgtcaacg ccgaggagaa
     1981 gttcaacgtg cgcatcccgg acgacgaggt caagaacctc aagaccgtgc gcgacgctgt
     2041 cgacttcatc gcggccgccc agggctgagc cccacccggt cgagcgctgc cacgcgatcg
     2101 accagcgcca ccgcttgtgg cggggccccg ctggaaccgc agccggggca cccgccgcaa
     2161 gcggacggca taccccatga atcacgtgct gtccccacca accaggagcc gaaaccccat
     2221 gtcctccagc gacattcgcg tcgtcgtcac cggcctcggg gccaccacgc ccgtgggcgg
     2281 caccgtccca gagacctggg aggccatcct cgccggtcgc tcgggcgccc ggaccatgga
     2341 cttcgactgg gtggccaaat acgaactgcc ggtgactttc gccgcccaga tccacactcc
     2401 cccggaccag gtgctggcca aagtggagac ccgacggctc gacccgtcgt cgcagtacgc
     2461 cctcatcgcc gcccgcgagg cctgggccga cgcaggtgcc cccgagctcg accctgagcg
     2521 gctggccgtc gcggtcggct ccggcatcgg cggcgtgtgg actctgctgt cgcagtggga
     2581 cacgctgcgc gagaagggcc cccgccgcgt ctacccgctg tcggtcccca tgctgatgcc
     2641 caacggacct tccgcggcga tctcgctcga gctcggcgcg cgagccggtg cgcacacccc
     2701 cgtcagcgcc tgtgcctccg gtgccgaggg catggggtat gccgtgcaga tgatccgcag
     2761 cggccgcgcc gacgttgtcg tcgctggcgg gaccgaagcg gcgatccacc cgatgccgat
     2821 cgctggtttc gctgcgatgc aggccctttc gactcgcaac gacgaccccg ccgccgcttc
     2881 acgcccttac gacaaggacc gtgacggctt cgtgctcggc gagggcgcgg cgatcatcgt
     2941 cctggagtcc gaagcacacg cgcgggcccg cggcgccaag atctatgccg agttggcggg
     3001 ggtcgggatg agc
//
LOCUS       DAOMZY010000397         2994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_32384_length_2994_cov_3.894862, whole genome shotgun sequence.
ACCESSION   DAOMZY010000397 DAOMZY010000000
VERSION     DAOMZY010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32384_length_2994_cov_3.894862"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PLK64_16000"
     CDS             complement(<1..245)
                     /locus_tag="PLK64_16000"
                     /inference="COORDINATES: protein motif:HMM:NF020897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV81297.1"
                     /translation="MNYLESVDNSLRLLMLVAAKARVGVSEVAAELGVAPSTAYRLLS
                     TLRYRGFVVQGHDRTYRAGPALEEMVSNRITRANLADL"
     gene            344..1936
                     /locus_tag="PLK64_16005"
     CDS             344..1936
                     /locus_tag="PLK64_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81298.1"
                     /translation="MALELGVNLPVGADGSRSSTASAQTVLAASVAGVDDTLAADIMR
                     ARDWRHRYPEYFTRILVAEAQSGSAALQIARQGLAAARSHYVVVAADGTQEPLPSAVT
                     RRGLDLRTVAVSGHDERVRELVVPHRGENLRGLALLRQLDDWVRRGIVEPTFADAVGA
                     VVRNPDWLDLRGHTFALVGAGAQMGPFAQLVQWGARVAAIDLPRPDVWKRLLSVVRES
                     AGTMYVPVHADHEDPVEADIPTRAGADLMTDVGPLVDWLAGLSGPMTVGNYGYADGVL
                     FVRLSMAFDALLEGLRSRRDDLSVTYLATPSDVFLVPMNAVDMAQERHSRQSPLIVAG
                     KVAHAMSAGRWFGPNYGRGGVIETPTERFGVVSAFIVAQGPNYAMAKRLQRWQMIATR
                     ADGILTSVHVAPPTRTSSVHSNPIMAERQRLTAYVGIETFDAPTTEAIAGAILVHDLH
                     NPASPANPANPLGHPHEAFMFAANPGGRWRVPFDVGSTVPLLHEITGARLRAGSLIRD
                     LSAHVPGLDRLPGLGRLGTSTD"
     gene            complement(2026..>2994)
                     /locus_tag="PLK64_16010"
     CDS             complement(2026..>2994)
                     /locus_tag="PLK64_16010"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPV81299.1"
                     /translation="HDGIRQMFEIITYVRMMVGLKTVSALSSGHLNARDYAMSRKQGR
                     ELISLTGARGGKPLVEIVDHPDIRRSLLTQKAYAEGGRALVLYTATMIDRFEVAKALG
                     GSDTVAVERHRLLLPIVKTWCAESGWRVLGQEALQVFGGSGYLRDYPLEQYVRDTKID
                     AIYEGTTGIQSLDLFERRLVRDRGATAGVLLDEIAQTVDDLATGALATESVLLGDALA
                     AFRGILEHTFAQADAAPRVAALGLTRLLMSMGDLIVGWLLLRTAAAALARQSDPSLLK
                     TADADLAGSVAAGRWFARQVLPHLTAELVAARLLDEEPLLLPDASL"
BASE COUNT          464 a         1067 c         1017 g          446 t
ORIGIN      
        1 agatcggcga ggttggcccg ggtgatccgg ttgctcacca tctcctcgag cgccggcccg
       61 gcccggtagg tgcggtcgtg cccctgaacg acgaaccccc gataccgcag ggtggacagg
      121 agtcggtatg ccgtcgacgg cgccaccccg agctcagccg caacttcgct cacacccacc
      181 cgagccttgg cggcgacgag catgagtagg cgcaaggagt tgtcgacaga ctccaagtag
      241 ttcatccgcg cacgaccccc gttcctccag tcgaagatgt ggcccaggac acgttacggc
      301 caggggttgc gaggtggaaa ccctccggag aaagcagatc cgcatggcac ttgaactcgg
      361 cgtcaatctc cccgtcggcg ccgacggctc gcgcagcagc accgccagcg cccagaccgt
      421 gcttgcggcc tcggtcgccg gcgtcgatga cacactcgcc gctgacatta tgcgtgcccg
      481 cgactggcgc caccgctatc ccgaatattt cacccggatc ctcgtcgccg aagcgcagtc
      541 cgggtcggcc gccctgcaga tcgcccgcca ggggttggcc gccgcacgga gccactacgt
      601 cgtcgtcgcg gcggacggca cccaggagcc gctgccctcc gcagtgacgc gccggggcct
      661 ggacctgcgg accgtcgccg tcagcggtca cgacgagcgg gtgcgcgagc tggtcgttcc
      721 ccaccgcggc gagaaccttc ggggtctggc gctgttgcgc caacttgacg actgggtgcg
      781 ccgcggcatc gttgagccga ccttcgcaga cgcggtcggg gcggtcgtgc gcaaccccga
      841 ctggctggac ctgcgcggtc acaccttcgc cctcgtcggc gccggcgccc agatgggccc
      901 gttcgcccaa ctcgtccagt ggggcgcccg ggtggccgcc atcgatctgc cgcggcccga
      961 cgtctggaag cgactgctga gcgtcgtgcg cgagtcggcc ggcacgatgt atgtcccggt
     1021 ccacgccgac cacgaggatc cggtcgaagc cgacatcccc acccgcgcgg gcgccgacct
     1081 catgaccgat gtcggacccc tggtcgactg gctcgccggg ctgagcggcc cgatgacggt
     1141 cggcaactac gggtatgccg atggcgtcct cttcgtgcgc ctgtcgatgg ccttcgacgc
     1201 gttgctcgaa ggcttgcggt cgcgccggga cgacctgtcc gtcacctacc tggccacccc
     1261 ctcggacgtc ttcctcgtcc cgatgaacgc cgtcgacatg gcacaggagc gacactcgcg
     1321 gcaaagcccg ctcatcgtcg ccggcaaggt ggcccatgcg atgagcgcgg ggcgctggtt
     1381 cgggcccaac tacggtcggg gcggggtcat cgagaccccg accgaacgat tcggggtcgt
     1441 cagtgccttc atcgtcgccc aagggcccaa ttacgccatg gccaagcgtc tgcagcggtg
     1501 gcagatgatc gcgacccggg cggacggcat actcacctcg gtgcatgtgg caccccccac
     1561 caggaccagc tccgtgcact ccaacccgat catggccgag cggcaacggc tcaccgccta
     1621 cgtggggatc gagacgttcg acgccccgac caccgaagcc atcgccggcg ccatcctggt
     1681 ccatgacttg cacaacccgg catcgccggc caacccggcc aacccgctgg gacacccgca
     1741 cgaggcgttc atgttcgcgg cgaaccctgg ggggcggtgg cgagtgccct tcgacgtcgg
     1801 cagcaccgtt ccgctgctgc acgagatcac cggtgcccga ctgcgcgccg gatcgctgat
     1861 ccgcgacctg tcggcccacg ttcccgggct ggaccgcctg cctggcctgg ggcgcttggg
     1921 cacgtcgacg gactgacccg ccggcaggcg cgggcggcat acctcttgcg cctgcaggcg
     1981 ggaagcctcc cgtggggtgg ggcggcgtcc ctggcgtcgg tgtgctcaca gggacgcgtc
     2041 ggggaggagc agcggctcct cgtccagcag acgcgcagcg accaactcgg ccgtgaggtg
     2101 cgggagcacc tgccgcgcga accagcgccc tgctgccacc gacccggcca ggtcagcatc
     2161 agcggtcttg agcaggctcg gatcgctctg gcgagccagt gccgcggcgg cggtgcgcag
     2221 caacagccag ccgacgatga ggtcgcccat cgacatgagc aaccgggtga ggcccagggc
     2281 ggcgacccgg ggcgccgcat cggcctgcgc gaaggtgtgt tcgaggattc cgcggaaggc
     2341 agccagggcg tctcccaaca ggaccgattc ggtcgcgaga gcgccggtgg ccaggtcgtc
     2401 gacggtctgg gcgatctcgt cgagcagcac cccggcagtg gcgccccgat cgcgcacgag
     2461 gcgccgctcg aagaggtcga gggactggat gccggtcgtg ccttcgtaga tcgcgtcgat
     2521 cttggtgtcg cggacgtact gctccagcgg gtagtcgcgc aggtagcccg atccaccgaa
     2581 aacctggaga gcctcctggc cgaggacccg ccaaccgctc tcggcgcacc acgtcttgac
     2641 gatgggcagc agcaggcggt ggcgttcgac cgccacggtg tcgctgccgc ccagcgcctt
     2701 ggcgacctcg aaccggtcga tcatcgtcgc ggtatagagg acgagagcac ggccaccctc
     2761 ggcataggcc ttctgggtga gcagcgaccg acggatgtcg gggtggtcga cgatctccac
     2821 cagcggcttc ccgccgcgag ccccggtcaa cgagatcaac tcgcgcccct gcttgcgact
     2881 catcgcgtaa tcgcgggcat tgaggtgccc gctggagagg gccgagaccg tcttgaggcc
     2941 gaccatcatc cggacgtagg tgatgatctc gaacatctgc cggatgccgt cgtg
//
LOCUS       DAOMZY010000398         2972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_32745_length_2972_cov_5.709976, whole genome shotgun sequence.
ACCESSION   DAOMZY010000398 DAOMZY010000000
VERSION     DAOMZY010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32745_length_2972_cov_5.709976"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PLK64_16015"
     CDS             complement(<1..415)
                     /locus_tag="PLK64_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81300.1"
                     /translation="MTAVLRWGLAGLACAVVARVGMMLLTRHPEPTRSGTVAILVVFT
                     LGGLAVGVVDAARRAGRTPWTRLVALVGVGIFLGAGAVFLPSTLVGGAVLSRRRVPTW
                     ARLAAVVAVLGWGMAMVRIASADNDPLGLYVATPRF"
     gene            complement(412..951)
                     /locus_tag="PLK64_16020"
     CDS             complement(412..951)
                     /locus_tag="PLK64_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HPV81301.1"
                     /translation="MGLSNSRRVPRTVTAAAILVVLGIPVAWGAAALVTGASLSDLQP
                     AAASAFDGASAKVTVVSHRDSTTTVLRVHGVGAAAVGKTYGAHLHEGMCVAGDGGAAG
                     GHYNHTKILGDPITISAQTEVWLDVTIDETGDATAVSHVPFAVVPGQRSVVIHAVATD
                     PDTGLAGPRLACLPVEWTP"
     gene            1138..1650
                     /locus_tag="PLK64_16025"
     CDS             1138..1650
                     /locus_tag="PLK64_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPV81302.1"
                     /translation="MPDPAGGIDPAGLIATLFQAEAARLTSLARFFVDDRTAAEDLVQ
                     EAFIRLSRSLDRIRDEERATAYLRSIVINLARDHNRRGLMSFRHRPPAQPDEPSAEAT
                     AADLESRAEVIAELRRLPLRQRDCLVLRYYLDLGIDEIAATLQISRNSVKTHLQRGLR
                     TMESSLEARQ"
     gene            1863..2270
                     /locus_tag="PLK64_16030"
     CDS             1863..2270
                     /locus_tag="PLK64_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81303.1"
                     /translation="MNWWILEVATNVALIAIALWLGPFIKRFGRAYAADVFYDNPLTG
                     KSYIVLTDVVYYLIFTAYILFTVRFAPTVDWANTFSERVTAEQLAFEVGRIGGILLII
                     GILHGLNIVLMPVLGRLFSLNRRLPRGGAHPEG"
     gene            complement(2343..>2972)
                     /locus_tag="PLK64_16035"
     CDS             complement(2343..>2972)
                     /locus_tag="PLK64_16035"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HPV81304.1"
                     /translation="EEHDFHDFKISVKHNDPVIMVKAYEMLAERGDWPLHLGVTEAGP
                     AFQGTIKSAVAFGALLSRGIGDTIRVSLSAPPVEEVKVGNQILQSLGLKPRKLEIVSC
                     PSCGRAQVDVYTLADQVTAGLEGLTVPLRVAVMGCVVNGPGEAREADLGVASGNGKGQ
                     IFVRGEVIKTVPESQIVETLIEEAMRLAEEMGEPVDGDAGAGAPTVTVS"
BASE COUNT          505 a         1077 c          928 g          462 t
ORIGIN      
        1 ggaagcgagg cgttgccaca tacagcccca gcgggtcgtt gtccgcgctg gcgatgcgaa
       61 ccatcgccat cccccagccg aggactgcga caaccgcagc cagccgcgcc caggtcggga
      121 ctcgtcgacg cgagaggacc gcgccgccca cgagcgtcga cggcaagaac accgcgcctg
      181 caccgagaaa tatgcccacc ccgacaaggg caacgagacg ggtccacggc gtgcgtccgg
      241 cccggcgagc ggcgtccacg accccgaccg ccaagccgcc aagcgtgaag acgaccagga
      301 tggccacggt gcccgaccta gtcggttccg gatgcctggt cagcagcatc atgccgacgc
      361 gggccacgac ggcgcaggcc aacccggcga ggccccatcg gagaaccgct gtcatggtgt
      421 ccactccacc ggcagacagg ccaggcgcgg gccggccagc ccagtgtcgg gatcggtcgc
      481 gacggcgtgg atgacgaccg accgctgacc cggcaccacc gcgaacggga cgtgcgacac
      541 cgccgtcgcg tcgccggtct cgtcgatcgt gacatcgagc cagacctcgg tctgggcgct
      601 gatcgtgatc gggtcgccga ggatcttcgt gtggttgtag tgcccgccgg cagccccgcc
      661 atcgccggcc acgcacatac cttcgtgcag atgggcgccg taggtcttcc cgaccgctgc
      721 cgcaccgacg ccgtgcacac gcaataccgt cgtcgtcgag tctcggtgtg agaccacggt
      781 gaccttggcc gacgccccgt cgaaggccga tgccgccgcc ggctgcagat ccgagaggct
      841 cgcacccgtc accaaggcgg ccgcgcccca ggcaaccggt atccccaaaa ccacgaggat
      901 cgcagcagcc gtgaccgttc tgggaacgcg gcgagaattc gacagaccca tgatgttctc
      961 ctcgtgctgg ggtggttcag ccggtgcggc cggatacctg aatcgacgga caccggcggt
     1021 ctccgggcga caccatgact atgacgcgaa tctcacacga ccggtttgtc gcccgggaag
     1081 ccgtcccact cgtctgtcga tgcaatggag gccactgccg tcccacgagt gacgccgatg
     1141 ccagacccgg ccggcgggat cgatcctgcc gggctcatcg ccacgctctt ccaggcggag
     1201 gccgcccggc tcacctccct cgcccggttc ttcgttgacg accgaacggc cgcggaggac
     1261 ctggtccagg aggccttcat caggctctcg cgctcgctcg accggatccg cgacgaggag
     1321 cgggccacgg catacctgcg ctcgatcgtc atcaacctgg cgcgggacca caaccggcgg
     1381 ggcctgatgt ctttccgaca ccgcccaccc gcccagccgg acgaaccgtc ggccgaagcc
     1441 accgccgctg acctggagag tcgtgccgag gtcatcgccg agctgcgacg gctgcccctg
     1501 cggcagcgtg actgcctcgt ccttcgctac tacctcgacc tgggaatcga cgagatcgcc
     1561 gccactttgc agatctcgcg caactcggtg aagacccacc tgcaacgtgg cctgcgcacc
     1621 atggaatcca gtttggaggc tcgacagtga ccacgaccga gtcgcgccta cgcgacgccc
     1681 ttgccgaccg gaccggccgg cagccatcgc ctgacctctt cgaacgcgtt ggccggtcga
     1741 tcgccgatga cctgacccgc cgtgcccgcc gccgccgtgc cgggtgggtc ggtgccggcc
     1801 tgacggtcgt ctgtggcgcg gccctcatcc tgttggttcc gcgagactcg aaaggactga
     1861 tcgtgaactg gtggatcctc gaggtcgcga caaacgtggc cctgattgcc atcgccctct
     1921 ggctcggccc cttcatcaag aggttcggcc gggcgtatgc cgctgacgtc ttctacgaca
     1981 atccgctgac cggcaagagc tacatcgtgc tcacggacgt tgtctactac ctcatcttca
     2041 cggcatacat cctgttcacg gtgagattcg caccgacggt cgattgggcc aacacgttca
     2101 gtgagcgcgt caccgccgag cagttggcgt tcgaggtggg gcggatcggg gggatcctgc
     2161 tcatcatcgg catcctgcat ggactgaaca tcgtcctcat gcccgtcctc ggacggctct
     2221 tctccctcaa ccgacgactg ccgaggggcg gcgctcaccc ggagggatga ccctccccat
     2281 cagcccggtc gaggtattcc gccgtcacca gacgatggcg gaatacctcg aatcagcaat
     2341 tgtcagctga cagtcaccgt gggcgcgcca gccccagcgt cgccgtcgac cggctcgccc
     2401 atctcctcgg ccaagcgcat ggcctcctcg atgagggtct cgacgatctg gctctcgggc
     2461 acggtcttga tgacctcgcc cctgacgaag atctgaccct tgccatttcc cgacgcgaca
     2521 ccaagatcgg cctcgcgagc ctcgcccggc ccgttgacga cgcagcccat gacggcaacc
     2581 cgcaagggca cggtcagccc ctccagaccg gccgtcacct ggtcggccag cgtgtagacg
     2641 tcgacctgag cgcgcccaca cgaggggcac gagacgatct cgagcttgcg gggcttgagc
     2701 cccaacgact gcaggatctg gttgccgacc ttgacctcct cgaccggagg agccgacagc
     2761 gagacccgga tcgtgtcgcc gatgccgcgt gagagcagcg ccccgaaggc aaccgccgac
     2821 ttgatcgtcc cctggaaggc cggccccgcc tcggtcacgc cgaggtgcag cggccagtcg
     2881 ccccgctcgg cgagcatctc gtaggccttg accatgatga ccgggtcgtt gtgcttgacc
     2941 gagatcttga agtcgtggaa gtcgtgctcc tc
//
LOCUS       DAOMZY010000399         2952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_33041_length_2952_cov_5.921643, whole genome shotgun sequence.
ACCESSION   DAOMZY010000399 DAOMZY010000000
VERSION     DAOMZY010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33041_length_2952_cov_5.921643"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1467
                     /locus_tag="PLK64_16040"
     CDS             <1..1467
                     /locus_tag="PLK64_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HPV81305.1"
                     /translation="DVLDVWVHVGAGRQIAAEGVTWTRAVTLYQGKELFTQGFVDRGR
                     SPFPPFVNIGQNRTEELLDEQIRANPLITEKWGHTVESVEQDADSITVVCRVGTHGAT
                     ETVRITGAYVIAAPGNKGHLVRDMVGMTFEGTTFDDQFLICDIRTTLPGWELERRFYF
                     DPEWNPGRQVLIHPCPDSTYRIDWQVPPNYDLASEEASGALDKRIRQIVGDVDYEIVW
                     KSAYRFHARQVDQMVAGRLLMAGDGAHLVAPFGARGLNSGVPDAENAAWKVAFVMHGW
                     ADPSLISTYHDERHAAAAENLDVTAATMRFLVPQNEQEWASRREILEGAAAHPDDLGL
                     RAQVNSGRMAEPFWYVDSPLTTPSADHPFTGRPPKGEDPVAAPGVIAPDVEVTLAGGD
                     RVRIRALARRGLLVLVGADLDAEAVAEAMAGLRCQTFVLPLAEVDPDGSVAQALGAHP
                     GEAWVFRPDAHIAAVATSATDAASAAYRAVGATAPATV"
     gene            1565..2749
                     /locus_tag="PLK64_16045"
     CDS             1565..2749
                     /locus_tag="PLK64_16045"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HPV81306.1"
                     /translation="MAYYRSAGNIPHKRHVQHRDPEGKLYYEELMGEEGFSSDSSLLY
                     HRFIPAAIREVRPWTLPDQTLRANDPMIPRHFKTQSLPGDGAGIDAVNGRRLLLGNGD
                     VRLSYVVAKDPSPYYRNAIGDECLYVHGGTARVETVFGDLDVWEGDYVIVPRATTHRI
                     VPTGDAPVRIYAIEVNSHITPPKRYLSRFGQLLEHSPYCERDLRVPTDPYVVEETDIP
                     VHIKHRGAGANGISGTIHVQPHHPFDVVGWDGCLWPYAFNISDYEPLTGRVHQPPPTH
                     QVFEAHNVIMCNFVPRKVDYHPLAIPVPYYHSNVDSDEVMFYAAGDYEARRGSGIAAG
                     SISLHPMGHAHGPQPGAYERSIGIESFDETAVMVDTFAPLLMGEGALAVEDPAYPSSW
                     NE"
BASE COUNT          465 a         1110 c          930 g          447 t
ORIGIN      
        1 gacgtgctcg acgtgtgggt ccacgtcggt gccggacgtc agatcgccgc cgagggcgtc
       61 acctggaccc gggccgtcac gctctaccag ggcaaggagc tcttcaccca gggcttcgtc
      121 gaccgcggcc gctcaccctt cccgccgttc gtcaacatcg gccagaaccg caccgaggag
      181 ttgctcgacg agcagatccg cgccaacccc ctcatcaccg agaagtgggg tcacaccgtc
      241 gagtccgtcg agcaggacgc cgacagcatc accgtggtat gccgcgtcgg cacccacggc
      301 gcgaccgaga ccgtgcggat caccggcgcc tacgtcatcg cggcgcccgg caacaagggt
      361 cacctcgtgc gcgacatggt ggggatgacc ttcgagggca cgacgttcga cgaccagttc
      421 ctcatctgtg acatccgcac caccttgccg ggatgggagt tggagcggcg cttctacttc
      481 gaccccgagt ggaacccggg ccgccaggtc ctgatccacc cgtgccccga ctcgacctat
      541 cggatcgact ggcaggtgcc gccgaactac gacctcgcct ccgaggaggc ctccggggcg
      601 ctggacaagc ggatccgtca gatcgtcggc gacgtcgact acgagatcgt ctggaagtcg
      661 gcctaccgct tccacgcccg ccaggtcgac cagatggttg ccggtcgcct cctcatggcc
      721 ggtgacggcg cgcacctcgt agcgccgttc ggtgctcgcg gcctcaactc cggcgtcccc
      781 gatgccgaga acgccgcctg gaaggtcgcc ttcgtcatgc acggctgggc cgacccgtcg
      841 ctcatctcga cctatcacga cgaacggcac gccgcggctg ccgagaacct cgacgtcacc
      901 gccgcgacca tgcgcttcct cgtcccgcag aacgagcagg agtgggcgag ccgtcgcgag
      961 atccttgagg gcgcggcagc gcaccctgac gacctcggcc tgcgggccca ggtcaactcc
     1021 ggccggatgg ccgagccgtt ctggtatgtc gactccccgc tgaccacccc gtcggccgac
     1081 caccccttca ccggccgccc gcccaagggc gaggacccgg tcgccgcgcc gggcgtcatc
     1141 gcccccgacg tcgaggtcac tctggccggt ggcgaccggg tccggatccg ggcgctggcc
     1201 cgccgcggcc tgctggtcct cgtcggcgca gaccttgacg cagaggccgt tgccgaggcg
     1261 atggccgggc tacgctgcca gacattcgtg ttgcctctgg cggaggtcga ccctgatggt
     1321 tccgtcgccc aggcattggg tgcccacccc ggcgaggcct gggtgttccg gcccgacgcc
     1381 cacatcgcgg cggtggcgac cagcgcgacc gacgccgcgt ccgcggcata ccgcgccgtg
     1441 ggtgcgaccg ccccggcgac cgtgtgacct gccgactgcc gactgccgac cggccgacct
     1501 ggcaacccgc cgacctggca ctgaccgacc cgacgacccg caccgaccaa ggagactcca
     1561 ggacatggcc tactaccgca gcgcaggcaa catcccgcac aagcggcacg tgcagcaccg
     1621 ggaccccgag gggaagctct actacgagga gctcatgggt gaggaagggt tctcgtcgga
     1681 ctcctcgctg ctctaccacc gcttcatccc ggcggcgatc cgcgaggtcc gcccgtggac
     1741 gctccctgac cagacgttgc gcgccaacga cccgatgatc ccgcggcact tcaagaccca
     1801 aagcctgccg ggtgacgggg ccggcatcga cgcggtcaac ggccggcgcc tgctgctggg
     1861 caacggtgac gtccgactgt cgtatgtcgt cgccaaggac ccgtcccctt actaccgcaa
     1921 cgccattggc gacgagtgcc tttatgtgca cggcggcacg gcgcgggtcg agacggtctt
     1981 cggcgacctg gatgtctggg aaggcgacta cgtcatcgtt ccccgcgcga cgacccaccg
     2041 aatcgtcccg accggagacg ccccggtgag gatctacgcc atcgaggtga acagccacat
     2101 cacgccgccg aagcgttacc tctcgcggtt cgggcagttg cttgagcact cgccctactg
     2161 cgagcgcgat ctgcgggtgc ccaccgaccc ctacgtcgtc gaggagaccg acatcccggt
     2221 gcacatcaag caccgcggcg caggcgccaa cggcatctcc ggcaccatcc acgtccagcc
     2281 ccatcacccg ttcgacgtcg tcggctggga cggctgcttg tggccctatg ccttcaacat
     2341 ctccgactac gagcccctca ccggccgcgt ccaccagccg ccgccgaccc accaggtctt
     2401 cgaagcgcac aacgtcatca tgtgcaactt cgtcccgcgc aaggtcgact accacccgct
     2461 ggcgatcccg gtcccctact accactccaa tgtcgactcc gacgaggtga tgttctacgc
     2521 cgcgggtgac tacgaggcgc gtcggggctc cggcatcgcc gccgggtcga tctcgttgca
     2581 cccgatgggt cacgcccacg gcccgcagcc gggtgcctac gagcgctcga tcggcatcga
     2641 gtcgttcgac gagacggccg tcatggtcga caccttcgcg ccgttgctca tgggcgaggg
     2701 cgccctcgcc gtcgaggacc ccgcctatcc ctccagctgg aacgagtagc ccttcccgca
     2761 cggccggttc gcgggcggca tacctcctcg tgggtatgcc gcccgccccc cttcccctcc
     2821 ccaccacgga gatagcgctc gcatggtcac ttgtgaggcg attttgggct ctgagacccc
     2881 caaacatgac cgtgcgagcg ctatctccgt gggggggtgc aggggccgca gcccaggggg
     2941 gtgcaggggc gg
//
LOCUS       DAOMZY010000400         2927 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_33458_length_2927_cov_4.527855, whole genome shotgun sequence.
ACCESSION   DAOMZY010000400 DAOMZY010000000
VERSION     DAOMZY010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33458_length_2927_cov_4.527855"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PLK64_16050"
     CDS             <1..309
                     /locus_tag="PLK64_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPV81307.1"
                     /translation="QQQRVAIARALVNSPELMLLDEVTSALDPELVGEVLDLLVSLKA
                     EGMTMVLATHEMAFAKQVADRVCFLHGGAIHEEGPPAALLGAPSRERTRAFLSRFVGL
                     "
     gene            306..968
                     /locus_tag="PLK64_16055"
     CDS             306..968
                     /locus_tag="PLK64_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005504236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HPV81308.1"
                     /translation="MTGPDAGGAGEVGVGPYAGPPLSGDHWDPVLLAEGDRRNVVDRY
                     RYWRHEAIVADLDTRRHPFHVAVENWGHDFNIGSVIRTANAFNAAAFHIVGRRRWNRR
                     GAMVTDRYQHEHHHPDAAALATWAQAQAGAGAGEASGIPLIGIDNLPGSVALETYDLP
                     RECVLVFGQEGPGLTPEMQQVCRVVLHIDQFGSTRSINAGAAAAIAMHAWVRRHVFDQ
                     QP"
     gene            965..2215
                     /locus_tag="PLK64_16060"
     CDS             965..2215
                     /locus_tag="PLK64_16060"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV81309.1"
                     /translation="MTAPPGQPGPAPAGLGAGYWRMFAADSVSSIGTVVSTLAIQFLL
                     IDTLQADEQAIGLVRAAQWAPYLVFGLLAGVIAERVRRKPLLVAADLLGMTIFGAIGL
                     LALAGRLTVPIVAGLVFVFGAVTCVSVAAYQSYVPRLVPERALPDAFARLEQMGQVVG
                     SVGPVAAGSLVRFLSAPIAVLVDALSYAVSAVVIATIRTPEPPIERAERRHLVAELRE
                     GARWVYRHETLRPYAFWLHSWFVGRAMVMTVLVFYANQELHLDALTVGLILAAAGVSG
                     VVAAGVAPRLGRRFGVGRVITTVEWVSPLWPVLVVLAQPGPWGVAVLVVAMLLDGLTA
                     MSASLMMTYRTAVTPDRLRARMNATIRTFNWGGLAIASFVSGWVASMWGSRVAMLAGA
                     AILLVATAYLRRSAFRSAVLPVAV"
     gene            complement(2234..>2927)
                     /locus_tag="PLK64_16065"
     CDS             complement(2234..>2927)
                     /locus_tag="PLK64_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81310.1"
                     /translation="IEPTKGRRCFGRAGGRQGGQGRAGGGEAEVRCTAYLWAWLAGTV
                     PKHPRTVVLLEGASDVAAVRTLAAAREVTLERVRLVDLGGVTNIRRVLRQARADWADA
                     EVLGLCDAAEVRFIERALVEIGLPVRDPSDLPAYGFFVCHADLEEELLRALGPVRTIA
                     VIERLGLGAKLVALQRQLAWQDRPLAEQLHRFCGVASGRKELLGGALAEALDLDAVPE
                     PLALLLDRLAEA"
BASE COUNT          374 a         1052 c         1024 g          477 t
ORIGIN      
        1 cagcagcaac gggtcgccat tgcccgggcc ctagtcaact cacccgagct catgctcctc
       61 gacgaggtga cctcggccct cgacccggag ttggtcggcg aggtgctcga cctgcttgtc
      121 agcctcaagg ccgaggggat gacgatggtg ctggccaccc acgagatggc tttcgccaag
      181 caggtggccg accgggtgtg tttcctgcac ggcggcgcca tccacgagga aggtccgccg
      241 gcggccctgc tcggcgcccc gtcgcgcgag cgcacccgcg cgttcctgtc ccggttcgtc
      301 ggcctgtgac cggccccgac gcgggagggg cgggcgaggt gggcgtcggt ccgtatgccg
      361 gtccgcccct ctccggcgat cactgggacc ccgtgctgct cgccgagggc gaccggcgca
      421 acgtcgtgga tcgctaccgc tattggcggc acgaggcgat cgtcgccgac ctcgacaccc
      481 gccggcatcc gttccacgtg gcggtcgaga attggggcca cgacttcaac atcggctcgg
      541 tgatccgcac ggccaacgcc ttcaacgcgg cggcgttcca tatcgtcgga cggcgacggt
      601 ggaaccggcg aggggcgatg gtcaccgacc gctaccagca cgaacaccac caccccgacg
      661 ccgccgcgct cgcaacgtgg gcacaggcgc aggcgggggc gggggcgggg gaggcgagcg
      721 gcatacctct catcgggatc gacaacctgc ccggctcggt cgcgctggag acctacgacc
      781 tgccgcgcga gtgcgtcctc gtcttcggcc aagaggggcc cgggctgacg cctgagatgc
      841 agcaggtatg ccgtgtggtc ctgcacatcg accagttcgg ctcgacccgc tcgatcaacg
      901 ccggcgccgc ggcggcgatt gccatgcatg cctgggtgcg ccgccacgtc ttcgaccagc
      961 agccatgacg gcgcctcccg gccaaccggg cccggctccc gctgggctcg gggctggcta
     1021 ctggcggatg ttcgccgccg actcggtctc gtcgatcggg acggtcgtct cgaccctggc
     1081 gatccagttc cttctcatcg acaccctcca ggccgacgag caggcgatcg gcctggtccg
     1141 tgccgcgcag tgggctccct acctggtgtt cgggctgctc gccggcgtga tcgccgaacg
     1201 ggtccgccgc aaacccctgc tcgtggccgc cgacctgctg gggatgacga tcttcggggc
     1261 gatcggcctg ctggccctcg ccggccggct caccgtcccc atcgtcgccg ggctggtctt
     1321 cgtgttcggc gcggtcacgt gcgtgtcggt cgcggcatac cagtcctatg tcccgaggct
     1381 cgtgcccgag cgcgccctcc ccgacgcctt cgcccgtctc gaacagatgg ggcaggtggt
     1441 cggctcggtc gggccggtcg ccgcgggctc gctcgtgcgc ttcctctccg cgccgatcgc
     1501 ggtactcgtc gacgcgctct cgtatgccgt gtccgccgtc gtcatcgcga ccatccgcac
     1561 gccggagccg ccgatcgagc gtgctgagcg tcggcacctc gtcgccgagc tgcgcgaagg
     1621 tgcccggtgg gtctatcggc acgagaccct gcggccgtat gccttctggc tgcacagctg
     1681 gttcgtcggg cgcgccatgg tgatgacggt gctggtcttc tacgccaacc aggagttgca
     1741 cctggatgcc ctcaccgtgg gcctcatcct ggccgccgct ggcgtgtcgg gtgtcgtggc
     1801 cgccggggtg gcgccgcggc tggggcggcg gttcggtgtc ggccgggtca tcaccaccgt
     1861 cgaatgggtg tcgccgctgt ggccggtgct cgtcgtgctt gcacagcccg ggccctgggg
     1921 cgtcgccgtt ttggtcgttg cgatgctgct ggatgggctg accgcgatga gtgcctcgct
     1981 gatgatgacc taccgcaccg cagtcacccc cgaccggctc cgggcgcgga tgaatgccac
     2041 gatccgcacc ttcaactggg gcgggttggc catcgcgtcg ttcgtgtcgg ggtgggtggc
     2101 cagcatgtgg gggagtcggg tcgcgatgct ggcgggagcc gcgatcctgc tggtggcgac
     2161 ggcatacctg cggcgctcgg ccttccggtc cgcggtgctg ccggtcgcgg tctgagcccg
     2221 ggtgagtcga ggctcaggcc tccgccaagc ggtcgagcag gagggcgagc ggctcgggca
     2281 ccgcatccag gtcgagggcc tcggcgagcg cacccccgag caactctttg cgtccggacg
     2341 cgaccccgca gaagcggtgc aattgctcag cgagcggacg gtcctgccag gccaactgtc
     2401 gttgcagcgc aacgagtttg gccccgaggc ccagtctctc gatcacggcg atggtcctca
     2461 ccggcccgag ggcccgaagc agttcctcct cgagatcggc gtggcagacg aagaacccgt
     2521 aggcaggaag gtcgcttggg tcgcgcaccg gcagcccgat ctcgacgagg gcccgctcga
     2581 tgaaacgcac ctctgccgcg tcgcagagcc cgagcacctc ggcatctgcc caatcggctc
     2641 gggcctggcg cagcacccga cggatgttgg tgaccccgcc gaggtcgacc agccggactc
     2701 gttccagggt gacctcgcgg gcagcggcga gggtgcggac ggctgcgaca tcactcgccc
     2761 cctcaaggag gacgacggtc cgggggtgct tcggcacggt gccagccaac cacgcccaga
     2821 ggtatgccgt gcaccgcacc tccgcctcgc ccccgcccgc cctgccctgc ccgccctgtc
     2881 gcccgcccgc ccgcccgaaa catcgtcgac ctttcgttgg ctcgatg
//
LOCUS       DAOMZY010000401         2926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_33475_length_2926_cov_5.165796, whole genome shotgun sequence.
ACCESSION   DAOMZY010000401 DAOMZY010000000
VERSION     DAOMZY010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33475_length_2926_cov_5.165796"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1621)
                     /locus_tag="PLK64_16070"
     CDS             complement(<1..1621)
                     /locus_tag="PLK64_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPV81311.1"
                     /translation="MPPTAWPFVGREPVVADVRSRLDGDRHVVITGAAGLGKTRVAQE
                     IIDRYAASGTPVVRVVASPASAPVPLAPLAALVGDAIGIEAMNAARRSVAALARQGSS
                     RPLLSVDDLHLLDEASAIVIHQLLTTGTVRVLATVRSSATCPPAVDRLRQSTGVDHVE
                     LGTMADADIADLVERALGGPLSGHARSVIITSAAGNPMYARELVEGSVAAGAMQRHGG
                     VYGFEGRMVATPLLEEVVLARLAPFEGAERTALELLAVGGRLEQPLVERVAGFEPLER
                     LERLGILVAEEHAGRLVLDVGHPLYRELTRARLGALARMRIYRTLAEVDAALANPSGT
                     STPDEQLRAAVWSIRGGLPVESVLLTAAAHRAAATGNTPLAAELAHEASRRTGTTQDA
                     MLASWCLSLDGDHDQAIAVLRTALRNETDPWTRAAVRLRIAEEHWWFGDLDSGRAVLD
                     EAAAEPGPWDALREAQLSAHAALAGDVTESVRLGSPLMDHAHPWVRFVAAVGYCLGMI
                     YGDGADEGLRAARALTESLAGTDVQPIGDPGQ"
     gene            1785..2618
                     /locus_tag="PLK64_16075"
     CDS             1785..2618
                     /locus_tag="PLK64_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPV81312.1"
                     /translation="MKIERKVFVVTGGGNGIGREVVLGLLTRGARVAIVDRRTDDILE
                     TIRLAGVGAERVSSHIVDVTDEAAVAALPAQVEQVHGQVDGLVNVAGIIQHFVRFADL
                     TEAEMTSVMDVNFWGVVRMCKGFLPLLSARPEACVVNVSSMGAFVPVPGQTLYGASKA
                     AVKLFTEGLYAELRGTPVAVTVVFPGGVATGIAQNSGARIPGAPEADAGGADADAKKM
                     AASLTSPQDAAAAIIGGIERGSYRVVIGKDATMLDRLSRLAPQRATDLVAKKMASLLG
                     H"
     gene            complement(2677..>2926)
                     /locus_tag="PLK64_16080"
     CDS             complement(2677..>2926)
                     /locus_tag="PLK64_16080"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV81313.1"
                     /translation="HGNERFGDIAKAIPEATDGVLSARLKDLCARGLALRDVTPGPPV
                     SVRYTLTAAGRDLGPVLDALTAYGATHPEVLIPKRPRG"
BASE COUNT          431 a          985 c         1085 g          425 t
ORIGIN      
        1 gctggcccgg atccccgatg ggctggacat cggtgccggc gaggctctca gtgagtgccc
       61 gagctgcccg caacccttcg tcggcgccgt cgccgtagat catcccgagg caatagccca
      121 cggcggcaac gaacctcacc cagggatggg cgtggtccat gagcggtgac cccaaccgaa
      181 ccgactctgt cacgtcgccg gcaagggcgg cgtgagcgga cagttgtgct tcgcgcagcg
      241 cgtcccaagg gcccggttct gcggccgcct cgtcgagcac cgcgcgaccg ctgtcgaggt
      301 cgccgaacca ccagtgttcc tccgcgatgc gcaggcgcac cgcggcgcgg gtccacgggt
      361 cggtctcgtt gcgcagagcc gtgcgcagga cggcaattgc ttggtcgtgg tcgccgtcga
      421 gcgacagaca ccacgacgcg agcatggcgt cctgggtggt gccggtgcgt cggcttgcct
      481 catgggcgag ctcggccgct agcggggtgt tacccgtggc ggccgcccga tgggccgccg
      541 ccgtgaggag caccgattcg accggcagcc cgccgcggat gctccagacg gcagcccgca
      601 actgctcgtc cggggtgctc gtgccggagg ggttggccag agcggcgtcg acctcggcga
      661 gggtgcggta gatccgcatc cgggctagcg ctcccaggcg cgcccgggtg agctcgcgat
      721 agaggggatg cccgacgtcc aggacgagtc tcccggcgtg ctcctcggcc acgaggatgc
      781 ccagccgttc gaggcgctcc aacggctcga agccggcgac ccgctcgacg agtggttgtt
      841 ccaggcgacc cccgacggcg agcaactcca acgccgtccg ctccgcaccc tcgaacggtg
      901 ccaggcgggc gagcaccacc tcttccagca gcggtgtggc gaccatgcgg ccctcgaacc
      961 catagacgcc gccatggcgc tgcatggccc cggcggcgac ggaaccctcg acaagctccc
     1021 gggcatacat ggggttaccg gccgccgagg tgatgatgac gctccgcgcg tgcccagata
     1081 gcgggccgcc gagggcgcgc tcaacgaggt cggcgatgtc ggcgtctgcc atggttccca
     1141 gctcgacgtg atcgaccccg gtcgactggc gcagccggtc caccgcaggt gggcaggtgg
     1201 cggagctgcg gacggtggcc aggacgcgca cggtgcccgt cgtgagcagt tggtggatga
     1261 cgatggcgct ggcctcatcg agcaggtgca ggtcgtcgac cgacagcagg ggacgtgacg
     1321 agccttgccg agccagcgcg gcgaccgacc gtcgggcggc gttcatcgct tcgatgccga
     1381 tcgcgtctcc cacgagggct gccaaggggg ccaacggcac cggcgcggac gcggggctgg
     1441 caacgacccg cacgacgggg gtgccggacg cggcatacct gtcgatgatc tcttgggcca
     1501 ctcgggtctt gcccaggcct gctgccccgg tgatgaccac atggcggtcg ccgtcgagcc
     1561 gcgagcgcac gtcggcgacg acgggctcgc gtccgacgaa cggccaggca gtgggaggca
     1621 cggcagcact ttagggcgtg agagccggcc cgtccgtgtt ctgggttacc ggcgcggcca
     1681 gaggtgagcg acgagacacg ccggacgacc gccggtgtgc gacaacagag ttagcgttga
     1741 ggtgaacccc ggaagagact cggctgggga cgaagggatc gatcatgaag atcgagcgca
     1801 aggtgtttgt ggtcaccggg ggcggcaatg ggatcggccg ggaggtggtc ctcgggctcc
     1861 tcacccgagg cgctcgcgtg gctatcgtcg accggcgaac cgacgacatt ctggaaacca
     1921 tccggctggc gggggtgggc gccgagcggg tgagcagcca catcgtcgac gtcaccgacg
     1981 aggccgccgt ggcggccctt ccggcgcagg tcgagcaggt gcacggccag gtcgacgggc
     2041 tggtcaacgt ggccgggatc atccagcatt tcgtgaggtt cgccgatctc accgaggccg
     2101 agatgacctc ggtcatggac gtcaacttct ggggcgtcgt gcggatgtgc aaggggttcc
     2161 tgccgttgct gtccgcccgc ccggaggcct gtgtggtcaa cgtgtcgagc atgggcgcgt
     2221 tcgtgccggt gccggggcag acgctttacg gcgcgagcaa ggctgcggtc aagctcttca
     2281 ccgaaggcct gtatgccgag ttgcgcggca caccggtcgc cgtcaccgtc gtctttcccg
     2341 gcggcgttgc caccggcatt gcccagaact cgggggcgcg gatcccgggc gccccggagg
     2401 ccgatgcggg cggggccgac gccgacgcga agaagatggc cgccagcctc acctcgccgc
     2461 aggacgccgc tgctgcgatc atcggcggca tcgagcgcgg cagctatcgc gtggtgatcg
     2521 gcaaggacgc gacgatgctc gaccggctct cgcggctcgc cccgcagcgg gccaccgacc
     2581 tggtcgccaa gaagatggcc tccctcctcg ggcactaacc ccggggcggt gcggcggcat
     2641 accctcgcct gacgggtgct ccgcgctcgc gagacttcag ccgcggggtc gcttggggat
     2701 cagcacctcg gggtgggtgg ctccgtatgc cgtgagcgca tctagcaccg gcccgaggtc
     2761 acggccggcc gcggtgaggg tgtagcggac gctgaccggc gggcccgggg tcacgtcgcg
     2821 tagggccagg ccgcgggcgc acaggtcctt gaggcgcgcc gaaagcactc cgtcggtcgc
     2881 ctcagggatc gctttcgcga tgtcgccgaa ccgctcgttc ccgtgc
//
LOCUS       DAOMZY010000402         2909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_33772_length_2909_cov_5.786615, whole genome shotgun sequence.
ACCESSION   DAOMZY010000402 DAOMZY010000000
VERSION     DAOMZY010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33772_length_2909_cov_5.786615"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PLK64_16085"
     CDS             complement(<1..327)
                     /locus_tag="PLK64_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HPV81314.1"
                     /translation="MVGTSEGEARALAALSAAGVAHEVTRHGPVGSLAEAAAARGVRP
                     ADIVKTLVVRRGDGDFLFVLVPGDRKFSWPKLRALLGTSRLSMPDADGAYAATGYVRG
                     IITPFGS"
     gene            532..1857
                     /locus_tag="PLK64_16090"
     CDS             532..1857
                     /locus_tag="PLK64_16090"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HPV81315.1"
                     /translation="MSSFWAEHVWLPSGLARGVRLVVDDGRFVVVEPRATRHPEDVKL
                     RGVVFPGLANAHSHVFQRVLRGRTQSDADNGITWRAKMYDVAERLNPDLYLALARATF
                     AEMALAGITVVGEYHYLHHGPGGSRYSDPNAMGAALARAAHEVGIRLTLLDTLYLQGG
                     LTSEGHLPLDGVQQRFSDGTVEAWAARTARIEQTELLRRGAAIHSIRAVSREDAAYAA
                     QLVGDRPLHMYVSEQPAENLACQMYYGTTPTELLADIGVLGSEFTAVHATHLTERDIS
                     LLADHECQVVVCPTSEQDMADGVPAVRRLLDAGVPVGIGTDQHAFIDPFVEMRELEMH
                     ERLGSGERGRISTSQLLRCGSVHGYQSLGWYDGGSIAPGLVADFITVDLESTRTAGSK
                     AAEVIYTASAADVLTVVVGGRVIVENGQHAFGPVAPLMREALSLIRDAS"
     gene            complement(1891..2184)
                     /locus_tag="PLK64_16095"
     CDS             complement(1891..2184)
                     /locus_tag="PLK64_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4235 domain-containing protein"
                     /protein_id="HPV81316.1"
                     /translation="MGPMVWKVLGTGSAVLAATVAQKLVTKGWELASGKPAPDDPNHP
                     EDTTWKEAVLFAALTGLAVSAARVMATRKAAQYYAKSAGHLPAALVKADGATG"
     regulatory      2272..2374
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2424..>2909
                     /locus_tag="PLK64_16100"
     CDS             2424..>2909
                     /locus_tag="PLK64_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81317.1"
                     /translation="MPFAHPRVRVPLRRSPVRRDPLRKNRFAIPGAVLAVALTASLSA
                     CSLSSTTTATAPATTSGTATASQVSSPSSTAAPRCGAVTEVLLVSHDSFGLSDELTAA
                     FTKATGCTIKVLASGDAGELTNKLALTKASPIGDAVFGIDNTFASPFFFRLELTLPPN
                     SW"
BASE COUNT          442 a         1082 c          930 g          455 t
ORIGIN      
        1 cgacccgaag ggcgtgatga tcccgcgaac gtaaccggtc gcggcatacg caccgtccgc
       61 gtcgggcatc gacaaccgcg acgtgcccag cagcgcccgt agtttcggcc acgagaactt
      121 ccgatccccg ggtacgagca cgaacaggaa atccccgtcc ccgcgccgca ccacgagcgt
      181 cttgacgatg tcagccggcc gcaccccacg cgccgccgca gcctccgcca ggctcccgac
      241 cggcccgtgt cgcgtgacct cgtgcgcgac cccggccgcc gacaacgcgg ccaacgctcg
      301 cgcctcaccc tcgctcgtgc ccaccatcgc cccagggtat gccgcgtcgc ggtgccgccg
      361 tcgacgacgc agccgccgcc gtcgacgccg acgaagggtt taaaaggggg caggtagtcc
      421 cggttgtcac atctggacgc ctggcagtgt caggatggag gctgtcctcg acgggccacg
      481 tggtggtcga ggcaccgtcg agcacgatcg agtcgatcga caaggagtgt cgtgtcgtcg
      541 ttctgggccg agcacgtgtg gctgccgtcg ggcttggccc gcggcgtgcg cttggtcgtg
      601 gacgatggtc ggttcgtcgt cgtcgagccc cgagcgaccc gccaccccga ggatgtcaaa
      661 ctccgcggtg tcgtctttcc cgggttggcc aatgcccata gccatgtctt ccagcgggtc
      721 ctgcgcggac gcacccagtc tgacgcggac aacggaatca cttggcgcgc aaagatgtac
      781 gacgtcgctg aacgcctcaa ccccgatctc tatctcgctc tggcccgggc caccttcgcc
      841 gagatggcgc tggccggcat caccgtggtc ggcgaatacc actacctgca ccacggtccg
      901 ggcggctcgc gctactccga ccccaacgcc atgggcgctg ccctggccag ggcagcgcac
      961 gaggtcggga tccgactgac cctgctggac accctctacc tgcagggtgg gctcacctcc
     1021 gaggggcacc tgccgctcga cggtgtgcag cagcggttca gcgacggcac cgtcgaggcc
     1081 tgggccgccc gcaccgcccg catcgagcag accgaattgc ttcgtcgggg cgcggcgatc
     1141 cactcgatca gggccgtgtc gcgcgaggac gccgcctacg ccgcgcaact cgtcggggac
     1201 cgtccgctgc acatgtatgt ctccgagcag cccgccgaga acctcgcctg ccagatgtat
     1261 tacggcacca ccccgaccga actcctcgcc gacatcggcg tgctcgggtc ggagttcacg
     1321 gccgtccacg cgacccacct cactgagcgc gacatcagcc tcctggccga ccacgagtgc
     1381 caggtggtcg tctgcccgac gtccgagcag gacatggccg atggcgtccc ggcggtccgc
     1441 aggctcctcg acgccggggt cccggtcggc atcggcaccg accaacacgc cttcatcgac
     1501 ccgttcgtcg agatgcgtga gcttgagatg cacgagcgcc tcggcagcgg cgagcgtggc
     1561 cggatctcga cctcccagct cctgcgctgc gggtcggtcc acggctatca gagcctgggt
     1621 tggtacgacg gcgggtccat cgcccccggt ctggtcgccg acttcatcac cgtggatctc
     1681 gagtccaccc gcaccgcagg cagcaaggcc gccgaggtca tctacaccgc gagcgccgcg
     1741 gacgtcctca ccgtcgtcgt cggcggtcgg gtcatcgtcg agaacggcca gcatgcgttc
     1801 ggcccggtcg ccccgctcat gcgtgaggcg ctgtcgttga tccgcgacgc gagctgagcg
     1861 cgcgcggcat acccgatccg gtatgccgcg tcagccggtc gcgccgtcgg ccttgaccaa
     1921 cgcggcgggc aggtgaccag ccgacttcgc gtagtactgg gcggccttgc gggtcgccat
     1981 gacccgggcc gcactcacgg ccagcccggt gagggcggcg aagaggacgg cctctttcca
     2041 ggtggtgtcc tcgggatggt tggggtcgtc gggggccggc ttgccggagg cgagctccca
     2101 gcccttggtg acgagcttct gggcgacggt cgccgccagc actgccgagc cggtgcccag
     2161 caccttccac accatcggac ccatcccgca cctcctgctc caccgggcca ctcgccaacc
     2221 cgtgtcgttc gcctcgtccg actctggcac ggactcggag taaagtcatc atcgccacga
     2281 caggggagcg ccccagcgct gagagtgcgg tccgccgcag accctcgaac ctgctccggt
     2341 tagcaccggc gaagggagtc gagtctcacc cgtcgacctt cgatgggtcg acggcccctc
     2401 ctgaaccagg aggagatgtc agtatgccgt tcgcccaccc gcgcgtccgg gttccgttgc
     2461 gcaggagtcc cgtccgcagg gatcccttgc gcaagaaccg tttcgcgatc cccggcgccg
     2521 ttcttgcggt cgccctcact gcgtcgctct cggcctgttc gctgagctcg acgaccaccg
     2581 ccacagcgcc cgcaacgacg tcggggaccg cgaccgcgtc ccaggtcagc agcccgtcga
     2641 gcacggccgc accccggtgc ggcgccgtaa ccgaggtcct gctggtcagc cacgactcgt
     2701 tcgggctgtc cgacgagttg accgcggcct tcaccaaggc gaccggctgc acgatcaagg
     2761 tgctggccag cggcgacgcg ggcgagctga ccaacaaact cgccctcacc aaggcctcgc
     2821 cgatcggcga cgccgtcttc gggatcgaca acaccttcgc cagcccattc ttcttccgat
     2881 tggagttgac tctgccacca aattcctgg
//
LOCUS       DAOMZY010000403         2905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_33842_length_2905_cov_6.381053, whole genome shotgun sequence.
ACCESSION   DAOMZY010000403 DAOMZY010000000
VERSION     DAOMZY010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33842_length_2905_cov_6.381053"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PLK64_16105"
     CDS             <1..794
                     /locus_tag="PLK64_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HPV81318.1"
                     /translation="VDEVGPDSPLGLTVGDRVATLVSLSLTPLTITDGLRRWDGRSEQ
                     VPAAGHAVLFERSIAAVIPDDLDPRLSLMVMDVCGAPALVSRVVGEYAARRVAGTGAG
                     EESSAPSVLVLGGAGKSGSLSLAAAREAGAGRLVAVVPNDREAQVLRPTGLADEVVIA
                     DARSPMGLLAAVQAAGGPCDVTVVCVDVPGCEQPAILATAQEGTIIFFSMATSFAAAA
                     LGAEGLAADVRMLVGNGFVPGHAAYAMGLVRRNAAVRGLFESRIE"
     gene            896..2467
                     /locus_tag="PLK64_16110"
     CDS             896..2467
                     /locus_tag="PLK64_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HPV81319.1"
                     /translation="MSTTPLIHLDAGQVRQARSLARRAGAPVVKLAKAHTTVSVERAT
                     LRLAGLTGADHEGQPWVNHLVDAVRGQVGLEHGVTTPVFDALRAGAAPDLLTLAQQSA
                     AGSVTFRLPEGRDLKATQRAARTAVARGIRTIDAQRAVRDRLIRRHGDPKQTPWIYLI
                     VATGDIYEDIPQAQAAAREGADVIAVIRSTGQSLLDFVPEGATHEGFAGTYATQENFR
                     LMRAALDETSREVGRYVRLTNYASGLCMPEIATLAGLERLDMMLNDSMYGILFRDINP
                     IRTFVDQRFSRQIHARAGIIINTGEDNYLTTADAVDAAHTVTVSQLLNEYFAKEAGLE
                     DWQLGLGHAFEINPEVPESFRLELAHALLARTLFPDAPLKWMPPTKHMTGDVFRGYLL
                     DGFFNLAGTMTGQGILLVGMMTEAVVTPFLSDRDLALQNVRYVLDAAGGLREDFHPPR
                     DGFIQTRSRHVLGEAVDLLDRIATDGLLDAIADGTFGLMKRPADHGKGLDGVIVRAPD
                     YDNPAVTLLETGATR"
     gene            2464..>2905
                     /locus_tag="PLK64_16115"
     CDS             2464..>2905
                     /locus_tag="PLK64_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HPV81320.1"
                     /translation="MTNRKHLRETPRKVTPQIVRPYGDTTGDGMVQMSFTLPLPHDKR
                     AEGAALQLAKKMGMDPALVVHAKPIGPDFTFFVVYGSSNHLVDLSQVHVQEREFPLLT
                     PKEVNLAVKRGLRRPLVVVGACIGTDAHTVGIDAILNIKGFAGEK"
BASE COUNT          449 a         1061 c          940 g          455 t
ORIGIN      
        1 cggtggacga ggttggcccc gacagccctc tcgggctcac cgtcggtgac cgggtcgcga
       61 cgctggtgtc gctctcgctc accccgctga cgatcaccga cgggctgcgc cgatgggacg
      121 gccggtccga gcaggtcccg gccgccggtc acgccgtcct cttcgagcgc tcgatcgccg
      181 ccgtcatccc ggacgacctc gacccgcgcc tctcgctcat ggtcatggac gtctgcggcg
      241 cgcctgcgct cgtgtcccgc gtggtggggg agtatgccgc tcgccgcgtc gccggcaccg
      301 gtgccggtga ggagtcgtcg gctccgtcgg tcctcgttct cggcggagca ggcaagtctg
      361 gctcactgtc cctggccgcc gcgcgtgagg ctggggccgg ccgactggtt gccgtcgttc
      421 cgaacgaccg tgaggcgcag gtgctgcgtc cgacggggct ggccgacgag gtcgtcatcg
      481 ccgatgcccg ctcgccgatg ggcctgttgg ctgcggtgca ggcggcgggt ggaccgtgcg
      541 acgtcaccgt ggtctgcgtc gacgtgcctg gctgcgagca gccggccatc ctggcaaccg
      601 cacaggaggg gacgatcatc ttcttctcca tggccacatc cttcgccgcc gcggcgctgg
      661 gcgccgaagg gttggccgcc gatgtgcgga tgctcgtggg caacggcttc gtgcccgggc
      721 acgcggcata cgcgatggga ctggtccgtc gcaacgcggc ggtgcgcggc ctgttcgagt
      781 cccgcatcga gtgagccgac gccaaggatg atctccacga tgcccttgga ccgtcagatc
      841 tccggcgaga ttgtcgtttc gccggaaata tgcccgtctc tgttacgctg agaccgtgtc
      901 gacaacccct ctgatccacc tggatgccgg ccaggtccgc caggccaggt cactcgcgcg
      961 ccgcgctggc gcaccagtgg tcaagctcgc caaggcgcac accacggtct cagtggagcg
     1021 ggcgaccctg cgcctggccg ggctcaccgg agccgaccac gagggccagc cgtgggtcaa
     1081 ccatcttgtc gatgccgtgc gtggccaggt cgggcttgag cacggtgtca ccactccggt
     1141 cttcgacgcg ctgcgagcag gggccgctcc tgacctgctc accctggctc aacagtcggc
     1201 cgccggctca gtgacgttcc ggctgccaga aggccgcgac ctcaaggcca cccagcgggc
     1261 cgcacgcact gcggtggcac gcggcatacg caccatcgac gcccaacgcg cggtccgcga
     1321 ccggctgatc cgtcgccatg gtgacccgaa gcaaaccccg tggatctacc tcatcgtcgc
     1381 caccggcgac atctacgagg acatcccgca ggcgcaggcc gcggcccgag agggagccga
     1441 tgtcatcgcg gtcatccgct cgacagggca gagcctgctc gacttcgtgc ccgaaggcgc
     1501 cacgcacgag ggtttcgcgg gcacctacgc aacccaggag aacttccggc tcatgcgggc
     1561 cgccctcgac gagaccagcc gcgaggtggg ccgctatgtc cggctgacca actacgcgtc
     1621 cggcctgtgc atgcccgaga tcgccaccct cgcggggctc gagcggctgg acatgatgct
     1681 caacgactcg atgtacggca tcctcttccg cgacatcaac ccgatccgca ccttcgtcga
     1741 ccagcgcttc agccggcaga tccatgctcg cgccggcatc atcatcaaca ccggcgagga
     1801 caactacctc acgaccgccg acgcggtcga cgccgcgcac accgtcaccg tgagccagtt
     1861 gctcaacgag tacttcgcca aggaggcggg gctcgaggat tggcaactgg ggcttggaca
     1921 tgccttcgag atcaaccccg aggtccccga gagtttccgg ctcgagctgg cccacgcgct
     1981 gctcgcgcgg accctcttcc cggacgcccc gctgaagtgg atgccgccga ccaagcacat
     2041 gaccggcgac gtgttccgcg gctacctgct cgacgggttc ttcaaccttg ccggcaccat
     2101 gaccggccag ggcatcctgc tcgtcgggat gatgaccgag gccgtggtca ccccgttcct
     2161 ctccgaccgt gacctggcgt tgcagaacgt ccgctacgtc ctggacgccg ccggggggct
     2221 gcgcgaagac ttccacccgc cgcgagacgg cttcatccag acccgttcac gccacgtcct
     2281 gggcgaggcc gtcgacctgc tcgaccggat cgccaccgac gggttgctcg acgccatcgc
     2341 cgacggcacc ttcgggctga tgaagcggcc ggctgaccac ggcaagggcc tggacggcgt
     2401 gatcgtccgc gcgcctgact acgacaaccc agcggtcacc ctgcttgaga cgggagccac
     2461 gcgatgacca atcgcaagca tctgcgcgag acgccccgca aggtcacccc gcagatcgtc
     2521 cgcccctatg gcgacacgac tggggacggg atggtccaga tgtccttcac cctgccgctg
     2581 ccgcacgaca agcgagccga gggtgccgcc ctccaactgg ccaagaagat gggcatggac
     2641 ccggccctgg tggtgcacgc caagccgatc ggccccgact tcaccttctt cgtcgtctac
     2701 ggctcgtcca accacctcgt cgacctcagc caggtccacg tgcaagagcg cgagttcccc
     2761 ctgttgaccc ccaaagaggt caacctcgcc gtcaagcggg gcctgcgccg acccctcgtc
     2821 gtcgtcgggg cctgcattgg caccgacgcc cacaccgtgg ggatcgacgc catcctcaac
     2881 atcaaggggt tcgcggggga gaagg
//
LOCUS       DAOMZY010000404         2882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_34258_length_2882_cov_5.872657, whole genome shotgun sequence.
ACCESSION   DAOMZY010000404 DAOMZY010000000
VERSION     DAOMZY010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34258_length_2882_cov_5.872657"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PLK64_16120"
     CDS             complement(<1..288)
                     /locus_tag="PLK64_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017883558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPV81321.1"
                     /translation="MANDDAARRAELVLLRKVRDRIDRDYAQPLDVDALARGAHLSAG
                     HLSRAFKAAYGESPYSYLMTRRIERAKTLLRRGDLSVTDVCFAVGYGSLGTF"
     gene            425..823
                     /locus_tag="PLK64_16125"
     CDS             425..823
                     /locus_tag="PLK64_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018780112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPV81322.1"
                     /translation="MAIARFPVVVLDCPDPKALAEFYGALLGWEVQRGDGDSAGDSGG
                     DSDDGTWWQIRADYGDSMAFQAVEGYRAPQWPGQEHPQQMHIDVVVDDLDVAEAAVLE
                     LGATKHAHQPGTTFRVFLDPAGHPFCLCSS"
     gene            complement(844..1524)
                     /locus_tag="PLK64_16130"
     CDS             complement(844..1524)
                     /locus_tag="PLK64_16130"
                     /inference="COORDINATES: protein motif:HMM:NF022589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HPV81323.1"
                     /translation="MFWTVLGEVLTPGLGAALSPMLIVAAIVLGSAPSGPRKVFTFST
                     GVFVVMAVIGMLVVLGADKAEVATVPSRSEGGHILGLAVGILLCGMAWLTYKNRPTNG
                     EESSSVKRLNSLDALPHPRIFVMGMVLGLVNVKNIPLEISVGTNVVQTGAGVPLSLIG
                     MLVFSAMACSTLVTPGVIVLAAPEKSTRILGHARKVLIANNTAIMIVLYLMLGTNLIS
                     KSLGPLFA"
     gene            complement(1621..1992)
                     /locus_tag="PLK64_16135"
     CDS             complement(1621..1992)
                     /locus_tag="PLK64_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010550244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV81324.1"
                     /translation="MSDFAPDPFNPACPSRRLLDRIGDRWTVLIVVALDPGPQRFGAL
                     ASRVGGISQKMLTQTLRSLERDGFVTRTAYPEIPPRVEYALTPLGRSLLGPLRALEEW
                     AVTNLPAVASHQQGYAAAAAD"
     gene            2108..2758
                     /locus_tag="PLK64_16140"
     CDS             2108..2758
                     /locus_tag="PLK64_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HPV81325.1"
                     /translation="MARITVLGGSGYAGGAIVAEAAKRGHEVTSVSRTAVAAPVAGVE
                     YAVGSALDPAVLDAVTAGRDVVVVALSPRGDMAGKLEGVVEDLIGRLAGGATRLGYVG
                     GASSLLVSEGGPRLWDVSQEHIPADVKPEIETGLRAFELVKASPATLDWFYVSPPADF
                     GSWFNTESKGTYVLGGDVLQRDADGKSTISAADLALAILDEIETPTHHRGRFTAIA"
BASE COUNT          468 a          897 c         1051 g          466 t
ORIGIN      
        1 gaaggtgccg agcgagccgt agccaacggc gaaacacacg tcggtcaccg acaggtcgcc
       61 gcgccgcagc agggtcttgg cccgctcgat ccggcgggtc atcaggtagc tgtagggcga
      121 ctcgccgtag gccgccttga aggcgcggct gaggtgaccc gccgacaggt gcgcgccgcg
      181 ggcgagagca tcgacgtcga ggggttgcgc gtagtcgcgg tcgatccggt cgcggacctt
      241 gcgcaggagc acaagctcgg cgcgccgggc ggcgtcatcg ttggccaccg ggcaagcgtc
      301 tcaggaaacg tggtttgctc ggcggagggg ggccgtgagg gggagtgatg gggtgtgaaa
      361 gaacctgtac ttcgggtacg gaattgaagg cagagtcgtg cggccggcgt tggtagtgtc
      421 gggcatggct atcgctcgtt tccctgtggt cgtgttggac tgccccgacc cgaaggccct
      481 ggccgagttc tatggggcgt tgttggggtg ggaggtccag cgcggtgatg gcgacagcgc
      541 cggcgacagt ggcggcgaca gcgacgacgg cacctggtgg cagatccgcg cggactacgg
      601 cgactcgatg gcgttccagg ccgtcgaggg ttatcgggcg ccgcagtggc ccggccagga
      661 gcacccccag cagatgcaca tcgacgtggt cgtcgacgat ctcgatgttg ccgaggccgc
      721 cgtgctcgag ttgggcgcga ccaagcatgc gcatcagccc ggcacgacct tccgggtctt
      781 cctcgacccc gccggccacc cgttctgcct ctgctccagc tgagcccgtt cactccgccg
      841 gcgtcaagcg aacagtgggc cgagtgactt gctgatcagg ttcgtgccca acatgaggta
      901 gagcacgatc atgatggccg tgttgttcgc gatgaggacc ttgcgcgcgt ggccgaggat
      961 gcgagtggat ttctctggtg ccgcgaggac gatgacgccg ggggtcacga gggtgctgca
     1021 ggccatcgcg gagaagacca gcatgccgat caggctgagc ggcacgcctg cgcccgtttg
     1081 aacgacgttc gtgccgaccg agatctccag cggaatgttc ttgacgttga ccaggccgag
     1141 caccattccc atgacgaaga ttcgcggatg cggcaaggcg tccaaggagt tcagtcgctt
     1201 gaccgaggac gactcttcgc cgttggtggg ccggttcttg taggtcaacc acgccatgcc
     1261 gcacaagagg atgcccacgg ccagtccgag aatgtgcccg ccctccgacc gcgacggcac
     1321 cgtggcgacc tcagccttgt ctgctcccaa caccacgagc atcccgatca ccgccatgac
     1381 cacgaagacg cccgtcgaga aggtgaacac cttgcgcggg cccgatggcg cgctcccgag
     1441 gacgatcgca gccacaatca gcatcgggct caaggcggca cccaggccag gggtgagcac
     1501 ctcgccaagg acggtccaga acacgggtgt cgctccctcg aactatgacc aacccttcga
     1561 tggtagccac gtcagcaaga ccccgagaag ggctcggacg cagcggcgag gggcccgcgc
     1621 tcagtcggcg gcagcggcgg catacccctg ctggtggctg gcgacagcgg gcagattggt
     1681 gacggcccac tcctccagcg cgcgcaacgg cccgagcaag gagcgcccga ggggggtgag
     1741 cgcatactcc acacgcggcg ggatctccgg gtatgccgtg cgcgtcacga agccatcgcg
     1801 ctctagcgag cgcagggtct gggtgagcat cttctgcgag atgcccccga cgcgggaggc
     1861 gagcgctcca aaccgttgcg ggccggggtc gagcgcgacg acgatcagga cggtccaccg
     1921 gtcgccgatc cggtcgagca gccgccgcga cgggcaggcc gggttgaacg gatctggtgc
     1981 gaaatcgctc acgatgccgg ctcttctctg cggggctcgg gtcacgcacc ccaaagtgcg
     2041 tactcccggg atggctttac atgacttatg gtaacccagt aacccacaga gagtattgga
     2101 gagagtcatg gcacggatca cggttctggg cggcagcggc tacgcgggcg gggcgatcgt
     2161 cgccgaagcc gccaagcgcg gacacgaggt cacgtcggtc agccgcaccg ccgtcgcggc
     2221 gcccgtcgcg ggcgtggagt atgccgtggg gtcggcgctc gacccggccg tccttgacgc
     2281 ggtgacggcg gggcgggacg tggtcgtcgt tgccttgtcg ccgcgcgggg acatggcggg
     2341 caaactcgag ggcgtcgtgg aagacctcat cggtcggctg gctgggggtg cgacgcggct
     2401 ggggtatgtc ggcggcgcgt cgtcgctgct ggtgtcggag ggtgggccga ggctgtggga
     2461 tgtctcccag gagcacatcc cggccgacgt gaagcctgaa atcgagacag ggctgcgggc
     2521 cttcgagttg gtgaaggcgt ccccggcgac tctggactgg ttctatgtca gcccgcctgc
     2581 tgacttcggg tcgtggttca acacggagtc gaaggggacg tatgtgttgg gcggcgacgt
     2641 gctgcagcgc gacgccgacg gcaagtcgac catctcggct gccgacctcg cgctggcgat
     2701 cctcgacgag atcgagactc cgacgcatca ccgtgggcgc ttcaccgcga ttgcctgatt
     2761 cggtctggcg gcccggctac gcggccgggc cgcctgagtc gaaccaaggg tctgcctcgg
     2821 ggttggggtc gcagcggtcc cggtgatcag ttgctcggcg gtcgttcccg tttcccgagg
     2881 ct
//
LOCUS       DAOMZY010000405         2879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_34306_length_2879_cov_5.002125, whole genome shotgun sequence.
ACCESSION   DAOMZY010000405 DAOMZY010000000
VERSION     DAOMZY010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34306_length_2879_cov_5.002125"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="PLK64_16145"
     CDS             <1..958
                     /locus_tag="PLK64_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HPV81326.1"
                     /translation="WHETARETGARIVHSCGFDSIPSDLGVLVTADTVAADGAGELTE
                     TVLSVVSMRGGVSGGTIDSMRQQAILMRADAAVRAIVADPYGLSPDRPAEPRSRGGEA
                     GAEADAGAGAGAGAGALAKTVWTSAVRLVRRSPIRRDPVTGHWTGPFVMAGFNTRIVR
                     RSNALLGWGYGRAFRYREVVDFGNSAKAPVLATGMSAGLLGLAGAMAFEPTRAVVDRF
                     LPKPGEGPSEENQANGRFRMVIRTTTTTGAAYRTKVGADRDPGYSGTAVMLGESALAL
                     ALDGDLLPGGGGVLTPATGLGSVLVDRLVAQDFTFDCERVDS"
     gene            complement(1257..1811)
                     /locus_tag="PLK64_16150"
     CDS             complement(1257..1811)
                     /locus_tag="PLK64_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012802312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPV81327.1"
                     /translation="MAAPITYVLVDGENVDATLGTSILMRRPASEERPRWERLLSFAE
                     TTWSQPARGLFFLAANADLPMAFVQALLAIGYRVIPLRGAPGEKVVDLAIQRTLEEIG
                     RRSGDVILVSNDGDFIPQVSDLCSTRRVGIIGFTEFRNQGLTALSQRGVELFDLEYDV
                     QAFNSRLPRVRIIPIEEFNPADFL"
     gene            complement(1821..>2879)
                     /locus_tag="PLK64_16155"
     CDS             complement(1821..>2879)
                     /locus_tag="PLK64_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPV81328.1"
                     /translation="DVINLVSKQFNADGTGWFFNGPRIHEFVAEMHENAWGGKQLITV
                     GEAPGATVDDAVRFTDPARGEFDMVFQFEHMGVDHGMGGRYDIRPVDWVRLKSIMARW
                     QDGLAEVGWNSLYWCNHDQPRPVSRYGAGSTAYREVSAKALATVLHLHRGTPYVYQGE
                     ELGMVNYPFTSLDQLDDVESRNWSAEAVGKGMDPAVALAAVNVQSRDHARTPMQWDAS
                     EHAGFTTGTPWLPVNPAYREINAAAQVGDPESVFAHYQGLIRLRRELPVVVEGRFELL
                     LADHPSVYAFTRALEGDELLVVANLSGESVEVALPDGWEDAEVVISSGDSDDSGMPPA
                     HLGVLAPWQAWVRRRQVG"
BASE COUNT          452 a         1010 c          976 g          441 t
ORIGIN      
        1 ctggcacgaa accgctcggg agacgggggc gcggatcgtg cattcgtgcg ggttcgactc
       61 gatcccgtcc gacttggggg tgttggtaac ggccgacacc gtggctgccg atggggcggg
      121 cgagctcacc gagaccgtgc tgtcggtggt gtcgatgcgc ggcggggtga gcggcggcac
      181 gatcgactcg atgcgccagc aggcgatcct catgcgggcc gacgctgcgg tccgggcgat
      241 cgttgccgat ccgtacggct tgagccccga ccggcccgcc gaaccgcgct cccgtggtgg
      301 tgaggccgga gccgaggctg acgccggggc tggggccggg gctggggccg gggcgctagc
      361 taagacggtc tggacgtcgg cggttcgact ggttcggcgg tcaccgatcc gccgcgaccc
      421 ggtgaccggg cactggaccg ggccgttcgt catggccggg ttcaacacgc ggatcgtccg
      481 gcgcagcaac gcgttgctgg gctgggggta tggccgggcg ttccgctatc gcgaggttgt
      541 cgacttcggc aacagcgcca aggctccggt gctggcgacc gggatgtcgg caggtttgct
      601 cggcttggcc ggggcgatgg cgttcgagcc gactcgggcg gtggtcgacc ggttccttcc
      661 caagcctggc gagggaccga gcgaggagaa ccaggcgaac ggccggtttc ggatggtgat
      721 ccgcacgacg acgaccacgg gcgcggcata ccggacgaag gtgggggccg atcgggaccc
      781 tggctacagc gggaccgcgg tcatgctcgg cgagtcggcg ctggccctgg cgctggacgg
      841 tgacctgctg cccggaggcg gaggtgtgct gacgccggcg accggcttgg gttcagtgct
      901 cgtcgaccgg ctggtggcac aagacttcac cttcgactgc gagcgcgtcg actcctgacc
      961 cagcctgatc tcgcccaagc ctgccctacc tgacctgccc gacccctgcc gaccctgccc
     1021 gacccctgcc gaccctgccc gccccaaaac cacacacccg ccgacgcttt cttaacgccc
     1081 aggcctcgat cgccccaaga tcctcacagc tgccagggtt ctgggcgttg ggtatgccgt
     1141 tcgcccccga attcaccaca ctcgccggcg atttcccggc cccacgaacc ccagcccgcc
     1201 cgacgaaccc cccgcaaccc gacgaacccc agccaaccag cctcagccag ccagcctcag
     1261 aggaagtcgg ccgggttgaa ctcctcgatg ggaatgatcc gcacccgggg aaggcgcgag
     1321 ttgaaggcct gcacgtcgta ctcgaggtcg aacaactcaa cccctcgctg actcagagcg
     1381 gtgagcccct ggttgcggaa ctcggtgaac ccgatgatcc cgacccgccg cgtcgagcac
     1441 aggtcgctca cctgcgggat gaaatccccg tcgttgctga ccaggatgac gtcgcccgat
     1501 cggcggccga tctcctcgag ggtccgctgg atggccagat cgacgacctt ctcgccgggg
     1561 gcgccgcgca acggaatgac gcgatagccg atggccagca gcgcctgcac gaacgccatc
     1621 ggcaggtcgg cattggcagc gaggaagaac agcccgcggg ccggctgaga ccacgtggtc
     1681 tcggcgaagc tcagcagtcg ctcccagcga ggacgttctt cacttgccgg acgccgcatg
     1741 aggatcgacg tccccaacgt cgcgtcgacg ttctcgccat caacaaggac gtacgtgatc
     1801 ggcgcggcca tgcaccgaac ctagccgacc tgtcgccgcc gcacccacgc ttgccaaggg
     1861 gcaaggacac cgaggtgagc gggcggcata ccggagtcgt cagagtctcc cgacgagatg
     1921 acgacctccg cgtcctccca gccatccggc agcgcgacct cgaccgactc cccgctgagg
     1981 ttcgcgacga ccaacaactc gtcgccctcc agcgcccgcg tgaaggcata gacgctcggg
     2041 tggtcggcga gcagcagctc gaaccgcccc tcaacgacca ccggcagctc gcgccgcagc
     2101 cggatcagcc cctggtagtg ggcgaagacc gactccgggt cgcccacctg cgcggccgcg
     2161 ttgatctccc ggtatgccgg gttgaccggc agccacggcg tccccgtcgt gaaccctgcg
     2221 tgctcggacg cgtcccactg catcggcgtg cgcgcgtgat cgcggctctg gacgttgacc
     2281 gcagcgagcg ccaccgcagg gtccatgccc ttgcccaccg cctccgccga ccagttgcgc
     2341 gactcgacgt cgtcgagctg atcgagcgag gtgaaggggt agttgaccat ccccagctcc
     2401 tccccctggt agacgtaggg ggtgccgcgg tgcaggtgca gcaccgtcgc gagcgccttc
     2461 gccgagacct cgcggtatgc cgtgctgccg gctccgtagc gcgacaccgg tcgcggttgg
     2521 tcgtggttgc accagtagag gctgttccag ccgacctcgg ccaggccgtc ctgccagcgg
     2581 gccatgatcg acttcagccg gacccagtcg acggggcgga tgtcgtagcg gccgcccatc
     2641 ccgtggtcga cgcccatgtg ctcgaactgg aagaccatgt cgaactcgcc gcgcgccggg
     2701 tcggtgaacc gcaccgcgtc gtcgacggtg gcgcccgggg cctccccaac ggtgatgagt
     2761 tgctttccgc cccaggcatt ttcgtgcatc tcggcgacga attcatggat ccgcggcccg
     2821 ttgaagaacc acccggtgcc gtcggcgttg aactgcttgg agacgaggtt gatgacgtc
//
LOCUS       DAOMZY010000406         2846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_34913_length_2846_cov_6.336080, whole genome shotgun sequence.
ACCESSION   DAOMZY010000406 DAOMZY010000000
VERSION     DAOMZY010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34913_length_2846_cov_6.336080"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="PLK64_16160"
     CDS             complement(<1..681)
                     /locus_tag="PLK64_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81329.1"
                     /translation="MADAPETHQTTSAVVEAAFEAQAPGIKRAAVIGGLASASGIALT
                     ATSGWLIVAASHQPVILTLLAVIVAVRTFGIARPVLRYAERVRSHDSALDLLARRRVA
                     AYQALVPLTPARLGRRSRADVLTTAVEDLEDLVFAKVRVVVPVLGLAVAAVLTVLALG
                     LFVPAGSLVLVAVLLAQVGVSALGRRMELAAQQDWLRARAEVARTATLVTGYAAQLRG
                     IGAGPQALG"
     gene            complement(674..2266)
                     /gene="cydD"
                     /locus_tag="PLK64_16165"
     CDS             complement(674..2266)
                     /gene="cydD"
                     /locus_tag="PLK64_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HPV81330.1"
                     /translation="MRPIDPRVFQALPEARRIMAGLGALGVALGVAAVAQAFAVSAVV
                     VAVVRFGMPGAPGLLTALLALAAVFAVRATLGALNEAYAARAGIRVSAALRERLLARW
                     LAAPVDRMPPPATASTLATQGVTSVEPYVARFLPAMVGAVVLPVFVVVTIVFLDWVSA
                     LIVVLTVPLLPVFAALIGRATQDRTERRWQALTALSGHFLDVVRGLPTLVTYGRAERQ
                     AESIRTVSEAHRAATVRTLRVAFLSSAALELLATISVAIVAVTVGLRLTYGTMELGAG
                     LACILLAPEAYWPIRRVGTEYHAAADGVAALGAALDELADAPAGVSAPAIPTRFGTDG
                     VSYRYPGTERVVVDDVTLLLPGAGLVTITGPSGVGKSTLLELLAGLREPTAGVVSAPE
                     AHLVGQRPLLVTGSLRTNLTLGLDTPPNDDALWSALDEVGLGDDLRALAGGLDAPLGD
                     DGFGLSAGQRARLALARAILSEAALVLLDEPTAHLDERSAGLAHNAIQRLARTRSVVV
                     VTHRPELVELADAEVRLGGDRG"
     gene            complement(2307..>2846)
                     /locus_tag="PLK64_16170"
     CDS             complement(2307..>2846)
                     /locus_tag="PLK64_16170"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HPV81331.1"
                     /translation="VVPLIGGLTFVGLFLTHGAFFIALKTDGPIRADARALGIKTGLV
                     TAVLAVVLLLAINVRHGSLVSWIGTVVAAVALLGGIFMATKGREGWAFLGTFVCIAFA
                     VVAYFGALFPNAMPTTLADGADLTLAAASSTPKTLTIMTWAAAVFTPIVLLYQGWTYW
                     VFRKRISTKNIPAPVTVGK"
BASE COUNT          507 a          985 c         1026 g          328 t
ORIGIN      
        1 accgagtgcc tgcgggccgg cgccgatgcc acggagctgc gcggcatacc cggtgacgag
       61 agtcgcggtg cgcgccacct cggcgcgagc gcgcagccaa tcctgttgtg ccgcaagctc
      121 catacgacgc ccaagcgcgc tgacaccgac ctgcgcgagc aggacggcga caagcaccag
      181 cgacccggcc ggcacgaaga gtcccagagc caagacggtg agcacagccg cgacggccag
      241 cccgagcacc ggcacgacga cccgcacctt ggcgaacacc aggtcttcga ggtcctcgac
      301 cgccgtcgtg aggacgtcag ctcgcgagcg tcgccccagc cgggccgggg tgagcggcac
      361 gagtgcttgg tatgccgcca cccgccgccg cgcgaggagg tccagggcgc tgtcgtgtga
      421 gcgcacccgc tcggcgtaac gcagcaccgg gcgggcgatg ccaaaggtcc ggaccgcgac
      481 gatgacggcc aagagggtga ggatgaccgg ttggtgactc gcggcgacga tgagccagcc
      541 agaggtcgcc gtgagggcga tgcccgacgc cgaggcgagc ccaccgatga ccgctgcgcg
      601 cttgatgcct ggggcctgcg cctcgaacgc ggcctcgacg accgccgagg tcgtttgatg
      661 ggtttcggga gcgtcagcca cggtcgcctc ccaaccgaac ctcggcatca gcgagctcga
      721 ccagctcggg acgatgggtg acgacgacga ccgatcgggt ccgcgccaga cgctgaatcg
      781 cgttgtgggc cagccctgct gagcgttcgt ccaggtgtgc ggtcggctcg tcgagcagga
      841 ccaatgcggc ctctgacaag atcgcccgag ccaacgccag ccgggcgcgc tgacccgccg
      901 acagcccgaa accgtcgtcg cccagcggtg cgtcgagccc acccgccagc gctcgcaagt
      961 cgtcgccgag cccgacctcg tcgagcgccg accagagggc gtcgtcgttg ggcggcgtgt
     1021 ccagcccgag ggtgaggttg gtccgcagcg agccggtgac cagcagcggc cgctgtccga
     1081 cgaggtgcgc ttcgggggcg gacacgactc cggcggtcgg ctcccgcagg cccgcgagca
     1141 gttcaagcag cgtcgatttg ccgaccccgg acggcccggt gatggtcacc aagccagcac
     1201 ccggcaggag caacgtcacg tcgtccacaa cgacccgttc ggtgccgggg tagcgataac
     1261 tcaccccatc ggtcccgaac cgagtgggga tcgcgggagc cgacacacct gctggagcat
     1321 cggccaactc gtcgagggca gcgcccaggg cggcgacccc gtcagcagct gcgtgatact
     1381 cggtcccgac ccggcggatc ggccaataag cctctggggc aaggagaatg cacgccaagc
     1441 cagccccgag ctccatcgtc ccgtaggtca gccgaagccc caccgtcacc gcgacgatcg
     1501 ctaccgaaat ggtcgccagc aactccagcg cagccgacga caagaacgcc acgcgcaacg
     1561 tccgcaccgt ggccgcccga tgcgcctcgc tcaccgtccg gatcgactcg gcctgccgct
     1621 cggcccgccc ataggtcacc aacgtcggca gcccccggac gacgtcgagg aagtgcccgg
     1681 acagtgccgt gagcgcctgc cagcgacgct cggtgcggtc ctgggtagcg cgaccgatca
     1741 gtgcggcgaa gaccggcagc aacggcaccg tcaggacgac gatgagggcg ctcacccagt
     1801 cgaggaacac gatcgtcacc acgacgaaga ccggcaggac caccgccccg accatggccg
     1861 ggaggaatcg ggcgacatag ggctcgacgc tcgtgacccc ctgtgtcgca agggtgctcg
     1921 cggtggcggg cggcggcata cggtcgacgg gagcggcgag ccaccgggcc aggaggcgct
     1981 cacgcaaggc agccgacacg cgtatgccgg cccgcgcggc atacgcctcg ttgagggctc
     2041 ccagcgtggc gcggaccgcg aagacggccg cgagcgcgag gagggcggtg agcaggccgg
     2101 gggcacccgg cataccgaag cgaacgacgg cgaccacgac ggccgagacg gcgaaggctt
     2161 gggcgacggc ggcgacgccg agtgccacgc ccaacgcccc gaggcccgcc atgatgcggc
     2221 gggcctcggg gagggcctgg aagacccggg ggtcgatcgg tcgcacgggt tgatgctagg
     2281 tgagccgcga ggcggtcacc cgctcgtcac ttgccgacgg tgaccggggc ggggatgttc
     2341 ttcgtggaga tccgcttgcg gaacacccag taggtccagc cctggtagag caggacgatt
     2401 ggcgtgaaga ccgcggcggc ccaggtcatg attgtcagcg tcttcggtgt gctcgacgcg
     2461 gcggcgaggg tgaggtcggc gccgtcggcg agcgtcgtgg gcatcgcgtt cgggaacagc
     2521 gccccgaagt aggccacgac agcgaaggcg atgcagacga aggtgccgag gaacgcccag
     2581 ccctcacgac ccttggtggc catgaagatg ccgccgagca gggcgactgc cgccacgacc
     2641 gtgccgatcc agctgaccag cgacccgtgc cgcacgttga tggccagcaa caggacgacg
     2701 gcgaggaccg cggtgacgag gccggtcttg atgcccaacg cgcgggcgtc cgcccggatc
     2761 gggccgtcgg tcttgagcgc gatgaagaag gcgccgtggg tgaggaacag cccgacgaac
     2821 gtgagcccac caatgagggg cacgac
//
LOCUS       DAOMZY010000407         2821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_35392_length_2821_cov_5.415401, whole genome shotgun sequence.
ACCESSION   DAOMZY010000407 DAOMZY010000000
VERSION     DAOMZY010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35392_length_2821_cov_5.415401"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1199
                     /locus_tag="PLK64_16175"
     CDS             <1..1199
                     /locus_tag="PLK64_16175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPV81332.1"
                     /translation="PGDEWHAVLRPGEPMAPRTPWFVAPDVARGRIRLREFRDDDEHR
                     LPAERDDRAARFLTRGMPTRSRYAAWLTEQRSAAAMGDTVQWALADRHTDEVLGAIVL
                     TQLAHPRTRGTAVVGYWLLPQWRGQGLMTEGLDLAVDTAFAPKPEGLGLRALRAVADA
                     TNTESARVLRAAGFVSGGRERAAYAHVHEPPADAVTFDVLVSDDREAQRVRPLPIPVI
                     ETERLRLRPWRETDRPDPADDPDEAVRRFIPAGAAPGHADFDVWLARKRRFNDEGSLV
                     EWCLADRETDRALGSVGLFTRGDGPIDFNVEIGYFIFPTARGHRYVGEALPQVIEHAF
                     RPRSEGGLGVSRVHAGADTDNDASRAILRAAGLREWGHDRQAWRRADGSLSDGSYAEL
                     LVTDPR"
     gene            complement(1166..2602)
                     /locus_tag="PLK64_16180"
     CDS             complement(1166..2602)
                     /locus_tag="PLK64_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV81333.1"
                     /translation="MTTPSTERRTIHRAWWVALAVLGALVAAAGFRSSTGALIEPLEQ
                     EFGWSRTMTSGAVTANLVIYGLTAPFAAALMERLGLRRVIVAALLLVGIGSGLTTVMT
                     SPWQLWVLWGGFVGLGAGAMALVLGSVVANRWFESRRGLVTGLFSAASSTGQLVFLPA
                     IAALAAGAGWRYAALVVAGAAFAIIPVIVLVVADRPADVGVRPYGAAPESKPEPEPKS
                     KPESRPESVVTPTAVVSGRHTEDTGGGPAAAAPARIALQTLGEASHRLSFWLLAGTFF
                     ICGWSTNGLIGTHFIPAAHDHGMPPTTAAGLLALIGIFDIAGTVASGWLTDRVDPRLL
                     LVGYYVLRGLSLLVVPSVLGPGVEPSLFLFIVFYGLDWVATVPPTMALCRAHFGLERS
                     SIVFGWVFAAHMVGAGIAATFAGWVRQSSGDYLWAWLTAGILCLIAGFACLAIPRRAP
                     EGARSPGATPDDAGPSAGQRGSVTNSSA"
BASE COUNT          458 a         1027 c         1006 g          330 t
ORIGIN      
        1 atcccggcga cgaatggcat gccgtgctgc gccccggcga gccgatggcc ccgcgcaccc
       61 cctggttcgt cgcccccgac gtcgctcggg gccggatccg gctgcgagag ttccgcgacg
      121 acgacgagca ccggctgccg gccgaacgcg acgaccgcgc ggcccggttc ctcaccaggg
      181 gcatgcccac ccgcagccgg tatgccgcgt ggctcaccga acagcgctcc gccgccgcaa
      241 tgggcgacac cgtccagtgg gcgctggccg accggcatac cgacgaggtc ctgggggcga
      301 tcgtcctcac ccaactggcg catccgcgca cccgaggcac ggcggtcgtg gggtactggc
      361 tgctccccca gtggcgcggc cagggtttga tgaccgaggg cctggacctc gcggtcgaca
      421 ccgcgttcgc gccaaagccc gaaggcctgg ggctgcgggc cctgcgtgca gtggccgacg
      481 cgaccaacac cgagtcggct cgggtgctgc gtgcggccgg cttcgtcagt ggcggccggg
      541 agcgagcggc atacgcccat gtgcacgagc cccccgccga cgcggtcacc ttcgacgtgc
      601 tggtgagcga cgaccgggag gcccagcgcg tgcggccctt gccgattccg gtcatcgaga
      661 ccgagcgact tcgcctacgg ccgtggcggg agaccgaccg gcccgaccct gccgacgatc
      721 ccgacgaagc cgtgcgacgc ttcatccccg ccggtgcggc tcccgggcac gccgacttcg
      781 acgtgtggct cgcacgcaag cgccggttca acgacgaagg cagcctggtg gagtggtgtc
      841 tcgccgatcg cgagaccgac cgggcgctgg gcagcgtggg actcttcacc cgcggtgacg
      901 gaccgatcga cttcaacgtc gaaatcggct acttcatctt cccgacggcg cgcgggcacc
      961 gctacgtcgg tgaggcgctc ccccaggtca tcgagcacgc cttccggccg cgatcggaag
     1021 gcggcctcgg cgtgagccgc gtccatgcgg gggccgacac ggacaacgac gcctcacggg
     1081 cgatcctgcg cgccgccggg ctgcgcgagt ggggccacga ccggcaggcg tggcggcggg
     1141 ccgacggctc cctgagcgat ggaagctatg ccgaactatt ggtcaccgat ccgcgctgac
     1201 cggcggacgg ccccgcgtcg tccggggtgg caccgggcga tcgcgcgcct tcgggtgccc
     1261 ggcgcgggat cgccaggcag gcgaatccgg cgatgagaca gaggattcca gcggtcagcc
     1321 acgcccagag gtagtcaccg ctggactggc gcacccagcc cgcgaaggtc gccgcgatcc
     1381 cggccccgac catgtgcgcg gcgaacaccc agccgaacac gatgctcgag cgttccagcc
     1441 cgaagtgcgc ccggcacagc gccatcgtcg gcgggaccgt cgccacccag tccaatccgt
     1501 agaagacgat gaagaggaac aggctcggct cgacccccgg cccgaggacc gacgggacga
     1561 cgagcagcga caggccccgc aagacgtaat agcccaccaa cagcagccgc ggatcgaccc
     1621 ggtcggtcag ccagcccgag gcgaccgtcc cggcgatgtc gaagatgccg atcagggcga
     1681 gcaaccccgc cgcggtcgtc ggcggcatcc cgtggtcgtg agccgcaggg atgaagtggg
     1741 tgccgataag gccgttcgtc gaccaaccgc agatgaagaa cgtcccggcc aggagccaga
     1801 acgacagtcg gtggctcgcc tccccgaggg tctgcaaggc gatccgcgca ggtgcggctg
     1861 ccgcggggcc cccgcccgtg tcctcggtat gccgcccgct caccactgct gttggcgtga
     1921 ccacggactc gggcctggac tcgggcttgg acttgggctc gggctcgggc ttggactcgg
     1981 gcgcagcacc gtaggggcgg accccgacat cggccggacg atcggcgacc acgaggacga
     2041 tcaccggaat gatcgcgaag gctgccccgg cgacgacgag cgcggcatac ctccagccgg
     2101 ctccggccgc gagcgcagcg atcgcgggca ggaagacgag ttggccggtc gaggaggccg
     2161 ccgagaagag gccggtgacc aggccccgtc gggactcgaa ccaccggttg gcgacgaccg
     2221 acccgaggac cagggccatc gccccagcgc ccaacccgac gaacccgccc cagaggaccc
     2281 acaactgcca gggcgaggtc atcaccgtcg tcagcccgct gccgatcccg acgagcagca
     2341 gcgcggcgac gatgacccgg cgcaggccca ggcgctccat gagggcggcg gcgaacggcg
     2401 ccgtcaggcc atagatgacg aggttggcgg tcacagcgcc cgaagtcatg gtgcggctcc
     2461 acccgaactc ctgctcgagg ggctcgatga gcgcgcccgt cgaggaccgg aaaccagcgg
     2521 cggcgaccaa ggcgcccagc acggccaggg ccacccacca ggcgcgatgg atggtgcgac
     2581 gttcggtgga cggggtcgtc acgatcactc cttaggtcct ggcgaagccc gccggatgcg
     2641 cagggcagca gtctggccta cgacccgccg aatcgccgac ccggtttagg gggcagtgcc
     2701 cgggaccgac acgtcggtca gttcgagcca accggccagg gcggcgagct cgcgttccag
     2761 cgccgcggcg gcctcgggcg gcgccccggg ctcccaggtc agttgcggca cccgcaggac
     2821 c
//
LOCUS       DAOMZY010000408         2710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_37609_length_2710_cov_6.494915, whole genome shotgun sequence.
ACCESSION   DAOMZY010000408 DAOMZY010000000
VERSION     DAOMZY010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37609_length_2710_cov_6.494915"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /gene="pgi"
                     /locus_tag="PLK64_16185"
     CDS             <1..1154
                     /gene="pgi"
                     /locus_tag="PLK64_16185"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPV81334.1"
                     /translation="LTCRFISNIDPTDAAQKTAGLDPETTLVIVASKTFTTLETITNA
                     RLVRTWLLDSLAASGAIDGSDASQADAVAKHFVAVSTALDKVAAFGIDPANAFGFWDW
                     VGGRYSVDSAIGTAVAVAIGPERFAELLAGFHAVDEHFRTAPLERNVPALMGLLNVWY
                     VNHLDARSHAVLPYAQQLHRFAAYLQQLTMESNGKSVRWDGTPVTTTTGEVFWGEPGT
                     NGQHAFYQLLHQGTQLIPADFIAVATPAYPLTDGAADVHELFLANFFAQTAALAFGKT
                     ADEVRAEGTAEAIVPARVFSGNRPTTSIMAPALTPSVVGQLVALYEHITFTQGIVWGI
                     DSFDQWGVELGKQLAKKIEPAVAGDEVALGDQDASTQSLIRYYLAHRER"
     gene            complement(1180..2451)
                     /locus_tag="PLK64_16190"
     CDS             complement(1180..2451)
                     /locus_tag="PLK64_16190"
                     /inference="COORDINATES: protein motif:HMM:NF019436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81335.1"
                     /translation="MKASEAAEVGRVLGETLVGTARRSGQLSAAILDRGYGAAARIAG
                     PAHRDRIERVRTAHRQATTWAGQAVGRGLGAIGHGVARVGARFADGRPVVEEPGAYQA
                     VSVLNGLYGDRLQQRHSPLSLPMALRAEGRDVPLSRGGLHAAYPHATGLVVVFIHGLV
                     ETDDWWDIRSERNWGHPGESYSRRLRAEGAWTPVRVRYNTGLRVSNNGQHLDDLLDAL
                     VAGWPVPVCGLVLVGHSMGGLVLHSALAQASSDRQWPSLVRVTVTIGTPHQGAAIVRG
                     VARLTPVLARLPETRWLAEVISSRPAGMGDLGHGNLLADDWHDVDSGALTAPARTVPL
                     AGGIRHCVVIGTVPSKRDGLVAGWVGDLLVRPADAQVAASRGEVDPRDIRVVGGARHL
                     DLLNHPAVYVHLSEWLAFAARHSPAPEPSEG"
BASE COUNT          422 a          979 c          933 g          376 t
ORIGIN      
        1 ggctgacctg tcggttcatc tccaacatcg acccgaccga tgccgcccag aagacggccg
       61 gtctggaccc cgagacgacg ctcgtcatcg tcgccagcaa gaccttcacc accctcgaaa
      121 cgatcaccaa cgcccgcctc gtgcgcacct ggttgctcga ctcactggca gcgtctggcg
      181 cgatcgacgg gtcggacgcc tcgcaggccg acgccgtcgc caagcacttc gtggcggtgt
      241 cgaccgccct ggacaaggtc gctgccttcg ggattgaccc ggccaacgcg ttcggcttct
      301 gggactgggt cggcggtcgt tactccgtcg actccgcgat cggcacggct gtcgcggtgg
      361 ctatcgggcc ggagcggttc gccgagttgc tcgccggctt ccatgcggtt gacgagcact
      421 tccgcaccgc gcccctggag cgcaacgtgc ctgccctcat gggcctgctc aacgtctggt
      481 acgtcaacca cctcgacgcc cgcagccacg cggtgctccc ctacgcccag caactgcacc
      541 ggttcgcggc atacctgcag cagttgacga tggagtccaa cgggaagtcg gtccgttggg
      601 acggcacccc ggtgaccacc accaccggag aagtcttctg gggcgagccc ggcaccaacg
      661 ggcagcacgc gttctaccaa ctgctgcacc agggcaccca gctcatcccg gcggacttca
      721 tcgccgtggc cacaccggca taccccttga cggacggtgc cgccgacgtg cacgagctgt
      781 tcctcgcgaa cttcttcgcg cagaccgcgg cgctggcctt cggcaagacc gccgacgagg
      841 tgcgcgcgga ggggacggcc gaggcgatcg tgcccgcgcg ggtcttctcg ggcaaccgcc
      901 cgacgacctc gatcatggct cccgcgctga cgccatcggt ggtcggacag ctcgtcgcgc
      961 tctatgagca catcaccttc acgcagggca tcgtctgggg catcgactcc ttcgaccagt
     1021 ggggtgtcga gctcggcaag caactggcca agaagatcga gcctgcggtg gctggcgacg
     1081 aggtcgcgct cggcgaccag gacgcctcca cccaatcgct catccgctac tacctggctc
     1141 accgggagcg ctgaacgatc gatcagcccg gcagcggagt cagccctcgg acggctccgg
     1201 tgccgggctg tgtcgtgcgg cgaaggcgag ccactcgctg aggtggacgt agaccgcagg
     1261 gtggttgagc aggtcgaggt ggcgggcgcc gcccacaaca cggatgtccc tcgggtcgac
     1321 ctcgccccgg ctagccgcga cctgcgcgtc ggcgggacga accaacaggt cgcccaccca
     1381 acccgccacg agcccatcgc gtttcgacgg caccgtgccg ataacgacgc agtggcgtat
     1441 gccgcccgcc agcggcaccg ttcgcgccgg cgcggtgagc gcgccggagt cgacgtcgtg
     1501 ccagtcgtcg gcgagcaggt tcccgtgccc gaggtcaccc atccccgccg ggcgggacga
     1561 gatcacctcg gccagccatc gggtctccgg gagtctggca aggactgggg tgagccgggc
     1621 gacgccacgg acgatggcgg ccccctggtg cggagttccg atggtgaccg tgacccggac
     1681 cagactcggc cactggcggt cggacgaggc ctgggcgagg gcgctgtgca ggaccaaccc
     1741 gcccatcgag tggccgacga ggaccagccc gcacacgggg accggccatc ccgcgacgag
     1801 cgcgtcgagc aggtcatcga ggtgctggcc gttgttcgac acccgcagcc cggtgttgta
     1861 gcgcacccgc accggcgtcc acgcgccctc ggcccgcagt cgacgcgagt agctctcgcc
     1921 cggatgcccc cagttgcgtt cggagcggat gtcccaccag tcgtcggtct cgacgagccc
     1981 gtggatgaag accacaacga gcccggtcgc gtgcgggtat gccgcgtgta gacctccgcg
     2041 cgagaggggc acgtcgcgcc cctcggctcg cagggccatc ggcaggctga gcggcgagtg
     2101 tcgttgctga agtcggtcgc cgtagaggcc gttgagcacg gacaccgcct gataggcgcc
     2161 gggctcctcg acgaccggcc gaccgtcggc gaaacgtgcc ccgacccggg ccacaccatg
     2221 tccgatggcg cccagaccgc gtcccacggc ctgcccggcc caggtggtcg cctggcgatg
     2281 cgcggtgcgg acccgctcga tccggtcgcg gtgggccggc ccggcgatgc gcgccgcggc
     2341 gccgtagcca cgatcgagga tcgccgcgga cagttggccg ctgcgtctcg cggtgccgac
     2401 aagggtctcc cccaggaccc gaccgacctc tgcagcctcc gacgccttca ttactcaaga
     2461 gtaacttgct ggacgggtcg agccgaccgg cttcgggagg cgagcgggca ggcgggcagg
     2521 caggcgacgc ccgaggtatg ccggaccgcc gggagcagcg gtcacctcaa ggagcgccgc
     2581 gcaccgggga gagatacccc ggtcagccgt cgacggcgag gaccagcggg aggacagcgg
     2641 gagctccggc ggcccgcagg gctcgcgcgg cgacggtgag ggaccagcgc gagctcacca
     2701 ggtcgtcgac
//
LOCUS       DAOMZY010000409         2683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_38197_length_2683_cov_6.059361, whole genome shotgun sequence.
ACCESSION   DAOMZY010000409 DAOMZY010000000
VERSION     DAOMZY010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38197_length_2683_cov_6.059361"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /gene="lexA"
                     /locus_tag="PLK64_16195"
     CDS             <1..566
                     /gene="lexA"
                     /locus_tag="PLK64_16195"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HPV81336.1"
                     /translation="ALERKGYLRKDPNRPRALEVVSPDAKASDRGYRGSSGMPPSSAD
                     AWEHGEAHDTETDSGDARPEASYVPVVGRIAAGGPILAEEAIEDVFPLPRQIVGEGQL
                     FLLKVVGDSMVDAAICDGDWVVVRQQPTADNGDIVAAMIDGEATVKTFQRKAGRVLLI
                     PHNAAYEPIDGRDASILGKVTAVLRRI"
     gene            604..1413
                     /locus_tag="PLK64_16200"
     CDS             604..1413
                     /locus_tag="PLK64_16200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HPV81337.1"
                     /translation="MTTRLPFASYLDHLRIESEAFAVALTDVPDDRQVPTCPDWTALD
                     LAHHLTEVQSFWAAVVSGPLLTDEAAEDVAKPAPAASVPAQLKDLRAASACLAAALAT
                     TPADTPAWTWSTEQTVGFTFRRQAHEALVHRLDAQLTAGLRTPVDPTLATDGVDEALR
                     VMFGGCPPWGQITPVAGPTVRLVTSDTGASWLISPARFTGTDPDGTAYDDPDIVVAES
                     DSGGDVAATITGTAEDLLCWLWKRPTVGTLSQTGDESALAAVSQVLAQPIN"
     gene            complement(1406..2296)
                     /locus_tag="PLK64_16205"
     CDS             complement(1406..2296)
                     /locus_tag="PLK64_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HPV81338.1"
                     /translation="MSYAAPATQASHDPDLLAGLVSQVGAGGVFVLTGAGLSTDSGIP
                     DYRGRDGVRRVMPMQFGEFVGSSAARQRYWARSYVGWQRFRAAAPNDGHRSLARLGDL
                     GLFEALVTQNVDGLHQSAGSADVVELHGSLAHVVCLTCTDRSARHEVQERLAVANPGF
                     VDHARHAAADGSQVSSQIRPDGDIVLADEVVEAFHPPTCLVCGADTLKPDVVFFGESV
                     PRERVQRCFDALDASRSVLVLGSSLAVMSGYRFVRRAAARGIPVAIVTHGVTRGDAQA
                     TLRLDEPLAPTLGRLVAALS"
     gene            2447..>2683
                     /locus_tag="PLK64_16210"
     CDS             2447..>2683
                     /locus_tag="PLK64_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPV81339.1"
                     /translation="MIDAAGLRVMKAIADEGSFTAAATSLGFTQPAISQMVRRLEQRT
                     GTALVERLGRNVRLTEAGEVLARHAVTVLAALDAA"
BASE COUNT          434 a         1005 c          872 g          372 t
ORIGIN      
        1 ccgccctgga acgcaagggc tacctccgca aagaccccaa ccgcccccgt gcgcttgagg
       61 tcgtctcccc cgacgccaag gccagcgacc gcggttaccg gggcagcagc ggtatgccgc
      121 ccagcagcgc cgacgcgtgg gagcacggcg aggctcacga caccgagacc gactccggcg
      181 acgcccgtcc cgaggcgtcc tacgtccccg tggtgggccg catcgccgcc ggcgggccga
      241 tccttgccga ggaggccatc gaagatgtct tccccctccc ccgacagatc gtcggcgagg
      301 ggcagttgtt cctgctcaag gtcgtcggcg actcgatggt cgacgcggcg atctgcgacg
      361 gcgactgggt cgtcgtccgg caacagccga cggcggacaa cggcgacatc gtcgcggcaa
      421 tgatcgacgg cgaagccacc gtaaagacct tccagcgcaa ggccggccgc gtcctgctca
      481 tcccgcacaa cgcggcatac gagccgatcg acggtcgcga tgcgagcatc ctcggcaagg
      541 tgaccgcggt cctgcgccgt atttgagtgt cggcgccagg aacgcccctg agatgctgag
      601 gcgatgacca cccggctgcc gtttgccagt tacctcgatc acctccggat cgaatccgag
      661 gccttcgccg tcgccctgac ggatgtcccc gatgaccgac aggtgccgac ctgccccgac
      721 tggacggcgc tggacctcgc ccatcacctc accgaggtcc agtccttctg ggccgccgtc
      781 gtgagcggtc cgctgttgac cgacgaggcc gccgaggacg tggccaaacc tgcccccgct
      841 gcctcggtgc cggcccaact caaggacctg cgagcggcga gcgcttgtct cgcggccgcc
      901 ctggccacca cccctgcgga cacgcccgcc tggacctggt ccacggagca gaccgtcggg
      961 ttcacgttcc gacgccaggc ccacgaggcc ctcgtccacc gcctcgacgc ccaactcacc
     1021 gccggcctgc gcactcctgt ggatccgacg ctcgcgaccg atggggtcga cgaggcgctg
     1081 cgggtgatgt tcggcggctg ccccccgtgg ggtcagatca cgcccgtcgc cggccccacg
     1141 gtccggctgg tcacgagcga cacaggggcc tcgtggctga tctcgccggc ccgcttcacc
     1201 ggcaccgacc ctgacggcac cgcctatgac gaccccgaca tcgttgtggc agagtcggat
     1261 tcagggggcg atgtcgcggc aacgatcacc gggaccgccg aagacctgct gtgctggctc
     1321 tggaagcgcc ccaccgtcgg gacgctctcc cagacgggcg acgagtcggc cctggctgcg
     1381 gtcagccagg tgctcgccca acccatcaac tgagcgccgc gaccaaccgg ccgagcgtgg
     1441 gagcgagcgg ttcgtccagc cgcagggtcg cctgcgcatc tcctcgagtc actccgtggg
     1501 tgacgatggc cacgggtatg ccgcgtgccg ccgcccgccg cacgaatcgg tagcccgaca
     1561 tcaccgccaa ggacgagccg aggacgagaa ccgaccggct ggcgtccagc gcgtcgaagc
     1621 agcgctggac ccgctcgcgc ggcaccgact ccccgaagaa caccacgtcg ggcttgaggg
     1681 tgtcggcgcc gcacaccaga caggtcggcg ggtggaatgc ctcgacgacc tcgtcggcca
     1741 ggacgatgtc tccgtcgggc cggatctggc tgctcacctg ggagccgtcc gcagcagcgt
     1801 gccgcgcatg atcgacgaac ccggggttgg ccacggccag ccgctcttgg acctcgtgcc
     1861 gagcactgcg atcggtgcac gtgaggcaga cgacgtgtgc aagcgatcca tgcaactcga
     1921 cgacgtcggc cgagccggca ctctggtgca gcccgtcgac gttctgggtg accagagcct
     1981 caaacagacc gaggtcgccc agccgcgcga gtgaccgatg cccatcgttg ggcgcagccg
     2041 cccggaagcg ttgccagccg acataggacc tcgcccaata cctctggcgg gcagcgctgg
     2101 acccaacgaa ctcaccgaac tgcatcggca tcacccgacg gaccccgtcc ctccctcggt
     2161 agtcggggat cccggagtcg gtcgacaacc ctgccccagt gaggacgaac acgcccccgg
     2221 cgccgacctg cgacaccaag ccggccagca agtccgggtc gtggctcgcc tgcgtggcgg
     2281 gcgcggcata cgacatgccc gcagtatgcc gccctcccgg actcgcgcgc cgaccacggc
     2341 gaccgagcgg aaccggcgcg cccgaccccc accggggatg cgacgactgt cattacctac
     2401 cgggtggttc tgtaccttcg tgataactcg cggttatgat tgcgccatga tcgatgccgc
     2461 cggccttcgg gtgatgaagg ccattgccga cgagggcagc ttcaccgctg cggccacgtc
     2521 gctggggttc acccagcccg ccatctcgca gatggtccgc cgtctggaac agcgcacggg
     2581 caccgcgctc gtggagcgac tcggacgcaa cgtgcgcctc accgaagcgg gcgaagtcct
     2641 ggcccggcac gccgtcaccg tcctggcggc cctcgatgcc gca
//
LOCUS       DAOMZY010000410         2681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_38243_length_2681_cov_4.378522, whole genome shotgun sequence.
ACCESSION   DAOMZY010000410 DAOMZY010000000
VERSION     DAOMZY010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38243_length_2681_cov_4.378522"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PLK64_16215"
     CDS             <1..286
                     /locus_tag="PLK64_16215"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPV81340.1"
                     /translation="GIPPQSRAASRVFPRFLERYLDERAVSISDAVVTAARGLDVPPS
                     QVALAWSRDTPGVSAVLVGARTLTQLQTALGSESIDLPGPVRDALDDVSA"
     gene            complement(293..676)
                     /locus_tag="PLK64_16220"
     CDS             complement(293..676)
                     /locus_tag="PLK64_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81341.1"
                     /translation="MTADPRAALALLVSALERHLEVATTRRDPDDPGVIAAAEALAEA
                     FDDYDEVLFAATEVATPLAVYGDEDDFDDDDDEVVDPRAAVEGKVTVYAGLDDEDVDF
                     DDTDDEDDDDDEEDDEDDDEEDERG"
     gene            complement(747..2096)
                     /locus_tag="PLK64_16225"
     CDS             complement(747..2096)
                     /locus_tag="PLK64_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPV81342.1"
                     /translation="MTTSQANGIRPEDEVARLCAELIRIPSVNAGDNQGPGERAAAEW
                     VMEQLHEVGLEPALVESSPGRASVMVRIEGADPSLPGLVVHGHTDVVPANAADWQVDP
                     FGAEERDGCIWGRGAVDMKDMDAMILASVRDLARTGAKPARTTTFVFFADEEAGGVYG
                     SQWVVANHPEWFEGASEAISEVGGFSVTVPTRHTGAPTRAYLLQTAEKGIAWLRLHAH
                     GRAGHGSVPNDANAVVRLAQALARIDAHVWPREYVASVSGLLDGLSEITGIPYAAHDP
                     SVFPTDLLQTLGGAAGFVLGTLRDTAQPTMLSSGYKMNVIPQTATAALDCRFLPGHEE
                     SLMATIRELAGEHVEVEVIHHDRALDAPFTGSLVQAMKQALLAEDPGAHVLPYCLSGG
                     TDNKALARLGITGYGFAPLRLPPDLDFVSMFHGIDERVPVDSLQFGARVLRRFLDLA"
     gene            2214..>2681
                     /locus_tag="PLK64_16230"
     CDS             2214..>2681
                     /locus_tag="PLK64_16230"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPV81343.1"
                     /translation="MDESTIEVTSRDGIQLQVYRWAPDGEPRAVVQIQHGLGEHALRY
                     KRFARALTEVGYLVYAPDGRGSGRTAQGRFGHWGEDSWPGWVDDVARLNARIRTDHPG
                     LPLALFGHSMGCQVALEAFRLRREDVLGMVLICGSYGRVTHTFKGTDVMAQLLP"
BASE COUNT          357 a          868 c         1008 g          448 t
ORIGIN      
        1 cggcataccg ccgcagtctc gggccgcatc ccgggtcttc ccacggttcc tggagcgcta
       61 cctcgacgag cgggcggtct cgatctccga tgcggtcgtc acggccgcac gtgggttgga
      121 cgtcccgccg tcgcaggtcg cgcttgcttg gtctcgcgac acgccggggg tgagcgccgt
      181 gctcgtcggt gctcggacgc tgactcagtt gcagacggcg ctgggcagtg agtcgatcga
      241 ccttccgggc ccggtccgcg acgctttgga cgacgtttcg gcctgagccg gctcagccgc
      301 gctcgtcctc ttcgtcgtcg tcctcgtcgt cttcctcgtc gtcgtcgtcg tcttcatcgt
      361 cggtgtcgtc gaagtcgacg tcctcgtcgt cgaggccggc atagacggtg actttgccct
      421 cgaccgcggc gcgcgggtcg accacctcgt catcgtcgtc gtcgaagtcg tcctcgtcgc
      481 cataaaccgc cagcggcgta gcgacctccg tggccgcgaa tagcacctcg tcgtagtcgt
      541 cgaaggcttc cgccagggcc tcggccgcgg cgatgacgcc cgggtcgtcg gggtcgcgac
      601 gggtcgtggc aacctcaagg tggcgctcca gcgcagaaac gagcagggcg agtgcggcac
      661 gcgggtcggc ggtcatgcta cgaaggtatc gggtccacgt cacgaatggg ccaccggtcc
      721 ggcgaacgcc ctgtggacgg tgcctgtcag gccaggtcga ggaaccgccg caacaccctc
      781 gcgccgaact gcagggagtc gactggcacc cgctcgtcga tgccgtggaa catcgacacg
      841 aagtcgaggt ccggaggcag tcgcagcggc gcgaagccgt atccggtgat cccgagccgg
      901 gccagcgcct tgttgtcggt gccgccggag aggcagtagg gcaggacgtg ggcgccgggg
      961 tcttcggcca gcaaggcctg cttcatcgcc tgcaccagtg agccggtgaa gggcgcatcg
     1021 agcgcacgat cgtgatggat gacctcgacc tcgacgtgct cccccgcgag ctcgcggatc
     1081 gtcgccatga gcgactcctc gtgcccaggc agaaaccggc agtcgagggc ggcggtcgcc
     1141 gtctgcggga tgacgttcat cttgtatccg ctggagagca tcgtcggctg ggcggtgtcg
     1201 cgcagcgtcc cgagcacgaa tccggctgcg cccccgaggg tttggagcag gtcggtgggg
     1261 aagacggagg ggtcgtgcgc ggcatacggg atgccggtga tctccgagag gccgtcgagg
     1321 aggccgctca cggaggcgac gtattcgcgg ggccagacgt gcgcgtcgat gcgcgcgagc
     1381 gcctgcgcga ggcggacgac cgcgttggcg tcgttgggga ccgagccgtg gccggcgcgg
     1441 ccgtgggcgt gcaggcgcag ccaggcgatg cccttctcgg ccgtctgcag gaggtaggcg
     1501 cgggtgggtg ctccggtatg ccgcgtcggc accgtcacgc tgaagccgcc gacctcgctg
     1561 attgcctcgc tggccccctc gaaccactcg gggtggttgg cgacgaccca ctggctcccg
     1621 taaacgccgc cggcctcctc atcggcgaag aagacgaagg tcgtcgtgcg ggctggcttg
     1681 gcgccggtgc gggccaggtc gcggaccgac gcgagaatca tcgcgtccat gtccttcatg
     1741 tcgacggcgc cgcgacccca gatgcacccg tcgcgctcct cggcgccgaa cggatcgacc
     1801 tgccagtcgg ccgcgttggc ggggacgaca tcggtgtgac cgtgcacgac gaggccgggc
     1861 aggctcgggt cggcgccctc gatgcggacc atgacgctcg cacggccggg gctgctctcg
     1921 acgagggcgg gctccaggcc cacctcgtgg agttgctcca tgacccactc ggcggctgcc
     1981 cgctcccccg gcccctggtt gtcaccggcg ttgaccgacg ggatgcggat gagctcggca
     2041 cacagccggg ccacctcgtc ctcgggacgt atgccgttcg cctggctggt cgtcatggcc
     2101 ccaacctaac ctgggagggc tctgcaggcg gggtgggctt gtcgattcgg cgggtgtttg
     2161 tggggcgggt ttgcggtgga cgggtgcagg ggtgcgtgac aggctctggg ggcatggacg
     2221 agtcgacgat cgaggtcacc agccgggacg gcatacagct gcaggtctat cgctgggcgc
     2281 ccgatggtga gccgcgcgcg gtggtgcaga tccagcacgg tctgggtgag catgcgttgc
     2341 gctacaagcg atttgcgcgg gcgctgaccg aagttgggta tctcgtctat gcgccggacg
     2401 ggcgtgggtc ggggcggacg gcgcaggggc ggtttgggca ttggggcgag gactcgtggc
     2461 cggggtgggt cgacgatgtg gcgcggctca acgcgcggat ccggaccgat cacccggggc
     2521 tgccgctggc gctgttcggg cactcgatgg ggtgccaggt cgcgctggag gcgttccgcc
     2581 tgcggcgcga ggacgtgctc gggatggtgc tgatctgcgg ctcgtacggc cgcgtcacgc
     2641 acaccttcaa gggcaccgac gtgatggcgc agctcctccc g
//
LOCUS       DAOMZY010000411         2680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_38264_length_2680_cov_5.401905, whole genome shotgun sequence.
ACCESSION   DAOMZY010000411 DAOMZY010000000
VERSION     DAOMZY010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38264_length_2680_cov_5.401905"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PLK64_16235"
     CDS             <1..655
                     /locus_tag="PLK64_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPV81344.1"
                     /translation="NNPTGSALDLEIVRAVLDAAPDAVVVVDEAYAEFARPGTRSALS
                     LLAAYPQLVVTRTMSKAFALAGGRLGYLVAAPEVVDALRLVRMPYHLSTPTQEIARAA
                     LAHRLSLLAEVEAIKNQRDRIVETLAAQGFPPVPSDANFVLFGGLADAGRTWEALLAR
                     GVLVRDVGIAHHLRVTAGTPAETTAFLDAMAALPAEHRSQTAPELSAEPSASQGAPA"
     gene            652..1269
                     /gene="hisB"
                     /locus_tag="PLK64_16240"
     CDS             652..1269
                     /gene="hisB"
                     /locus_tag="PLK64_16240"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HPV81345.1"
                     /translation="MSDQPARRATVRRATSESSVELTVDLDGTGRSTVSTGVGFYDHM
                     LASLAKHALLDLDVAAVGDVDIDAHHTVEDVAICLGDALREALGDKRGISRFGDATVP
                     LDEALVQAVVDVAGRPYCVHSGEPAGQEFVVIGGSYIGSLTRHVFETLAHHAGIALHV
                     RVLAGRDPHHIVEAQFKAVARALRAAVAPDPRVHGIPSAKGTLSR"
     gene            1266..1931
                     /locus_tag="PLK64_16245"
     CDS             1266..1931
                     /locus_tag="PLK64_16245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81346.1"
                     /translation="MSPDAGLGDGAASAARSSGGGLLLVHAGVSTVREWALDGLGPVV
                     VVPDPSGWAILVPDGDRTFAAPPFDDGVGALLGRPVRRRLLPTIGVALVGTRLAISVV
                     PAVLRPGRSWLVWEPGAGLVRPAGLTPASVAQLAAAADRREHRDLVAAMLADGRGSAA
                     RVLRDVFEALSLPGVDFATGERVPVDEPGAVRIEPSREDVARFDRTVHEVRRWREEVE
                     GSP"
     gene            1928..2566
                     /gene="hisH"
                     /locus_tag="PLK64_16250"
     CDS             1928..2566
                     /gene="hisH"
                     /locus_tag="PLK64_16250"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HPV81347.1"
                     /translation="MSPTVVVFDYGSGNVRSAVRSLERVGAEVTLTAHADEALAADGL
                     VVPGVGNFHACMAGLRAADGARIIEVRLAGNRPVLGVCVGMQVLFDGSTEPAAHEEPG
                     LGEWPGMVERLVAPVVPHMGWSPVQVPDGSRLFAGVESERFYFVHSYAVQSWTLEPHG
                     PFAPRAPLVTWSEHGAPFVAAVENGPLAATQFHPEKSGDAGAHLLENWVRSL"
BASE COUNT          350 a          957 c          952 g          421 t
ORIGIN      
        1 caacaacccc accggctccg cgctcgacct ggagatcgtc cgggccgtgc tcgacgcggc
       61 gcccgacgcg gtggttgtcg tcgacgaggc gtacgccgag ttcgccaggc ccggcacacg
      121 gtcggcactc agcctgctgg cggcataccc gcaactcgtg gtgacccgga cgatgagcaa
      181 ggctttcgcg ctggccgggg ggcgattggg ctatctcgtg gccgcacccg aggtggtcga
      241 cgcgttgcgc ctcgtgcgga tgccctacca cctctccacc ccgacgcagg agatcgcccg
      301 ggccgccctg gcgcaccgct tgagcctgct ggcggaggtc gaggccatca agaaccagcg
      361 cgatcgcatc gtggaaaccc ttgcagcaca aggcttcccg cccgtgccta gcgatgccaa
      421 cttcgtcctg ttcggtgggt tggccgacgc tggccgcacc tgggaggcgt tgctcgcccg
      481 cggtgtgctc gtccgcgatg tcgggatcgc ccaccacctt cgggtgaccg ctggcacgcc
      541 cgcggagacg accgcgttcc tcgacgcgat ggccgccctg cctgccgagc accggagcca
      601 gaccgccccc gaactctctg ccgagccgtc cgccagccaa ggagcccctg catgagcgac
      661 cagcccgccc gccgcgccac cgtgcgtcgg gccacgagcg agtcgagtgt cgagctcacc
      721 gtcgatctcg acggcaccgg ccgcagcacc gtgtcgaccg gggtcgggtt ctacgaccac
      781 atgctcgcct ccctggccaa gcacgccctg cttgacctcg atgtcgcggc ggtcggggat
      841 gtcgacatcg acgcccacca caccgtcgag gacgtcgcca tctgcctcgg tgatgcgctc
      901 cgtgaggcgc tgggggacaa gcgcgggatc agccgcttcg gtgacgcgac ggtgccgctg
      961 gacgaggcgt tggtccaggc cgtcgtcgat gtcgccggac gcccctactg cgtccactca
     1021 ggggagccgg ccgggcagga gttcgtcgtc atcggcgggt cctacatcgg gtcgttgacc
     1081 cgccacgtct tcgagacact ggcccaccac gccggcattg ccctgcatgt gcgagtgctc
     1141 gccggacgcg atcctcacca catcgtcgag gcccagttca aggcagtcgc tagggccctg
     1201 cgggctgccg tcgcccccga tccgcgggtg cacggcatac ccagtgccaa ggggacgctg
     1261 tcgcggtgag ccccgatgcg ggcctcggtg acggcgcggc ctcggcagcc cggtcgagcg
     1321 gcggcggtct gctgctcgtg cacgccgggg tgtcgacggt acgggagtgg gcgctggacg
     1381 ggctcggccc agtggtcgtc gtcccagacc cgagcgggtg ggcgatcctg gtcccggacg
     1441 gggaccgaac cttcgcggcg ccgccgttcg acgacggcgt gggtgccctg ctcggtcgac
     1501 ccgtgcggcg gcgactgcta ccgacgatcg gtgttgccct ggtcggcacc cgattggcga
     1561 tcagcgtcgt cccggcggtg ttgcggccgg gtcggtcctg gctggtctgg gagccggggg
     1621 cagggctggt ccgcccggcc ggtctcaccc cggcgagcgt cgcgcaactg gcggccgcgg
     1681 ccgaccgccg cgagcaccgc gacctcgtcg cagcgatgct tgccgacggc cgtggctcgg
     1741 ccgctcgggt cctgcgggat gtcttcgagg cgctcagcct tcccggggtc gacttcgcca
     1801 ccggcgagcg agtgccagtg gatgaaccgg gtgcggtgcg catcgagccg tcgcgagaag
     1861 atgtcgccag gttcgatcgc acggtgcacg aggttcggcg ctggcgcgag gaggtggaag
     1921 ggagcccatg agtcccacag tggtcgtttt cgactatggc agtggcaacg tccgctcggc
     1981 ggtccggtcc ctcgagcggg tcggtgccga ggtcaccctc acggcacacg ccgacgaggc
     2041 gctggccgcc gatggtctgg tcgtcccggg ggtgggcaac ttccatgcct gcatggctgg
     2101 gctgcgtgct gctgacggtg ctcgaatcat cgaggtacgg ctggccggca atcggccggt
     2161 gctgggcgtc tgcgtcggga tgcaggtgct cttcgacggc tccaccgagc ccgccgccca
     2221 cgaggagccc ggtttggggg agtggcccgg catggtcgag cggctcgtcg ctccggtcgt
     2281 cccccacatg ggatggtcgc ccgtgcaggt ccccgacggg tcgcggctgt tcgccggcgt
     2341 cgagtccgag cgcttctact tcgtccactc gtatgccgtg cagtcctgga cgctcgaacc
     2401 gcacggaccc ttcgcccccc gagcgccatt ggtcacgtgg tccgagcacg gagcgccgtt
     2461 cgtcgccgct gtcgagaacg gtccgctggc ggccacgcag ttccaccccg agaagtccgg
     2521 cgatgccggc gcacacctgc tggagaactg ggtgaggtcc ctatgaacgc caaacgcgcc
     2581 tccaacaccc gccggacgac caccaccaga cgacgcaggt cgacgacgac gtcctcgggg
     2641 tatcggcgtc gtcgccgccc cggcctgtcg tcgacggtcg
//
LOCUS       DAOMZY010000412         2674 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_38434_length_2674_cov_3.724322, whole genome shotgun sequence.
ACCESSION   DAOMZY010000412 DAOMZY010000000
VERSION     DAOMZY010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38434_length_2674_cov_3.724322"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PLK64_16255"
     CDS             complement(<1..600)
                     /locus_tag="PLK64_16255"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HPV81348.1"
                     /translation="MAPRPIVIDCDPGHDDAVALLLAVGSPEVDLLAVTTTFGNCAVE
                     DATRNALQILTLAGATGVPVASGAAGGIDGKSTLGNYVHGTSGLDGPPMPAPASEVVP
                     AGAVELLRRTIQASAEPVTVVATGPITNVARLLLEHPEVKDGIAEVIFMGGSTERGNH
                     TPTAEFNTYADPEALDVVLRSGLVVRMVGLNLTHQALATP"
     gene            complement(600..1352)
                     /locus_tag="PLK64_16260"
     CDS             complement(600..1352)
                     /locus_tag="PLK64_16260"
                     /inference="COORDINATES: protein motif:HMM:NF018757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HPV81349.1"
                     /translation="MRVLLAGESWISNSTHYKGFDSFSSTTFETGADHFIAAAASQGI
                     TVEQLYAHDVPGRFPRTVEALSAYDVVILSDIGYNSFVLPNEVWLKGQRTDNPLVSLV
                     EWTRAGGGLMMAGGYLSFQGFQARANFARSPIAEALPVDMLTSDDRAEKPEGATCRLV
                     SADHEIARAWTGDAPQLLGYNVVTAKPDAEVVATVDGDVLIATRSFGQGRSLVWTSDI
                     GPHWCPTPFLEWDGFGPLVGAMLRWLANGRAS"
     gene            complement(1355..2398)
                     /locus_tag="PLK64_16265"
     CDS             complement(1355..2398)
                     /locus_tag="PLK64_16265"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81350.1"
                     /translation="MTSVMTVTGPVDSADLGTTLTHEHIFNDVTSWWHETASTGWSAE
                     DFASRPVTMDLLWDLRHDPFGNRDNCRLDSEPLAVTEIGRYAALGGRTIIDATGLGVG
                     RDLALLRRVSAATGVNIVAGTGYYLDGALPAEVASFGPEQFAEAIVRDVVDGEDGIRP
                     GIIGEIGVSADFTPGERASLEGALIAQRELHLPIEVHLPAWFRRAGEVLDLAEHHGVD
                     PSMVVLCHMGPSGDDLAYQEQLLRRGAWVQYDMIGMEVFYADQGVQCPSDEQNATWLC
                     RLAERGHLGRLLVSQDIFIKSLLRHYGGPGYGHILQYFVPRLRRLGMTEDDVTQLLTT
                     NPRTLFETAHKEN"
     gene            complement(2395..>2674)
                     /locus_tag="PLK64_16270"
     CDS             complement(2395..>2674)
                     /locus_tag="PLK64_16270"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HPV81351.1"
                     /translation="VATRLASGSSNSGTAFELEVITAVVLGGTSLMGGRGSIAGSAIG
                     AITLGIISNGLVLLRVDVYWVPIVQGVILIVAIFLNSKLFARLVRSKT"
BASE COUNT          431 a          897 c          938 g          408 t
ORIGIN      
        1 gggggtcgcc aacgcctggt gggtcaggtt gaggccgacc atgcggacca cgagcccgct
       61 gcgcaggacg acgtccaaag cctccgggtc ggcgtaggtg ttgaactcag ccgtcggggt
      121 gtggttgccc cgctcggtcg acccacccat gaagatgacc tcggcgatgc cgtccttcac
      181 ctcagggtgc tccagaagca gccgggccac gttggtgatg ggcccggtgg cgacgacggt
      241 gaccggctcc gcgctggcct ggatggtgcg gcgcaacaac tcgaccgccc ctgcagggac
      301 cacctcgctc gccggagccg gcatcggcgg cccgtccagg ccgctcgtgc cgtggacgta
      361 gttgcccagc gtggacttcc cgtcgatccc tccggctgcg ccactggcca ccgggacacc
      421 ggtggcgccc gcgagcgtga ggatctgcag ggcgttgcgg gtcgcgtcct ccacggcgca
      481 gttcccgaac gtggtcgtca ccgccagaag gtccacctcg ggggacccga cagcgaggag
      541 cagcgcgacc gcatcgtcgt ggcccgggtc gcagtcgatg acgatcggcc gtggtgccat
      601 caggacgccc gtccgttggc gagccaacgc aacatggcgc cgacgagggg accgaacccg
      661 tcccactcga ggaacggtgt cgggcaccag tgcgggccga tgtcggaggt ccagacgaga
      721 gatcggccct ggccgaacga ccgggtggcg atgaggacgt caccgtcgac ggtggccacg
      781 acctcggcat ccggcttggc cgtgacgacg ttgtagccca gcagttgcgg cgcatcgccg
      841 gtccaggccc gcgcgatctc gtggtccgcg gacaccagcc ggcaggtcgc gccctccggc
      901 ttctcggccc ggtcgtcgct ggtgagcatg tcgaccggaa gcgcctccgc gatcgggctg
      961 cgggcgaagt tggcccgagc ctggaacccc tggaaactga gatacccgcc ggccatcatc
     1021 agaccgccgc cggccctcgt ccattcgacg agcgacacga gcgggttgtc ggtccgctgc
     1081 cccttgagcc agacctcgtt gggcaggacg aaggagttgt agccgatgtc cgagaggatg
     1141 acgacgtcat aggccgacag cgcctcgacg gtgcggggga accggcccgg cacgtcgtgc
     1201 gcatagagct gctcgacggt gatgccctgc gaggccgcag ccgcaatgaa gtggtccgca
     1261 cccgtctcga aggtcgtcga cgagaagctg tcgaagccct tgtagtgcgt ggagttcgag
     1321 atccagctct cgcccgcgag caacacacgc atggtcagtt ctccttgtga gcggtttcga
     1381 agagggtccg tgggttcgtc gtcagcagct gcgtgacgtc gtcctcggtc atgccgagtc
     1441 gacgcagtcg cgggacgaag tactgcagga tgtgcccgta gccgggcccg ccgtaatggc
     1501 gcagcagcga cttgatgaag atgtcctggg agacgagcag ccttccgagg tggccgcgct
     1561 cggcgaggcg acacagccag gtggcgttct gctcgtcgga tgggcactgc acgccctggt
     1621 cggcgtagaa gacctccatg ccgatcatgt cgtactgcac ccaggccccc cgccggagga
     1681 gttgttcctg ataggcgagg tcgtcgccgg agggccccat atggcagagc accaccatgg
     1741 acgggtcgac gccatggtgt tcggccaggt cgagcacctc gccggctcgt cggaaccagg
     1801 ccggcagatg gacctcgatc ggcaggtgca gctcccgctg cgcgatcagc gcgccctcga
     1861 ggctggcgcg ctccccaggc gtgaagtcgg cgctgacgcc gatctccccg atgatcccgg
     1921 gccggatgcc gtcctcgccg tcgacgacgt cgcgcacgat cgcctcggcg aactgctcag
     1981 ggccgaacga cgcgacctct gccggcaggg cgccgtcgag gtaatacccc gtccccgcaa
     2041 cgatgttgac gccggtcgcc gcactgacgc gacggagcag cgcgaggtct cgaccgacgc
     2101 ccagcccggt ggcgtcgatg atcgtgcgcc cgccgagggc ggcataccgg ccgatctcgg
     2161 tgaccgcgag cggttcgctg tcgagccgac aattgtcacg gttgccgaac gggtcgtgcc
     2221 gcaggtccca caacaggtcc atcgtcaccg gccgcgaggc gaaatcctca gccgaccacc
     2281 ccgtgctggc ggtctcgtgc caccacgacg tcacgtcgtt gaagatgtgc tcgtgggtca
     2341 gggtcgttcc gaggtcggcg gagtcgaccg gcccggtcac ggtcatgacg ctggtcatgt
     2401 cttggaccgg accaggcgag cgaacagctt gctgttcaag aagatggcga cgatgaggat
     2461 gacgccctgc acgatcggca cccaatagac gtcgacccgc aggagcacca ggccgttgct
     2521 gatgatgccc agggtgatcg cgccgatggc cgatccggcg atggagccgc ggccacccat
     2581 gaggctggtg ccgccgagca cgacggccgt gatgacctcg agttcgaagg ccgtgcccga
     2641 gttggacgac ccgctggcca gtcgggtcgc gacg
//
LOCUS       DAOMZY010000413         2673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_38452_length_2673_cov_6.652788, whole genome shotgun sequence.
ACCESSION   DAOMZY010000413 DAOMZY010000000
VERSION     DAOMZY010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38452_length_2673_cov_6.652788"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2199)
                     /gene="acnA"
                     /locus_tag="PLK64_16275"
     CDS             complement(<1..2199)
                     /gene="acnA"
                     /locus_tag="PLK64_16275"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_170844367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HPV81352.1"
                     /translation="MGNVAASVNSFGAKGQLEVGDASYTIFRLAAVDGSATLPYSLKV
                     LLENLLRTEDGANITAEHIRAIGNWDENADPDTEIQFTPARVVMQDFTGVPCVVDLAT
                     MREAVVALGGDPSKINPLAPAELVIDHSVQIDVFGNAGAFEKNVELEYTRNRERYQFL
                     RWGQTAFEEFKVVPPGTGIVHQVNIERLARTVMVRDGLAYPDTCVGTDSHTTMVNGLG
                     VLGWGVGGIEAEAAMLGQPVSMLIPRVVGFKLTGATTPGVTATDVVLTITDMLRKHGV
                     VGKFVEFYGEGVGTIPLANRATIANMSPEFGSTCAIFPIDDKTIDYLRLTGRADDAVA
                     LVEAYAKDQGLWLDPAAEPRFSEKLELDLSTVVPSIAGPKRPQDRIELSSAKAAFATS
                     LPTYTSDPGKRVPVQMADGRSFELFNGAVSIASITSCTNTSNPSVMIAAGLVARKAAA
                     KGLARKPWVKTSMAPGSKVVTGYYEAAGLWPDLEAIGFYLVGYGCATCIGNSGPLEPE
                     ISEAINANDLAVTAVLSGNRNFEGRISPDVKMNYLASPPLVIAYALAGTMDFDFETDS
                     LGTDQDGNPVYLRDIWPSPEEIQSLMDSSIKKELFTKEYADVFAGDSRWQSLQTPVGK
                     TFEWDGESTYVRKPPYFEGMALEPSPVADIAGARVLAFLGDSVTTDHISPAGSIKVDS
                     PAGKYLAAHGVERKDFNSYGSRRGNHEVMIRGTFANIRLKNLLLDGVEGGF"
     gene            2382..2624
                     /locus_tag="PLK64_16280"
     CDS             2382..2624
                     /locus_tag="PLK64_16280"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HPV81353.1"
                     /translation="MTVLPVNVDDDLIELLDAQAAESGRSRSEVLAAAIRRGLGGGRL
                     GQTLAAYRTGKEISEEDAMALAKDELAASRAERATA"
BASE COUNT          441 a          864 c          917 g          451 t
ORIGIN      
        1 gaagccaccc tcgaccccgt cgagcaggag gttcttgagc cggatgttgg cgaaggtgcc
       61 gcggatcatc acctcgtggt tgccgcgacg cgagccgtag gagttgaagt ccttgcgctc
      121 gacgccgtgg gccgcgaggt acttaccggc cgggctgtcg accttgatcg agccggccgg
      181 gctgatgtgg tcggtcgtca ccgagtcacc gaggaacgcc aggacgcgag cgccggcgat
      241 gtcggcgacg ggcgagggct ccagggccat gccctcgaag taggggggct tgcggacgta
      301 ggtcgactcg ccgtcccact cgaaggtctt gccgacgggg gtctgcagcg actgccaccg
      361 cgagtcgccg gcgaagacgt cggcatactc cttggtgaac agctccttct tgatggagga
      421 gtccatgagc gactggatct cctcgggcga gggccagatg tcgcgcaggt agaccgggtt
      481 gccgtcctgg tcggtgccga gcgagtcggt ctcgaagtcg aagtccatcg tccctgccaa
      541 ggcgtaggcg atgaccaacg gcggcgaggc gaggtagttc atcttgacgt cggggctgat
      601 ccgaccctcg aagttgcggt tgcccgacaa gactgcggtg acggccaggt cgttggcgtt
      661 gatcgcctcg gagatctccg gctccaaggg gccagagttg ccgatgcacg tcgcgcagcc
      721 gtaaccgacg aggtagaagc cgatggcctc gaggtccggc cacaggccgg cggcctcgta
      781 gtagccggtg acgaccttgg agccgggggc catcgaagtc ttcacccagg gcttgcgggc
      841 cagacccttc gcggccgcct tgcgagcgac cagacccgcg gcgatcatca ccgacgggtt
      901 cgacgtgttg gtgcacgagg tgatcgacgc gatggacacc gccccgttga acagctcgaa
      961 ggaacgcccg tcggccatct gcaccgggac ccgcttgccg gggtcgctcg tgtaggtcgg
     1021 cagcgacgtg gcgaaggccg ccttggccga ggacagctcg atgcggtcct gaggacgctt
     1081 cgggccggcg atggacggga cgaccgtgga caggtcaagc tcgagcttct cgctgaagcg
     1141 gggctcggcg gccgggtcga gccacaggcc ctggtccttg gcgtaggcct cgacgagggc
     1201 gacggcgtca tcggcccggc cggtgaggcg caagtagtcg atcgtcttgt cgtcgatggg
     1261 gaagatcgca caggtcgagc cgaactcggg gctcatgttg gcgatcgtcg cgcggttggc
     1321 cagcgggatc gtgccgacac cctcgccgta gaactcgacg aacttgccga ccacgccgtg
     1381 cttgcgcagc atgtcggtga tggtgaggac gacatccgtc gcggtgacgc caggggtcgt
     1441 cgcgccggtc agcttgaagc ccaccacccg cgggatgagc atcgacaccg gctgcccgag
     1501 catcgcggcc tcggcctcaa tgccgccgac gccccagccc aggacaccca gtccgttgac
     1561 catcgtcgtg tgcgagtcag tgcccacgca ggtgtcgggg taggccaagc cgtcgcggac
     1621 catgacggtg cgggccaggc gctcgatgtt gacctggtgg acgatgccgg tgcccggcgg
     1681 gacaaccttg aactcctcga acgccgtctg cccccagcgc aggaactggt agcgctcgcg
     1741 gttgcgggtg tactccagct cgacgttctt ctcgaaggcg cccgcgttgc cgaagacgtc
     1801 aatctgcacc gagtggtcga tgaccaactc ggccggagcg agcgggttga tcttggacgg
     1861 gtcgccgccg agcgcgacga cggcctcacg catggtcgcc aggtcgacaa cacacggcac
     1921 gccggtgaag tcctgcatga cgacgcgggc gggggtgaac tggatctctg tgtccgggtc
     1981 agcgttctcg tcccagttgc cgatggccct gatgtgctcg gcggtgatgt tcgcgccgtc
     2041 ctcggtccgc aggagattct ccaagagaac cttcaggctg tagggcagcg tcgcgctccc
     2101 atcgaccgca gcaaggcgga agatcgtgta ggacgcatca ccgacctcca gctgtccttt
     2161 ggccccgaag ctgttcaccg atgctgcgac gttgcccacg tcggctccct tcctggttca
     2221 catatcttga cgtcaagata aattagcacg tccacctacg atcctgcact acctgtccgc
     2281 ctgcagaaca gcaacaccct ccaagcgcag gctccgccct gtcggtaccg cccgcgtaga
     2341 ctaggcaccc acctgttgac accagcccta ggaggtgccc gatgacagtt cttccggtca
     2401 acgtcgacga cgacctgatc gagttgctcg acgcccaggc ggccgagtcg ggcaggtccc
     2461 gcagcgaagt actggctgct gccattcgcc gcgggctcgg tggcggtcgt ctcgggcaga
     2521 cgctggctgc ctaccggacc ggcaaggaaa tctcggagga ggacgcgatg gctctcgcga
     2581 aagacgagct cgccgcctcc cgggccgagc gcgccaccgc gtgagcggtg ctccaccgcg
     2641 tcgggtcgtc atcgacccca atgtgttcat ctc
//
LOCUS       DAOMZY010000414         2670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_38502_length_2670_cov_6.208795, whole genome shotgun sequence.
ACCESSION   DAOMZY010000414 DAOMZY010000000
VERSION     DAOMZY010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38502_length_2670_cov_6.208795"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /gene="ilvB"
                     /locus_tag="PLK64_16285"
     CDS             <1..1396
                     /gene="ilvB"
                     /locus_tag="PLK64_16285"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HPV81354.1"
                     /translation="RGITMPITKHNYLVTNPDEIPRAIAEAFHIAGTGRPGPVLVDIS
                     KDALTGSTTFSWPPVIDLPGYRPVARPHGKQIREAARYIAAARQPLFYIGGGVIRARA
                     SEVLRTLVDLTQIPVVTTLMARGAVPDSHPLHLGMPGMHGSVAAVTALQKTDLIIALG
                     TRFDDRVTGQLSTFAPNAKVIHADIDPAEISKNRTADVPIVGDCKDVITDLIEAITAE
                     QAAHGAAEYEAWQATVAEWKETYPVGYTTTPDGLSPQYVIERLGALSGPEATYVAGVG
                     QHQMWAAQFVQYSRPNSWINSGGLGTMGFAVPAAMGAKVAEPHRTVWAIDGDGCFQMT
                     NQELATCVINDIPIKVAVINNSSLGMVRQWQTLFYNERYSNTDLHTSHNQQRIPDFVK
                     LGDAYGCASWRVERPEEVDEAIIKALEINDRPVVIDFVVHRDAMVWPMVPAGVSNDKI
                     LIARGGAPLWEREA"
     gene            1457..1969
                     /gene="ilvN"
                     /locus_tag="PLK64_16290"
     CDS             1457..1969
                     /gene="ilvN"
                     /locus_tag="PLK64_16290"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HPV81355.1"
                     /translation="MPKNTLSVLVENKPGVLARIAALFSRRGFNIDSLAVGPTEHPDV
                     SRMTVVVDVDELPLEQVTKQLNKLVEVLKVVELEPQSSVQRQIVLVKVRADASNRSHI
                     VETAELFRAKVVDVASDSLTIEATGKVEKIQALLGVLEPFGIRELVQSGVVAVGRGPR
                     SITDRALRSA"
     gene            2094..>2670
                     /gene="ilvC"
                     /locus_tag="PLK64_16295"
     CDS             2094..>2670
                     /gene="ilvC"
                     /locus_tag="PLK64_16295"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HPV81356.1"
                     /translation="MADMIYDDDADLSIIQGMKVAVIGYGSQGHAHALNLRDSGVDVR
                     VGLAEGSKSRAKAEAEGLPVKSVAEATKEADLVVILTPDQVQRHVYSDEIKPNLKEGA
                     GLLFGHGFNIRFGYITPEPGSDVVMVAPKGPGHLVRREFNDGRGVPVLVCVEQDASGR
                     AWDIAKSYAKAIGGLRAGGIKTTFTEECETDL"
BASE COUNT          467 a          975 c          842 g          386 t
ORIGIN      
        1 ccgcggcatc acgatgccga tcaccaagca caactacctc gtgaccaacc ccgacgagat
       61 ccctcgggcg atcgccgagg ctttccacat cgccgggacg ggccgtccgg gtccggtcct
      121 cgtcgacatc agcaaggacg ccctcaccgg gtcgacgacg ttcagctggc cgccggtcat
      181 cgacctgccg ggctaccgtc cggtggcccg tccgcacggc aagcagatcc gtgaggcggc
      241 tcgctacatc gccgctgccc gtcagccgct gttctacatc ggcggcggcg tgatccgggc
      301 tcgcgcctcg gaggtcctgc ggaccctcgt cgacctcacc cagatcccgg tcgtcaccac
      361 gctcatggcc cgcggcgcgg tccccgacag ccaccctctg cacctgggta tgccgggtat
      421 gcacggctcc gtggccgccg tcaccgccct gcagaagacc gacctcatca tcgccctcgg
      481 cacgcggttc gacgaccggg tcaccggcca gttgtccacc ttcgcgccga acgccaaggt
      541 catccacgcc gacatcgacc cggccgagat ctccaagaac cgcaccgccg acgtgccgat
      601 cgtgggcgac tgcaaggacg tcatcaccga tctcatcgag gcgatcaccg ccgagcaggc
      661 cgcccacggc gccgccgagt acgaggcctg gcaggcgacg gtggccgagt ggaaggagac
      721 ctacccggtc ggctacacca cgaccccgga cggtctgtcg ccgcagtacg tcatcgagcg
      781 gctcggtgcg ctgtccgggc ccgaggcgac ctatgtcgcg ggtgtgggtc agcaccagat
      841 gtgggcagcg cagttcgtgc agtactcccg gccgaattcg tggatcaact ccgggggcct
      901 gggcaccatg ggcttcgccg ttccggcagc catgggcgcc aaggtcgccg agccgcaccg
      961 cacggtgtgg gccatcgacg gtgatgggtg cttccagatg accaaccagg agttggccac
     1021 ctgcgtcatc aacgacatcc ccatcaaggt cgcggtcatc aacaactcga gcctgggcat
     1081 ggtccggcag tggcagacgc tcttctacaa cgagcgctac tccaacaccg acctgcacac
     1141 gagccacaac cagcagcgca tcccggactt cgtcaaactc ggcgacgcct acggctgcgc
     1201 gagctggcgg gtcgagcggc ccgaagaggt cgacgaggcg atcatcaagg ccttggagat
     1261 caacgaccga cccgtcgtca tcgacttcgt cgtccaccgg gacgccatgg tgtggccaat
     1321 ggtgccggcc ggcgtgagca acgacaagat cctcatcgcc cgcgggggag cccccctgtg
     1381 ggagcgcgaa gcctgagccg tccgcagacc gcccccacca gcctccccag ccgcagcagc
     1441 acagagagac gccgaaatgc ctaagaacac cttgtccgtc ctcgtcgaga acaagcccgg
     1501 tgtgctcgcc cggatcgccg ccctgttctc ccgtcggggg ttcaacatcg acagcctggc
     1561 cgtcggcccg accgagcacc cggacgtgtc ccggatgacc gtcgtcgtcg acgtcgatga
     1621 gctgccgctg gagcaggtga ccaagcagct caacaaactc gtcgaggtcc tcaaggtcgt
     1681 cgagctcgag ccgcagtcgt cggtccagcg ccagatcgtc ctggtcaagg tgcgtgccga
     1741 cgcctcgaac cggtcgcaca tcgtcgagac cgctgagctc ttccgggcca aggtcgtcga
     1801 cgtggcgtcc gacagcctga cgatcgaggc gaccggcaag gtcgagaaga tccaagcgct
     1861 gctcggtgtc ctggagccct tcggcatccg tgaactcgtc cagtcgggcg tcgtcgcggt
     1921 cggccgcggg ccccgatcca tcaccgaccg agccctgcgt agcgcctgac gcagcctcac
     1981 tcgaggtgac cgcccgctgg ccgagcggcc cgcggggtga gcgcacggca taccctctag
     2041 tcccaactgc accacccacc gaccgcacga accagctaag gagtggccct ctgatggccg
     2101 acatgatcta cgacgacgac gccgacctga gcatcatcca agggatgaag gtggccgtca
     2161 tcggctacgg cagccagggc cacgcccacg cgctcaacct gcgcgactcc ggggtcgacg
     2221 tgcgcgtcgg cctcgccgag ggcagcaaga gccgggccaa ggccgaggcg gaggggctgc
     2281 cggtcaagtc ggtggccgag gcgaccaagg aggccgacct cgtcgtcatc ctcacccccg
     2341 accaggtgca gcgccacgtc tactccgacg agatcaagcc caacctcaag gagggcgcgg
     2401 gcctgctgtt cggacacggt ttcaacatcc ggttcggtta catcacgccc gagccgggct
     2461 ccgacgtcgt catggtcgcc cccaagggcc ccggccacct cgtgcgccgc gagttcaacg
     2521 acggccgtgg cgtcccggtc ctcgtctgcg tcgagcagga tgcctccggt cgggcctggg
     2581 acatcgccaa gtcctatgcc aaggcgatcg gtggcctgcg cgccggcggc atcaagacga
     2641 ccttcaccga ggagtgcgag accgacctgt
//
LOCUS       DAOMZY010000415         2638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_39208_length_2638_cov_6.511808, whole genome shotgun sequence.
ACCESSION   DAOMZY010000415 DAOMZY010000000
VERSION     DAOMZY010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39208_length_2638_cov_6.511808"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PLK64_16300"
     CDS             complement(<1..224)
                     /locus_tag="PLK64_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPV81357.1"
                     /translation="MEPRDQLTSGQAARILGCSRQHVVDLCDSGKLRCSVIGSHRRIA
                     RADLKSFLDTGRALRREEERSLWLHRALLGP"
     gene            373..693
                     /locus_tag="PLK64_16305"
     CDS             373..693
                     /locus_tag="PLK64_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017823063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPV81358.1"
                     /translation="MGTVTRQSESLRAAIACHRDALDAILARYAATNPRIFGSVARGD
                     ATPDSDIDLMVDLLPRRGNELLRVSGIAEELSEVLGTRVDVVAASLLRGEVSSTALAD
                     LVRV"
     gene            690..1010
                     /locus_tag="PLK64_16310"
     CDS             690..1010
                     /locus_tag="PLK64_16310"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HPV81359.1"
                     /translation="MSRSEEERFADIRNAIRRCNDYAPFLRSDDLASMAYDALLRNLA
                     VIGEAVRALPSETRESMPEVPWAAIAGLRNVVVHEYFRVNPDLILDIVDHALGELARS
                     IDNR"
     gene            complement(1080..2072)
                     /locus_tag="PLK64_16315"
     CDS             complement(1080..2072)
                     /locus_tag="PLK64_16315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HPV81360.1"
                     /translation="MKAFVVTHYGPDGLAAAEVATPTAGPGDVLVDIRAASINPLDKM
                     IRNGEFKHLIKYKPPFALGHDLSGVITAVGSDVRGFAVGDEIYARPRDGRIGAFAGTI
                     AIDAGDVARKPTSLSFEEAAAVPLVALAAWQALADVAGVQPGQKVLVHAGAGGLGSTV
                     IQVAKHLGAYVATTARTSDIDKVRSLGADEVIDFTTTDFADVLSGYDVVLDSLGLASL
                     QKSLTVLRPGGLAISVVGPPDPAFAAQLGMPFLKPVMAVMSRKVRGAAKKLGVRYSFL
                     FMKASGAQLATLAALYDNGTLRPVLDRTFPFDETVEAMAYVEQGKAKGKVVITR"
     gene            complement(2069..>2638)
                     /locus_tag="PLK64_16320"
     CDS             complement(2069..>2638)
                     /locus_tag="PLK64_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPV81361.1"
                     /translation="AGRRFKSDGIDGSGIATLVADAGLTNGAFYNHFASKDDLIATVV
                     AAQLTDQAAVIDALPVGRASMQTYLRQYLSPAHRDDRAGGCPSAALLDEIGRCDEATR
                     AAYTVGVEPMIAAVARHLDDGDPQGAQERAIGLLTLLVGSLQLARAVTNPALSQQILA
                     TAYTHALSLAGVQPGASTLPVAPPTQESS"
BASE COUNT          430 a          875 c          909 g          424 t
ORIGIN      
        1 ggccccagca gcgctcggtg cagccacagt gagcgctctt cttcccggcg cagcgcccgg
       61 ccggtgtcga ggaaggactt gaggtcggct cgggcgatcc gccgatgcga gccgatcact
      121 gagcagcgca gttttccgct gtcgcagagg tccacgacat gctggcggga acagcccaag
      181 atccgtgccg cctgcccgct ggtcagttgg tccctcggct ccatcgcctc agcatgtggg
      241 ctaaacacca aaaacacaac gtcgcatgcc gtttggctcg ggtggggagg tccggtcgaa
      301 ggcgcggccc gatatattgc cccgggctgt ttcggagcca gctgagaaca ctgcgccgtt
      361 acccttggct gcatgggcac agtcacgagg cagagcgagt cgctgcgagc ggcgatcgcg
      421 tgccatcgcg acgcgctcga tgcgatcttg gctcgctacg cggcaacgaa cccacggatc
      481 ttcggctccg ttgcgcgagg ggatgccacg cctgacagtg acatcgacct catggttgac
      541 ctgctacccc gtaggggcaa tgaactgctg agagtctccg ggattgcgga ggagttgagc
      601 gaggtgctcg gcacccgagt cgatgtggtc gcggcgtcgt tgctgcgcgg cgaggtgtcc
      661 tcgacggcgc tcgcggatct ggtgcgagtg tgagccggtc cgaggaggag cggttcgcgg
      721 acatccgtaa cgcgatcagg cgatgcaacg actacgcccc atttctgcgg tcggatgatc
      781 tcgcctccat ggcgtacgac gcactccttc gcaaccttgc cgtgatcggc gaggcagtgc
      841 gggcgctgcc gagcgaaacc cgggagtcga tgccagaggt gccctgggcg gccatcgccg
      901 gtctacgcaa cgtcgtcgtg cacgagtact tccgcgtgaa ccctgacctc atcctcgaca
      961 tcgtcgacca tgcgctcggc gaattggccc gatcgatcga caaccgctga cccactcagg
     1021 ccttcgtcac tacgtaggca gccagccgcc gggttgcgcc gacaaccgcc cgacaacact
     1081 caccgggtga tcacgacctt gcccttggcc ttgccctgct cgacgtaagc catcgcctcc
     1141 acggtctcgt cgaacgggaa ggtccggtcc agcaccggcc gcagcgtgcc gttgtcatag
     1201 agcgcagcca gtgtcgccaa ctgggcgccg ctcgccttca tgaacaggaa cgaatagcgg
     1261 accccgagct ttttcgctgc gccgcgcacc ttgcgactca tgaccgccat caccggcttg
     1321 aggaacggca tacccaattg ggctgcgaac gctgggtccg gcgggccgac gacgctgatc
     1381 gcgaggccac ctggccgcag caccgtcagc gatttctgca ggctcgcgag cccgagcgag
     1441 tcgagcacga cgtcataccc gctgagcaca tccgcgaagt cggtggtcgt gaagtcgatg
     1501 acctcgtcgg cgccgagcga acggaccttg tcgatgtcgc tcgtgcgcgc agtcgtggcg
     1561 acgtaggcac ccagatgctt ggccacctgg ataacggtcg acccgaggcc gccagccccg
     1621 gcgtggacaa ggaccttctg acccggctgc acgccggcca catcggcgag cgcttgccag
     1681 gcggccagcg ccacgagagg cacggcggcg gcctcctcga acgacagcga ggtgggcttg
     1741 cgcgccacgt cgcccgcgtc gatggcaatc gtcccggcga acgcgccgat ccggccgtcg
     1801 cgcggacgcg catagatctc gtcgccgacc gcgaagccgc gcacgtcgga gcccacggcg
     1861 gtgatcacgc cggagaggtc gtggccgagg gcgaacggcg gcttgtactt gatgaggtgc
     1921 ttgaactccc cgttgcggat catcttgtcc agcgggttga tgctcgccgc gcggatgtca
     1981 accagcacgt cgcccggccc ggcggtgggc gtggcgacct cggcggctgc cagcccgtcg
     2041 ggtccgtagt gggtgacgac aaaggccttc atgacgactc ctgggtgggg ggtgcgacgg
     2101 gcagtgtgga ggcgcccggc tggacgcccg cgagggacaa ggcatgggtg tatgccgtgg
     2161 caaggatctg ttgggacagc gccggattcg tgacggcgcg ggccaactgc agggagccga
     2221 cgagcagtgt gagcaagccg atcgcgcgct cctgcgcccc ttgcggatcg ccgtcgtcaa
     2281 ggtgccgcgc gacggccgcg atcatcggct cgacaccgac ggtgtatgcc gcgcgcgtcg
     2341 cctcgtcgca acggccgatc tcgtcgagca gggcggccga cgggcaaccg ccggcgcggt
     2401 cgtcgcggtg tgcgggggag aggtactgcc gcagataggt ctgcatcgag gcccggccta
     2461 ccggcaacgc gtcgatgacc gcggcctggt ccgtcaactg cgcggccacg acagtggcga
     2521 tcaggtcgtc cttggacgcg aagtggttgt agaaggcgcc gttcgtgagc cccgcgtcgg
     2581 caaccagcgt ggcgatgccg gacccgtcga tgccgtcgct cttgaaccga cgaccggc
//
LOCUS       DAOMZY010000416         2621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_39617_length_2621_cov_5.247077, whole genome shotgun sequence.
ACCESSION   DAOMZY010000416 DAOMZY010000000
VERSION     DAOMZY010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39617_length_2621_cov_5.247077"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLK64_16325"
     CDS             complement(<1..180)
                     /locus_tag="PLK64_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81362.1"
                     /translation="MSNAASPGGTSEPNATGSGWLLRRIRDQQVAFLLVGATNTAVGF
                     LVFVGFDRLYAAVAPA"
     gene            complement(187..2181)
                     /locus_tag="PLK64_16330"
     CDS             complement(187..2181)
                     /locus_tag="PLK64_16330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPV81363.1"
                     /translation="MTGEPQWALASRVVFPVDHDEDLLPLYVEFGRRYPGVDDTHDEK
                     VRTWGEAKASGTPASAGIEGRSDDVLSRTSFAIRPGQRVSFATYFNAFPASYWRRWTT
                     AERVRLRVAVEGEATVLVYRSNARGSSIRLHRFTTGAPSANHPSERTEPTVVEVDLDL
                     VPFVDGGWYWFDIVAGGQPAVLASAEWLIFGEPQREARLSIGVTTVNKPEYVRKIVAA
                     IAGAHEVRQHLDKLYIVDQGTQAVQEQPGWDAAAEPLGTQLRVITQANLGGSGGFSRG
                     MYETLTAGSSTFHMVLDDDVSIEPESIVRAVRFASFARRPTIVGAHMFDIHNPTALHS
                     FGEEVDPVKWTFGAVRGVHEEHNLAFAGLRETPWLHRRVDVGYTGWWMCLIPVEVLRE
                     VGLSLPVFIKWDDAEHCMRARAAGFPTVSLPGSAVWHVSWNDKDDLIDWQAYYHQRNL
                     LISCLLHTTAPQGGQAIRQSIQALIKNAYSMRYYANAVRLQGLRDLFRGPEHLHATIG
                     ETLPQLRALAADYADVRFKADPDAFPKPIGQRLGPPPRLPKRSMLAPWVAKTAIRHLR
                     PTALGAQAAPQALVPQRYAVWWYLSQYDSAVVSKSDGTGAALYARQPETFRAQMAEGV
                     RLHVQLYRQWDALAKAYQDAVAEITSPAAWARTFGLDDPA"
     gene            complement(2178..2588)
                     /locus_tag="PLK64_16335"
                     /pseudo
     CDS             complement(2178..2588)
                     /locus_tag="PLK64_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879332.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
BASE COUNT          503 a          816 c          855 g          447 t
ORIGIN      
        1 ggcaggggcg acggcggcat acaaccggtc gaaaccgacg aagacgagga agccgacggc
       61 ggtgttggtg gcgcccacga ggaggaacgc cacctgctgg tcacgaatgc ggcgcagcag
      121 ccagcctgaa cctgttgcgt tcggttcgct cgtgccgcct ggactcgccg cgttgctcat
      181 cacacgtcac gccgggtcgt ccagcccgaa ggtccgcgcc caggccgccg gcgaggtgat
      241 ctcggccacg gcatcctgat aggccttcgc caacgcatcc cactgccgat agagctgcac
      301 atgcaggcgc actccctcgg ccatctgggc gcggaaagtc tccggttgac gggcatagag
      361 agccgcgccc gtaccatctg acttcgacac gacggcggag tcgtactgcg agaggtacca
      421 ccagacggcg taccgctgcg ggaccagcgc ctggggggcg gcttgggcac caagagcggt
      481 gggtctcaaa tgacggatgg ccgtctttgc gacccaaggg gcaagcatcg aacgcttggg
      541 cagccgcggg ggaggcccga ggcgctgacc gattggcttg gggaacgcgt cgggatcggc
      601 cttaaaacga acatcggcat agtccgccgc gagggcccgc aactggggca atgtttcgcc
      661 gatggtggcg tggaggtgtt ctggccctct gaacaagtcg cgtaggccct gcaagcggac
      721 cgcgttggcg tagtagcgca tcgagtaggc gttcttgatc agcgcttgga tggactggcg
      781 aatcgcctgc ccgccttggg gcgccgtcgt gtgcagcagg caagagatga gcaggttgcg
      841 ctggtggtag taggcctgcc agtcgatcaa gtcgtccttg tcgttccagg agacatgcca
      901 gactgcggac cccgggaggc tgaccgtcgg gaatcccgca gcgcgggccc gcatacaatg
      961 ctcggcgtcg tcccatttga taaacactgg cagcgacaaa ccgacctcgc gcaggacctc
     1021 cacggggatg aggcacatcc accatcccgt gtaaccaacg tccactcgcc gatgcagcca
     1081 tggcgtctcg cgcaggccgg cgaaagcgag attgtgctct tcgtggacgc cgcgcacggc
     1141 tccgaacgtc cacttcaccg ggtcgacctc ttccccgaag ctgtggaggg cggtcgggtt
     1201 gtgaatatcg aacatgtgag cgccgacgat cgtcgggcgc cgggcgaacg aggcgaagcg
     1261 tacggctcgg acgatgctct ctggctcaat cgagacgtcg tcgtcgagca ccatgtggaa
     1321 cgtgctcgat ccagcagtca gcgtttcgta cataccgcgg gagaagcccc cagatccccc
     1381 aaggttggcc tgagtgatga cacgcagttg cgtccccagc ggctccgcag ccgcatccca
     1441 gccaggttgc tcctggacgg cctgagtgcc ttggtcgacg atatagagct tgtccaggtg
     1501 ctgacgcacc tcgtgagcac ccgcgatggc agccacaatc ttgcgcacgt actcgggctt
     1561 gttgaccgtt gtcaccccga tcgagaggcg ggcctcacgc tggggttcgc cgaagatcaa
     1621 ccactcagcc gaggccagaa ccgctggctg ccctccggcg acgatgtcga accagtacca
     1681 ccctccgtcg acgaagggaa caaggtcgag gtcaacttca acgacggtcg gctcggtccg
     1741 ctcgctggga tggttggccg acggtgcgcc agtcgtgaag cggtgcagcc gaatcgagga
     1801 gccgcgggcg ttggaccggt agaccaggac cgtggcttcg ccctcgactg cgactcgcaa
     1861 ccgcacccgt tcagcggtcg tccatcggcg ccagtatgac gccgggaagg cgttgaagta
     1921 ggtcgcgaag gagacccgct ggccgggacg aatcgcgaat gacgtgcggg agaggacatc
     1981 gtcgctgcgc ccctcgatgc cggctgaggc gggggtgccg ctggccttcg cctcccccca
     2041 agtgcggact ttctcgtcgt gggtgtcatc cacgcctggg tagcgccggc cgaattcgac
     2101 atagagcgga agaaggtcct cgtcgtggtc aacaggaaac accacccgcg aggcgagggc
     2161 ccactgtggt tcaccggtca ctcagaagtc ctttcgtccc tcgggtcagc cggagcccca
     2221 ccgcgagaga aatgttcact caaatgatta tcgaccatcg acaacgctga cccgattgcc
     2281 atgtgcatat cgagatactg ataagtgccg agtcgcccac caaagaacac caaaggttcg
     2341 gcggcagcgt gctcacgata cctcagcaac cgagcgcggt cctgaggggt attgactgga
     2401 tagtagggct cgtcatcccg gtccgcgaat cgcgagaatt ctcgcataat cacagtgcga
     2461 tcggagggat agtcgcgctc cggatgaaag tgtcggaatt catggatccg agtgaagggc
     2521 acctcggggt ccggatagtt catcacggac gtcccctgga aatctcccac cgaaagcact
     2581 tcctgctcct agggtgcgcg ctgttcccaa ttgatctata g
//
LOCUS       DAOMZY010000417         2611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_39827_length_2611_cov_6.458138, whole genome shotgun sequence.
ACCESSION   DAOMZY010000417 DAOMZY010000000
VERSION     DAOMZY010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39827_length_2611_cov_6.458138"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..162
                     /locus_tag="PLK64_16340"
     CDS             <1..162
                     /locus_tag="PLK64_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPV81364.1"
                     /translation="ETFASRIANNAAEFDSPQKTGAFIAAGLVLFILTFVVNAIARVV
                     IERRKAFTE"
     gene            159..1139
                     /gene="pstA"
                     /locus_tag="PLK64_16345"
     CDS             159..1139
                     /gene="pstA"
                     /locus_tag="PLK64_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPV81365.1"
                     /translation="MSATSAAETAEATGTPLSRLVAARRAAYRADVVTDDPPQLTRGS
                     ASRRLTDQVARVVMWASFGLAMVPLVWILGTVVARGYELLLASSWWTLSQRNINAADV
                     GGGAVHAIQGTLIQAAVTAAISVPLGIFTAIYLVEYGRGRLARAVSFMVDILSGVPSI
                     VAALFVYAVWVTTFGFQRIGFAVCLSLVLLMIPVVVRSTEEMLKLVPNDLREGSYALG
                     VPKWITITRIVLPTAFSGIVTGVMLGLARVMGETAPLLILGPYTKSIATNLFDGYMPT
                     LPTMINQDRTEIGLDSAMERMWATALTLILIVLALNLVGRWAARRSSVRR"
     gene            1169..1945
                     /gene="pstB"
                     /locus_tag="PLK64_16350"
     CDS             1169..1945
                     /gene="pstB"
                     /locus_tag="PLK64_16350"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HPV81366.1"
                     /translation="MANRLKVEDVNIYYGAFKAVEGVTLTFEPRSVTAFIGPSGCGKS
                     TVLRTLNRMHEVIPGGRVEGSITLAGQDLYAAEVDPVAVRRTVGMVFQRPNPFPTMTI
                     FDNVVAGLRLNGLRNKSELADAAERSLRGANLWNEVKDRLNKPGAGLSGGQQQRLCIA
                     RAIAVEPHVLLMDEPCSALDPISTQAIEDLIEELKTDYMIVIVTHNLQQAARVSDQTA
                     FFNLAATGQPGKLVEVGPTPRIFEHPVHESTEAYVSGRFG"
     gene            complement(1991..2176)
                     /locus_tag="PLK64_16355"
     CDS             complement(1991..2176)
                     /locus_tag="PLK64_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81367.1"
                     /translation="MTEEQHDHEVTTPYSETLRAVADSEPLTWPADDTTVTETGDALA
                     ALAEDQLKAHMVVGRMY"
     gene            complement(2261..2407)
                     /locus_tag="PLK64_16360"
     CDS             complement(2261..2407)
                     /locus_tag="PLK64_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81368.1"
                     /translation="MSGSSAQGEIHDMAKLEEMELVDNLEMAAAAAPQRRLTAEEIDQ
                     ALGL"
BASE COUNT          389 a          911 c          862 g          449 t
ORIGIN      
        1 gagacgttcg cctcccgcat cgccaacaac gccgccgagt tcgactcgcc gcagaagaca
       61 ggcgcgttca tcgcagccgg cctggtgctg ttcatcctga ccttcgtcgt caacgccatc
      121 gcgagggtcg tcatcgagcg taggaaggcc ttcaccgaat gagcgcgacc agcgccgcag
      181 agaccgccga ggcgacgggc accccgctgt cccgtctcgt ggccgccagg cgcgcggcat
      241 accgagcgga tgtcgtgacc gacgacccgc cgcagctcac gcgcgggtct gcgtcccggc
      301 ggctgaccga ccaggtcgcg cgggtcgtca tgtgggcgtc cttcgggctc gcgatggtcc
      361 cgctcgtgtg gatcctcggc acggtggtgg cccgcggcta cgagttgctg ctggccagct
      421 cctggtggac cctgtcgcag cgcaacatca acgcggccga cgtgggcggc ggcgcagtcc
      481 acgcgatcca gggcaccctc atccaggccg cggtgaccgc ggcgatctcg gtgccgctgg
      541 gcatcttcac ggcgatctac ctcgtggagt acggccgcgg ccggcttgcg cgcgccgtgt
      601 cgttcatggt cgacatcctc agtggggtgc cgtccatcgt tgccgcgctc tttgtgtatg
      661 ccgtgtgggt caccaccttc ggtttccagc ggatcggctt cgctgtgtgt ctgtccttgg
      721 tgctgctgat gatcccggtg gtcgtccgct ccacggaaga gatgctcaag ctcgttccga
      781 acgacctgcg cgaagggtcg tatgcgctcg gggtgccgaa gtggatcacc atcacgcgca
      841 tcgtcctgcc gacggcgttc tccgggatcg tcacaggcgt catgcttggc ctggctcgag
      901 tcatgggcga aaccgccccg ctgctcatcc tcggccccta caccaagagc atcgccacca
      961 acctcttcga cggctacatg ccgacgctgc cgacgatgat caaccaagac cgcaccgaga
     1021 tcggcctgga cagcgccatg gagcggatgt gggccaccgc gctcaccctc atcctcatcg
     1081 tcctcgcgct caacctcgtc ggccgctggg ccgctcggcg ctcctccgtc cggcgctgac
     1141 gcgcccaccc ctgaaagcga gactccccat ggccaaccgg ctcaaggtcg aggacgtcaa
     1201 catctactac ggcgcgttca aggcggtgga gggcgtcacg ttgacgttcg aaccgcgctc
     1261 ggtcacggcg ttcatcggcc cctccggctg cggcaagtcg acggtgcttc gcacccttaa
     1321 ccgcatgcac gaagtgatcc ccggagggcg ggtggagggc agcatcaccc tcgccggcca
     1381 ggacctgtat gccgcggagg tcgatcccgt ggccgtgcgc cgcaccgtcg gcatggtctt
     1441 ccagcggcct aaccccttcc ccacgatgac gatcttcgac aacgtcgtcg ccgggctgcg
     1501 gctcaacggc ctgcgcaaca agtcggaact cgccgacgcg gccgagcggt cgttgcgggg
     1561 cgcgaacctc tggaacgagg tcaaggatcg gctgaacaag ccgggcgccg ggctgtctgg
     1621 aggccagcag cagcgtctgt gcatcgcccg cgcgatcgcc gtcgagccgc acgtgctgct
     1681 catggacgag ccgtgctcag cgctcgaccc gatctcgacg caggccatcg aggacctcat
     1741 cgaggaactc aagaccgact acatgatcgt catcgtcacg cacaacctgc agcaggctgc
     1801 tcgggtgtcg gatcagacgg cgttcttcaa cctggcggca accggtcagc ccgggaagct
     1861 ggtcgaggtc ggtccgacgc cgcggatctt cgagcacccc gtgcacgagt cgaccgaggc
     1921 ctacgtctct ggccgcttcg gctgatcagt ccgctcgccc cgctctccgt ggggcgagca
     1981 tccggcctgg tcagtacatc cggccgacga ccatgtgggc cttgagctgg tcctcggcga
     2041 gcgccgcgag cgcgtcaccc gtctcggtga ccgtcgtgtc gtcggcgggc caggtcaagg
     2101 gctcgctatc ggcaacggcg cgcaacgtct ccgagtaggg cgttgtcacc tcgtggtcgt
     2161 gctgctcctc ggtcatgagt gcagcgctcc ttcccccagt gcgggccacg gtgcccggtc
     2221 ctttgagagt agacccggca tcgacgatcc gccacagggt tcacaggccg agggcttggt
     2281 cgatctcctc ggcggtcagg cgacgctgcg gcgcggcagc ggccgccatc tcaaggttgt
     2341 cgaccaactc catctcctcg agtttggcca tgtcgtgaat ttccccctgg gcgctcgatc
     2401 cgctcatggg ggcagagtag ggaacggcgg gcccgcagga aggcgatcgc catcgtcggc
     2461 cgagggcgtc gccggacggt ccggccccga cgacgaacgg tcgacggttg tgtgattgct
     2521 gtgggtgcgt cgggccggga gcgcctcacg gcgcaaggcc gctcgtagcg gctatttgtg
     2581 ggacccgggg ctaccgcggc ccgacgcaga c
//
LOCUS       DAOMZY010000418         2606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_39950_length_2606_cov_6.947080, whole genome shotgun sequence.
ACCESSION   DAOMZY010000418 DAOMZY010000000
VERSION     DAOMZY010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39950_length_2606_cov_6.947080"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..2306
                     /gene="gyrB"
                     /locus_tag="PLK64_16365"
     CDS             177..2306
                     /gene="gyrB"
                     /locus_tag="PLK64_16365"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPV81369.1"
                     /translation="MVDPTTPIPADVTDTGETAYDASAITVLEGLEAVRKRPGMYIGS
                     TGERGLHHLVWEIVDNAVDEALAGYADTIDVTLRADGGVRVKDNGRGIPTDIHPTEGV
                     SAVELVLTQLHAGGKFGGGGYKVSGGLHGVGSSVVNALSSRLDVEVRQKGNAFRMSFD
                     HGVPVAPLAKQEETDETGTTVTYWASEDIFDTTHYDFETIRARFQQMAFLNKGLRINL
                     VDERIDPDRVEDTDDVDLDNLDAGVEKVARTDDGVGAAEAEGGASAGSAAGSAAVAKA
                     RKVSYRYDGGLVDYVKHLVGSKKSDPIHPEIISIESEDATRSLSLELAMQWTTAYSES
                     VHTYANTINTHEGGTHEEGFRAAMTKLVNEFARRQGQLKDKDENLTGDDIREGLTAVV
                     SVKLGEPQFEGQTKTKLGNSEVKGFVQSAMTSEFGHWLEAHPREGKDIVGKAIQASLA
                     RIAARKAREATRRKSPLESGGLPGKLRDCQTKDPSESEIFIVEGDSAGGSAVQGRDPN
                     SQAILPIRGKILNVEKARIDKILANTEVQALVSAFGTGIGEDFDLAKARYHKIVLMAD
                     ADVDGMHIRTLLLTLLFRFMRPLIDAGYVYLAQPPLYRIKWSNSPHEFAYSDRERDGL
                     IAQGEGKGRRMPKENGVQRYKGLGEMNPAELRDTTMDPDTRTLLQVTLADAAAADEIF
                     SILMGEDVESRRGFIQRNARDVRFLDI"
     gene            2337..>2606
                     /locus_tag="PLK64_16370"
     CDS             2337..>2606
                     /locus_tag="PLK64_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81370.1"
                     /translation="MSDSGQSSAPTRVAGSTAGVCREQTIRALSLRSAVRGAGSTAEV
                     CRDRTIRARSLRSLALPPGPPRSRHTPAASRLSVTVEARHTPAASW"
BASE COUNT          462 a          912 c          851 g          381 t
ORIGIN      
        1 aatcgactct cagggccgct aacggcccgt gttgagcact catggtcggt gcggggcact
       61 agactggcct tctacggctg ccgctagtgc gacaaacgcg gcagccgtcg gcatgacttc
      121 gtgccgacga gcgcccggcc cgtccgggag gccgtcggcc gaccgacagg agcaccgtgg
      181 tcgaccccac caccccgatc cccgccgacg tcaccgacac tggcgaaacg gcatacgacg
      241 cctcggcgat caccgtcctc gaggggctgg aggccgtccg caagcggccc ggcatgtaca
      301 tcgggtcgac cggtgagcgc ggcctgcacc acctcgtgtg ggagatcgtc gacaacgctg
      361 tggacgaggc gctcgctggg tatgccgata cgatcgacgt cacgctgcgc gccgacggcg
      421 gggtgcgggt caaggacaac gggcgtggca tccccaccga catccacccg accgagggcg
      481 tcagcgccgt cgagctcgtg ctcacccagt tgcacgctgg cggcaagttc ggcggcggcg
      541 gctacaaggt gtccggcggt ctgcacggcg tcggctcgtc tgtcgtcaac gccctgtcca
      601 gtcgccttga cgtcgaggtc cgccagaagg gcaacgcctt ccggatgagc ttcgaccacg
      661 gcgtccccgt ggcgccgttg gccaagcagg aagagaccga cgagaccggc acgaccgtca
      721 cctactgggc gtccgaagac atcttcgaca cgacccacta cgacttcgag acgatccgcg
      781 cgcgattcca gcagatggcc ttcctcaaca agggcctgcg gatcaacctc gtcgacgagc
      841 ggatcgaccc cgaccgggtc gaggacaccg acgacgtcga cctggacaac ctcgacgcgg
      901 gcgtcgagaa ggtcgcccgc accgacgacg gtgtcggtgc tgctgaggcc gagggcggcg
      961 cctccgccgg gtcggctgcc gggtcggccg ccgtggccaa ggcccggaag gtgtcctacc
     1021 gctacgacgg cggcctggtc gactacgtca agcacctggt cggttcgaag aagtccgacc
     1081 cgatccaccc cgagatcatc tccatcgagt ccgaagacgc gacccgctcg ctctcgctcg
     1141 agttggcgat gcagtggacc acggcctaca gcgagtcggt ccacacctat gccaacacca
     1201 tcaacaccca cgagggcggc acccacgaag agggcttccg cgcggcgatg accaagctgg
     1261 tcaacgaatt cgcccgccgc caggggcagt tgaaggacaa ggacgagaac ctcaccggcg
     1321 acgacatccg cgagggcctg accgccgtcg tcagcgtcaa gctcggtgag ccgcagttcg
     1381 aggggcagac caagaccaag ctgggcaact ccgaggtcaa ggggttcgtt cagagcgcca
     1441 tgaccagcga gttcggtcac tggctcgagg ctcacccgcg cgaaggcaag gacatcgtcg
     1501 gcaaggccat ccaggcctcg cttgcccgga tcgccgcccg caaggcgcgc gaggcgaccc
     1561 ggcgcaagag cccgctggag tccggtggac tgcccggcaa gctgcgcgac tgccagacca
     1621 aggacccgag cgagtcggag atcttcatcg tcgagggtga ctcggccggt gggtcggcgg
     1681 tccagggtcg cgaccccaac agccaagcga tcctcccgat ccgcggcaag atcctcaacg
     1741 tcgagaaggc ccgcatcgac aagatcctcg ccaacaccga ggtccaggcg ctcgtttcgg
     1801 cgttcggcac cggcatcggt gaggacttcg atctggccaa ggcgcgctat cacaagatcg
     1861 tcctcatggc cgatgccgac gtcgacggca tgcacatccg gaccttgctg ctgacgctgc
     1921 tgttccgctt catgcgcccg ttgatcgacg ccgggtacgt ctacctggcc cagccgcccc
     1981 tctaccggat caagtggtcg aactcgccgc acgagttcgc ctactccgac cgcgagcgcg
     2041 acggtctcat cgctcaaggc gagggcaagg gtcggcggat gccgaaggag aacggcgtcc
     2101 agcgctacaa gggtctgggt gagatgaacc cggccgagtt gcgcgacacg accatggacc
     2161 ctgacacccg gacgctcttg caggtgaccc tcgctgatgc tgcggccgcc gacgagatct
     2221 tttcgatcct catgggcgag gacgtcgagt cccgccgcgg gttcatccag cgcaacgccc
     2281 gcgacgtgcg gttcctggac atctgacgat ctagcgagga acgagcgatg aggaagatgt
     2341 ccgattcagg gcagtcatct gcgccgacgc gcgtggctgg gtcgacggca ggggtatgcc
     2401 gcgagcagac catccgcgct ctctcgctgc gctcagccgt gcgcggggct gggtcgacgg
     2461 cagaggtatg ccgcgaccga accatccgcg ctcggtcgct gcgctccctc gcgctcccgc
     2521 caggcccgcc acggtcgcgg catacccctg ccgcctcccg tctctctgtc accgttgagg
     2581 cgcggcatac ccctgccgcc tcctgg
//
LOCUS       DAOMZY010000419         2591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_40281_length_2591_cov_4.835174, whole genome shotgun sequence.
ACCESSION   DAOMZY010000419 DAOMZY010000000
VERSION     DAOMZY010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40281_length_2591_cov_4.835174"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /locus_tag="PLK64_16375"
     CDS             <1..1434
                     /locus_tag="PLK64_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPV81371.1"
                     /translation="VVVGVPVAHALYRLALPGRRTLRAIVAMPFVLPTVVVGVMFRSL
                     LAEGGALGGLGLDGEWGGILAAFVFFNLAVVVRTVGAAWEGLDRRPEEAAATLGAGPA
                     TVFRTVTLPALAPSIISAASIVFLFCATAFGVVLTMGGLAYGTVETEIYLLTTALFDL
                     RGAAVLSVVQLVAVVAMLLLADRARSRDAGTQRAAKSAIAPGRVTAYAMGSVIATVLA
                     VAFVAVPVLALVVRSLRRQGEWTLDYYLALGRVGGADGRSALPVTVWEALGTSWRIAI
                     DATILAVVLGLLVAVLVSRPARSPGGLRWRRLLDGVVMLPLGVSAVTVGFGFLITLDR
                     PPLDLRTSPVLIPIAQAIVALPLVVRTLAPVLRAIDDRQRQVAATLGARPWQVLATVD
                     LPIIWRPLLAAVGLALAVSLGEFGATSFLARPDSPTLPVVIYRLIGRPGVDNLGMALA
                     ASVILAVVTVTVMGVVERLRVSSVGAF"
     gene            1434..2093
                     /locus_tag="PLK64_16380"
                     /pseudo
     CDS             1434..2093
                     /locus_tag="PLK64_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147487.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            2230..>2591
                     /locus_tag="PLK64_16385"
     CDS             2230..>2591
                     /locus_tag="PLK64_16385"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005266941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPV81372.1"
                     /translation="MRLLLIRHGQTASNVTRLLDTAAPGAPLDEVGLEQARALAEALA
                     DEPIEAVYASDLTRSQQTAAPLAARHGVEVVVRGGIREIQAGEDEMSADWVRYLTTIM
                     SWNADLDARMPGGESGREV"
BASE COUNT          273 a          899 c          957 g          462 t
ORIGIN      
        1 gtcgtcgtcg gggtgccggt ggcccacgcc ctctatcggt tggccctgcc cggccgtcgg
       61 acgctgaggg ccatcgtcgc gatgcccttc gtcctgccga ccgtcgtcgt cggcgtgatg
      121 tttcggtcgt tgctcgccga gggcggcgcg ctcggtgggc tcgggctgga cggggagtgg
      181 ggcggcatac tcgccgcctt cgtgttcttc aacctcgcgg tcgtcgtgcg caccgtcgga
      241 gcggcctggg agggcttgga tcgccggccc gaggaggccg cggccaccct gggcgccgga
      301 cccgcaacgg tcttccgcac cgtgacgctg cccgccctgg caccgtcgat catctcggcg
      361 gcgtccatcg tcttcctttt ctgcgccacg gcttttggtg tcgtcctgac gatgggcggg
      421 ctggcctacg gcaccgtcga gaccgagatc tacctgctga cgactgccct gttcgacctg
      481 cgcggggcgg cggtgttgtc ggtcgtccaa ctcgtcgccg tcgtcgccat gctgctgctg
      541 gccgatcggg cccgctcccg cgacgccggg acccagcggg ccgcgaagtc cgcgatcgcg
      601 ccggggcggg tcacggcata cgcgatgggc tcggtgattg ccacggtgct ggcggtcgcg
      661 ttcgtcgcgg ttcccgtgct ggcgttggtg gtgcgctcgc tgcggcgcca gggggagtgg
      721 accctggact actacctcgc gttgggacgg gtcggcgggg ctgacggtcg cagcgcgctg
      781 ccggtcaccg tgtgggaggc gctgggcacg tcgtggcgga tcgccatcga tgcgacgatc
      841 ctcgccgtcg tcctcggcct gctcgtcgcc gtcctcgtct cgcgcccggc ccgctcgccg
      901 ggcggtctgc gatggcgccg gttgcttgac ggggtggtca tgctgccgtt gggggtgtct
      961 gcggtgacgg tcggcttcgg cttcctcatc acgttggacc ggccgccgtt ggacctgcgg
     1021 acttcgccgg tgctcatccc gatcgcccaa gcgatcgtcg ccctgcccct ggtggtgcgg
     1081 accctcgcgc cggtgctgcg ggctatcgac gaccggcaac ggcaagtcgc cgcgacgttg
     1141 ggtgctcgcc cgtggcaggt actcgccacc gtcgacctgc cgatcatctg gcggccgttg
     1201 ctggcggccg tcgggctggc gctcgcggtc tccctggggg agttcggtgc gacgagcttc
     1261 ctggcccgac ccgacagccc gaccctcccc gtggtcatct atcgcctcat cgggcggccg
     1321 ggggtcgaca atctcggcat ggcactcgcg gcgtcggtca tcctcgccgt tgtcaccgtc
     1381 accgtcatgg gtgtcgtcga gcgcctgcgg gtgtcgtcag tgggggcctt ctgatgctct
     1441 cgatcagccg tgtgtcggtg tctttcgggg cgacccgcgc ggtcgacgat gtcagccttg
     1501 aggtcccctc gggcacggtg atggcgatcc ttgggccgtc agggtgcggc aagtccagcc
     1561 tgctgcgggc cgtcgccggg ctcgaaccgc tcgcgtcggg ctcggtgagc ttcgacgggt
     1621 cggacctcgt gggggtgccg acccatcggc gcggcttcgc gatgatgttc caggacgggc
     1681 agctgttcgg tcacctcgat gtcgccggga acgtcggtta tgcgttggcg cgcaagggga
     1741 tccgtggcgg gcaggcgcgc acggaggtcg gccgcctcct cgagctcgtc ggcctcgcgg
     1801 ggtatgccgc gcgccgcccc tccaccctca gcggcggtga gggtcagcgc gtcgcgttgg
     1861 cccgggcgct ggctgcccag ccccggctgc tgttgctcga tgagccgctg tcggcattgg
     1921 atcggtcgct gcgcgagcgc ctggcgaccg acctgcggca cgtgctcact gagaccggga
     1981 cgacggcaat gatggtgacc cacgaccatg gcgatgcctt cacggtggcc gatcggacgg
     2041 cgatcatgcg agcggggcgg ctggtgcagc aaggtgccac ggccgaggtc tgggcgcacc
     2101 ccgtcgatgc cgacaccgcc cacttcctcg gcgtcgctgt cccctcgggc ctcgcggagc
     2161 cctgagctca aaccgctgcg tggcgataac cgcgccgagc gcggggccgg gagcggctag
     2221 cctcggtggg tgcgactctt gctcatccga cacggccaga ccgcgtcgaa cgtcacccga
     2281 ctgctcgaca ctgcggcccc cggtgcccca ctcgacgagg tcggcctcga gcaggcccgg
     2341 gccctggccg aggcgctcgc cgacgagccg atcgaggccg tctatgcctc cgacctgacc
     2401 cgcagccagc agacggctgc gccgctggcc gctcgccacg gtgtcgaggt cgtcgtccgt
     2461 ggcggcatcc gggagattca ggcgggcgag gacgagatgt ccgccgactg ggtccgctac
     2521 ctcacgacga tcatgtcctg gaacgccgat ctcgacgctc ggatgccagg cggggagtcg
     2581 gggcgggagg t
//
LOCUS       DAOMZY010000420         2583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_40471_length_2583_cov_5.260285, whole genome shotgun sequence.
ACCESSION   DAOMZY010000420 DAOMZY010000000
VERSION     DAOMZY010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40471_length_2583_cov_5.260285"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PLK64_16390"
     CDS             <1..334
                     /locus_tag="PLK64_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81373.1"
                     /translation="DLSLSGHDHNISRAYQIGGPVTSQTSPTVVDRDGSFVSGAGTVF
                     AVLGTGGHSPREIGSLGSIWAMGSGTNSPGGISFGFGEITVSGNTLTYQLRTVSGPKL
                     TDVFTIIR"
     gene            complement(441..514)
                     /locus_tag="PLK64_16395"
     tRNA            complement(441..514)
                     /locus_tag="PLK64_16395"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(478..480),aa:Ile,seq:cat)
     gene            828..1337
                     /locus_tag="PLK64_16400"
     CDS             828..1337
                     /locus_tag="PLK64_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81374.1"
                     /translation="MKKQTLVSAVAAVATAAVLGMGGAALAQAGQSVPTSVPTSVQAD
                     ASPSATATPGAPAGQGRERAPGLTKEKLKDWIAKHGLTGDTAVKVTEAAVAKEPTAYV
                     LRVGKLADGTYVARMMRPDGTRIVLMIDANFAVTSVEDAPEKVPGQGRGAGKGAKNNA
                     STASPTASS"
     gene            1533..2048
                     /locus_tag="PLK64_16405"
     CDS             1533..2048
                     /locus_tag="PLK64_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3145 domain-containing protein"
                     /protein_id="HPV81375.1"
                     /translation="MSVTASRNATRGVVFIHSTPTALCPHITWALENVLGQPLHLDWT
                     IQPAGPRMVRAEVAWTGDPGLGARLASALRGWDHVRYEVTEEPSPGVDGSRWSHTPTL
                     GLHHTWTSASGDAMVNEDRLRDVFETHRGDVPALHAAIDALLGAPWDRELEAFRYAGD
                     GAPVRWLHKVG"
     gene            complement(2137..>2583)
                     /locus_tag="PLK64_16410"
     CDS             complement(2137..>2583)
                     /locus_tag="PLK64_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775235.1"
                     /note="FabF; beta-ketoacyl-ACP synthase II, KASII;
                     catalyzes a condensation reaction in fatty acid
                     biosynthesis: addition of an acyl acceptor of two carbons
                     from malonyl-ACP; required for the elongation of
                     short-chain unsaturated acyl-ACP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HPV81376.1"
                     /translation="AHHITAPEPEGAGASRAMLEAVSTAGGTLRDVVHINAHATSTPV
                     GDVAETNAIRRAFGDLADSIPVSATKSMTGHLLGAAGALEAIFTILALHHRLAPASIN
                     LDTMDPQISLDVVHGSHRQLPAGDLLALDNSFGFGGHNVALAFRTV"
BASE COUNT          442 a          852 c          903 g          386 t
ORIGIN      
        1 tgacctatca ctgtctgggc acgaccacaa catcagccgt gcctatcaga tcggagggcc
       61 cgtcacatcg cagacctctc ccacagtcgt ggacagggac ggatcgttcg tctccggcgc
      121 agggactgta ttcgccgtcc tcggcactgg tgggcactcg ccaagggaga tcggctcttt
      181 gggctccatc tgggccatgg gctcggggac caacagtcct ggaggtatct cgttcgggtt
      241 cggtgagatc accgtgagtg gcaacaccct cacgtatcaa ctccggactg tcagcggccc
      301 gaagctgacg gacgtcttca cgatcattcg atgatgcgtg tgaccctgcg gcgctctcgg
      361 tatcgcaggg tcatgctaag cgaagcggtg gcggacggac gtgcaatcgg ccgtcatacc
      421 tctgacctgc ggttatatcg tggggcgggt ggggcttgaa cccacgaccg acggattatg
      481 agtccgctgc tctgaccggc tgagctaccg ccccgcaccc ctcggggcct ggccatccga
      541 cgctcagcgc gcgggcggca tatcgatgag ggggtcgttc gtgcggacgc gagcctaccc
      601 gtcccagggg gaggtcaggg actcaccgcc gcctgcccat ggaagcgaca cccgttcaca
      661 accgcttaga cggtcaccct gacgcgagac catcgacgag gaggacagtg ccatgcgctt
      721 catgccgcgc agagcgagtc acaggttctt gacaggattg tcacgacggg ccgtaacacg
      781 gctgttcgca tgatgtggac accagacgcc acacaaagga gccgtcagtg aagaagcaga
      841 ctctcgtgag cgcagtcgcc gcagtggcga ccgcggcagt gctgggcatg ggcggcgcgg
      901 cgctcgccca ggcgggccag tcggtgccca cctctgtccc cacgtcggtg caggccgacg
      961 caagcccctc ggcgaccgcg acgccgggag cacccgcggg ccaagggcgc gagcgtgccc
     1021 ccggcttgac caaggagaag ctcaaggact ggatcgccaa gcacggtctc accggcgaca
     1081 ccgccgtcaa ggtcacggag gcagccgtcg ccaaggagcc gacggcctac gtgctgcggg
     1141 tcggcaagtt ggctgacggg acctacgtgg cgcgaatgat gcgaccggac gggacccgga
     1201 tcgtcctcat gatcgacgcc aacttcgccg tgacgtcggt cgaggacgct ccggagaagg
     1261 ttcccggaca gggtcggggt gcgggcaagg gcgccaagaa caacgcgtcg accgcgagcc
     1321 cgaccgcatc gagctaaccg ctctagaact ccacacacat ctcccgcacc aggccgcgct
     1381 ggtgcgggag atgtgtttgg ggcagaatgt gactcacgtg acgcgtgtcg caccaccaag
     1441 tgagcgacac cctccgagcg caagccagca ccacggcata ccgatcgagc gagacgttgg
     1501 ggaagacgtc cctcgcacgt gaggagtacc ggatgtctgt caccgcgtcc cggaacgcga
     1561 cgcgcggggt cgtgttcatc cactcgaccc ccactgcgtt gtgcccgcac atcacctggg
     1621 ccctggagaa cgtcctcggc caacccctgc acctcgactg gacgattcaa ccggccggac
     1681 cgcggatggt ccgagccgag gtcgcctgga cgggtgaccc gggccttggc gcccggctgg
     1741 ccagtgcgct gcggggctgg gaccacgtgc gctacgaagt gaccgaggag ccctcaccgg
     1801 gcgtcgacgg ctcacgctgg tcgcacaccc ccaccctcgg gttgcatcac acctggacct
     1861 cggccagtgg cgacgccatg gtcaacgagg accggctgcg cgatgtcttc gagacccacc
     1921 gcggggacgt ccccgcgctg cacgcggcca tcgacgcgct cctcggcgcc ccgtgggatc
     1981 gcgagttgga ggcattccgg tatgccggtg acggggctcc cgtgcgctgg ctccacaagg
     2041 tgggctgacc ggggccaccc gctctggcgc cgactccggt caggcgccac cgcccagcgg
     2101 ccaaaggctc gctgggcggt gggcgatcga ggggcctcag acggtgcgga aggcgagggc
     2161 cacgttgtgc ccgccgaagc cgaacgagtt gtccaaggcc aacaggtcgc cggccggcaa
     2221 ctgccggtgc gatccgtgga cgacgtcgag ggagatctgc gggtccatcg tgtcgaggtt
     2281 gatcgaggcg ggggcgagcc ggtgatgcag ggcgaggatc gtgaagatgg cctccagcgc
     2341 cccggcggca ccgagcaggt gtccggtcat cgacttggtg gcactgaccg ggatggaatc
     2401 ggccaggtcg ccgaaggcgc gacggatcgc gttggtctcg gccacgtcgc cgactggggt
     2461 cgaggtcgcg tgggcgttga tgtggacgac atcgcgcagg gtgcccccgg cggtcgacac
     2521 agcctccagc atggcgcggg aggccccggc gccctcgggc tcgggagcgg tgatgtggtg
     2581 ggc
//
LOCUS       DAOMZY010000421         2577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_40610_length_2577_cov_5.179223, whole genome shotgun sequence.
ACCESSION   DAOMZY010000421 DAOMZY010000000
VERSION     DAOMZY010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40610_length_2577_cov_5.179223"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLK64_16415"
     CDS             complement(<1..183)
                     /locus_tag="PLK64_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPV81377.1"
                     /translation="MDLHLTSPANPRLKALVGLRRRRARDEAGLTVVEGHEELALALA
                     AGVAPQTLYVCSELFSP"
     gene            complement(183..962)
                     /locus_tag="PLK64_16420"
     CDS             complement(183..962)
                     /locus_tag="PLK64_16420"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPV81378.1"
                     /translation="MIALDVDGTIITHAGHLDDRVRDAIEGCVHDYQHVVIATGRSVV
                     ATMPVLARLGITQGYAVCSNGAVTLRIDASRPKGYKILEKVTFDPRPALTLLREELPE
                     ALVAVEDLGRGFLVSSPFPDGELMGDQRVVPWDKLVGRRATRVTLRMPDAEPEAFMAH
                     IERIGLHEVSYAVGWTAWLDINPEGVSKGSALELLRRRLGVEPCDTVAVGDQRNDIEM
                     LQWAARGVAMGNAPDEVKAIADEVTGSVDEAGLAAVLESLR"
     gene            complement(1031..2314)
                     /gene="serS"
                     /locus_tag="PLK64_16425"
     CDS             complement(1031..2314)
                     /gene="serS"
                     /locus_tag="PLK64_16425"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPV81379.1"
                     /translation="MIDIKLVRDNPDVVRRSQQARGEDPAIIDAILAADETRRSSLAQ
                     FEAARAEQKSFGKQVAAAAPADKPALLAQVKDLAERVKALDAAAATAGEDAVRLLASV
                     PNVVLDGVPTGGEEDYVVRETVGTPRDFAAEGFEPRDHVALGELLGAIDIERGAKVAG
                     ARFYFLTGVGARLELALLNLAVARAVDAGFTPMITPTLVRPEIMRGAGFLDHHSDEVY
                     YLPADDLYLTGTSEVALSGYHRDEIIDLADGPKRYAGWSACYRREAGSHGKDTRGIIR
                     VHQFHKVEMFVYCRPEDAEAEHERLLGMERAMLDACELPYRIIDTAAGDLGDPAARKF
                     DCEGWVPTQGRYRELTSTSNCTTFQARRLGVRERDPQASGGATRIVATLNGTLATTRW
                     IVAILENHQQADGSVVVPEALRPFMGGLAVLEPVR"
BASE COUNT          377 a          918 c          910 g          372 t
ORIGIN      
        1 ggggctgaac aactctgagc agacatagag cgtctgcggc gccaccccgg ccgccagcgc
       61 caacgccagc tcctcgtggc cctcgacgac cgtcaggccc gcctcgtcgc gcgcgcgccg
      121 acgccgcaac cccaccagcg ccttgaggcg cgggttggca ggggaggtca gatgcaggtc
      181 catcagcgca gcgactcgag caccgcagcc aggccggcct cgtcgaccga gccggtcacc
      241 tcgtcagcga tcgccttgac ctcatcaggc gcgttgccca tcgccacccc acgagcagcc
      301 cactggagca tctcgatgtc gttgcgctgg tcgccaacgg cgacggtgtc gcacggctcg
      361 acgcccagcc ggcgccgcaa caactccagc gccgagccct tggagacccc ctcggggttg
      421 atgtccagcc aggcggtcca gccgacggca tacgagacct cgtgaaggcc gatccgctcg
      481 atgtgcgcca tgaacgcctc gggctcggcg tcgggcatcc gcagcgtgac acgcgtggcg
      541 cgccgcccca cgagtttgtc ccacggcacg acccgctggt cgcccatgag ctcgccatcg
      601 gggaaagggg aactgacgag gaagccgcgc cccaggtctt cgaccgccac gagcgcctcg
      661 ggcaactcct cccgcagcaa cgtcagcgcc ggccgcgggt cgaaagtgac cttctcgagg
      721 atcttgtacc ccttgggccg ggacgcgtcg atccgcagcg tcaccgcacc gttggagcac
      781 acggcatacc cctgggtgat ccccagccgc gcgaggaccg gcatcgtcgc gacgaccgag
      841 cgaccggtcg cgatgacgac gtgctggtag tcgtgcacac acccctcgat cgcgtccctc
      901 acccgatcgt ccaggtggcc ggcgtgggtg atgatcgtcc cgtcgacgtc gagcgcgatc
      961 aggcgacgac gaaaccccgg gtatgccgcg ggctttccgc cgcggccccg gccccccttg
     1021 gctcccttgg tcaccggacc ggctcgagga cggccaaccc gcccatgaag ggccgcaacg
     1081 cctcgggcac gacgaccgag ccgtcggcct gctggtggtt ctcgaggatc gcgacgatcc
     1141 accgggtcgt tgccagggtc ccgttgaggg tggcgacgat ccgggtcgcc ccgccgctgg
     1201 cctgcgggtc gcgctcgcgg accccgagac gacgggcctg gaaggtggtg cagttggacg
     1261 tcgaggtcag ctcgcgatag cggccctggg tgggcaccca gccctcgcag tcgaacttgc
     1321 gggcggccgg gtcgccgagg tcgcctgcgg cggtgtcgat gatccggtag ggcagctcgc
     1381 aggcgtcgag catcgcccgc tccatgccca gcaggcgctc gtgctcggcc tcggcgtcct
     1441 cgggacggca gtagacaaac atctcgacct tgtggaactg gtggacccgg atgatcccgc
     1501 gagtgtcctt gccgtggctg ccggcctcgc ggcggtagca ggccgaccac ccggcatacc
     1561 tcttggggcc gtcggcgagg tcgatgatct cgtcgcggtg gtagcccgag agcgccacct
     1621 cggaggtccc cgtgaggtaa aggtcgtcgg cggggaggta gtagacctcg tccgagtggt
     1681 ggtcgaggaa gccggcaccg cgcatgatct cgggccgcac cagggtcggg gtgatcatcg
     1741 gggtgaaacc cgcgtcgacg gcccgcgcca cggcgaggtt gagcagcgcg agctccagcc
     1801 tggccccgac gccggtgagg aagtagaacc gggcaccggc gaccttggcg ccgcgctcga
     1861 tgtcgatggc cccgagcaac tcccccaggg cgacatggtc gcggggctcg aagccctcgg
     1921 cagcgaaatc ccttggggtg ccgacggttt cgcgaacgac gtagtcctct tcgcctccgg
     1981 tcggcacgcc gtcgaggacg acgttgggga cgctcgcgag cagccgaacg gcatcttcgc
     2041 cggccgtggc agcggcggca tccagcgcct tgacccgctc ggcgaggtcc ttgacctggg
     2101 ccagcagggc cggcttgtcg gcgggcgccg ccgcggcgac ctgcttgccg aaggacttct
     2161 gctcggcgcg ggcggcctcg aactgcgcga gggacgaccg gcgggtctcg tcggcagcga
     2221 ggatcgcgtc gatgatcgcg gggtcctcgc cgcgggcctg ctgcgagcgg cggaccacgt
     2281 cggggttgtc acggacgagc ttgatgtcga tcacgcccgc cagcctaccg aggccccggc
     2341 caccgcctcg gcccagtttc atgcaggcgg tgagcgggcg gcatacccct ggggtatgcc
     2401 ggccacctcg gtcagtggga gcgcggccgg gtcagccgcg cacgtcccag acgtggtggc
     2461 gcaggtcgtg caacgcgtag acgccgatgg tggcgacggt gaactccgag ccgttgctgc
     2521 gggtgccgcg ccgctgccag tcttccttgc tcacccgctc gaacaggtcg gccagtt
//
LOCUS       DAOMZY010000422         2567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_40840_length_2567_cov_5.055732, whole genome shotgun sequence.
ACCESSION   DAOMZY010000422 DAOMZY010000000
VERSION     DAOMZY010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40840_length_2567_cov_5.055732"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2567)
                     /locus_tag="PLK64_16430"
     CDS             complement(<1..>2567)
                     /locus_tag="PLK64_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81380.1"
                     /translation="AQLPEHTTVYAESSGGLGTYAVSITVNGVRTDDDRAPVTTPPAG
                     SISVAVALTAVGGGPVVTTRTITVSRSATPVTAGASGTPVTVRTTSSEAAYLVLVEPS
                     TPTSARVRLSSGAAATWAWPGGTITGTEAEVPVAQGATVAVTAVTTAGQVQTLDAYYL
                     FDRPKVADGDAYAVNPANTHIGPATDRTHHSASALFIDHARSRLSVIGAATQLTVSGF
                     ASYEGDSTSAQEQRNRALSARRRDGMIAALHSVGYTNVVAGSADGTAAARAGTSIDGQ
                     PAPAAGSASWWRARAVSADPPASITVTGELTRPAPTPPARTDPTPTRSQPPDCFHKIG
                     VRVELVRSTFVRCEIYGEFDIETATEASLRRRGQPALRSGPRNAADGVCTFLVRLRIA
                     EDQGAWEVSGEFRAKEGDLDGLAEMTDSNANQVALDILGALSILAPLSASATELSPAA
                     GAVVALGSIALGASDLLHTHRLTLRGAEIIVSQGILGADGITTVSQRGTQVTVLVDIE
                     AAFSFDLTLVRVDPAHPIVTRYKAIGLRSSWDTVPAGGGGVDYVPLPVFFPDRGYTLD
                     IPAGSLTAAPPLDNILRILGVRVSRDNPTYLEVEVGLGLDLGVITIETVRVRARVDGP
                     PLDLQLTKFGASVDIPNVITGRGYLSFETGGFSGSFDLQIIPLKLRASASLAVRQAGG
                     VTGVLLGIEVEFPVPLVLGSSGLGLFGILAGVGINHGRVEDPSAPVPALKWLQDQFAR
                     PGGVMDPAGWSLQPGHYAFAAGVLLGTLEGGYIVHLKGIVLIEVPGPRLLFVLKADLI
                     KAPPALKSNQSATFLAVLDLDFGRGTITIGVVAAYEIESILTVRVPVTAFFSTQQVQ"
BASE COUNT          372 a          849 c          981 g          365 t
ORIGIN      
        1 ttctgcacct gctgggtgga gaagaacgcg gtcaccggca cccggacggt gaggatcgac
       61 tcgatctcgt atgccgcgac gacaccgatg gtgatcgtgc ctcgcccgaa gtccaggtcg
      121 aggacggcga ggaaggtcgc gctctggttg gacttgagtg ccggcggggc cttgatgagg
      181 tcggccttga ggacgaagag cagtcgtggc ccaggcacct cgatgaggac gatgcccttg
      241 aggtggacga tgtagccgcc ttcgagcgtc ccgaggagca cgccggccgc gaaggcgtag
      301 tggccgggct gcaacgacca ccccgccgga tccatgacgc cgccgggtcg ggcgaactgg
      361 tcctgcaacc acttgagcgc aggcaccgga gcactcgggt cctcgacccg cccgtggttg
      421 attccgacgc cggcaaggat gccgaagagc ccgagccctg aactgcccag cacgagcggc
      481 acggggaact cgacctcgat gcccagcaac actccggtga ccccacctgc ctggcggacg
      541 gcgaggctgg cgctggcgcg cagcttgagg gggatgatct gcaggtcgaa cgacccggag
      601 aagccgcccg tctcgaagga caggtaaccg cgcccggtga tgacgttggg gatgtcgacg
      661 ctggccccga acttggtgag ctgcaggtcc agtggcggac cgtcgacccg cgcgcggacc
      721 cggacggtct cgatggtgat gacacctagg tccagcccca gaccgacctc gacctcgagg
      781 taggtcgggt tgtcgcggct gacccgcacg ccgaggatcc gcaggatgtt gtccagcggc
      841 ggggcggcgg tgaggctgcc cgccgggatg tccagggtgt agccccggtc ggggaagaac
      901 acgggcaggg ggacgtagtc gaccccgccc ccacccgcag gcacggtgtc ccacgaggag
      961 cgcagaccga tggccttgta gcgggtgacg atcgggtggg ccgggtcgac ccgcacgagg
     1021 gtgaggtcga aggagaacgc cgcctcgatg tcgacgagca ccgtcacctg ggtgccccgc
     1081 tgggacacgg tggtgatccc gtcggcgccg aggatgccct gcgacacgat gatctcggcc
     1141 ccgcgcaggg tcagccggtg ggtgtgcagc agatcggagg ccccaagcgc gatcgagccc
     1201 aacgcgacga ccgccccggc cgccggtgac agttcagtgg cgctcgcgct gaggggcgcg
     1261 aggatcgaca gggccccgag gatgtcgagg gccacctggt tggcgttgct gtcggtcatc
     1321 tcggccaagc cgtcgaggtc gccctccttg gctcggaact cgccgctgac ctcccacgcc
     1381 ccctgatcct cggcgatccg cagccggacg aggaaggtgc acacgccgtc cgccgcattg
     1441 cgtggcccgg agcgcagcgc cggttggccg cggcggcgca gcgacgcctc ggtcgccgtc
     1501 tcgatgtcga actcgccgta gatctcgcag cgcacgaacg tgctgcgcac gagctcgacg
     1561 cggacgccga tcttgtggaa gcagtccggt ggctgcgatc gggtcggcgt cgggtcggtg
     1621 cgggctggcg gggtgggcgc gggtcgggtc agctcgccgg tgaccgtgat cgaggcgggt
     1681 gggtcggcgc tgactgcccg cgcgcgccac caggacgcgg agcctgcggc cggcgcgggc
     1741 tggccatcga tcgatgtccc cgcccgagca gccgcggtgc cgtcggcgct gccggcgacg
     1801 acgttggtgt agcccaccga gtgcaaggcg gcgatcatgc cgtcgcgccg tcgggccgac
     1861 agggcccggt tgcgttgctc ctgagcgctg gtggagtccc cttcgtagga cgcaaaaccg
     1921 ctgaccgtca gctgcgtcgc ggcaccgatg accgacaggc gcgagcgtgc gtggtcgatg
     1981 aacagggcgc tcgccgaatg atgggtccgg tcggtcgcgg ggccgatgtg cgtgttggcg
     2041 gggttgacgg catacgcatc gccgtcggcg accttggggc ggtcgaagag gtagtaggcg
     2101 tcgagggtct gcacctgtcc cgccgtggtg acggcggtga ccgcaacggt cgccccctgc
     2161 gcgaccggca cctccgcctc ggtcccggtg atcgtgccgc cgggccaggc ccaggtcgcc
     2221 gccgcccctg acgagaggcg cacccgcgcg ctcgtcgggg tgctcggctc gacgagcacg
     2281 aggtatgccg cctcgctcga ggtcgtccgc acggtgaccg gagtgccgct cgcgcccgcc
     2341 gtcaccggcg tcgcgctgcg gctcacggtg atggtgcgcg tcgtgacgac gggtccaccg
     2401 ccgaccgcgg tgagggcgac ggcgaccgag atcgacccgg cgggcggcgt ggtgacgggc
     2461 gcgcggtcgt cgtcggtgcg gacgccgttg acggtgatcg acacggcgta ggtgccgagc
     2521 ccgccggacg actcggcata caccgtggtg tgctcgggga gttgcgc
//
LOCUS       DAOMZY010000423         2562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_40954_length_2562_cov_5.095333, whole genome shotgun sequence.
ACCESSION   DAOMZY010000423 DAOMZY010000000
VERSION     DAOMZY010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40954_length_2562_cov_5.095333"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..1433)
                     /locus_tag="PLK64_16435"
     CDS             complement(174..1433)
                     /locus_tag="PLK64_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HPV81381.1"
                     /translation="MSQPTYGAQDASASNAAAVQPLAPPAANDSLRLEAPPAAAAPIS
                     TTAAPRMAPAVPEAALPGLDAKVENYLQALMTAAPKSPQFETKANDVRVMGDEDIRRA
                     AESSNRLLRSPVKALQEGGLSEGGKVGKTLLELRRTVEDLDPSQATGVKKVLGMIPFG
                     DKVTDYFRKYQSAQSHLDGILQALRQGQDELQRDNVALNLEKQNLWDSMGRLNQYVYI
                     AERLDAKMAAQIATLDVTDPEKATALKQDVLFYVRQKHQDLLTQLAVSIQNYLAIDIT
                     IKNNIELVKGVDRASTTTVSALRTAVIVAQALGNQKLVLDQITALNTTTSGLIQSTSE
                     MLRDNSVRIQEQAASATIGIEQLQAAFSNIYQTMDAIDTFKVQALDNMAATIGTLETE
                     VAKSRTYLERANRVNPNVQMGSLDLGR"
     gene            complement(1631..2488)
                     /locus_tag="PLK64_16440"
     CDS             complement(1631..2488)
                     /locus_tag="PLK64_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPV81382.1"
                     /translation="MLWIAVPLGLLTGAVMGALGGGGAILTIPVLIYVVGMPPHDATT
                     ASLVIIGVSAAATAISHIRGGTARVPEAVILAAVGTIGTYAGSLASAAVAAPVLVVLL
                     ASLLAVVGALMLRQSSAVIEDLGSLIDGAAEGVADGADDGAVGGRHRPPPADDRAGGH
                     RVRRFGPVAAVGIGVGLLTGFFGVGGGFAIVPALTLVLGYPMRAAIGTSLLVIALNSA
                     TALGARVIQGVSLDWPLVGTFTLGAIVGGLIGGRLARRASPETLRRGFAMLLFLVAAY
                     MLGSAVAHR"
BASE COUNT          371 a          837 c          933 g          421 t
ORIGIN      
        1 gtcggcacac gcgggatcgg cgtgaccggc gcctgctcct cgcggccccc gaagagccat
       61 tcgccgatgc ccatggttgg tgattgtccc ctgtcccgtc tgctctgtct gtcgtggact
      121 tccctggccg agccctgacc cgaccctgac ccgcctggcc gcagcggtga gcgtcagcgg
      181 cccaggtcga gcgagcccat ctggacgttg gggttgaccc ggttggcgcg ctcgagatag
      241 gtccgcgact tggcgacctc ggtctcaagc gtgccgatcg tggccgccat gttgtccagg
      301 gcctgcacct tgaacgtgtc gatggcgtcc atggtctggt agatgttcga gaacgccgcc
      361 tgcaactgct cgatcccgat cgtggcgctg gccgcctgct cctggatgcg caccgagttg
      421 tcacgcaaca tctcggaggt cgactggatg agccccgagg tcgtcgtgtt gagggcggtg
      481 atctggtcga ggacgagctt ctggttgccg agggcctgcg ccacgatgac ggcggtgcgc
      541 agcgccgaca ccgtcgtcgt ggaggcccgg tcgacgccct tgacgagctc gatgttgttc
      601 ttgatggtga tgtcgatggc gaggtagttc tggatcgaca cggccaactg ggtgagcagg
      661 tcctggtgct tctggcggac atagaagagc acgtcctgct tgagggcggt ggccttctcg
      721 gggtcggtga cgtccaaggt ggcgatctgg gctgccatct tggcgtcgag gcgctcggcg
      781 atgtagacgt actggttgag ccggcccatc gagtcccaga ggttctgctt ttccaggttg
      841 agcgccacgt tgtcgcgctg caactcgtcc tggccctggc gtagggcctg caggatgccg
      901 tcgaggtggc tctgggccga ctggtacttg cggaagtagt cggtgacctt gtcgccgaat
      961 gggatcatcc cgaggacctt cttgacaccc gtcgcttggc tggggtcgag atcctcgacg
     1021 gtgcggcgca gttccagcag ggtcttgccg accttgccgc cctcggacaa tccgccctcc
     1081 tgcagcgcct tgaccggcga ccggagcagt cggttgctcg actcggcggc ccggcggatg
     1141 tcctcgtcgc ccatgacgcg cacgtcgttg gccttggtct cgaactgcgg cgacttcggg
     1201 gccgcggtca tcaacgcctg caagtagttc tcgaccttgg cgtccaggcc gggcagggcc
     1261 gcctcaggga cggccggggc catccgcggc gccgcggtag tcgagatggg tgcggcggcg
     1321 gccgggggag cctccagacg cagcgagtcg ttggccgcgg gcggtgccag cggctgcact
     1381 gccgcggcgt tgctggcgga ggcgtcctgc gcgccgtagg tcggctggct catctgtctc
     1441 cctgtcctgg agcgggtatg ccgtggtccg gctggtcgat cgggggtctt ggggcgtttc
     1501 ggtcaccgca gcaacaagag cggtgccgat agtccagacg tgcggccaat ctacgcggtt
     1561 ccccaggggt cgtgcagggg agctcggagc cgtcgcgcag gctttcggcc gggccactga
     1621 agcgcgatgg tcaccggtgc gcgacggcgg aaccgagcat gtatgccgcg acgaggaaca
     1681 agagcatcgc gaagccacgg cgcagtgtct cgggcgacgc gcggcgagct agtcgtccgc
     1741 cgatgagacc gccgacgatg gccccgagcg tgaaggtccc gacgagcggc caatccagcg
     1801 acacgccctg gatcactcgg gcgccgagtg cggtggcgct gttgagggcg atgaccagca
     1861 gcgacgtgcc gatggcggcg cgcatgggat agccgagaac gagcgtgagg gccgggacga
     1921 tggcgaaccc gccgccgacg ccgaagaagc cggtgagcag cccgacgccg atcccgaccg
     1981 cggccaccgg gccgaagcgc cgaacccggt gtccgccggc ccggtcgtca gcagggggcg
     2041 gtcgatgtcg gccgcccacc gccccgtcat cagccccgtc ggcgaccccc tcggccgccc
     2101 catcgatgag actgccgagg tcctcgatga cggcggagga ctggcgcaac atgagcgccc
     2161 ccacgacggc caacaggctg gccaggagga cgacgaggac cggcgcggcc accgcggctg
     2221 aggcgagcga cccggcatac gtgccgatcg tcccgacggc ggcgaggatc accgcctcgg
     2281 gcactcgggc ggtgccgccg cggatatggc tgatcgccgt ggcggccgcg ctcacgccga
     2341 tgatgaccag cgacgcggtc gtcgcatcgt gcggcggcat accgacgacg tagatgagga
     2401 cggggatggt gaggatcgcg ccgcccccgc cgagcgcccc catgaccgcg cccgtgagca
     2461 ggccgagtgg gacggcgatc cagagcaccg ggccactcta gagccaaccg atttctactg
     2521 tggccacgcg agcgtgtact ctctggaccg cactcctggg gc
//
LOCUS       DAOMZY010000424         2538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_41570_length_2538_cov_5.100685, whole genome shotgun sequence.
ACCESSION   DAOMZY010000424 DAOMZY010000000
VERSION     DAOMZY010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41570_length_2538_cov_5.100685"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PLK64_16445"
     CDS             <1..339
                     /locus_tag="PLK64_16445"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HPV81383.1"
                     /translation="HRTTHGHPFFDLDALVEGDVVIIETRQSYVVYRMARSTVVRPSQ
                     SEVVAPVPQKLGAKPTEAWLTLTTCHPKFSAAQRLIVFAKLDRVVPRSEGAPPEMTTA
                     PSALSLVGGA"
     gene            341..529
                     /locus_tag="PLK64_16450"
     CDS             341..529
                     /locus_tag="PLK64_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81384.1"
                     /translation="MYAALWRALPGRRGAKLATMILLFLIVVAVLFTWVFPAVAPLLP
                     FDQVTIDGAWAPKGVSGV"
     gene            492..1154
                     /locus_tag="PLK64_16455"
     CDS             492..1154
                     /locus_tag="PLK64_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HPV81385.1"
                     /translation="MEPGLRKACLVSERPTILVVDNYDSFVFTIVGYLEQLGAECTVV
                     RNDAVTPAEGAGYDGVLVSPGPGTPEDAGVSMAMIAACAERSQPMMGVCLGHQALGVV
                     MGATVGRAPELLHGKTSQVEHDGTGVLAGLPQPFTATRYHSLAIDPATLPAELLANGH
                     TASGLVMAVRHASLPLHGVQFHPESVLTEGGHRILANWLAMCGDSEAVARSAGLSPLV
                     HH"
     gene            complement(1248..>2538)
                     /locus_tag="PLK64_16460"
     CDS             complement(1248..>2538)
                     /locus_tag="PLK64_16460"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPV81386.1"
                     /translation="ARSDLYSTGCMLFELLTGRTPFVGETAVAIAYQHVGEPATPPSS
                     IRSAVPAAYDAITLHAMVKDRDARYQTALEFRDDLLAAGEGRAISAAARGTAANGTGG
                     FGVGAGGAAVGAVGDAAATTVLSEVGGPGGGSGMPPQDLRYDDDYDDEPLTRAGRRAS
                     QDPPKRRGAAYVLLTLAALAALALLFVLGRSILAQNAKPDQVAVPYLINQTEEQARAL
                     LSARKLLIDARNVTNDTVAKGLVVDQDPDSETMVSIGSTVTVQVSSGPGQAAIPSLAG
                     LTKDAAVTALKILNFPTPGVVVLDDPSQAKDAVIGTDPPAGTVVPLTQQITLKVASGK
                     VAVPSLVDQNQVTAQATLAGLKLLYAVAERVPTADTTILEGTVLSQDIVPGTLVDVGS
                     TVNVKIAIRLISTVTVTAPQPPPPPSTSSTATSTTGG"
BASE COUNT          347 a          899 c          869 g          423 t
ORIGIN      
        1 caccgcacca ctcacggaca cccgttcttc gaccttgatg ccctcgtcga aggtgacgtc
       61 gtcatcatcg agacccgcca gtcctacgtc gtctatcgga tggctcgctc gacggtcgtc
      121 cggccgagcc agtccgaggt cgttgctccg gtgccgcaga agctgggcgc caagcccacc
      181 gaggcctggc tgacgctgac cacgtgccac ccgaagttct ccgcggccca gcgcctcatc
      241 gtcttcgcca agctcgaccg ggtcgtcccc cgcagcgaag gcgccccgcc ggagatgacg
      301 accgccccgt ccgccctctc gctcgtcgga ggtgcctgag atgtatgccg ccctctggcg
      361 agcgctcccc ggcaggcgcg gggccaagct ggccacgatg atcctgctct tcctcatcgt
      421 cgtcgccgtg ctcttcacct gggtcttccc cgcggtcgcg cccctgttgc ccttcgacca
      481 ggtgaccatc gatggagcct gggctccgaa aggcgtgtct ggtgtctgag cgccccacga
      541 tcctcgttgt cgacaactac gactcgttcg tcttcaccat cgtcggctac ctcgagcaac
      601 tcggcgccga gtgcacggtg gtccgcaacg acgcggtgac ccctgccgaa ggcgccggct
      661 acgacggggt gctcgtctca ccgggccccg gcacccctga ggacgccggc gtgtcgatgg
      721 cgatgatcgc ggcctgcgcc gagcgctcgc agccgatgat gggcgtctgc ctgggccatc
      781 aggcactcgg cgtggtcatg ggggcgacag tcggccgggc tccagagttg ctgcatggca
      841 agacctcgca ggtcgagcat gacggcacgg gcgtcctcgc cgggctgccg cagccgttca
      901 ccgccacccg ctatcactcg ctggcgatcg atccggccac cctcccggcc gagctactag
      961 ccaacggcca caccgccagc ggcctggtca tggcagttcg ccacgccagc cttccgctgc
     1021 acggcgtgca gttccacccc gagagcgtgc tcaccgaggg cggccaccgg atcctcgcca
     1081 actggttggc gatgtgtggt gactccgagg ccgtcgccag atcggccggc ctcagcccgc
     1141 tcgtccacca ctgacgcccg ccactgacgt ccgccggcgg gccgtcagcc actggcgtcc
     1201 gccggccggc tgtcagcctc cgcgtacgct ccggccccta cctccggcta ccctcccgtg
     1261 gtcgacgtcg ccgtcgatga ggtggacggc gggggcggcg gttggggtgc tgtgaccgtg
     1321 accgtcgaga tgagccggat ggcgatcttg acattgaccg tcgagccgac gtcgaccaac
     1381 gttccgggga cgatgtcctg gctgagcacc gtgccctcaa ggatcgtcgt gtctgctgtc
     1441 ggcacccgct cggcgaccgc atagaggagt ttgagtccgg cgagcgtggc ctgggccgtc
     1501 acctggttct ggtcgacgag gctgggcacc gcgaccttgc ccgaggccac cttgagggtg
     1561 atctgttggg tcaacgggac gaccgtgccg gccggcggat cggtgccgat gacggcgtcc
     1621 ttggcctgcg acgggtcgtc gagcacgacg acgccgggtg ttgggaagtt gaggatcttc
     1681 agggcggtga ctgccgcgtc tttggtcaac ccagcgagcg aggggatcgc cgcctgacct
     1741 ggcccggacg acacttggac ggtcaccgtg ctcccgatgc tgaccatggt ctcggagtcg
     1801 gggtcctggt cgacgacgag tcccttggcg actgtgtcgt tggtgacgtt acgggcgtcg
     1861 atgaggagtt tgcgcgccga cagcaatgcc cgggcttgct cctcggtctg gttgatgagg
     1921 tagggcactg ccacctggtc cggcttggcg ttctgggcca ggatgctgcg gccgaggacg
     1981 aacagcaggg ccagagcggc cagtgccgcg agggtgagca gcacgtatgc cgccccgcgt
     2041 cgcttgggcg ggtcctgcga cgcccgacgt cccgcgcgag tgaggggctc gtcgtcatag
     2101 tcgtcgtcgt aacggaggtc ctgcggcggc ataccggatc cgccgcctgg accacccacc
     2161 tcggagagga ccgtcgtggc ggccgcgtct ccgaccgcgc cgaccgctgc gccccccgcg
     2221 cccacgccga acccacccgt gccgttcgca gcggtgccgc gggcggcggc actgattgcc
     2281 cggccctccc ccgcggcgag caggtcgtcg cggaactcca acgcggtctg gtagcgcgcg
     2341 tcgcggtcct tcaccatggc atgcaaggtg atcgcgtcgt atgccgcggg caccgccgag
     2401 cggatcgacg acggcggcgt ggctggctcg ccgacgtgct ggtaggcgat cgcgaccgca
     2461 gtctcaccga cgaacggcgt gcgcccggtg agcagttcga agagcatgca cccggtcgag
     2521 tagaggtcgg agcgggcg
//
LOCUS       DAOMZY010000425         2488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_42852_length_2488_cov_5.716400, whole genome shotgun sequence.
ACCESSION   DAOMZY010000425 DAOMZY010000000
VERSION     DAOMZY010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42852_length_2488_cov_5.716400"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="PLK64_16465"
     CDS             complement(<1..958)
                     /locus_tag="PLK64_16465"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HPV81387.1"
                     /translation="MTVIRRKPKRHLMVFSGRAHPALADAVAADLGTKVVPTQAYDFA
                     NGEIYVRYEESVRGCDAFVIQSHTVPINEWIMEHLIMVDALKRASAKRITVVLPFYGY
                     ARQDKKHRGREPISARLMADLFRTAGADRLMAVDLHTAQIQGFFDGPVDHLMALPMLA
                     DYVIANYGKKKLAVVSPDAGRIKVAEQWSARLGGAPLAFVHKTRDITRPNESVANRVV
                     GDVAGRMCILVDDMIDTGGTITKAADAIMAEGAAGVIVAATHAILSGPAVDRLRASSA
                     REVIVTDTLPIPIDRQFDKLTVLPIAPLISRAIMEVFEDGSVT"
     gene            complement(955..2433)
                     /gene="glmU"
                     /locus_tag="PLK64_16470"
     CDS             complement(955..2433)
                     /gene="glmU"
                     /locus_tag="PLK64_16470"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HPV81388.1"
                     /translation="MTPPRPTAVIVLAAGEGTRMKSAMPKVLHTIGGRSLVHHAIAAA
                     RGIDPIHLEVVVRHERDLVAAHIAECDPDCLIADQDEIKGTGRAVECGLAALPAELTG
                     TIVVTYGDVPLLTSETLREVLAAHEGASVAVTVVTSHIPDPTGYGRIVRDADGSVARI
                     VEQKDATDDERAITEVNSGIYAFEATLLTQALAGLGTDNAQGEKYLTDVVGIARAQGR
                     AVAAYVLADVWQTEGVNDKIQLARLGAELNRRTVEAAMREGAIIRDPATTWIDTDVSI
                     GRDTVIEPNVQLRGATTIGADCLIGPDTTLVDMEVGDGATVCRTQGSLSVVGPGATVG
                     PWAYLRPGTELGAEAKIGTFVETKNANIGAGSKIPHLSYVGDATIGTGTNIGASSVFV
                     NFDGVAKHRTTVGDHCRMGSDTMYVAPVTIGDGAYSGAGTVIRSDVPPGALVLTDAPQ
                     RVIEGWVAEHRPDTEAARAAAAAGAAAASERHTGDNEQETQR"
BASE COUNT          353 a          835 c          882 g          418 t
ORIGIN      
        1 tggtgaccga cccgtcctcg aacacctcca tgatcgcccg cgagatgagc ggggcaatcg
       61 gcagcacggt gagcttgtcg aactgccggt cgatcgggat cggcagggtg tccgtgacga
      121 tgacctcgcg agcgctcgaa gcccgcagtc ggtcgactgc gggacccgag aggatcgcgt
      181 gggtcgcggc cacgatgacg cccgcagcgc cctcggccat gatcgcgtcg gccgccttcg
      241 tgatggtgcc gccggtgtcg atcatgtcgt cgacgaggat gcacatccgc ccggcgacgt
      301 caccgacgac ccggttggcg acgctctcgt tggggcgggt gatgtcgcgg gtcttgtgga
      361 cgaaggccag cggtgcgccg ccgagacggg ccgaccactg ctcggcgacc ttgatccggc
      421 cggcgtccgg cgagacgacc gcgagcttct tcttgccgta gttggcgatg acgtagtcgg
      481 cgagcatcgg caaggccatg aggtggtcga ccggcccgtc gaagaagccc tgaatctgag
      541 ccgtgtgcag gtcgacggcc atgagccggt cggcccccgc ggtgcggaac aggtcggcca
      601 tcagccgcgc cgagatcggc tcgcggccgc ggtgcttctt gtcctggcgg gcatagccat
      661 agaacggcag cacgacggtg atccgcttgg ccgaggcgcg cttgagcgcg tcgaccatga
      721 tgaggtgctc catgatccac tcgttgatgg gcaccgtgtg gctctggatg acgaacgcgt
      781 cgcagccgcg caccgactcc tcgtagcgca cgtagatctc gccgttggcg aagtcatagg
      841 cctgcgtcgg cacgactttc gtccccaggt cagcggccac cgcatcggcc agcgccgggt
      901 gggcgcgccc agagaacacc atgaggtgcc gcttgggctt cctccggatc accgtcaccg
      961 ttgcgtctcc tgctcgttgt ctccggtatg ccgttcactc gccgctgctg cccctgctgc
     1021 tgccgccgcg cgcgcagcct ccgtgtcggg ccgatgctca gccacccacc cctcgatcac
     1081 gcgctgcggg gcatcggtca acacgagagc accaggcgga acgtcactgc ggatgacggt
     1141 cccggctccg gagtaggccc cgtcgccgat cgtcacgggc gcgacataca tcgtgtcgct
     1201 gcccatccgg cagtggtcgc cgaccgtggt gcggtgcttg gccactccgt cgaagttgac
     1261 gaagaccgac gaggcaccga tattcgtccc ggtgccgatc gttgcgtcgc cgacgtagga
     1321 caggtgcgga atctttgacc ccgcaccgat attcgcgttc ttggtctcga cgaaggtgcc
     1381 gatcttggcc tcggcgccga gctcggtgcc cggccgcagg taggcccacg ggccgaccgt
     1441 ggcacccggg ccgacgaccg acagggagcc ttgcgtgcgg cataccgtcg cgccgtcccc
     1501 gacctccatg tcgaccagcg tcgtgtcggg cccgatgagg cagtccgcgc cgatggtggt
     1561 ggcgccccgc aactggacgt tgggctcgat gacggtgtcg cgcccgatcg agacgtcggt
     1621 gtcgatccac gtcgtcgccg ggtcgcggat gattgcgccc tcgcgcatcg cggcctcgac
     1681 ggtgcgccgg ttgagctccg cgccgaggcg ggcgagttgg atcttgtcgt tgacaccctc
     1741 ggtctgccac acgtcagcga ggacgtatgc cgccacggcc cgcccctgcg cgcgggcgat
     1801 gccgacgaca tcggtgaggt acttctcgcc ttgggcgttg tcggtgccca gaccggccaa
     1861 ggcctgggtg agcagggtgg cctcgaaggc gtagatgccc gagttgacct cggtgatcgc
     1921 ccgctcgtcg tcggtggcgt ccttctgctc gacgatccgg gccacgctgc cgtcggcgtc
     1981 gcggacgatc cggccatacc cggtcgggtc cgggatgtgc gaggtgacga ccgtgactgc
     2041 gacggaggct ccctcatggg cggcgagcac ctcgcgcagg gtctcggagg tgagcagtgg
     2101 gacgtcgccg taggtgacga cgatggtgcc ggtcagctcg gcgggaaggg cagccaggcc
     2161 gcactcgacg gcgcggccgg tgcccttgat ctcgtcctga tcggcgatga ggcagtcggg
     2221 gtcgcactcg gcgatgtggg cagcgacgag gtcacgttcg tgccgcacga cgacctcaag
     2281 gtggatgggg tcgatgcctc tggctgccgc gatcgcatgg tggaccaggc tgcgcccacc
     2341 gatggtgtgc aggaccttcg gcatcgccga cttcatccgg gtgccctcgc ccgcggcgag
     2401 gacgatgact gctgtcgggc gcggtggcgt cacgtcgaac gatcctaccg gtcgccccga
     2461 ctacccggcg cgccgacgag aaccgcga
//
LOCUS       DAOMZY010000426         2481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_43041_length_2481_cov_5.928277, whole genome shotgun sequence.
ACCESSION   DAOMZY010000426 DAOMZY010000000
VERSION     DAOMZY010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43041_length_2481_cov_5.928277"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PLK64_16475"
     CDS             <1..225
                     /locus_tag="PLK64_16475"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPV81389.1"
                     /translation="IPSIVATYPATTWHERETFDMFGIIFDGHPALTRILMPDDWPGH
                     PQRKDYPLGGIPVEYKGATVPPADERRAFN"
     gene            249..1601
                     /locus_tag="PLK64_16480"
     CDS             249..1601
                     /locus_tag="PLK64_16480"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HPV81390.1"
                     /translation="MAQQSAEHTATGGAEEFLDSPAFTVSGSGDWDDLVDEATSLHEE
                     RIVVNMGPQHPSTHGVLRLILELDGETVTETRAGIGYLHTGIEKNMEYRTWVQGVTFC
                     TRMDYLTPMFQETAYCLAIEKLLGITDAIPERASVIRVLMMELTRISSHLVCIGTGGM
                     EMGATTVMTIGFRERERIFQIIEMITGLRMNNAYIRPGGVAQDLPPGAIDKIRELVPE
                     IRQGIRELELLVNENPIVKGRMVGVGHLDLAGCMALGITGPMLRSASLPHDLRKATPY
                     CGYETYDFDVITRDSADSYGRLRVRLDEMYQSLRIVLQCADRLEVSVGDPVMVGDKKV
                     AWPAQLAIGTDGMGNSLDHIRTIMGESMEDLIHHFKLVTEGFRVPAGQVYVAVESAKG
                     ELGCHLVSDGGTRPYRAHFRDPSFNNLQATSIMCEGGQVADVIVAVASIDPVMGGVDR
                     "
     gene            1598..2362
                     /gene="nuoE"
                     /locus_tag="PLK64_16485"
     CDS             1598..2362
                     /gene="nuoE"
                     /locus_tag="PLK64_16485"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPV81391.1"
                     /translation="MSGHDASGHLHVSAESKAPYAADVLATLRQDAAAVIARYPVKRS
                     ALLPLLHLVQSVDGYVTGRGIELCAELLDLSTAEVSGVATFYTQYKRHPNGDYTLGVC
                     TNTLCAIMGGDAIWDAVSEHLGVGHDETTADGKVTLERIECNAACDYAPVVMTNWEFF
                     DNQTPDSVIRLADDLRAGKDVRPTRGPDKVCTFKEVSRVLAGFSDGRADEGPAAGPAS
                     LLGLKIAHEHGWAAPQPPAADPAPTAPAAPTKGASK"
BASE COUNT          429 a          894 c          789 g          369 t
ORIGIN      
        1 atcccgtcga tcgtcgcgac ctacccggcg acgacgtggc acgagcgcga gaccttcgac
       61 atgttcggga tcatcttcga cggtcacccg gcgctcaccc ggatcctcat gcccgacgac
      121 tggcccggtc acccgcagcg caaggactat ccgctgggtg gcattccggt cgaatacaag
      181 ggcgcgaccg tgcccccggc cgacgagcgg agggcgttca actgatggcc gccgacctgg
      241 gacctgacat ggcacagcag agtgcagagc acaccgcgac cggcggcgcc gaggagttcc
      301 tcgactcccc ggccttcacc gtgagcggct ccggcgactg ggacgacctc gtcgacgagg
      361 ccacctcgct gcatgaggag cggatcgtcg tcaacatggg tccgcagcac ccgtccaccc
      421 acggcgtcct gcggctcatc ctcgagctcg acggcgagac ggtgaccgag acccgcgcgg
      481 gaatcggcta cttgcacacc ggtatcgaga agaacatgga ataccggacg tgggtccagg
      541 gggtgacctt ctgcacccgg atggactacc tcaccccgat gttccaggag acggcctact
      601 gcctggccat cgagaagctc ctcggcatca ccgacgccat ccctgagcgg gcctcagtca
      661 tccgggtcct catgatggag ctcacccgga tctcctcgca cctggtgtgt atcggcaccg
      721 gtggcatgga gatgggcgcc accacggtca tgacgatcgg tttccgtgag cgcgagcgga
      781 tcttccagat catcgagatg atcaccgggt tgcggatgaa caacgcctac atccgccccg
      841 gtggtgtcgc ccaggacctg cctccgggcg cgatcgacaa gatccgcgag ttggtgcccg
      901 agatccgcca gggcatccgc gagttggagt tgctcgtcaa cgagaacccc atcgtcaagg
      961 gccgcatggt cggcgtcggt cacctcgacc tggccggctg catggccctc ggcatcaccg
     1021 gcccgatgct ccgctcggcg agcctgccgc acgacctgcg caaggcgacc ccctattgcg
     1081 gttacgagac ctacgacttc gacgtcatca ctcgcgacag cgccgactcc tacggccggt
     1141 tgcgggtccg cctcgacgag atgtaccagt cgttgcggat cgtcctgcag tgcgccgacc
     1201 ggcttgaggt cagcgtcggt gacccggtca tggtcggtga caagaaggtc gcctggcccg
     1261 cccagttggc catcggcacc gacggcatgg gcaacagcct cgaccacatc cgcacgatca
     1321 tgggcgagtc gatggaagac ctcatccatc acttcaagct cgtcaccgag ggcttccggg
     1381 tcccggccgg tcaggtctac gtcgcggtcg agagcgccaa gggcgagctc ggctgccacc
     1441 tggtctccga cggcggcacc cgcccctatc gcgcgcactt ccgcgatccc tcgttcaaca
     1501 acctgcaagc gacgtcgatc atgtgcgaag gcggccaggt cgccgacgtc atcgtcgccg
     1561 tcgcatcgat cgaccccgtc atgggaggtg tggaccggtg agcggccacg acgcatcagg
     1621 ccacctgcac gtcagcgcgg agagcaaggc gccgtatgcc gcggacgtcc tggccaccct
     1681 ccggcaggac gccgccgcgg tgatcgcccg atacccggtc aagcgttcgg cgctgctgcc
     1741 gctgctgcac ctcgtgcagt cagtcgacgg ctacgtcacc ggccgaggca tcgagctctg
     1801 cgccgagttg ctcgacctga gcaccgccga ggtgagcggg gtcgcgacct tctacacgca
     1861 atacaagcgc caccccaacg gcgactacac gctcggcgtc tgcaccaaca cgctctgcgc
     1921 gatcatgggc ggcgacgcca tctgggacgc cgtgtccgag cacctcgggg tgggccacga
     1981 cgagaccacg gccgacggca aggtcaccct tgagcgcatc gagtgcaacg cggcgtgcga
     2041 ctacgccccg gtcgtcatga ccaactggga gttcttcgac aaccagacgc ccgactcggt
     2101 gatccgcctc gccgacgacc tgcgggccgg caaggacgtc cgccccaccc gcggtcccga
     2161 caaggtgtgc accttcaaag aggtgtcccg cgtgctggcg ggcttcagcg atggccgcgc
     2221 agacgagggc ccggccgccg ggcccgcgtc gttgctcggc ttgaagatcg cccacgaaca
     2281 cggctgggcc gcgccgcagc cgccggccgc cgacccggca ccgaccgcac cggcagcacc
     2341 gaccaagggg gcctcgaagt gaccgacacc ctcaccccgg tcctcacggc ccactgggac
     2401 cagcagcgtt cctggacgct cgcggcatac gagcaaacgg gtgggtatgc cgccctccgc
     2461 aaggcgatga gcatgccggc c
//
LOCUS       DAOMZY010000427         2454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_43784_length_2454_cov_6.272197, whole genome shotgun sequence.
ACCESSION   DAOMZY010000427 DAOMZY010000000
VERSION     DAOMZY010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43784_length_2454_cov_6.272197"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="PLK64_16490"
     CDS             complement(<1..1004)
                     /locus_tag="PLK64_16490"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPV81392.1"
                     /translation="MTTHLPRPTPTRRAVLSMLGLGVLGLAGCAASSGTDSTATSSGS
                     SGTSSTGASAAGASAGRGTGVFAADVIHTVTAAAEASLFTGALATFKSSGTKEWIDVE
                     VTLDGTTFQHVGMRLKGNSSLRSVAAGTAPQLLPWLVKLDKFVKGQAHEGVSEFVVRS
                     NSSATALNEAVALDLLAASGLSAQRAAYIALSVNDSAPALRLIVENPGDEWVDRVFNA
                     DGKLYKAEATGDYTYRGSDPAAYDEVFDQEAGDKDLTPLMGFLQFVNDSSDADFVAGL
                     ADRLDTAAFARYLAFETLVDNFDDIDGPGNNSYLWWDADTKKMTVVAWDHNLAFGLRP
                     "
     gene            complement(1204..1641)
                     /locus_tag="PLK64_16495"
     CDS             complement(1204..1641)
                     /locus_tag="PLK64_16495"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HPV81393.1"
                     /translation="MDPALLDLTAGACRRLIEERFPDDDHHGAAAMLLRDGTILTGTS
                     PNAINPSVEVCHEIEPYCAAYRLDQSVVASVCLHRQPGGRFVVLSPCGVCRERLAVHG
                     PAVLVAVADPNDPSVIVWKTLKDVLPDYWMTAFPDEVDPAWSA"
     gene            complement(1764..2231)
                     /locus_tag="PLK64_16500"
     CDS             complement(1764..2231)
                     /locus_tag="PLK64_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HPV81394.1"
                     /translation="MSQVVDFVTVSTAGLESSPHAAALAGLRANESRYFKNKYAHDFT
                     VTPADDAPEVVAWVATILQERNIAIAARPLEVSVADVDGIHWVHVFYESGLAINVLYG
                     VEAGAKRAVGFKLSEGMEVPEELSGFKFARQKSKLAGIMRGSFFVIKGEYDPS"
     gene            complement(2262..>2454)
                     /locus_tag="PLK64_16505"
     CDS             complement(2262..>2454)
                     /locus_tag="PLK64_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPV81395.1"
                     /translation="VDMMAKGTFGAINFATPSLDTTFAELEAAGVDIVQEPLDQPWGL
                     RDCAVRDPAGNLVRIIQVR"
BASE COUNT          411 a          830 c          821 g          392 t
ORIGIN      
        1 ttcggccgga gcccgaacgc cagattgtgg tcccaggcca cgacggtcat cttcttcgta
       61 tcggcgtccc accacagata cgagttgttg cccggcccgt cgatgtcatc gaagttgtcg
      121 accagcgtct cgaacgccag gtacctcgcg aaggccgccg tgtccaaccg atcagccaga
      181 cccgcgacga agtcggcgtc gctggagtcg ttgacgaact gcaggaaccc catcagcggg
      241 gtcaggtcct tgtcgccggc ctcctggtcg aacacctcgt cgtatgccgc ggggtcgctt
      301 ccccggtagg tgtagtcgcc ggtcgcctca gccttgtaga gcttgccgtc ggcgttgaac
      361 acccggtcca cccactcgtc accggggttc tccacaatca gccgtagcgc gggcgccgag
      421 tcgttgacgc tcaaggcgat gtatgccgcg cgctgggccg acaggccact agcggcgagc
      481 aggtcgagcg ccaccgcttc gttgagtgcc gtcgcggaac tgttggagcg cacgacgaac
      541 tcgctcaccc cctcgtgagc ctggcccttg acgaacttgt cgagcttgac cagccacggc
      601 agcagttgcg gtgcggtgcc cgcggcgacc gagcgcagcg aggagttgcc cttcaatcgc
      661 atccccacgt gctggaacgt cgtgccgtcc agggtcacct cgacatcgat ccactccttg
      721 gtgccgctgc tcttgaaggt cgccaacgcc ccggtgaaga gcgatgcctc agccgcagcc
      781 gtgaccgtgt ggatgacgtc ggccgcgaag acccccgtgc cacgccctgc cgaggcaccc
      841 gccgccgacg ctccggtcga tgaggtgccg gacgaaccgc tcgaggtcgc ggtcgaatcg
      901 gtgccggagc tggcggcaca cccggccagg ccgaggacgc ccagtccgag catcgagagc
      961 acggcccgtc gggtcggggt gggccggggc aggtgcgttg tcatcgccca aagacaccct
     1021 gggagcctgt gaaccccctg tgcgcagagc ctccgtttca cgtgaagcag gcgaacggca
     1081 tacagacgaa tgcggcgaca accgggcgag aagccgccct tcaatcgcga gctgcccgag
     1141 ttgtcgaagc agggccatgg gccagcacaa gcgcggcggg gcgcgggggg cactagcgcc
     1201 ggctcaggcg ctccacgcgg ggtcgacctc gtcggggaac gctgtcatcc agtagtccgg
     1261 cagcacgtcc ttgagcgttt tccacacgat gaccgacgga tcgttcggat ccgcgaccgc
     1321 gaccagaacc gccgggccgt gcaccgccag gcgctcacgg cataccccgc agggactgag
     1381 caccacgaac ctgcccccgg gctggcggtg caagcagacc gacgcgacca cagattgatc
     1441 gaggcggtat gccgcgcagt agggctcgat ctcgtggcag acctcgacgg acgggttgat
     1501 ggcgttcggg gaggttcccg tgaggatcgt tccgtcgcgg agcagcatgg cggctgcacc
     1561 gtggtggtcg tcgtccggga atcgctcctc gatcaaccgt cggcaggcgc cggctgtcag
     1621 gtcaaggagt gcggggtcca tgccgtgacc atagggcgcg tcgatgcggc tgcgggcggg
     1681 aagccgctca tgaagccgcg accggtggcg tgatcacgca cgctacggcg acttcatggg
     1741 cgacttcatc ccctgacccg cactcagctg gggtcgtact cacctttgat gacgaagaag
     1801 gagccgcgca tgatgccggc gagcttggac ttctgccggg cgaacttgaa ccccgacaac
     1861 tcctcgggca cctccatccc ctcggacagt ttgaaaccga ccgcccgctt ggcccccgcc
     1921 tcgacgccgt agagcacgtt gatcgccagg cccgactcgt agaacacgtg cacccagtgg
     1981 atgccgtcga cgtcggcgac cgacacctcc aacgggcgcg cggcgatcgc gatgttgcgc
     2041 tcctgcagga tcgtcgcgac ccacgcgacg acctccgggg catcgtcggc cggcgtgacg
     2101 gtgaagtcgt gggcgtactt gttcttgaag tagcgcgact cgttcgcccg caggcccgca
     2161 agcgcggcgg cgtgcggcga ggactccagg cctgccgtgg acacggtgac aaagtcgacg
     2221 acttggctca tgcgatctcc tgacgagtgg gtgaaaaagc ttcagcggac ctggatgatg
     2281 cgcacgaggt tgccggcggg atctcgcacg gcgcagtcgc gcagccccca cggctggtcg
     2341 agcggctcct ggacgatgtc cacgcccgcc gcttcgagct cggcgaacgt cgtgtcgagc
     2401 gacggggtcg cgaagttgat cgccccgaac gttcccttgg ccatcatgtc gacg
//
LOCUS       DAOMZY010000428         2419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_44729_length_2419_cov_5.843909, whole genome shotgun sequence.
ACCESSION   DAOMZY010000428 DAOMZY010000000
VERSION     DAOMZY010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44729_length_2419_cov_5.843909"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..1924
                     /locus_tag="PLK64_16510"
     CDS             218..1924
                     /locus_tag="PLK64_16510"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HPV81396.1"
                     /translation="MYLRTTKRRNADGSEVRYYQLAENVWDAARGCAVAKVVYNFGRA
                     DQVDSEMLKRLAASILRVTGDGGAAARVIAPDGSAPVQVRDAWPYGGVYVLGQLWREV
                     GIDRVLREHARSAKTRDAFERALFAMVANRALSPYSKLYCWEQWLREEVFLPSATDLE
                     LHHLYFAMDFLEDHKTAIEKAVYFQMADLLSVDVDLIFYDTTSLHFEVDEEDETEQHH
                     HGRTYDASRKRGHAKNGRGDAPQIVIGLAVTRDGLPVRSWVFPGNRADVSTVAKVKED
                     LRGWRLGRCVFVGDAGMNSADNRRSLALGGGKYILATRMRGNDEVTNEVLSRAGRYQE
                     VRDNLRVKEVIVSDGERRSRYVVCHNPAEAERQRQHRERTLAELEVELASLRQCNAGV
                     HSKRVCELRTSARYGKYLKESATGQLSVDRGAVADAARYDGKWVITSNDDTLSAEDLA
                     LGYKQLMRVEECWRTLKSGLRLRPVFHRAPWRIQAHVTISVLSLLLERIAELRAADTW
                     RNVRAQLERIQVVEYDHAQARIRQTTELRPEADALLRKLRVAAPPKLHAIEHPPVLDA
                     DA"
     gene            complement(1985..2227)
                     /locus_tag="PLK64_16515"
     CDS             complement(1985..2227)
                     /locus_tag="PLK64_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81397.1"
                     /translation="MDDEPNFKLSVYESLVGDSSVQGLVWKVLKEAEMDGSVRVSPMD
                     VAAARRYLRRCLLNMSMGELASLQPFDPTLKTGHRV"
BASE COUNT          436 a          754 c          828 g          401 t
ORIGIN      
        1 cgttcgctgc aaggcccgag gcagggagcg ggctccacgt tccctccccc ggatcgaacg
       61 ccgccccatc gcccgacttt gaagattttt cgacactttg ggcacctggc gctagcgatt
      121 tcgggcagtt acgcgccgcc gagcccccca gagcataggc acacgacctg ttggcgaagc
      181 cttgcaatgc gtgtgcccag gcatgtattg gctaggcatg tacctgcgca cgacgaagcg
      241 gcgtaacgcg gacggctcgg aggtccggta ctaccaactg gccgagaacg tatgggacgc
      301 ggcgcgtggc tgcgcggtgg ccaaggtcgt ctacaacttc ggtcgagccg atcaggtcga
      361 cagcgagatg ctgaagcggc tggcggcgag catcctgcgg gtgacgggag acggaggggc
      421 cgccgcgcgg gtgatcgcgc cggatggtag cgccccggta caggtgcggg atgcctggcc
      481 gtacggcggg gtatacgtgc tggggcagtt gtggcgggag gtggggatcg accgggtgct
      541 gcgcgagcac gcgcggtcgg cgaagacgcg ggacgccttc gagcgggcgc tgttcgccat
      601 ggtcgcgaac cgggcgctgt cgccgtactc gaagctgtac tgctgggagc agtggctgcg
      661 cgaagaggtg ttccttccga gcgcaacgga cctggagttg catcacctgt acttcgcgat
      721 ggacttcctc gaagaccaca agacggccat cgagaaggcg gtctacttcc agatggctga
      781 cctgctcagc gtcgacgtcg acttgatctt ctacgacacc acgtcgctgc acttcgaggt
      841 cgacgaggaa gatgaaaccg agcagcacca ccacggtcgg acctacgacg ccagccgcaa
      901 gcgcgggcac gcgaagaacg ggcggggaga cgcaccgcag atcgtgatcg ggctggcggt
      961 cacgcgggac gggcttcccg tgcgctcttg ggtcttcccg gggaaccggg ctgacgtgtc
     1021 gacggtcgcg aaggtgaagg aggacctgcg gggatggcga ctcggacgct gcgtgttcgt
     1081 cggcgacgcc gggatgaact cggcggacaa ccgccggagt ctggcgctgg gtgggggcaa
     1141 gtacatcctc gccacgcgca tgcggggcaa cgacgaggtg acgaacgagg tcctgtccag
     1201 agccggtcgc taccaggagg tccgcgataa cctccgcgtc aaggaggtca tcgttagcga
     1261 cggcgagcgc cggagtcgtt acgtcgtctg ccacaaccct gcggaggcgg agcggcagcg
     1321 ccagcatcgc gagcgaactc tggccgaact ggaggtcgag ctcgcgtccc tgcggcagtg
     1381 caatgcggga gtacacagca agcgggtgtg cgagttgcga acgagcgcgc gctacgggaa
     1441 gtacctgaag gagtccgcca ccggccagct cagcgttgac cgtggcgccg tcgctgacgc
     1501 cgcccgctac gacgggaaat gggtcatcac gtcgaacgac gacaccctgt ccgctgagga
     1561 tctggccctt ggctacaagc agttgatgcg ggtcgaggag tgctggcgca cgctcaagtc
     1621 ggggctccgc ttgcgtccgg tcttccaccg ggcaccgtgg cggatccagg cccacgtgac
     1681 catctccgtc ttgtcgttgc tgctcgagcg gatcgccgag ctgcgtgcag ccgacacctg
     1741 gcggaacgtg cgggctcagc tcgagcgtat ccaagtggtt gagtacgatc acgcccaggc
     1801 gcgtatccgg cagacgaccg aactccgacc cgaagctgac gctctgctcc gcaaactccg
     1861 ggtcgccgcc ccgcccaagt tgcacgccat cgagcatccg ccggtgctgg acgccgacgc
     1921 ctagtaacac gaccgcaatc ggtggatctg tctcaagcca aggagatcat tgagctatgt
     1981 ctacctagac tcggtgaccg gttttcaaag tcgggtcgaa cggctggagc gaggcgagct
     2041 cgcccatgct catgttgagg agacagcggc ggaggtagcg ccgtgcagcg gccacgtcca
     2101 tggggctcac ccggacggag ccgtccatct cggcctcctt cagcaccttc cacaccaagc
     2161 cctgcacgga gctgtccccg accagggact cgtacacgga cagcttgaag ttcggttcgt
     2221 cgtccatggt cagcaagata gggtctgcat ggcagagtgc acgatcgtcg acgccgcctt
     2281 ggcgcgcggt cacacatatc ctcaattata attactactc agcgaggatc gcgcgcgcag
     2341 ctgccgcaat cgcgcggatc gtcgcgttgt gcctctgccc atatccaatc acagatcgcc
     2401 tgcggttccg tggcatctg
//
LOCUS       DAOMZY010000429         2414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_44883_length_2414_cov_5.423485, whole genome shotgun sequence.
ACCESSION   DAOMZY010000429 DAOMZY010000000
VERSION     DAOMZY010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44883_length_2414_cov_5.423485"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /gene="rnc"
                     /locus_tag="PLK64_16520"
     CDS             complement(<1..696)
                     /gene="rnc"
                     /locus_tag="PLK64_16520"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPV81398.1"
                     /translation="MGQTGGGPAPSLTTLRDYLNGVIGTPVDDEVLERALTHRSYAYE
                     NGGLPNNERLEFLGDAVLGLVITDTLYAAHPDLPEGQLAKLRASVVNMRALADVGRTI
                     DVGGYVRLGRGEETTGGRDKASILADTMEAIIGTIYLSGGLRAAAEFVHHLFDPLLAT
                     AAGLGAGLDWKTSLQELCSAGSLGAPDYRLVESGPDHEKSFVAAVLVAHEVLGEGAGR
                     SKKEAEQAAAEQAW"
     gene            complement(709..903)
                     /gene="rpmF"
                     /locus_tag="PLK64_16525"
     CDS             complement(709..903)
                     /gene="rpmF"
                     /locus_tag="PLK64_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPV81399.1"
                     /translation="MAVPKRKTSRSNTRSRRANWKATAAVLTTCPRCSQPTPPHQACP
                     SCGTYKGRHVAAAERSEHQG"
     gene            complement(905..1480)
                     /locus_tag="PLK64_16530"
     CDS             complement(905..1480)
                     /locus_tag="PLK64_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HPV81400.1"
                     /translation="MSRLDSRAPLVFDTRELRRQPGSMQEYTRQVEVPEEMGTDIVAV
                     PAGSPVDIELRLESVSEGVLVSGSVQATATGACVRCLDPVSLPVEVSFQELFVYADRA
                     AHHHEVDADSDEAEVYELVDDLVDLQPVLRDAVVPALPFQPVCRVDCPGLCSECGVAL
                     ALDPDHHHDVLDPRWAALGTMLSDDPEENRT"
     gene            complement(1566..2054)
                     /gene="coaD"
                     /locus_tag="PLK64_16535"
     CDS             complement(1566..2054)
                     /gene="coaD"
                     /locus_tag="PLK64_16535"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPV81401.1"
                     /translation="MATICVCPGSYDPVTNGHLDVIERASTLFDEVVVAVLYNPAKTG
                     TFPVARRIELIQQALGSRPRVRVESFAGRLLVDVCQDLGAGAIVKGLRGSTDFAFELP
                     MALMNRHLTGVETVFLPGNPAYEHVSSSLIKEVHALGGDITGLVPDVVLAALHEALPQ
                     RD"
     gene            complement(2076..>2414)
                     /locus_tag="PLK64_16540"
     CDS             complement(2076..>2414)
                     /locus_tag="PLK64_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HPV81402.1"
                     /translation="RLNAQALGFGDVVTVRAEPVERALLTGPDLTDGRADLAFLDPPY
                     DLAEDRLGAVLALLVLHRWLAPEALVVVERSARSPQPRWPSGLSAAGERRYGETKVWF
                     ADASGPDELA"
BASE COUNT          403 a          819 c          821 g          371 t
ORIGIN      
        1 ccaggcctgc tctgcggcgg cctgttcggc ttccttcttg ctgcgccccg ccccttcgcc
       61 aaggacctcg tgggccacaa ggactgccgc gacgaagctc ttctcgtgat ccggccctga
      121 ctcgacgagt cgatagtccg gcgcaccgag ggagccagcg gagcacagct cctgcaggct
      181 ggtcttccag tccagacccg cgcccaaccc ggccgcagtg gccagcagcg ggtcgaagag
      241 gtggtgcacg aactcagcag ccgcccgaag gccgccggag agatagatcg tgccgatgat
      301 ggcttccatc gtgtcggcaa ggatcgacgc cttgtcgcgt cctccggtcg tttcctcgcc
      361 gcgccccaaa cgcacgtagc caccgacgtc gatggtgcgg ccgacatcgg ccaacgcccg
      421 catgttgacg acggacgccc gcaacttggc cagttgcccc tcgggcagat ccgggtgcgc
      481 ggcatacaag gtgtcggtga tgaccaaccc gagaacagcg tcgccgagga actccaggcg
      541 ttcgttgttg ggcagcccac cgttctcgta cgcgtaagaa cggtgtgtga gggctcgttc
      601 aagcacctcg tcgtcgactg gtgtcccgat gaccccattg aggtagtcgc gcagggtcgt
      661 cagggacggt gccggtcctc ccccggtctg tcccatgacg gtgggctatc agccctggtg
      721 ttcggagcgc tcggccgccg ctacgtgccg acccttgtag gtgccgcacg acgggcacgc
      781 ctggtgcggc ggggtgggct gcgagcagcg cgggcaggtc gtcaggacgg ccgcagtggc
      841 cttccagttg gcccgccgcg agcgggtgtt ggagcgcgag gtcttccgct tggggacggc
      901 cacgtcaggt cctgttctct tcggggtcgt cgctcagcat cgtgccaagc gccgcccatc
      961 tcgggtcgag cacgtcgtga tggtggtccg ggtccagcgc tagggcgact ccgcactcgg
     1021 agcacaaccc tgggcagtcg acacggcaca ccggttggaa cggcagtgca ggcaccaccg
     1081 catcccggag cacgggctgc agatcgacca ggtcgtccac caactcgtac acctcggctt
     1141 cgtccgagtc ggcgtccacc tcatggtggt gcgccgcccg atcggcatac acgaacagct
     1201 cctggaagct cacctcgact gggaggctca cgggatccag gcaccgcacg catgcgccgg
     1261 ttgccgtggc ttggaccgaa ccgctcacga ggaccccttc ggagaccgac tccaaccgca
     1321 actcgatgtc gaccggtgaa ccggccggca cggcgacgat gtcggtgccc atctcctcag
     1381 ggacctccac ctgacgggtg tactcctgca tcgaccccgg ctggcgacga agctccctcg
     1441 tgtcgaacac gaggggcgcg cgagaatcga ggcgagacat gacttccgac tggttgaggg
     1501 ttcgatccac gccccggacg gacgtagacc gaagaactag gttaccggaa cggcgtcgaa
     1561 cgacgctaat cgcgttgcgg caacgcctcg tgcagggcgg ccagcacgac gtcggggacc
     1621 agcccggtga tgtcgccgcc gagcgcgtgc acctccttga tgagggagga cgacacatgt
     1681 tcgtatgccg ggttgcccgg gaggaagacc gtctcgaccc cggtgaggtg gcggttcatc
     1741 agcgccatcg gcaactcgaa tgcgaagtcg gtgctgccgc gcagcccctt gacgatcgcc
     1801 ccggcgccga ggtcctggca gacatcgacg agcagccgac ccgcgaacga ctcgacccgc
     1861 acccgaggtc gtgagccgag ggcctgctgg atcaactcga tccgacgcgc gaccgggaac
     1921 gtgccggtct tggcggggtt gtagaggacc gcgacgacga cctcgtcgaa gagggtcgag
     1981 gcacgctcga tgacgtcgag gtgaccgttg gtcacggggt cataagaccc ggggcacaca
     2041 cagatcgtcg ccacgttcgg gaggctagca gccgctcagg ccaactcgtc gggtccggag
     2101 gcatcggcga accagacctt cgtctcgccg taacgacgct cacccgcggc gctgagcccg
     2161 ctcggccagc gcggctgcgg cgagcgggct gatcgttcga cgacgacgag cgcctcgggc
     2221 gccagccacc ggtggagtac cagcagggcg agaacggcgc ccagtcggtc ctcagccagg
     2281 tcgtagggcg ggtcgaggaa ggccagatcg gcgcgaccgt cggtcaggtc agggccggtg
     2341 agcagggccc tctcgaccgg ctcggcccgt accgtcacca cgtcgccgaa gcccagcgcc
     2401 tgggcattga gccg
//
LOCUS       DAOMZY010000430         2407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_45109_length_2407_cov_5.346939, whole genome shotgun sequence.
ACCESSION   DAOMZY010000430 DAOMZY010000000
VERSION     DAOMZY010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45109_length_2407_cov_5.346939"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PLK64_16545"
     CDS             complement(<1..392)
                     /locus_tag="PLK64_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPV81403.1"
                     /translation="MRDSHYDGFAEAYAKQNETGLFNAYYEKPAMLALAGDVSGRRIL
                     DAGCGAGPTMAALRKAGASVVGFDVSAAMVDLARQRLGADADLRVADLAEPLPFGDAE
                     FDDVVASLVLHYLEDWSGGMLGCPDYVLA"
     gene            complement(809..1318)
                     /locus_tag="PLK64_16550"
     CDS             complement(809..1318)
                     /locus_tag="PLK64_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HPV81404.1"
                     /translation="MNSLTPGLDGGRRVLAPTGAALVRAVVEAVRGAAYNPSPTRAQA
                     EALLPFVEQARAVFEAADRRDTARAAGLVNALLVDSEARAALDEFDGEYHLHFHGPDT
                     TFARGWAAGIASALAMALGGNIYRRLGVCEAPACDRVYVDASKNSHKRFCSTRCQSRV
                     KAAAHRARR"
     gene            1395..>2407
                     /locus_tag="PLK64_16555"
     CDS             1395..>2407
                     /locus_tag="PLK64_16555"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV81405.1"
                     /translation="MSLSNEVTSVTRAVVLVRVVNRAGGFGMSFLGLRMARDLGMSLT
                     SVGLVLALFGLCTMPSRILGGVLTTRLGARSTIVVGLMGCAAAQLIIAFGSNIPAVIA
                     GVLTLGLAYEIIEPATQALVVEIVPGAKQASTFALLWSSIAVAGVVSGVLVALLAPWG
                     VSALFVADALSSLLAAAVAARLLPSTTSAVASPPWRSALTRRLIVWTGIGTLYAVLVM
                     LIVFMLPLAVEVAGWSPVVTGWLLAVAAAAAIGAQRLVARWEERLRPATMLVIGHVVL
                     AAGLALWATGELGFLVLGAVLEGASGAFCLGTYQAVAGRMAQPGLAAAVMTVFGLSWG
                     VAAV"
BASE COUNT          331 a          874 c          792 g          410 t
ORIGIN      
        1 gccaggacgt agtccgggca gccgagcatg ccgccggacc agtcctccag gtagtgcagc
       61 accaacgagg cgaccacgtc gtcgaactcg gcatccccga agggcaacgg ctcggcaaga
      121 tcggccaccc gcagatcagc gtccgcgccc agccgttgcc gggccaggtc gaccatggcc
      181 gcgctcacgt cgaacccgac gaccgaagcg ccggccttgc gaagagcagc catcgtcggg
      241 cccgcgccac acccggcatc caggatccgc cgacccgaga cgtcaccagc gagggcgagc
      301 atggcgggct tctcgtagta ggcgttgaag agccccgtct cgttctgttt cgcgtaagcc
      361 tccgcgaagc cgtcgtagtg gctgtccctc acccgtcatt cctctcgcgt tgcctcatga
      421 gttgtgcacc tccgcgtccc ctcgtccacc gtcagcgcct cgggcgggag gacccgacgc
      481 acagttcgca cgaacgttgg aacctgtcgc catacccgga ccggggttcc aacggtgatc
      541 cgcggcgggt tcaccagcgc caggttgtgc atggcgagca ccgagtctcc catcacgaat
      601 gcgccttctc ccacgaggcg cacagcaagg gcgtactggt cgagatcgct cgtcggcacg
      661 gtcgtatccc gatagaggcc gtgaccgacc tgttgaagga ctccccgccc tgcaagcttg
      721 cgcagggcca ccaacgggat tccttcatcg ttggcatcac gagtggagac caggccgtac
      781 tcccccggag gtgaggaagg cggcatactc accggcgggc tcggtgggcg gcggccttga
      841 cccggctctg gcagcgggtc gagcagaacc gcttgtgcga gttcttcgag gcatccacat
      901 agacccgatc acacgccggc gcctcgcaca cgccgagccg acggtagatg ttgccgccga
      961 gggccatcgc cagcgcagag gcgatccccg ccgcccagcc gcgggcaaac gtcgtgtcgg
     1021 gcccgtggaa gtgcaggtgg tattcgccgt cgaactcgtc cagcgcggcg cgcgcctcgg
     1081 agtcgaccag cagggcgttg accagtcccg ccgcccgcgc cgtgtcgcgg cggtcggccg
     1141 cctcgaacac cgctcgcgct tgctcgacga acggcagcag cgcctctgcc tgggcgcgcg
     1201 tcggcgacgg gttgtatgcc gccccccgca ccgcctcgac gaccgcacgg accagcgccg
     1261 ctccggtcgg cgccaacacg cgccgtccgc cgtcgaggcc gggggtcagg ctgttcacca
     1321 accccactgt caccgccaac aaccgatcta cgtgactgtt gaactgcatc tgaccagtca
     1381 cccaccctct ccgtgtgtca ctgtcgaatg aggttacatc agtcactcgg gcggtcgtgc
     1441 tcgtccgcgt cgtgaaccgg gccggcggct tcggcatgtc gttcctcggt ctgcggatgg
     1501 cgcgcgacct cggcatgtcg ttgaccagcg tcggcctggt cctcgcgctg ttcggactgt
     1561 gcaccatgcc ttcgcgcatc ctcggcggcg tgctcaccac ccgactcggc gcacgctcga
     1621 cgatagtcgt cggcctaatg ggttgcgccg cagcgcaatt gatcatcgcc ttcggctcga
     1681 acatcccggc ggtgatcgca ggcgtgctca ccctggggct ggcctacgag atcatcgaac
     1741 ccgctaccca ggcccttgtg gtcgagatcg tgcccggcgc gaagcaggcc tccaccttcg
     1801 cgctgttgtg gtcgagcatc gcggtcgccg gggtcgtctc gggcgtcctg gtcgccctgc
     1861 tcgcgccctg gggagtctcg gccctcttcg tcgccgacgc tctgtcaagc ctgctcgccg
     1921 cagccgtcgc cgcccgcctg ctcccctcaa cgaccagcgc cgtcgcctcg cccccgtggc
     1981 gctcggcgct cacccgtcgc ctgatcgttt ggacgggcat cggcaccctg tatgccgtgc
     2041 tcgtcatgct catcgtgttc atgcttccgc tcgcagttga ggtcgccggc tggagccctg
     2101 tcgtgaccgg ctggctcctc gcggtggctg cggcggcggc catcggggcc caacggctgg
     2161 tggcccggtg ggaggagcgg ctgcgcccgg ccacgatgct cgtcatcggc cacgtcgttc
     2221 tcgccgctgg attggccttg tgggcgaccg gcgagctcgg gttcctcgtg ctgggcgcgg
     2281 tcctggaggg cgcgagcggc gccttctgcc tcgggaccta ccaagcggtg gctggccgga
     2341 tggcccagcc agggctcgcg gctgcggtga tgaccgtgtt cgggctgtcg tggggtgttg
     2401 ccgcggt
//
LOCUS       DAOMZY010000431         2371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_46168_length_2371_cov_6.859672, whole genome shotgun sequence.
ACCESSION   DAOMZY010000431 DAOMZY010000000
VERSION     DAOMZY010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46168_length_2371_cov_6.859672"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PLK64_16560"
     CDS             <1..663
                     /locus_tag="PLK64_16560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPV81406.1"
                     /translation="DNLRYFAELYAVPARDQRARIGELLELVGLTGREKERVEGFSRG
                     MRQRLHIARGLLHDPALLFLDEPSIGIDPVGARELRQTIAGLRAAGKTILLTTHYMFE
                     ADELCDRIAVLSQGELVAEGTPAQLKARVTAGSVVEVETFSGVGPADVRGKVDAVGAL
                     PGVRSVVVDDRPDGETVVVQVEPGVDVTAAVLGTLTGIRVGRVVTREPTLEDAYVQLV
                     SS"
     gene            660..1466
                     /locus_tag="PLK64_16565"
     CDS             660..1466
                     /locus_tag="PLK64_16565"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV81407.1"
                     /translation="MSGVASGFMRGFMRGARVLGVGWILQLKILAVSAFDGILNIIYP
                     LFFATTALLMFRQSGDPQALVYAGLGAAVMGTWSAMATSASGALQGQRWQGTLELLVT
                     APTRVALAVLPITAALATVALYSLVATLIWGRVLFGIEVHIASPLAFAVALLVTLASL
                     ATFGFLLSVTVVRYRTGWALGNLLEYPGWLLCGFLVPLDLFPEWVRVIARALPPTWGM
                     DAIRAAAAGLDPWFDIAVCAGLGVGYAALGAFLSERLIDSARRHATLALT"
     gene            1463..>2371
                     /locus_tag="PLK64_16570"
     CDS             1463..>2371
                     /locus_tag="PLK64_16570"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPV81408.1"
                     /translation="MSAPQAAGPEVAGRGLAGPGAAGTQAARTSRHGRPEAVSRWDGI
                     RAAWRVLTVGGVISFRGLFNWLNPYVYVPTMLVAPIFQILLFVYIGRSAGVGTDAFYL
                     VGNAVQYASIPCLFAMANTIAGERWTQTLGIVLSTPANRVALFVGRSLPVIANGWFVA
                     MFSLFVGGWVLGVDLPVSAWPGIALTIAIASASCTGLGLLQAALALRVRSTAVLSNIV
                     FGVLLVFTGTNVALDSLPEWMATAGRLMPLTHAIEACREVVAGASLSAVAPLLLREGL
                     MGAVFAVLGLAALRWVEAESRRHATLE"
BASE COUNT          271 a          788 c          868 g          444 t
ORIGIN      
        1 gacaacctgc gctacttcgc cgagttgtat gccgtgcccg ctcgcgacca gcgcgcccgg
       61 atcggtgagc tgcttgagct tgtcggcctg accggccggg agaaggaacg cgtcgagggc
      121 ttctcccgag ggatgcgcca acgcctgcac atcgcccgcg gactgctgca tgacccggcg
      181 ctgctgttcc tcgatgagcc gtcgatcggc atcgacccgg tcggcgcccg cgagttgcgc
      241 cagaccattg cgggtctgcg cgctgcgggc aagacgatcc tgctgacgac ccactacatg
      301 ttcgaggccg acgaactctg cgaccggatc gccgtgctga gccagggtga gctcgttgcg
      361 gaagggacac cggcccagct caaggctcgg gtcaccgccg ggtcggtcgt cgaggtcgag
      421 acgttcagcg gggtcggccc ggccgacgtc cgtgggaagg tcgatgccgt cggtgccctg
      481 cccggggtgc ggtcggtcgt cgtcgatgac cggccggacg gcgagaccgt cgtcgtgcag
      541 gtcgagccgg gggttgatgt cacggccgcc gtcctcggga ccctcaccgg catccgggtg
      601 gggcgtgttg tcacccggga gccgacgttg gaggacgcct acgtccaact ggtgtcctcg
      661 tgagtggtgt cgcgagcggc ttcatgcgcg gcttcatgcg cggcgctcgg gtgctgggcg
      721 tggggtggat cctgcaactg aagatcctcg ctgtgtcagc cttcgacggc atcctcaaca
      781 tcatctatcc gctgttcttc gcgacgaccg cgctgctgat gttccgccag tccggcgacc
      841 cgcaggcgct cgtctatgcc gggctcggcg cggccgtcat gggcacctgg tcggcgatgg
      901 cgacctcggc cagcggcgca ctgcagggac aacgttggca gggcacgctc gaactcctcg
      961 tcacggcgcc cacccgggtg gctctcgccg tgctgccgat cacggccgcc ctggccacgg
     1021 tcgccctcta ctcgctggtc gccaccctga tttggggtcg ggtgctgttc ggcatcgagg
     1081 tccacattgc cagcccgctg gccttcgcgg tggccctgct ggtcaccctc gcgtccctgg
     1141 cgaccttcgg gttcctcctg tcggtcaccg tcgtgcgcta ccggaccggc tgggcgctgg
     1201 gcaacctgct ggaatacccc ggctggctgc tgtgcgggtt cctcgtgccc ctggacctgt
     1261 tccccgagtg ggtgcgcgtc atcgcccggg cgttgccgcc gacgtggggg atggacgcga
     1321 tccgggctgc cgcggccggg ctcgatccgt ggttcgacat cgccgtctgt gccgggttgg
     1381 gcgttgggta tgccgccctc ggcgcgttcc tgtccgagcg gctcatcgac tcggcgcggc
     1441 gacacgccac cttggcgttg acgtgagcgc gccacaggcg gcgggtcctg aggtggcggg
     1501 ccgagggttg gcgggcccag gggcggcagg gacccaggct gcgaggacga gtcggcacgg
     1561 gcgccccgag gcggtctcgc ggtgggacgg catacgtgct gcttggcggg tgctcaccgt
     1621 cggcggggtg atctccttcc gcgggttgtt caactggctc aacccctatg tctacgtgcc
     1681 gacgatgctc gtcgcgccga tcttccagat cctgctgttc gtctacatcg ggcgcagcgc
     1741 gggcgtcggg acggatgcgt tctacttggt cggcaacgcg gtgcaatacg cgtcgatccc
     1801 gtgtctgttc gcgatggcca acacgatcgc gggggagcgc tggacccaga ctctcgggat
     1861 cgtcctgtcg acgccggcca accgggtcgc gctcttcgtc gggcgctcgc tgccggtcat
     1921 cgccaacggc tggttcgtcg cgatgttcag tctgttcgtc ggaggctggg tgcttggcgt
     1981 cgatctgccg gtcagcgcgt ggccggggat cgcgctgacc atcgcgatcg cctcggcctc
     2041 gtgcacgggg ttggggttgc tccaggccgc cctggccttg cgggtgcgta gcaccgcggt
     2101 cctgtccaac atcgtctttg gggtgctgct ggtcttcacc gggaccaacg tggcgctgga
     2161 cagccttccc gagtggatgg cgacggccgg gcgacttatg ccgctcaccc atgccatcga
     2221 ggcctgccgc gaggtggtgg ccggagcgag cctgtcggcg gtcgccccgt tgctgctgcg
     2281 cgaggggttg atgggtgccg tgttcgcggt gctcgggctg gcggcgttgc ggtgggtgga
     2341 ggccgagagc cgccggcacg ccaccctcga a
//
LOCUS       DAOMZY010000432         2366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_46330_length_2366_cov_5.337084, whole genome shotgun sequence.
ACCESSION   DAOMZY010000432 DAOMZY010000000
VERSION     DAOMZY010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46330_length_2366_cov_5.337084"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="PLK64_16575"
     CDS             <1..757
                     /locus_tag="PLK64_16575"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HPV81409.1"
                     /translation="ATGEVDRSASVAENHLVLRSIGKDPAETAVIAKWNTLAAPMAAR
                     IVGSHVEDILGDSNGNRGIETPMADLVADAWLYGTASAANGGAQIAFQNVGGTRASFR
                     VNSTPNGEQPGQITYAEAYAVVPFSNVMVTIDLTGEQLRQALEQQFVPTRSRQWLALG
                     VSAGFSYAWDALAPQGSKVVAGSMTLNGVPMSPTATYRVALPNFLADGGDDFLAFKAG
                     TNRLGGGEDLAMLVSYLQSHPGLTAPGSRVDGL"
     gene            complement(840..2345)
                     /locus_tag="PLK64_16580"
     CDS             complement(840..2345)
                     /locus_tag="PLK64_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HPV81410.1"
                     /translation="MTSEQQVKTDNRPRPTTEQFRAFVREGWAPRSTELPARADVAPY
                     AAARREALSAAYPGERLVIPAGGLKVRSNDTDYVFRPHTAFAHLTGFGGDREPDAVLV
                     LHPRSEADGGGHDAVLYFRPMAGRDTEEFFADARYGEFWVGARPTLADIEAEFGLVAR
                     HIDDLPDALAKDAGAVTLRVVRDADSDVAALVDQARTEAGVEVTEEITEADVALAAYL
                     SQLRMTKDEWEIQQMRDAVAATKVGFEAVIADLRSAVERGRGERWVEGLFGLHARHQG
                     NGVGYDSICAAGDHANTLHWIKNTGEINEGELLLLDAGVEVDSLYTADITRTLPVDGT
                     FTDAQREIYDAVYAAQEAAMAAIRPGAKFSDIHAAAIRVIAQHVHAWGLLPEGIDVEA
                     SLDTETGQYHRRWMVHGTSHHLGIDVHDCALATREEYMDAELVPGMVLTVEPGLYFKA
                     DDLLAPERFRGQAVRIEDDVVVTETGYENLSAGMPRRAEDVEAWIAQVQGR"
BASE COUNT          345 a          815 c          838 g          368 t
ORIGIN      
        1 ggccaccggt gaggtcgacc ggtccgcgtc ggtggcggag aaccacctcg tgctccgctc
       61 gatcgggaag gacccggcgg agaccgcggt catcgccaag tggaacaccc tggccgcgcc
      121 catggcggcc cggatcgtcg gctcgcacgt cgaggacatc ctgggcgact ccaacggcaa
      181 ccgaggcatc gaaacgccga tggccgatct ggtcgccgac gcctggctct acggcactgc
      241 ctcggcggcc aacggcggcg cgcagatcgc cttccagaac gtcgggggca ccagggcgag
      301 cttccgggtc aactcgaccc cgaacggcga gcaaccgggg cagatcacct atgccgaggc
      361 gtacgccgtt gtgcccttca gcaacgtgat ggtgacgatc gacctcacgg gtgagcagct
      421 gcgtcaggcg ctggagcaac agtttgtccc cacgcgtagt cgtcagtggc tcgctctcgg
      481 agtgtcggcg ggcttcagtt acgcctggga cgccctggca ccgcaaggct cgaaggtcgt
      541 ggccggatcg atgaccctca acggggtgcc catgtcgccg acggccacct accgggtcgc
      601 gttgccgaac ttcctggccg acggaggcga cgacttcctg gccttcaagg cgggcaccaa
      661 ccggctcggt ggcggcgagg acctcgccat gctggtgagc tacctgcaga gccacccggg
      721 cctcaccgca ccggggagcc gggtcgacgg cctctgacgg gaccgcccgg ccaactgact
      781 gagggccgca ccccgatggg ggtgcggccc tcggtcgcat gcgcaggctt ccgaagcggt
      841 cagcgaccct ggacctgagc gatccacgct tcgacgtcct cggcccgccg cggcatacca
      901 gcagagaggt tctcgtagcc ggtctcggtg acgacgacgt cgtcctcgat ccgcacggcc
      961 tggccgcgga aacgctccgg ggcgaggaga tcatcggcct tgaagtagag ccccggctcg
     1021 acggtgagga ccattccggg gaccaactcg gcgtccatgt attcctcccg agtggccagc
     1081 gcacagtcgt gcacgtcgat gcccaggtgg tggctggtgc cgtggaccat ccagcgtcgg
     1141 tggtactgac cggtttcggt gtccagggac gcctccacgt cgatcccctc ggggagcagc
     1201 ccccacgcgt ggacatgctg ggcgatgacc cggatggccg ccgcgtggat gtcggagaac
     1261 ttcgctcccg ggcggatcgc cgccatggcc gcttcctgcg cggcatacac ggcgtcatag
     1321 atctcgcgtt gcgcgtcggt gaaggtgccg tcgacgggca gggtgcgagt gatgtcggcg
     1381 gtgtagaggc tgtcgacctc gaccccggca tcgagcagga ggagctctcc ctcgttgatc
     1441 tcgccggtgt tcttgatcca gtgcagggtg ttggcgtggt cgccggccgc gcagatcgag
     1501 tcatagccga ccccgttgcc ctggtggcgg gcgtgcagcc cgaagaggcc ctcgacccag
     1561 cgctcgccgc gcccgcgctc gaccgccgag cgcaggtcgg caatgaccgc ctcgaagccc
     1621 accttcgtcg ccgcgaccgc gtcgcgcatc tgctggatct cccactcgtc cttggtcatt
     1681 cgcaattgcg agaggtatgc cgcgagcgcc acgtccgcct cggtgatctc ctcggtcacc
     1741 tcgacaccgg cctcggtgcg agcctggtcg acgagggccg cgacgtcact gtcggcgtca
     1801 cggacgaccc gcagggtgac ggcgccggcg tccttggcca acgcgtcggg caggtcgtcg
     1861 atgtggcgtg ccacgagccc gaactcggct tcgatgtcgg ccagggtcgg ccgcgcgcca
     1921 acccagaact cgccgtagcg ggcgtcggcg aagaactcct cggtgtcgcg gccggccatc
     1981 ggacggaagt agaggacggc gtcgtgcccg ccgccgtcgg cctcactgcg ggggtgcagc
     2041 accaggacgg catctggctc gcggtcgccc ccgaagccgg tgaggtgggc gaacgcggtg
     2101 tgcggccgga agacgtagtc ggtgtcgttg gaccgcacct tcaggccgcc ggcggggatg
     2161 acgagccgtt ccccggggta tgccgcgctg agagcctcgc ggcgcgccgc ggcatacggg
     2221 gccacgtcgg cccgcgcggg gagctcggtg gagcgtggtg cccacccctc gcgcacgaag
     2281 gcgcggaact gctcggtggt cggccgcggg cggttgtcgg tcttgacctg ctgttcactc
     2341 gtcacccgac caatgctcgc atgtcg
//
LOCUS       DAOMZY010000433         2354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_46675_length_2354_cov_5.859504, whole genome shotgun sequence.
ACCESSION   DAOMZY010000433 DAOMZY010000000
VERSION     DAOMZY010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46675_length_2354_cov_5.859504"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PLK64_16585"
     CDS             <1..678
                     /locus_tag="PLK64_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HPV81411.1"
                     /translation="DYSQIEMRVMAHLSGDDGLIEAFRSGEDLHSFVASRVFGVDPTA
                     VTPEMRSKIKAMSYGLAYGLSAFGLSRQLGISTGEAKTLMDEYFERFGEVRDYLHRLV
                     ADARGTGFTATMLGRRRYLPDLLSDNRQRREMAERMALNAPIQGSAADIMKVAMLRVD
                     EAVREQRLASRMLLQVHDELVIEVAPGERDQVEELVRREMGAAVALTVPLDVSVGVGR
                     TWHDAAH"
     gene            728..1420
                     /locus_tag="PLK64_16590"
     CDS             728..1420
                     /locus_tag="PLK64_16590"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPV81412.1"
                     /translation="MDLLLIRHAQSENNLVWAHTGASVGRVPDPLLTPLGERQAAAVA
                     QALAQGRYAAPPSVLYASLMSRAVLTAAPIAAALDLAMHGHPDLFEVGGPLDWSGHED
                     VPRRAHRGAGRASLRELSPRLVLPDDAGEHGWWDGPVEVETDVPARAHRVIDSVLARH
                     GGTDAVVGLVTHGHFSQFLVRALLGIPTMTGWVDILNTSVSRFSDVDVPGRTAAAWIN
                     RVEHLLPDEVSD"
     gene            complement(1377..>2354)
                     /locus_tag="PLK64_16595"
     CDS             complement(1377..>2354)
                     /locus_tag="PLK64_16595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81413.1"
                     /translation="RLAAVLSAAALVLVPGAAYLLRVDVGAALAVATAAAPITLTFAV
                     QGLLQGRERFGALATVAALVGITRVSAAVLAAAGDLGPDAVMWLFTAGWALTLYIAGF
                     LLHRDGGPHLFKAARHAWGSAPSRAIFRQSVTAVLPMSGLLVLSSLDMLLARHFLPED
                     LSGGYAIGAVFEKVAFWGPTFLATLYYPRMARAADRARAIRDALLLTASVGAVGVLAA
                     FLLGGPLVAVVGGAEYAALAPLAWLFCTLGVTLALVQVLVYADLAAGRHRAGAAVWLA
                     LAASTAVVAGRHASIEQVVTGVLGCLLVVALVSAWLSRIPRRGAGARRG"
BASE COUNT          382 a          816 c          857 g          299 t
ORIGIN      
        1 gactacagcc agatcgagat gcgggtcatg gcccacctgt ccggcgacga cgggctcatc
       61 gaggcgttcc gctcgggtga ggacctgcac tcgttcgtgg cgtcccgggt cttcggggtc
      121 gacccgacgg cggtgacacc ggagatgcgc tccaagatca aggcgatgtc ttacggcttg
      181 gcctacggcc tgtcggcgtt cgggttgtcc cgccagttgg ggatctccac cggcgaggcc
      241 aagacgctca tggacgaata cttcgaacgc ttcggtgagg tccgcgacta cctgcaccgc
      301 ctcgtcgccg atgctcgggg tacggggttc accgcgacga tgctcgggcg ccggcgctac
      361 ttgcccgacc tgctctcgga caaccgacaa cggcgcgaga tggccgagcg gatggccctc
      421 aacgccccga tccaggggtc cgccgccgac atcatgaagg tcgccatgtt gcgggtcgac
      481 gaggcagtgc gggagcagcg gctcgcctcg cggatgctgc tgcaggtcca cgacgagttg
      541 gtcatcgagg tggcgcctgg cgagcgggac caggtcgagg agttggtccg ccgcgagatg
      601 ggcgcagcgg tcgccctcac ggtgccgctg gacgtgtcgg tcggcgtcgg gcgcacctgg
      661 cacgacgccg cgcactgagg ccgagaacaa cgaacgccgc gaggcgaagg gcgagggaac
      721 ggcatacatg gatctgctgc tcatcaggca cgcgcagtcg gagaacaacc tcgtgtgggc
      781 gcacacgggc gcgtcggtcg ggcgagtgcc cgacccgctg ctcacgccgt tgggggagcg
      841 gcaggccgcg gcggtcgctc aggcgctcgc gcaaggccgg tatgccgccc cgccgtccgt
      901 gctgtatgcc agcctcatga gccgcgccgt tctcacggcg gcaccgatcg ccgcggcgct
      961 cgacctcgcc atgcacgggc atccggacct cttcgaggtc ggcgggccac tggactggag
     1021 cggccacgag gacgtgccgc gtcgagcgca tcgtggggcg gggcgggcgt cgctgcgcga
     1081 gctgtcaccc cggctggtcc tgcccgacga cgcgggtgag cacggctggt gggatgggcc
     1141 ggtggaggtc gagaccgacg ttcccgcccg ggcccaccgg gtgatcgact cggtgctggc
     1201 ccgacacggt ggcaccgatg cggtggtcgg gttggtgacg cacgggcact tcagccagtt
     1261 cctcgtccgg gcgctgctgg gcatccccac gatgacgggg tgggtcgaca tcctcaacac
     1321 ctcggtgtcg cgcttctccg atgtcgacgt gccggggcgc acggccgcgg cgtggatcaa
     1381 ccgcgtcgag cacctgctcc ccgacgaggt atccgactga gccaggcgct gaccaacgca
     1441 accaccagga ggcagccgag caccccggtg acgacctgct cgatcgaggc gtgccggccg
     1501 gcgacgacgg cggtcgatgc cgccaaggcg agccagaccg ccgcccccgc gcgatgccgc
     1561 ccggcggcaa ggtcggcgta gacgagcacc tgaaccaacg cgagcgtcac cccgagagtg
     1621 cagaacaacc aggccagggg tgccagcgcg gcatactcgg caccgccgac gaccgcgacc
     1681 aggggccccc cgagcaggaa ggcggccagc acaccgaccg cgccgacgga tgccgtgagg
     1741 agcagggcat ctctgatggc acgagcccgg tcggctgccc gggccatccg cgggtagtag
     1801 agggtcgcca ggaaagtcgg gccccagaac gcgacctttt cgaacaccgc gccgatcgcg
     1861 tagcccccgc tgaggtcctc gggcaggaaa tgccgagcca ggagcatgtc gagactggac
     1921 aggacgagca gacccgacat cggcagcacc gccgtcactg actggcggaa gatcgcccgc
     1981 gagggcgccg agccccaggc gtggcgcgcg gctttgaaga ggtgtggccc accatcgcgg
     2041 tgcagcagga acccggcgat gtacaacgtc agcgcccacc cggcggtgaa cagccacatg
     2101 acggcgtcgg ggccgaggtc acctgccgcg gcaaggacgg cggcgctgac ccgggtgatc
     2161 ccgacgagtg cggccacggt cgccagcgcc ccgaagcgct cgcggccctg caacaacccc
     2221 tggacggcga aggtcaacgt gatcggtgcc gccgcggtgg ctacggccag cgccgccccg
     2281 acgtccaccc gcaagaggta tgccgccccc ggcaccagga ctagcgcggc cgccgacagg
     2341 accgcggcga gccg
//
LOCUS       DAOMZY010000434         2341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_47075_length_2341_cov_7.124672, whole genome shotgun sequence.
ACCESSION   DAOMZY010000434 DAOMZY010000000
VERSION     DAOMZY010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47075_length_2341_cov_7.124672"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..865)
                     /locus_tag="PLK64_16600"
     CDS             complement(11..865)
                     /locus_tag="PLK64_16600"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPV81414.1"
                     /translation="MSDLSYVQSVILGIVEGLTEFLPVSSTGHLTVTEKLLGLDVADP
                     AVTAYTAIIQLGAIVATFVYFWQDIVRLARAWFRGITRPDERALPDYALAWAVIVGSI
                     PVALVGFALRKVIAGPLRSLWVVAAALIIWSVVMWLAESRHAKLVREGAERGESTVTM
                     KDGLIIGLVQCFSLVPGVSRSGATISAGLFRGIDRVTATRLSFFLAIPALTAAGLFEA
                     ATTDLKGLGIGQMAVGVVVAFGVAYASIAWLLKFVAGHKLTAFVLYRVVLGALIVVAL
                     GAGWLTAQ"
     gene            complement(872..1939)
                     /locus_tag="PLK64_16605"
     CDS             complement(872..1939)
                     /locus_tag="PLK64_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HPV81415.1"
                     /translation="MRLGLNLGYWGAGNDQSNVTLAEEADRLGYSVAWVAEAYGSDSP
                     TVLSWVGARTQRIHLGAAVMQIPARTPAMTAMTAATLDSLSGGRFRLGLGVSGPQVSE
                     GWHGVRFDAPLGRTREYVDIVRMALRRDVVSYEGRHFTLPLPDGPGKALKLGVRPVRD
                     GQIPIYLAAVGPKNLELTGEIADGWLAIFFSPEFAGDLIGSLETGARRAGKGADGTAP
                     LAGFDIVPTVPVVVTDDLDYAYAAVAGYAALYIGGMGSREKNFYNQLACRMGFEKEAK
                     LIQDLYLDGKARDAAAAVPREFLDATSLLGPRERIRDRLAAYQEAGVTTLSVAPYGPS
                     MEHRRAIVHTMAEVAQEAGLG"
     gene            1991..>2341
                     /locus_tag="PLK64_16610"
     CDS             1991..>2341
                     /locus_tag="PLK64_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPV81416.1"
                     /translation="MLSRHLGRSGLAVSRLGLGTLTWGVEVDEEGARDQLTAFVAAGG
                     TLVDTAHGYGAGAAETMLGTLLKARAVPREDLVLVTKAGIRQEGDRRIVDVSRRGLLR
                     ELDGSLARLGTDHVD"
BASE COUNT          369 a          806 c          844 g          322 t
ORIGIN      
        1 cgggcttgcc tcactgggcg gtgagccagc ccgctcccag cgcgacgacg atgagcgcgc
       61 cgagcacgac gcggtagagg acgaacgccg tcaacttgtg cccggcgacg aacttgagca
      121 gccaggcgat cgaggcatag gcgacgccga aggccacgac cacgccgacg gccatctgcc
      181 cgatgcccag ccccttcagg tcagtggtgg ccgcctcgaa caggcccgca gcagtcagcg
      241 cggggatggc gaggaagaag gacagccggg tcgcggtcac tcggtcgatc ccgcggaaca
      301 gcccggccga gatcgtcgcg ccggagcggc tgacgccggg gacgagcgag aagcactgca
      361 cgagcccaat gatcagcccg tccttcatcg tcaccgtcga ctcgccacgc tcggcgccct
      421 cgcgcaccaa cttggcgtgc cgcgactcgg ccagccacat gacgacgctc cagatgatga
      481 gtgccgcggc gaccacccag aggctgcgca gcggcccggc gatcaccttg cgcagggcga
      541 agcccacgag cgccacaggg atcgagccga cgatgaccgc ccacgccagc gcgtagtccg
      601 gcagcgcccg ctcgtcgggc cgggtgatcc cgcggaacca cgcgcgcgcc agccgcacga
      661 tgtcctgcca gaagtagacg aaggtcgcca cgatcgcgcc gagctggatg atcgcggtgt
      721 atgccgtgac cgccgggtcc gcgacgtcca gcccgagcag cttctcggtc accgtgaggt
      781 gcccggtcga ggacacgggc aggaactcgg tgagcccctc gacgatgccg aggatgaccg
      841 actggacgta gctgagatcc gacacgaggg atcagcctag cccggcctcc tgcgccacct
      901 cggccatcgt gtggactatg gcgcgccggt gctccatcga cggcccgtag ggcgcgaccg
      961 acagggtggt caccccggcc tcctggtatg ccgccaaccg gtcccggatc cgctcgcgcg
     1021 gcccgagcag gctggtggca tcgaggaact cccgaggcac cgcggcggcg gcatccctcg
     1081 ccttcccgtc gaggtagagg tcctggatga gcttggcttc cttctcgaac cccatccggc
     1141 aggcgagttg gttgtagaag ttcttctccc gcgaccccat gcccccgatg tagagggcgg
     1201 cataaccggc gaccgcggca tacgcgtagt ccaggtcgtc cgtgacgacg acggggacgg
     1261 tgggcacgat gtcgaagccg gcgagcggcg cggtcccgtc ggcgcccttg cccgcgcggc
     1321 gggcgccggt ctcgagtgac ccgatgaggt caccggcgaa ctccggcgag aagaagatcg
     1381 ccagccagcc atcggcgatc tcgccggtga gctcgaggtt cttggggccg accgcggcca
     1441 ggtagatggg gatctgcccg tcgcgcaccg gtcgcacgcc gagtttgagg gccttgccgg
     1501 gcccgtccgg cagcggcagg gtgaagtggc ggccctcgta gctaacgacg tcgcgtcgca
     1561 gcgccatccg cacgatgtcg acgtattcgc gggtgcggcc cagcggggcg tcgaagcgca
     1621 caccgtgcca gccttcgctc acctgcggcc cgctgacgcc caggccgagc cggaaccggc
     1681 cgccggagag cgagtcgagc gtggccgcgg tcatggcggt catcgccgga gtgcgggccg
     1741 ggatctgcat gacggccgcg ccgaggtgga tgcgctgggt gcgggcgccg acccaggaca
     1801 gcacggtcgg agagtccgag ccgtaggcct cggcgaccca ggccaccgag tagcccaacc
     1861 gatcagcctc ctcggccagc gtgacgttgg attggtcgtt gccggcaccc cagtagccga
     1921 ggttcagtcc gaggcgcatc gtgtgggtca ccggaccagg ctatcggcgc gggcggatag
     1981 gttgtgggtc atgctttccc gtcacctggg ccgcagcggc ttggctgtct cgcgcctggg
     2041 cctgggcacc ctcacgtggg gtgtcgaggt cgacgaggag ggcgcccgcg atcagctcac
     2101 ggccttcgtc gcggccggcg gcacgctggt ggacacggct cacgggtatg gcgcaggggc
     2161 ggccgagacg atgctcggca ctctgctcaa agcccgcgcg gtgccgcgcg aggatctcgt
     2221 cctggtcacc aaggccggca tccggcagga gggcgaccga cggatcgtcg acgtgtcccg
     2281 acgtgggttg ttgcgtgagt tggacgggtc actggcgcgg ttgggcaccg atcacgtcga
     2341 c
//
LOCUS       DAOMZY010000435         2334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_47312_length_2334_cov_4.270294, whole genome shotgun sequence.
ACCESSION   DAOMZY010000435 DAOMZY010000000
VERSION     DAOMZY010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47312_length_2334_cov_4.270294"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1349)
                     /locus_tag="PLK64_16615"
     CDS             complement(54..1349)
                     /locus_tag="PLK64_16615"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPV81417.1"
                     /translation="MSTTLPARFSLDQPGRGGGAPMATWEQIVVDLPLVAATMRTYLD
                     QVAVVLRPGSVTNTDQCLRSFATYLLLHHHEISRVRDIGRAHVEGFKLWLAARPGRSG
                     TPRATNATLAHRLGTLRMFFLRIDEWGWDDAPTRVPMFPGDVPKQHHALPKALDDQTA
                     ARLLTAAQADKRQLVGLTVEFLLRTGLRVGEFTALAADAVVLIGDTHWLHVPVGKLHE
                     DRYLPLHPRLVELVTNYRAAHVPDGHRLLLPRENGKGIDRHMVTRFINKAGAAAGLGH
                     IHPHQLRHTLATQAINRGMSLEAIAAMLGHKSMDMTLVYAKIANRTVAEEYFTVAEKV
                     DALYARPAELPADDLGPNMARLNREHSRMLGNGYCIRPQIMDCIYETICESCTFFQTT
                     IEFRPTLLAQRDDACAKGQIRRAEIYDNLLTGLDTHEAS"
     gene            complement(1346..>2334)
                     /locus_tag="PLK64_16620"
     CDS             complement(1346..>2334)
                     /locus_tag="PLK64_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPV81418.1"
                     /translation="VLATWFDLKVFFAVVGKPVAAVRPVDVLGFISAQRAGLPSLPEP
                     HLLPVIASADYVGVSLRTIRRRVSTIAGLYGFLAARGDVSANPVPRGLPTRREKARPS
                     QGVPLVRAPRMLPRILSPVEVDALTGALRSHRDRAMVAAMLLGGLRRCEVLGLRLGDV
                     RIGERQLFIADGKGGHQRLVPVSPRFFDHLGVYLDKERPPEAETDHVFLVLKRPHRGR
                     PLSAAGLDQVLASARERAGLAHGSCHELRHTCLTRLREAGMALEAVQAQAGHASIEST
                     RIYLHLADDWLASQYRRAAEVIDAQVYAVGSPPQRTSVAEPQVSIPGFRRLR"
BASE COUNT          387 a          749 c          833 g          365 t
ORIGIN      
        1 gtcgggatat cgccacgcgc gtccgctcat cggcattatg tgggtatcgg ttgtcaagag
       61 gcttcgtgcg tgtcgaggcc ggtgagcagg ttgtcgtaga tctcggcgcg gcggatctgg
      121 cccttggcgc aggcgtcgtc gcgttgggcg agcagggtgg ggcggaactc gatggtggtc
      181 tggaagaacg tgcaggactc gcagatcgtc tcgtagatgc agtccatgat ctggggtctg
      241 atgcagtagc cgttgccgag catgcgggag tgttcgcggt tcaggcgggc catgttcggc
      301 ccgaggtcgt cggcggggag ctcggcgggg cgggcgtaga gggcgtcgac cttctccgcg
      361 acggtgaagt attcctcggc gacggtccgg ttggcgatct tggcgtagac cagggtcatg
      421 tccatcgatt tgtggccgag catggcggcg atcgcttcga gcgacatgcc gcggttgatg
      481 gcctgggtgg cgagggtgtg gcgtagctgg tgggggtgga tgtggccgag tccggcggcg
      541 gcgccggcct tgttgatgaa gcgggtgacc atgtgccggt cgatgccctt gccgttctct
      601 cggggcagca gtagtcggtg gccgtcgggg acgtgggccg cgcggtagtt ggtgacgagt
      661 tcgaccagcc tcggatgcag gggcaggtag cggtcctcgt ggagcttccc gaccgggacg
      721 tggagccagt gcgtgtctcc gatcaggacg acggcgtcgg cggccagcgc ggtgaactcg
      781 ccgacccgta ggccggtgcg caggaggaac tcgacggtca ggccgacgag ttgtcgcttg
      841 tcggcctggg cggcagtgag gagccgggcc gcggtctggt cgtcgagcgc tttggggagt
      901 gcgtggtgtt gcttcgggac gtcgccgggg aacatcggca cccgggtcgg ggcgtcgtcc
      961 caaccccatt catcgatgcg taggaagaac atccgcagcg ttcccagccg gtgagccagg
     1021 gtggcgttcg tggcgcgggg ggtgccggag cgtccaggtc gggcggccag ccagagcttg
     1081 aatccctcga catgcgcccg gccgatgtcg cggacgcggc tgatctcgtg gtggtggagc
     1141 agcaggtagg tcgcgaacga acgcaggcac tggtcggtgt tggtcacgct gccgggacgc
     1201 agcacgaccg ctacctgatc caggtaggtg cgcatcgtcg cggcgaccag cggcaggtca
     1261 accacaatct gctcccaggt cgccatcggc gccccgccgc ctcgcccggg ctggtccagg
     1321 gagaaccggg ccggcagcgt cgtgctcatc gcaacctcct gaaccccggg atgctcacct
     1381 gcggctccgc gacgctcgtg cgctgtggtg gtgagccgac ggcgtagacc tgggcatcga
     1441 tcacctcagc ggcgcgccga tactgcgatg ccagccaatc atcggccaga tgcagataga
     1501 tcctggtgga ctcgatcgag gcatgcccgg cctgggcctg gaccgcctcc aacgccatcc
     1561 cggcctcccg cagccgggtc aaacaggtat gccgcagctc gtgacacgat ccgtgcgcca
     1621 atccggcccg ctcccgcgcc gaggccagca cctgatccag acccgccgcc gacaacggcc
     1681 gcccgcgatg aggacgcttc agcaccaaga acacgtgatc ggtctcggcc tccggaggtc
     1741 gttccttgtc cagatagacg cccaggtggt cgaagaaccg cggcgagacc gggaccagac
     1801 gttggtggcc gcccttgccg tcggcgatga acaactgccg ctcgccgatg cggacatccc
     1861 ccaaccgcag ccccaggacc tcgcagcgcc gcagcccgcc caacaacatc gccgccacca
     1921 tcgcccgatc acggtggctg cgcaacgcac cagtcaacgc atcgacctcg acaggtgaga
     1981 ggatccgcgg caacatccga ggagcacgaa ccagcggaac accttgcgag ggccgggcct
     2041 tctcacgacg ggtcggcaac ccgcgaggga ccggattggc gctcacgtca ccacgcgcag
     2101 ccaggaagcc atacaagccg gcgatcgtcg agacccgacg ccggatcgtc cgtaacgaca
     2161 cccccacgta atctgccgac gcgatcaccg gcagaaggtg tggctcgggc agggacggca
     2221 gacccgcgcg ctgggccgaa atgaaaccca acacgtcgac cggacgcacc gcagccaccg
     2281 gcttacccac cacggcgaag aagaccttca ggtcgaacca cgtcgccaac accg
//
LOCUS       DAOMZY010000436         2333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_47337_length_2333_cov_5.079895, whole genome shotgun sequence.
ACCESSION   DAOMZY010000436 DAOMZY010000000
VERSION     DAOMZY010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47337_length_2333_cov_5.079895"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..1929)
                     /locus_tag="PLK64_16625"
     CDS             complement(202..1929)
                     /locus_tag="PLK64_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HPV81419.1"
                     /translation="MTTAFDYDVVIVGSGFGGSVAALRLTEKGYSVAVLEAGPRFEDA
                     DLPDTSFDVRNYLFAPSLGCYGIQRIDLLNDCLILSGAGVGGGSLVYANTLYQPPAAF
                     YDDPQWRDITDWKTELAPYYDQATRMLGVITNPWHTPSDEVIRQVAEEMGKGDTFRYT
                     PVGVLFPRDGESKGQPVDDPYFGGEGPRRHTCTGCGECMTGCRHNAKNTLVKNYLYLA
                     EKHGAHVHPLTTVTRVVPRHGGGYDVHTTNTRAKTKRRAARQSRTYAAQQVIFAASAL
                     GTQRLLHTMKFTGELPRLSDTLGRLTRTNSESILGAGAPKDDPRDFTEGVAITSSFHP
                     DEHTHIEPVRYGRGSNVMSLLTSVLTDDLGDTPRWRVWLRQSWHERSTVLRLYDFKHW
                     SEHTVIALVMQSLDNSINTFPKRNRVTGKITLSSEQGHGAPNPTWIPLANEAVRRMAR
                     NVGGFPAGNIGEQFHRPLTAHFIGGCPIGKDAEHGVVDAYHRVFGYDGLHIVDGSAIS
                     ANLGVNPSLTITAQAERAMAYWPNKGQTDARPALGTAYQGIAPVEPAKPIVPASAPAA
                     LRLPIVGIT"
     gene            complement(1930..>2333)
                     /locus_tag="PLK64_16630"
     CDS             complement(1930..>2333)
                     /locus_tag="PLK64_16630"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPV81420.1"
                     /translation="DETFGPVISVYPFTTEDEAVDLANAGDYGLNAAVFSRDNKRARL
                     IANRIKCGTVNINEAFSATFASLDAPMGGMRSSGIGRRQGAEGIHRYTEVQSVATQYL
                     LRFGPHWGLTDEAYAAVMTGAVKLLTRLGRA"
BASE COUNT          352 a          728 c          853 g          400 t
ORIGIN      
        1 gccttcccgg ggtgcgccac gacatcgcgg tcatcgagag cggtgacgcg tgggtcgcgg
       61 tcgccgccct ggccaccgac ctcatggacg gccccgtcga tcggggcacg tcgatctacc
      121 ccacgtatgc cgcgatcggc gagacggtcg ctgccctcgt caccgcctga acggaggccg
      181 atcgggcgca aacgcggtcg ctcaggtgat gccgacgatc ggcagacgca gggctgccgg
      241 agccgacgcc gggacgatcg gcttagccgg ctcgaccggt gcgatccctt ggtatgccgt
      301 gcccagcgcc ggccgcgcgt cggtctgacc cttgttgggc cagtaggcca tggcccgctc
      361 ggcctgagcg gtgatggtca acgacggatt gaccccgagg ttggccgaga tcgcggaccc
      421 gtcgacgatg tgcagaccgt cgtaaccgaa gacccggtgg taggcatcga cgacgccgtg
      481 ctcggcgtcc ttcccgatcg ggcagccacc aatgaagtgc gccgtcagcg gccgatggaa
      541 ctgctcgccg atgttgccgg ctgggaagcc gccgacgttc cgagccatcc gtcggaccgc
      601 ctcgttcgcc aacgggatcc aggtcggatt gggagcgccg tgcccctgct cggagctcaa
      661 cgtgatcttg ccggtgaccc ggttgcgctt ggggaaggtg ttgatcgagt tgtccagcga
      721 ctgcatgacc aacgcgatga cggtgtgctc cgaccagtgt ttgaagtcgt agagccggag
      781 cacggtggat cgctcgtgcc acgactggcg cagccagacc cgccaccggg gcgtgtcgcc
      841 caggtcgtcg gtgagcaccg aggtgagcag cgacatgacg ttggagccgc ggccatagcg
      901 gaccggctcg atgtgggtgt gctcgtccgg atggaacgac gaggtgatcg ccacaccctc
      961 ggtgaagtcg cgcgggtcgt ccttgggtgc gccggcgccg aggatcgact cggagttggt
     1021 ccgggtcaac cggcccagag tgtcggaaag acgggggagt tcgccggtga acttcatcgt
     1081 gtgcaggagg cgttgagtcc cgagtgccga cgccgcgaag atcacctgct gcgcggcata
     1141 cgtgcggctc tgacgcgctg cgcggcgctt ggtcttcgcg cgggtgttag tcgtgtggac
     1201 gtcgtatccg ccgccgtggc gggggacgac gcgggtcacg gtcgtcagcg ggtggacgtg
     1261 cgcgccatgc ttctcggcga ggtagaggta gttcttcacg agggtgttct tcgcgttgtg
     1321 gcggcagccg gtcatgcact cgccacaccc cgtgcaggta tgccgtcgcg gcccttcgcc
     1381 accgaagtag gggtcgtcga cgggctggcc cttgctctcg ccgtcgcgcg ggaagaggac
     1441 cccgaccggg gtgtagcgga aggtgtcgcc cttgcccatc tcctcggcga cctggcggat
     1501 cacctcgtcc gagggcgtgt gccacgggtt ggtgatgacg ccgagcatcc gggtggcctg
     1561 gtcgtagtag ggcgccaact cggtcttcca gtcggtgatg tcgcgccact gtgggtcgtc
     1621 gtagaaggcg gccggaggct ggtaaagggt gtttgcgtag acgagcgagc caccgccgac
     1681 cccggctccg ctgaggatga ggcagtcgtt gagcaggtcg atccgctgga tcccgtagca
     1741 gcccaacgac ggcgcgaaca gatagttgcg cacatcgaaa ctcgtgtcgg gcaggtcggc
     1801 gtcctcgaaa cgtgggcccg cttcgaggac ggcgaccgag tagcccttct cggtcaggcg
     1861 tagcgctgcg accgatcctc cgaagcccga gccgacgatc acgacgtcgt aatcgaaggc
     1921 ggtggtcact taggccctcc cgaggcgggt gaggagcttg accgctccgg tcatgacggc
     1981 ggcatacgcc tcgtcggtca ggccccagtg cggcccgaag cggagcaggt attgcgtggc
     2041 gaccgactgg acctcggtgt agcggtggat gccctcggca ccctggcgcc gaccgatgcc
     2101 agaggagcgc atcccgccca taggggcgtc gaggctggcg aaggtcgcgc tgaaggcctc
     2161 gttgatgttg acggtgccgc acttgatccg gttggcgatg aggcgggccc gcttgttgtc
     2221 gcggctgaag accgcggcgt tgaggccgta gtcgccggcg ttggccaggt ccaccgcctc
     2281 atcctcggtg gtgaacgggt agaccgagat gaccggaccg aacgtctcgt cgg
//
LOCUS       DAOMZY010000437         2326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_47570_length_2326_cov_5.103919, whole genome shotgun sequence.
ACCESSION   DAOMZY010000437 DAOMZY010000000
VERSION     DAOMZY010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47570_length_2326_cov_5.103919"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="PLK64_16635"
     CDS             <1..1077
                     /locus_tag="PLK64_16635"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HPV81421.1"
                     /translation="YAVSRALVQAHDLGGTMTQPASSSASAPVVPHTMRAAVQDRYGS
                     PETVLEIREVPVPVLGTGEVLVKVAAASVNALDWHFTTGLPMFARASLGLRRPRRTTP
                     GVDVAGTVVAVGPDVSRWQPGDEVFGQVRGGGFAEFVAAPADSLVHRPDEVDVRDAAT
                     LGVAAQTALQGLRDWGQLQPGQRVLINGGSGGVGTFAVQLAKILGAGHVTAVCSTANV
                     ETARRLGADTVVDYLVEDHSATSERYDLFFDNAGTWSLGRCRRVLRPGGTYVMVTAPK
                     SPWLHPLPRLLAAPAAFLFSGRRAVAGRTAQHLPADVELLRDLVSSGALRPVMDRQYR
                     LDDVGEALEQQGRFHARGKSLILP"
     gene            1370..>2326
                     /locus_tag="PLK64_16640"
     CDS             1370..>2326
                     /locus_tag="PLK64_16640"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPV81422.1"
                     /translation="MDELLADRYERRGLLGRGAMGDVWLAYDARLGRLVAVKQLRRAL
                     ELDAHGVDRLIREARLAATLQHARVVAVYDLLIVDEQPIVVMEYVEGESLATLLRRTK
                     GVDVATALVLGRDVGLALAAAHEAGIVHRDVKPANILIAANGRAKLADFGIARGSDDS
                     ALTGTGQMVGTVAFMAPETALGSPPTPAADIYSLGATLYSAVEGHAPFTSEDGRQNTA
                     TMLLRMIREDAPTPRRAGPLSPLLSRMLSREVTNRPTAAEVLTILTDLIRAPDELASS
                     PAASGPSAAPGPRSMDSAAAAVVGVARAPIHQPGADDAVSAND"
BASE COUNT          347 a          782 c          796 g          401 t
ORIGIN      
        1 tacgccgtaa gtagagcgct cgtccaggct cacgacctcg gaggcaccat gacccagccc
       61 gccagcagtt ccgcttcagc gcccgtcgtc ccgcacacga tgcgggccgc ggtgcaggac
      121 cggtacggct ccccggagac cgtgttggag atccgggagg ttcccgtgcc cgtgctgggc
      181 acgggtgagg tcctggtcaa ggtggctgcg gcgtccgtca acgccctcga ctggcatttc
      241 acgacagggc tgcccatgtt cgcgcgggcc agcctcggcc tgcggcgacc gcggcgcacc
      301 acgccgggcg ttgatgtcgc cggcacggtc gtcgcggtgg gcccggacgt gagccggtgg
      361 cagccgggtg acgaggtgtt cggccaggtg cgcggcggcg gcttcgccga gttcgtcgcg
      421 gcaccggccg actcgctggt ccatcgaccc gacgaagtcg acgtgcgcga cgccgcgacc
      481 ttgggggtag ccgcccagac agcgttgcag gggctgcggg actggggcca attgcagcct
      541 ggtcaacggg tgctgatcaa cggcgggtcc ggcggcgtcg gcaccttcgc cgtgcaactg
      601 gccaagatcc tcggcgccgg tcacgtcacc gcggtatgca gcacggccaa cgtcgagacc
      661 gctcggcggc tgggcgcaga caccgtcgtc gactacctgg ttgaggacca ctcggcgacc
      721 tccgagcgct acgacctctt cttcgacaac gccggcactt ggagcctggg tcggtgtcgc
      781 cgcgtcctgc ggcccggcgg cacctacgtc atggtcacgg caccgaagtc gccctggctg
      841 caccccctgc cgcggctcct cgccgccccc gccgcgttcc tcttctcggg ccggcgagct
      901 gtcgccggac gaacggcaca gcacctcccg gccgacgtgg aactgttgcg tgacctcgtg
      961 tccagcggtg ctctgcgacc agtcatggac cggcaatacc gcctcgacga cgtcggcgag
     1021 gcgctggagc agcagggccg gttccacgca cgcggcaaga gcctcatcct gccctgagcg
     1081 acagccaccg gctcgaatca ggacccgcgg gtgcccccgg gcccgccccg cccctccgct
     1141 tggacgacct tagtcacgct tgtatgccgt tggtgcctca ttcaagcgtc actaacctca
     1201 tgcatgccgg acggtctgtc ggtggcgccg cgccgccagg ctgtttcacc agaggggcag
     1261 cggcgcaagg ttgggaggat cctccgcaga tcgcttgctt ggatcatgct tccctgaacc
     1321 agtacggtag ggctgtttca gccgacaata agaatttcgg tgatcccgca tggatgagct
     1381 tcttgccgat cgatacgagc gaaggggcct tctcggccgg ggcgcgatgg gagacgtttg
     1441 gcttgcgtat gatgcgcgat tggggcgcct cgttgcggtc aagcagcttc gtcgggcgtt
     1501 ggagttggac gcccatggcg ttgaccgtct gattcgagag gcccggctgg ccgcgacgct
     1561 gcagcacgcc cgagtcgtcg cggtttatga cctgctcatc gtcgatgagc agccgatcgt
     1621 ggttatggag tacgtcgagg gcgagagctt ggcaactctg ctgcgccgca ccaagggtgt
     1681 cgacgtggcg acggcgttgg tgcttgggcg cgatgtgggt ttggccttgg ccgcagcgca
     1741 cgaggcgggg attgtgcatc gtgacgtcaa accggcgaat atcttgatcg ccgctaacgg
     1801 ccgtgccaaa ctcgccgact tcggcatagc taggggctcg gacgactccg cgctgaccgg
     1861 gacgggtcag atggtcggaa cggttgcctt catggctccc gagacggcat tgggtagccc
     1921 gcccaccccg gctgcagaca tctactcctt gggcgcgacg ctgtattcgg ctgtcgaggg
     1981 gcatgcgccc ttcaccagcg aagacgggcg gcagaacacg gcaaccatgt tgctgcggat
     2041 gatccgcgag gacgctccga cgccacggcg agctggcccc ctgtctcctt tgctgagccg
     2101 aatgctgtcc cgcgaggtga ccaatcgtcc aactgccgcg gaagtgctca cgatcctgac
     2161 tgacctgatc cgggctccgg atgaactggc cagcagccct gcagcctccg gcccttccgc
     2221 cgccccgggg ccgcggtcca tggactcggc tgcggcagcg gtcgttgggg tggctcgggc
     2281 acctatccat cagcccggcg cagacgacgc agtcagcgca aatgat
//
LOCUS       DAOMZY010000438         2311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_48049_length_2311_cov_8.575798, whole genome shotgun sequence.
ACCESSION   DAOMZY010000438 DAOMZY010000000
VERSION     DAOMZY010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48049_length_2311_cov_8.575798"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="PLK64_16645"
     CDS             <1..851
                     /locus_tag="PLK64_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPV81423.1"
                     /translation="DTLAMVSAERVRDEFTKLLMSASPRAGLTLLVDSGLAQVFIPEL
                     PALRLELDEHHRHKDVYEHSLIVLEQAIALEGRTNPADAAYPVVTGPDLVLRLSALLH
                     DIGKPATRTFEGGGGVSFHHHELVGARLVAKRLRAMRFDKAQVGDVARLVELHLRFHG
                     YGEGQWTDSAVRRYVTDAGPLLGRLHLLTRSDCTTRNVRKATRLARTYDELEGRIARL
                     AAEEELAAIRPELNGNEIAAVLGIAPGPVLGEAYKFLLGVRLDEGPIGPEAAAARLRE
                     WWAARG"
     gene            1030..1203
                     /locus_tag="PLK64_16650"
     CDS             1030..1203
                     /locus_tag="PLK64_16650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81424.1"
                     /translation="MTGTGFFSGSAAKHIEKRLGRLGVTVLARSSFLVAATPGPLADG
                     ELARAEAWGASLG"
     gene            1343..2086
                     /locus_tag="PLK64_16655"
     CDS             1343..2086
                     /locus_tag="PLK64_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HPV81425.1"
                     /translation="MATDVGRVREHNEDAAFARGSIFVVADGMGGHAAGEVASAIAAS
                     TLGELAERADLTVADVVAQVDVANRRIFTTAVRNPQQWGMATTLTGVALVLDPDADPD
                     ADPEADPGSSWAVVNLGDSRVYRYAAGELAQVSVDHSEVQEMVDAGYLTDDEARVHPL
                     RHTVTRSLGRDRLPVVDTWVLPVVAGERFVVCSDGLTNEVRDAEIAAVLAVGADVQAT
                     AQDLVDRAVAHGGRDNVTVIVVEAADGPA"
BASE COUNT          321 a          751 c          866 g          373 t
ORIGIN      
        1 ccgacaccct cgccatggtc tcggcggagc gggtgcgcga cgagttcacc aagctcctca
       61 tgtcggcctc gccgcgggct gggctgaccc tgctcgtcga ctccgggctg gctcaggtct
      121 tcatccctga gctgcctgcg ctgcggctgg agctcgacga gcatcaccgg cacaaggacg
      181 tctacgagca ctcgctcatc gtccttgagc aggcgatcgc gctggaggga cggacgaacc
      241 cggcggacgc ggcatacccc gtggtgaccg ggcccgacct cgtgctgcgc ctgtccgcgc
      301 tgctgcacga catcggcaag ccggcgacgc gcaccttcga gggcggcggt ggggtgtcgt
      361 tccaccacca cgagttggtc ggcgcgcggc tggtcgccaa gcggctgcgg gcgatgcggt
      421 tcgacaaggc gcaggtcggt gacgtggcgc ggctggtcga gttgcacctg cgcttccatg
      481 gctatggcga ggggcagtgg accgactcgg cggtgcggcg gtatgtcacc gacgcggggc
      541 cgctgctggg gcggctgcac ctgctgaccc gatcggactg tacgacccgc aacgtccgca
      601 aggcgacccg gttggcgcgc acctacgacg agttggaggg gcggatcgcc cggctcgcgg
      661 ccgaggagga gctcgcggcg attcggccgg agctcaacgg caacgagatc gcggcggtgc
      721 tggggatcgc gccggggccg gtgctgggtg aggcctacaa gttcctgctc ggcgtgcggc
      781 tggacgaggg gccgatcggc cccgaggctg cggccgcacg gttgcgcgag tggtgggccg
      841 cgcgcggctg agcgttgggc gctgccgaaa tatcgtcgag ctttcgttgg gtcaacgaca
      901 tttcatcgag gccccagaac atcgtcgacc tttttgtgtc ctgggcggtg gcgttcagct
      961 gcgggggttg tcgagtggct ggatgcgctg ccgcgatccc tcgttgatca ggcggccgcc
     1021 ttcgacacgg tgaccggcac cgggttcttc tccggctcgg cggccaaaca catcgagaag
     1081 cgccttggcc gactcggcgt caccgtcctc gcccgaagca gcttcctcgt cgccgcgacc
     1141 cccggcccgc tcgccgacgg tgagttggct cgggccgaag cttggggcgc ctcgctgggc
     1201 tgaccggcat actcccggcg ttggcccagc cacccgcagg gcctccttcg cgggagcgag
     1261 cgcggaggtg acggaacggc ataccctctg acgaaggcga tgacgggagg cggcgaggat
     1321 ggcgctggag gtgcgcgtag gcgtggcgac cgacgttggc cgggtgcgcg agcacaacga
     1381 ggacgcggcg ttcgcgcgcg ggtcgatctt cgtcgtcgcc gacgggatgg gtgggcacgc
     1441 ggccggcgag gtggccagcg cgattgccgc atccacgctc ggggagttgg ccgagcgcgc
     1501 cgatttgacg gtggccgatg tcgtcgcgca ggtcgatgtg gcgaaccggc ggatcttcac
     1561 gacggccgtg cgcaacccgc agcaatgggg gatggcgacg acgctgaccg gagtggccct
     1621 cgtgctcgac cccgacgccg accccgacgc cgaccccgaa gccgacccag gcagcagttg
     1681 ggcggtcgtc aacctcggtg actcgcgggt ctatcggtat gccgcgggcg agctcgccca
     1741 ggtgtccgtc gaccactcgg aggtgcagga gatggtcgac gcgggttacc tgaccgacga
     1801 tgaggcgagg gtgcatccgt tgcggcacac cgtgactcgc tcgttgggtc gggatcggct
     1861 tccggtcgtc gacacgtggg tgctgcccgt ggtggccggt gaacgcttcg tcgtctgctc
     1921 cgacgggctg acgaacgaag tgcgtgacgc cgagatcgcg gcggtgttgg ccgtgggtgc
     1981 tgacgtccag gcgaccgcgc aggacctggt cgatcgggcg gttgcccatg gcggccgcga
     2041 caacgtgaca gtcatcgtgg tcgaggcggc tgacgggccc gcgtgaggcc gcgtagcgcg
     2101 gcatacctcg gcgtgggtat gccgcccgct cgccgtccgc gcgcgacccc cgtaacgatc
     2161 gtgtggcccc atcaccccca gagggtgaca gggccacaca atgtctaaag aactagatcg
     2221 tcagcgctat tgctgaacgg tcgcgcttcc tcagctcggg ctcgtacctc gcccgctaga
     2281 tcgtcagcgc tcttcggtgc cggcgatgaa g
//
LOCUS       DAOMZY010000439         2237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_50532_length_2237_cov_5.470211, whole genome shotgun sequence.
ACCESSION   DAOMZY010000439 DAOMZY010000000
VERSION     DAOMZY010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50532_length_2237_cov_5.470211"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PLK64_16660"
     CDS             complement(<1..359)
                     /locus_tag="PLK64_16660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPV81426.1"
                     /translation="MAPKPQAPPPDDKDWTWVLTQPCQECGFDPATIRSKRLADAVRE
                     TASRWPAVLVQPAVAERPSKAVWSPLEYACHVRDVMRLFAQRIELMLAEDNPTFANWD
                     QDRTAREDRYWDQDPAKV"
     gene            438..1538
                     /locus_tag="PLK64_16665"
     CDS             438..1538
                     /locus_tag="PLK64_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HPV81427.1"
                     /translation="MPENLLWWIVGAVVLFAGIAVAAAMVNQGVVDYDALTQRLRRQR
                     PHRDSFRDTPVVSDEPPRRAAVIVNPTKVDDLSEVRARIAGVCAAHGWGPPIWAETTA
                     EEPGTAQARQAVLDGAVLVCPLGGDGTVRAVAKALVDTETPLGLLPGGTGNLLARNLD
                     LPLDSVEKALVVALTGHNKSVDVGQIIIDRGNGAEPEEDTFLVMAGIGFDAAVMAGAP
                     EHLKRQVGNAAYILAGLRNMKGPQFKARMSVDGGMDFTRRTRTVLLGNCGKIFGGLVL
                     MPEAKIDDGLIDAVAMSPKGIVGWTAVLARIVSRQRKGHPIVDHHTGREIRVRMDRPE
                     ELQLDGDTLGPVRSVTASVRSGALVVRVAGSG"
     gene            complement(1535..>2237)
                     /locus_tag="PLK64_16670"
     CDS             complement(1535..>2237)
                     /locus_tag="PLK64_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HPV81428.1"
                     /translation="GAVLTFGTSGLDDRTAELVGGLASLLAVVLVTGMIFWMRSVGRR
                     FAGELTGKLDRALDAGPFAIAAVAFLGVGREGLESALFFYATVESAGETGIAPALGWV
                     VGIGAAVALGIAIYLGAVRIDLAQFFRWTGVGLVIVAAGILAYAVHELQEAGVLPGEN
                     VYAFDLRSTIDPTSPLAMVVRGILNLRPRMAVLEIGAWAAYLVVVLPLFLRGVGRRPT
                     ATAAAAAESVPAAKR"
BASE COUNT          355 a          788 c          795 g          299 t
ORIGIN      
        1 accttcgccg ggtcctggtc ccagtagcgg tcctcgcggg ccgtgcggtc ctggtcccag
       61 ttggcgaacg tcgggttgtc ctcggcgagc atcaactcga tccgctgggc gaacaagcgc
      121 atgacgtcgc ggacgtggca ggcgtactcc agcggcgacc acaccgcctt ggacggacgc
      181 tcggcgacgg ctggctgcac gagcaccgcc ggccagcgtg aggcggtctc ccggaccgcg
      241 tcggccaggc gcttggagcg gatggtcgcc gggtcgaagc cacactcctg gcagggctgg
      301 gtgagcaccc aggtccagtc cttgtcgtcg ggaggcggcg cttggggttt cggggccatg
      361 catgaagtat gccgtcaagc cccgtcaggc cgcggggtcg agcgagtcga gttggtggcg
      421 tcgtatacgg tcggtgggtg cccgagaacc tgctgtggtg gatcgtcgga gcggtggtgc
      481 tcttcgcggg catcgccgtt gctgcggcca tggtcaacca gggcgtggtc gactacgacg
      541 cgctcaccca gcgcctgcgg cgccagcgcc cccatcgcga cagcttccgg gacaccccgg
      601 tcgtgtccga cgagccgccc cgccgggccg ccgtgatcgt caacccgacc aaggtcgatg
      661 acctcagcga ggtccgcgcg aggatcgccg gcgtctgcgc tgcccacggc tggggtccgc
      721 ccatctgggc cgagacgacg gcagaggagc cgggcaccgc tcaagcccgc caggccgtcc
      781 tcgacggcgc cgtcctggtc tgcccactcg gcggggacgg gacagttcgc gcggtggcca
      841 aggctctcgt cgacaccgag accccgctcg ggctgctgcc cggcggcaca ggcaacctgc
      901 tcgcccgcaa cctcgacctg ccgctggatt cggtggagaa ggcactcgtc gtggccctca
      961 ccggccacaa caagagcgtc gatgtgggcc agatcatcat cgatcgcggc aacggcgccg
     1021 agccggagga ggacaccttc ctcgtcatgg ccggcatcgg cttcgacgcg gcggtcatgg
     1081 cgggggcgcc ggagcacctc aagcggcagg tgggcaacgc ggcatacatc ctcgcgggcc
     1141 tgcgcaacat gaagggcccg cagttcaagg cgcgcatgag cgtcgacggt gggatggact
     1201 tcacccgacg cacccggacc gtgctgctgg gcaactgcgg caagatcttc ggaggcttgg
     1261 tgctcatgcc cgaggccaag atcgacgacg gccttatcga cgcggtcgcc atgtctccca
     1321 agggaatcgt cggctggacc gccgtgctcg cccgcatcgt cagccgccag cgcaagggtc
     1381 accccatcgt cgatcaccac accggtcgcg agatccgggt gcggatggat cggcccgaag
     1441 agctgcaact cgacggcgac accctcggcc cggtgcgatc ggtgaccgcg tcggtccgct
     1501 cgggtgcgct cgtcgtgcgg gtcgccggca gcggttagcg cttcgcggcc gggaccgact
     1561 cggctgcggc ggctgcggtc gccgtcggac gacggcccac cccgcgaagg aacagcggca
     1621 gcacgaccac gaggtatgcc gcccacgccc cgatctcgag cacggccatc cgtggccgca
     1681 ggttgaggat gccgcggacg accatggcca acggcgaggt cgggtcgatg gtcgagcgca
     1741 ggtcgaaggc gtagacgttc tcgcccggga gcacgccggc ctcctgcagt tcgtgcacgg
     1801 catacgcgag gatgccggcc gcgacgatca ccaggccgac gcccgtccag cggaagaact
     1861 gcgcgaggtc gatccgcacc gccccgaggt agatcgcaat gcccaaggcg accgcggcgc
     1921 cgatcccgac cacccagccg agcgcggggg cgatgccggt ctcaccggcg gactcgacgg
     1981 tggcatagaa gaagagcgcc gactccagcc cctcgcggcc aacgccgagg aaggccacgg
     2041 cggcgatcgc gaacgggccg gcgtcgaggg ctcggtcgag cttcccggtc aactcacccg
     2101 caaaccgccg ccccaccgag cgcatccaga agatcatgcc ggtgaccagg acaacggcca
     2161 gcagcgaggc cagcccaccc accagctcgg cggtgcgatc gtccagcccg ctggtgccga
     2221 aggtgaggac ggcgccg
//
LOCUS       DAOMZY010000440         2197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_52026_length_2197_cov_5.582166, whole genome shotgun sequence.
ACCESSION   DAOMZY010000440 DAOMZY010000000
VERSION     DAOMZY010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52026_length_2197_cov_5.582166"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="PLK64_16675"
     CDS             complement(<1..1131)
                     /locus_tag="PLK64_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPV81429.1"
                     /translation="MTTKRAIRPQWLIFILLAVGLTAFLMFFSDSGGYTRVDTSAALD
                     LIATDKVDTARVVNNERIELALKDGVTYSDGARVKDATKVYAFYVDARGPQIVKALDD
                     HPPAKGFTDQIDTQNWFASMLSLVLPMIIVLGLFWFLMGQMQGGGNRVMQFGKSRAKL
                     ASKDMPKVTFADVAGADEAVEELHEIKEFLQDPARFLAVGAKIPKGVLLYGAPGTGKT
                     LLARAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFEQAKANAPAIVFVDEIDAVG
                     RHRGAGLGGGHDEREQTLNQLLVEMDGFDVKTNVILIAATNRPDILDPALLRPGRFDR
                     QIAVEAPDMIGRHRILQVHSQGKPMAPDVDLLAVARRTPGFTG"
     gene            complement(1284..1835)
                     /gene="hpt"
                     /locus_tag="PLK64_16680"
     CDS             complement(1284..1835)
                     /gene="hpt"
                     /locus_tag="PLK64_16680"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HPV81430.1"
                     /translation="MDAPSMGSGLTRVLLTEEQILTRLDELATQIWADYAGKDLLLVG
                     VLKGAVLVMADLMRAMPGSVRMDWMAISSYGSGTKSSGVVRIIKDLDTDITNKHVLVV
                     EDIIDSGLTLSWIMANLSSRKPASLEICTLLRKPEVAKVKIDVKYVGFDIPNEFVVGY
                     GLDFAEDYRNLRCIGTLAPSVYS"
     gene            complement(1896..>2197)
                     /locus_tag="PLK64_16685"
     CDS             complement(1896..>2197)
                     /locus_tag="PLK64_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TilS substrate-binding domain-containing
                     protein"
                     /protein_id="HPV81431.1"
                     /translation="QAGAGDVPAVRVAVAGLAALPPALRRRVWRIVAAEAGARALSAA
                     HVALLDGLVLRWHGQGAIDLPGGVVALRVGGTIQMGPRPADVVARPVAQTSQPVR"
BASE COUNT          361 a          721 c          769 g          346 t
ORIGIN      
        1 gccggtgaag ccgggcgtgc gtcgggcgac ggccaacagg tcgacgtcgg gagccatcgg
       61 cttgccctgc gagtggacct gcaggatgcg gtggcgaccg atcatgtcgg gggcctcgac
      121 ggcgatctgg cggtcgaagc ggcctggacg cagcaacgcc gggtcgagga tgtcggggcg
      181 gttggtcgcc gcgatgagga tgacgttggt cttgacgtcg aagccgtcca tctcgaccag
      241 gagctggttg agggtctgct cacgctcgtc gtgcccgcca ccgaggccgg caccacggtg
      301 gcgaccgacg gcgtcgatct cgtcgacgaa gacgatggcc ggggcgttcg ccttggcctg
      361 ctcgaagagg tcccgcacgc gggaggcgcc gacaccgacg aacatctcga cgaagtccga
      421 gccggagatc gagtagaacg gaacgccggc ctcgccggcc acggcccgcg ccaggagggt
      481 cttgccggtg ccgggcgcgc cgtagagcag aacgcccttg gggatcttgg cgccgaccgc
      541 gaggaagcgg gccgggtctt ggaggaactc cttgatctcg tggagctcct cgacggcctc
      601 gtcggcaccg gccacgtcgg cgaaggtcac cttgggcatg tccttggagg ccagcttggc
      661 gcgcgacttg ccgaactgca tcacccggtt gccgccaccc tgcatctggc ccatgaggaa
      721 ccagaacagg ccgaggacga tgatcatcgg caggaccagg ctgagcatcg acgcaaacca
      781 gttctgggtg tcgatctggt cggtgaagcc cttggccggc ggatggtcat cgagtgcctt
      841 gacgatctgc ggaccacggg cgtcgacgta gaaggcgtag accttcgtcg cgtccttgac
      901 ccgggcgccg tcggagtagg tgacgccgtc cttgagcgcg agctcgatcc gttcgttgtt
      961 gacgacgcgg gcggtgtcga ccttgtccgt cgcgatgaga tccagggcgg cggacgtgtc
     1021 gacccgcgta taaccccccg agtctgagaa gaacatcagg aaggccgtca aaccgacggc
     1081 aagcaggatg aagatcagcc actgggggcg aatcgcgcgt ttcgtagtca tgcgtgtgcg
     1141 gggcgctgcc ccagcctcct ggtcgtcgtg cgtcgctcac cggcgctgcg gccaccacag
     1201 ggacgctcgc cgaaagggat tctcgcaggt caacgcccgg ctgtgcgccc gtgttccgcc
     1261 gtcacgcgat cactgtcacg cgatcagctg tagacggaag gagccagggt gccgatgcag
     1321 cgcaggttgc ggtaatcctc ggcgaagtcc agcccgtagc cgacgacgaa ctcgttgggg
     1381 atgtcgaagc cgacgtactt gacgtcgatc ttcaccttgg caacttcggg cttgcgcagc
     1441 agcgtgcaga tctccagcga ggccggcttg cgggagctga ggttggccat gatccacgag
     1501 agggtcaggc cggagtcgat gatgtcctcg acgacgagga catgcttgtt ggtgatgtcg
     1561 gtgtcgaggt ccttgatgat gcgcacgaca cccgaggact tcgtgcccga gccgtagctc
     1621 gagatggcca tccagtccat ccggaccgat ccgggcatcg cccgcatgag gtcagccatg
     1681 acgagcacgg cccccttgag cacgccgacg agcaacaggt ccttgcccgc gtagtcggcc
     1741 cagatctgcg tggccaactc gtcgagccgg gtgaggatct gctcctcggt gaggaggacc
     1801 ctcgtcagtc ccgaccccat gctcggtgcg tccacgacgt agtcctcctg gcgttagccg
     1861 gcctcgtggg ccgtccggtc gcggtcgagt cgaccctatc gaaccggttg cgatgtctgg
     1921 gcaacgggac gagccaccac gtctgcgggg cggggtccca tctgaatggt cccacccacc
     1981 cgcagcgcga ccacgccacc cggcaggtca atcgcccctt gcccgtgcca ccgcaggacc
     2041 agaccgtcga gcagggcgac gtgggcggcc gacagcgccc gcgccccagc ttccgcggcg
     2101 acgatccgcc agacccgccg gcgcagcgcg gggggcaggg cggccaggcc cgccaccgct
     2161 acccgaacgg ccggcacgtc cccggcgccc gcctgcg
//
LOCUS       DAOMZY010000441         2186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_52417_length_2186_cov_5.617081, whole genome shotgun sequence.
ACCESSION   DAOMZY010000441 DAOMZY010000000
VERSION     DAOMZY010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52417_length_2186_cov_5.617081"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PLK64_16690"
     CDS             <1..709
                     /locus_tag="PLK64_16690"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HPV81432.1"
                     /translation="LQADDRAAAGRRFASGLAVPAVVTIPGSIALVVFALPIMGLLFT
                     SKDPAEVLAGSTVLACLGPALFPMGIALLGRRVCEAVGAHWSAGALTAVLALTSIGVS
                     VGAGFAAPEWATPLVALGLVLGNTLAAAYGVWVVRRRIGTVALAGVVRTWVRILLAGA
                     GGGYVAWGLVMVLTRALGGLGRLGYALTLLIGGTAFIVVYLLVARLLRVDEVSVVAAP
                     LVNRLDHRPPGRHRPGD"
     gene            complement(844..1860)
                     /locus_tag="PLK64_16695"
     CDS             complement(844..1860)
                     /locus_tag="PLK64_16695"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009760582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HPV81433.1"
                     /translation="MPPLASSVGLPPLGELLDGLVVVRISLRVRFRGVIEREVALLRG
                     PRGWGEFGPFLEYGPRESSRWLACAIEAAYAGWPAPVRSAVPVNATVPAVAADSVAGV
                     LARFPGCTTAKVKVAEAGQGLADDLDRVAAVREILGPDGRIRVDANGGWDVASAQRAL
                     GALAAYRLEYAEQPCAEVSDLVVLRTLLARNGIDVPIAADESIRKAADPMRVAREGAA
                     DLIVVKVAPLGGVRRALEIVGDCGLPAVVSSALDTSVGMAAGVALAAALPELPWACGL
                     GTLALLEGDVVVDPAVQHGGVLSVSAEPPQVDEALVARWSAEPERVAWWTERVAAAYA
                     HLLA"
     gene            1960..>2186
                     /locus_tag="PLK64_16700"
     CDS             1960..>2186
                     /locus_tag="PLK64_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81434.1"
                     /translation="MSTGALPFVSATFAADAWAPVAGFELTDITYHRCVLPGPARGTV
                     RIAFDRPEVLNAFRPHTVDELYRVLDHARMTP"
BASE COUNT          307 a          877 c          703 g          299 t
ORIGIN      
        1 actgcaggcc gacgaccggg ccgccgccgg ccgccgtttc gcctcgggtc tcgcggtgcc
       61 tgcggtcgtc accatccccg gctcgatcgc cctcgtggtc ttcgccctgc cgatcatggg
      121 gctgctgttc acgtcgaagg acccggcgga ggttctcgct ggatccaccg tcctggcctg
      181 cctaggtccg gcgctgttcc ccatgggcat cgccctgctg ggccggcgcg tctgcgaagc
      241 cgtgggtgcg cactggtcgg ccggggctct gacggccgtg ctcgcactga cctcgatcgg
      301 cgtcagcgtc ggggccgggt tcgctgcgcc agagtgggcg acgcccctgg tggcgctcgg
      361 gctcgtgctc ggcaataccc tcgccgcggc atacggggtc tgggtcgtcc gccgccggat
      421 cgggacggtc gctctggcgg gcgtcgtccg cacctgggtg cggatcctcc tggccggcgc
      481 gggcggcggt tatgtggcct gggggttggt catggtcctc acccgggcgc tgggcgggct
      541 gggccgcctc gggtatgcct tgacgctcct catcggaggc accgccttca tcgtcgtcta
      601 cctgttggtg gcccgcctgc tgcgggtcga cgaggtgtca gtcgttgctg cacccctggt
      661 caaccggctc gatcatcggc cccccggccg gcatcgcccc ggcgactgat ccgccgggga
      721 ccgcccctcg cagctgaggg cgcgacggag tgcgatcgac ggggtgatca ccccatcaaa
      781 cgcacaccgt cccgcactcg actcgctggc gagagatagc cagcccgtcg ctacccagcc
      841 gactcaggcc agcaggtgcg cgtatgccgc cgccacccgt tcggtccacc acgccacccg
      901 ctccggctcg gccgaccacc gcgccaccag ggcttcatca acctgcggcg gctcggctga
      961 caccgacagc actcccccgt gctgcaccgc cggatcaacc acgacgtccc cctcaagcaa
     1021 cgccagagtc ccgagcccac acgcccaggg cagttcgggc aacgcggcag ccaacgccac
     1081 cccggccgcc atgcccaccg acgtgtccag cgccgacgac accaccgcgg gcagcccaca
     1141 atcacccacg atctccagcg cccgacgcac cccgcccaac ggcgcgacct tgaccacgat
     1201 gaggtccgcc gccccctccc gagccacccg catcgggtcg gccgccttgc gaatcgactc
     1261 gtcagcggcg atgggcacgt cgattccgtt acgcgccaac aaagtccgca acaccacgag
     1321 atcagagacc tccgcgcacg gctgctcggc atactccagc cggtatgccg cgagcgcacc
     1381 gagcgcccgc tgcgcggacg ccacgtccca cccgccattc gcgtcgaccc ggatccggcc
     1441 gtccggcccg aggatctcgc gcaccgcggc cacccggtcg aggtcgtcgg ccagcccctg
     1501 gccagcctca gccaccttga ccttggccgt cgtgcacccg gggaatcgcg ccaacactcc
     1561 agccaccgag tcggccgcca ccgccggaac tgtcgcattg accggcaccg ccgagcggac
     1621 gggcgccggc cacccggcat acgccgcctc gatcgcgcag gccagccaac gggaggattc
     1681 gcgcgggccg tattccagga acgggccgaa ctcaccccac ccgcggggtc cgcgaagtag
     1741 cgccacctca cgctcaatga cgccgcggaa gcgcactcgc agggagatcc ggacgacgac
     1801 gagaccatca agcagctcgc ccagcggcgg caacccgacg gagctggcga gcggcggcat
     1861 acctgcgagt gtgccagccc gcgcctcccc cacgggaggc cgggccgccg cgatggactc
     1921 gacgggcggg gggtgcaagc gaggccatat ccttcggggg tgagcaccgg cgccctcccc
     1981 ttcgtctcgg cgaccttcgc cgccgacgcg tgggccccgg ttgcgggttt cgaactcacc
     2041 gacatcacct accaccggtg cgtgctcccc ggcccggctc gcggcaccgt ccgcatcgcc
     2101 ttcgaccggc ccgaggtgct caacgccttc cggccgcaca ccgtcgacga gctctaccgg
     2161 gtgctcgacc acgcgcggat gacccc
//
LOCUS       DAOMZY010000442         2145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_54021_length_2145_cov_8.037321, whole genome shotgun sequence.
ACCESSION   DAOMZY010000442 DAOMZY010000000
VERSION     DAOMZY010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54021_length_2145_cov_8.037321"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(374..1282)
                     /locus_tag="PLK64_16705"
     CDS             complement(374..1282)
                     /locus_tag="PLK64_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81435.1"
                     /translation="MAQPLRLATKRREFRSLTSPDRYQDLLSELGVGAWLVDHGVAFE
                     FGKTGGQPQPDLVLPDFGFGIEVTRRKRSPDADLRRAISKGVYSAIPGAERHEWKRSG
                     KRIPTPVARVTGQPIAIRRRVLDDLSVEVEQAIRQGHQHVHGVVRPARDGYPAVVASI
                     DLYGGRSGMPRIVHDERWLDLTIPMRDIEDLVLAALEDRRKSVQGSSMPTILLIDASQ
                     LSQLLWLRSLSTWADRLGMLLSPDHTFVGVGLTKLWQGPRLGLGVGPRATPETRDSVT
                     RWADQMGMRLTTPNLPNRVPRKGDAE"
BASE COUNT          385 a          702 c          672 g          386 t
ORIGIN      
        1 ccatggtccc cgtgacggac ccgctggcgt gttactgacc cacgctccta ggctggcatc
       61 gtgctagcgg gtaagaccgt gaccttgcgc caggtccgtg aagtggacct ggacgcgctg
      121 tacgacgcgc acacaaacat ccgtaaccgg ggtgcgtact tccctcttgg cgtcatgtcc
      181 cagacggcct ttcgtggcga gttcggtgag cacggcttgt ggcagaagag cgagggatgc
      241 tcgtcatcga gtccccagtg ggcgagatcg ccggtcacat cgagttcttc cgcccggtct
      301 cctactggga cgcgttcgag ctgtcgctcg cgtagacaag agcttcctag cgtggcggta
      361 gtgcgcgcgg cagtcactcg gcgtcgccct tgcgcggcac tcggttcggc aggttggggg
      421 tcgtcaagcg catacccatc tggtccgccc agcgggtcac gctgtcccgc gtctctggcg
      481 tcgcccgagg gccgacgcca agccccagtc gaggcccctg ccacagcttt gtcaggccga
      541 ctccgacaaa cgtgtgatcc ggcgacaaca acatgcccag ccggtcagcc catgtggaaa
      601 gggagcggag ccacaacagt tgcgacagct gagacgcgtc aatgaggaga atggtcggca
      661 ttgagcttcc ctgcaccgac tttcgacggt cctccagagc cgccaagacc aggtcctcga
      721 tgtcacgcat agggattgtg aggtccaacc acctctcgtc gtgaacgatt cggggcatcc
      781 cggaacggcc cccgtagaga tcgatgctcg ccacgaccgc gggatatccg tctcgggcgg
      841 ggcggacgac gccgtgaaca tgctggtggc cctggcggat cgcctgttca acctcgacac
      901 tcaggtcgtc caacacccgt ctgcggatgg cgatcggctg ccctgtgacc ctcgcgaccg
      961 gagtgggtat ccgtttgccc gagcgtttcc actcgtgccg ctcggccccg gggattgccg
     1021 agtagacacc ctttgaaatg gcccgccgca ggtcggcatc ggggctgcgc ttgcggcggg
     1081 tgacctcaat cccgaagccg aagtccggca gcaccagatc gggctgcggc tggccgccgg
     1141 tcttgccgaa ctcgaacgcc acgccgtggt cgaccaacca ggccccgaca cccagctcgc
     1201 tgagcaggtc ctggtagcgg tcagggctcg tcagcgaccg gaactcccgc ctcttagttg
     1261 ctaggcgaag gggttgcgcc atcaccagtc gatggagaag gtcctctacg cgctgccagt
     1321 ccatgggacg ggatgtgtct gtcagcgcac ccgccaaggg gtggttaggc ctgcacccga
     1381 acatccactc ctcgaggatc gtcggcaagg ccatggatcg tttctacctg accgacacag
     1441 gttccacccg ggtcgaccga ctcgcggcta ctagacgaat gcccgtaggt ccggtgtctg
     1501 gccagactcg gtccaggtga tcggcgggag actctcttgc ggtttctcac cgaagtgacg
     1561 cggccccgtc ggaggagcga agcatcgcgc ggccaagtct acggcgtctc acgtattcgc
     1621 tgcagccgct cggtctcgag ttcagatggg cccgggaatc gacgcctaag gtccactact
     1681 tcgaggagtg cgcgggccag aacaacggcg ggtgataaca ccccgatggc cagggctatg
     1741 tctgcgcctc ctgacccgga ctgatgtgca gatatgtgtc gtaggacgcc ccgggcccaa
     1801 gggcgcccgt gcgcaactcc tgagtagatc ctccaagcgc gcagggtgtt ggttccaaga
     1861 gcgacatcta ccgcgttgac gatctcagtt gtggagactc gcgccctgat gtccaatccc
     1921 aaattggcaa ggagccgaac tccctcctta gggagcggcg agcgagcccc ctcatccgcc
     1981 ccaagaaggt cgctcatgtc ttgaaatccc aaggtgagat accgcttggc tcgctcgtcg
     2041 gattccttcg gtaagcacaa ccacaaacca gtggcgaggc actcgatggc tgaacgcgcc
     2101 atgacgacgc aagaactcac caagtccgcg ggctggtcct cctcc
//
LOCUS       DAOMZY010000443         2125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_54787_length_2125_cov_6.558937, whole genome shotgun sequence.
ACCESSION   DAOMZY010000443 DAOMZY010000000
VERSION     DAOMZY010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54787_length_2125_cov_6.558937"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="PLK64_16710"
     CDS             complement(<1..1071)
                     /locus_tag="PLK64_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HPV81436.1"
                     /translation="MTTALRYAARTHLGLGPKSRNEDSGYAGPHLLVLADGMGGHAAG
                     DVASSMIVGALAPLDEEIVSGEQALRVLESELREANAQLRAAMREDSGLAGMGATTIA
                     MLRSGNKLAMAHIGDSRAYLLRDGTFSQITKDHSFVQQLVDEKRITEAEAQHHPQRSL
                     VTRVMTGNPDDQPDLSIRELIPGDRYVLCSDGLTDFVSPDIVAEIITAAPTPEAAAER
                     CIEVALKASTRDNVTVVVADVVDSNELADLPSTPEIVGAAAVRGRARRKTRAIPVSPA
                     EKAAALTREVSGTPDDEPLQLAEEVASPRVLWLRRTAVGLAAAVLVVGAGYAGYDWTQ
                     RQYFLGAQDGYVTLYRGVNQTLG"
     gene            complement(1071..1643)
                     /locus_tag="PLK64_16715"
     CDS             complement(1071..1643)
                     /locus_tag="PLK64_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPV81437.1"
                     /translation="MSELTLTLMRLGLLVMLWLFVFAVVGVLRSDLFGTRVSRRTRRR
                     AAQTGVPVGAAPASGYAAPAPSPHGAPPARQAARPAPARQSGRGGAGARGIPTSVAVT
                     EGRLAGTVIPLTPAGILVGRNPECSLVLDDDFTSGRHLRLFLREGSWWVEDLGSTNGT
                     FLDAARLTTAQPVGVGSVLRIGRTALELHG"
     gene            complement(1648..>2125)
                     /locus_tag="PLK64_16720"
     CDS             complement(1648..>2125)
                     /locus_tag="PLK64_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPV81438.1"
                     /translation="LSHPAYDASSHVAAPDVELLPDRALAPDQPAVAPPPPPPLAAPP
                     PAPAPAPPAKLPRINPAQRPWLDIDGERYPLLGSLTVIGRDATADVILDDPGISRRHS
                     EVRVTHDGPHLVSSIRDLASTNGTFVNGERIASTRLIDGDQITVGRTAITFRAGKR"
BASE COUNT          281 a          678 c          858 g          308 t
ORIGIN      
        1 cccgagggtc tggttgaccc cgcggtagag ggtgacgtag ccgtcctggg cgccaaggaa
       61 atactgccgc tgggtccagt cgtaaccggc ataccctgcg ccgacaacga gcacagcggc
      121 agcgagcccg accgcggtgc ggcgcaacca cagcacgcgg ggcgaagcga cctcctcggc
      181 gagttgcagc ggctcgtcgt cgggggtgcc ggacacctcg cgggtgagtg cggcggcctt
      241 ctcggcgggg ctcacgggga tggcgcgggt cttgcgtcgg gcgcggccgc gcacggcggc
      301 ggcaccgacg atctcaggcg tgctgggcag gtcggccagc tcgttgctgt cgacgacgtc
      361 ggccaccacc acggtgacgt tgtcgcgggt gctggccttg agagcgacct cgatgcagcg
      421 ctcggcggct gcctcggggg tgggcgccgc ggtgatgatc tcggcgacga tgtcggggct
      481 gacgaagtcg gtgagcccgt cggagcacag gacgtaacgg tcgccgggga tgagctcgcg
      541 gatcgacagg tcgggctggt cgtcggggtt gccggtcatc acccgagtga cgagggagcg
      601 ctgagggtgg tgctgggcct cggcctcggt gatgcgcttc tcgtcgacga gttgctggac
      661 gaacgagtgg tccttggtga tctggctgaa ggtgccgtcg cgcagcaggt aggcccggga
      721 gtcgccgatg tgggccatgg ccagcttgtt gcccgagcgc agcatggcaa tcgtcgtggc
      781 gcccatgccg gccaggccgc tgtcctcgcg catcgccgcg cgcagttggg cattggcctc
      841 gcgcaactcg ctctcgagca cccgcaacgc ctgctcgccc gagacgatct cctcgtccaa
      901 cggggcgagg gccccgacga tcatcgagct ggcgacgtcg ccggcggcgt gcccacccat
      961 gccgtcggcg aggacgagca ggtggggacc ggcatacccg gagtcttcgt tgcggctctt
     1021 gggtccgagg ccgaggtggg tgcgcgcggc atagcgcagg gccgtcgtca tcagccgtgc
     1081 agctccagcg cggtgcgccc gatccgcagc acgctgccga cgcccaccgg ctgggccgtg
     1141 gtcagtcggg cggcgtcgag gaaggtgccg ttggtcgagc cgaggtcctc gacccaccag
     1201 gacccttcgc gcaggaacag ccgcaggtgt cgccccgacg tgaagtcgtc gtcgaggacc
     1261 agcgagcatt cggggttgcg cccgacgagg atccccgccg gggtgagcgg gatgaccgtc
     1321 cccgcgagcc gaccttcggt gacggccacg cttgtcggta tgccgcgtgc tcccgcgccg
     1381 ccgcgaccgc tctggcgggc cggcgccgga cgggcggcct gccgagcagg gggtgcgccg
     1441 tgcggcgagg gtgcgggggc ggcatacccg gaggcgggtg ctgcgccgac ggggacgccg
     1501 gtctgggcgg cccggcgtcg ggtgcggcgg gagactcggg tgccgaacag gtcgctacgc
     1561 agcaccccga cgaccgcgaa gacgaacagc cacaacatga cgagcaaccc caaccgcatg
     1621 agggtgaggg tgagttcgct caccggctca cctcttcccg gcgcggaacg tgatggcggt
     1681 ccgcccgacg gtgatctggt cgccgtcgat gagccgggtg ctggcgatcc gctcgccgtt
     1741 gacgaaggtg ccgttggtcg acgcgaggtc gcgaatgctc gacacgaggt gcgggccgtc
     1801 gtgcgtgacg cgaacttcgc tgtgtcgccg cgagattccc gggtcgtcga ggatgacatc
     1861 ggcggtcgcg tcgcggccga tgacggtgag ggagcccagc agggggtagc gctcgccgtc
     1921 gatgtcgagc caggggcgct gggccgggtt gatccggggc agcttggcgg ggggcgccgg
     1981 cgctggggct ggtggtggcg ccgcgagcgg gggtggcggc gggggcgcga ccgcgggctg
     2041 gtcgggtgcg agagcccggt cggggagcaa ctcgacatcc ggggcggcca cgtggctgga
     2101 ggcgtcgtat gccgggtggc tcagc
//
LOCUS       DAOMZY010000444         2114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_55305_length_2114_cov_3.949004, whole genome shotgun sequence.
ACCESSION   DAOMZY010000444 DAOMZY010000000
VERSION     DAOMZY010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55305_length_2114_cov_3.949004"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PLK64_16725"
     CDS             <1..583
                     /locus_tag="PLK64_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010534287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HPV81439.1"
                     /translation="IDVIGHELAHGLTQYTAGLTYVAQSGALNESVSDVFGILVKQMH
                     ENETAETSDWLIGEGLFTDRVKGVALRSMKAPGTAYDDPVLGRDPQPAHMDDFEDLPH
                     DAEHDNGGVHTNSGIPNRAFYLAASALGGYAWERAGQIWFDTITAKGVPKDIDFAGFA
                     ARTVAAASKRYGTTSEEVTAVEEAWATVGVELP"
     gene            complement(590..1015)
                     /locus_tag="PLK64_16730"
     CDS             complement(590..1015)
                     /locus_tag="PLK64_16730"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPV81440.1"
                     /translation="MTLVVLDTNVISEAMRGAAASPQVIAWLRSLAATPITTVVNRAE
                     ILAGIALLAPGRRRTDLRAAAESAFDGLGVCLPLTSVAAEHYAQVVAHRRDAGRPIGG
                     MDALIAAITRQNGAALATRDIAGFAELGIDLIDPWAYRA"
     gene            complement(1012..1260)
                     /locus_tag="PLK64_16735"
     CDS             complement(1012..1260)
                     /locus_tag="PLK64_16735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81441.1"
                     /translation="MTTAITIRQLPLAAKQQLRMRAATNGRSMEAEARTILLEALDRS
                     PDADLTWIEQLMAAGQRAGGVDLPEIDDEPATAADFGA"
     gene            complement(1303..2106)
                     /locus_tag="PLK64_16740"
     CDS             complement(1303..2106)
                     /locus_tag="PLK64_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPV81442.1"
                     /translation="MSTLVFVHAHPDDEASQTAGAMCRAVEEGHRVVLVVSTGGEHGE
                     AAADLAPGETVAQRRRAELAGSAAALGVHRVEWLGYVDSGMTGWDQNDHEHAFVQAPL
                     DEAAGRLAAILDDESADVLIGYDWHGGYGHPDHVQVHKVVHRAAELAALRPRLLEATM
                     NRDIMRSFVAAARAAGMDDGENGFDPDGPMDDGNPMGSPEAEIHWQVDVAAYLPQRRA
                     ALSCHASQTTDVGGMLSMPEQVFAGFFGREHYIEPGRPAGMAQAWFLDA"
BASE COUNT          353 a          734 c          710 g          317 t
ORIGIN      
        1 catcgacgtc atcgggcacg agctcgccca tgggctcacg caatacacgg cggggctcac
       61 ttatgtcgcc cagtccggtg cgcttaacga gtcggtgtcc gacgtgttcg gaatcctggt
      121 caagcagatg cacgagaacg agaccgccga gacctccgac tggctcatcg gcgagggcct
      181 gttcaccgac cgggtcaagg gcgtcgcgct gcgctcgatg aaggcgccgg gcacggcata
      241 cgacgacccg gtcctcggcc gcgatccgca gccggctcac atggacgact tcgaggatct
      301 gccccacgac gccgagcacg acaacggcgg tgtccacacc aattccggta tccccaaccg
      361 ggcgttctac ctcgcggcga gcgcgctggg tggctacgcc tgggagcggg ccgggcagat
      421 ctggttcgac acgatcacgg ccaagggggt gcccaaggac atcgacttcg cgggcttcgc
      481 agcgcgcacg gtggcggcgg cgagcaagcg ctacggcacg acgagcgaag aggtcaccgc
      541 ggtcgaggag gcctgggcca ccgtcggcgt cgaactcccc tgagcagggt tacgcgcggt
      601 aggcccacgg atcgatgagg tctatcccca actcggcgaa cccggcgata tcccgcgtgg
      661 ccagcgctgc gccgttctgc cgggtgatgg cggcgatcaa cgcgtccatg ccgccgatcg
      721 gacgtccggc gtcacgccga tgagcgacaa cttgggcata gtgctcggct gctaccgacg
      781 tgaggggcag gcacaccccc aggccgtcga acgccgactc ggcagcggca cgcaagtccg
      841 ttcgacgccg gccaggggcc agcagggcaa tgccggccag gatctcggcc cggttcacca
      901 cggtggtgat gggggttgcc gccaaggacc gcagccacgc gatgacctgc ggactcgccg
      961 ccgctcctcg catggcttct gagatgacgt tcgtgtcgag gacgacgagg gtcatgcgcc
     1021 gaagtcagcc gcagttgcag gctcgtcgtc gatctccggc aaatcgacgc caccggctcg
     1081 ctgaccggcc gccatcaact gctcgatcca tgtgaggtct gcgtcgggag aacggtcgag
     1141 agcctcaaga aggatcgtcc gggcctcggc ctccatcgag cgaccgttgg tggcagcgcg
     1201 catgcgcaac tgctgcttgg cagccaaggg cagctgccga atggtgatgg ccgtcgtcat
     1261 gtagcaatga tatcactgca ccattctccg ccccggagtc cgtcaggcgt cgaggaacca
     1321 ggcctgcgcc attcccgcgg gccggcccgg ctcgatgtag tgctccctgc caaagaagcc
     1381 cgcaaagacc tgctccggca tcgagagcat ccccccgaca tcggtggtct gcgaggcatg
     1441 gcaagacagt gccgcgcggc gctgcggcag gtatgccgcg acatccacct gccagtggat
     1501 ctccgcctcg ggtgatccca tcgggttgcc gtcatccatc ggcccatcgg ggtcgaagcc
     1561 gttctcgccg tcgtccatcc ccgcggcgcg ggccgcggcc acgaagctgc gcatgatgtc
     1621 gcggttcatc gtggcctcga gcaggcgagg tcggagggcg gcgagttcgg cagcccggtg
     1681 aacgaccttg tggacctgga cgtggtcggg gtgcccgtag ccaccgtgcc agtcatagcc
     1741 gatcaacacg tcggccgact cgtcgtcgag gatggcggcc agccgcccgg cagcctcgtc
     1801 aagcggggcc tggacgaagg cgtgctcgtg gtcgttctgg tcccagccgg tcatccccga
     1861 gtcgacatac cccagccatt cgacccggtg cacacccagc gcggccgccg acccggccaa
     1921 ctcggcccga cgccgctgcg cgacggtctc ccccggggcg aggtctgcgg cggcctcccc
     1981 gtgctcgccg cctgtcgaca cgaccaacac cactcggtgg ccctcctcga cggcgcggca
     2041 catcgccccg gcggtctggg acgcctcatc gtcgggatgg gcgtgaacga aaacaagagt
     2101 cgacataagc gctc
//
LOCUS       DAOMZY010000445         2080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_56713_length_2080_cov_4.958025, whole genome shotgun sequence.
ACCESSION   DAOMZY010000445 DAOMZY010000000
VERSION     DAOMZY010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56713_length_2080_cov_4.958025"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..632
                     /locus_tag="PLK64_16745"
     CDS             177..632
                     /locus_tag="PLK64_16745"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HPV81443.1"
                     /translation="MPPKPPNRFAAFANSPKVAAGFLAAVALACTIGTVVAYQSAVAL
                     RDHGQRAIGSVLEVHEERRDDYVVLRFQDGTGREVTAEVGNYFWDPQPQVGDRTELVY
                     DPEDPSGNVADVRTGPDFFSVWALALGAVLAGVLVWPTWTGRLDWNKFR"
     gene            complement(729..1262)
                     /locus_tag="PLK64_16750"
     CDS             complement(729..1262)
                     /locus_tag="PLK64_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 domain-containing protein"
                     /protein_id="HPV81444.1"
                     /translation="MTVRLLDEQQASELRAAPLSYTPRGGPEVGLPTGYNHLNRSVTL
                     SRRDFDGAAGDLFAWRMHSEAGLQVQASDVPLRQGTVVLMRWGRGSLSVKIPCRVLDV
                     VDETRYRGFTYGTLPGHPEAGEERFLLEQLEDGRILFTITAYSRPASTLAKLGGPITR
                     AAQRFMTQRYLQALDRV"
     gene            1346..1993
                     /locus_tag="PLK64_16755"
     CDS             1346..1993
                     /locus_tag="PLK64_16755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81445.1"
                     /translation="MTALLGGAANPLGAVVQFIHAPIGNVADAVASRFTAGPANASLM
                     DALSLLLPFESPWSRILLAPCGDWTALVNNGLYGGDSTAPGSAISRALNVECVVASAV
                     PRYGPGHEQTQMEVFGPAGEPPLMYIRSLSATATDGRWEWYTSGTPFEFEATERYTRW
                     RKRDRFDRELLLDYLEHLGIPARMDTAYGPAILLQERAQYERRKMTLEEARADFR"
BASE COUNT          355 a          697 c          668 g          360 t
ORIGIN      
        1 gaggacgggc cgctcatcgg cgaagacatg cgtgcagacg tagcacttcg ggtcgcccat
       61 ccgccgacag tactgagacc cccttcggac tgcacgatcc gcggcagatg aggacgttgc
      121 catgacaccc cttaccgcgt cgtgtccttg aacttccctt gctagggtgc cgccacgtgc
      181 cacccaagcc gcccaacagg tttgccgcct ttgctaacag tccgaaggtc gcggcggggt
      241 ttctcgcggc ggtcgctctc gcttgcacca tcggaacggt cgtcgcctat cagtccgccg
      301 tcgcactcag ggaccacggc caacgagcca tcggttcggt cctcgaggtg cacgaggagc
      361 gccgagacga ctacgtagtt cttcgctttc aggacggtac tggccgcgag gtcacggccg
      421 aggttgggaa ctacttctgg gacccccagc cgcaagttgg cgaccgcacg gaactcgtgt
      481 acgaccccga agacccctca ggcaatgtcg ccgacgtcag gacgggcccg gacttctttt
      541 cggtctgggc actggctctt ggcgcggttc ttgccggtgt gctcgtctgg ccgacgtgga
      601 ctggccgatt ggattggaac aagttccgct aaccgcgtcg ccactcgcct cgtacggccc
      661 catgctggcc ggcccaccgc accccctcgg ctcctctggg accggatcgc ggcagttccg
      721 gacgcctgct acacgcggtc caaggcctgg aggtaacgct gcgtcatgaa gcgctgagcg
      781 gctcgggtga tcgggccacc gagcttggcc agcgttgagg ccggccgtga gtacgcagtg
      841 atggtgaaca ggatgcggcc gtcctcgagc tgttcgagga ggaaccgttc ttcgccggcc
      901 tccgggtgac caggcagggt gccgtacgtg aagccccggt accgcgtctc gtcgacgaca
      961 tcgaggacgc gacatgggat cttcaccgaa agggatcccc gtccccatcg cataagcacc
     1021 acggtgccct gccgcagagg gacgtccgag gcttgcactt gtagcccagc ctcggagtgc
     1081 atccgccatg cgaagagatc gcctgcggcg ccgtcgaagt cgcggcgact cagcgtgact
     1141 gagcggttca ggtggttgta gccggtcggc aagccgactt ctggtcctcc tcgcggtgtg
     1201 taggacaaag gcgccgcacg cagctcggat gcctgctgct cgtcgaggag gcgcactgtc
     1261 atagccacat catcaccgac cgcggcttac gcaccacccg cggcagaaga gggcactgca
     1321 aaagtgctgt cgctagtgtc caaacatgac tgccttgctc ggaggagccg ccaacccgct
     1381 cggagccgtc gtgcagttca tccacgcccc gatcggcaac gttgccgacg cggtcgcatc
     1441 gcgattcacc gcgggccccg cgaatgccag cctgatggac gcgctcagcc tgctgctgcc
     1501 gttcgagagc ccatggtcgc gaatcctgct cgccccgtgt ggcgactgga cggcgcttgt
     1561 caacaacggg ctgtacggcg gcgactcaac cgctcctgga tctgcgatca gccgcgcgct
     1621 gaacgttgag tgcgtcgtgg ccagcgctgt cccacgttat gggcccggtc acgagcagac
     1681 acagatggag gtcttcggcc ctgcgggtga gcctccgttg atgtacatcc gctcactctc
     1741 ggccacagcc accgacgggc gatgggagtg gtacacgtcg ggaacgccgt tcgagttcga
     1801 ggccaccgag cgttacaccc gctggcgcaa gcgcgaccgc ttcgatcgcg agttgctctt
     1861 ggactacctc gagcacctag gcatcccggc acgcatggac accgcgtacg ggccggcaat
     1921 cctgctccag gagcgcgcac agtacgagcg gcgcaagatg acgctggagg aggcccgcgc
     1981 cgacttcagg tagtgcccgc tcatcggcta atcaccaagg tcacaatccg cggcatgaca
     2041 ggacgagttc ggccaaggtg gcggcgcgac tccggggtct
//
LOCUS       DAOMZY010000446         2076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_56881_length_2076_cov_4.746165, whole genome shotgun sequence.
ACCESSION   DAOMZY010000446 DAOMZY010000000
VERSION     DAOMZY010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56881_length_2076_cov_4.746165"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..593
                     /locus_tag="PLK64_16760"
     CDS             12..593
                     /locus_tag="PLK64_16760"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HPV81446.1"
                     /translation="MVHWADGGASDLTNLALLCGRHHSIAHRDHLIGYVNAPCGAVDG
                     GAIDGTAVTWNVIPGSYDRRDDGPSAHGDQSSGDPPPGDPPPANQPPRVHPHGDPPVP
                     QCRQSPGHHEWDNPGPDAEPWADLWPDANPAAETRPADDAAGRCHGPDAEPWADLWAD
                     SGHRGDSEPTDHSDRYHPNPEADPWYGSGGPAA"
     gene            700..1986
                     /locus_tag="PLK64_16765"
     CDS             700..1986
                     /locus_tag="PLK64_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPV81447.1"
                     /translation="MKRAFVAPGFPRLYAALSTSALGDSILLLVLSMWVKTITGSNAA
                     AGLTFLFMLLPALVAPALGIWVDRVRAKPLLVWGNVLSALAVLPLVFVRSADQLWIIW
                     TVAFLYGVSFIVLPAGVNGLLKELIADDVLVDANAALQTTKEGFRLFGPLAGAALFAW
                     TGGWLVAVIDAASFLIAAVVIASVKVQEAAPAPEGGHIWVQLTAGVRHLVGDRALRHT
                     LVGFGIMLLVLGFTESSIYAVLDAFGKPAAFAAVLVTFQGVGAVIGGLTSNLVVRRLG
                     EVGTTALGMVVLAVPLGVIASATNVNVALAALVVVGVSIPWLFVAVTTLVQRRTPQAV
                     IGRASMALEVLMGTPQAISLAVGALLVVFVSFRTIFVIIALATLAGASYIAIMLRREI
                     AADLATSRDRRAEIGGLGPAVDAAPRADLPTPESPA"
BASE COUNT          288 a          784 c          650 g          354 t
ORIGIN      
        1 acgctcacca cctggtccat tgggccgacg gcggggcatc cgacctcacc aacctggcgt
       61 tgttgtgcgg gcgacaccac tccatcgccc accgagacca cctcatcggt tatgtcaacg
      121 ccccctgcgg tgctgtcgat ggcggtgcta tcgatggaac tgctgtcacc tggaacgtca
      181 tccccggaag ctacgaccgc cgcgacgacg gaccctcagc gcacggtgac cagtcatcgg
      241 gcgacccacc acccggcgac ccaccaccag cgaaccaacc accacgggtc catccccacg
      301 gtgacccgcc cgtaccccag tgccgtcaat caccaggtca ccacgagtgg gacaaccctg
      361 gccccgacgc ggagccctgg gcagatctct ggccagacgc gaacccagcg gccgagaccc
      421 ggccggcgga cgatgctgcc gggcgctgtc acgggcccga cgccgagccc tgggcagacc
      481 tgtgggcgga ctcgggacac cgcggcgaca gcgagcccac ggatcactcg gaccgatacc
      541 accccaaccc cgaagcagac ccgtggtacg gctcaggcgg cccggccgcg taaccgggcc
      601 gcctccgacc ttgccgactg catgaacacg atataacgtg tttctggcag tcgcgacata
      661 tcgtgcctgc atcggcccag ccccggacga ggagtccccg tgaaacgagc cttcgttgcg
      721 cccggcttcc ctcgtctgta tgccgcgctg agcacctccg cgctcggcga ctcgatcctg
      781 ctgctcgtcc tgtcgatgtg ggtcaagacg atcaccggct cgaacgccgc ggctggcctg
      841 accttcctct tcatgctcct cccggcgctc gtcgccccgg ctctgggcat ctgggtcgac
      901 cgggttcgcg ccaagccgct gctcgtctgg ggcaatgtcc tctcggccct cgcagtcctg
      961 cccctcgtct tcgtccgctc ggccgaccaa ctctggatca tctggaccgt cgcgttcctc
     1021 tacggcgtgt ccttcatcgt cctgcccgcc ggagtcaacg gcctcctcaa ggaactcatc
     1081 gccgacgacg tcctcgtcga tgccaacgct gccctgcaga cgaccaagga gggctttcgg
     1141 ctcttcggcc cgctcgcggg ggcggccctg ttcgcgtgga cggggggttg gctcgtggca
     1201 gtgatcgacg ccgcgtcatt cctcatcgct gcagtcgtca tcgcctccgt caaggtgcag
     1261 gaggcggcgc ccgcacccga gggcgggcac atctgggtcc aactcaccgc cggagtccgt
     1321 cacctcgtcg gagaccgggc cctgcggcat accctcgtgg gctttgggat catgctgctc
     1381 gtcctggggt tcaccgagtc gtccatctat gcggtgctgg acgccttcgg gaagccggcg
     1441 gcgttcgccg cggtgctggt caccttccag ggggtgggag ctgtcatcgg tgggctcacc
     1501 tccaacctcg tggtccgtcg gctcggtgag gtcgggacca ccgccctggg catggtggtg
     1561 cttgcggtcc ccctcggcgt catcgcgagc gcgaccaacg tgaacgtcgc gctggccgcg
     1621 ctcgtcgtcg tcggtgtctc gatcccctgg ctgttcgtcg ccgtgaccac cctcgtccaa
     1681 cgccgcaccc ctcaggccgt gatcggccgg gcgtcgatgg cgctcgaggt gctgatgggc
     1741 accccgcagg ccatctcgct ggccgtcgga gcgctgctcg tcgtcttcgt ctcgttccgc
     1801 acgattttcg tcatcatcgc gctggccacg ctggcgggcg ccagctacat cgcgatcatg
     1861 ctgcgccgcg agatcgcggc cgacctcgcc acctcgcgtg atcggcgcgc cgagatcggt
     1921 gggctcggcc cggccgtgga tgcggcgcca agagctgacc tcccgacgcc cgagtcaccc
     1981 gcgtagtcgt gccgtcagcg cggccgccac ccggcgcacc tgctcgacca accggtcccg
     2041 gccggcggtg tcgacgacgt ccgacgggta ggtcac
//
LOCUS       DAOMZY010000447         2045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_58287_length_2045_cov_3.637186, whole genome shotgun sequence.
ACCESSION   DAOMZY010000447 DAOMZY010000000
VERSION     DAOMZY010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58287_length_2045_cov_3.637186"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(413..>2045)
                     /locus_tag="PLK64_16770"
     CDS             complement(413..>2045)
                     /locus_tag="PLK64_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81448.1"
                     /translation="KTPPRSGRRLFVFPDLVKGGDAAGLTKRIIGLTNKALVLSQETV
                     ANAEAAAKALAGEGVPVMGRDDLEQQGLDSFAAASAGVLGLANRYDGLDLPGSACRIV
                     VLGGKPDAVGLQERFLSERAEANAALAERVRTRIVQGAGRCTRGPNDFAVVVVLGSDI
                     MKYFSRPDNLKALEPELQAEVEFGWQNSKGADPDEVVENVVTFLEHDSDWREQGEPMV
                     AEFRREAVKVEQPAAEALGKAAALEVEAWQLAFGGDWTGASEKLQEAVPHVGPEATRG
                     YRGLLLYIAGVWLHLGAEDEAQRARARQLVRGAALAANRGTWLTEMRELPGAEKIPLA
                     PRDVVAVDAIVARLRGQLKPKRTNAALMEMQAALAQDEAARFERGLTTLGTFLGAEAS
                     KPKGQGRCDSAWVWGTATWMTIEAKSEEHDDGLLPLKDVRQANTQLDQLTADRGMDHA
                     PPGSPAIIVSDRLSVDPQHAPAANPNVYLASTDVVKEIASDVTVLWGDLLTTAAGIAS
                     EQAQKQHVRSVMTENGCLPSQVIDRLTQNRIRPGE"
BASE COUNT          328 a          710 c          634 g          373 t
ORIGIN      
        1 cccttgaccg accgcttgcc gtcctcgagc ttcggcaagg gtccgaaggc gcccttccgc
       61 ccaccgccac acagcgtctc agcgcccaat tgggggaacg tgcctgaaag ccggataggg
      121 ccatttggac tgctgagggg tcaggcggga tcctgacccc tcagcagtcc cgtcgggtgg
      181 gcgcattagg gatgcggctc cggtgtcatc tcgcagcagt aggagcttgc ccaactggtc
      241 cggcacgtcc gacggcccct cagctcgtca cctggagcgc tcggcacggc tcccgaggac
      301 ctccaccttc ggcccggggc cattctgacg accgatggat ggagcagctt tgatacgaga
      361 aagggccgaa ctgtagtcgg gtgccgaggt ggccactgga tgtcgcatcg gatcactcac
      421 cgggcctgat ccggttctga gtgagccggt caatcacctg gctgggcaga cacccgttct
      481 ccgtcataac ggagcggaca tgctgctttt gtgcctgctc cgaagcgatc ccggcggcgg
      541 tcgtgagcag gtcaccccac agcacggtga cgtcgctggc gatctccttc accacgtccg
      601 ttgaggcaag gtagacgttg gggttcgccg ccggcgcgtg ctgcggatcc accgacagtc
      661 tgtccgacac gatgatcgcc gggctacccg gtggcgcgtg atccatcccg cggtctgccg
      721 tcagctggtc gagctgggtg ttggcctgac gcacgtcctt gagggggagc aggccgtcgt
      781 cgtgttcttc tgacttcgct tcgatagtca tccatgtggc ggtgccccat acccaggcgg
      841 agtcgcaacg gccttgaccc ttgggcttgg acgcctcggc cccgaggaat gtcccaaggg
      901 tcgtcaggcc gcgctcgaat ctagccgcct cgtcttgggc gagcgccgcc tgcatctcca
      961 tgagggcagc gttagtgcgc ttcggcttga gctggccgcg caggcgggcg acgatcgcgt
     1021 ctactgccac cacgtccctt ggggcgagtg ggatcttctc ggcaccgggc agctctcgca
     1081 tttccgtgag ccaggtgccg cggttggcgg ccagtgcggc cccgcgtacg agttgccgtg
     1141 cacgtgcccg ctgggcctca tcctcagccc cgagatgcag ccacacccca gcgatgtaaa
     1201 gcagcagccc cctatagcca cgggtcgcct ccgggccgac gtgtggaacc gcctcctgga
     1261 gcttctcact ggcgccggtc cagtccccgc cgaacgccag ctgccatgcc tcaacttcga
     1321 gtgcagcggc cttaccgaga gcctcagccg ccggctgctc gacctttacg gcctcgcgac
     1381 ggaactcggc caccatcggc tcgccctgct cccgccagtc tgagtcatgc tccaggaatg
     1441 tcacgacatt ctcgacgacc tcgtcgggat cggcgccctt gctgttctgc caaccgaact
     1501 ccacctcagc ctgcagctcc ggctcgaggg ccttgaggtt gtcgggccga gagaagtact
     1561 tcatgatgtc cgacccgagc acgaccacta cggcgaagtc attcggaccc cgagtacatc
     1621 ggccagcacc ctgcactatg cgagtccgaa cacgctctgc gagagcagcg ttagcctcag
     1681 ctcgctcgct gagaaatcgc tcttgcagcc cgacagcatc gggtttccca ccaaggacga
     1741 cgatccgaca cgcgctcccc ggcaggtcca gtccgtcgta ccggttagcc aatccgagga
     1801 cgcccgccga ggcagcagcg aaggagtcga gaccttgctg ttccaagtcg tcccgtccca
     1861 taaccggcac gccttcgccc gccaaggcct tagctgccgc ctcggcgttg gcgacggtct
     1921 cctggctgag cacaagtgcc ttgttggtga ggccgatgat gcgtttcgtc agcccggccg
     1981 catccccacc cttcaccaga tccgggaaca cgaacagtcg tcgaccggag cgcggagggg
     2041 tcttg
//
LOCUS       DAOMZY010000448         1985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_61183_length_1985_cov_5.265803, whole genome shotgun sequence.
ACCESSION   DAOMZY010000448 DAOMZY010000000
VERSION     DAOMZY010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61183_length_1985_cov_5.265803"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1132)
                     /locus_tag="PLK64_16775"
     CDS             complement(125..1132)
                     /locus_tag="PLK64_16775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HPV81449.1"
                     /translation="MAPISTAGHLPAPTAVTEHEHTLVVAQREQASDDVLALTLAALD
                     GSPLTPWAAGAHIDLILPTPTGELVRQYSLCSSPGDLTSYRLGILHEPAGRGGSAYIH
                     DGLRVGDTVRVAGPRNHFELVEAASYVLIAGGIGVTPLLPMVAALAASGADWHLYYVG
                     RSRSTMAFCDEVEQYADHVTLVPRDVAPRPDLAALLATPVPGRVVYSCGPESLIGWLE
                     QTMAANGWAPGALHTERFEAQEIDTSGDLPFEVELSRTGKSLLVPADSSIFQVAREAG
                     VSVLGSCLEGICGTCETEILEADGELIHRDSVLTLDDQASGEVMMICVSRCTGKVVLD
                     M"
     gene            1248..1598
                     /locus_tag="PLK64_16780"
     CDS             1248..1598
                     /locus_tag="PLK64_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPV81450.1"
                     /translation="MDSIPDGPQEGMPLVEVGSLADDAVILDVRDVGDWRAGHAPGAV
                     NIPLAQLISRLTELPVPPTGPLAVSCGGGTKQTRAVAYLRAHGIDAVVLKGGMRSWKA
                     AGRPVESADGDERG"
     gene            1646..>1985
                     /locus_tag="PLK64_16785"
     CDS             1646..>1985
                     /locus_tag="PLK64_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HPV81451.1"
                     /translation="MKILSIQSHVAFGHAGNSAAVFPLQRLGHDVYPVLTVTFSNHTG
                     YGATRGPLIAPADVAEVILGVEERGAFAELDAVLSGYMGAETMGAVVLDAVDRVRAAR
                     SAAGRPAAVYC"
BASE COUNT          291 a          662 c          739 g          293 t
ORIGIN      
        1 cctgcacgac acgcacctgc acgacacgca cctgcacgac acggagccgg gcccctcacc
       61 gatggtgggg gacccggttt ctgttggcgt atgccgtggt cgcgccgatc cgcgccgcgc
      121 aggcttacat gtccaggacg accttgcccg tgcaccgcga cacgcagatc atcatgactt
      181 cgccggaggc ttggtcatcg agggtgagga ccgagtcccg gtggatcaac tcaccgtcgg
      241 cctcgaggat ctcggtctcg caggtgccgc agatgccctc caggcatgac ccgaggacgc
      301 tcaccccggc ctcgcgggcg acctggaaga tggaggagtc ggcagggacg agcaggctct
      361 tgcccgtgcg cgacaactcg acctcgaagg gcaggtcgcc gctggtgtcg atctcctggg
      421 cctcgaaccg ctcggtgtgc agggcccccg gcgcccatcc gttggcggcc atcgtctgct
      481 ccagccagcc gatgagcgac tcggggccgc aggagtagac gacccgcccc gggaccgggg
      541 tggccagcag cgcggcgagg tcgggccgcg gcgcgacgtc ccgcggcacg agggtgacgt
      601 ggtcggcata ctgctccacc tcgtcgcaga aggccatggt ggagcgtgag cgaccgacgt
      661 agtagaggtg ccagtcggcc ccgctcgcgg cgagggcggc caccatcggc aacagggggg
      721 tgacgccgat gccgcccgcg atcaggacgt aggaggccgc ctcgaccagc tcgaagtggt
      781 tgcgggggcc agcgacgcgg acggtgtcgc ccacgcgcag gccgtcgtgg atgtatgccg
      841 atccgccgcg gcccgcgggc tcatgcagga tccccaggcg gtaggacgtc aggtcgccgg
      901 ggctggagca gagcgaatac tgccggacga gctcccccgt cggcgtcggc aggatgaggt
      961 cgatgtgggc gcccgcggcc cacggggtga gcggggagcc gtcgagggcg gccagggtga
     1021 gcgccaggac atcgtcgctg gcctgctcgc gctgggccac gacgagcgtg tgctcgtgct
     1081 cggtcacggc cgtgggagcc ggcaggtgac ccgcggtcga gatcggcgcc atcggcagcc
     1141 cctttgcgtg tgatggatca cgtcgtttgc acccaaacga taatggtcgt gcgtcaggtg
     1201 tcaaccacct cgccccgcgc tgaaggctgg gatgggaaga tcgggggatg gactcgatcc
     1261 cggacggacc ccaggagggt atgccgctcg tcgaggtcgg ctcgctcgcc gacgacgcgg
     1321 tcatcctcga cgtgcgcgac gtcggcgact ggcgggccgg gcacgctcct ggggcggtga
     1381 acatccctct ggcgcagttg atctcgcggc tcaccgagct gcccgtcccg ccgaccgggc
     1441 cgctggccgt gtcgtgcggg ggtggcacca agcagacccg ggcggtggcg tatctgcggg
     1501 cccatggcat cgacgcggtc gtcctcaagg gcgggatgcg cagttggaag gccgcggggc
     1561 gacccgtcga gtcggccgat ggcgacgagc gcggttaacc cacctggcgc ggcgtgaaaa
     1621 acacactggc agaacggcat acgagatgaa gatcctgtcc atccagtcgc acgtggcctt
     1681 cggtcatgcg ggcaactccg cggctgtctt cccgttgcag cggctgggcc acgacgtcta
     1741 tccggtgctg accgtgacgt tctccaacca caccggttat ggggcaacgc gcggcccgct
     1801 gatcgcgccc gcggacgtcg ccgaggtcat cctgggcgtc gaggagcgcg gggcgttcgc
     1861 cgagttggat gccgtgctgt ccggctacat gggggccgag accatgggcg cggtcgttct
     1921 cgatgccgtc gaccgggtgc gggctgcgcg ctcggccgcc gggcgccccg ccgcggtcta
     1981 ctgct
//
LOCUS       DAOMZY010000449         1956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_62673_length_1956_cov_4.844292, whole genome shotgun sequence.
ACCESSION   DAOMZY010000449 DAOMZY010000000
VERSION     DAOMZY010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62673_length_1956_cov_4.844292"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /locus_tag="PLK64_16790"
     CDS             <1..1253
                     /locus_tag="PLK64_16790"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HPV81452.1"
                     /translation="ELRDLFGIEPVGHPLPRGLVRHGHWPTTWHPMLRDAGPMPDFEP
                     DVGSYPFVHVQGDGVYEIPVGPIHAGIIEPGHFRFSVVGETILRMKARLWFVHRGMER
                     LFEGRLPSEAIELAERISGDTAVGHTVAFVQAVEEALGIPIDPEVAARRALLLELERL
                     YNHVADLGMIPNDVGFGIAHIHTQRLREKLLRHNAAVTGHRLLRGGITVGDTRFVREP
                     DLALVAGVAAEMAEIVAFTLNHSVVTDRFTGTAVLRADLGGSLGVLGYVARASGIGVD
                     ARRDHPSVDLGPDFSVVTQEGGDVLSRYAVRAGEVAVSAGILPHLAELASGGTGGERA
                     AYPVTDGTTGGPAAGLGVVEAWRGTLCHRVELAPDGRLTRVKIVDPSFFNWPGLPVAL
                     ADTIVPDFPLANKSFNQSYAGNDL"
     gene            1305..>1956
                     /locus_tag="PLK64_16795"
     CDS             1305..>1956
                     /locus_tag="PLK64_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPV81453.1"
                     /translation="MDDSSGPLTRAVASAALPTWRYLLGRMHLSVATGDFATALALVD
                     RVGAIAEEQGHHPEIDLRWGRVHIATGSHDVGGVTERDIRLGKAIDAVVQEMGLTSQP
                     GRLTEVEIAIDAMDRDAIRPFWAAVLDRPVNGGDELVDPDRVGPAVWFQQMDSPRPQR
                     NRIHLDVTVSHDEAPGRLARALAAGGRLVSDDRAPAFWVLADAEGNEACICTWQSRD"
BASE COUNT          283 a          678 c          685 g          310 t
ORIGIN      
        1 gcgagctgcg cgacctgttc gggatcgagc cggtcgggca ccctctgccg cgagggctgg
       61 tccggcacgg acactggccg acgacctggc acccgatgct gcgcgacgcc gggccgatgc
      121 ccgatttcga gcccgacgtc gggtcctatc cgttcgtcca tgtccagggt gacggcgtct
      181 acgagatccc ggtgggcccg atccacgcgg gcatcatcga gccggggcac ttccggttct
      241 cggtggtcgg cgagaccatc ctgcggatga aggcccggct gtggttcgtc caccgtggca
      301 tggagcggct gttcgagggt cgattgcctt ccgaagcaat cgaattggcc gagcggatca
      361 gcggcgacac ggcggtcggg cacacggtcg cgttcgtcca ggcagtcgag gaggccctcg
      421 gcatcccgat cgaccccgag gttgctgcga ggagggcgct cctgctcgag ttggagcggc
      481 tctataacca cgtcgccgac ctcgggatga tccccaacga cgtcggcttc gggatcgccc
      541 acatccatac ccagcggctg cgggagaaac tgttgcggca caacgctgct gtgaccgggc
      601 accggctgct gcgcggcggg atcaccgtcg gcgacacacg gttcgtcagg gagccggacc
      661 tcgcgctggt cgctggggtg gctgccgaga tggccgagat cgtcgcgttc acgcttaacc
      721 actcggtcgt caccgatcgg ttcaccggca ccgcggtctt gcgggccgac ctgggtggca
      781 gcctcggcgt gctcggctac gtcgcgcggg ccagcggcat cggtgtcgac gcgcggcgcg
      841 accacccgag cgtcgacctc ggacccgact tctcggtcgt cacccaggag ggtggcgacg
      901 tcctctccag gtatgccgtc cgcgccggcg aggtcgccgt gtccgccggg atcctccccc
      961 accttgccga gttggcttcc ggcggaacgg gaggtgagcg ggcggcatac cccgtgacgg
     1021 acgggaccac gggcggaccg gcggccgggc tcggtgttgt tgaggcctgg cgcggcacgc
     1081 tgtgtcaccg ggtggagctg gcgccggacg gacggctcac ccgggtgaag atcgtcgacc
     1141 cgagcttctt caactggccg gggctgccgg tggcgctggc cgacacgatc gtcccggact
     1201 tccccttggc caacaagagt ttcaaccagt cctacgcggg gaacgacctc taacctcggc
     1261 cccagaaagt ccccacgagt cgctgatgcg cttagggtgc ccagatggac gactcatccg
     1321 gccccctgac ccgcgccgtt gcgtcagcgg ctctccccac ctggcgctat ctgctgggcc
     1381 ggatgcacct gagcgtcgcc acgggcgact tcgccacggc gttggcgctc gtcgaccggg
     1441 tcggcgccat cgccgaggag cagggacacc accccgagat cgacctgcgg tggggtcgcg
     1501 tgcacatcgc caccggaagc cacgacgtag gcggcgtcac ggagcgggac attcgcctcg
     1561 ggaaagccat cgatgccgtc gtgcaggaga tgggcctcac cagccagccg ggccgcctga
     1621 ctgaggtcga gatcgccatc gacgctatgg atcgcgacgc gatccgaccg ttctgggccg
     1681 cggtgctcga tcggcccgtc aacggtggcg acgagcttgt cgaccctgac cgggtcgggc
     1741 cggccgtgtg gttccagcag atggattcgc ctcgcccgca acgcaatcgg atccacctgg
     1801 atgtgaccgt cagccatgac gaggcccccg gccggctggc ccgcgccctt gccgccggag
     1861 gtcgactcgt cagcgacgac cgcgctccgg cgttttgggt cctcgccgac gccgagggca
     1921 acgaggcctg catctgcacc tggcagagcc gcgact
//
LOCUS       DAOMZY010000450         1953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_62843_length_1953_cov_6.575869, whole genome shotgun sequence.
ACCESSION   DAOMZY010000450 DAOMZY010000000
VERSION     DAOMZY010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62843_length_1953_cov_6.575869"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..>1953
                     /locus_tag="PLK64_16800"
     CDS             113..>1953
                     /locus_tag="PLK64_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPV81454.1"
                     /translation="MSTAAAASTAGIPTPGPSSDPAPVSPSALPERARVVVIGGGVIG
                     TSIAYHLAHLGWTDVVVLERDRLTSGTTWHAAGLMTCFGSTSHTSTAIRLYGRDLYAR
                     LEAETGLATGFKPVGLIEAAADEGRLHEYRRIAAFQRTQGLEVHEITPREIADLFPLA
                     KTDDLAAGFYVPGDGRVNPVDLTMSLARGAKQLGVRIIEGVSAESVVTHNNQVTAVRV
                     TQGDGTADIECEYAVNAAGMWARELGQRNGVVIPNQAAEHYYLITEPIDGVSPDAPVF
                     EDPAAYGYYREEGGGLMVGLFEPRAAAWKVGGIPQDFSFGTLEPDWERMEPFLRTAME
                     RVPVTLDAGIRTFFCGPESFTPDLLPAVGEAPGIRGYFVCAGLNSIGILSSGGMGRLV
                     AQWITSGHPDADVTAFNVDRFQAWQLAPRLRAARTSEVLGTVYAAHAPGTQLRSGRGI
                     KRSPLHDKLIAQGAYLKDVSGWESPDWYAAPGTTPTAQPSWQHEPWFEQWRVEHEAVR
                     TGVGLIDMSFMAKFIVEGPDAATVLDRISAGPVAGQPGRIVYTQWLNDTGGIEADLTV
                     TRLADDRFLVVASDTAHGHAAAWLARHTEGADVRVSDVTATYAQVNI"
BASE COUNT          310 a          747 c          603 g          293 t
ORIGIN      
        1 ggggtcgggc ggcggctggg agcaggaagt catcggcgag tgtgaggaac tgggggaagg
       61 gtgggttggg cggagtgctt cgggccgtga cggccagcga taggttcgtt cggtgagcac
      121 cgccgccgca gcctcgaccg caggcatccc tacccccggc ccatcgtccg acccagcccc
      181 ggtctcgcct tcggcgctgc ccgaacgagc cagagtcgtc gtcatcgggg gcggggtcat
      241 cggcacctcg atcgcctatc acctggccca cctcggctgg accgacgtcg tcgtcctcga
      301 acgcgaccga ctcacctccg gcaccacctg gcacgcggcc ggcctgatga cctgcttcgg
      361 ctcgacgtcg cacacctcga ccgcgatccg cctctacggc cgcgatctct acgcccggct
      421 cgaagccgag accggcctgg ccaccggttt caagccggtt gggctcatcg aggcagccgc
      481 cgacgaggga cgcctgcacg aataccgccg gatcgccgcc ttccaacgca cccaggggct
      541 tgaggtccac gagatcaccc ctcgcgagat tgccgacctc ttccctctcg ccaagaccga
      601 cgacctcgcc gcgggcttct atgtccccgg cgacgggcgg gtcaacccgg tcgacctcac
      661 gatgtccctc gcccgcggcg ccaaacaact cggcgtccgc atcattgaag gcgtctcggc
      721 cgaatccgtt gtcacgcaca acaatcaggt caccgcagtc cgggtcaccc agggtgacgg
      781 caccgccgac atcgagtgcg agtatgccgt caacgccgcc ggtatgtggg cccgcgagct
      841 cggccagcgc aacggggtcg tcatccccaa ccaggccgcc gagcactact acctcatcac
      901 cgaacccatc gacggggtca gccccgacgc cccagtcttc gaggacccag cggcctacgg
      961 ctactaccgc gaggagggcg gcggcctcat ggtcggcctc ttcgagcccc gcgcggcggc
     1021 ctggaaggtg ggcggcatac cccaggactt ctccttcggc accctcgaac ccgactggga
     1081 gcgcatggag ccgttcctgc gcaccgcgat ggagcgggtc cccgtcaccc tcgacgccgg
     1141 catccgcacc ttcttctgcg ggcccgagtc gttcaccccc gacctgctcc ccgcggtcgg
     1201 cgaggcgccc ggaatccgcg gctacttcgt ctgtgcgggt ctcaactcga tcggcatcct
     1261 ctccagcggc ggcatgggcc gccttgtcgc gcaatggatc acgtccggtc accccgacgc
     1321 cgacgtcacc gccttcaacg tcgatcggtt ccaggcctgg cagctcgctc cgcggctgcg
     1381 ggccgcgcgc accagcgagg tcctcggcac ggtctacgcc gcccacgcac ccggcaccca
     1441 actgcgctcc ggccgcggca tcaagcgaag cccgttgcac gacaagctga ttgcccaggg
     1501 cgcctacctc aaggatgtct ccggttggga atcgcccgat tggtatgccg cccccggcac
     1561 caccccgacc gcccaaccgt cgtggcaaca cgaaccctgg ttcgagcagt ggcgggtcga
     1621 gcatgaggcc gtccgcaccg gcgtcggcct catcgacatg tccttcatgg ccaagttcat
     1681 cgtcgaaggc cccgatgctg caacggttct cgaccgaatc agcgcgggcc ccgttgccgg
     1741 acaaccgggt cggatcgtct acacccaatg gctcaatgac accgggggaa ttgaggccga
     1801 cctcaccgtc acccggctcg ccgacgaccg cttcctcgtc gtcgcctcgg acaccgccca
     1861 cgggcacgct gccgcctggc tcgcgcggca taccgaggga gcggacgtgc gcgtcagcga
     1921 cgtgacagcg acctatgcgc aggtcaacat cca
//
LOCUS       DAOMZY010000451         1929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_64126_length_1929_cov_5.457844, whole genome shotgun sequence.
ACCESSION   DAOMZY010000451 DAOMZY010000000
VERSION     DAOMZY010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64126_length_1929_cov_5.457844"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /gene="pdxS"
                     /locus_tag="PLK64_16805"
     CDS             <1..712
                     /gene="pdxS"
                     /locus_tag="PLK64_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005565691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HPV81455.1"
                     /translation="SRMSDPDMIDGIIAAVSIPVMAKARIGHFVEAQVLQSLGVDYID
                     ESEVLTPADYTNHIDKWAFNVPFVCGATNLGEALRRITEGAAMIRSKGEAGTGDVSNA
                     TTHMRQIRGEINRLRSMTQDELYVAAKELQAPYDLVKEVALAGKLPVVLFTAGGIATP
                     ADAAMMMQLGAEGVFVGSGIFKSGNPAQRAEAIVKATTFYNDADIIAKVSRGLGEAMV
                     GINVEELPQSHRLAERGW"
     gene            821..1372
                     /locus_tag="PLK64_16810"
     CDS             821..1372
                     /locus_tag="PLK64_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81456.1"
                     /translation="MGILAAAAFSVWLAWFGWSRFLPVAAELRQRERAAQIPPSAERS
                     TVNATFDVLAFNARLPVRRPRHLALADGWLTFTVPGHSRPTLQVAAEHLWMQIDEKDR
                     LLVYGPSIARITISRVADVDGALQSRPIDDSPLVATLAANGVRVPLGSRPRLPQGPKP
                     ERANLTALRRRGPSWPWAHRPSR"
     gene            1384..>1929
                     /gene="pdxT"
                     /locus_tag="PLK64_16815"
     CDS             1384..>1929
                     /gene="pdxT"
                     /locus_tag="PLK64_16815"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HPV81457.1"
                     /translation="MTTAATIGVLALQGDVREHVHALEAVGATAVPVKLESELHGIQG
                     IVLPGGESTVIDKLLRSFGLFEPLRDRLRGGLPAYGSCAGMILLADRVTDATADQQTL
                     GGIDMTVRRNAFGRQVDSFEQDVGYADWSAPVHAVFIRAPWVEQVGESVEVLARVDGG
                     RADGRIVAVRHGALLATSFHPE"
BASE COUNT          294 a          641 c          672 g          322 t
ORIGIN      
        1 gtcgcggatg agcgaccccg acatgatcga cgggatcatc gcggcggtgt cgatcccggt
       61 gatggccaag gcccggatcg gtcacttcgt cgaggcgcag gtgctgcaga gcctgggcgt
      121 cgactacatc gacgagtccg aggtgctgac cccggccgac tacaccaacc acatcgacaa
      181 gtgggcgttc aacgtcccgt tcgtgtgcgg ggcgaccaac ctcggcgagg cgctgcgccg
      241 gatcaccgag ggcgcggcga tgatccgctc caagggtgag gcgggcacgg gtgacgtgtc
      301 caacgcgacg acccacatgc gtcagatccg cggcgagatc aaccggctgc ggtcgatgac
      361 ccaggacgag ttgtatgtcg cggcgaagga gttgcaggcg ccgtatgact tggtcaagga
      421 agtggctctg gccggcaagc tgcccgtcgt gctgttcacc gccggtggca tcgccacccc
      481 ggctgacgcg gcgatgatga tgcagttggg tgccgagggt gtcttcgtcg gctcgggcat
      541 cttcaagtcg ggcaacccgg cgcagcgggc cgaggccatc gtcaaggcga cgacgttcta
      601 caacgacgct gacatcatcg ccaaggtgtc gcgcggtctg ggcgaggcca tggtcggcat
      661 caacgtcgaa gagctgccgc agtcccaccg cctcgccgag cgcggctggt agaccctcgg
      721 tgcggctcgc gaaccgaccc caacctgggc gggcgctgat gtgatggtca cggtgattgt
      781 tccgatggcg gatcactggg tctccaacac gctcacagtg gtgggtatcc tggccgccgc
      841 ggcgttcagc gtctggttgg cgtggttcgg ttggtcgcgc tttctcccgg tcgcggcgga
      901 gctgcgacag cgcgaacggg ccgcccagat cccgccgtcg gcagagcgtt ccacggtgaa
      961 cgcgactttc gacgtgctcg ccttcaacgc acgcctgcca gtgcggcggc ctcggcacct
     1021 cgccctggcc gacggctggc tcaccttcac cgttccaggc cactcgcggc ccaccctgca
     1081 ggtcgcggcc gaacacttat ggatgcagat cgacgagaag gatcgactgc tggtctacgg
     1141 accctcgatc gccaggatca ccatctcaag ggtggcggac gtagatggtg ctctccagtc
     1201 ccgaccgata gacgacagcc cgctggtggc caccctcgcg gcgaacggtg tccgcgttcc
     1261 cttgggcagc cggccacggc tccctcaagg acccaagccg gaacgagcca acctcaccgc
     1321 actccgtcgc cgtggcccct cgtggccctg ggcgcaccgc ccgtcgagat agcctggctg
     1381 gttgtgacta ccgcagcgac cattggcgtc ctcgcgttgc agggcgatgt ccgagagcac
     1441 gtccatgccc tggaggcggt tggcgcgacc gccgtgccgg tcaagctcga gagcgagctg
     1501 cacggcatac agggcatcgt gcttccgggc ggggagtcca cggtcatcga caaactgctg
     1561 cgctcctttg ggctgttcga gccgttgcgg gatcggctgc gcggcgggct acccgcctac
     1621 ggctcctgtg ccgggatgat cctgctcgcg gaccgggtga ccgacgcgac ggccgatcag
     1681 cagaccctcg gcgggatcga catgacggtg cggcgcaacg cattcggccg tcaggtcgac
     1741 tcgttcgagc aggacgtcgg gtatgccgat tggtcggctc ccgtgcacgc cgtcttcatc
     1801 cgcgcgccct gggtcgagca ggtcggggag tcggtggagg tgctggcccg ggtcgatggc
     1861 gggcgcgccg acgggcggat cgtcgccgtg cgccacggcg cgcttctggc gacctcgttc
     1921 caccctgag
//
LOCUS       DAOMZY010000452         1737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_76194_length_1737_cov_5.315101, whole genome shotgun sequence.
ACCESSION   DAOMZY010000452 DAOMZY010000000
VERSION     DAOMZY010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76194_length_1737_cov_5.315101"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..1434
                     /locus_tag="PLK64_16820"
     CDS             127..1434
                     /locus_tag="PLK64_16820"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HPV81458.1"
                     /translation="MPAVVLVGAQWGDEGKGKATDLLGRRVDYVVKFNGGNNAGHTVV
                     IEQADGSTEKYALHLLPSGILTPSCTPVIGNGVVVDLEVLFREIDGLNARGVDTSKLI
                     VSADAHIITPYNRVLDKVTERFLGARKIGTTGRGIGPTYADKMSRTGIRVQDLFDRHI
                     LEQKVEAALLFKNQVLAKIYNRRAISVAEVVEELESYAARLAPMVRDTSLVLQEALDR
                     GETVLLEAGQATLLDVDFGTYPFVTSSNATAGGACTGSGIPPTRVARVIAILKAYTTR
                     VGEGPFPTELFDANGEFLRKVGTEYGTTTGRPRRCGWMDVVIGRYATRINGVTDFVVT
                     KLDVLTGLEEVPICVAYDVDGVRHNEMPVNQSDFHHAQPIYETLPGWWEDITGCRTFE
                     ELPTNAQAYVLRVEELIGARVSAIGVGPGRDAIIVRHDLLGED"
     gene            complement(1513..>1737)
                     /locus_tag="PLK64_16825"
     CDS             complement(1513..>1737)
                     /locus_tag="PLK64_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HPV81459.1"
                     /translation="YAAALASLDGSAQGRLRVLDPLSTKGLEWDATLVVDPDEIVRES
                     PGGIRTLYVVLTRAAHRMTVLQTRRRGDDH"
BASE COUNT          301 a          608 c          568 g          260 t
ORIGIN      
        1 gggcgaacag ggcgccccga cgagagtgcg gtgcacggca tacggacgcg gtgacctcag
       61 caccacgcgc cccggtagcc tgggatgtcg ctccggcgac cacctcgcga acggaaggac
      121 ccgcgcatgc ccgcagtcgt cctcgtcggc gcccagtggg gcgacgaagg caaggggaaa
      181 gccactgacc tgctcggtcg ccgcgtcgac tacgtcgtga agttcaacgg cggcaacaac
      241 gccggtcaca cggtcgtcat cgagcaggcc gacggcagca ccgagaagta cgcgctgcac
      301 ctgctgccgt cgggcatcct cacgccgagt tgcacgccgg ttatcggcaa tggcgtcgtc
      361 gtcgacctcg aggtgctctt ccgcgagatc gacggcctca atgcgcgtgg agtcgacacc
      421 tccaagctca tcgtcagcgc cgacgcgcac atcatcacgc cctacaaccg ggtgctcgac
      481 aaggtgaccg agcgcttcct cggtgcccgc aagatcggca ccaccggccg gggcatcggc
      541 cccacctatg ccgacaagat gtcgcgcacc ggcatccggg tccaggacct gttcgaccgg
      601 cacatcctcg agcagaaggt cgaggcggcg ctgctgttca aaaaccaggt gctcgccaag
      661 atctacaacc gacgggccat ctcggtcgcg gaggtggtcg aggaactcga gtcgtatgcc
      721 gctcggctgg ctcccatggt ccgcgacacg tcgttggtgc tccaggaggc gctcgaccgg
      781 ggcgagacgg tgctgctgga ggccggacaa gccacgctgc tggacgtcga cttcgggacc
      841 tacccgttcg tcacctcgtc caacgcgacg gccggcggcg cctgcaccgg ctcgggcatc
      901 ccgccgaccc gagtggcccg cgtcatcgcg atcctcaagg cctacacgac ccgggtgggg
      961 gagggtccct tccccaccga gttgttcgac gccaacggtg aattcctgcg caaggtgggc
     1021 acggaatacg gcacgacgac cggccgaccc cgccgctgcg gctggatgga cgtggtcatc
     1081 ggccgctatg ccacccggat caacggggtg accgacttcg tcgtcaccaa gctcgacgtc
     1141 ctcaccggcc tggaggaggt gccgatctgc gtcgcctatg acgtcgacgg cgtccgccac
     1201 aacgagatgc cggtcaacca gagcgacttc caccacgctc agccgatcta cgagacgttg
     1261 cccggctggt gggaggacat caccggctgc cgcaccttcg aggagctgcc gaccaacgct
     1321 caggcttacg tgctgcgggt cgaggagctc atcggcgctc gggtgtccgc gattggcgtc
     1381 ggccccggcc gtgacgcgat catcgtgcgc cacgatctcc tcggcgagga ctgagggtcc
     1441 gaccctcgcg aggaggtatg ccgccgcccc accgcgctgc cctcgactga ggcgcttccg
     1501 atcgggcaga cctcagtgat cgtcaccgcg tctgcgggtt tgaagcaccg tcatccggtg
     1561 tgccgcacgg gtaagaacca cataaagggt gcgtatgccg ccgggagact cgcgcacgat
     1621 ctcgtcgggg tcgacgacga gggtcgcatc ccattccaaa cccttggtcg acagcgggtc
     1681 gagcacccgc agccggcctt gcgcggagcc gtccaacgac gccagcgctg cggcata
//
LOCUS       DAOMZY010000453         1724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_77185_length_1724_cov_6.249251, whole genome shotgun sequence.
ACCESSION   DAOMZY010000453 DAOMZY010000000
VERSION     DAOMZY010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77185_length_1724_cov_6.249251"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="PLK64_16830"
     CDS             complement(<1..579)
                     /locus_tag="PLK64_16830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPV81460.1"
                     /translation="MSPRLSHLSRLRSVAPEVFDPYAAADPAVVAASLADAVPEVYWT
                     DPAQRPMRPDPRPVLHGTSHRADLVVIGGGFTGLWAALQALEDDPSATVVVLEAGRLG
                     TGASGRNGGFVSASLTHGLAQGVALWPDELPTLERLGTDNIAGLEASLTGYGIDADFH
                     RPGELTLSLTPNQDGAITEGYELHRAHGLAGEV"
     gene            complement(671..>1724)
                     /locus_tag="PLK64_16835"
     CDS             complement(671..>1724)
                     /locus_tag="PLK64_16835"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HPV81461.1"
                     /translation="IRRLRAAGAVIVGKTAMPEFGQFPHTESLAHGRTHNPWDLSRSP
                     GGSSGGSAAAVAAGMVPVAMGADGGGSIRIPASCCGLVGLKPSRGRVSVAPLTEHWFA
                     LVCLGALTRTAADSALVLDVISGATPTDRWRLAPPGMPFAQAAATPPGRLRVGWTTTP
                     VLPGGKVDPQVVSAVAALADRLAGLGHDVQHISPRWPAPTTAFLPQFYAGMRVEAGQV
                     EHPELLEPRTKATVRLSGWATDRVVARALRAGERVTAALDTMFADWDVLLTPTLPVPP
                     PPLGQLDGQGTLRSQIVSTPLVGFTTIFNVSGHPAASVPAGLTREGWPVGAQLVAGMG
                     QEGRLLALAAQLEAAP"
BASE COUNT          259 a          596 c          650 g          219 t
ORIGIN      
        1 gacctccccg gccaggccgt gggcacggtg caactcatag ccctcggtga tcgccccgtc
       61 ctggttgggg gtgagcgaca gggtcagctc gccgggtcgg tggaagtctg cgtcgatccc
      121 gtatcccgtg aggctggctt ccagcccggc gatgttgtcg gtgccgagcc gctcgagggt
      181 gggcagttcg tcgggccaga gggcgacgcc ttgggccagg ccgtgcgtga gcgacgcgga
      241 gacgaagccg ccgttgcgac cggacgcccc tgtccccagg cgaccagctt ccagaacgac
      301 gacggtggcg ctggggtcgt cctccaaggc ctgcaaggcg gcccagaggc cggtgaagcc
      361 accgccgatg acgaccaggt cggcccgatg tgaggtgccg tgcaggacgg gtcgggggtc
      421 cgggcgcatc gggcgctggg ccggatcggt ccaatagacc tcgggaacgg cgtcggcgag
      481 ggacgccgcg acgaccgcgg ggtcggcggc ggcatacggg tcgaacacct cgggcgcgac
      541 tgagcgaagg cgcgacaggt gtgagaggcg gggcgacacc ccgccattgt gtccgctccc
      601 ccagccgggt cgaggtattc cgccatcgtc gggtgatggt ggaatacctc gaccccgcgg
      661 ctggggggac ctacggagca gcctcgagtt gggcggcaag ggccaggagg cggccttcct
      721 gacccatccc ggcgacgagt tgggcgccga ccggccaccc ttcgcgggtg agtccggccg
      781 ggaccgaggc cgccgggtgc ccgctgacgt tgaagatggt ggtgaaaccc accagcggtg
      841 tcgagacgat ctgcgaccgc agcgttcctt ggccatccag ctggcccagc ggcggcgggg
      901 ggacgggcag ggtcggagtg agcaggacgt cccagtcggc aaacatcgtg tccagcgccg
      961 cggtcactcg ctcccccgcc ctgagagcgc gcgccacgac gcggtcggtg gcccagcccg
     1021 acaggcgcac ggtggctttg gtccgcggct caagcaattc cgggtgttca acctgacctg
     1081 cctccacccg catcccggca tagaactgcg ggaggaacgc ggtggtcggc gccggccatc
     1141 gcggcgagat gtgctggacg tcgtgcccga gcccggccaa ccggtcagcc agcgcggcca
     1201 cggccgacac gacctgaggg tccaccttcc cgccgggcag cacgggggtg gtggtccacc
     1261 cgacccgcaa ccgacctggc ggagtggctg cggcctgggc gaacggcata cccgggggtg
     1321 ccaggcgcca gcgatcggtc ggcgttgctc cggagatgac gtcgaggacg agcgcggagt
     1381 cggccgccgt gcgagtgagc gcgccgagac agaccaaggc gaaccaatgc tcggtcaacg
     1441 gcgcgacgct cacccggccg cggctgggct tgaggccgac cagcccgcaa caggaggcgg
     1501 ggatccggat cgagccacca ccgtccgcgc ccatggccac cggcaccatc cccgcggcga
     1561 ccgccgccgc tgagccaccc gacgacccgc ccggcgagcg gctgaggtcc cacgggttgt
     1621 gggtccgccc gtgggccagg ctctcggtgt gcgggaactg cccgaactcc ggcatcgccg
     1681 tcttgccgac gatgaccgct ccagcggcgc gcaaccggcg aatc
//
LOCUS       DAOMZY010000454         1624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_85353_length_1624_cov_5.181644, whole genome shotgun sequence.
ACCESSION   DAOMZY010000454 DAOMZY010000000
VERSION     DAOMZY010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85353_length_1624_cov_5.181644"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="PLK64_16840"
     CDS             complement(<1..733)
                     /locus_tag="PLK64_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HPV81462.1"
                     /translation="MAPQGFDDKVAFVWKVADKLRGTFKQHEYGSVMLPLLVLRRMDA
                     VLAPTKAAVLAKANGMSQIGPGQDALLKKAAGRRFYNISPLTFATLLHDDKHLADNLA
                     AYISCLSKEAAEVIDAYAFLGKIERMDRAGILYAVLADFADLDLRPASVSNEAMGYIF
                     EELLRKFSEMSNETAGEHYTPREVIRLMVNLLISGQTATDLTSNPRPVRTVYDPAAGT
                     GGMLMTAMAHLKELNPAAIVSVFGQE"
     gene            complement(858..1286)
                     /locus_tag="PLK64_16845"
     CDS             complement(858..1286)
                     /locus_tag="PLK64_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPV81463.1"
                     /translation="MEQRISLITLAVRDLAVSRRFYVDGLGWEAFLDVDDVLMLRVGE
                     HLLLSLWASESFTTEVGQSTLGTGIAPITLAHNVSSDAEVDAVLEELARAGGQIVVLA
                     TRREWGGYSGYAADPDGYRWEIANAGNGPLTALVVPSATS"
     gene            complement(1319..>1624)
                     /locus_tag="PLK64_16850"
     CDS             complement(1319..>1624)
                     /locus_tag="PLK64_16850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81464.1"
                     /translation="APTINDERCARVAGEAAVAALGLERVDAECAPIMPSEDFGVFAR
                     HVPACFSFIGNGTVEGEGGTPLHSRSYDANDDVLAAGVAFYGAVVRAELAPLVVGPE"
BASE COUNT          266 a          551 c          553 g          254 t
ORIGIN      
        1 gttcctggcc aaagacgctg acgatggcag ccgggttgag ctccttgagg tgggccatcg
       61 ccgtcatgag catgccgcca gtcccggccg ccgggtcata gacggtgcgc accggcctcg
      121 gattgctcgt cagatcggta gcggtctggc cggagatgag caggttgacc atgaggcgga
      181 tgacttcgcg tggggtgtag tgctcaccgg cggtctcgtt gctcatctcg gagaacttcc
      241 gcagcagctc ctcgaagatg tagcccatcg cctcgttgct cacgctggcc gggcgcaggt
      301 cgaggtccgc gaagtccgcg agcacggcat acaggatgcc cgcgcggtcc atccgctcga
      361 tcttgccgag gaacgcgtaa gcgtcgatga cctccgccgc ctccttggac agacaactga
      421 tgtatgccgc gaggttgtcg gcgaggtgct tgtcgtcgtg caggagggtc gcgaaggtga
      481 gcggcgagat gttgtagaac cggcggccgg cggccttctt gagcagtgcg tcctggccgg
      541 ggccgatctg ggacatgccg ttggccttgg cgaggaccgc agccttggtg ggtgcgagga
      601 ccgcgtccat gcggcgcagg acgaggaggg ggagcatcac cgagccgtac tcgtgctgct
      661 tgaaggtgcc gcggagcttg tcggcgacct tccagacgaa cgcgaccttg tcgtcgaagc
      721 cttgcggtgc catgccctgc ctctcctgcc agccaatgcc ccgcccaacc taccggcggt
      781 gcgaggggtc ggggagacgt ccggcaggtt ggcgacgcgc cgggagcgcc cagcggaagg
      841 ctgtcagacg tcgcggctca gctagtggcg ctggggacaa cgagggcggt gagggggccg
      901 ttgccggcgt tggcgatctc ccagcgatag ccgtccgggt cggccgcgta gccggagtag
      961 ccaccccact cgcggcgcgt ggccagcacc acgatctggc caccagcccg cgccaactcc
     1021 tccagaaccg catcgacctc ggcatcgctg gagacgttgt gtgccagcgt gatcggagcg
     1081 atgccggtgc caagagtgct ctgcccgacc tcagtggtga aggactcgct tgcccagaga
     1141 ctcaggagca gatgctcgcc cactcgcagc atgaggacgt cgtcgacatc gaggaatgcc
     1201 tcccacccga ggccgtcgac atagaagcga cgcgagacgg cgaggtcccg gacggcgagc
     1261 gtgatgaggc tgatccgttg ctccatgcct cgatcctgcc cggcccaccc ccagaggact
     1321 actccggccc aaccaccaac ggcgccaact cggcccgcac aacagcgcca tagaacgcca
     1381 cccccgccgc cagcacgtca tcgttggcgt cgtacgaccg gctgtgcagc ggcgtcccgc
     1441 cctcgccctc gaccgtcccg ttgccgatga acgagaagca cgccgggacg tgccgcgcaa
     1501 agaccccgaa gtcctcgctc ggcatgatcg gcgcgcactc ggcatcgacg cgttcaaggc
     1561 cgagcgccgc cacggccgcc tcaccggcca cccgcgcgca ccgctcgtcg ttgatcgtcg
     1621 gcgc
//
LOCUS       DAOMZY010000455         1541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Dermatophilaceae bacterium isolate AMR_MDS_3987
            NODE_93133_length_1541_cov_6.580754, whole genome shotgun sequence.
ACCESSION   DAOMZY010000455 DAOMZY010000000
VERSION     DAOMZY010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865958
            Sequence Read Archive: SRR5007313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dermatophilaceae bacterium (wastewater metagenome)
  ORGANISM  Dermatophilaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:53:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,372
            CDSs (total)                      :: 3,334
            Genes (coding)                    :: 3,317
            CDSs (with protein)               :: 3,317
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Dermatophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93133_length_1541_cov_6.580754"
                     /isolate="AMR_MDS_3987"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2728912"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-29"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /gene="rpsB"
                     /locus_tag="PLK64_16855"
     CDS             complement(<1..773)
                     /gene="rpsB"
                     /locus_tag="PLK64_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPV81465.1"
                     /translation="MAVVTMRQLLESGVHFGHQTRRWNPKMKRFIMTERNGIYIIDLQ
                     QSLTHINDAYEFVRETVAHGGSILFVGTKKQAQEPVAEQAARVGMPYVNHRWLGGMLT
                     NFTTISKRLQRLKELEEIDFDDVAGSGHTKKELLILKREKDKLEKTLGGIRTMARVPS
                     AVWIVDTKKEHLAVDEARKLGLPVIAILDTNCDPDEVDYKIPGNDDAIRSVALLTRVI
                     ADAVADGLVARSSVRTGESAEGVAADEPLAEWERELYAEA"
     gene            1244..1435
                     /locus_tag="PLK64_16860"
     CDS             1244..1435
                     /locus_tag="PLK64_16860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPV81466.1"
                     /translation="MSALSRNHLLALAAIGLALFVVAIGFAAAKAEVSPTVLVVSALV
                     AAAAGGAVIAAILRGPGRS"
BASE COUNT          206 a          507 c          532 g          296 t
ORIGIN      
        1 gcctcggcgt agagctcgcg ctcccactcg gccagcggct cgtcagcggc gacaccctcg
       61 gcggactcgc cggtgcggac gctcgagcgc gccacgagac cgtcggcgac ggcgtcggcg
      121 atgacccggg tcagcagggc caccgagcgg atggcgtcgt cgttgcccgg gatcttgtag
      181 tcgacctcgt cggggtcaca gttggtgtcg aggatcgcga tgaccggcag cccgagcttg
      241 cgagcctcgt cgacagccag gtgctccttc ttcgtgtcga cgatccacac ggccgaaggg
      301 acccgagcca tcgtgcggat gccgccgagg gtcttctcga gcttgtcctt ctcgcgcttg
      361 aggatgagca gttccttctt cgtgtggccc gaaccggcca cgtcgtcgaa gtcgatctcc
      421 tcaagctcct tgaggcgctg cagccgcttg gagatggtcg tgaagttggt gagcatgccg
      481 ccgagccagc ggtggttgac gtagggcatc ccgacccgcg cggcctgctc ggccacgggc
      541 tcctgggcct gcttcttcgt gccgacgaac aggatcgacc cgccgtgggc aaccgtctcg
      601 cggacgaact cgtaggcgtc gttgatgtgg gtcagcgact gctgcaggtc gatgatgtag
      661 atgccgttgc gctcggtcat gatgaagcgc ttcatcttgg ggttccaccg gcgggtctgg
      721 tgcccgaagt ggacgccgct ctcgaggagc tggcgcatgg taacgacggc catgccgtgt
      781 tctcctatgt gtcgttgtca gttacgtcgg taggccgcgg ggtccgcgcg cgaggcgcgg
      841 gtgcggcata cctgcctggc gtcctgactc accccgccgg tatgccgtgt ggccgaccgg
      901 actgccgtgg tgcgccttcg gtggtcatgg gaccgccgac gagggacgcg cgaaatggtg
      961 ctgcgatggt gccgatggcc cggaggccat cggccagtct acgtcctggg ccgggtccgc
     1021 cggtaatcgc cgtgccacgc cccgtgtatc gaagctgcgc ggctcagctg tcgaacccga
     1081 cccgcgccgg tcgtggcttg gcgcgccgta caccgggtga cacactcgtc gtagggcatc
     1141 aacctgccat ggcaccaagg tgtcagccgt tccccctttg tcaatctccg acccgcccat
     1201 agcgtccttg tgggtagcgg cgcttcgggc ccgaggagga gttgtgtctg cgctttccag
     1261 gaaccacctg ctcgcattgg cggccatcgg attggccctg ttcgtggtgg cgatcgggtt
     1321 cgccgcagcc aaggcggagg tttcgccaac cgtcctcgtg gtctccgccc tcgtcgccgc
     1381 tgctgccggc ggggcagtga tcgcggccat cctgaggggc ccaggccgta gctgagccct
     1441 cctccccgac caacggcctg aagcgagttg ctggctgcgt caattcctga ggagccaggc
     1501 cgacgccggt cgctccgtgt tgttgtcgag ccacctctag g
//
